LOCUS       DASZZC010000001         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1092319,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000001 DASZZC010000000
VERSION     DASZZC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092319"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VHE37_00005"
     CDS             complement(<1..591)
                     /locus_tag="VHE37_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HVT33933.1"
                     /translation="MSVFSHPEFDAHELVAFHHDRASGLKAIIAVHNTALGRGLGGCR
                     MWPYRNDGEALNDVLRLSRGMTYKAALAGLPQGGGKSVIIGDPRQDKNEKLIRAMGRF
                     VDSVGGKYVVAEDSGTSVADIRLMAQETRHVGGLADDKSVAAGRTGDPSPATAYGVFV
                     GIKASVKAQYGRDDLKGLRVAIQGCGNVGYRLAQHLH"
     gene            complement(790..1572)
                     /locus_tag="VHE37_00010"
     CDS             complement(790..1572)
                     /locus_tag="VHE37_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HVT33934.1"
                     /translation="MTYCVAIKVNDGLVFAADTRTSAGVDDVRTYNKLHTFELGDRIF
                     VLLSAGNLATTQAVLAHARREIDDPNAPVSLRTVRHMYDAAEYLGQLSVRAQRSATQE
                     GQIGGVNVETTLILGGQIQNEDPAIYLIYPLGNCIAASPETPYLQIGESKYGKPILDR
                     IIRPHITLEDSARCALVSLDSTMKSNISVGPPLDVVIVPRNAISVARRMRLDLDTPYY
                     ASLKRAWGEQLERVFRELPRFEWEAQVQQQQLPLQSQAQGPY"
BASE COUNT          254 a          555 c          552 g          325 t
ORIGIN      
        1 gtgcaggtgc tgcgccaggc ggtagccgac gttgccgcag ccctggatgg ccacgcgcag
       61 acccttgagg tcatcgcgcc cgtactgcgc cttcaccgag gccttgatgc cgacgaacac
      121 gccgtacgcg gtggccggtg acggatcgcc ggtgcgcccc gccgccacgc tcttatcgtc
      181 cgccaggccg ccgacatgcc gcgtttcctg cgccatcagc cggatatcgg ccacgctggt
      241 gccggaatct tccgccacca cgtacttgcc gccgacgctg tcgacgaagc ggcccatggc
      301 gcggatcagt ttttcgttct tgtcctggcg cggatcgccg atgatcacgc tcttgccgcc
      361 gccctgcggc agcccggcca gcgcggcctt gtaggtcatg ccgcgcgaca ggcgcagcac
      421 gtcgttcagc gcctcgccgt cgttgcggta cggccacatg cggcagccgc ccagcccgcg
      481 gcccagcgcg gtgttgtgca cggcgatgat ggctttcagg cccgacgcgc ggtcgtggtg
      541 gaaggcgacc agctcgtgcg cgtcgaattc ggggtgcgag aaaacggaca tggtttttgt
      601 gctcctgcgt gtgcgcattt taatccgcgc gtcctgcgcg gcaggcggga gcggaaaaaa
      661 tggccaaccg caccgcggtt ccgcctgtta ttaattattt tattatttta cttacatata
      721 atattttata acaccaccag ccggggtgcg gtggatgctt ttttcgcccc gccgcgggcg
      781 ccggcccgct cagtacggtc cctgcgcctg cgattgcagc ggcagttgct gctgctgcac
      841 ctgcgcttcc cactcgaagc gcggcagctc gcggaacacg cgctccagct gctcgcccca
      901 ggcccttttc agcgacgcgt agtacggcgt gtccaggtcc agccgcatgc gccgcgccac
      961 cgagatggca ttgcgcggca cgatcaccac gtccagcggc gggccgacgg agatgttcga
     1021 cttcatggtc gagtccagcg acaccagcgc gcagcgcgcg gaatcttcca gcgtgatgtg
     1081 cgggcggatg atgcggtcga gtatcggctt gccgtacttg ctctcgccga tctgcaggta
     1141 gggcgtctcc ggcgacgcgg caatgcagtt gcccagcgga tagatgagat agatggccgg
     1201 gtcttcgttc tggatctgcc cgcccaggat cagcgtggtc tcgacgttga cgccgccgat
     1261 ctgcccttcc tgcgtggccg agcgctgcgc gcgcaccgac agctggccga ggtattccgc
     1321 cgcgtcgtac atgtggcgca cggtgcgcag gctgaccggc gcattggggt cgtcgatctc
     1381 gcgccgcgca tgtgccagca cggcctgggt ggtggcgaga ttgcccgccg acagcagcac
     1441 gaaaatgcgg tcgcccagct cgaaggtgtg cagcttgttg taagtgcgca cgtcatcgac
     1501 accggcgctg gtgcgcgtgt cggcggcaaa cacgaggccg tcattgacct tgatggcgac
     1561 gcaatacgtc atcgaatcgt tctggggtaa tggtctgccg attttacgga ttcaaccgcc
     1621 gccggtgttg cgccgctgca cggctcaacg ccgccgcagg tcgagggtcc atgggaattc
     1681 cgcgtt
//
LOCUS       DASZZC010000002         4772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1220817,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000002 DASZZC010000000
VERSION     DASZZC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220817"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..254
                     /locus_tag="VHE37_00015"
     CDS             78..254
                     /locus_tag="VHE37_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33935.1"
                     /translation="MKDKKAKPAKKHTAMKLPQTAQASRNPMANAGSQSWQHGGGKPH
                     KSPEHLMGKARKVH"
     gene            complement(349..1590)
                     /locus_tag="VHE37_00020"
     CDS             complement(349..1590)
                     /locus_tag="VHE37_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HVT33936.1"
                     /translation="MMSWSEIGHDLSQHWYLYASMPLVAGTIGYVTKLVAIRMMFEPI
                     EFTGCRPLLGWQGIVPRNAERMAGIACDTMTSQLIKPADIFGRLDPLRVAQELEQPML
                     AAVEEITREVAAEYQPGLWEAMPEMLRRRLVQRIQDDAPNLAAQIMLDIKINLDQVFD
                     LRDMVVTNLTRDKKLLNRIFQEAGDKEFTFIARSGIWFGGIIGCVQAVVWALTHSALV
                     MPVFGLFTGWFTDWLALRMVFRPQEPTRYVFGLVEWQGLFLKRRKEVAAAYGALIASE
                     ILTPRNIINAVLSGPLSDRLVSLVSKHVQNAVDQQAGLARPLVVLAVGSKRYQEMKKV
                     IAAKLVTRMPEALQKLENYAEAAMDVRNTLITKMQQLDAAQFERLLRPAFEQDEWILI
                     AVGAALGFLVGELQVFLMLHL"
     gene            complement(1600..3720)
                     /locus_tag="VHE37_00025"
     CDS             complement(1600..3720)
                     /locus_tag="VHE37_00025"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT33937.1"
                     /translation="MRVNQGWLLGGLLLTLPAYALDFQYGDDLSFTLNNHLSAGVQLR
                     TQDRDYALIGKENVPGQEHLCDADNCISLNRDPAPNQRLVASQGAYSGVNGDNGDLNY
                     DKYDITAATFKLDSDLTAKWGEHWLARFRAIGYYDPVNANFNEHHFDTRFQPEYTKRP
                     DDVARAFAKGVKLYDAYGQYSFDWNGNSAAVSVGNQLVRWGESTLVALNSLSEINPPN
                     SAIFHMPGYEINEVFQPVPVALFSTDVVKNVSAEVLYQFGWKPVIADPAGSFMADEDV
                     LGHSHAPLYITLGQFGEDPNRQLHFAPTLGYVAGGTVTSYLGDPREPQNGGQYGLRLN
                     YFADWLNGGTETSFYYLNYHSRLPYLSAIATDDSCARDASNTAVALVDCRGFNGSINP
                     TGLGLDPLPVQSLKVFLDYPENIHMFGFSFNTNIGEYSLAGEFAYRPNVPLQVQITDV
                     VFAGLQPAFPANELTAPVIGSPITSLPGLPLNAQDIALLLESDFPSVQQASPSFLADY
                     RHLGRIHAHQLIEGWQRMHVGQFDFTAIKATSTNPLGADQIIFIGEVGGTYIFNMPSL
                     SQLQFEGGGPYRTSYSPGADGTGTPDGQPDARHLNPHQQTHGFADAFSWGIRSITRFE
                     YNDVVFGWSFKPQIVFAWDISGIAPLPIQNFIEGRKVIEAGTDINITQALTARAVYEW
                     YTGGGEDNTRKDRDNLALSLSYSF"
     gene            3906..>4772
                     /locus_tag="VHE37_00030"
     CDS             3906..>4772
                     /locus_tag="VHE37_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HVT33938.1"
                     /translation="MRDSGQLLAVVHAGMVKAGLDVDAIYTRLGYDAERLDLRALRTR
                     HQAQVLFWHTVETVTGDADIGLHLCPHLPVFRGDVLEYLVFSSPTFGEGCRRALKYLR
                     LVSDAIAMRLVQDAQGSRLAVVGSKLDAPQLRHTEICVVYELIRFAQSVTEGEFRAHK
                     VKLRFEPRSLAAEYKKVFGCPVEFGGSESEIWFDPKVLAYRSPHWDPDLLQLHEELAE
                     KRLSTLRRADLIDQIRRIFSQRLELETLELEDVARELGMPPRRLRFELTRAGTSFSDL
                     LAGFRYALARRLL"
BASE COUNT          831 a         1536 c         1595 g          810 t
ORIGIN      
        1 cgcgtgtacg gcgtcgaagt ggatatcatt gaatgcggcg ccggccggcg cggatggatc
       61 gcagtgacag gacgaacatg aaagacaaga aggccaagcc cgccaaaaaa cacacggcga
      121 tgaaactgcc gcagaccgcg caggcttcgc gcaacccgat ggcaaatgcc ggtagccaat
      181 cctggcagca tggcggcggc aagccgcaca agtcgcccga gcacctgatg ggcaaggcgc
      241 gcaaggttca ttgagccgtc cctggaccag gaacgcgcca tccctggcgc gcaccgtcaa
      301 agcacgtgaa agcaggagac tgctcgcgtg catcgcatgc aggggctcct acagatgcag
      361 catcaagaac acctgtagct cgccgaccag gaaacccagc gccgcgccga ccgcgatgag
      421 aatccactcg tcctgttcga atgccggccg cagcaggcgc tcgaactgcg ccgcgtcgag
      481 ctgctgcatc ttggtgatga gcgtgttgcg cacgtccatc gccgcctcgg cgtagttctc
      541 cagcttctgc agcgcctcag gcatgcgcgt gaccagcttg gccgcgatca ccttcttcat
      601 ttcctggtag cgcttgctgc ccaccgccag caccaccagc gggcgcgcca gtccggcctg
      661 ctggtccacc gcgttctgca cgtgcttgct gaccagcgac accagccgat ccgacaaggg
      721 cccgctgagc acggcgttga tgatgttgcg cggcgtgagg atttccgagg cgatcagcgc
      781 gccgtaggcc gcggccactt ccttgcgccg cttgagaaac agcccttgcc attccaccag
      841 gccaaacaca tagcgcgtcg gctcctgggg ccggaacacc atgcgcagcg ccagccagtc
      901 ggtgaaccag ccggtgaaca ggccgaacac cggcatgacc agcgccgagt gcgtcagcgc
      961 ccacaccacc gcctgcacgc agccgatgat gccgccgaac cagatgcccg agcgcgcgat
     1021 gaaggtgaat tccttgtcgc cagcctcctg gaagatgcgg ttgagcagct tcttgtcacg
     1081 cgtgaggttg gtcaccacca tgtcgcgcag atcgaacacc tggtccaggt tgatcttgat
     1141 gtcgagcatg atctgcgccg ccaggtttgg cgcgtcgtcc tggatgcgct gcaccaggcg
     1201 cctgcgcagc atttccggca tggcctccca cagcccgggc tggtactcgg cggcgacctc
     1261 gcgcgtgatt tcctccaccg ccgcgagcat cggctgctcc agctcctgcg ccacgcgcag
     1321 cggatcgagc cggccgaaga tgtccgccgg cttgatcagc tgcgaggtca tggtgtcgca
     1381 ggcgatgccg gccatgcgct cggcattgcg cggcacgatg ccctgccagc ccagcaacgg
     1441 ccggcagccg gtgaactcga tcggctcgaa catcatgcgg atcgccacca gcttggtcac
     1501 gtagccgatg gtgccggcca ccagcggcat cgatgcgtag agataccagt gctgcgacag
     1561 atcgtggccg atctcgctcc agctcatcat gggcgcggac tagaacgagt acgacagcga
     1621 cagcgccagg ttgtcgcggt ccttgcgcgt gttgtcctcg ccgccgccgg tgtaccactc
     1681 gtaaaccgcg cgcgcggtga gcgcctgggt gatgttgatg tcggtgccgg cctcgatcac
     1741 cttgcgcccc tcgatgaagt tctggatcgg cagcggcgcg atgccgctga tgtcccaggc
     1801 gaacacgatc tgcggcttga agctccagcc gaacaccacg tcgttgtatt cgaagcgcgt
     1861 gatgctgcgg atgccccagg aaaacgcatc ggcgaagccg tgcgtctgct ggtgcggatt
     1921 gagatggcgc gcatcgggct ggccatcggg cgtaccggtg ccgtcggcgc ccggcgaata
     1981 cgaggtgcgg tacggaccgc cgccctcgaa ctgcagctgg ctgagcgacg gcatgttgaa
     2041 gatgtaggtg ccgccgactt caccgatgaa gatgatctgg tcggcgccga gcggattggt
     2101 cgaggtggcc ttgatcgccg tgaagtcgaa ctgccccacg tgcatgcgct gccagccttc
     2161 gatcagctgg tgcgcgtgga tgcgccccag gtggcggtag tcggccagga acgacggcga
     2221 cgcctgctgc accgagggga aatcggattc cagcagcagc gcgatgtcct gcgcattcag
     2281 cggcagcccc ggcagcgagg tgatgggcga gccgatcacg ggcgcggtca gctcattcgc
     2341 ggggaacgcc ggctgcaggc cggcaaacac cacgtcggtg atctgcacct gcagcggcac
     2401 gttgggccgg taggcgaact caccggccag cgagtactcg ccgatgttgg tgttgaacga
     2461 gaagccgaac atgtggatgt tctcgggata gtcgaggaac accttgagcg actgcaccgg
     2521 cagcggatcg aggcccaggc cggtgggatt gatcgagcca ttgaagccgc ggcaatcgac
     2581 cagcgcgacc gccgtattgc tggcatcgcg cgcgcaggaa tcgtcggtgg cgatggccga
     2641 aagatacggc aggcggctgt gatagttcag gtaatagaaa ctggtctcgg tgccgccgtt
     2701 gagccagtcg gcgaagtagt tgaggcgaag gccgtactgg ccaccgttct gcggctcgcg
     2761 cggatcgccg aggtatgagg tgacggtgcc gccggcgacg tagcccagcg taggcgcgaa
     2821 gtgcagctgg cggttcgggt cttcgccgaa ctggccaagc gtaatgtaca gcggcgcatg
     2881 cgaatggccg agcacgtcct cgtcggccat gaagctgccg gccgggtcgg cgatcaccgg
     2941 cttccagccg aactggtaca gcacctcggc cgacacgttc ttgaccacgt cggtggagaa
     3001 caacgccacc ggcaccggct ggaacacctc gttgatctcg tagccgggca tgtggaagat
     3061 cgccgagttc ggcgggttga tctccgacag gctgttgagc gccaccagcg tgctctcgcc
     3121 ccagcgcacc agctggttgc ccaccgagac cgcggcgctg ttgccgttcc agtcgaacga
     3181 atactggccg taagcgtcgt acagcttcac gcccttggcg aaggcgcgcg ccacgtcgtc
     3241 gggccgcttg gtgtactcgg gctggaagcg cgtgtcgaaa tggtgctcgt tgaaattggc
     3301 gttgaccgga tcgtagtagc cgatcgcgcg gaagcgcgcc agccagtgct cgccccactt
     3361 ggcggtcagg tcggaatcga gcttgaaggt ggcggcggtg atgtcgtact tgtcgtagtt
     3421 gagatcgccg ttgtcgccgt tgacgccgga ataggcgccc tgcgaggcca ccaggcgctg
     3481 gttcggcgcc gggtcgcggt tgagcgagat gcagttgtcg gcatcgcaca ggtgttcctg
     3541 gcccggcacg ttctccttgc cgatcagcgc gtagtcgcgg tcctgcgtgc gcagctgcac
     3601 gccggcggac aggtggttgt tgagggtgaa gctcaggtca tcgccatact ggaaatccag
     3661 tgcgtaggcc ggcagcgtga gcagcaggcc gccgagcagc catccttggt ttacgcgcat
     3721 tcgggttctc ctcccagttg cacggttctg tttgccgcaa gtctggcaaa gtatcgggcg
     3781 cggcgcatct gtatgcgcgt acgccgcagg aaaaataagc catccggccg tccggcgcga
     3841 tggcccacgg tgccggaagc accgtcgttt cgggccagcg ccgccgcggt caacttgggg
     3901 aggacatgag ggactccggt cagctgctcg cagtggtcca tgccggcatg gtcaaggccg
     3961 ggctcgacgt ggacgccatc tacacgcgcc tgggctacga cgccgagcgc ctggacctgc
     4021 gcgcgctgcg cacgcgccac caggcacagg tgctgttctg gcacacggtc gaaaccgtca
     4081 ccggcgacgc ggatattggt ctgcatctct gccctcacct gccggtgttc cgcggcgacg
     4141 tgctcgagta cctggtgttc tccagtccca ccttcggcga aggctgccgg cgcgcgctga
     4201 agtacctgcg cctggtgtcc gacgccatcg ccatgcggct ggtgcaggat gcgcagggca
     4261 gccgcctggc ggtggtcggc agcaagctcg acgcgccgca gctgcgccac accgagatct
     4321 gcgtggtgta cgaactgatc cgcttcgcgc aatcggtgac cgaaggcgaa ttccgcgcgc
     4381 acaaggtcaa gctgcgcttc gagccgcgca gcctggcagc ggaatacaag aaggtgttcg
     4441 gctgcccggt cgagttcggc ggcagcgaaa gcgaaatctg gttcgacccc aaagtgctgg
     4501 cctaccgctc gccgcactgg gacccggatc tgctgcagct gcacgaggaa ctggcggaga
     4561 agcgcctgtc cacgctgcgc cgcgccgacc tgatcgacca gatccgccgc atcttctcgc
     4621 agcgcctcga gctggaaacg ttggaactgg aagacgtggc gcgcgagctc ggcatgccgc
     4681 cgcggcgcct gcgtttcgaa ctcacgcgcg ccggcaccag cttctccgac ctgctcgccg
     4741 gctttcgcta cgcgctggcg cggcggctgc tg
//
LOCUS       DASZZC010000003         8748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_707044,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000003 DASZZC010000000
VERSION     DASZZC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707044"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VHE37_00035"
     CDS             complement(<1..489)
                     /locus_tag="VHE37_00035"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVT33939.1"
                     /translation="MKKLLALTLCWSALICGLVFVSASAEAGDMPPGYAVASAHPLAT
                     QAGLDILAAGGNAFDAAVAVSAALGVVEPTGSGLGGGGFFLLHRASDGLDTFVDGRET
                     APRKATANMYLDASGKADAQRSLVGMLASAIPAQPALLDYVARKYGSKPLADDLAPAI
                     RYA"
     gene            complement(510..776)
                     /locus_tag="VHE37_00040"
     CDS             complement(510..776)
                     /locus_tag="VHE37_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HVT33940.1"
                     /translation="MALKITSACINCDVCEPVCPNHAIYQGVDIYEINPDLCTECVGH
                     FDAPECQKICPVACIPLDPDHAETREQLEAKYMRLIGAAKASLG"
     gene            complement(872..1831)
                     /locus_tag="VHE37_00045"
     CDS             complement(872..1831)
                     /locus_tag="VHE37_00045"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HVT33941.1"
                     /translation="MAFSRIVGTGSYLPAQVLSNRDLELRSDTTDEWIFSRTGIRSRH
                     VAAPEEKTSDLALAAARKAMEAAQVSADDIDLIVVATSTPDLVFPSTACLLQQKLGVT
                     RGAAFDVQAVCSGFLYALTVADKFVAGGQFRCALVIGAEVFSRLLDWSDRRTSVLFGD
                     GAGAVVLKPHAEHGILSTHLHSDGNYSHILRASGFVQDGQLHDHAFVTMDGQAVFRFA
                     VRVLEEVCKEALAATGYKGDELDWLVPHQANIRIIQATAQKLGMRPERIVTTLETQGN
                     TSAASVPLALDAAVRDGRIQPGHKVMLEAVGAGMTWGAAMVRW"
     gene            complement(1903..2436)
                     /gene="coaD"
                     /locus_tag="VHE37_00050"
     CDS             complement(1903..2436)
                     /gene="coaD"
                     /locus_tag="VHE37_00050"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HVT33942.1"
                     /translation="MSVVAAYSGTFDPITLGHYDIIQRAAAMFPRLIVAVGANIAKNP
                     RFNMDERVALIREVVKELRNVEVKGFSGLVVDFARDNGVTVLVRGVRTVGDVDYEKQM
                     AVMNRDLYPKLDTVMLAPSPEYAHLSSSLVRELAVLGAPIEKLVPAPVVPVLKVRATA
                     GVTAPANGTRRRRGRAR"
     gene            complement(2433..2996)
                     /gene="rsmD"
                     /locus_tag="VHE37_00055"
     CDS             complement(2433..2996)
                     /gene="rsmD"
                     /locus_tag="VHE37_00055"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HVT33943.1"
                     /translation="MKRAPGKLRVIGGEWRSRLVEFDGDSGVRPTPDRVRQTVFDWLA
                     PLIEGTRCLDLFAGSGALGIEALSRGARKVTFVENGRTQISLIHAALLQFKADHRFDM
                     RPMDALSYLEITATRYNVVFLDPPYESDLLGRALERLPSRLLPDNRVYLEWRGGAPPL
                     PPGYELLREKQAGQVSYGLASYRMEKT"
     gene            complement(3030..3494)
                     /locus_tag="VHE37_00060"
     CDS             complement(3030..3494)
                     /locus_tag="VHE37_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33944.1"
                     /translation="MIQLRFSSDLDAAPADVWRHASSMAGVNHELLPLMRMTHPRDAT
                     DLSSQLLKLGQTAFVSTLLLFGLLPADRHHLMLERLYPGVGFDERSWSWLQRVWIHRR
                     RIEQHGEVTRITDELEFEPRLFFMAPLLRPVIRALFAHRHRRLRKKFLRRAA"
     gene            3550..4386
                     /locus_tag="VHE37_00065"
     CDS             3550..4386
                     /locus_tag="VHE37_00065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT33945.1"
                     /translation="MAAKSENVSPTAYATGYLWYRNGMSHEALATEQGRKVDRWFHGF
                     IAATRALTGVQLDTMLIARHKGIDGELARAIEAGRVGQVVEIAAGLSPRGTTFCQRYP
                     KLTYIETDLPKMAALKRSLLDKGGLLSARHRVQVVDALAQRGANSLAALAKTLDAQQG
                     VAVITEGLMSYLDPVAAHHVWRNTAHFCKRFPHGLYLSDAYLAGDRSGAAFAAFGLMI
                     SAFVRGRLHRQFRSAEHARERLARAGFTDVRMLQTSEIPETRELSKLRGPDRVRVLEA
                     ST"
     gene            4473..7448
                     /locus_tag="VHE37_00070"
     CDS             4473..7448
                     /locus_tag="VHE37_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT33946.1"
                     /translation="MSIRLGCSALVMFFAPAIYAQDATTPTAPPPQSAESPTVLDTVQ
                     VTGTRLARPGYSTPTPTTSVTARDIRDSGTTNLGDYLDNLPQLSSSANMANSTQFIGT
                     AGLNELDLRDLGTSRTLVLVNGHRHVGALGGDTAVDINTIPQDLVERVDFITGGASAI
                     YGADAVTGVVNFVLTQNFEGLSVRAQTGTNWDGGYDKNLVAITAGTNFAGDRGNFVLA
                     GEFAQQSRLTAKERPSMALQRSYLQNPLDVDGNGNPIEKPNRRPNMILVPGAGTNETS
                     EGGTVLTAGYINDGNIRDAYLFDPGGNFRRVRIDGIVDAGNELCSDCDYLDENAYQDL
                     QPAFDRYNLTAMSHFQISDWARLDVEGNFAHTRALTYFFPSFDDVFQGNAIAIQPDNA
                     YMPAGLQAFFTSHNKREVMVNRIDADAGLRAEKDERDMTQVTLALTGNLPFSPTWNYN
                     LSAVYGHTRIDTTFINNRINDRFSEATDAVVDPMTHNIVCRATIDSKAKLPGTNTPVP
                     ADARVGCIPTNVFGAGAINQASANWFNATGLETDGVSLAVYTAYLVNDALIDLPAGPL
                     AAALGTEYRLEQSNTLPDALSATGATFNNILLPLSGGFHAWDGFAETSVPLIGGIPLV
                     KLLELELAARYSSYNTIGSTLSSKVGLNWTVAPDLRIRSTFARAVRAPNIGELYSPAS
                     QDFFFVNDPCSQKNVKNAPDPAIRSNNCAALNIPADFHSHADADTIPGVSGGNPDLTE
                     ERATTWTAGAVLTPRWITGWSVSVDFWDISINNAIDLLDAQDILNKCVDDAGGPNTQF
                     CALVTRDPTSHEITNIVDIEENVQRLTARGVDFESSYKFPIAGGSTEFHLVGTYLNQL
                     RTYPFQSEPKRSFEAAGVLGDPRWQINLDTRYDHGPLSFTWSIRWIDKQYLVDRDTLQ
                     AHPDAQEPIRAGTRFYHDVQARYHLPGSMKAVEVYAGVDNLLNADIPTAGSLLGVGTG
                     SAIYDNVGRFLYAGVEYKI"
     gene            7489..7998
                     /locus_tag="VHE37_00075"
     CDS             7489..7998
                     /locus_tag="VHE37_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HVT33947.1"
                     /translation="MNHAIWSVDWHALFVPTGSLLEIFIRGSVIYLLLFFLMRFLRRE
                     AGQIGIADVLVIVVIADAAQNGMAGTYTSITEGVVLIATIMGWDYLLDWLDFHWPAFA
                     RLVHPAPVPLVVDGRVLWKNLRRQLITESELRSRMREEGIEHLESVKRCYMEGDGRIS
                     VIGHGGGAR"
     gene            complement(8120..>8748)
                     /gene="gpmA"
                     /locus_tag="VHE37_00080"
     CDS             complement(8120..>8748)
                     /gene="gpmA"
                     /locus_tag="VHE37_00080"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HVT33948.1"
                     /translation="KLMREEGLMFDVAHTSVLKRAINTLMVALTEMDQLWLPVQKSWR
                     LNERHYGALQGLDKAETTAKYGEAQVKIWRRSYDIPPPPMAEDNPQHPRFDRRYGKLD
                     QSALPATESLKTTLERVLPYWHDAIAPQLKSGQTVLVTAHGNSLRALYKYLNNISDEA
                     IVELNIPTGIPLLFELYDNLKVKSYRYLGDPEAAKKAAEAVANQAKAK"
BASE COUNT         1565 a         2867 c         2766 g         1550 t
ORIGIN      
        1 cgcgtagcgg atggccggcg cgagatcatc ggccagcggc ttgctgccgt acttgcgcgc
       61 cacgtagtcg agcagcgccg gctgcgccgg gatggcgctg gccagcatgc cgaccagcga
      121 gcgctgcgca tcggccttgc cgctggcatc gagatacatg ttggcggtgg ccttgcgcgg
      181 cgccgtctcg cggccgtcga caaacgtgtc gagcccgtcg ctcgcgcggt gcagcaggaa
      241 gaagccgccg ccgcccaggc ccgagccggt gggctccacc acgccgagcg cggcggacac
      301 ggcgaccgcg gcgtcgaagg cgttgccgcc ggcggcgagg atgtcgagac cggcctgcgt
      361 cgccagcgga tgcgcgctgg ccaccgcgta gccgggcggc atgtcgccgg cttcggcgct
      421 cgcggagacg aacaccagcc cgcagatgag tgcggaccag cagagagtca gcgcgaggag
      481 tttcttcatc tgcgtcactg gtccgggcgt cagccgagcg acgccttcgc cgcgccgatc
      541 agccgcatgt acttggcttc gagctgctcg cgcgtttcgg cgtggtcggg gtcgagcggg
      601 atgcacgcca ccgggcagat cttctggcat tccggcgcgt caaaatggcc gacgcactcg
      661 gtgcacaggt cgggattgat ctcgtaaatg tccacgccct ggtagatggc gtggttgggg
      721 cacaccggct cgcacacgtc gcagttgatg caggcgctgg tgatcttgag ggccatgcgc
      781 gcattttact caccccaaag ccgtctcagt gagcgtcgcg agcgacagca gattgcacga
      841 ggacggagcc cagcagttca acgaggcggc ttcaccagcg caccatcgcc gcaccccagg
      901 tcatgccggc accgaccgct tccagcatca ccttgtgccc gggctggatg cggccgtcgc
      961 gcaccgcggc gtcgagcgcc agcggcaccg acgcggccga ggtgttgccc tgcgtttcca
     1021 gcgtggtgac gatgcgctcg ggccgcatgc cgagtttctg cgcggtggcc tggatgatgc
     1081 ggatgttggc ctggtgcggg accagccagt cgagctcgtc gcccttgtag ccggtggcgg
     1141 cgagcgcctc cttgcacact tcttccagca cgcgcacggc gaagcggaac acggcctggc
     1201 catccatggt gacgaaggcg tggtcgtgca gctggccgtc ctgcacgaaa cccgaggcgc
     1261 gcaggatgtg gctgtagttg ccgtcggaat gcaggtgcgt cgacaggatt ccgtgctcgg
     1321 cgtggggctt gagcaccacg gcgccggcac cgtcgccgaa cagcacgctg gtgcggcggt
     1381 cgctccagtc gagcaggcgc gagaacactt ccgcgccgat gaccagcgcg cacctgaact
     1441 gcccgccggc gacgaacttg tccgccaccg tcagcgcgta gagaaagccc gagcacaccg
     1501 cctgcacgtc gaacgccgcg ccgcgcgtga cacccagctt ctgctgcagc aggcaggcgg
     1561 tcgaggggaa caccaggtcg ggcgtcgagg tggcgaccac gatcaggtcg atatcgtcgg
     1621 cgctgacctg cgcagcctcc atcgccttgc gcgccgcggc cagcgcaaga tcggaggtct
     1681 tttcttcggg cgcggccacg tggcggctgc ggatgccggt gcgcgagaag atccactcgt
     1741 cggtggtgtc gctgcgcagt tcgagatcgc ggttgctcag gacctgggct ggcaggtaac
     1801 tgccggtgcc gacgatgcgc gaaaaggcca tgagtgccgc tctctgctgg aagccccgcg
     1861 tgctgggggc gccatttcag caaatttccg gcaagtgtgc gttcagcgcg cgcggccgcg
     1921 gcgccgtctg gttccgttgg ccggcgcggt cacgccggcg gtggcacgca ctttgagcac
     1981 cggcaccacc ggcgcgggca ccagcttttc gatcggcgcg ccgagcaccg ccagttcgcg
     2041 caccagcgac gacgacaggt gcgcgtactc aggcgatggc gccagcatca cggtgtccag
     2101 tttcggatac aggtcgcggt tcatcaccgc catctgcttt tcgtaatcga cgtcgcccac
     2161 cgtgcgcacg ccgcgcacca gcacggtcac gccgttgtcg cgcgcgaagt cgaccaccag
     2221 cccggagaag cccttcacct cgacgttgcg cagctccttc accacctcgc ggatcagcgc
     2281 cacgcgctcg tccatgttga agcgcgggtt cttggcgatg ttggcgccca cggcgacgat
     2341 cagccgcggg aacatggcgg cggcgcgctg gatgatgtcg tagtggccga gggttatcgg
     2401 atcgaaagtg ccgctgtacg ccgccaccac gctcatgttt tctccatgcg gtaactggcc
     2461 agtccgtacg acacctggcc ggcctgcttc tcgcgcagca gttcgtagcc tgggggcaga
     2521 ggcggcgcgc caccgcgcca ttccaggtac acgcggttgt cgggcagcag gcggctgggc
     2581 agacgctcca gtgcgcggcc gagcaggtcg gactcgtacg gcggatcgag aaacaccacg
     2641 ttgtagcgcg tggcggtgat ttccaggtac gacagcgcat ccatcggtcg catgtcgaaa
     2701 cggtgatcgg ccttgaactg cagcagcgcg gcgtggatca gcgagatctg cgtgcggccg
     2761 ttttcgacga aggtgacttt acgcgcgccg cgcgacagcg cctcgatgcc cagcgcgccg
     2821 ctgccggcga acaggtcgag gcagcgcgtg ccctcgatca gcggtgccag ccagtcgaac
     2881 acggtctggc gcacgcggtc cggcgtgggc cgcacgccgc tgtcgccgtc gaactcgacc
     2941 agccggctgc gccactctcc accgatgacg cgcaatttcc ccggagcacg cttcatggcg
     3001 catgagttaa cgcctgccgc cgtttgcggt caagcggcgc ggcgcaaaaa ttttttccgc
     3061 agccgccggt gtcgatgtgc gaacaacgcg cggatgaccg gccgcagcaa tggcgccatg
     3121 aagaacaacc tcggctcgaa ttccagttca tcggtgatgc gcgttacctc gccgtgctgc
     3181 tcgatgcggc gacgatggat ccacacgcgc tgcagccacg accatgaacg ctcgtcgaag
     3241 cccacgcccg ggtacaggcg ttcgagcatc aggtgatgac gatccgccgg cagcaggccg
     3301 aacagcagca gcgtgctgac gaaggcggtt tggcccagct tgagcagctg tgatgagagg
     3361 tcagtggcat cgcgtggatg cgtcatgcgc atcagcggca gcagttcgtg gttgacgccg
     3421 gccatcgacg aggcgtggcg ccagacatcg gccggcgccg cgtcgagatc gctgctgaaa
     3481 cggagctgga tcatgcgcag cagtttatcg ccgcatgcat gccgcacggt ttcccagtag
     3541 ccttgcgcca tggccgccaa atccgaaaac gtcagcccca ccgcctacgc caccggctac
     3601 ctctggtatc gcaacggcat gtcgcacgag gcgctcgcca ccgagcaggg gcgcaaggtc
     3661 gaccgctggt tccatggctt catcgccgcc acgcgtgcgc tcaccggcgt gcagctcgac
     3721 accatgctga tcgcgcggca caagggcatc gacggcgaac tggcgcgcgc catcgaggcg
     3781 gggcgcgtcg gccaggtggt ggagatcgcc gccggcctgt cgccgcgcgg caccaccttc
     3841 tgccagcgct acccgaagct cacctacatc gagaccgacc tgccgaagat ggccgcgctc
     3901 aagcgcagcc tgctggacaa gggcgggttg ctgtcggcgc gccaccgcgt gcaggtggtc
     3961 gatgcgctgg cgcagcgcgg cgccaattcg ctggccgcgc tggccaaaac gctcgatgcg
     4021 cagcagggcg tcgccgtcat caccgaaggg ctgatgagtt atctcgaccc ggtggctgcg
     4081 caccacgtgt ggcgcaacac tgcgcatttc tgcaagcgct tcccgcatgg cctttacctc
     4141 tccgacgcct acctcgccgg agatcgcagc ggcgcggcct tcgccgcctt cggcctgatg
     4201 atctccgcct tcgtgcgcgg gcgcttgcat cgccagttcc gctctgccga gcacgcgcgc
     4261 gagcggctgg ccagggccgg ttttaccgac gtgcgcatgc tgcagacgtc tgagattcct
     4321 gaaacgcgag agctgtcgaa gttgcgcggg ccggatcgcg tgcgcgtgct ggaagcaagc
     4381 acataagcgg cagacgcgcg gttttccgat tgacaaagcg gcgcagcgat ctccagcatc
     4441 cggtcctgtg ggtgtcgaca gggagcgttg caatgtctat cagacttggc tgctcggccc
     4501 tggtgatgtt ttttgcgccg gctatttacg cgcaggacgc cactactccg accgcgccgc
     4561 cgccacagtc cgccgaatct cccacggtgc tcgacacggt gcaggtgacc ggcacgcggc
     4621 tggctcgccc tggatactcc acgccaacgc cgacaacatc agtcacggcg cgcgacatac
     4681 gcgactccgg caccacaaac ttgggcgact acctcgataa cctgccccag ttgtcgagct
     4741 cggccaacat ggccaactcg acgcagttca tcggcaccgc cggtcttaac gaactcgacc
     4801 tgcgcgatct cggcacctcg cgcacactgg ttctggtcaa cggccaccgc catgtcggcg
     4861 cgctgggcgg cgacacagcg gtggacatca acaccattcc gcaggacctg gtcgaacgcg
     4921 ttgacttcat taccggtggc gcttctgcaa tctacggtgc cgatgcagtg accggcgtcg
     4981 tgaacttcgt gctgacgcag aatttcgaag gcctgtcggt gcgtgcgcag accgggacca
     5041 actgggacgg cggttacgac aagaatctcg tcgccattac tgcagggacc aattttgctg
     5101 gcgaccgtgg caacttcgtc ctcgcaggcg aattcgccca gcagtcgcgc cttacggcaa
     5161 aggagcgtcc gagcatggct ctgcagcgca gctatctgca gaacccgcta gacgtcgacg
     5221 gcaacggcaa cccgatcgag aaacccaatc gcaggcccaa catgattctc gtgcccggcg
     5281 ctggcaccaa cgagaccagc gaaggcggca ccgtattaac cgccggttat atcaacgacg
     5341 gcaatattcg cgacgcttac ctgtttgatc ccggtggcaa cttccgccgc gtgcgcatcg
     5401 atggaatagt cgatgccggc aacgagcttt gctccgattg cgattacctg gacgaaaatg
     5461 cctaccagga tctgcaaccc gccttcgatc gctacaacct gaccgcgatg tcgcacttcc
     5521 agatcagcga ctgggcgcgc ctggatgtcg aaggcaattt cgcgcatacc cgcgcattga
     5581 cctacttctt cccgtccttc gatgacgtat tccagggcaa cgccatcgca atacaaccag
     5641 acaatgcgta catgcccgcg ggcctgcagg cattctttac cagccacaat aaaagggagg
     5701 ttatggtcaa tcgtatcgat gccgacgccg ggcttcgcgc cgagaaggac gagcgcgaca
     5761 tgacccaagt gacgctggcg ctcactggta accttccctt ttcgccgacc tggaactaca
     5821 acctgtcggc cgtgtacgga cacacccgca tcgacaccac gttcatcaac aaccgaatca
     5881 atgatcgctt cagtgaggca accgatgcag tggttgaccc gatgacccac aacatcgtct
     5941 gccgagccac catcgattcg aaagccaagc tgcctgggac caataccccg gtgccggccg
     6001 acgcgcgcgt cggctgtatt ccgaccaatg tgtttggcgc cggtgcgatc aaccaggcct
     6061 cggccaattg gttcaacgcc accgggctgg aaaccgacgg tgtcagcctc gccgtctata
     6121 cggcctacct ggtgaatgat gcgctgatcg atttgcccgc cggaccgctc gccgccgcgt
     6181 taggcacgga atacaggttg gagcaatcga atacgttgcc ggatgcactc tccgccaccg
     6241 gcgcaacctt caacaacata ctgcttccgt tgtcgggagg ctttcacgcc tgggacggct
     6301 tcgctgaaac ctcggtgccg ctgatcggcg gaatccccct agtaaaactg ctggaactgg
     6361 aactcgcagc gcggtactcg agctacaaca cgatcggcag cacgctgtcg agcaaggtcg
     6421 gcctgaactg gacggtagcg ccggacctgc gcatccgctc gacctttgca cgtgccgtac
     6481 gcgcgcccaa tatcggcgaa ctctattctc cggccagcca ggatttcttt ttcgtcaatg
     6541 acccgtgctc gcaaaagaac gtgaagaacg cacccgatcc ggccatacgc agcaataact
     6601 gtgcagctct caacatcccc gcggactttc actcgcatgc cgatgccgac acaattcctg
     6661 gcgtgtccgg cggcaatcct gatctgacgg aagaacgtgc gaccacctgg actgcaggtg
     6721 cggtcctgac gcctcgctgg atcaccggct ggtcggtcag cgtcgatttc tgggacatct
     6781 cgatcaacaa cgcgatcgat ctgctcgacg cgcaggacat cctcaacaaa tgtgtcgatg
     6841 atgccggcgg cccgaacacg cagttctgcg cgctggtcac gcgcgatccg acgtcgcacg
     6901 aaatcaccaa catcgtcgac atcgaggaaa atgttcagcg cctgaccgca cgcggcgtcg
     6961 acttcgagtc cagctataaa ttcccaattg ctggcggcag caccgagttc cacctggtcg
     7021 gtacctatct caaccagttg cgcacctatc cattccagag cgaacccaag cgctcgtttg
     7081 aggcggctgg cgtgctgggc gatcctcgct ggcagatcaa tctcgacacg cgctatgacc
     7141 atggtccgct gagtttcacg tggagcattc gctggatcga taagcaatat ctggtcgacc
     7201 gcgatacgtt gcaggctcac cccgacgcgc aggaaccgat acgcgcgggc acgcgcttct
     7261 accacgacgt gcaggcgcgc tatcacctgc ctggctcgat gaaggcagtg gaggtgtacg
     7321 caggcgtcga caacctgctc aatgctgata tcccgacggc aggatcgctg ctgggcgtgg
     7381 gcaccggcag cgccatctac gacaacgtcg gccgcttcct ctacgctggc gtggaataca
     7441 agatctagtg cggcaacaac gcggcacccg acttgatgga ggccagactt gaatcacgcc
     7501 atctggagcg tcgactggca cgcgctgttc gtgccgaccg gctcgctgct ggaaatcttc
     7561 atccgcggca gcgtcatcta cctgctactg tttttcctga tgcgcttcct gcgccgcgag
     7621 gcggggcaga tcggcatcgc cgacgtgctg gtcatcgtgg tcatcgccga tgccgcgcag
     7681 aacggcatgg ccggcaccta tacctcgatc accgaaggcg tggtgctgat cgccaccatc
     7741 atgggctggg actacctgct cgactggctc gacttccact ggcccgcgtt cgcacgcctg
     7801 gtgcacccgg cgccggtgcc gctggtggtc gatggccgcg tgctgtggaa gaacctgcgg
     7861 cgccagctga tcacggaaag cgaactgcgc tcacgcatgc gcgaggaagg catcgagcac
     7921 ctggagagcg tgaagcgctg ctacatggaa ggcgatggcc gcatcagcgt gatcggccat
     7981 ggtggaggcg cgcgctgagg gtgcaaaaaa ccttccaccg caccggcaat tttgatgtta
     8041 ataattattg tcttaaagta attacagaca acaatttata acaaccagaa agcgggtgcg
     8101 gtgcaggcag aaaactcgat tacttcgcct tcgcctggtt ggccactgct tcggcggctt
     8161 tcttcgccgc ttccggatcg cccaggtagc ggtagctctt caccttgagg ttgtcgtaca
     8221 gctcgaacag cagcgggatg ccggtgggaa tgttgagctc gacgatggct tcgtcggaaa
     8281 tgttgttcag gtacttgtac agcgcgcgca gcgagttgcc gtgcgcggtg accagcacgg
     8341 tctggccgct cttgagctgc ggggcgatgg cgtcatgcca gtacggcagc acgcgctcca
     8401 gcgtggtctt gagcgattcg gtggcgggca gtgcgctctg gtcgagcttg ccgtagcggc
     8461 ggtcaaagcg cggatgctgc ggattgtcct cggccatcgg cggtggcgga atgtcgtaac
     8521 tgcggcgcca gatcttcacc tgtgcctcgc catatttggc ggtggtctcg gccttgtcca
     8581 ggccctgcag cgcgccgtag tggcgctcat tgaggcgcca ggatttctgc accggcagcc
     8641 acagctggtc catctcggtc agcgcgacca tcagcgtgtt gatggcgcgc ttgagcaccg
     8701 aggtgtgcgc cacgtcgaac atcaggcctt cctcgcgcat cagcttgc
//
LOCUS       DASZZC010000004         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_450078,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000004 DASZZC010000000
VERSION     DASZZC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450078"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VHE37_00085"
     CDS             <1..526
                     /locus_tag="VHE37_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HVT33949.1"
                     /translation="GILLAKDLLKFTSTVPGFDSATFDLGRWLRPVVFVPESKRLNVL
                     LKDFRRGRNHMAIVVDEYGGVAGLVTIEDVLEQIVGEIADEYDEAESARILKQDDRRY
                     LVNALTTIEEFNKYFHAEFPTGEFDTVGGLVTHRFGRMPKRGESVRIERFNFNVQRAD
                     SRRVQQLQVTIAPG"
     gene            complement(523..660)
                     /locus_tag="VHE37_00090"
     CDS             complement(523..660)
                     /locus_tag="VHE37_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33950.1"
                     /translation="MTTPAALENDCTAPASGMLLIIVSLTKQYIFYNITTGGAVDAFS
                     P"
     gene            659..>1951
                     /gene="lnt"
                     /locus_tag="VHE37_00095"
     CDS             659..>1951
                     /gene="lnt"
                     /locus_tag="VHE37_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HVT33951.1"
                     /translation="MRRWELLLAPLCGVLLTLALAPFDLKPLAPVALALFFWLQERST
                     HWRRAALLAWLMLSAHFVSGVYWIYISTHIYGGAAAWLGVLLCVALLTVYMPLFPVPL
                     FALLRWRALPAWAGWMAMPAVFTLGEMMRGHVLWKGFAWLCLGDAALDTPLARLVPIV
                     GVHGITLVIALSGYALYRLVADRRLSARVRALMILVAPPALCLLLPAGGSWTQDSGAP
                     LTAAIVQPDVLQEDKWQEDMQMPILQRHLDLTRSVLGADLIVWPEVSLTQPYHLLAPY
                     VLPLLDREARAANSALIFGVVIRDDDSGGYYNSVLALGAASGRYNKRHLVPFGEYFPI
                     PDFIRPLLDVLGTPYSDFLVGREDQPPLAAKNQQLGIYICFEDVFGNEFRTRARGTTL
                     SVSVTNDAWFGHSGAAAQHLAMGRMRSLETGRMTLRASN"
BASE COUNT          298 a          654 c          647 g          352 t
ORIGIN      
        1 cggcatcctg ctggccaagg acctgctcaa gttcacctcc accgtgcccg gcttcgattc
       61 agccacgttc gacctgggcc gctggctgcg cccggtggtg ttcgtgccgg agtccaagcg
      121 actcaacgtg ctgctgaagg atttccgccg cggccgcaac cacatggcga tcgtagtcga
      181 cgaatacggc ggcgtggccg gcctggtcac catcgaggac gtgctggagc agatcgtcgg
      241 cgagatcgcc gacgaatacg acgaggccga gagcgcgcgc atcctcaagc aggacgaccg
      301 ccgctacctg gtcaacgcgc tcaccaccat cgaggaattc aacaagtatt tccatgcgga
      361 atttcccacc ggcgaattcg acaccgtggg cgggctggtc acgcaccgct tcggccgcat
      421 gcccaagcgc ggcgagagcg tgcgcatcga gcgcttcaac ttcaacgtgc agcgcgccga
      481 cagccgccgc gtgcagcagc tgcaggtgac cattgcgccg ggctagggcg aaaaagcgtc
      541 caccgcaccg cctgtggtga tgttataaaa aatatattgt tttgttaaag atacaataat
      601 taataacatg cccgaggccg gtgcggtgca gtcattttcc agcgcggcgg gcgtggtcat
      661 gcgccgctgg gagctgctgc tcgcgccgct ctgcggcgtc ttgctcacgc tggcgctcgc
      721 gccgttcgat ctgaagccgc tggcgccggt cgcgctggcg ctgttcttct ggctgcagga
      781 acgcagcaca cactggcgtc gcgcggcctt gctcgcatgg ctgatgctgt cggcgcattt
      841 cgtcagcggc gtctactgga tctacatcag cacgcatatc tacggcggcg cggcggcgtg
      901 gctcggcgtg ctgctgtgcg tggcgctgct caccgtgtac atgccgctgt tcccggtgcc
      961 gctgttcgca ttgctgcgct ggcgtgcgct gcccgcatgg gcggggtgga tggccatgcc
     1021 ggcggtcttc acgctgggcg agatgatgcg cggccatgtg ctgtggaaag gcttcgcctg
     1081 gctgtgcctg ggcgacgccg cgctcgatac gccgctggcg cggctggtgc ccatagtcgg
     1141 cgtgcatggc atcacgctgg tgatcgcgct gtctggctac gcgctgtacc ggctggtggc
     1201 ggaccgccgc ctcagcgcgc gcgtgcgtgc cttgatgatc ttggtcgcgc cgcccgcgct
     1261 gtgcctgctg ctgcccgccg gcggcagctg gacgcaggac agcggcgcac cgctcaccgc
     1321 cgccatcgtg cagcccgacg tgctgcagga agacaagtgg caggaagaca tgcagatgcc
     1381 gatcctgcag cggcacctgg acctgacccg ctccgtgctc ggcgccgatc tcatcgtgtg
     1441 gccggaagtg tcgctgaccc agccctacca cctgctggcg ccgtacgtgc tgccgctgct
     1501 cgaccgcgag gcgcgcgccg ccaacagcgc tctgatcttc ggcgtggtga tccgcgacga
     1561 cgacagcggc ggctactaca acagcgtgct ggcgctgggc gccgcgtcgg gacgctacaa
     1621 caagcgacac ctggtgccgt tcggcgaata ttttccgatc ccggatttca tccgaccgct
     1681 gctggacgtg ctcggcacgc cgtactccga tttcctggtc ggccgcgagg accagccgcc
     1741 gctcgccgcc aagaaccagc agctgggcat ctacatctgc ttcgaggatg tgttcggcaa
     1801 cgagttccgc acccgcgcgc gcggcaccac gctgtcggtg agcgtgacca acgacgcctg
     1861 gttcggccat tccggcgcgg ccgcacagca tctggcgatg gggcgcatgc gctcgctgga
     1921 gaccgggcgc atgacgctgc gcgcgtccaa c
//
LOCUS       DASZZC010000005         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_707184,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000005 DASZZC010000000
VERSION     DASZZC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707184"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1571
                     /locus_tag="VHE37_00100"
     CDS             207..1571
                     /locus_tag="VHE37_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVT33952.1"
                     /translation="MRQQQRIAVIGAGPSGIAAAKECIAHGLGDQLVVFEKSDQVGGN
                     WVYREGAGHSSVYSTTHIISSKYYSEYEDFPFPAGTPDYPHHTHLKKYFQDYAAHFGV
                     TQKIRFHTEVLHAARGSDGKWTLRVRGAAGESTETFDVLMVANGHHWNPRLPEYPGSF
                     DGEFIHSHAFKNNEPYRGKRVLVIGGGNSAADVAVECSRVAQRCVISMRRGHWFLPKF
                     MFGLPGDVIYEKAAWAPRWFRQWALRTSVNVIAGRNERFGLQRPEYDILEGHPTLNSE
                     LMYFLGHGEIIPKPGVAKLDGKRVQFADGSSDTFDVIIAATGYKITFPFFDRSFIDFE
                     DAVSVPLWRRMFHADISNLYFIGLFQPLGCIWPLADYQALLACLELTGKYRRPPDMKA
                     AIADEQAHPHFNFVKTPRHSTEVDYADFRKELFAELRKAGVQPHARPANAAIYQTEAW
                     QKYA"
BASE COUNT          303 a          538 c          482 g          265 t
ORIGIN      
        1 tggatgccaa cggccacatc tcgctggcgt ggcagagcta cggccaggat ggcagcggcc
       61 gcggcatcta cgcccgcgcg cagtaaacct tcttcccttt cccgccagca cgctcaggca
      121 cgacctgagc ctccacaaaa cttgacgcat gcgtcatctt tttgcatgat gccggcatgc
      181 cagcaacagc gcgtggagaa atttgcatga gacagcagca gcggatcgca gtgatcggcg
      241 cgggcccttc cggcatcgcc gccgccaagg aatgcatcgc gcatggcctg ggcgaccagc
      301 tggtggtgtt cgagaagagc gatcaggtcg gcggcaactg ggtgtaccgc gagggcgccg
      361 gccactcgag cgtgtactcg accacgcaca tcatcagttc caagtactac tccgagtacg
      421 aggacttccc cttccccgcc ggcacgccgg actacccgca ccacacgcac ctgaaaaaat
      481 atttccagga ttacgccgcg catttcggcg tcacgcagaa gatccgtttc cacaccgagg
      541 tgctccacgc cgcgcggggc agcgatggca aatggaccct gcgcgtgcgc ggggccgccg
      601 gcgaaagcac cgagacgttc gatgtgctga tggtggccaa cggccatcac tggaatccgc
      661 gactgccgga atatcccggc agcttcgacg gcgagttcat ccacagccac gccttcaaga
      721 acaacgagcc gtatcgtggc aagcgcgtgc tggtgatcgg cggcggcaac agcgcggccg
      781 acgtcgccgt ggaatgctcg cgcgtcgcgc agcgctgcgt catctccatg cgccgcggac
      841 actggttcct gcccaagttc atgttcgggc tgccgggcga cgtaatctac gagaaagccg
      901 cctgggcgcc gcgctggttc cgccagtggg cgctgcgcac ttcggtgaac gtgatcgcgg
      961 gcaggaacga acgcttcggc ctgcagcggc ccgaatacga catcctcgaa ggccacccga
     1021 cgctgaactc ggaactgatg tatttcctcg gccacggcga gatcatcccc aagcccggcg
     1081 tggcgaagct cgacggcaag cgcgtgcaat tcgccgacgg cagcagcgac actttcgacg
     1141 tgatcatcgc cgccaccggc tacaagatca ctttcccgtt cttcgacagg tcgttcatcg
     1201 atttcgagga cgccgtcagc gtgccgctgt ggcggcgcat gttccacgcg gatatttcca
     1261 acctgtactt catcggcctg ttccagccgc tgggctgcat ctggccgctg gccgactacc
     1321 aggccttgct ggcctgcctg gaactgaccg gcaaataccg tcgcccgccg gacatgaagg
     1381 ccgcgattgc cgacgagcag gcgcatccgc acttcaactt cgtcaagacg ccgcgccact
     1441 cgaccgaggt ggactacgcc gatttccgca aggaactttt cgcggagctg cggaaggccg
     1501 gcgtgcagcc gcatgcccgc ccggcgaacg cggccatcta ccagaccgaa gcgtggcaaa
     1561 agtacgcgtg accccgtagg cttgcccg
//
LOCUS       DASZZC010000006         5922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_707202,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000006 DASZZC010000000
VERSION     DASZZC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707202"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VHE37_00105"
     CDS             complement(<1..949)
                     /locus_tag="VHE37_00105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HVT33953.1"
                     /translation="MRGIRGRWVMGKTLYDKLWDAHVVRDNGDGSSLIYIDRQLLHEV
                     TSPQAFDGLRLNGRQPWRIAANLAVADHNVPTTDRSGGIADPISRTQVDALDENCAQY
                     GITEFRMNDLRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEH
                     ALATQTLVLKKSKNMRVTVRGQVSPGITAKDIVLAVIGRIGTAGGTGYTIEFAGEAIR
                     ALSMEGRMTVCNMAIEAGARAGLVAVDDTTIAYVQDRPYSPRGENLQKAIAWWRTLVS
                     DADAKFDHEVAIEAAEIQPQVTWGTSPEMVLPVSARVPDP"
     gene            1019..1891
                     /locus_tag="VHE37_00110"
     CDS             1019..1891
                     /locus_tag="VHE37_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT33954.1"
                     /translation="MDTQTLAAFAEVADSASFSRAAEKLHLSQPAISKRIAALEAQLG
                     KPLFDRVGRAIHLTDAGRTLLPYARRTLQHIEDGRRALTQLAGEVGGRLSFGTSHHIG
                     LHRLPPVLRRYTQKYPQVDLDIHFMDSEVACEEVLAGRLELGIVTLPLQPLPQLQTRV
                     VWPDPLAVMVAADHPLAHKRKVKLQQLAEFPAVLPDAQTYTHRIVRRELEKHGLSPRI
                     RLATNYLETLKMLVAINLGWSVLPRSMLDRGVRELHIAELAMQRDLGLVWHEQRTLSG
                     AALALRQLLPPAKS"
     gene            1960..3006
                     /locus_tag="VHE37_00115"
     CDS             1960..3006
                     /locus_tag="VHE37_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33955.1"
                     /translation="MGVRATLLLPMLLLGGCGLLPHRLDAPPQPPPATPQPAAAEVPD
                     PCASLGERNLIDDTKRRLQETVCQTALWFDGFFGERDVRDISAARESSGRAELSTAYS
                     QFTGTKVGLRFNAHMTLPNLRHRLSVFVGRDSEDSFVRDRSESYALRTRFPHLEDHDE
                     TIAGLGYALPESYRFKSEFRTGVRFAGLTVPKAFAQFRFAYNAYADDLNLVDLRTTPF
                     INTRDGLGLTTTLDYSRVLGDSRLFRWSNIATVTQKAWDSGVDWRSAAIIYQGLTGKR
                     AVALETFVRGDTGAPVPLAEYGSQVIYRQPLAHERLYGEAVTGYSWPKTDPNAQRKGS
                     YAVALILELPFGEP"
     gene            complement(3012..3797)
                     /gene="ygiD"
                     /locus_tag="VHE37_00120"
     CDS             complement(3012..3797)
                     /gene="ygiD"
                     /locus_tag="VHE37_00120"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HVT33956.1"
                     /translation="MNAPTPAMPAVFFAHGTPMNAIQHNGYTQAWRDIAAAMPRPRAI
                     LAISAHWYTHGTWVHASERPRTIHDFGGFPRELFQVQYAAPGSPALARRVRELLAPLP
                     VGLDTEWGYDHGSWSVLVHAYPQADIPVVQLSMDATQPLQHHYDMGRRLAALRDEGVL
                     IAGFGNIVHNLRAIEPGGAPADWAVRFERGVRERIERGEHESLIDYPKLGADARMSVP
                     TPEHYLPLLYVLGAQRGDDRVSFHCDQVDLGSIGMLTVVLAPR"
     gene            3993..5549
                     /locus_tag="VHE37_00125"
     CDS             3993..5549
                     /locus_tag="VHE37_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVT33957.1"
                     /translation="MSDSDLPRDALAQLEARKERAREMGGADKIERQHARGALTARER
                     IARLLDADSFFELGQLNHSDVPGMEARTAADGKIAGYGRIDGRSVAVAADDVTVLAGS
                     GGRIGGRKSHRILELAVEKGYPLINLGEAGGARIPDILGSDGLSSMTIGMDAAQRCRR
                     VPMVAAIFGECFGSASWYAALADFVVQVKGSCMAVSGPRVLEMATGEKSSNDELGGWE
                     LHARVTGLADRAAEDEAHALAIVREFLSYLPSSEAQLPPVYQGDDEAAARQSRLDTFL
                     PDSPRHGYDMHEIIHTLADHRHFFPVKPEFDRSVITGFMRVDGRSVGVIASNPRHAAG
                     AMGPDGCDKCASFIALCDSFHVPLLFLHDTPGFFVGKAAEQKRMPGKIINFIEALSLA
                     TVPIVSIVVRKSYGMAYGNMAGSGMGADFVYAWPTADIGFMAPEVAANVVGGPKIKSA
                     PDPKAAMDQVIAEIRESTAPWRAAGLHYLDDVIRPSDTRAVLIRSMELARGNNGGRSQ
                     RRLANWPTSF"
     gene            complement(5546..>5922)
                     /locus_tag="VHE37_00130"
     CDS             complement(5546..>5922)
                     /locus_tag="VHE37_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT33958.1"
                     /translation="KLIPQVGARPLMLAALGATALRWSITALAVDSWPVLVVAQLSHA
                     LTFGAYHAVAMHYVTRLFPARLQGRGQAVYNAVAYGLGGSIGSLSAGYLWEALSPQSV
                     FLLAGLVAALGFWVGWRKLPPA"
BASE COUNT          981 a         2058 c         1947 g          936 t
ORIGIN      
        1 caggatcggg cacgcgcgcg gacaccggca gcaccatctc gggcgacgtg ccccaggtga
       61 cctgcggctg gatctccgcg gcttcgatgg cgacttcgtg gtcgaacttc gcatcggcat
      121 cgctcaccag cgtgcgccac caggcgatgg ccttctgcag gttctcgccg cgcggcgaat
      181 acggccggtc ctgcacgtag gcaatggtgg tgtcgtccac cgccaccagg cccgcgcgcg
      241 cgccggcctc gatggccatg ttgcacacgg tcatgcggcc ttccatcgac aacgcgcgga
      301 tcgcctcacc ggcgaattca atcgtgtatc cggtgccgcc ggcggtgccg atgcggccga
      361 tcaccgccag cacgatgtcc ttggcggtga tgccgggcga gacctgcccg cgcacggtca
      421 cgcgcatgtt cttcgacttc ttcagcacca atgtctgcgt ggccagcgcg tgctcgacct
      481 ccgaggtgcc gatgccgaag gccagcgcac cgaacgcgcc gtgcgtggag gtgtgcgaat
      541 cgccgcagac caccgtcatg cccggcagcg tggcgccctg ctccgggccg atcacgtgca
      601 cgatgccctg gcgcagatcg ttcatgcgga attcggtgat cccgtactgc gcgcagtttt
      661 catcaagcgc atcgacctgc gtgcgcgaga tggggtcggc gataccgccg ctgcggtcgg
      721 tggtcggcac gttgtggtcg gccactgcga gattggcggc gatgcgccac ggctggcggc
      781 cgttcaggcg caggccgtcg aacgcctgcg ggctggtcac ctcgtgcagc agctggcggt
      841 cgatataaat gagcgacgag ccatcgccgt tgtcgcgcac cacgtgcgca tcccacagct
      901 tgtcgtaaag cgtcttgccc atgacccacc ttcctcgaat gccgcgcatg ctagtcccgc
      961 gtaatcaata actccaatga atcgttttca taagcagcat tcctgattag aatgaatcat
     1021 ggacacccag accctcgccg cctttgccga agtcgccgac agcgcctcat tctcgcgcgc
     1081 ggcggaaaag ctgcacctgt cgcagcccgc catctccaag cgcatcgccg cgctggaggc
     1141 gcagctgggc aagccgctgt tcgaccgcgt cggtcgcgcc atccatctca ccgacgccgg
     1201 ccgcacgctg ctgccctacg cgcggcgcac gctgcagcac atcgaggatg gccggcgcgc
     1261 gctgacccag ctggcgggcg aagtcggcgg ccgcctgtcg ttcggcacca gccaccacat
     1321 cggcctgcat cgtctgccgc cggtgttgcg ccgctacacg cagaaatatc cgcaggtgga
     1381 tctcgacatc cacttcatgg actcggaagt ggcctgcgag gaagtgctgg ccggccggct
     1441 cgaactcggc atcgtcaccc tgccgctgca gcccttgccg caattgcaga cgcgcgtggt
     1501 gtggcccgat ccgctggcgg tgatggtcgc cgccgatcac ccgctggcac acaagcgcaa
     1561 ggtgaagctg cagcagctgg cggagtttcc cgccgtgctg ccggacgcgc agacctacac
     1621 gcaccgcatc gtgcgccgcg agctggagaa gcacgggctg tcgccgcgca tccgcctggc
     1681 caccaattac ctggagacgc tgaagatgct ggtggcgatc aacctgggct ggagcgtgct
     1741 gccgcgctcc atgctcgacc gcggcgtgcg cgagctgcat atcgccgagc tggcgatgca
     1801 acgcgacctg ggcctggtat ggcacgagca acgcacgctg tccggcgcgg cactggcgct
     1861 gcggcagctg ctgccgccgg cgaaatcctg atgcgcgcaa tacgctaggc ttgcagcggg
     1921 cgcgatcgac cccaacaaca agcgcgccgg agggagtgca tgggagtgcg ggcgacgctg
     1981 ctgttgccga tgctgctgct gggcggctgc ggactgctgc cgcatcggct cgacgcaccc
     2041 ccgcaacccc cgccggccac gccgcaaccg gccgctgccg aggtgcccga cccgtgcgcg
     2101 tcgctgggcg agcggaattt gatcgacgac accaagcgcc gcctgcagga aaccgtgtgc
     2161 cagaccgcgc tgtggttcga cggctttttc ggcgagcgcg acgtgcgcga catcagcgcc
     2221 gcgcgtgaat cctccggccg cgccgagctg tccaccgcct actcgcagtt caccgggacc
     2281 aaggtcggcc tgcgcttcaa cgcgcacatg accttgccca acctgcgtca ccgcctgtcg
     2341 gtgttcgtcg gccgcgacag cgaggatagt ttcgtgcgcg accgctcgga gagctatgcg
     2401 ctgcgcacgc gtttcccgca cctggaagac cacgacgaga ccatcgccgg cctgggctac
     2461 gcgctgccgg aaagctatcg cttcaaatcc gagttccgca ccggcgtgcg cttcgccggc
     2521 ctcaccgtgc ccaaggcctt cgcccagttc cgcttcgcct acaacgccta cgccgacgac
     2581 ttgaacctgg tggacctgcg caccacaccg ttcatcaaca cacgcgacgg actgggcctg
     2641 accaccacgc tggactacag ccgcgtgctc ggcgattcgc gcctgttccg ctggagcaac
     2701 atcgccacgg tgacgcagaa agcctgggac agcggcgtgg attggcgcag cgccgccatc
     2761 atctaccagg gcctgaccgg gaagcgcgcg gtggcgctgg agacgttcgt gcgcggcgat
     2821 accggcgccc cggtgccgct ggcggaatac ggcagccagg tcatctaccg ccagccgctg
     2881 gcgcacgagc gcctgtacgg cgaagcggtc accggctaca gctggccgaa gaccgacccg
     2941 aacgcgcagc gcaagggctc ctacgcggtg gcgctgatcc tcgaactgcc cttcggcgag
     3001 ccgtagcgcg atcagcgcgg cgccaacacc accgtcagca tgccgatcga accgaggtcg
     3061 acctggtcgc agtggaacga cacgcggtca tctccgcgct gcgcgccgag cacgtagagc
     3121 agcggcaggt aatgctcggg cgtgggcacc gacatccgcg cgtcggcgcc gagcttcggg
     3181 taatcgatca gcgattcatg ctcgccgcgc tcgatgcgct cgcgcacccc gcgctcgaag
     3241 cgcaccgccc aatccgccgg cgcaccgccg ggctcgatgg cgcgcaggtt gtgcacgatg
     3301 ttgccgaagc cggcgatgag cacgccctcg tcgcgcagcg cggcgagcct gcggcccatg
     3361 tcgtagtgat gttgcagcgg ctgcgtggcg tccatcgaca gctgcaccac cggaatgtcc
     3421 gcctgtggat acgcgtgcac cagcaccgac cagctgccgt ggtcatagcc ccactcggtg
     3481 tcgagcccca ccggcagcgg cgccagcaat tcgcgcacgc gccgcgccag cgccggcgat
     3541 cccggcgccg cgtactgcac ctggaacagc tcgcgcggaa atccgccgaa gtcgtggatg
     3601 gtgcgcggcc gctcagacgc atgcacccag gtgccgtgcg tgtaccagtg cgcggaaatg
     3661 gcgaggatcg cgcgcggccg cggcatggcc gctgcgatat cgcgccaggc ctgcgtgtag
     3721 ccgttgtgct gaatggcgtt catcggcgtg ccgtgggcga agaacacggc gggcatggcg
     3781 ggcgtcggtg cgttcatggg gttttggaca gtgtcagcga aaaaatgatt tcatggcggc
     3841 gcatgcagcg cgcattttca gcctcaccgc gttgccattc tgcctgttat taattgtata
     3901 atgtttttaa tttaatacta tttttaataa catcgcaaag gccggtgcgg tgcaacgatt
     3961 ttcagccgtt ttttttctgc gaggacgaac acgtgtccga tagcgatctg ccgcgcgacg
     4021 cgctggcgca actcgaagcg cgcaaggagc gcgcgcggga aatgggcggc gccgacaaga
     4081 tcgagcgcca gcatgcgcgt ggtgcgctca ccgcgcgcga gcgcatcgcg cgcctgctcg
     4141 acgcggactc gttcttcgag ctgggccagc taaatcattc cgacgtgccg ggcatggaag
     4201 cgcgcaccgc ggccgacggc aagatcgcgg gctacggccg catcgacggg cgcagcgtgg
     4261 cggtggcggc agacgacgtc accgtgctcg ccggctcggg cggacgcatc ggcgggcgca
     4321 agtcgcaccg catcctcgaa ctcgcggtgg agaaaggcta tccgctgatc aacctgggcg
     4381 aagccggcgg cgcgcgcatc ccggacatcc tcggttccga cggactctcc tcgatgacca
     4441 tcggcatgga cgccgcgcag cgctgccgcc gcgtgcccat ggtggcggcg atcttcggcg
     4501 aatgcttcgg ctccgcgagc tggtacgcgg cgctggcgga tttcgtggtg caggtcaaag
     4561 gctcgtgcat ggccgtgtcc ggcccgcgcg tgctggagat ggccaccggc gagaaatcca
     4621 gcaacgatga actcggcggc tgggaactgc acgcgcgcgt caccggcctg gccgaccgcg
     4681 cggcggaaga tgaggcgcat gcgctggcca tcgtccgcga attcctctct tacctgccct
     4741 cgtcggaagc gcagctgccg cccgtctacc agggtgacga cgaagcggct gcgcgccagt
     4801 cgcgcctcga cacgtttctc cccgactcgc cgcggcatgg ctacgacatg cacgagatca
     4861 tccacacgct ggccgatcac cggcatttct tcccggtgaa acccgagttc gaccgcagcg
     4921 tgatcaccgg cttcatgcgc gttgacggcc gcagcgtggg cgtcatcgcc agcaacccgc
     4981 gtcacgccgc cggcgccatg ggcccggacg gctgcgacaa gtgcgccagc ttcatcgcgc
     5041 tgtgcgattc gttccacgtg ccgctgctgt tcctgcacga cacgccgggc ttcttcgtcg
     5101 gcaaggccgc ggagcagaaa cgcatgccgg gcaagatcat caacttcatc gaggcgctgt
     5161 cgctggccac ggtgccgatt gtctccatcg tcgtgcgcaa gtcctatggc atggcctacg
     5221 gcaacatggc cggcagcggc atgggcgcgg atttcgtcta cgcctggccg acggcggaca
     5281 tcggcttcat ggcgccggaa gtggcggcga acgtggtcgg cgggccgaaa ataaaatccg
     5341 cgcccgatcc aaaggcggcg atggaccagg tgatcgccga gatccgcgaa agcaccgcgc
     5401 cgtggcgcgc ggccgggctt cattatcttg acgacgtgat caggcccagc gacacgcgcg
     5461 cggtgctgat ccgctcaatg gaactggcgc gcggaaacaa cggcgggcgc tcgcagcggc
     5521 ggctggcgaa ctggccgacg agtttctagg ctggcggcag cttcctccaa cccacccaga
     5581 accccagcgc cgccaccagc cccgcgagca ggaacacgct ctgcggcgac agcgcttccc
     5641 agagataacc cgccgacagc gagccgatgc tgccgcccag tccgtaggcc acggcgttgt
     5701 acaccgcctg cccgcgcccc tgcagccgcg ccgggaacag tcgcgtcaca tagtgcatgg
     5761 ccaccgcgtg gtaggcgccg aaggtgagcg cgtgcgacag ctgcgcgacg accagcactg
     5821 gccacgaatc cactgccagt gcagtgatgc tccagcgcag cgcggtggcg ccaagcgccg
     5881 cgagcatcag cgggcgcgcg ccaacctgcg ggatcagctt gc
//
LOCUS       DASZZC010000007        12850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1221152,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000007 DASZZC010000000
VERSION     DASZZC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 12850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12850
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221152"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..782)
                     /locus_tag="VHE37_00135"
     CDS             complement(18..782)
                     /locus_tag="VHE37_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT33959.1"
                     /translation="MMRFASLGSGSQGNATLIECGATRVLVDCGFMLRDCENRLRRLG
                     IAPDTLSAILVTHEHHDHIGGVSRVARKYSVPVWMTAGTRAGWLDPDVPQLRLCSPHE
                     DFVVGELTVRPFPVPHDAREPCHFVFSDGRHRVGVLSDAGAVTPHMRACLSGLDALML
                     EFNHDLQMLAAGPYPPVLKARVGGNLGHLSNRQSAQLLRDIDISRLQHLVLTHISQKN
                     NTPELAYAAASEALGSAPPWLACARQDEGFAWREVA"
     gene            complement(790..1131)
                     /locus_tag="VHE37_00140"
     CDS             complement(790..1131)
                     /locus_tag="VHE37_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HVT33960.1"
                     /translation="MASAKHRLYRISFHNQSQVYELYAREVSHGSMVGFVEIGKLVFG
                     EKSTVVVDTSEEKLKAEFENVERFYVPLHAVIRIDEVNRQGPVRILPGSGEAGAQPST
                     VSYPNFGGPRK"
     gene            complement(1138..2547)
                     /locus_tag="VHE37_00145"
     CDS             complement(1138..2547)
                     /locus_tag="VHE37_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33961.1"
                     /translation="MRSGALALACALLLCACGRDDARPDLRRLYDAGMRPQMQPPVIV
                     IPGILGSRLRLKATGASVWPGSPWSLLLGDKQQLALRIDARTLEPVDDGIEADGLFEQ
                     AFGRDFYGEILRTLERSGGYVRGKPGEKCDPAQRRYYVFAYDWRQDNVLTARKLDALI
                     EQIRRDYGDPQLKVDVVAHSMGGLVTRYFLRYGTADVLERNDFPVTLAGAAKLRTVIL
                     LGTPNLGSVESLHGFLAGANVGLSRIPTEVLATLPSMYQLFPHPLNNWLVTPQGQKLE
                     RDLFDVDTWKRFQWSVFDPQVEQHLRAAGADVATLQRWFGKRLERARRFVWSLTVPLP
                     RTPVKLVVFGGDCTPTPARLLVEEDNGDSVVRLWPDEVRTRLPGVDYARLMLEPGDGA
                     VTKPSLLARESLDPTAERSKYLFFPLAYSFFLCERHDRLTGNISFQDNLLNVLLSQER
                     PWDNRESMPPQMRAGRGVR"
     gene            complement(2540..3406)
                     /locus_tag="VHE37_00150"
     CDS             complement(2540..3406)
                     /locus_tag="VHE37_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVT33962.1"
                     /translation="MLNRSGEAALAAVLSAHQLTASITDDVTYCGQWQEREPATARAT
                     FHLLASGQCRVESGALADAVTLGAGDLVIFSRGAAHALCSVSSPTGGWQDTTMLCGEL
                     DFVTGTRNPVLEALPECFVVRGDDSEPQFAELARLLCRESHRRDFGSRVVLDKLADAL
                     FVMAVRHYLNRNQDQRGLLAALLDAKLAQALDAMHSRPGQDWTVLDLARVACMSRTAF
                     ALRFSEVLGASPHQYLTSLRMTEALRLLKDPRLSMAAISERLGYQSEAAFRRSFKRVH
                     GQGPGAVRRAHA"
     gene            3480..4097
                     /locus_tag="VHE37_00155"
     CDS             3480..4097
                     /locus_tag="VHE37_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT33963.1"
                     /translation="MKLLNFAVWAHSLLDRLRAADWLAPLAMRLYLVPIFWQAGINKF
                     ENFSDIVDWFGSMDMGLGLPLPYVMATLATGAELVGAASLLLGLGLRWMCIPMMVTMI
                     VAILTVHLPNGWLAVSEGMGPFATERTMHAIERLNEIKDLVREHGDYDRLTEYGPIVI
                     LNNGVEFAATYFIMLLSLFFTGAGRFVSVDYWLRRRFMPQAVRAN"
     gene            4337..5707
                     /locus_tag="VHE37_00160"
     CDS             4337..5707
                     /locus_tag="VHE37_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33964.1"
                     /translation="MKRSWLVAVLFAAALGGGCSGGIPGASGGAPGKSTQADSSLPGS
                     YHDPASRCVSPQPSKQTSIAVAKWTLSDQAVTSDHVSPYMAASFVQATAPYTFNQAPS
                     WANDGRVLGGMDGDKTQIYNAQLDGSDAHCLTCSHPGDTADGKPAKNGLPQMRPQGDW
                     IMFESTRGHSVVYSGDGLGGAGDAIWVMRPDGSCPVQLTGVGSDGEIADDFHAYWSPD
                     GKHINWTHLSGNVAVDNYDTDTEFTMRVADFIDDGTNPPRVDNIINVGERGEAYETEL
                     WAPDGSGFLFQYGDQGDNQEIYFIRLYGNGATPTAPLVQHLSDGTPGWDEQALFTPDM
                     SAVIYMSSKDCSTCSYNIQTALEQFLHVPPLPDRFDGLAYVGLFFEAVLPAFVLPPQD
                     GGFSTDLYLLDLHTKSLRRLTNDNGVIPEFYFDAQGRKLIWSENHVYGGAPKNNTQTA
                     YFEGLP"
     gene            5793..6440
                     /locus_tag="VHE37_00165"
     CDS             5793..6440
                     /locus_tag="VHE37_00165"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HVT33965.1"
                     /translation="MKPYLALLLPAALLSGCATLSESECRTANWRDLGRADGQHGYEA
                     TRLGEHQDACGKYGIAPDAAAYQAGRDEGLRLYCTPQVALGEGRAGNEYHAVCPGDAN
                     LMFVRYYQRGSVQHQLDAEAGEIQQELDAQNQAKDATGDLGLYKMLEQNGRYLERTLH
                     RVQGLQQRAQFDIDADRDPQVYASGRWRSDLPYPDARAQADKWRQQHQHDHDKDH"
     gene            complement(6465..7424)
                     /locus_tag="VHE37_00170"
     CDS             complement(6465..7424)
                     /locus_tag="VHE37_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVT33966.1"
                     /translation="MKRFGEQSRAVACAAAGLMMLACAGTAVAAGPAAKPAAAAQVVP
                     VALETAKPTQRVSDFGLKSGAALVVDLDSGETLALKNPDQVMPIASLTKLMTALVVVE
                     AAQPMDQVLEITNEDVDIWRHSSSRLRVGTRLTREQMLLLALMSSENRAAMALSDNYP
                     GGRDAFIMKMNQKAQALGMSSSHFADPAGLSNNSVSTARDLHKLMTAAYAQPLIRSYT
                     TRPEASVRMGRRMVSFINSNRMVRRSGDWNIELQKTGFTNEAGRCLVMEVRVLSRRLA
                     MVFLDSVGTLTRYGDALRVRHHLELEAKASRKGGVASAAAAAS"
     gene            complement(7547..8317)
                     /locus_tag="VHE37_00175"
     CDS             complement(7547..8317)
                     /locus_tag="VHE37_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33967.1"
                     /translation="MWRALRIALLFLLMLGVGADAWLTRARSTSWQNTLWVGIYPIAG
                     DDSADTARYLAALTRDDFLPIEDFMAREAHRHGIALGQPLHVVRGRTLAELPPAPPRD
                     RSPLSIAQWSLQLRWWSHEVESAQPGPPSNIRVYVIYHDPQRQPVVPHSLGLQKGLIG
                     VVHAFASSAQGGSNDVVIAHELMHTLGATDKYDPVSTLPSFPDGYADPQAQPRWPQAR
                     AEIMGGRVPLSATQAEIPRSLASVVVGPATAREIGWTR"
     gene            8316..8483
                     /locus_tag="VHE37_00180"
     CDS             8316..8483
                     /locus_tag="VHE37_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33968.1"
                     /translation="MRDQPRMDAQASAKPSTAPALGMLFFIDRLNIYTIFNNMITRGA
                     VEACAPFGSRQ"
     gene            complement(8477..>12850)
                     /locus_tag="VHE37_00185"
     CDS             complement(8477..>12850)
                     /locus_tag="VHE37_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33969.1"
                     /translation="SGSASGTDLPSNFDCQNFKDTDWREPQGLTITYDPTVASSPQEL
                     FGVRGPATNKAWMVSTGRPDVLISVLDSGIEWQNKGDIQGVVNKIFLNRGELPKPVCA
                     NGQTGNAGDTRFGGYDCNGDGIFNIQDYAADPRVSDKNGNGILDPEDLILIFSDGVDN
                     DGNGYVDDISGWDFFENDNDPRDEVVYGHGTGEAKDSTGEAGLEDGQCPNCMILPLRV
                     GDSFVADVNHFAEGVVYAVDMGVNVIQEAEGDVNHTSFGQVAIDYAYSHGVIVNASEA
                     DENASHHMWPAAYDKTMVVNSVHAANTLFDQEAATVKPYSWLYFNACTNFGVYTYVSI
                     PSNSCSSEATGRSAGISGLLQSAARNAVERGQMTNYIDDDGSVKPWPLSAEELRQLWR
                     LGADDIDFSSPCVAGQGPQAHCTHAPAGVTANALPDNYITTIPTAMRYQTAKGWDFFT
                     GYGRANNGRTLRFIGLQGVSGVPDQVEGPAALTAQDRIPPEAWIETPRWFRQYGYRSD
                     NTLLIADAGGAPDEMVITGHVAANRVTSAGGSFDYVLEWAPQVQGPDYLSSGGADSSD
                     SDSEDRTDGPWHPIAPPSGLSITGLHAGYSGVLGRIKISDIIAGQAASSDPFNQLTDP
                     TSPFGAEVQDIRLRLRVIAHPASSADTINNEAISMKIVDVYPAVETIMRDDLGIHGMP
                     SDGGASPSFHDIDGDGRDELIVASGDGLVHAYTDVAKGTELPGWPVHTLPLSTVGLHG
                     VVNGGDVSYPDIHASGNNAYTSGKVESDFYSPILIGTPAVVDLDDDGQVETIVSDNEG
                     HVYAWEPDGSMRPGFPVTVNYDWTTPIRCQPSTIPNCDANTTVASHQLKRDEFNRKDW
                     GIISGVTVGDIDPSYSGMEIVAGASDGHLYAWHADGTRVDGWPVMLRDPAKVASVDPV
                     THFITHTADSGVKYDVAILPNPALADLDGDGKLEVISGVDEEYVEAPNVAIDPAVTAA
                     SALLGSSGNNRVYALYGDGNKHSGTAPANQPDAVAYLPGWPVKPAMLLTELLPTVGSG
                     TNTQPVIFPASGGSEIAVSSCCGPVYIFKPDGTSAFGSANGKDIALADTVFGAGSTAV
                     DRPAIAGVGALAVGSLDGGVHLSVVGPGTGLRRALSIAAVDEHSVGAEDQLAMWDTRT
                     GQYEPNAPIQVNDLQFFIAPIVADVSGNGLASAIQSTAVGDTVVAGLLSTEFTATRYH
                     TGGWIVASAAVGNGPVYGSDGKPDAQLYMTTITREGYLRMYPTGVDSTTSAGCAALKE
                     WPEKGHDARRSGNYSTDGERPYPVTQVQGTVADSKLTLKFLASGNDRNCGTADHYELR
                     GVTGNVDHPDWLTATRVMAQPVSGAAGTQESMTTPAFASGDFTLMLRAYDAAGNGSAP
                     AVLHLSVASGGGTGSTTGSTTGSTTGSTTGSTTGGTTGTTTGSSTGGTFGGVGGSGGG
                     AMPLLSLLLLGGLGALRRRRH"
BASE COUNT         2051 a         4390 c         4333 g         2076 t
ORIGIN      
        1 gaacgaaaaa agcggcgtca cgctacctcg cgccaggcaa acccttcgtc ctgacgcgcg
       61 caggccagcc agggcggcgc gctgcccagc gcctcgctcg ccgcggcgta ggccagctcc
      121 ggcgtgttgt tcttctgcga gatgtgcgtg agcaccaggt gctgcaggcg cgagatgtcg
      181 atgtcgcgca gcagctgcgc cgactggcgg tttgaaagat gaccgagatt gccgccaacg
      241 cgcgccttca gcaccggcgg gtacggaccc gccgccagca tctgcagatc gtggttgaac
      301 tcgagcatca gcgcgtcgag ccccgacagg caggcgcgca tgtgcggcgt caccgcgcct
      361 gcgtccgaca gcacgccgac gcggtggcgt ccatcggaga aaacgaaatg acacggctcg
      421 cgtgcatcgt gcggcaccgg gaacggccgc acggtgagct cgccgacgac gaaatcttcg
      481 tgcggactgc acagccgcag ctgcggcacg tcggggtcga gccagccggc gcgcgtgccg
      541 gcagtcatcc acaccggcac cgagtacttg cgcgcaacgc gcgacacgcc gccgatgtgg
      601 tcgtggtgct cgtgcgtgac caggatggcc gacagcgtat ccggcgcaat gccgagccgg
      661 cgcaggcggt tttcgcaatc gcgcagcatg aagccgcagt cgaccagcac gcgcgtggcg
      721 ccgcattcga tcagcgtcgc gttgccctgc gaaccgctgc ccagcgaagc aaagcgcatc
      781 attgccgcat cacttgcgcg ggccgccgaa gttcggatag ctgacggtgg aaggctgggc
      841 gccggcctcg ccgctgcccg gcaggatgcg caccgggccc tgcctgttca cctcgtcgat
      901 gcggatcacc gcatgcagcg gcacgtagaa gcgctcgacg ttctcgaact ccgccttcag
      961 tttttcttcg ctggtgtcga ccaccacggt ggacttctcg ccgaacacca gcttgccgat
     1021 ctcgacaaag ccgaccatgg agccgtggct cacttcgcgc gcgtacagct cgtacacctg
     1081 cgactggttg tggaagctga tgcggtagag acggtgcttc gcggaggcca tgccgaatta
     1141 tcgcacgccc cgcccggcgc gcatctgcgg cggcatcgac tcgcggttgt cccacggccg
     1201 ctcctgcgac agcaacacgt taagcaggtt gtcctggaaa ctgatgttgc cagtcagccg
     1261 gtcgtggcgc tcgcacagga agaacgaata ggccagcgga aagaacaggt acttgctgcg
     1321 ctcggccgtg ggatcgaggc tttcccgcgc cagcagcgac ggcttggtta ccgcgccgtc
     1381 gcctggctcc agcatcagcc gcgcgtaatc cactcccggc aaccgcgtgc gcacttcgtc
     1441 cggccacagg cgcaccaccg aatcgccgtt gtcttcttca accagcagcc gcgccggcgt
     1501 tggcgtgcaa tcgccgccga acaccaccag cttcaccggc gtgcgcggca agggcacggt
     1561 gagcgaccag acgaagcgcc gcgcgcgctc taggcgcttg ccgaaccagc gctgcagcgt
     1621 ggccacgtcg gcaccggcgg cgcgcaggtg ctgttccacc tgcgggtcga acacgctcca
     1681 ctggaaacgc ttccaggtgt cgacgtcgaa caggtcgcgt tcgagtttct ggccctgcgg
     1741 cgtcaccagc cagttgttta gaggatgcgg aaacagctgg tacatgctgg gcagcgtggc
     1801 cagcacctcg gtcggaatgc gcgacaggcc gacgttggcg ccggccagga agccgtgcag
     1861 cgattcaacc gagccgaggt tgggcgtgcc gagcaggatc accgtgcgca gcttggcggc
     1921 gccggcgagc gtcacgggaa aatcgttgcg ctcgagcacg tcggcggtgc cgtagcgcag
     1981 aaaatagcgc gtcaccaggc cgcccatgct gtgcgccacc acgtcgacct tcaactgcgg
     2041 atcgccgtag tcgcggcgga tctgctcgat cagggcgtcg agcttgcgcg cggtgagcac
     2101 attgtcctgg cgccagtcat aggcgaagac gtagtagcgg cgctgcgcag ggtcgcattt
     2161 ctcgcccggc ttgccgcgca cgtagccgcc cgagcgctcc agcgtgcgca ggatctcacc
     2221 ataaaaatct cggccgaaag cctgctcgaa caggccatcg gcttcaatgc cgtcatccac
     2281 cggctccagt gtgcgcgcgt cgattctcag cgccagctgc tgcttgtcgc ccagcagcag
     2341 cgaccacggc gacccgggcc acacgcttgc gccggtggcc ttcaggcgca ggcgtgagcc
     2401 caggatgccg ggtatgacga ttaccggcgg ctgcatctgc ggccgcatgc ctgcatcgta
     2461 gagccggcgc aggtccggcc gcgcgtcgtc gcgcccgcag gcgcacagca gcagcgcgca
     2521 agccagcgcc agcgcgccgc tacgcatgcg cgcgcctcac cgcccccggc ccctgcccgt
     2581 gcacgcgctt gaagctgcgc cggaacgcgg cttccgactg gtaacccagg cgctcggaga
     2641 tcgccgccat cgacaggcgc gggtccttga gcaggcgcag cgcctcggtc atgcgcaggc
     2701 tggtgaggta ttggtgcggg ctggcgccga gcacttcgga gaaacgcagc gcgaacgcgg
     2761 tgcgcgacat gcaggccacg cgcgccagat ccagcacggt ccagtcctgc ccggggcgcg
     2821 agtgcatggc gtcgagcgcc tgcgccagct tggcgtcgag cagcgccgcc agcagcccgc
     2881 gctggtcttg gttgcggttg aggtagtggc gcaccgccat cacgaacagc gcgtcggcca
     2941 gcttgtccag caccacgcgg ctgccgaaat cgcgccggtg cgactcgcgg cacagcaggc
     3001 gcgccagctc ggcaaactgc ggctcgctgt cgtcgccgcg caccacgaag cattcgggca
     3061 gcgcttcgag caccgggttg cgcgtgccgg tgacgaagtc cagctcgccg cacagcatgg
     3121 tggtgtcctg ccagccgccg gttggcgagc tgacgctgca cagcgcgtgc gcggcgccgc
     3181 ggctgaagat gaccaggtcg ccggcgccga gcgtcaccgc atccgccagc gcgccggatt
     3241 ccacgcggca ttgcccggac gcgagcaggt gaaacgtcgc gcgcgcggtc gccggctcgc
     3301 gctcctgcca ctgcccgcag taggtcacgt cgtcggtgat cgacgcggtg agctggtgcg
     3361 ccgacagcac cgcggccagc gcggcttcgc ctgaacgatt gagcatatct cccgtacgcc
     3421 ggaaggtgat gtgcggatgc ggcgcaggct agcatgcgtc atccatcggg ggagccttca
     3481 tgaaactgct gaacttcgcg gtatgggcgc acagcctgct cgaccggctg cgcgccgccg
     3541 actggctggc gccgctggcc atgcgcctgt acctggtgcc gatcttctgg caggccggca
     3601 tcaacaagtt cgagaatttc tccgacatcg tcgactggtt cggcagcatg gacatgggcc
     3661 tgggcctgcc gctgccgtac gtgatggcga cgctggccac cggcgccgaa ctggtcggcg
     3721 ccgcctcgct gctgctcggc ttaggcctgc gctggatgtg cataccgatg atggtcacca
     3781 tgatcgtcgc catcctcacc gtgcacctgc ccaacggctg gctggcggtt tccgagggca
     3841 tggggccctt cgccaccgag cgcaccatgc acgccataga gcggctcaac gagatcaagg
     3901 acctggtgcg cgagcacggc gactacgacc ggctgaccga gtacggcccc atcgtcatcc
     3961 tcaacaacgg cgtcgagttc gccgccacct acttcatcat gctgctgtcg ctgttcttca
     4021 ccggtgccgg gcgtttcgtc agcgtcgact actggctgcg ccggcgcttc atgccgcagg
     4081 ccgtgcgcgc gaactgaccc gggccggcgc gagactcgca ggcctcgccc ggccgcaccc
     4141 attgccgcgt ggccatgcgg ctggctggcg ccgaccggtc ggacctggtc caggcgcatc
     4201 agccgaagta aaaaagcact accgggcgcc atcacgcggc tggaccaggc agactcgccg
     4261 ctggaatcgg cgcaagcggc cagcgaagcc gcgcaccgca ccggcacggc cgggagaaac
     4321 gcaggggaga caggcgatga agcgatcatg gctggtggcc gtgctgttcg cggcggcgct
     4381 gggcggcggc tgcagcggcg gcattcccgg cgcaagcggc ggcgcgccgg gcaagagcac
     4441 gcaggccgac agcagcctgc cgggcagcta tcacgacccg gcgtcgcgct gcgtcagccc
     4501 gcagccgtcg aagcagacca gcatcgccgt tgccaagtgg acgctgagcg accaggccgt
     4561 cacttccgac catgtcagcc cttacatggc ggccagcttt gtgcaggcca ccgcgcccta
     4621 caccttcaac caggcgccgt cctgggccaa cgacggccgc gtgcttggcg gcatggacgg
     4681 cgacaagacg cagatctaca acgcgcagct tgatggcagt gacgcccact gcctcacctg
     4741 cagccacccc ggcgacaccg ccgacggcaa gccggccaag aacggcctgc cgcagatgcg
     4801 cccgcagggc gactggatca tgttcgagtc cacgcgcggc cacagcgtgg tctattccgg
     4861 cgacggcctc ggcggcgccg gcgatgcgat ctgggtaatg cggcccgacg gctcctgccc
     4921 ggtgcagctg accggcgtgg gcagcgacgg cgagatcgcc gacgatttcc acgcctactg
     4981 gtctcccgac ggaaagcaca tcaactggac gcacctgtcg ggcaacgtcg cggtggacaa
     5041 ctacgacacc gataccgagt tcaccatgcg cgttgccgac ttcatcgacg acggcacgaa
     5101 tccgccgcgc gtggacaaca tcatcaacgt cggcgaacgc ggcgaggcct acgagaccga
     5161 gctgtgggcg cccgacggca gcggcttcct gttccagtac ggcgaccagg gcgacaacca
     5221 ggagatctac ttcatccgcc tgtacggcaa cggcgccacg ccgaccgcgc cgctggtgca
     5281 gcacctgtcc gacggcacgc cgggctggga cgagcaggcg ctgttcacgc ccgacatgag
     5341 cgcggtgatc tacatgtcga gcaaggactg cagcacctgc agctacaaca tccagaccgc
     5401 gctggagcag ttcctgcacg tgccgccgct gccggaccgc ttcgacggcc tggcttacgt
     5461 cggcctgttc ttcgaggcgg tgctgcccgc cttcgtgctg ccgccgcagg atggcggctt
     5521 ctccaccgat ctctacctgc tcgacctgca caccaagtcc ttgcggcgtc tcaccaacga
     5581 caatggcgtg atacccgagt tctatttcga cgcgcagggc aggaagctga tctggtcgga
     5641 aaaccacgtc tacggcggcg caccgaagaa caacacgcag accgcgtatt tcgaaggcct
     5701 gccataacgt cggcattgcc aaaatcccgc ggccctgcga agttgccggc acaacaaggc
     5761 ggcgcagccg tcgcatcaac gggagtcaat cgatgaagcc gtaccttgcc ctgctgctgc
     5821 ccgcggcgct gctgtccggt tgcgccacgc tcagcgaaag cgaatgccgc accgccaact
     5881 ggcgcgacct gggccgcgcc gacggccagc acggctacga ggccacgcgc ctgggcgaac
     5941 accaggatgc gtgcggcaag tacggcattg cgcccgatgc cgccgcttac caggccgggc
     6001 gcgacgaagg cctgcgcctg tactgcacgc cgcaggtagc gctgggcgaa ggccgcgccg
     6061 gcaacgagta ccacgcggtg tgcccgggcg acgccaacct gatgttcgtg cgctactacc
     6121 agcgcggcag cgtgcagcac cagctggacg ccgaagccgg cgagatccag caggaactcg
     6181 acgcgcagaa ccaggccaag gacgccaccg gcgacctggg cctgtacaag atgctggagc
     6241 agaacggccg ctacctggag cgcacgctgc atcgcgtcca gggcctgcag cagcgcgcgc
     6301 agttcgatat cgacgccgac cgcgatccgc aggtctatgc ctcaggccgc tggcgcagcg
     6361 acctgccgta tcccgacgcg cgcgcgcagg ccgacaagtg gcgccagcag caccagcacg
     6421 atcacgacaa ggatcattga gcggcgcgcg ggcgccgcgt cagttcaaga ggcggcagcc
     6481 gcggcgctgg ccacgccgcc cttgcggctg gccttggctt cgagttcgag gtggtggcgc
     6541 acgcgcagtg catcgccata gcgcgtcagc gtgccgaccg aatccaggaa caccatggcc
     6601 aggcggcggc tgagcacgcg cacttccatc accaggcagc ggccggcctc gttggtgaag
     6661 ccggtcttct gcagctcgat gttccagtcg ccggaacggc gcaccatgcg gttgctgttg
     6721 atgaagctga ccatgcggcg gcccatgcgc acgctggctt ccggccgcgt ggtgtagctg
     6781 cggatcagcg gctgcgcgta ggcggcggtc atcagcttgt gcaggtcgcg cgcggtggac
     6841 acgctgttgt tcgacaggcc ggcgggatcg gcgaaatggc tcgagctcat gcccagcgcc
     6901 tgcgccttct ggttcatctt catgatgaag gcgtcgcggc cgccggggta attgtccgac
     6961 agcgccatgg ccgcgcggtt ttccgacgac atcagcgcca gcagcagcat ctgctcgcgc
     7021 gtcaggcgcg tgcccacgcg caggcgcgag ctgctgtgcc gccagatgtc gacatcctcg
     7081 ttggtgatct ccagcacctg gtccatcggc tgcgccgctt ccaccaccac cagcgcggtc
     7141 atcagcttgg tcagcgaggc gatcggcatc acctggtcgg gattcttcag cgccagtgtc
     7201 tcgccgctgt ccaggtccac caccagcgcg gcgccagact tgagaccgaa atcgctcacc
     7261 cgctgcgtgg gcttggcggt ttccagcgcc accggcacca cttgcgcggc ggcagccggc
     7321 ttcgcggccg ggccggcggc aacagccgtt ccggcgcagg ccagcatcat cagtccggcg
     7381 gcagcgcatg caacggcgcg gctctgctcc ccaaatcgct tcacgttctt ccccacctgc
     7441 ttgaaacttg tccgcatact ttatccgata atttgtcaaa ttctagcggc aagccgtccg
     7501 cggcagaaga cgacaatttg gcctacgtcg agctctgcag ttccgttcac cgcgtccagc
     7561 cgatctcgcg cgccgtcgcc ggccccacca ccacgctggc caggctgcgc ggaatctccg
     7621 cctgcgtggc cgacagcggc acgcggccgc ccatgatttc ggcgcgcgcc tgcggccagc
     7681 gcggctgggc ctgcggatcg gcgtaaccat cgggaaagga cggcagcgtg ctgaccggat
     7741 cgtacttgtc ggtcgcgccc agcgtgtgca tcagctcgtg ggcgatgacc acgtcgttcg
     7801 atccgccctg cgcgctgctg gcgaacgcat gcaccacgcc gatcagccct ttctgcaaac
     7861 ccagtgaatg cggcaccacc ggctggcgct gcgggtcgtg gtagatgacg tagacgcgga
     7921 tgttggacgg cggacccggc tgcgcgcttt ccacctcgtg cgaccaccag cgcagctgca
     7981 ggctccattg cgcgatgctc aagggcgaac gatcgcgcgg cggtgccggt ggcagttccg
     8041 caagcgtgcg ccctcgcacc acgtgcagcg gctggccaag cgcgatgccg tggcgatgcg
     8101 cctcgcgcgc catgaagtcc tcgatcggca ggaagtcgtc gcgcgtcagc gcggccaggt
     8161 agcgcgcggt gtcggcgctg tcgtcgccgg cgatcgggta gatgcccacc cacagcgtgt
     8221 tctgccagct ggtgctgcgc gcgcgcgtga gccaggcgtc ggcgcccacg ccaagcatga
     8281 gcaggaacaa cagcgcgatg cgcagcgcgc gccacatgcg cgatcagccg cgcatggacg
     8341 cgcaggcgtc cgcgaaacca tccaccgcac ccgccctcgg catgttattt tttattgatc
     8401 gattaaacat ttatacaatc tttaataaca tgattacccg cggtgcggtg gaggcttgcg
     8461 cgcccttcgg cagccgtcaa tgacgccggc ggcgcaacgc gccgaggccg ccgagcagca
     8521 gcagggacag cagcggcatt gcaccaccac ccgaaccgcc gacgccgccg aacgtgccgc
     8581 cggtgctgct gcccgtggtc gtgccggtgg tgccgccggt ggtcgatcct gtcgtcgagc
     8641 cggtagtcga tccggtcgtc gagcccgtag tggaacccgt gccgccgccg gaggcaacgc
     8701 tcaggtgcag cactgccggc gccgagccgt tgcccgccgc gtcgtacgca cgcagcatca
     8761 gtgtgaaatc accggacgcg aaggccggcg tggtcatgct ctcctgtgtg ccggcggcgc
     8821 cgcttaccgg ctgcgccatc acgcgcgtgg cggtgagcca gtccggatga tcgacattgc
     8881 cggtgacgcc gcgcagctcg tagtgatccg ccgtgccgca gttgcggtca ttgccgctgg
     8941 ccaggaattt cagcgtgagc ttgctgtccg ccaccgtgcc ctgcacctgc gtcaccgggt
     9001 atggccgctc gccgtcagtg gagtagttgc cgctgcggcg cgcatcgtgg cccttctccg
     9061 gccattcctt cagcgcggca cagccggcgc tggtggtgga atcgacaccg gtgggataca
     9121 tgcgcaggta gccttcgcgc gtgatggtgg tcatgtacag ctgcgcatcg ggcttgccgt
     9181 cgctgccgta caccgggccg ttgccgaccg cggccgaagc cacgatccag ccgccggtgt
     9241 gatagcgcgt ggcggtgaac tcggtcgaca gcaacccggc gaccacggtg tcgcccacgg
     9301 cggtgctctg gatggccgag gccagcccgt tgccggaaac atcggccacg atcggcgcga
     9361 tgaagaactg caggtcgttc acctggatcg gcgcattggg ctcgtactgg ccagtgcgcg
     9421 tgtcccacat ggccagctgg tcttccgcgc cgaccgaatg ctcgtcgacc gcggcgatcg
     9481 acagcgcgcg gcgcagcccg gtgcccgggc cgaccaccga caggtgcaca ccgccatcga
     9541 gcgaaccgac cgccagcgcg ccgacgccgg cgatggccgg gcggtcgacg gcggtggagc
     9601 ccgcgccgaa cacggtgtct gcgagcgcga tgtccttgcc gttggcggag ccaaacgcgg
     9661 acgtgccgtc gggcttgaag atgtacaccg gcccgcagca cgacgacacc gcgatctcgc
     9721 tgccgcccga ggccgggaag atcaccggct gcgtgttggt gcccgacccc accgtgggca
     9781 gcagttcggt gagcagcatg gccggcttca ccggccagcc cggcaggtac gcaactgcat
     9841 ccggctgatt cgccggcgcg gtgccgctgt gcttgttgcc gtcgccgtac agcgcgtata
     9901 cgcggttgtt gccggaggaa ccgagcagcg cgctggccgc ggtcaccgcc ggatcgatgg
     9961 ccacgttcgg cgcttccacg tattcctcgt cgacaccgga gatcacctcg agcttgccgt
    10021 cgccgtcgag atcggccagc gccgggttgg gcaggatggc cacgtcgtac ttcacgccgg
    10081 aatcggcggt gtgggtgatg aaatgcgtga ccgggtcgac cgacgccacc ttggccggat
    10141 cgcgcagcat caccggccag ccgtcgacgc gcgtgccgtc ggcgtgccag gcgtaaagat
    10201 gcccgtcact cgcaccggcc acgatctcca tgccggaata cgacggatcg atgtcgccga
    10261 cggtgacgcc ggagatgatg ccccagtcct tgcggttgaa ctcgtcgcgc ttgagctggt
    10321 gcgacgcgac cgtggtgttg gcgtcgcagt tcgggatggt gctgggctgg cagcggatcg
    10381 gcgtggtcca gtcgtagttc accgtcaccg ggaagccggg ccgcatcgag ccatcgggct
    10441 cccatgcata cacgtggcct tcgttgtcgc tgacgatggt ttccacctgg ccgtcgtcat
    10501 cgagatcgac cacggctggc gtgccgatca ggatcggcga atagaagtcg ctctccacct
    10561 tgccactggt gtaggcgttg ttgccgctgg cgtggatgtc ggggtacgac acgtcgccgc
    10621 cgttgaccac gccgtgcagg ccgacggtgg acagcggcag cgtgtgcacc ggccagcccg
    10681 gcagctcggt gcccttggcc acatcggtgt acgcatgcac caggccgtcg cccgaagcga
    10741 cgatcagctc gtcgcggcca tcgccgtcga tgtcgtggaa cgagggcgaa gcgccgccgt
    10801 cgctgggcat gccgtggatg cccaggtcgt cgcgcatgat ggtttccacc gccggataca
    10861 cgtcgacgat cttcatgctg atcgcctcgt tgttgatggt gtcagcgctg cttgcgggat
    10921 gcgcgatcac gcgcaggcgc aggcggatat cctgcacttc cgcgccgaac gggctggtcg
    10981 gatcggtcag ctggttgaac ggatcggacg acgccgcctg gccggcgatg atgtcgctga
    11041 tcttgatccg gccgagcacg ccgctgtagc cggcatgcag gccggtgatg ctgaggccgc
    11101 tgggcggcgc gatgggatgc cacgggccgt cggtgcggtc ttcgctgtcg ctgtcgctgc
    11161 tgtcggcgcc gccggatgaa agatagtccg ggccctgtac ctgcggcgcc cactccagca
    11221 cgtaatcgaa gctgccgccg gcgctggtca cgcggttggc ggcgacatgg ccggtgatca
    11281 ccatctcgtc cggcgcgccg ccggcgtcgg cgatcagcag cgtgttgtcg ctgcggtagc
    11341 cgtattggcg gaaccagcgc ggcgtttcga tccaggcttc cggcggaatg cggtcctgcg
    11401 cggtaagggc ggccgggcct tcgacctggt cgggaactcc gctcacgccc tgcaggccga
    11461 tgaagcgcag cgtgcggccg ttgttggcgc ggccgtagcc ggtgaagaaa tcccagccct
    11521 tggccgtctg gtaacgcatg gcggtgggaa tggtggtgat gtagttgtcc ggcagcgcgt
    11581 tcgcggtcac gccagcgggc gcgtgcgtgc aatgcgcctg cgggccctgg ccggcgacgc
    11641 acggcgagga gaaatcgatg tcgtcggcgc ccaggcgcca cagctgacgc agttcctcgg
    11701 ccgacagcgg ccacggtttc accgagccgt cgtcgtcgat gtagttggtc atctgcccgc
    11761 gctcgaccgc gttgcgcgcc gccgactgca gcaggccgga gatgccggcg ctgcgcccgg
    11821 tggcttccga cgaacatgag ttcgacggga tcgacacgta ggtgtacacg ccgaagttgg
    11881 tgcaggcgtt gaagtacagc cagctgtacg gcttgaccgt cgccgcttcc tggtcgaaca
    11941 gcgtgttggc cgcgtgcacc gagttgacca ccatggtctt gtcgtacgcc gccggccaca
    12001 tgtgatggct ggcgttctcg tcggcttccg acgcattgac gatgacgccg tggctgtagg
    12061 cgtagtcgat cgccacctgg ccgaagctgg tgtggttgac gtcgccttcc gcttcctgga
    12121 tgacgttgac gcccatgtcg accgcgtaca ccacgccctc ggcgaagtga ttgacgtcgg
    12181 ccacgaagga gtcgcccacg cgcagcggca ggatcatgca gttggggcat tggccgtctt
    12241 ccagcccggc ctcgccggtg gagtccttgg cctcgccagt gccgtggccg tacaccactt
    12301 catcgcgcgg gtcgttgtcg ttctcgaaga aatcccagcc ggaaatgtcg tccacgtaac
    12361 cgttgccgtc gttgtcgacg ccgtcggaga aaatcaggat gaggtcttcg ggatcgagga
    12421 tgccgttgcc gttcttgtcc gatacgcgcg ggtcggcggc gtagtcctgg atgttgaaga
    12481 tgccgtcgcc gttgcagtcg tagccgccga agcgggtgtc gcccgcattg ccggtctggc
    12541 cgttggcgca caccggcttg ggtagctcgc cgcggttgag gaagatcttg ttgaccacgc
    12601 cctggatgtc gcccttgttc tgccactcga tgccggagtc gagcaccgag atcagcacgt
    12661 cgggccggcc ggtggacacc atccacgcct tgttggtcgc cggtccgcgc acaccgaaca
    12721 gttcctgcgg gctgctcgcc accgtcgggt cgtaggtgat ggtcaggccc tgcggctcgc
    12781 gccagtcggt gtccttgaag ttctggcagt cgaagttcga cggcaggtcg gtgccggacg
    12841 cgctgccgct
//
LOCUS       DASZZC010000008         5292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_771232,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000008 DASZZC010000000
VERSION     DASZZC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771232"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /gene="bcsG"
                     /locus_tag="VHE37_00190"
     CDS             <1..1437
                     /gene="bcsG"
                     /locus_tag="VHE37_00190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HVT33970.1"
                     /translation="QALALPLGFALFWSDSFLPPFKHMIAQAGQLATFRASYLLELAG
                     RLVNFWMLGALVVLLALHLLLARKLRMASFALLALAAACAEPLWQHPAALPQAAGDAT
                     LSVAGGQAADNAAPTAAALDAALNRFYADESKRRVAFPAAMERKTPFDVVYLQICSLA
                     QDDLDYAGTAGHPLWNRFNLRFSNFSTGASYSGPAAIRLLRSGCGQPKHEDLYKPTDG
                     ACYLFDDLKRAGFEPQWLLNHSGVFGNFIGTIQTNGGMSMPPLKFPDAAVAMRSFDDQ
                     PVQDDYDVLAHWWAQRLKDKTPQVSLFYNTVTLHDGNRLPGAPRMDPRESFAVRSKKM
                     FDDFGRFFDLVQASGRRMVVVLVPEHGANVRGDKMQISGLREIPSPAIALAPVGIGLI
                     NLQPSAPLPPQLVVSDPTAHLAISHVVSQLLINSPFDNPPQDWKPYVTDLPQTNFVAE
                     NEGMIVMRVGTRYYLRNPDTSWVEYSTQ"
     gene            complement(1467..1847)
                     /locus_tag="VHE37_00195"
     CDS             complement(1467..1847)
                     /locus_tag="VHE37_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33971.1"
                     /translation="MAVARIRWRDEPKASDYPAAENYLLLIYPRKEAAALVRHLRGEP
                     ISRFKAIDVLRASRELLLDVTNSHVKEDHQKILNGQKLSPPLLVRHPRNARMLIVDGY
                     HRICAVCALDEDEIIPCKIVSARK"
     gene            2015..2842
                     /locus_tag="VHE37_00200"
     CDS             2015..2842
                     /locus_tag="VHE37_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33972.1"
                     /translation="MTSATWTDTFIRDYTVPLWLMKLGALLNFYFMLTTPSIFLAGVD
                     PWLVGSAQFVFAASAYRCLFPVHYEHDVVFHDSAFSSIFVTRTLATIKEIAFIYLMSL
                     VLRELNTAHIAWIDALSWLMVVLVMVAECFVWGAVLLYRFRYYVYEESTWLFIAIANT
                     IASIVLWQQGTEPMSGRGLLLGFNFAFAVSYLPFQLLNLRFLWLNADRNPPPAAGGRP
                     LSARLAEGMWRSIRTKNRRTDPESWGGWLGLLWMIGYFATFVPAWMYCIARVLTKTG"
     gene            complement(2921..4987)
                     /locus_tag="VHE37_00205"
     CDS             complement(2921..4987)
                     /locus_tag="VHE37_00205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVT33973.1"
                     /translation="MITRLDMRVVRAACAIALLFAGMAPARAATPANGTISPGSTQLS
                     FTGGPFYVPNQTDAGYIQAGVPDPICASPGTDCDLIPLTADLPDDYATTHPNDVIAIK
                     TSWTPVTGTGGATDACFATYLLDATGAVVNAAYTCANPEVMIQPAGEKQTQYTIEIIP
                     ELPLGQSYTTTVELTTAGASSGAALPPNGVPPLPVAFSQTISPEGMGDNAGEPSIGVD
                     WNPNNAALKHDLVNTGGVVFFQSGPNTLRVSFDDATVPATALWEDVSTPLVYQFVLSD
                     PIGFVDHMTGRVFALDLIGVEGQSFTAFSDDDGATWTPSEGGPPGGSPDHETLASGPY
                     AGGGPLPGLGPLYPSAVYYCGQAIVNNFCARSDDGGLLFGPQIPTLLPTDCAPAGAQN
                     GHVKVGPDGAVYLPQGKCPGLIGANVAGISVSQDNGLTWTYTAVPNSSSISLDPSVGI
                     DGGNNAYLTWLDNAAQPHVAVSTDHGATWSNDTLIGAPFGIQSANLVIAAAGDAGRAA
                     VGFIGTTTAGDPNGTNTFRGVWHLYIAMTYDAGKTWTTIDATPNDPVQVGSVCTSGTT
                     CGADRNLLDFNDLQVDREGRVLAAYAKGCLAPDCVTATALSAKPPYDASRANKAVVVR
                     QACGLSLFAAYDDKFKAQACPAVQGAQAAVTGATSSPGRFGGALPWALLLPLAGFAAL
                     RRRRMR"
BASE COUNT          862 a         1747 c         1764 g          919 t
ORIGIN      
        1 caggccctcg cgctgccgct gggctttgcg ctgttctggt ccgactcgtt cctgccgccg
       61 ttcaagcaca tgatcgcgca ggccgggcag ctggccacct tccgcgcgtc gtacctgctg
      121 gagctggccg gacggctggt caacttctgg atgctcggtg cgctggtcgt attgctggca
      181 ctgcacctgc tgctggcgcg caagctgcgc atggccagct tcgcgctgct ggcgctggcg
      241 gccgcctgcg ccgagccgct gtggcagcac ccggcggcgc tgccgcaggc ggccggcgac
      301 gctacgcttt ccgtagcagg cggccaggcg gcggacaacg ccgcgccgac cgcggccgcg
      361 ctcgatgccg cgctcaaccg cttctacgcc gacgagagca agcggcgcgt ggccttcccc
      421 gcggcgatgg agcgcaagac cccgttcgac gtggtctacc tgcagatctg ctcgctggcg
      481 caggacgatc tcgattacgc cggcaccgcc ggccacccgc tgtggaaccg cttcaacctg
      541 cgcttctcca atttctccac cggcgccagc tacagcgggc ctgccgccat ccgcctgctg
      601 cgctccggct gcggccagcc gaagcacgaa gacctgtaca agcccaccga cggcgcctgc
      661 tacctgttcg acgacctcaa gcgcgccggc ttcgagccgc agtggctgct caaccacagc
      721 ggcgtgttcg gcaatttcat cggcaccatc cagaccaacg gcggcatgag catgccgccg
      781 ctcaaattcc ccgacgccgc ggtggccatg cgcagcttcg acgaccagcc ggtgcaggac
      841 gactacgatg tactggcgca ctggtgggcg cagcggctca aggacaagac gccgcaggtc
      901 tcgctgttct acaacacggt gacgctgcac gatggcaacc gcctgcccgg cgcgccgcgc
      961 atggacccgc gcgaaagctt cgccgtgcgc tcgaagaaaa tgttcgatga cttcggccgc
     1021 ttcttcgacc tggtgcaggc ctccggccgg cgcatggtgg tggtgctggt acccgagcac
     1081 ggcgccaacg tgcgcggcga caagatgcag atttccgggc tgcgcgagat ccccagcccg
     1141 gccatcgcgc tggcgccggt cggcatcggg ctgatcaacc tgcagcccag cgcgccgctg
     1201 ccgccgcagc tggtggtgag tgaccccact gcgcacctgg ccatctcgca cgtggtctcg
     1261 cagctgctga tcaacagccc gttcgacaat ccgccgcagg actggaagcc ttacgtcacc
     1321 gacctgccgc agaccaactt cgtcgccgag aacgagggca tgatcgtgat gcgcgtgggc
     1381 acgcgctatt acctgcgcaa tcccgatacc agctgggtgg agtactcaac ccagtagcgc
     1441 tgcggccgac cagcattgcg cagccgtcac ttccgcgcgg agacgatctt gcacgggatg
     1501 atttcatcct cgtccaaagc gcacaccgcg cagatgcggt gatagccgtc gacgatcagc
     1561 atgcgggcat tgcgcggatg gcgcaccagc agcggcggcg acagcttctg gccgttcagg
     1621 atcttctggt gatcttcctt gacgtgcgaa ttggtcacgt cgagcagcag ctcgcgcgac
     1681 gcccgcagca catcgatcgc cttgaaccgg ctgatgggct cgccgcgcag atggcgcacc
     1741 agggcggccg cttccttgcg cggatagatc agcagcagat aattctccgc cgccgggtaa
     1801 tcgctggcct ttggctcatc gcgccagcgg attcttgcaa cggccatggt cggtccacgc
     1861 agcaggtcag gcagggttgg ctcatgaaag accgttgcgc tcgcggagaa gttgcgtagc
     1921 cggcggcaga tcaggcagcg ctgccatatt ccggcaggtc gtttctggta caaccgggac
     1981 tgtggcgcaa aaaaaatgga cccgggagga acccgtgaca agcgcgacct ggaccgacac
     2041 cttcatacgc gattacaccg tgccgctgtg gctgatgaag ctcggcgcgc tgctcaactt
     2101 ctatttcatg ctgacgacgc catcgatatt cctggccggt gtcgatccgt ggctggtcgg
     2161 ttctgcccag ttcgtctttg ccgcctcggc ctaccgttgc ctgttcccgg tgcactacga
     2221 gcacgacgtc gtgttccacg actcggcgtt ctcctcgatc ttcgtcaccc gcacgctggc
     2281 gacgatcaag gaaatcgcgt tcatctacct catgtcgctg gtgctgcgtg aactcaacac
     2341 cgcgcacatc gcctggatcg acgcgctgtc ctggctgatg gtggtgctgg tgatggtcgc
     2401 cgaatgcttc gtctggggcg cggtgctgct gtaccgcttc cgctactacg tctacgagga
     2461 atcgacctgg ctgttcatcg ccatcgccaa caccatcgcc agcatcgtgc tgtggcagca
     2521 gggcaccgag cccatgagcg gacgcgggct gctgctcggt ttcaacttcg ccttcgcggt
     2581 gagctacctc ccgttccagc tgctcaacct gcgtttcctg tggctcaacg cagaccgcaa
     2641 cccgcctccc gccgccggtg gacggccact gtcggcacgc ctcgccgagg gcatgtggcg
     2701 ctccatccgc accaagaacc gccgcaccga ccccgaatcc tggggcggct ggctggggct
     2761 gctgtggatg atcggctact tcgccacctt cgtcccggcg tggatgtatt gcatcgcgcg
     2821 ggtgctgacg aaaaccggct gaagccgcga gcgcgacgcg tggtcaaaaa aatgccggac
     2881 ccgcgtccgg catttttcgt ttcgcgacga agaccgggac tcagcgcatt cgacgccggc
     2941 gcaacgcggc gaagcccgcc agcggcagca gcagcgccca gggcagcgcg ccgccgaagc
     3001 ggccggggct gctggttgca ccggtgacgg cggcctgcgc gccctgcacg gccggacagg
     3061 cctgcgcctt gaacttgtca tcgtaggcgg caaacaggct caggccgcag gcctggcgca
     3121 ccaccacggc cttgttggcg cgcgaagcat cgtaaggcgg cttggcgctg agcgcggttg
     3181 ccgtcacgca atccggcgcc aggcagccct tggcataagc cgccagcacg cggccttccc
     3241 tgtccacctg cagatcattg aagtccagaa ggttgcgatc ggcaccgcag gtggtgccgc
     3301 tggtgcagac cgagcccacc tgcaccggat cgttcggcgt ggcgtcgatc gtcgtccagg
     3361 tcttgccggc gtcgtaagtc atcgcgatgt acaagtgcca gacaccgcgg aaggtattgg
     3421 tgccgttggg atcgccggcg gtggtggtgc cgatgaagcc caccgcggcg cggccggcat
     3481 cgccggccgc ggcgatgacc aagttggcgc tctgaatgcc gaacggcgcg ccgatcaggg
     3541 tgtcattgct ccaggtggcg ccatggtcgg tcgataccgc cacgtgcggc tgggcggcgt
     3601 tgtccagcca ggtgaggtag gcgttgttgc cgccatcgat gcccaccgac ggatcgaggc
     3661 tgatcgaact gctgttgggc accgcggtat acgtccaggt aagcccgttg tcctgcgaca
     3721 cgctgatgcc ggcaacgttg gcgccgatca ggccggggca tttgccctgc ggcaggtaga
     3781 cggcgccgtc gggcccgact ttcacgtggc cgttctgcgc gccggcgggt gcgcagtcgg
     3841 tcggcagcaa ggtcggaatc tgcgggccga acagcaggcc gccgtcgtcg ctgcgcgcgc
     3901 agaaattgtt gacgatggcc tggccgcagt aatacaccgc gctgggatag agcgggccga
     3961 ggcccggcag cgggccgccg ccggcgtagg gaccgctggc cagggtttcg tggtcgggcg
     4021 aaccgcccgg cgggccgcct tcggacggcg tccaggtcgc accgtcatca tcggagaagg
     4081 cggtgaagct ctggccttcc acgccgatca ggtcgagtgc gaacacgcga ccggtcatgt
     4141 ggtcgacgaa accgatcggg tcggacagca cgaactggta gaccagcggc gtggacacgt
     4201 cttcccacag cgccgtcgcc ggcacggtgg cgtcgtcgaa gctcacgcgc agcgtgttcg
     4261 ggccggactg gaaaaacacc acgccgccgg tgttcaccag atcatgcttg agcgcggcgt
     4321 tgttcggatt ccagtccacg ccaatggaag gctcgcccgc gttgtcgccc atgccttcgg
     4381 gcgaaatggt ctggctgaac gcaaccggca gcggcggcac gccgttgggc ggcagcgccg
     4441 cgccgctgct ggcgccggca gtggtgagct cgacggtggt ggtgtagctc tggcccagcg
     4501 gcagttcggg aatgatctcg atggtgtact gcgtctgctt ctcgccggcc ggctggatca
     4561 tcacctcggg gttggcgcag gtataggcgg cgttcaccac cgcgccggtg gcgtcgagca
     4621 ggtaggtggc gaagcaggcg tcggttgcgc cgccggtacc ggtgaccggc gtccagctgg
     4681 tcttgatggc gatgacgtcg ttgggatgcg tggtggcgta gtcatccggc aaatccgccg
     4741 tcagcggaat caggtcgcaa tcggtgccgg gactggcgca gatcggatcg gggacaccgg
     4801 cctggatata cccggcatcg gtctggttcg gcacgtagaa cgggccaccg gtaaaactca
     4861 gctgcgtgct gcccggagaa atggtgccgt tggccggcgt cgcagcgcgc gccggcgcca
     4921 tgccggcgaa cagcaacgcg atggcacatg ccgcgcgcac gacgcgcata tcaagcctgg
     4981 tgatcatggt gtttcctgtt cccgggtgcg gcgcaacgag gccgtcgttt gtaaatgaaa
     5041 agttaaattt cattcaatga aatgaatgac ttccgcaagg gtatcgactc cccgggctgg
     5101 cggaaatggt gagtttcact cagccgggat gccggctttt tgcgtggact gcagcgccgc
     5161 ccggcgcgga gatgcgaacc gggtgatacg ttccagcgct agaactgcat gtacgagcgc
     5221 accggcctgg gcggaaacac caccggcccc gcctgcaggt cgaagtcgta gcgcaggtag
     5281 gccgagaaga ag
//
LOCUS       DASZZC010000009         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1221293,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000009 DASZZC010000000
VERSION     DASZZC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221293"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VHE37_00210"
     CDS             complement(<1..418)
                     /locus_tag="VHE37_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33974.1"
                     /translation="MKKRLRVLLAFDMVYAVKPDHDYAEEFADRGNHETEIDVYQALL
                     ENGYEVRRLGLYDDPRRLLDAVREFQPDVIFNLVDTFHEITHFDKNMAALIELIGVPC
                     TGAASATLFLCNDKGLCRKILRFHRVRGPRFHTFYRG"
     gene            complement(425..1468)
                     /locus_tag="VHE37_00215"
     CDS             complement(425..1468)
                     /locus_tag="VHE37_00215"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HVT33975.1"
                     /translation="MSLDAAPAAAPLDLDTIGEEQLLDTRIRDLPLSIEGTWLEERVG
                     LLHAELQSKDLLFQPRCYLADEWLTPEGEPVIGIPFYLAHPRLIALEKKMMLEVEGES
                     PEHCMKLLRHEAGHALTYAFGLNRKRSWQRVFGHPSQEYDDAYRFRPYSKSFVRHLDS
                     YYAQNHPDEDFVETFAVWLTPGLDWRTRYRGWKALDKLEFVDRLMQSIAGQTPQKKSG
                     RQHWRASRIRSTLRHYYQKRRHAEAENYPDFHDANLRRMFPASGQPAENQRAVAPLLQ
                     AQRRALLGSIAEWTGESRFMVNEVLKAVLQRSRALQLLSSESEAVTLLRLSTYLTTLL
                     MNYRHTNRLRGKI"
     gene            1694..>2512
                     /locus_tag="VHE37_00220"
     CDS             1694..>2512
                     /locus_tag="VHE37_00220"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT33976.1"
                     /translation="MAQTAAGRRDPRVVIVGAGMSGMLMGIMLKRAGIESFEIYEKAA
                     KVGGTWRENSYPGLACDVPAYMYTYRFEPWAEWSRRFAHGPEIQKYFEHVYAKYGLEK
                     YIRCNAEVVSAVHEDGAWTVTLKDGTRTRADMVISAAGILHHPSLPDIPGLASFAGKS
                     FHSARWDHSVKLDDQRIGIIGTGSTAAQIVGALGGKVKKLVLFQRTAQWVHRIHDAEY
                     SEWSKRLMRRFTWLPKLMHWWYTTFFHVTFARIAVNSWWVMAALYWGCRSNLNSV"
BASE COUNT          445 a          783 c          838 g          446 t
ORIGIN      
        1 ggccgcggta gaaggtgtgg aagcgcggac cgcgcacgcg gtggaagcgc aggatcttgc
       61 ggcacaggcc cttgtcgttg cacaggaaca gcgtggccga cgccgcgccg gtgcagggca
      121 cgccgatcag ctcgatcagc gccgccatgt tcttgtcgaa atgcgtgatc tcgtggaagg
      181 tgtcgaccag gttgaagatc acgtcgggct ggaactcgcg caccgcgtcg agcagccggc
      241 gcggatcgtc gtacaggccc aggcgccgga cctcgtagcc gttctccagc agcgcctggt
      301 agacgtcgat ctccgtctcg tggttgccgc ggtcggcaaa ttcttccgcg taatcgtgat
      361 cgggcttgac cgcgtacacc atgtcgaatg ccagcagcac gcgcaggcgt tttttcatgt
      421 gcgcttaaat ctttccccgg agccggttgg tgtgacgata attcatcagc agggtggtga
      481 gataggtgga cagcctgagc agcgtcaccg cctcgctctc ggatgacagc agctgcagcg
      541 cgcggctgcg ctgcagcacc gccttgagca cctcgttgac catgaagcgg ctctcgccgg
      601 tccactcggc gatcgacccc agcagcgcgc gccgctgcgc ctgcagcagc ggcgccaccg
      661 cgcgctggtt ttccgcgggc tggccgctcg ccggaaacat gcggcgcagg ttggcatcgt
      721 ggaagtccgg gtagttctcc gcctcggcgt ggcggcgctt ctggtagtag tggcgcagcg
      781 tgctgcggat gcgcgaggcg cgccagtgct ggcggccact cttcttctgc ggcgtctggc
      841 cggcgatgga ctgcatcagg cggtcgacga actccagctt gtccagcgcc ttccagccac
      901 ggtagcgcgt gcgccagtcc agccccggcg tcagccacac ggcgaaggtc tcgacgaaat
      961 cctcgtcggg atggttctgc gcgtagtacg aatccaggtg ccgcacgaag ctcttgctgt
     1021 acgggcggaa gcggtaggcg tcgtcgtatt cctgcgacgg atggccgaac acgcgctgcc
     1081 agctgcgctt gcggttgagg ccgaaggcgt aggtcagcgc gtggcctgct tcgtggcgca
     1141 gcagcttcat gcagtgctcg ggcgattcgc cttccacttc gagcatcatc tttttttcca
     1201 gcgcgatcag ccgcggatgc gcgagataaa acgggatgcc gatcaccggc tcgccttccg
     1261 gcgtgagcca ctcgtcggcg agatagcagc gcggctggaa cagcaggtcc ttcgactgca
     1321 gttccgcgtg cagcagtccg acgcgctctt ccagccaggt gccttcgatg gaaaggggca
     1381 gatcgcggat gcgcgtgtcc agcaactgct cttcgccgat ggtgtcgagg tcgagcgggg
     1441 cggcggcagg ggcggcatcg aggctcatgg cgccaccata acaggcccgc ggcggcctca
     1501 aaaatgctgg accgcaccgc ccattacgat gttataaaag atctatcata tataaaaatg
     1561 taatataatt aataacacta aaacccggtg tgcggtggag gcatttttgg ccgcgcaagc
     1621 tgcccggacg agtaggtgac gcgccgcgcc ggagcgctag actgcgcgga cgaaatttcc
     1681 ggggaattgc gcaatggcac aaaccgctgc ggggcgccgc gatccgcgcg tggtgatcgt
     1741 cggcgccggc atgtccggca tgctgatggg catcatgctc aagcgcgccg gcatcgagag
     1801 cttcgagatc tacgagaagg ccgcgaaggt gggcggcacc tggcgcgaga acagctaccc
     1861 gggcctggcc tgcgacgtgc ccgcgtacat gtacacgtac cgcttcgagc catgggcgga
     1921 atggagccgg cgctttgcgc acgggccgga aatccagaag tacttcgagc atgtctatgc
     1981 caagtacggc ctggagaaat acatccgctg caacgcggaa gtggtaagcg cggtgcatga
     2041 ggacggcgcc tggacggtga cgctcaagga cggcacccgc acgcgcgccg acatggtcat
     2101 ctccgccgcc ggcatcctgc atcacccgag cctgcccgac atcccggggc tagccagctt
     2161 cgccggcaag agcttccaca gcgcacgctg ggatcactcg gtgaagctcg acgaccagcg
     2221 catcggcatc atcggcaccg gctccactgc ggcgcagatc gtcggcgcgc ttggcggcaa
     2281 ggtgaagaag ctggtgctgt tccagcgcac cgcgcagtgg gtgcaccgca tccatgacgc
     2341 cgagtactcc gagtggagca agaggttgat gcgccgcttc acctggctgc cgaagctgat
     2401 gcactggtgg tacaccacgt tcttccatgt gaccttcgcc cgcatcgcag tcaacagctg
     2461 gtgggtcatg gccgcgctct actggggctg ccggagcaat ctcaactcgg tg
//
LOCUS       DASZZC010000010         8725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_900075,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000010 DASZZC010000000
VERSION     DASZZC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900075"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1432
                     /gene="coaBC"
                     /locus_tag="VHE37_00225"
     CDS             176..1432
                     /gene="coaBC"
                     /locus_tag="VHE37_00225"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HVT33977.1"
                     /translation="MSTVTPLSLSGRRILLGVTGGIAAYKAADLCRRLVEVGATVQVV
                     MTRGAREFVTPLTFQALTGREVRDQLFDAANEAAMGHIELARWADAIVVAPASANFIA
                     RFAHGAADDLLATLCLASDRPIAIAPAMNRIMWSAAATQDNLALLRKRGTHVIGPGSG
                     GQACGEIGEGRMSEPLEIRDALVALLSSGPLAGLRAVVTAGPTREAIDPVRYISNRSS
                     GKMGYAVAQALASAGAQVTLVSGPTQLAVPGGVARVEVESAQQMLEATLAATPGADIF
                     VGAAAVADYRPETVAGEKIKKKDSALSLALTRTDDVLARVRAAHAQMFVVGFAAETEK
                     LEQHARAKLEAKKLDLIAANLVGRGRAFDRDDNELHLYWHGGGQELGRGDKSLLARAL
                     VEVIVQRIVATRGRTNAKVTSIKAKK"
     gene            1546..2001
                     /gene="dut"
                     /locus_tag="VHE37_00230"
     CDS             1546..2001
                     /gene="dut"
                     /locus_tag="VHE37_00230"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HVT33978.1"
                     /translation="MQKIELKILDPRVGKDIPLPAYATGGSAGLDLRAVLDAPLTLAP
                     GATTLLKTGIAIHIANPALAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSCWN
                     RGVEAFTINPGERIAQLVLVPVAHAQFEVVTEFHASERGAGGFGSTGKH"
     gene            2001..2867
                     /gene="argB"
                     /locus_tag="VHE37_00235"
     CDS             2001..2867
                     /gene="argB"
                     /locus_tag="VHE37_00235"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVT33979.1"
                     /translation="MAIDLTHAHNIAKVLTEALPYIRRFAGKTMVVKYGGNAMKDDAT
                     LAGFARDIVLMKAVGMNPVVVHGGGPQIGAHLEKLGKKSEFVDGMRVTDADTMDVVEM
                     MLGGLVNKSLVGAINQQGGRAVGLTGKDGGLVRARKLRGAGGKDLGQVGEVEKIDPRV
                     VAHLEAGGFIPVIAPVGVGEDGASYNINADLVAGKLASVLQAEKLMLLTNVQGLLDKN
                     KQLLTGLSPVDVEALIADGTIHGGMLPKIRCALDAVASGVKSAHIINGEIGHAVLLEL
                     FTDQGIGTLIRG"
     gene            complement(2872..3756)
                     /locus_tag="VHE37_00240"
     CDS             complement(2872..3756)
                     /locus_tag="VHE37_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33980.1"
                     /translation="MRNKTVVLGLLGCVLSVVALHVAAKGKQLVCWTDKDGVRACGDS
                     VPPEYAKQDRDVLNAQGQVVGTMAREKTAAELAEEQRQAEAAAAEQRRHTRQLAYDNY
                     MLQSYESVTQLQKVRDDNLTTIDGRLALAEKAVPGAENALNGVKARNAAEDAKDAKDH
                     VAYASAEDCIDPQIIKDKIAARAAKLPPPKPVKGKAPPPPKDPPELKAAQDKWNSREC
                     GQVRRGKLLAQFEQSLVDALKAVQSLKKQREDTAARYELDIARFKKLRSHEVMVGSVD
                     PNAPQGGAPAVASSAATP"
     gene            complement(3783..4424)
                     /gene="pyrE"
                     /locus_tag="VHE37_00245"
     CDS             complement(3783..4424)
                     /gene="pyrE"
                     /locus_tag="VHE37_00245"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HVT33981.1"
                     /translation="MQSYQSAFLSLALSSEVLRFGQFTLKSGRQSPYFFNLGKISSGA
                     AMRELGRAYAQALAASGVEYDMLFGPAYKGIPLVTTVAVALAEAGRDVPYAYNRKEAK
                     DHGEGGTLVGAPPKGRVVIVDDVLTAGTALREAVALLRSAGAQPVAAIIALDRQEKGA
                     SGRSAVQEMEQDSGVRVIPLVTLRDVLAYLGQGHVDAQTLAAMRAYQAQHCVA"
     gene            4474..5250
                     /locus_tag="VHE37_00250"
     CDS             4474..5250
                     /locus_tag="VHE37_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVT33982.1"
                     /translation="MRIITLNVNGIRSAARKGLFDWLGKQKADFLCLQEIKCHASDIE
                     GDELYSPAGWHAHFNHALKKGYAGTALFAREKPQEILDRLGDQEFDDHGRYVELRYGN
                     LSVVSLYVPSGSSGPEAQARKDRFLEFFLPKLREWKASGRDYVICGDWNIAHKNIDLK
                     NWRGNQKNSGFLPHERAWLDRVFGEAGFADAYRTLHPGLEGESYTWWSNRGAAWDKNV
                     GWRIDYHIVTPAMAKAVKRAKIYKDERFSDHAPLILDYAA"
     gene            5247..6602
                     /locus_tag="VHE37_00255"
     CDS             5247..6602
                     /locus_tag="VHE37_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT33983.1"
                     /translation="MSGTAASRFDALHVYAHPRVRGMLFLGFSAGLPFLLVFSTLSAW
                     LTQAGIARATIGMLSWIGISYSIKFFWSPVVDRARLPLLTNWLGRRRSWMLLGQCGIA
                     AGLCGIALHDPAADLEAVVWCALLVAFSSSTQDIAIDAWRIEAAPASMQGAMAAAYQF
                     GYRIAMLAASAGALWLAGEYGWHLSYAVMAVLVAVGLCTTLLVGEPQAQVSRDTLMRE
                     ARVLEFLETRAHWPEQLRRAGGWIIGAVVCPFVDFFARNGAARGALILALVGTFYLPY
                     ITMGVMTNPFYLDLGFSLQQIAAVAKGYGIVASLLGAFLGGIGVVRLGALRCMAIGAL
                     LVMFANLTYSALALLHQPTLAGLALIVSVDNIAYNFAAAAFVAYLSGLTNVSYTATQY
                     ALFSSLFNLPGKIIAGGSGALVDALGYAPFFIYTALLGIPALLVIAAITRKPTPPAAA
                     A"
     gene            complement(6565..7194)
                     /locus_tag="VHE37_00260"
     CDS             complement(6565..7194)
                     /locus_tag="VHE37_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT33984.1"
                     /translation="MLSWFATPQLHPAFRYALDDGTPILVRPLKPDDAPRLREGFRQL
                     SQLARRRRGLDINELSDEQLRELTTLDMRDRAAFGAANLEKPDEPGIGIARYHRVNGE
                     PRAADVQITIADDYQGRGAGFVLQACLHLTAHASGIRTLYYDVASDNDRILKHLKLLG
                     ARHAGRADNIDRLSMPVYCRAWDVPRGNDIGRRFAETFRRLRRAESVSA"
     gene            complement(7216..7524)
                     /locus_tag="VHE37_00265"
     CDS             complement(7216..7524)
                     /locus_tag="VHE37_00265"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HVT33985.1"
                     /translation="MAQWLRDMSVGEWYDVAGEPFEIVGIDAKAEIVLVQHFDGTLEE
                     FDFDTWVELAARPIPPPEDYSGALDLDKNDYGFDPEESIARGNGNPLDFLDRPGEELY
                     "
     gene            complement(7659..>8725)
                     /locus_tag="VHE37_00270"
     CDS             complement(7659..>8725)
                     /locus_tag="VHE37_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HVT33986.1"
                     /translation="TADRAPGASRMKYRRLIDGLPELGVVGLGTMTWGEQNSEAEAHA
                     QMDYALAHGVNLFDAAEMYPVPPRRETQGRTEQYIGSWLQKSGARKRIVLATKITGPG
                     DFPYLRGGTHLDRKSVLAACEGSLRRLQTDVIDLYQVHWPERNTNYFGKLGYAAREEA
                     HATPVEETLAALAELVRAGKVRHVGVSNETPWGVGEYLRLAREKNLPRIASIQNPYSL
                     LNRSFEVGLAEFAHREHLPLLAYSPLAMGVLAGKYLHGARPAGARMSLFTRFTRYLNE
                     EAELATREYVALAQRHGLDPAQMALAFVHSRPFVACTLIGATSMEQLKLNIASIDVGL
                     NDELLKAIEAIHRRHTIPCP"
BASE COUNT         1375 a         2934 c         2906 g         1510 t
ORIGIN      
        1 cgcgaaggcg ttcttgtagc cgttgcgctg cgtgaccagc gagttgtaga tgccgatgac
       61 gaaaaacacc agcaccacga gcactaccag gatgatccag cccatgcgcg tctccgagtg
      121 ttgagggtcg cgcggaactt agcatctatg gtcgcgggcg aatagaatcc cgcgcatgtc
      181 caccgtcacg ccattgtcac tgtccggccg ccgcatcctg ctcggcgtca ccggcggcat
      241 cgcggcctac aaggccgccg acctgtgccg ccgcctggtc gaggtgggcg ccaccgtgca
      301 ggtggtgatg acgcgcggcg cgcgcgagtt cgtcacgccg ctgaccttcc aggcgctcac
      361 cggccgcgaa gtgcgcgacc agttgttcga cgccgccaac gaagcggcca tgggccacat
      421 cgagctggcg cgctgggccg acgccattgt cgtcgcgcct gcctcggcca atttcatcgc
      481 gcggttcgca catggcgccg ccgacgacct gctggccacg ctgtgcctgg ccagcgaccg
      541 gccgatcgcc atcgcgccgg cgatgaaccg catcatgtgg agcgccgcgg ccacgcagga
      601 caacctcgcg ctgctgagaa aacgtggaac gcacgtaatc ggtcccggca gcggcggcca
      661 ggcctgcgga gaaatcggcg aaggccgcat gagcgagccg ctggagatcc gcgacgcgct
      721 cgtcgcattg ctgtcatccg gcccgctggc tggattgcgc gcggtggtga ccgcgggacc
      781 gacgcgcgag gcgatcgacc cggtgcgcta catcagcaat cgctcgtccg ggaaaatggg
      841 ttacgcggtg gcccaggcac tcgccagcgc cggcgcgcag gtcacgctgg tatccgggcc
      901 gacgcagctg gccgtgccgg gcggcgtggc gcgcgtcgag gtcgaatcgg cgcagcagat
      961 gctggaagcc acgctggcgg ccacgccggg cgcggacatt ttcgttggcg ccgccgcggt
     1021 ggccgactac cggcccgaga cggtcgctgg cgagaagatc aagaagaagg acagcgcgct
     1081 cagcctcgcg ctgacgcgca ccgacgatgt gctggcgcgc gtgcgcgccg cgcatgcgca
     1141 aatgttcgtc gtcggctttg ccgccgagac cgagaagctg gagcagcacg cgcgcgccaa
     1201 gctcgaggcc aagaagctgg acctgatcgc cgccaatctc gtaggccgcg ggcgcgcctt
     1261 cgaccgcgac gacaacgaac tgcacctgta ctggcacggc ggcggacagg aactcggccg
     1321 cggcgacaaa tcgcttttgg cgcgcgcgct ggtcgaggtc atcgtgcaac gcatcgtggc
     1381 gacgcgcggc aggaccaacg cgaaagtcac gtccatcaag gccaaaaaat gagtcaccgc
     1441 accgcccctg gtgatgttat taattattgt atatttttta attcattata ttttttataa
     1501 cacgccaaac cggggtgcgg tgaggcaaaa atcagcgcac cgaacatgca gaaaatcgaa
     1561 ctgaagatcc tcgacccgcg cgtgggcaaa gacattcccc tgcccgcgta tgccaccggc
     1621 ggctcggccg ggctcgacct gcgcgcggtg ctcgacgcgc cgctgacgct ggcgccgggt
     1681 gcgaccacgc tgctgaaaac cggcatcgcc atccacatcg ccaacccggc gctggccgcg
     1741 gtgattctgc cgcgctcggg cctcgggcat aagcacggca tcgtgctggg caacctggtg
     1801 ggcctgatcg attccgacta ccagggcgag ctgatggtga gctgctggaa ccgcggcgtt
     1861 gaggccttca ccatcaaccc gggcgagcgc atcgcccaac tggtgctggt gccggtggcg
     1921 cacgcgcagt tcgaggtggt gacggaattc cacgccagcg aacgcggcgc cggcggcttc
     1981 ggttccaccg ggaaacactg atggccatcg atctcaccca cgcccacaac atcgccaagg
     2041 tcctgaccga ggcgctgccc tacatccgcc gcttcgccgg caagaccatg gtggtcaagt
     2101 acggcggcaa cgcgatgaag gacgacgcca cgctggccgg cttcgcccgc gacatcgtgc
     2161 tgatgaaggc ggtcggcatg aatcccgtcg tggtgcacgg cggcggaccg cagatcggcg
     2221 cgcacctgga aaagctcggc aagaagagcg agttcgtcga cggcatgcgg gtgaccgacg
     2281 ccgacaccat ggacgtggtc gagatgatgc tcggcggcct ggtcaacaag tcgctggtcg
     2341 gcgccatcaa ccagcagggc ggccgcgcgg tgggactcac cggcaaggac ggcgggctgg
     2401 tgcgcgcgcg caagctgcgc ggcgccggcg gcaaggacct gggccaggtc ggcgaagtgg
     2461 agaagatcga tccgcgcgtg gtcgcgcacc tggaagccgg cggcttcatc ccggtgatcg
     2521 cgccggtggg cgtgggcgag gacggcgcgt cctacaacat caatgcggat ctcgtcgccg
     2581 gcaagctggc cagcgtgctg caggccgaga agctgatgct gctgaccaac gtgcagggcc
     2641 tgctcgacaa gaacaagcag ttgctcaccg gcctgtcacc ggttgatgtc gaagcgctga
     2701 tcgccgacgg caccatccat ggcggcatgc tgccgaagat ccgctgcgcg ctcgacgctg
     2761 tggccagcgg cgtgaagtcc gcgcacatca tcaacggcga gatcggccac gcggtgctgc
     2821 tggagctgtt taccgatcag ggcatcggca cgctgatccg cggatagcgc gctacggcgt
     2881 cgccgcgctg ctggcgacgg ccggcgcgcc gccctgcggc gcattgggat cgaccgagcc
     2941 gaccatgacc tcgtgcgagc gcagcttctt gaagcgcgcg atgtcgagtt cataacgcgc
     3001 cgcggtgtcc tcgcgctgct tcttcaggct ctgcaccgcc ttgagcgcat cgaccaggct
     3061 ttgctcgaac tgcgccagca gcttgccgcg gcgcacctgc ccgcactcgc ggctgttcca
     3121 tttgtcctgc gcggccttga gctcgggcgg gtccttgggc ggcggcggtg ccttgccctt
     3181 gaccggcttg ggcggcggca gcttggccgc gcgcgccgcg atcttgtcct tgatgatctg
     3241 cggatcgatg cagtcttcgg cgctggcgta ggccacatga tccttggcgt ccttggcatc
     3301 ctcggccgca ttgcgcgcct tcacgccgtt gagcgcattc tccgcgccgg gaaccgcctt
     3361 ctcggccagc gccagacggc cgtcgatggt ggtgaggttg tcgtcgcgca ccttctgcag
     3421 ttgggtgacg ctttcgtagg actgcagcat gtaattgtca taggccagct ggcgcgtgtg
     3481 gcgccgctgc tcggccgcgg cggcctcggc ctggcgctgc tcctcggcca gttccgcggc
     3541 ggtcttttcg cgcgccatgg tgcccaccac ctggccttgc gcattgagca cgtcgcggtc
     3601 ctgcttggcg tattccggcg gcacgctgtc gccgcaggcg cgcacaccgt ccttgtcggt
     3661 ccagcacacc agttgcttgc ccttggccgc gacatgcaat gcgactactg acagcacgca
     3721 tcccagcaga cccaggacga cagttttatt gcgcatgact tgctcccttc ccgcgcccga
     3781 gtttacgcca cgcaatgctg cgcctggtat gcacgcatgg ccgccagcgt ctgcgcgtcc
     3841 acatggccct gtcccaaata ggccagcaca tccctgagcg tgaccaatgg aatcacgcgc
     3901 acgccggaat cctgctccat ctcctgcacc gccgaacggc cgctggcgcc tttctcctgg
     3961 cggtccagcg cgatgatcgc cgccaccggc tgggccccgg ctgaacgcag cagcgccacc
     4021 gcctcgcgca gcgcggtgcc ggcggtgagc acgtcatcga cgatgaccac gcgtcctttc
     4081 ggcggcgcac cgaccagggt gccgccctcg ccatgatcct tggcttcctt gcggttgtag
     4141 gcgtacggca cgtcgcgccc cgcctcggcc agcgccacgg cgacggtggt caccagcgga
     4201 atgcccttgt aggccgggcc gaacagcatg tcgtattcaa cccccgacgc cgccagcgcc
     4261 tgcgcatagg cgcgccccag ctcgcgcatg gccgcgccgc tggagatttt tcccaggttg
     4321 aagaaatacg gactctgccg cccggacttg agggtgaact ggccgaacct gagcacctcg
     4381 ctggacagcg ccagcgacag gaacgcggat tgataagact gcatcgtgcc cttgtgtcat
     4441 tctttcgcgg cgcttcttgt accactttca tccatgcgaa tcatcacgct taacgtcaac
     4501 ggcatccgct cggctgcgcg caagggcttg tttgactggc tcggcaagca aaaggcggat
     4561 ttcctgtgcc tgcaggagat caagtgccac gccagcgaca tcgagggcga tgagctgtac
     4621 tcgcccgcgg gctggcacgc gcatttcaac catgcgctga agaagggcta cgccggcacc
     4681 gcgctgttcg cacgcgagaa gccgcaggag atcctcgacc gcctcggcga ccaggaattc
     4741 gacgaccatg gccgctacgt ggaactgcgc tacggcaatc tctcggtggt ttcgctttac
     4801 gtgccgtcgg gttcgtccgg ccccgaggcg caggcgcgca aggatcgctt cctggaattc
     4861 ttcctgccga aactgcgcga gtggaaggcc agcggccgcg actacgtgat ctgcggcgac
     4921 tggaacatcg cgcacaagaa catcgacctg aagaactggc gcggcaacca gaagaattcg
     4981 ggcttcctgc cgcacgagcg cgcctggctc gaccgcgtgt tcggcgaggc gggctttgcc
     5041 gacgcctatc gcacgctgca tcccggactg gaaggcgaga gctacacctg gtggagcaac
     5101 cgcggggccg cctgggacaa gaacgtgggc tggcgcatcg actatcacat cgtcacgccc
     5161 gccatggcca aggcggtgaa gcgcgcgaag atctacaagg acgagcgctt ttccgatcac
     5221 gcgccgctga tcctggacta cgccgcttga gcggcacggc cgcctcgcgc ttcgatgcgc
     5281 tgcatgtcta tgcgcatccg cgcgtacgcg gcatgctgtt cctgggcttc tccgccggcc
     5341 tgccgttcct gctggtgttc tccacgctgt cggcctggct gacgcaggcg ggcatcgcac
     5401 gcgccaccat cggcatgctc agctggatcg gcatcagcta ttcgatcaag tttttctggt
     5461 cgccggtggt ggaccgcgcg cgcctgccgc tgctcacgaa ctggctcggg cggcggcgca
     5521 gctggatgct gctggggcag tgcggcatcg ccgccgggct atgcggcatc gccctgcacg
     5581 acccggcggc cgatctagaa gccgtggtct ggtgcgcgct gctggtcgcc ttctcgtcat
     5641 cgacgcagga catcgccatc gacgcctggc gcatcgaagc ggcgccggca tcgatgcagg
     5701 gcgccatggc ggcggcatac cagttcggct atcgcatcgc catgctcgcg gccagcgcgg
     5761 gcgccttgtg gctggccggc gagtacggct ggcacctgtc gtatgcggtg atggccgtcc
     5821 tggtcgcggt cggcctgtgc acgacgctgc tggtgggcga acctcaggcg caggtcagcc
     5881 gcgacacgct gatgcgcgag gcgcgcgtgc tggaattcct ggaaacgcgt gcgcactggc
     5941 cggagcagtt gcgccgcgcg ggcggctgga tcatcggcgc ggtggtgtgc ccgttcgtcg
     6001 acttcttcgc gcgcaacggc gctgcgcgcg gcgcactgat cctggctctg gtcggcacct
     6061 tctacctgcc gtacatcacc atgggggtga tgaccaatcc gttctacctc gacctgggat
     6121 tttcactgca gcagatcgca gcggtggcca agggctacgg cattgttgcc tcgctgctcg
     6181 gcgcgtttct cggcggcatc ggcgtggtgc gcctgggagc actgcgatgc atggccatcg
     6241 gcgccctgct ggtgatgttt gccaatctca cttactccgc gctggcgttg ctgcaccagc
     6301 cgacgctcgc cggcctcgcg ctgatcgtca gcgtggacaa catcgcctac aacttcgccg
     6361 cggcggcgtt cgtggcctac ctgtcgggtc tgaccaacgt gtcgtacacg gcaacgcagt
     6421 acgcgctgtt ctcatcgctg ttcaacctgc cgggaaagat catcgccggc ggctcgggcg
     6481 cactggtcga tgcgctgggt tatgcgccgt tcttcatcta caccgcgctg ctgggcatcc
     6541 cggcattgct ggtgatcgct gccatcacgc ggaaaccgac tccgcccgcc gcagccgcct
     6601 gaaggtttca gcaaagcgcc ggccgatgtc gttgccgcgc ggcacatccc acgcccggca
     6661 atacaccggc atggacagtc gatcgatgtt gtcggctcgg ccggcgtggc gcgcgccgag
     6721 cagcttcagg tgtttcagga tgcggtcgtt gtcgctggcc acgtcgtagt acagcgtgcg
     6781 gatgccgctg gcatgcgcag tcaggtgcag gcaggcctgc agcacgaaac cggcgccgcg
     6841 tccctggtaa tcgtcggcaa tggtgatctg cacatcggcc gcacgcggct cgccgttgac
     6901 gcggtgatag cgcgcgatgc cgatgcccgg ctcgtcgggc ttctccaggt tggccgcgcc
     6961 gaaggctgcg cggtcgcgca tgtccagcgt ggtcagctcg cgcagctgct cgtcggagag
     7021 ttcgttgatg tcgaggccgc ggcgccggcg cgccagctgc gacagctggc ggaaaccttc
     7081 gcgcaaccgc ggcgcgtcgt cgggcttgag cggccgcacc aggatcggcg tgccgtcgtc
     7141 cagcgcgtac ctgaaggcgg gatgcagctg cggcgtggcg aaccagctca gcacgagtca
     7201 gctcccgcgc gcagttcagt agagttcctc gccaggccgg tcgagaaaat ccagcggatt
     7261 gccgttgccg cgtgcgatcg attcttcggg atcgaagcca tagtcgttct tgtccaggtc
     7321 cagcgcgccg gagtaatcct cgggcggcgg tatgggcctg gcggcgagtt cgacccaggt
     7381 gtcgaaatcg aattcctcga gcgtgccgtc gaagtgctgc accagcacga tctccgcctt
     7441 ggcgtcgatg ccgacgatct cgaacggctc gccggcaacg tcgtaccact cgccgaccga
     7501 catgtcccgc agccattgcg ccatgagcca cctccgcgca gccatccttg gccgtattgc
     7561 acggctgatg ttctacgccg gtttgcgcag tgcttcaagc gcgcgcatga actgcgcgga
     7621 gcaactgact gagcgggcgc aaaagaaatc cgcgcgcctc atgggcacgg gatggtatgg
     7681 cggcgatgga tggcctcgat cgccttcagc agctcatcgt tgagcccgac gtcgatgctc
     7741 gcgatgttca gcttgagctg ctccatcgag gtggcgccga tcagcgtgca ggcgacgaac
     7801 ggccgcgagt ggacgaaggc cagcgccatc tgcgccgggt caagcccgtg ccgctgcgcc
     7861 agcgccacgt attcgcgcgt ggccagctca gcctcttcgt tgagatagcg cgtgaagcgc
     7921 gtgaacaggc tcatgcgcgc gccggccggg cgcgcgccgt gcaggtattt gccggccagc
     7981 acgcccatgg ccagcggcga atacgcgagc aatggcaggt gctcgcggtg cgcgaattcg
     8041 gccagcccca cttcgaagct gcggttgagc agcgagtacg gattctggat ggaggcgatg
     8101 cgcggcaggt ttttctcgcg cgccaggcgc aggtattcgc cgacacccca tggcgtttcg
     8161 ttcgacacgc cgacgtgccg caccttgccg gcgcgcacca gctcggccag cgccgccagg
     8221 gtttcctcga ccggcgtggc gtgcgcttct tcgcgcgcgg cgtagcccag cttgccgaag
     8281 tagttggtgt tgcgttcggg ccagtgcacc tggtacaggt cgatcacgtc ggtctgcagc
     8341 cgccggagcg aaccctcgca ggccgccagc accgacttgc ggtccaggtg cgtgccgccg
     8401 cgcaggtagg gaaagtcgcc cgggccggtg atcttggtgg ccagcacgat gcgcttgcgc
     8461 gcgccgctct tctgcagcca gctgccgatg tactgctcgg tgcggccctg cgtctcgcgc
     8521 cgcggcggca ccgggtacat ctcggccgca tcaaacaggt tcacgccgtg cgccagcgcg
     8581 taatccatct gcgcgtgcgc ttcggcctcg ctgttctgct cgccccaggt catggtaccc
     8641 aggccaacca cgcccagttc gggcaggccg tcgatcagcc ggcggtattt catgcggctt
     8701 gcgcccggcg cgcgatcagc tgttc
//
LOCUS       DASZZC010000011         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_129252,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000011 DASZZC010000000
VERSION     DASZZC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129252"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VHE37_00275"
     CDS             <1..957
                     /locus_tag="VHE37_00275"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT33987.1"
                     /translation="GKEVYADLVKLQTTMNLTPEQVHAAGHARMARIEAQMAEVRKTL
                     GFKGTAAEFAVHLRQQPGAVAKKPEDIGEKMRKHKNRIEARFDEFFAQRSRYDYDLVR
                     LHESVEAGMTWGYYGPPSAGDNRGLYHYNGSRLDSQAVIGAASLVFHELVPGHHLHIT
                     LQNENDNLPPFRKKALVNAFNEGWAEYAATLTGEMGLYDDPYDRYGRLVFDAFLTSRL
                     VVDTGMNTLGWSWEQAKQYMREHTLCAEGEIVSDTLRYACGIPAQALAYKLGDEEIIR
                     LRQKVKDRLKDKFSFRDFHSAVLGAGALPLPVLEWHLERVFS"
     gene            complement(1142..2125)
                     /gene="glk"
                     /locus_tag="VHE37_00280"
     CDS             complement(1142..2125)
                     /gene="glk"
                     /locus_tag="VHE37_00280"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HVT33988.1"
                     /translation="MYTSSSKLALVGDIGGSHARFALARLAGSVPAIEAVEQLSTKDY
                     ATLADAARHYLQRLPRESAPLRAVLAVASPVTGDQIKITNNPWSFSVAQLARELGFAS
                     VEVINDFAAISLALPLLGAADLKVIGPELPAPPKADRTYAVVGPGTGLGVSGLTVRGG
                     RSAVIESEGGHIGYAPVTPHEIDILRELTARFGRVSAERLASGTGVVNLYQAVCALQG
                     VPAPHAEAAAIAAEAAADANSVSARVLRLFCEILGGFAGDIALSYGAWDGVFLAGGVA
                     QKLCSWIEQGGFRERFEAKGRHQGLMQSVPTWLIVNPHVGLLGAAAAVGSE"
     gene            2471..>4542
                     /locus_tag="VHE37_00285"
     CDS             2471..>4542
                     /locus_tag="VHE37_00285"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HVT33989.1"
                     /translation="MRPIQKLLVANRSEIAIRVMRAAAELGVRTVAIYAHEDRFALHR
                     FKADESYLVGSGKKPIAAYLDIPGIIRIAREAGVDAIHPGYGFLSENPDFAQACADAG
                     ITFIGPTPAVMKTLGNKVAARNAAVAAGVPVMPATGPLPRDIAESKKLAAAVGYPLML
                     KASWGGGGRGMRVIETEADLAPAIDAARREADAAFGNDEVYLEKLVRRARHVEVQLLG
                     DMHGNLVHLYERDCSVQRRNQKVVERAPAPYLDAAARVQLCESALKLGRAVAYTHAGT
                     VEFLMDADSGKFYFIEVNPRIQVEHTVTEEVTGIDIVKAQIRVSQGAKIGATGSESAG
                     VPEQSAIRLNGHALQCRVTTEDPENGFTPDYGRLAVYRSAAGFGVRLDGGTAYAGAII
                     TPYYDSLLVKVTAWAPDAREAIQRMDRALREFRIRGLSTNLQFLENVINHPSFIGGDV
                     ITRFIDETPELFAFGKRRDRATRLLRFIGETTLHGNPEMKGRQAPAGELPPAILPAID
                     LNAPIPPGTRDTLRELGADKFAQWMKQQKQVLLTDTTLRDAHQSLFATRMRTIDMVAS
                     APYYARMLPHLLSLECWGGATFDVALRFLKEDPWDRLADLRERIPNIPFQMLLRASNA
                     VGYTNYPDNVVKFFVRHAAKSGIDIFRVFDSLNWVDNMRVAMDAVIESGAICEGTICY
                     TGDLFDASR"
BASE COUNT          808 a         1555 c         1466 g          713 t
ORIGIN      
        1 ggcaaggagg tctacgccga cctggtgaag ctgcagacga ccatgaacct caccccggag
       61 caggtccacg ccgcaggcca tgcacgcatg gcgcgtatcg aagcgcagat ggccgaggtg
      121 cggaagacac tcggcttcaa aggaacggct gcggagttcg ccgtgcacct gcgccagcag
      181 ccgggcgcgg tcgcgaagaa acccgaggac atcggcgaga agatgcgcaa gcacaagaac
      241 cgcatcgaag cgcgcttcga cgagttcttc gcgcagcgct ccaggtacga ctacgacctg
      301 gtgcggctgc atgaatcggt cgaggccggc atgacctggg gctactacgg cccgccgtct
      361 gccggtgaca accgcggcct gtaccactac aacggctcgc gcctcgacag tcaggcggtg
      421 atcggcgctg cctcactggt gttccacgag ctggtgcccg gccaccacct gcacatcacc
      481 ctgcagaatg aaaacgacaa cctgccgccg ttccgcaaga aggcgctggt caacgcattc
      541 aacgaaggct gggccgaata cgccgccacg ctgaccggcg agatgggcct gtatgacgac
      601 ccgtacgacc gctacggccg cctcgtcttc gatgcgttcc tgacctcgcg gctggtggtc
      661 gacaccggca tgaacacgct cggctggagc tgggagcagg ccaagcaata catgcgcgag
      721 cacaccctgt gtgcagaggg cgagatcgtc tcggacacgc tgcgctatgc ctgcggaatt
      781 ccggcacagg cgctggcgta caagctgggt gacgaggaga tcatccgcct gcgccagaag
      841 gtgaaagacc ggctgaagga caagttcagc ttccgcgact tccatagcgc ggtactcggc
      901 gccggcgcgc tgccgctgcc ggtgctggag tggcacctgg agcgcgtttt cagctgagga
      961 taaccctcgg gcagcaagat ggtccttgag cccattgagt ttctgaaaag cagccgctca
     1021 ggactgtcag cttttggccg acctgagacg aagaccgggc actaacggcc agaggttccg
     1081 ctccggtgcc tgcccaattt gcatccagtc ggcatttcaa ccgccgatgc aggcaccctg
     1141 actattcact gcccaccgcc gccgccgcgc cgagcaggcc gacatgcgga ttgacgatca
     1201 gccaggtcgg cacgctctgc atcaggccct ggtgacggcc cttggcctcg aagcgctcgc
     1261 ggaagccgcc ttgttcgatc cagctgcaca gtttctgcgc gacgccgccg gcgaggaaca
     1321 cgccatccca cgcgccgtag gacagcgcga tatcgccggc aaaaccgccg aggatttcgc
     1381 aaaacaggcg cagcacgcgc gcgctcacgc tgttggcatc ggcagcggcc tcggcggcga
     1441 tcgcagccgc ttcagcatgc ggcgccggca cgccttggag cgcgcacacc gcctggtaga
     1501 gattgaccac gccggtgccg gaggcgagcc gctcggcgga cacgcggccg aagcgcgccg
     1561 tgagttcgcg caggatgtcg atctcatgcg gcgtcaccgg cgcgtagccg atgtgcccgc
     1621 cctcggattc gatcaccgcg ctgcgtccac cgcgcacggt cagcccgctc acgcccaggc
     1681 cagtgccggg gccgaccacg gcgtaggtgc ggtccgcctt gggcggcgcc ggaagttcag
     1741 ggccgatcac cttgagatcg gcagcgccga gcagcggcag cgccaggctg atggcggcaa
     1801 agtcgttgat cacttccacc gaggcaaaac cgagctcgcg cgccagctgc gccacggaaa
     1861 aactccacgg gttgttggtg atcttgatct ggtcgccggt caccggcgag gccaccgcca
     1921 gcactgcgcg cagcggcgcc gattcccgcg gcaggcgctg caggtaatgg cgcgcggcgt
     1981 cggccagcgt ggcgtagtcc ttggtcgaca gctgctccac cgcctcgatc gccggcacgc
     2041 tgccggcgag gcgggccagc gcgaagcgcg cgtggctgcc gccgatgtcg ccaaccagcg
     2101 ccagcttgct gctcgatgtg tacatgcgct acgtctccag tccggccgcg cccctggatg
     2161 cggcgaaaag ccctacaccg caccggcatt ttgcctgtta ataattattg tctgattata
     2221 ttttcagtat atcttttata acggccaaca gggcgatgcg gtgaggccag ttcgccggct
     2281 gtttccatat attgggatca tattttacat gatgggatta aactggcgcg ccgtgttccg
     2341 tccgccgccc cgtcccgcct cctgctccat ctgacaccgg atttgcgcgc gcgtgcccct
     2401 ctcttccggg agtgcctgcg gccgcgacga tccggctaag ctggcgtgct tgctgctaga
     2461 gaattgtccc atgcgcccga tccagaagct gctggttgcc aaccgttccg agatcgccat
     2521 ccgcgtgatg cgcgcggcgg cggagctggg cgtgcgcacg gtggccatct acgcgcacga
     2581 agaccgcttc gcgctgcatc gcttcaaggc cgacgagagc tacctggttg gctcgggcaa
     2641 gaaacccatc gcggcctatc tcgacatccc cggcatcatc cgcatcgcgc gcgaagcggg
     2701 cgtcgatgcg atccatccgg gctatggatt cctgtcggag aacccggact tcgcgcaggc
     2761 ctgcgccgat gccggcatca cgttcatcgg cccgacgccg gcggtgatga agacgctcgg
     2821 caacaaggtc gcggcgcgca acgccgcggt cgccgccggc gtgccggtga tgccggccac
     2881 cgggccgctg ccgcgcgaca tcgcggagtc gaagaagctc gccgcggccg tcggctaccc
     2941 gctgatgctc aaggccagct ggggcggcgg cggacgcggc atgcgcgtga tcgagaccga
     3001 ggccgatctt gcgcccgcca tcgacgccgc gcgccgcgaa gccgacgccg cgttcggcaa
     3061 cgacgaggtg tacctggaaa aactggtgcg ccgcgcgcgg cacgtggagg tgcagctgct
     3121 cggcgacatg cacggcaatc tggtgcacct gtacgagcgt gattgctcgg tgcagcggcg
     3181 gaaccagaaa gtcgtggagc gcgcgccggc gccgtatctc gatgccgccg cgcgcgtaca
     3241 gctgtgcgag agcgcgctga agctcggccg cgcggtggcc tacacgcacg cgggcaccgt
     3301 cgaattcctg atggatgccg acagcggcaa gttctacttc atcgaggtca acccgcgcat
     3361 ccaggtggaa cacacggtga ccgaggaggt caccggcatc gacatcgtca aggcgcagat
     3421 ccgcgtgagc cagggcgcga agatcggcgc caccggcagc gagtccgccg gcgtgcccga
     3481 gcaatccgcc atccgcctca acggccacgc gctgcaatgc cgcgtgacca ccgaggaccc
     3541 ggagaacggc ttcacgccgg actacggccg cctcgcggtg tatcgcagcg ccgccggttt
     3601 cggcgtgcgc ctcgacggcg gcaccgctta tgccggcgcc atcatcacgc cgtattacga
     3661 ctcgctgctg gtgaaggtga cggcctgggc gccggatgcg cgcgaggcga tccagcgcat
     3721 ggaccgcgcg ctgcgcgagt tccgcatccg cgggctgtcg accaatctcc agttcctgga
     3781 aaacgtcatc aaccatccgt cgttcatcgg cggcgacgtg atcacccgct tcatcgacga
     3841 gacgcccgaa ctgttcgcct tcggcaagcg ccgcgaccgc gccacgcgcc tgctgcgctt
     3901 catcggcgag accacgctgc acggcaaccc ggagatgaag ggccgccagg cgcccgcggg
     3961 cgaactgccc ccggccatcc tgccagccat cgatctcaac gcacccatcc cgcctggcac
     4021 gcgcgacacg ctgcgcgaac tcggcgcgga caaattcgcg cagtggatga agcagcagaa
     4081 acaggtgctg ctcaccgaca ccacgctgcg cgatgcgcac cagagcctgt tcgccacgcg
     4141 catgcgcacc atcgacatgg tggcgagcgc gccgtactac gcgcgcatgc tgccgcacct
     4201 gctgtcactg gaatgctggg gtggcgccac cttcgacgtg gcgctgcgct tcctcaagga
     4261 agatccgtgg gatcgcctcg ccgacctgcg agaacgcatt ccgaatattc cgttccagat
     4321 gctgctgcgc gcgagcaacg ccgtcggcta caccaactac cccgacaacg tggtgaagtt
     4381 cttcgtgcgg catgcggcga aaagcggcat cgacatcttc cgcgtgttcg actcgctgaa
     4441 ctgggtcgac aacatgcgcg tggcgatgga cgccgtcatc gaatccggcg cgatctgcga
     4501 aggcaccatc tgctacacgg gcgatctctt cgacgcgtca cg
//
LOCUS       DASZZC010000012         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_707430,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000012 DASZZC010000000
VERSION     DASZZC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707430"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1027)
                     /locus_tag="VHE37_00290"
     CDS             complement(284..1027)
                     /locus_tag="VHE37_00290"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HVT33990.1"
                     /translation="MPDSTLAPPPGYTTIAPLDKQKHAGLGLKPGIGYAWAAQLNAVF
                     IGAAEMPRAALDFPIAFVRDQATGEFVPVSVLGLKQNQNLFVDAGGRWREHHYVPAYL
                     RRYPFCLADIPSPGGQEARHLICVDESQLVAGAPPLFDAAGQPTAAWEPMRQLIEALE
                     TARQQTRALCKRLEALQLLVPFDAVAVPRAGERMRLAGMFRIDEEKLALIPLRDLRMM
                     LKRAEMRAVYAHLLSLENFAKLMDLAVAR"
     gene            complement(1085..1921)
                     /locus_tag="VHE37_00295"
     CDS             complement(1085..1921)
                     /locus_tag="VHE37_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33991.1"
                     /translation="MHGATRVLIALLLSLGTYGGTALADAGGLAGQVTLMTGRGTASA
                     ADGAVRSLTTGDSVYSGEVISSGANSYVSLKFVDGGLVLLRPRTRFEIQDYSYTPPAT
                     AAPMAATPTPIAPGTIGGAKPAIPAAPSVAAPPAQASSGDEGNGTTRAFFRLLKGSFR
                     AVSGLIGKANQDDYRVSTPVATIGIRGTDYFALIIDAAYAKDPVLKSSLPPGISSEGG
                     LYVEQYEGSTVVTNNAGQQTVVSMGQHLVTLGNGQQLHVKQPKLLTSDPPPDPKQMKC
                     NH"
     gene            complement(1935..>2438)
                     /locus_tag="VHE37_00300"
     CDS             complement(1935..>2438)
                     /locus_tag="VHE37_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT33992.1"
                     /translation="GSGLKQSQTGGSFTFGSLGFNGSLLLNYTPSNPNVDMTLDPQLA
                     TALPSGMTLALGSSNYLGNITVATAANPITVQNVVNFAFVTKGAVSGAEFLKTNGRVL
                     VLSGVAGGGAAQDQANQSINNVNGLDQLTPDYGQVEDPASAAGALQNPPIEFNNQPGD
                     SQTFECT"
BASE COUNT          381 a          778 c          862 g          417 t
ORIGIN      
        1 agccgggccg gatgtccact ggacatccgg caagtcccgc ctcgccccag gccagcacag
       61 cggttcggct caagccgggt ctcctggagt gtccctgcgg cagggtgata ccggcgcaag
      121 tctccgcgtg ctacgaattt cgtagcagga ggagcaggcg aacttgttgt aggagcgcat
      181 ctggcttgac cgcacccggc agggcgttgt tataaaaact acatctaaat taatttaaca
      241 caataaataa taacagtcgc aaggacggtg cggtggcagg ttttcagcgc gccacggcca
      301 ggtccatcag cttggcgaag ttctccagcg acagcaggtg cgcgtacacc gcgcgcatct
      361 cggcgcgctt gagcatcatg cgcaggtcgc gcagcgggat cagcgccagc ttctcctcgt
      421 cgatgcggaa catgccggcc aggcgcatgc gctcgccggc gcgcggcacg gcgacggcat
      481 cgaacggcac cagcagctgc agcgcttcca gccgcttgca cagcgcgcgc gtctgctggc
      541 gcgcggtttc cagcgcctcg atcagctggc gcatcggctc ccatgcggcg gtcggctggc
      601 cggcggcgtc gaacagcggc ggcgcgccgg caaccagctg gctttcgtcg acgcagatca
      661 ggtggcgtgc ttcctggccg ccgggcgagg gaatgtcggc caggcagaac gggtagcgcc
      721 ggaggtacgc gggcacgtag tggtgctcgc gccagcgccc gccggcatcg acgaacaggt
      781 tctggttctg cttgaggccg agcacggaca ccggcacgaa ctcgccggtg gcctggtcgc
      841 gcacgaaggc gatcgggaaa tccagcgcgg cgcgcggcat ctcggcggcg ccgatgaaca
      901 cggcattcaa ttgcgcggcc caggcgtagc cgatgccggg cttcagtccg agcccggcgt
      961 gcttctgctt gtccagcggt gcaatggtgg tgtagccggg cggcggtgcg agcgtggaat
     1021 caggcatggc gatggacggt ggaaatgaaa accgccccgg gcggggcggt cggcgcttcg
     1081 atttctagtg gttgcacttc atctgcttgg gatcgggcgg cggatcgctg gtgagcagct
     1141 tgggctgctt gacgtgcagc tgctggccgt tgcccagcgt gaccaggtgc tggcccatgg
     1201 acactacggt ctgctggccg gcgttgttgg tcaccaccgt gctgccttcg tactgctcca
     1261 catagaggcc gccttccgac gagatgcccg gcggcaacga ggatttcagc accgggtctt
     1321 tcgcatacgc cgcgtcgatg atcagcgcga agtaatcggt gccgcgaata ccaatcgtcg
     1381 ccaccggcgt ggacacgcgg tagtcgtcct ggttggcctt gccgatcagg ccggacaccg
     1441 cgcggaagct gcccttgagc aggcggaaga aggcgcgcgt ggtgccgttg ccctcgtcgc
     1501 cggaactggc ctgcgccggc ggagcggcca ccgaaggcgc tgccgggatc gccggcttgg
     1561 cgccgccgat ggtgcccggc gcgatcggcg tcggcgtggc ggccatcgga gcggccgtcg
     1621 ccggcggcgt gtagctgtag tcctggatct cgaagcgcgt gcgcggccgc agcagcacca
     1681 gcccgccatc gacgaacttc aggctgacgt agctgttggc gccggagctg atcacctcgc
     1741 cggaatagac cgaatcgccg gtggtgagac tgcgcaccgc gccgtcggcg gcgctggcgg
     1801 tgccgcgccc ggtcatcagc gtgacctggc cggcgagccc gccggcgtcg gccagcgccg
     1861 tgccgccata agtgcccagt gacagcagaa gggcgatcaa tacccgtgtt gcgccatgca
     1921 tggtgctcgc tcccttaggt gcactcgaag gtctggctgt cgcccggctg gttgttgaac
     1981 tcgatgggcg gattctgcag cgcgccggcg gcactcgccg gatcttccac ctggccgtag
     2041 tccggcgtga gctggtcgag gccattgacg ttgttgatcg actggttggc ctggtcctgc
     2101 gcggcgccgc cgccggccac gccggaaagg accagcacgc ggccattggt cttgaggaac
     2161 tccgccccgc tcaccgcgcc cttggtgacg aaggcgaagt tcaccacgtt ctgcacggtg
     2221 atcggattgg cggccgtggc cacggtgatg ttgcccaggt aattgctcga acccagcgcc
     2281 agcgtcatgc cgctgggcag ggccgtcgcc agctgcgggt ccagcgtcat gtcgacgttc
     2341 ggattcgatg gcgtgtagtt gagcagcagc gaaccgttga agccgagcga gccgaaggtg
     2401 aagctgccgc cggtctgcga ttgcttgagc ccgctgcc
//
LOCUS       DASZZC010000013         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_707432,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000013 DASZZC010000000
VERSION     DASZZC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707432"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VHE37_00305"
     CDS             <1..530
                     /locus_tag="VHE37_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HVT33993.1"
                     /translation="GKIIHDPRLTWNTIDVVRAAGGQPVLCKTGHAFIKERMRKENAI
                     YGGEMSAHHYFRDFAYCDNGNIPWLLVASLISESGLTLSQLVAERMKKYPASGEINRK
                     VADVKATIARVEANYRDGAIDRTDGISVEFNDWRFNLRGSNTEPVLRLNVESRGDEKL
                     MQQKTAEILALIDRG"
     gene            complement(534..1202)
                     /locus_tag="VHE37_00310"
     CDS             complement(534..1202)
                     /locus_tag="VHE37_00310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVT33994.1"
                     /translation="MLFALCASVLLTSAAASSSIPGIAPGSLAPEAAGEVLQGAPETK
                     LSILRGKVVVLDFWASWCGPCVQSMPELDAIYRELQPQVAAGRFAMLGVSIDDDAALA
                     RRFLALHPVSYPIVDDMAGIAKQTYGIWRLPATMLIEPDGRVHFIYWGSHEGYGAEVK
                     QKVLDLLAEIRWKEAHGGGAAQMSPVGGAPKFDPVPRAAPLMDPLPQPAAQQPMPLSP
                     APGQ"
     gene            complement(1219..1707)
                     /locus_tag="VHE37_00315"
     CDS             complement(1219..1707)
                     /locus_tag="VHE37_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HVT33995.1"
                     /translation="MQPTMLNHNLCPCCGQPRRLAHLLDLYDRNYQLLQRLIPELDMP
                     FERAVSKSSSDLPLHLEVMERDRYTATIKLTYEFTDTDGVRRDPDLWVRVYRDARVCE
                     ALKCAFRPPWMAEEGGDPEAGRYLSDQWGRNLMLGKWLEYLLEHGHGFGMAARPRVKK
                     AA"
     gene            1816..>2227
                     /locus_tag="VHE37_00320"
     CDS             1816..>2227
                     /locus_tag="VHE37_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HVT33996.1"
                     /translation="MAIREISTKPYLDQKSGTAGLRKKVAVFQQPHYLENFLQSVFDC
                     APELKGKSLVIGGDGRYHNRAAIQTAIAMAAANGVTRVIVGQGGLLSTPAVSNLIRKR
                     GAAGGMVFTASHNPAGPDGDFGIKFNVAGGGQAGE"
BASE COUNT          426 a          711 c          737 g          353 t
ORIGIN      
        1 ggggcaagat cattcacgac ccgcgcctca cctggaacac catcgacgtg gtgcgtgccg
       61 ccggcggcca gccggtgctg tgcaagaccg gccatgcttt catcaaggag cgcatgcgca
      121 aggagaacgc catctacggc ggcgagatga gcgcgcacca ctacttccgc gatttcgcct
      181 actgcgacaa cggcaacatc ccgtggctgc tggtggcatc gctgatcagc gagagcgggc
      241 tgacgctgtc gcaactggtg gccgagcgca tgaagaaata cccggccagc ggcgagatca
      301 accgcaaggt tgccgacgtg aaggcgacca tcgcgcgggt cgaggcgaac taccgcgacg
      361 gcgcgatcga ccgcaccgac ggcatcagcg tggagttcaa cgactggcgc ttcaacctgc
      421 gcggctccaa caccgagccg gtgctgcggc tgaacgtgga atcgcgcggc gacgagaagc
      481 tgatgcagca gaagacggcg gaaatcctgg cgctgatcga tcgcggctga agcctactgg
      541 cccggcgcag gcgacaacgg catcggctgt tgcgctgcag gttgcggcaa cgggtccatc
      601 aatggcgcgg cgcgcggcac cgggtcgaac ttcggtgcgc cgccgaccgg cgacatctgc
      661 gccgcgccgc caccatgcgc ttccttccag cggatttccg ccagcaggtc gagcactttc
      721 tgcttcacct cggcgccgta gccttcatgc gagccccagt agatgaagtg cacgcggcca
      781 tcgggttcga tcagcatggt cgccggcagg cgccagatgc cataggtctg cttggcaatg
      841 ccagccatgt catcgacgat cgggtagctc accggatgca gcgccaggaa gcgccgggca
      901 agcgccgcgt cgtcatcgat cgacacgccg agcatggcga agcgccctgc ggccacctgc
      961 ggctgcagtt cgcggtagat ggcgtcgagc tcgggcatcg actgcacaca gggcccgcac
     1021 cagctggccc agaaatccag caccaccacc ttgccgcgca ggatggaaag cttggtctcg
     1081 ggcgcgccct gcagcacttc gcccgccgct tcaggcgcga gggaaccggg ggcgatgccg
     1141 ggaatggatg aactggcggc ggcgctggtc agcaggacgg aggcgcacag cgcaaaaagc
     1201 acgcgcaaat gtttttcctc aggcggcttt cttcacgcgt gggcgcgcgg ccatgccaaa
     1261 gccgtgaccg tgctccagca ggtattcgag ccatttgccg agcatcaggt tgcggcccca
     1321 ctggtcggac aggtagcgtc cggcctcggg atcgccgccc tcttccgcca tccatggcgg
     1381 gcggaaggcg cacttgagcg cttcgcacac gcgtgcgtca cggtatacgc gcacccacag
     1441 gtcgggatcg cgccgcacac cgtcggtgtc ggtgaactcg taggtcagct tgatggtcgc
     1501 tgtgtagcgg tcgcgctcca tcacttcgag gtgcagcggc aggtcgctgg aactcttgga
     1561 cacggcgcgc tcgaacggca tgtcgagttc cgggatcagg cgctgcagca gctggtaatt
     1621 gcggtcgtac aggtccagca ggtgcgccaa tcgccgcggc tggccgcagc aagggcaaag
     1681 attgtgattg agcatcgtcg gttgcatatg ggagcgcact ataaatgaag gcttgtgacc
     1741 tgaatatgct gcacaagttc tgggcgaagg ggtaaattta aagtcccgcg gcgtttagcc
     1801 ttagagaaca agaacatggc cattcgagaa atctccacca aaccctacct cgaccagaag
     1861 tcgggaaccg ccgggctgcg caagaaagtg gccgttttcc agcagccgca ctacctggaa
     1921 aatttcctgc agtcggtctt cgactgcgcg cccgagctga aaggcaaatc gctggtgatc
     1981 ggcggcgacg gccgctacca caaccgcgcg gcgatccaga ccgccattgc catggccgca
     2041 gccaatggcg tgacgcgggt gatcgtcggc cagggtggac tgctgtcgac gccggcggtg
     2101 tccaacctga tccgcaagcg cggggcggcc ggcggcatgg tgttcaccgc cagccacaac
     2161 cccgccggac ccgacggcga cttcggcatc aagttcaacg tggccggcgg cggccaggct
     2221 ggcgaag
//
LOCUS       DASZZC010000014         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_321720,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000014 DASZZC010000000
VERSION     DASZZC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321720"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /gene="rplJ"
                     /locus_tag="VHE37_00325"
     CDS             complement(<1..120)
                     /gene="rplJ"
                     /locus_tag="VHE37_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HVT33997.1"
                     /translation="MPLNLAQKKELVAELAEVAAKAHSLVAAEYAGLTVEQLTE"
     gene            complement(434..1141)
                     /gene="rplA"
                     /locus_tag="VHE37_00330"
     CDS             complement(434..1141)
                     /gene="rplA"
                     /locus_tag="VHE37_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HVT33998.1"
                     /translation="MALTKRFKAMAGQVEPGKQYPVDAALKILKDNAKTKFVESVDVA
                     IRLGIDAKKSDQGVRGSTVLPHGTGKTVRVAVFCPAGEKAEAAKAAGADAVGMDDLAE
                     KMQGGDIDFGVVIATPDAMRVVGKLGQLLGPRGLMPNPKVGTVTPDVVGAVKNAKAGQ
                     VRYRNDKAGIVHCSIGKVNFEIDALRDNLNALIADLLKVKPSTAKGQYLQKVSVSSTM
                     GLGVIVDPSTLAATATK"
     gene            complement(1143..1574)
                     /gene="rplK"
                     /locus_tag="VHE37_00335"
     CDS             complement(1143..1574)
                     /gene="rplK"
                     /locus_tag="VHE37_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HVT33999.1"
                     /translation="MAKKVVGYIKLQVKAGEANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQKIEKGLPIPVIITAYSDRSFTFVTKTPPATVLLKKAVGLQKGSPKPNTDKVGKVT
                     RKQLEDIAKQKEPDLTAADLDAAVRTIAGSARSMGLNVEGV"
     gene            complement(1695..2267)
                     /gene="nusG"
                     /locus_tag="VHE37_00340"
     CDS             complement(1695..2267)
                     /gene="nusG"
                     /locus_tag="VHE37_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HVT34000.1"
                     /translation="MSETTSKRWYVVHAYSGFEHQVARALIERVARSGMQEKFGEVLV
                     PTEEVVEMRGGQKRKSERKFFPGYVLVQIETTGEARAPRIDDECWHLVKETPKVMGFI
                     GGTADKPLPIKDSEADSILNRVKEGIEKPRPKVLFEPGEMVRVIDGPFNDFNGVVEEV
                     NYEKSRLRVAVLIFGRSTPVELEFGQVEKA"
     gene            complement(2271..2675)
                     /gene="secE"
                     /locus_tag="VHE37_00345"
     CDS             complement(2271..2675)
                     /gene="secE"
                     /locus_tag="VHE37_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HVT34001.1"
                     /translation="MPDNNKTMNAKAETIGTGKAADVGLLTLAVLIVIAGVAAYYVLP
                     ATVAPIWHTLSVVGAIVVAAGLSWFTTAGHAVRDYLRETQFEMRKVVWPTREETIRTT
                     LVVILVVVLLSVVLGLIDIILKSVILDWLLKL"
BASE COUNT          444 a          932 c          804 g          565 t
ORIGIN      
        1 ctcggtgagc tgctcgaccg tgagacccgc gtactccgcg gcgaccagcg agtgcgcctt
       61 ggcggccact tctgccagtt cggcaacgag ctctttcttt tgtgccagat tgagcggcat
      121 tgcttgcctc caatgaatcc atcgcgctcc gtgcgcgatg cattcgaacc aaacggaccg
      181 tcggcatcct gccgatccgc cggcacttcg cggtgcctga ttgcggtgtc cgctcggaga
      241 tcaaccttcc agtcgattcg ttgcgggcga caccatctgc gcaggcggga atccatccag
      301 cgattccgat tgaggcccgg cgggtcgaaa cccgcttcgc cacctgcggt ctttgacggc
      361 ttgccactca tgcagacgtg ggttcgccct caaaaccctg ttgccctctc cttccgggaa
      421 agggttcaga tcatcacttt gttgccgtgg ccgccagcgt ggacgggtcc acgatcacgc
      481 cgagacccat cgtgctggag accgacacct tctgcaggta ctggcccttg gcggtggacg
      541 gcttgacctt caaaaggtcg gcgatcagcg cgttcaagtt gtcgcgcagc gcgtcgattt
      601 cgaagttcac cttgccgatc gagcagtgca cgatgccggc cttgtcattg cggtaacgca
      661 cctggccggc tttggcgttc ttcaccgcgc cgaccacgtc cggcgtcacg gtgccgacct
      721 tggggttcgg catcaggcca cgcggaccca gcaactggcc gagcttgccg accacgcgca
      781 tcgcgtccgg ggtcgcgatc acgacaccga aatcgatgtc gccgccctgc atcttctcgg
      841 cgagatcatc catgccgacc gcatcggcgc cggcggcctt ggccgcctcg gccttctcgc
      901 cggccgggca gaacaccgca acgcgcaccg tcttgccggt gccgtgcggc agcacggtgg
      961 aaccgcgcac gccctggtcg gatttcttgg cgtcgatgcc cagccggatc gccacgtcca
     1021 ccgactccac gaacttggtc ttggcgttgt ccttcaggat cttcagcgcc gcgtcgaccg
     1081 gatactgctt gccgggttcg acctggccgg ccatcgcctt gaaacgtttg gtcagtgcca
     1141 tgtcagacgc cctccacgtt gagacccatg ctgcgcgcgc tgcctgcgat ggtgcgcacg
     1201 gctgcgtcca gatccgccgc ggtgaggtcg ggctccttct gcttggcgat gtcttccagc
     1261 tgcttgcgcg tcaccttgcc caccttgtcg gtgttcggct tgggcgaacc cttctgcagc
     1321 ccgaccgctt tcttcagcag cacggtggcg gggggggtct tggtgacgaa cgtgaagctg
     1381 cggtccgaat acgcggtgat gatcaccggg atcggcaggc ccttctcgat cttctgcgtg
     1441 gcggcattga acgccttgca gaactccatg atgttgaggc cgcgctggcc caacgccgga
     1501 ccgaccggcg ggctggggtt ggcctcaccg gccttgacct gcagcttgat gtaaccgacg
     1561 actttctttg ccattgcttt tgctcctgcg agttcaagcg cctgttgtca ggctcctcgc
     1621 tctgttgaac aagggattag gggcgagtag ccagggcgca tgaacgcagc accctcgcta
     1681 cccgtccctc gtccctacgc tttttccacc tgcccgaact ccaattccac cggagtcgaa
     1741 cgaccaaaaa tcagcaccgc cacgcgcagg cggctcttct cgtaattgac ttcctcgacc
     1801 acgccgttga agtcgttgaa tgggccatcg atgacgcgca ccatctcgcc cggctcgaac
     1861 aacaccttcg ggcgcggctt ctcgatgccc tccttgacgc gattcaagat cgaatcggcc
     1921 tcggaatcct tgatcggcaa cggcttgtcg gcggtgccgc cgatgaagcc catcaccttg
     1981 ggcgtctcct tcacgagatg ccagcactcg tcgtcgatgc gcggcgcgcg cgcttcgccg
     2041 gtggtctcga tctgcaccag cacgtagccc gggaagaact tgcgctcgct cttgcgcttc
     2101 tggccgccgc gcatctcgac cacttcttcg gtcggcacca gcacttcgcc gaacttttcc
     2161 tgcatgcccg aacgcgcgac gcgctcgatc agcgcgcgcg cgacctggtg ctcgaagccc
     2221 gagtaggcgt ggaccacgta ccagcgtttg cttgtcgttt cgctcatgtc ttaaagcttc
     2281 agcaaccagt cgaggatgac cgacttcagg atgatgtcga tcagacccag cacgacgctg
     2341 agcaggacga ccacgagaat cacgaccagg gtcgtgcgga tggtttcttc gcgcgtcggc
     2401 cacaccacct tgcgcatttc gaactgcgtt tcgcgcaggt aatcacgcac ggcatgcccg
     2461 gccgtggtga accaggacaa gccagcggcg accacgatcg cgccgaccac gctcagcgtg
     2521 tgccagatcg gcgcaacggt ggccggcagc acgtaatacg ccgccacacc ggcgatgacg
     2581 atcagcaccg ccagcgtcag caagccgaca tccgctgcct tgccggttcc gatcgtttcc
     2641 gctttcgcat tcatggtttt gttgttgtcc ggcactgctg tgttcgcatc agttcacgca
     2701 ttggcacgcc aggagggact cgaaccccca acctgcggtt ttgga
//
LOCUS       DASZZC010000015         4466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_65144,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000015 DASZZC010000000
VERSION     DASZZC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65144"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /locus_tag="VHE37_00350"
     CDS             complement(<1..1832)
                     /locus_tag="VHE37_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HVT34002.1"
                     /translation="MGLLHIVAVTLGILAVHLLQQLPPAWMLAALCLPALLPWRGRAL
                     WACFALGVLLTSWRAHALLDQRWPASKHDQQFTVQGHVASLPEISSRESGPDADLTAR
                     FLFEPEADEFPSRIRVSWYNLDAALTPPPALQAVSPSPASGRGALESLRDNQLRGGDC
                     WKLTLRLKTPHGSLSPGAFDYEGWLFRQGIGATATVQDAQRCDDTRGYVLLRLRQSLS
                     DHLREWLPHEPATGLIAALTFGDTSGLSDADWDVFRLTGTTHLVAISGFNVAIIAGVA
                     FFIFRWLWSLWPPLLLRLPAQKAGMLGAAVVALLYALLAGWQAPVARSVLMLWIILAA
                     AWLQRLAQPSRVLALAWLAILLIDPFEVMAPGLWLSFGAVAAIFFVTTNRREAPDPLR
                     AAIALQLMLSVALTPLTLWFFHGASWCAPLVNLLAVPLFSLMTPILLGALLLAAAWPA
                     VGVSVLAGCATVMQQTRDALGWIAAHVPHEWIAAAPPVPALLLALMGAALLYAPRGLP
                     LRVPALLCFLPLLFPPDTAPRSGFDAAVLDVGQGLSVVVRTAHHAMVFDTGPGFDEGY
                     NAGRSVVAPYLLTHGVRELDLMLISHADNDHSGGAPALRQLLEVR"
     gene            complement(1941..2627)
                     /gene="lolD"
                     /locus_tag="VHE37_00355"
     CDS             complement(1941..2627)
                     /gene="lolD"
                     /locus_tag="VHE37_00355"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HVT34003.1"
                     /translation="MSESILRCSALEKTYDDGRLKASVLRGVNLDIARGERVAIVGAS
                     GSGKSTLLHILGGLDAATTGEVYVNGEQMSALSDAARGELRNRSLGFVYQFHHLLPEF
                     SALENVAMPLRIRRMPLGQAYAKASELLARVGLAERLEHKPGELSGGERQRAAVARAL
                     VTRPACVLADEPTGNLDSGTAEKVFDLMLELNRELSTSLIVVTHDMKLAARMDRVLRL
                     DGGVLQVATP"
     gene            complement(2620..3867)
                     /locus_tag="VHE37_00360"
     CDS             complement(2620..3867)
                     /locus_tag="VHE37_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HVT34004.1"
                     /translation="MAAPYELFTGLRYTRAKRRNHFISFISLASMIGIAVGVMALITV
                     LSVMNGFERELRTRILGMASHATISAFEGGMSDWQDVAAKARANPAIEAVAPYIEGEG
                     MVKVGSDLSGTMLRGVLPEYEATVSDIGRTMKAGKLTDLKAGEFKIIIGRELAQALGV
                     IVGDKIDLMIPQASVTPAGVLPRFRRFTVAGIFQVGMYEFDRGLVLIHLDDAAALYRM
                     PGQVTGVRLKLHDLFQAPQVARELAGSLAGVYYVSDWTRNHANFFKAVATEKMVMFII
                     LSLIVGVAAFNIVSTLVMVVQDKQADIAILRTLGATPRSIMAIFMVQGSVIGVMGTFF
                     GVVLGVLLAENVQTLVPLLEAATGHQFLSPDIYYISDLPSELKLSDVTKISLLSLALG
                     FFSTLYPAYRASRMAPAEALRYE"
     gene            3956..>4466
                     /locus_tag="VHE37_00365"
     CDS             3956..>4466
                     /locus_tag="VHE37_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVT34005.1"
                     /translation="MPETAGHRYWGFLSYSHADEAAAVRLHRALESYVLPRRLRRAHG
                     LPRRLTPIFRDVDELEAASGLNTRLQDALDQSRWLILLCSPAAAASKYVNAEVEYFLQ
                     RHGAARVLCVLAGGEPPECFPPAIRALPEEPLAADLRPGRDAQTARLKLVAALAGVGF
                     TELRNREAQR"
BASE COUNT          774 a         1524 c         1541 g          627 t
ORIGIN      
        1 cgcacctcga gcagctgccg cagtgccggc gcgccgccgg agtgatcgtt gtcggcgtga
       61 gagatcagca tcaggtccag ttcgcgcacg ccgtgcgtga gcaggtacgg cgcgaccacc
      121 gagcgaccgg cgttgtagcc ctcatcgaag cccggtcccg tgtcgaacac catggcgtga
      181 tgcgccgtgc gcacgaccac ggacaggccc tgccccacgt cgagcaccgc cgcatcgaaa
      241 ccgctgcgcg gggcggtgtc cggcgggaac agcagcggca ggaagcacag cagcgccggc
      301 acgcgcagcg gcaggccgcg cggcgcgtac agcagcgcag cgcccatcag cgcgagcaaa
      361 agggccggca ccggcggtgc ggcggcgatc cattcgtgcg gcacgtgcgc ggcgatccag
      421 cccagcgcat cgcgcgtctg ctgcatcacc gtcgcgcagc cggccaggac cgacacgccg
      481 accgcaggcc aggccgcggc cagcagcagg gcgccgagca ggatgggcgt catcagcgag
      541 aacaatggca cggccagcag gttcaccagc ggcgcgcacc acgaggcgcc atggaagaac
      601 cacagcgtga gcggcgtcag cgccacgctg agcatcagct gcaacgcgat ggcggcgcgc
      661 agcggatcgg gtgcctcgcg gcggttggtg gtgacgaaga agatcgcggc aacggcaccg
      721 aacgacagcc acaggcccgg cgccatcacc tcgaacgggt cgatcagcag aattgcgagc
      781 cacgccagtg ccagcacgcg cgatggctgc gccagccgtt gcagccacgc cgcggcaagg
      841 atgatccaca gcatcagcac cgagcgcgcc accggcgcct gccagccggc gagcagcgcg
      901 tagagcaagg caaccaccgc ggcgccgagc atgcccgcct tctgcgccgg caagcgcagc
      961 agcagcggcg gccacagcga ccacagccag cgaaagatga agaaggcgac gccggcgatg
     1021 atggcgacgt tgaagcccga gatggcgacg aggtgcgtgg tgccggtcag ccggaacaca
     1081 tcccagtcgg cgtcggagag acccgaggtg tcgccaaagg tcagcgcggc gatcagcccg
     1141 gtcgccggct cgtgcggcag ccactcgcgc aggtgatcgg acaaggactg ccgcaagcgg
     1201 agcagcacgt agccgcgcgt atcgtcacag cgctgcgcgt cctgcaccgt ggccgtggca
     1261 ccgatgccct ggcggaacag ccagccctcg taatcgaacg cgccgggact gagcgagccg
     1321 tgcggcgtct tcaggcgcag cgtcagcttc cagcaatcgc cgccacggag ttggttatcc
     1381 cgcagcgatt ccagcgcccc tctaccgcta gcgggagagg gggaaaccgc ctgcagagca
     1441 ggcggtgggg tgagggcagc atccaggttg taccaggaca cgcggatgcg cgagggaaat
     1501 tcgtctgctt cgggctcgaa caggaagcgt gcggtgagat cggcatcggg tccgctctcg
     1561 cggctggaga tttccggcag cgacgcgaca tgcccctgca cggtgaattg ctgatcgtgc
     1621 ttcgacgccg gccagcgctg gtcgagcagc gcgtgcgcgc gccagctggt gagcaacacg
     1681 ccgagcgcga agcacgccca aagcgcgcgc ccgcgccagg gcagcagcgc gggcaggcac
     1741 agcgcagcga gcatccacgc cggcggcagc tgctgcagca ggtgaacggc gaggatgccg
     1801 agcgtcactg caacgatgtg gagcagcccc atctgctctc cccgatatcc gtgttcccgc
     1861 gaaggcggga acccagaact taccttcgtt cacccctgcg ctcaaaaact ggattcccgc
     1921 tttcgcggga atgacgagtt ctacggtgtc gcaacttgaa gcacgccccc gtcgagccgc
     1981 agcacgcggt ccatgcgcgc ggccagcttc atgtcgtgcg tcaccacgat caggctggtg
     2041 gacaactcgc ggttgagctc cagcatcagg tcgaagactt tttccgcagt gccgctgtcg
     2101 aggttgccgg tgggctcgtc ggccagcacg caggccgggc gcgtgaccag cgcacgcgcc
     2161 accgccgcgc gctggcgctc gccgccggag agttcgcccg gcttgtgttc caggcgctcg
     2221 gccagcccca cgcgcgccag cagttcggaa gcttttgcgt atgcctgccc gagcggcatg
     2281 cggcggatgc gcagcggcat cgccacgttt tccagcgcgg aaaattccgg cagcaggtga
     2341 tggaactggt agacgaagcc cagcgagcgg ttgcgcagtt cgccgcgcgc ggcgtcggac
     2401 agcgccgaca tctgctcgcc gttgacgtac acctcgccgg tggtcgcggc atcgagcccg
     2461 ccaaggatgt gcagcagcgt cgatttaccg ctgccgctgg cgccgacgat ggcaacgcgc
     2521 tcgccgcgtg cgatgtccag gttcacgccg cgcagcacgc tggccttgag ccggccgtcg
     2581 tcgtaggttt tttccagcgc gctgcaccgg aggatgctct cactcataac gcagcgcctc
     2641 cgccggcgcc atgcgcgagg cgcgatacgc cgggtacagc gtggagaaga atcccagtgc
     2701 cagcgacagc agcgagatct tggtcacgtc ggacagcttc agttccgacg gcaggtcgga
     2761 gatgtagtag atgtcgggcg acaggaactg gtgcccggtg gcggcctcga gcagcggcac
     2821 cagcgtctgc acgttctccg ccagcagcac gcccagcacc acgccgaaga aagtgcccat
     2881 cacgccgatc accgagccct gcaccatgaa gatggccatg atgctgcgcg gcgtggcgcc
     2941 gagcgtgcgc aggatggcga tgtcggcctg cttgtcctgc accaccatca ccagcgtgct
     3001 gacgatgttg aacgcggcca cgccgacgat cagcgacagg atgatgaaca tcaccatctt
     3061 ctcggtcgcc accgccttga agaagttggc gtggttgcgc gtccagtcgc tcacgtaata
     3121 gacgccggcg agcgagccgg ccagttcgcg cgccacctgc ggcgcctgga acaggtcgtg
     3181 cagcttgagg cgcacgccgg tcacctgccc cggcatgcgg tacagcgcgg cggcgtcgtc
     3241 gaggtggatc agcaccaggc cgcggtcgaa ctcgtacatg cccacctgga agatgccggc
     3301 cacggtgaag cggcggaagc gcggcagcac gccggccggc gtcacgctgg cctgcgggat
     3361 catcaggtcg atcttgtcgc cgacgatcac gccgagggcc tgcgccagtt cgcggccgat
     3421 gatgatcttg aactcgccgg ccttgaggtc ggtgagcttg ccggccttca tggtcctgcc
     3481 gatgtcggac acggtggctt cgtattccgg cagcacgccg cgcagcatgg tgccggacag
     3541 gtcgctgccc accttcacca tgccctcgcc ctcgatgtac ggcgccaccg cctcgatggc
     3601 cgggttggcg cgcgccttgg ccgccacgtc ctgccagtcg ctcatgccgc cctcgaaggc
     3661 ggagatggtg gcgtgcgaag ccatgcccag gatgcgcgtg cgcagttcgc gctcgaagcc
     3721 gttcatcacc gacagcacgg tgatcagcgc cattacgccc accgcgatgc cgatcatcga
     3781 ggccagcgag atgaagctga tgaaatggtt gcggcgcttg gcgcgcgtgt agcgcaggcc
     3841 ggtgaagagt tcgtagggag ctgccatggg acgcgaaatt agaccacatc gagccctgct
     3901 cggcaggcgc tcttggctgc tgctagccta agtgcagaac atcaaggagc gcgccatgcc
     3961 ggagacggcg ggccaccgtt actggggatt tctcagctac agccacgccg acgaagccgc
     4021 ggcggtgcgg ctgcaccgcg cgctcgaaag ctacgtgctg ccgcgccgcc tgcgccgcgc
     4081 gcatggtttg ccgcggcggc tcacaccgat cttccgcgat gtcgacgagc tggaagccgc
     4141 ctccgggctg aacacgcgcc tgcaggacgc gctggaccag tcgcgctggc tgatcctgct
     4201 gtgctcgccg gccgcggcgg cctcgaaata cgtcaatgcc gaggtcgaat attttctgca
     4261 gcgccatggc gccgcgcgcg tgctgtgcgt gctggcgggc ggcgagccgc cggaatgctt
     4321 tccaccggcg atccgcgcct tgcccgagga gccgctggcg gcagatctgc ggcccgggcg
     4381 cgacgcgcag accgcgcgcc tgaagctggt cgccgcgctg gccggcgtcg gtttcaccga
     4441 gctgcgcaac cgcgaggcgc agcgcc
//
LOCUS       DASZZC010000016         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_129501,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000016 DASZZC010000000
VERSION     DASZZC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129501"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VHE37_00370"
     CDS             complement(<1..644)
                     /locus_tag="VHE37_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34006.1"
                     /translation="MKRLSILPHEYAFNAFLLLVAAREIFQSGSLLIGAAFVLLAVAG
                     GVLVAWTQQCPQPLRWRLRLLWYPCAMGLSFYLLPLAIAALSVPRADALLSGIDGRML
                     GAQAAALLLPYATPAVTDVMTLGYVFFFYYLIFGPAHYFFRDLALLRACFAGMFVTYA
                     LGFTGYLLWPAGGPHLAGQFALALPHGPVSALVLPFIDTASNGVDVFPSIHAAIS"
     gene            complement(719..2128)
                     /gene="glnA"
                     /locus_tag="VHE37_00375"
     CDS             complement(719..2128)
                     /gene="glnA"
                     /locus_tag="VHE37_00375"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HVT34007.1"
                     /translation="MSGKDVLKTIAEKSVKFVDFRFCDTRGKEQHVSVPAHTVDEALF
                     TDGKMFDGSSIAGWKGINESDMILMPDASTAFIDPFFEEPTLVLTCDVIEPSTGQGYS
                     RDPRSLAHRAEAYLKSTGIADTAFFGPENEFFVFDSVRWDAGMKGCFVEIEAENAAWN
                     SGKEFEGGNMGHRPGIKGGYFPVPPVDSLQDIRSAMCETLESVGMVTEVHHHEVATAG
                     QCEIGVKFGTLVKKADEVLKLKYVVQNVAHSFGKTATFMPKPIVGDNGSGMHVHQSLA
                     LKGKNLFTGDGYGGLSETALYYIGGILKHAKTINAFTNPGTNSYKRLVPGFEAPVMLA
                     YSARNRSASIRIPHVQNPKARRIEVRFPDSTANPYFAFAAMMMAGLDGIQNKIHPGEP
                     SDKDLYHLPPEEDKKIPRVCHSLDMALDHLDKNRAFLKQGGVFTDDLIDAYIELKLQE
                     VTRFRMTTHPIEFDMYYSM"
     gene            2344..3261
                     /gene="glyQ"
                     /locus_tag="VHE37_00380"
     CDS             2344..3261
                     /gene="glyQ"
                     /locus_tag="VHE37_00380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HVT34008.1"
                     /translation="MTFQELIFTLQKYWASHGCVIVQPYDMEMGAGTFHTGTFLRSLG
                     PEPWNTAYVQPSRRPTDGRYGENPNRLQHYYQFQVLMKPSPPDIQELYLNSLRVLGFD
                     LLTHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIECRPVSGEITYG
                     LERLAMYIQKKESVYDLVWSDAGGRTVTYGDVFHQNEVEQSHYNFEHADVPALFERFT
                     VAERECQRLLALDQPLPLPAYERVLQGSHAFNLLDARGAISVTERQAYILRVRTLARE
                     VAVAYYNARARIAGFEPLAVAGSNNVIPA"
BASE COUNT          605 a         1085 c         1140 g          575 t
ORIGIN      
        1 gagatggcgg cgtggatcga cgggaacacg tccacgccgt tgctggccgt gtcgatgaac
       61 ggcagcacca gcgccgacac cggcccgtgc ggcagcgcca gcgcgaactg cccggccagg
      121 tgcggcccgc ccgccggcca cagcaggtag ccggtgaagc ccagcgcgta ggtgacgaac
      181 atgccggcga agcaggcgcg cagcagcgcg aggtcgcgga aaaaatagtg cgccgggccg
      241 aagatcaggt agtagaagaa gaacacgtag cccagcgtca tcacgtcggt gaccgcgggc
      301 gtggcgtacg gcagcagcag cgccgcggcc tgcgcgccga gcatgcggcc gtcgatgccc
      361 gacagcagcg cgtccgcgcg cggcaccgac agtgccgcga tggccagcgg cagcaggtag
      421 aacgacaggc ccatcgcgca cgggtaccac agcaggcgca ggcgccagcg cagcggctgc
      481 gggcactgct gcgtccaggc gaccagcaca ccgccggcaa cggccagcag cacgaaggcg
      541 gcgccgatca gcagcgagcc ggactggaaa atttcgcgcg cggcgacaag caacaggaac
      601 gcgttgaacg cgtactcgtg cggcaggatg gaaagacgtt tcatgaagcc gcgcactttg
      661 cgcgaacgcg gccccaaaaa gaaagccccc ttgcgggggc ttccggcact gcgctcgatt
      721 acatcgagta gtacatgtcg aactcgatcg gatgcgtggt catgcggaag cgcgtgacct
      781 cctgcagctt cagttcgatg taggcgtcga tcaggtcgtc ggtgaacacg ccgccctgct
      841 tgaggaacgc gcggttcttg tccaggtgat ccagcgccat gtccaggctg tggcacacgc
      901 gcgggatctt cttgtcctct tccggcggca ggtggtacag gtccttgtcg ctcggctcgc
      961 ccgggtggat cttgttctgg atgccgtcca ggcccgccat catcatcgcc gcgaaggcga
     1021 agtacgggtt ggcggtggaa tccgggaagc gcacttcgat gcggcgcgcc ttcgggttct
     1081 gcacgtgcgg gatgcggatc gacgccgagc ggttccttgc gctgtaggcc agcatgaccg
     1141 gcgcttcgaa gcccggcacc aggcgcttgt agctgttggt gcccgggttg gtgaaggcgt
     1201 tgatggtctt ggcgtgcttg aggatgccgc cgatgtagta cagcgcggtc tccgacaggc
     1261 cgccgtagcc gtcaccggtg aacaggttct tgcccttgag cgccagcgac tggtgcacgt
     1321 gcatgccgct gccgttgtcg ccgacgatcg gcttgggcat gaaggtggcg gtcttgccga
     1381 acgagtgggc cacgttctgc accacgtact tcagcttgag cacttcgtcg gccttcttca
     1441 ccagcgtgcc gaacttgacg ccgatttcgc actggccggc ggtggccact tcatggtggt
     1501 gcacctcggt gaccatgccg acggattcca gcgtctcgca catggcggag cggatgtcct
     1561 gcagcgagtc gaccggcggc accgggaagt aaccgccctt gatgcccgga cggtgaccca
     1621 tgttgccgcc ttcgaattcc ttgcccgagt tccaggccgc gttctcggcc tcgatctcga
     1681 cgaagcagcc cttcatgccg gcgtcccagc gcacggagtc gaacacgaag aattcattct
     1741 ccgggccgaa gaaggcggtg tcggcgatgc cggtgctctt caggtaggcc tcggcgcggt
     1801 gcgccagcga gcgcgggtcg cgcgagtagc cctggccggt cgaaggctcg atgacgtcgc
     1861 aggtcagcac cagcgtcggc tcttcgaaga acgggtcgat gaaggcggtg gacgcgtcgg
     1921 gcatgaggat catgtcggac tcgttgatgc ccttccagcc ggcgatcgag ctgccgtcga
     1981 acatcttgcc gtcggtgaac agcgcttcat ccaccgtgtg agcgggcacc gacacgtgct
     2041 gctccttgcc gcgcgtgtcg cagaaacgga agtcgacaaa cttcacgctc ttttccgcaa
     2101 ttgttttcag tacgtctttg cccgacatga ggccatctcc caggatgctg ttgattgaaa
     2161 acgcccgggg tcaccgagcg tgcgccaaaa tgaagcaggt tctatgccac cggttgccgg
     2221 tgagcgggcg gcatcatagc ccaaagccct gtgcggaaag cgcgccacaa atcagcgcgg
     2281 ggaacggggc ggcgggagcg cgttgctata ctcgccggcc gcgcaaaaca ccgaagaatt
     2341 cccgtgacgt tccaagagct gatcttcacg ctccagaagt actgggccag ccacggctgc
     2401 gtcatcgtgc agccctacga catggagatg ggcgccggca ccttccacac gggcaccttc
     2461 ctgcgctcgc tcgggccgga gccgtggaac acggcctacg tgcagccgtc gcgccgtccc
     2521 accgacgggc gctacggcga aaaccccaac cggctgcagc actactacca gttccaggtg
     2581 ctgatgaagc ccagcccgcc ggacatccag gaactgtatc taaattcgct gcgcgtgctg
     2641 ggctttgacc tgctgacgca cgacatccgc tttgtcgaag acaactggga gtcgcccacg
     2701 ctcggcgcct ggggcctggg ctgggaggtg tggcttaacg gcatggaggt cacgcagttc
     2761 acctacttcc agcaggtggg cggcatcgag tgccggccgg tgtcgggcga gatcacctac
     2821 ggactggaac gcctggccat gtacatccag aagaaggaga gcgtgtacga cctggtctgg
     2881 tccgatgccg gcggccgcac ggtgacttac ggcgacgtgt tccaccagaa cgaggtggag
     2941 cagagccact acaacttcga gcatgccgac gtgccggcgc tgttcgagcg cttcaccgtg
     3001 gccgagcgcg aatgccagcg cctgctcgcg ctggaccagc cgttgccgct gcccgcctac
     3061 gagcgcgtgc tgcagggctc acacgcgttc aacctgcttg acgcgcgcgg cgcgatcagt
     3121 gtgaccgagc ggcaggccta catcctgcgc gtgcgcacgc tggcgcgtga agtggcggtg
     3181 gcgtactaca acgcccgcgc gcgcattgcc ggttttgaac cgctcgccgt cgccggttcc
     3241 aacaacgtca ttcccgcgta ggcgggaatc caggtttatg tctcagcaac tacttattga
     3301 aatcgggacc gaagacctgc ccgcgcgcta tgtgatgccg ctggcgaatg cgctggctgc
     3361 cggcgttgct gaaggcctgc gcaagcgcgg cgtcgcgcag ggccc
//
LOCUS       DASZZC010000017        11702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_578680,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000017 DASZZC010000000
VERSION     DASZZC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11702
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578680"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /gene="carB"
                     /locus_tag="VHE37_00385"
     CDS             complement(<1..1657)
                     /gene="carB"
                     /locus_tag="VHE37_00385"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HVT34009.1"
                     /translation="MPNRTDIQSILIIGAGPIVIGQACEFDYSGAQACKALRQEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIRWQTVEKIIEKERPQALLPTMGGQTALNCALDL
                     EKHGVLAKYGVQMIGANATAIDVAEDRQKFRDAMTEIGLGSARSGVAHTMEEARTIQA
                     TIGYPVIIRPSFTLGGTGGGVAYNVEEFEEIVMRGLDLSPTTEVLIEESLLGWKEFEM
                     EVVRDRKDNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQLLRNASIAVLRRIG
                     VDTGGSNVQFAINPADGRMIIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LQNDITGGKTPASFEPSIDYVVTKIPRFTFEKFPQADSRLTTQMKSVGEVMAIGRNFQ
                     ESLHKAMRGLEVGSDGFEPQVNDASEISIARIQAELSTARAKRLWFVGDAFRAGMSVA
                     EVHKLSKIDPWFLEQIEELIDVELEIGAHKLGTLKADSLRRYKRLGFSDRRIAKLLGV
                     KEESVRARRHKLGVRPVYKRVDTCAAEFPTSTAYLYSSYDEECE"
     gene            complement(1670..2311)
                     /gene="leuE"
                     /locus_tag="VHE37_00390"
     CDS             complement(1670..2311)
                     /gene="leuE"
                     /locus_tag="VHE37_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HVT34010.1"
                     /translation="MFGIVDLPNYLAIVVVTILLPGPNSMFVLSVAARQGVADGYKAA
                     LGVFVSDSILMVVSVTGVASILHAYPQLFTALKLAGAGYLAWIGVSLVRSGLNAARAP
                     VEAELPAPVRVEDPFRRVVLIGLLNPKAIFFFMAFFIQFVDRSYAHPALSFVLLGAML
                     QAISMLYLSMLIFAGARLARAFRERRRLAAGATGGAGLMFLGFSAKLASMAAQ"
     gene            complement(2315..3466)
                     /gene="carA"
                     /locus_tag="VHE37_00395"
     CDS             complement(2315..3466)
                     /gene="carA"
                     /locus_tag="VHE37_00395"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HVT34011.1"
                     /translation="MTPPKTAPGKAPGRAILALADGSIFEGVSIGADGRTVGEVVFNT
                     AMTGYQEILTDPSYREQIVTLTYPHIGNYGSNTGDAESDRVHIAGLVLREVPRPHSSW
                     RSTQDFREYLRANKVVAIAGIDTRRLTRILRDKGAQNGCIMAGKVSEAAALKAARAFP
                     GLKGMDLAKVVSRTKPESWTRGSWELASNREKIIEGGKYHVVAYDFGVKNNILRMLVD
                     RGCRVTVVPAQTPAADVFRLKPDGVMLTNGPGDPEPCDYAIRAAREFMTKKLPLFGIC
                     LGHQILGLAAGARTLKMKFGHHGANHPVQDLESGRVLITSQNHGFAVDEQTLPANVKV
                     THRSLFDGSNQGLRVTDAPAFSFQGHPEASPGPHDVGYLFDRFVESMKG"
     gene            3686..5077
                     /locus_tag="VHE37_00400"
     CDS             3686..5077
                     /locus_tag="VHE37_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVT34012.1"
                     /translation="MTWGKCRRAVGSLLMALALCAGSATVHAQDAAPAASAEPSAPAA
                     APAPAPTKPFLVSFDKINPGDTAWMLASTALVLMMTIPGLALFYAGMVRKKNVLSTAL
                     QSFFICCLVTVLWVICGYSMAFTPGPTPFLGGFDRVLLNGMLYLHDAGKVTVSHVATT
                     IPESVYVMFQMTFAIITPALITGAFAERIRFGAMALFMTLWALLVYAPVAHWVWEPSG
                     WLASMGAQDFAGGTVVHINAGAAGLACAFVVGDRRGFGREAFIPHNLGLTLIGASLLW
                     VGWFGFNAGSAVASDGRAGMAMLVTQLATAVASLTWMLAEVLMRRRATLLGACSGAVA
                     GLVAITPASGYVNVIGAFAIGIAAGCACYWGATGLKKLLGTDDSLDVFGIHGIGGLVG
                     ALLTGVFADKAICGADGNIGVQALAAFGTLIYSGMMSLIILFMIDIGIGLRVNTEDEQ
                     LGLDLSQHGEQIG"
     gene            5107..5415
                     /locus_tag="VHE37_00405"
     CDS             5107..5415
                     /locus_tag="VHE37_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34013.1"
                     /translation="MADNNAELQSVVGHLYVRLRRDCARVIDALWAARNAEYAAEILK
                     VARTVRDPEMDKLTARYEELMFGTRRPAAHAPSPAASTPSDAELDGLSNTGRYRQSLR
                     "
     gene            complement(5536..6441)
                     /locus_tag="VHE37_00410"
     CDS             complement(5536..6441)
                     /locus_tag="VHE37_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HVT34014.1"
                     /translation="MELLCHSFHAMGGPCEARVYGRDATHARAAAQAAQDEVLRLERK
                     YTRYRDDSLTSAINRSAGDARGIEVDDETAALLDYADTAWQQSEGAFDITSGVLRRAW
                     NFRSGKVPAQAQIDAILPLVGWPQVRWSRPRLVLPLAGMELDFGGYVKEYAADRAAQC
                     ARDAGCAHGFVELGGDIALIGPHPGGAPWQIGVRDPRARERALVSVALAQGALASSGD
                     YERCMEIGGVRYAHILDPRSGWPVRGLTAVSVIAPQCLVAGTATTIAMLKGEDGLHWL
                     EELGLRWMAVDAAGRLHGDIGAAAR"
     gene            complement(6458..7675)
                     /locus_tag="VHE37_00415"
     CDS             complement(6458..7675)
                     /locus_tag="VHE37_00415"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HVT34015.1"
                     /translation="MSQKKSNALLALTASALAIPGLVRPAAAAEDNMQVDYRYSAYRE
                     GDLDADRSASNSKVGRYHVNTHQIHGNWELAPGSDISADVVVETMSGASPWYITPAAG
                     TNKPVQVMSGASIEDSRQALTLGRKQRFGDGTLDLHGSYSNERDYRSGDGGLGWSYDF
                     GENLNTVSIGASYTVNRLQPTDGGVVPGRIVSADSHAAGATLAYTRVLGADSQAQLGV
                     SYTQEAGFLSDPYKLAYLQNMPGNPGTPGTVPDERPDGRREWAAQARLRQWLAPLNAA
                     LALDYRYYRDDWQISSHTVDVAYRQQLPGGWEIAPGVRWYSQSQSYFYAPYYAGVRSD
                     NFASSDYRLSPYGALSWRLEASSGKLLGPVALRLRYENYRSKGSYAVGKVVEENPGLV
                     DFSVISASIGAIF"
     gene            complement(7683..7895)
                     /locus_tag="VHE37_00420"
     CDS             complement(7683..7895)
                     /locus_tag="VHE37_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HVT34016.1"
                     /translation="MSMRNLLLMCACALLCACATPKAWQRGHLAQPAMAWEPDATDAA
                     IREHTYVSKEAAIGGVNGAGGGCGCR"
     gene            complement(7892..8383)
                     /locus_tag="VHE37_00425"
     CDS             complement(7892..8383)
                     /locus_tag="VHE37_00425"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVT34017.1"
                     /translation="MRKLARGLLLALLPLCAQAAQQGDVAPVFSAPALEGGALVNLAD
                     YHGRVVLVDFWASWCAPCKQALEHYQQLRRDWHARGFEVVAVNVDENSGDGRRLARPL
                     ALEFPLVEDAKGRVAELYAVATMPSAYLIDRRGVVRWVLQGYRGDEGAALDDRVRKLL
                     EEK"
     gene            complement(8380..11196)
                     /locus_tag="VHE37_00430"
     CDS             complement(8380..11196)
                     /locus_tag="VHE37_00430"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34018.1"
                     /translation="MPMKIHAAMLLAAAMLGACSGTYSGTPQASAGGSGTPTGGGAGS
                     TFASDKDFFTARVQPNLGFCRTCHVPQGVADTDTGKLMMLSPDPAQDYANLQASWIAL
                     AKGVDSNLILQNPSGAHAHSGGAPWPSTSASYAAMKLLLSCWDQPSGCAALLAAATSG
                     SDPAPLPLLENPGKHYFVSQICDGQPDSTPIDWARDPRRFMSGANIDNDNYAVYFNDP
                     FEICHNDTLIQNQASENALLTAASKTPIHTAKPYAATCGEWRARVKEGHDWIAYSPTD
                     TPREGGQGNGAFGVTDYSAQAWNKLWVVWGMSARPDNFDDQVIERYGHSPTPARPGSA
                     SGSYDHDYPNPYPVIDAARGIDETTKLTKTFGGTGHLPLGFAQGRDANGVYNGKLSLN
                     CFSCHAGQVGSGEIASRDGINDSESYGGNPAGSFMGLPNTNTELGVLIIDLIHAQQPV
                     NLHIPPVGYLPLVNTTRGTNAADTEIEAIVAIRDFDTLNFDHAFVDPVHASFGDQDPP
                     AWWWLHNKSRYLWFGGHSTDSSRGNMYFGSVNGLSGDDVKRNEGIFESVHDWSLTVEA
                     PDYPQGYCSGTDGSAVAGDKPGCINRALAEQGAILFHEKNLWADGQNADIPKPHGNGA
                     CASCHGAYAPRYASDPRFLPDPKMIGETGYTVPIEIINTDPAQATGWSAEIRPFVSTF
                     WWSYPDATPDYRFPESKDPLTEFLDDYSGYVDGLSNGPAMADHLDRYLDGMGLLQPLG
                     DVLQTVLGATLPNFPPVPLGATVGRVKGACGFEEKTIGYVTPPLHGVWASGPYFHNGS
                     VPTVWDVLKPSDRPAVWRRQRTSTSVEVNAFEHRLSGYDFDHLGWKYDTLTCGDGGQG
                     IPYYTCEPGTPLPPEIDWIKTTLDGGLIWPTWVVPPPFGDAGLADRMIYNTNLYSKKN
                     SGHQWTQVLTDNERKALLEYLKTL"
     gene            11312..>11702
                     /locus_tag="VHE37_00435"
     CDS             11312..>11702
                     /locus_tag="VHE37_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HVT34019.1"
                     /translation="MKPADPNKLWIPAVYGLLIIDLVEQRGVSRTKLLDGIGIPDAAW
                     REVDGRISLIQAGAMLYKGVKLTGDGALGYEIGLSSNLTTHGFVGYGLLSHATVREAV
                     LFGAGVLPLRLPNLSLRLFEDGAQGVVD"
BASE COUNT         1860 a         3932 c         3825 g         2085 t
ORIGIN      
        1 cctcgcattc ctcgtcgtag ctcgaataca ggtacgcggt cgacgtcggg aactccgccg
       61 cgcaggtatc cacgcgcttg tacaccgggc gcacgcccag cttgtgccgg cgcgcgcgca
      121 ctgactcctc cttgacgccg agcagtttcg caatgcggcg gtcggagaaa cccaggcgct
      181 tgtagcggcg caggctgtcg gccttgagcg tgccgagctt gtgcgcgccg atttccagtt
      241 ccacgtcgat cagctcctcg atctgctcga ggaaccacgg atcgatcttc gacagcttgt
      301 gcacctcggc cacgctcatg ccggcgcgga aggcatcgcc gacgaaccac aaccgcttgg
      361 cgcgcgccgt ggacagctcg gcctggatgc gcgcgatgga aatctccgat gcgtcgttca
      421 cctgcggctc gaagccgtcg gaacccactt ccagcccgcg catggccttg tgcagcgatt
      481 cctggaagtt tcggccgatg gccatgacct cgcccaccga cttcatctgc gtggtcaggc
      541 gcgagtcggc ctgcgggaat ttctcgaagg tgaatcgcgg gatcttggtc accacgtaat
      601 cgatcgacgg ctcgaacgag gccggcgtct tgccgccggt gatgtcgttc tgcagctcat
      661 ccagcgtgta gccgaccgcg agcttggccg cgattttcgc aatcgggaat cccgtggcct
      721 tcgacgccag cgccgacgag cgcgacacgc gcggattcat ctcgatgatg atcatgcgcc
      781 cgtccgccgg gttgatggcg aactgcacat tgctgccgcc ggtatcgacg ccgatgcggc
      841 gcagcacggc gatgctggcg ttgcgcagca gctggtattc cttgtcggtc agtgtctgcg
      901 ccggcgccac cgtgatcgag tcgccggtgt gcacgcccat cggatcaaaa ttctcgatgg
      961 agcagacgat gatgcagttg tccttgcggt cgcgcaccac ttccatctcg aactctttcc
     1021 agccaagcag cgattcctcg atcagcactt ccgtggtcgg cgacaggtcg aggccgcgca
     1081 tcacgatctc ctcgaactcc tccacgttgt aggccacgcc gccgccggtg ccgccgagcg
     1141 tgaacgaggg gcggatgatc accggatagc cgatggtcgc ctggatggtg cgcgcctctt
     1201 ccatggtgtg cgccacgccc gagcgcgccg agcccaggcc tatttcggtc atggcatcgc
     1261 ggaatttctg gcggtcttcg gccacgtcga tggccgtggc attggcgccg atcatctgca
     1321 ccccgtattt cgccagcacg ccgtgctttt caagatcgag cgcgcagttc agcgcagtct
     1381 gcccgcccat ggtcggcagc aatgcctgcg gccgctcctt ctcgatgatc ttctcgacgg
     1441 tctgccagcg gatcggctcg atgtacgtgg cgtcggccat ctccgggtcg gtcatgatgg
     1501 tcgccgggtt gctgttgacc aggatgacgc ggtaaccctc ctggcgcagc gccttgcatg
     1561 cctgcgcgcc cgagtagtcg aactcgcagg cctggccgat gacgatcggg ccggcgccga
     1621 tgatcaggat gctttggatg tcggtacggt ttggcatcgt aagaactcgc tattgcgccg
     1681 ccatcgaggc gagcttggcg gaaaacccga ggaacatgag gccggcgccg ccggtggcgc
     1741 ccgcggccag gcggcgacgc tcgcggaacg cccgcgccag cctggcgccg gcgaagatca
     1801 gcattgacag gtacagcatg ctgatcgcct gtagcatcgc gcccagcagc acgaacgaca
     1861 gcgccgggtg cgcatagctg cggtcgacga actggatgaa gaaggccatg aaaaagaaga
     1921 tcgccttcgg attgagcagg ccgatcagca ccacgcggcg gaacggatcc tccacgcgga
     1981 ccggcgccgg gagttccgcc tcaaccggcg cgcgcgcggc gttcagcccg ctgcgcacca
     2041 ggctcacgcc gatccaggcc aggtagccgg cgcccgccag cttgagcgcg gtgaacaact
     2101 gcggataggc atgcaggatc gaggcaacgc cggtcaccga taccaccatc aggatgctgt
     2161 cgctgacgaa cacgcccaac gccgccttgt acccgtccgc tacgccctgg cgcgccgcca
     2221 cgctaagcac gaacatcgag ttcggaccgg gcagcaggat ggtcaccacc acgatcgcca
     2281 ggtagttggg caggtcgacg atgccgaaca ttgctcagcc tttcatcgac tcgacgaagc
     2341 ggtcgaacag gtagccgaca tcgtgcggcc ccgggcttgc ttccgggtgg ccctggaacg
     2401 agaacgcggg cgcgtcggtg acgcgcaggc cctggttgct gccgtcgaac agcgagcggt
     2461 gcgtgacctt cacgttggcc ggcagcgtct gctcatccac cgcgaagccg tggttctggc
     2521 tggtgatgag cacgcggccg gattcgagat cctgcaccgg gtggttggcc ccgtgatggc
     2581 cgaacttcat cttcagcgtc ctggcgccgg cggccagtcc gagaatctgg tggccgagac
     2641 agatgccgaa cagcggcagt ttcttcgtca tgaattcgcg cgcggccctg atcgcgtagt
     2701 cgcagggctc aggatcgccc gggccgttgg tcagcatcac gccgtcgggc ttgagcctga
     2761 acacgtctgc tgccggcgtc tgcgccggca ccacggtaac gcggcagccg cgatccacca
     2821 gcatgcgcag gatgttgttc ttcacgccga agtcgtaggc gacgacgtga tacttgccgc
     2881 cttcgatgat cttctcgcgg ttgctcgcca gttcccagga tccgcgcgtc cagctctccg
     2941 gtttcgtacg gctgaccacc ttggccaggt ccatgccctt gagtcccggg aaggcgcgcg
     3001 cggctttcag agccgccgct tcgctcacct tgcccgccat gatgcagccg ttctgtgcac
     3061 ccttgtcgcg caggatgcgc gtcaaacggc gcgtgtcgat acccgcgatg gccaccacct
     3121 tgttcgcgcg caggtactcg cggaaatcct gcgtcgagcg ccaggaggaa tgcggccgcg
     3181 gcacctcgcg cagcaccagg ccggcaatgt ggacgcggtc ggactcggca tccccggtgt
     3241 tgcttccgta gttgccgatg tgcgggtaag tcagcgtgac gatctgctcg cggtacgagg
     3301 gatcggtgag gatttcctgg tagccggtca tggcggtgtt gaacaccacc tcgccgacgg
     3361 tgcggccatc ggcaccgatg gacacgcctt cgaaaatgga gccatcagcg agcgcaagaa
     3421 tggcgcggcc gggtgctttg cctggggccg ttttgggagg agtcatcggg tagatctaat
     3481 ccggttgcgg gcgcgaaaaa cggcgccgcg agacgccgtt ggattaggcg gacattgtac
     3541 tggaacgccc cggtccataa cagcgcacag cgaaatattt ttgcgcgatc tgcgtgcacg
     3601 aacagcgact tcgccgacgt gccgatcagt tggcatccaa cgtgcttacc tctcagtcag
     3661 tcgaccaaca agggagcatg cgacgatgac ctggggcaaa tgccgtcgcg cggtgggcag
     3721 cttgctgatg gcgctggcgc tgtgcgcggg ttcagccacc gtccatgcgc aggacgcggc
     3781 accggctgca tcggcggagc cttccgcgcc ggccgccgcg cccgcgccgg cgccaaccaa
     3841 gcccttcctg gtcagcttcg acaagatcaa tcccggcgac accgcctgga tgctcgcgtc
     3901 caccgcgctg gtgctgatga tgaccatccc cgggctggcc ctgttctacg ccggcatggt
     3961 gcgcaagaag aacgtgctgt cgaccgcgct gcagagtttc ttcatctgct gcctggtcac
     4021 cgtgctgtgg gtgatctgcg gctacagcat ggcgttcacg cccggcccga caccctttct
     4081 gggcggcttt gaccgtgtgc tgctcaacgg catgctctac ctgcacgacg ccggcaaggt
     4141 caccgtgagc cacgtggcca ccaccatccc ggaatcggtc tacgtgatgt tccagatgac
     4201 cttcgccatc atcacgccgg cgctgatcac cggtgccttc gccgagcgca tccgcttcgg
     4261 cgccatggcg ctgttcatga cgctgtgggc gctgctggtc tacgcgccgg tggcgcactg
     4321 ggtgtgggaa cccagcggct ggctggcctc gatgggcgcg caggatttcg ccggcggcac
     4381 cgtggtgcac atcaacgccg gcgccgccgg cctcgcctgc gccttcgtgg tcggcgaccg
     4441 gcgcggcttc gggcgcgagg cgttcattcc gcacaatctc ggcctcacgc tgatcggcgc
     4501 ctcgctgctg tgggtcggct ggttcggctt caacgccggc tcggcggtgg cctccgacgg
     4561 tcgcgccggc atggccatgc tggtcacgca gctcgccacc gccgtcgcct cgctcacctg
     4621 gatgctggcc gaggtgctca tgcgcaggcg cgcgacgctg ctcggcgcct gctccggcgc
     4681 cgtggccggg ctggtggcga tcacgccggc ttccggctac gtcaacgtga tcggcgcctt
     4741 cgccatcggc atcgccgccg gctgcgcctg ctactggggc gccaccgggc tgaagaaact
     4801 gctgggcacc gacgactcgc tcgacgtgtt cggcatccac ggcatcggcg gcctggtcgg
     4861 cgccctgctc accggcgtgt tcgccgacaa ggccatctgc ggcgccgacg gcaacatcgg
     4921 cgtgcaggcg ctggccgcgt tcggcacgct gatctacagc ggcatgatgt cgctgatcat
     4981 cctgttcatg atcgacatcg gcatcggcct gcgcgtgaac accgaggacg aacagctggg
     5041 tctcgatctg tcgcagcacg gagaacagat cggctgatga tttccttgag ctgaaggcgg
     5101 atgggcatgg cagacaacaa tgcggaactg cagtcggtgg tgggtcatct ctacgtgcgc
     5161 ctgcggcgcg actgcgcgcg cgtgattgac gcgctgtggg ccgcgcgcaa cgccgagtac
     5221 gcggcggaaa tcctcaaggt ggcgcgcacg gtcagggacc cggagatgga caagctcacc
     5281 gcgcgctacg aggaactgat gttcggcacg cggcgcccgg ctgcgcacgc accctcgccc
     5341 gccgccagca cgccgagcga cgccgaactg gatggcctgt cgaacaccgg acgctaccgc
     5401 cagtcgctgc gctgattttt cgagccgcaa agacctgaac cgcactgctg gtttgcctgt
     5461 tattatttat attaatattt taactttaaa taataataaa taacatcaaa aataccgatg
     5521 cggtggcggc agatttcagc gcgcagccgc accgatgtcg ccatgcagcc ggcccgcggc
     5581 atccaccgcc atccagcgca ggccgagttc ctccagccag tgcagcccgt cctcgccctt
     5641 gagcatggcg atggtcgtcg ccgtgccggc gaccaggcat tgcggcgcga tcacgctcac
     5701 cgctgtgagc ccgcgcaccg gccagccgct gcgcggatcg aggatatgcg cgtagcgcac
     5761 gccgccgatc tccatgcagc gctcgtagtc gccgctggat gccagcgcgc cttgcgcgag
     5821 cgcaacgctc accaacgccc gctcgcgtgc gcgcggatcg cgcaccccga tctgccacgg
     5881 cgcgccaccg ggatgcggac cgatcagcgc gatgtcgccg ccgagttcga cgaagccgtg
     5941 cgcgcagccg gcgtcgcgcg cgcattgcgc cgcacggtcg gcggcgtatt ccttcacgta
     6001 gccgccgaaa tccagttcca tgcccgcaag cggaagcacc aggcgcggcc gcgaccagcg
     6061 cacctgcggc cagcccacca gcggcaggat cgcatcgatc tgcgcctgcg ccggaacctt
     6121 tcccgacctg aaattccacg cgcgccgcaa cacaccggag gtgatgtcga aggcgccttc
     6181 gctctgctgc cacgcggtgt ccgcatagtc gagcaacgcg gcggtttcgt cgtccacttc
     6241 aattccacgc gcgtcgcccg cgctgcgatt gatcgcggag gtcaggctgt cgtcgcgata
     6301 gcgcgtgtac ttgcgctcca gccgcagcac ttcatcctgc gcggcctgcg ccgctgcacg
     6361 tgcatgagtt gcatcgcgcc cgtagacgcg tgcttcgcaa ggaccaccca ttgcatggaa
     6421 cgagtggcac agcagttcca tcggaatacg ccgcggttca gaagatggcg ccgatgctgg
     6481 ccgagatcac gctgaagtcg accagccccg gattttcttc caccaccttg cctaccgcgt
     6541 agctgccctt gctgcggtag ttctcgtagc gcaggcgcag cgccaccggc cccagcaact
     6601 tgccgctgct ggcttccagc cgccacgaca gcgcaccgta cggcgacagg cgatagtcac
     6661 tcgaagcgaa gttgtcgctg cgcacgccgg cgtaatacgg cgcatagaaa tacgactggc
     6721 tctggctgta ccagcgcacg ccgggcgcga tctcccagcc gccgggcagc tgctggcgat
     6781 acgccacgtc gaccgtgtgc gaactgatct gccagtcgtc gcggtagtag cggtagtcca
     6841 gcgccagcgc cgcgttgagc ggcgccagcc attgccgcag gcgcgcctgc gcggcccatt
     6901 cgcggcggcc gtcggggcgc tcgtcgggca ccgtgcccgg ggtcccggga ttacccggca
     6961 tgttctgaag gtaggccagc ttgtaaggat cggacaggaa acccgcctcc tgcgtgtagc
     7021 tgacgccgag ttgcgcctgt gagtcggcac cgagcacgcg cgtgtaggca agcgtggcgc
     7081 cggccgcgtg gctgtcggcg ctcacgatgc gcccaggcac tacaccccca tcagtgggct
     7141 gcagccggtt gacggtgtag ctggcgccga tgctgacggt gttgaggttc tcgccgaagt
     7201 cgtagctcca gccaaggccg ccatcgccgg agcggtagtc gcgctcgttg gaatagctgc
     7261 cgtggagatc cagcgtgccg tcgccgaagc gctgcttgcg gccgagcgtg agtgcctgcc
     7321 gcgagtcctc gatgctggcg ccgctcatca cctgaactgg cttgttcgtg cccgcagccg
     7381 gcgtgatgta ccagggtgat gcgccggaca tggtttccac caccacgtcg gcgctgatgt
     7441 ccgatcccgg cgccaattcc caattgccgt ggatctggtg cgtgttgacg tggtaacggc
     7501 ccaccttgct gttgctggcg ctgcggtcgg catcgagatc gccctcacgg taggccgagt
     7561 agcggtaatc cacctgcatg ttgtcctcgg ccgccgcggc cggccgcacc agcccgggga
     7621 tggccagcgc cgatgcggtc aacgccagca gtgcatttga tttcttctgg ctcatgcgcg
     7681 tgtcaccggc agccgcagcc gccgccggcg ccgttgacgc cgccgatggc tgcttccttg
     7741 ctcacatagg tgtgctcgcg gatggcggcg tcggtggcat ccggctccca ggccatggcc
     7801 ggctgcgcca ggtggccgcg ctgccaggcc ttgggcgtcg cgcacgcgca cagcagcgcg
     7861 caggcgcaca tcagcagcag gttccgcatg ctcatttctc ctccagcagt tttcgtacgc
     7921 ggtcatccag cgccgcgcct tcgtcgcccc ggtaaccctg cagcacccag cgcaccacgc
     7981 cgcggcgatc gatcagatac gccgacggca tcgtcgccac cgcgtacagt tccgccacgc
     8041 ggcccttcgc atcttccacc agcgggaatt ccagcgccag cgggcgcgcc agcctgcggc
     8101 cgtcgccgga attttcatcg acgttcaccg ccaccacttc gaagccacgc gcgtgccagt
     8161 cgcggcgcag ctgctggtaa tgctccaagg cctgcttgca cggcgcgcac cacgaggccc
     8221 aaaaatccac cagcaccacg cggccgtgat agtcggccag gttcaccagc gcgccacctt
     8281 ccagcgccgg cgcggagaac accggcgcca cgtcaccctg ttgcgcagcc tgcgcgcaca
     8341 gcggcagcag tgccagcagc aggccgcgcg cgagtttcct cacaaggtct tcaggtattc
     8401 gagcaacgcc ttgcgctcgt tgtcggtcag cacctgggtc cattggtggc cgctgttctt
     8461 cttcgagtac aggttggtgt tgtagatcat acggtcagcc agcccggcat cgccaaacgg
     8521 cggcggcacc acccaggtgg gccatatcag cccgccatcg agcgtggtct tgatccagtc
     8581 gatctccggc ggcagcggcg tgcccggctc gcaggtgtag tacgggatgc cctgcccgcc
     8641 atcgccgcag gtcagcgtgt cgtatttcca gcccaggtgg tcgaagtcat agcccgacag
     8701 gcgatgctcg aatgcgttga cttccacgct ggtggaggtg cgctggcggc gccacaccgc
     8761 cgggcgatcg gacggcttga gcacatccca caccgtgggc accgagccgt tgtggaaata
     8821 aggcccgctg gcccacacgc cgtgcagcgg cggcgtgaca tagccgatgg tcttctcctc
     8881 gaagccgcag gcgcctttca cgcggccgac ggtggcgccc agcggcaccg gcggaaaatt
     8941 cggcagcgtg gcgcccagca ccgtctgcaa cacgtcaccc agcggctgca gcagacccat
     9001 gccgtcgaga tagcggtcca ggtgatcagc catggccggg ccgttcgaca gtccatcgac
     9061 atagccgctg tagtcgtcga ggaattccgt cagcgggtcc ttgctctcgg ggaacctgta
     9121 atccggcgtc gcgtccgggt acgaccacca gaaggtgctg acgaagggtc ggatctccgc
     9181 cgaccagccg gtggcctgcg ccggatcggt gttgatgatc tcgatcggca cggtgtagcc
     9241 ggtctcgccg atcatcttcg gatcgggcag gaaacgcggg tcgctggcgt agcgcggcgc
     9301 gtacgcgccg tggcagctgg cgcaggcgcc gttgccgtgc ggcttgggaa tgtccgcatt
     9361 ctggccgtcg gcccacaggt tcttctcgtg gaacaggatg gcgccctgct cggccagcgc
     9421 gcggttgatg cagcccggct tgtcgccggc aacggccgaa ccgtcggtac cgctgcagta
     9481 gccctgcggg tagtcgggtg cctcgacggt cagcgaccag tcgtgcaccg attcgaaaat
     9541 gccctcgttg cgcttgacgt cgtcaccgga caggccgttg acgctgccga agtacatgtt
     9601 gccgcgcgag gagtcggtcg aatgcccgcc gaaccagaga tagcgcgact tgttgtgcag
     9661 ccaccaccat gccggcgggt cctggtcgcc gaagctcgca tgcaccggat cgacgaaggc
     9721 atggtcgaag ttgagcgtgt cgaaatcgcg gatggcgacg atcgcctcga tctcggtatc
     9781 cgccgcgttg gtgccgcgcg tggtgttgac cagcggcagg tagcccaccg gggggatatg
     9841 caggttcacc ggctgctgcg cgtggatcag gtcgatgatc agcacgccca gctcggtgtt
     9901 ggtgttaggc aggcccatga agctgccagc gggattgccg ccgtagctct cgctgtcatt
     9961 gatgccgtcg cggctggcga tttcacccga gcccacctgg ccggcgtgac aggaaaagca
    10021 attgaggctc agcttgccgt tgtacacacc attcgcatcg cggccctgcg caaaacccag
    10081 cggcaggtgg ccggtaccac cgaacgtttt ggtcagcttc gtggtctcgt cgatgccgcg
    10141 tgcagcatcg atgactgggt aaggattggg gtagtcgtga tcgtaggagc cgctggcgct
    10201 gcccggccgc gccggcgtgg gcgaatgtcc gtagcgctcg atcacctggt catcaaaatt
    10261 gtccggccgt gcgctcatgc cccagaccac ccacagcttg ttccaggcct gcgcggagta
    10321 atcggtgacg ccgaaagcgc cgttgccctg cccgccttcg cgcggcgtat cggtcggcga
    10381 gtaggcgatc cagtcgtggc cctccttcac gcgcgcgcgc cactcgccac aggtggcggc
    10441 gtacggcttc gccgtgtgga tcggcgtctt gctcgcggcg gtcagcagcg cgttctccga
    10501 cgcctggttc tggatcagcg tgtcgttgtg gcagatctcg aacgggtcgt tgaagtaaac
    10561 cgcatagttg tcgttgtcga tattggcgcc ggacatgaag cggcgtggat cgcgcgccca
    10621 gtcgatcggc gtggaatccg gctggccatc gcagatctgg ctgacgaagt aatgcttgcc
    10681 cggattttcc agcagcggca gtggcgcggg atcgctgccg ctggtggctg cggccagcag
    10741 cgccgcgcaa cccgacggct ggtcccagca cgacagcagc agcttcatcg cggcgtagct
    10801 ggcggaggtt gacggccatg gcgcgccacc cgagtgcgcg tgcgcgcccg aagggttctg
    10861 caggatcagg ttgctgtcga cgcccttggc cagcgcgatc cacgaagcct gcaggttggc
    10921 gtagtcctgc gcgggatcgg gcgacagcat catcagcttg ccggtgtcgg tgtccgccac
    10981 accctgcggc acgtggcagg tgcggcagaa gcccaggttg ggctgcacgc gcgcggtgaa
    11041 gaaatccttg tcgctggcga acgtgctgcc tgcaccaccg ccggtcggtg tgccgcttcc
    11101 gccagcggat gcctgcggcg tgccgctgta cgttccgctg catgcaccca gcatcgccgc
    11161 ggccagcagc atcgcggcat gaatcttcat cggcatcgcg aatttcccct cgctgatgcc
    11221 catgcattgt gggcatgcgc ggtgcggccg gtcaggcggc gacacgccat cgtgcttgtc
    11281 atttcgggcc accggtgtag attggccgcc gatgaaacct gccgatccta acaagctgtg
    11341 gattcctgcc gtctacggcc tgctgatcat cgacctggtc gaacagcgcg gcgtgagccg
    11401 cacgaaactg ctcgacggca tcggcatacc cgacgcggca tggcgcgaag tcgacggccg
    11461 catttcgctg atccaggccg gcgccatgct gtacaagggc gtcaagctca ccggcgacgg
    11521 cgcgctgggc tacgagatcg gcctgtcgag caacctcacc acgcatggct tcgtcggcta
    11581 cggcctgctc agccacgcca ccgtgcgcga agcggtcctg tttggagcgg gcgtgctgcc
    11641 gctgcgcctg cccaatctct cgctgcgcct gttcgaggat ggcgcgcagg gcgtggtcga
    11701 cg
//
LOCUS       DASZZC010000018         6866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1186,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000018 DASZZC010000000
VERSION     DASZZC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VHE37_00440"
     CDS             complement(<1..232)
                     /locus_tag="VHE37_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HVT34020.1"
                     /translation="MVAALEQALAGGPLTAAQLATALSVSQPTVSRLLRSNAARVIVI
                     GRARNARYALRRSVRDLAADLPVYRIALDGSVR"
     gene            complement(361..1212)
                     /locus_tag="VHE37_00445"
     CDS             complement(361..1212)
                     /locus_tag="VHE37_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVT34021.1"
                     /translation="MTTRTLANVIPSIPASDGAGVKLRRSLGSRRGLYHDPFLMLDEF
                     YSDDPNDYLAGFPAHPHRGFETVTYMLDGHMRHEDNFGHRGDLGPGDVQWMTAAHGII
                     HSEMPQQTAGRMRGFQLWLNLPAAEKMQPADYRDIPSREIPSVALPGGVQVRVIAGVL
                     GAVSGPVNGADARKTTDPLYLDLHLPAAASADIPLAAGRNAFLYAYEGEVRVGGQLVP
                     HRAAATLGDGDAVTLTAGAQGARVLLLAARPLREPVVQYGPFVMNTHEQIEQAIADYQ
                     SGRLAAV"
     gene            complement(1246..1629)
                     /locus_tag="VHE37_00450"
     CDS             complement(1246..1629)
                     /locus_tag="VHE37_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT34022.1"
                     /translation="MKTIVNFSDLGGRFLLAAMFLVSGLSKIAGYAATQQYMAAQGVP
                     GALLPLVILTEVGGGALIVLGLWTRAAAVALAGFTVLAALLFHANFGDQVQQIMFMKN
                     LSIAGAFLMLAARGAGPLSLDARRA"
     gene            complement(1658..2260)
                     /locus_tag="VHE37_00455"
     CDS             complement(1658..2260)
                     /locus_tag="VHE37_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVT34023.1"
                     /translation="MKVLQINSSIHGAAGQSSVLAAQLARQFDAEVTVRDFTVEPVPH
                     LDAARFAAFASKPGQRSAAQQAVIDYSDRLIEEVRQADVLVLGVPMYNFSVPSQLKAW
                     FDHLARAGVTFKYSETGPVGLITGKKVYVISTRGGIHAGKPIDTETNYLRDFLAFLGM
                     TDVEFIYAEGLAYGEPVREQALATARASVARIVPALAYAA"
     gene            2361..3329
                     /locus_tag="VHE37_00460"
     CDS             2361..3329
                     /locus_tag="VHE37_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT34024.1"
                     /translation="MSQTLHITLDQWRALVAVVEAGGYAQAAESLHKSQSTLTYAVQK
                     IERLLGVKMFEIQGRKAVLTAAGQTLYRRGKALVDEAWQLEHAAGELAAGWEPELRLA
                     VDIVFPTWLLLQCFRSFAAERPQTRIELWETVLGGTEEALTEGRVQIAITSHVPPGFF
                     GEPLVHLKFIAAAAPMHPLHQLKRPLTQRDLKPHRHLVIRDSGAQRTRVAGWLNEQRW
                     TVSHKATSIRAAVMGLGYAWYGEEMIREELKSGQLKPLPLREGAERWGTLYLVFADAD
                     AAGPGARRLAEIIRTASAGCRAIDPALAQPAKPPKAAKKKTVRATS"
     gene            complement(3319..4356)
                     /gene="tdh"
                     /locus_tag="VHE37_00465"
     CDS             complement(3319..4356)
                     /gene="tdh"
                     /locus_tag="VHE37_00465"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HVT34025.1"
                     /translation="MQQASMQALVKAKPAAGLWLQDVAQPQIGPDEVLIRIRRMAICG
                     TDLHIYKWDEWAQRNVPVPTTIGHEYAGEIAAVGANVKRVEVGQRVSGEGHIVGETSR
                     NVRAGRFHLDPQTRGIGVNLPGAFAEYLKLPAFNAVPLPDYVDDEIGALLDPLGNAVH
                     TALAFELVGEDVLLTGAGPIGIMAAAVARFAGARHVVITDVNDFRLELARHVRGVRTV
                     NVAREELKAVMHELEMSEGFDVGLEMSGAPGALEQMVDAMIMGGKIALLGLPPGRTDT
                     DWSRIILKALTLKGIYGREMFDTWYKMLALIKAGLDLRFLITHRYRVADYQQAFDTAL
                     AGKAGKIVLSW"
     gene            4426..5283
                     /gene="purU"
                     /locus_tag="VHE37_00470"
     CDS             4426..5283
                     /gene="purU"
                     /locus_tag="VHE37_00470"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HVT34026.1"
                     /translation="MSTSDREYVLQFTSPDRPGVLAAITGALFEAGCNVRDAAQFGDP
                     ESQLFFVRMHVRTEGDVAAATLLRALEPARTRLRLQVECFDLAVKPRVLIAVSQHGHC
                     LHTLLHKWQLGTLPCEIVGVVSNHETFRSLVQWYELPFHFLPVGNDRPAQERALLQLA
                     RDQRTDLLVLARYMQVLSAEACAEFSGRCINIHHSFLPGFKGAKPYHQAHARGVKLIG
                     ATAHYVTTDLDEGPIIEQDVRRVTHATTPEEMVELGRETEASVLARAVRWHVEHRVVL
                     NGGKTLVFE"
     gene            complement(5287..6465)
                     /locus_tag="VHE37_00475"
     CDS             complement(5287..6465)
                     /locus_tag="VHE37_00475"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HVT34027.1"
                     /translation="MDAHLKRQLAQLEHDGLYKHERVISSMQAARITLDDGRSVINLC
                     ANNYLGLADAPELIAAAHAALDRYGVGMASVRFICGTQDAHKQLEAEIAQWLGTEDAI
                     LFPSAFDANGGVFEALLGEEDAIISDALNHASIIDGIRLCKAQRHRYANCDLAELESC
                     LQKTRGARFRMIATDGVFSMDGTIAPLDRICALAQQYDALVMVDDAHAHGVLGATGAG
                     SVEHRGMRGRVQVITGTLGKALGGASGGFVAASRPMVEWLRNRARPYLFSNSVAPALV
                     AAAREALRINRVRPQLRAQLIDNAAYWRAGLGKLGYTLQPGEHPIIPVMLGDAKLAGS
                     MSKRLLELGVYVIGFSYPVVPLGKARIRTQMSAALTRAELDEALAAFAQAGRELGVIR
                     "
     gene            complement(6637..>6866)
                     /locus_tag="VHE37_00480"
     CDS             complement(6637..>6866)
                     /locus_tag="VHE37_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34028.1"
                     /translation="GKRVHAVVAGHMHHRLKGGGLPRRVQLLRDHTLYLNAARVPRIR
                     VQDGAEVRYHVSLTLGAQDARATEIWVPTPK"
BASE COUNT         1119 a         2307 c         2349 g         1091 t
ORIGIN      
        1 cgcggaccga accgtccagc gcgatccggt acaccggcag gtcagcggcc aggtcgcgca
       61 cgctgcggcg cagggcgtag cgcgcattgc gcgcccgccc gatgacgata acgcgcgctg
      121 cgttcgaccg cagcagccgc gacaccgtgg gctggctcac gcttagtgcg gtcgccagct
      181 gcgccgcggt cagtggcccg ccggccagcg cttgctccag ggcagcaacc atgaaaatca
      241 actagatcta tgaatagata atgaatagat actagccgac tgagtcgttc cggttcaatg
      301 cattggaccg ccgcccgcca tcttcatgca tggattcgag ccgcacgcac gggccgccgc
      361 tcaaaccgcc gccagcctgc cgctctggta atcggcgatg gcctgctcga tctgctcgtg
      421 cgtgttcatc acgaacgggc cgtactgcac caccggttcg cgcagcggcc tggccgcgag
      481 cagcagcacg cgcgcgccct gcgcgccggc ggtgagcgtc accgcgtcgc cgtcgccgag
      541 cgtggccgcg gcgcggtgcg gcaccagctg gccgccgacg cgcacttcgc cttcataggc
      601 gtagagaaag gcgttgcggc ccgcggcaag cgggatgtcc gcagacgcag cggccggcag
      661 gtgcaggtcg agatacagcg ggtcggtggt cttgcgcgca tccgcgccgt tgaccggccc
      721 ggagacagcg ccgagcacgc cggcgatgac gcgcacctgc acgccgccag gcagcgccac
      781 cgacggaatt tcgcgcgatg gaatgtcgcg gtaatccgcc ggctgcatct tctccgccgc
      841 cggcaggttg agccacagct ggaagccgcg catgcgccct gccgtctgct gcggcatctc
      901 ggaatggatg atgccgtgcg cagcggtcat ccactgcacg tcgccggggc cgaggtcgcc
      961 gcgatggccg aagttgtcct cgtggcgcat gtggccgtcg agcatgtagg tcaccgtctc
     1021 gaagccgcgg tgcggatgcg cggggaagcc ggcgagatag tcattggggt cgtcggaata
     1081 gaactcgtcg agcatcagga acgggtcgtg gtacagcccg cgccgcgagc ccaggctgcg
     1141 gcgcagcttg acgccggcgc cgtcggacgc ggggatcgac gggatcacgt tggcgagggt
     1201 gcgggtggtc atgggaaggc tccggtgaga tgaaacaaga tggggtcagg cgcgtcgcgc
     1261 atcaagcgac agcggcccgg caccgcgcgc ggcaagcatc aggaacgcgc cggcgatcga
     1321 caggttcttc atgaacatga tctgctggac ctggtcgccg aaattggcgt gaaacagcag
     1381 agcggccagc acggtgaagc cggccagcgc gacggcggcg gcgcgcgtcc acagtccgag
     1441 cacgatcagc gcaccaccgc caacctcggt gaggatgacc agcggcagca gcgcgccggg
     1501 cacgccctgc gccgccatgt actgctgcgt ggcggcgtag ccggcgatct tgctcaggcc
     1561 ggagacgagg aacatggcgg cgagcaggaa gcgtccgccc agatcggaaa agttgacgat
     1621 ggttttcatg gttgaactcc ttggttgagg ggatggatca ggcggcgtaa gccagcgcgg
     1681 gaacaatgcg cgccacgctg gcgcgcgcgg tggccagcgc ctgctcgcgc accggctcgc
     1741 cgtaggccag gccctcggcg tagatgaact ccacgtcagt catgccgagg aaggcgagga
     1801 agtcgcgcag gtaattcgtc tcggtgtcga tcggcttgcc ggcatggatg ccgccgcgcg
     1861 tggaaatcac gtagaccttc ttgccggtga tcagccccac cgggccggtc tcgctgtact
     1921 tgaaggtgac gcccgcgcgc gccaggtggt cgaaccaggc tttcagctgc gacggcacgc
     1981 tgaagttgta catcggcacg ccgagcacca gcacatcggc ctggcgcacc tcctcgatca
     2041 ggcggtcgga atagtcgatc accgcctgct gcgcggcact gcgctgcccc ggcttgctgg
     2101 cgaaggcggc gaagcgcgcc gcatccaggt gcggcaccgg ctcgacggtg aaatcgcgca
     2161 cagtgacttc ggcgtcgaac tgccgcgcca gctgggcggc aagcaccgag gactggccgg
     2221 cggcgccgtg gatgctgctg ttgatctgca ggactttcat gactcactcc gctgtttttg
     2281 gggtgagcca ttaaatagtt gcttcaatcg ataaaaaagc ggtatttatc gcgcctattt
     2341 atcgaatcat tggatagctg atgagccaga ccctgcatat caccctcgat caatggcgcg
     2401 cgctggtcgc ggtggtggaa gccggcggct acgcccaggc ggcggaatcg ctgcacaaga
     2461 gccagtccac gctgacctac gcggtgcaga agatcgaacg cctgctcggt gtgaagatgt
     2521 tcgagatcca gggacgcaag gcggtgctca ccgctgccgg gcagacgctg taccggcgcg
     2581 gcaaggcgct ggtcgacgaa gcctggcagc tcgagcacgc ggcgggcgag ctcgcagccg
     2641 gctgggagcc ggaactgcgg ctggcggtgg acatcgtctt tcccacctgg ctgctgctgc
     2701 aatgcttcag gagcttcgcc gccgagcggc cgcagacgcg catcgagttg tgggagaccg
     2761 tgctcggcgg caccgaggag gcgctcaccg agggccgcgt gcagatcgcc atcacctcgc
     2821 acgtgccgcc gggattcttc ggcgagccgc tggtgcacct gaagttcatc gccgctgccg
     2881 cgcccatgca cccgctgcac cagctgaagc gtccgctgac ccagcgcgac ctcaagccgc
     2941 accggcacct ggtgatccgc gactccggcg cgcagcgcac gcgcgtggcc ggctggctca
     3001 acgagcagcg ctggacggtg tcgcacaagg ccacctcgat ccgcgccgcg gtgatgggcc
     3061 tgggctacgc gtggtacggc gaggagatga tccgcgagga gctgaagagc ggccagctga
     3121 aaccgctgcc gctgcgcgaa ggcgccgagc gctggggcac gctgtacctg gtgtttgccg
     3181 atgccgatgc tgccggaccc ggcgcacggc ggctggcgga gatcatccgc acggcgtcag
     3241 cgggttgccg cgccatcgat cccgcgctgg cgcagccggc aaaaccgccg aaggccgcaa
     3301 agaaaaaaac ggtgcgtgct accagctgag cacgatcttg ccggccttgc cggccagcgc
     3361 ggtgtcgaac gcctgctggt aatccgccac gcggtagcgg tgcgtgatga ggaagcgcag
     3421 gtcgagcccg gccttgatca gcgccagcat cttgtaccag gtatcgaaca tctcgcggcc
     3481 gtagatgccc ttgagcgtga gcgccttgag gatgatgcgc gaccagtcgg tgtcggtcct
     3541 gcccggcggc aggccgagca gcgcgatctt gccgcccatg atcatggcgt cgaccatctg
     3601 ctccagcgcg cccggcgcgc cgctcatctc caggccgacg tcgaagcctt ccgacatttc
     3661 cagctcgtgc atcactgcct tcagctcttc gcgcgccacg ttcaccgtgc gcacgccgcg
     3721 cacgtggcgc gccagctcca ggcggaaatc gttgacgtcg gtgatgacca cgtggcgcgc
     3781 gccggcaaag cgcgccaccg ccgcggccat gatgccgatc ggcccggcgc cggtgagcag
     3841 cacgtcctcg cccaccagct cgaaggccag cgcggtgtgc accgcgttgc ccagcgggtc
     3901 gagcagcgcg ccgatctcgt cgtcgacgta atcgggcaag ggcaccgcgt tgaacgccgg
     3961 cagcttgagg tactcggcaa aggcgccggg aagattcacg ccgatgccgc gcgtctgcgg
     4021 atcgagatgg aatcgtccgg cgcgcacgtt gcggctggtc tcgccgacga tgtggccctc
     4081 gcccgacacg cgctggccga cctcgacgcg cttgacgttg gcgcccaccg ccgcgatctc
     4141 gccggcgtat tcgtggccga tggtggtggg caccggcacg ttgcgctgcg cccactcgtc
     4201 ccacttgtag atgtgcagat cggtgccgca gatggccatg cggcggatcc tgatcagcac
     4261 ctcgtcggga ccgatctgcg gctgcgccac gtcctgcagc cacagcccgg cggcgggttt
     4321 tgccttgacc agcgcttgca tcgacgcctg ttgcatggcc tatcctcctg atcgcctttc
     4381 cgtagtacta tacagatatg tatttaccgt aaccaccatc gagccatgtc cacatccgac
     4441 cgcgaatacg tgctgcagtt tacgtcgccc gaccgccccg gcgtgctcgc cgccatcacc
     4501 ggcgcgctgt tcgaggccgg ttgcaacgtg cgcgatgccg cgcagttcgg cgacccggaa
     4561 tcgcagctgt tcttcgtgcg catgcacgtg cgcacggaag gcgacgtggc cgctgccacg
     4621 ctgctgcgcg cgctggagcc ggcgcgcacg cgcctgcgcc tgcaggtgga atgcttcgac
     4681 ctcgcggtga agccgcgcgt gctgatcgcg gtgtcgcagc acggccactg cctgcacacg
     4741 ctgctgcaca agtggcagct cggcacgctg ccgtgcgaga tcgtcggcgt ggtgtccaac
     4801 catgagacct tccgctcgct ggtgcagtgg tacgaactgc cgttccactt cctgccggtg
     4861 ggcaacgaca ggccggcgca ggagcgcgcg ctgctgcagc tggcgcgcga ccagcgcacc
     4921 gacctgctgg tgctggcgcg ctacatgcag gtgctgtcgg ccgaggcctg cgcggaattc
     4981 tccgggcgct gcatcaacat ccaccactcg ttcctgcccg gtttcaaggg cgccaagccg
     5041 taccaccagg cgcatgcacg cggggtcaag ctgatcggcg ccaccgcgca ttacgtcacc
     5101 accgatctcg acgagggccc gatcatcgag caggacgtgc gccgcgtgac gcacgccacc
     5161 acgccggagg aaatggtcga gctgggccgc gagaccgagg ccagcgtgct ggcgcgcgca
     5221 gtgcgctggc acgtcgagca ccgcgtggtg ctcaatggcg gcaagacgct ggtattcgaa
     5281 taggcgtcag cggatcacgc ccagctcgcg cccggcctgg gcaaaagcgg cgagcgcctc
     5341 gtccagttcc gcgcgcgtga gcgcggccga catctgcgtg cggatgcgcg ccttgcccag
     5401 cggcaccacc gggtaggaaa agccgatgac gtacacgccg agctccagca gccgcttcga
     5461 catgctgccg gcaagcttcg catcgcccag catcaccggg atgatcgggt gctcgcccgg
     5521 ctgcagcgtg tagccgagct tgcccagccc cgcgcgccag taagcggcgt tgtcgatcag
     5581 ctgcgcgcgc agctgcggac gcacgcggtt gatgcgcagc gcctcgcgcg cggcggcgac
     5641 cagcgccggc gccacgctgt tggagaagag atagggccgc gcgcggttgc gcagccattc
     5701 caccatgggc cggctggcgg cgacgaaacc acccgaagcg ccgccgaggg ccttgcccag
     5761 cgtgccggtg atcacctgca cgcggccgcg catgccgcga tgctccacgc tgccggcgcc
     5821 ggtggcgccg agcacgccgt gcgcatgcgc gtcatccacc atcaccagcg cgtcgtactg
     5881 ctgcgccagc gcacagatgc ggtcgagggg cgcgatggtg ccatccatgg aaaacacgcc
     5941 gtcggtggcg atcatgcgga agcgcgcgcc gcgggttttc tgcaggcagg attcaagctc
     6001 ggccaggtcg cagttggcgt agcggtgccg ctgcgccttg cacaggcgga tgccgtcgat
     6061 gatcgacgcg tggttgagcg cgtcggagat gatcgcgtct tcctcgccca gcagcgcctc
     6121 gaacacgccg ccattggcgt cgaacgcgga cgggaacagg atcgcgtcct cggtgccgag
     6181 ccattgcgcg atctcggctt cgagctgctt gtgcgcgtcc tgcgtgccgc agatgaagcg
     6241 caccgaggcc atgccgacgc cgtagcggtc cagcgcggcg tgcgcggcgg cgatcagttc
     6301 cggcgcgtcg gccaggccca ggtaattgtt ggcgcacagg ttgatcaccg agcgcccatc
     6361 gtcgagcgtg atgcgcgcgg cctgcatcga gctgatcacg cgctcgtgct tgtacaggcc
     6421 gtcgtgttcg agctgcgcca gctggcgctt caggtgagcg tccacgtgtc actccttggt
     6481 attgcggcgc gcaccgtaac gcagggcggc caaaaatgcc tgtaccgcac tgccttccat
     6541 catgttatta attatcgtct gtttttatat ttgatcaata attaataaca ccatagacgg
     6601 ccatgcggtg aggctgtttt tgcgccacgc gccgcgctac ttcggcgtcg gcacccagat
     6661 ctcggtggcg cgcgcgtcct gcgcgccgag cgtgagcgac acgtggtagc gcacctcggc
     6721 gccgtcctgc acgcggatgc gcggcacgcg cgcggcattg agatacagcg tgtgatcccg
     6781 cagcagctgc acgcgccgcg gcaagccgcc gcccttgagc cggtgatgca tgtggccggc
     6841 gacgaccgcg tgtacgcgct tgccct
//
LOCUS       DASZZC010000019         9380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_386131,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000019 DASZZC010000000
VERSION     DASZZC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386131"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VHE37_00485"
     CDS             complement(<1..1122)
                     /locus_tag="VHE37_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34029.1"
                     /translation="MQPTHSQFALLGTRRFLPFFATQTLGAFNDNVFKNALVIFVTFG
                     IAGLSAAQESLYVNLAAGLFILPFFLFSATAGQIAEKYEKAMLIRNIKLLELGIAVLA
                     AFGFALGSLPLLFGVLFLLGLQAALFGPIKYAILPQALREDELVGGNALVETATSLAI
                     LLGSLLGGVLMAMAHGRGLVSAAVLVIAAAGYLASRGIPAAVPMAPSLVINWNPLTET
                     LANLRFMRGNRPVLLSVMGISWFWFFGATFLAQLPGYTKQYLGGSEQVVTLLLTVFSI
                     GTAAGSLLCERLSGHKVEIGLVPLGSIGLTLFGVDLYFAHPGAAAEQGLTAAQFLHVL
                     GNHRVLWDLGLIGLFAGFYIVPLYSMVQQRSEASHRSRVI"
     gene            complement(1197..2012)
                     /locus_tag="VHE37_00490"
     CDS             complement(1197..2012)
                     /locus_tag="VHE37_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT34030.1"
                     /translation="MIVDAWIQHPTLRFAQQPMFESLWRWTKQRPPSSEVPLEFTLAT
                     MKQAGVDHALVSAWYGPQGALISNDEVAHWVAQSKGMLHGVCSVDLMRPMEAVRELRR
                     CVRERGFKALRMLPWWWNLPPNDRHYYPLYAECCELGVPFCLQVGHTGPLMPSEPGRP
                     IPYLDQVALDFPELTIVGGHIGYPWTQEMIALATKYEHVYIDTSAYTARRYPPELVAY
                     LRGHGRRKVLFGSNYPMIAPADCLKELDSLGLDPGARADFLGGNAQRVFRLAA"
     gene            complement(2165..2842)
                     /locus_tag="VHE37_00495"
     CDS             complement(2165..2842)
                     /locus_tag="VHE37_00495"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT34031.1"
                     /translation="MPKVVLHQWDMSPFCNKVRRCLKYKGVDYSIVNYNGLLALNAAK
                     LSKVGKLPVLDWDNERVQDSSAIARFLDAKVPARPLYPKDDPQALAAGRMWEDWAGQS
                     LYFYEIYFRMLDPVSLERALDLIADGRPRYERAILKAVFKRRYPRKLKEQGLARLEPA
                     EVERQFFERLEDIEYTLEGRPFLTGLSPSIGDFSVAAQLDELIRTSDLRERILAFPRL
                     KAWLSRC"
     gene            complement(2938..4281)
                     /locus_tag="VHE37_00500"
     CDS             complement(2938..4281)
                     /locus_tag="VHE37_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVT34032.1"
                     /translation="MKIKSRRDFLRAVVHGGGALGAMSAFGPMAALASSGSGYQALVC
                     VFLAGGNDGHNTVVPIATAQQGYSVYQQARKSLALPQNQLLPISSNGDSYGLHPALSE
                     IQALYNGGQAAVLANVGNLVQPLNRQSYLTNNSAIIPTALFSHSDQTSQWQSASPTGT
                     LPTGWGGRIMDLLQPQNSGSVFPPMTSTGGCGIFCSGAQVFPATVPPSGMAMLDSHAA
                     GASGASAQTQLLSFDNGMTLVQAANSILTRGNQYATTLSGLLASSNLQTTFPANNPLA
                     AQLKTVAQIMSVRNSLGLTRQIFFCQLGGFDTHSDQTETQALLLGQLSKAVDAFYKAT
                     QELGIESSVTTFTASEFGRTLQPSGTDGSDHAWGSHHFIIGGSVLGGKIYGTFPQLVL
                     GSSQDATGRGSLIPTSAVAQYGATLAGWFGVDSGSMAGIFPNIGNFGSANLGFLG"
     gene            complement(4296..7112)
                     /locus_tag="VHE37_00505"
     CDS             complement(4296..7112)
                     /locus_tag="VHE37_00505"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HVT34033.1"
                     /translation="MRDTLKRYAMLLCLGLLAICGPVQAEETGGMPPPPSPKVTISPG
                     YTSVGVNGTVQYTATVTGLAKKHVTWYVSGKHGGGPVNGTISKTGLYTAPAKIPKNGV
                     TISALASDGKTQAIVYVNVAPPGPPISAVSPSPIPVGTRSITVSGTGFVKDAIIRLND
                     VSLTTTFINSTTLTASEYTGAAGSGVVRVMNPGSLWSDGYTVTFTGSTGSGDNRQTIS
                     PASASLKLTATQQFTSAGATSWSATAGTITSNGFYTAPSTMPASSTVTVSATGPKGTA
                     NATVTLVSGTPQTVAPSSASLALGATQQFTSSGATSWSATAGSISASGLYTAPAAMPA
                     SNSVTVSAQGPNGTGNATVTLIPPTPVISSVGNANLPLGSFTTTLSGSGFIAQSVARL
                     NGSALTTAYANGQLTVSGYAAQSGTANFTVSNYNVTSQPYAVQVGVANAQVTAAAARR
                     FLQQAAFGPTPSDAAHVQQVGFQGWLSEQYAGAQVSNYDSVTGSQGGLPAHFMTNAVS
                     NADQLRQRVAFALSQIFVISLNKLIWNDNMVLYQDMLLKDAFGNYRQIMEDVTLSPGM
                     GQFLDMANNAKANPAAGSLANENYARELMQLFTIGTSVLASDGTVQYDSNHVPVPTYT
                     QPTIAEFARVFTGWTYAPDPGKPVQWGAYITSNGPMVPYPAQHDSGSKQLLNGAVAPS
                     GLTPQQDLDFALDNIFNHANVGPFVGKQLIQHLVKSNPSPAYIGRVAAAFNDNGHGVR
                     GDMQAVINAVLLDSEARANDEGGADQATDGHLQEPALFIAGMVRAFGGQMNDQNYWPS
                     ELAALNQDLFDAPSVFNYYSPNYRVPGSGGLSGGEFQLDTPNNAIVRDNLVAGLMFSQ
                     YQKPVQSYGPGTTVDLTPFVQLASNPATLVDALDLTLTRGVMPSSMKQALVTAVTNDG
                     SGNVHRVQMAAYLILSSSYYNVWH"
     gene            7544..9049
                     /locus_tag="VHE37_00510"
     CDS             7544..9049
                     /locus_tag="VHE37_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT34034.1"
                     /translation="MDDPRDLTLALKSQFPIITIDTREEARVLALIERVANMEMWPLF
                     VWSVARGLHRAGANADIKAPYEADPEATRELPAALRQMIASPQHGVFVLLDPKPYLED
                     PVAQRLVKEFAQNSDNGRRTLVLIGADSGLPQELRRLAATFALAMTDAAQIKKIFQEE
                     IDLAQQQSLYARIRGDHDAAQLLVRHLVGMCAEDARRLIRQAVRDDGIIDMNDVARVL
                     KYKHEALGQGGVLQLEVDCGNFAQVAGLANLKHWLELRKAVFLEDGSDGGLDPPRGLL
                     LLGVQGSGKSMAAKAVAGSWGVPLLRMDFGALYSKWSGETEKNLRDSLKVADAMEPAV
                     LWIDEIEKGLSAEAGEADGGMSRRVLGTLLTWMNERKSRVFLAATANDIATLPPELMR
                     KGRFDEIFFVDLPDDATRAEIFRRQLARRKQAPEGFDLRALAAKSVGFSGAEIEQAVV
                     SALYAAKASHQPMSSALVAEELARTRPLSVVMAEKIAALRAWASSRTVPAN"
     gene            9145..>9380
                     /locus_tag="VHE37_00515"
     CDS             9145..>9380
                     /locus_tag="VHE37_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT34035.1"
                     /translation="MDAKTGAGDLLAPLKKHFGFDAFRPGQEAVVRDALAGRDLLAVM
                     PTGGGKSLCFQLPALLTDGVMIVVSPLIALMQDQV"
BASE COUNT         1548 a         3054 c         3196 g         1582 t
ORIGIN      
        1 gatcacgcgc gagcggtgcg aggcctcgct gcgctgctgc accatggaat acagcggcac
       61 gatgtagaag ccggcgaaca ggccgatcag gcccaggtcc cacagcacgc ggtgattgcc
      121 cagcacgtgc aggaactgcg cggccgtcag gccctgttcg gccgccgcgc ccggatgggc
      181 gaaatatagg tcgacaccaa acaacgtgag tccgatcgag cccagcggca ccagcccgat
      241 ctccaccttg tggccggaca ggcgctcgca cagcagcgag ccggccgcgg tgccgatgga
      301 aaacaccgtg agcagcagcg tcaccacctg ctcgctgccg ccgagatact gcttggtgta
      361 gcccggcagc tgcgcgagaa acgtggcgcc gaagaaccag aaccaggaaa tgcccatcac
      421 cgacagcagc accggccggt tgccgcgcat gaagcgcagg ttggccagcg tctcggtgag
      481 cgggttccag ttgatgacga gactcggcgc catcggcacc gccgccggaa tgccgcggct
      541 ggcgagatag ccggcggcgg caatcaccag caccgctgca ctgaccaggc cgcggccgtg
      601 cgccatggcc atcagcacgc cgccgagcag gctgcccagc aggatggcga gcgaagtcgc
      661 ggtctccacc agcgcgttgc cgccgaccag ctcatcctcg cgcagcgcct gcggcaggat
      721 ggcgtacttg atcggcccga acagcgccgc ctgcaggccg agcaggaaca gcacgccgaa
      781 cagcagcggc aggctgccga gggcaaagcc gaacgcggcc agcaccgcaa tgcccagctc
      841 cagcagcttg atgttgcgga tcagcatggc cttctcgtac ttctcggcga tctggcccgc
      901 ggtggcggaa aacaggaaga acggcaggat gaacaggccc gccgccaggt tcacgtacag
      961 gctttcctgc gccgcgctca gcccggcgat gccgaaggtg acgaagatca ccagcgcgtt
     1021 cttgaacacg ttgtcgttga acgcgcccag cgtctgcgtg gcaaagaacg gcaggaaacg
     1081 gcgcgtgccc agcagcgcga actggctgtg cgtcggttgc atcacccatc ctccccgcga
     1141 atggcgacgg aattccgcgt caataataaa cgatgttcat ttatacgcaa gccgcgtcag
     1201 gcggcgaggc ggaatacgcg ctgtgcgttg ccgccgagga agtcggcgcg cgcgccgggg
     1261 tccaggccga gagaatcgag ttccttcagg caatcggccg gcgcgatcat cgggtagttg
     1321 ctgccgaaca ggactttcct gcgcccgtgc ccgcgcaggt aagcgaccag ctcgggcgga
     1381 tagcgccgcg cggtatacgc ggaggtatcg atgtacacat gctcgtactt ggtggcgagc
     1441 gcgatcatct cctgcgtcca cggatagccg atgtgcccgc cgacgatggt gagctcagga
     1501 aaatccagcg ccacctggtc caggtacggg atcggccggc ccggctccga cggcatcagc
     1561 ggcccggtgt ggcccacctg caggcagaac ggcacgccaa gctcgcagca ctcggcgtac
     1621 agcgggtagt agtggcgatc gttcgggggc aggttccacc accatggcag catgcgcagt
     1681 gccttgaacc cgcgctcgcg cacgcagcgg cgcagttcgc gcaccgcctc catcggccgc
     1741 atcaaatcga ccgagcacac gccgtgcagc atgcccttcg actgcgccac ccaatgagcg
     1801 acttcgtcgt tggagatcag cgcgccctgc gggccgtacc aggcgctgac cagcgcgtga
     1861 tccacgccgg cctgcttcat cgtggcaagg gtgaattcca gcggaacctc ggaagacggt
     1921 ggacgttgct tggtccagcg ccagagcgac tcgaacatcg gctgctgcgc gaagcgcagc
     1981 gtcgggtgct ggatccaggc gtcgacgatc atcggtcagc cccaagaatc tccagaaagc
     2041 gtcgcgagcg atggcagttc gcgcgaggac gcagcgcagg aagcggagca tacacgccag
     2101 tatgtgagga ttccgagcac cgcctcgcgc gaaatgccgg gcgcagcagc tttccggaga
     2161 ttcttcagca gcgtgacagc cacgccttaa gccgcggaaa cgccaggatg cgctcgcgca
     2221 ggtcgctggt gcggatcagc tcgtcgagct gggcagccac ggaaaaatcg ccgatcgaag
     2281 gcgacagccc ggtgaggaat ggccggcctt ccagcgtgta ctcgatgtcc tccaggcgct
     2341 cgaagaactg ccgctccact tccgccggct ccagccgcgc caggccctgc tccttgagct
     2401 tgcgcgggta gcggcgcttg aacacggcct tgaggatggc gcgctcgtag cgcggacgac
     2461 cgtcggcgat caggtcgagc gcgcgctcga gcgacaccgg gtcgagcatg cggaaataga
     2521 tctcgtagaa atacagcgac tgcccggccc agtcctccca catgcgtccg gcggccagcg
     2581 cctgcggatc gtccttcggg tacagcggcc gcgctggaac cttcgcatcg agaaagcgcg
     2641 cgatggccga ggagtcctgc acgcgctcgt tgtcccagtc cagcaccggc agcttgccca
     2701 ccttcgacag cttggccgcg ttgagcgcca gcaggccgtt gtagttgacg atggagtaat
     2761 cgacgccctt gtacttcagg cagcgccgca ccttgttgca gaagggcgac atgtcccatt
     2821 ggtgcagcac gaccttgggc atgggattcc ttgttcttgt gtcagtgcgc ggattgtacc
     2881 gagcgtcatc gcccccgtgg ttgtctgcgc gcagccgagc tcatcacgcg ttcattgcta
     2941 gccgaggaag ccgaggttgg cgctgccgaa gttgccgatg ttggggaaga tgcccgccat
     3001 gctgccgctg tccaccccga accagccggc cagcgtggcg ccgtactgcg ccacagcgga
     3061 ggtcgggatc agcgagccgc ggccggtggc atcctggctg ctgcccagca ccagctgcgg
     3121 gaaggtgccg tagatcttcc cgccgagcac gctgccgccg atgatgaaat ggtggctgcc
     3181 ccacgcatga tccgaaccat cggtgccgct gggctgcagc gtgcggccga attctgacgc
     3241 ggtaaacgtc gtcacgctgg actcgatgcc cagctcctgc gttgccttgt agaacgcgtc
     3301 gaccgccttg ctcagctggc cgagcagcag tgcctgcgtc tcggtctggt cgctgtgcgt
     3361 gtcgaacccg ccgagctggc agaagaagat ctgccgcgtg aggcccagcg aattgcgcac
     3421 cgacatgatc tgcgccacgg tcttgagctg cgccgccagc gggttgttgg ccgggaaggt
     3481 ggtctgcagg ttgctgctgg cgagcagacc ggacagggtg gtggcgtact ggttgccgcg
     3541 cgtgaggatg ctgttggccg cctgcaccag cgtcatgccg ttgtcgaacg acagcagctg
     3601 cgtctgcgcg gaggcgccgg acgcaccggc cgcatgcgag tcgagcatgg ccatgccgct
     3661 cggcggcacc gtggcgggaa acacctgcgc gccgctgcag aagatgccgc agccgccggt
     3721 cgaggtcatc ggcggaaaca cgctgccgga attctgcggc tgcagcaggt ccatgatgcg
     3781 cccgccccag ccggtgggca gcgtgccggt gggcgacgcg ctctgccact ggctggtctg
     3841 gtcggagtgc gagaacagcg cggtggggat gatcgcgctg ttgttggtga ggtaactctg
     3901 ccggttgagc ggctgcacca ggttgccgac gttggccagc accgcggcct ggccgccgtt
     3961 gtatagcgcc tggatttcgg acagcgccgg gtgcaggccg tagctgtcgc cgttgctgct
     4021 gatcggcagc agctggttct gcggcagcgc cagcgatttg cgcgcctgct ggtacacgct
     4081 gtagccctgc tgtgcggtgg cgatcggcac cacggtgttg tggccgtcgt tgccgccagc
     4141 caggaacacg cacaccagcg cctggtaacc cgagcccgaa gaggccagtg ccgccatcgg
     4201 cccgaaggcg ctcatcgcgc cgagcgcgcc gccgccgtgc accacggccc gcaggaaatc
     4261 gcgcctggat tttattttca tggtggtgct ttccttcagt gccacacgtt gtagtaggag
     4321 gaggacagga tcaggtacgc cgccatctgc acgcggtgca cgttgcccga gccgtcgttg
     4381 gtcacggcag tgaccagcgc ctgcttcatc gacgacggca tcacgccgcg ggtcagggtg
     4441 aggtcgagcg catcgaccag cgtcgccggg ttcgacgcca gctgcacgaa cggcgtgaga
     4501 tccaccgtgg tgcccgggcc gtagctctgc accggcttct ggtactggct gaacatcagt
     4561 ccggcaacca ggttgtcgcg cacgatggcg ttgttcggcg tatccagctg gaattcgccg
     4621 ccggagagcc cgccgctgcc cggcacgcgg taattcggcg agtagtagtt gaacacgctg
     4681 ggcgcgtcga acaggtcctg gttcagggcc gccagttcgc tcggccagta gttctggtcg
     4741 ttcatctggc cgccgaacgc gcgcaccatg ccggcgatga acagcgcggg ttcctgcagg
     4801 tggccgtcgg tcgcctggtc ggcgccgccc tcgtcgttgg cgcgcgcctc gctgtcgagc
     4861 agcaccgcgt tgatcaccgc ctgcatgtcg ccgcgcacgc cgtggccgtt gtcgttgaac
     4921 gccgcggcca cgcggccgat gtacgccggg ctcgggttgc tcttcaccag gtgctggatc
     4981 agctgcttgc ccacgaacgg tccgacgttg gcgtggttga agatgttgtc gagcgcgaag
     5041 tcgagatcct gctgcggcgt cagcccggac ggtgccaccg cgccgttgag cagttgcttg
     5101 ctgccgctgt cgtgctgcgc cgggtacggc accatcggcc cgttgctggt gatgtacgcg
     5161 ccccactgca ccggcttgcc cgggtcgggc gcgtaggtcc agccggtgaa cacgcgcgcg
     5221 aattccgcga tggtcggctg cgtgtaggtg ggcaccggca catggttgct gtcgtactgc
     5281 acggtgccgt cgctggccag cacgctggtg ccgatggtga acagctgcat caactcgcgc
     5341 gcgtaattct cgttggccag cgaaccggcc gcgggattgg ccttggcgtt gttggccatg
     5401 tcgaggaact ggcccatgcc cgggctcagc gtcacgtctt ccatgatctg ccggtagttg
     5461 ccgaaagcgt ccttcagcag catgtcctgg tacagcacca tgttgtcgtt ccagatcagc
     5521 ttgttcagcg agatgacgaa gatctggctc agcgcgaagg ccacgcgctg gcgcaattga
     5581 tcggcgttgc tcaccgcgtt ggtcatgaaa tgcgccggca agccgccctg gctgccggtg
     5641 acgctgtcgt agttggacac ctgcgcgccg gcgtactgct cggacagcca gccctggaag
     5701 ccgacctgct gcacgtgcgc ggcgtcgctg ggcgtcggcc cgaacgccgc ctgctgcagg
     5761 aagcgccgcg ccgccgcggc cgtcacctgc gcgttggcca cgcccacctg caccgcgtac
     5821 ggctgcgagg tgacgttgta gttgctcacg gtgaagttcg ccgtgccgct ctgcgccgcg
     5881 tagccgctca ccgtcaactg gccgttggca taagcggtgg tcagcgccga gccgttgagc
     5941 cgggccacgg attgcgcgat gaacccgctg ccggacaagg tggtggtgaa gctgcccagc
     6001 ggaaggttgg cgttgcccac gctgctgatc accggcgtcg gcggaatcag cgtcaccgtc
     6061 gcgttgccgg tgccgttggg gccttgcgcg ctgacggtca cgctgttcga cgccggcatc
     6121 gccgccggcg ccgtgtacag gcccgaggca ctgatgctgc cggcggtcgc gctccatgac
     6181 gttgcgccgc tggaggtgaa ctgctgcgtg gcgcccagcg ccagcgacgc gctcgacggc
     6241 gccaccgtct gcggcgtgcc gctgaccagc gtcaccgtgg cgttcgccgt gcccttgggg
     6301 ccggtggcgc tcaccgtcac cgtgctcgag gccggcatgg tcgacggcgc ggtgtagaaa
     6361 ccattcgacg taatggtgcc ggcggtggcg ctccacgagg tggcgccggc gctggtgaac
     6421 tgctgcgtgg cggtgagctt cagcgaggcc gacgccggcg agatggtctg gcggttgtcg
     6481 ccgctgccgg tgctgccggt gaacgtgacg gtgtagccgt cgctccacag cgagcccgga
     6541 ttcatcacgc ggaccacgcc gctgccggct gcgccggtgt actcgctcgc cgtcagcgtc
     6601 gtgctgttga tgaacgtagt ggtgaggctg acgtcgttga gccggatgat cgcgtccttg
     6661 acgaagcccg tgccgctgac ggtgatcgag cgggtgccga ccgggatcgg gctgggcgat
     6721 accgcgctga tcggcgggcc tggcggcgcc acgttgacgt agacgatcgc ctgcgtcttg
     6781 ccgtcggagg ccagcgcgct gatggtgacg ccgttcttcg gaatcttggc cggcgccgtg
     6841 tacaggccgg tcttgctgat ggtgccgttc accgggccgc cgccatgctt gccgctcacg
     6901 taccaggtca cgtgtttctt ggccaggccg gtgacggtgg ccgtgtactg caccgtgccg
     6961 ttgacgccca ccgaggtgta gcccgggctg atggtcacct tgggcgatgg cggcggcggc
     7021 ataccaccgg tctcctcggc ctgcaccggc ccgcagatcg ccagcaggcc caggcacagc
     7081 agcatcgcgt aacgcttcaa ggtgtcgcgc atcatcggca tccctttact tttattggta
     7141 ggcaccgcgc cgatcgtctg cctcactcct gcctgacgat ggcgccggct tgtccaccgg
     7201 cgatgcgact ttatttaccg gtatgcggtg caaatgtgcg ctccatcaag ctcgccgtgt
     7261 ggcgccgatg gacggcagtg cgagcaccgt cgaacgtgaa ttcgccgtca ggttgctctc
     7321 agctgtgcgt gaattgcgtc acgcgatact cgcggcgcgt gcgcaaaaaa cgtccaccgc
     7381 accgcattcc agcgagttat aaaatattta ccgtttcact ttttgattaa ttttttataa
     7441 caacgaaaag cagcatgcgg tgacctctta aatgcgtgcc tcggcgcgca agcctgcacg
     7501 cggccgcgcc ggtaaaatcc tccgcgtctg ggggagacat tcaatggacg acccgcgcga
     7561 tctcacgctg gcgctcaaat cgcagttccc gatcatcacc atcgacacgc gggaagaagc
     7621 gcgcgtactg gcgctgatcg agcgcgtggc caacatggag atgtggccgc tgttcgtgtg
     7681 gagcgtggcg cgcgggctgc accgcgccgg cgccaacgcc gacatcaagg cgccgtacga
     7741 ggccgatccc gaagccacgc gcgaactgcc ggcggcgctg cgccagatga tcgccagccc
     7801 gcagcacggc gtgttcgtgc tgctcgatcc caagccctac ctcgaagacc cggtggcgca
     7861 gcggctggtg aaggaattcg cgcagaacag cgacaacggc cggcgcacgc tggtgctgat
     7921 cggcgccgac agcgggctgc cgcaggaact caggcgcctg gccgccacct tcgcgctggc
     7981 catgaccgac gccgcgcaga tcaaaaaaat cttccaggag gaaatcgacc tggcgcagca
     8041 gcagtcgctg tacgccagga tccgcggcga ccacgacgcc gcgcagctgc tggtgcgcca
     8101 cctggtcggc atgtgcgcgg aagacgcgcg ccggctgatc cgccaggccg tgcgcgacga
     8161 cggcatcatc gacatgaacg acgtggcgcg cgtgctcaag tacaagcacg aggcgctcgg
     8221 ccagggcggc gtgctgcagc tggaagtgga ttgcggcaac ttcgcgcagg tggccgggct
     8281 ggccaacctc aagcactggc tggaactgcg caaggccgtg ttcctggaag acggcagcga
     8341 cggcgggctc gatccgccgc gcggactgct gctgctgggc gtgcagggct ccggcaagag
     8401 catggcggcc aaggcggtgg ccggcagctg gggcgtgccg ctgctgcgca tggatttcgg
     8461 cgcgctctac agcaagtgga gcggcgagac cgaaaaaaac ctgcgcgaca gcctcaaggt
     8521 ggccgacgcc atggagccgg cggtgctgtg gatcgacgag atcgagaaag gcctgtcggc
     8581 cgaggccggc gaagccgacg gcggcatgtc gcgccgcgtg ctcggcacgc tgctcacctg
     8641 gatgaacgag cgcaagagcc gcgtgttcct cgccgccact gccaacgaca tcgccacgct
     8701 gccgccggag ctgatgcgca agggccgctt cgatgaaatc tttttcgtcg acctgcccga
     8761 cgacgccacg cgcgcggaaa tcttccggcg gcaactggcg cggcgcaaac aggcgccaga
     8821 agggttcgac ctgcgcgcgc tggcggcaaa gtccgtcggc ttctccggcg cggagatcga
     8881 gcaggcggtg gtgtccgcgc tgtacgcggc caaggcgtcg caccagccga tgagcagcgc
     8941 gctggtcgcc gaggagctgg cgcgcacgcg gccactgtcg gtggtgatgg cggaaaaaat
     9001 cgccgcgctg cgcgcctggg ccagctcgcg cacggtgccg gcgaactgat tttttgctcc
     9061 accgcactgc ccttgatcat gttattattt gttgtacgtt tttatttttg aacaatattt
     9121 aataacatca cgatgagcct tgcggtggat gcaaaaaccg gcgccggcga tctgctggcg
     9181 ccgctgaaaa aacatttcgg cttcgacgcc ttccgccccg gccaggaagc ggtggtgcgc
     9241 gacgcgctgg ccggccgcga cctgctcgcg gtgatgccca ccggcggcgg caagtcgctg
     9301 tgcttccagc tgccggcact gctcaccgat ggcgtgatga tcgtggtctc gccgctcatc
     9361 gcgctgatgc aggaccaggt
//
LOCUS       DASZZC010000020         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_964289,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000020 DASZZC010000000
VERSION     DASZZC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964289"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VHE37_00520"
     CDS             complement(<1..189)
                     /locus_tag="VHE37_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34036.1"
                     /translation="MLLRHVAHKVMINFLSWSVRHIEGARRAADKGRMNIQSSRIARA
                     IAALLLLLPLASPAMVYLP"
     gene            complement(393..896)
                     /locus_tag="VHE37_00525"
     CDS             complement(393..896)
                     /locus_tag="VHE37_00525"
                     /inference="COORDINATES: protein motif:HMM:NF022605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HVT34037.1"
                     /translation="MVVRFIGGLAERVAAVALALTLAQFPVYYDAYLNTVAGARAEAE
                     ARLHELEREAAGVQMSVEAFIAHHEASADPAFAASGRMHRTTLEHYRHYSGLEHAMSA
                     APVWQKPLVLAQNFEPALHQATHFTPAVPLTPEALIYAALGLLLAWLTASLLARPFRR
                     RTTQTGS"
     gene            complement(906..1409)
                     /locus_tag="VHE37_00530"
     CDS             complement(906..1409)
                     /locus_tag="VHE37_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34038.1"
                     /translation="MNAQIRAGFLLLLLCGATAHAGEDEGDAPKYQALKARSAAPKDA
                     DINPAVTLGALLSQSGEKDWSTDRAARIEGYVIQVELEPDGDVHLVLAGAAHEPDTRK
                     WVVTEVPKAVRDRSKSLSVSALRKLVGRQVHVTGWMYWEPDDDSQDPRGTRWELHPVT
                     SVMPTGS"
     gene            complement(1477..>2732)
                     /locus_tag="VHE37_00535"
     CDS             complement(1477..>2732)
                     /locus_tag="VHE37_00535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT34039.1"
                     /translation="IADVEPLRLQRRQAALIFADMTGFSRLRDDELPAYWNQLMPELA
                     RVLSRYAHHVLLQSTWGDALYIVVDAAQPAAAAALDIVGAVQRMRGSLPGALTSLNLR
                     LSIHYAPVYTGWDPVGQGKLYYGSHVSLAARIEPVTPPGSIYVTEALAAQLTLEGAQQ
                     FDLSYAGEVELAKRFGKRRMFNLRAAAARRVPAGALVSPAEATPSESPAHLRGLVIGC
                     SGALLLSDEQKDWVRRKLAERVLPALREHFSPTRTTLVMGLAPGADLLFAESVQEWLS
                     ASGIAQVVRSVLPVPPEAILADWNDKVAASGAPDIEAETRASWAAMRRVLGQSLSVVN
                     LWPPEADVVDLATKSYRQRQYRRLGAYLVRHCDVLVAIYNRSAVGGAGGTAEVVKWWR
                     EAASGGATRALAVLDPVKREVTLEL"
BASE COUNT          404 a          975 c          930 g          423 t
ORIGIN      
        1 cggcaggtag accatcgccg gcgaggccag cggcagcagc aggagcagcg cggcgatggc
       61 gcgggcaata cgggacgatt gaatgttcat gcggcccttg tcggccgcgc ggcgcgcgcc
      121 ttcaatatga cgcacagacc acgagagaaa attaatcatg actttgtgcg cgacatgtcg
      181 gagcagcatt gcttctgccc gccagtaacg gcagcgccgc gcaggtctgg ttgatctaaa
      241 tcaatcaggt aggtggcctg cgaaccgaac cggctgtcgg ttgcggacgc gaacggcgca
      301 agtcagccgc ccggcggtgc gaaggttttg tctacggatt cctacgcgcc atttccgctt
      361 cgcggattgc gcgatgctga caacgcgggg cgttaactgc cggtctgtgt cgtcctgcgc
      421 cggaagggcc gggccagcag cgatgccgtc agccacgcca gcagcaggcc gagcgccgca
      481 tagatcagcg cttcaggcgt gagcggcacg gcaggcgtga aatgcgtcgc ctggtgcagt
      541 gccggctcga agttttgcgc gagcaccagc ggcttctgcc ataccggagc cgcgctcatc
      601 gcgtgctcga gcccgctgta gtggcggtaa tgctcgagcg tggtgcggtg catgcggccg
      661 gaggcggcga aggccggatc ggcgctggcc tcgtggtggg cgatgaaagc ctccacgctc
      721 atctgcacgc ccgccgcttc gcgctccagc tcgtgcagcc gcgcctcggc ctcggcgcgc
      781 gcgccggcca ccgtgttgag ataggcgtcg tagtacaccg ggaactgcgc cagcgtcagc
      841 gccagcgcga cggcggcgac gcgctcggcc aggccgccga tgaaacgcac aaccatgtgc
      901 ggacgtcagc tgccggtcgg catgaccgag gtgaccgggt gcagttccca gcgcgtgccg
      961 cgcgggtcct ggctgtcgtc gtccggctcc cagtacatcc agccggtgac gtgcacctgc
     1021 ctgcccacca gcttgcgcag cgcgctcacc gacagcgact tgctgcggtc acgcacggcc
     1081 ttcggcactt cggtgaccac ccacttgcgc gtgtcgggct cgtgcgccgc gcccgcgagc
     1141 accaggtgca cgtcgccgtc cggttccagt tccacctgga tgacgtagcc ctcgatgcgc
     1201 gccgccctgt cggtgctcca gtccttctcg cccgattgcg acagcagcgc gccgagcgtg
     1261 actgccggat tgatgtcggc gtccttcggc gccgcgctgc gcgccttgag tgcctggtac
     1321 ttgggcgcgt cgccctcgtc ttcgccggca tgtgccgttg cgccgcacag caacagcagc
     1381 aaaaagcccg cacggatttg cgcgttcatc ggcagcctcc tccttccgga tgcggccgaa
     1441 tctacgcccg ccgcgtgcgg cggacaaccg cggcattcac agctccagcg tgacctcgcg
     1501 cttgaccggg tcgagcaccg ccagcgcgcg cgtcgcaccg ccgctcgcgg cttcgcgcca
     1561 ccacttcacc acctcggcag tgccgcccgc gccgcccacc gcactgcggt tgtagatggc
     1621 caccagcacg tcgcaatggc gcaccaggta ggcgccgagc cggcggtact gccgctggcg
     1681 gtaggacttg gtggcaaggt cgaccacatc ggcttccggc ggccacaggt tgaccaccga
     1741 caggctctgg ccgagcacgc ggcgcatcgc tgcccagctc gcgcgggttt ccgcttcgat
     1801 atccggcgcg ccgctggcgg cgaccttgtc attccagtcg gccaggatcg cttccggcgg
     1861 caccggcagc acgctgcgca ccacctgcgc gatgcccgac gcagagagcc attcctgcac
     1921 gctctcggcg aacagcaggt cggcacccgg cgccagtccc atcaccagcg tggtgcgcgt
     1981 ggggctgaaa tgttcgcgca gcgccggcag cacgcgctcg gccagcttgc gccgcaccca
     2041 gtccttctgc tcatcgctga gcagcagcgc gccgctgcag ccgatgacca gtccgcgcag
     2101 gtgcgcgggc gactccgacg gcgtcgcctc ggccgggctg accagcgcgc ccgccggcac
     2161 ccgccgcgcc gccgcggcgc gcaggttgaa catgcgccgc ttgccgaaac gcttggccag
     2221 ctccacttcg ccggcgtagc tgagatcgaa ctgctgcgcg ccttccagcg tgagctgtgc
     2281 ggccagtgcc tccgtcacgt agatgctgcc gggcggcgtc accggctcga tgcgcgccgc
     2341 cagcgacacg tggctgccgt agtacagctt gccctggccc accgggtccc agccggtgta
     2401 gaccggcgcg tagtgaatcg acaggcgcag gttgagcgag gtgagtgcgc cgggcagcga
     2461 gccgcgcatg cgctgcaccg cgccgacgat gtccagcgcg gccgccgccg ctggttgcgc
     2521 ggcgtcgacc acgatataca gcgcatcgcc ccaggtgctc tgcagcagca cgtggtgcgc
     2581 gtagcgcgac agcacgcgcg ccagctccgg catcagctgg ttccagtagg ccggcagttc
     2641 gtcgtcgcgc aggcgcgaga agccggtcat gtcggcgaag atcagcgcgg cctgacgccg
     2701 ctgcaggcgc agcggttcga cgtcggcgat gg
//
LOCUS       DASZZC010000021         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1551,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000021 DASZZC010000000
VERSION     DASZZC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="purH"
                     /locus_tag="VHE37_00540"
     CDS             complement(<1..635)
                     /gene="purH"
                     /locus_tag="VHE37_00540"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183185.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HVT34040.1"
                     /translation="MKTPVRRALISVSDKNGIVDFARELARRGVAILSTGGTYKLLDE
                     SGVEATEISEHTGFPEIMDGRVKTLHPKVHGGLLGRRGTDDAVMREHGIAPIDLLCVN
                     LYPFEATVARADSTYEDAVENIDIGGPAMLRAAAKNHAYVTVLTDPADYAGVLAEMDA
                     GGVTQETRNRLALKTYAHTSRYDAAVAGYLSSHLGHGKTTTFPGVLGLQFE"
     gene            complement(718..981)
                     /locus_tag="VHE37_00545"
     CDS             complement(718..981)
                     /locus_tag="VHE37_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT34041.1"
                     /translation="MSVHKSDIKPLCHSVSDCLDDYFKTLNGHAPKNLYEMILEQVEP
                     PLLRATLSYCAGNQSKAADMLGLNRATLRKKIKLHKIKPQAAP"
     gene            complement(1094..2416)
                     /gene="prmA"
                     /locus_tag="VHE37_00550"
     CDS             complement(1094..2416)
                     /gene="prmA"
                     /locus_tag="VHE37_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HVT34042.1"
                     /translation="MAWLQLMVRSAHPEFADEILQAQGAQAVSFIDAQDDPVLEPAPG
                     ATPLWSHTLTLGLFAEHTDLAPVLATLRELLPDGSQAQFTHELIEDQDWVRVWLKDCP
                     PLKFGERLWVVPREKRAEVTQADAIILELDPGLAFGTGTHPTTALCLDWLARQDLAGK
                     SLLDYGCGSGILAIAALKLGAQRAVAVDIDPQALIAARDNAAVNGVEGALTTLASDDF
                     IPFAADVVVANILANPLIALAPLLASSSRPGGALALAGLLDRQAEEVRAAYEPWFEFQ
                     PDRSKDGWTLLSAQCEAAAIVNYLEISPALGTGGQPRPEHFAQLKRDGYDAVINLSVA
                     TSPNALPDEGALCAQQGLHYTHIPVEWDKPARADLEKFFSTMRTHHDRRVFVHCVMNK
                     RVSAFVYLHRVLNQGADAHAARHQLHRIWQPNAVWQRYIDDMLRTSAE"
     gene            complement(2417..3763)
                     /gene="xseA"
                     /locus_tag="VHE37_00555"
     CDS             complement(2417..3763)
                     /gene="xseA"
                     /locus_tag="VHE37_00555"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HVT34043.1"
                     /translation="MPSASSPARTIYQVNELAELLREIVEGSLPRVWVQGEISNLSRP
                     ASGHWYFTLKDARAQLRCAMFRQHNFHVRPLPRDGDSVLVRGQVSVYTARGDLQLICE
                     HLEPAGEGALLRAFEELKKKLAAEGLFDEHLKRPIPAVPRGIGLITSGTGAALHDVRT
                     TLERRFPLGTVYLLPVPVQGVEAAPAIVRALDALPARAPVDVIILARGGGSLEDLWSF
                     NDERVARAIRACTVPVVTGVGHEIDTTIADFAADLRAATPTAAAELVSPDADEWRQRI
                     ADAQAELGNGIERRLEKQRETLQVKAGRLALLHPGRRMSDATQRLDELAERLHHAGGA
                     TLRRTQEFLRLSGERLLRCAPQASIELKRDRLAQLQQRLQERLMARTADMQARHRHLH
                     ELLRSLNPRAVLERGYAIALAADGRVLTDAASVAAGDAITVQLARGTLETSVTGKK"
     gene            3817..5280
                     /gene="guaB"
                     /locus_tag="VHE37_00560"
     CDS             3817..5280
                     /gene="guaB"
                     /locus_tag="VHE37_00560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HVT34044.1"
                     /translation="MRIELEALTFDDVLLQPAYSEVLPRQVDTSTTLTRGLRLNIPLL
                     SAAMDTVTEGRLAIALAQEGGIGIIHKNMTAERQAAEVRAVKKYESGVITDPIVVPPD
                     MTVGEVLKLTRANRISGVPVVDGKELVGIVTSRDLRFETRYDEPVSVVMTRKDKLVTV
                     LEGAAKDEIIGKLHRHRIEKVLVVNGKFQLRGMITVKDIQKSSDYPLACKDSHARLRV
                     GAAVGTGPDTDERVRALVAAGVDVVVVDTAHGHSKGVLDRVRAIKKQFPDLQVIGGNI
                     ATPEAALALVDAGADGVKVGIGPGSICTTRIVAGVGVPQISAVMQVAEALKKTGVPLI
                     SDGGVRYSGDFAKALAAGAHAVMVGSMLAGTEEAPGEVELYQGRSYKSYRGMGSMGAM
                     AQGSKDRYFQDTTAEVEKLVPEGIEGRVPYKGPMSAIVHQMVGGLRACMGYTGCQSIE
                     ALRTNTRFVKITGAGIKESHVHDVTITKEAPNYRVDS"
     gene            5277..>6037
                     /gene="guaA"
                     /locus_tag="VHE37_00565"
     CDS             5277..>6037
                     /gene="guaA"
                     /locus_tag="VHE37_00565"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HVT34045.1"
                     /translation="MSNIHADKILILDFGSQYTQLIARRVRELGVYCELHPWDMDEAA
                     IRSFAPRGIILSGGPESTLDKNSPRAPQAVWDLGVPVLGICYGLQTMAMQLGGKVEAS
                     NTREYGYAQVRARGHSALLKDIEDRLSPQGHGLLDVWMSHGDRVTKIPRGFKLIASTA
                     DVPIAGMADEARKLYAIQFHPEVTHTTQGKRIYARFVHEICGCGNAWTPGNIIDDAVA
                     RVRQQVGRNKVLLGLSGGVDSSVVAALLHKAIGKQ"
BASE COUNT          980 a         1957 c         2085 g         1015 t
ORIGIN      
        1 ttctcgaact gcaggccgag cacgccggga aaggtggtgg tcttgccgtg gccgagatgg
       61 ctcgacaggt agccggccac cgccgcgtcg tagcgcgagg tgtgcgcgta ggttttcagc
      121 gcaagccggt tgcgcgtctc ctgcgtcacg ccgcccgcgt ccatctccgc cagcacgccg
      181 gcgtagtcgg ccggatcggt cagcacggtg acgtaggcgt ggttcttggc cgccgcgcgc
      241 agcatggccg ggccgccgat gtcgatgttc tccaccgcgt cttcgtaggt ggagtccgcc
      301 ctggccacgg tggcctcgaa cgggtagagg ttgacgcaca gcaggtcgat gggggcgatg
      361 ccgtgttcgc gcatcaccgc gtcgtcggtg ccgcgccgcc cgagcaggcc gccgtgcacc
      421 ttggggtgca gcgtcttgac gcggccgtcc atgatctcgg gaaagccggt gtgctcgctg
      481 atctcggtgg cttccacgcc cgattcgtcc agcagcttgt aggtgccgcc ggtggacagg
      541 atggcgacgc cgcggcgcgc cagctcgcgg gcgaagtcga cgatgccgtt cttgtcggag
      601 accgagatca gcgcgcggcg tacgggtgtt ttcatggcag gcgtctggtt gtccagctgt
      661 ataaatgaaa aggccgttcg tgcatgcgcg acgaacggcc gggtgatcgg tggcgtttca
      721 gggggctgct tgcggcttga tcttgtgcag cttgatcttc ttgcgcaggg tggcgcggtt
      781 gaggccgagc atgtcggcgg ccttgctctg gttgccagcg caatacgaca gcgtggcgcg
      841 cagcagcggc ggctcgacct gctcgaggat catctcgtac aggtttttcg gcgcgtgtcc
      901 gttcagcgtc ttgaagtagt cgtcaagaca atccgagacg gaatgacaca gcggcttgat
      961 atcggatttg tgtacggaca ttttcggctt cccctcgccc tgcgatgcgg tgccgcaggc
     1021 ccgtctcgaa aacgggcagc aatcatactc tcaacgcccg ccgaaaaaaa aggatttgct
     1081 cagacgaaaa aatttactca gcgcttgtgc gcagcatgtc atcgatatag cgctgccaca
     1141 ccgcgttcgg ctgccagatg cggtgcagct ggtgccgcgc ggcgtgcgca tctgcacctt
     1201 gattgagcac gcggtgcagg tagacgaagg ccgacacgcg cttgttcatg acgcaatgga
     1261 cgaacacgcg ccggtcatgg tgcgtgcgca tcgtgctgaa aaatttttcc aggtcggcgc
     1321 gtgcgggttt gtcccactcc accggaatat gcgtgtaatg caaaccctgc tgcgcgcaca
     1381 gtgcgccttc atcaggcagc gcgttgggcg aagtcgccac ggaaaggttg atcacggcgt
     1441 cgtagccgtc gcgcttgagc tgcgcaaaat gttccggccg cggctgtccg ccggtaccga
     1501 gcgcgggcga gatctccagg taattgacga tcgccgccgc ctcgcactgc gcgctgagca
     1561 gagtccagcc gtccttgctg cggtcgggct gaaactcgaa ccacggttcg taggccgcac
     1621 gcacttcctc cgcctgccgg tcgagcaggc cggccagcgc cagcgcaccg ccgggccggc
     1681 tgctggaggc cagcagcggc gccagtgcga tcagcggatt ggccaggatg ttggccacga
     1741 ccacgtcggc cgcaaaggga atgaagtcat cgctggccag cgtcgtcagc gcgccttcca
     1801 cgccgttgac cgccgcgttg tcgcgcgcgg cgatcagcgc ctgcggatcg atatccaccg
     1861 ccaccgcccg ctgcgcaccg agtttgagcg cggcgatggc caggatgccg gagccgcagc
     1921 catagtcgag caggctttta ccggcgaggt cctgccgtgc caaccagtcg aggcacagcg
     1981 cggtggttgg atgcgtgccg gtgccgaagg ccagtccagg atcgagctcg aggatgatgg
     2041 cgtcggcctg cgtcacctcg gcgcgctttt ccctgggcac cacccacagg cgctcgccga
     2101 atttcaacgg cgggcagtcc ttgagccaga cgcgcaccca gtcctggtcc tcgatcagtt
     2161 catgtgtgaa ctgcgcctgc gatccgtccg gcagcagctc gcgcagcgtg gcaagcaccg
     2221 gcgccaggtc ggtgtgctcg gcaaacaggc cgagcgtgag cgtgtggctc cacagcggcg
     2281 tggcgccggg cgccggctcg agtaccgggt cgtcctgcgc gtcgatgaag ctgactgcct
     2341 gcgcgccttg cgcctgcagg atttcgtcgg cgaattccgg gtgcgcgctg cgcaccatga
     2401 gctgcagcca ggccatttat ttttttccgg tcaccgaggt ttcgagcgtg ccccgcgcca
     2461 gctgcacggt gatggcatcg cccgcggcca cgctggcggc atcggtcagc acgcggccgt
     2521 cggccgccag tgcgatggcg tagccgcgct cgagcacggc gcgcggattg aggctgcgca
     2581 gcagctcgtg cagatgccgg tggcgcgcct gcatgtcggc ggtgcgtgcc atcagcctct
     2641 cctgcagtcg ttgttgcagc tgcgccagcc ggtcgcgctt cagttcgatg gatgcctgcg
     2701 gtgcgcagcg cagcaggcgt tcgccggaca ggcgcaggaa ttcctgcgtg cggcgaagcg
     2761 tggcgccgcc ggcgtggtgc aggcgttcgg ccagctcgtc caggcgctgc gtggcgtcgc
     2821 tcatccttcg tcccgggtgc agcagggcca gccgcccggc tttcacctgc agggtctccc
     2881 gctgtttctc caggcgccgt tcaatgccgt ttccgagttc ggcctgcgca tcggcgatgc
     2941 gctgccgcca ctcgtccgca tccgggctga ccagctccgc tgccgcggtc ggcgtggcgg
     3001 cgcgcaggtc ggcggcgaag tcggcgatgg tggtgtcgat ctcgtggccc acgccggtca
     3061 ccaccggcac cgtgcaggcg cggatggcgc gcgccacccg ctcgtcgttg aatgaccaca
     3121 ggtcctcgag cgagccgccg ccgcgcgcca ggatgatgac gtcgaccggc gcgcgcgcgg
     3181 gcagcgcgtc gagcgcgcgc acgatggccg gcgcggcttc cacgccttgt accggcaccg
     3241 gcaataaata gacggtgccg agcgggaagc ggcgttccag cgtggtgcgc acgtcgtgca
     3301 acgccgcgcc ggtgccggag gtgatcaaac cgatgccgcg tggtacagcg gggatcggcc
     3361 gcttgaggtg ttcgtcgaac aggccttcgg ccgccagctt cttcttcagt tcctcgaacg
     3421 cgcgcagcag tgcgccttcg ccggccggtt ccaggtgctc gcagatcagc tgcaggtcgc
     3481 cgcgcgcggt gtacacgctc acctggccgc gcaccagcac gctgtcgccg tcgcgcggca
     3541 gcgggcgcac gtggaaattg tgctggcgga acatggcgca gcgcagctgt gcgcgcgcgt
     3601 ccttcagggt gaagtaccag tggccggatg ccgggcgcga caggttggag atttcgccct
     3661 gcacccatac gcgcggcagg ctgccttcga cgatctcgcg cagcagttcg gccagttcat
     3721 tgacctggta gatggtgcgg gcaggggagg aggcggaggg cattacgtgc ggagcgaaag
     3781 gcgcatccgt ataatattgc tttgccggag cctgaaatga gaattgagct agaagcgctc
     3841 actttcgacg acgtacttct ccagcccgcc tactccgaag tactgccgcg acaggtcgac
     3901 acctcgacca ccctgacccg cggcctcagg ctgaacatcc cgctgctgtc ggccgccatg
     3961 gacacggtga ccgaggggcg cctggccatc gcgctggcgc aggaaggcgg catcggcatc
     4021 atccacaaga acatgaccgc cgagcgccag gcggccgaag tgcgtgcggt gaagaaatac
     4081 gagagcggcg tcatcaccga cccgatcgtc gtgccacccg acatgacggt gggcgaggtg
     4141 ctcaagctca cgcgcgccaa ccgcatctcc ggcgtgccgg tggtcgacgg caaggaactg
     4201 gtcggcatcg tcaccagccg cgacctgcgc ttcgagacgc gctacgacga gccggtgtcc
     4261 gtggtgatga cgcgcaagga caagctggtc accgtgctgg aaggcgcggc caaggacgag
     4321 atcattggca agctgcaccg gcaccgcatc gagaaggtgc tggtggtcaa cggcaagttc
     4381 cagctgcgcg gcatgatcac ggtgaaggac atccagaagt cgagcgatta cccgctcgcc
     4441 tgcaaggaca gccatgcgcg cctgcgcgtg ggcgccgctg tcggcaccgg cccggatacc
     4501 gatgagcgcg tgcgtgcgct ggtcgctgcc ggcgtcgacg tggtggtggt tgacaccgcg
     4561 catggtcaca gcaagggcgt gctcgaccgc gtgcgcgcca tcaagaagca gttccccgac
     4621 ctgcaggtga tcggcggcaa catcgccacc cccgaggctg cgctcgcgct ggtcgatgcc
     4681 ggcgccgacg gcgtcaaggt cggcatcggc cccggctcca tctgcaccac gcgtatcgtt
     4741 gccggcgtgg gcgtgccgca gatcagcgcg gtgatgcagg tggcggaggc gctgaagaaa
     4801 accggcgtgc cgctgatatc cgacggcggc gtgcgctact ccggcgactt cgccaaggcg
     4861 ctggccgccg gcgcgcatgc ggtgatggtc ggcagcatgc tggccggcac cgaggaagcg
     4921 ccgggcgaag tcgagctgta ccagggccgc tcgtacaaga gctaccgtgg catgggctcg
     4981 atgggtgcca tggcgcaggg ctccaaggac cgctacttcc aggacaccac cgcggaagtg
     5041 gagaagctgg tgccggaagg catcgagggc cgcgttcctt ataaaggacc gatgtcggcg
     5101 atcgttcacc agatggtcgg cgggctgcgc gcgtgcatgg gctacaccgg ctgccagagc
     5161 atcgaggcgc tgcgcaccaa cacgcgcttc gtcaagatca ccggcgccgg catcaaggaa
     5221 agccatgtgc acgacgtgac catcaccaag gaagcgccga actaccgggt tgattcgtga
     5281 gcaacatcca cgccgacaag atcctcatcc tggatttcgg ttcgcagtac acccagctga
     5341 tcgcgcgccg cgtgcgcgag ctgggcgtct attgcgagct gcacccatgg gacatggacg
     5401 aagccgccat tcgcagcttc gcgccgcgcg gcatcatcct ctctggcggg ccggaatcca
     5461 cgctggacaa gaattcgccg cgcgcgccgc aggcggtgtg ggacctgggc gtgccggtgc
     5521 tgggcatctg ctacggcctg cagaccatgg ccatgcagct gggcggcaag gtcgaagcgt
     5581 cgaacacacg cgagtacggc tacgcccagg tgcgcgcgcg cgggcacagt gcgctgttga
     5641 aagacatcga ggaccgcctc tcgccgcagg gccacggcct gctcgatgtg tggatgagcc
     5701 acggcgaccg cgtgacgaag attccacgcg gcttcaagct gatcgcctcc accgccgacg
     5761 tgcccatcgc cggcatggcc gacgaggcgc gcaagctcta cgccatccag ttccacccgg
     5821 aagtcacgca caccacgcag ggcaagcgca tctacgcgcg cttcgtgcac gagatctgcg
     5881 gctgcggcaa cgcctggacg ccgggcaaca tcattgacga tgctgtcgcg cgcgtgcgtc
     5941 agcaggtcgg caggaacaag gtgctgctcg gtctctccgg cggagtcgat tcatcggtgg
     6001 tcgcggcact gctgcacaag gccatcggca agcagtt
//
LOCUS       DASZZC010000022         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1222094,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000022 DASZZC010000000
VERSION     DASZZC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222094"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VHE37_00570"
     CDS             complement(<1..1459)
                     /locus_tag="VHE37_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVT34046.1"
                     /translation="MSQPQRFGKFEVRRRLGSGGQSAVYLAHDPDLRRDVAIKLLKPG
                     AAGAEAVRREARTVSQLRHRNIVPIHEMGEHQNRIYLVFEYVEGRTLQQALAQDGAIA
                     PRRAAEMLNSILDALAEAHDKGVIHRDLKPSNILLDGAGEPHVMDFGIASAPTRESGL
                     EGDLYGSPAYMAPEYVKSRSISEQYDVFSAGLILYEMVCGRRAVQGDNPYQALHQIAN
                     MPLTFPAGAVEAIGPVLHDIIVKATAKEPELRYASVKQMHDALDAYLNPAADAAVAQA
                     AKASTLEFLLRRMKVKGDFPAMSSAMSAIQRIAASNHSNVSSLASAILKDYALTNKIL
                     RLVNSAYYANRSRERIRTVTRAIVMMGFDAVRSIAISLMLFDRIRDKNHAGALKEEFL
                     RANLAGILARELCARINPSQSEEAFICALFHNLGRLLTCYYFWEESEAIRALVAQGTG
                     EDAAAVRVLGIGYTELGMGVAESWGFPASITQSMLG"
     gene            1644..>2311
                     /locus_tag="VHE37_00575"
     CDS             1644..>2311
                     /locus_tag="VHE37_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HVT34047.1"
                     /translation="MSYPQGRWICTSWLGFAAVVAALALTPAQASAPSSVHDFTYDHG
                     TYATSLIDGGDGSFYGTTYQGGARGYGTVFKLTHGVLTTLHSFNRTDGANPLGALLPA
                     SDGKLYGTTGGGGAQDFGTVFVITRDGTFNHLYDFDGTHGYLPTARLIQDSDGSFYGT
                     TQGGGTAAGGNGTVFRITGNGAITTLHSFSGGDGFQPESGLTQTADGTFYGTTYAGGT
                     GGAGT"
BASE COUNT          377 a          778 c          751 g          405 t
ORIGIN      
        1 agccgagcat gctctgcgtg atcgacgccg ggaaacccca gctctccgcc acgcccatgc
       61 ccagctcggt gtagccgatg ccgagcacgc gcaccgccgc ggcgtcttcg ccggtgccct
      121 gcgccaccag cgcgcggatc gcctccgatt cttcccagaa gtaatagcag gtgagcagcc
      181 ggcccaggtt gtggaacagc gcgcagatga aggcttcttc cgattgcgac gggttgatgc
      241 gcgcgcacag ttcgcgcgcc aggatgccgg cgagattggc gcgcaggaat tcctccttga
      301 gcgcgcccgc atggttcttg tcgcgtatgc ggtcgaacag catgagcgag atggcgatgc
      361 tgcgcaccgc gtcgaaaccc atcatcacga tggcgcgcgt caccgtgcgg atgcgctcac
      421 gcgaacggtt ggcgtagtac gccgagttga ccaggcgcag gatcttgttg gtcagcgcgt
      481 agtccttgag gatggcgctg gccaggctgc tcacgttcga gtggttcgat gccgcgatgc
      541 gctggatggc gctcatggcc gacgacatgg ccgggaaatc acccttcacc ttcatccgcc
      601 gcagcaggaa ttccagcgtg ctggccttgg ccgcctgcgc caccgcggca tcggccgccg
      661 gattcaggta cgcgtcgagc gcatcgtgca tctgcttcac gctggcatag cgcagctccg
      721 gctccttggc ggtggccttg acgatgatgt cgtgcagcac cgggccgatc gcctccaccg
      781 cgccggccgg gaaggtcagc ggcatgttgg cgatctggtg cagcgcctgg tacgggttgt
      841 cgccctgcac cgcgcggcgg ccacacacca tctcgtacag gatcaggccg gccgagaaca
      901 catcgtattg ctcgctgatg ctgcggctct tcacgtactc cggcgccatg taagccgggc
      961 tgccgtagag atcgccctcc aggccggact cgcgcgtggg cgcgctggcg atgccgaaat
     1021 ccatcacgtg cggttcgccg gcgccgtcga gcaggatgtt cgatggcttg agatcgcgat
     1081 ggatcacgcc cttgtcatgc gcctcggcca gcgcatcgag aatgctgttg agcatctccg
     1141 ccgcgcgccg cggtgcaatc gcgccatcct gggccagcgc ctgctgcagc gtgcggcctt
     1201 ccacgtattc gaagaccagg tagatgcggt tctggtgctc gcccatctcg tggatcggca
     1261 cgatgttgcg atgacgcagc tggctgacgg tgcgcgcctc gcggcgcacg gcctcggccc
     1321 cggccgctcc gggcttgagc agcttgatgg cgacatcccg gcgcaggtcc gggtcatgcg
     1381 ccagataaac ggcgctctgg ccgccgctgc caaggcggcg gcgcacctcg aacttgccga
     1441 agcgctgggg ttgcgacacg cagacgaccg gaagggacaa tttgttgctg acagtgcaag
     1501 ttcaaggcct tgccgcccct gccgtcgcca cgcccgcccg gcaggaagaa atttcctact
     1561 cgccggttga taactatgtc atcgatccga atcgcggccg gcgtagactt tgtccccctc
     1621 aatcgacgtc aagggagccg atcatgtcgt atccgcaggg cagatggatc tgcacatcgt
     1681 ggctcggctt cgccgcggtg gttgcggccc tcgcactgac gccggcgcag gcttcggccc
     1741 cgtcctccgt ccatgatttc acctacgacc atggcacata cgccaccagc ctgatcgatg
     1801 gcggggatgg cagcttctac ggcacgacct accagggcgg cgcgcgcggt tatggcacgg
     1861 tgttcaagct cacgcacggc gtgctgacca cgttgcattc gttcaaccgg actgacggcg
     1921 ccaacccgct gggagcgctg ttgccagcca gcgacggcaa gctctacggc accaccggcg
     1981 gcggcggtgc gcaggatttc ggcacggtgt ttgtcatcac ccgcgacggc accttcaacc
     2041 acctctatga cttcgacggc acgcacgggt atctgccgac ggcgcgcctg atccaggaca
     2101 gcgacggcag tttctacggc accacgcagg gcggcggcac cgctgccggc ggcaatggca
     2161 cggtgttccg catcaccggc aacggtgcaa tcaccacgct gcattcgttc agcggcggcg
     2221 acggcttcca gccggaaagt gggttgacac aaaccgcgga cgggactttc tacggcacca
     2281 cctacgctgg cggcaccggc ggcgccggga c
//
LOCUS       DASZZC010000023         5888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_708388,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000023 DASZZC010000000
VERSION     DASZZC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708388"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VHE37_00580"
     CDS             complement(<1..581)
                     /locus_tag="VHE37_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HVT34048.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGAPKVIENSEGERTTPSIVAYTD
                     DGQTIVGRSAKRQAITNPNSTLYAIKRLIGRRYEDQVVQKDIAMVPYKIVKAANGDAW
                     VEVKGNKMAAQQISAEVLIKMKKTAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKEGADQTVLVF"
     gene            complement(641..1186)
                     /gene="grpE"
                     /locus_tag="VHE37_00585"
     CDS             complement(641..1186)
                     /gene="grpE"
                     /locus_tag="VHE37_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HVT34049.1"
                     /translation="MTDAAADSAPNPGSPADPVTELAALRAELEQVRAAAAQAQDARL
                     RIAAESENVRRRLERDAQNDRKYANEKLLGDLLGIADSLELGLKAAGSPDAQAKAIAD
                     GMQLTYRQLMAALEKNGVKQLDPKGEAFNPDAHQAMSMVESAEVKPNHVVEVMQKGYK
                     LHERLLRPAMVVVAKAPAAAS"
     gene            complement(1277..2323)
                     /gene="hrcA"
                     /locus_tag="VHE37_00590"
     CDS             complement(1277..2323)
                     /gene="hrcA"
                     /locus_tag="VHE37_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HVT34050.1"
                     /translation="MSNQSPLDARAGELLKLLVERYIHDGQPIGSRTLSRLASLGLSA
                     ATIRNVMADLDHLGFVASPHTSAGRIPTQRGYRYFVDALLQPEPLTDVERGQIEHELL
                     PRDRGAADLVSAASAVLSQISAMAGVVTVPKRNRAILRRIEFLPLSDRRVLAILVVNQ
                     REVQNRVIATDRDYRAEELERYANALNEHFAGRDLVSLRNALLNEAAEAQARVNQVLV
                     EAASMAEQAMRDDAASPGYVVAGGSNLMAFNELADMQRLKNLFDALDRKRDLLNLFDQ
                     CVNADAMQIFIGEESGYRVLDECSVITAPYYVEGQVAGVLGVIGPTRMAYHRLIPLVS
                     ETARALTQGLKSET"
     gene            complement(2412..4799)
                     /locus_tag="VHE37_00595"
     CDS             complement(2412..4799)
                     /locus_tag="VHE37_00595"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVT34051.1"
                     /translation="MKKTNLRARGWLASLLLLSGMLGYASAHAAAGWVSTASKGLKIA
                     QGVAAQPVAAGRAMHIALALKPRNAAGLKALATAVNTPGNPAFGHFLTSAQFNQRYAP
                     DALQAQAVVEYLQQAGFTGVQLASNRMLVTASGTASQVQAAFNTTLRQFARDGRSWFV
                     NTRDAQVPAALGGVVAGVYGLNNVEHVLPARRQTALGLPNIDGTVLGLGLSPQELWAA
                     YNVGNTAAADQTTIAILAFGNMDPVIANLRSYEQTFGLPQVTVDVIPTGSPDHSTDQG
                     EDEWDMDTQTSTGMAGNVQKLVIYDGGDFQNNADHLDSMNRFVADNIAKAASYSVSSC
                     EVLQAVAGDMDATDAVLMQAAAQGQSFFTSTGDTGATCYDPYTGLVVGLTPLGSTNGV
                     PDSGIPAGQAYPASSPWAVGVGGTSLALDSTNARTAEISWNAGGGGPSLIEVAPQWQT
                     TEIVPSAVIGQRGTPDLAMDADSLVSGAAVFTTQYGELPSGGTSLSSPLALGAWARVQ
                     SASCNGYGLITPLIYQLAQTAGPATAVPGFYDIVAGTNGAFFANPGWDYNTGVGAFNV
                     AELITALPGTGACSGGASSTTMTATPASVSFGSETVHGTSSSQTVTLANTGISTLNIS
                     SVSVTGDYTVASNGCTAPLPAGGSCAIGLAFKPNAAGTRAGVMTVAAANAHSAMVHLT
                     GNGKNGPLLSLAPAALDFGTQPVMTASAPQHVTLKNIGTANLSIDSILVSSARSPDDF
                     SKTSTCGKTLLPHQQCTLSITFKPLLARPRSGNLRVVSSSENTVDQVTLTGTGTQ"
     gene            4947..5825
                     /locus_tag="VHE37_00600"
     CDS             4947..5825
                     /locus_tag="VHE37_00600"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HVT34052.1"
                     /translation="MSEFRTVGVIGKQLDPRVGPTLARLCEDLRALGCTLLVAPECAP
                     HVDVSADCIASLEQISQRANIAVVVGGDGTLLSAARALAPAGVPILGINQGRLGFMVD
                     VNPNEMKETLTQVFAGDYVREERLLLSARLRRDGAVSGPYLAVNDVVIRNQAAIRMLE
                     FETWMDGSFISQHRADGMIVCSPTGSTAYALSGGGPVMHPGLEAFALVPICPHTLSDR
                     PIVVGSRHLVRIVLGGAGGTSAMVTCDGQINETLNPGDAVEIERAPAALRLIHPRSYN
                     YFDILRSKLHWGRARA"
BASE COUNT          881 a         1934 c         2039 g         1034 t
ORIGIN      
        1 tcgaagacga ggacggtctg gtcggcaccc tccttgtcga ggccgtaggc cagtgcagcc
       61 gccgtcggct cgttgatgat gcggagcacc tcgagcccgg cgatcttgcc ggcgtccttg
      121 gtcgcctggc gctgcgaatc gttgaagtag gccggaacgg tgatgaccgc ttccgtgacc
      181 ttctcgccga gatagtcctc ggcggtcttc ttcatcttga tcagcacctc ggcgctgatc
      241 tgctgggccg ccatcttgtt gcccttgacc tcgacccagg cgtcgccatt ggccgccttg
      301 acgatcttgt acggcaccat ggcgatgtcc ttctggacga cctggtcctc gtagcggcgg
      361 ccaatcaggc gcttgatcgc gtacagcgtg ctgttggggt tggtgatggc ctggcgcttg
      421 gcgctgcggc cgacgatggt ctggccgtcg tcggtgtagg cgacgatgga cggcgtggtg
      481 cgctcgcctt cgctgttctc gatcaccttg ggtgcgccgc cttccatgat ggcgacgcag
      541 ctgttggtgg tgcccaggtc gatgccgatg atcttggcca tggtgtgtgc tctccgaaat
      601 tcctgaagtt gtcgcttatg tgggtgtgaa tcgggtcaat tcaagatgcg gcggccggcg
      661 ccttggcgac caccaccatt gccgggcgca gcaggcgctc gtgcagcttg tagcccttct
      721 gcatcacttc gaccacgtgg ttgggcttga cctccgcgga ttcgaccatg ctcatcgcct
      781 ggtgcgcgtc cgggttgaag gcctcgcctt tggggtcgag ctgcttgacg ccgttctttt
      841 ccagcgccgc catcagctgg cgataggtga gctgcatgcc gtcggcgatg gccttggcct
      901 gcgcatccgg cgaaccggcg gccttcagcc ccagctcgag gctgtcggcg atgccgagca
      961 ggtcgccgag cagtttctcg ttggcgtact tgcggtcgtt ctgtgcgtcg cgctccaggc
     1021 ggcgccggac gttctcgctc tcggcggcga tgcgcaggcg tgcgtcctgc gcctgcgccg
     1081 cggcggcacg cacctgctcc agctcggcgc gcagcgcggc cagttcggta accggatcgg
     1141 ccggcgagcc ggggttcgga gcgctatccg cagcagcatc agtcatgtta tgaaaaattg
     1201 atcagattaa agttaattca ataaataaaa acaaccgcga ccaaggtgcg gtcaagctgt
     1261 cgccgcaaga tgcggcctag gtttcgcttt tcaagccctg cgtcagggcg cgcgcggttt
     1321 ccgaaaccag cgggatcagg cggtgatagg ccatgcgcgt cgggccgatc acgcccagca
     1381 cgccggccac ctgtccttcc acgtaatacg gcgccgtgat cacgctgcat tcgtcgagca
     1441 cgcggtagcc ggattcctcg ccgatgaaga tctgcatggc atcggcattg acgcactggt
     1501 cgaacaggtt gagcaggtcg cgcttgcggt ccagcgcgtc gaacaggttc ttcagccgct
     1561 gcatgtcggc cagctcgttg aacgccatca ggttggagcc gccggccacc acgtaaccgg
     1621 gcgatgccgc atcgtcgcgc atggcctgct cggccatgct cgccgcctcc accaggacct
     1681 ggttgacgcg cgcctgcgcc tcggccgctt cgttgagcag cgcattgcgc aggctgacca
     1741 ggtcgcgacc ggcgaaatgc tcgttgagcg cgttggcgta gcgctccagt tcctcggcgc
     1801 ggtagtcgcg gtcggtggcg atgacgcggt tctgcacttc gcgctggttc accaccagga
     1861 tggccagcac gcggcggtcc gacagcggca ggaactcgat gcgccgcagg atcgcgcggt
     1921 tgcgcttggg cacggtgacc acgccggcca tggcggaaat ctgcgacagc accgccgagg
     1981 ctgcggagac caggtcggcc gcgccgcggt cgcgcggcag cagctcatgc tcgatctggc
     2041 cgcgctcgac gtcggtcagc ggttccggct gcagcagcgc gtcgacgaag tagcggtagc
     2101 cgcgctgcgt cgggatgcgc ccggccgagg tgtgcggcga agcgacgaag cccaggtggt
     2161 ccaggtcggc catcacgttg cggatggtgg ccgcggacag gcccagcgag gccaggcgcg
     2221 acagcgtgcg cgagccgatc ggctggccgt cgtggatgta gcgttcgacc agcagcttga
     2281 gcagctcgcc ggcgcgtgcg tcgagtggcg attggttgga catcgttgga aagaaaaaag
     2341 cccgctgcaa catgcagcgg gcctgatgga ctgtcaagac ggtccgcggg ttcccgcgga
     2401 accggctggc gtcattgcgt gccggtgccg gtgagcgtga cctggtcgac ggtgttctcg
     2461 ctgctgctga ccacgcgcag gttgccgctg cgcgggcgcg ccagcagcgg cttgaaggtg
     2521 atggacagcg tgcattgctg gtgcggaagc agcgtcttgc cgcaggtgct ggtcttggag
     2581 aaatcatccg gtgaccgggc cgacgagacc aggatcgagt cgatgctcag gttggcggtg
     2641 ccgatgttct tcagcgtgac gtgctgcggc gcgctggccg tcatcaccgg ctgcgtgccg
     2701 aaatccagcg ctgccggcgc cagcgacagc agcggcccgt tcttgccgtt gccggtgaga
     2761 tgcaccatcg ccgaatgcgc gttggccgcg gcaacggtca tcacgccggc gcgcgtaccc
     2821 gccgcgttcg gcttgaacgc caggccaatc gcgcagctgc cgccggccgg cagcggcgcg
     2881 gtgcagccat tgctcgccac cgtgtagtcc ccggtgaccg agaccgacga aatgttcagc
     2941 gtggaaatgc cggtgttggc cagcgtcacc gtctgcgacg acgaggtgcc gtgcaccgtc
     3001 tcgctgccga agctgaccga ggccggcgtg gccgtcatgg tggtcgacga agcgccgccg
     3061 ctgcaggcgc cggtgcccgg cagtgccgtg atcagctcag ccacgttgaa cgcgccgacg
     3121 ccggtgttgt agtcccagcc cgggttggcg aagaacgcgc cgttggtgcc ggccacgatg
     3181 tcgtagaaac cgggcactgc cgtcgccgga ccagcggtct gcgccagctg gtagatcagc
     3241 ggcgtgatca ggccgtagcc gttgcagctg gcgctctgca cgcgcgccca ggcgcccagc
     3301 gcgagcggcg acgacaggct ggtgccgccg ctgggcagct cgccgtactg ggtggtgaac
     3361 actgcggcgc cggacaccag cgagtcggcg tccatcgcca ggtcgggcgt gccgcgctgg
     3421 ccgatgaccg cgctgggcac gatctcggtg gtctgccatt gcggcgccac ttcgatcagg
     3481 ctcgggccgc cgccgccggc gttccagctg atctctgccg tacgggcatt ggtcgaatcg
     3541 agcgccagcg aggtgcctcc aacgcccacc gcccacggcg acgaagccgg gtaggcctgc
     3601 cccgccggga tgccggagtc cggcacgccg ttggtggaac ccagcggcgt caggccgacc
     3661 accagcccag tgtacgggtc gtagcaagtg gcgccggtgt cgccggtgga ggtgaaaaag
     3721 ctctggccct gcgcggcggc ctgcatcagc accgcgtcgg tggcgtccat gtcgccggcc
     3781 actgcctgca gcacttcgca ggaactcacc gagtagctgg cggccttggc gatgttgtcg
     3841 gcgacgaaac ggttcatcga atccaggtga tcggcattgt tctggaaatc gccgccgtcg
     3901 tagatgacca gcttctgcac gttccccgcc atgccggtcg aggtctgcgt atccatatcc
     3961 cactcgtcct cgccctggtc ggtgctgtgg tcgggagagc cggtcgggat cacgtccacc
     4021 gtcacctgcg gcaggccgaa ggtctgctcg tagctgcgca ggttggcgat gaccgggtcc
     4081 atgttgccga aggccaggat ggcgatggtg gtctggtcgg cggcggcggt gttgccgacg
     4141 ttgtaggccg cccacagctc ctgcggcgac aggccgagcc ccagcaccgt gccatcgatg
     4201 ttgggcaggc ccagcgcggt ctgcctgcgc gccggcagca cgtgctcgac gttattcagg
     4261 ccatagacgc cggcgaccac gccgccgagc gcggcgggca cctgagcatc gcgcgtgttg
     4321 acgaaccagc tgcgcccatc gcgcgcgaac tggcgcagcg tggtgttgaa cgccgcctgc
     4381 acctgcgacg cagttcccga cgcggtcacc agcatgcggt tggaggcgag ctgcacgccg
     4441 gtgaagccgg cctgctgcag gtactcgaca acggcctgcg cctgcagcgc atccggcgcg
     4501 tagcgctggt tgaactgcgc gctggtgagg aaatgcccga acgcgggatt gcccggcgtg
     4561 ttgaccgcgg tggccagtgc cttcaggccc gcagcattgc gcggcttgag cgccagcgcg
     4621 atgtgcatgg cccgtccggc ggccaccggt tgcgcggcaa cgccctgcgc aatcttcagg
     4681 cccttggacg ccgtcgatac ccagcctgcc gcggcatgtg ccgaggcata accgagcatt
     4741 ccggacagca gcagcaacga cgccagccac ccgcgcgcgc gcagattcgt tttcttcatg
     4801 tgcagcctcc tttgcagcag cgcccgcgcg cgagctctgg acggctccta cggcgggcgc
     4861 aagacatggg ttgagtgcag ctttgtgaat cgagtctagt ctgcttaaat gcgccgcaac
     4921 acgggataaa cccctagcca ttctccatga gcgaattccg caccgtcggc gtcatcggca
     4981 agcagctcga cccgcgcgtg ggccccacgc tggcgcggct gtgcgaggac ctgcgcgcgc
     5041 tcggctgcac cctgctggtt gcgccggaat gcgcgccgca tgtcgacgtg tccgccgact
     5101 gcatcgcctc gctggagcag atttcgcagc gagcgaacat tgcggtcgtg gtcggcggcg
     5161 acggtacgct gctgtcggcc gcgcgcgcac tggcgccggc cggcgtgccg atcctgggca
     5221 tcaaccaggg ccggctcggc ttcatggtcg acgtcaatcc caatgagatg aaggagacgc
     5281 tgacgcaggt gttcgccggc gactacgtgc gcgaggaacg cctgctgctg tcggcccggc
     5341 tgcgccgcga cggcgccgtc tccggcccct acctcgcggt caacgacgtg gtgatccgca
     5401 accaggccgc catccgcatg ctcgagttcg agacctggat ggacggctcg ttcatcagcc
     5461 agcaccgcgc cgacggcatg atcgtgtgct cgccgaccgg ctcgactgca tatgcgctgt
     5521 ccggcggcgg gccggtgatg catcccgggc tcgaagcctt cgcgctggtg ccgatctgcc
     5581 cgcacacgct gtccgaccgg cccatcgtgg tcggcagcag gcacctggta cgcatcgtgc
     5641 tcggcggcgc cggcggcacc agcgccatgg tcacctgcga cggccagatc aacgagacgc
     5701 tcaacccggg cgacgcggtg gagatcgagc gcgcgccggc ggcactcagg ctgatccatc
     5761 cgcgcagcta taattacttc gacatcctgc gcagcaagct gcactggggc agagcgcggg
     5821 cttaggaaca aaaaaatgca ggagcgggcc ttgcccgcga ttggttgggt atcgcgggca
     5881 aggcccgc
//
LOCUS       DASZZC010000024         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_837054,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000024 DASZZC010000000
VERSION     DASZZC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837054"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..866
                     /gene="trpA"
                     /locus_tag="VHE37_00605"
     CDS             57..866
                     /gene="trpA"
                     /locus_tag="VHE37_00605"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HVT34053.1"
                     /translation="MNRLQRRFNELRAQGRKALIPYITAGDPHPTQTVGLLHELVKAG
                     ADVLELGVPFSDPMADGPVIQLACERALAHGTGLTQVLDMVAEFRQSDSKTPVVLMGY
                     MNPIETLGVKAFATRAREVGVDGVILVDLALEETPEYLPTLIGQGIDCIFLLAPTSSA
                     ERIAAIAKQASGYLYYVSLKGVTGAATLDVASVAAKLMQIRRQTNLPLCVGFGIRDAA
                     SAKAVGKIADGVVVGSALVSTVEKFQDKPKLLAPALSNILAPMRAALDEIK"
     gene            871..1752
                     /gene="accD"
                     /locus_tag="VHE37_00610"
     CDS             871..1752
                     /gene="accD"
                     /locus_tag="VHE37_00610"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HVT34054.1"
                     /translation="MTSWLEKAISGSKLLTAGVRKRNVPEGLWTKCETCQSVLYRAEL
                     ERTLEVCPKCAAHHPIGARARLNHFLDPEPRHEIGSELAPTDPLKFKDSRKYTDRLKE
                     AQNDTEEKDALIVLRGQLMGLPLVTAAFEFGFMGGSMGSVVGEKFVRGVNAALEYGVP
                     MVCFASSGGARMQESLYSLLQMAKTSAALAKLGERGLPFVSVMTHPTMGGVSASLAML
                     GDINIAEPGALIGFAGPRVIEQTVRQKLPEGFQRAEFLLEHGHIDLIVDRREMRERLY
                     RMLAMLTHFSPSVAGGG"
BASE COUNT          333 a          599 c          620 g          299 t
ORIGIN      
        1 ggccgcggcg acaaggacat cttcaccatc gccaagctcg acggaatcag cctcacatga
       61 accgcctgca gcgacgcttc aacgaactgc gcgcgcaggg ccgcaaggcg ctgatcccgt
      121 acatcacggc gggtgatccg caccctacac aaacggtcgg cctgctgcac gagctggtga
      181 aagccggcgc cgatgtcctg gaactcggcg tgccgttctc cgacccgatg gccgatggtc
      241 ccgtgatcca gctggcctgc gagcgagcgc tggcgcacgg caccggtctc acccaggtgc
      301 tcgacatggt ggcggagttc cgccagtccg acagcaagac gccggtcgtg ctgatgggct
      361 acatgaaccc gatcgagacg ctgggcgtga aggccttcgc cacgcgtgcg cgcgaggtcg
      421 gcgtggatgg cgtgatcctg gtcgacctgg cgctggagga aacgccggag tacctgccga
      481 cgctgatcgg gcagggcatc gactgcatct tcctgctggc accgaccagt tcggccgagc
      541 gcatcgccgc catcgcgaag caggcgagcg gctacctgta ctacgtgtca ctcaagggcg
      601 tcaccggcgc ggccacgctc gatgttgcca gcgtcgcggc caagctgatg cagatccgcc
      661 gccagaccaa cctgccgctg tgcgtcgggt tcggcatccg cgatgcggcc agcgccaagg
      721 ccgtgggcaa gatcgccgat ggcgtggtgg tgggttcggc gctggtgtcg accgtggaga
      781 aattccagga caagcccaag ctgctggcac cggcgctcag caacatcctg gcgccgatgc
      841 gcgccgcact ggatgagata aagtaaggcc atgaccagct ggctcgaaaa agcgatctcc
      901 ggatcgaagc tgctgactgc gggcgtgcgc aagcgcaacg tgcccgaggg gctgtggacc
      961 aagtgcgaaa cctgccagtc ggtgctctac cgcgccgaac tggagcgcac gctggaggtc
     1021 tgccccaagt gcgcggcgca ccatcccatc ggcgcgcgcg cgcggctcaa tcatttcctc
     1081 gatcccgagc cgcgccatga gatcggcagc gagctggcgc cgaccgatcc gctcaagttc
     1141 aaggattcgc gcaagtacac cgaccgtctc aaggaagcgc agaacgacac cgaggagaag
     1201 gacgcgctga tcgtcctgcg cggccagctg atgggcctgc cgctggtcac cgcggcgttc
     1261 gagttcggct tcatgggcgg ttccatgggc agcgtggtcg gcgagaaatt cgtgcgcggt
     1321 gtgaacgcgg ccttggaata cggcgtaccg atggtctgct tcgcctcgtc cggcggagcg
     1381 cgcatgcagg agtcgctgta ctcgctgctg cagatggcca agaccagcgc ggcgctggcc
     1441 aagctgggcg agcgcggcct gccgttcgtc tcggtgatga cgcacccgac catgggcggc
     1501 gtgtccgcca gcctggcgat gctgggcgac atcaacatcg ccgagcccgg cgcgctgatc
     1561 ggttttgccg gtccgcgcgt aatcgagcag accgtgcggc agaagctgcc ggaaggattc
     1621 cagcgcgctg aattcctgct ggagcacggg cacatcgacc tcatcgtcga tcgtcgagaa
     1681 atgcgcgaac ggctctaccg catgctcgcc atgctcacgc atttttcgcc gagcgttgcc
     1741 ggcggcggat gatgcgatcc gatccttgac ttgaggggac gcgtccctac aatacgcacc
     1801 ctgcaacgcg ggaatagctc agttggtaga gcgcaacctt gccaaggttg a
//
LOCUS       DASZZC010000025         4937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_708817,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000025 DASZZC010000000
VERSION     DASZZC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708817"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHE37_00615"
     CDS             complement(<1..237)
                     /locus_tag="VHE37_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34055.1"
                     /translation="MPRDYAQSTGSPRHTAPRKSKGKGAGLPSWVWLFAGLSVGLAVA
                     AFVYMSRPVAPMPASAGAETSEAMPAKPAVKAGKP"
     gene            complement(237..2000)
                     /gene="argS"
                     /locus_tag="VHE37_00620"
     CDS             complement(237..2000)
                     /gene="argS"
                     /locus_tag="VHE37_00620"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HVT34056.1"
                     /translation="MKSHLQDLLRGALQKTLAATGGTAAPAIAVDATKDPKLGDFQTN
                     LALQLAKPLGKPPRAVAEMLVANLPASPRVKSVNIAGPGFINFFVADAALQSVVSDIL
                     AAGAAYGRDDSGSKGKVMVEFVSANPTGPMHVGHGRNAAYGDALSNLLAATGWQVWRE
                     YYINDAGRQIDVLTASVWLRYLQSAKYEVPMPVRGYVADYINVCAKKLRAAFGNRFER
                     SNEDYLRDLPEEPATAKDDETLKKGLEDYLDAVIARCKMLLGNDYEVLRKFAYEDQLG
                     IIKQTLADFGVRFDQWYSERELVESGLAQKRRGELIASGHTYEKDHALWFRTTEHGDD
                     KDRVLVKADGAATYFGNDLAYHVDKLDRGWSQLIDVWGADHHGLLVRMRAALESLTGR
                     KDALRVQLIQFVTLSSGKMGKRSGNFVTLADLIADAGKDATRFFYLMRSHDQHLEFDI
                     DLARSQSTDNPVYYVQYAHARICSVYAQLQEKGGVYDRAAGLAALQRLDTEHEKRLLT
                     ALARWPEALRNAATNHAPHLLVHYLKDEVADALHAYYHAHRFIIDDAPLQAARLALIE
                     AARVVLASGLALLGVSAPEKM"
     gene            complement(2048..3466)
                     /locus_tag="VHE37_00625"
     CDS             complement(2048..3466)
                     /locus_tag="VHE37_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT34057.1"
                     /translation="MRCAILLAALWMADAPAATLAIEHIHLVDATGAPARSGITVLVR
                     DARIAQIAPDGHVQIPADAQRVDGTGRWLIPGLWDMHVHVLRPGRPDAYFPLLVAQGI
                     VGVRDMGGDLPLAEVRRLKDAIARGARTGPQLVAAGRILDGPPMQLPQISTLVTDAAS
                     AQAAVQRAHDDGADFIKVYNRLSRPAYFSIATAARKLGLPVAGHVPFSVSAREASNAG
                     QASIEHLFNILFACSSREDELMARKADTLGPGIAGERREQRRAYLRDVLDSYDAPRAQ
                     ALFALFARNHTWQVPTLVQRRAFGEPDTWQADAQQLQYVPQTQRWRWDVQQDGRIANR
                     EAEDRELERRFYEKDREQIPPMLRVGVGFLAGTDAGDPYSVPGFSLHQELALLVDAGL
                     TPLQALQAATREPARFLHREREQGTVETGKRADLVLLDADPLADIHNTSHIAGVVLDG
                     RWLDRAALDVLLAQARAAAAAN"
     gene            complement(3477..4391)
                     /gene="aroE"
                     /locus_tag="VHE37_00630"
     CDS             complement(3477..4391)
                     /gene="aroE"
                     /locus_tag="VHE37_00630"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HVT34058.1"
                     /translation="MTDQYAVIGCPISHRKSPVIHARFAAQVGAQLSYIAIEIRPDAL
                     AARLAELHAQNFLGLNVTLPHKTAVAAICNNVSERAQVAGAVNTLVRTAGGWDGDNTD
                     GEGLIRDLTVNLGIGVAGKRVLVLGAGGAARGILKPLLDQQPAELTLSNRNPWKPEEL
                     AIAFRPHGVIRPATHLALKGDHYDLVINATSAGHQGKLPKLPPQLLAPGAACYDMSYG
                     AAAEPFCAWARANGAAKVSDGLGMLVEQAAASFERWRGVRPQAAPVIAELRAAAIAAE
                     PAQDDGKPREAATHRVHGEDCGRPAAAG"
     gene            complement(4388..>4937)
                     /locus_tag="VHE37_00635"
     CDS             complement(4388..>4937)
                     /locus_tag="VHE37_00635"
                     /inference="COORDINATES: protein motif:HMM:NF022041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT34059.1"
                     /translation="DGDTLIADSHSIIDHLARTRGKDLDAHLSARERAQALAWERLLA
                     EHLYWSMVYFRWVDDSGWQRIRKYFFSPIPAPVRPLVEFAARRGTRAQTRGHGLGRHA
                     PQEIVRRAGEDIDALAAQLGEQPFLVGAQPTRLDATAYALLANIWQIDFDTPLKPLVA
                     RHANLRAYCERMRARCFPEPTS"
BASE COUNT          716 a         1666 c         1745 g          810 t
ORIGIN      
        1 tggcttgccg gccttcaccg ccggtttggc cggcattgct tcgctggttt ccgcgccggc
       61 ggaggccggc atcggcgcca ccgggcgcga catgtagacg aacgcggcca ccgccaggcc
      121 caccgacagc ccggcgaaca gccacaccca cgacggcagg ccggcgccct tgcccttgct
      181 cttgcgcggc gccgtgtggc gcgggctgcc ggtggactgg gcgtaatcgc gtggcatcac
      241 attttctccg gcgccgaaac acccagcagg gccaggccgc tggccagcac cacgcgcgcc
      301 gcttcgatca gcgccagccg cgcagcctgc agcggcgcgt cgtcgatgat gaagcggtgt
      361 gcgtggtagt aggcgtgcag cgcatcggcc acttcgtcct tcaggtaatg caccagcagg
      421 tgcggcgcgt ggttggttgc agcgttgcgc agcgcctccg gccagcgcgc cagcgcggtg
      481 agcaggcgct tctcatgctc ggtgtcgagc cgctgcagcg ccgccagccc cgccgcgcga
      541 tcgtacacgc cgcccttctc ctgcagctgt gcgtacacgc tgcagatgcg cgcgtgcgca
      601 tattgcacgt agtacaccgg gttgtcggtc gactgcgaac gcgccaggtc gatgtcgaac
      661 tcaaggtgct ggtcgtgcga gcgcatcagg tagaagaagc gggtggcatc ttttccggcg
      721 tcggcgatca ggtcggccag ggtgacgaag ttgccgctgc gcttgcccat cttgccgctc
      781 gacagcgtga cgaactggat cagctgcacc cgcagcgcat ccttgcgccc ggtgagcgac
      841 tccagcgccg cgcgcatgcg caccagcagg ccgtgatggt ccgcgcccca tacatcgatc
      901 agctggctcc agccgcggtc gagcttgtcg acgtggtagg cgaggtcatt gccgaaatag
      961 gtggccgcgc catcggcctt gaccagcacg cggtccttgt catcgccgtg ctcggtggtg
     1021 cggaaccaca gcgcgtggtc cttctcgtag gtgtggccgg aggcgatcag ctcgccgcgg
     1081 cgcttctgcg ccaggcctga ctccaccagc tcgcgctcgg aataccactg gtcgaagcgc
     1141 acgccgaagt cggccagggt ctgtttgatg atgcctagtt gatcttcgta agcaaatttt
     1201 ctgaggactt cgtagtcgtt gcccaggagc atcttgcatc gagcgataac ggcatcgaga
     1261 tagtcttcga gccccttctt gagagtctca tcgtctttgg cagttgccgg ctcttcaggc
     1321 aagtcacgaa gatagtcttc gttcgagcgc tcaaatcgat tgccgaatgc ggcgcgcagt
     1381 tttttggcgc agacattgat gtagtcagcc acgtagccgc gaaccggcat ggggacttca
     1441 tattttgctg actggagata ccgcagccac accgaagctg taagcacgtc gatctgccgg
     1501 ccggcatcat tgatgtagta ctcgcgccac acctgccagc cggtggcagc gagcaggttg
     1561 gacagcgcat cgccgtaagc cgcgttgcgg ccatggccta cgtgcatcgg cccggtggga
     1621 ttggcggaga cgaactccac catcaccttg cccttgctgc cgctgtcgtc gcggccgtac
     1681 gcagcgccgg cagcgaggat gtcggaaacc accgactgca gcgcggcgtc ggcgacgaag
     1741 aaattgatga agcccggccc ggcgatgttc acgcttttca cgcgcggcga ggccgggaga
     1801 ttcgccacca gcatttccgc caccgcgcgc ggcggcttgc ccagcggctt ggccagctgc
     1861 agcgccaggt tggtctggaa atcgcccagc ttcgggtcct tggtggcgtc caccgcgatg
     1921 gcgggcgcag ccgtgccgcc ggttgcggcg agcgtcttct gcagcgcgcc gcgcaacagg
     1981 tcttgcaggt gtgacttcat cgagcgcgga ttttacctgc aatgactccg cggggctgct
     2041 gcagcggtca gttcgccgcc gccgccgcgc gcgcctgcgc aagaagaacg tccagcgcgg
     2101 cgcgatcgag ccagcgtccg tccagcacga cgcccgcgat gtgggacgtg ttgtggatgt
     2161 cggccagcgg gtcggcgtcg agcagcacga ggtcggcgcg cttgccggtc tccaccgtgc
     2221 cctgctcgcg ttcgcggtgc aggaagcgcg cgggctcgcg cgtggcggcc tgcagcgcct
     2281 gcagcggcgt cagcccggca tcgaccagca gcgccagttc ttgatgcagg gaaaatcccg
     2341 gcacgctgta cggatcgccg gcatcggtgc cggcgaggaa gccgacgccg acgcgcagca
     2401 tcggcggaat ctgttctcga tccttttcat agaagcgccg ctcgagttcg cggtcttccg
     2461 cctcgcggtt ggcgatgcgg ccgtcctgct gcacgtccca gcgccagcgc tgggtctgcg
     2521 gcacgtattg cagctgctgc gcatcggcct gccaggtgtc cggttcacca aacgcgcgcc
     2581 gctgtaccag cgtcggcacc tgccaggtgt ggttgcgcgc gaacagcgca aacagggcct
     2641 gcgcgcgcgg cgcgtcgtag ctgtcgagca catcgcgcag gtaggcgcgg cgctgctcgc
     2701 ggcgctcgcc ggcgatgccg gggccgagcg tgtcggcctt gcgcgccatc agctcgtctt
     2761 cgcgcgagga gcaggcgaac aggatgttga acaggtgctc gatgctggcc tgccccgcgt
     2821 tcgaggcctc gcgcgccgaa accgaaaacg gcacatggcc cgcaaccggc aggccgagtt
     2881 ttctcgccgc ggtggcgatg gagaaatacg ccggccgcga cagccggttg tagaccttga
     2941 tgaagtcggc gccgtcgtcg tgcgcgcgct gcaccgcggc ctgcgcggac gcggcgtctg
     3001 tgaccagcgt ggagatctgc ggcagctgca ttggcgggcc gtcgaggatg cggccggcgg
     3061 ccaccagctg cggcccggtg cgagcgccgc gcgcaatcgc gtccttgagc cgccgcactt
     3121 cggccagcgg caggtcgccg cccatgtcgc gcacgccgac gatgccctgc gccaccagca
     3181 gcggaaagta cgcgtcgggc cggccggggc gcagcacgtg cacgtgcatg tcccacaggc
     3241 ccgggatcag ccagcggccg gtgccatcga cacgctgcgc atcggcagga atctgcacat
     3301 gaccatcggg cgcgatctgc gcgatgcgcg cgtcgcgcac cagcacggtg atgccgctgc
     3361 gcgcgggcgc gccggtggcg tcgaccaggt ggatgtgctc gatggccagc gtcgcggccg
     3421 gcgcatcggc catccacagc gctgcgagca ggatcgcgca gcgcatgagg ccgcgctcag
     3481 cccgcggcgg cgggcctgcc gcagtcctcg ccgtgtacgc gatgcgtcgc agcctcgcgc
     3541 ggcttgccgt catcctgcgc cggctcggcg gcaatggcgg cggcgcgcag ttcggcgatc
     3601 accggcgccg cctgcgggcg cacgccgcgc cagcgctcga acgaggcggc cgcctgttcc
     3661 accagcatgc ccaggccgtc gctgactttc gccgcgccgt tggcgcgcgc ccaggcgcag
     3721 aacggttcgg ctgcggcgcc gtagctcatg tcgtaacagg cggcgcccgg cgcgagcagt
     3781 tgcggcggca gcttgggcag cttgccctgg tggccggcgg aagtggcgtt gatcaccagg
     3841 tcgtaatgat cgcccttgag cgcgaggtgg gtggccgggc ggatcacgcc gtgcggcctg
     3901 aaggcgatgg cgagctcttc gggtttccac ggattgcggt tggacagcgt gagttccgcc
     3961 ggctgctggt cgagcagcgg cttgaggatg ccgcgcgccg cgccgccggc gcccagcacc
     4021 agcacgcgct tgccggccac gccgatgccc aggttcacgg tgaggtcgcg gatcagccct
     4081 tcgccgtcgg tgttgtcgcc gtcccagccc ccggcggtgc gcaccagcgt gttgaccgcg
     4141 ccggccacct gcgcccgttc gctgacgttg ttgcagatgg ccgccaccgc ggtcttgtgc
     4201 ggcagggtga cattgaggcc gaggaaattc tgcgcgtgca gttccgccag gcgcgcagcc
     4261 agcgcgtcgg gcctgatctc gatggcgatg tacgacagct gcgcgcccac ctgcgcggcg
     4321 aagcgcgcgt ggatcaccgg cgacttgcgg tgcgagatgg ggcagccgat gaccgcgtac
     4381 tggtccgtca tgaggtgggt tccgggaagc agcgcgcgcg catgcgctcg caatatgcgc
     4441 gcaggttggc gtggcgcgcc accagcggct tgagcggcgt gtcgaagtcg atctgccaga
     4501 tgttggccag cagcgcgtag gcggtggcgt cgagccgcgt gggctgcgcg cccaccagga
     4561 acggctgctc gccgagttgc gcggccagcg catcgatgtc ttcgccggcg cgacgcacga
     4621 tctcctgcgg cgcatggcgg cccaggccat gcccgcgcgt ctgcgcgcgc gtgccgcggc
     4681 gcgcggcgaa ttccaccagc ggccgcaccg gcgccgggat cggcgagaag aaatatttgc
     4741 ggatgcgctg ccagccggag tcatcgaccc agcggaaata caccatggac cagtacaggt
     4801 gctcggccag gaggcgttcc caggccagcg cctgcgcgcg ttcgcgcgcg gacaggtgcg
     4861 cgtcgagatc cttgccgcgc gtgcgcgcca ggtgatcgat gatgctgtgc gaatcggcga
     4921 tgagcgtgtc gccgtcg
//
LOCUS       DASZZC010000026         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_964534,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000026 DASZZC010000000
VERSION     DASZZC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964534"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHE37_00640"
     CDS             <1..273
                     /locus_tag="VHE37_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34060.1"
                     /translation="ERIELAKLQNPQVMKTMKVPNMTERLKEITCPALTLWGMNEAMM
                     PDSGILRIGKGLPHGRMVLVPQCGHWVMIEHRALFNRMVLDFLNNG"
     gene            277..1074
                     /locus_tag="VHE37_00645"
     CDS             277..1074
                     /locus_tag="VHE37_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVT34061.1"
                     /translation="MALPATQIAQLGDELYAALRARSVLDPLTTRVPAITIEDAYQVS
                     LRLLERRKAEGERVIGKKIGVTSKPVQDMLGVHQPDFGFLTDRMHIADGAAISLKSTP
                     MIQPRAEGEIAFVLKKDLRGPGVTEEQVLDATEYVSPCFEIVDSRIRDWKIKIQDTVA
                     DNASCGVFVIGKDRIAPRALDLAAVKMEITKNGAHAASGIGSAVQGHPATAVAWLANT
                     LGAFGIPFLAGEIILSGSLAPLLPAAPGDKFAMTLAGIGGASVSFIE"
     gene            1079..1963
                     /locus_tag="VHE37_00650"
     CDS             1079..1963
                     /locus_tag="VHE37_00650"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HVT34062.1"
                     /translation="MKKIRCAMIGSGNIGTDLMYKLQRSTVLEPVWMVGIEATSEGLA
                     RAKSMGLKTTDKGVDGLLPHVKADNIQIAFDATSAYVHRENSDKLTKLGVKVIDLTPA
                     AIGPFCVPPVNLKEHLGKNEMNVNMVSCGGQATIPMVYAVSRVQAVKYGEIVATVASK
                     SVGPGTRKNIDEFTRTTASGVEKVGGAKQGKAIIVINPAEPPMIMRDTIHCLTEEAPK
                     QDEITRSVHEMIKEVQKYVPGYVLKNGPVFDGNRVTVFMEVAGLGDYLPKYAGNLDIM
                     TASATRTAEMFAEEMLKA"
BASE COUNT          412 a          614 c          645 g          300 t
ORIGIN      
        1 gagcgcatag agctggccaa gctgcagaac ccgcaggtga tgaagaccat gaaggtgccc
       61 aacatgacgg agcgcctgaa ggagatcacc tgccccgcgc tgacgctgtg gggcatgaac
      121 gaggcgatga tgccggacag cggcatcctg cgcatcggca agggcctgcc gcacgggcgc
      181 atggtgctgg tgccgcaatg cgggcactgg gtgatgatcg agcatcgcgc gctgttcaac
      241 cgcatggtcc tcgactttct caacaacggc tgaaccatgg cgctgcccgc aacacagatc
      301 gcgcaactgg gcgatgagct ttacgccgcg ctgcgcgcgc gcagcgtgct cgacccgctg
      361 accacgcgcg tgccggcgat caccatcgaa gatgcctacc aggtttctct gcgcctgctg
      421 gaacggcgca aggccgaggg cgagcgcgtg attggcaaga agatcggcgt caccagcaaa
      481 cccgtccagg acatgctcgg cgtgcaccag ccggatttcg gattcctcac cgaccgcatg
      541 cacatcgccg acggcgccgc tatcagcttg aaaagcacgc cgatgatcca gccgcgggcc
      601 gagggcgaaa tcgccttcgt gctgaagaag gacttgcgcg gccccggcgt gaccgaggaa
      661 caggtgctgg acgcaaccga gtacgtctcg ccctgcttcg agatcgtcga ctcgcgcatc
      721 cgcgactgga agatcaagat ccaggacacc gtggccgaca acgcttcgtg cggcgtgttc
      781 gtcatcggca aggaccgcat cgcgccgcgc gcgctggatc ttgccgcggt gaagatggag
      841 atcaccaaga acggcgcgca tgccgcgagc ggaattggct cagcggtgca ggggcatccg
      901 gcgacggcag tggcgtggct ggccaatacg ctgggtgcgt tcggcattcc atttctcgcc
      961 ggcgaaatca tcctgtccgg ctcgttggcg ccgctgcttc ccgcagcgcc cggcgacaaa
     1021 ttcgcaatga cgctcgccgg catcggcggc gcgtccgtct ctttcatcga gtgaggcaat
     1081 gaagaaaatc agatgcgcca tgatcggctc cggcaacatc ggcaccgacc tgatgtacaa
     1141 gctccagcgc agcacggtgc tggagccggt gtggatggtc ggcatcgagg cgacgtccga
     1201 aggcctcgcc cgcgccaagt ccatgggcct gaagaccacg gataaaggcg tcgatggcct
     1261 gctgccgcac gtcaaggccg acaatatcca gatcgcgttc gacgccacca gcgcctacgt
     1321 gcacagggaa aactcggaca agctgacgaa gctcggggtg aaggtgatcg acctcacgcc
     1381 cgccgccatc ggccccttct gcgtgccgcc ggtgaacctg aaggaacatc tcggcaagaa
     1441 cgagatgaac gtcaacatgg tcagctgcgg cggccaggcg accatcccca tggtctacgc
     1501 ggtgtcgcgc gtgcaggcgg tgaagtacgg cgagatcgtg gcgacggtgg cgagcaaatc
     1561 cgtcggcccc ggcacgcgca agaacatcga cgagttcacc cgcaccacgg cgagcggcgt
     1621 ggaaaaagtc ggcggcgcca agcagggcaa ggcgatcatc gtgatcaacc cggccgagcc
     1681 gccgatgatc atgcgcgaca ccatccactg cctgaccgag gaagcgccga agcaggatga
     1741 gatcacgcgc tcggtgcacg agatgatcaa ggaagtgcag aaatacgtgc ccggctacgt
     1801 gctgaagaac ggaccggtgt tcgatggcaa ccgcgtcacc gtgttcatgg aagtggcggg
     1861 cctgggcgat tacctgccca agtacgcggg aaacctggac atcatgacgg cgagcgcgac
     1921 gcgcacggcc gagatgttcg ctgaagaaat gctgaaggct tgaagtttac t
//
LOCUS       DASZZC010000027         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_130735,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000027 DASZZC010000000
VERSION     DASZZC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130735"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VHE37_00655"
     CDS             complement(<1..626)
                     /locus_tag="VHE37_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HVT34063.1"
                     /translation="MRLALFALLAAGAPAAFAADLPAANSGDTAWMLTSTALVLFMTL
                     PGLALFYGGLVRTKNVLSVLVQCFAITVVVSLLWLVYGYSLAFAPGSGFIGGLDKAMF
                     AGVGRSSVSGSIPETAYALFQLTFAVITPALVIGAFAERMRFSAMLLFSTLWFTAVYL
                     PVCHWVWAGDGWLFKRGILDFAGGTVVHVTGGVGALVAALALGPRQGFP"
     gene            complement(689..>1879)
                     /gene="ettA"
                     /locus_tag="VHE37_00660"
     CDS             complement(689..>1879)
                     /gene="ettA"
                     /locus_tag="VHE37_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HVT34064.1"
                     /translation="PVTNLSGGEKRRVALARLLLQKPDILLLDEPTNHLDAESVAWLE
                     RTLQDYPGTVVAVTHDRYFLDNVAGWILELDRGTGIPWQGNYSSWLEQKTNRLKEEQN
                     KEDARQKALAEELEWVRKNPKGRQAKSKARIKAYEELASQEYQTRSETKELYIPPGPR
                     LGDVVIEVNNLGKKFGDRELYSGVNFNVPKGAIVGIIGANGMGKTTLFRILTGQDKDY
                     QGEVKIGETVKFAYVDQSRDSLNGENNVFKEISGGNDILKIGNYEMPSRAYIGRFNFK
                     GEAQNKRIKELSGGERNRVHLAKLLLTGGNVVLLDEPTNDLDVETLRALEEAILDFPG
                     CALVISHDRWFLDRVATHILAFEDSGEVIWHEGNYRDYEDDFRRRTGSEPGVNRRARH
                     KKLA"
BASE COUNT          313 a          596 c          633 g          337 t
ORIGIN      
        1 ggaaaaccct ggcgcggccc gagcgccagc gccgccacca gcgcgccgac gccgccggtc
       61 acgtgcacca cggtgccgcc ggcgaaatcg aggatgccgc gcttgaacag ccagccatcg
      121 ccggcccaca cccagtggca caccggcagg tacaccgcgg tgaaccacag cgtggagaac
      181 agcagcatgg cggagaagcg catgcgctcg gcaaaggcgc cgatgaccag cgccggcgtg
      241 atcaccgcga aggtcagctg gaacaatgca tacgcggttt caggaatgct gccgctcacc
      301 gagctgcggc cgacgccggc gaacatggcc ttgtccagac cgccgatgaa gccgctgccc
      361 ggcgcaaagg ccaggctgta gccgtacacc agccacaaaa ggctgaccac gacggtgatg
      421 gcgaagcact gcaccagtac cgacagcacg ttcttggtcc gcaccagccc gccgtagaac
      481 agcgcgagcc cgggcagcgt cataaacagc accagcgcgg tgctggtcag catccacgcg
      541 gtatcaccgc tgttggcggc aggcagatcg gcggcaaacg cggctggcgc gccggcggcc
      601 agcagcgcga acaaggcaag gcgcatcggt tcccccagag aggcgcgcac cataagagat
      661 gcgcgtgcac cgaacaagtg ccccgcgatc aggccagctt cttgtgcctg gcgcggcggt
      721 tgacgcccgg ctcgctgccg gtgcggcggc ggaagtcgtc ctcgtagtcg cggtagttgc
      781 cctcgtgcca gatcacctcg ccggaatcct cgaaggcgag gatgtgcgtg gcgacgcggt
      841 cgaggaacca gcggtcgtgc gagatgacca gcgcgcagcc ggggaaatcc aggatggctt
      901 cttccagtgc gcgaagggtt tccacgtcga ggtcgttggt cggctcgtcg agcaaaacca
      961 cattgccgcc ggtgagcagc aatttcgcca ggtgcacgcg gttgcgctcg ccacctgaga
     1021 gttccttgat gcgcttgttc tgcgcctcgc ccttgaagtt gaagcggccg atgtaggcgc
     1081 ggctgggcat ctcgtagttg cctatcttga ggatgtcatt gccgccggag atttccttga
     1141 acacgttgtt ctcgccattg agcgagtcgc ggctctggtc gacgtacgca aatttcaccg
     1201 tctcgccgat cttcacctcg ccctggtaat ccttgtcctg gccagtgagg atgcggaaca
     1261 gcgtggtctt gcccatgccg ttggcgccga tgatgccgac gatggcgccc ttgggcacgt
     1321 tgaagttcac gccggagtag agctcgcggt cgccgaattt cttgccgagg ttgttgacct
     1381 cgatcaccac gtcgcccagg cgcgggccgg gcgggatgta cagttccttg gtctcgctgc
     1441 gcgtctggta ttcctgcgag gcgagttcct cgtaggcctt gatgcgcgcc ttgctcttgg
     1501 cctggcggcc tttcggattc ttgcgcaccc attcgagttc ttcggccagc gccttctgcc
     1561 gcgcgtcttc cttgttctgc tcttccttca ggcggttggt cttctgctcc agccagctgg
     1621 agtagttgcc ctgccagggt atgccggtgc cgcggtccag ctcgaggatc cagccggcca
     1681 cgttgtccag gaagtagcgg tcgtgggtaa cggccacgac cgtgcccggg tagtcctgca
     1741 gagtccgctc gagccaggcg acggactcgg cgtcgaggtg gttggtgggc tcgtcgagca
     1801 ggaggatgtc gggcttctgg aggaggaggc gggcgagggc gacccggcgt ttttcgccgc
     1861 cggagaggtt ggtgacggg
//
LOCUS       DASZZC010000028         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_2505,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000028 DASZZC010000000
VERSION     DASZZC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2505"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VHE37_00665"
     CDS             <1..927
                     /locus_tag="VHE37_00665"
                     /inference="COORDINATES: protein motif:HMM:NF022470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HVT34065.1"
                     /translation="AMAYWHFFPAADGWGRVPVWGFANVARSRCAELAEGERVYGYLP
                     MSTHLLVQPARVTPANFVDASAHRAKLPAAYQQYRRCSGDPGYRREFEDQQALLFPLY
                     ITSFLIEDFLADNQLFGARRVVLASASSKTALGLAFLLRRNRPDGCVVTGLTSARNRA
                     FCQSTGYYDEVLGYEDVAQLPREAAVFVDMAGDGKLLHAVHHHYADALRHSCIVGATH
                     WEQRSTQHALPGPKPQFFFAPDRIRKRAQDWGPGGIETRFADAWRALLPSVAGWLQVT
                     HARGATEIEASYRDVLEGRAQPQQGHILSFAR"
     gene            complement(1061..2266)
                     /locus_tag="VHE37_00670"
     CDS             complement(1061..2266)
                     /locus_tag="VHE37_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT34066.1"
                     /translation="MIHLEIPKKFTVLVQQAQQVAQQMFRPISRKYDRAEHAYPKELD
                     MLAALLDGTKDGGGAAGAGAAPVRGAAVEEGNKNGGNMASVLGTMELCWGDVGLLLSL
                     PRQGLGNAAIGAVANEEQQKRFAGRWAAMAITEPGAGSDSAAIRTTAVKDGDFYVLNG
                     EKIFVTAGERAELVVVWATLDKNAGRAAIKSFVVEKGTPGFELVRLEHKLGIRSSDTA
                     AFVLRDCRVPKENLLGSPEVDVSKGFAGVMQTFDNTRPMVAAMAIGLTRACLDETRRI
                     LADAGVDISYDAPACTQSAAAAQYLSMEADYEAARLLALQAAWMADNRKPNSLQASMA
                     KAKAGRVCVDVALKCVELCGSAGYAEGSLLEKWARDAKILDIFEGTQQIQLLIVARRI
                     LGKTSSELK"
     gene            complement(2263..3579)
                     /locus_tag="VHE37_00675"
     CDS             complement(2263..3579)
                     /locus_tag="VHE37_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT34067.1"
                     /translation="MNAYQRVMGAGLKALNRWAGSPLIDRLGLRKASERAVYHASRTG
                     FRTAGTVGRSFVAAQKLLKPARPARAKSGDLFDLTPTDEQQALREAVLNFALNELRPV
                     AAQADHDCAAPAALLQQSAELGLTLMNVPEELGGAGNERNAVTNALVTEALAQGDMGL
                     AYAVLAPSAVSNALVLWGDEQQQAKYLPAFVGDRQPAAAFAMLEPCALFNPFELATTA
                     RRSGEHYVLSGVKSLVARAAQAELFIVAAAIEGAGPALFIVESKTPGIGIEAEPAMGL
                     RAAATGRVILGDVKVPVSALLAGGATEIYEECVQLSRAGWCALAVGTAQATLDYVTQY
                     TRERMAFGEPIAHRQSVAFAVANMAIELEGMRLLTWRAASRADEGKLFSREAALARRA
                     CADRGMVIGSDGVQLLGGHGFVKDHPVERWYRDLRAAGVMEGGVLL"
     gene            complement(3576..4052)
                     /locus_tag="VHE37_00680"
     CDS             complement(3576..4052)
                     /locus_tag="VHE37_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34068.1"
                     /translation="MKVDLAIERSLDLEAPFERVTGLLEDYETTIRRFPKLRKLKKLG
                     DMTYLWEMAPIGSRIANISHEVVYAARYQVDAPRGRLSWQGMPSHGNAVIHGEFELKP
                     ARKGTRLRFAVNGQLRDVPVPLIYRLVAPPFIQGKFTRLVDVFLEKTQQALEGEDA"
     gene            complement(4154..>4869)
                     /locus_tag="VHE37_00685"
     CDS             complement(4154..>4869)
                     /locus_tag="VHE37_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HVT34069.1"
                     /translation="GLSRTAGFCPLTFAIVEYDADQEAPARDAQGYMRKVEKGGVGLL
                     ISEVTERNPFDGYTDAKASEAKLFRDVFEKGDCWFNTGDLVRDQGFRHIQFVDRTGDT
                     FRWKGENVATTEVEGALNGFPGVHEAVVYGVQLPGADGRAGMAALTYKGADFPGAELA
                     HHLLVALPPYAVPVFLRLREEQEVTGTFKFRKVELKQQGIDPQACGEPLYVLLDRERG
                     YEPLTQALHRQIMQGQIRF"
BASE COUNT          739 a         1729 c         1559 g          842 t
ORIGIN      
        1 gccatggcct actggcattt ctttccggcc gccgacggct ggggccgcgt gccggtgtgg
       61 ggcttcgcca acgtggcgcg ctcgcgctgc gccgaactgg ccgagggcga gcgcgtctac
      121 ggctacctgc ccatgtcgac gcacctgctg gtgcagcccg cgcgcgtcac gccggcgaat
      181 ttcgtcgacg ccagcgcgca tcgcgccaag ctgcccgccg cctatcagca atatcgccgc
      241 tgctcgggcg acccgggcta tcgccgggaa ttcgaggacc agcaggcgct gctgttcccg
      301 ctctacatca cctcgttcct gatcgaggat ttcctggccg acaaccagtt gttcggagcc
      361 aggcgcgtcg tgctggccag cgcgtcatcg aagaccgcgc tcggcctcgc cttcctgctc
      421 aggcgcaacc ggccggatgg ctgcgtggtc accgggctca cctccgcgcg caaccgggcg
      481 ttctgccaaa gcaccggcta ctacgacgag gtgctgggct acgaagacgt ggcgcagctt
      541 ccgcgcgagg ccgcggtgtt cgtcgacatg gccggcgacg gcaagctgct gcatgccgtg
      601 caccaccact atgccgacgc gctcaggcac agctgcatcg tcggcgccac gcactgggaa
      661 cagcgctcca cgcagcacgc cctgccggga cccaagccgc agttcttctt cgcgcccgac
      721 cgcatccgca agcgcgcgca ggactggggc ccgggcggca tcgaaacgcg tttcgccgat
      781 gcctggcgcg cgctgctgcc gtcggtggcc ggctggctgc aggtgacgca cgcacgcggc
      841 gctacggaaa tcgaagcaag ctaccgcgac gtgctggaag gccgtgctca gccgcagcag
      901 gggcacatcc tctcgttcgc gcgctgaatt tcgcgtcgaa caggcgcgaa aaacagttca
      961 ccgcactgcc attcttggtg ttattaaata tcgtctgttt gttatttcat ataatttttt
     1021 ataacacgta aaacagcagt gcggtcagac ttctgcaagc ctatttcagc tcgctggagg
     1081 tcttgccgag gatgcgccgc gccacgatca gcagctggat ctgctgcgtg ccttcgaaga
     1141 tgtcgaggat cttggcatcg cgtgcccact tttccagcag cgaaccttcc gcgtagccgg
     1201 cgctgccaca cagctcgacg cacttgagcg cgacgtccac gcacacgcgc cccgccttgg
     1261 ccttggccat cgacgcctgc agcgaattcg gcttgcggtt gtcggccatc cacgcggcct
     1321 gcagcgcgag caggcgcgcc gcttcgtagt cagcttccat cgacaggtac tgcgccgccg
     1381 ccgcactctg cgtgcaggcc ggcgcgtcgt agctgatgtc cacgccagcg tccgcgagga
     1441 tgcgccgcgt ttcgtccagg caggcgcgcg tcaacccgat ggccatcgcc gccaccatcg
     1501 gccgcgtgtt gtcgaaggtc tgcatcacgc cggcgaaacc cttgctcacg tccacctcgg
     1561 gactgccgag caggttttcc ttcggcacgc ggcaatcgcg caggacgaag gccgcggtgt
     1621 cggaagaacg gatgccgagc ttgtgctcca gccgcaccag ctcgaagccc ggcgtgccct
     1681 tctccaccac gaagctcttg atcgcggcgc ggccggcgtt cttgtccagc gtggcccata
     1741 ccaccaccag ttccgcgcgc tcgccggcgg tgacgaaaat cttctcgccg ttaagcacgt
     1801 agaaatcgcc atccttcacg gcggtggtgc ggatggccgc ggagtccgat ccggcgcccg
     1861 gctcggtgat ggccatcgcc gcccagcgcc cggcgaagcg cttctgctgt tcctcgttag
     1921 ccacggcgcc gatggcggcg ttgcccagcc cctgccgcgg cagcgacagc agcaggccga
     1981 cgtcgcccca gcacagttcc atggtaccga gtaccgaggc catgttgccg ccgttcttgt
     2041 tgccctcctc gactgccgcg ccgcgcaccg gcgccgcacc ggcacccgcc gcgccgccgc
     2101 catccttggt gccgtcgagc agcgccgcca gcatgtcgag ctcttttgga taggcatgct
     2161 cggcccggtc gtacttgcgc gagatgggcc ggaacatctg ctgcgccacc tgctgcgcct
     2221 gctgcaccag cacggtgaac ttcttcggga tttcgagatg gatcacagca gcactccgcc
     2281 ttccatgacg cctgcggcgc gcaggtcgcg gtaccagcgc tcgaccgggt gatccttgac
     2341 gaagccgtgc ccgccgagca gctgcacgcc gtcgctgccg atgaccatgc cgcgatcggc
     2401 gcaggcgcgg cgcgccagag cggcttcgcg cgagaacagc ttgccctcgt cggcgcgcga
     2461 tgcggcgcgc caggtgagca ggcgcatgcc ttccagctcg atcgccatgt tggccaccgc
     2521 gaaagccacc gattgccggt gcgcgatcgg ctcgccgaac gccatgcgct cgcgcgtgta
     2581 ttgcgtcacg tagtcgagcg tggcctgcgc ggtgccgacg gcgagcgcgc accagcccgc
     2641 acgcgacagt tgcacgcatt cctcgtagat ttccgtcgcg ccgccggcga gcagcgcgga
     2701 taccggcacc ttgacgtcgc ccaggatcac gcgcccggtc gctgccgcgc gcaggcccat
     2761 cgccggctcg gcttcgatgc cgatgcccgg cgtcttcgat tcgacgatga acagcgcggg
     2821 ccccgcgcct tcgatggctg ccgccacgat gaacagctcg gcctgcgcgg cgcgcgccac
     2881 cagcgatttc acgccgctca gtacgtagtg ctcgccgctg cgccgcgccg tggtggcgag
     2941 ttcaaacgga ttgaacagcg cgcacggctc gagcatggcg aacgccgctg ccggctgccg
     3001 gtcgccgacg aaggccggca ggtatttcgc ctgctgttgt tcgtcgcccc acagcaccag
     3061 cgcattactc accgccgacg gcgccagcac cgcgtaggcc aggcccatgt cgccctgcgc
     3121 cagcgcctcg gtcaccagcg cgttggtcac tgcgttgcgc tcattgccgg cgccgccgag
     3181 ctcctccggc acgttcatca gcgtgaggcc cagctcggcg ctctgctgca gcagcgcggc
     3241 aggcgcggcg cagtcatggt cggcctgcgc cgccacgggg cgcagttcgt tgagcgcgaa
     3301 gttcaagacg gcttcgcgca acgcctgctg ctcgtcggta ggcgtgagat caaacaggtc
     3361 gccggatttt gcccgcgccg gccgcgccgg cttgagcaat ttctgcgcgg cgacaaaact
     3421 gcgtcccacc gtgccggcag tgcggaagcc ggtgcggctg gcgtggtaga ccgcgcgctc
     3481 gctggccttg cgcaggccca ggcgatcgat cagcggcgag ccggcccagc ggttcagcgc
     3541 cttgagcccc gcgcccatca cccgctggta ggcattcatg cgtcttctcc ttccagcgcc
     3601 tgctgggtct tctccaggaa cacgtcgacc aagcgcgtga acttgccctg gatgaacggc
     3661 ggcgccacca gccggtagat cagcggcacc ggcacgtcgc gcagctgccc gttcaccgcg
     3721 aagcgcaggc gcgtgccctt gcgcgcgggc ttgagttcga actcgccgtg gatcacggcg
     3781 ttgccatggc tgggcatgcc ctgccagctg aggcggccgc gcggcgcgtc aacctggtag
     3841 cgcgcggcgt aaaccacctc gtgcgagatg ttggctatgc gcgagccgat cggcgccatc
     3901 tcccacaggt aggtcatgtc gccgagcttc ttcagcttgc gcagcttggg gaagcgccgg
     3961 atggtcgtct cgtagtcttc cagcagcccc gtcacgcgct cgaacggcgc ttccagatcg
     4021 aggctgcgct cgatcgccag atccaccttc atttctcgca cctgtgtcgc taattgttca
     4081 gcgactttgc gccgcgccgg cgcaggcagc tatggcacga gcgacgctgc ggggtgccgc
     4141 aaggccaatc gcctcagaag cggatctgcc cctgcattat ctgccggtgc agtgcctggg
     4201 tcagcggctc gtagccgcgc tcacggtcga gcagcacgta cagcggctcg ccgcaggcct
     4261 gcggatcgat gccctgctgc ttgagttcca ccttgcggaa cttgaaggtg ccggtaactt
     4321 cctgttcctc acgcagccgg agaaacacgg ggaccgcgta ggggggcaat gccaccagca
     4381 ggtgatgggc gagttccgcg cccgggaaat ccgctccctt gtacgtcagc gcggccatgc
     4441 ccgcgcggcc gtcggcgccg ggcagttgta caccgtagac caccgcctca tgtacgccgg
     4501 ggaagccgtt gagcgcaccc tccacttcgg tggtggcgac gttttcgccc ttccagcgga
     4561 aggtatcgcc agtgcggtcg acaaactgga tgtggcggaa accctggtcg cgcaccaggt
     4621 cgccggtatt gaaccagcag tcgccctttt cgaacacatc gcggaacagc ttggcttcgc
     4681 tggccttggc gtcggtgtag ccatcgaagg gattgcgctc ggtgacctcg ctgatcagca
     4741 ggccgacgcc gcctttttcc accttgcgca tgtagccctg cgcatcgcgc gccggcgctt
     4801 cctgatccgc gtcgtattcg acgatggcaa acgtcaaggg gcagaagccc gccgtgcgcg
     4861 acagtccaa
//
LOCUS       DASZZC010000029        11589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1223070,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000029 DASZZC010000000
VERSION     DASZZC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11589
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223070"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VHE37_00690"
     CDS             <1..806
                     /locus_tag="VHE37_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34070.1"
                     /translation="PATIDETDKGFNPKFALTWKPRRDFSIYGSAVRGFRFGGINAVT
                     PPEAAALNVPITFKSDTLWNYELGTRTDWFNGRMTADLTVFYIDWKKPQILQLKAGAI
                     GYTDNVGAARSQGVESQVSALLPWNLFASISGAYIDARTTEEFNSDNGAVPPGTLMPA
                     TPYVSGAGVLTHRLNLAGWQLLSSYTITYHGWSHNTLQNTTPLPAYVLHGLSLNVVKT
                     DWSLRPELNFSVNNLADKHVPLFAEKTPGSPVIIANQPRTIALTLSLSY"
     gene            846..2573
                     /locus_tag="VHE37_00695"
     CDS             846..2573
                     /locus_tag="VHE37_00695"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVT34071.1"
                     /translation="MSKPNSVNLSPAAWPRGEYERWMAAQNVDRTSAGEARGRNGAVT
                     VAYNGLAARAGLEALKQGGTAVDAALTAALAQVALTAGAPISYFGIMSLVYFEAATGK
                     VHTMNAEWNTVRGETEPLTIPGGFDFSSADALRGKGDPSGRTALVGGFMKGVGEAHRR
                     FGRLPFASLFEPSIYICEHGMPVTALVAEQIAFRKNDLARRADTRSIFYKGDGGIYRE
                     GDLFRQPQAAQTLRTVAAQGADYFYTGAWAEKLVREVQAEGGRMTLDDLKDYQVLWSD
                     AQVAELPGGYSLHMPPHPNAGTINLIEALNLAEAAKLADDGPWWKSGTSLRKAVEITS
                     QMMAMFLPDAMLAQIYPGLDLSPQARVTPAHAQELWKRMQAGAKLARFAPAPPRHSDD
                     VVAVDAAGNMAAITHSINTVFWGKTAIFIDGISISDAASFQQAQIAKVQPGERLPAPT
                     ETGVLFKDGKPHLAFASMGAGLHQRSFQGLFNVMRFGMSVPEAINAPDFYMPTTDPKT
                     YTSTLAVPGGRFPKQVLDETGLAWREVPLAEARLGGEGKWVAISRDARTGQLEAASHN
                     RNNSDACAY"
     gene            2593..4011
                     /locus_tag="VHE37_00700"
     CDS             2593..4011
                     /locus_tag="VHE37_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34072.1"
                     /translation="MEPASPQSAAHYTTAVVFGATLLATFIAAWLGRRHSGGSHDGLA
                     TEKLNRWLVGLSAGAAANSGFIVTGAVGLGYTAGLHWLLLPLAWLCGDIVFWSLFPAR
                     LNAYGRASKATTLSQLIVHGTGARAGTLGLVCGVVVLLCLGGYISAQWLAGEKFLAGA
                     FPVGSITALALFAALIVAYTSIGGFRGSVYVDSLQAVIRLGGTTLALAVMYGAVRAQP
                     DAVHANLAQAGAGFTDLLPAGGWPQVAAFVAGFGAAALGFGLGQPHIVSRYLAGASPA
                     ETRSAWWIYMGFVQYTWMAMTLFGVALRGVMPQLADPEAGLSIFFATHAGSIAAGIIV
                     ADVFATISATANALLVAMAQVLACDLAPRLRLRLSLGAGCVLLGALTMALSLLLQGSS
                     VMCVALSSVSMMAAGIAPAVMVKVSGWRYSAESLLATVLTGIGAALAWRIAGFDAAIN
                     EAAPGLLAGLLANAVISRAQEK"
     gene            4322..5041
                     /locus_tag="VHE37_00705"
     CDS             4322..5041
                     /locus_tag="VHE37_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HVT34073.1"
                     /translation="MCSSYTAVTDLAVLGMYFGVARGPDMPLPVFDKTVWPTGLAPII
                     RLNENGRRCAEAGNFGLVPHWAKELVYGRRTYNARSETVARLASFRDAWKRSQRCVVP
                     AERIYEPCYESGRAVRWAVEREDGEPMGIAGIWVDHPHLKRENGEPLLSFAMLTVSGE
                     GHVVFQRLHAPEDEKRMVVILDPGSYDQWLTCSPDEATQFFRQWMEPLRAYPAPLPPR
                     KSGTPKPAADSDGDLFPPVTD"
     gene            complement(5065..5292)
                     /locus_tag="VHE37_00710"
     CDS             complement(5065..5292)
                     /locus_tag="VHE37_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34074.1"
                     /translation="MDLQDEQEIRWRLANGKLPRDISRVGGGSSSGKTCDACGKMIDS
                     GHTDIEVITKTAALALHPQCFHFLSKLLDWV"
     gene            complement(5893..5976)
                     /locus_tag="VHE37_00715"
     tRNA            complement(5893..5976)
                     /locus_tag="VHE37_00715"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            6107..6385
                     /locus_tag="VHE37_00720"
     CDS             6107..6385
                     /locus_tag="VHE37_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVT34075.1"
                     /translation="MIKAFRCKDTEKMFRGERVKRFVNIESVAMRKLAMLNRAEQLDD
                     LRVPPGNRLEALKGTRAGQHSIRVNDQWRVCFHWTPSGPDQVEIVDYH"
     gene            6397..6699
                     /locus_tag="VHE37_00725"
     CDS             6397..6699
                     /locus_tag="VHE37_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HVT34076.1"
                     /translation="MRKVPYPTPGEILLEEFLKPMAMTQYRLAKEIGVPQRRIGEIIA
                     GKRSITADTGLRLSRFFGMSESFWIGLQLDYDREMAKQAMEKTLARIKPWKLRAAA"
     gene            6710..6997
                     /locus_tag="VHE37_00730"
     CDS             6710..6997
                     /locus_tag="VHE37_00730"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVT34077.1"
                     /translation="MALRKRVKSSGNVFLDLGFPPEEAEVLAMRADLMARLRLQIKRN
                     RWTQGSAAEKLGVSQSRISDLVRCKWEKFSLDLLMVLAAKAGLHPQLKMAA"
     gene            7034..9811
                     /locus_tag="VHE37_00735"
     CDS             7034..9811
                     /locus_tag="VHE37_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HVT34078.1"
                     /translation="MNAVEIEQAITDLAEQPFDPANFPYAFLEAFGNKETTIKRLRAG
                     ASNKSDLGGVLQTNNIHILVCEPGQVSKSLKALKDSPATAKAKAKFILATDGIDFEAE
                     ELTGGDPPVACAFKNFPDHFGFFLPLAGITTVRQISENAFDIRATSRLNRLYVELLKD
                     NPEWGKADRRHDMNHFMARLIFCFFAEDTAIFGEKGCFTETVAQMSAKDSSNTHEVIS
                     ALFLAMNTKKEDRPAAKIPRWADDFPYVNGGLFSGSMDVPKFSKIARSYLLHVGGLDW
                     TKINPDIFGSMIQAVAEDEERGELGMHYTSVPNILKVLNPLFLDDLHSKLEEAGDNPR
                     TLLNLRKRIAKIRVFDPACGSGNFLVIAYKAMRELEAEINKRRGEPDRRSEIPLTNFR
                     GIELRDFPAEIARLALVIAEYQCDVLYRGQKLALAEFLPLRKENWITCGNALRLDWLS
                     ICPPTGTGVKLHVDDLFGTPLDQAQIDFENEGGETYVCGNPPYLGSKWQSDEQKEDLK
                     AIFDSRGVAWKSLDYVAGWFMNAATYGQRTSTVAAFVSTNSICQGLQVPLLWPAIFAT
                     GHQIAFAHTSFKWTNLASNNAGVTVAIVGISARPQAVKRLFSMGDDGSVQERLVDHIN
                     AYLVPGRVNSVEQARRPISEVSPMLFGNMPRDGGAFVLDGEERARVLRQHPEVERFIR
                     PYLGSEQMIQGKPRWCLWIEDEDAPYAHQHPYLKAVLDKVRAFRLASAAGSTRDFAAQ
                     PHRFVQIAGTAKRHSLVVAKVSSERRPYLPVDLLDAKTIVSDLLFALYDAPIWNLAVV
                     ASRLHLVWIATVCGKMKTDFRYSNTIGWNTFPLPKLTEKNVNDLTRCAQEILLARERH
                     YPQTIADLYAADAMPADLRRAHARNDETLERIYIGRTFKNDTERLEKLFELYVGMSGR
                     GAAGQRRAKLA"
     gene            9824..10855
                     /locus_tag="VHE37_00740"
     CDS             9824..10855
                     /locus_tag="VHE37_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34079.1"
                     /translation="MNLSTFLHGDANAPTTMSEIRRLHGAIGTTEFLGMYAYATQSGA
                     ALFDLEIGGNFWAVTPSRWLFGIDYGRTQPQALRLMRSRPNVEIRIHDGMYVVDQRGF
                     VPRRDFHAKMSMLMNQENELSGMVVGSGNFSSNGLRKSVEAGAAVLTHGTDEFDATLR
                     PTLVAADLLWEQATPVADILDVYEERWSASFFRGVTENPPIEADHGTTPIFWIEAGYV
                     TKNRGPHRPGNQIDFPRGMSKYFGFNPPPDLPVNSIIGEVVFETPTGAPVANNLRLGN
                     NMMEKISLPIPETHGFDIYDGKILVFQRANGRFVMRALEADDFETAFGDRLAEVRVMG
                     SGRRYGHIT"
     gene            10881..>11589
                     /locus_tag="VHE37_00745"
     CDS             10881..>11589
                     /locus_tag="VHE37_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016254285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HVT34080.1"
                     /translation="MKHSQRRPVPSVSVSYARNGASTRANAHGMRPMQERAYEKRGEQ
                     YLLIKSPPASGKSRALMFVALDKLQNQGLKQAIIVVPEKSIGASFNDEPLSKFGFWAD
                     WCVEPKWNLCNAPGSDNGGKVKSIGSFLQSADKVLVCTHATFRFAVDAYGVKAFDGRL
                     IAVDEFHHVSANPENKLGLHLGQFIARGKTHIVAMTGSYFRGDAEAVLAPSDEAKFDT
                     VTYTYYEQLNGYEYLKQL"
BASE COUNT         2205 a         3602 c         3691 g         2091 t
ORIGIN      
        1 acccggcgac gatcgacgag accgacaagg gcttcaaccc gaaattcgcg ctgacctgga
       61 agccgcgccg cgacttctcc atctacggct cggcggtgcg cggcttccgc ttcggcggca
      121 tcaacgcggt gacgccgccc gaggccgcgg cgctcaatgt gccgatcacc ttcaagtccg
      181 acacgctgtg gaactacgag ctcggcacgc gcaccgactg gttcaacggg cgcatgaccg
      241 cggatctcac ggtgttctac atcgactgga agaaaccgca gatcctgcag ctcaaggccg
      301 gcgcgatcgg ttataccgac aacgtgggtg ccgcgcgcag ccagggcgtg gaaagccaag
      361 tgagcgcgct gctgccgtgg aacctcttcg ccagcatcag cggcgcctac atcgatgcgc
      421 gcaccaccga ggaattcaac tccgacaacg gcgctgtgcc gccgggcacg ctgatgccgg
      481 ccacgccgta cgtgagcggc gccggcgtgc tcacgcaccg gttgaatctt gcgggctggc
      541 agctgctgtc gagctacacc atcacctacc acggctggag ccacaacacg ctgcagaaca
      601 ccacgccgct gccagcgtac gtgctgcacg ggctgtcgct caacgtcgtc aagaccgact
      661 ggagcctgcg tcccgagctc aatttctccg tcaacaacct ggccgacaag catgtgccgc
      721 tattcgcgga aaagacccca ggcagcccgg tgatcatcgc caaccagccg cgcaccatcg
      781 cgctgacgct gagcctgagc tactgaaaga cgacggcgct ccgcattcaa gccaaggaat
      841 catccatgtc gaaaccgaat tccgtgaatc tctcgcccgc tgcctggccg cgcggtgaat
      901 acgagcgctg gatggcggcg cagaacgtgg accgcaccag cgccggcgag gcgcgcgggc
      961 gcaacggcgc ggtgaccgtg gcctacaacg ggctggcggc gcgcgccggg ctggaagcgt
     1021 tgaagcaggg cggcaccgcg gttgacgccg cgctcaccgc cgcactggcg caggtggcgc
     1081 tcaccgccgg tgcgccgatc agctacttcg gcatcatgtc gctggtgtac ttcgaggcgg
     1141 ccaccggcaa ggtgcacacc atgaatgccg agtggaacac ggtgcgcggc gagaccgagc
     1201 cgctgaccat tcccggcggc ttcgacttca gcagcgccga tgcgctgcgc ggcaagggcg
     1261 accccagcgg acgcaccgcg ctggtcggcg gtttcatgaa aggcgtgggc gaagcgcacc
     1321 ggcggttcgg caggctgccc ttcgccagcc tgttcgagcc gtccatctac atctgcgagc
     1381 acggcatgcc ggtgacggcg ctggtggccg agcagatcgc tttccgcaag aatgacctgg
     1441 cgcgccgcgc cgatacgcgc agcatcttct acaagggcga tggcggcata taccgcgagg
     1501 gcgacctgtt ccgccagccg caggccgcgc agaccctgcg cacggtggcg gcgcagggcg
     1561 ccgattattt ctacaccggc gcgtgggcgg agaagctggt gcgcgaggtg caggccgagg
     1621 gcggccgcat gacgctcgac gacctgaagg attaccaggt gttgtggagc gacgcgcagg
     1681 tggccgaact gcctggcggc tacagcctgc acatgccgcc gcatccgaac gccggcacca
     1741 tcaacctgat cgaagcgctc aacctggccg aggcggcgaa gctggccgac gacgggccgt
     1801 ggtggaagtc cggcacctcg ctgcgcaagg cggtggagat cacttcgcag atgatggcga
     1861 tgttcctgcc tgacgcgatg ctggcgcaga tctacccggg cctggatttg tcgccgcagg
     1921 cgcgcgtcac gccggcgcac gcgcaggaac tgtggaagcg catgcaggcg ggtgcgaaac
     1981 tcgcgcgctt cgcacccgcg ccgccgcgcc attccgacga cgtggtcgcg gtggatgccg
     2041 cgggcaacat ggccgccatc acccactcga tcaacaccgt gttctggggc aagaccgcga
     2101 tcttcatcga cggcatctcg atcagcgatg cggcctcgtt ccagcaggcg cagatcgcca
     2161 aggtacagcc gggcgagcgc ctgccggcgc cgaccgaaac cggcgtgctg ttcaaggacg
     2221 gcaagccgca cctggcgttc gcttcgatgg gcgcgggcct gcaccagcgc tcgttccagg
     2281 gcctgttcaa cgtgatgcgc ttcggcatga gcgtgccaga ggcgatcaac gctccggact
     2341 tctacatgcc gaccaccgat ccgaagacct acaccagcac gctggccgtg ccgggcgggc
     2401 gctttccgaa acaggtgctg gatgaaaccg gcctggcctg gcgcgaagtg ccactggcgg
     2461 aagcgcgcct gggcggcgag ggcaagtggg tggcgatcag ccgcgacgcg cgcaccggcc
     2521 agctcgaggc cgcgtcgcac aatcgcaaca acagcgacgc ctgcgcctac tgaggcgcgc
     2581 taatctgcgg gcatggaacc ggcgtcgccg cagagcgctg cgcactacac cacggccgtg
     2641 gtcttcgggg cgacgctgct ggcgacattc atcgccgcct ggctgggccg ccgccattcc
     2701 ggcggcagcc atgacgggct ggcgacggaa aaactcaacc gctggctggt ggggctgtcg
     2761 gccggcgccg ccgccaacag cggcttcatc gtcaccggcg cggtgggcct cggctacacc
     2821 gctggcctgc actggctgct gctgccgctg gcctggctgt gcggcgacat cgtgttctgg
     2881 agcctgtttc ctgcgcgcct caacgcctac gggcgcgcat cgaaagccac cacgctgtcg
     2941 cagctgatcg tgcacggcac cggcgcgcgc gccggcacgc tgggcctggt ctgcggcgtg
     3001 gtggtgctgc tgtgcctggg cggctacatc tcggcgcagt ggctcgccgg ggaaaaattc
     3061 ctggcgggcg cgttcccggt cggcagcatc acggcgctcg cgctgttcgc cgcgctgatc
     3121 gtggcgtaca ccagcatcgg cggcttccgc ggatcggtct atgtcgacag tcttcaggcg
     3181 gtgatcaggc tcggcggcac cacgctggcg ctggcggtga tgtacggcgc cgtgcgcgcg
     3241 cagcccgatg cggtgcacgc caacctggcg caggccggcg cgggcttcac cgatctgctg
     3301 cccgcgggcg gctggccgca ggtggcggcg ttcgtcgccg gcttcggcgc agcggcactc
     3361 ggcttcggtc tgggccagcc gcacatcgtg tcgcgctatc tcgccggcgc gagtcccgcg
     3421 gagacgcgtt ccgcgtggtg gatctacatg ggcttcgtgc aatacacctg gatggcgatg
     3481 acgctgtttg gcgtggcgct gcgtggcgtg atgccgcagc tggccgaccc cgaggccggc
     3541 ctgtcaattt tttttgccac gcacgcgggc agcattgccg ccggcatcat cgtggccgat
     3601 gtattcgcca ccatttcggc cacggccaac gcgctgctgg tggcgatggc gcaggtgctg
     3661 gcctgcgacc tggcgccgcg cctgcggctg cgcctgtcgc tgggcgccgg ctgcgtgctg
     3721 ctcggcgcgc tgaccatggc gctgtcgctg ctgctgcagg gcagcagcgt gatgtgcgtg
     3781 gcgctgtcgt cggtgtcgat gatggccgcg ggcatcgcgc cggcggtgat ggtcaaggtg
     3841 tccggctggc gatatagcgc ggaatcgctt ctggccacgg tgctcaccgg catcggcgcc
     3901 gcgctggcct ggcgcatcgc gggtttcgat gcggcgatca acgaggcggc gccgggattg
     3961 ctcgccgggc tgctcgccaa tgccgtgatc agccgtgcgc aggaaaaata aaaaatacag
     4021 gcggtgttcg tcggattttc agcgcgtagc agtcccaccg gaccgctgat cgagcccgaa
     4081 gcgctcagcg aggtcggccg gatcgtgccg ggacgcatgg ccgcattcgc agctcaggta
     4141 aatccagacc tgcccgacca gtcgatcgag ggtgccgacc gggtatctgg gttcgtggcg
     4201 gcgcatgcgg aaattctgtc gccgtgcgtt gaccgggcgc caccggaaaa ttttctcgct
     4261 gataagttga cgtgcgcgcg aagcacgttc tatttatcca caattgagtc aggggcgcat
     4321 catgtgcagc agctacaccg cggtcacgga cctggccgtg ctgggcatgt acttcggcgt
     4381 ggcgcgaggc ccggacatgc cgctaccggt cttcgacaag accgtatggc cgaccggcct
     4441 ggcgccgatc attcgcctca atgaaaacgg ccggcgatgc gccgaggccg gcaacttcgg
     4501 cctggtgccg cactgggcca aggaactggt ctacggccgg cgcacctaca acgcgcgcag
     4561 tgagactgtc gccaggctgg ccagctttcg ggacgcctgg aagcgcagcc agcgctgcgt
     4621 ggtgccggcc gagcggatct acgagccttg ctatgaatcg ggcagggcgg ttcgctgggc
     4681 ggttgagcgc gaggatggcg agcccatggg cattgccggc atctgggttg atcatccgca
     4741 cctgaagcgg gagaacgggg agccgctgct gtcgttcgcc atgctcaccg tgagtggcga
     4801 agggcatgtg gtgttccagc gcctgcacgc gccggaagac gagaagcgca tggtggtgat
     4861 cctggacccg ggcagctatg accagtggct gacgtgctca cccgatgagg cgacgcagtt
     4921 cttcaggcaa tggatggagc cgttgcgcgc ctacccggca ccgctgcccc cgcgcaagtc
     4981 cgggacgcca aagccggcgg ccgactcaga cggcgatctg tttccgccgg tgacggactg
     5041 aagggtccat cagttcccgg ctggctatac ccagtcgaga agctttgaca aaaaatggaa
     5101 gcactgcgga tgcagcgcca gcgccgcagt cttcgtgatg acttcgatgt cggtgtgacc
     5161 gctatcgatc atcttgccgc acgcatcgca ggtcttcccg gaactgctgc cacccccaac
     5221 ccggctgatg tcccgtggca gcttgccgtt ggccagtcgc cagcgaatct cctgctcgtc
     5281 ttgaaggtcc atgtctccct catggaagtc aaaagtgcgc gaattctgcg gattgggctt
     5341 ctgatttcaa gacattgatc ttatttttct agccataaat atcaggcacc cgtttttggc
     5401 catcgcgcac ggcaatgcgc aaaaaaatgg cccggcggcg tcgggggaga gccgccgggc
     5461 ctgctccggc tggggggagc cggctcgtcc agggagtgag acgaggtcag ttgcgctgac
     5521 gtgcttatag atgatggccg ggggctggaa gttcaagggc tgacggtgtc gagattgcgc
     5581 gagtacgcgc agccgcgctg gtcgcaggcg agaatcaccc atggaccgac cccgtactgt
     5641 ttcccgcccg ccttcgcagt cgagcgaaaa atcccgaaga cggttggagg agatcgtcac
     5701 cggcgtgacc ggccggcaga cgggcccgct cagggcgtga agcgaggtca gcggagacgc
     5761 tgacgggttc tagatgggat caggtttccg agatcaagaa gctcgcgcca agctggctat
     5821 cgaaaacgta accccggcgt aacccccgat cgaaatgcaa attcaaatag tcgtcgcaac
     5881 tagttgatta aatggtggtg atgagtggaa tcgaaccacc gacctcagca ttatgagagc
     5941 gtgattgcag gtcgtttttc atcaacaata tcagccacat gcggcgtgtg cttcggctga
     6001 ctgtgccaat tttgtgccac tgcgcaggcc tttcaggagc ttttgatcga gtgcgctgtg
     6061 cggtactgat ggtttacgtt actaacggaa ctcgttacta ttgcccatga tcaaagcgtt
     6121 caggtgcaaa gacaccgaga agatgtttcg cggcgagcgc gtcaaacgct tcgtcaacat
     6181 cgaatcggtg gcgatgcgca aactggcgat gctgaaccgg gccgagcaac tggacgacct
     6241 gcgggtgccg ccgggcaacc ggctcgaagc tttaaaaggc acgcgcgccg ggcagcacag
     6301 catccgcgtt aacgatcaat ggcgcgtctg tttccactgg acgccaagcg ggccagatca
     6361 agtcgagatc gtggattacc attgaggaaa atggccatgc gcaaagttcc ctacccaacg
     6421 cccggcgaaa ttttgctgga agaattcttg aagcccatgg cgatgacgca gtaccggctg
     6481 gccaaggaaa ttggcgtgcc gcagcgtcgc atcggtgaaa tcatcgcggg caagcgctcg
     6541 atcacggcgg ataccgggct tcgactgtcg cgcttctttg gcatgtcgga gagtttctgg
     6601 attggcctgc agctggatta cgaccgcgaa atggccaagc aggcaatgga aaagaccctg
     6661 gcacgaatca agccgtggaa gttacgagca gcggcctaga cgataagcga tggcactgcg
     6721 taagcgcgtc aaatccagcg gcaacgtgtt tcttgacctg gggtttccgc ccgaggaagc
     6781 cgaggtactt gccatgcggg cggatctgat ggcccgactg cgcctgcaga tcaaacgtaa
     6841 caggtggacg cagggcagtg ctgccgaaaa gctcggtgtg tcgcagtcgc gcatctcaga
     6901 cctagtgcgc tgcaagtggg agaaattcag tctcgacctg ctcatggtgc ttgctgccaa
     6961 ggccggactg catccgcagc tgaagatggc ggcatagccg ccgagctcac gaaattgtta
     7021 gggagcgata tcgttgaacg cggttgagat cgagcaggcc attacggacc tcgcggagca
     7081 gcctttcgac cccgcgaact ttccctatgc cttcctcgaa gctttcggca acaaggagac
     7141 gacgatcaag cgccttcgcg ccggcgcgtc gaataagtcc gacctcggcg gcgttcttca
     7201 gaccaataac attcacattc tggtctgcga accagggcaa gtatcgaagt cgctcaaggc
     7261 gctaaaggac agccccgcta ccgccaaggc caaagccaag ttcatcctgg ctactgacgg
     7321 catagacttt gaagcggagg aattgacagg cggcgatcct cccgtggcat gcgccttcaa
     7381 gaactttcca gaccattttg gcttcttcct gccgctggcg ggcatcacca ccgtccggca
     7441 gatcagcgag aacgcattcg atatccgggc gacgagccgc ctcaaccgac tctacgtcga
     7501 actcttgaag gacaaccccg agtggggcaa ggcggatcgt cgccacgata tgaaccactt
     7561 catggcgcgg ctgatcttct gtttctttgc cgaagataca gccatttttg gcgaaaaggg
     7621 ctgcttcacc gaaactgttg cgcagatgag cgcgaaggac tcgtctaaca ctcatgaggt
     7681 gattagcgcg ctatttttag cgatgaacac gaagaaggaa gaccggcccg ctgcgaagat
     7741 tccgcgttgg gccgacgact ttccttacgt caatggtggc ttgttctccg gcagcatgga
     7801 cgtgccgaaa ttcagcaaga tcgcccggtc ctacctcctg cacgtcggcg gcctagactg
     7861 gacaaagatc aacccggata tcttcgggtc gatgatccag gccgtcgcgg aggacgagga
     7921 gcgcggcgag ctggggatgc actacaccag cgttcccaac attcttaaag tgctcaatcc
     7981 gctgtttctc gacgacctgc attccaagct ggaagaggcg ggcgacaacc cgcgcacctt
     8041 gctcaacctg cgcaagcgca tcgccaagat cagggtcttc gacccggcct gcggctccgg
     8101 caacttcctt gtcatcgcgt acaaggcgat gcgcgagctc gaggctgaga tcaacaagcg
     8161 gcgcggcgag cctgaccgtc gatcggagat accactcacg aactttcgcg ggatcgaatt
     8221 gcgcgacttc ccggcggaga tcgcacggct ggcgctggtc attgccgagt accagtgcga
     8281 cgtgctttat cgcggacaga agctcgcctt ggccgagttc ctaccgctgc gcaaggagaa
     8341 ctggatcacc tgcggcaacg cgctgcggct ggactggttg agcatttgcc cgccgacagg
     8401 aacaggcgtc aagctccatg tcgatgacct gttcggcacg ccgctggacc aggcgcaaat
     8461 cgatttcgag aacgaaggtg gagagaccta tgtctgcggc aacccgccat acctaggcag
     8521 caagtggcag tcggacgaac agaaagaaga cctcaaggcc atcttcgata gccgcggagt
     8581 cgcctggaag tcgctggact acgtcgccgg ttggttcatg aacgcggcca cttacggtca
     8641 gcgcaccagc acggtcgcgg ctttcgtctc cacaaactca atctgccaag gcttgcaggt
     8701 tcccctcctg tggcccgcga tcttcgccac cgggcaccag atcgcgttcg cacatacctc
     8761 gttcaagtgg accaatctcg cgagcaataa cgccggtgtg accgtagcca ttgtgggaat
     8821 atcggcaagg ccgcaagccg tgaagcgctt gttctcgatg ggcgatgacg gttccgtgca
     8881 ggagcggctg gtagatcaca tcaatgccta cctcgtgccg ggccgcgtca actcggtgga
     8941 gcaagcgcgt cggccgattt ccgaggtgtc ccccatgctg ttcggcaaca tgccgcgtga
     9001 cggcggtgcg tttgttctgg acggcgagga acgggcgcgt gtgctgaggc agcaccccga
     9061 agtcgaacgc ttcatccgtc cgtacctggg ctcagagcag atgattcagg gcaagccgcg
     9121 atggtgcctg tggattgagg acgaggatgc accctatgcc caccaacacc cctacctgaa
     9181 ggccgtattg gacaaagtgc gtgcatttcg tctcgcgagc gccgcggggt ccactcgcga
     9241 ttttgcggca cagcctcatc gctttgtcca aatcgcggga accgcaaagc ggcattctct
     9301 agtcgtggcg aaggtctcgt cggagcgacg gccctacctg cccgtggatc tcttggacgc
     9361 taagacgata gtcagtgacc tgctgttcgc cctgtacgac gccccgattt ggaacttggc
     9421 agtggttgcg tcccggctac atctcgtctg gattgccacc gtttgtggga agatgaagac
     9481 ggacttccgc tactcgaaca ccatcggctg gaacacgttc ccgttgccga agttgactga
     9541 gaagaacgta aacgatctca cccggtgcgc tcaggaaatc ttgctagcgc gtgagcgtca
     9601 ctacccgcaa accatcgcag atctctatgc ggctgacgcg atgccggccg atttacgtcg
     9661 cgcgcatgcc cggaacgacg agaccttaga gcgaatctat attggtcgaa cattcaagaa
     9721 cgacacggag cgccttgaga aactgttcga gctgtacgtc ggcatgtccg gtcgaggggc
     9781 cgccggacag cggcgagcca agcttgcgta gcgaggagtg acgttgaacc tatcaacctt
     9841 tctgcatggc gacgcgaacg cgccgaccac aatgtcggag atccggcgcc tccatggtgc
     9901 gatcggcacc accgagttcc tggggatgta cgcatatgcg acccagtccg gcgctgcgtt
     9961 gtttgatttg gagattggcg gcaatttctg ggccgtcacg ccttcgcgat ggctcttcgg
    10021 catagactac ggccgaacgc agccgcaagc ccttcgtctg atgcgctcgc ggccgaacgt
    10081 ggaaatcaga atccacgatg ggatgtatgt cgtggaccaa cgcggctttg ttccccggcg
    10141 cgactttcac gccaagatgt ccatgctgat gaatcaggag aatgagctgt cgggcatggt
    10201 ggttggctct ggaaactttt cgtcgaatgg gcttcgcaag agcgtcgaag cgggtgccgc
    10261 cgtgctcacg cacggcactg atgaattcga tgcgacgctt cgacccacgc tcgtcgctgc
    10321 cgacctcctc tgggaacaag ccacgcccgt tgccgacatc ctcgatgtct acgaggagcg
    10381 gtggagcgcc tctttcttca gaggggtcac cgagaatccg cctatcgaag cggatcatgg
    10441 cacgacaccc attttctgga tcgaagctgg atacgtcacc aagaaccgcg gcccccaccg
    10501 ccccggcaac caaatcgact ttccgcgggg catgtccaag tacttcgggt tcaatcctcc
    10561 gcccgatctg ccagtgaatt cgatcatcgg ggaagtcgtc ttcgaaactc cgactggcgc
    10621 gcccgttgcg aacaacctcc gtctggggaa caacatgatg gagaagattt cgctgccgat
    10681 cccggagacg cacggcttcg acatctacga cggcaagatt ctggtgttcc aacgggcgaa
    10741 cgggcgcttc gtcatgcgcg cgctggaagc agacgacttc gaaactgcct tcggcgaccg
    10801 acttgcagaa gtgcgcgtta tgggcagcgg ccgacgctac ggccatatca cctaacagta
    10861 cggcaaggcg gcacgctcac atgaagcatt cccaacgtcg gcctgttcct tcggtctcag
    10921 tttcctacgc gcgcaacggg gcatcgacca gggccaatgc ccacggcatg aggccgatgc
    10981 aagagcgtgc ctacgagaag cgcggagagc agtacctcct aatcaagtcg ccgcccgcgt
    11041 cgggcaagag ccgcgcactc atgttcgtcg ccctcgacaa gctccagaac caggggctca
    11101 agcaggccat catcgtcgtg ccggagaagt ccatcggcgc tagcttcaac gacgagccac
    11161 tgtcgaaatt cggcttctgg gccgactggt gcgtcgagcc gaagtggaac ctctgcaacg
    11221 cgcccggcag cgacaatggc ggcaaggtga agtcgatcgg ttcgtttctt caaagtgccg
    11281 acaaagtgct ggtctgcacc cacgcgactt ttcgcttcgc ggtcgatgcc tatggcgtga
    11341 aagcgtttga cggccggctg atcgctgtgg acgagttcca ccatgtttcc gccaatccgg
    11401 aaaacaagct tggcctgcac ttggggcagt tcatcgcgcg aggcaagacg cacatcgtcg
    11461 ccatgaccgg ttcttacttt cggggggacg ccgaggctgt gcttgcccca tctgacgaag
    11521 ccaagtttga taccgtcacc tacacctact acgagcaact caacggctac gagtatctca
    11581 agcaactcg
//
LOCUS       DASZZC010000030         7246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_902170,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000030 DASZZC010000000
VERSION     DASZZC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902170"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VHE37_00750"
     CDS             complement(<1..1084)
                     /locus_tag="VHE37_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34081.1"
                     /translation="MPAALLIPASENFPAAAARLILQVHAPPDLSRVTLLVPTPALIR
                     PLRRALARAAGGALLGPQVLTLPAFAQERGAAGSALSPLDARLLLAAALEPHLHLFPQ
                     QDAVALAGELQNLFEQLSAQAQQLALDEAQFRRRVEQGYGGSNGAISREAQIVHLLWR
                     AYLDQTHGASPRVSFQLALKRALAGIADDEQLWLAGFDRLAADEAALLRGALAAKRAC
                     VLLHGRLRGRDCAANTALCQLLHVEPEMLPAQPDARSTWLDAIFAEDAAPLRQRLPRT
                     DAAALPRLQAARSPEHEARMADLAIRRWLLAGKRDIAVVAQDRRYARRLRALLERAQV
                     PLSDEIGWALSTSAAAASLMHWLDVCE"
     gene            complement(1124..2776)
                     /locus_tag="VHE37_00755"
     CDS             complement(1124..2776)
                     /locus_tag="VHE37_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT34082.1"
                     /translation="MSAMKNPVEALLQWAKERPNQPWLHQPVNGVWNTTTWAQAEAQV
                     RSMASALKNLGLPAGSAIAITGRNTAHWFLADLAIGMAGYISVGLYPKQSESAVKYIL
                     NHCEAKVVFIGPMPDEADFFKALPAGVKTIKFPYPDIASKCDHDWDTLVKNSEPYAGY
                     TPPDPKALTTLVYTSGTTGNPKGVMISGENVSFTINGLLKTMPAQGKEIFLSYLPLAH
                     MFERGAVELTSIYLGAEVFFLEVLEKLPETLKQVAPTRFFGVPLVYGRIQSGVLKKMP
                     QEKLDRLLSIPILSSYVKKKIKQGMGLQRARYMFVGAAPMPIPLMQWLEKLGLTVLQG
                     YGMTENSIYATANLPKANRIGSVGRAMPGADMKLSEEGEILFKHPGVMQGYYKEPQKT
                     AETFTADGWLKTGDKGRVDEDGYLYITGRVKDIFKTLKGKYVAPAPIEGALARNTDLD
                     QLCFCGTGLKQPIMVATLNPGSVSKPREAVEKQLAADIDAVNATLEPHEEIAKVLIVK
                     DAWSIDNGMMTPTMKVKRDQVEKKYAALLERESATRSKVAWE"
     gene            3042..4121
                     /locus_tag="VHE37_00760"
     CDS             3042..4121
                     /locus_tag="VHE37_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34083.1"
                     /translation="MRVLKLLLLIIVAGVLYLALAPSPLDPVRWQPPVPPGLEGPYLY
                     NEKLKGIEKLGVGTGTGPEAVVADAQGSIYTGFLDGRVMQFSADGSVAKELVNTHGRP
                     LGVALGEDNTLLIADAFKGLLQLDGKNLSVLSNSAGGVPFGFTDDVDHAAGDDWVYFS
                     DASYKFGFGHHIEDALEHGGNGRLLAYNLKTKETKVLLGGLHFANGIALGPGGEYVLV
                     NLTTEYRVLRYWLKGEKAGTSDVFIDNLPGFPDNITFNGRDTFWLALYAPRDALLDRL
                     LPGPPWVRKLIARLPASLQPKPKMHSFALGLSLDGKVSANLQYRGPDAFAPITSVREH
                     GEYLYFGSLSYPGFGRIALKSVLPD"
     gene            complement(4207..5298)
                     /locus_tag="VHE37_00765"
     CDS             complement(4207..5298)
                     /locus_tag="VHE37_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVT34084.1"
                     /translation="MTNPLLSGIRILDLSRLLPGPFCTLYLAQLGAEVIKIEEPNGGD
                     YARSLSAEMFALINRNKKSITLDLRLPADQEKFHAMVKGADVVLESFRPGVMDRLNCG
                     YEKLRALNPRIVYAALTGYGQDGPYRDRAGHDMNYLGYAGALDQTGTAGGAPAPSNFQ
                     IADLAGGALTCAIGILAAVIGARASGHGTFVDVGMHDGSLALQAVALGTLRTFGKSLP
                     RGADMLTGALPNYSIYQAGDGKYVALGALEPKFFAKFCSEVGRPELAKKPVAPGASAE
                     VLRAEIAAIFRTKTRDEWDALLADKDCCCSGIYAPDELAQNPQVVARRLIEQVGGKPA
                     YAMPIKFSHATLPASTPAPKLGADNKSYL"
     gene            5403..6605
                     /locus_tag="VHE37_00770"
     CDS             5403..6605
                     /locus_tag="VHE37_00770"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HVT34085.1"
                     /translation="MLEHPYASLTALVVDDAKSIRQVLSAMLTDLGVGNVLEAEDGVS
                     AFKVMEQSDKPIDLLFCDLMMPGVDGVETLRGLAARRSDAGVVLLSGMDPKLLMTVAD
                     MGEQLGLRVLGSMAKPFTQQQIKGALESYMRKHRSARAMPMMVTADELDDALRHGRID
                     VYYQPKVRLDTGKLSGVEALARMHHPVYGVIEPDSFIPLAEASTERITQLTMLVLDKA
                     IAQAGIWRAQGLDLGMAINMSTLAIRRLDLPDFVADCARHAQVPHDKITLELTESQVA
                     GGAEILHIVSRFRLRDFKLSIDDYGTGHSGLSRLRRLPFTELKIDRCFVNGAASDGDM
                     RSILETSIDLGRRLRMDVVAEGVETWDDWHLLEGLGCDMAQGYVAARPIPAAGVPVWA
                     QRWSQQVH"
     gene            6777..7100
                     /locus_tag="VHE37_00775"
     CDS             6777..7100
                     /locus_tag="VHE37_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVT34086.1"
                     /translation="MKIKRKMIVLPLMCAAMFQTGHLLAKPARDDAPPRLHVADAELT
                     VHIWDALAQDASLDTHMLEVRAAQGRVQLGGRMLTRTQAARVEDITARVAGVRDVTSL
                     MRVPV"
BASE COUNT         1136 a         2393 c         2449 g         1268 t
ORIGIN      
        1 gttcacacac gtcgagccag tgcatcaggc tggcggccgc ggcgctggtc gacagcgccc
       61 agccgatctc gtcggacagc ggcacctgcg cgcgttcgag cagcgcgcgc agccggcgtg
      121 cgtagcgccg gtcctgcgcc accaccgcga tgtcgcgctt gcccgcgagc agccaccggc
      181 ggatggcgag gtcggccatg cgcgcctcgt gctcgggact gcgcgcggcc tgcaggcgcg
      241 gcagtgccgc ggcatcggtg cgcggcaggc gctggcgcag tggcgcggca tcttcagcga
      301 agatggcatc gagccaggta ctgcgcgcat cgggttgagc aggcagcatc tccggttcca
      361 cgtgcagcaa ctggcacagc gctgtattcg ccgcgcagtc gcgcccgcgc aggcggccgt
      421 gcagcagcac gcacgcgcgc ttggccgcaa gcgcaccgcg cagcagcgcg gcttcatcgg
      481 cggccaggcg gtcgaagccg gccagccata gctgttcgtc gtcggcgatt ccggccagag
      541 cgcgcttcag cgccagctgg aagctcaccc gcggactggc gccgtgcgtc tgatcgagat
      601 aggcgcgcca cagcaggtgc acgatctgcg cctcgcgcga gatggcgccg ttgctgccgc
      661 cgtagccctg ttcgacgcgc cggcggaact gcgcctcgtc cagcgcgagc tgctgcgcct
      721 gcgccgacag ttgttcgaac aggttctgca gctcgccggc cagcgccacc gcgtcttgct
      781 gcggaaacag gtgcaggtgc ggttccagcg cggcagccag cagcaggcgt gcatccagcg
      841 gcgacaacgc ggagccggcc gcgccgcgct cctgggcgaa ggcgggcagc gtgagcacct
      901 gcgggccgag caatgcgcca cccgcggcgc gcgccagtgc acggcgcagc gggcggatca
      961 gcgccggcgt gggcaccagc agggtgacgc gcgacaggtc gggcggcgcg tgcacctgca
     1021 ggatcagccg cgcggcggcg gccggaaagt tttcggaagc gggaatcagc agcgccgcgg
     1081 gcatggcgcc attatcccgc ggcgcggcgg ctggcgcgga gctttactcc cacgccacct
     1141 tgctgcgcgt ggccgactcg cgctcgagca gcgcggcgta tttcttttcc acctggtcgc
     1201 gcttgacctt catggtcggc gtcatcatgc cgttgtcgat gctccacgca tccttgacga
     1261 tgagcacctt ggcgatttcc tcgtgcggct cgagcgtggc gttgaccgcg tcgatgtcgg
     1321 cggccagctg cttctccacc gcctcgcgtg gcttgctcac cgagcccggg ttcagcgtag
     1381 ccaccatgat cggctgcttc aggccggtgc cgcagaagca cagctggtcg agatcggtgt
     1441 tgcgcgccag tgcgccttcg atcggcgccg gcgccacgta cttgcccttg agcgtcttga
     1501 agatgtcctt cacgcggccg gtgatgtaga gatagccgtc ctcgtcgacg cggcccttgt
     1561 cgccggtctt gagccagccg tcggcggtga aggtctccgc ggtcttctgc ggctccttgt
     1621 aatagccctg catcacgccg gggtgcttga acaaaatctc gccttcttcc gacagcttca
     1681 tgtcggcgcc cggcatggcg cggcccaccg agccgatgcg gttggccttg ggcaggttgg
     1741 cggtagcgta gatggagttt tccgtcatgc cgtagccctg cagcacggtc agcccgagtt
     1801 tttccagcca ctgcatcagc gggatcggca tgggcgcggc gccgacgaac atgtagcgcg
     1861 cgcgctgcag gcccatgccc tgcttgatct tcttcttcac gtacgacgac aggatgggaa
     1921 tggacagcag gcggtcgagt ttttcctgcg gcatcttctt cagcacgccg ctctggatgc
     1981 ggccgtagac cagcggcacg ccgaagaagc gcgtgggcgc cacctgcttg agtgtctcgg
     2041 gcagcttctc cagcacttcg aggaagaaca cttccgcgcc gaggtagatg ctggtcagct
     2101 ccaccgcgcc gcgctcgaac atgtgcgcca gcggcaggta cgacagaaag atctccttgc
     2161 cctgcgccgg catggtcttg agcaggccgt tgatggtgaa gctcacgttc tcgccgctga
     2221 tcatcacgcc cttgggattg ccggtggtgc ccgaggtgta gaccagcgtg gtcagcgcct
     2281 tggggtccgg cggcgtgtag ccggcgtagg gctcgctgtt cttcaccagc gtgtcccagt
     2341 cgtgatcgca cttcgaggcg atgtcggggt aggggaactt gatggtcttc acgccggccg
     2401 gcagcgcctt gaagaaatcc gcctcgtcgg gcatcggccc gatgaacacc accttggcct
     2461 cgcagtggtt gaggatgtac ttcaccgcgc tttccgactg cttgggatac aggccgacgc
     2521 tgatgtagcc ggccatgccg atggcgaggt cggccaggaa ccagtgagcg gtgttgcggc
     2581 cggtgatggc aatggcgctg ccggcgggca ggccgaggtt cttcagcgcg ctggccatgg
     2641 aacgcacctg cgcctcggcc tgcgcccagg tggtggtgtt ccacacgcca ttcaccggct
     2701 ggtgcagcca cggctggttg ggccgttcct tggcccactg cagcagcgct tccaccggat
     2761 tcttcatcgc gctcattcgc ttttccccga gtctcgttgt tatcgtggcg cggcagttta
     2821 gcaaacgcgc ttctgcgctc cggcaggtaa aaacggcttt ttttcctgcc accgcaacgc
     2881 catttcgatt gttatcaaat atctgataaa ttaaaaatag taagataatt gataacagcg
     2941 tagatggcgg tgcggtagcg gcaaaaatcg tcctgatttg cccatcgccg acacctgcga
     3001 acacaatcgg cgcatcacaa caatttcaac gggagccttg catgcgcgtg ctcaagctgc
     3061 tgctgctgat catcgtcgcg ggcgtgctct acctggcgtt ggcgccgtcg ccgctggacc
     3121 cggtgcgctg gcagccgccg gtgccaccgg ggctggaagg cccgtatctc tacaacgaga
     3181 agctcaaggg catcgagaag ctcggcgtgg gcaccggcac cgggcccgaa gcggtggtgg
     3241 ccgacgcgca gggcagcatc tacaccggct ttctcgacgg ccgcgtcatg cagttttccg
     3301 ccgacggttc ggtggccaag gaactggtca acacgcatgg ccgcccgctg ggcgtggcgc
     3361 tgggcgagga caacacgctg ctgatcgccg atgcgttcaa gggcctgctg cagctcgacg
     3421 gaaaaaatct ctcggtgctg agcaacagcg cgggcggcgt gccgttcggc ttcaccgacg
     3481 atgtagacca cgccgcgggc gatgactggg tgtacttcag cgacgcctcg tacaagttcg
     3541 gcttcggcca tcacatcgag gacgcgctgg agcacggcgg caacggacgc ctgcttgcct
     3601 acaacctgaa gacgaaggaa accaaggtgc tgctcggcgg gctgcacttc gccaacggca
     3661 tcgcgctcgg cccaggcggc gaatatgtac tggtcaatct caccaccgag taccgcgtgc
     3721 tgcgctactg gctgaaaggg gagaaggccg gcaccagcga tgtgttcatc gacaacctgc
     3781 cgggcttccc cgacaacatc accttcaacg gccgcgacac cttctggctg gcgctgtatg
     3841 cgccgcgcga tgcgctgctc gaccgcctgc tgcccggccc gccgtgggtg cgcaaattga
     3901 tcgcgcgcct gccggcctcg ctgcagccca agccgaagat gcattcgttc gcgctcggcc
     3961 tgtcgctcga cggcaaggtc agcgccaacc tgcagtaccg cggccccgat gcgttcgcgc
     4021 cgatcaccag cgtgcgcgag cacggcgaat acctgtactt cggcagcctg agttatcccg
     4081 gcttcgggcg cattgcgctc aagagcgtgt tgccggattg atcgacaaac gagtgcgaga
     4141 cactgcggcc gtcgtaggag cgggccttgc ccgcgatcag gccgttatcg cgcgcaaggc
     4201 gcgctcctac aggtagctct tgttgtccgc gcccagcttc ggcgccggcg tcgaagccgg
     4261 cagcgtggca tgcgagaact tgatcggcat ggcgtacgcc ggcttgccgc ccacctgctc
     4321 gatcagcctg cgcgcgacca cctgcgggtt ctgcgccagt tcgtcgggcg cgtagatgcc
     4381 ggagcagcag cagtccttgt cggccagcag cgcatcccac tcgtcgcgcg tcttcgttct
     4441 gaagatcgcc gcgatttccg cacgcagcac ttcggcgctg gcgccgggcg ccaccggttt
     4501 cttcgccagt tccggccgcc cgacctcgga gcagaacttg gcgaagaact tgggttcgag
     4561 cgcgcccagc gccacgtact tgccgtcgcc ggcctggtag atggagtagt tgggcagcgc
     4621 gccggtgagc atgtcggcgc cgcgcggcag cgacttgccg aacgtgcgca gcgtgcccag
     4681 cgccaccgcc tgcagcgcca gcgagccgtc gtgcatgccg acgtcgacga aggtgccgtg
     4741 gcccgaggcg cgcgcgccga tcaccgcggc gaggatgccg atggcgcagg tcagcgcgcc
     4801 gccggcaagg tcggcaatct ggaaattgct cggcgccggc gcgccgccgg cggtgccggt
     4861 ctggtcgagc gcgccggcgt agcccaggta gttcatgtcg tggccggcgc ggtcgcggta
     4921 cgggccgtcc tgcccgtaac cggtgagcgc ggcgtagacg atgcgcgggt tgagcgcgcg
     4981 cagtttttcg tagccgcagt tcaggcggtc catcacgccg gggcggaacg actccagcac
     5041 cacgtcggcg cctttcacca tggcgtggaa tttttcctgg tcggccggca ggcgcagatc
     5101 gagggtgatc gatttcttgt tgcggttgat cagcgcgaac atctcggccg acagcgagcg
     5161 cgcgtagtcg ccgccgttgg gctcctcgat cttgatcacc tccgcaccca gctgtgccag
     5221 atacagcgtg cagaacggcc cgggcagcag gcgcgacagg tcaaggatgc ggatgccgga
     5281 cagcagcgga ttggtcatgg tggacaagta tctggctctg ttgtgtttta agtgctcaag
     5341 tcaacgcctg gtgcgccgct aacgatgcag ggccattgtc gcctgcgttt gcggagactg
     5401 ccatgctcga acacccgtat gcctcgctca ccgccctggt ggtggatgat gcgaagtcga
     5461 tccgccaggt gctgtcggcc atgctcaccg acctgggcgt cggcaacgtg ctggaggccg
     5521 aggatggcgt gtcggctttc aaggtgatgg agcagtcgga caagccgatc gacctgctgt
     5581 tctgtgacct gatgatgccg ggcgtcgatg gcgtggaaac cctgcgcggc ctggccgcgc
     5641 ggcgcagcga cgccggcgtg gtgctgctct ccggcatgga tccaaaactg ctgatgacgg
     5701 tggccgacat gggcgagcag ctgggcctgc gcgtgctcgg cagcatggcc aagccgttca
     5761 cccagcagca gatcaagggc gcgctggagt cgtacatgcg caagcaccgg tccgcgcgcg
     5821 ccatgccgat gatggtcacc gccgacgaac tcgacgacgc gctgcgccat gggcgcatcg
     5881 acgtctacta ccagcccaag gtgcggctcg acaccggcaa gctgtccggc gtggaggcgc
     5941 tggcgcgcat gcatcacccg gtgtacggcg tgatcgagcc cgactcgttc atcccgctgg
     6001 cggaggcgtc caccgagcgc atcacgcaat tgaccatgct ggtgctggac aaggccattg
     6061 cgcaggccgg catctggcgc gcgcagggcc tggacctggg catggccatc aacatgtcga
     6121 cgctggccat ccgccggctc gacctgccgg acttcgtcgc cgactgcgcg cgccacgcgc
     6181 aggtgccgca cgacaagatc acgctggagc tgaccgagag ccaggtggcc ggcggcgccg
     6241 agatcctgca catcgtctcg cgcttccgcc tgcgcgactt caagctgtcg atcgacgatt
     6301 acggcaccgg ccattccggc ctgtcgcgcc tgcgccggct gccgttcacc gagctgaaga
     6361 tcgaccgctg ctttgtcaac ggcgcggcca gcgacggcga catgcgctcg atcctcgaaa
     6421 ccagcatcga cctgggccgg cgcctgcgca tggacgtggt ggccgaaggc gtcgagacct
     6481 gggacgactg gcacctgctc gaaggcctgg gctgcgacat ggcgcagggc tacgtcgctg
     6541 cgcggccgat ccccgccgcc ggcgtgccgg tgtgggcgca gcgctggtcg cagcaggtgc
     6601 actgagcgcg ccgccgcggc gcggatgagc ggtcgccgtt cccgcgcgaa cggtcatctg
     6661 ggaattgcat cacgctgctg cgtcgcaccg gcgcagccgc ccgtctgcgt cgacggtcgt
     6721 gcatggcatg cgcgcgctaa cttcagcgcg tgttccgctt gcgtcgggat gccgccatga
     6781 agatcaagag aaagatgatc gtgctgccgc tgatgtgcgc ggcgatgttc cagaccgggc
     6841 acctgctggc caagcccgcg cgcgacgatg cgccgccgcg gctgcacgtg gccgacgccg
     6901 aactcaccgt gcacatctgg gacgcgctgg cgcaggatgc atcgctcgac acccacatgc
     6961 tcgaagtgcg cgccgcgcag ggacgcgtgc aactgggcgg ccgcatgctc acgcgcacgc
     7021 aggccgcgcg cgtggaggac atcaccgcgc gcgtcgcggg cgtgcgcgac gtcaccagcc
     7081 tgatgcgggt gccggtctag cctccaccgc acctgccttg cgcgtgttat taattattaa
     7141 tttattttaa aatatataga taattaataa catcgaaatc cggggtgcgg tgagctcgtt
     7201 tccgggtctc atccggcctc ctgcatcgtt gcagcagcgc gccttg
//
LOCUS       DASZZC010000031         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_579535,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000031 DASZZC010000000
VERSION     DASZZC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579535"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /gene="tilS"
                     /locus_tag="VHE37_00780"
     CDS             complement(<1..497)
                     /gene="tilS"
                     /locus_tag="VHE37_00780"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HVT34087.1"
                     /translation="MKKALDLKLPRGARVWLAYSGGVDSTMLLHLLASKKLPRFAAIH
                     VHHGLQPQADAWARGCEKQCAALGVKCLVVRVQVNADDPAGPEAAARKARYAALLAQM
                     GKDDLMLTAHHQDDQAETVLLRLLRGSGVEGLAAMRAVTPFGPGQLVRPLLDVPAEAI
                     RDYARA"
     gene            complement(635..1597)
                     /locus_tag="VHE37_00785"
     CDS             complement(635..1597)
                     /locus_tag="VHE37_00785"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HVT34088.1"
                     /translation="MNLNYLDFEQPIAELQQKIEELRFATSGSGVNLTEEISRLEQKS
                     RQLTEDIFSRLTPWQVAQLARHPQRPYALDYIKTLFTEWEELHGDRNLDDDAAIVGGL
                     ARLDGRACLVIGQQKGRDAKERVFRNFGMPKPEGYRKALRLMKLAEKFNLPILTFIDT
                     PGAYPGISAEERNQSEAIARNLLELSKLRTPVLATVIGEGGSGGALAIGVADHVMMLQ
                     YSIYSVISPEGCASILFKNAGRAPEAAEAMRVTASDLYEQRLIDEVIPEPLGGAHRDS
                     AAMMNTLKQKLTTNLERLRHTPMTKLLTTRYQRLMSYGAYETVH"
BASE COUNT          242 a          534 c          518 g          310 t
ORIGIN      
        1 gcgcgcgcgt aatcgcggat ggcctcggcc ggcacgtcga gcagcggccg caccagctgg
       61 ccggggccga acggcgtcac cgcgcgcatt gccgccagtc cttccacgcc gctgccgcgc
      121 agcaggcgca gcagcacggt ctcggcctgg tcgtcctggt gatgcgcggt gagcatcagg
      181 tcgtccttgc ccatctgcgc cagcagcgcc gcgtagcgcg ccttgcgcgc ggcggcttcc
      241 gggccggcag gatcgtcagc gttcacctgc acgcgcacca ccaggcactt cacgcccagc
      301 gccgcgcatt gcttctcgca gccgcgcgcc cacgcatccg cctgcggctg caggccatgg
      361 tgcacgtgga tcgccgcgaa gcgcggcagc ttcttcgatg ccagcaggtg cagcagcatg
      421 gtggagtcca cgccgccgga atacgccagc cacacgcgcg caccgcgcgg cagcttgaga
      481 tcgagcgcct tcttcatgcc gccacccttc ccttgatgac gattttcatg ctcaccgcac
      541 cccggattac gttgttatta attattgagt gattttaatt tcactataca atttataaca
      601 tcaaaaatac cggtgcggtg gactgttttt cgcctcagtg cacggtctcg taagcaccgt
      661 agctcatcag gcgctgatag cgcgtggtca gcagcttggt catcggcgtg tgccgcaggc
      721 gctcaagatt cgtggtgagc ttctgcttga gcgtgttcat catcgccgcg ctgtcgcggt
      781 gcgcgccgcc cagcggctcg gggatcacct cgtcgatcag ccgctgctcg tacaggtcgc
      841 tcgcggtgac gcgcatggcc tcggcggctt ccggcgcgcg accggcgttc ttgaacagga
      901 tggaggcgca gccttccggc gagatcaccg agtagatgct gtactggagc atcatcacgt
      961 ggtcggcgac tccgatcgcc agcgcgccgc cggagccgcc ttcgccgatc accgtggcca
     1021 gcaccggtgt gcgcagcttg gacagttcca gcaggttgcg cgcgatggct tccgactggt
     1081 tgcgctcctc ggcagagatg ccgggatacg cgcccggcgt gtcgatgaag gtgaggatcg
     1141 gcaggttgaa tttctcggcg agcttcatca gccgcagcgc cttgcggtag ccctcgggct
     1201 tgggcatgcc gaagttgcgg aacacgcgct ccttggcatc gcgccccttc tgctggccga
     1261 tgaccaggca ggcgcggccg tccaggcgcg cgagtccgcc gacgatggcg gcgtcgtcat
     1321 cgagattacg gtcgccgtgc agctcttccc actcggtgaa cagcgtcttg atgtagtcca
     1381 gcgcataggg ccgctgcgga tggcgcgcca gctgcgccac ctgccacggc gtcagccgcg
     1441 agaagatgtc ctcggtcagc tggcggctct tctgctccag ccgcgagatt tcctcggtga
     1501 ggttgacgcc gctgccgctg gtggcgaagc gcagctcctc gatcttctgc tgcagctcgg
     1561 cgatgggttg ttcgaaatcc aggtagttca ggttcatggc atca
//
LOCUS       DASZZC010000032         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_452954,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000032 DASZZC010000000
VERSION     DASZZC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452954"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VHE37_00790"
     CDS             complement(<1..258)
                     /locus_tag="VHE37_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34089.1"
                     /translation="MSVAKSSAILAMTCVLLLAGGCNRDATDKNAADVQKAEEAGARK
                     VEQAQVDASRNVAGANKDVQNAQNELAHETAEGREHVALAEA"
     gene            complement(378..1160)
                     /locus_tag="VHE37_00795"
     CDS             complement(378..1160)
                     /locus_tag="VHE37_00795"
                     /inference="COORDINATES: protein motif:HMM:NF020455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CVNH domain-containing protein"
                     /protein_id="HVT34090.1"
                     /translation="MLRLGGLILLLGMGVTQVAHADPYISCSEMADYANDAAELSDMI
                     QRNRRLDFNSDRELNDTIRDLATAMLQLAKAEKNQNFIQAARDMKQAWRNSDEQAYAG
                     ALDRAADLLAAMRTLRCPNDAARPHAYSHDGALAVPRGPYQNSCEEIRMTGDGRLHAS
                     CRKRNGHDTVRSYIEPRRCSTEILNENGALVCDGYHPKLPTGPYADQCRDLRMDGYDL
                     SASCLNNRGRRVDTVMTEIFHCLPGTMRDDNGLLACDVKEND"
     gene            1344..1715
                     /locus_tag="VHE37_00800"
     CDS             1344..1715
                     /locus_tag="VHE37_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34091.1"
                     /translation="MKSMLHSMRLVAGATVAAVAMAGTASAGDQDFTLVNKTGVEIHR
                     MYTSPHSTTDWEEDVLGQGTLADGESLHVAFSPKEKAAKWDLKVEDADGNSITWENLN
                     LMEISKITLHYDKGNATADLE"
     gene            complement(1783..>2660)
                     /locus_tag="VHE37_00805"
     CDS             complement(1783..>2660)
                     /locus_tag="VHE37_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34092.1"
                     /translation="TSVATAYDETVYVTDFDNNAIRAISYYDGSVSTLVHMTGFSRPF
                     GIVYSYVDDNLYVETDDDDTGAHSGTTGTIWKINRYTGVATVVARDIGRPRGLEVLSD
                     GRLALSDLTHQNISILDPVTGNLTLLAGSTTDAAGYTDATGSNALFNRPYGLALASNG
                     DLLVADQNNNVIREVTLAGVVTTYAGTGTVGSANGPRATATFSGPEDVAVFNDVVYVS
                     DQGNHLVRVIFNDRVSVQAGDGNAGFFDGNSTDAEFFGMEGIDTSPGGHLLWIADGNV
                     GNGGPYNRVRFIWVS"
BASE COUNT          432 a          860 c          854 g          514 t
ORIGIN      
        1 ggcctcggcc agcgccacgt gctcccggcc ctcggccgtt tcgtgcgcca gttcgttctg
       61 cgcgttctgc acgtccttgt tcgcgccagc gacattgcgc gacgcatcga cctgcgcctg
      121 ttcaaccttg cgcgcgccgg cctcctcagc cttctgcacg tcggcagcat tcttgtcggt
      181 ggcatcgcgg ttgcagccgc ctgccagcag cagcacgcag gtcatggcca ggatcgctga
      241 cgatttcgct acggacatgg aactctctcc gatggtttcc tcgggtgttt agaccgaaga
      301 accccggcta ccgtccagtc ttccgccgag aaggaagcac ggaacttttt ccgcggaccc
      361 tgcgcaacag ttcccgctca gtcgttttcc ttcacgtcgc aggccagcaa gccgttgtcg
      421 tcacgcatgg tgccaggcag acagtggaag atctcggtca tcaccgtatc gacgcgccgc
      481 ccacgattgt tcaggcagga agcggagaga tcgtaaccgt ccatgcgcag gtcccggcat
      541 tgatcggcgt acggtccggt gggcaacttg ggatgatagc catcgcacac cagcgcgccg
      601 ttctcgttga gaatctcggt gctgcagcgg cggggttcga tatagctgcg cacagtgtcg
      661 tgaccgtttc gcttgcggca gctggcgtgc agccggccat cgccggtcat cctgatttcc
      721 tcgcagctgt tctgataggg cccacgcggc actgcgagcg cgccgtcgtg ggaatacgca
      781 tgtggacgcg ccgcatcgtt cggacagcgc agcgtgcgca tcgccgccag caggtcggcg
      841 gcgcggtcaa gcgcgccggc ataggcctgt tcgtcggaat tcctccaggc ctgcttcatg
      901 tcgcgcgcgg cctggatgaa gttctggttc ttctcggcct tggccagctg cagcatggcc
      961 gtggcgagat cgcggatggt gtcgttcagc tcgcggtcgg agttgaagtc cagcctgcgg
     1021 ttgcgctgga tcatgtccga caattctgcc gcgtcattgg cgtagtccgc catttcgctg
     1081 cagctgatgt acgggtccgc gtgcgccacc tgcgtcaccc ccatgcccag cagcaggatc
     1141 aatccgccaa gtcgcaacat gaattccccc gacaatgttg tacggccgcg gcttgttccc
     1201 ccgaactccg cgctgccgcc ccaagcctac cccaagcgcc accacggtgc gcgcatggga
     1261 caacacttgc tattgcgcga atgcgccatg cacccgtgat agtctccaac agcccgctct
     1321 tgggcatgtt cgggggaaca gagatgaaat cgatgctgca ttccatgcgg ctggtggccg
     1381 gggccaccgt cgctgccgta gccatggctg gcaccgcctc tgcgggcgac caggacttca
     1441 ccctggtcaa caagaccggc gtggaaatcc accgcatgta cacgtcgccg cacagcacca
     1501 ccgactggga agaggacgtg ctcggccagg gcacgctcgc cgacggcgag agcctgcacg
     1561 tggcgttcag cccgaaggag aaggcagcca agtgggatct caaggtggag gatgccgacg
     1621 gcaattccat cacctgggaa aacctgaacc tgatggagat ctcgaagatc accctgcact
     1681 acgacaaggg caatgcgacg gccgatctcg agtagagtgc cgctgtaacg acaaagccgc
     1741 tggccttacc gccagcggct tttttcgtgg cgcattcctg tttcagctga cccagatgaa
     1801 tctcacgcgg ttgtacggcc cgccgttgcc gacattgccg tcggcgatcc acagcaggtg
     1861 gccgcccggg ctggtgtcga tgccttccat gccgaagaac tcggcgtcgg tgctgttgcc
     1921 gtcgaagaac ccggcgttgc cgtcgccggc ctgcaccgag acgcggtcgt tgaagattac
     1981 gcgcaccagg tgattgccct ggtccgacac gtagaccacg tcgttgaaca ccgccacgtc
     2041 ttccggcccg ctgaatgtcg ccgtggcgcg cggcccgttg gccgaaccga cagtgccggt
     2101 gccggcatag gtcgtcacca cgccggccag cgtcacctcg cggatcacgt tgttgttctg
     2161 gtcggcgacc agcaggtcac cgttgctggc cagcgccagc ccatacggcc ggttgaacag
     2221 cgcattgctg ccggtggcat cggtgtagcc ggcggcgtcg gtggtgctgc ccgcgagcag
     2281 cgtgaggttg ccggtgaccg ggtcgaggat ggagatgttc tggtgcgtga ggtccgacag
     2341 cgccaggcgg ccgtcactca gcacttcgag tccgcgcggg cggccgatgt cgcgcgccac
     2401 caccgtcgcc acgccggtgt agcggttgat cttccagatg gtgccggtgg tgccgctgtg
     2461 cgcgccggtg tcgtcgtcgt cggtctccac gtagaggttg tcgtcgacgt agctgtagac
     2521 gatgccgaac ggccggctga agccggtcat gtgcaccagc gtgctgacgc tgccgtcgta
     2581 gtacgagatg gcgcggatgg cgttgttgtc gaaatcggtc acgtagacgg tttcgtcgta
     2641 ggccgtggcc acgctggtgg
//
LOCUS       DASZZC010000033         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_131762,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000033 DASZZC010000000
VERSION     DASZZC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131762"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VHE37_00810"
     CDS             <1..419
                     /locus_tag="VHE37_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HVT34093.1"
                     /translation="ACSFALGRALESTGAAQLLAHGMLALPGDNPWMVLTLAYGATML
                     VTELITHNAAAVLMYPICMAAAKALGVSQLPFVMAVTMAASASFSTPLGYQTNMMVFG
                     PGGYRFTDFLKVGIPLQLLLWAVSAFVIPMVWPFRP"
     gene            complement(426..1349)
                     /locus_tag="VHE37_00815"
     CDS             complement(426..1349)
                     /locus_tag="VHE37_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="This protein contains a GlyGly-CTERM
                     protein-sorting domain, as detected by TIGR03501. These
                     domains are found at the C-terminus of secreted proteins
                     in organisms that possess both rhombosortase, which is an
                     intramembrane serine proteinase (see TIGR03902), and a
                     type II secretion system (T2SS). In at least some cases,
                     such as VesB from Vibrio cholerae, cleavage by
                     rhombosortase is followed first by attachment of a
                     glycerophosphoethanolamine-containing moiety, then by
                     transport by the T2SS across the outer membrane and
                     release into the medium in soluble form; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlyGly-CTERM sorting domain-containing protein"
                     /protein_id="HVT34094.1"
                     /translation="MMTSIQVLRHGPWRLLCAVILLVSMMPARASIDFSTGSLYRSAG
                     VPAWSPASGVFTLMLWAYLPNPTGINDFFKADDGGPRQVWANIGTASDSPTEPYFYTA
                     NGEWHFNKDGTFVANPPQSFDVSAGWTFLAVSFTQGGALDLYAWQGGVNGGQLVHIPV
                     TATGASIGGSGTINTFNLGAESGYGEGCTCLVGPVYIYDGVAMTQAQIDAQRQQLDPV
                     TSERLIEFSTFTDPARLAVDESGTADWTKHGTVQYNNSNPAVAGGTSGTGGSSSGGGA
                     GRFGGAGSSGGAMNLVSMLALGLAAWLRRRG"
     gene            complement(1413..1994)
                     /locus_tag="VHE37_00820"
     CDS             complement(1413..1994)
                     /locus_tag="VHE37_00820"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT34095.1"
                     /translation="MSLRHAILVLLQDHPGSGYDLVSRFRSGIGHFWNATHQQVYQEL
                     KQLSRERLVQHEVHEQDEAPDKKVYQVTRAGRQALGDWYQQVVKPPKLRDALLVKVYG
                     AGPKQAPGLIQELEQHRLLQQKALAEYQSLEAHYFAQDRATRERFRQPYLTLRRGIRY
                     VQGTLEWIDEAIDWLRGGKLPTRPLLPVRPSKG"
     gene            2081..3727
                     /locus_tag="VHE37_00825"
     CDS             2081..3727
                     /locus_tag="VHE37_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT34096.1"
                     /translation="MERIGRRYRSGRAAAHYDAIVVGSGIGGLTTAALLSKLGRKVCV
                     LEQHYTAGGFTHSYERGGYEWDVGVHYVGDVGKPTSQLRRVFDVITEGALQWAPMDAC
                     YDRIMVGGRRYDFVAGRDNFAAELKKHFPDEAQAIDRYIELVYRVARSSPRFFAGQAM
                     PPRVARIYNKLRRHLVPRECLMTVRQVLESLTQNQELIGVLAGQWGDYGMVPEQASFI
                     MHAAVAKHYFNGGFYPAGGSWKIAQHVLPVIRASGGEVFTYARVKRIVAEHGRACGVE
                     LDNGDVLRGRQIVSSVGARMTFGELIEPALRQQLGYETPLRQARPSGSSLCLFLGFKG
                     SAAELDLPKTNLWIYPGPHHEQNVARFATDRNADFPLLYVSFPSAKDPEWDRHYPGKS
                     TVQVITMAPYEWFAQWQGSTWNQRGADYEALKQQFTERMLAALYREMPQLQGRLDIAE
                     LATPLSTQWFHLYDRGEIYGLDHDAQRFSDDWLQSTTRLAGLYLTGQDVVTAGVGGAM
                     MAGVLTTAAMLGLQSRKVWKLLKQWAPAAAHNGATAPVSA"
     gene            complement(3738..4337)
                     /locus_tag="VHE37_00830"
     CDS             complement(3738..4337)
                     /locus_tag="VHE37_00830"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HVT34097.1"
                     /translation="MKKNPQVDRYIRDAAPYAQPILRHLRKLVHQACPQAEEKMKWSF
                     PHFDYRGMMCSMAAFKQHCAFGFWKHKLLLAEGSALNRDAMGSFGRITGLDDLPPDAQ
                     LLELIRRAMALNDSGTKVEKPKAARKPPPQAPADLAAALKKNAKARTTYEAFSNTNRR
                     DYVEWITEAKTEETRQRRLLQAIEWMAEGKPRNWKYQKC"
     gene            complement(4384..>4705)
                     /locus_tag="VHE37_00835"
     CDS             complement(4384..>4705)
                     /locus_tag="VHE37_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34098.1"
                     /translation="ARAIFGSRVAWDFNLRDYYISGIASTETGGSENIARADTSITWR
                     IWGLHGVTLKYALSQREAQYPDNPAQRQRVGTVSIAYTLLGSTRFGAVDWRPSAMYKP
                     PDKM"
BASE COUNT          716 a         1594 c         1579 g          816 t
ORIGIN      
        1 tcgcctgctc gttcgcgctc ggccgtgcgc tggagagcac cggcgccgcg cagctgctgg
       61 cgcatggcat gctggcattg cccggcgaca atccatggat ggtgctgacg ctggcctacg
      121 gcgcgaccat gctggtcacc gagctgatca cgcacaatgc cgcggcggtg ctgatgtacc
      181 cgatctgcat ggcggcggcc aaggcgctgg gcgtgagcca gctgcccttc gtgatggcgg
      241 tgaccatggc ggcgtcggcc agcttctcca cgccgctggg ctaccagacc aacatgatgg
      301 tgttcgggcc cggcggctac cgcttcaccg atttcctcaa ggtgggcatc ccgctgcagc
      361 tgctgctgtg ggcggtgagc gctttcgtca tcccgatggt gtggccgttc cggccctgac
      421 cgcgatcaac cgcggcggcg cagccacgcg gccaggccca gcgccagcat cgagacgagg
      481 ttcatcgcgc caccggaact gccggcgccg ccgaacctgc cggcgccgcc gccgctgctg
      541 ctaccaccgg tgcccgatgt tccgcccgcg accgccggat tcgagttgtt gtattgcacg
      601 gtgccgtgct tggtccaatc cgcggtgccg ctctcgtcca ccgcaagccg ggctgggtcg
      661 gtgaaagtgg aaaactcgat cagccgctcg ctggtgacgg gatcgagctg ctggcgctgc
      721 gcgtcgatct gcgcctgcgt catggccacg ccgtcataga tgtagaccgg accgaccagg
      781 caggtgcaac cctcgccgta gccgctctcg gcgccaagat tgaaggtgtt gatggtgccg
      841 ctgccaccga tgctggcacc tgtcgccgtc accgggatat gcaccagctg gccgccattg
      901 acgccgccct gccacgcgta caggtccagc gcgccaccct gcgtgaaact caccgcgagg
      961 aaggtccagc cggcactgac gtcgaaactc tgtggtggat tggcgacgaa ggtgccgtcc
     1021 ttgttgaagt gccactcacc gttggcggtg tagaaatacg gctcggtagg actgtcgctg
     1081 gccgtgccga tgttggccca cacctggcgc gggccgccgt cgtcggcctt gaagaaatcg
     1141 ttgatgccgg tcggattggg cagataggcc cacagcatca gcgtgaagac cccgctggcc
     1201 gggctccacg ccggcacgcc ggcgctgcgg tagagactgc cggtggagaa atcgatcgac
     1261 gcccgtgcgg gcatcatcga gaccagcagg attactgcgc acagcaaccg ccacggcccg
     1321 tgccgaagga cctggatcga agtcatcatc tgccgctgcc ggtggtgaac gacacctgct
     1381 tagaggcacc gcgtgctcag cagttcggtc cgttaccctt tgctcggccg gactggcagc
     1441 agggggcgcg tgggcaattt gcctccgcgc agccagtcga tcgcctcgtc gatccattcc
     1501 agcgtgccct gcacgtagcg gatgccgcgg cgcagcgtca ggtacggctg gcggaagcgc
     1561 tcgcgcgtgg cgcggtcctg cgcgaagtaa tgtgcttcca gactctggta ctcggccagc
     1621 gccttctgct ggagcaggcg atgctgctcc agttcctgga tcagccccgg cgcctgcttc
     1681 ggcccggcgc cgtagacctt caccagcagc gcgtcgcgca acttgggcgg cttgaccacc
     1741 tgctggtacc agtcgcccag cgcctgccgg cccgcacgcg tgacctggta caccttcttg
     1801 tccggcgcct cgtcctgttc gtgcacctcg tgttggacca ggcgctcgcg cgacagctgc
     1861 ttgagctcct ggtagacctg ctggtgggtg gcgttccaga aatggccgat gcccgagcgg
     1921 aagcgcgaca ccaggtcgta gccgctgccc ggatggtcct gcagcagcac caggatggcg
     1981 tgcctgagcg acatgcaatg aactcctgtg actatgcaaa ctattgcata ggatgccgcc
     2041 gcgtgctcta ctatgcaaac atttgcatag gtgaagcgcc atggaacgca tcggacgccg
     2101 ttatcgcagc gggcgcgcag ccgcccacta cgacgccatc gtcgtcggct cgggcatcgg
     2161 cggcctgacc accgccgccc tgctgtcgaa gctcggccgc aaggtgtgcg tgctggaaca
     2221 gcactacacc gccggcggct tcacccacag ctacgagcgc ggcggctacg agtgggacgt
     2281 gggcgtgcac tacgtcggcg acgtcggcaa gccgacctcg cagctgcgcc gggtgttcga
     2341 cgtcatcacc gaaggcgcgc tgcagtgggc gccgatggac gcctgctacg accgcatcat
     2401 ggtcggcggg cggcgctacg atttcgtcgc cggccgcgac aacttcgccg ccgagctgaa
     2461 gaagcatttc cccgatgagg cgcaggccat cgaccgctac atcgagctgg tctaccgcgt
     2521 ggcgcgctcg tcgccgcgct tcttcgccgg ccaggccatg ccgccgcgcg tggcgcgcat
     2581 ctacaacaag ctgcgccggc acctggtgcc gcgcgaatgc ctgatgacgg tacgccaggt
     2641 gctggaatcg ctgacgcaga accaggagct gatcggcgtg ctcgccggcc agtggggcga
     2701 ctacggcatg gtgccggagc aggccagctt catcatgcac gcggcggtgg ccaagcatta
     2761 tttcaacggc ggcttctacc cggccggcgg ctcgtggaag atcgcgcagc acgtgctgcc
     2821 ggtgatccgc gccagcggcg gcgaggtgtt cacctacgcg cgggtcaagc gcatcgtcgc
     2881 cgaacacggc cgcgcgtgcg gcgtggaact ggacaacggc gacgtgctgc gcggcaggca
     2941 gatcgtcagc tcggttggcg cccgcatgac cttcggcgaa ctgatcgagc ccgcactgcg
     3001 ccagcagctg ggctacgaaa caccgctgcg gcaggcgcgc ccttccggct cgtcgctgtg
     3061 cctgttcctc ggcttcaagg ggtcggcggc cgaactggac ctgccgaaga ccaacctgtg
     3121 gatctacccg ggaccgcacc acgagcagaa cgtcgcgcgc ttcgccaccg accgcaacgc
     3181 ggacttcccg ctgctctatg tctccttccc gtcggccaaa gacccggagt gggaccggca
     3241 ctatcccggc aagagcacgg tgcaggtcat caccatggcg ccgtacgagt ggttcgcgca
     3301 gtggcagggc agcacctgga accagcgcgg cgcggactac gaggcgctca agcagcagtt
     3361 caccgagcgc atgctcgcgg cgctgtaccg cgagatgccg cagctgcagg gcaggctgga
     3421 catcgccgaa ctggcgacgc cgctgtcgac gcagtggttc cacctttacg accgcggcga
     3481 gatctacggc ctcgaccacg acgcgcagcg cttcagcgac gactggctgc agagcaccac
     3541 gcgcctggcc ggcctgtatc tcaccggcca ggacgtggtt accgccggcg tcggcggcgc
     3601 catgatggcc ggcgtgctca ccacggccgc gatgctcggc ctgcagtcgc gcaaggtgtg
     3661 gaagctgctt aagcagtggg cgcccgcggc ggcgcacaac ggcgcgacgg cgccggtgtc
     3721 cgcctagcgc cacacgttca gcacttctgg tatttccagt tgcgcggctt gccctcggcc
     3781 atccactcga tggcctgcag cagccggcgc tggcgcgtct cttcggtctt ggcctcggtg
     3841 atccactcga cgtagtcgcg ccggttggtg ttgctgaaag cctcgtaggt cgtcctcgcc
     3901 ttcgcgtttt tcttcagcgc tgcggcgaga tcggccggcg cctgcggcgg cggcttgcgc
     3961 gcggccttgg gcttttccac cttggtgccg ctgtcgttga gcgccatcgc gcgccggatc
     4021 agttcgagca gttgcgcgtc cggcggcaag tcatccaggc cggtgatgcg gccgaagctg
     4081 cccatggcgt cgcggttgag cgcactgccc tcggccagca gcagcttgtg cttccagaag
     4141 ccgaaggcgc agtgctgctt gaacgccgcc atgctgcaca tcattccgcg gtagtcgaag
     4201 tgcgggaagc tccacttcat tttttcctcg gcctgcggac aggcctggtg caccagcttg
     4261 cgcaggtggc ggaggatggg ctgcgcgtag ggcgcggcgt cgcggatgta ccggtcgacc
     4321 tgcggatttt tcttcatcgg cgcatcgtcg ttctggcgat gcgcgcacct taccaccgcg
     4381 ccgctacatt ttgtcgggcg gcttgtacat agccgacggc cgccagtcga cggcgccgaa
     4441 gcgcgtgctg cccagcagcg tgtaggcgat gctcaccgtg cccacgcgct ggcgctgcgc
     4501 gggattgtcc gggtactgcg cctcgcgttg cgacagcgcg tacttgagcg tgacgccgtg
     4561 cagcccccag atgcgccagg tgatcgaggt gtcggcgcgc gcgatgttct cgctgccgcc
     4621 ggtctcggtc gaggcgatgc cgctgatgta gtagtcacgc aggttgaaat cccaggccac
     4681 gcggctgccg aagatggcac gcgcc
//
LOCUS       DASZZC010000034         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_453057,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000034 DASZZC010000000
VERSION     DASZZC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453057"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1653
                     /locus_tag="VHE37_00840"
     CDS             70..1653
                     /locus_tag="VHE37_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT34099.1"
                     /translation="MQHTTEDTRSLWMDGAHLPIASPLMADTETGVCIVGAGIAGLST
                     AYLLLQHGHDVLVLDDKTIAGGQTARTSAHLSCVIDDRYTEIERVHGEAGALLAQESH
                     RAAIELIERIVQDEQIDCDFERVDAYLMAGDTRSADDLRAELAAARRAGLSDAVEAQL
                     PLPDLAQMPAIRFPRQAQFHPLKYIDGLARAVTRLGGRIHTRTHVSSVKGGAPALVRT
                     SSGLNVRAHSVVVATNTPINDRAVLHTKQAPYTTYVVGITVPRGVIPHALYWDMDDPY
                     HYVRVQRMGDDEVLIVGGEDHKTGQAHDKSQRFTRLVEWARARFPQLGELRYSWSGQV
                     YETLDGLAYIGHNPLDRNNVYVVSGDSGMGLTHGTLAALILSALIDRREHPWAALYDP
                     ARKPLKTLTTFVQENLNVARQYGEWLVPEEVKSAHDVLPGWGAVVQDGAAKHAVYRDD
                     DGTLSVCSAVCPHLGCVVAWNAVEKIWDCPCHGSRFDRYGHVISGPAVDDLAPVATEL
                     DEPPRLLPGVLPTREIPPP"
BASE COUNT          299 a          645 c          637 g          248 t
ORIGIN      
        1 cgggcgcgcc gccggtgaac tgcatgcctc cttggcgcgt cccatatcag gggccaccgg
       61 agagcttgca tgcagcacac caccgaagac acgcgttcgc tgtggatgga cggcgcgcac
      121 ctgccgatcg cctcgccgct aatggcggac accgagaccg gcgtgtgcat cgtcggcgcc
      181 ggcatcgccg gcctctccac cgcctacctg ctgctgcagc acggccacga cgtgctggtg
      241 ctggacgaca agaccatcgc cggcggccag accgcgcgca ccagcgcgca tctctcctgc
      301 gtgatcgatg accgctacac cgagatcgag cgcgtgcacg gcgaggccgg cgcgctgctg
      361 gcgcaggaga gccaccgcgc cgccatcgag ctgatcgagc gcatcgtgca ggacgagcag
      421 atcgactgcg acttcgagcg cgtcgacgcc tacctgatgg ccggtgacac gcgcagcgcc
      481 gacgatctgc gcgcggaact ggcggcggcg cggcgcgccg gcctgtccga cgcggtagaa
      541 gcgcagctgc cgctgcccga cctggcgcag atgccggcga tccgttttcc gcgccaggcg
      601 cagttccatc cgctgaaata catcgatggc ctggcgcgcg ccgtcacccg gctgggcggg
      661 cgcatccaca cgcgcacgca cgtgagctcg gtcaagggcg gcgcgccggc gctggtgcgc
      721 accagcagcg ggctcaatgt gcgcgcgcat tctgtcgtcg tcgccaccaa cacgccgatc
      781 aacgaccgcg cggtgctgca caccaagcag gcgccctaca cgacgtacgt ggtcggcatc
      841 accgtgccgc gcggcgtcat cccgcacgcg ctgtactggg acatggacga tccctaccac
      901 tacgtgcgcg tgcagcgcat gggcgacgac gaggtgctga tcgtcggcgg cgaggaccac
      961 aagaccggcc aggcgcacga caagagccag cgtttcaccc gcctggtgga atgggcgcgc
     1021 gcgcgcttcc cgcagctggg cgagctgcgc tacagctggt cgggccaggt gtatgaaacg
     1081 ctcgacggcc tggcctacat cggccacaac ccgctcgacc gcaacaacgt gtacgtggtc
     1141 agcggcgact ccggcatggg gctcacccac ggcacgctgg ccgcgctgat cctgtcggcg
     1201 ctgattgacc ggcgcgagca tccgtgggcc gcgctgtacg acccggcgcg caagccgctg
     1261 aagacgctga ccaccttcgt acaggaaaac ctcaacgtgg cgcgccagta cggcgagtgg
     1321 ctggtgccgg aggaagtgaa gtccgcgcac gacgtgctgc cgggctgggg cgcggtggtg
     1381 caggacggcg cggccaagca cgcggtgtac cgcgacgacg acggcacgct cagcgtctgc
     1441 tcggcggtgt gcccgcacct gggctgcgtg gtggcgtgga acgcggtgga gaaaatctgg
     1501 gactgcccgt gccacggttc gcgcttcgac cgctacgggc acgtgatcag cgggccggcg
     1561 gtggatgacc tggcgccggt agccacggaa ctggatgagc ctccgcggct gctgccgggc
     1621 gtgctgccca cgcgcgaaat cccgccgccg tagcgctttc cggcgacgaa ctccggcgcg
     1681 cggaccgggt cgcattcatg gaaccccgcg aggagcaggc catgaaagca gtggtattcg
     1741 aaggcatcgg cgacatccgc ctggaagacg tgcccgaacc cacgctggtc gagccgaccg
     1801 acgccatcgt gcgcctgacc gcctcggcc
//
LOCUS       DASZZC010000035         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1224112,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000035 DASZZC010000000
VERSION     DASZZC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224112"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1494
                     /locus_tag="VHE37_00845"
     CDS             22..1494
                     /locus_tag="VHE37_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVT34100.1"
                     /translation="MTTREGGPVKIFRDEDVLDTWFSSALWPFSTLGWPDRTPELAKF
                     YPTSILVTGFDIIFFWVARMVMMGQYFMHDVPFRDVYITGLVRDPEGNKMSKSKGNVL
                     DPLDVVDGIALDDLVKKRTTGLLKPETAPKIEAKTRKDYPKGISPVGVDALRFTFAAL
                     ATNGRDIRFDAGRAEGYRNFCNKIWNAARFVLMNVEGQDVGLDAGAEVVLSTADRWII
                     SRLQKLEREAAQHYADYRFDLLAQSIYQFVWNEYCDWYLELTKPALASGSDAEKRGTR
                     RTLVRVLETWLRLLHPLMPFITEEIWQKVAPLAGRQGPSLMLQPYPVSQPERIDEAAE
                     ADIEWLKSFILGVRQIRGEMDIAPGKVLPLLLADAGADDRARVARMQASIEFLAKVEA
                     PKFLAAGEAAPQSSAALVGGMKLLVPMAGLIDKDAELARLARQITKLESDIEKTRTRI
                     ANPNFGKAPANVQQQAQDQLAKLEHDLVVLKQQEMSIKAL"
     gene            complement(1706..2401)
                     /locus_tag="VHE37_00850"
     CDS             complement(1706..2401)
                     /locus_tag="VHE37_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVT34101.1"
                     /translation="MKKTAASDACVARTCMRDPIRDAIVSRILDGRFPPGTRLKELAL
                     AREFNTSQAPVREALRELETLGLVESERYRGTRVRDIDIEELHHVYELRIAIEEAAAR
                     RAVPCRKEDLQRLTDEIARMQQAIRDDDVEAYMVSAVNFHRDIVQMSGNRVFVRAWES
                     MAWDVRARVAAKRIGLIGHYLDDRRRIVSALRAGQGARAGRLLRQLIEKFVQRLLAIK
                     AEAEAAPPAQRRA"
     gene            complement(2382..>3133)
                     /locus_tag="VHE37_00855"
     CDS             complement(2382..>3133)
                     /locus_tag="VHE37_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVT34102.1"
                     /translation="AQAQLEDAKAQDAALALSRAQLEHAIAVLLGTTTQAAALQPAPL
                     DLDPPHFSASAPSQLLERRPDIAAAERRVAAANAQIGVARAAWFPSFTLGAALGFESG
                     NAASWLDAPSRFWSLGPSAALTLLDFGARRAQNELARAQYDETVADYRQSVIGAYAAV
                     EDQLAAQHWLSQQAQAQMRAAQAAERALAQARERYKGGIVTYLEVVSAQNTQLQAARA
                     ELDLRVRRLDAAVQLVKTLGGGWQADEKDSR"
BASE COUNT          549 a         1055 c         1016 g          513 t
ORIGIN      
        1 ttccaatctg caaaggcaaa attgacaaca agggaaggcg gtccggtcaa aatttttcgc
       61 gacgaggacg tgctcgacac ctggttctcc tcggcgctgt ggccgttttc caccctgggc
      121 tggcccgata ggaccccgga gcttgcgaag ttctacccca ccagcatcct ggtgacgggc
      181 ttcgacatca tcttcttctg ggtggcgcgc atggtgatga tgggccagta cttcatgcat
      241 gacgtgccgt tccgcgacgt ctacatcacc ggcctggtgc gcgaccccga aggcaacaag
      301 atgtcgaagt ccaagggcaa cgtgctcgac ccgctcgacg tggtcgacgg catcgcgctc
      361 gatgacctgg tgaagaaacg caccaccggc ctgctgaaac cggagaccgc gccgaagatc
      421 gaggccaaga cgcgcaagga ttatccgaaa ggaatttcac ccgtcggcgt cgatgcactg
      481 cgcttcacct tcgccgcgct cgccaccaac ggccgcgaca tccgcttcga cgccggccgc
      541 gccgagggct accgcaactt ctgcaacaag atctggaacg ccgcgcgctt cgtgctgatg
      601 aacgtcgaag gacaggacgt aggcctcgac gccggcgcgg aagtggtgct gtccaccgcc
      661 gaccgctgga tcatctcgcg cctgcagaag ctcgagcgcg aggctgcgca gcattacgcc
      721 gactaccgct tcgacctgct ggcgcagtcg atctaccagt tcgtctggaa cgagtactgc
      781 gactggtacc tggaactgac caagccggcg ctggcctccg gcagcgacgc cgagaagcgc
      841 ggcacgcgcc gcacgctggt gcgcgtgctg gaaacctggc tgcgcctgct gcatccgctg
      901 atgccgttca tcaccgagga aatctggcag aaggtcgccc cgctcgccgg caggcagggg
      961 ccgagcctga tgctgcagcc gtacccggtg tcgcagccgg agcgcatcga cgaagcggcc
     1021 gaggccgaca tcgagtggct gaagagtttc atcctcggcg tgcgccagat ccgcggcgag
     1081 atggacattg cgccgggcaa ggtgctgccg ctgctgctgg cagacgcggg cgccgacgac
     1141 cgcgcgcgcg tggcgcgcat gcaggcgagc atcgagttcc tggcaaaagt ggaagcgccg
     1201 aaattcctcg ctgccggcga agcggcgccg caatcatctg ccgcgctggt tggcggcatg
     1261 aagctgctgg tgccgatggc cggcctgatc gacaaggatg cggaactcgc gcgcctcgcg
     1321 cggcagatca ccaagctgga aagcgacatc gagaaaacgc gcacgcgcat agcgaatccc
     1381 aacttcggca aggcgccggc gaatgtccag cagcaggcac aagatcaact agccaaactg
     1441 gaacatgacc tggtcgtact taagcagcag gaaatgagca tcaaggcact ttgaaacatc
     1501 gtcattcccg cgcaggcggg aatccagttt tttgggaagt tgcgaaaacc gggttcccgc
     1561 ttcgccggga cgacgtcggc ccaaaaaaat gcctgaccgc actgccattc agtctgttat
     1621 taattatcta tcgttttaat aaacaaacaa taattaataa cactcgaaat cgcggtgcgg
     1681 ttggagaaaa gaactgcggg caccttcagg cgcggcgctg cgcgggcggc gccgcttcag
     1741 cctccgcctt gatcgccagc aggcgctgca cgaatttttc gatcagctgg cgcagcagcc
     1801 gtccggcgcg cgcgccctgc cccgcgcgca gcgcggaaac gatcctgcgg cggtcgtcga
     1861 ggtaatggcc aatcaggccg atgcgtttgg ccgccacgcg cgcgcgcacg tcccaggcca
     1921 tcgactccca cgcgcgcacg aacacgcggt tgccggacat ctgcacgatg tcgcgatgga
     1981 aattcaccgc gctgaccatg taggcctcga catcgtcgtc gcggatggcc tgctgcatgc
     2041 gcgcgatctc gtcggtgagc cgctgcaagt cttccttgcg gcacggcacc gcgcgccgcg
     2101 ccgcggcttc ctcgatggca atgcgcaatt cgtagacgtg gtgcagttcc tcgatgtcga
     2161 tgtcgcgtac gcgcgtgcca cggtagcgct cgctctccac caggccgagc gtttccagtt
     2221 cgcgcagcgc ctcgcgcacc ggcgcctggc tggtgttgaa ctcgcgcgcc agcgccagtt
     2281 ccttcagccg cgtgcccggc gggaagcggc cgtcgaggat gcgcgagacg atggcgtcgc
     2341 ggatcgggtc gcgcatgcag gtgcgcgcca cgcaggcatc gctagcggct gtctttttca
     2401 tccgcctgcc atcccccgcc cagtgtcttg accagttgca ccgccgcgtc gagccgccgc
     2461 acgcgcaggt cgagctcggc gcgcgccgcc tgcagctgcg tgttctgcgc gctgaccact
     2521 tccagatagg tgacgatgcc gcccttgtag cgttcgcgcg cctgcgccag cgcgcgctcc
     2581 gcagcctgcg ccgcgcgcat ctgcgcctgc gcctgctgcg acagccagtg ctgcgcggcc
     2641 agctggtctt ccaccgcggc ataggcgccg atcacgctct gccggtaatc ggccacggtc
     2701 tcgtcgtact gcgcgcgcgc cagctcgttc tgcgcgcggc gcgcgccgaa gtccagcagc
     2761 gtgagcgcgg ccgaaggacc gagtgaccag aagcggctgg gcgcatccag ccagctggcg
     2821 gcgttgccgc tctcgaaacc cagcgcggcg ccgagcgtga agctgggaaa ccaggccgcg
     2881 cgcgccacgc cgatctgcgc gttggcggcg gcaacgcggc gctcggccgc ggcgatgtcc
     2941 gggcgccgct ccagcagctg cgacggcgcg ctggcggaaa aatgtggtgg gtcaagatcc
     3001 agcggcgccg gctgcagcgc ggcggcctgc gtcgtcgtgc cgagcagcac ggcgatggcg
     3061 tgttcgagct gcgcgcgcga cagcgccagc gcggcatcct gcgctttcgc atcttccagc
     3121 tgcgcctgcg cct
//
LOCUS       DASZZC010000036         9068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_453311,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000036 DASZZC010000000
VERSION     DASZZC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453311"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VHE37_00860"
     CDS             complement(<1..952)
                     /locus_tag="VHE37_00860"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVT34103.1"
                     /translation="MNAFVPGQLLQNASAAAGGLSDFGDDSFREGLAVLCDSLSREAG
                     LSEMGVAILQQKITGQLANRLYVEDHFRRHPEIAREEVTAPLVIVGLPRTGTTKLQRL
                     LSRDPRFWFMYFWESQFPVPLPGETLKEPKARIGAAREMVSMMTQVMPKLMAIHPMDA
                     DEADEEVMLMEHSFLSAFNAYAYVPSYTRWLDRQDQAPAYAYLKRMLQFLQWQKRQRG
                     ISGQRWVLKSPHHLLRMDALLRAFPGVQVILTHRDPVQSIPSIASFIHTLWGIYATDA
                     SAQAAGAEWSALMKRALEHTMAVRRTAPGQFLDVQFRDTVK"
     gene            complement(955..2133)
                     /locus_tag="VHE37_00865"
     CDS             complement(955..2133)
                     /locus_tag="VHE37_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HVT34104.1"
                     /translation="MQEVLDTHHRHDAGGVPDGERRMTAESSIERDVISGKEWDEFCD
                     ALKRAGAQVLRPETPATALDRAEGWRYLTRLLRIGLEMHLEFADPDFPGFLAPSHETG
                     KIGADNPDNLYLMGRLNGRNDYVIRGNRGTVSYLSFGTQKGGYETNGRMEQTGFIDSA
                     NLETDAEGRFELVLSQQQRPGNWLRLEPETNAVIVRQTFLDRQAEQPAQLTIARLASD
                     ARPEPLTPARLHAGLANAAKFVEGTAKLFADWAQSYQPRVNQLPPADQALCQRAGGDP
                     NIFYYHSYWKLAPDEALVIHLARIPECRFWNLQVNNYWMESLDYRHHRIHHNKHSAKP
                     DADGGVTLVLAHQRPASSNWLETAGHSSGTLCLRWVGAKEHVHPATRVVKFSELAQGK
                     "
     gene            complement(2198..3136)
                     /locus_tag="VHE37_00870"
     CDS             complement(2198..3136)
                     /locus_tag="VHE37_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34105.1"
                     /translation="MTETQKPRVLFVDDEPRILSTMRMLFKANYELYFAESGAKALEM
                     LKTQAVDVIVSDQRMPGMTGIELLRQARELNPNAMRILLTGYSDLNAIIGSVNDGEIF
                     RFVNKPWNNEELSNTVAKAVVAAKGSAAATAKGGDAVVAGPVTGTGVLVLDEDPSVPP
                     QIQQIVGPENKVYGTTTMEQAVDVLEKEKIGVVISETRVQDNPVINLISTLKQHRPEL
                     VSVILTDHADAGAAIGLINQGQIYRYIVKPVQSTQAKIAVNSAMKQHQRLAQNPDLTQ
                     RYEVQAPPAAAAPQPGSANAGMIDRIKALRWGKKPA"
     gene            complement(3154..5040)
                     /locus_tag="VHE37_00875"
     CDS             complement(3154..5040)
                     /locus_tag="VHE37_00875"
                     /inference="COORDINATES: protein motif:HMM:NF039312.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT34106.1"
                     /translation="MNMRQLLLALATGVAIGVLLFLVQKTQAVNGDLHRERLANLRAI
                     NDLDVQLEKLLTRERVPTLEDSQQLAQKAPGLPLPDDDVSLRRVIEQLGDAQDALEKG
                     PQGLKGLNAEIDKKLARFLSTLQDKAEIAFDFKARSALGTQQLVNSVNSVPVMADAAA
                     ALLPRDAQREQMVEWLNQLKAETSDFAITASPGNTGSIIALLDQLDQAAAKKDARLKS
                     ALATLRARCNDVIAGKRDLVKQLNDFLRQDTGRQLQDLEQAYVNWHEERVNEAARYRI
                     GLVAYSVVLLGVLALVGVRLATSFRQLDRAYAKIAHANANLETQVRERTADLTMAMAD
                     LQNSQAQLVQSEKMASLGQLVAGVAHEINTPLGYARSNNDIVRDSLAGMRDLCLAQHK
                     ALMMLTAEGVSDEDVAAAMEEAQVRGQSINAEELVQDLNNLLDDSEHGLTSIADLVMT
                     LKDFSRVDRSRSDRVNLNDCSEGALKISHNALKHHVEVVRDYGELPLVECAPSQINQV
                     FLNLYTNAAQAMSQPGKLFITTRAEGGNAVIRVRDTGSGMTDEVKKKIFEPFFTTKPV
                     GVGTGLGLSIVFRIIEDHHGKVEVNSTVGVGTEFVITLPLKQPRKTDKIATQEEVTTA
                     LAAA"
     gene            5241..5741
                     /locus_tag="VHE37_00880"
     CDS             5241..5741
                     /locus_tag="VHE37_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT34107.1"
                     /translation="MDVAALERRLQKLEDAEAIRELKSRYFAACDAKDAAVMRACFAD
                     GKVHIDYGVVGTFDTADALVQVFKQVGCHDYMVEMHHGANPQIEVLDAQRARGSWSLH
                     YFLINTRDHTLTQLGGMYADEYRKTASGWKISSTVFTATSTLVFNIADGSAKALFAGR
                     SAPMPG"
     gene            5909..6175
                     /locus_tag="VHE37_00885"
     CDS             5909..6175
                     /locus_tag="VHE37_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34108.1"
                     /translation="MRSMIAALLSLVVVSSSLEVQDAVYPCTDGQGNTYYSAGGCKLG
                     DKPAVQINATGTPMVTHVPQAPQVPMAPQVQTPPPASRPAVPLK"
     gene            6186..6467
                     /locus_tag="VHE37_00890"
     CDS             6186..6467
                     /locus_tag="VHE37_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34109.1"
                     /translation="MRASILFGLLTLLVVQAAAARGLYECAGPKGEHYYSDKGCKNPD
                     PPPPEPGGTLTLLPGKNQPASPTIKNGFATQNRYTQRAEAGAAKAQQHQ"
     gene            6515..7309
                     /locus_tag="VHE37_00895"
     CDS             6515..7309
                     /locus_tag="VHE37_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HVT34110.1"
                     /translation="MIAFPHTPLQWYQDLLWLLLLSCPLVFGGLMFLPAAYGRHKQGH
                     SKWWGPGIPTRWAWVIMECPSSLGFAAFYFMGDRAWQPAPLVLFLMWQSHYFQRSFIF
                     PFRIKPRPGDTTPVSIPLMAVSSNLVVSFLNAAMLSWSSVGADYASNWLLDPRFLLGL
                     AIFIVGYRTNRKADAMLAALRKPGETGYKIPRGWLYEKISCPNYFGEYLIWTGWAIAT
                     WSVAGLAFVLWTLANLVPRALQNHRWYHQKFTDYPRERRAVIPYVL"
     gene            7331..7960
                     /locus_tag="VHE37_00900"
     CDS             7331..7960
                     /locus_tag="VHE37_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34111.1"
                     /translation="MNHNKKMICAALVALVWSASSSATTKGLSQIVTPDLQDPGALSV
                     SAQLQSRAIANPYQLQAELGLTRWFEVAMFQGLSPGEEIFGSEIGLYNQGPWLLTAGF
                     INWSTRGVRSQPFLEGGYYTESDKFMGGPIQAHGKTELLLGWAHDFDPRWRFQLDYQS
                     GGDNYVTAGFTCNVTPAFQFNPAVYVSNNDASHAVGYIVFTYTLPVWKP"
     gene            complement(7973..9010)
                     /gene="dmpG"
                     /locus_tag="VHE37_00905"
     CDS             complement(7973..9010)
                     /gene="dmpG"
                     /locus_tag="VHE37_00905"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HVT34112.1"
                     /translation="MNLKGVKIKVPDKSLRDGMHPKRHQITIEQMKSIAKGLDESGVP
                     LIEVTHGDGLGGASVNYGFPAATDEEYLRAVIPLLKKAKVSALLLPGIGTVDHLRMAK
                     DCGVTTIRVATHCTEADVSEQHIALGRKLGMDTVGFLMMAHMIEPDAILKQALLMESY
                     GANCLYVTDSAGYMLPDDVSARIGLLREKLKPETEIGFHGHHNLSMGVANSLAAVEAG
                     ARRIDCACGGMGAGAGNTPMEVFVAVCNRMGIETGVDVFKVSDVAEDLVTPIMDFPVR
                     IDRNSLTLGYAGVYSSFLLFARRAEAKYGVSAREILLELGRRKMVGGQEDMIEDTAIT
                     MARERGLKTVA"
BASE COUNT         1402 a         2951 c         3000 g         1715 t
ORIGIN      
        1 gcttcaccgt gtcgcggaac tgtacgtcga gaaattgccc tggcgcagtg cggcgcacgg
       61 ccatggtgtg ctccagcgcg cgcttcatca gcgcgctcca ctccgcgccc gccgcctgcg
      121 cgctcgcgtc ggttgcgtag atgccccaca gcgtgtggat gaagctggca atggacggga
      181 tgctctgcac cgggtcgcgg tgggtcagga tcacctgcac gccggggaag gcgcgcagca
      241 gcgcgtccat gcgcagcagg tgatgcggcg acttcagcac ccagcgctgg ccggaaatgc
      301 cgcgctggcg cttctgccac tgcaggaact gcagcatgcg cttgagatag gcgtaggccg
      361 gtgcctggtc ctgccggtcg agccagcggg tgtaagaagg aacatatgcg taggcgttga
      421 acgccgacag gaaggaatgc tccatcagca tcacttcctc atcggcctcg tcggcgtcca
      481 tcgggtggat ggccatgagt ttgggcatga cctgcgtcat catcgacacc atctcgcgcg
      541 cggcgccgat gcgcgccttc ggctccttca gcgtctcgcc gggcagcggc accgggaact
      601 gcgattccca gaaatacatg aaccagaagc gtggatcgcg cgacagcagg cgctgcagct
      661 tggtggtgcc ggtgcgcggc aggccgacga tgaccagcgg ggcggtgact tcctcgcgcg
      721 cgatctccgg gtggcgcctg aagtggtcct cgacgtaaag acggttggcc agctggccgg
      781 tgatcttctg ctgcaggatg gccacgccca tctccgacag gccggcctcg cggctgagcg
      841 aatcgcacag caccgccagg ccttcgcgga agctgtcatc gccaaaatcg ctgagtccgc
      901 cggcggcggc gctggcgttc tgcagcagct gcccgggcac gaatgcattc atgttcattt
      961 tccctgcgcg agttcggaaa acttcactac gcgcgtggcc ggatgcacat gctctttggc
     1021 gccgacccag cgcaggcaca gcgttccact cgagtgcccg gccgtctcca gccaattgga
     1081 cgaggccggc cgctggtgcg cgagcaccag cgtaacgccg ccgtcggcgt cgggcttcgc
     1141 gctgtgcttg ttgtgatgga tgcggtgatg gcgatagtcg agcgactcca tccagtagtt
     1201 gttcacctgc aggttccaga agcggcattc ggggatgcgc gccaggtgaa tgaccagcgc
     1261 ctcgtccggc gccagcttcc agtacgagtg gtagtagaag atgttcgggt cgccgcccgc
     1321 gcgctggcac agcgcctggt ccgccggcgg cagctggttc acgcgcggct ggtagctctg
     1381 cgcccagtcg gcgaagagtt tcgcggtgcc ttcgacgaat ttcgccgcgt tggccagccc
     1441 cgcgtgcagg cgcgccggcg tgagcggctc gggcctggca tcgctggcca ggcgggcgat
     1501 ggtgagctgt gccggctgct cggcctggcg gtcgaggaag gtctggcgca cgatcaccgc
     1561 gttggtctcc ggctccaggc gcagccagtt gcccggccgc tgctgctgcg acagcaccag
     1621 ctcgaaacgg ccctcggcgt cggtctcgag gttggcggaa tcgatgaagc cggtctgctc
     1681 catccggccg ttggtctcat acccgccctt ctgcgtgccg aaggagagat acgaaacggt
     1741 gccgcggttg ccgcggatca cgtagtcgtt gcggccgttg agccggccca tcaggtagag
     1801 attgtccggg ttgtcggcgc cgatcttgcc ggtctcgtgc gagggcgcga ggaagccggg
     1861 aaagtccggg tcggcgaatt ccaggtgcat ctccaggccg atgcgcagca gccgcgtgag
     1921 ataacgccag ccctcggcgc ggtccagcgc ggtggccggc gtttccgggc gcagtacctg
     1981 cgcgccggcg cgcttgagcg catcgcagaa ttcgtcccac tccttgccgc tgatcacgtc
     2041 ccgctcgatg ctgctctcag ccgtcatgcg tcgttctcca tcgggcaccc cgccggcatc
     2101 gtgccggtgg tgcgtgtcga gcacctcctg cattcgcagc agtcgcgcca aaaaaaacgc
     2161 cgcccgggtg ggcggcgcgt ggtgatgcgg aaaaacttca ggccggcttc ttgccccagc
     2221 gtagggcctt gatgcggtcg atcatgccgg cattggcgct gcccggctgc ggcgcggcag
     2281 cggctggcgg tgcctgcacc tcgtagcgct gcgtcaggtc cgggttctgc gccaggcgct
     2341 ggtgctgctt catcgccgag ttgaccgcga tcttggcctg cgtgctctgc accggcttga
     2401 cgatgtagcg gtagatctgg ccctggttga tcaggccgat ggcagcgcca gcatcggcgt
     2461 ggtcggtgag gatcaccgac accagttccg ggcggtgctg cttgagcgtg gagatcaggt
     2521 tgatcaccgg gttgtcctgc acgcgcgttt cggagatgac cacgccgatc ttctccttct
     2581 ccagcacgtc caccgcctgc tccatggtgg tggtgccgta gaccttgttc tccgggccga
     2641 cgatctgctg gatctgcggc ggcaccgagg ggtcttcatc cagcaccagc acgccggtgc
     2701 cggtgaccgg gccggcgacg acggcatcac cgcccttggc ggtggcggcg gcgctgccct
     2761 tggcggcgac cacggccttg gccacggtgt tggacagttc ctcgttgttc cacggcttgt
     2821 tgacgaagcg gaagatctcg ccgtcgttga ccgagccgat gatggcgttg aggtcggagt
     2881 agccggtgag caggatgcgc atggcgttgg gattgagctc gcgcgcctgg cgcagcagct
     2941 cgatgccggt catgccgggc atgcgctggt ccgacacgat gacgtcgacc gcctgggtct
     3001 tgagcatctc cagcgccttg gcgccgctct cggcgaaata cagctcgtag ttcgccttga
     3061 acagcatgcg catggtggaa aggatgcgcg gttcgtcgtc gacgaacagt acgcggggct
     3121 tttgcgtctc ggtcatgaca gcgactctcc tgatcaggcc gcggcgagcg cggtcgtaac
     3181 ttcttcctgg gtggcgatct tatcggtttt gcgcggctgt ttgagtggca gggtaatgac
     3241 gaactccgtg cccaccccca cggtggagtt tacttcgact ttgccatggt ggtcttcgat
     3301 gatgcggaac acgatggaca gccccagccc ggtgcccacg cccaccggct tggtggtgaa
     3361 gaacggctcg aaaatcttct tcttcacttc gtcggtcatg ccggagccgg tgtcgcgcac
     3421 gcggatcacc gcgttgccgc cctcggcccg cgtggtgatg aacagcttgc ccggctggct
     3481 catggcctgc gccgcgttgg tgtacaggtt gaggaacacc tggttgatct ggctgggcgc
     3541 gcactccacc agcggcagtt cgccgtagtc gcgcaccacc tcgacgtggt gcttgagcgc
     3601 gttgtggctg atcttcagcg cgccctcgct gcagtcgttg aggttgacgc ggtcgctgcg
     3661 cgagcggtcg acgcgcgaga aatccttcag cgtcatcacc aggtcggcga tggaggtgag
     3721 gccgtgctcg gaatcgtcga gcaggttgtt gaggtcctgc accagctcct cggcgttgat
     3781 cgactggccg cgcacctgcg cttcttccat ggccgcggcc acgtcctcgt cggacacgcc
     3841 ttcggcagtg agcatcatca gcgccttgtg ctgggccagg cacaggtcgc gcatgccggc
     3901 cagcgaatcg cgcacgatgt cgttgttgct gcgcgcgtag cccagcggcg tgttgatctc
     3961 gtgcgccacg ccggcgacca gctggccgag cgaggccatc ttttccgact gcaccagctg
     4021 cgcctgcgag ttctgcagat cggccatggc catggtcagg tcggcggtgc gctcgcgcac
     4081 ctgtgtttcc aggtttgcgt tggcgtgcgc gatcttggcg taggcgcggt ccagctggcg
     4141 gaagctggtc gccaggcgca cgccgaccag cgccagcaca ccgagcagga ccacggagta
     4201 ggcgaccaga ccgatgcggt agcgcgcggc ctcgttgacc cgctcctcgt gccagttgac
     4261 gtaagcctgc tcgaggtcct gcagctggcg cccggtgtcc tgcctgagga aatcgttgag
     4321 ctgcttgacc aggtcgcgct tgccggcgat gacatcgttg cagcgcgccc gcagcgtggc
     4381 cagcgcgctt ttcagccgcg cgtccttctt cgctgcggcc tggtccagct ggtcgagcag
     4441 ggcgatgatc gagccggtgt tgcccggcga tgcggtgatg gcgaagtccg aagtctccgc
     4501 cttcagctgg ttgagccact ccaccatctg ctcgcgctgg gcgtcgcgcg gcagcagcgc
     4561 ggcggcggca tcggccatca ccggcaccga attgacgctg ttgaccagct gctgcgtgcc
     4621 cagcgcgctg cgcgccttga agtcgaaggc gatctcggcc ttgtcctgca gcgtgctgag
     4681 gaagcgcgcc agtttcttgt cgatctccgc gttcaggccc ttgagccctt gcggaccttt
     4741 ttccagcgcg tcctgggcgt cgcccagctg ttcgatcacc cggcgcagcg acacgtcgtc
     4801 gtcgggcagc ggcaggcccg gtgctttctg ggcgagctgc tggctgtctt ccagcgtggg
     4861 cacgcgctcg cgcgtgagca gcttttccag ctgcacgtcc aggtcgttga tggcgcgcag
     4921 gttggccagc cgttcgcggt gcaggtcgcc gttgaccgcc tgcgtcttct gcaccaggaa
     4981 cagcagcacg ccaatggcga cgcccgtggc cagggccagc aacaattggc gcatgttcat
     5041 tcctctctcc cctcacactg cgatgccggg gcccgtccgt agcgggcctg attcgcgcaa
     5101 gcgcggctac cttaacgacg gcgtccgcct gcgccttcac ctgtttgcag tagcttagtc
     5161 cgcggctttc ctgatgcaaa tgggtgatac gcactaggtc gctttgccgt gtagtcgcag
     5221 cccatcaacc ggagaccgtc atggatgttg ccgccctcga acgacgcctg cagaaactcg
     5281 aagacgccga ggccatccgc gagctgaagt cgcgctactt cgccgcctgc gacgccaagg
     5341 acgcggccgt gatgcgggcc tgttttgccg acggcaaggt gcatatcgat tacggtgtgg
     5401 tcggcacttt cgacaccgcc gatgcgctgg tgcaggtgtt caagcaggtc ggctgccacg
     5461 actacatggt ggagatgcac cacggcgcca atccgcagat cgaggtgctg gatgcgcagc
     5521 gcgcgcgcgg cagctggagc ctgcattact tcctgatcaa cacgcgcgac cacacgctga
     5581 cccagctggg cggcatgtat gcggatgagt accgcaagac cgcgtcgggc tggaagatca
     5641 gcagcacggt gttcaccgcg acttccacgc tggtgttcaa catcgccgac ggatctgcca
     5701 aggccctgtt tgccggccgc agcgcaccga tgcccggctg atgccgcgtg gagccctcca
     5761 ccgcatcccg gttttgcgtg ttattaaaga tattatataa gtaaaatagt taaatatatt
     5821 gtaacatcat ttccgagggt gcggtaaggg attgctgacg tgcgatcggg cctccgcagc
     5881 tgccgtcctc gcgctggatt ggagtagcat gcggagcatg atcgcggcgc tgctctcgct
     5941 ggttgtcgtc tcgtcctcgc tcgaggtgca ggacgccgta tatccgtgca ccgacgggca
     6001 gggcaacacc tattacagcg caggcggctg caagcttggc gacaagccgg cagtgcagat
     6061 caacgccacc ggcacgccga tggtgacgca cgtcccgcag gcgccgcagg tgccgatggc
     6121 gccgcaggtg cagacgccgc cgcccgccag ccggccggcc gtgccgctca agtgacgcgg
     6181 cgcccatgcg cgcatcgatc ctcttcgggc tgctgaccct gctggtggtg caggctgccg
     6241 cggcacgcgg cctgtacgaa tgcgccggtc ccaagggcga gcattactac agcgacaagg
     6301 gctgcaagaa tcccgatccg ccaccgcccg agcccggcgg cacgctgacc ctgctgccgg
     6361 gcaagaacca gccggcatcg cccaccatca agaacgggtt cgccacgcag aaccgttaca
     6421 cgcagcgcgc cgaagccggc gccgccaagg cgcagcagca ccagtagccc ggcgccatcg
     6481 cggtgccgtc atccgctgcg tttaagatgc gcgcatgatc gcgttcccgc acaccccgct
     6541 gcagtggtac caggacctgc tgtggctgct gctgctgtcg tgcccgctgg tgttcggcgg
     6601 cctgatgttc ctgcccgcgg cctatggccg ccacaagcag ggccacagca agtggtgggg
     6661 gccgggcatt cctacgcgct gggcctgggt gatcatggaa tgcccgtcgt cgctgggatt
     6721 tgcagcgttc tacttcatgg gcgaccgtgc ctggcagccg gcgccgctgg tgctgttcct
     6781 gatgtggcag agccactatt tccagcgcag tttcatcttc ccgttccgca tcaagccgcg
     6841 gccgggcgac accacgcccg tctcgatccc gctgatggcg gtgagttcca acctggtggt
     6901 gtcgttcctc aacgccgcca tgctgtcgtg gtcgtcggtc ggcgccgact acgcgtcgaa
     6961 ctggctgctc gatccgcgct ttctgcttgg gctggccatc ttcatcgtcg gctaccgcac
     7021 caaccgcaag gccgacgcca tgctcgccgc gctgcgcaag ccgggcgaaa cgggatacaa
     7081 gattccgcgc ggctggctct acgaaaaaat cagctgcccg aattatttcg gcgaatacct
     7141 gatctggacc ggctgggcga tcgccacctg gtctgtggcg gggctggcct tcgtgctgtg
     7201 gacgctggcc aacctggtgc cgcgcgcgct gcagaatcac cgctggtacc accagaaatt
     7261 tacggattat ccgcgcgagc ggcgggcggt gatcccatac gtgctgtgaa aatcgcgcag
     7321 ggagagcatc gtgaatcaca acaagaagat gatttgcgcg gcgctggtcg cgctggtctg
     7381 gtcggcatcg agcagcgcta ccaccaaggg actgagccag atcgtcacgc ccgacctgca
     7441 ggaccccggc gcgctgtcgg tcagcgcgca gctgcagagc cgcgcgatcg ccaatccgta
     7501 ccagttgcag gcggagcttg gcctcacccg ctggttcgag gtggcgatgt tccagggcct
     7561 gtcgccgggc gaggaaatct tcggctcgga gatcggcctg tacaaccagg ggccatggct
     7621 gctcaccgcc ggcttcatca actggtccac gcgcggcgtg cggtcgcagc catttctcga
     7681 aggcggctac tacaccgagt cggacaagtt catgggcggg ccgatccagg cgcacggcaa
     7741 gaccgagctg ctgctcggct gggcacacga cttcgaccca cgctggcgct tccagctcga
     7801 ttatcagagc gggggcgaca actacgtgac cgcgggcttc acctgcaatg tcacgccggc
     7861 cttccagttc aacccggcgg tgtacgtatc gaacaacgat gccagccacg ccgtgggcta
     7921 tatcgtcttc acctacacgc tgccggtgtg gaagccctga cgccggctgg cgtcaggcaa
     7981 cggtcttcag tccgcgctcg cgcgccatgg tgatggcggt gtcctcgatc atgtcttcct
     8041 ggccgccaac catcttgcgc cggccgagtt cgagcaggat ctcgcgcgcc gacacgccgt
     8101 acttggcttc ggcgcggcgt gcgaacaaca ggaagctcga atacacaccg gcgtagccga
     8161 gcgtgagcga gttgcggtcg atgcgcaccg ggaaatccat gatcggcgtg accaggtcct
     8221 cggccacgtc gcttaccttg aacacgtcta cgccggtttc gatgcccatg cggttgcaca
     8281 ccgcgacgaa cacttccatc ggcgtgttgc ccgcgcccgc gcccatgccg ccgcaggcgc
     8341 agtcgatgcg ccgcgcgcca gcctcgaccg cggccagcga gttggccacg cccatcgaca
     8401 ggttgtggtg gccgtggaaa ccgatctcgg tttcgggttt cagcttttcc cgcagcaagc
     8461 cgatgcgggc tgagacgtcg tcgggcagca tgtagccggc gctgtcggtg acgtagagac
     8521 aattggcgcc gtagctttcc atcagcagcg cctgcttgag gatcgcgtcc ggctcgatca
     8581 tgtgcgccat catcaggaaa cccacggtgt ccatgccgag cttgcggccc agcgcgatgt
     8641 gctgctcgga cacgtcggcc tcggtgcagt gcgtggccac gcggatggtg gtgacgccgc
     8701 agtccttcgc catgcgcagg tgatcgaccg tgccgatgcc cggcagcagc agcgcggaca
     8761 ccttggcttt cttgagcaga ggaatcaccg cacgcaggta ttcctcgtcg gtcgcggcgg
     8821 gaaagccgta gttgaccgag gcgccgccca aaccgtcgcc atgggtgact tctatgagcg
     8881 gcacaccgga ctcgtcgagc cctttggcga tgctcttcat ctgctcgatg gtgatctggt
     8941 gccgcttggg gtgcatgccg tcgcgcagcg acttgtcggg caccttgatc ttcacgcctt
     9001 tcaaattcat cgcttcactc cagactgctt tttgtaggag cgcgccttgc gcgcgattcc
     9061 cacgtcca
//
LOCUS       DASZZC010000037         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1224253,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000037 DASZZC010000000
VERSION     DASZZC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224253"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VHE37_00910"
     CDS             <1..1061
                     /locus_tag="VHE37_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase domain-containing
                     protein"
                     /protein_id="HVT34113.1"
                     /translation="SMDVLSGRGIQQGPRNALATAYKATPVAITVEGANILTRNLMIF
                     GQGAIRCHEYVFPEMEAARANDLAAFDKLLFGHIGFSINRAIRAGTHGLTGSLTAGTP
                     RSGPMAVYFKNLERMSSALAFVSDLTMFVLGGKLKFKERLSARLGDVLSQLYIASAVI
                     KYYLHHGEQADDLPHAKWALDTCLFEMARAFDDFLRNFPVGWARVLMRVVVFPLGNHY
                     APVSDALNAKIADMMLEPTALRDRITWLVHKSGGANDPIGRIEHAYDMLLEAEPAYLK
                     YYKLYSKDELAGDSIGERLEDAVRRSLLAREEAELVAEYDRVRFDVIQTDHFTKDYIA
                     GHFEAERNKVVDIATRRA"
     gene            1075..2859
                     /locus_tag="VHE37_00915"
     CDS             1075..2859
                     /locus_tag="VHE37_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34114.1"
                     /translation="MTSMQEAKAALPANWRLLLLAPGALALALYARTLAYGYVWDDTL
                     YLTYLPLAWDWDELSRAAFGVFNVFYYRPLALLLLGTERAFSAGQPMIFHLDNVLLHA
                     INSVLVAALGARVARRAGASALAAGVLSGLFYAAHPVLIEGVAWISARFDLLCTTFCL
                     LTLLLDRALLGRAMRVPAVALSFFLALCSKEMAVSLLLILPLWQWSLAPAARFTREDR
                     RVLAAAALAFVLYLLLRSHALGGMVTSPLDTWRERPLAHLDLVCRCLSGYVMLALNLF
                     AAPNPVHPMPAAAPFSTWPSWIGLGMGLVAAMLAWFAIRRRPLALLAVCFFLSLLPVL
                     QIVAVIVADNFVQERFMVFPLAFAALLAGNGWVALWAAQGFVLARRVLAGASAVFWLS
                     ASLLMTSATVPTWSDPLALWMWSYQHAPDSIVARANLLFELNGGGLRDQALSLAQYTE
                     RTQAASMTTEEYLIYAELLEESGQLEDAARKLDRAAAIAPPEEAYLPASIHAQRARLA
                     LDRNDLPTAESEFRSALRYRPRHPYSACMLGATLIALGRREEGERWFAQGLSYAPKED
                     AAQFEVSRDLLLKKATSAGVQHAPAHPR"
     gene            2990..>4230
                     /locus_tag="VHE37_00920"
     CDS             2990..>4230
                     /locus_tag="VHE37_00920"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVT34115.1"
                     /translation="MLPFRVSLRAAARIAVGCLLAASVPARAGWDNTATTALVPPAGT
                     VLGALPADTPMQVTVTLKLRNKGMLDQLVRNQHTPGNPMYGRTLTPAQVHAQFDPTSA
                     QAKMVADYLKSAGFTTVRVSKGHTLVGGTATAAVVQRAFNTQLAQATIDGVSGYLNLQ
                     PAQVPGPLAGVVLSVNGLQNVVRMKTDLQQSAARAGVTVPYRAGVLPRIEPNSASGGV
                     PDPSNSAFGPNEFQKAYDALGVTPGSNTVIAISTYGDDLKQVIADLRQAEQNNHLPYV
                     PVELRVESPIPDADMDTGGDGEWALDTQSSTGIAGNVKKLVLYTADENVPASLLWQYA
                     NFASDDSSDAPTIGNMSYGTCDVLWPVTTAAIFTYMPDMASADQEFEKAAAEGRSWHA
                     SSGDSGGACSPGNFQALPDTGP"
BASE COUNT          662 a         1392 c         1423 g          753 t
ORIGIN      
        1 acagcatgga cgtgctgtcc ggccgcggca tccagcaggg cccgcgcaat gcgctcgcca
       61 ccgcatacaa ggccacgccg gtggcgatca cggtggaagg cgccaacatc ctcacgcgca
      121 acctgatgat cttcggccag ggcgccatcc gctgtcacga atacgtgttc ccggaaatgg
      181 aagcggcgcg tgcgaatgat ctggcggcat tcgacaagct gctgttcggg cacatcggct
      241 tttccatcaa ccgcgcgatc cgtgccggca cgcatggact gaccggctcg ctcaccgcgg
      301 gcacgccgag gtcggggccg atggcggtct acttcaagaa cctggagcgc atgtccagcg
      361 cgctggcctt cgtctccgac ctgaccatgt tcgtgctcgg cggcaagctc aagttcaaag
      421 aacggctgtc ggcgcggctg ggtgatgtgc tgtcgcagct gtacatcgcc tccgcggtca
      481 tcaagtacta cctgcatcac ggcgagcagg cggacgacct gccgcacgcg aaatgggcgc
      541 tcgacacctg cctgttcgag atggcgcgcg cgttcgacga tttcctgcgc aactttccgg
      601 tgggttgggc gcgcgtgctg atgcgcgtcg tggtgtttcc gctgggcaat cactatgcgc
      661 cggtgagcga cgcgctcaac gcgaagatcg ccgacatgat gctggaaccc accgcgctgc
      721 gtgaccgcat cacctggctg gtgcacaaat ccggtggcgc caatgatccg atcgggcgca
      781 tcgagcatgc ctacgacatg ctgctcgagg ccgagccggc ctatctcaag tactacaagc
      841 tttactcgaa ggatgaactc gccggcgaca gcatcggcga gcgtctggaa gatgcggtgc
      901 ggcgcagcct gcttgcgcgc gaggaggccg agctcgtcgc cgagtacgac cgagtacgtt
      961 tcgacgtgat ccagaccgat catttcacca aggactacat cgccggccac ttcgaggccg
     1021 aacgcaacaa ggtggtggat atcgccacgc ggcgcgcatg agccacgcgc gccagtgacc
     1081 tccatgcagg aagcgaaggc cgcattgccg gccaactggc gattgttgct gctggcgccg
     1141 ggcgcgctgg cgctggcctt gtacgcgcgc acgctggctt acggttatgt gtgggatgac
     1201 acgctgtatc tcacctacct gccgctggct tgggattggg acgagctgag ccgcgccgcg
     1261 ttcggcgtgt tcaacgtctt ctattaccgg ccgctggcgc tgctgctgct tggcaccgag
     1321 cgggcgtttt ccgccgggca gccgatgatc tttcacctgg acaacgtgct gctgcacgcg
     1381 atcaacagcg tgctggtggc tgcgctgggt gcgcgggtgg cacggcgcgc gggggcctcg
     1441 gcactggccg cgggcgtgct gagcggattg ttctacgcag cgcatccggt gttgatcgaa
     1501 ggcgtggcct ggatctcggc gcgcttcgac ctgttgtgca cgacgttctg cctgctcacg
     1561 ctgctgctgg atcgcgcgct gctggggcgc gccatgcgcg tgccggccgt ggcgctcagt
     1621 tttttcctgg cgttgtgctc gaaggaaatg gcggtgagcc tgctgctgat cctgccgctg
     1681 tggcaatggt cgctcgcgcc cgcagcgcgg ttcacgcgcg aggatcgtcg cgtgctggcg
     1741 gccgccgcac tggcgttcgt gctgtacctg ctcctgcgta gccacgcgct gggcggcatg
     1801 gtgaccagcc cgctcgacac ctggcgcgag cggccgctgg cgcatctcga cctggtgtgc
     1861 cgctgcctca gcggctacgt gatgctggcg ctcaacctgt ttgccgcgcc caacccggtg
     1921 cacccgatgc cggcagcggc gccgttttcg acgtggccgt cgtggatcgg attgggcatg
     1981 gggctggtgg cggcgatgtt ggcgtggttc gccatccggc ggcgcccgct ggcattgctt
     2041 gccgtgtgtt tcttcctgag cctgctgccg gtgctgcaga ttgtcgccgt gatcgtcgcc
     2101 gacaatttcg tgcaggaacg cttcatggtt tttccgctcg cgttcgcggc attgctggcg
     2161 ggcaatggct gggttgccct gtgggctgca cagggattcg tgcttgcacg ccgtgtgctg
     2221 gcaggcgcgt cggccgtctt ctggctgtcg gcttctctgc tgatgaccag tgccacggtg
     2281 ccgacatgga gcgacccgct ggcgctatgg atgtggagtt accagcatgc gcccgattcc
     2341 atcgtcgcgc gtgccaacct gctgttcgaa ctcaatggcg gcggcctgcg cgaccaggcg
     2401 ctgagcctgg cgcaatacac cgagcgcacg caggcggcga gcatgacgac ggaggaatac
     2461 ctgatctacg ccgaactgct tgaagaatcc gggcagttgg aagatgccgc gcgcaagctc
     2521 gaccgggcgg cagccatcgc tccgccagag gaagcgtacc tgccggcgtc catccatgcg
     2581 cagcgcgcgc ggctggcgct cgaccgcaac gatctgccca cggcggagag cgagttccgc
     2641 tcggcgctgc ggtatcgacc acgccatcct tattccgcct gcatgctcgg tgcgacgctg
     2701 atcgcgctgg gccggcgcga ggagggcgag cgctggtttg cgcagggcct gtcctatgcg
     2761 ccgaaggaag atgcggcgca gttcgaagtg agccgcgacc tgctgctcaa gaaggcgact
     2821 tcggccggtg tgcagcacgc gccggcgcat ccgcgctgag tgtttcccta gagcagaaat
     2881 gaggaaacat ccctatcgac gcgcgctctc ccgcgtccga tagtcgcagc cataaatcgt
     2941 cttgcgcgcc gcgcctccgc gcggcagcag caagacccgg agaaattcca tgttgccgtt
     3001 ccgcgtttcg ctgcgcgctg ccgcgcgcat tgctgttggc tgtctgcttg ctgcatcagt
     3061 gccggcgcga gccggctggg acaacaccgc gaccaccgcg ctggtcccgc cggccggtac
     3121 cgtgctcggc gcgctgcccg ccgacacgcc gatgcaggtc accgtcactc tcaagctgcg
     3181 caacaagggc atgctcgacc agctggtgcg caaccagcac acgccgggca acccgatgta
     3241 cggccgcacg ctgacgccgg cgcaggtgca tgcgcagttc gatccgacgt cggcgcaggc
     3301 caagatggtt gccgattacc tgaaatccgc cggcttcacc acggtgcgcg tgagcaaggg
     3361 ccacacgctg gtgggcggca ccgcgacggc ggccgtggtg cagcgcgcgt tcaacacgca
     3421 gctggcgcag gcgaccatcg acggcgtcag cggttatctc aacctgcagc ctgcgcaggt
     3481 gccgggcccg ctcgccggcg tggtgctgtc ggtcaacggc ttgcagaacg tggtgcgcat
     3541 gaagaccgac ctgcagcagt cggcggcgcg cgcgggcgtg acggtgccgt atcgcgccgg
     3601 cgtgctgccg cgcatcgagc cgaattcggc atcgggcggt gttcccgatc cgtccaacag
     3661 cgcgttcggt ccgaatgaat tccagaaggc ctacgacgcg ctgggcgtga cgccgggctc
     3721 caacacggtc atcgccatct ccacctacgg cgacgacctc aagcaggtca tcgccgacct
     3781 gcgccaggcg gaacagaaca atcacctgcc gtacgtgccg gtggaactgc gcgtggagag
     3841 cccgatcccc gatgccgaca tggacaccgg cggcgacggc gagtgggcgc tggacacgca
     3901 gtcgtcgacc ggcatcgccg gcaacgtgaa gaagctggtg ctgtacaccg ccgacgaaaa
     3961 tgtgccggcc agcctgctgt ggcagtacgc caatttcgcc tcggatgatt cctccgatgc
     4021 gccgaccatc ggcaacatgt cctacggcac ctgcgatgtg ctgtggccgg tgaccaccgc
     4081 ggccatcttc acctacatgc cggacatggc ttcggccgac caggaattcg agaaggccgc
     4141 ggcggaaggc cgcagctggc atgcatcgag cggcgattcc ggcggcgcct gctcgcccgg
     4201 aaacttccag gccttgcccg acaccggccc
//
LOCUS       DASZZC010000038         6564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_644210,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000038 DASZZC010000000
VERSION     DASZZC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644210"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VHE37_00925"
     CDS             complement(<1..1229)
                     /locus_tag="VHE37_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34116.1"
                     /translation="MGIISAGSAAGGRIAEHPALTASIALRAAMVSLLWLLLLVLLMH
                     PTIVAYHPVSDEWALLQHSTASGGEVAGWFRDGFSRYFTSWPGVDTPPMQFLRPVFNA
                     VYLLLGALLGVGSGQYLYVTAAAIAACAGLVYAAGAQRQPATALPLALLLPLLPGFAG
                     SIALLFVPCFMFDALAAAACLGAYLAYQRQRYIWAMVWLALAVLAKETAVPVAAAFVV
                     HAALAWLAAPSPQRLRPVLLLAAPLAAWALLRLAAFHGIVGGVYVSGHNAGVLARMLS
                     HVDQYPYFVDYMAPGSHGKSVRLLGWLNLLLASIGVTLMAARAWRHRKLELAELALLF
                     SYVFLLVLGTSARFGASLDAFLLLSWLHWNREPPSSIWRSLATVGGALLLVGAGLYGW
                     QSAHMYDLYRDDYARMYR"
     gene            complement(1390..2196)
                     /locus_tag="VHE37_00930"
     CDS             complement(1390..2196)
                     /locus_tag="VHE37_00930"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT34117.1"
                     /translation="MAQTVFITGASTGIGAAAADMFFERGWNVAATMRTPKGERDDPR
                     WLLAKLDVTDAASIAAARDAAIAKFGRIDVLVNNAGYGLVGTFESMDDERIKKQFETN
                     VFGLMRTTRALLPHFRANRGGTVVNVASMGGRLTFPFYSVYHATKWAVDGFSESLAFE
                     LEHVGVRVKIIEPGAIKTDFYERSMDFVHDRSLAEYNDIVDKGMRRMAKAGGRGAPAR
                     LVAEAIWKAANDRSGTLRYVVGRDAKSLMVMRRFMQGAHYRKMISSQLLR"
     gene            2263..4521
                     /gene="uvrD"
                     /locus_tag="VHE37_00935"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             2263..4521
                     /gene="uvrD"
                     /locus_tag="VHE37_00935"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HVT34118.1"
                     /translation="MQFPVPDLDPNLVPLVENLNPAQKEAVTAPPEHRLVLAGAGSGK
                     TRVLTHRLAWLVAAEGVSPHSILAVTFTNKAAAEMRGRIEQLIAVPIRAMWVGTFHGI
                     AHRMLRMHWKEAKLPQNFQILDADDAQRLVKRVLKALDLPEDQWPVKTVAGWISAQKE
                     EAKRPRDFGDTGDFSLRQMVRIYTAYQEQCERNGLVDFAELLLRAYEICRDVPEIQQH
                     YRNRFRHILVDEFQDTNRLQYEWLKVLAGRSGVLFAVGDDDQSIYSWRGARVENMMRL
                     EKDFPGCEIVKLEQNYRSTSTILKAANGLIGRNSGRLGKNLWTDGGEGEPIQLYAAFN
                     EYDEADFVAARIKEHLEGRCRHRDLAILYRMGAQSRVLEETLIRERIPYRIYGGLRFF
                     ERMEVKDALAWLRLLHSRDDDVSFERAIGAPPRGVGAGTLDKLRLFARDQNISMWAAA
                     RPGAASLGRTATPVLAFMDLIEKTAAEAQGQPLSRVMSLVIERAGLRPHYQKEKGEQA
                     EARLENLDELINAARSFENLPEEESGGDPLATFLAHASLEAGEGQAGAGEDCVQLMTL
                     HAAKGLEFPVVFLVGMENGLFPHQRAVGEGNLEEERRLCYVGITRARRKLYLSYAEMR
                     RLHGSEQLGMPSQFLKEIPAETIVETRPRIRVQRPAFSARESGRYEGTRYGGSAPEQR
                     QTPRNYSAVSGLREALPGGFRLGQRVSHAKFGEGTILNFDGNDERARVEVRFASAGTK
                     WLMLAMANLQAL"
     gene            4764..5402
                     /locus_tag="VHE37_00940"
     CDS             4764..5402
                     /locus_tag="VHE37_00940"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34119.1"
                     /translation="MAPPDTKQKLVYTALQLFAEKGYGSTSVADVRQAADIHSGSLYH
                     FFPGKQDLLLAVLQAYRDGIGPMLLDPAWRGVKDPVEKIFALLARYRASLVQTECTYG
                     CPIGSLALELHEPDPPVRELLAKNFDGWIAAIGQCLDEAGDRLPAEIDRRELAGFVLT
                     TMEGAVMQARTYRDIARFDGSVRQLRRYFDSLLRAGPSRAKPRARKTRSKRK"
     gene            5399..5941
                     /locus_tag="VHE37_00945"
     CDS             5399..5941
                     /locus_tag="VHE37_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34120.1"
                     /translation="MKTTLGVCLLLLCATAFAVDAPPSPPSPPSPPSPPSPFAAMAYL
                     AGACWQGALPGGKDSDTHCFSWVYGGKFLRDRHTVHGAGHEDYIGETIYYYDGVARKL
                     QYLYIENAGGHSSGTVESSADALVFPDARYQEDGEEQVYRSRWQKSGADAYDVKTEFR
                     QKDQWVPGFSGHMQRVDARP"
     gene            complement(6091..>6564)
                     /locus_tag="VHE37_00950"
     CDS             complement(6091..>6564)
                     /locus_tag="VHE37_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT34121.1"
                     /translation="VDGDGVPDIYVSDWQHAALGPSTGRVYLYSGKTGRRLLRLTGET
                     AGEGFGTTHASAGDIDGDGHADLAVGSWQYGGAAVSGGRVILYSGKSGKPLATYTCRI
                     AGDTFGFDAVGIGDVDGDGIDDLLITSGWSAVSGHHSGRVFIVSSGVKKIARPKP"
BASE COUNT         1169 a         2209 c         2197 g          989 t
ORIGIN      
        1 cgatacatgc gcgcgtagtc gtcgcggtag aggtcgtaca tgtgcgccga ttgccagcca
       61 tacaggccgg cgccgaccag cagcagcgcg ccgccgacgg tggccagcga ccgccagatc
      121 gacgacggcg gctcgcggtt ccagtgcagc cacgacagca gcaggaacgc gtcgaggctg
      181 gcgccgaagc gcgcggaggt gcccagcacc agcaggaaca cgtacgagaa cagcagggcc
      241 agttcggcca gctcaagctt gcgatggcgc caggcgcgcg ccgccatcag ggtgacgccg
      301 atggaagcca gcagcaggtt gagccagccg agcagccgca ccgacttgcc gtgcgaaccc
      361 ggcgccatgt aatcgacgaa gtacgggtac tggtcgacgt gcgacagcat gcgcgccagc
      421 acgccggcat tatgtccact gacgtagacg ccgccgacga tgccgtggaa tgccgccagc
      481 cgcagcagcg cccacgccgc cagcggggcc gccagcagca gcaccgggcg caggcgctgc
      541 ggcgagggtg ccgccaacca tgccagcgcg gcgtgcacca cgaaggcggc tgccaccggc
      601 accgcggttt cttttgccag cacggcgagc gccagccaca ccatcgccca gatgtaacgc
      661 tgtcgctggt aggccaggta ggcgcccagg caggccgccg ccgcgagggc atcgaacatg
      721 aagcagggca cgaacagcag cgcaatggac ccggcgaagc ccggcagcag gggcagcagc
      781 aaggccagcg gcagggcggt ggcgggctgg cgctgcgcgc cggcggcata gaccagcccg
      841 gcgcaggcgg cgatggccgc cgccgtgaca tacaagtact ggcccgagcc cacacccagc
      901 agcgcgccca gcagcaggta caccgcgttg aacaccggcc gcaggaactg catcggcggc
      961 gtgtccacgc cgggccagct ggtgaagtag cgggaaaagc cgtcgcggaa ccagccggcg
     1021 acttccccgc cgctggcggt cgagtgctgc agcagcgccc actcgtccga caccgggtgg
     1081 tacgccacga tggtgggatg catcagcagg accagcagca gcagccacag cagcgacacc
     1141 atcgctgcgc gcagcgcgat ggaggcggtg agcgccgggt gctccgcgat gcggccgccg
     1201 gccgcagagc ctgctgagat gatccccacg atgatcgttc tggcggcgcg ctcacgccca
     1261 cggcggcggc gccatcctgc tccgtaatgc tacggcgcca ccttagcggc agcacggcgc
     1321 cggcgagcac gggaatcgac ggttgcgctt gtaggaaaac tccgcggttt atgaaacctg
     1381 acgaggcatt cagcgcagca gctggctgct gatcatcttg cggtaatgcg cgccctgcat
     1441 gaagcggcgc atcaccatca ggctcttggc gtcgcggccg acaacgtagc gcagcgtgcc
     1501 gctgcggtcg ttggccgcct tccagatggc ttcggccacc agccgcgcgg gcgcgccgcg
     1561 gccgccggcc ttggccatgc ggcgcatgcc cttgtcgacg atgtcgttgt actcggccag
     1621 cgaccggtcg tggacgaaat ccatcgagcg ctcgtagaaa tcggtcttga tcgcgcccgg
     1681 ctcgatgatc ttcacccgca cgccgacatg ctccagctcg aacgccagcg attcggaaaa
     1741 cccgtccacc gcccacttgg tggcgtggta cacgctgtag aacgggaagg tgaggcgtcc
     1801 gcccatgctg gccacgttga ccaccgtgcc gccgcggttg gcgcggaaat gcggcagcag
     1861 cgcgcgggtg gtgcgcatca gcccgaacac gttggtctcg aactgcttct tgatgcgctc
     1921 gtcgtccatc gactcgaagg tgccgaccag gccatagccg gcgttgttca ccagcacgtc
     1981 gatgcggccg aattttgcga tggccgcgtc gcgcgccgcg gcgatgctgg cggcgtcggt
     2041 gacgtcgagc ttggccagca gccagcgcgg atcatcgcgt tcgcccttgg gcgtgcgcat
     2101 ggtggcagcc acgttccagc cgcgctcgaa gaacatgtcg gcggcggcgg cgccgatacc
     2161 ggtcgaggcg ccggtgatga agacggtctg ggccatgctg tgctctccgt ttttttgtcg
     2221 cagcgagggc gcattgtcgc gccaagcgga taaaatttgt cgatgcaatt tcccgtgccc
     2281 gatctcgacc ccaatctcgt cccgctggtc gagaacctca accccgcgca gaaggaagcc
     2341 gtgacggccc cgccggagca ccggctggta ctggccggcg ccggcagcgg caagacgcgc
     2401 gtgctcaccc accggctggc ctggctggtc gcggccgagg gcgtgtcgcc gcactcgatc
     2461 ctcgccgtca ccttcaccaa caaggccgcg gcggaaatgc gcgggcgcat cgagcagctg
     2521 atcgccgtgc cgatccgcgc catgtgggtc ggcactttcc acggcattgc gcaccgcatg
     2581 ttgcgcatgc actggaaaga ggcgaagctg ccgcagaatt tccagatcct cgatgccgac
     2641 gatgcgcagc gcctggtcaa gcgcgtgctc aaggcgctgg atttgcccga ggaccagtgg
     2701 ccggtgaaga ccgtggccgg ctggatcagc gcgcagaagg aagaggccaa acgtccgcgc
     2761 gatttcggcg ataccggcga tttctcgctg cgccagatgg tgcgcatcta caccgcgtac
     2821 caggaacaat gcgagcgcaa cggcctggtc gactttgccg aactgctgct gcgcgcctac
     2881 gagatctgcc gcgatgtgcc ggagatccag cagcactacc ggaatcgctt ccgccacatc
     2941 ctggtcgacg agttccagga caccaaccgg ctgcagtacg agtggctcaa ggtgctggcg
     3001 ggcaggagcg gcgtgctgtt cgcggtcggc gatgacgatc agtcgatcta ttcatggaga
     3061 ggcgcgcgcg tcgagaacat gatgcggctg gagaaggatt tccccggctg cgagatcgtc
     3121 aagctggaac agaactaccg ctccacgtcc accatcctga aggcggcgaa cggattgatc
     3181 ggccgcaaca gcgggcggct gggaaaaaac ctgtggaccg acggcggcga aggcgagccg
     3241 atccagctgt acgccgcgtt caacgagtac gacgaggcgg acttcgtcgc cgcgcgcatc
     3301 aaggagcatc ttgaaggccg ctgccgccac cgcgatctcg ccatcctcta ccgcatgggt
     3361 gcgcagtcgc gcgtgctgga agaaacgctg atccgcgagc gcatccccta ccgcatctac
     3421 ggcggcctgc gcttcttcga gcgcatggaa gtgaaggacg cgctggcctg gctgcgcctg
     3481 ctgcattcgc gcgacgacga tgtctccttc gagcgcgcca tcggcgcgcc gccgcgcggc
     3541 gtcggcgccg gcacgctgga caagctgcgc ctgttcgcgc gcgaccagaa catctccatg
     3601 tgggcggccg cgcgcccggg cgcggcgtcg ctcggccgca ccgccacgcc ggtgctggcg
     3661 ttcatggacc tgatcgagaa gaccgcggcc gaggcccagg gccagccgct gtcgcgcgtg
     3721 atgtcgctgg tgatcgagcg cgcgggtctg cgcccgcatt atcagaagga aaaaggcgag
     3781 caggccgagg cgcgcctgga aaacctggac gaactgatca acgccgcgcg cagcttcgag
     3841 aacctgccgg aggaagagag cggcggcgat ccgctggcca cgttcctggc gcacgcctcg
     3901 ctcgaagccg gcgaaggcca ggccggcgcc ggcgaggact gcgtgcagct gatgacgctg
     3961 cacgcggcca agggcctgga gttcccggtg gtgttcctgg tcggcatgga gaatggcctg
     4021 ttcccgcacc agcgcgcggt gggcgaaggc aacctcgagg aagagcgccg cctgtgctac
     4081 gtcggcatca cgcgcgcgcg gcgcaagctg tatctctcgt acgccgagat gcgccgcctg
     4141 cacggctccg agcaactcgg catgccctcg cagttcctca aggagatccc ggccgagacc
     4201 attgtcgaga cgcgcccgcg catccgcgtg cagcggccgg cgttctccgc gcgcgagtcc
     4261 gggcgctacg agggcacgcg ttacggcggc tcggcacccg agcaacggca gacgccgcgc
     4321 aattacagcg cggtctcggg cttgcgcgag gcgctgcctg gcggcttcag gctcggccag
     4381 cgcgtcagcc acgccaagtt cggcgaaggc acgattctca acttcgacgg caacgacgag
     4441 cgcgcgcggg tggaagtgcg ctttgccagc gccggtacca agtggctgat gctggctatg
     4501 gccaacctgc aggctctctg aacggcgcgg cggcgccagc agccgccaac cgcactggca
     4561 ttttgcttgt tattaaatat ttattgaata gtacatagat aaataattaa taacacatca
     4621 agtgcgggtg cggttacggc ccgcgtaggc ctgcagcagg gtttttcctc gcaaatgggc
     4681 gccaggcggc ctcgctgcgc cgcacacttg actgatcgat caagtcccgc ataagctggt
     4741 tgagttgatc aatcagtcaa gtcatggccc cgcccgatac caagcagaag ctggtctaca
     4801 ccgcgctgca gctgttcgcc gaaaaaggct acggctccac cagcgtggcc gacgtgcgcc
     4861 aggccgcgga catccacagc ggctcgctgt accacttctt tcccggcaag caggacctgc
     4921 tgctggcggt gctgcaggct tatcgcgacg gcatcggccc catgctgctc gacccggcct
     4981 ggcgcggcgt caaggacccg gtcgagaaga tcttcgcgct gctggcgcgc taccgcgcct
     5041 cgctggtgca gaccgagtgc acctacggct gccccatcgg cagcctggcg ctggagctgc
     5101 acgaacccga tccgccggtg cgcgaactgc tggcgaagaa tttcgatggc tggatcgccg
     5161 ccatcgggca atgcctggat gaagccggcg accgcctgcc ggcggagatc gaccggcgcg
     5221 agctcgccgg cttcgtgctc accaccatgg aaggcgcggt gatgcaggcg cgcacctacc
     5281 gcgacatcgc gcgcttcgac ggctcggtgc gccagctgcg ccgctatttc gacagcctgc
     5341 tgcgcgcagg cccatcccgc gcaaaaccac gcgccaggaa aaccaggagc aaacgaaaat
     5401 gaaaaccacc ctcggcgtct gcctgctgct gctctgcgca acggccttcg ccgtcgatgc
     5461 gccgccttcg ccgccctcac caccatcacc accatcacca ccatcaccgt ttgcagcgat
     5521 ggcgtatctc gccggcgcct gctggcaggg cgcgctgccc ggcggcaagg acagcgacac
     5581 gcattgcttc agctgggttt acggcggcaa attcctgcgc gaccgccaca ccgtgcacgg
     5641 cgccggccac gaggactaca tcggcgaaac catctactac tacgacggcg tggccaggaa
     5701 gctgcagtac ctgtacatcg agaatgccgg cggccacagc agcggcacgg tggaaagcag
     5761 cgccgatgcg ctggtgttcc ccgacgcgcg ctaccaggaa gacggcgagg agcaggtgta
     5821 ccgcagccgc tggcagaaga gcggcgccga tgcctacgac gtcaagaccg agttccggca
     5881 gaaggaccag tgggtgcccg gattctccgg gcacatgcag cgcgtcgacg ccaggccgtg
     5941 aatgttcgcg aaggttggtg atcgatttcg gctcgaccgc acccggattc cgatgttatt
     6001 aattattgtc tatttttaat ttcagacact aatttataac atgaaaaccg gcatcacggt
     6061 cggaaccgtc cggccgcggt gactgcctgg tcagggcttc ggccgcgcga ttttcttcac
     6121 gccgctggag acgatgaaca cgcgcccgga gtggtggccg ctcaccgcgc tccagcccga
     6181 agtgatcaac agatcgtcga tgccgtcgcc gtccacgtcg ccgatgccca ccgcgtcgaa
     6241 gccgaaggtg tcgccggcga tgcggcaggt gtaggtggcc agcggcttgc cgctcttgcc
     6301 ggaatacagg atcacgcgcc cgcccgatac cgccgcgccg ccgtattgcc acgagccgac
     6361 ggcaagatcg gcgtggccgt cgccgtcgat gtcgccggcg ctggcgtgcg tggtgccgaa
     6421 gccctcgccc gcggtctcgc cggtcaggcg cagcaggcgc ctgccggtct tgccggagta
     6481 gaggtagacg cggccggtcg atgggccgag cgcggcgtgc tgccagtcgg acacgtagat
     6541 gtcgggcacg ccgtcgccgt ccac
//
LOCUS       DASZZC010000039        11792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1031461,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000039 DASZZC010000000
VERSION     DASZZC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031461"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..830)
                     /gene="pgsA"
                     /locus_tag="VHE37_00955"
     CDS             complement(231..830)
                     /gene="pgsA"
                     /locus_tag="VHE37_00955"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HVT34122.1"
                     /translation="MRLTLPLWLTILRVAAIPAVLALFYVHFPHSRQWACVLYAAACI
                     TDWFDGYLARLWGQTSKFGAFLDPVADKLMVAVCLVMLLHAHSIDRAGAGVSAPEALM
                     ALLVAIIIGREITISALREWMAELGSRARVSVSWVGKVKTAFQMTSIGMMIWRDELWG
                     INWYALGYWLLFVAAALTIWSMAVYLRAAWPMMRDDAST"
     gene            complement(840..2708)
                     /gene="uvrC"
                     /locus_tag="VHE37_00960"
     CDS             complement(840..2708)
                     /gene="uvrC"
                     /locus_tag="VHE37_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HVT34123.1"
                     /translation="MQDAPFDSKAFLAQLTTQPGVYRMIGADDETLYVGKARNLKKRV
                     GSYFLRASGNPRIEAMVDQVRRIEVTVAATEDEALLLECNLIKDLAPRYNVMLRDDKS
                     YPYLKITAHEYPRITFYRGARSGPDQLYGPFASAYAVRESLQTLQKLFRLRPCSDSFF
                     SNRRRPCLQYQIKRCSAPCVRQVTPDDYARDVANAQRLLQGKEDELGRELGEQMERAS
                     ESLEFELAARLRDQIAALKRVQEQRATTGGVRDVDVIALAPHPSCSCVSVTGIRGGLN
                     LGHRSYFPRHPHHTEPAELLEAFIGQYYLERAAPPEIFVSHLPGDAEWLEHSLSARAQ
                     RRVRINVPERGARVRLLEVSAQSAAQSLSTRLAEAASMDARLLELQRALELEQMPRRM
                     ECFDISHSRGELTVASCVVFNEQGPLKAAYRKFNIEGVTPGDDYAAIRQAVARRFARV
                     KKGEVQAPDILFIDGGAGQLAAAQEALAEVAAAGIADAGDAAAMEQESEPFAGVQLVA
                     IAKGPTRRPGLEELLLPGHEQPLRLPPDSPALHLVQRIRDEAHRFAITGHRGRREKAR
                     VSSGLETIEGLGPARRRALLKSFGGLAQLKRASIDEIAKVQGVSRTLAERVYAYFH"
     gene            complement(2830..3519)
                     /locus_tag="VHE37_00965"
     CDS             complement(2830..3519)
                     /locus_tag="VHE37_00965"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HVT34124.1"
                     /translation="MSDRQSIPSILLAGLLVLLPSAYAAAVAPADGSKQRFDIYQDSK
                     KIGQLEFDFSSAGSDLVVVRRQSISVSKLLIKATLDESSTERWSGDRLVHLEAQAQLK
                     STIKDDSATLELSLGADGQLTGKTGKGPLSVPAGTSLFTLWRAASLHDGSYFNYVDGK
                     LVQVTLGKEPARDQAPEHTPADCTGMDATVADAEGHTSHALVWHKGDGSICSVRIKGP
                     GGEFDYLLAAG"
     gene            complement(3531..5828)
                     /locus_tag="VHE37_00970"
     CDS             complement(3531..5828)
                     /locus_tag="VHE37_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34125.1"
                     /translation="MRLGKGCAPALLLACMAHAARADEVGTIPVNAPEQTAAPAEAAP
                     GSSDATQRLEDVVVTATKRPQKLRDIPQSITALNADDLERRGVQNLQDIVRLVPGVNL
                     SYDAIAPRVTIRGISQSTNTSPTAGAIFGNVSFADAYLPVATLDANPFDLETVEVLKG
                     PQGTLFGSSNLNGAIRYVPKAPEMDAWQTKYYGQYTGIQGAWAPSYGAVVNAPLFGRD
                     DMALRVMAFQRESPGYVDVPKLGLTDTNEQQQKGARAMLAWRPGDWDVGVTYAYQRNH
                     SDNVAGTNSTNGQLADTAELRLSPQAQAYHLADLSLAYDFGWGKFTSESSGVYKAADN
                     FLEGTESVVANSPIPLASQPASEHSRTYCQEFRLASPSDASADWHWLAGALLWKEIMK
                     VDVAINIDPLQGLPLPLPPPLSLIPGGLLNDQGAVLVHALTNVSAREQALFGEVTRRF
                     FDRLEITLGGRYYRTLSGGSNVVSGLAVLTQGTTDPTSPTQATIADEIPEHGFNPKLS
                     VSLHETRNVMTYAAVSRGYRVGGVQYGVAVVPNPQSPVPRVFKSDTIWNYEGGVRTEW
                     FDKVLRFDLTGYLERWKNPQYYTIDATNTSKYLTNVGGVKSTGAEAALQFITPWGIMF
                     SNTAAYSKTVTTEIFKAANGNDAPVGSPWPYSPKWQTASTVAYLTSLGNWNLNSAITY
                     TYMGKAISDLSDMLPVYGFRQLDLALSVENGALAWLPDLELSLSNALDERAIGGQSYN
                     DTTKAHYGVVYIQPRTLNLRLVGHF"
     gene            6014..6973
                     /locus_tag="VHE37_00975"
     CDS             6014..6973
                     /locus_tag="VHE37_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34126.1"
                     /translation="MAPQPKLKGFTLRLLYAQFSLLAKLGLGVDPDKTVAQPMSVRKN
                     KAPAWMTLPLPPDVRLELDSFNGRAGPIAIKKYLPPKIDPSMPRLMFLHGGGWIGGGL
                     DTLDHLCASVAAQASCLVVSVEYRLAPETPFPGALEDCDDALSWTLRDSSLGPLPAAG
                     LVVMGESAGGNLAAALCVLRARQGNSPIRHQILIYPALDFTMASESMHTIRQPGLEKK
                     NVLRLIEMYCGQADLKDPLVSPMFADNASALPPAYIITADVDPLRDDGARYARKLADA
                     GVPVRYDNYPGMPHGFFFTPGICSSAVPAIAEIVKELRKLAKG"
     gene            7010..8011
                     /locus_tag="VHE37_00980"
     CDS             7010..8011
                     /locus_tag="VHE37_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVT34127.1"
                     /translation="MKTRTRPKSMADIAALAKVSKPTVSRALRDSPLVTPETKALILA
                     VAAKHGYAVNRNAQKLRDKRTNTIAVVADFKSFPGSRIHDPFSFELLADVGQALALRN
                     QDLLLCAPAVDENHSYESIVVSRHADGIIFLGQGRRDAALRKLADTDAPFVVWGAVAK
                     DQPYCAVGGDNLRGGELAGERFALMRRRKPLYIGGSDEHLEMKLRRQGFEKALRNAST
                     SLILADFSYEAALAALDAHLDSKTKVPDAIFAASDTIAMGVYAALKKRGLKIPDDVSV
                     IGYNDNPHAAWCTPSLTTVRQDTRQAGALLVEQLMRLLEGGSAKSIMMPAELVVRES"
     gene            complement(8134..9633)
                     /locus_tag="VHE37_00985"
     CDS             complement(8134..9633)
                     /locus_tag="VHE37_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HVT34128.1"
                     /translation="MKIFSAFFATETNTSAVVPTGFADFERIGVYRGDASARDPLGFG
                     ASHVELKRLASADGHVVVESVTAHAHPAGITVRQVYERYRDWILEDLRAAMPVQAVVL
                     ALHGAMVADGYDDCEGDLIARVREMVGPKVPIGVELDLHCHVSRLMLGKADVIVCYKE
                     YPHTDTLERLGEVYRLVVRQAQGEIKPVTAVRDLRMVGIWHTTREPMASFVKKMKSFE
                     GSGGILSVSLGHGFPWGDVADTGAKLWVIADSDQAKAQQLADQLGDEFWRLRDQTYSP
                     PLSIGAAIEKMKPAQQGPIVFADVADNAGGGAMSDSTFILKAMLDAGIADSVIGSFWD
                     LGAVELCKSAGVGARLNLRIGGKCGPQSGDPVDVAITVKAILENHAQFGLNFRWPLGT
                     CVWVQTEGGIDILLNTIRSQIFGREAFEGLGIHLEGRRAIVVKSTQHFYASFAPLAKE
                     VLYVGAPGSITQNFAEMTYRKRDNYFWPRVADPFKTPMATAASKFNDRS"
     gene            complement(9668..10291)
                     /locus_tag="VHE37_00990"
     CDS             complement(9668..10291)
                     /locus_tag="VHE37_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34129.1"
                     /translation="MSKAAILKKRARGRPRSIDRETVLAAAMELVDPDLSLPELAESL
                     DINLAAIYRIFGDKVGLYTAMYAEALRQLDRIEGTTWTSWLDSYVNVLLKLARRYPFV
                     VSLQASHKNHYVELRNLSERSVALVSPGIELLVKAGLDREMAHNALIGVKAIVYEHAL
                     TEQFYSEAPVILADFETLGGSGEQRLWKFLKVIRNGLQAEMKSAKGK"
     gene            10392..>11792
                     /locus_tag="VHE37_00995"
     CDS             10392..>11792
                     /locus_tag="VHE37_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT34130.1"
                     /translation="MKTKLRTAWTLSLLLLVSLSGVVSAQGAAENEAAPGAGIAPAPG
                     LQPDEKSIPVTDLKPEAPAPSGESEPATRLEEVVVTANKRQQSERTVAGAVTAVTRER
                     LDEVGASTFGDYLSLSPGVNFNSGTPGYSVITIRGISSDTIPTLTQTAVGTYYDDIPL
                     TDPGTPILVPDIDAFDADRIEVLRGPQGALYGSASLGGAVNYIPESPDAYNFGLHAQV
                     VGDRKKNSSLGGAGRLMANLPLPIDGAGLRVVGNYTNAPGFIDNIGLDRKQTNSSSTR
                     GGRAILRFAPTADSRLRLTALIQRTSVEDAGYVDQTLGDLKKTSTKLEPSDNQIKLGS
                     LRYEFDTGFGNWTLVGGYQDKRSSLSFDAKNALGLTATGQQLPLILDQGGNVKGYSGE
                     LRYISPEWDRFNFLAGASYANRDEIFDLNLDSQNLAEIADQLRATLAQLGLMLPGQIG
                     SAATVFREHAEIQAPET"
BASE COUNT         2066 a         3855 c         3743 g         2128 t
ORIGIN      
        1 ccagttcgat cgagcgggga tgcagctgct cctgccaggc cagccattgc gccagcgtcc
       61 agtcagcttg tggattcatc ccgcgtcatg tcgaagcgtc atcgcgcatc atcggccatg
      121 cagcgcgcag gtgcgcggcc gacacggcgc cgtgagcggg ctccagcccg cgagcgtggg
      181 tgcgggaggc cacgcgtatc tgcgcgccct ccggtgcagg aaagggaatc tcacgtggaa
      241 gcgtcatcgc gcatcatcgg ccaggcggcg cgcagataga ccgccatgct ccagatggtc
      301 agcgcggcgg cgacgaacag cagccagtag cccagcgcgt accagttgat gccccacagt
      361 tcgtcgcgcc agatcatcat gccgatcgag gtcatctgga acgcggtctt gaccttgccc
      421 acccagctca ccgacacgcg cgcgcggctg ccgagctcgg ccatccattc gcgcagcgcg
      481 gagatggtga tctcgcggcc gatgatgatg gccaccagca gcgccatcag cgcctccggc
      541 gcgctgacac cggcgccggc gcggtcgatc gagtgcgcgt gcagcagcat gaccaggcac
      601 accgccacca tcagcttgtc cgccaccggg tcgaggaagg cgccgaactt gctggtctgg
      661 ccccacaggc gcgcgagata accatcgaac cagtcggtga tgcaggcggc ggcgtagagc
      721 acgcaggccc actgccgcga gtgcggaaag tgcacgtaga acagcgccag caccgccggg
      781 atggcggcga cgcgcaggat ggtgagccac agcggcaggg tcagtcgcac ccgccgattt
      841 taatggaagt aggcgtagac ccgctctgcc agcgtgcgcg aaacaccttg aacttttgcg
      901 atctcgtcga tgctggcgcg cttgagttgc gccagcccgc cgaaactctt gagcaatgcg
      961 cggcgccgcg ccgggcccag tccctcgatg gtttccagcc ccgacgacac ccgcgccttc
     1021 tcgcgcctgc cgcgatgccc ggtgatggca aagcggtgcg cctcgtcgcg gatgcgctgc
     1081 accaggtgca acgccggcga atccggcggc aggcgcagcg gctgctcgtg cccgggcagc
     1141 agcagctcct ccaggcctgg ccgccgcgtc ggacccttgg caatggcgac cagctgaacg
     1201 ccggcgaatg gttcgctctc ctgctccatc gcggccgcat cgcctgcatc ggcaatgccg
     1261 gctgcggcga cttcggccag cgcttcctgc gcagcggcca gctggccggc gccgccgtcg
     1321 atgaacagga tgtcgggcgc ctgcacctcg cctttcttta cgcgggcgaa gcggcgggcg
     1381 acggcctgcc tgatcgcggc gtagtcgtcg ccgggcgtca cgccctcgat gttgaacttg
     1441 cggtacgccg ccttcagcgg cccctgctcg ttgaacacca cgcacgaggc cacggtgagc
     1501 tcgccgcggc tgtgcgagat gtcgaagcac tccatgcgcc gcggcatctg ttctagctcc
     1561 agcgcgcgct gcagttcgag caggcgcgcg tccatgctcg ccgcctcggc caggcgcgtg
     1621 gacagcgact gcgccgcgct ctgcgccgac acttccagca agcgcacgcg tgcgccgcgc
     1681 tcgggcacgt tgatgcgtac gcgacgctgc gcccgcgcgg acagtgagtg ctccagccac
     1741 tcggcgtcac cgggcaaatg gctgacgaag atttccggcg gcgccgcgcg ctccaggtag
     1801 tactggccga tgaaggcctc cagcagttct gccggctcgg tgtggtgcgg gtgcctgggg
     1861 aaatagctgc ggtgccccag gttgagcccg ccgcggatgc cggtgacgct gacgcagctg
     1921 cacgatggat gcggcgccag cgcgatcacg tccacgtcgc gcacgccgcc ggtggtggcg
     1981 cgctgttcct gcacccgctt gagcgccgcg atctggtcgc gcaggcgcgc cgccagctcg
     2041 aattccagtg attcgctggc gcgctccatc tgctcgccca gctcccggcc cagttcatcc
     2101 tccttgccct gcagcaggcg ctgcgcattg gccacgtcgc gcgcgtagtc gtccggcgtc
     2161 acctggcgca cgcacggcgc cgagcagcgc ttgatctggt actgcaggca tggccgccgg
     2221 cggttggaga aaaagctgtc gctgcacggg cgcaggcgga acagcttctg cagcgtctgc
     2281 agcgattcgc gcaccgcgta ggcgctggcg aaaggcccat agagctggtc gggcccgctg
     2341 cgcgcgccgc ggtagaaggt gatgcgtgga tactcgtgcg cggtgatctt caggtacggg
     2401 tagctcttgt cgtcgcgcag catcacgttg tagcgcggcg ccaggtcctt gatcaggttg
     2461 cactcgagca gcagcgcctc gtcttcggtc gctgcgaccg tgacctcgat gcggcggacc
     2521 tggtcgacca tcgcctcgat gcgcgggttg ccgctggcgc gcaggaagta gctgccgacg
     2581 cgcttcttca ggttgcgcgc cttgccgacg taaagtgtct cgtcgtcggc gccgatcatg
     2641 cggtacacgc cgggctgcgt ggtcagttgc gcgagaaagg ccttggagtc gaacggagcg
     2701 tcctgcatgg ccaattttgc ttgaccgcac tctggttttg tgtgttatta aatgatgtat
     2761 atttttataa ttgttaaata aataataaca cacaaaaacg gcatgcggtg gagggtccag
     2821 gcagcgtttt cacccggcag ccagcaggta gtcgaactct cctccagggc ccttgatgcg
     2881 cacggagcag atcgagccgt cgcccttgtg ccagaccagc gcgtgcgagg tatggccttc
     2941 cgcgtcggcc accgttgcgt ccatgcccgt gcaatccgcc ggtgtatgtt ccggcgcctg
     3001 gtcccgcgcc ggctcttttc cgagcgtgac ctgcaccagc ttcccgtcga cgtagttgaa
     3061 atagctgccg tcgtgcagcg atgccgcccg ccacaaggtg aacagcgagg tgcccgcagg
     3121 aaccgacagc ggccccttgc ccgtcttgcc ggtcagctgg ccatcggcgc cgagcgacag
     3181 ctccagcgtg gcgctgtcgt ccttgatggt ggacttgagc tgggcctgcg cttcgagatg
     3241 caccaggcga tcgcccgacc agcgttccgt gctgctctcg tcgagcgtcg ccttgatcag
     3301 cagcttgctg acgctgatcg actggcggcg caccaccacc agatcgctgc ccgcagacga
     3361 gaaatcgaat tcaagctggc cgatcttctt gctgtcctgg tagatgtcga accgctgctt
     3421 cgaaccgtcc gccggcgcca cggctgctgc gtacgctgac ggcaacagca cgagcaggcc
     3481 ggccagcagg atggaaggaa tggactgacg gtcggacatg ggctgcacgc tcagaaatgc
     3541 cccaccaggc gcaggttcag cgtgcgcggc tggatgtaga cgacgccgta gtgcgccttg
     3601 gtcgtgtcgt tgtagctctg gccgccgatg gcgcgctcgt ccagcgcatt gctcagcgaa
     3661 agctcgaggt ccggcagcca ggccagcgcg ccgttttcca cgctgagcgc caggtccagc
     3721 tggcggaagc cgtagaccgg cagcatgtcg ctcagatcgg aaatcgcctt gcccatgtac
     3781 gtgtacgtga tggcgctgtt caggttccag ttgcccaggc tggtgaggta ggcgacggtg
     3841 ctggcggtct gccacttcgg cgaatacggc caaggagaac cgaccggcgc atcgttgccg
     3901 ttggccgcct tgaagatctc ggtggtgacg gtttttgaat acgccgccgt gttgctgaac
     3961 atgatgcccc acggcgtgat gaactgcagc gccgcttccg cgccggtgct tttcacgccg
     4021 ccgacgttgg tcaggtattt gctcgtgttg gtcgcgtcga tggtgtagta ctgcgggttc
     4081 ttccagcgtt cgaggtagcc ggtgaggtcg aagcgcagca ccttgtcgaa ccactcggtg
     4141 cgcacgccgc cctcgtaatt ccagatggtg tcggacttga acaccctggg cacggggctt
     4201 tgcggattcg gcacgacggc aacgccgtat tgcacgccgc ccacgcggta gccgcgcgag
     4261 accgcggcat acgtcatgac gttcctggtc tcgtgcagcg acaccgacag cttggggttg
     4321 aagccatgtt ccggtatctc gtcggcgatg gtggcctgcg tgggcgaggt cggatcggtc
     4381 gtgccctggg tgagcaccgc caggcccgac accacgttgc tgccgcccga gagcgtgcgg
     4441 tagtagcggc cgccgagggt gatttccagc cggtcgaaga agcgccgcgt gacttcgcca
     4501 aacagcgcct gctcgcgcgc gctgacattg gtcagggcat gcaccagcac cgcgccctgg
     4561 tcgttgagca ggccgcccgg gatcaggctg agcggtggcg gcaagggcag cggcagtccc
     4621 tgcagcggat cgatattgat cgccacgtcg accttcatga tctccttcca cagcagcgca
     4681 ccggccagcc agtgccagtc cgccgaggcg tctgacggcg aggccaggcg gaattcctgg
     4741 caataggtgc gcgaatgctc ggacgccggt tgcgatgcga gcgggatcgg tgagttggcc
     4801 accacgctct cggtgccttc caggaagttg tccgcggcct tgtacacacc cgagctttcc
     4861 gacgtgaact tgccccagcc gaagtcatag gccagcgaga ggtcggccag gtgataagcc
     4921 tgcgcctgcg ggctcagccg cagctccgcg gtgtcggcca gctggccgtt ggtgctgttg
     4981 gtgccggcga cgttgtcgct atggttgcgc tggtaggcgt aggtgacccc gacgtcccag
     5041 tctcccggcc tccaggccag catcgcgcgt gcgcccttct gctgctgctc gttggtatcg
     5101 gtgaggccca gcttgggcac atcgacgtag cccggcgact cgcgctggaa cgccatgacc
     5161 cgcaacgcca tgtcgtcgcg gccaaacagc ggcgcattga ccaccgcgcc gtacgacggc
     5221 gcccacgcac cctggatgcc ggtgtactgg ccgtagtact tggtctgcca ggcatccatc
     5281 tccggcgcct tgggcacgta gcggatcgcg ccgttgaggt tagacgagcc gaacagcgtg
     5341 ccctgcggac ccttcagcac ttccaccgtt tcgagatcga acgggttggc gtcgagcgtg
     5401 gccaccggca ggtacgcgtc ggcgaatgac acgttgccga agatggcgcc ggcggtcggg
     5461 ctggtgttgg tgctttggga aatgccgcgg atggtcacgc gcggcgcgat ggcgtcgtag
     5521 gaaagattga cgccgggcac cagcctcacg atgtcctgca ggttctgcac gccgcgccgt
     5581 tcgaggtcgt cggcgttcag cgcggtgatg ctctggggaa tgtcgcgcag cttctgcggg
     5641 cgcttggtcg cggtgaccac cacgtcttcc aggcgttgcg tggcgtcgct cgacccgggc
     5701 gcggcttctg ccggcgccgc ggtctgctcc ggtgcattca ccggtatggt ccccacctca
     5761 tcggcccgtg ccgcgtgcgc catgcacgcc agcagcagcg ccggtgcgca gcccttgccc
     5821 agtctcattg gaattctcct ccctgcacca ctgccacgat cttcggtctg cagccgatct
     5881 cgaggcacat ctgtttgatc gcagaataca tggttatgaa atgcatttca aaaaatgacc
     5941 ggattgatat actgccggcg cgcctggctg cggccacgac gcctgcgaag tcaataacgg
     6001 gggagttcga gtgatggcac cgcaacccaa gctcaagggg ttcaccctgc gcctgctcta
     6061 cgcgcagttc agcctgcttg ccaagctcgg cctgggcgtc gaccccgaca aaaccgtggc
     6121 gcagccgatg tcggtgcgca agaacaaggc gcccgcctgg atgacgctgc cgctgccgcc
     6181 ggacgtgcgc ctggaactcg acagcttcaa cggccgcgcc gggccgatcg caatcaagaa
     6241 atacctgccg ccgaagatcg atccgtccat gccgcggctg atgttcctcc atggcggcgg
     6301 ctggatcggc ggcgggctcg acacgctcga ccacctctgc gccagcgtcg ctgcccaggc
     6361 gtcgtgcctg gtggtgtcgg tggaataccg gctggcgccg gagacgccct tccccggcgc
     6421 gctggaagac tgcgacgacg cgctgagctg gaccttgcgc gattcctcgc tcggcccgct
     6481 gcccgctgcg ggcctggtgg tgatgggcga aagcgccggt ggcaacctcg ccgccgcgct
     6541 gtgcgtgctg cgcgcgcgcc agggcaactc tccgatccgt caccagatcc tgatctaccc
     6601 cgcactcgat ttcaccatgg cgtcggaatc gatgcacacc atccggcagc cggggctgga
     6661 aaagaaaaac gtgctgcgct tgatcgagat gtactgcggc caggcagacc tgaaagaccc
     6721 gctggtgtcg ccgatgtttg cagacaacgc ttcggcgctg ccgccggcct acatcatcac
     6781 cgccgatgtc gatccgctgc gcgatgacgg cgcgcgctat gcgcgtaagc tcgccgacgc
     6841 cggcgtgccg gtgcgctacg acaactaccc cggcatgccg cacggcttct ttttcacgcc
     6901 ggggatctgc tcgtcggcgg tgcccgccat cgccgaaatc gtcaaggaac tgcgcaagct
     6961 ggccaagggc tgatgaagcc cgcggttcat cgcaatttat aattggcgca tgaaaaccag
     7021 aacgcgcccg aagagcatgg cggacatcgc ggcgctggct aaagtatcca agcccacggt
     7081 gtcgcgcgcg ctgcgcgata gcccgctggt cacgcccgaa accaaggcgc tgatcctggc
     7141 ggttgccgcc aagcatggct atgccgtcaa ccgcaatgcg cagaagctgc gcgataagcg
     7201 caccaacacc attgccgttg tcgccgactt caaatcgttt cccggcagcc gcatccacga
     7261 tccgttcagc ttcgaactgc tggccgatgt cggccaggcg ctggcgctgc gcaaccagga
     7321 cctgttgctg tgcgcacccg cggtcgacga gaaccactcc tacgaatcga tcgtcgtgtc
     7381 gcgccacgcc gacggcatca tcttcctggg ccagggccgc cgcgacgccg cgctgcgcaa
     7441 gctcgctgac accgacgcgc ccttcgtggt gtggggcgcg gtggcgaaag atcagcctta
     7501 ctgcgccgtc ggcggcgaca acctgcgcgg cggcgaactc gccggcgagc gcttcgcgct
     7561 gatgcgccgg cgcaagccac tgtacatcgg cggcagcgac gagcacctgg agatgaagct
     7621 gcgccgccaa ggcttcgaga aagccctgcg caacgcgtca acctcgctga tcctcgccga
     7681 cttctcctac gaagccgccc tggccgcgct cgacgcgcac ctcgattcca agacgaaggt
     7741 gccggacgcg attttcgctg ccagcgacac catcgccatg ggcgtctatg ccgcgctgaa
     7801 aaaacgcgga ctgaagattc cagacgacgt ctcggtgatc ggctacaacg acaacccgca
     7861 cgccgcctgg tgcacaccat cgctcaccac cgtgcgccag gacacgcgcc aggccggcgc
     7921 gctgctggtc gagcagctaa tgcgtttact cgaaggcggc agcgcaaagt ccatcatgat
     7981 gccggcggag cttgtggttc gagaatcgtg atcaggctac tgtgctgatc gacggcaagc
     8041 agcccgaagc ggtcccacgg cgaaactcgc tcagcggcgc tcatcggcca ggtgcggaca
     8101 ccgggttcac gtacccggat gtccgatgtt cgcctatgag cggtcgttga atttcgaagc
     8161 agccgtggcc attggcgttt tgaacggatc tgccacccgg ggccagaaat agttatcgcg
     8221 cttgcggtag gtcatttcgg cgaaattctg ggtgatcgag cccggcgcgc ccacgtaaag
     8281 cacctccttt gcgagcggtg cgaaggatgc ataaaagtgc tgggtggact tcaccacgat
     8341 tgcccggcgg ccctcaaggt gtatgccgag accttcaaac gcctcgcgac caaagatctg
     8401 cgagcggatc gtattgagca ggatgtcgat accaccctcg gtctgcaccc atacgcaggt
     8461 acccagaggc cagcggaagt tgagcccgaa ctgggcgtgg ttctcgagta tggccttgac
     8521 cgtgattgca acgtccaccg ggtcgcccga ttgggggccg cacttgccgc cgatgcgcaa
     8581 gttcagccgc gcgccgaccc ccgcgctctt gcagagttcc actgcgccca agtcccagaa
     8641 actgccgatg accgaatccg ctatgccggc atcgagcatg gccttgagga tgaaggtgct
     8701 gtcactcatg gcgccgccgc cggcgttgtc tgcaacgtcc gcaaacacta tcggaccctg
     8761 ctgcgccggc ttcatcttct ctatggcagc gccgatggac agcggcggtg agtatgtctg
     8821 atcgcgcagc cgccagaact catcccccag ctgatcggcc agctgctggg ctttggcctg
     8881 gtcgctgtcg gcaatcaccc agagtttcgc accggtatcc gccacgtcac cccaggggaa
     8941 accatgcccg agcgagacgg agaggatccc gcccgagccc tcgaaagact tcatcttctt
     9001 tacgaaggag gccatgggct cgcgcgtggt atgccagatg ccaaccatgc ggaggtctcg
     9061 caccgccgtc accggcttga tctcgccctg cgcctgccgc accaccaaac ggtagacctc
     9121 gcccaggcgt tccaacgtgt cggtgtgcgg gtattccttg tagcaaacga tgacgtcggc
     9181 ttttcccagc atcagtcggc tgacgtggca gtgcaaatca agctcgacgc cgataggcac
     9241 cttggggcca accatctcgc gaacgcgcgc gatgaggtcg ccttcgcagt cgtcgtagcc
     9301 gtcggccacc atggcgccgt gcagtgccag gacaacggcc tgcaccggca tcgccgctct
     9361 caggtcttcc agaatccagt cccggtaacg ctcatacacc tgtcgcacgg tgatgcccgc
     9421 cggatgcgca tgggcagtca cgctctccac cacgacatgg ccgtcagccg aggcgaggcg
     9481 cttgagttcg acgtgggatg cgccaaagcc cagagggtcg cgtgcgctgg cgtcgccgcg
     9541 atagacaccg atgcgctcaa agtcggcaaa tcccgtaggg acgaccgccg aggtgttggt
     9601 ttcggtggcg aaaaaagcac tgaagatttt catcgtcaat tcacgtcacc acgatatggc
     9661 attgctgcta cttgcccttg gcgcttttca tttcagcctg gaggccgttg cggataacct
     9721 tcaagaactt ccagagccgc tgctcgcctg agccgcccaa agtttcaaaa tccgcaagga
     9781 tgactggagc ttccgaataa aactgctcgg taagagcatg ctcgtacacg atggccttca
     9841 cgccgatgag cgcgttgtga gccatttcgc ggtcgaggcc tgccttcacc aagagttcga
     9901 ttccaggact caccagggcc acggaccgct cagacagatt gcgcagttcg acatagtggt
     9961 tcttgtggct ggcctgcagg ctgacgacga acgggtaccg tcgcgccagc ttcagcaaca
    10021 cgttgacgta gctatcgagc caagacgtcc aggtcgtgcc ctcgatgcgg tccagttgcc
    10081 gcagcgcctc tgcatacatg gcggtataca aacccacttt gtcgccaaag atgcggtaga
    10141 tggccgccag gttgatgtcg agcgactcag ctagctcagg caggctcaga tcggggtcga
    10201 cgagttccat cgctgcggcc agaacggttt cgcgatcaat gctccgcggc cgaccgcggg
    10261 cccgtttctt caatatcgca gctttgctca tctcgaatat ccgctctata tgtcgccgtt
    10321 gacagcacca cttcgcgaat aatagcatcc gtatttccga aaatcttttc gaaaatataa
    10381 tcggggcgct cgtgaagacc aaactgagaa ccgcatggac gctgtcgctg ctcttgttgg
    10441 tgtcactctc tggcgttgtc tcagcgcaag gcgcagctga aaatgaggcg gcgcccggag
    10501 caggtatagc tcccgcccct gggctgcagc cagatgaaaa gtcgattcca gtaacggacc
    10561 tcaagccaga agcgccggct ccatcgggtg aaagtgagcc tgcgacgcga ctggaggaag
    10621 tggtggtcac ggcaaacaag cgccagcaga gcgagcgcac cgtggcgggt gccgtcaccg
    10681 cggtcacgcg tgaacgtctc gacgaggtcg gcgcctcgac cttcggcgat tacctgtcgt
    10741 tgtcaccggg cgtgaatttc aacagcggca cgccgggtta ctccgtcatc accatccgag
    10801 gtatctcgtc ggacacgatt cccacgctta cgcaaaccgc ggtcggcacc tactacgatg
    10861 acattccgtt gaccgatccg ggcacgccga tcctggtgcc ggacattgac gcctttgatg
    10921 ccgatcgcat cgaggtgctg cgtggcccgc aaggtgcact gtacggttcg gcgtcccttg
    10981 gcggcgcggt gaactacatt ccagaatcac ccgacgccta caactttggc ctgcacgctc
    11041 aagtcgtcgg cgaccggaag aagaacagct cgttgggcgg agccggccgt ctcatggcga
    11101 atcttccgct gcccattgat ggcgcgggcc tgcgtgtcgt cggaaattac acgaatgcgc
    11161 cgggcttcat cgacaacatc ggactggatc gcaagcagac caattcctcg agcactcgcg
    11221 gcggacgcgc gattcttcgt ttcgcgccaa cggctgactc gcgccttcgc ctcacggcgc
    11281 tgatacagcg cacatccgtt gaagacgcgg gctacgtgga ccagaccctc ggcgatctga
    11341 aaaagaccag cacgaagctc gaacccagcg ataaccaaat aaaactcggg tcgttacgtt
    11401 acgagttcga caccgggttc ggtaactgga cccttgtcgg aggctaccag gacaagagga
    11461 gcagcctgag ttttgacgcc aagaatgcgt tagggctgac ggcgaccggt cagcagttgc
    11521 ccctcatctt ggatcaggga ggcaacgtca aaggctacag cggggaactg cgttacatct
    11581 cgccggaatg ggatcgtttt aacttccttg ccggcgcctc gtacgccaac cgggacgaga
    11641 ttttcgatct gaacctcgat tcgcagaacc tggcagagat cgccgaccag ctgcgcgcga
    11701 cgctggcgca gctcggcctg atgctgcctg gccagatcgg ctctgcagca acggtgttcc
    11761 gcgagcacgc tgaaattcag gcgccggaaa cc
//
LOCUS       DASZZC010000040         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_965459,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000040 DASZZC010000000
VERSION     DASZZC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965459"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VHE37_01000"
     CDS             complement(<1..523)
                     /locus_tag="VHE37_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34131.1"
                     /translation="MENEWQLGRWWRVPLAMHWTVLLMLPWMFLWYRDVAITVGAFTG
                     YFLVLLAHEFGHVWMARRLRIPVEGILLVGIHGQTSHGYANRADDLKIAWSGVGSQLL
                     ILLAAWLTTPLVLRLQLPLLDRFVGGLWLGFTDINLFLVVAALLPIGPFDGHRAWQVI
                     PYWRDRFRRRRRGR"
     gene            complement(580..1089)
                     /locus_tag="VHE37_01005"
     CDS             complement(580..1089)
                     /locus_tag="VHE37_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HVT34132.1"
                     /translation="MSPRLLRFLRHASTGQRAVQRAFPADAMEQIAQAVRDCEARHAG
                     EIRFAVESSLPLDAAWHGVTARERALDVFSQLCVWDTEHNNGVLIYLLLADRAVEIVA
                     DRGVARERVAPSEWDAACRAMEQHLRDGQYLDAVRSGIAAVADVLARHPPQFPDAGNE
                     LPDAPVVLR"
     gene            complement(1091..>1522)
                     /locus_tag="VHE37_01010"
     CDS             complement(1091..>1522)
                     /locus_tag="VHE37_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HVT34133.1"
                     /translation="DFYGGIDAGVSQMMQVIQGEPLPPPRARRGTSDDDRLGSLLVPF
                     FIFWTVGHVLKRLFGTFPASALVGIGTGIASVFILGFGILAIGAGFLAMIAALVLYSG
                     ALAGLPGRGGYYGGGGGGGFGGGGFSGGGGGFGGGGASGRW"
BASE COUNT          275 a          554 c          508 g          185 t
ORIGIN      
        1 cgcggccgcg gcggcgccgg cgaaaccggt cacgccagta cgggatcacc tgccaggcgc
       61 ggtgaccgtc gaacgggccg atcggcagca gtgccgccac caccaggaac aggttgatgt
      121 cggtgaagcc cagccacagg ccgccgacga agcggtcgag caagggcagt tgcaggcgca
      181 gcaccagcgg cgtggtcagc cacgcggcca gcaggatgag caactgcgag ccgacgccgc
      241 tccaggcaat tttcaggtcg tccgcccggt tggcgtagcc atgcgaggtc tgcccatgga
      301 tgccgaccag caggatgcct tcgaccggga tgcgcaggcg ccgcgccatc cacacgtggc
      361 caaactcatg cgccagcagc accaggaaat acccggtgaa ggcgcccacg gtgatggcga
      421 cgtcgcggta ccagaggaac atccacggca gcatcagcag caccgtccag tgcatcgcca
      481 gcggcacgcg ccaccagcgg cccagctgcc attcgttttc catggaccaa gcttagcgcg
      541 ccggccggct gctgcgccct ggcgcagcgt taacggtcgt cagcgcagca ccaccggtgc
      601 gtccggcagc tcgttgccgg catcggggaa ctgcggcggg tggcgcgcca gcacgtcggc
      661 cacggcagcg atgccggaac gcacagcgtc caggtactgt ccatcacgca ggtgttgttc
      721 catggcgcgg caggctgcat cccactcgga tggggccacg cgctcgcgcg ccaccccgcg
      781 gtcggccacg atctccaccg cgcggtcggc cagcagcagg tagatcagca cgccgttgtt
      841 gtgctcggtg tcccacacgc acaactggga aaagacgtcc agcgcacgct cgcgcgcagt
      901 aacgccgtgc caggccgcat cgagcggcag cgacgactcc accgcgaagc ggatttcgcc
      961 ggcatggcgc gcttcgcagt cgcgcaccgc ctgtgcgatc tgctccatcg catcggccgg
     1021 aaaggcgcgt tgcacggcac gctggccggt gctggcgtgg cgcaggaacc gcagcaggcg
     1081 cgggctcatg tcaccagcgc cccgacgcac cgccgccgcc gaatccacca ccgccgccgg
     1141 aaaatccgcc gccaccgaat ccgcctcccc caccaccgcc gtagtagccg ccgcggccgg
     1201 gcaggccggc caacgcgccg ctgtacagca ccagcgcggc gatcatcgcg aggaatcctg
     1261 caccgatcgc caggatgccg aaacccagga tgaacaccga cgcgatgccg gtgccgatgc
     1321 ccaccagggc ggacgccggg aaggtgccga acagtcgctt gagtacatgg ccgacggtcc
     1381 agaagatgaa gaacggcacc agcagcgagc ccagccggtc gtcgtcgctg gtgccgcggc
     1441 gcgcgcgcgg cggtggcagc ggctcgccct ggatcacctg catcatctgg ctgacgccgg
     1501 cgtcgatgcc gccgtagaaa tc
//
LOCUS       DASZZC010000041         9117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_903057,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000041 DASZZC010000000
VERSION     DASZZC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903057"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VHE37_01015"
     CDS             <1..1102
                     /locus_tag="VHE37_01015"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HVT34134.1"
                     /translation="ERDGLLNRTIEFLPDDESLKERRSHGRGLTRPELSVLLAYSKIS
                     LKDAMLKSQVPDDPYFERDLLANFPPLLVKKFGEVLVHHQLKREIVATMLTNAVVNRM
                     GVSFSHRLAEDHGQPRAQIIKAYAAAHQIYDADRYWTAIESLDNKVKASLQYALYERA
                     IGLLKHVTGWIVNSDMAQAPIGAIVARFAAAIPAGEKLLPDALPASYRADWDKAVDSM
                     VREGVPQELAVMLANTKVLGSGPDVVELAEAAKAPLTETAQVYFEIGDRFSVLWLLSA
                     IIGLSVQSKWQALARANLRDDVYRLQRRLASQALRAPGKTAQAKVEAWMAANEEVVSF
                     ARRRLNELQTGGAADFMSLAVAVRALRDLRTL"
     gene            complement(1112..2617)
                     /locus_tag="VHE37_01020"
     CDS             complement(1112..2617)
                     /locus_tag="VHE37_01020"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HVT34135.1"
                     /translation="MNKSSAAKQKNGALKLHQVTNPLCTAGKAAPEVQVLLVGQDRQL
                     RTAGRELPALPASFRGTTGERIEISAAKARLLVVGCGKMPATAEEPMYWESAGAAVID
                     AMRSLKIARAALSGDLVPAAIDAAAAARGFAIGATLASYRCVAYRRQPPKDHFNVESL
                     ALSAADWKAGMRGRELGDAVNWARALVDAPANLLHPQTFAAEIASLKSFGIEVEVLDL
                     KALERIGAGGLLAVGRGSVHPPCMVICRWQGRSSKENDLGLVGKGLTFDAGGLNIKMP
                     PGIGKMKFDMGGAAAVVGALRVMAQRKAPVNVVAALPLCENLIDADSYRPGDVITSLS
                     GLTIEVDNTDAEGRIVLADGISHLINKFRPRLLVDVATLTGSIMASLHEEYAGLFSTD
                     EALAAALGEAGASQGEDLWRMPLSKKQDYIVDSEIADVKNLGAPGFFGTSAGSAIAGA
                     KFLERFAAGTRWAHLDIAGTVWITRPKPGIPKGATGFGVRLLDALADRLPA"
     gene            complement(2628..3515)
                     /locus_tag="VHE37_01025"
     CDS             complement(2628..3515)
                     /locus_tag="VHE37_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT34136.1"
                     /translation="MTTREDIAEIPGAELRYRLAGTPGTLPTIVLENGWGASYDYFAL
                     LQEALAPHTQLLLYNRAGIGGSMAHETLTAEGMSRHLAALLDALGIRAPVVIAGQSYG
                     GLICGVHAALMPQRLRAVVQIDPTAERTDEQIDATLGSMRGLGKVLRLLAALRIPEPF
                     FAPHMPELPAADTAQLRRNAFGNAASLRAAGIELDLLQDIRRVCAQPSPVPRLVISAG
                     TTEEVRNALFRLLVSPERARSLLGRMQAQHEITAGRGGPGSRWITLPHTHGALVVSRS
                     GAAATATCMIDYLSSLPTT"
     gene            complement(3523..5871)
                     /locus_tag="VHE37_01030"
     CDS             complement(3523..5871)
                     /locus_tag="VHE37_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34137.1"
                     /translation="MKLNLVALLLLSAAGASRAQDSAAPPTPDAANADGAPAVVPTIP
                     VPEAATREPVVAEAQPENTTRLDDVVVTANKRRESSRTVAGAVTAIDSNRLKETGAST
                     MADYLSLSPGVNFDSGTPGYSVITIRGVSTDTIPGIAQTAVGTYYDDIPLTDPGAPMV
                     VPDIDAFDAQRIELLRGPQGALYGSASLGGALNYIPAPPNLAAPEYALQANGSLTHNS
                     SLASTGKLMFNLPLFANTTLGQYGLGVRGVGYYTHTPGYIDNIGLDREQSNSSHTGGG
                     RVVAGWAPTGNSVLRLTALYQRTEVDDAGYIDPSLGDLKKSTIEIEPSGNQLRLATLR
                     YELEQDYGSWAFIGGYQDKRMHLEYDGASALGVAAAKQQFPLRQFGGVTGYSGELRFV
                     SAPAERYNWLGGLSYATRTEDLWVTLDAPALQKLTGVLGALGSELNLGVLSTLAQDAT
                     LDQQHAIIKAPEAALFLDGTYYLTPTLKLAAGGRYYRNQVNSTVDSTGLLIAPTGTVQ
                     STTQGTVKAGGFNPKISLAWQAADRMLLYGLYSRGYRLGGINLVPASTVTGAKPKYGP
                     DEVKNYETGIKTSWLDGSLTADLSLFWIDWAAIPLVVVDRVGLFKYLDNAGNARSKGA
                     ELSLALRPWSFLTLRSALTYTDSRLLNDYDPKNNRPPAKAGDRLPGAPMFTTSNTATG
                     MWYLGSYTATASLIHRYEGESSTNLSFQDYKKGGDHLFDLRAGLSRGGLSCTLYGKNL
                     TDARAVNASNNYAKPGGDVLSLKFITPPRTVGVELGYTFSQE"
     gene            complement(5868..6587)
                     /locus_tag="VHE37_01035"
     CDS             complement(5868..6587)
                     /locus_tag="VHE37_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVT34138.1"
                     /translation="MASTAPARARRRPRPGRPRKLDYEDVIAAAFEVMNDEGFSTLSM
                     RSLAKKLGIAHGNLYTYVGHIEDVEAEVLKRLLERLPAPSTDPAGPAALRAELITFLH
                     ATRKVLFQHPHVMYPPIGSPAWVSFGKLTTQWISALARHESDARAVAVGFTALIAHVA
                     SHAQRERLVGPDHLARNLQAWRKTFQAHPALVEIERMARHGAEAALTQIFETLIDRLF
                     PALEQTARAPHARRARTGSKS"
     gene            6877..8436
                     /gene="msrAB"
                     /locus_tag="VHE37_01040"
     CDS             6877..8436
                     /gene="msrAB"
                     /locus_tag="VHE37_01040"
                     /inference="COORDINATES: protein motif:HMM:NF010625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HVT34139.1"
                     /translation="MSRSKWAWAWILGVCLTSTAACGKPGDAPKKAIDASAPAVDATL
                     TDLDGKKLGLSQYRGKKVYVKFWASWCPICLAGMGEVENLAAHAPADVQVLTIASPDY
                     LGEMEQQDFRAWAQARHFRVPILVDNGGHFAASLGIDRYPSAIWLSPGGKLLDVHTGQ
                     VSNDWIVAHFDEATGVKPRVPSRFPPNPNIGVDYSKAHLKEIWLAGGCFWGVEAYFTR
                     VYGVADAASGYANGSTPHPSYEDVCNGSGHAETVRVQYDPARVSLEKLLTLFFKIIDP
                     TSLNQQGNDRGRQYRTGIYYKDDADRAVAAAVMKSEQAQYSRPIVTELVPLAEFSLAE
                     DYHQDYLEKHPDGYCHTDFSSLTNTTMPPQPLHVDASRYHKPDDATLRKLLSPQQYEV
                     TQHADTEYAFSNQYWDNHAPGLYVDVVTGEPLFSSTDKYDSGCGWPSFTRPLVPEVVS
                     YRTDSTFGMTRTEVRSRVGNTHLGHVFDDGPADRGGKRYCINSASIRFIPVEELAKAG
                     YGDFAVLFGKR"
     gene            8771..>9117
                     /locus_tag="VHE37_01045"
     CDS             8771..>9117
                     /locus_tag="VHE37_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34140.1"
                     /translation="MLSPSLRAALLLLVLTLAVVLPGAPALASPGGAAPEPTVDPALF
                     RALQWRGIGPYRGGRALAVSGVPGDPYTFYAGFVAGGVWRSRDAGATWEPLTDHTPIV
                     SVGALALAPSDPR"
BASE COUNT         1446 a         2990 c         3156 g         1525 t
ORIGIN      
        1 ggaacgcgac ggcctgctca atcgcaccat cgagttcctg cccgacgacg aatcactgaa
       61 ggagcggcgc agccacggcc gcggcctgac gcggccggaa ctgtcggtgc tgctggccta
      121 ctcgaagatc tcgctgaagg acgcgatgct caagtcgcag gtgccggacg acccctactt
      181 cgagcgcgac ctgctggcca atttcccgcc gctgctggtg aagaagttcg gtgaggtgct
      241 ggtgcatcac cagctcaagc gcgagatcgt ggcgaccatg ctgaccaatg cggtggtcaa
      301 tcgcatgggc gtgtcgttca gccatcgcct ggccgaagac catggccagc cgcgcgcgca
      361 gatcatcaag gcgtacgcgg ccgcgcacca gatctacgat gccgaccgct actggacggc
      421 gatcgagtcg ctggacaaca aggtcaaggc cagcctgcag tacgcgctgt acgagcgcgc
      481 catcggcctg ctcaagcatg tcaccgggtg gatcgtgaac agcgacatgg cgcaggcgcc
      541 gatcggcgcc atcgtggcgc gctttgccgc ggccatcccg gccggcgaga agctgctgcc
      601 cgatgcgctg cccgcttcgt accgcgccga ctgggacaag gcggtggatt cgatggtgcg
      661 cgaaggagtg ccgcaggagc tggcggtgat gctggcgaac accaaggtgc tcggctcggg
      721 gcctgacgtg gtcgaactgg cggaagctgc caaggcgcca ctgaccgaga ccgcacaggt
      781 ctacttcgag atcggcgacc gcttttcggt gctgtggctg ctgtcggcca tcatcggctt
      841 gtcggtgcag agcaagtggc aggcgctggc gcgtgccaac ctgcgcgacg atgtctatcg
      901 cctgcagcgc cgcctcgcca gccaggcgct gcgcgcgccc ggcaagacgg cgcaggccaa
      961 ggtcgaagcg tggatggcgg ccaacgaaga agtagtcagc ttcgcgcgcc gtcgtctcaa
     1021 cgaactgcag accggcggcg cggccgactt catgtcgctg gcggtcgcgg tgcgggcgct
     1081 gcgcgatttg cgcacgcttt agccggcgcg atcaggcggg cagccggtca gccagtgcat
     1141 cgagcagccg cacgccgaac ccggtcgcgc ccttggggat gcccggcttc ggccgcgtga
     1201 tccacacggt gccggcaata tcgagatgcg cccagcgcgt gcctgcagcg aagcgttcca
     1261 ggaatttggc gccggcaatg gccgaaccgg cgctggtgcc gaaaaatccc ggcgcgccaa
     1321 ggttcttcac atcggcgatt tccgagtcga cgatgtaatc ctgtttcttg ctcagcggca
     1381 tgcgccacag gtcctcgccc tggctcgcgc cagcttctcc cagcgcggcg gcgagggctt
     1441 cgtcggtgct gaacagcccg gcatattcct cgtgcagcga ggccatgatc gaaccggtga
     1501 gggtggccac atccaccagc agccgcgggc ggaatttgtt gatgaggtga ctgatgccgt
     1561 cagccagcac gatgcgtccc tcggcgtcgg tgttgtcgac ctcgatggtc aggcccgaca
     1621 gcgaggtgat gacgtcaccc ggccggtagg agtcggcgtc gatcaggttt tcgcacagcg
     1681 gcagcgcggc gaccacgttg accggggcct tgcgctgcgc catcacgcgc agcgcgccca
     1741 ccaccgcggc ggcgccgccc atgtcgaact tcatcttgcc gatccccggc ggcatcttga
     1801 tattgaggcc gccggcatcg aaggtcaggc ccttgccgac caggccgaga tcgttttcct
     1861 tgctgctgcg gccctgccag cggcagatga ccatgcaggg tggatggacg ctgccgcgtc
     1921 caaccgccag caggccgcct gcgccgatgc gctccagcgc cttcaggtcg agcacttcca
     1981 cttcgatgcc gaacgacttc agcgatgcga tttcagcagc gaaagtttgc ggatggagca
     2041 ggttggccgg cgcgtccacc agcgcgcgtg cccagttgac cgcatcgcca agctcgcgcc
     2101 cgcgcatgcc ggccttccag tcggcagcag acagcgccag cgactcaacg ttgaaatggt
     2161 ctttcggagg ctgccggcga taagccacgc agcgatacga ggccagcgtg gcgccgatgg
     2221 caaatccgcg ggcggccgcg gcggcgtcga tcgccgccgg caccaggtcg ccgctgagcg
     2281 ccgcacgcgc aatcttcagc gagcgcattg catcgatcac cgctgcgccg gcgctttccc
     2341 agtacatcgg ctcctcggca gtggccggca tcttgccgca gccgaccacg agtagccgcg
     2401 ccttggccgc ggaaatttca atgcgctcgc cggtggtgcc acggaagctg gcgggcagcg
     2461 ccggcaattc ccgtcccgca gtgcgcagct ggcggtcttg gccgaccagc agtacctgta
     2521 cctccggcgc tgccttgcca gcggtgcaca gcggattggt cacctggtgc agcttgagtg
     2581 cgccattttt ttgttttgcc gcggaagatt tgttcatgct gggacgctca ggtcgtgggc
     2641 aggctggaaa gataatcgat catgcaggtc gcggttgccg ccgcgccgga gcgcgagacg
     2701 accagcgcgc cgtgcgtgtg aggcagcgtg atccagcggc tgcccgggcc gccgcgcccc
     2761 gcggtgatct cgtgctgggc ctgcatgcgg ccgagcagcg agcgcgcccg ctccggcgac
     2821 accagcaggc ggaacagcgc gttgcggact tcctcggtag tgccggcgct gatcaccagc
     2881 cgcggcaccg gcgagggctg ggcgcaaacg cgccggatgt cctgcagcag gtccagttcg
     2941 attcctgcgg cgcgcagcga agcggcattg ccgaaggcat tgcgccgcaa ctgcgccgtg
     3001 tccgcggcag gcaattcagg catgtgcggg gcgaagaacg gttcgggtat gcgcagggct
     3061 gccagcaggc gcagcacctt ccccaggccg cgcatgctgc ccagcgtggc gtcgatctgc
     3121 tcatcggtcc gctcggccgt ggggtcgatc tgcaccaccg cgcgcagacg ctgcggcatc
     3181 agcgcggcgt gcacgccgca gatcagcccg ccatacgact gcccggcgat gaccaccggt
     3241 gcgcggatgc cgagtgcgtc gagcagcgcg gccaggtgcc ggctcatgcc ttccgcggtg
     3301 agcgtttcat gcgccatgct gccgccgatg ccggcgcggt tgtagagcag cagctgcgtg
     3361 tgcggagcaa gtgcttcctg cagcagcgca aaatagtcgt acgacgcgcc ccagccattc
     3421 tccagcacga tggtcggcag tgttcccggc gtgccggcca ggcgatagcg cagctccgcg
     3481 ccgggaatct cggcgatgtc ttcgcgtgtc gtcatctgaa aatcactcct ggctgaaggt
     3541 atagcccagc tccacgccca cggtgcgcgg cggcgtgatg aacttgagcg acagcacgtc
     3601 gccgcccggc ttggcgtagt tgttggaggc gttgactgcg cgcgcatcgg tgaggttctt
     3661 gccgtacagc gtgcagctca agccgccgcg gctgaggccg gcgcgcaggt cgaacaagtg
     3721 gtcgccgccc ttcttgtagt cctggaacga caggttggtg gacgactcgc cctcgtagcg
     3781 gtggatcagg ctggcggtgg cggtgtagct gcccaggtac cacatgccgg tggcggtgtt
     3841 gctggtggtg aacatcggcg cgccgggcag gcggtcgccg gcttttgccg gcgggcggtt
     3901 gttcttgggg tcgtagtcgt tgagcaggcg cgaatccgtg taggtgagcg ccgagcgcag
     3961 cgtgagaaag gaccagggcc gcagcgccag gctgagttcc gcgcccttgc tgcgcgcgtt
     4021 gccggcgttg tccaggtatt tgaacaggcc gacgcggtcc accaccacca gcgggatcgc
     4081 ggcccagtcg atccagaaca gagagagatc cgcggtcagc gagccatcga gccagctggt
     4141 cttgatgccg gtctcgtagt tcttcacctc gtccgggccg tacttgggct tggcgccagt
     4201 gacggtggag gcgggcacca ggttgatgcc acccaggcgg tagccgcgcg agtacagccc
     4261 gtacagcagc atgcggtcgg cggcctgcca ggccagcgag attttcggat tgaagccgcc
     4321 ggccttcacc gtgccctgcg tggtcgactg caccgtgccc gtgggcgcga tcagcaggcc
     4381 ggtggaatcg accgtgctgt tgacctggtt gcggtagtag cggccgccgg cagcgagctt
     4441 gagtgtgggc gtgaggtaat acgtgccgtc gaggaacagc gcggcttccg gcgccttgat
     4501 gatggcgtgc tgctggtcta gcgtggcgtc ctgcgccagc gtggacagca cgcccaggtt
     4561 gagctcgctg cccagtgcgc cgagcacgcc cgtcagcttc tgcagcgccg gcgcatcgag
     4621 cgtgacccag aggtcttccg tacgcgtggc gtacgacagt ccgcccagcc agttgtagcg
     4681 ctcggccggc gcggagacga agcgcagctc gccgctgtag ccggtcacgc cgccgaactg
     4741 cctcagcggg aactgctgct tggcggcggc gacgcccagc gcgctggcgc cgtcgtactc
     4801 caggtgcatg cgcttgtcct ggtagccgcc gatgaaggcc cagctgccgt agtcctgttc
     4861 gagctcgtag cgcagcgtgg ccaggcgcag ctggttgccg ctgggctcga tctcgatggt
     4921 cgacttcttc aggtcgccca ggctgggatc gatgtagccg gcgtcgtcca cctcggtacg
     4981 ctggtacagc gcggtcaggc gcagcaccga gttgccggtc ggcgcccagc cggcgacgac
     5041 gcgcccgccg ccggtgtgcg atgaattgga ctgttcgcgg tccaggccga tgttgtcgat
     5101 gtagccgggc gtatgcgtgt agtaaccgac gccgcgcacg cccaggccgt actggcccag
     5161 cgtggtgttg gcgaacagcg gcaggttgaa catcagcttg ccggtgctcg ccaggctgct
     5221 gttgtgcgtg aggctgccat tggcctgcag cgcatattcc ggcgcggcca ggttcggcgg
     5281 cgcgggaatg tagttgagcg ctccgccgag cgaggccgag ccgtagagcg cgccctgcgg
     5341 gccgcgcagc aactcgatgc gctgcgcatc gaaggcgtcg atgtcgggca ccaccatggg
     5401 cgcgcccggg tcggtcagcg ggatgtcgtc gtagtaggtg ccgaccgcgg tctgcgcgat
     5461 gccgggtatg gtgtcggtgg agacgccgcg gatggtgatg accgagtaac ccggcgtgcc
     5521 ggagtcgaaa tttacgcccg gcgacagcga caggtaatcg gccatggtgc tggcgccggt
     5581 ctccttcagg cggttgctgt cgatggcggt gaccgcaccg gctaccgtgc gcgaactctc
     5641 gcgccgcttg ttcgcggtga ccaccacgtc gtccagccgc gtcgtgtttt ccggctgggc
     5701 ttcggccacg accggttcgc gcgtggccgc ttcgggcacg ggtatggtcg gcaccacggc
     5761 cggcgcgccg tcagcattgg cggcatccgg tgtcggcggc gcggcgctgt cctgggctct
     5821 actggcgccg gctgccgaca gcagaagcag ggcgaccaaa ttgagcttca tgatttcgat
     5881 ccggtccggg cccggcgtgc gtgcggcgcg cgcgcggttt gctcaagggc ggggaacagg
     5941 cggtcaatca gcgtttcgaa aatctgcgtc agcgcagcct cggcgccatg ccgcgccatg
     6001 cgctcgatct ccaccagcgc cgggtgcgcc tggaaggtct ttctccaggc ctgcaggttg
     6061 cgcgccaggt gatcgggccc caccagccgc tcgcgctggg cgtggctggc cacgtgggcg
     6121 atgagcgcgg tgaagcctac cgccaccgcc cgggcatcgc tctcgtggcg ggccagggcg
     6181 ctgatccact gggtggtgag cttgccgaac gacacccagg ccggcgagcc gatcggcgga
     6241 tacatgacgt gcggatgctg gaacagcacc ttgcgtgtgg catggagaaa ggtgatcagt
     6301 tcggcacgca gcgcggccgg gccggcggga tcggtgctgg gcgcgggcag gcgctccagc
     6361 aggcgcttga gcacctcggc ttccacgtcc tcgatatgcc cgacgtaggt gtagaggttg
     6421 ccgtgggcaa tgcccagctt cttggccagc gagcgcatcg acagcgtgct gaagccttca
     6481 tcgttcatca cttcgaacgc cgcggcgatg acgtcttcgt agtccagctt gcgcggccgg
     6541 cccggcctgg gccgtcgtcg cgcgcgggca ggggcggtgg atgccacggc gtgttctcct
     6601 cgtaataatt cgatacagct gtatttttac taatctagaa cagctgtatc gatattggca
     6661 agccagcaca gcaggacggc accgcgccgc cgcgcagcag agcgccatgg gtccgtcggc
     6721 gcggaacttt ggcggcgccg cgctgttctc tgtggagctg acgccgcgaa cgcgcggcat
     6781 caacagcggg acgcgcgggc ataggcaaaa gcgccgccgc cgccatactt caccgggtgc
     6841 ggccgccgct tccatggccg cgctaccgag gaaccgatga gcagatcaaa gtgggcatgg
     6901 gcgtggatac tgggcgtgtg cctgaccagc acggcggctt gcggcaagcc gggcgatgcg
     6961 ccgaaaaaag ccatcgacgc gtcggcgccg gcggtggatg ccacgctgac cgacctggac
     7021 ggcaagaagc tcggcctgtc gcagtaccgc ggcaagaagg tctacgtgaa gttctgggcc
     7081 agctggtgcc cgatctgcct ggccggcatg ggcgaagtcg agaaccttgc ggcgcacgcg
     7141 cccgcagacg tgcaggtgct gaccattgcc tcgccggatt atctcggcga aatggaacaa
     7201 caggatttcc gcgcctgggc gcaggcgcgc catttccgcg tgccgatcct ggtggacaac
     7261 ggcggccatt tcgcggcgtc gctcggcatt gaccgctacc cctccgccat ctggctgagc
     7321 cccggcggca agctgctgga cgtgcatacc ggccaggtgt cgaacgactg gatcgtcgcg
     7381 catttcgacg aggccaccgg cgtgaagccg cgcgtgccca gccgctttcc gcccaacccc
     7441 aacatcggcg tcgactacag caaggcgcac ctgaaggaaa tctggctcgc cggcggctgc
     7501 ttctggggcg tggaggcgta tttcacgcgc gtgtatggcg tggccgacgc cgcctcgggt
     7561 tatgccaacg gcagcacgcc gcatcccagc tacgaggacg tgtgcaacgg ctccggtcac
     7621 gcggaaaccg tgcgcgtgca gtacgatccc gcacgggtga gcctggaaaa actgttgacg
     7681 ctgttcttca agatcatcga tcccacctcg ctcaaccagc agggcaacga tcgcggccgg
     7741 caataccgca ccggcattta ctacaaggac gacgccgacc gcgcggtggc cgcagcagtg
     7801 atgaagtccg agcaggcgca gtattcacgg cccatcgtta ccgagctggt gccgctggcg
     7861 gaattttcac tcgccgagga ctatcaccag gactacctgg agaaacatcc cgacggctac
     7921 tgccacacgg atttctccag cctgaccaac accaccatgc cgccgcagcc gctgcatgtc
     7981 gacgcatcgc gctatcacaa gcccgacgac gcgacgctgc gcaagctgct gagcccgcag
     8041 cagtacgagg tcacgcagca cgccgacacc gagtacgcct tcagcaacca gtactgggac
     8101 aaccatgcgc cgggcctgta cgtggacgtg gtcaccggcg agccgctgtt cagctccacc
     8161 gacaagtacg actccggctg cggctggccc agcttcacgc gcccgctcgt gccggaggta
     8221 gtcagctacc gcaccgattc caccttcggc atgacgcgca ccgaggtgcg cagccgcgtg
     8281 ggcaacacgc acctgggcca cgtcttcgac gacgggcctg cggaccgcgg cggcaagcgc
     8341 tactgcatca acagcgcttc aatccgtttc atcccggtgg aagaactggc gaaggccggg
     8401 tatggagact ttgcggtgtt gttcggcaag cggtaaatgc ggcacccagc tcggtactga
     8461 tgcgatgagc gcccaacagc tacggtcggc caattccggt cggtagcgag ttgctgctct
     8521 agagtgaagc gccgggtatt cactagtctg aacagtgccg acttactcgt cgtaacccgg
     8581 ttcgtcgacg gcgccatccc ggcaactctg actgagagtc ggtgatgtgc caggcagttt
     8641 cgagcttcga agagctcgcc gcagccgtgc attcacccag tgtgccgagc gcggtggcca
     8701 atgcttcggc gtttaagccc ggcgagcaaa tcaggtaccc tcgacgcacc caccgggacg
     8761 agggatcccc atgttgtctc cgagcttgcg cgcggcgctg ctgttgctgg tcctgacact
     8821 ggccgtggtc ctgcccggcg caccggcgct ggcgagcccg gggggcgccg cgccggagcc
     8881 gaccgtcgac cccgcgctgt tccgcgcgct gcagtggcgc gggatcgggc cgtatcgcgg
     8941 cgggcgcgcg ctggcggtca gcggcgtgcc gggcgatccg tacaccttct acgcgggctt
     9001 cgtcgcgggc ggggtgtggc gcagccgcga tgccggcgcg acctgggagc cgctcaccga
     9061 ccacacgccg atcgtctcgg tcggcgcgct ggcgctggcg ccgtccgatc cgcgcat
//
LOCUS       DASZZC010000042         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_965618,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000042 DASZZC010000000
VERSION     DASZZC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965618"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VHE37_01050"
     CDS             <1..1104
                     /locus_tag="VHE37_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34141.1"
                     /translation="YFYQIHNTADAVFGQFTWSPEYFARRLHLTAGLRQSWDHRQAIK
                     DYAALEYLEGQAAGQPLAAIPLPATQGNDTFNDVHADKRYSDLSPSLNLQYDFTRSAT
                     GYFSVARAYKSGGFNLRDPQISGASGKASDGVNYGFGFVDGFKPERVLSYELGLKSEW
                     FGRRLRVNGDVFDSEYRDMQTNFLISGTISDTKSRNAGRARMRGVELDTTWAAAQGLV
                     LAFDYAYLNTRTLEVIDLNGKNLAGLYPFVSAPPQSYVGSIDWTFLEGGWGSLRAYAS
                     YNWTGHREGLVVTEPRRGKTATQGYGLFNARIVAGGVHVGAHGSLDLALWGKNLADVE
                     YPSTAIDNLPQADRAVIWGEPRSFGLDAVYRWD"
     gene            1185..>2305
                     /locus_tag="VHE37_01055"
     CDS             1185..>2305
                     /locus_tag="VHE37_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34142.1"
                     /translation="MNSTRRSIAASAALCVLLAACNAGTDVTGAGTAVATKADLKDVF
                     EGPVPRAACGAGSLPETDIQGRVSIDDRNSGRSLLGYSCNLQLLGQYQGQGTTWVSAS
                     YRHCAYHGQTVTSIASSTPGVNVVDVSDPAHPVLSERLTSPAFLGNTWETLKVNEARG
                     LLGGVMVGPILGAAFFDVYDISGDCAHPVLLNSVGESQLTLPANTLGHEGNWSPDGKT
                     YWSSAVVGGVITAIDVSDPAHPSIVYTGSTGLANHGFEISADGNRLYIAEAGAQGVLP
                     NGLHIYDISEIQQRKPLPQMRLLGSVTWTDGQLGQHAVPVSWNGRPYLIFVDEQASGA
                     ARIIDIADETAPRVVSKLKLEIHMPENAALRSTDTQGNGV"
BASE COUNT          403 a          787 c          761 g          354 t
ORIGIN      
        1 tatttctacc agatccacaa caccgcggac gcggtgttcg gccagttcac ctggtcgccg
       61 gaatacttcg cgcgccgcct gcatctcacc gccggcctgc gccagtcgtg ggatcaccgc
      121 caggcgatca aggactacgc cgcgctggaa tatctcgaag gccaggcagc aggccagccg
      181 ctggccgcca tcccgctgcc ggcgacgcag ggcaacgaca ccttcaacga cgtgcatgcc
      241 gacaagcgct acagcgatct ctcgccgtcg ctgaacctgc agtacgactt cacccgcagc
      301 gcgaccggat atttctcggt ggcgcgcgcg tacaagagcg gcggcttcaa cctgcgcgat
      361 ccgcagatca gcggcgcgtc gggcaaggcg tccgacggcg tgaactacgg cttcggtttc
      421 gtcgacggct tcaagcccga gcgcgtgctg tcgtacgaac tgggcctgaa gagcgagtgg
      481 ttcggccgcc gcctgcgcgt caacggcgac gtgttcgatt ccgagtaccg cgacatgcag
      541 accaacttcc tcatctccgg caccatctcc gacaccaaga gccgcaacgc cgggcgcgcg
      601 cgcatgcgcg gcgtggagct cgataccacc tgggccgccg cgcagggact ggtgctggcg
      661 ttcgactacg cctacctgaa cacgcgcacg ctcgaggtga tcgacctcaa cggcaaaaac
      721 ctggccggtc tctacccgtt cgtgtcggcg ccgccgcagt cgtacgtggg cagcatcgac
      781 tggacgttcc ttgaaggcgg ctggggcagc ctgcgcgcgt acgccagcta caactggacc
      841 gggcatcgcg aaggtctggt ggtgaccgag ccgcggcgcg gcaagacggc gacacagggt
      901 tatggactgt tcaacgcgcg catcgtcgcg ggcggcgtgc acgtgggcgc gcatggttcg
      961 ctggacctcg cgctgtgggg caagaacctg gccgacgtgg aatatccctc gaccgccatc
     1021 gacaacctgc cgcaggccga ccgcgcggtg atctggggcg agccgcgcag cttcggcctc
     1081 gatgccgtgt atcgctggga ctgagccctg tccttctttt tgtcgattta caggaccgcg
     1141 gtgaccgcag acactgcatg cattccatga aggcaggaga gagcatgaat tccacgcggc
     1201 gcagcatcgc ggcaagcgct gcgctctgcg tgctgctggc agcctgcaac gcaggcactg
     1261 atgtcaccgg cgcaggaacg gcggtcgcaa ccaaggccga tcttaaggac gtgttcgaag
     1321 gcccggtgcc gcgcgcggca tgcggcgccg gctcgctgcc ggagactgat atccagggcc
     1381 gcgtgtcgat cgatgaccgc aacagcgggc gctcgctgct cggctattcc tgcaacctgc
     1441 agctgctcgg ccagtaccag ggccagggta cgacctgggt cagcgcctcg taccggcact
     1501 gcgcgtatca cggacagacc gtgacctcca tcgcgtcctc cacgcccggc gtcaacgtgg
     1561 tggatgtgag cgatccggcg cacccggtgc tgagcgaacg cctgaccagc cccgccttcc
     1621 tcggcaatac ctgggagacg ctcaaggtca acgaggcgcg cggactgctc ggcggcgtca
     1681 tggtggggcc gatcctgggc gcggcgttct tcgacgttta cgacatctcc ggcgactgcg
     1741 cgcacccggt gctgctcaac agcgtgggcg aatcgcagct gacgctgccg gccaacacgc
     1801 tgggccacga gggcaactgg tcgcccgacg gcaagaccta ctggtcgagc gcggtggtgg
     1861 gcggcgtgat caccgccatc gacgtgagcg atccggcgca cccgtccatc gtctacaccg
     1921 gcagcaccgg gctggccaac cacggcttcg agatctccgc cgacggcaac cgcctgtaca
     1981 tcgccgaggc cggcgcgcag ggggtgctgc ccaacggcct gcacatctac gacatcagcg
     2041 agatccagca gcgcaagccg ctgccgcaga tgcgcctgct gggcagcgtg acctggaccg
     2101 acggccagct cggccagcat gcggtgccgg tgagctggaa cggccggcct tacctgatct
     2161 tcgtcgacga gcaggcatcg ggtgcggcgc gcatcatcga catcgccgac gagacggcgc
     2221 cgcgcgtggt gagcaagctc aagctggaaa tccacatgcc ggagaacgcc gcgctgcgca
     2281 gcaccgatac gcagggcaac ggagt
//
LOCUS       DASZZC010000043         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1096266,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000043 DASZZC010000000
VERSION     DASZZC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096266"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VHE37_01060"
     CDS             complement(<1..259)
                     /locus_tag="VHE37_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34143.1"
                     /translation="MRWNFLPRDVRGQRTLVPLIVACALFMENLDSTIISTALPAIAR
                     SFNEDPLHLSLAITSYLVSLAIFIPLSGWMADRYGARSVLCN"
     gene            complement(261..749)
                     /locus_tag="VHE37_01065"
     CDS             complement(261..749)
                     /locus_tag="VHE37_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34144.1"
                     /translation="MIRSLCTASLALALAACAATPAAYQPLRGGIGYQEQRLDADRYR
                     VSFAGDYRMPRQTVETYLLYRSAELTLAAGRDYFVVESRDLQQQQEHSATGFAFGVGA
                     FGSHSVTGVGVDPNEEAVATDFTASATISLHRGSKPLNDANAYDAHQVQANLSAQIVR
                     PH"
     gene            complement(806..1537)
                     /locus_tag="VHE37_01070"
     CDS             complement(806..1537)
                     /locus_tag="VHE37_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HVT34145.1"
                     /translation="MLSNRYAVIVLALCGTLLAGCHKDDMRAVNAIADANPMDVTVDG
                     TSSTISSVAYGSYGTASGIKDDTYTVELSTGNDSVDVNIVVTPVTVAQKQLSTIYAIG
                     RIADGSQAALVVQQADTDVADAQTLLQLVDAQTQLIGALDIYFTGLSDSLSGVAPLAT
                     LTYPATPAATTIRADSYRIRITPSGDQGTILFDSGASTGVSFASADSVQLALLDNTDT
                     TIDSKLRLLLLTRGGSLTTVQHVSP"
     gene            1783..2631
                     /locus_tag="VHE37_01075"
     CDS             1783..2631
                     /locus_tag="VHE37_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT34146.1"
                     /translation="MNAGLCELRLPIDGIDTPLLTGGDGHSSEAVLFVHGNPGSGRDW
                     EALAAGAAEFMRVLAPDLPGYGRAAKPDAFPCTVESYAAHLDALLRAQQVQRVHLVLH
                     DLGGAWGLAWAALHPHQLASLTLINTGVLLDYRWHIAARIWQTPLLGELFQLLATRGA
                     FHRSLQLRGARPLPRAFIDRMYDDYDAATRRAVLRTYRSMRQPQEQIEPWLPALRALQ
                     VPTQVIWGARDPYIPANYAQRQREIFAQAQVTLLEDSGHWPFADHPERVAQLLLPFLR
                     SVRSVR"
     gene            complement(2628..>2928)
                     /locus_tag="VHE37_01080"
     CDS             complement(2628..>2928)
                     /locus_tag="VHE37_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT34147.1"
                     /translation="EALIATPVDLTEQLRQKARMQLDRLCQRVGVPLERTRVLSGPVP
                     LQILDTATELGADLIVVGHAPRRGWLAALFSHTDQSVVSRAPCDVLALKLPEEKT"
BASE COUNT          434 a          981 c         1023 g          490 t
ORIGIN      
        1 cgttgcacag cacgctgcgc gcgccgtagc ggtcggccat ccagccggac agcgggatga
       61 agatggccag gctcaccagg tacgaggtga tggccagcga caggtgcagc gggtcttcgt
      121 tgaacgagcg cgcgatggcc ggcagcgcgg tgctgatgat ggtggagtcc aggttctcca
      181 tgaacagcgc gcaggccacg atcagcggca cgagcgtgcg ctggccgcgc acatcgcgcg
      241 gcaggaaatt ccagcgcacg ctagtgcggc cgcacgatct gcgccgacag gttggcctgc
      301 acctggtgcg cgtcgtaggc gttggcgtcg ttcagcggct tgctgccgcg atgcagcgag
      361 atggtggcgc tggcggtgaa atcggtggcc accgcttcct cgttcggatc gaccccgacg
      421 ccggtcacgc tgtgcgaacc gaacgcgccg acgccgaagg caaaacccgt ggcgctgtgc
      481 tcctgctgct gctgcaggtc gcgcgactcg accacgaagt agtcgcggcc ggccgccagc
      541 gtgagttcag cggagcggta cagcaggtag gtctccaccg tctggcgcgg catgcggtag
      601 tcgccggcga agctgacgcg gtagcgatcg gcatccagcc gctgttcctg gtagccgatg
      661 ccgccgcgca gcggctggta ggccgccggc gtggcggcgc aggcggcgag cgccagggcc
      721 aggcttgccg tgcacaggct gcggatcatc gatgcacctc cacgctccat tggactgcag
      781 cggccgcgca gcgttcgcgc gcgcgtcagg ggctcacgtg ctgcacggtg gtcagcgagc
      841 cgccgcgcgt gagcagcagc aggcgcagct tggaatcgat cgtggtgtcg gtgttgtcca
      901 gcagcgccag ctgcacgctg tcggccgagg cgaacgacac gccggtgctg gcgccgctgt
      961 cgaacaggat ggtgccctgg tcgccgctgg gcgtgatgcg gatgcggtag ctgtcggcgc
     1021 ggatggtggt ggccgccggc gttgccgggt aggtcagcgt ggccagcggc gccacgcccg
     1081 acagcgagtc ggacagcccg gtgaaataaa tgtcgagcgc accgatcagc tgggtctgcg
     1141 catcgaccag ctgcagcagc gtctgcgcat cggccacatc ggtgtcggcc tgctgcacca
     1201 ccagcgccgc ctgcgagccg tcggcgatgc gcccgatggc gtagatggtg gacagctgct
     1261 tctgcgcgac ggtcaccggg gtgaccacga tgttcacgtc gacgctgtcg ttgccggtgc
     1321 tcagttccac cgtgtaggtg tcgtccttga tcccgctggc ggtgccgtag ctgccgtagg
     1381 cgacgctgct gatggtgctc gaggtgccgt cgacggtcac gtccatcggg ttggcatcgg
     1441 cgatggcatt gaccgcgcgc atgtcgtcct tgtggcagcc ggcgagcagc gtgccgcaca
     1501 gcgcgaggac gatgaccgcg tagcgattcg acaacatgtg gaaatttcct gtgatcggcg
     1561 cggcgcagta tccgcgatgc ggcgcgcagc gtgtccgcca tagacccgcg ccgagtgtaa
     1621 aggtgcgttg agccggcgca aaaaagcagc caccgcacct gcttttatca tgttattaat
     1681 tattgattat ttttcaatta atataatttt ttataacatg ctccatgcca ttgcggtggg
     1741 gcccgttgca cgcccctggc gtggctgcgt catcatgccc gcatgaatgc cggactctgc
     1801 gagctgcgcc tgcccatcga cggcatcgac acgccgctgc tcaccggcgg cgacgggcac
     1861 agcagcgaag ccgtgctgtt cgtgcacggc aatcccggct cggggcgtga ctgggaagcg
     1921 ctggccgccg gcgctgcgga gttcatgcgc gtgctggcgc ccgacctgcc ggggtacggc
     1981 cgcgccgcca agcccgatgc tttcccctgc accgtggaaa gctacgccgc gcacctcgat
     2041 gccctcctgc gcgcgcagca ggtgcagcgc gtgcacctgg tgctgcacga cctcggtggc
     2101 gcctggggcc tggcctgggc cgcgctgcat ccgcatcagc tggccagcct cacgctgatc
     2161 aacaccggcg tgctgctcga ttaccgttgg catatcgcgg cacgcatctg gcagacgccg
     2221 ctgctgggtg aactgttcca gctcctggcc acgcgcggcg cattccaccg ctcgctgcag
     2281 ctgcgcggtg cgcgcccgct gccgcgcgcc ttcatcgacc gcatgtatga cgactacgac
     2341 gccgccacgc gacgcgcggt gctgcgcacc tatcgctcca tgcgccaacc gcaggagcag
     2401 atcgagccct ggctgccggc gctgcgcgca ctgcaggtgc ccacgcaggt gatctggggc
     2461 gcgcgcgatc cgtacatccc cgcgaactac gcgcagcgcc agcgcgagat ctttgcgcag
     2521 gcacaggtga cgctgctcga agacagcggc cactggccgt ttgccgacca cccggagcgc
     2581 gtggcgcagc tgctgctgcc gttcctgcgc tcggtgcgca gcgtgcgcta ggttttttcc
     2641 tccggcagct tcagcgccag cacgtcgcac ggcgcgcgcg acaccacgct ctggtcggtg
     2701 tggctgaaca gcgccgccag ccagccgcgg cgcggcgcgt ggccgaccac gatcaggtcc
     2761 gcgcccagct cggtggcggt gtcgaggatc tgcagcggca ccgggccgga cagcacgcgc
     2821 gtgcgctcca gcggcacgcc gacccgttgg cacaggcggt ccagctgcat gcgcgccttc
     2881 tgcctcagtt gctccgtgag atcgaccggc gtggcgatca gcgcttcg
//
LOCUS       DASZZC010000044        15326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_4258,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000044 DASZZC010000000
VERSION     DASZZC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15326
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4258"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VHE37_01085"
     CDS             complement(<1..287)
                     /locus_tag="VHE37_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVT34148.1"
                     /translation="MKPAAAAALAWAVRLLSALSLLFTSGPVLPQTALEQQVRAAFLY
                     NFARFTSWPVERVADSAPIVFCTLEHEPLGAALASTLAGKSIDQHLLQVRVA"
     gene            complement(284..2317)
                     /locus_tag="VHE37_01090"
     CDS             complement(284..2317)
                     /locus_tag="VHE37_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT34149.1"
                     /translation="MTRTRQVRRAWCDAARWLLCTLAVVMGPCAHAGVTPLEVASLPT
                     DLTQLSLDDLLKIQVVSVSKKAEPVGEAAAAVFVLSGDDIARSGVHRIADALRMVPGL
                     QVAQPNANNYDISARGFANTGDKLEVLVDGRSVYSPLTSSVFWDVLDTYLPDIERIEV
                     IRGPGATLWGANAVNGVINIVTRSARDTVGAAADVYGGNELRYGGSARLGLRTGDNGA
                     LRLYAQGHSLDGAARADNSALADGMNEARAGFRGDWQLGSGSLMATGGGYEATENFNT
                     VSTSGTLGAPVEADRSGGHLLARYECCAQVDGGWSLQASYDGYRVNEPTAYAEQRRTG
                     TLDFQQQWLLGTRHTLIYGAAMKFSRDSTAGPPMAIIFDPAARTLSTYSTFVQDQVSL
                     LDGAGTLTLGSKFEHNSLSGFEVQPGVRFGLKVTPALFSWASISRAVRTPNRIDDDVA
                     IYCPPPAGFPGVCGPGVFRIGNPDEDSEKVVAYEWGARLRTGATLSWDLASFFNDYTH
                     LRSTEPGAALPHFANELDGHGYGAELAVVWKPLQQLELRPWYSYQALHIHQRPGGADM
                     TAPANDEGSAPHHQAGLRVLYEPAPRWNVDAYLRYVSRLAQTSTPSASSGPTSVPAYT
                     ELSLRVARQLGSGLELSVAGDNLLDAQHAEFGASASRGELQRSLLLELRWAMP"
     gene            complement(2468..3010)
                     /locus_tag="VHE37_01095"
     CDS             complement(2468..3010)
                     /locus_tag="VHE37_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVT34150.1"
                     /translation="MAFNRHIARLFGGLALGLALVSPAQGADDLEQKVRAAFLYNFAK
                     FVSWPGYKTPQASDPLLFCVLDSDPFAGPLEQNLQGKTVDTHPVQVRRVQRVDDMRSC
                     HVAYLSIAESARLAVALEALAGNGVLTVFQGDAAQHAGIVRFFLEDQRMRFEINTAAA
                     ERERLEISSRLLGVARVVRE"
     gene            complement(3168..3716)
                     /locus_tag="VHE37_01100"
     CDS             complement(3168..3716)
                     /locus_tag="VHE37_01100"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HVT34151.1"
                     /translation="MSTPPPHAETPAAKPVSASALREQAYFVFPNDLNANDTVFGGLI
                     MAQMDRYCAVVADRHAQGVCVTVGVDAVHFIAPARRGDVLIFNACVNRSWNSSMEVGC
                     KVDAEKVGGGERRHILSAYLTFIAVDAQGKPRPVPAVLPETAIEKLRYDEAQLRREQR
                     LRHAAELEALRAAAAALKPAPG"
     gene            complement(3824..4345)
                     /locus_tag="VHE37_01105"
     CDS             complement(3824..4345)
                     /locus_tag="VHE37_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34152.1"
                     /translation="MSVVVHSLSGASTLAIFGPLMAWSVYGRVKRNVGPQPLKERTLW
                     IRIGILLAILIGFTRIDDLLLLSTAAGLALGAALAQFGLRLTRYEFTPGGNFYIPNAW
                     VGMAVSLLFVARIGYRMFVIYGTDGGMAAAMTRTPLTMGLLALVIGYYLAYYGGVLRQ
                     AKRHPLLHQAQQT"
     gene            complement(4378..5841)
                     /locus_tag="VHE37_01110"
     CDS             complement(4378..5841)
                     /locus_tag="VHE37_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HVT34153.1"
                     /translation="MGKVTGFMEFDRQKESYLPVDARLKNYKEFVLVLEDAKAKIQGA
                     RCMNCGTPFCTTGCPVNNIIPDWNDLVYTGNYRAALDTLHSTNNFPEFTGRVCPAPCE
                     AACTLNVNRDPVGIKSIELFIVEKGYENGWIVPQPAAVKTGRKVAIVGSGPSGLAAAQ
                     QLARAGHDVTVFEKNDRVGGLLRYGIPGFKLDKSYVDRRLEQMKAEGVTFRTSTYVGT
                     KPLQNITNWATTTVSPDQLMQDFDAVLLTGGAEQGRDLPVPGRELKGIHFAMEFLPLE
                     NRVDAGDQVKDQIRADGKHVVVIGGGDTGSDCVGTSNRQGAAGVTQFEVLPRPPDQEN
                     KPLVWPYWPIKLRTSSSHEEGCERDWSVGTKRFEGKDGKVEKLIAARLEWKDGKMSEV
                     PNSEFEVKADLVLLAMGFVAPAAAVLEGFGVEKDARGNAKATTDGEGCYKTSVNKVFA
                     AGDMRRGQSLVVWAIREGRQAARAVDEFLMGASDLPR"
     gene            complement(5872..10584)
                     /locus_tag="VHE37_01115"
     CDS             complement(5872..10584)
                     /locus_tag="VHE37_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HVT34154.1"
                     /translation="MQHRQLAETHGLYRSEHEHDACGIGFIAHIKGGKSHSIIQQGLQ
                     ILENLEHRGAVGADKLMGDGAGILIQIPDQLYREEMGRQGVELPPLGEYGVGMVFLPK
                     ENASRLACEQEIERAVREEGQVLLGWRDVPVDRDMPMSATVRAKEPVIRQIFIGRGDD
                     IMVTDALERKLYVIRKSSGHAIQALNLIHGKEFFVPSMSARTVVYKGLLLADQVGVYY
                     QDLRDERCISALALVHQRFSTNTFPEWPLAHPYRLIAHNGEINTVKGNFNWMRAREGV
                     MKSPELGDDLKKLFPLIYEGQSDTACFDNALELLVMAGYPIAQAMMMMVPEAWENHTL
                     MEPQRRAFYEYYAAMMEPWDGPAALAFTDGRYIGGTLDRNGLRPARFIITDDDLVVMA
                     SEAGVLPIPDARITKKWRLQPGKMFLIDLEEGRIIEDDELKDTYARAEPYKAEINRIR
                     LKLDELKLPKNAKAVEPVEAPKASLLDRQQAFGYTQEDLRILLTPMAVDGEEAVGSMG
                     NDSPPAVMSNKLKPLYSYFKQLFAQVTNPPIDPIREAMVMSLVSFIGPRPNLLDINNL
                     NPQMRLEVSQPVLDPADIARLRNISAYTRGKFKSKELHICYPVAWGKEGIEARLASLC
                     AKAVDAVKSGHNVLILTDRTMDAENVAIPALLATSAIHQHLVSKGLRTSTGLVVETGS
                     AREVHHFALLGGYGAEAIHPYLAMETLADMAKGLPGELSPEKAIYNFKKAIAKGLMKV
                     MSKMGISTYMSYCGAQIFEAIGLNRSLVDKYFRGTASNIEGIGVFEVAEEAVRLHKLA
                     FGKDPVLANMLDAGGEYAFRVRGEEHMWTPDAIAKLQHSTRANSYGTYKEYAQIINDQ
                     SKRHMTLRGLFEFKIDPAKAIAVDEVEPAKEIVKRFATGAMSLGSISTEAHTSLAVAM
                     NRIGGKSNTGEGGEDPLRYRNELKGISIKAGETMASVLGSDRVEATIPLQEGDSLRSK
                     IKQVAAGRFGVTAEYLNSADQIQIKMAQGAKPGEGGQLPGKKVSEYIAQLRFSVPGVG
                     LISPPPHHDIYSIEDLAQLIHDLKNANPRADISVKLVAEVGVGTVAAGVCKAKADHVV
                     IAGHDGGTGASPLSSVKHAGSMWELGLAETQQTLVLNRLRSRIKVQADGQMKTGRDVV
                     IAGLLGADEMGFATAPLVTQGCIMLRKCHLNTCSVGVATQDPVLRRKFAGKPEYVVNF
                     FFFVAEEVRQIMAQLGIRKFNDLIGRSDLLDQAKAIAHWKARGLDFSRVFYQPATDEP
                     RYQVLTQEHGLEKALDHKLIAQARPALEKGERVSFISPVGNYNRTVGTMLSGEVAKRY
                     GHEGLPDDTIHIQLQGSAGQSAGAFLAHGVTIDLVGEGNDYVGKGLSGGRIIVRPNTD
                     FRGWAVDNIIIGNTVLYGAISGEAYFNGVAGERFAVRNSGAVTVVEGTGDHGCEYMTG
                     GTVVVLGATGRNFAAGMSGGIAYVYDPDGSFEKKCNLAMVALEPLATSAEQEKTDKAI
                     WHRIERGGRPQTDEAILKSLIERHFKLTGSTRARNLLDDWMSSRRKFVKVFPHEYKRA
                     LAEMHATPAPAKKEPLAA"
     gene            complement(10732..11433)
                     /locus_tag="VHE37_01120"
     CDS             complement(10732..11433)
                     /locus_tag="VHE37_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT34155.1"
                     /translation="MARLPRLVVPHHPHHVIQRGNNRQAIFLDDDDRLQLLELLGAHA
                     RRDKVALHGYVLMDNHFHLLATPATQSGLSAMMQGVGRRYVRYFNQRYQRTGTLWEGR
                     YRATPVQTERYLLACMAYIDLNPVRAGMVARAADYRWSSYGHYAGLRSDPLITTHPLY
                     WQLGNTPFAREAGYVELVGQGVAAQTQALLTDTALKGWVLGDVDFVAGLQKQTPRRMT
                     RARAGRPFSKTKAVK"
     gene            complement(11439..12584)
                     /locus_tag="VHE37_01125"
     CDS             complement(11439..12584)
                     /locus_tag="VHE37_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HVT34156.1"
                     /translation="MTFQLAPCAASDEHSRGRRHAESAPSYRSQYQRDRDRIVHSAAF
                     RRLEYKTQVFVNHEGDLFRTRLTHSIEVAQIARTIARTLSLNEDLAEAISLAHDLGHT
                     PFGHAGQDALNAAMKPYGGFEHNLQSLRVVDELEDKYAEFRGLNLTFETREGILKHCS
                     LKHARKLGDVGQRFIRRQQPGLEAQLANIADEIAYNNHDIDDGLRARLLSIEQLREVP
                     LFRRHHDEVIRRYGEVTQKQQRHETIRRIINTLVSDLTQNSRAALARARPRHIDEVRA
                     RAKPLISYSEKIAQENQVLKRFLRENLYKHHQVYRMTVKAQRVVCDLFAALIDDARLM
                     PPDFHAQSQAAADRDARARVVADYIAGMTDRYALDEYERLHDPRRLR"
     gene            complement(12714..13802)
                     /gene="aroB"
                     /locus_tag="VHE37_01130"
     CDS             complement(12714..13802)
                     /gene="aroB"
                     /locus_tag="VHE37_01130"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HVT34157.1"
                     /translation="METLTVQLGTRSYPIRIGNGLLADAANFAETHGRPLKIVSDAQV
                     AAHYLPALLRTLKLDAADALVLPAGETQKTWNNAEHVLDWLLAARLPRDGMLAALGGG
                     VIGDLAGFCAAIYQRGIDFVQIPTTLLAQVDSSVGGKTGVNHARGKNLIGAFHQPRAV
                     VADLATLTTLPRRELSAGLAEVIKYGMLADALFFDWLDQNLDALLALEPRALAQAVRR
                     CCELKAQIVGVDERESISGGPRALLNLGHTFGHAIETYTGYSSWLHGEAVGAGLCMAA
                     DLSARLGWISDSDAQRSRHLVARAGLPVRPPPGMRAQHFMELMKLDKKVAGGKLRLVL
                     LRRLGDAMLSADYDATALAATLAAFSEA"
     gene            complement(13857..14390)
                     /gene="aroK"
                     /locus_tag="VHE37_01135"
     CDS             complement(13857..14390)
                     /gene="aroK"
                     /locus_tag="VHE37_01135"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HVT34158.1"
                     /translation="MGSKSNIFLIGPMGAGKTTVGRRLAEVRGMEFLDSDHEIEARTG
                     VDIAYIFEKEGEEGFRKREKQVLAEITQRSNIVLATGGGSVLDPENRQMLSARGLVVY
                     LHASINQQMQRTERSDNRPLLSRADDRREVLEELFARREALYREIADLVLATDGRNAR
                     LLVRDIEDHLARPATAF"
     gene            complement(14412..>15326)
                     /gene="pilQ"
                     /locus_tag="VHE37_01140"
     CDS             complement(14412..>15326)
                     /gene="pilQ"
                     /locus_tag="VHE37_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HVT34159.1"
                     /translation="RGHVTVDERTNQLIVLETREKLAEIRALVAQLDVPVRQVLIESR
                     IVIAHDDYRKELGARFGVTSVGTANGGNNLLATTGSSSGTDTMINSALSTGGTAVTLP
                     SQANRFNVNLPTASANPGSFALAILGANYLVDMELSALQIEGRGEVLSTPRVITANGK
                     EAVVEQGTEIPYLEAASSGAATVAFRKAVLSLTVNPQITPDSRILMDLTVTDDTQGAN
                     VAVGLGTAPSIDTRRLSTQVLVRTGETVVLGGVFDSTQEHDTSKVPFLGDLPLLGNFF
                     KNSDNNNTKRELLIFVTPKILQEGLKVH"
BASE COUNT         2460 a         4981 c         5079 g         2806 t
ORIGIN      
        1 gccacgcgca cctgcagcag gtgctggtcg atgctcttgc cggccagggt actggccagc
       61 gccgcgccca gcggctcgtg ctcgagcgtg cagaacacga tgggagcgga gtcggccacg
      121 cgctccaccg gccaggaggt gaagcgcgcg aagttgtaca ggaaggccgc cctcacctgc
      181 tgctcgagcg ccgtttgcgg cagcaccggg cccgaggtga acaacaggga caaggcggac
      241 agcaggcgca ccgcccacgc aagcgcagca gcggcagcgg gtttcacggc atggcccagc
      301 gcaactccag cagcaggctg cgctgcagtt cgccgcggct ggcgctggca ccgaattccg
      361 cgtgctgcgc gtcgagcagg ttgtcgcccg ccacgctcag ttccaggccg ctgcccagct
      421 ggcgcgccac gcgcaggctc agctcggtat aggccggcac cgaggtcggc ccgctggatg
      481 cgctcggcgt actggtctgc gccagccggc tgacgtagcg caggtaggca tcgacattcc
      541 agcgcggcgc cggctcatac agcacgcgca ggccggcctg gtgatgcggc gcgctgcctt
      601 cgtcgttggc cggcgcggtc atgtcggcgc cgccgggccg ctggtggatg tgcagcgcct
      661 ggtacgagta ccacggccgc agttccagtt gctgcagcgg cttccacacc acggccagtt
      721 cggcgccgta gccatggcca tcgagctcgt tggcgaaatg cggcagcgct gcgcccggct
      781 cggtgctgcg caggtgggtg tagtcgttga agaagctggc caggtcccac gacagcgtgg
      841 cgccggtgcg caggcgggcg ccccattcgt aagccaccac cttttccgag tcctcgtcgg
      901 ggttgccgat gcggaacacg ccgggaccgc acacgcccgg gaaaccggcg ggcggagggc
      961 aatagatggc gacgtcgtcg tcgatgcggt tgggcgtgcg caccgcgcgc gagatggagg
     1021 cccagctgaa cagcgccggc gtcaccttga ggccgaagcg cacgccgggc tgcacttcga
     1081 aaccggacag gctgttgtgc tcgaacttgc tgccgagggt gagcgtgccg gccccgtcga
     1141 gcaggctgac ctggtcctgc acgaaggtgc tgtaggtgga cagcgtgcgc gcggccggat
     1201 cgaagatgat ggccatcggc gggccggcgg tgctgtcgcg cgagaatttc atcgccgcgc
     1261 cgtagatcag cgtgtgccgc gtgcccagca gccattgctg ctggaagtcc agcgtgccgg
     1321 tgcggcgctg ctcggcgtag gccgtcggtt cgttcacccg gtagccgtca tacgacgcct
     1381 gcagcgacca gccgccatcg acctgcgcgc agcattcgta gcgggccagc agatggccgc
     1441 cgctacgatc ggcttcgacc ggcgctccaa gggtgccgct ggtgctgacc gtgttgaaat
     1501 tctcggtggc ctcataaccg ccgccggtag ccatcaggct gccgctgccc agctgccaat
     1561 cgccacggaa gccggcgcgg gcctcgttca tgccgtcggc gagcgctgag ttgtcggccc
     1621 gcgcggcgcc gtcgagcgaa tggccctggg catacaggcg caacgcgccg ttatcgccgg
     1681 tgcgtaagcc cagccgcgcg gagccgccat aacgcagttc gttgccgccg tagacatcgg
     1741 ccgcggcgcc aacggtgtcg cgcgcgctgc gggtgacgat gttgatcacg ccgttgaccg
     1801 cgttggcgcc ccacagggtg gcgcccgggc cgcggatcac ctcgatgcgc tcgatgtcgg
     1861 gcaagtaggt gtcgagcacg tcccagaaca ccgaggaggt cagcgggctg tacacgctgc
     1921 ggccgtcgac cagcacctcg agcttgtcgc cggtgttggc gaagccgcgc gcgctgatgt
     1981 cgtagttgtt ggcattgggt tgagccacct gcaggccggg caccatgcgc agcgcatcgg
     2041 cgatgcggtg cacgccagag cgcgcgatgt cgtcgcccga cagcacgaac accgccgccg
     2101 ccgcttcgcc gaccggctcg gccttcttcg acacggagac cacctggatc ttgagcaggt
     2161 catcgagcga cagctgggtc aggtcggtcg gcagcgaggc cacttccagc ggcgttaccc
     2221 cggcatgcgc gcacggcccc atcacgacgg ccaaggtgca cagcagccag cgtgcagcat
     2281 cgcaccaggc gcgccggacc tgcctcgtgc gcgtcatgtg ccagtcctcc cgtgcgcgcc
     2341 acccgtgacc cattgtgggc tgatcttgcc cactgtcctg tagagggaaa gcctcattcg
     2401 cctgctccgt tggctggctg gtccgccggg ctcatctgct caaaccggct gtcatccatg
     2461 gttggtccta ctcgcgcact acgcgcgcca cgcccagcag gcgcgaactg atttccaggc
     2521 gctcgcgttc cgccgccgcg gtgttgatct cgaagcgcat gcgctggtcc tcgaggaaga
     2581 aacgcacgat gcccgcatgc tgcgcggcat cgccctggaa cacggtcagc acgccgttgc
     2641 cggccagcgc ctcgagcgcc accgccaggc gcgcactctc ggcgatggac agataggcca
     2701 cgtggcaact gcgcatgtcg tcgacgcgct gcacgcgccg cacctgtacc ggatgggtat
     2761 cgaccgtctt gccctgcagg ttctgttcga gcgggccggc gaacgggtcg ctgtcgagca
     2821 cgcagaacag cagcgggtcg ctcgcctgcg gcgtcttgta gcccggccac gacacgaact
     2881 tggcgaagtt gtagaggaac gccgcgcgga ccttctgctc gagatcgtcc gcgccttgcg
     2941 ccggcgacac cagcgcgagg cccagggcca gcccgccgaa taagcgcgcg atgtgcctgt
     3001 tgaatgccac ggaccggcga cctccttgtg gaccatgtct tgccggcggc cggagccggc
     3061 gcgcggtgga tggtcggttt ccttgtgcgg tgaactttag gccaagcagg gcgccggtgc
     3121 accgggggcc ggacagacgg cacggcgcat gcgccggatg cccatggtca gcccggcgcc
     3181 ggtttcagcg cggcagccgc ggcgcgcagc gcttcgagtt ccgcggcgtg gcgcaggcgc
     3241 tgttcgcggc gcagctgcgc ctcgtcgtag cgcagcttct cgatggcagt ctccggcagt
     3301 accgccggca ccgggcgcgg cttgccctgc gcgtcgacgg cgatgaaggt gagataggcc
     3361 gacaggatat ggcgccgctc gccgccgccg accttttccg catcgacctt gcagcccact
     3421 tccatcgagc tgttccacga gcggttgacg caggcgttga agatcagcac gtcgccgcgc
     3481 cgcgccggcg cgatgaagtg caccgcgtcg acgccgacgg tgacgcacac gccctgcgcg
     3541 tggcggtcgg ccaccaccgc gcagtagcgg tccatctgcg ccatgatcag gccgccgaac
     3601 acggtgtcgt tggcgttgag gtcgttggga aaaacgaagt acgcctgctc gcgcagcgcg
     3661 gaggcggaga ccggcttggc ggcgggcgtt tcagcgtgcg gcggaggagt gctcatgcgc
     3721 gccattttgc ctgcaccgca ccgcccattt tgatattatt ttttattgta tttatttaaa
     3781 aaaatacaat aaataataac aaagaattgt cgatgcggtt gaactatgtt tgctgcgcct
     3841 gatgcagcaa cggatgccgc ttggcctgcc gcagcacgcc gccgtaatag gccaggtagt
     3901 agccgatgac cagcgccagc aggcccatgg tcagcggcgt gcgcgtcatc gccgcggcca
     3961 tgccgccgtc ggtcccgtag atgacgaaca tgcggtagcc gatgcgcgcg acgaacagca
     4021 gcgacaccgc catgccaacc caggcgttgg gaatgtagaa gttcccgccc ggcgtgaact
     4081 cgtagcgcgt gagcctgagc ccgaactgcg ccagcgcggc accgagcgca agacccgctg
     4141 cggtcgacag cagcagcagg tcgtcgatgc gcgtgaagcc aatcaggatc gccagcagga
     4201 tgccgatgcg aatccacagc gtgcgctcct tcagcggctg cggaccgacg ttgcgcttga
     4261 cgcggccgta cacgctccac gccatcagcg ggccgaagat ggcgagcgtg gaagcgccgg
     4321 acaaactgtg gaccacgacg gacatggcaa gctgattcga gtttgcgcag gcgcaaccta
     4381 gcgcggcagg tccgacgcgc ccatgaggaa ctcgtccacg gcgcgcgcgg cctggcggcc
     4441 ttcgcggatc gcccacacca ccagcgactg gccgcggcgc atgtcgccgg cagcgaatac
     4501 cttgttcacg ctggtcttgt agcaaccttc accatcggtc gtggccttgg cgttgccgcg
     4561 cgcgtccttc tccactccga agccttccag cacggcggcc gcgggcgcga cgaagcccat
     4621 ggccagcagc accaggtcgg ccttcacctc gaattcggag ttgggcactt cgctcatctt
     4681 gccgtccttc cattccaggc gcgcggcgat gagtttttcc accttgccgt ccttaccctc
     4741 gaagcgcttg gtgcccactg accagtcgcg ctcgcagcct tcctcgtgcg aggacgaggt
     4801 gcgcagcttg attggccagt acggccacac cagcggcttg ttctcctggt ccggcggacg
     4861 cggcagcact tcgaactggg tgacgccggc agcgccctgg cggttgctgg tgccgacgca
     4921 gtcggagccg gtgtcaccgc cgccgatcac cacgacgtgc ttgccgtcgg cacggatctg
     4981 gtccttgacc tggtcgccgg cgtcgacgcg gttttccagg ggcaggaatt ccatggcgaa
     5041 atggatgccc ttgagctcgc ggcccggcac cggcaggtcg cggccctgct cggcaccgcc
     5101 ggtgagcagc acggcgtcga agtcttgcat caactggtcc ggcgacacgg tggtcgtggc
     5161 ccagttggtg atgttctgca gcggcttggt gcccacatag gtgctggtgc ggaaggtcac
     5221 accttcggcc ttcatctgct ccaggcggcg gtcgacgtag ctcttgtcga gcttgaagcc
     5281 cggaatgccg tagcgcagca gaccgccaac gcggtcattc ttctcgaaca cggtgacatc
     5341 gtggccggca cgtgccagct gctgcgcggc cgcgagaccc gaagggccgg aaccgacgat
     5401 ggcgaccttc cggccggtct tcaccgccgc gggctgcggc acgatccagc cgttctcgta
     5461 gcccttctcg acaatgaaca gttcgatcga cttgatgcca accggatcgc ggttgacgtt
     5521 gagcgtgcac gccgcctcgc atggcgccgg acagacgcgg ccggtgaact cggggaagtt
     5581 gttggtggag tgcagcgtgt cgagcgcggc gcggtagttg ccggtgtaca ccaggtcatt
     5641 ccagtcggga atgatgttgt tcaccgggca gccggtggtg cagaacggcg tgccgcagtt
     5701 catgcagcgc gcgccctgga tcttcgcctt ggcgtcctcg agcacgagga cgaattcctt
     5761 gtagttcttc agccgcgcat ccaccggcag gtaggactcc ttctgccggt cgaattccat
     5821 gaagcctgtg accttgccca taaatatttc ctgatgtgat gtcgcgcagc ttcaggctgc
     5881 gagcggttcc ttcttggccg gcgccggcgt tgcatgcatc tctgccagcg cgcgcttgta
     5941 ctcgtgcggg aataccttga cgaacttgcg ccggctggac atccagtcat ccagcaggtt
     6001 gcgcgcgcgt gtgctgccgg tgagcttgaa gtggcgttcg atcaggctct tgagtatggc
     6061 ctcgtcggtc tgcggcctgc cgccgcgctc gatgcggtgc cagatggcct tgtcggtttt
     6121 ctcctgctcc gccgaagtag ccagcggttc cagtgcaacc atggcgaggt tgcacttctt
     6181 ctcgaagctg ccgtcggggt catacacgta ggcgatgcca cccgacatgc cggcggcgaa
     6241 gttgcggccg gtggcgccga gcaccacgac cgtaccgccg gtcatgtact cgcagccatg
     6301 gtcgccggtg ccttccacca cggtaacggc gccggaattg cgcacggcga agcgctcgcc
     6361 ggcaaccccg ttgaagtacg cctcgcccga gatggcaccg tacagcacgg tgttgccgat
     6421 gatgatgttg tcgaccgccc agccgcggaa atcggtgttg gggcgcacga tgatgcgccc
     6481 gcccgaaagc cccttgccga cgtagtcgtt gccttcgccg accaggtcga tggtcacgcc
     6541 atgcgcgagg aacgcgccgg ccgattggcc cgcgctgccc tgcagctgga tgtggatggt
     6601 gtcatccggc aggccctcgt ggccgtagcg cttggccacc tcgccggaca gcatggtgcc
     6661 caccgtgcgg ttgtagttgc ccaccggcga gatgaacgac acgcgctcgc ctttctccag
     6721 cgcgggacgc gcctgcgcga tcagcttgtg gtccagcgcc ttctccaggc catgttcctg
     6781 cgtcagcacc tggtagcgcg gctcgtcagt cgccggctgg tagaacacgc ggctgaagtc
     6841 gagcccgcgc gccttccagt gcgcaatggc cttggcctgg tcgagcagat cggaacgacc
     6901 gatgaggtcg ttgaacttgc ggatgcccag ctgcgccatg atctggcgca cttcctcggc
     6961 cacgaagaag aagaagttca ccacgtactc gggcttgccc gcgaacttcc tgcgcagcac
     7021 cgggtcctgc gtggcgacgc cgacggaaca ggtgttgagg tgacacttgc gcagcatgat
     7081 gcagccctgg gtgaccagcg gcgccgtggc gaagcccatc tcgtcagcgc cgagcaggcc
     7141 ggcgatgacg acgtcacggc cggtcttcat ctggccgtcg gcctgcacct tgatgcgcga
     7201 gcgcaaacgg ttgagcacga gagtttgttg tgtctcggcc aggccgagtt cccacatgga
     7261 gccggcgtgc ttgacgctcg acagcggcga ggcgccggtg ccgccgtcat ggccggcaat
     7321 gaccacgtgg tccgccttgg ccttgcacac gccggcggca accgtgccca caccgacttc
     7381 ggcgacgagc ttgacggaaa tatccgcgcg tgggttggcg ttcttcaggt cgtgaatcag
     7441 ctgcgccagg tcttcgatcg agtagatgtc gtgatgcggc ggcgggctga tgaggcctac
     7501 gcctggcacc gagaagcgca gctgcgcgat gtattccgac accttcttgc cgggcaactg
     7561 gccgccttcg ccgggcttgg cgccctgggc catcttgatc tggatctggt cggcggagtt
     7621 caggtactcg gcggtgacgc cgaagcggcc agccgcaacc tgcttgattt tcgagcgcag
     7681 cgaatcgcct tcctgcagcg ggatggtggc ttcaacacgg tcgctgccca gcaccgaggc
     7741 catggtctcg ccggccttga tgctgatgcc cttgagctcg ttacggtagc gcagcgggtc
     7801 ttcgccgcct tcgccggtgt tggacttgcc gccgatacgg ttcatcgcca cggccaggct
     7861 ggtgtgcgcc tccgtgctga tcgagcccag cgacatggcg ccggtggcga agcgcttgac
     7921 gatctccttg gccggttcga cttcatcgac ggcaatggcc ttcgccggat cgatcttgaa
     7981 ttcgaacaga ccgcgcagcg tcatgtggcg cttgctctgg tcattgatga tctgcgcgta
     8041 ttccttgtag gttccgtagc tgttggcgcg cgtggaatgc tgcagcttgg cgatcgcgtc
     8101 cggtgtccac atgtgttctt cgccgcgcac gcggaacgcg tactcgccgc ccgcgtcgag
     8161 catgtttgcc agtacggggt ccttgccgaa ggcgagcttg tgcaggcgca cggcttcctc
     8221 ggccacttcg aacacgccga tgccctcgat gttcgaggcg gtgccacgga aatacttgtc
     8281 gaccagcgag cggttgaggc cgatggcctc gaaaatctgt gcgccgcagt acgacatgta
     8341 cgtgctgatg cccatcttcg acatcacctt catcaagccc ttcgcgatag ccttcttgaa
     8401 gttgtaaatg gctttttcgg gcgacagctc gccgggcaga cccttggcca tgtcggccag
     8461 cgtttccatg gcgagatacg ggtggattgc ctcggccccg tagccgccga gcagggcgaa
     8521 gtgatgcact tcacgtgccg aaccggtttc gacgaccagc ccggtggagg tgcgcaggcc
     8581 cttgctgacc aggtgctggt ggatggcgga cgtggcgagc agcgccggga tggcaacgtt
     8641 ctcggcgtcc atggtgcggt cggtgaggat cagcacgtta tggccggact tcaccgcatc
     8701 taccgccttg gcgcacagcg aggccaggcg cgcctcgatg ccttccttgc cccaggccac
     8761 cgggtagcag atgtgcagct ccttcgactt gaacttgccg cgcgtgtagg cgctgatgtt
     8821 gcgcagtcgc gcgatgtcgg ccggatcgag caccggctgc gaaacctcca ggcgcatctg
     8881 cgggttgagg ttgttgatgt cgagcaggtt cgggcgagga ccgatgaacg acaccagcga
     8941 catcaccatt gcttcgcgga tcgggtcgat cggcgggttg gtgacctgcg cgaacagctg
     9001 cttgaagtag ctgtacagcg gcttgagctt gttcgacatc accgcgggcg gcgaatcgtt
     9061 gcccatggag cccaccgctt cctcgccgtc gaccgccatc ggcgtcagca ggatgcgcag
     9121 gtcttcctgc gtgtagccga acgcctgctg gcgatcgagc agggacgcct tcggcgcttc
     9181 tacgggttcc accgccttgg cattcttcgg cagcttgagt tcgtcgagct tgagccggat
     9241 gcggttgatc tcggccttgt agggctcggc gcgggcgtag gtgtccttga gctcgtcgtc
     9301 ctcgatgatg cggccttcct cgagatcaat caggaacatc ttgcccggct gcaggcgcca
     9361 cttcttggtg atgcgcgcat caggaatcgg cagcacgccg gcttccgagg ccatgaccac
     9421 caggtcgtcg tcagtgatga tgaagcgcgc cgggcgcagc ccgttgcggt ccagcgtgcc
     9481 gccgatgtag cggccatcgg tgaaggccag ggcggcgggt ccgtcccatg gctccatcat
     9541 cgccgcgtag tactcgtaga aggcgcggcg ctgcggctcc atcagcgtgt ggttctccca
     9601 cgcctccggc accatcatca tcatggcctg cgcgatcggg tagccggcca tgaccagcag
     9661 ttcgagcgcg ttgtcgaagc aggccgtatc ggactggcct tcgtagatca gcgggaagag
     9721 cttcttgaga tcgtcgccga gttccggcga cttcatcacg ccttcgcgtg cgcgcatcca
     9781 gttgaagttg cccttcaccg tgttgatttc accgttgtgc gcgatcaggc ggtacgggtg
     9841 cgccagcggc cactcgggga aggtgttggt ggagaagcgc tggtgcacca gcgccagcgc
     9901 agagatgcag cgctcgtcgc ggagatcctg gtagtagacg ccgacctggt ccgccagcag
     9961 caggcccttg tagaccaccg tgcgcgccga catcgacggc acgaagaatt ccttgccgtg
    10021 gatcaggttc aatgcctgga tggcatggcc ggaagacttg cggatcacgt acagcttacg
    10081 ctcgagcgcg tcggtgacca tgatgtcgtc gcctcgcccg atgaagatct ggcgtatgac
    10141 cggctccttg gcacgcacgg tggccgacat cggcatgtcg cggtccactg gcacgtcgcg
    10201 ccagccgagc agcacctgcc cttcctcgcg gacggcgcgc tcgatttcct gctcgcaggc
    10261 gaggcgcgaa gcgttctcct tgggcaggaa gaccatgccg acgccatact cccccagcgg
    10321 cggcaattcg acgccctgcc tgcccatctc ttcgcgataa agctgatccg ggatctggat
    10381 cagaatgccg gcgccatcac ccatgagctt gtccgcgccc accgcgccgc ggtgctcgag
    10441 gttctccaga atctgcaagc cttgctggat gatcgagtgg gacttgccac ccttgatatg
    10501 cgcgatgaac ccgatgccgc aggcatcgtg ctcgtgctcg gaacggtaca gcccgtgcgt
    10561 ctcggccaac tgcctgtgct gcatgacaca cccctcggtt gcgagcccag gagcttattg
    10621 cactgcagca acaaagccaa gaaaaataat cggggtcaga ttctaattat tcctgtggag
    10681 tccagataca atattaattg gggacatatt aaaagatgcg gcctaattct ctcattttac
    10741 ggctttcgtc tttgaaaatg gccgaccggc acgggcgcga gtcatacgcc tcggtgtttg
    10801 cttctgtaat ccggcaacga aatcgacgtc gcccaacacc caccccttca gcgcggtgtc
    10861 ggtgagcagc gcctgcgttt gcgcggcgac gccttggccg accagttcca cgtagccggc
    10921 ttcacgggca aacggtgtgt tgcccagttg ccagtacagc ggatgagtgg tgatcagcgg
    10981 atcgctgcgc agacccgcgt agtggccata gctggaccag cggtagtccg cggcgcgcgc
    11041 aaccataccc gcacgcaccg ggttgagatc aatgtatgcc atgcaagcca gcagatagcg
    11101 ctcggtctgc actggcgtcg cacggtagcg gccttcccac aacgtgccgg tgcgctgata
    11161 acgctgattg aagtaacgca cgtagcggcg gccgacaccc tgcatcatgg ccgacagccc
    11221 ggactgcgtg gccggcgtcg ccagcaggtg gaaatggtta tccatcagca cgtagccatg
    11281 cagtgccacc ttgtcgcgcc gcgcgtgcgc cccgagcaac tcgagcagct gcagacggtc
    11341 gtcgtcatcc agaaagatag cctgccggtt gttgccccgc tggatgacgt gatgcgggtg
    11401 atgtggcacc accagccgcg gcaggcgcgc catcggcttc agcgcaacct gcgcgggtcg
    11461 tggagacgct cgtattcatc gagcgcgtag cggtccgtca tgccggcaat gtaatcggcc
    11521 accacgcgcg cacgcgcatc acggtcggcc gcagcctgcg attgcgcatg aaaatccggc
    11581 ggcatcagcc gtgcgtcgtc gatcagcgca gcgaacagat cgcacaccac gcgctgcgcc
    11641 ttcaccgtca tgcgatacac ctggtggtgc ttgtacaggt tctcgcgcag gaagcgcttg
    11701 agcacctggt tttcctgtgc gatcttttcc gaatagctga tcagcggctt ggcgcgcgcg
    11761 cgcacctcgt caatatgccg cggcctggcc ctggccagcg cggcacgcga gttctgcgtg
    11821 agatcggaca ccagcgtgtt gatgatgcgg cggatggttt cgtggcgctg ctgcttctgc
    11881 gtcacctcgc cgtagcggcg gatcacttcg tcatgatgcc ggcggaacag cggcacctcg
    11941 cgcagctgct cgatcgacag caggcgcgcg cgcaggccgt cgtcgatgtc gtgattgttg
    12001 taggcgatct cgtcggcgat gttcgccagc tgcgcctcca ggccgggctg ctggcgccgg
    12061 atgaagcgct ggcccacatc gcccagcttg cgcgcgtgtt tcagcgagca atgcttgagg
    12121 atgccctcgc gcgtctcgaa ggtgagattg agcccgcgga attccgcgta cttgtcttcg
    12181 agttcgtcga ccacgcgcaa cgactgcagg ttgtgctcga agccgccata cggcttcatc
    12241 gccgcattga gcgcatcctg gccggcgtgg ccgaacggcg tgtggccgag atcgtgcgca
    12301 agcgagatcg cctcggccag atcctcgttg agcgacaacg tgcgcgcgat agtgcgcgcg
    12361 atctgcgcca cctcgatgga atgcgtcagc cgcgtgcgga acaggtcccc ttcatgattg
    12421 acgaatacct gcgtcttgta ttccagccgg cggaacgccg cgctgtgcac gatgcggtcg
    12481 cggtcgcgct ggtactggct gcggtaggaa ggcgcgcttt ccgcgtggcg ccggccgcgg
    12541 ctgtgctcgt cgcttgcggc gcagggcgcc agctggaagg tcatgcctga ttagaactta
    12601 aagcctttgc agccgccacc gcctgccatg ttttgttgtt attaaatatt gtttaatttt
    12661 taatttaaat gataatttat cacatcaaat ggcaaggtgc ggtatggctg agttcaggcc
    12721 tccgagaagg ccgcgagcgt cgccgcaagc gccgttgcat cgtagtccgc gctcagcatc
    12781 gcatcgccca ggcgcctgag cagcaccagg cgcaattttc cgccggccac tttcttgtcg
    12841 agcttcatca gttccatgaa gtgctgtgcg cgcatgcccg gcggcgggcg caccggcagg
    12901 ccggcgcggg ccaccagatg gcgcgagcgc tgcgcatcgc tgtcggagat ccagccgagc
    12961 ctggccgaca ggtcggccgc catgcacagg ccggcgccga ccgcttcgcc atgcagccaa
    13021 ctgctgtagc cggtgtaggt ctcgatggcg tggccgaagg tgtggccgag attgagcaag
    13081 gcgcgcgggc caccgctgat gctctcgcgc tcgtcgacgc cgacgatctg cgccttgagt
    13141 tcgcagcagc ggcgcacggc ctgtgccagc gcccgcggct cgagcgcgag cagcgcatcg
    13201 agattctgat cgagccaatc gaagaacagc gcgtcggcca gcatgccgta cttgatcacc
    13261 tcggccagcc cggcggatag ctcgcgccgc ggcagcgtgg tcagggtggc caggtcggcc
    13321 actaccgcgc gcggctggtg aaacgcgccg atcaggttct tgccgcgcgc atggttcacg
    13381 ccggtcttgc cgccgaccga tgaatccacc tgggcaagca gggtggtggg aatctgcacg
    13441 aagtcgatgc cgcgctggta aatggccgcg cagaagcccg ccaggtcgcc gatgacgccg
    13501 ccgccgaggg ccgccagcat gccgtcgcgc ggcaaacgcg cggcgagcag ccagtcgagc
    13561 acgtgctcgg cgttgttcca ggtcttctgc gtttcgccgg caggcagcac cagcgcatca
    13621 gccgcgtcga gcttgagcgt gcgcagcagc gcgggcaggt agtgcgccgc cacctgcgca
    13681 tcgctgacga tcttcagcgg acggccgtgc gtttcggcga agttcgcggc gtcggccagc
    13741 agcccattgc cgatgcggat cgggtagctg cgggtgccga gctggacggt gagcgtttcc
    13801 ataggactgc gcgcgggcct gtccgcgcgg cgcgacatgc taaagcaatt cgccgctcag
    13861 aatgcggtgg cgggacgcgc caggtgatcc tcgatgtcgc gcaccagcag gcgtgcattg
    13921 cggccgtcgg tggccagcac cagatcggcg atctcacggt acagcgcttc acgccgggca
    13981 aacaactcct ccagcacttc gcggcgatca tcggcgcgcg acagcagcgg gcgattgtcg
    14041 ctgcgctcgg tacgctgcat ctgctggttg atgctggcgt gcaggtacac caccagtccg
    14101 cgggcagaca gcatctggcg gttctcagga tcgagcaccg aaccgccgcc ggtggccagc
    14161 acgatgttgc tgcgctgggt gatctcggcc agcacctgct tctcgcgctt gcggaagccc
    14221 tcttcgcctt ccttctcgaa gatgtaggcg atatcgacgc cggtccgcgc ctcgatctcg
    14281 tggtcgctgt cgaggaactc cataccgcgc acttcggcca ggcgccggcc gaccgtggtc
    14341 ttgccggcgc ccatcgggcc gatcaggaag atgttgctct tgctgcccat gtatccgggc
    14401 tcacccctgt ttcagtggac cttgagtcct tcctgcagga tcttgggcgt gacgaagatc
    14461 agcagctcgc gcttggtgtt gttgttgtcg ctgttcttga agaagttgcc gagcagcggc
    14521 aggtcgccga ggaacggcac cttggaagtg tcgtgttcct gggtcgagtc gaacaccccg
    14581 cccagcacca cggtttcacc ggtgcgcacc agcacctgcg tcgacaggcg ccgcgtgtcg
    14641 atcgacggtg cggtacccag acccacggca acgttggcgc cctgggtatc gtcggtgacg
    14701 gtcaggtcca tcaggatgcg gctgtccggc gtgatctgcg gattcaccgt cagcgacagc
    14761 acggccttgc ggaaggcgac cgtggccgcg ccgctggaag ccgcttccag gtacggaatt
    14821 tccgtaccct gctccaccac tgcttccttg ccgttcgcgg tgatgacgcg cggcgtcgac
    14881 agcacctcgc cacgcccctc gatctgcagc gccgacaact ccatgtcgac caggtagttg
    14941 gcgcccagga tggccagcgc aaagctgccc ggattggccg aagcggtcgg caggttgacg
    15001 ttgaagcggt tggcctgcga aggcagcgtc accgcggtgc cgccggtgga cagcgcactg
    15061 ttgatcatgg tgtcggtgcc cgaactgctg cccgtcgtgg cgagcaggtt attgccgcca
    15121 ttggcggtgc cgaccgaagt gacgccgaag cgtgcaccga gttccttgcg gtagtcgtcg
    15181 tgggcaatga cgatgcgcga ctcgatcaga acctggcgta ccggcacgtc gagctgcgcc
    15241 accagggcgc ggatctcggc cagcttttcg cgcgtttcca gcacgatcag ctggttggtg
    15301 cgctcgtcga ccgtcacatg tccgcg
//
LOCUS       DASZZC010000045         5515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_68670,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000045 DASZZC010000000
VERSION     DASZZC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68670"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /gene="rlmJ"
                     /locus_tag="VHE37_01145"
     CDS             complement(<1..333)
                     /gene="rlmJ"
                     /locus_tag="VHE37_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HVT34160.1"
                     /translation="MHYQHRYHAGNFADVFKHVVLCALLQALNRKGTPWCCLDTHAGA
                     GLYDLRDESSARTGEWQDGIARLDQAGDAPALIADYLRIVRGAGDGKYPGSPLVACAL
                     ARPQDRLVL"
     gene            complement(491..1903)
                     /locus_tag="VHE37_01150"
     CDS             complement(491..1903)
                     /locus_tag="VHE37_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34161.1"
                     /translation="MRRPTAALALAACLPCAAWAHSFGQIYNLPVPLWLYTYGAAAAL
                     LLSFVVLGWWMTAAPPEPQLRAAADPPRLLTGRVARATSVACLLLTIIAGFAGTSNPY
                     ANIGMTLFWIWFALGLAYASALLGNFYAAINPWRVLIEWIERFRPEFFSGRARASPEG
                     SVYIALVFYAAFVGFELLGHPSPFSLSVALVVYTLCNLAGAWWLGQPAWFARGEFFGV
                     LFALYARMAPGRGGVLRERAADFSVLLFVLFMLSSTAYDGVRETLPWSMLYWKQLAPL
                     LTPWVGNDIVQSFPILRAIHHVWEWLTLLASPLLYLGLYLFFIELSRRCAGSARSTRE
                     LALDFAYTLLPIVLVYHLAHYFSLLVTQGPQLLRLLSDPFGRGWDVFGTARWLRTPII
                     LPAGWVWHTQVGLIVAGHVASVYLAHVQALRIFREPRAALRSQLPMLVLMVAFTTLGL
                     WILAQPIQSGQVVVPAAPQR"
     gene            complement(1906..2331)
                     /locus_tag="VHE37_01155"
     CDS             complement(1906..2331)
                     /locus_tag="VHE37_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34162.1"
                     /translation="MNNRALLFVVIGAAVLAGLFVLMKPAAEPQAVPAAPAAAAAPDA
                     AAPVVVQPRVHAFELVVKDRRLVSGPQLLSVNQGDAVTIGIVSDAAEELHLHGYNLKL
                     ELAPNQHAVLAFNATHSGHFEYELEHARVELGALEVQPQ"
     gene            complement(2407..4005)
                     /locus_tag="VHE37_01160"
     CDS             complement(2407..4005)
                     /locus_tag="VHE37_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34163.1"
                     /translation="MSSHRVAPRRRWNSTWVVLLLAGCTAGQDSAVPGAPADMGKADV
                     TPAVFEGPVPRAVCGANSLPETDIQGRVSIDDRSSGRSQQGYRCNLELVGQYQGQGTT
                     WVSQSYKNCAYHSQTFPTSLQGPSPGVQVVDVSDPKHPVLAGTLTSPAFLGNTWESLK
                     VNEARGLLAGVQVGIGEGAVFFDVYDIGTDCLHPKLLNSVGPTQLSIPADSIGHEGNW
                     SPDGNTYWSTSLAGGIVTAIDVSDPALPRALFVGNAGIVNHGFELSADGNRLYLANVA
                     PEGLDILDVSEIQSRKLAPQMHKLGSVTWTDGSTGQHSIPVSWNGKPYLVFVDEGGNG
                     AARIIDISDETAPVVVSKLKLEIHMPEYAEQRTADLAGNGSFGYEGHYCTVDRRENPT
                     AIACGYFQSGVRVFDVRDPMNPHEIAYYNPPAQVGKSAQLTGSEHANGLLQVYAPANL
                     TADWCSSPPRFVEQADGERQLWVTCQDNGFMVLKFTNGSYPVADVAPVVTPVVSAPST
                     GRYGAALAWSLLLPLFAAALRRRR"
     gene            complement(4153..5397)
                     /locus_tag="VHE37_01165"
     CDS             complement(4153..5397)
                     /locus_tag="VHE37_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HVT34164.1"
                     /translation="MKSKQYPVTALALMAACTVAQAAEAPSTIPVTALRSDAAPAAAP
                     GGMATPDLKYSWGGYIKLDSMYSHFSDGDVATPGSARDFYVPGSIPVKSTPEQHSFFD
                     AHAKETRLWFRSDGTWGDHKVGAYVEMDFLVNPGAGQERTTNAYNPGLRRAYLTFDNW
                     LAGQDWSTFQNTGALPEGLDFIGPTESTVFVRQPQLRYTEGSFQVALENPETTLTAHG
                     GASINNVTGDGTLPDLVLRYNAKTGFGDFTIAGIGRQLKANQTALGATAVANGSAAGY
                     GASASGKVPLGDDDLRFMVSGGKGIGRYVGINTIDDAVVDANGGLQSLGLVAGFVAYR
                     HPWSEQWRSSLTLSTLHANNDSANTGVDVTKSVYSGHLNLLYSPLPKITFGGELTYAK
                     RKLESGLDGDMSRLQLSAKYIF"
BASE COUNT          974 a         1807 c         1873 g          861 t
ORIGIN      
        1 cagcaccagc cggtcctgcg gccgcgccag cgcgcacgcc accagcggcg atcccggata
       61 ttttccgtcg cccgcgccac gcacgatgcg caggtagtcg gcgatcagcg ccggcgcgtc
      121 gccagcctgg tccagccgcg cgatgccgtc ctgccactcg ccggtgcgcg cggaagattc
      181 atcgcgcagg tcgtacaagc ccgcgccggc atgcgtgtcg agacagcacc acggcgtgcc
      241 cttgcggttc agcgcctgca gcagcgcgca cagcaccacg tgcttgaaca cgtcggcgaa
      301 gtttcctgcg tggtagcgat gctggtaatg cacgcgcgca ttctagtgaa ccactgaaca
      361 agcgtcgcga gcgatgacag gttgcgcgag gacggagcgc cgcaagccga gcatacgtac
      421 gagtatgtga gcattgcgag taccacccgc gcgcaagatg tcgagcgcag cagatttgtt
      481 cagcggttca ctatcgctgc ggagcagcag gaacgacgac ctgcccggac tggatcggct
      541 gggccaggat ccacagtccc agcgtggtga acgccaccat cagcaccagc atcggcagct
      601 ggctgcgcag cgccgcgcgc ggctcgcgga agatgcgcag cgcctgcacg tgtgccaggt
      661 acacgctggc gacatgcccg gcgacgatca gcccgacctg cgtgtgccac acccagccgg
      721 ccggcaggat gatcggcgtg cgcagccagc gcgcggtgcc gaacacgtcc cagccgcggc
      781 cgaaaggatc ggacagcagc cgcagcagct gcgggccctg ggtcaccagc agcgagaagt
      841 aatgcgccag gtggtagacc agcacgatcg gcagcagcgt ataggcgaaa tccagcgcga
      901 gctcgcgcgt gctgcgcgcg ctgccggcgc agcggcgcga caactcaatg aagaacaggt
      961 acaggccgag gtagagcagc ggcgacgcca gcagcgtcag ccattcccag acgtggtgaa
     1021 tggcgcgcag tatcgggaag ctctgcacga tgtcgttgcc cacccacggc gtgagcagcg
     1081 gcgccagctg cttccagtag agcatcgacc atggcagggt ctcgcgcacg ccgtcgtacg
     1141 cggtcgatga cagcatgaac agcacgaaca gcagcacgga gaaatccgcc gcgcgctcgc
     1201 gcagcacgcc gccacggcca ggtgccatgc gcgcgtagag cgcaaacagc acgccgaaga
     1261 attcgccgcg cgcgaaccac gccggctggc ccagccacca cgcgcccgcg agattgcaca
     1321 gcgtgtagac gaccagtgcg accgacagcg agaacggcga aggatggccc agcagttcaa
     1381 agccgacgaa ggcggcgtag aacaccagcg caatgtagac actgccctcc ggcgacgcgc
     1441 gggcgcggcc ggaaaaaaac tccggccgga atcgctcgat ccattcgatc agcacgcgcc
     1501 agggattgat cgcggcgtag aaattcccca gcagcgcact ggcgtaggcc aggcccagcg
     1561 cgaaccagat ccagaacagc gtcatgccga tgttggcgta gggattcgag gtgccggcga
     1621 aaccggcaat gatggtgagc agcaggcagg ccacgctggt ggcgcgcgca acgcggccgg
     1681 taagcagccg cggcgggtcc gccgctgcgc gcagctgcgg ctcaggcggc gctgccgtca
     1741 tccaccatcc cagcacgacg aaggacagca gcagcgccgc agcggcgccg taggtgtaca
     1801 gccacaacgg caccggcagg ttgtagatct ggccgaagga atgcgcccac gccgcgcacg
     1861 gcaggcaggc cgccagtgcc agcgctgctg taggccgccg catgtttact gcggctgcac
     1921 ttcgagcgcg cccagttcga cgcgcgcgtg ctccagttcg tactcgaaat ggccgctgtg
     1981 cgtggcattg aacgccagca ccgcgtgctg gttcggcgca agttcgagct tgaggttgta
     2041 gccgtgcagg tgcagttcct cggccgcgtc gctgacgatg ccgatggtca cggcgtcgcc
     2101 ctggttcaca ctgagcagct gcggccccga caccaggcgg cgatccttca ccaccagttc
     2161 gaaggcatgc accctgggct gcacgactac cggcgccgct gcgtccggcg cagccgcggc
     2221 ggccggcgcg gcaggcacgg cttgcggttc cgccgccggc ttcatcagca cgaacaggcc
     2281 cgccagtacg gcggcaccga tgacgacgaa cagcagggcg cggttgttca tgggcggaaa
     2341 tcgcaacggg tggctgcgga ggcgggcgcc tagcgtagcg tgcgcgaacc ttgcgcggtg
     2401 cgcgccttac cggcgccggc gcaaggccgc tgcgaacagc ggcagcagca gcgaccacgc
     2461 cagcgcagcc ccgtagcggc cggtggacgg cgcggaaacc accggcgtca cgaccggcgc
     2521 gacgtcggcc accgggtacg aaccgttggt gaacttgagc accatgaagc cgttgtcctg
     2581 gcaggtcacc cacagctgcc gctcgccatc ggcctgctcg acgaagcgcg gcggcgacga
     2641 gcaccagtcc gcggtgaggt tggccggggc gtagacctgg agcaggccgt tggcatgttc
     2701 ggagccggtc agctgcgcgc tcttgcccac ctgcgccggc gggttgtagt acgcgatctc
     2761 gtgcgggttc atcgggtcgc gcacgtcgaa cacgcgcacg ccgctctgga aataaccaca
     2821 ggcgatcgcg gtcgggttct cgcggcggtc gaccgtgcag taatggcctt cgtagccgaa
     2881 gctgccattg ccggcgaggt cggcggtgcg ctgctcggcg tattccggca tgtggatttc
     2941 cagcttgagc ttggacacca ccaccggcgc ggtctcgtcg ctgatgtcga tgatgcgcgc
     3001 ggcgccgttg ccgccctcgt cgacgaacac caggtagggc ttgccgttcc agctgacagg
     3061 aatcgagtgc tggccggtgg aaccgtcggt ccaggtgacg ctgccgagct tgtgcatctg
     3121 cggcgccagc ttgcgcgact ggatttccga gacgtcgaga atgtccaggc cttccggcgc
     3181 gacattggcc aagtacaggc gattgccgtc ggcggaaagt tcaaagccgt ggttgacgat
     3241 gccggcgttg ccgacgaaca gcgcgcgcgg cagtgccgga tcgctcacat caatggcggt
     3301 gacgatgccg ccggccaggc tggtggacca gtaggtattg ccatcaggcg accagttgcc
     3361 ctcatggccg atggaatcgg ccgggatcga cagctgcgtc ggccccacgc tgttgagcag
     3421 cttcggatgc aggcagtcgg tgccgatgtc gtacacgtcg aagaacaccg cgccttcgcc
     3481 gatgccgacc tgcacgccgg cgagcaggcc gcgtgcctcg ttgaccttca acgattccca
     3541 ggtgttgccg aggaacgccg ggctggtgag cgtgcccgcc agcaccgggt gcttcggatc
     3601 gctcacgtcc accacctgga cgccgggcga cggcccctgc agcgaagtcg ggaaagtctg
     3661 cgagtgatac gcgcagttct tgtagctctg gctgacccag gtggtgccct ggccctggta
     3721 ctggccgacc agctcgagat tgcagcggta gccctgctgc gaacggccgc tgctgcggtc
     3781 atcgatagac acgcggccct ggatatcggt ctccggcagc gagttggcgc cgcagaccgc
     3841 gcgcggcacc ggaccttcga ataccgccgg cgtcacgtcg gccttgccca tgtcagccgg
     3901 cgcgccgggc actgcggaat cctgccccgc cgtgcatccc gccagcagca gcacgaccca
     3961 tgtggaattc cagcgcctgc gcggcgccac gcgatgactg ctcatgtctc ctccccgcat
     4021 gatcggcgca tggacctgcg cctttttcgt acaccggaaa gtgtgctgaa ttccggcgat
     4081 ttgtaaatta gaccttggtc gcatcaagcc ggaaaagccg cagcatcctg ccgcggcgta
     4141 tccgtcacaa cctcagaaga tgtacttggc cgagagttgc aggcgcgaca tgtcgccatc
     4201 taggccgctc tccagcttgc gcttggcgta agtcagttcg ccgccaaatg tgatcttcgg
     4261 cagcggcgaa tacagcaggt tgagatgccc gctgtagacc gacttggtga catcgacgcc
     4321 agtgttggcg ctgtcgttgt tggcatgcag cgtcgacagc gtgagcgagg aacgccattg
     4381 ctcggaccac gggtgacggt aagccacgaa accggcgacc aggcccagcg actgcagacc
     4441 gccgttggcg tcgaccaccg cgtcgtcgat ggtgttgatg ccgacgtagc ggccgatgcc
     4501 cttgccgccg ctcaccatga agcgcaggtc gtcatcgccc agcggcacct tgcccgatgc
     4561 gctggcgcca taaccggcgg cgctgccgtt ggccacggca gttgcgccga gcgcggtctg
     4621 gttggccttg agctggcgac cgatgccggc gatggtgaaa tcgccgaagc cagtcttggc
     4681 gttgtagcgc agcaccagat ccggcagcgt gccgtcgccg gtgacgttgt tgatggaagc
     4741 gccgccatgt gcggtcagcg tggtctccgg gttttccaac gccacctgga agctgccctc
     4801 ggtgtagcgc agctgcggct ggcgcacgaa cacggtggat tcggtggggc cgatgaagtc
     4861 gaggccctcg ggcagcgcgc cggtgttctg gaaggtgctc cagtcctggc cggcaagcca
     4921 gttgtcgaag gtcaggtaag cacgccgcag gccggggttg tatgcattgg tggtgcgctc
     4981 ctgcccggca ccgggattca ccaggaaatc catttccacg taggcgccga ccttgtggtc
     5041 gccccaggtg ccgtcgctcc tgaaccacag gcgcgtttcc ttggcatgcg cgtcaaagaa
     5101 gctgtgctgc tcgggcgtcg acttcaccgg gatcgagccg ggcacgtaga aatcgcgcgc
     5161 cgagccgggc gtggcgacgt caccatcgga gaaatggctg tacatgctgt cgagcttgat
     5221 gtagccgccc cagctgtact tgaggtcggg cgtcgccatg ccgcccggcg ctgcggccgg
     5281 cgcagcatcg ctgcgcagcg cagtcacggg gatggtggaa ggggcttcgg ccgcctgggc
     5341 gacagtgcat gcagccatca acgccaatgc ggtgacggga tattgcttgg atttcatcag
     5401 ggactcctgt gcggacgtgc gcagcatgcg tggaccgccg ggctcctgct attagccgaa
     5461 ggtcgtaatc gggccgcttt acgaccaagg tcttatgcgc agcaaccccg aagtg
//
LOCUS       DASZZC010000046         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_323784,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000046 DASZZC010000000
VERSION     DASZZC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323784"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..780)
                     /locus_tag="VHE37_01170"
     CDS             complement(199..780)
                     /locus_tag="VHE37_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HVT34165.1"
                     /translation="MPASPPRLILASGSRYRAELLARLRLSFEAMASNVDETPHRGEP
                     VRALTQRLALAKARALADAHPGAWVLGSDQAAIADGRLLGKPGTHARAVEQLQFLSGK
                     KVEFHTAVVLAGERVLSGADITTVHFRELASDEIERYLAAEPAYDCAGSFKCEGLGIT
                     LFERIESDDPTALIGLPLIAVRRLLADAGWPLP"
     gene            849..1355
                     /locus_tag="VHE37_01175"
     CDS             849..1355
                     /locus_tag="VHE37_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HVT34166.1"
                     /translation="MGIPATVSASGAVAKAQRYEGDLSLTAMPRLAQLVRPEAHAVQV
                     QLVADDKSGYPRMHGEVSGTLPLECQRCGRAFDWPLQAAIDLRLVRDEQHEHDLLGDC
                     DPYLVRDDQLPLRELIEDEVLLALPMLARCESCENSVNAAPAAVQEGPAVRRENPFAA
                     LKDKLKHS"
     gene            1386..1583
                     /gene="rpmF"
                     /locus_tag="VHE37_01180"
     CDS             1386..1583
                     /gene="rpmF"
                     /locus_tag="VHE37_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HVT34167.1"
                     /translation="MAMQKSKKSRQMRGHRRSHDALAKPALSVDQTSGETHLRHHVTA
                     DGYYRGKKVIDKSVPEVSEQS"
     gene            1604..>2250
                     /locus_tag="VHE37_01185"
     CDS             1604..>2250
                     /locus_tag="VHE37_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HVT34168.1"
                     /translation="MTAPVTLAIDAMGGDHGYPVIVEASLAALKAHPEAELILVGDEP
                     KLARALAERKHARGPRLSVHHASETVEMDESPSKALRNKKDSSMRVAINLVKEGRAQA
                     AVSAGNTGALMATARFVLKTLPGIDRPAIVSAIPSVNGHVHMLDLGANAECSAEQLVQ
                     FAVMGSALVTAVHGIERPSVALLNIGEEEIKGNEVIKQAAALLQGSTLNYVGFVEG"
BASE COUNT          407 a          722 c          777 g          344 t
ORIGIN      
        1 atcacccgga gcgcctgctc ggggctggcg cggtgggcct gatcgagaac gtggccgcgc
       61 tgcctgcctg gttggcgcag cgctgagcca ttttccgccc aaccgcaccc cggttttcga
      121 tgttattaaa tatataatta aattaaaata ataatattta ttataacaag caaaatgccg
      181 gtgcggtgac cccaaaattc acggcagcgg ccacccagca tcggccagca ggcggcgcac
      241 cgcgatcagc ggcaggccga tcagcgcggt cggatcgtcg ctctcgatgc gctcgaacag
      301 cgtgatgccc aggccttcgc acttgaagct gccggcgcag tcataggcag gctcagcggc
      361 gaggtaacgc tcgatctcgt cactggcgag ctcgcggaaa tgcacggtgg tgatgtccgc
      421 cccgctgagc acgcgctcgc cggcgaggac gacagcggtg tggaattcca ctttcttgcc
      481 ggagaggaat tgcagttgct ccaccgcgcg cgcatgcgtg cccggcttgc cgagcagccg
      541 gccatcggca atcgccgcct ggtccgagcc gagcacccat gcgcccgggt gcgcatcggc
      601 cagggcgcgc gccttggcca gcgccaggcg ctgggtgagc gcacgcaccg gttcgccccg
      661 atgcggcgtc tcgtcaacgt tggaagccat cgcttcgaaa gacaggcgca ggcgcgccag
      721 cagctcggcg cggtagcgcg agcccgaggc caggatcagg cggggcgggg aggcgggcat
      781 gcgttttgac ttgccggaga gcgctcccta ttatgcgcgc cccgcgcggg tagccgtcgg
      841 gatctgccat gggtattcca gccacagttt cggccagcgg cgcagtcgcc aaggcgcagc
      901 gctacgaggg cgacctgtcg ctgacggcca tgccgcggct ggcccagctg gtacggccgg
      961 aggcgcatgc ggtccaggtg cagctggtgg cggatgacaa gtccggctac ccgcggatgc
     1021 atggcgaggt cagcggcacg ctgccgctgg agtgccagcg ctgcggacgc gcgttcgact
     1081 ggccgttgca ggcggccatc gatctgcggc tggtgcgcga cgagcagcac gagcacgacc
     1141 tgctgggcga ttgcgatccg tacctggtgc gcgatgacca gttgccgctg cgcgagctga
     1201 tcgaggacga ggtgctgctg gcgctgccga tgctggcgcg ctgcgaatcg tgcgaaaata
     1261 gcgtcaacgc tgcgccggca gcggtacagg aaggaccggc agtgcggcgc gaaaatcctt
     1321 tcgcggcgct caaggacaag ttgaagcata gttaagccgg gccgaaagcc ggcggagtta
     1381 ccaagatggc gatgcagaaa tcgaagaagt cccggcagat gcgcggccac cgccgctcgc
     1441 acgatgcgct ggccaagccc gcgctgtcgg tcgaccagac ctcgggcgaa acccacctgc
     1501 gtcaccacgt gaccgccgat ggctactacc gcggcaagaa ggtcatcgac aagtcggttc
     1561 cggaagtcag cgaacaaagc tgatcccttc cgcgggcgcg gcgatgactg cacccgttac
     1621 cctcgccatc gacgccatgg gcggcgatca cggctacccg gtgatcgtcg aagcgtcgct
     1681 ggccgcgctc aaggcgcatc ccgaagctga actgatcctg gtgggcgacg agcccaagct
     1741 ggcgcgcgcg ctggccgagc gcaagcacgc gcgcgggccg cgcctgtccg tccaccatgc
     1801 cagcgagacg gtggagatgg acgagtcgcc gtccaaggcg ctgcgcaaca agaaagactc
     1861 ctcgatgcgc gtggccatca acctggtcaa ggaaggccgc gcgcaggctg ccgtgtcggc
     1921 cggcaacacc ggcgcgctga tggccaccgc gcgcttcgtg ctgaagaccc tgccgggcat
     1981 cgaccgcccg gccatcgtct cggccatacc ctcggtcaac ggccacgtgc acatgctcga
     2041 cctgggcgcc aatgccgaat gcagcgcgga gcagttggtg cagtttgcag tgatgggcag
     2101 cgcgctggtc accgcggtgc atggcatcga acggccgagc gtggcgctgc tcaacatcgg
     2161 cgaggaagag atcaagggca acgaggtgat caagcaggcg gccgcgctgc tgcagggttc
     2221 gacgctcaac tatgtgggtt tcgtcgaggg
//
LOCUS       DASZZC010000047         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_196749,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000047 DASZZC010000000
VERSION     DASZZC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196749"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..2225)
                     /locus_tag="VHE37_01190"
     CDS             complement(174..2225)
                     /locus_tag="VHE37_01190"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HVT34169.1"
                     /translation="MSAPYPRLLAPLDLGFTQLRNRVLMGSMHTGLEDRKKNFPRLAE
                     YFAERARGGVGLIVTGGFSPNIEGWLLPLASKMSTSAAARAHKPITDAVHREGGKIAM
                     QILHSGRYGYHPLQVAPSKIKSPITPFNPRELSTAGVERQIKAFVRAGVLAREAGYDG
                     VEVMGSEGYFINQFLVTHTNRRIDRWGGSYENRMRLPVEIVSRMREAVGKDFIIVYRL
                     SMLDLLPDGSTWDEVVMLAKAIEKAGATIINTGIGWHEARVPTIATSVPRAAFAWVTK
                     KLKGEVSIPLCTTNRINAPEVAEQILADGCADMVSMARPLLADADFVNKAAAGKADAI
                     NTCIACNQACLDHTFKNKVASCLVNPRACHETQLNYIATPKKKRVAVVGAGPAGLAAS
                     TVLADRGHHVELFDAASEIGGQFNMAKRIPGKEEFHETLRYFRNKIQSSGVKLHLSTR
                     VSAAQLLEGKYDDIVLATGVTPRNPRIPGQDEQIKTGKVLSYIDVLLYKKPVGKRVAV
                     IGAGGIGFDVSEYLVQDGHSPTLNLQEWLAEWGVADPAAARGGVAGVKPEIAPPAREV
                     LLLQRKASKHGAGLGKTTGWIHRSTLKMKRVEMIGGVNYESIDARGILVSFGEKRERA
                     TLLEVDNIILCAGQEPLRELHQPLLDAGVSVHLIGGADVAAELDAKRAINQGSRVAAE
                     I"
     gene            2289..2990
                     /locus_tag="VHE37_01195"
     CDS             2289..2990
                     /locus_tag="VHE37_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVT34170.1"
                     /translation="MRVLMNFTAKMFLTAAALLGGTAFAGVYKWVDADGRVHYGDRPP
                     ANAGAQAVSVGAIPSGLEERLRALDPDFSITRLSGDLKETLVCGEFDAHGVRDAQPRF
                     LPALKASGLAEVLPDEGQPTVRGQRAPFYDRDRCPPPAPQQRLAQRSYRIQFTPDAIA
                     AYGNAGSGRGCRCNAPTAATCRSRRCIARAAASTSRNSGSCSRGRRRLHHRRWKLRRS
                     RSATTSAHGCGAAQR"
     gene            2990..4132
                     /locus_tag="VHE37_01200"
     CDS             2990..4132
                     /locus_tag="VHE37_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVT34171.1"
                     /translation="MGLAVAVGAAKLVTLVQEQAQIPHALAAVEIYRNEIAARQRNRY
                     LLSSAFPAANWQLGLPAPDDAHWRGFGSVNLVADGRVVFVHAGSGVLDPSFKLAIFAP
                     PDTWAPQCGGVGLGARAQQALARYCEWNRLSVDVPPPPVQPPAPTQAPQGPSETEHEQ
                     FSERYEQGKTPLIDAADHGDEAQLRKLLAAGTEVDGRDRYGSTALMYAARAGCAACVQ
                     ALLDAHADIHARNTNGAGVWSWALMQPAGAPSAATPDERLHTYELLLSHGADANLDAF
                     DPSGRALLHRAADTDDAALAAFLLAHGAALELRDAQGRTPLMHAAVADGGDEVLELLI
                     KAGARASARDNDNRPVIELLRLEYHGPHVEHRTALLRDAMAYPSRH"
     gene            4305..>4998
                     /locus_tag="VHE37_01205"
     CDS             4305..>4998
                     /locus_tag="VHE37_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34172.1"
                     /translation="MQPETRYVRTPRGDIAYQVVGEGKRDLVYLSGMVTHLDLTWDFP
                     EAERYLTRLASFSRLILFDRRGTGISEAIAQDEQLSWSGWLDDLRAVLDATGSKSAAL
                     LAERDAGSVAMQFAASEPERVSALILANTSARYLAAPDYPCGESPQVAEQLVRTVHEL
                     WGSERMTALAMPSRADDTNFLRVSARFQRASATPSTAARQYRFFFSRDAREVLPQISC
                     PTLVMHRREYPFL"
BASE COUNT          765 a         1693 c         1710 g          830 t
ORIGIN      
        1 cgatgcccac cagcggcagc gtatggtcga cgaagtaggg gtaggccatc agcgcaatac
       61 cgcacaccag cggcacgatt ttctgctggc gcttgccata gatgaagtag cccagcccga
      121 tcgagctgaa cagcaggccc cacagcagct gcgcctggtt catctgcccg cgttcagatt
      181 tccgccgcca cccggctgcc ctggttgatg gcgcgcttgg cgtccagctc cgccgccacg
      241 tcggcgccgc cgatcaggtg tacggacacg cccgcgtcga gcagcggctg gtgcagctca
      301 cgcagcggct cctggccggc gcacaggatg atgttgtcca cttccagcag cgtggcgcgc
      361 tcgcgcttct cgccgaagct gaccaggatg ccgcgggcat cgatcgactc gtagttgacg
      421 ccgccgatca tctccacgcg tttcatcttc agcgtgctgc ggtggatcca gccggtggtc
      481 ttgcccaggc cggcgccgtg cttgctggcc ttgcgctgga gcagcagcac ctcgcgcgcg
      541 ggcggggcga tctccggctt cacgcccgcc acgccgccgc gcgccgcggc cggatcggcc
      601 acgccccatt cggccagcca ttcctgcagg ttcagcgtgg gcgagtggcc gtcctgcacc
      661 aggtattcag acacgtcgaa gccgatgccg ccggcgccga tcaccgccac gcgcttgccg
      721 accggtttct tgtacagcag cacgtcgatg tagctcagca ccttgccggt cttgatctgc
      781 tcgtcctggc cggggatgcg cgggttgcgc ggcgtgacgc cggtggccag cacgatgtcg
      841 tcgtatttac cctcgagcag ttgcgccgcg ctgacgcggg tggacaggtg cagcttcact
      901 ccgctggact ggatcttgtt gcggaaatag cgcagcgtct cgtggaattc ctccttgccc
      961 gggatgcgct tggccatgtt gaactggccg ccgatctcgc tggcggcgtc gaacagttcg
     1021 acatggtggc cgcggtcggc cagcacggtg gacgcggcca ggcccgcggg tccggcgccg
     1081 accactgcca cgcgtttctt cttcggcgtg gcgatgtagt tgagctgcgt ctcatggcag
     1141 gcgcgcgggt tcaccaggca ggacgcgacc ttgttcttga acgtgtgatc gaggcaggcc
     1201 tggttgcagg cgatgcaggt gttgattgcg tcggccttgc cggcagcggc cttgttgacg
     1261 aagtcggcat cggccagcag cggccgcgcc atgctgacca tgtcggcgca gccgtcagcc
     1321 aggatctgct ccgccacttc cggcgcattg atgcggttgg tggtgcacag cgggatcgac
     1381 acttcgccct tgagcttctt cgtcacccag gcgaatgccg cgcgcggcac actggtcgca
     1441 atcgtcggca cgcgcgcctc gtgccagccg atgccggtgt tgatgatggt ggcgccagcc
     1501 ttctcgatcg ccttggccag catcaccacc tcgtcccagg tgctgccgtc gggcagcagg
     1561 tcgagcatcg acaggcggta gacgatgatg aagtccttgc ccaccgcctc gcgcatgcgc
     1621 gaaacgatct ctaccggcag ccgcatgcgg ttctcgtagc tgccgcccca gcggtcgatg
     1681 cgccggttgg tgtgcgtgac caggaactgg ttgatgaagt agccctcgct gcccatcacc
     1741 tccacgccgt cgtagcccgc ctcgcgcgcc agcacgccgg cgcgcacgaa ggccttgatc
     1801 tgccgctcga cgccggcggt ggacagttcg cgcggattga acggcgtgat cggcgacttg
     1861 atcttgctcg gcgccacctg cagcgggtgg tagccatagc ggccgctgtg caggatctgc
     1921 atggcgatct tgccgccttc gcggtgcacc gcgtcggtga tgggcttgtg cgcccgcgcc
     1981 gccgcgctgg tggacatctt cgatgccagc ggcagcagcc agccttcgat attcggcgag
     2041 aagccgccgg tgacgatcag gcccacgccg ccgcgcgcgc gctcggcgaa gtattccgcc
     2101 agccgcggaa agttcttctt gcggtcttcc aggccggtgt gcatcgagcc catcagcacg
     2161 cggttgcgca gctgggtgaa gccgagatcg agcggcgcca gcaggcgggg atagggtgcg
     2221 ctcacggaat tcctgggtgg ttgtggaacg aatgggaaat attagccgcg ccgcactaag
     2281 atttgctgat gagagtgctg atgaatttca ccgcgaaaat gttccttacc gctgccgcgc
     2341 tgctcggcgg caccgcgttc gccggcgtgt acaagtgggt cgacgccgat ggccgcgtgc
     2401 actacggcga ccgcccgccc gccaacgcgg gcgcgcaggc ggtgagcgtg ggcgcgattc
     2461 cgtcgggcct ggaggagcgg ctgcgtgcgc tcgacccgga tttctccatc acgcgcctgt
     2521 cgggcgacct gaaggaaacg ctggtgtgcg gcgagttcga cgcgcacggc gtgcgcgacg
     2581 cacagccgcg cttcctgccg gcgctcaagg ccagcggcct ggcggaggtg ctgccggacg
     2641 aaggccagcc gacggtacgc ggccagcgcg cgccgtttta cgaccgcgac cgctgcccgc
     2701 cgccggcgcc gcagcagcgc ctggcgcagc gcagctaccg catccagttc accccggacg
     2761 ccatcgccgc ctatggcaat gcgggcagcg gccgtggatg cagatgcaat gccccaactg
     2821 cggccacgtg caggagccgg cgctgcattg cgcgggctgc ggcatcaact tcgagaaatt
     2881 ccggcagctg cagcaggggc cggcggcgcc tgcaccatcg ccgctggaaa ttacgccgca
     2941 gccgcagcgc gacaaccagc gcgcatggct gtggggcggc gcagcgctga tggggctggc
     3001 ggtggcagtc ggcgccgcga agctggtcac gctggtgcag gagcaggcgc agattcccca
     3061 tgcgctcgcg gcggtcgaga tctaccgcaa cgaaatcgca gcccggcagc gcaaccgcta
     3121 cctgctgtca tcggcgtttc ccgcggcgaa ctggcagctg ggcctgccgg cgccggatga
     3181 cgcgcactgg cgcggtttcg gaagcgtgaa cctcgtcgcc gacggccgcg tggtcttcgt
     3241 gcacgcgggc agcggcgtgc ttgacccgtc cttcaagctc gcgatttttg cgccgcccga
     3301 tacctgggcg ccgcaatgcg gcggcgtcgg cctgggtgcg cgcgcgcagc aggcgctggc
     3361 gcgctattgc gagtggaacc gcctgagcgt cgacgtgccg ccgccgccgg tgcagccgcc
     3421 agcgcccacg caggcgccgc agggcccgag cgaaaccgag cacgagcaat tctccgagcg
     3481 ctacgagcag ggcaagacgc cgctgatcga tgccgccgac cacggcgacg aagcgcagct
     3541 gcgcaagctg ctcgcggccg gcaccgaggt ggacgggcgc gaccgctacg gcagcacggc
     3601 gctgatgtat gccgcacgtg ccggctgcgc ggcctgcgtg caggcgctgc tggatgcgca
     3661 cgccgacatc catgcgcgca acaccaatgg cgcgggcgtg tggagctggg cgttgatgca
     3721 gccggcaggc gcgccatcag ccgcaacgcc ggacgagcgc ctgcatacat atgagctcct
     3781 gctgtcgcac ggcgccgacg ccaacctcga tgcgttcgat cccagtgggc gcgcgctgct
     3841 gcatcgggcg gccgacaccg acgatgcggc gctggccgcg ttcctgctcg cgcacggcgc
     3901 cgcgctcgaa ctgcgcgacg cgcagggccg cacgccgctg atgcatgccg cggtcgccga
     3961 tggcggcgat gaagtgctgg aactgttgat caaggccggc gcgcgcgcca gtgcgcgcga
     4021 caacgacaac cggccagtga tcgaactgct acggctggag tatcacggtc cacacgtgga
     4081 gcaccgcacc gcgctgttgc gcgacgcgat ggcgtacccg tcgcgccatt gagctgatga
     4141 cgtgccgcgc gaccaaaaaa tacctggacc gcacccttgg tattggtgtt agtaattgta
     4201 atatgaaggt aaaatagtta aataaatgat aacatcagga atgccggcgc ggtggagcaa
     4261 aaagcggctg ctatagtcgc cgcgcacttc ctcttccgac gagcatgcag cctgagaccc
     4321 gctatgtacg cacgccgcgc ggcgacattg cctaccaggt ggtcggcgag ggcaagcgcg
     4381 acctggtcta tctctcgggc atggtcacgc atctggatct cacctgggat ttcccggagg
     4441 ccgagcgcta cctcacgcgg ctggcgtctt tttcgcggct gatcctgttc gaccgccgtg
     4501 gcaccggcat ctccgaggcg atagcgcagg acgagcagct ctcctggagc ggctggctcg
     4561 acgacctgcg tgcggtgctc gatgcaaccg gcagcaagtc ggccgcgctg ctggccgagc
     4621 gagatgccgg cagcgtggcc atgcagttcg ccgccagtga gccggagcgc gtgtcggcgc
     4681 tgatcctggc caacaccagc gcgcgctatc tcgccgcgcc ggattacccc tgcggtgaat
     4741 cgccgcaggt ggccgagcag ctggtgcgca cggtgcatga gctgtggggc agcgagcgca
     4801 tgaccgcgct ggccatgccc agccgcgcgg acgacaccaa cttcctgcgc gtgtccgcgc
     4861 gcttccagcg ggcttcggcc acgccgagca ccgccgcgcg gcagtatcgc ttcttcttca
     4921 gccgcgatgc gcgcgaggtg ctgccgcaga tttcctgccc gacgctggtc atgcaccggc
     4981 gtgagtaccc gttcctca
//
LOCUS       DASZZC010000048         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_454437,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000048 DASZZC010000000
VERSION     DASZZC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454437"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VHE37_01210"
     CDS             complement(<1..567)
                     /locus_tag="VHE37_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVT34173.1"
                     /translation="MARAIWSGTISFGLLQVPVQLVSGEKNAEEIHFRMLDRRNRKPV
                     KYQRINAENGEEVPWGEIVKAYEYAKDEFVVLDEEDLKKAAPHNGETVEIESFVKAAD
                     IPITYYERPYFLVPPKKGAKGYVLLRETLAGSGYAGIARVVIRTRQYLAALLPFEGAL
                     LLNLMRFPHELVSPKDLDLPEGGAEEHHI"
     gene            complement(631..852)
                     /locus_tag="VHE37_01215"
     CDS             complement(631..852)
                     /locus_tag="VHE37_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34174.1"
                     /translation="MSSNRQDDTSRGSQQGSNQQQGAGQNRQNQQQQRDRPGSEGNRQ
                     QGSQGGQSGQQGSRESDQENDRGGTRQDR"
     gene            1078..1527
                     /locus_tag="VHE37_01220"
     CDS             1078..1527
                     /locus_tag="VHE37_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HVT34175.1"
                     /translation="MSNTEAIKVLNDLVETSEDGKNGFAAASERATDGQLKALFREYA
                     EDCGRALGELQESIRNLGGTPPDHGTVAGAAHRGWTKVKSMIEESNAAVLEEVERGED
                     HAKAVYAKALKAELPPLIRAMVEKQRDGVLRHHDRVRELRNQYRGKP"
     gene            complement(1531..2460)
                     /locus_tag="VHE37_01225"
     CDS             complement(1531..2460)
                     /locus_tag="VHE37_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVT34176.1"
                     /translation="MNSALRRCAIPLMLLLTACSSAPVVAPAPPPAPRPPPKIALVLG
                     GGAARGFAHIGVIKMLDALEIKPDLIVGTSAGSVAGALYAAGYDGFRLQEMAFDLDQS
                     MVADWSVFGKGLIKGQALEDYVNRAVGNKGIEQLKKPFACVATQLRTGQPILFQRGNV
                     GTAVRASSSVPGIFEPVLINGEEYVDGGLVSPVPVKYARQLGADFIIAVDVSSPPGRE
                     EADSKIAVLLKTFTIMGQSIRDAEITQADVVITPKLDQIASTDFESKHKAILAGERAA
                     LAAIPQIREKLRARMDPAPAALPPADAVPAPAH"
     gene            2741..4123
                     /locus_tag="VHE37_01230"
     CDS             2741..4123
                     /locus_tag="VHE37_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVT34177.1"
                     /translation="MSTVASTLPTSPPPLRQTPTVPGLPLLGSVLDMAKDPAKFFVDC
                     YRKYGPVFRLKVLGKPYVVIAGVEAANFMGTREGRDSLRSKEFWQGLVAEYGATRILT
                     GEDGDSHKELRDVMRRGYSRESIKGRYNELVTITDGSIARDWKPGTLVPVVQAMQYMV
                     VDQLGTILTGAAPLEYVKDIRTTILYILNVLVTRQRPTILLKHPEYKRSKARVFELGE
                     QMIATARARFGKIKDEEKNLIDDVFEAHMTNPALMPASDLIVTLTGPYVAGLDTVANT
                     TSAIAYTILKHPEVKARVLKEVDELFAPGSIDEDALLKKLPALNGAIMETMRMYPIAV
                     AQMRTATRDFVFQGHQIREGEMIYVGTSVPHFMNEYYPDADKFDIDRYAKPRAEHMTP
                     GAYSPYGRGPHTCLGKSLAEVQIALSMARLFYKLDMELDPPGYTLKTKTAPTPGPAMD
                     FKIRVKGLRH"
     gene            4163..>4765
                     /locus_tag="VHE37_01235"
     CDS             4163..>4765
                     /locus_tag="VHE37_01235"
                     /inference="COORDINATES: protein motif:HMM:NF021500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HVT34178.1"
                     /translation="MKKIIITAIAAGLLAACSRSDLPGSIDTSSGGGVTPPYLNSSLY
                     SGPAPRPGPDILYAPLADAPQLQNTGIWQAEPIRVSGASAYRKGEFLYQDFLYDDHGA
                     KQLPDPNDPRLNTLATSPSDAAIFSTPVGTYTYPANPDYANNAADLVELRVKPTADAT
                     AFRVTLNTINKPDLVAYTIALGGDATKSVAWPHGANVSSPA"
BASE COUNT          891 a         1582 c         1463 g          829 t
ORIGIN      
        1 aatatggtgc tcctccgctc ccccttccgg caggtcgaga tccttcgggc tcaccagttc
       61 atgcggaaag cgcatcaggt tgagcagcag ggcgccctcg aacggcagca gcgcggccag
      121 gtactggcgc gtacgtatca ccacgcgggc gatgccggca tagccgctgc cggccagggt
      181 ctcgcgcagc aatacgtatc ccttcgcccc cttttttggc ggcaccagga aatacggccg
      241 ttcgtagtag gtgatgggaa tatccgccgc cttgacgaat gactcgatct ccacggtctc
      301 gccgttatgc ggcgcggctt tcttaagatc ttcctcgtcc aacaccacga attcatcctt
      361 ggcatattca taggccttga cgatttcacc ccatgggact tcctcgccat tctccgcgtt
      421 gatccgctgg tacttcaccg gcttgcggtt gcgccggtcc agcatgcgga aatggatttc
      481 ttcggcattt ttctcgcccg acaccagttg taccggcacc tgcaacaggc cgaaagagat
      541 ggtgccgctc catatcgcac gcgccatggc ggtctccgat aaaaaaaggc cgccacgatg
      601 tggcggcccg gatgtacgaa tcggcagggc tcagcgatcc tggcgggtac cgccgcggtc
      661 gttttcctga tcgctttcgc ggctgccctg ctgaccgctc tggccgccct ggctgccttg
      721 ctggcgattg ccttcgctgc ccgggcggtc gcgctgctgc tgctggttct ggcggttctg
      781 gcccgcgccc tgctgctggt tcgagccctg ctgggaaccg cggctcgtgt cgtcctgacg
      841 gttgctgctc atgtctgctc tccgtgggtg gatataggtt ttgaagcccg gcgcacccca
      901 gagttccctg catcagtcgc cggaacttgc gccgcttcgc ctcgctcgaa tccacgaata
      961 cccatcaaaa aatcaatgga ggacagccat gcgtggaatc ctgttatggg taatcggcat
     1021 accactgccg atcatcatcc tgttgtacct gtttcacgtg atctgacgga gatacggatg
     1081 agcaataccg aagcgatcaa ggtgctgaac gatctggtcg agaccagcga ggacggcaag
     1141 aatggctttg cggcggcctc cgagcgcgcc actgacggcc agttgaaagc gctgttccgc
     1201 gaatacgccg aagactgcgg gcgcgccctg ggcgagttgc aggagtcgat ccgcaatctc
     1261 ggcggcacgc cacccgacca tggcacggtg gccggcgcag cgcaccgcgg gtggaccaag
     1321 gtcaagtcga tgatcgagga aagcaacgcc gcggtgctgg aagaggttga gcgcggcgag
     1381 gatcacgcca aggcggtata cgccaaggcg ctgaaggcgg agctgccgcc gttgatccgc
     1441 gccatggtgg agaagcagcg cgacggcgtg ctgcgccacc acgaccgcgt gcgtgaactg
     1501 cgcaaccagt accgcggaaa gccgtagagg tcagtgggcc ggcgccggca cagcgtcggc
     1561 aggtggtaga gctgcgggcg cgggatccat ccgcgcccgc agcttttcgc ggatctgcgg
     1621 aatggccgcg agcgcagcgc gctcccccgc gaggatggcc ttgtgcttgc tctcgaaatc
     1681 cgtggaagcg atctggtcga gcttgggcgt gatcaccaca tcagcttgcg tgatctcggc
     1741 gtcgcggatc gactgcccca tgatggtgaa ggtcttcagc agcacggcga tcttgctgtc
     1801 ggcctcttcg cgccctggcg ggctcgatac atccaccgca atgatgaaat cggcgccgag
     1861 ctgcctggcg tacttcaccg gcaccgggct gaccaggccg ccgtcgacat attcctcgcc
     1921 attgatcagc accggctcga agatgccggg cacgctggac gacgcgcgca ccgcggtgcc
     1981 gacgttgccg cgctggaaca ggatcggctg gccggtacgc agctgcgtgg ccacgcaggc
     2041 gaaaggcttc ttcaattgct cgatgccctt gttgccgaca gcgcggttga catagtcctc
     2101 cagcgcctgc cccttgatca ggcctttgcc gaacaccgac cagtccgcga ccatcgactg
     2161 gtcgagatcg aacgccattt cctgcaggcg gaagccgtcg tagcccgcgg cgtagagcgc
     2221 accggcgacg ctgccggcgc tggtgccaac gatgagatcg ggcttgatct ccagcgcgtc
     2281 gagcatcttg atcacgccga tatgcgcgaa gccgcgtgcc gcgcctccgc ccagcaccag
     2341 cgcgatcttc ggcggcggcc tgggcgcagg cggcggcgcg ggcgcaacca ccggcgcgga
     2401 cgagcaggcg gtgagcagaa gcatcagcgg gatcgcgcag cggcgcagtg cggagttcat
     2461 gtgcgtgtcg gaaaacgtgt tgagagcgaa agccggcgcg gcgattctac gcaagccttg
     2521 tggcgatgcc gtgcagatgc gttttttcct ccaccgcacc gccactttgc atgttatata
     2581 ttatatttac ttttaaattt tattatatat ttaataacac gtccaacggc agtgcggtgg
     2641 cagccagcaa cacaaaaaaa tcgcccgttt ggatgggccg cggcggcacc cgcggtgctt
     2701 taatccgcgg cagtcattca aacccgagcc gagcgcacgc atgtctaccg tcgcatccac
     2761 gctgccaact tcgccaccgc cgctgcggca gacgcccacg gtgccgggac tgccgctgct
     2821 cggcagcgtg ctcgacatgg ccaaggaccc ggcgaagttc ttcgtcgatt gctaccgcaa
     2881 gtacggcccg gtgttccgcc tgaaggtcct gggcaagccc tacgtggtca tcgccggcgt
     2941 ggaagcggcc aacttcatgg gcacgcgcga aggccgcgac agcctgcgct ccaaggaatt
     3001 ctggcagggg ctggtcgccg agtacggcgc cacgcgcatc ctgaccggag aagacggcga
     3061 cagccacaag gaactgcgcg atgtcatgcg ccgcggctat tcgcgcgaat cgatcaaggg
     3121 ccgctacaac gagctggtca ccatcaccga cggctccatc gcgcgcgact ggaaacccgg
     3181 cacgctggtg ccggtggtgc aggccatgca gtacatggtg gtcgaccagc tcggcaccat
     3241 cctcaccggc gccgcgccgc tggaatacgt gaaggacatc cgcaccacga tcctttacat
     3301 cctcaacgtg ctggtcacgc gtcagcgccc gaccatcctg ctcaagcatc ccgaatacaa
     3361 gcgcagcaag gcgcgcgtgt tcgagctggg cgaacagatg atcgccaccg ccagggcgcg
     3421 cttcggcaag atcaaggacg aggagaagaa cctgatcgac gacgtgttcg aggcgcacat
     3481 gaccaacccg gcgctgatgc cggccagcga cctcatcgtc accctcactg gcccttatgt
     3541 cgccggcctc gataccgtgg ccaacaccac ttcggccatc gcctacacca tcctcaagca
     3601 tcccgaggtg aaggcgcgcg tgctcaagga agtggacgaa ctgttcgcgc ccggcagcat
     3661 cgacgaggac gcgctgctga agaagctgcc cgcgctcaac ggcgcgatca tggaaaccat
     3721 gcgcatgtac ccgatcgccg tggcgcagat gcgcaccgcg accagggatt tcgtcttcca
     3781 gggccaccag atcagggaag gcgagatgat ctacgtcggc accagcgtgc cgcacttcat
     3841 gaacgagtat taccccgacg ccgacaagtt cgacatcgac cgctacgcca agccgcgcgc
     3901 cgagcacatg acgccgggcg cctactcgcc gtacggtcgc ggcccgcaca cctgcctggg
     3961 caagagcctg gcggaggtgc agatcgcgct gtcgatggcg cggctgttct acaagctcga
     4021 catggaactc gatccgccgg gctacacgct caagaccaag accgcgccga cgcccggccc
     4081 ggcgatggac ttcaagatcc gtgtaaaggg cctgcgccac taggcgccgc ggacttatca
     4141 tgtccgcggg aggagagcgg acatgaaaaa aataatcatc accgcaattg cggccggact
     4201 gctggccgcc tgctcgcggt cggatcttcc gggatcgatc gacacatcgt cgggtggtgg
     4261 cgtcacgccg ccgtacctga attcctcgct ctacagcggg ccggcgccgc ggcccggccc
     4321 cgacattctc tacgcgccgc tggccgatgc gccgcagctg cagaacaccg gcatctggca
     4381 ggccgagccg atccgcgtgt ccggtgcctc ggcctatcgc aagggcgaat tcctctacca
     4441 ggacttcctc tatgacgacc acggcgccaa gcagttgccc gacccgaacg atccgcgcct
     4501 gaacacgctg gccacctcgc cgtcggacgc agcgatcttc tcgacaccgg tcggcaccta
     4561 cacctacccg gccaatcccg attacgccaa caacgccgcc gacctggttg agctgcgcgt
     4621 gaagccgact gccgacgcta ccgcgttccg cgtcacgctc aacaccatca acaaaccgga
     4681 cctggttgcg tacaccatag cgctcggcgg cgatgctacg aaatccgtag catggccgca
     4741 cggagccaat gtgagcagcc ccgcg
//
LOCUS       DASZZC010000049         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_644657,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000049 DASZZC010000000
VERSION     DASZZC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644657"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="VHE37_01240"
     CDS             complement(<1..1862)
                     /locus_tag="VHE37_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HVT34179.1"
                     /translation="MTEAIDPARHAFVAASAGTGKTWLLAARVTRLLLAGARADGILA
                     LTFTRKAAGEMHERVLSRLRSMAFADDAGLRAIFAELHVPYDAPLAGRARALYEELLY
                     APLPLRAMTLHAFAQDLVSRFALQCGVPPGAEPAEDEGELVDAAWHRLQDELRAQPQA
                     APARALALLIEHGATEFTLRRIVTGFLEQRNDWRAYTQDQAAPLDFLDADLRAQLQLD
                     AQAEPWAALDAADADELLRTLHAGLVKIGGAGSIKPAPLEPALHARGQARWQALAATL
                     LKQDGKARDFKISKKLQEKLAAAELDAFIQAKDELLVRTLHARGAQLAAITHARTLAG
                     CTLGAAALAHFEDEARRRLQLPFADIEWHAGRLLREPDTAAWAAYKLDQRIDHLLLDE
                     FQDTSHAQWQLLLPALREIAAGGERARSVFVVGDAKQSIYGFRRAAPELMQQAQDWVQ
                     QRPAGWVDTLARSRRSAPAVIEFVNAVFAQDEARRLLPDFPPHATYRDDWGRVELAPL
                     IEADDAGDEPAPAFRDPPTQAAGNAENARAHAEGELIARRIRELVDARWQIDDAQGRR
                     ALGYGDIFILLQRRTHQQQLERALIAAGIPYAGAARGTLLLTVEARDLTALLRLL"
     gene            complement(1855..>3364)
                     /locus_tag="VHE37_01245"
     CDS             complement(1855..>3364)
                     /locus_tag="VHE37_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVT34180.1"
                     /translation="RRLIELDDAAPALLQPLEAPARALAPGAGARPARHWCEQLLRSL
                     QALPLWTHWPADAAGAALVDTLQQLEGALRDNPLRMTWAEFRKLLERALETATFSSAQ
                     PQSRVRLLTLEQARGLSCDALILAGAAAGSFPSAPPALVLFNHAVRAELGLGSLAQDR
                     DIALARFRGLLEAAPDVLITWAPPKPGEPAEPCAWAGLLQACGAGSAPELAHAAGSAA
                     VEVARHEELLPACAPAPRPPAPVDLLPPQMSASAHQQLVDCPYRYYAGEMLGLSQPQE
                     PDQPYSGKDFGERVHAVLQRYTQEQAGPLTAASAAQAARRLEEIGREVFARDLAARVL
                     AQAWLAQFIDLVPRLVAWMCQRSADWPQAVAEQKLRLALTPALEIHGRADRVERNARG
                     ELCVVDYKTGSTPGADDITSGEAVQAVHYALLAPQCTRIEYLPLGRDEKKLPVVVEGE
                     ALAETRSGVRTRLLQLHAQLAAGAALPANGEDHVCDFCVHAGLCRKGAWNDD"
BASE COUNT          436 a         1210 c         1239 g          479 t
ORIGIN      
        1 agcaggcgca gcagcgcggt gagatcgcgc gcctccaccg tgagcagcag ggtgccgcgc
       61 gcggcgccgg cgtagggaat gccggcggcg atcagcgcgc gctccagctg ctgctggtgc
      121 gtgcgtcgct gcagcaggat gaaaatgtcg ccgtagccga gcgcgcgccg gccctgcgca
      181 tcgtcgatct gccagcgcgc atcgaccagt tcgcggatgc gccgcgcgat cagctcgccc
      241 tcggcatggg cacgggcgtt ctccgcgttg ccggcggcct gcgtcggcgg atcgcggaac
      301 gcgggcgctg gttcatcgcc ggcgtcgtcg gcctcgatca gcggcgccag ttccacgcgg
      361 ccccagtcgt cacggtaggt cgcgtgcggc ggaaaatccg gcagcagcct gcgcgcctcg
      421 tcctgcgcga atacggcgtt gacgaactcg atcaccgccg gcgcgctgcg ccgtgagcgc
      481 gccagcgtgt ccacccagcc cgcgggtcgc tgctgcaccc agtcctgcgc ctgctgcatc
      541 agctccggcg cggcgcggcg aaatccatag atgctctgct tggcgtcgcc caccacgaac
      601 acgctgcgcg cgcgctcgcc gccggcggcg atctcgcgca gcgccggcag cagcagctgc
      661 cactgcgcgt ggctggtgtc ctggaactcg tcgagcagca ggtgatcgat gcgctggtcc
      721 agcttgtacg cggcccacgc cgcggtgtcc ggctcgcgca gcaggcggcc ggcatgccac
      781 tcgatgtcgg caaacggcag ctgcaggcgc cggcgcgcct cgtcctcgaa atgcgccagc
      841 gccgccgcgc ccagggtgca gccggccagc gtgcgtgcgt gggtgatggc ggcgagctgc
      901 gcgccgcgcg catgcagcgt gcgcacgagc agttcgtcct ttgcctggat gaacgcatcg
      961 agctcggccg ctgccagttt ctcctgcagc ttcttgctga tcttgaagtc gcgcgccttg
     1021 ccgtcctgct tgagcagcgt ggcggcgagc gcctgccagc gtgcctggcc gcgcgcgtgc
     1081 agcgccggct ccagcggtgc aggcttgatg ctgccggcgc cgccgatctt caccagcccg
     1141 gcgtgcagcg tgcgcagcag ctcgtcggca tccgccgcat cgagcgccgc ccagggctcg
     1201 gcctgcgcat cgagctgcag ctgcgcgcgc aggtcggcgt cgagaaaatc cagcggcgct
     1261 gcctggtcct gggtataggc gcgccagtcg ttgcgctgct ccaggaagcc cgtgacgatg
     1321 cggcgcaagg tgaattcggt ggcgccatgc tcgatcagca gcgccagcgc gcgcgcaggc
     1381 gcggcctgtg gctgcgcgcg cagctcgtcc tgcaggcggt gccaggcggc atcgaccagc
     1441 tcgccctcgt cttcggccgg ttcggcaccc ggcggcacgc cgcattgcag ggcgaagcgc
     1501 gacaccaggt cctgtgcgaa cgcgtgcagg gtcatggcgc gcagcggcag cggtgcgtag
     1561 agcagttcct cgtagagcgc tcgggcgcgc ccggcgagcg gcgcgtcgta tggcacatgc
     1621 agttcggcga agatggcacg cagcccggcg tcgtcggcaa aggccatgga gcgcaggcgc
     1681 gacagcacgc gctcatgcat ttctccggcg gccttgcgtg tgaaggtcag cgccagtatc
     1741 ccgtcggcgc gggcgccggc cagcagcagg cgcgtgacgc gcgcggccag cagccaggtc
     1801 ttgccggtgc cggcggaagc cgcgacgaag gcgtggcgcg cggggtcgat ggcttcagtc
     1861 atcgttccac gctcccttgc ggcacaggcc ggcgtgtacg cagaaatcgc agacgtgatc
     1921 ctcgccgttg gccggcagcg cggcgccggc ggccagttgc gcgtgcagct gcagcaggcg
     1981 cgtgcgcacg ccgctgcggg tctctgccag cgcttcgcct tcgaccacca ccggcaactt
     2041 cttctcgtcg cgcccgagcg gcaggtactc gatgcgcgtg cactgcggcg ccagcagcgc
     2101 gtagtgcacg gcctgcacgg cctcgccgct ggtgatgtcg tctgcgcccg gtgtggagcc
     2161 ggtcttgtag tcgaccacgc acagttcgcc gcgtgcgttg cgctcgacgc ggtcggcgcg
     2221 cccgtggatc tccagcgccg gcgtcagcgc cagccgcagc ttctgctcgg ctacggcctg
     2281 cggccagtcc gcgctgcgct ggcacatcca cgccaccagc cgcggcacca ggtcgatgaa
     2341 ctgggcaagc caggcttgcg cgagcacgcg cgcggccagg tcgcgtgcga acacctcgcg
     2401 gccgatctcc tcgagccgcc gtgcggcctg cgccgcgctg gccgccgtca gcggcccggc
     2461 ctgctcctgc gtgtaacgct gcagcacggc atgcacgcgc tcgccgaaat ccttgcccga
     2521 atacggctgg tcgggttcct gcggctgcga caggccgagc atctcgccgg cgtagtagcg
     2581 gtaggggcaa tcgaccagtt gctggtgcgc actggccgac atctgcggcg gcagcaggtc
     2641 gaccggcgcg ggcgggcgcg gcgcgggtgc gcacgcgggc agcaattcct cgtgccgggc
     2701 aacctcgacc gcggcgctgc cggcggcatg cgccagctcc ggcgcgctgc ccgcgccgca
     2761 ggcctgcagc agtccggccc aggcgcaggg ttccgcgggc tcgccgggct tgggcggtgc
     2821 ccaggtgatg agcacgtcgg gcgcggcttc gagcaatccg cggaatcgcg cgagcgcaat
     2881 atcgcggtcc tgcgccaggc tgcccaggcc aagctcggcg cgcaccgcgt ggttgaacag
     2941 cacgagcgcc ggcggcgcgg aggggaaact gcctgcggcg gcaccggcga ggatcagcgc
     3001 atcgcacgac aatccgcgcg cctgttccag cgtcagcagg cgcacgcgcg actgcggctg
     3061 cgcggaggag aaggtggcgg tctccagcgc gcgttcaagc agcttgcgga attcggccca
     3121 ggtcatgcgc agcgggttgt cgcgcagggc gccttccagc tgctgcagcg tgtcgaccag
     3181 cgcggcgccg gcggcatcgg ccggccagtg cgtccacagc ggcagtgcct gcaggctgcg
     3241 caacagctgt tcgcaccagt ggcgcgcagg acgtgcgccg gcgcccggcg ccagcgcgcg
     3301 cgccggcgct tccagcggct gcagcagcgc gggcgcggca tcgtccagct cgatcagccg
     3361 gcgc
//
LOCUS       DASZZC010000050         5217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_644659,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000050 DASZZC010000000
VERSION     DASZZC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644659"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VHE37_01250"
     CDS             complement(<1..1185)
                     /locus_tag="VHE37_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HVT34181.1"
                     /translation="MHLGAAAALLLAAGNTLAQTPPGEPPPPARLPAAPPIEAPAPAP
                     ASAPAGAELQFQVSAFTFQGNTVFDSATLAPLVADFLNRPVSVADLNSAADRITAWYV
                     HHGYTLALAMIPVQTINDGSVRIEVLEGRIAHLQADSHLYRPASVEHALQPLASGAIY
                     RAPDMEAGLLRLNNRPGLHARALLRPGAEAGDTNILVQTQEQRFAALASVDDYGRDSL
                     GRGRLTLDGEINNPFGLSDHAQLLALGSSTGGLYYGYAGYDSALGSDDWRGRVSYGEA
                     HYKLSGVLDGINGKSRTGGAELDWTALRRRSGQLMLTLQASRVLSNTSFEDTPLPFDE
                     RYNLVQLGLQHLWAAPGGRWATQASMDIGSSLKQPDPASADSVRLRAELDAQVRYAFA
                     ARW"
     gene            complement(1311..3047)
                     /locus_tag="VHE37_01255"
     CDS             complement(1311..3047)
                     /locus_tag="VHE37_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT34182.1"
                     /translation="MNLLPRLALCAVLVSPWVQAATPDETFAHDFGDAFLDRYWSYNT
                     DAAVQVGYYKYADRLVVPDAAWRKNYLAFLDASLKQLAAVDEKKLGPNTRADYAILKN
                     QLESQRWTLTDYRDWEWNPANYNVADPFALLQNTAYADAETRLRAISARLAAVPAYYS
                     AAKLAVNSPVREYTQLAIEQNQGALDVFGDDLEKMVAGSRLTPAERATLFDRIGAARR
                     AITDYVAFLKHVDAAQKQNTRSFRVGRKLYEQKFAYDIQTGDSAEQLYQRALAEKEKL
                     HAHMDQLADQLWPKYMGNAPKPTDRLDKIAAVIGKISEQHAAREEFVPQVKALIPQLT
                     QWISDHHLVTLDPTRPLAVRDTPPYKRGFSVASLDAPGPYDATAQTWFNVTPLDAYTP
                     EQAESFLREYNRYTMQVLAIHEALPGHYVQLLYANKSPSRIKSVFGNGAMIEGWAVYS
                     ERMMLESGWGNDAPEMWLMYSKWNLRVVCNTILDYSLHNLDLQEADAMKLLTREAFQS
                     QTEATGKWRRARLSVIQLDSYFAGFSAIYDFREQQKRELGRAFDLEKFHEQFLSYGSA
                     PVGVIKQLMTAH"
     gene            3110..3484
                     /locus_tag="VHE37_01260"
     CDS             3110..3484
                     /locus_tag="VHE37_01260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVT34183.1"
                     /translation="MKVTGRIEFEIVEHTEEKVVGRMPVQPGILNPFGVAHAGAMLWF
                     ADVCATLLAFGKNELAPGASGFPLAINLNAALLGNQKDGEFTATSAFVKRGRTLTVVR
                     TTVTGEGGRLIADVTTSHVPAR"
     gene            complement(3496..3693)
                     /locus_tag="VHE37_01265"
     CDS             complement(3496..3693)
                     /locus_tag="VHE37_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34184.1"
                     /translation="MHEQFRVLHMQCHGCGINFDRPLRWFRDHAECPFCGIDIAQAVS
                     AMLGARPAPAPSRPRQPRIMR"
     gene            4131..>5217
                     /locus_tag="VHE37_01270"
     CDS             4131..>5217
                     /locus_tag="VHE37_01270"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVT34185.1"
                     /translation="MITIHSRLLPFAALCALALSTQAQAGWDATLSRAVPLDSSIATA
                     PVPAGQRMRVLVSLKMQNADALKQYIMAAHTPGNPVYGHFLTPAQYTAGYAPSAAQAQ
                     KVVYYLRAQGFDQVTLAGNRMLVSALGTAAQVQAAFNTRLAQFSSAGKSYYANVTDAQ
                     VPDTLGGVVLAVIGVKNTFQMKTHLQQQAESHPQVPYTREYSARLKSATDAGSITMVS
                     SFPPSAWDIAYDAGDTPDGSNTTIAISTAGDDVTQVIKDLRQMEREQKLPFVPVEVRI
                     TTPLADPQDTSGDGEWSLDSQSSTAIARNVKKLIFYTVDTTVDLLSTYNEFATRADAQ
                     AGNMSYGACDITDPYVGATGGVLPELPD"
BASE COUNT          817 a         1704 c         1763 g          933 t
ORIGIN      
        1 ccagcgcgcg gcaaaggcgt agcgcacctg cgcgtcgagc tcggcgcgca ggcgcacgct
       61 gtcggccgag gctggatcgg gctgcttgag gctgctgccg atatccatgc tggcctgcgt
      121 ggcccagcgt ccgcccggcg cggcccacag gtgctgcagt cccagctgga ccaggttgta
      181 gcgctcgtcg aacggcagcg gcgtgtcttc gaagctggtg ttcgacagca cgcggctcgc
      241 ctgcagcgtg agcatcagct ggccgctgcg ccggcgcagc gcggtccagt ccagttcggc
      301 gccgccggtg cggctcttgc cgttgatgcc gtcgaggaca ccggacaatt tgtaatgcgc
      361 ttcgccatag gaaactcggc cgcgccagtc atcgctgccc agagcgctgt cataaccggc
      421 atagccgtag tacagcccgc cggtgctgga gcccagcgcc agcagctgcg cgtggtccga
      481 cagtccgaat ggattgttga tctcgccgtc cagcgtgagc cgaccgcggc cgagcgagtc
      541 gcgcccgtaa tcgtcgaccg aggccagcgc ggcgaaacgc tgctcctgcg tctgcaccag
      601 gatgttggtg tcgccggctt ccgcgcccgg ccgcagcagc gcgcgcgcgt gcaggcccgg
      661 gcggttgttc aggcgcagca ggcccgcttc catgtccggt gcgcggtaga tggcgccgct
      721 ggccagcggc tgcagcgcgt gctccacgct ggccggacga tagagatggc tgtcggcctg
      781 caggtgcgcg atgcgccctt cgagcacctc gatgcgcacg ctgccgtcgt tgatggtctg
      841 caccgggatc atggccagcg ccagcgtata gccgtggtgc acgtaccagg cggtgatgcg
      901 gtcggcggcg gagttgaggt cggccacgct gaccgggcgg ttgaggaaat ccgcgaccag
      961 cggcgccagc gtcgcgctgt cgaacaccgt gttgccctgg aaggtgaagg cgctgacctg
     1021 gaactgcagc tctgcgcccg caggcgcaga agcgggcgca ggcgccggtg cttcgatggg
     1081 cggcgccgcg ggcaggcgcg ccggcggcgg tggttcgccc ggtggcgtct gcgcaagcgt
     1141 gttgccggct gccagcagca gcgcggcggc cgcgcccaga tgcaagcgga atcttccccg
     1201 ttctgccttc atccctgttc cccaccgaaa gcgatgcttc ctggctggaa ccgagtatat
     1261 gcagcttttc ctgcgcgcgg ctgcggcgcg ctgttagctg cttcgcagaa tcagtgcgcc
     1321 gtcatcaact gcttgatcac gcccaccggc gcgctgccgt acgaaaggaa ctgttcgtgg
     1381 aatttctcca ggtcaaatgc gcgacccagt tcacgcttct gctgctcgcg gaaatcgtag
     1441 atggcggaga agccggcgaa gtagctgtcg agctggatca ccgacagccg tgcgcggcgc
     1501 cacttgccgg tggcctcggt ttgcgactgg aacgcctcgc gcgtcagcag cttcatggcg
     1561 tcggcttcct gcaggtcgag gttgtgcagg ctgtagtcga ggatggtgtt gcacaccacg
     1621 cgcaggttcc acttggaata catcagccac atctccggcg cgtcgttgcc ccagccggac
     1681 tccagcatca tgcgctcgct gtacaccgcc cagccttcga tcatggcgcc gttgccgaac
     1741 acgctcttga tgcggctggg cgacttgttg gcgtagagca gctgcacgta gtggccgggc
     1801 agcgcttcgt ggatggccag cacctgcatg gtgtagcggt tgtactcacg caggaaactt
     1861 tctgcctgct ccggggtgta cgcgtccagc ggcgtgacgt tgaaccaggt ctgcgcggtg
     1921 gcgtcgtacg ggccgggcgc gtcgaggctg gccacggaaa aaccgcgctt gtacggcggt
     1981 gtgtcgcgca ccgccagcgg ccgcgtggga tcgagcgtga ccaggtggtg atcgctgatc
     2041 cactgcgtca gctgcgggat cagcgccttg acctgcggca cgaattcctc gcgcgcggcg
     2101 tgctgctcgc tgatcttgcc gatcaccgcc gcgatcttgt ccagccggtc ggtcggcttg
     2161 ggcgcattgc ccatgtactt cggccacagc tggtcggcca gctggtccat gtgcgcgtgc
     2221 agcttttcct tttccgccag cgcgcgctgg tacagctgct ccgcgctgtc gccggtctgg
     2281 atgtcgtagg cgaatttctg ctcgtacagt ttcctgccga cgcggaacga acgggtgttc
     2341 tgcttctgcg cggcgtcgac atgcttgaga aacgcgacgt aatcggtgat tgcgcgccgc
     2401 gcggcgccga tgcggtcgaa cagcgtggcg cgctcggctg gcgtcagccg gctgccggcc
     2461 accatctttt caaggtcatc gccgaacaca tcgagcgcgc cctggttctg ctcgatggcc
     2521 agctgggtgt actcgcgcac cgggctgttc accgccagct tcgccgcgct gtagtaggcc
     2581 ggcaccgcgg ccaggcgcgc ggagatcgca cgcaggcgcg tctcggcatc ggcgtaggcg
     2641 gtgttctgca gcagcgcgaa cggatcggca acgttgtagt tggccgggtt ccattcccag
     2701 tcgcggtaat cggtcagcgt ccagcgctgc gactccagct ggtttttcag gatggcgtag
     2761 tccgcgcgcg tgttcgggcc gagtttcttc tcgtcgaccg ctgccagctg cttcagcgag
     2821 gcatcgagga aggcaagata gttcttgcgc caggccgcgt ccggcaccac caggcggtcg
     2881 gcgtacttgt agtagcccac ctgcaccgcg gcatcggtgt tgtacgacca gtagcggtcg
     2941 aggaaggcat cgccgaagtc atgcgcgaaa gtttcatccg gcgtcgccgc ctggacccat
     3001 ggagacacca gcacggcgca cagcgccagc cgcggcagca ggttcatcgg catctcctcg
     3061 cgcaattctc gtgatgtggc gctagcctag cgcaaacgcc ggagcgcaca tgaaagtcac
     3121 aggccgcatc gagttcgaga tcgtcgagca caccgaggag aaggtggtgg gccgcatgcc
     3181 ggtgcagccc ggcatcctca atccgttcgg cgtggcgcac gcgggcgcca tgctgtggtt
     3241 cgccgatgtc tgcgccacgc tgctggcctt cggcaagaac gagctggcgc cgggcgccag
     3301 cggatttccg ctggccatca acctcaacgc cgcgctgctg ggcaatcaga aggacggcga
     3361 gttcaccgcc acgtcggcct tcgtcaagcg cggcaggacg ctgaccgtgg tgcgcaccac
     3421 ggtgacgggc gagggcggca ggctgatcgc ggacgtgacc accagccacg tgccggcgcg
     3481 ctgaacggcg cgcgatcagc gcatgatgcg cggctggcgt ggacgcgacg gcgccggcgc
     3541 ggggcgtgcg cccagcattg cgctaaccgc ctgggcgatg tcgattccgc agaacggaca
     3601 ctcggcgtga tcgcggaacc agcgcagcgg gcgatcgaag ttgatgccgc agccatggca
     3661 ctgcatgtgc agtacccgga attgctcgtg caaggaagtg ctcgatgagt ttctgcgcgc
     3721 agcctactgg gcgcgcggtt gcaggagtgc gtcaggcgga tgagcgtgca tcacgggccg
     3781 ctgccgcgat cagcggggct gcgcaacgga tcaatggcgc cgcggccgtc ggaatttggc
     3841 tgaccgcccg ccggtctggt tgttattaaa tattgtttta ttttttataa aataaattat
     3901 ttataacagc cccagggccg gtgcggtgca gcgtttttcc ggctgcgccg cggctggttc
     3961 aggcctgcat gcgttccggc gccatcgccg ccgtgcgtgc gtgtcgtcgt tacctttcgc
     4021 aacgtgcgaa ccccgggatt tccctctttt ctctgttgtg ccttgcgcgc atgcttgatg
     4081 ttgatttttt gttaatgccg gccgcacggc cgccgactgg ggaaacatcc atgatcacca
     4141 ttcattcccg gctgctgccg tttgcggccc tttgcgcgct ggcgctgtcc acccaggcgc
     4201 aggccggctg ggacgccacg ctgtcgaggg ccgtgccgct cgacagttcc atcgccaccg
     4261 cgccggtgcc ggcaggccag cgcatgcgcg tgctggtcag cctgaagatg cagaacgcgg
     4321 atgcgctgaa gcaatacatc atggccgcgc acacgccggg caatccggtc tatgggcatt
     4381 tcctcacgcc ggcgcagtac accgcgggct acgcgcccag tgccgcgcag gcgcagaaag
     4441 tggtgtacta cctgcgcgcg cagggcttcg accaggtgac gctggccggc aaccgcatgc
     4501 tggtgtcggc gctgggcacg gcggcgcagg tgcaggcggc gttcaacacg cgcctggcgc
     4561 agttcagcag cgcgggcaag agctattacg ccaacgtgac cgatgcgcag gtgcccgaca
     4621 cgctgggcgg cgtggtgctg gcggtgatcg gcgtgaagaa cacctttcag atgaagacac
     4681 acctgcagca gcaggcggag agccatccgc aggttcccta tacgcgcgag tattccgcgc
     4741 ggctcaagtc cgccaccgat gccggcagca tcaccatggt gtcgtcgttc ccgccgtcgg
     4801 cctgggacat cgcctacgac gccggcgaca cgccggatgg ttccaatacc accatcgcca
     4861 tctccaccgc gggcgatgac gtgacccagg tgatcaagga cctgcgccag atggagcgcg
     4921 agcagaaact cccctttgtg ccggtggaag tgcgcatcac cacgccgctg gccgatccgc
     4981 aggacacctc cggcgatggc gaatggtcgc tcgacagcca gtcgtccacc gccatcgcgc
     5041 gcaacgtgaa gaagctgatc ttctacaccg tcgacaccac ggtggacctg ctcagcacct
     5101 acaacgaatt cgccacccgc gccgacgcgc aggccggcaa catgagctac ggcgcctgcg
     5161 acatcaccga tccttacgtc ggcgccaccg gcggcgtgct gccggaactg ccggacc
//
LOCUS       DASZZC010000051         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_580268,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000051 DASZZC010000000
VERSION     DASZZC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580268"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VHE37_01275"
     CDS             <1..675
                     /locus_tag="VHE37_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVT34186.1"
                     /translation="DNAGTDDAQRADAHLLLEILTPIAKAWPSEFALEANKLAIQVLG
                     GYGYTEDYPVERLYRDNRLNPIHEGTNGIQGLDLLGRKVTMKNGAALKLLLGRIGATA
                     KAAEADAQLQEYAQALQQAVMAAGNTTATLAGAAMKGQVDLFLANANLYLEMLGHLVL
                     AWIWLQQAQVAQRGAAEGSARDFYEGKRMACRYFFRYELPKALRQAELLCKLDDTCLA
                     MPAAAF"
     gene            694..1623
                     /locus_tag="VHE37_01280"
     CDS             694..1623
                     /locus_tag="VHE37_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT34187.1"
                     /translation="MKKAVAVLALIALGLILALASTFLPATLPAAPATDLVAPEAHPP
                     EGMKLWAIEAGTMQSQAGFAYRGGKLGEPRTFGMGGILVEHPQGRLLFDAGFGRNVDQ
                     HFRTIPWLMQAVSQYRKETPVADQLKAAGIDFMDLKGVVLTHAHWDHVSGLQDMPGVP
                     VWVPQAELDFINSGQRMTSLIRSFGAVNYHVYDFPDGAYLGFAQSYDVFDDGSVVLVP
                     APGHTPGSIIAFITLPDDQRYALLGDLVWQREGIELPAEKPWMSRNMVDNDAEGVREA
                     IVHLHQIHLAFPDMVMVPAHDRRVWDSLPQFHR"
     gene            1675..2250
                     /locus_tag="VHE37_01285"
     CDS             1675..2250
                     /locus_tag="VHE37_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HVT34188.1"
                     /translation="MENPPATDRGARYTRTAMALHWLIFLVLIGSFTIGIYMGDLHLS
                     PTKLKLVAWHKWLGVTVFFLVCLRLGWRLRHTPPALPAGMRAWEQQAAQIGHRLLYLM
                     LFVTPLTGWLMSSAKGVQTVWFTVLPLPDLLQKNPPLGKALEEVHDTLAWMFLGLIAL
                     HVAAAFKHLLLDRDEVMSHMVPGLPPPEKKS"
     gene            2247..2807
                     /locus_tag="VHE37_01290"
     CDS             2247..2807
                     /locus_tag="VHE37_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVT34189.1"
                     /translation="MKRIFLLCVAAALSFTVNAAPPSRAILAKDSTIGFVFKQENVPV
                     SGSFGKFSGTLALDPANPQAIRISIDTGSIHAGSDDADTEVVKPGWLSVAAFPQATFA
                     SKSVSNTGGANWQSTGMLTIKGIARDVTVPFTVKEHADGSAELDGQFQIKRADYKVGE
                     GEWSAFDTIANEIQVKFHLVLGAAPH"
     gene            2826..3395
                     /locus_tag="VHE37_01295"
     CDS             2826..3395
                     /locus_tag="VHE37_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVT34190.1"
                     /translation="MKKLIAAAAGLLMLGPALAAETTYTIDPAHSLQTYRYRHLGLSF
                     AQGRFNNTTGKITLDPAKKTGSADVSIDVNSLDTGVDKLDEHLKSADFFDAAKFPTIT
                     FKSTAFKFDGDRVSSVAGNLTIHGVTRPATLTVTNFACTTHPMKKVPACAANAMASIK
                     RSDFGIAGYVPNVGDEIELNLEVEALADK"
     gene            complement(3534..>4284)
                     /locus_tag="VHE37_01300"
     CDS             complement(3534..>4284)
                     /locus_tag="VHE37_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34191.1"
                     /translation="LREHEMSVQAQREEDVDSSPPGKLLLAMETRVWAELAHYALARR
                     TLARELPRGDGSAVLIVPGFGADDFATRPLRRALRELGYAAHGWGQKRNLGMRPGLRE
                     ALNLKLYSLQRDHGKVTLIGWSLGGVFVREMARHQPECVRRVITLGSPINGHPNANNM
                     LPLFKLANRGKPMKLDWEGFQRRRNPPPVPCTAIYSKSDGIVAWRCSREEELPNTENI
                     EVSGSHFGLCANPKVIRVIADRLARDSARTS"
BASE COUNT          760 a         1440 c         1401 g          683 t
ORIGIN      
        1 gataacgccg gcaccgacga cgcgcagcgc gccgacgcgc acctgctgct ggaaatcctc
       61 acgccgatcg ccaaggcctg gcccagcgag ttcgcgctgg aagccaacaa gctcgccatc
      121 caggtgctcg gcggctacgg ctacaccgag gattacccgg tggagcggct gtaccgcgac
      181 aaccgcctga acccgatcca cgagggcacc aacggcatcc agggactcga cctgctcggc
      241 cgcaaggtga cgatgaagaa cggcgcggcg ctgaagctgc tgctcggccg gatcggcgcc
      301 acggctaaag ccgccgaggc cgacgcgcag ctgcaggagt acgcgcaggc gttgcagcag
      361 gcggtgatgg cggccggcaa caccaccgcc acgctcgccg gcgcggcgat gaaagggcag
      421 gtcgacctgt tcctggccaa cgccaacctg tacctggaaa tgctcggcca cctggtgctg
      481 gcgtggatct ggctgcagca ggcgcaggtg gctcagcgtg gtgccgccga gggcagcgcc
      541 cgcgacttct acgaaggcaa gcgcatggcc tgccgctact tcttccgcta cgaactgccc
      601 aaagcattgc gccaggccga actgctctgc aagctggacg acacctgcct ggccatgccg
      661 gccgcggcgt tctgagagcg acgggagttg ccgatgaaaa aggccgtagc cgtgctggcg
      721 ctgatcgcgc tgggcctgat cctggcgctg gcatcgacct tcctgcccgc caccctgccc
      781 gccgcgcctg ctacggattt agtagcaccg gaagcacatc cgccggaagg catgaagctg
      841 tgggcgatcg aggccggcac catgcagtcg caggcgggct tcgcctaccg cggcggcaag
      901 ctgggcgagc cgcgcacctt cggcatgggc ggcatcctgg tcgagcatcc gcagggcagg
      961 ctgctgttcg atgccggctt cggtcgcaat gtcgaccagc atttcaggac catcccgtgg
     1021 ctgatgcagg cggtgtcgca gtaccgcaag gagacgccgg tcgccgatca gctcaaggcc
     1081 gccggcatcg atttcatgga cctgaaaggc gtggtgctga cgcacgcgca ctgggaccac
     1141 gtgagcggat tgcaggacat gcccggcgtg ccggtatggg tgccgcaggc cgagctggac
     1201 ttcatcaaca gcgggcagcg catgacgtcg ctgatccgca gcttcggcgc ggtgaactac
     1261 cacgtctacg attttcccga cggcgcgtac ctgggattcg cgcagagcta cgacgtgttc
     1321 gacgacggca gcgtggtgct ggtgccggcg cccgggcaca cgccgggctc gatcatcgcc
     1381 ttcatcacct tgcctgacga ccagcgctac gccttgctcg gcgacctggt gtggcagcgc
     1441 gagggcatcg aactgccggc ggaaaaaccc tggatgtcgc gcaacatggt cgacaatgac
     1501 gccgagggcg tgcgcgaagc catcgtgcac ctgcaccaga tccatctggc cttccccgac
     1561 atggtgatgg tgccggcgca cgaccgccgc gtgtgggaca gcctgccgca gttccatcgc
     1621 tgagctgcgg ctgaaaaaat tgtcgcgcgt ctacccgcgc attaggatgg ccgcatggaa
     1681 aaccctccgg caacggaccg cggcgcgcgc tacacgcgca ccgccatggc gctgcactgg
     1741 ctgatcttcc tggtgctgat cggctcgttc accatcggga tttacatggg cgacctgcat
     1801 ctgagcccga ccaagctgaa actcgtcgcc tggcacaagt ggctcggcgt caccgtgttc
     1861 ttcctggtgt gcctgcgcct gggctggcgc ctgcggcaca cgccgccggc gttgcccgcc
     1921 ggcatgcgcg cgtgggagca gcaggccgcg cagatcggcc accgcctgct gtacctgatg
     1981 ctgttcgtga caccgctgac cggctggctg atgagctcgg ccaagggcgt gcagaccgtg
     2041 tggttcaccg tgttgccgct gccggacctg ctgcaaaaga atccgccgct gggcaaggca
     2101 ctggaggaag tgcacgacac gctggcgtgg atgttcctcg gcctgatcgc gctgcatgtc
     2161 gccgccgcgt tcaagcacct gctgctggat cgcgacgagg tgatgtcgca catggtgccg
     2221 ggcctgccgc caccggagaa aaaatcatga agcggatttt cctgctgtgc gtggccgctg
     2281 cgctgagctt cacggtgaac gcggcgccgc cgtcgcgcgc catcctggcc aaggacagca
     2341 ccatcggctt cgtgttcaag caggagaacg tgccggtgtc cggcagtttc ggaaaatttt
     2401 ctggcacgct ggcgctcgat ccggccaacc cgcaggccat ccgcatcagc atcgataccg
     2461 gcagcatcca cgccggcagt gacgacgccg ataccgaagt ggtcaagccc ggctggctca
     2521 gcgtggcggc gtttccgcag gccactttcg ccagcaagag cgtgagcaac actggcggtg
     2581 ccaactggca atcgaccggc atgctcacca tcaagggcat cgcgcgcgac gtcaccgttc
     2641 cgttcacggt gaaggagcat gccgacggca gcgcggaact tgacggccag ttccagatca
     2701 aacgcgcgga ctacaaggtg ggcgagggtg agtggagtgc gttcgacacc atcgccaacg
     2761 agatccaagt gaagttccat ctggtgctgg gcgccgcccc gcactgaaaa aaccaggaga
     2821 tgaaaatgaa aaaactgatt gccgctgcag cgggcctgct gatgctgggc ccggcgctgg
     2881 ccgcggaaac cacctacacc atcgatcccg cgcacagcct gcagacctac cgctaccgcc
     2941 atctcggcct gtcgttcgcg cagggccgct tcaacaacac caccggcaag atcacgctgg
     3001 acccggcaaa gaagaccggc agcgccgacg tgtcgatcga tgtgaattcg ctggacaccg
     3061 gcgtggacaa gctcgacgag cacctgaagt cggccgattt cttcgacgcc gcgaaattcc
     3121 ccaccatcac cttcaagtcc actgcgttca agttcgatgg cgaccgcgtg agctcggtgg
     3181 cgggcaacct caccatccac ggcgtgacca ggccggcgac gctcaccgtg accaacttcg
     3241 cctgcaccac gcacccgatg aagaaggtgc cggcctgcgc ggccaacgcc atggcctcga
     3301 tcaagcgcag cgatttcggc atcgccggct acgtgcccaa cgtgggcgac gagatcgagc
     3361 tgaacctgga agtggaagcg ctggctgaca agtagcagca ccgcaacccc tctcccgccg
     3421 ggagaggggc aggggtgagg ggcggggctg gaagctcgcg caattcgcgc tgcaccgccg
     3481 tccctcaccc ggcgctgatg cgccgacctc tcccgcaggg agaggcaaga aactcaactg
     3541 gttcttgctg agtcgcgcgc caggcgatcg gcaatcacgc ggatgacttt cggattggcg
     3601 cacaggccga agtgcgagcc gctcacctcg atgttctcgg tgttgggcag ttcttcctcg
     3661 cgcgagcagc gccaggcgac gatgccgtcg ctcttgctgt agatggcggt gcacggcacc
     3721 ggcggcggat tgcgccggcg ctggaaacct tcccagtcca gcttcatcgg cttgccgcgg
     3781 ttggccagct tgaacagcgg cagcatgttg ttggcgttgg gatggccgtt gatcggcgag
     3841 cccagcgtga tcacgcggcg cacgcactcg ggctggtggc gcgccatctc gcgcacgaac
     3901 acgccaccca ggctccagcc gatcagcgtg accttgccgt ggtcacgctg cagcgagtac
     3961 agcttcaggt tgagcgcttc gcgcagcccc gggcgcatgc ccaggttgcg cttctggccc
     4021 cagccgtgcg cggcatagcc gagctcgcgc agcgcacggc gcagcgggcg cgtggcgaaa
     4081 tcgtcggcgc cgaagccggg cacgatcagc accgcgctgc cgtcgccgcg aggcagctcg
     4141 cgcgccagcg tgcgccgcgc cagcgcgtaa tgcgccagct ccgcccacac gcgcgtctcc
     4201 atcgccagca gcagcttgcc cggaggcgag gagtccacgt cttcttcgcg ttgcgcctgt
     4261 acgctcattt catgctctcg tagg
//
LOCUS       DASZZC010000052         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_903897,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000052 DASZZC010000000
VERSION     DASZZC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903897"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VHE37_01305"
     CDS             <1..841
                     /locus_tag="VHE37_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVT34192.1"
                     /translation="TLLQTRSLRVVDFHCDAQPGDAPYPEQHRDFSVSYVRKGSFGYV
                     TRGKSYELVAGSMLIGHRGDEFSCTHDHHVCGDECLSFHLSAEFADSLGGDAHTWRAG
                     GLPPLPELMVLGELAQAAATQRSGLGVDEAGMLIAARFVELASGASRPRTQAQAQARD
                     RRRAVEAALWLDEHAHEERDLDGVAAQAGLSPFHFLRLFGKVLGVTPHQYLLRARLRR
                     AARLLAEEERAVTDIALDVGFADLSNFVRTFHRAAGVSPREFRRAARGDRKIFQERLA
                     LAL"
     gene            869..1219
                     /locus_tag="VHE37_01310"
     CDS             869..1219
                     /locus_tag="VHE37_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT34193.1"
                     /translation="MYDHIGLKVQDLATSARFYKAALAPLGHVQSGDSGFGPPEAPAL
                     WLYAGTKGSSIGTHVAFRAPDRAAVDRFHREGLKAGGRDNGKPGPRADYSPTYYAAFL
                     IDPDGNNVEAVCMN"
     gene            1231..1635
                     /locus_tag="VHE37_01315"
     CDS             1231..1635
                     /locus_tag="VHE37_01315"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVT34194.1"
                     /translation="MASIRKEIIIDTPAAHAWDAVRDYGALHTRLVPGFVTACTLDGD
                     ARLVTFGNGMTSREQRVTLDDAAQRLVWTIVGGRFTHHNGALQIVADGDARCRAVWIT
                     DLLPDELAAPISAMQDQGMAIMKRTLEGSWRR"
     gene            1793..>2185
                     /locus_tag="VHE37_01320"
     CDS             1793..>2185
                     /locus_tag="VHE37_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34195.1"
                     /translation="MTSIAQLWLPILLSSLGVFFASALIHMALRWHKDDYRPLTNEDE
                     VAAAIRKGAPAPGQYMMPNCPGGPKEMQQPEMQKKFVDGPIAFLIVRQPGVPKMGPQL
                     GQWFVLTLVISAVIGHLASVSLSAPDSAH"
BASE COUNT          365 a          749 c          715 g          356 t
ORIGIN      
        1 cacgctgctg cagacgcgct cgctgcgcgt ggtggatttt cactgcgatg cgcagcccgg
       61 cgacgcgccc taccccgagc agcaccgtga cttctccgtg tcatacgtgc gcaagggcag
      121 cttcggctac gtgacgcgcg gcaagtcata cgagctggtc gccggctcga tgctgatcgg
      181 ccatcgcggc gacgagttca gttgcacgca cgaccatcac gtgtgcggcg acgaatgcct
      241 gtcgttccac ctgtccgccg aattcgccga cagcctcggc ggcgatgcgc atacctggcg
      301 cgccggcggc ctgccgccgt tgcccgagtt gatggtactg ggcgaactgg cccaggccgc
      361 ggcgacgcaa cgcagcggcc tgggcgtgga cgaggccggc atgctgatcg ccgcgcgctt
      421 cgtcgaactg gccagcggcg cttcgcgtcc gcgcacgcag gcgcaggcgc aggcgcgcga
      481 ccgccggcgc gcggtcgagg ccgcgctgtg gctggacgag cacgcgcacg aagaacgcga
      541 tctcgacggc gtggcggcgc aggccgggct gagtccgttc cacttcctgc gcctgttcgg
      601 caaggtgctg ggcgtgacgc cgcaccagta cctgctgcgc gcgcgcctgc gccgcgcggc
      661 ccggctgctg gcggaggagg aacgcgcggt gaccgacatt gcgctcgatg tcggcttcgc
      721 cgacctgtcc aacttcgtgc gcaccttcca ccgcgctgcc ggcgtgtcgc cacgcgagtt
      781 ccgccgcgcc gcgcgcggcg accgcaagat tttccaagaa cgcctcgcgc tcgcgctcta
      841 gattggcgcc tccactcacg gaggccttat gtacgatcac attggtttga aggtgcagga
      901 tctcgccacc agcgcacgct tctacaaggc agcactcgcg ccgctcgggc atgtgcagag
      961 cggcgattcc ggatttggtc cgcccgaagc gccggcgctg tggctctacg cgggcaccaa
     1021 gggcagcagc atcggcacgc acgtggcatt ccgcgcgccc gaccgcgcgg cggtcgaccg
     1081 cttccaccgt gaagggctca aggccggcgg ccgcgacaac ggcaagcccg gaccgcgcgc
     1141 cgactacagc ccgacctact acgccgcgtt tctcatcgac cccgacggca acaacgttga
     1201 agcggtgtgc atgaactgag gctcgcagac atggcatcga tcaggaagga aatcatcatc
     1261 gacacgcccg cggcgcatgc atgggacgcg gtgcgcgact acggcgcgct gcacacgcgc
     1321 ctggtgcctg gcttcgtcac cgcctgcacg ctcgacggtg acgcgcgcct ggtgacgttc
     1381 ggcaatggca tgacctcgcg cgagcagagg gtcacgctcg acgacgccgc gcagcggctg
     1441 gtgtggacca tcgtcggggg acgcttcacc catcacaacg gtgctttgca gatcgtggcc
     1501 gacggcgacg cacgctgccg cgcggtatgg atcaccgacc tgctgcccga cgaactcgct
     1561 gcgccgatca gcgcgatgca ggaccagggc atggcgatca tgaagcgcac gctggaaggc
     1621 agctggcggc gctgaaatgt cctcaccgca tcgcgctttt gtgtgttaat aattattgta
     1681 tgtttgtatt tttagacaat atttaataac acatcgaaag gcggtgcggt gggtggcatt
     1741 tgtcatgcgc gcgggactat tctcgccaca caacaacacc gggaaaccca ccatgacctc
     1801 catcgcgcaa ctgtggctgc cgatcctgct gtcgtcgctc ggcgtgttct tcgccagcgc
     1861 gctgatccac atggcgctgc gctggcacaa ggacgactac cggccgctga cgaatgagga
     1921 cgaggtggcc gcggccatcc gcaagggcgc accagcaccg ggccagtaca tgatgcccaa
     1981 ctgccccggc gggccgaagg aaatgcagca gccggagatg cagaagaaat tcgtcgacgg
     2041 gcccatcgcc ttcctcatcg tgcgccagcc cggcgtgccg aaaatgggac cgcagctcgg
     2101 ccagtggttc gtgctcacgc tggtcatctc cgctgtcatc ggccatctcg cctcggtttc
     2161 gctcagcgcg cccgacagcg cgcac
//
LOCUS       DASZZC010000053         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_904004,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000053 DASZZC010000000
VERSION     DASZZC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904004"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VHE37_01325"
     CDS             <1..490
                     /locus_tag="VHE37_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HVT34196.1"
                     /translation="PPALGNGSRFHGSRIHYSMEGWPALETGGGIHNVLPVLGDEPFL
                     VVNGDVYAECDWRSLARRGLPRGDLAHLLLVPNPPHHPKGDFGLVASRVGEDGMQYTF
                     SGISILHPDLFDGCKPGKFQLAPLLRKAAADSRVTGELFKGRWVDIGTPERLARLEKE
                     LS"
     gene            complement(510..917)
                     /locus_tag="VHE37_01330"
     CDS             complement(510..917)
                     /locus_tag="VHE37_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34197.1"
                     /translation="MSDPEVRIEPTQIAKFAAVFAEAVLTFLDSGEAQWLTWDGVSHR
                     FDFASVEMDEPQQLVLLHVDAGWEQLAGDVGEITDPEHTHYDMDALRDAIEHQLTEEG
                     VGEKILREFLRRLHRLEEDAEQDEQPQVSEDDA"
     gene            complement(910..1893)
                     /gene="lipA"
                     /locus_tag="VHE37_01335"
     CDS             complement(910..1893)
                     /gene="lipA"
                     /locus_tag="VHE37_01335"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HVT34198.1"
                     /translation="MSTPPSTIKQAAKPIVAGEKLRGADKMARIPVKIEVTAAPLRKP
                     NWIRAKNPATPEVARIKAALRANNLHTVCEEASCPNLGECFGKGTATFMIMGDICTRR
                     CPFCDVAHGRPKPLDVNEPANLARTIRDMQLRYVVITSVDRDDLPDGGAAHFASCIRE
                     SRALSPVLTIEVLVPDFRGRMDPALVIFKDTPPDVFNHNLETVPRLYQQARPGSDYEW
                     SLDLLERFKALHPRVPTKSGLMLGLGEEIAEIEQVMRDLRAHGVNMLTLGQYLQPSRH
                     HLPVVRYVEPAEFERLRVLGEQMGFDHVASGPMVRSSYHADQQAHGVLAHE"
     gene            1932..2570
                     /locus_tag="VHE37_01340"
     CDS             1932..2570
                     /locus_tag="VHE37_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT34199.1"
                     /translation="MTLKLYAFPPSPRARKPLAVLHHLKLPFELEFLDMARGAHKTPE
                     FLRLNPNGRMPVLVDDDFVLWESNAIMLHLANKVPGNFFPRDDRARADISRWLFWDMA
                     HWDSACATLLFENLVKGAFGGGAPDPVRVKEGEDKFNALAAVLDDHLSRRRWMVGDTL
                     TPADFALAAPLHYAQQCRVPVGNRPHIQRWYAQIAELDAWKKSAPPPLGSPG"
     gene            complement(2708..3475)
                     /locus_tag="VHE37_01345"
     CDS             complement(2708..3475)
                     /locus_tag="VHE37_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT34200.1"
                     /translation="MIVACYRGLRVLVHLCVGLVLALALALDWRGRLKPEKFAHWWSS
                     VLLDIFNIRLVVHGAPAAGARMTVANHVSWLDIPLIAACEPTRFVAKSEVQRWPIAGW
                     LADAGGTFYIRRGKGGSKPLLHKLTPHLARGGNVTVFPEGTTTDGREVLEFHPRLFAA
                     AIDAACAVQPVAIRYGLGARGANVAPFIGDDDLVSHILRLLREPELTAEVRYCAPLQA
                     IDLQRDELADAARTAVCAALAQPAAVPLEEVSDLLAA"
     gene            complement(3486..>4244)
                     /locus_tag="VHE37_01350"
     CDS             complement(3486..>4244)
                     /locus_tag="VHE37_01350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HVT34201.1"
                     /translation="QRFEVRFARTPEEIRETQQLRYRIFAGELGAQIDGGADGIDHDQ
                     YDRYARHLMVRDLENNTLAACTRILTGDGAANAGGFYSASEFDLAMLDALPGKLMEIG
                     RTCVAPEYRSGGAIALLWQGIAAAIIEGGYDYLFGCASIDLADGGATAHSILDSLRDK
                     YLAADAKRVRPFIPLPAADARCLHKPKMPPLLKAYLSLGAKACGEAYWDRDFNCADVF
                     MLLDVSELNPRYARHFLGQARAAASADERMPLAA"
BASE COUNT          664 a         1412 c         1463 g          705 t
ORIGIN      
        1 tccgccggcg ctgggcaacg gttcgcgttt ccacggctcg cgcatccatt actcgatgga
       61 aggctggccg gcgctggaga ccggcggcgg catccacaac gtgctgccgg tgctcggcga
      121 cgagccattc ctggtggtca acggcgatgt ctatgccgaa tgcgactggc gttcgctggc
      181 gcgccgcggc ctgccgcgcg gcgaccttgc gcacctgctg ctggtgccca acccgccgca
      241 tcatcccaag ggcgatttcg ggctggtcgc ctcgcgcgtg ggcgaagacg gcatgcaata
      301 caccttcagc ggcatctcga tcctgcaccc ggacctgttc gacggctgca agccgggcaa
      361 attccagctg gcgccgttgc tgcgcaaggc ggcagcggac agccgcgtga cgggcgaatt
      421 gttcaagggc cgctgggtgg acatcggcac gccggagcgg ctggcgcggc tggaaaaaga
      481 gctttcgtag gagcgcgcct tgcgcgcgat tacgcgtcgt cctccgagac ctgcggctgc
      541 tcgtcctgct cggcgtcttc ttccagccgg tgcaggcgcc gcaggaactc acgcaggatt
      601 ttttcgccga cgccttcctc ggtcagctgg tgctcgatgg cgtcgcgcag cgcgtccatg
      661 tcgtagtgcg tgtgctcggg gtcggtgatc tcgccgacat cgcccgccag ctgttcccag
      721 ccggcatcga catgcagcag caccagctgc tgcggctcgt ccatctccac cgaggcgaag
      781 tcgaagcggt gcgacacgcc gtcccaggtg agccactgcg cttcgccgga atcgagaaag
      841 gtgagcaccg cctcggcaaa caccgcggcg aacttggcga tctgcgtcgg ctcgatgcgg
      901 acttccgggt cactcatgcg ccagcactcc atgcgcctgc tgatcggcgt ggtacgacga
      961 gcgcaccatg ggcccggagg cgacgtgatc gaaacccatc tgctcgccca gcacgcgcag
     1021 gcgctcgaac tcggccggct ccacgtagcg caccaccggc aggtggtgcc tgctcggctg
     1081 caggtactgg cccagcgtga gcatgttgac gccgtgggcg cgcaggtcgc gcatcacctg
     1141 ctcgatctcg gcaatctcct cgcccaggcc gagcatcagg ccggacttgg tcgggaccct
     1201 ggggtgcagc gccttgaagc gttcgagcag gtccagcgac cactcgtagt ccgaacccgg
     1261 gcgcgcctgc tggtacagcc gcggcacggt ctcgaggttg tggttgaaca cgtcgggcgg
     1321 cgtgtccttg aagatcacca gcgccgggtc catgcggccg cggaaatccg gcaccagcac
     1381 ttcgatcgtc aacacgggcg acaaggcgcg cgactcacgg atgcacgagg cgaaatgcgc
     1441 ggcgccgccg tcgggcaggt cgtcgcggtc cacgctggtg atgaccacgt agcgcagctg
     1501 catgtcgcga atggtgcgcg ccaggttggc cggctcgttc acgtccagcg gcttgggccg
     1561 cccatgcgcc acgtcgcaga acgggcagcg gcgcgtgcag atgtcgccca tgatcatgaa
     1621 cgtcgcggtg cccttgccaa agcattcgcc caggttgggg caactggctt cttcacacac
     1681 ggtgtgcagg ttgttggcgc gcagcgcggc cttgatgcgc gccacttcag gcgtggccgg
     1741 atttttcgcg cggatccagt tcggcttgcg cagcggcgcg gcggtgactt cgatcttcac
     1801 cgggatgcgc gccatcttgt cggcgccgcg cagtttctcg ccggcgacga tgggcttggc
     1861 ggcctgcttg atggtagatg ggggcgtgct catggcgcta ttttaccgcg ccttccccgt
     1921 gagacctgcc catgacctta aagctctacg ccttcccgcc ctcgccgcgc gcgcgcaagc
     1981 cgctggccgt gctgcatcac ctgaagctgc cattcgaact cgagttcctc gacatggcca
     2041 ggggcgcgca caagacgccg gaattcctca ggctcaatcc caacggccgc atgccggtgc
     2101 tggtcgacga cgatttcgtg ctgtgggaat ccaacgccat catgctgcac ctggccaaca
     2161 aggtgccggg caacttcttc ccgcgcgacg accgtgcgcg cgccgacatc tcgcgctggc
     2221 tgttctggga catggcgcac tgggacagcg cctgcgccac cctgctgttc gagaacctgg
     2281 tgaagggcgc gttcggcggc ggcgcccccg accccgtgcg ggtcaaggaa ggcgaggaca
     2341 aattcaatgc gctggccgcg gtgctcgacg atcacctgtc gcgccgccgc tggatggtgg
     2401 gtgacacgct gacgccggcc gatttcgcgc tggcggcgcc gctgcactac gcgcagcaat
     2461 gcagggtgcc ggtcggcaac cgtccgcaca tccagcgctg gtatgcgcag atcgcggagc
     2521 ttgatgcgtg gaagaagtcg gcgccgccgc cactgggatc accgggatga tgttctgcgt
     2581 cgcggcacgc cagaaaacag ccgaccgcac cgcactccac catgttatta attatcgtct
     2641 gtttttaaaa ataaacaata tttaataaca tcatggatgc cggtgcggtg aagcaaaaaa
     2701 acaccgttca ggcggcgagc aggtcgctca cttcttccag cggcaccgcg gccggctgcg
     2761 ccagcgcagc acataccgcg gtgcgcgcag catccgccag ttcatcgcgc tgcaggtcga
     2821 tggcctgcag cggcgcgcag tagcgcacct cggccgtcag ttccggctcg cgcagcaggc
     2881 gcaggatatg gctgaccagg tcgtcgtcgc cgatgaaggg cgccacgttg gcgccgcgcg
     2941 cgcccagccc gtagcggatc gccaccggct gcaccgcgca ggctgcgtcg atcgccgcgg
     3001 cgaacaggcg cgggtggaac tcgagcacct cgcggccgtc ggtggtggtg ccttcgggga
     3061 acacggtgac gttgccgccg cgcgccaggt gcggcgtgag cttgtgcagc aggggcttgc
     3121 tgccgccctt gccgcggcgg atgtagaagg tgccacccgc atcggccagc cagccggcga
     3181 tgggccagcg ctgcacttcg ctcttggcca cgaagcgcgt cggttcgcac gcggcgatca
     3241 gcgggatgtc gagccaggag acatggttgg ccacggtcat gcgcgcgccg gcggcgggcg
     3301 cgccatgcac caccagccgg atgttgaaga tgtcgagcag caccgagctc caccagtgcg
     3361 cgaatttctc cggcttgagc cggccgcgcc agtcgagcgc taaggccagc gccagcacca
     3421 gccccacgca caggtgcacg agcacgcgca gcccgcggta gcaggcgacg atcatgcggc
     3481 ggatctcagg ctgccagcgg catgcgctcg tcggcggatg cggccgcgcg cgcctggccg
     3541 aggaagtggc gcgcgtagcg cggattcagc tccgacacgt cgagcagcat gaacacgtcg
     3601 gcgcagttga agtcgcggtc ccagtaggct tcgccgcagg ccttggcgcc cagcgacagg
     3661 taggccttga gcagcggggg catcttcggc ttgtgcaggc agcgcgcgtc ggcggccggc
     3721 agcgggatga acgggcgcac gcgcttggcg tcggccgcca ggtacttgtc gcgcagcgaa
     3781 tcgaggatcg agtgcgcggt ggcgccgccg tcggccaggt cgatcgaggc gcagccgaac
     3841 aagtagtcgt agccgccttc gatgatggcc gcagcgatgc cctgccacag cagcgcgatg
     3901 gcgccgccgc tgcggtactc cggcgccacg caggtgcggc cgatttccat cagcttgccc
     3961 ggcagcgcgt cgagcatggc caggtcgaat tcgctggcgc tgtagaagcc gccggcgttg
     4021 gccgcgccgt cgccggtgag gatgcgggtg caggcggcca gcgtgttgtt ttcgagatcg
     4081 cgcaccatca ggtgacgcgc gtagcggtcg tactggtcgt ggtcgatgcc gtcggcgccg
     4141 ccgtcgatct gggcgccgag ctcgccggcg aagatgcggt agcgcaactg ctgggtttca
     4201 cggatttctt cgggcgtgcg cgcgaaacgc acttcgaagc gttg
//
LOCUS       DASZZC010000054         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1097072,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000054 DASZZC010000000
VERSION     DASZZC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097072"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VHE37_01355"
     CDS             complement(<1..221)
                     /locus_tag="VHE37_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34202.1"
                     /translation="MNLLRIALALVLLGGGVRWWMQRSEHRAVLASADPYGFLSVPMP
                     SGAKPDTVLIFAPLNCPREGARRANELSR"
     gene            complement(240..1004)
                     /locus_tag="VHE37_01360"
     CDS             complement(240..1004)
                     /locus_tag="VHE37_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HVT34203.1"
                     /translation="MSTTASDATTRLAPAAPAWALTLRKLNNWLLYDLGGGVRPWKFS
                     WIINFQKGGTFLFLGALMLYYSGKTPAATSTAAWIYLALHGSYGLIWLLKDLAFPDPG
                     WQHRITIGGGLVALFALGMYWSFGWLLISGTSQPHYPLPDVAWFCLCISLCMVGSALM
                     IAADAQKYYTLRVKRGLITDGVHRYIRHPNYLGEMMIYGGFALLVWHWLPFLWLAYIW
                     GALFATNMAMKEASMARYPEWAAYKQRTWWLLPFVF"
     gene            1069..2556
                     /locus_tag="VHE37_01365"
     CDS             1069..2556
                     /locus_tag="VHE37_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVT34204.1"
                     /translation="MLSFDEYRKHDALGLAELVQRRETTASELLEVALARAQAVNGKL
                     GAIVIPMEEVARARAQTPLSGPFAGVPFLTKDLFQDYAGVPTAYGCRALKDANFTPPV
                     HAEITARWLAAGTVIFGRTNTPEFGAKGITEPDAWFPARNPWNPERTPGGSSGGSAVA
                     VAAGIVPMAGANDGGGSIRIPAAYCGLFGLKPGRGRTPWGPNFSEAMHGAAMNHVLTR
                     SVRDSAVMLDATHGPDAMSVYRLAPPERPYLLEVTRAPGKLRIGFSTRSPLGTEVDAE
                     AVTAVEDAAKLLASLGHEVEPAEPQLNGAQLCQDFLLMWFANCAATVDRVKQLTGCRN
                     GGFELDTQAMAAFGRALRANEYVESFLRWNDYASALAQFHARYDLYMTPVLAMPPPRV
                     GQLVTPAWQHVFGKILMSLGLGRLILKSGILEQTARENLKWVPYTQLANLTGAPAMSV
                     PLHWTADNLPMGVQFVAAHGGEGLLFRLAGQLEQARPWMQRVAPV"
     gene            2707..>3927
                     /gene="glnE"
                     /locus_tag="VHE37_01370"
     CDS             2707..>3927
                     /gene="glnE"
                     /locus_tag="VHE37_01370"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HVT34205.1"
                     /translation="MTDDLPQAIERACAASRFLARVRLRHADLYAPAQLGARTPGEIA
                     RTIAQAADACADEASLMQLLRLTREREMARIALRDLAGVAALDETLSDLSDLADAACA
                     AAVEFSWRKLVARHGKPCDEQGNPSRPVILGMGKLGGRELNFSSDIDLIFCFTLNGET
                     DGERGLSNEEFYARMVQDVTRYLSARTADGFVFRVDWMLRPFGSAGPPAISFAAAESY
                     YQTHGREWERYAMIKARPVAGDLAAGDELLRTLRPFVYRRYLDYNAFGALRELKGMIE
                     AEVRRKDLHDNIKLGAGGIREIEFIVQAVQLVRGGQDARLRGTQLRPLLRTLGELQLL
                     PAETAQALDHAYVFLRRAENAIQMYDDEQAHALPASAEARAALCLALGYNHWDAFSTA
                     LDAVRARVQREFERV"
BASE COUNT          671 a         1312 c         1356 g          588 t
ORIGIN      
        1 tgccgtgaca actcgttggc gcgccgcgcg ccttcgcgcg ggcagttcag cggagcaaag
       61 atcagcaccg tgtccggctt tgcgccgctg ggcatcggca ccgacaggaa tccgtatggg
      121 tccgccgatg ccagcacggc ccggtgttcc gagcgctgca tccaccagcg aaccccgccg
      181 ccgagcagga caagggcaag ggctatgcgc agcaggttca cgaagctgcg acgcgccgat
      241 cagaaaacaa acggcagcag ccaccacgtc cgctgcttgt aagccgccca ttcgggatag
      301 cgggccatgc tggcttcctt catcgccatg ttggtggcga acagcgcgcc ccagatgtag
      361 gcaagccaca ggaacggcag ccagtgccac accagcagcg cgaagccgcc gtagatcatc
      421 atctcgccca ggtaattcgg atggcggatg tagcggtgca caccgtcggt gatcagcccg
      481 cgcttcacgc gcagcgtgta gtacttctgc gcgtcggcgg cgatcatcag cgcgctgccc
      541 accatgcaca ggctgatgca caggcagaac caggcgacgt ccggcagcgg gtagtgcggt
      601 tgcgacgtgc cggagatgag cagccagccg aagctccagt acatgccgag agcaaacaga
      661 gcgaccagtc cgccaccgat ggtgatccgg tgctgccagc cgggatcggg aaaggcgaga
      721 tccttgagca gccagatcag gccataggag ccgtgcagcg cgaggtagat ccacgccgcg
      781 gtcgatgtgg ccgccggcgt tttgccgctg tagtacagca tcagcgcgcc gagaaagagg
      841 aaggtgccgc ccttctggaa attgatgatc caggaaaatt tccacggccg cacgccgccg
      901 cccaggtcgt acagcagcca gttgttgagc ttgcgcagcg tgagcgccca ggcgggcgcg
      961 gcaggagcca ggcgggtggt ggcgtccgag gcggtggtgc tcatgcggcg actccggcgg
     1021 taggcagcgg ttactctatg ccgcgctcac taaaaagggg agacggccat gttgtcgttc
     1081 gacgagtacc gcaagcacga cgcgctgggc ctggcagagc tggtgcagcg gcgcgaaacc
     1141 accgcgtcgg aactgctcga agtggcgctg gcgcgcgcgc aggcggtgaa cggcaagctc
     1201 ggcgcgatcg tcatcccgat ggaagaggtg gcacgcgcgc gggcgcagac gccgctgtcc
     1261 ggcccgttcg ccggcgtgcc tttcctgacc aaggacttgt tccaggacta tgccggggtg
     1321 ccgaccgcgt acggctgccg cgcgctcaag gacgcgaact tcacgccgcc ggtgcacgcg
     1381 gaaatcaccg cgcgctggct cgccgccggc acggtgatct tcggccgcac caacacgccg
     1441 gagttcggcg ccaagggcat caccgagccc gacgcctggt tccccgcgcg caacccgtgg
     1501 aaccccgagc gcacgccggg cggctcgtcc ggcggctcgg cggtggcggt ggcagcgggc
     1561 atcgtgccga tggccggagc caacgacggc ggcggatcga tccgcatccc cgccgcgtac
     1621 tgcgggttgt tcgggctcaa gccgggacgc ggccgcacgc cgtgggggcc gaatttttcc
     1681 gaggccatgc acggcgcggc gatgaaccat gtgctcaccc gcagcgtgcg cgacagcgcg
     1741 gtcatgctcg atgccacgca tggcccggac gcgatgtcgg tgtaccggct ggcgccgccg
     1801 gagcggccgt atctgctgga ggtgacgcgc gcgccgggca agctgcgcat cggattttcc
     1861 acgcgctcgc cgctgggcac cgaggtggat gccgaggccg tgacggcggt ggaggacgcg
     1921 gcgaagctgc tggcctcgct cggccacgag gtcgaacccg ccgagccgca gctcaacggc
     1981 gcgcagctgt gccaggattt cctgctgatg tggttcgcca actgcgcggc gacagtggac
     2041 cgcgtgaagc agctcaccgg ctgccgcaac ggcggcttcg agctcgacac gcaagcgatg
     2101 gccgccttcg gccgcgcgct gcgcgcaaat gaatatgtcg agagcttcct gcgctggaac
     2161 gactacgcat cagcgctggc gcagttccac gcgcgctacg acctgtacat gacgccggtg
     2221 ctggccatgc cgccgccgcg cgtgggccag ctggtcacgc ccgcgtggca gcacgtgttc
     2281 ggaaaaatcc tgatgtcgct cggcctcggc cggctcatcc tcaagtccgg catcctcgaa
     2341 cagaccgcgc gcgaaaacct gaaatgggtg ccgtacacgc agctggccaa cctcaccggc
     2401 gcaccggcga tgagcgtgcc gctgcactgg accgcggaca acctgcccat gggcgtgcag
     2461 ttcgtcgccg cgcatggcgg cgagggcttg ctgttccgtc tcgccggaca gctggagcag
     2521 gcgcggccgt ggatgcagcg ggtggcgccg gtttgaggcg gaaaaaggcc cgaccgcact
     2581 gccgttttga gtgttacaaa atatcgttca aatataaaaa catactataa ttaataacaa
     2641 gcaaaatgcg agtgcggtgg atgcaaaatc gcgcttcaaa tcgcgccggc gcgcacgcct
     2701 agagccatga ctgacgatct gccccaagcc atcgagcgcg cctgcgcggc cagccgcttc
     2761 ctcgcgcgcg tgcgcctgcg ccacgccgac ctgtacgcgc ccgcgcagct gggcgcgcgt
     2821 acgccgggag aaatcgcgcg caccatcgcc caggccgcgg acgcctgcgc ggacgaagct
     2881 tcgctgatgc agttgctgcg cctgacgcgc gaacgcgaga tggcgcgcat cgcgttgcgc
     2941 gatcttgccg gcgtggccgc actcgatgaa acgctgagtg atctctccga cctggccgac
     3001 gccgcctgcg ccgcggcggt ggaattttcc tggcgcaagc tcgttgcgcg ccacggcaaa
     3061 ccttgcgacg agcagggcaa cccgtcgcgc ccggtgatcc tgggcatggg caagctcggc
     3121 gggcgcgagc tgaatttttc ctcggacatc gacctgattt tctgtttcac cctgaacggc
     3181 gaaacggatg gtgagcgcgg cctgtcgaac gaggagttct acgcgcgcat ggtgcaggac
     3241 gtgacgaggt atctttcggc gcgcaccgcc gacggtttcg tcttccgcgt cgactggatg
     3301 ctgcgcccgt tcggctcggc cggcccgccg gcgatcagct tcgccgcggc cgagagctac
     3361 taccagacgc atggccgcga gtgggaacgc tacgccatga tcaaggcgcg gccggtggcg
     3421 ggtgatctgg ctgccggcga cgaactgctg cgcacgctgc gcccgttcgt ctatcgccgc
     3481 tacctcgact acaacgcctt cggcgccctg cgcgagctca agggcatgat cgaggccgag
     3541 gtgcggcgca aggacctgca cgacaacatc aagctcggcg ccggcggcat ccgcgaaatc
     3601 gagttcatcg tgcaggcggt gcagctggtg cgcggcggcc aggacgcgcg cctgcgcggc
     3661 acgcagctgc gcccgctgct gcgcacgctg ggcgagctgc agctgctgcc ggcggaaacc
     3721 gcgcaggcgc tggatcacgc ctacgtgttc ctgcgccgcg ccgagaacgc catccagatg
     3781 tacgacgacg agcaggcgca tgcgctgccg gcgtcggccg aagcgcgcgc ggcgctgtgc
     3841 ctcgcgctgg gctacaacca ctgggatgcc ttcagcaccg cgctcgacgc ggtgcgcgcg
     3901 cgcgtgcagc gcgagttcga gcgcgtg
//
LOCUS       DASZZC010000055         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1032683,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000055 DASZZC010000000
VERSION     DASZZC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032683"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1014)
                     /locus_tag="VHE37_01375"
     CDS             complement(10..1014)
                     /locus_tag="VHE37_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34206.1"
                     /translation="MRPQPIERQRRALPTTSERPVPVALKAVTISLRTRIVVWLMRHF
                     LRPLLRWVVHGSLDRIARVQMFLAARSCRHTAGLPLDYVVLGRVPGHFVGKLTDTHKT
                     AILYLHGGAFLIPAVPETHVTMMALLCRDLDAVGFMADYRLAPWNRFPAALDDCERAY
                     EALLELGFAPERIVIAGESAGGNLTLGVLQRIRRKKLPMPACAVPISPATELGRIHGP
                     PSRALKYKSDPILPMDSLARVAEMYAGGMDASDPELSPLYADLRGFPPLYLLASDAEV
                     LLDDTTLLARRAREQGVNVRYDIWPILPHAFPLFEAVMPEVKQARTDIVAFIKEHVRV
                     "
     gene            1509..4088
                     /locus_tag="VHE37_01380"
     CDS             1509..4088
                     /locus_tag="VHE37_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34207.1"
                     /translation="MRTTRIHRASLALLVAGPVSISWAQDTPPGPAAAAPADVATIPV
                     AQIPDAAPAPTPEADGATRLAPVYVTAQKRKQSLSEVPITISAFNGEQLAQLGVNDTR
                     DLGLVVPGLAVNDSGQGTPIYTLRGVGYNDTTYTATGTVGLYLDEVDLPYSVMSKGLA
                     VDIERVEILKGPQGTLYGRNTTGGLIDQIANKPTENLQTGLKVGYGRFKTVDGEGYVS
                     GPLADTVRGRIAVRALNEDQGWQYSNTRPDDHLGRMQKGSARAALEWQPAESFQLRLV
                     GDGWVDRSQPQAPQAVGINPQNGILGSAALSPDVKSYPLIPQDGASNQVADWSPDRAW
                     RRNDRFESETLRADWRPGDTTQWITQLSHLRVEANPSDDIQSGFNFYNLESSTTAFIN
                     TTSVESRFSGQFGDALHWMFGGNVSHDKASEDHYVLIDTESAFFPIEPAALTGVLQGL
                     AADGLDFGQTAQSIPGVPAQYSGIVESVQNGGSPLTNRFHSQGRVDNREYAAFLNLDW
                     QFVRTLTLNLGARETINNQDFHGCSREAPESVGLGASNLSTFLSFLDAAQYTLQTGQP
                     GNPSVAMKGDCFSVGADGNHNEFHGTLDERNVSGRVALSWQPAPEYLAYASVSRGYKA
                     GGFPVLNACCQYQFAPTRQERLDAAETGAKLTLLDRRVQINLAAFYYDYTDKQLLTKV
                     RDPVFGPLPVLRNAPRSHVYGGEIELQSRPLNGLYLSVAAARIVTQIDEFVSTNDQGN
                     TQDFAGRPFNFSPPYEINGVADYTATLGSWEAGLGVNVQRSGDTNGTLAQTPAYDMPA
                     YTLLGAQLHLATPTRRWTARVYGYNLTNAFYSEASFNIGDSIARFAGFPRTYGIALAY
                     NLQ"
     gene            complement(4124..>4377)
                     /locus_tag="VHE37_01385"
     CDS             complement(4124..>4377)
                     /locus_tag="VHE37_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34208.1"
                     /translation="AADEVGEVLRNGHVQCSFVVILIILSTPGVVEAACLPLPPGEVA
                     AQRRVREHGRKVDLVYAEPTPLIWRFAPPSPDGRREAIN"
BASE COUNT          797 a         1471 c         1429 g          680 t
ORIGIN      
        1 ccccgctcct tacacgcgca cgtgttcctt gatgaacgcc acgatatcgg tccgcgcctg
       61 tttcacttcc ggcatcaccg cctcgaacag cgggaaggcg tgcggcagga tcggccagat
      121 gtcgtagcgt acgttcacgc cctgttccct ggcgcggcgc gccagcaacg tggtgtcgtc
      181 gagcagcacc tcggcgtcgc tggccagcag gtacagcggc gggaacccgc gcaggtcggc
      241 gtacagcggc gacagttccg ggtccgacgc gtccatgccg ccggcgtaca tctcggccac
      301 gcgcgccagc gagtccatcg gcaggatcgg gtcgctcttg tacttgagcg cgcgcgacgg
      361 cggcccgtga atgcggccga gttcggtggc cggcgaaatc gggacggcgc aggccggcat
      421 cggcagcttt ttcctgcgga tgcgctgcag cacgcccagc gtgagattgc cgccggccga
      481 ttcgccggcg atgacgatgc gttccggcgc aaagcccagc tcgagcagcg cctcgtaggc
      541 gcgctcgcaa tcgtccagag cggcaggaaa gcggttccac ggcgccagcc ggtaatcggc
      601 catgaagccc accgcgtcga gatcgcggca caacagcgcc atcatcgtca catgcgtttc
      661 cggcaccgcc gggatcagga acgcgccgcc gtgcaggtaa aggatcgcgg tcttgtgcgt
      721 gtcggtcagc ttgccgacga aatgcccggg cacgcgcccg agcaccacgt agtcaagcgg
      781 cagccccgcc gtgtgcctgc agctgcgcgc cgcgaggaac atctgcacgc gcgcaatgcg
      841 gtcgagcgag ccatgcacca cccagcgcag cagcggccgc aggaagtggc gcatcagcca
      901 caccacgatg cgcgtgcgca gggagatggt caccgccttg agcgcgaccg gcacgggccg
      961 ttcgctggtg gtgggcagcg cgcggcgctg tcgctcgatc ggttgcggtc gcatcgtcag
     1021 ggctcggagt gggcgaaaaa ccaccccacc gcaccggccg ttacactgtt attaattatc
     1081 tagtgatttt attttcacat gatattttat aacagcccgg accgtggtgc ggtgtaggct
     1141 ttgcggcagt ctaaatcggg cgcgggcgac cgcgtcgtcc attcatgcga gtagcgcaac
     1201 gaccggatcg aaaagtctga ccgcgctgcg caacgacggc ctgcgaggcg ccacaatctc
     1261 tccaggcctg tgccggccag agctcgcatc aggcctgcag cccgcggcgc ggacagtgcg
     1321 gtagcaacag ccggaacgcg cggcgcgggc gacatgccgc cggatgggcc tcaatggcag
     1381 tgacgcgtgg gctgggccca gcttgcattg gacgccgcgc gcccttccgg ttcctctcgc
     1441 aacagcgcat atgaaaattc catctcgtca ttggacgctg tgccgctgct gaccaatagt
     1501 gcggggccat gcgaacaaca agaatccacc gcgcgtccct ggcgctgctg gtcgccgggc
     1561 cagtctccat ttcgtgggca caggacactc cgcctggtcc agcggcggcc gcgcctgcgg
     1621 acgttgcgac catcccggtt gcgcaaatcc cggatgcggc gcccgcaccc acaccggaag
     1681 ccgacggcgc cacgcgcctc gccccggtct acgtcaccgc gcagaagcgc aagcagagcc
     1741 tgtcggaagt gccgatcacg atcagcgcgt tcaacggcga gcagttggcg caactcggcg
     1801 tcaacgacac gcgcgatctc ggcctggtgg tgcccggcct cgcggtcaac gacagcggcc
     1861 agggcacgcc gatctacacg ttgcgcgggg tcggttacaa cgacaccacg tacacggcga
     1921 ccggcaccgt cggcctgtat ctcgacgagg tggacctgcc gtactcggtg atgagcaagg
     1981 gcctggcggt cgatatcgag cgcgtggaaa tcctcaaggg cccgcagggc acgctctatg
     2041 gccgcaatac gactggcggc ctgatcgacc agatcgcaaa caagccgacg gaaaatttac
     2101 agaccgggct caaggttggc tacggccgct tcaagacggt cgacggcgag ggctacgtca
     2161 gcgggccgct tgccgacacg gtgcgcgggc gcattgcggt gcgcgcgctc aacgaagacc
     2221 agggctggca gtacagcaac acgcggccgg acgatcatct cggccgcatg cagaaaggct
     2281 cggcgcgcgc ggcgctggag tggcagccgg cggagtcgtt ccagctgcgc ctggtcggcg
     2341 acggctgggt cgaccgcagc cagccgcagg cgccgcaggc agtcggcatc aatccgcaga
     2401 acggcatcct cggcagtgcg gcgctgtcgc ccgacgtgaa gagctacccg ctgatcccgc
     2461 aggacggcgc gtcgaaccag gtggcggact ggagcccgga ccgcgcctgg cggcgcaatg
     2521 accgcttcga gagcgaaacc ttgcgtgccg actggaggcc cggcgacacc acgcagtgga
     2581 tcacgcagct gtcgcacctg cgcgtcgagg ccaatccgtc ggacgacatc cagtcgggct
     2641 tcaacttcta caacctggaa tcgagcacca cggcgttcat caacaccacg tcggtggaaa
     2701 gccggttctc cgggcagttc ggtgacgcgc tgcactggat gttcggcggc aacgtcagcc
     2761 acgacaaggc cagcgaagac cactacgtgc tgatcgacac cgagagcgcg ttcttcccga
     2821 tcgagccggc ggcgctgacc ggcgtgctgc aggggctggc cgcggacgga ctggatttcg
     2881 gccagaccgc gcagtcgatt cccggcgtgc cggcgcaata ttccggcatc gtcgagagcg
     2941 tgcagaacgg cggcagcccg ctgaccaacc gcttccactc gcagggccgc gtggacaacc
     3001 gcgagtacgc ggcgttcctc aacctcgact ggcaattcgt gcgcacgctc acgctcaacc
     3061 tgggcgcgcg cgagacgatc aacaaccagg acttccacgg ctgctcgcgc gaggcgccgg
     3121 agagcgtggg cctgggcgcg tcgaacctgt cgaccttcct cagctttctc gacgccgcgc
     3181 agtacaccct gcagaccggc cagccgggca atccttcagt ggcgatgaag ggggattgct
     3241 tcagcgtcgg cgcagacggc aatcacaacg agttccacgg cacgctggac gagcgcaacg
     3301 tgtccggccg cgtggcgctg tcgtggcagc cggcgccgga atatctcgcc tatgcgtcgg
     3361 tgagccgcgg ctacaaggcc ggtggcttcc cggtgctcaa cgcctgctgc cagtaccagt
     3421 ttgcgcccac gcggcaggag cggctggacg ccgccgagac cggcgcaaaa ctgacgctgc
     3481 tcgatcgccg cgtgcagatc aaccttgccg cgttctacta cgactacacc gacaagcagc
     3541 tgctgaccaa ggtacgtgac ccggtgttcg gcccgctgcc ggtgctgcgc aacgcgccgc
     3601 gctcgcatgt ctacggcggg gaaatcgaac tgcagtcgcg gccgctcaac gggctgtatc
     3661 tctcggtggc ggcggcgcgc atcgtcaccc agatcgacga gttcgtcagc accaacgacc
     3721 agggcaacac gcaggatttc gccgggcggc cattcaattt ctcgccgccg tacgaaatca
     3781 acggcgtggc cgactacacc gcgacgctgg gctcgtggga ggcggggctg ggcgtcaacg
     3841 tgcagcgcag cggcgacacc aacggcacgc tggcgcagac gccggcgtac gacatgccgg
     3901 cttacacgct gctcggcgcg cagttgcacc tggcaacgcc gacgcggcgc tggaccgcgc
     3961 gcgtgtacgg ctacaacctg accaacgcct tttatagcga agccagcttc aatatcggcg
     4021 acagcattgc gcgcttcgcc ggctttccgc gcacttatgg aatagcgctg gcgtataacc
     4081 tccagtgatt cttgattcat caaaaagatc tgatgaagat tgattaattg attgcttccc
     4141 ttctcccatc gggagaaggt ggcgcgaagc gccagatgag gggcgtcggt tctgcgtaca
     4201 ccaggtccac tttccggccg tgttccctca cccgccgctg cgcggcgacc tctcccggcg
     4261 ggagaggcaa gcaggctgct tcgaccacac ccggagtcga aagaatgatc agaatgacaa
     4321 cgaaggagca ctgcacatga ccgttgcgca atacttcccc aacctcatcg gcggcat
//
LOCUS       DASZZC010000056         9986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_645046,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000056 DASZZC010000000
VERSION     DASZZC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645046"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VHE37_01390"
     CDS             complement(<1..1156)
                     /locus_tag="VHE37_01390"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HVT34209.1"
                     /translation="MGKAPEKPPTMPPDTLPPPRRRGFRYHGPRVLAFVVVLIALLVL
                     VWDWNWFRPLVERKASAAIGRRVTLSHFDVNLGWHPLISADGITVANPDEFDLAGNMA
                     QADRIAVRLDLRELFHRRLKLDELILDHPDISLARTPSGHVNWTFTFPGKQGETPDNA
                     AGPEIAALTISDGRLHLADPKELKADVNATFETRNDPAGGEPQLVAQAQGRYNGQPVT
                     ASFTGGSVLTLRDPAHPYPVDLKITNGNTHVSADGRVLQPLTFGGADVTLQLSGDDLA
                     DLYPLTGVPLPHTAKYQLRGALDYAAGSIRFNHFKGSVGESDLSGDVAVTPGRGRRRE
                     VNATLHSEKVVLADLGGFIGATPGNSEAPTQTAKTRAEHAQNAASGKLLPD"
     gene            1255..3387
                     /locus_tag="VHE37_01395"
     CDS             1255..3387
                     /locus_tag="VHE37_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT34210.1"
                     /translation="MFAPRRLSQLLPAPAAADRVGRPLERERLLSTLLSNLDGMVYRC
                     RHDQHWTMEFVSEGCTDLTGYRPEDLVGNQRISYELLTHPEDRAPVRRTIEHALQQRT
                     RYELEYRIVRADGATRWVWERGAGSFTADGTLAALEGIVQDISGRRQAEQSLREAERR
                     YRSIFENAIEGIFQSTPADGYITVNPALARMYGYDSPEQLIAQLRDIDHQLYVEPNRR
                     ADFIAAMERDGSVTNFESRVYRRDGTIIWISENARAVRDEAGVLLFYEGTVETITERK
                     LHDSAMRHQATHDALTGLPNRSLLHEKLAHALEAAAHNGSLVAVAFIDLDQFKIVNDS
                     LGHHMGDELLKTMAQRLQACLREHDVVARQGGDEFVLMVTGALSEAEIGRIARRLIAE
                     VSRPCMIAGREVSVTCSIGITLSPADGSDIEALLRNADAAMYRAKELGRNNYQYFAAE
                     MNARVAGRLEMLTRLRRALEREEFELYYEPRYDFDARRIVGVEALIRWHQDGAVVRPA
                     EFIPLAEDTGLIVPIGDWVLRQACAQTRRWHDAGLRTVPVSVNLSRRQLSQGDIAAQV
                     AQVLAASGLQPQWLELEITESMLMQDTERAIALLSRLREKGVQISMDDFGTGYSSLSY
                     LKKLPVQRLKIDQAFLRDLPDDADARAMVKAIISLAHNLGLKVVAEGVETAAQFEFLR
                     GADCDEVQGWYLARAMPAARFAELLQEV"
     gene            complement(3384..4934)
                     /locus_tag="VHE37_01400"
     CDS             complement(3384..4934)
                     /locus_tag="VHE37_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVT34211.1"
                     /translation="MTDPAKAEPRTTEAMLRAMVGCASDIIAMIDARGVILHINQAVQ
                     AIGGYAPAELVGTRLLDLVHPDDLPEVIASISRLQDEHALVIRAQRRYRHKLGHWITL
                     ESSARHANHGCGVDGIVVTMRDISERKRAELALARVTRAYRTLSAGSSALVEAGTELA
                     LLQSMCAAIVDPGGYRMAWVGMARHDAGRTVQPIAWSGEVENYLRQIRISWADDEHGR
                     GPTGRAVREGTPQVAEDIAHDPSMLPWRKLALQLGFASSLALPLCHGGETYGALMIYA
                     PEPDAFAGDEVALLGDLAQKLAYGIAALRTRAQHESALQRLERSMVAAVQAIAATLEQ
                     RDPFTAGHQRRTADLSSAIGAAMGMSSERLRGLELAAAVHDVGKIMIPAEILSKPGRL
                     SDLEMQMVRLHPLAGHDILKDIDFPWPIAEIVYQHHERMDGSGYPRGLKGDAILLEAR
                     IIAVADTVVAMSTHRPYSSAQGLDSALVELASCSGKTLDAAVVDVCLSLFHNRRFHLH
                     HDELPARR"
     gene            5108..5668
                     /locus_tag="VHE37_01405"
     CDS             5108..5668
                     /locus_tag="VHE37_01405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVT34212.1"
                     /translation="MLRIAIVVGSTRPGRKAEAVAQWVHGLASRRNDAQYELLDIAQY
                     PLPLLDEPVPPSMHQYSKDHTRAWAAAVAQYDGYVFVTPEYNHGPSAALKNALDFVYA
                     EWNNKAAGFVGYGSAGGVRAVEQLRQVMGELQVADVRAQVSLSLYTDFENMSAFKPAS
                     MHEKSVNTMLDQLLAWSGALKTLRAR"
     gene            5844..6074
                     /locus_tag="VHE37_01410"
     CDS             5844..6074
                     /locus_tag="VHE37_01410"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HVT34213.1"
                     /translation="MSHDRARRGHSERVSLARRHEIRDWSRELECTPAELRAAVRAVG
                     RSVGAVRLYLARARAARASRRVVYRGGAAAHS"
     gene            complement(6075..6533)
                     /locus_tag="VHE37_01415"
     CDS             complement(6075..6533)
                     /locus_tag="VHE37_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HVT34214.1"
                     /translation="MSPAPVATQDAATPRPQPEPPNYITERGMAKLQERLGRARRRLT
                     ALGMTELERDFAAREVQQLETRVASAVPVARQARDDIGFGATVDVSSGGQTRRWRLVG
                     ADEADPPRGAIHWNSPLARALLGARVGDAVTFNAPAGAVELVVLDVSYAA"
     gene            complement(6558..7223)
                     /locus_tag="VHE37_01420"
     CDS             complement(6558..7223)
                     /locus_tag="VHE37_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34215.1"
                     /translation="MPGMAPNSKRASRYHGSAQDELPRELEFEAGDERSGRAGDLRSP
                     ADVAEEFPPERERLAGRTGGELPHPGITADDLAPETLLDDNRSREPAARELRDAVDST
                     LRTVDESAIGEGAGPDEAEMADIEPVAPAEVAQLKRKAKVARASAARASKKPAPPRKA
                     PTQRAAGKQERRKRPAKKTAKRAAKKSAAKSAAGRGKAAVRRKPATAGSRARATGQKR
                     RSR"
     gene            7686..8483
                     /locus_tag="VHE37_01425"
     CDS             7686..8483
                     /locus_tag="VHE37_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVT34216.1"
                     /translation="MSPTAAAAVAEVPATSAALPLRLLTLNLHKGFSTSGRRFVLHEL
                     RDAIRGISADIVCLQEVLGEHRRHGARQGARWPKVPQYEFLADSIWHQHAYGRNAVYD
                     GGHHGNAVLSKLPIVRFQNHDISIGGPERRGILHCVLQPQGRAEIHVLCTHLGLMEQH
                     RQQQIDRLGALIDNAVPADAPLLLAGDFNDWRLRGHARLQRELQLREVFVEAQGQCAR
                     TFPARWPLMRLDRIYYRHARVMCPSVLSARPWSHLSDHVALTTELEL"
     gene            8480..9706
                     /gene="clsB"
                     /locus_tag="VHE37_01430"
     CDS             8480..9706
                     /gene="clsB"
                     /locus_tag="VHE37_01430"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HVT34217.1"
                     /translation="MSGWRWHSGNRVRLLENGEEYFPRVFEAIGQAQREVIIETFILF
                     EDGVGRRLEEAVIAAARRGVRVDITVDGYGSADLSPRFAAALAQAGVRLHVFEPIVRL
                     LGWRTGLFRRLHRKIVVIDGALAFVGGINFAHDHLAEAGEESKQDYAVEVEGPVVADI
                     YRLVAPGRAPRPLPWWRRWLLAHSRAPIEAPVVGAVEAMLAQRDNHHHRTDIERAYRL
                     GVRLARRSVIIANAYFFPGYRLLRDLRNAARRGVRVQLIMQGKPDMPWVRWLSTSFYG
                     YLARGGVEIYEYCQRPIHAKVAVIDDEWATVGSSNLDPLSLSLNLEANLVLRNREFSL
                     QLRQRLERLMSESCTRLEQAQFRRRGPFWQLVTVLAFHAARRFPAVAGWLPAHRPVRT
                     MLTGEGLAVPTQRKAA"
     gene            9760..>9986
                     /locus_tag="VHE37_01435"
     CDS             9760..>9986
                     /locus_tag="VHE37_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HVT34218.1"
                     /translation="MKRMQSGPVALCLVLAVAGAYAGSSTDPTKASGPTTVKEDDLEF
                     MTKASQAGMAEVQEAQVASTKAMDAGVRSFA"
BASE COUNT         1468 a         3390 c         3545 g         1583 t
ORIGIN      
        1 tgtccggcag cagctttccc gatgccgcgt tctgcgcatg ctcggcacgc gtcttcgcgg
       61 tctgcgtcgg cgcttccgag ttgccgggcg tggcgccgat gaagccgccg agatcggcca
      121 gcaccacttt ttccgaatgc agcgtggcgt tcacctcgcg ccgcctgccg cgcccgggcg
      181 tgacggcgac atctcccgac aggtcgctct cgccgacgct gcccttgaaa tggttgaagc
      241 ggatgctgcc ggcggcgtag tcgagcgcgc cgcgcagctg gtacttcgcc gtgtgcggca
      301 gcggcacgcc ggtgagcggg tagagatccg ccaggtcgtc gccggacagc tgcagcgtca
      361 cgtcggcgcc gccgaaggtc agcggctgca gcacgcggcc atccgcgctc acgtgcgtgt
      421 tgccgttggt gatcttcagg tcgacgggat acggatgcgc gggatcgcgc agcgtcagca
      481 ccgagccgcc ggtaaagctg gcggtgaccg gctggccgtt gtagcggccc tgtgcctgtg
      541 ccaccagctg cggctcgccg ccggccgggt cgttgcgcgt ctcgaaggtg gcgttgacgt
      601 cggccttgag ttccttcgga tcggccaggt gcaggcggcc gtcgctgatg gtcagcgccg
      661 ctatttccgg cccggcggcg ttgtccggcg tttcgccctg cttgccggga aaggtgaagg
      721 tccagttcac gtggccggat ggcgtgcgtg cgaggctgat gtccgggtga tcgaggatca
      781 gttcatcgag cttcagcctg cggtgaaaca gctcgcgcag gtccagccgc acggcaatgc
      841 ggtcggcctg cgccatgttg ccggcaagat cgaactcgtc ggggttggcc acggtgatgc
      901 cgtcggcgga gatcagcgga tgccagccca ggttcacgtc gaagtggctg agcgtgaccc
      961 tgcgcccgat ggcagcggag gccttgcgtt cgaccaatgg ccggaaccag ttccagtccc
     1021 agaccagcac cagcaaggcg atgagcacga cgacgaacgc aagcacgcgc gggccgtggt
     1081 aacggaagcc gcggcggcgc ggcggcggga gcgtgtctgg cggcatggtc gggggtttct
     1141 cgggtgcttt gcccattgga gtccgcgccc gcgcagaggt tgcaccagcg tggcgcgcgg
     1201 cccgtggcag gaatcttgct gaaccggggt catgactttt ccgctgcgag cgccatgttc
     1261 gcgcccaggc gcttgagcca gctgttgccc gcgccagccg ccgccgaccg tgtcggccgg
     1321 ccgctggagc gcgagcgcct gctgtccacg ctgctgtcga atctcgacgg catggtctac
     1381 cgctgccggc atgaccagca ctggaccatg gaattcgtca gcgaaggctg caccgatctc
     1441 accggctacc gccccgagga cctggtcggc aaccagcgca tttcctacga gctgctcacg
     1501 cacccggagg accgcgcgcc cgtgcgcagg accatcgagc acgcgctgca acagcgcacg
     1561 cgctacgagc tggaataccg catcgtgcgc gccgatggtg caacccgctg ggtatgggag
     1621 cgcggcgccg gcagcttcac cgccgacggc acgctggccg cgctggaagg catcgtccag
     1681 gacatcagcg gacggcgcca ggccgagcag tcgctgcgcg aggcggagcg gcgctaccgc
     1741 agcatcttcg agaacgccat cgagggcatc ttccagtcga cgccggccga cggctacatc
     1801 acggtcaatc ctgccctggc caggatgtat ggctacgact cgcccgagca gctgattgcg
     1861 cagctgcgcg acatcgacca ccagctctac gtcgagccca accgccgtgc cgatttcatc
     1921 gcggcgatgg agcgcgacgg ctcggtcacc aacttcgaat cccgcgtgta ccggcgcgac
     1981 ggcaccatca tctggatctc cgaaaacgcg cgtgcggtgc gcgacgaggc cggcgtgctg
     2041 ctgttctacg agggcacggt ggagaccatc accgagcgca agctgcacga ctcggccatg
     2101 cgccaccagg ccacgcacga cgcgctcacc gggctgccca accgttcgct gctgcacgag
     2161 aagctggcgc atgcgctgga agcggctgcc cataacggca gcctggtggc ggtggccttc
     2221 atcgacctgg accagttcaa gatcgtcaac gacagcctcg gccaccacat gggcgacgag
     2281 ctgctcaaga ccatggcgca gcgcctgcag gcgtgcctgc gcgagcacga cgtggtggcg
     2341 cgccagggcg gcgacgagtt cgtgctgatg gtcaccggcg cgctgagcga agcggagatc
     2401 ggccgcatcg cccggcgcct gatcgccgag gtgtcgcggc cgtgcatgat cgccgggcgc
     2461 gaggtgtcgg tgacctgctc catcggcatc acgctgtcgc cggccgacgg cagcgatatc
     2521 gaggcgctgc tgcgcaacgc cgatgccgcg atgtaccgcg ccaaggaact cggccgcaac
     2581 aactaccagt acttcgccgc cgagatgaac gcgcgcgtgg ccgggcggct ggagatgctc
     2641 acgcgcctgc gccgcgcgct ggagcgcgag gagttcgagc tgtactacga gccgcgctac
     2701 gacttcgacg cgcgccgcat cgtcggcgtc gaggcgctga tccgctggca ccaggacggc
     2761 gccgtggtgc gcccggccga gttcatcccg ctggccgagg acaccggcct gatcgtgccg
     2821 atcggcgact gggtgctgcg ccaggcctgt gcgcagacgc ggcgctggca cgacgcgggc
     2881 ctgcgcacgg tgccggtgtc ggtgaatctc tcgcgccggc agctgtcgca gggtgacatc
     2941 gccgcacagg tggcgcaggt gctcgcggcc agcgggctgc agccgcaatg gctggaattg
     3001 gagatcaccg agagcatgct gatgcaggac accgagcgtg ccatcgcgct gctgagccgg
     3061 ctgcgcgaaa agggcgtgca gatctcgatg gacgatttcg gcaccggcta ctccagcctg
     3121 tcgtacctga agaagctgcc ggtgcagcgg ctgaagatcg accaggcctt cctgcgcgac
     3181 ctgcccgacg acgctgacgc gcgcgccatg gtcaaggcca tcatctcgct ggcgcacaat
     3241 ctcgggctga aggtggtggc cgaaggcgtg gagaccgcgg cgcagttcga gttcctgcgc
     3301 ggggccgact gcgacgaggt gcagggctgg tacctggcgc gggccatgcc cgcggcacgc
     3361 tttgcggagc tgctgcagga ggtctagcgg cgcgccggca gctcgtcgtg gtgcaggtgg
     3421 aagcggcggt tatggaacag cgacaggcag acgtcgacca ccgcggcgtc cagtgtcttg
     3481 ccgctgcagc tggcgagctc gaccagcgca ctgtccaggc cctgcgccga actgtaaggg
     3541 cggtgggtgg acatggcgac gacggtgtcg gccaccgcga tgatccgcgc ttcgagcagg
     3601 atcgcgtcgc ccttgagccc gcgcgggtaa cccgagccgt ccatgcgctc gtggtgctgg
     3661 tagacgatct cggcgatcgg ccaggggaaa tcgatgtcct tgaggatgtc gtggccggcc
     3721 agcggatgca gccgcaccat ctgcatttcc aggtcgctga ggcggccggg cttggacagg
     3781 atctccgcgg ggatcatgat ttttcccacg tcatgcaccg ccgccgccag ttcgagcccg
     3841 cgcaggcgct cgctgctcat gcccatggcc gcgccgatgg cggacgacag gtctgccgtg
     3901 cgccgctggt ggccggcggt gaacgggtcg cgctgctcca gcgtggcggc gatggcctgc
     3961 actgccgcca ccatgctgcg ctcgagccgt tgcagcgcgg actcgtgctg cgcgcgcgtg
     4021 cgcagcgcgg cgatgccata ggccagcttc tgcgcaagat cgcccagcag ggcgacttcg
     4081 tcgccggcga aggcgtcagg ctcgggcgcg tagatcatca gcgcgccata ggtttcgccg
     4141 ccgtggcaca gcggcaatgc caggctggag gcgaagccga gctgcagcgc cagcttgcgc
     4201 cacggcagca tggaaggatc gtgcgcaatg tcctcggcaa cctgcggcgt gccttcgcgc
     4261 accgcgcggc cggtaggacc acggccatgc tcgtcgtcgg cccagctgat gcggatctgg
     4321 cgcaggtaat tttccacttc gcccgaccag gcaatgggct gcaccgtgcg cccggcgtcg
     4381 tgccgcgcca tgccgaccca ggccatgcgg taaccgcccg ggtcgacgat ggccgcgcac
     4441 atgctctgca gcagcgccag ttcggtgccg gcttcgacca gcgcgctgct gcccgcgctc
     4501 agcgtgcggt aggcgcgggt gacgcgcgcc agcgccagtt ccgcacgctt gcgctcgctg
     4561 atgtcgcgca tggtgaccac gatgccgtcg acgccgcaac catggttggc atggcgcgcc
     4621 gaggattcca gcgtgatcca gtggccgagc ttgtgccggt agcggcgctg cgcgcggatc
     4681 accagcgcat gctcgtcctg caaccggctg atcgaggcga tcacctccgg gaggtcgtcg
     4741 gggtgcacca ggtcgagcag ccgcgtgccg accagttccg ccggcgcgta accaccgatg
     4801 gcctgcacgg cttggttgat gtgcaggatc acgccgcgcg catcgatcat ggcgatgatg
     4861 tcgctggcgc agccgaccat ggcccgcagc atggcctcgg tggtgcgtgg ttctgccttc
     4921 gccggatcgg tcatgacgtg cgcttcagct gccggggccg cggacttcgg ccaggacgat
     4981 tatgggtgcg cgtcgcgcat agccaagggc cgcggcgcgc ggcggcgccg gacgtcgcag
     5041 ttccgcaccg tggacggcaa ctccggcggc gccggcgcgt cgaatggaca gccactggag
     5101 aacgcgcatg ctcagaatcg ccattgtggt cggcagcacc cgcccgggcc gcaaggccga
     5161 ggccgtcgcg cagtgggtgc atggcctggc cagccggcgc aacgacgcgc aatacgaact
     5221 gctcgacatt gcgcagtacc cgctgccgct gctggacgag ccggtgccgc cgtccatgca
     5281 ccagtattcg aaggaccaca ccagggcgtg ggcggcggcg gtggcgcagt acgacggcta
     5341 cgtcttcgtc acgccggaat acaaccacgg gccgtcggcg gcgctgaaga acgcgctgga
     5401 tttcgtctac gccgagtgga acaacaaggc ggcgggattc gtcggctacg gcagcgccgg
     5461 cggcgtgcgc gcggtggagc agctgcgcca ggtgatgggc gaactgcagg tggccgacgt
     5521 gcgtgcgcag gtatcgctgt cgctgtacac cgacttcgag aacatgagcg cgttcaagcc
     5581 agcatcgatg catgaaaaga gcgtcaacac catgctcgac cagctgctgg cgtggagcgg
     5641 tgcgctgaag acactgcgcg cgcgctgaac gcgcgcagcg ccatcccgat caggccgggg
     5701 gccgaggtcc cgcggcgcgg ggcttgcggc cgccggactt gcccgtctcc agctgctcga
     5761 cctgccaggc gcagtggcgc cgctcgacgc ggcagggaac tccgccgcgg tgcgccggtc
     5821 agctttgcaa ggagcgacgg aacatgagcc acgaccgggc ccgcagaggc catagtgagc
     5881 gcgtcagcct ggcgcgccgc cacgagatcc gcgactggag ccgcgaactg gaatgcacgc
     5941 cggcggaatt gcgtgcggcg gtgagggcag tggggcgcag cgtaggcgcg gtgcgcctgt
     6001 atctggcgcg tgcgcgcgcg gcccgtgcca gccggcgcgt ggtctaccgc ggcggcgccg
     6061 cggcgcattc ctgatcaggc cgcgtaggac acgtcgagca ccaccagctc gacggctccg
     6121 gcgggcgcgt tgaaggtcac cgcgtcgccc acgcgcgcgc cgagcagcgc acgcgccagc
     6181 ggggaattcc agtggatggc gccgcgcggc ggatcggcct catcggcgcc gaccaggcgc
     6241 cagcgccggg tctgtccgcc gctggagaca tccaccgtgg cgccgaagcc aatgtcgtcg
     6301 cgcgcctgcc gcgccaccgg gacggcgctg gccacgcgcg tttccagctg ctgcacttcg
     6361 cgcgcggcga agtcgcgctc cagttcggtc atgcccagcg cggtgaggcg ccggcgcgcg
     6421 cggcccaggc gctcctgcag cttcgccatg ccgcgctcgg tgatgtagtt gggcggctcc
     6481 ggctgtggcc gcggcgtggc tgcgtcctgg gtggcgacag gggcagggct cacacagtga
     6541 tccggccagc ttgcgcttca gcggctgcgg cgcttttggc cggtggcgcg cgcacggctg
     6601 ccagcggtgg ccggcttgcg ccgcacggcg gccttgccgc ggccggcggc agatttcgcg
     6661 gcggattttt tcgcggccct ttttgctgtc ttcttcgccg gtcgctttct tcgttcctgc
     6721 tttccggcgg cgcgctgtgt cggggccttg cgcggcggcg caggcttctt cgaggcgcgt
     6781 gccgcgctgg cccgcgcgac cttcgccttg cgcttcagtt gcgcgacttc ggcgggcgcc
     6841 accggctcga tgtcggccat ctcggcctcg tccggcccgg cgccttcgcc gatggccgat
     6901 tcgtccaccg tgcgcagcgt ggagtcgaca gcatcacgca gttcgcgcgc ggccggttcg
     6961 cgcgagcggt tgtcgtcgag cagggtttcg ggagcaaggt cgtcggcggt gatgccgggg
     7021 tgcggcagct cgccgccggt ccgcccggcc agccgttcgc gctcgggcgg gaattcctcc
     7081 gccacgtcgg caggcgagcg caggtcgccc gcgcgcccgg agcgctcgtc gccggcttcg
     7141 aattccagct cgcgcggcaa ctcgtcctgc gcggagccgt ggtagcggct cgcgcgcttt
     7201 gaattaggtg ccatccctgg catgtggtac ctccctgtcg acgccagcca tccgtggcgt
     7261 tcgcgcgctc cgcgcgcggt acccgcggcc cgtccgtggc gccgcggtcc ttcctgtgca
     7321 gcactggcgc cgtcctggcg cctggccatc ctggccggga gaaatgtgcg ccgtccctgg
     7381 cgtgtcttcc tccttgtgga tgcgttcggg gttctcccaa tgcgtccccg atgacgctcc
     7441 gggggattag tcgcaacgag gttcgggaaa gttcgaaata tttttttcgg cacttttcgg
     7501 gctcaccgca cgcctgcctg gcgtgttatt aaatattata taaatattga aatagacaat
     7561 aaaaaataac accaaaacca gcaatgcggt ggaccccttg cggcctccac aggacgtttc
     7621 cgcggccgca caagttccat ccgcagcagg aaaaaccgcc gtcgcccggc agtggtcaat
     7681 aaggcatgtc cccgactgct gccgccgccg ttgccgaggt tcccgccaca tccgcggccc
     7741 tgccgctgcg cctgctcacg ctgaacctgc acaagggctt ctccacctcc ggccggcgct
     7801 tcgtgctgca tgaactgcgc gatgccatcc gcggcatttc ggcggacatc gtctgcctgc
     7861 aggaagtgct gggcgagcac cgccgccacg gcgcgcggca aggcgcacgc tggcccaagg
     7921 tgccgcagta cgaattcctg gccgactcca tctggcacca gcacgcctat ggccgcaacg
     7981 ccgtgtacga cggcggtcac cacggcaacg cggtgctgtc gaagctgccg atcgtgcgtt
     8041 tccagaacca cgacatttcc atcggcggac cggagcggcg cggcatcctg cattgcgtgc
     8101 tgcagccgca gggtcgggcg gagatccacg tgctgtgcac gcacctgggc ctgatggagc
     8161 agcaccgcca gcagcagatc gaccggctcg gcgcactgat cgacaacgcg gtgccggcgg
     8221 acgcgccgct gctgctggcc ggcgacttca acgactggcg cctgcgcggc cacgcgcggc
     8281 tgcagcgcga actgcagttg cgcgaggtgt tcgtggaggc gcagggccag tgcgcgcgca
     8341 cctttcccgc gcgctggccg ctgatgcggc tggatcgcat ctattaccgg cacgcacgcg
     8401 tgatgtgccc ttcggtgctg tcggcgcggc cgtggtcgca cctgtcggac cacgtggcgc
     8461 tgaccacgga gctcgagctg tgagcggctg gcgctggcac agcggcaacc gggtacgcct
     8521 gctggagaac ggcgaggaat attttccgcg cgtgttcgag gccatcggcc aggcgcagcg
     8581 cgaggtgatc atcgaaacct tcatcctgtt cgaggacggc gtcggccgcc ggctggagga
     8641 agcggtgatc gcggccgcgc ggcgcggtgt gcgcgtggac atcaccgtcg acggctacgg
     8701 ctcggccgac ctgtcgccgc gctttgccgc ggcgctggcc caggccggcg tacgcctgca
     8761 cgtgtttgag cccatcgtgc gcctgctcgg ctggcgcacc gggctgttcc gccggctgca
     8821 tcgcaagatc gtggtgatcg atggcgcgct ggctttcgtc ggcggcatca acttcgccca
     8881 cgaccacctg gccgaggcgg gcgaggagtc caagcaggac tacgcagtgg aagtggaagg
     8941 gccggtggtc gccgacatct accggctggt cgcgccggga cgcgcgccgc gtccgctgcc
     9001 atggtggcgg cgctggctgc tggcgcactc gcgcgcgccg atcgaggcgc cggtggtggg
     9061 cgcggtggag gcgatgctgg cgcagcgcga caaccaccat caccgcaccg acatcgagcg
     9121 cgcctaccgg ctgggcgtgc gcctggcgcg gcgcagcgtg atcatcgcca acgcttactt
     9181 ctttcccggc taccgcctgc tgcgcgacct gcgcaacgcc gcgcgccgcg gcgtgcgcgt
     9241 gcagctgatc atgcagggca agccagacat gccgtgggtg cgctggctgt cgaccagttt
     9301 ctacggttac ctcgcgcgcg gcggcgtgga gatctacgag tattgccagc ggccgatcca
     9361 tgccaaggtg gcggtgatcg acgacgaatg ggccacggtg ggatcaagca atctcgatcc
     9421 gctgtcgctg tcgctcaacc tcgaggccaa cctggtgctg cgcaaccgcg agttcagcct
     9481 gcagctgcgc cagcggctcg agcggctgat gagcgaatcg tgcacgcggc tcgaacaggc
     9541 gcagttccgc cggcgcggcc cgttctggca gctggtcacg gtgctggcgt tccatgccgc
     9601 gcgccgcttc cccgcggtgg cgggctggct gccggcgcac cgaccggtgc gcaccatgct
     9661 caccggcgaa ggcctggccg tgccaacgca gcgcaaggcc gcgtgaactt tgccgcgggc
     9721 cttcttccta attcgtgcac acgcaaacag gaggaaaaca tgaaacgcat gcaatcgggc
     9781 cccgtcgccc tctgcctcgt gctggccgtg gccggcgcct acgccggcag cagcacggac
     9841 ccgaccaagg cgtccggccc caccaccgtg aaggaagacg atcttgaatt catgaccaag
     9901 gccagccagg ccggcatggc cgaggtgcag gaggcgcagg tggccagcac caaggcgatg
     9961 gacgcgggcg tgcgcagctt tgccca
//
LOCUS       DASZZC010000057         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_516668,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000057 DASZZC010000000
VERSION     DASZZC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516668"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /locus_tag="VHE37_01440"
     CDS             complement(<1..2019)
                     /locus_tag="VHE37_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HVT34219.1"
                     /translation="MQMESAASSAAEARRPLGTELPVPAAELAATAPGGIKVIRRNGK
                     VTSFDGSKIQIALTKAFLAVEGGSAAESSAVRQKVEALTAQVVAGLTRHITGGGTVHI
                     EDIQDQVELALMRAGEHKVARDYVIYREQRAQKRAAEAAAKPAAAKAATRLTVKLDDG
                     NMVPLNEERMQRVVREACANTSGVDGDEIIAAALRDLYDGIPEAEVAQAVTLAARARI
                     EKEPNYTYVSARLLLDALRHEALKFLNMAETRPTFEEMKKIYPDYFREYIHRAVKLEL
                     LDSKLALYDLDKLGKALLAERDAKFDYLGLQTLYDRYFLHSNKTRFELPQAFFMRVAM
                     GLAMNEIDRETRAIEFYTLLSSFDFMSSTPTLFNSGTLRPQLSSCYLTTVPDDLHGIF
                     GAIHDNAMLSKFAGGLGNDWTPVRGIGGYIKGTNGKSNGVVPFLKVANDTCVAVNQGG
                     KRKGALCAYLETWHRDIEEFLELRKNTGDDRRRTHDMNTANWVPDLFMRRVMEDAQWT
                     LFSPDDTPDLHDLTGAAFEKRYTEYEKLADEGKIKNIKRLPATQLWRKMLSMLFETGH
                     PWVTFKDPCNLRSPQQHVGVVHSSNLCTEITLNTKAGQEIAVCNLGSVNMPAHVVDGK
                     LDLGKLEKTITTAMRMLDNVIEYNYYSVATARNSNLRHRPVGLGVMGFQ"
     gene            2470..>3774
                     /locus_tag="VHE37_01445"
     CDS             2470..>3774
                     /locus_tag="VHE37_01445"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HVT34220.1"
                     /translation="MKLLPVLLAGGSGTRLWPLSREQYPKQFLKLVSERSLLQETGLR
                     AQALPHALPVLAICSDAHRFLVAEQLLEAGCQGAHVMLEPSGRNTAPAAAAAAHYAVE
                     KHGPDTLVFLMAADHVIPDQAAFMASVKTAAAAAQSGAIVTFGIQPTAPETGFGYIKV
                     GAPMGNGAHKIAAFVEKPPLDQARAFIAEGGYYWNGGMFMFRAQSFLEELNRLEPEMH
                     AAAADAVTRSKRDLDFVRLDPDSFKRCRNESIDYAVMEKTRHAALVPLDAGWNDVGSW
                     SFLDALPKDEQGNVVRGDVLMEESGGNLVHAESRLVALAGVQDHIVVETQDAVLVTTR
                     ARAQDVKKIVASLKASKRVEANDHPRVFRPWGSYETIALAEGFQVKRIIVKPGQKLSL
                     QMHHHRAEHWIVVRGTARVTCEDKEFVLHEDQSTYIPLGHKHR"
BASE COUNT          629 a         1178 c         1243 g          724 t
ORIGIN      
        1 ctggaagccc atcacgccga gtccgaccgg gcggtggcgc aggttggagt tgcgcgccgt
       61 ggccaccgag taatagttgt actcgatgac gttgtcgagc atgcgcatcg ccgtggtgat
      121 ggtcttctcg agcttgccca ggtcgagctt gccgtccacc acgtgcgccg gcatgttgac
      181 cgagcccagg ttgcacacgg cgatttcctg gccggccttg gtgttcagcg tgatctcggt
      241 gcacaggttg gagctgtgca cgacgccgac gtgctgctgc ggcgagcgca ggttgcaggg
      301 gtccttgaag gtgacccacg ggtggccggt ttcgaacagc atgctcagca tcttgcgcca
      361 cagctgggta gccggcaggc gcttgatgtt cttgatcttg ccctcgtcgg ccagcttctc
      421 gtactcggtg tagcgcttct cgaacgcggc gccggtgagg tcgtgcaggt ccggcgtgtc
      481 gtcgggcgag aacagcgtcc actgcgcgtc ttccatcacg cggcgcatga acaggtcggg
      541 cacccagttg gcggtgttca tgtcgtgcgt gcggcggcgg tcgtcgccgg tgttcttgcg
      601 cagttcgagg aattcctcga tgtcgcgatg ccaggtctcg aggtacgcgc acagcgcgcc
      661 cttgcgcttg ccgccctggt tgacggcgac gcaggtgtcg ttggccactt tcaggaacgg
      721 cacgacgccg ttcgatttgc cattggtgcc cttgatgtac ccgccgatac cacgcaccgg
      781 ggtccagtca ttgcccaggc cgccggcgaa tttgctcagc atggcgttgt cgtggatcgc
      841 gccgaagatg ccgtgcaggt catccggcac cgtcgtgaga tagcagctcg acagctgcgg
      901 gcgcagcgtg ccgctgttga acagcgtagg cgtgctgctc atgaaatcga acgacgacaa
      961 cagcgtgtag aactcgatgg cacgcgtctc gcggtcgatc tcgttcatcg ccaggcccat
     1021 ggccacgcgc atgaagaagg cctgcggcag ctcaaagcgc gttttgttgg agtgcaggaa
     1081 gtagcggtcg tacagcgtct gcaggcccag gtagtcgaac ttggcgtcgc gctcggccag
     1141 cagcgccttg cccagcttgt ccaggtcata cagcgccagc ttggagtcga gcagttcgag
     1201 cttgaccgcg cggtggatgt attcgcggaa gtaatccggg tagatcttct tcatttcctc
     1261 gaaggtcggc cgcgtctcag ccatgttgag gaacttgagc gcctcgtggc gcagcgcgtc
     1321 gagcagcagg cgcgccgaca cgtaggtgta gttgggttcc ttctcgatgc gcgcgcgggc
     1381 ggccagcgtc accgcctgcg ccacctcggc ctcggggatg ccgtcgtaca ggtcgcgcag
     1441 cgcggcggcg atgatttcgt cgccgtcgac gccgctggtg ttggcgcagg cttcgcgcac
     1501 cacgcgctgc atgcgttcct cgttgagcgg gaccatgttg ccgtcgtcga gcttcaccgt
     1561 caggcgcgta gcggccttgg cggccgcggg cttggccgcc gcttcggcgg cgcgcttctg
     1621 tgcgcgctgc tcgcggtaga tgacgtagtc gcgcgcgacc ttgtgctcgc cggcgcgcat
     1681 cagcgcgagt tcgacctggt cctggatgtc ttcgatatgc acggtgccgc cgccggtgat
     1741 gtggcgggtg agcccggcca ccacctgcgc ggtcagcgcc tcgaccttct ggcgcacggc
     1801 ggaactttcc gccgcgctgc caccttccac ggcgagaaac gccttggtca gcgcgatctg
     1861 gattttcgat ccatcgaagc tggtgacctt gccgttgcgg cggatcacct tgatgccgcc
     1921 cggcgcggtg gccgcgagtt ccgcggcggg caccggcaac tcggtaccca ggggacggcg
     1981 tgcttcagcg gcagacgatg cagcagattc catttgcatg atgggattcc cctctcttta
     2041 ttcggcgtaa aacgagttcc tgtggcagcg ctcctgcttt cgcgcgggga gtctttgcca
     2101 gtctggcaac aatatattgt ggttcggccg cgcgtcaatc ccaacaattt gtgtccaaga
     2161 cggtgtgcag cgcggggaaa aactgtgcat gacccgtgga taagccgtca ctccccaggc
     2221 aagttcctga tgcgcaaagc agcctttcac gagccgcgga aatgttgacc gcatcggcgt
     2281 ggatcatgtt atttattgat gtattatttt atttatatat tatatttact aacatgaaga
     2341 cggcacttgc ggtggagcaa tgccaactgc atctcgccgc ataaaacaat cctccaatcc
     2401 ggccatcgaa acacccggcg cggtcaccgt aaaatgcggg ccgattttcc acatgcggac
     2461 ttccctttca tgaaattgct tcccgtgctc ctcgccggcg gctccggcac tcgtttgtgg
     2521 ccgctgtcgc gcgagcaata tcccaagcag ttcctcaagc tggtgagcga gcgcagcctg
     2581 ctgcaggaaa ccggcctgcg cgcgcaggcg ctgccgcatg cactgccggt gctggccatc
     2641 tgcagcgacg cccaccgctt cctggtggcc gagcagctgc ttgaagcggg ctgccagggc
     2701 gcacacgtga tgctggagcc gagcggccgc aacactgcgc cggccgcggc ggccgcggcg
     2761 cattacgcgg tggagaagca cggccccgac acgctggtgt tcctgatggc ggccgaccac
     2821 gtcatccccg accaggccgc gttcatggcg tcggtgaaga ccgctgccgc cgcggcgcag
     2881 agcggcgcca tcgtcacctt cggcatccag cccaccgcgc cggaaaccgg cttcggctac
     2941 atcaaggtgg gtgcgccgat gggcaatggc gcgcacaaga tcgcggcgtt tgtggaaaag
     3001 ccgccgctgg accaggccag ggccttcatt gccgagggcg gctattactg gaacggcggc
     3061 atgttcatgt tccgcgcaca gtcgttcctg gaagaattga accggctgga gccggagatg
     3121 cacgccgccg cggccgacgc ggtgaccaga tccaagcgcg atctcgactt cgtgcggctc
     3181 gacccggaca gcttcaagcg ctgccgcaac gaatcgatcg actacgcggt gatggaaaaa
     3241 accaggcatg cggcgctggt gccgctcgat gccggctgga acgacgtggg ttcgtggagc
     3301 ttccttgacg cgctgcctaa ggacgaacaa ggcaacgtgg tgcgcggcga cgtgctgatg
     3361 gaagaatccg gcggcaacct ggtgcatgcg gaatcgcgcc tggtggcgct ggccggcgtg
     3421 caggatcaca tcgtggtgga gacgcaggac gcggtgctgg tcaccacccg cgcgcgcgcg
     3481 caggacgtga agaaaatcgt cgcctcgctc aaggcgagca aacgcgtgga ggccaacgac
     3541 catccgcgcg tattccgccc gtggggctcc tatgaaacca tcgcgctggc cgagggcttc
     3601 caggtcaaac gcatcatcgt caagccgggt cagaagctct cactgcagat gcatcaccac
     3661 cgcgccgagc actggatcgt ggtgcgcggc accgcccgcg tgacctgcga ggacaaggag
     3721 ttcgtgctgc acgaggacca gtccacgtat atcccgctgg gccacaagca ccgg
//
LOCUS       DASZZC010000058        12530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1226010,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000058 DASZZC010000000
VERSION     DASZZC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226010"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VHE37_01450"
     CDS             <1..654
                     /locus_tag="VHE37_01450"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HVT34221.1"
                     /translation="KLDVPAYPGDLLYMGVTVQTQAGNGLKDLPVQISSRKGNPYVLM
                     AERTDQDGFLEFHLVANQVGEDVVTVTAAGVQRDFLVSVTPPPRSQWLGGLDLKGVTS
                     WDLLMSANVTVTRDRVSAIYPPLLQALKGKTVRLVGFMLPLAVNEKQDHFLLSANPPA
                     CFFHPPGGPSSAIEVFADRPVEMDDEAMVVEGRFELIKNSADGVLYRLRDAHRISKS"
     gene            688..1011
                     /locus_tag="VHE37_01455"
     CDS             688..1011
                     /locus_tag="VHE37_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34222.1"
                     /translation="MKLTIRVTTFAVLLAAAIPAASWARGNVDDVPGVEDGPALSASA
                     ARSMDLGFTELHTDRGSIADGGAMSTSYRTPDGKLVELQMDPISGEITQSDKDAAVQR
                     RQPAR"
     gene            1068..3101
                     /locus_tag="VHE37_01460"
     CDS             1068..3101
                     /locus_tag="VHE37_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT34223.1"
                     /translation="MLPRFRAARRYELPLLGGAVVLIIAALATPAAARMVEAALWLAA
                     AGFLSACMMAFARARLAEGESVERKASKVGSKPPSAPKPQAIRDWPAHLQHLVLRGIA
                     DWIPDVLSLSEWPQSLVALGSGSLAVHFIVQWWRLAQHAGDPAALRLPGVILVLSAFP
                     LLVLQRMHAGLSADLLPEAPQLERLLRLPLAACLGQGGVMFAIAGGFGWAIQVERIIA
                     AVVLAAAVEMLLRGAVTVFVPWPPIERRLAIADSTVAGLLLRLRPPTLQSLNTTVRQR
                     FGINLSRSWALSFVQKAALPLVAGIGLVTWLFTGVTALGINQRGVYERLGEPAEVLGP
                     GVHLHLPWPLGILRTVEIGVVHQLPIEFVLADQNGQVPAQAEEEEQRVAAEADPPASA
                     DRLWDDAHPYEGSYLIASEDNARQSFQLVDVDMAVLYQIGLSDEAARNALYRVTNVED
                     VIQALSGQILVRYLAQHQLLELLGESRETLSADFRTRLQQELDLFHSGVDVLAISIEA
                     IHPPPGAASAYHDVQAAEIRAGTHVAGSLGNAARTQQGSQTLATRARNDAKAAAVERV
                     DQARITSTLFGGDLQSYVKDGDAFLLERWLDNLAAGLAKTKVVIIDHRLKDSEVPTVD
                     LRTPLADQRNPSLDATADAQSSSPPSQSLEPPAGNPAMQQQLNPDYEGVKGDE"
     gene            3098..4126
                     /locus_tag="VHE37_01465"
     CDS             3098..4126
                     /locus_tag="VHE37_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HVT34224.1"
                     /translation="MNDQDSARPADPQPKVAVVMVKAWLARYGRYIAAGFIVLAAFLA
                     ACVFLIVPGEAIVVTRMGDPVRVITQPGLAWKIPAPVENTIAVDLRLNTTSSGLQDVG
                     TRDGLRILVQAYVAWQVPDDPAHIRQFLRAVQNQPDVAAEQLRSFIGSMLEITVSSFD
                     LASLINTDPAKIQLSQLETRLRERIDQEALKVYGVTVRQVGIERLTLPAETLNATVSR
                     MRAERATVAAQREADGQRRAEEILSNADRDSRVVVAQAKADAADVEAKARVEAADIYG
                     KAYAGNRELYTLLRSLDTLDAVLGDNTRLILRTDAAPFRVLVDGPGRSAGTVPSPKPS
                     RAARSPSP"
     gene            4123..5142
                     /locus_tag="VHE37_01470"
     CDS             4123..5142
                     /locus_tag="VHE37_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT34225.1"
                     /translation="MTRAGDSRLEPDSAWAQSLALSFRFLFLVVYLLAFLWAVSNVRI
                     VPTDRRAVVLRFGSIAREQGPGLLLAFPEPIERIIVLPSSDRQNQYIVRTLANSPNML
                     SGDPRWNTGMFLSGDMSVVHLKATLFYRITDATAYVLSVKHVEPALERLFLASAISVC
                     GARDLDTILVARPELETAAGSSRAGRERLRTDLMDEINRRLSDLAQQGASLGITVGRV
                     DLLPEIPGEAEQAFNSVLVAIQSAETRVAEARTRAEQTMQHANQEKDRIVADAQARAA
                     ERVTTAQTRTAAIAALARGNPGLQGQALIDQIYADRIGPLLAKAAQVQTISGGAHVIL
                     PGALP"
     gene            5139..7049
                     /locus_tag="VHE37_01475"
     CDS             5139..7049
                     /locus_tag="VHE37_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HVT34226.1"
                     /translation="MTSLAAPEVATGDTHLLSAAEQWSLGVRLTLSLAAAGCLVVASL
                     IGLMRPGQQDVAELAAGLGAALVAVPALAAAWHSLRRPSLHGITDQLIALALLAAWAA
                     GDLFTAALLPLVMMLGHILEERSLLGSQEAIRALSRLTETAARRLSPSGLVEEVAARA
                     LRVDDRIELRAGDRVPADGIVETGQSSVDTASITGESVPEEVVPGSEIYNGSINLDGH
                     LIVRITRVGAETTLGRVVALLRDAEQAKPPVTRLLERYSQRYLILVLLLAAGTWFATG
                     KTAVVLAVLVASCPCALVLAAPATSIAALSVASRHGILVKGAAFLETLASVDAVAFDK
                     TGTMTIGQLKLAGVETEPGIRKTELIALAANLGTASSHPVSRALAAGLADGERLNLED
                     IQETRGLGVTARLGGERVAMGRAALFESLQIAASAPPTHDGPVAGVARGKRFLGWVLL
                     ADEPRPEAATAVDDLRALGLDRQLLITGDRWPAARRVAEAIAVTDVQAELLPAQKMEC
                     VLQLSSQGYRPLVVGDGINDALALKAGAVGVAMGGHGTDVALASADLVLMTNDLRRLG
                     TCIRLSRRCRRTIYINIGLGLIWTMVMLFLAVTGVLGVHGALIAALAQNFSSVLVMIN
                     AGRLLKFQELLS"
     gene            complement(7449..8237)
                     /locus_tag="VHE37_01480"
     CDS             complement(7449..8237)
                     /locus_tag="VHE37_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34227.1"
                     /translation="MPIADVNGQKIHFEDTGGSGPVVIFSHGLLMDREMFAPQVAALR
                     GRYRVITWDERGHGLTGDAREAFSYYDSANDLAALLTHLGIDRAVLAGMSQGGYLSLR
                     AALTHPGLASGLVLIDTQARPEDPATLPVYQQMIATWAAQGLSDEMAATIEHIILGAG
                     YADAPKWKAKWRKNSPANLQQIFSTLATRDDISGKIAAIKVPALVIHGDADAAIPLAH
                     AQEMARALPRSQLHVVAGAGHAANLTHAAQVNPAIEAFLSEHAG"
     gene            complement(8262..9317)
                     /locus_tag="VHE37_01485"
     CDS             complement(8262..9317)
                     /locus_tag="VHE37_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT34228.1"
                     /translation="MDGPEESDASPEPLAYPWAQAPVPGHAVEVADGVLWLRMPLPFA
                     LDHINVWALRDGDRWTVVDTGMQGAQTLAAWESAMAGPLQGLPVTRVISTHMHPDHVG
                     MAGWFTRRFGCRLWMTRLEYLTCRVLVADTGREAPADAVRFYAAAGWSERALEHYRTR
                     FGGFGKGVYTLPDSYRRVVDGDVISIGGRDWRAVIGRGHSPEHLCLHCPELRVLISGD
                     QVLPRISSNVSVFPTEPDGDPLAEWLDSLALVARMVPDDVLVLPAHNLPFRGLHRRLA
                     HLQRGHERGLARVREALAEPRRVVDLFSALYKRPIGDELLGLATGEALAHLNYLLRRG
                     QALRRTDEHGIHWYSRL"
     gene            complement(9346..10110)
                     /locus_tag="VHE37_01490"
     CDS             complement(9346..10110)
                     /locus_tag="VHE37_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT34229.1"
                     /translation="MATKLFDLTGRVALVTGASRGIGEATARLLAEQGAHVVVSSRKQ
                     EDLDKVAASIIATGGKATAIAAHQGDSAALKHLLGEIVGKLGRLDILVNNAATNPYMG
                     HIADTDMGMVDKTLQVNIKGYFELSALAAKQMKNNGGGAIVNIASINGIKPGPLQGIY
                     SITKAAVINMTLAFAKECAPQKIRVNAVLPGLTDTKFASALTKNEAMLKHFLPFIPLG
                     RVAQPAEIAPGILYLVSDAASYVTGTTLTIDGGFLA"
     gene            complement(10194..11264)
                     /locus_tag="VHE37_01495"
     CDS             complement(10194..11264)
                     /locus_tag="VHE37_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HVT34230.1"
                     /translation="MTDTTLDQAGAVRDENNFDPALILPFLQQHIPGLRGAATLQQFQ
                     GGASNVTYLLRVGEREMILRRPPAGTKAKSAHDMGREFRVMQRLSPHFPCPRPLAYCE
                     DDSLIGSTFYVMEKITGVILRRDPPKGISYTPEAARQLCLNLLDTQIRLHTLDYDQLG
                     LSDMGKPTGYVERQVNGWNERFGKAWTDDVPRCENIMAWLAAKKPADSPRPGIIHNDY
                     RFDNVVLSPSDHQSIIGVLDWEMATLGDPLMDLGSSLAYWVQNSDSKQMQALRMQPST
                     LPGMLTREEIVRYYGEKTGREISNTDFYYVFGLFRLAVIAQQIYYRWKMGQTKNPRFQ
                     TFGMFVTVLSQVAGNVIDKSKL"
     gene            complement(11272..11970)
                     /locus_tag="VHE37_01500"
     CDS             complement(11272..11970)
                     /locus_tag="VHE37_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVT34231.1"
                     /translation="MTTLVLVRHGQASFGAASYDALSENGRRQSFVLGEHWKRTGFDM
                     DGLYCGDMVRQKTTGLHALEAMQRSSAVTTHSAFNEYDFDGILRGYLPVVAREEPAIS
                     IERKELYANPRAFQLVFEKAIALWLSGREPDGGIALEGWSRFRARVLAGLREVAAPGR
                     KQVVVFTSGGVIAVALQQALGLTDEMTFRMNWRIYNASVHTFRVGRSGLSLTGFNNIT
                     PLELAGDAALLTFR"
     gene            complement(12083..>12530)
                     /locus_tag="VHE37_01505"
     CDS             complement(12083..>12530)
                     /locus_tag="VHE37_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34232.1"
                     /translation="LDMSFASREEYLAYWRKHPALASDWSDDLVQYFDHDLVGTAPAL
                     RSSTSKDAVIGDTRSMMGDDLIARSLLQLKMPVRLLRAPRGLQDGKPLYSEALVRRWA
                     ARIANFSSTTIADVNHYTIIMSARGADAVTRELRALAEPAAVADAA"
BASE COUNT         2061 a         4044 c         4194 g         2231 t
ORIGIN      
        1 aagctcgacg taccagccta tccaggcgac ctgctttaca tgggggtgac cgtgcagacc
       61 caggccggaa atggtctcaa ggatctgccg gttcaaatca gctcccgcaa gggcaacccc
      121 tacgtgctga tggccgagcg cacggatcag gatggcttcc tggaatttca tctggttgcg
      181 aatcaagtcg gcgaagacgt ggtcaccgtt actgcagccg gcgtccagcg cgacttcctg
      241 gtcagcgtca cgccgccgcc gcgcagccaa tggctcggcg gcctcgattt gaaaggcgta
      301 acctcatggg acctgctgat gagcgccaac gtaaccgtca cccgtgatcg cgtttcggca
      361 atctacccgc cgctgttgca agcgctcaag ggcaagaccg tgcgcctggt tggattcatg
      421 ctgccgctcg ctgtcaatga aaaacaggat catttcctgc tgtcggccaa tccgcccgcc
      481 tgcttcttcc atcccccggg aggcccgtca tcggcaatcg aagtattcgc tgacaggccg
      541 gtggagatgg acgacgaggc aatggtcgtc gaaggcagat ttgaactgat caagaacagc
      601 gccgatggcg tcctctacag gctacgtgat gcacaccgca tatccaagag ttgatgcggt
      661 tcgattcaac ccacaggaga agtgacaatg aagctgacta tccgcgtgac gactttcgca
      721 gtgctgctgg ccgccgcaat tcccgcggcc agttgggcac gaggcaacgt cgatgatgtg
      781 cctggcgtcg aagacggccc tgcgctgagc gcaagtgcgg cgcgcagcat ggaccttgga
      841 ttcaccgagt tgcacaccga caggggcagc atcgccgatg gcggcgccat gtcgacttcc
      901 tatcggaccc ctgatggcaa gctcgtcgag ctgcagatgg acccgatcag tggcgagatc
      961 acgcaatccg acaaggatgc ggccgtccag cgtcgtcagc ccgccaggta gcatgacgct
     1021 ggtgcgcgag agtgactagg acgactccgg aacccagcgc cggaggcgtg ctgccgaggt
     1081 ttcgtgcggc gcggcgttat gaactgcctc tccttggcgg cgcggtcgtt ctgatcatcg
     1141 cagcgctggc cacgcccgct gcggccagaa tggtggaagc tgcgctgtgg ctcgccgcag
     1201 cgggcttcct ctcggcgtgc atgatggcgt tcgcacgggc gcgacttgcc gagggcgaga
     1261 gcgttgagcg caaagcaagc aaggtgggtt cgaagccgcc gagcgcacca aagccgcagg
     1321 cgattcgaga ttggcccgct cacctgcaac atctcgtttt gcgcggcatt gccgactgga
     1381 ttcccgatgt tctgtcgttg agcgaatggc cgcagtcgct ggtagccctc ggatcgggtt
     1441 cccttgctgt gcatttcatc gtgcaatggt ggcggctggc gcaacacgct ggcgatcctg
     1501 ctgcgttgcg attgcccggc gtcatcctcg tgctgtctgc gttccccctg ctggtgttgc
     1561 agaggatgca tgcaggcctg tccgcggacc tccttccaga ggcgccccaa ctcgagcgcc
     1621 tgctgcgcct gccgctggca gcctgccttg gccaaggcgg cgtcatgttt gccatcgccg
     1681 gcggattcgg ctgggccata caggtcgagc ggatcatcgc cgcagtcgtg ctggccgccg
     1741 cggtggaaat gctcctgcgc ggtgcagtca ccgtattcgt gccgtggccg ccgatcgagc
     1801 gcaggctggc gatcgcggac agcaccgttg cgggtctgct gctgcggctc aggccgccta
     1861 cgctccaatc gctcaacacc acggtacgtc agcggtttgg catcaatctg tcgcgcagct
     1921 gggcgctgtc ctttgtacaa aaggcggctt tgccgctggt ggcaggcatt ggcctggtta
     1981 cctggctgtt caccggcgtg acggcactgg gcatcaatca gcgcggtgtg tatgagcgcc
     2041 tgggcgagcc cgccgaggtt ctcggccccg gcgtgcatct gcacctgccg tggccgctcg
     2101 gcattctgcg caccgtcgag atcggcgtgg ttcaccagct gcccatagaa ttcgtactgg
     2161 ccgatcaaaa cggccaggtc cccgcacaag cggaggaaga ggaacaacgg gttgcggcgg
     2221 aggcagatcc gcccgcaagc gcggaccgtc tgtgggatga cgctcacccg tacgagggaa
     2281 gctacctcat cgccagcgag gacaacgccc ggcagagctt ccagctggtg gacgtggaca
     2341 tggccgtgct ttaccagatc gggctatcgg acgaagcggc ccgcaatgcg ctgtatcgcg
     2401 tgacgaacgt ggaggacgtc atccaggcgc tttccggcca gattcttgtc cgctacctcg
     2461 cgcagcatca actgctggaa ctgctgggcg agagccgcga aacgctgtcg gcggacttcc
     2521 ggacccgcct gcaacaggaa ctggacctgt tccattccgg tgtcgatgtg ctcgccatct
     2581 cgatcgaagc cattcatccg ccgcccggcg cggcgagcgc ctaccatgac gtgcaggcgg
     2641 cggagatccg tgccggcacc cacgtcgccg ggtcacttgg caatgccgca cgcacgcagc
     2701 agggttccca aacactggca acgcgcgcgc gcaatgatgc gaaagccgcg gcggtcgaga
     2761 gggttgacca agctcggatc accagcacgc tgtttggcgg cgacctccaa agctacgtaa
     2821 aggacggtga cgcattcctg ctggaacgct ggttagacaa cctggccgcg gggctggcga
     2881 aaaccaaggt cgtcatcatc gaccatcggc tgaaggacag cgaagtcccg accgtggatc
     2941 tgcgcacacc gctggcggat cagcgcaacc cgtcgctgga tgcaactgcc gatgcgcagt
     3001 cgtcgagccc gcccagccaa tctctcgaac ctcccgccgg aaatccggcg atgcaacaac
     3061 aattgaatcc cgactacgag ggagtgaagg gtgatgaatg aacgatcaag actccgcccg
     3121 gcccgctgac ccgcagccca aggtcgcggt cgtgatggtc aaagcctggc ttgcccgcta
     3181 cggccgctac atcgcggcgg gtttcatcgt gctggcggcc ttccttgctg catgcgtgtt
     3241 cctgatagtg ccgggcgagg cgatcgtcgt gacgcgtatg ggcgatccgg tccgcgtcat
     3301 tacgcagccc gggctggcct ggaagattcc ggcgccggtc gagaacacca tcgccgtgga
     3361 cctgcggctg aacacgacct ccagcggatt gcaggacgtc ggtacccggg atggcttgcg
     3421 catcctggta caggcttatg tcgcatggca ggtgccggat gaccctgcgc atatccggca
     3481 gttcctgcgc gcggtccaga accagcccga cgtggccgcc gaacagctgc gcagcttcat
     3541 cggttcgatg cttgagatca cggtgagcag ctttgacctg gcatcgttga tcaacaccga
     3601 tcccgcgaag atccagctgt cacaacttga gacccggctg cgcgaacgca tcgaccagga
     3661 agcattgaag gtatatggag tgacggtgcg ccaggtcggg atcgagcgct tgacgctccc
     3721 ggccgaaacc ctgaatgcca ccgtgtccag gatgcgcgcc gaacgcgcca cggtcgccgc
     3781 gcagcgcgaa gccgatgggc agcggcgcgc ggaggagatt ctctccaacg ccgatcgcga
     3841 ttcccgcgtg gtggtggccc aggccaaggc cgatgcggcc gatgtggaag ccaaggcgcg
     3901 ggtcgaggca gcggatatct acggcaaggc ctatgccggg aatcgcgagc tctatacctt
     3961 gctgcgctcg ctcgacacgc tcgatgcggt gctcggcgac aacacgcggc tgatcctgcg
     4021 caccgatgcg gccccgttcc gggtgctggt cgatggcccc ggcaggagcg caggcactgt
     4081 gccgtcgccg aagccgtcac gcgccgccag gagcccgtcg ccgtgacgcg ggcgggcgac
     4141 agtcgcctcg aacctgatag cgcctgggca caatccctgg cgctgtcctt ccgcttcctg
     4201 ttcctcgttg tctacctgct ggcattcctg tgggcggtat cgaatgtccg tatcgttccg
     4261 accgatcgtc gcgcggtagt gctgcgcttt ggcagcattg cccgcgaaca gggacccgga
     4321 ttgctgctcg cctttcctga gccgattgaa aggatcatcg tgctgccgtc ttccgatcgc
     4381 cagaatcagt acatcgtccg caccttggcc aactcgccga atatgctcag cggcgatccg
     4441 cgctggaata cgggcatgtt cctgtcgggg gacatgagcg tggttcacct gaaggcgacg
     4501 ctgttttacc ggatcacgga tgccaccgcc tacgtgttgt cggtcaaaca cgttgaaccg
     4561 gcgctcgagc ggctgttcct ggccagtgcg atttcggtct gcggcgcccg tgacctcgat
     4621 accatcctgg tggcgcgtcc ggaattggaa acggctgccg gttcgtcgcg tgccggtcgc
     4681 gagcggctgc gcaccgacct gatggatgaa atcaatcggc gcctgtctga tcttgcgcag
     4741 cagggagcga gcctgggcat caccgttggc cgcgtcgacc tgctgcccga gatcccggga
     4801 gaagccgagc aggcattcaa ttcggtgctg gtggcgatcc agtcggcgga aacccgggtc
     4861 gccgaggcac gcacgcgggc cgagcagacc atgcagcacg cgaaccagga aaaggaccgc
     4921 atcgtcgccg acgcccaggc gcgcgcagcg gagcgggtga cgactgctca aacccgcacc
     4981 gccgccattg ccgcgctcgc tcgtggaaac cccgggctcc aggggcaggc gctcatcgat
     5041 caaatctatg ccgatcgcat cggcccgttg ctagccaagg cggcgcaggt gcaaaccatt
     5101 agcggcggcg cccacgtcat tctgccggga gcattgccgt gaccagcctt gctgctcccg
     5161 aggtcgccac aggcgacacg cacttgctca gcgcggccga acaatggtcg cttggcgtgc
     5221 gattgacgct ctcgctggcg gccgcgggct gcctggtggt cgcatcgctc atcggcctga
     5281 tgcggcccgg gcagcaggat gtcgccgagc tcgcggccgg gctgggtgcg gcgctggtcg
     5341 cggtcccggc gctcgcggcc gcctggcaca gcctgcgccg cccgagcttg cacggcatca
     5401 ccgatcagtt gatcgcgctg gccctgctcg ccgcctgggc agccggcgat cttttcacgg
     5461 ccgccctgct gccgttggtg atgatgctgg ggcatatcct tgaagagcgc tcgctgctgg
     5521 gctcgcagga agcgatccgg gcactcagcc ggcttacgga gacggcggcg cgccggctat
     5581 cacccagcgg cctggtcgag gaggttgcgg cccgggcctt gcgcgtcgat gatcgaatcg
     5641 agttgcgcgc cggcgaccgg gttcctgccg acggcatcgt cgagaccggc cagtccagcg
     5701 tcgatactgc atcgattacg ggagaatcag tgccggagga ggttgttccc ggatcggaga
     5761 tttacaacgg atccatcaac ctcgatgggc atctcatcgt tcgcatcacg cgggtcggcg
     5821 ccgagaccac gctggggcga gtggtcgctc tgctgcgcga tgccgagcaa gccaagccgc
     5881 cagtgacgcg gctgcttgaa cgctattcgc agcgctacct gatcctggtg ctgttgctgg
     5941 cagcgggcac atggttcgcc accggcaaaa ccgccgtcgt gctcgccgtc ctggttgctt
     6001 cgtgcccgtg cgcgctggtg ctcgcggcgc ccgcgacttc cattgcagcg ctgtcggtcg
     6061 ccagccggca cggcatcctg gtcaagggcg cagcgtttct cgaaaccctg gcgtcggtgg
     6121 acgccgtggc attcgacaag accggcacca tgaccatcgg ccaattgaag ctggccggcg
     6181 tggagacgga gcctggcatc aggaaaacgg aattgatcgc acttgccgcc aatctcggga
     6241 cggccagcag ccatccggtc agccgggcac ttgcggcggg cctcgccgat ggcgaacggc
     6301 tgaatctgga agacatccag gagacgcgcg ggctgggcgt cactgcgcgc ctgggcggag
     6361 aacgggtcgc catgggacgc gccgccttgt tcgagtcact gcagatcgca gcatcggcgc
     6421 cgcctacgca tgacggcccc gtcgccggcg tggctcgcgg caagagattc ctcggttggg
     6481 ttctacttgc ggacgaaccg cggcctgagg ccgcgaccgc ggtagacgat ctgcgcgcgc
     6541 tgggcttgga ccgccagctt ctcatcacgg gcgaccgttg gcccgcggca cgccgggtcg
     6601 ccgaagctat cgcggttacg gacgtccagg ccgaattgct gccggcgcag aagatggagt
     6661 gcgtgctcca gctctcctcg cagggctatc ggccgctggt cgtcggcgac ggcatcaacg
     6721 atgcgctcgc gctcaaggcg ggcgcggtcg gcgtcgccat gggcggacat ggcaccgatg
     6781 tggccctggc ttcggctgat ctggtgctga tgaccaacga tttgcggcgc ctgggcacct
     6841 gcatccgcct tagccgtcgc tgccgccgca ccatctacat caacatcggc ctcgggctga
     6901 tctggaccat ggtcatgctt tttctggcag taaccggtgt tctcggtgtg cacggcgcgc
     6961 tgattgctgc cctggcgcag aacttcagca gcgttctggt gatgatcaac gcaggtcgac
     7021 tgctgaagtt ccaggagttg ttgtcgtaag gcctgtcgcc ggttacatcg cggctcggtc
     7081 gcggtgtaag ggtgtttggg ccgcggctca gcttcagtaa ccaaagagct tcagccgctc
     7141 acgcatcttg gcggtcccga tctcgcgttg tcgctgaatc tcctcttcat gctccgaacc
     7201 cacgagccat gacgagagat cgcagggctt ggggagattc acgccaatca cctgatcgac
     7261 agccgggcag ccgaggcggg cggcgctgga gcgtacgctg gcgttcctgc acgaacagtt
     7321 gaaatgagcg cagcgggtgc gctttttggc cgaccgcact gcgtatggga ctgttataaa
     7381 atatcttata tttgttaaaa cagtcaatat ttaataacag gcaaaacggc catgcggtga
     7441 gagatttttc agcccgcgtg ctcgctcagg aatgcctcga tggccggatt cacctgtgcc
     7501 gcgtgcgtga ggttcgccgc gtggcccgct cccgcaacca cgtgcagctg cgagcgcggc
     7561 agcgcgcgcg ccatctcctg tgcgtgcgcc agcgggatcg ccgcgtcagc gtcgccatgg
     7621 atcaccagcg ccgggacctt gatcgccgcg atcttgccgc tgatgtcatc gcgcgtcgcc
     7681 agcgtggaga agatctgctg caggttggcc ggtgaattct tgcgccactt cgctttccac
     7741 ttcggggcat cggcgtagcc cgcaccaagg atgatgtgct ctatggtcgc tgccatctcg
     7801 tcggacaaac cctgtgcggc ccaggtggca atcatctgct ggtacaccgg cagcgtggcc
     7861 ggatcttcgg gacgggcctg cgtgtcgatg aggacgagcc cgctggcgag tcccgggtgc
     7921 gtgagcgccg cgcgcagcga gagatagccg ccctgcgaca tgccggccag caccgcgcga
     7981 tcgatgccca ggtgcgtgag cagcgcggcc aggtcattgg ccgagtcgta gtaggagaac
     8041 gcttcccgcg catcgccggt gaggccgtgg ccgcgctcgt cccaggtgat cacgcggtac
     8101 ctgccgcgca gcgcagccac ctgcggcgcg aacatctcgc ggtccatcag cagtccgtgc
     8161 gagaagatga ccaccgggcc gcttccgccg gtgtcctcga agtggatctt ctgtccgttg
     8221 acgtcggcga tgggcatgcg aggtctcctc gtgcgtgcga gctatagacg actgtaccag
     8281 tggatgccgt gttcgtcggt gcggcgcagc gcctggccgc ggcgcagcag gtagttgaga
     8341 tgcgccagcg cttcgccggt ggccaggccg agcagctcgt cgccgatggg gcgcttgtac
     8401 agcgccgaga acaggtccac cacgcgccgc ggttcggcca gcgcctcgcg cacgcgcgcc
     8461 agcccgcgct cgtggccgcg ctgcaggtgc gcaaggcggc gatgcagtcc gcggaacggc
     8521 aggttgtgcg ccggcagcac cagcacgtcg tcgggcacca tgcgcgccac cagcgccagc
     8581 gaatcgagcc actcggccag cgggtcgccg tccggctcgg tgggaaacac cgacacgttg
     8641 gagctgatgc gcggcagcac ctggtcgccg gaaatgagca cgcgcaattc cgggcaatgc
     8701 aggcacaggt gttcgggcga gtggccgcgg ccgatcaccg cgcgccagtc gcgtccgccg
     8761 atgctgatga cgtcgccgtc gactacgcgg cggtagctgt cgggcagcgt gtacacaccc
     8821 ttgccgaagc cgccgaagcg cgtgcggtag tgctccagtg cgcgctcgct ccagccggcg
     8881 gcggcataga agcgcaccgc gtcggccggc gcctcgcggc cggtgtcggc caccagcacg
     8941 cggcaggtca ggtattccag ccgcgtcatc cacaggcggc agccgaagcg gcgggtgaac
     9001 cagccggcca tgccgacgtg gtccgggtgc atgtgcgtgc tgatcacgcg ggtgaccggc
     9061 aaaccctgca gcgggccggc catggcgctt tcccatgcag ccagcgtctg cgcgccctgc
     9121 atgccggtgt cgaccacggt ccagcggtcg ccgtcgcgca gcgcccacac gttgatgtga
     9181 tccagcgcaa agggcagcgg catgcgcagc cacagcacgc cgtcggccac ttccactgcg
     9241 tggccgggaa ccggcgcctg cgcccacggg taggccagcg gttccggcga cgcgtcgctc
     9301 tcttctggtc cgtccatggt gtctgctacg aaaaccgtag catggtcagg cgaggaagcc
     9361 gccgtcgatg gtcagcgtgg tgccggtgac gtagctggcc gcgtccgaca ccaggtagag
     9421 aatgccgggc gcaatctccg ccggctgcgc cacgcgcccg agcgggatga acggcaggaa
     9481 atgcttgagc atggcctcgt tcttggtcag cgccgaggcg aatttggtgt cggtaagtcc
     9541 gggcagcacg gcgttgacgc ggatcttctg cggcgcgcat tccttggcga aggccagcgt
     9601 catgttgatc accgccgcct tggtgatcga gtagatgccc tgcagcgggc cgggcttgat
     9661 gccgttgatc gaggcgatgt tgacgatggc gccaccgccg ttgttcttca tctgcttggc
     9721 cgccagcgcg gagagctcga agtaaccctt gatgttcacc tgcagggtct tgtcgaccat
     9781 gcccatgtcg gtgtcggcga tgtggcccat gtacgggttg gtcgcggcgt tgttgaccag
     9841 gatgtcgagg cggccgagct tgcccacgat ctcgccgagc aggtgcttga gcgcggcgga
     9901 gtctccctgg tgcgcggcga tggccgtggc cttgccgccg gtggcgatga tcgatgccgc
     9961 caccttgtcc aggtcttcct gcttgcggct ggacaccacg acatgcgcac cctgttcggc
    10021 cagcaggcgc gcggtagcct cgccgatgcc gcgcgaggcg ccggtgacca gcgcgacgcg
    10081 gccggtgaga tcaaaaagtt tggtggccat caatgtgctc cttctattgg attccgttat
    10141 aggagggggc cttgcgcgcg atggtgtgtg cgcatcgcgc gcaaggcgcg ctcctacaat
    10201 ttcgatttgt cgatgacgtt gcccgccacc tgcgacagca cggtgacgaa catgccgaag
    10261 gtctggaagc gcgggttctt cgtctgcccc atcttccagc ggtaatagat ctgctgcgcg
    10321 atgaccgcca gccggaacag gccgaacacg tagtagaagt cggtgttgga aatctcgcgc
    10381 ccggtcttct cgccgtagta gcgcacgatc tcctcgcgcg tcagcatgcc gggcagggtg
    10441 gagggctgca tgcgcagcgc ctgcatctgc ttgctgtcgc tgttctgcac ccagtaggcg
    10501 agcgacgagc ccaggtccat cagcggatcg cccagcgtgg ccatctccca gtcgagcacg
    10561 ccgatgatgc tctggtgatc ggacggcgac agcaccacgt tgtcgaagcg gtagtcgttg
    10621 tggatgatgc cgggccgcgg cgagtcggcg ggtttcttcg ccgccagcca ggccatgatg
    10681 ttctcgcagc gcggcacgtc gtcggtccag gccttgccga agcgttcgtt ccagccgttg
    10741 acctggcgct cgacgtaacc cgtcggcttg cccatgtcgg agagcccgag ctggtcatag
    10801 tcgagcgtgt gcagcctgat ctgcgtgtcg agcaggttga ggcacagctg gcgcgcagcc
    10861 tcgggcgtat aggaaattcc ttttggcgga tcgcgccgca ggatcacgcc ggtgatcttc
    10921 tccatcacgt agaaagtgga gccgatcagg ctatcatcct cgcagtaggc cagcgggcgc
    10981 gggcagggga aatgcggcga caggcgctgc atcacgcgga actcgcggcc catgtcgtgc
    11041 gcgctcttgg ccttggtgcc ggccggcggg cggcgcagga tcatctcgcg ctcgcccacg
    11101 cgcagcaggt aagtgacatt ggatgcgccg ccctggaact gctgcagcgt cgccgcgccg
    11161 cgcaggccgg ggatgtgctg ctgcaggaac ggcaggatca gtgccgggtc gaaattgttc
    11221 tcgtcgcgga cggcgccagc ctggtcgagt gtcgtgtcgg tcatgtggtg ctcagcggaa
    11281 ggtcagcagc gcggcatcgc cggccagttc cagtggcgtg atgttgttga agccggtcag
    11341 cgacaggccg ctgcggccga cccggaaggt gtgcaccgag gcgttgtaga tgcgccagtt
    11401 catgcggaag gtcatctcgt cggtcaggcc cagcgcctgc tgcagcgcca ccgcgatgac
    11461 gcccccggag gtgaagacca ccacctgctt gcgtccgggc gcggcgactt cgcgcaggcc
    11521 ggcaagcacg cgcgcacgga acctgctcca gccctccagc gcgatgccgc cgtccggctc
    11581 gcgccccgac agccacagcg cgatggcttt ctcgaagacc agctggaacg cgcgcgggtt
    11641 ggcgtacagc tccttgcgct cgatcgatat ggccggctcc tcgcgcgcca ccaccggcag
    11701 gtagccgcgc aggatgccgt cgaagtcgta ttcgttgaac gccgagtgcg tggtcaccgc
    11761 gctgctgcgc tgcatcgcct ccagcgcatg caggccggtg gtcttctgcc gcaccatgtc
    11821 gccgcagtac aggccgtcca tgtcgaagcc ggtgcgcttc cagtgctcgc ccagcacgaa
    11881 actctggcgc cgcccgtttt cggacagcgc gtcgtagctg gcggcgccga acgaggcctg
    11941 gccgtggcgg acgaggacta gcgtggtcaa gattcagccc atctgcagtc ggaagagggg
    12001 cgcaccttag cagcaaccac ggccgcgaaa aactcccgcg ccgcaacgct gcacggcaag
    12061 caaaattacc gcttcgacga agctatgcgg cgtcggccac ggctgccggt tcggccagcg
    12121 cccgcagttc gcgcgtcacc gcatcggcgc cgcgggcgga catgatgatg gtgtagtggt
    12181 tgacgtccgc gatcgtcgtg ctgctgaagt tggcgatgcg cgccgcccac ctgcgcacca
    12241 gcgcctcgct gtacagcggc ttgccgtcct gcagaccgcg cggcgcgcgc agcaggcgca
    12301 ccggcatttt cagctgcagc agcgagcgcg cgatgaggtc gtctcccatc atgctgcgcg
    12361 tatcgccgat caccgcgtcc ttgctggtgc tcgaacgcag ggcaggcgcc gtgccgacca
    12421 ggtcatggtc gaaatactgg accaggtcat ccgaccagtc ggaggccagc gcgggatgct
    12481 tgcgccagta ggccaggtat tcctcgcgcg aggcgaagct catgtcgagc
//
LOCUS       DASZZC010000059         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_712340,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000059 DASZZC010000000
VERSION     DASZZC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712340"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="hemC"
                     /locus_tag="VHE37_01510"
     CDS             complement(<1..696)
                     /gene="hemC"
                     /locus_tag="VHE37_01510"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HVT34233.1"
                     /translation="MKLRIATRESPLALWQAEHVRDRLQRAHAGLEVALVPMTTSGDQ
                     LLSAPLAAAGGKGLFVKELEQAMLEQRADIAVHSMKDVPARQPDGLTLAAFLHGEDPR
                     DAFVSNKHVNLDALPRGAVVGTSSLRRQAQLRALRPDVRIEVLRGNVGTRLRKLDEGH
                     YAAILLACAGLRRLGLEQRITQALDVTRFIPAIGQGVIGIECRSDDLATQALLQPLHD
                     ATSAARLAAERALN"
     gene            complement(746..1492)
                     /locus_tag="VHE37_01515"
     CDS             complement(746..1492)
                     /locus_tag="VHE37_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVT34234.1"
                     /translation="MRVVIVDDEPLARERLRRLLADFPGYEVVGEAGDGEAALDVIDE
                     EEPDLVLLDIRMGGMDGLQVSRQLQEMEVPPAVIFTTAYSEHALSAFDANAQAYLLKP
                     IRLEKLKEALMRVRKPTRAQKQQASAPEDGGKPKREFVLATTRDGLVRVPAEDIIYFL
                     ADQKYTTVCHLHGEVLIEESLKTLEEDFAPWFLRIHRKALVATRFIASLERERHGEQH
                     HWLRVKHAANPLPVSRRRLAEVRRFITEQS"
     gene            complement(1489..2616)
                     /locus_tag="VHE37_01520"
     CDS             complement(1489..2616)
                     /locus_tag="VHE37_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVT34235.1"
                     /translation="MKGLKGLKSSLSSMRRIRPSVAHRVNLIPDFCRAQGLVPLAFTM
                     ELVAIVLTLAGGATGTAAIDRLILLSIYLQWIGLCSAAALCRARRWLLIARPGVVFFV
                     CWGMIVLMVMVIADVAYFSAHQLRYGWLLPAETRVVFIGRHTCIAAIVALLLLRYYWT
                     RHEWQDQVRAEGESRYQALNARIRPHFLFNALNSLAALISIRPNEAETMVEDLSELFR
                     ASLEKRGQVGPLADEVGLCHAYLRIEKARLGDKLVTDWDVPDSLLAWPVPMLVVQPLV
                     ENAVHHGVSKMKDTGTIRISAREVDQRMVVEVENPLPPGESRDSHGNRIAVDNIAQRL
                     NLIYGDAARLELGRDQRLEGPVFRARLVLPREPKKDEGEHK"
     gene            2879..>4109
                     /gene="argH"
                     /locus_tag="VHE37_01525"
     CDS             2879..>4109
                     /gene="argH"
                     /locus_tag="VHE37_01525"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HVT34236.1"
                     /translation="MWSGRFAESTTELVARFSESVSFDQRLYRQDIAGSQAHARMLAK
                     VGVLSAAERDAIVRGLDQIRADIETGRFEWKSSLEDVHMNIEALLTQRIGEAGKKLHT
                     GRSRNDQVATDLRLYVRDAIDAIDALLARVAEALLALAESEADSLMPGFTHLQIAQPV
                     TFGHHLLAWREQVVRDRARFADARRRTNVLPLGSAALAGTVYPIDRAYTAKLLGFDAV
                     TENSLDAVSDRDFAIEFCAAASICMVHLSRWSEELILWSTPMFGFVSLPDRFCTGSSI
                     MPQKKNPDVPELVRGKTGRVIGDLNALLVLMKGQPLAYNRDNQEDKLPVFDALDTTLA
                     CLRVYAGLIETLNVRRERMARAAGEGYATATDLADYLVRKGVPFRDAHEIVGRAVRRG
                     LESGRDLAQLPLEELKAF"
BASE COUNT          669 a         1399 c         1368 g          673 t
ORIGIN      
        1 gttgagcgcg cgctcggccg ccagccgcgc ggcgctggtc gcatcgtgca gcggctgcag
       61 cagggcctgg gtggcaagat cgtcgctgcg gcactcgatg ccgatcacgc cctggccgat
      121 cgcggggatg aagcgggtga cgtcgagcgc ctgggtgatg cgctgctcca gccccagccg
      181 ccgcaggccg gcgcaggcca gcaggatggc cgcgtagtgc ccctcatcga gcttgcgcag
      241 gcgcgtgccc acattgccgc gcagcacctc gatgcgcacg tcgggccgca gcgcgcgcag
      301 ttgcgcctgc cggcgcaggc tcgaagtgcc gaccaccgcg ccgcgcggca gcgcatcgag
      361 gttgacgtgc ttgttggaga cgaaggcatc gcgcgggtct tcgccgtgca ggaaggcagc
      421 gagcgtcagc ccgtccggct ggcgcgcggg cacgtccttc atcgaatgca cggcgatgtc
      481 ggcgcgctgc tcgagcatgg cctgctcgag ctccttgacg aacagcccct tgccgccggc
      541 ggcggccagc ggcgcggaca gcagctggtc gccgctggtg gtcatcggca ccagcgccac
      601 ttcgaggccg gcgtgcgcac gctgcagacg atcgcgcacg tgctcggcct gccacagggc
      661 cagcggggac tcgcgggtgg cgatgcgcag tttcactcgc gcattgtagg cgcgcggcga
      721 cagcgcaggt acggcgtcag cgcggtcagc tctgctcagt gatgaagcgc cgcacctcgg
      781 ccagccgccg gcgcgacacc ggcaacgggt tggccgcgtg cttcacccgc agccagtggt
      841 gctgctcgcc gtggcgctcg cgctcgagcg aggcgatgaa gcgggtggcc accagcgcct
      901 tgcgatggat gcgcaggaac cacggcgcga aatcctcttc cagcgtcttc agcgattcct
      961 cgatcaacac ttcgccgtgc aggtggcaca cggtggtgta cttctggtcg gccaggaaat
     1021 agatgatgtc ctcggccggc acgcgcacca gtccgtcacg cgtagtcgct agcacgaatt
     1081 cacgcttggg cttgccgccg tcttccggcg cgctggcctg ctgcttctgc gcgcgcgtgg
     1141 gcttgcgcac gcgcatcagc gcttccttga gtttctccag gcggatcggc ttgagcaggt
     1201 aggcctgcgc gttggcgtcg aacgcgctca aggcatgctc ggagtacgcg gtggtgaaga
     1261 tcaccgccgg gggcacttcc atctcctgca gctggcgcga cacctgcagc ccgtccatgc
     1321 cgcccatgcg gatgtcgagc agcaccaggt cgggctcttc ctcgtcgatg acgtcgagcg
     1381 cggcttcgcc gtcgcccgcc tcgcccacca cctcgtagcc cgggaaatcc gccagcagcc
     1441 ggcgcaggcg ctcacgcgcc agcggctcgt cgtccacgat caccactctc atttgtgctc
     1501 tccctcgtct ttcttgggtt ccctgggcaa caccagacgc gcgcggaata ccgggccctc
     1561 cagccgctgg tcgcggccca gttccagtcg cgcagcatca ccataaataa ggttcagccg
     1621 ttgggcaatg ttgtcgaccg cgatgcggtt gccgtggctg tcgcgcgact cgcccggcgg
     1681 cagcgggttt tccacttcca ccaccatgcg ctggtccacc tcgcgcgcgg aaatccggat
     1741 ggtgccggtg tccttcatct tcgacacgcc atgatgcacc gcgttctcca ccagcggctg
     1801 caccaccagc atcggcaccg gccaggctag cagcgaatcg ggcacgtccc agtcggtgac
     1861 cagcttgtcg cccagccgcg ccttctcgat gcgcaggtag gcatggcaca ggccgacttc
     1921 atccgccagc gggccgacct gtccgcgctt ttccagcgac gcgcggaaca gttcggacag
     1981 gtcctcgacc atggtttccg cctcgttcgg gcggatgctg atcagcgccg ccaggctgtt
     2041 gagcgcgttg aacaggaaat gcggacggat gcgcgcgttg agcgcctggt agcggctctc
     2101 gccttccgcg cgcacctggt cctgccactc gtggcgcgtc cagtagtagc gcagcagcag
     2161 caaggcaacg atggcggcga tgcaggtgtg gcggccgatg aacaccacgc gcgtctcggc
     2221 cggcagcagc cagccatagc gcagctggtg cgccgagaaa taggcgacgt cggcgatgac
     2281 catcaccatc agcacgatca tgccccagca gacgaagaac accacgccgg ggcgcgcgat
     2341 cagcagccag cggcgcgcgc ggcagagcgc ggcggcagag cacaggccga tccactgcag
     2401 gtagatggac agcaggatca gccggtcgat cgccgcggta ccggtggcgc cgccggccag
     2461 cgtcagcaca atcgccacca gctccatggt gaatgccagc ggcaccaggc cttgcgcgcg
     2521 gcagaaatcc gggatgaggt tgacgcgatg cgcgacgctc gggcggatgc gccgcatgct
     2581 cgacaacgac gacttcaggc ccttcaatcc tttcattgct ggtccgtgag tgcttttttg
     2641 ccctgaccgc accggcatct tagctgttat taaacattaa ttaattatta aattacacta
     2701 ttttttgtaa caggctatcc tgccttgcgg tgcagggcaa aacaggcgcg cgaagtcgcg
     2761 tcactataat gagcgcgccc catcccgcgt gtcccagcgt aactttatgt ccaagtccgg
     2821 ctccagaaaa aaaagtgcgg ccgccgcgcc gtccgcgtca gcgcaaggga agaacgccat
     2881 gtggagcggg cgctttgccg agtccaccac cgagctggtg gcgcgctttt ccgagtcggt
     2941 gagtttcgac cagcgcctgt accggcagga catcgccggc agccaggcgc acgcgcgcat
     3001 gctggccaag gtgggcgtgc tcagcgccgc cgagcgcgac gccatcgtgc gcggcctcga
     3061 tcagatccgc gccgacatcg agaccggcag gttcgagtgg aagagctcgc tggaagacgt
     3121 acacatgaac atcgaggcgc tgctgaccca gcgcatcggc gaggccggca agaagctgca
     3181 caccgggcgc tcgcgcaacg accaggtggc caccgatctg cgcctgtacg tgcgcgatgc
     3241 catcgacgcc atcgatgcgc tgctggcgcg cgtggccgag gcgctgctgg cgctggcgga
     3301 aagcgaggcg gacagcctca tgcctggctt cacccacctg cagatcgcgc agccggtgac
     3361 cttcggccat cacctgctgg cgtggcgcga acaggtcgtg cgcgaccgcg cgcgcttcgc
     3421 cgacgcccgt cgccgcacca acgtgctgcc gctgggcagc gcggcgctgg ccggcaccgt
     3481 gtacccgatc gaccgcgcct acacggcgaa gctgctcggc ttcgacgccg tgacggagaa
     3541 ctcgctggat gcggtgagcg accgcgactt cgccatcgag ttctgcgcgg ccgcgtcgat
     3601 ctgtatggtg cacctgtcgc gctggagcga ggaactgatc ctgtggtcga cgccgatgtt
     3661 cggcttcgtc tcgctgcccg accgcttctg caccggcagc agcatcatgc cgcagaagaa
     3721 aaaccccgac gtgccggaac tggtgcgcgg caagaccggc cgggtgatcg gcgatctcaa
     3781 cgcgctgctg gtcctgatga agggtcagcc gctggcctac aaccgcgaca accaggaaga
     3841 caagctgccg gtgttcgatg cgctcgacac cacgcttgcg tgtctgcgcg tgtacgcggg
     3901 cttgatcgag actctcaacg tacgccggga gcgcatggcg cgtgcggctg gcgagggcta
     3961 tgccacggcg accgaccttg ccgattacct agtgcgcaag ggcgtgccat ttcgcgacgc
     4021 ccatgaaatc gtcggtcgtg cggtgcggcg cggccttgaa agcgggcgcg acctggctca
     4081 gctcccgctc gaggagctca aggcgttcg
//
LOCUS       DASZZC010000060         8506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_455266,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000060 DASZZC010000000
VERSION     DASZZC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455266"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..747
                     /locus_tag="VHE37_01530"
     CDS             43..747
                     /locus_tag="VHE37_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34237.1"
                     /translation="MTTSRLKSRTKVTPLPRAKRGYAGLSSGEQDLARRRRLLDTALE
                     LFTRQGYARTPIEQLCVESRVTSRYFYELFESREALLRALFEEIIDDARAAVIAAIAQ
                     PGLTAEARISQAVTAFIRSYVQDARRARLCVVEAVGISEGLEKRRREVIHEFASVIRG
                     YADALAKASVIPKRNYHLFSVSMVGSINELLVEWLTAARPPSIEAVTQEIQLQFRVMV
                     LGGQALLNGSSREAAR"
     gene            744..3227
                     /locus_tag="VHE37_01535"
     CDS             744..3227
                     /locus_tag="VHE37_01535"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HVT34238.1"
                     /translation="MSRRSISANFCRHAATVGLLAVSMCLDGCSRSGKPVDAINVTAT
                     DVLALVNPYVGTQSGAADFGTGGGAGNTFPGATIPFGMVQLSPDTSPSTDNFAGGYTY
                     SDSLIKGFSLTHISGAGCAIYQDVPFLPVASEVTEAPVSVGSAGFSSQYLAAFTHDHE
                     AASPGYYQVRLNPQGAAPIDVDLTATTRGGMMRMKYSTGSPANLLINPGGSAMANALA
                     QVHIDPQLREVSGQSSSGRFCYQDNRYTVYFVAQFDHPFKSWAVWRKLVFTRNGTDAS
                     DSSPLPLNYGPVPGGPSSIPGNPSGTAQAGAVLSFDTQDDQPVVMRVAISYVSTVNAR
                     QNLERELAGLAFDEVRSGAQSTWRKELSRVQVEGGGPAWRRIFYTALYHSLLSPNVFS
                     DANGDYLGMDNQVHSTSRTAQYQNFSGWDIYRSQVPLLALLDPARTSDMVQSLVEDAN
                     QNGWLPKWSYANQQTDTMVGDPADLIIAGAYAFGARGFDTSSALNAMMKGATSSGISG
                     NPASLTNGYVERQALSTYDSLGYVPHDLDTPLGGIGYVASSEYVWGSAATTLEYALAD
                     FGIAQFADAIGQSGQVASIRARSANWRNLVNPGTEAIEPRLAVGAFVPRYDASGGDGF
                     VEGNGAQYSWFVPHDIAGLIEATGGQDQASARLDGFFSQINAGPKAPYAYLGNEPTLF
                     TPWIYAWLHQPSKTSAIVRQALTTLYDDTAKGMPGNDDLGTMSAWWVLSSLGLNPGIP
                     GTNVLLINAPLFDSATLDLPSGRVRITREAADGNGSIQSAMLGAEPLDRAWLHASELT
                     SKGNLSFVLGTAESSWATAMGSEPPSNSR"
     gene            complement(3381..4661)
                     /locus_tag="VHE37_01540"
     CDS             complement(3381..4661)
                     /locus_tag="VHE37_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HVT34239.1"
                     /translation="MADFGNGRQTHVSVQASTSILVGVVGLFFIWGGITSLNDVLIPK
                     LRDLFQLNYAQAMLIQFAFFTAYAVVSLPAGGLISRLGYGRGIVAGLSIMGIGCVLFV
                     PAASLASYALFLGGLFILAGGITILQVAANPLIANIGEPGSGHSRLTLAQAFNSLGTT
                     LMPYIGAQLILGDVAHASPANLSAQQLMDFQAREAAVVGHGYLAIAGICFLVAAAFWF
                     WRRALGQFSDGDSAASFSGSLALLRSRPRLAFGVLGIFLYVGAEVTIGSVLVNYLMLP
                     STLGLSQRAAGEHLPYYWGGAMVGRFIGAVLLRRIRAGVLLSAVAMGAVCLVLSSAAS
                     QGALAAWALLAVGLMNAIMFPTIFSLAVENMGPDTPRASGLLCMAIVGGAVVPLFSGF
                     VADASSLAVALTVPAACYLVIAAFGWSARKPAQA"
     gene            complement(4689..5951)
                     /locus_tag="VHE37_01545"
     CDS             complement(4689..5951)
                     /locus_tag="VHE37_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVT34240.1"
                     /translation="MKRGARIALAVVVALGAWVGWAALPPPASRVEPVKPLPADFIWG
                     VSSSAFQSEGGRVDSNIQRRNDASPQEDRYGNAVDFRHRYREDIALAKQLGINTYRMG
                     IDWARVEPQKGKIDEAELAFYDDVILAMKQAGIAPLITLDHFVHPGWVADQGGWANPQ
                     TTADFVHYASLMAQRYHADVHHWITFNEAAFFMPAEIKMHQLDRAGALRVRDNMASAH
                     RQVYDLIHALDAHAMVTSNIVWVGDHFGAWALRTLTNWLFLDQVEDKCDVIAIDYYSA
                     DVLEVAKVGKHWMWPPQPAGLYRALMMLKDRYPSKPVLIAETGMATENGKPRPDGIRR
                     EDVLRDSIYWTQRARDDGANVIGYMVWSLTDNFEWGSYTPRFGLYTVNALTDPQLARI
                     PTAAVPAYQAAIRAGGMPADYRPALGQK"
     gene            complement(6012..7427)
                     /locus_tag="VHE37_01550"
     CDS             complement(6012..7427)
                     /locus_tag="VHE37_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34241.1"
                     /translation="MKHHLRDAWVLLLPAMIGLAACGHSSAPPDQAAAAAVDTGITPP
                     ATPKAACGPGSRPESGLQGRLSQADIDSGLALAGITCNTELVGAYVDTSHLATVGGWK
                     VEHYADHSGHECAYYDATDIFPLGVFDNAVGVNVLDMTDPAHPAFIKHLVTPAMLSPH
                     ESLVISEARGLLVAVLGNPAFGPGVVDVYDVSQDCRDPQLQSIAPLGTFGHESGIAPD
                     GRTFFSASPSTPTLTAVDISDPKHPAPLWIGDMTSHGLSISADGNRAYVADTSGKVKI
                     MDISQIQSRAPSPRVPEVSEVTWDSVSIPQNAIPVTIKGKPFLVEIDEFGAGSKVGAG
                     RIIDIADETQPKVISNLRLEVHQPENFAAIANDPGASSTSYGGYAAHYCNVPTRVDPP
                     IVACSMILSGLRIFDIRDPYHPREVAYFNAPLDPSTLSSGPASNAAYSQPAFVPERKE
                     VWYSDSNSGFYAVRVTNGAWP"
     gene            complement(7627..8202)
                     /locus_tag="VHE37_01555"
     CDS             complement(7627..8202)
                     /locus_tag="VHE37_01555"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVT34242.1"
                     /translation="MSPRDAIIALWALWVVSWIAAAAWGSRTDKRPGIGTEIRYRIPM
                     LIGALFMFVPAHGYEGALRVWHIGWMGAWISVGCLATGIVFAWWARIHLGRLWSGSIT
                     RKTDHRVVDSGPYALVRHPIYTGLLFSLLATTAAKGTWLAIAGFALLLLGIWMKARFE
                     EQWLGQELDAGAYASYRQRVPMLLPFGPRGG"
     gene            complement(8213..>8506)
                     /locus_tag="VHE37_01560"
     CDS             complement(8213..>8506)
                     /locus_tag="VHE37_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVT34243.1"
                     /translation="ATFHGGGLATDKPDSPHLLIPQMKAQFLIAIAANDDAKDPAAKD
                     TLRTAFAQAKLPAEIEVYAGTQHGWCPTDSPVYDHDAAEKAWSRMLALFQHAL"
BASE COUNT         1497 a         2728 c         2783 g         1498 t
ORIGIN      
        1 gatatgggtt agtatgacaa gaaatcttgt cagattaaag ccatgacgac ttcccggctc
       61 aaaagtcgca cgaaagtcac cccgctaccg cgagcaaaac gcggctatgc cggcctgtcg
      121 tccggtgagc aagacctcgc caggcgacgg cggctcctgg ataccgcgct cgaattgttt
      181 acgcgccagg gctatgcccg gacgcccatc gagcagctct gcgtggagtc gcgtgtcacc
      241 tcacgctatt tctacgaact gttcgaaagc cgtgaagccc tgttgcgcgc gctctttgag
      301 gaaatcatcg atgacgcgcg cgccgccgtg attgccgcca tcgcccagcc tggtctgacg
      361 gccgaggccc gcatcagcca ggcggtgacg gcgttcatcc gatcgtacgt acaggatgcc
      421 cggcgtgcgc gcctgtgcgt cgttgaagcg gtaggaatca gcgaagggct ggagaagagg
      481 cgccgcgagg tcatccacga atttgcttcg gtgatccgcg gctatgccga tgccttggcc
      541 aaggccagcg tgatccccaa acgcaactac catctcttca gtgtctcaat ggtcggctcc
      601 atcaatgaac tactggtcga atggctgacc gctgcgcgtc cgccctccat cgaggctgtc
      661 acccaggaaa tccagcttca gttcagggtc atggttcttg gcggccaggc cttgctcaat
      721 ggatcgagcc gcgaggctgc caggtgagcc ggcgttccat cagcgcgaat ttctgccgcc
      781 atgcagccac agtgggtttg ctggccgtgt cgatgtgcct cgacggatgc agcaggtccg
      841 gcaagccggt tgatgcaatc aacgtgactg cgacggacgt tcttgccttg gtgaatccgt
      901 acgtcggcac ccaatccggc gcggcggatt ttggtactgg aggcggggcg gggaatacat
      961 ttcccggcgc caccattccg tttggaatgg tgcagctttc tcccgatacc tctccatcaa
     1021 ccgacaattt cgccggcggc tacacctatt cggacagctt gatcaaggga ttcagcctca
     1081 cgcacatctc gggtgccggt tgtgccatct accaggatgt gccgtttctt cccgtcgcct
     1141 ccgaggtgac ggaggcgccg gtgtccgttg gttccgccgg attttcttcg cagtacctgg
     1201 cagcgttcac gcatgatcat gaagcggcca gccccggcta ctaccaggtg cggctcaatc
     1261 ctcaaggggc tgcgcccatc gatgtggacc tgacggcgac cacgcgtggc gggatgatgc
     1321 gcatgaagta ttccaccggg tctcccgcaa atctgctgat caatccaggg gggagcgcca
     1381 tggccaatgc gctggcccag gttcacatcg acccgcagct gcgcgaagtc agcggccagt
     1441 cgtccagcgg tcgcttctgc taccaggaca atcgttacac cgtctacttc gttgcgcagt
     1501 tcgatcaccc tttcaaatcc tgggcggtgt ggcgcaagct ggttttcacc aggaacggca
     1561 cggatgcatc tgacagttca cccctgccgc tgaactatgg ccctgtcccg ggtggccctt
     1621 catcgattcc aggcaatccc agcggcacag cacaggccgg cgccgtgctg agcttcgaca
     1681 cgcaggatga tcagccagtg gttatgcgcg tcgctatctc gtacgtaagc acggtcaacg
     1741 cccgccaaaa cctggaaagg gaattggctg ggctggcctt cgacgaggtg cgctcagggg
     1801 cacaatccac ctggcgcaag gaattgtccc gggtgcaggt cgaggggggc ggtcccgcgt
     1861 ggcgccggat tttctacacc gccctctacc actcgctgct ctcgcccaac gtcttcagcg
     1921 atgcgaacgg cgactacctg ggcatggaca accaggtgca ttcgacatcg cgcacagcgc
     1981 agtatcagaa cttctccggg tgggatatct atcgctccca ggtcccgttg ctcgcgctgc
     2041 tggatcccgc gcgcaccagc gacatggtgc agtcgctggt cgaagacgcc aaccagaacg
     2101 ggtggctgcc caagtggtcg tatgccaatc agcagacgga cacgatggtc ggcgatcccg
     2161 ccgatctcat cattgcgggg gcctatgcct ttggtgcacg agggtttgat acgtcctcgg
     2221 cactgaatgc gatgatgaag ggagcgacgt catcgggcat ctcgggcaat cccgccagcc
     2281 tcaccaacgg ctacgtcgaa cggcaagcac tgtcgaccta cgacagcctg ggctatgtac
     2341 cccacgacct ggacacgccg ctgggcggca tcggatacgt cgccagctct gaatatgtct
     2401 ggggcagcgc cgcgacgacc cttgaatacg cgcttgccga cttcggtatt gcccagttcg
     2461 ctgacgccat cggtcaatca gggcaggtag cttccatacg cgcgcgctca gccaactggc
     2521 gcaatctcgt caatccaggg accgaggcca ttgagccccg gcttgccgtc ggcgcattcg
     2581 tcccgcgata tgacgcgagc ggaggcgatg ggttcgtgga gggtaacggc gcgcagtaca
     2641 gctggtttgt gcctcacgac atcgctggac ttatcgaggc caccggcggg caggaccagg
     2701 catccgcgcg tctcgatggt ttcttcagcc agatcaacgc cggccccaag gctccctacg
     2761 catatctcgg taacgagccg acactcttca caccctggat ctatgcgtgg ctgcatcaac
     2821 cgtccaagac cagtgcaata gtgcgccagg cgctcaccac gctgtatgac gacaccgcca
     2881 aaggcatgcc cggcaatgac gatttgggaa ccatgtcggc ctggtgggtt ctgagttccc
     2941 tgggcctcaa tccgggcatt cctggtacga atgtgctgtt gatcaatgcg ccgctgttcg
     3001 acagcgcgac cctggacctt cccagcggcc gggtccgcat caccagggag gcggcggatg
     3061 gcaacgggtc gatccagtcc gccatgctcg gggcggaacc gctggatcgc gcctggctcc
     3121 atgcgtcaga gttgacgtcg aaagggaatc tcagcttcgt gctgggtaca gcagagtcct
     3181 catgggcgac cgccatgggc tcggaaccgc cgtcgaactc ccgataggag ttgtccttgc
     3241 gctgcgcgtg aattgcagtt gtcctcgacc atggaccact tgagaggctg cgtgtgtggc
     3301 aatcgcacca tggcgaagcc attttcacga tcaaccttaa gcgaacgtcc tgctcatgcc
     3361 aatttcgagc aagcaaccgc ttacgcttgc gccggcttgc gcgccgacca gccaaacgcg
     3421 gcgatcacca ggtagcaggc cgcggggacg gtcagcgcca cggccaggct cgaggcatcg
     3481 gcgacgaatc ccgagaacag cggaaccacg gcgccgccga caatggccat gcacagcagg
     3541 ccagacgcgc gcggcgtgtc ggggcccatg ttctcgactg caaggctgaa gatggtcggg
     3601 aacatgatcg cgttcatcag ccccaccgcg agcagggccc atgcggccag cgcaccttgc
     3661 gaagcagccg agctgagtac caggcagacg gcgcccatgg ccacggccga cagcagcacg
     3721 ccggcgcgga tccggcgcag cagaaccgcg ccgatgaagc gtccgaccat cgcgccgccc
     3781 cagtagtacg gcaggtgttc gccggccgcg cgctggctga gccccagcgt cgacggcagc
     3841 atcaggtagt tgaccagcac gctgccgatg gttacctcgg cgcctacgta aagaaagatg
     3901 ccgagcacgc cgaaggccag ccgtggccgg ctgcgcagca gcgcgaggct gccggagaac
     3961 gatgcggcgg aatcgccatc gctgaactgg cccaacgcgc gccgccagaa ccagaacgcg
     4021 gcggcgacga gaaaacagat tcctgcgatg gccaggtagc catggccgac gaccgccgct
     4081 tcgcgcgcct ggaaatccat caactgttgc gccgacaggt ttgccgggga agcgtgggcc
     4141 acgtcgccta ggatcagttg cgcgccgatg tagggcatca gcgtggtgcc cagcgagttg
     4201 aacgcctgcg ccagcgtcag ccggctgtgg ccggagccgg gttcgccgat gttggcgatc
     4261 agcggattgg ccgccacctg caggatggtg atgccgcccg cgaggatgaa cagcccgccg
     4321 agaaacagcg cgtaactggc cagcgaggcc gccggcacga acagcacgca gccgatgccc
     4381 atgatggaca gcccggcgac gatgccgcgt ccgtagccca gccgcgaaat cagtccaccg
     4441 gccggcagcg agaccacggc gtaggcggtg aagaaggcga actggatcag catggcctgc
     4501 gcgtagttca gctggaacag gtcgcgcagc ttgggaatga gcacatcatt gaggctggtg
     4561 atgccgcccc agatgaagaa cagccccacc acgcccacca atattgatgt ggacgcctgg
     4621 acgctgacat gcgtctgcct gccattgccg aaatcagcca tgaatccccc aacgcgtgag
     4681 tgccgcgatc acttctgtcc gagcgccggg cgatagtccg ccggcatgcc gccggcgcgt
     4741 attgccgcct ggtacgccgg caccgcggcg gtggggatgc gcgcgagctg cggatcggtc
     4801 agcgcattca ccgtgtacag gccgaaacgc ggcgtgtagc tgccccattc aaagttgtcg
     4861 gtcagcgacc acaccatgta gccgatcacg ttggcgccgt cgtcgcgtgc gcgctgtgtc
     4921 cagtagatgc tgtcgcgcag cacgtcctcg cgcctgatgc cgtcgggccg cggcttgccg
     4981 ttctcggtcg ccatgccggt ttctgcgatc agcacgggtt tggacggata gcggtccttg
     5041 agcatcatca gcgcgcggta gaggccggcc ggctgcggcg gccacatcca gtgcttgccg
     5101 accttcgcca cctcgagcac gtcggccgag tagtagtcga tggcgatgac gtcgcacttg
     5161 tcctcgacct ggtcgaggaa cagccagttg gtcagcgtgc gcagggccca ggcgccaaag
     5221 tggtcgccga cccagacgat gttgctggtg accatggcat gcgcgtcgag cgcgtggatc
     5281 aggtcgtaga cctgccggtg cgcgctggcc atgttgtcgc gcacgcgcag cgcgccggca
     5341 cggtccagct ggtgcatctt gatctcggcg ggcatgaaga acgccgcttc attgaaggtg
     5401 atccagtggt gcacgtcggc gtggtaacgc tgcgccatga ggctggcgta gtgcacgaag
     5461 tcggcggtgg tctgcggatt ggcccagccg ccctggtccg ccacccaacc tggatgcacg
     5521 aaatggtcga gcgtgatcag cggcgcgata ccggcctgct tcatcgccag gatgacgtcg
     5581 tcgtagaacg ccagctcggc ttcgtcgatc ttgcccttct gcggctccac gcgcgcccag
     5641 tcgatgccca tgcgataggt gttgatgccc aactgtttgg ccagtgcgat gtcttcgcgg
     5701 tagcggtgac ggaagtccac cgcgttgccg tagcggtctt cctgcgggct ggcgtcgttg
     5761 cggcgctgga tgttggagtc cacccgtccg ccttcgctct ggaacgcgga cgatgacacg
     5821 ccccagatga aatccgccgg cagcggcttg accggctcca cccggctggc cggtggcggc
     5881 agcgccgccc agcccaccca tgcgccgagc gcaaccacca cggccaatgc aatcctggcg
     5941 cctcgcttca tctgctgctc cccatcttgt tggtcgatta tcagcgacct ggcgaggcgc
     6001 cgggcctctc cttacggcca ggccccgttg gtgacgcgca ctgcatagaa gccgctgttg
     6061 gagtcgctgt accagacttc cttgcgctcg ggcacgaagg ccggctgcga ataggccgca
     6121 ttcgatgccg gcccggacga cagcgtggac ggatcgagcg gtgcgttgaa gtaagccact
     6181 tcgcgcgggt gatacggatc gcggatgtcg aagatgcgca ggcccgagag gatcatgctg
     6241 caggcgacga tgggcggatc gacccgcgtc ggcacgttgc agtagtgcgc cgcgtagccg
     6301 ccatacgagg tgctcgacgc gcccgggtca ttggcgatgg cggcgaagtt ctccggctgg
     6361 tgcacttcca gccgcaggtt ggagatcacc ttcggctgcg tttcgtcggc aatgtcgatg
     6421 atgcggccgg cgccgacttt cgagcccgcg ccgaactcgt cgatctcgac caggaacggt
     6481 ttgcccttga tggtgacggg aatggcgttt tgcggaatgc tcaccgagtc ccaggtcact
     6541 tcggaaactt ccggcaccct gggcgaaggc gcgcgcgact ggatctgcga gatgtccatg
     6601 atcttcacct tgcccgaggt gtcggccacg taggcgcggt tgccgtcagc gctgatcgac
     6661 agcccgtgcg aggtcatgtc gccgatccac agcggcgccg gatgcttggg gtcgctgatg
     6721 tccactgcgg tcagcgtggg cgtggacggc gaggccgaga agaaggtgcg accgtccggc
     6781 gcgatgccgc tctcgtggcc gaaggtgccc agcggcgcga tcgattgcag ctgtggatcg
     6841 cggcagtcct gcgaaacatc gtagacatcg accacgcccg gcccgaatgc cggattgccg
     6901 agcacggcga ccagcagccc gcgtgcttcg ctgatcacca gcgactcgtg cggcgacagc
     6961 atggccggcg tcaccaggtg cttgatgaag gccgggtgcg cgggatcggt catgtcgagc
     7021 acgttgacgc cgacggcgtt gtcgaacacg ccgagcggga agatatcggt ggcatcgtag
     7081 taggcgcatt catgcccgct gtggtcggca tagtgctcca ccttccagcc gcccaccgtc
     7141 gccaggtggc tggtgtcgac gtaggcgcca accagttcgg tgttgcaggt gatgccggcc
     7201 agcgcgaggc cgctgtcgat gtcggcctgc gacaggcgtc cctgcagccc ggattccggg
     7261 cgcgagccgg gaccgcatgc ggccttcgga gtagccggcg gtgtgatgcc ggtgtcgacc
     7321 gcagcggcgg cagcctggtc gggcggcgcg gacgagtggc cgcaggcggc gaggccgatc
     7381 atcgcgggca acagcaacac ccatgcgtca cgcaggtgat gcttcatcag tctatctcct
     7441 cgtcgcggcg cggcgcatgg atctgcgctg cctttattga cggctcaccg ccgtatccgc
     7501 gaccgggcgc gcctgatcgc ggatcgacgt gttatggatc gagtgtgccc ccgcgcgcga
     7561 tgcccaccta tcacacctgt gacagggccg gagctgcagg aaatttcgcc ggcgtgccgg
     7621 cgggtatcaa ccgcccctgg ggccgaacgg cagcagcatg ggcacgcgct ggcgatagct
     7681 ggcgtaggcg ccggcatcta gttcctggcc cagccattgt tcctcgaagc gcgctttcat
     7741 ccagatgccg agcaggagca gggcgaagcc cgcgatggcc agccaggtgc ccttggccgc
     7801 ggtggtggcg agcagcgaaa acagcaggcc ggtgtagatc ggatgacgca ccagtgcgta
     7861 cggtccgctg tcgaccacgc gatggtcagt cttgcgcgtg atgctgcccg accacaggcg
     7921 gcccaggtgg atgcgcgccc accaggcgaa gacgatgccg gtggcgaggc agccgacgct
     7981 gatccacgcg cccatccagc cgatgtgcca gacgcgcagc gcgccctcgt agccatgcgc
     8041 cggcacgaac atgaacagcg cgccgatcag catcgggatg cggtagcgga tctcggtgcc
     8101 gatgcccgga cgcttgtcgg tgcggctgcc ccacgccgct gcggcgatcc acgacacaac
     8161 ccacagggcc cacagggcga tgatggcgtc gcgcgggctc atggccgatc gctcacaacg
     8221 cgtgctggaa cagcgcgagc atgcggctcc aggccttctc tgccgcgtcg tggtcgtaca
     8281 ccggcgaatc ggtcgggcac cagccgtgct gggtgccggc gtagacctcg atctctgcgg
     8341 gcagcttggc ctgcgcgaag gccgtgcgca gcgtgtcctt ggccgccgga tccttggcgt
     8401 catcgttggc ggcgatggcg atgaggaact gcgccttcat ctgcgggatg agcaggtgcg
     8461 gactgtcggg tttgtccgta gccagtccgc cgccgtgaaa ggtggc
//
LOCUS       DASZZC010000061         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_324906,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000061 DASZZC010000000
VERSION     DASZZC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324906"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1380)
                     /locus_tag="VHE37_01565"
     CDS             complement(40..1380)
                     /locus_tag="VHE37_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVT34244.1"
                     /translation="MLKKILATSLLLAAPVAQAADDHAAGAVHEHRLANGLQLLVWPS
                     HDIPSVAVYTFFRVGSRNERPGITGLSHFFEHMMFNGTKTRPPGTFDRLLEAAGGNNN
                     AYTTEDVTVYQDWIPKSALSLAFDLESDRMRNLDFDPKVVESERGVVYSERRLRVDND
                     NAAALEELVQASAFVAHPYQIPVIGWPSDIESWKRSDLQHYYKTYYAPNNAVMVVAGD
                     VQPDAVFALADRYYGTIKPSQLPDPVRTTEPPQAGERRVVLKRPGQAPLLQLAYHAPR
                     AADDAVPALELLLAILAEGDSSRLYRLLVEDTRTAVSVQGQLQAGFDPGLAWLNVTLP
                     PGGDVARVQQQIDEALARVVRDGVTDTELAKARNLKLAQFWKSLSTIAGKAQALGHCE
                     VLGGGRARLFETPDRYAAVTGDQVRRIAAQVFRADNRTVGVLQPQAQAAPGDAP"
     gene            complement(1390..>2647)
                     /locus_tag="VHE37_01570"
     CDS             complement(1390..>2647)
                     /locus_tag="VHE37_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT34245.1"
                     /translation="AYPRYEALLTSIAEHLEPVLMDTAPELLPLPASWRRRGWLARLR
                     DLLRARRLYRALADLGEDIPQALEILTGAATPILDRWFESPELKGTLATDAIIGTFQP
                     PSAPGTAYVLLHHVMGMAGGVRGVWGYVEGGMGALTQALARSAQAAGVEIRCNAAVEE
                     ILVEGGRAVGVRLQGGEVLRAFAVASSATAQVTFGALLDARHLPGPFREAVARIDYSS
                     ASMKINLALSELPDFRCCPGTALGPQHRGTIHINAMPEQIERAYDDAKYGVPSRRPVI
                     EMVIPTSVDATLAPPGHHIANLFVQYAPYRLAQGKWDEIGQSYADQVIAEVTRHAPNF
                     ARSVLHQQVLSPLDLERRFALTGGNIFQGAMPLHQLFSLRPVPGWSDYRTPVRGLYLC
                     GAAAHPGGGVMGACGRNAAREMLRDF"
BASE COUNT          392 a          934 c          910 g          411 t
ORIGIN      
        1 acaccgaggc cagcgtccac atgagcctgc gcgcgatcgt catggcgcgt ctcccggggc
       61 cgcttgcgcc tgcggctgca gcacgcccac tgtgcggttg tcggcgcgga atacctgcgc
      121 ggcgatgcgg cggacctggt cgccggtcac cgccgcgtag cgatcaggtg tctcgaacaa
      181 gcgtgcgcgc ccgccgccga gcacctcgca atggcccagc gcctgcgcct tgccggcgat
      241 ggtcgacagg ctcttccaga actgcgcgag cttgaggttg cgcgccttgg ccagctcggt
      301 gtcggtgacg ccgtcgcgca ccacccgcgc cagggcctcg tcgatctgct gctgcacgcg
      361 cgcaacatca ccacccggtg gcaaggtcac gttgagccag gccaggccag gatcgaagcc
      421 ggcttgcagc tgcccctgca ccgacaccgc ggtgcgcgtg tcctccacca gcagccggta
      481 caggcgcgag gaatcgccct cggcgaggat ggccagcagc agttccagcg cgggcacggc
      541 gtcatcggct gcgcgcggcg cgtggtacgc cagctgcaac agcggcgcct ggcccggccg
      601 cttcagcacg acacggcgct cgccggcctg cggcggctcg gtggtgcgca ccgggtcggg
      661 cagctgcgag ggcttgatcg ttccgtagta tcggtcggcc agcgcgaaca cggcatcggg
      721 ctgcacgtcg ccggccacca ccatcaccgc gttgttcggc gcgtagtagg tcttgtagta
      781 atgctgcagg tcgctgcgct tccagctctc gatgtccgac ggccagccga tcaccgggat
      841 ctggtacgga tgcgcgacaa acgcgctggc ctgcaccagc tcctccagcg ccgcggcgtt
      901 gtcgttgtcc acgcgcaggc ggcgctcgga ataaaccacg ccgcgctcgc tctccaccac
      961 cttcggatcg aaatcgagat tgcgcatgcg gtcgctttcg aggtcgaagg caagactgag
     1021 cgcgctcttc gggatccagt cctggtacac cgtcacatcc tcggtggtgt aggcgttgtt
     1081 gttgccgccg gccgcttcca gcaggcggtc gaaggtgccg ggcggccgcg tcttcgtgcc
     1141 gttgaacatc atgtgctcga agaaatgcga caggccggtg atgcccggcc gctcgttgcg
     1201 gctgcccacg cggaagaagg tgtagaccgc cacgctggga atgtcgtgcg acggccacac
     1261 cagcagctgc agcccgttgg ccagccggtg ttcgtgcacc gcgccggccg cgtgatcgtc
     1321 ggccgcctgc gccaccggcg ccgccagcaa caaggacgtt gcgaggattt tcttcagcat
     1381 gcgccgctcc tagaagtcgc gcagcatctc gcgcgccgcg ttgcgcccgc aggcgcccat
     1441 gacgccgccg cccggatgcg cggccgcgcc gcacaggtac agcccgcgca ccggcgtgcg
     1501 gtagtcgctc cagcccggca ccggccgcag cgagaacagc tggtgcagcg gcatggcgcc
     1561 ctggaagatg ttgccgccgg tgagcgcaaa gcgccgttcg aggtccagcg gcgacagcac
     1621 ctgctggtgc agcacgctgc gggcgaagtt gggagcatgg cgcgtgacct cggcgatgac
     1681 ctggtccgcg tacgactgcc cgatctcgtc ccacttccct tgcgccagcc ggtacggcgc
     1741 gtactgcacg aacaggttgg cgatgtgatg gcccggcggc gccagcgtgg catcgacgga
     1801 cgtcgggatg accatctcga tcaccgggcg gcggctgggc acgccgtact tggcgtcgtc
     1861 gtaggcgcgc tcgatctgct cgggcatggc gttgatgtgg atggtgccgc ggtgctgcgg
     1921 gcccagcgca gtgccggggc agcagcggaa atccggcagc tcggacagcg ccagattgat
     1981 cttcatgctg gcgctcgagt agtcgatgcg cgccaccgcc tcgcggaacg gccctggcag
     2041 gtgcctcgca tccagcagcg ctccgaaagt gacctgcgcc gtcgcgctcg aggccactgc
     2101 gaacgcgcgc agcacttctc cgccttgcag acgcacaccg accgcacgcc cgccttcgac
     2161 caggatttcc tctaccgcgg cgttgcagcg gatctccacg cccgcggcct gcgcgctgcg
     2221 cgccagcgcc tgggtgagcg cacccatccc gccttcgaca taaccccaca cgccacgcac
     2281 gccgccggcc atgcccatca cgtggtgcag cagcacgtag gcggtgccgg gggcggatgg
     2341 tggctggaac gtgccaatga tggcgtcggt cgccaacgtg cccttgagtt cgggcgattc
     2401 gaaccagcgg tcgaggatcg gcgtggcggc gccggtgaga atttccagtg cctgcggaat
     2461 gtcctcgccc aggtccgcaa gtgcacggta gagccggcgc gcgcgcagca ggtcgcgcag
     2521 gcgcgcgagc catccgcgcc ggcgccagct cgccggcagc ggcagcaatt ccggcgccgt
     2581 gtccatcagc acaggttcca ggtgctcggc gatgctcgtc agcagcgctt cgtagcgcgg
     2641 atacgcc
//
LOCUS       DASZZC010000062         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_645463,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000062 DASZZC010000000
VERSION     DASZZC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645463"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VHE37_01575"
     CDS             <1..1322
                     /locus_tag="VHE37_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT34246.1"
                     /translation="AALCGEAAAKVSPQEADRLGKELTPVGAEKAGNKDSSIPAWDGG
                     LNKSPACYKGAPNRYCDAFPDDMPLYTITKANLDQYKDKLSPGQLATFAIYGDTYKLN
                     VYPTRRTYANPDYVYEATKKNALNSELISNGEGIAGAILGVPFPIPKSGQEVVWNHKL
                     TYLGPGFTHWNEQAAVTASGEFNLVKLREDIKFPYGTQGMKPEDLNNVGIYFLQIVTE
                     PPRLAGTITLVHETMDQVKEPRRAWQYNPGQRRLRRAPNVGYDNPGTGSDGLRTNDQY
                     NMFNGATDRYTWKLVGKREMIVPYNSYVVHSDKYKVKDIVRKGHVNQDLLRYELHRVW
                     VVEAALKPGMSHLYARRLFYVDEDSWHMLLADNYDGRGQLWRWQESHAFQAYDRKVFV
                     TAMDVMYDLQSRRYIAMQMSNEDDEGVEKQFPAEYFDPANVQKQATK"
     gene            1521..1907
                     /locus_tag="VHE37_01580"
     CDS             1521..1907
                     /locus_tag="VHE37_01580"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34247.1"
                     /translation="MEAGKRSGGSRRKINGGVLGAAAEAVASPLVGNFKDFLGEAEAL
                     IRATAEFSSGTVAGARAQLQRKLDQAREMVNDVEEMAKQGYSKSVELSEKYVRENPWQ
                     IVAAAVALGFILGRMTGRGSPEESAG"
     gene            2034..2927
                     /gene="prpB"
                     /locus_tag="VHE37_01585"
     CDS             2034..2927
                     /gene="prpB"
                     /locus_tag="VHE37_01585"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HVT34248.1"
                     /translation="MPAPASPGRAFRDALAAEQPLQVPGTINANHALLARRAGFKAIY
                     LSGGGVAAGSLGLPDLGISGLEDVLIDVRRITDVCDLPLLVDVDTGFGSSAFNVARTT
                     KSLIKAGAAAMHIEDQVGAKRCGHRPNKEIVSKDEMVDRVKAAVDARTDEQFCIMART
                     DALAVEGLDRAIERAVACVQAGADMIFPEAMTSLDMYKKFSDAVKVPVLANITEFGST
                     PLFTVDELRDAGVGLVLYPLSAFRAMNKAAERVYGALRKDGTQKNVVDTMQTRMELYD
                     VIGYHDYEKKLDALFASAKKK"
     gene            3028..>3243
                     /locus_tag="VHE37_01590"
     CDS             3028..>3243
                     /locus_tag="VHE37_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HVT34249.1"
                     /translation="MSEATPAAGFKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGYD
                     ILDVAEQCEFEEIAHLIVHGKLPTRAEL"
BASE COUNT          643 a         1078 c         1068 g          454 t
ORIGIN      
        1 cagccgcgct gtgcggcgag gccgccgcca aggtcagccc gcaggaagcc gaccgcctgg
       61 gcaaggaact gactccggtt ggcgccgaaa aggccggcaa caaggacagc agcatcccgg
      121 cctgggatgg cgggctgaac aagtcgccgg cctgctacaa gggcgcgccg aaccgctatt
      181 gcgacgcctt ccccgacgac atgccgctgt acaccatcac caaggccaac ctcgaccagt
      241 acaaggacaa gctgtcgccg ggccagctgg cgacgttcgc catctacggc gacacctaca
      301 agctcaacgt ctacccgacg cggcgcacct acgccaaccc ggattacgta tacgaagcga
      361 ccaagaagaa tgcgctcaac agtgaactca tctccaacgg cgagggcatt gccggcgcca
      421 tcctgggcgt gccgttcccg atccccaagt cgggccagga agtggtgtgg aaccacaagc
      481 tcacttatct cggccccggc ttcacccact ggaacgagca ggcggcggtg accgccagcg
      541 gcgaattcaa cctggtgaag ctgcgcgagg acatcaagtt tccctacggc acgcagggca
      601 tgaagccgga agacctgaac aacgtcggca tctacttcct gcagatcgtc accgagccgc
      661 cgcggctggc cggcaccatc acgctggtgc acgagaccat ggaccaggtg aaggagccgc
      721 gccgcgcctg gcagtacaac ccgggccagc ggcgcctgcg ccgcgcgccc aacgtgggct
      781 acgacaaccc gggcaccggc tccgacggcc tgcgcaccaa cgaccagtac aacatgttca
      841 acggcgccac cgaccgctac acctggaagc tggtgggcaa gcgcgaaatg atcgtgccgt
      901 acaactccta cgtggtgcac agcgacaagt acaaggtgaa ggacatcgtg cgcaagggcc
      961 acgtcaacca ggacctgctg cgctacgagc tgcaccgcgt gtgggtggtg gaagccgcgc
     1021 tcaagccggg catgagccac ctgtacgcgc ggcggctgtt ctacgtggac gaggattcat
     1081 ggcacatgct gctggccgac aactacgacg gccgcggaca gctgtggcgc tggcaggaat
     1141 cgcacgcgtt ccaggcttac gaccgcaagg tgttcgtcac cgcgatggac gtgatgtacg
     1201 acctgcagag ccgccgctat atcgccatgc agatgagcaa cgaggacgac gagggcgtgg
     1261 agaagcagtt ccccgccgag tacttcgatc cggccaacgt gcagaagcag gccaccaagt
     1321 gacggccgcg tagcggccgt catccccgcg caggcgggga tccaggaaga gccggcggcg
     1381 cgccggcttt ttcttgcccg cgtagcagcc gtaatccccg cgccggctga tcttgcgcgc
     1441 gggaaaaata gcctggcgct gaacctgcgg cgcgcggcgc agtcctgcta taggtcaccc
     1501 gcagaccggc aggagcaacc atggaagcag gcaagcgcag cggcggttcg cgccgcaaga
     1561 tcaatggcgg cgtactcggc gcggcggcag aggcggtggc ctcgccgctg gtcggcaact
     1621 tcaaggactt tctcggcgag gccgaagccc tgatccgcgc gaccgcggag ttttccagcg
     1681 gcaccgtggc cggcgcccgc gcccagctgc agcgcaagct cgaccaggcg cgcgagatgg
     1741 tcaacgacgt cgaggagatg gccaagcagg gctacagcaa gtcggtcgaa ctgtcagaga
     1801 aatacgtgcg cgagaatccg tggcagatcg tcgccgcggc ggtggcgctg ggcttcatcc
     1861 tgggccgcat gaccgggcgc ggcagcccgg aggaatccgc cggctgaaat ccgcgcgggt
     1921 atttgcccct cccgaaacgg gcggggccgc ggggcgtgct acctgaatcc agcggcggcc
     1981 tttatcatgg cgcccccggc gcgccgccgc gcgccggcat ttcaggagtg acgatgcccg
     2041 cacccgcaag tcctggccgc gccttccgcg acgcgctcgc cgccgagcag ccgctgcagg
     2101 ttcctggcac gatcaacgcc aaccacgcct tgctggccag gcgcgccggc ttcaaggcga
     2161 tctacctgtc cggcggcggc gtggcggccg ggtcgctcgg cctgccggac ctgggcatct
     2221 ccggcctgga agacgtgctg atcgatgtgc gccgaatcac cgacgtgtgc gacctgccgc
     2281 tgctggtcga tgtcgatacc ggcttcggtt cttccgcctt caacgtggcg cgcaccacca
     2341 agagcctgat caaggccggc gccgcggcca tgcacatcga ggaccaggtg ggcgccaagc
     2401 gctgcggcca ccgcccgaac aaggaaatcg tcagcaagga cgagatggtc gatcgcgtga
     2461 aagcggcggt cgatgcacgc accgacgagc agttctgcat catggcgcgc accgacgcgc
     2521 tggcggtgga agggctagac cgcgccatcg agcgcgcggt ggcgtgcgtg caggccggcg
     2581 ccgacatgat cttccccgag gccatgacca gcctcgacat gtataagaaa ttctccgacg
     2641 cggtgaaggt gccggtgctc gccaacatca ccgaattcgg ctccacgccg ctgttcaccg
     2701 tggatgagct gcgcgatgcc ggcgtgggcc tggtgctgta cccgctgtcg gcgttccgcg
     2761 ccatgaacaa ggcggccgag cgcgtgtacg gcgcgctgcg caaggacggc acgcagaaga
     2821 acgtggtgga caccatgcag acgcgcatgg agctgtacga cgtgatcggt taccacgatt
     2881 acgagaagaa gctcgacgcg ctgttcgcca gcgcgaagaa gaaataatga cggccaaggg
     2941 caagaggtag gagcgcgcct tgcgcgcgat ccggtatcgc gggcaaggcc cgctcctaca
     3001 tgcggttatg accagaaggg aaatgaaatg agcgaagcaa ctccagcagc cggtttcaag
     3061 ccgaagaaat ccgtcgcgct gtccggcgtc accgccggca acacggcgct gtgcaccgtg
     3121 gggcgcaccg gcaacgacct gcactaccgc ggctacgaca tcctggatgt cgccgagcaa
     3181 tgcgagttcg aggaaatcgc ccacctcatc gtccacggca aactgccgac gcgtgctgaa
     3241 ctg
//
LOCUS       DASZZC010000063         6653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1226219,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000063 DASZZC010000000
VERSION     DASZZC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226219"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..537
                     /gene="tadA"
                     /locus_tag="VHE37_01595"
     CDS             82..537
                     /gene="tadA"
                     /locus_tag="VHE37_01595"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HVT34250.1"
                     /translation="MQPDDDERWMRHALALAQRARDAGEVPVGAVLVHGDELIGEGWN
                     QPIGAHDPSAHAEMVALRAGAQKLGNYRLTGSTLYVTLEPCVMCAGAIVHARVQRLVY
                     GAADPKAGAVQSVYDVIANPRLNHRVEWSGGVLEADCGDILREFFKARR"
     gene            complement(534..1199)
                     /locus_tag="VHE37_01600"
     CDS             complement(534..1199)
                     /locus_tag="VHE37_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVT34251.1"
                     /translation="MNEVRPLLLAIQNRDVAAARSALERDASQATDPVPGGLSPLMLA
                     LYHGARDIADLLRGFRAPDAYECAALNDARALAGLVSADVSLLEKHSADGWTLLHLAA
                     FFGARDAALVLIGLGAPLEARSTNPMENTPLHAALAGAAGESLAPLLVALGADVKTIG
                     GSGVGALHLAASRGFEPLCKLLLSRGAPLQAKTDDGKTAAALARERGHLATAAMLELA
                     AGG"
     gene            1311..1385
                     /locus_tag="VHE37_01605"
     tRNA            1311..1385
                     /locus_tag="VHE37_01605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1343..1345,aa:Val,seq:cac)
     gene            1525..2169
                     /locus_tag="VHE37_01610"
     CDS             1525..2169
                     /locus_tag="VHE37_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34252.1"
                     /translation="MGKTQTQAALQRFVPHRQGEGALLSADIKMHVGADGKSIGVFSI
                     DLKNAPTPDRKYVADLYDVVIAKADVRVLFAQSKVTGEGIRSLLVIHLSPAAIRRFLN
                     AVDDQNDGRFEKMAERLSVDVQPPTKIQEEPAGQTVAFSATAVMVAVQDYDACLDFMK
                     LSAFSLSNVLSTKQAAVEPIVRTDIHIGMLVGLLRALREAEKHFPKDRQEVGNG"
     gene            2592..3038
                     /locus_tag="VHE37_01615"
     CDS             2592..3038
                     /locus_tag="VHE37_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34253.1"
                     /translation="MVSRTPQQYLQRQTVKGGTAWYVRDNALLTDQRIHLLAWGHEAL
                     AVLVLSHGCEIDKKNSARVLVAPVQKIDALEEPVRTKVMSQQDWSLMPLPNVAGLGDC
                     FADLRLITPLQKPVLESCRKYSSMTDAAVARLKAQIHAFFFRSEPV"
     gene            3322..4041
                     /locus_tag="VHE37_01620"
     CDS             3322..4041
                     /locus_tag="VHE37_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34254.1"
                     /translation="MIASYLHNFIFVKTKKTAGTSAEIVMSPWVGPADVVTPMDPRDE
                     ILRADFGGKPQNFGRNRKAERNYMKVILGEDVAAIRAMRLSSPNRFREHMPARLIREE
                     LPDLWKVAYKFTIERHPYDKILSRAWMAIRRRQRDQAGELLSEKIDEVIERGLALNYP
                     MYMAKDKVLVDRIMPYEQMWKEVAALAQRLGKEMPLYLPQAKAQFRRDKRPASEILSA
                     AQKQRIYEKHAIEFELMGYQR"
     gene            4157..4480
                     /locus_tag="VHE37_01625"
     CDS             4157..4480
                     /locus_tag="VHE37_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34255.1"
                     /translation="MQTYITQVELDDDSQAAHDRLTAAMEAHGFSRVFLWRGDQRCLL
                     PPGHYCKAAASIEEVRSAARSAASLAAPWFSEFSCEMADREWSGVDFVSGLSWERAET
                     PAARA"
     gene            complement(4492..5730)
                     /locus_tag="VHE37_01630"
     CDS             complement(4492..5730)
                     /locus_tag="VHE37_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34256.1"
                     /translation="MKIARYLLLALACASAPAFADVFVSVRLAPPVMPVYEQPVVPGP
                     DYIWTPGYWAWDPEDGGYYWVPGAWVLAPQPGYLWTPGYWAFEYDVYRWHPGYWGPHI
                     GYYGGINYGFGYFGHGYEGGYWDGPHFRYNTAVTQVNVVNVHNTYHQTVINSVTNVTR
                     VSYNGGSGVHAQPTHDEQQAQHERHVGWTPQQNEHEQQARQMPEQHVSVNHGRPAVLA
                     TQRGTDRFADRSNRGDNGWSRGDHGNQGDTGWNRGNRGDTGTAGVQTPRNDNGARPWQ
                     GSTDRGDAAPDHGMPINRVQRTPQPVRHDNPQSWQQPERAPRVEAAEPAPQQQERRPQ
                     QGWQPQRDDGGWNERGQRQAPVQREMPEQRPVQQAPRGQYEQRGEGQRIERGPGPGQQ
                     NRGERPEREHPERDRQRNAY"
     gene            complement(5810..6472)
                     /locus_tag="VHE37_01635"
     CDS             complement(5810..6472)
                     /locus_tag="VHE37_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34257.1"
                     /translation="MKSPANLPAEDRQREPLPAGRHGLSRAQVSRSQRTRLLHAVVEA
                     VGEKGYAATTIADVAERADVSKKTFYVYFDGKDSCFLAAYEAFSDYLLKQMLEAGDGA
                     PTWRERYARGVRRYLELLAARPARTRALMLETLAAGPVVLKRRRAVLKTWTDHLRQLH
                     AQARSEDRRVADVPPRLFAITVGGIDELLREHIAGSGRIEHQAELAALILQNFDWLLR
                     VG"
BASE COUNT         1092 a         2117 c         2219 g         1225 t
ORIGIN      
        1 agtggatcaa gccgcagcac ctgcagatcg ccgccggcat cggcttcgtc gccgtcgggc
       61 tgtggacctt gcgcggagcc tatgcagcct gacgacgacg agcgctggat gcgccatgcg
      121 ctcgcgctgg cgcagcgggc gcgcgacgcc ggcgaggtgc cggtcggcgc ggtgctggtg
      181 cacggcgatg agctgatcgg cgaaggctgg aaccagccga tcggcgcgca cgatccttcc
      241 gcgcacgccg agatggtggc gctgcgtgcg ggcgcgcaga agctgggcaa ctatcgcctc
      301 accggcagca cgctctacgt cacgctggaa ccctgcgtga tgtgcgcagg cgccatcgtg
      361 catgcgcgcg tgcagcggct ggtgtacggc gcggccgatc cgaaggccgg ggcggtgcag
      421 tcggtctacg acgtcatcgc caacccgcgg ctgaatcacc gcgtggagtg gagcggcggc
      481 gtgctcgagg cggactgcgg cgacatcctg cgcgagttct tcaaggcgcg gcgttagccg
      541 ccggccgcca gctccagcat cgccgccgtc gccaggtgcc cgcgctcgcg cgccagcgcg
      601 gcggcggtct tgccgtcgtc ggtcttcgcc tgcagcggcg cgccgcgcga cagcagcagc
      661 ttgcacagcg gttcgaagcc gcgcgacgcc gcaaggtgca atgcgcctac tccgctgccg
      721 ccgatggtct tcacgtccgc gcccagcgcg accagcagcg gcgccagcga ctcgcccgcg
      781 gcgccggcca gcgccgcgtg cagcggcgtg ttctccatcg ggttggtgga gcgcgcttcc
      841 agcggcgcgc ccagcccgat cagcaccagc gccgcgtcgc gcgcgccgaa gaacgccgcc
      901 aggtgcagca gcgtccagcc gtcggcgctg tgcttttcca gcaacgaaac atctgcgctg
      961 accaggccgg ccagcgcacg cgcatcattg agcgccgcgc actcgtatgc atccggcgcg
     1021 cggaagccgc gcagcaggtc ggctatgtcg cgcgcgccgt ggtacagcgc cagcatcagc
     1081 ggcgacagcc cgccgggcac cgggtcggtg gcctggctgg cgtcgcgttc gagtgcgctc
     1141 ctggccgcgg ccacgtcgcg gttctggatg gccagcagca gcgggcggac ttcgttcatc
     1201 ggcgtatccc cagggcgagg gcgcgcagcc tagccgcgcg cgcgccggca ttcaacgccg
     1261 cgttgacgct gccgcaggtg ctcacctatc atgcgcgccc ccgatttcct gcacggttag
     1321 ctcagcggta gagcatcgct ttcacacggc gggggccaca ggttcgatcc ctgtaccgtg
     1381 caccaattca atgacttacg cgtgcttttt gagcgcgcgt atgatctctg actaaattct
     1441 gacgttggca atagtccccc cggccggggc ctttggtcgc gccggcagcc atctaaggtg
     1501 ctgattcatt cataattggt tcccatggga aagacccaaa cccaggcagc tctccagcgc
     1561 tttgtcccac ataggcaggg cgaaggggct cttttgtccg ccgacataaa aatgcatgtc
     1621 ggcgccgatg gaaagtccat cggggtgttt tcaattgacc tgaagaacgc gccgacgcct
     1681 gaccgaaaat acgttgcaga cctgtatgac gtcgtaattg caaaagctga tgttcgggtt
     1741 ctctttgcac agtcgaaggt caccggggag ggcattcgtt cgctcctggt catacatttg
     1801 agtccggcag caatcaggag atttttgaac gcggtagatg accaaaatga cggtcgtttt
     1861 gaaaaaatgg ccgaacggtt gtcggttgat gttcagcccc ctacaaaaat tcaagaagag
     1921 cctgcggggc agacggtcgc attctcagct actgcagtta tggtagcggt gcaggactac
     1981 gatgcttgct tggattttat gaagctatct gcgttctcac tctccaatgt gctgtcgacc
     2041 aaacaggctg ccgttgagcc gatcgttaga actgatattc atattgggat gcttgtagga
     2101 ttattgaggg cactccggga agctgaaaag cattttccga aggatcgcca ggaggtgggt
     2161 aatggttgaa tttaagaatt ttgacttggc cgcttggctc gtcgatggtt tgactcgtca
     2221 tgtaggcaag gtgcctgagc aagggcgcaa ggtcactacg cggatcggcc gtgtgatctc
     2281 cacggccgtg atgtctgccg cagttatttc agctgttggc ctatgcattg ttcctgtcga
     2341 ctctgtagct atgtcgactg gagtaatgac gtatcaagaa acgtcgacgg ttgcggcgcc
     2401 tgaatatgct gaagacattg tgccgccagg ctattgggcc aggctgatcg aaagatggct
     2461 gccgccactc ctctggccga aggcgacgta cagactccac catccgcgtt ctagtgtccg
     2521 tccaatcttg gtggacgaag acgcccagcg atttgctgag tcaaggcgac gtattggaca
     2581 gtgcgacatt catggtgtcg cgcacgccgc agcaatattt gcagcgccaa acggtgaagg
     2641 gcgggactgc ttggtacgtc cgcgacaatg cattgctcac agatcagcgc atccacctgc
     2701 tggcatgggg gcatgaagcg ctcgcggttc tagtgctgtc gcatggctgc gaaatcgaca
     2761 agaagaactc tgctcgtgtg cttgtcgctc cagtccaaaa aatagatgca ctggaagagc
     2821 cggtaagaac caaagtgatg tcgcaacaag attggtccct aatgccactg cctaacgtcg
     2881 ccgggttagg cgattgcttc gcagatctgc gattaatcac gccgcttcaa aagccggtgc
     2941 ttgaatcctg caggaaatat tcgtcaatga cagatgcggc tgtggcaaga cttaaggcgc
     3001 aaattcatgc tttcttcttt agatcggaac cagtctgagt ttcgttcgtc atccgatcta
     3061 agagggttat cgcgtcgcgg gcattctccg gcgccaaatg cgcatagcgc atcgtcgtcc
     3121 tgatgtcctt gtgccggcac agctcgcaca ccgtgcgcta ccgtgcacca aaacttccag
     3181 ccccgccttg agcggggctt tgttttgtcg cagcatgtgc aggccggttg cgccgccagg
     3241 ggtggcagcg caacgggatc ggatttagcg gttggggtcg gcctcgcggg aggccgtgcg
     3301 tcgttcgggc cggggaatgt cgtgatcgcg tcgtacctgc acaatttcat tttcgtcaag
     3361 accaagaaga ccgccggcac cagcgccgaa atcgtcatga gcccgtgggt cgggcccgcc
     3421 gacgtggtca cgccgatgga cccgcgcgac gaaatcctgc gcgcggattt cggcggcaag
     3481 ccgcagaact tcggccgcaa ccgcaaggcc gaacggaatt acatgaaggt gattctcggc
     3541 gaggacgtcg ccgccatccg cgccatgcgc ctgtcatcgc ccaaccgctt tcgcgaacac
     3601 atgccggcgc gcctgatccg cgaggaattg ccggacctgt ggaaggttgc ctacaagttc
     3661 accatcgagc gccacccgta cgacaagatc ctgtcgcggg cctggatggc catccggcgg
     3721 cggcagcgcg accaggcagg cgaactgctg tcggagaaga tcgacgaggt gatcgagcgt
     3781 ggactcgcgc tcaattaccc gatgtacatg gccaaggaca aggtgctggt cgaccgcatc
     3841 atgccctacg agcagatgtg gaaggaagtc gccgcgctgg cgcagcggct gggcaaggaa
     3901 atgccgctgt acctgccgca ggccaaggcc cagttcaggc gcgacaagcg cccggccagc
     3961 gaaatcctca gcgccgcgca gaagcaacgc atctacgaaa agcacgccat cgaattcgag
     4021 ctgatgggct accagcgcta ggcgcgctgt cgccgcgtgg gcggatgcac gcggccggtt
     4081 tcccgtctgc cgcaacggaa cttgcggccc gcatgcgccg cctaaccggt cgaaccctga
     4141 ccgcgggcgg ccggccgtgc agacctacat cacccaggtg gaactggacg acgacagcca
     4201 ggcggcgcat gaccggctga cagcggcgat ggaagcgcat ggcttctcgc gcgtgttcct
     4261 ctggcgcggc gaccagcgtt gcctgcttcc gcccggtcat tactgcaagg cagcggcctc
     4321 catcgaggaa gtgcgcagcg cggcgcgctc ggcggcctcg ctggccgcgc cctggttcag
     4381 cgagttcagc tgcgagatgg ccgaccgcga atggagcggc gtggatttcg tcagcgggct
     4441 cagctgggag cgcgcggaga ctcctgccgc gcgcgcctga gcaagcttca ttcaataggc
     4501 gttgcgctgg cgatcgcgtt ccggatgttc gcgttcgggg cgctcgcccc gattctgctg
     4561 gcccgggccc gggccgcgtt cgatccgctg gccttcgccg cgctgctcgt actgtccgcg
     4621 cggtgcctgc tgcaccgggc gctgctccgg catctcacgc tgcaccggcg cctgccgctg
     4681 cccacgctca ttccagccgc cgtcgtcgcg ctgcggctgc cagccctgct gcgggcgacg
     4741 ctcctgctgc tgcggtgccg gctcggccgc ctcgacgcgc ggagcgcgct ccggctgctg
     4801 ccaggactgc ggattgtcgt ggcgcaccgg ttgcggcgtg cgctggacgc ggttgatcgg
     4861 catgccgtga tccggcgccg cgtcgccgcg atcggtgctg ccctgccacg gccgcgcgcc
     4921 gttgtcgttg cgcggcgtct gcacgcctgc cgtgccggtg tctccgcggt tgccgcggtt
     4981 ccagccggtg tcgccctggt tgccgtggtc accccggctc cagccgttgt cgccgcggtt
     5041 gctgcggtcg gcgaagcggt cggtgccgcg ctgcgtcgcc agcaccgccg ggcggccgtg
     5101 gttcaccgac acgtgctgct ccggcatctg gcgcgcctgc tgctcgtgct cgttctgctg
     5161 cggcgtccag ccgacgtgcc gctcgtgctg cgcctgctgt tcgtcgtgcg tgggctgcgc
     5221 gtgcacgccg ctgccgccgt tgtagctgac gcgggtgaca ttggtgacac tgttgatcac
     5281 cgtctggtgg taggtgttgt gcacgttcac cacgttcacc tgggtgaccg cggtgttgta
     5341 gcggaagtgc gggccatccc agtagccgcc ttcatagcca tggccgaaat agccgaagcc
     5401 gtagttgatg ccgccgtagt agccgatatg cgggccccag tagcccgggt gccagcggta
     5461 cacgtcgtac tcgaaggccc agtagccggg cgtccacagg tatccgggct gcggcgccag
     5521 cacccaggcg ccgggcaccc agtagtagcc gccatcctcg ggatcccagg cccagtaacc
     5581 cggcgtccag atgtagtcgg gacccggcac caccggttgt tcgtagaccg gcatcaccgg
     5641 cggcgcgagg cgcaccgaca cgaacacgtc ggcgaaggcg ggggcgctgg cgcaggccag
     5701 ggcgagcagc aggtagcggg caattttcat ggcgacctct tggacgcggt catcggtttg
     5761 accggtgtgc ggccacccta gtgcgggcag gctgaacggc ggctgagcgt cagccgacgc
     5821 gcagcaacca gtcgaagttc tgcaggatca gcgcggccag ttcggcctgg tgttcgatgc
     5881 gtccgctgcc ggcgatatgt tcgcgcagca gttcgtcgat gccgcccacg gtgatggcga
     5941 acaggcgcgg cggcacgtcg gccacgcggc ggtcttcgct gcgcgcctgt gcatgcagct
     6001 ggcgcaggtg atcggtccag gtcttgagca cggcgcgtcg gcgcttgagc accaccggcc
     6061 cggcggcgag ggtttccagc atcagcgcgc gcgtgcgcgc cggccgcgcc gccagcagct
     6121 cgaggtaacg ccgcacgccg cgcgcatagc gttcgcgcca ggtcggggcg ccgtcgccgg
     6181 cctccagcat ctgcttgagc aggtaatcgg agaaggcttc gtaggcggcc aggaagcagc
     6241 tgtccttgcc gtcgaagtag acgtagaagg ttttcttcga cacgtcggcg cgctcggcga
     6301 cgtcggcgat ggtggtggcg gcgtaaccct tttctcccac cgcctcgacc accgcgtgca
     6361 gcaggcgcgt gcgctgcgac ctggacacct gtgcgcgcga caggccgtgg cgcccggctg
     6421 gcagcggttc gcgctgccgg tcttcagctg gcaagttggc ggggcttttc atctgcattg
     6481 cgacggtgga cggggccatg ctagcttaca gaaaccgatc ggtttccata accggaggca
     6541 acaggggccg cgcgagcctc gtggaggaaa cccgtgaacc cgatgatccg cccgttcctg
     6601 gtgttggtgt tgtgcgccct ggccgccggc tgcgtcagca gttggtccac gca
//
LOCUS       DASZZC010000064         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_581308,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000064 DASZZC010000000
VERSION     DASZZC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581308"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..883
                     /locus_tag="VHE37_01640"
     CDS             206..883
                     /locus_tag="VHE37_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HVT34258.1"
                     /translation="MNEIADRLHAVRQRVAAACAAAGRAPDSVRLLAVSKGHDAAAIE
                     QAGAAGQLEFGENYVSEALAKIAQLPREKHVWHFIGPLQSNKTREVAENFDWAHSVDR
                     LKTAQRLSAQRPAGAAPLNVCVQVNVSGEASKSGCAPAEALELCRAIAALPRLELRGL
                     MAIPAPGSGRAPYALLRSLRDEARRAGLALDTISAGMSDDLEDAIAEGSTLVRIGTAI
                     FGARNRQ"
     gene            880..1539
                     /gene="rpiA"
                     /locus_tag="VHE37_01645"
     CDS             880..1539
                     /gene="rpiA"
                     /locus_tag="VHE37_01645"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HVT34259.1"
                     /translation="MTQDEAKRAVAQAALKYIVPGEVLGVGTGSTVNHFIDALAAKKN
                     VIAGAVSSSNASTERLKKLGIEVLDLNNAGQLSVYVDGADEATEHLYLIKGGGAALTR
                     EKIVAQASRRFVCIADDSKLVPVLGRFPLPVEVIPMARSLVARELLKFGCQPELRTGV
                     TTDNGNVILDLHGMSITDPVTLEREINQIPGVVTVGLFARRPADVLLLGGPNGVREIK
                     R"
     gene            complement(1546..1818)
                     /locus_tag="VHE37_01650"
     CDS             complement(1546..1818)
                     /locus_tag="VHE37_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HVT34260.1"
                     /translation="MKRIFAGNLPDDSTEKEITELFSAHGKVRSIRLMQDVFSGKCKG
                     FGFIEMEGHEARAAIAALNGKDFRGKPLKVNEERPQEKRFGNQRRR"
     gene            complement(1853..>2537)
                     /locus_tag="VHE37_01655"
     CDS             complement(1853..>2537)
                     /locus_tag="VHE37_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HVT34261.1"
                     /translation="NRAADTVNQRVIHVHKGDKAGLLAYMISRGNWRQTLVFTRTKHG
                     ANRLAERLLRDGITTAALHGNKSQNARTKALAALKAGEVRVLVATDIAARGLDIDQLP
                     HVVNYELPNVPEDYVHRIGRTGRAGATGEAVSLVGPEERGELKDIERLLKKSIPTFEV
                     EGYVPGPRQAEEPRPPQQHRQGRRRGGGRPGGEGRGHGHGQRHGHGGRDHRGGQASKP
                     QHHSGGRHK"
BASE COUNT          382 a          849 c          875 g          431 t
ORIGIN      
        1 tggacctgct tgtggtccag cggcggaaga ttgatgcgct tcaggtcgcc gtcgacgcgg
       61 atcatcggct cgatgccggc cgacaggtgc aagtccgaag ccttctgctg cgtgctgaac
      121 gtgagcagct gcgcgatatc catggcgtct caaatcccct tgccggtctg tgccgacgtt
      181 agcccggttt atcctagcag gcaacatgaa cgagattgcg gatcgactgc acgccgtccg
      241 gcagcgcgtg gccgccgcct gtgccgctgc cggacgcgcg ccggacagcg tgcgcctgct
      301 ggcggtttcc aagggccacg acgcggcggc gatcgagcag gccggcgccg ccggccagct
      361 ggaattcggt gagaactacg tcagcgaagc gctggcgaag attgcgcagc tgccgcgcga
      421 aaagcatgtc tggcatttca tcggcccgct gcaatcgaac aagacgcgcg aggtggccga
      481 gaatttcgac tgggcgcaca gcgtcgaccg cctgaagacc gcgcagcggc tgtcggcgca
      541 gcggcccgcc ggcgccgcgc cgctgaacgt gtgcgtgcag gtcaatgttt cgggcgaagc
      601 gtccaagtcc ggctgcgccc cggcagaagc gctcgaactg tgccgcgcca tcgccgcgct
      661 gccgcgcctc gaactgcgcg ggctgatggc gattcccgca cccggcagcg ggcgcgcacc
      721 ttacgcgctg ttgcgctcgc tgcgcgatga ggcgcgacgc gccggcctcg cgctcgatac
      781 aatttccgcg ggcatgtcgg acgatctgga agacgcgatc gccgaaggct cgaccctggt
      841 gcgcatcggc accgccatct tcggggcaag aaacaggcaa tgactcagga cgaagccaaa
      901 cgcgccgtcg cacaggccgc gctcaagtac atcgtgccgg gcgaagtgct cggcgtgggc
      961 accggcagca cggtaaacca tttcatcgac gcgctggcgg cgaagaagaa tgtcatcgcc
     1021 ggcgcggtgt cgtcgtccaa tgcctcgacc gagcggctga agaagctcgg catcgaagtg
     1081 ctggacctga acaatgccgg ccagctgtcg gtgtatgtcg atggcgccga cgaggccacc
     1141 gagcacctct acctgatcaa gggcggcggc gcggcgctga cgcgcgagaa gatcgtggcg
     1201 caggccagcc gcaggttcgt gtgcatcgcc gacgacagca agctggtgcc ggtgctgggc
     1261 aggttcccgc tgccggtgga agtgatcccg atggcgcgct cgctggtggc gcgcgaactg
     1321 ctgaagttcg gctgccagcc ggagctgcgc accggcgtga ccaccgacaa cggcaacgtc
     1381 atcctcgacc tgcacggcat gagcatcacc gacccggtga cgctcgagcg cgagatcaac
     1441 cagatccccg gcgtggtcac cgtgggcctg ttcgcgcgcc gcccggccga cgtgctgctg
     1501 ctcggcgggc cgaacggcgt gcgcgaaatc aagcgctgaa gacgctcagc gccggcgctg
     1561 gttgccgaag cgcttttcct gcggccgctc ttcgttgacc ttgagcggct tgccgcggaa
     1621 gtccttgccg ttgagcgcgg cgatggcggc gcgcgcttcg tggccttcca tctcgatgaa
     1681 gccgaagccc ttgcacttgc cgctgaacac gtcctgcatc agccggatcg agcgcacctt
     1741 gccgtgcgcc gagaacagct cggtgatttc cttctcggtg ctgtcgtcgg gcaggttgcc
     1801 ggcaaaaatt cgcttcatgg tgcctcctgg aaactatgga tggggccgct tgttacttgt
     1861 gacggccgcc ggaatggtgc tgcggcttgc tggcctggcc gccgcggtga tcgcgcccgc
     1921 cgtggccgtg acgctggcca tgaccatgcc cgcggccttc accgcccgga cgtccgccgc
     1981 cgcgacggcg accctggcga tgctgttgcg gaggccgcgg ttcctcggcc tggcgcgggc
     2041 ccggcacgta accttcgacc tcgaaagtcg gaattgattt cttcagcagg cgctcgatgt
     2101 ccttgagctc gccgcgttcc tccggcccga ccagcgagac cgcctcgccg gtggcgccgg
     2161 cacggccggt gcggccgatg cggtgcacgt aatcctcggg cacattgggc agctcgtaat
     2221 tcaccacgtg cggcagctgg tcgatgtcca gcccgcgcgc ggcgatgtcg gtcgccacca
     2281 gcacgcgcac ctcgcccgcc ttgagcgcgg ccagcgcctt ggtgcgcgcg ttctggctct
     2341 tgttgccgtg cagggcggcg gtggtgatgc cgtcgcgcag caggcgttcc gccaggcggt
     2401 tggcgccatg cttggtgcgg gtgaacacta gcgtctggcg ccagttgccg cggctgatca
     2461 tgtaggccag cagcccggcc ttgtcgccct tgtgcacgtg gatcacccgc tggttcaccg
     2521 tgtcggccgc gcggttg
//
LOCUS       DASZZC010000065         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_325297,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000065 DASZZC010000000
VERSION     DASZZC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325297"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VHE37_01660"
     CDS             complement(<1..425)
                     /locus_tag="VHE37_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34262.1"
                     /translation="MSITLRRALTLFSCAACSALLLAPAHAGVPAAEAAKLGKELTDV
                     GAERAGNAAGTIPAYVGRESFSPEMLKITRKDLETLRVRLVQDIGQMLTDPAVTRDML
                     AQAQAVMDAEPAKADRVLQLVRAMLSSDAGLKAEFDRALS"
     gene            536..2023
                     /locus_tag="VHE37_01665"
     CDS             536..2023
                     /locus_tag="VHE37_01665"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT34263.1"
                     /translation="MSESEYEAIIVGAGFGGMGAAIQLQRLGYRRILMLDRADDLGGT
                     WHLNTYPGLAVDIASLSYSYSFEPNPYWSRLYAPGREIKAYADHVARKYGLRDCLRAG
                     TGVERAQWDERAQQWTVWPHGGAPVTARLLILATGFLSQPKKPDIPGVDSFAGKVIHT
                     AQWDHAYDFSGKRAAMIGTGASAVQVLPKIVPQVAHMDVYQRTPIWVLPKHNPRIAPW
                     LQKLFARVPLLQRLMRFWSNLILELVMVAGVLHNRQLPFLTRWVERGARRFLAHQVKD
                     PELRRKLTPDYSFGCKRPTFANDYFPMFTRPNVELVTERIERIEPDAIVTAGGKRREI
                     DTLILATGFNLWQKGNFPAFDVVGRGGVELGHWWNEHRYQSYEGITVPGFPNLFNIHS
                     PYAYSGFCYFNTIEVAMAHMDRCLREMQRRGAVTFEVTEQAKDRYMATMRARGADTVF
                     SAGNCASAHSYYFNQHGEATLLRLSSAWRALRSARNFPLDAYVYA"
     gene            complement(2197..2838)
                     /locus_tag="VHE37_01670"
     CDS             complement(2197..2838)
                     /locus_tag="VHE37_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34264.1"
                     /translation="MRETARGAALERPQRAQLDAATRARVEQAVLDIFSEREFHRVQL
                     IEVARAANVSLQTIYKYYGSKEALLFDSLDTWLSQLAARMLDHLQGIEDYKEKLRKVF
                     WVNIDFFEKNPKVAQIIMSSVYINTWRKQENYRNPMLFGAFLRVLGEGRARGILNSQV
                     DEKYLLDFIMGVMGRLVQMYIQRGQKQQLTQQANALFEMLWRGISKPAKNERG"
     gene            3030..>3758
                     /locus_tag="VHE37_01675"
     CDS             3030..>3758
                     /locus_tag="VHE37_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HVT34265.1"
                     /translation="MTDAFIYDAVRTPRGKGKKDGSLHQITPIHLLSNLFGALQQRNE
                     LDTSKVDDVVLGCVTPVGEQGADIARIAVLYAGWDQSVAGVTQSRFCASGLESVNLAA
                     MKVMSGQEDMVVAGGVESMSRWPMGSDGGAMAMDPQVNQRLGFAPQGIGADLIASLEG
                     FSRKDVDEFAVRSQQRAARAQAEGRFSKTLVPVKDVAGITVLDRDETVRGDTTVETLA
                     GLKPSFEQMGQMGFDATALRKYTTV"
BASE COUNT          633 a         1276 c         1201 g          648 t
ORIGIN      
        1 gacagcgcgc ggtcgaactc ggctttcagc ccggcatccg acgacagcat tgcgcgcacc
       61 agctgcagca cgcggtcggc cttggccggt tcggcatcca tcaccgcctg cgcctgcgcc
      121 agcatgtcgc gcgtgaccgc cgggtcggtg agcatctggc cgatgtcctg caccaggcgc
      181 acgcgcagcg tctccagatc cttgcgcgtg atcttgagca tctcgggcga gaatgactcg
      241 cgcccgacat acgccgggat ggtgccggcc gcgttgccgg cgcgctcggc gccgacgtcg
      301 gtcagctcct tgcccagctt cgcggcctcg gccgcgggca cgccggcatg ggccggtgcc
      361 agcaacagtg cggaacacgc cgcacacgaa aacagggtca gcgcccgccg cagggtaatg
      421 gacatcgccg cgctcctcgc atccgttcgt ggtggggcgg ctctctacaa ggcggccgct
      481 tgtcctgtag gttatggggc ggaccgtgcc taaagcaacc gatggcagca caaccatgag
      541 cgaatccgag tacgaagcga tcatcgtcgg cgccggcttc ggcggcatgg gcgcggccat
      601 ccagctgcag cgcctgggct accggcgcat cctgatgctc gatcgcgccg atgatctggg
      661 cggcacctgg cacctcaata cctatccggg cctggcggtc gacatcgcct cgctgtcgta
      721 ctcgtattcg ttcgagccga atccctactg gtcgcggctg tacgcgccgg gccgcgagat
      781 caaggcctat gccgatcatg tcgcccgcaa gtacggcctg cgcgattgcc tgcgcgcggg
      841 caccggcgtg gaacgcgcgc agtgggatga gcgcgcacag caatggacgg tgtggccgca
      901 cggcggcgcg ccagtcaccg cgcgcctgct gatcctggcc accggtttcc tgtcgcagcc
      961 gaagaagccg gatatccccg gggtcgattc cttcgccggc aaggtcatcc ataccgcgca
     1021 gtgggatcac gcctacgatt tttccggcaa gcgcgcagcg atgatcggca ccggcgccag
     1081 cgcggtgcag gtgctgccga agatcgtgcc gcaggtggcg cacatggacg tgtaccagcg
     1141 cacgccgatc tgggtgctgc ccaagcacaa cccgcgcatc gcgccgtggc tgcagaaact
     1201 cttcgcgcgc gtgcccctgc tgcagcgcct gatgcgcttc tggagcaacc tgatccttga
     1261 gctggtgatg gtggccggcg tgctgcacaa ccggcagttg ccgttcctca cccgctgggt
     1321 cgagcgcggc gcgcggcgtt tcctggcgca ccaggtcaag gacccggagc tgcggcgcaa
     1381 gctcacgccg gattattcat tcggctgcaa gcgtccgacg tttgccaacg attacttccc
     1441 gatgttcacg cgcccgaacg tggagctggt caccgagcgc atcgagcgca tcgagccgga
     1501 tgccatcgtc actgccggcg gcaagcgccg cgagatcgac acgctgatcc tggccaccgg
     1561 cttcaacctc tggcagaagg gcaacttccc ggcgttcgac gtggtcggcc gcggcggcgt
     1621 ggagctcggc cactggtgga acgagcaccg ctaccagtcg tacgagggca tcaccgtgcc
     1681 cggcttcccc aacctgttca acatccacag cccctacgcg tacagcggct tctgctactt
     1741 caacaccatc gaagtggcca tggcgcacat ggaccgctgc ctgcgcgaga tgcagcggcg
     1801 cggcgcggtg accttcgagg tgaccgagca ggccaaggac cgctacatgg cgacgatgcg
     1861 cgcgcgcggc gccgacaccg tgttcagcgc cggcaactgc gccagcgcgc acagctacta
     1921 cttcaaccag cacggcgaag cgacgctgct gcggctgtcg tcggcctggc gcgcgctgcg
     1981 ctcggcgcgg aattttccgc tcgacgctta cgtctacgcc tgaccctgcg cagcgggcgc
     2041 gcaaaaacac gtccaccgca ccggcatttg tgatgttata aataattgtc tattttttaa
     2101 tataaacaat aattaataac atcgattggc cgggtgcggt gacgtgcttt tttgagttgc
     2161 acgacggctc cgcgcttccg cagggacgac gaggaactag cctcgctcgt tcttcgccgg
     2221 cttcgagatg ccgcgccaga gcatttcgaa cagcgcgttg gcctgctgcg tcagctgctg
     2281 cttctgcccg cgctggatgt acatctgcac cagccgcccc atcacgccca tgatgaagtc
     2341 gagcaggtac ttctcgtcta cctggctgtt gaggatgccg cgcgcgcggc cttcgccgag
     2401 cacccgcagg aaggcgccga acagcatcgg gttgcggtag ttctcctgct tgcgccaggt
     2461 gttgatatac accgagctca tgatgatctg cgccaccttc gggttctttt caaaaaagtc
     2521 gatgttgacc cagaacacct tgcgcagctt ctccttgtag tcctcgatgc cctgcaggtg
     2581 gtcgagcatg cgcgccgcca gctgcgacag ccaggtgtcg aggctgtcga acagcagcgc
     2641 ttccttgctg ccgtagtact tgtagatggt ctgcaggctg acgttggctg cgcgcgccac
     2701 ctcgatcagc tgcacgcggt ggaactcgcg ctcggagaaa atatccagca ccgcctgctc
     2761 cacgcgcgca cgcgtggcgg cgtccagctg tgcgcgctgc ggccgctcca gcgcggcgcc
     2821 gcgtgccgtc tcccgcatcc tggtaagacc tgttaccgcc atggctgtcc tctcaatacg
     2881 ccgacgtatt gaccggccgg cgcgctcttg cttatgctgc gccgccgcat caatccgggc
     2941 ggctgctgga aaccccgcca tcatcgcggg cgcgctgatg gtaatttgga ttaccccgac
     3001 acgcaacgat tcctcaacga gaaatctcca tgaccgacgc attcatctac gatgccgtgc
     3061 gcaccccgcg cggcaagggc aagaaggacg gcagcctgca ccagatcacg cccatccacc
     3121 tgctgtcgaa cctgttcggc gcgctgcagc agcgcaacga gctcgacacc tccaaggtcg
     3181 acgacgtggt gctcggctgc gtcacgccgg taggtgaaca gggcgcggac atcgcgcgca
     3241 tcgccgtgct ctacgccggc tgggaccaga gcgtggccgg cgtcacccag agccgcttct
     3301 gcgcctcggg tcttgaatcg gtgaacctgg ccgcgatgaa ggtgatgagc ggccaggaag
     3361 acatggtggt cgccggcggc gtggagagca tgagccgctg gccgatgggc agcgacggcg
     3421 gcgccatggc catggacccg caggtcaacc agcgcctggg cttcgccccg cagggcatcg
     3481 gcgccgacct catcgcctcg ctggaaggtt tctcgcgcaa ggatgtcgac gagttcgccg
     3541 tgcgctcgca gcagcgcgct gcgcgcgcgc aggcggaagg ccgcttcagc aagacactgg
     3601 tcccggtgaa ggacgtggcc ggcatcaccg tgctggaccg cgacgaaacc gtgcgcggcg
     3661 acaccacggt ggaaacgctc gcgggcctca agccctcgtt cgagcagatg ggccagatgg
     3721 gcttcgacgc caccgccctg cgcaagtaca cgacggtg
//
LOCUS       DASZZC010000066         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_904803,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000066 DASZZC010000000
VERSION     DASZZC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904803"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1055
                     /gene="fliM"
                     /locus_tag="VHE37_01680"
     CDS             75..1055
                     /gene="fliM"
                     /locus_tag="VHE37_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HVT34266.1"
                     /translation="MSSKDLLSQDEIDALLHGVDSGKVDTEPAPLAPGEARKFDFTSQ
                     DRIVRGRMPTLEMINERFARLFRIGLFNMLRRSPELSVAGIEMMKFGEYTHSLFVPTS
                     LNLIRVKPLRGTALMIFEPRLVFSVVENFFGGDGRIATKIEGREFTPTEMRVIQLLVK
                     QAFTDLTEAWQPVMPLEYEYLNSEVNPHFANIVSPSEIVVVSKFKIELEGGGGHLHVT
                     FPYSMLEPIRGELDAGVQSDRMERDERWAFALRDQIKEAPVAITSELASAHITLRELL
                     TLKPGDVVPINMPKTVDVCVEELPVFRGSFGVSNGRNSVRITEVINRTKH"
     gene            1062..1400
                     /gene="fliN"
                     /locus_tag="VHE37_01685"
     CDS             1062..1400
                     /gene="fliN"
                     /locus_tag="VHE37_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HVT34267.1"
                     /translation="MEPTANPAPAAPAKPKLDAASLAATGDLNLEVILDVPVTLSMEV
                     GRARIPIRNLLQLNQGSVVELERAAGEPLDVYANGTLIAHGEVVVVNEKFGVRLTDVV
                     SPVERIRKLK"
     gene            1462..1782
                     /locus_tag="VHE37_01690"
     CDS             1462..1782
                     /locus_tag="VHE37_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HVT34268.1"
                     /translation="MSTAASLLQMLLSLGLVLGAIFGLAWLVRRTQSLTPRAAGDVAV
                     LAAAQVGAKERVLLLNAGGTHVLIGVAPGSVRTLHVYAEPPQLAERPSPPAFAEALKK
                     ALGR"
     gene            1779..2522
                     /gene="fliP"
                     /locus_tag="VHE37_01695"
     CDS             1779..2522
                     /gene="fliP"
                     /locus_tag="VHE37_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650226.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HVT34269.1"
                     /translation="MRSFVRLAALLLLAAAPGAFAAGLPAVTMTPAPGGGQSYALSLQ
                     VLLLMTALTLLPAALLMMTAFTRIVIVLGLLRHALGTGQTPSNQILVGLSLFLTLFVM
                     QPVFERVYNEAAQPYLDGKMEFQPALAAAEKPVRAFMLAQTRQADLLTFAKIAGPGKY
                     ATPDDVPFTVLAASFMTSELTTAFQIGFLLFIPFVVIDLVVSSVLMSMGMMMLSPMLI
                     SLPFKLMLFVLVDGWSLVMGSIAASFHPV"
     gene            2519..>2659
                     /locus_tag="VHE37_01700"
     CDS             2519..>2659
                     /locus_tag="VHE37_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HVT34270.1"
                     /translation="MSPETVLTTGRDALQLALLLSAPLLLASLVVGVLVGVFQAATQI
                     NEM"
BASE COUNT          414 a          906 c          864 g          475 t
ORIGIN      
        1 cttcgctcct ctcgcctagc ctggcgcgtc ttggtgcagc aacgcttgcg cgaagtgagc
       61 ctaggcaggc cctcatgagc tcgaaggatc tgctgtcgca ggacgaaatc gacgcgctgc
      121 tgcacggcgt cgacagcggc aaggtcgaca ccgaacccgc gccgctggcg ccgggcgagg
      181 cgcgcaagtt cgatttcacc tcgcaggacc gcatcgtccg cgggcgcatg ccgacgctgg
      241 agatgatcaa cgagcgcttc gcccgcctgt tccgcatcgg cctgttcaac atgctgcgcc
      301 gctcgccgga gctgtcggtg gccggcatcg agatgatgaa gttcggcgag tacacgcact
      361 cgctgttcgt gcccacctcg ctcaatctga tccgggtgaa gccgctgcgc ggcaccgcgc
      421 tgatgatctt cgagccgcgc ctggtgttct cggtggtgga gaatttcttc ggcggcgacg
      481 gccgcatcgc caccaagatc gagggccgcg agttcacgcc caccgagatg cgcgtgatcc
      541 agctgctggt caagcaggcc ttcaccgacc tgaccgaggc ctggcagccg gtgatgccgc
      601 tggaatacga atacctgaac tccgaggtca acccgcactt cgccaacatc gtctcgccgt
      661 cggagatcgt ggtggtgtcg aagttcaaga tcgagctgga aggcggcggc ggccacctgc
      721 atgtgacctt tccctactcg atgctcgaac cgatccgcgg cgaactcgac gccggcgtgc
      781 agtccgaccg catggagcgc gacgagcgct gggcctttgc gttgcgcgac cagatcaagg
      841 aggcgccggt ggccatcacc tccgagctcg cctccgccca catcacgctg cgcgagctgc
      901 tcacgctcaa gcccggcgac gtggtgccca tcaacatgcc caagaccgtc gacgtctgcg
      961 tggaggaact gccggttttc cgcggcagct tcggcgtgtc gaacgggcgc aattccgtgc
     1021 gcatcaccga agtcatcaac cgaaccaagc actgagccat catggaaccg actgccaacc
     1081 ccgcgcccgc tgcgcccgcc aaacccaagc tcgatgccgc cagcctggcg gcgacgggcg
     1141 atctcaacct cgaggtgatc ctcgacgtgc cggtcacgct gtcgatggaa gtcggccgcg
     1201 cgcgcattcc catccgcaac ctgctgcagc tgaaccaggg cagcgtggtg gaactggagc
     1261 gcgccgccgg cgagccgctc gacgtctacg ccaacggcac gctcatcgcc catggcgaag
     1321 tggtcgtggt caacgagaaa ttcggcgtgc gcctgaccga cgtggtcagc ccggtcgagc
     1381 gcatccgcaa gttgaaatga gttgccatcg gcaacccatt tcaatccccg cgaaagcggg
     1441 gacccatgcc ggaatcgaat gatgtccact gctgcctcac ttctgcagat gctgctgtcg
     1501 ctcggcttgg tgctgggcgc catctttggc ctggcctggc tggtgcgccg cacgcagtcg
     1561 ctcacgccgc gcgccgccgg cgatgtggcg gtgctggcgg ccgcgcaggt cggcgccaag
     1621 gagcgcgtgc tgctgctcaa tgctggcggc acgcatgtgt tgatcggcgt ggcgccgggc
     1681 tcggtgcgca cgctgcatgt ctatgcagag ccgccgcaat tggcggagcg gccgtcaccg
     1741 ccggcgttcg ccgaggcgct gaagaaagcg ctgggccgat gagatcgttc gtcagactgg
     1801 ccgcattgct gttgctggcc gccgctcccg gcgccttcgc cgccggcctg cccgcggtga
     1861 ccatgacgcc cgctcctggc ggcggacaga gctacgcgct gtcgctgcag gtgctgctgc
     1921 tgatgacggc gctcacgctg ctgccggcgg cgttgctgat gatgaccgcc ttcacccgca
     1981 tcgtcatcgt gctcggcctg ctgcgccatg cgctgggcac cgggcagacg ccgtcgaacc
     2041 agatcctggt gggcctttcg ctgttcctca cgctgttcgt gatgcagccg gtgttcgagc
     2101 gcgtatacaa cgaggcggcg cagccgtacc tcgacggcaa gatggaattc cagccggcgc
     2161 tggccgcggc ggaaaagccg gtgcgcgcct tcatgctggc gcagacccgc caggccgacc
     2221 tgctcacctt cgccaagatc gccggccccg gcaagtacgc cacgcccgac gacgtgccct
     2281 tcacggtgct cgctgcatcg ttcatgacca gcgagctgac caccgcattc cagatcggct
     2341 tcctgctgtt cattccgttc gtggtcatcg acctggtcgt gtccagcgtg ctgatgagca
     2401 tgggcatgat gatgctgtcg cccatgctga tctcgctgcc gttcaagctg atgctgttcg
     2461 tgctggtcga cggctggagc ctggtgatgg gctcgatcgc cgcgtcgttc cacccggtat
     2521 gagcccggaa accgtactga ccaccgggcg cgacgcgctg cagctggcgc tgctgctgtc
     2581 ggcgccgctg ctgctggcct cgctggtggt cggcgtgctg gtcggcgtgt tccaggcggc
     2641 cacgcagatc aacgagatg
//
LOCUS       DASZZC010000067         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1097971,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000067 DASZZC010000000
VERSION     DASZZC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097971"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VHE37_01705"
     CDS             complement(<1..789)
                     /locus_tag="VHE37_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT34271.1"
                     /translation="MLSVIPPMTQLNTPYPSTAYLTGFLRSRGIDAVQEDLALALILR
                     LFSAAGLAAIRDAALAPPARRRTPCVRAFLQHYPRYAATVDAAIAFLQGRDPTLAHRI
                     AGRSFLPEGARFAPLEQYTDDGGGDALAWAFGALGLHDRARHLATLYLNDLADVLRDA
                     VDPRFEFVRYAESLAQSAASFDPLADALAAPHHLVDTTLAQLTLAALARHQPRVVLLS
                     VPFPGSVYAAFRIAQSVKAHDASIVTVLGGGYVNTELRALAEPRV"
     gene            930..1403
                     /locus_tag="VHE37_01710"
     CDS             930..1403
                     /locus_tag="VHE37_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34272.1"
                     /translation="MQQDSEPAVEVQQRKREYSLLLIEDNPGDVVMMREALRENGLPV
                     ELQVSRHGEGALHQLQHAAAHGEALPDLILLDLNLPSMHGLEVLAAIKSDPALQQIPV
                     LILTTSTAARDVENSYRLHANSYLTKPMDFSRFVALVRAVHDFWLSLSVPPATTH"
     gene            1562..>3347
                     /locus_tag="VHE37_01715"
     CDS             1562..>3347
                     /locus_tag="VHE37_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT34273.1"
                     /translation="MDFIKAEETRRQEKQPAPHPRTLGWLGTTALAMGGSNQSLFIIG
                     ALFIGQGDILGQGSAAVPLLIAGLLLGWAAAPGWTELVLMYPNRVGGISASCVEAFRP
                     YSAVLANLTGVCYWWGWVPTCGLTAILSASAIHHWYLPQVPIDLMAVGLVLFFMGVNL
                     AGIRWVSRLAIPIASTSALLAFISGLAPIVSGQVDWHQATTFTLTTPFAGWFGGLTSL
                     MAGLYLVGFAAPAFEAAACHVGETANPNRNVPRAMLASGLLAGLYFVVLPVVWLGALG
                     EKPLGEDLALVLGPTYAPLFGNAAKAAAIWFMMFSMFSGTLQPLAGAARTLAQLSEDG
                     LLPRFLALRSRTDAPWAATLLTAAMAILFLLIGDPIWLVAAANFTYLIGIALPSVAVW
                     LLRKDAPELPRPYRAPRGTLALGLTAAIVWGISALLGFEQFGLPTVVIGLAFAYSGSA
                     LYAWRRWSDRRAAGLPGMVYGLQFKLTGAMLLVLALDGVGYLLAVSNLSQQHSALLTG
                     LSDIFVAVAMLTVAVALVLPGMIAHSAMQVSEAAQRLAGGTIAEFSRAMRALGRGDID
                     AAHARVDVMPVRVYARDEVGEMAQSFNTL"
BASE COUNT          477 a         1147 c         1185 g          538 t
ORIGIN      
        1 cacgcgcggt tcggccagcg cgcgcagctc ggtgttcacg tagccgccgc cgagcacggt
       61 gacgatggac gcgtcgtgcg ccttcaccga ctgcgcaatg cggaacgcgg cgtacaccga
      121 gccgggaaac ggcaccgaca gcagcaccac gcgcggctgg tgccgcgcca gcgccgcgag
      181 cgtcagctgc gccagcgtgg tgtcgaccag gtgatgcggc gcggcgagcg cgtcagccag
      241 cgggtcgaac gacgccgcgc tctgcgccag cgactcggca tagcgcacga actcgaagcg
      301 cgggtcgact gcgtcgcgca gcacgtcggc caggtcattc aaatagagtg tcgccaggtg
      361 gcgcgcgcgg tcatgcaggc ccagcgcgcc gaaggcccag gccagcgcgt cgccgccgcc
      421 gtcgtcggtg tattgctcca gcggcgcaaa gcgcgcgcct tccggcagga aactccggcc
      481 ggcgatgcgg tgcgccagcg tggggtcgcg gccttgcagg aaggcgatgg ccgcatccac
      541 ggtggcggcg tagcgcgggt aatgctgcag gaacgcgcgc acgcacggcg tgcgccggcg
      601 cgcgggcggc gccagcgcgg cgtcgcggat ggcggccagg cctgccgcgc tgaacaggcg
      661 caggatcagc gccagtgcca ggtcttcctg caccgcgtcg atcccgcgcg agcgcaggaa
      721 gccggtgagg taggcggtcg atggatacgg cgtgttcagt tgcgtcatcg gcgggatgac
      781 ggacagcacg cggggcgcgg gtgcggacat ggcggcggat catagcaacg cacatcgctc
      841 aagaatttcc gcgctgcgcc gatagccatt acggaatcgc agccgttcga ctcgggtggg
      901 tccggcaatg ccgatcatgg aaacgacgca tgcagcaaga cagcgaaccg gccgttgaag
      961 tgcagcaacg caagcgcgag tactcgctgc tgctgatcga ggacaatccc ggcgacgtgg
     1021 tgatgatgcg cgaggcgctg cgcgagaacg gcctgccggt ggagctgcag gtgtcgcgcc
     1081 acggcgaggg cgcgctgcac cagctgcagc atgccgccgc ccatggcgaa gcgctgccgg
     1141 acctgatcct gctcgatctc aacctgccca gcatgcacgg gctggaagtg ctggcggcga
     1201 tcaagtcaga tccggcgctg cagcagatcc cggtactgat cctcaccacc tccaccgcgg
     1261 cgcgcgacgt ggagaattcc tatcgcctgc acgccaacag ctacctgacc aagccgatgg
     1321 atttctcgcg cttcgttgcg ctggtgcgcg cggtgcacga cttctggctc agcctgtcgg
     1381 tgccgccggc gacgacgcac tgacgtcatc gtcagtgcct gtgccggaac ggcaccgctc
     1441 gatgcgcggc atgccaccgc gcatctgctc ggtaggaatt gtctagctgc gaggggcctg
     1501 cactggataa ctaatccgta aggtggcgcc gatcgccatg atgggtggcg tagggacacg
     1561 catggacttc atcaaagcag aagaaacgcg tcgccaggaa aagcagccgg cgccgcatcc
     1621 gcgcaccttg ggctggctcg gtaccaccgc gctggccatg ggcggcagca accagagcct
     1681 gttcatcatc ggcgcgctgt tcatcggcca gggcgacatc ctgggccagg gcagcgcggc
     1741 ggtgccgctg ctcatcgccg ggctgctgct cggctgggcg gccgcgcccg gctggaccga
     1801 gctggtgctg atgtacccca accgcgtcgg cggaatttcc gcgagctgcg tcgaagcgtt
     1861 tcgcccgtac agcgccgtgc tcgccaacct caccggcgtc tgctactggt ggggctgggt
     1921 gccgacctgc gggctgaccg cgatcctgtc cgcatctgcc atccaccact ggtacctgcc
     1981 gcaggtgccg atcgacctga tggcggtggg cctggtgctg ttcttcatgg gcgtcaacct
     2041 ggccggcatc cgctgggtga gccgcctggc gattcccatt gccagcactt cggcactgct
     2101 cgccttcatc tccgggctgg cgcccatcgt ctccggccag gtcgactggc accaggcgac
     2161 cacgttcacg ctgaccacgc cgttcgccgg ctggttcggc ggcctgacca gcctgatggc
     2221 cggcctgtac ctggtgggct tcgccgcgcc ggccttcgag gcggcggcct gccacgtggg
     2281 cgagaccgcc aaccccaacc gcaacgtgcc gcgcgccatg ctggccagcg gcctgctcgc
     2341 cggcctgtac ttcgtggtgc tgccggtggt gtggctcggt gcgctcggcg agaagccgct
     2401 gggcgaagac ctcgcgctgg tgctggggcc aacctacgcg ccgctgttcg gcaacgcggc
     2461 caaggctgcg gcgatctggt tcatgatgtt ctcgatgttc tcgggcacgc tgcagccgct
     2521 ggccggcgcg gcgcgcacgc tggcgcagct gtcggaggac gggctgctgc cgcgtttcct
     2581 ggcgctgcgt tcgcgcaccg atgcgccgtg ggcggcgact ctgctcaccg cggcgatggc
     2641 catcctgttc ctgctgatcg gcgatccgat ctggctggtg gcggcggcca acttcactta
     2701 cctgatcggc atcgccctgc cgagtgtcgc ggtgtggctg ctgcgcaagg atgcgccgga
     2761 actgccgcgg ccctaccgcg cgccgcgcgg caccctggcg ctggggctca ccgccgccat
     2821 cgtctggggc atctcggccc tgctcggctt cgaacagttc ggcctgccga cggtggtcat
     2881 aggcctggcc ttcgcatact ccgggtccgc gctctacgcg tggcggcgct ggtccgaccg
     2941 ccgcgcagcc ggcttgccgg gcatggtcta cggcctgcag ttcaagctca ccggcgccat
     3001 gctgctggtg ctggcgctcg atggtgtcgg ctacctgctc gccgtgagca acctgtcgca
     3061 gcagcacagc gcgctgctca ccgggctgtc cgacatcttc gtcgccgtgg ccatgctcac
     3121 cgtggcggta gcgctggtgc tgccgggcat gatcgcgcac tcggcgatgc aggtttccga
     3181 ggcggcgcag cgcctggccg gcggcaccat cgccgagttc tcgcgcgcca tgcgcgcttt
     3241 gggccggggc gacatcgacg cggcgcatgc gcgcgttgac gtcatgccgg tgcgcgtcta
     3301 cgcgcgcgac gaggtgggcg agatggcgca gagcttcaac acgctgc
//
LOCUS       DASZZC010000068         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_773935,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000068 DASZZC010000000
VERSION     DASZZC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773935"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VHE37_01720"
     CDS             complement(<1..1703)
                     /locus_tag="VHE37_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34274.1"
                     /translation="MLFIVPAQRGAGIGNARRPLADALAGPAACWIPVMHLCRPRMLL
                     LAALAPALVHAQAAVVSGIDPSASVSAALALALREPAADRGNFSLYVGAQASTLALAK
                     VVIWIDGEEAAHYSYSEDQARSLAAGALQPLYRHELAAGAHRLRALAIAVDPQAPPYS
                     PRSRVQLEAALPDAGAQTVQVELRFTQGSAISTSALTLQPLPDAASAAQRAADFLLDD
                     QRPLEAAEQLAGTDADDASPRLMRIRQQLGLDAPVAIAAQPGLDEYDQAVAQLGAGDS
                     ARALDLLDALGRRSDIAAPLADRANLALGDALLRAHRNAPAGAAYARVHRDSAWASAA
                     LLGLGWSHLLSPNAATEAQPASYALPADADQAAQARRAVPFRYYNSVVSGARADDVRQ
                     ALLAWTELIGRDPADPSVQEGMLAVPYAMSHLGAQAQAEDYALRAITRLAAVHALLRD
                     GAALLHEQLPMLLQHYFSERSDGWHSQFADLTAERRWWLEGSGDERIRDVLAVLMQEP
                     AFRVALRDERDALLLRDFCARRAAATLPAADAQLQARQARLLEIGEQLIARRAHDTAN
                     NL"
     gene            1819..2301
                     /locus_tag="VHE37_01725"
     CDS             1819..2301
                     /locus_tag="VHE37_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34275.1"
                     /translation="MKILLPLCLSLLLCACAWVTPYLDGTYNAAAFPPLDAPADSVAL
                     VGRAADDARGAAVLTLVDDIPIKVKPDGSGDGLWAKQVALAPGEHALVTSWRRPGEYS
                     PSSSLCQAEFLAGHQYLVNYEVAASGREARVWLSDAADAGSTVNCAARKPPPMPQWPF
                     "
     gene            2343..2810
                     /locus_tag="VHE37_01730"
     CDS             2343..2810
                     /locus_tag="VHE37_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34276.1"
                     /translation="MNEIQQMVYSLQRFHILALYANTSTERTVSASYAYAWWQGIYPL
                     LNDSAPWHKPHTQSFSAPAQQVAELHEFLTQRWRAQDPISFFQLEDRYAIRAANRPGP
                     VWSQAQLIAACRYLHLHAQYDEEFWSALLGASQSPLPAEIITRPVEPAEIQFE"
     gene            complement(2881..3426)
                     /locus_tag="VHE37_01735"
     CDS             complement(2881..3426)
                     /locus_tag="VHE37_01735"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT34277.1"
                     /translation="MAWPASRSRPATCWPRACASPRSASSSPASPGWLQKAGLIELDA
                     QIGRHVPELPPEAGEPTRRQLMTHMGGTRCHLDLSLVSNALSPIPRGAPLAYHTRQRA
                     ADFAPGAQMIYCNGGYQLLSFAMERCAGTTLERIHAERIFEPLAMRDSGLVRSDLDCV
                     PGLADLHVPRGPSTAPYICLL"
     gene            complement(3538..>4075)
                     /locus_tag="VHE37_01740"
     CDS             complement(3538..>4075)
                     /locus_tag="VHE37_01740"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HVT34278.1"
                     /translation="KWTGPVIAALLSAVAVNLLAANLAPRAISWRVMRGIAQAAGGDN
                     RAVHAPRADASARAIVRPSPDLLYSACVFNLRDRALRVQAPVPDSYFSIAGFAANTDN
                     FFALNDSQLQADASGQKHFDLVITRAGLPVQAPAGARVIEAPGERGVILFRALIADDG
                     KLAELRDRFQAQQDCAPL"
BASE COUNT          634 a         1373 c         1465 g          603 t
ORIGIN      
        1 aggttgttcg ccgtgtcgtg cgcgcgccgg gcgatcagct gctcgccgat ttcgagcagg
       61 cgcgcctgcc gtgcctgcag ctgggcgtcg gcagcaggca gtgtggcggc cgcgcgtcgc
      121 gcacagaaat cgcgcagcag cagcgcgtcg cgctcgtcgc gcagggccac gcggaacgcc
      181 ggctcctgca tcagtaccgc cagcacgtcc ctgatgcgct cgtcgccgct gccctccagc
      241 caccagcgcc gctccgccgt caggtcggcg aactggctgt gccagccgtc gctgcgctcg
      301 gaaaaatagt gctgcagcag catgggcagc tgctcatgca gcagcgcggc accgtcgcgc
      361 agcagcgcat gcaccgcggc caggcgcgtg atagcgcgca gcgcatagtc ctcggcctgc
      421 gcctgtgcgc cgagatggct catggcgtag ggcactgcga gcatgccttc ctgtaccgac
      481 ggatcggcgg gatcgcgccc aatcagctcg gtccacgcca gcagcgcctg cctgacatcg
      541 tcggcgcgcg caccggagac cacgctgttg tagtagcgga acggcaccgc gcggcgcgcc
      601 tgcgcggcct ggtccgcgtc ggcgggcaat gcataggaag ccggctgcgc ttcggtcgcg
      661 gcattgggcg acagcaggtg cgaccagccg agcccgagca gcgcggcact ggcccatgcg
      721 ctgtcgcggt gcacgcgagc gtaggcggca cctgccggcg cgttacggtg cgcgcgcagc
      781 agcgcgtcgc cgagcgcgag attggcacgg tccgccagcg gcgcggcgat gtcgctgcgc
      841 cggcccagtg cgtccagcag gtcgagtgcg cgcgcggaat cgccggcgcc gagttgcgcg
      901 acggcctggt catattcatc gagcccgggc tgcgcagcga tcgccaccgg tgcgtcgagc
      961 ccgagctgct ggcggatgcg catcaagcgc ggcgacgcgt cgtccgcgtc ggtgccggcc
     1021 agctgctcgg ctgcttccag cggacgctgg tcatcgagca ggaaatcggc cgcacgctgc
     1081 gccgccgacg ctgcgtcagg cagcggttgc agcgtcagcg cagaggtcga gatcgccgag
     1141 ccctgcgtga aacgcaattc gacctgcacg gtctgcgcgc cagcgtcggg caaggcggct
     1201 tcgagctgca cgcggctgcg cggactgtac ggcggcgcct gcggatcgac ggcaatcgcc
     1261 agcgcccgca ggcggtgcgc gccggccgcc agttcatgcc ggtacagcgg ctgcagcgca
     1321 ccggcggcga gcgagcgggc ctggtcctcg ctgtaggaat agtgcgcggc ttcttcgccg
     1381 tcgatccaga tgacgacctt ggccagcgcg agcgtgctgg cctgcgcccc cacatataag
     1441 gagaagttgc cgcgatccgc tgccggctcg cgcaatgcaa gcgccagcgc ggcggaaacg
     1501 cttgccgatg gatcgatgcc ggaaacgacg gctgcttgcg catgcaccaa cgcgggcgcg
     1561 agcgcggcca ggagcagcat gcgggggcgg cacaggtgca tcacgggtat ccagcaggca
     1621 gccgggccgg caagagcgtc cgccagtggc cgtcttgcgt tgccgatgcc ggcgccgcgc
     1681 tgcgccggaa cgatgaacag caaacaacaa accgcctgaa caactgagca agcgctatgc
     1741 caaatagcgc gccgcgtgat ccgcatgcgc ggcgcgcaat gtggcatgga agatgcgaag
     1801 ttgcggcagg aggcttcgat gaaaatcctg ctgccgctgt gcctgtcgct gctgctgtgc
     1861 gcctgcgcgt gggtgacgcc gtatctcgac ggcacctaca acgccgccgc gtttccgccg
     1921 ctggatgcgc cggcggacag cgtggcgctg gtgggacgcg cggctgacga tgcacgcggc
     1981 gccgccgtgc tcacgctggt cgacgacatc ccgatcaagg tcaagcccga tggcagcggc
     2041 gacggccttt gggcgaagca ggtggcgctg gcgccgggtg agcacgcatt ggtcacctcg
     2101 tggcggcggc cgggcgagta ttcgccgtcg tcgtcgctgt gccaggcgga attcctcgcc
     2161 ggccaccagt acctggtgaa ttacgaagtg gccgcctccg gccgcgaggc gcgcgtgtgg
     2221 ctgagcgatg cggcggatgc gggcagcacg gtgaactgcg cggcgcgcaa accgccgccg
     2281 atgccgcaat ggccattctg agcgggatgc agccgcgcaa cctccagcga ccaggcatcg
     2341 acatgaacga gatccagcag atggtgtact cgctgcagcg cttccacatc ctcgcgctgt
     2401 acgccaacac ctcgaccgaa cgcacggtga gcgcgagcta cgcctacgcc tggtggcagg
     2461 gcatctaccc gctgctcaac gacagcgcgc catggcacaa gccgcacacg cagagtttct
     2521 ccgcgccggc gcagcaggtg gcggagctgc acgaattcct cacgcagcgc tggcgcgcgc
     2581 aggatccaat cagctttttc cagctggagg atcgctacgc catccgcgcc gccaatcgtc
     2641 ccgggccggt gtggagccag gcgcagctga tcgccgcctg ccgctacctg cacctgcacg
     2701 cgcagtacga cgaagaattc tggagtgcac tgctgggcgc cagccagagc ccgctgccgg
     2761 cggagatcat cacgcggccg gtggagccgg cggagatcca gttcgagtga tttttttcgc
     2821 caccgcaccc tgcctgtgcg cgttataaaa cattattttt ataaaaaatt aactgattat
     2881 ttataacagg cagatatagg gtgcggtgga gggtccgcgc ggcacgtgca ggtcggcaag
     2941 gccgggcacg caatccaggt cgctgcgcac cagcccgctg tcgcgcatgg ccaagggttc
     3001 gaagatgcgt tcggcgtgga tgcgttcgag cgtggtgccg gcgcagcgtt ccatggcaaa
     3061 tgacagcagc tggtagccgc cgttgcagta gatcatctgc gcgccgggcg cgaaatctgc
     3121 ggcgcgctgg cgcgtgtgat acgccagcgg cgcgccgcgc gggatcggcg agagcgcgtt
     3181 ggacaccagg gacagatcga ggtgacagcg cgtgccgccc atgtgcgtca tcagctggcg
     3241 ccgagtgggc tcgccggcct ccggcggcaa ttccggcacg tggcggccga tctgcgcgtc
     3301 gagctcgatc agcccggcct tctgcagcca gcccggcgaa gcaggtgagc tgcttgctga
     3361 tcgaggcgat gcgcatgcgc gtggccagca ggttgccggt ctgctgcgcg atgcgggcca
     3421 ggccaagcgc gcggcggtag ctgccggcgc ccgccggctc gatgccgatc accagtcgcg
     3481 gggcgtcgct gcggttccgc gggcgccgtg cacgctcgaa cgtcgcggcg ctgccgctca
     3541 gagtggagcg cagtcctgct gcgcctggaa acgatcgcgc agttcggcga gcttgccgtc
     3601 gtcggcgatc agcgcgcgga acaggatcac gccgcgctcg ccgggcgcct cgatcacccg
     3661 tgcgccggcg ggcgcctgca ccggcaggcc ggcgcgcgtg atcaccaggt cgaagtgttt
     3721 ctggccgctg gcatcggcct gcagctggct gtcgttgagc gcgaagaagt tgtcggtgtt
     3781 ggcggcgaat ccggcgatgg agaagtagct gtccggcact ggcgcctgca cgcgcagcgc
     3841 acggtcgcgc aggttgaaca cgcaggcgct gtacagcagg tccgggctcg gccgcacgat
     3901 ggcgcgtgca gaggcgtcgg cgcgcggagc gtgcaccgcg cggttgtcgc cgccggccgc
     3961 ctgcgcgatt ccgcgcatca cgcgccagct gatcgcgcgc ggcgcaaggt tggccgccag
     4021 caggttcacc gcgaccgcac tgagcagcgc cgcgatgacc gggccggtcc acttc
//
LOCUS       DASZZC010000069         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_5873,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000069 DASZZC010000000
VERSION     DASZZC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5873"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VHE37_01745"
     CDS             <1..458
                     /locus_tag="VHE37_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT34279.1"
                     /translation="TIANLESGLGNPSLTVLHKVAHALGAPIDELLASPRAKVRKWSA
                     ADISSSSRGQGVTLRPLVPEPVPEEMLSVMDLGPGATLRGTPHLPGTREYFTCLEGRV
                     LIFVAGVRNELAAGEVLAFPGNLQHAYQNPDARRAARGISVVILAKAGI"
     gene            596..958
                     /locus_tag="VHE37_01750"
     CDS             596..958
                     /locus_tag="VHE37_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34280.1"
                     /translation="MKSWLVLPAVLCALPAFASGVAMDAAVAPPLLTFDLAKIPPRTP
                     ITVTAVSADGLCSIDIDFSRMEVGAENDDSLSGRVRRAICGRQNIESGFATGSYHLLP
                     DGSAQIDSKVQVVLLVDQ"
     gene            complement(1077..3230)
                     /locus_tag="VHE37_01755"
     CDS             complement(1077..3230)
                     /locus_tag="VHE37_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HVT34281.1"
                     /translation="MHKRFTCAALAAIISVLAVQAAQATTIDNAALTGAGDGSNWPEF
                     GGQFSEQRYSPLDQVNKDNVKRLGLAWSMEVPDVWNVSSAPVEVDGTLYFAVGFSVMY
                     AVEAATGKVLWKYDPHVSRAKMRMAWGIRGMAYWNGKVYAGVQDGRLYALDAKSGALL
                     WETQTLQPGDRRYITGAPRVFNGKIIIGHGGADFGHVRGYVTAYDAETGKQIWRWYAV
                     PGNPKDGFEQPELEMAAKTWSGDWWKYGGGGTVWNAMTYDPEFNRVYLGTGNGSPWNA
                     KLRNPGGGDNLFLCSVVALDADTGKYLWHYQTNPNESWDFNSDMDMVLATLPIDGKPR
                     KLLLHAPKNGFFYVIDRETGKLVSAEKLGKVTWADHIDLATGRPVENRGVRYKNGETL
                     MWPGSGGIHNWQPMSFSPDTALTYIPAHEMAGYYDDRGIDPQKYDMDSEQYGPLGIAP
                     FFDDVPKSAGSSSLIAWDAVKQKAAWTTPTPGATNGGVMSTHGGLVFQGQADGRFVAH
                     DAATGAELWSYDMGVGTQAPPITYTVDGRQYVTVLAGWAGGHMLMGSLDAQHGWVGRN
                     HPRRVLTFVLDGKAQLPPSPPPSQPQPVDDASFQVDHALAEKGKIIYAKNCVICHGTA
                     GVAGGAAPDLRASPVPLSADSFNAVVHGGALEPRGMPVFEEFSVQDLDALRHYIRERA
                     RDKPGVWQQIKNAWHFIWLMIKQALIQRGWMSEDG"
     gene            3379..4050
                     /locus_tag="VHE37_01760"
     CDS             3379..4050
                     /locus_tag="VHE37_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34282.1"
                     /translation="MNKQKTAALALAALALGASQAASARGENFPDSYIGISGGGGDQN
                     LEVGGASSGKVSGTSYGAEGRLAFGKAFVADASYEQYKGSQNGTDVDADRMEAALGFM
                     VSVGGGGNSSLLLEGLYTGSKLKPGGADYSKHGFGIKTGFVVPVGEHFITSGIVELAH
                     IQASGGQDSQGLASFIASAGYKITRNWGIHAVYRRDAYEGRSESPGQVKFYATAYRGM
                     ISYSF"
BASE COUNT          644 a         1374 c         1331 g          720 t
ORIGIN      
        1 cgaccatcgc caacctcgag tcgggcctgg gcaatccttc gctgacggtg ctgcacaagg
       61 tggcccacgc gctcggtgcg ccgatcgacg agctgctcgc ctcgccgcgc gccaaggtgc
      121 gcaagtggag cgcggccgat atctcctcca gcagccgcgg ccagggcgtg acgctgcgtc
      181 cgctggtgcc cgagccggtg ccggaggaaa tgctcagcgt gatggacctg gggcccggcg
      241 ccaccctgcg cggcacgccg cacctgccgg gcacgcgcga atatttcacc tgcctggaag
      301 gccgcgtgct gatctttgtg gccggcgtgc gcaacgagct ggcggccgga gaggtgctcg
      361 ccttccccgg caacctgcag cacgcttacc agaaccccga tgcgcgccgc gccgcgcgcg
      421 gcatctccgt cgtcatcctg gccaaggccg gcatctgaag cgcgcttgag gaattccccc
      481 gtagtgcagg ccgcgccgcg ccgctatcgt gcgctcagga gaaagttgcg cgtccaggcg
      541 gatgacccaa tcgccggccg cgcgccatgt gccgctgctg tgtggagacg cgttggtgaa
      601 atcctggctg gtgttgccgg cggtgctctg cgccctgccc gcgtttgcgt ccggcgtggc
      661 gatggacgcc gcggtggcgc cgccgctgct caccttcgat ctcgcgaaga tcccgccgcg
      721 cacgccgatc accgttaccg cggtatccgc cgacgggctg tgttccatcg acatcgattt
      781 ctcgcgcatg gaagtgggcg cggagaatga cgactcgctg tccggccgcg tgcgccgcgc
      841 aatctgcggc aggcagaaca tcgagtccgg cttcgccacc ggctcgtatc acctgctgcc
      901 cgacggcagc gcgcagatcg acagcaaggt gcaagtggtg ctgttggtcg atcagtaggc
      961 caaaaagtct tcgaccgcac tgccattctg cctgttatta attatttact taatttaatt
     1021 ttagataata attaataacg cactaaaagg cagtgcggtc gggacaaaaa cgcatctcag
     1081 ccgtcttcgc tcatccagcc gcgctggatc agcgcctgct tgatcatcag ccagatgaag
     1141 tgccacgcgt tcttgatctg ctgccacacg ccgggcttgt cgcgcgcgcg ttcgcggatg
     1201 tagtggcgca gcgcgtcgag atcctgcacg ctgaattcct cgaacaccgg catgccgcgc
     1261 ggctccagcg cgccgccgtg caccacggcg ttgaacgagt ccgccgacag cggcaccggc
     1321 gaggcgcgca ggtcgggcgc ggcgccgccg gccacgcccg cggtgccgtg gcagatcacg
     1381 caattcttgg catagatgat cttgcccttc tccgccagcg cgtggtccac ctggaacgag
     1441 gcgtcgtcca ccggctgcgg ctgcgacggc ggcggtgacg gcggcagctg cgccttgccg
     1501 tcgagcacga aggtgagcac gcggcgcgga tggttgcgcc cgacccagcc atgctgcgcg
     1561 tcgagcgagc ccatcagcat gtggccgccg gcccagccgg cgagcacggt cacgtactgc
     1621 ctgccgtcga cggtgtaggt gatcggcggc gcctgcgtgc ccacgcccat gtcgtagctc
     1681 cacagttcgg cgcccgtggc cgcgtcgtgc gccacgaagc ggccgtcggc ctggccctgg
     1741 aacaccagcc cgccgtgcgt ggacatgacg ccgccgttgg tggcgccggg cgtcggcgtg
     1801 gtccacgcgg ctttctgctt cactgcatcc caggcgatga gcgaactgga gccggcactc
     1861 ttgggcacgt cgtcaaagaa cggtgcgatg cccagcggac cgtactgctc actgtccatg
     1921 tcgtacttct gcggatcgat gccgcggtcg tcgtagtagc ccgccatctc gtgcgcggga
     1981 atgtaggtga gcgcggtgtc gggcgagaac gacatcggct gccagttgtg gatgccgccg
     2041 gagcccggcc acatcagcgt ctcgccgttc ttgtaacgca cgccgcggtt ctccaccggg
     2101 cggccggtgg cgagatcaat gtgatcggcc caggtcacct tgcccagttt ttccgccgac
     2161 accagcttgc cggtctcgcg gtcgatcaca taaaagaagc cgttcttcgg cgcgtgcagc
     2221 aggagcttgc gcggcttgcc gtcgatgggc agcgtggcca gcaccatgtc catgtcggaa
     2281 ttgaaatccc agctttcgtt ggggttggtc tggtaatgcc agaggtactt gccggtgtcg
     2341 gcgtcgagcg cgaccaccga gcagagaaac aggttgtcgc cgccgccggg attgcgcagc
     2401 ttggcgttcc acggcgagcc gttgccggtg cccaggtaca cgcggttgaa ttccgggtcg
     2461 taggtcatgg cgttccacac ggtgccgccg ccgccgtact tccaccagtc gccgctccag
     2521 gttttcgcgg ccatctcgag ctcgggctgc tcgaagccgt ccttcgggtt gcccggcacc
     2581 gcgtaccagc gccagatctg cttgccggtc tcggcgtcgt aggcggtgac atagccgcgc
     2641 acgtggccga agtcggcgcc gccgtggccg atgatgatct tgccgttgaa cacgcgtggg
     2701 gcgccggtga tgtagcggcg gtcgccgggc tgcagcgtct gcgtttccca cagcagcgcg
     2761 ccggactttg catcgagcgc gtagagccgg ccgtcctgca cgccggcgta gaccttgccg
     2821 ttccagtagg ccatgccgcg gatgccccag gccatgcgca tcttcgcgcg agacacgtgc
     2881 gggtcgtatt tccacagcac cttgccggtg gccgcttcca ccgcgtacat caccgagaag
     2941 ccgacggcga agtacagcgt gccatccact tccaccggcg cgctcgacac gttccacacg
     3001 tcgggcactt ccatcgacca ggccaggccc aggcgcttca cgttgtcctt gttcacctgg
     3061 tccagcgggc tgtaacgttg ctcgctgaac tggccgccga actccggcca gttgctgccg
     3121 tcgcccgcgc cggtcaatgc ggcgttgtcg atggtggtgg cctgcgcggc ctgcaccgcc
     3181 agcacgctga tgatcgcggc cagcgcggcg cacgtgaatc gcttgtgcat cgtctctccc
     3241 tgaatctcgt ttgcattgat tcttgaggat gcgccggcgc gcggcctcct gcatgcgcag
     3301 tagatgctcg cttcccggca tgaagtcagc atgtacaatc gccgcggggt tgagatcaac
     3361 gaaaacgagg agacacccgt gaacaagcaa aagacggcgg cactcgctct cgcggcgctg
     3421 gcgctcggtg ccagccaggc agccagtgcg cgcggcgaga acttccccga ttcctacatc
     3481 ggcatttccg gcggcggcgg cgaccagaac ctcgaggtcg gcggcgccag cagcggcaag
     3541 gtcagcggca cttcctacgg cgccgagggg cggctggcgt tcggcaaggc gtttgtcgcc
     3601 gatgcctcgt acgaacagta caagggcagc cagaacggca ccgacgtcga cgccgaccgc
     3661 atggaagccg cgctcggctt catggtcagc gtgggcggcg gcggcaactc cagcctgctg
     3721 ctcgaaggcc tctataccgg ctccaagctc aagccgggcg gcgcggatta ttccaagcac
     3781 ggcttcggca tcaagaccgg cttcgtcgtg ccggtgggcg agcacttcat caccagcggc
     3841 atcgtcgagc tggcccatat ccaggcttcc ggcgggcagg actcgcaggg cctggccagc
     3901 ttcatcgcct cggccggcta caagatcacc aggaactggg gcatccacgc ggtgtatcgc
     3961 cgcgatgcct acgaaggccg ctccgaatcg cccggacagg tcaagttcta cgccacggct
     4021 taccgcggga tgatctcgta cagcttctga tcacctgcta cgaaaaaag
//
LOCUS       DASZZC010000070         6629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1226401,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000070 DASZZC010000000
VERSION     DASZZC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226401"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..862)
                     /locus_tag="VHE37_01765"
     CDS             complement(122..862)
                     /locus_tag="VHE37_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HVT34283.1"
                     /translation="MRSSLSCSLMAVALLCACSAQPEKPARPAAAPARAVPGQPPSPA
                     DNAIRAAQTRVDQAKKSAPGWSEGDDLLKEARAAAASGNAARAVALAHDAQHRADLAL
                     DGQYLTLSAQKLHSLYALTGLSDAQLAKMREAEVALVRGDGAHAYQLLDAIDREVKAG
                     RTHKVAPGESLWTIAGRDDVYANSLLWPLVWDANRDQVKDPNRLYQGQVLKIRPNPTV
                     DEVVRAVDYARQQMGAQVKIGEVRPAKP"
     gene            905..1504
                     /gene="wrbA"
                     /locus_tag="VHE37_01770"
     CDS             905..1504
                     /gene="wrbA"
                     /locus_tag="VHE37_01770"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HVT34284.1"
                     /translation="MSEVLVLYYSRNGSTARMARLIARGVEAGGARARLRTVPPVSAE
                     SEQVAPAVPADGPPYATHKDLKECAGLILGSPTRFGNMAAPLKYFIDGTSALWLSGDL
                     AGKPAAVFTSTGTLHGGQESTLLSMMLPLLHHGMLLVGVPYTEPALSATTAGGTPYGP
                     SHVSGVGATPRALDRNEEALCEALGRRVAQVALKLEKAS"
     gene            1501..2013
                     /locus_tag="VHE37_01775"
     CDS             1501..2013
                     /locus_tag="VHE37_01775"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVT34285.1"
                     /translation="MKLSHAALLLALFASAAYAAVEEGKPAPEFKLQDQTGKTHTLAD
                     HKGKWVVLYFYPKDDTPGCTTEACNFRDSVFKLRDAGADVLGVSLDDVKSHQAFAQKF
                     QLPFPLLADTTRETAKAYGVLTSHLGFEYAKRETFLIAPDGIVVRHWVGVDPKVHAPE
                     VLAELQRQQK"
     gene            2014..2373
                     /locus_tag="VHE37_01780"
     CDS             2014..2373
                     /locus_tag="VHE37_01780"
                     /inference="COORDINATES: protein motif:HMM:NF021369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HVT34286.1"
                     /translation="MDHRAFFQRAVLALHGLLIVAIVVGSGFGTGALLALPLAAPLPG
                     LWRGDIRTHAWSSMLLSFYAGGFLSSAYANTAHKWQSVGTAAIAALEFVSLLMYVRLK
                     AREDRLARMAASAAAAP"
     gene            complement(2291..2983)
                     /gene="hda"
                     /locus_tag="VHE37_01785"
     CDS             complement(2291..2983)
                     /gene="hda"
                     /locus_tag="VHE37_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HVT34287.1"
                     /translation="MRGHQLALPVQLRDSASFDTYYAGPNVAAVDALRAAASDVLLYG
                     APASGRSHLLQAVARVRQAAYLPLAQLQEAGADVLAGFDQAPAICLDDVDAVSGERGW
                     CTALLRLIDAQRARGSRIVLACAHAPERMDVALPDLRTRLSACAVFGLKPLSDDDRAE
                     LLRERAHARGMELAEDATRWLLAHLPRDAGSLVDALETLDRASLREQRRLTLPFVQAC
                     LRAPSAAHTSAG"
     gene            complement(2994..4115)
                     /locus_tag="VHE37_01790"
     CDS             complement(2994..4115)
                     /locus_tag="VHE37_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVT34288.1"
                     /translation="MSVTARQYLPWAIAGIALLGFLWALAPILPPFVIGAGLAYLGDP
                     IVDRLQRRGFSRTAGCVIVFAVLTVAWILALLLLVPMLYRQVIGILHAIPDFLRWLED
                     TGLPKLGITLPPDIQLDPEGFKQIIADHWSQGGGAMAGVWNSVTHSGAALVRTAAEIT
                     LIPVATFYLLRDWDDLVAWIAGMIPPRHRPLADTLARETDTVLGAFLRGQLSVMVTQG
                     ILYSIGLTLIGLKLGLLIGLLAGLVSFVPYLGFFVGVLTASAAMVAQQHEFMPVLWVL
                     LVFSIGHALEHSLLVPTLVGDKIGLHPVAVMVAVLAGAHLFGFVGILLGLPVAAVLAV
                     LLRHAKAQWLASSVFLHGATESVAAPPAQPLPGDTPPDG"
     gene            complement(4226..4825)
                     /locus_tag="VHE37_01795"
     CDS             complement(4226..4825)
                     /locus_tag="VHE37_01795"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HVT34289.1"
                     /translation="MKFLSAMSALLLAFAAAAQTFDDYQAVVPVPNQSPEARDQALRD
                     ALVSVLTQVSGTGAIAPGRIAPVLAHPETLVRSVGYETGTNGALNLIAQFTPDAVDNT
                     LKQLGLPVHGVLAGGLQDVTLRVSGISSAHGYGRVLSHLRAQPGVKNVSVLAAEGDAL
                     IATVSAEGGAARLAGALAVGGVLKRDPAEPNVMAFVLQK"
     gene            4893..5915
                     /gene="purM"
                     /locus_tag="VHE37_01800"
     CDS             4893..5915
                     /gene="purM"
                     /locus_tag="VHE37_01800"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HVT34290.1"
                     /translation="MNRKTPLTYAAAGVDIDAGDALVDDIKKIVRSTQRPEVLAGVGG
                     FGALVSLPKKYKQPILVSGTDGVGTKLKLGIETGRVEGLGQDLVAMCVNDVLVSGAEP
                     LFFLDYYATGKLEQRVAAQVIRGIAHGCKLAGAALVGGETAEMPGMYAHGDFDLAGFC
                     VAVVEKSKIIDGSRIRPGDVLIALPSSGPHSNGYSLVRKILAHHHYKLGPRGELTDAL
                     LAPTHIYVKPILRLLADKVDVRGMAHITGGGITGNLTRAFPRGTAAVVDSTSWKRPDI
                     FNWLQDKGAVEQDEMWRVFNCGVGFVCVVPRLQADRAMASLRKSKLAPWIIGEVVKGK
                     QDVRFA"
     gene            5938..6582
                     /gene="purN"
                     /locus_tag="VHE37_01805"
     CDS             5938..6582
                     /gene="purN"
                     /locus_tag="VHE37_01805"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HVT34291.1"
                     /translation="MLISGQGRNLQALIDAVAAKKIPARIACVISSRADAPGLARAQA
                     AGIPAQALPHRDYATRESYDAALAQALDRHAPEVIVLAGFMRILTAGFIARYAGRMLN
                     IHPSLLPKYPGLKTHEAALAAGDAEHGATVHYVTEQLDGGPGIIQGRLKVAPQDDAGR
                     LAERVMDEIELKIYPQAVAWQARGELCLRDGRAWLRGAPLAAPLTMADLEAEFR"
BASE COUNT         1075 a         2249 c         2310 g          995 t
ORIGIN      
        1 aaaatggatg tcggcgtgat agcaggccgc catggccggc gcgtcgcgcc gcgcgaaggc
       61 ctggtagaag cgcgtcagca gctcagtatt cgcgtgcatg cggattctcc ctggagcgcg
      121 gtcacggctt tgccggccgc acttcgccaa tcttcacctg cgcgcccatc tgctggcgcg
      181 cgtagtccac cgcgcgcacc acttcgtcga ccgtgggatt gggccggatc ttcagcacct
      241 ggccctggta gagacggttc gggtccttca cctggtcgcg gttggcatcc cacaccagcg
      301 gccacagcag cgagttggca tagacatcgt cgcggccggc aatggtccac agcgactcgc
      361 cgggcgccac cttgtgcgtc ctgccggcct tgacctcgcg gtcgatggcg tcgagcagct
      421 ggtaggcgtg cgcgccgtcg ccgcgcacca gcgccacctc ggcttcgcgc atcttcgcca
      481 gctgtgcatc cgacaggccg gtcagtgcgt agagcgaatg cagtttctgc gcagacagcg
      541 tcaggtactg cccgtcgagt gcgaggtcgg cacggtgctg ggcgtcgtgc gccagcgcca
      601 ctgcgcgggc ggcgttgccg ctggccgcgg ccgcgcgcgc ctccttcagc aggtcatcgc
      661 cctcgctcca gcccggcgcg gatttcttcg cctggtcgac gcgcgtctgc gcggcacgga
      721 tggcattgtc ggcgggcgac ggcggctgcc cgggcaccgc gcgcgccggc gccgctgcgg
      781 gccgcgcagg tttttccggc tgcgcggagc aggcgcacag cagcgcgacg gccatcaagc
      841 tgcacgagag agaggaacgc atgaatgcaa gactagaata cgcgccgcca atttggaatt
      901 ttccatgagc gaagtcctgg tgctgtacta ctcgcgcaac ggttccaccg cgcgcatggc
      961 gcggctgatc gcgcgcggcg tggaggcagg cggtgcgcgc gcgcgcctgc gcacggtgcc
     1021 gccggtgtcg gcggagagcg agcaggtggc gccggccgtg cccgccgacg ggccgccgta
     1081 tgccacgcac aaggacctga aggaatgcgc cggcctgatc ctgggcagcc ccacgcgctt
     1141 cggcaacatg gccgcgccgc tcaagtactt catcgacggc acttccgcgc tgtggctgtc
     1201 gggcgatctc gccggcaagc cggccgcggt gttcacctcc accggcacgc tgcacggcgg
     1261 gcaggagagc acgctgctgt cgatgatgct gccgctgctg catcacggca tgctgctggt
     1321 cggcgtgccg tacaccgagc ccgcgctgtc ggccaccacc gccggcggca cgccgtatgg
     1381 cccgtcgcat gtttccggcg tgggcgccac gccacgcgcg ctggaccgga atgaggaagc
     1441 gctgtgcgaa gccctcgggc ggcgcgtggc gcaggttgca ttgaaactgg agaaagcatc
     1501 atgaagctgt cgcacgccgc cctgctgctc gcgctgttcg catccgccgc ctacgccgcc
     1561 gtcgaggaag gcaagcctgc gcccgagttc aagctgcagg accagaccgg caagacgcac
     1621 acgctggctg accacaaggg caagtgggtg gtgctgtact tctacccgaa ggacgacacg
     1681 cccggctgca ccaccgaggc atgcaacttc cgcgattccg tgttcaagct gcgcgacgcc
     1741 ggcgccgatg tgctcggcgt gtcgctcgac gacgtgaaat cgcaccaggc cttcgcgcag
     1801 aaattccagt tgccatttcc gctgctggcc gacaccacgc gcgagacggc caaggcctac
     1861 ggcgtgctga cctcgcacct gggcttcgag tatgcgaagc gcgagacctt cctgatcgcg
     1921 ccggacggca tcgtggtcag gcactgggtt ggcgtcgatc cgaaagtgca cgcgccggaa
     1981 gtgctggccg agctgcagcg ccagcagaaa taaatggacc atcgggcttt cttccagcgc
     2041 gccgtgctcg cgctgcacgg actgctgatc gtcgccatcg tggtcggcag cggtttcggc
     2101 accggcgcgc tgctggcgct gccgctggcc gcgccgctgc ccggcctgtg gcgaggcgac
     2161 atacgcacgc atgcctggtc cagcatgctg ctgtcgttct atgccggcgg attcctttct
     2221 tctgcctatg ccaacaccgc gcacaagtgg cagtcggtgg gcaccgccgc catcgcggcg
     2281 ctggagttcg tcagcctgct gatgtatgtg cggctgaagg cgcgcgaaga caggcttgca
     2341 cgaatggcag cgtcagccgc cgctgctccc tgagcgaggc gcggtcgagc gtctccagcg
     2401 cgtcgaccag gcttccagca tcgcgcggca ggtgcgccag cagccagcgc gtggcgtctt
     2461 ccgccagctc cattccacgc gcatgcgcgc gctcgcgcag cagttcggcg cggtcgtcgt
     2521 cggacagcgg cttcagcccg aacaccgcgc aggcggacag ccgcgtgcgc aggtcgggca
     2581 gcgccacgtc catgcgctcg ggcgcgtgcg cgcaggccag cacgatgcgc gatccgcgcg
     2641 cgcgctgtgc atcgatcagc cgcagcagcg cggtgcacca gccgcgctcg ccgctcacgg
     2701 cgtcgacatc gtcgaggcag atcgcgggcg cctggtcgaa gccggccagc acgtcggcac
     2761 cggcctcctg cagttgcgcc agcggcaggt acgccgcctg gcgcacgcgc gccaccgctt
     2821 gcagcaggtg gctgcgcccg gaggcgggcg cgccgtagag cagcacgtcg gacgcagcgg
     2881 cgcgcagcgc gtcgaccgcg gccacattcg ggccggcgta gtaggtgtcg aagctggccg
     2941 agtcgcgcaa ttgcaccggc agcgcgagct ggtgtccgcg catgaaaaaa ttcctagccg
     3001 tcaggcggcg tatcgccggg gagcggctgc gccggcggcg ctgctacgga ttccgtagca
     3061 ccgtgcagga acaccgacga cgccagccac tgcgccttgg cgtggcgcag cagcaccgcg
     3121 agcaccgccg ccaccggcag gccgagcagg atgccgacga agccgaacag gtgcgcgccg
     3181 gccagcaccg ccaccatcac cgccaccggg tgcaggccga tcttgtcgcc gaccagcgtg
     3241 ggcaccagca ggctgtgctc cagcgcgtgg ccgatgctga acaccagcag cacccacagc
     3301 accggcatga actcgtgctg ctgcgccacc atcgctgcgc tggcggtgag cacgccgacg
     3361 aagaatccga gatacggcac gaagctgacc aggccggcga gcaggccgat gagcaggccg
     3421 agcttgaggc cgatcagcgt gaggccgatg gagtagagga tgccctgcgt caccatcacc
     3481 gacagctgcc cgcgcaggaa ggcgccgagc acggtgtcgg tctcgcgtgc cagcgtatcg
     3541 gccagcgggc ggtggcgcgg cgggatcatg ccggcgatcc acgccaccag gtcgtcccag
     3601 tcgcgcagca ggtagaaggt ggcgaccggg atcagcgtga tctccgccgc cgtgcgcacc
     3661 agcgccgcgc ctgaatgcgt caccgagttc cacacgccgg ccatggcgcc gccgccctgg
     3721 ctccagtggt cggcgatgat ctgcttgaat ccctccgggt cgagctggat gtccggcggc
     3781 agcgtgatgc cgagcttggg caggccggta tcctcgagcc agcgcaggaa atccgggatg
     3841 gcgtgcagga tgccgatgac ctggcgatag agcatcggta ccagcagcag cagcgcgagg
     3901 atccacgcca ccgtcagcac cgcgaacacg atgacgcagc cggcggtgcg cgagaagccg
     3961 cggcgctgca ggcggtcgac gatggggtca ccgagatagg ccagtcccgc accaatcacg
     4021 aacggcggca ggatcggcgc cagcgcccac aggaaaccga gcagcgcaat gccagcgatg
     4081 gcccacggca ggtactggcg cgcagtgacg ctcattttcc gctccaccgc atgcgggttt
     4141 ttgatgttat taaatattgc atgaaaatat ttttatgaga taaataataa catcgaaaat
     4201 gccagtgcgg tggaggcccg cgcggctatt tttgcagcac gaaggccatc acgttgggct
     4261 cggccggatc gcgcttgagc acgccgccca ccgccagcgc gccggccagc cgcgcggcgc
     4321 cgccttccgc gctcacggtg gcgatcagcg cgtcgccttc ggccgccagc acgctcacgt
     4381 tcttcacgcc cggctgcgcg cgcaggtgcg acagcacgcg gccgtagccg tgcgcggacg
     4441 agatcccaga cacgcgcagc gtcacgtcct gcaggccgcc ggcgagcacg ccgtgcaccg
     4501 gcaggccgag ctgcttaaga gtgttgtcga ccgcgtcggg cgtgaactgc gcgatcaggt
     4561 tgagtgcgcc attggtgccg gtttcgtagc ccacgctgcg caccagcgtc tcggggtgtg
     4621 ccaaaacggg cgcgatgcgc ccgggcgcga tggcgcccgt gcccgacacc tgggtgagca
     4681 cgctgaccag cgcgtcgcgc aatgcctggt cgcgcgcctc cgggctctgg ttgggcaccg
     4741 gcaccacggc ctggtagtcg tcgaaggtct gcgccgcggc ggcgaaggcg agcagcagcg
     4801 cggacatcgc ggagagaaat ttcatgcgcg aattatcggg ggcggggcgg taaaaggtaa
     4861 aatgatttca ctcccgctac cgactctcgc gcatgaacag gaaaacgccg ctgacctacg
     4921 cggctgcagg tgtggacatc gacgccggcg acgcgctggt cgacgacatc aagaagatcg
     4981 tcaggagcac gcagcggccg gaagtgctgg ccggcgtggg cggcttcggc gcgctggtgt
     5041 cgctgcccaa gaaatacaag cagccgatcc tggtgtcggg caccgacggc gtcggcacca
     5101 agctgaaact gggcatcgag accggccgcg tcgaagggct cggccaggac ctggtcgcca
     5161 tgtgtgtcaa cgacgtactg gtgtcgggcg ccgagccgct gttcttcctc gactactacg
     5221 ccaccggcaa gctggagcag cgtgtggcag cgcaggtcat ccgcggcatc gcgcacggct
     5281 gcaagctggc cggcgcagca ctggtcggcg gcgaaaccgc ggagatgccg ggcatgtacg
     5341 cacacggcga tttcgacctg gctggcttct gcgtggcggt ggtggagaaa tcgaaaatca
     5401 tcgacggctc gcgcatcagg ccgggcgacg tgctgatcgc gctgccgtcg tccggcccgc
     5461 attccaacgg ctactcgctg gtgcgcaaga tcctggcgca ccatcactac aagctggggc
     5521 cgcgcggcga actcaccgac gcgctgctgg cgcccaccca catctacgtg aagccgatcc
     5581 tcaggctgct ggccgacaag gtcgacgtgc gcggcatggc gcacatcacc ggcggcggca
     5641 tcaccggcaa tctcacccgc gcgtttccca ggggcaccgc agcggtggtc gacagcactt
     5701 cgtggaagcg gcccgacatc ttcaactggc tgcaggacaa gggcgcggtg gagcaggacg
     5761 agatgtggcg cgtgttcaac tgcggcgtcg gcttcgtctg cgtggtgccg cggctgcagg
     5821 ctgaccgcgc catggcctca ctgaggaaat cgaagctggc gccgtggatc atcggcgagg
     5881 ttgtgaaggg aaaacaggac gtgcgctttg cctgagggcg ctacgaaaaa cgtagcggtg
     5941 ctgatttccg gccagggccg caacctgcag gccctgatcg acgcggtcgc cgcgaagaag
     6001 atccccgcgc gcatcgcctg cgtcatcagc agccgcgccg acgcgcccgg cctggcgcgt
     6061 gcgcaggctg cgggcatccc ggcgcaggcg ctgccgcacc gcgattacgc cacgcgcgag
     6121 tcctacgacg ccgcgctggc gcaggcgctg gatcgccacg cgccggaggt catcgtgctg
     6181 gccggcttca tgcgcatcct caccgccggc tttatcgcgc gctacgccgg acgcatgctg
     6241 aacattcatc cttcgctgct gcccaagtac ccggggctga agacgcacga ggctgcgttg
     6301 gccgccggcg acgccgagca cggcgccacc gtgcattacg tgaccgagca gctcgacggc
     6361 gggccgggca tcattcaggg ccggttaaag gtggcgccgc aggatgatgc gggcaggctg
     6421 gcggagcgtg tgatggatga aatcgaattg aagatctacc cgcaggcggt ggcgtggcag
     6481 gcgcgcggtg agctgtgcct gcgcgacggc cgcgcctggc tgcgcggcgc gccgctggcg
     6541 gcgccgctga ccatggccga cctggaagcg gagttccgct gatgcgtgcg ttggccctgc
     6601 tcctgcttgt tgttgccgcc agcgcggct
//
LOCUS       DASZZC010000071         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_713094,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000071 DASZZC010000000
VERSION     DASZZC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713094"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VHE37_01810"
     CDS             <1..435
                     /locus_tag="VHE37_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HVT34292.1"
                     /translation="DVTVQAQVLQLLAGLRREFGLSLLLITHDLGVMAQVCDRALVMY
                     AGRCVEHGTAAELLAAPRHPYTRGLLNSRPRLDRPVSRRLSAIAGSAANAAAGGCAFA
                     PRCPSRAAACSASLPLEQSGSAGGFSCHFPLPGANAVAPAEK"
     gene            506..>2095
                     /locus_tag="VHE37_01815"
     CDS             506..>2095
                     /locus_tag="VHE37_01815"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT34293.1"
                     /translation="MDNKARSHLRTASWLVAGCAALVSLGASALEFNIPVNSDNTIDG
                     VLNTTITVGGAWRMHSPSTDLIGKADLNPDVCGRAANGKPLYQSCQGLFREQALPAYH
                     LSQAPGQFTMNGDDGNLNYGRGAPTSGIAKVTQDISLKWGDFGFFAKALYFYDWVNNN
                     FTENHPNYVTKDNYLQVGAVSSLATDTLPRSDSRPCGSRNPTIVGPCGIVYGKGAQVY
                     SKRTDGETLKEIGTDLQPLDYFFYGKLPLPFDKQLTFKIGSQSVNWGESTLLAFGSIN
                     QANPINANNFYRIGGQVEEIFRPSSMLFLSTEPFEGTTIEGYYQLEWIPLEAPAPGSF
                     LSFLDLGPKNAGNNWANLSFGGAADDPDIDPNAMMPNAYASQFIPSHGGYARLQDNSL
                     SGLTNTSLRAERLRDNEPNSSGQFGLALKYYADWLNNGTELGFYFEKYHSRIPFVSVY
                     STVASCARPEGNNLGINPTNTATFFAACKNMPIEHSLAGLGVPSGLPAATQQVLDIFN
                     GPSGIFTQLGLGTNLPVANDVY"
BASE COUNT          389 a          721 c          638 g          347 t
ORIGIN      
        1 gacgtcaccg tgcaggcgca ggtgctgcag ctgctcgccg ggctgcgccg cgagttcggc
       61 ctgagcctgc tcctgatcac gcatgacctg ggcgtgatgg cccaggtctg cgaccgcgcg
      121 ctggtcatgt acgcagggcg ctgtgtggaa catggcacgg cggctgagct gctggctgcg
      181 ccgcgccacc cgtatacgag aggcctgctc aattcccggc cacggctgga ccggccggtg
      241 agccggcggc tgagtgccat cgcgggttcg gcggcgaacg ccgccgctgg cggctgcgcg
      301 ttcgccccgc gctgtccttc ccgcgccgcg gcctgcagcg cgagcctgcc gctggagcaa
      361 tccggctccg cgggcggatt cagctgccac tttcccctgc cgggtgctaa tgctgttgcg
      421 cctgctgaga aatagctgat aatttcctga tccgagacaa aattgaccaa cacccggcgg
      481 cggcggccgt catcaggggg agaaaatgga caacaaggca agaagccacc ttcggaccgc
      541 aagctggctc gtcgccggct gcgccgcact ggtcagcctg ggcgcgagcg cgctggaatt
      601 caacataccc gtcaacagtg acaacaccat cgacggagtg ctcaacacca ccatcacggt
      661 gggcggagcc tggcgcatgc actcgccgag caccgacctg atcggcaagg ccgacctcaa
      721 tccagatgtc tgcggccgcg ccgccaatgg caaaccgctg taccagtcct gccagggcct
      781 gttccgcgag caggcgctac cggcctatca cctgtcgcag gcgccgggcc agttcaccat
      841 gaacggcgac gatggcaacc tgaattatgg ccgcggcgcg ccgacctcgg gcatcgccaa
      901 ggtcacgcag gacatctcgc tcaagtgggg cgacttcggc ttcttcgcca aggcgctgta
      961 tttctatgac tgggtgaaca acaacttcac cgagaaccac ccaaactacg tcaccaagga
     1021 caactacctg caggtgggcg cggtctcgag cctggccact gatacgctgc cgcgcagcga
     1081 ttcgcgtccc tgcggttcgc gcaacccgac catcgtcggt ccctgcggca tcgtctacgg
     1141 caagggcgcg caggtgtaca gcaagcgcac tgatggcgag acgctgaagg aaataggcac
     1201 cgacctgcag ccgctggatt acttcttcta cggcaagctg ccgctgccgt tcgacaagca
     1261 gctgaccttc aagatcggca gccagagcgt gaactggggc gaatccacgc tgctcgcctt
     1321 cggctcgatc aaccaggcca acccgatcaa cgccaacaac ttctaccgca tcggcggcca
     1381 ggtggaagaa atcttccgcc cgagcagcat gttgttcctg tcgaccgagc cgttcgaagg
     1441 caccaccatc gaaggctatt accagctcga gtggatcccg ctggaagcgc cggcgccggg
     1501 ctcgttcctg tcgttcctcg atctgggtcc gaagaacgcg ggcaacaact gggcgaacct
     1561 ctcgttcggc ggcgcggccg acgatccgga catcgacccc aacgcgatga tgcccaatgc
     1621 ctacgccagc cagttcatcc cttcgcacgg aggctatgcg cgcctgcagg acaactcgct
     1681 gtcgggcctg accaacacct cgctgcgcgc cgagcgcctg cgtgacaacg agccgaattc
     1741 ctccggtcag ttcggcctcg cgttgaagta ctacgccgac tggctgaaca acggcaccga
     1801 gctgggcttc tacttcgaga agtaccactc gcgcattccg ttcgtcagcg tgtattccac
     1861 cgtggccagc tgcgcgcggc ccgagggcaa caacctgggg atcaatccga ccaacaccgc
     1921 caccttcttc gcggcctgca agaacatgcc gatcgagcat tcgctcgccg gcctcggcgt
     1981 gccgtcggga ctgccggcgg cgacgcagca ggtgctggat atcttcaacg ggccgagcgg
     2041 catcttcacc cagctgggcc tgggcaccaa cctgccggtg gccaacgatg tctat
//
LOCUS       DASZZC010000072         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1160360,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000072 DASZZC010000000
VERSION     DASZZC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160360"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VHE37_01820"
     CDS             <1..397
                     /locus_tag="VHE37_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34294.1"
                     /translation="EPVAVQAVLLLFRGLGRLLADPRMAIGIAGYWLIPDKPETAGIR
                     EAQRTFEQNGKALIAEVLRRGQAQGRLDLAGDLDDVARAMISLVEAVVLPLRHQTPDE
                     VHRTLGVLARSFFRAYASAADLLQKTREI"
     gene            complement(521..1873)
                     /locus_tag="VHE37_01825"
     CDS             complement(521..1873)
                     /locus_tag="VHE37_01825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HVT34295.1"
                     /translation="MNQNWQLDSWQARPAAQHPQYPDAAALAAALAELKRLPPLVTSW
                     EVDALRAQLAQAQEGTRFVLQGGDCAESFADCEPGLITNRLKVLLQMSLVLVHGLKKR
                     VVRIGRFAGQYAKPRSADNETRDGVTLPSYRGDIINAPEFTAEARRLDPQRLLRAHAR
                     SAMTINFVRGLIDGGFADLHHPEYWDLDWVAHSHMAADYQRMVDSIGDSDRFMETLAG
                     APVGDFSRVDFYTSHEALSLWYEQAQTRTVPRKQGWYNLSTHFPWIGMRTAAADGAHV
                     EYVRGLRNPVGVKVGPAMSAEWLLELCRILNPAEEPGRLTLIHRMGNAHIGQHLPRLI
                     DAVRGAGHRVLWLCDPMHGNTQTAANGLKTRRFEDIRGELTQAFEIHAACGSRLGGVH
                     LELTGENVTECTGGARNLIDSDLERAYRSQVDPRLNYEQALELAMQISRMAGGKSARS
                     "
     gene            1933..>3020
                     /locus_tag="VHE37_01830"
     CDS             1933..>3020
                     /locus_tag="VHE37_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650086.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HVT34296.1"
                     /translation="MTASPFSEHTPLMQQYLSIKARHADTMVLFRMGDFYELFYDDAR
                     KAARLLNITLTQRGESAGAPVVMAGVPHHSAEQYLARLIKLGESVVIAEQVGEVGAEK
                     GPVKREVTRIITPGTATEESLLDPRRQNLLAAVCSAGGRYGLAWLELSSGRFSVMECA
                     GSNDLAGELQRLSPAELLHAEGLGADLSAHAARARPAWQFDHASAYRLLTEQLRTRDL
                     RGYGCEDLPAAVAAAGALLQYVQETQRGVLPHVRALRVERHDEALVLDAVTRRNLEID
                     HARSDDTRHTLLGVMDRCVSAMGSRALARWLLRPLRDRAALRARYQAIARLLDSGAHA
                     ALREALREISDLERIVARIALRSARPRDL"
BASE COUNT          471 a         1059 c         1003 g          487 t
ORIGIN      
        1 ggaacccgtc gctgtgcagg ccgtactgct gctgttccgc ggcctcggac gcctgctggc
       61 cgatccacgc atggccatcg gcatcgccgg ctactggctg attccggaca agccggaaac
      121 cgccggcatc cgcgaggcgc agcgcacctt cgagcagaac ggcaaggcgc tgatcgccga
      181 ggtgctgcgc cgcggccagg cgcagggacg gctggacctc gccggcgatc tcgacgacgt
      241 ggcgcgcgcc atgatctcgc tggtggaagc cgtggtgctg ccgctgcggc accagacgcc
      301 ggacgaagtg catcgcacgc tcggcgtgct cgcgcgctcg tttttccgcg cctacgccag
      361 cgccgcggac ctgctgcaga agacgcggga gatctgaaaa cgcccgccgc agcctccacc
      421 gcaccggccg aaagcatgtt gctatttatc gtatatattt ataataaaca aataattaat
      481 aacaacaaaa aaggcgatgc ggtggacctg ttttcagccc tcaactgcgc gccgatttgc
      541 cgcccgccat ccgggaaatc tgcatcgcca gctccagcgc ctgctcgtag ttgaggcgcg
      601 ggtccacctg cgagcgatac gcgcgctcga ggtcgctgtc gatgaggttg cgcgcgccgc
      661 cggtgcactc ggtcacgttc tcgccggtga gttcgaggtg cacgccaccc aggcgcgagc
      721 cgcaggccgc gtggatttcg aaagcctgcg tcagctcgcc gcggatgtcc tcgaagcgcc
      781 gcgtcttcag cccgttggcc gcggtctgcg tgttgccgtg catgggatcg cacagccaca
      841 gcacgcggtg ccccgcgccg cgcacggcgt cgatcagccg cggcaggtgc tgcccgatgt
      901 gcgcgttgcc catgcgatgg atcagcgtga gccgccccgg ctcttcagcg ggattgagga
      961 tacggcagag ttccagcagc cattcagcgc tcatcgccgg ccccactttc acgccgaccg
     1021 ggttgcgcag gccgcgcacg tattccacgt gcgcgccgtc ggccgcggcg gtgcgcatgc
     1081 cgatccacgg gaaatgcgtc gacaggttgt accagccctg cttgcgcggc accgtgcgcg
     1141 tctgcgcctg ctcgtaccac agcgagagcg cctcgtgcga ggtgtagaaa tccacgcgcg
     1201 agaaatcgcc gaccggcgcg ccggccagcg tttccatgaa gcggtcggaa tcgccgatgc
     1261 tgtcgaccat gcgctggtaa tccgccgcca tgtgcgaatg cgccacccag tccagatccc
     1321 agtattccgg gtgatgcagg tcggcgaaac cgccatcgat caggccgcgc acgaagttga
     1381 tggtcatggc cgagcgcgca tgcgcgcgca gcagccgctg cggatcgagc cggcgcgctt
     1441 cggcggtgaa ttccggcgca ttgatgatgt cgccgcggta gctcggcagc gttacgccat
     1501 cgcgggtctc gttgtcggct gagcgcggct tggcgtactg gccggcgaag cggccgatgc
     1561 gcaccacgcg cttcttcagg ccgtgcacca gcaccagcga catctgcagc agcaccttga
     1621 ggcggttggt gatcaggccc ggctcgcagt cagcgaagct ctctgcacag tcgcccccct
     1681 gcagcacgaa gcgcgtgcct tcctgcgcct gcgccagctg tgcgcgcagc gcgtcgactt
     1741 cccacgacgt gaccagcggc ggcaggcgct tgagctcggc cagcgcagcc gccagggcgg
     1801 cggcatcggg atattgcggg tgttgcgcgg caggcctggc ctgccacgaa tccagttgcc
     1861 agttttggtt cacgcgaaac tcggccagcg gcgataatgg agcgtcagta taaagctccg
     1921 ccccgcccgc ccatgaccgc ctctcccttc agcgagcaca cgccgctgat gcagcagtac
     1981 ctgtcgatca aggcgcgcca tgccgacacg atggtgctgt tccgcatggg cgatttctac
     2041 gagttgttct acgacgacgc acgcaaggcg gcgcgcctgc tcaacatcac gctgacccag
     2101 cgcggtgaat cggccggagc gccggtggtg atggccggcg tgccgcatca ctcggcggag
     2161 caatacctgg cgcggctgat caagctgggc gaatcggtgg tgatcgccga acaggtgggc
     2221 gaagtcggcg ccgagaaagg accggtgaag cgcgaggtca cgcgcatcat cacgcccggc
     2281 accgccaccg aggaatcgct gctcgacccg cggcggcaga acctgctcgc cgcggtctgc
     2341 tccgccggcg ggcgctacgg cctcgcctgg ctggagcttt cttccgggcg tttctcggtg
     2401 atggaatgcg ccggcagcaa cgaccttgcc ggcgaactgc agcgcctttc tccggccgaa
     2461 ctgctgcatg ccgaagggct gggcgccgat ctctcggcgc acgccgcgcg ggcacggccg
     2521 gcctggcagt ttgaccacgc gtcggcctac cgcctgctca ccgagcagct gcgtacgcgc
     2581 gacctgcgcg gctacggctg cgaagacctg cctgcggccg ttgccgcagc cggcgccctg
     2641 ctgcagtacg tacaggaaac ccagcgcggc gtgctgccac atgtgcgcgc gctgcgcgtg
     2701 gagcggcacg atgaagccct ggtgctcgac gccgtcaccc ggcgcaacct ggagatcgat
     2761 cacgcgcgct ccgacgacac ccggcacacc ctgctcggcg tgatggatcg ctgcgtcagc
     2821 gccatgggtt cgcgcgccct ggcccgctgg ctgctgcggc cgctgcgcga ccgtgcggcc
     2881 ctgcgcgcgc gctaccaggc cattgcacgc ctgctcgact ccggcgctca tgcagcgctg
     2941 cgcgaggcgc tgcgcgaaat ttccgacctc gagcgcatcg tcgcccgcat tgcgctgcgc
     3001 tccgcgcgcc cgcgcgacct
//
LOCUS       DASZZC010000073         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1226991,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000073 DASZZC010000000
VERSION     DASZZC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226991"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VHE37_01835"
     CDS             <1..363
                     /locus_tag="VHE37_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34297.1"
                     /translation="GVLMAINNNALRGLSRDNADHPCQEHVARVEAALADHPGYHSQR
                     VYSCPDGGSDACHVSLLVTAPDQQTYVVDNGAVLDDAHFRASVAHLDEYAAQLDNIYW
                     IGALPDHARQIADARGRF"
     gene            646..1194
                     /locus_tag="VHE37_01840"
     CDS             646..1194
                     /locus_tag="VHE37_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34298.1"
                     /translation="MRIKFLAGVAVAVMMLQGCASTAELEQRQLAYTGTGSNPCLFGG
                     VTEAANLHCQQVRWGSEGVLMAINAQATRGLSRDDADHPCQEHVKRVETALADHPGYR
                     SQRVYSCPAGGSDNCHVSLLVSAPDHKMYVVDNGAVLKDDRYRGNVAALQEYAAELDQ
                     VYWVGSMPANAKQIANARYSGL"
     gene            complement(1254..>2268)
                     /gene="prpC"
                     /locus_tag="VHE37_01845"
     CDS             complement(1254..>2268)
                     /gene="prpC"
                     /locus_tag="VHE37_01845"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HVT34299.1"
                     /translation="ADSSEFEEIAHLLVHGKLPNAAELAGYKAKLKSLRGLPANVMQA
                     LEALPASAHPMDVMRTGVSALGCNLPEKDDHNHPGARDIADKLMASLGSMLLYWYHYA
                     HNGKQIEVETDDDSIGGHFLHLLHGRKPPESWVKAMHTSLILYAEHEFNASTFTCRVV
                     AGTGSDMYSAICGGIGALRGPKHGGANEVAFEIQKRYDTPDEAEADIKARVERKEVII
                     GFGHPVYTVSDPRNKVIKGVAQRLSAEQKNMKMYNIAERLETVMWDIKKMFPNLDWFS
                     AVSYHMMGVPTAMFTPLFVIARTSGWSAHIIEQRIDGKIIRPNANYVGPEDQKFVPLN
                     KRT"
BASE COUNT          375 a          742 c          741 g          410 t
ORIGIN      
        1 ggcgtgctga tggcgatcaa caacaacgcg ctgcgcgggt tgtcgcgcga caacgccgat
       61 catccatgcc aggagcacgt ggcgcgcgtg gaagccgcgc tggcggatca tcccggctac
      121 cactcgcagc gcgtgtattc ctgcccggac ggcggctccg atgcctgcca cgtcagcctg
      181 ctggtgacgg caccggatca gcagacctac gtggtcgaca acggcgcggt gctcgacgat
      241 gcgcatttcc gcgccagcgt tgcgcatctg gacgagtacg ccgcgcagct cgacaacatc
      301 tactggatcg gcgccctgcc cgaccacgca cggcagatcg ccgacgcgcg cggccgcttc
      361 tgaacactgt tcaccgcctg tcccccgaca ggcggtgacg gctccggcgg catttttgcg
      421 acgccccgca ccgccggtca tcggccgaaa accggcattg ccggaacttg cgaccgccgg
      481 tcggacaact gacctcactg atcgcccgca agcgcggctg gcgcaaggag cagcaggagt
      541 ttttgccggg tatctggcgc cgcttccgat ccctagattg ccgccgtcgg aaatttccta
      601 catcgcgtag gagaaacggc aagtcaacgt aaccaaggag cggcaatgcg tatcaagttt
      661 ctggcgggtg tagcggtggc ggtgatgatg cttcagggat gtgcgtcaac cgcggagctg
      721 gagcagcgcc agctcgccta caccggcact ggcagcaacc cctgcctgtt cggcggcgtg
      781 accgaggcgg ccaacctgca ctgccagcag gtgcgctggg gcagcgaggg cgtgctgatg
      841 gcgatcaacg cccaggccac ccgcgggctg tcgcgcgacg acgccgatca tccgtgccag
      901 gaacacgtca agcgcgtgga aaccgcgctg gccgatcacc ccggctaccg ctcgcagcgc
      961 gtctactcct gcccggccgg tggctccgac aactgccatg tgagcctgct ggtgtcggcg
     1021 ccagaccaca agatgtacgt ggtcgacaac ggcgcggtgc tgaaggacga ccgctatcgc
     1081 ggcaacgtcg ccgcgctgca ggaatacgcc gccgagctgg accaggtgta ctgggtcggc
     1141 agcatgccgg ccaatgccaa gcagatcgcc aacgcgcgct acagcgggct ctgatcaggt
     1201 ttctggggcg ggaacgaaaa cgccggcgcg agccggcgta tttttattcc cgttcaggtc
     1261 cgcttgttca gcggcacgaa cttctggtct tccggcccca cgtaattggc attcggccgg
     1321 atgatcttgc cgtcgatgcg ctgctcgatg atgtgcgcac tccacccgct ggtgcgcgcg
     1381 atgacgaaca gcggcgtgaa catggcggtg ggcacgccca tcatgtggta gctcaccgcg
     1441 ctgaaccagt cgaggttggg gaacatcttc ttgatgtccc acatcaccgt ctccaggcgc
     1501 tcggcgatgt tgtacatctt catgttcttc tgctcggccg acaggcgctg ggccacgccc
     1561 ttgatcacct tgttgcgcgg atcgctcacc gtatacaccg ggtggccgaa gccgatgatc
     1621 acttccttgc gctcgacgcg cgccttgatg tcggcctcgg cctcatcggg agtgtcgtag
     1681 cgcttctgga tctcgaaggc cacttcgttg gcgccaccgt gcttgggccc gcgcagcgcg
     1741 ccaatgccgc cgcagatggc gctgtacatg tcggagccgg tgccggcgac gacgcggcag
     1801 gtgaaggtgg acgcgttgaa ctcatgctcg gcgtagagga tcagcgaggt gtgcatcgcc
     1861 ttgacccagc tttccggcgg cttgcgcccg tgcaacaggt gcaggaagtg gccaccgatg
     1921 gaatcgtcat cggtttcgac ctcgatctgc ttgccgttgt gcgcgtagtg gtaccagtac
     1981 agcagcatcg agccgagcga ggccatcagc ttgtcggcga tgtcgcgcgc gccgggatgg
     2041 ttgtggtcat ccttctccgg caggttgcag cccagcgcgg acacgccagt gcgcatcacg
     2101 tccatcgggt gcgcgctcgc cggcagcgct tccagcgcct gcatcacgtt ggccggcagg
     2161 ccgcgcagcg atttcagctt ggccttgtag cccgcgagtt ccgcggcatt gggcagcttg
     2221 ccgtggacca gcaggtgcgc gatctcttcg aattcgctgg agtcggcg
//
LOCUS       DASZZC010000074         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_774199,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000074 DASZZC010000000
VERSION     DASZZC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774199"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2263)
                     /locus_tag="VHE37_01850"
     CDS             complement(<1..2263)
                     /locus_tag="VHE37_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34300.1"
                     /translation="MAWLNAGSINAQNVDTHNLGGLFFTNDPSVIGSGGSPINATPGA
                     GMYIAASGRTRASTMRIYAHDDAGGDIGFGVVADASSGVEAETDGAIVDVIPGFLTQF
                     NHPLSGEGGGSGGNGIPADLALPANDITAQQFSIRFGKASTLADSILTATGSPANDSG
                     FIDINNDSSLQGQRIALDVGGSSTWTGNQVTVAHSKISGGSLTINANGVMDNGTDYPG
                     GAQIEDATLNLDTLLVQANDAGILIEGDTSTPSGNASLVHAHMLTLDATDKVDLANAR
                     LAYTNGTITGDKVTVDNSLLTGLHDPETDAEDQLTLTGHGLNHGIAVQFQNGGAINAS
                     NATLVADHGLISLDNASVTVHADVSISANDGNIELLDNSVMNYNTAELTAMGHAGLDQ
                     NDNPITVGGTITIGSSTASSSRTDAVFNSGGKTTLTAYGISLDHSTIAGGNLTATADK
                     FVDARHSVFSGTGTDLTAHGARNGAGINLVADDFEYDNHVGLHADQAIAVADESGATG
                     GTSSISAPLVLLESGAAVGLTNTGASGDRFVVRGVNGNAQAGSFSASNSNLSFGSSIT
                     IDALGLAEIDGGQLNSIAGYGGGPADGTVTLRAGSVQINGATIDGSHATRITAGLSAP
                     GGARAATTASGDLDLSGTIGDSGSYVLLAAAGNVAQNGTLTINANALDVASHNGFIDL
                     GTSVINVGGGTAVGGHDHALQQNLDSDGLFPASPGPNAAFQAAQDIALGTLNFTTAGH
                     YLYVSAGSAPPVGS"
BASE COUNT          306 a          697 c          840 g          433 t
ORIGIN      
        1 cgctgccgac cggcggtgcg ctgccggcgc tgacgtacag gtaatggccg gcggtggtga
       61 agttgagggt gcccagcgcg atgtcctgcg ccgcctggaa cgccgcgttc ggacccgggc
      121 tggcggggaa caggccatcc gagtccagat tctgctgcag cgcatggtcg tgtccaccca
      181 cagcggtgcc gccgccgacg ttgatgaccg atgtgccgag gtcgatgaag ccgttgtggc
      241 tggccacatc cagcgcgttg gcgttgatgg tcagcgtgcc gttctgcgcc acgttgccgg
      301 cggccgccag cagcacgtag ctgccgctgt cgccgatggt tccgctcagg tcaaggtcgc
      361 cgctcgcggt ggtggccgca cgcgcgccgc ccggggcgct caggccagcg gtgatacgcg
      421 tggcatgcga gccgtcgatg gtggcgccat tgatctgcac cgagcctgca cgcagggtga
      481 cggtgccgtc ggctgggccg ccgccgtagc cggcgatcga attcagctga ccgccgtcga
      541 tctcggccag tcccagtgcg tcgatggtga tgctggaacc gaacgacagg ttggagttgc
      601 tggcgctgaa cgagccggcc tgcgcgttgc cgttgacgcc gcgcaccacg aagcggtcgc
      661 cgctggcgcc ggtgttggtc aggccgaccg ctgcgccgga ttccagcagc accagcggcg
      721 cagagatcga cgaagtaccg ccggtggcgc cgctctcgtc ggccaccgcg atggcctggt
      781 cggcatgcag gccgacatgg ttgtcatact cgaaatcatc cgcgaccagg ttgatgccgg
      841 cgccgttcct ggcgccgtgc gcggtgagat ccgtgccggt gccgctgaaa acggagtggc
      901 gggcgtcgac gaacttgtcc gcggtcgcgg tcaggttgcc gccggcgatg gtgctgtggt
      961 cgaggctgat gccgtacgcg gtcagcgtgg tcttgccgcc ggagttgaag accgcgtcgg
     1021 tgcggctgct cgaggcagtg ctgctgccga tggtgatggt gccgccgacg gtgatcgggt
     1081 tgtcgttctg gtccagcccg gcgtggccca tggcggtcag ttccgccgtg ttgtagttca
     1141 tcaccgagtt gtcgagcagc tcgatgttgc cgtcgttggc gctgatgctc acgtccgcat
     1201 gcacggtgac cgaggcattg tcgagcgaga tcagtccatg gtcggcgacc agcgtggcgt
     1261 tgctcgcatt gatggcgccg ccgttctgga actgcacggc gatgccgtgg ttaaggccat
     1321 ggccggtgag ggtcagctga tcctcggcat cggtttccgg atcgtgcagg ccggtgagca
     1381 ggctgttgtc caccgtgacc ttgtcgcccg tgatggtgcc gttggtatag gccaggcgcg
     1441 cgtttgccag gtccaccttg tcggtggcgt ccagggtcag catgtgcgca tgcaccagtg
     1501 aggcattgcc ggacggcgtg ctggtgtcgc cctcgatcag gatgccggca tcgttggcct
     1561 gcaccagcag cgtgtccagg ttgagcgtgg catcttcgat ctgcgcgccg cccgggtaat
     1621 cggtgccgtt gtccatcacg ccgttggcat tgatggtcag gctgccgccg ctgatcttgc
     1681 tgtgggccac ggtgacctgg ttgccggtcc aggtgctgct gccgccgacg tccagcgcga
     1741 tgcgctggcc ctgcagcgag ctgtcgttgt tgatgtcgat gaagcccgag tcattcgcgg
     1801 gtgagccggt agccgtcagg atgctgtcgg ccagggtcga agccttgccg aagcggatgg
     1861 agaactgctg cgcggtgatg tcgttggcgg gcagggcaag atcggccggg atgccgttgc
     1921 cgccggagcc gccgccttcg cccgacagcg gatggttgaa ctgggtgagg aagcccggga
     1981 tgacgtcgac gatggcgccg tcggtctccg cttccacgcc gctgctggca tccgccacca
     2041 cgccgaagcc gatgtcgccg ccggcgtcgt catgcgcgta gatgcgcatg gtgctggcgc
     2101 gagtccggcc gctggcggcg atgtacatgc ccgcgcccgg cgtggcgttg atcggcgatc
     2161 cgccgctgcc gatgacggag ggatcgttgg tgaagaacag cccgccgaga ttgtgggtgt
     2221 cgacattctg cgcattgatc gagccggcgt tgagccaggc cacggtgtag ccgtaa
//
LOCUS       DASZZC010000075         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1098788,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000075 DASZZC010000000
VERSION     DASZZC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098788"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VHE37_01855"
     CDS             complement(<1..1214)
                     /locus_tag="VHE37_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVT34301.1"
                     /translation="MGPTAQKILDLAVKLVYGRRTFTFGVLLALTVVLGWFALHLQPD
                     AGFEKEVPLDHPYMQVFKQYQQAFGGANLVSIALINHQGDIYNEHFLDTLKKVTDAVF
                     FANGVDRSRVQSLFTPGVRYIEIVEGGFSAGDVIPRNYAPSPEMFARIRANVARAGVI
                     GRLVSNDQRGALVVAELLEHDPVTGRKLDYRELAKQLEDIRSKYQDAGISIHIIGFAK
                     IVGDVTDASLDVISFFVIALLLTLLLLWSFCGSIRLSMLPLSAAITAVIWELGLLRLA
                     GFGLDPFAILVPFLILSIGVSHGVQYINGWGNEVTEHRRNSFDASVETFRRLFIPGTV
                     AIVTNVIGFATLAFIKIQIVQEMAINAAMGMLAVIITNKVMLPIWLTWVKLDDADKFR
                     GRQQRRQELGDRL"
     gene            complement(1323..1763)
                     /locus_tag="VHE37_01860"
     CDS             complement(1323..1763)
                     /locus_tag="VHE37_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34302.1"
                     /translation="MNTIAADTPASRRAYRRARQIARERARVRSEQCRVVPQSRRLSE
                     QWLALCVPPLALVLAALAFSSCRLPSPAPLLAALDTPAMHHLGVLFALLAPAISLILQ
                     RSWPMLVLFGVLTLAALTHTDAATACGGGLNQCIEALAWQPQTG"
     gene            complement(1760..2488)
                     /locus_tag="VHE37_01865"
     CDS             complement(1760..2488)
                     /locus_tag="VHE37_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34303.1"
                     /translation="MPRLRNEVEPGVEPADAALAASSLTWLRRMAMFTRDDHPSLYGV
                     RLLFAGVVAIYCATSGPLSGALPLGLPTVLAALAFYAAAVLSLWIGVRSSEALRQQAP
                     RLHVLLDAGILTICVAQDPSPSSPLMMLVLAALIDHGLRFRPQLLGLSAVLGIGILAV
                     NFTSRTLNSPAGMPPPAIWLSFVTSVLMLYFTMFLWAVWVGRHAPSPEHELNQALAEA
                     ARVSAARLREVQRTSAHRTASNSP"
     gene            complement(2567..3292)
                     /locus_tag="VHE37_01870"
     CDS             complement(2567..3292)
                     /locus_tag="VHE37_01870"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVT34304.1"
                     /translation="MTDTLVSYRLDGRVATLTMDDGKANAVSPTMLASLNRALDQAEA
                     DKAVVVLAGREGRFSAGFDLTVLWSGGPAAADMLTGGFELAARMLAFPRPIVAACTGH
                     ALAMGAFLLLACDHRIGADGTYKIGANEVAIGLTMPYAAIELCRQRIGPSYFTRTVID
                     AEIYTPAGAVAAGFLDQVVPATEVMSAAQATAAALAKLNMTAHAATKLRVRESTLRAL
                     RAGIEADAAGFADWARAAPRPAG"
BASE COUNT          625 a         1089 c         1256 g          524 t
ORIGIN      
        1 aggcggtcgc ccagttcctg gcggcgctgc tggcggccgc ggaacttgtc ggcgtcgtcg
       61 agcttcaccc aggtaagcca gattggcagc atgaccttgt tggtgatgat caccgccagc
      121 atgcccatgg cggcgttgat cgccatctcc tgcacgatct ggatcttgat gaaggccagc
      181 gtggcgaagc cgatcacgtt ggtgacgatg gccacggtgc cgggaatgaa caggcgacgg
      241 aaggtttcca ccgaggcatc gaacgagttg cgccggtgct ccgtcacttc gttgccccag
      301 ccgttgatgt actgcacgcc gtggctcacg ccgatggaca ggatcaggaa cggcaccagg
      361 atggcgaagg gatcgaggcc gaagccggcc agccgcagca gtcccagttc ccagatcacc
      421 gcggtgatgg cggcggacag cggcagcatc gacaggcgga tcgagccgca gaagctccac
      481 agcagcagca gcgtgagcag cagcgcgatg acgaagaagc tgatgacgtc gaggctggcg
      541 tcggtgacgt cgccgacaat cttggcgaag ccgatgatgt ggatgctgat gccggcatcc
      601 tggtacttgc tgcggatgtc ttcgagctgt ttggccagtt cgcggtagtc gagcttcctg
      661 ccggtgaccg ggtcgtgctc gagcagttcc gccaccacca gcgcaccgcg ctggtcgttg
      721 gataccagcc ggccgatcac gccggcgcgc gccacgttgg cgcggatgcg cgcgaacatt
      781 tccgggctgg gtgcgtaatt gcgcgggatg acgtcgccgg cggaaaagcc gccttcgacg
      841 atctcgatat agcgcacgcc gggcgtgaac agcgactgca cgcgcgagcg gtcaacgccg
      901 ttggcgaaga acaccgcgtc ggtgactttt ttaagtgtgt cgaggaagtg ctcgttataa
      961 atatcgccct gatggttgat cagcgcgatc gacaccaggt tggcgccgcc gaaagcctgc
     1021 tggtactgct tgaacacctg catgtacggg tgatcgagcg ggacttcctt ctcgaagccg
     1081 gcgtcgggtt gcaggtgcag cgcgaaccag cccagcacca cggtcaatgc cagtagcacg
     1141 ccgaaggtga aggtgcggcg gccatagacc agcttgaccg cgagatcgag aattttttgg
     1201 gcggtcgggc ccaccgtctc ctccgttctt atggccggca attctgcgcg atgtgacagg
     1261 cgcgcgccat ggaccgcgga agaggccggc gggcaatgca gacggcccgc gtatcgtttg
     1321 ggtcaaccgg tctgcggttg ccaggccagc gcttcgatgc actggttcaa gccgccgccg
     1381 caggcggtgg cggcgtcggt atgcgtcagg gccgccagcg tcagcacgcc gaacagcacc
     1441 agcatgggcc aggaccgctg caggatcagg ctgatggccg gagcgagcag ggcgaacagc
     1501 acgccgagat ggtgcatggc cggcgtatcc agcgccgcca gcaagggcgc cggcgacggc
     1561 agccggcagg acgagaaggc aagtgccgcg agcaccagcg caagcggcgg aacgcagagc
     1621 gcgagccatt gttcggacag gcggcgcgac tgcggcacga cgcggcactg ctcgctgcgc
     1681 acgcgcgccc gttcgcgcgc gatctggcgc gcacggcggt aggcgcggcg cgaggcgggc
     1741 gtgtcagcgg cgatcgtatt catggggagt tggacgcggt gcggtgggca gatgttcgct
     1801 gaacttcgcg taggcgtgcg gctgacacac gcgctgcttc tgccagcgcc tggttgagct
     1861 cgtgctcggg cgaaggcgca tggcgcccga cccagaccgc ccacaggaac atggtgaagt
     1921 agagcatcag caccgaggtg acgaaactga gccagatggc cggcggcggc atgccggccg
     1981 ggctgttgag cgtgcgcgag gtgaagttca ccgcgaggat gccgatgccc agcacggcgg
     2041 acaggccgag cagctgcggg cggaaccgca gcccgtggtc gatcagcgcc gccagcacca
     2101 gcatcatcag cggcgaactg ggtgacgggt cctgcgccac gcagatggtg aggattccgg
     2161 catcgagcag cacgtgcagg cgcggcgcct gttggcgcag cgcctcggac gagcggacac
     2221 cgatccacag cgacagcacg gcggcggcat agaacgccag cgcggccagc accgttggca
     2281 gacccagcgg caaggcgccg gagagcgggc ccgaggtggc gcaatagatg gcgacgacgc
     2341 cggcgaacag caggcgtacg ccatagaggg acggatgatc gtcgcgcgtg aacatggcca
     2401 tgcgccgtaa ccaggtaagg gaagaagctg ccagcgccgc gtcggccggt tcgacacctg
     2461 gttcaacctc gttgcgaagt ctcggcatgt ccgctccagg ctcgacgctc gcgggcagtc
     2521 tgatgcgggc tgccgctgga ggcattgact caggtcaacg aggcgctcaa cccgcgggcc
     2581 ggggcgccgc ccgcgcccag tcggcgaatc cggcggcgtc ggcctcgatg ccggcgcgca
     2641 atgccctgag cgtggactcg cgcacgcgca gcttggtcgc ggcgtgcgcg gtcatgttga
     2701 gcttggccag cgcagcagcc gtggcttgcg ccgcgctcat cacctcggtt gccggcacca
     2761 cctggtcgag gaagccggcg gcgacggcgc ccgcaggcgt gtagatctcc gcatcgatca
     2821 ccgtgcgcgt gaagtaagag gggccgatgc gctgccggca cagttcgatg gcggcgtagg
     2881 gcatggtcag cccgatggcg acttcgttcg cgccgatctt gtaggtgccg tcggcgccga
     2941 tgcggtgatc gcaggccagc agcaggaacg cgcccatggc cagcgcatga cccgtgcagg
     3001 cggcgacgat cggccgtgga aacgccagca tgcgcgcggc cagttcgaag ccgccggtaa
     3061 gcatgtccgc cgcggccggg ccgccgctcc acagcacggt gagatcgaag ccggcggaga
     3121 accggccttc gcgtccggcc agtaccacca cggccttgtc cgcttccgcc tggtcgagcg
     3181 cacgattgag agacgccagc atggtcggcg acaccgcatt ggccttgccg tcgtccatgg
     3241 tcagggtggc gacacggcca tcgaggcggt aggaaaccag ggtgtcggtc atgggaactc
     3301 tcagcacgat gcggtggatc gactagaata ttattctaga acgctattcc agagagcgaa
     3361 tgtgagtcaa gccggaaaag cgggacgcgg ccgccgcggc gacatactcg atgccgcgct
     3421 ggcctgcttc aaccgccacg gcatcgaggc ggcggcgatc ggcgacatct gcgcccgcgc
     3481 gggcgccagc gttg
//
LOCUS       DASZZC010000076         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_582297,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000076 DASZZC010000000
VERSION     DASZZC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582297"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /locus_tag="VHE37_01875"
     CDS             <1..1735
                     /locus_tag="VHE37_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVT34305.1"
                     /translation="VEFPIKRVIYRLAERSLLGNLGQVYVGANQMLEARGFHQHPLRV
                     RQTPQSPAAAGAPAPAAPAYPPSATPNPYAPGGPLSSSAPPPPTAYAGDSYAEPAPQL
                     HNPSSTMADAGMPMPQGTWAGADAGGLPGPAAAIAWPSGMNPSRLLDALSQVSAPAAA
                     PAQPVYSDSQLATDMASALDALAQGRQINTWIPPANLALAGQMFDTLYQDHRLHEAFR
                     PLLRRLQYPVMKTALSDPSFFTSPSHPVRNLVHDVFGMVSSLDSPSPQDIKRLTDLIE
                     HLTRQFEVDSERFAIPEARAPSVNETEAEQFLAEQKQRMADHRARTLKMVRRLVAQEL
                     KIRTADRDVPEGLMPLLLSGFGPMLAVNALRGGVSGTPWKESIDLLERMLDSISSSGT
                     SPNERHLAEQADIIINITERLTTIGLDPEKVQRLVGALVDHYDELARNRGRRAVAAQK
                     PAAPPPEKSKEELQEKAVREARQALSAILMVGSWFQVWDATNSQQRWLKLHAYFPTID
                     AVVFDDFMGENHLKLNASNLIRDFIKGLSAPVDPSAAVQRALKLLEPLVDMLPPASEA
                     PVWQPATETMH"
BASE COUNT          292 a          646 c          565 g          250 t
ORIGIN      
        1 agtcgaattc ccgatcaagc gcgtcatcta ccgtctggcg gagcgctcgc tgctgggcaa
       61 cctgggccag gtctacgtcg gcgccaacca gatgctggaa gcacgcggct tccaccagca
      121 cccgctgcgc gtgcggcaga ccccgcaatc gcctgccgcc gccggtgcgc cggcgccggc
      181 ggcgcccgcc tacccgccct ccgcgacacc gaatccgtac gcgccgggcg ggccgctgtc
      241 cagttccgct ccaccaccgc ccaccgctta cgcgggtgac tcgtacgccg agcccgcgcc
      301 gcaattgcac aacccctcgt caacgatggc cgatgccggc atgccgatgc cgcagggcac
      361 ctgggccggt gccgatgccg gcgggctgcc cggcccggcc gcggccatcg cctggcccag
      421 cggcatgaac ccttcgcgcc tgctcgatgc gctgtcgcag gtcagcgcac ccgcggcggc
      481 gccggcgcaa ccggtgtaca gcgattcgca gctggccacg gacatggctt ccgcactcga
      541 cgcgctggcc cagggtcgcc agatcaacac ctggattccg cccgccaacc tcgcgctggc
      601 cgggcagatg ttcgacacgc tgtaccagga ccatcgcctg cacgaggcat tccgcccgct
      661 gctgcgccgc ctgcagtacc cggtgatgaa gaccgcgctg tccgatccct cgttcttcac
      721 cagtccgagc cacccggtgc gcaacctggt gcatgacgtg ttcggcatgg tgtcgagcct
      781 cgacagcccg tcgccgcagg acatcaagcg gctcaccgac ctgattgagc acctgacgcg
      841 ccagttcgag gtcgattcgg aacgcttcgc catcccggag gcgcgcgcgc cgtcggtcaa
      901 cgagaccgaa gccgagcagt tcctggccga gcagaagcag cgcatggccg atcaccgggc
      961 gcgtacgctc aagatggtgc ggcgcctggt ggcgcaggaa ctgaagatcc gcaccgccga
     1021 ccgcgacgtg cccgaaggac tgatgccgct gctgctgtcc ggtttcggcc cgatgctggc
     1081 ggtgaatgcg ctgcgcggcg gcgtgtccgg cacaccgtgg aaggaatcga tcgacctgct
     1141 cgagcgcatg ctcgacagca tttcctccag cggcacctcg cccaacgagc ggcacctggc
     1201 cgagcaggcc gacatcatca tcaacatcac cgagcggctc accaccatcg ggctcgaccc
     1261 ggagaaggtg cagcggctgg tcggcgcgct ggtcgatcat tacgacgagc tggcgcgcaa
     1321 ccgcggccgg cgtgccgtcg cggcacagaa gccggccgcg ccgccgccgg agaagtcgaa
     1381 ggaagaactg caggaaaaag ccgtgcgcga ggcgcgccag gcgctgtccg ccatcctcat
     1441 ggtcggcagc tggttccagg tctgggacgc caccaacagc cagcagcgct ggctcaagct
     1501 gcacgcctat ttcccgacca tcgacgcggt ggtgttcgac gacttcatgg gcgagaacca
     1561 cctgaagctc aacgcctcca acctgatccg cgatttcatc aagggcctgt cggcgccggt
     1621 cgatcccagc gcggcggtac agcgcgcgct caagctgctg gagccgctgg tggacatgct
     1681 gccgccggcc agtgaagcgc cagtgtggca gccggccacc gaaacgatgc actgagcggc
     1741 gcgctcagtc gtc
//
LOCUS       DASZZC010000077         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_646630,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000077 DASZZC010000000
VERSION     DASZZC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646630"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VHE37_01880"
     CDS             complement(<1..413)
                     /locus_tag="VHE37_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT34306.1"
                     /translation="MMQLACIAEGLYLRHAAAMLHSVLSLTPAPAKVHVLHESEVPAE
                     DRTRFESVVHKFSGQVVWHRMPPEIADDFPGGYFHRSIWVRVLLPRLLPDVERVLYLD
                     ADLIAADSLLPLWQTPLDDHLLAAVTNPLYPFMLP"
     gene            complement(410..1399)
                     /locus_tag="VHE37_01885"
     CDS             complement(410..1399)
                     /locus_tag="VHE37_01885"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HVT34307.1"
                     /translation="MSAAEHALHLACACDAGYAPHAAAMLDSVLSHGDPARWTLHLLH
                     APDLPANICEQLAAPVRARGARLQFHQVDDQRVAALQRMERIGSVMWYRMFLPELLPE
                     VARVLYVDCDILAATDLVPLLTTDLQGLPLGAVRNVFDPPSRHRPQQLGLTSARDYFN
                     SGVLLMDLAAWRREGWAQRVLDIARQRHSELLWPDQDALNLAFAGRWLPLHPRWNAMN
                     SLYYFDDLGGAYSEAERAEAISSPGLLHFEGPGFCKPWNALCKHPYRAAYFASRARTA
                     WPQVQLQDDTLRTRLLRLLPMRQIPRALALVERIERGVARRWRRLTGQSRAAA"
     gene            complement(1396..2592)
                     /locus_tag="VHE37_01890"
     CDS             complement(1396..2592)
                     /locus_tag="VHE37_01890"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT34308.1"
                     /translation="MPDTLHSAAPVTPASAAAARLQGLDGLRGLAMLMVIACHLNVLW
                     IGWTGLASFFVLSGFLITRILLSDRAQAESLGGYFRRFYIRRTLRVFPIYYIYLLLLT
                     VAALTVPALTAVRGELPTAFLYLYNFHMMFPHEHTRTLGHLWSLSVEEQFYLVWPWVV
                     AFLSRSALRNLCMALIVIGPLLRWWTANVLLPAWGLEGPPQSLIYMYIFTGTHLDGFA
                     FGALLNLVDVRARDWQVGALLLVSLALGLLLNQGMDISPSALGWPLFLPAAYQYTWGY
                     SVVDLFWALTIAAIVGGSGAVKKLYTLPLLDYLGKRSYSTYIVHFPLLALMNPLWQKS
                     MNLLGDLPGTAAFAVPYLAIVFAVSGLSFRYVETPISELKDRFSTSRRKAAAASDGAG
                     ARAEAS"
     gene            2660..3019
                     /locus_tag="VHE37_01895"
     CDS             2660..3019
                     /locus_tag="VHE37_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34309.1"
                     /translation="MTARTLSKAGAAALAALLVIGLALRLAAPGWVLRQVNQRMAEMG
                     DYHGHVEDVTLHLWRGAYELHHLKIDKASGKVPVSLLDAPLIDLSVSWRALLHGRVVA
                     TVQFDRPVVSLVDGSGQ"
     gene            3056..3721
                     /locus_tag="VHE37_01900"
     CDS             3056..3721
                     /locus_tag="VHE37_01900"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HVT34310.1"
                     /translation="MLENLLPIRLDEVQVADGEVTFHNFISNPPVDLHATGVQAVVTN
                     LTNVRGQGRRPAEFKARAAILGEAPMETSARFDPFGHFDDFAFEVKVTRIELPRLNDL
                     LRAYALLDVKAGEGEFVMQLDAAHGRVSGYAKPLFHDIEVLNPKEDVKNPLKFAWKTV
                     AATLINIFKNHKHDQFGTRIPIEGPIDQPKTSALAAIGGILHNAFVQAFKPEFEDLKK
                     DAK"
     gene            3761..>4201
                     /locus_tag="VHE37_01905"
     CDS             3761..>4201
                     /locus_tag="VHE37_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HVT34311.1"
                     /translation="MSRKTASIHAVILAAGQGTRMKSALPKVLHTVGGRPMLAHVLDA
                     ARAAGATQLHVVHGHGADAVRAAFAGAADLNWVMQAQQLGTAHAVLQAMPHIPDDALV
                     LVLSGDVPLTSADTLRQLAGAGASGLALCTVALAQPHGYGRIVRD"
BASE COUNT          753 a         1379 c         1480 g          589 t
ORIGIN      
        1 tatggcagca tgaacggata cagcggattg gtcaccgccg ccagcagatg atcgtccagc
       61 ggcgtctgcc acagcggcag caggctgtcg gcggcgatca ggtcggcgtc gaggtacagc
      121 acgcgctcga catccggcag caggcgcggc agcagcacgc ggacccagat cgagcggtgg
      181 aaatagccgc cgggaaaatc gtcggcgatc tccggcggca tgcgatgcca caccacctgg
      241 ccgctgaatt tgtgcaccac cgactcgaag cgcgtgcgat cttccgcggg cacctcgctc
      301 tcgtgcagca cgtgcacctt ggcgggtgcg ggcgtcagcg acagcaccga gtgcagcatg
      361 gccgcggcgt ggcgcaggta gaggccctcg gcaatgcagg ccagctgcat catgcggcgg
      421 cgcgactctg gcccgtgagc cggcgccagc gccgtgccac gccgcgctcg atccgctcga
      481 ccagcgccag cgcacgcgga atctggcgca tcggcagcag ccgcagcagg cgcgtgcgca
      541 gcgtgtcgtc ctgtagctgc acctgcggcc aggcggtgcg cgcgcgcgaa gcgaaatacg
      601 ccgcgcgata gggatgcttg cacagcgcgt tccacggctt gcagaagccg ggaccctcga
      661 aatgcagcag gcccggtgac gaaatggcct cggcgcgctc ggcttcgctg tacgcgccgc
      721 cgagatcgtc gaagtagtac aagctgttca tcgcgttcca gcgcgggtgt agcggcagcc
      781 agcggccggc gaaggcgagg ttgagtgcgt cctggtccgg ccacagcagc tcgctgtgcc
      841 gttgccgtgc gatgtccagc acgcgctgcg cccagccctc gcggcgccag gcagcgaggt
      901 ccatcagcag cacgccgctg ttgaagtagt cgcgcgcaga cgtcagcccg agctgctgcg
      961 gacggtgccg cgatggtgga tcgaacacgt tgcgcaccgc gcccagcggc agaccctgca
     1021 gatcggtggt gagcagcggc acgagatcgg tggcggccag gatgtcgcaa tcgacgtaca
     1081 gcacgcgcgc aacctcgggc agcaactcgg gcaggaacat gcggtaccac atgacgctgc
     1141 cgatacgctc catgcgctgc agggcagcca cgcgctggtc gtcgacttgg tggaattgca
     1201 ggcgcgcgcc gcgcgcgcgt acgggcgcgg cgagctgttc acagatgttc gccggcaggt
     1261 cgggcgcgtg cagcagatgc agcgtccagc gcgccggatc gccgtgcgac agcaccgagt
     1321 cgagcatggc cgcggcatgc ggcgcgtagc cggcatcgca ggcgcaggcc aggtgcaatg
     1381 cgtgctccgc tgcgctcatg aagcttcggc gcgcgcgccc gcgccgtcgg atgcggcagc
     1441 ggccttgcgc cgcgacgtgc tgaagcgatc cttcagttcg ctgatcggcg tctcgacgta
     1501 gcggaacgac aggcccgaca cggcgaagac gatggcgaga tacggcacgg cgaaagcggc
     1561 ggtgcccggc agatcgccga gcaggttcat cgacttctgc cacagcgggt tcatcagagc
     1621 cagcagcggg aagtgcacga tgtaggtcga atacgaacgc ttgccgaggt aatcgagcag
     1681 cggcagcgtg tacagcttct tcaccgcacc gctgcccccg acgatggccg cgatggtgag
     1741 ggcccagaac aggtcgacca cgctgtagcc ccaggtgtac tggtatgccg ccggcaggaa
     1801 cagcggccag cccagcgcgc tgggcgagat gtccatgccc tggttgagca gcaggcccag
     1861 cgccagcgag accagcagca gcgcgcccac ctgccagtcg cgcgcgcgca cgtcgaccag
     1921 gttgagcagc gcgccgaagg cgaaaccgtc gagatgcgtg ccggtgaaga tgtacatgta
     1981 gatcaggctc tgcggcgggc cttccaggcc ccaggccggc agcagcacgt tggccgtcca
     2041 ccagcgcagc agcgggccga tgacgatcag cgccatgcag agattgcgca gcgcgctgcg
     2101 cgacaggaag gccaccaccc acggccacac caggtagaac tgctcctcca ccgacagcga
     2161 ccacaggtgt ccgagcgtgc gcgtgtgctc gtgcggaaac atcatgtgga agttgtacag
     2221 atagaggaac gccgtcggca gttcgccgcg caccgccgtc aatgccggca ccgtcagcgc
     2281 cgccacggtg agcagcagca ggtagatgta atagatgggg aacacgcgca gcgtgcggcg
     2341 gatgtagaag cggcggaagt agccgccgag cgattcggcc tgcgcgcggt ctgacagcag
     2401 gatgcgggtg atgagaaagc cggacagcac gaagaagctg gccagcccgg tccagccgat
     2461 ccacagcacg ttgagatggc aggcgatgac catcagcatg gccagcccgc gcaggccgtc
     2521 gaggccttgc aggcgggccg ccgcggccga ggcaggtgtt accggcgcag cggaatggag
     2581 ggtgtcgggc accggtggat gatatcgccg attgcccggc acccgaacca ttggcgttgc
     2641 ggcgctgcca aaaagcagca tgaccgcacg gactctgagc aaggcaggcg cggccgccct
     2701 ggcggccttg ttggtcatcg gcctcgccct gcgcctggcc gcacccggct gggtactgcg
     2761 ccaggtcaac cagcgcatgg ccgagatggg cgattaccac ggccatgttg aagacgtcac
     2821 cctgcacctg tggcgcggcg cgtacgaact ccatcacctg aagatcgaca aggcctcggg
     2881 caaggtaccg gtgtcgctgc tcgatgcgcc gctgatcgat ctgtcggtga gctggcgcgc
     2941 gctgctgcac ggccgggtgg tggcgacggt gcagttcgat cgcccggtgg tgtcgctggt
     3001 cgacggctcg ggccagtagg acagccaggc cggcgccggc gtcgactggc gccagatgct
     3061 ggagaacctg ctgccgatcc ggctcgacga ggtgcaggtg gccgacggcg aggtcacctt
     3121 ccacaacttc atatccaatc cgccggtgga cctgcatgcc accggcgtgc aggcggtggt
     3181 gaccaacctg accaatgtgc gcggccaggg ccggcggccg gcggagttca aggcgcgcgc
     3241 cgccatcctg ggcgaggcgc cgatggagac cagcgcgcgc ttcgatccgt tcggccactt
     3301 cgacgacttt gcctttgagg taaaagtgac gcgtatcgaa ctgccacggc tcaacgacct
     3361 gctgcgcgcc tacgcgctgc tcgacgtgaa agccggcgag ggcgaattcg tcatgcagct
     3421 cgacgccgcg cacggccgcg tcagcggcta cgccaagccg ctgttccatg acatcgaagt
     3481 gctgaatccg aaggaagacg tgaagaaccc gttgaagttt gcgtggaaga cggtggccgc
     3541 cacgctgatc aacattttca agaaccacaa gcatgaccag ttcggcacgc gcatccccat
     3601 cgaggggccg atcgaccagc cgaaaacctc ggcgctggcg gccatcggcg gcatcctgca
     3661 caacgctttc gtgcaggcgt tcaagcccga gttcgaggac ctgaagaagg atgcaaagta
     3721 gcggcgccgc gggcgggcgg ggacatgcga aactaaacgc atgagccgca aaaccgcttc
     3781 catccacgcc gtgatcctcg ccgccggcca gggcacgcgc atgaaatccg cgctgcccaa
     3841 ggtgctgcac acggtcggcg gccggccgat gctggcccat gtgctggatg ccgcgcgcgc
     3901 tgccggcgcc acgcagctgc acgtggtgca cggacatggc gcggacgcag tgcgcgccgc
     3961 ctttgcgggt gccgcggacc tgaactgggt gatgcaggcg cagcagctgg gcaccgcgca
     4021 cgcggtgctg caggccatgc cgcatattcc cgatgacgcg ctggtgctgg tgctgtccgg
     4081 cgacgtgccg ctgacctcgg ccgatacgct gcgccagctg gcgggcgccg gcgcgagcgg
     4141 gctggcgctg tgcacggtcg cactggcgca gccgcacggc tacggccgca tcgtgcgcga
     4201 c
//
LOCUS       DASZZC010000078         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_456379,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000078 DASZZC010000000
VERSION     DASZZC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456379"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..2137
                     /locus_tag="VHE37_01910"
     CDS             149..2137
                     /locus_tag="VHE37_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVT34312.1"
                     /translation="MSLDLSRFHQTFFEESREGLDTMESALLKLDIGRADSEVINTIF
                     RAAHSIKGGAATFGFSSVAEYTHGVETLLDQMRSGKRAVTQPDVDLLLRAVDVLRGLL
                     AAASDGSPLDTQAIAASQAELKATLSGGAASAAAPAAAAATSAAGGRWHIRFAPHADL
                     FASGNDPLLILRELSALGRMQAEIDERHLTKLREAQADECLLAWNITLEGGTRAEIDN
                     VFAWVEGECELAVEAAPAQAAATAAAPPVAAAAAAPAKAVLSVIEGGKAEHKASESSI
                     RVGTEKIDALINLVGELVITQAMLTQQASALDPVAHEKLLNGLSQLDRNTRMLQEAVM
                     STRMLPMDFVFSRFPRMARDLAAKLGKQVRLLTVGEATELDKGVIERIADPLTHLVRN
                     SLDHGLEKADDRAAAGKDPCGTIKLSAAHQGGHIVIEISDDGRGLDRERILRKAREKN
                     LPCSESMSDADVHQLIFAPGFSTAEVVTDVSGRGVGMDVVKKNIQALSGQVELSSRPG
                     HGTTVTIRLPLTLAILDGMSVRVGEEIFIVPLNCVVESLQPAAGQVKSVAQSGQVVRV
                     RNEYLPLVALHQVFNLPTQVRDARDGILVLLESEGKKIAALVDELVGQQQVVIKSLEA
                     NYRRVHGISGATILGDGRVALILDVADLVRGSSRAMAA"
     gene            2159..2692
                     /locus_tag="VHE37_01915"
     CDS             2159..2692
                     /locus_tag="VHE37_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVT34313.1"
                     /translation="MNLENTETRSSSRAGAAKPGDAAGAQEFLTFTLGAEEYGVDILK
                     VQEIRGYDTVTRIPDAPPYIKGVINLRGTIVPVIDLRIKLKLDKAEYNSFTVMIILNV
                     QRRVVGIVVDGVSDVMQLAAEQIRPPPEFGGSLDTRFISGIGTFDSRMLILVDIEKLL
                     RSQDLALLPAAGDAATH"
     gene            2712..>4919
                     /locus_tag="VHE37_01920"
     CDS             2712..>4919
                     /locus_tag="VHE37_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVT34314.1"
                     /translation="MKKSVKKSAPRASAKKAPVKAGGQAANDRRIRAGVEKIATNLMV
                     ADVDLTITYANPSVLAMLRAAQSDIRRDLPHFDADRVLGANIDIFHKHPEHQRRMLST
                     LDHAHNARIKVGGRTFALNLSPIRDAAGKPDGFIVEWKDMTEELKTLEAEKSKAAGFE
                     SQLAAISKAQAVIEFTLDGKIVHANENFLGAMGYSMDEIRGQHHGMFVDPAYRSSADY
                     RAFWEKLGRGEYDAGQYKRIAKGGREIWIQASYNPILDGSGKPCKVVKYATDITEQKM
                     KSADYEGQLSAISKAQAVIEFTLEGKIIQANENFLNAMGYRAEEIRGQHHSMFVDPAF
                     RSSTEYRLFWEKLGRGEYDSGQYKRVAKGGREIWIQASYNPILDLNGKPFKVVKYATD
                     VTEQVKAQQVMQQAVAQTQEVVGATKDGDLTRRIPMEGKTGSIEQLCGGVNSLVDSFA
                     SIVGRIKVAVDTISTAAKEIASGNTDLSARTEQQAASLEETASSMEELTSTVKQNAEN
                     AKQANQLAIGAGDVAQKGGKVVSEVVTTMAAINESSKKIVDIISVIDGIAFQTNILAL
                     NAAVEAARAGEQGRGFAVVAAEVRSLAQRSAGAAKEIKSLIGDSVEKVGNGAKLVEQA
                     GKTMDEIVGSVKRVTDIMAEISAASQEQSSGIEQVNQTITQMDEVTQQNAALVEEASA
                     SARSLEEQSIGLTHSVSQFKLDESAIRALPAAPASAPVAAARPAAPAARTPARP"
BASE COUNT          949 a         1622 c         1640 g          708 t
ORIGIN      
        1 tccgcctcgt acttccccgc acggcgcaat tgcgccgacc aacggtcggg aatgcgcctt
       61 gcctctgctc aaggcccgcg cagctcgtgc cgctaagccg acacctgcct gcgcgcacgc
      121 ggttgctgtg ccacaccgat cagcctgaat gagcctcgac ctgtcccgtt ttcaccagac
      181 cttcttcgaa gagagccgcg aaggtctgga caccatggag tcggcgctgc tcaagctcga
      241 catcgggaga gctgattcag aagtcatcaa caccattttc cgcgcggcgc actcgatcaa
      301 gggcggcgcc gccaccttcg gcttctccag cgtggcggaa tacacgcacg gcgtggagac
      361 gctgctcgac cagatgcgct cgggcaagcg cgcggtgacc cagcccgatg tcgacctgct
      421 gctgcgcgcg gtcgacgtgc tgcgcggcct gctggccgcg gccagcgacg gctcgccgct
      481 ggacacacag gccatcgccg cgtcgcaggc cgaactcaag gccacgcttt ccgggggtgc
      541 tgcgtccgct gcggcgccgg cggcagcggc cgccacgtcc gcggcgggcg ggcgctggca
      601 catccgcttt gcgccgcacg cggacctttt tgcctccggc aacgacccgc tgctgatcct
      661 gcgcgaactc tccgccctgg gccgcatgca ggccgagatc gacgagcgcc atctcaccaa
      721 gctgcgcgag gcgcaggccg acgaatgcct gctggcctgg aacatcacgc tcgaaggggg
      781 cacgcgagcc gagatcgaca acgtgttcgc ctgggtggaa ggcgagtgcg aactggcggt
      841 ggaagccgcg ccggcgcagg ccgcagcgac ggcggcggct ccgccggttg ccgccgcggc
      901 cgcggcgccg gccaaggccg tgctcagcgt gatcgaaggc ggcaaggccg agcacaaggc
      961 gagcgaaagc tcgatccgcg tcggcaccga gaagatcgac gcgctgatca acctggtcgg
     1021 cgaactggtc atcacccagg ccatgctgac gcagcaggcc agcgcgctcg acccggtggc
     1081 gcacgagaaa ctgctcaacg ggctgtcgca gctcgaccgc aacacgcgca tgctgcagga
     1141 agcggtgatg tccacgcgca tgctgccgat ggatttcgtc ttcagccgct ttccgcgcat
     1201 ggcgcgcgat ctcgccgcca agctgggcaa gcaggtgcgg ctgctcaccg tgggcgaagc
     1261 caccgagctg gacaagggcg tgatcgagcg catcgccgat ccgctcacgc acctggtgcg
     1321 caactcgctg gatcacggcc tggagaaagc cgatgaccgc gccgccgccg gcaaggatcc
     1381 ctgcggcacg atcaagctgt cggccgcgca ccagggcggg catatcgtca tcgagatttc
     1441 cgacgacggc cgcgggctgg accgcgaacg catcctgcgc aaggcgcgcg aaaagaacct
     1501 gccgtgctca gagtcgatga gcgatgccga cgtgcaccag ctgatcttcg ccccgggctt
     1561 ttccacggcc gaggtggtga cagatgtttc gggccgcggc gtgggcatgg acgtggtgaa
     1621 gaagaacatc caggcgctgt cgggccaggt ggagctgtcc agccggccgg gccacggcac
     1681 caccgtgacc atccgcctgc cgctgacgct ggccatcctc gacggcatga gcgtgcgcgt
     1741 gggcgaagag atcttcatcg tgccgctcaa ctgtgtggtc gagtcgctgc agccggcggc
     1801 cgggcaggtg aagtcggtgg cgcagtccgg ccaggtggtg cgcgtgcgca acgagtacct
     1861 gccgctggtg gcgctgcacc aggtgttcaa cctgcccacg caggtgcgcg acgcgcgcga
     1921 cggcatcctg gtgctgctcg aatcggaagg gaaaaaaatc gctgcgctgg tcgacgaact
     1981 ggtcggccag cagcaggtgg tgatcaagtc gctggaagcc aactaccgcc gcgtccatgg
     2041 catctcgggc gcaaccatcc tgggggatgg gcgggtggcg ctgatcctcg acgtggccga
     2101 cctggtgcgg ggcagcagcc gcgccatggc cgcatgaaga cgaatcaatg gaggaaacat
     2161 gaatctggaa aacacggaaa cgcgcagctc atcgcgtgcc ggcgcggcca agccgggtga
     2221 cgcggcgggc gcgcaggaat tcctcacctt caccctcggc gccgaggaat acggcgtcga
     2281 catcctgaag gtgcaggaga tccgcggtta cgacacggtg acgcgcatcc ccgatgcgcc
     2341 gccgtacatc aagggcgtga tcaacctgcg cggcaccatc gtcccggtga tcgacctgcg
     2401 catcaagctc aagctggaca aggccgagta caacagcttc acggtgatga tcatcctcaa
     2461 cgtgcagcgg cgcgtggtcg gcatcgtggt cgacggcgtg tccgacgtaa tgcagctggc
     2521 cgccgagcag atccgcccgc cgccggaatt cggcggctcg ctggacaccc gtttcatctc
     2581 cggcatcggc acgttcgaca gccgcatgct gatcctggtc gacatcgaaa agctgctgcg
     2641 ctcgcaggac ctggccttgc tgccggctgc gggcgacgcg gctacccact aacgcaacac
     2701 cgaggaatcc catgaagaaa tcagtgaaga agtccgcgcc gagggcgtcg gccaagaagg
     2761 caccggtgaa ggcgggtggc caggctgcca acgaccgccg catccgcgcc ggcgttgaga
     2821 agatcgccac caacctgatg gtggccgacg tcgatctcac catcacctat gccaacccgt
     2881 cggtgctggc catgctgcgt gcggcgcaat ccgatatccg ccgcgacctg ccgcatttcg
     2941 acgccgaccg cgtgctcggt gccaacatcg acattttcca caagcatccc gagcaccagc
     3001 gccgcatgct cagcacgctc gaccatgcgc acaacgcgcg catcaaggtg ggcggccgca
     3061 ccttcgccct caacctgagc ccaatccgcg atgccgccgg caagccggat ggcttcattg
     3121 tcgaatggaa ggacatgacc gaggagctga agacgctgga ggcggagaag tccaaggcgg
     3181 caggcttcga gtcgcaactg gccgccattt ccaaggcaca ggcggtgata gagttcaccc
     3241 tcgacggcaa gatcgtccac gccaacgaga atttcctcgg tgccatgggc tacagcatgg
     3301 acgagatccg cggccagcat cacggcatgt tcgttgatcc cgcgtaccgc agcagcgcgg
     3361 actatcgcgc gttctgggaa aagctcggcc gcggcgaata cgacgccggc cagtacaagc
     3421 gcattgccaa gggtggccgc gaaatctgga tccaggcgtc ctacaacccg atcctcgacg
     3481 gcagcggcaa gccgtgcaag gtggtcaagt acgccaccga catcaccgag cagaagatga
     3541 agtcggcgga ttacgagggc cagctgtcgg ccatttccaa ggcgcaggcg gtgatcgagt
     3601 tcaccctgga gggcaagatc atccaggcca acgaaaactt cctcaatgcc atgggctacc
     3661 gcgccgagga aatccgcggg caacaccatt ccatgttcgt cgacccggca ttccgcagca
     3721 gcaccgagta ccgcctgttc tgggaaaagc tcgggcgcgg cgaatacgac agcggccagt
     3781 acaagcgggt tgccaagggt ggccgtgaaa tctggatcca ggcgtcgtac aacccgatcc
     3841 tcgatctcaa cggcaaaccg ttcaaggtgg tcaagtacgc caccgacgtg accgagcagg
     3901 tcaaggcgca gcaggtcatg cagcaggcgg tggcgcagac gcaggaagtg gtcggcgcga
     3961 ccaaggacgg cgacctcacg cggcgcatcc cgatggaggg caagaccggc tcgatcgagc
     4021 agttgtgcgg cggcgtgaac tccctggtcg attcgttcgc ctcgatcgtg gggcgcatca
     4081 aggtggcggt cgacaccatt tccaccgcgg ccaaggagat cgccagcggc aacaccgacc
     4141 tgtcggcccg caccgagcag caggcggcct cgctggaaga aaccgcttcg tcgatggaag
     4201 agctgacctc caccgtcaag cagaacgcgg agaacgccaa gcaggccaac cagcttgcca
     4261 tcggcgccgg cgacgtggcg cagaagggcg gcaaggtggt gagcgaagtg gtcaccacca
     4321 tggcggcgat caacgagtcg agcaagaaga tcgtcgacat catcagcgtg atcgacggca
     4381 tcgccttcca gaccaacatc ctggcgctga atgccgcggt ggaagccgcg cgcgccggcg
     4441 agcagggccg cggcttcgcg gtggtggcgg ccgaggtgcg gtcgctggcc cagcgcagtg
     4501 ccggtgccgc caaggagatc aagagcctga tcggcgattc ggtggagaag gtcggcaacg
     4561 gcgccaagct ggtcgagcag gccggcaaga ccatggacga gatcgtcggc agcgtgaagc
     4621 gcgtgaccga catcatggcc gagatcagcg ccgcgtcgca ggagcagtcc agcggcatcg
     4681 agcaggtgaa ccagaccatc acgcagatgg acgaagtcac ccagcagaac gccgcgctgg
     4741 tggaagaagc cagtgcctcg gcccgttcgc tggaagaaca gtcgatcggc ctcacgcact
     4801 cggtcagcca gttcaagctg gatgagtcgg ccatccgtgc actgcctgcc gcgccggcga
     4861 gcgcgccggt tgcggcggcc aggcctgctg cgccggcggc ccgtacgccg gcacgtccg
//
LOCUS       DASZZC010000079         3643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_906027,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000079 DASZZC010000000
VERSION     DASZZC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906027"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..3319
                     /locus_tag="VHE37_01925"
     CDS             191..3319
                     /locus_tag="VHE37_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT34315.1"
                     /translation="MPTTTQRAAVPAPGRSRLGWTQSLQFKLGALFIALTLVLGAGTL
                     LAGKLLVNDELVAETFRYELESGQRLSLQFSEVARRVELLAAVMAQLAADPHLPVPDL
                     IAHDANSALVVAAGIWPEPRTLDPARDRASRYWVRDAGGALVAHDDYNDPRTVPYYHE
                     KWYMPAHHAQPNRCYWTPAYHEPLSKRDMVSCAMPILGAGGTFEGVVTVSLGLADFNQ
                     QFEAATRKDLGYSLLVDFEDRLLAMSALANEKLGAAGPRPRNLPELARNLPAFNPVAV
                     AMRQQDEQFVQAAKGAPSYREPDIDVLKQDTRDLSRDEAAEALSQLWAAQAGFQAAPT
                     RQQIAHDAILDEPAFASSVPLPGTPWRLLRITPATEGFAGASYIFLRTLMVIGGGVLL
                     ALLVVFIALRLMVIGPLRDMTARLASAESTDDAMKVSLDESPANEIGLLAYWLNQRVR
                     QLRELMQRTVTTNAQLVSASDERRKTQEALARVTERSALALQSVSDGVIATDESGLVE
                     DMNPVAESLTGFPLRQARSKDFADIFIARLGETGTPLPNLAQVAVQRGSKLEYLEGVT
                     LFGAGGMHRDVRLTVTPIRTRHNRVTGAVVVFRERDRGGGAEAQALAHDRSLRDPLTG
                     LPMRLLCDSRIRALIEASRLEPRVHALLLVDIDRLREINDIGGPAAGDQVLTRVAEVL
                     VSSAGKAGEVFRLRGGQFAVVLENLDSARARVFAETLREFVAGAHYAWDGKDFAVSAS
                     IGVTEFDERDDDPVPVIARAGEAAAAAKRAGRNQVMLHDAGMSNARADDAAWVKRIRS
                     GLEQNLFHFTTQALQPSAALAGEGQPYEVLLALEDEEGFWTAPANFLPPAQRHQLDGE
                     IDRWVLSRVISHLAHDEAAVERLGYVGINLAPSTISDSHFLEFLAQQLQQLPNLPPRK
                     LCFELRESDVIDHAREAQIFCAAMRSIGCRVAIDHFSGRNSSTLALIRGLGAQLAKID
                     GLAYAAIASDPLQQQLAESVIKLARALHLRVAVCNLENGPQLEAWRRLGADYYQGHAI
                     AKPSPLVFSPLR"
     gene            complement(3316..>3643)
                     /locus_tag="VHE37_01930"
     CDS             complement(3316..>3643)
                     /locus_tag="VHE37_01930"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="5'-3' single-stranded-DNA-specific exonuclease;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HVT34316.1"
                     /translation="LDSDGALADAELNLDTARLLEQAGPWGQGFAAPLFENEFDIVEA
                     RAVGQQQDHARYRLRSGSFSGAAIHFHAPAVVPAGRVRCLYRLNVNRWNGTESADLLI
                     ELIEPL"
BASE COUNT          580 a         1280 c         1238 g          545 t
ORIGIN      
        1 cacctgctcc tgtctggtta ttatttatat aatatattta tctataatat attttttata
       61 acatcgtcag gacgggtgcg gtgatgtcgg aaaattgccc acaggctgtc cctccatctg
      121 ctccgggcgg atgccggcag cgtgtgcgat gatcctgaac cgcacgtcgt aaccgaatcc
      181 cgacaactcg atgccgacta ccacccagcg tgccgccgtc ccggcacctg gccgcagccg
      241 actcggctgg acccaaagcc tgcagttcaa gctcggcgcg ctgttcatcg cgctcacgct
      301 ggtgctcggc gccgggacgc tgctggccgg caagctgctg gtcaacgacg agttggtggc
      361 cgagaccttc cgctacgaac tggaaagcgg ccagcgcctg tcgctgcagt tctccgaggt
      421 ggcacggcgg gtggagctgc tggcggcggt gatggcccag ctggccgccg atccgcatct
      481 gccggtgccg gacctgatcg cccacgacgc caacagcgcg ctggtggtgg ccgccggcat
      541 ctggccggaa ccgcgcacgc tcgaccccgc gcgcgaccgc gccagccgct actgggtgcg
      601 cgatgccggt ggcgcgctgg tggcgcacga cgactacaac gatccgcgca ccgtgcccta
      661 ctaccacgaa aagtggtaca tgcccgcgca ccacgcgcag cccaaccgct gctactggac
      721 gcccgcgtat cacgagccgc tgtccaagcg cgacatggtc agctgcgcca tgcccatcct
      781 cggtgccggt ggcaccttcg aaggcgtggt gaccgtgagc ctcggcctgg ccgatttcaa
      841 ccagcagttc gaagccgcca cgcgcaagga cctgggctac agcctgctgg tggatttcga
      901 agaccgcctg ctggccatgt cggcgctcgc caacgagaag ctcggcgccg ccggcccgcg
      961 cccgcgcaac ctgccggagc tggcgcgcaa cctgccggca ttcaacccgg tggcggtggc
     1021 catgcgccag caggacgagc agttcgtgca ggccgccaag ggcgcgccga gctaccgcga
     1081 acccgacatc gatgtactga agcaggacac gcgcgacctg tcgcgcgacg aggcggccga
     1141 agcgctgtca cagctgtggg cggcgcaggc cggcttccag gcggcgccga cgcgccagca
     1201 gatcgcgcat gacgccattc tcgacgagcc ggctttcgcc agcagcgtgc ccttgccggg
     1261 cacgccgtgg cgcctgctgc gcatcacgcc ggccaccgag ggctttgccg gcgccagcta
     1321 catctttctg cgcacgctga tggtgatcgg cggcggcgtg ctgctggcgc tgctggtggt
     1381 gttcatcgcg ctgcgcctga tggtgatcgg cccgctgcgc gacatgaccg cgcggctggc
     1441 gtcggccgag agcaccgacg acgccatgaa ggtctcgctg gacgaatcgc cggccaacga
     1501 gatcggcctg ctggcctact ggctgaacca gcgcgtgcgc cagctgcgtg agctgatgca
     1561 gcgcaccgtg accaccaacg cacagctggt gtcggcctcc gacgagcggc gcaagacgca
     1621 ggaagcgctg gcccgcgtca ccgagcgcag cgcgctggcc ctgcagtcgg tgtccgacgg
     1681 cgtcatcgcc accgacgaat ccggactggt ggaggacatg aacccggtgg cggaatcgct
     1741 caccggcttc ccgctgcgcc aggcgcgcag caaggacttc gccgacatct tcatcgcgcg
     1801 cctgggcgag accggcacgc cgctgcccaa cctggcgcag gtggcggtgc agcgcggcag
     1861 caagctcgaa tacctggaag gcgtgacgct gttcggcgcc ggcggcatgc accgcgatgt
     1921 gcgcctgacg gtgacgccga tccgcacgcg ccacaaccgc gtcaccggcg cggtggtggt
     1981 gttccgcgag cgcgaccgcg gcggcggcgc cgaggcgcag gcactggcgc acgaccgcag
     2041 cctgcgtgat ccgctcaccg gcctgcccat gcgcctgctg tgcgacagcc gcatccgcgc
     2101 gctgatcgag gcctcgcgcc tggagccgcg cgtgcacgcg ctgctgctgg tcgacatcga
     2161 ccgcctgcgt gagatcaacg acatcggcgg accggcggca ggcgaccagg tgctgacgcg
     2221 cgtggccgag gtgctggtga gcagcgccgg caaggccggc gaagtgttcc gcctgcgcgg
     2281 cggccagttc gcggtggtgc tggaaaacct cgacagcgcg cgcgccaggg tgttcgccga
     2341 gacgctgcgc gaattcgtcg ccggcgccca ctacgcgtgg gacggcaagg actttgcggt
     2401 cagcgccagc atcggcgtca ccgagttcga cgaacgcgac gacgacccgg tgccggtgat
     2461 cgcgcgcgcc ggcgaagcgg cggcagcggc caagcgggcc ggccgcaacc aggtgatgct
     2521 gcatgacgcc ggcatgagca acgcccgcgc cgacgacgcc gcctgggtca agcgcatccg
     2581 cagcggcctg gagcagaacc tgttccactt caccacgcag gcgctgcagc cgtcagccgc
     2641 actggccggc gagggccagc catacgaagt gctgctggcg ctggaagacg aggaaggttt
     2701 ctggaccgcg ccggccaact tcctgccgcc ggcgcagcgg caccagctcg acggcgagat
     2761 cgaccgctgg gtactgtcgc gcgtcatctc gcacttggcg cacgatgagg ccgcggtcga
     2821 gcggctgggc tatgtcggca tcaacctggc gccgtcgacc atcagcgact cgcatttcct
     2881 ggagttcctg gcgcagcagt tgcagcagct gcccaacctg ccgccgcgca agctgtgctt
     2941 tgaactgcgc gaatccgatg tgatcgatca cgcacgcgaa gcccaaatct tctgtgctgc
     3001 catgcgcagc atcggctgcc gcgtggccat cgaccacttc agcggacgca acagcagcac
     3061 gctggcgctg atccgcgggc tcggtgcgca gctggccaag atcgatgggc tggcctatgc
     3121 cgccatcgcc agcgacccgc tgcagcagca gctggccgaa tcggtgatca agctggcgcg
     3181 cgcgctgcac ctgcgggtgg cggtgtgcaa cctggagaac gggccgcagc tggaagcctg
     3241 gcgcaggctc ggcgccgact actaccaggg ccacgccatc gccaagcctt cgccgctggt
     3301 gttttcgccg ctgcgctaga gcggctcgat cagctcgatc agcaggtcgg ccgactcggt
     3361 gccattccag cgattgacgt tgagccggta gaggcagcgc acgcgccccg ccggcaccac
     3421 cgccggcgcg tggaaatgga tggctgcgcc gctgaagctg ccgctgcgca gccggtaacg
     3481 cgcgtggtcc tgctgctggc cgacggcgcg cgcttccacg atgtcgaact cgttctcgaa
     3541 cagcggcgcg gcgaagccct gcccccatgg cccggcctgt tccagcaggc gtgcggtgtc
     3601 gagattcagc tccgcatcgg ccagcgcacc gtcgctgtcc agc
//
LOCUS       DASZZC010000080         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1160608,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000080 DASZZC010000000
VERSION     DASZZC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160608"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VHE37_01935"
     CDS             <1..1357
                     /locus_tag="VHE37_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34317.1"
                     /translation="QDVLALSARVAQTLAPTLSLRNQLQFNHVLTEARETGANNVGTY
                     DAATGAFTPLPTAAQGNATALPYDQLYVKLASHDRRIDDESLFDQLDLRWCAQTGPVA
                     HDLIGGVELGRDTYSNQAYTRSTAAGYLAVVPLLNPPYLPQPANTVTTAGNVATANAG
                     TFGVYANDTVAFTPQWKLVAGVRWDQFRANLANSVNSTNTAGNTSVPAASQSVHFTSV
                     RIGGLYQPDAVQSYYVSYGTSFNPSLEQLTLTTGQQNLDPEKNRSYEVGAKFDLNDQA
                     LSLATALFRTEKTDARSLVSTGVYALDGDVRVQGAEISASGRLTGQWQLLAGYTWLDA
                     QIVQASAADATQGHVPANTPRHTAVLWSAYNITPAWEAGGGVNFMSARYASNTNTVSA
                     GSFARFDATVAYHQPHYDLRVNLLNLADRTYYAALIPSDAGRSVPGIGRTALLTATYK
                     F"
     gene            1370..2056
                     /locus_tag="VHE37_01940"
     CDS             1370..2056
                     /locus_tag="VHE37_01940"
                     /inference="COORDINATES: protein motif:HMM:NF003975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HVT34318.1"
                     /translation="MLVRIPQVLKSEQVAALRAKLDDASAPWVDGRVTAGHMGSKVKH
                     NSQIAEGSVLARELGEIVLGALERNAQFISAALPARVYPPMFNRYQPGMQFGDHVDNA
                     VRLIPGSALQIRTDISATLFLTAPEDYDGGELVVEDHYGHHSARLPAGDLLIYSADSV
                     HRVTPVTRGARVSCFFWVQSMVRDDAQRRLLYDLDRSVQRLNAAGADEQARLTLTGCY
                     HNLLRMWSHA"
     gene            2053..2670
                     /locus_tag="VHE37_01945"
     CDS             2053..2670
                     /locus_tag="VHE37_01945"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HVT34319.1"
                     /translation="MTRADALRLLRRWHGWIGLWGAGLGLLFGTTGILLNHRSVLKIP
                     AGATLDSTVQMALPTPAPADADALAAWLQQQLGLEHAATRVRQEPAHAVAWGVRSLQQ
                     PAHWSAAFTTPRMNLQADYWEGSTQVSVRRSHSNLWATLMNLHKGVGMGAAWVLLVDT
                     LAGSILLLSLSGVLMWTRLERARLFGAGVALLPAFAAATLALLNS"
     gene            complement(2679..3254)
                     /locus_tag="VHE37_01950"
     CDS             complement(2679..3254)
                     /locus_tag="VHE37_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34320.1"
                     /translation="MQTPHAIAQEAARIVCEDLVIDYAAAKKKALARLGLSPRAALPE
                     NAQVQQAVIEYQQLFGGARYRELLARMRSAALQAMTLLRDFSPCLAGALVSGAVTRAH
                     RVQLHAFSERAEAVEIFLGNRGHAVEQGERVYRYPGGREQQVAVVGFELADAGIDVAV
                     FDQEAAHQVPLNPLDGKSFQRLDSAAVRALG"
     gene            complement(3380..>4808)
                     /locus_tag="VHE37_01955"
     CDS             complement(3380..>4808)
                     /locus_tag="VHE37_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVT34321.1"
                     /translation="GPEYRLADEWLKRPAPDNDAIVEQLNELPELTDLPPAVVLEYKP
                     DAIKLLSASGLIDLPKSAFAWANIPPKKPLVRGDIVRIKRVGESWKLSQVPQAQGAFV
                     AVDPHDGAILALAGGYDFFLNKFNHVIQARRQVGSGFKPYLYCAAMAYGFTPASVFLD
                     APVVFDDPNLEQVWKPENYDRDYNGPMRLREALVQSRNLVSIRVLQAIGLDYAREYVT
                     RFGLPQDRLPRDLTMALGTAVFTPLEMARGYATIANGGFLVEPYFIDEIRDAGGAVLF
                     KADPKVACPECIEQNLQQAEDAAATQPPAEPAPPPVPPEKQAARTVDPQIVWLITSMM
                     HDVTVRGTGAAVNALGRTDLAGKTGTTNDETDAWFNGFQKGIVGISWVGFDQPTPLGK
                     GEVGGRASLPIWMDFMKSALKNVPQETLPRPPGLVDVRINPKTGKLALPDAPAIFETV
                     QQDRVPETDNGHEEEQEKSGVEDLY"
BASE COUNT          764 a         1629 c         1631 g          784 t
ORIGIN      
        1 gcaggacgtg ctggcgctgt cggcgcgtgt ggcgcagaca ctggcaccca cgctttcact
       61 gcgcaaccag ctgcagttca accacgtgct gaccgaggcg cgcgaaaccg gggccaacaa
      121 cgtcggcacc tacgacgccg ctaccggcgc gttcacgcca ttgccgacgg ccgcgcaggg
      181 caatgccacg gcgctgccgt acgaccagct gtacgtgaag ctggcttcgc atgatcgccg
      241 catcgacgac gaatcgttgt tcgaccagct tgacctgcgc tggtgcgcgc agaccgggcc
      301 ggtggcgcat gacctgatcg gcggcgtcga gctgggacgc gacacctact ccaaccaggc
      361 gtacacgcgc agcacggcgg ccggctatct cgcggtggtg ccgctgctca atccgccgta
      421 cctgccgcag ccggcgaaca ccgtcaccac cgccggcaac gtcgccaccg ccaatgccgg
      481 cactttcggc gtgtatgcca acgacaccgt ggcgttcacg ccgcagtgga aactggtggc
      541 cggcgtgcgc tgggaccagt tccgcgccaa cctggccaac agcgtcaaca gcacgaatac
      601 cgcgggcaat accagcgtgc cggcggcgtc gcaaagcgtg catttcacca gcgtgcgtat
      661 cggcgggctg tatcaacccg atgcggtgca gtcgtactac gtgtcgtacg gcacctcgtt
      721 caatccatcg ctggaacagc tgacgctgac caccgggcag cagaatctcg atcccgagaa
      781 aaaccgctcg tacgaggtcg gcgccaagtt cgatctcaac gaccaggcgc tgtcgctggc
      841 caccgcgctg ttccgcaccg agaagaccga tgcgcgctcg ctggtgtcca ccggcgtata
      901 cgcgctcgat ggcgacgtgc gcgtgcaggg cgccgagatt tccgccagcg gacgtttgac
      961 cgggcagtgg caactgctcg ccggctatac ctggctcgac gcgcaaatcg tgcaggcttc
     1021 ggcggccgat gccacgcagg gccacgtgcc ggccaacacg ccgcgccata ccgcggtgct
     1081 gtggagcgcg tacaacatca cgcccgcatg ggaagcgggc ggcggcgtca acttcatgtc
     1141 cgcgcgctat gcgtcgaaca ccaacacggt gtcggcggga agctttgcgc gcttcgacgc
     1201 gacggtggcc tatcaccagc cgcactacga cctgcgcgtg aacctgctca acctggccga
     1261 ccgcacgtac tatgcggcgc tgatcccgtc ggacgcgggc cgctcggtgc cgggcatcgg
     1321 ccgcaccgcg ctgctgacgg ccacgtacaa gttctagagg agcagccgca tgctggtgcg
     1381 cattccgcag gtgctgaaat ccgagcaggt ggcggcattg cgcgcgaagc tcgacgatgc
     1441 gtcggcgcca tgggtcgacg gccgcgtcac cgccgggcac atgggcagca aggtcaagca
     1501 caacagccag atcgccgagg gcagcgtgct cgcgcgcgag ctcggcgaga tcgtgctcgg
     1561 cgcactggag cgcaatgcgc agttcatcag cgcggcgctg ccggcgcgcg tatatccgcc
     1621 gatgttcaac cgctaccagc ccggcatgca gttcggcgat cacgtcgaca acgcggtgcg
     1681 gctgattccc ggcagcgcgc tgcagatacg caccgacatc tcggccacgc tgttcctgac
     1741 cgcgccggaa gactacgacg gcggcgagct ggtcgtggag gatcactacg ggcaccattc
     1801 ggcgcgcctg ccggccggcg acctgctgat ctactcggcc gacagcgtgc accgggtgac
     1861 gccggtgaca cgcggcgcgc gcgtgtcgtg tttcttctgg gtgcagagca tggtgcgcga
     1921 cgatgcgcag cgccgcctgc tgtacgacct cgaccgctcg gtgcagcggc tcaacgcggc
     1981 cggcgccgac gagcaggcgc ggctcacgct caccggctgc tatcacaacc tgctgcgcat
     2041 gtggagccac gcatgacgcg tgcggatgcg ctgcgcctgc tgcgccgctg gcatggctgg
     2101 atcggcctgt ggggtgcggg gctggggttg ttgttcggca ccactggcat cctgctcaac
     2161 caccgcagcg tgctgaagat tcctgcgggc gcaacgctgg attccacggt gcaaatggcg
     2221 ttgcccacgc cggcgcccgc cgacgcggat gcgctcgcgg cgtggctgca gcagcagctc
     2281 gggctcgagc acgcggccac gcgcgtcagg caggagccgg cgcacgcggt ggcctgggga
     2341 gtgcgctcgc tgcagcagcc ggcgcactgg agcgcggctt tcaccacgcc gcgcatgaac
     2401 ctgcaggccg attactggga aggcagcacg caggtcagcg tgcggcgcag ccacagcaac
     2461 ctgtgggcca cgctgatgaa cctgcacaag ggcgtgggca tgggcgcggc ctgggtgctg
     2521 ctggtcgaca cgctggccgg cagcatcctg ctgctgtcgc tgtccggcgt tttgatgtgg
     2581 acgcggctgg agcgcgcgcg cctgttcggc gccggcgtgg cgctgctgcc ggcgtttgcc
     2641 gccgcgaccc tcgcgctact gaattcgtag caggcggctc agccgagtgc gcgcaccgcg
     2701 gcggagtcga ggcgctggaa tgatttgccg tcgagcgggt tgagcggcac ctggtgcgcc
     2761 gcctcctggt cgaacacggc tacgtcgatg ccggcgtccg cgagttcaaa acccaccacc
     2821 gccacctgct gctcgcggcc tccgggataa cggtacacgc gctcaccctg ctcgacggca
     2881 tggccgcggt tgcccaggaa gatttccacg gcttccgcgc gctcgctgaa ggcatgcagc
     2941 tgcacgcggt gcgcgcgtgt gaccgcgccg ctgaccagcg cgccggccag gcacggcgag
     3001 aaatcccgga gcagcgtcat cgcctgcagc gccgcgctgc gcatgcgcgc cagcagctcg
     3061 cggtagcgcg cgccgccgaa cagctgctgg tactcgatca ccgcctgctg cacctgcgcg
     3121 ttctccggca gtgccgcgcg cggcgacagg cccagccgcg ccagcgcctt cttcttggcc
     3181 gcggcgtaat cgatcaccag gtcttcgcag acgatgcggg cggcttcctg ggcgatggca
     3241 tggggcgttt gcatggcgaa aaccggctta ccgcacggca attcttgatg ttataaaaaa
     3301 tatattataa ttaagattat acaataatta ataacacatg caatgccggt gcggtatggc
     3361 aaaaaattcg cgccgcgatt caatacagat cttcgacgcc ggatttttcc tgctcttcct
     3421 cgtggccgtt gtcggtctcc ggcacgcggt cctgctgcac cgtctcgaag atggccggcg
     3481 catcgggcag cgcgagcttg ccggtcttgg gattgatgcg cacgtcgacc aggcccggcg
     3541 gccgcggcag cgtttcctgc ggcacgttct tcagcgcgct cttcatgaag tccatccaga
     3601 tcggcaggct cgcgcgcccg ccgacttcgc ccttgcccag cggcgtgggc tggtcgaagc
     3661 cgacccagga aatgccgacg atgcctttct ggaaaccgtt gaaccacgcg tcggtctcgt
     3721 cgttggtggt gccggtcttg ccggcaagat cggtgcgccc gagcgcgttg accgccgcgc
     3781 cggtgccgcg cacggtgacg tcgtgcatca tgctggtgat cagccagacg atctgcggat
     3841 cgaccgtgcg cgcggcctgc ttctccggcg gcaccggcgg cggcgcgggc tcggctggcg
     3901 gctgcgtggc agcggcgtct tcggcctgct gcaggttctg ctcgatgcac tcgggacagg
     3961 ccactttcgg atcggccttg aacagcacgg cgccgccggc gtcgcggatc tcgtcgatga
     4021 agtacggctc caccaggaag ccgccgttgg cgatggtggc gtagccgcgc gccatctcca
     4081 gcggcgtgaa caccgcggtg cccagcgcca tggtcaggtc gcgcggcagg cggtcctgcg
     4141 gcaggccgaa gcgcgtcacg tactcgcgcg cgtagtcgag gccgatggcc tgcagaacgc
     4201 ggatcgacac caggttgcgc gactgcacca gcgcctcgcg caggcgcatc gggccgttgt
     4261 agtcgcggtc gtagttctcc ggtttccaca cctgctcgag gttggggtcg tcgaacacca
     4321 ccggcgcgtc gaggaacacc gaggccggcg tgaaaccgta cgccatcgcc gcgcagtaca
     4381 ggtacggctt gaagcccgag ccgacctggc gccgcgcctg gatcacgtgg ttgaacttgt
     4441 tgaggaagaa atcgtagccg ccggcgagtg ccaggatggc gccatcgtgc ggatcgaccg
     4501 cgacgaacgc gccctgcgcc tgcggcacct ggctcagctt ccagctctcg cccacgcgct
     4561 tgatgcgcac gatgtcgccg cgcaccagcg gtttcttcgg cgggatgttg gcccaggcaa
     4621 acgcggactt gggcaggtcg atcaggcccg acgccgacag cagcttgatc gcatcgggct
     4681 tgtactcgag caccaccgcc ggcggcagat cggtgagctc gggcagttcg ttgagctgct
     4741 cgacgatggc gtcgttgtcc ggcgccgggc gcttgagcca ttcgtcggcg aggcggtatt
     4801 cggggccg
//
LOCUS       DASZZC010000081         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_263458,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000081 DASZZC010000000
VERSION     DASZZC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263458"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..457)
                     /gene="gatC"
                     /locus_tag="VHE37_01960"
     CDS             complement(170..457)
                     /gene="gatC"
                     /locus_tag="VHE37_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVT34322.1"
                     /translation="MSLNADQVRQVAHLARLELKPEKVSVYATQLSNILQMVDRLSAA
                     DTSAVKEMAHPLDMQQRLRPDAVTETDQRELFQEHAPATQDGLYLVPKVIE"
     gene            580..1617
                     /locus_tag="VHE37_01965"
     CDS             580..1617
                     /locus_tag="VHE37_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVT34323.1"
                     /translation="MFDAVRRLFVNDLSIDLGTANTLVYVRDEGIVLNEPSVVSIRLD
                     QRGSKKIEAVGIDAKKMLGRTPNNISTIRPLRDGVIADYTVTEKMLQHFIKKVQKGRM
                     MRPITRVVVCVPYGSTQVERRAIRESVENAGARKVYVIEEPIAAALGAGIPIGEARGS
                     MVVDIGGGTSEVAVISLNGIVYAESVRIGGDKFDEAIVSYVRRQYNMLIGEATAERIK
                     QEIGTAFPGHEVQEIDVVGRHLAAGVPRNFTMNSNEILDALQEPLAGIVKAVKQALER
                     TPPELGSDVAERGIVLTGGGALLRDLDKLISEETGLPVIIADDPLTCVARGGGMVLDM
                     LDRQSDFYSME"
     gene            1812..2801
                     /gene="mreC"
                     /locus_tag="VHE37_01970"
     CDS             1812..2801
                     /gene="mreC"
                     /locus_tag="VHE37_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HVT34324.1"
                     /translation="MIFSCHSHYFYTTLNTVVISSHRRYFPRGPGLGLKALTLILCSL
                     ALIFADNRGDSLKPTREWATWALQPVIWLVSFPSQAATAPQGLQTRAALEAENKELRD
                     KQLVLEAQLTKFQALEAENRRIRELLSSAKNLSERVLIAEIVAINQDPYRHQITLNKG
                     TRDGVYRGQALVDATGVMGQIVEVGPGTSQALLITDPDHGIPVEINRTGLQTIALGRG
                     DGRGLTLPFLPSNADVKEGDLLVSSSLGGRFPSGYPVGQIYEVKHTAGEHFMAAAAYP
                     SAHLNQGRQALLVWSNDADAAAAAEAAAAAAAAEEARAARSNSATTATHKKKS"
     gene            2798..3286
                     /gene="mreD"
                     /locus_tag="VHE37_01975"
     CDS             2798..3286
                     /gene="mreD"
                     /locus_tag="VHE37_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HVT34325.1"
                     /translation="MIAGKTLYTAYGASLLAALLLQLVELPDALAGARPMWLPLVIAY
                     WALTEPRVSALVGAFLLGVVADVLFGSVLGEHALALVVLAYLVTRLRVIFALFPLWQA
                     TLALIPGWVVYAFMLFWIDGATHHQANPWLRWLPIVSTSLFWPLIYVTLEIMLGRREE
                     EE"
     gene            3429..>5392
                     /gene="mrdA"
                     /locus_tag="VHE37_01980"
     CDS             3429..>5392
                     /gene="mrdA"
                     /locus_tag="VHE37_01980"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVT34326.1"
                     /translation="MTPGRLGAFKDIQVEKQSFLGRIVFCGVLCLGLSILLVARLINL
                     QVVQHDYYTTRADDNRMRLQPVPPVRGLIYDRHGTLLAQNQPAFVLQVTPEQVDDIDQ
                     TLKDLQPIIALTDKDIARFKDRLKKTPRYRGVPLRSNLTMEEVARYEINRYNFHGVDV
                     TAGLTRNYPMGASSAHVVGYVGGITEEELATLPEKDYEGLTQIGKIGVEKSHEEQLRG
                     TPGAKIIEANAYGRPLRELDYRLGAPGKNLYLSIDARVQTAAETALGDLTGSIVAVDP
                     RNGEIIAMVSKPGFDPHLFVEGIDVPTYRALADDITRPLFDRTLQGQYPPGSTVKPAM
                     AIAGLEYGVLDPSHREFCRGEYTLPGSSRKYRCWKRHGHGSLDMTEGIMKSCDVYFYS
                     LANTLGIDRIHDAMSEFGLGKLTGVDLPLEKGGLVPSREWKRKRRKENWYAGETLSVG
                     IGQGYMIATPLQLAQMAARIAMRGAGYKPHVVHAMEDAQDKTFTEVAPEPLPPIKLKN
                     ARNWDEVINAMEQVAQSPGGTAYKIGHDAPYRIAAKTGTAQVAGMAQNEDEAKDISKV
                     PLQLRDHALIIAFAPADDPKIAVAVIAEHAGHGGTAAGPVARQVLDMALLGEVKFDPN
                     APARPAAAAPSAAPPAPVEEQEPDETPDSS"
BASE COUNT          978 a         1788 c         1757 g          869 t
ORIGIN      
        1 ggcgtcggcc tggcgcagcg cgcgttcgga ggtcacggtg atgcagctgt tgagcacgcc
       61 gtcgagccgg tcgatgcgct tcaagaaatg ctgcgtaagt tcgcgcgagg aaaacttctt
      121 cgcgcgcagc ccgtcggcga gctgctggat ggaaaggcgg tgcatggaat tactcgatga
      181 ccttgggcac caggtacagc ccgtcctgcg tggcgggcgc atgttcctgg aacagctcgc
      241 gctggtcggt ttcggtgacg gcgtcggggc gcaggcgctg ctgcatgtcc agcgggtgcg
      301 ccatctcctt caccgcggaa gtgtcggccg ccgagaggcg gtcgaccatc tgcaggatgt
      361 tcgacaactg ggtcgcgtag acggaaactt tttcaggctt gagttcgagc cgcgcgaggt
      421 gcgcaacctg ccggacctgg tctgcattca agctcataaa ttgctcattt tttctggctt
      481 aatgcgctat agtgtgcgcc gcacaattct acgggattgt gctcgccgcc cgcacctttg
      541 cggccgccca aaacaataga ccccgcaccc ccgacaagca tgttcgacgc cgtccgccgt
      601 ttgttcgtca acgacctgtc catcgacctg ggcaccgcca acacgctcgt ctacgtgcgc
      661 gatgaaggca tcgtgctcaa cgagccctcg gtggtgtcga tccgcctcga ccaacgcggc
      721 agcaagaaaa tcgaagccgt gggcatcgac gccaagaaga tgctgggccg cacgccgaac
      781 aacatatcca ccatccgccc gctgcgcgac ggcgtgatcg ccgactacac ggtcacggaa
      841 aaaatgctgc agcacttcat caagaaggtg cagaagggcc gcatgatgcg cccgatcacg
      901 cgcgtggtgg tgtgcgtgcc ctacggctcc acccaggtgg agcgccgcgc catccgcgag
      961 tcggtcgaga acgccggtgc gcgcaaggtt tacgtgatcg aggaaccgat cgccgcagcg
     1021 ctcggcgccg gcatcccgat cggcgaggcg cgcggctcca tggtggtgga catcggcggc
     1081 ggcaccagcg aagtcgcggt catctcgctc aacggcatcg tgtatgcgga atcggtacgc
     1141 atcggcggcg acaagttcga cgaggcgatc gtctcgtacg tgcgccgcca gtacaacatg
     1201 ctgatcggcg aagccaccgc cgagcgcatc aagcaggaaa tcggcacggc gttcccgggc
     1261 catgaagtgc aggagatcga cgtcgtcggc cgccacctgg ccgccggcgt gccgcgcaac
     1321 ttcaccatga actccaacga gatcctcgac gcgctgcagg agccgctggc cggcatcgtc
     1381 aaggcggtaa agcaggcgct ggagcgcacg ccgccggaac tgggttccga cgtggccgag
     1441 cgcggcatcg tgctcaccgg cggcggcgcg ctgctgcgcg acctggacaa gctcatctcc
     1501 gaggaaaccg gcctgccggt gatcattgcc gacgacccac tgacctgcgt ggcgcgcggc
     1561 ggcggcatgg tgctcgacat gctcgaccgc cagtccgact tctactcgat ggagtgagcg
     1621 caccggcggc gcacagctgc caccgcaccg cccgtttcga tgttattaaa gattgtctta
     1681 agttaatata agacaataat taaaaacaac ctggaacggc ttgcggtaga gcaaaaaatc
     1741 gcccctgcgc tgcatggaat ttcattgcgg cgacatgctt cgggggctac tctagcgtgg
     1801 ccatttgatg agtgatattc tcctgtcact cgcattactt ttatacaact ttgaacaccg
     1861 tcgtcatcag ctcacatcgc cgctattttc cgcgcgggcc agggctcggg ctgaaggcgt
     1921 tgacactgat tctctgctcg ctcgcgctga tcttcgccga caaccgcggc gactcgctca
     1981 agcccacgcg cgaatgggcc acctgggcgc tgcagccggt gatctggctg gtgtcgtttc
     2041 cgagccaggc cgccaccgct ccgcagggcc tgcagacgcg cgccgcactt gaagcggaga
     2101 acaaggaact gcgcgacaag cagctggtgc tggaagcgca gctgaccaag ttccaggcgc
     2161 tggaggcgga gaatcgccgc atccgcgaac tgctgtcgtc ggccaagaac ctgtccgagc
     2221 gcgtgctgat cgccgagatc gtggcgatca accaggatcc gtaccgtcac cagatcacgc
     2281 tgaacaaggg cacgcgcgac ggcgtgtacc gtgggcaggc gctggtcgat gccaccggcg
     2341 tgatgggcca gatcgtcgag gtcggcccgg gcacttcgca ggcgctgctg atcaccgatc
     2401 ccgaccacgg cattcccgtc gagatcaacc gcaccgggct gcagaccatc gcgctcggcc
     2461 gcggcgatgg ccgcgggctg acgctgccgt tcctgccgag caatgccgac gtgaaggaag
     2521 gcgacctgct ggtgtcgtcc tcgctcggcg gccgcttccc ctccggctac ccggtgggcc
     2581 agatctacga ggtcaagcac actgcgggcg agcacttcat ggccgccgcc gcatatccca
     2641 gcgcgcacct gaaccaggga cgccaggcgc tgctggtgtg gagcaatgat gccgatgccg
     2701 ccgccgctgc agaagcagct gcagcggctg cagccgccga ggaagcgcgc gccgcgcgca
     2761 gcaactccgc cacaacggcc acgcataaaa agaaatcgtg atcgcgggca agacgctcta
     2821 caccgcctac ggcgcgagcc tgctggcggc gctgctgttg caactggtgg agctgcccga
     2881 cgcactggcc ggcgcgcggc ccatgtggct gccgctggtc atcgcctact gggccttgac
     2941 cgagccgcgc gtatcagccc tggtcggcgc cttcctgctc ggcgtggtgg ccgacgtatt
     3001 gttcggatcg gtgctcggcg agcatgcgct ggcgctggtg gtgctggcct acctggtgac
     3061 gcggctgcgg gtgatcttcg cgctgtttcc attgtggcag gccacgctgg cactgattcc
     3121 cggctgggtg gtctacgcct tcatgctgtt ctggatcgac ggagcgacgc atcaccaggc
     3181 caacccgtgg ctgcgctggc tgcccatcgt ctccacctcg ctgttctggc cgctgatcta
     3241 cgtcacgctg gagatcatgc tgggccggcg cgaagaggaa gaatgatgga agcctcacaa
     3301 agctgctgcg ctcggcatct tgcgcgcggg cggtgctcgc aatcctcatt gactgacgtg
     3361 tcaactccgg ctgctccgct ccggctgggc gcaacctgcc gtcgctcgcg acgctttgtg
     3421 aggcttccat gacgccaggc cggctcggcg cattcaagga catccaggtc gagaagcagt
     3481 cgtttctcgg ccgcatcgtg ttctgcggcg tgctgtgcct gggcctgtcc atcctgctgg
     3541 tggcgcggct gatcaacctg caggtggtgc agcacgacta ctacaccacg cgcgccgacg
     3601 acaaccgcat gcgcctgcag ccggtgccgc cggtgcgcgg actgatctac gaccgccatg
     3661 gcacgctgct ggcgcagaac cagccggcct tcgtgctgca ggtgacgccg gaacaggtcg
     3721 acgacatcga ccagacgctc aaggacctgc agccgatcat cgcgctcacc gacaaggaca
     3781 tcgcgcgctt caaggaccgc ctgaagaaga cgccgcgcta ccgcggcgtg ccgctgcgct
     3841 ccaacctgac catggaggag gtggcgcgct acgagatcaa ccgctacaac ttccacggcg
     3901 tcgacgtcac tgccggcctc acgcgcaact acccgatggg tgcttcctcg gcgcacgtgg
     3961 tcggctatgt cggcggcatc accgaggaag agctggccac gctgccggag aaggattacg
     4021 aaggcctgac gcagatcggc aagatcggcg tggagaagag ccacgaggaa cagctgcgcg
     4081 gcacgcccgg cgccaagatc atcgaggcca atgcctatgg ccggccgctg cgcgagctcg
     4141 actaccggct cggcgcaccg ggcaagaacc tgtacctgtc gatcgacgct cgcgtgcaga
     4201 ccgccgccga gacggcgctg ggcgacctga ccggatcgat cgtggcggtt gatccgcgca
     4261 acggcgagat catcgccatg gtcagcaagc cgggcttcga cccgcacctg ttcgttgaag
     4321 gcatcgacgt gcccacctac cgcgcgctgg ccgacgacat cacgcgcccg ctgttcgacc
     4381 gcacgctgca gggccagtac ccgccgggct ccacggtgaa gccggccatg gccatcgccg
     4441 gcctggagta cggcgtgctc gatccctcgc accgcgagtt ctgtcgcggc gagtacacgc
     4501 tgcccggttc ctcgcgcaag taccgctgct ggaaacgcca cggccacggc tcgctggaca
     4561 tgaccgaagg catcatgaag tcgtgcgacg tgtacttcta ttcgctggcc aatacgctgg
     4621 gcatcgaccg catccatgac gccatgagcg agttcggcct gggcaagctc accggcgtcg
     4681 acctgccgct ggagaaaggc gggctggtgc cgtcgcgcga gtggaaacgc aagcggcgca
     4741 aggagaactg gtacgccggc gaaacattgt cggtcggcat cggccagggc tacatgatcg
     4801 ccacgccgct gcagctggcg cagatggccg cgcgcatcgc catgcgcggc gccggctaca
     4861 agccgcacgt ggtgcacgcc atggaagatg cgcaggacaa gacctttacc gaggtggcgc
     4921 cggaaccatt gccgccgatc aagctcaaaa acgcgcgtaa ctgggacgag gtgatcaacg
     4981 cgatggaaca ggtggcgcaa tcgcctggag gcaccgccta caagatcggc cacgacgcgc
     5041 cgtaccgcat cgcggccaag accggcaccg cgcaggtggc cggcatggcg cagaacgagg
     5101 acgaggccaa ggacatctcc aaggtgccgc tgcaactgcg cgaccacgcg ctgatcatcg
     5161 ccttcgcgcc ggccgacgat cccaagatcg cggtggcggt gatcgccgag cacgccggcc
     5221 acggcggcac cgcggccggc ccggtggcgc gccaggtgct ggacatggcg ctgctgggcg
     5281 aagtgaagtt cgaccccaac gcgccggccc ggccggctgc cgccgcgccg agcgcagcgc
     5341 caccggcgcc ggtggaagag caggagccgg acgaaacacc cgacagcagc ga
//
LOCUS       DASZZC010000082         8170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_263647,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000082 DASZZC010000000
VERSION     DASZZC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263647"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="pstB"
                     /locus_tag="VHE37_01985"
     CDS             complement(<1..710)
                     /gene="pstB"
                     /locus_tag="VHE37_01985"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HVT34327.1"
                     /translation="MASVMDLPQDAGTSAPEKISIRDLSFYYGETQALKHITLPLLER
                     NVTAFIGPSGCGKSTLLRVLNRMYDLYPGQRVTGQVLLNGKDDILAPAQDLNLLRARI
                     GMVFQKPTPFPMSIYDNIAFGVRLYEKLSRVNMDERVEESLRRTALWDEVKTKLHSSG
                     MSLSGGQQQRLCIARTIAVRPQVILLDEPTSALDPISTAKIEELIYQLKNDYTLVIVT
                     HNMQQAARVADYTAFMYLG"
     gene            complement(757..1617)
                     /gene="pstA"
                     /locus_tag="VHE37_01990"
     CDS             complement(757..1617)
                     /gene="pstA"
                     /locus_tag="VHE37_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HVT34328.1"
                     /translation="MTSADTTSIYRRRRRGNAIAMALAYLATSMGLLALAWILGTLLW
                     KGLGNLSLATFSEMTPPPGSQGGLLNAIYGSAVQTLIATVIGTPIGILAGTYMAEYGR
                     GSKLATTVRFINDVLLSAPSIVVGMFVYEIAVSPVGHFSAWAGCVALAILLMPVVIRT
                     TEDMLLLVPDSLREAASALGAPRYLVIRRICYAAARAGLVTGVLLAVARISGETAPLL
                     FTSLNNQFWSTHMNGPMANLPVVIFQFALSPYVDWQRLAWAGALIITTTVLVLNITAR
                     ALVSSRSAPR"
     gene            complement(1617..2600)
                     /gene="pstC"
                     /locus_tag="VHE37_01995"
     CDS             complement(1617..2600)
                     /gene="pstC"
                     /locus_tag="VHE37_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HVT34329.1"
                     /translation="MNTASDAAIPMSGASDSALARNSLRDAIARWATASAAVLVLLML
                     GGVMVSLIHGSMPSIKAYGFAFLTTESWNPVTEKFGAAAPIYGTVVTSLIAMIIAIPV
                     GIGIAIFITELCPRWLKRPIGTAIELLADIPSIIYGIWGLFILGPLLQQYVQPALINF
                     FDGVPWLSSLFAGPPYGIGVLTAGVILSIMVLPFITSITRDVFETVPPMLKESAYGLG
                     CTTWEVVRNVVLPYTRTGVIGGIMLGLGRALGETMAVTFVIGNAHRIGSSLLAPGTTI
                     SASIANEFTEADGVLYTSSLMELGLILFVITFIVLAAARLLLLRLQQKAGN"
     gene            complement(2679..3734)
                     /gene="pstS"
                     /locus_tag="VHE37_02000"
     CDS             complement(2679..3734)
                     /gene="pstS"
                     /locus_tag="VHE37_02000"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HVT34330.1"
                     /translation="MKKISTISWLRAAAAGAAMLAGVQASAADISGAGATFPYPLYSK
                     WADQYKKETGIGLNYQSIGSGGGIKQIKAKTVTFGASDKPLKVDDLDAAGLMQWPMVM
                     GGVVAVINVKGINTGDLVLDGPTLANIFMGKVTKWNDSAIAKLNPKLALPATAIAVVH
                     RSDGSGTTFNFAYYLGQVSSTWKDVVGVDASVEWPVGIGGKGNEGVANMVTQTDGSIG
                     YVEYAYALQNKMNYARMINKDGKTVSPSDATFKAAAANADWSKAPGYYLILANQPGAD
                     SWPMTAATFILVYKKPDDAAAVLEALKFFDWCYKKGEKMALDLDYVPMPAAVVQMVQK
                     SWGQITDSSGKPVWQSK"
     gene            3957..5147
                     /locus_tag="VHE37_02005"
     CDS             3957..5147
                     /locus_tag="VHE37_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HVT34331.1"
                     /translation="MNKLLKIGAIASAIAAMYSTNALADSASTKGGLTIKSDDGRFEG
                     TLGGRLQYDYTAFQADNEAVGGHTTTADGPYMRRAYITLSGKIYDFKYKLENDFAGGS
                     TGYASKELWIGHDLFGGFIKAGHMQPAYGMEELTSSNELLFTERPFISNNTVFSGREF
                     QNGVSYNADGHGMTGMVTIYNANTADDKATGANPVNGSGYSGRGTWAPINDNGSVLHL
                     GLAIDEAKFSDGTLPAAVSALYAEKNGSKITVASAGYKSQTTFVGELAGAVGPFTAQA
                     EYANAKYKYPAAADQTLDSFYVQASWMITGESKPYKAAAGVFGNPKPNSVDGAWEAKV
                     RYDYVKNKDLATAPKASEISLGVNYYFNDKVRMMLDYNIGNGETTAGKDKPKALVARA
                     QLAF"
     gene            5242..5958
                     /gene="phoU"
                     /locus_tag="VHE37_02010"
     CDS             5242..5958
                     /gene="phoU"
                     /locus_tag="VHE37_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HVT34332.1"
                     /translation="MDKALHRQHISSQFNAELEDVRQRVLAMGGLVEHQIAEATRALT
                     EGDVALAEQVARSDYKVNQFEVLIDEECSRILARRQPAAGDLRLLVAAIKTITDLERI
                     GDEAEKIARMAKDLASQERNSSAMVEVTHLSKHVSQMVRDALDSFARMDAEAALAVAR
                     EDANVDREYEALMRQCITFMMEDPRTIRRVMDIIWSVRALERIGDHAKNIAEYVIYLV
                     KGKDVRHVSIEDMEREVKGQ"
     gene            complement(5963..6970)
                     /locus_tag="VHE37_02015"
     CDS             complement(5963..6970)
                     /locus_tag="VHE37_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVT34333.1"
                     /translation="MTELHIGIYAIVFLIALALAFDFMNGFHDAANSIATIVSTGAMR
                     PQAAIVWAAFFNFIAFLFFGFHVAATIGKGIIDPAIVDARVIFGALTGAISWNILTWY
                     YGIPSSSSHALVGGLAGAAVAKSGSGALIGSGLYKVIGAIVLSPLLGFLLGGLLMVAV
                     AWIGRMSTPRRMERWFHSLQYISAAAYSMGHGSNDAQKTMGIIWMLLIAAGQAGSSGD
                     PPFWVVLACQTAMGLGTMFGGWRIVKTMGMRITKLKPVGGFCASASGAATLFLATGLG
                     IPVSTTHTITGAIVGVGSSRKVSAVRWGVAGNIVWAWIFTIPASAFISAVAWALSRAL
                     F"
     gene            complement(6981..7592)
                     /locus_tag="VHE37_02020"
     CDS             complement(6981..7592)
                     /locus_tag="VHE37_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HVT34334.1"
                     /translation="MPKEGRFFDYFSEHAEQLVLGAVELKVLMGNVADLPARKRNIES
                     IEHKADKITQAVMQLLHHTFITPLDRDEIHQLITRMDDILDLMEDVSQCMFLYDIKAV
                     TAEAAQLADICLVSCEKVRAAVRLLSDMKHAQTILPLCAEIDRLESDADHVMRSAMAK
                     LFREETDTRQLFKMKEIYQLLESVTDRCQDVANIIEGIVLENA"
     gene            7825..>8170
                     /locus_tag="VHE37_02025"
     CDS             7825..>8170
                     /locus_tag="VHE37_02025"
                     /inference="COORDINATES: protein motif:HMM:NF026164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34335.1"
                     /translation="MARKKSFPYRRTLIAAALVLASAGIVALTLFLLSLDHDIRARFA
                     GSRWALPAQVYAAPLELYPGLQLDADGLRRELVRLGYRAVSELDGPGTFTAGNARIEI
                     HTRAFNFWDGPQG"
BASE COUNT         1548 a         2550 c         2642 g         1430 t
ORIGIN      
        1 cccaggtaca tgaatgcggt gtagtcggcc acgcgcgcgg cctgctgcat gttgtgggtg
       61 acgatgacca gcgtgtagtc gttcttcagc tggtagatca actcttcgat cttggcagtg
      121 gagatcgggt ccagagccga ggtgggttcg tcgagcagga tcacctgcgg gcgcacggcg
      181 atggtgcgcg cgatgcacag gcgctgctgc tggccgcccg acaggctcat gccgctggaa
      241 tgcagcttgg tttttacttc atcccacagc gcggtgcggc gcagcgattc ctcgacgcgc
      301 tcgtccatgt tcacgcgcga cagcttctcg tacagccgta cgccgaaggc gatgttgtcg
      361 tagatcgaca tcgggaacgg cgtcggcttc tggaacacca tgccgatgcg cgcgcgcagc
      421 aggttcaagt cctgcgccgg cgcaaggatg tcgtccttgc cgttgagcag cacctggccg
      481 gtaacgcgct ggccggggta caggtcatac atgcggttga ggacgcgcag cagcgtcgac
      541 ttgccgcagc cggacgggcc gatgaaggcg gtgacgttgc gttccagcaa cggcagcgtg
      601 atgtgcttga gcgcctgcgt ctcaccgtaa tagaacgaca gatcgcggat ggagatcttc
      661 tccggcgcgc tggtgcccgc atcctgcggc aggtccatca ctgaggccat cggaagaggt
      721 gggcgttctt tcatggtgtt gttcatgagc gtgggttcag cgcggcgcgc tgcgcgagga
      781 aaccagcgcg cgtgcggtga tgttgagaac caggaccgtg gtggtgatga tcagcgcgcc
      841 ggcccaggcc agccgctgcc agtccacgta gggcgacagc gcgaactgga agatgaccac
      901 cggcaggttg gccatcggcc cgttcatgtg cgtgctccag aactggttgt tgagcgatgt
      961 gaacagcagc ggcgcggttt cgccactgat gcgcgccacc gccagcagca caccagtgac
     1021 caggccggcg cgcgcggcgg cgtagcagat gcgccggatc accaggtagc gtggtgcgcc
     1081 gagcgcggag gccgcttcgc gcaggctgtc gggcaccagc agcagcatgt cttcggtggt
     1141 gcggatgacc accggcatga gcaggatggc cagggccacg caaccggccc aggcggaaaa
     1201 atgcccgacc gggctcaccg cgatttcata gacgaacatg ccgaccacga tcgatggcgc
     1261 cgacagcagc acgtcattga tgaagcgcac cgtggtggcg agcttgctgc cgcggccgta
     1321 ctcggccatg taggtgccgg ccaggatgcc aatcggcgtg ccgatgacgg tggcgatcag
     1381 tgtctgcacc gcgctgccgt aaatcgcgtt gagcaaaccg ccctggctgc cgggcggcgg
     1441 cgtcatctcg ctgaatgttg ccagcgagag attgcccagg cccttccaca gcagcgtgcc
     1501 cagaatccag gccagtgcca gcaagcccat cgaggtcgcc aggtacgcga gcgccatggc
     1561 aatggcattg ccgcgccggc ggcgccggta aatcgaggtg gtatccgcgc tggtcatcag
     1621 ttgcctgcct tctgctgcag gcgcagcagc aacagccgtg ctgctgcgag cacgatgaag
     1681 gtaatgacga acaggatcag tccgagttcc atcagtgatg acgtgtaaag cacgccgtcg
     1741 gcctcggtga attcgttggc gatgctggcg gagatggtcg tgccgggcgc cagcagcgaa
     1801 ctgccgatcc tgtgcgcgtt gccgatgacg aacgttaccg ccatggtttc tccgagcgca
     1861 cggcccaggc cgagcatgat gccgccgatg acgccggtgc gcgtataggg cagcaccacg
     1921 ttgcgcacga cttcccaggt ggtgcagccc aggccgtagg ccgattcctt cagcatgggc
     1981 ggaacggttt cgaacacgtc gcgcgtgatc gaggtgatga acggcaatac catgatcgac
     2041 aggatcacgc cggcggtgag cacaccgatg ccgtagggcg gtccggcaaa caggctggac
     2101 agccacggca cgccatcgaa gaagttgatc aacgcaggct gcacgtactg ctgcagcagc
     2161 gggccgagga tgaatagccc ccagatgccg tagatgatgc tgggaatatc ggccagcagt
     2221 tcaatggcgg taccgatcgg acgcttgagc cagcgcgggc acagttcggt gatgaagatg
     2281 gcaatgccga tgccgaccgg gatggcgatg atcatggcaa tcagcgaggt caccacggtg
     2341 ccgtagatcg gcgctgcggc gccaaacttt tcggtcaccg ggttccagct ttccgtggtc
     2401 aggaaggcaa agccataggc cttgatcgac ggcatggagc catgtatcag ggacaccatc
     2461 acgccgccga gcatcagcag caccagcacc gcggccgaag ccgtggccca gcgtgcgatg
     2521 gcatcgcgca ggctgttgcg cgccagcgcc gagtcgctgg caccgctcat ggggatggct
     2581 gcgtcggaag cagtattcac gcgaactccg gccgcgcggg cggcctctcg gtggaaaaaa
     2641 ggcgggagcc ggtccggctc ccgcccgcag catgcatctt acttggactg ccagaccggc
     2701 ttgccggagc tgtcggtgat ctggccccag ctcttctgca ccatctgcac gaccgcggcc
     2761 ggcatcggca cgtagtcgag gtccagcgcc atcttctcgc ccttcttgta gcaccagtcg
     2821 aagaacttca gcgcttcgag cacggcagca gcgtcatccg gcttcttgta gacgaggatg
     2881 aaggttgcgg cggtcatcgg ccagctgtcg gcgccaggct ggttggccag gatcaggtag
     2941 taacccggcg ccttgctcca gtcggcgttg gcggcggcgg ccttgaaggt cgcatcgctc
     3001 ggcgacaccg tcttgccgtc cttgttgatc atgcgcgcgt agttcatctt gttctgcagc
     3061 gcgtaggcgt attcgacgta gccgatcgag ccgtccgtct gcgtgaccat gttggccacg
     3121 ccttcgttac ccttgccgcc gatgcccacc ggccattcga ccgaggcatc aacgccgacc
     3181 acgtccttcc aggtgctgct gacctgcccg aggtaatagg cgaagttgaa ggtggtgccg
     3241 gaaccgtcag agcggtgcac cacggcgatg gcggtggccg gcagcgccag cttggggttg
     3301 agcttggcga tcgcgctgtc gttccacttg gtgaccttgc ccatgaagat attggccagc
     3361 gtcgggccgt cgaggaccag gtcaccggtg ttgatgccct tgacgttgat cacggcgacc
     3421 acgccgccca tcaccatcgg ccactgcatc aggccggcgg cgtcgaggtc atccaccttc
     3481 agcggcttgt cgctggcgcc gaaggtcacc gtcttggcct tgatctgctt gatgccgcca
     3541 cccgagccga tcgactggta attgaggccg attccggttt ccttcttgta ctggtccgcc
     3601 cacttggaat agagcgggta ggggaaggtc gcgcccgcgc cggaaatatc ggctgcggaa
     3661 gcctgtacgc cagccagcat ggctgctcct gcggcggctg cacgaagcca ggaaatggtc
     3721 gagatttttt tcacgcactg ctcccaagtg gatggttcag gatgcctgat ttgcgaggcg
     3781 gcgcgactct acgcggctag catgacagtt ccgttgcagt cgcacacaaa gggtgctgta
     3841 acaaatatga aacagacccg tcacgaagta gacaccatcc tcggcttcaa tgccgctcgc
     3901 ttcgaggacc tagggtccat cactggcttt ttttcatgca ctgggagacg aacaacgtga
     3961 acaagctgct caagattggt gcgattgcct ctgcgattgc tgccatgtat agcaccaacg
     4021 ccctggcgga ttccgcaagc accaagggcg gcctgaccat caagtctgac gacggccgtt
     4081 ttgaaggcac gctgggcggc cgcctgcagt atgactacac cgcgttccag gccgacaacg
     4141 aagcggttgg tggccacacc accaccgctg acggcccgta catgcgccgt gcgtacatca
     4201 cgctctccgg caagatctac gacttcaagt acaagctgga aaacgacttc gccggcggct
     4261 ccaccggcta tgcgagcaaa gagctctgga tcggccacga cctgttcggc ggcttcatca
     4321 aggccggtca catgcagccg gcttacggca tggaagaact gaccagctcg aatgaactcc
     4381 tcttcaccga gcgcccgttc atcagcaaca acacggtgtt cagcggccgc gagttccaga
     4441 acggcgtgtc gtacaacgcc gatggccacg gcatgaccgg catggtcacc atctacaacg
     4501 ccaacaccgc tgacgacaag gcaaccggcg ccaatccggt caacggttcg ggctattccg
     4561 gccgcggcac ctgggcgccg atcaacgaca acggctcggt gctgcacctg ggcctggcca
     4621 tcgacgaagc caagttctcc gacggcacgc tgccggctgc cgtgtcggcg ctgtatgccg
     4681 agaagaacgg ctcgaagatc accgttgcca gcgcgggtta caagagccag acgacctttg
     4741 ttggcgaact ggcgggcgcg gtcggcccgt tcacggcgca ggctgaatac gccaatgcga
     4801 agtacaagta cccggcggca gcggaccaga cgctggactc gttctacgtg caggccagct
     4861 ggatgatcac cggcgagtcc aagccgtaca aggcggcggc cggcgtgttc ggcaatccga
     4921 agccgaacag cgtcgacggt gcgtgggaag ccaaggtgcg ctacgactac gtgaagaaca
     4981 aggatctggc cacggctccc aaggccagcg agatcagcct gggcgtcaac tactacttca
     5041 acgacaaggt ccgcatgatg ctggactaca acatcggcaa cggcgaaacc accgccggca
     5101 aggacaagcc gaaggcgctg gtcgcccgcg ctcagctggc gttctgatgt gtcgggtgtc
     5161 cggcgccgcc aggcgccgga cgcccatccc ttcatcgctc atggacgtgc gatagtaaga
     5221 gcgagcaact gaagaccggc catggacaaa gcactgcatc gccaacatat ctccagccag
     5281 ttcaacgccg agctggaaga cgtgcgccag cgcgtgctgg ccatgggcgg cctggtcgag
     5341 caccagatcg ccgaggccac gcgtgcgctg accgaaggcg acgtggcgct ggccgagcag
     5401 gtggcgcgca gcgattacaa ggtcaaccag ttcgaagtcc tgatcgacga ggagtgcagc
     5461 cgcatcctcg cgcgacgcca gccggccgcc ggcgacctgc gcctgctggt ggcggcgatc
     5521 aagaccatca ccgatctcga gcgcatcggc gacgaggcgg agaagatcgc acgcatggcc
     5581 aaggacctgg cctcgcagga gcgcaattcc tcggccatgg tcgaggtcac gcacctgagc
     5641 aagcacgtgt cgcagatggt gcgcgacgcg ctggattcgt tcgcgcgcat ggacgcggaa
     5701 gcggcgctgg cggtggcgcg tgaagatgcc aacgtcgacc gcgaatacga ggcgctgatg
     5761 cgccagtgca tcaccttcat gatggaagat ccgcgcacca tccgccgggt gatggacatc
     5821 atctggagcg tgcgcgcgct cgagcgcatc ggcgatcacg ccaagaacat cgccgagtac
     5881 gtgatctacc tggtcaaggg caaggacgtg cgccacgtgt ccatcgagga catggagcgc
     5941 gaggtcaagg gccagtaacg gctcagaaca gggcgcgcga cagcgcccac gccaccgccg
     6001 agatgaaggc gctggcgggg atggtgaaga tccacgccca tacgatgttg ccggccacgc
     6061 cccagcgcac ggccgatact ttccgcgacg agcccacgcc gacgatggcg ccggtgatgg
     6121 tgtgcgtggt cgataccggt atgcccagcc cggtggcgag gaacaaggtt gccgcgccgc
     6181 tggccgaggc gcagaagccg ccgaccggtt tcagcttggt gatgcgcatg cccatggtct
     6241 tgacgatgcg ccagccgccg aacatggtcc ccagccccat cgcggtctga caggccagca
     6301 ccacccagaa cggcggatcg ccgctgctgc cggcctgccc ggcggcgatc agcagcatcc
     6361 agataatgcc catggtcttc tgcgcatcgt tgctgccatg acccatgctg taagccgcag
     6421 cggaaatgta ttgcaggctg tggaaccagc gctccatcct gcgcggtgtc gacatacggc
     6481 cgatccatgc caccgccacc atcagcaagc cgccgagcag gaagcccagc agcggcgaca
     6541 gcacgatggc gccgatcacc ttgtacaggc cactgccgat cagcgcgccg ctgccgctct
     6601 tggcgactgc ggcgccggcc agcccgccga ccagcgcgtg cgacgagctg gagggaatgc
     6661 cgtagtacca ggtgaggatg ttccagctga tggcgccggt cagcgcgccg aagatcacgc
     6721 gcgcgtcgac aatggccgga tcgatgatgc ccttgccgat ggtcgccgcc acgtggaagc
     6781 cgaagaacag gaaggcgatg aaattgaaga acgcagccca gacgatggcc gcctgcggcc
     6841 gcatggcgcc ggtggagacg atggtggcga tggagttggc ggcgtcgtgg aagccgttca
     6901 tgaaatcgaa ggccaacgcc agcgcgatca ggaacacgat cgcgtagatg ccgatgtgca
     6961 gttctgtcat cgggctgcgc tcaggcgttc tcgagcacga tgccttcgat gatgttggcc
     7021 acgtcctggc aacgatcggt gaccgactcc agcagctggt aaatctcctt catcttgaac
     7081 agctgccgcg tgtcggtttc ctcgcggaac agcttggcca tggcgctgcg catcacgtgg
     7141 tcggcatcgg attccagccg gtcgatctcc gcgcacagcg gcaggatggt ctgcgcgtgc
     7201 ttcatgtcgg acagcagcct gaccgcggca cgcaccttct cgcagctgac caggcagatg
     7261 tcggccagtt gcgccgcctc ggccgtcacc gccttgatgt cgtagaggaa catgcactgc
     7321 gacacgtctt ccatcaggtc gaggatgtcg tccatgcgcg tgatcagctg gtggatctcg
     7381 tcgcggtcca gcggcgtgat gaaggtgtga tgcaacagct gcatcaccgc ctgggtgatc
     7441 ttgtcggcct tgtgctcgat gctctcgatg ttgcgcttgc gtgcgggcag gtcggctacg
     7501 ttgcccatca gcaccttcag ttccaccgcg cccagcacca gctgttcggc atgttcggag
     7561 aagtagtcga agaagcgccc ttctttgggc atcagccgtc cgaacatagg ttcatccctg
     7621 tttttttgat caggcgcgat tctactgacg tgttgcaaat tcatgtcaaa acgatgatga
     7681 gctgcttgcg ggccgctacc gcaccgcctg tggtgatgtt agtaaatata caataaaata
     7741 ataaatgaat aatatttaat aacaacaaaa tcccgggtgc ggtggccacg aaaacgtggc
     7801 tgcccggtat catgcgcccc ctttatggcg cgcaagaaat ctttcccgta ccggcgcacg
     7861 ctgatcgccg ccgcgctggt gctcgcctcg gccggcatcg ttgcgctcac gctcttcctg
     7921 ctgtcgctcg atcacgatat ccgtgcgcgc ttcgccggtt cgcgctgggc gttgccggcc
     7981 caggtgtatg ccgcgccgct ggagttgtat cccggcctgc agctcgacgc cgatggcctg
     8041 cgccgcgaac tggtgcgcct gggctaccgc gcggtgagcg agctcgacgg ccccggcacc
     8101 ttcaccgccg gcaacgcccg tatcgagatc cacacgcgcg cattcaactt ctgggacggc
     8161 ccgcagggcg
//
LOCUS       DASZZC010000083         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_647290,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000083 DASZZC010000000
VERSION     DASZZC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647290"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VHE37_02030"
     CDS             <1..1447
                     /locus_tag="VHE37_02030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HVT34336.1"
                     /translation="ADDDPLLRQVTSTSDNCLGSKCPDFSSCFVAKARRAAQAADVVV
                     INHHLLFADYLLKEEGFGQILSGVDVCIIDEAHQLPELAAAFFGVRLSTRQLHELCKD
                     TETEAGKLADIAHVGECVAELGRRAATLESGFAQMPNRITLHEFLNNGRVSEAVQAAR
                     DALQALYESIKPYEERSAEMASCVARAAALVERLQTILDEQDHERVRWVEALGRGGSL
                     HATPILVAEGFRRMAATYPGAWIFTSATLAASEDFSHFSGQLGLDQARELKLDSPFDF
                     STQARLYLPKGMPDPSDPAYSDAVADAALPLIQASGGGAFVLCTSHRALRRIAERLRP
                     KLKQKLYVQGDDSRAALLQAFAADGDGVLIGTSSFWEGVDVKGRALRLVIIDKLPFTA
                     PGDPVFEAKLAAIRAGGGNPFNDFQVPQAITALRQGVGRLIRDAADRGLLALCDPRLR
                     SKMYGRRMLASLPQMPVLSELADAQQWLRSL"
     gene            1444..2145
                     /gene="tsaB"
                     /locus_tag="VHE37_02035"
     CDS             1444..2145
                     /gene="tsaB"
                     /locus_tag="VHE37_02035"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HVT34337.1"
                     /translation="MKLLAIDTATEACSVALHLDGVVSERFVNAGRSHTELLLPMVRG
                     LMADAGIAFGQLDGIACGIGPGSFAGVRIGVGYVKGAALAADLPVVGISSLAMLAQGA
                     IAQGHARVLPVIDAKTNEIYAGLYERDAQGLARAVMPDLVCLPAELPATEGECHGCGS
                     GWGTYGEILRTRARVTASDAAAQPHASAALALALPEFAAGRGIGADELVPAYLRNKVA
                     LTLLEQADLRRRNKG"
     gene            2159..2255
                     /gene="ffs"
                     /locus_tag="VHE37_02040"
     ncRNA           2159..2255
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHE37_02040"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2421..>3241
                     /gene="dnaX"
                     /locus_tag="VHE37_02045"
     CDS             2421..>3241
                     /gene="dnaX"
                     /locus_tag="VHE37_02045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HVT34338.1"
                     /translation="MSYMALARKWRPRKFEDVAGQDHVVKALSHALDTDRLHPAILLT
                     GTRGVGKTTLARIIAKGLNCEKGVSAHPCGVCASCREIDEGRSVDMLEIDAASNTGVD
                     NIRELIENSQYAPARGRYKVYLIDEVHMLSKGAFNALLKTLEEPPPHIKFILATTDPQ
                     KLPVTVLSRCLQFNLKRLPQVLIRAQMDKVLASENVQADAGAVAELARAADGSMRDGL
                     SLLDQAIAYGGGKVEEAEVRAMLGTIEHDHVTQLVEALAAGDGPVLLNRIAALNE"
BASE COUNT          521 a         1081 c         1132 g          507 t
ORIGIN      
        1 cgccgacgac gatccgctgc tgcgccaggt cacctcgacc tccgacaact gcctgggcag
       61 caagtgcccc gatttctcct cgtgcttcgt ggccaaggcg cggcgtgcgg cgcaggcggc
      121 cgatgtggtg gtgatcaacc atcacctgct gttcgccgat tacctgctca aggaggaagg
      181 cttcggccag atcctgtcgg gcgtcgacgt ctgcatcatc gacgaggcgc accagctgcc
      241 ggaactcgcg gcggctttct tcggcgtgcg cctgtccacg cgccagctgc acgagctgtg
      301 caaggacacc gagacggaag cgggcaagct ggccgacatc gcgcacgtgg gcgaatgcgt
      361 ggccgagctg ggcaggcgcg cggccacgct ggaatccggt tttgcgcaga tgcccaaccg
      421 catcacgctg catgagttcc tcaacaacgg tcgggtctcc gaggcggtgc aggcggcgcg
      481 cgatgcgctg caggcgctat atgaatcgat caagccgtac gaggagcgca gcgccgagat
      541 ggcctcgtgc gtggcgcgcg cggccgcgct ggtcgagcgc ctgcagacca tcctcgatga
      601 gcaggatcac gagcgcgtgc gctgggtgga ggcgctgggc cgcggcggca gcctgcacgc
      661 cacgccgatt ctcgtcgccg aaggtttccg ccgcatggcg gcgacctatc cgggcgcatg
      721 gatcttcacc tcggccacgc tggcggcgag cgaggacttc tcgcatttct ccggccagct
      781 cgggctggac caggcgcgcg agctcaagct cgacagcccc ttcgatttct ccacgcaggc
      841 gcggctgtac ctgcccaagg gcatgcccga cccgtccgat cccgcgtaca gcgatgccgt
      901 cgccgatgcc gcgctgccgc tgatccaggc cagcggcggc ggcgccttcg tgctgtgcac
      961 cagccaccgc gcgctgcgcc gcatcgccga gcggctcagg cccaagctca agcagaagct
     1021 ctacgtgcag ggcgacgaca gccgcgccgc gctgttgcag gccttcgccg cggacggcga
     1081 cggcgtgctc atcggcacca gcagtttctg ggagggcgtg gacgttaagg gccgcgcgct
     1141 gcggctggtc atcatcgaca agctgccgtt caccgcgcca ggcgacccgg tgttcgaggc
     1201 caagctcgcc gccatccgcg ccggcggcgg caacccgttc aacgatttcc aggtgccgca
     1261 ggccatcacc gcgctgcgcc agggcgtggg acgcctgatc cgcgacgccg cggatcgcgg
     1321 gctgctggcg ctgtgcgatc cgcgcctgcg cagcaagatg tacggccgcc gcatgctggc
     1381 cagcctgccg cagatgccgg tgctcagtga attggccgat gcgcaacaat ggctgcgctc
     1441 gctatgaaac tgctcgccat cgacaccgct accgaagcct gctccgtcgc gctgcacctc
     1501 gacggcgtgg tgagcgagcg tttcgtcaat gccgggcgca gccacaccga gctgctgctg
     1561 ccgatggtgc gcgggctgat ggccgacgcc ggcatcgcgt tcgggcaact cgacggcatc
     1621 gcctgcggca tcgggccggg cagcttcgcc ggcgtgcgca tcggcgtggg ctacgtgaag
     1681 ggcgcggcgc tggcggcgga tctgccggtg gtgggcatct cctcgctggc catgctggcg
     1741 cagggcgcga tcgcgcaggg acacgcgcgc gtgctgccgg tgatcgatgc caagaccaac
     1801 gagatctacg ccggactgta cgagcgcgat gcgcagggac tggcgcgcgc ggtgatgcct
     1861 gatctcgtct gcctgccggc cgaactgccg gcgacggagg gcgaatgcca cggctgcggc
     1921 agcggctggg gtacctacgg cgaaattttg cgcacgcgcg cgcgcgtcac ggcgagtgat
     1981 gcagcggcgc agccgcatgc gtccgccgcg ctcgcgctgg cgttgccgga gttcgcggcg
     2041 gggcgcggca tcggcgcgga cgagctggtg ccggcctacc tgcgcaataa agttgcactc
     2101 acgctgctcg aacaggctga cttgcgtcgc cggaataaag gataattccg cccggtccgc
     2161 gtcggccctt cgcgacaaag agttgtgaac cccgccaggc ccggaaggga gcaacggtag
     2221 cagcatattt gtgcgccgga gcaagctgac gcggaccact ttcgataacg ctgctgcgcg
     2281 atgggatatc gcctgagggc ggtgctcgga atcctcacat gttgatgtgt atgctccggc
     2341 tcctgtgctc cgtcctcagt cgatctgcca tctgttcaca acgcgttccc gaaggtggcc
     2401 gatgtgaatg aggagcatcg atgagctata tggcgctggc gcgcaaatgg cggccgcgga
     2461 agttcgagga cgtggcgggc caggatcacg tggtgaaggc gctgtcgcac gcgctggaca
     2521 ccgaccgcct gcatccggcc atcctgctca ccggcacgcg cggcgtgggc aagaccacgc
     2581 tggcgcgcat catcgccaag ggtctcaact gcgagaaggg cgtgtcggcg catccctgcg
     2641 gcgtgtgcgc ctcgtgccgg gagatcgacg agggccgcag cgtcgacatg ctggagatcg
     2701 acgccgcgtc gaacaccggc gtcgacaaca tccgtgaact gatcgagaac agccagtacg
     2761 cgccagccag gggccgctac aaggtttatc tcatcgacga agtgcacatg ctgtccaagg
     2821 gtgcgttcaa tgcgctgctg aagacgctgg aagagccgcc gccgcacatc aagttcatcc
     2881 tcgccaccac cgatccgcag aagctgccgg tgacggtgct gtcgcgctgc ctgcagttca
     2941 acctgaagcg cctgccgcag gtgctgatcc gcgcgcagat ggacaaggtg ctggccagcg
     3001 aaaacgtgca ggccgacgcc ggcgccgtgg ccgagctggc gcgcgccgcc gacggctcaa
     3061 tgcgcgacgg cctgtcgctg ctcgaccagg ccatcgccta cggcggcggc aaggtggagg
     3121 aggcggaggt gcgggccatg cttggtacca tcgagcacga ccatgtcacg cagctggtcg
     3181 aggcactggc cgcgggggac ggtccggtgc tgctgaaccg gatcgccgcc ctgaacgagt
     3241 t
//
LOCUS       DASZZC010000084         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_906671,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000084 DASZZC010000000
VERSION     DASZZC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906671"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VHE37_02050"
     CDS             complement(<1..804)
                     /locus_tag="VHE37_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT34339.1"
                     /translation="MHHHGSTQRALCLLTALCAAHVHAWADDAPADPPATTPATENVA
                     APADAGAPATTDAAPAAAADSAAAAATIPVEPLRTDASPQPAAPASTGDATQLEEVVV
                     TATKRAKNVREIPATIVGFSGEQLERDNVKGMEDLARLVPGVNYTMPSEGTPRITIRG
                     IAGQPATNATTGILYGDISFNDTYLPRVVPDPLPFDLQTVEILKGPQGTLFGAGALNG
                     AIRYVPEPAKPGVWETKYTAEYTRVHEGDRGAPVYGAVVNAPLYGGDTAA"
     gene            complement(1053..2606)
                     /locus_tag="VHE37_02055"
     CDS             complement(1053..2606)
                     /locus_tag="VHE37_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVT34340.1"
                     /translation="MSTTYLTQALHRAVRIHPQRTATICAARSHSYEQFTTRVARAAG
                     ALQKLGMKPGDRVGILALNSDRFLELYFAVWWGGGVVNPVNIRWSAREIAYSLDDCAT
                     QILAVDQAFAPLVPELSERSQALRTVLYIGDGAAASGMFEYEALIAEHAPVADAMRCN
                     GDLAGVFYTGGTTGFPKGVMLTHANLSYGASAALISGIAEEDDVGLHAAPMFHMADAM
                     FALALFLRGCTQAIVPMFNPETALAAIAGARVSVALLVPTMIQMLVDHPRLPEFDLAS
                     LRRVLYGASPISEALLARAMARLPNAGFIQCYGQTEMSPIVTVLSAADHLEGKRLRSA
                     GRPAVGVEVKIVDADDREVARGTVGEICARGPGVMAGYWNQPEQTAAAIRDGWMHTGD
                     GAYVDGDGYLYVVDRVKDMIVSGGENVYSAEVENAIAQHAAVAMCAVIGIPDEQWGEA
                     VHAVIVCRPGARVGADEIKAHCKALIATYKCPRSVELRDALPLSGAGKVLKNNLREPY
                     WAGRQRRVG"
BASE COUNT          394 a          975 c          975 g          456 t
ORIGIN      
        1 cgccgcggtg tcgccgccgt agagcggcgc gttgaccacc gcgccgtaca ccggcgcgcc
       61 gcggtcgcct tcgtgcaccc gcgtgtactc ggcggtgtac ttggtttccc acacgccggg
      121 cttggccggc tcgggcacgt agcggatggc gccgttgagc gcgccggcac cgaacagcgt
      181 gccctgcgga cccttgagga tctccacggt ctgcaggtcg aacggcagcg ggtcgggcac
      241 cacgcgcggc aggtaggtgt cgttgaacga gatgtcgccg tagaggatgc cggtggtggc
      301 gttggtcgcc ggctggccgg cgatgccgcg gatggtgatg cgcggcgtgc cttcgctggg
      361 catggtgtag ttgacgcccg gcaccagccg cgccaggtct tccatgccct tgacgttgtc
      421 gcgctccagc tgctcgccgg agaagccgac gatcgtcgcc ggaatctcgc gcacgttctt
      481 ggcgcgcttg gtggcggtga ccaccacttc ttccagctgg gtggcatcgc cggtgctggc
      541 gggcgctgcc ggttgcggcg atgcgtccgt gcgcaacggc tcgaccggga tggtcgccgc
      601 tgctgctgcg ctgtctgcag ctgcggctgg cgcagcgtcc gtcgtcgcgg gcgcaccggc
      661 atcggccggc gctgctacgt tttccgtagc aggcgtcgtg gcgggtgggt ccgccggcgc
      721 gtcatccgcc cacgcgtgca cgtgtgcggc gcagagcgcc gtcagcaggc acagcgcgcg
      781 ctgcgtggaa ccgtggtgat gcatcgctcc tcccgtcgca ttggccaaac ccgccgcgtg
      841 gcgtctgcat gcgccatcgc acccacctta ttgcaaagcg cggcggctgc accctcccgg
      901 atcggatggt gagcggtgcg ttctgggcgg cacgaagtcc gcaaaaaaat gcctcaccgc
      961 acctgcattc cgtgtgttat aaattattta ttaattaata aaatagacga taattaataa
     1021 catcgaaaac cgcgatgcgg tgatggcgtt tttcagccca cgcgccgctg gcggccggcc
     1081 cagtacggct cgcgcaggtt gttcttcagc accttgccgg cgcccgacag cggcagcgcg
     1141 tcgcgcagct cgacgctgcg cggacatttg taggtggcga tgagcgcctt gcagtgcgcc
     1201 ttgatctcgt cggcgccgac gcgcgcgccc ggcctgcaca cgatgaccgc gtgcaccgcc
     1261 tcgccccact gctcgtccgg gatgccgatc accgcgcaca tcgccaccgc cgcgtgctgc
     1321 gcgatggcgt tctccacctc ggccgagtac acgttctcgc cgccggacac gatcatgtcc
     1381 ttcacgcggt cgaccacgta gaggtagccg tcgccgtcga cataggcgcc gtcgccggtg
     1441 tgcatccagc cgtcgcggat ggccgcggcg gtctgctccg gctggttcca gtagccggcc
     1501 atcacgcccg ggccgcgcgc gcagatttcg cccaccgtgc cgcgcgccac ctcgcggtcg
     1561 tcggcgtcga cgatcttcac ctccacgccc accgcggggc ggccggccga gcgcaggcgc
     1621 ttgccttcaa ggtgatcggc cgccgacagc acggtgacga tcggcgacat ttccgtctgc
     1681 ccgtagcact ggatgaagcc ggcgttgggc aggcgggcca tggcgcgcgc cagcagcgct
     1741 tcgctgatcg gcgaggcgcc gtagagcacg cggcgcagcg aggcgagatc aaactccggc
     1801 aggcgcggat gatcgaccag catctggatc atggtcggca ccagcagcgc caccgacacg
     1861 cgtgcgccgg cgatggcggc cagcgcagtc tccgggttga acatcggcac gatcgcctgg
     1921 gtgcagccgc gcaggaacag cgccagcgcg aacatggcgt cggccatgtg gaacatcggc
     1981 gccgcgtgca ggccgacgtc atcctcttcc gcgatgccgc tgatcagcgc ggcgctggcg
     2041 ccgtacgaca ggttggcgtg cgtcagcatc acgcccttgg ggaatcccgt ggtgccgccg
     2101 gtgtagaaca cgccggccag gtcgccgttg cagcgcatgg cgtcggccac cggcgcgtgc
     2161 tcggcgatca gcgcctcgta ttcgaacatg ccgctggccg cggcgccgtc gccgatgtag
     2221 agcacggtgc gcagcgcctg cgagcgttcc gacagctccg gcaccagcgg cgcgaaggcc
     2281 tggtccaccg cgaggatttg cgtggcgcag tcgtccagcg agtaggcgat ctcgcgcgcg
     2341 ctccagcgga tgttcaccgg gttgaccacg ccaccgcccc accagacggc gaaatacagt
     2401 tcgaggaagc ggtcggagtt gagcgccagg atgccgacgc ggtcgcccgg cttcatgccc
     2461 agcttctgca gcgcgccggc cgcgcgcgcc acgcgcgtgg tgaactgctc gtagctgtgg
     2521 ctgcgcgcgg cgcagatggt ggcggtgcgc tgcgggtgga tgcgcaccgc gcggtgcagc
     2581 gcctgcgtga gataagtggt cgacatggca gttcctgttc tgacgccagc gtagggcctg
     2641 gccgcggcgg cttgccctcc cgcgtggaga gggggcgccg ccgctgcacc cgcgtagcgt
     2701 gccgccatgc ccgacgcgcc gccaccggcc acatcgctca caattccccg catgggcaac
     2761 accgagctgc cgctgatccg caccaagctg gcgccgccgc
//
LOCUS       DASZZC010000085         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_389057,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000085 DASZZC010000000
VERSION     DASZZC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389057"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VHE37_02060"
     CDS             <1..997
                     /locus_tag="VHE37_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVT34341.1"
                     /translation="VDGIDVVNDNTAGGVDAINAGLFPAGSGESHQFRVLDNGAATPR
                     DISMTSADITSTPVQYAHTISTPTGAVGYLLFNDHIATAESELVDAFNQLGTTGVQDL
                     VLDLRYNGGGYLDIASEVAYMIAGPASTAGVTFELQQFNDQYSTVNPVTGETITPVPF
                     HSTTQGFTLAEGQPLPTLNLSRVYVLTGSGTCSASEAIINGLRGAGINVIQIGNTTCG
                     KPYGFYPQDNCGTTYFSIEFRGVNAAGFGDYTDGFTPGAADSDATVRGCTVADDFTHL
                     LGDSSEARLAAALAYRSSGSCPGAAGAAKSAAVLHDAIIRKSPLRENRILRRSPR"
     gene            complement(1004..2395)
                     /gene="der"
                     /locus_tag="VHE37_02065"
     CDS             complement(1004..2395)
                     /gene="der"
                     /locus_tag="VHE37_02065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVT34342.1"
                     /translation="MQNGLPVLALVGRPNVGKSTLFNRLTGTRDALVADLPGLTRDRQ
                     YGYGQFEGRAFVVVDTGGLMPESSDPLAALAEEQAQLALAESDRVLFLVDSLAGRTGA
                     DQAIAQHLRKLGKPITLLVNKAEGQKRADAAADFYALGLGEPEPISADHGDGIAALLR
                     RLAQEFPQAEKPVDFGSIRVAVVGRPNVGKSTLVNRLLGEERMLAADQPGTTRDAIAV
                     PFEYEGHQYTLIDTAGIRRRGRVDEAIEKFSIVKTLAAIESAHVVIAVVDAQNDIGEQ
                     DARLIGLVAQRGRALVLAINKWDGLDDGGREAVRYQATLKLPFLDYAPLHFISARHGS
                     GVGVLMRDVYQAYESAMRDLPTPELTRALERMVAAHAPPAVMGRRIKLRYAHQGGQNP
                     PKIVIHGNQTDKLPNAYKRYLANEFRKWFDLKGVPLALSFKTGDNPYKHKKNVLTARQ
                     VKKKKRLIERTRR"
     gene            complement(2414..>3316)
                     /gene="bamB"
                     /locus_tag="VHE37_02070"
     CDS             complement(2414..>3316)
                     /gene="bamB"
                     /locus_tag="VHE37_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HVT34343.1"
                     /translation="LKPGNGDRIWRARTRARIVSGPGVFGSMVLVGTLDAEVIALNRA
                     DGKELWRSKVSSEVMAAPVGDGHVVVARTGDGQVHGLAADSGQALWHFDRTEPNLTLR
                     GLSDPLIVGNRVYVGMDNGRVVALSLDSGQLLWEQAVSVQTGRTELDRLTDIDGSMLP
                     SGNSVYAVSFGGELAALDDDNGEVTWRRSVKSYNGLAQSGDLVLTTDHNGVVWALDAA
                     SGAAVWKQEGLQYRKLSPPAAFAGYVVVGDYQGYLHWLDPKDGKVVGRSRAGSDPIRA
                     APIASDKLLYVLNTGGKLSAITVR"
BASE COUNT          518 a         1163 c         1077 g          558 t
ORIGIN      
        1 ggtggacggc atcgatgttg tcaacgacaa cacggccggc ggtgtcgatg cgatcaatgc
       61 cggcctgttt cccgccggca gcggcgaaag ccaccagttc cgcgtgctcg acaacggcgc
      121 ggcgacaccg cgcgacatct ccatgacgtc ggcggacatc acctccacac cggtgcagta
      181 cgcccacacc atttccacgc cgaccggcgc agtcggctac ttgttgttca atgatcacat
      241 cgccaccgcc gaatcggaac tggtcgatgc cttcaaccag ctgggtacca ccggggtgca
      301 ggacctggtg ctggacctgc gctacaacgg cggcggctac ctggacatcg ccagcgaggt
      361 ggcctacatg atcgcggggc cggccagtac tgcgggcgtc actttcgaac tgcagcagtt
      421 caacgatcaa tattccaccg tgaacccggt gaccggcgag accatcacac cggtgccgtt
      481 tcactcgacc acgcagggct tcacgctagc ggaaggtcag ccgctgccca cgctaaacct
      541 gtcgcgcgtc tacgtgctca ccggctcggg tacctgctcg gccagcgaag ccatcatcaa
      601 cggcttgcgg ggcgccggca tcaacgtgat ccagatcggc aacaccacct gcggcaagcc
      661 ttatggtttt tatcctcagg acaactgcgg cacgacctat ttcagcatcg agttccgcgg
      721 cgtgaatgcc gcaggcttcg gcgactacac cgacggattc acgccgggcg cggccgacag
      781 cgacgccacg gtgcgcggct gcacggtggc cgacgatttc actcaccttc tcggcgatag
      841 ttcggaagcg cggctggccg cggccctggc ctaccgcagc agcggcagct gtccgggcgc
      901 agcgggggcg gccaagagcg cggcggtgct gcacgacgcc atcatccgca agtcgccgct
      961 gcgcgaaaac cgcatcctgc gccgttcccc gcgctagcag cgttcagcgc cgggtccgct
     1021 cgatcaggcg tttcttcttc ttcacctggc gcgcggtgag cacgtttttc ttgtgcttgt
     1081 acgggttgtc gccggtcttg aacgacagcg ccagcggcac gcccttgagg tcgaaccact
     1141 tgcggaattc gttcgccagg tagcgcttgt aggcgttggg cagcttgtcg gtctggttgc
     1201 cgtggatgac gatcttcggc gggttctgcc caccctggtg cgcgtagcgc agcttgatgc
     1261 gccggcccat caccgccggc ggcgcgtgtg ccgccaccat gcgctcgagc gcgcgcgtga
     1321 gttccggcgt cggcaggtcg cgcatggccg attcgtaggc ctggtagaca tcgcgcatca
     1381 gcacgccgac ccccgagccg tgccgcgccg agatgaagtg cagcggcgca tagtccagga
     1441 acggcagctt gagcgtcgcc tgatagcgca cggcttcgcg cccaccgtcg tccaggccgt
     1501 cccacttgtt gatcgccagc accagcgcgc ggccgcgctg cgccaccagg ccgatcagcc
     1561 gcgcatcctg ctcgccgatg tcgttctgcg cgtcgaccac cgcgatcacc acgtgcgcgc
     1621 tctcgatcgc cgccagcgtc ttgacgatgg agaacttctc gatcgcctca tccaccctgc
     1681 cgcgccgacg gatgccggcg gtgtcgatca gcgtgtactg gtggccctcg tattcaaagg
     1741 gcacggcgat cgcatcgcgc gtggtgccgg gctggtcggc ggcgagcatg cgctcctcgc
     1801 cgagcaggcg attgaccagc gtggacttgc ccacgttcgg acgtccgacc acggcgacgc
     1861 ggatcgaccc gaaatcgacc ggcttttccg cctgcggaaa ttcctgcgcc agccggcgca
     1921 gcagcgcagc gatgccatca ccatgatcgg ccgagatggg ttcgggctca cccaggccga
     1981 gcgcgtagaa atcggccgcg gcatcggcgc gcttctgccc ttcggccttg ttcaccagca
     2041 gcgtgatggg tttgcccagc ttgcgcaggt gctgcgcaat ggcctgatcg gcgccggtgc
     2101 gtccggccag cgagtcgacc aggaacagca cgcggtcgga ctcggccagc gccagttgcg
     2161 cctgctcctc ggccagcgcg gcgagcggat cggacgattc cggcatcagc ccgccggtgt
     2221 cgaccaccac gaaagcacgt ccctcgaact ggccgtagcc gtactggcgg tcgcgggtca
     2281 ggcccggcag gtcggccacc agcgcgtcac gcgtgccggt cagccggttg aacagcgtgg
     2341 acttgcctac gttaggccgg ccgaccagcg ccagtacagg tagcccgttc tgcatgacgc
     2401 atagctctgc gggtcagcga accgtgatgg cggacagctt gccgccggtg ttgagcacgt
     2461 agagcagctt gtcgctggcg atcggtgcgg cgcgaatcgg atcgctgccg gcgcgcgagc
     2521 ggccgaccac cttgccgtcc ttggggtcca gccagtgcag gtagccctgg tagtcgccca
     2581 ccaccacgta gccggcgaat gcggccggcg gcgacagctt gcggtactgc agtccttcct
     2641 gcttccacac cgcggcgccg gaggcggcat cgagcgccca caccacgccg ttgtgatcgg
     2701 tggtcagcac caggtcgccg ctctgcgcca ggccgttgta gctcttgacc gagcgccgcc
     2761 aggtcacttc gccgttgtcg tcgtccagcg ccgccagttc gccgccgaag ctgaccgcgt
     2821 agacgctgtt gcccgacggc agcatcgacc cgtcgatgtc ggtcaggcga tcgagctcgg
     2881 tgcggccggt ctgcaccgat accgcctgtt cccacagcaa ctggccgctg tcgagcgaca
     2941 gcgccaccac gcggccgttg tccatgccca cgtacacgcg gttgccgacg atcagcggat
     3001 cggacaggcc gcgcagggtg agattgggct cggtgcggtc gaagtgccac agcgcctgcc
     3061 cgctgtcggc agccagtcca tgcacctggc cgtcgccggt gcgcgccacg acgacatggc
     3121 cgtcgcccac cggcgctgcc atcacctcgc tggaaacttt cgagcgccac agttccttgc
     3181 cgtcggcgcg gttgagcgcg atgacctcgg cgtcgagcgt gccgaccagc accatgctgc
     3241 cgaatacacc cgggccggag acgatgcgcg ccctggtgcg tgcgcgccag atgcggtcgc
     3301 cgttgcccgg cttgag
//
LOCUS       DASZZC010000086         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_389078,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000086 DASZZC010000000
VERSION     DASZZC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389078"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VHE37_02075"
     CDS             complement(<1..428)
                     /locus_tag="VHE37_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34344.1"
                     /translation="MSARPSGASAEILEAADLRFGQVGIANVRVRSIDPEALRAAIEV
                     RVHEAPALFERAPVVVDLSFLSPQPGDDAVRALLDAVRGAGMLPVGLAYGDDATDGLS
                     RRLGLPLIAKFRAQYERTERAASNDEAAPRVAASAQPAPSA"
     gene            complement(425..1039)
                     /locus_tag="VHE37_02080"
     CDS             complement(425..1039)
                     /locus_tag="VHE37_02080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT34345.1"
                     /translation="MTLTPNLTLRDVRASDLAAVMGLNNTAGSSILPMDAPQLNHLYQ
                     HADYFRVAEVDGHLAGFLIALQHTASHASSNFQWFRGHCERFVYIDRVVVGDLHRGHG
                     LGRTFYADVQSYAEVRSPNLACEVFLEPRDDVSVLFHGVFGFHEAGQQVMPETGRRVS
                     LLLKGLDCYPFVCDAYPDGLPALSWLTPRNLPDGEPQLRAAGRA"
     gene            1175..2377
                     /locus_tag="VHE37_02085"
     CDS             1175..2377
                     /locus_tag="VHE37_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVT34346.1"
                     /translation="MQRLSVSPVTLLRYAGFITYASAGVPLVRRNWIDAGAETQTAIH
                     ATRALHVQSHDMLLLWAVSYVVFGVVYWLLTRNLTSRRHLTMKLVGLLVLTATAIAIN
                     WFSVSGLGALLLAVATLVLPWLLPLPVAIVWLMLQYAAIIPVYAMFPNVTLDFAVIQS
                     GLYLGSSVIVFIASEVARQQVNAREEQRRLNSELRATRALLAESSRLTERMRIARELH
                     DLVGHHLTALSLNLEVAGHLVVNPDAADHVTRARNTAKQLLADVREVVSELRDDEGIQ
                     LTDALRDLTEGVPGLEVHLELPPRFAVADSRRAQVLLRCTQEIITNAVRHARARNLWL
                     RFEEGEGGKLLLKASDDGRGAIEFKPGNGLNGMRERLAEVGGKLAIETQRNQGFILEA
                     SLPMEHAG"
     gene            2456..3097
                     /locus_tag="VHE37_02090"
     CDS             2456..3097
                     /locus_tag="VHE37_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT34347.1"
                     /translation="MISVCLVDDQTLVRQGIRSLLELSPEIQVVAECSDGAAALQKIP
                     EVKPDVVLLDMRMPGKSGLEVLNEFSARGKVPPTIILTTFDDDQLVLAGLKAGARGYL
                     LKDVSLDQLVDAVKTVAGGGSLIAPVVTQRLLAGVNRINNQFSSLGQPDPLTDRETEI
                     LRLMSGGYSNKEIANSLKVAEGTVKNHVSNILSKLGVRDRTRAVLKALELGIV"
     gene            3505..>4760
                     /locus_tag="VHE37_02095"
     CDS             3505..>4760
                     /locus_tag="VHE37_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34348.1"
                     /translation="MTRVLEFVVALIIVFILAVIVGLFLPSHGHIQRSIEISHNPQHI
                     YDVLNNFRRFEDSTGGGLKALDPGVKFTLSGPAYGPGATISWQGDKAIGDGTLVNKSG
                     NIDITNKSTVTWDLTNDWHGTNKVFTLEVVPKQSQRVSTVTWSYDVDYGWNLIDRYSS
                     LWIHGDPSTVIQYGLGSLQNMLAGIANVDYSKVNPGLYRTDATPVLLVSTKSPRTVDD
                     IDTAKAAAMKEIQAAMAKLGVKAAGPTTTIRTEWGDTTFTFDLAVPINSTTLTVNGKT
                     VDLTKLPPPPNPNAAPPASATPASSSSAASASSAGAPASASSAGGAAENGPAPGSLDK
                     QNRVVIDANVRAAMMPATEVLAATWTGESGVEFMVKALEAYAGTHGYKFNEAVAPAFN
                     QLASLPSVTDDDQIYRVFLPVQDAPA"
BASE COUNT          814 a         1582 c         1571 g          793 t
ORIGIN      
        1 gccgacggag ccggttgggc gctcgccgcg acgcgcggcg cggcttcatc gttcgaggcc
       61 gcgcgctcgg tgcgttcgta ttgcgcgcgg aatttcgcga tcagcggcag gccgaggcgc
      121 cgtgacagtc catcggtcgc atcgtcgcca taggcaaggc ccaccggcag catgcccgcg
      181 ccgcgcacgg catcgagcag cgcgcgcacg gcatcgtcgc ccggttgcgg cgacaggaac
      241 gacaaatcca ccaccaccgg cgcgcgttcg aacaacgcgg gtgcttcgtg cacgcgcact
      301 tcgatggcgg cgcgcaatgc ctcgggatcg atgctgcgca cgcgcacgtt ggcgatgccg
      361 acctggccga aacgcaggtc ggcggcttcc aggatttcgg cgctggcgcc ggacggacgc
      421 gcgctcacgc ccgccccgcc gcgcgcagct gcggttcgcc atccggcagg ttgcgtggcg
      481 tcagccacga caatgcaggc aggccgtcgg ggtaggcatc gcacacgaat ggatagcagt
      541 cgagtccctt gagcagcagg ctgacgcggc ggccggtttc cggcatcacc tgctggccgg
      601 cttcgtggaa accgaacacg ccgtggaaca gcaccgaaac gtcgtcgcgc ggttccagga
      661 acacttcgca ggcgagattg ggcgaacgca cttccgcata gctttgcacg tcggcgtaga
      721 acgtgcggcc cagtccatgg ccacggtgca ggtcgccgac gaccacgcgg tcgatataca
      781 cgaagcgctc gcagtggccg cggaaccatt ggaaattgga actggcgtgc gaagctgtgt
      841 gctgcagcgc gatcaggaat ccggccaggt ggccgtccac ttcggcgacg cggaaataat
      901 cggcatgctg gtacagatgg ttcagttgcg gcgcatccat cggcaggatg gaggagccgg
      961 cggtgttgtt gagtcccatc acggcggcaa gatcgctcgc acgcacgtcg cgcagggtca
     1021 ggttcggcgt cagcgtcatg tgtagcttgg tttccggact gcggctggcc attgcggcca
     1081 ccatcatcgc accattattg catgccatcc gctgctgtcg cgaagccggc cctgaggcgc
     1141 ggcccgcgcg tcccggctcg gttatcatcg gcgcgtgcaa cgcctctccg tcagtcccgt
     1201 caccctgctt cgctacgcgg ggttcatcac gtacgccagt gcgggcgtgc cgctggtgcg
     1261 gcgcaactgg atcgatgccg gcgccgagac gcagacggcc atccatgcga cgcgcgccct
     1321 gcacgtgcag agccacgaca tgctgttgct gtgggccgtc agttacgtcg tgttcggcgt
     1381 ggtctactgg ttgttgacgc gcaacctcac gtcgcggcgc cacctgacca tgaagctggt
     1441 gggattgctg gtgctgacgg ccacggcgat cgcgatcaac tggttcagcg tcagcgggct
     1501 gggcgccttg ctgttggcgg tggccacgct ggtgctgccg tggctgctgc cgctgccggt
     1561 ggcgatcgta tggctgatgc tgcagtacgc ggcgatcatt cccgtctacg cgatgttccc
     1621 gaacgtcacg ctggatttcg ccgtcatcca gtccgggctc tacctcggtt catcggtgat
     1681 cgttttcatc gcttccgaag tcgcgcgcca gcaggtcaac gcgcgcgagg agcagcggcg
     1741 gctgaattcc gaactgcgcg ccacgcgcgc gttgctggcg gaatccagcc ggctgaccga
     1801 gcgcatgcgc atcgcgcgcg agttgcacga tctggtcggc caccacctca ctgcgctcag
     1861 cctcaacctc gaagtggccg ggcacctggt cgtgaacccg gacgcggccg accacgtcac
     1921 ccgtgcccgc aatacggcca agcaattgct ggccgacgtg cgcgaagtcg tcagcgaact
     1981 gcgcgacgac gaaggcatcc agttgaccga tgcgctgcgc gatctcaccg agggcgtgcc
     2041 cggcctggaa gtgcatctcg aattgccgcc gcgtttcgcg gtggccgatt cgcgccgcgc
     2101 gcaggtgctg ctgcgctgca cgcaggagat catcaccaac gcggtacggc acgcgcgcgc
     2161 acgcaacctg tggctgcgtt tcgaggaagg cgagggcggc aagctgctgt tgaaagccag
     2221 cgacgacggc cgtggcgcga tcgagttcaa gcccggcaac ggcttgaacg gcatgcgcga
     2281 acggctggcc gaagtgggcg gcaagctggc catcgaaacg cagcgcaacc agggtttcat
     2341 cctggaagcg tcgttgccga tggagcacgc gggctagcat catgcagatt cgcggcgcac
     2401 atgtgccgca ggggtggcgc gcggtgggcg ccgatttttc gagagggcag aaacgatgat
     2461 ttccgtgtgt cttgtggacg accagacgct ggtgcgtcag ggcatacgct cgctgctgga
     2521 gctgtcgccc gaaatccagg tggtggccga gtgcagcgac ggtgccgcgg cgttgcagaa
     2581 gattcccgag gtcaagcccg acgtggtcct gctcgacatg cgcatgcccg gcaagagcgg
     2641 cctcgaagtg ctcaacgaat tttccgcgcg cggcaaggtg cccccgacca tcatcctgac
     2701 caccttcgac gacgaccagc tggtgctggc ggggctgaag gccggcgcgc gcggttacct
     2761 gctgaaggac gtgtcgctgg atcaattggt ggacgcggtg aagaccgtcg ccggcggcgg
     2821 ctcgctgatc gcgccggtgg tgacgcagcg cctgctggcc ggcgtcaacc gcatcaacaa
     2881 ccagttctca agcctcggcc agcccgatcc gctgaccgat cgcgaaaccg aaatcctgcg
     2941 cctgatgtcc ggcggctaca gcaacaagga aatcgccaat tcgctgaagg tggcggaggg
     3001 cacggtgaag aaccatgtct ccaacattct ttccaagctg ggcgtgcgcg accgcacgcg
     3061 cgcggtgctc aaggcgctgg agctgggcat cgtctaggaa tcgccgcgtg gcctactgcg
     3121 ctcgtcggct cgggcgccgg cggtgctcgg catcctcatg tactgacgtg tacactccgg
     3181 tgcctgcgct gactcgccac atccctgtgg ctcgctcttc gagccgcctg cggcgttcaa
     3241 atcggcagtc ctgccgattt agtcggcgac gcccaagccg ccttcgctcg cgacggccac
     3301 acgacgattc cggccagata gcttcttgct gcgcagcccg ccgcggccgt tcagggagcc
     3361 gctcgcgcgg gccacttccg gggcatggcg cgaacgcggc gaagccgcta acatccgcga
     3421 ttcacgtttc ccattgacgt ggcacctgcc ggttccagcg gcaggcgccg accaagtgaa
     3481 caaagccgtc cggagattcc tggcatgacc cgtgtgctcg aattcgtcgt agccctgatc
     3541 atcgtcttca tcctggccgt gatcgtggga ctgttcctgc ccagccacgg gcacatccag
     3601 cgctcgatcg agatcagcca caatccgcag cacatctatg acgtcctgaa caacttcagg
     3661 cgtttcgagg actccacggg cgggggcctg aaggcgctcg acccgggcgt caagttcacg
     3721 ctgtccggcc cggcctacgg ccccggcgcg accatcagct ggcagggtga caaggccatc
     3781 ggtgacggca cgctggtcaa caagtccggc aacatcgaca tcaccaacaa gagcaccgtc
     3841 acctgggatc tgaccaacga ctggcacggc accaacaagg tgttcacgct ggaagtcgtg
     3901 ccgaagcaga gccagcgcgt cagcacggtg acctggtcgt acgacgtgga ctacggctgg
     3961 aacctcatcg accgctattc gtcgttgtgg atccatggcg atccctccac ggtgatccag
     4021 tacggcctcg gttcgctgca gaacatgctg gccggcatcg ccaacgtcga ttacagcaag
     4081 gtcaatcccg gcctgtaccg caccgatgcc acgccggtgc tgctggtttc caccaagtcg
     4141 ccgcgcacgg tggacgacat cgacacggcc aaggccgcgg cgatgaagga aatccaggcg
     4201 gcgatggcca agctgggcgt gaaggcggcg ggtccgacca ccaccatccg caccgagtgg
     4261 ggcgacacca ctttcacctt cgacctggcg gtgccgatca acagcaccac cctgaccgtc
     4321 aatggcaaga ccgtcgattt gaccaagctg ccgccgccgc cgaatccgaa cgctgcgccg
     4381 cccgcttcgg cgaccccggc gtcgtcttcc agcgcggctt cggcgtccag cgccggcgcg
     4441 ccggcctccg cttcgtccgc ggggggtgcg gccgagaacg gccctgcgcc gggcagcctc
     4501 gacaagcaga accgcgtggt catcgatgcc aacgttcgcg cagcgatgat gcccgccacc
     4561 gaagtgctgg cggccacgtg gaccggcgag agcggcgtgg agttcatggt caaggcgctc
     4621 gaggcctacg ccggcacgca tggttacaag ttcaacgaag cagttgcgcc agccttcaac
     4681 cagcttgcgt cgctgccgag cgtgaccgac gacgaccaga tctaccgcgt gttcctgccc
     4741 gtgcaggatg cgccggcgca
//
LOCUS       DASZZC010000087         5583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_518651,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000087 DASZZC010000000
VERSION     DASZZC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518651"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..861
                     /locus_tag="VHE37_02100"
     CDS             124..861
                     /locus_tag="VHE37_02100"
                     /inference="COORDINATES: protein motif:HMM:NF005489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT34349.1"
                     /translation="MKRVLIIGATSAIAEAVARLCAARGDALLLAARDARKLQTIAAD
                     LAVRGAAGVHTLPFDALAGDALAALVEQAARTLGGIDLALIAHGSLPEQAACEQSVEL
                     TRREFELNGLSVIVLCTLLAQKLEAQRRGTLAVISSVAGDRGRASNYVYGAAKAAVSA
                     FLSGLRQRLRPAGVQVLTIKPGFVDTPMTAQFAKNALWASPPLVARDILRAVESGQPQ
                     LYTPWFWWWIMAIIRAIPERLFARIKL"
     gene            complement(1007..4117)
                     /locus_tag="VHE37_02105"
     CDS             complement(1007..4117)
                     /locus_tag="VHE37_02105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT34350.1"
                     /translation="MEWYRLWNDPDRPRRWLLLPATLAAVVLCLVSLGNLRAAHALQS
                     HIDRDYAEVYVGRALREPITSPREAALRDAVNRLVRREDLGVLFLTVRDAAGVLRASA
                     GRYENLDVGWLPRSADHLLRDWLYERDAQSGHGLILADDGKAAGSFDYTIANGVQTAV
                     HDAAVSRLLWSGWLGVAAGLVMLAWLAFALRRQLTEPPPASALLERMRMPPRTPGEAV
                     PGEDADLEGEAALRSRMGLAMDQLRYGVLVADRDARVRHLNKVAEQLTGWPLGDARTR
                     LVYSVFHAVDEHNQPLLSAAEACLKEGREVAVQECALRGRDGSLLPIEMMAGITRDRE
                     GVVDGTVMLFRDIGPRHRRMEELKRQSRLSQGVIDHLEEGLLTTDHAGVVHFANARAL
                     RMFGYAREELEGVTITKLMPVPFLNTPSIKLMDYAGARNARKLPKVVGWRKDATTFPA
                     ELQVQPMSVGDDNGLIVIVRDISERLRGENLATRLGRLLDSALEEVYIFDAQSLYFLE
                     VNRGARKNLGYRQEQLARMTPLTISSHLEEAEFLGYLSKLRGGDIEHLTYRAAHRRAD
                     GTEYPVEVRLNFSREEEPPVFMAIAVDISERVAAEEKLKHLAHHDALTGLPSRSVLYD
                     RLNQSLLAASRSNRMVAVYFVDLDLFKNINDSYGHEVGDAALKCVSERLKAQLRAADT
                     VARLAGDEFVIIATGIRGTDDAEALAQKVVESFRHRLDIPGHEITMSVSVGVAIYPLD
                     EGDADALLRHADAAMYMAKQAGRGCYRLFSSDIDPLRRRRLDLEREIHTAVALNQFHI
                     TLAPVISQRDAVAAALVDFYWEHPRSERVGSRESLRAATRAGLLADLELWLICNSCAE
                     LHAMRQAGAPMLPAIVSISGWQLRESNFGEHVLTLMERFEVQPGMLILALTQDGMTEA
                     EAMPERLVPLVNRGVRFALRDFSGGFEALNRSGAVPFDLILMPEETAARAVAESEVAR
                     RLRAVLESAAATARKVVVTSVASTRVRTHLEGLGNTYAAGPLYRADLGGRDGAQWLAA
                     QRAEPL"
     gene            complement(4190..>5583)
                     /locus_tag="VHE37_02110"
     CDS             complement(4190..>5583)
                     /locus_tag="VHE37_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34351.1"
                     /translation="FDPTPADYSRAVQVTLTRPMPDRLVPFTVANAADGPQTLSARAA
                     AYATPSATVMMGTQTLALSPNAGVLNNLLSGLLGGPVSLNLLTYQALFSANVPVLSVL
                     DNLDIQTPEQLESTTVGERDLLNALASALSQAGQAAAASAASTLAATASNTRTLPLMQ
                     ALGVGDVTSDAMVNAGRTILGVSQAVLGGNLLNLSLSGGNVQLGITQAALPTLLLPGG
                     IDLFPNNYASNTQLALLINALPLNIPLLGMNGNVSLFAQVGQGTAEVDHLVCARRGVP
                     NDLAYVRARTSVVRLGIGKFDNINSPNPTPQAATILNTNGTINLAGIPLPVPVKINIS
                     AYFELPLDLGSGEQMLPAMKEGDTYHMGTPRVSQITGNLTPSINTVNVSIQTNGPLSG
                     VLALLSGTINSLLATLQTTVQAALQSQLTSLLSSVADATLLPILDSAGVTIGGADVTV
                     TNIDAPQPYLFTH"
BASE COUNT          813 a         1889 c         1983 g          898 t
ORIGIN      
        1 caaggatgcg cgcatgagcg cggcttcgtt ccagcaatac ttcccgcgct ggcgcgaatt
       61 cgccgcgcag gtcgatccaa aattctcctc gtcgttctgg cggcgggtca cgcagggagc
      121 ctcatgaaac gcgtattgat catcggcgcc acatcggcca tcgccgaagc ggtggcgcgg
      181 ctgtgcgcgg cgcgcggcga tgcgctgctg ctggccgcgc gcgacgcgcg caagctgcag
      241 acgatcgccg ccgaccttgc cgtgcgcggc gcggccggcg tgcacacgct gccgttcgat
      301 gcgctggccg gcgatgcgct ggccgcgctg gtcgagcagg ccgcgcgcac gctgggcgga
      361 atcgacctgg cgctgatcgc gcacggcagc ctgcccgagc aggcggcgtg cgagcagtcg
      421 gtcgagctca cgcggcgcga gttcgagctg aacgggctgt cggtgatcgt gctgtgcacg
      481 ctgctggcgc agaagctgga agcgcagcgg cgcggcacgc tggcggtgat ctcgtcggtg
      541 gccggcgatc gcggacgcgc gtcgaactac gtgtacggcg cggccaaggc ggcggtcagc
      601 gcgttcctgt ccggcctgcg ccagcggctg cggccggccg gcgtgcaggt gctgaccatc
      661 aagcccggtt tcgtcgacac gccgatgacc gcgcagtttg ccaagaacgc gctgtgggcc
      721 tcgccgccgc tggtggcgcg cgacatcctg cgcgcggtgg agtcgggcca gccgcagctg
      781 tacacgccgt ggttctggtg gtggatcatg gcgatcatcc gcgccatccc ggaacgcctg
      841 ttcgcgcgca tcaagctgtg aaccggcgcg gccggcgagc ggaatgaggc cggaaacacg
      901 cccactaccc cgtccgtttc ggtgttatta attgtcttat tatttttact ttaaacaata
      961 attaataaca tcgccaatgc cggtgcggtg cccggttttt ttgcgcctac agcggctccg
     1021 cgcgctgcgc cgccagccac tgcgcgccat cgcgcccgcc caggtcggcg cggtacagcg
     1081 ggcctgcggc ataggtgttg cccagccctt ccaggtgtgt gcgcacgcgc gtgctggcca
     1141 cgctggtcac caccaccttg cgcgcggtgg cggcggcgct ctccagcacc gcgcgcaggc
     1201 gccgcgccac ttccgattcc gccaccgccc gcgcggcggt ttcttccggc atcaggatca
     1261 ggtcgaacgg caccgcgccg ctgcggttca gcgcctcgaa gccgccggag aaatcgcgca
     1321 gcgcaaagcg cacgccgcgg ttgaccagcg gcaccagccg ctccggcatg gcctcggcct
     1381 cggtcatgcc gtcctgggtc agcgccagga tcagcatgcc gggctgcacc tcgaagcgct
     1441 ccatcagcgt cagcacgtgc tcgccgaaat tgctctcgcg cagctgccag ccggagatgc
     1501 tgacgatggc cggcagcatc ggtgcgcccg cctggcgcat ggcgtgcagc tcggcgcagg
     1561 agttgcagat cagccacagc tcgagatcgg ccagcagccc ggcgcgcgtc gccgcgcgca
     1621 ggctctcgcg cgaacccacg cgctcggagc gcggatgctc ccagtagaaa tcgaccagcg
     1681 cggcggccac cgcgtcgcgc tggctgatca ccggcgccag cgtgatgtgg aactggttga
     1741 gcgccaccgc ggtgtggatc tcgcgctcca ggtccagccg gcgccggcgc agcgggtcga
     1801 tgtcggagga gaacagccgg tagcagccgc gtccggcctg cttggccatg tacatcgccg
     1861 cgtcggcatg gcgcagcagc gcgtcggcat cgccctcgtc cagcgggtag atggccacgc
     1921 ccacgctcac gctcatggtg atctcgtggc cggggatgtc cagccggtgg cggaagctct
     1981 ccaccacctt ctgcgccagc gcctcggcgt cgtcggtacc gcggatgccg gtggcgatga
     2041 tgacgaactc gtcgccggcc aggcgcgcca ccgtgtcggc cgcgcgcagc tgcgccttca
     2101 gccgctcgct cacgcacttg agcgcggcgt cgccaacctc gtggccgtag ctgtcgttga
     2161 tgttcttgaa caggtcgagg tcgacgaagt acaccgccac catgcggttg ctgcgcgagg
     2221 ccgccagcag cgactggttg aggcggtcgt acagcaccga gcggctgggc aggccggtga
     2281 gcgcgtcgtg atgcgccaga tgcttgagct tctcctcggc ggccacgcgc tcgctgatgt
     2341 cgaccgcgat ggccatgaac accggcggct cttcctcgcg cgagaaattc agccgcacct
     2401 ccaccgggta ctcggtgccg tcggcgcggc ggtgggccgc gcggtaggtc aggtgctcga
     2461 tgtcgccgcc gcgcagcttc gacaggtagc cgagaaactc cgcttcttcc aggtggctgc
     2521 tgatggtcag cggcgtcatg cgcgccagct gctcctggcg gtagcccagg ttcttgcgcg
     2581 caccgcggtt cacctcgagg aagtacagcg actgcgcgtc gaagatgtag acctcttcca
     2641 gcgcgctgtc gagcaggcgg ccgagacgcg tggccaggtt ctcgccgcgc aggcgctcgg
     2701 agatgtcgcg cacgatgacg atcagcccgt tgtcgtcacc cacgctcatc ggctgcacct
     2761 gcagctcggc cgggaaagtg gtggcgtcct tgcgccagcc caccaccttg ggcagcttgc
     2821 gcgcgttgcg cgcgccggcg tagtccatca atttgatcga cggcgtgttg aggaacggca
     2881 ccggcatcag cttggtgatg gtgacgcctt ccagctcttc gcgcgcgtag ccgaacatgc
     2941 gcagcgcgcg cgcattggcg aagtgcacca cgccggcatg gtcagtggtg agcaggcctt
     3001 cctccaggtg atcgatcacg ccctgcgaca ggcgcgactg gcgcttgagc tcctccatgc
     3061 gccggtggcg cgggccgatg tcgcggaaca gcatgacggt accgtcgacc acgccttcgc
     3121 gatcgcgggt gatgccggcc atcatctcga tgggcagcag cgagccgtcg cgcccgcgca
     3181 gcgcgcattc ctgcaccgcc acctcgcggc cttccttgag acaggcttcg gccgccgaca
     3241 gcagcggctg gttgtgctcg tccaccgcgt ggaacaccga gtagaccagg cgcgtgcgcg
     3301 catcgcccag cggccagccg gtgagctgct cggccacctt gttgagatga cgcacgcggg
     3361 cgtcgcgatc cgccaccagc acgccgtagc gcagctggtc cattgccagg cccatgcgcg
     3421 agcgcagcgc ggcctcgcct tccaggtcgg catcttcgcc gggtacagcc tcgcccggcg
     3481 tgcgcggcgg catgcgcatg cgctcgagca gcgcgctggc cggcggtggc tcggtcagct
     3541 ggcggcgcag ggcgaaagcc agccaggcca gcatcaccag accggcggcg acgccgagcc
     3601 agcccgacca cagcagccgc gacaccgccg cgtcatgcac cgcggtctgc acgccgttgg
     3661 cgatggtgta gtcgaagctg ccggcggcct tgccgtcgtc ggccaggatc aggccgtggc
     3721 cggattgggc gtcgcgttcg tacagccagt cgcgcagcag atggtcggcc gatcgcggca
     3781 gccagccgac atcgaggttt tcataacggc cggcgctggc gcgcagcacg ccggcggcgt
     3841 cgcgcacggt gaggaacagc acgcccaggt cctcgcgccg caccagccgg ttgacggcgt
     3901 cgcgcagcgc ggcttcacgc gggctggtaa tgggctcgcg cagcgcgcgg ccgacataga
     3961 cctcggcata gtcgcggtcg atatgcgatt gcagcgcgtg tgcggcgcgc aggttgccca
     4021 gtgaaaccag gcacagcacc acggcagcaa gggtcgccgg cagcagcagc cagcggcgcg
     4081 gccgatcggg gtcgttccac aggcggtacc actccatgac gctagtttgc catggcgccg
     4141 tgctggcgca tgcgcgtagg ccgttccatg gcgggcatgg ccggccggct cagtgcgtga
     4201 acaggtacgg ctgcggtgcg tcgatgttgg tcaccgtgac atcggccccg ccgatggtga
     4261 cgccggcgct gtcgaggatc ggcagcaggg tggcgtcggc cacactggac agcagtgagg
     4321 tcagttgcga ttgcagcgcc gcctgcaccg tggtctgcaa ggtggccagc agcgaattga
     4381 tggtgcccga cagcagggcc agcacgccgc tgagcggccc gttggtctgg atgctgacgt
     4441 tgaccgtgtt gatcgacggc gtcaggttgc cggtgatctg cgaaacccgc ggcgtgccca
     4501 tgtgataggt atcgccttcc ttcatcgccg gcagcatctg ctcgccgctg cccaggtcca
     4561 gcggcagctc gaaatacgcg ctgatgttga tcttcaccgg caccggcagc gggatgcccg
     4621 caagattgat cgtgccgttg gtattgagga tggtggcggc ctgcggcgtg ggattgggcg
     4681 agttgatgtt gtcgaacttg ccgatgccca ggcgcacgac actggttcga gcgcgtacgt
     4741 aggcgaggtc attgggcacg ccgcggcggg cgcagaccag gtgatcgacc tcggctgtgc
     4801 cctggcccac ctgcgcaaac aggctgacgt tgccgttcat gcccagcagc gggatgttga
     4861 gcggcagcgc gttgatcagc agcgccagct gggtgttgga ggcgtagttg ttggggaaga
     4921 ggtcgatgcc gcccggcagc agcagcgtgg gcagcgccgc ctgggtgatg cccagctgca
     4981 cgttgccgcc gctgaggctg aggttgagca ggttgccgcc cagcaccgcc tggctcacgc
     5041 ccaggatggt gcggccggcg ttgaccatgg cgtcgctggt cacgtcgccc acgcccagcg
     5101 cctgcatcag cggcagcgtg cgcgtgttgc tggcggtggc agcgagcgtc gatgcagcgc
     5161 tcgcagctgc ggcctggccg gcctgcgaca gcgcgctggc cagcgcgttg agcaggtcac
     5221 gctcgcccac cgtggtcgat tccagctgct ccggcgtctg gatgtcgagg ttgtcgagca
     5281 ccgacagcac cggcacgttg gccgagaaca gcgcctggta ggtgagcagg ttgagcgaca
     5341 ccggtccgcc gagcaggccc gacagcaggt tgttgagcac gccggcgttg ggcgacagcg
     5401 ccagtgtctg cgtgcccatc atcaccgtgg cgctgggcgt ggcataggcc gcggcgcgcg
     5461 cactgagcgt ctgcgggcca tcggccgcgt tggccacggt gaacggcacc aggcggtcgg
     5521 gcatcggccg cgtcagcgtc acctgcaccg cgcggctgta gtccgccggc gtcggatcga
     5581 agt
//
LOCUS       DASZZC010000088         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_775096,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000088 DASZZC010000000
VERSION     DASZZC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775096"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VHE37_02115"
     CDS             <1..366
                     /locus_tag="VHE37_02115"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HVT34352.1"
                     /translation="LPLEPYGANYNPSTGAGSLAVELVGTSVTSPGFDDLNGYSADGL
                     QSVNPHLKYVELTQRGYLLLDINSQRVSGEWWYVNTIDTRDDTQSFATALQTSDQANH
                     LEPGAQSPPKPHAPPLAPA"
     gene            complement(377..>1768)
                     /locus_tag="VHE37_02120"
     CDS             complement(377..>1768)
                     /locus_tag="VHE37_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT34353.1"
                     /translation="EVLSRLTADTTLVQTVVGSSVSMGLRSFFQFIGGLVMLAVTSLK
                     LFSITVVALLLVVVPLIAAGRGVRRLSRESQDRIADTSAVAGEILNAMPTVQAYTSEA
                     FEAARYERSVDGSFVSAIRRTRLRAYMTAGAITGVFGAIVFVLWLGARSVADGSMSAG
                     QLTSFILYAVFTAGGIGVVAEVWGDIMRAAGATERLMELLAARPVIRAPAQPVALPER
                     AQASVNFDSVVFHYPSRPQTAALDGFTLEINAGETVALVGPSGAGKTTVFQLLLRFYD
                     VGGGAIRVNGVDLRQLDPADLRGHIGIVPQDAVIFSADAMENIRYGNAQASDEQVMEA
                     ARMALADEFIARLPQGYKTFLGERGLRLSGGQRQRIAIARAILRNPPLLLLDEATSAL
                     DAASEALVQRGLEAAMKNRTTLIIAHRLSTVRQASRIVVLEHGRIVEVGTPAELMQQG
                     GLYSRLANLQIVA"
BASE COUNT          287 a          630 c          600 g          251 t
ORIGIN      
        1 ctgccgctgg aaccttacgg cgccaactac aacccgtcga ccggcgccgg ctcgctcgcg
       61 gtggaactgg tgggcacatc ggtcacctcg cccggcttcg acgacctgaa cggctactcg
      121 gcggatggct tgcagtcggt caacccgcat ctgaaatacg tcgagctgac gcagcgcggc
      181 tacctgctgc tggacatcaa cagccagcgc gtgtcgggcg agtggtggta cgtgaacacc
      241 atcgacacgc gcgacgacac gcagtcattc gccaccgcgc tgcagacgtc cgaccaggcc
      301 aatcacctgg agccgggcgc gcagagcccg cccaagccgc acgcgccgcc gctggcgccg
      361 gcttgaagga aaaagctcag gccacgatct gcagattcgc cagccgcgag tagagcccgc
      421 cctgctgcat cagttccgcc ggcgtgccga cttccacgat gcgcccgtgt tccagcacca
      481 cgatgcggct ggcctggcgc accgtcgaga ggcggtgcgc gatgatcagc gtggtgcggt
      541 tcttcatcgc cgcttccagt ccgcgctgca ccagcgcctc gctcgccgca tcgagcgcgc
      601 tggtggcttc gtcgagcagc agcagcggcg gattgcgcag gatggcgcgc gcaatggcga
      661 tgcgctggcg ctggccgccg gacaggcgca gcccgcgctc gcccaggaag gtcttgtaac
      721 cctgcggcag gcgcgcgatg aattcatcgg ccagtgccat gcgcgcggcc tccatcacct
      781 gctcgtcgct ggcctgcgcg ttgccgtagc ggatgttctc catggcatcg gcggagaaga
      841 tcaccgcgtc ctgcggcacg atgccgatgt gcccgcgcag gtcggccgga tcgagctggc
      901 gcaggtccac gccgttgacg cggatggcgc cgccgcccac gtcgtagaaa cgcagcagca
      961 gctggaacac ggtggtcttg ccggcgccgc tggggccgac cagcgccacc gtttcgccgg
     1021 cgttgatctc cagggtgaag ccgtcgagcg ccgcggtctg cgggcgcgac gggtagtgga
     1081 acaccacgct gtcgaaattc acgctggcct gcgcgcgctc gggcagcgcc accggctgcg
     1141 ccggcgcgcg gatcaccggc cgtgccgcca gcagttccat cagccgctcg gtggcgccgg
     1201 cggcgcgcat gatgtcgccc cacacttccg ccaccacgcc gatgccgccg gcggtgaaca
     1261 ctgcgtacag gatgaacgag gtgagctggc ccgcgctcat gctgccgtcg gccaccgagc
     1321 gtgcgcccag ccacagcacg aaaacgatgg cgccgaacac gccggtgatg gcacccgcgg
     1381 tcatgtacgc gcgcaggcgc gtgcggcgga tggcggagac gaagctgccg tccaccgagc
     1441 gctcgtagcg cgcggcctcg aaggcttcgc tggtgtacgc ctgcaccgtc ggcatggcgt
     1501 tgaggatttc gccggccacc gccgaggtgt cggcgatgcg gtcctgcgat tcgcgcgaca
     1561 ggcgccgcac gccgcggccg gccgcgatca gtggcaccac caccagcagc agcgcaacca
     1621 cggtgatcga gaacagcttc aggctggtga ccgccagcat caccagcccg ccgatgaact
     1681 ggaagaagct gcgcagcccc atggacaccg atgagccgac caccgtctgc accagcgtgg
     1741 tgtcggcggt gaggcgcgac agcacttc
//
LOCUS       DASZZC010000089         7736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1035104,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000089 DASZZC010000000
VERSION     DASZZC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035104"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VHE37_02125"
     CDS             <1..463
                     /locus_tag="VHE37_02125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT34354.1"
                     /translation="DIDQFKLINDTHGHLFGDEVIRKVGQAISAHYALRPNDLKARFG
                     GEEFVLLWYQVSGAHFGQLVERLLSAIRQLELPGSDTELVRITASAGALWLVPDSSRP
                     AEAILNQADQLLYQAKAGGRDRMELVILGDPAATDVEPGDQPYTARPAAGL"
     gene            454..527
                     /locus_tag="VHE37_02130"
     tRNA            454..527
                     /locus_tag="VHE37_02130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:488..490,aa:Ile,seq:cat)
     gene            complement(531..1388)
                     /locus_tag="VHE37_02135"
     CDS             complement(531..1388)
                     /locus_tag="VHE37_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT34355.1"
                     /translation="MTTLLRYCLVAIGWLPLPVLHGAGAALGGLLWLLPTRQKRITLR
                     HVQLAFPDLDAAARRHIARTSFLESGKAIAEAPAIWFGPAFRLRRWLRDDQAQRALLG
                     YMRNGAAIALCPHLGSWELAGMFCAAHAGITSLYKPQKGAIDALILQGRQRLGARLVP
                     TEGGGVRALLQALKDRQLIGILPDHDPQEGSGEFAPLFGAIAHSPSLVTKLAARTGAP
                     VFFCLAERRTWGRGFRFHIVAAPADIGESVEALNRGVEQVVRLMPQQYWWSYPRYRRR
                     PPGAAPFYS"
     gene            1707..2147
                     /gene="dtd"
                     /locus_tag="VHE37_02140"
     CDS             1707..2147
                     /gene="dtd"
                     /locus_tag="VHE37_02140"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HVT34356.1"
                     /translation="MLGLLQRVNRASVSIRGNCVAGIDRGLLVLVGVERHDDEGKAGR
                     LIERLLGYRVFPDEQGRMNRSLAEIKGGLLLVPQFTLAADTRKGTRPGFSTAAEPARA
                     LELFAALVDCARSAHANVGAGEFGADMQVELINDGPVTFLLQVP"
     gene            2289..3854
                     /locus_tag="VHE37_02145"
     CDS             2289..3854
                     /locus_tag="VHE37_02145"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HVT34357.1"
                     /translation="MKSIWPCGLLAFFVGLQAQAAPTPEAAARFLTQSTFGPTRADIA
                     HVVDIGYGAWLNEQFNLAPSLHGPQLEAPLFSGTPSQDDRQEVWWRESVTAPDQLRQR
                     VAFALSEIMVVSEVNGRLNDQPVLLAEYYDILLRDAFGNFRDLLEDVTLSPAMGNYLS
                     MIYNSSATPKGARPNENYAREVLQLFSIGLIQLKANGKPKLDAGGNALPTYDQNTIEN
                     FARAFTGWSWADSKAFYDPGSSPAPMKPFENHHDYGAKTLLNGVQVPAGLSAAQDLKR
                     ALDNIFNHPNVAPFISRRLIQRLVASDPSPAYIQRVAQVFKDNGHGVRGDLQAVIRAV
                     LTDDEARRPPANNVGKLREPLLRLTAIWRAFDAAAANGRYEYPHPEFDWTESPLGSPS
                     VFNFFQPDYPLPDSDSGLYAPEFQLQTDTQSVTMVNALSRFTRDQASCNAPPADSAQI
                     ELNLCSLAKRSGSPASLVDAVGLLLTYGQLSSDSRTAIIDYLKTVPLGDGTERAREAI
                     FLIASSSEFAVQK"
     gene            3863..5284
                     /locus_tag="VHE37_02150"
     CDS             3863..5284
                     /locus_tag="VHE37_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVT34358.1"
                     /translation="MSNGYTRRDLLKAAGYAGLGLPTAGMLGCSEGGTGAGTPASQLR
                     SVSVAARSYASGISDYKALVCIYLIGGNDAYNMLVPSTPELYGLYAKARPQLALPQDK
                     LLPLNGTAADGGTYSAHPGMSELAALYNAGQAALLSNVGNLLSPTSRADYDAGRVPPS
                     LFSHLDQANQWQSTPGESSILTGWAGRAADVLAGVNGSSELPLNLSIAGADTLQRGIG
                     DSAFVMSPDGVTALDNLPVGTASLRATFNRLLAMSRSNPLAQVHDATLARGIRLNSLI
                     GSLLRKQPRLATRFPGTTLGAQLRLVAQLIGLREALGMKRQIFYVAMGNNPDSFGAFD
                     THFHQLLAQPRLFKQLSEGIKAFYDATVELGVAGNVTSFTASDFGRSLVENENNGTDH
                     GWGGHHWIVGGAVRGSRIYGTPPSLEVDGADDTDNGRFIPSTSVEEYGATLIRWLDVA
                     DSDLDSVFPNLYRFGSRDMGFMT"
     gene            5367..5960
                     /gene="hisB"
                     /locus_tag="VHE37_02155"
     CDS             5367..5960
                     /gene="hisB"
                     /locus_tag="VHE37_02155"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HVT34359.1"
                     /translation="MSSRTASVKRDTRETQISVELNLDGSGRAEFATGIAFLDHMLDQ
                     VARHGLIDLKITAKGDLDIDDHHTVEDVGITLGQAFDQAIGDKKGIIRYGHAYVPLDE
                     ALSRVVVDLSGRPGLEYFVEYPRARVAGFDVDLFREFFQGFVNHAKVTLHIDNLRGRN
                     AHHVAETIFKAFGRALRMACARDARLGDVTPSTKGTL"
     gene            5988..6629
                     /gene="hisH"
                     /locus_tag="VHE37_02160"
     CDS             5988..6629
                     /gene="hisH"
                     /locus_tag="VHE37_02160"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HVT34360.1"
                     /translation="MSSAIGVIDYGMGNLHSLGKALERVAGSTRVVISYEPEELLKCD
                     RLVLPGVGGVLACMNELKRLQLNELVLEAARKLPMLGICLGMQVMLEWSDENGGVPAL
                     GLFPGKVTRFPDPPDDPAYPRLKVPHMGWNRVKQTRPHALWQGVADDSWFYFVHSYHA
                     APSEPAHVLGTADYIKTFPAAIARDNIAAFQFHPEKSQQAGMALLANFVTWNP"
     gene            6770..7492
                     /gene="hisA"
                     /locus_tag="VHE37_02165"
     CDS             6770..7492
                     /gene="hisA"
                     /locus_tag="VHE37_02165"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HVT34361.1"
                     /translation="MLLIPAIDLKGGHCVRLRQGKMDDVTVFSKEPVAVAQRWADEGA
                     QRIHLVDLDGAVKGHPVNVKVIEEIAAKIKVPVQIGGGIRDEATVQRYLNAGVSYVII
                     GTKAVNSPHFLRDLCIEYPRHIIVSLDAKDGRLALNGWAKITHHDAIEMAQHCERDGV
                     EAIIYTDIAMDGMKKGFNVDSTAKLARAVKTPVFASGGVSRMEDIVKLKELEPDGIAG
                     AVIGRALYEGDLNLKDCLKAVQ"
     gene            7530..>7736
                     /locus_tag="VHE37_02170"
     CDS             7530..>7736
                     /locus_tag="VHE37_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HVT34362.1"
                     /translation="MVLAKRIIPCLDIDRGRVVKGVKFEDLVDAGDPVEVARRYDQQG
                     ADELVFLDITASHEDRPILFQTVET"
BASE COUNT         1483 a         2522 c         2377 g         1354 t
ORIGIN      
        1 cgatatcgat caattcaagc tgatcaacga cacccatggt cacctgtttg gcgacgaggt
       61 gatccgcaag gtcggccagg ccatcagcgc gcactacgca ttgcgcccca acgatctcaa
      121 ggcgcgcttt ggtggcgagg aattcgtgct gctctggtac caggtttccg gcgcgcactt
      181 cggacagctg gtcgagagac tgctgtcggc catccgccag ctcgaattgc cgggcagcga
      241 cacggaactg gtgcgcatca ccgccagcgc cggcgcattg tggctggtgc ccgattcgag
      301 ccggcccgct gaagccatcc tcaaccaggc agaccagctg ctctatcagg ccaaagccgg
      361 gggccgcgat cgcatggaat tggtgatact cggcgacccc gcggccaccg acgttgagcc
      421 gggcgatcaa ccgtatactg cgcgccctgc agcgggcctg tagctcagcc ggttagagca
      481 ggggactcat aatcccttgg tcctgggttc gaatcccagc gggcccatgc tcaggaatag
      541 aacggtgctg cgcctggcgg gcgtcgccgg tagcgtggat agctccacca atattgctgc
      601 ggcatcaacc gtacgacctg ctctacgccg cgattgagcg cctcgacgct ttcaccgata
      661 tccgccggtg cggccacaat gtggaagcgg aacccgcgtc cccaggttcg ccgctcggca
      721 agacagaaaa ataccggcgc gccggtgcgc gcggccagct tggtcacgag cgacggcgag
      781 tgcgcgatgg caccgaacag cggcgcaaat tcgccggagc cttcctgcgg atcgtgatcc
      841 ggcaggatgc cgatcaactg ccgatccttg agcgcctgca gcagggcgcg aacgccacca
      901 ccttcggtcg gcaccaatct ggcccccagg cgctggcggc cctgcaatat cagcgcgtcg
      961 attgcgcctt tctgcggctt gtacagcgag gtgatgccgg cgtgggctgc gcagaacatg
     1021 ccggccagct cccacgaacc caggtgcgga cacaatgcga ttgcagcccc gttgcgcatg
     1081 tagcccagca acgcgcgttg ggcctgatcg tcgcgcagcc agcggcgcaa gcggaacgcc
     1141 ggaccgaacc agatggcggg agcctcggcg atcgcctttc ccgattcgag aaaactggtt
     1201 cgggcgatat gacggcgcgc ggcagcgtca aggtcaggga atgcgagctg aacgtgacgc
     1261 agcgtaatgc gcttctgccg ggtcggcagc agccacagca gtccgcccag ggccgcgccg
     1321 gcgccgtgca gcacgggcag cggcagccag ccaatcgcaa ccaggcagta acgcagcagg
     1381 gtcgtcatga aacgcggatt atttcggaac gtcgcgtttc gtgcagaatt tgcttgatag
     1441 gttacgcaac accgccgcat cgcgccgagt aataaagacc cagctcgctt gctggacatg
     1501 acagcggtgc ctgcctgccg tgcccgacgc gggatttcgg acaaaaaaaa ggccagctgg
     1561 ggggctggcc ataggaggag gaaaaaccat gtgccgcgtc tgcgtagaaa ggaggtaact
     1621 acacgagcgc cagcgggaga aagtatccat gccgaccggt ttgtaaaccg tcctacgaac
     1681 gcgacggccc atgtaggatt tttgccatgc tcggactact gcagagagtg aaccgtgcca
     1741 gcgtttccat acgcggaaat tgcgtggccg gaatcgaccg tggcctcctc gttttggtcg
     1801 gagtggagcg ccatgacgac gaaggcaaag caggccgcct gatcgagcgc ctgctcgggt
     1861 atcgggtgtt cccggatgaa cagggtcgca tgaaccgctc actcgctgaa atcaagggcg
     1921 gcttgctgct ggttcctcaa ttcacgctgg ctgcggacac ccgcaaggga acgcggccgg
     1981 gattctccac tgcagcagag ccggcgcggg cgctcgaatt atttgccgcg ctggtggatt
     2041 gcgccagatc cgcgcatgcg aatgttggag ccggcgaatt cggcgccgac atgcaggttg
     2101 agctgatcaa cgacggcccc gtcacgtttc ttctgcaggt gccttagacg ctgcaactcg
     2161 taccgcttgg cggttttgcg ggccgcgtca ccaacagcgc gccagaggca tgctaatttc
     2221 cgcgggcaat tttttccggg cactgggaac ccggactatg tcagcccaaa aattgcaagg
     2281 gagcgcggat gaaatcaatt tggccttgcg gcctcctggc attttttgtc ggcctgcagg
     2341 cgcaggctgc gccgacgccg gaagccgctg cgcgattcct tacgcaatcc acctttggcc
     2401 ctaccagagc cgatatcgcg cacgtggtcg atatcggtta cggcgcctgg ctcaacgaac
     2461 agttcaacct tgcgccgtcc ctgcatggcc cgcagctgga agcccccctt ttcagcggaa
     2521 cgccgtcgca ggacgatcgc caggaagtgt ggtggcgcga atcggtcacg gcacctgacc
     2581 agttgcggca acgcgtcgca tttgcgctgt ccgagatcat ggtggtgtcg gaggtcaatg
     2641 gacggctcaa cgaccaaccc gtgctgctcg ccgagtatta cgacatcctg ctgcgcgatg
     2701 ccttcggcaa ttttcgcgac ctgcttgagg acgtcacgct cagcccggcc atgggcaact
     2761 acctgtccat gatctacaac agcagcgcga ctccgaaggg cgctcgcccc aacgagaatt
     2821 atgcgcgcga agtgctccag ctgttttcca tagggttgat ccagctgaag gccaatggca
     2881 agcccaaact cgatgccggc ggcaacgcct tgcccaccta tgaccagaac accatcgaga
     2941 acttcgcccg cgcattcacc ggctggagtt gggcggacag caaggctttc tacgacccgg
     3001 gcagttcccc agcgccgatg aagcccttcg aaaatcacca cgactatggc gccaagaccc
     3061 tgctcaatgg cgtgcaggta cccgccggac tgagcgccgc gcaggacctc aagcgggcgc
     3121 tcgacaacat cttcaatcac cccaacgttg cgccgttcat ttcgcgccgc ctgatccagc
     3181 ggctggtggc gagcgatccg tcgccggcct acatccagcg cgtggcgcag gtgttcaagg
     3241 acaacggcca cggcgtgcgc ggcgatctcc aagctgtgat ccgcgctgtg ctgaccgatg
     3301 atgaggcgcg ccgcccgcct gcaaacaatg tcggcaaact gcgcgaacct ctgctgcggc
     3361 tgacggccat ctggcgcgcc ttcgatgcag cggctgccaa cgggcgctac gaatatcccc
     3421 atccagagtt tgactggacc gagtcgccgc tgggttcgcc ctcggtgttc aacttcttcc
     3481 agccggatta cccgctgcct gacagcgaca gcggcctgta cgcgccggaa ttccagctgc
     3541 agaccgatac gcagagcgtg accatggtca atgccttgtc gcgtttcacc cgtgatcagg
     3601 cctcgtgcaa cgcgccgccg gccgattccg cgcagatcga actcaacctg tgcagcctcg
     3661 ccaaacgctc gggcagtccg gccagcctgg tggacgcggt cggactgctg ctcacttatg
     3721 gccaactctc aagtgactcg cgtaccgcga tcatcgacta cctcaagaca gtcccgctgg
     3781 gcgatggcac ggagcgggct cgggaagcga ttttcctgat cgcgtcttcc tccgaatttg
     3841 cggtgcagaa ataggtgccg acatgagcaa cggctacacc cggcgtgact tgctcaaggc
     3901 ggccggctat gccggcctgg ggctgccgac ggccggcatg ctcggttgca gcgagggcgg
     3961 gaccggcgca ggcacacccg cctcgcagct gcgctcggtc agcgttgccg cccgcagcta
     4021 tgccagcggc atttcggatt acaaggcgct ggtctgcatc tacctgatcg gcggcaatga
     4081 cgcctacaac atgctggtgc cgagcacgcc ggaactctat gggctgtatg cgaaggcgag
     4141 accccagctg gcgctgcctc aggacaagct gcttccgctc aatggcacgg cggccgatgg
     4201 cggcacttac tcggcacatc ctggcatgag cgagttggcg gccttgtaca acgcaggcca
     4261 ggcagcgctg ctgtcgaatg taggcaatct tctgtctcca acatcgaggg ccgactacga
     4321 cgccggccgc gtaccgccga gcctcttttc acacctggac caagcgaatc aatggcagtc
     4381 gacgcctggc gaatccagca tccttaccgg ctgggcgggc cgcgcggcgg atgtactggc
     4441 cggcgtcaat ggcagcagcg aacttccact caacctgtcg attgcgggcg ccgacacctt
     4501 gcagcgcggc attggcgatt cggcattcgt catgtccccg gatggcgtca ccgccctcga
     4561 caatctgccg gtgggcaccg ccagcctgcg cgctaccttc aaccgcctgc tcgccatgtc
     4621 gcgtagcaat ccgctggcac aggtgcatga cgccaccttg gcgcgcggca tccgcctgaa
     4681 cagcctgatc ggaagcctgc tgcgcaagca gcccaggctc gccacgcgat ttccgggaac
     4741 cacgcttggc gcacaactga ggctggtggc gcagctgatc ggcctgcgcg aagcactggg
     4801 catgaagcgt cagatttttt acgtcgccat gggcaacaac ccggattcct tcggcgcgtt
     4861 cgacacgcat ttccaccagt tgctggccca accacggctg ttcaagcagt tgtccgaagg
     4921 catcaaggca ttctacgacg ctaccgtgga gctgggcgtg gccggcaacg tcaccagttt
     4981 caccgccagc gacttcggcc gcagcctagt cgagaacgag aacaacggca ccgaccatgg
     5041 ctggggcggg caccattgga tcgtcggcgg cgccgtgcgc ggcagccgca tctatggcac
     5101 gccgccgtcg ctcgaggtcg atggcgccga cgatacggac aacggccgct tcatcccctc
     5161 cacatcggtg gaggaatacg gtgcgacgct gatccgctgg ctcgacgtgg ccgactccga
     5221 cctcgactcg gtattcccca atctttaccg cttcggcagc cgcgacatgg ggttcatgac
     5281 ctgataattg gctgccggta ctcggtacaa ttttgctgca ctgcagagta tcctagccgc
     5341 cctttgccgg aagccccgtg cacccgatgt cgtcccgcac cgccagcgtc aagcgcgata
     5401 cgcgcgaaac ccagatcagc gtcgaactga atctggacgg cagcgggcga gcggaattcg
     5461 ccaccggcat cgccttcctc gaccatatgc tggaccaggt ggcacggcac ggactgatcg
     5521 acctcaagat cactgcgaag ggcgatctgg acattgacga tcaccacacg gtggaagacg
     5581 tcggcatcac gctgggccag gctttcgacc aggccatcgg cgacaagaag ggcatcattc
     5641 gctacggcca tgcgtacgtg cccctggacg aggcgctgtc gcgcgtagtg gttgacctgt
     5701 cggggcggcc ggggctggaa tacttcgtcg aatacccgcg ggcgcgcgtt gccggcttcg
     5761 acgtcgacct gttccgcgag ttcttccagg gcttcgtcaa ccatgccaag gtcacgctgc
     5821 atatcgacaa cctgcgtgga cgcaatgcac accatgtggc cgaaaccatc ttcaaggcgt
     5881 tcgggcgtgc attgcgcatg gcctgcgcgc gcgacgcccg cctcggcgac gtaacccctt
     5941 cgaccaaggg cacactctaa aaacatcgcg gcatcctcac gctacgcatg tcttccgcca
     6001 tcggcgtcat cgactacggc atgggcaacc tgcactcgct cggcaaggcg ctggagcggg
     6061 tggcgggcag cacccgtgtg gtcatcagct acgagcccga agaactgctc aagtgcgatc
     6121 gactggtgct gcccggcgtg ggcggcgtgc ttgcctgcat gaacgagctc aagcgcctgc
     6181 agctcaatga actggtcctc gaagccgcgc gcaagctgcc gatgctcggc atctgcctgg
     6241 gcatgcaggt gatgctggaa tggagcgacg agaatggcgg ggtgcccgca ctcggactgt
     6301 ttcccggcaa ggtgacacgc tttcccgatc cgccggatga tccggcttat ccgcgtctca
     6361 aggtccccca catgggctgg aaccgggtca aacagacgcg cccgcatgca ctgtggcagg
     6421 gcgtggcgga cgacagctgg ttctacttcg tgcacagcta ccatgcggcg ccgagcgagc
     6481 cggcccatgt gctcggcacg gcggactaca tcaagacatt ccccgctgcc atcgcccgcg
     6541 acaacatcgc cgcgttccag tttcaccccg agaaaagcca gcaggccggc atggcgctgc
     6601 tcgccaattt tgtcacctgg aatccgtgag tgccgtcata tccggctgac ttgttttcgt
     6661 tccgcgattg gtgcagagtt ttcgcaacgt caggaatacg aacaagcgcc tacgcatcag
     6721 cagtagcacc ggattcaatc catctccctg acctccaccg ctgctctcca tgttgctgat
     6781 ccccgcgatt gatctgaaag gcggccactg cgtgcgcttg cgccagggca agatggacga
     6841 cgtcaccgtc ttctccaagg aaccggtcgc cgtggcccag cgctgggcgg acgaaggcgc
     6901 acagcgcatc cacctggttg atctcgacgg cgcggtcaaa gggcatccgg tcaacgtcaa
     6961 ggtgatcgag gagatcgccg ccaagatcaa ggtgccggta cagatcggcg gcggcatccg
     7021 cgacgaagcc accgtgcagc gctatctcaa cgccggcgtg tcgtacgtga tcatcggcac
     7081 caaggccgtg aactcgccgc atttcctgcg cgacctgtgc atcgagtacc cacgccatat
     7141 catcgtctcg ctcgatgcca aggacgggcg tctcgcgctc aacggctggg ccaagatcac
     7201 ccaccacgac gccatcgaga tggcgcagca ctgcgaacgc gacggcgtcg aggcgatcat
     7261 ctacaccgac atcgccatgg acggcatgaa gaagggcttc aacgtcgatt ccaccgccaa
     7321 gctggcgcgc gcggtgaaga caccggtatt cgcctccggc ggcgtgtcgc gcatggaaga
     7381 catcgtcaag ctcaaggaac tggagccgga cggcatcgcc ggcgcggtca ttggccgcgc
     7441 cctgtacgaa ggcgatctga atctcaagga ctgcctcaag gccgtccagt aacactgccg
     7501 gcgccagggg tgccggaccc ggggaaatca tcgtgctggc caaacgcatc attccttgcc
     7561 tggacatcga ccgcggccgc gtggtcaagg gcgtcaagtt cgaagacctg gtcgacgccg
     7621 gcgacccggt ggaagtcgcg cgccgctatg accagcaagg cgcggatgaa ctggtgttcc
     7681 tcgacatcac cgccagccac gaggatcgcc cgatcctgtt ccagacggtg gaaaca
//
LOCUS       DASZZC010000090         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_327314,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000090 DASZZC010000000
VERSION     DASZZC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327314"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..876
                     /locus_tag="VHE37_02175"
     CDS             226..876
                     /locus_tag="VHE37_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34363.1"
                     /translation="MAAKRQAKETKKRAYLAKDDRRKALLETAARVVEEQGWSALSMI
                     SVAEQAKVSRQLVYAHFASADELMTETMAQIFRDAYDNARDSIQRSSANIVALIEQIE
                     KSTFDMPPGRVRALWQMITATYSDSAETARLSRRLRHLLTNMWAPVANEAFGLEAREG
                     RALIWMLHMAFWGAHQLVDEGEVDRATATRLVSWMVTQIQAGSVMSPLPRAGKNGN"
     gene            complement(992..2635)
                     /locus_tag="VHE37_02180"
     CDS             complement(992..2635)
                     /locus_tag="VHE37_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVT34364.1"
                     /translation="MKKTLGIIALVLVALLATAAANKRYLVSHALGWLTDFKHPRAPN
                     HPVPWQAGPDAAPAGTRPPNVVVILADDLGFNDVSLYGHGVPQSPTPAIEALAKSGVR
                     FDRGYAGNAVCAPSRATLLTGRYSSRFGFEYTPTPGNMAKAAPMFADPTKLHHIVTHP
                     EVTDRIPGFDELGLPPSEVTVAKLLQGRGYHTVHIGKWHLGGTPNLRPLAEGFDESLY
                     MESGLYLPEDSPDVVNSKQEFDPIDQFLWPNMRYATSYNGGPWFEPKGYLTDYYTDEA
                     IHAIHANRNRPFFLYLAHWAVHTPMQASKEDYDALSNIPSHRERVNAAMVRALDRSVA
                     RVMQALKDEGLDQNTLVIFTSDNGAPNYIGIPTVNQPYRGWKLTYFEGGLHVPFAAAW
                     PGHIPAGSVYDQPVMSVDILPTALAAAGITPPTDRVYDGVDLLPYLNGQKSGPPHDVL
                     FWRDGSYQVVLARGWKLQTSERPKKEWLFDLTSDPLEHKNLADEQPAKRAELEKLLAD
                     HNAQMAQPMWPSFIEMPVSVDKTLDQVETPEDEYVYWQN"
     gene            complement(2632..3291)
                     /locus_tag="VHE37_02185"
     CDS             complement(2632..3291)
                     /locus_tag="VHE37_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34365.1"
                     /translation="MSKGSRILVAAWGALYLAALAWYGGHGAPMTAQEKDALFALIQQ
                     RAEHEPLKDGHLLEELHRLADSDDGDEFYMLNLIRYREHAAYPPGYDYDGDVLAADAR
                     YNRAIAPYLFRHGGVPVFLGTPEGRFIDGEGDETWQRVAIVRYRSRRDLLAMVADLAG
                     QDVAVHKWASIEKTQVFPVRAMFSLVMVRLLLATVFVALGLLLHLLLRNAAWYRGRSP
                     A"
BASE COUNT          607 a         1130 c         1205 g          625 t
ORIGIN      
        1 cgagatggaa cgcaacgtgc aggacatgat cgccgccggc tcctggggcg tgcccacgct
       61 gcgcctgccc ccgtcggcgc tcggcggcga tttcaccgtg tggggccagg accgcatctg
      121 gctgatcgag gaagagatcg cccgccgcct gggtgccacc tgagccctcg caaaaaatat
      181 ttacatcatg tataaaagat tcaggatcca ccggctctga gctccatggc ggccaagcgg
      241 caagcgaagg aaaccaagaa gcgcgcctac ctggcgaagg acgaccgccg caaggcgctg
      301 ctggaaaccg cggcgcgggt ggtggaggag cagggctggt cggcgctgtc gatgatctcg
      361 gtggccgagc aggccaaggt cagccgccag ctggtctacg cgcatttcgc ctcggccgac
      421 gagctgatga ccgagaccat ggcgcagatt ttccgcgacg cctacgacaa tgcgcgcgac
      481 agcatccagc gcagttcggc caacatcgtg gcgctgatcg agcagatcga gaagtccacc
      541 tttgacatgc cccccgggcg cgtgcgcgcg ctgtggcaga tgatcaccgc cacctattcc
      601 gacagcgccg agaccgcgcg cctgtcgcgc cggctgcgcc acctgctgac caacatgtgg
      661 gccccggttg ccaacgaggc tttcggcctc gaggcgcgcg aaggccgcgc actgatctgg
      721 atgctgcaca tggctttctg gggcgcgcac cagctggtcg acgaaggcga agtggaccgc
      781 gccaccgcca cgcggctggt cagctggatg gtcacgcaga tccaggcggg cagcgtgatg
      841 tcgccgctgc cgagagcagg caagaacggg aactgatccc gttgctgtga ccttcattga
      901 aaaagagggt cggcgcgcag gcgccgggga aattcacttt cgcgaaaaaa ctcccgctcg
      961 ccgccctctt ttccaaagag gactcaagac atcagttctg ccagtagacg tactcgtctt
     1021 ccggcgtctc cacctggtcc agcgtcttgt ccaccgacac cggcatctcg atgaacgagg
     1081 gccacattgg ctgcgccatc tgcgcgttgt gatcggccag cagtttttcc agttccgcgc
     1141 gcttggccgg ttgttcgtcg gcaagattct tgtgttccag cggatcgctg gtgagatcga
     1201 acagccattc cttcttcggg cgctccgagg tctgcagctt ccagccgcgc gccagcacca
     1261 cctggtagct gccgtcgcgc cagaacagca cgtcgtgcgg cggtccgctc ttctggccgt
     1321 tgagatacgg cagcaggtcg acgccgtcat agacgcggtc ggtgggcgga gtgatgccgg
     1381 cggcggcgag tgcggtgggc aggatgtcga cgctcatcac cggctggtcg tacacgctgc
     1441 cggcgggaat gtgccccggc cacgcggcgg cgaacggcac gtgcaggccg ccttcgaagt
     1501 aagtcagctt ccagccgcgg tatggctggt tcaccgtggg aatgccgatg taattcggcg
     1561 cgccgttgtc gctggtgaag atgaccagcg tgttctggtc cagcccttcg tccttcagcg
     1621 cctgcatcac gcgcgccacg ctgcggtcga gcgcgcgcac catggcggcg ttcacccgct
     1681 cgcggtgcga gggaatattt gacagcgcgt cataatcttc cttgctcgcc tgcatcggcg
     1741 tgtgcactgc ccagtgcgcc aggtacagga agaacggccg gttgcggttg gcgtggatgg
     1801 cgtggatcgc ctcgtcggtg tagtagtcgg tgagatagcc cttgggctcg aaccacggcc
     1861 cgccgttgta gctggtggcg taccgcatgt tcggccacag gaactggtcg atgggatcga
     1921 actcctgctt gctgttgacc acgtcgggcg aatcttccgg caggtacagg ccgctctcca
     1981 tgtacaggct ttcatcgaag ccctcggcca atgggcgcag gttcggcgtg ccgcccaggt
     2041 gccacttgcc gatgtgcacg gtgtggtagc cgcggccctg cagcagcttg gcgacggtga
     2101 cttccgacgg cggcaggccc agttcgtcga agcccgggat gcggtcggtg acctcgggat
     2161 gggtgacgat gtgatgcagc ttggtcgggt cggcgaacat cggcgcggcc ttggccatgt
     2221 tgcccggcgt cggcgtgtac tcgaagccga agcgcgacga gtagcggccg gtgagcagcg
     2281 tcgcgcgcga aggcgcgcac accgcgttgc cggcgtagcc gcggtcgaag cgcacgccgg
     2341 atttcgccag cgcctcgatg gccggcgtcg gcgactgcgg cacgccgtgg ccgtacaggc
     2401 tcacatcgtt gaagccgagg tcgtcggcca ggatcaccac cacgttgggc gggcgcgtgc
     2461 cggcgggcgc ggcgtcgggc ccggcctgcc acggcaccgg gtggttcggc gcgcgcgggt
     2521 gcttgaaatc ggtgagccag ccgagcgcgt gcgacaccag gtagcgcttg ttcgctgccg
     2581 cggtggcgag cagtgccact aaaaccagcg cgatgatgcc gagggtcttt ttcatgcagg
     2641 actcctgccg cgataccagg cggcgttgcg caacagcaga tgcagcagca gccccagcgc
     2701 tacgaaaacc gtagcaagca gcagccgcac catcaccagg ctgaacatgg cgcgcaccgg
     2761 gaacacctgg gttttctcga tcgaggccca cttgtgcacg gccacgtcct gcccggcgag
     2821 atcggccacc atggccagca ggtcgcggcg gctgcggtag cgcacgatgg cgacgcgctg
     2881 ccaggtctca tcgccctcgc cgtcgatgaa gcggccctcg ggcgtgccga ggaacaccgg
     2941 cacgccgccg tgcctgaaca ggtagggcgc gatggcgcgg ttgtagcgtg cgtcggccgc
     3001 cagcacgtcg ccgtcatagt cgtagccggg cggataggcg gcatgctcgc ggtagcggat
     3061 caggttgagc atgtaaaact cgtcgccgtc gtcgctgtcg gccagccggt gcagttcttc
     3121 gagcagatgg ccgtccttca gcggttcgtg ctcggcgcgc tgctggatca gcgcgaacag
     3181 cgcgtccttt tcctgcgcgg tcatcggtgc gccgtggccg ccgtaccagg cgagcgcggc
     3241 caggtacagc gcaccccacg cggcgacgag aatcctgctg cctttcgaca tggccagttc
     3301 tgctaccgga gcgcgaaaaa tgtgttcacc gcatgcccgt tttgcttgtt aataattgtt
     3361 atatgtttta agaaaaatac aacatttaat aacatcatca agggcagtgc ggtgaggcaa
     3421 aaaatgcgtc atgccagcag cgccgcgcag ccggtggacg ccagcgcggc gcgcacccag
     3481 gcctggtcgt ccacggacag cgcagcgaat tgttcgcgca gccacttcag gcatttcgcc
     3541 tggtagggga aggtctgctg gcgccag
//
LOCUS       DASZZC010000091         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_969326,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000091 DASZZC010000000
VERSION     DASZZC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969326"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VHE37_02190"
     CDS             <1..711
                     /locus_tag="VHE37_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HVT34366.1"
                     /translation="SAQAYGKANALMQPGSADLLANQAEALALANERNLQGQPVQLLE
                     AALKLDPSHVKSLWYAGIAALQRDDKDGAKKYWTMLAVQPELPDDLRTALDEQLQQLG
                     VSAPRPAPAPAAPAPPVSGGLALSVQVELAPNLAAQSREAQTLFVFAKAESGPPMPLA
                     VQRITAPKLPLTLTLDDSMAMMPSLKLSQFDHWIVTARLTRGGTVQPESGDLQGQLTV
                     QKADAGKPVRIVIDQVLP"
     gene            860..>1544
                     /locus_tag="VHE37_02195"
     CDS             860..>1544
                     /locus_tag="VHE37_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HVT34367.1"
                     /translation="MTEVLSAMPSALALYAKAAATARRKPKGKPQIPALDARMDNVRA
                     DAAKLAAYNKVCGFEPGEQLPITYPQVMIAALHMHLMSNAAFPLPMLGLVHVRNHIEQ
                     TRALRADESFTVNVRTGESRDVRQGLEFDIHTEFSAGEETPWRSVMTILYRMKGPKGQ
                     AKAPPVPAPALAEYRNFDAPADIGRRYAKVSGDHNPIHTSAVGAKLFGFPRAIAHGMW
                     TLARVTALVG"
BASE COUNT          284 a          529 c          501 g          230 t
ORIGIN      
        1 tcggcgcagg cctacggcaa ggccaacgcg ctgatgcagc cgggcagcgc ggacctgctg
       61 gccaaccagg ccgaggcgct ggcgctggcc aacgagcgca acctgcaggg ccagccggtg
      121 cagctgctcg aagcggcgct gaagctcgat ccatcccatg tgaaatcgct gtggtacgcc
      181 ggcatcgcgg cgctgcaacg cgacgacaag gacggggcga agaaatactg gaccatgctg
      241 gcggtgcagc cggagctgcc tgacgacctg cgcacggcgc tcgacgagca gctgcagcag
      301 ctgggcgtgt ctgcgcccag gcccgcaccg gcgcctgcgg cgcccgcgcc gcccgtgtcg
      361 ggtggccttg cgctgagcgt gcaggtggaa ctcgcgccga atctggcggc tcagtccagg
      421 gaagcgcaga cgctgttcgt gttcgccaag gccgaaagcg gcccgccgat gccgctggca
      481 gtgcagcgca tcaccgcgcc gaagctgccg ctgacgctga cgctggacga cagcatggcg
      541 atgatgccct cgctcaagct gtcgcagttc gatcactgga tcgtcaccgc gcggctcacg
      601 cgcggcggca cggtgcagcc ggaaagcggc gacctgcagg gccagctgac cgtgcagaaa
      661 gccgatgccg gcaagccggt gcgcatcgtc atcgaccagg ttttgccctg accgcacagc
      721 cgtttttgat gttataaaat atttatcatt tttgtaaata tacaataata attaacatca
      781 aaacagccgg tgcggtgcat ggtttttcac gcctgatggc tagaatggcg cccctcatac
      841 gctcatccac atcacgacca tgaccgaagt cctgagtgca atgccgtcgg ccctcgcgct
      901 atatgccaag gccgcggcca ccgcgcggcg caagcccaag ggcaagccgc agatcccggc
      961 gctcgatgcg cgcatggaca acgtgcgcgc cgacgcggcg aagctggcgg cctacaacaa
     1021 ggtctgcggc ttcgagcccg gcgagcagct gccgatcacc tacccgcagg tgatgatcgc
     1081 ggcgctgcac atgcacctga tgtccaacgc ggcgtttccg ctgcccatgc tcggcctggt
     1141 gcacgtgcgc aaccacatcg agcagacgcg cgcgctgcgc gccgacgaga gcttcaccgt
     1201 gaacgtgcgc accggcgaat cacgcgacgt gcgccagggc ctggagttcg acatccacac
     1261 cgagttttcc gccggcgaag aaacgccatg gcgctcggtg atgaccattc tctaccgcat
     1321 gaagggcccc aaggggcagg ccaaggcgcc gccggtgccg gcgcccgcgc tggcggagta
     1381 ccgcaacttc gacgcaccgg ccgacatcgg ccgccgctat gccaaggtct ccggcgacca
     1441 caacccgatc cacaccagcg cggtcggcgc caagctgttc ggttttccgc gcgccatcgc
     1501 gcacggcatg tggacgctgg cgcgcgtcac cgcgctggtc ggcg
//
LOCUS       DASZZC010000092         6943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_843157,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000092 DASZZC010000000
VERSION     DASZZC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843157"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..659
                     /gene="lolB"
                     /locus_tag="VHE37_02200"
     CDS             72..659
                     /gene="lolB"
                     /locus_tag="VHE37_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HVT34368.1"
                     /translation="MRWRIALGAALLLAGCAGEIKPDLNQQQAQQAWQQRSQALAQLR
                     QFNLAGRIASTSASGNLSWQQHADGSFELHLSGPLGAGAMSISGTERWVEVRTSKGTH
                     ASGDPQAWIYQQTGWQLPVTGLRWWVLGLPSPHSPAHVELDGGGRAASIDQDGWHLAY
                     SEYQELDGRTLPRRFEAVNGEAKIKLVADRWTGLE"
     gene            656..1507
                     /gene="ispE"
                     /locus_tag="VHE37_02205"
     CDS             656..1507
                     /gene="ispE"
                     /locus_tag="VHE37_02205"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HVT34369.1"
                     /translation="MTRGLPDTWPAPAKLNLFLHVTGRRADGYHLLQTVFQFIDVCDT
                     LAFRPRSDAAIRCVEGAPGVADDDNLAVRAARALQAASGATHGADIGMVKRIPLGAGL
                     GGGSSDAATTLVALNAIWNLGLSIDRLAQIGLSLGADVPVFVRGHAAWGEGIGEKLQP
                     VELPEPWYLVLVPPVAVPTREVFTAPALKRDSAPIALSDFLAGAGRNDCEPVTRALYP
                     QVGEALDWLGRRAPARMSGTGAAVFAAFATRAEAEVIAETAPNRWRCFVAQGRNRSPL
                     ADIMRRV"
     gene            1520..1594
                     /locus_tag="VHE37_02210"
     tRNA            1520..1594
                     /locus_tag="VHE37_02210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1552..1554,aa:Gln,seq:ttg)
     gene            1648..2586
                     /locus_tag="VHE37_02215"
     CDS             1648..2586
                     /locus_tag="VHE37_02215"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HVT34370.1"
                     /translation="MMLFSGNANPKLAKDMASYLKMPLGQADVGRFSDGEVQVEILEN
                     VRGRDVFVIQPTCVPTNDNVMELMMMLDALKRASASRLTAVVPYFGYARQDRRPRSTR
                     VPISAKVVADMLGEVGASRVLTVDLHADQIQGFFDIPVDNVYSSPVLLGDIWRQKYDK
                     LIVVSPDVGGVVRARAIAKTLDDADLAIIDKRRPKANESKVMNIIGDVTGKVCVMVDD
                     MVDTAGTLCKAAGALKEHGAVKVVAYVTHPVLSGPAVTNISNSLLDELVVTDTIPLSP
                     AAAACPKIRQLSIAGLLAETIRRISAEESVSSLYVD"
     gene            2586..3365
                     /locus_tag="VHE37_02220"
     CDS             2586..3365
                     /locus_tag="VHE37_02220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT34371.1"
                     /translation="MPAVHTLDKSGIRRFALQQRAAGAYYDRAQAHLEDNFRGSIDRF
                     CDIAWEFRGCPRVLDAGSGDGLLLALLKMLGHQVHAVDQSDRSKTDIYTRHAIPFAVC
                     NIEADPLPFADGTLDAVSCCQAFEHFTHAHIRPLLEMRRVLAEGGLVEIDVPNVAAFR
                     NRWRLLRGKHITYGYADHYLRTEPVVYKGREYYPDRHNREFTRDELELLLREAGFRGV
                     DVRFLRSRRYREGWEQLRSLGTALKDAVPSLRKSLIGFGRK"
     gene            complement(3366..4142)
                     /locus_tag="VHE37_02225"
     CDS             complement(3366..4142)
                     /locus_tag="VHE37_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34372.1"
                     /translation="MWTRALTRTKIRAALAHFSLSLLIFAPILFLVVRRWYPGPLFFT
                     DGGWQGTRIMVLIDLVLGPFLTFTVFNPAKTRLALGVDFGFIGTVQIAALVYGVMNIS
                     STAIEAVSWYEGAFVAVSASDYRKQNIAPQDWAKFGDGPPYWVYARTPLPADLGEVMS
                     RGLGQNIAPAAQFNLTSPLREHLADLAAGEIRPQTVAGSDAPLAQRLNDFAAGHGGAD
                     GVHFYAMNGFYRSVIVALDGQGRYLGFVFHEPPAPPPPKK"
     gene            4408..5115
                     /locus_tag="VHE37_02230"
     CDS             4408..5115
                     /locus_tag="VHE37_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HVT34373.1"
                     /translation="MKESFVVNAQPRVAGGTGASRRLRKTGQVPAILYGGKDQPAAIQ
                     VSANEMWKHLQTEAFYSHILTLKLEGQEQQVVLKDMQRHPVTENPMHIDFQRVLADVA
                     LRMQVPLHFKGSEVAPGIKTGGGIVEHHMNSVEVECLPRFLPEFIEVDMSAMELNQSI
                     HLSQLKLSEGVALVELKHGKDPSVVAIHLPRAVVEEEPAAAEAPSAEVPATAQKAPEA
                     AEAGKGDAKKDEKKDKK"
     gene            5176..5757
                     /gene="pth"
                     /locus_tag="VHE37_02235"
     CDS             5176..5757
                     /gene="pth"
                     /locus_tag="VHE37_02235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HVT34374.1"
                     /translation="MPSELRAIIGLGNPGRDYADTRHNAGFWFADAFARAHGGSFRTE
                     SKFRGELARVKAAGADVLLLKPSTYMNLSGEAAQLLAQFYKLAPGDMLVAHDELDLPV
                     GTVRLKKAGGHGGHNGLRSLTQHLGADYLRLRIGVGHPGRKEMVMPYLTEMRITRSDE
                     TLVMDGIARALPAVETWLAGDWNKAMQQLHSES"
     gene            5761..6855
                     /gene="ychF"
                     /locus_tag="VHE37_02240"
     CDS             5761..6855
                     /gene="ychF"
                     /locus_tag="VHE37_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HVT34375.1"
                     /translation="MGIKCGIVGLPNVGKSTLFNALTKAQIAAENYPFCTIDPNVGVV
                     AVPDPRLDELAAIVKPQRILGAAVEFVDIAGLVEGASEGKGKGNAFLSHIRETDAITH
                     VVRCFVDDDIIHVMEKVDPIRDAEVINLELAMADLDIIAKAAERAGKNAKTGAKEAIA
                     KHELLKRMQAHLDGGQPARSLKLTPEEEPQLREYNLITAKPMLYIANVDEAGLKDGNE
                     YLARVQELAKKEGAEVVAVCAAMEAEIAQLDDADKLDFLKDLGLSEPGLNRVIRAAYN
                     LLGLQTYFTAGVQEVRAWTVRKGATAPQAAGVIHTDFEKGFIRAEVIGYADYIAGKGE
                     AGAKEAGKWRLEGKDYVVAEGDVMHFRFNV"
BASE COUNT         1237 a         2328 c         2316 g         1062 t
ORIGIN      
        1 tgggacaagg cgctggccag cgatcccgac cacccggtgc tgaaggaaac cgtgaagcgg
       61 ttcgtgcgct gatgcgctgg cgcatcgcgc tcggcgccgc gctgctgctg gcgggctgcg
      121 ccggcgaaat caagcccgac ctgaaccagc agcaggcgca gcaggcctgg cagcagcgca
      181 gccaggcgct ggcgcagctc cggcagttca acctcgcggg gcgcattgcc tcgaccagcg
      241 cgtcgggcaa tctcagctgg cagcagcacg ccgacggcag cttcgagctg cacctgtcgg
      301 gcccgctggg cgcgggcgcc atgtcgatca gcggcaccga gcgctgggtg gaagtgcgca
      361 ccagcaaggg cacgcatgcc agcggtgatc cgcaggcgtg gatctaccag cagaccggct
      421 ggcagctgcc ggtgacggga ctgcgctggt gggtgctggg cctgccctcg ccgcattcgc
      481 cggcgcatgt cgagctcgac ggcggcggcc gcgcggccag catcgaccag gacggctggc
      541 acctggcgta cagcgaatac caggagctcg acggccgcac gctgccgcgg cgcttcgagg
      601 cggtgaacgg cgaagcgaag atcaagctgg tggccgaccg ctggaccggg ctcgaatgac
      661 gcgcggcctg ccggacacct ggccggcgcc ggccaagctc aacctgttcc tgcacgtgac
      721 cggccgccgc gccgacggct atcacctgct gcagaccgtg ttccagttca tcgacgtgtg
      781 cgacacgctc gccttccgcc cgcgcagtga tgcggcgatc cgctgcgtgg aaggcgcgcc
      841 gggcgtggcc gacgacgaca acctggcggt ccgcgccgcg cgcgcgctgc aggcggccag
      901 cggcgcgacg cacggcgccg acatcggcat ggtcaaacgc atcccgctgg gcgccgggct
      961 tggcggcggt tcctccgatg cggcgaccac gctggtagcg ctcaacgcca tctggaacct
     1021 gggactgtcc atcgaccggc tggcgcagat cgggctctcg ctgggcgccg acgtgccggt
     1081 gttcgtgcgc ggccacgcgg cctggggcga agggatcggc gaaaagctgc agccggtgga
     1141 actgcccgag ccctggtacc tggtgctggt gccgccggtg gcggtgccga cgcgcgaggt
     1201 gttcaccgcg ccggcgctga agcgcgacag tgcgccgatc gcgctcagcg attttctcgc
     1261 cggcgccggc cgcaacgact gtgagccggt gacgcgtgcg ctctacccgc aggtgggcga
     1321 agcgctggac tggctgggcc ggcgcgcacc tgcgcgcatg tccggcaccg gcgcggcggt
     1381 gttcgccgcg ttcgccacgc gcgccgaagc tgaagtgatc gccgaaaccg cgcccaaccg
     1441 ctggcgctgt ttcgttgcgc agggccgcaa ccggtcgccc ctcgcggata taatgcgccg
     1501 cgtttgattt ttgccgtttt ggggagtcgc caagtggtaa ggcagcgggt tttgatcccg
     1561 ccattcccag gttcgaatcc tggctcccca gccacttccg ctttaccaga cgatcacggg
     1621 gaacgcgcgc gcatgtccga caccaagatg atgctgttct ccggcaacgc caatccgaaa
     1681 ctggcgaagg acatggccag ctacctgaag atgccgctgg gccaggccga cgtgggccgc
     1741 ttctccgacg gcgaggtgca ggtggagatc ctggagaacg tgcgcggccg cgacgtcttc
     1801 gtcatccagc ccacctgcgt gccgaccaac gacaacgtga tggaactgat gatgatgctc
     1861 gacgcgctca agcgcgcgtc ggcctcgcgg ctcaccgcgg tggtgcccta cttcggttat
     1921 gcgcggcagg atcgccgccc gcgctccacg cgcgttccga tcagcgccaa ggtggtcgcc
     1981 gacatgctcg gcgaagtcgg cgccagccgc gtgctcacgg tcgatctgca tgccgaccag
     2041 atccaggggt tcttcgacat cccggtagac aacgtctatt cctcgccggt gctgctgggc
     2101 gacatctggc ggcagaagta cgacaagctg atcgtggtct cgcccgatgt cggcggcgtg
     2161 gtgcgtgcac gcgccatcgc caagacgctg gacgatgccg atctggccat catcgacaag
     2221 cgccgcccga aggccaacga gtcgaaggtg atgaacatca tcggcgatgt caccggcaag
     2281 gtctgcgtga tggtcgacga catggtcgac accgccggca ccctgtgcaa ggcggcaggc
     2341 gcgctgaagg aacacggcgc ggtgaaggtg gtcgcctacg tgacgcaccc ggtgctgtcg
     2401 ggcccggcgg tgaccaacat ctccaactcc ctgctcgacg aactggtggt gaccgacacc
     2461 atcccgctgt cgccggcggc ggcggcgtgc cccaagatcc gccagctgtc gattgcggga
     2521 ttgctggccg agacgatccg tcgcatcagt gcggaagaat cggtcagctc gctctacgtc
     2581 gactgatgcc ggcggtccac acgctggaca agagcggcat caggcgcttt gccctgcagc
     2641 agcgcgctgc aggtgcgtat tacgaccgcg cgcaggcgca tctcgaggac aatttccgcg
     2701 gctcgatcga ccgcttctgc gacatcgcct gggagttccg cgggtgcccg cgcgtgctcg
     2761 acgccggctc cggcgacggc ctgctgctgg cgctgctcaa gatgctcggc caccaggtgc
     2821 acgcggtgga ccagtcggac cgcagcaaga ccgacatcta tacgcgccac gccattccgt
     2881 tcgcggtctg caacatcgag gccgacccgc tgccgtttgc cgacggcacg ctcgacgcgg
     2941 tgagctgctg ccaggccttc gagcatttca cccacgcgca tatccggccg ctgctggaga
     3001 tgaggcgtgt gctggccgag ggcggcctgg tggagatcga cgtgcccaac gtggccgcgt
     3061 tccgcaaccg ctggcgcctg ctgcgcggca agcacatcac ctacggctac gccgatcact
     3121 acctgcgcac cgagccggtg gtctacaagg gccgcgagta ctacccggac cggcacaacc
     3181 gcgagttcac ccgcgatgaa ctggaactgc tgctgcgcga agccggcttc cgcggcgtgg
     3241 atgtgcgctt tctccggtcg cgccgctacc gcgaaggctg ggagcagttg cggtcgctcg
     3301 gcaccgcgct gaaggacgcg gtgccctcgc tgcgcaaatc gctgatcggc ttcggccgca
     3361 agtaatcact tcttcggcgg cggcggcgcg ggcggctcat ggaagacgaa gcccaggtac
     3421 ctgccctggc catcgagcgc gacgatcacc gagcggtaga agccgttcat ggcgtagaaa
     3481 tgcacgccgt ccgctcctcc gtggccagcg gcaaagtcgt tgagccgctg cgccagcggg
     3541 gcatcactgc cggcgacggt ctgcggcctg atctcgcctg ccgccaggtc cgccaggtgc
     3601 tcgcgcagcg gcgaggtgag attgaactgc gccgccggcg cgatgttctg gcccaggccc
     3661 ctggacatga cttcgcccag gtcggccggc agcggcgtgc gcgcatagac ccagtacggc
     3721 ggcccgtcgc cgaacttcgc ccagtcctgc ggtgcgatgt tctgcttcct gtagtcggat
     3781 gccgaaaccg cgacgaacgc gccctcgtac cacgacaccg cctcgatggc ggtggaggaa
     3841 atgttcatca cgccgtagac cagcgcggcg atctgcaccg tgccgatgaa accgaagtcg
     3901 acgcccagcg ccaggcgcgt cttggccggg ttgaacacgg tgaaagtgag gaacggcccg
     3961 agcaccaggt cgatcagcac catgatgcgc gtgccctgcc agccgccgtc ggtgaagaac
     4021 agcggcccgg gataccagcg gcgcaccacc aggaacagga tcggcgcgaa gatcaacagg
     4081 ctcaacgaaa aatgcgccag tgcggcgcgg atcttggtgc gcgtgagtgc ccgggtccac
     4141 atacgtcctc cgttacgttt tccagaccgg aattttttaa tgcaagccgc cgccagcttt
     4201 caatagcgcg taaaccggtt accgcaccgg cctcggtcat gttattaaat atataactat
     4261 taaataattg atgtatatta tataacaaca aaaacggtat tgcggtgagt cccgccacgc
     4321 tgtatttacc gccgccgttt gccgtagaat gcgcgcccct cgcgtctggt cgcggacgcg
     4381 ggtttttcaa tttcccagga gtttgccgtg aaagaatcat tcgtcgtcaa cgcccagccg
     4441 cgcgttgcgg gaggaacggg tgcgagccgc cgtctccgca agaccggcca ggtgcccgcc
     4501 atcctgtacg gcggcaagga tcagcccgct gccatccagg tcagcgccaa cgagatgtgg
     4561 aaacatctgc agaccgaggc gttctactcg cacatcctca cgctcaagct cgagggccag
     4621 gagcagcagg tggtgctcaa ggacatgcag cgccacccgg tgaccgagaa cccgatgcac
     4681 atcgacttcc agcgcgtgct ggccgatgta gcgctgcgca tgcaggtgcc gctgcacttc
     4741 aagggctccg aagtggcgcc gggcatcaag actggcggcg gcatcgtcga gcaccacatg
     4801 aactcggtgg aagtcgagtg cctgccgcgc ttcctgcccg agttcatcga ggtggacatg
     4861 tcggcgatgg agctgaacca gtccatccac ctgtcgcagc tcaagctgtc ggaaggcgtg
     4921 gccttggtcg aactcaagca tggcaaggat ccgtcggtgg tggccatcca cctgccgcgc
     4981 gccgtggtcg aggaagagcc ggctgccgcc gaggcgccgt cggccgaagt gccggccacc
     5041 gcgcaaaagg cgccggaagc cgccgaagcc ggcaagggcg acgccaagaa ggacgagaag
     5101 aaggacaaga agtaagtcct tcgccccgag caaatcgaag gccgcgcaag cggccttctt
     5161 ttttcagcca ccgccatgcc atccgaactg cgcgccatca tcggcctggg caatcccggc
     5221 cgcgactacg ccgacacgcg ccacaacgcg ggcttctggt tcgccgacgc gtttgcgcgc
     5281 gcgcatggcg gcagcttccg cacggaatcg aagttccgcg gcgagctggc ccgggtgaaa
     5341 gcagctggcg ccgacgtgct gctgctgaag ccgtccacct acatgaatct ctccggcgag
     5401 gcggcgcaac tgctggcgca gttctacaag ctggcaccgg gcgacatgct ggtggcgcac
     5461 gacgagctcg atctcccggt gggcacggtg cggctgaaga aggccggagg ccacggcggg
     5521 cacaacgggc tgcgctcgct gacgcagcac ctgggcgccg actacctgcg cctgcgcatc
     5581 ggcgtcggtc acccgggccg caaggagatg gtgatgccat acctcaccga gatgcgcatt
     5641 acccgctccg acgaaacact ggtgatggac ggcatcgcgc gcgcgctgcc cgccgtcgaa
     5701 acctggctgg ccggcgactg gaacaaggcc atgcagcaac tacatagcga gagttaagtc
     5761 atgggcatca aatgcggcat cgtcggcctt cccaacgtcg gcaagtccac cctgttcaac
     5821 gcgctgacca aggcgcagat cgcggcggaa aactatccgt tctgcaccat cgaccccaac
     5881 gtcggcgtgg tggccgtgcc cgacccgcgc ctcgatgagc tggccgcgat cgtgaaaccg
     5941 cagcgcatcc tcggcgccgc ggtggagttc gtcgacatcg ccggcctggt cgaaggcgcc
     6001 agcgagggca agggcaaggg caacgcgttt ctctcgcata tccgcgaaac cgatgccatc
     6061 acgcacgtgg tgcgctgctt cgtcgacgac gacatcatcc acgtgatgga aaaggtcgac
     6121 ccgatccgcg atgccgaggt gatcaacctg gaactggcca tggccgacct ggacatcatc
     6181 gccaaggccg ccgagcgcgc cggcaagaac gccaagaccg gcgccaagga agccatcgcc
     6241 aagcacgagc tgctcaagcg catgcaggcg catctcgacg gcggacagcc ggcgcgctcg
     6301 ctcaagctca cgcccgaaga agagccgcag ctgcgcgagt acaacctgat caccgccaag
     6361 ccgatgctgt acatcgccaa cgtcgacgag gccggtctca aggacggcaa cgagtacctg
     6421 gcgcgcgtgc aggaactggc gaagaaggaa ggcgccgaag tggtcgccgt gtgcgcggcg
     6481 atggaagcgg agatcgcgca gctggacgac gccgacaagc tcgatttcct caaggacctg
     6541 ggcctgtccg agccgggcct gaaccgcgtg atccgcgccg cctacaacct gcttggcctg
     6601 cagacctatt tcaccgccgg cgtgcaggaa gtgcgcgcgt ggacggtgag gaaaggcgcc
     6661 accgcgccac aggccgccgg cgtgatccac accgacttcg agaagggctt catccgcgcc
     6721 gaggtgatcg gctacgccga ctacatcgca ggcaagggcg aagccggcgc caaggaagcc
     6781 ggcaagtggc ggctggaagg caaggactac gtcgtcgccg agggcgacgt gatgcacttc
     6841 cgcttcaacg tctgacggcg cctcccgggc ggcagcagtc ggcgcctgct gccggaccgg
     6901 catccgctgc gccggcaatc cgcgatcgtt tcatggactg ccc
//
LOCUS       DASZZC010000093         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_458059,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000093 DASZZC010000000
VERSION     DASZZC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458059"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VHE37_02245"
     CDS             <1..598
                     /locus_tag="VHE37_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT34376.1"
                     /translation="GFAQLLQKRYAGKLDAQADEYFNYMVTSSKQMQALIDGLLEISR
                     VGRDNPFEHVDAEQLLAEVEQQLLSVVVERHAQITHDPLPRLRASPLEFKQMLANLIG
                     NGLKFQPGEAPRVHVSAQRRDGEWQFSVRDYGIGISAEHQEKVFQIFQRLHTAEQYEG
                     TGIGLAICQKIARRHGGRIWVESSLGEGSTFHFTIRDA"
     gene            complement(773..>2557)
                     /locus_tag="VHE37_02250"
     CDS             complement(773..>2557)
                     /locus_tag="VHE37_02250"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HVT34377.1"
                     /translation="AGYIGMNVSVRANVRTAEAARRGMRPAMDVAFRGGAITGMLVVG
                     LGLLGVAGYYGAVLQMTGARDTALHALVGLAFGGSLISIFARLGGGIFTKGADVGADL
                     VGKVEAGIPEDDPRNPAVIADNVGDNVGDCAGMAADLFETYAVTLVATMVLAGLTIAQ
                     FGANAVLYPLVLGGASIIASIVGTFFVKANEGGKIMNALYRGVIVSGVLAAIAFYPIT
                     SAFFPVAATAMNLYWCALIGLALTGIIVWITEYYTGTTFAPVKHIAAASQTGHGTNII
                     AGLGVSMKSTALPVLAVCAAIWGAFHFGTLASTGWGSRELGGLFGIAVAATAMLSMTG
                     MIVALDAYGPITDNAGGIAEMSGLPKEIRNITDPLDAVGNTTKAVTKGYAIGSAGLAA
                     LVLFADYTHNLQAAGHLTTFDLSNHMVIIGLFIGGLVPYLFGAMAMEAVGRAAGSVVV
                     EVRRQFAEIKGIMEGTAKPQYGVAVDMLTKAAIKEMIVPSLLPIAVPLIVGLTLGAQA
                     LGGVLIGTIITGLFVAISMTTGGGAWDNAKKYIEDGHFGGKGSDTHKAAVTGDTVGDP
                     YKDTAGPAVNPLIKIINIVALLMVPLLH"
BASE COUNT          454 a          843 c          853 g          407 t
ORIGIN      
        1 cggctttgcc cagctgctgc agaagcgcta tgccggcaag ctcgacgcgc aggccgacga
       61 atacttcaat tacatggtca ccagctccaa gcagatgcag gcgctgatcg atggcctgct
      121 ggaaatctcg cgcgtcggcc gcgacaaccc gttcgagcac gtggatgccg agcagctgct
      181 ggccgaggtg gagcagcagc tgctgtcggt ggtggtggag cggcacgcgc agatcacgca
      241 cgacccgctg cccaggctgc gcgcctcgcc gctcgagttc aagcagatgc tggccaacct
      301 gatcggcaac ggcctcaagt tccagccggg cgaagcgcca cgcgtgcacg tgagcgcgca
      361 gcgccgcgac ggcgaatggc agttctcggt gcgcgactac ggcatcggca tcagtgccga
      421 gcaccaggag aaggtgttcc agatcttcca gcgcctgcac actgccgagc agtacgaggg
      481 caccggcatt gggctcgcca tctgccagaa gatcgcgcgc cgccacggcg gacgcatctg
      541 ggtggaatcg tcgctgggcg aaggctccac cttccacttc accatccgcg atgcctgaac
      601 actgtttttt tacctgaccg caccccgccc gcttgtgtta taaaatattg tttatatata
      661 aaaatagata atattttata acagtcaaaa tggcagtgcg gtggaggcaa aaaacgcctc
      721 gcgcaaaaca aaaggccccg cgtgcggggc cttttgctga actcgaccga gatcagtgca
      781 gcagcggcac catcagcagt gcaacgatat tgatgatctt gatcagcgga ttcaccgccg
      841 ggccggcggt gtccttgtac ggatcgccca ccgtgtcgcc ggtgaccgcg gccttgtgcg
      901 tgtcggagcc cttgccgccg aagtggccgt cctcgatgta cttcttggca ttgtcccagg
      961 caccgccgcc ggtggtcatg gagatggcca cgaacaggcc ggtgatgatg gtgccgatca
     1021 gcacgccgcc cagcgcctgc gcaccgagcg tcaggccgac gatcagcggc accgcgatcg
     1081 gcagcagcga gggcacgatc atctccttga tcgccgcctt ggtcagcatg tccacggcca
     1141 cgccgtactg cggcttggcg gtgccttcca tgatgccctt gatctccgca aactggcggc
     1201 gcacttccac caccaccgag ccggccgcgc ggccgaccgc ttccatcgcc atggcgccga
     1261 acaggtacgg caccaggccg ccgatgaaca ggccgatgat gaccatgtgg ttcgacaggt
     1321 cgaaggtggt caggtggccc gccgcctgca ggttgtgcgt gtagtcggcg aacagcacca
     1381 gcgcggccag gccggcggag ccgatcgcgt agcccttggt caccgccttg gtggtgttgc
     1441 ccaccgcgtc gagcggatcg gtgatgttgc ggatctcctt gggcaggccg ctcatctcgg
     1501 cgatgccgcc ggcgttgtcg gtgatcgggc cgtaggcgtc gagcgccacg atcatgccgg
     1561 tcatcgacag catggcggtg gcggccacgg caatgccgaa caggccgccc agttcgcgcg
     1621 agccccagcc ggtggaggcc agggtgccga agtggaacgc gccccagatg gcggcgcaca
     1681 ccgccagcac cggcagcgcg gtggacttca tcgacactcc gaggccggcg atgatattcg
     1741 tgccgtggcc ggtctgcgac gcggcggcga tgtgcttcac cggagcgaac gtggtgccgg
     1801 tgtagtactc ggtgatccac acgatgatgc cggtcagcgc caggccgatc agcgcgcacc
     1861 agtacaggtt catcgcggtg gccgccaccg ggaagaacgc gctggtgatc gggtagaagg
     1921 cgatggccgc cagcacgccg gagacgatca cgccgcggta aagcgcgttc atgatcttgc
     1981 cgccctcgtt ggccttgacg aagaaggtgc cgacgatcga ggcaatgatc gaggcgccgc
     2041 caagcaccag cgggtagagc accgcgttgg cgccgaactg ggcgatggtc aggccggcca
     2101 gcaccatggt ggcgaccagc gtcaccgcgt aggtctcgaa caggtcggcc gccatgccgg
     2161 cgcagtcgcc cacgttgtcg cccacgttgt cggcgatcac cgcggggttg cgcgggtcat
     2221 cctcgggaat gccggcctcc accttgccga ccaggtcggc gccgacgtcg gcgcccttgg
     2281 tgaagatgcc gccgcccagt cgcgcaaaga tcgagatcag cgagccgccg aaggccaggc
     2341 cgaccagcgc gtgcagcgcg gtgtcgcgcg cgccggtcat ctgcagcacg gcgccgtaat
     2401 agccggccac gccgagcagg cccaggccga ccaccagcat gccggtgatg gcgccgccgc
     2461 ggaacgccac gtccatcgcc gggcgcatgc cccggcgtgc ggcctcggcg gtgcgtacgt
     2521 tggcgcggac ggacacgttc atgccgatgt agccggc
//
LOCUS       DASZZC010000094         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1100595,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000094 DASZZC010000000
VERSION     DASZZC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100595"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1732)
                     /locus_tag="VHE37_02255"
     CDS             complement(<1..>1732)
                     /locus_tag="VHE37_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34378.1"
                     /translation="KAVYYNAYWQDCHVNLPDQPHAKTCGEFRARRDRGKDFLLNVLP
                     ASPVDAATFNDTWKKWGYSARPDNFDELYTLRYGLNHAPFPNPYPTPTDTPAQVAAGG
                     TGQLPLGLRQLKDASGNWTGQIASAACFQCHGGQIGEPSQIGMENLGLGNNNYDVIMS
                     GQDSSPWADTVVAPLLPSISIDALFNIGIKQRGQNNAVGAFEFLITILDLDSLGINPN
                     PLKTVVGQTGVQDVSHPLAHTQDTPAWWNMGSRPRKFFDAGVSNDSTRIIMAAGPGET
                     STLFSASGLPYRTRIEDYDQDLEAFFLSLKSPSYPGQIDEALAQTGAVLFHTKDLWAL
                     PANANRPKPLGGNGSCASCHGAYSPRYVNDPAYLVDPVMEGVAGHISPLAVIGTDRAR
                     SDMLTPTLRQRWDSTYWGYTDNTPGWVSPEDKNPLVEAADDMYPLSMRPTGVCGWEKE
                     VIGYQAPPLYGVWATAPYFHNGSVPTVEAVLDSSKRAPIWQRRIKTDGMVKGFDQSLD
                     SYDFSALGWKHDDLTCADIPGNMLMNCNPANIEGPSIVELAQNYLNSTLSWAGLITVP
                     DPTPGAVDKRL"
BASE COUNT          265 a          561 c          588 g          318 t
ORIGIN      
        1 ccaggcgctt gtccaccgcg cccggcgtgg gatcgggaac ggtgatcagc ccggcccacg
       61 acagcgtgct gttgaggtag ttctgcgcca gctcgacgat ggacgggccc tcgatgttgg
      121 ccggattgca gttcatcagc atgttgcccg ggatgtcggc gcaggtgagg tcgtcgtgct
      181 tccagcccag cgcgctgaag tcataggaat cgaggctctg gtcgaagcct ttgaccattc
      241 cgtccgtctt gatcctgcgc tgccagatcg gcgcgcgctt ggaagaatcc agcaccgctt
      301 cgacggttgg caccgagccg ttgtggaagt acggcgccgt ggcccacacg ccgtacagcg
      361 gcggcgcctg gtagccgatc acttccttct cccagccgca cacgccggtg gggcgcatcg
      421 acagcgggta catgtcgtcg gccgcctcga ccagcgggtt cttgtcttcc ggcgacaccc
      481 agcccggcgt gttgtcggtg tagccccagt aggtgctgtc ccagcgctgg cgcagcgtgg
      541 gcgtcagcat gtcgctgcgc gcgcggtccg tgccgatgac cgcaagcggc gagatgtgcc
      601 cggccacgcc ttccatcacc gggtcgacca ggtacgcggg atcgttcacg tagcgcggcg
      661 agtacgcgcc atggcacgag gcgcaggagc cgttgccgcc cagcggcttc ggtcgatttg
      721 cattggccgg cagcgcccac aggtccttgg tgtggaacag caccgcgccg gtctgcgcca
      781 gtgcctcgtc gatctggccc gggtagctcg gcgatttcaa agacaggaag aacgcttcca
      841 ggtcctggtc gtagtcctcg atgcgcgtgc ggtacggcag gccgctggcc gagaacaagg
      901 tgctggtctc gcccggcccc gccgccatga tgatgcgcgt gctgtcgttg gagacgccgg
      961 catcgaagaa cttgcgcggg cgcgaaccca tgttccacca cgccggcgtg tcctgcgtgt
     1021 gcgccagcgg gtgcgacacg tcctgcacgc cggtctggcc aacaaccgtc ttgagaggat
     1081 tcggattgat gcccagcgaa tcgagatcga ggatggtgat gaggaactcg aacgcgccca
     1141 ccgcgttgtt ctgcccgcgc tgcttgatgc cgatgttgaa cagcgcgtcg atgctgatcg
     1201 acggcagcag cggcgccacc acggtgtcgg cccacggcga ggaatcctgc cccgacatga
     1261 tcacgtcgta attgttgttg cccaggccca ggttctccat gccgatctgc gacggctcgc
     1321 cgatctgccc gccgtggcac tggaagcacg cagcgctggc gatctggccg gtccagttgc
     1381 cgctggcatc cttcagctgg cgcaggccca gcggcagttg cccggtgccg ccggcggcga
     1441 cctgtgccgg cgtgtcggtc ggcgtcggat acggattggg gaacggcgcg tggttcagtc
     1501 cgtagcgcag cgtgtagagc tcatcgaaat tatcgggccg cgccgaataa ccccacttct
     1561 tccaggtgtc gttgaaggtc gccgcgtcca ccggcgaggc cggcagcacg ttgagcagga
     1621 aatccttgcc gcgatcgcgc cgcgcgcgga actcgccgca ggttttcgca tgcggctggt
     1681 cgggaagatt gacgtggcag tcctgccagt acgcgttgta gtacaccgcc tt
//
LOCUS       DASZZC010000095         9471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_776097,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000095 DASZZC010000000
VERSION     DASZZC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776097"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VHE37_02260"
     CDS             <1..1024
                     /locus_tag="VHE37_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT34379.1"
                     /translation="DEIASDGAGGVTVTGDPQPLGDACTSRDAVQMAGQNIGDLLNAH
                     HVSWGFFQGGFDLSVTNADGSTGCSRTTTSAVTSQKKVDYIPHHEPFQYYASTANPSH
                     ARPSSASNIGRGDAANHQYDIHDFYDALDAGNFPAVSFLKAPGYQDGHAGYSDPLDEQ
                     DFIVSLVNRVQQTPEWGSTAVVIAYDDSDGWYDHQMSPIVNPSAIPAASAGATMNGVA
                     VAGTADALNGDGVCNHSSMQQGNATPATPLGGAQGRCGYGPRLPLLVISPYARSNYID
                     HTLTDQTSILRFIEDNWLHGQRIAGSYDALAGTLDNLFDFSAPSSGPLYLDPATGLPR
                     SCPTCT"
     gene            1074..2867
                     /locus_tag="VHE37_02265"
     CDS             1074..2867
                     /locus_tag="VHE37_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVT34380.1"
                     /translation="MKPCFFRRAWLRCCGLILLSACSHSTVPPGVPSAGGSGAPVRLL
                     IDPANATLAAGESYPYRVNGIYADGSSADVTDRIQWTSADTSIATVDGASVSAVQAGS
                     TTVIAALGSLTAQTPLTVTTFNHVPPPPLPVDFPVKEVDLVAAANPIYGNTEYRNAAS
                     QMFTALFRPYYLATSDDQRAAILARSTYTCAEDLDDPAQPGHLLAFPFDLEPARPGTL
                     VWHRYTCTFTLNYTDAGGSAVSYTSPQYLVNQLDVDYGAYSVEPGVAFQNYDFGGAQP
                     DFHYLGRYVQSLDRMLRRRDDSVPLEFYAGVNGGYDFRIDAWSSSTSDNICPARSSPA
                     DRHSFYSHPAPAQCPMGDDALPSCAAGDQLPYSDDMGDSLVMLAWPERAKSWRDVPFQ
                     SYSCGGLVEGFERGAIALYDQDAYTTRTSANRGDPAELVHGGQRPLSALGAGPLLIRD
                     GHFVYDEAASEEGMPIDNYEIASVTGVGYEHDSGGHLHLHIVVVDGGDNATGLHDWML
                     GPYFMSPYAHAEGALALGNGGDATFWVHPQTPAVAAVLADASQANHGYFHAVFEDSNN
                     RGIVSNCDFSARPLGCRGRPVHDGLFVRARR"
     gene            2909..3223
                     /locus_tag="VHE37_02270"
     CDS             2909..3223
                     /locus_tag="VHE37_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34381.1"
                     /translation="MTTIPRHRGLPTRAMLSPLLLRLMLLPAVLATGGCDLSKLPGNI
                     QQNAPVSAGLPDDKTTGEPQPGRVAPVMHCAPQLATNSVAGVQKSAVIISACGTTGAP
                     RT"
     gene            3220..5301
                     /locus_tag="VHE37_02275"
     CDS             3220..5301
                     /locus_tag="VHE37_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HVT34382.1"
                     /translation="MSQLSRRNFLRLAASAMGAASIPACIRDALAIPAAVGSGTIQDV
                     EHVVILMQENRSFDHYFGLFPGVRGYGDRFPHPGPSGKPVWYQRDAKGQELLPYRLDA
                     SKGNAQRVSGTPHGWPDTHLAWNHGRMGFWPTLKQPQSMSHYSEAELPFQYALANAFT
                     LCDDYHAAVLSSTNPNRLFMFTGTNDPSGFGGGPAVDNTNDDLGDPATGYTWTTYAER
                     LEAAGVSWKVYQDMDDNFTDNSLEGFTSFRQAYNDDPSSPLVVKGLSSTLKNNSLDGL
                     KNDVLAGKLPQVSWIVGPAEYSEHTGPSSPVQGAWYTQQVIEALIADPAVWSKTVLLV
                     MFDENDGMFDHVPPPCAPSRRPDGTLAGASTLDDSTERYHDLKGTAPYSEGWVFGPGM
                     RVPMYVISPWSRGGWVNSQTFDHTSIIRFLENRFGVFEPNISPWRRAMCGDLMSCFNF
                     ADPNDEQFPELPQMTKAEADQIRSDQEALPQIPAPAGADASMPQQPAGLRYSRPLPYE
                     LHASAQVSAGTVTLGFANTGAAGAVFHVYDVLHPGAIPRRYAVEAGKQVSDSWNTADD
                     AGRYDLFVLGPAGFHRRFQGNALPVSAALPEVRVCYDVANGDMCLTLTNSGTADCVFT
                     VTANAYRSDGPWTYTVAAGTQAQPIWPLADSARWYDFSVGCDADASFLRRVAGRVETG
                     KPSFSDPAIGS"
     gene            complement(5309..7624)
                     /locus_tag="VHE37_02280"
     CDS             complement(5309..7624)
                     /locus_tag="VHE37_02280"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HVT34383.1"
                     /translation="MNIHRLSLLGLALGLAACGGSLPGSIYGKTAPDSSSALAIKVLS
                     NRADLISGNDALVEVVPPAGVSAADLAITLDGADIASEFIAGADGHAVGLVTGLKLGA
                     NHLAASGGGQSDAVTIVNHAQGGPVFAGPQLQPWTCRNTDAKDAQCNQDPVISWMYKS
                     SSPAKSGLQSYDPNNPPTDVASTTTDNGTTVPFIVRIETGYQDRDQYAIAALYQPDQA
                     WSAAAPQPQFNHKLLITHGVSCGVDYQTGTAPSVTTFNPIDVTGAGLPAVGGDSVEYA
                     LGAGFAVMSTALDYSGHNCNVALQAESLMMAKEHLIENYGTLRYTIGTGCSGGSLAQQ
                     WIANAYPGIYQGILPTCSFPDAWGTATQFADYHLLLAYFEHPGTWGTGVIWNPEQMAD
                     VEGHVTIVNSLVSDNAQFHVAIPTDTCAGISDAQRYDPNSNPGGVRCDVQDAAINLLG
                     PRPPELWSAQEQQIGHGFAGFPVDNVGVQYGLRSLQNGLITPDQFVDLNLKIGGLDQD
                     TNAIAQRTAAVQPALANAYRSGLINETNNMDQVAIIDCRGPDPGAFHDAYRAFAVRAR
                     LDREHGNHDNQMIWEGPVPIIGDAQCSLNSLQAMDGWLAAVEQDGSSKLLAQKIVADK
                     PSDISDECYDGAGQMLTSSLCPVVAVPGYDDIINVGVVPIYGTPRMVAGDDISTDANK
                     CQLKPLDRSDNYGVLPFSDAQWAQLQALYPDGVCDFSKPGVSQQGTIPWQTYQDDSGA
                     VIYGGTALPAAPAHSGSGWASPAFGVFGGGA"
     gene            7855..8844
                     /locus_tag="VHE37_02285"
     CDS             7855..8844
                     /locus_tag="VHE37_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HVT34384.1"
                     /translation="MEAYLPVALFSALTLSACMAGSMPPAQESSAAAPPPVKFAPFIS
                     GHRGGSGYAPEDTLTAYRNAARLGIDDFETDTVPTKDGVLVLIHDDSVDRTTNCSGKV
                     NSFNYADIIQCDAGYWWTPGQSTTPSTHDDTLPHPFRGKGVVLPRAEDLFAYARSLGP
                     DGPTVTIEIKNGPNEAQYEVACPTTAARLVDLIHQSGIQPRIVVQSFDPTCIATVKQL
                     DPTVQTLYLSEYGALDDLAVCVAGGHDYSAPPYNSVDFTQAYVNLAHAQNVKVDPWTV
                     DTQADLQHMIDLGVDGVITNFPACMLQLQGRPVPANVVAPEGGYSDPAFKVCG"
     gene            8933..>9471
                     /locus_tag="VHE37_02290"
     CDS             8933..>9471
                     /locus_tag="VHE37_02290"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVT34385.1"
                     /translation="MRKITVLAQRTVAAAALGLVALAAHAQAPLQEHATLLAAVGEQE
                     PVRFVLTLPYRDPAAVEDFLARLYTPGDALFHQFLSSEEFTERFGPTREQYDALERAA
                     QDNGLTLEQEHAGRTLLSVSAPAATIRRLFGVQMQRYRSDEGKEYVAPDGVALAPSGI
                     AALGAAVAGLDHRPAHHHFR"
BASE COUNT         1525 a         3247 c         3116 g         1583 t
ORIGIN      
        1 tgacgagatc gccagcgacg gcgccggcgg cgttaccgtc accggcgatc cgcagccgct
       61 gggcgatgcc tgcacctcgc gcgacgccgt gcagatggcc ggccagaaca tcggtgacct
      121 gctcaacgcg catcacgtca gctgggggtt cttccagggt ggtttcgacc tgtcggtgac
      181 caacgccgat ggctccactg gctgctcgcg caccaccacc tcggcggtca cctcgcagaa
      241 gaaggtcgat tacatcccgc accacgagcc gttccagtac tacgcatcga cggcgaaccc
      301 gtcgcatgcg cgcccatcgt cggcatcgaa catcggtcgc ggcgacgcgg ccaaccacca
      361 gtacgacatc catgatttct acgacgcgct ggatgccggc aactttccgg cggtgagttt
      421 tctcaaggct ccgggctacc aggacggcca tgcgggttac tccgatccgc tggatgaaca
      481 ggatttcatc gtcagcctgg tcaaccgcgt ccagcagacg ccggagtggg gcagcacggc
      541 agtcgtcatt gcctacgacg attcggacgg ctggtacgac caccagatga gcccgatcgt
      601 caatccgtcg gcgatccccg ccgcgtccgc gggtgcgacc atgaacggcg ttgccgtggc
      661 cggcaccgcc gacgcgctga acggcgacgg cgtatgcaat cactcgagca tgcagcaggg
      721 caatgccacg ccggcgacgc cgctgggcgg cgcgcagggc cgttgcggtt acggtccgcg
      781 cctgccgctg ctggtgatct cgccgtacgc acgcagcaac tacatcgatc acacgctgac
      841 cgaccagacg tcgatcctgc gcttcatcga ggacaactgg ctgcatggcc agcgcattgc
      901 gggttcatac gatgcgctgg ccggtacgct ggacaacctg ttcgatttct cggcgccgtc
      961 cagcggcccg ctgtatctcg atccggccac cggcctgccg cgttcctgcc ccacctgcac
     1021 ctgacgcgtg tgcgggaggc ggcacgtccg cctcccgcat ggctttcctt ccgatgaaac
     1081 cctgtttttt ccggcgcgca tggctgcgct gctgcggcct gatcctgctg tcggcctgca
     1141 gccattccac cgtgccgccc ggcgtgccgt cggcgggcgg cagtggcgcg ccggtcaggc
     1201 tgctgattga tccagccaac gccacgctcg ccgccggcga aagctatccg tatcgggtca
     1261 acggaattta tgccgacggc agcagcgccg atgtcaccga ccgcatccag tggaccagcg
     1321 ccgacacttc gatcgccacg gtcgatggcg caagcgtcag cgcggtgcag gccggcagca
     1381 ccacggtcat cgcggcactc ggcagcctga cggcgcagac gccgctgacc gtgaccacct
     1441 tcaaccatgt tccgccgcca ccgttgccgg tggatttccc ggtgaaggag gtggacctgg
     1501 ttgccgcggc caatcccatt tacggcaata ccgagtaccg caacgcggca agccagatgt
     1561 tcaccgccct gttccggccg tattaccttg ccaccagcga tgaccagcgc gcggcgatcc
     1621 ttgcgcgcag cacctacacc tgcgcggaag atctcgacga tccggcacag ccgggccact
     1681 tgctcgcctt tccattcgac cttgaaccgg cgcggccggg cacgctggtc tggcatcgct
     1741 atacctgcac cttcacgctg aactacaccg atgcgggtgg cagtgccgtg tcgtacacca
     1801 gcccgcaata tctggtgaac cagctcgacg tcgactacgg tgcgtactcg gtcgagcctg
     1861 gcgttgcgtt ccagaactat gatttcggcg gcgcccagcc ggatttccac taccttggcc
     1921 gctacgtgca gtcgctcgac cgtatgctgc gccggcgcga cgacagcgtg ccgctggagt
     1981 tctatgccgg cgtcaacggc ggctacgact tccgcatcga tgcctggagc agcagcacca
     2041 gcgacaacat ctgcccggca cgctcgtcgc cggcagaccg gcactccttc tattcgcatc
     2101 ccgcaccggc gcaatgcccg atgggcgacg acgccctgcc gtcctgcgcc gccggcgacc
     2161 agctgccgta cagcgacgac atgggcgatt cactggtcat gctggcatgg cccgagcgcg
     2221 ccaagtcgtg gcgcgacgtg ccgttccagt cgtacagctg cggcggtttg gtggaaggtt
     2281 tcgagcgcgg cgccatcgcg ctttacgacc aggacgccta caccacgcgc acctcggcca
     2341 accgcggcga cccggcggaa ctggtgcacg gcggccagcg gccgctgtcg gcgctgggcg
     2401 caggtccgct gctgatccgc gacggccact tcgtctacga cgaggcggcg tcggaagaag
     2461 gcatgccgat cgacaactac gagatcgcca gcgtcaccgg cgttggttac gagcacgaca
     2521 gcggcggcca tctgcacctg catatcgtgg tggtcgacgg cggcgacaac gccaccggcc
     2581 tgcacgactg gatgctcggc ccttatttca tgagtccgta cgcgcacgcc gaaggcgcgc
     2641 tggccctggg caacggcggc gatgcgacgt tctgggtgca tccgcagacg cccgcggttg
     2701 ccgcggtgct ggccgacgcg tcgcaggcga accacggcta tttccacgcg gtgttcgagg
     2761 acagcaacaa ccgcggcatc gtcagcaact gcgatttctc ggcgcgcccg ctcggctgcc
     2821 gcggccgccc ggtgcacgac ggactgttcg tgcgcgcccg tcgttgagaa aaattattca
     2881 atcaatcgtc acagaccgga catctgccat gaccaccatt ccgcggcatc gaggcttgcc
     2941 tacacgcgcc atgctcagtc ccttgctgct gcgcctgatg ctgctgccgg cggtgctcgc
     3001 taccggcggc tgcgacctgt ccaagctgcc cggcaacatc cagcagaatg cgccggtcag
     3061 cgccggcctg ccggatgaca agaccaccgg ggagccgcag ccgggccgcg tcgcgccggt
     3121 gatgcattgc gcgccgcagc ttgctacgaa ttccgtagcg ggcgtgcaga agtccgcggt
     3181 gatcatcagc gcctgcggca ccaccggagc gccgcgcaca tgagccagct ctcgcgccgc
     3241 aatttcctgc gcctggcagc ctctgcgatg ggcgccgcct ccatcccagc ctgcatccgc
     3301 gacgcgctgg ccatcccggc ggccgtcggc agcggcacca tccaggacgt cgagcacgtg
     3361 gtcatcctga tgcaggagaa ccgttcgttc gaccactact tcggcttgtt ccccggcgtg
     3421 cgcggctacg gcgaccgctt cccgcacccg ggcccgtcgg gcaagccggt gtggtaccag
     3481 cgcgacgcca aggggcagga actgctgcct taccggctcg acgccagcaa gggcaacgcg
     3541 cagcgcgtgt cgggcacgcc gcacggctgg ccggatacgc acctggcgtg gaaccacggc
     3601 cgcatgggct tctggcccac gctgaagcag ccgcagtcga tgagccacta cagcgaggcc
     3661 gagctgccgt tccagtacgc gctcgcgaat gcatttaccc tgtgcgacga ctaccacgcg
     3721 gcggtgctgt ccagcaccaa tcccaaccgc ctgttcatgt tcaccggcac caacgatccg
     3781 tcgggcttcg gcggcggtcc ggccgtcgac aacaccaatg acgatctcgg cgatcccgcc
     3841 accggctaca cctggaccac ctacgcggag cgcctggagg cggcgggcgt gtcgtggaag
     3901 gtgtaccagg acatggacga caacttcacc gacaactcgc tggaaggctt caccagcttc
     3961 cgccaggcct ataacgacga tccttcctcg ccgctggtgg tgaagggcct gtcgtcgacg
     4021 ctgaagaaca attcgctcga tggcctgaag aacgacgtgc tcgccggcaa gctgccgcag
     4081 gtcagctgga tcgtcggtcc cgcggaatat tccgagcaca ccggtccgtc gtcgccggtg
     4141 cagggcgcct ggtacacgca gcaggtgatc gaagcgctga tcgccgatcc ggcggtgtgg
     4201 agcaagacgg tgctgctggt gatgttcgac gagaacgacg gcatgttcga tcacgtgccg
     4261 ccgccgtgcg cgccctcgcg ccggcccgac ggcacgctgg ccggggcgtc gacgctggac
     4321 gacagcaccg agcgctacca cgacctcaag ggcacggcgc cctattcgga aggctgggtg
     4381 ttcggccccg gcatgcgcgt gccgatgtac gtgatctcgc cgtggagccg cggcggctgg
     4441 gtcaactcgc agaccttcga tcacacctcg atcattcgct tcctggaaaa ccgcttcggc
     4501 gtgttcgagc ccaacatcag cccgtggcgg cgcgccatgt gcggcgatct catgagctgc
     4561 ttcaacttcg ccgatccgaa cgacgagcag tttcccgaac tgccgcagat gaccaaggcc
     4621 gaggccgacc agattcgcag cgaccaggaa gcgctgccgc agattcccgc acccgccggc
     4681 gcagacgcca gcatgccgca gcagccggcg ggcctgcgct actcgcgccc gctgccctac
     4741 gagctgcacg cgagcgcgca ggtgagcgcg ggcacggtca cgctcggctt cgccaacacc
     4801 ggtgcggccg gtgcggtgtt ccatgtgtac gacgtgctgc acccgggcgc gatcccgcgg
     4861 cgttatgcgg tggaagcggg caagcaggtc agcgacagct ggaacaccgc ggacgacgcc
     4921 ggccgctatg acctgttcgt gcttggcccc gctggctttc accgccgctt ccagggcaat
     4981 gcgctgccgg tgtccgccgc actgcccgaa gtgcgcgtgt gctacgacgt ggccaatggc
     5041 gacatgtgcc tgacgctcac caacagcggc actgcggatt gcgtgttcac ggtgacggcc
     5101 aacgcctacc gcagcgacgg cccgtggacc tacaccgttg ccgccggcac gcaggcgcag
     5161 ccgatctggc cgctggccga cagcgcgcgc tggtacgact tcagcgtggg ctgcgacgcc
     5221 gacgcctcgt tcctgcgccg cgtggccggc cgcgtggaaa ccggcaagcc ctcgttcagc
     5281 gatcctgcga tcggcagctg agcggcgctt acgcgccgcc gccgaacacg ccgaacgccg
     5341 ggctggccca gccgctgccc gaatgcgcgg gcgctgccgg cagcgccgtg ccgccgtaga
     5401 tcaccgcgcc gctgtcatcc tggtaggtct gccagggaat ggtgccctgc tggctcacgc
     5461 cgggcttgct gaagtcgcac acgccgtcgg gatacagcgc ctgcagctgc gcccattgcg
     5521 cgtcggagaa cggcagcacg ccgtagttgt cgctgcggtc cagcggcttg agctggcact
     5581 tgttggcatc ggtgctgatg tcatcgccgg ccaccatgcg cggcgtgccg tagatcggta
     5641 ccacgccgac gttgatgatg tcgtcgtagc cgggcaccgc aaccaccggg cacaggctgg
     5701 aggtgagcat ctgcccggca ccgtcgtagc actcgtcgga aatgtccgac ggcttgtcgg
     5761 cgacgatttt ctgcgccagc agcttgctgc tgccgtcctg ttccaccgcg gccagccatc
     5821 cgtccatcgc ctgcaggctg ttcaggctgc attgcgcatc gccgatgatc ggcaccgggc
     5881 cttcccagat catctggttg tcgtggttgc cgtgctcgcg gtcgaggcgc gcgcgaaccg
     5941 cgaaggcgcg ataggcgtca tggaacgccc ccggatcagg cccgcggcaa tcgatgatcg
     6001 ccacctggtc catgttgttg gtctcgttga tcaggccgct gcggtaggca ttggccagcg
     6061 ccggctgcac ggccgcggtg cgctgcgcga tcgcgttggt gtcctggtca aggccgccga
     6121 tcttgaggtt gagatcgacg aactggtccg gcgtgatcag tccgttctgc agcgagcgca
     6181 ggccgtactg cacgccgacg ttgtcgaccg ggaagccggc aaagccgtgg ccgatctgct
     6241 gttcctgcgc gctccacaat tccggcggac gtgggccgag caggttgatg gccgcgtcct
     6301 gcacgtcgca gcgcacgccg ccgggattgc tgttgggatc gtagcgctgc gcgtcgctga
     6361 tgccggcgca ggtgtcggtg ggaatggcga catggaactg cgcgttgtcg gagaccagcg
     6421 agttgacgat ggtgacgtgg ccttccacgt cggccatctg ctcgggattc cagatgacgc
     6481 cggtgcccca ggtgcccggg tgttcgaagt acgccagcag caggtggtag tcggcgaact
     6541 gcgtggccgt gccccaggcg tcggggaacg agcaggtggg caggatgccc tggtagatgc
     6601 ccggataggc gttggcgatc cactgctgcg ccagcgagcc gccggagcag ccggtgccga
     6661 tggtgtagcg cagcgtgccg tagttctcga tcaggtgttc cttggccatc atcagtgact
     6721 cggcctgcag cgcgacgttg cagttgtggc cggaataatc cagcgcggtc gacatgacgg
     6781 cgaaaccggc gcccagcgcg tactcgacgc tgtcgccgcc gacggccggc aggccggcgc
     6841 cggtcacgtc gatcggattg aacgtggtca cgctcggcgc ggtgccggtc tggtagtcca
     6901 cgccgcacga cacgccatgg gtgatcagca gcttgtggtt gaactgcggc tgcggcgccg
     6961 cggcgctcca cgcctggtcg ggctggtaca gcgcggcgat ggcgtactgg tcgcggtcct
     7021 ggtagccggt ttcgatgcgc acgatgaacg gcacggtggt gccgttgtcg gtggtggtgc
     7081 tggccacgtc ggtcggcggg ttgttcgggt cgtagctctg cagcccgctc ttggccgggc
     7141 tgctcgactt gtacatccag ctgatgaccg ggtcctggtt gcactgcgcg tccttggcgt
     7201 cggtgttgcg gcaggtccac ggctgcagct gcgggcccgc gaacaccggg ccgccctgcg
     7261 catgattgac gatggtgacc gcgtccgact gtccgccgcc ggacgccgcg agatgattgg
     7321 cgccaagctt gagtccggtg accaggccca ccgcgtggcc gtcggcgccg gcgatgaatt
     7381 cgctggcgat gtcggcgccg tcgagcgtga tggcgagatc agcggcgctc acgccggccg
     7441 gcggcacgac ttccaccagc gcgtcgttgc cgctgatcag gtcggcgcgg ttggacagca
     7501 ccttgatcgc gagggcgctg ctgctgtccg gcgcggtctt gccgtaaatg ctgccgggca
     7561 ggctgccgcc gcaggcggcg aggccgagcg cgaggcccag cagggaaagg cggtggatgt
     7621 tcatgtctcc tcgggtcggc actgttggcg gccgattcat attgcaggcg ggcgcaaaaa
     7681 ttatgcggtg cggcggcagc aacaacaaat agtgtgaaat cactccctac cgcacggccg
     7741 ctttgcatgt tataaaatat tagtcatagt taaatttata caataattta taacgtcata
     7801 aatcggcatg cggtgatggg gaaaatacgc gcttcactcg ccccgagatt cttcgtggaa
     7861 gcatatttgc ccgtcgcgct gttcagtgca ctcacgctct ccgcctgcat ggccggctcg
     7921 atgccgccgg cgcaggagtc gtctgcggcc gcgccgccgc cggtcaaatt cgctcctttc
     7981 atctccggtc accgcggcgg ctcgggctac gcacccgaag acaccctgac cgcgtaccgc
     8041 aatgccgcgc gcctgggcat cgacgatttc gagaccgaca ccgtgccgac caaggacggc
     8101 gtactggtgc tgatccacga tgacagcgtc gaccgcacca ccaattgcag cggcaaggtg
     8161 aacagcttca actacgccga catcatccag tgcgacgccg gctactggtg gacgccgggc
     8221 cagtcgacca cgccaagcac gcacgacgac acgctgccgc acccgttccg cggcaaggga
     8281 gtggtgctgc ccagggcgga agacctgttc gcgtacgcca ggagcctcgg ccccgatgga
     8341 ccgaccgtca ccatcgagat caagaacggt ccgaacgagg cgcagtacga agtggcctgc
     8401 ccgaccacgg cggccaggct ggtcgacctg atccaccagt ccggcatcca gccgcgcatc
     8461 gtggtgcagt cgttcgaccc cacctgcatc gccacggtga agcagctcga tccgacggtg
     8521 cagaccttgt acctgtcgga atacggtgcg ctcgacgatc tcgcggtgtg cgtcgccggc
     8581 ggccacgact attccgcgcc gccgtacaac tcggtggatt tcacccaggc gtatgtcaac
     8641 ctggcgcacg cgcagaatgt gaaggtcgac ccgtggacgg tcgacacgca ggccgacctg
     8701 cagcacatga tcgacctggg cgtcgatggc gtcatcacca acttccccgc ctgcatgctg
     8761 cagctgcagg gccggccggt gccggcgaac gtcgtcgcgc cggaaggcgg ctacagcgat
     8821 cccgcgttca aggtctgcgg ctgaaacgcg accggcgtct cagccgggcg gaccggcgcg
     8881 gcgcgcggca gccgagcttg ggctagagtg cagacatcat ccgggggatg tcatgcgcaa
     8941 gatcactgta ctggcacaac gcaccgtggc ggccgcggca ctgggtctcg tcgcactcgc
     9001 ggcgcacgcg caggcgccgc tgcaggagca cgccaccttg cttgccgccg tgggcgagca
     9061 ggagccggtg cgcttcgtgc tcaccctgcc gtaccgcgat ccggcggcgg tggaggattt
     9121 cctggcgcgg ctgtacacgc cgggcgatgc actgttccac cagttcctca gttcggagga
     9181 attcaccgag cgcttcggcc ccacccgcga gcagtacgac gcgctggagc gcgcggcgca
     9241 ggacaacggc ctgacgctgg agcaggaaca cgccggccgc acgctgctgt cggtgtccgc
     9301 gccggcagcg accatccgcc gcctgttcgg cgtgcagatg cagcgctacc gttctgacga
     9361 gggcaaggag tacgtggcgc cggacggcgt ggcgctcgcg ccttcaggca tcgccgcgct
     9421 gggcgccgcc gtggccggtc ttgatcaccg ccccgcgcat catcatttcc g
//
LOCUS       DASZZC010000096         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_265368,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000096 DASZZC010000000
VERSION     DASZZC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265368"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..>2425)
                     /gene="glgP"
                     /locus_tag="VHE37_02295"
     CDS             complement(51..>2425)
                     /gene="glgP"
                     /locus_tag="VHE37_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HVT34386.1"
                     /translation="RAARDRDFLEDYNRALSSFDSYMTAGERPDIAEHLDLQQDLVAY
                     FCAEFGLHESFPIYSGGLGILAGDHCKAASDAGLPFVAVGLLYREGYFTQTIDRDGRQ
                     QAHYTPADFGALPVTLALNGEGRERRVQVRVGEREVQLRIWVAQVGHAPLVLLDSDVP
                     ENNPAERAITHRLYGGDSDMRIQQEIVLGIGGVRALRALGQQPSVFHINEGHAAFLIF
                     ERMRELTSAGTSFDAALEQVAAATVFTTHTPVPAGHDIFNAGQLRWFLPGLAGQLKTS
                     ESQLLALGADAHGGDRFNMTALALRGSRFRNGVSRIHGGVARRMEHYIWPELAPEENP
                     VGHVTNGVHMHTFIGQPWRQLFHDLFRGWRGQLLDTAYWRCIDQIPDERFIAVRRQMK
                     RDLFSDLGERLRRQLRRNAVPEALIRRATRHVDDVNSKTLVLGFARRFATYKRALLIL
                     QDPQRLARMLGDPEWPALLVFAGKAHPRDQPGQQLIQRLYQLSMSEEFIGRLIMIEGY
                     DMLLARNLVQGCDVWLNTPEFPLEASGTSGQKAGINGTVNVSVLDGWWAEGYDAGDGG
                     NGFAIAPVDPRYFGGANDEHARAQRDREEGRQLLDILEHEVIPTYYGTRNQGYAEGWL
                     RIARNSMRTLIPRYTSARMLMDYVRGSYGPAARHGRRLARDGAAERYAQWKSRVLEAW
                     PGVRVALAPALPSVLVHGQPLQLEAEVSGNGLHAQDLAVECLLGHAADDGSFKPLRAV
                     PLLPRGERHGKLLYGCEVEPLPGTQQLRVRARPAHADMAHPLELGRVRWA"
BASE COUNT          361 a          843 c          844 g          377 t
ORIGIN      
        1 gccaccggcc gccagaccgc aagcgaaatg atctggcccg cgcgccgccc tcacgcccag
       61 cgcacgcgcc ccagttccag cggatgcgcc atgtccgcat gcgccggcct tgcgcggacg
      121 cgcagctgct gcgtgccggg caagggctcg acctcgcagc cgtagagcag cttgccgtgc
      181 cgctcgccgc gcggcagcag cggcactgcc cgcagcggtt tgaagctgcc gtcgtcggcc
      241 gcgtggccga gcaggcattc gacggcgaga tcctgcgcat gcaatccatt gccgctcact
      301 tccgcctcca gctgcagcgg ctggccgtgc accagcactg acggcaatgc gggtgccagc
      361 gcgacgcgca cgccgggcca tgcttcgagc acgcgcgact tccactgcgc gtaacgctct
      421 gcggcgccgt cgcgcgcgag ccggcggccg tggcgcgcgg ccgggccgta gctgccgcgc
      481 acgtagtcca tcagcatgcg cgcgctggtg tagcgcggga tcagcgtgcg catggaattg
      541 cgcgcgatgc gcaaccagcc ttccgcataa ccctggttgc gcgtgccgta ataggtggga
      601 atcacctcgt gctcgaggat gtcgagcagc tggcggcctt cctcgcggtc gcgctgcgcg
      661 cgcgcgtgct cgtcgttggc gccgccgaag tagcgcggat cgaccggcgc gatggcaaag
      721 ccgttgccgc cgtctccggc gtcgtagccc tcggcccacc agccatcgag caccgagaca
      781 ttcaccgtgc cgttgatgcc ggccttctgc ccgctggtgc cgctggcctc gagcggaaac
      841 tccggcgtgt tcagccacac gtcgcagccc tgcaccaggt tgcgcgccag cagcatgtcg
      901 tagccttcga tcatgatcag ccggccgatg aactcctcgc tcatcgacag ctggtacagg
      961 cgctggatca gctgctggcc cggctggtcg cgcggatgcg ccttgccggc gaacaccagc
     1021 agcgccggcc attccgggtc gccgagcatg cgcgccagcc gctgcgggtc ctgcaggatc
     1081 agcagtgcgc gcttgtaggt ggcgaagcgc cgcgcgaagc cgagcaccag cgttttcgaa
     1141 ttgacgtcgt cgacatggcg cgtggcgcgg cggatcagcg cctcgggcac ggcgttgcgg
     1201 cgcagctggc ggcgcaggcg ctcgccgaga tcggagaaca ggtcgcgctt catctgacgg
     1261 cgcacggcga tgaatctctc atcggggatc tggtcgatgc agcgccagta ggcggtgtcg
     1321 agcagctggc cgcgccaacc gcggaacagg tcatggaaca gctggcgcca cggctggccg
     1381 atgaaggtgt gcatgtgcac gccgttggtg acatggccga ccgggttttc ctccggcgcc
     1441 agttccggcc agatgtagtg ctccatgcgg cgggcgacgc cgccgtggat gcgcgacacg
     1501 ccgttgcgga agcggctgcc gcgcagcgcc agcgcggtca tgttgaagcg gtcgccacca
     1561 tgcgcgtcgg cgcccagcgc cagcagctgc gattcgctgg ttttcagctg ccccgccagc
     1621 cccggcagga accagcgcag ctggccggcg ttgaagatgt cgtggccggc cggcaccggc
     1681 gtgtgcgtgg tgaatacggt ggcggctgcg acctgctcca gcgcggcgtc gaagctggtg
     1741 cctgcggaag tcagttcgcg catgcgctcg aagatcagga acgccgcgtg tccttcgttg
     1801 atgtggaaca cgctcggctg ctggcccagc gcgcgcagcg cacgcacgcc gccgatgccc
     1861 agcacgatct cctgctggat gcgcatgtcg ctgtcaccgc cgtacaggcg atgggtgatg
     1921 gcgcgctcgg ccgggttatt ttccggcacg tcggaatcga gcagcaccag cggcgcatgt
     1981 ccaacctgcg ccacccagat gcgcagctgc acctcgcgct cgcccacgcg cacctgcacg
     2041 cgccgctcgc ggccctcgcc gttcagcgcc agcgtcaccg gcagtgcgcc gaaatctgcc
     2101 ggcgtgtaat gcgcctgctg gcggccgtca cggtcgatgg tctgggtgaa ataaccctcg
     2161 cgatacagca ggcccaccgc cacgaacggc aggccggcgt cgctggctgc cttgcaatga
     2221 tcgccggcca ggatgcccag gccgccggag tagatgggga aactttcgtg caggccgaac
     2281 tcggcgcaga aatacgccac caggtcctgc tgcaggtcga gatgctcggc gatgtcgggc
     2341 cgctcgccgg cggtcatgta ggagtcgaac gaggacagcg cgcggttgta gtcctcgagg
     2401 aaatcgcggt cgcgcgcggc gcgct
//
LOCUS       DASZZC010000097         9005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1161969,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000097 DASZZC010000000
VERSION     DASZZC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161969"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1882)
                     /locus_tag="VHE37_02300"
     CDS             complement(17..1882)
                     /locus_tag="VHE37_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HVT34387.1"
                     /translation="MTQEFMQRHPRLTELFGIDLRSLALFRAVLGSVVLIVLLRHYCD
                     LTAFYSDYGVTPRAWIIDSDSYNRISLYFLNGSTWFAALLLTVQCVFALMFTLGWHTR
                     LASIVTFFLWGSLMNRAPIVLIGGDILACCLLFWGMFLPLGARWSVDAALAQNPPPRE
                     NQHVSWASAALLLQVVSVYFFSAMLKNGAEWYPDFTGVYYALSLDRYATPLGLWLLNF
                     PQLLKGLSFYVFWLEAFGPLLVFIPFFNRPVRFLMMLCFATMHIGFLLCLELGHFPYV
                     SISSHTVLLGGWFWDWMSRRHQQKDPGTLRIYYDRDCGFCQKTVLLMQQFLVLPRAQV
                     APAQDTPRAKTLLEANYSWVVIDTDDQAYLKWPAFVVLIKHSPLFGWLWRAARLPAFE
                     RPGNAVYDFVGRNRGGFASVSAWLLPMREVRWEPNWYWRQLAGVFLVAVFIWNWCTVE
                     RSLQRYFGPTPALQRVADRTIDALGLPFRMLRIDQLWNMFAPFPLKEDGWMVIPATLA
                     DGSEIDLMHPERKTIDYSKPYHLSQLHENIRWNTYRGRLYENDYTQYRLYYGKYLCRT
                     WNRDKLEQNQDKRLMTFKLVYMVERTPPPGEPTQVEQQTLWKHECFPSASTPPAK"
     gene            complement(1978..2700)
                     /locus_tag="VHE37_02305"
     CDS             complement(1978..2700)
                     /locus_tag="VHE37_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34388.1"
                     /translation="MYRIMTAAAVGVALGTAMLASAASRDESQPEARAYMNLSFGGNA
                     ALPKNFHYGMRLDYDHRFVSHPMPPMLQMDFTDNGFSDARFNGLPFVRRYYLNQAEGA
                     VAAGAAGAGTASTVTVVDWTLLAAGAAGLGVAGYEVSSNSKSDTAPATSGTSTGGGSN
                     TGNSTGGSTTGGSTTGGSTTGGSTTGGSTTGGSTTGGSTTGGLLGFAEYGPQAEATRH
                     TAEYQEWLDGGTGQMGDLAPVK"
     gene            complement(2874..3584)
                     /locus_tag="VHE37_02310"
     CDS             complement(2874..3584)
                     /locus_tag="VHE37_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34389.1"
                     /translation="MQKIMTAVCLGTMMGAAMLAAAAGPADSRPETRAYFSYGFGAHQ
                     LPSNFHYGLRLDQDSRYAAPGTPAVMQVDMSGDGTLNARLNGVNLLSRNFSANQEEGA
                     TAAAATSYSVADWGLIVLGAAGVGYAGYEVSKSHKDDSSSSSTTGTSTGGGSTTGNST
                     TGGSTTGGLLGGGVLGGGSTTGGSTTGGSTTGGSTTGGVLGLAEYGAQSEASYHTRDY
                     QEWLDGGTGQMGDLRSAH"
     gene            3673..4335
                     /locus_tag="VHE37_02315"
     CDS             3673..4335
                     /locus_tag="VHE37_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT34390.1"
                     /translation="MHPSPFPAAGATVTQLVRGPAGDIECLLASPRRPSADAGCALIC
                     HPHPLMGGAMSNKVVYTLAAAALDCGLETVRFNFRGVGASAGVHDHGNGETADTEFLA
                     GWLREKLPGHRLVLAGFSFGAHVSLRAAAQVRPDMQISIAPPLSGKYAASGRPARPQA
                     PWLVVHGRDDEVVSYDETVAALSVYEPPPELVALDGVGHFFHGRLTELRGIVRDFIGR
                     HG"
     gene            4422..5909
                     /locus_tag="VHE37_02320"
     CDS             4422..5909
                     /locus_tag="VHE37_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT34391.1"
                     /translation="MNLFRTKPVEREAEGSGLRRELSAFDLTMLGIGAIIGAGIFVLT
                     GVSAATQSGPAIVLSFIVAGTGCACAALAYAELAASIGGCGSAYGYGYAGFGEFPGWI
                     IGWMLIAEYVISVSAVSVGWSGYFNTALQSMGFGLPDSLLHGPFDATPGLVNLPAAGL
                     VVLLTFLLATGAKMSARANTVMVIIKLVAVALFIGVAMFHVDTSNWHPFIPPQEAVAG
                     SPAGFDWGMKLVDAIRLWLGFDVGGATRFGVPGIFGGGAIIFFAYLGFDAVSTSAEET
                     RNPQRDLPIGIIGSLVICTTLYIIVSGLLTGIVNYRELNVPSPVAYALLRIHQNWAAG
                     VISLGAIAGLTTVCLVMYYGATRLIYAISRDGLLPRGLATLSETSRSPVRIIVISGAV
                     MAFLSGFVSLNNLASITNFATLAAFVVVCAGVVLMRKRQPDLVRPFKTPGFPIVPWIG
                     VLICGYLMLNLSVLTWAWFGGWMVVGLVIYFGYSHTHSVLAGRAP"
     gene            5906..7549
                     /locus_tag="VHE37_02325"
     CDS             5906..7549
                     /locus_tag="VHE37_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HVT34392.1"
                     /translation="MTEQIFAVIDAINGVMLSVPMLLFLLATGLIFTVWSRFCQYRSL
                     THGIALVAGKGIDTSHGPGALTHFQALTAAMSGTVGLGAIAGMAIAVDFGGPGAVFWM
                     WVVGVVGMALKSTEVTLALLYRDTSEHGNPHGGAMYVLREGLGWGRTGAVLGSFFALS
                     MLLFAFTGGNMFQTWSVADTTREYFGVPSWLSGVILAVACGIVILGGIRRIGGVTSLL
                     VPLKCGLYVLAGLYVIVLHREQLPELFRQIFAGAFSRTEGVGAFQGGVIGTAFMWGMK
                     RALFSSESGLGTAPIAHSAVKTPEPVTEGVVAGLEPFIDTIFVCTVTGLVVLSTGVWN
                     RAPVAHWDATPAFVQSADGQWLPQLAQLPADAPPELRGTAAPVFVIADIDGARKRVYG
                     SATAGAITWQPVPAQMAPRVIEPGVFADYRGATLVAKAFDSVHDGLGRWLITAAVWVF
                     GLSCIISYGYYGEQGIIYLVGEKFVMPYRVVWCIAAAAACSGFIRTTVQLDSISTIGM
                     GFMYAINLPLMLLLGRRAMTAYHDYFRRLHNGQIKRPSP"
     gene            7552..8505
                     /gene="hemH"
                     /locus_tag="VHE37_02330"
     CDS             7552..8505
                     /gene="hemH"
                     /locus_tag="VHE37_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HVT34393.1"
                     /translation="MKTGVLLMNLGTPSAPTPAAVRRYLREFLSDRNVVRLPRLLWYP
                     VLYGFILPFRPARLAHAYRSIWTERGSPLLAVSQDQLHSLRALLGERSACALAMTYGE
                     PGVPEALAQLRARQVSRVVLLPLYPQFSGTTTTAALEAVQAALDRSSWQPELVVVNDY
                     HNNAEYIAALAESVRLHWQQHGQGHLLVSFHSIPQRYVAAGDPYQAQCEMTASMLVQA
                     LGLAPDRWSIAYQSRLGREPWLQPYTDQELPRLAAAGVREIDVICPGFSADCLETLEE
                     VAIRYRQLFLASGGTRLRYIPALNAQPQHMRMLAGLVQSRL"
     gene            complement(8522..>9005)
                     /locus_tag="VHE37_02335"
     CDS             complement(8522..>9005)
                     /locus_tag="VHE37_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT34394.1"
                     /translation="QDDQRALLDEIDRREREREEAEARATGAAAVASTAPAAEQPVLN
                     ARDPNLFSEVLFAQGKSDFSEGDVHSLRDALPQLVSLMQRAPKMRVEVVGYADPKTEH
                     ANADKLAAARALSVHAYLIRNGISPKRSARSSQGASRPVVLRGDSSRNRRVEILVYAE
                     "
BASE COUNT         1439 a         2893 c         2993 g         1680 t
ORIGIN      
        1 agctccaccg gcgatttcac ttggccggcg gagtgctggc actcgggaag cactcgtgct
       61 tccacagggt ctgctgctcg acctgggtcg gttcacccgg cggcggcgtg cgctcgacca
      121 tgtagaccag cttgaaggtc atcagtcgct tgtcctggtt ctgctcgagc ttgtcgcggt
      181 tccaggtgcg gcacaggtac ttgccgtagt acaggcgata ttgcgtgtag tcattctcgt
      241 agagccggcc gcggtaggtg ttccagcgga tgttctcgtg cagctgcgac aggtggtacg
      301 gcttgctgta gtcgatggtc ttgcgctcgg gatgcatcag gtcgatctcg ctgccgtcgg
      361 ccagcgtggc gggtatcacc atccagccgt cttccttcag cgggaacggc gcgaacatgt
      421 tccacaactg gtcgatgcgc agcatgcgga agggcaggcc cagcgcgtcg atggtgcggt
      481 cggcgacgcg ctgcaatgcc ggtgtggggc cgaagtagcg ctgcagcgag cgctccaccg
      541 tgcaccagtt ccagatgaag accgcgacca ggaacacgcc ggccagctgg cgccagtacc
      601 agttgggttc ccagcgcacc tcgcgcatcg gcaggagcca ggcgctgacg ctggcgaaac
      661 cgccgcgatt gcggccgacg aaatcgtaga ccgcgttgcc gggccgctcg aacgcgggca
      721 ggcgcgccgc gcgccacagc cagccgaaca gcggcgagtg cttgatcagc accacgaagg
      781 ccggccactt gagataggcc tggtcgtcgg tgtcgatgac cacccaggaa taattcgctt
      841 cgagcagggt cttcgcgcgc ggcgtgtcct gcgccggcgc cacctgcgcg cgcggcagca
      901 ccaggaactg ctgcatcagc agcaccgtct tctggcagaa gccgcagtcg cggtcgtagt
      961 agatgcgcag cgtgcccgga tccttctgct ggtggcgccg cgacatccag tcccagaacc
     1021 agccgccgag cagcaccgtg tgcgaggaaa tgctcacata gggaaagtgg cccagttcca
     1081 ggcacagcag gaagccgatg tgcatggtgg cgaagcacag catcatcagg aagcgcaccg
     1141 gcctgttgaa gaacgggatg aacaccagca gcggtccgaa cgcttccagc cagaacacgt
     1201 agaacgaaag acccttgagc agctgcggga agttgagcag ccacaggccc agcggcgtgg
     1261 cgtagcggtc cagcgacagc gcgtagtaca ccccggtgaa gtccgggtac cactcggcgc
     1321 cgttcttcag catggcgctg aagaaataga ccgacaccac ctgcagcagc agcgcggcgc
     1381 tggcccagga cacgtgctgg ttctcgcgcg gcggcgggtt ctgcgccagc gccgcatcca
     1441 ccgaccagcg cgcgcccagc ggcaggaaca tgccccagaa caacagacag caggccagga
     1501 tgtcgccgcc gatcagcacg atcggcgccc ggttcatcag cgagccccac aggaaaaagg
     1561 tgacgatgct ggccaggcgt gtgtgccagc ccagcgtgaa catcagcgca aagacgcact
     1621 ggaccgtgag cagcagcgcg gcgaaccagg tcgagccgtt gaggaagtac agcgagatgc
     1681 ggttgtagct gtcgctgtcg atgatccacg cgcgcggcgt cacgccgtag tcgctgtaga
     1741 aggcggtgag atcgcagtag tggcgcagca gcacgatcag caccacgctg ccgagcacgg
     1801 cgcggaacag cgccagggaa cgcagatcga tgccgaacag ttcggtcaac ctgggatgac
     1861 gctgcatgaa ttcctgggtc atggacggcg atgttcggat gatggattgc aaatggaatg
     1921 ccggcacaaa cgaagagggg agcctcgcgg ctcccctcgg ttcaccgcaa ccggatttta
     1981 tttcaccggc gcgagatcgc ccatctggcc ggtgccgcca tcgagccatt cctggtactc
     2041 ggcggtgtgg cgcgtggctt ccgcctgcgg gccatattcg gcaaagccca gcaggccgcc
     2101 ggtggtggag ccgcccgtgg tcgagccgcc ggtcgtgctg ccgcccgtgg tggatccgcc
     2161 ggtggtggag ccacccgtcg tcgacccgcc cgtggtggag ccgccggtcg agttgcccgt
     2221 gttgctgccg ccgccggtgc tggtgccgct ggtcgccggc gccgtgtcgg acttcgagtt
     2281 gctggacact tcgtagccgg ccacgcccag gccagcggcg cccgccgcca gcagcgtcca
     2341 gtcgaccacg gtgacggtgc ttgccgtgcc cgcaccggca gcgcctgctg ccaccgcgcc
     2401 ttctgcctgg ttcaggtagt agcggcgcac aaagggcagg ccgttgaagc gcgcgtcgga
     2461 aaagccgttg tcggtgaaat ccatctgcag catcggcggc atcggatggc tgacgaaacg
     2521 gtggtcatag tccaagcgca tgccgtaatg gaagttcttc ggcagcgccg cattgccgcc
     2581 gaagctcagg ttcatatagg cccgggcctc gggctggctt tcgtcgcggc ttgctgcact
     2641 ggccagcatg gccgtgccca gggcaacgcc gactgccgcg gcggtcatga ttcggtacat
     2701 ggaaaccccc caaattgata tctgatttgc gccagatgaa atggcgtgcg cagagcgctg
     2761 cggtactgtc cgctatttcc tgccccaagg caacggcgcc ggcggggaaa atctacccag
     2821 ccagaaacaa aacggggagc catctggctc cccgtatttg caacctgtac cgatcagtgc
     2881 gcagaccgca gatcgcccat ctggcccgtg ccgccatcga gccattcctg gtaatcgcgg
     2941 gtgtgatagc tggcttccga ctgcgcacca tattcggcaa ggccgagcac gccgcccgtg
     3001 gtcgagccac cggtcgtgct gccaccggtg gtcgagccgc ccgtggtgct gccgccgccc
     3061 agcacgccgc cgccgagcag tccgccggtg gtggagccgc cggtggtgga attgcccgtg
     3121 gtcgagccgc cgccggtgct ggtaccggtg gtgctgctgg acgaggaatc gtccttgtgg
     3181 ctcttggaaa cttcgtaccc tgcatagccg acgccggccg cgccaagcac gatcagcccc
     3241 cagtcagcca cgctgtagct ggtagcggcc gcagccgtgg cgccttcttc ctggttggcg
     3301 gagaaattgc gcgacagcag gttcacgccg ttcaggcgcg cgttgagcgt gccgtcgccg
     3361 ctcatgtcga cctgcatcac cgcgggcgtg ccgggagccg cataacgcga gtcctgatcg
     3421 agccgcaggc cgtaatggaa attgctcggc aactggtgcg ccccgaagcc gtagctgaag
     3481 taggcgcgcg tttccgggcg gctgtccgcc gggcctgcag ccgcagcgag catggctgcg
     3541 cccatcatgg tgcccaggca caccgctgtc atgatttttt gcatggtctc cccacagaaa
     3601 aggtacgaat cgaacgaccg ccgaactata ggcggcgcgg caggccgcag gcaagagcga
     3661 tatcctgctg ccatgcatcc gtcgccattc ccagcggccg gcgcgaccgt gacacagctc
     3721 gtgcgcggac cggccggaga catcgaatgc ctgctggcca gcccgcgtcg cccatccgca
     3781 gatgccggct gcgcgctgat ctgccatccg catccgctga tgggtggcgc catgagcaac
     3841 aaggtggtct atacgctggc tgccgccgcg ctggactgcg gtctggagac cgtgcgattc
     3901 aattttcgcg gcgtcggcgc cagcgccggc gtgcacgatc atggcaacgg cgaaacggcc
     3961 gacaccgagt ttctggccgg gtggctccgc gagaagctgc ccgggcaccg cctcgtcctt
     4021 gcgggatttt cattcggtgc gcatgtcagc ctgcgggcgg ccgcgcaggt ccggcccgat
     4081 atgcagatca gcattgcgcc tccgttgtct ggaaaatacg ccgcatccgg ccggcctgcg
     4141 cgtccgcagg caccgtggct ggtggtgcat gggcgcgatg acgaagtcgt gtcctacgac
     4201 gaaaccgtgg cggcgctcag tgtctacgag ccaccgccgg aactggtcgc gctggacggc
     4261 gtcggccatt tcttccacgg ccggttgacg gaactgcgcg gcatcgtgag ggatttcata
     4321 ggccgccacg gatgagcgcc cgcaggcagg cttgttgcta tgctttccgc gatgatccgc
     4381 aatgaaacga ggcgcgcaca cgcgtccggg agaataatcg aatgaacctg ttccgcacca
     4441 agccggtgga acgcgaggcg gaaggcagcg gactgcgccg cgagctgtcg gccttcgacc
     4501 tcaccatgct cggcatcggc gccatcatcg gcgcgggcat cttcgtgctg accggcgtgt
     4561 cggcagcaac gcagagcggg ccggctatcg tgctgtcctt catcgtggcc ggcaccggct
     4621 gcgcctgtgc ggcgctggcc tatgccgaac tggccgcgtc gatcggcggc tgcggcagcg
     4681 cctatggcta cggctacgcc ggcttcggtg agttcccggg gtggatcatc ggctggatgc
     4741 tgattgccga gtacgtgatc tcagtgtcgg cggtgtcggt cggctggtcc gggtacttca
     4801 acactgcgct gcagtcgatg ggtttcggtt tgccggattc gctgctgcac ggtcccttcg
     4861 acgccacgcc tggcctggtc aatctacccg cggcaggatt ggtggtgctg ctcacgttcc
     4921 tgctcgcgac cggagcaaag atgagcgcgc gcgccaacac ggtcatggta atcatcaagc
     4981 tggtggccgt ggcgctgttc atcggcgtcg caatgtttca tgtcgatacc agcaactggc
     5041 atccattcat tccgccgcag gaagcggttg cgggcagccc ggccggattc gattggggca
     5101 tgaaactggt cgatgccatc cgcctgtggc tgggcttcga tgtcggcggt gccacccgtt
     5161 tcggggtgcc gggcattttc ggcggcggcg ccatcatctt ctttgcttat ctcggcttcg
     5221 atgcggtttc gacctcagcc gaggaaaccc gcaatccgca gcgggacctg ccgatcggca
     5281 tcatcggttc gctggtcatc tgcaccacgc tgtacatcat tgtctccggc ttgctgaccg
     5341 gcatcgttaa ctaccgcgaa ctcaacgtgc cttcgcccgt ggcctacgcg ctgctcagga
     5401 tccatcagaa ctgggcggcg ggcgtcatct cgctgggagc catcgccggt ctcaccacgg
     5461 tctgcctggt gatgtactac ggcgccacgc gcctgatcta cgccatctcg cgcgatggac
     5521 tgctgccgcg cggactggcc acgctttccg aaacatcgcg cagcccggtc cgcatcatcg
     5581 tcatctcggg cgcggtgatg gccttcctct ccggcttcgt ttcgctcaac aacctggcgt
     5641 cgatcaccaa cttcgccacg ctggcggcct tcgtcgtggt ttgcgcaggc gtggtgctga
     5701 tgcgcaagcg ccagcccgat ctggtgcgtc cgttcaagac cccgggtttc ccgatcgtac
     5761 cgtggatcgg cgtgctgatc tgcggctacc tgatgctcaa cctgtcggtg ctgacctggg
     5821 cttggttcgg cggctggatg gtcgtgggcc tggtcattta tttcggctat tcccacacgc
     5881 atagcgtgct ggcgggccgc gcgccgtgac cgaacagatt ttcgcggtga tcgacgccat
     5941 caacggcgtc atgctgtcgg tgccgatgct gttgttcctg ctcgccaccg gcctgatctt
     6001 cacggtctgg agccgcttct gccagtaccg ctcgctgacg cacggcatcg cgctggtggc
     6061 aggcaagggc atcgacactt cgcatggacc gggcgcgctg acgcacttcc aggcgctgac
     6121 cgccgccatg tcgggaacgg tcgggctggg cgcaatcgcc ggcatggcca tcgcggtcga
     6181 ctttggcggg cccggcgcgg tgttctggat gtgggtcgtc ggcgtcgtcg gcatggcgct
     6241 caagtccacc gaggtcacgc tggccttgct gtaccgcgat acctcggagc acggcaatcc
     6301 gcatggcggc gcaatgtacg tgctgcgcga aggactgggc tggggccgca ccggcgccgt
     6361 gctcgggagc ttcttcgcgc tgtcgatgct cctgttcgcc ttcaccggcg gcaacatgtt
     6421 ccagacctgg agcgtggcag acaccacgcg ggaatatttc ggagtgccga gctggcttag
     6481 cggagtcatt cttgccgtgg cctgcggcat cgtcatcctg ggaggcatca ggcgcatcgg
     6541 cggcgtcacc tcgctgctgg tccccctcaa atgcgggctt tacgtgctcg cggggctcta
     6601 cgtgatcgtg cttcatcgtg agcagctgcc ggaactgttc cgccagatct ttgccggggc
     6661 gttttcccgg accgagggtg tcggggcctt tcagggtggc gttatcggta cggcgtttat
     6721 gtggggcatg aagcgggcac tgttctccag tgaatccggg ctcggtacgg cgccgatcgc
     6781 acacagcgcg gtcaaaacgc cggagccggt gaccgaaggc gtggtcgctg ggctcgagcc
     6841 cttcatcgac accatcttcg tctgcacggt aaccggattg gtggtgttgt cgaccggcgt
     6901 gtggaatcgt gcgccagttg ctcattggga tgcgacgcca gccttcgtgc aatccgccga
     6961 cggtcaatgg ctgccgcagc ttgcgcagtt gcccgctgat gcgccgcccg agttgcgcgg
     7021 caccgctgcg ccggtgtttg tcattgccga catcgacggt gcacgcaagc gcgtctatgg
     7081 ttcggcaacg gctggcgcga tcacctggca gccggtgccg gcgcagatgg cgccgcgcgt
     7141 gatcgaaccc ggtgtgttcg ccgactatcg cggtgccacg ctggtggcca aggccttcga
     7201 ctcggtgcac gacggattgg gccgctggct gattaccgcc gccgtctggg tgttcggcct
     7261 gtcctgcatc atctcgtacg ggtattacgg cgagcagggc atcatttacc tggttggcga
     7321 aaaattcgtc atgccctatc gcgtggtttg gtgcatcgcc gccgccgccg cgtgctccgg
     7381 cttcatccgc acgacggtgc agctggattc gatcagcacc atcggcatgg gcttcatgta
     7441 tgccatcaac ctgccgctga tgctgctgct cggacggcgg gcgatgacgg cttaccacga
     7501 ttatttccgc cgcctgcaca acgggcagat caagcgcccg tcaccgtagt cgtgaaaacc
     7561 ggcgttctgc tgatgaacct cgggacaccg tcggcgccga cgccggcggc ggtgcgccgc
     7621 tacctgcgcg aattcctctc ggatcgcaac gtcgtgcgcc tgccgcggct actgtggtac
     7681 ccggtgctct acggattcat tctgccgttc aggccggcgc ggcttgcaca cgcataccgc
     7741 tcgatctgga cggaacgcgg atctcctctg ctggccgttt cgcaggacca gttgcattcc
     7801 ctgcgcgcgc tcctgggcga gcgatctgcc tgcgcgctcg ccatgaccta tggcgagccg
     7861 ggcgttccgg aagcattggc gcagctgcgt gcgcggcagg tgagccgtgt cgtgctgctg
     7921 ccgctatacc cgcagttttc tggcacgacg acgactgctg cgctcgaagc ggtgcaggcc
     7981 gcgctggatc gctccagttg gcagccggag ctggtcgttg tcaatgatta ccacaacaat
     8041 gccgagtaca ttgcggcgct ggcggaaagc gtgcgcctgc attggcagca gcatggccag
     8101 ggccatttgc tggtctcgtt tcattcgatt ccgcagcgct atgtagcggc cggtgatccc
     8161 taccaggcac agtgcgagat gaccgcttcg atgcttgtac aggcactggg gctggcgccg
     8221 gatcggtggt cgatcgcgta ccaaagccgg ctgggacgtg agccctggct gcagccatac
     8281 accgaccagg aattgccgcg gctggcagca gccggggtgc gcgaaatcga tgtgatctgc
     8341 ccagggttct cggcggattg cctggaaaca ctcgaagaag tggcgatccg ttaccgccag
     8401 ctcttcctgg ccagcggagg cacccgcctc cgctacattc ctgcactcaa cgcccagccg
     8461 cagcacatgc ggatgctcgc cggcctggtg cagtcgcggc tatgaaggcc gcgccggtcg
     8521 atcactccgc gtaaacgagt atttccaccc ggcggttgcg gctgctatcg ccgcgaagca
     8581 cgaccggtcg tgaagcgccc tggctgctgc gcgcgctgcg cttgggtgag atgccgttgc
     8641 ggatcaggta ggcatgcacc gagagggcgc gggcggcggc gagcttgtcg gcgtttgcat
     8701 gctcggtctt ggggtcggca taaccgacga cctccacgcg catcttgggc gcgcgctgca
     8761 tcagcgaaac cagctggggc agggcatcgc gcaggctgtg cacatcgcct tcgctgaaat
     8821 cgctcttgcc ctgggcgaag aggacctcgc tgaacaggtt cggatcgcgc gcgttgagca
     8881 ccggctgctc ggccgcgggt gcagtcgatg cgacagcagc tgcacctgtg gctcgggcct
     8941 cggcttcttc gcgctcgcgt tcacgtcggt cgatttcatc caacaacgcg cgctgatcgt
     9001 cctgc
//
LOCUS       DASZZC010000098         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_843584,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000098 DASZZC010000000
VERSION     DASZZC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843584"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..2396)
                     /locus_tag="VHE37_02340"
     CDS             complement(216..2396)
                     /locus_tag="VHE37_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34395.1"
                     /translation="MKHLLSIGALVLTGFSADVIAADTTPNAFHFTDKTGAQFSTVYK
                     SNKITVKGINAPAPIGVTGGSYSIGCNGTFTEAAGTIMLNQTVCVQQTSAATASTSTD
                     TTLTIGGVSDTYTVTTQAADSTPAQFTLIDKTGVDPGVAVTSTPVAIKQTNTPAAISI
                     TGGTYSINCGADNTYTSASGMVDPGSSVCVRNTSSSTPGAAVVTKLTVGGVSDNFSST
                     TADASATGTLAPFALSNGTIKLADFTKPFGAANPVVIATAVDPGTLQNARSWITGTVS
                     GTTVSNIRTDRLTLVKAGKVIGAKLTAGTVNNTAQISTIADACSIDGHGENIQNTANS
                     WAMIRRAGPDAQCSTSDDSEALVRLNFKGTDAPVLIDGLNRVDGIFNQSTGALVGFIT
                     VEAGPSLVHRDVNMANPVVIMSLSSSNVERMEVLPSKLYYKLTAKGQAHPQLFRYDET
                     TNSTTISPALYTFTQSTGFDGALDNAAYDGSNVYFGDGTNIVRVAHSASDQSTAAIVA
                     TVPDGETISQIALPDFSATRLVISTFNFSGFGGGIYSAVKTATAGTLTVLAQNELTGP
                     TFNNLVIVNQGTVFINRSIANSSASQLRVNVDGTNPVETANAYWAGVSLDTSFDFSAH
                     GTNNGGPPVVALVQATVASGNATLQTVDPSTFAVGLTLGTINSVDPMPTIFGFGFGRY
                     LELVAEINRSGAVDSDVYSVDVQTANSLHALATSVHDDLPIGTH"
     gene            complement(2537..>2771)
                     /locus_tag="VHE37_02345"
     CDS             complement(2537..>2771)
                     /locus_tag="VHE37_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVT34396.1"
                     /translation="ACIGSGAEFPAFAAHVTASEDAWFQLPELDFGLIPGAGGTVSIA
                     RRVGRQRTAWLVLSGKRIDAQRALDWGLVDRVL"
BASE COUNT          484 a          817 c          949 g          521 t
ORIGIN      
        1 gtacccgacg catgcggact actactgcaa gatgaaagcc gccgcggcgc ggcaggtggc
       61 gctgggattc ctgctgccgg ccgaccgcga cgaactgatg gcgcgcgtgg aggccgccag
      121 caaccgctgg cagcaggctg gtacgcgcag ctgcgagtga cgggccaata aaaaggccgc
      181 gccatcgctg gcgcggccca tcgaacaacg accaatcagt gggtgccgat cggcagatcg
      241 tcatgcacgc tggttgccag ggcgtgcagc gaatttgccg tctgcacatc caccgagtag
      301 acgtcgctgt cgacagcgcc gctgcggttg atctcggcca ccagttccag gtagcggcca
      361 aagccgaaac cgaaaatcgt cggcatcgga tccaccgagt tgatcgtgcc cagggtcagg
      421 ccgaccgcga aggtggatgg atccaccgtc tgcagcgtcg cattgcccga ggccaccgtc
      481 gcctggacca gggccacgac cggcgggcca ccattgttgg tgccgtgcgc gctgaaatcg
      541 aagcttgtgt ccagcgaaac gccggcccag taggcattgg cggtctccac cgggttggtg
      601 ccatcgacat tcacccgcag ctgcgacgca ctggagttgg cgatgctgcg attgatgaac
      661 acggtcccct ggttgacgat caccaggttg ttgaaggtgg ggccggtcag ttcgttctgc
      721 gccagcacgg tcagcgtccc ggccgttgcg gttttcaccg cggaatagat gccgccgccg
      781 aaaccagaga agttgaaggt cgagatgacc agccgcgtgg ccgagaagtc aggcagcgcg
      841 atctgcgaaa tggtttcgcc gtcgggaacg gtagccacga tagctgccgt gctctggtca
      901 cttgcgctgt gtgcgacgcg gacgatgttg gtgccatcgc cgaagtagac attgctgccg
      961 tcgtaagccg cattgtcgag tgcgccgtcg aagccggtgc tctgggtgaa ggtgtaaaga
     1021 gccggcgaaa tggtggtgct attggtcgtt tcgtcgtagc ggaacagctg cgggtgtgcc
     1081 tggccctttg cggtgagctt gtaatagagc ttgctgggca acacctccat ccgctccacg
     1141 ttggaactgc tcaggctcat gatgaccacc ggattggcca tgttgacatc gcggtggacc
     1201 agcgaggggc ccgcctccac cgtgatgaac ccaaccagtg cgccggtcga ttgattgaag
     1261 atgccatcga cacgattgag gccgtcgatc agcaccggcg catccgtacc cttgaagttg
     1321 aggcgcacaa gggcttcgct gtcgtcggat gtggagcact gtgcatcggg gccggcgcgc
     1381 cggatcatcg cccaggaatt ggccgtgttc tggatgttct cgccatggcc gtcgatgctg
     1441 caggcatcgg caatcgtcga gatctgcgcg gtgttgttga ccgtgccggc ggtcagcttg
     1501 gcgccgatca ctttgccggc cttgaccagg gtgaggcggt cggtgcggat attgctcacg
     1561 gtagtgccgc tcaccgtgcc ggtgatccag cttcgtgcgt tctgcaacgt gccggggtca
     1621 accgcagtcg cgattaccac cggattggcg gcgccgaatg gcttggtgaa atcagccagc
     1681 ttgatggtgc cgttggacag ggcaaacggt gccagcgtac cggtggccga tgcgtcggcc
     1741 gtggtcgaac tgaaattgtc ggagacaccg cccacggtca gcttggtgac gaccgcggca
     1801 cctggtgtgc tcgacgaggt gttgcgcacg cagacgctgc tgccgggatc gaccatgccg
     1861 ctggcgctgg tataggtgtt gtccgcgccg caattgattg aatacgtgcc gccggtgatg
     1921 ctgatcgccg ccggcgtgtt ggtctgcttg atcgcaaccg gagtggaagt gaccgccacg
     1981 cccggatcaa cgcccgtctt gtcgatcagg gtgaactgcg ccggcgtgga atcggcagcc
     2041 tgtgtggtga cggtataggt gtcggagacg ccgccgatgg tcagggtcgt atcggtcgac
     2101 gtgcttgcgg tggcggccga agtctgctgc acgcacaccg tctggttcag catgatggtg
     2161 ccggccgcct cggtaaaggt gccgttgcag cctatggagt agctgccgcc ggtgacgccg
     2221 atgggcgcgg gcgcgttgat gcccttgacc gtaatcttgt tcgatttgta gaccgtgctg
     2281 aattgggcgc cggtcttgtc ggtgaagtgg aaggcattgg gcgtggtgtc tgctgcaatg
     2341 acatcagcgg agaatccggt gagcaccaag gcgccgatcg agagtaggtg tttcatgagc
     2401 atccccagaa ttggccgcgg gccgggcggc ccgaaaatac ttccctgcag caccggcgag
     2461 gttactccag ccgccagacg gtggcgatgc catccttgaa ctgcgtcacg ggctttacct
     2521 ttcggccacg caactcttac aagacccggt cgaccagacc ccaatcaagc gctcgctgcg
     2581 cgtcgatgcg cttgccggac aggaccagcc aggcggtgcg ctggcggccg acgcggcggg
     2641 cgatggacac ggtgccgccg gcgccgggga tcaggccgaa atcgagttcc ggcagctgga
     2701 accaggcatc ctcgctggca gtgacgtgcg cggcgaacgc cggaaactcc gcgcccgagc
     2761 cgatgcacgc g
//
LOCUS       DASZZC010000099        10043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_907814,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000099 DASZZC010000000
VERSION     DASZZC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907814"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VHE37_02350"
     CDS             <1..768
                     /locus_tag="VHE37_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HVT34397.1"
                     /translation="KGELYQQLDGGVAVINADDVYAPLWRGFAGRASIITFGFDEHAD
                     VRASNAQPHPANSPTGTRFTLHAQEGRVGVTLPLPGRHNVSNALAAAACAVALGMDLA
                     DIAAGLARTEAAAGRVNWRQTPEGARLLDDSYNANPTSLEAGMQLLAGLPGERWLILG
                     SMAELGADAPRLHLEAGKRARELGIERLMGMGTLAAEAVKGFGPGASWFDDAHALADA
                     ARARLSPNVAVLVKGSRSARMERVVAALCGTTVSGGH"
     gene            771..1859
                     /gene="mraY"
                     /locus_tag="VHE37_02355"
     CDS             771..1859
                     /gene="mraY"
                     /locus_tag="VHE37_02355"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HVT34398.1"
                     /translation="MLLWLAEYLKHEISGFNVFTYLTFRMVLGILTALVFSFAFGPLM
                     IERLYRFKFGQPVRDDGPQTHLKKTGTPTMGGVLILCAITVSTLLWANLSNRFVWFSL
                     LVTLAFGAVGFADDYIKIQHKNPKGLPARQKYFWLSLAGFGVSTLIYYTSAPGSMQTT
                     LIAPLFKDVSIQLGIFFIPWAYLVIVGSSNAVNLTDGLDGLAIMPTVLVASALAVFAY
                     STGNVKIAGYLGIPYISGVGEVAIFCTSIAGAGLGFLWFNAYPAQVFMGDVGALALGA
                     ALGVVAVLVRQELVLAIMGGVFVAETFSVALQVLYFKYTGGKRIFRMAPLHHHYELKG
                     WPEPKIIVRFWIVTLILVLIGLSTLKVR"
     gene            1859..3205
                     /gene="murD"
                     /locus_tag="VHE37_02360"
     CDS             1859..3205
                     /gene="murD"
                     /locus_tag="VHE37_02360"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HVT34399.1"
                     /translation="MNRRYQGQTFLVAGLGASGVSAANYLVAQGATVHVTDAAKAPPH
                     AAKLCDKITNEMRFIGQLITAKNEVRYQACVLSPGISPALPFVQQLRDAGTEIIGDIE
                     LFARDANARNSARDANAPVIGITGSNGKSTVTTLVGEMARAAGLRAGVGGNLGTPALE
                     LLRDDAQLYVLELSSFQLETTSSLKCRAAALLNLSQDHLDRHGTMENYGAVKARIFSG
                     CDVAVVNRDDPAVMQLAPRDAITFGLDAPRGDHYGVRDGWLVRGEEKLIATDALKIFG
                     THNWANALASLALAEAAGIERAAAVRALVAFTGLPHRCEFVAEHAGVSYFNDSKGTNV
                     GSTLAALNGLPAPIVWLAGGQGKGQDFSELREPLARKGRAAVLFGEDAARIEHDVYGA
                     VPVYRERDMQRALERARALAQRGDHVLLSPACASFDQFRNYVERGEKFRAAVRALA"
     gene            3202..4356
                     /gene="ftsW"
                     /locus_tag="VHE37_02365"
     CDS             3202..4356
                     /gene="ftsW"
                     /locus_tag="VHE37_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HVT34400.1"
                     /translation="MSAVLSLPLPRARYGLDGWFCAAAAILMGWGLVMVASASVAQAE
                     KMTGAPFYYLYRQLLFMVLGMGLGAVIYCVPMRQWQQSGGWLLMFALMLLALVLVPGI
                     GIKVNSARRWLDLGLFRLQASEPARLALVLYLAGYIARRQIEVQHGWKGLAIPLGLLV
                     LPCLLLLLEPDFGATAVLLAVAFLMLFLGGARLIYFGGLLGSVTLAMSVLAIAAPYRM
                     KRLLNFTDPWADIEHGGWQLAQSLIAVGRGDWAGVGLGNSVQKLLYLPEMHTDFIFAI
                     LAEEFGLIGVALLLALFGILVWRGFAIGREAETQQESFKAYLCYGLSGWLGLQALINM
                     AVNMGVLPTKGLTLPLVSYGGSSLITACVMLALILRVDYENRLALTERPR"
     gene            4353..5417
                     /gene="murG"
                     /locus_tag="VHE37_02370"
     CDS             4353..5417
                     /gene="murG"
                     /locus_tag="VHE37_02370"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HVT34401.1"
                     /translation="MKALIMAGGTGGHVFPALAVAEVLRARGHEVVWMGAPESFEARV
                     VPQQGYRVEFVRVSGLRGKGAMKLLLAPLLLLRALIDALAALRRVRPDFVLGMGGFAS
                     GPGGIAAWLSRKPLLIHEQNAAAGMTNRLLARIATRVLEAFPRTFAGAVTVGNPVRRG
                     FAELPAPEQRLQHDGRARLLVIGGSQGARALNQLVPQALALVPREQRPQLRHQAGRTL
                     ELAQRSYAATDVEVSLEQFINDMPAAFAWADLVICRAGASTIAEISAAGCAALLVPFP
                     AAVDDHQTRNGEYLVRAGAALLLPESTLTPQLLAQTLTALLADRAKLISMARAARTCA
                     WNDAAGRIAQLCLQTAGAAA"
     gene            5414..6847
                     /gene="murC"
                     /locus_tag="VHE37_02375"
     CDS             5414..6847
                     /gene="murC"
                     /locus_tag="VHE37_02375"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HVT34402.1"
                     /translation="MSESALQNLMNAPMRRVRRVHMLGIGGAGMAGIAEVLLNLGYVV
                     SGTDLKDSAATKRLAQLGAKVYFGHATAHAQGADVVVVSSAVREDNPELQYAREHRVP
                     VVRRAEMLAELMRFRYGIAIAGTHGKTTTTSLVASVLAEGGLDPTFVVGGKLKSAGAN
                     ARLGAGAYLVAEADESDASFLHLSPMIAAVTNIDADHLETYGGDFRRLRATFVEFLLR
                     LPFYGLAVLCVDDPVVRETLPEVGRPVLTYGFAGDADVRALEVQAQGAGASFEVQFRD
                     GVREKFQLNLPGRHNVQNALVAVAIARELGVSFDAMRRAFASFEGVGRRCEPHGEIGL
                     PRGKVLVVDDYGHHPREIAATFQAMRAAYPQRRLLVVFQPHRYTRTRDLFEDFCEVLS
                     TADALLLLEVYAAGEDAIEGADGRALARGIRARGRVEPVFVRSAQEVPAALSAIASNG
                     DLVLTLGAGDVGSVPALLEKFSASGAP"
     gene            6844..7734
                     /gene="murB"
                     /locus_tag="VHE37_02380"
     CDS             6844..7734
                     /gene="murB"
                     /locus_tag="VHE37_02380"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HVT34403.1"
                     /translation="MKGVLRTHEPLSRHTSWRVGGPADRYYEPADREDLAQFVRSLPA
                     NEPILWLGLGSNLLVRDGGVRGTVISLHGALDDLRREADDGIYAEAGVHCARLAKFAV
                     AEHFSGAGFLAGVPGTVGGALAMNAGAHGGETWNAVNEAEVMYRDGTTQWLARMQFRY
                     SYRHVELPAGMVGFVAARFRFAPDAGKGAEAEMKSWLARRKATQPVGKPSAGSTFRNP
                     PGDHAARLIEQCGLKGYRIGGAEVSTLHANFILTGDDARAADVEALIAHVRRVVADRT
                     GVALEPEVRIVGEAHASAHH"
     gene            7715..8665
                     /locus_tag="VHE37_02385"
     CDS             7715..8665
                     /locus_tag="VHE37_02385"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HVT34404.1"
                     /translation="MRQRTTDPRAFGRVGVLMGGWSAERQVSLWSGEGVLAALISKGV
                     DVVGIDADRATVLSLGARGIDRVFNVMHGTGGEDGSLQACLELQNIPYTGSGVLASAL
                     AMDKLLTKRIWKAEGLPTPEWLVLHDAQEALRAGEKLGYPYFVKPAAEGSSVGIGKVK
                     FATSAVSAFRRAQGDYDGIDRTVVAEKFVDGPEYTCAILDGEALPIIRIEPDGEFYDY
                     NAKYISDNTRYHCPAGLDAPQEAQIAALCLRAFQLIGAHGWGRVDFMLDPQQQPWLLE
                     VNLVPGMTSHSLVPMAAKARGLGYADLCWALLETTLEGGR"
     gene            8662..9426
                     /locus_tag="VHE37_02390"
     CDS             8662..9426
                     /locus_tag="VHE37_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HVT34405.1"
                     /translation="MNVAAMRLPGMDVNTAKLGPVLAGVAALLCVLIGGWWFGRVHDV
                     RQVRNLEIVGPLKHVSPADIATVLQPHVDSGFLALDLDEARDSLEQLPWVSRARAERY
                     WPAGVRVQVWEREAGARWNERQLLDVDAHAYTPAPSDLVGPLPTLRGPVGHEKEVLDA
                     FQRYSKRVEGTPFELAGIALDARGEWSATTKGGIGLRLGRGSPDARFDVLVTTVSKSL
                     ADRLGQVDYVDLRYTNGFAVGWKAGAQQPAPGAAHG"
     gene            9419..>10043
                     /gene="ftsA"
                     /locus_tag="VHE37_02395"
     CDS             9419..>10043
                     /gene="ftsA"
                     /locus_tag="VHE37_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HVT34406.1"
                     /translation="MAKKDARNLLVGIDIGTSKVVAVIGEVNHDGNIEVVGEGSAPAR
                     GMNRGDVVNIEATVQSIRRAVENAEHMAGCRAQSVYVGISGTHIKSYNSTGVAPVRAR
                     EVSDKDVESVIDAARAIAIPADQKILHVVPQEFVVDGREGIHEPVGMFGVRLEAKVHM
                     VTGSLSAAQNIYKCVESCGLSVDKLILQHLASSYAVLLPDERDLGICR"
BASE COUNT         1539 a         3213 c         3534 g         1757 t
ORIGIN      
        1 aaaggcgagc tgtaccagca gctcgacggc ggcgtggcgg tgatcaacgc cgacgacgtc
       61 tacgcgccgc tgtggcgcgg cttcgccggg cgtgcatcga tcatcacctt tggtttcgat
      121 gagcatgccg acgtgcgcgc gagcaacgcg cagccgcacc cggcgaattc gcccaccggc
      181 acgcgcttca ccctccacgc gcaggaaggc cgtgtgggcg tcacgctgcc gctgccggga
      241 cggcacaacg tgagcaatgc gctggcggcc gcggcctgcg cggtggcgct gggcatggac
      301 ctggccgaca tcgccgccgg ccttgcgcgc accgaggctg cggccgggcg agtcaactgg
      361 agacagacgc cggaaggcgc gcgcctgctc gacgacagct acaacgccaa tcccacctcg
      421 ctggaagccg gcatgcagct gctcgccggc ctgcccggcg agcgctggct gatcctcggc
      481 tcgatggcgg aactcggcgc cgacgcgccg cgcctgcacc tggaagctgg caagcgcgcg
      541 cgcgagctgg gcatcgagcg gctcatgggc atgggcacgc tggcggccga agcggtgaaa
      601 ggttttgggc cgggcgccag ctggttcgac gacgcccatg cactcgccga tgccgcgcgc
      661 gcgcggctgt cgccgaacgt ggcggtgctg gtcaagggat cgcgttccgc gcgcatggaa
      721 cgcgtggtgg ccgcgctgtg cggcacgact gtcagcggag gacactgagc atgctgctgt
      781 ggctggccga atacctgaag cacgagatca gcggcttcaa cgtctttacc tatctcactt
      841 tccgcatggt gctgggcatc ctcaccgcgc tggtgttctc gttcgccttc gggccgctga
      901 tgatcgagcg gctgtaccgc ttcaagttcg gccagccggt gcgcgacgac ggtccgcaga
      961 cgcatctgaa gaaaaccggc acgccgacca tgggcggcgt gctgatcctg tgcgccatca
     1021 ccgtgtcgac cctgctgtgg gccaacctgt ccaaccgctt cgtctggttt tcgctgctgg
     1081 tcacgctggc cttcggggcg gtgggatttg ccgacgacta catcaagatc cagcacaaga
     1141 atccgaaagg cctgccggcg cggcagaaat atttctggct ttcactggcc ggcttcggcg
     1201 tgtcgacgct gatctactac acgtcggcgc cgggctccat gcagaccacg ctgatcgcgc
     1261 cgctgttcaa ggacgtgtcg atccagctcg gcatcttctt catcccgtgg gcctacctgg
     1321 tcatcgtcgg ctcgagcaac gcggtgaacc tgaccgacgg cctcgacggc ctggccatca
     1381 tgccgacggt gctggtggcc tcggcgctgg cggtgttcgc ctacagcacg ggcaacgtga
     1441 agatcgccgg ctacctcggc attccatata tctccggcgt cggcgaagtg gcgatcttct
     1501 gcacgtccat tgccggtgcc ggtctcggct ttctctggtt caacgcctat cccgcgcagg
     1561 tcttcatggg tgacgtgggc gcgctggcgc tcggtgccgc gctcggcgtg gtggcggtgc
     1621 tggtgcggca ggaactggtg ctggcgatca tgggcggcgt gttcgtcgcc gagacttttt
     1681 cagtcgcgct gcaggtgctg tacttcaagt acaccggcgg caagcgcatc ttccgcatgg
     1741 cgccgctgca ccatcactac gagctcaagg gctggcccga gccgaagatc atcgtgcgct
     1801 tctggatcgt cacgctgatc ctggtgctga tcggcctgtc gacgctcaag gtccgttgat
     1861 gaaccggcgc taccagggcc aaaccttcct cgtcgccggg ctcggcgcga gcggggtgtc
     1921 cgcggccaat tacctggtgg cgcagggcgc aaccgtgcat gtgacggacg ctgccaaggc
     1981 tccaccgcat gccgcaaaac tgtgtgataa aataaccaat gaaatgcgat tcattggaca
     2041 attgataaca gcgaaaaacg aggtgcggta ccaggcctgt gtgctctcgc cgggcatttc
     2101 gccggcgctg cctttcgtgc agcagctgcg cgatgcgggc acggaaatca tcggcgacat
     2161 cgagctgttc gcgcgtgacg cgaacgcaag aaactctgcg cgtgatgcga acgcgcccgt
     2221 catcggcatc accggctcca acggcaagag cacggtgacc acgctggtcg gcgagatggc
     2281 gcgcgcggcg ggactgcgcg ccggcgtcgg tggcaatctc ggcacgccgg cgctcgaact
     2341 gctgcgcgac gatgcgcagc tctacgtgct ggagctgtcg agcttccagc tggaaaccac
     2401 ctcgagcctg aaatgccgcg ccgcggcgct gttgaacctt tcgcaggatc acctcgaccg
     2461 ccacggcacc atggaaaatt atggtgcggt gaaggcgcgc attttctccg gctgcgacgt
     2521 ggccgtggtc aatcgcgacg acccagcggt gatgcagctt gcgccgcgcg atgccatcac
     2581 cttcggactg gatgcgccgc gcggcgatca ttacggcgtg cgcgacggct ggctcgtgcg
     2641 tggcgaggag aaactgatcg ccaccgacgc gctgaagatc ttcggcacgc acaactgggc
     2701 caacgcgctg gcgtcgctgg cgctggccga agcggcgggc atcgagcggg ctgccgcagt
     2761 gcgcgcgctg gtcgcgttca ccggcctgcc gcatcgctgc gagttcgtcg ccgagcacgc
     2821 gggcgtgtcg tacttcaacg attcaaaggg caccaacgta ggctccacgc tggctgcgct
     2881 caacggcctg ccggcgccga tcgtgtggct ggccggcggc cagggcaagg gccaggattt
     2941 ctccgagctg cgcgagccgc tggcgcgcaa gggtcgcgcc gccgtcctgt tcggtgaaga
     3001 cgccgcgcgt atcgagcacg atgtgtacgg cgcggtgccg gtgtaccgcg aacgcgacat
     3061 gcagcgcgcg ctggagcgtg cccgtgcgct cgcacagcgt ggtgaccacg tgctgctgtc
     3121 gcccgcctgc gcgagtttcg accagttcag gaattacgtg gagcgcggcg aaaaattccg
     3181 cgccgcggtg agggcgctgg catgagcgct gtcctctccc tgccgctgcc gcgtgcgcgc
     3241 tacggcctcg acggctggtt ctgcgccgcc gccgccatcc tcatgggctg gggcctggtg
     3301 atggtcgcat cggcctcggt ggcgcaagcc gagaagatga ccggcgcacc gttctactac
     3361 ctgtaccgcc agctgctgtt catggtgctc ggcatgggac tgggcgcggt gatctactgc
     3421 gtgccgatgc gccagtggca gcagtcgggc gggtggctgc tgatgttcgc gctgatgctc
     3481 ctcgcgctgg tgctggtccc cggcatcggc atcaaggtga acagcgcgcg gcgctggctc
     3541 gatctcggct tgttccgcct gcaggcctcg gagcctgcgc gcctggcgct ggtgttgtat
     3601 ctcgccggct acatcgcgcg gcggcagatc gaggtgcagc atggctggaa gggcctggcc
     3661 atcccgctcg ggctgttggt cttgccgtgc ctgctgctgc tgctggagcc ggacttcggc
     3721 gccaccgcgg tgttgctggc cgtcgccttc ctgatgctgt tcctgggcgg cgcgcgcctg
     3781 atctacttcg gcggcctgct cggctcggtg acgctggcga tgagcgtgct ggccatcgcc
     3841 gcgccgtatc gcatgaagcg cctgctcaac ttcaccgatc cgtgggccga catcgagcac
     3901 ggtggctggc agctggcgca gtcgctgatc gccgtcggcc gcggcgactg ggccggcgtc
     3961 ggcctgggca acagcgtgca gaaactgctg tatctgccgg agatgcacac cgatttcatc
     4021 ttcgcgattc ttgccgagga attcgggctg atcggcgtgg cgttgctgct cgcgctgttc
     4081 ggcatcctgg tgtggcgcgg cttcgccatc ggccgcgagg cggagacgca gcaggagtcg
     4141 ttcaaggctt atctctgcta cggactgtcc ggctggctcg gcctgcaggc gctgatcaac
     4201 atggcggtca acatgggcgt gctgccgacc aagggcctca ccctgccgct ggtgtcgtac
     4261 ggcggctcgt cgctgatcac cgcctgcgtg atgctggcgc tgatcctgcg cgtggattac
     4321 gagaaccgcc tcgcgctcac ggagcggccg cgatgaaagc gctgatcatg gcgggcggca
     4381 ccggcggaca tgtgttcccc gcgctggcag tggcggaagt gctgcgcgcg cgcggccacg
     4441 aggtggtgtg gatgggcgcg ccggaaagct tcgaggcgcg cgtggtgcct caacaaggtt
     4501 atcgcgtgga gttcgtgcgc gtgtccggcc tgcgcggcaa gggcgccatg aagctgctgc
     4561 tggcgccgct gctgttgctg cgcgcgctga tcgacgcgct ggccgcgctg cgccgcgtgc
     4621 gtcctgattt cgtgctcggc atgggcggct tcgcttccgg cccgggcggc atcgccgcgt
     4681 ggctgtcgcg caagccgctg ctgatccacg agcagaatgc ggcggccggc atgaccaacc
     4741 gcctgctcgc gcgcatcgcc actcgcgtgc tggaagcgtt cccgcgcacc ttcgccggcg
     4801 cggttaccgt gggcaacccg gtgcgtcgcg gttttgccga actgccggcg ccggaacagc
     4861 ggctgcagca cgacgggcgt gcgcgcctgc tggtcatcgg cggcagccag ggcgcacggg
     4921 cgctgaacca gctggtgccg caggcactcg ctctcgtacc gcgcgagcag cggccgcagc
     4981 tgcgccacca ggccggccgc acgctggagc tggcccagcg cagctacgcc gccaccgatg
     5041 tcgaggtgtc gctggagcag ttcatcaacg acatgccggc cgcatttgcg tgggccgatc
     5101 tggtcatttg ccgcgccggt gcctcgacca tcgcggaaat ctccgcggca ggctgcgcgg
     5161 cgctgctggt gccgttcccg gccgcggtcg acgaccacca gacgcgcaat ggcgagtacc
     5221 tggtgcgcgc cggcgccgcg ttactgctgc cggaaagcac gctcacgccg cagctgctgg
     5281 cgcagacctt gaccgccctg ttggcagatc gcgcaaaact catctcgatg gcgcgtgccg
     5341 cgcgcacctg cgcatggaac gacgctgccg gacgcatcgc gcaactgtgc ctgcagactg
     5401 ccggggccgc cgcatgagcg aatccgcact gcagaacctg atgaacgcgc cgatgcgccg
     5461 cgtgcggcgc gtgcacatgc tcggcatcgg cggcgccggc atggccggca tcgccgaggt
     5521 gctgctcaac ctcggctacg tggtcagcgg caccgatctc aaggacagcg ccgcgaccaa
     5581 gcggctggcg cagttgggcg cgaaggtcta cttcggccac gccaccgcgc atgcgcaggg
     5641 cgccgacgtg gtggtggtgt ccagcgcggt acgggaagac aaccccgaac tgcaatacgc
     5701 gcgcgaacac cgcgtgccgg tggtgcgccg cgcggaaatg ctggccgagc tgatgcgctt
     5761 ccgctacggc atcgcgattg ccggcacgca cggcaagacc accacgacca gcctggtggc
     5821 cagcgtgctg gccgaaggcg ggctcgatcc gactttcgtg gtcggcggca agctcaagtc
     5881 cgccggggcc aatgcgcggc tgggcgccgg cgcctatctc gtcgcggaag cggacgagag
     5941 cgatgcctcg ttcctgcacc tgtcgccgat gatcgcggcg gtcaccaaca tcgacgccga
     6001 tcacctggaa acctacggcg gagatttccg ccgcctgcgc gcgaccttcg tcgagttcct
     6061 gctgcggctg ccgttctacg ggcttgccgt gctgtgcgtg gatgacccgg tggtgcgcga
     6121 gacgctgccc gaggtggggc gcccggtgct gacttatggt ttcgccgggg acgccgatgt
     6181 gcgcgcgctc gaggtgcagg cgcagggtgc cggggcgagt ttcgaggtgc agttccgcga
     6241 cggcgtgcgc gagaagttcc agctgaacct gccgggccgg cacaacgtgc agaacgcgct
     6301 cgtcgcggtg gccatcgcac gtgaactggg cgtgagcttc gatgccatgc gccgtgcctt
     6361 cgccagcttt gaaggggtgg gccgccgctg cgagccgcat ggcgagatcg gtttgccgcg
     6421 cggaaaagta ctggtggtcg atgactatgg gcatcatccg cgcgaaatcg cggcgacctt
     6481 ccaggccatg cgcgcggcct atccgcagcg caggctcttg gtggtgttcc agccgcatcg
     6541 ctacacacgc acgcgagacc tgttcgagga tttctgcgaa gtgctgagca ctgccgatgc
     6601 gctgctgctg ctggaggttt atgcggcagg agaagatgcg atcgaagggg ccgatggacg
     6661 cgcgctggcg cgcggcattc gcgcacgcgg gcgtgtcgag ccggtgttcg tgcgcagtgc
     6721 gcaagaggtg cccgccgcgc tgtcggcgat cgccagcaac ggcgacctgg tactgacact
     6781 cggcgccggc gatgtcggca gcgtgccggc gctgctggag aaattttccg cgagcggtgc
     6841 gccatgaaag gcgtgctgcg cacacacgag cccttgtcac gccataccag ctggcgcgtg
     6901 ggcggacccg ccgatcgtta ctacgaaccg gccgaccgcg aggacctggc gcagttcgtg
     6961 cgcagcctgc cggcgaatga accgatcctg tggctggggc tcggcagcaa cctgctggtg
     7021 cgcgatggcg gtgtgcgcgg cactgtgatc tccctgcacg gcgcgcttga cgatctgcgc
     7081 cgcgaagcgg atgacggcat ttacgccgaa gccggcgtgc actgcgcgcg cctggccaag
     7141 ttcgccgtgg ccgagcattt ttccggagca ggttttcttg ccggcgttcc gggcacggtg
     7201 ggcggcgcgc tggcgatgaa cgcgggcgcg catggcggtg aaacctggaa tgcagtgaat
     7261 gaggcggaag tgatgtatcg cgacggtacg acgcagtggt tggccaggat gcaatttcga
     7321 tattcctacc ggcatgtcga gttgcccgcc ggcatggtcg gtttcgttgc cgcgcgtttc
     7381 cggtttgccc cggatgccgg caagggcgcc gaggcggaaa tgaaatcctg gctggcccgg
     7441 cgcaaggcga cgcagccggt gggcaagccg agcgcaggca gcaccttccg caatcctcct
     7501 ggagatcacg ctgcgcgcct gatcgagcag tgcggcctga agggatatcg catcggcggc
     7561 gccgaggtat cgacgctgca cgccaatttc atcctgaccg gcgacgacgc ccgcgccgct
     7621 gacgtcgaag cgctgatcgc gcatgtgcgc cgcgtggtgg cagaccgcac cggcgttgcc
     7681 ctggaaccgg aagtccgcat cgtgggagag gcgcatgcgt cagcgcacca ctgatccgcg
     7741 cgcattcggc cgcgtcggcg tccttatggg cggctggagt gccgagcggc aggtttccct
     7801 atggagcgga gagggtgtgc tggctgcgct catttccaag ggtgtcgacg tggttggcat
     7861 cgatgccgat cgcgcgaccg tgctgtcgct gggtgcccgc ggcatcgacc gggtgttcaa
     7921 cgtcatgcac ggcaccggcg gtgaagatgg ctcgctgcag gcatgtctcg aactgcagaa
     7981 cattccgtac accggttcgg gtgtgctggc ctcggcgctg gcaatggaca agctgctgac
     8041 caagcgcatc tggaaagccg agggcctgcc gacgccggaa tggctggtgc tgcacgacgc
     8101 acaggaggcg ctgcgcgccg gcgagaagct cggctatccg tatttcgtca aacctgcagc
     8161 tgaaggttcc agcgtgggca tcggcaaggt gaagttcgcc acttccgccg tttcagcctt
     8221 ccggcgtgca cagggcgatt acgacggcat cgatcgcacg gtcgtcgcgg aaaagtttgt
     8281 tgacgggccc gaatacacgt gtgccatcct cgatggcgag gcgctgccga tcatccgcat
     8341 cgagccggac ggcgagttct acgactacaa cgccaagtac atttcggaca atacccgtta
     8401 ccactgcccg gcaggcctgg acgcgccgca ggaagcgcag atcgctgccc tgtgcctgag
     8461 ggcattccaa ctgatcggag ctcatggctg gggccgggtt gatttcatgc tggatccgca
     8521 gcaacagccc tggctgctgg aagtgaatct ggttccgggc atgacctcgc attcgctggt
     8581 gccgatggct gccaaggcgc gcgggctggg ctatgccgac ctgtgctggg ccctgctcga
     8641 gacgacgctg gagggcgggc gatgaacgtg gccgccatgc gcctgcccgg gatggacgtc
     8701 aacaccgcga agcttggacc ggtcctcgca ggagttgccg ccttgctttg cgtgctcatc
     8761 ggcggctggt ggtttggtcg tgtgcatgac gtgcgccagg tgcgcaatct ggagatcgtc
     8821 gggccgttga agcacgtgtc cccggcagac atcgccacgg tcctgcagcc gcatgtggac
     8881 agtggattcc tggcgctcga cctggacgaa gcccgggaca gcctcgagca gttgccgtgg
     8941 gtgagccgcg cgcgtgccga gcgctactgg ccggccggtg tccgcgtgca ggtctgggag
     9001 cgcgaagccg gcgcgcgctg gaatgagcgc cagctgctgg acgtggacgc ccatgcctac
     9061 accccggcgc catcagacct ggttgggccg ctgccgacgc tgcgcggacc ggtcggccat
     9121 gaaaaggaag tgctcgacgc tttccagcgc tacagcaagc gggtggaggg cacgccattc
     9181 gagttggccg gaatcgccct ggacgcgcgt ggtgaatggt cggcgacaac gaagggcggt
     9241 atcggattgc ggctggggcg cggttcgccg gacgcccggt tcgatgtgct ggtgacgacg
     9301 gtgagcaagt ctttggccga tcgtctgggg caggttgatt acgtcgatct gcgttacacc
     9361 aacggatttg cggtgggctg gaaggcgggc gctcaacagc ctgctccggg ggctgcgcat
     9421 ggctaagaaa gacgcaagga acctgctggt tggcatcgat attggcacct ccaaggtggt
     9481 ggcggtgatc ggtgaggtca atcacgacgg caacatcgaa gtggtcggcg agggttcggc
     9541 gccggcacgc ggcatgaatc gcggcgacgt ggtgaacatc gaagccaccg ttcaatccat
     9601 acgccgcgcc gtggagaacg ccgagcacat ggcgggttgc cgcgcgcagt cggtctacgt
     9661 cggcatttcc ggcacgcata tcaagagtta caactcgacc ggcgttgcgc cggtgcgcgc
     9721 gcgcgaggtg agcgacaagg acgtcgagag cgtgattgac gcggctcgcg ccatagcgat
     9781 tcccgcggac cagaaaatcc tgcacgtggt gccgcaggaa ttcgtggtcg acggacgtga
     9841 aggcatccac gagccggtcg gcatgttcgg cgtgcgcctc gaagccaagg tccatatggt
     9901 gaccggctcg ctgtcggccg cgcagaacat ctacaagtgc gtcgaatcct gcggcctttc
     9961 ggtcgacaag ctcatcctgc agcacctggc ctcttcctat gccgtgctgc ttcccgatga
    10021 acgcgacctg ggcatctgca ggg
//
LOCUS       DASZZC010000100         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_265580,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000100 DASZZC010000000
VERSION     DASZZC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265580"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHE37_02400"
     CDS             <1..657
                     /locus_tag="VHE37_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT34407.1"
                     /translation="MLISFTVMALKALQVRRVESGNKAFLALFHSKSGDFGALHRVMT
                     GAEAAGDVEWIDDAPLSRLFMAGAEELHQRLEGERNRRGPALSSQSIEAIRAAIDATL
                     VEESQALSRSMVLLTIAISGGPFIGLLGTVVGVMITFAAVAAAGDVNVNAIAPGIAAA
                     LAATVAGLMVAIPALFGYNYLITRIKEIGAQMHVFVDRLITRMAENYNQPAALHEMAD
                     "
     gene            670..1074
                     /locus_tag="VHE37_02405"
     CDS             670..1074
                     /locus_tag="VHE37_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT34408.1"
                     /translation="MQAQSEEKAYDDINITPMLDLAYVLLVIFIIMTTAAVQGLKVNL
                     PKASDQPSLSKPKTVAITITNDGKIYMDAIPVSMNELEDRLRTKKAQIPDLPVIVRGD
                     GATQYQNVIDVLALLGRLDITQLGLVTQRLVK"
     gene            1081..>1500
                     /locus_tag="VHE37_02410"
     CDS             1081..>1500
                     /locus_tag="VHE37_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34409.1"
                     /translation="MRERKPRITPKGQLMIGAAIVVVAAVLAVSSYFLIKSSSKSARK
                     QVPEVAHVALLTPPPPPPPPPPPPPPEQKIEPIKQEFQKQEQQEPDAPKGPALDAKGE
                     GPGDAFGLEGKQGGSDFVGGSGNGNSRYGWYATMVQQR"
BASE COUNT          275 a          493 c          514 g          218 t
ORIGIN      
        1 atgctgatca gcttcaccgt gatggcgctg aaggcgttgc aggtgcggcg cgtggaaagc
       61 ggcaacaagg ccttcctcgc gctgttccac tccaagtccg gcgatttcgg cgcgctgcac
      121 cgggtgatga ccggcgcgga agcggccggt gacgtggagt ggatcgacga cgcgccgctg
      181 tcgcgcctgt tcatggccgg cgccgaggaa ctgcaccagc ggctggaagg cgagcgcaac
      241 cggcgcggcc cggcgctgtc gtcacagtcg atcgaggcga tccgcgcggc catcgacgcc
      301 acgctggtgg aggaatcgca ggcgctgagc cgctcgatgg tgctgctgac catcgccatc
      361 tccggcggtc cgttcatcgg cctgctcggc accgtggtgg gcgtgatgat caccttcgcc
      421 gcggtggccg cggcgggcga cgtcaacgtc aacgccatcg cgccgggcat cgccgcggcg
      481 ttggcagcga cggtggcggg tctcatggtc gccatcccgg cgctgttcgg ctacaactac
      541 ctgatcacgc gcatcaagga aatcggcgcg cagatgcatg tgttcgtcga ccggctgatc
      601 acgcgcatgg cggagaacta caaccagccc gccgcgctgc acgagatggc ggactgaggg
      661 cgcgccgcca tgcaagcgca atccgaagag aaggcatacg acgacatcaa catcacgccg
      721 atgctggacc tggcgtacgt gttgctggtg atcttcatca tcatgaccac ggccgcagtg
      781 caggggctca aggtcaacct gcccaaggcc agcgaccagc cgagcctgtc caagccgaag
      841 accgtcgcca tcaccatcac caacgacggc aagatctaca tggacgcgat cccggtctcg
      901 atgaacgagc tggaggatcg cctgcgcacg aagaaggcgc agataccgga cctgccggtg
      961 atcgtgcgcg gcgacggcgc cacccagtac cagaacgtga tcgacgtgct ggcgctgctc
     1021 ggccgcctgg atatcacgca gctgggcctg gtcacgcagc gcctggtgaa gtagtgcgtc
     1081 atgcgcgagc gcaagccacg aatcacgccg aagggccagc tgatgatcgg cgcggccatc
     1141 gtggtcgtgg ccgccgtgct cgcggtcagc tcgtacttcc tgatcaagag ctcgtcgaag
     1201 agcgcgcgca agcaggtgcc ggaagtggcg cacgtggctc tgctgacgcc gcctccgccg
     1261 ccacctccgc cgccgccacc tccgcctccc gagcagaaga tagagccgat caagcaggag
     1321 ttccagaagc aggagcagca ggagcccgat gcgcccaagg ggccggcgct cgatgccaag
     1381 ggcgagggcc cgggcgacgc ctttggcctc gagggcaagc agggcggcag cgatttcgtc
     1441 ggcggcagcg gcaatggcaa cagccgctac ggctggtacg cgaccatggt gcagcagcgc
//
LOCUS       DASZZC010000101         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_8741,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000101 DASZZC010000000
VERSION     DASZZC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8741"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VHE37_02415"
     CDS             <1..1113
                     /locus_tag="VHE37_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT34410.1"
                     /translation="QPYLHYQIWAPRGSLLLRSHQAPLQPMSPTAAGGLSDQQVNGDR
                     WRVYVVASARSGLSIQIGEPYMHRREAGQGVILVMLITMGVCSAVLLLALSTMIDRIA
                     AQMGRSVREIGERSAADLTPLPAPLLPTEFGPLLLALNGLLTRLEKALAQERRFAADA
                     SHELRTPLAVIKTQAQIARRARHPAETEQALQKLEAGVDRAVHLVEQLLAAARIEPMA
                     AQGEPAVPIDVRAEVQQLLDTYRNLIERRGMRVQCDIADSRWRGNPALARVLLRNLFD
                     NALRYGGGEVRLRYSADADGQVLQVLDDGPGIAPDQRERVFDPFYRVPGAAGNGSGLG
                     LAIVRQIVSAIGGRIELGAGIGGDGLGVSVYLPAAD"
     gene            complement(1114..>1755)
                     /locus_tag="VHE37_02420"
     CDS             complement(1114..>1755)
                     /locus_tag="VHE37_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT34411.1"
                     /translation="AMLLAAGWLALAAVLLGAFAAADNSAGIGRLAGKAAGLVLACIG
                     VAQVAGALSGAPTPWQPLAAFAARESSTAPAQQQTRNSFQKLDSLAGLEQALAQAQGR
                     PVMLDFYADWCLACKELEHQTFADPRVRAQLSRMVLLQADVTRNTDAQQAMLKRYQLF
                     GPPGIVFFDPRGHELSDRRVIGYQDADRFLSSLGPAGECSVQLVAATPSSASC"
BASE COUNT          265 a          603 c          636 g          251 t
ORIGIN      
        1 cagccctacc tgcactacca gatctgggca ccgcgcggca gcctgctgct gcgctcgcac
       61 caggccccgc tgcagcccat gtcgccaacg gcggcgggcg gcctgagcga ccagcaggtc
      121 aatggcgacc gctggcgtgt ctacgtggtg gcttccgcgc gcagcggctt gtccattcag
      181 atcggcgaac cgtatatgca ccggcgcgaa gccggccagg gcgtgatcct agtcatgctc
      241 atcaccatgg gcgtgtgcag cgcggtgctg ctgctggcgc tgtcgaccat gatcgatcgc
      301 atcgccgcgc agatgggccg ctcggtgcgc gagatcggcg agcgcagtgc ggccgatctc
      361 acgccgctgc cggcccccct gctgccgacc gagttcggtc cgctgctgct ggcgctcaac
      421 ggactgctca cgcggctgga gaaggcgctg gcgcaggagc gccgtttcgc cgccgacgcc
      481 tcgcatgagt tgcgcacgcc actggcggtg atcaagaccc aggcgcagat cgcgcggcgc
      541 gcccgccacc ctgccgaaac cgagcaagcg ctgcagaagc tggaagcggg cgtggaccgc
      601 gccgtgcacc tggtcgagca gctgcttgct gccgcgcgca tcgagccgat ggcggcgcag
      661 ggcgagccgg cagtgccgat cgatgtgcgc gccgaggtgc agcaattgct cgacacctac
      721 cgcaacctga tcgagcgccg cggcatgagg gtgcaatgcg acatcgccga cagccgctgg
      781 cgcggcaacc cggcgctggc gcgcgtcctg ctgcgcaacc tgttcgacaa cgcgctgcgt
      841 tacggcggtg gcgaggtgcg cctgcgctat tccgccgatg ccgatggaca ggtgctgcag
      901 gtgctggacg acgggcccgg catcgcgccg gaccagcgcg agcgcgtgtt cgatccgttc
      961 taccgtgtgc cgggagccgc cggcaacggc agcgggctag gcctggccat cgtgcgccag
     1021 atcgtgtcgg ccatcggagg gcgcatcgaa ctcggcgcgg gaatcggcgg ggacggcctc
     1081 ggcgtcagcg tgtacttgcc agccgcggac tgatcagcac gatgcgctgg aaggggtggc
     1141 cgcaaccagc tgcacgctgc actcgccggc ggggcccagg ctggagagga agcggtcggc
     1201 atcctggtaa ccgatgacgc ggcggtcact cagttcgtgt ccgcgcggat cgaagaagac
     1261 gatgccgggc gggccgaaca gctggtagcg cttgagcatg gcctgctgtg cgtcggtatt
     1321 gcgggtgacg tcggcctgca gcagcaccat gcgcgacagc tgcgcgcgca cgcgcggatc
     1381 ggcaaaggtc tggtgctcca gttccttgca cgccaggcac cagtcggcat agaagtcgag
     1441 catcaccggc cggccctgcg cctgcgccag tgcctgctca agaccggcga ggctgtccag
     1501 tttctggaag ctgttgcgcg tctgctgctg cgcgggcgca gtcgaggatt cacgcgcggc
     1561 gaatgcggcc agcggctgcc acggcgtcgg tgcaccggac agcgcgccgg ccacctgggc
     1621 cacgccgatg caggccagca ccagtccggc cgccttgccg gccaggcggc cgatgccggc
     1681 actgttgtcg gcggccgcga acgcaccgag cagcacggcg gccagggcca gccagccggc
     1741 tgccagcagc atggc
//
LOCUS       DASZZC010000102         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1035865,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000102 DASZZC010000000
VERSION     DASZZC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035865"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VHE37_02425"
     CDS             complement(<1..1248)
                     /locus_tag="VHE37_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34412.1"
                     /translation="MQRLLLAVLASVACVAARAGDGGITDLSIEQLSNIQVTSVSKSG
                     EALQNAPATIYVITHDDIVRSGVTSIAEALRLAPNLQITQYSSTNYVAGARGFAGAQE
                     VQNFSNKLLILIDGRSVYTPIYSGVYLDAQDVALADIDRIEVISGPGATLWGANAMNG
                     VINIITRAAYLTQGTMVDAGYGDAERTLTARYGAKAGDAWSWRVYGKTFEHDPLELRD
                     GTSARDGWDKAQGGFRADWTSIADTVTTQGDGYRAFDQQAGTAKARVEGANLLARWQH
                     RGTTEWQLQSYYDYTERAQPPGGVAFALRTYDVEWQQKVNATRQRIVWGAGGRMHQYY
                     ITPTQPLSFDPAERTLGLGDLFVSDTISLPAEVALTLGLKAERDPYSGWALLPDVRAG
                     WHVAERQLVWASASRAIRSPTPFD"
     gene            complement(1439..2038)
                     /locus_tag="VHE37_02430"
     CDS             complement(1439..2038)
                     /locus_tag="VHE37_02430"
                     /inference="COORDINATES: protein motif:HMM:NF042726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6655 family protein"
                     /protein_id="HVT34413.1"
                     /translation="MLLRALLLAPLLCAAGCTTFRVTEPGQTATEQLLVSTAVDHAVA
                     RLDPQLAKGTKVFLDTQFYDSASSDQLPKYTIGALRQRLLDLGLLLVDDRKNAQAILE
                     LRTGTQSINHKTMLIGLPSIPIPIPFAGTVSTPEIALFSLDRQTGVARLALTPYWSAN
                     GAPAGNAATADDYGMSKRIKYSVLFVSWSDDDLGPADGR"
     gene            2254..>2647
                     /locus_tag="VHE37_02435"
     CDS             2254..>2647
                     /locus_tag="VHE37_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34414.1"
                     /translation="MRSDPVFVRPFLLMLPVLLASCGGGGGGGDSAGLGSGGGSSGGT
                     GICNALNGYLTTDSSAAGMVTGYSFAVFSKSNILLTCAGGDQQIGAIRAIASASKLPS
                     AAAIMTLVDSGSLNLDLPVSVYLLGSGVN"
BASE COUNT          426 a          861 c          899 g          461 t
ORIGIN      
        1 gtcgaacggc gtgggcgagc ggatggcgcg cgaggcgctg gcccagacca gttgccgctc
       61 cgccacgtgc cagcccgcgc gcacgtcggg cagcagcgcc cagccggaat aaggatcgcg
      121 ctcggccttg agtcccagcg tcagcgccac ctcggcgggc agtgaaatgg tgtcgctgac
      181 aaaaagatcg cccaggccca gtgtgcgctc ggcgggatcg aaggagagcg gctgggtcgg
      241 cgtgatgtag tactggtgca tgcgcccgcc tgcgccccag acgatgcgct ggcgcgtcgc
      301 gttgaccttc tgctgccatt ccacgtcgta ggtgcgcaac gcaaaggcaa cgccgccggg
      361 tggctgtgca cgctcggtgt agtcatagta agactgcagc tgccattcgg tggtgccgcg
      421 gtgctgccag cgcgccagca ggttggcacc ttccactcgc gccttcgccg tgcccgcctg
      481 ctggtcgaat gcgcggtagc catcgccctg cgtggtgacc gtgtcggcaa tgctggtcca
      541 gtcggcgcgg aagccgccct gtgccttgtc ccagccatcc cgcgcgctgg tgccatcgcg
      601 cagttccagc ggatcgtgtt caaaggtctt gccgtacacg cgccaggacc aggcatcgcc
      661 agccttggcg ccgtagcgcg cggtgagcgt gcgctcggca tcgccgtagc ccgcgtcgac
      721 catggttccc tgcgtgagat aggccgcgcg cgtgatgatg ttgatgacgc cgttcatggc
      781 attggcgccc cacagcgtgg cgccgggtcc gctgatcacc tcgatgcggt cgatgtcggc
      841 cagcgccacg tcctgcgcgt cgagatacac gcccgagtag atcggcgtgt atacgctgcg
      901 tccgtcgatc aggatcagca gcttgttgga gaaattctgc acttcctgcg cgccggcgaa
      961 gccgcgcgcg ccggccacgt agttggtgct gctgtactgg gtgatctgca ggttcggagc
     1021 caggcgcagc gcctcggcaa tgctggtgac gcctgagcgc acgatgtcgt cgtgggtgat
     1081 gacgtagatg gtcgccggcg cgttctgcag ggcctcgccc gacttggaga ccgaggtaac
     1141 ctggatgttc gacagctgct cgatgctgag gtcggtgatg ccgccatcgc ccgcgcgtgc
     1201 tgcaacgcag gcaaccgacg ccagcacggc cagcaacagc ctctgcatgt tcagcaaaac
     1261 ggacgacggt tcccctgctc ccacggtcaa tctcccgcat ggacgctgcc gcagacggcg
     1321 gcccaaagcg cagattgagg atcgccggcc gccgcgtcaa acgcgaattt gcacaggctg
     1381 catgtgcata agcaccgctt atggggaata cgctaccgcg cgtcaggaca aggcggcatc
     1441 agcgtccgtc ggccggcccc aggtcgtcgt cgctccagga cacgaacagg accgaatact
     1501 tgatccgctt ggacatgccg tagtcgtcgg ccgtagctgc gttgccggca ggcgcgccgt
     1561 tggcgctcca gtacggcgtc aacgccagcc gcgccacgcc ggtctgccgg tcgagcgaga
     1621 acagcgcgat ttccggcgtg ctgaccgtgc cggcaaacgg aatcgggatg gggatcgagg
     1681 gcaggccgat cagcatggtc ttgtggttga tcgactgcgt gccggtgcgc agttcgagta
     1741 tcgcctgcgc gttcttgcgg tcgtccacca gcagcagccc caggtcgagc aggcgctggc
     1801 gcaatgcgcc gatggtgtac ttgggcagct ggtccgacga tgcgctgtca tagaactgcg
     1861 tatcgagaaa caccttggtg cccttggcca gttgcggatc gagccgggcc acggcgtgat
     1921 cgaccgcggt gctgaccagc agctgctcgg tggcggtctg ccctggctcg gtgacgcgga
     1981 aggtggtgca gccggcagcg cacagcaacg gtgcaagcag cagcgctcga agcagcatcg
     2041 gaatgccccg catggatcgg tgacactact gacacccgcg cactgcaccg gttcgggcgg
     2101 cgcgtgggcg cagtcttaca agtgatcgcc ttccggcggc gcgtgaggca tattcactcc
     2161 ggtagcggtc cgatctgcaa agctttgtag aggatgacct ggagactacg tcgcggccgc
     2221 acaatcccgc cgcggcagac aaggagtcga atcatgcgca gtgaccccgt gttcgtacgt
     2281 ccgttcctgc tgatgctgcc ggtgctgctc gcgtcctgtg gaggcggcgg tggtggtggc
     2341 gacagcgccg gcctgggcag cggcggcggc agttccggcg gcaccggcat ctgcaatgcg
     2401 ctcaacggtt acctgaccac cgattcctcc gctgccggca tggtcaccgg ctacagcttc
     2461 gcggtattca gcaagtcgaa catcctgctc acctgcgccg gcggcgacca gcagatcggc
     2521 gccatccgcg ccatcgcctc ggccagcaag ctgccgtcgg cggcggcaat catgacgctg
     2581 gtcgacagcg gcagcctcaa cctggacctg ccggtctcgg tttatctgct cggcagcggc
     2641 gtcaact
//
LOCUS       DASZZC010000103        10249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1162669,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000103 DASZZC010000000
VERSION     DASZZC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162669"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VHE37_02440"
     CDS             <1..760
                     /locus_tag="VHE37_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVT34415.1"
                     /translation="EGPVTRIGIMRQERRNAINRAAAEQLADAFRHFDEDDSARVAIL
                     HGEGEHFCAGADLTAVASGDAAQMNRLDMTGDGPMGPTRMQLSKPVIAAVSGYCVAGG
                     LELALWCDLRVADETAVFGVFCRRFGVPLIDGGTVRLPRLIGMSRALDMILTGRAVDA
                     REAASWALVNRLVPKGHALAEAQKLATELAALPQTCMRNDRHSAYTQWGLSESEAIAE
                     EFRLGLASLASGEAASGSARFTSGDGRHGGSLKP"
     gene            complement(811..3855)
                     /locus_tag="VHE37_02445"
     CDS             complement(811..3855)
                     /locus_tag="VHE37_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34416.1"
                     /translation="MKITRAGWLSARIGALSLLAAASGAVCAQDNNDAAAAPIPVTPL
                     RNDTAPVKTKEAATELDAVEVTGSRIKAVDMETSNPVFTIGRDAIRSTGATTIGELLQ
                     SIPAATGVVTNPQVNNGGGNGGASVNLRGLGGERTLVLLDGKRIIGTTFSDISYVDLN
                     SLPINLIDHIEVLKEGASAIYGSDAIGGVVNIISRKNFTEGEGEITYGLTQHGDGAHK
                     NINVTYGSGSSESHVVFGLEYEMQNSIAAKDRDLTSTPTAYYFGQLDPNAGTSGRAPT
                     GRWFLPPQVFTGGDGVQCGQAPGTNPDGTPNPGVSPSVTRIAGHDGSTSTDYRCFNNN
                     SGPGATDRYNFLPDNLLLTPAKRYEMFAMGEHQFGDDMQFYGAGFFTHVVSNSQLAAE
                     PFDNGTVQAVIGPTTISGQNQYNPFRQDISTFALRPTLVGDRIETVDTSTYQGTVGLK
                     GFLFDDAFQWDGSYTYGRIDQGESDYGFLNFSNLKNQVGPSVGGVCGTTANPIPQCTP
                     INVFGTIAPTIAELGTTANTQTEQDQQQLLASISGDLLKLPAGALGSAFGIEYRKYNF
                     NRVPDGLEQAFQLSENNSKPTQGSYDVKELFAELRIPVLADKPFVESLTLTPGVRYSK
                     YSSFGSTTNAKYGIEYRPYGDLLIRATYADIFRAPTVNDLFKGNLQDAPGYSDPCNAY
                     GSAGNPRTANGDLACQNVPTNGSFQQANTQASAVRTGNPNLRPETGFTTDFGLVYSPS
                     FYRPLTIEMDYFKYAINDTIGLLDTQSAVNACFNTGEFCQYVGRSSDGQLALGTEPTI
                     NNGSLVTQGLDFGIKLSYPNTRFGSFLISADTTYLQIYDTATVVAGQVLNYESLAGQT
                     DGGSTGTFPRIKSFMTVAWNMGSWSASLRDRLIGNVGEGSIYFNGSPNAIIDLADGVF
                     GACGSTTADSTQVDVGGGNMQTIACRRNVGMANYLDLSGTYALDRFATTITFGITDIT
                     NTGGKFVASAAAGGLGTGYPTDPATYDMTGRAFFATIKTNFK"
     gene            complement(4181..4579)
                     /gene="rplQ"
                     /locus_tag="VHE37_02450"
     CDS             complement(4181..4579)
                     /gene="rplQ"
                     /locus_tag="VHE37_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HVT34417.1"
                     /translation="MRHANSGRAFGRSSSHRKATMQAVVNALIKHEMIKTTVAKAKEL
                     RRAAEPLITRAKKDTLANRRIAFNRLRDRDAVTKLFTELGPRYTTRPGGYTRILRCGF
                     RAGDKAPMAYVELVDRPRPAAQADAAAASA"
     gene            complement(4605..5588)
                     /gene="rpoA"
                     /locus_tag="VHE37_02455"
     CDS             complement(4605..5588)
                     /gene="rpoA"
                     /locus_tag="VHE37_02455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HVT34418.1"
                     /translation="MQNVIADLLKPRTIEVEAVSENRARIALEPMERGFGHTLGNALR
                     RILLSSIPGAAITEVQIEGVVHEYSAIEGVQEDVVDILLNIKNIAVRMHSREEATLRL
                     EKSGKGPVKASDIKLDHDVEIVNPDLVLAHLTGGKLNMTLKVTRGRGYVPALSTARPG
                     EEDGHSIGALKLDASYSPVRRVSYAVERARVAQRTDLDKLILDVDTNGGIDASEAVRL
                     AARILRDQLGVFVDLEAEAAAAQAPGKKDLSPILFRPIDDLELTVRSANCLKAENVYY
                     IGDLVQRTETELMKTPNLGKKSLNEIKEALKGHDLELGMKLENWSSPKTPA"
     gene            complement(5679..6299)
                     /gene="rpsD"
                     /locus_tag="VHE37_02460"
     CDS             complement(5679..6299)
                     /gene="rpsD"
                     /locus_tag="VHE37_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HVT34419.1"
                     /translation="MARYIGPKCKLSRRAGADLLLKSRARSLETKCKLDTPPGQHGAG
                     KQRLSDYAGQLREKQKLKQMYGVLERQFRNYYLSASSGKGATGLNLLLMLEARLDNVI
                     YRMGFGRTRSECRQLVGHKAIMVNGKSVNIPSYQVQVGDVIEVREKSRNQSRIAESLS
                     IATQAGLAEWVEVDEKGLKGTFKALPTRDQILPDINENLVVELYSK"
     gene            complement(6315..6728)
                     /gene="rpsK"
                     /locus_tag="VHE37_02465"
     CDS             complement(6315..6728)
                     /gene="rpsK"
                     /locus_tag="VHE37_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HVT34420.1"
                     /translation="MANPAANAPAGAAAPKRKKVKKNVTDAVAHVHASFNNTIILITD
                     RQGNTLSWSTAGAAGFKGSRKSTPFAAQVAADKAATAAAEHGVKNLEVRVKGPGPGRE
                     SAVRALNAAGMKITNITDVTPIPHNGCRAPKRRRV"
     gene            complement(6752..7126)
                     /gene="rpsM"
                     /locus_tag="VHE37_02470"
     CDS             complement(6752..7126)
                     /gene="rpsM"
                     /locus_tag="VHE37_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVT34421.1"
                     /translation="MARIAGVNIPDKKHTVIALRAIYGIGATRAKKICAAAGIKPDVQ
                     IRTLSEGELDKLRAEVARFTVEGDLRREVSMSIKRLMDLGCYRGQRHRRGLPVRGQRT
                     RTNARTRKGPRRGTVATNKKTV"
     gene            complement(7208..7321)
                     /gene="rpmJ"
                     /locus_tag="VHE37_02475"
     CDS             complement(7208..7321)
                     /gene="rpmJ"
                     /locus_tag="VHE37_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVT34422.1"
                     /translation="MKVRASVKKICRNCKVIRRNGVVRVICSDPRHKQRQG"
     gene            complement(7353..8705)
                     /gene="secY"
                     /locus_tag="VHE37_02480"
     CDS             complement(7353..8705)
                     /gene="secY"
                     /locus_tag="VHE37_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVT34423.1"
                     /translation="MAKTPATAAGSMVPDLAKLGDVRARLLFLLGAIIVYRIGSFIPV
                     PGINPDQLAALFNKQTGGSILDMFNMFSGGSFKRLSVFALGVMPYISASIIVQLLTAV
                     IPQLKELRKEGDSGRRTLTKYTRYGALGLALFQSIGMAYGFQAKSQVVLNPGFGFMFT
                     ATISLVTGTMFLMWLGEQITERGVGNGMSMLIFSGIVADMPRALGATAQLVGSGEINA
                     FQVILVFAIAIAVTWGVVFVERAQRRIPIHHARRQQGRKLFAAQTQHLPLKLNMSGVI
                     PPIFASSIILFPATIIGFLGQEGSGGFLQQLSNALSPGQALYTIVYAALIIFFCFFYT
                     ALVFDSRETAENLKKSGAFIPGVRPGAMTARYIDQVLVRLTVIGAAYVTVVCLTPEFL
                     TTTWHVPFYFGGTSLLITVVVVMDFMAQLQAHLMSHQYEGLMKKANLRSGGAVGSKTS
                     "
     gene            complement(8718..9149)
                     /gene="rplO"
                     /locus_tag="VHE37_02485"
     CDS             complement(8718..9149)
                     /gene="rplO"
                     /locus_tag="VHE37_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HVT34424.1"
                     /translation="MKLNSIKPAKGAKKDRRRVARGGGSGLGKTAGRGHKGQHARSGG
                     FNKLGFEGGQMPIQRRLPKRGFNSPSAGLTAEVRLSDLSKMKATDIDLATLKAEGVVS
                     PRAQTAKLIKSGELTRKVVLKGILASKGAREAVAAAGGSIA"
     gene            complement(9151..9351)
                     /gene="rpmD"
                     /locus_tag="VHE37_02490"
     CDS             complement(9151..9351)
                     /gene="rpmD"
                     /locus_tag="VHE37_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HVT34425.1"
                     /translation="MTAKKDSKGQIKVTLMRSLAKRLPNHQDCVRGLGLSRRHQTVTL
                     QPTPEIMGLVNKVSYMLKVEQA"
     gene            complement(9366..9884)
                     /gene="rpsE"
                     /locus_tag="VHE37_02495"
     CDS             complement(9366..9884)
                     /gene="rpsE"
                     /locus_tag="VHE37_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HVT34426.1"
                     /translation="MPKPNIPVEDIQAGGDLKEKLVAVNRVAKVVKGGRQFGFTALTV
                     VGDGSGRVGFGYGKAREVPVAISKAMEKARKNMITVPLKGLTLQHEIWGTHGSTRVFM
                     RPASDGTGVIAGGAMRAVFEVAGVQNVLAKAFGSRNPINMVRATIDALKKMNIPSEIA
                     AKRGKSVEEILA"
     gene            complement(9888..10238)
                     /gene="rplR"
                     /locus_tag="VHE37_02500"
     CDS             complement(9888..10238)
                     /gene="rplR"
                     /locus_tag="VHE37_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HVT34427.1"
                     /translation="MKDKNDSRLRRARRTRAHIRELGMHRLSVHRTPRHIYAQVIAPG
                     ASTTVAAASTVEEIDGLNGTGNVDAAKKIGALIAERAKAAGVTKVAFDRSGFKFHGRI
                     KALADAAREAGLEF"
BASE COUNT         1800 a         3139 c         3273 g         2037 t
ORIGIN      
        1 cgaaggtccc gtgactcgca tcggcatcat gcggcaggag cggcgcaacg cgatcaaccg
       61 cgctgcggcc gaacagctgg ccgatgcctt ccgccacttt gacgaggacg atagcgctcg
      121 cgtcgcgata ttgcacggag agggcgaaca cttttgcgcc ggcgcagatc tcactgcagt
      181 tgccagcgga gacgctgcgc agatgaatcg cctggacatg accggcgatg gccccatggg
      241 ccccacccgc atgcagttga gcaagccggt gatcgcggct gtctctggct actgcgttgc
      301 aggcggactc gagctcgcgc tgtggtgcga tttgcgcgtg gcggacgaga ccgccgtctt
      361 cggagtcttc tgccgccgct tcggtgtgcc tctgatcgac gggggcaccg tgcgcttgcc
      421 gagattgatc ggaatgtcgc gcgcgctcga catgattctt accggacgcg cggtagacgc
      481 ccgcgaagct gcatcatggg ccttggtcaa ccggctcgtc cccaaaggcc atgcacttgc
      541 agaagcacag aagctggcga cagaacttgc tgcccttcca caaacctgca tgcgcaatga
      601 tcgccactct gcctacaccc aatgggggct gtcggagagc gaagccatcg ctgaggaatt
      661 tcgattggga ctcgcctcgc ttgcgtcggg cgaagccgca tccggctcgg cccgcttcac
      721 cagtggcgat ggacgccatg gaggatccct caagccctga aacggacaag gggccttgcg
      781 gccccttgtc gtttacggca cgcaaccggg ctatttgaaa ttggtcttga tggtagcgaa
      841 gaacgcgcgc cccgtcatgt cgtaggtggc cgggtcggtc gggtacccgg tacccaggcc
      901 tcccgccgct gccgacgcca cgaacttgcc gccggtgttg gtgatgtcgg tgatgccgaa
      961 agtaatggtg gtggcgaatc gatcgagtgc gtaggttccg ctcagatcga gatagttggc
     1021 cattcccaca ttcctgcggc aagcgatagt ctgcatgttg ccgccgccga catccacctg
     1081 ggtgctgtcg gcagtggtgc tgccgcaagc accgaacacg ccgtcggcca ggtcgatgat
     1141 cgcgttgggc gagccgttga agtagatgct gccctcgccg acattgccga tcagtcggtc
     1201 gcgcagcgat gcgctccatg agcccatgtt ccacgccacc gtcatgaacg acttgatgcg
     1261 cgggaaagtg ccggtgctgc cgccgtcggt ctggccggcc agcgattcgt agttgagcac
     1321 ctgcccggca acgacggttg ccgtgtcata gatctgcagg tacgtggtat ccgcgctgat
     1381 caggaagctg ccgaagcgag tattcgggta gctcagcttg atgccgaaat cgagcccctg
     1441 cgtgaccagc gagccgttgt tgattgtggg ttcggtgccc agcgccagct ggccatcgct
     1501 ggagcgacct acgtactggc agaactcacc ggtgttgaag cacgcattca cggcgctttg
     1561 cgtgtccagc agcccgatgg tgtcgttgat ggcgtacttg aagtaatcca tctcgatggt
     1621 gagcggacga tagaacgacg ggctgtagac caggccgaag tcggtggtga agccggtttc
     1681 cggacgcaag ttcgggttgc cggtacgcac cgcgctggcc tgcgtattgg cctgctggaa
     1741 gctgccgttg gtaggaacgt tctggcaggc caggtcgccg ttggccgtgc gcggattgcc
     1801 agccgagcca taagcgttgc agggatcgct gtatcccggc gcgtcctgca ggttgccctt
     1861 gaacaggtcg ttgacggtgg gagcgcggaa gatgtcggca taggtggcgc ggatcagcag
     1921 atcgccatac ggccggtatt cgatgccgta cttcgcgttg gtggtgctgc cgaagctcga
     1981 gtactttgaa tagcgcacgc ccggcgtcag cgtgagtgat tccacgaaag gcttgtcggc
     2041 cagcaccggt atgcgcagct cggcgaacaa ctccttcacg tcatacgagc cttgcgtggg
     2101 cttcgagttg ttttccgaca actggaacgc ctgctcgaga ccgtccggaa cgcgattgaa
     2161 gttgtacttg cgatactcga tgccgaaggc ggacccgagc gcacccgccg gcagcttcag
     2221 caggtctcca ctgatgctgg ccagcagctg ctgctggtct tgctccgtct gcgtgtttgc
     2281 ggtggtgcca agttcggcga tggttggcgc aatggtgccg aatacgttga tcggcgtgca
     2341 ttgtggaatt ggattggcgg tagtgccgca aacgccgccc accgacgggc caacttgatt
     2401 cttcaggttg gagaagttaa gaaagccgta atcgctttca ccttgatcga ttcgcccgta
     2461 cgtataggat ccgtcccatt ggaaagcatc atcgaaaagg aagcctttca ggcctaccgt
     2521 tccttgatac gtgctggtat ccactgtctc gatgcgatcg cccaccaagg tagggcgcag
     2581 ggcgaaagtg ctaatgtcct ggcggaaagg gttgtattga ttttggcctg agatcgtcgt
     2641 cggtccgatt accgcctgca ccgtgccatt gtcgaacggc tccgcggcca gttgcgagtt
     2701 ggacaccacg tgagtgaaga agccggcgcc atagaactgc atgtcgtcgc cgaactggtg
     2761 ctcgcccatg gcgaacatct cgtagcgctt ggccggcgtc agcagcaggt tgtcgggcag
     2821 gaagttgtaa cggtcggtgg cacccgggcc cgaattgttg ttgaaacagc gatagtcggt
     2881 agaagtgctt ccatcgtgac ccgcaatgcg ggtaacgctc ggcgaaacgc ccggattggg
     2941 cgtcccatcg ggattcgtgc ctggtgcctg gccgcactgc acgccgtcgc cgccggtaaa
     3001 aacctgagga ggcaggaacc aacgaccagt tggcgcgcgt cccgaagtcc cggcattggg
     3061 gtcgagctgg ccgaagtagt aggccgtcgg cgtcgaggtc agatcgcggt ccttggccgc
     3121 gatggagttc tgcatttcgt actcgagccc gaataccacg tggctttccg acgaaccgct
     3181 gccataggtc acgttgatgt tcttgtgcgc accgtcgccg tgctgggtca gaccgtaggt
     3241 gatctcacct tcgccctcgg tgaaattctt gcgcgagatg atgttgacca cgccgccgat
     3301 ggcatccgaa ccgtagatgg ccgacgcgcc ttccttgagc acctcgatgt gatcgatcag
     3361 gttgatcggc agcgagttga ggtccacata gctgatgtcg gaaaacgtgg tgccgatgat
     3421 gcgcttgccg tcgagcagca ccagcgtgcg ctcgccgccg aggccgcgca gattcaccga
     3481 ggcgccgccg ttgccgccgc cgttgttcac ctgcggattg gtcaccacgc cggttgccgc
     3541 cgggatgctc tgcagcagtt cgccgatggt ggtggcgccg gtcgagcgga tggcatcgcg
     3601 gccgatggtg aacaccggat tggaagtctc catgtccact gccttgatgc gcgagccggt
     3661 gacttccacc gcgtccagtt ccgtcgctgc ttccttggtt ttgaccggcg cggtgtcgtt
     3721 gcgcagcggc gtgacaggga tcggcgccgc agcggcatcg ttgttgtctt gcgcacaaac
     3781 ggcaccgctg gcggcggcga gcaggctcaa cgcaccaatg cgcgcggaca gccacccggc
     3841 gcgggtgatc ttcataagca tttccccaac tcccgtatgc tgaaaattta ttgatcggcc
     3901 ttaaacgcca acattgcgcc ccgctttgcg cgagtcgcaa tcttcatatt tgaacgatca
     3961 aggcttcatc caaccggcgt ggaatgtagc aacattcgcg ccgcagagtc atccgcacga
     4021 gcaaaacacg tcaaccgcac ctgccctgag aatattatta tttatagtat atttttaatt
     4081 atattaaatt atttataaca atcaaaaccg ccttgcggtg gaggctgtac caacaaaaaa
     4141 cgggcgccgc agcgcccgct tttttgtcgc cagaattcgc ttacgcgctg gcagcggcag
     4201 catcagcctg cgcggcgggg cgcggacgat ccaccagttc cacgtacgcc atcggcgcct
     4261 tgtcgccggc acggaagccg cagcgcagga tgcgcgtgta gccgcccggc cgcgtggtgt
     4321 aacgcgggcc cagctcggtg aacagcttgg tcacggcgtc gcgatcacgc aggcggttga
     4381 aggcaatgcg gcggttggcc agcgtgtcct tcttggcgcg cgtgatcagc ggctccgcgg
     4441 cacggcgcag ttccttggcc ttggccacgg tcgtcttgat catctcgtgc ttgatcagcg
     4501 cattgaccac ggcctgcatg gtggccttgc ggtggctgct gctgcggccg aaagcgcggc
     4561 ccgaattggc gtgacgcatg ggagttccct agtgttgcgg ttgcttaggc aggcgtcttc
     4621 ggcgacgacc agttctcgag cttcatgccg agttccagat cgtggccctt gagcgcttcc
     4681 ttgatttcgt tgagcgactt cttgcccagg ttcggcgtct tcatcagctc ggtctcggtg
     4741 cgctgcacca ggtcgccgat gtaatagacg ttctcggcct tgaggcagtt ggccgaacgc
     4801 acggtgagct cgagatcgtc gatcgggcgg aacaggatcg gcgacaggtc cttcttgccc
     4861 ggggcctgtg cggccgcggc ttcagcctca aggtcgacga acacgccgag ctggtcgcgc
     4921 aggatgcgcg cggcaaggcg cacggcctcg gaggcatcga tgccgccgtt ggtgtccacg
     4981 tcgaggatca gcttgtccag gtcggtgcgc tgcgccacgc gcgcgcgctc caccgcgtac
     5041 gacacgcggc gcactgggct gtacgacgca tcgagcttga gcgcgccgat gctgtggccg
     5101 tcttcctcgc caggacgcgc ggtcgacagc gccggcacgt aaccgcggcc gcgcgtgacc
     5161 ttgagggtca tgttgagctt gccgccggtg aggtgcgcca gcaccaggtc cgggttgacg
     5221 atctcgacat cgtggtcgag cttgatgtcg gaagccttca ccggaccctt gccggacttc
     5281 tcgaggcgca gggtggcttc ctcgcgcgag tgcatgcgca cggcgatgtt tttgatgttg
     5341 agcaggatgt cgaccacgtc ttcctgtacg ccttcgatgg cgctgtactc gtgcaccacg
     5401 ccctcgatct gcacctcggt gatggcggcg ccggggatcg acgacagcag gatgcgacgc
     5461 agggcgttgc ccagcgtgtg gccgaagccg cgttccatcg gctccagcgc gatacgcgcg
     5521 cggttctcgg agacagcttc cacttcgatg gtgcggggct tgagcaagtc ggcaataacg
     5581 ttctgcatgt gtacctcggt aaaaaagccg cgcacccctc acggatgcgc tgtggtgtcg
     5641 cggatgcggc cggcgtcccg gccgcacgga gaacgcggtt atttcgagta caactcgacg
     5701 accaggttct cgttgatgtc cggcaggatc tggtcgcgcg tcggcagcgc cttgaacgtg
     5761 cccttgaggc ccttctcatc cacctcgacc cactcggcca ggccggcctg ggtggcgata
     5821 gacagcgact cggcgatgcg cgattggttg cgcgattttt cacgcacttc aatcacgtcg
     5881 ccgacctgca cctggtacga cgggatgttg accgacttgc cgttgaccat gatggccttg
     5941 tggcccacca gctggcggca ttccgaacgc gtgcggccga agcccatgcg gtaaatgacg
     6001 ttgtccagcc gcgcctcgag catcagcagc aggttgaggc cggtggcgcc cttgccgctc
     6061 gacgccgaca ggtagtagtt ccggaactgg cgctcgagta cgccgtacat ctgcttcagc
     6121 ttctgcttct cacgcaactg gccggcgtaa tccgacaggc gctgcttgcc ggcaccatgc
     6181 tgtccaggag gtgtatccag cttgcacttt gtctcgagcg agcgggcgcg gcttttcagc
     6241 agcaggtcgg cgccggcgcg gcgcgacagc ttgcacttgg gaccaatata acgagccatg
     6301 cttgtctctc tagatcagac gcggcggcgc ttgggagcgc ggcagccgtt gtgcggaatc
     6361 ggggtgacgt cggtgatgtt ggtgatcttc atgcccgcgg cattgagcgc gcgcaccgcg
     6421 gactcgcggc cggggccggg gcccttcacg cgcacttcca ggttcttcac gccatgctcg
     6481 gcagcagcgg tggcggcctt gtcggcggca acctgcgcgg caaacggcgt cgacttgcgc
     6541 gagcccttga agcccgccgc accggcggtg ctccacgaca gcgtattacc ctgccggtca
     6601 gtgatcagga tgatcgtgtt gttgaaagat gcgtgcacgt gggcaacggc atcggtcacg
     6661 ttcttcttga ccttcttgcg cttgggtgcg gcggcgccgg cgggtgcgtt tgctgcggga
     6721 ttagccataa atgctcggta ctaaaacttg atcagacggt cttcttgttg gtggccacgg
     6781 tgccgcggcg cggacccttg cgcgtgcgcg cattggtgcg cgtgcgctgg ccgcgcaccg
     6841 gcaggccgcg gcggtgacgc tggccgcggt agcagcccag gtccatcagg cgcttgatgc
     6901 tcatcgacac ttcgcggcgc aggtcgcctt ccaccgtgaa gcgcgccact tcggcgcgca
     6961 gcttgtccag ttcgccttcc gacagcgtgc ggatctgcac atcgggcttg atgccggcgg
     7021 cggcgcaaat cttcttggcg cgggtggcgc cgatgccgta gatcgcgcgc aaggcgatca
     7081 cggtgtgctt cttgtcggga atattgacgc cggcgatacg tgccatcgtg actttctcag
     7141 ttgcaaatcg cgcaaaaaag ggcgcggatt ttagtgaatt cctggggcgc tgacaaaccg
     7201 ggcgccttca gccctggcgc tgcttgtgac gcggatcgga gcagatcacg cggacaacgc
     7261 cgttgcgccg tatgaccttg cagttacggc agattttctt gacagatgcg cggaccttca
     7321 taaatgcctc gaactcaatt caggattacg gatcaggacg tcttggaacc gaccgcgcca
     7381 ccgctgcgca gattggcctt cttcatcagc ccttcgtact ggtgcgacat caggtgcgcc
     7441 tgcagctgcg ccatgaagtc catcaccacc accacggtga tcagcagcga agtgccgccg
     7501 aaataaaacg gcacatgcca ggtggtggtt aggaattccg gcgttaggca gaccacggtg
     7561 acataggccg cgccaatcac ggtcaggcgc accagcacct ggtcgatgta gcgggcggtc
     7621 atcgcgcccg gacgcacgcc cggaataaac gcacccgact tcttgaggtt ctccgcggtc
     7681 tcgcgcgagt cgaacaccag ggccgtataa aagaagcaga agaagatgat cagcgccgcg
     7741 tacacgatcg tgtacagcgc ctgacccggt gacaacgcgt tcgacagctg ctgcaggaat
     7801 ccgccactgc cttcctgacc gagaaagccg atgatcgtcg ccgggaacag aatgatcgac
     7861 gaggcgaaga tcggcgggat cacgcccgac atgttgagct tgagcggcag gtgctgggtc
     7921 tgcgcggcga acagcttgcg cccctgctgc cggcgcgcgt gatgaatcgg gatgcggcgc
     7981 tgcgcgcgct ccacgaacac cacgccccag gttacggcga tcgcgatggc gaacaccagg
     8041 atcacctgga aagcgttgat ttcaccgctg cccacgagct gcgcggtggc accgagtgcg
     8101 cgcggcatgt cggccacgat gccggagaag atcagcatgg acataccatt acccacgcca
     8161 cgctcggtga tctgctcgcc cagccacatc aggaacatgg tgcccgtcac cagcgaaatg
     8221 gtcgcggtga acatgaaacc gaaacccgga ttgagcacca cctgtgactt ggcctggaat
     8281 ccgtaagcca tgccgatgga ctggaacagc gcgagcccca gtgcgccata acgcgtgtac
     8341 ttggtgagcg tgcgccggcc ggaatcacct tccttgcgca actccttgag ctgcggaatg
     8401 accgccgtca gcaactgcac gatgatcgag gccgagatat acggcatcac gcccagcgca
     8461 aacaccgaca ggcgcttgaa cgagccgccc gagaacatgt tgaacatgtc caggatgctg
     8521 ccgccggtct gcttgttgaa cagcgccgcc agctggtccg ggttgatgcc cggcaccggg
     8581 atgaagctgc cgatgcggta gacgatgatc gcacccagca ggaacagcag gcgcgcacgc
     8641 acatccccga gcttcgccag atcgggaacc atggagcctg cagccgtggc gggagtcttg
     8701 gccattcttg ctctgggtta ggcgatcgaa ccgccggccg ccgcgaccgc ttcacgcgcg
     8761 cccttgctcg cgagtatgcc cttgagcacg actttgcggg tcaactcgcc ggacttgatc
     8821 agcttggctg tctgcgcgcg cggcgaaacc acgccttccg ctttcagcgt ggcgagatcg
     8881 atgtccgtgg ccttcatctt cgacaggtcc gacaggcgca cttcggcggt caagccggcc
     8941 gaaggcgaat tgaatccgcg cttgggcagg cggcgctgga tcggcatctg gccgccttcg
     9001 aaacccagct tgttgaagcc acccgaacgc gcgtgctgac ccttgtggcc gcggccggcg
     9061 gtcttgccga ggcccgaacc accaccgcgc gcgacgcgac gacgatcctt ctttgcgccc
     9121 ttagcgggct tgatgctgtt gagtttcatg tcaggcctgt tcgactttga gcatgtagga
     9181 aaccttgttc accaggccca ttatttccgg cgtgggctgc agcgtcacgg tctggtggcg
     9241 gcgcgacaac ccgaggccac gtacgcaatc ctggtgattg ggcaggcgct tggccaagct
     9301 gcgcatcagc gtcaccttga tttggccctt gctgtccttc ttggcagtca ttgcaatact
     9361 tccgattagg cgaggatttc ctcgaccgac ttgccgcgct tggcggcaat ctcggacggg
     9421 atgttcatct tcttcagcgc atcgatggtg gcgcgcacca tgttgatcgg attgcgcgag
     9481 ccaaaggcct tggccagcac gttctgcacg cccgcaactt cgaacacggc gcgcatggca
     9541 ccgccggcaa tgacgccggt accgtcagag gcgggacgca tgaacacgcg ggtcgagcca
     9601 tgcgtgcccc agatttcgtg ctgcagggtc aggcccttga gcggcacggt gatcatgttc
     9661 ttgcgcgcct tttccatcgc cttggaaatg gccaccggca cttcacgcgc cttgccatag
     9721 ccaaagccga cgcggccgga accatcgccg accacggtca gagcggtgaa gccgaactgg
     9781 cggccaccct tgaccacctt cgccacacgg ttgaccgcga ccagcttttc cttgaggtcg
     9841 ccgccggcct ggatgtcttc gacggggata ttcggtttag gcatcgctta aaactccagt
     9901 cctgcttcgc gcgcagcatc ggccagcgcc ttgatgcggc cgtgaaattt gaaacccgaa
     9961 cggtcgaatg cgactttggt cactccggcc gccttggcac gctcggcgat caacgcgcca
    10021 atcttcttgg cagcatcgac gttgccggta ccattgagac cgtcgatctc ctcgaccgtg
    10081 gatgccgcag cgaccgtggt gctagcgccc ggcgcaatga cctgcgcata aatatggcgc
    10141 ggagtgcgat gcaccgacag tcggtgcatg cccagttcgc ggatatgtgc gcgcgtacgg
    10201 cgggcgcggc gcaggcgtga gtcgttcttg tctttcatga tgcttaccg
//
LOCUS       DASZZC010000104         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_391194,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000104 DASZZC010000000
VERSION     DASZZC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391194"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VHE37_02505"
     CDS             complement(<1..500)
                     /locus_tag="VHE37_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HVT34428.1"
                     /translation="MRLDLNLINAELGRKPQELIAWVLSVRERPLITTNFGPYSPVLL
                     HQVTRQRPDIPVLWIDTGYSTPATYAFAHEVTRLLRLNLHVYHPLRSRAQRDAEEGAP
                     PALGDPRHAAFTQEVKLEPFARATHELAPDVWFTALRAEQTAERAAMQPVSVNKEGLM
                     KVAPLL"
     gene            575..1450
                     /locus_tag="VHE37_02510"
     CDS             575..1450
                     /locus_tag="VHE37_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34429.1"
                     /translation="MIPSSYGALARWQSSAGAGPVVRGRRASGDGDTIHFLSGNGFAG
                     GVYWPFLRLFLPRHALVTHDIEGHGDSDAAENYSGTGAVIARIPQVMADLQLPATPLI
                     GMGHSYGGALTLAVAARNPGLFRALVLLDPILLPWPAYWSMRMAALLHRHPMAESARR
                     RRDRWASRVEAFERLRGRGIYAGWSDEALNCFIDYATRDAADGTRVLCCPKGIEAQIF
                     EQPVYPWRLLPKIEVPVLFLRGAQSYGFFAWAERLAQRANPRVRVQQIAGGHCFMQQD
                     ASAAHAAVQEFLDSL"
     gene            complement(1505..2380)
                     /locus_tag="VHE37_02515"
     CDS             complement(1505..2380)
                     /locus_tag="VHE37_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34430.1"
                     /translation="MKSIKQVSSFCAMLVALAVLGACSANGDGSFGGGGSGAGSTGGT
                     TGGTTGGTTGGGDGTGPVTGGTTGGTTGGTTGGTTGGPPNDGSTGCTISDNGTVPASC
                     HFICTRSANLLYGATTETGLNGLVGASLTPLLNSLGGTTATQLLNSVTQPGQAIDGNL
                     DTYSTFALTAGLLGVIDSVDQTVHLPAVQPARSYAVFGVQFPSGTVDATLLSGVTVDT
                     LLGTTLQETMSATENQIDLLGATVTGDTNAFLGLHTTKAWDSARISMVPNLVSANVGD
                     AMHVNELCTEGAQLP"
     gene            complement(2377..3624)
                     /locus_tag="VHE37_02520"
     CDS             complement(2377..3624)
                     /locus_tag="VHE37_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT34431.1"
                     /translation="MKNAALPLLTALALVQAQAHALETQPLNSAPPDPSYGAEMSPAS
                     PAPAASPVEPVAPVAPAPSAAPAASENDDFWLFSSKGFSQNYLGLQIGYIYPNHHRGP
                     EVAKGLAVEGIYGWEGANHWGWEANVSHDTFDTGDNQGTDFYRYTFGADLTYSFGDRR
                     SFTPFLLVGLGGTHNDVVPDANDGFSYYGNAGVGFVTAPMIRALGDLRLRGEARWIYD
                     GYDHTPSGASADKIHGDWRFTLGIEIPLFAHHGYMTAANEDQVGVVPLAPESTPAACK
                     PVEAPTAYVDSAGCAIPRVLELKGVTFEFDKVRLRPDAETILNYVGDVIKRYPDMQVE
                     IAGHTDSMGSDGYNLKLSQRRAQAVVDYLVKHGVPTGQITAHGYGERQPIASNDTEEG
                     RERNRRVELRILNDVTPTTEPGK"
BASE COUNT          577 a         1315 c         1432 g          710 t
ORIGIN      
        1 tgcagcagcg gcgccacctt catcaggcct tccttgttca ccgacaccgg ctgcatcgcc
       61 gcgcgctcgg cggtctgctc ggcgcgcagc gcggtgaacc agacgtccgg cgccagttcg
      121 tgcgtggcgc gcgcaaacgg ctccagcttc acttcctggg tgaatgccgc gtggcgcgga
      181 tcgccgagcg cgggcggcgc gccttcctcg gcatcgcgct gcgcgcggct gcgcagcggg
      241 tgatacacgt gcaggttcag gcgcagcagg cgcgtcactt catgcgcgaa cgcgtacgtg
      301 gccggcgtcg agtagccggt gtcgatccac agcaccggga tgtccgggcg ctggcgtgtc
      361 acctggtgca gcagcaccgg cgagtaagga ccgaagttgg tggtgatcag cggccgctcg
      421 cgcaccgaca gcacccatgc gatcagctcc tgcggcttgc ggccgagttc ggcgttgatc
      481 aggttgaggt cgaggcgcat ggcgggcgcg gattataggg cgtgcggtta tcatcggccc
      541 gcattccggt ttttcaatcc cggtttttcc atccatgatc ccaagcagct atggcgcgct
      601 cgcgcgctgg cagtcgagcg cgggcgccgg tccggtggtg cgcgggcggc gcgcgagcgg
      661 cgacggcgac accattcact ttctctccgg caacggattc gccggcggcg tgtactggcc
      721 gttcctgcgg ctgttcctgc cgcgccacgc gctggtcacg catgacatcg agggccacgg
      781 cgacagcgac gcggcggaaa actacagcgg caccggcgcg gtcatcgcgc gcatcccgca
      841 ggtgatggcc gacctgcagc tgccggcaac cccgctgatc ggcatgggcc acagctacgg
      901 tggcgcgctc acgctggcgg tggcggcgcg caacccgggc ctgttccgcg cgctggtgct
      961 gctcgacccg atcctgctgc cgtggccggc ctactggagc atgcgcatgg cggcgctgct
     1021 tcatcgccac ccgatggccg agagcgcgcg gcggcggcgc gaccgctggg cctcgcgtgt
     1081 tgaagcattc gagcggctgc gcgggcgcgg catctatgcc ggctggagcg acgaggcgct
     1141 gaactgcttc atcgactacg ccacgcgcga cgccgccgac ggcacccgcg tgctgtgctg
     1201 tccgaaggga atcgaggcgc agatcttcga gcagccggtg tacccgtggc ggctgctgcc
     1261 gaagatcgag gtgccggtcc tgttcctgcg cggcgcgcag agctatggat tcttcgcctg
     1321 ggcggaacgg ctggcgcagc gcgccaaccc gcgcgtgcgc gtgcagcaga tcgccggcgg
     1381 ccattgcttc atgcagcagg atgcatcggc cgcgcatgcg gcggtgcagg aatttctcga
     1441 ctcgctctag cgcgcaaaaa aaaccgccgg cactgggccg gcggtcgtgg gtgccgcagc
     1501 tgcctcaggg cagttgcgcg ccctcggtgc acagctcgtt cacgtgcatg gcgtcgccga
     1561 cgttggccga caccaggttg ggcaccatcg agatgcgcgc gctgtcccag gccttggtgg
     1621 tgtgcaggcc gaggaaggcg ttggtgtcgc cggtgacggt ggcgccgagc aggtcgatct
     1681 ggttctcggt ggccgacatg gtttcctgca gcgtggtgcc gagcagcgtg tcgacggtga
     1741 cgccgctgag cagcgtcgca tccaccgtcc ccgacgggaa ctgcacgccg aacacggcgt
     1801 agctgcgcgc cggctgcacc gccggcaggt gcaccgtctg gtccaccgaa tcgatcacgc
     1861 cgagcaggcc ggcggtgagc gcgaaggtgg agtaggtgtc gagattgccg tcgatggcct
     1921 ggcccggctg cgtcacgctg ttgagcagct gcgtggcggt ggtgccgccc agcgagttga
     1981 gcagcggcgt gagcgacgcg cccaccagcc cgttgaggcc ggtctcggtg gtggcgccgt
     2041 acagcaggtt ggcgctgcgc gtgcagatga agtggcagct ggccggcacg gtgccgttgt
     2101 cggagatggt gcagccggtg ctgccgtcgt tgggcgggcc gccggtggtt ccgccggtcg
     2161 ttcctcccgt ggtgccgccc gtggtgccgc ccgtgaccgg gccggtgcca tcgccgccgc
     2221 cggtggtgcc gccggtcgta ccgccggtgg tgccacccgt cgagccggcg ccagagccgc
     2281 cgccgccgaa gctgccgtcg ccgttggccg agcaggcgcc gagtaccgcg agggccacca
     2341 gcatggcgca gaaggaagaa acttgcttga tcgatttcat ttgccaggtt ccgtagtcgg
     2401 agttacgtcg ttgaggatgc gcagttcgac gcggcggtta cgctcgcgcc cttcttcggt
     2461 gtcattgctc gcgatcggtt gacgctcgcc gtagccatgc gcggtgatct ggccggtcgg
     2521 cacgccgtgc ttgaccaggt aatccaccac ggcctgcgcg cgtcgctgcg acagcttcag
     2581 gttgtagccg tcgctgccca tgctgtcggt gtggccggcg atctccacct gcatgtcggg
     2641 ataacgcttg atcacgtcgc cgacgtaatt gaggatggtt tccgcatcgg gccgcaggcg
     2701 caccttgtcg aactcgaagg tcacgccctt gagctccagc acgcgcggga tggcgcaacc
     2761 ggcggaatcg acataggccg tcggcgcctc gaccggcttg cacgcggccg gcgtgctttc
     2821 cggcgcgagc ggcaccacgc ccacctggtc ctcgttggcc gcggtcatgt acccatggtg
     2881 cgcgaacagc gggatctcga tgcccagggt gaagcgccag tcgccgtgga tcttgtcggc
     2941 cgacgcgccg ctgggcgtgt ggtcgtagcc gtcgtagatc cagcgcgcct cgccgcgcag
     3001 gcgcaggtcg cccagcgccc ggatcatcgg cgcggtgacg aagcccacgc ccgcgttgcc
     3061 gtagtaggag aagccgtcgt tggcgtcggg caccacgtcg ttgtgcgtgc cgcccaggcc
     3121 gaccagcagg aacggtgtga agctgcggcg gtcgccgaac gagtaggtga gatcggcgcc
     3181 gaaggtgtag cggtagaaat cggtgccctg gttgtcgccg gtgtcgaaag tgtcgtgcga
     3241 cacgttggct tcccagcccc agtggttggc gccttcccag ccgtaaatgc cttccaccgc
     3301 caggcccttg gccacttccg ggccgcggtg atggttggga tagatgtagc cgatctgcag
     3361 gccgagatag ttctgcgaga agcccttgga cgagaacagc cagaaatcgt cgttctccga
     3421 cgcggccggg gcggctgacg gcgcgggcgc caccggcgcg accggctcaa ccggcgatgc
     3481 ggccggtgcc ggcgacgccg gcgacatctc ggcgccgtag ctcgggtcgg gcggagcgga
     3541 gttcagcggc tgggtttcca gcgcatgtgc ctgggcctgt accagcgcca gggcggtcaa
     3601 caacggcaac gcggcattct tcattctgat ttccccgtcc agttcttgaa aagcgaagct
     3661 tgattcgtgg gtattaactc gtggttaagc gcccggcggc gttcttgtaa acagaaagtt
     3721 tgcaaaaggc aacccgccag atatccatgg catccaatgt ctgtcgtttg cctctctccc
     3781 acccgcataa attactgtag tcaggcttcc cgcgctgctg ggaaagttcc ctctggcttg
     3841 tcaggcttcg atgacgtggc gcctgtcatg tctggcagtc tgcgaccttt ttccatcctg
     3901 gcgcatccaa gcgacatgac cgccaccccg tctggattcg atgcaagtat cgcacccgag
     3961 ctgatggccg acgcccgtgc ggggggcgcg cgcggcaggg aggcgatcta ccgggccttt
     4021 gagcggccgg tcta
//
LOCUS       DASZZC010000105        10760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_266690,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000105 DASZZC010000000
VERSION     DASZZC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266690"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VHE37_02525"
     CDS             complement(<1..285)
                     /locus_tag="VHE37_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVT34432.1"
                     /translation="MFKPLIDWYLAQLATGGYPVIVLLMAIESSFLPLPSELVIPPAA
                     ILAARGDSGLSYLGIIIASALGSLIGASLMYWGSRWIGRPLVARYGRWIMI"
     gene            complement(376..2121)
                     /locus_tag="VHE37_02530"
     CDS             complement(376..2121)
                     /locus_tag="VHE37_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT34433.1"
                     /translation="MATYKAPLSEINFVLNDVLDVGQLAQLPALKDATPDTLAGLVEE
                     AARLIEEKLAPLNMAGDSGCKLKDGEVTPPAGFKEIYELWSKGGWMSIAAKAEYGGQG
                     LPYTLSKVLEEMVCSANVAFALYPGLTTGCYEAIEACGNEEQHQTYLPKLASGEWSGT
                     MCITEPQAGSDVGAAKTKATPQADGSYLIDGGKIFISSGEHNLAPNIIHFVLARLPDS
                     PPGVKGLSTFVVPKFLVNKDGSLGARNALRCVSIEHKMGLHGSCTCTMAFEGARGWMV
                     GQPNQGIQNMFVMMNLARIMVGYQGLGQCELATQNAIRYAQERKQGKAFNGKQQIIEH
                     PDVRRMLLQMKATTEGARMLAYETAIYVDLARHATDPAVREEAQDFVDLNTPLVKSFC
                     TDSAFELGSQAIQVYGGHGFIKEHGIEQIARDSKILCLYEGTNGIQAMDLVRRKLMMN
                     GGALPKRFFAKARKHINAAGKDYAYIAQPLAKVIDDLEATTQWIQQSYKTTPDDAAFG
                     CVDFQRAFCLAYLGLNWLRMAQAAATKSEAALRDGKRATALYFAQRMLPQVHSLLANV
                     RNPAAELMALDAANF"
     gene            2366..2704
                     /gene="yajC"
                     /locus_tag="VHE37_02535"
     CDS             2366..2704
                     /gene="yajC"
                     /locus_tag="VHE37_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HVT34434.1"
                     /translation="MLDFFLSPAYAQGTQPVSPMAQFLGGPGFIIVMLVVMYFMLIRP
                     QMKRGKEQRQMLGALAKGDEVLTNGGMAGKLIAIGENYVTLEVADNIAVKVQKQAIAS
                     VLPKGTLKNL"
     gene            2793..4649
                     /gene="secD"
                     /locus_tag="VHE37_02540"
     CDS             2793..4649
                     /gene="secD"
                     /locus_tag="VHE37_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVT34435.1"
                     /translation="MHPYPKWKYVALLIAIVLGVLYAAPNLYGEQPAVQISLESGEPL
                     TPAFGEQVSKALATDKIVSAGGSIETKGWIERFDDETAQLKAVDTLKRELGHGYVVAL
                     NLAPKTPGWLRSFGAKPMALGLDLRGGVHFLLEVDVNDVRKKTITRLVTDLPAFLRRQ
                     DIHYSGRREAGDAVVLEFPDADKLAAARKAIAKEFPELTLVTPDAGPLTIEARLSDSE
                     LKNIQQRAVEQNLITLRNRVNQLGVAEPLVQRQGAERIVVEMPGVQDTAQAKEVLGST
                     ATLEYRAVDEGDPNAAASSGIVPAGPELFYTRDTHRPVLLKKDIIAAGDQLIDAASTV
                     DENGQPAVSVTLDGSGAKKMFEFTSKNVGKPMAVLFRERVTDTTYNAKGEAMRSHRDV
                     EDVISVATIRGVFSKRFQTTGLSTKEAHDLSLLLKAGALAAPIDIVEERTIGPSLGAD
                     NIRRGFQAAALGLALVVGFMLLYYRVFGLFAIAALTLNLLLVIAELSLVQATLTMPGI
                     AGIVLTMGIAVDANVLIYERIREDLRNGMKPHAAIEAGYDRAFLTIADANVTVLIGTI
                     VLFALGAGPVKGFAVTLALGIITSQFTAIVGSRALAQLVFEKRKLTNDLPIW"
     gene            4660..5607
                     /gene="secF"
                     /locus_tag="VHE37_02545"
     CDS             4660..5607
                     /gene="secF"
                     /locus_tag="VHE37_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVT34436.1"
                     /translation="MKFFARVPNIDFMAQRKVGLIVSSVLTAASILLVLFRGLNLGID
                     FSGGVLVEVVYPQTVQLDTVRSALEQGGYPRAIVQYFGGTSDVLIRLPPAENASTAQV
                     SGEVLKALGATDACEDGATQPCKRRIEFVGPQVGEELTSQGGTAMLMAFIGIMIYIAF
                     RFEWKFAVGAIAALIHDALMVVGFLSVTQVEFDLTTLAAILALIGYSNNETIVIFDRV
                     RENFRELRREGTLEVVNLSVNQTLLRSVITHLTTLLVLTALFFLGGHSVHSFAIALMV
                     GVVVATYSSIYVSTGLAVAMGVSREDLLPPKQEEIDDLP"
     gene            5660..5794
                     /locus_tag="VHE37_02550"
     CDS             5660..5794
                     /locus_tag="VHE37_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34437.1"
                     /translation="MEFASRPGQIHQIGTVAALNMRAADESGGESLMVAANAVATVNQ
                     "
     gene            5850..7037
                     /locus_tag="VHE37_02555"
     CDS             5850..7037
                     /locus_tag="VHE37_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT34438.1"
                     /translation="MNQFVEVFGFAAFALLTSYWVCRMVIGAAHRLDIGTDELAGVQK
                     MHRHRVSRLGGVPIFLAFLSSLLFLSWTSGNFKVGTAFLVVCMLPAFGFGLVEDLTRR
                     VGATERLIAAMISGALGWWLFSAGLVRIGWEGADIFLETHPWAVLVMTVMAASGSAHG
                     VNIIDGCNGLSGFFIMSALAALMVVAAGARDMFVFQCALLALASTAGFFAWNFPNGRI
                     FLGDSGAYMMGFLIAQLAMILFTRHPEVSPWCAMLIMAYPAWETLFSMYRRSRHGLRQ
                     LGKPDARHLHHLVYRRILKWAPESAPQRVRDFRSAFTSVLVWPLMLLCMMPAAMFWYD
                     TKKLFLLCWLFALTYTAMYVCIARFKVAAPITRLVTRIKRRVEQAEVVPAPVPPAAAR
                     VVQ"
     gene            complement(7068..8075)
                     /gene="galE"
                     /locus_tag="VHE37_02560"
     CDS             complement(7068..8075)
                     /gene="galE"
                     /locus_tag="VHE37_02560"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HVT34439.1"
                     /translation="MSHRILIVGGAGYIGSHMTLCLQAHGLEVTVLDNLSTGSRELVH
                     TPDLVIGDMADDNLVSGVIRSRGIAAVMHFASCINVGESVRDPGKYYENNVGKTISLL
                     NTIVRCGIRRMIFSSTSAVYGTPERLPINEQAPLAPLNPYGESKAWVERVLAAFDHAH
                     GLRSYSLRYFNAAGADPQGRAGECHDPETHLIPIALQVALGQREQLDILGDDYRTEDG
                     TGVRDYVHVTDLCEAHYLALLDLFNGGAGGILNVGIGRGFTVRQVVAAVEKVTGRPVP
                     TRIQPRRPGDTDALVADSRNIQDRLGWRPRYTDLETIVRHAWAWEKNGRRTAPAERGD
                     T"
     gene            complement(8121..9332)
                     /locus_tag="VHE37_02565"
     CDS             complement(8121..9332)
                     /locus_tag="VHE37_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HVT34440.1"
                     /translation="MKTNPNSLGVGLGIIIATGLAACSSVPSSGPSTSEVLDSARTNS
                     DSVAMLQNQGISYLITDINPHVVAALKNQLTPSLRAAFGDGTSDVQGTLRPGDRVTLT
                     IWESGPGALFSPSASTVSSVPTMAQTSVIPEQTIAPDGTILVPYAGRVKVAGMTGPEA
                     QMRIEEKLHGRTSQPQVLLTVSLSMSDVVTIAGDNAKGGLVPLATAGSDRILDVIAQT
                     GGITSPVYAATVQLIRSGKSATVPLQNVILNPDENIRVHPGDTLVISRNPEYFTALGA
                     TGKSAQVPFEAADVSLTEALGKAGGLLDERADPSGVFVFRYETRDTALRLCPSCEFVP
                     DQVQVPVVYRLDAREPRNLFSAQSFAMTNRDLLYVSNAPPVPLQKVLGIIRDFLSPVL
                     TGAVVDRAVNK"
     gene            complement(9345..9752)
                     /locus_tag="VHE37_02570"
     CDS             complement(9345..9752)
                     /locus_tag="VHE37_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34441.1"
                     /translation="MELIIGRGLMPFLRGVAVFLAIRYLTGSSISGLAVGLIPFLLGV
                     LGVGGRISAAVTALSIVAALCWLATPESTRTVLRSFASATFDDHLPAAGTETAGIPTQ
                     TPTGSMPQATPQASPGAVPPAIPPSPSQASKPH"
     gene            complement(9805..>10760)
                     /locus_tag="VHE37_02575"
     CDS             complement(9805..>10760)
                     /locus_tag="VHE37_02575"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HVT34442.1"
                     /translation="ICVSGSSVPAPSIQRWLKESPNVSGIFMLHDLIPVTHPEYARPK
                     MAARHRKYVEDLTRCASAVITNSQFTAESLSRFAADHGLKVPRTVVAPLGISPHFTRT
                     QPKADLAPYFVFVGTIEPRKNHILLLQVWQRLVVQHGPAAPKLVIVGRRGWENENVLD
                     LLDRGENLRSHVIECNQLCDELLIELIANARALLLPSHVEGYGLPVAEALALKTPVIC
                     SDLPPLREIAGDVPEYVDQLAGRGWARTIMDYADLQNPRRRAQVERMARFQAPLWKDH
                     FRKIEDAMPNIGLCAEDGVPAAAQLVNLGASAPLAETAAVK"
BASE COUNT         1825 a         3352 c         3592 g         1991 t
ORIGIN      
        1 gatcatgatc cagcggccgt agcgtgcgac cagcgggcgg ccgatccagc gcgagcccca
       61 gtacatcagg ctggcgccga tcagcgagcc gagcgcgctg gcgatgatga tgcccaggta
      121 ggaaagcccg ctgtcgccgc gcgcggcgag gatggcggca ggcggaatga ccagttcgct
      181 gggcagcggc aggaagctgc tctcgatggc catcagcagc acgatcaccg ggtagccgcc
      241 ggtggccagc tgcgccaggt accagtcgat caggggcttg aacatggatt tcttgtcatc
      301 ccgggcgcgg cgagggattt catcatcgct gcgttttggc ggagggtgag attcctcact
      361 tcgttcggaa tgacattaaa aattcgcggc atccagcgcc atcagttccg ccgccgggtt
      421 gcgcacgttg gccagcaggc tgtgcacctg cggcagcatg cgctgggcga agtacagcgc
      481 ggtggcgcgc ttgccgtcgc gcagcgctgc ttcggatttc gtagcagcgg cctgcgccat
      541 gcgcagccag ttgaggccga ggtaggccag gcagaaggcg cgctggaaat cgacgcagcc
      601 gaaggcggcg tcgtccggcg tggtcttgta cgactgctgg atccactgcg tggtcgcttc
      661 cagatcgtcg atcaccttgg ccagtggctg ggcgatgtac gcgtaatcct tgccggcggc
      721 attgatgtgc ttgcgtgcct tggcgaagaa gcgcttgggc agcgcgccgc cgttcatcat
      781 cagcttgcgc cgcaccaggt ccatggcctg gatgccgttg gtgccttcgt acaggcacag
      841 gattttcgag tcgcgcgcaa tctgctcgat gccgtgttcc ttgatgaagc cgtggccgcc
      901 gtagacctgg atcgcctggc tgccgagctc gaacgcgctg tcggtgcaga acgatttcac
      961 cagcggcgtg ttgaggtcga cgaaatcctg tgcctcttcg cgtaccgccg ggtcggtggc
     1021 atggcgcgcg aggtcgacgt agatcgcggt ctcgtaggcg agcatgcgcg caccttcggt
     1081 ggtggccttc atctgcagca acatgcggcg cacgtcggga tgctcgatga tctgctgctt
     1141 gccgttgaac gccttgccct gcttgcgctc ctgcgcgtag cggatggcgt tctgcgtggc
     1201 cagttcgcac tggcccaggc cctggtagcc caccatgatg cgcgccaggt tcatcatcac
     1261 gaacatgttc tggatgccct ggttgggctg gccgaccatc cagccgcgcg cgccctcgaa
     1321 ggccatggtg caggtgcacg agccatgcag gcccatcttg tgctcgatcg acacgcagcg
     1381 cagcgcgttg cgcgcgccga gcgagccatc cttgttcacc aggaatttcg gcacgacgaa
     1441 ggtggacagc cccttcacgc ccggcggcga gtcgggcagg cgcgccagca cgaagtggat
     1501 gatgttcggc gccaggttgt gctcgccgga gctgatgaag atcttgccgc cgtcgatgag
     1561 atagctgccg tcggcctgcg gcgtggcttt ggtttttgcg gcgccgacat cggagccggc
     1621 ctgcggctcg gtgatgcaca tggtgccgct ccactcgccg gacgcgagct tgggcagata
     1681 ggtttggtgc tgttcttcgt tgccgcaggc ttcgatggcc tcgtagcagc cggtagtcag
     1741 gccggggtag agcgcgaacg caacattcgc cgagcacacc atttcctcca gcaccttgct
     1801 cagcgtgtag ggcaggccct ggccgccgta ctcggccttg gcggcgatgc tcatccagcc
     1861 gcccttcgac cacagttcgt agatttcctt gaaacccgcg ggcggcgtga cttcaccgtc
     1921 cttcaacttg catccgctgt cgcctgccat gttgagcggc gcgagttttt cctcgatcag
     1981 cctggcggct tcctcgacca ggccggcgag cgtgtcgggc gtggcgtcct tcagtgccgg
     2041 cagctgcgcc agctggccca cgtcgagcac atcattcagg acaaagttga tctcggacag
     2101 cggcgccttg tacgtggcca tggatgcacg cgcggaaaaa atggggcgcg caggttagca
     2161 cgcgttgcgc agcgagttga gcagccgatg aggaatgcgc agggtgccaa aaatggggtc
     2221 accgcaccgc tgttccgact gttataaata atcatataaa attacaaacg taagatattt
     2281 aataacatga aaaagcgggg tgcggtgagg ctgctgtggc ataatcgcgc gctcaaaaaa
     2341 tggcccccag ggctggagta aaaccgtgtt ggatttcttc ctgagcccgg cgtacgcgca
     2401 gggcacgcag ccggtcagcc cgatggcgca gttcctcggc gggcccggct tcatcatcgt
     2461 catgctggtg gtgatgtact tcatgctgat ccgtccgcag atgaagcgcg gcaaggaaca
     2521 gcgccagatg ctcggcgcgc tggccaaggg cgacgaggtg ctgaccaacg gcggcatggc
     2581 cggcaagctc atcgccatcg gcgagaacta cgtgacgctg gaagtggcgg acaacatcgc
     2641 ggtaaaggtg cagaagcagg ccatcgccag cgtgctgccc aagggcacgt tgaaaaatct
     2701 gtaggagcgg gccttgcccg cgatgaggtc gtcctcatcg tgcatcgcgc gcaaggcgca
     2761 ctcctacacg acaagaaatc gagagaacgc ggatgcatcc ttatccgaag tggaaatacg
     2821 ttgcgctgct catcgccatc gtgctcggcg tgctgtacgc cgcgcccaat ctctacggcg
     2881 agcagccggc tgtgcagatc tcgctggagt caggcgagcc gctgacgccg gccttcggcg
     2941 agcaggtgag caaggcgctg gccaccgaca agatcgtctc ggccggcggc agcatcgaga
     3001 ccaagggctg gatcgaacgc ttcgatgacg agaccgcgca gctgaaggcg gtggatacgc
     3061 tcaagcgcga actcggccac ggctacgtgg tggcgttgaa cctggcaccc aagacgccgg
     3121 gctggctgcg cagctttggc gccaagccca tggcgctggg cctggacctg cgcggcggcg
     3181 tgcacttcct gctcgaagtc gacgtgaacg acgtgcgcaa gaagaccatc acccggctgg
     3241 tcaccgacct gccggcgttc ctgcgacgcc aggacatcca ttacagcggc cgccgcgaag
     3301 cgggcgacgc ggtggtgctc gagtttcccg atgccgacaa gctggccgct gcgcgcaagg
     3361 ccatcgccaa ggaatttccc gagctgacac tggtcacgcc ggatgcgggg ccgctgacca
     3421 tcgaggcgcg cctgtccgac tccgagctga agaacatcca gcagcgcgcg gtggagcaga
     3481 acctgatcac gctgcgcaac cgcgtcaacc agctgggcgt ggccgagccg ctggtgcagc
     3541 gccagggcgc cgagcgcatc gtggtggaga tgccgggcgt gcaggacacc gcgcaggcca
     3601 aggaagtgct gggcagcacc gccacgctgg aataccgcgc agtggacgag ggcgatccga
     3661 atgctgccgc ctccagcggc atcgtgccgg ccggccccga actgttctac acacgcgaca
     3721 cgcaccggcc ggtgctgctg aagaaggaca tcatcgccgc cggtgaccag ctgatcgacg
     3781 cggcgtccac cgtggacgag aacggccagc cggcggtgag cgtgacgctc gacggcagtg
     3841 gcgccaagaa gatgttcgag ttcaccagca agaatgtcgg caagccgatg gccgtgctgt
     3901 tccgcgagcg cgtcaccgat accacctaca acgccaaggg cgaagccatg cgctcgcacc
     3961 gtgacgtgga ggacgtgatc tccgtcgcca ccatccgcgg cgtgttctcc aagcgcttcc
     4021 agaccaccgg cctgtcgacc aaggaagcgc acgacctgtc gctgctgctc aaggccggcg
     4081 cgctggcggc gcctattgac attgtcgagg agcgcaccat tggcccctcg ctgggcgccg
     4141 acaacatccg ccgcggcttc caggcggcgg cgctgggcct ggcgctggtg gtcggcttca
     4201 tgctgctcta ctaccgcgtg ttcggcctgt tcgccatcgc cgcgctgacg ctgaacctgc
     4261 tgctggtgat cgccgagctg tcgctggtgc aggcgacgct gaccatgccg ggcatcgccg
     4321 gcatcgtgct gaccatgggt atcgcagtgg acgccaacgt gctgatctac gagcgcatcc
     4381 gcgaggatct gcgcaacggc atgaagccgc acgcggcgat cgaggccggc tacgaccgcg
     4441 ccttcctgac catcgccgac gccaacgtca ccgtgctgat cggcaccatc gtgttgttcg
     4501 ccctcggggc aggcccggtg aaaggcttcg ccgtcacgct ggcgcttggc atcatcactt
     4561 ctcagttcac cgccatcgtc ggctcgcgcg cgctggcgca gctggtgttc gagaagcgca
     4621 agctgaccaa cgacctgccg atctggtgag ggcacgacca tgaaattctt tgcacgcgtt
     4681 cccaacatcg atttcatggc ccagcgcaag gtgggcctga tcgtgtcgtc ggtgctcacc
     4741 gcggcctcga tcctgctggt gctgttccgc gggctgaacc tgggcatcga cttctccggc
     4801 ggcgtgctgg tggaagtggt ctacccacag accgtgcagc tcgataccgt gcgcagcgcg
     4861 ctggagcagg gcggctatcc gcgcgccatc gtgcagtact tcggcggcac gtccgatgtg
     4921 ctgatccgcc tgccgcccgc ggaaaatgcc agcaccgcgc aggtcagcgg cgaggtgctc
     4981 aaggcgctcg gtgccaccga tgcctgcgag gacggcgcta cgcagccgtg caagcggcgc
     5041 atcgagttcg tcggcccgca ggtgggcgag gagctgacca gccagggcgg cacggccatg
     5101 ctgatggcct tcatcggcat catgatctat atcgccttcc ggtttgaatg gaagttcgcg
     5161 gtgggcgcca ttgccgcact gatccacgac gcgttgatgg tggtgggatt cctgtcggtc
     5221 acccaggtcg agttcgatct caccacgctg gccgcgatcc tcgcgctgat cggctactcc
     5281 aacaacgaaa ccatcgtcat cttcgaccgc gtgcgcgaga acttccgcga actgcgccgg
     5341 gagggcacgc tcgaagtggt gaacctgtcg gtcaaccaga cgctgctgcg ctcggtcatc
     5401 acgcacttga ccacgttgct ggtgctcacg gcgctattct tcctgggtgg ccactcggtg
     5461 cacagctttg ccatcgcgct gatggtgggc gtggtggtgg cgacgtattc atcgatctac
     5521 gtgtcgaccg gcctggccgt ggccatgggc gtgtcgcgcg aggacctgct gccgcccaag
     5581 caggaggaga tcgacgacct gccttgagac ctgcgtcacg gtctgagggc agccagcagc
     5641 gaccagtatc aaatagacca tggaatttgc cagccgcccc gggcaaattc atcaaattgg
     5701 cacggttgcc gcattgaata tgcgcgcggc tgacgaaagc ggtggggaaa gtctgatggt
     5761 agccgccaat gcagtggcta cagtaaacca gtagaaccgg acttggcggc gcagaccgac
     5821 ggtccgggtc gcggcagagg agccgacagg tgaatcagtt tgtggaagtg ttcgggtttg
     5881 cggcgttcgc gctgctgacc agctattggg tctgccgcat ggtgatcggg gcggcgcacc
     5941 ggctggacat cggtaccgat gaactcgccg gcgtacagaa gatgcaccgc caccgcgtgt
     6001 cgcgcctggg tggtgtgccc attttcctgg ctttcctctc gtccttgctg ttcctgtcct
     6061 ggaccagcgg caacttcaag gtggggacgg ccttcctggt ggtctgcatg ctgccggcgt
     6121 tcggcttcgg cctggtggag gatctcaccc gccgcgtcgg ggccaccgaa cggctgatcg
     6181 ccgccatgat ttcaggtgct ctgggctggt ggctgttttc cgcggggctg gtgcgcatcg
     6241 gctgggaagg cgccgacatc ttcctggaga cgcatccctg ggccgtgctg gtcatgacgg
     6301 tgatggcggc gtccggctcg gcgcacggcg tgaacatcat cgatggctgc aacggcctgt
     6361 ccggcttctt catcatgagc gcactggcgg ccctgatggt ggtggcagcc ggcgcgcgcg
     6421 acatgttcgt tttccagtgc gccttgctgg cgctggcgtc gaccgcgggc ttcttcgcct
     6481 ggaacttccc caacggccgc atcttcctgg gcgattccgg cgcctacatg atgggctttc
     6541 tcatcgccca gctggccatg atcctgttta cccgccaccc ggaagtttcc ccgtggtgcg
     6601 cgatgctgat catggcgtac ccggcctggg aaaccctgtt ctcgatgtac cgccgctccc
     6661 gccacggctt gcgccagctg ggcaagcccg acgcacgcca cctgcatcac ctggtctacc
     6721 ggcgcatcct caagtgggcg ccggagtcgg cgccgcagcg cgtgcgcgat ttccgcagcg
     6781 ccttcacctc ggtgctggtc tggccgctga tgctgttgtg catgatgccg gcggccatgt
     6841 tctggtacga cacgaagaaa ttgttcctgc tgtgctggct gttcgcactg acctacacgg
     6901 cgatgtacgt ctgcatagcg cgcttcaagg tggcggcgcc gatcacccgg ctggtgacgc
     6961 gaatcaagcg gcgtgtcgag caggccgaag tcgtgcctgc cccggtgccg ccggcggctg
     7021 cccgcgtcgt ccagtgaccc ggacgcttag cgaggcctga ttcgacttca ggtatcgccg
     7081 cgttctgccg gcgcagttct gcggccgttc ttttcccacg cccaggcgtg gcgcacgatg
     7141 gtttccaggt cggtgtagcg cggacgccag cccaggcgat cctggatgtt gcgggagtcc
     7201 gccaccagcg cgtcggtgtc gcccggccgg cgcggctgga tgcgcgtggg gaccggccgg
     7261 ccggtcactt tctcgactgc ggcaacgacc tgccggacgg tgaatccgcg tccaatgccc
     7321 acgttgagaa ttccgcccgc gccgccattg aacaggtcca gcagcgcaag gtagtgcgcc
     7381 tcgcacaggt cggtgacgtg cacgtagtcg cgtacgccgg ttccgtcctc ggtacgatag
     7441 tcgtcgccga ggatgtccag ctgctcgcgc tgtcccagcg ccacctgcag ggcaatgggg
     7501 atcaggtgcg tttccgggtc gtggcattcc cccgcgcggc cttgcggatc ggcgcctgcc
     7561 gcgttgaagt agcgcaggct gtacgagcgc aacccatgcg cgtgatcgaa tgcggcgagt
     7621 acgcgttcga cccaggcctt gctttctccg tacggattga gcggagccag cggcgcctgc
     7681 tcgttgatcg gaaggcgttc aggcgtcccg taaacggcgc tggtcgatga aaaaatcatg
     7741 cggcggatgc cgcaacgcac gatggtgttc agcagcgaga tggtcttgcc gacattgttc
     7801 tcgtaatact tgccgggatc gcgtaccgat tcgccgacgt tgatgcagga ggcgaagtgc
     7861 atgaccgccg caatgccgcg gctgcggatg acgccggata ccaggttatc gtcggccata
     7921 tcgccgatca ccaggtccgg tgtgtgcacc agttcgcgcg agccggtgga cagattgtcg
     7981 agaaccgtca cttcgaggcc atgcgcctgc aggcacaggg tcatgtgtga gccgatatag
     8041 cccgctccgc cgacgatgag gatgcggtgg gacatggact tgctgtcggg agaccttttt
     8101 gcgcctgacg agccgcgcgg ctacttgttg acggcgcggt cgaccactgc cccggtcagc
     8161 accggcgaca ggaagtcgcg gatgatgccg agcaccttct gcagcggcac cggcggggca
     8221 ttcgagacgt aaagcaggtc gcggttggtc atcgcaaacg actgcgcgct gaacaggttg
     8281 cgcggctcgc gggcatccag ccggtagacc accgggacct ggacctgatc ggggacgaat
     8341 tcgcacgacg ggcacagccg cagcgcggta tcgcgggtct cgtaacggaa aacgaagacg
     8401 cccgaaggat ctgcgcgctc gtcgagcagg ccgccggcct tgcccagcgc ttcggtcagg
     8461 ctgacgtcag ccgcttcgaa cggcacctgc gcgctcttgc cggtggcgcc gagggcggtg
     8521 aagtattccg ggttcctgga gatgaccagc gtgtcgccgg gatgcacgcg gatgttctcg
     8581 tcgggattga gaatcacgtt ctgcagcggc accgttgccg atttgccgga acggatcagc
     8641 tgcacggtcg ccgcgtacac cgggctggtg atgccgccgg tctgcgcgat gacgtcgaga
     8701 atgcggtcgc tgcccgccgt cgcgagcgga accagcccgc ccttggcgtt gtcgccggcg
     8761 atggtgacca cgtccgacat gctcagtgac acggtaagca ggacctgcgg ctgcgaggtg
     8821 cggccgtgaa gtttttcctc gatgcgcatc tgcgcttccg gcccggtcat gccggccact
     8881 ttcacgcggc ccgcatacgg caccaggatg gtgccgtcag gcgcgatggt ctgctcgggg
     8941 atcacgctgg tctgcgccat ggttggaaca ctggacacag tgctcgccga cgggctgaac
     9001 agcgcgcccg gtcccgattc ccagatggtc agggtaacgc ggtcgccggg gcgcagcgtc
     9061 ccctgcacgt cagacgtgcc gtcgccaaaa gccgcgcgca ggctgggcgt gagctggttc
     9121 ttcagcgcgg cgaccacgtg tggattgata tcggtgatca ggtagctgat gccctggttc
     9181 tgcagcatcg caacggaatc ggaattcgtg cgagcgctgt cgagaacttc cgaggtgctt
     9241 ggcccggaag aagggacgct gctacaggcg gccagaccgg tcgcgataat aatgcccagg
     9301 ccgacgccaa gtgaattcgg attcgttttc atggtcgcat tccttcagtg tggtttgctt
     9361 gcctgcgaag gtgatggagg gattgccggt ggcacggccc cagggctggc ctggggagtt
     9421 gcttgcggca tcgatcctgt aggcgtttgc gtcgggatgc cagctgtctc agtgcctgca
     9481 gcaggcaggt gatcgtcgaa ggttgcggat gcaaagctgc gcagcacggt ccgtgtactc
     9541 tccggagtgg caagccagca gagtgctgcc acgatactca gcgccgtcac cgcggcagag
     9601 atgcggcctc caacacccag cacccccaac aagaatggaa tcaagcccac ggcgagcccg
     9661 gagatgctgc tgccggtgag atagcggatg gccaagaaaa ctgcgacgcc gcgtagaaag
     9721 ggcatcaatc cgcgcccgat gatcaactcc atttaccatc cccagaataa tttttgattg
     9781 gtgcgagcgc agcaggaacg atcgttactt taccgccgcg gtttcagcca ggggcgcgga
     9841 cgcgccaaga ttaaccaact gcgctgcggc cggcactcca tcctcggcgc aaaggccgat
     9901 gttgggcatt gcgtcctcga ttttcctgaa atggtctttc cacagcgggg cctggaaccg
     9961 tgccatccgt tccacttgcg cgcggcggcg gggattttgc agatccgcat agtccatgat
    10021 cgtgcgagcc cagccgcggc ctgcaagttg atcgacgtat tcgggaacat ctcccgcaat
    10081 ttcgcgcaac ggcggcagat cggaacagat gaccggcgtc ttcagggcca gcgcctcggc
    10141 caccggcagg ccgtagcctt cgacgtgcga gggcagcagc aaggccctgg cattggcgat
    10201 cagttcgatc agcaactcgt cgcagagctg attgcactcg atgacgtgac tgcggaggtt
    10261 ctcgccgcga tcgagcaggt cgaggacgtt ctcgttttcc cagccgcggc gaccgacgat
    10321 gaccagcttg ggcgccgccg ggccgtgctg cacgaccagc cgctgccaga cctgaagcag
    10381 caaaatgtgg ttcttgcgcg gctcgatggt gccgacgaaa acgaaatacg gtgcaaggtc
    10441 ggccttgggt tgcgtccgcg tgaaatgcgg actgatgccc aggggcgcga cgacggttct
    10501 gggcaccttg agtccgtggt cggcagcaaa ccggctcagg ctctcggcgg tgaattgaga
    10561 attggtgatg acggcgctgg cacagcgcgt caggtcttcg acgtatttcc gatgccgcgc
    10621 agccatcttc gggcgcgcgt actcggggtg cgtcaccggg atcaggtcgt gcagcatgaa
    10681 aatcccggaa acgtttgggc tttccttcag ccatcgctgg atggacggtg ccggcacgct
    10741 ggagcccgat acgcagatgt
//
LOCUS       DASZZC010000106         6231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_73296,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000106 DASZZC010000000
VERSION     DASZZC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73296"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2758)
                     /locus_tag="VHE37_02580"
     CDS             complement(<1..2758)
                     /locus_tag="VHE37_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34443.1"
                     /translation="MNLVLSRACRAVLALLLLGVTASAGANDPTPAAGTLSPDSRALA
                     FTAGPFPLSNESYSLPTGGMCSPQQVFRCDEYTLTLSLPAGYADAHPDDFVRITTAWP
                     DASEDFDIYILDPADSSGADLGNGQSTTGKDPEVAEIALKDIKDKTTLIVRIVPYNTK
                     GGTTATTIELITPVPPVPGPPPLAPSGEAPRFYNYAAPSAMGNGGEPSIGYNPMTKRA
                     MYFATGNGTTVPTGGSILQVTFPENLAAPLPEACDASWVDVTPPEMAVFDVDAILFTD
                     QSTGRTFASELLSVGPALAGLPGGLEGFNSLFAYTDDDGQTWVPGGLGPPQISVPPPV
                     IGTTVVDGLDHQTVGSGPYAAGAKPAIATSDIAVYYCSQGYVEAFCTRSDDGGLTFPS
                     LGSVIYTESNDGCGGLHGHVRVAPDGTVYVPNRNCNGKAAVAVSEDSGVSWTVHPDPL
                     SSSSDNDPQMALATDGTGYFCYVGSDGHPHVTVTHDKGVNWSDDYDVGYYAGVQSAVF
                     PQAIAGDPDRAACFFIGTSQAGNYQSMDFTGIWYPYVATTYDGGKTWHTVNISPNDPV
                     QREGGIWLRGGSETNRNLLDFNEITLDEKGRVMPIYADGCVGNCAQEGPNSFSAHAVI
                     ARQSGGRSLFAAMDSPPKAPLAACLAGQRDQHASHLNWRVPDNQGADISSYRIFRGSS
                     TDTQTLLTQVGADKPSFDDTTADPSVTNYSYKITAVNSVGESVYSNLITLPVTTLPVI
                     GTPCTLPGVAVITDPDGDNTSGPASTDLQSLSIAEPGDMDGKLVFTIKVASMTATPPP
                     GFRWVAYFVPPAAIPNGDENYYVAMVTDAGTPTFEYGTSVAVSPDPTGTAPAFRTYHR
                     IGDLDASSGFSTDGTIVLVFDKSNIPELVPGAALTGITASTRSTAPGPAGGQGLSLDT
                     SDTAG"
     gene            3043..3116
                     /locus_tag="VHE37_02585"
     tRNA            3043..3116
                     /locus_tag="VHE37_02585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3075..3077,aa:Gly,seq:ccc)
     gene            3137..3925
                     /locus_tag="VHE37_02590"
     CDS             3137..3925
                     /locus_tag="VHE37_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVT34444.1"
                     /translation="MTDISDPLVIAGKGYSSRLLVGTGKYRDFAQTRAAVEASGAQIV
                     TVAIRRSNIGQNKGEPNLLEVLPPSRYTILPNTAGCYTADDAVRTCRLARELLDGHAL
                     VKLEVLGDAKTLYPDVPATLKAAEALVEDGFQVMVYTSDDPIVCKRLEELGCVAVMPL
                     AAPIGSGLGIQNRYNILTIVENAKVPILVDAGVGTASDAAIAMELGCHGVLMNTAIAE
                     AKNPVLMAGAMKKAIEAGREAFLAGRMPRRRYASASSPESGLPF"
     gene            3935..4549
                     /locus_tag="VHE37_02595"
     CDS             3935..4549
                     /locus_tag="VHE37_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HVT34445.1"
                     /translation="MYTPGHFTGDSADALALMRAYPFATLITSAADGTHVTHLPLLVD
                     ESPALTLRAHMARANPHWRAFAQGATLAVFHGPHAYISPSWYVQPDREVPTWNYAAVH
                     AHGTPALIEDRGDKLALIDASVATFEAGNTPPWTRKVEGERLEMMLGAIVAFEMKVGH
                     LEAKFKMNQNRTADDRAKVIAQLRAAAHPDLQAMAAWMQAHEGS"
     gene            4536..5228
                     /gene="trmB"
                     /locus_tag="VHE37_02600"
     CDS             4536..5228
                     /gene="trmB"
                     /locus_tag="VHE37_02600"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HVT34446.1"
                     /translation="MKEAEPAHARRVRSFVRREGRFTDAQKSALERLWPRYGIDDGAG
                     VLDLRALFGRAAPVTLEIGFGNGDHLLARAGAEPQHDFIGVEVHRPGAGRLLLNAEKA
                     GIGNLRVSCTDAVDLLGQRIADGALAEVVIYFPDPWHKKRHHKRRLVQPEFAQLLARK
                     LARGGRLLLATDWADYAQHMLAVLNASPEFSNQSMDGSYVPRHATRPKTRFELRGEKL
                     GHAVFDLEYRRR"
     gene            5380..>6231
                     /locus_tag="VHE37_02605"
     CDS             5380..>6231
                     /locus_tag="VHE37_02605"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HVT34447.1"
                     /translation="MSAAHPIIGITGSSGAGTSTAVRVFARIFEQLEVKAAMIEGDAF
                     HAVDREQNKAAIARSHIRGENFSLFGPAGNLLGRLEQLLANYGARGQGVTRHYLHSEE
                     DALAHGHAVGTFTPWEKLPAGTDMLFYEGLHGGYVDAEIDIARHMDLLIGVVPVINLE
                     WIQKLKRDTGERGARAEDVTQTILRRMPDYVNHIVPQFSRTDVNFQRVPLVDTSNPFV
                     VRDIPGPQESLVVVHFNEQSRVGFDAKTFIERIDGAFQSHERTLVVPGPAMEAAMELV
                     ARPAIEQL"
BASE COUNT         1046 a         2011 c         2159 g         1015 t
ORIGIN      
        1 agcccgcggt gtcggaggtg tcgagagaaa gcccctggcc gccggccggg ccgggagcag
       61 tggagcgggt gctcgcggtg atgccggtca gcgcagcgcc gggcaccagt tcgggaatgt
      121 tcgacttgtc gaacaccagc acgatggtgc cgtcggtgga gaacccgctc gatgcatcga
      181 gatcgccaat cctgtgatag gtgcggaagg ccggcgccgt gccggtggga tcgggtgaga
      241 ccgcgacgct ggtgccgtac tcaaacgtgg gcgtgcccgc gtcggtgacc atggccacgt
      301 agtagttctc gtcaccgttg gggattgccg ctggcggcac gaaatacgcc acccagcgga
      361 agcctggcgg cggcgtggcg gtcatcgacg ccaccttgat cgtgaacacc agcttgccgt
      421 ccatgtcgcc gggctcggcg atcgacagcg actgcaggtc ggtgctcgcc gggccactgg
      481 tgttgtcgcc gtcagggtcg gtgatcaccg ccacgccggg cagcgtgcac ggcgtgccga
      541 tcaccggcag cgtggtcacc ggcagcgtaa tcaggttgct gtacacgctt tcgcccaccg
      601 agttcaccgc ggtgatcttg tagctgtagt tggtcaccga gggatcggcc gtggtgtcgt
      661 cgaagctggg cttgtcggcg cccacctggg tcagcagggt ctgcgtgtcg gtgctgctgc
      721 cgcggaagat cctgtacgag ctgatgtccg cgccctggtt gtccggcacg cgccagttca
      781 ggtgcgacgc gtgctggtcg cgctggccgg ccaggcacgc cgccagcggt gccttgggcg
      841 gcgagtccat cgccgcgaac agcgagcggc cgccgctctg gcgcgcgatc accgcgtgcg
      901 cggagaagct gttggggcct tcctgcgcgc agttgcccac gcagccgtcg gcgtagatcg
      961 gcatcacgcg gcctttctcg tccagcgtga tctcgttgaa gtcgagcagg ttgcggttgg
     1021 tctcgctgcc gccgcgcagc cagatgccgc cttcgcgctg caccgggtca ttgggcgaga
     1081 tgttcaccgt gtgccaggtc ttgccgccgt cgtaggtggt ggccacgtac gggtaccaga
     1141 tgccggtgaa gtccatcgac tggtaattgc cagcctggct ggtgccgatg aagaagcagg
     1201 ccgcgcggtc gggatcaccg gcaatcgcct gcgggaacac cgcgctctgc acgccggcgt
     1261 agtagccgac gtcgtaatcg tcgctccagt tgacgccctt gtcgtgggtg acggtgacat
     1321 gcgggtggcc gtcgctgccc acgtagcaga aatagccggt gccatcagtg gccagcgcca
     1381 tctgcggatc gttgtcgctg ctgctggaga gcggatcggg atgcacggtc cagctcacgc
     1441 cgctgtcctc ggacaccgcg acggcagcct tgccgttgca gttgcggttg ggcacgtaga
     1501 cggtgccgtc cggcgccacg cgcacgtggc cgtgcaggcc gccgcagccg tcattggatt
     1561 cggtgtagat caccgagccg agcgagggga aggtcaggcc gccgtcgtcg ctgcgcgtgc
     1621 agaacgcttc gacataaccc tgcgagcagt aatacaccgc gatgtccgag gtggcgatgg
     1681 ccggcttggc gcccgcggcg tacggaccgc tgccgacggt ctggtgatcg agcccgtcga
     1741 ccacggtggt gccgatcacc ggcggcggca ccgaaatctg cggcgggccc agcccgcccg
     1801 gcacccaggt ctggccgtcg tcatcggtat aggcaaacag gctgttgaag ccttcgagcc
     1861 cgccgggcag tccggccagc gcggggccga ccgagagcag ttccgaggcg aaagtgcggc
     1921 cggtcgactg gtcggtgaac aggatggcat cgacatcgaa taccgccatc tccggcggcg
     1981 tcacgtccac ccagctggca tcgcaggctt cgggcagcgg cgcggccagg ttttccggga
     2041 aggtgacctg caggatcgat ccgccggtgg gcacggtggt gccgttgccg gtggcgaagt
     2101 acatggcgcg cttggtcatc gggttgtagc cgatcgacgg ttcgccgccg ttgcccatgg
     2161 cagacggcgc cgcgtagttg tagaagcgcg gtgcttcgcc gctcggcgcc agcggcggcg
     2221 gacccggcac cggaggcacc ggcgtaatca gctcgatggt ggtggcggtg gtgccgccct
     2281 tggtgttgta cggcacgatg cgcacgatca gcgtggtctt gtccttgatg tccttcagcg
     2341 caatctccgc cacctcgggg tccttgccgg tggtcgactg gccattgcca agatcagcgc
     2401 cgctgctgtc ggcgggatca aggatgtaga tgtcgaagtc ctcgctcgca tccggccagg
     2461 cggtggtgat gcgcacgaag tcgtccgggt gcgcatcggc atagcccgcc ggcagcgaca
     2521 gcgtgagcgt gtattcgtca cagcggaaca cctgctgcgg cgaacacatg ccgccggtgg
     2581 gcagtgaata cgattcgttg ctgagcggaa acggcccggc ggtgaacgcc agggcgcggc
     2641 tgtcgggcga cagtgtgccg gcggcgggcg tgggatcgtt ggcaccggcc gacgcggtca
     2701 cgcccagcag caacaacgca agaacggcgc ggcatgcgcg cgacaagacg agattcatcc
     2761 ccacactccg gcagacgcgg tcaccgcgcc gcggctatcg ccgctgaaag aaagaaaaga
     2821 gatccagggc cgtcacggcc gagcggttcc agtggaacat catgcagcgc cttctcccag
     2881 gaaggccggc atcgtacacc cgcgtttgcc tgcggggcac gaggaaaaac cactaaagcg
     2941 ggcgcattcc gggagcaggc gaaaaaagtt aaccggctgt taaagcggcg ccggggctgc
     3001 cggtcgtacc gctggcgcgg cgcttggaga aaatgcgcgg acgcgggtgt agctcaatgg
     3061 tagagctgta gcttcccaag ctactgacga gggttcgatt cccttcaccc gctccacttt
     3121 tgccggcgcc gcatccatga ctgacatttc cgatccgctg gtcatcgcgg gcaagggcta
     3181 ttcctcgcgc ctgctggtgg gcaccggcaa gtaccgggac tttgcgcaga cgcgagcggc
     3241 ggtggaagcc agcggcgcgc agatcgtcac cgtcgccatc cgccgcagca acatcggcca
     3301 gaacaagggc gagccgaacc tgctcgaggt gctgccgccg tcgcgctaca ccatcctgcc
     3361 caacacggcc ggctgctata ccgcggacga tgccgtgcgc acctgccgcc tggcgcgcga
     3421 actgctcgac ggccacgcgc tggtcaagct cgaagtgctg ggcgatgcca agaccctgta
     3481 ccccgacgtg ccggccacgc tcaaggctgc cgaggcgctg gtggaggacg gcttccaggt
     3541 catggtctac accagcgacg atcccatcgt gtgcaagcgg ctggaggaac tcggctgcgt
     3601 ggcggtgatg ccgctggccg cgccgatcgg ttcggggctg ggcatccaga accgctacaa
     3661 catcctcacc atcgtcgaga acgccaaggt gccgatcctg gtcgatgccg gcgtcggcac
     3721 cgcgtccgac gcggcgatcg cgatggagct gggctgccac ggcgtgctga tgaacacggc
     3781 aatcgcggaa gcgaagaacc cggtgctgat ggcgggcgcg atgaagaagg cgatcgaggc
     3841 cgggcgcgag gcgtttctcg ccgggcgcat gccgcggcga cgctacgcga gcgcctcctc
     3901 gccggaatcg ggactgccgt tctgagtcga ttcgatgtac acgcccggac atttcaccgg
     3961 cgatagcgcc gacgcgcttg cgctgatgcg cgcgtacccg ttcgccacgc tgattaccag
     4021 cgcggcggac ggcacgcatg tcacgcacct gccgctgctg gtggacgagt cgccggcgct
     4081 gacgctgcgc gcgcacatgg cgcgcgccaa tccgcactgg cgcgcgttcg cccagggcgc
     4141 cacgctcgcg gtgtttcacg gcccgcatgc atacatctcg ccctcgtggt acgtgcagcc
     4201 ggaccgcgaa gtgcccacct ggaactacgc ggcggtgcac gcgcacggca cgcccgcgct
     4261 gatcgaggat cgcggcgaca agctcgcgtt gatcgatgcc agcgtcgcca cgttcgaggc
     4321 cggcaacacg ccgccgtgga cgcgcaaggt ggaaggcgag cggctggaga tgatgctcgg
     4381 cgcgatcgtg gcgttcgaga tgaaagtcgg gcacctcgaa gccaagttca agatgaacca
     4441 gaaccgcacg gcagacgacc gcgcaaaggt gatcgcgcaa ctgcgcgccg ccgcgcatcc
     4501 cgacctgcag gcgatggccg catggatgca ggcgcatgaa ggaagctgag ccggcgcacg
     4561 cgcgccgcgt gcgcagtttc gtgcggcgcg agggccgctt caccgacgcg cagaaatccg
     4621 cgctcgagcg cctgtggccg cgctacggca tcgatgacgg cgcaggcgtc ctcgacctgc
     4681 gtgcgctgtt cggccgcgcg gcgccggtga cgctggagat cggctttggc aacggcgatc
     4741 acctgctggc gcgcgccggc gcagaaccgc agcatgattt catcggcgtg gaagtgcacc
     4801 ggcctggcgc cggccgcctc ctgctcaacg cggagaaagc cggcatcggc aacctgcgcg
     4861 tgagctgcac ggatgcggtc gacctgctgg gccagcgcat cgccgacggc gcgctggccg
     4921 aggtggtcat ttactttcca gatccctggc acaagaagcg gcaccacaag cgacggctgg
     4981 tgcagccgga attcgctcag ctgctcgcac gcaaactcgc gcgcggcggc cgcctgctgc
     5041 tggcgacgga ctgggccgac tacgcgcagc acatgctggc ggtgctcaac gcatcgccgg
     5101 aattttccaa ccagtcaatg gacggcagct acgtgccgcg ccatgcgacg cggccgaaga
     5161 cgcgcttcga gttgcgcggc gagaagctcg gccacgccgt gttcgatctc gaataccgcc
     5221 gccgctgatc gcgcgcgagc tttttttgtt ccaccgcact gccgctttgc ttgttattaa
     5281 ttatttatca ttttataaat aaaacaatat ttaataacac gcaaaatggc ggtgcggtgg
     5341 agtcaaaaca ccgagctttg caggcaagat aagaacatca tgtcggccgc acatcccatc
     5401 atcggcatca ccggctcatc cggcgcgggc accagcaccg cggtgcgcgt gttcgcgcgc
     5461 atctttgagc agctggaggt gaaggcggcg atgatcgagg gcgatgcctt ccacgccgta
     5521 gaccgcgagc agaacaaggc cgccatcgca cgctcgcaca tccgcggcga aaatttctcg
     5581 ctgttcggcc cggccggcaa cctgctcggc cggctggagc agctgcttgc gaactacggc
     5641 gcgcgcgggc agggtgtcac gcgccactac ctgcacagcg aggaagacgc gctcgcgcac
     5701 ggccacgcgg tgggcacctt cacgccgtgg gagaagctgc cggcgggcac cgacatgctg
     5761 ttttacgaag gcctgcacgg cggctacgtc gatgccgaga tcgacatcgc gcggcacatg
     5821 gacctcttga tcggcgtggt gccggtgatc aacctggagt ggatccagaa actcaagcgc
     5881 gacaccggcg agcgcggggc gcgcgccgag gacgtgacgc agaccatcct gcgccgcatg
     5941 cccgattacg tgaaccacat cgtgccgcag ttctcgcgca ccgacgtgaa tttccagcgc
     6001 gtgccgctgg tcgataccag caaccccttc gtggtgcgcg acattcccgg tccgcaggag
     6061 agcctggtgg tggtgcattt caacgagcag tcgcgtgtcg gcttcgacgc caagacgttc
     6121 atcgagcgca tcgatggcgc attccagtcg catgagcgca cgctggtggt gccgggccct
     6181 gcgatggaag cggcgatgga gctggtcgcg cgcccggcca tcgaacagct g
//
LOCUS       DASZZC010000107         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_199893,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000107 DASZZC010000000
VERSION     DASZZC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199893"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /gene="rsmB"
                     /locus_tag="VHE37_02610"
     CDS             <1..1306
                     /gene="rsmB"
                     /locus_tag="VHE37_02610"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HVT34448.1"
                     /translation="PMKPEANPRVQAARAVARVMAGASLDDALARIDARAISPQDLSL
                     AKAIAYGVVREHRLLGDLATRLLERPIKNAPEAQALLLCGLYQLRSMRIAEHAAVGET
                     VEAVALIGKPSLRGLVNAVLRRYQREKEGLEAVLPRQPAAQYSYAEWMAERIAQDWGT
                     QAPAVLAAGNAQAPLTLRVNRRKITREAYQLKLAVTGIPAHEVAHAPDAVALEAALPV
                     EDVPGFDNGLVSVQDASAQLAAELLALQPGQRVLDACAAPGGKTAHLLEHADVQVLAL
                     DRDAERTRRIGQNLDRLDLQAEVVTADAADPAAWWDGKPFERILLDAPCSGTGVIRRH
                     PDIKWLRRPSDIPRMAAQQQALLDALWPALAPGGLLLYAVCSIFSAESESVLKNFLVG
                     HADARRLVIDTPWGEACKVGRRIAPGGDFDGFYYARLQKPAR"
     gene            1342..>1916
                     /locus_tag="VHE37_02615"
     CDS             1342..>1916
                     /locus_tag="VHE37_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HVT34449.1"
                     /translation="MAATEIQNMALYCDFENVALGVSELGGDLRFDVRKVLDRLLLKG
                     DIVVKKAYCDWVRYEGFKKAMHEAAFELIDIPHTRQAGKNSADIRMVVDALDLCYTKA
                     HVDCFVIISGDSDFSPLVSKLRENNKVVIGVGVRGSTSPLLRDNCDEFIFYDDLVKEE
                     QKKEQKRHEQHAQKEKARKDALAKQQKAQQSA"
BASE COUNT          336 a          633 c          664 g          283 t
ORIGIN      
        1 gccgatgaag cctgaagcca atccgcgcgt gcaggcggcg cgcgcggtgg cgcgcgtgat
       61 ggccggcgcc agcctggacg acgcgctggc ccgcatcgac gcgcgcgcca tatcgccgca
      121 ggacctctcg ctggccaagg cgatcgccta cggcgtggtg cgcgagcatc gcctgctggg
      181 cgatctcgcc acgcgcctgc tcgaacgtcc gatcaagaac gcgccggagg cacaggcgct
      241 gctgctgtgc ggcctgtacc agctgcgctc gatgcgcatt gccgagcacg ccgcggtggg
      301 cgagacggtg gaggccgtgg cgctgatcgg caagcccagc ctgcgcggcc tggtcaacgc
      361 cgtgctgcgg cgctaccagc gcgagaagga aggcctggag gcggtgctgc cgcgccagcc
      421 ggccgcgcag tactcttatg cggagtggat ggcagagcgc atcgcgcagg attggggcac
      481 gcaggcaccg gcggtgctcg ccgccggcaa tgcgcaggcg ccgctgacct tgcgcgtgaa
      541 ccgccgcaag atcacgcgcg aggcctacca gctcaagctg gcggtgaccg gcatcccggc
      601 gcacgaggtg gcgcacgcgc cagacgcagt cgcgctggag gccgcgctgc cggtggagga
      661 tgtccccggc ttcgacaacg gcctggtatc ggtgcaggac gcttctgcgc aactcgctgc
      721 agaactgctc gcgctgcagc cgggccagcg cgtgctcgac gcctgcgccg cgcccggcgg
      781 caagaccgcg cacctgctcg aacacgcgga cgtgcaggtg ctggcgctcg accgcgacgc
      841 cgagcgcacg cggcgcatcg gccagaatct cgaccgcctc gacctgcagg ccgaagtggt
      901 caccgccgac gcggccgatc ccgccgcgtg gtgggacggc aagccgttcg agcgcatcct
      961 gctcgacgca ccgtgctcgg gcaccggcgt gatccggcgc catcccgaca tcaagtggct
     1021 gcgccggccg tcggacatcc cgcgcatggc cgcgcagcag caggcgctgc tcgatgcgct
     1081 gtggccggca ctcgcgcccg gcggactgct gctgtacgcg gtgtgctcga tcttttccgc
     1141 ggaaagcgaa agcgtgctga agaatttcct ggtcggccat gccgacgcgc ggcgcctggt
     1201 gatcgacacg ccgtggggcg aggcatgcaa ggtgggcaga cgcatcgcgc ccggcggcga
     1261 tttcgatggc ttctactacg cgcgcctgca gaagccggcg cgctgactgc gctaatctgc
     1321 gcgctccaat cgagagcgat catggccgca accgaaatcc agaacatggc gctttactgc
     1381 gacttcgaga atgttgcgct gggggtcagc gagcttggcg gcgatctgcg tttcgacgtg
     1441 cgcaaggtgc tcgatcgcct gctgctcaag ggcgatatcg tggtgaagaa ggcctattgc
     1501 gactgggtcc gctacgaagg attcaagaag gcgatgcacg aagcagcgtt cgaactgatc
     1561 gatatcccgc acacgcgcca ggccggcaag aactccgccg acatccgcat ggtggtcgat
     1621 gcgctcgacc tctgttacac caaggcccac gtcgactgct tcgtcatcat ctccggcgat
     1681 tccgacttct cgccactggt gtccaagctg cgcgagaaca acaaggtggt gatcggtgtg
     1741 ggcgtgcgtg gctccacctc gccgctgtta cgcgacaact gcgacgagtt catcttttac
     1801 gatgacctgg tcaaggaaga acagaagaag gagcagaaac ggcacgagca gcacgcgcaa
     1861 aaggagaagg ccaggaagga tgcattggcc aagcagcaga aggcccagca aagcgc
//
LOCUS       DASZZC010000108         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_73694,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000108 DASZZC010000000
VERSION     DASZZC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73694"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VHE37_02620"
     CDS             complement(<1..748)
                     /locus_tag="VHE37_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT34450.1"
                     /translation="MLSFAAKRLLASVPTLLVLITLAFFMMHAAPGGPFDRERALPAE
                     IEAAMKSAYHLDEPVMQQYLRYMGGLLHGDLGPSFQYPGFSVSELIASGFPVSLQLGL
                     WSMLLALPLGCAAGMLAAVRQNRAADHALMAVALAGMSIPTYVIAPLLVLLFAVSLGW
                     LPAGGWEHGRYADMVMPVLALAAPQIAYIARLMRGATIEVLRSNFIRTARAKGLPAHR
                     VLLRHAIKPALIPVLSYLGPAAAGIITGSVV"
     gene            complement(748..>1540)
                     /locus_tag="VHE37_02625"
     CDS             complement(748..>1540)
                     /locus_tag="VHE37_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT34451.1"
                     /translation="IRRTFGGELRIAPYAGNYYYGYNLTQPPFKDNPKLREALALAID
                     RELIVSKVMNGVALPAYGWVPPGMWNYTPQQPAWAAWTREQRIARARQLYAEAGYDAQ
                     HPAQVEIRYNTHEDHKRIAVVIAAMWKQYLGVQATLVNEEFKVFLNNRKLKRATQAFR
                     ASWVADYDDASAFSDILRSTHGENDSGYVSAAYDALLAQAAVEPDVARRRELLQQAER
                     QVLTDWPVLPIYYYVSKHLVKSYVQGWEDNILDYHYSKDLRIAPH"
BASE COUNT          257 a          507 c          527 g          249 t
ORIGIN      
        1 tcaccaccga gccggtgatg atgcccgccg ccgccgggcc gagatacgac agcaccggga
       61 tcaacgccgg cttgattgcg tggcgcagca gcacgcgatg cgccggcagg cctttcgcgc
      121 gtgcggtgcg gatgaagttg gagcgcagca cttcgatggt ggctccgcgc atcagccgcg
      181 cgatgtaggc gatctgcggc gcggcgagcg ccagcaccgg catcaccatg tcggcgtagc
      241 gcccgtgctc ccaaccaccc gccggcagcc agccgaggct cactgcaaac agcaggacca
      301 gcagcggcgc gatgacgtag gtgggaatcg acatgccggc cagcgcgacg gccatcagcg
      361 catgatccgc ggcgcggttc tggcgcaccg cggccagcat gccggccgcg cagcccagcg
      421 gcagcgccag cagcatcgac cacagcccga gctgcagcga aaccgggaag ccgctggcga
      481 tgagttcgct gacggaaaaa cccgggtact ggaacgaagg cccaaggtca ccgtgcagca
      541 gtccgcccat gtagcgcagg tattgctgca tgactggctc gtcgaggtga taggcgcttt
      601 tcatcgccgc ctcgatttcc gccggcagcg cgcgctcgcg gtcgaacgga ccgccgggcg
      661 cggcatgcat catgaagaag gcgagcgtga tcagcaccag aagagtgggc acgctggcca
      721 gcaggcgctt ggcggcgaac gagagcatca gtgcggcgcg atgcgcagat ccttgctgta
      781 gtgataatcg aggatgttgt cctcccagcc ctgcacgtag ctcttcacca ggtgcttgga
      841 gacgtaatag tagatcggca gcaccggcca gtcggtgagc acctggcgct cggcctgctg
      901 cagcagctcg cgccggcgcg ccacgtcggg ctcgaccgcg gcctgcgcca gcagcgcatc
      961 gtaggccgcg ctcacgtaac ccgaatcgtt ctcgccgtgc gtcgaacgca ggatgtcgga
     1021 gaaggcgctg gcgtcatcgt agtcggccac ccatgacgcg cggaacgcct gcgtggcgcg
     1081 cttgagcttg cggttgttga ggaagacctt gaactcctcg ttgaccagcg tggcctgcac
     1141 gcccaggtac tgcttccaca tggcggcgat gaccaccgcg atgcgcttgt gatcctcgtg
     1201 cgtgttgtag cggatttcca cctgcgccgg atgctgcgcg tcgtagcccg cttctgcgta
     1261 cagctggcgt gcgcgtgcga tgcgctgctc gcgcgtccat gcggcccatg ccggctgttg
     1321 cggcgtgtag ttccacatgc ccggcggcac ccagccgtag gcaggcagcg cgacgccgtt
     1381 catcaccttg ctcacgatca gctcgcggtc gatggccagc gccagcgcct cgcgcagttt
     1441 cgggttgtcc ttgaacggcg gctgcgtcag gttgtagccg tagtagtagt tgccggcata
     1501 gggcgcgatg cgcagctcgc cgccgaaggt gcggcggatc
//
LOCUS       DASZZC010000109         4867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_391748,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000109 DASZZC010000000
VERSION     DASZZC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391748"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..989
                     /locus_tag="VHE37_02630"
     CDS             114..989
                     /locus_tag="VHE37_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HVT34452.1"
                     /translation="MQMREFVLSRNDFRRVRAMIHARAGIALSDAKKDMVFSRLVRRL
                     RALQLDSFNDYLDQLEQHPAWEEWQEFTNALTTNLTSFFREAHHFEMLRQQFLASKSR
                     PFRVWCAASSTGEEPYSLAITLAEAAHSLAPPVQIIASDIDTRVLETARAGVYPLERL
                     EKLSRERIKRYFQRGTGRNEGYCRVVEPLRKLIDFREINLLDGRWDFDAPFDAIFCRN
                     VMIYFDKPTQRRVLEKFLPLLRPDGRLYAGHSESFLHSSHLFHSCGRTVYAPVTAQAP
                     ARAAAHAAVRVKEPA"
     gene            986..1600
                     /gene="cheD"
                     /locus_tag="VHE37_02635"
     CDS             986..1600
                     /gene="cheD"
                     /locus_tag="VHE37_02635"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HVT34453.1"
                     /translation="MSMPATAWEMQAESTGLTYFDRQFNITAQKVLPGECSVTRENIM
                     LVTVLGSCVAACIRDPLAGVGGMNHFMLPESELGGSNSARYGSYAMEILINQLLKNGA
                     RRERMQAKVFGGGNVLRGFTVNQVGTRNADFVRGYLAAEGIAIAAEDLGDVHPRKIFY
                     FPRDGRVMVKKLNAVQSTVDLSSERAYQQRLAAERIGGDVELFE"
     gene            1597..2628
                     /locus_tag="VHE37_02640"
     CDS             1597..2628
                     /locus_tag="VHE37_02640"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HVT34454.1"
                     /translation="MKTRVLVVDDSALVRQVLTELLSADPEIEVVGTASDPLVARDKI
                     KQLHPDVLTLDVEMPRMDGLTFLENLMRLHPLPVLMVSSLTERGAEVTLQALELCAVD
                     FVTKPKLDVANGLAGYAQELRDKVRMAARSRPRGAAPARERGRPISRPFRTTERLIAI
                     GASTGGTEAIKHVLERMPADAPAIVITQHIPAAFSGPFAQRMDRCSAMSVCEASDGQQ
                     IVPGHAYIAPGGRHLVVARDGARYVCRITDTAPENRHRPSVDVLFRSVAQHAAANAVG
                     AILTGMGDDGARGLLHMRERGAHTLAQDEKSSVVWGMPGAAVKMQAVEQILPLDDIAE
                     RLLMLSAAA"
     gene            2683..3801
                     /locus_tag="VHE37_02645"
     CDS             2683..3801
                     /locus_tag="VHE37_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVT34455.1"
                     /translation="MRGQDMKIFAVPAALSAAQLLLLPFNTPLALVALLGLAAAAGWA
                     WTLMGLAQQVREQHGATVALHAKLAELRAGVAKETGGASEEIARVRALVQEAIAKLSA
                     SFDQVNRESGQQASAVTQILNRTGVDAGEGMSVRKFAHQASRLMEDQVDVLADVSKQS
                     AASVGNIDEMVKHLDAIFDLLGDVKSIADQTNLLALNAAIEAARAGEAGRGFAVVAEE
                     VRNLSERSTSFNEQIRKLVFSSKDAVARVREAVGRMATRDMDASVKSREQVRGLVGQV
                     EDIDRAFAEGMQKVSGTGQKISASVSEAVRCLQFEDIATQALAAAQRHIGNLGAIGSD
                     AGVAIGLPPAPPPIPDWRAPQHKPVSQVSMQAGEVELF"
     gene            complement(3918..>4867)
                     /locus_tag="VHE37_02650"
     CDS             complement(3918..>4867)
                     /locus_tag="VHE37_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT34456.1"
                     /translation="LSVVTPGCDWARMVEEAGQACENAKQRGFNQVSRNRRRAASHPA
                     TSPHSSDLIANFRKYALQERLALHPQPIMDISGPEPRLAKAEFLLRYIDGERATPLPA
                     HTIETLEHFGLAAELDQLASRQLLEWLSNNPATLARLESVSLNLSARSIIDGHFINRL
                     YSEVKNAHLPEGKLCFEITETAAIENLEVASEVIALFQDIGCKFSLDDFGSGLCSFGY
                     LHTLHVDEVKIDGRFTRDITRSFVSLEIVRAIQQVAKATGKKTVAEFVDAPEKLAVLQ
                     DIGVDYGQGWLFSPCITPEMLKQMLLAPGAIPATAATTF"
BASE COUNT          812 a         1578 c         1716 g          761 t
ORIGIN      
        1 ggcgccggcg aagccagccc cagcggccgc caagtcggag gagcagtgga ccgagttctg
       61 agtttcaacc gctgaagaga gcgcggctgc cggcaccccc ggcagccgcg atcatgcaga
      121 tgcgcgaatt cgtcctcagc cgcaatgact tcagacgcgt ccgcgcgatg atccacgcgc
      181 gcgccggcat cgccctgtcc gacgccaaga aggacatggt gttcagccgg ctggtgcggc
      241 gcctgcgcgc gctgcagctg gattcgttca atgactacct cgaccagctg gagcagcatc
      301 cggcctggga ggagtggcag gaattcacca acgcgctgac caccaacctc acctcgttct
      361 tccgcgaggc gcatcacttc gaaatgctgc gccagcagtt cctggcatcg aagtcgcgcc
      421 cgttccgcgt ctggtgcgcg gcgtcttcga ccggcgagga gccttattcg ctggccatca
      481 cgctggccga ggcggcccac agcctggcgc cgccggtgca gatcattgcc agcgacatcg
      541 atacgcgcgt gctggagacc gcgcgcgccg gcgtctaccc gctggagcgg ctggaaaagc
      601 tgagccgcga gcggatcaag cgttacttcc agcgcggcac cggccgcaac gagggctact
      661 gccgcgtggt ggaaccgctg cgcaagctga tcgacttccg cgagatcaac ctgctggacg
      721 ggcgctggga cttcgatgcg ccgttcgacg ccatcttctg ccgcaacgtg atgatctatt
      781 tcgacaagcc cacgcagcgg cgcgtgctgg agaaattcct gccgctgctc aggcccgacg
      841 ggcgcctata tgccgggcac tcggagagct tcctgcattc ctcgcacctg ttccattcct
      901 gcgggcgcac ggtgtatgcg ccggtgacgg cgcaggcgcc ggcgcgcgcc gcggcgcatg
      961 cggccgtgcg ggtgaaggag ccggcatgag catgccggcc acggcgtggg agatgcaggc
     1021 cgagagcacc ggcctcacct acttcgaccg ccagttcaac atcaccgcgc agaaggtgct
     1081 gcccggcgaa tgcagcgtga cgcgcgagaa catcatgctg gtcaccgtgc tcggttcctg
     1141 cgtggcggcc tgcatccgcg acccgctggc cggagtcggc ggcatgaatc acttcatgct
     1201 gccggagagc gagctgggtg gcagcaactc ggcgcgctac ggcagttacg ccatggaaat
     1261 cctgatcaac cagctgctca agaatggcgc gcggcgcgaa cgcatgcagg ccaaggtgtt
     1321 cggcggcggc aacgtgctgc gcggtttcac cgtcaaccag gtgggcacgc gcaacgcgga
     1381 tttcgtgcgc ggctatctcg cggccgaggg cattgccatc gcggccgaag acctgggcga
     1441 cgtgcacccg cgcaagatct tctacttccc gcgtgatggc cgcgtcatgg tgaagaagct
     1501 caacgcggtg cagagcacgg tggacctgtc gagcgagcgc gcctaccagc agcggctggc
     1561 ggcggagcgc atcggcggcg acgtggagct gttcgaatga aaactcgcgt gctggtggtg
     1621 gacgactcgg cgctggtgcg gcaggtgctc accgagctgc tgtcggccga cccggagatc
     1681 gaagtggtgg gcaccgcctc cgacccgctg gtggcgcgcg acaagatcaa gcagctgcac
     1741 ccggacgtgc tgacgctcga tgtcgagatg ccgcgcatgg atggcctcac cttcctggaa
     1801 aacctgatgc gcctgcaccc gctgccagtg ctgatggtgt cgtcgctgac cgagcgcggg
     1861 gccgaggtga cgctgcaggc gctggaactg tgtgcggtgg atttcgtcac caagcccaag
     1921 ctggacgtgg ccaacggcct ggccggctac gcgcaggaac tgcgcgacaa ggtgcggatg
     1981 gcggcgcgct cgcgtccgcg cggcgcggcg ccggcgcgcg aacgcggccg gccgatctcg
     2041 cggccgttcc gcaccaccga gcggctgatc gcgatcggcg cgtccaccgg cggcaccgag
     2101 gcgatcaagc acgtgctcga acgcatgccg gcggatgcgc cggccatcgt catcacccag
     2161 cacatacccg cagcgttcag cggcccgttc gcccagcgca tggaccgctg ctcggccatg
     2221 tcggtgtgcg aggccagcga cggccagcag atcgtgcccg gccatgccta catcgcgccg
     2281 ggcgggcggc acctggtggt ggcgcgcgac ggtgcccgct acgtctgccg catcaccgac
     2341 acggcgccgg aaaaccgcca tcggccgagc gtggatgtgc tgttccgctc ggtggcgcag
     2401 cacgccgcgg ccaacgcggt gggcgccatc ctcaccggca tgggcgacga cggcgcgcgc
     2461 ggcctgctgc acatgcgcga gcgcggcgcg cacacgctgg cgcaggacga gaagagcagc
     2521 gtggtctggg gcatgcccgg cgcggcggtg aagatgcagg cggtggaaca gatcctgcct
     2581 ctggacgaca ttgcggagcg gctgctgatg ctgtcggccg ccgcctaaag aaagggcgct
     2641 aagcgccgct acccctcaag agtcatttcg gcaggagccc gtatgcgtgg ccaggacatg
     2701 aagatcttcg cggtgccggc ggccctcagt gccgcgcagc tgttgctgct gccgttcaac
     2761 acgccgctgg cgctggtggc gctgctgggg ctggccgctg ccgcgggctg ggcctggacg
     2821 ctgatgggcc tggcgcagca ggtgcgcgag cagcacggcg ctaccgtcgc gctgcacgcg
     2881 aagctggccg aactgcgcgc cggcgtggcc aaggaaaccg gcggagcctc cgaggaaatc
     2941 gcgcgcgtgc gcgccctggt gcaggaagcc atcgccaagc tgtcggccag cttcgaccaa
     3001 gtgaaccgcg agtcaggcca gcaggccagc gcggtgacgc agatattgaa ccgcactggc
     3061 gtcgacgccg gcgagggcat gagcgtgcgc aagttcgcgc accaggccag ccggctgatg
     3121 gaggaccagg tggacgtgct ggccgatgtc agcaagcaga gcgcggcctc ggtgggcaac
     3181 atcgacgaga tggtcaagca tctcgacgcc atcttcgacc tgctgggcga cgtgaagtcg
     3241 atcgccgacc agaccaacct gctggcgctc aacgccgcca tcgaggcggc gcgcgcgggc
     3301 gaggccgggc gcggattcgc cgtcgtcgcc gaggaagtgc gcaacctgtc ggaacgctcg
     3361 accagcttca acgagcagat ccgcaagctg gtgttcagca gcaaggacgc ggtggcgcgc
     3421 gtgcgcgagg cggtgggccg catggccacc cgcgacatgg acgccagcgt caagtcgcgc
     3481 gagcaggtgc gcgggctggt cggccaggtg gaagacatcg accgcgcctt cgccgagggc
     3541 atgcagaaag tgtcgggcac cgggcagaag atctcggcgt cggtgtccga ggcggtgcgc
     3601 tgcctgcagt tcgaggacat tgccacgcag gcgctggccg cggcgcagcg gcatatcggc
     3661 aatctcggcg ccatcggttc ggatgcgggc gtggccatcg gcctgccgcc ggcgccgccg
     3721 ccgattcccg actggcgtgc cccgcagcac aagccggtgt cgcaggtatc gatgcaggcg
     3781 ggcgaggtgg agctgttctg aagctccacc gcaccctggt tttgcttgtt ataaaatatt
     3841 tattaaataa taatttatac aataataatt aacagccaat ggctgtgcgg tgaggccgaa
     3901 aaaaccgctc gcgcgattca gaaggtggtt gcggccgtgg ccggaatggc gcccggcgcg
     3961 agcagcatct gcttgagcat ttccggcgtg atgcagggcg agaacagcca gccctggccg
     4021 tagtcgacgc cgatatcctg cagcaccgcg agtttctcgg gcgcgtcgac gaattccgcc
     4081 acggttttct tgccggtggc cttggccacc tgctggatgg cgcgcacgat ctccagcgag
     4141 acgaagctgc gcgtgatgtc gcgggtgaag cggccgtcga tcttcacttc gtccacgtgc
     4201 agcgtgtgca ggtagccgaa cgagcacagg ccggaaccga agtcgtcgag cgagaacttg
     4261 cagccgatgt cctggaacag cgcgatgact tccgaggcca cttccaggtt ttcgatggcg
     4321 gccgtctcgg tgatctcgaa gcacagcttg ccttccggca ggtgcgcgtt cttcacctcg
     4381 ctgtacaggc ggttgatgaa gtggccgtcg atgatcgagc gcgccgacag gttgagcgac
     4441 accgattcca gccgcgccag cgtggccggg ttgttcgaca gccattcgag caactggcgc
     4501 gaggccagct ggtcgagctc ggcggccagg ccgaaatgct ccagcgtctc gatggtgtgg
     4561 gccggcagcg gcgtggcgcg ctcgccgtcg atgtagcgca gcaggaactc ggccttggcc
     4621 aggcgcggct ccggcccgct gatgtccatg atcggctgcg gatgcagcgc aagcctttcc
     4681 tgcagtgcgt acttgcggaa gttggcgatg agatcggagc tgtgcggcga ggtcgcaggg
     4741 tggctcgcgg cgcggcggcg gttgcgcgac acctggttga agccgcgctg cttggcgttc
     4801 tcgcaggcct ggccggcttc ttccaccatg cgcgcccagt cacagccggg cgtgaccacc
     4861 gacaggc
//
LOCUS       DASZZC010000110         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_971213,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000110 DASZZC010000000
VERSION     DASZZC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971213"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2260
                     /locus_tag="VHE37_02655"
     CDS             <1..>2260
                     /locus_tag="VHE37_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HVT34457.1"
                     /translation="LDDQRHNIRNARFTLERLDAGNTREGFELDGALRLPSVYGESLR
                     FEAAVRGHIGRPQQWRGGFRLRGDNLLPQMWLRGHVAPGTRVAAENIGLDLQGELAGG
                     ALSAVRLTLTSGTLAASRPRQDLQLQSLRAALSATRDAAGWLLRVQQLDIDGETQARG
                     ELRYTPSAQGYAVKVEAPLLRLDRIAPWLGFARTLPGWLQHAAGASGEIDDAVLDLRQ
                     DGDARHYAARASLRQLQFASSDGLPGVGGISGELSADENGGRFTLGGGGVALDLPATF
                     AAPVQLDQLAGEVSWQQVPEGWQISAPVLDWRLAGGAGRARVGLLLPAGERSPELDLG
                     ASFAFPDAVALKGLMPLHWSEGLRSWLDRGIVAARIPSARLAIHGALADFPFVDGKPG
                     EWKLDVSVAGATLAYAARWPQADNLRAQLHFRGNSLSIESDGADLGGVHARRINAEFG
                     DFRESHLAIDGQVSGEMVRYYSFLQNSPLRETLSGLLDNTRAAGPAEVSLHLDIPLRD
                     AQRTAASGLIRLDGVELYYGSLADPVSDIHGELAFDQDGVSGQDLTAAFADTHLSARV
                     ERRAGTRGVVVAQFPYTPNAEGSGLSAYVPQRVREVLSGTSSWRAELPLGVSGDGLHL
                     SSDLRGTAISLPAPLGKSADDSAGIAVSVASVQDKLLRVHANDAGRLDADIALLASAS
                     GWNTRGVTLNVGPGSAPPAGEGMTITSDAAELDAIEWIAAVRGMLRQPAGEGLALKPV
                     ELKPGRITFAGR"
BASE COUNT          302 a          802 c          838 g          318 t
ORIGIN      
        1 ggctggacga ccagcgccac aacatccgca atgcgcgctt caccctggag cggctcgacg
       61 ccgggaacac gcgtgaaggt ttcgaactcg acggcgcgct gcgcctgccc tcggtgtatg
      121 gcgagagcct gcgcttcgag gccgcggtgc gcgggcacat cggccggccg cagcagtggc
      181 gcggcggctt ccgcctgcgc ggcgacaacc tgctgccgca gatgtggctg cgcgggcacg
      241 tggcgcccgg cacgcgggtg gcggcggaaa acatcggcct cgacctgcag ggcgaactgg
      301 ccggcggcgc gctgtcggcc gtgcggctga cgctgaccag cggcacgctc gcggccagcc
      361 ggcccaggca ggacctgcag ctgcagtcgc tgcgcgctgc gctcagcgcc acgcgcgacg
      421 cagcgggctg gctcctgcgc gtgcagcagc tggatatcga cggcgagacg caggcgcgcg
      481 gcgagctgcg ctacacgccg tcggcgcagg gctatgcagt gaaagtcgag gcgccgctgc
      541 tcaggcttga ccgcattgcg ccctggctcg gctttgcgcg cacgctgccg ggctggctgc
      601 agcatgcggc cggcgccagc ggcgagatcg acgacgcggt gctcgacctg cgccaggacg
      661 gcgacgcgcg ccactacgcc gcgcgcgcca gcctccggca attgcagttc gccagcagcg
      721 acgggctgcc gggtgtcggc ggaatttcag gcgaactctc cgccgacgag aacggcggcc
      781 gctttacgct cggcggcggc ggcgttgcgc tggacctgcc ggcgacgttc gccgcaccgg
      841 tccagctcga ccagctggcc ggcgaggtga gctggcagca ggtgcccgag ggctggcaga
      901 tctcggcgcc ggtgctcgac tggcgcctgg ccggcggcgc cgggcgtgcg cgcgtgggcc
      961 tgctgctgcc cgctggcgag cgctcgccgg aactcgactt gggcgcgtcg ttcgcctttc
     1021 ccgacgccgt tgcactcaag gggctgatgc cgctgcactg gagcgagggc ctgcgcagtt
     1081 ggcttgaccg cggcatcgtc gctgcgcgca tcccgtcggc gcggctggcg atccacggtg
     1141 cgctggccga ttttcccttc gtcgacggca agcccggcga gtggaagctc gacgtcagcg
     1201 tggccggcgc cacgctggcc tacgcggcgc gctggccgca ggcggacaac ctgcgcgcgc
     1261 agctgcattt ccgcggcaac agcctgtcga tcgaatccga cggcgctgat ctcggcggcg
     1321 tgcacgcgcg gcgcatcaat gccgagttcg gcgatttccg cgagagccac ctggccatcg
     1381 acggccaggt cagcggcgag atggtgcgct actacagctt cctgcagaac tcgccgctgc
     1441 gcgaaaccct gtcgggcctg ctcgacaaca cgcgcgctgc cgggccggcc gaggtatcgc
     1501 tgcacctgga cattccgctg cgcgatgccc agcgcaccgc ggccagcggg ctgatccggc
     1561 tcgacggcgt ggagctgtac tacggctcgc tggccgatcc tgtttccgac atccatggcg
     1621 agctggcctt cgaccaggat ggcgtcagcg ggcaggacct gaccgccgct tttgccgaca
     1681 cgcacctgtc ggcgcgcgtc gagcggcgcg ccggcacgcg tggcgtggtg gtggcgcagt
     1741 tcccgtacac gcccaatgcc gagggcagcg gcctgtcggc ctacgtgccg cagcgtgtgc
     1801 gtgaagtgct gtctggcacc agcagctggc gcgcggaact gccgctgggc gtgagcggcg
     1861 acggcctgca cctgtcgagc gacctgcgcg gcaccgccat cagcctgccg gcgccgctgg
     1921 gcaagtccgc cgacgacagc gccggcatcg ccgtctcggt ggcgtccgtg caggacaagc
     1981 tgttgcgcgt gcacgccaat gatgccgggc ggctcgacgc cgacatcgcg ctgcttgcgt
     2041 cggcgtccgg ctggaacacg cgcggcgtga cgctcaacgt cggccccggc agcgctccgc
     2101 cggcgggcga gggcatgacc atcaccagcg acgcggcgga actcgacgca atcgagtgga
     2161 tcgccgccgt gcgcggcatg ctgcggcagc cggcgggcga agggctggcg ctgaaaccgg
     2221 tggagctcaa acccgggcgc atcacgtttg ccggccgcta
//
LOCUS       DASZZC010000111         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_586654,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000111 DASZZC010000000
VERSION     DASZZC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586654"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VHE37_02660"
     CDS             <1..918
                     /locus_tag="VHE37_02660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT34458.1"
                     /translation="LRDFFVARFARIYPLHFATLMLMVLIELGRWALERHGYATLNSP
                     AFSYATQLRYLPYDITLTHAWGFLPQQTWNVPSWSISAETFCYLAYPLVLRAGVGRGR
                     TATITAWTCSLGALAGLQWWRGGLDLTYDFSPLRCLPEFVIGSLLYQHTRAHHHLLVN
                     RQWKREALPVAACLLVLLSMWMQTTDIVVVTALAMMVITCADNRSRLARVLSLKPLHY
                     LGDISYSVYLLHHLVLVLFIVVLMVNQGSVADFLLEHSTVTIMLACLTAVVLAHFSYQ
                     RLERPAQRWLRRWLGRRRAPEPAAAPASG"
     gene            944..2092
                     /locus_tag="VHE37_02665"
     CDS             944..2092
                     /locus_tag="VHE37_02665"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT34459.1"
                     /translation="MSSTARPTEFIALTSLRGVAALGVVYFHSYIYFWNYATPHDWTC
                     PKFYLWVDFFLVLSGFVMAHAYGDKLRASGSAPLAEFALARIARVYPLHLLMLCVMMV
                     IEAVRWRMAAAGIDPLQRPAFSGPTEWRALPYNLLLVHAWNLLPRLTWNVPSWSISAE
                     AACYLAFPLLVRSGLDRQRWSTALLALVSLGGLAVLDHRYGGLNQTFHNSVLRCLPEF
                     ILGWLVYRAAHSRMAESLPPLLREALPWLVCALVIAGFACDADDFVMACGFVLLVYAY
                     AANGTRAARLMAARPLHWLGDISYSVYLTHHALLLALMLWLGASHDPLREWACAHPVV
                     LIAGAMLLVIGCSHVSYRAVEKPAQRWLRGLRGRRAAPLAAAPAQSQA"
     gene            complement(2096..2491)
                     /locus_tag="VHE37_02670"
     CDS             complement(2096..2491)
                     /locus_tag="VHE37_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34460.1"
                     /translation="MSKTATTFVAVIIQIMIIDIVFSLDSVITAVGMTNRLPVMVAAI
                     VVAVFVMMLLSGPLSRFVDRHPTIKILALSFLILIGMALVGEGFGQHVPKGYIYFAMA
                     FSTIVEMVNLKTRKPHKRNLPPPGDASGV"
     gene            complement(2491..2844)
                     /locus_tag="VHE37_02675"
     CDS             complement(2491..2844)
                     /locus_tag="VHE37_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HVT34461.1"
                     /translation="MHALFDPDIWLALFTLAALEIVLGIDNIIFLTIMANKLPEEQRA
                     RARTLGLAGAMLTRILLLFSITWLARLTTPLFTLMEHDFSGRDLVLIAGGMFLIWKST
                     MEIHQHVEGDDEGGV"
     gene            complement(2861..3547)
                     /locus_tag="VHE37_02680"
     CDS             complement(2861..3547)
                     /locus_tag="VHE37_02680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HVT34462.1"
                     /translation="MRVHWLQHAEHEELGSIAPWLAARGDAVTCTRLYAGETLPAADA
                     FEWLIVMGGPMNIYEYDVHPWLRDEKQFIRAALEAGRRMLGICLGAQLIADVAGGKVT
                     RNAHAEIGWFDVQLNEGAPAAPAFAGFPPRFTAFHWHGDTFAIPPGARALMSSEACAN
                     QAYALGPRVAAIQFHLEVTAADARAWLDLEQPAPARYVQPREEILRDVGRFAANNRLM
                     LQLLERLAAA"
     gene            complement(3544..3939)
                     /gene="msrB"
                     /locus_tag="VHE37_02685"
     CDS             complement(3544..3939)
                     /gene="msrB"
                     /locus_tag="VHE37_02685"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HVT34463.1"
                     /translation="MDRITKPDSEWRAQLTPEQYRVARQHGTEMPHSGEYDQQFDPGT
                     YKCIGCGAPLFESLAKFDAGCGWPSFFQPLSGAPVEESHDSSHGMVRTEVHCARCGCH
                     LGHVFDDGPRPTGLRYCINSVSLKFEPKK"
     gene            3975..>4535
                     /locus_tag="VHE37_02690"
     CDS             3975..>4535
                     /locus_tag="VHE37_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HVT34464.1"
                     /translation="MITILCVAAALCAIAAIAAEWNERRHGSFYLLKPLTTLLIIGIA
                     LCGAPSEYRQLVLLALVLSLAGDVCLMFEGDGWFIGGLGSFLVAHVLFVAAFAHGIPG
                     NGIPWWSAVFVLYGIGFFGWLLPKTGKLMPAVIIYGIALLSMAIAASARWATLLDARA
                     ELALAGAVLFIVSDSSLAVRKFSGPYA"
BASE COUNT          671 a         1502 c         1512 g          850 t
ORIGIN      
        1 ctgcgcgatt ttttcgtggc gcgctttgcc cgcatctacc cgctgcactt cgcgacgctg
       61 atgctgatgg tgctgatcga actcggccgc tgggcgctgg aacgtcacgg ctacgcgacg
      121 ctgaactcgc cggccttcag ctacgccacg cagctgcgct acctgccgta cgacatcacg
      181 ctcacccacg cctggggttt cctgccgcaa cagacctgga acgtgccgtc gtggagcatc
      241 agcgccgaga ccttctgcta tctggcgtac ccgctggtgc tgcgcgccgg cgtcggccgg
      301 gggcgcaccg ccaccatcac cgcctggacc tgctcgctcg gcgcgctggc cggcctgcaa
      361 tggtggcgcg gcgggctcga cctcacctat gacttctcac cgctgcgctg cctgccggag
      421 ttcgtcatcg gttcgttgct gtaccagcac acacgcgcgc accatcacct gctggtcaac
      481 cgccagtgga agcgggaggc gctgccggtg gccgcatgcc tgctggtatt gctgtcgatg
      541 tggatgcaga ccaccgacat cgtggtggta actgcgctgg ccatgatggt gatcacctgc
      601 gccgacaacc gcagccggct ggcgcgcgtg ctgtcgctga aaccgctgca ttacctcggc
      661 gacatctcct actcggtgta cctgctgcat cacctggtgc tggtgctgtt catcgtggtg
      721 ctgatggtca accagggcag cgtcgcggat ttcctgctcg agcacagcac cgtgaccatc
      781 atgctcgcct gtctcacggc ggtggtgctg gcgcattttt cctaccagcg gctggaacgc
      841 cccgcgcagc gctggctgcg ccgctggctg ggccgccgcc gcgcgcccga gcctgccgct
      901 gcgccggcat ccggttgaac tccggtagca tggcgccctg gccatgagca gcaccgcccg
      961 tcccaccgag ttcatcgccc tgaccagcct gcgcggcgtg gccgcgctgg gcgtggtgta
     1021 tttccacagc tacatctact tctggaatta cgcaacgccg cacgactgga cctgtccgaa
     1081 gttttatctc tgggtggact tcttcctggt gctgtcgggc ttcgtcatgg cccatgccta
     1141 tggcgacaag ctgcgggcga gcggcagcgc accgctggcg gaattcgcgc tggcgcgcat
     1201 cgcccgcgtc tacccgctgc acctgttgat gctgtgcgtg atgatggtga tcgaagccgt
     1261 gcgctggcgc atggcggccg ccggcatcga tccgctgcag cgtcccgcct tttccggccc
     1321 caccgaatgg cgcgcgctgc cctacaacct gctgctggtg cacgcgtgga acctgctgcc
     1381 gcggctcacc tggaacgtgc cgtcgtggag catcagcgcc gaagccgcgt gctacctggc
     1441 gttcccgctg ctggtgcgca gcggactgga ccgtcaacgc tggagtacag cgctgctcgc
     1501 actggtttca ctcggcggac tggccgtgct ggaccatcgc tacggcgggc tcaaccagac
     1561 cttccacaac tcggtgctgc gctgcctgcc ggaattcatc ctcggctggc tggtatatcg
     1621 cgccgcccac agccgcatgg cggaatcgct gccaccgctg ctgcgcgaag cgctgccatg
     1681 gctggtctgc gcgctggtca tcgccggctt tgcctgcgac gccgatgatt tcgtcatggc
     1741 ctgtggcttc gtgctgctgg tttatgccta cgccgccaac ggcacgcgtg cggcgcgctt
     1801 gatggcggcg cggccgctgc actggctggg cgatatttcc tactcggttt acctcacgca
     1861 ccacgcgctg ctgctggcgc tgatgctgtg gctgggcgcc agccacgatc cgctgcgcga
     1921 gtgggcctgc gctcacccgg tggtgctgat cgccggggcc atgctgctgg tcatcggctg
     1981 ctcgcacgtc agctaccgcg cggtggaaaa gccggcgcaa cgctggctgc gcggtttgcg
     2041 cggccgccgc gcggcgccgc tcgccgcagc gcctgcgcaa tcgcaggcct gacagctaca
     2101 cgccgctggc gtcgcccggc ggcggcagat tgcgcttgtg cggcttgcgc gttttcaggt
     2161 tgaccatttc tacgatcgtg ctgaaggcca tggcgaaata gatgtagccc ttgggcacgt
     2221 gctggccgaa gccttcgccg accagcgcca tgccgatcag gatcaggaac gacaacgcca
     2281 ggatcttgat ggtggggtgg cggtcgacga agcgcgacag cggcccggac aacaacatca
     2341 tcacgaacac cgccaccaca atcgctgcaa ccataactgg aagccggttc gtcatgccga
     2401 cagcggtgat gaccgaatcc agtgagaaca cgatgtcgat gatcatgatt tggatgatca
     2461 cagcgacaaa ggtcgtggcg gtcttgctca ttacactcca ccttcatcgt cgccttcgac
     2521 gtgctgatga atttccatgg tgctcttcca gataaggaac attccgcctg cgattagcac
     2581 taggtcacgt cccgaaaagt cgtgctccat gagggtgaag agaggcgtag ttagtcgcgc
     2641 gagccaagtg atcgaaaaca gcaacaggat gcgcgtgagc atggcgccgg ccaggcccag
     2701 cgtgcgggcg cgcgcgcgct gctcttccgg cagcttgttg gccatgatgg tgaggaagat
     2761 gatgttgtcg atgcccagca cgatctccag cgcggcgagc gtgaagaggg cgagccagat
     2821 gtcgggatcg aacagggcgt gcatgcgcgc aggataaccg ttatgccgcg gccagacgct
     2881 cgagcagctg cagcatcagc cggttgttgg cggcgaagcg gccgacatcg cgcaggatct
     2941 cctcgcgcgg ctgcacatag cgcgcaggtg cgggctgctc cagatcaagc cacgcgcgcg
     3001 catcggccgc ggtcacttcg agatggaact ggatcgcggc cacgcgcgga cccagcgcgt
     3061 aagcctggtt ggcgcaggct tcgctgctca tcagtgcgcg ggcgcccggc gggatggcga
     3121 aggtgtcgcc gtgccagtga aacgcggtga agcgcggcgg aaaaccggcg aaggccggcg
     3181 ctgccggcgc gccctcgttg agctgcacgt cgaaccagcc gatctccgcg tgcgcgttgc
     3241 gcgtgacctt gccgccggcc acgtcggcga tcagctgcgc gcccaggcag atgccgagca
     3301 tgcgcctgcc ggcttcaagc gcggcacgga tgaattgctt ttcgtcgcgc agccaagggt
     3361 gcacgtcgta ttcgtagatg ttcatcggcc cgcccatgac gatgagccac tcgaacgcgt
     3421 cggccgccgg cagcgtctcg ccggcgtaca ggcgcgtgca ggtgaccgcg tcgccgcgcg
     3481 cggcgagcca cggcgcaatg ctgccgagct cttcatgctc ggcgtgctgc agccaatgca
     3541 ccctcacttc ttcggctcga acttcagcga caccgagttg atgcagtagc gcaggccggt
     3601 cggccgcggg ccgtcgtcga acacgtggcc caggtggcag ccgcagcgcg cgcaatgcac
     3661 ttccgtgcgc accatgccgt ggctgctgtc gtggctttcc tccaccggcg cgccggacaa
     3721 cggctggaag aagctgggcc agccgcagcc ggcgtcgaac ttggccagcg actcgaacag
     3781 cggggcgccg cagccgatgc acttgtacgt gccgggatca aattgctggt cgtactcgcc
     3841 gctgtgcggc atctcggtgc cgtgctggcg cgccacgcgg tactgctcgg gcgtgagctg
     3901 cgcgcgccac tcgctgtcgg gtttggtaat ccggtccatg cgtcctatcc tgtgtgctcg
     3961 gaactggagc ggcgatgata acgatcctgt gcgtggctgc ggcgctgtgc gccatcgccg
     4021 ccatcgcggc ggaatggaac gagcggcgcc atggcagctt ctacctgctc aagccgctga
     4081 ccacgctgct gatcatcggc atcgcgctgt gcggtgcgcc cagcgaatac cgccagctgg
     4141 tgctgctggc gctggtgctg tcgctcgccg gcgatgtgtg tttgatgttc gagggcgatg
     4201 gctggttcat cggcgggctc ggcagtttcc tggtggcgca cgtgctgttc gtggccgcgt
     4261 tcgcgcatgg catacccggc aacggcatcc cgtggtggag cgcggtgttc gtgctctacg
     4321 gcatcggctt cttcggctgg ctgctgccga agaccggcaa gctgatgccg gcggtgatca
     4381 tctacggcat cgcgctgctg agcatggcga tcgccgccag cgcgcgctgg gccacgctgc
     4441 tcgacgcgcg cgccgagctc gcgctggcgg gcgcggtgct gttcatcgtg tcggattcgt
     4501 cgctggcggt gcgcaagttc agcgggccgt atgcg
//
LOCUS       DASZZC010000112         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1102844,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000112 DASZZC010000000
VERSION     DASZZC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102844"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VHE37_02695"
     CDS             complement(<1..510)
                     /locus_tag="VHE37_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34465.1"
                     /translation="MLAWMVTRTDWYVTMSLCGAAIVLQMLGLGRYAARTGREVGRFL
                     DAIAFDDTSTGFAGLSRDRNFADLGQAMTRVLEQLRSGRVEREEQAQYLQSLLAHMPV
                     ALISVSERGSVQLLNLAARRLFAGPCESTAGLARYGAAFATALDALKPGQTAIVRMER
                     SAGAMQLKAA"
     gene            complement(581..>1512)
                     /locus_tag="VHE37_02700"
     CDS             complement(581..>1512)
                     /locus_tag="VHE37_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT34466.1"
                     /translation="ATQTDSGMIGTSPAMLKVFGAIRRTAPTDANVLLLGENGTGKEL
                     AAREIHRLSSRASEVFLRVDMGALSPQLFESELFGHKRGAFTDARADRTGYFRAATGG
                     TLFLDEIGNVPLALQSKLLTVLEQREVTPVGAEKPEPIDVRLVCATNMGRDRLADENL
                     FRPDLLYRINTVEIVLPPLRERPQDIPLLLEHYAAQYSQKYNLPPKRLSAALLDRLSA
                     WSWPGNVRALRHAMERAVILSEGDLLDVTDFPLAASERGAPAASAISPLDEVEKNAIV
                     RALEKHQNNVSRAAEALGLTRASLYRRMEKYGL"
BASE COUNT          249 a          497 c          519 g          247 t
ORIGIN      
        1 ggcggccttg agctgcatcg cgccggcgga tcgctccatc cgcacgatcg cggtctggcc
       61 gggcttgagc gcatccagcg cggtggcgaa agccgcgccg tagcgcgcca atcctgcggt
      121 cgattcacag ggcccggcga acagccgccg cgccgccagg ttcagcagtt gcacgctgcc
      181 gcgttcactg actgaaatca gcgccaccgg catgtgcgcc agcaggcttt gcagatactg
      241 cgcctgttcc tcgcgctcca cccggccgct gcgcaactgt tccagaaccc gcgtcatggc
      301 ctggcccaga tctgcgaaat tgcggtcgcg cgacaggccg gcaaagccgg tcgaggtatc
      361 gtcaaaggcg atggcgtcca ggaagcgccc cacctcgcgg ccggtgcgcg ccgcataacg
      421 gccgagcccc agcatctgca gcacgatggc ggcgccgcac agcgacatgg tgacatacca
      481 gtcggtgcgc gtgaccatcc aggccagcaa cgccagtgtc gcctgcaacc ccgccagcct
      541 gagcaggatg ccggtctgga aattgttgag acgaaatccg ctagagccca tacttttcca
      601 tccgccggta gagcgaggcg cgggtcaggc ccagcgcttc cgcggcgcgg ctgacattgt
      661 tctggtgctt ttccagggcg cggacaatgg cgttcttttc cacctcgtcg aggggcgaaa
      721 tggcggatgc cgccggcgcg ccgcgttcgc tcgcggccag cgggaaatcc gtgacgtcca
      781 gcaggtcgcc ttcggaaagg atcacggcgc gctccatggc gtggcgcagc gcgcgtacat
      841 tgcccggcca ggaccaggcc gagagccggt cgagcagcgc cgcggacagg cgcttgggcg
      901 gcagattgta tttctgagaa tattgcgcgg cataatgctc cagcagcagg ggaatgtcct
      961 gcggccgttc gcgcaacggc ggtaggacga tctcgaccgt gttgatgcga taaagcaggt
     1021 cggggcggaa caggttttcg tcggccaggc gatcgcggcc catattggtg gcgcagacaa
     1081 gccgcacatc gatgggttcg ggcttttcgg cgcccaccgg cgtcacctcg cgctgctcca
     1141 gcacagtgag cagcttggat tgcagcgcca gcggcacgtt gccgatctcg tccaggaaca
     1201 gcgtgccgcc cgtggcggcg cggaaatagc cggtgcggtc ggcgcgcgca tcggtgaagg
     1261 cgccgcgctt gtggccgaac aattcacttt cgaacagttg cggcgacagc gcgcccatgt
     1321 cgacgcgcag gaacacctcg ctggcgcgtg acgacaggcg gtggatctcg cgcgccgcca
     1381 gttccttgcc ggtgccgttt tcgcccagca gcagcacatt ggcgtcggtc ggcgcggtgc
     1441 ggcggatggc gccgaacacc ttcagcatgg ccggcgaggt gccgatcatg ccgctgtcgg
     1501 tctgggtggc gg
//
LOCUS       DASZZC010000113         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_909442,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000113 DASZZC010000000
VERSION     DASZZC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909442"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /locus_tag="VHE37_02705"
     CDS             complement(<1..2540)
                     /locus_tag="VHE37_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34467.1"
                     /translation="MNRSVLLCVLALAGCHGASTDNGSGPIPGDGTAYQAFVSKALPV
                     LRAQCAPCHAGARFGFASVTGDDAADYAKFFPMLSLDQPAQSRLLAKILPASDPNSIA
                     HAGGARVSGTGDPNYQQLMQWVNAEVAEKCAGGCGMGAPRQWVAYVQQPDFFWSTSKI
                     PSRTDWGIRSGARIMLQKVQPGGALIAPTADAAVDFLLGSGFCENGDCDFGNLSVSYQ
                     GTQMAFECRMQVAGESYVERSWNICIAGVDLATGKSINPHRLMSAPNYGYTYARATPL
                     AIACFARQTSYPFACNNYGPDPNGLSFALGGFYDRHGMFRQRHDLTPVFTPDGTRVIF
                     ASMRPDPRSGEDSVQSYHGNTHLLNLVSAALDGSDLRTLYRNEGGVADLPFFLKNGNL
                     AFHVWLLDRTDRHMYLQSSADGMVELPTLMGHTQGPNMWGKATQLNDGLILGLTGNRR
                     GAANNVQPFIADHTLGSNLDPSFVSEKQIGPDVLGQFQAFPNSYCTSPPDGPNCSTSQ
                     YFADANWSPDGRALVVQNTSKTYMGNFNDNQNEDGESFYTTYNSLDPLNYLPQDMGIS
                     MIDKSGAVQVFIANPAGTILRYPVWVGRRQPPTPQSAVTNEAAHTATLHIADFKIWLG
                     LQQNAPDKTSDMQSLAGIKSVRVLYKEMSGNACLSDGATRDMTNTGFDFDHPVAMGAN
                     NASGFIKYDVPVAAGGDGHGDIPLKADNSVLLTIPSGRMLYFQGVDQNGFVVAQRSHL
                     FSLPPRPAGAANIINTSVPTAQYDSQCIACHGSIDGSSYVGLAAFGNQALVPLDFTRT
                     QAYQALPVDLSSAAVGIRTMTFKDQLRPILDAKCVSCHQGENPAGEFSL"
     gene            complement(2653..3609)
                     /locus_tag="VHE37_02710"
     CDS             complement(2653..3609)
                     /locus_tag="VHE37_02710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT34468.1"
                     /translation="MIYRNEQDQILGALDASRHEQLIAGEPARYTEMVRNITGYGVHL
                     VDREGRIQSWNRGAGIITGYAPQDVIGQPYSTVFAESALKEGAPGRTLNFARANRHCR
                     DQHLRKKRNGGEFLAHATLDAVRGADGQIIGFVEVFQDVTEQKQREDRLYQRATRDAL
                     TGLFNRGHFTEMGTQEIERARRFAEPLSIVLMDIDHFKKINDTWGHETGDQALIALAN
                     AVNGFIRKIDFVGRLGGEEFALALPRANKEPALDMAQRLRLHIAALKLGSAGKEFSFT
                     ISMGIAALRPDTRDLQELLRNADAALYRAKREGRNRVEVWFE"
     gene            3834..>4465
                     /gene="rarD"
                     /locus_tag="VHE37_02715"
     CDS             3834..>4465
                     /gene="rarD"
                     /locus_tag="VHE37_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HVT34469.1"
                     /translation="MQQGLLAAAAAYFIWGLFPIYWRYLVEVPSLQIMAHRIVWCALL
                     VFAYLFARDGRAWLARFNRKLLLMLSGSSLLIAVNWWLYIWAVNHGHIVETSLGYFIN
                     PLVSVLLGVFLLGERLNTTQRVAVVIAAGGVLYLTWSAGHPPLIALALAFSFGGYGLI
                     RKLAVVPSIEGLAVESGLLFIPALLLLLLCEHDGSGVFGHASTRTNVLLVL"
BASE COUNT          678 a         1401 c         1544 g          842 t
ORIGIN      
        1 aggctgaact cgccggcggg attctcaccc tggtggcagg acacgcattt cgcatcgagg
       61 atcgggcgca gctgatcctt gaaggtcatg gtcctgatgc cgaccgcagc ggatgagaga
      121 tccaccggca gcgcctggta agcctgtgtg cgcgtgaaat cgagcggcac cagcgcctgg
      181 ttgccgaacg ccgccaggcc gacgtaggac gaaccgtcga tcgagccgtg gcacgcgatg
      241 cactgcgagt cgtactgcgc ggtcggcacc gaggtgttga tgatgtttgc cgcgcccgcc
      301 ggccgcggcg gcagcgagaa caggtggctg cgctgcgcga cgacgaagcc gttctggtcc
      361 acgccctgga aatacagcat gcggccggac gggatggtga gcagcaccga gttgtccgcc
      421 ttcagcggga tgtcgccgtg gccatcgccg ccggcggcga ccggcacgtc gtacttgatg
      481 aagccgctgg cgttgttggc gcccatcgcc accgggtggt cgaaatcgaa gccggtgttg
      541 gtcatgtcgc gcgtggcgcc atccgacagg caggcgttgc cgctcatttc cttgtacagc
      601 acgcgcaccg acttgatccc cgcgagcgat tgcatgtcgc tggtcttgtc gggcgcgttc
      661 tgctgcagcc cgagccagat cttgaagtcg gcgatgtgca gcgtggcggt atgcgcggcc
      721 tcgttggtca ccgcggattg cggagtcggc ggctggcggc gtcccaccca caccgggtag
      781 cgcaggatgg tgcccgccgg attggcgatg aacacctgca cggcgccgct cttgtcgatc
      841 atcgagatgc ccatgtcctg cggcaggtag ttcagcgggt ccagcgagtt gtaggtggtg
      901 tagaacgatt cgccgtcctc gttctggttg tcgttgaagt tgcccatgta agtcttgctg
      961 gtgttctgca cgaccagcgc gcgcccgtcc ggcgaccagt tcgcgtcggc gaaatactgc
     1021 gaggtggagc agttcggccc gtcaggcggc gacgtgcagt agctgttggg aaacgcctgg
     1081 aactggccga gcacatccgg gccgatctgt ttttcgctga cgaaactggg gtcgagattc
     1141 gagcccagcg tgtggtcggc gatgaacggc tgcacgttgt tggcggctcc gcggcggttg
     1201 ccggtgaggc cgaggatcag gccgtcgttc agctgcgtgg ccttgcccca catgttcggg
     1261 ccctgggtgt ggcccatcag cgtgggcagt tccaccatgc cgtcggcgct gctttgcagg
     1321 tacatgtgcc ggtcggtgcg gtcgagcagc catacatgaa acgccaggtt gccgttcttc
     1381 aggaagaacg gcaggtcggc cacgccgccc tcgttgcggt agagggtgcg caggtcgctg
     1441 ccgtcgagcg ccgccgatac caggttcaac aggtgcgtgt tgccgtgata gctctgcacg
     1501 ctgtcttcac cgctgcgcgg gtcgggccgc atgctggcga agatcacgcg cgtgccgtcg
     1561 ggtgtaaaca ccggcgtgag atcgtggcgc tggcggaaca tgccgtggcg atcgtagaag
     1621 ccgccgagtg caaacgacag cccgttcgga tcagggccat agttgttgca ggcaaatgga
     1681 tagctggtct ggcgcgcaaa gcaggcgatg gccagcggcg tggcgcgcgc gtaggtgtag
     1741 ccgtagttcg gcgccgacat cagccggtgc gggttgatcg acttgcccgt ggcaaggtcg
     1801 acgccggcga tgcagatgtt ccagctgcgc tccacgtacg actcgcccgc cacctgcatg
     1861 cggcactcga acgccatctg cgtgccctgg tagctcaccg acaggttgcc gaaatcgcag
     1921 tcgccgtttt cgcagaagcc cgagcccagc aggaagtcga ccgccgcgtc ggcagtgggc
     1981 gcgatcagcg cgccgccagg ctgcaccttc tgcagcatga tgcgcgcacc actgcggatg
     2041 ccccagtcag tgcgcgatgg aattttcgag gtggaccaga agaaatccgg ctgctgcaca
     2101 tacgccaccc actgccgcgg cgcgcccatg ccgcaaccgc cggcgcattt ctccgccacc
     2161 tcggcgttca cccactgcat cagctgctga tagttgggat caccggtgcc ggaaacgcgc
     2221 gcgccgccgg cgtgcgcaat cgagttgggg tcggaggccg gcaggatctt cgccagcagg
     2281 cggctctgcg ccggctggtc gagcgacagc atcgggaaaa atttggcgta gtccgcggcg
     2341 tcgtcgccgg tcacgctggc gaaaccgaag cgcgcgccgg catggcaggg cgcgcattgt
     2401 gcgcgcagca ccggcagcgc cttgctgacg aatgcctggt aagcggtgcc atcgccggga
     2461 atcgggccac tgccgttatc agttgatgcg ccatggcagc ccgccagcgc gagcacgcac
     2521 agcaggactg atctgttcac cacccgggct ccccacgacg tatgcggccc actgttcgcc
     2581 gcggccgggc cagcgtcaag caaggggacg gcaagcggtg tgattgccgt aacggacggc
     2641 aggccggcgc atctactcga accacacttc cacgcggttg cgcccctcgc gcttggcgcg
     2701 gtaaagcgcg gcatcggcat tgcgcagcag ttcctgcagg tcgcgcgtgt ccgggcgcag
     2761 cgcggcgatg cccatgctga tggtgaagga aaattccttg ccggcgctgc cgagcttgag
     2821 cgcggcgatg tgcaggcgca ggcgctgcgc catgtccagc gccggttcct tgttggcgcg
     2881 cggcaatgcc agcgcgaatt cctcgccgcc gaggcggccg acgaaatcga tcttgcggat
     2941 gaagccgttg accgcattgg ccagcgcgat cagcgcctgg tcgccggtct cgtggcccca
     3001 ggtgtcgttg atcttcttga agtggtcgat gtccatcagc acgatcgaca gcggctcggc
     3061 gaagcgccgc gcgcgctcga tctcctgcgt gcccatctcg gtgaaatggc cgcggttgaa
     3121 caggccggtg agcgcatcgc gcgtggcgcg ctggtacagg cggtcctcgc gctgcttctg
     3181 ctcggtgacg tcctggaaca cctcgacgaa gccgatgatc tggccatcgg cgccgcgcac
     3241 cgcgtcgagc gtggcatgtg ccaggaactc gccgccgttg cgcttcttgc gcaggtgctg
     3301 gtcgcggcaa tggcggttgg cgcgggcgaa gttgagcgtg cggccgggcg cgccttcctt
     3361 cagcgcgctc tcggcgaaca ccgtcgaata cggctggccg atgacgtcct gcggcgcgta
     3421 gccggtgatg atgccggcgc cgcggttcca gctctggatg cggccttcgc ggtcgaccag
     3481 gtgcacgccg tagccggtga tgttgcgcac catctcggta tagcgcgcgg gctcgccggc
     3541 gatcagctgc tcgtggcggc tggcgtcgag tgcgccgaga atctggtctt gttcgttgcg
     3601 gtagatcacg gtgttcgctt gcgcggcccg ggcattctaa cttctgcgaa tttgcttacg
     3661 ctgccgccgg caccggcaaa aatggcctgg ccgcactgcc cccggcgatg ttattaaata
     3721 ttgtatatat ataaaataat acattattat ataacatggc aagtgccggt gcggtgcagg
     3781 gcaaaacggc gtcgtgagct atgctgcgcg cccttttttc ttcgcagcgt gtcatgcagc
     3841 agggcttgct cgccgccgcc gcggcgtact tcatctgggg attgttcccg atctactggc
     3901 gctacctggt cgaggtgccg tcgctgcaga tcatggcgca tcgcatcgtc tggtgcgcgc
     3961 tgctggtgtt cgcctacctg ttcgcgcgcg acggccgcgc ctggctggcg cgcttcaacc
     4021 gcaagctgct gctgatgctc agcggcagtt cgctgctgat cgcggtgaac tggtggctgt
     4081 atatctgggc ggtgaaccac gggcatatcg tcgagaccag cctgggctat ttcatcaacc
     4141 cgctggtgtc ggtgctgctc ggcgtgttcc tgctgggcga gcggctcaac accacgcagc
     4201 gcgttgcggt ggtgatcgcc gcgggcggcg tgctgtacct gacctggagc gccggccacc
     4261 cgccgctgat cgcgctggcg ctggcgtttt ccttcggcgg ctacgggctg atccgcaagc
     4321 tcgcggtggt gccgtcgatc gagggcctgg cggtcgagag cgggctgctg ttcatccccg
     4381 cgctgctgct cctgctgctg tgcgagcacg acggcagcgg cgtcttcggc cacgccagca
     4441 cgcgcaccaa cgtgctgctg gtgct
//
LOCUS       DASZZC010000114         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1102954,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000114 DASZZC010000000
VERSION     DASZZC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102954"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VHE37_02720"
     CDS             complement(<1..1213)
                     /locus_tag="VHE37_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HVT34470.1"
                     /translation="MKIFATTFATETNTFAVVPTGRVDFEAYGVFRGDGSVKAANEMG
                     AFHAELHRLAARDGHTVVESLVAFAQPSGYTLRAVYEEYRDQILEDLKAAMPVDAVQL
                     ILHGAMVAHGYDDCEGDLISRVRKIVGPKVPIGVELDLHCHFTELMRSSADIIICYKE
                     YPHTDGRERLHEVYHLTMQQAAGRIRPVTAMVDTRMVGLWHTTSEPMVSFVKRMQSFE
                     GKDGILSVSLGHGFPWGDTPDTGARLWVVADGDMARAQALAQQLAREFWDMRNESYTP
                     PVPLKDAVARALAARRGPVVLADVGDNAGGGSMSDSTYILRALLDAGARDVALGCYWD
                     LGAVALCRSAGIGATLDLRIGGKCGPLSGQPVDLRVTVRAIVENHGQHALSVRYPMGT
                     AVWVQADNGIDI"
BASE COUNT          251 a          513 c          508 g          287 t
ORIGIN      
        1 cgatgtcgat gccattgtcc gcctgcaccc agacggcggt gcccatcggg taacgcacgc
       61 tgagcgcgtg ctggccgtgg ttctccacga ttgcgcgcac cgtcacgcgc aggtcgaccg
      121 gctggcccga cagcggcccg cacttgccac cgatgcgcag atccagcgtg gcgccgatgc
      181 cggcgctgcg gcagagcgcg accgcgccca ggtcccagta gcagccgaga gcgacatcgc
      241 gtgcgccggc gtcgagcagc gcgcgcagga tgtaggtgct gtcgctcatg ctgccgccgc
      301 cggcgttgtc gccgacgtcg gccagcacca ccggcccgcg cctggcggcc agcgcgcgcg
      361 cgaccgcgtc cttcagcggc accggcggcg tgtagctttc gttgcgcatg tcccagaact
      421 cgcgcgccag ctgctgcgcc agcgcttgtg ccctggccat gtcgccgtcg gccaccaccc
      481 acaagcgcgc gccggtgtcg ggcgtgtcgc cccagggaaa accgtggccg agcgatacgc
      541 tgaggatgcc gtccttgcct tcgaacgact gcatgcgttt gacgaacgac accatcggtt
      601 cgctggtggt gtgccacagc ccgaccatgc gcgtgtcgac catcgccgtc accggcctga
      661 tcctgccggc cgcctgctgc atggtgaggt gatagacctc gtgcaggcgc tcgcggccat
      721 cggtgtgcgg atattccttg tagcagatga tgatgtcggc gctgctgcgc atcagctcgg
      781 tgaagtggca atgcaggtcc agctccacgc cgatcggcac cttgggcccg acgattttgc
      841 gcacgcggct gatcaggtcg ccttcgcaat cgtcgtagcc atgcgccacc atggcgccgt
      901 gcaggatcag ctgcaccgcg tcgaccggca tcgcggcctt gaggtcttcg aggatctggt
      961 cgcggtattc ctcgtacacc gcgcgcagcg tgtagccgga aggctgcgcg aaggccacca
     1021 ggctctccac caccgtgtgt ccatcccgcg ccgccagccg gtgcagttcg gcatggaagg
     1081 cgcccatttc gttggccgcc ttgacgctgc cgtcgccgcg gaaaacgccg taggcctcga
     1141 agtcgacgcg gccggtcggc acgacggcaa acgtattggt ctcggtggcg aaggtggtgg
     1201 cgaagatctt catccgggca tccgtctcaa gtgacttgca caaggacaag cttcgccaat
     1261 ccaggccgag ttcgtcatcc gtgaaagcac cggtagctcg gccgcaacgg ccggaaaaac
     1321 gacgcgttca atatctccgt gagcgggctg tgcataagca tctgataagg ggtgcgtatg
     1381 gggtgcaaga atggttgata actacaacta tttgcggtcc atccctggca tggctacaag
     1441 atatagtgtt tcgccttgac aggaccccgc ccgcggcgga tgatttgagc ctgttttttt
     1501 gaggtggaaa cagcggtcgc ggcgccggat tcggccccgc attgttgttt tttgtaccg
//
LOCUS       DASZZC010000115         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_717126,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000115 DASZZC010000000
VERSION     DASZZC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717126"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="flgK"
                     /locus_tag="VHE37_02725"
     CDS             complement(<1..719)
                     /gene="flgK"
                     /locus_tag="VHE37_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HVT34471.1"
                     /translation="MSDLLNIGTSALLAYRSALDTISHNIANANTDGYSRQRVDLEAR
                     PPQVAGNGYVGSGVQVQTTSRVSDSLVNQRLVADNSSYSRLDTFQQMAARADDLLSNS
                     DTGLATPLQNFFSAMDGVAAAPASTAARQTLLSSADTLAGRFGDLSNQLSGMESDING
                     QLSTAVDQINQLAGSIAQLNDRIASGAGSANGNPPNDLLDQRDSLIKQLSSQIGVSTV
                     TKTDGSVDVFAAGGQALVLGSR"
     gene            complement(716..1531)
                     /gene="flgJ"
                     /locus_tag="VHE37_02730"
     CDS             complement(716..1531)
                     /gene="flgJ"
                     /locus_tag="VHE37_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HVT34472.1"
                     /translation="MNPAAASDVASLQGLSQLRLAAKQNDPAALKAAAKQFEAMFMQM
                     LLKSARDAKVGDEAFSGEQSGFYQDMFDQQMAQHLAAGKGLGIADLLVRQLQAKNISA
                     SPQPAASSAPADGTAQKNFISQILPHAQKAAAALGVPAQALVAQAALETGWGQHVVRN
                     ADGSSSNNLFNVKAGSSWSGASATQATTEYVQGRAQTQNAAFRSYDSVGAAFDDYVQL
                     LKNNPRYAQSLHLADTAQFAQSLQQAGYATDPSYAQKITALADGIGRGGSSVA"
     gene            complement(1528..>2072)
                     /locus_tag="VHE37_02735"
     CDS             complement(1528..>2072)
                     /locus_tag="VHE37_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HVT34473.1"
                     /translation="VTLNLHTADFTTATRMAGAINTAIGSAVARAVDPVTVAVAAPTE
                     PDRRISFIATLQNLEVQPGDAPARVVVNSRTGTIVIGGRVRVLPAAVAHGSISVSISE
                     KPEVSQPAPLSKGETTVVPRSTIDVKEEGGHMFLFDAGVSLDDIVRAVNQVGAAPGDL
                     VAILEALKEAGALRAELVVI"
BASE COUNT          280 a          704 c          725 g          363 t
ORIGIN      
        1 cggctgccca gcaccagcgc ctggccgccg gcggcgaaga cgtcgaccga gccgtcggtc
       61 ttggtcacgg tggacacgcc gatctggctc gacagctgct tgatcaggct gtcgcgctgg
      121 tcgagcaggt cgttcggcgg attgccgttg gcgctgcccg caccggaggc gatgcggtcg
      181 ttgagctgcg cgatgctgcc cgccagctgg ttgatctggt cgacggccgt cgacagctgg
      241 ccgttgatgt cgctctccat gccggacaac tggttggaca ggtcgccgaa gcgtccggcc
      301 agcgtgtcgg cggaggacag cagcgtctgc cgcgcggcgg tcgaagccgg cgccgcggcc
      361 acgccatcca tcgcggagaa aaagttctgc agcggcgtag ccaggccggt gtcgctgttg
      421 gaaagcagat cgtcggcgcg cgccgccatc tgctggaagg tgtcgagccg cgagtacgac
      481 gagttgtccg ccaccagccg ctggttgacc agcgaatcgg atacgcgcga agtggtctgc
      541 acctgcacgc cgctgccgac gtagccgttg ccggccacct gcggcggccg cgcttccagg
      601 tccacccgct ggcggctgta gccgtcggtg ttggcgttgg cgatgttgtg gctgatggtg
      661 tccagcgcgc tgcggtaggc cagcagcgcc gaggtgccga tgttcagcag gtcgctcatg
      721 ccacgctgct cccgccgcgg ccgatgccgt cagccagcgc ggtgatcttc tgcgcgtacg
      781 acggatcggt ggcatagccc gcctgctgca gcgactgcgc gaactgcgcg gtgtcggcca
      841 ggtgcagcga ctgcgcgtag cgcgggttgt tcttgagcag ctgcacgtaa tcatcgaacg
      901 ccgcgcccac ggagtcgtag ctgcggaacg cggcgttctg cgtctgcgcg cggccctgca
      961 cgtactcggt ggtggcctgc gtggcgctgg cgccggacca gctgctgccg gccttcacgt
     1021 tgaacaggtt gttgctgctg ctgccgtcgg cattgcgcac cacgtgctgg ccccagccgg
     1081 tttccagcgc ggcctgcgcc accagcgcct gcgccggcac gccgagcgcg gcggcggcct
     1141 tctgcgcgtg cggcaggatc tggctgatga aattcttctg cgccgtgccg tcggccggcg
     1201 cagatgatgc cgccggctgc ggcgacgcgg aaatgttttt cgcctgcagc tggcgcacca
     1261 gcaggtcggc aatgcccagc cccttgcccg cggccaggtg ctgcgccatc tgctggtcga
     1321 acatgtcctg gtaaaagccg ctctgctcgc cggagaatgc ctcgtcgccg accttggcgt
     1381 cgcgtgcgct cttcagcagc atctgcatga acatggcctc gaactgcttc gccgcggcct
     1441 tcagcgccgc cggatcgttc tgcttggcag ccagccgcag ttgcgacagc ccctgcaggc
     1501 tcgccacatc gctggcggcc gctggattca tatcaccacc agctcggcgc gcaatgcgcc
     1561 agcctctttc aaggcttcaa ggatggcgac gaggtcgccc ggcgcggcac ccacctggtt
     1621 gaccgcgcgc acgatgtcgt cgagcgacac gccggcgtcg aacaggaaca tgtgcccgcc
     1681 ttcttccttg acgtcgatgg tcgagcgcgg caccaccgtg gtttcgccct tggagagcgg
     1741 cgcgggctgc gacacttcgg gtttctccga gatcgacacg ctgatcgagc catgcgccac
     1801 cgcggccgga agcacgcgca cgcggccacc gatgacgatg gtgccggtgc gcgaattgac
     1861 caccacgcgc gccggcgcgt cgcccggctg cacctccagg ttctgcagcg tggcgatgaa
     1921 actgatgcgg cggtccggtt cggtcggcgc cgccactgcg acggtcaccg ggtccaccgc
     1981 gcgtgccacc gcgctgccga tggcggtgtt gatcgcgccg gccatgcgcg tggcggtggt
     2041 gaagtcggcg gtgtgcaggt tgagcgtcac tt
//
LOCUS       DASZZC010000116         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_329344,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000116 DASZZC010000000
VERSION     DASZZC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329344"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..327
                     /locus_tag="VHE37_02740"
     CDS             142..327
                     /locus_tag="VHE37_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34474.1"
                     /translation="MELQELIDNFELLGDWGERAALAECLEWHAAPSRARPWTAGLWS
                     YGAGTCATDGTPHVKLA"
     gene            329..739
                     /locus_tag="VHE37_02745"
     CDS             329..739
                     /locus_tag="VHE37_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HVT34475.1"
                     /translation="MNLEELIENFELLGDWEERYRYLIDLGRKLPPLPEGERTEANKV
                     RGCMSQVWLSSKEHAGPPKTLEFSGDSDSHLVKGLIALLFLLVSGKTPQEILATDVSH
                     AFEQLGLSNHITANRRNGFYSMVERIRALAARAA"
     gene            827..1300
                     /locus_tag="VHE37_02750"
     CDS             827..1300
                     /locus_tag="VHE37_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34476.1"
                     /translation="MPGARLAPAPARRFSLWLAALLLLISARSVDAQGDAIEIISSPE
                     HRGVELDRNTLRAIFTMRKREWPDGTPVHVFVLPDTSDVHDQFSREVLGIYPYVLRET
                     WDRMVYTGTGFAPETVNSEAAMRQKVENTPGAIGYLRKPHGSAAVESRKDVRNER"
     gene            1290..>1520
                     /locus_tag="VHE37_02755"
     CDS             1290..>1520
                     /locus_tag="VHE37_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34477.1"
                     /translation="MNARKKSLYRYIGPAAAACAALAALLPGAAAASSLDYQLHGFAA
                     QGLVLSDGNNVLGNSTGGSVAFNEFGLNGAVTL"
BASE COUNT          282 a          511 c          486 g          241 t
ORIGIN      
        1 gcaccgagcc ctcggcgtgg cgggtgaaat gacgctggat gctgacggaa cgcagctggt
       61 ccgcagttct gccggaagga cgcatggaaa agttcgcggg aaacgggcgc ggattatagc
      121 cgtcgctctt aaaataccgg catggagctg caggaactca tcgacaactt cgaactgctc
      181 ggcgattggg gcgagagggc ggctttggcg gaatgccttg aatggcatgc cgcgccctcg
      241 cgcgcccggc catggacggc cgggctgtgg tcttatggcg ccggcacttg cgccacggat
      301 ggcacgccgc acgtaaagct cgcctgatat gaatctcgaa gaactgatcg agaatttcga
      361 actgctcggc gattgggaag agcgttacag atacctcatc gatctcggcc gcaagctgcc
      421 gccgctgccc gagggcgagc gcaccgaagc caacaaggtg cgcggctgca tgtcgcaggt
      481 gtggctgtcc tcgaaggaac atgccggtcc gccgaagacg ctggagttct ccggtgattc
      541 cgattcgcac ctggtgaagg gcctgatcgc gctgctgttc ctgctggtgt cgggcaagac
      601 gccgcaggaa atcctggcca ccgacgtcag ccacgcgttc gagcagctgg gcctgtcgaa
      661 ccacatcacc gccaaccgcc gcaacggctt ctactcgatg gtagagcgca tccgcgccct
      721 ggccgcacgc gcggcctagc cgcctttccg attgacctaa atcaatgccg ccggcgcccg
      781 cgccgcgtag cgtgcccggc atgatcaagc ggctgcccta ctgagcatgc ccggcgcccg
      841 gctcgcgcca gcgcctgcgc gccgattcag cctgtggctg gcggcgctgc tgctgctgat
      901 cagcgcgcgc tcggtcgatg cacagggcga cgccatcgag atcatcagca gccccgagca
      961 tcgcggcgtc gagctcgacc gcaacacgct gcgcgccatc ttcaccatgc gcaagcgcga
     1021 atggcccgac ggcacgccgg tgcacgtgtt cgtgctgccc gacaccagcg acgtgcacga
     1081 ccagttcagc cgcgaagttc tcggcatcta tccctacgtg ctgcgcgaaa cctgggaccg
     1141 catggtctac accggcaccg gcttcgcgcc cgaaaccgtc aattcagaag ccgccatgcg
     1201 gcagaaagtc gaaaacacac ccggcgccat cggatacctg cgcaagcccc atggcagcgc
     1261 ggcggtggaa tccagaaagg acgtgcgcaa tgaacgctag gaagaaatcc ctgtaccgct
     1321 acatcggccc ggctgccgcg gcatgcgccg cgctggccgc cctgctgccc ggcgcagccg
     1381 cagccagctc gctggattac cagttgcatg gtttcgccgc gcagggcctg gtgctgagcg
     1441 acggcaacaa cgtgctgggc aacagcaccg gcggcagcgt ggcgttcaac gagttcggcc
     1501 tcaacggcgc cgtcaccctg
//
LOCUS       DASZZC010000117         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_845009,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000117 DASZZC010000000
VERSION     DASZZC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845009"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VHE37_02760"
     CDS             <1..683
                     /locus_tag="VHE37_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT34478.1"
                     /translation="NRDGTNVWPLDIVGIYAGNTKDVVFGASILVNYDYVDEGRSQSK
                     GTSSIYLVRVADPRSAGEIAQRIDKLFANSPYETKTMSEQQLVADSVKQIGDIGFVVN
                     SIVAAVFFALLFSVGAVMMQSIRERIPELAVLKTLGYSDAAVALLVGAESLLLCASAG
                     VLGLIAAWLALKPAAHAIASVLPLLRMTQETVIAGVVFALALGLVSAIVPVWQSARLT
                     IVAGLARR"
     gene            685..1866
                     /locus_tag="VHE37_02765"
     CDS             685..1866
                     /locus_tag="VHE37_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT34479.1"
                     /translation="MLRQLFAVSLMNLRSLPARWGTASVVVICLAGVAGVMVSMLAMA
                     EGFDSTFRRAGRADRVIVLTTGETYEAASSIARDQVTPLLSAPGLRQLPDGKPAAVIE
                     RLTMSALPLARSGRDGNLLLRGVSEKAWEVRPEVHIVAGRNFTPGLRELVVGRTAQSE
                     FTGLQLGGTVSLSGIGWTVVGIFASGGSAFESEAWADLEVVMDAYHQPSYSSITALLQ
                     SAAAFQQYKDAVTTNPQLNHSPIAEPDFYAAQGGTLATAMRVVGWLVAVIMSLGALFA
                     AINTLYASVESRTVEIATLRALGFGSAPVIGSVLLEAIVLCVIGAVTGGVLAYLLFNG
                     YTVSTLGGANFTQIQFAFAVTPALFAQGVLWSCGLGLIGGVPPAVSAARLPVAEALRG
                     G"
BASE COUNT          353 a          755 c          782 g          406 t
ORIGIN      
        1 ccaaccgcga cggcacgaac gtctggccgc tggacatcgt cggcatctac gccgggaaca
       61 ccaaggacgt cgtcttcggc gcctccatcc tggtgaacta cgactacgtc gacgaggggc
      121 ggagccagtc gaagggaacg agcagcatct accttgtccg ggtcgccgat ccgcgctcgg
      181 ccggcgagat cgctcagcgg atcgacaagc tcttcgccaa ttcgccctac gagaccaaga
      241 ccatgagcga gcagcagctc gtggccgatt ccgtcaagca gatcggcgac atcggcttcg
      301 tggtcaattc gatcgtggcg gcggtgttct tcgccctgct gttctcggtg ggcgcggtga
      361 tgatgcagtc gatccgcgag cgcatccccg aactggcggt gctgaagacg ctcggctaca
      421 gcgatgccgc ggtcgcgctg ctggtcggcg ccgaatcgct gctgctgtgc gcatcggccg
      481 gcgtgctggg gctgatcgcg gcgtggctgg cgctcaagcc ggcggctcac gccatcgcct
      541 cggtgctgcc gctgctgcgc atgacgcagg agacggtgat cgccggcgtg gtctttgccc
      601 tcgcgctcgg cctggtcagc gccatcgtcc cggtctggca atcggcgcgg ctgaccatcg
      661 tcgccggact ggcgcggcgc tgacatgctg cggcagctgt tcgcggtatc gctgatgaac
      721 ctgcgcagcc tgccggcgcg ctggggcacc gccagcgtgg tggtgatctg cctggccggc
      781 gtcgccggcg tgatggtgtc gatgctggcc atggccgagg gcttcgactc caccttccgc
      841 cgcgccggcc gcgccgaccg cgtgatcgtg ctcaccaccg gcgaaaccta cgaggcggcc
      901 agcagcatcg cgcgcgacca ggtgacgccg ctgctgtccg cgccgggcct gcggcagctc
      961 cccgacggca agccggccgc ggtgatcgag cggctgacca tgtcggcgct gccgctggcg
     1021 cgcagcggcc gcgacggcaa cctgctgctg cgcggcgtca gcgaaaaagc gtgggaggtg
     1081 cggccggagg tgcacattgt ggccggccgc aatttcacac ccggcctgcg cgaactggtg
     1141 gtgggccgca cggcacagag cgagttcacc ggcctgcagc tgggtggcac ggtgagcctt
     1201 tcgggcattg gatggaccgt ggtcggcatc tttgccagcg gcggcagcgc gttcgagtcc
     1261 gaggcctggg ccgatctcga agtcgtgatg gacgcctacc accagcccag ctacagctcg
     1321 atcaccgcgc tgctgcaatc ggccgccgcg ttccagcagt acaaggacgc cgtcaccacc
     1381 aacccgcagc tgaaccactc gccgatcgcc gagccagatt tctacgccgc ccagggcggc
     1441 acgctggcca ccgcgatgcg cgtggtcggc tggctggtgg cagtgatcat gtcgctgggc
     1501 gcgctgttcg ccgccatcaa cacgctgtac gcgtcggtgg aatcacgcac ggtggaaatc
     1561 gccacgctgc gcgcactggg gttcggcagc gcaccggtga tcggctcggt gctgctggag
     1621 gccatcgtgc tgtgcgtcat cggcgcggtg accggaggcg tgctggccta cctgctgttc
     1681 aacggctaca cggtgtcgac gctgggcggc gccaacttca cgcagatcca gttcgccttc
     1741 gccgtcacgc cggcgctgtt cgcgcagggc gtgctgtggt cctgtggcct cgggctgatc
     1801 ggcggcgtgc cgccggcggt gagcgcggca aggctgccgg tggccgaggc gctgcgcggc
     1861 ggttgactgc acgatcgctc ccgtggcgtt tgccgacgcg tatcccgcgc tcaagcatct
     1921 gcacctggtt tgcgccgcgg ccagcatctg caaacagcca taccgcaccc cgcctgggta
     1981 tgttattaat tatctattaa agtttaattt gataaataat ttataacatc aaaaaccatg
     2041 atgcggtcgg atgcgccagt ggttgatttg cccatcacgg ttgcagcggg cgttccggta
     2101 agctagaaga tgtaccccgc ccaaggatag ggcgcggtgt tccggctggg aggccacatg
     2161 aacatcagcg attccgctgt tgccgacgtc gaaatcgaaa gcgaagacgt tgcgtcgctg
     2221 gaccctcgcg tactgctcaa ggcactgcag gccatgaagg ccggcgattt ttccgtgcgc
     2281 ctgcccagcg accgca
//
LOCUS       DASZZC010000118         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_10818,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000118 DASZZC010000000
VERSION     DASZZC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10818"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VHE37_02770"
     CDS             <1..634
                     /locus_tag="VHE37_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT34480.1"
                     /translation="EVLLRMLDHAGDRILPGVFIAAAERYGLMPTVDRYVIEQVFARM
                     KQSARADDVVSINISGTSLSDDDFSDFIQRQFAEHALAPASVCFEITETAAIGNMGRA
                     LRFISEVRNLGCSIALDDFGSGMSSFNYLKSFHLDYVKIDGSFVRDMMNNPLDCATAE
                     AINKVAQVKGLKTVAEFVETAEALAKLKEMGVDYGQGYHLHAPEPWQQPG"
     gene            931..1761
                     /locus_tag="VHE37_02775"
     CDS             931..1761
                     /locus_tag="VHE37_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVT34481.1"
                     /translation="MPLEDSFYNAVATAVAQGTLTLPTLPDVAMKVDRMCREEDVSAA
                     ALATEIGKDPAIAVRLLRIANSAAFGNGARIDSLFQAIVRIGFSITRRLVSGLAMEQM
                     FNSSQPWLRALLKDSWTRSVEVAAHSQLLAAHCTELNPAVAMLGGLVHDVGVLPLIRL
                     AETQPQLQREHVVPVLRNAGPRTGWLVLRAWEFPPELVDVPAYCYDFERDHDGGPDYT
                     DVVSAAIVQQSCASGGEFRSQSRWNIPAFRKLGLDNPKALEEIEQKQAELAQARQVFA
                     "
     gene            complement(1768..2577)
                     /locus_tag="VHE37_02780"
     CDS             complement(1768..2577)
                     /locus_tag="VHE37_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVT34482.1"
                     /translation="MTDTIVQVAAVLGLYIALELALGRFPLRVSARNNLLDLAAFANA
                     TFLVGPLIVYGSALIERHLLPQYAGLWRAWPWWLQLAVLLLCEDHVQYWYHRATHRFA
                     WMWPAHKFHHSPEYMGVRIIWRNGFFYDLLMPNIWIAGVLVYLGFGQVYFWYYLVKLV
                     ITMGAHSELRWDEPLYRHRWLHPLAWIVERTISTPATHFAHHAYSEDDGIGHYNGNFG
                     NLLFFWDVLYGTARITRRYPPRFGVPDDAQSGPDPWQVYLFYPLFRRRPRG"
     gene            complement(2584..>3703)
                     /locus_tag="VHE37_02785"
     CDS             complement(2584..>3703)
                     /locus_tag="VHE37_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34483.1"
                     /translation="VDTSWTSSGDSLAGFGSANWHLLPRLDLTTGLRYTYEWKHAQVA
                     RTRYGGDPNASVLAAGDPFLDVLDAALGTNVEQYGFNGIIDDVAGGPYARADSLREGN
                     WSGQAALSYKLAPDVLSYASVARGYKGGGINLGFIGASIKPTFAPEQATSYEIGIKAR
                     LFERRLSLAFDIYQADIRNYQALTFDNEMTLLQNPRQINLLNVGQVRLRGAELEGYGY
                     AAPGLMLRAGVALSDAITTEFHSAPDEDNQQNDRDLSGQRLYDAPRWSGNLGAEYGYV
                     SDEPCEPYGAVDWSARTGYYGTVEHGRASYINGSRLLNLRAGLRARDHEWDVSLWMRN
                     ALNEKYVATVYPLYGVGDYGAVPGDPRTWGVTLRADLK"
BASE COUNT          627 a         1247 c         1232 g          597 t
ORIGIN      
        1 tgaggtgctg ctgcgcatgc tcgatcatgc cggcgaccgc atcctgcccg gcgtgttcat
       61 cgccgctgcc gaacgttatg gcctgatgcc gacggtggac cgttacgtga tcgagcaggt
      121 gttcgcgcgc atgaagcagt cggcgcgcgc cgacgacgtg gtcagcatca acatctccgg
      181 caccagcctg tccgacgacg atttctccga cttcatccag cgccagttcg ccgagcacgc
      241 actggcgccg gcctcggtgt gcttcgagat caccgaaacc gcggccatcg gcaacatggg
      301 ccgcgcgctc aggttcattt ccgaggtgcg caatctcggc tgcagcatcg cgctggacga
      361 cttcggcagc ggcatgtcgt cgttcaacta cctcaagtcg ttccacctcg actacgtgaa
      421 gatcgacggc tccttcgtgc gcgacatgat gaacaacccg ctggactgcg ccaccgccga
      481 ggcgatcaac aaggtggcgc aggtcaaggg cctgaagacg gtggccgagt tcgtcgagac
      541 cgccgaggcg ctggccaagc tcaaggaaat gggcgtcgac tacggccagg gttatcacct
      601 gcacgcgccg gaaccctggc agcagcctgg ctgaatccag cctgaacggc gcagcgttaa
      661 caaacgcaaa gttggttgtg ctgcggcagg cgtgatgcaa aatgatcgcg tcaccggcat
      721 ccccgacatc taccccgatg ttgctggtga cgacgcacag cattgccccc aatgctggcc
      781 ccgtacggcc ggcgcatctc cccggatgcg ccggccattt attttccgcc tcccatcatc
      841 cagcaccgtc cgcgcgtctg ccgcgggcca gcgcggcgca tgcgcggctg ttagcgtgct
      901 ccgcccgacc cttcgcccga gccaagcccg atgccgctgg aagacagctt ctataacgcc
      961 gtcgccactg ccgtcgcgca gggcaccctg accctgccga ccctgcccga cgtggcgatg
     1021 aaggtcgacc gcatgtgccg cgaggaagat gtcagcgctg ctgccctggc cacggagatc
     1081 ggcaaggacc cggccatcgc cgtgcgcctg ctgcgcatcg ccaacagcgc cgccttcggc
     1141 aacggcgcac gcatcgacag cctgttccag gccatcgtgc gcatcggctt ctccatcacc
     1201 cgccggctgg tcagcggcct ggccatggag cagatgttca actcgtcgca gccctggctg
     1261 cgggcgctgc tcaaggacag ctggacgcgc agcgtcgagg tggccgcgca cagccagctg
     1321 cttgccgcgc actgcaccga actcaatccc gcggtggcca tgctgggcgg gctggtgcac
     1381 gacgtcggcg tgctgccgct gatccgcctg gcggaaacgc agccgcagct gcagcgcgag
     1441 cacgtggtgc cggtgctgcg caatgccggg ccgcgcaccg gctggctggt gctgcgcgcc
     1501 tgggaatttc cgccggagct ggtcgatgtg ccggcctact gctacgactt cgagcgcgac
     1561 cacgatggcg gtccggacta taccgacgtg gtcagcgcgg ccatcgtgca gcagagctgc
     1621 gccagcggcg gcgagttcag gagccagtcg cgctggaaca ttcccgcctt ccgcaagctc
     1681 ggcctggaca acccaaaggc cctcgaggag atcgagcaga agcaggccga actggcgcag
     1741 gcgcggcagg tgtttgcctg aaacatctca gccgcgcggc cggcggcgga atagcgggta
     1801 gaacaggtag acctgccagg gatcagggcc ggactgcgca tcgtcgggca caccgaagcg
     1861 cggcgggtag cgtcgcgtga tgcgcgcggt gccgtagagc acatcccaga agaacagcag
     1921 gttgccgaaa ttgccgttgt agtggccgat gccgtcgtcc tcgctgtagg cgtggtgcgc
     1981 gaagtgcgtg gccggcgtgg aaatggtgcg ctcgacgatc cacgccagcg ggtgcagcca
     2041 gcggtggcgg tacagcggct cgtcccagcg cagttcgctg tgcgcgccca tggtgatgac
     2101 cagcttgacc aggtagtacc agaaatacac ctggccgaaa cccaggtaca ccagcacgcc
     2161 ggcgatccag atgttcggca tcagcaggtc gtagaagaac ccgttgcgcc agatgatgcg
     2221 cacacccatg tactccggcg agtgatgaaa cttgtgcgcc ggccacatcc atgcaaagcg
     2281 gtgggtggcg cggtgatacc agtactgcac gtggtcctcg cacagcagca gcaccgccag
     2341 ctgcagccac cacggccagg cgcgccacag gccggcgtac tgcgggagca agtgacgttc
     2401 gatcagcgcg ctgccgtaga cgatcagcgg gccgaccaga aaggtggcgt tggcgaaggc
     2461 cgcgaggtcg agcaggttgt tgcgcgccga cacgcgcagc gggaagcgcc cgagcgcgag
     2521 ctccagcgca atgtacaggc cgagcaccgc ggccacctgg acgatggtgt cagtcatgcg
     2581 cgctcacttc aggtcggccc gcagggtcac gccccaggtg cgcggatcgc ccggcaccgc
     2641 gccgtagtcg ccgacgccgt agagcggata caccgtcgcc acatattttt cgttgagcgc
     2701 attgcgcatc catagcgaga catcccattc gtgatcgcgc gcgcgcaggc cggcgcgcag
     2761 gttgagcagc ctggaaccgt tgatgtagct ggcgcggccg tgctccaccg tgccgtagta
     2821 cccggtcctg gccgaccagt ccactgcccc gtatggttcg cacggctcat ccgacacgta
     2881 accgtattcc gcgcccaggt tgccgctcca gcgtggcgcg tcgtacaggc gctggccgga
     2941 gagatcgcgg tcgttctgct ggttgtcctc gtccggagcg ctgtggaact cggtggtgat
     3001 ggcgtcggac agcgccacgc cggcgcgcag catcagcccc ggcgcggcgt agccgtagcc
     3061 ttccagttcg gcgccgcgca gccgcacctg gccgacgttg agcaggttga tctgccgcgg
     3121 gttctgcagc agcgtcatct cgttgtcgaa ggtcagcgcc tggtagttgc ggatgtcggc
     3181 ctggtagatg tcgaaggcca gcgacaggcg ccgctcgaac agccgcgcct tgatgccgat
     3241 ctcgtacgag gtggcctgct ccggtgcgaa agtcggcttg atcgacgcgc cgatgaatcc
     3301 caggttgatg ccgccgccct tgtagccgcg cgccaccgac gcgtaggaca gcacgtccgg
     3361 cgccagtttg tacgacagcg cggcctgccc cgaccagttg ccttcgcgca gcgagtcggc
     3421 gcgcgcgtac gggccgccgg ccacgtcgtc gatgatgccg ttgaaaccgt actgctcgac
     3481 gttggtgccc agcgcggcgt cgagtacgtc gaggaacgga tcgcccgccg ccagcacgct
     3541 ggcattcgga tcgccgccgt agcgcgtgcg cgccacctgc gcgtgcttcc attcgtaggt
     3601 gtagcgcagc ccggtggtca ggtcgagccg cggcagcagg tgccagttgg ccgaaccgaa
     3661 acccgccagg ctgtcgccgc tcgaggtcca cgaggtatcg acg
//
LOCUS       DASZZC010000119         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_650189,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000119 DASZZC010000000
VERSION     DASZZC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650189"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VHE37_02790"
     CDS             complement(<1..631)
                     /locus_tag="VHE37_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HVT34484.1"
                     /translation="MNADAALAPAKHDPFAGPPLSGRVPLTESQREIWLAAQMHSEAA
                     VAYNEGLAISLHGELDVAALGAAVDQLVQRQPLLRAGVSPNGRWISVGEPRGGSLQHT
                     PGDLESAEREEMARPFDLEQGPLMRFRLVTLAPQEHVLLMIAHHVMVDGWSYATLLQE
                     LGRLYTAQVEGGAAPLPEVQPFADYVEAERRFVESAEGQRHADYWLKQLE"
     gene            complement(641..5407)
                     /locus_tag="VHE37_02795"
     CDS             complement(641..5407)
                     /locus_tag="VHE37_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HVT34485.1"
                     /translation="MAHTLRARGVVRGSAVALLLPRGIELIVAMLAVQKAGGYFVPVD
                     PVLPDARIDYLLQDSAPVVAISSSTVGASERIASEKRLLLDVDRLAEDVAAPSVELTP
                     DDAVYAIYTSGSTGKPKGALNFQKGVANLYTWFAQELELDAQTRSLVVGSLSFDLYHK
                     GVFAPLLSGGCVVLYASAAFDAAEIRALVASEGVTLICATPSAFNGIIEHAGERGMAQ
                     LASLRRVSLGGEVVQKARLRPWLSHPSCAARVINTYGPTECADIQSYHWITADEMRHD
                     QPVPIGRALRGCELYALDADMKPVAAGEVGRLWLGGTCVGGGYLNLPDRTAQAFLPNP
                     FRAGERMYDTGDLVSWNAAGELDYRGRADRQVKIRGYRIELDEVEAQLAALPGVIECA
                     ATAPVGPADERVLRAYVRMEPGVVADISSLRRQLAERVPDYLVPSVWVMLERFPYNNS
                     GKIDRNALPEPAPSAAAASASPQLTLEQRIAERWRELIGLDRFDHDTPFIELGGTSLL
                     AVRFVGQLAGELDVAIPMVDFFAASTVNRFSTYLRQRHAAAVSRWTGENVERVQQHQR
                     QLRRTATSGGAVAVVGMACRVPGADDVEVLWQHLLEGRDTLQRDPEWAKDRGADWVSA
                     AGWLDHADCFDHEFFRYTPREAAITDPQQRILLECAWHALEHAGIAPDRSDMAIGVYA
                     GLAANSYLTRNLASHAEFREFGMDYASIGNDKDFAATRIAYKLDLRGPAMTIQTGCSS
                     SGTALHMACLALQAGDCDAAIVGGSSLPWRYRHGHRHVEDGPLSRDGYIRSFDARASG
                     MVLTGGVACVVLKRLADAQADGDTIHSVIRATALNNDGSNKAAFTAPNVEQQTAVIRR
                     ALQRAGVSADDISCVEAHGTGTPLGDPIEIAGLTRAYREDSQRTGYCAIGSVKGNIGH
                     LDAGAACAGLIKLSLALTHRKLPRQPHFEQPHPECRMEQTPFQVHRETVEWRAAFPRR
                     AGLSSFGFGGTNFHAVIEEAPAAVASSTSSRKWHVLRLSAKNGDALARQAGRMAAWRV
                     SHPDAPLADAAWTLDVGRARLAERGALVVSDAVDPARLIRGKAVRSPASLVFVFPGQG
                     AQHPGMGRQLYRDEPVFRQAVDRCAEVLGKPLGFDLRELLFSSDSTAAERLRDTSLAQ
                     PAIYTISYALAQTWMSWGYQPGALAGHSVGEFVAATLAGVFTLEDALAVLAERAALMR
                     SMPAGSMLAVRLGEDELRAQLPDGLDLAAVNASQLCVVSGPAEAVAAFADELRARGVA
                     TTPLHTSHAFHSSMMDPVVQRFEQVVQRHPRGKLQLPLMSTVTARWLLDSEATDPAYW
                     SRQLRHTVRFAPAMAELLKTPGRVLLEVGPGQSLAAAMRQMAGPACDAAVVASMPHAQ
                     SADADDHEHAVQAMARLWLAGIEADPARYYAGERRNRIGLPGYPFARVRHWIDPARTF
                     DTADATAPAPATEIVAAAGAAAAPPTIEQQVLAALLARSGIEVTPADADRTFLEIGFD
                     SLLLTQLAADLKQRFSVDLRFRQLLRELPTPRALIAHLQQRVPEAGVRQAADDASESS
                     VPVARGQARLGRDAEGNPAWFVPDPEHEGAFLQLVE"
BASE COUNT          890 a         1892 c         1805 g          827 t
ORIGIN      
        1 cttcgagctg cttcagccaa tagtcggcgt ggcgctggcc ttccgccgat tcgacaaacc
       61 tgcgctcggc ctctacgtaa tcggcgaacg gttgcacttc cggcaacggc gccgcgccgc
      121 cttccacctg cgccgtatac agccggccca gctcctgcag cagcgtggca taggaccagc
      181 catccaccat tacgtggtgc gcaatcatca gcagcacgtg ttcctgcggc gccagcgtca
      241 ccaggcggaa gcgcatcagc ggtccctgct ccaggtcgaa cggccgcgcc atctcctcgc
      301 gctcggcact ttccagatcg cccggcgtgt gctgcagcga gccgccgcgg ggctcgccca
      361 cgctgatcca gcgcccattc gggctgacgc ccgcgcgcag cagcggctgg cgctgcacca
      421 gctggtcgac cgccgcaccg agcgcggcaa cgtcgagttc accgtgcagg ctgatggcca
      481 ggccttcgtt gtaggccacg gccgcctcgc tgtgcatctg cgcagccagc cagatttcgc
      541 gttgcgattc ggtcagcggc acgcgcccgg ataacggcgg cccggcgaag ggatcgtgct
      601 tcgcgggggc gagcgcggca tcagcgttca tcggacactc tcattcaacc agttgcagga
      661 aagcaccttc gtgctcggga tcgggcacaa accacgcggg gttgccctcg gcatcgcggc
      721 ccagtcgcgc ctgcccgcgc gcgaccggca ccgaagactc agacgcatcg tcagctgcct
      781 ggcggacacc ggcctccggc acccgctgct gcagatgtgc aatcagcgca cgcggggtcg
      841 gcagctcgcg cagcagctgg cggaagcgca ggtcgacgct gaagcgctgc ttgagatcgg
      901 ccgccagttg cgtcagcagc agggagtcga agccgatctc caggaacgtt cgatcagcat
      961 ccgccggcgt gacctcgatc ccgctgcgcg ccagcagcgc cgccagcacc tgctgctcga
     1021 tggtcggcgg agcggcggcc gcgccggctg ctgctacgat ttccgtagca ggcgctggcg
     1081 ccgttgcatc tgccgtatcg aacgtcctgg ccgggtcgat ccagtggcgc acccgcgcaa
     1141 acgggtatcc aggcaggccg atgcgattgc gccgctcacc ggcgtaataa cgcgcggggt
     1201 ccgcttcgat gccggccagc cacaggcgtg ccatggcctg caccgcatgt tcatggtcat
     1261 ccgcgtccgc actctgcgcg tgcggcatcg aagccaccac tgcggcatcg caggcaggcc
     1321 ctgccatctg ccgcatcgcc gccgccaggc tctggcccgg ccccacttcc agcaacacgc
     1381 gcccgggtgt ctttagcagc tcggccatcg ccggcgcgaa acgcacggtg tgacgcagct
     1441 ggcgcgacca atacgcggga tcggtcgcct cgctgtcgag cagccagcgc gcggtgacgg
     1501 tggacatcag cggcagttgc agcttcccgc gcggatgccg ctgcaccacc tgctcgaagc
     1561 gctgcaccac cgggtccatc atcgacgaat ggaaggcatg cgaggtatgc agcggcgtgg
     1621 tcgccacgcc gcgcgcgcgc agctcatcgg caaaggcggc gacagcttct gccgggccgg
     1681 acaccacgca tagctgggaa gcattgaccg cagccaggtc caggccgtcc ggcaactgtg
     1741 cgcgcaattc gtcttcgccc aggcgcacag ccagcatgct gcctgccggc atgctgcgca
     1801 tgagcgcggc gcgttcggcc agtaccgcca gcgcgtcttc cagcgtgaac acaccggcca
     1861 gtgtcgctgc cacgaattcg cccacgctgt gaccggccag ggcgcccggc tggtagcccc
     1921 agctcatcca ggtctgcgcc agcgcgtagc tgatggtgta aatggccggc tgcgccagcg
     1981 aggtgtcgcg cagacgctcc gccgcagtgg agtcgctgga aaacagcagt tcgcgcaggt
     2041 cgaaccccag cggcttaccc agcacttcag cgcagcgatc gaccgcctgg cgaaacaccg
     2101 gttcgtcgcg gtacagctgc cggcccatgc cgggatgctg cgcgccctgt ccgggaaaca
     2161 cgaacaccag gctcgccgga gagcgcaccg ccttgccgcg gatcagccgc gccggatcga
     2221 ccgcatcact caccaccagc gcgccgcgtt cggccagccg cgcacgcccg acatccagcg
     2281 tccacgcggc atcggccagc ggcgcatccg ggtgagacac tcgccacgct gccatccgcc
     2341 cggcctggcg cgcgagcgca tcgccgttct tcgccgacaa tcgcagcacg tgccacttgc
     2401 gcgacgacgt gctggaagcc actgcggctg gcgcttcttc gatcacggca tggaagttgg
     2461 taccgccgaa gccgaacgaa ctcaggccgg cccggcgcgg aaatgccgcg cgccattcca
     2521 ccgtttcgcg gtgcacctgg aacggcgtct gctccatgcg gcattcggga tgcggctgct
     2581 cgaagtgcgg ctgcctcggc agcttgcggt gcgtcagcgc gagcgacagc ttgatcaggc
     2641 cggcgcatgc cgcgccggca tcgaggtggc cgatgttgcc cttgacgctg ccgatggcgc
     2701 agtagccggt gcgctggctg tcctcccggt aggcgcgcgt gagcccggca atctcgatcg
     2761 gatcgcccag cggtgtgccg gtgccatgcg cctcgacgca gctgatgtca tcggcggaaa
     2821 cgccggcacg ctgcagcgcg cgccggatca ccgcggtctg ctgctccacg ttgggcgcgg
     2881 tgaacgcggc cttgttgctg ccgtcgttgt tgagcgccgt ggcacggatc accgaatgga
     2941 tggtgtcgcc atcggcctgc gcatcggcca ggcgcttgag caccacgcag gcgacacctc
     3001 cggtaagcac catgccgctg gccctagcat cgaacgagcg gatatagccg tcgcggctga
     3061 gcggcccatc ctcaacgtgg cgatggccgt ggcgataacg ccagggcagc gaggagccgc
     3121 cgacgatggc cgcatcgcaa tcgcccgcct gcagggcgag gcaggccatg tgcagcgcgg
     3181 tgccgctgga cgagcagccg gtctggatgg tcatggccgg cccgcgcagg tcgagcttgt
     3241 aggcaatgcg cgtggcggcg aagtccttgt cgttgccgat gctggcgtag tccatgccga
     3301 actcgcggaa ctcggcgtgg ctggccaggt tgcgcgtgag gtagctgttc gccgcgaggc
     3361 cagcgtaaac gccgatggcc atgtcgctgc ggtcgggcgc gatgccggca tgctccagcg
     3421 catgccaggc acattcgagc aggatgcgct gctgtggatc ggtgatggcc gcttcgcgcg
     3481 gcgtgtaacg gaagaactcg tggtcgaagc aatccgcgtg atcgagccaa ccagctgcgg
     3541 aaacccagtc cgcgccgcgg tccttcgccc actccgggtc gcgttgcaag gtgtcgcgcc
     3601 cttcgagcag gtgctgccac agcacctcga catcgtctgc tccgggaacc cggcaggcca
     3661 tgccgaccac ggcaaccgcc ccccccgagg tcgccgtgcg ccggagctgc cgctggtgct
     3721 gctgcacccg ctcgacgttt tcgccggtcc agcgcgaaac cgccgcggcg tggcgctggc
     3781 gcaggtaggt gctgaagcgg ttgacggtgg acgcggcgaa gaaatcaacc atcgggatgg
     3841 ccacgtcgag ctcgccggca agctgcccga cgaagcgcac cgccagcagg ctggtgccgc
     3901 ccagttcaat gaaaggcgtg tcgtggtcga agcgatcgag tccgatcagc tcgcgccacc
     3961 gctcggcgat gcgctgttcc agcgtcagtt gcggcgacgc ggatgcagcg gcggcggacg
     4021 gcgccggttc aggcaaggca ttgcggtcga tcttgcccga gttgttgtac gggaaacgtt
     4081 cgagcatcac ccaaaccgac ggcaccaggt aatcgggcac ccgttcggcc agctgccgcc
     4141 gcagcgagga aatgtcggcc acaacgccgg gctccatgcg cacgtaggca cgcagcaccc
     4201 gctcgtccgc cgggcccacg ggagcagtgg ctgcgcactc gatgacgccc ggcagggcgg
     4261 ccagttgcgc ttccacttcg tcgagctcga tacggtatcc gcggatcttc acctggcgat
     4321 ccgcgcggcc gcggtagtca agctcgccgg cagcgttcca gctgaccagg tcgccggtgt
     4381 catacatgcg ctcgcccgcg cggaatggat tgggcaggaa ggcctgcgcg gtgcgatccg
     4441 gcaggttcag ataaccgccg ccgacgcagg ttccgccaag ccataggcgg ccgacttcgc
     4501 cggcagcaac aggtttcata tcggcatcca gcgcgtagag ctcgcagccg cgcaaggcac
     4561 gaccgatcgg caccggctgg tcatggcgca tctcgtcagc ggtgatccag tgatagctct
     4621 ggatgtcggc gcattcggtc gggccgtagg tgttgatcac gcgcgctgcg caggaaggat
     4681 gcgacagcca cggccgcaga cgcgccttct gcaccacttc gccgcccagc gaaacgcgtc
     4741 gcagactcgc cagctgcgcc atgccgcgct cgcccgcatg ttcgatgatg ccgttgaagg
     4801 cgctgggcgt ggcgcagatc agagtgacgc cttcgctggc caccagtgcg cggatttccg
     4861 ccgcatcaaa agcggcggag gcatacagca ccacgcagcc gccggacagc agcggcgcaa
     4921 acacgccctt gtggtacaga tcgaacgaca gcgagccgac gaccagcgag cgcgtctgcg
     4981 catccagttc cagctcctgc gcaaaccagg tgtagaggtt ggccacaccc ttctggaagt
     5041 tgagcgcacc tttaggcttg ccggtggaac cggaggtata gatggcatat accgcgtcgt
     5101 ccggcgtcag ctcgacggac ggcgctgcca cgtcttcggc caggcgatcc acgtcgagca
     5161 acagacgttt ttccgatgca atgcgctcgc tggcacccac ggtgcttgaa ctgatcgcca
     5221 cgaccggcgc ggaatcctgc aggagatagt cgatgcgggc atccggcagc accgggtcga
     5281 ccggcacgaa atagccgccg gccttctgca cggcgagcat cgccacgatc agctcgatgc
     5341 cgcgcggcag cagcagagcc acggcactgc cgcggaccac accgcgcgcg cgcagtgtgt
     5401 gcgcgatgcg attg
//
LOCUS       DASZZC010000120         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_650201,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000120 DASZZC010000000
VERSION     DASZZC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650201"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2538
                     /locus_tag="VHE37_02800"
     CDS             <1..>2538
                     /locus_tag="VHE37_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34486.1"
                     /translation="DMLIRRVAQILSADGSSIAYDHIFNHVTHNHSAAYYSTLAVGVW
                     LFEDVYDQRLFEYQARAIAHAIEMAEGNLKPARMGATTIKHYLYKGNVVRQANADDGT
                     PAGYPMEYNKHDLVVMRFDDLSDPAHPKPLAAWINWGEHPESLDGYNLHSGDYIAPLQ
                     RFVDSDIGAPMVFSQGDVGSSENTANPCQVLDPQGHLIEEAIAHDASGKPLYGGNGSP
                     QCTPTPKQSDDVGVLRDWDHQGYVQTERNVRYLADDIKRAWDAIGAGNARVPYSSDFP
                     VHSYLRWTPGPLSHPYPSVSNCRTDPTLNGDVGVPAAGLPDCERASDEGVPTQAVAPA
                     LEPLALVIETLKEHGLGVVPDNYDAPAFSVVEENLRLKLHTVSLGEVLLASCACEAQN
                     DLILNLESRTDKPQHNIYDGFDWACLLPQYKDDAHYKAACDLQNGTYYDPALQQAPPP
                     GTNFDAAAIAHMRAEVHNDAAGWDAPANALTAGSEPADISKIWGNFTKEELDAAHGYL
                     LPVGVGHGGDYNGYTVSFREYQNRDSYRKALTSYGPHTADYMVTRLVRMAGAMRDGST
                     IPDEPDDVLAQADELRQQALATALGNLTGPAYDLFLKALPPDLGPAAAVTQPKNITHF
                     DAATFSWTGGNNQVDNPLVTVERQNTDHSWSAVYRQNGFVQTMVTYPEGLSGAAMTYI
                     GNTRWKWTASFEAYAAFPSTLESIAPGTYRFAVQGHIINTRGTIDDYALNSDPFTVSP
                     WSGIVVKNAAVNGGDISFDADPILYPQTYTSPFPFIDGTDQDQRICKQCTFRPWAHVG
                     HVASVQVTRAGGTVELVPASGSGGHYTAAAQLAAGDSRVIRVIDDHN"
BASE COUNT          487 a          916 c          792 g          343 t
ORIGIN      
        1 aggacatgct gatccgccgc gtggcgcaga tcctgtcggc cgatggctcg tcgatcgcct
       61 acgatcacat cttcaaccac gtcacccaca accacagcgc cgcgtactac tcgaccctgg
      121 cggtgggcgt atggctgttc gaggacgtgt acgaccagcg cctgttcgag taccaggcac
      181 gcgccatcgc ccacgccatc gagatggcgg agggcaacct caagcccgca cgcatgggcg
      241 ccaccaccat caagcactac ctgtacaagg gcaacgtggt gcgccaggcc aatgccgacg
      301 acggcacgcc ggccggctac ccgatggaat acaacaagca cgatctggtg gtgatgcgct
      361 tcgacgacct cagcgacccc gcccatccga aaccgctggc agcgtggatc aactggggcg
      421 agcatccgga atcgctcgac ggctacaacc tgcacagcgg cgactacatt gcgccgctgc
      481 agcgcttcgt cgactccgac atcggcgcgc cgatggtgtt ttcgcagggc gatgtcggca
      541 gcagcgagaa caccgccaat ccctgccagg tgctggaccc gcagggtcac ctgatcgagg
      601 aagccatcgc ccacgacgcc agcggcaagc cgctgtacgg cggcaatggc tcgccgcaat
      661 gcacgcccac gcccaagcag tccgacgacg tcggcgtgct gcgcgactgg gatcaccagg
      721 gctacgtgca gaccgagcgc aacgtgcgct acctggccga tgacatcaag cgcgcctggg
      781 atgcgatcgg cgcgggcaac gcgcgcgtgc cgtacagcag cgatttcccg gtgcactcgt
      841 acctgcgctg gacgccgggc ccgctgtcgc acccgtatcc gagcgtgtcc aactgccgca
      901 ccgatcccac gctcaatggc gatgtcggcg tgccggccgc cggactgccg gactgcgaac
      961 gcgccagcga cgaaggcgtg ccgacgcagg ccgtcgcgcc ggcgctggaa ccgctggcgc
     1021 tggtgatcga aacgctcaag gagcacggcc tgggcgtggt gcccgacaac tacgatgcgc
     1081 cggcgttcag cgtggtggag gagaacctgc gcctcaagct gcacacggtg agcctgggcg
     1141 aagtgctgct ggcctcctgc gcctgcgagg cgcagaacga cctgatcctc aacctcgagt
     1201 cgcgcaccga caagccgcag cacaacatct acgacggctt cgactgggcc tgcctgcttc
     1261 cgcaatacaa ggacgacgcc cactacaagg cggcctgcga cctgcagaac ggcacgtact
     1321 acgacccggc gctgcagcaa gcgccgccgc cgggcaccaa cttcgatgcc gccgccatcg
     1381 cgcacatgcg cgcggaagtg cacaacgacg ccgccggctg ggacgcgccg gccaacgcgc
     1441 tcaccgccgg cagcgagccc gcggacattt ccaagatctg gggcaacttc accaaggaag
     1501 aactcgacgc cgcgcacggc tacctgctgc cggtgggcgt gggccatggc ggcgactaca
     1561 acggctacac cgtgagcttc cgcgaatacc agaaccgcga cagctaccgc aaggcgctta
     1621 cctcctacgg accgcacacc gccgactaca tggtcacgcg gctggtgcgc atggccggcg
     1681 ccatgcgcga cggcagcacc attcccgacg agccggacga cgtgctggcg caagccgacg
     1741 agctgcgcca gcaggcgctg gccaccgcgc tgggcaatct caccggcccg gcctacgacc
     1801 tgttcctcaa ggcgctgccg cccgacctcg gcccggccgc ggccgtgacc cagcccaaga
     1861 acatcaccca cttcgatgcc gccaccttca gctggaccgg cggcaacaac caggtcgaca
     1921 acccgctggt gacggttgag cggcagaaca ccgaccacag ctggagcgcg gtctacaggc
     1981 agaacggctt cgtgcagacc atggtgacgt atccggaagg cctgtccggc gcggccatga
     2041 cctacatcgg caacacccgc tggaaatgga cggccagctt cgaagcctac gccgccttcc
     2101 ccagcacgct ggaaagcatt gcgccgggca cctaccgctt tgcggtgcag gggcacatca
     2161 tcaacacgcg cggcaccatc gacgattacg cgctcaattc cgatccgttc accgtctcgc
     2221 cgtggagcgg catcgtggtg aagaacgcgg ccgtgaacgg cggcgacatc agcttcgacg
     2281 ccgatccgat cctctatccg cagacctaca cctcgccgtt cccgttcatc gacggcaccg
     2341 accaggacca gcgcatctgc aagcaatgca ccttccggcc gtgggcgcat gtcggccatg
     2401 tcgccagcgt gcaggtgacg cgcgccggtg gcacggtgga attggtgccg gcgagcggca
     2461 gcggcggaca ctacacggcg gccgcgcagc tggctgcggg cgacagccgc gtcatccgcg
     2521 tgatcgacga tcacaacg
//
LOCUS       DASZZC010000121        13751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_267622,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000121 DASZZC010000000
VERSION     DASZZC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 13751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13751
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267622"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1030)
                     /locus_tag="VHE37_02805"
     CDS             complement(143..1030)
                     /locus_tag="VHE37_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34487.1"
                     /translation="MSSNHIAHRYAEVNGVRLHYAAAGHGKLMLFIHGWPEFWYAWKE
                     QLAEFSRDHFVVAPDTRGVNLSSKPQDVSAYHARELVEDMRQLIEQLGHGKAIVVAHD
                     WGGAIAWNLAAQHPEMVEKLVILNSPHPMPYMRELARNPAQQAASAYMLLLADAKAER
                     VLMQDDYRRLRAQLDGWKKSARPPTQEDLDLYMQAWTQPGALTAGLNYYRASPLRPPS
                     ETSANAAGLKLDGAQFTVRVPTLVIWGMQDSALTPALLDGLEQWVPDLRIERIEQATH
                     WVAHEFPERVNALIRGFVT"
     gene            complement(1027..2172)
                     /locus_tag="VHE37_02810"
     CDS             complement(1027..2172)
                     /locus_tag="VHE37_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT34488.1"
                     /translation="MNFEFSEEQTALRDTLLRYLQKNYGFEQRREIIRSTDGWSRAHW
                     KQFAEMGLTAIGLPEAYGGYGLGGPFDTYVVMEAFGRALVVEPYLASVVLAADLIAHV
                     GSGAQKSALLPKVASGELQLALAHQEPGARYELAHVATKSVASGNGYVLDGAKTVVLN
                     GAAADRLIVSARTAGGERDEAGISLFLVDPKSSGVSIHGYPTQDGQRAADITLKGVQV
                     SADALLGQAGAALPAIEHQLQHAIAALCAEAVGAMGALLEITGAYLKTRKQFGVPIGS
                     FQALQHRMADMLMQLESARSMAYLIAARIGSADLQERRRAVAAAKALVGQAARHVGQQ
                     AVQLHGGIGVTDELNVSHYFKRLTMIDLTFGDAAHHLARYSDLMLAA"
     gene            complement(2176..3375)
                     /locus_tag="VHE37_02815"
     CDS             complement(2176..3375)
                     /locus_tag="VHE37_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT34489.1"
                     /translation="MDLNFTAEDERFRAEVREFVAHNLKPAIRDKVLHGKRITREDFL
                     DWHKTLHRKGWVASTWPKRFGGTGWNATQQHIFEEECALGGAPALMPFGLRMVAPVIM
                     AFGSQAQQEHFLPRIRSGEDWWCQGYSEPGSGSDLASLKTRAVRQGDKYIVNGQKTWT
                     TLAQFADWIFCLVRTNPEVRHQEGISFLLIDMKSRGITVRPIIMLDGEHEINEVWFEN
                     VEVPAANLVGEENKGWTYAKYLLGHERTGNASIGMCKRWLNHLKEIVRRQPADGGRSL
                     MQDPRFRDRVAQVEINLMALEITNLRVIEMEQQRKAPGPEASMLKVKGVEIQQALTEL
                     MMLALGPQALAYQRAALDADYRGPLAGPEYGPPLPGHYFNFRKASIYAGSDEIQRNII
                     ARMMLGL"
     gene            3491..4051
                     /locus_tag="VHE37_02820"
     CDS             3491..4051
                     /locus_tag="VHE37_02820"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HVT34490.1"
                     /translation="MKLTKAYRNAEFLSSEDARSIRILAEYLEPASRFRRKNIRKAII
                     FFGSARINKLTRRTSRYYREAVALAEKLARWTTQVHAKPERYHICTGGGPGIMEAVHE
                     GAAKVDRKLNIGLNISLPFEQHVNPHVIKGHALEFHYFFMRKFWFLNVAHAAVIFPGG
                     FGTLDELFELLTLTQTGKSSPMPIEL"
     gene            4290..4418
                     /locus_tag="VHE37_02825"
     CDS             4290..4418
                     /locus_tag="VHE37_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HVT34491.1"
                     /translation="MLRTSADVERCSTQGGKMGFDPLLLLALLGFRRKRRRKPATG"
     gene            complement(4457..4699)
                     /locus_tag="VHE37_02830"
     CDS             complement(4457..4699)
                     /locus_tag="VHE37_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34492.1"
                     /translation="MPRKPKKPQPQPDAAKNLDEFGDESDVAEEEVAEVEEHAAEKMR
                     DWRDVEKYKEELSLRRLIDDDLDIDDLPTSRHPRQK"
     gene            4915..4990
                     /locus_tag="VHE37_02835"
     tRNA            4915..4990
                     /locus_tag="VHE37_02835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4948..4950,aa:Ala,seq:cgc)
     gene            5060..6286
                     /locus_tag="VHE37_02840"
     CDS             5060..6286
                     /locus_tag="VHE37_02840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HVT34493.1"
                     /translation="MASVTRQTFNEVMVPNYQPADIIPVRGAGSRVWDQQGREYIDFA
                     CGIAVTGLGHCHPRIVAALTEQANKLWHLSNVMTNEPALRLAKKLTELTFAQKVFFCN
                     SGGEANEAAFKLARRYGNTQFGPHKHQIISFFNAFHGRTLFTVSVGGQAKYTAGFEPI
                     PGGIIHLPYNDIAALEAAVGEQTCAVVVELVQGEGGITPADPAFIKRARELCNEHKAL
                     LIFDEVQTGNGRCGPLYCYQHYGVTPDVLTTAKGLGAGFPIGAMLTTDDCAKVLAFGT
                     HGSTYGGNALGCAVAEAVLDELSKPELVANASARATQLRDGIAAIGKRYGIFDGSRGL
                     GLLIGAPMSAAWKGKAKEVVNAGLRHGLWCLIAGPDVLRLAPALNIGEAEVAEGLKRL
                     DAACAELAALPKVSNG"
     gene            6356..6832
                     /locus_tag="VHE37_02845"
     CDS             6356..6832
                     /locus_tag="VHE37_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34494.1"
                     /translation="MSDRRLLLLAAALLTGCAAGYVEPDVSPTVAYLTLEPGDMNGAT
                     RIVYSAFHDAACTPREGDGAVATVGLGAGPAKTIRVFSGGPIYIRALRQITVANGTLR
                     SCVNLISFVPQPDRQYRLMPETDADRPCELTLIDAHTEKNEASLKRLQPGLACSAP"
     gene            complement(7038..7787)
                     /locus_tag="VHE37_02850"
     CDS             complement(7038..7787)
                     /locus_tag="VHE37_02850"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HVT34495.1"
                     /translation="MSLLLDALRRAEQERNAVQNGLQPPPAVSAPEARVLPWPLIAVC
                     VLLAFACGALLIALLYKPRAAAAPAVVAAVPAPAPVDAAAPPAPAEESEVIPPQAAQT
                     INSLDDLADSNSASNAPAEPSAPLPAEVTAPAQPASAPAGDEVAADDAQAEAPPPKLE
                     VEHLKLAPAPPKQIPELKDMPPDFRANFPRLTLDVHVYDDDPAKRFVMISGHRYREGD
                     ALREGPQVEQIAEGGVVLRWSGRQVLLPIPH"
     gene            complement(7784..9433)
                     /locus_tag="VHE37_02855"
     CDS             complement(7784..9433)
                     /locus_tag="VHE37_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT34496.1"
                     /translation="MTTLAAAVYLEHFHLREMPFSITPDPAYLYLSPRHQEALGHLLY
                     GTGQYGGFVQLTGEVGTGKTTVVRALLTQKLAAVDVAMIHNPRQSEQEFVQSICDELG
                     VAYERHDTTLKSLVDALNRHLLQSHAAGRRTVLIIDEAQNLAPEVLEQVRLLTNLETH
                     KDKLLRIMLVGQPELAALLARPDLRQLAQRITARYHLTPLDSRETAEYVLHRLRHAGA
                     QNELFDAQSLMEVYRRTRGIPRLINILCDRALLGAYAAGAMRVNARLVRAAASEAAGN
                     LKPQRPLARFLVRTEKIWAALALLLAVALVWKSAHEADWVPAPPFVQPTVGQSLTLAS
                     SLSRPPVSAPAADAAGNPQLRQLLAAAQPLPVAMGRLIGLWDRRVRLSGDDNVCRELS
                     SHKLECYRGHGSWNQLAQLNRPALITLRTPFGDERHVLLRGLSATHAVLETAGGTQKL
                     PLALLDGLWTGDYLLLWRNDSGQTRVGPETRGEPVFWLARRLVQLGLLPASSSVNDRY
                     DANLRNAVSKFQSAHGLPADGVADIATLIALSDGPRLEGSA"
     gene            complement(9455..10927)
                     /locus_tag="VHE37_02860"
     CDS             complement(9455..10927)
                     /locus_tag="VHE37_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34497.1"
                     /translation="MRMRRLLCAAGALAIPVSAYAHGFGRLYNLPVPFWLYAWAAGAA
                     LVLSFLLIAWFATAPPAPASSAARDISNTWCVRALRWVRPLLRTLAVSALLLCMATGW
                     WGSVDPYRNFSMTSFWIVFVLGCTYLTALSGNFYAALNPWKTLADGIARRWARYAQGR
                     VRYAESLGDWPALALYLGFVWFELFNHGYPSTLAWMLFAYSLLNLAGVWWVGAPAWFA
                     HCEFFSVFLRLVGVMAPLDYQPGYQPGAPHGTLRWRLPLSGVMHERPAHLSTVIFALA
                     MLSTTAFDGLRATQWWVGLVWRDPTGWLTALLGTKPIMVYPMLRPWYIAWESFWLLAS
                     PFIYAAAYCTALLAGKLLTRSARPLRELLLDFGYTLLPIALVYNLAHYSTLVLGQGMK
                     IISLLSDPFGRGWNLFGTAHLLRAPILPGMGIVWHSQVLLILLGHVLGVYAAHRVALR
                     VWPSRGAALASQLPMLLLMLALTVSGLWILAQPLTDLLMR"
     gene            complement(10948..13158)
                     /locus_tag="VHE37_02865"
     CDS             complement(10948..13158)
                     /locus_tag="VHE37_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34498.1"
                     /translation="MGLNVYRCGLASVLIAAAVSGCSSSDSPAPAAAGGGGSVSAAPV
                     SRYDMANRCFVLRANGGYAGRNGGSFVASATSASGAEHFFMKPAGLGRYVFYTSDQLF
                     MTASGTAVSAADAPGDGSDWTVDGDNGVFTASTLGQKLALDSSGNVVLGDTAALLTFE
                     PASGCTDYPEMPVDVDSPTFKSNPVGRPVIGFAEVHSHMGMGSEMSDGANPVGPSAGG
                     VMYGQAINRFGVIEALKDCSAMHGPDGVLSPENIILDQQPLEHHQTKGWPTFVDWPKN
                     NSELHQQMYYRWVERAYRAGLRTMVIHGTNIEALCDIAKATVGDKNADCADMSVGEKQ
                     VKYLTGIQDYVDAQEGGPGKGWFRIIKDPAEARGVISEGKLAVIPGLEFSNLFHCNVT
                     FNPLGGETSACTKDDIDAGVEEAWNLGVREVFPFHDVDSALGGTGIFSSILDYVGFVG
                     THGFWKMYPCPDVDYFTGEGVYVAGAEMQTASLTQFNDPITQAIIQYGAGILPVYGPG
                     RQCNARNVTDLGKYAIDKIMKKGFVLDIDHAELASKQYMLDQGARTTPYYPMVSGHGG
                     HGGITTAQATQIIKQGGIIYPALPNGKDFARFIQQIKPVWAASGTTRPLAVGYGADSN
                     GLRDLPGPRGAGSKPIPYPFTLFQGDGWGPEYAAAGIAPIKIEQLSIPGGRSWNMDEE
                     GMSHYGLVPDIVEEISIEGGAEATSALYRSSEAYLELWEQTLQAAASARQLPSP"
     gene            complement(13258..>13751)
                     /locus_tag="VHE37_02870"
     CDS             complement(13258..>13751)
                     /locus_tag="VHE37_02870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT34499.1"
                     /translation="HHFAGCGGVFSDMNAHWTWYRWNELTPDTLHALLKLRSDVFVVE
                     QDCVYADIDGRDPQCEHLCASDGQGVLLACLRLVPPGVKYELPAIGRLVVAPPARKLG
                     LARAGMRLAIERCAQRWPRSPVFLQAQQYLQPFYASLGFAASGEPYVEDGIPHIDMVM
                     HTH"
BASE COUNT         2245 a         4685 c         4572 g         2249 t
ORIGIN      
        1 accatttttg gccttaccgc actgacattt cggatgttat taaatattta ccatttttac
       61 ttttgatata tattttataa catcaaaaat ggccgtgcgg ttgacgcatg cacgcgcctt
      121 ggtacgaagc tgcggggtcg aatcaggtga cgaaaccgcg gatcaacgcg ttcacgcgct
      181 cgggaaactc gtgcgccacc cagtgcgtgg cctgctcgat gcgctcgatg cgcaggtcgg
      241 gcacccattg ctccaggcca tcgagcagcg ccggcgtcag cgcgctgtcc tgcatgcccc
      301 agatgaccag cgtgggcacg cgcacggtga actgcgcgcc gtcaagcttg aggccggcag
      361 cgttggcgga tgtttccgac ggcgggcgca gcggcgaggc gcggtagtag ttgagcccgg
      421 cggtgagcgc gccgggctgc gtccatgcct gcatgtagag atcgagatct tcctgcgtcg
      481 gcggccgcgc gcttttcttc cagccgtcga gctgcgcgcg caggcggcgg taatcgtcct
      541 gcatcagcac gcgctcggcc ttggcatcag ccagcagcag catgtaggcg ctcgccgcct
      601 gctgcgcggg attgcgcgcc agctcgcgca tgtacggcat cgggtgcggc gaattgagga
      661 tgaccagctt ctccaccatc tccgggtgct gcgccgccag gttccaggcg atggcgccgc
      721 cccagtcgtg cgccaccacg atcgccttgc cgtggcccag ctgttcgatc agctggcgca
      781 tgtcctcgac cagttcgcgc gcgtggtacg cggaaacgtc ctgcggcttg ctggagagat
      841 tcaccccgcg cgtatcgggc gccaccacga aatggtcgcg cgaaaattcc gccagctgtt
      901 ccttccacgc gtaccagaac tccggccagc cgtggatgaa cagcatcagc ttgccgtgac
      961 ccgccgcggc gtagtgcagc cgcacgccgt tgacctcggc ataacgatgg gcgatgtgat
     1021 tactgctcat gcggccagca tcaggtcgct gtagcgcgcc aggtgatggg cagcgtcgcc
     1081 gaaggtgaga tcgatcatgg tcagccgctt gaagtaatgg ctgacgttga gctcgtcggt
     1141 cacgccgatg ccgccgtgca gctgcaccgc ctgctggccg acgtggcgcg cggcctggcc
     1201 gaccagcgcc ttggcggcgg cgacggcgcg gcggcgctcc tgcaggtccg ctgagccgat
     1261 gcgcgcggca atgagatatg ccatggaacg cgccgactcc agctgcatca gcatgtcggc
     1321 catgcggtgc tgcagcgcct ggaaactgcc gatgggcacg ccgaactgct tgcgcgtctt
     1381 caggtaggcg ccggtgatct ccagcagcgc gcccatggcg ccgaccgctt ccgcgcacag
     1441 cgcggcaatg gcgtgctgca gctggtgctc gatggccggc agcgccgcgc ccgcctggcc
     1501 gagcagcgcg tcggcgctca cctgcacgcc cttgagcgtg atgtcggcgg cgcgctggcc
     1561 atcctgcgtc gggtagccgt ggatcgacac gccggaggat ttcggatcga ccaggaacag
     1621 cgagatgcca gcttcgtcgc gctcgccacc ggcggttctc gcggacacga tcagccggtc
     1681 ggccgctgcc ccgttgagta ccacggtctt ggcgccgtca agcacgtagc cgttgccgct
     1741 tgctacggat ttcgtagcaa catgcgccag ctcatagcgc gcaccgggtt cctggtgcgc
     1801 cagcgccagc tgcagttcgc cggacgccac cttgggcagc agcgcggact tctgcgcgcc
     1861 actgccgaca tgcgcgatca ggtcggcggc cagcaccacg ctggccaggt agggctccac
     1921 caccagcgcg cggccgaagg cctccatcac cacgtaggtg tcgaacggac cacccaggcc
     1981 gtatccgccg taggcctcgg gcaggccgat ggccgtaagt cccatctccg cgaactgctt
     2041 ccagtgcgcg cggctccagc cgtcggtcga gcggatgatc tcgcgccgct gctcgaagcc
     2101 atagttcttc tgcaggtagc gcagcagcgt gtcgcgcagc gcggtttgtt cttcggagaa
     2161 ttcgaagttc atgtttcaga gtcccagcat catgcgcgca atgatgttgc gctggatttc
     2221 gtccgagccc gcgtagatcg aggccttgcg gaaattgaag tagtgcccgg gcaatggcgg
     2281 gccgtactcg ggcccggcca gcgggccgcg gtaatcggcg tcgagcgcgg cgcgctggta
     2341 agccagcgcc tgcgggccca gcgccagcat catcagctcg gtcaacgcct gctggatctc
     2401 cacgcccttg accttgagca tcgacgcctc cggccctggc gccttgcgct gctgctccat
     2461 ctcgatcacg cgcaggttgg tgatctccag cgccatcagg ttgatctcca cctgcgccac
     2521 gcggtcacgg aagcgcggat cctgcatcag gctgcggccg ccgtcggccg gctgccggcg
     2581 cacgatctcc ttcaggtgat tgagccagcg cttgcacatg ccgatgctgg cgttgccggt
     2641 gcgctcatgc ccgagcagat acttggcgta ggtccagccc ttgttttcct cgccgaccag
     2701 gttggccgcg ggcacttcca cgttttcgaa ccacacctcg ttaatctcgt gctcgccgtc
     2761 gagcatgatg atcggccgca ccgtgatccc cctgctcttc atgtcgatca gcaggaagga
     2821 aatcccctcc tgatggcgaa cctccgggtt ggtgcgcacc aggcagaaga tccagtcggc
     2881 gaactgcgcc agcgtggtcc aggtcttctg gccgttgacg atgtacttgt cgccctggcg
     2941 caccgcgcgc gtcttcagcg aagcgagatc ggaaccggag cccggctcgg aataaccctg
     3001 gcaccaccag tcctcgcccg agcggatgcg cggcaggaaa tgctcctgct gcgcctggct
     3061 gccgaaggcc atgatcaccg gcgccaccat gcgcaggccg aacggcatta gcgccggcgc
     3121 gccgcccagg gcgcattcct cctcgaagat gtgctgctgc gtggcgttcc agccggtgcc
     3181 gccgaaccgc ttcggccagg tcgacgccac ccaacccttg cggtgcaggg tcttgtgcca
     3241 gtcgagaaaa tcttcgcgcg tgatgcgctt gccgtgcagc accttgtcgc ggatcgccgg
     3301 cttgaggttg tgcgcaacga actcgcgcac ctcggcgcgg aaacgctcgt cctcggcggt
     3361 gaaattcaga tccatgctcg cgccccgcgc ctgaatgaag agcggtgagc ttaggagcgc
     3421 attgaccatc gggcaaatga atgttccgca tcgcgcggca tcaaatgggc taatgcagta
     3481 gattcctgcc atgaaactga ccaaggccta tcgcaacgcc gagttcctca gcagcgagga
     3541 cgcgcgctcc atccgcatcc tcgccgaata cctggaacca gccagccgct tccgccgcaa
     3601 gaacatccgc aaggcgatca tctttttcgg ctccgcgcgc atcaacaagc tcacgcgccg
     3661 cacctcgcgc tactaccgtg aagccgtcgc gctggcggag aagctggcac gctggaccac
     3721 ccaggtgcac gcaaagcccg agcgctacca catctgcacc ggcggcggcc ccggcatcat
     3781 ggaagcggtg cacgaaggcg cggccaaggt ggaccgcaag ctcaacatcg gcctgaacat
     3841 ctcgctgccg ttcgagcagc acgtgaatcc gcacgtgatc aagggccacg cgctggagtt
     3901 ccactacttc ttcatgcgca agttctggtt cctcaacgtg gcgcacgcgg cggtgatctt
     3961 ccccggcggc ttcggcacgc tggacgaact gttcgagctg ctcacgctga cccagaccgg
     4021 caagtcgtcg ccgatgccga tcgagctcta gggaaaaaaa ttctgggaag agaccatcaa
     4081 cttccgcgaa ctgctgcggc tgggcctgat cagcgcaacc gacctcaagc tgttcaaggt
     4141 gtgcgacagc gtggaccaag ccttcgacgc gctggccacg ccgctgcagg cgcagtcgcg
     4201 gcgcaaggcg ccgggcggcg cggtcgacct ttcaaaacca tcgcactgat gaaattttcc
     4261 cgcgcgccgg cacccaggcc ggtgctaagg tgctgcgcac aagcgccgac gtagagcgct
     4321 gctcaacgca agggggaaag atgggattcg atccgctgtt gctgctcgcg ctgctgggat
     4381 tccggcgcaa gcgccgccgc aagccggcta ccggctgacg ccgggccaaa taaaaaaccg
     4441 ccggtccggc ggttttttat ttctggcgcg ggtggcgcga ggtgggcagg tcgtcgatgt
     4501 cgaggtcgtc gtcgatcagc ctgcgcagcg acaattcctc cttgtatttc tccacatcgc
     4561 gccagtcgcg cattttttcg gcggcatgtt cctcaacctc ggccacttcc tcttcggcaa
     4621 cgtcggactc gtcgccgaac tcgtccaggt tcttggcggc gtcgggctgc ggctgcggtt
     4681 tcttgggttt gcgcggcatg gtcggaagca ggtgcggaac gggttcgtaa tacaggcggg
     4741 cgttcaaagt ttgcggccgc tatttagcag ggtttccggc gctttggaag tcccgcccag
     4801 cttgcgccgg caggggcgca atccgcaccg gcactcaggc gaaaaacctc gccgttccgt
     4861 tctggtgttt tgcaggggtc atcactagaa tgtgcgcccc tgatggtccg gtgtggggcg
     4921 gtagctcagc tgggagagcg tcgcgttcgc aatgcgaagg tcgggagttc gatcctcctc
     4981 cgctccacca atcatcagaa tgtttagaat cgacaaggcc cggcgacggg cctttttgca
     5041 ttgcgtgtcg aggtgaccca tggcgagcgt gacgcgacag accttcaacg aggtgatggt
     5101 tcccaattac cagccggccg acatcatccc ggtgcgcggc gccggctcgc gcgtgtggga
     5161 ccagcagggc cgcgaataca tcgacttcgc ctgcggcatc gccgtcaccg gcctgggcca
     5221 ctgtcatcca agaattgttg ctgcgttgac cgagcaggcc aacaagctgt ggcacctgtc
     5281 gaatgtgatg accaacgagc cggcgctgcg gctggcgaaa aagctgaccg agctgacctt
     5341 cgcgcagaag gtgttcttct gcaattccgg cggcgaagcc aacgaggccg cgttcaagct
     5401 ggcgcgccgc tacggcaaca cgcagttcgg tccgcacaag caccagatca tttccttctt
     5461 caacgccttc cacgggcgca cgctgttcac cgtgagcgtg ggcgggcagg ccaagtacac
     5521 cgcgggcttc gagccgattc ccggcggcat catccacctg ccgtacaacg acatcgccgc
     5581 gctggaagcg gcggtgggcg agcagacctg cgcggtggtg gtggaactgg tgcagggcga
     5641 aggcggcatc acgccggccg atcccgcttt catcaagcgc gcgcgcgaac tgtgcaacga
     5701 gcacaaggcg ctgctgatct tcgacgaggt gcagaccggc aacggccgct gcggcccgct
     5761 gtattgctac cagcattacg gcgtgacgcc cgacgtgctg accaccgcca agggcctggg
     5821 cgcaggcttc cccatcggcg ccatgctcac caccgatgat tgcgccaagg tgctggcctt
     5881 cggcacgcac ggctccacct acggcggcaa cgcgctgggt tgcgcggtgg ccgaagcggt
     5941 gctggatgaa ctgagcaagc ccgagctcgt cgccaatgcc agcgcgcgcg caacgcagct
     6001 gcgcgatggc atcgccgcca tcggcaagcg ctacggcatc ttcgacggct cgcgcggcct
     6061 gggcctgctg atcggcgcgc cgatgagcgc cgcgtggaaa ggcaaggcca aggaagtggt
     6121 caacgccggc ctgcgccacg gcctgtggtg cctgatcgcc gggccggacg tgctgcgcct
     6181 ggcgccggcc ctcaacatcg gcgaggcgga agtggccgaa ggcctcaagc gcctggacgc
     6241 cgcctgcgct gagctggccg cgctgcccaa agtctccaac ggctgacaag cgcttgcggc
     6301 gattgctgcg gggtgcggcc gctatcatgc ggccgtcatt ccatggaggc cgcgcatgtc
     6361 cgatcgccga ttgttgctgc tggccgcagc gctgctgacc ggctgcgccg ccggctatgt
     6421 cgaacccgac gtcagcccca ccgtcgccta cctcacgctc gagccgggcg acatgaacgg
     6481 cgccacccgc atcgtctact cggcgttcca cgatgccgcc tgcaccccgc gcgagggcga
     6541 tggcgcggtc gccaccgtgg gcctgggcgc cggcccggcg aagaccatcc gcgtgttctc
     6601 cggcgggccg atctacatcc gcgcgctgcg ccagatcacc gtggccaacg gcacgctgcg
     6661 cagctgcgtc aacctgatca gcttcgtgcc gcagcccgac cgccagtacc gcctgatgcc
     6721 ggagaccgac gccgaccggc cgtgcgaact cacgctgatc gacgcgcaca ccgagaagaa
     6781 cgaagccagc ctgaagcgcc tgcagccggg cctggcctgc agcgcgccct agcgcgcggc
     6841 gctgctacga aatccgaagc acgcggcgcc gccgcgcgcg aaagcgcact ggcagcggaa
     6901 aatgggctcc accgcacggg ctttttgcct gttataaaat atttatcgtt taaataaaca
     6961 gataatgttt tataacacca ccaaggccgg tgcggtggcg gccttccgcg cggttttccg
     7021 ccactgcgaa cctgcagtca atgcggaatc ggcaacagca cctgccggcc gctccagcgc
     7081 agcaccacgc cgccctcggc gatctgctct acctgcggcc cttcgcgcag cgcgtcgccc
     7141 tcgcggtagc ggtgcccgct gatcatgacg aagcgcttgg ccggatcgtc gtcgtacacg
     7201 tgcacgtcga gcgtgagcct ggggaagttg gcgcggaaat ccggcggcat gtccttcagc
     7261 tcgggaatct gcttcggcgg cgccggcgcc agcttcaggt gttcgacttc gagcttgggc
     7321 ggcggcgctt cggcctgcgc gtcgtcggcc gcaacctcgt cgccggccgg tgccgatgcc
     7381 ggttgcgccg gcgcggtgac ttccgccggc agcggcgccg aaggttcggc gggcgcattg
     7441 ctcgcgctat tgctgtcagc caggtcgtcc aggctgttga tggtctgcgc cgcctgcggc
     7501 gggatcacct cactctcttc cgccggcgcc ggcggcgctg cagcatccac cggggcgggc
     7561 gccggaaccg ctgcgaccac cgcaggcgca gcggctgcgc gcggcttgta cagcagcgcg
     7621 atcagcagcg cgccgcaggc gaacgcgagc agcacgcaca cggcgatcag cggccacggc
     7681 agcacgcgcg cttccggcgc cgacaccgcc ggcggcggct gcaggccgtt ctgcacggcg
     7741 ttgcgttcct gctccgcgcg gcgcagcgca tcgagcagca ggctcatgcg ctgccctcca
     7801 ggcgcggacc gtcggacagc gcgatcagcg tggcgatatc ggccacgcca tcggccggca
     7861 ggccatgtgc gctctggaac ttcgacaccg cgttgcgcag gttggcgtcg tagcggtcgt
     7921 tgacgctgct gctggccggc agcaggccca gctgcaccag ccgccgcgcc agccagaaca
     7981 ccggctcgcc gcgcgtctcc ggccccacgc gggtctgccc actgtcgttg cgccacagca
     8041 gcaggtaatc gccggtccac aaaccatcga gcagcgccag cggcagcttc tgcgtgccgc
     8101 ccgccgtctc cagcacggca tgcgtggccg acagtcccct cagcagcacg tggcgctcgt
     8161 cgccgaacgg cgtgcgcagc gtgatcagcg ccgggcggtt gagctgcgcc agctggttcc
     8221 agctgccgtg gccgcggtag cactccagct tgtgcgatga cagttcgcgg cacacgttgt
     8281 cgtcgccgga cagccgcacg cgccggtccc acaggccgat cagccggccc atcgccaccg
     8341 gcagcggctg cgccgcggcc agcagctggc gcagctgcgg attgcccgcc gcgtcggccg
     8401 ccggcgcaga aaccggtggc ctggacagcg acgacgccag cgtcagcgac tgcccgaccg
     8461 tgggctgcac gaacggcggc gccggcaccc agtcggcctc gtgcgcgctc ttccatacca
     8521 gcgccacggc gagcagcagc gccagcgccg cccagatttt ttccgtgcgc accaggaagc
     8581 gcgccagcgg acgctgcggc ttgaggttgc ccgccgcctc gctcgccgct gcgcgcacca
     8641 gccgcgcgtt gacgcgcatg gcgccggccg cgtatgcgcc cagcagcgcg cggtcgcaca
     8701 ggatgttgat caggcgcggg atgccgcgcg tgcgccggta cacctccatc agcgattgcg
     8761 cgtcgaacag ctcgttctgc gcgccggcgt ggcgcaggcg gtgcagcacg tactcggcgg
     8821 tctcgcgcga gtccagcggc gtcaggtggt agcgcgcggt gatgcgctgc gccagctggc
     8881 gcaggtccgg ccgcgccagc agcgcggcca gctccggctg gccgaccagc atgatgcgca
     8941 gcagcttgtc cttgtgcgtc tccaggttgg tcagcaggcg cacctgttcc agcacttccg
     9001 gcgccaggtt ctgcgcctcg tcgatgatca gcaccgtgcg ccgcccggcc gcgtggcttt
     9061 gcagcaggtg gcggttgagt gcatcgacca gcgacttcag cgtggtgtca tgccgctcgt
     9121 aggcgacgcc gagttcgtcg cagatcgact gcacgaattc ctgctcgctc tgccgcgggt
     9181 tgtggatcat ggccacgtcg accgcggcca gcttctgcgt gagcagcgcg cgcaccacgg
     9241 tggtcttgcc ggtgccgacc tcgccggtca gctgcacgaa gccgccgtac tgcccggtgc
     9301 cgtacagcag gtggccgagc gcctcctggt ggcgcggcga caggtacagg taggcggggt
     9361 ccggggtaat ggagaacggc atctcccgga ggtggaaatg ttcgaggtac acggcggcgg
     9421 ctagtgtagt cacctgcgcg ccgcggcgca ggattcagcg catcagcaga tcggtcagcg
     9481 gctgcgccag gatccacagg ccgctgacgg tcagcgccag catcagcagc agcatcggca
     9541 gctggctggc cagcgccgcg ccgcgcgacg gccacacgcg cagcgccacg cggtgcgcgg
     9601 cgtacacgcc cagcacgtgg ccgagcagga tcagcagcac ctgcgagtgc cagacgatgc
     9661 ccatgcccgg caggatcggc gcacgcagca ggtgcgcggt gccgaacagg ttccagccgc
     9721 ggccgaacgg atcggacagc aggctgatga tcttcatgcc ctggccgagc accagcgtgg
     9781 agtagtgcgc gaggttgtag accagcgcga tcggcagcag cgtgtagccg aaatcaagca
     9841 gcagttcgcg cagcggccgc gcgctgcgcg tgagcagctt gccggccagc aatgcagtgc
     9901 aataggcggc ggcgtaaatg aacggcgagg ccagcagcca gaaactttcc caggcgatgt
     9961 accacggccg cagcatcggg tagaccatga tcggcttggt gccgagcagc gcggtgagcc
    10021 agccggtggg atcgcgccag accagcccaa cccaccactg cgtggcgcgc aggccgtcga
    10081 acgcggtggt cgacagcatg gccagcgcga agatcaccgt gctgaggtgc gccggccgct
    10141 cgtgcatcac acccgacagc ggcaggcgcc agcgcaaggt gccgtgcgga gcgccgggct
    10201 ggtaaccggg ctgatagtcg agaggcgcca tcacgccgac caggcgcagg aacaccgaga
    10261 agaattcgca gtgcgcgaac cacgccggcg cacccaccca ccacacgccg gcgagattga
    10321 gcaggctgta ggcgaacagc atccatgcca gcgtcgatgg atagccatgg ttgaacagct
    10381 cgaaccagac gaagcccagg tacaacgcca gcgcgggcca gtcgcccagc gattccgcgt
    10441 agcgcacacg cccttgcgcg tagcgcgccc agcggcgcgc gatgccatcg gcaagcgtct
    10501 tccagggatt gagcgcggcg tagaagttgc cggacagcgc ggtcaggtaa gtgcagccca
    10561 gcacgaacac gatccagaag gaggtcatgc tgaagttgcg atacggatcg acgctgcccc
    10621 accagccggt ggccatgcac agcagcagcg ccgagacggc gagcgtgcgc agcagcggcc
    10681 gcacccagcg cagcgcgcgc acgcaccatg tgttgctgat gtcgcgcgcg gcactcgatg
    10741 ccggcgccgg cggcgcggtg gcgaaccagg caatgagcag gaacgacagc accagcgccg
    10801 cgcccgccgc ccaggcgtaa agccagaacg gcaccggcag gttgtacagg cggccgaagc
    10861 cgtgtgcgta cgcgctgacc ggtatggcca gcgcgcccgc cgcgcacagc aggcggcgca
    10921 tccgcatgcg cgccgctcgc ggaccggtca gggtgagggc agctgcctgg ccgaggccgc
    10981 ggcctgcagc gtctgctccc acagttcgag gtaggcctcg gacgagcgat acagcgcgct
    11041 ggtggcttcg gcgccgccct cgatgctgat ttcctcgacg atgtccggca ccaggccgta
    11101 gtgcgacatg ccttcctcgt ccatgttcca gctgcgcccg cccgggatgg acagctgctc
    11161 gatcttgatc ggcgcgatgc cggcagcggc gtattcaggc ccccagccat cgccctggaa
    11221 cagcgtgaac ggatacggga tcggcttgct gccggcgccg cgcgggccgg gcaggtcgcg
    11281 caggccgttg gagtcggcgc cgtagccgac cgccagcggg cgcgtggtgc cggacgcggc
    11341 ccataccggc ttgatctgct ggatgaagcg cgcgaaatcc ttgccgttgg gcagcgccgg
    11401 ataaatgatg ccgccctgct tgatgatctg cgtggcctgc gcggtggtga tgccgccgtg
    11461 gccgccatgg cccgacacca tcgggtaata cggcgtggtc ctggcgccct ggtcgagcat
    11521 gtactgcttg ctggccagct cggcgtgatc gatgtcgagg acgaagccct tcttcatgat
    11581 cttgtcgatg gcgtacttgc ccaggtcggt cacgttgcgc gcgttgcact ggcgccccgg
    11641 accgtacacc ggcaggatgc cggcgccgta ctggatgatg gcctgcgtga tcggatcgtt
    11701 gaactgggtc agcgacgcag tctgcatctc cgcgccggcc acgtacacgc cctcgccggt
    11761 gaagtagtcg acgtccgggc acgggtacat cttccagaag ccgtgcgtgc cgacgaagcc
    11821 gacgtagtcg aggatcgagc tgaagatgcc ggtgccgccc agtgcgctgt cgacgtcgtg
    11881 gaacgggaac acctcgcgca cgcccaggtt ccacgcctcc tcgacgccgg cgtcgatgtc
    11941 gtccttggtg caggccgagg tctcgccgcc cagcggattg aacgtgacgt tgcagtggaa
    12001 caggttggag aattccaggc ccgggatcac cgccagcttg ccttcgctga tcacgccgcg
    12061 cgcctcggcc gggtccttga tgatgcggaa ccagcccttg cccgggccac cttcctgcgc
    12121 atcgacgtag tcctggatgc cggtgaggta cttcacctgc ttctcgccca cgctcatgtc
    12181 ggcgcagtcg gcgttcttgt cgcccaccgt ggccttggcg atgtcgcaca gcgcctcgat
    12241 gttggtgccg tggatcacca tggtgcgcag cccggcgcgg taggcgcgct ccacccagcg
    12301 gtaatacatc tgctggtgca gctcggagtt gttcttcggc cagtcgacga aggtcggcca
    12361 gcccttggtc tggtggtgct cgagcggctg ctggtcaagg atgatgtttt ccggcgacag
    12421 cacgccgtcg gggccgtgca tggccgagca gtccttcagc gcctcgatca cgccgaagcg
    12481 gttgatcgcc tggccgtaca tcacgccgcc tgccgacggc ccgaccgggt tggcgccgtc
    12541 ggacatctcg ctgcccatgc ccatgtgcga gtgcacctcg gcgaaaccga tcaccggccg
    12601 gcccaccggg ttgctcttga aggtgggcga atcgacgtcc accggcatct ccgggtaatc
    12661 ggtgcagccg ctggccggct cgaaggtgag cagcgccgcg gtatcgccca gcaccacgtt
    12721 gccgctgctg tcgagcgcca gcttctggcc cagcgtgctg gcggtgaaca cgccgttgtc
    12781 gccatcgacg gtccagtcgg aaccgtcgcc cggcgcatcg gccgccgaca ccgcggtgcc
    12841 gctggcggtc atgaacagct ggtcgctggt gtagaacacg tagcggccca ggccggccgg
    12901 cttcatgaag aaatgctcgg cgccgctggc gctggtggcg ctggccacga aggatccgcc
    12961 gttgcgcccg gcatagccgc cattggcgcg cagcacgaag cagcggttgg ccatgtcgta
    13021 gcgcgacacc ggcgcggcgc tgacgctgcc gccgccgcca gccgcagcag gtgccggcga
    13081 atcggaagaa ctgcagccgg acacggccgc ggcgatgagc acggacgcca gtccgcagcg
    13141 gtagacgttc aaccccatga ctctctcccc cggcgcaaaa gccgatgatc ggaatttcaa
    13201 gcgggcggat tatgacggca atgtgtctgc cacggacggg cgttgatcgt tttttgccta
    13261 gtgcgtatgc atcaccatgt cgatgtgcgg gatgccgtct tcgacgtacg gctcgccgct
    13321 ggcggcaaat cccagcgagg catagaacgg ctgcaggtat tgctgcgcct gcaggaacac
    13381 cggcgaacgc ggccagcgct gcgcacagcg ctcgatcgcc agccgcatgc ccgcgcgcgc
    13441 cagcccgagc ttgcgcgccg gcggcgccac caccagccgg ccgattgccg gcagctcgta
    13501 cttcacgccg ggcggaacca ggcgcaggca tgccagcagt acgccctgcc cgtcgctggc
    13561 gcacaggtgc tcgcactgcg gatcgcggcc gtcgatgtcg gcgtagacgc agtcctgctc
    13621 caccacgaac acatcgctgc gcagcttgag cagcgcgtgc agcgtgtcgg gagtgagctc
    13681 gttccagcga taccaggtcc agtgcgcgtt catgtcggaa aacactccac cgcaccccgc
    13741 aaagtgatgt t
//
LOCUS       DASZZC010000122         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_329440,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000122 DASZZC010000000
VERSION     DASZZC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329440"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VHE37_02875"
     CDS             complement(<1..1222)
                     /locus_tag="VHE37_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT34500.1"
                     /translation="MRHAGWLLLALPLAAHAVDAVTPAPTPAQELLLSARLWESQSRG
                     DLAQVALEKYLRAVPDDPRAMLMLGEIEIRSARPQDAAKVLERLRAAHPQDPAVRSLE
                     DAYRVGTRDRLKMATVVRLTQIGQDDEAVAQLRALFPSGAPGGELGVDYFRILASSDK
                     TRLEAESGLRGLVRDNPGDPLFQLALANFLVAREATRPDGLRMLAALGARHDVDHTRV
                     MDAWRDGAQRAVGEVRSARLVQQYLAAAPDDAETRKQVALAERAQAQARAQAADPIVQ
                     LTARGVDDLDHGRLDLAETELSQARARRPASGEIAGALGRLRLRQDRFEEARPLFEEA
                     ARNDKAHATRWRSLVAETDFQRTLRDADTALAAGQPDRAGGLAQAALKQRPHDADALA
                     LSADVARAQHDAGAE"
     gene            complement(1219..2298)
                     /gene="bcsZ"
                     /locus_tag="VHE37_02880"
     CDS             complement(1219..2298)
                     /gene="bcsZ"
                     /locus_tag="VHE37_02880"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HVT34501.1"
                     /translation="MWLAAAGAHADCGSWPQWEDFRHDLLSADGRVIDASTARQHSTS
                     EGQSYALFFALVANDRDTFERVLQWTGNNLAAGDLSSRLPAWQWGRRDDGSWGVIDAN
                     AASDADVWIAYSLAEAARLWNVRRYRILSKAIAQRILAEETATLPGLGLVLLPAPQGF
                     TPAPGKWRLNPSYLPLQALRGLARYDGRWNALAASSAHVIVDSAPRGYAPDWTLYDGS
                     HFLPDADTRGIGSYNAIRVYLWAGMLDAADPARAVLLRALNPMAQYVAGHRAPPESVE
                     VSTGETHNDGPAGFSGALVPFLSALADSSGAATQATRARQSLGGLPKPYYGSALNLFG
                     LGYAEGRYRFDAGGQLHPRWEGVCK"
     gene            complement(2345..>3175)
                     /locus_tag="VHE37_02885"
     CDS             complement(2345..>3175)
                     /locus_tag="VHE37_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HVT34502.1"
                     /translation="DSEADAQRSAIDPDSTIDLRRFPHYTAMPNLALFANAGFPFSKY
                     ADLAETALVLPDQPSATDLEAALFLLGRMGRVTGVPAQRFTLAHAAEAGRLADFDLLV
                     IDAGSLLEQWKQNLPAVIDKSRRYITPQTPAPSYDREIPDQPRQNPAAWKVDLNSSGP
                     LGALLGFESPIGGKRSVIALTASSADALGSVLDALEDPAGVTQVRGDVAFVRGRDVAS
                     FEIEPKYYVGHLPLWLGLRFMLSDHPLLLALLGILAGLLLALTVHWQLKRIAARRLSP
                     "
BASE COUNT          477 a         1132 c         1105 g          461 t
ORIGIN      
        1 tttccgcgcc ggcgtcgtgc tgcgcgcgcg ccacatccgc gctgagcgcc agcgcgtcgg
       61 catcgtgcgg gcgctgcttc aacgcggctt gcgcgagccc accggcgcgg tccggctggc
      121 ccgccgccag cgccgtgtcg gcatcgcgca gggtgcgctg gaaatcggtt tccgcgacca
      181 ggctgcgcca gcgagtcgca tgcgccttgt cgttgcgcgc cgcttcctcg aacagcgggc
      241 gcgcttcctc gaagcgatcc tggcgcaggc gcagccggcc cagcgcgccg gcgatttcac
      301 cgctggccgg acgacgtgca cgcgcctgcg acagctcggt ctccgccagg tcgaggcggc
      361 catgatccag atcgtccacg cctcgcgcgg tcagctgcac gatggggtcg gccgcctgcg
      421 cgcgcgcctg cgcctgcgcg cgctccgcca gtgcgacctg cttgcgcgtc tccgcgtcat
      481 cgggcgcggc cgcgaggtac tgctgcacca gcctggccga gcgcacctcg ccgaccgcgc
      541 gctgcgcgcc gtcgcgccag gcatccatca cgcgggtgtg atcgacatcg tggcgcgcac
      601 ccagcgccgc gagcatgcgc agaccgtcgg gccgcgtggc ttcacgcgcc accaggaaat
      661 tggccagcgc cagctggaac agcggatcgc cgggattgtc gcgcaccagc ccgcgcagcc
      721 cgctctccgc ttccagccgc gtcttgtccg agctggccag gatgcggaag taatcgacgc
      781 cgagttcgcc gcccggcgcg ccggagggaa acagtgcgcg caactgcgcc accgcctcgt
      841 cgtcctggcc aatctgcgtc aggcgcacca cggtggccat cttcaggcgg tcgcgcgtgc
      901 cgacgcggta ggcgtcctcc agcgagcgca cggccgggtc ctgtggatgc gcggcacgca
      961 ggcgctccag cacttttgcc gcatcctgcg gccgggccga acggatctcg atttcgccca
     1021 gcatgagcat ggcgcgcggg tcgtccggca cggcgcgcag gtatttttcc agcgccacct
     1081 gcgcaaggtc gccgcggctc tggctctccc acaggcgcgc gctgagcagc agttcctgcg
     1141 caggcgtcgg tgcgggcgtc accgcgtcca ccgcgtgcgc ggcgagcggc aatgccagca
     1201 gcagccagcc ggcgtgcctc atttgcacac gccctcccag cgcggatgca gctggccgcc
     1261 ggcgtcgaag cggtagcgtc cctctgcgta gccgagcccg aacaggttca gcgcgctgcc
     1321 gtagtacggc ttgggcaaac cgcccagcga ttgcctggcg cgcgtggcct gcgtcgccgc
     1381 cccggaagaa tctgccaacg ccgacaggaa tggcaccagc gcgccggaaa aaccggcggg
     1441 cccgtcgttg tgcgtctcgc cggtcgacac ctcgaccgac tccggcggcg cacgatgccc
     1501 agccacgtat tgcgccatcg ggttcaaggc gcgcagcagc accgcacgcg cgggatcggc
     1561 ggcatcgagc atgccggccc acaggtacac gcggatggcg ttgtagctgc cgatgccgcg
     1621 cgtatccgca tcgggcagga aatggctacc gtcgtacagc gtccagtccg gtgcgtaacc
     1681 acgcggcgcc gaatcgacga tgacgtgggc cgatgatgca gcgagtgcgt tccagcgccc
     1741 gtcgtatcga gcgagtccgc gcaaggcctg cagcggcagg tagctgggat tgagccgcca
     1801 ctttccaggc gccggcgtga agccctgcgg cgccggcagc agtaccaggc ccagtcccgg
     1861 cagcgtcgcg gtttcctcgg cgaggatgcg ctgggcgatg gccttggaca ggatgcggta
     1921 gcgccgcacg ttccacagac gcgccgcttc agcgagtgaa taagcaatcc acacgtcggc
     1981 gtcggacgca gcgttggcgt cgatcacgcc ccaactgccg tcatcgcgcc ggccccactg
     2041 ccaggccggc aggcgcgatg acaggtcgcc ggccgcgaga ttgttgccgg tccactgcag
     2101 cacgcgctcg aaggtgtcgc ggtcgttggc caccagcgcg aagaacaagg cgtaggactg
     2161 tccttccgag gtgctgtgct ggcgcgcggt gctggcgtcg atcacgcggc cgtcggcgct
     2221 gagcaggtcg tgcctgaagt cttcccactg cggccaggaa ccgcagtccg catgcgcgcc
     2281 ggcagccgca agccacatgg ccgcagccgc tacgcgcgcg tacaagcgca ggcgcacagg
     2341 cggatcaggg cgacagccgg cgcgcggcga tgcgcttgag ctgccagtgc acggtgagcg
     2401 ccagcaagag gccggccagg atgccgagca gcgccagcag cagcgggtga tccgacagca
     2461 tgaagcgcag cccgagccac agcggcaaat gaccgacgta gtacttgggc tcaatctcga
     2521 agctggccac gtcgcgccca cgcacgaagg cgacatcgcc gcgcacctgc gtcacgcccg
     2581 ccggatcctc cagcgcatcg agcacgctgc cgagggcatc ggcggacgat gccgtcagcg
     2641 cgatcacgct gcgcttgcct ccgatgggtg attcgaaacc gagcaatgcg cccaacgggc
     2701 cgctggaatt caggtcgact ttccatgccg ccgggttctg ccgcggctgg tcgggaatct
     2761 cgcggtcgta gctcggcgcc ggcgtctgtg gcgtgatgta acggcggctc ttgtcgatca
     2821 ctgccggcag gttctgtttc cactgttcca gcaggctgcc ggcgtcgatc accagcaggt
     2881 cgaagtccgc cagcctgcca gcctcggcgg cgtgcgccag cgtgaaccgc tgcgccggca
     2941 cgccggtcac gcggcccatg cgccccagca ggaacagcgc tgcttctaaa tccgtagcag
     3001 acggctggtc gggcagcacc agcgcggtct cggccaggtc cgcgtacttg ctgaagggaa
     3061 agcccgcgtt ggcgaacagc gccaggttgg gcatggcggt gtaatgcggg aagcggcgca
     3121 ggtcgatggt cgaatcggga tcgatggccg agcgctgcgc gtcggcctcg ctgtc
//
LOCUS       DASZZC010000123         4685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_138111,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000123 DASZZC010000000
VERSION     DASZZC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138111"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VHE37_02890"
     CDS             complement(<1..1392)
                     /locus_tag="VHE37_02890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HVT34503.1"
                     /translation="MKLFFIEHINNMKIIYKDKTAVRWMGKGLFASLAILLAGTANAA
                     QLSPDLRQLDRVPLAVAAVPIIDKALLEATPRKDRPYTFAVPTSVDLRQGDGVWSDVS
                     PGLVHWRARIAAPGATMLHFRLNHVALPNNAELWVYDSGGKTVQGPYTSADILGGELR
                     TADIVGDVGIVEVRMASNDKPKFTINVADVRYGIRKSLKFGVFPQSGSCEVDVACSQG
                     DQYRSEIRSVAMLTYDTSTTSDECSGALVNDVPQDGQPYIFTARHCGITSSIAPELTV
                     YWLDQKASCGSGSEDLSHPQQHATFVTNDSASDFTLIKMNSPPPSDANVYYSGWDVSD
                     TPPQCGVGIHHPSADAKKISIYKQAATAQQVDTPAIQAWQVFWARGVTEQGSSGSGLW
                     DSNHHIVGVLHGGGSDCTHQSEPDYYGRLGVAWSNNSALRNALDPNDTGAVHLCGRNP
                     GDAVDPNCATTSVV"
     gene            complement(1423..2160)
                     /locus_tag="VHE37_02895"
     CDS             complement(1423..2160)
                     /locus_tag="VHE37_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34504.1"
                     /translation="MATTTPFQAPISDAVPLAAIRVSGADADTFLQGQLTQDLRKLDS
                     SSPLLAGYCSPKGRLLAVMQLARDADGVLLHVHGSVADAVLKRLRMHVLRSKVTLELT
                     PAPALTPEQDQAWRLQQIRAGVPVIYRETQDEFVPQMVNLDHLGAISFDKGCYTGQEI
                     VARLHYLGQLKRRMFRSHAAATSVTPGTPVHVAGEAQAVGMVVDAARDGDGCALSVVL
                     QLAHAASAALHLADTARTPLAAPSAYF"
     gene            2418..2825
                     /gene="sdhC"
                     /locus_tag="VHE37_02900"
     CDS             2418..2825
                     /gene="sdhC"
                     /locus_tag="VHE37_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HVT34505.1"
                     /translation="MNQTSRPAVANRPLSPFMLGPYYKFQLTSVLSFAHRVTGIGLSV
                     GTLLLAGWLVALAAGPDWYRMYSFHMTAWYGKVLLLGWSWSLLYHLCNGIRHLFWDMG
                     KGFDIPVAYRSGYAVVIISALLTAAVWAVALYS"
     gene            2836..3216
                     /gene="sdhD"
                     /locus_tag="VHE37_02905"
     CDS             2836..3216
                     /gene="sdhD"
                     /locus_tag="VHE37_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HVT34506.1"
                     /translation="MNDLRSPLGRARGLGSAKDGVHHFWVQRVSAVALVPLALWFVFS
                     VARLRGADYGMVRHFVHAPSVAVTLILFIAAALYHSALGVQVVIEDYVGGEFKKIVSL
                     LLMKFAHAIVAAAAIFAVLKIAFS"
     gene            3227..>4685
                     /gene="sdhA"
                     /locus_tag="VHE37_02910"
     CDS             3227..>4685
                     /gene="sdhA"
                     /locus_tag="VHE37_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HVT34507.1"
                     /translation="MTYEIVHHEYDALVIGAGGAGLRATLGLAESGLKTANITKVFPT
                     RSHTVAAQGGISAALANMGKDDWRWHFYDTVKGSDWLGDQDAIQYMTREAIPAIIELE
                     HYGLPFSRTPEGKIYQRPFGGMTTEYGKGPPAQRTCAAADRTGHAMLPTLYQQSLKGR
                     APVYVEFFALDLLMDADGACCGVLALDMSTGQLHRFRSHMTILATGGYGRAYFSATSA
                     HTCTGDGNGMVLRAGLPLQDMEFVQFHPTGIYGSGCLITEGARGEGGYLTNQSGERFM
                     ERYAPSVKDLAPRDMVSRAMATEIREGRGVGHEHDHIHLHLEHLGPEVLHKRLPGISE
                     SAKIFAGVDVTKEPIPVVPTVHYNMGGIPTLHTGEVVTLKNGNPDSVVPGLMAVGEAA
                     CVSVHGANRLGSNSLLDLVVFGRSAALRAAETVKPGEKQPELKDGMTEPHLARFDRLR
                     NANGKTSTATLRLEMQRAMQEDAAVFRTGASLKSGV"
BASE COUNT          836 a         1465 c         1476 g          908 t
ORIGIN      
        1 gaccacgctg gtagtggcgc agttgggatc gaccgcgtca ccggggttgc ggccacataa
       61 atgcactgcg ccggtgtcat tagggtccag cgcattgcgc agtgccgagt tattgctcca
      121 ggcaacgccc aggcgtccat agtagtcggg ttcgctttgg tgggtgcagt ccgacccgcc
      181 accgtgaagg acaccgacga tatggtgatt cgaatcccat aggccagagc ccgatgatcc
      241 ttgctcggta actccgcgcg cccagaatac ctgccaagcc tgtattgcag gtgtatcgac
      301 ttgttgtgcc gtggcggcct gcttgtagat gctgattttc tttgcatcgg ccgaggggtg
      361 atgaatgccg acgccgcatt gcggtggtgt gtccgaaaca tcccagcctg aatagtagac
      421 gttggcgtca gatggaggag gtgaattcat ctttatcaat gtgaaatccg aagccgaatc
      481 attagtgacg aacgtcgcgt gctgttgcgg atgactcaaa tcttcgcttc ctgatccgca
      541 ggatgccttt tgatcgagcc agtagacggt caactccggt gcaatcgagc tggttattcc
      601 gcaatggcgc gcagtgaaaa tatacggctg accgtcctga ggaacatcat tgacgagagc
      661 gccgctgcat tcgtcactag tagttgaagt gtcgtaagtc agcatcgcaa ctgagcggat
      721 ttccgatcgg tactgatctc cttgtgaaca ggccacatcc acttcacagc ttcctgattg
      781 gggaaacacg ccgaacttga gcgatttcct gatcccgtac cgaacatcag caacattgat
      841 cgtgaacttg ggcttgtcgt tcgatgccat gcggacttcc acaattccga cgtcaccgac
      901 gatatctgcg gtacgcagtt cgccacccaa aatgtcagcg gaggtataag ggccttggac
      961 cgttttcccg ccgctgtcgt agacccaaag ctcagcattg ttcggtagcg ctacgtggtt
     1021 caatcggaaa tgcagcatgg ttgccccagg ggcggcaatt cgtgcccgcc aatgcacgag
     1081 tcctggcgac acatcgctcc agacgccatc cccctggcga aggtcaacgg atgtaggcac
     1141 tgcaaatgtg tacggtcgat ccttgcgcgg cgtcgcttcg agcagcgcct tgtcgataat
     1201 cggcaccgcg gcaacggcga gcggcacgcg atcgagctgg cgcaggtcgg gcgagagttg
     1261 tgcggcgttc gcggtgcccg caagtagtat tgcgagcgat gcgaacaagc cttttcccat
     1321 ccaccgcacg gctgttttgt ctttataaat tatcttcata ttatttatat gttcgataaa
     1381 aaataacttc ataaaggagg gtgcggtgac tgtgcttgga ctctaaaaat acgcgctcgg
     1441 tgccgccagc ggtgtgcgcg cggtatcagc caggtgtaaa gctgctgacg cggcgtgcgc
     1501 gagctgcagc accacggaga gcgcgcaacc gtcgccgtcg cgcgcggcat cgaccaccat
     1561 gcccactgcc tgcgcttcgc cggccacgtg caccggcgtg cccggggtta cgctcgtcgc
     1621 tgccgcgtgg ctgcggaaca tgcggcgctt gagctggccg aggtagtgca ggcgcgcgac
     1681 gatttcctgc ccggtgtagc agcccttgtc gaaactgatt gcgcccaggt gatcgaggtt
     1741 gaccatctgc ggcacgaatt cgtcctgcgt ctcgcggtag atcaccggca cgccggcgcg
     1801 tatctgctgc aggcgccagg cctggtcctg ctccggcgta agcgcgggtg caggagtgag
     1861 ttcgagcgtg accttcgagc gcagcacgtg catgcgcaaa cgcttgagca cggcatcggc
     1921 cacactgccg tgcacatgga gcaggacgcc atcggcatcg cgtgccagtt gcatcacggc
     1981 gagcaggcgt cccttgggcg agcaataacc cgcgagcaac ggcgatgacg aatccagctt
     2041 gcgcaggtcc tgcgtcagct gtccctgcag gaaagtgtcg gcgtcggcgc cgctgacgcg
     2101 gatggctgcc agcggaacgg catcggaaat tggcgcctgg aaaggtgttg tcgttgccac
     2161 tgcggagtga attttgtcac actagcgccc cttcaaaatc acctcgtccg tgactttacc
     2221 tcccgccgat cccgcaagcg ttgccgacca tccggacgag gtcgtcccgc tggctgatct
     2281 gctcaaatcc accccgccgg cagcggagcc ggtccgggaa attggtggac ggaaggacgg
     2341 ccctgatccc acgcgctacg gcgactggga aaagaacggg cgctgcatcg acttttaaga
     2401 actccaaagg agcggtcatg aaccaaacga gcaggccggc ggtggcgaat cgcccgctgt
     2461 cgcccttcat gctggggccg tattacaagt tccagctcac ctcggtgctg tcgttcgcgc
     2521 atcgcgtcac cggcatcggc ctgtctgtcg gcacgctgct gctggccggc tggctggtgg
     2581 cgctggcggc ggggccggac tggtaccgca tgtattcgtt ccacatgacg gcgtggtacg
     2641 gcaaggtgct gctgctcggc tggagctggt cgcttctgta tcacctgtgc aacggcatca
     2701 ggcatctgtt ctgggacatg ggcaagggct tcgacattcc cgtcgcctat cgttccggtt
     2761 atgccgtggt catcatttcc gccttgctca ccgccgcggt ctgggcggtg gcgctctatt
     2821 cctgaggcca tcgacatgaa cgatctgcgt tctcccctcg gccgcgcgcg cggcctcggc
     2881 tccgccaagg acggcgtgca tcacttctgg gtgcagcgcg tgtcggcggt tgcgctggtc
     2941 ccgctggcgc tgtggttcgt gttctcggtg gcgcgcctgc ggggcgccga ctacggcatg
     3001 gtgcgccact tcgtgcacgc gccgtcggtg gccgtgacct tgatcctgtt tatcgccgcc
     3061 gcgctgtacc actcggcgct gggcgtgcag gtggtgatcg aggattacgt cggcggcgaa
     3121 ttcaagaaaa tcgtctcgct gctgctgatg aagttcgcgc acgcgatcgt tgccgccgct
     3181 gcgatcttcg ccgtactcaa gatcgctttc agctaagaag aacaacatga cttacgaaat
     3241 cgttcatcac gaatacgacg cgctggtcat cggcgcaggc ggcgccggcc tgcgcgccac
     3301 gctgggcctg gctgagtccg gcctcaagac cgccaacatc accaaggtct tcccgacgcg
     3361 ctcgcacacc gtcgcggcgc agggcggcat ctccgccgcg ctggccaaca tgggcaagga
     3421 cgactggcgc tggcatttct acgacacggt gaaaggttcc gactggctgg gcgaccagga
     3481 cgccatccag tacatgacgc gcgaggctat tcccgcgatc atcgagctgg agcactacgg
     3541 cctgccgttc tcgcgcacgc cggaaggaaa aatttatcag cgcccgttcg gcggtatgac
     3601 caccgagtac ggcaagggtc cgccggcgca gcgcacctgc gccgccgccg accgcaccgg
     3661 gcacgcaatg ctgcctacgc tctatcagca gagcctcaag ggccgcgcac cggtctacgt
     3721 ggagttcttt gcgctcgacc tgttgatgga cgccgacggt gcctgctgcg gcgtgctggc
     3781 gctggacatg tcgaccggcc agctgcaccg cttccgctcg cacatgacga ttctggcgac
     3841 cggcggctat ggccgggcgt atttctcggc gacgtccgcg cacacctgca ctggcgacgg
     3901 caacggcatg gtgctgcgcg ccggcctgcc gctgcaggac atggagttcg tgcaattcca
     3961 cccgaccggc atctacggct cgggctgttt gatcaccgag ggcgcgcgcg gcgagggcgg
     4021 ctatctcacc aatcaatccg gcgagcgctt tatggagcgc tatgcgccga gcgtgaagga
     4081 cctggcgccg cgcgacatgg tgtcgcgagc aatggcgacc gagatccgcg agggccgcgg
     4141 cgtcggccac gagcacgatc acatccatct gcatctcgag cacctgggcc ctgaagtgct
     4201 gcacaagcgc ctgcctggca tctccgagag cgcgaagatt tttgccggcg tcgacgtgac
     4261 caaggaaccc attcctgtcg tgcccacggt gcactacaac atgggcggca tcccgacgct
     4321 gcacaccggc gaagtggtca cgctgaagaa cggcaatccc gactccgtgg tgccgggcct
     4381 gatggcggtg ggcgaggcgg cctgcgtctc ggtacacggc gccaatcgcc tgggctccaa
     4441 ctcgctgctc gacctggtgg tgttcggccg ctcggccgcg cttcgggccg ccgagaccgt
     4501 caagcccggc gagaagcagc ccgagctcaa ggatgggatg accgagcccc acctcgcccg
     4561 cttcgaccgg ctgcgcaacg ccaacggcaa gacctccacc gcgaccctgc gcctcgagat
     4621 gcagcgggcg atgcaggagg acgccgcggt gttccgcacc ggcgccagcc tgaagtccgg
     4681 cgtca
//
LOCUS       DASZZC010000124         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_522235,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000124 DASZZC010000000
VERSION     DASZZC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522235"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VHE37_02915"
     CDS             complement(<1..196)
                     /locus_tag="VHE37_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34508.1"
                     /translation="MTAEGKRLPEQGQDRADPAIHATVPELFQQQVRRSGNAAALRCG
                     SRTLTYEAVERQANRIAHTLR"
     gene            387..1349
                     /locus_tag="VHE37_02920"
     CDS             387..1349
                     /locus_tag="VHE37_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HVT34509.1"
                     /translation="MSGIHHAIHASLTRLLAGLGACLLWQACLAADPVPDQSLRMDSA
                     LSAQQAAVVSTAASPSTAATPVAAATEANTSINGDPLLKLGAGDLVSVQVFGRPEMSI
                     SAYVADDGNLTLPLVGAVQVAGLSPSEAAQRVADALKKGEFLVNPQVNITLSQYHSQQ
                     VSVLGEVHTPGRYALESKTNIFDLLAQAGGLSADAADTGYLLRRDRNGDVVRLPIDLK
                     NLTANQQVDGDNRLRGGDSIYVPKASQFYIYGEVHAPNRYRLEPGMTVVEALTVGGGI
                     TERGSERRIEIRRRTNDGKYKTIDAHPTDPVQADDVIRVKERIF"
BASE COUNT          271 a          524 c          490 g          255 t
ORIGIN      
        1 cgcgcagtgt gtgcgcgatg cgattggcct gccgctcgac cgcctcatag gtgagcgtgc
       61 ggctgccgca cctcaacgcc gcagcattgc cgctgcgccg cacctgctgt tgaaacagtt
      121 ccggcacggt ggcatggatc gccggatcgg cgcggtcctg tccctgctcc ggcaagcgct
      181 tcccttctgc cgtcatgcgt ctcctcgcgc atcctgcgct gccggtgtca gttggccggc
      241 cacgcactta gacggcggct cgagcgcgga cagttccatc gttattgcac cgcgctgcaa
      301 atttttcgcg ggtgaactcc gccgaagcag tcagggtcta gtggggtgca cgcagcaggc
      361 acattgctga cttggggaag cgcgacatga gcggcatcca tcacgcaatc cacgcatcac
      421 ttacaagact gctggccgga ctgggcgcct gcctgttgtg gcaggcgtgc ctcgcggccg
      481 atccagtgcc ggatcaatcc ctgcgcatgg attcggccct gtccgcgcaa caggccgcgg
      541 tggtcagcac ggcagcgagc ccaagcacgg ccgcgacgcc ggtggccgcc gccactgaag
      601 ccaacacgtc aatcaacggt gatccgctgc tgaagctggg cgccggcgat ctggtttccg
      661 tgcaggtgtt cggccggccg gaaatgagca tctcggccta cgtggccgat gacggcaatc
      721 tcacgctgcc gctggtgggc gcggtgcagg tggccggcct gtcgccgagc gaagcggcgc
      781 agcgggtcgc cgacgcgctg aagaagggcg agttcctggt caatccgcag gtcaacatca
      841 cgctgagcca gtaccacagc cagcaggtgt cggtcctggg cgaagtgcac acgcccggcc
      901 gttacgcgct ggagtcgaag accaacatct ttgacctgct ggcacaggcc ggcggccttt
      961 ccgcggacgc agcggatacc ggctacctgc tgcggcgcga ccgcaacggc gacgtggtgc
     1021 gcctgccgat cgacctgaag aacctgaccg ccaaccagca ggtcgacggt gacaaccgct
     1081 tgcgcggcgg cgattcgatc tacgtgccga aggcttcgca gttctacatc tacggcgaag
     1141 tgcacgcgcc caaccgctac cgcctcgagc ccggcatgac ggtggtcgaa gccttgaccg
     1201 tcggcggcgg catcaccgag cgcggcagcg aacgccgcat cgaaatccgc cgtcgcacca
     1261 acgacggcaa gtacaagacc atcgacgcgc acccgaccga tccggtgcag gccgatgacg
     1321 tcatccgcgt caaggagcgc attttctaga tggacaggaa tcttcccatg gcggtgcccg
     1381 cgaccacggc gttcattcca cgcgcgtatc tcaacccggg cttcagcttc agccagctcg
     1441 gcgcgatcct gaaggcgtac cgcaggcagt cgtttctgat cctgctgtcg gtgctggtgg
     1501 cggccgccgc caacaccaac ctgatgcccc aaacaaacaa
//
LOCUS       DASZZC010000125        14878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1164574,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000125 DASZZC010000000
VERSION     DASZZC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 14878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14878
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164574"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VHE37_02925"
     CDS             <1..490
                     /locus_tag="VHE37_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34510.1"
                     /translation="PFGYDGHYCAVDRRENPTAAACGYFQSGIRVFDIRDPMHPAEIA
                     YFNPPAQSASKAQLTGSEHASSAVGIDANLSTDWCSSPPRFVAPDQLWVTCQDNGFMA
                     LRFTNGAWPIKDAVTSPAAATAGDTGHYGGAALPGLWLPLLLAALRRRFLASPHAGFR
                     CY"
     gene            complement(601..1713)
                     /locus_tag="VHE37_02930"
     CDS             complement(601..1713)
                     /locus_tag="VHE37_02930"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVT34511.1"
                     /translation="MAVVMRAATSASPIAKIEAKAPPARYARGWHCLGLAAEYKDGKP
                     HGINIFGTRLVIFQGEGGKLNVIDAWCPHMGADLALGKVQGDSVICKFHGWSWGGDGA
                     CNHIPYCERIPPKARVKSWPTCEENKMLFVWNDPEGRAPAPEQAIPRIAPVFSSDWAD
                     WSLVKWTININCRELIDNIADMAHFVSVHGSDNVVYFADLFEGHKATQVMVGSNEKLG
                     GKDEFLTTIASYYGPAYQITHMFGAMNGMPVESILLNSHTPINENSFELRFGVLVKKF
                     PGMTQEQCDAMVKSYVDLTNKAFGEDVEIWHNKVRIDNPILCEGDGPVYQNRQWYEQF
                     YIDADKVPAELKARKFIEIDKGIKQKPEIRHAFDSQ"
     gene            1894..3066
                     /locus_tag="VHE37_02935"
     CDS             1894..3066
                     /locus_tag="VHE37_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HVT34512.1"
                     /translation="MSAVTPQMLVQRAREMVPVLKQRAKQAEEQRKVPDETVAEMQAA
                     GLFRVLQPKKYGGYEMDPKVFYDVCMTLAEGCMSTAWIYGVIGVHNWQIALFDPRAAA
                     DVWSKGTDVRIASTYMPKGQVKPVEGGFRFSGRWGFSSGIAHCDWVFLGGLIFNQGQP
                     PEYRTFLLPKSDYEVVDTWHVMGLKGTGSNDIVVKDVFVPEYRTHKASDGFMGTNPGR
                     DTFTSDLYKLPFGQIFVRAVSSAAIGGLQGALDTFLDFAKVRVGDMGNKTSEQAPAQL
                     AASDAAIAVDEMKLVLNRNFDVLMDKIRRKQPLDDIKQRLHFRTQAAQVVDRCARHAY
                     QLFSACGGRGIFTDFPLYRYMLDIYAARGHYANNPEQFGRNYGGVMLGRDNTDFFI"
     gene            3095..4828
                     /locus_tag="VHE37_02940"
     CDS             3095..4828
                     /locus_tag="VHE37_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT34513.1"
                     /translation="MNEAKGWDQEYDVVVVGSGAGGMTAALCAQAQGLSAVVVEKDKV
                     YGGTTAVSGGGIWIPCNDQMAAAGVPDSYEEALTYMKYLTGGEVPLSRIEAYLKNAPE
                     MVRTMERSFGVKFRPVAKYPDYFPDKPGGKPGARTMEPVEFDASQLGEEFLRQREPYK
                     GTLVLGRVSMNQVEAHTLFGRAPGWLWLTLKMMLRYWLDFSWRRFTWRDRRLTLGQAL
                     AAQLRRALQQKNVPLTLNTAMDSLVEENGRVAGITATQNGNRHLRIRARKGVVLASGG
                     FESNQEMREEFLPQPSSSSWTAAPGCNTGDGIRAGRKLGAKLGFMNLVWGSPTVPVPG
                     ASAASALFVERSLPGCVMVNANGQRFVNEAAPYTEIVHAMYADHAKTGVSIPAWLIFD
                     ARFRKNYPVGPMLPGSMMPDSKLPKDWFGQVYFRADTLEQLCNDIGVNPDTLARTVQT
                     INNYASTGLDAEFGKGNNVFDRYYADPKIKPNPCLAPIVEAPFYAMPLTPGEIGTKGG
                     LVTDANARVLREDGSVIAGLYATGNCSAAVMGKTYAGPGATLGPAMTFAYIALRDIAG
                     AAQPSQVKAAA"
     gene            4878..5783
                     /locus_tag="VHE37_02945"
     CDS             4878..5783
                     /locus_tag="VHE37_02945"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HVT34514.1"
                     /translation="MGKTAILLVATLIAVPLLAYLDTPMSAFQWNMIGTVTLLMLCFA
                     LACFVTGEITGNVSQVDKLWSITPVIYAWVMAAKSGMDGRMLLMALVATVWGARLTYN
                     FSRHGGYSWKFWTGHEDYRWAEVRKMPEFSSPMAWKVFHFGFICLYQNALLLLITLPM
                     VKCASGASTLSGLDFLLALIFLALVALETWADQEQWNFQAEKKRRLAAGEPLTGRYES
                     GFIHDGLWAYSRHPNYLAEQSIWVVFYLFSVSAVGFNWTVAGCLLLILLFQGSSNMSE
                     GLQAKKYPRYADYQSRVPRFIPRFR"
     gene            5794..6546
                     /locus_tag="VHE37_02950"
     CDS             5794..6546
                     /locus_tag="VHE37_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT34515.1"
                     /translation="MKLKNRIALVTGAGQGVGQGIAYALAAEGAAVAVTGRTLAKLKD
                     TVAEIEKRGGRALAVECNVKDHASMRACVEKAVAHFGGLNILVNNAQEVPLGALNQVT
                     DEAYTAGWESGPLATFRLMKLCYPHLKGNGSIVNLGSPAAMRWDMSGYGAYAGVKEEI
                     RMLTRAAACEWAKDGIRTNVLLPLASSPAMAWWTRERPAEAAAFIATVPMQRVGDCEQ
                     DIGRFVATLCSDECRYINGQSIAADGGQAFLG"
     gene            6694..7899
                     /locus_tag="VHE37_02955"
     CDS             6694..7899
                     /locus_tag="VHE37_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HVT34516.1"
                     /translation="MAAPAFTPLRIGPLTLRNRFIKSATNEGMARGGVPSKALVEHHR
                     AIAAGGVGMTTVAYCAVSADGRTFVDQVTLDRASIPHLRALTDAVHAAGAAVCAQITH
                     GGAFNFLPQLSTRYPMSSSGGFNPPGVLSGRWFKHAMTADDMQRVSAEFVAGAQLARE
                     CGFDAVEIHMGHGYLLSQFISPLYNRRRDEYGGGAAARTRFPAEVLARVLDAVGGTTA
                     VSCKIGVTEGVKKGGTAADAVEVAKVLERTGAHLLVLSGGMNVESPWAIFNGRLPASA
                     TGSTQTPLLRFATRLSKLGEPDIQFRELYFLEHSRQVRAAVRMPLAYLGGAKSMDAVE
                     QALAEGFDAVAMGRALIHDAALVEKFRSGQTRVSGCTSCNQCVPMMYTEGGTRCVLQP
                     PRPGRPAAG"
     gene            8041..9342
                     /locus_tag="VHE37_02960"
     CDS             8041..9342
                     /locus_tag="VHE37_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34517.1"
                     /translation="MSNVSIVVAVKSCRSWLGAALLVAASAMSPAQAATVTVPVFDIS
                     ASAKYKDVAQPPKVAWVGDHEFVAVWGQLSPGSTDADGRWDLMMRRFNDSGAPLTGEI
                     RIANAHQLFAFDASMAIRAVDAGHYVVAWVDSDTSTGAGFDLYGQVFNLDGSAASLRF
                     PIDTVFDDNQVFPSIIPGPSGQFAVIYRSTDATSDGADIYAQRFSMAGRPVGSDVRIN
                     TVTSGLQSRPAADCDLKGHCLVVWNSSASADGSTPFEIKGQYVTPSLNMIGSEFLISN
                     DLPDSSSIPAVGMDGAQNAVVGWITFNSSTQHSNRVEFARYRPNHSFKNGNTLTADGV
                     EPDSTAFVGIMPTGEHGFFYSMENKTRSQLFVKDYTATGAIVSTSKLADVAKTSTLFV
                     GSSIAVNAAGRAVVTWQEYPASGSDTASYFRGAILQGFGTP"
     gene            complement(9403..9681)
                     /locus_tag="VHE37_02965"
     CDS             complement(9403..9681)
                     /locus_tag="VHE37_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34518.1"
                     /translation="MKKTKMLLLALTLCGTGVAYAAVDATMQADKQGIDAACTADAAT
                     AGCGSDQVGTGLKKCLAAWHKADPKNHHLSAGCETAMKAMHSDRKAGK"
     gene            9957..11120
                     /gene="otsB"
                     /locus_tag="VHE37_02970"
     CDS             9957..11120
                     /gene="otsB"
                     /locus_tag="VHE37_02970"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HVT34519.1"
                     /translation="MQPILDPEHAHLLGAYALPGTLIAFDYDGTLAPIVREPAHAHMR
                     PRTRELLTLLARRFPVAVISGRGRADVLRLLAGVPLVEVVGNHGAESLTALPDEIVAR
                     VRAWRAQLQPVLAQLPGSALEDKGWSLAIHYRGCADAAAAAQAVRTAASTLPRARLVP
                     GKAVLNVVSDDAPDKGAALLDLQRRAHCVRILFAGDDDTDDDVFRLQRAHPILGIQVG
                     ARATSLASYALRAQLEIDPLLERLLGADARAASGPAAAWPVALASGRAEARYRARTFV
                     TESTMSAPVYALNLFDVSSKEEYMAYSRRSAREVAAHGGRVVALGKFREVATGEIQPR
                     QVLILVEWQSKAAFDSYCNDPALADLHPHRVNGTRNYIWHLYDRLDDLRPLLK"
     gene            complement(11130..11585)
                     /locus_tag="VHE37_02975"
     CDS             complement(11130..11585)
                     /locus_tag="VHE37_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34520.1"
                     /translation="MPLSALDAAQVRGLLARHGLELQWVDQGAPIPGSYWGESEAGLI
                     GNVLYARADTPVHSLLHEACHYICMDEARRTQLHTNAGGADVEEHAVCYLQCVLADHL
                     PGYSRERCFADMDAWGYNFILGSAQAWFERDADDARAWLRRHRTHLGAA"
     gene            11679..>14878
                     /locus_tag="VHE37_02980"
     CDS             11679..>14878
                     /locus_tag="VHE37_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVT34521.1"
                     /translation="MDRIIKGAAAAALLALIASCHHSSPAASAGGNGGNSPPPIPAGD
                     NIFMAIVPPGSNGNSAGGIGAPAVIPTSDFVYPKNFRDQLDMYGDLAYAKTPLKADTC
                     APPADISQHVKSSDQACNYFKAAPLTITDAQAVSTRTLTAPNGKSVTIRRDGWGVPYI
                     EGDDRSSAEYGAGFAAAQDRLWLFDILRNVGRGRASAFLGPSPTTYDLDSEFGVPGGY
                     SEDELTTMVNNTVSKLGPLGPIFLNDTQMFVAGMNAYISYLQGAGVTELPPEYATLAA
                     GNLPKFPPAPFTVNDIVANAVLIQSALGRGGGGEATNLQLLQKLDPTFDSTKTQISQA
                     ACEMWRDLRHADIPDTPHTIDKAFPTQSPASLDETCPQTVPPGTAIWDVGSLQGRAFM
                     THAAGLPTGLPASSGGGSSATIPFSGMPSLGLPATRVASRNPGVKQKAKLTTLYASTD
                     PKSLKRMLNKYGVPMTTSNWIAVAASLTQSGHPIAVAGPQTGYDVPQLLWESAVVSHG
                     GTPLEIAARGISTVNLPYVVIGHGLDFAWSPTSAESDIQDTRVSKLCNTNGTPVTAAS
                     RNDADGDGFPDADGYVYKGQCVKLYKRVDSWTAYPTAASVALGGNPVPDNVKRYILRT
                     HYGPVTATATVGGAPVVISIQRSTFLSDVDTAAPFALLTTTGKPMNSVRFRQLFNSMS
                     SVFNWLYIDKNDIAYITSGLYPVRHAGINPDLPQWGDGNYEWAADEGFDPTQYGGDTP
                     FPARTVPVAQTADPLDGYFEWQGWLPLAAHIQEVNPPKGYMASWNNSGAPGWWAADSN
                     GTFGPTHRVQSLFLRLKAFVDSGRKFDIANMIEVMGDAAYTDLRGYDVLPQILALMKA
                     GTLTADQQQVVDLMQAWMDTGSRNWIDGGKGLGAFRRDRDASGTYDFRAQVVLMDAWY
                     AHMMDAVLPQMAALDAQGVSVLTGRYDAPRAQGSAYQEGWFQHMRRVFETVLETPGHQ
                     DYRSLKCAGTGVLADCRKAVLDSLDAALADLGGLSNQANWSGAASRYGDCPSSSIPGQ
                     SVSSCNVEDYDSVRHTSFSFLPVPAIHWINRPTFQQA"
BASE COUNT         2507 a         4913 c         4921 g         2537 t
ORIGIN      
        1 gccgttcggc tacgacggcc actactgcgc ggtggaccgg cgcgagaatc ccacggctgc
       61 ggcctgcgga tatttccagt cgggcatccg cgtgttcgac atccgcgacc cgatgcaccc
      121 ggcggagatc gcctacttca atccgccggc gcagtcggcc agcaaggcgc agctcaccgg
      181 ctcggaacac gccagcagcg ccgtgggcat cgacgccaac ctgagcacgg actggtgctc
      241 gtcgccgccg cgtttcgtcg cgcccgacca gctgtgggtc acctgccagg acaacggctt
      301 catggcgctg cgcttcacca atggcgcgtg gccgatcaag gacgcggtaa cgtcgccggc
      361 cgcggccacg gccggcgaca ccggccacta cggcggcgct gcgctgccgg gcctgtggct
      421 gccgctgctg ctggccgcgc tgcgacgcag gtttttggcc tcaccgcatg ccggttttcg
      481 atgttattaa ataatgaatt attttaaatt aatataataa gttataacaa tgaaaacggc
      541 cgtgcggttc aacaaaaaac ccgcgcagga gcgcgggttt tcttggcagc agcgttgcgc
      601 ttactggctg tcgaacgcgt ggcggatctc gggcttctgc ttgatgccct tgtcgatctc
      661 gatgaacttg cgcgccttga gttcggccgg caccttgtcg gcgtcgatgt agaactgctc
      721 gtaccactgg cggttctggt acaccgggcc gtcgccttcg cacaggatcg ggttgtcgat
      781 gcgcaccttg ttgtgccaga tttcgacgtc ttcgccgaag gctttattgg tcaggtcgac
      841 gtagctcttc accatcgcgt cgcactgttc ctgcgtcatg ccggggaatt tcttcaccag
      901 cacgccgaag cgcagctcga agctgttctc gttgatcggg gtgtgagaat tcaggagtat
      961 ggattcgacg ggcatgccgt tcatggcgcc gaacatgtgg gtgatctggt aggccgggcc
     1021 gtagtagctg gcgatggtcg tcaggaactc gtccttgccg cccagcttct cgttgctgcc
     1081 gaccatgacc tgggtggcct tgtggccttc gaacaggtcg gcgaaataca ccacgttgtc
     1141 cgagccgtgc accgagacga aatgcgccat gtcggcgatg ttgtcgatca gctcgcggca
     1201 gttgatgttg atggtccact tgaccagcga ccagtcggcc cagtcggacg agaacacggg
     1261 ggcgatgcgc ggaatggcct gctcaggtgc cggcgcgcgg ccttccgggt cgttccacac
     1321 gaacagcatc ttgttctctt cgcaggtcgg ccagctcttg acccgcgcct tgggcgggat
     1381 gcgctcgcag tacggaatgt ggttgcaggc gccatcgccg ccccagctcc agccgtggaa
     1441 cttgcagatc acggaatcgc cctgcacctt gcccagcgcg agatcggcgc ccatgtgcgg
     1501 gcaccaggcg tcgatcacgt tgagcttgcc gccctcgccc tggaagatga ccaggcgcgt
     1561 gccgaagatg ttgatgccgt gcggcttgcc gtccttgtac tcggcggcga ggcccaggca
     1621 atgccagccg cgcgcgtaac gcgcgggcgg cgccttggct tcgatcttgg cgatgggtga
     1681 ggcgctggtg gcggcgcgca tgacgacggc catgggatga ctcctgagtg ctggtgcggc
     1741 gagtggcggc taatgtggaa tcgcaccgcg cgcaacacat cacccatttg taggagcgaa
     1801 ccggtcacat ctctacgctc ctacgtttgg gtgatgcctg cgccgccgcg gcatgtgaaa
     1861 aagcgagccg caatagaacc agaggggaat cgcatgtccg cagtcacgcc gcagatgctg
     1921 gtccagcgcg cccgcgagat ggtgccggtg ctcaagcagc gcgcgaagca ggccgaggag
     1981 cagcgcaagg ttcccgacga aaccgtggcc gagatgcagg cggcgggttt gttccgcgtg
     2041 ctgcagccga agaagtacgg cggctacgag atggacccga aggtgttcta cgacgtgtgc
     2101 atgacgctgg ccgaaggctg catgagcacg gcgtggatct acggcgtgat cggcgtgcac
     2161 aactggcaga tcgcgctgtt cgatccgcgc gccgcggccg acgtgtggag caagggcacc
     2221 gacgtgcgca tcgcctccac ctacatgccc aagggccagg tgaaaccggt ggaaggcggc
     2281 ttccgcttct ccgggcgctg gggtttctcc agcggcattg cgcattgcga ctgggtgttc
     2341 ctcggcgggc tcatcttcaa ccagggccag ccgccggagt accgcacctt cctgctgccc
     2401 aaatccgact acgaagtggt cgacacctgg cacgtgatgg gcctgaaggg caccggctcg
     2461 aacgatatcg tggtcaagga cgtgttcgtg cctgagtacc gcacgcacaa ggccagcgac
     2521 ggcttcatgg gcaccaaccc gggccgtgac accttcacct ccgatctgta caagctgccg
     2581 ttcggccaga tcttcgtgcg cgcggtgtcc agcgcggcga tcggcgggct gcagggcgcg
     2641 ctggacacct tcctggattt cgccaaggtg cgcgtgggcg acatgggcaa caagaccagc
     2701 gaacaggcgc cggcgcagct ggcggcctcg gacgccgcaa tcgcggtgga cgagatgaag
     2761 ctggtgctga accgcaactt cgacgtgctg atggacaaga tccgcagaaa gcagccgctg
     2821 gacgacatca agcagcgcct gcacttccgc acgcaggcgg cgcaggtggt cgaccgctgt
     2881 gcgcgccatg cctaccagct gttctcggcc tgcggcgggc gcggcatctt caccgacttt
     2941 ccgctctacc gttacatgct cgacatctac gcggcgcgcg gacactacgc caacaacccg
     3001 gagcaattcg ggcgaaacta tggcggcgtg atgctgggac gcgacaacac ggacttcttc
     3061 atctgataaa aggtccgcaa cgggtgggga agtcatgaac gaagcaaaag gctgggatca
     3121 ggaatacgac gtggtcgtgg tcggctccgg cgcgggcggc atgaccgccg cgctgtgcgc
     3181 gcaggcgcag ggcctgtcgg cggtcgtggt ggaaaaggac aaggtctacg gcggcaccac
     3241 cgcggtgtcc ggcggcggca tctggattcc gtgcaacgac cagatggcgg cggccggcgt
     3301 gcccgacagc tacgaagaag cgctgaccta catgaagtac ctgaccggcg gcgaggtgcc
     3361 gctgtcgcgc atcgaggcgt acctgaagaa cgcaccggaa atggtgcgca ccatggaacg
     3421 cagcttcggc gtgaaattcc ggccggtggc gaaatacccg gattactttc ccgacaagcc
     3481 gggcggcaag ccgggcgcgc gcaccatgga gccggtggag ttcgacgcca gccagctggg
     3541 cgaggaattt ctccggcagc gcgagccgta caagggcacg ctggtgctcg gccgcgtgtc
     3601 gatgaaccag gtggaagcgc acaccctgtt cggccgtgcg ccgggctggc tgtggctcac
     3661 cctgaagatg atgctgcgct actggctgga tttttcctgg cgcaggttca cctggcgcga
     3721 ccgccggctc acgctgggcc aggcgctggc agcgcagctg cgccgcgcgc tgcagcagaa
     3781 aaacgtcccg ctgacgctga acacggccat ggactcgctg gtcgaggaga acggccgcgt
     3841 cgccggcatc accgccacgc agaacggcaa caggcacctg cgcatccgcg cgcgcaaggg
     3901 cgtggtgctg gccagcggcg gcttcgagtc caaccaggag atgcgcgagg aattcctgcc
     3961 gcagccttcg tcgagttctt ggaccgcggc gccgggctgc aacaccggcg acggcatccg
     4021 cgcagggcgc aagctcggcg ccaagctcgg cttcatgaac ctggtgtggg gttcgcccac
     4081 cgtgccggtg cccggcgctt cggcggcgtc cgcgctgttc gtcgagcgtt cgctgcccgg
     4141 ctgcgtgatg gtgaacgcca atggccagcg cttcgtcaac gaagcggcgc cgtacaccga
     4201 gatcgtgcac gccatgtatg ccgatcatgc caagaccggc gtcagcatcc cggcctggct
     4261 gatcttcgac gcgcgcttcc gcaagaacta cccggtaggg ccgatgctgc cgggcagcat
     4321 gatgccggac agcaagctgc ccaaggactg gttcggccag gtgtatttcc gcgccgatac
     4381 gctggagcag ctgtgcaacg acatcggggt caaccccgac acgctggcgc gcacggtgca
     4441 gaccatcaac aactacgcct cgaccgggct cgacgccgag ttcggcaagg gcaacaacgt
     4501 gttcgaccgc tactacgccg atcccaagat caagccgaac ccgtgcctgg cgccgatcgt
     4561 ggaggcgccg ttctacgcca tgcccctgac gccgggcgag atcggcacca agggtggcct
     4621 ggtcaccgac gcgaacgcgc gcgtgctgcg cgaggacggc agcgtgatcg ccggcctgta
     4681 cgccaccggc aactgctcgg ccgcggtgat gggcaagacc tacgccggcc cgggtgccac
     4741 gctcggcccg gccatgacct tcgcctacat cgcgctgcgc gacattgccg gcgccgcgca
     4801 gccgtcgcag gtcaaggccg cagcttaagc caccgcgttt ccacggccgc acggccactc
     4861 atttccacgg aaggcccatg ggcaagaccg cgatactgct cgtcgcaacg ctgatcgccg
     4921 tgccgctgct ggcgtacctg gatacgccga tgtcggcgtt ccagtggaac atgatcggca
     4981 cggtgacgct gctgatgctg tgcttcgcgc tggcctgctt cgtcaccggc gagatcaccg
     5041 gcaacgtgtc gcaggtggac aagctgtgga gcatcacgcc ggtgatctac gcctgggtca
     5101 tggcggccaa gtccggcatg gatggccgca tgctgctgat ggcgctggtg gccacagtgt
     5161 ggggcgcgcg gctcacctac aacttctcgc gccatggtgg ctacagctgg aaattctgga
     5221 ccggccacga ggattaccgc tgggccgagg ttcgcaagat gccggagttc agctcaccga
     5281 tggcatggaa ggtcttccac ttcggtttca tctgcctgta ccagaacgcg ctgctgctgc
     5341 tgatcaccct gcccatggtc aagtgcgcgt ccggcgcctc gaccttgtcc ggcctcgact
     5401 ttctgctggc gctgatcttc ctcgcactgg tcgcgctgga aacctgggcc gaccaggagc
     5461 agtggaactt ccaggcggag aagaagcgca ggctggccgc gggcgagccg ctgaccggcc
     5521 gctacgagag cggcttcatc cacgatggcc tgtgggccta ctcgcgccat ccaaactacc
     5581 tggccgagca atccatctgg gtagtgttct acctgttcag cgtgtcggcg gtcggtttta
     5641 actggaccgt ggccggctgc ctgctgctga tcctgctgtt ccagggcagt tcgaacatgt
     5701 ccgagggatt gcaggcaaaa aaatatccgc gctacgccga ctaccagtcg cgcgtgccgc
     5761 gctttatccc gcgcttccgc taggggtttt ccgatgaaac tgaaaaacag gatcgcgctg
     5821 gtcaccggcg ccggccaggg cgtgggccaa ggcatcgcgt atgcgctggc agcggaaggc
     5881 gctgccgtcg ccgtcaccgg ccgcactctg gccaagctga aggacaccgt ggccgagatc
     5941 gagaagcgtg gcggtcgcgc gctggcagtg gagtgcaacg tcaaggatca cgcatcgatg
     6001 cgcgcctgcg tcgagaaagc cgtcgcgcat ttcggtggcc tcaatatcct ggtcaacaac
     6061 gcgcaggaag tgccgctggg cgcgctcaat caggtgaccg acgaggccta caccgcgggt
     6121 tgggaatccg ggccgctcgc caccttccgc ctgatgaagc tgtgctatcc gcatctcaag
     6181 ggcaacggca gcatcgtcaa cctcggctcg cccgcggcga tgcgctggga catgagcggc
     6241 tacggcgcgt acgccggcgt caaggaagaa atccgcatgc tcacgcgcgc ggccgcctgc
     6301 gagtgggcga aagacggcat ccgcacgaac gtgttgttac cgctggcctc gtcgccggcc
     6361 atggcctggt ggacgcgcga gcgtccggcc gaggcggcgg cgttcatcgc caccgtgccc
     6421 atgcagcgcg tcggcgattg cgagcaggac atcggccgct tcgtcgccac cctgtgctcg
     6481 gacgagtgcc gctacatcaa tggccagagc atcgcggccg atggcgggca ggcgttcctc
     6541 ggctgaggaa cgcctgaaca agctgctgcg ctcggcattt tgcacgaggc cggtgctcgc
     6601 aatgctcaca tattttcaac atatgctccg cttgctgcgc tccgtcctcg cgcaatctgc
     6661 cttcgctcgc gacgcttctt caggtgttcc gtgatggccg cgccggcgtt cacgccgctg
     6721 cgcatcggcc cgctgacgct gcgcaaccgc ttcatcaagt ccgccaccaa cgagggcatg
     6781 gcgcgcggcg gcgtgccgtc gaaggcgctg gtcgagcacc atcgcgccat cgccgccggc
     6841 ggcgtgggca tgaccacggt ggcctactgc gcagtgagcg ccgacggccg cactttcgtc
     6901 gaccaggtca cgctggaccg tgccagcatc ccgcacctgc gcgcgttgac cgatgcggtg
     6961 catgccgccg gcgcggcggt atgcgcccag atcacgcatg gcggtgcgtt caatttcctg
     7021 ccgcagctca gcacgcgata cccgatgagt tcgtcgggcg gcttcaatcc gccgggcgtg
     7081 ctgtcgggcc gttggttcaa gcacgcgatg accgctgacg acatgcagcg cgtcagtgca
     7141 gagttcgtgg ccggcgcgca gctcgcgcgc gaatgcggct tcgacgccgt ggaaatccac
     7201 atgggccacg ggtacctgct gtcgcagttc atttcgccgc tgtacaaccg gcggcgcgat
     7261 gaatacggcg gaggcgcagc ggcgcgcacg cgctttcccg cggaggtcct ggcgcgcgtg
     7321 ctcgacgcgg taggcggcac caccgcggtg agctgcaaga tcggcgtgac cgagggtgtg
     7381 aagaaaggcg gcaccgccgc cgatgcggtg gaagtggcca aggtgctgga acgcaccggc
     7441 gcgcacctgc tggtgctgtc cggcggcatg aacgtcgaat cgccctgggc catcttcaac
     7501 ggcaggctgc cggccagcgc caccggcagc acgcagacgc cgctgctgcg ttttgccacg
     7561 cggctgtcca agctcggtga gccggacatc cagttccgcg aactgtattt tctagagcac
     7621 tcgcgccagg tgcgcgcggc agtgcgcatg ccgctggcct acctgggcgg ggccaagagc
     7681 atggacgcgg tggagcaggc gctggccgaa ggtttcgacg ccgtggccat gggccgcgcg
     7741 ctgatccatg acgcagcact ggtcgagaaa ttccgcagcg gccagacgcg cgtatcgggc
     7801 tgcacctcgt gcaaccagtg cgtgccgatg atgtacaccg agggcggcac gcgctgcgtg
     7861 ctgcagccgc cgcgaccggg gaggcctgct gccggctgat gcgcgtccgc atatttgcgc
     7921 gccgctgaca agaaaacttt cagaccattg cttgtgcgct gcggtttccc gtaaaaaacg
     7981 cgtccgctgc gccttcgggg gcgcgccgtt ttttttcttc cacgcaagca aggggaaacc
     8041 gtgtcgaacg tttcgatcgt tgttgccgtc aaatcctgcc gcagctggct cggggccgcg
     8101 ctgctggttg ccgcatccgc catgagtccc gcacaggccg ccaccgtgac ggtgccggtt
     8161 ttcgatattt cggccagcgc caagtacaag gacgtggcgc agccgcccaa ggtggcctgg
     8221 gtgggcgacc atgaattcgt cgccgtgtgg ggccagctga gcccgggctc gaccgatgcc
     8281 gacggccgct gggacctgat gatgcgtcgc ttcaacgaca gcggcgcgcc gctgaccggc
     8341 gagatccgca tcgccaatgc gcaccagctg ttcgcgttcg acgcctccat ggcgatccgc
     8401 gcggtcgacg ccggccacta cgtggtcgcc tgggtcgaca gcgacaccag caccggtgcg
     8461 ggcttcgatc tctacggcca ggtgttcaat ctcgacggca gcgcggccag cctgcgcttt
     8521 cccatcgata cggtgttcga cgacaaccag gtgttcccgt cgatcattcc cgggcccagc
     8581 ggccagttcg ccgtgatcta ccgctcgacc gatgccacct ccgacggcgc cgacatctat
     8641 gcgcaacgct tctccatggc cggccggccg gtgggcagcg acgtgcgcat caacaccgtc
     8701 accagcggac tgcagtcgcg cccggcggcg gattgcgatc tcaagggcca ttgcctggtg
     8761 gtgtggaaca gctcggcgtc cgccgatggc agcacgccgt tcgagatcaa gggacagtac
     8821 gtgacgccgt cgctgaacat gatcggcagc gagttcctca tctccaatga cctgcctgac
     8881 agcagcagca tcccggcggt gggcatggat ggtgcgcaga acgcggtggt cggctggatc
     8941 acgttcaatt cgtccacgca gcattccaac cgcgtggagt ttgcgcgcta ccggccgaac
     9001 cacagcttca agaacggcaa taccctgacc gccgacggcg tggagccgga cagcacagcc
     9061 ttcgtcggca tcatgcccac cggcgagcac ggcttcttct actcgatgga aaacaagact
     9121 cgctcgcagc tgttcgtcaa ggattacacc gccaccggcg ccatcgtttc caccagcaag
     9181 ctggccgatg tggccaagac ctcgacgctg ttcgtcggat ccagcatagc ggtcaatgcc
     9241 gcgggacgcg cggtggtgac gtggcaggag tacccggcgt cggggtcgga cacggcttcg
     9301 tatttccgcg gcgcgatcct gcaaggcttc ggcacgccct gacgcggctg cgcacgaaaa
     9361 aaagccccgg cattgccggg gcttttttgt tcaccgggaa gtttacttgc cggctttcct
     9421 gtcgctgtgc atcgccttca tcgcggtctc gcaaccggcg gagagatggt ggttctttgg
     9481 atccgctttg tgccaggcgg ccaggcactt cttcaggccg gtgccgacct ggtcgctgcc
     9541 gcagccggcg gtggcggcgt ccgcggtgca cgcggcatcg atgccctgct tgtcggcctg
     9601 catggtcgca tcgaccgcgg cgtaggcgac gccggttccg cacagcgtca gggccagcag
     9661 cagcattttc gtcttcttca tttccgatct cctggaggtt gatttgcagt ggccggcatg
     9721 cggccgggcg cgggtagcct aaccaagccg gcacaacgta aagagcagct ttacatttcg
     9781 cagcacttca ccgcctgtca tcatggaatg ttataaaaga taatactatt atttatttat
     9841 ttaatattta ataacatagg aaatgccctt gcggtggccg gtttcacggc ctgcaaatga
     9901 actgcccggg cgcctcctgc gtccgattag cgctggcacg gcatcccggc acgaccatgc
     9961 agccgatact cgatcctgaa cacgcacatc tgctgggcgc atacgcgctg cccggtacgc
    10021 tgatcgcttt cgattacgac ggcacgctgg cgccgatcgt gcgcgagccg gcgcatgcac
    10081 acatgcgccc gcgtacgcgc gagttgctga cgctgctggc gcgccgcttt cccgtggcgg
    10141 tgatcagcgg ccgcgggcgc gccgatgtgc tgcgcctcct ggccggcgtg ccgctggtgg
    10201 aggtggtcgg caatcacggc gccgagtcgc taacggcgct gccggacgaa atcgttgcgc
    10261 gggtgcgcgc ctggcgtgcg cagctgcagc cggtgctggc gcagctgccg ggcagtgcgc
    10321 tcgaggacaa gggctggtcg ctggcgatcc attatcgagg ctgcgccgat gcagcggcgg
    10381 cagcccaggc cgtgcgcacg gcggcgtcga cgctgccgcg cgcgcggctg gtgccgggca
    10441 aggcggtgct caacgtggtg tccgacgacg cgccggacaa gggcgcagcc ctgctcgacc
    10501 tgcagcgccg tgcgcactgc gtgcgcatcc tgttcgccgg agacgacgac accgacgacg
    10561 atgtgttccg gctgcagcgg gcgcatccca tcctcggcat ccaggtcggc gcgcgcgcga
    10621 cttcgctggc cagctacgcg ttgcgcgctc agctggagat cgacccgctg ctcgagcgcc
    10681 tgctgggtgc cgatgcacgg gctgcatccg gccctgccgc cgcctggccg gtcgcgctgg
    10741 catcgggtcg tgccgaagcg cgctatcgtg cgcgcacctt cgttacggaa agcaccatgt
    10801 ccgcacctgt ctatgcgctg aacctgttcg acgtctccag caaggaggaa tacatggcgt
    10861 actcgcgccg ctcggcgcgc gaagtcgccg cgcatggcgg gcgcgtcgtc gcgctgggca
    10921 agttccgcga ggtggccacc ggcgagatcc agccgcgcca ggtgctgatc ctggtggaat
    10981 ggcaatcgaa ggctgcgttc gacagctact gcaacgatcc cgcgctcgcc gacctgcatc
    11041 cgcaccgcgt caacggcacg cgcaactaca tctggcacct gtacgacagg ctcgacgacc
    11101 tgcggccgct gctgaagtag cgggctgcgc tatgccgcgc cgaggtgcgt acggtggcgg
    11161 cgcaaccagg cgcgcgcatc gtccgcatcg cgctcgaacc aggcctgcgc gctgccgagg
    11221 atgaagttgt agccccaggc gtccatgtcg gcgaagcagc gctcgcgcga gtagccgggc
    11281 aggtggtcgg ccagcacgca ttgcaggtag cacaccgcgt gctcctccac atcggcgccg
    11341 ccggcattgg tgtgcagctg cgtgcggcgg gcctcgtcca tgcagatgta gtgacaggct
    11401 tcgtgcagca gcgagtgcac cggcgtgtcc gctcgcgcgt aaagcacgtt gccgatcagc
    11461 ccggcttcgg actcgcccca gtagctgccg ggaatcggcg cgccttggtc cacccattgc
    11521 agctcgagtc catgccgggc cagcaggccg cgcacctgcg ccgcatcgag ggccgacagt
    11581 ggcaccacgg ctgattcgat cgagtgagac atgcgcggcg ggcaggggga tactatgggg
    11641 aacgccgcag accagacgac ggcgcccaga ggagagtcat ggacaggatt ataaaaggcg
    11701 ctgccgcggc ggcgctgctc gcgctcattg cttcgtgcca tcacagcagc cccgcggctt
    11761 ccgcgggcgg caacggcggc aactcgccgc cgccgattcc cgccggcgac aacatcttca
    11821 tggccatcgt gccgccgggc tccaacggca attcggccgg cggcatcggt gcgccggcgg
    11881 tgattccgac cagcgatttc gtctatccga agaatttccg cgaccagctc gacatgtacg
    11941 gtgacctggc ctatgccaag acgccgctca aggccgacac ctgcgcgccg cctgccgaca
    12001 tcagccagca cgtgaagagt tccgaccagg cgtgcaacta cttcaaggcc gcgccactga
    12061 ccatcaccga tgcgcaggca gtgtcgacgc gcacgctgac cgcgcccaac ggcaagagcg
    12121 tgaccatccg ccgcgacggc tggggcgtgc cctacatcga gggtgacgat cgttcttccg
    12181 ccgagtacgg cgcgggtttc gccgcggcgc aggatcgcct ttggctgttc gacatcctgc
    12241 gcaatgtcgg ccgcgggcgg gcgtcggcat ttctcggccc ttcgccgacc acctacgacc
    12301 tcgacagcga gtttggcgtg cccggcggct acagcgagga cgagctgacc accatggtca
    12361 acaacacggt gagcaagctc ggcccgctcg gcccgatctt cctcaacgac acgcagatgt
    12421 tcgtcgccgg catgaacgcg tacatcagct acctgcaggg cgcgggcgtc accgaactgc
    12481 cgccggaata cgcgacgctt gcggcgggca acctgcccaa gtttccaccg gcgccgttca
    12541 cggtgaacga catcgtggcc aacgcggtgc tgatccagtc ggcgctcggc cgcggcggcg
    12601 gcggtgaagc gaccaacctg cagctgctgc agaaactcga ccccacattc gattcaacca
    12661 agacacagat ttcgcaggcg gcctgcgaga tgtggcgcga cctgcgccat gccgacattc
    12721 ccgacacgcc gcacaccatc gacaaggctt tcccgacgca gtctcccgct tcgctggacg
    12781 aaacctgtcc gcagaccgtg ccgccgggca cggccatctg ggacgtgggc agcctgcagg
    12841 gtcgcgcgtt catgacgcac gctgccggtc tgccgacggg gttaccggct tcctcaggtg
    12901 gcggctcgtc cgccacgatc ccgttttccg gcatgccctc gctgggcctg cctgcgaccc
    12961 gcgttgcgtc gcgcaatccg ggagtgaagc agaaggcgaa gctgacgacg ctctacgcct
    13021 ccactgatcc caagagcctc aagcgcatgc tcaacaagta tggcgtgccg atgaccacct
    13081 cgaactggat cgcggtggcg gcgagcctga cccagagcgg ccatccgatc gccgtggccg
    13141 gtccgcaaac cggctatgac gtgccgcagc tgctgtggga atcggctgtc gtttcgcacg
    13201 gcggcacgcc gctggaaatc gccgcgcgcg gcatctccac cgtgaacctg ccgtacgtgg
    13261 tgattggcca tggactggat ttcgcctgga gcccgacctc ggccgaatcc gacatccagg
    13321 acacgcgcgt atcgaagctg tgcaacacca atggcacgcc ggtcacggcc gcttcacgca
    13381 acgatgccga tggcgacggg tttcccgatg ccgatggcta tgtctacaag ggccagtgcg
    13441 tgaagttgta caagcgcgtc gacagctgga cggcgtatcc gaccgcggcc agcgtggcgc
    13501 tgggcggcaa cccggtgccg gacaacgtca agcgttacat cctgcgcacg cactatggcc
    13561 cggtcaccgc gaccgcgacc gtcggcggcg cgccagtggt gatctcgatc cagcgcagca
    13621 ccttcctgtc cgacgtcgac actgccgctc cgttcgcgct gttgacgacc acgggcaagc
    13681 cgatgaacag cgtgcgcttc cgccagctgt tcaacagcat gagcagcgtg ttcaactggc
    13741 tgtacatcga caagaacgac atcgcctaca tcacgtctgg cctgtacccg gtgcgccacg
    13801 ccggcatcaa cccggacctg ccgcaatggg gtgatggcaa ttacgagtgg gcggccgacg
    13861 agggcttcga tccgacacag tatggcggcg acacgccgtt ccccgcgcgc accgtgccgg
    13921 tggcgcagac cgccgatccg ctcgacggct acttcgaatg gcagggctgg ttgccgctgg
    13981 ccgcgcatat ccaggaagtg aatccgccca agggttatat ggccagctgg aacaacagcg
    14041 gcgcgcccgg ctggtgggcg gccgacagca acggcacctt cggcccgacg caccgcgtgc
    14101 agagcctgtt cctgcggctc aaggccttcg tcgacagcgg gcgcaagttc gacatcgcca
    14161 acatgatcga ggtgatgggc gacgccgcct acaccgacct gcgtggctac gacgtgctgc
    14221 cgcagatcct cgcgctgatg aaagccggca cgctcaccgc cgaccagcag caggtggttg
    14281 acctgatgca ggcgtggatg gacaccggct cgcgcaactg gatcgacggc ggcaagggcc
    14341 tgggtgcgtt ccggcgcgac cgcgacgcca gcggcaccta tgacttccgg gcgcaggtgg
    14401 tgctgatgga tgcctggtac gcgcacatga tggacgcggt gctgccgcag atggcggcgc
    14461 tggatgcgca gggtgtttcg gtgctcactg gccgctacga cgcgccgcgc gcgcagggct
    14521 cggcctacca ggagggctgg ttccagcaca tgcgccgcgt gttcgagacg gtgctggaga
    14581 cgccggggca ccaggactac cgctcgctca agtgcgcggg caccggcgtg ctggccgact
    14641 gccgcaaggc ggtgctcgat tcgctcgacg cggcgctggc cgacctcggt gggctgtcga
    14701 accaggccaa ctggagcggc gcggcttcgc gctatggcga ctgcccgtcg tcatccatcc
    14761 cgggccagtc ggtgagcagc tgcaacgtcg aggactacga ctccgtgcgc cataccagct
    14821 tctcgttcct gccggtgccg gcgatccact ggatcaaccg ccccaccttc cagcaggc
//
LOCUS       DASZZC010000126         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_267952,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000126 DASZZC010000000
VERSION     DASZZC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267952"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VHE37_02985"
     CDS             complement(<1..1009)
                     /locus_tag="VHE37_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT34522.1"
                     /translation="MTQAFRLASGGTIDRSRPLRFTFDGREYTGYAGDTLASALLANG
                     VRITGRSFKYHRARGIYGAGYEEPNTLVQLGSGARTEPNLKATQVQLADGLEARAVNC
                     WPSARFDVLAPLRWFKAFMPSGFYYKTFMLPGWHWFEGIIRAAAGLGRSPREADPDRY
                     DKRFESVDVLVVGGGTSGVEAAQRAAASGKQVLLVDDQPQVQGIAASSNLRLRTRCTV
                     FGYYDHDYLLAIEQLAPGPVRQRLWHIRARQVILATGAIERPLVFPHNDRPGVMLASA
                     AHQYFSRYAVLPGRRLVIATNNDSAYATALELLIAGAQIVAVLDARGSSVSPEAQALA
                     AR"
     gene            complement(1006..1296)
                     /locus_tag="VHE37_02990"
     CDS             complement(1006..1296)
                     /locus_tag="VHE37_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HVT34523.1"
                     /translation="MIKFDCPFCGPRDEQEFRCGGQSHITRPGPHDQVSDQAWAEYLY
                     LRENPKGWHFERWQHVAGCRQWFNVARHTATHEIRAVYAMTAPRPQLEGAGP"
     gene            complement(1296..>2491)
                     /locus_tag="VHE37_02995"
     CDS             complement(1296..>2491)
                     /locus_tag="VHE37_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HVT34524.1"
                     /translation="RHWQPAWRNAALKPSYDVVIVGGGGHGLATAYYLARNHGVRNVA
                     VIEKGWLGGGNTGRNTTVVRSNYFFPQSAAFYELALKLYEGLSRELNYNIMLSQRGMM
                     NLAHSRHQVEVARRILNGMRLNGIDAEWLEPDQVHAREPLLRLNGRYPVLGALLQPRG
                     GTARHDAVAWGYARAADALGVDIIQCCEVTGFLRSGNAVTGVRTSRGDVNAGRVCLSV
                     AGHTSQLAALAGLRLPVTSYALQAFVSEPVKPVLNSVTLSPSTGVYISQTDKGEVVVG
                     GALDLYPSYAQRGNFHTVENVTAGMLEMFPMFSRLRLMRQWAGIVDIVKDSSPILGPS
                     PVDNLYLNCGFGTGGFKAIPAGGFTMAHTLATGKRHPLIEDFGLERFTSGALIDEAGA
                     SGIAH"
BASE COUNT          391 a          835 c          869 g          396 t
ORIGIN      
        1 cgcgcgccgc cagagcctgc gcttcgggtg agaccgacga gccgcgcgca tccagcaccg
       61 cgacgatctg cgcaccggcg atcagcagct cgagcgcggt ggcatacgcg ctgtcgttgt
      121 tggtggcgat gaccagcctg cgtcccggca gtactgcata acgcgaaaaa tactggtgcg
      181 cggcgctggc cagcatcacg cccggccggt cgttgtgcgg gaataccagc ggccgctcga
      241 tggcgccggt ggccaggatc acctgccgcg cgcggatatg ccacagccgc tggcgcaccg
      301 gtccgggggc cagctgctcg atggccagca ggtagtcgtg atcgtaatag ccgaacacgg
      361 tgcagcgtgt gcgcaggcgc aggttggaag acgccgcgat gccctgcacc tgcggctggt
      421 cgtcgaccag caatacctgc ttgcccgacg cggccgcgcg ctgcgccgcc tccacgccgg
      481 aagttccgcc gcccaccacc agcacatcga ccgattcaaa acgcttgtcg taacggtccg
      541 ggtcggcctc gcgcgggctg cggcccaggc cggcggcggc gcggatgatg ccttcaaacc
      601 aatgccagcc cggcagcatg aaggtcttgt agtagaagcc cgacggcatg aatgccttga
      661 accagcgcag cggcgccagc acgtcgaagc gcgcgctggg ccagcagttc accgcacgcg
      721 cttccaggcc atcggccagt tggacctgcg tcgccttgag attgggctcg gtgcgcgcac
      781 cgctgcccag ctgcaccagg gtgttcggct cttcgtatcc cgcgccgtag atgccgcgcg
      841 cgcggtggta cttgaagctg cggccggtga tgcgcacgcc attggccagc agtgcggatg
      901 cgagggtgtc gccggcgtag ccggtgtatt cgcggccgtc gaaggtgaag cgcagcgggc
      961 ggctgcggtc gatggtgccg ccggaagcga ggcggaaagc ttgcgtcacg ggcccgctcc
     1021 ttcaagctgc ggtcgcggcg ccgtcatggc gtacaccgcg cggatctcgt gtgtcgccgt
     1081 gtggcgcgcg acgttgaacc actgccggca gccggccacg tgctgccagc gctcgaaatg
     1141 ccagcccttg gggttctcgc gcaggtacag gtactcggcc caggcctggt cgctgacctg
     1201 gtcgtgcgga ccgggccgcg tgatgtggct ctggccgccg cagcggaatt cctgctcgtc
     1261 gcgcggaccg cagaatgggc agtcgaactt gatcatcagt gtgcgatccc cgacgcgccg
     1321 gcttcgtcga tcagtgcccc gctggtgaag cgttcgaggc cgaaatcctc gatcagcggg
     1381 tggcgcttgc cggtggccag cgtatgcgcc atggtgaagc cgccggccgg gatggccttg
     1441 aaaccgccgg tgccgaagcc gcagttgaga taaaggttgt cgaccgggct cgggccgagg
     1501 atgggcgacg agtccttgac gatgtcgacg atgccggccc actgccgcat caggcgcagg
     1561 cgcgagaaca tcgggaacat ctccagcatg ccggcggtga cgttctctac cgtgtggaaa
     1621 ttgccgcgct gcgcgtacga cgggtacagg tcaagcgcgc cgccgaccac cacctcgccc
     1681 ttgtcggtct ggctgatgta gacgccggtg gacggcgaca gcgtcaccga gttgagcacc
     1741 ggcttgaccg gctcggacac gaacgcctgc agcgcatagc tggtcaccgg caggcgcagg
     1801 cctgccagcg ccgccagctg gctggtgtgg ccggcaaccg acaggcacac gcggccggcg
     1861 ttgacgtcgc cgcgcgaggt gcgcacgccg gtcaccgcgt tgccgctgcg caggaagccg
     1921 gtgacttcgc agcactggat gatatccacg cccagcgcgt cggccgcgcg cgcgtaaccc
     1981 caggccaccg cgtcatggcg cgcggtgccg ccgcgcggct gcagcagcgc gccgagcacc
     2041 gggtagcggc cattgagccg cagcagcggt tcgcgcgcgt gcacctggtc cggctctagc
     2101 cattccgcgt cgatgccgtt gaggcgcatg ccattgagga tgcggcgcgc gacttccacc
     2161 tggtggcgcg agtgcgccag gttcatcatt ccgcgctgcg acagcatgat gttgtagttg
     2221 agttcgcggc tcagcccttc gtagagcttg agcgccagct cgtagaacgc agcggattgc
     2281 ggaaagaaat aattcgagcg caccaccgtg gtgttacggc cggtgttgcc gccgcccagc
     2341 cagcccttct cgatcaccgc cacgttgcgc acgccgtggt tgcgcgccag gtaataagcg
     2401 gtggccaggc catggccacc gccgccgacg atgaccacgt catagctcgg cttgagcgcg
     2461 gcattgcgcc atgccggctg ccaatggcga t
//
LOCUS       DASZZC010000127         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_392536,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000127 DASZZC010000000
VERSION     DASZZC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392536"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..528
                     /locus_tag="VHE37_03000"
     CDS             79..528
                     /locus_tag="VHE37_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVT34525.1"
                     /translation="MSASARKNDVMTRWVSFRLDGQLYGLAIGEVQEVLASADIEPVP
                     GAPPFVLGVINLRGHIVTVLDLRSCLGTRTAADGASCLVIANSGSQLLGFCVDGIADV
                     VNIPQARIQPPLELSTMPAARAVRGLIKRKDEVLTLLDVGQLVPAAA"
     gene            716..1018
                     /locus_tag="VHE37_03005"
     CDS             716..1018
                     /locus_tag="VHE37_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT34526.1"
                     /translation="MHTQTSTPDAAVIRLPAELAIEDAAALREQLLTHVDDETPVTLE
                     AADITRIHTAALQQLYLFCRDRRAAGRSVHWSRVSPALKSAADLAGVTRMLAPGQE"
     gene            1025..1387
                     /locus_tag="VHE37_03010"
     CDS             1025..1387
                     /locus_tag="VHE37_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34527.1"
                     /translation="MPHILAVDDSASMRQMVCFTLKSAGYEVTEAEDGAVALGLAQKQ
                     KFNLVLADVNMPNMDGLSLVKALRGLPDYKFTPLLMLTTESTPEKKQEGKAAGATGWL
                     VKPFNPDQLIGTIKRVLG"
     gene            1413..2606
                     /locus_tag="VHE37_03015"
     CDS             1413..2606
                     /locus_tag="VHE37_03015"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34528.1"
                     /translation="MIDILFVDDDVPALESLQQHLADAQDTLRVRVAAGGKEALLALQ
                     AEPADVVVADLSMPEMDGAQLLRCVRDLHPRTMRVLLATPGERAQALAAMAAAHQVLV
                     KPVAPASLRKSITRVYELQNKLHSPGVLHAISKQSSLPAVPKLYWQLAREVDGSTATS
                     ASVSAILQQDPSMTARMLQVVNSAVFSLARNITHVQDAVTYLGFEPVRSLVLAMELFR
                     AMSALCAPQGFSLERIQNHSLRVGRIAMNLVSSADDKKTAFSAAMLHDLGKMILAMAM
                     PEEYAVVQKHAQQHGLAEYEAESDIYGCCHAEVGAHLLALWGLPHALVEAVAFHHTPA
                     SLTDEHFGIAGAVHVADWLEHHLYTPTGVQAADDGLDHDYLRNVGVLDRLPEWLGQAQ
                     KRLAA"
BASE COUNT          492 a          986 c          956 g          447 t
ORIGIN      
        1 gggagaaggc gacgcgccgg cgcccgatgc tacgaattcc gaagcaacgc cggcgccgcc
       61 ggccggagcg ccgcagccat gagcgccagc gcacgcaaga acgacgtgat gacccgctgg
      121 gtcagtttcc gcctggacgg ccagctctac ggcctggcca tcggcgaggt gcaggaagtg
      181 ctggccagcg ccgacatcga gccggtgccc ggcgcgccgc cgttcgtgct cggcgtgatc
      241 aacctgcgcg ggcacatcgt caccgtgctc gacctgcgca gctgcctggg cacgcgcacc
      301 gcggcagacg gcgccagctg cctggtcatc gccaacagcg gcagccagct gctcggcttc
      361 tgcgtcgacg gcatcgccga cgtggtcaac atcccgcagg cgcgcatcca gccaccgctg
      421 gagctgtcca ccatgccggc ggcgcgcgcg gtgcgcgggc tgatcaagcg caaggacgaa
      481 gtgctgaccc tgctcgacgt cggccagctg gtgccggcgg cggcctgaaa accaccgcac
      541 cgcagggtcg ttttgcttgt tattaattat ttgacgttta ttaaaacaat aaataattaa
      601 taacatggcc ggaggcggtg cggtggcagg tttttcaggc ccgcgcgtca agcgcacggc
      661 gcgcggcacg gaataaaagt tgcctgcgct gcgccaggct catcgaggca ttgccatgca
      721 cacccagaca tccacgcctg acgccgccgt catccgcctt cccgccgagc tggcgatcga
      781 ggacgccgct gcattgcgtg agcagctgct cacgcatgtc gacgacgaga ctccagttac
      841 gctggaagct gccgatatca cgcgcataca caccgcggct ttgcagcagc tgtatctgtt
      901 ttgccgtgac cgccgcgccg ccgggcgcag cgtgcactgg tcgcgggttt cgccggcgtt
      961 gaagagcgca gccgacctgg ccggagtgac caggatgctc gcgcccgggc aggagtagta
     1021 cgcgatgcca cacatccttg cggtcgacga ttccgcctcc atgcgccaga tggtgtgctt
     1081 cacgctcaag tccgccggct acgaggtgac cgaggccgag gatggcgcgg tggcgctggg
     1141 cctggcgcag aagcagaaat tcaacctggt gctggccgac gtcaacatgc cgaacatgga
     1201 cggcctgtcg ctggtgaagg cgctgcgcgg cctgcccgac tacaagttca cgccgctgct
     1261 catgctcacc accgagtcca cgccggagaa gaagcaggaa ggcaaggccg ccggcgccac
     1321 cggctggctg gtcaagccgt tcaacccgga tcagctgatc ggcaccatca agcgcgtgct
     1381 cggctgagca cgcccaacca gcggagttgc ccatgatcga catcctgttc gtcgacgacg
     1441 acgtgccggc gctggaatca ctccagcagc acctggccga cgcgcaggac acgttgcgcg
     1501 tgcgcgtggc ggccggcggc aaggaagcgc tgctggcgct gcaggcggaa ccggccgacg
     1561 tggtggtggc cgacctgtcg atgccggaaa tggatggcgc gcagctgctg cgctgcgtgc
     1621 gcgacctgca cccgcgcacc atgcgcgtgc tgctggccac gccgggcgag cgcgcgcagg
     1681 cgctggcggc gatggccgcc gcgcaccagg tgctggtgaa gccggtggcg ccggcctcgc
     1741 tgcgcaaatc gatcacccgc gtgtacgagc tgcagaacaa gctgcacagc cccggcgtgc
     1801 tgcatgccat cagcaagcag agcagcctgc cggcggtgcc caagctgtac tggcagctgg
     1861 cgcgcgaggt cgacggcagc accgccacct ccgcctcggt cagcgccatc ctgcagcagg
     1921 acccgtcgat gaccgcgcgc atgctgcagg tggtgaactc cgcggtgttc tcgctggcgc
     1981 gcaacatcac ccacgtgcag gacgcggtga cctacctcgg cttcgagccg gtgcgctcgc
     2041 tggtgctggc catggaactg ttccgcgcca tgtcggcgct gtgcgcgccg cagggcttct
     2101 cgctcgagcg catccagaac cattcgctgc gcgtgggccg catcgccatg aacctggtga
     2161 gctcggccga cgacaagaag accgcgttct ccgccgccat gctgcatgac ctgggcaaga
     2221 tgatcctcgc catggccatg ccggaggaat acgcggtggt gcagaagcac gcgcagcagc
     2281 acggcctggc agagtacgaa gccgagtccg acatctacgg ctgctgccac gccgaggtgg
     2341 gcgcgcacct gctggcgctg tggggcctgc cgcacgcgct ggtcgaggcg gtggccttcc
     2401 accacacgcc ggccagtctc accgacgaac atttcggcat cgccggcgcg gtgcacgtgg
     2461 cggactggct cgagcaccac ctgtacacgc ccaccggtgt ccaggccgcg gacgacgggc
     2521 tggatcacga ttacctgcgc aacgtcggcg tgctcgaccg cctgccggag tggctgggcc
     2581 aggcgcagaa gcggctcgcc gcgtgagacg cgcatctgcg cgcccacaag ccgccgagca
     2641 aacacaccgc tgtcctgtgc atcaatcgcc tctgcgcggt gaatgcgggc gcttcgcgcg
     2701 ctcgtgctgc aggtcattct gcgagagaaa agacgccgct gaagctctgc gctgacggct
     2761 gattcacgcc acgcaggaat cccgtcgtcc gtcaggtttt tcctgacacg ccgtcagaaa
     2821 tgtcctgatc ggtctttggc tgcgttttgc aaaccatacg ctccacgcga tgcgctccaa
     2881 c
//
LOCUS       DASZZC010000128         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1231304,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000128 DASZZC010000000
VERSION     DASZZC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231304"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VHE37_03020"
     CDS             <1..947
                     /locus_tag="VHE37_03020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT34529.1"
                     /translation="PRAIHQPFALAGASITLRPAGHILGAAGVLVQDDSSLYFSGDLG
                     RQHDPLMRAPEPPPACDHLVIESTYGDRLHPPGDPFQQLGEVINRVARRGGVVVIPAF
                     AVGRTQSLLYGLLRLKQAQAIPDDLPVYLNSPMAIDVTGLYRRHPAEHALGAAQCAAM
                     AHVARLVNTADESRHLNTLKGPMVIIAGSGMATGGRVVHHLQAFASDARNAIVLAGFQ
                     AGGTRGAALAAGAESIKIHGAYVAVRADVVVLNQLSAHADQGELLAWLAQAPSPPRRV
                     FVTHGEPSAADTLRRCIEERLHLAACVPEFGQDEVLAR"
     gene            992..1261
                     /locus_tag="VHE37_03025"
     CDS             992..1261
                     /locus_tag="VHE37_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34530.1"
                     /translation="MTTRNARPSLARQRTLLRARLQKLRVQLADSSSRSTRAQVTAAE
                     RELQRIENVIARGIAASAGLCEHCQRPRGDCSRCSLMQSQRRTRP"
     gene            1258..>1647
                     /locus_tag="VHE37_03030"
     CDS             1258..>1647
                     /locus_tag="VHE37_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34531.1"
                     /translation="MNDASIWQPQPELLLPRKRLKLKLLSYNIHVGLNTGHYTHYLTR
                     AWRHALPGADMHRVLDPIADLMRDYDFVAIQEGDAGSLRTWFRNQIEYLAHRAGFAHW
                     GLAVTRDLRPLAMHCLGFLSRLPPAAVE"
BASE COUNT          241 a          622 c          550 g          234 t
ORIGIN      
        1 agccgcgcgc catccatcaa ccatttgcgc tggccggcgc cagcatcacg ctgcgccccg
       61 ccggccacat cctgggcgcc gccggcgtgc tggtgcagga tgacagttcg ctctacttca
      121 gcggcgatct cggccgccag cacgatccgc tgatgcgcgc gccggaaccg ccgccggcct
      181 gcgatcacct ggtgatcgag tccacctacg gcgatcggct gcatccgccg ggcgatccgt
      241 tccagcaact gggcgaggtg atcaaccgcg tggcgcgccg cggcggcgtg gtggtgatcc
      301 cggcgtttgc cgtcggccgc acccagtcgc tgctgtatgg cctgttgcgc ttgaagcagg
      361 cacaagccat tcccgatgac ctgccggtct acctgaacag cccgatggcg atcgacgtca
      421 ccgggctcta tcgccggcac ccggcggaac atgcgctggg cgccgcgcaa tgcgcggcca
      481 tggcgcacgt tgcgcggctg gtgaacaccg ccgacgagtc gcgccacctc aacacgttga
      541 agggtccgat ggtgatcatc gccggcagcg gcatggccac cggcgggcgc gtggtgcatc
      601 atctgcaggc cttcgcttcc gacgcccgca acgccatcgt gctggccggg ttccaggcgg
      661 gcggcacgcg cggtgcggcg ctggcggccg gcgccgagtc gatcaagatc cacggtgcct
      721 acgttgccgt gcgcgccgac gtggtcgtgc tcaaccagct gtcggcgcac gccgaccagg
      781 gcgaactgct ggcctggctg gcgcaggcac cgtcgccgcc gcgccgcgtg ttcgtcaccc
      841 acggcgagcc gtcggcggcc gacaccctgc gccgctgcat cgaggaacgc ctgcacctgg
      901 cggcctgcgt gccggaattc ggccaggacg aagtgctggc ccgctgaccc aggtcaatgc
      961 cgccccggcg cgcgctgcgc acagtgcggg catgaccacg cgcaatgccc gacccagcct
     1021 ggcccgccag cgcacgctgc tgcgcgcgcg cctgcagaag ctgcgcgtgc agctggcgga
     1081 cagctcgtcg cgcagcacgc gcgcgcaggt caccgcggca gagcgcgagc tgcagcgcat
     1141 cgagaacgtc atcgcgcgcg gcatcgctgc ctcggccggc ctgtgtgaac actgccaacg
     1201 gccgcgcggc gactgcagcc gctgcagcct gatgcagagc cagcggcgca cgcggccatg
     1261 aacgatgctt ccatctggca gccgcagccc gaactgctgc tgccgcgcaa gcgcctcaag
     1321 ctgaagctgc tcagctacaa catccacgtg ggcctcaaca ccggccacta cacgcactac
     1381 ctgacgcgcg cctggcgcca cgcgttgccg ggcgccgaca tgcaccgcgt gctcgatccc
     1441 atcgccgacc tcatgcgcga ctacgacttc gtcgccatcc aggaaggcga tgccggcagc
     1501 ctgcgcacct ggttccgcaa ccagatcgaa tacctcgcgc accgcgccgg ctttgcgcac
     1561 tggggcctgg cggtgacgcg cgacctgcgc ccgctggcca tgcactgcct gggtttcctc
     1621 agccggctgc cgcccgcggc ggtggag
//
LOCUS       DASZZC010000129         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_718262,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000129 DASZZC010000000
VERSION     DASZZC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718262"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VHE37_03035"
     CDS             <1..727
                     /locus_tag="VHE37_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT34532.1"
                     /translation="TLRAAQLAALAGRDYDAVRKLRDEINRVAPTPQDERAWTDIALK
                     QNPKVLAQQMAVEVAGYQSKRASGRRFPRIDVVGGYWHSNADGGIPFRGNEDLSRIGA
                     RVTLPLFTGGQISSAVREADEMEQSAQQLLEDAKRQAVLDTRQAFLSCNDGLQKVPAL
                     RHALEASIAQIDAVTGGYEAGTRSYADVLAAVQQRYAEESAWAAARYDFLLQTLKLKA
                     ASGTLVPDDLARLNALLESAASP"
     gene            735..3125
                     /locus_tag="VHE37_03040"
     CDS             735..3125
                     /locus_tag="VHE37_03040"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVT34533.1"
                     /translation="MVMPAPDPRPQRLHLQTRYRVFAPAQLIERHGSWEARSSRPNHL
                     PLQLDAKTVALAQRFDGQRSTADIARTLAHDGETLSARQLEGIAAELGQSGLLFPGTH
                     EPLPVPAYTESEMQQLGWSGGEAGLAGRPMDAGPLPPSTVPGSRAYPGLVDAPGGLID
                     RGAEDHRRRWDLPSATFVRVGALFGWSYRSRTGAVLAAAMAAGMIAALEAHRLELART
                     AYDAWESTLWIVLLPLAAWLVNFVACAARAHAIATYTPERAHCVLQLGFLGVPVFAVE
                     TRGVIERAGLAARLRVIGAPVAALLALAAGMALAWMVTVRTDPRLAQFCATVAAVAWV
                     AWILRANPLVKRDGYLLLTHRIGVLDLREQASAALFGTRRPWAHQLRVLPRPWLVAYA
                     ILSVTFLTGMMAVMFSHAAPLLSAHFGGIGFLMAVGFSGAYMYRHIKEALTSPRATLG
                     RGFDWLEWRPSRTEWILIGCAIVLLMFPYRYEPSGDMVVLPHERADVRALVPGQVREV
                     LVAEGDAVKKDQVIARMDDTEARARVAQAQSALAQAEADLALLKKGAKNEEVEVARQR
                     VATARKKADVSRENAQRVAQAFAKKGVTAWENDRARGQADVDAQEVLDAQRQLDLITS
                     AAVDERITAKQAEVEGARATLEFNKKQLADTEIKAPIAGSIASGSLKFALGNFLQRGE
                     LLAQVEDTSQLIAEIKLPESSIGEIHPGNPASAKAWAYPGTSFDGTVTSVAPSAENAP
                     YGKVVRVQMSLQDPDNRLKPEMTGSAKASGDRHLLIVVYTRALMRFIAIEVWSWLP"
     gene            3113..>3403
                     /locus_tag="VHE37_03045"
     CDS             3113..>3403
                     /locus_tag="VHE37_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34534.1"
                     /translation="MAPLDSVPGTAGRALHERDFRLTCDSGFGDGWNHYAHSMAWFEG
                     KLYVGTTRGSTVALKTNDPPPDWSPWPVDCPNDPYDIDRRAQIWQYTPASGAW"
BASE COUNT          519 a         1153 c         1251 g          480 t
ORIGIN      
        1 cacgctgcgc gccgcgcagc tggcggcgct ggccgggcgc gactacgacg cggtgcgcaa
       61 gctgcgcgac gagatcaacc gggtggcgcc gacgccgcag gacgagcgcg cctggaccga
      121 catcgcgctc aagcagaatc cgaaagtact ggcgcagcag atggcggtgg aagtggccgg
      181 ctaccagagc aagcgcgcca gcggccggcg ctttccgcgc atcgacgtgg tcggcggcta
      241 ctggcacagc aacgccgacg gcggcatccc gttccgcggc aacgaagatc tctcgcgcat
      301 cggtgcgcgg gtgacgctgc cgctgttcac cggcgggcag atcagctcgg cggtgcgcga
      361 ggccgacgag atggagcaga gcgcgcagca gctgctggag gacgcgaagc gccaggcggt
      421 gctcgacacg cgccaggcct tcctcagctg caacgatggc ctgcagaaag tgccggcgct
      481 gcgccatgcg ctggaagcgt caatcgcgca gatcgacgcc gtgaccggcg gctacgaagc
      541 cggcacgcgc agctatgccg acgtgctcgc cgccgtgcag cagcgctacg cggaggaaag
      601 cgcgtgggcc gccgcgcgct acgacttcct gctgcagacg ctgaagctca aggccgcttc
      661 gggcacgctg gtgccggacg acctggcgcg gctcaacgct ctgctggaga gcgccgcgtc
      721 gccttgagat cagcatggtg atgcccgctc ccgatccgcg gccgcagcgg ctgcacctgc
      781 agacgcgtta ccgcgtgttt gcgccggcgc agctgatcga gcgccacggc agctgggaag
      841 cgcgcagcag ccggcccaac cacctgccgc tgcagctcga cgcgaaaacc gtggcgctgg
      901 cgcagcgctt cgacggccag cgctccaccg cggacattgc gcgcacgctg gcgcacgacg
      961 gcgaaacgct gagcgcgcgc cagctggaag gcatcgccgc ggaactgggc cagtccggcc
     1021 tgctgtttcc cggcacgcat gaaccgctgc cggtaccggc gtacaccgaa tcagagatgc
     1081 agcagctcgg ctggtccggc ggcgaagcgg gcctcgccgg ccggccgatg gacgccggcc
     1141 cgctgccgcc gtccaccgtg ccgggttcgc gcgcgtatcc cgggctggtc gatgcgccgg
     1201 gcgggttgat cgatcgcggt gccgaagacc accgccgccg ctgggatctg ccgtcggcga
     1261 cgttcgtgcg cgtgggcgcc ttgttcggct ggtcgtaccg ctcgcgcact ggcgccgtac
     1321 tggccgcggc catggccgcc ggcatgatcg ccgcgctgga agcgcaccgg ctggagctgg
     1381 cgcgcaccgc ctacgacgcc tgggaatcca ccctgtggat cgtgctgctg ccgctggcgg
     1441 catggctggt gaacttcgtc gcctgcgcgg cgcgcgcgca tgccatcgcc acgtacacgc
     1501 cggaacgcgc gcactgcgtg ctccagctgg gcttcctcgg cgtgccggtg ttcgcggtgg
     1561 agacgcgcgg cgtgatcgag cgcgccggct tggccgcgcg gctgcgcgtg atcggcgcgc
     1621 cggtggcggc gctgctggcg ctggccgccg gcatggcgct ggcgtggatg gtgacggtgc
     1681 gcaccgatcc gcggctggcg cagttctgcg ccaccgttgc ggcggtggcc tgggtggcgt
     1741 ggatcctgcg cgccaacccg ctggtcaagc gcgacggcta cctgctgttg acgcaccgca
     1801 ttggcgtgct cgacctgcgc gagcaggcct cggccgcgct gttcggcacg cggcggccat
     1861 gggcgcacca gctgcgcgtg ctgccgcggc cgtggctggt ggcgtatgcc atcctctcgg
     1921 tgacctttct caccggcatg atggcggtga tgttcagcca tgcggcgccg ctgctcagcg
     1981 cgcatttcgg cggcatcgga tttctgatgg cggtgggttt ttccggagcg tacatgtaca
     2041 ggcatatcaa ggaagcgctg acttcgccgc gggccacgct gggccgcggc ttcgactggc
     2101 tggagtggcg gccgtcgcgc accgagtgga ttctcatcgg ctgcgccatc gtgctgctga
     2161 tgtttcccta ccgctacgag ccctcgggcg acatggtggt gctgccgcac gaacgcgccg
     2221 acgtgcgcgc gctggtgccc ggccaggtgc gcgaggtgct ggtggccgaa ggcgacgcgg
     2281 tgaagaagga ccaggtgatc gcgcgcatgg acgacaccga ggcgcgcgcg cgcgtggcgc
     2341 aggcgcagtc ggcgctggcg caggccgagg cggatctcgc gctgctgaag aagggcgcga
     2401 agaatgagga agtcgaagtc gcgcgccagc gcgtggccac ggcgaggaag aaggccgacg
     2461 tctcgcgcga gaacgcgcag cgcgtggcgc aggcctttgc caagaagggc gtcaccgcct
     2521 gggagaacga ccgcgcgcgc ggccaggccg acgtcgacgc gcaggaagtg ctcgacgcgc
     2581 agcgccagct ggacctgatc accagcgccg cggtggacga gcggatcacc gccaagcagg
     2641 cggaagtgga gggcgcgcgc gccacgctgg aattcaacaa gaagcagctg gccgacaccg
     2701 agatcaaggc gccgattgcc ggcagcatcg cctccggctc gctcaagttc gcgctgggca
     2761 atttcctgca gcgcggcgaa ctgctggcac aggtggagga caccagccag ctgatcgccg
     2821 agatcaagct gccggaatcg tccatcggcg aaattcatcc cggcaacccg gccagcgcca
     2881 aggcatgggc gtatcccggc acctcgttcg acggcacggt gacctcggtg gcgccgtcgg
     2941 cggagaacgc gccctacgga aaagtggtgc gcgtgcagat gagcctgcag gacccggaca
     3001 accggctcaa gccggagatg accggcagcg ccaaggcctc gggcgaccgc cacctgctga
     3061 tcgtggtgta cacgcgcgcg ctgatgcgct tcatcgccat cgaagtgtgg tcgtggctcc
     3121 cctagattcg gttccgggca ccgcgggtcg cgcgctgcac gagcgtgatt tccgcctgac
     3181 ctgcgacagc ggtttcggcg acggctggaa ccactacgcg cacagcatgg cctggttcga
     3241 gggcaagctt tacgtgggca ccacgcgtgg cagcacggtg gcgctgaaga ccaacgatcc
     3301 gccgccggac tggagcccgt ggccggtgga ctgcccgaac gatccgtacg acatcgaccg
     3361 ccgcgcgcag atctggcagt acacgccggc gagcggcgct tgg
//
LOCUS       DASZZC010000130         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_268496,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000130 DASZZC010000000
VERSION     DASZZC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268496"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VHE37_03050"
     CDS             complement(<1..583)
                     /locus_tag="VHE37_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HVT34535.1"
                     /translation="MSIVIDRRLNDRNKNAVNRQRFLRRYKDQIKKSVSDIVSKRGIT
                     DMNQGGVVNVPIKDISEPSFRHGKGGDHEYVNAGNREFHTGDRIPRPRGQGGGGGGSG
                     EGDGEETQDNFAFALSREEFMNLFFDDLELPHLIRNTLGDVQEYKWQRAGYTPTGVPS
                     NLSVTRSLRQAMARRIALTGGLKKELAELEQAVE"
     gene            complement(610..2532)
                     /locus_tag="VHE37_03055"
     CDS             complement(610..2532)
                     /locus_tag="VHE37_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HVT34536.1"
                     /translation="MSVLRKFQENYLKARDEELTLEEYLTLCKRDRMAYASPAERMLA
                     AIGEPEVIDTRNDPRLSRIFSNRIIRRYPAFAEFFGLEDVIAQIVAFFKHAAQGLEER
                     KQVLYLLGPVGGGKSSIAERLKSLMETHPIYALKGSPVNESPLGLFSPERDGEMLEKE
                     YGIARRYLTGIPSPWAVKRLAEFEGDLSKFKVVRIQPSVLKQTAITKTEPGDENNQDI
                     SSLVGKVDIRKLETYAQDDPDAYSYSGGLCLGNQGLLEFVEMFKAPIKMLHPLLTATQ
                     EGNFKGTEGFSAIPFQGIILAHSNESEWQTFRNNKNNEAFLDRVYIVKVPYCLQWSEE
                     VKIYEKLLRNSSLAEAKCAPGVLDMMAQFSVLTRLKEPENSSIYSKMRVYDGENLKDT
                     DPAAKSLQEYRDYAGIDEGMTGISTRFAYKVLSAVFNFDQTEVAANPVHLMYVLEQRI
                     LREQLPEETQRRYLEFIKGWLAPRYAEFIGKEIQTAYLESYSEYGQNIFDRYVTFADY
                     WIQDEDYRDQETGESFDRAALNAELEKIEKPAGIANPKDFRNEIVNFVLRARATNNGK
                     NPAWTSYEKLREVIEKKMFSSTEDLLPVISFNAKASAEDKEKHENFVGRMVDKGYTEK
                     QVRLLSEWYLRVRKSQ"
     gene            2773..>4178
                     /locus_tag="VHE37_03060"
     CDS             2773..>4178
                     /locus_tag="VHE37_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HVT34537.1"
                     /translation="MPTHALDSLPLDNRYARLPVDYHSHVAPTPLPAPRLLHFNEEAA
                     RLIDLDPREAQRPEFAEIFGGNRPLPGGDPVSMLYAGHQFGVWVPQLGDGRAILLGQV
                     RNSRGDSWDLQLKGAGPTPYSRHADGRAVLRSSVREYLCGEAMHHLGIPTTRALCVVT
                     SPAPVRRETIETAAVICRMAPSHVRFGNFEVFYYRNQHALLKPLADHVIAEHYPQHAG
                     DYAAWLGEVVERTARLMAMWQAVGFCHGVMNTDNMSVLGITLDYGPYGFMDGFEAGHI
                     CNHSDDGGRYAYDQQPMIGHWNCSRLVQACLPLLHETPERAVEIGSAILDRYPPAYAK
                     EMMARWLSKLGLREERDGDVPLVNRWLHLLDSGRSDFTRSFRALAQVGTRADAPAPAL
                     RDEMVNLAAFDAWLAQYRERLRAEHSDDADRAARMNQVNPKYVLRNHLAQAAIDAAQQ
                     GSAAEIDRLMQLLRRPYD"
BASE COUNT          663 a         1391 c         1327 g          797 t
ORIGIN      
        1 cttccactgc ctgttccagc tcggccagct ccttcttcag tccgccggtc agtgcgatgc
       61 gccgcgccat ggcctggcgc agcgagcgcg tcaccgacag gttgctcggc acgccggtgg
      121 gcgtgtagcc ggcacgctgc cacttgtatt cctgcacgtc gcccagcgtg ttgcggatca
      181 ggtgcggcag ctcgaggtcg tcgaagaaca ggttcatgaa ctcttcgcgc gacagggcaa
      241 aggcgaagtt gtcctgcgtc tcctcgccgt cgccctcgcc gctgccgccg ccaccgccgc
      301 cctgcccgcg cggccgcggg atgcggtcgc cggtgtggaa ttcgcggttg ccggcgttga
      361 cgtactcgtg gtcgccgccc ttgccgtggc ggaagctcgg ctccgagatg tccttgatcg
      421 gcacgttgac cacgccgccc tggttcatgt cggtgatgcc gcgcttggac acgatgtccg
      481 agacggattt cttgatctgg tccttgtagc gccgcaggaa acgctggcgg ttgaccgcgt
      541 tcttgttgcg gtcgttgagg cggcggtcga tgacgatgga catcgagagg tttgtcgcgc
      601 tccttcagtt cactgcgact tgcgcaccct caggtaccac tcggacagca ggcgcacctg
      661 cttctcggtg tagcccttgt cgaccatgcg gccgacaaag ttctcgtgct tctccttgtc
      721 ctcggcggag gccttggcgt taaacgagat caccggcagc agatcctcgg tgctgctgaa
      781 catcttcttc tcgatcacct cccgcagctt ctcgtagctg gtccaggccg ggttcttgcc
      841 gttgttggtg gcgcgcgcgc gcagcacgaa gttgacgatt tcgttgcgga agtccttggg
      901 gttggcgatg cccgccggct tctcgatctt ctccagctcg gcgttgagcg ccgcgcggtc
      961 gaaactctcg ccggtctcct ggtcgcggta atcctcgtcc tggatccagt agtcggcgaa
     1021 ggtgacgtag cggtcgaaga tgttctgccc gtactcggaa tagctttcca ggtacgcggt
     1081 ctggatttcc ttgccgatga actcggcgta gcgcggcgcc agccagccct tgatgaactc
     1141 gaggtagcgg cgctgggttt cctcgggcag ctgctcgcgc aggatgcgct gctccagcac
     1201 gtacatcagg tgcaccggat tggccgccac ctcggtctgg tcgaagttga acaccgccga
     1261 caagaccttg taggcgaagc gcgtggaaat gccggtcatg ccttcatcga tgcccgcgta
     1321 gtcgcggtac tcctgcaggc tcttggccgc cgggtcggtg tccttcaggt tctcgccgtc
     1381 gtacacgcgc atcttcgagt agatgctgct gttctccggt tccttcaggc gcgtgagcac
     1441 ggagaactgc gccatcatgt cgagtacgcc gggcgcgcac ttggcctcag ccagcgagct
     1501 gttgcgcagc agcttctcgt agatcttcac ctcctccgac cactgcaggc agtacggcac
     1561 cttgacgatg tagacgcggt cgagaaacgc ctcgttgttc ttgttgttgc ggaacgtctg
     1621 ccactcggac tcgttcgagt gcgccaggat gatgccctgg aacggaatgg cgctgaaccc
     1681 ttcggtgccc ttgaagttgc cttcctgcgt cgccgtcagc agcgggtgca gcatcttgat
     1741 cggcgccttg aacatctcga cgaactcgag caggccctgg ttgcccaggc acaggccgcc
     1801 ggaataggaa tacgcgtcgg gatcatcctg cgcataggtt tccagcttgc ggatgtccac
     1861 cttgccgacc agcgaggaga tgtcctggtt gttctcgtcg cccggctcgg tcttggtgat
     1921 ggcggtctgc ttgagcactg acggctggat gcgcaccacc ttgaacttcg acaggtcgcc
     1981 ctcgaattcg gccaggcgtt tcaccgccca tggcgaggga atgccggtga ggtaacgccg
     2041 cgcaatgccg tattcctttt ccagcatctc gccatcgcgc tccggcgaga acagacccag
     2101 cggcgattcg ttcaccggcg agcccttcag cgcgtagatc gggtgcgtct ccatcaggct
     2161 cttcagccgc tcggcgatgg acgacttgcc gccgccgacc gggcccagca ggtagagcac
     2221 ctgcttgcgc tcctccagcc cctgcgcggc atgcttgaag aaggccacga tctgcgcaat
     2281 gacgtcttcc aggccgaaga attcggcgaa agccgggtag cggcggatga tgcggttgga
     2341 aaatatgcga gacaggcgcg gatcgttgcg cgtgtcgatc acttcgggct cgccgatggc
     2401 ggcgagcatg cgttcggccg gcgaggcata agccatgcgg tcgcgcttgc acagggtgag
     2461 gtactcctcc agggtcagtt cctcgtcgcg cgccttcaga taattttcct gaaactttct
     2521 gagtacgctc atcgtcccga cctcctgtgc acggttgacg gaaaccgacc caagcatacg
     2581 ccgttccgcc tgtgtcttcc gacagtgcaa aaggcggacc gtttcagttt ttctgatgaa
     2641 cggagcatca gctcagcccc ggctgaatcc ggtgcagacc aacggccgca ccaacgccac
     2701 gaacggcaag cagtccttcg ttaagactgc caatcggcgc aaaaaatccg gcgcatgttc
     2761 cacaatgacg gaatgccgac gcatgccctc gactcgctgc cgctggacaa ccgctacgcc
     2821 cgcctcccgg ttgactacca ctcgcatgtg gcgcccacgc cattgcccgc gccgcgcctg
     2881 ctgcacttca acgaagaagc ggcgcgactg atcgacctgg acccgcgcga ggcgcagcgg
     2941 ccggagtttg cggaaatctt cggcggcaac cgcccgctgc ccggtggcga tccggtctcg
     3001 atgctctacg ccggccacca gttcggcgtg tgggtgccgc agctgggcga cggccgcgcc
     3061 attttgctcg gccaggtgcg caacagccgc ggcgacagct gggacctgca gctcaagggc
     3121 gcggggccga cgccctactc gcgccacgcc gacggccgcg cggtgctgcg ctcgtcggtg
     3181 cgcgaatacc tgtgcggcga ggccatgcac cacctcggca ttcccaccac ccgcgcgctg
     3241 tgcgtcgtca cctcgcccgc gccggtgcgg cgcgagacca tcgagaccgc cgcggtgatc
     3301 tgccgcatgg cgccgtcgca cgtgcgcttc ggcaacttcg aggtgttcta ctaccgcaac
     3361 cagcacgcgc tgctcaagcc gctggccgat catgtgatcg cggaacacta cccgcagcac
     3421 gccggcgatt acgccgcctg gctgggcgag gtcgtcgagc gcaccgcgcg gctgatggcc
     3481 atgtggcagg cggtcggctt ctgccacggc gtgatgaaca ccgacaacat gagcgtgctc
     3541 ggcatcacgc tggactacgg cccgtatggc ttcatggatg gtttcgaggc cggccacatc
     3601 tgcaaccact ccgacgatgg cggccgctac gcctacgacc agcagccgat gatcggccac
     3661 tggaactgct cgcgcctggt ccaggcctgc ctgccgctgc tgcacgagac gccggagcgc
     3721 gcggtggaaa tcggcagcgc gattctcgac cgctacccgc ctgcgtatgc caaggaaatg
     3781 atggcgcgct ggctgtccaa gctcggcctg cgcgaggaac gcgacggcga cgtgccgctg
     3841 gtcaaccgct ggctgcacct gctcgacagc gggcgcagcg acttcacccg cagcttccgc
     3901 gcgctggcgc aggtgggcac gcgcgccgat gcgccggcgc ccgcgctgcg cgatgagatg
     3961 gtcaacctgg ccgcgttcga tgcctggctc gcgcagtacc gcgagcgcct gcgtgccgag
     4021 cattcggacg acgcggaccg cgccgcgcgc atgaaccagg tcaatccgaa atacgtgctg
     4081 cgcaatcacc tggcgcaggc ggccatcgac gctgcgcagc agggcagcgc tgcggaaatc
     4141 gaccggctga tgcagctgct gcggcggcca tacgacga
//
LOCUS       DASZZC010000131         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_718569,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000131 DASZZC010000000
VERSION     DASZZC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718569"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3111)
                     /locus_tag="VHE37_03065"
     CDS             complement(<1..>3111)
                     /locus_tag="VHE37_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HVT34538.1"
                     /translation="PESLFILLTSDSGRAMLGGVRSVIVDELHALAGNKRGSHLMLSL
                     ERLSALTGREPVRIGLSATQKPIEDMARYLMGARKPCAIVDTGYTRQRDIAMEVPRSP
                     LDAVMSNEVWTEIYDKLAELTRQHRTTLVFVNQRRLAERAARHLAERIGEEHVTAHHG
                     SLAKEHRLKAEQRLKSGQLKALVATSALELGIDIGDVDLVCQLGSPRSIAAFLQRVGR
                     AGHAVGAIPKGRLFPLSLDELLEGAALIDAVNRGELDRIRVPAQPLDVLAQQIVAECG
                     SREWRADELYARCTQAAPYRELKREDFDKILAMLADGYATRRGRRGAYLHWDQVNGVL
                     RGRRGARLTALTNAGVIPDQFDYDVVLLPEEHRVGTLNEDFSFESLPGDIFQLGNTSY
                     RIVKIEVGKVYVEDARGQPPTLPFWLGEGLGRSDELSHSVSRLTARAEELIAQDLTAC
                     ARWLEQEVTLPAAGAQQLTQYLATAHAALDLLPSEKRIVLERFFDEVGDAHLVIHSPY
                     GSRINRAWGLALRKRFCRSFNFELQASALEDSIVLSLGPTHSFPLDDIRHYLKSSSVR
                     EVLVQALLDAPMFGTRWRWVASTALALKRNINGKRLPPQFQRSDSEDLLTVVFPDQVA
                     CAENLTGERQIPDHPLVVQTIRDCLHEVMDIDGLERVLAGIERGEIEMIGRDLTGPSP
                     LSNAILNAKPYAFLDDGEAEERRTRAVSAQRLHDLDSAASLGRLDPAAIARVREEAAP
                     DFGNADELHDALMVYGFLTTEEVFKSSDPHPDPLPVEEGAKQNASPSGRGRGAAAGEG
                     LAATLARDLVEQRRATQIDGRIWIAAERLHEFLALAPQARLSAPLKPRHEIPDAHDAL
                     REIVRSRLELSGPISARELAAPLGVGAQQTEAALLQLEAEGGVMRGAFDGSGDTQWCE
                     RRLLARIHRYTRERQRASVSAVTPAQFMRFLLRWHQVEGAGGGDERRQGEAGLYAVLQ
                     QLEGCAASASAWERDLLPARVRYYAPDMLDKLCAAGRIAWLRPVSGGATAGGGIDEGG
                     RKGGPIR"
BASE COUNT          441 a         1076 c         1100 g          494 t
ORIGIN      
        1 cggatggggc cgcccttacg gcctccttcg tcgatgccgc cgccggcagt cgctcctccc
       61 gaaacgggac gcaaccacgc gatgcgcccg gcggcgcaca gcttgtcgag catgtcgggc
      121 gcgtagtagc gcacgcgcgc gggcagcagg tcacgctccc acgcgctggc cgatgcggcg
      181 cagccttcca gctgctgcag caccgcgtag aggccggctt cgccctggcg gcgctcgtcg
      241 ccgccgccgg cgccttccac ctggtgccag cgcagcagga agcgcatgaa ctgcgccggc
      301 gtcaccgcgc tgacgctggc gcgctggcgc tcgcgcgtgt agcggtggat gcgcgcgagc
      361 aggcgccgct cgcaccactg cgtgtcgcca ctgccgtcga aagcgccgcg catcacgccg
      421 ccctcggctt ccagctgcag cagcgcggcc tccgtctgct gcgcgcccac gcccagcggc
      481 gcggcgagct cgcgcgcgga gatcggcccg gacagttcca ggcgcgagcg cacgatctcg
      541 cgcagcgcgt cgtgcgcgtc gggaatttca tgccgcggct tgagtggcgc ggacaggcgc
      601 gcctgcggcg ctagcgcaag gaattcatgc aggcgttcgg ccgcgatcca gatgcggcca
      661 tcgatctgcg tagcgcggcg ctgttcgacc agatcgcgtg cgagcgttgc agcaagtccc
      721 tcacccgcgg ctgcgccgcg gcctctcccg gagggagagg cattctgctt tgctccctct
      781 tccaccggga gcgggtcggg gtgagggtca ctgcttttga aaacttcctc ggtcgtcagg
      841 aagccataca ccatcagcgc gtcgtgcagt tcgtcggcgt tgccgaaatc cggcgcggct
      901 tcttcgcgca cgcgcgcgat ggcggcggga tcgagccggc cgagactggc cgcggaatcc
      961 agatcgtgca gccgctgggc cgacaccgcg cgcgtgcggc gctcctcggc ttcgccgtcg
     1021 tcgaggaacg cgtaaggctt ggcgttgagg atcgcgttcg acagcggcga gggtccggtg
     1081 agatcgcggc cgatcatctc gatctcgccg cgctcgatgc cggccagcac gcgctccagc
     1141 ccgtcgatgt ccatcacctc gtgcaggcag tcgcggatgg tctgtacgac gagcggatga
     1201 tcgggaatct gccgctcgcc ggtcaggttc tcggcgcagg ccacctggtc ggggaacacc
     1261 acggtgagca ggtcttccga atcgctgcgc tggaactgcg gcggcaaccg cttgccattg
     1321 atgttccgct tgagcgccag cgcagtgctg gcgacccagc gccagcgcgt gccgaacatc
     1381 ggcgcgtcga gcagcgcctg caccagcacc tcgcgcacgc tggacgattt caggtagtgc
     1441 ctgatgtcgt cgagcgggaa gctgtgcgtg gggccgagcg agagcacgat gctgtcttcc
     1501 agcgcggatg cctgcagctc gaagttgaag ctgcggcaga agcgcttgcg cagcgccagg
     1561 ccccaggcgc ggttgatgcg cgagccgtag ggcgagtgga tcaccaggtg cgcgtcgccc
     1621 acttcgtcga agaagcgctc gagcacgatg cgcttttcgc tcggcaggag gtcgagcgcg
     1681 gcgtgggcgg tggccaggta ctgcgtcagc tgctgcgcgc cggcagccgg cagcgtcact
     1741 tcctgctcca gccagcgcgc acaggcggtc aggtcctgcg cgatcagctc ctccgcgcgc
     1801 gcggtgaggc gcgacaccga gtgcgacagc tcgtcgctgc ggcccaggcc ttcgccgagc
     1861 cagaacggca gcgtcggcgg ctggcccctg gcgtcttcca cgtacacctt gccgacctcg
     1921 atcttgacga tgcggtagct ggtgttgccc agctggaaga tgtcgccggg caggctctcg
     1981 aaggagaaat cctcgttgag cgtgccgacg cggtgctcct ccggcaacag caccacgtcg
     2041 taatcgaact ggtccgggat gacgccggca ttggtcagcg cggtgaggcg cgcgccgcgc
     2101 cgcccgcgca gcacgccgtt gacctggtcc cagtgcaggt aggcgccgcg ccgcccgcgc
     2161 cgcgtggcgt agccatcggc cagcatggcg aggatcttgt cgaagtcctc gcgtttcaat
     2221 tcgcggtacg gggccgcctg cgtgcagcgc gcgtacagct cgtccgcgcg ccactcgcgc
     2281 gagccgcact cggcgacgat ctgctgcgcc agcacgtcga gcggctgcgc cggcacgcgg
     2341 atgcggtcga gctcgccgcg gttgaccgcg tcgatcagcg ccgcgccttc cagcagttca
     2401 tccagcgaca gcgggaacag gcgtcctttc gggatggcgc cgacggcgtg accggcgcgg
     2461 cccacgcgct gcaggaaggc ggcgatcgag cgcggcgaac cgagctggca caccaagtcg
     2521 acgtcgccga tgtcgatgcc gagttctaga gccgatgtcg ccaccagcgc tttcagctgg
     2581 ccactcttca gccgctgctc ggctttcagg cggtgctcct tggccaggct gccatgatgc
     2641 gcggtgacat gctcctcgcc gatgcgctcg gccaggtggc gcgcggcgcg ctcggccagg
     2701 cggcgctggt tgacgaaaac cagcgtggtg cggtgctggc gcgtgagctc ggccagcttg
     2761 tcgtagatct cggtccacac ttcgttggac atcaccgcgt cgagcggcga gcgcggcact
     2821 tccatggcga tgtcgcgctg gcgcgtgtag ccggtgtcga cgatggcgca aggcttgcgc
     2881 gcgcccatca ggtagcgcgc catgtcttcg atgggcttct gcgtggccga cagcccgatg
     2941 cgcaccggct cgcgcccggt cagcgccgac agccgttcca gtgacagcat caggtgcgac
     3001 ccgcgcttgt tgccggccag cgcgtgcagt tcgtcgacga tcacgctgcg cacgccgccg
     3061 agcatggcgc ggccggagtc cgaagtgagc aggatgaaca gcgattccgg c
//
LOCUS       DASZZC010000132         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_75364,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000132 DASZZC010000000
VERSION     DASZZC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75364"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VHE37_03070"
     CDS             complement(<1..537)
                     /locus_tag="VHE37_03070"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HVT34539.1"
                     /translation="MNKLQDTIESAWNARDTLDKNNPAIRDAVTQAIDLLDKGEARVA
                     EPAASGWQVNQWLKKAVLLYFRLHDNAPIQMGALSGFDKVPLKYAGWTAVDFAKQGAR
                     VVPPAAVRKGAFISPGAILMPSYVNIGAYVGKNTMVDTWATVGSCAQIGKHVHLSGGV
                     GIGGVLEPPTASPVIIEDG"
     gene            complement(749..3430)
                     /gene="glnD"
                     /locus_tag="VHE37_03075"
     CDS             complement(749..3430)
                     /gene="glnD"
                     /locus_tag="VHE37_03075"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HVT34540.1"
                     /translation="MGAEFDLAEDEELGIFSAQKVRSRLAQGGYAIAAFRDTLKWGRE
                     RLYGLFHDGTPADSLVHARAHLVDEILRAAWQKFLPDNPPGLTMVAVGGYGRGELLPH
                     SDIDILLLHDGDALERNRPRLEQLTAFGWDIGLEVGQSVRTPDECAQEAAKDITVMTN
                     MMEARLLTGDTRLYAQMQASITPDHVWPVKEFFEAKRNEQRIRYRKYDDTGYKLEPNV
                     KESPGGLRDIHTIGWVAKRHFGAQTLGELRDRGFLTKQECDELFAGQDFLWRVRFALH
                     MITGRREDRLLFDHQIKIAAVFGYEDNDKNRAVEQFMQLYYRTIKSLSCLNDMLLQLF
                     EEAILRPDEASTAEPLNARFAVCGGYIEARSDDVFQRQPQALLEIFLLLQQHPKIGGI
                     RAQTLRLILRDSRYLTEAVREDVRSRALFIEMFRQGRGLTRNLRRMNRYGVLGRYLPA
                     FGKIVGLMQYDLFHTLTVDEHTLYVVRNARRFAMARFRDELPHCSEVMDRLAKPELLY
                     LAAFFHDMAKGRGGDHSELGAHEAEEFCLKHGLSHPDAELVAWLVKNHLVMSLTAQKK
                     DVSDPQVVADFALKVGDRARLDYIYLLTCADIRATNPALWNSWRESLLKQLYDSTARA
                     LERGLNNPVLEGEMVSEKRARALALLAARNIEAAAVERVWSRFDHDYFLRYSPEELAW
                     HAPEIARVPEGELPLVLVESAFGRGTTVFVYTRDRDHLFGLSTGVLARLGLNILDARL
                     HTTRDGYVLDSYAVTESDGSAIANPLRFEEIRTQLRKVLSDPDVSTIDVSRRSSQRLR
                     HFSTPTMVYFGQDAARNRTILELVTADQPGLLSLIGRVFQKRGILLDAAKIGTIGERA
                     EDVFYITDRMHRPIVSEDALDDLREVITRTLNRSEAA"
     gene            complement(3439..4203)
                     /gene="map"
                     /locus_tag="VHE37_03080"
     CDS             complement(3439..4203)
                     /gene="map"
                     /locus_tag="VHE37_03080"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVT34541.1"
                     /translation="MSITLKNPEEQQQMRAAGAAAAQVLQMIGPHVKPGVTTELLNQI
                     CHNYIVDELRCTPAPLNYKGFPKSICTSVNHVVCHGIPGDKVLKRGDVINIDITVIKD
                     GFHGDTSKMFFVGEPTVLARRLVQTAHEAMIAGIRQVKAGARLGDIGHAIQSLTEARG
                     YSVVREYCGHGIGRIFHEEPQVLHYGKPGTGLELKAGMTITVEPMINAGRPDVRLLPD
                     GWTVVTKDHSLSAQWEHTVLVTETGYDVLTLRDGEM"
     gene            4408..5253
                     /gene="rpsB"
                     /locus_tag="VHE37_03085"
     CDS             4408..5253
                     /gene="rpsB"
                     /locus_tag="VHE37_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HVT34542.1"
                     /translation="MATITMRQLLEAGVHFGHRTRYWNPRMEEFIFGERNRIHIINLE
                     QTLPMLKDACSLAGKFAANGGRVLFVGTKRAARESIETEAQRCGMPYVSQRWLGGMLT
                     NFKTVKASIKRLLEMEKSVEDNSIARLTKKEQLMFNREIDKLRKSLGGIKTMTSLPDA
                     LFIIDTGYEKNAVSEARKLGIPVIAVVDTNNDPAGIDCVIPGNDDATRAIKVYTQTLA
                     DAIIEGRGSNANLRPAETFEEVEAREEAAQPRKPRAPRKPDSRGDKPRGRGKPRAAAE
                     APKAE"
BASE COUNT          886 a         1703 c         1785 g          935 t
ORIGIN      
        1 tccgtcctcg ataatcaccg ggctggccgt gggcggctcg agcacgccgc caatgcccac
       61 gccgcccgaa agatgaacgt gctttccgat ctgcgcgcac gagcccaccg tggcccaggt
      121 gtcgaccatg gtgttcttgc ccacgtaagc accgatgttt acgtacgagg gcatcaggat
      181 ggcgccgggc gaaatgaagg cgcccttgcg caccgccgcc ggcggcacca cgcgcgcgcc
      241 ctgcttggca aagtcgacgg cagtccagcc ggcgtatttc aaaggtacct tgtcgaagcc
      301 ggacagcgcg cccatctgga tcggtgcgtt gtcgtgcagg cggaagtaca gcagcaccgc
      361 cttcttcagc cactggttga cctgccagcc ggacgctgcc ggctcggcca cgcgcgcttc
      421 gcccttgtcg agcaggtcga tcgcctgggt gaccgcgtcg cggatggcgg gattgttctt
      481 gtcgagcgta tcgcgcgcgt tccacgcgga ctcgatggtg tcctggagct tgttcatctg
      541 catattgtcc caaaatttct gccaatccat tttcccctcc ccctttgcgg gaggggatca
      601 aggggatggg tgttgagccc tggcgaactg cttccgcgcg tcacggaaaa gaatgacccc
      661 tgccttcatc cctcccgcag ggggaaggaa gaacaagggc gcatcgcctt tggcgatgcg
      721 tacattgcct gtctttgccg ctacgcggct aagcggcttc gctgcggttg agcgtgcggg
      781 tgatgacctc gcgcagatca tccagcgcat cctcgctgac gatcggccgg tgcatgcggt
      841 cggtgatgta gaacacgtcc tcggcgcgct cgccgatggt gccgatcttg gccgcgtcga
      901 ggagaattcc acgcttctgg aatacgcggc cgatcagcga cagcaggccg ggctggtcgg
      961 cggtgaccag ttcgaggatg gtgcggttgc gcgccgcatc ctggccgaag tacaccatgg
     1021 tcggcgtgga gaaatgcctg agccgctgcg acgagcgccg gctgacgtcg atggtggaga
     1081 cgtctggatc ggacaacacc ttgcgcaact gcgtgcggat ttcctcgaag cgcagcgggt
     1141 tggcgatggc cgagccgtcg ctctcggtca ccgcgtagga atcgagcacg tagccgtcgc
     1201 gcgtggtgtg caggcgcgcg tcgaggatgt tcaggcccag gcgcgccagc acgccggtcg
     1261 acaggccgaa caggtgatcg cggtcgcgcg tatagacgaa caccgtggtg ccgcggccga
     1321 aggcggattc caccagcacc agcggcagtt cgccctccgg cacgcgcgcg atttccggcg
     1381 catgccaggc gagctcctcg ggcgaatagc gcaggaagta atcgtggtcg aagcgcgacc
     1441 atacgcgctc gaccgcggcg gcctcgatgt tgcgcgcggc cagcagtgcc agcgcgcggg
     1501 cgcgcttctc gctcaccatc tcgccttcga gcaccgggtt gttgaggccg cgctcgagcg
     1561 cgcgcgcggt ggaatcgtag agctgcttga gcaggctctc gcgccaggaa ttccacagcg
     1621 cggggttggt ggcgcggatg tcggcgcagg tcagcaggta gatgtagtcc aggcgcgcgc
     1681 gatcgcccac cttgagggcg aaatcggcca ccacctgcgg atcgctcacg tccttcttct
     1741 gcgcggtcag cgacatgacc aggtggttct tcaccagcca ggccaccaat tcggcatcgg
     1801 gatgcgacag gccgtgtttg aggcagaatt cctcggcctc gtgcgcgccg agctctgaat
     1861 ggtcgccgcc ccggcccttg gccatgtcgt ggaaaaatgc ggccaggtac agcagctcgg
     1921 gcttggccag gcggtccatc acctcgctgc aatgcggcag ctcgtcgcgg aagcgggcca
     1981 tggcgaagcg ccgcgcgttg cgcaccacgt acagcgtgtg ctcgtcgacg gtgagcgtat
     2041 ggaacaggtc gtactgcatc aggccgacga tcttgccgaa ggccggcagg tagcggccga
     2101 gcacgccgta gcggttcatg cggcgcaggt tgcgggtgag gccgcggccc tggcggaaca
     2161 tctcgatgaa cagcgcgcgg ctgcgcacat cctcgcgtac cgcctcggtc aggtagcggc
     2221 tgtcgcgcag gatcaggcgc agcgtctgcg cgcggatgcc gccgatcttc gggtgctgct
     2281 gcagcagcag gaagatttcc agcagcgcct gcggctggcg ctggaacacg tcgtcgctgc
     2341 gcgcctcgat gtagccgccg cagacggcaa agcgtgcgtt gagcggctcc gcggtgctgg
     2401 cctcgtcggg gcgcaggatc gcttcctcga acagctgcag cagcatgtcg ttcaggcagg
     2461 acagcgactt gatggtgcgg tagtacagct gcatgaactg ctcgaccgcg cggttcttgt
     2521 cgttgtcctc gtacccgaat acggcggcga tcttgatctg gtggtcgaac agcagccggt
     2581 cctcgcgccg gccggtgatc atgtgcaggg cgaagcgcac gcgccagagg aaatcctggc
     2641 cggcgaacag ctcgtcgcat tcctgcttgg tgaggaagcc gcggtcgcgc agctcgccca
     2701 gcgtctgtgc gccgaagtgc cgcttggcca cccagccgat ggtgtggatg tcgcgcagcc
     2761 cgccggggct ttccttgacg ttgggctcga gcttgtagcc ggtatcgtcg tacttgcggt
     2821 agcgaatcct ctgctcgttg cgcttggcct cgaagaattc cttgaccggc cacacgtggt
     2881 ccggcgtgat cgaggcctgc atctgcgcgt acaggcgcgt atcgccggtc agcaggcgcg
     2941 cttccatcat gttggtcatg acggtgatgt ccttggccgc ttcctgcgcg cactcgtccg
     3001 gggtgcgcac gctctggccc acttccaggc cgatgtccca gccgaaggcg gtcagctgct
     3061 ccagccgcgg gcggttgcgc tccagcgcgt cgccgtcgtg cagcagcagg atgtcgatgt
     3121 cggaatgcgg cagcagctcg ccgcggccgt aaccgcccac cgccaccatg gtcaggccgg
     3181 gcgggttgtc gggcaggaac ttctgccagg cggcgcgcag gatctcgtcg accaggtgcg
     3241 cgcgcgcgtg taccagcgaa tcggccggcg tgccgtcgtg gaacaggccg tagagccgct
     3301 cgcggcccca cttgagcgtg tcgcggaaag cggcgatggc gtaaccgccc tgggccaggc
     3361 gcgagcgcac tttttgcgcg ctgaatattc ccagctcctc gtcttcggcg aggtcgaact
     3421 cggcgcccat gagcgtgttt acatttcccc gtcgcgcagc gtcagcacgt cgtatccggt
     3481 ttcggtcacc agcacagtgt gctcccactg cgcagaaagg gaatggtctt tcgtcaccac
     3541 ggtccagccg tcgggcagca ggcgcacgtc ggggcggccg gcgttgatca tcggctcgac
     3601 ggtgatggtc atgcccgctt tcagttcaag tcccgtgcca ggcttgccgt agtgcagcac
     3661 ctgcggctcc tcgtggaaga tgcggccgat gccgtggccg cagtactcgc gcaccaccga
     3721 gtagccgcgc gcctcggtca ggctctggat ggcgtggccg atgtcgccca gccgcgcgcc
     3781 ggcttttacc tggcggatgc cggcgatcat cgcctcgtgc gcggtctgca ccaggcgccg
     3841 cgccagcacg gtcggctcgc cgacgaagaa catcttgctg gtgtcgccgt ggaagccgtc
     3901 cttgatcacg gtgatgtcga tgttgatgac gtcaccgcgc ttgagcacct tgtcgccggg
     3961 gatgccatgg cacaccacgt ggttgaccga ggtgcagatg ctcttgggaa agcccttgta
     4021 gttgagcggc gccggcgtgc agcgcagttc atcgacaatg tagttgtggc agatctggtt
     4081 gagcagctcg gtggtcacgc ccggcttcac gtgcgggccg atcatctgca gcacctgcgc
     4141 cgcggcggcg ccggcggcac gcatctgctg ctgctcttcg gggttcttga gggtgatgga
     4201 cacggacgag tcttgggggc ggaaagtcga gattcgagtg ccgattttcg aatccacgga
     4261 aatcgaagcc cggctcgttg tttgcgaagg ggcgctatgg tataaagcgc gcccttcaaa
     4321 ggcaatttcg ccttgaagaa atccattcac gtccatcctg ggtgatcttc accggacgtg
     4381 atttgccatc aacccaagga gtttttcatg gcgaccatca ccatgcgcca gctgctggaa
     4441 gcgggcgtcc acttcggaca ccgcacccgc tactggaacc cgagaatgga agaattcatt
     4501 ttcggcgaac gcaaccgcat ccacatcatc aacctcgagc agacgctgcc gatgctcaag
     4561 gacgcgtgct cgctggcggg caagttcgcg gccaacggcg gccgcgtgct gttcgtcggc
     4621 accaagcgcg ccgcgcgcga gtcgatcgag accgaggcgc agcgctgcgg catgccctac
     4681 gtgtcccagc gctggctggg cggcatgctg accaacttca agacggtgaa ggcgtcgatc
     4741 aagcgcctgc tggaaatgga aaagagcgtg gaggacaact ccatcgcgcg cctgaccaag
     4801 aaagagcagc tgatgttcaa ccgcgagatc gacaagctgc gcaagtcgct cggcggcatc
     4861 aagaccatga ccagcctgcc ggacgcgctg ttcatcatcg acaccggcta cgagaagaac
     4921 gccgtatcgg aagccaggaa gctgggcatc ccggtgatcg cggtggtcga caccaacaat
     4981 gatcccgcgg gcatcgactg cgtgatcccg ggcaacgacg acgccacccg cgcgatcaag
     5041 gtctacaccc agaccctggc cgacgccatc atcgagggcc gcggctccaa cgcgaacctg
     5101 cgcccggccg agacgttcga agaagtggaa gcgcgcgagg aagcggcgca gccgcgcaag
     5161 ccgcgcgcgc cgcgcaagcc cgactcgcgt ggcgacaagc cgcgcggccg cggcaagccg
     5221 cgtgccgccg ccgaggcgcc caaggccgag taaggctggc gcgagccgca acggtggccc
     5281 ggccagcaat gcccgggcca tcttcattt
//
LOCUS       DASZZC010000133         6773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_392994,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000133 DASZZC010000000
VERSION     DASZZC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392994"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2470)
                     /gene="acnA"
                     /locus_tag="VHE37_03090"
     CDS             complement(<1..2470)
                     /gene="acnA"
                     /locus_tag="VHE37_03090"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HVT34543.1"
                     /translation="MKDSFKSKSTLKVGSKSYSYFSLKALEPKFNLARLPYSYKILLE
                     NLLRHEDGENTTAADVEALAGADLKKVPAKDINFTPARVVLQDFTGVPCVVDLAAMRD
                     AITQLGGKASQVNPLCPVELVIDHSVMIDHYGSKDALDLNAKVEFQRNQERYTFLRWG
                     QEALKNFKAVPPDTGIVHQVNIEYLARVVFENNGTVYPDSCFGTDSHTTMVNGIGVLG
                     WGVGGIEAEAAMLGQPSSMLIPEVIGVRFTGKLAEGATATDLVLTVTEMLRKKGVVEK
                     FVEYFGPGLANLSASDRNTIANMGPEYGATCGIFPIDDETLNYLRLTGRSDEQIELVK
                     AYAQAQGMWWTPHGPEPEYTDVLHLDLGSIKPSLAGPKRPQDRVLLSDVKSNFRKAFE
                     AEQKLRPSKGPATVTDNGKTFELKDGAVMIAAITSCTNTSNPSVLIGAGLLARKARAL
                     GLKSQPWVKTSLAPGSLAVTEYLKKANLIEDLESLGFFVTAYGCTTCIGNSGPLNEPL
                     SKAINDNTLSVSAVLSGNRNFEGRVHQDVRMNYLASPPLVVAFAIAGTTDIDLTSEPI
                     GKDKNGKDVFLKDIWPSNKEISDAVTKAVTSDLYKASYADVLKGDARWQSIKVNKSET
                     YNWDAKSTYVQNPPYFVGMKKTPPGIPTITGARALAVLGDSITTDHISPAGDIKKDGP
                     AGKYLMEHGVQRADFNSFGSRRGNHEIMMRGTFANTRIKNAMTPGVEGGVSRHINGSA
                     GPVEPIYDVAMKYQKDGVPLVVLGGKEYGTGSSRDWAAKGTILLGVKAVITESFERIH
                     RANLVGMGVLPLNFTNGQNAASLGL"
     gene            2649..5849
                     /locus_tag="VHE37_03095"
     CDS             2649..5849
                     /locus_tag="VHE37_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34544.1"
                     /translation="MIAGMLLLALAAHTATAGVVPTAGLTQTQFLHTLLIASSLLLVC
                     GVALLIGVRRMRHEIEERRYSEARIEGAQKLLREVTDRIPNGVVFQFSRLKDGSLKTN
                     FVSDGILQVAGIDRREVLKNYQLVFDSIHEDDRARVRAAIEESAATRQPYQIEYRVRS
                     PRGTIEWMRGSAVPRPGPDGSMVWNGFTTQISDLKKIESEIRSAQAVLHEVTDAIPGG
                     VYRFRMKPDGHLELLFSNSGIKRLMGLKSEDEVGGFDNILHYVIDEDRRVILVDIKES
                     ARTLQPVQRDVRIRRGDDEPLWIHAAAVPQRDEDGGVTWNGYAVDITEHKTLEADLAS
                     AREYVIEIAQGLPGVVFQAQVKFDGQVQLLFGGEGYFKLMGVEHAGTIIEWQKLLSAV
                     YADDRELVMNAITHSASDEGKPIQLDFRVGSESAQRWVRIEAIPKTPGRREVMALWNG
                     YGIDITERKQLERELAQAKEAAEAANRAKGEFLANMSHEIRTPMNAIIGLAHLALRTN
                     PDERQRDYLQKIQSSAQSLLGVINDILDFSKIEAGKLGLEQTTLRLEDVLASLANVIS
                     VKAAEKGLELLFSVPLDVPAQLIGDPLRLGQVLLNLAGNAVKFTEHGQIVVSAKVVER
                     RSEHVVLEFSVADSGIGMTGEQQARLFESFSQADTSTTRKYGGTGLGLSISKRLVEIM
                     GGSISVESETGRGSTFRFTVPLGLPAEELDHAHAARPQVAGTAILIVDDNASSREIFD
                     SYLRSFGFAPGVVHSAEQAVAELQQADAEGRPYRLVLLDWQMPEMDGIEAARAIRALP
                     LAQMPAIVMVSAYGREELTRQVEALGLDGLLLKPVNASVLLNTVMQTLGSDAGVRVAG
                     GERAAEPSSALAGARVLVAEDNPLNQQVVRELLDGAGVSVSVVGDGRSCVDHALADSC
                     DAVLMDLQMPGMDGLEATRRIREQRPALPIIAMTASAMANDRERCLAAGMNDYLSKPI
                     DVDQLFQVLARWLPRRGVPERERIIALMASLRAQLSANDSGAIDTLDAIGGLLDAGVR
                     QRALRDISRMIDGFNFSAALSRLPEVARQLGLEQGA"
     gene            5846..>6773
                     /locus_tag="VHE37_03100"
     CDS             5846..>6773
                     /locus_tag="VHE37_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVT34545.1"
                     /translation="MNASLPTVLVVDDTPQNLNVASRILREQYRALVALDGTRALEIA
                     RASPPPDLILLDVMMPGMNGYEVCRQLKADARTRDIPVIFLTALDEANSEAAGFEAGA
                     VDYITKPISPPTMLARVRTHLRLRAHERHLADLVHERTRALEQRTRELEQTRLEVIRR
                     LGRAAEYKDDATGLHVIRMSHYTRLLALAAGMSSDRAELLFHAAPMHDIGKIGIPDHI
                     LQKPGALTDEEWVVMKQHPAIGAGIIGLQHGELLETARLVALTHHEKWDGSGYPRARA
                     GKDIPVEGRIVAVADVFDALTSKRPYKNAWSVE"
BASE COUNT         1110 a         2184 c         2325 g         1154 t
ORIGIN      
        1 cgagcccgag cgaagcggcg ttctggccgt tggtgaagtt cagcggcagc acacccatgc
       61 cgaccaggtt ggcgcggtgg atgcgctcga agctctcggt gatcaccgcc ttcacaccaa
      121 gcaggatggt gcccttggcc gcccagtcgc gcgacgagcc ggtgccgtac tccttgccgc
      181 cgagcacgac cagcggcacg ccgtccttct ggtatttcat ggccacgtcg tagatcggct
      241 cgaccgggcc agcggaacca ttgatgtgac gcgacacgcc gccttccacg ccgggcgtca
      301 tcgcgttctt gatgcgcgtg ttggcgaagg tgccgcgcat catgatctcg tgattgccgc
      361 ggcgcgagcc gaagctgttg aagtcggcgc gctgcacgcc gtgctccatc aggtatttgc
      421 cggcagggcc gtccttcttg atgtcgccgg cgggcgagat gtggtcggtg gtgatggaat
      481 cgcccagcac cgccagcgcc cgcgcgcccg tgatggtggg tatgcccggc ggcgtcttct
      541 tcatgccgac aaagtacggc gggttctgca cgtaggtgct cttcgcatcc cagttgtagg
      601 tctcgctctt gttcaccttg atcgactgcc agcgcgcgtc acccttgagc acgtcggcgt
      661 agctcgcctt gtacaggtcg gacgtcaccg ccttggtcac ggcgtcgctg atctccttgt
      721 tgctcggcca gatgtccttg aggaagacat ccttgccgtt cttgtccttg ccgatcggct
      781 cgctggtgag gtcgatgtcg gtggtgccgg cgatggcgaa ggccaccacc agcggcggcg
      841 aggccaggta gttcatgcgc acgtcctggt gcacgcggcc ctcgaagttg cggttgcccg
      901 acagcaccgc cgaaaccgac agcgtgttgt cgttgatcgc cttggacagc ggctcgttga
      961 gcgggccgga gttgccgatg caggtggtgc agccgtaggc ggtgacgaag aagcccagcg
     1021 attcgaggtc ttcgatcagg ttggctttct tcaggtactc ggtcaccgcc agcgagccgg
     1081 gggcgagcga ggtcttgacc cagggttgcg acttcagccc cagcgcacgc gccttgcgcg
     1141 ccaagaggcc cgcgccgatc agcacgctcg ggttggaggt gttggtgcac gaggtgatcg
     1201 ccgcgatcat caccgcgccg tccttcagtt cgaaggtctt gccgttgtcg gtgacggtgg
     1261 ccgggccctt cgacgggcgc agcttctgct cggcctcgaa cgccttcctg aagttgctct
     1321 tcacgtcgga cagcagcacg cggtcctgcg ggcgcttggg gccggccagg ctgggcttga
     1381 tgctgcccag gtccaggtgc agcacgtcgg tgtactccgg ctccggtcca tgcggcgtcc
     1441 accacatgcc ctgcgcctgc gcgtaggcct tgaccagctc gatctgctcg tcgctgcggc
     1501 cggtgaggcg caggtagttc agcgtctcgt cgtcgatcgg gaagatgccg caggtggcgc
     1561 cgtattccgg gcccatgttg gcgatggtgt tgcggtcgct ggcggagaga ttggccaggc
     1621 ccgggccgaa gtattcgacg aacttctcga ccacgccctt cttgcgcagc atctcggtga
     1681 cggtgagcac caggtcagtc gcggtggcgc cttcggccag cttgccggtg aaacgcacgc
     1741 cgatcacttc cgggatcagc atcgacgagg gctggccgag catggcggct tccgcctcga
     1801 tgccgcccac gccccagccc agcacgccga tgccgttcac catggtggta tggctgtcgg
     1861 tgccgaagca ggaatccggg tacaccgtgc cgttgttctc gaacaccacg cgcgccaggt
     1921 actcgatgtt cacctggtgc acgatgccgg tgtcgggcgg caccgccttg aagttcttca
     1981 gcgcttcctg gccccagcgc aggaaggtgt agcgctcctg gttgcgctgg aattcaacct
     2041 tggcattgag gtccagggcg tccttgctgc cgtagtggtc gatcatcacc gagtggtcga
     2101 tgaccagctc gaccgggcac agcgggttca cctggctggc cttgccgccc agctgggtga
     2161 tggcgtcgcg catggcggcc aggtcgacga cgcagggcac gccggtgaag tcctgcagca
     2221 ccacgcgcgc gggggtgaag ttgatgtcct tggccgggac ctttttcagg tcggcgccgg
     2281 ccagcgcctc cacgtcggcg gcagtggtgt tctcgccgtc ttcatggcgc aacaggttct
     2341 cgagcaggat cttgtacgag tagggcaggc gcgcaagatt gaacttgggc tccagcgcct
     2401 tgaggctgaa gtaggaatag ctcttcgagc cgactttgag ggtggatttg ctcttgaagc
     2461 tgtccttcat ggcagtgcgc tccggatgcg ggcggtaggg agatttttgg gggcgcgaag
     2521 tataccaaag cgcctgccgg ctccccgttg ctgagcctgg gcagttgcac tactgtccat
     2581 ctgtccggaa cgcaaataat tcgaatgcgc gggggaggga gcagcgcatg cttcgacggg
     2641 ctggcgggat gatcgcgggg atgctgctgc tggcgctggc tgcgcacacg gcgacggcgg
     2701 gcgtcgtgcc gaccgcgggc ctgacgcaga cgcaattcct gcacacgctg ctgattgcca
     2761 gcagcctgct gctggtttgc ggcgtggcgc tgctgatcgg cgtgcgccgc atgcgccacg
     2821 agatcgagga gcggcgctac tccgaggcgc gcatcgaggg tgcgcagaag ctgctgcgcg
     2881 aagtcaccga ccgcattccc aacggcgtgg tgttccagtt cagccgcctg aaggacggct
     2941 cgctcaagac caacttcgtg tccgacggca tcctgcaggt tgccggcatc gaccgtcgcg
     3001 aggtgctgaa gaactaccag ctggtgttcg acagcatcca cgaggacgac cgcgcgcgcg
     3061 tgcgtgccgc catcgaggaa tccgccgcca cgcgccagcc gtaccagatc gagtaccgcg
     3121 tgcgttcgcc gcgcggcacc atcgagtgga tgcgcggctc cgcggtgccg cggccggggc
     3181 cggacggcag tatggtgtgg aacggcttca ccacgcagat cagcgacctg aagaagatcg
     3241 agtcggagat ccgctcggcg caggccgtcc tgcacgaggt caccgatgcc atcccgggcg
     3301 gcgtctaccg tttccgcatg aagccggacg gccatctcga gctgctgttc tccaactccg
     3361 gcatcaagcg cctgatgggg ctgaaatccg aagacgaggt cggcggcttc gacaacatcc
     3421 tgcattacgt gatcgacgag gaccggcgcg tgatcctggt cgacatcaag gaatcggcgc
     3481 gcaccctgca gccggtgcag cgcgacgtgc gcatccgccg cggcgacgac gagccgttgt
     3541 ggatccacgc cgccgcggtg ccgcagcgcg acgaggacgg cggcgtcacc tggaacggct
     3601 acgccgtcga tatcaccgag cacaagaccc tggaagccga cctggcatcg gcgcgcgagt
     3661 acgtgatcga gattgcgcag ggcctgcccg gcgtggtgtt ccaggcacag gtgaagttcg
     3721 acggccaggt gcagctgctg ttcggcggcg agggctactt caagctgatg ggcgtggagc
     3781 acgccggcac catcatcgaa tggcagaagc tgctgtcggc ggtctacgcg gatgaccgcg
     3841 aactggtgat gaatgccatc acccattcgg ccagcgacga gggcaagccg atccagctcg
     3901 atttccgcgt cggcagcgag agcgcgcagc gctgggtgcg catcgaagcg attcccaaga
     3961 cgcccgggcg gcgcgaggtg atggcgctgt ggaacggcta cggcatcgac atcaccgagc
     4021 gcaagcagct ggagcgcgag ctggcgcagg ccaaggaggc ggccgaggcg gccaaccgcg
     4081 ccaagggcga gttcctggcc aacatgagcc acgagatccg cacgccgatg aacgccatca
     4141 tcggtctggc gcacctggcg ctgcgcacca acccggacga acgccagcgc gattacctgc
     4201 agaagatcca gtcatcggcg cagtcgctgc tgggcgtgat caacgacatt ctcgattttt
     4261 ccaagatcga ggccggcaag ctgggactgg agcagaccac gctgcgcctg gaagacgtgc
     4321 tggccagcct ggccaacgtg atcagcgtga aggccgcgga gaaaggcctg gagctgctgt
     4381 tctcggtgcc gctcgatgtg cccgcgcaac tgatcggcga tccgctgcgc ctgggccagg
     4441 tgctgctcaa cctcgccggc aacgcggtga aattcaccga acatggacag atcgtggtgt
     4501 cggccaaggt ggtcgagcgg cgcagcgagc acgtggtgct ggagttttcc gtggccgatt
     4561 ccggcatcgg catgaccggt gagcagcagg cgcgcctgtt cgaatcgttc tcgcaggccg
     4621 acacgtccac cacgcgcaaa tacggcggca ccgggctggg cctgtccatc agcaagcggc
     4681 tggtggagat catgggcggc agcatcagcg tggagagcga gaccggccgc ggcagcacct
     4741 tccgcttcac cgtgccgctg ggcctgccgg ccgaggaact ggaccatgca catgcggcca
     4801 gaccgcaggt ggcgggcacc gccatcctga tcgtcgacga caacgcttcc tcgcgcgaga
     4861 tcttcgattc ctacctgcgc tccttcggtt ttgcgccggg cgtggtgcac tcggccgagc
     4921 aggccgtggc cgaactgcag caggctgacg ccgagggcag gccctatcga ctggtgctgc
     4981 tcgactggca gatgccggaa atggacggca tcgaggcggc gcgcgccatc cgcgcattgc
     5041 cgctggcgca gatgccggcc atcgtcatgg tgtctgccta tgggcgtgag gaactcaccc
     5101 ggcaggtcga agcactgggg ctggacggcc tgctgctcaa gccggtcaat gcctcggtgc
     5161 tgctcaacac cgtgatgcag acgctgggca gcgatgccgg cgtgcgcgtg gccggcggcg
     5221 aacgcgcggc cgagccatcc agcgcgctgg cgggcgcgcg cgtgctggtg gccgaggaca
     5281 atccgctgaa ccagcaggtg gtgcgcgagc tgctcgacgg cgccggcgtc agcgtcagcg
     5341 tggtcggtga cggccgcagc tgtgtcgatc acgcgctggc cgactcctgc gatgccgtgt
     5401 tgatggatct gcagatgccg ggaatggatg ggctcgaagc cacccgccgc atccgcgagc
     5461 agcggcccgc gctgccgatc atcgccatga cggccagcgc catggccaat gaccgcgagc
     5521 gctgcctggc cgccggcatg aacgattacc tgtccaagcc catcgatgtg gatcagctgt
     5581 tccaggtgct ggcgcgctgg ttgccgcgcc gcggcgtgcc ggagcgcgag cgcatcatcg
     5641 cgctcatggc cagcctgcgt gcgcagctca gcgccaacga ttccggcgcc atcgacacgc
     5701 tcgatgccat cggtggcctg ctcgacgccg gcgtacgcca gcgcgcgctg cgcgacatct
     5761 cgcgcatgat cgacggcttc aacttcagcg ccgccctcag ccgtctgccc gaagtggcgc
     5821 gccagctggg cctggagcag ggcgcatgaa cgcatcgttg cctacggtcc tggtggtcga
     5881 cgacacgccg cagaacctca acgtggccag ccgcatcctg cgcgagcaat accgcgcgct
     5941 ggtggcgctg gacggcacgc gcgccttgga gattgcgcgg gcctcgccgc cgccggacct
     6001 gatcctgctc gacgtgatga tgcccggcat gaacggctac gaggtttgcc ggcagctgaa
     6061 ggccgatgcg cgcacgcgcg acatcccggt catcttcctc actgcgctgg acgaggccaa
     6121 cagcgaagca gccggattcg aagctggggc ggtcgactac atcaccaagc cgatcagtcc
     6181 gccgaccatg ctggcccgcg tgcgcacgca cctgcgcctg cgcgcgcacg agcgccacct
     6241 ggcagacctg gtgcacgagc gcaccagagc gctggagcag cgcacgcgcg agctggagca
     6301 gacccgcctg gaagtgatcc ggcgcctcgg ccgcgccgcg gagtacaagg acgacgccac
     6361 cggcctgcac gtcatccgca tgagccatta cacgcggctg ctcgcactcg ccgccggcat
     6421 gtccagcgac cgcgccgagc tgctgttcca tgctgcgccg atgcacgata ttggaaaaat
     6481 cggcatcccc gatcacatcc tgcagaaacc cggcgcgctg actgatgagg aatgggtggt
     6541 gatgaagcag cacccggcca tcggcgcggg catcatcggc ctgcagcatg gtgagctgct
     6601 ggaaacggca cggctggtgg cgctgaccca ccacgagaaa tgggatggaa gcggctaccc
     6661 gcgcgcgcgt gcaggcaagg acattccagt tgaggggcgc atcgtcgcag tggccgacgt
     6721 gttcgatgcg ttgacgtcaa agcgcccgta caagaacgca tggtccgtcg agg
//
LOCUS       DASZZC010000134         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_139712,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000134 DASZZC010000000
VERSION     DASZZC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139712"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..433)
                     /locus_tag="VHE37_03105"
     CDS             complement(29..433)
                     /locus_tag="VHE37_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT34546.1"
                     /translation="MRFRRHEQPAEDTGIDLAPMLDFFLNLLIFFIITAAFVTESGIK
                     VNRPSAKTAVKQEKSNILIAISKGSEIWIDRQHVDIRALRPLIQKMKLENPNAAVIVQ
                     ADRDAKAALLVDVMDQARLAGVKDVAIAATPK"
     gene            complement(430..981)
                     /locus_tag="VHE37_03110"
     CDS             complement(430..981)
                     /locus_tag="VHE37_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT34547.1"
                     /translation="MLAEISGALPAALAAPFESLDDLFDMGGPVLTWIFLAALVMWTL
                     IVERYWFFAFVFPQRRAELRRAWEARDNRRSWTSRRIRQAMIGEMATAMASTLPVMRV
                     IIPICPLLGLLGTVTGMLEVFDIMALKGSVDARAMAYGVSHAMISTLTGLGVAVSGMF
                     FVNRFNARVRVETELLSDALVYQ"
     gene            complement(971..>2048)
                     /locus_tag="VHE37_03115"
     CDS             complement(971..>2048)
                     /locus_tag="VHE37_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT34548.1"
                     /translation="TTRAGDYQQVYATVKSAAADFRAVAGGSLVTAQAPDRLAFLDTL
                     SQGREIPGIAELEKFWFVLQQEMTESGKVARFNADFLDENGVARKGDLVRIGTFTAFD
                     ADGAYLVLDGARLAALPHQPSGYRGMAKDFASEKSGFADAMIDPSRGDLLKLEAARPD
                     MMERVQQGGIVGYVIITVAIAGFLMAVFQMVYLSIVERRVRGQLRNTSAPGSDNPLGR
                     VLLALRDARAPDPNSEMTDAEVLELRLSEAVLRETPKLERFQSIIRLIVAAGPLLGLL
                     GTVSGMIITFQVITELGAGDPKAMARGISQAMVSTVLGLGEAIPLLFINAYLASRSKV
                     LIQILDEQSAGLLARSLEARKNAG"
BASE COUNT          357 a          680 c          685 g          326 t
ORIGIN      
        1 gccgcgctcc tacaaagcaa tagcaaactc atttgggtgt cgcggcgatg gccacgtcct
       61 tcacgcccgc cagccgcgcc tggtccatca catcgaccag cagcgcggcc ttggcgtcgc
      121 ggtcggcctg cacgatgacc gcggcgttgg ggttctccag cttcatcttc tggatcagcg
      181 gacgcagtgc acggatgtcc acgtgctggc ggtcgatcca gatttcgctg cccttggaga
      241 tggcgatgag gatgttgctc ttctcctgct tcaccgcggt cttggcgctg ggccggttga
      301 ccttgatgcc gctctcggtg acgaaggcgg cggtgatgat gaagaagatg agcaggttga
      361 ggaagaaatc cagcatcggc gccaggtcga tgccggtgtc ctcggcgggc tgttcgtggc
      421 ggcggaatct cattggtaca ccagcgcgtc ggacagcagt tcggtctcca cgcgcacgcg
      481 cgcgttgaag cggttgacga agaacatgcc gctgaccgcg acacccaggc cggtgagcgt
      541 cgagatcatg gcgtgcgaaa ccccataggc catggcacgc gcgtccaccg agcccttcag
      601 cgccatgatg tcgaacacct cgagcatgcc ggtgacggtg ccgagcaggc cgagcagcgg
      661 gcagatggga atgatcacgc gcatcaccgg cagcgtggag gccatggcgg tggccatctc
      721 gccgatcatc gcctggcgga tgcggcgcga ggtccagctg cgccggttgt cgcgcgcttc
      781 ccaggcacgc cgcagttccg cgcggcgctg cgggaagacg aaggcgaaga accagtagcg
      841 ctcgacgatc agcgtccaca tgaccagcgc ggcgaggaaa atccaggtga gcaccgggcc
      901 gcccatgtcg aacaggtcgt cgagcgactc gaacggcgcc gccagcgccg ccggcaatgc
      961 accggaaatc tcagccagca tttttcctgg cctccagcga acgcgcgagc aggcccgcgg
     1021 attgctcgtc gaggatctgg atcagcacct tcgagcgcga cgccaggtac gcgttgatga
     1081 acagcagcgg gatcgcctcg cccaggccga gcacggtgga caccatggcc tggctgatgc
     1141 cgcgcgccat ggccttggga tcgcctgcgc ccagctcggt gatgacctgg aaggtgatga
     1201 tcatgccgct caccgtgccg agcaggccga gcagcgggcc ggcggcaacg atcaggcgga
     1261 tgatcgactg gaagcgctcc agcttcggcg tctcgcgcag caccgcttcc gacaggcgca
     1321 gttccagcac ctcggcatcg gtcatctcgc tgttgggatc gggcgcgcgc gcatcgcgca
     1381 gcgccagcag cacgcggccc agcggattgt cgctgcccgg cgcagaagtg ttgcgcagct
     1441 gcccgcgcac gcggcgctcg acgatggaca ggtacaccat ctgaaacacc gccatgagga
     1501 agccggcgat ggccacggtg atgatgacgt agccgacgat gccgccctgc tgcacgcgtt
     1561 ccatcatgtc gggccgcgct gcttccagct tgagcaggtc gccgcgcgag ggatcgatca
     1621 tggcgtcggc gaaaccgctt ttctccgagg caaaatcctt cgccatgccg cggtagccgg
     1681 aaggctggtg cggcagcgct gccagccgcg cgccgtcaag cacgagataa gcaccatcgg
     1741 catcgaacgc ggtgaaggtg ccgatgcgca ccaggtcgcc cttgcgcgcc acgccgttct
     1801 cgtcgaggaa gtccgcgttg aagcgcgcca ccttgccgct ctcggtcatc tcctgctgca
     1861 gcacgaacca gaatttctcc agctcggcga tgccaggaat ctcgcggccc tgcgacagcg
     1921 tgtcgaggaa cgccagccgg tccggcgcct gcgcggtgac cagcgagccg ccggccaccg
     1981 cgcggaaatc cgccgccgcc gacttcaccg tggcgtaaac ctgctggtag tcgccggcgc
     2041 gcgtggtc
//
LOCUS       DASZZC010000135         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1104406,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000135 DASZZC010000000
VERSION     DASZZC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104406"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..638)
                     /locus_tag="VHE37_03120"
     CDS             complement(90..638)
                     /locus_tag="VHE37_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34549.1"
                     /translation="MNKLILSGLVATLAAAPAFADEIDYARVVSATPIYHDVRIDTPR
                     QECTTERVVYNQGYQPNPGAVMLGAVLGGVVGHQFGGGMGNAVATGAGALIGANVGAR
                     SGGYYGGERVVDRPVCRTYSEYRVEQRLDGYDVAYRYNGRIYHTHTPYDPGNQIAVHV
                     DVAPVQYGPQYGPQYDNYPRYD"
     gene            complement(754..2031)
                     /locus_tag="VHE37_03125"
     CDS             complement(754..2031)
                     /locus_tag="VHE37_03125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT34550.1"
                     /translation="MEPDKAPARPARADPEAVRFWRFNQPAVAAGASPDARSELDQAT
                     RRQLEQIARARTGRFRFPGELEQRFQRFVRESNRVARLSISLLTLVLFVSAPWWSPLV
                     LGLPDASKPYTTMVCLAVVAPVFAVASLLQYHKAQSEWTEWFLMLAFLLEVAAIELMR
                     YATQRAGFFITPAITATVPVCFIPMAQLRFSRCVVFGAGYVGVMIASALLLPYQLGMR
                     DPSEWLTELILIVTAIAATAFSKLATRRAWASNLLLDTMAAHDALTGLPNRTAFEQHF
                     ELMTNTAQREKKPMLLALVDLDHFKLVNDSYGHAYGDGVLNEIGLLLGEFARRSSDIA
                     ARLGGEEFALLLYDCSLDGARTRLNELLNMVRELNIEHKQNAAGVITVSIGATSVVPG
                     AQLAEVYHVADDMLYRVKRAGRNHLCLADDVKG"
     gene            complement(2089..3423)
                     /locus_tag="VHE37_03130"
     CDS             complement(2089..3423)
                     /locus_tag="VHE37_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT34551.1"
                     /translation="MKKIPVILLLLALCAPALAKVTPEEAAKLGAELTPMGAEVAGNK
                     DGTIPKWEGGLPKKDMARGENPYAADKPLYTITAANMAQYDAVLTEGYKNLFKTFPDY
                     KMIVYPTRRSASYPQWFYDATKKNATTVELTDNGYGFCCTAQGYPFPIPKNGTEVMWN
                     HIMRYNTKGFRGYINAATTQADGSYVIERSYLELSYMYNNVKSTVDSLKGMNLYAFTK
                     TVSPPNEAGEAHLLHVPIDRIKNETGVWFYSPSQGRVRRIGEVGYDNPLYDGLMTHDQ
                     LDMFNGPLDRYTIKLLCKKELIVPYNSYKIYDPKLKYKDIVTKGHINQDLARYELHRV
                     WVIEAQVRQGFNHRYKRRTFYLDEDSWIALLQDMYDERDQFWRTAESHLICFANVPVV
                     VNGVQVHYDLQSRRYVAINMTNEERKMIDYDWEAKPDYFQPTSLKKFAESAQ"
     gene            complement(3440..>4603)
                     /locus_tag="VHE37_03135"
     CDS             complement(3440..>4603)
                     /locus_tag="VHE37_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT34552.1"
                     /translation="QELGDRLWRHVANLALPRSAVPALVIAAIAFGWSVWKYPDLRIG
                     DVQTGVPELKPDSRYNQDSAEIARNFFIGVDVLKVIAQVPQYGCVQKPLMETIDNYAW
                     HIQNTPGVASVLSLPQVARFLNEGLSEGAPKWLVLPRNRAALSETTHAVPSTAGLQNL
                     DCSAMPVQIYTTDHKAETISRIVAATKAYNQEHQDEGVNFALASGNVGVMAATNEEIK
                     RTELTVIIWVHLTLLIFVLISFRSFAAVICIITPLVLCSLLTYGGMATLDIGMKSATL
                     PVAAFGVGIGVDAGIYIWSVIARYLEEGYGLRDAYFEALRRTGKAALFTSLSLIVAVF
                     TWLFSDLQFQRDMGALLLFMFTTNLFGAILLLPALAWVVLPRSGGNAAAPAAV"
BASE COUNT          755 a         1446 c         1553 g          849 t
ORIGIN      
        1 aaggcgggct gcatcagcag cccgcctgcc agcaagatcc acatcagggt gcgcttgcgc
       61 atggaacgat tctagcgccc ggcgccgttt cagtcgtagc gcgggtagtt gtcgtactgc
      121 gggccatatt gcggcccgta ctgcaccggc gccacgtcga cgtgcacggc gatctggttg
      181 cccggatcgt acggcgtatg cgtgtggtag atgcggccgt tgtagcggta ggccacgtcg
      241 tagccgtcca ggcgctgctc tacgcggtac tcgctgtagg tgcggcacac cgggcggtcg
      301 accacgcgct cgcctccgta gtagccgccg ctgcgcgcgc ccacgttggc gccgatcagc
      361 gcgccggcgc cggtggccac cgcgttgccc atgccgccgc cgaactggtg gccgaccacg
      421 ccgcccagca ccgcgccgag catcaccgcg ccggggttgg gctggtagcc ctggttgtag
      481 accacccgct cggtggtgca ttcctgccgg ggagtgtcga tgcgcacgtc gtggtagatc
      541 ggcgtggcgc tgaccacgcg cgcatagtcg atttcatcgg cgaaagccgg cgccgccgcg
      601 agggtggcca ccagaccgct caggatcaac ttgttcatga cgctgctccc ggttgacgca
      661 gccgccggaa tggcggggtt acgggagaga ctctagggga tgaggctgaa ttgaccctga
      721 atgtgcggtg ggagaggcac ttgccgcggc aacttaacct ttcacgtcgt cagccaggca
      781 caggtggttg cggccggcgc gcttgacccg gtacagcatg tcgtcggcca cgtgatagac
      841 ctccgccagc tgcgcacccg gcaccacgga cgtggcgccg atcgacacgg tgatcacgcc
      901 ggcggcgttc tgtttatgct cgatgttgag ctcgcgcacc atgttgagca gctcgttgag
      961 gcgcgtgcgc gcgccgtcga gcgagcagtc gtacagcagc agggcgaatt cctcgccgcc
     1021 cagccgcgcg gcgatgtcgc tcgagcggcg cgcgaactcg cccagcagca ggccgatctc
     1081 gttgagcacg ccgtcgccgt aggcatggcc gtagctgtcg ttgaccagct tgaagtggtc
     1141 caggtcgacc agcgccagca gcatcggctt cttctcgcgc tgcgcggtgt tggtcatcag
     1201 ctcgaagtgc tgctcgaacg cggtgcggtt gggcaggccg gtgagcgcgt cgtgcgcggc
     1261 catggtgtcg agcagcaggt tggaggccca ggcacggcgc gtcgccagct tcgagaaggc
     1321 ggtggcggcg atggcggtga cgatcaggat cagctcggtg agccattcgc tcggatcgcg
     1381 catgcccagc tggtagggca gcagcagcgc gctggcgatc atcacgccga cgtagccagc
     1441 gccgaacacc acgcagcgcg agaagcgcag ctgcgccatc gggatgaagc acaccggcac
     1501 ggtggcggtg atcgccggcg tgatgaaaaa gccggcccgc tgcgtggcgt agcgcatcaa
     1561 ctcgatggcg gcaacttcca gcaggaaggc cagcatcaga aaccattcgg tccattcgct
     1621 ctgtgccttg tggtactgca gcagcgaggc caccgcgaac accggcgcca ccaccgccag
     1681 gcacaccatg gtcgtatacg gcttgctggc atcgggcagg cccagcacca gcggcgacca
     1741 ccacggtgcg gagacgaaca gcaccagcgt cagcaggctg atggacagcc gcgcgacgcg
     1801 gttggattcg cgcacgaagc gctggaagcg ctgttccagt tcgcccggga agcggaagcg
     1861 cccggtgcgc gcgcgggcaa tctgctcgag ctggcggcgg gtggcctggt ccagttcgga
     1921 gcgcgcatcc ggtgatgctc cggccgcgac ggccggctgg ttgaagcgcc agaaacgcac
     1981 ggcctccggg tcggctcgtg caggccgcgc tggcgccttg tccggttcca tgcccacgct
     2041 cactccctga acgtctgcgg gtggataatg gccaatatta tcgcgcgatt attgcgcgct
     2101 ttcggcgaat ttcttcagcg aggtcggctg gaagtagtcc ggcttggctt cccagtcgta
     2161 atcgatcatc ttgcgttctt cattggtcat gttgatcgcc acgtagcggc gcgactgcag
     2221 gtcgtaatgc acctgcacgc cattgaccac caccggcacg ttggcgaagc agatcaggtg
     2281 cgactccgcg gtgcgccaga actggtcgcg ttcgtcgtac atgtcctgca gcagcgcgat
     2341 ccacgaatct tcatcgagat agaacgtgcg gcgcttgtag cgatggttga agccctggcg
     2401 cacctgcgcc tcgatcaccc atacgcggtg caactcgtag cgcgccaggt cctggttgat
     2461 gtggcccttg gtgacgatgt ccttgtactt cagcttgggg tcgtagatct tgtagctgtt
     2521 gtacgggacg atcagttcct tcttgcacag cagcttgatc gtgtaccggt ccagcgggcc
     2581 gttgaacatg tccagctggt cgtgggtcat cagcccgtcg tacagcgggt tgtcgtagcc
     2641 gacctcgccg atacgccgca cgcggccctg cgacgggctg tagaaccaca cgccggtctc
     2701 gttcttgatg cggtcgatcg gcacgtgcag caggtgcgcc tcgccggcct cgttgggcgg
     2761 cgacaccgtc ttggtgaagg catacaggtt catgcccttg agcgagtcca ccgtgctctt
     2821 cacgttgttg tacatgtagg agagctcaag gtagctgcgc tcgatcacgt agctgccatc
     2881 ggcctgggtg gtggccgcgt tgatgtagcc gcggaaaccc ttggtgttgt agcgcatgat
     2941 gtggttccac atcacctcgg tgccgttctt gggaatcggg aacgggtaac cctgcgcggt
     3001 gcagcagaag ccgtagccgt tgtcggtcag ctcgacggtg gtcgcatttt ttttcgtggc
     3061 gtcgtagaac cactgcggat acgacgcgct gcggcgcgtc gggtacacga tcatcttgta
     3121 atcgggaaag gtcttgaaca ggttcttgta gccctcggtc agcacggcgt cgtactgcgc
     3181 catgttggcg gcggtgatgg tgtacagcgg cttgtcggcg gcgtaggggt tctcgccgcg
     3241 cgccatgtcc ttcttcggca ggccgccttc ccacttggga atggtgccgt ccttgttgcc
     3301 ggccacttcc gcgcccatag gcgtcagttc ggcgccgagc ttggcggctt cctccggcgt
     3361 gaccttggcc agcgccggcg cgcacagtgc cagcagcagc agaatgacgg ggattttttt
     3421 cattggattc tcctcgtggc tagaccgccg caggtgcagc tgcgttcccg ccgctgcgcg
     3481 gcaacaccac ccaggccagc gccggcagca gcaggatggc accgaacagg ttggtggtga
     3541 acatgaacag cagcagcgcg cccatgtcgc gctggaactg caggtcggaa aacagccagg
     3601 tgaacaccgc cacgatcagc gacagcgagg tgaacagcgc cgccttgccg gtgcgccgca
     3661 gcgcttcgaa gtaggcgtcg cgcaggccat agccttcttc caggtagcgc gcgatcacgc
     3721 tccagatgta gatgccggca tcgacgccga tgcccacgcc aaatgccgcc accggcagcg
     3781 tcgcgctttt catgccgatg tccagcgtgg ccatgccgcc gtaggtcagc agcgagcaca
     3841 gcaccagcgg ggtgatgatg caaatgacag cggcaaaaga gcggaacgag atcagcacga
     3901 agatcagcag cgtcagatgc acccagatga tcacggtgag ttcggtgcgc ttgatctctt
     3961 cgttggtcgc cgccatcacg ccgacgttgc ccgaggccag cgcgaaattc acgccttcgt
     4021 cctggtgttc ctggttgtag gccttggtcg cggcgacgat ccgcgagatg gtctcggcct
     4081 tgtggtcggt ggtgtagatc tgcaccggca tggccgagca gtcgaggttc tgcaggccgg
     4141 ccgtcgaagg caccgcgtgc gtggtctcgg acagcgccgc gcggttgcgc ggcagcacca
     4201 gccacttggg cgcgccttcc gacagtccct cgttgaggaa gcgcgccacc tgcggcagcg
     4261 acagtaccga ggccacgccc ggcgtgttct ggatatgcca ggcgtagttg tcgatggttt
     4321 ccatcagcgg cttctgcacg cagccgtact gcggcacctg cgcgatgacc ttgagcacgt
     4381 cgacgccgat gaagaagttg cgcgcgattt ccgcggagtc ctggttgtag cgcgagtcgg
     4441 gcttgagctc gggcacgccg gtctgcacat cgccgatgcg caggtccggg tatttccaga
     4501 ccgaccagcc gaaagcgatg gccgcgatga ccagcgccgg caccgcgctc cgcggcaggg
     4561 cgaggttggc cacgtgacgc cacaggcggt cgcccagttc ctg
//
LOCUS       DASZZC010000136         5313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1166471,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000136 DASZZC010000000
VERSION     DASZZC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166471"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..391)
                     /locus_tag="VHE37_03140"
     CDS             complement(23..391)
                     /locus_tag="VHE37_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34553.1"
                     /translation="MFRQARLWFGMITLLTGMSSYAQIETTPLDQAPVNAPRSVVVPP
                     VLPPHPVRPAIQAIANGGSGGLSYSGPSGAMNTSPDGASCAVTCSLAPREQYSIDCPA
                     GNTAYCQCDELPYAACRPQQ"
     gene            complement(484..975)
                     /locus_tag="VHE37_03145"
     CDS             complement(484..975)
                     /locus_tag="VHE37_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HVT34554.1"
                     /translation="MGKDKDKSAKIDIGIPKEQRKAIAEGLSRLLADEYTLYLKTHNF
                     HWNVTGPMFNTLHLMFETHYNESALAVDQVAERIRSLGYPAPGTYKQYAELSSIDETD
                     GVPEAMEMVRLLVKGHEACARTARGVFPAADAAQDEATADLLTQRMQAHEKTAWMLRS
                     LLE"
     gene            complement(1078..2025)
                     /locus_tag="VHE37_03150"
     CDS             complement(1078..2025)
                     /locus_tag="VHE37_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT34555.1"
                     /translation="MAEKKTILVTGAGGSLAKRVIARLHGKHHVVAVDFRKKVETDAG
                     IPSYKVDLYKRGFEDVFRDHKIDAVIHIGRIFTHESNRLNRYNANVLGTKRLLELSKK
                     YFVKQVVIHSTYFVYGASPYNPALLDEDAPLKATEVTQDLVDSVELESLANIYLWKHP
                     ELNITILRPCNVLGPGVKNSMSLLLSRPLVPVLVGFSPLMQFLHVDDMADALVAAYDQ
                     NRPGIYNVAPDDWVAYQEAVTQCGCKKLPIPSIPPLLPRTISNVLNWNAFFPPYLVNY
                     FKYPVILDGKLFRETFGWKPKRNLNDIFSYYRKNKILAA"
     gene            complement(2037..2888)
                     /locus_tag="VHE37_03155"
     CDS             complement(2037..2888)
                     /locus_tag="VHE37_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT34556.1"
                     /translation="MSYLDALKSLASVSPKEELFRRVVTPEIEEWIARSPKPVGSFGY
                     DPWGYNENAAKVGLGFAKLLYDKYFRVQATGLEHIPKQGRVLIIPNHSGQLPMDGVMI
                     GVALATNEHGPRIARAMIERFFPTVPWLGNTLSSWGGVIGDPLNCSKMLENDEAIIVF
                     PEGVRGSGKLYKDRYQLQRFGNGFMHLAMAHKTPIIPCGVVGCEETMPAVANIKPLAA
                     MLGLPYFPLAPLLPLPARVYLNFGEPMHFKGNVDHEEEVTARVEQVKAKIRELIELGL
                     SQRTKIY"
     gene            3038..3769
                     /locus_tag="VHE37_03160"
     CDS             3038..3769
                     /locus_tag="VHE37_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HVT34557.1"
                     /translation="MRGEDEIEVRRRALVRALTLGALSGGLGWNATAYASWFGRIPAR
                     IAEGKSIFDFSGDVTVNGSPVTSNTLIHAGDTIVTAENSHVVLVVGDTALTLRAKSRL
                     ETAGRDFLVNALRMVSGGMLAVFGKRSEALNISTPTATIGIRGTGVYTEVAPGQTYFC
                     TCYGTTQIASSADAGDAVQVTSKHHDAAKYILGDKQGGSRIVPAPFKNHTDVELMTLE
                     ALVGRKVPFAAPGLDYDAPRRDYEQ"
     gene            complement(3773..4045)
                     /locus_tag="VHE37_03165"
     CDS             complement(3773..4045)
                     /locus_tag="VHE37_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HVT34558.1"
                     /translation="MCRNIKTLFNFEPPATEEEIRASALQFVRKLSGFNAPSKANERA
                     FNLAVEQVTGAARVLIGSLVTLAPPRDRETEKARAILASARRFSRA"
     gene            complement(4055..4900)
                     /locus_tag="VHE37_03170"
     CDS             complement(4055..4900)
                     /locus_tag="VHE37_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34559.1"
                     /translation="MKIAAAALLCLPLVAQAYDGIVIGGEASAGYDTNITNAPGDAQP
                     IASSFAEAVVQAQQLYALGEYFSVLARGSLRAQEYKREDGLGNLKTGALLRLAWRPGM
                     DLYTPTVLLWSSASDWRYVSRLRDSEEYRGGVAWLQHLNTQFTARMSAGASARDGRSP
                     VFDLRTRHASFDLDWVPAQRLSLHGGWQFIRGDIVASTLPDVDIAAAAKAKAPDDAYD
                     ERPARVAYRIAARTRVATAGADYALTHALSLDAQVQYVRARPDYENAPYIRWIGSAGL
                     IWRLE"
     gene            complement(4897..>5313)
                     /locus_tag="VHE37_03175"
     CDS             complement(4897..>5313)
                     /locus_tag="VHE37_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT34560.1"
                     /translation="EEVERAARLAHLDSFIAHLPQGYDTVVGERGLKVSGGEKQRIAI
                     ARALLKNPAIMIFDEATSSLDSHSERAILGALREVAQHRTTLVIAHRLSTITDADEIV
                     VLEQGRVVERGTHESLLAAQGEYARLWQLQRREVPA"
BASE COUNT          847 a         1739 c         1773 g          954 t
ORIGIN      
        1 cgttcatgga cgcgccttca gctcactgct gcggacggca ggcggcgtaa ggcaactcgt
       61 cgcactggca gtacgcggta ttgccagcgg ggcagtcgat ggaatactgc tcgcgcggcg
      121 ccagcgaaca ggtcaccgcg cagctggcgc cgtcgggcga cgtgttcatc gcaccggacg
      181 ggccggaata tgaaagaccg ccactgccgc cgttggcgat ggcctggatc gccggccgca
      241 ccgggtgcgg cggcagcacc ggcggcacca ctaccgagcg cggcgcgttg accggcgcct
      301 ggtcgagcgg cgtggtttcg atctgcgcgt agctgctcat gccggtcagc agggtgatca
      361 tgccgaacca caggcgcgcc tgacgaaaca tgtgtacccc cggagtcgcc cacgaaactg
      421 ttgagcgacg gcatcctacc cgattgcgcc gtactgccgg gtggagcttt acacctaggc
      481 aaattactcg agcaggctgc gcagcatcca ggcagttttt tcatgtgcct gcatgcgctg
      541 ggtcagcagg tcggcggtgg cttcgtcctg cgccgcgtcc gcggccggaa acacgccgcg
      601 cgcggtcctg gcgcaggctt catgcccctt caccagcagg cgcaccattt ccatcgcctc
      661 gggcacgccg tcggtttcgt cgatcgaaga cagctcggca tactgcttgt aggtgcctgg
      721 tgccgggtag cccagcgagc ggatgcgctc ggccacctgg tccaccgcca gcgcggactc
      781 gttgtagtgc gtctcgaaca tcaggtgcag cgtgttgaac atcggcccgg tgacattcca
      841 gtggaagttg tgcgtcttca ggtacagggt gtactcgtcg gccagcaggc gcgacagccc
      901 ttcggctatg gccttgcgct gctccttggg aatgccgatg tcgatcttcg cggatttgtc
      961 cttgtccttg cccatcgtcg ttctccggtc cttggtggtc gatgcggaaa attctaggcg
     1021 caaggcgccc gccatgaaaa atgaattctt cctaagaccc gcatagccgc ggtcaattca
     1081 ggcggcgagg atcttgttct tgcggtagta gctgaagatg tcgttgagat tgcgcttggg
     1141 tttccagccg aaggtctcgc ggaacagctt gccgtcgagg atcaccgggt acttgaagta
     1201 gttcaccagg tagggcggga agaacgcgtt ccagttgagc acgttgctga tggtccgcgg
     1261 cagcagcggc ggaatcgacg ggatcggcag cttcttgcag ccgcactgcg tcaccgcctc
     1321 ctggtacgcg acccagtcgt cgggcgccac gttgtagatg cccggcctgt tctggtcgta
     1381 ggccgccacc agcgcgtcgg ccatgtcgtc cacgtgcagg aactgcatca gcggcgagaa
     1441 gcccaccagc accggcacca gcggccgcga cagcagcagc gacatgctgt tcttcacccc
     1501 cggtccgagc acgttgcacg ggcgcaggat ggtgatgttg agctcgggat gcttccacag
     1561 gtagatgttg gccaggcttt ccagttccac cgagtcgacc aggtcctgcg tcacctcggt
     1621 cgccttcagc ggtgcatcct cgtcgagcag cgccgggttg tacgggctgg cgccgtagac
     1681 gaagtaggtt gaatggatca ccacctgctt gacgaagtat ttcttcgaca gctccagcag
     1741 ccgtttggtg cccagcacgt tggcgttgta gcgattcagc cggttcgact cgtgggtgaa
     1801 gatgcggccg atgtggatca ccgcgtcgat cttgtggtcg cggaacacat cctcgaagcc
     1861 acgcttgtac aggtcgacct tgtacgatgg gatgccggcg tcggtctcca ccttcttgcg
     1921 gaagtcgacg gcgacgacat ggtgcttgcc gtgcagccgg gcgatgacgc gcttggccag
     1981 cgaaccgccg gcaccggtga cgaggatggt ttttttctcg gccatgatcg tcgtgctcag
     2041 tagattttgg tgcgctggga caggcccagc tcgatcagtt cgcggatctt cgccttgacc
     2101 tgctcgaccc gcgcggtgac ttcctcctcg tggtcgacat tgcccttgaa gtgcatgggc
     2161 tcgccgaaat tgaggtacac gcgggccggc agcggcagca gcggggccag cgggaaatac
     2221 ggcaggccca gcatggcggc cagcggcttg atgttcgcca ctgcgggcat ggtctcctcg
     2281 cagccgacta cgccgcaggg aataatcggc gtcttgtgcg ccatcgccag gtgcatgaag
     2341 ccgttgccga agcgctgcag ctggtagcgg tccttgtaca gcttgccgga accgcgcacg
     2401 ccttccggga acacgatgat ggcctcgtcg ttctccagca tcttgctgca gttgagcgga
     2461 tcgccgatca cgccgcccca ggacgacagc gtgttgccca gccacggcac cgtcgggaag
     2521 aagcgctcga tcatcgcgcg cgcgatgcgc ggcccgtgct cgttggtggc cagcgccacg
     2581 ccgatcatca cgccgtccat cggcagctgc cccgagtggt tggggatgat cagcacgcga
     2641 ccctgtttcg ggatgtgctc cagcccggtg gcctgcacgc ggaaatactt gtcgtagagc
     2701 agcttggcaa agcccagccc gaccttggcc gcgttctcgt tgtagcccca cgggtcatag
     2761 ccgaagctgc ccaccggctt gggcgagcgc gcgatccatt cctcgatctc gggcgtgacc
     2821 acgcggcgga acagttcctc cttcgggctc acgctggcca ggctcttcag cgcatccagg
     2881 tacgacatgg cgttgactgc tccccgcagc gaccacggtt cttgttggcg gcaaggtatg
     2941 cgaaaacgcc cggcgcggga agtggcgtga tgcgcagcgc ttgccgcatg cgccgcggca
     3001 ggtacgatgg acgctacata gaccggggag tgccgccatg cgcggggaag acgagatcga
     3061 ggtgcgccgg cgcgcgctgg tgcgtgcgct gacgctgggt gcgctgtcgg gtgggctggg
     3121 ctggaacgcc accgcgtatg cctcgtggtt cgggcgcatc cccgcgcgca tcgccgaggg
     3181 caaatccatc ttcgattttt ccggcgacgt caccgtcaac ggctcgccgg tgaccagcaa
     3241 cacgctgatc catgccggcg acaccatcgt caccgccgag aacagccatg tcgtgctggt
     3301 ggtcggtgac accgcgctca ccctgcgcgc gaaatcgcgc ctggagaccg ccggtcgcga
     3361 tttcctggtc aacgccttgc gcatggtcag cggcggcatg ctggcggtgt tcggcaagcg
     3421 cagcgaggcg ctgaacatca gcacgcccac cgccaccatc ggcatccgcg gcaccggcgt
     3481 gtacaccgag gtagcgccgg gccagaccta tttctgcacc tgctatggca ccacccagat
     3541 cgcctcgtcg gccgatgccg gcgacgcggt gcaggtcacc tcgaagcatc acgacgcggc
     3601 caagtacatc ctgggcgaca aacagggcgg cagccgcatc gtacctgcgc cgttcaagaa
     3661 ccacaccgac gtggaactga tgacgctgga agcgctggtc gggcgcaagg tgccttttgc
     3721 agcgccgggc ttggactacg acgcgccgcg gcgcgactac gagcagtaag cgtcaggccc
     3781 ggctgaagcg cctggcggag gccagtatcg cccgcgcttt ttcggtctcg cggtcgcgcg
     3841 gcggcgccag cgtgaccagc gagccgatca gcacgcgcgc ggcacccgtc acctgttcca
     3901 ccgccagatt gaacgcccgc tcgttggctt tcgacggcgc gttgaagccg gacagcttgc
     3961 gcacgaactg cagcgcggac gcgcggattt cctcttcggt cgccggcggc tcgaagttga
     4021 acagcgtctt gatgttgcgg cacatgggcg gctcctactc gaggcgccag atcagcccgg
     4081 cactgccgat ccagcggatg tacggcgcgt tctcataatc gggccgcgcg cggacgtact
     4141 gcacctgcgc atcgagcgac agcgcgtgcg tgagcgcgta gtcggcgccg gcggtcgcta
     4201 cccgcgtgcg cgccgcgatg cgataggcca cgcgcgcggg ccgctcatcg taggcatcgt
     4261 ccggcgcctt ggccttggcg gcggcggcaa tgtcgacatc gggcaaggtg ctggcgacga
     4321 tgtcgccgcg gatgaactgc cagccgccgt gcagcgacag ccgctgcgcc ggcacccagt
     4381 caaggtcgaa cgaagcatgg cgcgtgcgca ggtcgaacac cgggctgcgc ccgtcgcgtg
     4441 cggatgcgcc cgctgacatg cgcgctgtga actgcgtgtt caggtgctgc agccaggcca
     4501 cgccaccgcg atattcctcg ctgtcgcgca ggcggctgac gtagcgccag tcggacgcgc
     4561 tgctccacaa caggaccgtg ggcgtgtaca ggtccattcc cgggcgccag gccagccgca
     4621 gcagcgcgcc ggtcttcaga ttgcccagac cgtcttcgcg cttgtactcc tgcgcgcgca
     4681 ggctgccgcg cgccagcacg ctgaagtatt cgcccagtgc gtacagctgc tgcgcctgca
     4741 ccacggcttc ggcgaagctg ctggcaatgg gctgcgcatc tcccggcgcg ttggtgatat
     4801 tggtgtcgta gccggcactg gcttcgccgc cgatgacgat gccgtcgtag gcctgcgcca
     4861 ccaacggcag gcagagcaat gcggcggcgg cgatcttcac gccggcactt cgcgccgctg
     4921 cagctgccac aggcgcgcgt attcgccctg cgcggcgagc agcgactcgt gggtgccgcg
     4981 ttcgaccacg cggccctgtt ccagcaccac gatctcgtcg gcatcggtga tggtcgacag
     5041 ccggtgggcg atgaccagcg tggtgcggtg ctgcgccacc tcgcgcagcg cgccgaggat
     5101 ggcgcgctcg ctgtgcgagt ccagcgacga ggtggcttcg tcgaagatca tgatcgccgg
     5161 attcttcagc agcgcccgcg cgatggcgat gcgctgcttc tcgccgccgg aaactttcag
     5221 cccgcgctcg ccgacgacgg tgtcgtaacc ctgcggcagg tgcgcgatga agctgtccag
     5281 gtgcgcgagc cgcgcggcgc gctccacttc ctc
//
LOCUS       DASZZC010000137         5918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_973648,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000137 DASZZC010000000
VERSION     DASZZC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973648"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..793)
                     /gene="xth"
                     /locus_tag="VHE37_03180"
     CDS             complement(26..793)
                     /gene="xth"
                     /locus_tag="VHE37_03180"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVT34561.1"
                     /translation="MKIATWNVNSLRVRLQHLTDWLAREQPDVVGLQELKLQDEQFPH
                     DALRELGYHAVCNGQKSYNGVAILSKTPAQDVTRDMPGFDDPQKRVIGATINGVRVID
                     VYVVNGEEVGSQKYEYKLRFLAALHEYLKQEVARHPRLVVLGDYNIAPADADVHHPDK
                     NVGVVMASEPERQAFSDLVALGLSDCFRLFPQPEKAYTWWDYRYFAFNKNLGWRIDHL
                     LASKELAAKCLNCRIDVAPRKLERPSDHAPVIAEYDV"
     gene            complement(833..955)
                     /locus_tag="VHE37_03185"
     CDS             complement(833..955)
                     /locus_tag="VHE37_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34562.1"
                     /translation="MKNVLRGFVVLMVRALKFVTGALTRKFVICSGRIAPRHRA"
     gene            996..1370
                     /locus_tag="VHE37_03190"
     CDS             996..1370
                     /locus_tag="VHE37_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVT34563.1"
                     /translation="MKVSEVMARNPRTIRCGELVQKAAELLAQEDIGGLPVQKDDRLL
                     GMITDRDIVVRVVAGHRKPEETPVEEVVSEGVKYCFEDDDAASVANNMDQLLVRRLPV
                     VNRDKRLVGIVSIEDIRPRANT"
     gene            1472..2509
                     /locus_tag="VHE37_03195"
     CDS             1472..2509
                     /locus_tag="VHE37_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34564.1"
                     /translation="MLVSAALGLLAATAGCSHPPPCTAGDRDCARAAIRNHPARRIEF
                     WNRMLSRPLVQRIGPAPQVLLDYLVLDNTAQGVPDHPHAPRLSAEFLADLHDAFEQLP
                     PAVTDPLARRLAGIYLVDGLGRSAYTEDVLDESGEPVAAVIVLDAQMLSQRKANAWAS
                     WKENTPFTPEDGWALHAEIERPEQDNERSAIQYILLHELGHAISVGRHLHPAGSRPPP
                     KTTGDFAFFELSWKVLPQGEYESLFSADFPQRRDVTYYFGARLAASQMLPVYQALQRT
                     NYPTLYAATSPGDDFAESFANYVHVVMLHRPYTIHIAHAGEPVHDFGACWNTARCRAK
                     RRLIEQLLDAG"
     gene            complement(2528..3970)
                     /gene="tldD"
                     /locus_tag="VHE37_03200"
     CDS             complement(2528..3970)
                     /gene="tldD"
                     /locus_tag="VHE37_03200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HVT34565.1"
                     /translation="MTNTLELVRDSMLAPAGLDESRLQQVLSGLMKPGIDAADLYFQH
                     ALTQSWSLEDGIVKSGSHHVEQGVGVRALSGDKQGLAYTDDLILPALTDAAGAAGAIV
                     RAGQQGEVAAWRRPSFKALYPSLNPIDTSSTGLTLDLLRRADAAARAADARVIQVNVS
                     LAANHESVLVAAADGTLAADVRPLVRMDVSVIVEHNGRREQGHAGGGGRGGIAEFFEH
                     EQPENYAREAVRQALVQLEAVPAPAGSMTVVLGPGWPGVLLHEAVGHGLEGDFNRKGT
                     SAFSGRVGQKVASELCTIVDDGTLLSRRGSLSLDDEGTPTQCTTLIENGVLRGYIQDK
                     LNARLMGVAATGNGRRESYAHLPMPRMTNTYMLPGKSDPAEIIASVEKGLYAVNFGGG
                     QVDITSGNFVFSASEAYLIENGKVTRPVKGATLIGNGPEALGRVSMVGNDLKLDTGVG
                     VCGKDGQSVPVGVGQPTLKLDALTVGGTQG"
     gene            complement(4019..4843)
                     /locus_tag="VHE37_03205"
     CDS             complement(4019..4843)
                     /locus_tag="VHE37_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HVT34566.1"
                     /translation="MKAAVLQMNSGDDLAANLAQAQSLLEQAQAQGCKLAVLPENFAF
                     MGAHERDKLRHAESAGGGAIQDFLSRAAARLRLWIVGGTAPLAVAHTPDKVYAASLVY
                     DDRGACVARYDKIHLFDVQVPTAGGAQNYRESASIEAGVPRAQTIATPAGILGLSVCY
                     DLRFPELYRALSALGVEVFAVPSAFTAATGAAHWSALLRARAIENQAYVLAPGQCGTH
                     PGGRQTWGHSLIVDPWGIVLCELGDAPGIGVAELDFERLAQVRTTFPALRHRRLSS"
     gene            complement(4859..>5918)
                     /locus_tag="VHE37_03210"
     CDS             complement(4859..>5918)
                     /locus_tag="VHE37_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HVT34567.1"
                     /translation="LRDTQVSLQMVEHGWKAHLAGADADGDIGYADDDAGGKLGAKLA
                     RLSVEGQPAGDTDKTPSAARAPLEPGNWPALDLSCAQCQLDGADLGALRLVSAHVAGG
                     QRVDEFSLSGGQTEAHASGIWLRQNDLSSAQLKIDLSSANFGGLVKALGYTQNVTARS
                     SRFGGEFEWTPAAGGLEWEQASGKLALEFKKGNLKAVQPGAGRMLGLLNFYALPRRLT
                     LDFHDVVDAGLAFDSISGDFDFGGGNASTSNLKIEGPSLRMEMKGRVGLAARDYDEHV
                     TVVPDISSGVTLGAALLGGPALGALAFVAQEVLDKPISEATQLQYRVTGSWDNPDIKR
                     VEGKAEKTDLPKPAKPGH"
BASE COUNT          919 a         2058 c         1967 g          974 t
ORIGIN      
        1 gaaaagggag tcttttcaca gtgactcaaa cgtcatattc ggcgatgacc ggggcatggt
       61 cggaagggcg ctccagcttg cgcggtgcaa catcgatgcg acagttcagg cacttggccg
      121 ccaactcctt cgatgccagc aggtggtcga tgcgccagcc caggttcttg ttgaaggcaa
      181 agtagcggta atcccaccag gtgtaggcct tttccggctg cggaaacagg cggaagcagt
      241 cggacagccc cagcgcgacg aggtcgctga aggcctggcg ctccggctcc gacgccatga
      301 ccacgccgac gttcttgtcc gggtgatgca catcggcgtc ggccggcgcg atgttgtagt
      361 cgcccagcac caccagcctg gggtggcgcg ccacttcctg cttcaggtac tcgtgcaggg
      421 ccgccaggaa gcgcagcttg tactcgtatt tctgcgagcc gacttcctcg ccgttgacca
      481 cgtaaacgtc gatcacacgc acgccgttga tggtggcgcc gatcacccgc ttctggggat
      541 cgtcgaaacc gggcatgtcg cgggtgacgt cctgcgccgg cgttttcgac agtatggcca
      601 cgccgttgta gctcttctgg ccattgcaca cggcgtggta cccgagttcg cgcagtgcgt
      661 cgtgcggaaa ctgctcgtcc tgcagcttga gctcctgcag gccgaccacg tcgggctgct
      721 cgcgggccag ccagtcggtc aggtgctgca ggcgcacgcg caggctgttg acgttccagg
      781 tggcgatttt cacgacaggt ttttccgctt tcgttgggat tgcagatttt gctcaggccc
      841 ggtggcgcgg cgcaatgcga ccggagcaaa tcacgaactt tcgcgttaac gctccggtca
      901 caaatttgag cgcgcgcacc atcagaacca caaagccgcg cagcacattc ttcatcggct
      961 gcggcgccgc gtggccgtac ccaggagcca ggagcatgaa agtttcggaa gtgatggcgc
     1021 gaaacccgcg gaccatccgc tgcggtgaat tggtgcagaa ggcggcggaa ttgctggcgc
     1081 aggaagacat cggcggactg ccggtgcaga aggacgaccg cctgctcggc atgatcaccg
     1141 accgcgacat cgtggttcgc gtggtggccg gtcaccgcaa gccggaggaa acgccggtcg
     1201 aggaagtggt cagcgaaggc gtgaagtact gcttcgagga tgacgacgcc gccagcgtgg
     1261 ccaacaacat ggaccagttg ctggtgcggc ggctgccggt ggtgaaccgg gacaagcgcc
     1321 tggtcggcat cgtctccatc gaggatatcc gtccgcgcgc caacacctga cgccggacgc
     1381 gcaaccagcg cgccggatgg cgtaacctgc gcgcgcggcc aggccggccg catgccggtc
     1441 atgccatcgc ttgccgccgg gaacgcgaca tgtgcttgtg tctgcggcgc tgggcctgct
     1501 cgcggccacg gcgggctgca gccatccgcc gccctgcacg gcgggcgacc gcgactgcgc
     1561 ccgtgccgcc atccgcaatc acccggcccg gcgcatcgag ttctggaacc gcatgctgtc
     1621 gcggccgctg gtgcagcgca tcggcccggc gccgcaggtg ctgctcgatt acctggtgct
     1681 ggacaacacc gcgcagggcg tacccgacca tccgcacgca ccgcggctgt ctgcggaatt
     1741 cctcgccgac ctgcacgatg ccttcgagca gctgcccccg gcggtcaccg atccgctggc
     1801 ccgccggctg gccggcatct acctggtgga tggcctcggc cgcagcgcct ataccgaaga
     1861 cgtgctcgac gagagcggcg agccggtggc cgcggtcatc gtgctcgatg cccagatgct
     1921 gtcgcagcgc aaggccaatg cctgggccag ctggaaggaa aacacgccct tcaccccgga
     1981 ggatggctgg gcactccatg ccgagatcga gaggcctgag caggacaacg agcgcagcgc
     2041 catccagtac atcctgctgc acgaactggg gcacgcgatc tccgtgggcc gtcacctgca
     2101 ccccgcgggg agccgcccgc cgccgaagac caccggcgac ttcgcattct tcgagttgtc
     2161 ctggaaagtc ctgccacagg gcgaatacga atccttgttc tcggcggatt tcccgcagcg
     2221 gcgtgacgtg acgtattact tcggcgcgcg cctggcggcg agccagatgc tgccggtcta
     2281 ccaggccctg cagcgcacca actaccccac gctgtacgcc gccacctcgc cgggcgatga
     2341 cttcgccgag agctttgcca actacgtgca cgtggtcatg ctgcaccggc cgtacaccat
     2401 ccacatcgcc catgccggcg agccggtcca cgatttcggc gcatgctgga acaccgcgcg
     2461 ctgtcgggcc aagcggcgcc tgatcgaaca gctgctggat gccggctgag gcagcaggcg
     2521 gtgcggttca gccctgcgtg ccgcccacgg tcagcgcatc gagcttgagc gtcggctggc
     2581 ccacgcccac cggcacgctc tggccatcct tgccgcagac tcccacgccg gtatcgagct
     2641 tgagatcgtt gccgaccatg ctcacgcggc cgagcgcctc ggggccgttg ccgatcagcg
     2701 tggcgccctt caccggccgc gtgaccttgc cgttctcgat caggtacgcc tcgctggcgg
     2761 agaagacgaa attgcccgaa gtgatgtcga cctggccgcc gccgaagttg accgcgtaga
     2821 ggcctttctc caccgaggcg atgatctccg ccggatcgct cttgcccggc agcatgtagg
     2881 tgttggtcat gcgcggcatc ggcaggtgtg cgtagctctc gcgccggccg ttgccggtgg
     2941 cggctacgcc catcagccgc gcgttgagct tgtcctggat gtagccgcgc agcacaccgt
     3001 tctcaatcag agtggtgcac tgcgtcggcg tgccctcgtc gtccagcgac agcgaaccgc
     3061 ggcgcgacag cagcgtgccg tcatcgacga tcgtgcagag ttccgacgcc accttctggc
     3121 cgacgcgccc ggaaaacgcc gaggtgccct tgcggttgaa gtcgccttcg agcccatggc
     3181 ccacggcctc gtgcagcagc acgccgggcc agcccgggcc tagcaccacg gtcatcgaac
     3241 ccgcgggcgc cggcactgct tcgagctgca ccagcgcctg gcgcaccgct tcgcgcgcgt
     3301 aattttccgg ctgctcgtgc tcgaagaatt cggcgatgcc gccgcggcca ccgccgccgg
     3361 cgtggccctg ctcgcgccgg ccgttgtgtt cgacgatcac cgacacgtcc atgcgcacca
     3421 gcgggcgcac atccgctgcc agcgtgccgt ccgccgccgc caccagcacg ctctcgtggt
     3481 tggccgccag cgacacgttg acctggatca cgcgggcatc ggccgcgcgg gcggcggcgt
     3541 cggcgcgtcg cagcaggtcc agcgtgagcc cggtgctgct ggtgtcgatg ggattcagcg
     3601 atggatacag cgccttgaac gaaggccggc gccaggcggc gacttcgccc tgctgtccgg
     3661 cgcgcacgat cgcgccggcg gcgccggccg catcggtcag cgccggcagg atgaggtcgt
     3721 cggtatacgc gaggccctgc ttgtcgcccg agagcgcgcg cacacccacg ccctgttcga
     3781 cgtggtggct gccggacttg acgatgccgt cctccagcga ccagctctgc gtgagcgcgt
     3841 gctggaaata aagatcggcc gcgtcgatgc ccggcttcat caacccggac agcacctgct
     3901 gcagccgcga ctcgtcgagg ccggcgggcg ccagcatgga atcgcggacc aactcaagag
     3961 tattcgtcat ggatatagcc taagaccgga atcgcctgcg ctcgttcccg ccgccgcttc
     4021 aggacgacag gcggcggtga cgcagggcgg gaaaggtggt gcgcacctgg gccaggcgct
     4081 cgaagtcgag ttcggccacg ccgatgccgg gcgcgtcgcc gagttcacac agcacgatgc
     4141 cccaggggtc gacgatcagc gagtggcccc aggtctgccg tccgccaggg tgcgtgccgc
     4201 actgccccgg tgccagcacg taagcctggt tctcgatggc gcgcgcacgc agcagcgccg
     4261 accagtgcgc ggcgccggtg gcggcggtga aggcggacgg caccgcgaac acctccacgc
     4321 ccagcgcaga cagggcgcgg tacaactcgg gaaagcgcag gtcgtagcac accgacaggc
     4381 cgaggatgcc cgcgggcgtg gcgatggtct gcgcgcgcgg cacgccggcc tcgatgctgg
     4441 ccgactcgcg gtagttctgc gcgccgccgg ccgtgggcac ctgcacgtcg aacaggtgga
     4501 tcttgtcgta gcgcgccacg caggcgccgc ggtcgtcgta caccaggctg gcggcgtaga
     4561 ccttgtcggg tgtgtgcgcc accgccagcg gcgcggtgcc gccgacgatc cacagccgca
     4621 gccgcgcggc ggcacgcgac aaaaaatcct ggatcgcgcc gccgccggcg ctctcggcat
     4681 ggcgcaactt gtcgcgctcg tgcgcgccca tgaaggcgaa gttttccggc agcaccgcca
     4741 gcttgcagcc ctgcgcctgt gcctgctcga gcagcgactg tgcctgcgcc aggttggcgg
     4801 ccaggtcatc gcccgaattc atctgcagca ccgcggcttt catgcggaca ttgtcgtctt
     4861 aatgcccggg ctttgcaggc ttcggcagat cggttttctc ggccttgcct tcaacgcgct
     4921 tgatgtcggg attgtcccag ctgccggtca cccggtactg cagctgggtg gcctcgctga
     4981 tcggcttgtc cagcacttcc tgcgcgacga aggccagcgc gcccagcgcc ggtccgccga
     5041 gcaatgccgc gcccagggtg acgccggagc tgatgtccgg caccacggtt acgtgttcgt
     5101 cgtagtcgcg cgcggccagg cccacgcgcc ctttcatctc catgcgcagc gacgggcctt
     5161 cgatcttgag gttggaggtg ctggcgttgc cgccgccgaa atcgaaatcg ccgctgatgc
     5221 tgtcgaaggc cagccccgca tcgaccacgt cgtggaaatc cagcgtgagc cggcgcggca
     5281 gcgcgtagaa attgagcagg ccaagcatgc gcccggcacc gggctgcacc gccttgaggt
     5341 tgcccttctt gaactccagc gccagcttgc cgctggcctg ctcccattcc aggccgccgg
     5401 ccgccggcgt ccactcgaac tcgccgccga acctgctgct gcgcgcggtg acgttctgcg
     5461 tgtagcccag agccttgacc agcccgccga agttggcgct gctcaggtcg atcttgagct
     5521 gcgcgctcga caggtcgttc tgccgcagcc agatgccgct ggcgtgggct tcggtctggc
     5581 cgccgctgag cgagaactca tcgacgcgct gtccgcccgc aacatgcgcg ctgaccaggc
     5641 gcagtgcacc caggtccgct ccatcgagct ggcactgcgc gcaggacagg tccagcgccg
     5701 gccagttgcc cggctccagc ggcgcgcgcg ccgctgacgg cgtcttgtcg gtgtcgccgg
     5761 cgggctggcc ttccaccgac aggcgcgcca gtttcgcgcc cagcttgccg cccgcatcgt
     5821 cgtcggcgta gccgatgtcg ccatcggcat cggcgccggc caggtgcgcc ttccagccat
     5881 gctcgaccat ctgcagcgac acctgtgtgt cgcgcagg
//
LOCUS       DASZZC010000138         6770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_846318,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000138 DASZZC010000000
VERSION     DASZZC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846318"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VHE37_03215"
     CDS             <1..155
                     /locus_tag="VHE37_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVT34568.1"
                     /translation="ANELGLSYSRFINGLTKAGVALDRKVLADMAIHDKAAFAQLVAQ
                     AKAQLA"
     gene            234..1244
                     /locus_tag="VHE37_03220"
     CDS             234..1244
                     /locus_tag="VHE37_03220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HVT34569.1"
                     /translation="MTTTPEQLLESASAQIAAAADARALEQLRVDLLGKKGQVTELLK
                     QLGALPAEERKTHGARVNRVKEQIAALLDERGAVLAGAALATQLAAEAVDVTLPGRGE
                     STGGLHPITRTIRRIERLFNDMGFATREGPEIEDDFHNFTALNFPELHPARAMHDTFF
                     VKNGAGVLRTHTSPVQVRAMQALVQSGAQPPLRIIAPGRVYRCDSDRTHSPMFHQVEG
                     LYVAEGVSFADLQYDLKTFLTRFFEREAEVRFRPSFFPFTEPSAEVDIRDEKGRWLEV
                     LGCGMVHPNVFTAVGLDPERYTGYAFGMGVERFAMLRYGVDDLRLFFNNDVRFLAQFR
                     DA"
     gene            1254..3611
                     /gene="pheT"
                     /locus_tag="VHE37_03225"
     CDS             1254..3611
                     /gene="pheT"
                     /locus_tag="VHE37_03225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HVT34570.1"
                     /translation="MKLSYNWLREWAAVPATASELTARITLAGLESEAEPMTGEIPSK
                     VVVGLIVKAEKLPQADRLQICEVDVGSGTNLQIVCGAPNARAGLHAPCALAGARLPGG
                     TEIKQGRIRGVESHGMLCSAKELGLSDRSEGLLELDSDSKTGTPIEQYLSFNDDILNL
                     EFTPNRGDAVSVQGLAREVAAIYNLQLKRPNMLAPVVVGHTQYKVEIEDLADCPLYSG
                     RVVSKLNPKARTPDWMREKLRRSGIRAISPVVDVTNYVMLELGQPLHAFDTTKLSGAV
                     RVRRARDGESLTLLNGETVQLGARDLVIAHDKGVLGLAGVMGGQDSGVNETTTSVFLE
                     SACFSPLAVAGTGRRHKISSDALYRFERGVDPGAQRAALDRATQLLLQISGGEVHPVS
                     QTGRAPEQVTVRLRHARVKQLLGHDIPAKEIEALLARLSITVRQESGGAWTARVPSFR
                     TDLRIEADLIEEIARIYGYDRIPAQPYRAALAPEPQPERLRRLDALRDTLVARGWQEA
                     VTLSFVEPRVQQLITPDVAGIALDNPLAEQLAVMRTSLWPGLIAAWLYNQQRQQKRTR
                     LFEVGVCFADVNGAVIETPRIAGLAAGEAVSEQWGMPTRATDFHDIKGDAAALLPADC
                     EFVAAAHPALHPGRSARVLRNGAHAGWIGALHPAVAAKLDLPESPFLFELDWTAIREV
                     ALPHANTPSEFPSSRRDLAVVVPEVVTAQQLSTLARRVGGNSLQKVIVFDIYRGKALG
                     DGFKSVAFGLIFNDYSRTLNVEEIDAAVAAITQALSTELQAAIRQ"
     gene            3623..3916
                     /locus_tag="VHE37_03230"
     CDS             3623..3916
                     /locus_tag="VHE37_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HVT34571.1"
                     /translation="MALTKAELAEALFEELGLNKREAKEFVDLFFELIRGRLEQGEEV
                     KLSGFGNFELRSKNQRPGRNPKTGEEIPISARRVVTFRPGQKLRLRVETDAAG"
     gene            3903..4307
                     /locus_tag="VHE37_03235"
     CDS             3903..4307
                     /locus_tag="VHE37_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVT34572.1"
                     /translation="MRPAEQKSLPLPEIPRKRYFAIGEVSELCDVKPHVLRYWEQEFP
                     QLKPVKRRGNRRYYQHDDVLMVRRIRSLLYDQGFTIGGARQRLKETPKQIPLAAGNGS
                     VQAKPTRGGVRRAVEIRTLRNELESLLKLFPQ"
     gene            4367..4443
                     /locus_tag="VHE37_03240"
     tRNA            4367..4443
                     /locus_tag="VHE37_03240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4401..4403,aa:Pro,seq:ggg)
     gene            complement(4459..4956)
                     /locus_tag="VHE37_03245"
     CDS             complement(4459..4956)
                     /locus_tag="VHE37_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34573.1"
                     /translation="MNQTEGHGSSPEAAEDPRASLVNQAARLLQDSQLDAAQELLAQA
                     LQRFPRYARAHQLQANLAGRRGHWRTSLEHWRIFLKLKPAHLAGYVSALACAAQLDNS
                     LAAEEVAAMAPGEVAARVDFQMRVRLPVLRLRQQVDAARTLIGALDEATLDAAGSVEA
                     AVTQC"
     gene            complement(4986..5816)
                     /locus_tag="VHE37_03250"
     CDS             complement(4986..5816)
                     /locus_tag="VHE37_03250"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HVT34574.1"
                     /translation="MAPLILVRRCLALLLLCLCWPAHAQTYSPMAEWQYSSGIALEPR
                     FVREPPKWEVQLGGGTSYLPRFEGSNRYRYQPGPMIEIRHRDDWFFSLGEGLGYNILS
                     ERYYRAGFAVSYDLGRNDQKDPALSGLGSIKAAPEGKLFYEYVSFPAVIRLNGRHAWG
                     GHNGWIGDISLYSPIAGNKKFFVFVGPSATFADSTYMHHYFGVNAAQSARSGYRVYDP
                     GGGLKDARFGATAVWFMTEHWMLNTLFAAEWILGPARDSPIVERTQGYTGNLYFGYNF
                     "
     gene            complement(5853..6467)
                     /locus_tag="VHE37_03255"
     CDS             complement(5853..6467)
                     /locus_tag="VHE37_03255"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT34575.1"
                     /translation="MRLTFALLLFAPCVVALAIAAPDQAIRDTNTQFLAQIDQHRDEF
                     RRDRARFDRMVDQVVVPRFDIPYIGRLVLGREWKSASDAQRSRFLAAFKNSLVHEYAH
                     ALLDYGDGLQEQWHPLQAAPGAGDVTVHMDLSSPQAAPLQVGFAAHRVNDDWKVYDVT
                     VNGISLAASFRAQFAVEIALYGLDGVIQRLESGGPPLQIHPGTG"
     gene            complement(6578..>6770)
                     /locus_tag="VHE37_03260"
     CDS             complement(6578..>6770)
                     /locus_tag="VHE37_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34576.1"
                     /translation="ATGMVSANSLALLSMGFTDEVADWTPRRRPVFGRCAHQGHRTGR
                     KCLHRTGLMDVIIYILVIY"
BASE COUNT         1207 a         2179 c         2262 g         1122 t
ORIGIN      
        1 ccgccaacga gctgggactg tcgtacagcc gatttatcaa tgggcttacc aaggccggcg
       61 tcgccctcga tcgcaaggtc ctcgcggaca tggcgatcca tgacaaggcg gcgttcgcgc
      121 agttggtggc ccaggccaaa gcacagctcg cttaatcagc tccttcgacg taacgaagca
      181 cccgaaggga gcggcgagcg ccgctccctt ttttcgtgtc cggtatctga gccatgacaa
      241 ccacgcctga acaactgctg gaaagcgcca gcgcgcagat cgctgccgcc gccgatgccc
      301 gcgcgctgga acagctgcgc gtcgacctgc tgggcaagaa agggcaggtg accgaactgc
      361 tcaagcagct cggcgcgctg ccggccgagg agcgcaagac gcacggcgcg cgggtcaacc
      421 gcgtgaagga gcagatcgcc gcgctgctcg acgagcgcgg cgccgtgctg gccggcgcgg
      481 ccctggcgac gcagctcgct gcggaagctg tcgacgtaac gctgccgggc cgcggcgagt
      541 cgaccggcgg gctgcatccg atcacgcgca ccatccggcg catcgagcgg ctgttcaacg
      601 acatgggctt cgccacgcgc gaagggccgg agatcgagga cgacttccac aatttcaccg
      661 cgctgaattt cccggagctg cacccggcgc gcgccatgca cgacactttc ttcgtcaaga
      721 acggcgccgg cgtgctgcgc acgcatacca gcccggtgca ggtgcgcgcg atgcaggcgc
      781 tggtgcagtc cggcgcgcag ccgccgctgc gcatcatcgc gcccgggcgc gtgtaccgct
      841 gcgattccga ccgcacgcac agcccgatgt tccaccaggt ggaaggcctg tacgtggccg
      901 aaggggtctc gttcgccgac ctgcagtacg acctcaagac cttcctcacg cgcttcttcg
      961 agcgcgaggc cgaggtgcgc ttccgcccgt cattcttccc gttcaccgag ccgtcggcgg
     1021 aagtggacat ccgcgacgag aaaggccgct ggctcgaggt gctcggctgc ggcatggtgc
     1081 atcccaacgt cttcaccgcg gtgggtctcg acccggagcg ttacaccggc tacgccttcg
     1141 gcatgggcgt ggagcgcttc gccatgctgc gctacggcgt ggatgacctg cgcctgttct
     1201 tcaacaacga cgtgcgcttc ctcgcacagt tccgcgacgc gtaagggacc gccatgaaac
     1261 tttcctacaa ctggctgcgt gagtgggcgg ccgtcccggc aacggcttcg gaactgaccg
     1321 cgcgcatcac gctggctggc ctcgaatcgg aagccgagcc gatgacgggc gagattccgt
     1381 cgaaggtggt ggtcgggctg atcgtcaagg ccgagaagct gccgcaggcc gaccgcctgc
     1441 agatctgcga agtcgacgtg ggcagcggca cgaatctgca gatcgtctgc ggcgcgccga
     1501 acgcgcgcgc ggggttgcac gcaccgtgtg cgctggccgg cgcgcgcctg cccggcggca
     1561 ccgagatcaa gcagggacgc atccgtggtg tcgagtccca cggcatgctg tgctcggcca
     1621 aggaactggg cttgtccgac cgctcggaag gcctgctgga actggacagc gactcgaaga
     1681 ccggtacgcc gatcgagcag tacctgtcat tcaacgacga catcctcaac ctcgagttca
     1741 cgcccaaccg cggcgacgcg gtgtcggtgc agggactcgc gcgcgaagtg gcggccatct
     1801 acaacctgca gctcaagcgc ccgaacatgc tggcgccggt ggtggtcggc catacgcagt
     1861 acaaggtgga gatcgaagac ctggccgatt gtccgctgta cagcgggcgc gtggtcagca
     1921 agctcaaccc gaaggcgcgc acgccggact ggatgcgcga aaagctgcgg cgcagcggca
     1981 tccgtgcgat cagcccggtg gtcgacgtca ccaactacgt gatgttggaa ctgggccagc
     2041 cgctgcacgc cttcgacacg acaaagctgt ccggtgcggt gcgcgtgcgc cgcgcgcgcg
     2101 acggtgaatc gctcacgctg ctcaacggcg aaaccgtgca gctgggcgcg cgcgacctgg
     2161 tgatcgcgca cgacaagggc gtgctcggtc tggccggcgt gatgggaggg caggacagcg
     2221 gcgttaacga aaccacgacc agcgtgtttc tcgaatcggc ctgcttctcg ccgctggcgg
     2281 tggccggcac cggacgccgg cacaagatca gctccgatgc gctgtatcgt ttcgagcgcg
     2341 gcgtcgatcc gggtgcgcag cgcgcggcgc tggaccgcgc gacacagttg ttgttgcaga
     2401 tcagcggcgg cgaagttcac ccggtatcgc agaccggccg cgcgcccgag caggtgaccg
     2461 tgcgcctgcg ccacgcgcgc gtgaagcagc tgcttggcca cgacatcccg gcgaaggaga
     2521 tcgaagcgct gctggcgcgg ctctccatca cggtgcgcca ggaaagcggc ggcgcctgga
     2581 ctgcgcgcgt gccgagcttc cgcaccgatc tgcgcatcga ggccgacctg atcgaagaga
     2641 tcgcgcgcat ctatggctat gaccgcatcc cggcccagcc ttatcgcgca gcgctggctc
     2701 cggagccgca gcctgagcgt ctgcgtcgcc tcgatgcgct gcgtgacacg ctggtggcgc
     2761 gcggctggca ggaggcggtg acgctgtcat ttgtcgagcc gcgcgtgcag caactcataa
     2821 ccccggacgt cgccggcatc gcgctggaca atccgctggc agagcagttg gcggtgatgc
     2881 gcacctcgct gtggcccgga ctgatcgccg cctggctgta caaccagcag cggcagcaga
     2941 agcgcacgcg cctgttcgag gtcggcgtgt gtttcgccga tgtgaacggc gcggtgattg
     3001 aaaccccgcg catcgccggg cttgccgcgg gcgaggcggt cagcgagcaa tggggcatgc
     3061 ccacgcgcgc gacggatttc cacgacatca agggtgacgc cgcggccttg ttgccggcag
     3121 attgcgagtt cgtggcagcg gcgcatccgg cgttgcatcc gggccgcagt gcgcgcgtgc
     3181 tgcgcaacgg tgcgcatgcc ggctggattg gcgcgctgca tccggccgtt gccgcgaaac
     3241 tcgacctgcc cgaatcgcca ttcttgttcg aactggactg gacggcgatc cgcgaagtcg
     3301 cgttaccaca cgcaaacacg ccgtcggagt ttccgtcgtc gcggcgcgat ctcgccgtgg
     3361 tggtccccga ggtggtgacg gcgcagcaac tgagcacgtt agcgcggcgc gttggtggca
     3421 attcattgca gaaagttatc gtgttcgata tttaccgtgg aaaagcctta ggtgatggat
     3481 tcaaaagcgt ggcttttggc ttgattttca atgactattc gcgcaccctt aacgtagaag
     3541 aaatcgatgc ggcggtggcc gcgatcacgc aggcgttgtc caccgaactg caggcggcga
     3601 tcaggcaata aaggggtgca gcgtggcgtt gaccaaggcc gaactggcgg aggcgctctt
     3661 cgaggaactg ggcctgaaca agcgggaggc caaggagttc gtcgatcttt ttttcgagct
     3721 gatccgcggg cggcttgagc agggcgaaga agtgaagctg tcggggttcg gcaatttcga
     3781 gttgcgttcc aagaaccagc gcccgggccg caatccgaag accggcgaag agattcccat
     3841 ctccgcacgc cgcgtagtca ctttccgccc cggccagaag ctgcgcctga gagtcgagac
     3901 cgatgcggcc ggctgaacag aaatcgctgc cgctgccgga aattccgcgc aagcgctact
     3961 tcgccatcgg cgaggtgagc gaactgtgcg acgtcaagcc gcacgtgctg cgctactggg
     4021 agcaggaatt cccgcagctc aagccggtga agcggcgcgg caaccgccgc tattaccagc
     4081 acgacgacgt gctgatggtg cgccgcatcc gcagcctgct gtacgaccag ggcttcacca
     4141 tcggcggcgc acgccagcgc ctgaaggaaa cgccgaagca gatcccgctg gctgcgggca
     4201 atggatccgt gcaagccaag ccgacgcgcg gtggcgtgcg ccgcgcggtg gaaatccgca
     4261 cgctgcgcaa cgagctcgag tcgctgctca agctgtttcc tcagtaggcg gcttttcatc
     4321 agcagggcgc atccgttacc atgcgcgccc tttccgtggt catgttcggg gcgtagcgca
     4381 gcctggtagc gcacttcctt ggggtggaag tggtcggagg ttcaaatcct ctcgccccga
     4441 ccaactaatt caactgccct aacattgagt tacggcagct tccaccgacc ctgccgcgtc
     4501 gagcgttgct tcgtcgagag ccccgatcag cgtccgcgct gcatccactt gctgccgcag
     4561 gcgcagcacc ggcagacgaa cgcgcatctg gaaatccacc ctggccgcca cctcgccggg
     4621 ggccatggcg gctacttcct cggcggccag cgaattgtcg agctgcgcgg cgcatgccag
     4681 cgcgcttaca tagccggcga gatgcgcagg tttgagtttt agaaagatgc gccagtgctc
     4741 gagcgacgtg cgccagtggc cgcggcggcc agccagattc gcctgcagct ggtgtgcacg
     4801 cgcgtagcgc gggaaacgct gcagcgcctg cgcgagcagt tcctgcgccg cgtccagctg
     4861 gctgtcctgc aacagcctgg cggcctggtt gaccaggctt gcgcgcggat cttccgctgc
     4921 ctccggcgac gagccgtggc cctcggtctg gttcaaatcg cccccgggcc cgtacggccg
     4981 atgtctcaga agttgtatcc gaagtacagg ttgccagtgt aaccctgcgt gcgttccacg
     5041 atcgggctgt cgcgcgccgg tccgaggatc cactcggcgg cgaacagcgt attgagcatc
     5101 cagtgctcgg tcatgaacca gacggcggtg gcgccgaagc gcgcatcctt caggccgccg
     5161 cccggatcat agacccggta acccgagcgc gccgactgcg cagcgttgac cccgaagtaa
     5221 tgatgcatgt aggtgctgtc ggcaaaagtg gccgagggcc cgacgaacac gaagaacttc
     5281 ttgttgccgg cgatcggcga gtacaacgag atgtcgccga tccagccgtt gtgcccgccc
     5341 caggcatggc gcccgttgag gcggatcacc gccgggaacg atacgtactc gtagaacagc
     5401 ttgccttcgg gcgcggcctt gatgctgccc aggcccgaca gtgcaggatc cttctggtcg
     5461 ttgcggccca ggtcatagct caccgcgaag cccgcgcggt aataacgttc cgacaggatg
     5521 ttgtagccca gcccttcgcc gagcgagaag aaccagtcgt cgcggtggcg gatctcgatc
     5581 atcggccccg gctggtagcg gtagcggttg gagccctcga agcgcggcag gtagctggtg
     5641 ccgccgccga gttgcacttc ccacttgggc ggctcgcgca cgaagcgcgg ttccagcgcg
     5701 atgcccgagg agtattgcca ttccgccatg ggcgagtagg tctgcgcatg cgccggccag
     5761 cacaggcaca gcagcagcaa ggcgaggcag cggcgcacaa ggatcagtgg agccatggtt
     5821 cttgttgttg ctctgatatg cggaatgcaa acctagccag tgccagggtg tatctgcaag
     5881 ggcggcccgc cgctttccag ccgctggatc acgccgtcca gcccatacag cgcgatttcc
     5941 accgcgaact gcgcgcggaa gctggccgcc agtgagatgc cattgaccgt gacgtcgtac
     6001 accttccagt cgtcgttgac gcgatgggcg gcgaagccga cctgcagcgg cgcggcctgg
     6061 ggcgatgaaa gatccatgtg cacggtgacg tcgcccgcgc ccggcgccgc ctgcagcgga
     6121 tgccattgct cctgcaggcc atcgccgtaa tcgagcagcg cgtgggcgta ctcatgcacc
     6181 aggctgtttt tgaatgcagc caggaaccgc gagcgctgcg cgtcgctcgc gctcttccac
     6241 tcgcgcccga gcaccaggcg gccgatgtag gggatatcga atcgcggcac caccacctgg
     6301 tcgaccatgc ggtcgaaccg cgcgcggtcg cggcggaatt catcgcggtg ctggtcgatc
     6361 tgcgcaagaa actgcgtgtt ggtgtcgcgg atcgcctgat cgggcgcggc gatggcgagc
     6421 gcgaccacgc acggcgcgaa cagcaggaga gcgaacgtca ggcgcatgac cggtcgtaaa
     6481 gggaggcgga aattcgacct gagcgacgca tggcaggttc gcgcgagccg caaaacgatg
     6541 gaccgcaccg cccctgatga tgttattaat tatagtatta ataaataaca agtatataaa
     6601 taataacatc catcaggccg gtgcggtgga ggcatttccg gcctgtgcga tgcccttggt
     6661 gagcgcaacg gccgaacacc ggacgccggc gcggcgtcca atctgcgact tcgtcagtga
     6721 accccatgga caagagcgcc agcgagtttg ccgaaaccat gccggtcgca
//
LOCUS       DASZZC010000139         7046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_653097,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000139 DASZZC010000000
VERSION     DASZZC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653097"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..884
                     /locus_tag="VHE37_03265"
     CDS             123..884
                     /locus_tag="VHE37_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34577.1"
                     /translation="MLLKHMLIVAMLAIADTAHARDAIELAYATPEGLPFERGDAAGA
                     GSYYALSCEAQQCALVPTELARERGGVLSFDGSETGYTLRAPGEHTLALVRGIAGIKA
                     GPVKTWYASPWFASEAQRYLLHHRHRLYKTVPLDQGTLELRGQWLQQVSACSWCAIGD
                     GLTWKIEYAGLTRTLATIPEDAAAGRTQMPGADDLLLWVGDLDGDGKPDLLLRPQARP
                     DYIELRLYLSTQLVSGKPWTHAARFYWRDPASPST"
     gene            1075..1617
                     /locus_tag="VHE37_03270"
     CDS             1075..1617
                     /locus_tag="VHE37_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HVT34578.1"
                     /translation="MALTATIHHCTVQLADIDRGAYADFELRVARHPSETAEFMLARV
                     LAYCLEFREGLTLTDGVGAVDEPALLVRDLTGRITAWIEVGLPDAARLHRGSKLAGQA
                     AVYTHRDPAQWLAQLAGEKIHRAAEIPVYAFEREFISPAAAALQRRSTLSLSRTEGQL
                     YLDIDGASFSGSLVKRALPA"
     gene            1727..4123
                     /locus_tag="VHE37_03275"
     CDS             1727..4123
                     /locus_tag="VHE37_03275"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HVT34579.1"
                     /translation="MWQPTFRVLAASMLAGVLLAACNTSAGPQTADNGGSGGSTGTST
                     GASTGSTTGGATTGDTTGGTPPPDPYAVKFEVSADRIVAHNGLVERVWSRAPFLTQSL
                     TDLRNHRAWSQASADFSIALATISFSSDALQVSGDPVVADLPNGGRRLTLTLGPTLAA
                     LFPPGFSITRTVELYPGVAGMRSSTQVTSTLPVAMSGYTLDQAVPGNNITATLHAFHA
                     GSDWRDPEWTGPQLVIGDAQPGDWRTDVSGATASGTAQWMSLSDASDARLYYVMERND
                     YASSEMSYDGNTAAARVDLSRDVIYLGPLEEQLHVGNPGSGPGRERLLLPGTPLQLEP
                     VYTGVALGADDEALQHYRYLAGYRMPPYPRAITFNSNGVDANAISTGAKDDLNFTAFQ
                     QQLPIAAQIGIETFVFDDGWQGRSGDWCPDSVSDDPACADPRRGTDAKFDPRFPDAEF
                     KAVQALLISKQMKLGLWMSPLHFNPQAVAFKNHPEWACLPIDAALLAEQENGPYDGSN
                     EAGILQWNAAGVSPDGSRFIDFLEGRIRTAIDDWGVRYFKFDFTAWLDCAGPGTTDLY
                     GYRETFMAMLDRIHADHPDVTIQMDETNDYRLWPFEAIARGPTWYQNGSPSTAEALHA
                     NWVLAPYVPLYALGRNALNAGTLKDYSAGYQMAVALLSHVTFFNDLKSIPADAIPVVK
                     QWTDWYKAHRADIAALAYPLTPDDPLAGDQWAAFQTLDPVTGNGVLLAYRQDAEADTQ
                     TVKLRNIADGNYRLHLAPDEATTQDYSAQQLRDGIALSIGAKRQAQVWRIEQLQGAAR
                     "
     gene            4120..4518
                     /locus_tag="VHE37_03280"
     CDS             4120..4518
                     /locus_tag="VHE37_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34580.1"
                     /translation="MKNAGTLLRCFLAAAFAPLVIPFTGALMFFALHQWLRPSVWGLP
                     PLQSLIGVSINCTMIGYFFTWIYALPLVFILRGLNRYQLRYLLVASALPALSLPFWQV
                     DWKISIVPVLFAGMSTAYAFWRLTAPGNAR"
     gene            complement(4541..5116)
                     /locus_tag="VHE37_03285"
     CDS             complement(4541..5116)
                     /locus_tag="VHE37_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34581.1"
                     /translation="MTEPTAPEKTAAPAVAPAAAAPAAPAVDRAAALAQLRKNALGGA
                     SWFWWIAALSAINSVMIAMNSDTVMALGLGITEMIDIAFKLYGERHAEASAGMIHLLH
                     GAFVFAPIAIFYAIGRRARAGSTAAIKLGMILYALDAAVPILSGDGIGIGFHGLVLFF
                     LWRAHGNTRLLKAAMAMNDQPAPTAAATQAA"
     gene            5273..5512
                     /locus_tag="VHE37_03290"
     CDS             5273..5512
                     /locus_tag="VHE37_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34582.1"
                     /translation="MKTCLACLPVLLLAACANDLAAIRNSTVKAIGGGVDPNAVVLTD
                     VDSSSQSLRWTATTQRGLYKCEGDSGLQQVNCYHP"
     gene            complement(5637..6110)
                     /locus_tag="VHE37_03295"
     CDS             complement(5637..6110)
                     /locus_tag="VHE37_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34583.1"
                     /translation="MHSTIPPARKPMSGGYMPSKANLHRYAGMAALVVDDDEFLRKAL
                     TRQLALLGIGRVAEAGGGERACRALMAQGPFDLVTCDLSMPGLDGMKLLAELAAIQPS
                     IGVLLVTGMPRESIEAAEQQARALRLNLLGSLRKPVQLRELDQLMQRSHVAPAAG"
     gene            complement(6128..7006)
                     /locus_tag="VHE37_03300"
     CDS             complement(6128..7006)
                     /locus_tag="VHE37_03300"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVT34584.1"
                     /translation="MSAVLRELPAAPAASPSGERLLQLIEQQVRAQQLALPMLPEVAQ
                     RIGGMARRDDIQFAVIAAVIAKDPACSAQIMRVANSAAMQGNGGAVSSLQLAVTRLGL
                     DLTRALVMRMAMDRMFRARSPLLNKILMTTWTRSLWVAALSRVLAARYTRVNPDAAML
                     AGLLHLVGSLPMIQLLDREPQLVDSGADMLRSILTLHWRVAHLVLEAWNFPPELRELP
                     AQAFDFSRRHDGPADAADVVSLAILQTYGDDYRHWVAVDPQQVDAFRQLGLQPGSPQL
                     QETHAAFKNSMQLLNG"
BASE COUNT         1143 a         2471 c         2321 g         1111 t
ORIGIN      
        1 agtccgcggc aagtgtagcc agatcgcggc ggagcggact gcgacgatgt tccacatctc
       61 ctgcaccggg cttgcacagg cgctgcgcag actgggttca cgcccaccgc ggagtccgtc
      121 ccatgttgct caagcacatg ctgatcgtcg ccatgcttgc aatcgctgac accgcacatg
      181 cgcgcgacgc catcgagctc gcctacgcca cgcccgaagg cctgccgttc gagcgcggcg
      241 acgcggccgg cgcgggcagc tactacgcgc tcagctgcga agcacagcaa tgcgcgctgg
      301 tgcccaccga gctcgcgcgc gagcgcggcg gcgtgctgtc gttcgacggc agcgaaaccg
      361 gctacacgct gcgcgcgccg ggcgagcaca cgctggcgct ggtgcgcggc atcgccggca
      421 tcaaggccgg gccggtgaag acctggtacg ccagcccgtg gttcgccagc gaggcgcagc
      481 gctacctgct gcatcaccgc catcgcttgt acaaaacggt gccgctggac cagggcacgc
      541 tggaactgcg cgggcaatgg ctgcagcagg tgagcgcctg cagctggtgc gcgatcggcg
      601 acgggctgac gtggaagatc gagtacgccg gactcacgcg cacgctcgcc accattcccg
      661 aggacgccgc cgccggccgc acgcagatgc ccggcgccga cgatctgctg ctgtgggtcg
      721 gcgatctcga cggcgacggc aagcccgacc tgctgctgcg gccgcaggcg cggcccgact
      781 acatcgagct gcggctgtac ctgtccacgc aactggtttc cggcaagccg tggacgcacg
      841 cggcgcgctt ctactggcgc gatccggcga gcccgtccac atgaatccgc gagcgcgctg
      901 gcgagaacgg atcgccattt tcggaggtca ccgcaccctg gttttacttg ttatttatta
      961 tttatatatt tttcatacat taaataatta ataacatcgc caggcagggt gcggtggacg
     1021 tgatttgccc cgctgcatcg ccgcgcagcg gcgttgcgtt aatctgcgcg ccccatggca
     1081 ctgaccgcga ccatccatca ctgcaccgtc cagctggccg acatcgatcg cggcgcgtat
     1141 gccgacttcg agctgcgcgt ggcgcggcac ccgtcggaaa ccgcggagtt catgctggca
     1201 cgcgtgctgg cctactgcct ggaattccgc gaagggctga cgctgaccga cggtgtcggg
     1261 gcggtcgacg aaccggcgct gctggtgcgc gatctcaccg ggcgcatcac cgcgtggatc
     1321 gaggtgggcc tgcccgacgc cgcgcgcctg catcgcggca gcaagctcgc gggacaggcg
     1381 gcggtgtaca cgcacaggga tccggcgcaa tggctggcgc aactggccgg cgaaaaaatc
     1441 catcgcgccg cggaaattcc ggtgtacgcg ttcgagcgcg aattcatctc gccggccgcg
     1501 gcggcgctgc agcggcgcag cacgctgtcg ctgtcgcgca ccgagggcca gctgtacctg
     1561 gacatcgacg gtgcatcctt cagcggctca ctggtgaagc gcgcgctgcc ggcctgacgc
     1621 tcccgcggcg cagtgcgttt ttcctacggg gcgcgctgcg acgtgcatgt gaaaatcgtc
     1681 cgcacgatga gtccgcaacg gaccgcgcgg gggagagaga acaaccatgt ggcaaccgac
     1741 attccgcgtg ctcgcggcca gcatgctcgc cggcgtgctg ctggcggcat gcaacaccag
     1801 cgccggtccg cagactgccg acaacggcgg cagcggcggc agcactggca catccaccgg
     1861 cgcgtccacc ggcagtacga ccggcggcgc aacgaccggc gataccaccg gcggcacgcc
     1921 gccgcccgat ccctacgcgg tgaaattcga ggtgagcgcg gaccggatcg tcgcgcacaa
     1981 cggactggtc gagcgcgtgt ggtcgcgcgc gccgttcctc acgcagagcc tcaccgacct
     2041 gcgcaaccac cgcgcgtggt cgcaggccag cgcggatttc tccatcgcgc tggcgacgat
     2101 cagcttctcg tccgacgcgc tgcaggtgag cggcgacccg gtcgtcgccg acctgcccaa
     2161 cggcggcagg cgcctcacgc tcacgctcgg gcccacgctc gctgcgctgt tcccgcccgg
     2221 cttttccatc acgcgcacgg tggagctgta tccgggcgtg gccggcatgc gcagcagcac
     2281 gcaggtcacc agcacgctgc cggtcgcaat gtccggctac acgctggacc aggccgtgcc
     2341 gggcaacaac atcacggcca cgctgcacgc gttccacgcc ggctccgact ggcgcgaccc
     2401 cgaatggacc ggcccgcagc tggtgatcgg cgacgcgcag cccggcgact ggcgcaccga
     2461 cgtttccggc gccaccgcgt cgggaaccgc gcagtggatg tcgctgagcg acgccagcga
     2521 cgcgcgcctc tactacgtga tggagcgcaa cgactacgcc tcttcggaaa tgagttatga
     2581 cggcaacacc gccgccgcgc gcgtggacct gtcgcgcgac gtcatctacc tcggtccgct
     2641 ggaagagcag ctgcatgtcg gcaaccccgg ctccggcccc ggccgcgagc gcctgctgct
     2701 gcccggcacg ccgctgcagc tggagccggt gtacaccggc gttgcgctcg gcgccgacga
     2761 cgaagcgctg cagcactacc gctatctcgc cggctaccgc atgccgccct atccgcgcgc
     2821 catcaccttc aactccaacg gcgtcgatgc caacgccatc tccaccggcg ccaaggacga
     2881 cctgaacttc accgcgttcc agcagcagct gccgatcgcc gcgcagatcg gcatcgagac
     2941 tttcgtgttc gacgacggct ggcagggccg ctccggcgac tggtgcccgg actcggtgtc
     3001 cgacgatccg gcctgcgccg atccgcgccg cggcaccgat gcgaaattcg atccacgctt
     3061 tcccgatgcg gaattcaagg cggtgcaggc gctactgatt tcgaagcaga tgaagctggg
     3121 cctgtggatg agcccgctgc acttcaatcc gcaggcggtg gcgttcaaga atcaccccga
     3181 gtgggcttgc ctgccgatcg acgccgcgct gctggccgag caggagaacg gcccctacga
     3241 cggctccaac gaagccggca tcctgcagtg gaatgccgcc ggcgtgtcgc ccgacggcag
     3301 caggttcatc gacttcctcg aaggccgcat ccgcaccgcc atcgacgact ggggcgtgcg
     3361 ctacttcaag ttcgacttca ccgcctggct cgactgcgcc ggccccggca ccaccgacct
     3421 gtacggctac cgcgaaacct tcatggcgat gctcgaccgc atccacgctg accacccgga
     3481 cgtgaccatc cagatggacg aaaccaacga ctaccgcctg tggccgttcg aagcgattgc
     3541 gcgcggaccc acgtggtacc agaacggctc accctccacc gccgaggcgc tgcacgccaa
     3601 ctgggtgctg gcaccgtacg tgccgctgta cgcgctcggc cgcaacgcgc tcaacgccgg
     3661 cacgctcaag gattattccg ccggctacca gatggcggtg gcgctgctgt cgcacgtcac
     3721 cttcttcaac gacctgaagt cgatccccgc cgacgccatt ccggtggtga agcaatggac
     3781 cgactggtac aaggcgcatc gcgccgacat cgccgcgctg gcgtatccgc tgacgccgga
     3841 cgatccgctg gccggcgacc agtgggccgc gttccagacg ctcgatccgg taaccggcaa
     3901 cggcgtgctg ctcgcgtacc ggcaggatgc ggaagcggac acgcagacgg tgaagctgcg
     3961 caacatcgcc gatggcaatt accggctgca tctcgcgccg gatgaggcaa cgactcagga
     4021 ctacagcgcg cagcagttgc gggatggcat tgcgctcagc atcggcgcga agcgccaggc
     4081 gcaggtgtgg cgcatcgaac aactgcaggg cgccgctcga tgaaaaacgc gggcaccctg
     4141 ctccggtgtt tcctggcggc ggcattcgcc ccgctggtga tccccttcac cggcgcgttg
     4201 atgttcttcg cgctgcacca gtggctgcgc ccttcggtgt ggggcctgcc gccgctgcag
     4261 tcgctgatcg gcgtgtcgat caactgcacc atgatcggct acttcttcac ctggatttac
     4321 gcgctgccgc tggtcttcat cctgcgcgga ctgaaccgct accagctgcg ctacctgctg
     4381 gtggccagcg cgctgccggc gctgagcctg ccgttctggc aggtggattg gaaaatttcc
     4441 atcgtgcccg tcctgttcgc gggcatgtcg acggcgtacg cgttctggcg cctgacggcg
     4501 cccggcaacg cccgctgacg cgccgtccgg cggcgtccgc tcaggccgcc tgcgtcgcgg
     4561 ccgccgtcgg cgccggctga tcgttcatgg ccatcgccgc cttgagcagc cgggtgttgc
     4621 catgcgcacg ccacaggaag aacagcacca gcccgtggaa gccgatgccg atgccgtcgc
     4681 ccgagaggat gggcaccgcg gcatccagcg cgtagaggat catgccgagc ttgatggcag
     4741 cggtgctgcc cgcgcgggca cgtcggccta tggcgtagaa gatggcgatg ggcgcgaaca
     4801 cgaacgcgcc atgcagcagg tgaatcatcc ccgcgctggc ctccgcatgc cgttcgccgt
     4861 acagcttgaa ggcgatgtcg atcatctcgg tgatgcccag gcccagcgcc atcaccgtgt
     4921 cggaattcat ggcgatcatc acgctgttga tggccgacag cgcggcgatc caccagaacc
     4981 agctggctcc gcccaacgcg ttcttccgca gctgcgcgag cgcagcggcg cgatcgaccg
     5041 ccggcgcagc gggcgcggca gccgcgggtg caacggcggg cgcagccgtt ttttccggcg
     5101 cagtgggctc tgtcatgtgt tctcccttcg atggccgttg cattcccccg aatgcaactg
     5161 gtgcccgcag actagcacca cgaacggcgc cgctctagcg gccgcccacc gtcagcccgc
     5221 tgcaacaggg ccgcgctaga gttgcgtcaa catctttgcg gggagttttt ccatgaagac
     5281 atgccttgcc tgcctgcccg tactgctgct cgccgcctgc gccaacgatc tggccgcgat
     5341 caggaacagc acggtgaagg cgatcggcgg cggtgttgat cccaacgccg tggtgctcac
     5401 cgacgtggac agcagctcgc agtcgctgcg ctggaccgcg accacccagc gcgggctgta
     5461 caagtgcgaa ggcgacagcg gactgcagca ggtgaactgc taccacccct gaaaaaggcc
     5521 tgaccgcact gctcgcgcgg gtgttattaa ttatagtacg ttttaatttt taatatattt
     5581 tttataacac gctggagggc ggtgcggtgg cagcaaaaat cgccacgccg cgcacctcag
     5641 ccggccgcag gcgccacgtg gctgcgctgc atgagctgat cgagctcgcg cagctgcacc
     5701 ggtttgcgca gcgagcccag caggttgagc cggagcgcgc gcgcctgctg ttcggcggcc
     5761 tcgatgcttt cgcgcggcat gccggtgacc agcagcacgc cgatgctcgg ctggatggcg
     5821 gcaagctcgg ccagcagctt catgccgtcg agcccgggca tggaaagatc gcaggtgacc
     5881 aggtcgaacg ggccctgcgc catcagcgcg cggcaggcgc gctcgccgcc gccggcctcg
     5941 gccacgcggc cgatgcccag cagcgccagc tggcgcgtga gcgccttgcg caggaattcg
     6001 tcgtcgtcga ccaccagcgc cgccatgccg gcgtagcggt gcaggttggc cttcgacggc
     6061 atgtaaccgc ccgacatcgg cttgcgcgca ggcgggatgg tgctgtgcat ggtgcgctcc
     6121 atatgcctca gccgttgagc agctgcatgc tgtttttgaa tgccgcgtgc gtttcctgca
     6181 gctgcggcga gcccggctgc agacccagtt gccggaaggc gtcgacctgc tgcggatcga
     6241 ccgcgaccca gtgccggtaa tcgtcgccat aggtctgcag gatggccagg ctgaccacgt
     6301 cggcggcatc ggccggcccg tcatggcgcc gcgagaaatc gaacgcctgc gccggcagct
     6361 cgcgcagttc cggcgggaag ttccacgcct ccagcaccag gtgcgcgacg cgccagtgca
     6421 gcgtgaggat gctgcgcagc atgtccgcgc cgctgtcgac cagctgcggc tcccggtcga
     6481 gcagctggat catcggcagc gagcccacca ggtgcagcag gccggcaagc atggcggcat
     6541 ccgggttcac gcgcgtgtag cgcgcggcca gcacgcggct gagcgcggcc acccacaggc
     6601 tgcgcgtcca ggtggtcatc aggatcttgt tcagcagcgg cgagcgcgcg cggaacatgc
     6661 ggtccatggc catgcgcatg accagcgcgc gggtgaggtc caggcccagg cgcgtcaccg
     6721 ccagctgcag cgagctcacc gcgccgccgt tgccctgcat ggccgcgctg ttggccacgc
     6781 gcatgatctg cgcgctgcag gccgggtcct tcgcgatgac ggcggcgatc acggcaaatt
     6841 ggatgtcgtc gcgccgcgcc atgccgccga tgcgctgggc cacctccggc agcatgggca
     6901 gcgccagctg ctgggcgcgc acctgttgct cgatcagctg cagcagtcgc tcgcccgacg
     6961 ggctggccgc cggcgcggcc ggcagttcac gcaatacggc gctcatgcgc gtctctccat
     7021 cggaaaacgc acgcagtctg cgccgg
//
LOCUS       DASZZC010000140         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_973872,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000140 DASZZC010000000
VERSION     DASZZC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973872"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VHE37_03305"
     CDS             complement(<1..1626)
                     /locus_tag="VHE37_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVT34585.1"
                     /translation="MSKTDRPGARAGSDADAGDAPAAAKLIWRFEGYVLDERGPSLSR
                     DGKALECQVKPLAVLIHLLEHAGEVVTKDELLEHCWPGRIPSESVLTTAMTKLREVLD
                     DADQRLVKTVYGYGYRFAASVQRAAVDGADPLARDFRIGDAPPQRPLWKLAEKLGAGG
                     ETWLARHAKTGEARVFKFAPDAAALRQIKREITLFRLLEEHPAARGSYRRLLDWNLVE
                     PPFFIEAEYLAGGSLQQWIERCGGWSAVTLTQRLELAVQIACALDVAHQAGVLHKDLK
                     PANILIDDAVTPAQVRLADFGSGGLLDRERLAELGFTQLGFTQTVGALSERTRGTLLY
                     AAPEVLGGAMPTLQSDIYSLGVILYQIIVGDWSAILAPGWERGISDELLREDIAACTD
                     QSPPRRLAGAALLAQRLQTLEARRAQRVALAAEREESERLRRRVAQARARRPWVAALL
                     GAMVIGIAASSLFAWRAEQSRKAANHHAAMQEAASEFLSRILGAGDPLLGVTGPDNMA
                     ARAVVDRAAAEAAGSFADQPEQEAVVRLMIGNVY"
     gene            2108..5692
                     /locus_tag="VHE37_03310"
     CDS             2108..5692
                     /locus_tag="VHE37_03310"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HVT34586.1"
                     /translation="MKTLKVARVPRATAASIHVRNAASETAAGEFGTLCRLIAARAVP
                     VATLAALGLAFAPVAQARTIEVAGGDVTANDVAHCSLLDAFAAANTNTAKGACPAGDD
                     ADSGGDIIHISDINSTFALSVSSLHVTGKVTVQGNGSTFNGDIGSPVLIVDEGGNLTL
                     GNATVANNFSTSNGAGLANYGTLTLNDVTVTGNRAGGQGGGIFNTGALTLNRSNVSNN
                     KSSQDGAGIGSTGNSSVTLNDSTVAGNSTQYCRGDGIGAFYKTVLVLNRTTVSGNTGA
                     QKGSGIYVSGSSTLSLNQSTVSDNETASSPFSHSYYGGGIFTLGTTTITSSTLSGNVS
                     HGDAGAIYSGAVVTLNNSTVSDNSGPAIELAGGSLTLKNSTVAGNRSDGITLIGARAN
                     IDNSIIAGNGGVNCSRVDGNANSMADDDSCGSTTKVSPDDLKLQPLANNGGPTKTMAL
                     GEGSVAIGAGDASICSSAPIDSLDQRGYARPADSCDIGAFDSGGTPPFVPDTTPDSFS
                     FTAATGATRGASVTSAPATITGINTDASASITGGTFSINGGEPVTSGTVKNNDSITIT
                     QTASSSFSTKTTAILTIGGVDGSFDVTTEAADTTPATFSFDDVDGAALSATGVTSNSV
                     TISGINTSASVSVTGDSGALVSINGGSFASSGSVTSGQTLQAEVNASATCGTQTTASV
                     TVGTVTVPFHVNTAACDSTPNSFSFAASSDADPGSSQTSAAAVITGINTDVEASISGG
                     TFSINGGQPLTSGTVRNNDSVKVTLSASSSFGGDASATLTIGGVQGTYTVTTREGHTN
                     PTPFSFDPVTGATPGASVVSGSALVSGLEVAAPISIDNGQFCIGSTSNCFNNSLMTVN
                     NNELVYVRLNASNSFSTSTTSTLTIGGVSGSFSVTTAAQDSTPDAIGFAGRTVAAGQS
                     YETALKAVTGINTPITASLDASSDTSAQLLVNGHAVTGASATVSLNDTIGLRLTPAAA
                     GGSVVTARLTLGTVHTSWSLTTRIDDLPVAFHFSDRKDVAGGQTFTTDAKAVSGVNVQ
                     VAAVVSGDNSAKLLVNGNPAPSGTMVGAGDTVALQLTSSKAGGTTVSASLTLGSAAPA
                     GWNITTRRDDTPDAFSFADTAARAGSTFTTGGKLIRYINVDVPATVNSDGRAVLLIDG
                     VESASGTLVHAGQKVALRMTAAANKGSVVHATLTVGGYTAPQWTLTSK"
BASE COUNT         1207 a         2088 c         1918 g          986 t
ORIGIN      
        1 gtaaacgttg ccgatcatca agcggaccac ggcctcctgt tcgggttgat cggcgaagct
       61 gcccgcggct tctgcagccg cgcggtcgac caccgcgcgc gcggccatgt tgtcaggacc
      121 ggttacgccc agcagcgggt cgccggcgcc caggatgcga gagagaaatt cgctggcggc
      181 ctcttgcatc gccgcgtggt ggttcgccgc tttgcgcgat tgttccgcgc gccaggcaaa
      241 caggctgctg gcggcgatgc cgatcaccat ggcaccgagc aacgcagcca cccacggtct
      301 gcgcgcgcgc gcctgcgcca cacgcctgcg caagcgctcg ctctcttcac gttcggctgc
      361 cagtgccacg cgctgggcac gccgcgcctc gagggtttgc agccgctggg ccagcaacgc
      421 ggcgccggcc aggcggcgcg gaggcgactg gtcggtgcag gcggcaatgt cctcgcgcaa
      481 cagttcgtcg cttatgccgc gttcccagcc cggcgccagg atcgcactcc agtcgccgac
      541 gatgatctgg tacaggatca cgcccagcga gtagatgtcg gactgcaggg tcggcatggc
      601 gccgcccagc acttcgggtg ctgcgtagag cagggtgccg cgcgtgcgct ccgacagggc
      661 tccgacggtc tgggtgaagc ccagttgcgt gaaccccagt tcggccaggc gctcgcggtc
      721 cagcaagccg ccgctgccga aatccgccag ccgcacctgc gccggcgtca ccgcatcgtc
      781 gatcaggata ttggccggct tcaggtcctt gtgcagcacg cccgcctggt gcgcaacgtc
      841 gagcgcgcac gcgatctgca ccgcaagctc cagccgctgt gtcagcgtga ccgcgctcca
      901 gccgccgcag cgctcgatcc actgctgcag gcttccgccc gccaggtact cggcttcaat
      961 gaaaaacggc ggctcgacca gattccagtc aagcaggcgc cggtagctgc cgcgcgcggc
     1021 cgggtgttct tccagcaggc gaaacaaggt gatctcgcgc ttgatctggc gcagcgccgc
     1081 ggcatcgggc gcaaacttga acacgcgcgc ctcgccggtt ttggcatgcc gggccagcca
     1141 ggtttcgccg ccggcaccca gtttctcggc cagcttccac aagggtcgct gcggcggcgc
     1201 atcgccgatg cggaagtcgc gcgccagcgg atcggcgcca tccaccgcgg cgcgctgcac
     1261 gctggccgcg aagcggtagc cgtagccgta gaccgtcttg accaggcgct gatcggcatc
     1321 gtccagcact tcgcgcagct tggtcatggc cgtggtcagc accgactcgc tgggtatgcg
     1381 gccgggccag caatgctcca gcaactcgtc cttggtgacg acttcgccgg cgtgctcgag
     1441 caggtggatc agcacggcga gcggcttgac ctggcattcc agcgccttgc cgtcccgcga
     1501 caaagacggg ccgcgctcat ccagcacata gccttcaaag cgccagatca gcttggccgc
     1561 tgcgggcgca tctcccgcgt ccgcgtcgct gcctgcccgc gcccccggac gatcggtctt
     1621 gctcatgctt cccccgcggg attgaaaggc ccgctgcgcc aatacgctac cgcgccaccc
     1681 gcaagcgcga caacgtgcgt ttgcggcatc cgggacgcgg ggtgacgtcc gaccgcatgc
     1741 cattctcgtc cgttattaaa tatagtataa atataaaaat atacgatata ataaaacatc
     1801 aataccagcg gggcggtcag gcccagatgg agccttcgca ccagttagta attgttggga
     1861 agaagttcag gattgcccaa gggcgaaacg ctacggtgcc attggtgttg tgcagggaga
     1921 gaattgtgga tcaaacatac cagtcgttga cgcaaacgca gccaacggta aagattacaa
     1981 acatggggac tggcggcgct cgctgcctcg tcgagagtga ccaatcgata caccaccgca
     2041 gcatcaggct gcgacgaggt accccgtgcc agggaagcac gggcaagcaa caaccggggg
     2101 aatgcctatg aagaccttga aggtcgcgcg cgtgccgcgc gcaactgctg cgtccatcca
     2161 tgtccgtaac gccgccagtg aaactgctgc tggtgaattc ggcacgctgt gccggctgat
     2221 cgccgcgcgc gcggtgcccg tcgccacgct ggcggcgctg ggcttggctt tcgcgccggt
     2281 ggcgcaggca cgtaccatcg aagtggccgg tggggatgtc accgccaacg atgtcgcgca
     2341 ttgctcgctg ctcgacgcat ttgccgccgc caacaccaac accgccaagg gcgcctgccc
     2401 ggccggcgac gacgccgaca gcggcggcga catcattcac atcagcgata tcaacagcac
     2461 gtttgccctg tccgtttctt ccttgcatgt caccggcaag gtcaccgttc agggaaatgg
     2521 cagcacgttc aatggcgaca tcggatcgcc cgttctgatc gtcgacgagg gcggcaacct
     2581 gacgctgggc aacgcaacgg tggcaaacaa cttttcgacc agcaacggcg caggactggc
     2641 gaattacggc acgctcacct tgaatgacgt cacggtcacg gggaaccgag ccggcggcca
     2701 gggcgggggc atctttaaca cgggcgcact gactctgaac cgcagcaacg tatccaacaa
     2761 caaatcctca caggatggcg ccggaattgg gagcaccggc aattcttcgg tgacgctcaa
     2821 cgattccacg gttgccggga acagtaccca gtactgcagg ggcgacggca tcggcgcttt
     2881 ttacaagacc gtcctggtct tgaaccgcac cacggtgagc ggtaatacgg gtgcccagaa
     2941 gggcagtggc atctacgtga gcggttcaag cacgctcagc ctgaaccaga gcacggtgtc
     3001 cgacaacgag actgcctcaa gccctttctc ccactcttac tacgggggcg gcatcttcac
     3061 cctgggaacc acgacgatca ccagcagtac gctgtccggc aatgtgtctc atggtgacgc
     3121 cggcgcaatc tatagcggag cggtagttac cctgaataac agcaccgtgt ccgacaacag
     3181 cggtcccgcg attgaactgg caggcggttc gctgacgttg aagaacagca cggttgcggg
     3241 caatcgcagc gatggaatca cactaattgg ggctcgagcc aatatcgaca actcgatcat
     3301 tgccggcaac ggtggcgtca actgcagcag agtcgatggc aacgccaaca gcatggccga
     3361 cgatgactca tgcggcagca ccacgaaggt ttcgccggac gatctcaagc tgcagcccct
     3421 ggccaacaat ggtggcccga ccaaaaccat ggcgctggga gagggcagtg tcgccatcgg
     3481 cgccggtgac gcgtcgatct gctcgtcggc gccgatcgac agtctcgacc agcgcggcta
     3541 cgcgcgcccg gcggactcct gcgacatcgg cgcgtttgat tccggtggta cgccgccatt
     3601 cgtgcccgac accacccccg acagcttcag cttcacggcg gcgaccggcg ccaccagggg
     3661 cgcgagcgtc acttccgcac cggcgaccat caccggcatc aacaccgacg ccagcgccag
     3721 catcaccggc ggcaccttca gcatcaacgg tggagaaccg gtcaccagcg gcaccgtcaa
     3781 aaacaacgac agcatcacca tcacccagac cgcgtcatcg agtttctcca ccaagaccac
     3841 cgccatcctg accatcggcg gcgtagacgg ctccttcgac gtcaccaccg aggccgcgga
     3901 caccacgccc gccaccttca gcttcgacga tgtcgacggc gcagccctct ccgccaccgg
     3961 cgtcaccagc aacagcgtca ccatcagcgg catcaacacc agcgcatcgg tgtcagtgac
     4021 cggcgacagc ggcgcgctgg tcagcatcaa cggcggctcg tttgccagtt ccggcagcgt
     4081 caccagcggc cagaccctgc aggccgaggt caatgcctcc gccacctgcg gcacgcagac
     4141 cacggcaagc gtcaccgtcg gcaccgtcac cgttccgttc cacgtcaaca cggcggcatg
     4201 cgacagcacg ccaaacagct tcagctttgc ggccagcagc gacgcggacc cgggcagttc
     4261 gcagacttct gccgcggccg tcatcaccgg catcaacacc gacgtcgagg ccagcatcag
     4321 cggcggcacc ttcagcatca acggtggaca accgctcacc agcggcaccg tgcgcaacaa
     4381 cgacagcgtc aaggtgacgc tgagcgcatc gtcgtcattt ggcggcgacg cttcggccac
     4441 gctgaccatc ggcggcgtgc agggcaccta caccgtcacc acgcgcgaag gccacaccaa
     4501 tcccacgccg ttctcgtttg atccggtgac cggcgccact ccgggcgcca gcgtggtgtc
     4561 cggttccgcg ctggtcagcg ggctggaagt ggccgcgcct atcagcatcg acaacggcca
     4621 gttctgcatc ggcagcacca gcaactgttt caataacagt ctgatgacgg tgaacaacaa
     4681 cgagctggtc tacgtgcggc tgaatgcatc caacagcttc agcaccagca ccacttcgac
     4741 gctgaccatt ggcggggtca gcggcagctt cagcgtgacc acggcggcac aggacagcac
     4801 gcccgatgcg atcggctttg ccggcaggac ggtcgccgcg ggccagtcct acgaaaccgc
     4861 gctaaaggca gtgacgggca tcaacacccc catcacggcc tcgcttgatg cgagcagcga
     4921 caccagcgcg caactgcttg tcaacggcca cgcggtgacc ggcgccagcg caaccgtgag
     4981 cctcaatgac acaatcggcc tgcgcctgac tccggctgcc gccggcggca gcgtggtcac
     5041 ggcgcggctg accctgggca ctgtgcatac cagctggagc ctaaccacga ggatcgatga
     5101 cctgccggtg gctttccact tcagcgacag gaaggacgtg gcgggcggcc agaccttcac
     5161 caccgatgcc aaggcggtca gcggcgtcaa cgtgcaggtt gctgccgtgg tcagcggcga
     5221 caacagtgca aagctgctgg tcaacggcaa tccggcaccc agcggcacca tggtcggtgc
     5281 aggggatacg gtggcgctgc aactcacctc gagcaaggcc ggtggcacca cggtcagcgc
     5341 aagccttacc ctgggcagcg cggcgcccgc gggttggaat atcaccaccc ggcgggatga
     5401 cacacctgac gccttcagct tcgccgacac agcggcgcgg gcgggcagta ctttcaccac
     5461 cggcggcaaa ctgatccggt atatcaacgt cgacgtgccg gcaacggtca acagcgatgg
     5521 ccgggcggta ctgctgatcg atggcgtgga atccgccagc ggcacgctgg tccatgcggg
     5581 ccagaaggtt gcgctgcgca tgaccgcagc agcgaacaag ggcagcgtgg tgcatgcaac
     5641 gctcaccgtc ggcggctaca ccgcgccgca gtggacattg acctcgaagt gaggcgctgc
     5701 agggcggtgt gcggacgcga aaaccgcaca ccgcccccgt ttttctgaag ttattaatta
     5761 ttgtccgaat gtaaaataaa acaatagtta ataacagcga aaatgcggat gcggtgacat
     5821 gcggctcgtg gcttcacgac gcgtgcagac gaaacaaggt gcgcacaact gcctcggtgg
     5881 agcgacgttc gctggacgtg tgatctggat tagccctctc ttcgtccgac accattgcgc
     5941 gatcacgcga agcgttcgtg caaaacgatg tgactccggc gccccgcagc gcacgggcaa
     6001 tggcgtgcgc ggctgagcct gctcaggcgc gcagtgcgcg aacctggcgc tcgaactgct
     6061 cctgcagctg tgccgatggt tcttccaggt agcgcaggcg caggtacgtt tccagcagcg
     6121 gtgccaacgt ttcggcgagc tccggaaatt cgcgggatgc gcgcaggatg aaatcccgcg
     6181 gaccctcgcc gggctgctg
//
LOCUS       DASZZC010000141         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_462503,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000141 DASZZC010000000
VERSION     DASZZC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462503"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VHE37_03315"
     CDS             <1..795
                     /locus_tag="VHE37_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HVT34587.1"
                     /translation="VLFGIVLLLIVGLLIRLNPFVSVSAGHKGVVTLFGKVDPAGLDE
                     GLHVVNPFASVTDVSVRIQKADAEGDAASRDLQQVTTHITLNYHINPSTVPAFWQAIG
                     PGYVDTIITPEVQETFKAVTARYTAEELITKREEVRAQIRDTLNQKVNELTHGGLVMD
                     EFSITNFAFSHSFNAAIEAKQEAEQLALKAKRDLDRIQVEAQQKIAMAEAEAASLKAQ
                     KQEITPDLIRLREIEVQREAILKWDGHLPNVNSGAVPFINIGSQAH"
     gene            975..1958
                     /locus_tag="VHE37_03320"
     CDS             975..1958
                     /locus_tag="VHE37_03320"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HVT34588.1"
                     /translation="MKKPVSVTITGAGGQIAYSLIFRVASGSMLGPDQPVILKLLEVP
                     VPEVLEKLKGTVMEIDDCAFPLVAGVEVHSDPMSGFEGANYALLVGARPRGPGMERKD
                     LLTANGGIFGPQGKAIAAKANKDIKVLVVGNPANTNALIAATAAKGLDPKQFHAMVRL
                     DHNRAVSQLAAKTSAHNTDVEKMIIWGNHSSTQYPDISHATVKGKPAKSLVDQNWIEK
                     DFIPVVQQRGAAVIKARGLSSAASAASAAVDHMRDWALGSNGKWVSMGVPSDGSYGIK
                     PGIVYGYPCICKNGSYEIVQGLSVDEFSRARMDATEKELREERAAIEDLLK"
BASE COUNT          420 a          694 c          698 g          345 t
ORIGIN      
        1 gtattgttcg gcatcgtgct gctgctgatc gtgggactgc tgatccggct caacccgttc
       61 gtctcggtgt cggccggcca caagggcgtg gtcacgctgt tcggcaaggt cgacccggcc
      121 gggctcgacg agggactgca cgtggtcaac ccgttcgcct cggtcaccga cgtgtcggtg
      181 cgcatccaga aggccgatgc cgagggcgac gccgccagcc gcgacctgca gcaggtgacc
      241 acccacatca cgctcaacta ccacatcaat ccgtccacgg tgccggcgtt ctggcaggcg
      301 atcgggcccg gctacgtcga caccatcatc acgccggaag tgcaggagac gttcaaggcc
      361 gtcaccgcgc gctacaccgc ggaagagctg atcaccaagc gcgaggaagt gcgcgcgcag
      421 atccgcgaca cgctcaacca gaaggtcaac gaactgactc acggcggcct ggtgatggac
      481 gagttctcca tcaccaactt tgccttctcg cattcgttca acgcggccat cgaggccaag
      541 caggaagccg agcagctggc gctgaaggcc aagcgcgacc tcgaccgcat ccaggtggaa
      601 gcacagcaga agatcgccat ggccgaggcg gaagcggcca gcctgaaggc gcagaagcag
      661 gagatcacgc ccgacctgat ccgcctgcgc gagatcgagg tgcagcgcga ggcgatcctc
      721 aagtgggacg gccacctgcc caacgtcaac agcggcgcgg tgccgttcat caacatcggc
      781 agccaggcgc actgagcttt ggggcggctt ttttgcctcc accgcacccc ggtttgcgct
      841 gttattaatt attgtctaat ttaataatta tacgatattt agtaacatac aaaatgcggg
      901 tgcggttgga tgctgtggcg caataaagta tccttcgcgc cctcattttt cctcgcagat
      961 atggagtttt cccgatgaag aagcccgtca gcgtcaccat caccggcgcc ggtggccaga
     1021 tcgcctactc gctgatcttc cgcgtggcct cgggctccat gctcggcccc gaccagccgg
     1081 tgatcctcaa gctgctggaa gtgccggtgc ccgaagtgct ggagaagctc aagggcacgg
     1141 tgatggagat cgacgactgc gccttcccgc tggtggccgg cgtggaagtg cattctgatc
     1201 cgatgtcggg ttttgaaggc gccaactacg cgctgctggt cggcgcgcgc ccgcgcggcc
     1261 ccggcatgga gcgcaaggac ctgctgaccg ccaacggcgg catcttcggg ccgcagggca
     1321 aggcgattgc ggcgaaggcg aacaaggaca tcaaggtgct ggtggtcggc aacccggcca
     1381 acaccaacgc gctgatcgcg gccaccgcgg ccaagggcct cgatccgaag cagttccacg
     1441 ccatggtgcg gctggaccac aaccgcgcag tgtcgcagct cgccgccaag accagcgcgc
     1501 acaacaccga cgtggaaaaa atgatcatct ggggcaacca cagctccacg cagtaccccg
     1561 acatcagcca cgccacggtc aagggcaagc cggcaaagtc gctggtcgac cagaactgga
     1621 tcgagaagga cttcatcccg gtggtgcagc agcgcggcgc ggcggtgatc aaggcgcgcg
     1681 gcctgtcgag cgcggccagc gccgccagcg cggcggtcga ccacatgcgc gactgggcgc
     1741 tgggttccaa cggcaagtgg gtcagcatgg gcgtgcccag cgacggcagc tatggcatca
     1801 agccgggcat cgtttacggc tacccctgca tctgcaagaa cggcagctac gagatcgtgc
     1861 agggcctgtc ggtcgacgag ttctcgcgtg cgcgcatgga tgccaccgag aaggagctgc
     1921 gcgaggagcg cgcggcgatc gaagacctgc tcaagtagtt tcgcaacgcg cgcccgcgcg
     1981 agatggaaat cctgcacctg gtcgaaggcc agccgccgca gcggctggac tcgctgacca
     2041 cgctgccgcc cgccgggctg gtgtggatcg acttcatccg cgagcaggcg tcgagctggg
     2101 actgctgggc cgagccgctg gtgggcgtgg ccatcgatcc gcagcacgtg tccgatt
//
LOCUS       DASZZC010000142         5855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_589235,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000142 DASZZC010000000
VERSION     DASZZC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589235"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..811)
                     /locus_tag="VHE37_03325"
     CDS             complement(119..811)
                     /locus_tag="VHE37_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HVT34589.1"
                     /translation="MNATTMTVTEALQRRTSIRAFLPTPVADATVREILDVARYAPSG
                     GNLQPWKVVVVSGTARDAVVALAKNYPGMFPAEDAERPVYPPNLWEPYRSRRYQVGED
                     MYALLGIARDNKPARLKHVARNFEFFGAPVGVFYVIDERMGHGQWAHLGMYMQSVALA
                     ATERGLGTCFQEFWGTLRATLKKHFALPEHDMIYCGMALGHADPAAPVNRLRSARAPL
                     EEFARFENGAPS"
     gene            complement(817..2388)
                     /locus_tag="VHE37_03330"
     CDS             complement(817..2388)
                     /locus_tag="VHE37_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34590.1"
                     /translation="MSTRRAFLKTAAGAAVALNLPGCARSVAPAADAKSAMASADELR
                     AMVQNMVDMGPRLTGSSAHNRFIDWLEAQMQALGMQVIRDPYSFTRWQAREWSLAVLE
                     ADGNIPIGTAGYFPYSGSTGGAGIEGELVSIDLPIEDITANITQPADLVQFVSTLSQA
                     LLPSLQAALGAVQGGVSGAIVLVNGLVPPALTGIFAPFLSYAKSSDLATLLTSDYKRT
                     WLGGLLFANLLDPIRQAGAAGAIFNIDASPECARGQYLPFSSPLNTLPALLVDRVVGA
                     DLRARAAARPTVRLILVADTFPDTRSDSLVGILPGASDENMILNTHTDGQNAFEENGG
                     IACIAIARHYAAIPQAQRPRALVFSLVTGHMGPDLPQTQGFVDRFPDLIAKAACGITI
                     EHFGATNWEDDAAGYHATGSPEFGGAFHSVTPVGDIAAQAYQASPLSGIALLRPIGNT
                     YFGVGAPLHQAGVPSIGYLTGPNYLVTISANGEMDKLDPQRMQQETAWIIDTLHRLET
                     VPAATLKQGVGLPGI"
     gene            complement(2385..3815)
                     /locus_tag="VHE37_03335"
     CDS             complement(2385..3815)
                     /locus_tag="VHE37_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HVT34591.1"
                     /translation="MPDIQKIQRHADALWEDEIVGQLTEYIRIPNKSPMFDPQWAEHG
                     YMDDAVKLMEKWARGKLPALPGAVLEVVRLPGRTPLIFIDVPAHGVAKSDDCVMLYGH
                     LDKQPEMTGWADGYGPWSPRRVGDKLYGRGGADDGYAIFGSLAALLALREQNIPHARC
                     VVLIEACEESGSYDLPFYVDHLMPRLGKPSLVVCLDSGCGNYEQLWLTTSLRGLTGGN
                     LKVQVLSEGVHSGDASGVVASSFRILRQLLSRLEDETSGAIKPKDFYVDIPPQRIEQA
                     RAVAQVLGNDVYGKFPFVAGMRPVSSELSELVLNRTWRPALSVTGVDGMPPLASAGNV
                     LRPHTAVKLSLRIPPTADGARCGAALKKLLESDPPYGCKVTLELEKASSGWNAPQLQP
                     WLEQAVAQASQNYFGKPPAYMGEGGSIPFMGMLGEKFPAAQFLITGVLGPHSNAHGPN
                     EFLHIPMGKRVTMVVADVVARHGARA"
     gene            complement(4150..5163)
                     /locus_tag="VHE37_03340"
     CDS             complement(4150..5163)
                     /locus_tag="VHE37_03340"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HVT34592.1"
                     /translation="MKDETITTSTPVDVPVVTRRALQAALTREEIAALTARSDWRGAL
                     AIARSWLIIAGAFALAAAWPNPLTIVVAMILIAGRQLALAVLEHEGAHGTLFKTRWMN
                     DVLCDWLCARPVWQNLKKYRAHHLVHHTHTGSDQDPDLSLHAGYPVSRASMRRKLLRD
                     VSGYTGVKLIIGLVLMDAGYFKWTVSNHVEKLPQDGRRWWNYGLDFLRNASGMLAANL
                     ALYAVLAASGHGWLYLLWVGAYLTPFPLLVRVRSIAEHGVLPRVPDMLRNTRTTRATP
                     IERLLWAPLQVNYHVEHHAMASVPYYRLPQLHALLRARGVLGPAPDYFDVLRLASTPA
                     AAH"
     gene            complement(5188..>5855)
                     /locus_tag="VHE37_03345"
     CDS             complement(5188..>5855)
                     /locus_tag="VHE37_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HVT34593.1"
                     /translation="EAAGRGAYRLGAAAMQLAGEVAKWREGEARVRPWNGDWLAVHTA
                     PLGRRDRAAARTRDRALDLLGLRELDPGLYLRPDNLAGGAARARERLLALGLEAQACV
                     FVASAFDAAREQRARKLWDGAALTRRYLQGRARLDSWLARADELDHEVAARESYRLGD
                     AAIRELVFDPLLPDPLVDTRARAAYTQAMLKFDRAGHAIWRTLAQPPATGRDARIGVR
                     RTH"
BASE COUNT          925 a         1967 c         2051 g          912 t
ORIGIN      
        1 cagccgaagc gccgcgccca ggtgagcggc atgaggaaga tgggcagcgc gaacactgca
       61 ctggtgatga cgacgatcac gacgagaaaa atctgcgcgt gcgcgcccca ggcgccgatc
      121 acgatggcgc cccgttttca aagcgcgcga actcctccag cggcgcccgt gcgctgcgca
      181 ggcggttgac cggggcagcc gggtcggcgt ggcccagcgc catgccgcaa tagatcatgt
      241 cgtgctcggg cagcgcgaaa tgttttttca gcgtggcgcg cagcgtgccc cagaattcct
      301 ggaagcaggt gccgagcccg cgctcggtgg cggccagcgc caccgactgc atgtacatgc
      361 ccaggtgcgc ccactggccg tggcccatgc gctcgtcgat cacgtagaac acgccgaccg
      421 gcgcgccaaa gaattcaaag ttgcgcgcca cgtgcttgag ccgcgccggc ttgttgtcgc
      481 gcgcgatgcc gagcagcgca tacatgtctt cgcccacctg gtagcggcgg ctgcggtacg
      541 gctcccacaa gttgggcggg tacacgggcc gctcggcatc ctcggcggga aacatgcccg
      601 gataattttt tgccagtgcg accaccgcat cgcgcgcggt gcccgatacc acgacgactt
      661 tccacggctg caggttgcca cccgagggcg cgtagcgcgc cacatcgaga atctcccgca
      721 ccgtcgcgtc cgccaccggc gtgggcagga aggcgcggat cgaggtgcgg cgctgcagcg
      781 cttcggtgac ggtcatggtc gtggcattca tggtggtcat attcccggca atcccacgcc
      841 ctgcttgagc gtggccgccg gcacggtctc caggcgatgc agcgtgtcga tgatccaagc
      901 ggtttcctgc tgcatgcgct gcgggtcgag cttgtccatc tcgccattgg cgctgatggt
      961 caccaggtaa ttcggcccgg tgaggtagcc gatgctcggc acgccggcct ggtgcagcgg
     1021 cgcgccgacg ccgaaatagg tatttccaat cggccgcagc agcgcgatgc cgctgagcgg
     1081 gctggcctgg taggcctgcg cggcgatgtc gccgaccggc gtgaccgagt ggaacgcgcc
     1141 gccgaattcc ggcgagccgg tcgcgtggta gccggccgcg tcgtcttccc agttggtggc
     1201 gccgaaatgc tcgatggtga tgccgcaggc ggccttggcg atcagatccg ggaagcggtc
     1261 aacgaagccc tgcgtctgcg gcaggtccgg ccccatgtgg ccggtgacca ggctgaacac
     1321 cagcgcgcgc ggacgctgcg cctgcgggat cgccgcgtaa tggcgcgcga tggcgatgca
     1381 ggcgatgccg ccgttttcct cgaacgcgtt ctgcccgtcg gtgtgcgtgt tgagaatcat
     1441 gttctcgtcg ctggcgcccg gcaggatgcc gaccaggctg tcgctgcgcg tgtcgggaaa
     1501 agtgtcggcg accaggatca ggcgcacggt cggccgcgcc gccgcgcgtg cgcgcaagtc
     1561 ggcgccgacc acgcggtcga ccagcagcgc gggcagcgtg ttcagcggcg aggagaacgg
     1621 caggtattgg ccgcgcgcgc actcgggcga ggcatcgatg ttgaaaatgg cgccggccgc
     1681 gcccgcctgg cggatcgggt cgagcaggtt ggcgaacagc agcccgccga gccaggtgcg
     1741 cttgtaatcg ctggtcagca gcgtggccag gtcgctgctt ttggcatagc tgagaaacgg
     1801 cgcgaagatg ccggtgagcg cgggcggcac cagcccgttg accagcacga tggcgccgga
     1861 cacgccgccc tgcacggcgc ccagcgcggc ctgcagcgac ggcagcagcg cctgcgacag
     1921 cgtcgacacg aactgcacca ggtcggccgg ttgcgtgatg ttggcggtga tgtcctcgat
     1981 cggcaggtca atcgacacca gttcgccctc gatgcccgcg ccgccggtgc tgccggagta
     2041 cggaaaataa ccggccgtcc cgatgggaat gttgccgtcc gcttcgagca ccgccagcga
     2101 ccactcgcgc gcctgccagc gggtgaagct gtagggatcg cggatcacct gcatgcccag
     2161 cgcctgcatc tgcgcttcca gccagtcaat gaacctgttg tgcgcggacg agccggtgag
     2221 gcgcgggccc atgtccacca tgttctgcac catggcgcgc agctcgtcgg cgctggccat
     2281 ggcgcttttc gcatccgccg ccggtgccac gctgcgcgcg cagccgggca ggttgagcgc
     2341 caccgcggcg ccggccgccg ttttcagaaa cgcgcgccgc gtgctcatgc gcgcgcgccg
     2401 tgccgcgcca caacgtcggc caccaccatg gtcacgcgct tgcccatggg gatatgcagg
     2461 aattcattcg gcccgtgcgc gttggagtgc gggccgagca cgccggtgat gaggaactgc
     2521 gcggcgggga atttctcgcc gagcatgccc atgaatggaa tgctgccgcc ttcgcccatg
     2581 taggccggcg gcttgccgaa ataattctgc gaggcctgcg ccaccgcctg ttccagccag
     2641 ggctgcagct gcggcgcgtt ccagcccgac gaggccttct ccagttcgag cgtcaccttg
     2701 cagccgtagg gcgggtcgct ctccagcaat ttcttcagcg cggcgccgca gcgcgcgccg
     2761 tcggcggtgg gcggaatgcg cagcgacagt ttcaccgcgg tgtgcggacg cagcacgttg
     2821 ccggcggagg ccagcggcgg catgccgtcc acgccggtga ctgacagcgc cgggcgccag
     2881 gtgcggttga gcaccagttc agaaagttcg ctgctcaccg ggcgcattcc ggctacgaag
     2941 ggaaacttgc cgtacacgtc gttgcccagc acctgcgcca ccgcgcgcgc ctgctcgatg
     3001 cgctgcggcg ggatgtcgac gtaaaaatcc ttcggcttga tcgcgccgct ggtttcgtcc
     3061 tcgagccgcg acagcagctg gcgcaggatg cggaagctcg aggccaccac gccggacgca
     3121 tcgccgctgt gcacgccctc gctcagcacc tgtactttca gattgccgcc agtcagcccg
     3181 cgcaggctgg tggtgagcca cagctgctcg tagttgccgc agccggaatc gaggcagacc
     3241 accagcgacg gcttgcccag gcgcggcatc aggtgatcga cgtagaacgg caggtcgtag
     3301 ctgcccgatt cctcgcaggc ctcgatcagc accacgcagc gcgcgtgcgg aatgttctgc
     3361 tcgcgcagcg ccagcagcgc ggccagcgag ccgaagatgg cgtaaccgtc gtcggcgccg
     3421 ccgcggccat agagcttgtc gccgacgcgg cgcggcgacc acgggccgta gccatcggcc
     3481 cagccggtca tctccggctg cttgtcgaga tggccgtaga gcatcacgca gtcgtcggac
     3541 ttcgccacgc catgcgcggg cacgtcgatg aaaatcagcg gcgtgcggcc gggcaggcgc
     3601 accacttcga gcaccgcgcc gggcagcgca ggcagctttc ctcgcgccca tttctccatc
     3661 agcttcaccg cgtcgtccat atagccgtgt tcggcccatt gcgggtcgaa catcggcgac
     3721 ttgttgggga tgcggatgta ctcggtcagc tggccgacga tttcgtcttc ccacagcgcg
     3781 tcggcgtggc gctggatttt ctggatgtca ggcatggcga gttccgttgc gcgggcgcgc
     3841 agtgtagcgc gcgcgttgcg gcgcttcgcc gggcggcgtg ccgcggagaa aaacaggtca
     3901 ccgcatcggg ttttgcgatg ttatataaaa tagtattatt ataaatatat acaatattaa
     3961 ctaacatcat ggatggcggt gcggtgagat cattttccgg gcacacgcgc aaaaaaagac
     4021 gcccgcgcga ggcgggcgtc cacggaagcg ggccgatggg tgggggagcc cgcttggtgc
     4081 cggtactgtg gagatcagcg ccgacaacca caggctaagg cgcggcgcgc ggccgcgcat
     4141 tgatccaggt caatgcgccg cggcgggcgt cgaggccagg cgcagcacgt cgaaataatc
     4201 cggcgcaggt ccgagcacgc cgcgcgcgcg cagcagcgca tgcagctgag gcaaccggta
     4261 atacggcacc gaggccatgg cgtgatgctc cacgtggtag ttcacctgca gcggcgccca
     4321 cagcaggcgc tcgatgggcg tggcgcgcgt ggtgcgcgtg ttgcgcagca tgtccggcac
     4381 gcgcggcagc acgccgtgct cggcgatcga gcgcacgcgc accagcagcg ggaacggcgt
     4441 gagatatgcg cccacccaca gcaggtacag ccagccatgc ccgctggcgg ccagcaccgc
     4501 gtacagcgcg agattggccg cgagcatgcc ggatgcgttg cgcagaaaat cgagcccgta
     4561 gttccaccag cggcgcccgt cctgcggcag tttctcgacg tggttggaca cggtccactt
     4621 gaagtagccg gcatccatca gcaccaggcc gatgatcagc ttcacgccgg tgtagccgct
     4681 gacgtcgcgc agcagcttgc gccgcatcga cgcgcgcgac accgggtagc cggcatgcag
     4741 cgagaggtcc gggtcctggt cgctgccggt gtgcgtgtgg tgcaccaggt gatgcgcgcg
     4801 gtacttcttc aggttctgcc acaccgggcg ggcgcacagc cagtcgcaca gcacatcgtt
     4861 catccagcgg gtcttgaaca gggtgccgtg cgcgccctcg tgctccagca ccgcgagcgc
     4921 gagctggcgg ccggcgatca ggatcatggc gacgacgatg gtcagcggat tgggccaggc
     4981 cgcggccagc gcgaaggcgc cggcgatgat cagccacgag cgggcgatgg ccagcgcgcc
     5041 gcgccagtcc gagcgtgcgg tcagcgcggc gatctcctcg cgcgtgagcg ccgcctgcag
     5101 cgcgcgccgg gtcaccacgg gtacgtcgac aggcgtggac gtggtgatgg tttcatcttt
     5161 catgtgcatc tccaggacat caggacatca atgcgtgcgg cgaacgccaa tgcgcgcgtc
     5221 gcgccccgtt gcgggcggct gtgccagcgt gcgccagatg gcgtggccgg cgcggtcgaa
     5281 cttcagcatc gcctgcgtgt aggcggcgcg cgcgcgcgtg tcgaccagcg gatcgggcag
     5341 cagcgggtcg aacaccagtt cgcggatggc ggcatcgccc aggcggtacg actcgcgcgc
     5401 ggccacttcg tgatcgagct cgtcagcgcg cgccagccag ctgtccagcc gcgcgcgtcc
     5461 ctgcaggtag cggcgggtga gcgcggcgcc atcccacaac ttacgcgcgc gctgctcgcg
     5521 cgcagcgtcg aaagcgctgg cgacgaacac gcaggcttgc gcttccagcc ccagcgcgag
     5581 caggcgctcg cgcgctcgcg ccgcgccgcc ggcaaggttg tccgggcgca ggtacaggcc
     5641 gggatcgagc tcgcgcaggc cgagcaggtc cagcgcgcgg tcgcgggtgc gcgcggccgc
     5701 gcggtcgcgc cggcccagcg gcgcggtgtg caccgccagc cagtcgccat tccatggccg
     5761 cacgcgcgcc tcgccttcac gccacttggc cacttcgcct gccagttgca tggccgccgc
     5821 accgagccgg tacgcgccgc gcccggcggc ctcga
//
LOCUS       DASZZC010000143         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_911816,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000143 DASZZC010000000
VERSION     DASZZC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911816"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHE37_03350"
     CDS             <1..201
                     /locus_tag="VHE37_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34594.1"
                     /translation="GLDGTEVFDIEGLKKGAREVTVKAKKADGKTVSFDAKVRINTPK
                     EWEYYENGGVLQYMLRQMARSA"
     gene            566..1375
                     /locus_tag="VHE37_03355"
     CDS             566..1375
                     /locus_tag="VHE37_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT34595.1"
                     /translation="MKLLLRALRFAAVMAITGFLVAAAVLWFAQRSMIFPRIDARGQQ
                     ARPADAQQVWLDIPGGRVEAWYLPPAEPHDARAPLIIFAHGNGEFIDRWPPAFDEPRR
                     WGVGALLVEFPGYGRSTGAPSQDTVTAAMLAAWSWAAAQPGIDAQRIVAYGRSLGGGA
                     VCTLATQRAPAALILESTFTSVRIFAARYFMPGFTVRDPFDNLACVQNWRGPLLVLHG
                     DHDTLIPQAEGRALAAAAPQSEFVSLDCGHNDCPRSWPRIREFLAAHGVLH"
     gene            complement(1442..3442)
                     /locus_tag="VHE37_03360"
     CDS             complement(1442..3442)
                     /locus_tag="VHE37_03360"
                     /inference="COORDINATES: protein motif:HMM:NF023643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N4-asparagine amidase"
                     /protein_id="HVT34596.1"
                     /translation="MNACSRAALLALACVCADAHAVPTSLTDLPQLTAEAPPRVPATA
                     SCTEKVLSHLFVNSYGTPGFGAHAAAACPGPWSAVILSIDVSVSGVQFDRIFDIYAGR
                     YLLLSSSTSEPASEVPNALTHWHIDTDVSRYAGLLAGDQPITAILNNINDATYTGQYQ
                     VDLSLTFYATDANTPAAKVPDWLMPVLPASTDPAHPDGPGNYGDDGYSPLRPATPTYT
                     QSVTLPQNLLALYADVYAQGHGACEEFWWAAPGQCGVGTPLRQVVLSIDGVIAGFAPV
                     YPVLFTGGGGPGSWNPIPSPRAWHLDPYRLDLTPFVGRLVDGQPHSIRLDVPDAGYSD
                     PGDFWEVGAVLSGTLDAASTQTSGALTSAPVADAATEMIMGDATNTGIIDFSASRSGE
                     WIGYVTGSGGRVDTEVTSNFDLSTVSAVALTDSTWHWTTVSRNAVNGATPVSMTTART
                     YQLQAPPAGGGHFEDAATVSVDGAAPMQSNFDLNLYTLGAVEANVQQVEHYTASDSSG
                     YCYDHLVTANAGYVVADQVLPCISLPGVGTGSGTGSTTGSSTGSTTGSTTGGDTGGST
                     TGSSTGSTTGDSTGSSTGSSTGGTTGTSTGATTGDATGSSTGSTSGGSTGTAQPADNS
                     AGGGGGALSPLMLLVMLVGAGRRLPRGFTASIKSMLLIVYRI"
     gene            3719..3844
                     /locus_tag="VHE37_03365"
     CDS             3719..3844
                     /locus_tag="VHE37_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34597.1"
                     /translation="MRVPEMPACSRRAMLPLWGFLIIGLAAFAGLLLYVIFCDRF"
     gene            3850..3939
                     /gene="kdpF"
                     /locus_tag="VHE37_03370"
     CDS             3850..3939
                     /gene="kdpF"
                     /locus_tag="VHE37_03370"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HVT34598.1"
                     /translation="MTFDLILGGFAVAFLLVYLTYALLRPEKF"
     gene            3950..>4609
                     /locus_tag="VHE37_03375"
     CDS             3950..>4609
                     /locus_tag="VHE37_03375"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HVT34599.1"
                     /translation="MTFNGFLQIILYFALLTALTRPLGGYMYRVYAGERTWLDAVLGP
                     IERAFYRLAGVDATREHGWAAYTLGMLTFNLLGFVAVYLVLMFQDHLPWNPQHLPGLS
                     AHLAFNTAVSFTTNTNWQSYGGESTLSYFSQMAALTVQNFVSAATGMALAIALVRGIA
                     RKSAATVGNFWVDLTRTTLYILLPLSLAFALVLIWQGVPQNLHAYVDATTVEGAKQSI
                     AQ"
BASE COUNT          689 a         1536 c         1509 g          875 t
ORIGIN      
        1 gggctcgacg gcacggaagt gttcgacatc gagggcctga agaagggcgc ccgggaagtc
       61 acggtgaagg cgaagaaggc cgacggcaag accgtgagct tcgacgccaa ggtgcgcatc
      121 aacacgccca aggagtggga gtactacgag aacggcggcg tgctgcagta catgctgcgg
      181 cagatggcgc gcagcgcgta agcgatgacg gtttgaaagc aagggcgctt cggcgccctt
      241 gtttttgggt gctgcgcttc ggcgcccttg ttttttggta tcgcgcttcg gtgctcgtac
      301 ttttggtcga gccgctcacc aaaagtgttg acgtgccatt ccttctcccc tcgggagacg
      361 gtggcgcgca gcgccggatg agggaggcgc gcgcatccgg ccacagggcc gcagcgagtc
      421 cgtccctcac ccgcgactgc ggcgcggcct ctcccggcgg gagaggcacg caagcttccg
      481 cgggagcttt caacacctgg actgagcaac tcggcttttg cccgcttttt ctctgcacgt
      541 ttttttgctg cggcatactg cgccgatgaa actcctgttg cgcgcactgc gcttcgccgc
      601 ggtcatggcg attaccggct tcctcgtcgc cgccgccgtg ctgtggttcg cgcagcgctc
      661 gatgatcttc ccgcgcatcg acgcgcgcgg ccagcaggcg cgcccggccg atgcgcagca
      721 ggtatggctc gacatccccg gcggccgcgt cgaggcgtgg tacctgccgc cggcggagcc
      781 gcacgatgcg cgcgcgccgc tgatcatctt cgcccacggc aacggcgagt tcatcgaccg
      841 ctggccgccg gcgttcgacg agccgcggcg ctggggcgtg ggcgcgttgc tggtggaatt
      901 tcccggctac ggacgctcca ccggcgcgcc gtcgcaggac acggtgactg cggcgatgct
      961 cgccgcctgg agctgggctg ccgcgcagcc cggcatcgac gcgcagcgca tcgtcgccta
     1021 cggccgctcg ctcggcggcg gcgcggtgtg cacgctggcg acgcagcgcg ctcctgcggc
     1081 gctgatcctg gagtcaacct tcaccagcgt gcgcatcttc gccgcgcgtt acttcatgcc
     1141 gggattcacc gtgcgcgatc cgttcgacaa tctcgcctgc gtgcagaact ggcgcggacc
     1201 gctgctggtg ctgcacggcg atcacgacac gctcatcccg caggcggaag gccgcgcact
     1261 ggcggcggca gcaccgcaat cggaattcgt ttcactcgat tgcggccaca acgattgtcc
     1321 gcgcagctgg ccgcgcatcc gcgaatttct cgccgcgcat ggtgtgctgc attgagtggt
     1381 ggcggcacaa gaatcactcc accgcacagc cgttttgcgt gttattaatt gttgattatt
     1441 tttatattcg ataaacaatt aataacatcg attttatcga tgcggtgaac ccgcgcggca
     1501 ggcgtctgcc tgctcccacc agcatgacca gcagcatcag cggcgacagc gcgccaccac
     1561 cgccgcccgc gctgttgtcc gccggttgcg ccgtgccggt gctgccgccg gatgtgctgc
     1621 ccgtgctgct gcccgtcgcg tcgccggtcg ttgcgccggt ggaagttccg gtggtgccgc
     1681 cggtcgaact gccagtgctg cttcccgtgc tgtcgcccgt cgtgctgccg gtcgaacttc
     1741 ctgtggtgct gccgccggta tcgccgccgg tcgtcgatcc ggtcgtgctg cccgtcgaac
     1801 tcccggtcgt gctgccggtg cccgagccgg tgccgacacc tggcagcgag atgcacggca
     1861 gaacctggtc tgccacgacg tagcccgcat tcgccgtcac caggtggtcg tagcaatagc
     1921 cgctgctgtc gctggcggtg taatgctcga cctgctgcac gttggcttcc accgcgccga
     1981 gcgtgtagag attcagatcg aagttcgatt gcatcggcgc tgcgccatcg acgctgaccg
     2041 ttgccgcatc ttcaaaatgt ccgccgcccg cgggcggtgc ctgcagctgg taggtgcgcg
     2101 cagtcgtcat gctcactggc gtggcgccgt tcactgcatt gcgcgacacc gtggtccagt
     2161 gccaggtgct gtcggtgagg gcgacggcgc tgacggtgct gagatcgaaa ttgctggtca
     2221 cttccgtatc gacgcgcccg cctgaacccg tcacgtagcc gatccactcg ccgctgcgcg
     2281 atgcgctgaa gtcgatgatg ccggtgttgg tggcatcgcc catgatcatt tccgtcgcgg
     2341 cgtccgccac cggcgccgac gtgagagcgc cgctggtctg cgttgatgcc gcatcgagcg
     2401 tgcccgacag caccgcgccc acttcccaga aatcgcccgg atcggaatag ccggcatcgg
     2461 gcacgtcgag cctgatgctg tgcggctggc catcgaccag gcggcccacg aacggcgtga
     2521 ggtcgagccg gtacggatcg agatgccagg cgcgcggcga cggaatcggg ttccacgagc
     2581 ccggtccgcc gccaccggtg aacagcaccg ggtacaccgg cgcgaagccg gcgatgacgc
     2641 cgtcgatcga cagcaccacc tggcgcagcg gcgtgccgac gccgcactgc cccggcgcgg
     2701 cccaccagaa ttcctcgcag gcgccgtgac cctgcgcgta cacgtcggcg tagagcgcga
     2761 gcaggttctg cggcagcgtc acgctctgcg tatacgtggg cgtcgcggga cgcagcggcg
     2821 agtagccgtc gtcgccgtaa ttgcccgggc catcgggatg cgcaggatcg gtcgacgccg
     2881 gcaggacggg catcagccag tccggcactt tcgccgcggg cgtgttcgca tcggtcgcgt
     2941 aaaacgtgag cgacaggtcc acctggtact gcccggtgta ggtggcgtcg ttgatgttgt
     3001 tgaggatggc ggtgatcggc tggtcgcccg ccagcagccc ggcgtagcgg ctgacgtcgg
     3061 tgtcgatgtg ccagtgcgtc agtgcattgg gcacttcgct cgccggctcc gaggtggagc
     3121 tcgacagcag caggtagcgg ccggcgtaga tgtcgaagat gcggtcgaac tgcacgccgc
     3181 tcaccgagac gtcgatcgac aggatcaccg ccgaccacgg gcccgggcac gccgccgccg
     3241 cgtgcgcgcc gaagcccggc gtgccgtagg aattaacgaa caggtgcgac agcactttct
     3301 ccgtgcagct cgcggtggcc ggcacgcgcg gcggcgcctc ggcggtgagc tgcggcagat
     3361 cggtgaggct ggtgggcacc gcgtgcgcat cggcgcagac gcaggccagc gcgagcagcg
     3421 cggcgcgaga gcaggcgttc atggtttctc cgtcagcaca tttcacaccg cggacgccgc
     3481 gcggcgcatg aggaacttcc cttgcgccgc cagcgaatca gcacgttttt ttacctcacc
     3541 gcaccgccat ttctcatgtt attaactatt gtatgttttt atatttaata agcatttaat
     3601 aacatcgaag atggcagtgc ggtggagcgg tttttttgcc tttcgccgtc gtttttatgg
     3661 attttttatt tttcgagccg cgcgccccgc tcgaatccct atgcgctcgc ggcgttgaat
     3721 gcgtgtgccg gaaatgccgg cttgttcgag acgcgccatg ttgccgctct ggggtttcct
     3781 gatcatcggg ctcgccgcct tcgccggcct gttgctctac gtgattttct gcgaccggtt
     3841 ctgaacacca tgaccttcga tctcatcctc ggcggcttcg cggtggcctt cctgctggtc
     3901 tacctcacct acgcgctgct gcgcccggaa aaattctgag acacgcgcca tgaccttcaa
     3961 cggcttcctg cagatcatcc tgtacttcgc cctgctcacg gcgctcacgc gcccgctggg
     4021 cggctacatg taccgcgtgt acgcgggcga gcgcacctgg ctcgatgcgg tgctcggccc
     4081 gatcgagcgc gcgttctatc gcctggccgg cgtcgacgct acgcgcgagc acggctgggc
     4141 cgcgtacacg ctcggcatgc tcaccttcaa cctgctcggc ttcgtcgcgg tgtacctggt
     4201 gctgatgttc caggatcacc tgccgtggaa cccgcagcac ctgcccgggc tgtcggcgca
     4261 tctcgccttc aacaccgccg tctcattcac caccaatacc aactggcaga gctacggcgg
     4321 cgaaagcacg ctctcctact tctcgcagat ggcggcgctg acggtgcaga acttcgtgtc
     4381 cgccgcaacc ggcatggcgc tggcgattgc gctggtgcgg ggcatcgcgc gcaagtcggc
     4441 ggccaccgtc ggcaacttct gggtcgacct cacccgcacc acgctgtaca tcctgctgcc
     4501 gctgtcgctc gccttcgcgc tggtgctgat ctggcagggc gtgccgcaga acctgcatgc
     4561 gtacgtcgac gccaccacgg tggaaggcgc gaagcagagc atcgcgcag
//
LOCUS       DASZZC010000144         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1167053,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000144 DASZZC010000000
VERSION     DASZZC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167053"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VHE37_03380"
     CDS             complement(<1..996)
                     /locus_tag="VHE37_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVT34600.1"
                     /translation="MSALLSPRRSIAGICFTLAAATLAAACLALMQGPLHIAPTQVWH
                     SLWHAPDDSLESATVLLVRLPRVLLALVIGAALAQAGASMQAIFRNPLADPGLAGVSS
                     GAALGAALVIVLAHRADAGDGTLVRWLLPAAAFAGGAGTAWLVSRLAQVDGTTRVGTM
                     LLAGLAINAIAGAGLGFLSHIASDTSLHTLSFWMFGSLAKSGWNEIALLAPLLLAATL
                     LIPRDARALDALLLGEVEAGHLGIDVERLKRRQLVLIVFCASASAALGGIIGFVGLLV
                     PHLVRRWAGPDHRLLLPVSALGGALLLLLADTAARVLLAPAELPIGIFTALLGGPF"
     gene            1163..1244
                     /locus_tag="VHE37_03385"
     tRNA            1163..1244
                     /locus_tag="VHE37_03385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1197..1199,aa:Leu,seq:tag)
     gene            1362..>2293
                     /gene="tig"
                     /locus_tag="VHE37_03390"
     CDS             1362..>2293
                     /gene="tig"
                     /locus_tag="VHE37_03390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HVT34601.1"
                     /translation="MEVNVENSDGLSRQLRVRVPAERVAKAVDERLKRVASRARVPGF
                     RPGKAPFKVIEQQYGESARLEAVSDLVQQTYPEALGKAGVHPAGAPKIDITAERPGQP
                     LEYVAKFEVYPEIKLANLSAIAVEKPVVEIADTDIDKLVDNLRRNRRTFETVTRAAAA
                     GDTVTLDFEGKLDGTVFPGGQGKDVSFEIGESRFLPDLENGIVGHAAGESFTVDVKFP
                     EDYRAENLRGKTAQFDVQLKEVKEPKLPPIDAEFLKSHQVEESAGEAGLREKCRAALV
                     KERDKAVSNSVKQQALDQLLAHNPIEIPQALIEQ"
BASE COUNT          451 a          761 c          781 g          300 t
ORIGIN      
        1 gaacggcccg ccgagcaacg cggtgaagat cccgatcggc agttccgccg gcgccagcag
       61 cacgcgtgcg gcggtgtcgg ccagcagcag cagcagcgcg ccgcccagag ccgacaccgg
      121 cagcagcagc cggtgatccg gcccggccca gcgccgcacc aggtgcggaa ccagcaggcc
      181 gacgaagccg atgatgccgc ccagcgccgc gctggccgag gcgcagaaca cgatcagcac
      241 cagctgccgc cgcttgagcc gctcgacatc gatgcccagg tggccggctt ccacttcgcc
      301 cagcagcagc gcatcgagcg cgcgcgcgtc gcgcgggatc agcagcgtgg cggccagcag
      361 cagcggcgcc agcagcgcga tttcattcca gccggacttg gccagcgaac cgaacatcca
      421 gaacgacagc gtgtgcagcg aagtgtcgct ggcgatgtgg ctgaggaacc ccaggcccgc
      481 gccggcgatg gcgttgatcg ccagccccgc cagcagcatg gtgccgacgc gcgtggtgcc
      541 gtccacctgc gccagcctcg acaccagcca cgcggtgccg gcgccgccgg cgaacgcggc
      601 ggcgggcagc agccagcgca ccagcgtgcc gtcgcctgca tccgcgcgat gcgcgagcac
      661 gatgaccagc gcggcgccga gcgcggcgcc gctcgacacg ccggccagtc ccggatcggc
      721 cagcgggttg cggaagatgg cctgcatgct cgcgcccgcc tgcgccagcg ccgcgccgat
      781 gacgagcgca agcagcacgc gcggcaggcg caccagcagc accgttgcac tttccaggct
      841 gtcgtccggg gcgtgccaca ggctgtgcca cacctgcgtc ggcgcgatgt gcagcgggcc
      901 ctgcatgagt gccaggcagg cagcggcaag cgtagcggcc gcaagcgtga agcagatgcc
      961 ggcaatggag cgccgcggcg acagcagggc gctcatgagg ccttggaagc ggctgaccgc
     1021 accggcattt tgggcgttat taaagatcca ctaattttat atatattcaa catttaataa
     1081 catccaaatg ccggatgcgg tggagcaaaa aagccaggcc ggcagaatag cgaaagttgc
     1141 cgctgatata cttcgcgccc tcgcgaaagt ggcggaattg gtagacgcac cagatttagg
     1201 ttctggcgcc gcgaggcgtg cgagttcgag tctcgccttt cgcacaagac aagttctttt
     1261 cacggagcgc agcgcagtgt ccctgttctc tctccccttg cgggagagat aaaagagagg
     1321 ggggaggcgt caagcttcag caacagtccg aagagcaaac catggaagtc aacgtagaaa
     1381 actccgacgg cctgagccgt caactgcgcg tccgcgtgcc cgccgagcgc gtggccaagg
     1441 cggtcgacga gcgcctcaag cgcgtcgcca gccgtgcgcg cgtgccgggc ttccgcccgg
     1501 gcaaggcgcc gttcaaggtg atcgagcagc agtacggcga gtccgcgcgc ctcgaggccg
     1561 tttccgacct ggtgcagcag acctaccccg aagctctcgg caaggccggt gtgcatccgg
     1621 ctggcgcgcc gaagatcgac atcaccgccg agcgccccgg gcagccgctg gaatacgtgg
     1681 ccaagttcga ggtgtacccg gagatcaagc tggcgaacct gtcggcgatc gccgtcgaaa
     1741 aacccgtcgt ggagattgcc gacaccgaca tcgacaagct ggtggacaac ctgcgccgca
     1801 accgtcgcac gtttgaaacg gtgacgcgcg ccgccgcagc cggcgatacc gtaacgctgg
     1861 acttcgaagg caagctcgac ggcaccgtgt tcccgggcgg gcagggcaag gatgtgagct
     1921 tcgagatcgg cgagtcacgc ttcctgccgg acctggaaaa cggcatcgtc ggtcatgccg
     1981 ccggcgagag cttcaccgtc gacgtgaaat tccccgagga ctaccgcgcg gaaaacctgc
     2041 gcggcaagac ggcgcagttc gacgtgcagc tcaaggaagt aaaggagccg aagctgccgc
     2101 cgatcgacgc cgagttcctc aagtcgcacc aggtcgagga gagcgcgggt gaagccggcc
     2161 tgcgcgagaa gtgccgcgcg gcgctggtca aggagcgcga caaggcggtg tcgaacagcg
     2221 tgaagcagca ggcgctggac cagctgctgg cgcacaaccc gatcgagatt ccgcaggcgc
     2281 tgatcgagca gga
//
LOCUS       DASZZC010000145         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1167144,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000145 DASZZC010000000
VERSION     DASZZC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167144"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VHE37_03395"
     CDS             <1..766
                     /locus_tag="VHE37_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT34602.1"
                     /translation="AGRMFTPGLREVIAGKAAQQQFQGVELGRTLKFRGSEWTVVGTF
                     STDGDAHETELWTDLDTARGAFGRPGASSVLARLTAPAALGGLQKAMAADPRFQLDAQ
                     SEKAYYAAQSEGVTQNIGIVTSVVTTIMALGALFGALNTMYSAVSTRQVEIATLRAIG
                     FSSLPVVVSVLSEAVLLAVAGGLLGAAVAWLAFNGMTVTTLAGGSFTQVAFSFLVTPG
                     LIGLGLGWAVSIGFIGGLLPAIRAARLPVVDALRAA"
     gene            763..1923
                     /locus_tag="VHE37_03400"
     CDS             763..1923
                     /locus_tag="VHE37_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT34603.1"
                     /translation="MKYLPLLWATLWRRRARTIYTMASIAVAFLLYGLLNGVNMAFSS
                     GVEAAGADRLMTAGKYSFTEILPLSYYQQIKNIPGVKAVTHATWFGGVYIDEHNFIPQ
                     FPVDQDTYFDVYPEMIIDPKQLQAFKDTRDGVLAGVELARRFGWKIGDRIPLQATIWP
                     NKQGGNDWSFQLVGLIDAKNEAARAQSEALFFRYDYFDEERQYGKGKVGWYIVRLDDP
                     KASDQIAAAIDKQFSNSANETRTDTEKAFNQSFIKQMGDIELIIRSILGAVFFTLLLL
                     TGNTMMQSVRERVPELAVLKTLGFTDGAVLALVLAESLLLCVFAALIGLLLSGLIMPG
                     LGAKAQMPGMQLTGALLASGCGVAVLLALVVGAVPATRAMRLQIVNALSGHT"
     gene            1940..>2784
                     /locus_tag="VHE37_03405"
     CDS             1940..>2784
                     /locus_tag="VHE37_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT34604.1"
                     /translation="MASFAAQIAAVTAMNVKNVPSRWGASMVIVIGIAGVVGVLIALL
                     SMARGFQATLASTGRADRAVLLRGGANDELSSVIMREQVDVVRQAPGVKKDAQGQPMA
                     LPEVYLLSGLPRRGGSEPSNVVVRGTEARVLEVRPEIRIVEGRMFTPGLREVVVGRGA
                     QAQFANLNLGDHAAIRNGDWAVVGIFESGGDVHESEVLADLETLMSAAHRPVPTSMVV
                     QLEDAAAFTRFKDALTADPRMSVKVLREPDYYSSRSEQLGAFINYLGYTVAVIMAIGA
                     LFGAL"
BASE COUNT          418 a          934 c          982 g          450 t
ORIGIN      
        1 ggccgggcgc atgttcaccc cgggtctgcg cgaggtgatc gccggcaagg cggcgcagca
       61 gcagttccag ggcgtcgagc tcggccgcac gctcaagttc cgcggctctg aatggaccgt
      121 ggtcggcacc ttctccaccg acggcgatgc gcacgagacc gaactgtgga ccgaccttga
      181 caccgcgcgc ggcgccttcg gccggcccgg cgcgtcgtcg gtgctggcca ggctcaccgc
      241 gccggccgcg ctcggcggac tgcagaaggc gatggcggcc gatccgcgct tccagctcga
      301 cgcacagagc gagaaggcct actacgcggc gcagtccgag ggcgtcaccc agaacatcgg
      361 catcgtcacc agcgtggtca ccaccatcat ggcgctgggt gcgctgttcg gcgcgctcaa
      421 caccatgtac tcggcggtgt cgacacggca ggtggagatc gccacgctgc gcgcgatcgg
      481 cttctccagc ctgccggtgg tggtgtcggt gctgagcgag gcagtgctgc tggcggtggc
      541 cggcggcctg ctcggcgcgg cggtcgcctg gctcgccttc aacggcatga ccgtgaccac
      601 gctggccggc ggttcattca cccaggtggc gttcagcttc ctggtgacgc cggggctgat
      661 cgggctgggc ctgggctggg cggtgagcat cggcttcatc ggcgggctgc tgccggcgat
      721 ccgcgccgcg cgcctgccgg tggtcgacgc gctgagagcg gcatgaaata cctgccgctg
      781 ctgtgggcca cgctgtggcg gcgccgcgcg cgcaccatct acaccatggc gtcgatcgcc
      841 gtggccttcc tgctgtacgg cctgctcaac ggcgtgaaca tggcgttctc ctccggcgtg
      901 gaagccgcgg gcgccgaccg cctgatgacc gcgggcaagt attcgttcac cgagatcctg
      961 ccgctgtcgt actaccagca gatcaagaac attcccggcg tgaaggcggt gacgcatgcc
     1021 acctggttcg gcggtgtgta catcgacgag cacaacttca tcccgcagtt tcccgtcgac
     1081 caggacacgt atttcgacgt gtacccggaa atgatcatcg acccgaaaca gctgcaggcg
     1141 ttcaaggaca cgcgtgacgg cgtgctggcc ggcgtcgagc tggcccggcg cttcggctgg
     1201 aagatcggcg accgcattcc gctgcaggcc accatctggc cgaacaagca gggcggcaac
     1261 gactggtcgt tccagctggt cgggctgatc gatgcgaaga acgaggcggc acgggcgcag
     1321 agcgaagcgc tgttctttcg ctacgactac ttcgacgagg agcgccagta cggcaagggc
     1381 aaggtgggct ggtacatcgt caggctcgat gacccgaagg cgtcggacca gatcgccgcc
     1441 gccatcgaca agcagttctc caactccgcc aacgagacgc gcaccgatac cgagaaggcc
     1501 ttcaaccaga gcttcatcaa gcagatgggc gacatcgagc tgatcatccg ctcgatcctg
     1561 ggcgcggtgt tcttcaccct gctgctgctc accggcaaca ccatgatgca gtcggtgcgc
     1621 gagcgcgtgc ccgaactggc ggtgctgaag acgctgggct tcaccgacgg cgccgtgctg
     1681 gcgctggtgc tggccgagtc gctgctgctg tgcgtgtttg ccgcgctgat cggcttgctg
     1741 ctgtcgggcc tgatcatgcc cgggctcggc gccaaggcgc agatgcccgg catgcagctc
     1801 accggcgcgc tgctggccag cggctgcggc gtggcggtgc tgctggcgct ggtcgtcggc
     1861 gccgtgccgg ccacgcgcgc catgcgcctg cagatcgtca acgccctctc cgggcatacc
     1921 tgaaccggac gccgaccgca tggccagctt cgccgcgcag attgccgccg tgaccgcgat
     1981 gaacgtgaag aacgtgccca gccgctgggg cgcgtcgatg gtcatcgtca tcggcatcgc
     2041 cggcgtggtc ggcgtgctga tcgcgctgct gtcgatggcg cgcggctttc aggccacgct
     2101 ggcgtccacc ggccgcgccg accgcgcggt gctgctgcgc ggcggcgcca acgacgagct
     2161 gtcgagcgtg atcatgcgcg agcaggtcga cgtggtgcgg caggcacccg gcgtgaagaa
     2221 ggatgcacag ggccagccga tggcgctgcc cgaggtgtac ctgctgtcgg ggctgccgcg
     2281 gcgcggcggc agcgagccgt ccaacgtggt ggtgcgcggc accgaggcgc gcgtgctcga
     2341 ggtgcggccg gagatccgca tcgtcgaagg ccgcatgttc acgccgggcc tgcgcgaggt
     2401 ggtggtcggg cgcggcgcgc aggcgcagtt cgccaacctc aatctgggcg accacgccgc
     2461 catccgcaac ggcgactggg cggtggtcgg catcttcgaa tccggcggcg acgtgcatga
     2521 atccgaggtg ctggccgacc tggagacgct gatgtcggcc gcgcaccgcc cggtgcccac
     2581 ctccatggtg gtgcagctgg aagacgccgc cgccttcacc cgcttcaagg acgcgctgac
     2641 cgccgacccg cgcatgtcgg tgaaggtcct gcgcgagccg gactactact ccagccggtc
     2701 ggaacagctg ggcgcgttca tcaactacct tgggtacacc gtcgcggtga tcatggcgat
     2761 cggcgcattg ttcggcgcgc tcaa
//
LOCUS       DASZZC010000146         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_846719,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000146 DASZZC010000000
VERSION     DASZZC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846719"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..236
                     /locus_tag="VHE37_03410"
     tRNA            161..236
                     /locus_tag="VHE37_03410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:194..196,aa:Arg,seq:cct)
     gene            547..623
                     /locus_tag="VHE37_03415"
     tRNA            547..623
                     /locus_tag="VHE37_03415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:581..583,aa:Arg,seq:tcg)
     gene            complement(626..1948)
                     /locus_tag="VHE37_03420"
     CDS             complement(626..1948)
                     /locus_tag="VHE37_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT34605.1"
                     /translation="MKFVWGALLLSSAALAAQEPLSFDQALAAAKARHQPVFVDFHAP
                     WCHSCFFMEKNVMNGADWDTVKLHAVIADVDADAGEGAAIAQQFKVAGYPTYIVLDES
                     GVELGRILGDRTRMQFYAELQPLLDRGATLERWQARVIDDGSDSLAAARIVFNAYEQR
                     MDAEHGLLWLASLPAPLQKAVAVDAVAGARINELKLLRANQQHDAAQCEAVAPAVIGG
                     ALDCDNVGFVSELRECIDPLPAEQKRAQLAPIVPRMAALQKQILVEHKGACSDTRGVV
                     DPAADLYDALDDKTAYRQVFEQGIAYSERGLKAGKGIDYRRDHYLADNLRYYLERLED
                     NARLDHVYPKLIAAYPEPYDYYYRYGKNLAKRGEYARALPLLEQAAPRSYGRNRLWVA
                     QWRAYTLLQLKRNDDANVVASEAMQANGPWFPDDIARLKGVLAGDKPK"
     gene            complement(1982..2058)
                     /locus_tag="VHE37_03425"
     tRNA            complement(1982..2058)
                     /locus_tag="VHE37_03425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2022..2024),aa:Pro,seq:tgg)
     gene            complement(2079..2154)
                     /locus_tag="VHE37_03430"
     tRNA            complement(2079..2154)
                     /locus_tag="VHE37_03430"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2119..2121),aa:Lys,seq:ttt)
     gene            complement(2201..2884)
                     /gene="queC"
                     /locus_tag="VHE37_03435"
     CDS             complement(2201..2884)
                     /gene="queC"
                     /locus_tag="VHE37_03435"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HVT34606.1"
                     /translation="MSTNEKAAVVLLSGGLDSATVLAMARAQGYACHALSVSYGQRHV
                     AELAAATRVAAALGAREHKQMHVNLDAVGGSALTDRAIDVPVGGLADGIPVTYVPARN
                     TLFLSLALGWAEVLGARDLFIGVNAVDYSGYPDCRPEFVRAFEALAALATKAGAEGAA
                     FRVHTPLISMSKADIIRAGTLLQVPYAETVSCYQADEAGRACGVCDSCRIRRDGFAAA
                     GVADPTRYR"
     gene            complement(2881..3567)
                     /gene="queE"
                     /locus_tag="VHE37_03440"
     CDS             complement(2881..3567)
                     /gene="queE"
                     /locus_tag="VHE37_03440"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HVT34607.1"
                     /translation="MREPSQQRDEAVAARAPRLKLTEIFLSLQGESSYAGWPTVFVRL
                     TGCPLRCQYCDTAYAFHGGQWHTLDDILAQVRAHGVRHVCVTGGEPLAQKQCIDLLRH
                     LCDAGFTVSLETSGAIDIAPVDARVARIVDIKTPGSGEQAKNRWENLALLTARDEVKF
                     VICSRADYEWAREVMRRERLAQRCSVLFSHSHGQLAPRELADWIVADRLPVRFQTQLH
                     KLLWGNVPGK"
     gene            complement(3700..>4028)
                     /gene="ybgF"
                     /locus_tag="VHE37_03445"
     CDS             complement(3700..>4028)
                     /gene="ybgF"
                     /locus_tag="VHE37_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HVT34608.1"
                     /translation="DEAIRGFRAVLDKWPNGRYAPNAAYWMGEASYVKRDYDGAQAAF
                     QMVLSKWPNDPRAPDALLKSGLVYNDMKQPDQARAQLQSVIAKYPNSNAAKLAQQKLA
                     SIPAKR"
BASE COUNT          658 a         1376 c         1289 g          705 t
ORIGIN      
        1 ttccctataa tcgcgccgcc tgacgcctgg ttcagtccgc ccggcggttt tcgcggcgcg
       61 ggttccgcta cgccggcgcc actctagccg agccgcggcg aaaaaatgtg cgctggaatt
      121 gggttttatc aattaggctg cgcacctctg gctggtcaac gccccggtag ctcagtggat
      181 agagcatctt cctcctaaga agagggtcgc aggttcgatt cctgctcggg gcgccaactt
      241 ggcttcttct ctgaccgtca ctccgcgggt cgcaggttgc gcggcgcgtc acgcaccgcg
      301 gcctacggcc tcgcgacggt cgctcaaatg cgcttcctgc ggatttgtcg attcctgctc
      361 ggggcgccaa ctcagtgatt gaagccgtcc tggctccgag ccagccgcgc atccgtgcgc
      421 ggcgttcgcc ggcgcgaatg tccaccggac attcgcaaca gacgccggtt caccctgcgg
      481 atttttcgat tcctgctcgg ggcgccaact caatatgcac cttcgccgct atgatcgcgt
      541 cttcgcgcgc ccgtagctca gttggataga gcatctggct tcgaaccaga gggccgggag
      601 ttcgagtctc tccgggcgcg ccaacctatt tcggcttgtc gccggccagc acgcccttga
      661 gccgcgcaat gtcatccggg aaccacgggc cgttggcctg catcgcctcg ctggcgacga
      721 cattcgcgtc atcgttgcgc ttcaactgca gcagcgtata ggcgcgccac tgcgccaccc
      781 acaggcgatt gcgcccatag ctgcgcggcg cagcctgctc cagcaacggc agcgcacggg
      841 catattcgcc gcgcttggcc aggttcttgc cgtagcggta gtagtagtcg tacggctcgg
      901 gatacgcggc gatcagcttc ggatagacat gatccagccg tgcgttgtcc tccaggcgct
      961 cgaggtaata gcgcaggttg tcggccaggt agtggtcgcg ccggtagtcg atacccttgc
     1021 cggccttgag cccgcgttcc gaataggcga tgccctgctc gaacacctgc cggtacgcag
     1081 tcttgtcgtc gagcgcgtca tagagatcgg ccgccggatc gaccacaccg cgcgtgtcgc
     1141 tgcacgcgcc cttgtgctcg accaggatct gcttctgcag cgcggccatg cgcggcacga
     1201 tcggcgccag ctgtgcgcgt ttctgctccg ccggcagcgg atcgatgcat tcgcgcagct
     1261 cgctgacaaa gccgacgttg tcgcaatcca gcgcgccgcc gatcactgcc ggcgccacgg
     1321 cctcgcattg cgccgcgtcg tgctgctggt tggcgcgcag cagcttgagc tcgttgatgc
     1381 gcgcgccagc cacggcatcc accgcgacgg ctttctgcag cggggccggc agcgaggcga
     1441 gccacagcaa gccatgctcg gcgtccatgc gctgctcgta ggcgttgaac acgatgcgcg
     1501 cggccgccag cgaatcactt ccatcatcga tcacgcgcgc ctgccagcgc tcgagcgtcg
     1561 cgccgcgatc gagcagcggc tgcagctcgg cgtagaactg catgcgcgtg cggtcgccaa
     1621 ggatgcggcc cagctccacg ccgctttcat ccagcacgat gtaggtggga tacccggcca
     1681 ccttgaactg ctgcgcgatg gccgcgccct cgcccgcgtc ggcatcgacg tcggcgatca
     1741 cggcatgcag tttcaccgtg tcccagtcgg cgccgttcat cacgttcttc tccatgaaga
     1801 agcacgagtg gcaccacggc gcgtggaaat cgacgaacac cggctggtgg cgcgccttcg
     1861 ccgcggccag agcctggtcg aatgacaagg gttcctgcgc cgccagcgcg gccgacgaca
     1921 gcagcagcgc accccagacg aatttcatgc gtgttcccaa aaatgccgcg atggcgggaa
     1981 atggtcggag cgagaggatt cgaacctccg accccttgcg cccaaggcaa gtgcgctacc
     2041 aggctgcgct acgctccgac gatgcatcca agtgtacttg gtgggtcgtg ctggattcga
     2101 accagcgacc aactggttaa aagccagctg ctctaccgac tgagctaacg acccctgttt
     2161 ttgggccgcg catgatagcg gaaaccgctc cacgcagaca tcagcgataa cgcgtgggat
     2221 cggccacgcc ggcggcggca aaaccgtcgc gccggatgcg gcaggaatcg catacgccgc
     2281 aggcgcggcc ggcttcatcg gcctggtagc acgacaccgt ctcggcgtat ggcacctgca
     2341 gcagcgtgcc ggcgcggatg atgtcggcct tgctcatgct gatcagcggc gtgtgcacgc
     2401 ggaaggccgc gccctcggcg ccggccttgg tcgccagcgc cgccagcgcc tcgaacgcgc
     2461 gcacgaactc cggccggcaa tccgggtagc cggaatagtc gacggcgttc acgccgatga
     2521 aaaggtcgcg cgcgcccagc acctcggccc agcccagcgc cagcgacagg aacagcgtat
     2581 tgcgtgccgg cacataagtg acgggaatgc catcggccaa tccgccaacc ggtacatcaa
     2641 tggcgcggtc ggtcagcgcc gagccgccga cggcatcgag attgacgtgc atctgcttgt
     2701 gctcgcgcgc gcccagtgcg gcggccacgc gcgtcgccgc ggcaagctcg gcgacatgcc
     2761 gctggccgta cgacaccgac agcgcgtggc acgcgtaacc ctgcgcgcgc gccatggcca
     2821 gcacggtggc ggaatcgagc ccgccggaga gcagcaccac cgcggctttt tcgttcgtgc
     2881 tcatttgccc ggcacattgc cccacagcag cttgtgcagc tgggtctgga agcgcaccgg
     2941 cagccggtcg gccacgatcc agtccgccag ttcgcgcggc gccagctggc cgtggctgtg
     3001 cgagaacagc acgctgcagc gctgcgccag tcgctcgcgc cgcatcactt cacgcgccca
     3061 ctcgtagtcg gcgcgcgagc agatgacgaa tttcacttcg tcgcgcgcgg tcagcagggc
     3121 aagattttcc cagcggttct tcgcctgctc gccggagccc ggcgtcttga tgtcgacgat
     3181 gcgcgccacg cgcgcatcca ccggcgcgat gtcgatggcg ccgctggttt ccagcgaaac
     3241 ggtaaagccg gcgtcgcaca ggtgccggag cagatcgatg cactgcttct gcgccagcgg
     3301 ctcgccgccg gtcacgcaca cgtggcgcac gccgtgagcg cgcacctgcg cgaggatgtc
     3361 gtcgagcgtg tgccactgcc cgccatgaaa ggcataggcg gtgtcgcagt actggcagcg
     3421 cagcgggcag ccggtgagcc gcacgaacac cgtgggccag ccggcatacg acgattcgcc
     3481 ctgcagcgag aggaaaattt ccgtgagctt gaggcgcggc gcgcgcgcgg cgaccgcttc
     3541 gtcgcgctgc tgcgacggct cccgcactgc cgttgtagat gccaccgccc ctctccgaat
     3601 gccgttatta attatctaac gaatttacat tcatcaaaca atttataacc acgcgaatgc
     3661 cggtgcggtg gaagcgaaaa cgcggcgcgc agccgcgctt cacctcttcg ccgggatgct
     3721 ggccagcttc tgctgcgcca gcttggcggc gttgctgttc ggatacttcg cgattaccga
     3781 ctgcaactgc gcgcgcgcct ggtcgggctg cttcatgtcg ttgtagacca ggccggactt
     3841 gagcagcgca tcgggtgcgc gcggatcgtt cggccacttg ctcagcacca tctggaacgc
     3901 ggcctgcgcg ccgtcgtagt cgcgcttgac gtagctggcc tcgcccatcc agtaggcggc
     3961 gttgggcgcg tagcggccgt tgggccactt gtcgagcacc gcgcggaaac cgcggatggc
     4021 ctcgtcgt
//
LOCUS       DASZZC010000147         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_779530,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000147 DASZZC010000000
VERSION     DASZZC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779530"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VHE37_03450"
     CDS             complement(<1..142)
                     /locus_tag="VHE37_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34609.1"
                     /translation="MTTNPLVTRLTLIASLGGLLFGYDTAVISGAVSSIDAYFIDPQH
                     LEE"
     gene            complement(392..1939)
                     /gene="treF"
                     /locus_tag="VHE37_03455"
     CDS             complement(392..1939)
                     /gene="treF"
                     /locus_tag="VHE37_03455"
                     /inference="COORDINATES: protein motif:HMM:NF009773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HVT34610.1"
                     /translation="MRPIMLAAGWLAAGCVLAQPVPSPSQQYGALYAQVETQSVFADS
                     KTFADAVPRAAPAAILRDYRAQHPATREQLRAFVRAHFILPDDGATAPATAGAPQPLA
                     QHIADLWPQLIRPPVAAAPGSSQLAFPYPHAVPGGRFREIYYWDSYFTMLGLQRDGHG
                     DVAQAMVNGFAGLIARYGHVPNGTRSYYLTRSQPPFFHRMVGLMTPQQPALAYQHYLA
                     ALRREHAWWMDGERGLRAGAAQRHVVRMADGALLNRYWDAGDTPRDESYREDIETARA
                     SGRPAAEVYRELRSAAESGWDFSSRWFADGKSLATIETTRIVPVDLNALLYGLERAIE
                     QGCAQARDLGCVHDYGARADARRVAMNQYLWDGGCGCYEDFQWRRGQRIERVTAATLY
                     PLFTGAADDAQAHAVAATARAQLLMPGGLATTRTATGQQWDAPNGWAPLQWIAIAGLR
                     AYGEDGLAGDIATRWLATVQAGYDGSGKLVEKYDVEQRRPGGGGEYPLQDGFGWTNGV
                     TRALLGP"
     gene            complement(2121..>2665)
                     /locus_tag="VHE37_03460"
     CDS             complement(2121..>2665)
                     /locus_tag="VHE37_03460"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVT34611.1"
                     /translation="GVVAGYLVLSAIAYAWHRTAHNVDVLWRGMHQLHHSPQRVDIPG
                     SVVFHPTEMVVQVLLQLFVTVIVLGLDPLAAALVGYVAAFYGMFQHWNVRTPAWIGYL
                     IQRPESHCVHHQRGLHRYNYADFPPWDILFGTFRNPRDWNGEAGFDAPAPRRIGAMLA
                     WQDVNLNLGAHTLGVTRMPR"
BASE COUNT          394 a          911 c          957 g          403 t
ORIGIN      
        1 tttcttccag gtgctgcgga tcgatgaagt aggcgtcgat cgacgacacc gcgccggaga
       61 tcaccgcggt gtcgtagccg aacagcaggc cgccgaggct ggcgatgagc gtgagccgcg
      121 tgaccagcgg gttggtagtc atgtgccgct ttctcctctt gtcgttataa acgccgctgc
      181 gcggcgcccc tcaccccgat cctttcccac gcatgaagcc gcgtggaggg agggaacaaa
      241 aaacctccct ctgcctgtgc gctgtttgca tgggaagagg gccggggtga ggggcgcgcg
      301 gcagcgcgcg ccggattcat cctataaaaa gaatgttcat tttatttgca ttctatgccg
      361 acatgccggg ttgcgcatct cgccgcgccg ctcacggccc cagcagcgcg cgcgtcacgc
      421 cgttggtcca gccgaagccg tcctgcagcg ggtactcgcc gccgccgccg ggccggcgct
      481 gctccacgtc gtacttctcc accagcttgc cgctgccgtc gtagccggcc tgcaccgtcg
      541 ccagccagcg cgtggcgatg tcgccggcga gtccatcctc gccgtaggca cgcaggccgg
      601 cgatggcgat ccattgcagc ggcgcccagc cgttgggcgc gtcccattgc tggccggtgg
      661 cggtgcgcgt ggtggccagg ccgccgggca tcagcagctg cgcgcgcgca gtggccgcca
      721 ccgcgtgcgc ctgcgcatcg tcggccgcgc cggtgaacag cgggtagagc gtggccgcgg
      781 tcacgcgttc gatacgctgg ccgcgccgcc actggaagtc ctcgtagcag ccgcagccgc
      841 cgtcccacag gtattggttc atcgccacgc ggcgcgcatc ggcgcgcgcg ccatagtcat
      901 gcacgcagcc caggtcgcgc gcctgcgcgc agccctgctc aatggcgcgc tccagcccgt
      961 acagcagcgc gttcaggtcc acaggcacga tgcgcgtggt ttcgatggtc gccagggatt
     1021 tgccgtcggc gaaccagcgc gaggaaaaat cccagccgct ttccgccgcg ctgcgcagct
     1081 cgcggtagac ctcggcggcg ggccggccgg atgcgcgcgc ggtctcgata tcttcccggt
     1141 acgactcgtc gcgcggggtg tcgccggcgt cccagtagcg gttgagcagc gcgccatcgg
     1201 ccatacgcac cacatgccgc tgcgccgcgc ccgcgcgcag gccgcgctcg ccgtccatcc
     1261 accaggcatg ctcgcgcctg agcgcggcga gatagtgctg gtaggccagc gccggctgct
     1321 gcggcgtcat caggccgacc atgcgatgaa agaacggcgg ctgcgagcgc gtgaggtagt
     1381 agctgcgtgt gccgttgggc acgtggccgt agcgcgcgat caggccggcg aagccgttga
     1441 ccatggcctg cgccacgtcg ccgtggccgt cgcgctgcag gccgagcatg gtgaagtacg
     1501 agtcccagta atagatctcg cggaagcgtc cgccgggcac cgcgtgcgga tatgggaaag
     1561 cgagctgcga cgagccgggc gccgcagcca ccggcggccg gatcagctgc ggccacaagt
     1621 ctgcgatgtg ctgcgccagc ggctggggtg cgccagcggt ggccggcgca gtggcgccgt
     1681 catcgggcag gatgaaatgc gcgcgcacga atgcgcgcag ctgttcgcgc gtggccggat
     1741 gctgcgcgcg gtagtcgcgc aggatggccg cgggcgcagc gcgcggcacg gcgtcggcga
     1801 aggtcttgct gtcggcgaag accgactgtg tctccacctg cgcgtagagc gcgccgtatt
     1861 gctgcgacgg cgacggcacc ggctgcgcca gcacgcagcc tgcggcaagc cagccggcgg
     1921 ccagcatgat cggtcgcatg gtgtccacct cctcctgcca tgggcgccta ccgtagccgg
     1981 atgccgcggc cgcgccccct cccgcaaagg aggggacatc ttcgcccatg acccgtctgc
     2041 aggaacatgt gacgggtttc gccccggttc cggcaatgga cgccgcgccg gcgcacgtcc
     2101 gtgcccgcgg caggccggcg tcagcgcggc atgcgcgtca cgcccagcgt gtgcgcgccg
     2161 aggttcaggt tgacgtcctg ccaggccagc atggcgccga tgcggcgcgg cgcgggggcg
     2221 tcgaagccgg cttcgccgtt ccagtcccgc gggttgcgga aggtgccgaa caggatgtcc
     2281 catggaggaa agtcggcgta gttgtaacgg tgcaggccgc gctggtgatg cacgcagtgc
     2341 gattcaggcc gctggatgag gtagccgatc caggccggcg tgcgcacgtt ccagtgctgg
     2401 aacatgccgt agaaggcggc gacgtagccc accagcgccg cggcgagcgg gtccaggccc
     2461 agcacgatca ccgtcacgaa gagctgcagc agcacctgca cgaccatctc cgtgggatgg
     2521 aagacgaccg agcccgggat gtcgacgcgc tgcgggctgt ggtggagctg gtgcatgccg
     2581 cgccacagga cgtccacgtt gtgcgccgtg cggtgccatg cgtaggcgat cgcgctcagc
     2641 acgaggtagc ccgcgacgac gccgc
//
LOCUS       DASZZC010000148         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_912307,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000148 DASZZC010000000
VERSION     DASZZC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912307"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VHE37_03465"
     CDS             complement(<1..290)
                     /locus_tag="VHE37_03465"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVT34612.1"
                     /translation="MSMDFITYAQNAEDVMLWRALREIERGFYIDVGAQDPIVDSVTK
                     AFYERGWHGINIEPVEHWYERLVHDRPHDINLRLAASDHSGEMNLFEIEATGL"
     gene            490..1902
                     /locus_tag="VHE37_03470"
     CDS             490..1902
                     /locus_tag="VHE37_03470"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HVT34613.1"
                     /translation="MLSPVILSGGSGTRLWPLSRKNLPKQFLALSGDATLFQQTVERT
                     RALAGVGAPIVVCSEEHRFLVAEQLRALNVEGASILLEPMPRNTAPAIALAACQALAN
                     DAEATLLVLPADHLIGDTSSFAEAVGKALPLAEQGWLVTFGIRPEAPETGFGYIKRAE
                     AAGAAGFRVERFVEKPDAATAQKYVEAGDYEWNSGMFLFKASRYLDELQQHAPTIYAA
                     GKAAFEKAKADLDFVRVDKEAFAASPDTSIDYAVMEKTTRAAVVPVSCAWSDIGSWDA
                     LWAASQRDGDGNRLEGDVIAIDSRNCFVRGTDRRLVAALGLDDIVIVDTPDAVLVAPR
                     TRVQDVKRLVERIKSDGRQEHMFHRKVYRPWGSYDSIDMGDRFQVKRITVKPGAALSL
                     QKHHHRAEHWVIVSGTAEVTRNDEVFLVAENESTFLPLGAVHRLRNPGKVPLELIEVQ
                     SGSYLGEDDIVRLEDVYGRA"
     gene            2020..>2422
                     /locus_tag="VHE37_03475"
     CDS             2020..>2422
                     /locus_tag="VHE37_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVT34614.1"
                     /translation="MSIPNRTRITDPLPRKLNLGCGFDYREGWLNVDLQSMHDPDLIA
                     DVTALPMLDGGAFEEILAQDVLEHFERAKTGVALAEWSRLLRPNGVLQLRVPSLFGMF
                     ELLARPDRRDPEQAEEVIHLMYGTQAYTGDYH"
BASE COUNT          483 a          753 c          749 g          437 t
ORIGIN      
        1 agaccggtgg cctcaatctc gaacaagttc atctcgccgg aatggtccga cgccgccaac
       61 cggagattga tgtcatgcgg ccgatcgtga acgagtcttt cgtaccaatg ctcgaccggc
      121 tcgatgttga tgccatgcca gccacgctca taaaaggcct tcgttactga atcgacaatc
      181 ggatcctggg ctcccacatc aatgtagaac cctcgctcaa tctcccgcag ggcacgccaa
      241 agcatcacat cttcggcatt ttgggcgtag gtaataaaat ccatggacat ggccgctgac
      301 ctgggaacgt gcaacaccgc tgcgggtaat acccacaaac ggactctcac cgcgggaaac
      361 cgcagcagac ccgtgcgttg attatcgccg aaaggcgccg cacctgtccc atccgcgccg
      421 ctcggcgcaa tggtcgtccg ccttgtgtga tgctaaattg ctcggccaac catcgaccgg
      481 attcacagca tgctctcccc cgtgatcctc agcggcggca gcggaacgcg cttgtggccg
      541 ctttcgcgca agaacctgcc caagcagttc ctggccctga gcggtgacgc cacgctgttc
      601 cagcagaccg tcgagcgtac ccgcgcgctg gccggcgtcg gggcgccgat cgtggtatgc
      661 agcgaggagc accgcttcct ggtcgccgag caattgcgcg cgctgaacgt cgagggcgct
      721 tcgatcctcc tggaaccgat gccacgtaat accgcgcccg cgattgcgct ggccgcgtgc
      781 caggcgctgg cgaatgacgc cgaagcaacg ctgctggtgc tgcctgccga tcacctgatc
      841 ggcgacactt ccagcttcgc cgaagccgtg ggcaaggcgc tgccgcttgc agaacaggga
      901 tggctggtga cgttcggcat tcgccccgaa gcacccgaga cgggtttcgg ctacatcaag
      961 cgcgccgaag cggccggtgc ggccggcttc cgcgtcgagc gcttcgtgga aaaacccgat
     1021 gccgcgacgg cacagaaata cgtcgaggcc ggcgactacg agtggaactc ggggatgttc
     1081 ctgttcaagg cgtcgcgcta tctcgacgag ctgcaacagc acgcgcccac gatttacgcg
     1141 gccggcaagg cggcgttcga aaaagcaaaa gccgatcttg atttcgtgcg cgtcgacaag
     1201 gaagcgttcg cggcttcgcc ggatacgtcc atcgactacg cggtgatgga aaagaccacc
     1261 cgcgcggccg tggtgccggt cagttgcgcg tggagcgaca tcggctcgtg ggatgcgctg
     1321 tgggcggcgt cgcaacgtga cggcgacggc aaccggctgg aaggcgacgt gatcgccatc
     1381 gatagccgca actgcttcgt gcgcggcacc gaccggcgcc tggtggcggc gctgggcctc
     1441 gatgatatcg tgatcgtgga tacgccggat gcggtgcttg tcgcgccgcg cacgcgcgtg
     1501 caggatgtca agcggctggt ggaacggatc aagtccgacg gacgccagga acacatgttc
     1561 caccgcaagg tataccgccc atggggcagc tacgactcca tcgacatggg cgaccgcttc
     1621 caggtcaagc gcatcacggt gaaaccgggt gccgcgctca gcctgcagaa gcatcatcac
     1681 cgcgccgaac actgggtgat cgtctcgggc acggccgaag tcacgcgcaa cgacgaagtg
     1741 ttcctggtgg cggagaacga aagcaccttc ctgccgctgg gcgcggtgca tcgcttgcgc
     1801 aatcccggca aggtgccgct ggaattgatt gaggtgcagt cgggcagcta cctgggcgag
     1861 gacgacatcg tgcggttgga ggatgtttac ggcagagcat gatgaggccg ctggatcgac
     1921 aagtccaacc ccattggagt gcgtgttcct ggcgaagtca tgaggccttg ccgccacacg
     1981 ccgacgcaac ttcagatctg atccagccga ggccagccaa tgagcattcc gaaccgcacc
     2041 aggatcactg atccgcttcc tcgcaagctc aatctcggct gtggatttga ttaccgggag
     2101 ggctggctca acgtcgatct gcaatcaatg catgatccag acctcatcgc ggatgtcact
     2161 gcgcttccga tgctggatgg cggcgcgttc gaagaaatac tggctcaaga cgttctcgaa
     2221 catttcgagc gtgcaaaaac cggcgtggcg cttgctgaat ggtcgcgact tttgagaccc
     2281 aatggcgtat tgcaacttcg cgtgccgagc ctcttcggga tgtttgaact acttgcacga
     2341 cccgaccgtc gggatcccga acaggccgag gaggtcatcc atctcatgta tgggactcaa
     2401 gcctacacgg gcgactacca tc
//
LOCUS       DASZZC010000149         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_912378,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000149 DASZZC010000000
VERSION     DASZZC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912378"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..2059)
                     /locus_tag="VHE37_03480"
     CDS             complement(320..2059)
                     /locus_tag="VHE37_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HVT34615.1"
                     /translation="MCLLLASGEVFAAGDAAQPANPLPANPGVQLMSSQSLSDPSNVP
                     PTLRAPEPAPLELPAARPVARASTLPPWDESAFPHYPLLRPAVSFWTDVFGDYSELQS
                     VIHSENDPSKVFKVLDFRADAERLDDFALARKRANEENRAKDYANLLLKQVHELRHTP
                     EKMNAEQRYVYNLYDDQKADDLRFKKAIGTLRAQRGLKERTEKALDTASGYLPQMESI
                     FKKHDLPTQLTRLPLVESSFNVEAYSKVGAAGLWQFMPSSARIYMRLDEVVDDRRDPW
                     TSTEGAAGHLADDYAELHSWPLALTAYNHGRAGIARGLAQTHGTDLTDLIERYRNKNF
                     GFASKNFYAEFLAASAVERRFRESQANAKARQVLAFDTVETKHYVPYETLRKLCGADD
                     EVFRKLNPAYRPEVIEGKLYVPPGHIIRVPAGTAKTFEVAYEKLGSNERFDEQKVYYI
                     LHKVGRGDSLGRIARKYGVAQRAILDANGLKDGKRLKVGTVLRVPPRTEKRLAPITVA
                     VGETEPAQTRDQKVAEAVEANYRVHKVQLGQTLGSIAKRYKVSVASLRAANNLGNSSH
                     IRPGQKLKVPANS"
     gene            2346..3293
                     /gene="pip"
                     /locus_tag="VHE37_03485"
     CDS             2346..3293
                     /gene="pip"
                     /locus_tag="VHE37_03485"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HVT34616.1"
                     /translation="MAHPYPPIEPYRTHRLRVSALHEIYVEECGNPAGRPVVFVHGGP
                     GGGTEPWQRTFFNPQKYRIVLFDQRGCGKSTPHAELAENTTWDLVADMEKIRALLNIR
                     KWVVFGGSWGSTLALAYAQTHPGAVTGLILRGIFLLRKAEIDWFYQEGANWIFPDAWE
                     AYRDHIPPAERGDFVSAYHRRLTSSDAAVRLAAARAWSTWEGATSKLFPSEDMVSRYA
                     ADQFADAFARIECHYFVNRGFFRCDDQLLVDAQKIRHIPGVIIQGRYDVVCPPRSAWD
                     LHRAWPEAQLQIVGDAGHAASEPGIYSRLIDAADRYADL"
     gene            complement(3307..3567)
                     /locus_tag="VHE37_03490"
     CDS             complement(3307..3567)
                     /locus_tag="VHE37_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34617.1"
                     /translation="MRAELERRCVSLILPVVAAYVPGLLLCMLALRKALHLLPPELVA
                     PATVLILMMLWGWMQLCSRLLPPERPQPVRNVRLAPIPVRRF"
     gene            complement(3694..4176)
                     /locus_tag="VHE37_03495"
     CDS             complement(3694..4176)
                     /locus_tag="VHE37_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVT34618.1"
                     /translation="MSREKLRLRLERTDARILRELQKDGRLPIVELAAKVSLSPTACL
                     RRVRKLEESGVIERYAAVLSPAALGQDIQAFISVSIERQSKDVAEAFEAAIQKRPEVR
                     ACYVMTGDLDFLLHVFVADLQAFAEFSMKVLIGLPGVKDVRSSLVLEAVKKDEGIALA
                     "
     gene            4286..>4530
                     /locus_tag="VHE37_03500"
     CDS             4286..>4530
                     /locus_tag="VHE37_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34619.1"
                     /translation="MHVSRYVSRTPDVAGLVRYTDDENRVWAELYARQMRVIDGRACN
                     EFIDGLRLLALPHDRVAQLPDVSAVLRQATGWEVVAVP"
BASE COUNT          733 a         1505 c         1458 g          834 t
ORIGIN      
        1 tgctgatcgg gccgtttctg ggcgccgcgc tcggcgaact cggcgagcgg cgctcgctgc
       61 agcaggcggc ggcgtcgggc atcggcacgc tgatcggcct gctgctgggc acggtgatga
      121 aggccgcgct ggccttcctc atgctgggcg tgttcgccct gccgttcctg ctttaaggaa
      181 gctctgacaa agctgctgcg ctcggcagat tgcgcgaggg cagtgctcgg aatcctcaca
      241 tactcccttc gtatgctgcg gttcctgcgc gctgtccccg cgcaatctgc cgtcgctcgc
      301 gacgcttttt ccgagcttcc taggagttcg ccggcacctt gagcttctgt cccggccgga
      361 tatggctgga gttgcccagg ttgttggccg cgcgcagcga cgccacgctg accttgtagc
      421 gcttggcgat gctgcccagc gtctggccca gctgcacctt gtgcacgcgg tagttggctt
      481 ccacagcctc cgcgaccttc tggtcgcgcg tctgcgccgg ctcggtttcg ccgacggcca
      541 ccgtgatcgg cgccagccgc ttctcggtgc gcggcggcac gcgcagcacc gtaccaacct
      601 tgagccgctt gccgtccttc aggccgttgg cgtcgaggat cgcgcgctgc gccacgccgt
      661 acttgcgcgc gatcctgccg agcgaatcgc cgcgcccgac cttgtgcagg atgtagtaca
      721 ccttctgttc gtcgaagcgc tcgttgctgc ccagcttctc gtaggccact tcgaaggttt
      781 tcgcggtgcc ggccggcacg cggatgatgt ggcccggcgg cacgtagagc ttgccttcga
      841 tcacttccgg ccggtacgcg ggattgagct tgcggaacac ctcgtcgtcg gcaccgcaca
      901 gcttgcgcag ggtttcatac ggcacgtagt gcttggtctc caccgtgtcg aaggccagca
      961 cctgcctggc tttcgcgttg gcctggcttt cgcggaagcg ccgctccacc gcgctggcgg
     1021 ccaggaactc cgcgtagaaa ttcttcgagg cgaaaccgaa gttcttgttg cggtagcgct
     1081 cgatcaggtc ggtgagatcg gtgccgtggg tctgcgccag cccgcgcgcg atgccggcgc
     1141 ggccgtggtt gtacgcggtg agcgccagcg gccagctgtg cagctcggcg tagtcgtcgg
     1201 ccaggtgccc ggcggcgccc tcggtggagg tccacgggtc gcggcggtcg tcgaccactt
     1261 catccagccg catgtagatg cgcgccgacg acggcatgaa ctgccacagg ccggcggcgc
     1321 cgaccttgga ataggcctcg acgttgaacg acgattccac cagcggcagg cgcgtgagct
     1381 gcgtcggcag gtcatgcttc ttgaagatgc tttccatctg cggcaggtag ccgctggcgg
     1441 tgtcgagcgc cttctcggtg cgctccttca gtccgcgctg tgcgcgcagc gtgccgatgg
     1501 ccttcttgaa gcgcaggtcg tcggctttct ggtcgtcgta caggttgtac acgtagcgct
     1561 gctcggcatt catcttttcc ggcgtgtggc gcaactcgtg cacctgcttg agcagcaggt
     1621 tggcgtaatc cttcgcgcgg ttttcctcgt tggcgcgctt gcgcgccagc gcgaaatcgt
     1681 cgagccgctc ggcgtcggcg cggaagtcca gcaccttgaa caccttgctt ggatcgttct
     1741 ccgaatggat cacgctctgc agttcggagt aatcgccgaa cacatcggtc cagaacgaca
     1801 ccgccggcct gagcagcggg taatgcggga aggcggactc gtcccacggc ggcagcgtgc
     1861 tggcgcgcgc aaccggccgg gccgcaggta actcgagcgg cgcgggttcg ggcgcgcgca
     1921 gcgtgggcgg cacgttggag ggatcgctca gcgattgcga cgacatcagc tgcacgccag
     1981 gattggccgg cagcggattg gccggctggg ccgcatcgcc ggcggcgaac acctcgccgc
     2041 tggccagcag caggcacatc aacacacgag cgtatcggtc catccgttcc atcagtccaa
     2101 tcgagaaatc gtgcgcagca aaaacatccg cgcatgcatg cgatggtaac aggcggattc
     2161 gcgcatgcga ctggattttt tgacgccacc gcagtgcaga ttgcactgtt attaattaat
     2221 gcatgaaatt taattaatta tttatattat aacacggtaa agggcggtgc ggtgaggccg
     2281 gaaatgcgcg ctaacacttc gcatacactg cgccgatccc tgcacgccga cctgaccccc
     2341 gcaccatggc ccacccgtat ccgcccatcg agccgtaccg cacgcaccgc ctgcgcgtgt
     2401 ccgcgctgca cgagatctac gtggaggaat gcggcaaccc cgccggcagg ccggtggtgt
     2461 tcgtgcatgg cggccccggc ggcggcaccg agccgtggca gcgcaccttc ttcaacccgc
     2521 agaaataccg catcgtgctg ttcgaccagc gtggctgcgg caagtcgaca ccgcacgcgg
     2581 aactggcgga gaacaccacc tgggacctgg tggccgacat ggaaaaaatc cgcgcgctgc
     2641 tgaacatccg caagtgggtg gtgttcggcg gctcctgggg cagcacgctg gcgctggcct
     2701 acgcgcagac gcatccgggc gcggtcaccg gcctgatcct gcgcggcatc ttcctgctgc
     2761 gcaaggcgga gatcgactgg ttctaccagg aaggcgccaa ctggattttc ccggatgcct
     2821 gggaggccta ccgcgatcac atcccgccgg cggagcgcgg cgatttcgtc agtgcctacc
     2881 accgccgcct gaccagcagc gacgccgcgg tgcgcctggc cgcggcgcgc gcgtggagca
     2941 cctgggaggg cgccaccagc aagctgtttc cgagcgagga catggtgtcg cgctatgccg
     3001 ccgaccagtt cgccgatgcc tttgcgcgca tcgaatgcca ctacttcgtc aaccgcggct
     3061 tcttccgctg cgacgaccag ctgctggtgg atgcgcagaa aatccgccac atcccgggcg
     3121 tgatcatcca gggacgctac gacgtggtgt gtccgccgcg ctcggcctgg gacctgcacc
     3181 gcgcctggcc cgaagcccaa ctgcagatcg tgggcgacgc cggccacgcc gccagcgaac
     3241 cgggcatcta ttcgcggctg atcgacgccg cggatcgcta cgccgatctg taacggcgaa
     3301 tttccttcag aagcggcgca ccgggatcgg cgccagccgc acgttgcgca ccggctgcgg
     3361 acgctcgggc ggcagcaggc ggctgcacag ctgcatccag ccccacagca tcatcaggat
     3421 cagcacggtt gccggcgcca ccagctcggg cggcagcagg tgcagcgcct tgcgcagcgc
     3481 cagcatgcac agcagcaggc ccggcacata ggccgccacc accggcagga tcaggctcac
     3541 gcaacgtcgt tcgagttcgg cacgcatgga cttttccgct tcgctttcgt acatccgcat
     3601 ggcaaccagt atccttctcc aaggaactgg accatttcag cgcgtagtgg ttcgaagcgt
     3661 taccctgcgt tacatactgg tgagtaccgc tcatcacgcc agcgctatcc cttcgtcctt
     3721 tttcaccgct tccagcacca ggctggagcg cacgtccttc actcccggca ggccgatcag
     3781 taccttcatc gagaactcgg cgaaggcctg caggtcggcc acgaacacgt gcagcaggaa
     3841 atccaggtcg ccggtcatca catagcaggc gcgcacctcc ggccgcttct ggattgccgc
     3901 ctcgaacgcc tcggccacgt ccttggactg ccgctcgatg ctgacgctga tgaaggcctg
     3961 gatgtcctgg cccagcgcgg ccggcgacag caccgcggcg tagcgctcga tcacgccgct
     4021 ctcctccagc ttgcgcacgc ggcgcaggca ggcggtgggc gacagcgaca ccttggccgc
     4081 cagttcgacg atgggcagcc gcccgtcctt ctgcagctcg cgcaggatgc gggcatcggt
     4141 gcgttccagc cgcagtcgca gtttttctct actcatgtcg aataaattag tgaatacctg
     4201 cgaaatattg cggtctaaag cagcaaatag caaagaaacg gcgcacgcag cgcgataaag
     4261 tgtgttcctg ggtggaggac cgatcatgca tgtgagccgc tatgtttcgc gcacgccgga
     4321 tgttgccgga ctggtgcgct ataccgacga cgaaaaccgc gtgtgggccg agctgtacgc
     4381 gcggcagatg cgcgtgatcg atggccgcgc gtgcaacgaa ttcatcgacg gcctcaggct
     4441 gctggcgctg ccgcatgacc gcgtggcgca gctgcctgac gtgtccgccg tgctgcgcca
     4501 ggccaccggc tgggaagtgg tggccgtacc
//
LOCUS       DASZZC010000150         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_525245,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000150 DASZZC010000000
VERSION     DASZZC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525245"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VHE37_03505"
     CDS             <1..943
                     /locus_tag="VHE37_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HVT34620.1"
                     /translation="DTPVSLPKIRAADVHLHYTGAQILGRSMPLDNIVAQLDIVDGRI
                     AVHPLTFQVGKGSIALDLAADAQQDLVHAAGDVDFRQVDLAHLMQKMTVFKGAGTIGG
                     KARFESTGNSVASLLGHGNGEMQLFTAGGDLSALLVDLAGLDFGGALMSAIGLPSKAA
                     LRCAIADYSLDKGVLNTRLLLLDTTEANITGSGKVNLDQETVDYQLKTEPKHLSVLIL
                     KGPIDISGTLRNPKIRPDAAGIAKRAVPAAILGVLLTPLAALLPTITLGLGEDNNCTR
                     LVHEAQAQAAQTGAQGKAAVQKNAGDAAKKPVTAAPK"
     gene            complement(1028..1369)
                     /locus_tag="VHE37_03510"
     CDS             complement(1028..1369)
                     /locus_tag="VHE37_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT34621.1"
                     /translation="MHRKQLRFGRGFRVALSNARAQAAEMTLAPGASEGGAHNAHRGA
                     DQWLFVVEGEGVVRKDGRRFPLRAGTLLLIEAGDHHEVLNTGEAPLRTLNFYAPPAYD
                     ARGEPLPRGRR"
     gene            complement(1380..>2006)
                     /locus_tag="VHE37_03515"
     CDS             complement(1380..>2006)
                     /locus_tag="VHE37_03515"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVT34622.1"
                     /translation="ARSGAMSPPYLRRKPRGQRLYSRATEAPMHDGPSREESLQQLRP
                     LIEDIAIATLTTIDEDGGLRSRPMATQSIDDDGVLWFFTNDYAPKVAEVLLHPEVCVT
                     YADPARQRYVSVSGKAELVREHEEMKRRWQPELQAWFPHGLEEQDLGLLRIEIEHAQY
                     WDAGESRLVQLFARARAALTGAPPPAHAGNGTAHKLTLKTRIAPDNIS"
BASE COUNT          314 a          684 c          690 g          318 t
ORIGIN      
        1 ggacacgccg gtatcgctgc ccaagatccg cgccgccgac gtgcacctgc actacaccgg
       61 cgcgcagatc ctcggccgca gcatgccgct ggacaacatc gtggcgcagc tggacatcgt
      121 cgatggccgc atcgcggtgc acccgctgac cttccaggtg ggcaagggca gcatcgcgct
      181 ggatctcgcc gccgatgcgc agcaggacct ggtgcatgcc gccggcgatg tcgacttccg
      241 ccaggtggac ctcgcccacc tgatgcagaa gatgaccgtc ttcaagggcg ccggcaccat
      301 cggcggcaag gcgcgcttcg aatccaccgg caactcggtg gccagcctgc ttggccatgg
      361 caatggcgag atgcagctgt tcaccgccgg cggcgacctg tcggcgctgc tggtcgacct
      421 ggccggcctg gacttcggcg gtgcgctgat gtcggccatc gggctgccgt cgaaggcggc
      481 gctgcgctgc gccatcgccg actactcgct ggataaaggc gtgctcaaca cgcggctgct
      541 gctgctcgac accaccgagg ccaacatcac cggcagcggc aaggtgaacc tcgaccagga
      601 aacggtcgac taccagctca agaccgagcc caagcacctg tcggtgctga tcctcaaggg
      661 gccgatcgac atcagcggca cactgcgcaa tccgaagatc cggccggacg ccgcgggcat
      721 cgccaagcgc gcggtgccgg cggcgatcct cggcgtgctg ctgacgccgc tggccgcgct
      781 gctgccgacc atcacgctcg gcctgggcga ggacaacaac tgcacgcgtc tggtccacga
      841 ggcgcaggcg caggctgcgc agaccggcgc gcagggcaag gctgcggtgc agaaaaatgc
      901 cggcgacgcg gcgaaaaaac cggtgaccgc agcgccaaaa tgagtgttat taataattgt
      961 ctatttttat ttttagacga ttatctataa catgcctgga gcgggtgcgg tggatgaaaa
     1021 aattggccta tcgacggcca cgcggcagcg gttcgccgcg cgcgtcgtaa gccggcggcg
     1081 cgtagaagtt gagcgtgcgc agcggcgcct cgccggtgtt gagcacctcg tggtgatcgc
     1141 cggcctcgat cagcagcagc gtgccggcgc gcagcgggaa gcggcggccg tccttgcgca
     1201 ccacgccctc gccttccacc acgaacagcc actggtccgc gccgcggtgc gcgttgtgcg
     1261 cgccgccctc ggaagcgccg ggagcgaggg tcatctccgc ggcctgcgcg cgtgcattgg
     1321 acagcgccac gcggaagccg cggccgaagc gcagctgctt gcggtgcacg cgtgcggcct
     1381 cagctgatgt tgtcgggggc gatgcgcgtc ttcagcgtga gcttgtgcgc cgtgccgttg
     1441 ccggcatgcg ccggcggcgg cgcgccggtg agcgcggcgc gcgcgcgcgc gaacagctgc
     1501 accagcctgc tctcgccggc atcccagtac tgcgcatgct caatctcgat gcgcagcagg
     1561 ccgagatcct gttcctccag accgtgcggg aaccaggcct gcagctccgg ctgccagcgc
     1621 cgcttcatct cctcgtgctc gcgcaccagt tcggccttgc ccgacaccga gacgtagcgc
     1681 tggcgcgccg ggtcggcata ggtcacgcac acctcgggat gcagcagcac ttcggccacc
     1741 ttgggcgcgt agtcgttggt gaagaaccac agcacgccgt catcgtcgat ggattgcgtg
     1801 gccatcggcc ggctgcgcag gccgccgtcc tcgtcgatgg tggtcagcgt ggcgatggcg
     1861 atgtcctcga tcagcggccg cagctgctgc aggctttcct cgcgggatgg tccgtcgtgc
     1921 atgggggcct cggtggctcg gctgtacagg cgctggccgc gcggcttgcg gcgcaggtag
     1981 ggtgggctca tcgctccgct ccgcgc
//
LOCUS       DASZZC010000151         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_589850,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000151 DASZZC010000000
VERSION     DASZZC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589850"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VHE37_03520"
     CDS             complement(<1..1678)
                     /locus_tag="VHE37_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT34623.1"
                     /translation="MSGRSLRGRVGPALAAAAMVFASAQAAPPATTVEGHLLAGHVTL
                     DGKALPGASVVLTSGGGGTFVRTDDSGAYAFRDLLDGDYTISVRLPGYGFTPPRRTLH
                     LQDFDASSLDFDAVYIGAPPGSGIVVAPPPSTMPPAVAPAAPSAAAASVPAVPSAPPP
                     AAAPPVQTPAPAYPSAPAPSITAAAPAAEALPAASAPAAAASASAPAPATPAASAAVS
                     APVAPIATPPPAPTAAAAEVTGHAYAKHGTGMGGVTVGYNMTRRPAVDARLPAVITGE
                     DGSFTLPALPPHPAYEPQPWIEAHGPLFQYCPPQVFLPAPNPVEFRACDPPEPSKAPH
                     QLSGSVTRLGKPLPRVEIHQAFSSRRGYTYEEGPLGPVVAYSDENGHWQFTAPSVSAR
                     NEVSGYFSYLVAVLPDGPRFCPGQLFLPEGAGNLDHLDFAACGAEGSLAVGSSRIAAA
                     PASGSIQDVALALVHGDIAVAWVQDVGAISTVWFARWNGKSWSAPVAAANGHVADDPN
                     HRLSLAAAGDHLALAYLDRDDALGTHVAVSLSDDGGHGWHGEPVATFVAHS"
     gene            1802..>1929
                     /locus_tag="VHE37_03525"
     CDS             1802..>1929
                     /locus_tag="VHE37_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HVT34624.1"
                     /translation="MSLRLGDIAPDFTADTTQGQIHFHQWLGNSWGVLFSHPRDYTP"
BASE COUNT          295 a          663 c          704 g          267 t
ORIGIN      
        1 aactgtgcgc cacgaaggtg gccaccggct cgccgtgcca accgtggccg ccgtcatcgg
       61 acagcgacac cgccacgtgc gtgcccagcg catcgtcgcg gtcgagatag gccagcgcga
      121 gatgatcgcc ggcggcggcc agcgacaggc ggtgattcgg atcatcggcg acatggccgt
      181 tggccgcggc caccggcgcg ctccagctct tgccgttcca gcgcgcgaac cacacggtgg
      241 agatggcgcc tacgtcctgc acccaggcca ccgcgatgtc gccgtggacc agtgccagcg
      301 ccacgtcctg gatgctgccg gacgccggcg ccgccgcgat gcggctgctg cccactgcca
      361 ggctgccctc cgcgccgcag gcggcgaagt ccaggtggtc gagattgccg gcgccctcgg
      421 gcaggaacag ctggccgggg cagaagcgcg ggccgtcggg cagcaccgcc accaggtagg
      481 agaaatatcc ggagacttcg ttgcgcgccg acaccgacgg cgcagtgaac tgccagtggc
      541 cgttctcgtc gctgtacgcc accaccgggc ccagcggccc ttcctcgtag gtgtagcccc
      601 tgcgcgagga gaacgcctgg tggatttcaa cgcgcggcag cggcttgccc aggcgcgtca
      661 cgctgcccga cagctggtgc ggcgcctttg acggctccgg cggatcgcag gcgcggaact
      721 cgacaggatt gggcgccggc aggaagacct gcggcgggca gtactggaac agggggccat
      781 gcgcctcgat ccagggctgc ggttcgtaag ccgggtgcgg tggcagcgcg ggcagggtga
      841 agctgccgtc ctcgccggtg atgaccgcgg gcaggcgcgc atccacggct ggccggcgcg
      901 tcatgttgta gccgacggtg acgccgccca tgccggtgcc gtgcttggcg taggcgtgcc
      961 cggtgacttc ggccgccgca gccgtcgggg cgggcggcgg cgtggcgatg ggggcaaccg
     1021 gcgctgaaac ggcggcgctt gccgcaggcg tggctggtgc cggtgcagat gcagatgctg
     1081 cagcggcggg cgcgctcgcg gcaggcagtg cttcggcagc cggcgctgca gccgttatcg
     1141 aaggagccgg tgcagaagga taggccggtg ctggtgtctg aaccggtggc gcggctgccg
     1201 gcggcggtgc gctcgggacc gccggaaccg acgcagccgc ggccgatggc gcggcaggag
     1261 caaccgccgg aggcatcgtc gagggtggcg gtgccaccac gatgccgctg cccggcggag
     1321 caccgatgta gacggcgtcg aagtccagcg aactggcgtc gaaatcctgc aggtgcagtg
     1381 tgcgccgcgg cggcgtgaag ccgtagccgg ggaggcgcac gctgatggtg tagtcgccgt
     1441 cgagcaggtc gcggaaggca tacgcgccgc tgtcatcggt gcgcacgaaa gtgccgccgc
     1501 cgccgctggt gagcaccacg gacgcgccgg gcagcgcctt gccgtcgagc gtcacgtgac
     1561 ccgcaagcag gtgaccttcc acggtggtcg ccggcggtgc ggcctgcgcc gaagcgaaca
     1621 ccatcgctgc cgccgccagt gccggtccca cccggccgcg caaactccgt cccgacattc
     1681 ctcgctcctc gattctttct aatgccgcgc tcgaataatg atatgaaatc ggccgggacc
     1741 gagcttgcgc gggggccgca atggttcatc attgccaccc cccaccgcaa ggagaagctc
     1801 catgagcctg cgactcggcg acattgcccc cgatttcacc gcggacacca cccagggcca
     1861 gatccatttc caccaatggc tcggcaacag ctggggcgtg ctgttcagcc acccgcgcga
     1921 ctacacccc
//
LOCUS       DASZZC010000152         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_653828,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000152 DASZZC010000000
VERSION     DASZZC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653828"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /gene="uvrB"
                     /locus_tag="VHE37_03530"
     CDS             <1..1538
                     /gene="uvrB"
                     /locus_tag="VHE37_03530"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HVT34625.1"
                     /translation="FKMVLHLDKGARMSQRDIIHTLTAMQYTRNDAALERGTYRVRGD
                     VIDIHPAESDEEAVRVELFDDEIETLLYFDPLTGEVRGKVPRLTVYPKTHYVTPREKT
                     LAMLDQIRGDLQLRLEELRKLNKLVEMQRLDQRTHFDLEMIQEIGYCNGIENYSRYLS
                     GRLPGQPPPCLFDYLPPDALVVVDESHVTVPQLGAMYKGDRSRKETLVEYGFRLPSAL
                     DNRPLRFDEWEAICPQIIFVSATPGPYEAQKSGQVAEQLVRPTGLVDPEVEIRPASSQ
                     VDDLLGEVRIRADKKERVLVTVLTKRMAEDLTDYFREHGVKVRYMHSDIDTVERAEII
                     RDLRLGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARHLG
                     GKAILYADEITGSMRRALDETDRRRAKQEAYNQEHGITPRGVKKRIADVMRYGYDDGV
                     APLKAAEPQNEYASLPPEKLTRVISKLEKEMRDAAQNLEFELAAKKRDELRKLRELNL
                     RNR"
     gene            1754..>2151
                     /locus_tag="VHE37_03535"
     CDS             1754..>2151
                     /locus_tag="VHE37_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34626.1"
                     /translation="MPEQSVGETAGETAGGDLAAVAEAADEAMQPAATAVEAQFRRAL
                     ALMRTDAEGAGVEALRGTPWCASSRGEVIHYHYRCASLDPEFRDFVRRADAARDLANF
                     NEAEYFYYRALQLYPLHGGYMVQYAHMLKD"
BASE COUNT          419 a          725 c          695 g          312 t
ORIGIN      
        1 acttcaagat ggtgctgcac ctggacaagg gcgcgcgcat gagccagcgc gacatcatcc
       61 acacgctcac cgccatgcag tacacgcgca acgacgcggc gctggagcgc ggcacctacc
      121 gggttcgcgg cgacgtcatc gacatccacc cggccgaatc cgacgaggaa gcggtgcgcg
      181 tggagctgtt cgatgacgag atcgagacgc tgctgtactt cgacccgctc accggcgagg
      241 tgcgcggcaa ggtgccgcgg ctcaccgtgt acccgaagac gcattacgtg acgccgcgcg
      301 agaagacgct ggccatgctc gaccagatcc gcggcgacct gcagctgcgc ctggaagaac
      361 tgcgcaagct gaacaagctg gtggagatgc agcggctgga ccagcgcacg cacttcgacc
      421 tggaaatgat ccaggagatc ggctactgca acggcatcga gaactactcg cgctacctgt
      481 ccggccgtct gcccggccag ccgccgccct gcctgttcga ttacctgccg cccgacgcgc
      541 tggtcgtggt cgacgagtcg cacgtcaccg tgccgcagct gggcgccatg tacaagggcg
      601 accgctcgcg taaggagacg ctggtggaat acggtttccg gttgcccagc gcgctcgaca
      661 accgtccgct gcgcttcgac gagtgggaag cgatctgccc gcagatcatt ttcgtctccg
      721 ccacgcccgg cccgtacgag gcgcagaaat ccggccaggt ggccgaacag ctggtgcgcc
      781 cgaccgggct ggtcgatccg gaagtggaaa tccgcccggc ctcgtcgcag gtcgacgacc
      841 tgctcggcga agtgcgcatc cgcgccgaca aaaaggagcg cgtgctggtc accgtgctga
      901 ccaagcgcat ggctgaagac ctcaccgatt acttccgcga gcacggcgtg aaagtgcgct
      961 acatgcacag cgacatcgac acggtggagc gcgccgagat catccgcgac ctgcggctgg
     1021 gcgagttcga cgtgctggtc ggcatcaacc tgctgcgcga aggtctcgat ctgccggaag
     1081 tctcgctggt ggccatcctc gacgccgaca aggaaggctt cctgcgcagc gagcgctcgc
     1141 tgatccagac catcggccgc gccgcgcgcc acctcggagg caaggcgatc ctctatgccg
     1201 acgagatcac cggctcgatg cgccgtgcgc tcgacgagac cgaccgccgc cgcgccaagc
     1261 aggaggcgta caaccaggag cacggcatca cacccagggg cgtgaagaag cgcattgccg
     1321 acgtcatgcg ctacggctac gacgacggcg tggcgccgct gaaggcggcc gagccgcaga
     1381 acgaatacgc ctcgctgccg ccggagaagc tgacccgcgt catttccaag ctggaaaaag
     1441 agatgcgcga cgccgcgcaa aacctggaat tcgaactggc ggcgaagaag cgcgacgagc
     1501 tgcgcaagct gcgggagctc aacctgcgca accgctgatg gcgcaacaat ttgtgctggt
     1561 gcacaaagtc ccgttgacta cggtcttgcc ccgcatatag tgtgccgctc ctgccgcccc
     1621 tcggcggcga ccagatcccg cggtctcgca gaagatcacc gggcatcgca gggaatgtgg
     1681 aatgccaacc gtcagtgaac tgaaactgca acgcgatgcc gcggacgaac ccggcttggc
     1741 ggatgccgtg cccgtgccag aacaatccgt gggcgaaacc gccggtgaaa cggccggcgg
     1801 tgacctcgcc gcagtcgccg aagcggcaga tgaagcaatg cagccggcgg cgacggcagt
     1861 ggaggcgcaa ttccgccgtg cactcgcgtt gatgcgtacc gatgccgagg gcgccggcgt
     1921 ggaagcgctg cgcggcacgc cctggtgcgc atccagccgc ggcgaggtga tccattacca
     1981 ctaccgctgc gcctcgctcg acccggaatt ccgcgacttc gtccgccgcg ccgacgccgc
     2041 ccgcgacctg gccaacttca acgaggcgga atacttctat taccgcgcgc tgcagctgta
     2101 cccgctccac ggtggttaca tggtgcagta cgcgcacatg ctgaaggacc a
//
LOCUS       DASZZC010000153         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_653832,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000153 DASZZC010000000
VERSION     DASZZC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653832"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VHE37_03540"
     CDS             <1..849
                     /locus_tag="VHE37_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34627.1"
                     /translation="RPRYSSGAQGSYALGLVHERYRGADTIHHAGATLGCVHQLLSVP
                     AHGLDLSIISNRMDGAAPGLAWKVVDAVLGDVLAPAVPPLAPAEHEAVTGRWYAPASR
                     RLYTVMQQPQPNQSATLALAVNDGPIGLLRQKDGGYFADSPAHGTVDLRVPAGAGARP
                     ASLELSDSGHVERVQRLPDSGPDATALGAELVGRYRYADFGIELAVIVESGKLFLDLR
                     PPCGTARFELAPYSDDVLGGTYVSSWPVPLPVPATLVVERSGGRVSGLWMSTARTRNL
                     WLERCA"
     gene            915..>2289
                     /locus_tag="VHE37_03545"
     CDS             915..>2289
                     /locus_tag="VHE37_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34628.1"
                     /translation="METMAESSSIAPPAPPLSRGLLLAFCLPALMMGFMHAPEGQVQG
                     IYAKHGGLPLAAIAGAMLFTRFFDAVTFPLIGYLSDATMRRTGSRKPWMIAGAGVTVF
                     GLWFLYHPPAQVSIEYFTGWMLVSYVGWKLTEIPYGAWAISLTSDYVQRARVQMWRAM
                     LLLLGGTLFYTVPYAAKALGLQPATDMNFATLGLNALVIAVAVPLVSLIALSRVPDGE
                     GALPQKAPARKAHVRELLRAMATSRPLLRLLGAAVPVMFLNGMSAGATYLYVDAYMGL
                     GDKYAMIQLIALPCSLLGLPFWGWLCLRYERHRVWACSMVIAAVSYAAVGFAPVGAAG
                     LPVILLLYPATVFCVGAITVALPAMMGDVLDHDRLHSGEDRAGMYSAMLAFIGKSLGG
                     VAGAAGIAVLAWFGFDATAKVQSSAGAFGIRLVMFWLPAAGFALGALLAWTFPITRAR
                     QAEIHAAL"
BASE COUNT          287 a          815 c          795 g          392 t
ORIGIN      
        1 cgtccgcgct acagcagcgg cgcgcagggc agctacgcgc tgggcctggt gcacgagcgc
       61 taccgcggcg ccgacaccat ccatcacgcc ggcgccacgc tcggctgcgt gcaccagctg
      121 ctctccgtgc cggcgcacgg cctggacctg agcatcatca gcaaccgcat ggatggcgcc
      181 gcgccggggc tggcgtggaa agtggtcgat gccgtgctgg gtgacgtgct cgcacccgcc
      241 gtgccgccgc tggcgcctgc cgagcacgaa gcggtcaccg gtcgctggta tgcgccggcc
      301 tcgcggcgcc tgtacacggt gatgcagcag ccgcagccca atcaatccgc cacgctggcg
      361 ctggccgtga atgacgggcc gatcggcctg ctccggcaga aggatggcgg ctatttcgcg
      421 gactcgccgg cgcacggcac ggtggatctg cgcgtgcccg ccggtgccgg cgcaagacct
      481 gccagcctgg aactgtccga cagcggccac gtggagcgcg tgcagcgcct gcccgacagc
      541 ggccccgatg caaccgcgct cggcgccgaa ctggtgggcc gttatcgcta tgccgatttc
      601 ggcatcgagc tcgccgtcat cgtcgaaagc gggaaacttt tcctggacct gcggccgccc
      661 tgcggcaccg cgcggtttga gctcgcgcct tattccgacg acgtgctcgg cggcacctac
      721 gtgagttcct ggccggtgcc gctgccggtt cccgccacgc tggtggtgga gcgcagcggc
      781 ggccgcgtca gcggcctgtg gatgagcacc gcgcgcacgc gcaacctgtg gctcgagcgc
      841 tgcgcctagc gccgcctgcg ccgatggacg atcaggtcat ttctccgtcg cctgctgctg
      901 ctgcgccagt tgcaatggag accatggccg aatcgtcgag cattgctccg cccgcgccgc
      961 cgctgtcgcg cggactgctg ctggcattct gcctgccggc gctgatgatg ggcttcatgc
     1021 acgcgcccga agggcaggtg cagggcatct atgccaagca tggcggcctg ccgctcgccg
     1081 ccattgccgg cgcgatgctg ttcacgcgct tcttcgacgc ggtgactttt ccgctgatcg
     1141 gctatctgtc cgacgccacc atgcgccgca ccggcagccg caagccgtgg atgatcgccg
     1201 gcgccggcgt caccgtgttc ggcctgtggt ttctctacca cccgccggcg caggtcagca
     1261 tcgaatactt caccggctgg atgctggtgt cctacgtcgg ctggaaactc accgagattc
     1321 cgtacggcgc ctgggccatc agcctgacca gcgactacgt gcagcgcgcg cgcgtgcaga
     1381 tgtggcgcgc gatgctgctg ctgctgggcg gcacgctgtt ctacaccgtg ccgtatgccg
     1441 ccaaggcgct gggcctgcag ccggccaccg acatgaattt cgccacgctg ggcctgaacg
     1501 cgctggtgat cgccgtcgcc gtgccgctgg tcagcctgat cgcgctgtcg cgcgtgccgg
     1561 acggcgaggg cgcgctgccg cagaaggcgc ccgcgcgcaa ggcacatgtg cgcgaactgc
     1621 tgcgcgccat ggccaccagc cgcccgctgc tgcggctgct gggcgccgcc gtgccggtga
     1681 tgtttctcaa cggcatgtcc gccggcgcca cctatctcta cgtcgacgcc tacatggggc
     1741 tgggcgacaa gtacgcgatg atccagctga tcgccctgcc gtgctcgctg ctggggctgc
     1801 cgttctgggg ctggctctgc ctgcgctacg aacgtcatcg cgtgtgggcc tgctcgatgg
     1861 tgatcgctgc ggtgtcgtat gccgcggttg gcttcgcgcc ggtgggcgcg gccgggctgc
     1921 cggtcatcct gctgctgtac cccgcgacgg tgttctgcgt gggcgcgatc accgtggcgc
     1981 tgcccgcgat gatgggcgac gtgctggacc atgaccgcct gcacagcggc gaggaccgcg
     2041 ccggcatgta ctcggccatg ctcgccttca tcggcaagtc gctgggcggc gtggccgggg
     2101 cggccggcat cgccgtgctg gcctggttcg gcttcgacgc caccgccaag gtgcagagca
     2161 gcgctggcgc cttcggcatc cgcctggtga tgttctggct gccggccgcg ggcttcgccc
     2221 tcggcgcgct gctggcctgg accttcccga tcacgcgcgc gcgccaggcg gaaatccacg
     2281 cggcactca
//
LOCUS       DASZZC010000154         4496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1038344,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000154 DASZZC010000000
VERSION     DASZZC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038344"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VHE37_03550"
     CDS             complement(<1..354)
                     /locus_tag="VHE37_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT34629.1"
                     /translation="MRTNKLFSIGLGCSLLAALAPAHARVPADQAAQLDTPRLTCMGA
                     ERAGSAGGVAAYTGKFFGKWPGATHDYGYEPGPYADEKPLYTVTAQNLAEHDAQLTEG
                     EKKLLQKYPQDFRMPV"
     gene            complement(404..2824)
                     /locus_tag="VHE37_03555"
     CDS             complement(404..2824)
                     /locus_tag="VHE37_03555"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT34630.1"
                     /translation="MSLRRLHLLLALPLFAPLTAGAISFNLDYGDGVSGVLNTTITAG
                     AAMRMQDRAPDLIGKSDLNPNVCFGQQSCQGVFRDQIAPARTLAAAPGAFSINGDDGD
                     LNYDRYHLTQAVAKVTQDLKLTYGDFGFFSRWLFFYDVVNNNFTQTHPNQVTAANAAQ
                     TGFGPADRNYVFLPLRALYFPVNRVYGPGTQVRDKRTDGEALAQIGTHFQLMEFVASG
                     KVNLTDDVPLTIKLGRQTVNWGESTLLVINSINQANPVNANNLFRVGFQLEEVFTPVN
                     MLFLSTELKGVNVEGYYQLEWRPVEAPTPGSFLAYQDVGTNYVGKTASVSFGGVAEDP
                     DSVARPSDNPLSLIAATSTTIQRMPDREPRNLGQYGLALKYFADTVGNGTEFGFYAMN
                     YHSKLPYASFYSANASCARREGNALGIDANSSATLLAACPDFPVVAQLTQGDARAATS
                     SAFALDSVKLQLEYPENIKMFGVSFNTTFGDFSLQGEVAYRPHAPLQVDIEDLAFAAL
                     GPTLTRCHEASLNCEGTGLVNGLPTTTGVGLDGTLYTNGSDFIPGAAVTGYKDTINVG
                     VGALGAGVGLGHLPGSARSFPNFIIPYRGGVVGENPPNSYIRGYEYFQTLQYNIGGTY
                     VAGASDNPFGASQIQSVFEIGATHVPDLPKLDVLQIDGPGTYTAASAGADGTGADGSR
                     QACSTNPSCSIGPDGLRFNPTQQKDGFTTRFSWGYRLIEIFKYESVLPGISLQPMVIF
                     WHDVQGTAPGPAENFVEGRKIIQTTLETRYKSDLSFTVGYTWYTGGGNHNLLRDRDFS
                     QAFVKYQF"
     gene            complement(3088..3885)
                     /locus_tag="VHE37_03560"
     CDS             complement(3088..3885)
                     /locus_tag="VHE37_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT34631.1"
                     /translation="MATRARKAGPRRGASALALRWRAGELQPVKFPHPVRLEALLHIE
                     RQKEALLRNTRQFVQGLPANHALLSGARGTGKSSLVKALLPEFSDDGLRLVEVHPHDL
                     ADLPDIVAPLRGRRERYVLYVDDFSVASGDPALTALKTALDGGIEEPPENVLVYATSN
                     RRHLMPEMKSDNDEYRWVEGELHPGESTEEKISLSERFGLWLSFPAYDQAQYFALVRA
                     HLSALGVREWTEEMEVLAARWALNRASRSGRVAQQFARDWAGRKNLR"
     gene            complement(3885..4433)
                     /locus_tag="VHE37_03565"
     CDS             complement(3885..4433)
                     /locus_tag="VHE37_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HVT34632.1"
                     /translation="MLKKFIAAAALCASGAALAAGPAFTLGSTDVAANQKMADKQVFN
                     GFGCTGGNVSPALAWKNAPAATKSFALTVYDPDAPTGSGWWHWVVYNIPADTTALPAD
                     AGSGKGLPAGAVQGRTDFGSAGWGGPCPPAGDKPHHYIFTLHALKTDKLDVPADASAA
                     MVGFMIHANEIGKAKFTALYNR"
BASE COUNT          688 a         1430 c         1588 g          790 t
ORIGIN      
        1 caccggcatg cggaaatcct gcggatattt ctgcagcagc ttcttctcgc cctcggtcag
       61 ctgcgcgtcg tgctcggcca ggttctgcgc ggtgacggtg tacagcggct tctcgtcggc
      121 gtacgggccc ggctcgtagc cgtagtcatg cgtggcgccg ggccacttgc cgaagaattt
      181 tccggtgtac gcggccacgc cgcccgcgct gccggcgcgc tccgcgccca tgcaggtgag
      241 cctgggcgtg tcgagctggg cggcctggtc ggcgggaact cttgcatgag ccggcgcaag
      301 ggctgccagc agcgagcatc ccaggccgat cgagaagagt ttgttcgtgc gcatgggcag
      361 cgattacacg cccgccgatg gcacgtgcca tcacgcgtat ggcttagaac tggtacttca
      421 caaacgcctg cgaaaaatcc ctgtcgcgca gcaggttgtg gttgccgcca ccggtgtacc
      481 aggtatagcc gacggtgaac gacaggtcgg acttgtagcg cgtctccagc gtggtctgga
      541 tgatcttgcg gccttcgacg aagttttccg ccgggccggg cgcggtgccc tgcacgtcgt
      601 gccagaagat caccatgggc tgcaggctga tgccgggcag cacgctttcg tacttgaaga
      661 tctcgatcag gcggtagccc caggagaagc gcgtggtgaa gccatccttc tgctgcgtgg
      721 ggttgaagcg caggccgtcg gggccgatgg agcaggacgg attggtcgag caggcctggc
      781 gcgagccgtc ggcgccggtg ccgtcggcgc ccgcactggc cgcggtgtag gtgccgggtc
      841 cgtcgatctg cagcacgtcg agcttgggca ggtcgggcac gtgggtggcg ccgatttcga
      901 acacgctctg gatctggctg gcgccgaacg ggttgtcgga cgcgccggcc acgtaggttc
      961 cgccgatgtt gtactgcagc gtctggaaat attcgtagcc gcggatgtag ctgttcggcg
     1021 ggttctcgcc gaccacgccg ccgcggtacg ggatgatgaa gttggggaac gagcgcgccg
     1081 agccgggcag gtggcccagg ccgacgccgg cgccgagcgc gccgacgccc acgttgatgg
     1141 tgtccttgta gccggtgacg gcggcgccgg gaatgaaatc gctgccgttg gtgtacagcg
     1201 tgccgtcgag gccgacgccg gtggtggtcg gcaggccgtt gaccaggccg gtgccttcgc
     1261 agttgagcga ggcttcgtgg cagcgcgtga gcgtggggcc cagcgcggcg aaggccaggt
     1321 cctcgatgtc cacctgcagc ggcgcgtgcg ggcggtaggc cacttcaccc tgcagcgaga
     1381 aatcgccgaa ggtggtgttg aacgacacgc cgaacatctt gatgttctcg gggtactcca
     1441 gctgcagctt caccgagtcg agcgcgaacg cgctgctggt ggcggcgcgc gcatctcctt
     1501 gcgtgagctg cgccaccacc gggaaatccg ggcacgcggc gagcaacgtg gccgagctgt
     1561 tggcgtcgat gcccagcgcg ttgccctcgc gccgcgcgca cgaggcgttg gccgagtaga
     1621 agctggcgta cggcagcttg gagtgatagt tcatggcgta gaagccgaac tcggtgccgt
     1681 tgcccaccgt gtcggcgaag tacttgagcg cgagcccgta ctggccgagg ttgcgcggct
     1741 cgcggtcggg catgcgctgg attgtcgtgc tggtggcggc gatcagcgac agcgggttgt
     1801 ccgacggccg cgccaccgag tcgggatctt ccgccacgcc gccgaacgac accgacgcgg
     1861 tcttgccgac gtagttggtg cccacgtcct ggtaggcgag gaacgaaccc ggcgtcggcg
     1921 cttccaccgg ccgccactcc agctggtaat agccctcgac gttgacgccc ttgagctcgg
     1981 tggacaggaa cagcatgttc accggcgtga acacttcctc cagctggaag cccacgcgga
     2041 acaggttgtt ggcgttgacc gggttggcct ggttgatgct gttgatcacc agcagggtcg
     2101 attcgcccca gttcaccgtc tgccggccga gcttgatggt cagcggcacg tcgtcggtca
     2161 ggttcacctt gccgctggcg acgaattcca tcagctggaa atgcgtgccg atctgcgcca
     2221 gcgcctcgcc gtcggtgcgc ttgtcgcgca cctgcgtgcc cggcccgtag acgcggttca
     2281 ccgggaagta cagcgcgcgc agcggcagga acacgtagtt gcggtcggcc gggccgaagc
     2341 cggtctgcgc cgcgttcgcc gccgtcacct ggttcgggtg ggtctgggtg aagttgttat
     2401 tgaccacgtc gtagaagaac agccagcggc tgaagaaacc gaaatcgccg taagtcagct
     2461 tcaggtcctg cgtgaccttg gccacggcct gcgtcaggtg ataacggtcg taattgaggt
     2521 cgccgtcgtc gccgttgatg gagaacgcgc cgggcgccgc ggccagcgtg cgcgccggcg
     2581 cgatctggtc gcggaacacg ccctggcacg actgctggcc gaagcacacg ttagggttga
     2641 ggtcggactt gccgatcagg tccggcgcgc gatcctgcat gcgcatggcc gcgccggcgg
     2701 tgatcgtggt gttgagcacg ccggaaacgc cgtcgccgta atcgaggttg aacgagatgg
     2761 cgccggcggt gagtggcgcg aacagcggca aggcgagcag caggtgcaga cggcgcagcg
     2821 acatacgcag gactcccgac ggtgacgaag ctttacggtt ctcctccgcc gcccactctg
     2881 tgcccccggt gggatgtgcg cttccgcgag cggaatgcgc ctggcttttc gcgggcgcca
     2941 caatatccgc gcgcatcgcg tttgaacagg cttgcgcggg cttttttggc ctcaaccgca
     3001 cgggcatttg acatgttact aattatttat caaaagtaat ttcgatcaac aattattaac
     3061 accgcaaaag ggcatgcggt gacggcctca gcgcaggttt ttgcgccccg cccagtcgcg
     3121 cgcgaactgc tgcgccacgc gcccggagcg cgacgcgcgg ttgagcgccc agcgcgcggc
     3181 cagcacttcc atttcctccg tccactcgcg cacgcccagc gcggacaggt gcgcgcgcac
     3241 cagcgcgaag tactgcgcct ggtcgtacgc gggaaacgac agccacaggc cgaagcgctc
     3301 cgacagcgag atcttctcct cggtcgattc gcctgggtgc agttcgccct cgacccagcg
     3361 gtactcgtcg ttgtcgctct tcatctccgg catcaggtgc cgacggttcg acgtggcata
     3421 gaccagcacg ttctccggcg gctcctcgat gccaccgtcc agcgcggtct tcagcgcggt
     3481 gagcgcgggg tcgccgctgg cgacggagaa atcgtcgacg tagagcacgt agcgctcgcg
     3541 ccgcccgcgc agcggcgcca cgatgtccgg caggtcggcc aggtcgtgcg gatgcacttc
     3601 caccaggcgc aggccgtcat cggaaaattc cggcagcagc gctttcacca gcgacgactt
     3661 gccggtgccg cgcgcgccgg acagcagcgc gtggttggcc ggcaggccct gcacgaactg
     3721 ccgtgtgttg cgcagcagcg cttccttctg ccgctcgatg tgcagcagcg cttcgagccg
     3781 caccgggtgc ggaaacttca ccggctgcag ttcacccgcg cgccagcgca aggcgagcgc
     3841 ggacgcgccg cggcgcgggc ccgccttgcg tgcgcgcgtt gccatcagcg gttgtagagc
     3901 gcggtgaact tcgccttgcc gatctcgttg gcgtggatca tgaagccgac catggcggcg
     3961 ctggcgtcgg cgggcacgtc gagcttgtcg gtcttgagcg catgcagggt gaagatgtag
     4021 tggtgcggct tgtcgccggc aggcgggcac ggaccgcccc agccggcgct gccgaagtcg
     4081 gtgcggccct gcaccgcgcc cgcgggcagg cccttgccgg agccggcatc ggccggcagc
     4141 gcggtggtgt cggcaggaat gttgtagacc acccagtgcc accagccgct gccggtgggc
     4201 gcgtccgggt cgtacaccgt cagcgcgaaa ctcttggttg ccgcgggcgc gttcttccag
     4261 gccagcgccg gcgagacatt gccgccggtg cagccgaagc cgttgaacac ctgcttgtcc
     4321 gccatcttct ggttggccgc cacgtcggtg ctgcccagcg tgaaggcggg gccggcggca
     4381 agcgcggcgc cgctggcaca cagcgcggcg gcggcgatga actttttcag catgggaatc
     4441 ctcctgattc atgtggggca gggcgcggca ttctaagcgc tgcctggggc agaatg
//
LOCUS       DASZZC010000155         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_846868,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000155 DASZZC010000000
VERSION     DASZZC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846868"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VHE37_03570"
     CDS             <1..1211
                     /locus_tag="VHE37_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HVT34633.1"
                     /translation="GSMVAGFIATRWGRRSALLLAALLFVLSSLGSALPELGFGGIGA
                     MGPAALPMFIAYRILGGTGIGLASLVSPMYIAEIAPRAVRGRLVSYNQMAIVTGIVGV
                     YFVNWAIAAQGDDAWLHAVGWRWMFASAMVPAAAFFALLLSAPDSPRWLVLRGRDAEA
                     DALLHRLEGDAEAVQAMAEIRASLVVPTQPLLSYGIGVIVIGILLSVFQQAVGINAVL
                     YYAPSIFQNMGLGGNAALLQTVLVGSVNFAATLIAIFTVDRWGRKPLLIGGGLIMGAA
                     MAWLALCFQLSALGSGALLAMLLYIAGFALSWGPVCWVLLAEIYPNSIKGKALSIAVA
                     AQWLANVIVSWSFKVIDGDSALIAAFHHGFAYWMYAGFSLLSALFVWRLVPETKGRTL
                     ESIEALWKRP"
     gene            complement(1323..1748)
                     /locus_tag="VHE37_03575"
     CDS             complement(1323..1748)
                     /locus_tag="VHE37_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVT34634.1"
                     /translation="MEAAKAMLAPTIALVLWSAVIWFWMYATRIPAIAQMKMRLDPNA
                     PRGEQMSQLPAHVRWKADNYNHLMEQPTLFYAVALTLAILGDHSAASVALAWGYVLLR
                     VVHSLWQSLRNVIIVRFALFALSSLLLWALAARAALQVF"
BASE COUNT          267 a          583 c          636 g          353 t
ORIGIN      
        1 tcggctcgat ggtcgcgggc ttcatcgcga cgcgctgggg ccggcgcagc gcgctgctgc
       61 tggcggcgct gctgttcgtg ctgtcgtcgc tcggctcggc gctgccggaa ctgggcttcg
      121 gcggcatcgg tgcgatgggc ccggcggcgc tgccgatgtt catcgcctac cgcattctcg
      181 gcggcaccgg catcggcctg gcctcgctgg tgtcgccgat gtacatcgcc gagatcgcgc
      241 cgcgcgcggt gcgcgggcgg ctggtgtcgt acaaccagat ggccatcgtc accggcattg
      301 tcggcgtgta cttcgtcaac tgggcgatcg ccgcgcaggg cgatgacgcc tggctgcacg
      361 cggtgggctg gcgctggatg ttcgcctcgg ccatggtgcc ggcggccgcg ttcttcgcgc
      421 tgctgctgtc cgcgcccgac agcccgcgct ggctggtgct gcgcggccgc gacgccgagg
      481 ccgacgcgct gctgcatcgc ctggaaggcg acgccgaggc ggtgcaggcc atggcggaaa
      541 tccgcgcgag cctggtggtg ccgacgcagc cactgctgag ctacggcatc ggcgtgatcg
      601 tcatcggaat tttattgtcg gtgttccagc aggcggtggg catcaacgcg gtgctgtact
      661 acgccccgtc gatcttccag aacatgggcc tgggcggcaa cgccgcgctg ctgcagacgg
      721 tgctggtggg ctcggtgaat ttcgccgcca cgctgatcgc catcttcacc gtcgaccgct
      781 ggggcaggaa gccgctgctg atcggcggcg ggctgatcat gggcgcggcg atggcctggc
      841 tggcgctgtg cttccagctg tcggcgctgg gcagcggcgc gctgctggcc atgctgctgt
      901 acatcgccgg cttcgcgctg tcgtggggcc cggtgtgctg ggtgctgctg gcggagatct
      961 acccgaactc gatcaagggc aaggcgctgt ccatcgcggt ggctgcgcaa tggctggcca
     1021 acgtgatcgt gtcgtggtcg ttcaaggtga tcgacggcga cagcgcgctg atcgccgcct
     1081 ttcaccacgg cttcgcctac tggatgtacg ccggcttctc gctgctgtcg gcgctgttcg
     1141 tctggcgcct ggtgccggag accaagggcc gcacgctgga atcgatcgaa gcgctgtgga
     1201 agcggccgtg aaaaccctcc accgcaccgg catttggtat gttataaatt actatattaa
     1261 tttattattg ataaatattt tataacacgc tggatgccgg tgcggtgcaa tgattttttg
     1321 cttcagaaca cctgcaacgc cgcgcgcgcc gccagcgccc acagcagcag cgacgacagc
     1381 gcgaacagcg cgaagcgcac gatgatcacg ttgcgcagcg actgccacag gctgtgcacc
     1441 acgcgcagca gcacgtagcc ccaggccagc gccacgctag cggcggagtg gtcgccgagg
     1501 atggccagtg tcagcgctac cgcgtagaac agcgtgggct gctccatcag gtggttgtag
     1561 ttgtcggcct tccagcgcac gtgggccggc agctgcgaca tctgctcgcc gcgcggcgca
     1621 ttcggatcga gccgcatctt catctgcgcg atggccggaa tgcgcgtggc gtacatccag
     1681 aaccagatca ccgcggacca cagcaccagc gcaatggtcg gcgccagcat tgcctttgca
     1741 gcttccatcg tgatttctcc cttgttgtgt gccggcattg tgtccgacgc ggcaggaatg
     1801 cggtaggaat gctctagggc gcgcacgcac gcatcaggc
//
LOCUS       DASZZC010000156         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_203423,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000156 DASZZC010000000
VERSION     DASZZC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203423"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VHE37_03580"
     CDS             complement(<1..557)
                     /locus_tag="VHE37_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT34635.1"
                     /translation="MLDLPIVDPHQHLWDFTRAHYGWLMDTPLAHNPAGDCTPIAKPY
                     AIEDYLADTRGYQISKVVHVEAGADPANAIRETQWLSEVAQRHGYPQGIVAYCALQRP
                     DAANVLAAHREHANVRGIRQIINFHRDPLKTYTQRDLLDDAAWRKGYALLAKHDLSFD
                     LQIYPGQMPAAAELAARHENTRLVL"
     gene            complement(731..1492)
                     /locus_tag="VHE37_03585"
     CDS             complement(731..1492)
                     /locus_tag="VHE37_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT34636.1"
                     /translation="MTGQRAVYAGRFAGQAAVIAGGASGVGKELAARLTAEGAKVSLW
                     DFDAEALARSASETGAAHTRVVDVADLGQVEAAAADSWHALGRIDVLVCSAGITGPTA
                     SLCDYPAADWRRVIDINLNGTFYCCKSVVPYMLRNDYGRVVNISSVAGKEGNPNASAY
                     STAKAGVIGLTKSLGKELAKTGVRVNCVTPATFRSPILKQLTQSQIDYMLTRIPIGRI
                     GEAEEVAALVSWLCSRECSFSTGATFDISGGRTTY"
     gene            complement(1489..>1821)
                     /locus_tag="VHE37_03590"
     CDS             complement(1489..>1821)
                     /locus_tag="VHE37_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVT34637.1"
                     /translation="CDTFGPVGPWLVTADEVGDYNNLDMWLEINGQRMQTGNTRTMIF
                     KVPELVAYLSRFMTLQPGDIITTGTPPGVGLGMKPNPVYLKAGDTMHVYIEKLGEQRQ
                     RVVPWSTS"
BASE COUNT          287 a          581 c          622 g          331 t
ORIGIN      
        1 ttcagcacca gccgcgtgtt ttcatggcgc gccgcgagtt cggcggcggc cggcatctgc
       61 ccgggataaa tctgcaggtc gaacgaaaga tcgtgctttg ccagcagcgc gtagcctttc
      121 ctccacgcgg cgtcgtccag caggtcgcgc tgcgtatagg tcttcagcgg atcgcggtgg
      181 aagttgatga tctggcggat gccgcgcacg ttggcgtgct cgcggtgcgc ggccagcacg
      241 ttggcggcat cgggccgctg cagcgcgcag taggcgacga tgccctgcgg gtagccgtgg
      301 cgctgcgcca cctcggacag ccattgcgtt tcgcggatgg cattggcggg atcggcgccg
      361 gcctcgacat gcaccacctt gctgatctgg tagccgcgcg tgtcggcgag gtaatcctcg
      421 atggcatagg gtttggcgat cggcgtgcaa tcgcccgccg ggttgtgcgc cagcggcgta
      481 tccatcagcc agccgtaatg cgcgcgggtg aaatcccaga gatgctgatg cggatcgacg
      541 atgggcaagt ccagcatctc gcgttcctcc tgatgctgcg acatcagagc tgaaaagcaa
      601 aaaaaccgtc attcccgcga aggccggaat ccagtttttt cccgctcgat gggctgccgt
      661 caaaactgga tccccgcctg cgcggggatg acggctgcct tgtttgcggc gcgggcggca
      721 tatctatcaa tcagtacgtg gtgcgtccgc cggaaatgtc gaaggtggcg ccggtggaga
      781 acgagcactc gcggctgcac agccacgaca ccagtgcggc gacttcctcg gcctcgccga
      841 tgcggccgat gggaatgcgc gtgagcatgt agtcgatctg cgactgcgtc agctgcttca
      901 agatcgggct gcggaacgtc gccggcgtca cgcagttcac ccgcacgccg gtcttggcca
      961 gctccttgcc gagcgacttg gtcaggccaa tcacgccggc cttcgcggtg gaataggcgg
     1021 aagcattggg attgccttcc ttgccggcga ccgacgagat gttgaccacg cggccgtagt
     1081 cgttgcgcag catgtacggc accaccgact tgcagcagta gaaggtgccg ttgaggttga
     1141 tgtcgatgac gcggcgccag tcggctgcgg ggtaatcgca cagcgatgcg gtcgggccgg
     1201 tgatgccggc cgagcacacc agcacgtcga tgcggcccag cgcatgccag ctgtcggcgg
     1261 cggcggcttc cacctggccg aggtcggcga cgtcgaccac gcgcgtatgc gcggcgccgg
     1321 tttcgctcgc gctgcgagcc agcgcttccg catcgaaatc ccacaggctg accttcgcgc
     1381 cttccgccgt cagccgcgcg gccagttcct tgcccacgcc ggaagcgccg ccggcgatga
     1441 ccgcggcctg gccggcgaag cggccggcgt ataccgcgcg ctggcctgtc atgacgtgct
     1501 ccacggcacc acgcgctggc gctgctcgcc cagcttctcg atgtacacgt gcatggtgtc
     1561 gccggccttc aggtaaaccg ggttgggttt catgcccagc ccgacgcccg gcggcgtgcc
     1621 ggtggtgatg atgtcgccgg gctgcagcgt catgaagcgc gagagatagg cgaccagctc
     1681 cggcaccttg aagatcatgg tgcgcgtgtt gccggtctgc atgcgctggc cgttgatctc
     1741 cagccacatg tcgaggttgt tgtagtcgcc cacctcatcg gcagtgacca gccaggggcc
     1801 gaccgggccg aaggtgtcgc a
//
LOCUS       DASZZC010000157         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_203510,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000157 DASZZC010000000
VERSION     DASZZC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203510"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2138)
                     /gene="dnaE"
                     /locus_tag="VHE37_03595"
     CDS             complement(<1..>2138)
                     /gene="dnaE"
                     /locus_tag="VHE37_03595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVT34638.1"
                     /translation="DEEWVQAAAALARHRGVPLVASNDVRFLARDEFDAHEARVCIAQ
                     GRVLNDDRRPRDYTAEQYLKSADEMAALFHDLPEAVANTVEIARRCSLKLTLGKTHLP
                     QFPAPAGHDADSWVRHCAEEGLARRLEQIPPSQPREVYRQRLEYELGIIRQMGFAGYF
                     LVVADFIAWAKSHGCPVGPGRGSGAGSLVAYALGITDLDPLPYQLLFERFLNPERVSL
                     PDFDVDFCMDNRDRVIAYVQEKYGRDRVGQIITYATMAARGVVRDVARVMGHGYGFGD
                     SIAKLIPGIPGATLADALDSVPELKQRYRDEEDTRAVLDLALELEGITRGVGVHAGGV
                     VIAPNPLTDYAPLYCEPGGGGLRTQFDMKDLESVGLVKFDFLGLKTLTILQTAVDMLE
                     HKTGEKIDLLKIPLNDAATYQLYSSGDTSAVFQMESPGMQKASRDLKPDTFEDIIALV
                     SLYRPGPMELIPDYCARKRGDAKVEYLHPDMEKVLAPTYGIFVYQEQVMQMGQLLAGY
                     SLGGADLLRRAMGKKIAAEMAKQREVFQQGATARGVEAQVATQVFDLMEKFANYGFNK
                     SHAAAYALVSYQTAWLKTHYPAEFMASVLSCEMNHTDTVVSMLDECRRMGLKVHAPDI
                     NTSEFRFTVPAAGEIRYGLGAIKGMGEAALEGVLAERAKNGPFADLFDFCRRVDLHKV
                     NKRVLEAMIFSGAFDGFGLNRASLHKTLPK"
BASE COUNT          335 a          736 c          703 g          364 t
ORIGIN      
        1 ccttgggcag cgtcttgtgc agcgatgcgc ggttcaggcc gaagccatcg aacgcgccgg
       61 aaaaaatcat ggcctccagc acgcgcttgt tcaccttgtg caggtccacg cgccggcaga
      121 aatcgaacag gtcggcgaag gggccattct tcgcgcgctc ggccagcacg ccttccagcg
      181 cggcctcgcc catgcccttg atcgcgccca aaccgtagcg gatttcgccc gccgccggca
      241 cggtgaagcg gaactcgctg gtattgatgt ccggcgcgtg caccttaagc cccatgcgcc
      301 ggcactcgtc gagcatgctg accacggtgt cggtgtggtt catttcgcac gacagcaccg
      361 aggccatgaa ctccgccggg tagtgcgtct tcagccacgc ggtctggtac gagaccagcg
      421 cgtatgcggc cgcgtgcgac ttgttgaagc cgtagttggc gaacttctcc atcaggtcga
      481 acacctgcgt ggccacctgc gcctccacgc cgcgcgcggt ggcgccctgc tggaacacct
      541 cgcgctgctt ggccatttcc gccgcgatct tcttgcccat cgcgcggcgc agcaggtcgg
      601 cgccgccgag cgagtagccg gccagcagct ggcccatctg catcacctgc tcctggtaga
      661 cgaagatgcc gtaggtgggc gccagcacct tctccatgtc cgggtgcagg tattcgacct
      721 tcgcatcgcc gcgcttgcgc gcgcagtagt cggggatcag ctccatcggg cccggccggt
      781 acaacgaaac cagcgcgatg atgtcctcga aggtgtcggg cttgagatcg cgcgaggcct
      841 tctgcatgcc cggcgattcc atctggaaca ccgcgctggt atcgccgctg gaatacagct
      901 ggtaggtcgc cgcatcgttg agcgggattt tcagcaggtc gatcttctcg ccggtcttgt
      961 gctcgagcat gtcgacggcg gtctgcagga tggtgagcgt tttcaatccc aggaaatcga
     1021 acttcaccag gccgaccgat tccaggtcct tcatgtcgaa ctgcgtgcgc aggccgccgc
     1081 cacccggttc gcagtacagc ggcgcgtagt cggtgagcgg gttgggcgcg atgaccacgc
     1141 cgcccgcgtg cacgccgacg ccgcgggtga tgccttcgag ttccagcgcc aggtccagca
     1201 ccgcgcgcgt gtcctcttca tcccggtagc gctgcttgag ctcgggcacc gagtcgagcg
     1261 catcggccag cgtggcgccc ggaatgccgg ggatgagctt ggcgatcgaa tcgccgaagc
     1321 catagccgtg gcccatcacg cgcgccacgt cgcgcaccac gccacgcgcg gccatggtgg
     1381 cgtaggtgat gatctgccct acgcggtcgc ggccgtactt ttcctgcacg taggcgatca
     1441 cgcggtcgcg gttgtccatg cagaagtcga cgtcgaagtc gggcagcgac acgcgctcgg
     1501 gattgaggaa gcgctcgaac agcaactggt acggcagcgg atcgaggtcg gtgatgccca
     1561 gcgcgtatgc caccagcgag ccggcgccgg agccgcggcc cgggcccacc gggcagccgt
     1621 gcgatttggc ccaggcgatg aagtccgcca ccaccaggaa gtaaccggca aagcccatct
     1681 gcctgatgat gcccagctcg tactcgagcc gctgccggta cacctcgcgc ggctgcgacg
     1741 gcgggatctg ctcaagccgc cgcgccagtc cttcctcagc gcagtgccgc acccaggaat
     1801 cggcgtcgtg gccggccggc gccgggaact gcggcaggtg cgtcttgccc agcgtgagct
     1861 tcagcgagca gcgccgcgca atctccaccg tgttggcgac ggcctcgggc agatcgtgga
     1921 acagcgccgc catttcgtcg gcggatttca ggtactgctc ggcggtgtaa tcgcgcgggc
     1981 ggcggtcgtc gttgagcacg cgcccctgcg cgatgcacac gcgcgcctcg tgcgcgtcga
     2041 actcgtcgcg cgccaggaag cgcacgtcgt tgctcgccac cagcggcacg ccgcgatggc
     2101 gcgccagcgc cgcggccgcc tgcacccact cctcgtcg
//
LOCUS       DASZZC010000158         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_463563,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000158 DASZZC010000000
VERSION     DASZZC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463563"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VHE37_03600"
     CDS             complement(<1..456)
                     /locus_tag="VHE37_03600"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVT34639.1"
                     /translation="MSADIPLPAALPRRLIAALYDALLLLALWMITALADAIVRRISA
                     LPPNINALRLCLFLVGLAFFGWFWTHGGQTLGMRAWRLQVRRDDGAPLRWPVAAVRYA
                     AAYLSWALAGIGILWSLVDARRRCLHDIVSGTEVVLLPKAASTHAGEPGQ"
     gene            756..>3329
                     /locus_tag="VHE37_03605"
     CDS             756..>3329
                     /locus_tag="VHE37_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HVT34640.1"
                     /translation="MSLRMSAYASLASLFALVTLAGCGHGGANTTTTTATGGGGDLST
                     SGRHFVVKPGASATADMVSAMIQAGPGDVIEFGCGYFELTSSLLLSHTEDVLVKGCGK
                     DKTVLSFKNNNAPEGILVDSVNGLTLQDLTVIDTGGNGIELRGVDHATLQRVRAMWSS
                     GGGRASSDPITADNFADKIHVPCTHPATQSPDVPESNPANTTSPDYTVSKLSGRYGIY
                     PVSSSNVLIEDSESIGASDAGIYIGQTNNALFRHNRAAFNVFGMEIENVQGGEYDGNL
                     AECNTGGFLIYDIDGLRQYGDRSVMMNNISRMNNTYNFTSGGFVGNVPPGSGMITLAY
                     DRVDIHDNEFTDNNTAGIIHTSYEIFPEGAGRPSEHRIDWYTEGLHIWHNKFNNNGNG
                     LPLPTTTDLQTQNLAHLLPALVGAKNAAGCLLPTQAAQCVAGAALPFRGAHIIWDGLL
                     DTYNAGCAYPLDASGNPVPHDARGKPIMTNETPDPSCHYNAYKFDTTKPAAPRVMPDW
                     FSCIDDDNTFSSDSLAYANFHGTRGLEVVIAALSASPGPNTVLDAIKALDPVALTQFP
                     SSFDMSPHKCVAQYGKNLPALPPVVIPPFVRSGDYDPAPSDALIAQLCGASVPAGQVN
                     FDAAKVNCPTLDQYHLFSDAEDPTSLPNTGGTPFSLNTKLFSDYAVKYRVLFLPPGTH
                     AVYGDAASNGVNATVVFPAGTIIAKTFSFADEAHHAETPLETRLLIKRVNSKGAAHWD
                     GIPYTWSTENGHRVARLALGGATASVSWDTTDIDSNQHHTGSTTSYLVPNGNQCLSCH
                     SNDDKEPGSSPIGPKIRFLNRAYRPESTFTSSRAQQPDVLGQNQIAYWCGHGIMIGCP
                     AD"
BASE COUNT          645 a         1177 c         1027 g          480 t
ORIGIN      
        1 ctggcccggc tcgcccgcat gagttgacgc ggccttgggc agcagcacga cctcggtgcc
       61 gctgacgatg tcgtgcaggc agcggcggcg cgcgtcgacc agcgaccaca ggatgccgat
      121 accggccagc gcccacgaca ggtacgcggc ggcgtagcgc accgcggcca ccggccagcg
      181 cagcggcgcg ccgtcgtcgc ggcgcacctg cagccgccac gcgcgcatgc ccagcgtctg
      241 cccgccatgg gtccagaacc agccgaagaa agccaggccg accaggaaca agcacagccg
      301 cagcgcgttg atgttcggcg gcagcgcgga gatgcggcgc acgatggcat cggccagcgc
      361 ggtgatcatc cacagcgcca gcagcagcag cgcgtcgtag agcgcggcga tcaggcggcg
      421 cggcagggcg gcggggagcg ggatgtcggc ggacacggaa gatggcgcgg aaaaaattcg
      481 ggcgcggatt gtcgcgcatt cgtgcagtcg cggcgaaaac ttgccggcag gctttcgggc
      541 atcaccacga gctcccaccg caccgcgcaa acgattgtta ttaaaaatac aataaaagtt
      601 aaaacatatt atatttaata acacacagga tggccgtgcg gttaagtgat tttctcctgc
      661 acttgcagca gtcgcggcgc atccgcgccg cttagaatcg cagccagaaa aaatctgcgg
      721 ccagtgcgcc gcaatccgac ttcggggagt tacacatgtc cttgcgcatg tctgcatatg
      781 cgtcgctggc gtccttgttt gccctcgtca ccctggccgg ttgcggtcac ggcggcgcca
      841 ataccaccac cacgaccgcc accggcggcg gcggcgatct ctccaccagc ggccggcatt
      901 tcgtggtcaa gcccggcgcc agcgccaccg ccgacatggt cagcgccatg atccaggcgg
      961 ggccgggcga tgtgatcgag ttcggctgcg gctatttcga gctgacctct tcgctgctgc
     1021 tgtcgcacac cgaggacgtg ctggtgaagg gctgcggcaa ggacaagacg gtgctgtcgt
     1081 tcaagaacaa caacgcgccg gaaggcatcc tggtggacag cgtcaacggg ctgacgctgc
     1141 aggatctcac cgtgatcgac accggcggca acggcatcga gctgcgcggc gtcgaccacg
     1201 ccacgctgca gcgcgtgcgc gccatgtggt cgtccggcgg cggccgcgcc agcagcgatc
     1261 cgatcaccgc cgacaacttc gccgacaaga tccacgtgcc gtgcacgcac ccggccacgc
     1321 agagcccgga tgtgccggag agcaacccgg ccaacaccac ctcgccggac tacacggtga
     1381 gcaagctgtc cggccgctac ggcatctacc cggtgtcgtc gtccaacgtg ctgatcgagg
     1441 attccgagtc gattggcgcg tccgacgccg gcatctacat cggccagacc aacaacgcgc
     1501 tgttccgcca caaccgcgcg gcgttcaacg tcttcggcat ggagatcgag aacgtgcagg
     1561 gcggcgagta cgacggcaac ctggccgagt gcaacaccgg cggcttcctg atctacgaca
     1621 tcgacggcct gcgccagtac ggcgaccgct cggtgatgat gaacaacatc tcgcgcatga
     1681 acaacaccta caacttcacc tcgggcggct tcgtcggcaa cgtgccgccg ggcagcggca
     1741 tgatcacgct ggcctatgac cgcgtcgaca tccacgacaa cgagttcacc gacaacaaca
     1801 ccgccggcat cattcacacc agctacgaga tcttccccga aggcgccggc cggcccagcg
     1861 agcaccgcat cgactggtac accgagggcc tgcatatctg gcacaacaag ttcaacaaca
     1921 acggcaacgg cctgccgctg cccaccacca ccgacctgca gacgcaaaac ctggcgcacc
     1981 tgctgccggc gctggtcggc gccaagaacg ccgccggctg cctgctgccg acgcaggccg
     2041 cgcaatgcgt ggccggcgca gcactgccat tccgcggggc acacatcatc tgggacggcc
     2101 tgctcgatac ctacaacgcc ggatgcgcct atccgctgga tgccagcggc aacccggtgc
     2161 cgcacgacgc gcgcggcaag ccgatcatga ccaacgagac gccggacccg tcgtgccact
     2221 acaacgccta caagttcgac accaccaagc ccgccgcgcc gcgcgtgatg ccggactggt
     2281 tctcgtgcat cgatgacgac aacaccttct ccagcgacag cctggcctat gccaacttcc
     2341 atggcacgcg cggattggaa gtggtgattg cggcgctgtc cgcgtcgccc gggcccaaca
     2401 ccgtgctcga tgcgatcaag gcgctggacc cggtggcgct gacccagttc ccctcgtcgt
     2461 tcgacatgag tccgcacaag tgcgtggcgc agtacggcaa gaacctgccg gcgctgccgc
     2521 cggtggtcat cccgccgttc gtgcgcagcg gtgactacga cccggcgccc agcgatgcgc
     2581 tgatcgcgca gctgtgcggc gccagcgtgc cggcagggca ggtgaacttc gacgcggcca
     2641 aggtcaattg ccccacgctc gaccagtacc acctgttcag cgacgcggaa gacccgacca
     2701 gcctgcccaa caccggcggc acgccgtttt cgctcaatac aaagttgttc tccgattacg
     2761 cggtgaaata ccgcgtgctg ttcctgccgc ccggcacgca tgcggtgtac ggcgacgcgg
     2821 ccagcaacgg cgtcaacgcc accgtggtgt ttccggccgg caccatcatc gccaagacct
     2881 tctccttcgc cgacgaggcg caccacgcgg agacgccgct ggaaacgcgc ctgctgatca
     2941 agcgcgtgaa ctccaagggg gccgcgcact gggacggcat tccgtatacc tggagcacgg
     3001 aaaacggtca tcgcgtggcg cggctggcgc tgggcggcgc gacggcgtcg gtcagctggg
     3061 acaccaccga catcgactcc aaccagcacc acaccggcag caccaccagc tacctggtgc
     3121 ccaacggcaa ccagtgcctg tcgtgccatt ccaacgacga caaggaaccg ggctcgtcgc
     3181 ccatcgggcc caagatccgc ttcctcaacc gcgcctaccg gccggagtcc accttcacct
     3241 cgtcgcgcgc gcagcagccc gacgtgctcg ggcagaacca gatcgcgtac tggtgcggcc
     3301 acggcatcat gatcggctgc ccggcagac
//
LOCUS       DASZZC010000159         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_14583,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000159 DASZZC010000000
VERSION     DASZZC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14583"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VHE37_03610"
     CDS             <1..569
                     /locus_tag="VHE37_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVT34641.1"
                     /translation="HALDSAQQQVNAAQQQAASVLAMLGGDADVAADEHPSVKQAQAE
                     LDKAQLDLSYTRITAPDDGIVTKVEQLQAGDAIAAGTPVFALISTHDVWVEANFKESQ
                     LTHMQAGQPAEIRIDTYPGRRFRAHVASIAPGTGSQFSALPAENATGNWVKVVQRLPV
                     RLELENAPAELPLHTGLSADVEVDTQPK"
     gene            573..>1953
                     /locus_tag="VHE37_03615"
     CDS             573..>1953
                     /locus_tag="VHE37_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HVT34642.1"
                     /translation="MHTHDASGRPLNRMMITCTVMLTSILQTLDNTIANVALPHMQGT
                     LSATQDQMTWVLTSYIVAAAVMTPLTGWLAGAIGRKRLFLASVAGFTVTSMLCGLAQS
                     LPQIVLFRFLQGMAGAALVPMSQAVLFDINPPERHGKAMAAWGQGVLLGPMIGPILGG
                     WLTDNYSWRWVFYINLPLGILAFLGVLAYLPEGEKRRTRFDFFGFALLSIFIAALQLV
                     LDRGPLQDWFGSTEIWIEAAAAGLALYLFAVHSATSREPFFRPALFKDRNFLTGNVFI
                     FVVGIVLFATLALIPPMLQELMGYPVYQAGLLTAPRSIGSLGAMLIAGRVVGKVDSRL
                     IIAGGFALTCFSLWQMTRFDLAMNGWPIFWSGVFQGLGTGIAYVPMAALTFATLDPGL
                     RNEGTALFSLTRNIGSSIGISTVAALLTRNTQIVHSTLAEHVSPYGLALRHPQLAAQL
                     AHGGAAAVNA"
BASE COUNT          287 a          691 c          647 g          328 t
ORIGIN      
        1 gccatgcgct ggacagcgcg cagcagcagg tgaacgccgc gcagcagcag gcggccagcg
       61 tgctggccat gctcggcggc gatgccgatg tcgctgccga tgagcacccg tcggtgaagc
      121 aggcgcaggc cgagctggac aaggcgcagc tcgacctgtc ctacacgcgt atcaccgcgc
      181 cggacgacgg catcgtcacc aaggtggagc agctgcaggc cggcgacgcg atcgccgccg
      241 gcacgccggt gttcgcgctg atctccaccc atgacgtatg ggtcgaggcg aatttcaagg
      301 agagccagct cactcatatg caggccggcc agccggccga gatccgcatc gacacttatc
      361 ccggccggcg cttcagggcg cacgtagcca gcatcgcgcc gggcaccggc tcgcagttct
      421 ccgcgctgcc ggcggaaaac gccaccggca actgggtcaa ggtagtgcag cgcctgccgg
      481 tgcggctgga gctggagaac gcgccggcgg aactgccgct gcacaccggc ctgtcggccg
      541 atgtcgaggt cgacacgcag cccaagtagc gcatgcacac gcacgacgcc agcgggcgtc
      601 cgctgaatcg catgatgatc acctgcacgg tgatgctcac ctcgatcctg cagacgctcg
      661 acaacaccat cgccaacgtc gccctgccgc acatgcaggg cacgctgtcg gccacgcagg
      721 accagatgac ctgggtgctg acctcctaca tcgtcgccgc cgcggtgatg acgccgctca
      781 ccggctggct ggccggcgcc atcggccgca agcgcctgtt cctcgcgtcg gtggcgggct
      841 tcaccgtcac ctcgatgctg tgcgggctgg cgcagtcgct gccgcagatc gtgctgttcc
      901 gcttcctgca gggcatggcc ggcgccgcgc tggtgccgat gtcgcaggca gtgctgttcg
      961 acatcaaccc gcccgagcgc cacggcaagg cgatggcggc gtggggccag ggcgtgctgc
     1021 tcgggccgat gatcggcccc attctcggcg gctggctgac cgacaactac tcgtggcgct
     1081 gggtgttcta catcaacctg ccgctgggca tcctcgcctt ccttggcgtg ctggcctacc
     1141 tgccggaggg cgagaagcgc cgcacgcgct tcgatttctt cggcttcgcg ctgctgtcca
     1201 tattcatcgc cgcgctgcag ctggtgctgg accgcggccc gctgcaggac tggttcggtt
     1261 ccaccgagat ctggatcgag gccgccgccg ccgggctggc gctgtacctt ttcgccgtgc
     1321 acagcgccac cagccgcgag ccgttcttcc gtccggctct gttcaaggac cgcaacttcc
     1381 ttaccggcaa cgtgttcatc ttcgtggtcg gcatcgtgct gttcgccacg ctggcgctga
     1441 tcccgccgat gctgcaggag ctgatgggct acccggtgta ccaggccggc ctgctcaccg
     1501 cgccgcgcag catcggttcg ctcggcgcca tgctcatcgc cggccgcgtg gtgggcaagg
     1561 tcgattcgcg cctgatcatc gccggcggct ttgcgctgac ctgcttttcg ctgtggcaga
     1621 tgacgcgctt cgacctggcc atgaacggct ggccgatctt ctggagcggc gtattccagg
     1681 gcctgggcac cggcattgcc tacgtgccga tggcggcgct gaccttcgcc acgctcgatc
     1741 ccgggctgcg caacgaaggc accgcgctgt tctcgctgac gcgcaacatc ggcagcagca
     1801 tcggcatttc caccgtggca gcgctgctca cgcgcaacac gcagatcgtg cacagcacgc
     1861 tggccgagca cgtctcgccg tatggactgg cgctgcgcca tccgcagctg gctgcgcagc
     1921 tggcgcacgg cggcgcggcg gcggtcaacg ccg
//
LOCUS       DASZZC010000160         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_590815,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000160 DASZZC010000000
VERSION     DASZZC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590815"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /gene="rplE"
                     /locus_tag="VHE37_03620"
     CDS             <1..328
                     /gene="rplE"
                     /locus_tag="VHE37_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924186.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HVT34643.1"
                     /translation="KAIATFKIRKDLPIGAKVTLRGDQMYEFVDRLITMALPRVRDFR
                     GINPKSFDGRGNYAMGMKEHVVFLEIDYDKTETVWGMDIVFNTSAKTDEEARALLEGF
                     QFPFTN"
     gene            342..647
                     /gene="rpsN"
                     /locus_tag="VHE37_03625"
     CDS             342..647
                     /gene="rpsN"
                     /locus_tag="VHE37_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HVT34644.1"
                     /translation="MAKTSQINRNKKREKLVAQYAARRAALKAKADDMKVPAEERFEA
                     RLKLAKLPRNSSKTRIHHRCELSGRPKGYYRKVKMSRIALRSLSNFGQIPGMTKASW"
     gene            657..1049
                     /gene="rpsH"
                     /locus_tag="VHE37_03630"
     CDS             657..1049
                     /gene="rpsH"
                     /locus_tag="VHE37_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HVT34645.1"
                     /translation="MIIDPIGDLLSRIRNGQLRRKAKISSPNSRLRIRLLDVLQAEGF
                     IRGYAEVEVKGHKELEIELKYHEGEPVIRELKRVSTPGRRVYSSVSDLKPHRQGLGVS
                     ILSTPQGVMTDTSAREKNVGGEVLCQVF"
     gene            1059..1592
                     /gene="rplF"
                     /locus_tag="VHE37_03635"
     CDS             1059..1592
                     /gene="rplF"
                     /locus_tag="VHE37_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HVT34646.1"
                     /translation="MSRIGKKPVALPKGVIASVDGQTVKVKGPKGELSVKLAPEVSVS
                     ADEHGITVAPRKDAEKASQMWGLSRTLVNNLVTGVTQGFTQKLEIQGVGYRAAVQGKT
                     LNLQLGFSHDVPYAIPPGITIVTEKPTMISVSGIDKQLVGQVAAEIRSWRPPEPYKGK
                     GVRYEGEYVRRKEGKKK"
     gene            1592..>1725
                     /locus_tag="VHE37_03640"
     CDS             1592..>1725
                     /locus_tag="VHE37_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HVT34647.1"
                     /translation="MTQDLFKKRQSRNRYRLASHGSRPRLSIFRSGKHIYAQVIDDAR
                     A"
BASE COUNT          354 a          563 c          525 g          283 t
ORIGIN      
        1 gaaggcgatc gcgaccttca agatccgcaa ggatctgccg atcggcgcca aggtcaccct
       61 gcgcggcgac cagatgtacg aattcgtcga ccggctgatc accatggcgc tgccgcgcgt
      121 gcgcgacttc cgcggcatca atcccaagtc cttcgacggc cgcggcaact atgccatggg
      181 catgaaggag catgtcgtgt tcctggaaat cgactacgac aagaccgaaa ccgtgtgggg
      241 catggatatc gtgttcaaca cgtccgcgaa gaccgacgag gaagcccgcg cgcttctcga
      301 aggcttccag ttccccttta cgaattgagg cgctgatccc aatggcgaag acttcccaga
      361 tcaaccgcaa caagaagcgt gaaaagctgg tggcccagta tgccgccagg cgcgccgctc
      421 tcaaggccaa ggcggacgat atgaaggtgc cggccgagga gcgcttcgag gcgcgcctca
      481 agctggccaa gctgccgcgc aactcctcca agacgcgcat ccatcaccgc tgcgagctgt
      541 cgggccgccc caagggctat taccgcaagg tcaagatgtc ccgtatcgcg ctgcgttcgc
      601 tttccaattt cggccagatc cccggcatga ccaaggcgag ctggtaagga gcaaagatga
      661 tcatcgatcc gattggcgat ctgctttccc gcatccgcaa cggccagctg cgccgcaagg
      721 ccaagatcag ctcgcccaat tcgcgcctac gcatccggct gctcgatgtg ctgcaggccg
      781 aaggcttcat ccgcggctat gccgaggtcg aggtcaaggg ccacaaggag ctcgagatcg
      841 agctcaagta tcacgaaggc gaaccggtga tccgcgagct gaagcgcgtg tccacccccg
      901 gccgccgcgt ctattcctct gtcagcgact tgaagcccca tcgccagggt ctgggcgtgt
      961 ccattctctc cacgccgcag ggcgtgatga cggacacttc cgcccgcgag aagaatgtgg
     1021 gcggcgaagt cctctgccag gtcttttaag gagccgccat gtcccgtatc ggcaagaaac
     1081 ccgtcgcact gcccaagggc gttatcgcct cggtggatgg ccagaccgtc aaggtcaagg
     1141 gccccaaggg tgagctcagc gtcaagctgg cgccggaagt ctccgtctcg gcggacgagc
     1201 acggcatcac cgttgccccc cgcaaggacg cggaaaaggc ctcgcagatg tggggcctgt
     1261 cgcgcacgct ggtgaacaat ctcgtcaccg gcgtgaccca gggcttcacc cagaagctgg
     1321 aaatccaggg tgtcggctat cgcgccgcgg tgcagggcaa gaccctgaac ctgcagctcg
     1381 gcttcagcca tgacgtgccc tacgcgatcc cgccgggcat caccatcgtc accgagaagc
     1441 cgaccatgat ctcggtgtcg ggcatcgaca agcagctggt cggtcaggtt gcggccgaga
     1501 tccgctcctg gcgtccgccg gagccctata agggcaaggg cgtgcgctat gaaggcgagt
     1561 atgtccgccg caaggaaggc aagaagaagt aatgacccag gacctcttca agaagcgcca
     1621 gtcgcgcaat cgctaccggc tcgccagcca tggcagccgg ccgcgcctgt ccatcttccg
     1681 ctcgggcaag cacatctatg cccaggtgat cgacgatgcg cgcgc
//
LOCUS       DASZZC010000161         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1106724,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000161 DASZZC010000000
VERSION     DASZZC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106724"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VHE37_03645"
     CDS             <1..322
                     /locus_tag="VHE37_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34648.1"
                     /translation="VAVPVAVPNAAAAPPTDKGFWQKVVSYFESSDPDHPGLFDVMAS
                     SINIMQDRITRSRMAGDPAEITLLPQLEDFALMDFHRAAEAIEEGRALVRRHAEQIRA
                     WSVL"
     gene            413..667
                     /locus_tag="VHE37_03650"
     CDS             413..667
                     /locus_tag="VHE37_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HVT34649.1"
                     /translation="MNSDTLKGRWKELKGNVKQQWSKLTDDDMLAAEGGADKLSGAIQ
                     KRYGIARDEADRQIQNFLDSAGDAAERAGKRMRNESRPSA"
     gene            778..1152
                     /locus_tag="VHE37_03655"
     CDS             778..1152
                     /locus_tag="VHE37_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVT34650.1"
                     /translation="MKLRNFALIAALAAAPAFIVPAYAGPVADDMSQTGHDMASDAKG
                     AGHNIKEETKEAALTTKVKTALAKEKGLRSLKIHVDSEDGGVVTLTGKVKSTAQSEQA
                     ESATKAVEGVTTVHNNLEIKAD"
     gene            complement(1213..2091)
                     /locus_tag="VHE37_03660"
     CDS             complement(1213..2091)
                     /locus_tag="VHE37_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVT34651.1"
                     /translation="MAILTVEHTTTYRYARPVQFNEHRLMCRPRDSHDLRLLSTSLAI
                     TPPASVRWLHDVFGNSIAIAQFDGSAAELKFVSGFTAEHFPADAQDVAIEDYARALPF
                     NYAAEDVSDLGRTRERHYADAEHRVDRWAKSFVAREDGTLAVLLAMTQAIRHEFEYQR
                     REEMGTQTPVETLQQNSGSCRDFALLMMEAVRSLGLAARFVSGYLYDGSRAGVTGGGA
                     THAWVQVYLPGAGWIEFDPTNGIAGGRNLLRVAVARDAAQAAPLTGSYIGAPADYLGM
                     TVEVKVNVAATEIVAA"
     gene            2316..2846
                     /locus_tag="VHE37_03665"
     CDS             2316..2846
                     /locus_tag="VHE37_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT34652.1"
                     /translation="MHKQRSALLAVPVLLLAACSQPPPPAPAAPPAPPKPDLKAEEAT
                     LRQADKDLVAAIAAKNIDAVLGFYASDAVMMAPNEAAQSGDALKKGWTDLMALPEVAL
                     DFSATSIGIDPDAEMAYDVGTYTFGMASPKGKVDDHGNYVEVWRKTGGKWKIVVDAHS
                     SSVPLPQEAAPAAAKK"
BASE COUNT          538 a          952 c          944 g          418 t
ORIGIN      
        1 ggtggccgtg ccggtcgcgg tgcccaacgc cgcggcagcg ccgccgacgg acaagggctt
       61 ctggcagaag gtggtgagct acttcgagag cagcgatccc gatcacccgg gcctgttcga
      121 cgtgatggcc tccagcatca acatcatgca ggaccgcatc acgcgcagcc gcatggccgg
      181 cgatccggct gagatcaccc tgctgccgca gctcgaagac ttcgcgctga tggatttcca
      241 tcgcgcggcg gaggccatcg aggaaggccg cgcactggtg cggcggcacg ccgaacagat
      301 ccgcgcctgg agcgtgctgt aaaaaattcc acggagtcag cgcccgttca gggggtcaaa
      361 cccatactga ccgcgtggca tgaggccacg tttttccatg caaggaggcg acatgaacag
      421 cgatactctc aagggccgtt ggaaggaact caagggcaac gtcaagcagc agtggtccaa
      481 gctgaccgac gacgacatgc tcgcggccga aggcggcgcc gacaagcttt ccggcgccat
      541 ccagaagcgc tacggcattg cgcgcgacga ggcggatcgc cagatccaga acttcctcga
      601 cagcgccggc gatgccgccg aacgcgccgg caagcgcatg cgcaacgaat cgcggccgtc
      661 ggcctgaggc gcctgaccgc cgcgggcgcg gataagttcg ccgcacgttg cccgaaccca
      721 gccgtgccgc gggcggtctg ctagacgcaa cccattttgc ttcgacagga ggaacccatg
      781 aaactcagga actttgcatt gatcgccgcg ctggccgcgg ccccggcctt catcgtgccg
      841 gcctatgccg gcccggtggc cgacgacatg tcgcagaccg gccacgacat ggccagcgac
      901 gccaagggcg ccggccacaa catcaaggag gaaaccaagg aagccgcgct gaccaccaag
      961 gtgaagaccg cgctggccaa ggagaagggc ctgcgctcgc tgaagatcca cgtcgacagc
     1021 gaggacggcg gcgtggtcac gctgaccggc aaggtcaaga gcaccgcgca gtcggaacag
     1081 gccgaatccg ccaccaaggc ggtggaaggc gtgaccacgg tgcacaacaa cctcgagatc
     1141 aaggcggact gaaccccgct gcggtgaccg cagttgtgac tggcccgccg gtgcgcaagc
     1201 ccggcgggtt tttcatgccg ctacgatttc cgtagcagca acgttcacct tcacttccac
     1261 cgtcatgccc aggtaatccg ccggcgcccc gatgtagctg ccggtgagcg gcgcggcctg
     1321 cgcggcgtcg cgtgccaccg ccacgcgcag caggttgcgg ccgccggcga tgccgttggt
     1381 gggatcgaac tcgatccagc cggcgccggg caggtagacc tgcacccagg catgggtggc
     1441 gccgccgccg gtgacgccgg cgcgcgagcc gtcgtagaga tagccggaga cgaagcgcgc
     1501 ggcgaggccc aggctgcgca ccgcttccat catcagcagg gcgaagtcgc ggcagctgcc
     1561 ggaattctgc tgcagcgtct ccaccggcgt ctgcgtgccc atctcctcgc gccgctggta
     1621 ttcgaattcg tggcggatgg cctgggtcat cgccagcagc acggccagcg tgccgtcctc
     1681 gcgcgcgacg aagctcttgg cccagcggtc gacgcgatgc tccgcgtcgg cgtagtggcg
     1741 ctcgcgcgtg cggcccaggt ccgacacgtc ttccgccgcg tagttgaacg gcagggcgcg
     1801 cgcgtagtcc tcgatcgcca cgtcctgcgc gtccgccggg aaatgctcgg cggtaaagcc
     1861 gctgacgaat ttcagctcgg ccgcgctgcc gtcgaattgc gcgatggcga tggaattgcc
     1921 gaacacgtcg tgcagccagc gcacgctggc cggcggcgtg atggcgagcg aggtggagag
     1981 caggcgcagg tcatggctgt cgcgcgggcg gcacatcagg cggtgctcgt tgaactgcac
     2041 cggccgcgcg tagcggtagg tggtggtgtg ctcgacggtg agtatggcca tgatcggtgc
     2101 tcttgctctt gtgacaattg gagccgcaac cacccggctg gttcgcgcat gcagtgccca
     2161 ccgcaagctg cttttgactg ttataaaaaa tattacaaat tttaaaacta acaataaata
     2221 ataacactcc tttgcagggt gcggtccagg tttcccggcg cagcgcagcg ctacactgcg
     2281 ccgcgccgca ccggcaccaa ccagggagtc ttcacatgca caagcaacgc tccgcgctgt
     2341 tggcagttcc cgtactcctg ctcgccgcct gcagccagcc gccaccgccg gcacccgccg
     2401 caccgccggc gccgccgaaa cccgacctga aagcggagga ggccacgctg cgccaggccg
     2461 acaaggacct ggtcgccgcc atcgccgcca agaacatcga tgcggtgctc ggcttctacg
     2521 ccagcgatgc ggtgatgatg gcgcccaacg aagcggcgca gagcggcgat gcgctgaaga
     2581 agggctggac cgacctgatg gccctgcccg aagtggcgct ggatttctcc gccaccagca
     2641 tcggcatcga ccccgatgcc gagatggcct acgacgtcgg cacctacacc ttcggcatgg
     2701 cctcgcccaa gggcaaggtc gatgaccacg gcaactacgt ggaggtgtgg cgcaagaccg
     2761 gcggcaagtg gaagatcgtg gtcgatgcgc acagcagcag cgtgccgctg ccgcaggaag
     2821 cggcgcccgc cgccgccaag aaataactgc ag
//
LOCUS       DASZZC010000162         7130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_847459,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000162 DASZZC010000000
VERSION     DASZZC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847459"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..6012
                     /locus_tag="VHE37_03670"
     CDS             <1..6012
                     /locus_tag="VHE37_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34653.1"
                     /translation="AAQAALSARNQRKKATLTYAKGYDGAFNSYRRPQVYTEDDADPT
                     LNILQAKNSLGQVLGNIVQYAAHPTGVGTGAGGDRGRAVHADYVIGLEDTIESATGGA
                     ATLFYNGPIADASPSGPTVGNDDYQRVRSRGTCLAQDALALLDPQQALCSFAELKQAQ
                     VTRTTLQPQLSVRNTTAVMPVTNPLFLAVALTESFNRYYDFLQLPVDQIPALGPQIAA
                     LSPNLPQVAPVAQSEVSRITVGGADQGLEIVTIPGEGTNTFGRYIRGLTANKNMMLLG
                     LTHNSFGYIIPEEEFSYVDPAINCVQGSSDPFCNEGFVFPFTGYEEFVSLGPLTAALL
                     RDEAYNPLFDLPATDPRNLPPSATACATDPTGRACLLSHVIANVSYIQRSDAQACRRS
                     LASQVPADQQAAAQAFCGLLDPDTPLHQPCVDAGGPQAVCDALGNGSAAPSDTTLLAP
                     ALDALLRGCDFLDTSNCLYPFPNDHFTVDAPAGSPQSAEAGGTGRRLSISFAATPHNS
                     AGKPVDPTEWNRNDGFSPGALITTYVPGISLENTYGLPTSQIGVVNAGLSLADDSPIM
                     VLEVPQAPESLTPQHALVWSELDANANLLLPAAAPIVGSASDNSIPFDQFNRPLNDGK
                     AALLIRPAKNLAEGRRYVVVLRKLKDANGTALTAQDGFAACRDKVSSLLPPVQQRCAE
                     LEANVFPVLAAAGVARDASLYLAWDFTVASTNNQVGRLRHMRDDAFATLAAHDGADCS
                     EAHEGSDCHAPAFTVDTVTDNPQQGIVKRIEGTITVPSYLVPADASQLEYPAVTQVVS
                     QLCGFMPDAAPSQLRDLCGTLRDGSGTVQGASLPPNRLAYTPSLNPADPQGSLYGNGL
                     PTRTGTMSTRYMCQIPAQASPDDPARPAIYGHGLLDSHVAVTYDGVPEVSREFNRMYC
                     AVDWYGFATGDLGNVITALVDLSNFAVVPDASQQGVLNFMFLGRLLRDAGGFASNPAF
                     QDSATGRPLFDRSEVFYDGNSQGGIMGGVVIAADKDIHRGVLGSLGMNYSTLLTRSRD
                     FDQYAGALYLSYTDPLDRQLLFSMIQMLWDRSENDGYAAHLTDNSAFGGQSKAVKLDP
                     NFGDQQVSLWTADVMARTMGIPYNADQTDRAAAILGENPRNPDVVPYLGLQPLDFSDP
                     AQAAGSAMIVWDSKHNGAYADIPPLNNTPPRTGPDPHDNSAKTTSGRCQKAHFLRTGG
                     QLIDITDLEFDPANNTCPAVPKAAPTVAVPQLSSYGTGVLGALAQFMHEANGAFMALV
                     QGDYTDAGIALQTAFANFSSNLGQVLGGGSASAAVLAQDAANNLSNPQVLASQLGADA
                     GALAGLDTDAISAATRATSANRNAIAVVLTGVQVPAWSVPAAQGVPYPYPSGATISGQ
                     LFDALGVIPGQVHDAHNGVMLYPVPGSPTGKPVERIAAYKWDGTQFVEIPVQVDEKFP
                     FFLANADSSFSVYSGTDEELTYAWDLERWQNTDDPANLCNAGYSKGMADPVAGLDDDD
                     EIAFMAQDSGAQAPQGARPADPDLDPAETLQMVALVDALDPQAQRYVYLGLKKDGRLP
                     KFAGQKVYVDYERDANADQWIDRTFFADSDPLKLGTSNTGYGPNRHGKVCGDGTPASA
                     RDSTDRFPRDGTRVTTDAYQWYASGRWMVREMHVARPNQSLPAGRAPGAGVKFGADLV
                     DRWKGRAFQQAPDSNISIVGFEDEQVNWEANSTLLGERCGPVRCMREVWGADSGTNVT
                     KTETFYRDAVAYRYHVRVHPIPPDGLYTSWDYNRSAMIPDANERVPGGRYYTILRPQG
                     VPIDGVNDDVGQVDSLTTLPVNGQCVSPDNNGLVPPNPDGHCPAFFDVADPTFNLPLA
                     FDNWEQVSGKGNLGSLVYTFELKGPTSLANPLVIPFYRDDACLDDGTGDDPVQRPYPG
                     EAQSDPRVRAGYVAAAGGDPNHNVDAQFVSLGCEQKQGAYGAHGVHYLVTHDSDNAFL
                     LGKPIDEVDGQQWQFAVPTPRPMNVAEPYANDIRVPLTGVAVPAP"
     gene            complement(6198..>7130)
                     /locus_tag="VHE37_03675"
     CDS             complement(6198..>7130)
                     /locus_tag="VHE37_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34654.1"
                     /translation="PGKMKIRYALSAVALAASAAAGAGSAIDFKAFMAQVPALPKNSA
                     AAAQAYTAERWDLLPEMPAAVTQLQKDLGAAQVQASMQSAGTPAASPIDPSQAQAMAA
                     RLQSMSQQEKIAYAMQMQAQMAPHPAMGVVSDNQGQAIKQISQEQQQLALDNQKTAAM
                     RVAHGQFLAAWDQRDAQLSTQNGRMENGEEGGNADFVCSEAERANRLKAVALHQKIAE
                     DELAQAAKISAEQRAYTEAAIARFQRISALAPQVGPMASMANTALQSSSSDATLAVSE
                     LAGFNAEAGRRAARWPHMAAQLPKLRVENCSTGG"
BASE COUNT         1155 a         2555 c         2313 g         1107 t
ORIGIN      
        1 gccgcgcagg cggcgctgag cgcgcgcaac cagcgcaaga aagcgacgct gacctacgcc
       61 aagggctacg acggcgcatt caattcctat cgccgtccgc aggtgtacac cgaggatgac
      121 gccgatccca cgctcaacat cctgcaggcg aagaacagcc tgggccaggt gctgggcaac
      181 atcgtgcagt acgccgcgca tccgaccggc gtgggcaccg gcgccggcgg cgaccgcggc
      241 cgcgcggtgc atgccgacta cgtgatcggc ctggaagaca ccatcgagtc cgccaccggc
      301 ggcgcggcca cgctgttcta caacggcccg attgccgatg cttcgcccag cggccccacc
      361 gtgggcaacg acgactacca gcgcgtgcgc tcgcgcggca cctgccttgc gcaggacgcg
      421 ctggccttgc tcgatccgca gcaggcgctg tgcagcttcg ccgagctgaa gcaggcgcag
      481 gtgacgcgca ccacgctgca gccgcagctg tcggtgcgca acaccacggc cgtcatgccg
      541 gtgaccaacc cgctgttcct ggcggtggcg ctgaccgaga gtttcaaccg ctactacgac
      601 ttcctgcagc tgccggtgga ccagatcccg gcgctcggcc cgcagatcgc cgcgctgtcg
      661 cccaacctgc cgcaggtggc gccggtggcg cagagtgagg tgtcgcgcat caccgtcggc
      721 ggcgccgacc agggcctgga gatcgtgacc attccgggcg aaggcaccaa caccttcggc
      781 cgctacatcc gcgggctcac cgccaacaag aacatgatgc tgctgggcct gacccacaac
      841 tcgttcggct acatcatccc cgaagaggaa ttcagctacg tcgaccccgc gatcaactgc
      901 gtgcagggca gcagcgatcc gttctgcaac gaaggctttg tcttcccctt caccggctac
      961 gaggaattcg tttcgctcgg gcccctcacc gcggcgctgc tgcgcgacga ggcctacaac
     1021 ccgctgttcg acctgccggc caccgatccg cgcaacctgc cgccgagcgc taccgcctgc
     1081 gccaccgatc cgaccggccg cgcctgcctg ctgtcgcatg tcatcgccaa cgtctcctac
     1141 atccagcgct ccgatgcgca ggcctgccgc cgcagcctgg cttcgcaggt gccggccgac
     1201 cagcaggccg ccgcgcaggc gttctgcggc ctgctcgatc cggacacgcc gctgcaccag
     1261 ccctgcgtcg atgcgggcgg accgcaggcg gtgtgcgatg cgctgggcaa cggcagcgcc
     1321 gcgccgtctg acaccacgct gctggcgccg gcgctcgacg cgctgctgcg cggctgcgac
     1381 ttcctcgaca ccagcaactg cctctatccg tttcccaacg accacttcac ggtggacgcg
     1441 ccggccggct cgccgcaatc ggccgaagcc ggcggcaccg gccgccgcct gagcatcagc
     1501 ttcgccgcca cgccgcacaa cagcgcgggc aagcccgtcg atcccaccga gtggaaccgc
     1561 aacgacggct tctcgcccgg cgcgctgatc accacctacg tgcccggcat ctcgctggag
     1621 aacacctacg gcctgccgac cagccagatc ggcgtggtca acgccgggct gtcgctggcc
     1681 gacgactcgc cgatcatggt gctggaagtg ccgcaggcgc cggaaagcct gacgccgcag
     1741 cacgcactgg tgtggtcgga actcgacgcc aacgccaacc tgctgctgcc cgcggccgcg
     1801 cccatcgtcg gctcggccag cgacaacagc attccgttcg accagttcaa ccgcccgctc
     1861 aacgacggca aggccgcgct gttgatccgc ccggcgaaga accttgccga aggccgccgc
     1921 tacgtggtgg tgctgcgcaa gctcaaggat gcgaacggca ccgcgctgac cgcgcaggat
     1981 ggcttcgccg cctgccgcga caaggtcagc agcctgctgc cgccggtgca gcagcgctgc
     2041 gccgagctgg aagccaacgt gttcccggtg ctggccgcgg ccggcgtggc gcgcgacgcc
     2101 agcctgtacc tggcctggga tttcaccgtg gcgtcgacca acaaccaggt cggccggctg
     2161 cgccacatgc gcgacgacgc cttcgccacg ctggctgcgc acgatggcgc ggactgcagc
     2221 gaggcgcatg aaggcagcga ctgccacgcg ccggcgttca ctgtcgacac cgtcaccgac
     2281 aatccgcagc agggcatcgt caagcgcatc gagggcacca tcaccgtgcc gagctatctg
     2341 gtcccggcgg atgcctcgca actggaatac ccggcggtga cgcaggtggt gtcgcagctg
     2401 tgcggcttca tgcccgatgc ggcgccatcg cagctgcgcg acctctgcgg cacgctgcgc
     2461 gatggcagcg gcacggtgca gggtgccagc ctgccgccga accggctcgc gtacacgccg
     2521 tcgctcaatc cggccgatcc gcagggcagc ctctacggca acggcctgcc gacgcgcacc
     2581 ggcaccatga gcacgcgcta catgtgccag atcccggcgc aggcctcgcc cgacgacccg
     2641 gcgcgcccgg ccatctacgg ccacggcctg ctcgattctc acgtggccgt cacctacgac
     2701 ggcgtgccgg aagtgtcgcg cgagttcaac cgcatgtact gcgcggtcga ctggtacggc
     2761 ttcgccaccg gcgacctggg caacgtgatc accgcgctgg tcgacctgtc gaacttcgcg
     2821 gtggtgcccg acgcctcgca gcagggcgtg ctcaacttca tgttcctcgg acgcctgctg
     2881 cgcgacgccg gcggcttcgc ctcgaacccg gcgttccagg attcggccac cggccgcccg
     2941 ctgttcgacc gcagcgaggt gttctacgac ggcaacagcc agggcggcat catgggcggc
     3001 gtggtcatcg ccgccgacaa ggacattcac cgcggcgtgc tcggttcgct gggcatgaac
     3061 tactcgacgc tgctcacgcg ctcgcgcgat ttcgaccagt acgccggtgc gctgtatctc
     3121 tcgtacaccg atccgctgga tcgccagctg ctgttctcga tgatccagat gctgtgggac
     3181 cgctcggaga acgacggcta cgccgcgcac ctgaccgaca acagcgcgtt cggcggccag
     3241 tcgaaagcgg tgaagctcga cccgaacttc ggcgaccagc aggtgagcct gtggaccgcc
     3301 gacgtgatgg cgcggaccat gggcattccg tacaacgcgg accagaccga tcgcgccgcc
     3361 gccatcctgg gcgagaaccc gcgcaatccc gacgtggtgc cgtatctcgg gctgcagccg
     3421 ctggacttca gcgatccggc gcaggccgcc ggcagcgcga tgattgtgtg ggactccaag
     3481 cacaacggcg cctacgccga catcccgccg ctgaacaaca cgccgccgcg caccgggccc
     3541 gatccgcacg acaactcggc caagaccacc tcgggccgct gccagaaagc gcacttcctg
     3601 cgcaccggcg gccagctgat cgacatcacc gatctcgagt tcgacccggc caacaacacc
     3661 tgcccggcgg tgccgaaggc ggcgccgacg gtggcggtgc cgcagctgtc gagctacggc
     3721 accggtgtgc tcggcgcgct tgcgcagttc atgcacgagg ccaacggcgc gttcatggcg
     3781 ctggtgcagg gcgactacac cgatgccggc atcgcgctgc agacggcttt cgccaacttc
     3841 agctcgaacc tcggccaggt gctgggcggc ggcagcgcca gcgccgcggt gctggcgcag
     3901 gacgctgcga acaatctttc caacccgcag gtgctggcct cgcagctggg cgctgatgcc
     3961 ggcgcgctcg ccgggctcga caccgacgcg atcagcgccg ccacgcgcgc cacttcggcc
     4021 aaccgcaacg ccatcgccgt ggtgctgacc ggcgtgcagg tgccggcgtg gtcggtgccg
     4081 gcggcgcagg gcgtgccgta tccgtacccc tcgggcgcca ccatcagcgg ccagctgttc
     4141 gacgcgctcg gtgtcattcc cggccaggtg cacgacgcgc acaacggcgt gatgctctac
     4201 ccggtgccgg gttcgccgac cggcaagccg gtggagcgca tcgccgcgta caagtgggat
     4261 ggcacgcagt tcgtggaaat cccggtgcag gtcgacgaga aattcccgtt cttcctggcc
     4321 aatgcggact cgagcttctc ggtgtactcg ggcaccgacg aggagctgac ctacgcctgg
     4381 gacctggagc gctggcagaa caccgacgat ccggccaacc tgtgcaatgc cggctacagc
     4441 aagggcatgg ccgatccggt ggcgggtctg gacgatgacg acgagatcgc cttcatggct
     4501 caggacagcg gtgcgcaggc gccgcagggt gcgcgcccgg ccgatcccga tctcgatccg
     4561 gccgagacat tgcagatggt ggcgctggtc gacgcactcg atccgcaggc gcagcgctat
     4621 gtctatctcg gcctgaagaa ggacggcagg ctgccgaagt tcgccgggca gaaggtctac
     4681 gtcgattatg agcgcgacgc caacgcggac cagtggatcg accgcacttt cttcgccgac
     4741 agcgatccgc tgaagctcgg caccagcaac accggctacg gtcccaaccg ccacggcaag
     4801 gtttgcggcg acggcacgcc ggccagcgcg cgcgattcca ccgaccgctt cccgcgcgac
     4861 ggcacgcgcg tgaccaccga tgcctaccag tggtacgcca gcgggcgctg gatggtgcgc
     4921 gagatgcacg tggccaggcc caaccagtcg ctgccggccg gccgcgcgcc gggcgcgggc
     4981 gtcaaattcg gcgccgacct ggtcgaccgc tggaaaggcc gcgccttcca gcaggcgccc
     5041 gattccaaca tctcgatcgt cggcttcgag gacgagcagg tgaactggga agccaactcc
     5101 acgctgctgg gcgagcgctg cggcccggtg cgctgcatgc gcgaagtgtg gggtgcggac
     5161 tcgggcacca atgtcaccaa gaccgagacc ttctaccgcg acgcggtggc ctaccgctac
     5221 cacgtgcgcg tgcacccgat tccgccggat ggcctctaca cctcgtggga ctacaaccgc
     5281 agcgcgatga tcccggatgc gaacgagcgc gtgccgggcg gccgctacta caccatcctg
     5341 cggccgcagg gcgtgccgat cgacggcgtc aacgacgacg tgggccaggt cgattcgctg
     5401 accacgctgc cggtcaacgg ccagtgcgtg tcgccggaca acaacggcct ggtgccaccg
     5461 aacccggacg gacattgccc ggcgttcttc gacgtggccg acccgacctt caacctgccg
     5521 ctggcgttcg acaactggga gcaggtgtcg ggcaagggca acctaggttc gctggtctat
     5581 accttcgagc tcaaggggcc gacctcgctg gccaatccgc tggtcatccc gttctaccgc
     5641 gacgacgcct gcctggacga cggcaccggc gacgacccgg tgcagcgccc ttacccgggc
     5701 gaggcgcaga gcgatccgcg cgtgcgcgcc ggttacgtgg cggcggctgg cggcgatccc
     5761 aaccacaacg tcgacgcgca gttcgtcagc ctggggtgcg agcagaagca gggcgcgtat
     5821 ggcgcgcacg gcgtgcatta cctggtgacg cacgactccg acaatgcctt cctgctgggc
     5881 aagccgatcg acgaagtgga tggccagcaa tggcagttcg cggtgccgac gccgcggccg
     5941 atgaacgtgg ccgagcctta cgccaacgac atccgcgtgc cgctcaccgg cgtggcggtg
     6001 cccgcgccgt aaaaagaatc aacgtatttt ttcaggttct cttcaagccc cggcgagtct
     6061 tgccggggtt attttttgcc tgcggcaagc tccaccgcac cgccatttct gatgttataa
     6121 attattttat gtttatatat ttaatcgata ttttataaca gacaaaacag cgatgcggcg
     6181 tggcgttttt tccggcatca accaccggtg ctgcagttct ccacgcgcag cttgggcagt
     6241 tgcgccgcca tgtgcggcca gcgcgcggcg cggcgtcctg cctccgcgtt gaaaccggcg
     6301 agctcgctga ccgccagcgt ggcatcggag cttgacgact gcagcgcggt gttggccatg
     6361 cttgccattg gccctacctg cggcgccagc gcggagatgc gctggaagcg cgcgatggca
     6421 gcttcggtgt acgcacgctg ctcggcggaa atttttgcgg cctgcgccag ttcgtcctcg
     6481 gcaatcttct ggtgcagggc caccgccttg agccggttgg cgcgctcggc ctcgctgcag
     6541 acgaaatcgg cattgccgcc ttcctcgccg ttctccatgc ggccattctg cgtggacagc
     6601 tgcgcatcgc gctggtccca cgcggccagg aactggccgt gcgccacgcg catcgccgcg
     6661 gtcttctggt tgtcgagcgc cagctgctgc tgctcctgcg agatctgctt gatcgcctgt
     6721 ccctggttgt cgctgaccac gcccatggcg ggatgcggcg ccatctgcgc ctgcatctgc
     6781 atggcgtagg cgatcttctc ctgctggctc atcgactgca gcctggccgc catggcctgc
     6841 gcctgcgatg gatcgatggg cgaagcggcc ggcgtgccgg ccgactgcat gctggcctgc
     6901 acctgcgccg cgccgagatc cttctgcagc tgcgtcaccg cggccggcat ctcgggcagc
     6961 aggtcccagc gctcggccgt atacgcctgc gctgcagccg cgctgttctt cggcagcgcc
     7021 ggcacctgcg ccatgaacgc cttgaagtcg atggcgctac cggcgccggc agcggcgctg
     7081 gcagcaagcg caacagcgga aagtgcgtaa cggattttca tcttgcctgg
//
LOCUS       DASZZC010000163         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_654633,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000163 DASZZC010000000
VERSION     DASZZC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654633"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VHE37_03680"
     CDS             <1..467
                     /locus_tag="VHE37_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT34655.1"
                     /translation="HDGTCAEGTSPGGYAGIDAFLREWVPKIVNSPAFQQDGMLLVTF
                     DESDDNPDDGNAACCNEPSGPNTTAPGAGNIGGNPSFLLYPGGGRIGGVIVSPYTRPG
                     TVSDVPYNHYSMLRSVANMFGAAHLGYAGEPGLVPFGSDIYSRGTAPADLGN"
     gene            complement(477..>2383)
                     /locus_tag="VHE37_03685"
     CDS             complement(477..>2383)
                     /locus_tag="VHE37_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HVT34656.1"
                     /translation="YSGLAFRATEPDVLLMAGHSFSTGSDGAASIYAVGVTRDAQGHI
                     NGFAPRARLVSAAPGVDGDATTGLAYNGVGSGISIGPGSRVVYISPRDSTLGVLAAGQ
                     KTPAQLIDLRTLEPTALPGIEAMLRLPQTFSGAGRLKLGNSSTGDLYDINLAPDGSGS
                     GDLKVTSVAAAGTLTGIGSFFHQDTPDPVTGDPRSHTNVNQLTGLLAVAHGSPQFDHD
                     ALLSVSCDYNTGEDSNLVAYDVDSAGNPVAGSDRVVVSQLCATGATVDPVSGDLLFTN
                     FYGFPSLTVIRGFSAPLPSAQFVRNSFNVAESGGSATITVERIGDNSSALAVDYSTAA
                     GTAASGMNYQDVSGTLNWSAGDAAKKTFTVPVMSDHLYNGSLTVALTLAPENGGTPSQ
                     ATLHIANEDPRPVVTLTSADRDVDESAGAIDITVAVSPLSGQSIKLPLRFSGTAQRNR
                     DFTLDQNLSGQPVNYIVIPPGSDGSGLTLPIHITDDVMHEDDETLTVTLQPTFNADTG
                     SVTSNTITIHDNDALPSAGFAAASSTVKEGSRGGGVAVPAALIRLSAPSSTDVSVPVT
                     LVGGGLDPRITLVSPVIIPAGRLSAYAVLVADDGAKEAPVQLTLSIGAPTGATQGSQS
                     TQVLTVKDLD"
BASE COUNT          356 a          787 c          848 g          392 t
ORIGIN      
        1 gccacgacgg cacctgcgcc gagggcacct cgccgggcgg ctatgccggc atcgatgcct
       61 tcctgcgcga gtgggtgccc aagatcgtca actcaccggc cttccagcag gacggcatgc
      121 tgctggtgac tttcgacgaa tcggacgaca accccgacga cggcaacgcc gcctgctgca
      181 acgagcccag cggccccaac accacggcac cgggcgccgg caacatcggc ggcaatccca
      241 gcttcctgct gtacccgggg ggcggccgca tcggcggcgt gatcgtttcg ccgtacacgc
      301 gcccgggtac ggtgagcgac gtgccttata accactactc gatgctgcgc agcgtggcca
      361 acatgttcgg agcggcgcac ctgggctacg cgggcgagcc gggactggtc ccgtttggca
      421 gcgacatcta cagccgcggc acggcgccgg cagacctggg caactgagcg ccgcgctcag
      481 tcgagatcct tcaccgtcag cacttgcgtc gactggctgc cctgggtggc gccggtgggc
      541 gcgccgatcg acagcgtcag ctggaccggc gcttccttcg cgccatcgtc ggccaccagc
      601 accgcgtagg ccgacagcct gcccgccggg atgatcaccg ggctgaccag cgtgatgcgc
      661 ggatcgagtc cgccgcccac cagcgtcacc ggcacgctca cgtcggtgct gctgggcgcc
      721 gacagcctga tcagcgcggc cggcacggcg acgccgccgc cgcgcgaacc ttccttcacc
      781 gtgctgctgg ctgcggcgaa gccggcgctg ggcaatgcat cgttgtcgtg aatggtgatg
      841 gtgtttgagg tgacgctgcc ggtgtcggca ttgaacgtcg gctgcagcgt gacggtgagc
      901 gtctcgtcgt cttcatgcat gacatcgtcg gtgatgtgga tcggcagcgt caggccgctg
      961 ccgtcgctgc ccggcggaat cacgatgtag ttcaccggct ggccggacag gttctggtcc
     1021 agggtgaaat ccctgttccg ctgcgcagtg cccgagaagc gcaagggcag cttgattgac
     1081 tgccccgaca gcgggctgac tgccaccgtg atgtcgatgg cgcccgccga ttcatcgacg
     1141 tcgcggtcgg ccgacgtgag ggtgaccacc ggcctgggat cttcattggc gatatgcagc
     1201 gtggcctgcg agggcgtgcc gccgttttcc ggcgccagcg tcaacgccac cgtgagcgag
     1261 ccgttgtaca ggtggtcgct catcaccggc acggtgaagg ttttcttcgc ggcatcgccg
     1321 gcgctccagt tcagcgtgcc gctgacatcc tggtagttca tgccgctggc ggcagtgccg
     1381 gccgcggtcg agtaatcgac ggccagggcc gaggagttgt cgccgatgcg ctcgacggtg
     1441 atcgtcgcgc tgccgccgga ttcggcgacg ttgaacgaat tgcgcacgaa ctgcgcagag
     1501 ggcagcggcg cgctgaagcc gcggatcacc gtcaggctgg ggaaaccgta gaagttggtg
     1561 aacagcaggt cgccgctgac cgggtccacc gtggcgccgg tggcgcacag ctggctgacc
     1621 accacgcggt cgctgccggc caccgggttg cccgcgctgt ccacgtcgta cgcgaccagg
     1681 ttgctgtcct cgccggtgtt gtagtcgcaa ctcaccgaga gcagcgcatc gtgatcgaac
     1741 tgcgggctgc cgtgcgcaac ggcgagcagt ccggtgagct ggttgacgtt ggtgtgcgaa
     1801 cgcggatcgc cggtgacggg atcgggcgtg tcctggtgga agaacgagcc gatgccggtc
     1861 agcgtgccgg ccgcggccac gctggtcacc ttgagatcgc cgctgccgct gccgtcgggc
     1921 gccaggttga tgtcgtacag gtcgccggtg ctggagttgc ccagcttcag ccggccggcg
     1981 ccggagaagg tctgcggcag gcgcagcatg gcttcgatgc cgggcagcgc ggtcggttcc
     2041 agggtgcgca ggtcgatcag ctgcgccggc gttttctggc cggcggcgag caccccgagc
     2101 gtgctgtcgc gcggactgat gtagaccacg cgactgccgg ggccgattga aatgccgctg
     2161 ccgacgccgt tgtaggccag cccggtcgtt gcatcaccat cgacgccggg tgccgcggac
     2221 accagccgtg cgcgtggcgc gaaaccgttg atgtgtccct gcgcatcgcg ggtgacgccg
     2281 acggcgtaga tcgaggccgc gccatcgctg ccggtggaga acgagtggcc cgccatcagc
     2341 aacacatccg gctcggtcgc gcggaaggcc aggccggagt agg
//
LOCUS       DASZZC010000164         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_464259,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000164 DASZZC010000000
VERSION     DASZZC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464259"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VHE37_03690"
     CDS             <1..474
                     /locus_tag="VHE37_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HVT34657.1"
                     /translation="WASLHHPFTAPKVQSADEIRNNPGGVLSQGYDIVLNGIELGGGS
                     VRIHRPEVQQAVFDLLGIGPQQQQEKFGFLLEALSYGAPPHGGLAIGIDRMVMLMAGG
                     QSIREVIAFPKTQTAHDPLTSAPSNVDARQLRELSIRSTVATKSEALQEPKGNAS"
     gene            471..1889
                     /locus_tag="VHE37_03695"
     CDS             471..1889
                     /locus_tag="VHE37_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34658.1"
                     /translation="MRLPYALRAAVAAAAVLLASCGHISQGNQLVSIRFTSATNALVP
                     QTTRELFQCFAQQLTLIGEFTDHTLGNFTTRGAVYSSSDPTKVQVSNGDIPAPGGTGF
                     YARGVVIPVEPSGPVVITASYATLTASIQVSVSPIDPIKVTPADAYMAPGSNLRFTAT
                     TMVDGNPLDVSTSGAWVVDAPQSGIASINPSSGVATAGSAPGDITARLSVPLCQGSAT
                     DPTASATATIHVAAPDHLEVTRQFPGQPILFATSPNSYATTDAITTLAVFPGGQTEDL
                     STQAVLQLTPPDSGTLLVQNTNLLRSVAEGTPVTVSSFYNSAGTLLRADLPQNNTAGQ
                     PLEQISVRNATLDSIAISPGDQNPTIPTFGTYPFHAIGTFTGDDGLTYTQDITRHVAW
                     SSSDATVVMSNIYSGSLLLTGGNGTATSTADQGVCAQITADTGVPATDGSGNILATTT
                     LAVVPGVAAQPGSPANSPCRLP"
     gene            1956..>2177
                     /gene="nadA"
                     /locus_tag="VHE37_03700"
     CDS             1956..>2177
                     /gene="nadA"
                     /locus_tag="VHE37_03700"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HVT34659.1"
                     /translation="MATVIPLDQIRKIDQFNLLDDSECDARIVAAKNILGKKLLILGH
                     HYQRDEVFKHADFSGDSLKLSQQAANSQAE"
BASE COUNT          393 a          799 c          669 g          316 t
ORIGIN      
        1 tgggcctcgc tgcatcatcc gttcaccgcg ccgaaagtgc agagcgccga cgagatccgg
       61 aacaatccgg gcggcgtgct gtcgcagggc tatgacatcg tcctcaacgg catcgagctc
      121 ggcggcggca gcgtgcgcat ccatcgcccg gaagtgcagc aggcggtgtt cgacctgctg
      181 ggcatcggtc cgcaacagca gcaggagaag ttcggcttcc tgctggaagc gctgtcgtac
      241 ggcgcgccgc cgcacggcgg cctggccatc ggcatcgacc gcatggtcat gctgatggcg
      301 ggcgggcagt cgatccgcga agtcatcgcc ttccccaaga cgcagaccgc gcacgatccc
      361 ctgaccagcg caccgagcaa tgtcgatgcg cgccagctgc gtgagctgtc gatccgctcg
      421 accgttgcta cgaaatccga agcgctgcag gaacccaaag gaaacgcctc gtgagattgc
      481 cttatgcgct gcgcgccgcc gtggcggctg ccgctgtcct gcttgcgtcc tgcggccaca
      541 tcagccaggg caaccagctg gtgtcgattc gcttcacctc ggccaccaat gccctggtgc
      601 cgcagaccac gcgcgagctg ttccagtgct tcgcgcagca gctgacgctg atcggcgagt
      661 tcaccgatca cacgctgggc aacttcacca cgcgcggcgc cgtctacagc agcagcgatc
      721 cgaccaaggt gcaggtatcc aacggcgata tcccggcacc gggcggcacc ggcttctatg
      781 cgcgcggcgt ggtcatcccg gtggagccga gcgggccggt ggtcatcacc gccagctatg
      841 ccacgctgac cgcgagcatc caggtgagcg tgagcccgat cgatccgatc aaggtcacgc
      901 cggccgatgc ctacatggcg ccaggctcca acctgcgctt caccgcgacc accatggtcg
      961 atggcaaccc gctcgacgtc agcacctccg gcgcctgggt ggtcgatgcg ccgcagtcgg
     1021 gcatcgccag catcaacccc agcagcggcg ttgccaccgc cggcagcgcg cccggcgaca
     1081 tcaccgcgcg cctgtcggtg ccgctgtgcc agggctcggc caccgatccc accgcgagcg
     1141 ccaccgccac catccatgtg gcggcgccgg atcacctcga ggtgacgcgg cagttcccgg
     1201 gccagccgat cctgtttgcg accagcccga acagctacgc caccaccgac gccatcacca
     1261 cgctggcggt gttccccggc ggccagaccg aggacctgtc gacccaggcg gtcctgcagc
     1321 tgacgccgcc cgacagcggc acactgctgg tgcagaacac caacctgctg cgctcggtgg
     1381 ccgaaggcac gccagtgacc gtcagcagct tctacaacag tgccggcacg ctgttgcgcg
     1441 ccgacctgcc gcagaacaac acagcgggac agccgctcga acagatcagc gtgcgcaacg
     1501 ccacgctgga ttcgatcgcc atcagccccg gcgaccagaa cccgaccatt ccgaccttcg
     1561 gcacctatcc gttccatgcc atcggcacgt tcaccggcga tgacggtctg acctacacgc
     1621 aggacatcac ccgccacgtc gcctggagtt cgagcgacgc cactgtggta atgagcaaca
     1681 tctacagcgg cagcctgctg ctgaccggtg gcaacggcac cgcgacctcg accgccgacc
     1741 agggcgtgtg cgcgcagatc accgccgaca ccggcgtgcc ggccaccgac ggcagcggca
     1801 acatcctggc gacaacgaca ctggcggtgg tccccggcgt ggccgcgcag ccgggctcgc
     1861 cggccaactc gccctgccgc ctgccgtaag cagcggcgcc ttgcagtaaa atagcgcgcc
     1921 gtttcgccgc gcgttgcgcg cgccggaggt gatccatggc cactgtcatt cccctcgacc
     1981 agatccgcaa gatcgaccag ttcaacctgc tggacgacag cgagtgcgac gcgcgtatcg
     2041 tcgcggcgaa aaacatcctc ggcaagaaac tgctgatcct gggccatcac taccagcgcg
     2101 acgaggtgtt caagcacgcc gatttctccg gcgattcgct caagctgtcg cagcaggcgg
     2161 ccaacagcca ggccgag
//
LOCUS       DASZZC010000165         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_976283,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000165 DASZZC010000000
VERSION     DASZZC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976283"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..566
                     /locus_tag="VHE37_03705"
     CDS             156..566
                     /locus_tag="VHE37_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT34660.1"
                     /translation="MLSRRHNESEGHTGIDLAPMLDFVLNLLIFFIITTSFVKQTGVN
                     VTRQEAVTAEHKENGNILIAIRPNGEIWMDKRAVDLRDIRGQIERLHVERPDDTVVIV
                     ADKLAAAGVLTQVMDQVKMGGVQDVSIAALPQGG"
     gene            570..1211
                     /locus_tag="VHE37_03710"
     CDS             570..1211
                     /locus_tag="VHE37_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT34661.1"
                     /translation="MFLIQSPFARPLLYAGIFALSLFWLLHALINRSHSVDKSEALQT
                     VDFVRLRKDTDIESRIRQKPPKPQPPKEPPPPKMQTVAPQERPNQQRLPFNIPNLNTG
                     LNFGGGPFIGNLAAAAEAGGYSELIPLVALQPQYPRQAARDGIEGWVDVEITIGPDGS
                     VKSARAKDAKPRGVFEAAAISAALKSRFRPKMVEGKGVEATGIRRYSFNLGKE"
     gene            1208..2656
                     /locus_tag="VHE37_03715"
     CDS             1208..2656
                     /locus_tag="VHE37_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT34662.1"
                     /translation="MMRLSIKLRLLPLLLALVCALSAAVMPAPAMADDQPAPAKKERK
                     ATGSMSEGTYHALEKVYKLMGDNKNAEALAALQTLQDKVSGVYEKCIVLQTIGYVYAN
                     QNNYKKAIEVFEQALALDALPQQPYEQLLYNIAQMDVATEQYDKGIAMIERYFAEATF
                     AKPPPDSHILAASAYAEKKRFADALKQVDIALAEAKAPKEPWLQLKLALHYELKQYPQ
                     CAEVLLQLIAIAPTKKDYWKQLSSMFFEIKEDKESLAVLALADRQGYLTEDHEYRNLA
                     NVYMLLEIPYKAGQVLQRGFDRKILKEDEKSLTMLADAWQLAREGDKAAEALRRAADA
                     TGKGDLYLRLAGVYAEDEKWKKSLEAVNLALSKGVTKTGPALLLAGQVAYHAGDKAAA
                     RRDLNKAMEHEDVRKPAHDWLQYMEQMEGEPAVVANASSGADDQQQSAGDNNASAPAP
                     SAPAAPAAPAAPAKTAAPPSPPKPAPPPARKK"
BASE COUNT          546 a          849 c          863 g          417 t
ORIGIN      
        1 acatcgagcg catgcacgcg ctcaagcccg acgatgccgt gctggtcatc gccgacaagg
       61 aagccccgag tggcatcgtg gtccaggtac tcgaccaggt gcgcgagggt ggtgtactca
      121 attcgtcact cacttcaggc tcgctcaagt aagccatgct gtcgcgccgc cataacgaaa
      181 gcgaaggcca caccggcatc gacctcgcgc cgatgctcga cttcgtgctc aatctgctga
      241 tcttcttcat catcaccacc tcgttcgtca agcagaccgg cgtgaatgtc acgcgccagg
      301 aagcggtcac ggccgagcac aaggaaaacg gcaacatcct catcgccatc cgtcccaacg
      361 gcgaaatctg gatggacaag cgcgccgtcg atctgcgcga catccgcggg cagatcgagc
      421 gcctgcatgt cgagcgtcct gacgacacgg tggtcatcgt ggccgacaag ctggcggcgg
      481 ccggcgtgct gactcaggtg atggaccagg tgaagatggg cggggtgcag gatgtttcca
      541 tcgccgcgct tccgcagggt ggctgacgca tgttcctgat ccaatcgccc tttgcgcgcc
      601 cgttgctcta cgccggcatt tttgcgctga gcctgttctg gctgctgcat gcgctgatca
      661 accgtagcca cagcgtcgac aagagcgagg cgctgcagac ggtggatttc gtgcgtttgc
      721 gcaaggacac cgacatcgaa tcgcgcatcc gccagaagcc gcccaagccg caaccgccaa
      781 aggaaccgcc gccgccgaag atgcagacgg tggcgccgca ggagcggccg aaccagcagc
      841 ggctgccgtt caacattccc aacctgaaca ccgggctcaa tttcggtggc ggcccgttca
      901 tcggcaatct cgccgccgct gccgaagcag gcggctattc cgaactgatc cccctggtgg
      961 cattgcagcc gcagtatccg cgccaggcgg cacgcgacgg catcgagggc tgggtcgatg
     1021 tggagatcac catcgggcct gatggttccg tgaaatccgc gcgggccaag gacgccaagc
     1081 cgcgcggcgt gttcgaggcg gcagcgatca gcgctgcgct caagagccgc ttccgtccga
     1141 agatggtcga gggcaagggc gtggaggcca ctggtatccg ccgctacagc ttcaacctgg
     1201 gcaaagaatg atgcgcctgt ctatcaagct gcggttgctg ccgctgttgc tcgcgctggt
     1261 ctgtgccctg agcgcagccg tcatgccggc gccggcgatg gcggacgacc agccggcgcc
     1321 ggccaagaaa gagcgcaagg cgaccggttc gatgagcgag gggacctacc acgcgcttga
     1381 gaaggtctac aagctgatgg gcgacaacaa gaacgccgag gcgctggcgg ccttgcagac
     1441 gctgcaggat aaggtcagcg gcgtttacga gaagtgcatc gtgctgcaga ccatcggcta
     1501 cgtgtacgcc aaccagaaca actacaagaa ggcgatcgaa gtgttcgagc aggcgctcgc
     1561 gctcgacgcg ttgccgcagc agccgtacga gcagttgctc tacaacatcg cgcagatgga
     1621 tgtggcgacc gagcagtacg acaagggcat cgccatgatc gagcgctatt tcgccgaggc
     1681 cacctttgcc aagccgccgc cggattcgca catccttgcg gccagtgcct acgccgagaa
     1741 aaagcgtttt gccgatgcgc tcaagcaggt tgacatcgcg cttgccgaag ccaaggcacc
     1801 gaaggagccc tggctgcagc tcaagctggc cctgcactac gagctgaagc agtacccgca
     1861 gtgcgccgag gtgctgctgc agctgatcgc catcgcgccg accaagaagg attactggaa
     1921 gcagctgtcc agcatgttct tcgagatcaa ggaagacaag gaatcgctcg ccgtgctggc
     1981 gctggctgat cgccagggct atctcacgga agaccacgag taccgcaacc tggccaacgt
     2041 gtacatgctg ctggaaattc cgtacaaggc cgggcaggtg ctgcagcggg gctttgaccg
     2101 caagatcctc aaggaagacg agaagtcgct gaccatgctg gccgacgcat ggcagctggc
     2161 gcgcgaaggc gacaaggccg ccgaagcgct gcgccgtgcg gccgatgcta ccggcaaggg
     2221 cgacctgtac ctgcgcctgg ctggtgtcta cgccgaagat gagaaatgga agaaatcgct
     2281 ggaggcggtc aacctcgcgc tgagcaaggg cgtgaccaag accggtccgg cgctgctgct
     2341 tgcaggccag gtggcctatc acgccggcga caaggcggcg gcccggcgtg atctgaacaa
     2401 ggcgatggaa cacgaagatg tacgcaagcc ggcgcacgac tggctgcagt acatggagca
     2461 gatggaaggc gagcccgctg tggtggcgaa tgcttcgtcc ggtgcggacg atcagcagca
     2521 gtccgctggc gacaacaacg ccagtgcccc cgcacccagc gcgcctgcgg cacctgcagc
     2581 gccggctgcg cccgccaaga cggccgcacc gccttcgccg cccaagccgg caccgccgcc
     2641 cgccaggaag aagtgatcgg gacgccggct ccatg
//
LOCUS       DASZZC010000166         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1039223,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000166 DASZZC010000000
VERSION     DASZZC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039223"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1545)
                     /locus_tag="VHE37_03720"
     CDS             complement(<1..>1545)
                     /locus_tag="VHE37_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34663.1"
                     /translation="FSFRLAVDQGNPAIVYAATSKGLFRSADAGASYVNVALPVSSDC
                     AGVEALGPCQFANFVTDVVVKQPGGSTSVACDPKGCPVLAAVGFRSGMLPYPDTATKQ
                     SPGNGVYRSDTGEAGTFAMVGNYQITGVEPVGFTLKSRIGRVALGNAIGDAQNHDYVY
                     AMVQDAGYLNGDIALLDFDADIKDPTLPTGPVCSALALADPTLVDTCTQLASKVPSPT
                     TFNGLFVSTDFGDTWLRMADDKEITYNETTSSSIAPVIAIGEGPGIQAWYNLWVRPDP
                     TQQLAGAPTRVLFGLEEIWKNRLNVPQDGLAQAGPDDYHVIGTYFAGQTCLFLIGNAG
                     LPSLPVCPTYPGLVHGTTTHPDQHDGIFIPDTKNGGVWLHVGSDGGSYKQYSSDPTTD
                     DFANNKWSNGTNNDRYTLMVYGIAVAKDGTVYYGLQDNASGKIEPDTHRSVRIYIGDG
                     MWTAVDPDNSMNAYYSTPGLAVVATTDGGATDTDVYPPNAVRGTPHFLSPFVLDPTDA
                     NHIVMA"
BASE COUNT          239 a          475 c          555 g          276 t
ORIGIN      
        1 ccgccatgac gatgtggttg gcgtccgtgg gatcgagcac gaacggcgac aggaaatgcg
       61 gcgtgccgcg caccgcgttc ggcggatata catcggtgtc ggtggcgccg ccgtcggtgg
      121 tggcgaccac ggccaggccc ggcgtcgagt aataggcgtt catcgagttg tcaggatcga
      181 ccgcggtcca catgccgtcg ccgatgtaga tgcgcacgct gcggtgcgta tccggctcga
      241 tcttgccgct ggcgttgtcc tgcagcccgt aatacacggt gccgtccttg gccacggcga
      301 tgccgtacac catcagcgtg tagcggtcgt tgttggtgcc gttgctccac ttgttgttgg
      361 cgaagtcgtc ggtggtcgga tcggatgagt attgcttgta cgagccgccg tcgctgccca
      421 cgtgcagcca cacgccgcca ttcttcgtat cgggaatgaa gatgccgtcg tgctggtcgg
      481 ggtgcgtggt cgtgccatgc accaggcccg ggtaggtcgg gcacaccggc agcgacggca
      541 ggccggcgtt gccgatcagg aacaggcagg tctgcccggc gaagtaggtg ccgatgacgt
      601 gatagtcgtc ggggccggcc tgtgccagcc cgtcctgcgg cacgttgagc cggttcttcc
      661 agatttcctc caggccgaac agcacgcgcg tgggcgcgcc ggccagctgc tgcgtcgggt
      721 cgggccgcac ccacaggttg taccaggcct ggatgccggg cccttcgccg atggcgatga
      781 ccggcgcgat cgaagagctg gtggtctcgt tgtaggtgat ctccttgtcg tcggccatac
      841 gcagccaggt gtcgccgaaa tcggtggaga cgaacaggcc attgaaggtg gtcgggctcg
      901 gcaccttgct ggccagctgg gtgcaggtat caaccagcgt gggatcggcc agcgccagcg
      961 cgctgcacac cgggccggtc ggcagcgtcg ggtccttgat gtcggcatcg aagtccagca
     1021 gcgcgatgtc gccgttgaga tagccggcgt cctgcaccat ggcgtagacg tagtcgtggt
     1081 tctgcgcatc gccgatggcg ttgcccagcg ccacgcggcc gatgcgtgat ttcagcgtga
     1141 agccgaccgg ctcaacgccg gtgatctggt agttgccgac catggcaaaa gtgccggcct
     1201 cgccggtgtc ggagcggtac acgccattgc cgggcgactg cttggtggcg gtgtcaggat
     1261 acgggagcat gccggagcgg aagcccaccg ccgccagcac cgggcagccc ttcgggtcgc
     1321 aggccacgct ggtgctgccg ccgggctgct tgaccaccac gtcggtgacg aagttggcga
     1381 actggcacgg gccgagcgct tcgacgccgg cgcaatcgct cgacaccggc agcgcgacgt
     1441 tcacgtagct ggcgcccgca tcagccgagc ggaacaggcc cttgctggtc gcagcgtaaa
     1501 cgatggccgg attgccctgg tcgacggcga ggcggaaaga gaatg
//
LOCUS       DASZZC010000167         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_914199,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000167 DASZZC010000000
VERSION     DASZZC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914199"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1255
                     /locus_tag="VHE37_03725"
     CDS             152..1255
                     /locus_tag="VHE37_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT34664.1"
                     /translation="MSAVLATEKLVSVDSHVHFTDEWVKERLPKKLHAVWDEANRKAE
                     KYGAEVLRGGQPQLHLEDFVDPEAAKDPGHFNPEGKLKAMDLDGVYAEVIFPELAGAK
                     IVSPDLMGSDWKDVFVGYNNAMGDFAAHDPVRLMTAYQLPLYDIDFAVKEVQRLARDK
                     KARCVQLTPFPSDLGLPDFYHERYAPLWSAIQDANLTILNHLDLRASLWDVFRRDPTP
                     QKGIFTALPWAPLAETICMWILTGTLEKYPKLKVIFVEPGLGWLPWFFSALLDPRMHA
                     HYEFPGVKKPPSETFRAQCGATFMYEPLGLKLCYDYFGPDCLYWSTDFPHPATCWPNS
                     RKQVVSQFAEAGIPEADRKKITSLNALRTFGLG"
     gene            1376..3886
                     /locus_tag="VHE37_03730"
     CDS             1376..3886
                     /locus_tag="VHE37_03730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVT34665.1"
                     /translation="MAGVLNGVKVLDLSWGIAGPMTAMLMADHGADVTKIEPPGGDPF
                     RGQLGYKVWQRGKKSAVLDLKNAADKETFLALAKAADVLVESYAPGTAEKLGIDYKTL
                     SALNPRLVYCSITGYGRGNEHSNRPAYDALVAARTGLHFEQRGWPEGALNHLAGRPDP
                     FPDVEIPYDWLQGAPRPGPLFPASYWPSLGAFFNASVGISAALYAREHTGRGQLVETS
                     LMQGAMAGAAGVWMRAENIDADGFDSWILGCRAPKGHFQCKDGNWIHNWVPNPRFILT
                     ASEGDTLNATPNLTVQNDPDRFGTGPEELLVMAHYQPLLADAVKKFTAQEWTDAAETA
                     EMTMQPVRSVEASLADPLLLKDGCVRETVDPELGKIRTVGVTYKMSACPSTPGTHAPK
                     VGEHTAQVKAEAKSAKPAAGKASGKKIRAPLEGITVLDLGLAIAGPFGTQLLSDLGAN
                     VIKINGLFDLFWHRVHIAYMANRGKKSITLNLKDPRAMKIFIDLVKTADVVHHNMRYD
                     AAERLKIDYESLKQINPKLIYCHTRGFETGKRAGLPGNDQTGACLSGIQYEDGGMARG
                     GKPLWSLTSFGDTGNGFLSAVAVIQALMHRERTGEGQMVDTSIINAALLNTSYAVATP
                     EGKGFERPHIDGMQLGFSAGHRLYETKQGWLCLVLVNQSHWDELFTVLRAPGLVTDPK
                     FATPEARKQNDAALSQFIAERMHAHTAEEWFKELDAAGVPVEIVSEDFSRKLHDTPEF
                     QKRRWVVSYPHPVVGKLDQIGLLYDLSDTPGVIQGRPLLVGEHTKEILAGMGYTEEQV
                     KTMEEQMAIGFVGMPRMPGRKPGAPVPLTPSAAQSEKK"
BASE COUNT          770 a         1326 c         1266 g          631 t
ORIGIN      
        1 ttaataacac gcgcggaggc ggtgcggtgg ggcttttttc gcgcccgcgc cgcgctactc
       61 acccatttgc agcatgcttg cgcaacgcgc cgcggcgtaa ggtctgcgcg tcaaaatttc
      121 tccgcgcgcg gagaaccgga aggagaacgc catgagcgcc gtgctcgcca ccgagaaact
      181 ggtttccgtc gattcgcacg tgcacttcac cgacgagtgg gtgaaggagc gcctgccgaa
      241 aaagctgcac gccgtgtggg acgaggccaa tcgcaaggcc gagaaatacg gcgccgaggt
      301 gctgcgcggc ggccagccgc agctgcacct ggaagacttc gtcgacccgg aagcggcgaa
      361 agacccgggc cacttcaatc ccgagggcaa gctcaaggcg atggacctcg acggcgtcta
      421 tgccgaggtg atcttccccg agctggccgg cgccaagatc gtcagcccgg acctgatggg
      481 cagcgactgg aaggacgtgt tcgtcggcta caacaacgcc atgggcgatt tcgccgcgca
      541 tgacccggtg cgcctgatga cggcttatca acttcctctg tacgacatcg atttcgcggt
      601 caaggaagtg cagcgtctgg cgcgcgacaa aaaggcgcgc tgcgtgcagc tgacgccgtt
      661 cccgagcgac ctgggcctgc cggatttcta ccacgagcgc tacgcgccgc tgtggtcggc
      721 gatccaggac gccaacctca ccatcctcaa ccacctcgat ctgcgcgcct cgctgtggga
      781 cgtattccgc cgcgacccga cgccgcagaa gggcatcttc accgcgctgc cgtgggcgcc
      841 gttggccgag accatctgca tgtggatcct caccggcacg ctggagaaat acccgaagct
      901 gaaggtgatc ttcgtcgaac cgggcctggg ctggctgccc tggttcttct ccgcgctgct
      961 cgatccgcgc atgcacgcgc actacgaatt ccccggcgtg aaaaagccgc cgagcgaaac
     1021 cttccgcgcg cagtgcggcg ccaccttcat gtacgagccg ctgggcctga agctgtgcta
     1081 cgactacttc ggcccggatt gcctgtactg gtccaccgat tttccgcatc ccgccacctg
     1141 ctggccgaat tcgcgcaagc aggtggtgag ccagtttgcc gaggccggca tcccggaagc
     1201 ggatcgcaag aagatcacat cgctcaatgc gctgcgtacc ttcgggttgg gttgaaacac
     1261 aatgtcgtca ttcccgcgca ggcggcaatc cagtttttcg cgttgatgca caccgaccga
     1321 aaactggatc cccgccttcc cggggatgac gggaatttca aataacggtg aatctatggc
     1381 tggtgtactc aacggcgtaa aagttctcga cctcagctgg ggcatcgccg gcccgatgac
     1441 ggccatgctg atggccgatc acggcgcgga tgtcaccaag atcgagccgc ccgggggcga
     1501 tccgttccgc ggccagctcg gctacaaggt ctggcagcgc ggcaagaaga gcgcggtgct
     1561 cgatctcaag aacgccgccg acaaggaaac ctttctcgcg ctggccaagg ccgccgacgt
     1621 gctcgtcgaa agctatgcgc cgggcacggc agaaaaactc ggtatcgatt acaagacgct
     1681 gtccgcgctc aatccacgac tggtgtactg ctccatcacc ggttacggcc gtggcaacga
     1741 gcacagcaac cgcccggcgt acgacgcgct ggtcgccgcg cgcaccggcc tgcacttcga
     1801 gcagcgcggc tggccggaag gcgcgctcaa ccatctcgcc gggcggcccg atccgtttcc
     1861 cgatgtcgag attccctacg actggctgca gggcgcgccg cgtcccggcc cgctgttccc
     1921 ggcgtcgtac tggcccagcc tgggcgcgtt cttcaacgcc tcggtcggca tcagcgccgc
     1981 gctgtatgcg cgcgagcaca ccggtcgcgg ccagctggtg gaaacatcat tgatgcaggg
     2041 cgccatggcc ggcgccgccg gcgtgtggat gcgcgcggag aacatcgatg ccgacggctt
     2101 cgactcgtgg atcctgggct gccgcgcgcc caaggggcat ttccagtgca aggacggcaa
     2161 ctggatccac aactgggtgc cgaacccgcg cttcattctt accgcgtcgg aaggcgacac
     2221 gctcaacgcg acgcccaacc tcacggtgca gaacgatccg gaccgcttcg gcaccggtcc
     2281 tgaagaactg ctggtgatgg cgcactacca gccgctgctg gccgacgccg tgaagaagtt
     2341 caccgcgcag gaatggaccg acgcggcgga aacggctgaa atgaccatgc agccggtgcg
     2401 ctcggtggaa gcctcgctgg ccgatccgct gttgctgaaa gacggctgcg tgcgcgagac
     2461 ggtcgatccc gagctgggaa aaatccgcac cgtgggcgtc acctacaaga tgagcgcgtg
     2521 cccgagcacg cccggcacgc acgcgccgaa agtcggcgag cacaccgcgc aggtcaaggc
     2581 cgaggcgaag agcgcgaagc cggcggcggg caaggcatcc ggcaagaaga tcagggcgcc
     2641 gctggaaggc atcacggtgc tcgatctcgg tcttgccatc gccggcccgt tcggcaccca
     2701 gctgctgtcg gacctcggcg ccaacgtcat caagatcaac gggctgttcg acctgttctg
     2761 gcaccgcgtg cacatcgcct acatggccaa ccgcggcaag aagtcgatca cgttgaacct
     2821 gaaagacccg cgcgcgatga agatcttcat cgacctggtg aagaccgccg acgtggtcca
     2881 ccacaacatg cgctacgacg cggccgaacg cctgaagatc gattacgaaa gcctcaagca
     2941 gatcaatccg aaactgatct actgccacac gcgcggcttc gaaaccggca agcgtgccgg
     3001 cctgcccggc aacgaccaga ccggcgcctg cctgtccggt atccagtacg aggacggcgg
     3061 catggcgcgc ggcggcaagc cgctgtggag cctgacttcg ttcggcgata ccggcaacgg
     3121 cttcctctcc gccgtcgccg tgatccaggc gctgatgcac cgcgagcgca ccggtgaggg
     3181 ccagatggtc gacacctcca tcatcaacgc cgcgctgctc aacaccagct atgccgttgc
     3241 cacgccggaa ggcaagggct tcgagcgtcc gcacatcgac ggcatgcagc tgggtttctc
     3301 cgcgggccat cgcctgtatg aaacgaaaca gggctggctg tgcctggtgc tggtcaacca
     3361 gtcgcactgg gatgaactgt tcaccgtgct gcgcgcgccg ggcctggtca ccgacccgaa
     3421 gttcgcgacg cccgaagcgc gcaagcagaa cgatgccgca ctctcgcagt tcatcgccga
     3481 gcgcatgcac gcgcacaccg ccgaggaatg gttcaaggaa ctcgacgccg ccggcgtgcc
     3541 ggtggaaatc gtcagcgaag acttctcgcg caagctgcac gacacgccgg aattccagaa
     3601 acgccgctgg gtggtgagct acccgcaccc ggtagtcggc aagcttgacc agatcggttt
     3661 gctctacgac ctgtccgata cgccgggcgt gatccagggc cggccgctgc tggtgggcga
     3721 gcacaccaag gagatcctcg ccggcatggg ttacaccgag gagcaggtga agaccatgga
     3781 agagcagatg gcgatcggct tcgtcggcat gccgcgcatg ccgggtcgca agccgggcgc
     3841 gccggtgccg ctgacgcctt cggcggcgca gagcgagaag aaataacgcg gtggacgagg
     3901 gcagcgcgca tcggcaggat tcgcgcatgc ccaagaccgc gccgtacttc acgcccgcca
     3961 gcttcaagtt cctgcgcgac ctcaagcgca aca
//
LOCUS       DASZZC010000168         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_269803,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000168 DASZZC010000000
VERSION     DASZZC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269803"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VHE37_03735"
     CDS             complement(<1..887)
                     /locus_tag="VHE37_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT34666.1"
                     /translation="MQLNRIDMNLLVVLETIYSEGGITRAADKLHLTQPALSHSLARL
                     RELFKDPLFVREGRAMVPTPLARKLIEPLRRSLRAMETALNEVQRFDPATTQRRFAIG
                     LRDVLEATVLPPLMKRISSMAPQVDVAALRLERRELEAELASGTLDAALDVLLPHGDR
                     VKHQRLALDKLVVVARRNHPVVRKTLDLKTYLDLGHVAVSSRRAGPGLEDTELARRGL
                     QRRIRLRCQHYFAACQVVARSDLLLTMPERYAGVANQTSGNRILPLPFKAPSLDAYLY
                     WHVNVDQEPASCWLREQLVA"
     gene            981..1751
                     /locus_tag="VHE37_03740"
     CDS             981..1751
                     /locus_tag="VHE37_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT34667.1"
                     /translation="MAVDPMFDLTGKVALVTGASRGIGEAIARTLGAHGALVIVSSRK
                     AADCERVAADIRAAGGKAEVRACHVGDFEQTQNIFREIENAHGRLDILVNNAAANPYF
                     GPICDTDLTAYNKTVDVNIRGYFFMCAAGAKLMKKNGGGSIVNVASIGGKVPGDFMGI
                     YSVTKAAVISMTKAFAKEVASMGVRVNALLPGLTETKFAGALVTNEAIRKQATARIPM
                     RRVAQPSEMAGAVLYLVSPAASYTTGSELVVDGGYLQG"
     gene            1764..>1942
                     /locus_tag="VHE37_03745"
     CDS             1764..>1942
                     /locus_tag="VHE37_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT34668.1"
                     /translation="MKQLQGKVAVITGAGSGFGRELAILCAGEGMKLVLADIQENAIA
                     ETVKLLPAGTQTLGV"
BASE COUNT          322 a          632 c          683 g          305 t
ORIGIN      
        1 gccaccagct gctcgcgcag ccagcagctg gcgggttcct ggtcgacgtt cacgtgccag
       61 tacaggtagg cgtcgagcga cggcgccttg aacggcagcg gcaggatgcg gttgccggag
      121 gtctggttgg ccacgccggc atagcgctcg ggcatggtca gcagcaggtc ggaacgcgcc
      181 accacctggc aggcggcgaa gtagtgctgg cagcgcaggc ggatgcgccg ctgcagcccg
      241 cggcgcgcaa gctcggtgtc ttccaggccc ggcccggcgc ggcgcgaact caccgcgaca
      301 tggccgagat cgagataggt tttcaggtcg agcgtcttgc gcaccacggg gtgattgcgc
      361 cgcgccacca ccaccagctt gtccagcgcc aggcgctggt gcttcacgcg gtcgccgtgc
      421 ggcagcagca cgtcgagcgc ggcgtcgagg gtgccggagg ccagttccgc ctccagttcg
      481 cgccgctcca gccgcagcgc ggcgacgtcc acctgcggcg ccatgctgct gatgcgcttc
      541 atcagcggcg gcagcacggt ggcttccagc acgtcgcgca ggccgatggc gaagcgccgc
      601 tgcgtggtcg ccgggtcgaa gcgctgcacc tcgtttagcg cggtttccat ggcgcgcagc
      661 gagcggcgca gcggctcgat cagcttgcgc gccagcggcg tcggcaccat ggcgcggcct
      721 tcgcgcacga acagcgggtc cttgaacagt tcgcgcaggc gcgccagcga gtgcgacagc
      781 gccggctgcg tcaggtgcag cttgtcggcg gcgcgggtga tgccgccctc gctgtagatg
      841 gtttccagca ccaccagcag gttcatgtcg atgcggttga gttgcatgcg gcggacttat
      901 accaggcgat ccggattatt catttgtccg attctaattc cgcgcctagg ctggcgtcct
      961 catttttcag gagcgcgcgc atggccgtgg acccgatgtt cgacctcacc ggaaaagtcg
     1021 ccctggtcac cggcgccagc cgcggtatcg gcgaggccat cgctaggacg ctgggcgcgc
     1081 acggcgcgct ggtcatcgtc tccagccgca aggcggcgga ctgcgagcgc gtggccgcgg
     1141 acatccgcgc cgccggcggc aaggccgagg tgcgcgcctg ccatgtcggt gacttcgagc
     1201 agacgcagaa catcttcagg gagatcgaaa acgcgcacgg gcggctcgac atcctggtca
     1261 acaacgccgc cgccaacccg tatttcggcc cgatctgcga taccgacctc accgcctaca
     1321 acaagacggt ggacgtgaac atccgcggct atttcttcat gtgcgcggcc ggcgccaagc
     1381 tgatgaagaa gaacggcggc gggtccatcg tcaacgtcgc ctccatcggc ggcaaggttc
     1441 cgggtgactt catgggcatc tactcggtga ccaaggccgc ggtgatctcc atgaccaagg
     1501 ccttcgccaa ggaagtggcg tcgatgggcg tgcgcgtcaa cgcgctgctg ccgggcctga
     1561 ccgaaacgaa attcgccggt gcgctggtga ccaacgaggc catccgcaag caggccaccg
     1621 cgcgcatccc gatgcggcgc gtggcccagc cgagcgaaat ggccggcgca gtgctgtacc
     1681 tggtgtcgcc ggcggcgagc tacaccaccg gcagcgagtt ggtggtcgat ggcggttatc
     1741 tccagggttg agcggagcgc accatgaaac agctgcaggg aaaagtagcg gtgatcaccg
     1801 gtgccggcag cggcttcggc cgcgagctgg ccatcctgtg tgcgggcgaa gggatgaagc
     1861 tggtgctggc cgacatccag gaaaacgcca tcgccgaaac ggtgaagctg ctgccggcgg
     1921 gtacgcagac gctgggcgtg aa
//
LOCUS       DASZZC010000169         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_781916,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000169 DASZZC010000000
VERSION     DASZZC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781916"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VHE37_03750"
     CDS             complement(<1..1008)
                     /locus_tag="VHE37_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HVT34669.1"
                     /translation="MQRRDFLIAGAAAASVLPLRGFAAASAQPFDYAWLKGQARALAG
                     RAYVAPSRELPEALAALDYDGYQSIRFKPERSLWYGDDLAFRAQFFHRGYRLREAVRI
                     HEIVNGQARELAYDAAQYDFAKTGIKGSRLPHDLGYAGFRLQFRTNWAGEFMVFLGAS
                     YFRAIGGDNRQYGTSARGLAIDVGQDSGEEFPRFTEFWLERPAAASAKLVVYALLDSP
                     SVSGAYRFEIAPGNLTVMLCDVALYPRREIKHLGLAPLTSMFYTGENDRHGADWRPEI
                     HDSDGLALWTGGGEWIWRPLVNPPGTHLNSFLDQKPHGYGLLQRDRDFDHFLDDAVYY
                     EH"
     gene            complement(1064..>1570)
                     /locus_tag="VHE37_03755"
     CDS             complement(1064..>1570)
                     /locus_tag="VHE37_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HVT34670.1"
                     /translation="WELLGALAARGVRFMTLTHAAGISSTGDELLDRRWPLPEAYRIP
                     AATARAVATTRTQGGRIVAMGTTVVRALEHAAAGDGQVRAGEGLATQRIGPHTRLRVV
                     DAIVSGTHEAGTSHYELLQAFAALPVLARAARELDAHAYRTHEFGDSVLVERHAAEGS
                     DCGCARAA"
BASE COUNT          245 a          546 c          550 g          229 t
ORIGIN      
        1 atgctcgtag tacaccgcgt catcgagaaa gtgatcgaaa tcgcggtcgc gctgcagcag
       61 gccgtagccg tgcggcttct ggtcaaggaa ggaattcagg tgcgtgcccg gcgggttcac
      121 cagcggccgc cagatccact cgccgccgcc ggtccacagc gccagcccgt cggaatcgtg
      181 gatctccggc cgccagtcgg cgccatgacg atcgttctcg ccggtgtaga acatgctggt
      241 cagcggcgcc aggcccaggt gcttgatctc gcggcgcggg tacagcgcca cgtcgcacag
      301 catcacggtg agattgcccg gcgcgatctc gaagcggtac gcgccgctca cgctgggcga
      361 atcgagcagc gcgtagacca cgagttttgc cgaggccgcc gccggccgtt ccagccagaa
      421 ctcggtgaag cgcggaaatt cctcgccgct gtcctggccc acgtcgatgg ccaggccgcg
      481 cgcggacgtg ccgtactggc ggttgtcgcc gccgatggcg cggaagtagc tggcgccgag
      541 aaacaccatg aactcgccag cccagttggt gcggaactgc aggcggaagc cggcgtagcc
      601 caggtcgtgc ggcagcctgc tgcccttgat gccggtcttg gcgaagtcgt actgcgcggc
      661 gtcataggcc agctcgcgcg cctggccgtt gacgatctcg tggatgcgca ccgcctcgcg
      721 caggcgatag ccgcgatgga agaattgcgc acggaaagca agatcgtcgc cgtaccacag
      781 cgagcgctcc ggtttgaagc ggatcgactg gtagccgtcg taatccagcg cggccagcgc
      841 ttcgggcagc tcgcgcgacg gcgcaacgta ggcgcgcccg gcgagcgcgc gcgcctggcc
      901 cttcagccac gcgtaatcga aaggctgggc tgatgcggca gcgaagccgc gcagcggcag
      961 tacgctggcc gcggcggcgc cggcgatgag gaagtctcgt ctttgcatgc gcgcagtgta
     1021 agtcaggcag ccttgccact ggacgcccgc gctgccggat agttcaggcc gcccgcgcgc
     1081 aaccgcagtc gctgccctca gccgcgtggc gctccaccag caccgagtcg ccgaattcgt
     1141 gcgtgcggta tgcgtgggca tccagctcgc gcgcggcgcg cgccagcacc ggcagggcgg
     1201 cgaaggcctg cagcaattcg taatggctgg tgccggcttc gtgcgtgccg gaaacgatcg
     1261 cgtcgaccac gcgcagccgc gtgtgcgggc cgatgcgctg cgtggccagg ccttcgccgg
     1321 cacgcacctg gccgtcgccc gccgccgcat gctccagtgc gcgcaccacc gtcgtgccca
     1381 tggccacgat gcggccgccc tgcgtacgcg tcgtcgcaac ggcgcgcgcc gtcgccgcag
     1441 gaatgcggta cgcctccgga agcggccaac gccggtcgag cagttcgtcg ccggtggagg
     1501 agatgcccgc cgcgtgcgtc agcgtcatga agcgcacgcc gcgcgccgcg agcgcgccca
     1561 gcagttccca
//
LOCUS       DASZZC010000170         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_15583,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000170 DASZZC010000000
VERSION     DASZZC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15583"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1450)
                     /gene="nuoF"
                     /locus_tag="VHE37_03760"
     CDS             complement(113..1450)
                     /gene="nuoF"
                     /locus_tag="VHE37_03760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HVT34671.1"
                     /translation="MRPDGAPLNLADYEATGGYTAVRALRQLAPADVQQKVKDSNIRG
                     RGGAGFPAGIKWSLVPMGPDAGTKYLVCNADEMEPGTFKDRWLMESDPHALVEAMIVA
                     GYGIQATTGYIFLRGEYVVAAKHLNAALNEARAKGYLGKNIQGTGWTYDVHVHTGAGR
                     YICGEETALINSLEGKRANPRAKPPFPQISGLWGRPTVVNNVETLCNVPHIVNNGGDW
                     FKGLNQGKSKDAGTKLYGVSGRAKHPGCWEMPMGSTARELLEGQAGGMKDGLKLKAWL
                     PGGASTDFLTEQHLDLAMDFDTIAKAGSRMGTGLITVIDDSQSMITVVRNLEEFFSRE
                     SCGWCTPCRDGLPWTVRILLALERGQGKPEDLDTLARMTRLLGPGKTFCAHAPGAMEP
                     LQSALKYFRGEFEQGLAECRVHQAGDVCEVPESVHAQHADRTRISKNDHAAQA"
BASE COUNT          234 a          509 c          486 g          294 t
ORIGIN      
        1 cagcagaaat acggaatgtc gagcccgagg gacaggcagg cctggagcag gttgtccgcc
       61 ccgttcactt cgtactgctt gccgtcgacg tggatgatcg ccatgtgtct atctatgcct
      121 gcgcagcgtg atcgttcttg ctgatgcgcg tgcggtcggc atgctgggca tgcaccgact
      181 ccggcacttc gcacacatca ccagcctggt gcacgcggca ttcagccagt ccctgctcga
      241 actccccgcg gaaatacttg agcgccgatt gcagcggctc catcgcgccc ggcgcatgcg
      301 cgcagaacgt cttgccgggg ccaagcaggc gcgtcatgcg cgccagcgtg tcgaggtcct
      361 cgggtttgcc ctggccgcgc tcgagcgcca gcaggatgcg cacggtccac ggcagtccgt
      421 cgcggcacgg cgtgcaccag ccgcaggatt cgcgcgagaa gaactcctcc agattgcgca
      481 ccacggtgat catgctctgg ctgtcgtcaa tcaccgtgat caggccggtg cccatgcgcg
      541 agcccgcctt ggcgatggtg tcgaaatcca ttgccaggtc gagatgctgc tcggtgagaa
      601 aatcggtgga ggctccgccg ggcagccagg ccttgagctt gaggccatcc ttcatgccgc
      661 ctgcctggcc ttcgagcagt tcgcgcgcgg tcgagcccat cggcatttcc cagcagccgg
      721 gatgcttcgc tcggccggag acgccataaa gcttggtccc ggcatccttg gacttgccct
      781 ggttcaggcc cttgaaccag tcgccgccgt tgttgacgat gtgcggcacg ttgcacagcg
      841 tttcgacgtt gttgaccacc gtcgggcgcc cccacaggcc gctgatctgc gggaacggcg
      901 gcttggcgcg cgggttggca cgcttgcctt ccagcgaatt gatcagcgcg gtttcctcgc
      961 cgcagatgta gcggccggcg ccggtgtgca cgtgcacgtc ataggtccag ccggtgccct
     1021 ggatgttctt gcccaggtag cccttggcgc gcgcctcgtt caacgccgcg ttgaggtgct
     1081 tcgccgcaac cacatattcg ccgcgcagga aaatgtagcc ggtagtggcc tggatgccgt
     1141 agcccgccac gatcatggcc tcgaccagcg catgagggtc ggactccatc agccagcggt
     1201 ccttgaaggt gccgggctcc atctcgtccg cgttgcatac caggtacttg gtgccggcat
     1261 ctggccccat cggcaccagg ctccacttga tgccggccgg gaaccccgca ccgccacgac
     1321 cgcggatgtt ggaatccttg accttctgct gcacgtcggc gggcgccagt tgcctgagcg
     1381 cgcgcactgc ggtgtagccg ccggtagctt cgtaatccgc caggttcagc ggcgcgccat
     1441 caggacgcat gcgccccgtg agcgggtgag tctgcaactt cttttcagcg gcggcgttca
     1501 cttgtacggc tccagcaact ggc
//
LOCUS       DASZZC010000171         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_722916,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000171 DASZZC010000000
VERSION     DASZZC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722916"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VHE37_03765"
     CDS             <1..804
                     /locus_tag="VHE37_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HVT34672.1"
                     /translation="GSAVPVARTHVLCEDESIIGTMFYVMDCVDGRVFWDGSLPELTK
                     AERAAIYADLNRVIAALHQVDYARAGLADYGKPGNYFERQIGRWTKQYRASETETIEA
                     MDKLMFWLPKNIPPGDETTLVHGDFRLDNVIIHPSEPRIIAVLDWELSTLGHPLADFA
                     YHCMTWHLPAGPFRGIAGHDPVKLGIPTEAEYVAMYCRATGRAPIPQDHWDFYMAFNL
                     FRGAAILQGIMGRVKDGTAASAHALEAGRAARGVGELGWQQVEKILSRT"
     gene            826..2076
                     /locus_tag="VHE37_03770"
     CDS             826..2076
                     /locus_tag="VHE37_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT34673.1"
                     /translation="MDFEYSQKTRDLLKRLNAFFDEHIYPNEDQFLKEVNTGDRWQPT
                     QIMETLKAKAKAAGLWNLFLPESHRGAGLTNLEYAPLCEVMGRVIWSPEVFNCSAPDT
                     GNMEVLERYGSDEQKKQWLEPLLAGEIRSCFAMTEPLVASSDATNIECRIEKDGADYV
                     INGRKWWSSGACDPRCKVFILMGKTDPNNASRHSQQSMILVPREAKGVNILRHLPVFG
                     YDDAPHGHAEILFENVRVPQSNILLGEGRGFEIAQGRLGPGRIHHCMRLIGLAERVLE
                     KMCKRSLERVAFGKPVAAQTVTLERIAEARILIDQARLLTLKAAYMMDTVGNKVAAKE
                     IAMIKVAAPNMACQVIDWAIQAHGGGGVTDQFGLSYAYAQARTLRLADGPDEVHRNQI
                     GKLELARYVSPEVAKMLSASRRGK"
BASE COUNT          398 a          731 c          716 g          325 t
ORIGIN      
        1 ggcagcgccg tgccggtggc gcgtacgcat gtgctgtgcg aagacgagtc catcatcggc
       61 acgatgttct acgtgatgga ttgcgtggac ggccgcgtgt tctgggacgg ttcgctgccg
      121 gagttgacca aggccgagcg cgcggcaatc tacgccgacc tcaaccgcgt gatcgccgcg
      181 ctgcatcagg tcgattacgc ccgcgccggt ctcgccgact acggcaagcc gggcaactac
      241 ttcgagcgcc agatcggccg ctggaccaag cagtaccgcg cgtccgagac cgaaaccatc
      301 gaggcgatgg acaagctgat gttctggctg ccgaagaaca tcccgccggg cgacgagacc
      361 acgctggtgc acggcgactt ccgcctggac aacgtcatca tccatccgag cgagccgcgc
      421 atcatcgcgg tgctcgactg ggaactgtcc acgctcggcc atccgctggc cgacttcgcc
      481 tatcactgca tgacctggca cctgccggcc gggccgttcc gcggcatcgc cgggcatgac
      541 ccggtgaagc tcggcattcc gaccgaggcc gagtacgtgg cgatgtactg ccgcgccacc
      601 ggccgcgcgc cgatcccgca ggatcactgg gacttctaca tggccttcaa cctgttccgc
      661 ggcgcggcga tcctgcaggg gatcatgggc cgcgtgaagg acggcaccgc cgccagcgcg
      721 catgcgctgg aagccgggcg cgccgcgcgc ggcgtgggcg agctgggctg gcagcaggtc
      781 gaaaaaattc tctcccgcac ctaacgcatt caacggagca cgacgatgga cttcgagtac
      841 tcgcaaaaaa ccagggacct gctcaagcgc ctcaacgcgt ttttcgacga gcacatctat
      901 cccaacgagg atcagttcct gaaggaggtc aacaccggcg accgctggca gcccacccag
      961 atcatggaaa cgctcaaggc caaggccaag gccgccggcc tgtggaacct gttcctgccc
     1021 gagtcgcacc gcggcgccgg cctcaccaac ctggaatacg cgccgctgtg cgaagtgatg
     1081 ggccgcgtga tctggtcgcc ggaagtgttc aactgctcgg cgccggacac cggcaacatg
     1141 gaggtgctgg agcgctacgg cagcgacgag cagaagaagc agtggctcga accgctgctc
     1201 gccggagaga tccgcagctg cttcgccatg accgagccgc tggttgcctc gagcgatgca
     1261 accaacatcg agtgccgcat cgagaaggat ggcgccgact acgtcatcaa cgggcgcaag
     1321 tggtggagct ccggcgcctg cgatccgcgc tgcaaggtct tcatcctcat gggcaagacc
     1381 gaccccaaca acgccagccg ccacagccag cagtcgatga tcctggtgcc gcgcgaggcc
     1441 aagggcgtca acatcctgcg ccacctgccg gtgttcggct acgacgacgc gccgcacggc
     1501 cacgccgaga tcctgttcga aaacgtgcgc gtgccgcagt cgaacatcct gctgggcgaa
     1561 ggccgcggct tcgagatcgc gcagggccgc ctcggccccg gccgcatcca ccactgcatg
     1621 cggctcattg gtctcgcgga gcgcgtgctg gagaagatgt gcaagcgctc gctcgagcgc
     1681 gtcgccttcg gcaagccggt cgccgcgcag acggtgacgc tggagcgcat cgccgaggcg
     1741 cgcatcctga tcgaccaggc gcgcctgctc acgctcaagg ccgcgtacat gatggacacg
     1801 gtgggcaaca aggtggcggc caaggagatc gccatgatca aggtggcggc gcccaacatg
     1861 gcctgccagg tgatcgactg ggccatccag gcgcatggcg gcggcggcgt caccgaccag
     1921 ttcggcctgt cgtatgcgta cgcgcaggcg cgcacgctgc gcctggccga tggtcccgac
     1981 gaagtgcatc gcaaccagat cggcaaactc gaactggcgc gctacgtgtc gccggaagtg
     2041 gcgaagatgc tgtcggcgag ccggcgcggg aagtgatgcc gcgcagcggc gtcatccccg
     2101 cgaaggcggg gatccagttt tgatctccgc cgctttcgac agcctgctca accgttggcg
     2161 tgcggcggcg
//
LOCUS       DASZZC010000172         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_977109,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000172 DASZZC010000000
VERSION     DASZZC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977109"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1596)
                     /locus_tag="VHE37_03775"
     CDS             complement(4..1596)
                     /locus_tag="VHE37_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVT34674.1"
                     /translation="MNDAVFGSAGMTPDQRATAQRRAWIGFCAMMFGNFMAILDIQIV
                     ASSLNEIQAGLSASADELTWVQTSYLIAEIVAIPLSGFMSRLLSTRIYFVMSALGFAL
                     SSLACAFAWNIGSMVAFRALQGFLGAGMIPTTMGSIFVMFPPDKRVLPTVLVGMVTTC
                     APALGPTIGGYLTNALTWHWLFLINLVPGLIIPGIVWAYVRLDQPDWKLLRNIDFPGL
                     LFMAMFLGGLEYSLDEGPRHDWLADEAVRNMALVASTGALAFFYRMFTAAHPIVDLRV
                     YANRNFSVGAVIAFVVGLMLYGIVFMVPTFFGQVRGYNSLQIGHVMMFTGVAMFFSAP
                     LVGKLSSKIDVRLLLAYGLCMVSLGTFLNSHLDAQADMWTFAIPQILRGHGFICCMIP
                     MTGLALGTLAPHEVKNGSGLFNVMRNLGGAIGLAVINTELAQRRSFHWAHLSTAIDPT
                     RAPVRAIINQGSTVLAPEMGGDSTTATVAMIGRLVARESAVMAFNDVFRAMSVVVAVT
                     LLLILLVKKPPPMLGAAAAEAH"
     gene            complement(1593..1742)
                     /locus_tag="VHE37_03780"
     CDS             complement(1593..1742)
                     /locus_tag="VHE37_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34675.1"
                     /translation="MNDALFGSAGMTPEERAAAQSRAWIGFCALTMYGNCMRFHAAAV
                     WERLP"
     gene            complement(1765..>2723)
                     /locus_tag="VHE37_03785"
     CDS             complement(1765..>2723)
                     /locus_tag="VHE37_03785"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVT34676.1"
                     /translation="IESTDNAYVRADITAITPRIAGEVTQVLVHDNQAVKKGDLLLAI
                     DAADYQARAASAAAQVAVREAALAANVQQKATQQSLLDEARATLAAAQADEARARKDW
                     ERADTLVKQEVATHQRLDTAVAAYQSAKAAVTRAEAGVKAAEQQTAMMDTDRARLSAE
                     LDAARAAQKLAEIDLAATELRAPVDGVIGDLAARVGERVNAGMRLLSVVPLAAVYVEA
                     NYKETQLTHMAIGQQAEIEIDAFPGRKLRGHIDSLSPASGAEFSLLPPDNATGNFNKI
                     VQRVPVKIVFDEAGELAGLLRPGMSVEATIDTRTAPATVAAR"
BASE COUNT          444 a          915 c          954 g          410 t
ORIGIN      
        1 agttcagtgc gcctcggccg ccgcggcgcc gagcatcggc ggcggcttct ttaccagcag
       61 gatcagcagc agcgtcaccg ccaccaccac cgacatggcg cggaagacgt cgttgaacgc
      121 catcaccgcc gactcgcgcg ccaccagccg gccgatcatc gccacggtag cggtggtgct
      181 gtcgccgccc atttcgggtg ccagcacggt gctgccctgg ttgatgatcg cgcgcaccgg
      241 cgcgcgcgtg gggtcgatgg cggtagacag gtgcgcccag tggaacgagc ggcgctgcgc
      301 cagctcggtg ttgatcaccg ccagcccgat ggcgccgccc aggttgcgca tcacgttgaa
      361 caggccggaa ccgttcttca cctcgtgcgg agccagcgtg ccgagcgcca gcccggtcat
      421 cgggatcatg cagcagatga agccgtgccc gcgcaggatc tgcgggatgg cgaaagtcca
      481 catgtcggcc tgcgcatcca ggtgcgaatt gaggaaggtg cccagcgaga ccatgcacag
      541 gccgtaggcc agcagcaggc gcacgtcgat cttcgacgac agcttgccca ccagcggcgc
      601 cgagaaaaac atggccacgc cggtgaacat catgacgtgg ccgatctgca ggctgttgta
      661 gccgcgcacc tggccgaaga aggtgggcac catgaagacg atgccgtaca gcatcaggcc
      721 gacgacgaag gcgatgaccg cgcccaccga gaaattgcgg ttggcataga cgcgcaggtc
      781 gacgatcggg tgcgcggcgg tgaacatgcg gtagaaaaag gccagcgcgc cggtggacgc
      841 caccagcgcc atgttgcgca ccgcttcgtc ggccagccag tcgtggcgcg ggccttcgtc
      901 cagcgagtat tcaagcccgc cgaggaacat ggccatgaac agcaggccgg ggaagtcgat
      961 gttgcgcagc agcttccagt ccggctgatc cagccgcacg taggcccaga caatgccggg
     1021 gatgatcagg cccggcacca ggttgatcag gaacagccag tgccaggtga gcgcgttggt
     1081 gagatagccg ccgatggtcg ggcccagcgc cggcgcgcag gtggtgacca tgcccaccag
     1141 caccgtcggc agcacgcgct tgtccggcgg gaacatgacg aagatcgagc ccatcgtcgt
     1201 cgggatcatg ccggcgccga gaaagccctg cagcgcgcgg aacgcgacca tgctgccgat
     1261 attccaggcg aaggcgcagg ccagcgacga cagcgcgaag cccagcgccg acatgacgaa
     1321 gtagatgcgc gtggacagca ggcgcgacat gaagccggac agcgggatgg cgacgatctc
     1381 ggcgatgaga tagctggtct gcacccaggt cagctcgtcg gcgctggccg acagcccggc
     1441 ctggatctcg ttcagcgacg aggcgacgat ctggatgtcg aggatcgcca tgaaattgcc
     1501 gaacatcatg gcgcagaagc cgatccacgc gcggcgttga gcagtggccc gctggtcggg
     1561 ggtcatgccc gcggagccga atacggcgtc attcacggca aacgctccca aacggctgca
     1621 gcgtgaaacc tcatgcagtt gccgtacatc gtcagggcgc agaagccgat ccatgcgcga
     1681 ctttgcgccg cggcgcgttc ttccggcgtc atgccggcgg agccgaacag cgcgtcattc
     1741 atggcgggcg gcccagcgca tgcctcagcg cgccgccacc gtcgccggcg cggtgcgcgt
     1801 gtcgatggtg gcttccaccg acatgcccgg acgcagcagg ccggccagct cgccggcctc
     1861 gtcgaacacg atcttcaccg gcacgcgctg cacgattttg ttgaagttgc cggtggcgtt
     1921 gtcgggcggc agcagcgaga actcggcgcc ggacgcgggc gacagcgaat cgatgtggcc
     1981 gcgcagcttg cgcccgggga aggcgtcgat ctcgatctct gcctgctggc cgatggccat
     2041 gtgcgtcagc tgcgtctcct tgtagttggc ctcgacgtag accgcggcca gcggcaccac
     2101 cgacagcagg cgcatgccgg cgttgacgcg ctcgcccacg cgcgcggcca ggtcgccgat
     2161 gacgccgtcg accggcgcgc gcagctcggt ggcggccagg tcgatctcgg ccagcttctg
     2221 cgcggcgcgc gcggcgtcga gctcggcgct gagtcgcgcg cggtcggtgt ccatcatcgc
     2281 ggtctgctgc tcggcggcct tcacgccggc ttcggcgcgc gtcaccgcgg ccttcgccga
     2341 ctggtacgcg gccaccgcgg tgtcgagccg ctggtgcgtg gccacttcct gcttgaccag
     2401 cgtgtcggcg cgctcccagt ccttgcgcgc gcgcgcctcg tcggcctgcg cggcggccag
     2461 cgtggcgcgc gcctcgtcca gcaacgattg ctgcgtggcc ttctgctgca cgttggcggc
     2521 cagtgccgcc tcgcgcacgg ccacctgggc ggcggcgctg gcggcgcgcg cctggtagtc
     2581 ggcggcatcg atggccagca gcaggtcgcc cttcttcacc gcctggttgt cgtgcaccag
     2641 cacctgcgtc acctcgccgg cgatgcgcgg cgtgatggcg gtgatgtcgg cgcgcacgta
     2701 ggcgttgtcg gtgctctcga tgt
//
LOCUS       DASZZC010000173         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1235121,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000173 DASZZC010000000
VERSION     DASZZC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235121"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VHE37_03790"
     CDS             complement(<1..762)
                     /locus_tag="VHE37_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34677.1"
                     /translation="MAGFYFFYYATVGAFMPFWSPYLAARGFSAAQMGVAFALMGMMR
                     TFVPIAWGTLADRSGRRVRLIRIASVLSLVTFMGIPFVHGVFWVGALMLAYTVFWHAL
                     LPQFEAVALTHLGLSGGDYARVRLWGSVGFIVSVLGLGYALDITGIIALPWLVGGAWL
                     AMAASTFRVPDAPVMHHADAPQGSIREVLRRPEVLALLAVCLASQLSFAPYYNFFTLF
                     LERHGYTRGQAGFLWALGVIAEIGMFANMRKLIPQV"
     gene            complement(800..1897)
                     /gene="aroC"
                     /locus_tag="VHE37_03795"
     CDS             complement(800..1897)
                     /gene="aroC"
                     /locus_tag="VHE37_03795"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HVT34678.1"
                     /translation="MSGNTIGKLFCVTSFGESHGAALGCVVDGCPPGLELSETDIQPE
                     LDRRKPGTSKYVTQRKEEDKVEILSGVFEGRTTGTPIALLIRNMDQRSYDYEKIKQVF
                     RPNHADYTYMQKYGIRDHRGGGRSSARETAVRVAAGAIARKYLREKLGVRIRGCLEQM
                     GAITLERRDWSAVNANPFFCADPERVPELEALIDRMRSEGDSVGALIYVEASGVPPGL
                     GEPVYDRLDADLAYALMSINAVKGVEIGDGMKVAAMKGSEHRDEMSPQGFLSNHSGGI
                     LGGISSGQTIWARMAVKPTSSMSMPGQTIDQDGHSAQVRTTGRHDPCVGVRATPIAEA
                     MVALVLMDHFLRHRAQNADVVPSTPRIPPGT"
     gene            complement(1900..>2682)
                     /gene="prmB"
                     /locus_tag="VHE37_03800"
     CDS             complement(1900..>2682)
                     /gene="prmB"
                     /locus_tag="VHE37_03800"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVT34679.1"
                     /translation="DEAFHLVLHALKLPADLPAIYLESHVTARERADVLKLLRARVTT
                     RKPAAYLTGEIHFCGLAFKVDERVLVPRSPIGELIDRHFSPWLQQEPRNILDLCAGSG
                     CIGIACAMACPQAQVDLAELSPGALAVCRENIALHHLQERVRAVRSDLFGALAGKTYD
                     LIVSNPPYVPVAEWKALPEEFHREPRMALAAGADGMDIVARILRDAPAHMSENALLVC
                     EVGGSVEEFEARWPRLPVTWVEFERGGDGVFVISRSDLVKSL"
BASE COUNT          456 a          924 c          889 g          413 t
ORIGIN      
        1 aacctgcggg atcagcttgc gcatgttggc gaacatgcca atctccgcga tcacgcccag
       61 cgcccacagg aaacccgcct ggccgcgggt gtagccgtga cgctcgagga acagcgtgaa
      121 gaagttgtag tacggcgcga acgacagttg cgaggccagg cacaccgcca gcagcgcgag
      181 cacttccggg cggcgcagca cctcgcggat cgaaccctgc ggcgcatcgg cgtgatgcat
      241 caccggcgcg tcgggcacgc ggaaggtgga agccgccatc gccagccagg cgccgcccac
      301 cagccagggc aaggcgatga tgccggtgat gtccagcgca tagcccagcc ccagcacgga
      361 cacgatgaaa cccaccgaac cccacaggcg cacgcgggcg taatcgccgc cggacaggcc
      421 caggtgggtg agcgccaccg cttcgaactg cggcagcaac gcgtgccaga acacggtgta
      481 cgccagcatc agcgcgccca cccagaacac gccgtgcacg aacggaatgc ccatgaaggt
      541 gaccagcgac agcacgctgg cgatgcggat caggcgcacg cgccggccgc tgcggtcggc
      601 cagcgtgccc caggcaatgg gcacgaaggt gcgcatcatg cccatcagcg caaaggccac
      661 gcccatctgc gccgcagaga atccgcgcgc ggccaggtac ggcgaccaga acggcatgaa
      721 cgcgccgacg gtggcgtagt agaaaaaata aaaacccgcc aatctgtggg cgggtacgtg
      781 ctgagtcgcg gtactcatct cacgtgcccg gcgggatgcg cggggttgaa ggaacgacgt
      841 cggcgttctg cgcccgatgc cgcaggaagt gatccatcag taccagcgcc accatggcct
      901 cggcgatcgg cgtggcgcgc acgcccacgc agggatcgtg gcggccggtg gtgcgcacct
      961 gcgcgctgtg cccgtcctgg tcgatggtct gccccggcat gctcatgctg gaggtgggct
     1021 tcaccgccat gcgtgcccag atggtctggc cgctgctgat gccgccgaga attccgcccg
     1081 agtggttcga caggaagccc tgcggggaca tctcgtcgcg gtgctcgctg cccttcatgg
     1141 cggcgacctt catgccatcg ccgatctcaa cgcccttcac cgcgttgatc gacatcagcg
     1201 cgtaagcgag atcggcatcg aggcggtcat acaccggctc gcccaggcct ggcggcacgc
     1261 cgctggcctc gacgtagatc agcgcgccca ccgaatcgcc ctcgctgcgc atgcggtcga
     1321 tcagcgcttc cagttccggc acgcgttcgg gatcagcgca gaaaaacggg ttggcgttca
     1381 ccgcgctcca gtcgcggcgt tcgagcgtga tcgcgcccat ctgctccagg cagccgcgga
     1441 tgcgcacgcc gagtttttcg cgcaggtatt tcctcgcgat ggcgccggcc gccacgcgca
     1501 ccgcggtttc gcgcgcgctg ctgcgcccgc cgccgcgatg gtcgcggatg ccgtacttct
     1561 gcatgtaggt gtagtcggcg tggttgggcc ggaacacctg cttgatcttc tcgtagtcgt
     1621 agctgcgctg atccatgttg cggatcagca gcgcgatggg cgtgccggtg gtcctgcctt
     1681 caaacacgcc agagagaatc tcgaccttgt cctcttcctt gcgctgggtc acgtacttcg
     1741 acgtgccggg cttgcgccgg tcgagctcgg gctggatgtc ggtctccgac aattccagtc
     1801 ccggcgggca gccgtcgacc acgcagccga gcgcagcgcc gtggctttcg ccgaagctgg
     1861 tgacgcagaa cagcttgccg atggtgttgc cggacattgc tagagactct tcaccagatc
     1921 gctccgcgag atgacgaaca cgccgtcgcc gccgcgctcg aattccaccc aggtcaccgg
     1981 caggcgcggc cagcgcgcct cgaattcctc gacgctgccg ccgacctcgc acaccagcag
     2041 cgcattttcg ctcatgtgcg ccggcgcgtc gcgcaggatg cgcgcaacga tgtccatgcc
     2101 gtcggcaccc gcggccagcg ccatgcgcgg ctcgcggtgg aattcctcgg gcagcgcctt
     2161 ccattccgcc accggcacgt acggcggatt gctgacgatg aggtcatagg tcttgccggc
     2221 cagcgcgccg aacaggtcgg agcgcacggc gcgcacccgc tcctgcaggt gatgcagcgc
     2281 gatgttctcc ctgcacaccg ccagcgcgcc gggcgacagc tcggccaggt cgacctgcgc
     2341 ctgcggacag gccatcgcac aggcaatgcc gatgcagccg ctgccggcgc agagatcaag
     2401 gatgttgcgc ggctcctgct ggagccaggg cgagaagtgc cggtcgatca gttctccgat
     2461 cggcgagcgc ggcaccagca cgcgctcgtc gaccttgaac gccaatccgc agaaatggat
     2521 ttcgccggtg aggtaggccg ccggcttgcg cgtggtcacg cgcgcgcgca gcagcttgag
     2581 cacatccgcg cgctcgcgcg cggtgacgtg gctttcgagg tagatggccg gcaggtccgc
     2641 cggcagcttc agcgcgtgca gcaccaggtg gaacgcttcg tc
//
LOCUS       DASZZC010000174         4293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_270180,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000174 DASZZC010000000
VERSION     DASZZC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270180"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VHE37_03805"
     CDS             complement(<1..775)
                     /locus_tag="VHE37_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HVT34680.1"
                     /translation="MNKFELTAISRSLIARRWHVGLLPLVAPALVLANPSGGQVTAGS
                     AAITTPNSATTVINQASDAAIIQWQSFNVGRQEYVIFNQPTASAAVLNRVVGGDASLI
                     LGHMESNGRVFLINPMGVLFGKGAQVNVGSLVASTLDLADGDFLQGKYVFQSAPGSTP
                     AGVVNEGRIVAGQGGYVVLAGDYVKNAGVITAKLGDIALASGSAATLDMNGDGLVNFA
                     VSKAALSDRAGVDNIGKLVADGGTVVMTAQVARELMHAAV"
     gene            complement(940..2502)
                     /locus_tag="VHE37_03810"
     CDS             complement(940..2502)
                     /locus_tag="VHE37_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HVT34681.1"
                     /translation="MTPGQVQDTLKFPKPVEQPPPAVPVEKQHQPPPAAAAGRTVQVS
                     QFVFTGNTLYTQETLAALLSGYLNKPITLLDIYEAADKVTDFYSDHGYTLAAVNVPPQ
                     KLSGGTVRLDISEGVIGMVKVEGTSDARAYRIMNFLGDTQSGSIYHGPQLEAGLQNVN
                     QLPGLSARAVVQPGREYGTTDLVIKTEEKPLTGNVFVDNYGTQAIGQTRVAANVILNN
                     PARIEDQLNLLVLRSSGGRLQYEYAGYSLPLNYAATRLNLAYGHARFEVPGSVEGSND
                     NGKVEVAQPLLRTRTDRLDVSAAVSRTNSNAEISGTGLPLSGTSITLLELNTAYNHVY
                     RNYAVTQLTANIATNFDHANTAAEVRPVAAKASNADQRFRLEIDAQQLQPLVEKYFLL
                     AHLNGEWSPDPLSNATQYSIGGPQSVRAYATSEVRGDQGYLAQLTLGRNIDAGPVKFT
                     GRLFADSGRVFCADASPDCKAESLSSAGVGLDAAWWHLSGKVDYSLPIDPRTPSDGHH
                     DGRLFGSLFVNF"
     regulatory      2880..2973
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3109..4191
                     /gene="gcvT"
                     /locus_tag="VHE37_03815"
     CDS             3109..4191
                     /gene="gcvT"
                     /locus_tag="VHE37_03815"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HVT34682.1"
                     /translation="MLKTTALHGEHVKLGARLVDFAGWEMPIQYASQLDEHHAVRGGA
                     GMFDVAHMCAVDLHGARTREFLRRLLANDVAKLTLPGKALYTCMLREDGGIIDDLIAY
                     FISEDFFRLVVNAGTADKDIAWLQRHATSFGVEVKPRRDLGILAVQGPQAREKVAALL
                     PQPLGAKSLELIAFQAAWQGEWFVGRTGYTGEDGFEIIVPNAELPGLWQKLLAAGVKP
                     AGLGARDTLRLEAGMNLYGNDMDESTHPLESGLGWTIAWEPKDRDFIGRSAIEKFRGA
                     SPKKFVGLLLEGRGVLRAHMKVRFGGGGSGETTSGGFAPTLKNSIAFARVSGDTSGAC
                     EVEIRGQWVAARVVKPPFARNGKSLV"
BASE COUNT          762 a         1346 c         1419 g          766 t
ORIGIN      
        1 tcaccgcggc gtgcatcagt tcgcgcgcga cctgcgcggt catcaccacg gtgccgccgt
       61 cggccaccag cttgccgatg ttgtcgacgc cggcgcggtc ggacagcgcg gccttgctca
      121 ccgcgaaatt gaccaggccg tcgccattca tgtcgagcgt ggcagccgag ccggaggcca
      181 gcgcgatgtc gccgagcttg gccgtgatga cgccggcgtt cttcacgtaa tcgcccgcca
      241 gtaccacgta gccgccctga ccggcgacga tgcgcccttc gttgaccacg ccggccggcg
      301 tgctgccggg cgcggactgg aacacgtact tgccctgcag gaaatcgccg tcggccaggt
      361 ccagcgtcga ggccaccagg ctgccgacgt tgacctgcgc acccttgccg aacagcactc
      421 ccatcgggtt gatcaggaac acccggccgt tggattccat gtggccgagg atcaggctgg
      481 catcgccgcc gaccacgcgg ttgagcaccg ccgccgaggc ggtgggctgg ttgaagatca
      541 cgtattcctg gcggccgacg ttgaagctct gccactggat gatggcggcg tcgctggcct
      601 ggttgatcac cgtggtcgcc gaattcggcg tggtgatggc ggcgctgccg gcggtgacct
      661 ggccgccgga aggattggcc agcactagcg cgggcgccac cagcggcagc aagccgacat
      721 gccagcgacg cgcaatgagg gaacgtgaaa tcgcggtcag ctcaaatttg ttcatggcgg
      781 gcaccagtga ctcggaaaaa ctcaccgcgc gtccgcaaca gccgggccgc ggcgcggtta
      841 acagcttgaa aaattatatg tttcgcaacg actaaaaatg tgtgacggtg ttgggcattc
      901 gaggcaactg cggatcggtc tggccgcgtg cggcgcgtct cagaaattga cgaacagcga
      961 gccgaacaga cgcccgtcgt ggtggccatc gctgggcgtg cgtggatcga tcggcaggct
     1021 gtagtcgacc ttgccgctca ggtgccacca ggccgcgtcg agcccgacgc cagcgctgct
     1081 caggctttcc gccttgcaat cgggcgaagc gtcggcgcag aacacgcggc ccgagtcggc
     1141 gaacaggcgc ccggtgaact tgaccggacc ggcgtctata ttgcgaccca gcgtgagctg
     1201 cgccaggtaa ccctggtcgc cgcgaacttc ggaagtggcg tacgcgcgca cgctctgcgg
     1261 tccgccgatg gaatattgcg tggcgttgga cagcggatcg ggcgaccact cgccgttgag
     1321 gtgcgcaagc aggaaatatt tttcgaccag cggctgcagc tgctgcgcgt cgatttccag
     1381 acggaagcgc tggtcggcat tgctcgcctt cgcggccacc ggccgcactt ccgcggcagt
     1441 gttcgcatgg tcgaagttgg tggcgatgtt ggccgtcagc tgtgtgaccg cgtagttacg
     1501 gtacacgtgg ttgtaggcgg tgttgagttc cagcagcgta atcgaggtgc cgctgagcgg
     1561 cagcccggtg ccggagatct cggcgttgga gttggtgcgc gagacggcgg cggatacgtc
     1621 gagccggtcg gtgcgcgtgc gcagcagcgg ctgcgcaact tccaccttgc cgttgtcatt
     1681 gctgccttcg accgagccgg gcacttcgaa gcgcgcatgc ccgtaggcaa ggttcaggcg
     1741 ggtggcagcg tagttcagcg gcaggctgta gccggcgtac tcgtattgca gccggccgcc
     1801 gctcgaacgc agcaccagca ggttgagctg gtcttcgatg cgcgcgggat tgttgaggat
     1861 gacattggcc gccacgcggg tctgcccgat tgcctgggtg ccgtagttgt cgacgaacac
     1921 gttgccggtc agcggctttt cctcggtctt gatcaccagg tcggtggtgc cgtactcgcg
     1981 gcctggctgg accaccgccc gcgccgacag ccccggcagc tggttcacgt tctgcaggcc
     2041 ggcttcgagt tgcgggccgt ggtagatgct gccgctctgc gtgtcgccga ggaaattcat
     2101 gatgcggtag gcgcgcgcgt cgctggtgcc ctccaccttc accatgccga tcacgccttc
     2161 gctgatgtcc agccgcacgg tgccgcccga cagcttctgc ggcggcacgt tgaccgcggc
     2221 cagcgtgtag ccatggtcgg agtagaaatc cgtaaccttg tcggccgctt cgtagatgtc
     2281 gagcagagtg atcggtttat tcaaatatcc gctcagcagc gcagccagcg tctcctgcgt
     2341 gtagagcgtg ttgccggtga acacgaactg gctcacctgc acggtgcggc ccgcggcggc
     2401 ggcgggcggc ggctggtgct gcttttccac cgggaccgcc ggcggcggct gctccaccgg
     2461 cttgggaaac ttgagcgtgt cctgcacctg gcccggcgtc acctgggcgc aaactgaact
     2521 gctgatctga agtgccgcga gagccagaag gatttttaga ttcttattcg acccgcgcat
     2581 cggcattgct cccaggttat ttggcatctt gccgccgacc acattgtgcc gggcgactat
     2641 agcccaacga ttcttcacct tccaacggcg cgcctgacga aaacttctcc attgcgaagt
     2701 gcatatcagt ttcggcattt ggcaaaaaaa acagcttacc gtacccggtt ttccgatgtt
     2761 ataaacaata taataaataa aaaatagata aatattttat aacacgatcg gaaggggtgc
     2821 ggtggcccca aaaaatgcct cgccagtgca cgcgactgcc ggtatagtgc gcgccgggct
     2881 ttatcttcac gcgagagtcc gccggaaaat cggcggcacc gaaggcgcaa aatccgctca
     2941 gaatcgctca ggtaaaagga gcgtgaagtc gggcccactc tggagagttg ccgcgcgagc
     3001 ggcacaccga tggggcagga gcgcggcact tccggtgccc gctctaatct ctcaggtacc
     3061 caagacagag gagcggtttt cgcaatgaac catcccgcgg gaaccgcaat gctcaagaca
     3121 accgctctgc acggcgaaca cgtcaagctc ggcgcacgcc tggtcgactt cgccggctgg
     3181 gaaatgccga tccagtacgc ctcgcagctc gacgagcacc acgccgtgcg cggcggcgcc
     3241 ggcatgttcg acgtggcgca catgtgcgcg gtcgacctgc atggcgcgcg cacgcgcgag
     3301 ttcctgcgcc ggctgctggc caatgacgtg gccaagctca cgctgcccgg caaggcgctc
     3361 tatacctgca tgctgcgcga ggacggcggc atcatcgacg acctcatcgc atatttcatc
     3421 agtgaggatt tcttccgcct ggtggtcaac gccggcaccg ccgacaagga catcgcctgg
     3481 ctgcaacggc acgcgacgtc cttcggtgtc gaggtgaagc cgcggcgcga cctcggcatc
     3541 cttgcggtgc agggcccgca ggcgcgcgag aaagtggccg cgctgctgcc tcagccgctg
     3601 ggcgcgaagt cgctcgagtt gatcgcgttc caggcggcct ggcagggcga gtggttcgtc
     3661 ggccgcaccg gctacaccgg cgaggacggt ttcgaaatca tcgtgcccaa cgccgaactg
     3721 cccgggctgt ggcagaagct gctcgcggcc ggcgtgaaac ccgcgggcct gggcgcgcgc
     3781 gacacgctgc gcctggaagc cggcatgaat ctctacggca acgacatgga cgagtccacc
     3841 catccgctcg agtcggggct gggctggacc attgcgtggg aacccaagga ccgcgatttc
     3901 atcggccgca gcgccatcga aaaattccgc ggcgccagtc cgaagaaatt cgtcggcctg
     3961 ctgctggaag gccgcggcgt gctgcgcgcg cacatgaaag tgcgctttgg cggtggcggc
     4021 agcggcgaaa ccaccagcgg cggttttgcg ccgacattga agaattccat tgccttcgcg
     4081 cgcgtgtccg gcgacaccag cggcgcctgc gaggtcgaga tccgcggcca gtgggttgca
     4141 gcgcgcgtgg tcaagccgcc gttcgcgcgc aacggcaagt cgctggtctg aacatcaccc
     4201 cattcaaata acggagcaca catgagcaac gtcccggctg atctcaagta cgtgaagagc
     4261 cacgagtggg tgaagaagca ggccgacggc agc
//
LOCUS       DASZZC010000175         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_592583,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000175 DASZZC010000000
VERSION     DASZZC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592583"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /gene="ftsH"
                     /locus_tag="VHE37_03820"
     CDS             complement(<1..1366)
                     /gene="ftsH"
                     /locus_tag="VHE37_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HVT34683.1"
                     /translation="MNDLAKNLLLWVVILVVLMSVFQSFSGRSHATQQLVYSEFLNQV
                     KQGNVAEVQIEGATIHGDLKNGQHFTAVSPETDNRGMVQTLIDNNVKFDGELPKETPL
                     IVQLLINAFPILLLIAVWVYFMRQMQGGAGGRGAMSFGKSRARMLNADQVKVTFADVA
                     GVEEAKQEVAELVDFLKDPGKFQKLGGKIPRGVLMVGSPGTGKTLLAKAIAGEAGVPF
                     FTISGSDFVEMFVGVGASRVRDMFDQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNEGVIIIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQ
                     ILKVHMRAVPLHDGVKPEILARATPGFSGADLANLVNEAALFAARANKRLVDQDDFEK
                     ARDKIIMGTERRSLVMSEEEKRNTAYHEAGHAIIGVLVPDHDPVYKVTIIPRGRALGV
                     TSYLP"
     gene            complement(1473..2120)
                     /gene="rlmE"
                     /locus_tag="VHE37_03825"
     CDS             complement(1473..2120)
                     /gene="rlmE"
                     /locus_tag="VHE37_03825"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HVT34684.1"
                     /translation="MSKKRTASSARWLAEHESDIYVQKARALGYRSRAVFKLEEIQQK
                     DRLLKPGQVVVDLGAAPGGWSEFARPLLGSKGTLIALDILPMDPIAGVDIILGDFREE
                     SVLRQLEEKVGRHAVDLVLSDMAPNISGVATADQASSIYLCELALHFAQEHLQPQGAF
                     VVKVFQGEGSEAYLKAVRQAFVQVVIRKPKASRPRSREVYLVARGLKEPRSRQPF"
BASE COUNT          369 a          720 c          675 g          389 t
ORIGIN      
        1 cgggcaggta cgaggtgaca cccagcgcgc ggccgcgcgg gatgatggtg accttgtaaa
       61 ccgggtcatg gtccggcacc agcacaccga tgatggcgtg gccggcctcg tggtacgcgg
      121 tgttgcgctt ctcttcctcg ctcatgacca gcgagcggcg ctcggtgccc atgatgatct
      181 tgtcgcgcgc cttctcgaaa tcatcctggt caaccaggcg cttgttggcg cgcgcggcga
      241 acagcgcggc ctcgttgacc aggttggcca ggtcggcgcc ggagaaaccg ggcgtggcgc
      301 gcgccaggat ttccggcttg acgccgtcat gcagcggcac cgcgcgcatg tgcaccttga
      361 gaatctgctc gcggccgcgc acgtcgggca gcggcaccac tacctggcgg tcgaagcggc
      421 ccgggcgcag caacgcggga tcaagcacgt ccggccggtt ggtggcggcg atgatgatca
      481 cgccctcgtt gccctcgaag ccatccattt cgaccagcaa ctggttcagc gtctgctcgc
      541 gctcgtcgtg accgccgccc aggccggcac cgcgatggcg gccaaccgcg tcgatctcat
      601 cgatgaagat gatgcacggc gcgtgcttct tggcctggtc gaacatgtca cgcacgcgcg
      661 aggcgcccac gccgacgaac atctcgacga aatccgaacc ggagatggtg aaaaacggca
      721 caccggcctc gccggcgatg gccttggcca gcagcgtctt gccagtgccg ggcgagccca
      781 ccatcagcac gccgcgcgga atcttgccgc ccagcttctg gaacttgccc gggtccttga
      841 ggaagtcgac cagctcggcc acttcctgct tggcttcttc cacgccggcc acatcggcga
      901 aggtgacctt gacctgatcg gcgttgagca tgcgcgcgcg gctcttgccg aagctcatgg
      961 cgccgcggcc gccggcgccg ccctgcatct ggcgcatgaa gtacacccac accgcgatca
     1021 gcagcaggat ggggaaggcg ttgatcagca gttgcacgat cagcggcgtt tccttgggca
     1081 gctcgccgtc gaacttgacg ttgttgtcga tcagcgtctg caccatgccg cggttgtcgg
     1141 tttccggcga gacggcggtg aagtgctggc cgttcttgag atcgccgtgg atggtggcgc
     1201 cctcgatctg cacctcggcc acattgccct gcttgacctg gttgaggaac tccgaataga
     1261 ccagctgctg ggtcgcgtgg ctgcgcccgg aaaagctctg gaagacactc atcagcacga
     1321 cgagtatcac cacccataac agcagattct tggccagatc gttcaactta gttccttcct
     1381 tgcacccgcc tgcaaaccac cttacaccag ggctatcccg ccctcaataa acaaattctg
     1441 gtcgctgcgt caggtgacgc aagtctcatc ctttagaaag gctggcgcga cctgggttcc
     1501 ttcagtccgc gcgccaccag gtacacctcg cgcgagcgcg gacgcgaggc cttgggcttg
     1561 cggatcacca cctggacgaa agcctgccgt accgccttaa ggtatgcctc cgatccttcg
     1621 ccctggaaca ccttcaccac gaaagcgccc tgcggctgca ggtgctcctg ggcaaaatgc
     1681 agcgccagct cgcacaggta gatgctggaa gcctggtcgg cagtggccac acccgagata
     1741 ttgggggcca tgtcggacaa aacaaggtcc accgcgtggc ggccgacttt ttcctccagt
     1801 tgccgcagca ccgactcctc gcggaaatcg cccaggatga tgtcgacgcc ggcgatcggg
     1861 tccatcggca ggatgtccag cgcgatcagc gtgcccttgg agccgagcag cgggcgcgcg
     1921 aattcgctcc agccgccagg cgccgcgccc aggtccacca cgacctgacc cggcttgagc
     1981 agccggtcct tctgctggat ttcctcgagc ttgaacaccg cgcgcgagcg atagcccagc
     2041 gcacgcgcct tctgcacgta gatgtcggat tcgtgctcgg cgagccagcg ggcggagctt
     2101 gcggtgcgtt tcttgctcat gatcggcgtt gtaggagcgc gccttgcgcg cga
//
LOCUS       DASZZC010000176         9365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_205954,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000176 DASZZC010000000
VERSION     DASZZC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205954"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VHE37_03830"
     CDS             complement(<1..1064)
                     /locus_tag="VHE37_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVT34685.1"
                     /translation="MAAVDDDIRADFLVEAGELVQRLPEQLMQLEQQPEDIALLNAIF
                     RAFHTVKGGAGFLEYTAMVELCHATEDLFNLLRNGKRGVTPELMDVVLKSADHLAQQM
                     REVVEGGAPAAAPAELLQALHALCSDEKPKAKARAKGKEKEKAAQPQLPLAPAAQSSL
                     EKVGDGAGAVSKTSDTISEDEFEALLDQLQAEKKNPPAAEIPQPVPAPLAGEAAAAPS
                     TTRTEARPAAVAASPAPAETTIRVDTHRLDKLMNLVGELVLVRNRLRTLRGHNDAAAL
                     AKTVANLDFITRDLQGAVMQIRMQPVKKVFSRFPKVARDLARQLGKQVEVTLVGEDTD
                     LDKNLVEALADPLVHMVRNA"
     gene            complement(1075..1815)
                     /locus_tag="VHE37_03835"
     CDS             complement(1075..1815)
                     /locus_tag="VHE37_03835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HVT34686.1"
                     /translation="MEPAFVEASAPDARAHLLDRLRALTALLEAGDDAGVEHGLAELL
                     RERDDGLYRHLGRITRELHRAVADLKFDDQLAHLAKAEMPDAASRLDHVIEMSEKAAH
                     RTLDLVEQSRTLASRIAESEVDLAFVHQRMQLCVRTPGACNVVANEIGLVRSSIKSCA
                     QQLRENLSSLAQAQEYQDLSGQVIRRVITLVRSVESTLIALLQATGSNLNGVTPAAPQ
                     GLAGPVIAGINTGATQQDADALLAELGF"
     gene            complement(1818..2219)
                     /locus_tag="VHE37_03840"
     CDS             complement(1818..2219)
                     /locus_tag="VHE37_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HVT34687.1"
                     /translation="MDKNMKILIVDDFSTMRRIVKNLLNDLGYTNTTEADDGNTAWPM
                     LQAGDFQFVVTDWNMPGMTGIDLLKHIRGDARLAALPVLMVTAEAQRDQIIEAAKSGV
                     NGYIIKPFTAVTLKEKMDKIFQRMAETTAAA"
     gene            complement(2246..2980)
                     /locus_tag="VHE37_03845"
     CDS             complement(2246..2980)
                     /locus_tag="VHE37_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HVT34688.1"
                     /translation="MSSARPTTSTAATERELVARHAELVKRIAYHLAARLPASVEVDD
                     LIQAGVIGLLEAARHYNGDRGASFETYAGIRIRGAMVDELRKSDWAPRSVHRRAREAA
                     AVISDIEQHSGREARETEIAEKLGIPLEEYRQIAFDAARCHVLSFDAHADEDGAPIDL
                     PSDGPGPSDELARDEFQRELARAIGDMPEREKQVLAMYYQDEMNLREIGAVLEVSESR
                     ACQIHGQALLRLRARMHEWRSEAIHE"
     gene            complement(2977..3816)
                     /locus_tag="VHE37_03850"
     CDS             complement(2977..3816)
                     /locus_tag="VHE37_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HVT34689.1"
                     /translation="MRSHKPVRVIAVTGGKGGVGKTTVSVNLSVAMALSGKKVMLLDG
                     DLGLANVDVMLGLQPTYNLAHVIEGRCTLEDTLLAGPGGLLVVPAASGKRQMAELSKS
                     QHAGLVQAFSELQRPLDTLVVDTAAGIADSVITFTQASQEVIVVLCNDPSSMTDAYAL
                     VKVLSREHGIDRVHVLSNMVHNATEAREMFDSLRRVAERFLDVTLDYMGSVPHCEWLK
                     RAVRKQRAVVDAYPNSPSAAAYMALAKKADTWSLPRGARGNLEFFVERLVNSAGPAVG
                     VAA"
     gene            complement(3842..5167)
                     /gene="flhF"
                     /locus_tag="VHE37_03855"
     CDS             complement(3842..5167)
                     /gene="flhF"
                     /locus_tag="VHE37_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HVT34690.1"
                     /translation="MKIKRFEAGSMREALRLVRDEQGPDAVILSNRRTANGVEVVAAV
                     DYDAALMQQGLRHAQVQSPLPAAPQNESAPAPFARVHDPRAPLAPRIDEAPKPAKSVT
                     TPVRDELQQARATIAAKAAAIRKRRGAAVLAKKKLSSAQPAPVPRLVAPQLAPAPVVR
                     AAPVPMAAHQRDAAQQMLMQFGLDRALINEILQQMPADTQAERARFLPLALLARRIAV
                     PEDEALMSGGIYALLGPTGAGKTTTLAKLAARYIAQHSARDVAIVSTDHYRIGAQEQL
                     YTYGRLLGLPVHSAANAEELKSTLLRLAGKKLVLIDTAGMSARDTKLAGQFAALTGSG
                     VPIKPLLVLAANSAPHDLDLVVQRFSAIHPHACVLTKLDESTRLGGALSVVIRRQLPV
                     AYTANGQRVPEDLALARADRLVLSAAQLARAAAPAPIKKISNGAVHAAH"
     gene            complement(5184..7250)
                     /gene="flhA"
                     /locus_tag="VHE37_03860"
     CDS             complement(5184..7250)
                     /gene="flhA"
                     /locus_tag="VHE37_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HVT34691.1"
                     /translation="MTAESPWQRLRAVGAGGLGAPLLLLVILAMMVVPLAPLALDVLF
                     TFNIALSIVILLAVVYVMRPLDFSAFPSVLLLVTLLRLALNVASARVVLLHGHEGSSA
                     AGHVIEAFGEFVIGGHYSVGFIVFIILTIINFMVVTKGAERVSEVSARFVLDALPGKQ
                     MAIDADLNAGLLTREDARERRNEVREEADFYGSMDGASKFIRGDAMAGILILIINIGG
                     GLAVGTLEHGLPLSAALKNYTLLTIGDGLVAQIPALLLSTAVAMLVTRMSRSSELGQQ
                     VMGQVFKDPRVLVITAGILGLLGVIPGMPNLVFLALAAATGAAAYFMKQKAQQTTEAA
                     AQPQPAPAQPPKELSWDDVSADDALGLEVGYRLIPMVDARQGGELMSRIKGVRKKLTQ
                     ELGFLIPPVHIRDNLELQPGHYRITLHGVPLASGQVYPERDLALNPGRVYGTLEGIET
                     RDPAFGMPAVWIEKGAREHAATLGYSVVDASTVVATHLSHLVKEHCSELLGFDEAQQL
                     LTALAKSSPKLVEDLVPKVLPLGVFVKVLQNLLAERVPIRNLRIIAEAIAEAAPRSQD
                     PVALAGAARVALGRQIVQEINGTDAELPVLTLAPALERVLQDSLQSGGAVLEPGLAER
                     MHKSISDNAARQESSGQPAVLLVPGPLRPLLAKFTRQTVPGLHVLAFNEVPESKKLRM
                     VGAVGY"
     gene            7520..>9365
                     /locus_tag="VHE37_03865"
     CDS             7520..>9365
                     /locus_tag="VHE37_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HVT34692.1"
                     /translation="MNRLSPGLLLLATLPAFAADQAPELAVRFPHLYVEYRLGDDGAA
                     VETRDWAMTVLQQRAIEQAKQSSITYSTSIQHAEVLAAWTEKADGRRIDAPKSNFQLE
                     ANSGNDRNAPVFSDQTTLTVVFPEVEVGDTVHFNYRVSESEPMFPGQFSDMEIFSRST
                     AYDDVRVSLDAPAALWLQTQANQMQAQPAVEKDGRRILAWTYANPNPARDKRRDYSVF
                     DIESAPGVLFSTFHDYAAIAQAYAARARPKALPDERVRRLAADIVGGRTDAREQAHAL
                     YDWVATNITYGGNCIGTGAVVPHDLSFVLDNKMGDCKDHATLLQALLAARGIEATQAL
                     VNAGSGYRLPKVPVVSYVNHVINYLPAFGIYADSTSQDTPFGLLPFADADKPVLLVDG
                     KDTVGHTPASAAGRRHQDMKSVLTIAADGSVSGEVEIHLGGALAAAARAGMRGFPKDE
                     EADLVEHMLQRRGYIGKGSFEHDDPKDLTDSYRYRISFQMQDYTQLPGPAGFVIAPVI
                     SSSAPLGEFASTAVADDVDYDTTCSGASSVEEYSYQLPANMKVLATPDDVELSNGFLH
                     YRAHYHLAGRRLEVRREIDDRTPGNVCGPEISAAYKKFAKGMLRDLKAQ"
BASE COUNT         1440 a         3165 c         3185 g         1575 t
ORIGIN      
        1 atggcgttgc gcaccatgtg caccagcgga tcggccagcg cctccaccag gttcttgtcc
       61 aggtcggtgt cctcgccgac cagcgtcact tccacctgct tgcccagctg gcgggcgaga
      121 tcgcgcgcca ctttcgggaa acgcgaaaac accttcttca ccggctgcat gcggatctgc
      181 atcaccgcgc cctgcaggtc gcgcgtgatg aaatcgaggt tggccacggt cttggccagc
      241 gccgccgcgt cgttgtggcc gcgcagcgtg cgcaggcggt tgcgcacgag tacgagttcg
      301 ccgaccaggt tcatcagctt gtcgagacga tgggtgtcga cgcggatggt ggtttcggca
      361 ggtgcgggtg atgcggcaac tgcagcgggc ctggcttcag tcctggttgt ggacggcgcg
      421 gccgctgcct ctccggcaag gggagcggga accggttggg gaatttcagc ggctggcgga
      481 tttttcttct ccgcctgcag ctggtcgagc agcgcttcga attcgtcttc gctgatggtg
      541 tcggacgttt ttgaaacggc cccggcgccg tccccaactt tttccaggga ggattgggca
      601 gcgggggcca gcggcaactg cggctgcgca gccttttcct tttccttccc tttcgccctg
      661 gctttcgcct tcggcttctc atccgagcac agcgcgtgca gcgcctgcag cagttcggcc
      721 ggcgcggccg ccggcgcgcc gccttccacc acctcgcgca tctgctgcgc caggtgatcg
      781 gcagacttca gcaccacgtc catcagttcc ggcgtcacgc cgcgcttgcc gttgcgcagc
      841 aggttgaaca ggtcctcggt ggcatggcac agctccacca tcgcggtgta ttcgagaaag
      901 ccggcgccgc ccttcaccgt gtggaaggcg cggaagatgg cgttgagcag cgcgatatct
      961 tccggctgct gctccagctg catcagctgc tcgggcagtc gctgcaccag ttccccggcc
     1021 tcgaccagga aatcggcgcg gatgtcgtcg tcgacggcgg ccatgtgtgt cgtcctagaa
     1081 acccagttca gccagcagcg cgtcggcgtc ctgctgggtg gcaccggtgt tgatgccggc
     1141 gatcaccggc ccggccaggc cctgcggcgc ggcgggagtc acaccgttga ggttgctgcc
     1201 ggtggcctgc agcagggcga tcagcgtgga ctccacgctg cgcaccaggg tgatcacgcg
     1261 gcggatcacc tggcccgaca ggtcctggta ttcctgcgcc tgcgccagcg aggaaagatt
     1321 ctcgcgcagc tgctgcgcgc agctcttgat gctggagcgc accagcccga tctcgttggc
     1381 caccacgttg cacgcgccgg gcgtgcgcac gcacagctgc atgcgctggt gcacgaaggc
     1441 caggtccacc tcgctctcgg cgatgcgcga ggccagcgtg cggctctgct cgaccaggtc
     1501 gagcgtgcgg tgcgcggcct tctccgacat ctcgatcaca tggtccaggc gcgacgccgc
     1561 atccggcatt tccgccttgg ccaggtgcgc cagctggtcg tcgaacttga ggtcggccac
     1621 cgcgcggtgc agctcgcggg tgatgcgccc gagatggcgg tagaggccat cgtcgcgttc
     1681 gcgcagcagt tcggccaggc catgctcgac gccggcgtcg tcgccggcct cgagcagcgc
     1741 ggtcagcgcg cgcaggcggt cgagcaggtg cgcgcgcgcg tcgggcgcgg acgcttccac
     1801 gaatgcgggc tccatgttca tgccgccgcg gtggtttcgg ccatgcgctg gaagatcttg
     1861 tccatcttct ccttcagcgt caccgcggtg aacggcttga tgatgtagcc gttcacgccg
     1921 gacttggcgg cctcgatgat ctggtcgcgc tgtgcctcgg cagtgaccat cagcaccggc
     1981 agcgcggcca gacgcgcatc gccgcggatg tgcttgagca ggtcgatgcc ggtcatgccg
     2041 ggcatgttcc agtcggtgac cacgaactgg aaatcgccgg cctgcagcat cggccacgcg
     2101 gtgttgccgt cgtcggcctc ggtggtgttg gtgtaaccca ggtcattgag caggttcttg
     2161 acgatacggc gcatggtcga gaagtcgtcg acgatcagga tcttcatgtt cttgtccatc
     2221 agttcactcc gcgttcaatc ccggcttatt catggatggc ttcgctgcgc cactcgtgca
     2281 tgcgcgcacg cagccgcagc agcgcctgcc cgtggatctg gcaggcgcgc gattcggaaa
     2341 cctcgagcac cgcgccgatc tcgcgcaggt tcatctcgtc ctggtagtac atggccagca
     2401 cctgcttctc tcgctcgggc atgtcgccga tggcgcgcgc cagctcgcgc tggaactcgt
     2461 cgcgggccag ctcgtcggac ggcccggggc cgtcggacgg caggtcgatc ggcgcgccgt
     2521 cctcgtcggc gtgcgcgtcg aacgacagca cgtggcagcg cgcggcatcg aaggcgatct
     2581 gccggtattc ctccagcggg atgcccagct tctcggcgat ctcggtctcg cgcgcctcgc
     2641 ggccgctgtg ctgctcgatg tcgctgatga cggcggcggc ttcgcgtgcg cggcggtgca
     2701 ccgagcgcgg cgcccagtca ctcttgcgca gctcgtcgac catggcgccg cggatgcgga
     2761 tgccggcgta ggtttcgaac gaggcgccgc ggtcgccgtt gtagtggcgc gcggcttcca
     2821 gcaggccgat gacgccggcc tggatcaggt catcgacttc cacgctggcc ggcaggcgcg
     2881 ccgccaggtg gtaggcgatg cgcttgacca gttcagcgtg gcgcgcaacc agctcgcgct
     2941 ccgtcgccgc ggtggacgtg gtggggcgcg cgctgctcac gccgccaccc cgaccgcggg
     3001 cccggcgctg ttgaccagcc gctcgacgaa gaattcgaga ttgccgcgcg cgccgcgcgg
     3061 caggctccag gtgtcggcct tcttggccag cgccatgtag gcggcggccg aaggcgagtt
     3121 gggataggca tcgaccaccg cgcgctgctt gcgcaccgcg cgcttgagcc attcgcaatg
     3181 cggcaccgag cccatgtagt cgagcgtcac gtcgaggaag cgctcggcca cgcggcgcag
     3241 gctgtcgaac atctcgcgcg cctcggtggc gttgtgcacc atgttgctca gcacgtgcac
     3301 gcggtcgatg ccgtgctcgc gcgacagcac cttgaccagc gcgtaggcgt cggtcatcga
     3361 tgacgggtcg ttgcacagca ccacgatcac ttcctgcgac gcctgggtga aggtgatgac
     3421 gctgtcggca atgccggcgg cggtgtccac caccagcgtg tcgagcggac gctgcagctc
     3481 ggagaaggcc tgcaccagtc cggcgtgctg gctcttcgac agttccgcca tctgccgctt
     3541 gcccgatgcg gcgggcacca ccagcaggcc gccggggccg gcgagcagcg tgtcctccag
     3601 cgtgcagcgg ccctcgatga cgtgcgccag gttgtaggtg ggctgcaggc cgagcatgac
     3661 gtcgacgttg gccaggccaa ggtcgccgtc gagcagcatc accttcttgc ccgacagtgc
     3721 catggccacc gacaggttga ccgacaccgt ggtcttgccc acgccgccct tgccgccggt
     3781 gacggcgatg acgcgcaccg gcttgtgcga ccgcaggttg cgcaggccgg cggcctggtc
     3841 ttcagtgcgc tgcatgtacg gctccgttgg aaatcttctt gatgggtgcc ggcgcggcgg
     3901 cgcgcgccag ctgcgcggcg gacagcacca gcctgtcggc ccgcgccagc gccaggtctt
     3961 cgggcacgcg ctggccgttg gcggtgtagg cgaccggcag ctggcggcgg atgaccaccg
     4021 acagcgcgcc gcccaggcgc gtgctctcgt cgagcttggt cagcacacac gcatgtggat
     4081 ggatggcgga gaaacgctgc accaccaggt cgaggtcgtg cggcgcgctg ttggcggcca
     4141 gcaccagcag cggcttgatc ggcacaccgc tgccggtcag cgcggcgaat tgcccggcga
     4201 gcttggtgtc gcgcgcgctc atgccggcag tgtcgatcag caccagcttc ttgccggcca
     4261 ggcgcagcag ggtggacttg agttcctcgg cattggccgc gctgtgcacc ggcaggccga
     4321 gcaggcggcc gtaggtgtac agctgctcct gcgcgccgat gcggtagtgg tcggtggaaa
     4381 cgatggccac gtcgcgcgcc gagtgctgcg cgatgtagcg cgcggcgagc ttggccagcg
     4441 tggtggtctt gccggcgccg gtcggcccga gcagtgcgta gatgccgccg gacatcagcg
     4501 cctcgtcctc cggcacggcg atgcgccgcg ccagcaacgc cagcggcagg aagcgcgcgc
     4561 gctcggcctg cgtgtcggcg ggcatctgct gcaggatctc gttgatcaat gcgcggtcga
     4621 gcccgaactg catcagcatc tgctgcgcgg cgtcgcgctg gtgtgcagcc atgggcaccg
     4681 gggcggcgcg caccaccggc gcgggcgcca gctgcggcgc gaccaggcgc ggcaccggcg
     4741 cgggctgcgc cgatgacagc ttctttttcg ccagcacggc cgcaccgcgg cgcttgcgga
     4801 tggcggcggc cttggcggcg atggtcgcgc gcgcttgctg cagttcatcg cgcaccggtg
     4861 tcgtcaccga cttcgccggc ttcggcgctt cgtcgatgcg cggcgccagc ggcgcgcgcg
     4921 gatcgtgcac gcgcgcgaac ggcgcgggcg cagattcatt ttgcggcgcg gccggcagcg
     4981 gcgactgcac ctgcgcatga cgcaggccct gctgcatcag cgcggcgtcg tagtcgaccg
     5041 cggccaccac ttccacgcca ttggcggtgc ggcggttgga caggattacc gcgtcggggc
     5101 cctgctcgtc gcgcaccagg cggagcgcct cgcgcatgct gcctgcttcg aatcgcttga
     5161 tcttcattta tctgttgccc tgctcagtag cccaccgcac cgaccatccg cagcttcttg
     5221 ctctcaggca cttcgttgaa cgccagcacg tgcagccccg gcaccgtctg ccgcgtgaac
     5281 ttggccagca gcggccgcag cggtccgggc accagcagca ccgcgggctg tccggaagat
     5341 tcctggcggg ccgcgttgtc gctgatcgac ttgtgcatgc gctcggcgag tccgggttcc
     5401 agcaccgcac cgcccgattg caggctgtcc tgcaacacac gttccagcgc cggtgccagc
     5461 gtgagcacgg gaagctccgc atcggtaccg ttgatctcct gcacgatctg ccggcccagg
     5521 gcgacgcgcg cggcgccggc cagcgcgacg ggatcttgac tgcgcggtgc ggcctcggcg
     5581 atcgcctcgg cgatgatgcg caggttgcgg atcggcacgc gctcggccag caggttctgc
     5641 agcactttca cgaacacgcc cagcggcagg actttcggca ccaggtcctc gaccagcttg
     5701 ggtgagctct tggccagcgc ggtgagcagc tgctgcgcct cgtcgaagcc cagcagctcc
     5761 gagcaatgct ccttgaccag gtgcgacagg tgcgtggcga ccacggtgct cgcgtcgacc
     5821 acgctgtagc ccagcgtggc cgcgtgctcg cgcgcgccct tctcgatcca caccgccggc
     5881 atgccgaacg cggggtcgcg cgtctcgatg ccttcgagcg tgccgtaaac gcggccgggg
     5941 ttgagcgcga gatcgcgctc cgggtagacc tgcccgctgg ccagcggcac gccgtgcagc
     6001 gtgatgcggt agtggcccgg ctgcagctcg aggttgtcgc ggatatgcac cggcggaatc
     6061 aggaagccga gttcctgcgt cagcttcttg cgcacgccct tgatgcgcga catcagctcg
     6121 ccgccctggc gggcgtcgac catcgggatc agccggtagc ccacttccag gcccagcgcg
     6181 tcgtcggcgg aaacgtcgtc ccacgacagc tccttgggcg gctgcgccgg cgcgggctgc
     6241 ggctgtgcgg cggcctcggt ggtctgctgc gccttctgct tcatgaaata ggcggcagca
     6301 ccggtggccg cggccagcgc caggaacacc aggttgggca tgccgggaat gacgccgagc
     6361 aggcccagga tgccggcggt gatgaccagc acgcgcgggt ccttgaacac ctgccccatc
     6421 acctgctggc ccagttccga cgagcgcgac atgcgcgtga ccagcatggc caccgcggtg
     6481 gacagcagca gcgcggggat ctgcgccacc aggccgtcgc cgatggtcag cagcgtgtag
     6541 ttcttcagcg cggcggacag cggcaggccg tgttccagcg tgcctacggc aagaccgccg
     6601 ccgatgttga tgatgaggat caggatgccg gccatggcgt cgccgcggat gaacttcgac
     6661 gcgccgtcca tcgagccgta gaaatccgct tcctcgcgca cctcgttgcg ccgctcgcgc
     6721 gcatcctcgc gcgtgagcag gccggcgttg aggtcggcgt cgattgccat ctgcttgccg
     6781 ggcagcgcgt cgagcacgaa gcgcgccgac acttccgaaa cgcgctcggc gcccttggtc
     6841 accaccatga agttgatgat ggtgaggatg atgaagacga tgaagccgac gctgtaatgc
     6901 ccgccgatga cgaactcgcc gaaggcctcg atcacgtggc cggcggcgga tgagccttcg
     6961 tgtccgtgca gcagcaccac gcgcgccgag gcgacgttga gcgcaagtcg cagcagcgtg
     7021 accagcagca gcaccgacgg gaaagccgag aaatccagcg gccgcatcac atacaccacc
     7081 gccagcagga tgacgatgga cagcgcgatg ttgaaggtga acagcacgtc aagtgccagc
     7141 ggcgccagcg gcaccaccat catcgccagg atcaccagca gcagcagcgg cgcgcccagc
     7201 ccgccggcgc ccactgcgcg caggcgctgc cagggtgact cggctgtcat tgccgctgcc
     7261 atcagttcgc gcgctccatg cgcgcgcttt tgcaagaacc gttctcggcc cgctacagat
     7321 tttcgtgcca aaaaaccggc taccgcaggg cggtttttta tgttattaaa tattgcatat
     7381 ttttataatc ataagatatt ttataacaac tcaaaccggc gtgcggtggc aggctaggct
     7441 cgctggcggg gctggcgcgc tgcccttacg atggccgtga accggtcgca aaccggccca
     7501 cacgtcaccc ggggaaatca tgaaccgtct gtcacctggc ctgctgctgc tggctaccct
     7561 gcccgcattc gccgccgacc aggcgccgga actggcggtg cgctttccgc atctgtacgt
     7621 ggagtaccgg ctgggcgacg acggcgccgc ggtggagacg cgcgactggg ccatgaccgt
     7681 gctgcagcag cgcgccatcg agcaggccaa gcagtcgtcc atcacctaca gcaccagcat
     7741 ccagcacgcc gaagtgctgg cggcctggac agagaaagcc gatggccggc gcatcgatgc
     7801 gcccaagtcc aattttcagc ttgaggccaa cagcggcaac gaccgcaacg caccggtgtt
     7861 ttccgaccag accacgctca ccgtggtgtt tcccgaagtg gaagtcggcg acaccgtgca
     7921 cttcaactac cgcgtcagcg agagcgaacc gatgtttccc ggccagttct ccgacatgga
     7981 aatcttcagc cgcagcaccg cttacgacga cgtgcgggtg agcctggacg cgcctgcggc
     8041 gctctggctg cagacgcagg ccaaccagat gcaggcacag ccggcggtgg agaaggacgg
     8101 ccgccgcatc ctcgcatgga cttacgccaa cccgaatccg gcgcgcgaca agcgccgcga
     8161 ttacagcgtg ttcgatatcg agagcgcgcc cggcgtgttg ttctccacgt ttcacgacta
     8221 tgccgccatc gcccaggcgt atgcggcacg tgcgcggccc aaggcgctgc ccgacgagcg
     8281 ggtgcgcagg ctcgctgccg acatcgttgg cgggcgcacc gacgcgcgcg agcaggccca
     8341 cgcgctgtac gactgggtag ccaccaacat cacctatggc ggcaactgca tcggcaccgg
     8401 cgcggtggtg ccgcacgacc tgtcgttcgt gctcgacaac aagatgggtg attgcaagga
     8461 tcacgcgacg ctgctgcagg cgctgctggc cgcgcgcggc atcgaggcga cgcaggcgct
     8521 ggtcaatgcc ggcagcggct accggctgcc gaaggttccg gtggtgtcgt acgtcaacca
     8581 cgtgatcaac tacctgccgg ccttcggcat ctatgccgat tccacctcgc aggacacgcc
     8641 gttcggcctg ctgccgtttg ccgacgccga caagccggtg ctgctggtcg atggaaaaga
     8701 cacggtgggc cacacgccgg ccagtgccgc cggacgccgg caccaggaca tgaagagcgt
     8761 gctgaccata gccgccgacg gctcggtgtc gggcgaggtg gaaatccatc tgggcggcgc
     8821 gctggcggca gcggcgcgag ccggcatgcg tggatttccc aaggacgagg aagccgatct
     8881 ggtggagcac atgctgcagc ggcgcggcta tatcggcaaa ggcagcttcg agcacgatga
     8941 tcccaaggat ctgactgaca gctaccgtta ccgcatcagc ttccagatgc aggactacac
     9001 ccagctgccg ggcccggccg gcttcgtcat cgcgccggta atttccagtt cggcgccgct
     9061 gggagaattc gcctcgaccg cggtggccga cgacgtcgac tacgacacca cctgcagcgg
     9121 cgccagttcg gtggaggaat acagctacca gctgccggcg aacatgaagg tgctggccac
     9181 gcccgacgat gtcgaactgt ccaatggatt cctgcactac cgcgcgcact atcacctggc
     9241 gggccgcagg ctcgaggtgc gccgcgagat cgacgaccga acgccgggca acgtgtgcgg
     9301 cccggagatt tccgccgcct acaagaaatt cgccaagggc atgctgcgcg acctcaaggc
     9361 ccagg
//
LOCUS       DASZZC010000177         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_592762,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000177 DASZZC010000000
VERSION     DASZZC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592762"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VHE37_03870"
     CDS             <1..553
                     /locus_tag="VHE37_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVT34693.1"
                     /translation="NALADGAYAYAPRSVGDLARPYYPDGIDGQPSGPLSKSHADWSV
                     FSDGLQLDLVNSAILQHVTFVLGGGPDVAPGACTGLPATPAGLSRLADGIQIFAGGAP
                     IYRGSTLVGAIGVSGDGIDQDDMVSFLGLYNAGLALGGAIANAPAAMRADQLVPQGAR
                     LRYVQCPQSPFVNSGEQNVCAGK"
     gene            652..2739
                     /locus_tag="VHE37_03875"
     CDS             652..2739
                     /locus_tag="VHE37_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HVT34694.1"
                     /translation="MLLALALDARAQERSYGPVKRDESLTTVARRVRPEDTRLATMQT
                     LVALFRANPDAFDDGDINRLKPGSVLIVPDAKTIRAIPERETHGVFELRVSGPENTPP
                     HPAQASYKKSTDIQNDKQLITAQKAVRFDEKTLQRIPEGHAEAPLPPAPPPLPDPNRA
                     SGLAPVPPPQAWSPDDAAPIPDRWRLLDTLGITHPRWYDPYHSNVLKGDKPLDAAHHF
                     FVLSVISDSVYEARQLPTPVGPQATTSAGANDIFGGGTQTLFNQNLIVSLVYLEGDTT
                     FQPPDFELHATPVFNFNDTQVDEERALKIDPRAGNRRADGYLGVQELFVDYHLRNVSD
                     RYDFDSIRFGIQPISLDFRGFLLQDDQLALRLFGNRDNNRWQYNLGWMRRLEKNSNSG
                     LNAIDRRLRVDDTFFANLYRQDFPVMGFTSQGVIAANLNREEGHRFYDDNGFLERPAS
                     FGTEQPRRYHVTYLGYNGDGHFGRINLTTSLYAALGRESRGVFSASARDIAAGFGAAE
                     ISFDQDWVRWKLSLLWASGDHDPYDGTANGFDAIFENPIFAGADTSFYIRQAIPLIGG
                     GGVALSMRNGVLNDLRTSKEHGQSNFTNAGTQLAGVGADFDLMPQLRLSTNFNYLRFD
                     QVAVLELARNQGGISRNIGLDLSGALIWRPWFSQNIVARLSAARLLPGAGFRDLFPEG
                     QPYSLLGNLILTY"
     gene            3066..>5246
                     /locus_tag="VHE37_03880"
     CDS             3066..>5246
                     /locus_tag="VHE37_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34695.1"
                     /translation="MHVNPGVVLGCADCHGGDAHVQAPPGASRPERSYEEAMERAHVL
                     PRYAAAWPSSANPQASYTLLNKEAPEFIRFINPGDYRVAREACGACHLTIIEAAERSL
                     MSTAVMFLEGAAYNNGILPDKRGVLGEAYTREGAPAMLRSPAAPDDNAQRKGVIAQTW
                     PLPAWEVMPPADIFRVFERGGRNINTQFPETGLPDSTGSLQRLEEPGRPDLHQSSRGP
                     GTGSRIAVPVLNLTKTRLNDPYAWFLGTNEQPGDYRSSGCSACHVIYANDRDPKHSGP
                     YAAYGHDGTSITLDPTIAHNQSGHPLKHEFTRAIPTSQCMVCHMHQPNIFLNSMLGYT
                     MWDYESDAPAMWPREQKHPSDHDIREINERNPEEAAIRGNWSDPDFLRQVWDLNPTLK
                     DTQFADYHGHGWNFRAIFKRDRHGNLLDKDGHIVDDKDPDKFHKAVHLMSIHVEKGMQ
                     CVDCHFAQDAHGNGNLMGEVAQAVEIDCQDCHGTVDRYPALRTSGPAAPPGGSDLTRL
                     RTQDGRLRFEWVNGKLMQRSAVTPNLEWEIHTVKDSVTPGNAHYNAKAARAKLMSHDV
                     SAQNWSASVKPGDRAHGDDQMLCATCHSSWTTSCFGCHLPIQANWKSERHHYEGGETR
                     NFATYNPQVARDDFFMLGRHGEAKGYRIAPIRSSSALVLSSTNSNRERIYIQQPPVSA
                     GGFSSQAFAPHYPHTERTVETKQCEDCHLSKAGDNNAWMASLLGL"
BASE COUNT          946 a         1867 c         1638 g          795 t
ORIGIN      
        1 caatgcgctg gccgacggcg cctatgccta cgcgccgcgc tcggtcggcg acctggcgcg
       61 gccgtactac ccggacggca tcgacggcca gcccagcggc ccgctgtcga aatcccatgc
      121 ggactggagc gtgttctccg acggcctgca gctggacctg gtgaacagcg cgatcctgca
      181 gcacgtgact ttcgtgctcg gcggcgggcc cgacgtggcg ccgggcgcct gcaccggcct
      241 gccggcgaca cccgcgggct tgagccgcct ggccgatggc atccagatct ttgccggcgg
      301 cgcgccgatc taccgcggca gcactctggt gggcgcgatc ggcgtctccg gcgacggcat
      361 cgaccaggac gacatggtca gcttcctcgg cctgtacaac gccgggctcg cgctcggcgg
      421 cgcgatcgcc aatgcgccag cagcgatgcg cgccgaccag ctcgttccgc agggcgcgcg
      481 gctgcgctac gtgcaatgtc cgcaatcgcc cttcgtcaat tccggcgagc agaacgtatg
      541 cgcgggcaag tgaaatccaa tgcgctcgcc ggtttttgca ggagcgggcc ttgcccgcga
      601 tttgcgcccg catcgcgcgc aacgcgcgct cctacatttg cgcttgcgtg cgtgctgctc
      661 gcgcttgcgc tcgacgcgcg cgcgcaggaa cgcagctacg ggccggtgaa gcgcgacgag
      721 tcgctgacca ccgtcgcgcg ccgcgtgcgg ccggaggata cgcggctggc gacgatgcag
      781 acactggtcg cgctgttccg cgccaatcct gatgcgttcg acgatggcga catcaaccgc
      841 ctcaagcccg gcagcgtgct gatagtgccg gatgcgaaaa ccatccgcgc catacccgag
      901 cgcgagacgc atggcgtgtt cgaactgcgc gtttccggtc cagaaaacac tccaccgcac
      961 cccgcccagg cgagttataa aaaatctact gatattcaaa atgataaaca attaataaca
     1021 gcacaaaagg cagtgcggtt cgacgaaaaa acgctgcagc gcatccccga gggacacgcc
     1081 gaagcaccgc tgccgcccgc cccgccgccg ctgcccgacc ccaaccgcgc gagcggcctc
     1141 gcgccggtgc cgccgccgca ggcatggtcg ccggacgacg ccgcgccaat cccggaccgc
     1201 tggcgcctgc tcgacacact cggcatcacg catccgcgct ggtacgaccc gtaccattcc
     1261 aacgtgctca agggcgacaa gccgctcgat gcggcgcatc acttcttcgt gctgtccgtg
     1321 atctccgatt cggtctatga agcgcgccag ctgccgacgc cggtcggccc gcaggcgacg
     1381 acctcggccg gcgccaatga cattttcggc ggcggcacgc agacgctgtt caaccagaac
     1441 ctgatcgtgt cgctggtcta tctggaaggc gacaccacgt tccagccgcc ggacttcgag
     1501 ctgcatgcca cgccggtgtt caacttcaac gacacgcagg tggacgagga gcgcgcgctg
     1561 aagatcgacc cgcgcgcagg caaccgccgc gccgacggtt acctgggcgt gcaggaactg
     1621 ttcgtcgact accacctgcg caacgtctcc gaccgctacg acttcgactc gatccgcttc
     1681 ggcatccagc ccatctcgct cgatttccgc ggcttcctgc tgcaggacga ccagctggcg
     1741 ctgcgcctgt tcggcaaccg cgacaacaac cgctggcagt acaacctggg ctggatgcgg
     1801 cggctggaga aaaacagcaa cagcgggctc aacgccatcg accggcgcct gcgcgtggac
     1861 gacaccttct tcgccaatct ctatcgccag gatttcccgg tgatgggctt cacctcgcag
     1921 ggcgtgatcg ccgccaacct caaccgcgag gaggggcacc gcttctacga cgacaacggc
     1981 tttcttgagc ggccggcctc gttcggcacc gagcagccgc gccgctacca cgtgacgtac
     2041 ctcggctata acggcgacgg gcacttcggc cgcatcaacc tcacaacgtc gctctatgcc
     2101 gcgctgggcc gcgagagccg cggcgtgttt tccgcatcgg cgcgcgacat cgccgccggt
     2161 ttcggcgcgg cggagatctc cttcgaccag gactgggtgc gctggaagct gtcgctgctg
     2221 tgggccagcg gcgaccatga cccctacgac ggcacggcaa atggtttcga tgcgattttc
     2281 gagaacccga tctttgccgg cgccgacacc agcttctaca tccgccaggc gatcccgctg
     2341 atcggcggcg gcggcgtggc gctgtcgatg cgcaacggcg tgctcaacga ccttcgcacg
     2401 agcaaggaac acgggcagag caatttcacc aatgccggca cgcagctggc cggcgtcggc
     2461 gccgactttg acctgatgcc gcagctgcgc ctgtcgacca acttcaacta cctgcgtttc
     2521 gaccaggtgg cggtgctgga gctggcacgc aaccagggcg gcatctcgcg caacatcggg
     2581 ctggacctgt cgggcgcgct gatctggcgg ccgtggttct cgcagaacat cgtggcgcgg
     2641 ctgtcggcgg cacggctgct gccgggcgcg ggcttccgtg atttgttccc ggaaggacag
     2701 ccctactcgc tgctcggcaa cctgattctc acttactgat ggccatgctc gacggctact
     2761 gcaagttgtc aacggctccg tccctcaccc ctgcccctct cccggcggga ggggggaaag
     2821 tctttccttc tcccaccggg agaaggtggc gcgcagcgcc ggatgaggga cgggggctct
     2881 gcgcgcacat cgctgcaatt tcgctcctgg caatctccat ctgtgctcac gccagcgaag
     2941 gcgagaagcc ccgcgacatt cactacgaga ccgccccgcc cgcgcccgcc gcgcagaccg
     3001 ctgacgaggc gcaggcgaaa agcgcgggct gcctgagctg ccacacgcaa accgatcacg
     3061 ccagcatgca cgtcaatccc ggcgtggtgc tcggatgtgc ggactgccac gggggcgacg
     3121 cgcacgtgca ggcaccgccg ggtgcttcgc ggccggagcg cagctacgag gaagcgatgg
     3181 agcgtgcgca cgtgctgccg cgctacgccg cggcatggcc gtcgtcggcg aatccgcaag
     3241 ccagctacac gctgctgaac aaggaagcgc cggagttcat ccgcttcatc aacccgggcg
     3301 actatcgcgt ggcgcgcgaa gcctgcggcg cctgccacct gactatcatc gaggcggccg
     3361 agcgcagcct gatgagcacg gcggtgatgt ttctcgaggg cgccgcctac aacaacggga
     3421 tcctgccgga caagcgcggc gtgctcggcg aggcctacac gcgcgaaggt gcgccggcga
     3481 tgctgcgctc gcccgccgcg cccgacgaca acgcgcagcg caagggcgtg atcgcgcaga
     3541 cctggccgct gccggcgtgg gaggtgatgc cgccggccga catcttccgc gtgttcgagc
     3601 gtggcggccg caacatcaat acacaattcc cggagaccgg cctgcccgat tccaccggca
     3661 gcctgcagcg gctggaagag ccgggccgcc cggacctgca ccagtcgagc cgtgggcccg
     3721 gcaccggcag ccgcatcgcc gtgccggtgc tcaacctgac caagacgcgc ctcaacgatc
     3781 cctacgcgtg gttcctcggt accaacgagc agccgggcga ctaccgctcc tccggctgca
     3841 gcgcctgcca cgtgatctac gccaacgacc gcgacccgaa acattccggt ccgtacgcgg
     3901 cgtacgggca cgacggcacg tcgatcacgc tcgatccgac catcgcgcac aaccagagcg
     3961 gccatccgct caagcacgag ttcacccgcg ccatccccac cagccagtgc atggtgtgcc
     4021 acatgcacca gccgaacatc ttcctcaact ccatgctcgg ctacaccatg tgggattacg
     4081 aatcggacgc gccggcgatg tggccgcgcg agcagaagca tccgtccgac catgacatcc
     4141 gcgagatcaa cgagcgcaat cccgaggaag cggccatccg cggcaactgg agcgaccccg
     4201 atttcctgcg ccaggtgtgg gacctgaacc ccacgctgaa ggacacgcag ttcgccgact
     4261 accacggcca cggctggaac ttccgcgcca tcttcaagcg cgaccgccac ggcaatctgc
     4321 tcgacaagga tggccacatc gtcgacgaca aggatcccga caaattccac aaggccgtgc
     4381 acctgatgtc gatccacgtc gagaaaggca tgcagtgcgt ggactgccac ttcgcgcagg
     4441 acgcgcacgg caacggcaat ctcatgggcg aagtggcgca ggcggtggag atcgactgcc
     4501 aggattgcca tggcaccgtc gaccgctacc cggccctgcg cacctccggt cctgccgcgc
     4561 cgccgggcgg ttctgacctc acgcgcctgc gcacgcagga tggtcgcctg cgcttcgagt
     4621 gggtcaacgg aaaactgatg cagcgctcgg cggtgacgcc gaaccttgaa tgggaaatcc
     4681 acacggtgaa agacagcgtg accccaggca acgcgcacta caacgcgaag gccgcgcgcg
     4741 ccaagctgat gtcacatgac gtgagcgcgc aaaactggag cgcgtcggtc aaacccggag
     4801 accgcgcgca cggcgacgac cagatgctct gcgccacctg ccacagctcg tggaccacct
     4861 cgtgcttcgg ctgccatctg ccgatccagg ccaactggaa gagcgagcgg catcactacg
     4921 aaggcggcga gacgcgcaac ttcgccacct acaacccgca ggtggcgcgc gacgatttct
     4981 tcatgctcgg ccgtcacggc gaagccaagg gttatcgcat cgcgccgatc cgctccagct
     5041 cggcgctggt gctgtcgtcc accaactcca accgcgagcg catctacatc cagcagccgc
     5101 cggtgtccgc cggcggattt tcctcgcagg catttgcgcc gcattacccg cacaccgagc
     5161 gcacggtgga gaccaagcag tgcgaggact gtcacctgtc gaaggctggc gacaacaacg
     5221 cgtggatggc ctcgctgctc ggcctg
//
LOCUS       DASZZC010000178         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_915653,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000178 DASZZC010000000
VERSION     DASZZC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915653"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..277
                     /locus_tag="VHE37_03885"
     tRNA            193..277
                     /locus_tag="VHE37_03885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Leu,seq:cag)
     gene            complement(431..1723)
                     /locus_tag="VHE37_03890"
     CDS             complement(431..1723)
                     /locus_tag="VHE37_03890"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVT34696.1"
                     /translation="MGKTVVVIGAQWGDEGKGKIVDLLTDRVQAVVRFQGGHNAGHTL
                     VINGVKTALNLIPSGIMRPGVSCFIGNGVVLSLPDLIKEIEKVEATGASVRDRLRISE
                     AAPLVLPVHARLDQARERARGEAKIGTTGKGIGPAYEDKVARRAVRVGDLFQREQLAA
                     KLGELLSFHNFVLVNYFKEAPEDFQKTLDALLGYADVIRPMVADVTELLRLQLRRGDN
                     VLFEGAQGALLDVDHGTYPYVTSSNTTAGGASTGSGVGPRELDYVLGIVKAYATRVGS
                     GPFPTEQDNDIGQHIRDKGAEYGTVTKRPRRCGWFDAVAARRSIFNNSVSGLCVTKLD
                     VLDEMDVIRICTGYQQNGKPLEVPPIGAEGYAGIEPIYEELPGWKCNTFGIKRYQDLP
                     AAARKYLKRMEEVTDAEVAIISTGPDRDHTMVLHNPFD"
     gene            complement(1838..2845)
                     /locus_tag="VHE37_03895"
     CDS             complement(1838..2845)
                     /locus_tag="VHE37_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HVT34697.1"
                     /translation="MSNKWMLPDGVEEALPPSSWAMEALRRKLLDTYRKQQYELILPP
                     LLEHLDSLLTGAGSDLERQTFKLTDPASGRLLGLRADMTPQAARIAARRYADVKVVKL
                     CYLGTVLRAHPDSLGGARSPRQVGCEYFGEPGIAADLEILQLMLKTLALAGVRDAHLD
                     LGHVGIYRAIAARLGLDADQEAALFDILQRKSQPDLRDFAAARELKPRVAQSLAELMD
                     LNGDAAVLKRAREVLWQVGPEVKGALDDLERTVAELRKTLPKLPIHIDLAELRGYRYQ
                     TGMVFAAFVPGHGRELARGGRYDGVGREFGVARPATGFSADLNELLRLGSSSTTREKH
                     G"
     gene            complement(2842..3033)
                     /locus_tag="VHE37_03900"
     CDS             complement(2842..3033)
                     /locus_tag="VHE37_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HVT34698.1"
                     /translation="MDIEWQLLARALALTLVLEGLLPFMAPSRLRQTMLRMAQLDDRV
                     LRTLGLLSMITGIVVLQLL"
     gene            complement(3045..>3944)
                     /gene="hflC"
                     /locus_tag="VHE37_03905"
     CDS             complement(3045..>3944)
                     /gene="hflC"
                     /locus_tag="VHE37_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HVT34699.1"
                     /translation="RPGAAMRSHGVLWVGLLLGLGILGMNTFFVVDQRERAVLFQLGE
                     IRKTDYAPGLQWKVPFLQSVQKFDSRILTFDSQVENFLTVEKKNVEVEYFIKWRIDDV
                     TAYYRATGGQELVASDRLSATINRGLRDEFGSRTIQQAVGGEETSGAERDVIAEDLKK
                     KAADNVRELGVAIVDVRIKSINLPKEVSDSVYDRMRAERTRVAADLRARGAEEAERIR
                     ADADRAAQITIADAYRDSEKLRGEGDAKAAEIYARAYGQDAEFYSFYRTLNVYRDTFK
                     DKDDVLVLEPKGEFFKYFRGPGK"
BASE COUNT          626 a         1303 c         1272 g          743 t
ORIGIN      
        1 tgcggctggg gatcggctgg tcgaagttct gttcggccat gccgtagtcc gggtccagcg
       61 cgcgccagcc ggtgagttca ggatcagctt ttttgttttt cattgattga ctttgcttgg
      121 ggtcgccgcg aaaatgcgcg cccttcggat acgtgtcttg cctgaagccg tgcctgaagt
      181 tacagcagat gggcccatgt ggcggaattg gtagacgcac cagtttcagg tactggcggg
      241 taaaaccgtg ggggttcgag tccctccgtg ggcaccaaga acaaaacaca ccggcttatg
      301 ccggtgtttt tgtttttgcc ctgggggaac ggtagtttcc catattcccc acgcggcctg
      361 cggccacgtc gcccgcttaa ctaaagggga caccaagcgt tggcgcatcg agcgccaacc
      421 catggaggag ttaatcgaac ggattgtgca acaccatcgt atggtcgcga tccggcccgg
      481 tggagatgat cgccacctcg gcatcggtca cttcttccat gcgcttgaga tatttcctgg
      541 ccgcggccgg caggtcctgg tagcgcttga tgccgaaggt gttgcacttc caccccggca
      601 gttcctcgta gatcggctcg atgccggcat aaccttcggc gccgatcggc ggcacttcca
      661 gcggcttgcc gttctgctgg taaccggtgc agatgcggat cacgtccatt tcgtcgagca
      721 cgtcgagctt ggtcacgcac aggcccgaca ccgagttgtt gaagatcgag cggcgcgcgg
      781 ccaccgcgtc gaaccagccg cagcggcgcg gacgcttggt caccgtgccg tactccgcgc
      841 ccttgtcgcg gatgtgctgg ccgatgtcgt tgtcctgctc ggtgggaaac ggcccgctgc
      901 ccacgcgcgt ggcgtaggcc ttgacgatgc ccaggacgta atccagttct ctcggcccga
      961 cacccgagcc ggtggacgca ccgcccgcag ttgtattcga cgaggttacg tacgggtaag
     1021 tgccgtgatc gacgtcgagc agcgcaccct gcgcgccctc gaacagcacg ttgtcgccgc
     1081 ggcgcagctg caggcgcagc agctcggtga cgtcggccac catcggccgg atcacatcgg
     1141 cgtagccgag cagcgcatcg agggtcttct ggaagtcctc gggggcttcc ttgaagtaat
     1201 tcaccagcac gaagttgtgg aacgacagca gctcgccgag cttggccgcc agctgttcgc
     1261 gctggaacag atcgcccacg cgcaccgcgc ggcgcgccac cttgtcctcg taagccgggc
     1321 cgatgccctt gccggtggtg ccgatcttgg cttcgccgcg tgcgcgttcg cgcgcctggt
     1381 ccagccgcgc atgcactggc agcaccagcg gtgcggcctc ggagatgcgc aggcggtcgc
     1441 gcacggaagc gccggtggct tcgacctttt cgatttcctt gatcaggtcg ggcagcgaca
     1501 gcaccacgcc gttgccgatg aagcacgaca cgccgggccg catgatgccg gagggaatca
     1561 ggttgagcgc ggtcttcacg ccgttgatca ccagggtgtg gccggcgttg tggccaccct
     1621 ggaagcgcac caccgcctgc acgcggtcgg tgagcaggtc gacgattttt cccttgccct
     1681 cgtctcccca ttgagcccct atgacaacaa ctgtcttgcc catagtcttc aacctctggt
     1741 agtgccgagg cattgcgccg ggctcaatgg ggcaacgccc cgcattccat ttcgcgctcg
     1801 ccaaggcgag cgcggggcgc cgatcagagc ggctgttcta cccatgtttc tctcgcgtcg
     1861 tcgaggagga accgagcctc agtaattcgt tcaggtcggc ggaaaatccc gtcgccggcc
     1921 gggccacgcc gaattcgcgg cccacgccgt cgtagcgtcc gccgcgcgcc agttcgcggc
     1981 cgtgtcccgg cacgaacgcc gcgaacacca tgccggtctg gtagcggtag ccgcgcagtt
     2041 cggccaggtc gatgtggatc ggcagcttgg gcaaggtctt gcgcagttcg gccacggtgc
     2101 gctcgagatc gtcgagcgct cccttcactt ccgggcccac ctgccacagc acttcgcgcg
     2161 cgcgcttgag cacggcggcg tcgccattca ggtccatcaa ctcggccagc gactgcgcca
     2221 cgcgcggctt gagctcgcgt gccgcggcga aatcgcgcag gtccggctgc gacttgcgct
     2281 gcaggatgtc gaacagcgcc gcttcctggt cggcgtccag gcccagccgc gcggcgatgg
     2341 cgcggtagat gccgacatgg ccgaggtcca ggtgcgcgtc gcgcacaccg gccagtgcca
     2401 gcgtcttcag catcagctgc aggatttcca ggtccgccgc gatgcccggc tcgccgaaat
     2461 attcgcaacc cacctgccgc ggcgaacgcg cgccgcccag gctgtcggga tgcgcgcgca
     2521 gcacggtgcc caggtagcac agcttcacca ccttcacgtc ggcatagcgc cgagcggcga
     2581 tgcgcgcggc ctgcggcgtc atgtcggcgc gcaggccaag caggcggccg ctggccggat
     2641 cggtcagctt gaaggtctgc cgctccaggt cggaaccggc gccggtgagc agcgaatcca
     2701 ggtgctcgag cagcggcggc aggatcagct cgtattgctg cttgcggtag gtgtccagca
     2761 acttgcgccg cagcgcttcc atggcccacg aactgggcgg cagcgcctct tcgacgccgt
     2821 cgggcagcat ccacttgttg ctcatagcag ttgcagcacc acgatgccgg tgatcatgct
     2881 gagcagtccg agcgtgcgca gcacgcggtc atccagctgg gccatgcgca gcatggtctg
     2941 ccgcaagcgt gacggcgcca tgaacggcag cagtccttcc agcaccaggg tgagggcgag
     3001 cgcccgcgcc agcaattgcc actcgatgtc catgcggctg tttcttattt tccgggaccg
     3061 cggaagtact tgaagaactc gcccttgggc tcgagcacca gcacgtcgtc cttgtccttg
     3121 aaagtatcgc ggtagacgtt gagcgtgcgg tagaacgagt agaactccgc gtcctggccg
     3181 taggccctgg cgtagatttc cgctgccttg gcgtcgcctt cgccgcgcag cttttccgaa
     3241 tcgcgataag cgtcggcgat agtgatctgc gccgcgcggt cggcatcggc gcggatgcgc
     3301 tcggcttcct cggcgccgcg cgcccgcaga tcggcggcca cgcgcgtccg ctcggcgcgc
     3361 atgcggtcat acaccgagtc gctcacttcc ttgggcaggt tgatcgactt gatgcgcacg
     3421 tcgacgatgg ccacgcccag ttcgcgcacg ttgtcggccg ccttcttctt caggtcctcg
     3481 gcgatgacat cgcgttccgc tccgctggtt tcctcgccgc ccacggcctg ctggatggtg
     3541 cgcgagccga actcgtcgcg cagtccgcgg ttgatggtgg cggaaagccg gtcggaggcc
     3601 accagttcct gcccgccggt ggcgcggtag taggcggtga catcgtcgat gcgccacttg
     3661 atgaagtact ccacttcgac gtttttcttc tccaccgtca ggaaattctc gacctggctg
     3721 tcgaaggtca ggatgcggct gtcgaacttc tgcaccgatt gcaggaacgg caccttccac
     3781 tgcaggccag gcgcatagtc ggtcttgcgg atctcgccca gctggaacag caccgcgcgc
     3841 tcgcgctggt cgacgacgaa aaaggtgttc atgccgagga tgccaagacc cagcagcaga
     3901 cccacccaga ggacgccgtg cgatctcatg gccgcccccg gtcg
//
LOCUS       DASZZC010000179         9107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_466052,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000179 DASZZC010000000
VERSION     DASZZC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466052"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VHE37_03910"
     CDS             complement(<1..302)
                     /locus_tag="VHE37_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34700.1"
                     /translation="MTSAGGGRPRLRGLILVNTAGPTARDDSAMNLPIAWITHPDCLR
                     HDMGEEHPECPARLRAIEERLHEAGIWDFLAHHAAPEAPRAALQRVHDAAYVQRML"
     gene            398..1168
                     /locus_tag="VHE37_03915"
     CDS             398..1168
                     /locus_tag="VHE37_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVT34701.1"
                     /translation="MAFDTRPVSSEIRSCSRVAANCAACPSSRHCLVGGAAQNETARW
                     NAAVQIQQSIPAGKSLYAVGDEVDAVYTVRAGCVKTYTVDDEGNERVRAFHLPGDIVG
                     LDALGSGRHLSHAVAVTPAQICRVPREQMRKLLVESPTLMLRLVDRMSATLAAALALA
                     GDYTADQRVAAFLVSMHQKLDPLPGAPARLPMCRRDIANFLRLATETVCRVLTRFEKQ
                     GLISTDDRAFRILRQDALRELAEPVGITAPAAPVRLAA"
     gene            complement(1173..2315)
                     /locus_tag="VHE37_03920"
     CDS             complement(1173..2315)
                     /locus_tag="VHE37_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34702.1"
                     /translation="MKFYSQGGQDQYLLESFFRGRRGGSFVEIGAAPDAGNTLMFERH
                     MDWRGLRAAGGIELADLLRQQAMTGFDYVSIGPQAPADTVAALDAALAGARVISVSRT
                     AAAAAPAAQWLGAHGYQLHARLQHDDIYRRPETCAQARTSVICAVWHGDVQRHELLRG
                     HMDNLARQSVPVDPIYVFDGGGSPPAWLAGQVVSVRQPLTIYQAWNVALSLVATPMVM
                     NLNLDDRLAPDAVALLERELIRNRAAVIGADWKLCYTQAETDAVQPCMPAAQMPLVWG
                     WPPQPGTLTRLGSGRERDTLGPATLWRMEAHVGLPRYPWRLRDGSLLRSSADYGWWKL
                     LQANPKFRLVSLPTIIGNYHSHPGEQAEFRYGDERRLMADPGIAPY"
     gene            2386..3231
                     /locus_tag="VHE37_03925"
     CDS             2386..3231
                     /locus_tag="VHE37_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT34703.1"
                     /translation="MPACHGMIAALLGVVVGAVLGLTGAGGSVLAVPLLMAGLGWSLP
                     QAAPVALLAVAAAAAMGAWTAWRKSYLRYRAALLMAMLGWLTAPLGVHAAAALPAPLL
                     MGLFAAVMLLVALRLWRQARVAPDEARVVRADVHGEGAASRGPLLRLDPQTGRIDWDW
                     SAALVIGAIGALTGLVSGLLGVGGGFVIVPALRAASPLSMNSAVATSLMVIALVAGGA
                     VLASVWQGHALPGAALPFAAGSIAGMMAGRALAERLAGPRLQRGFALLLLLVGLGMAA
                     RAAGV"
     gene            3244..3936
                     /locus_tag="VHE37_03930"
     CDS             3244..3936
                     /locus_tag="VHE37_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT34704.1"
                     /translation="MLLRQLFDRESSTYTYLLAGDAGEALLIDPVQAQLDSYLQLLRE
                     LKLSLVLALDTHTHADHVTAMGDLRSATGCVTMMGRQSQAECVSARFDDEQVVGCNGV
                     ELRALYTPGHTDESYSFFTRSGGVPMVFTGDALLIRGTGRTDFQNGDARAAYRSLFNR
                     LLTLPDETLVYPGHDYKGWSVSTIGEERLHNPRLQVKSADEYAALMASLKLPSPALMD
                     VALPANLRCGRR"
     gene            3981..4508
                     /locus_tag="VHE37_03935"
     CDS             3981..4508
                     /locus_tag="VHE37_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVT34705.1"
                     /translation="MDIRVTTPERDLNLLLSRLQDVGLFENLARDQLVTLLGLAQKMT
                     FAQGELLFAEGDDGDCMHLLVSGMVEICKTAADGQLRSFAIVQPGEPVGEMALIERGP
                     RAASVRALEPCVTLQLSRAALTRQPRIEATVYRNLARVLVRRLRQADQDLIAITAAGD
                     APPAAVPAGSAVTTQ"
     gene            4598..7456
                     /locus_tag="VHE37_03940"
     CDS             4598..7456
                     /locus_tag="VHE37_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT34706.1"
                     /translation="MDRRLQLAARAADVQVWEWTVATGELWWNDAAAEQLDLEPGTKP
                     SYEAWLACVHPDDRELARRMVDEAVADPGRHDCNFEFRLRRRNGEIRWMRTRAVIVRD
                     SAGTAVSMIGADVDITDLRALIAARDEQATLAWSLFDQSPIGAMVLGLDGCIERSNAS
                     FQQLLGYNAAQLQGMSVETLTAPGDLAMTRQRMRELGQGRGEPTGYEKRYLARDGRPV
                     PVRVHTACLHVGGKPWKLIGLVTDLSTERASRQQLHEMSERLQLATAGQGVGVWDLDI
                     GDWSLRLDEASAALFDLDARERVSFSQWLARLHVDDQAELQATVRRLLESNEGLFEME
                     FRVLRADGALRWVHSSGRIVRDAAGKALRAVGLNRDVSTVHRQLEEMQLQATAMNAAS
                     NAMIITDRNGHIVWVNQAFSRITGYRADEAIGQTPRLFKSGHQSAEWYRSMWATISAG
                     QVWRGRIVNRAKDGTLLDIEQTITPVRDVHGKIGHFVSVHEDVTEQVRSEQRIHQLAM
                     VDGVTGLPNRNAFQQHVTNALARAQRSGRPLAVMLLDLDHFKHVNDTLGHAAGDQLLA
                     EVGQRLRAQLREGDFAARLGGDEFALLAEDVTQPKQAMLTAERVLKSVREPYQLAGHA
                     AHVGASIGITLSQDGATGELLLKQADLAMYQAKTRGRDRFRFFDGEMDAEARRRFELE
                     SALRAALQNDRLTLMYQPQFDLRSGQLTGMEALLRWTDPEHGAVSPAEFVPLAEQCGL
                     GVELGRWVLHHALLQAGEWKRLGRHVPVAVNTNAQQFEQADFYYQVESGLRACGLDSD
                     DIEIEITESVMLQNSRIVQQNVQCFREAGIKLAVDDFGTGYSSLVSLRDYPVSRLKID
                     QSFVRGIGGDGKDERIVRAVLALARSLDLHVIAEGVETESQERFLRGEGCQEVQGFRY
                     ARPLSAEAMLSLLETGRAAPYVGPALPGAPAASPPAAH"
     gene            7507..7680
                     /locus_tag="VHE37_03945"
                     /pseudo
     CDS             7507..7680
                     /locus_tag="VHE37_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
     gene            7773..8186
                     /locus_tag="VHE37_03950"
                     /pseudo
     CDS             7773..8186
                     /locus_tag="VHE37_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
     gene            8256..>9107
                     /locus_tag="VHE37_03955"
     CDS             8256..>9107
                     /locus_tag="VHE37_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT34707.1"
                     /translation="MARLAAIVESSSDAILGKDLDGNITSWNKAAEQIYGYQAHEVLG
                     KPVSMLFPPDRLREEDGILDKIRHGEQIQHLETERVTKEGRRIAMSLTISPIFDAQGR
                     LLGASTIGRDISERRRADVRFRSLLETAPDAMIIIGRDGRIALVNAQSEKLFGYTRDE
                     LIGQSVEALIPERLRNKHAAHRTGYFQAPKVRGMGAGLELSGRRKDGSEFPIEISLSP
                     MESGEQLYATAAVRDITERKLVEQKLAAYAEGLERSNEELAQFAYVASHDLRAPLRSV
                     SGFAQLLQ"
BASE COUNT         1372 a         3099 c         3246 g         1390 t
ORIGIN      
        1 tccagcatgc gctgcacgta ggcggcatcg tgcacgcgct gcagcgcggc gcgcggcgcc
       61 tcgggcgccg cgtggtgcgc caggaaatcc cagatgccgg cctcgtgcag ccgctcctcg
      121 atggcgcgca gccgcgcggg acattcggga tgttcctcgc ccatgtcgtg gcgcaggcag
      181 tcggggtggg tgatccaggc gatcggcagg ttcatggcgc tatcatcgcg cgcagtcggc
      241 ccggcggtat tgaccaggat caagccgcgc agccgcggcc gtccgccgcc cgccgaagtc
      301 aactacgtag ttgccgcaga ggccctgcgg gatttgcgaa ttttgcgtag ccgcggcaaa
      361 gcggatgctg gcgccacttc aaaacgaggt gcgtgccatg gctttcgata cccgtccggt
      421 ttcctccgaa attcgcagct gcagccgcgt ggccgccaac tgcgccgcct gcccgtccag
      481 ccgccactgc ctggtcggcg gcgctgcgca gaacgagacg gcgcgctgga atgccgcggt
      541 gcagatccag caatcgatcc ccgccggcaa gtcgctgtat gcggtgggcg acgaagtcga
      601 cgccgtgtac accgtgcgcg ccggctgcgt gaagacctac acggtggacg acgagggcaa
      661 cgagcgggtg cgcgcgttcc acctgccggg cgacatcgtc ggcctggatg cgctgggttc
      721 gggcaggcac ctgtcgcatg ctgttgcggt gacgccggcg cagatctgcc gcgtgccgcg
      781 cgagcagatg cgcaagctgc tggtcgagtc gccgacgctg atgctgcggc tggtggaccg
      841 catgagtgcg acgctggccg cggcgctggc actggcgggc gattacaccg ccgaccagcg
      901 cgtggccgcc ttcctggtca gcatgcacca gaagcttgat ccgctgcccg gcgcgcccgc
      961 gcgcctgccg atgtgccgcc gcgacatcgc caacttcctg cgcctggcca ccgagacggt
     1021 gtgccgcgtg ctgacgcgct tcgagaaaca ggggctgatc agcaccgacg accgcgcctt
     1081 ccgcatcctc aggcaggacg cgctgcgcga actggccgag ccggtgggca tcaccgcgcc
     1141 ggcggcgccg gtgcgcctgg cggcgtgact gcctagtacg gcgcgatgcc gggatcggcc
     1201 atcagcctgc gctcgtcgcc gtagcggaat tccgcctgct cgccgggatg gctgtggtag
     1261 ttgccgatga tggtgggcag cgagaccagc ctgaacttcg gattggcctg cagcagtttc
     1321 caccagccgt agtcggcgct gctacgcagc agcgaaccgt cgcgcagccg ccacgggtag
     1381 cgcggtagcc ccacgtgcgc ttccatgcgc cacagcgtcg caggtcccag cgtgtcgcgt
     1441 tcgcgcccgc tgcccaggcg ggtcagcgtg cccggctgcg gcggccagcc ccataccagc
     1501 ggcatctgcg cagcgggcat gcacggctgc actgcgtcgg tttccgcctg cgtgtagcac
     1561 agcttccagt cggcgccaat cacggcggcc cggttgcgga tcagttcgcg ttcgagcagc
     1621 gcgacggcgt cgggcgcgag gcggtcgtcg aggttgaggt tcatcaccat cggcgtggcc
     1681 accagcgaca gcgccacgtt ccatgcctgg tagatggtca gcggctggcg cacgctgact
     1741 acctggccgg ccagccaggc gggcgggctg ccgccgccgt cgaacacgta aatgggatcc
     1801 accggcacac tctgccgcgc caggttgtcc atgtgcccgc gcagcaattc atgccgctgt
     1861 acatcgccat gccataccgc gcagatcacc gaggtacgcg cctgcgcgca cgtctccggc
     1921 cgccggtaga tgtcgtcgtg ctgcaggcgc gcgtgcagct ggtagccgtg cgcgccaagc
     1981 cattgcgccg ccggcgccgc tgccgcagcc gtacgcgaca cgctgatgac gcgtgcgccg
     2041 gcgagggcgg catcgagcgc ggcgacggtg tctgcgggcg cctgcgggcc gatcgagaca
     2101 tagtcgaagc cggtcatggc ctgctggcgc agcaggtccg cgagttcgat gccgcctgcc
     2161 gcgcgcagtc cgcgccagtc catgtgccgc tcgaacatca gcgtgttgcc ggcatccggc
     2221 gccgcgccga tttccacgaa gctgccgccg cgccggccgc ggaagaagct ttcgagcagg
     2281 tactggtcct gaccgccctg cgaatagaat ttcaagcgtc ttcccttggt cggtgccgcg
     2341 cctggcggag ttcctcgtga gccaactttt tagcacagcg acgatgtgcc cgcgtgccac
     2401 ggcatgatcg cggcgctgct cggcgtggtg gtgggtgcgg tcctcgggct taccggtgcg
     2461 ggcggctcgg tgctggccgt gccgctgctg atggcgggcc tgggctggag cctgccgcag
     2521 gccgcgccgg tggcgctgct ggcagtggcg gcggccgctg ccatgggcgc atggacagcc
     2581 tggaggaaaa gctacctgcg ttaccgggcg gcgctgctca tggccatgct gggttggctc
     2641 accgcgccgc tgggcgtgca cgctgccgcg gcgctgccgg cgccgctgct gatgggcctg
     2701 ttcgccgcgg tcatgctgct ggtggccctg cgcctgtggc gccaggcgcg cgtggcaccg
     2761 gacgaggcgc gcgtggtgcg cgccgacgtg catggcgaag gcgcggcctc gcgcggtccg
     2821 ctgctccggc ttgacccgca gaccggccgc atcgactggg actggtcagc agcactggtc
     2881 atcggcgcca tcggcgcgct gaccgggctg gtctccggcc tgctgggcgt gggcggcggc
     2941 ttcgtcatcg tgccggcgct gcgcgcggcc agcccgctgt cgatgaactc ggcggtggcc
     3001 acctcgctga tggtgatcgc gctggtggcg ggcggagcgg tgctggcctc ggtgtggcag
     3061 ggccatgcgc tgccgggggc cgcgctgcct ttcgccgcgg gcagcatcgc cggcatgatg
     3121 gcgggacgcg cgctggccga acgcctcgcc gggccgcgcc tgcagcgcgg cttcgccctg
     3181 ctgctgctgc tggtggggct gggcatggcg gcgcgcgccg ccggtgtttg acggagagcc
     3241 gacatgctgc tgcgccagtt gttcgaccgc gaatccagca cttacaccta cctgctcgcc
     3301 ggcgatgctg gcgaggcgct gttgatcgac ccggtgcagg cgcagctcga cagctacctg
     3361 cagctgctgc gcgaactgaa actgagcctg gtgctcgcgc tcgacacaca cacgcatgcc
     3421 gatcacgtca ccgccatggg tgacctgcgc agcgctaccg gctgcgtgac catgatgggc
     3481 cggcagagcc aggccgaatg cgtgagcgcg cgctttgacg atgagcaggt cgttggctgc
     3541 aacggcgttg aactgcgcgc gctgtacaca cccggccaca ccgacgagtc ctacagcttc
     3601 ttcacgcgca gtggcggcgt gccgatggtg ttcaccggcg atgcgctgct gatccgcggc
     3661 accggccgca ccgatttcca gaacggcgat gcgcgtgcgg catatcgctc gctgttcaac
     3721 cgcctgctca cgctgcccga tgaaacgctg gtgtatcccg gccacgacta caagggctgg
     3781 agcgtgtcca ccatcggcga ggagcgtctc cataacccgc ggctgcaggt gaaatccgct
     3841 gacgaatacg cggcgctgat ggcgtcgctc aagctgccca gtcccgcgct gatggacgtg
     3901 gcgctgccgg ccaacctgcg ctgcggacgc cgctgatcca ggtcaatgcc gtccggccgc
     3961 cgttgccgga gactggttgc atggatatcc gcgtcaccac acccgagcgt gacctcaacc
     4021 tgctgctgtc gcgcctgcag gacgtgggcc tgttcgagaa tctggcgcgc gaccagctgg
     4081 tgacactgct ggggctggcg cagaagatga cctttgccca gggcgaactg ctgtttgccg
     4141 aaggcgacga cggcgactgc atgcacctgc tggtcagcgg catggtcgag atctgcaaga
     4201 ccgcggccga cggccagctg cgcagctttg ccatcgtgca gccgggcgag ccggtcggcg
     4261 agatggcgct gatcgagcgc ggcccacgcg cggccagcgt gcgcgcgctg gaaccctgcg
     4321 tgaccctgca gctgtcgcgc gcggcgctga cgcggcagcc gcgcatcgag gccaccgtgt
     4381 accgcaacct ggcgcgcgtg ctggtgcggc ggctgcggca ggccgaccag gaccttatcg
     4441 ccatcactgc agcgggcgat gcaccgccag ccgccgtgcc cgccggctcc gctgtcacga
     4501 cgcaatgagc ggccgggcgg gggaaggcgg aacgccggca cccgctgccg gcgaggcggt
     4561 gccatcgact ttgtcgtgct gcccgcagtc cgaacggatg gaccgccgcc tgcagctggc
     4621 cgcgcgggcg gccgatgtgc aggtgtggga atggacggtt gccaccggcg agctgtggtg
     4681 gaacgacgcc gccgcggagc agctcgacct ggaacccggc accaagcctt cgtacgaagc
     4741 ctggctggcc tgcgtgcacc ctgatgaccg cgagctggca cggcgcatgg tcgacgaagc
     4801 cgtcgccgac ccgggtcggc acgactgcaa cttcgagttc cgcctgcgcc ggcgcaacgg
     4861 tgaaatccgc tggatgcgta cgcgcgccgt catcgtgcgc gacagcgccg gtaccgcggt
     4921 cagcatgatc ggcgccgacg tcgacatcac cgacctgcgt gcgctgatcg ccgcgcgcga
     4981 cgagcaggca accctggcct ggtcgctgtt cgaccagtcg cccatcggcg ccatggttct
     5041 cggactggat ggctgcatcg agcgcagcaa cgcaagcttc cagcagctgc tgggctacaa
     5101 cgcggcgcag ctgcagggca tgtcggtgga aaccctcacc gctccgggcg acctcgccat
     5161 gacgcgccag cgcatgaggg aactgggcca ggggcgcggt gagcccacgg gctacgagaa
     5221 gcgctacctg gcgcgcgacg gccggccggt tccggtgcgc gtgcataccg cctgcctgca
     5281 tgtcggcggc aagccgtgga agctgattgg cctggtcacc gatctgtcga ccgagcgcgc
     5341 ttcgcgtcag caactgcacg agatgtccga gcggttgcag ctggccacgg ccggccaggg
     5401 tgtgggcgtg tgggacctgg acatcggcga ctggagcctg cggctcgacg aggccagtgc
     5461 cgcgctgttc gatctcgatg cgcgggagcg ggtgagtttt tcccagtggc tggcgcggct
     5521 gcacgtcgac gaccaggccg agctgcaggc gaccgtgcgc aggctgctcg agtcgaacga
     5581 gggcctgttc gagatggaat tccgcgtgct gcgtgccgac ggcgcgctgc gctgggtgca
     5641 cagcagcggc cgcatcgtgc gcgacgctgc cggcaaggcg ctgcgcgccg tcggactgaa
     5701 ccgcgacgtc agcaccgtgc accgccagct ggaggagatg cagctgcagg ccacggcgat
     5761 gaatgcggca tcgaacgcca tgatcatcac cgaccgtaac ggccacatcg tctgggtcaa
     5821 ccaggcgttc agccgcatca ccggctaccg cgcggacgag gccatcggcc agaccccgcg
     5881 cctgttcaag tccgggcacc agtcggccga gtggtaccgc agcatgtggg ccaccatcag
     5941 tgccggccag gtgtggcgcg gccgcatcgt gaaccgggcc aaggacggca ccctgctcga
     6001 catcgagcag accatcacgc cggtgcgcga cgtgcacggc aagatcggcc attttgtctc
     6061 ggtgcacgag gacgtgaccg agcaggtgcg ctcggagcag cgcatccacc agctggcgat
     6121 ggtcgatggc gtcaccggcc tgcccaaccg caacgctttc cagcagcacg tgaccaacgc
     6181 gctggcgcgc gcgcagcgca gcggccgtcc gctggcggtg atgctgctcg acctggatca
     6241 cttcaagcac gtcaacgaca ctctgggcca tgccgccggc gaccagttgc tggcggaagt
     6301 cggccagcgc ctgcgcgcgc aactgcgcga gggcgacttc gccgcgcgcc tgggcggcga
     6361 tgaattcgcc ctgctggccg aggacgtgac ccagcccaag caggccatgc tcaccgccga
     6421 acgcgtgctt aagtcggtgc gcgagccgta ccagctcgcc ggccatgcag cgcatgtggg
     6481 cgccagcatc ggcatcacgc tgtcgcagga cggtgccacc ggcgagctgc tgctcaagca
     6541 ggcggacctg gccatgtacc aggccaagac acgcggccgc gaccgcttcc gcttcttcga
     6601 tggcgagatg gacgccgagg cgcggcgccg cttcgagctc gaatcggcat tgcgcgcggc
     6661 gctgcagaac gatcggctca cgctgatgta ccagccgcag ttcgacctgc gcagcgggca
     6721 gctgaccggc atggaggcac tgctgcgctg gaccgatccc gaacacggcg cggtttcacc
     6781 ggccgagttc gtgccgctgg ccgagcagtg cggcctgggc gtcgaactgg gccgctgggt
     6841 gctgcaccat gcgctgctgc aggccggcga gtggaaacgg ctgggccgcc acgtgccggt
     6901 ggcggtgaac accaacgcac agcagttcga gcaggccgat ttctattacc aggtggaatc
     6961 cgggctgcgc gcctgcgggc tggacagcga cgacatcgag atcgagatca ccgagagcgt
     7021 gatgctgcag aacagccgca tcgtgcagca gaacgtgcag tgcttccgcg aggccgggat
     7081 caagctggcg gtggacgatt tcggcaccgg ctactcctcg ctggtgtcgc tgcgcgatta
     7141 cccggtgagc cgcctgaaga tcgaccagtc gttcgtccgc ggcatcggcg gcgacggcaa
     7201 ggacgagcgc atcgtccgcg cggtgctggc gctggcgcgc tcgctggacc tgcacgtgat
     7261 cgccgaaggc gtggagaccg agtcgcagga gcgcttcctg cgcggcgagg gctgccagga
     7321 ggtgcagggc ttccgttacg cgcggccgct gagcgccgag gcgatgctgt cgctgctcga
     7381 gactggccgc gcggcaccgt atgtcggccc ggcgcttccg ggtgcgccgg cggcttctcc
     7441 accggccgcg cattagcggc tcaataaggc cacatggacg acatcttcca ccgggggacg
     7501 gctgtcatgg ctgaagtgga ccctgcgtgg taccgcgaac tggtcgaatc ggcgcccgat
     7561 gccacggtgc tgatcgacag tgccggcttc atcatgctgg tcaatgcgca ggccgagcgg
     7621 ctgttcggct acggccgcgc cgaactgctc ggccagccgg tcgagatgct ggtgcccgag
     7681 cgcttccgcc gcggccatgg cgtgcaccgc gacggctact tcgccgagcc caaggtgcgc
     7741 gacatgggcg ccggcgtgga actggccggg cggcgcagga cggcagcgag ttcccggtgg
     7801 agatcagcct gtcgccgctg cgcaccgcgc gcggcgtgtt cgccaccgcg gcgatccgcg
     7861 ataccaccga ccgcaagaag gcgcaggacc agttccgcgc gctgctggaa accgcgccgg
     7921 atgccatggt catcatcgac gacagtggcg tgatccggct ggtcaacgcg caggccgagc
     7981 gcctgttcgg ctacgcgcgc gaatcgctgg tgggcaaacc ggtggaggtg ctgattcccg
     8041 agcgcctgcg cggcaagcat gccggccacc gctcgggtta ttttcatgcg ccgaaggtgc
     8101 gcggcatggg tgccgggctg gaactgtggg gccggcgcaa ggacggcatc gagtttccca
     8161 tcgagatctc gctgtcgccg ctggagaccg ccggcggcac ctgggccacc gccgcggtgc
     8221 gcgacatcac cgaccgcaag cacatcgcca gcaccatggc gcggctggcg gccatcgtcg
     8281 aatcgtccag cgatgccatc ctgggcaagg acctggacgg caacatcacc agctggaaca
     8341 aggccgcgga acaaatctac ggctaccagg cgcacgaagt gctgggcaag ccggtgtcga
     8401 tgctgtttcc gcccgaccgc ctcagggagg aagacggcat cctcgacaag atccgccacg
     8461 gcgagcagat ccagcacctg gaaaccgagc gcgtgaccaa ggaagggcgg cgcatcgcca
     8521 tgtctctgac catctcgccg attttcgatg cgcagggccg cctgctcggc gcctccacca
     8581 tcggccgcga tatctccgag cggcgccgcg ccgacgtgcg cttccgctcg ctgctggaaa
     8641 ccgcgccgga cgccatgatc atcatcggcc gcgacggccg catcgcgctg gtcaacgcgc
     8701 agagcgagaa gctgttcggc tacacccgcg acgagctgat cggccagtcg gtggaagcgc
     8761 tgatcccgga gcgcctgcgc aacaagcatg cggcgcaccg caccggttat tttcaggcac
     8821 ccaaggtgcg cggcatgggc gccgggctgg agctgtcggg gcggcgcaag gacggcagcg
     8881 agtttcccat cgagatctcg ctgtcgccga tggaaagcgg cgagcagctg tatgccaccg
     8941 cggcggtgcg cgacatcacc gagcgcaagc tggtggaaca gaagctggcg gcttacgccg
     9001 aagggctgga gcgcagcaac gaggagctgg cgcagttcgc ctacgttgcc tcgcacgacc
     9061 tgcgtgcgcc gctgcgcagc gtgagcggct ttgcccagct gctgcag
//
LOCUS       DASZZC010000180         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_723531,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000180 DASZZC010000000
VERSION     DASZZC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723531"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="VHE37_03960"
     CDS             complement(<1..1729)
                     /locus_tag="VHE37_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVT34708.1"
                     /translation="MTLHAALNHVTRYRYDRPVSLSPQLIRLRPAPHARTPVIGYSLK
                     VEPEKHFINWQQDPHGNWLARVVFPDQVTEFCVTVDLTADMAVYNPFDFFVEPAAEIF
                     PFEYEAALKEDLKPYMRAAPVGPLLKKYLAALPRREKVKSVDFLVDLNRALQQQTRYL
                     IRMEPGVQTPEETLEKASGSCRDTSWLLVQILRQLGLAARFVSGYLIQLKPDVKSLDG
                     PSGTDHDFTDLHAWAEVFLPGAGWIGLDPTSGLLAGEGHIPLCATPEPQSAAPISGLV
                     SESKVEFEHEMKVTRLFESARVTLPYSDAQWRAVDALGDLVDARLKANDVRLTMGGEP
                     TFISLDDMQSAQWNTAAVGPEKQKLAWELTQRLRERFAPHGLLTYGQGKWYPGESLPR
                     WVYSLYWRRDAKPIWRLPPASPRTLDPPTVKDARVFIRELAQRLELDKGCVLEAHEDA
                     LHYMVKERRLPINVDAADNKLKDPEERQRMLDVFERGLGLARGYVLPVQRAQAQGRWQ
                     SERWQLRGGKLFLLPGDSPIGLRLPLDSLPWLPAKERPEVIAADPGALPQEFPAADPR
                     RQPYLQVQQI"
     gene            1815..2699
                     /locus_tag="VHE37_03965"
     CDS             1815..2699
                     /locus_tag="VHE37_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34709.1"
                     /translation="MKSSAQPTLDSTSDFIPIRNLKYHVRRWGARDKPMLFLTHGWLD
                     VSETMHLMVQPLLARFQVLAPDWRGLGLTEWPQDGYWFPDYVGDLEAVIDHYSPAQPV
                     LLAGHSMGAQAASLYAGLRATRVAKLILMDALFLPDMPAELAPRRYRDWLEDLKDLPQ
                     PLHYASFEELAQRVKKKHPQLSDERALFVARCWGRARDDGRVHLLADPKHRLNGPGLF
                     RVAESIEIWKQVAAPTLFLDAGKSIFRQALSKEELERRRSCFRGRHEQVIEQAGHMLH
                     FDEPEATGRAMLEFLVNG"
     gene            2696..3388
                     /locus_tag="VHE37_03970"
     CDS             2696..3388
                     /locus_tag="VHE37_03970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT34710.1"
                     /translation="MSTPNPAAPKPAHPHCLIVGVGPGIGQALALAFARENYNVSLLA
                     RNPEKLADTVAMVRQKTARDARAFRADAGVEASLKAAMVHARRAFGELDVLIFNAAHA
                     ERGKPTTLSVEQLVSNFTTNVGGALVAAREAANDMRRKKAGSILFTGGGFAHEPAAEY
                     ASLSLDKAALRSLTYTLAQELGGDGIHVATVTVYGFVQTGTRFDPSRIAQQFVALHRQ
                     PKGHFDTEVVFK"
BASE COUNT          593 a         1236 c         1214 g          588 t
ORIGIN      
        1 tgatctgctg cacctgcagg tacggctggc gccgcggatc ggcggcggga aattcctgcg
       61 gcagcgcgcc ggggtcggcc gcgatcacct cgggccgctc ctttgccggc agccacggca
      121 gggagtccag cggcaggcgc aggccgatcg gcgagtcgcc cggcagcagg aacaattttc
      181 cgccgcgcag ctgccagcgc tcggactgcc agcggccctg cgcctgcgcg cgctgcaccg
      241 gcagcacgta gccgcgcgcc aggcccaggc cgcgctcgaa cacgtcgagc atgcgctggc
      301 gttcctccgg gtccttcagc ttgttgtcgg cagcgtcgac gttgatcggc aggcgccgct
      361 ccttgaccat gtaatgcagc gcgtcctcgt gggcctcgag cacgcagccc ttgtccagct
      421 ccagccgctg cgccagctcg cggatgaata cgcgcgcgtc ctttaccgtg ggcgggtcga
      481 gcgtgcgcgg cgaggccggc ggcaggcgcc agatgggttt cgcgtcgcgg cgccagtaca
      541 gcgagtagac ccagcgcggc agcgactcgc ccgggtacca cttgccctgg ccgtaggtga
      601 gcaggccgtg cggggcgaag cgctcgcgca ggcgctgggt cagttcccag gccagcttct
      661 gtttctccgg gccgaccgcg gcggtgttcc attgcgccga ctgcatgtcg tcgagcgaaa
      721 tgaaggtcgg ctcgccgccc atggtcaggc gcacgtcgtt ggccttcagc cgcgcatcga
      781 ccaggtcgcc caatgcatcg accgcgcgcc actgcgcgtc cgagtacggc agggtcacgc
      841 gcgccgattc gaacaggcgc gtgaccttca tctcgtgctc aaactcgacc ttgctctcgc
      901 tgaccagccc gctgatcggc gcggcggatt gcggctcggg cgtggcgcac agcgggatat
      961 gcccttcgcc ggcgagcagg ccggaggtgg gatcgagccc gatccagccg gcgccgggca
     1021 ggaacacctc ggcccaggca tgcaggtcgg tgaagtcgtg atcggtgccg gaaggcccgt
     1081 cgagcgactt cacgtccggc ttcaactgga tgagatagcc cgagacgaag cgcgcagcga
     1141 ggccgagctg acgcaggatc tgcaccagca gccagctggt gtcgcggcac gagccggagg
     1201 ccttctccag cgtttcctcc ggcgtctgca cgccgggctc catgcggatc aggtacctgg
     1261 tctgctgctg cagcgcacgg ttgagatcga ccaggaaatc gaccgatttc actttctccc
     1321 ggcgcggcag ggccgccagg tatttcttca gcagcgggcc aaccggggcc gcgcgcatgt
     1381 agggcttgag gtcttccttc agcgcggctt cgtactcgaa cgggaaaatt tccgcggccg
     1441 gctcgacgaa gaaatcgaag gggttataga ccgccatgtc ggcagtgagg tcaacggtga
     1501 cgcagaactc cgtgacctgg tccgggaaca ccacgcgcgc cagccagttg ccgtgcgggt
     1561 cctgctgcca gttgatgaag tgtttctccg gctcgacctt gagcgagtag ccgatgacgg
     1621 gcgtgcgcgc gtgcggcgcc gggcgcaggc ggatcagctg cggcgacagc gacaccggcc
     1681 ggtcgtagcg gtagcgggtg acgtgattga gggcggcgtg cagggtcatg cgtcagtcgt
     1741 tctggctgcg gcgaatcagc ggcaagagct atgccaccta ccctctgttc gccgggtctg
     1801 ggatactgcc cagtatgaag tcttctgcgc aacccacgct cgacagcacg tccgacttca
     1861 ttcccatccg caatctgaaa taccacgtgc gccgctgggg cgcgcgcgac aagcccatgc
     1921 tgtttctcac gcatggctgg ctcgacgtgt ccgagaccat gcacctgatg gtgcagccgc
     1981 tgctggcgcg cttccaggta ctggcgccgg actggcgcgg cctcggcctg acggagtggc
     2041 cgcaggacgg ctactggttt cccgactatg tcggcgacct ggaagcagtc atcgaccact
     2101 actcgcccgc ccaacccgtc ctgctggccg gccacagcat gggcgcgcag gcggccagcc
     2161 tgtacgcggg cctgcgggct acacgcgtgg ccaagctcat cctgatggac gcgctgttcc
     2221 tgcccgacat gccggcggaa ctggcgccgc gccgttaccg cgactggctg gaggacctca
     2281 aggacctgcc gcagccactg cactacgcga gcttcgaaga actggcgcag cgggtgaaga
     2341 agaagcatcc gcagctgagc gacgagcgcg cgctgttcgt ggcacgctgc tggggccgcg
     2401 cgcgcgacga cggccgcgtg cacctgctgg ccgatcccaa gcatcggctg aacgggccgg
     2461 gcctgttccg cgtggccgag tccatcgaga tctggaaaca ggttgccgcg cccacgctgt
     2521 tcctcgacgc cggcaaatcg atcttccgcc aggcgctgtc gaaagaggaa ctcgagcgcc
     2581 gccgctcctg cttccgcggc cgccatgagc aggtgatcga gcaggccggc cacatgctgc
     2641 atttcgacga gccggaagcc accggccgcg ccatgctgga attcctggtg aacggatgag
     2701 cacgcccaat cccgccgcgc ccaagcccgc gcacccgcat tgcctgatcg tcggcgtcgg
     2761 ccccggcatc ggccaggcgc tggcgctggc cttcgcgcgc gagaactaca acgtgtcgct
     2821 gctggcgcgc aacccggaaa agctggccga caccgtggcc atggtcaggc agaagaccgc
     2881 gcgcgatgca cgcgcctttc gcgccgacgc cggcgtggaa gcctcgctca aggccgcgat
     2941 ggtgcatgcg cggcgcgcgt tcggtgaact cgacgtgctg atcttcaacg ccgcacatgc
     3001 cgagcgcggc aagccgacca cgctgtcggt cgagcagctg gtgagcaact tcaccaccaa
     3061 cgtcggcggc gcgctggtgg cggcgcgcga agcggccaac gacatgcggc gcaagaaggc
     3121 gggttcgatc ctgttcaccg gcggcggctt cgcccacgag ccggcggccg aatacgcctc
     3181 gctgtcgctg gacaaggccg cgctgcgcag cctcacctac acgctggcgc aggagctggg
     3241 cggcgacggc atccacgtgg ccaccgtcac cgtctatggt ttcgtgcaga ccggcacgcg
     3301 cttcgacccc tcgcgcatcg cgcagcagtt cgtcgcgctg caccgccagc cgaaagggca
     3361 tttcgatacg gaagtggtct tcaagtgacc tgcgcggacg cgcggatcac tccatgcgaa
     3421 acaaaaaacg ggtccaccgc acccgctttt tgtttgttag taattatcgt ataattatta
     3481 aaacgtacaa tctttaataa caggccaaac cgccctgcgg tgaggcagaa tttcgcccgt
     3541 gcaagccttc cccctcgtcc gcaacacgtc ccggctgcag gaccgctttg acctgctggt
     3601 catcggcggc ggcatctacg gcgcgtggac c
//
LOCUS       DASZZC010000181         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1040424,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000181 DASZZC010000000
VERSION     DASZZC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040424"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1228)
                     /gene="queA"
                     /locus_tag="VHE37_03975"
     CDS             complement(161..1228)
                     /gene="queA"
                     /locus_tag="VHE37_03975"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HVT34711.1"
                     /translation="MGSIPTFGTTSMQLSDFDYDLPPELIAQQPAAQRSASRLLHLDA
                     AGAHDRMIRDLPQRLRPGDLLVFNDTRVIPARVHGRKESGGKVQLLLERITGEQEFVA
                     QVGASKTPKPGTLIQVGETTLETLGRDDDLFRLRVCGAMGAQEFFERHGELPLPPYIT
                     HKPDAADAERYQTVFAAQTGAVAAPTAGLHFDQPLLDALRDRGVETAQLTLHVGAGTF
                     QPVRVDDLAQHRMHAERFLISEALCAALAACRQRGGRVVAVGTTVTRALESAVAEDGT
                     VRAGAGETRIFITPGFRFRAIDLLLTNFHLPRSTLLMLVSAFGGHARMLDAYRHAVQQ
                     RYRFFSYGDAMLIEPEAARGG"
     gene            complement(1201..1287)
                     /locus_tag="VHE37_03980"
     tRNA            complement(1201..1287)
                     /locus_tag="VHE37_03980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1251..1253),aa:Leu,seq:caa)
     gene            complement(1367..>1983)
                     /locus_tag="VHE37_03985"
     CDS             complement(1367..>1983)
                     /locus_tag="VHE37_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34712.1"
                     /translation="APAEAAPAADSTASSGSDSSSSGSSETASSDSGSAPSETPANPH
                     KLYLGAEYNRLTLSISDPAMATNFGGSTFTTRMYTVRAGIRVFDVMGLELQGGIKGDN
                     GNDPGKAELSSYYGAFFVPTGNVFDLFEVSATLGYNAFTLSRGNASVKFARPAFGVNA
                     ELPIREISESLPDLRFTAGYKVYYEEHDSRVYGFHAGLRYDFQL"
BASE COUNT          328 a          638 c          690 g          327 t
ORIGIN      
        1 atgccgatga tcagcgccgc ttcaagcact tcacggaaaa cgacgacggc ggtggaggcg
       61 tcgaacatgc gctgcacggg accggtggat cgcccgcagg cgcgggcaca tctaaatgat
      121 aattgttctc aataagcaac tcaaccgccg gcgctgccgt tcagccgccg cgcgctgcct
      181 ccggctcgat cagcatggcg tcgccgtagc tgaagaaccg gtagcgctgc tgcacggcat
      241 ggcggtacgc atcgagcatg cgtgcgtggc cgccgaatgc gctcaccagc atcagcagcg
      301 tgctgcgcgg caggtggaag ttggtcagca gcaggtcgat ggcgcggaag cggaagcctg
      361 gcgtgatgaa gatgcgcgtc tcgccggcgc cggcacgcac cgttccatct tcggccaccg
      421 ccgattccag cgcacgcgtc accgtggtgc cgaccgccac cacgcggccg ccgcgctgcc
      481 ggcacgcggc gagcgcagcg cacagcgcct ctgatatgag aaagcgctcg gcgtgcatgc
      541 ggtgttgggc gagatcgtcc acgcgcaccg gctggaacgt gccggcaccc acgtgcagcg
      601 ttagctgcgc agtttccacc ccgcggtcgc gcagtgcgtc gagcaggggc tggtcaaaat
      661 gcaggcccgc ggttggcgcc gccaccgcgc cggtctgcgc tgcgaacacg gtctggtagc
      721 gctcggcgtc ggcggcatcg ggtttgtgcg tgatgtatgg cggcagcggc agctcaccat
      781 ggcgctcgaa gaattcctgc gcgcccatgg caccgcacac gcgcaggcgg aacaggtcgt
      841 cgtcgcggcc cagggtttcc agcgtggtct cgccaacctg gatcagcgtg ccgggtttgg
      901 gcgtcttgct ggcgccgacc tgcgcgacga attcctgctc gccggtgatg cgctccagca
      961 gcagctgcac cttgccgccg gattccttgc gcccgtgcac gcgcgcgggg atcacgcgcg
     1021 tgtcgttgaa caccagcagg tcgccgggcc gcagccgctg cggcaggtcg cggatcatgc
     1081 ggtcgtgcgc gccggcagcg tcgaggtgca gcaggcgcga ggcgctgcgc tgcgcggccg
     1141 gctgctgggc gatcagctcc ggtggcaggt cgtagtcgaa atcggaaagc tgcatggagg
     1201 tggtgccgaa ggtgggaatc gaacccacac acccttgcgg atacgagatt ttgagtctcg
     1261 cgcgtctacc agttccacca cttcggcgcg ggcgcgcatt atagcggcgc aattccaggc
     1321 aacaaaaaag gcggcccggg gccgcctttt tgcgcctgcc tcgcgctcaa agctggaagt
     1381 cgtagcgcag gccggcgtgg aagccgtaga cgcgcgaatc gtgctcttcg tagtagacct
     1441 tgtagccggc ggtgaagcgc aggtccggca gcgattcgga aatctcgcgg atcggcaact
     1501 cggcattcac gccgaacgcc gggcgcgcga acttcaccga cgcattgccg cgcgacagcg
     1561 tgaaggcgtt gtagccgagc gtggcggaca cttcgaacag gtcgaacacg ttgccggtgg
     1621 gcacgaagaa cgcgccgtaa tagctggaca gttcagcctt gcccggatcg ttgccgttgt
     1681 cgcccttgat gccgccctgc agttccagcc ccatgacgtc gaacacgcgg atgcctgcgc
     1741 gcaccgtgta catgcgcgtg gtgaaggtgc tgccgccgaa gttcgtcgcc atggcgggat
     1801 cggaaatcga cagcgtcagg cggttgtact cggctccaag gtagagcttg tgcggattgg
     1861 ccggcgtttc cgacggcgcg ctgccactgt cgctgctggc ggtttcgctg gaaccggaag
     1921 acgacgaatc gctgcccgac gaagccgtgc tgtcggcagc cggcgcagct tctgccgggg
     1981 ccg
//
LOCUS       DASZZC010000182         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_849897,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000182 DASZZC010000000
VERSION     DASZZC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849897"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VHE37_03990"
     CDS             <1..853
                     /locus_tag="VHE37_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HVT34713.1"
                     /translation="QPCTGDRATNLKMSEDGIRAAHAGGARLVLLQELHTSLYFCQHE
                     STALFDLAEAIPGPSTEWLGALAKELGVVIVGSLFERRAAGLYHNTAVVLERDGTLAG
                     KYRKMHIPDDPGYYEKFYFTPGDLGFRPIETSVGKLGVLVCWDQWYPEAARLMALAGA
                     DLLLYPTAIGWNPTDAADEQTRQRDAWVTIQRAHAVANGLPVLVANRVGFEPDPSGQT
                     VGAEFWGHSFIAGPQGEFIIKAGDKADVLVADVDLARSENVRRWWPFLRDRRIDAYDD
                     LLTRFRD"
     gene            1103..1594
                     /locus_tag="VHE37_03995"
     CDS             1103..1594
                     /locus_tag="VHE37_03995"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HVT34714.1"
                     /translation="MKATPALTMTLALLLAASAAADDGPARVTQLQWMTGCWGFSGSG
                     SSYEEVWLAPTGNSMLGLSRRISDDNYTREFEFLRVVTSGGGGFDYVAQPQGQPPTKF
                     NMLELKGTRVVFENPDHDFPQRIIYEFHAPDQLNARIEGKDPGSGQQTGINFPLRKKT
                     CGS"
     gene            1617..2369
                     /locus_tag="VHE37_04000"
     CDS             1617..2369
                     /locus_tag="VHE37_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVT34715.1"
                     /translation="MKPVKDLVTPQEWQLRVDLAAAYRLVAHFGWSDLVFTHISARLP
                     GSDHHFLINPYGMLFDEITASSLVKIDLEGKKVMDSPYPVNPAGFTIHSAVHSAREDV
                     QCVLHVHSINGVAVSAQKSGLLPISQQSLFVLASLGYHDYEGVALNEDEKPRLVRDLG
                     DKTFLMLRNHGLLTAAPTIADAFLSMYLFEAACMIQVRAQSGGGDLVQIPSPILAGIQ
                     AQANTVTRGLGGALVWPGLLRKLDRMDASFRD"
BASE COUNT          597 a          934 c          882 g          596 t
ORIGIN      
        1 gcaaccctgc accggcgacc gtgccaccaa cctgaaaatg tccgaggatg gcatccgcgc
       61 cgcgcacgcc ggcggcgcgc gcctggtgct gctgcaggaa ctgcacacct cgctctactt
      121 ctgccagcac gaatcgacgg cactgttcga cctggccgag gccatccccg gcccgtccac
      181 tgaatggctc ggcgcactgg cgaaggaact cggcgtggtc atcgtcggct cgctgttcga
      241 gcgccgcgcc gccggcctgt accacaacac cgcggtggtg ctggaacgcg acggcacgct
      301 cgccggcaag taccgcaaga tgcacatccc cgatgacccc ggttactacg agaagtttta
      361 cttcacgccc ggtgacctag gcttcaggcc catcgagacc agcgtcggca agctcggcgt
      421 gctggtgtgc tgggaccagt ggtacccgga agccgcgcgg ctgatggcgc tggccggcgc
      481 cgacctgctg ctgtacccga ccgccatcgg ctggaatccc accgatgccg cggacgagca
      541 gacgcgccag cgcgatgcgt gggtcaccat ccagcgcgcg cacgcggtgg ccaatggatt
      601 gccggtgctg gtggccaacc gcgtcggctt cgagcccgat ccatcggggc aaaccgtcgg
      661 cgcggaattc tggggccaca gtttcatcgc cggcccgcag ggcgagttca tcatcaaggc
      721 cggcgacaag gctgacgtgc tggtggccga tgtcgatctc gcgcgcagcg aaaacgtgcg
      781 gcgctggtgg cccttcctgc gtgaccgtcg catcgatgca tacgatgatc tgctcacgag
      841 atttcgagat taggccgccg ctggtggcgt gcgcggggcg aatgttcact ggacattcgc
      901 cttttccgcg cactctgcgc gaccgtcgca tcgacgcgta cggcgatctg gtgcgtcgtt
      961 attgcgacga atgaggcgca aaatcacctc caccgcaccg ctgcttggct tgttattaat
     1021 tatcgcttat ttatagttta attatcgttt ttataacatc aaaacgggcc gcgcggtgga
     1081 ggcattttct ggagcaaaag caatgaaagc gactcccgca ttgaccatga cgctggcctt
     1141 gctgctcgcc gccagcgcag ctgccgatga tggtccggcc cgcgtcaccc agctgcagtg
     1201 gatgaccggc tgctggggtt tctccggcag cggcagcagc tacgaggaag tgtggctggc
     1261 gcccaccggc aacagcatgc tcggcctgtc gcggcgcatc tccgacgaca actacacgcg
     1321 cgaattcgag ttcctgcgcg tcgtcacctc cggcggcggc ggcttcgact acgtggccca
     1381 gccgcagggc cagccgccca ccaagttcaa catgctcgaa ctcaagggca cgcgcgtagt
     1441 gttcgaaaat cccgatcacg attttccgca gcggatcatc tacgagttcc acgcgcccga
     1501 tcagctcaat gcgcgcatcg aaggcaagga ccccggcagc ggccagcaga ccggcatcaa
     1561 tttcccgctg cgcaagaaaa cgtgcggttc ctgaacgcca cctggagaac gctatgatga
     1621 agccagtaaa agacctggta accccgcagg aatggcagct gcgtgtggat ctcgccgccg
     1681 cataccggct ggtcgcccac ttcggctgga gtgacctggt cttcacccac atctcagcgc
     1741 gcctgcccgg ctccgatcac cacttcctga tcaatcccta cggcatgctg ttcgacgaga
     1801 tcaccgccag ttcgctcgtc aagatcgacc ttgaaggcaa gaaggtgatg gactcgccgt
     1861 acccggtgaa tcccgccggt ttcaccatcc acagcgccgt gcattccgcc cgcgaagacg
     1921 tgcaatgcgt gctgcacgtg cattccatca acggcgtcgc cgtctccgcg cagaagagcg
     1981 gcctgctgcc catctcgcag caatcattat tcgtgctcgc ctcgcttggc taccacgact
     2041 acgaaggcgt agcgctcaac gaggacgaaa agccgcgact ggtgcgcgac ctcggcgaca
     2101 agaccttcct catgctgcgc aaccacggcc tgctcaccgc cgcgcccacc attgccgacg
     2161 cgttcctctc catgtacctg ttcgaggccg cctgcatgat ccaggtgcgt gcgcagtccg
     2221 gcggcggcga cctggtgcag attccgtcac caatccttgc ggggattcag gcgcaagcca
     2281 ataccgttac gcgtggtcta ggcggtgcgc tggtgtggcc ggggttgttg cgcaagctgg
     2341 atcggatgga tgcgtcgttt agggattgag tcgcttcagt gccttgagcg aaatgccagc
     2401 gtcaacctct ttcagaatct cgaagatttg ctcatcgtta tcccgcatct aacgtccgac
     2461 ctccaagggt cggactccat tccatggtcc tactcaatga gcgcaggtga gtgcgaaggc
     2521 aaatgtgcgg gtgagggcac gcgtgctcgt gcgcacgggc gcgggtacgc aatgcgaaaa
     2581 caaaagccgc ccaggcaggg cggctttgtt ttgcagcgcg ctgacgattg aaaaattcat
     2641 cagcgcgaga cttcttaaaa agccttccga gggccgaaaa caggcccaaa accctcgaag
     2701 cgccttggca tttttaatgt tataaaatat ttatacattt taattatact gtattatttt
     2761 caacataaaa atgagaggcg cgtttggggt ctttggcggg ttttgggtca aagttcggtt
     2821 cgtatccgcg cagcacgaaa atcattttga cggtgcagta ggcgcatcag aagatttttc
     2881 ggctttgatg cgttgttcga tgtcgtcgcc gtaatagtgg gggaaccctt tcaggaccgg
     2941 gtcccaatga gtaatttcag aaagcgattt caaaattaaa tcgtcgtcga agaacaggag
     3001 taccaaata
//
LOCUS       DASZZC010000183         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1170982,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000183 DASZZC010000000
VERSION     DASZZC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170982"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VHE37_04005"
     CDS             <1..329
                     /locus_tag="VHE37_04005"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HVT34716.1"
                     /translation="ANHIAPEHLELAVEHPRALLPRLRHAGAVFLGRHTPEAFGDYCA
                     GPNHVLPTSRAARFSNPLGVYEFVKRTSLIECTPAGARVLAPIAGTMADAEGLQAHAA
                     SARDRL"
     gene            419..1135
                     /gene="aqpZ"
                     /locus_tag="VHE37_04010"
     CDS             419..1135
                     /gene="aqpZ"
                     /locus_tag="VHE37_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HVT34717.1"
                     /translation="MKSYGAEFFGTFWLVLGGCGSAVLAAAFPAVGIGLLGVSLAFGL
                     TVLTMAYAIGHISGCHLNPAVSLGLWAAGRFPAQKVAPYVIAQVLGAIAAGGVLFVIA
                     SGAPGFDAAKGFASNGYGAHSPGGYSLLAALVTEVVMTTFFLLVILGATDQRAPAGFA
                     PIPIGLALTLIHLISIPVTNTSVNPARSTGVAVFVGDWAVGQLWLFWMAPMIGALLGA
                     WIYRWLAEPEMVTAASAAKA"
     gene            complement(1148..1909)
                     /locus_tag="VHE37_04015"
     CDS             complement(1148..1909)
                     /locus_tag="VHE37_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT34718.1"
                     /translation="MRIVSHTCSNTEIVCALGCGNLLVGIDQDSDFPEDPCARLPKLG
                     RDLELDIEATRALRPDLVLTSLTVPGHERIVDALRAAGLPMVVIDPIGLDGVYASIIE
                     IAEALGVLARGRTLVAQMQNAMPPAPRRGIKVLVEWWPKPVIAPARLSWVNELIELAG
                     GVNPCAALDGKSAEIGDEAARTAAPDAIVMSWCGVKEEKYRADVVLRRPGWSDIPAVK
                     NARVYPVTEAFLGRPGPRLVQGYEALRHIVARCAA"
     gene            complement(1906..2331)
                     /locus_tag="VHE37_04020"
     CDS             complement(1906..2331)
                     /locus_tag="VHE37_04020"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVT34719.1"
                     /translation="MAVHEVRIEEDFAAPREKIFAFLTEPSKLTKVWACRFERTRDGE
                     GEPNGVGSIRRIKVAGVAFEETVTAYQRPSLIEYVKTTGDATKGHKGRMEFTDLPDGG
                     THLVYTMSFEPRVALAGRVFAGLLKARWNKGVVNILPNL"
     gene            2400..2774
                     /gene="rnpB"
                     /locus_tag="VHE37_04025"
     ncRNA           2400..2774
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHE37_04025"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2977..>3202
                     /locus_tag="VHE37_04030"
     CDS             2977..>3202
                     /locus_tag="VHE37_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34720.1"
                     /translation="MFAGSHQLTVDEKGRLAIPSRFRAPLAERCQSQLVITMGHESCL
                     EIYPAPEFARIASDIDNMDNRDHAELLKRAF"
BASE COUNT          523 a         1029 c         1072 g          578 t
ORIGIN      
        1 tggccaacca catcgcaccg gaacacctgg agctggcagt cgagcacccg cgcgcgctgc
       61 tgccgcgcct gcgccacgcc ggcgcggtgt tcctcggccg gcatacgcca gaagcgttcg
      121 gcgactactg cgccgggccc aaccacgtgc tgccgacctc gcgcgccgca cgattttcca
      181 atccgctggg cgtgtacgaa ttcgtcaagc gcaccagcct gatcgagtgc acgcccgctg
      241 gcgcgcgcgt gctggcgccc atcgccggca ccatggcgga cgcggaaggc ctgcaggcgc
      301 acgcggccag cgcacgcgac cgtctctgac acgccgctgt cggaatcttc cgcttgtcag
      361 cacgttttcg cgggcgtcta atcggacgat ccttgggagt gctgacagga gccgcaaaat
      421 gaaaagctac ggcgcggagt ttttcgggac attctggctg gtgcttggcg gctgcggcag
      481 cgcggtgctg gccgcggcat tcccggcggt tggcatcggg ctgctcggcg tttcgctggc
      541 attcggcctc accgtgctga ccatggccta tgccatcggc cacatttcgg gctgtcatct
      601 caaccccgcc gtgtcgctcg gcctgtgggc cgccggcagg ttccccgcgc agaaagttgc
      661 gccgtacgtg atcgcgcagg tgcttggcgc catcgcggcg ggcggcgtgc tcttcgtcat
      721 cgccagcggc gcgcccggct tcgacgcggc caagggtttt gcttcgaacg ggtatggcgc
      781 gcattcgccc ggcggctact cgctgctggc ggcgctggtc accgaggtgg tgatgaccac
      841 tttcttcctg ctggtcatcc tcggcgcgac cgaccagcgc gcgccggcgg gattcgcgcc
      901 gattccgatc gggctggcgc tgaccctgat ccacctgatc agcattccgg tgaccaacac
      961 ctcggtgaac ccggcgcgca gcaccggcgt ggcggtgttc gtcggcgact gggcggtcgg
     1021 ccagctgtgg ctgttctgga tggcgccgat gatcggcgcg ctgcttggcg cctggatcta
     1081 caggtggctg gcggagcccg agatggtgac ggcggcgtct gccgccaagg cctgacggcg
     1141 cggccggtca ggcggcgcag cgcgcgacga tgtggcgcag cgcctcgtaa ccctgcacca
     1201 gccgcgggcc ggggcggccg aggaaggctt cggtcaccgg gtacacgcgc gcgtttttca
     1261 ccgcgggaat gtcactccag cccgggcggc gcagcacgac gtcggcgcgg tatttctctt
     1321 ccttcacgcc gcaccagctc atgacgatgg cgtccggcgc ggccgtgcgc gccgcctcgt
     1381 cgccgatttc cgcgctcttg ccgtcgagcg ccgcgcaggg gttgacgccg ccggcgagct
     1441 cgatcagctc gttcacccag gacagccgcg ccggcgcgat caccggcttg ggccaccatt
     1501 ccaccagcac cttgatgccg cgccgtggtg ccggcggcat ggcgttctgc atctgcgcca
     1561 ccagcgtgcg gccgcgcgcc agcacgccga gcgcttcggc gatttcgatg atgctggcgt
     1621 agacgccatc gaggccgatg ggatcgatca ccaccatcgg caggccagcc gcacgcaagg
     1681 cgtcgacgat gcgttcatgg cccggaacgg tgagcgaggt cagcaccagg tcaggcctga
     1741 gtgcacgcgt ggcctcgata tcgagctcga gatcacgccc gagcttgggc aggcgcgcgc
     1801 aggggtcttc gggaaaatcg gaatcctggt cgatgccgac gagaagattt ccgcagccga
     1861 gcgcgcagac gatctcggtg ttggagcagg tgtggctgac gattctcaaa gattcggcag
     1921 gatgttgacc acgcccttgt tccagcgcgc cttgagcagg ccggcgaaca cgcgcccggc
     1981 cagcgccacg cgcggctcga agctcatggt gtacaccagg tgggtgccgc cgtcgggcag
     2041 atcggtgaat tccatgcggc ccttgtggcc cttggtggca tcgccggtgg tcttgacgta
     2101 ctcgatcagc gacggccgct ggtaggcagt gacagtctct tcgaaagcaa ccccggccac
     2161 cttgatgcgg cggatggagc ccacgccgtt gggctcgcct tcgccgtccc tggtgcgctc
     2221 gaagcggcag gcccagacct tggtcagctt ggatggctcg gtgaggaagg caaatatttt
     2281 ttcacgcgga gcagcgaaat cttcctcgat gcggacctcg tggacggcca ttgggatcct
     2341 ccttggaaat gatggcgaat gtagcagagc tttttctcca ttcgcgcgat aatgggcgcg
     2401 gagtcggccg aacaatcgcc gccacggcag atctaggtct gcagtggggg aggaaagtcc
     2461 gggctccgca gggcagggtg ccaggtaacg cctgggggcc gcgaggccac ggatagtgca
     2521 acagaaagat accgccgaat tcttcgcaag aaggatggca agggtgaaat ggtgcggtaa
     2581 gagcgcaccg cgagtctggc aacagaccgg cacggcaaac cccacctgga gcaaggccaa
     2641 atagggaagg gcgccttcac tggcgacgcg tggcccgcgt gccttccggg taggctgctt
     2701 gagcgcggcg gcgacgtcgc gcctagagga atggttgttc tcgacagaac ccggcttaca
     2761 ggccgactcc ttttttcctc tcaaatcccg cgctgtggac aacttctgcg gccaccttaa
     2821 aaaccggttg taagtggctg tttttccggt gagtcaaaca aataaataac ttcctgtttt
     2881 gtctagggtt tcacacgatc tcgtgcttgc ccctgattca tcggtggttt atagtgcata
     2941 aaggtgggga aaagtggatt gagaatccaa taaaccatgt tcgcgggctc gcatcagctg
     3001 acggtcgacg aaaaggggcg cctggcgatc ccttcgcgct tccgtgcgcc gctggccgag
     3061 cgttgccagt cgcagctggt gatcaccatg ggccacgaat cctgcctgga gatttacccg
     3121 gcgccggagt tcgcgcgcat cgcctccgac atcgacaaca tggacaaccg cgatcacgcg
     3181 gaactgctca agcgcgcctt cg
//
LOCUS       DASZZC010000184         4369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_17117,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000184 DASZZC010000000
VERSION     DASZZC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17117"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1377)
                     /locus_tag="VHE37_04035"
     CDS             complement(43..1377)
                     /locus_tag="VHE37_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34721.1"
                     /translation="MTTHAKATRIELRDFSTAPMRAFHLSWIAFFVCFFAWFACAPLM
                     PVIKGVFNLTKDQIANISIAAVAVTIVVRMLVGPLCDRYGPRKTYTGLLLIGALPVLG
                     VASAQSYESFLFFRLAIGAIGASFVITQYHTSVMFAPNVVGTANAAAAGWGNSGGGAT
                     QTLMPLLLAALLYLGADGAYGWRVALLVPGVMMLVTAYFYWRCTQDTPAGNYAELRAT
                     GLLPDAGRKAGWVSFKQACANYRVWLLFVTYGACFGVEIFIHNIAATYYVDRFGFSLS
                     AAGFAAGSFGLLALFARALGGYLSDRVARRQGLGGRTLLLFALIGCEGMGLLCFSQMD
                     IAWLAVSAMLVFGLFTHMACGATYALVPFVDRQALGGVAGIIGAGGNAGAVAAGFLLK
                     AAASTQQCLLMLGAFVTVSALCAIAVRFSAEHQAREEELFQQALAERAAAVA"
     gene            1498..1797
                     /locus_tag="VHE37_04040"
     CDS             1498..1797
                     /locus_tag="VHE37_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34722.1"
                     /translation="MRMPLFAGHFAPLCTSSCTSVVRLHRLERAHAPLWDGAACALRG
                     CDADSGGTHYGDKVDLLATYAVNSNLSLSAKYADYNADKFSVDTQKTWAYLEYRL"
     gene            complement(2032..3030)
                     /locus_tag="VHE37_04045"
     CDS             complement(2032..3030)
                     /locus_tag="VHE37_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HVT34723.1"
                     /translation="MKKTRLGYLRLCDAAPLIVARGLGCYAQAGLRVELVPLKSWAEV
                     RDRLMLGDIHAAHCLAGIPLAAQAGLFGPEAKLATAFTLSHSGNAITLATPLAQLFRT
                     GRDAFAALARERSAQGRPLTFASVFPVSKHEYELRHWWAGLGLAPEDMRLVVVSPAET
                     APSLHSGRIDGYCVGEPWNTRAIVEGWGEAVTTSAQLGLPGTEKVLAVDAGWLKAPEH
                     AALLQALTLAADWLADAANAESAMELLAPYAGRPLRELRAAICGEFRTQRNAVPAAAE
                     PFLRFDRGINRPNTAHGLWYLQQMRAADQLDPAVRMEEVCARAFRADVYDELIGAR"
     gene            complement(3017..3604)
                     /locus_tag="VHE37_04050"
     CDS             complement(3017..3604)
                     /locus_tag="VHE37_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HVT34724.1"
                     /translation="MTTTPDAGPTVLLVNGDSALADALSAAGYAVSRAGEADDLYASV
                     ERHKPDAIVVAADSPSRDVLEHLAFINRKHPQPVLLFHDGSDDALVRRAMRAGVSAYV
                     ARDLPPLALRSLIEVSALHFEQTRSLRQALSRAEKLRAERKLVDAAKCRVMETLGLGE
                     AAAYKHLQALAMNGRRSLAEVAHELLGSGGTDEKD"
     gene            3780..>4369
                     /locus_tag="VHE37_04055"
     CDS             3780..>4369
                     /locus_tag="VHE37_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019384358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34725.1"
                     /translation="MKATRIDLFSLATPQMRAFHLAWLAFFVCFFAWFAVAPLMPVIK
                     GEFNLSKDQIANINIAAVGITVLVRLLVGPLADRYGPRKVYALLLLFGSLPVFGIALA
                     RSYEAFLVARLAIGAIGASFVITQYHTSVMFAPNVVGTANAASAGWGNAGGGVTQTVM
                     PLVLAGIVGLGLLPASAWRLSMLLPGTLMLIVGTLY"
BASE COUNT          731 a         1499 c         1457 g          682 t
ORIGIN      
        1 accatgccgt ggccgatgac gacgatgttc atgccgggtc tctcaggcga ctgccgccgc
       61 gcgctcggcc agcgcctgct ggaacagctc ttcctcgcgc gcctggtgct cggccgagaa
      121 gcgcacggcg atcgcgcaca gcgcgctcac cgtgacgaag gcgccgagca tcagcaggca
      181 ttgctgcgtg ctggccgccg ccttgagcag gaagcccgct gccacagcgc cggcattgcc
      241 gcccgcgccg atgatgccgg ccacgccgcc gagtgcctgg cggtcgacga agggcaccag
      301 cgcgtaagtc gcgccgcagg ccatgtgcgt gaacaggccg aacaccagca tggcgctcac
      361 ggcgagccag gcgatgtcca tctgcgaaaa acacagcagg cccatgccct cacagccgat
      421 cagcgcgaac agcagcagcg tgcgaccgcc cagaccctgc cggcgcgcca cgcgatccga
      481 caggtagccg cccagcgcgc gcgcgaacag cgccagcagc ccgaagctgc ccgcggcgaa
      541 gccggcggcg ctgagcgaga agccgaacct gtcgacgtaa taggtcgcgg cgatgttatg
      601 gatgaagatc tccacgccga agcaggcgcc gtaggtgaca aacagcagcc acacgcggta
      661 attggcgcag gcctgcttga acgacaccca gccggccttt ctgccggcat cgggcaaaag
      721 gccggtggcg cgcagctcgg cgtagttgcc ggccggcgtg tcctgcgtgc agcgccagta
      781 gaagtacgcg gtgaccagca tcatcacgcc gggcaccagc agcgccacgc gccagccata
      841 cgcgccgtcg gcacccaaat aaaggagcgc cgccagcagc agcggcatca gggtctgggt
      901 ggcgccgccg cccgagttgc cccagccagc ggccgccgca ttcgccgtgc ccaccacgtt
      961 gggcgcaaac atcaccgagg tgtggtactg ggtgatgacg aaactggcgc cgatcgcgcc
     1021 gatggccagg cggaagaaca ggaacgattc gtacgactgc gcgctggcga cgcccagcac
     1081 cggcagcgcg ccgatcagca gcaagccggt gtaggtcttg cgtgggccgt agcggtcgca
     1141 cagtggcccg accagcatgc gcacgacgat ggtgaccgcg accgcggcga tgctgatgtt
     1201 ggcgatctgg tccttggtca ggttgaacac gcccttgatc accggcatca gcggcgcgca
     1261 ggcaaaccag gcgaagaagc agacgaagaa cgcgatccag ctcaggtgga acgcgcgcat
     1321 cggcgcggta gaaaaatcgc gcagctcgat gcgcgtggct tttgcatggg tcgtcatggg
     1381 cggttctccg gtgcaatccg atggaacggg agcagatgcc ccgcatcacc gacgcactcg
     1441 tgccgcttgc ggcctggtcc acgtcgcgtt tgaaacgtat caatcgaaaa cttagcaatg
     1501 cgtatgccac tttttgcagg gcacttcgcc cccttgtgca cctcgtcgtg caccagcgtg
     1561 gttcgcctgc accgtctgga gcgcgcgcac gctccgttgt gggatggcgc tgcgtgcgca
     1621 ttgcgcgggt gcgatgccga cagcggcggt acgcactacg gcgacaaggt cgacctgctg
     1681 gcgacttatg cggtcaacag caacctctcg ctcagcgcca agtacgcgga ctataacgcc
     1741 gacaaattct cggtcgacac ccagaagacc tgggcctacc tcgaatacag gctctaggag
     1801 acctcgatga gccaatacgg gcccgaactt gctgccgagt tctgccagcg agtagtagcc
     1861 gttgacctgc gacgacgaat cctcgtagcg cagctgccgc gcgtattccg ccgtgcaggt
     1921 cagcggaaag aacatcgcca cggtgccggc tgcatgcagg ccgagggtct tgcgtgccag
     1981 caacgcgtgt gcgacgaccc gccgcgcacc ggcgcagcgc gcggtgcgcc atcaacgcgc
     2041 gccgatcagt tcgtcgtaca cgtccgcgcg gaacgcgcgc gcacacacct cttccatgcg
     2101 aaccgccgga tcgagctggt cagcagcgcg catctgctgc agataccaga gtccgtgcgc
     2161 ggtgttgggg cggttgatgc cgcgatcgaa acgcaggaaa ggctccgctg ccgcgggcac
     2221 tgcgttgcgc tgcgtacgga attcaccgca gatggctgca cgcaattcgc gcagcggccg
     2281 tccggcatag ggcgcgagca gttccatcgc gctctccgcg ttggccgcgt cggccagcca
     2341 gtccgcggcg agcgtgagtg cctgcagcag ggccgcatgt tccggagcct tcagccagcc
     2401 tgcatcgacg gccaggactt tttccgtgcc cggcagcccg agctgcgcgc tggtggtcac
     2461 ggcttcgccc cagccttcga cgatggcgcg cgtgttccac ggctcgccca cgcaatagcc
     2521 gtcgatgcgt ccgctgtgca gcgacggcgc ggtctccgct ggcgacacca ccaccaggcg
     2581 catgtcttcg ggcgccaggc cgagcccggc ccaccagtgg cgcaactcgt attcgtgctt
     2641 ggacaccggg aacaccgagg cgaaggtcag cggccggccc tgtgcagagc gttcccgcgc
     2701 cagcgcggcg aaggcgtcgc ggccggtacg gaacagctgc gccagcggcg tcgccagcgt
     2761 gatggcgttg cccgagtggc tgagcgtgaa cgcagtcgcc agcttcgcct ccgggccgaa
     2821 cagcccggcc tgcgctgcca gcggaatgcc ggcaaggcag tgcgcggcgt ggatgtcgcc
     2881 gagcatcagc cggtcgcgca cttcggccca tgatttcagc ggcaccagtt ccacgcgcag
     2941 gcccgcctgc gcgtagcagc ccagtccgcg cgcgacgatc agcggcgcgg cgtcgcacag
     3001 ccgcaggtag ccgagcctag tctttttcat cggtgcctcc ggagccgagc agctcatgcg
     3061 caacttcggc cagcgagcgc cggccgttca tggccagcgc ctgcaggtgc ttgtatgccg
     3121 cggcctcgcc caggcccagc gtttccatca cgcggcactt ggccgcatcc accagcttgc
     3181 gttcggcgcg cagtttctcg gcgcgcgaca gcgcctgcct gagcgagcgc gtctgctcga
     3241 aatgcagcgc cgaaacctcg atcagcgagc gcagcgccag cggcggcagg tcgcgcgcga
     3301 cgtaggcgct gacgccggcg cgcatggccc ggcgcaccag cgcgtcgtcg ctgccatcgt
     3361 ggaacagcag caccggctgc ggatgcttgc ggttgatgaa cgccaggtgc tcgagcacgt
     3421 cgcgcgacgg cgaatcggcc gccaccacga tggcgtcggg cttgtgccgc tcgacgcttg
     3481 catacaagtc gtcggcttca ccggcgcgcg aaacggcata gcccgccgcc gacagcgcgt
     3541 cggccagcgc gctgtcgccg ttgaccaaca gcacggtcgg tccggcatcg ggtgtggtgg
     3601 tcatgcgcgg ctaaaccagc aacggcgatg ccaacacgcg tcaccacccg cggcggaaaa
     3661 aacatggcat gcactttgct ccgtttgttt tgccggcagt gacgccggaa agcggcctgt
     3721 ccgctcgcgg tcaatgacgt ccgccccttg atctcttcgg agcacggagt gcgcacgtca
     3781 tgaaagcgac caggatcgat ctgttttccc ttgccacgcc gcagatgcgc gcgttccacc
     3841 tggcatggct ggcgttcttc gtctgcttct tcgcctggtt tgcggtggcg ccgctgatgc
     3901 cggtaatcaa gggcgagttc aacctttcca aggaccagat cgccaacatc aacatcgccg
     3961 cggtgggcat caccgtgctg gtgcgcctgc tggtcggccc gctggccgac cgctacggtc
     4021 cgcgcaaggt ctacgcgttg ctcctgctgt tcgggtcgct gccggtgttc ggcatcgccc
     4081 tggcgcgcag ctatgaagcc ttcctggtgg ctcggctggc gatcggagcc atcggcgcca
     4141 gcttcgtcat cacccagtac cacacctcgg tgatgttcgc gcccaacgtg gtgggcaccg
     4201 ccaatgcggc gtcggccggc tggggcaacg ccggcggcgg cgtgacgcag acggtgatgc
     4261 cgctggtgct ggccggcatc gtcgggctgg ggctgttgcc ggcctcggcg tggcggctgt
     4321 cgatgctgct gccaggcacg ctgatgctga tcgtcggcac gctttactg
//
LOCUS       DASZZC010000185         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_978007,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000185 DASZZC010000000
VERSION     DASZZC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978007"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VHE37_04060"
     CDS             <1..221
                     /locus_tag="VHE37_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34726.1"
                     /translation="LLLPTVLLWMSTVYLRYHYVVDLAGGLLVCLAGIAASRLYERSE
                     LATRINCAATNSVARSAEAKNVSTAPHI"
     gene            complement(307..2385)
                     /gene="ppk1"
                     /locus_tag="VHE37_04065"
     CDS             complement(307..2385)
                     /gene="ppk1"
                     /locus_tag="VHE37_04065"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HVT34727.1"
                     /translation="MTSNPPPMDLQPAELFLNRELSLLEFNRRVLELARDESVPLLER
                     LKFLCISSSNLDEFFEIRVAGLQQMAELQPTQRSPDGMTAAENLTAISTRAHVLVDEQ
                     YKLFNDVLIPALEKEKIRFLRRTEWTAEQDAWLKSYFGSELAPVLSPLGLDPAHPFPR
                     ILNKSLNFIVSLSGKDAFGRNTGFAIVQAPRALPRLIRIPREIAGSGPHDFVFLSSVI
                     HAYIDELFPGMEASGCFQFRVTRNSDLLVDEEEAGDLLAALEGELSQRQWGDTVRLEV
                     AHNCPERMVAYLTDVMQLTPADVYQVNGPVNLNRLIAIPDMVDRADLKYAPYVPRLPK
                     ALQVADPFAAIRERDILLQHPYDSFVPVIDFLRAAARDPRVLAIKQTLYRTGDKSAVA
                     DALTEAALAGKEVTVVVELRARFDEENNIDLAEKLQEAGAHVVYGVVGYKTHAKMSLV
                     VRREDKGLTYYAHLGTGNYHPRTARQYTDYGLFTADSGICKDVHAVFLQLTSLGKTTR
                     LKHLLQSPFTLAKAMHAKIEREIEHAKKGGKGGRIIAKLNALVEPEIIQALYRASAAG
                     VSIDLIVRGMCSLRPGVKGLSDHIRVRSVAGRFLEHQRVIYFGNGGDAELYLSSADWM
                     ERNFFRRVEVAFPVLDKKLRERIVEHLNGYLADTAQSWLLQADGSYQRPEKIEGKAIQ
                     QRLMEEAAPA"
     gene            complement(2565..3236)
                     /locus_tag="VHE37_04070"
     CDS             complement(2565..3236)
                     /locus_tag="VHE37_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT34728.1"
                     /translation="MSKRLTSTRRQRRELKIPAPSRKGNIKIPLAGWISCARVVLIEE
                     GIGAVKVDRLAKRLNVTRGGFYYHFNQHHKLLEALLDDWRRHNCFTPAQIDASSPAAA
                     LEALQQISDNLVHERGFDPQYDMAIREWARISQPVADVVHEVDEQRVEVLRRIFSGLG
                     CKPKEAAIRARVYYWHQIGYYAIGVREDTGQRESNLQLYLEVLGGEKYLEAIAGSRRS
                     SAAAA"
     gene            3395..>3841
                     /locus_tag="VHE37_04075"
     CDS             3395..>3841
                     /locus_tag="VHE37_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT34729.1"
                     /translation="MIGTDVLIIGGGIAGASLGYALARPQDAVLLEAEDQPGYHSTGR
                     SAALYMTTYGHPLIRALTHASRAFLERPPAGFAEVALMQLRGVLWIARADQQAAFARY
                     YADCSALDRSVARCTVEQALALCPALQSAYVNQAFVEPSAQDMDVSA"
BASE COUNT          571 a         1279 c         1305 g          686 t
ORIGIN      
        1 ggctgctgct gcccaccgtg ctgttatgga tgtctacggt gtacctgcgc taccactacg
       61 tggtcgatct ggccggcggg ctgctggtat gcctcgcggg catcgcggcc tcgcgcctgt
      121 atgagcgttc ggaactggcg acgcggatca attgcgctgc tacgaattcc gtagcacgga
      181 gcgcggaggc gaaaaatgtc tccaccgcac cacacatcta gatgttatta attattatct
      241 attttataaa atagataact ttttataaca cgccacggag cggtgcggtc aggcaaaaaa
      301 accgcgtcag gccggcgccg cctcttccat cagccgctgc tggatcgcct tgccctcgat
      361 cttctccggc cgctggtacg agccgtcggc ctgcagcagc cagctttgcg cggtgtcggc
      421 gagatagccg ttgaggtgct cgacgatgcg ctcgcgcagc ttcttgtcca gcaccgggaa
      481 ggccacctcc acgcggcgga agaagttgcg ctccatccag tcggcggacg acaggtacag
      541 ctcggcgtcg ccgccgttgc cgaagtagat cacgcgctgg tgttcgagga agcgcccggc
      601 caccgagcgc acgcggatgt ggtcggacag gcccttcacg ccggggcgca gcgaacacat
      661 gccgcgcacg atcaggtcga tggacacgcc ggccgcgctc gcgcggtaca gcgcctggat
      721 gatctcgggc tccaccagcg cgttgagctt ggcgatgatg cgcccgccct tgccgccctt
      781 cttcgcgtgc tcgatctcgc gctcgatctt ggcgtgcatc gccttggcca gcgtgaacgg
      841 cgactgcagc aggtgcttga gcctggtggt cttgcccagg ctggtcagct gcaggaacac
      901 cgcgtgcacg tccttgcaga tgccggagtc ggcggtgaac agcccgtagt cggtgtactg
      961 gcgcgcggtg cgcgggtggt agttgccggt gcccaggtgc gcgtagtagg tgagcccctt
     1021 gtcctcgcgc cgcaccacca ggctcatctt ggcgtgcgtc ttgtagccga ccacgccgta
     1081 caccacgtgc gcgccggcct cctgcagctt ttccgcgagg tcgatgttgt tctcttcatc
     1141 gaagcgcgcg cgcagctcca ccaccaccgt cacttccttg ccggccagcg cggcctcggt
     1201 cagcgcgtcg gccaccgcgc tcttgtcgcc ggtgcggtac agggtctgct tgatcgccag
     1261 cacgcgcggg tcgcgcgccg ccgcgcgcag gaaatcgatc accggcacga acgagtcgta
     1321 cggatgctgc agcaggatgt cgcgctcgcg gatcgccgcg aacggatcgg ccacctgcag
     1381 cgccttgggc aggcgcggca cgtacggcgc gtacttgagg tcggcgcggt ccaccatgtc
     1441 gggtatggcg atcaggcggt tcaggttcac cggcccgttc acctggtaca catccgccgg
     1501 cgtcagctgc atcacgtcgg tgaggtacgc caccatgcgc tcggggcagt tgtgcgccac
     1561 ttcgagccgc accgtgtcgc cccactggcg ctgcgacagc tcgccttcca gcgcggccag
     1621 cagatcgccg gcttcctcct cgtcgaccag caggtcggag ttgcgcgtca cgcggaactg
     1681 gaagcagccg ctggcttcca tgcccgggaa cagctcgtcg atgtaggcgt ggatcacgct
     1741 cgacaggaag acgaaatcat gcggcccgga gccggcgatc tcccgcggaa tcctgatcag
     1801 ccgcggcagc gcgcgcggcg cctgcacgat ggcgaagccg gtgttgcggc cgaaggcgtc
     1861 cttgcccgac agcgagacga tgaagttgag cgacttgttg aggatgcgcg gaaacggatg
     1921 cgccgggtcg agtcccagcg gcgacagcac cggcgccagc tcgctgccga agtagctctt
     1981 cagccaggcg tcctgttccg cggtccactc ggtgcggcgc aggaagcgga tcttctcttt
     2041 ttccagcgcc gggatcagca cgtcgttgaa cagcttgtac tgctcgtcca ccagcacgtg
     2101 cgcgcgggtg gaaatggccg tgaggttttc cgccgcggtc atgccgtccg ggctgcgctg
     2161 ggtgggctgc agttccgcca tctgctgcag gccggccacg cggatctcga agaactcgtc
     2221 gaggttggac gacgagatgc acaggaactt gagccgctcg agcagcggca cgctctcgtc
     2281 gcgcgccagt tccagcacgc gccggttgaa ctcgagcagg ctgagctcgc ggttgaggaa
     2341 cagctcggcg ggctgcaggt ccatcggcgg cggattgctg gtcatgggag aacagtaggg
     2401 cgacagcgtg aagtggctgt gacagtgcgc acggcaggag gttgcgcccc attctgcgcc
     2461 cgcgcgcggc cgcgttgcca cctcgccagc cccgggccgc aggttaagcg gttgatgttc
     2521 cgcggtcttt gacgggcgcg ccgggccgtg cccggaccac ccggctacgc agcggcagcg
     2581 ctgctgcggc gcgagccggc gatggcctcg aggtactttt cgccgccgag cacttccagg
     2641 tacagctgca ggttgctctc gcgctggccg gtatcttcgc gcacgccgat ggcgtagtag
     2701 ccgatctggt gccagtagta gacgcgcgcg cggatcgccg cttccttcgg cttgcagccc
     2761 agcccgctga agatgcggcg cagcacctcg acgcgctgct cgtccacttc atgcaccacg
     2821 tcggccaccg gctgcgagat gcgcgcccac tcgcggatgg ccatgtcgta ctgcgggtcg
     2881 aagccgcgct cgtgcaccag gttgtcgctg atctgctgca gggcctccag cgccgcggcg
     2941 ggcgaggagg cgtcgatctg cgccggcgtg aagcagttgt gccggcgcca gtcgtcgagc
     3001 agcgcttcca gcagcttgtg gtgctggttg aagtggtagt agaagccgcc gcgcgtcacg
     3061 ttcaggcgct tggccaggcg gtcgaccttc accgcgccga tgccttcctc gatcagcacc
     3121 acgcgcgcgc agctgatcca gccggccagc ggaatcttga tgttgccctt gcgcgacggc
     3181 gccgggatct tgagctcgcg ccgctggcgg cgggtggagg tgaggcgctt gctcatgtgc
     3241 gggctcttgt tcttgaatgc acacttgtat tgtacaggca gccgcgagtc cacccgatga
     3301 acgcaacctt tcccacctat ggcgcggttg atgcgcgcaa acttctggcc gtggccggcc
     3361 ccgtgcatgc cggtccggca cggcggacag cgcgatgatc ggcaccgacg tgctcatcat
     3421 cggcggcggc atcgccggcg cctcgctcgg ctacgcgctg gcgcgcccgc aggatgccgt
     3481 gctgctcgaa gccgaggacc agccgggcta tcactccacc ggccggtcgg cggcgctgta
     3541 catgaccacc tacggccatc cgctgatccg cgcgctgacg cacgccagcc gggcgttcct
     3601 cgagcgcccg ccggcgggct tcgccgaggt ggcgctgatg cagctgcgcg gcgtgctgtg
     3661 gattgcgcgg gctgatcagc aggcggcgtt tgcccgctac tacgccgatt gcagcgcgct
     3721 ggaccgctcg gtcgcgcggt gcacggtgga gcaggcgctg gcgctgtgcc ccgcgctgca
     3781 gtctgcatac gtcaaccagg cgttcgtcga acccagcgcg caggacatgg acgtgtcggc
     3841 g
//
LOCUS       DASZZC010000186         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_270793,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000186 DASZZC010000000
VERSION     DASZZC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270793"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VHE37_04080"
     CDS             complement(<1..1558)
                     /locus_tag="VHE37_04080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT34730.1"
                     /translation="MTGLLIAVLMLSLGAVAWLGRGQWARLRRAQAEHHLYDGGAFAL
                     LVLDAGGKICAANDAALRLLGAGREALLREDLLRFHDMQELAVRANALSHELSTMVMP
                     GFDVLIAKARLGTTDENDWTLVRRDGSRVPAHLAISALRDAAGAVSGYMVCAYDIGER
                     RRREQYIQHIAQHDGLTHLPNRMLLQDRLQVALVQARQHGEQVGVLMVDLDHFKRIND
                     SLGHHTGDQLLVTVAERLRGCVRDGDTVARMGGDEFAIVLADVKNSGDVQQVAQSVVD
                     TLSRPMRVGAHELQVTPSVGVSRFPEDGGDVGTLLKHADTALYRAKAEGRSRLRMFSA
                     DLLHVATEKLELEGALRQALARGELVMHYEPQVSLASGQLIGMEALVRWQHPERGLIS
                     PAVFIPVAEESGLIVQLGEWTLRTACRETRQLQERSGRPLTVSVNLSPRQFGQARLVD
                     MVRSALAESGLAPQHLVLEVTEGVLMQHADETIALLAELRALGVSIAVDDFGTGYSSL
                     SYISRFPVNVL"
     gene            1710..2264
                     /locus_tag="VHE37_04085"
     CDS             1710..2264
                     /locus_tag="VHE37_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HVT34731.1"
                     /translation="MRVAAAVLLLMLAGCAAAPLRLAPAEAVAKVDLDRYMGTWYVIG
                     RTPSYNERRQVAVTVDYARGQDGSIFIVQHSRDQDFTHAPEQLSGRGSVVDPVSNALW
                     SVKFPWSGAQDYAVVYLDDSYQHAIAVDAPHQRVWLLAREARVSDDTYEVYLAVLRAK
                     NFDTDKVVRVPQIEDQLGAPGYTP"
     gene            complement(2252..2983)
                     /gene="bioD"
                     /locus_tag="VHE37_04090"
     CDS             complement(2252..2983)
                     /gene="bioD"
                     /locus_tag="VHE37_04090"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HVT34732.1"
                     /translation="MSPATARTLFVTGTDTSVGKTHAACALLARARREGVRACGYKPV
                     AAGGVRDNGQVRNEDVLALQHAAGTREPYAAINTYTFERAIAPHLAARAEHARVQVTR
                     LHDAHAQLAQRHELVVVEGAGGWQVPLNDDLTFAGWVAQQGWPVLLVVGMRLGCLNHA
                     LLSVESIARRTRLLGWIANVLPPEQEALADNIETLRALVPAPLYGVLPARGGIEAAAD
                     ALDFSALWTALTRRDEIEDDEPQGV"
     gene            complement(3071..>3981)
                     /locus_tag="VHE37_04095"
     CDS             complement(3071..>3981)
                     /locus_tag="VHE37_04095"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HVT34733.1"
                     /translation="ELAQFCGRERALLFSTGWAANLGVLRALLGKADTLIADELNHAS
                     LIDGGKLSGARYVRVAHADADAYARELAQAQGLRLVATDSVFSMDGDIAPLEELSALC
                     RERDAALMVDDAHGFGVTGARGRGACVAPPDIYVATLGKSIGCAGAFVAGDEALIEYL
                     IQRARSWIFSTAPPPALAAAARRALAIVNSDEGEELRARLHARVGEFRDGCAQRGIAL
                     GVGGIGSASAIQPLVLGEETRALALSKFLYERGLWVAAIRPPTVPRGTARLRITLSAA
                     HGPAEVQRLLDLLAEGVKMLPPHPAS"
BASE COUNT          588 a         1447 c         1340 g          606 t
ORIGIN      
        1 tgagcacgtt gaccgggaag cgcgagatgt acgacaggct ggagtagccg gtgccgaaat
       61 cgtcgaccgc gatcgacacg cccagcgcgc gcagctcggc cagcagcgcg atggtctcgt
      121 ccgcgtgctg catcagcacg ccctcggtca cttccagcac caggtgctgc ggcgccaggc
      181 ccgactcggc cagcgccgag cgcaccatgt cgaccagccg ggcctgcccg aactggcgtg
      241 gcgaaagatt gaccgacacc gtcagcggcc gaccgctgcg ctcctgcagc tgccgggtct
      301 cgcggcaggc ggtgcgcagg gtccactcgc ccagctgcac gatcaggccg gattcctccg
      361 ccaccgggat gaacaccgcc ggcgagatca ggccgcgctc ggggtgctgc cagcgcacca
      421 gcgcttccat gccgatgagc tggccgctgg ccagcgacac ctgcggctcg taatgcatca
      481 ccagctcgcc gcgcgccagc gcctggcgca gcgcgccttc cagctcgagc ttctcggtgg
      541 ccacgtgcag caagtcggcg gagaacatgc gcaggcggct gcgcccttcc gccttggccc
      601 ggtacagcgc agtgtcggca tgcttgagca gcgtgcccac gtcgccgccg tcctcgggaa
      661 agcggctgac gccgaccgac ggcgtcacct gcagttcgtg cgcgcccacg cgcatcggcc
      721 gcgacagcgt atcgaccacg ctctgcgcca cctgctggac gtcgccgctg ttcttgacat
      781 cggccagcac gatggcgaat tcgtcgccgc ccatgcgcgc caccgtgtcg ccatcgcgca
      841 cgcagccgcg caggcgctcg gccaccgtca ccagcagctg gtcgccggta tggtggccca
      901 gcgaatcgtt gatgcgcttg aagtggtcga gatcgaccat cagcacgccc acctgctcgc
      961 cgtgctggcg cgcctgcacc agcgccacct gcaggcggtc ctgcagcagc atgcggttgg
     1021 gcaggtgggt gaggccgtcg tgctgcgcga tgtgctggat gtactgctcg cgccggcggc
     1081 gttcgccgat gtcgtacgcg cagaccatgt agccgctgac cgcgccggcc gcatcgcgca
     1141 gcgcgctgat ggccaggtgc gcgggcacgc ggctgccgtc gcgccgcacc agcgtccagt
     1201 cgttctcgtc ggtggtgccc agccgcgcct tggcgatgag cacgtcgaag cccggcatga
     1261 ccatggtcga cagctcgtgc gacagcgcat tggcgcgcac cgccagttcc tgcatgtcgt
     1321 ggaagcgcag caggtcttcg cgcagcagcg cctcgcgccc ggccccgagc agccgcagcg
     1381 cagcgtcgtt ggcggcgcag atctttccgc cggcgtcgag caccagcagg gcgaaggcgc
     1441 cgccgtcgta aagatggtgc tcggcctgcg cccggcgcag gcgcgcccac tggccgcggc
     1501 ccagccaggc cacggcgccc agcgacagca tcagcacggc gatgagcaat ccggtcatcc
     1561 tggcctcgtg gaatttcctg gatcctattg tccttctgac agccgcatca ggttcatcgc
     1621 cgggcgttat tcgccagcgg cttgtggata gcttcgccgc agtgccgcta ctatcgcagc
     1681 cgcgtgtacc aggaatccgg ggaactccga tgagagtcgc tgctgcggtg ctgctgttga
     1741 tgctggccgg ctgcgccgcc gccccgctgc gcctggcgcc ggcggaggcg gtggccaagg
     1801 tcgacctcga ccgctacatg ggcacctggt acgtcatcgg ccgcacgccg agctacaacg
     1861 agcggcgcca ggtggcggtc accgtcgatt acgcgcgcgg ccaggacggt tcgatcttca
     1921 tcgtgcagca ttcacgcgac caggatttca cgcatgcgcc ggagcagctg tccggccgcg
     1981 gcagcgtggt cgatccggtc agcaacgcgc tgtggtcagt caagttcccg tggagcggtg
     2041 cgcaggacta cgccgtcgtc tacctcgacg acagctacca gcacgccatc gccgtggatg
     2101 cgccgcacca gcgcgtgtgg ctgctggcgc gcgaggcgcg cgtgagcgac gacacctacg
     2161 aggtctatct cgccgtgctg cgcgcgaaga atttcgacac cgacaaggtg gtgcgcgtgc
     2221 cgcagatcga agaccagctc ggcgcgcccg gttacacccc ctgaggctcg tcgtcttcga
     2281 tctcgtcgcg gcgcgtaagc gccgtccata gcgcggaaaa atccagcgcg tcggccgcgg
     2341 cctcgatgcc gccgcgcgcc ggcagcacgc cgtacagcgg cgccgggacc agcgcacgca
     2401 gcgtctcgat gttgtcggcc agcgcctcct gctccggcgg cagcacgttg gcgatccagc
     2461 cgagcaggcg cgtgcgccgc gcgatgctct ccactgacag cagcgcgtgg ttcaggcagc
     2521 ccagccgcat gccgaccacc agcagtaccg gccagccctg ctgcgccacc cagccggcga
     2581 acgtcaggtc gtcattcagc ggcacctgcc agccgccggc gccttccacc accaccagct
     2641 cgtggcgctg cgccagttgc gcatgcgcgt catgcaggcg cgtgacctgg acgcgcgcgt
     2701 gttccgcgcg cgcagccaga tgcggcgcga tggcgcgctc gaaggtgtac gtgttgatcg
     2761 ccgcgtaggg ctcgcgcgtg ccggccgcgt gctgcagcgc cagcacgtct tcgttgcgca
     2821 cctgtccgtt gtcgcgcacg ccgcccgcgg cgacgggctt gtagccgcag gcgcgcacgc
     2881 cttcgcgccg cgcgcgcgcg agcagcgcgc aggccgcgtg cgtcttgccc acgctggtgt
     2941 cggtgccggt gacgaacagc gtgcgggccg ttgcggggct catcgagcgc cagaattcgc
     3001 ctgaccgcac tgcccttttt gatgttctat attattgtat aattttattt tcatattata
     3061 attaataaca tcacgaagcg ggatgcggtg gcagcatttt cacaccctcc gcgagcaggt
     3121 cgagcagccg ctgcacctcg gccgggccgt gcgcggccga cagcgtgatg cgcaggcgcg
     3181 cggtgccgcg cggcaccgtc ggcgggcgga tcgctgccac ccacaggcca cgctcgtaaa
     3241 ggaacttcga caaggccagg gcgcgcgtct cctcgcccag caccagcggc tggatggcgc
     3301 tcgcgctccc gatgccgccg acgccgagcg cgatgccgcg ttgcgcgcag ccgtcgcgga
     3361 actcgcccac gcgcgcgtgc agccgcgcgc gcaattcctc gccttcgtcc gagttcacaa
     3421 tcgccagcgc gcggcgcgcg gccgcggcca gcgccggcgg cggcgcggtg gagaagatcc
     3481 agctgcgtgc gcgctggatg agatattcga tcagcgcttc atctccggcc acgaacgcgc
     3541 cggcgcagcc gatcgatttg cccagcgtcg ccacgtagat gtccggcggc gcgacgcagg
     3601 cgccgcggcc gcgcgcgccc gtcacgccga agccatgcgc gtcgtcgacc atcaacgcgg
     3661 cgtcgcgctc gcggcacagc gccgacaatt cttcgagcgg tgcgatatcg ccgtccatgg
     3721 agaacacgct gtcggttgcg accagccgca agccctgcgc ctgcgccagc tcgcgcgcgt
     3781 acgcatccgc atcggcgtgc gccacgcgca cgtagcgcgc gcccgacaat tttccgccgt
     3841 cgatcagcga cgcgtggttg agttcgtcgg cgatcagcgt gtcggccttg cccagcagcg
     3901 cgcgcagcac gccgaggttg gcggcccagc cggtggagaa cagcagcgcg cgctcacgcc
     3961 cgcagaactg cgccagctct t
//
LOCUS       DASZZC010000187         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_270873,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000187 DASZZC010000000
VERSION     DASZZC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270873"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..791
                     /gene="kdsB"
                     /locus_tag="VHE37_04100"
     CDS             36..791
                     /gene="kdsB"
                     /locus_tag="VHE37_04100"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HVT34734.1"
                     /translation="MKFKVVIPARLGSTRLPRKVLREIAGKPLVQHVWNAARASGADE
                     VIVATDDAEVLAACKVFGADARMTSVAHQSGTDRANEIARAAGWSADTLVVNLQGDEP
                     LMPPALIGQAARLLAEDAGADIATLCHPIHELEEWLNPNVVKCVLDARAYALYFSRAP
                     IPWQRDGATREAPKLPAKLAYRHIGLYAYRVRALQEFSALPPALLEQCEALEQLRALH
                     AGLRIKLAVVQMPPPRGVDTEEDLLAVAKLLRG"
     gene            complement(909..1301)
                     /locus_tag="VHE37_04105"
     CDS             complement(909..1301)
                     /locus_tag="VHE37_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVT34735.1"
                     /translation="MPAVLIEVRRSYTREQQAALIDAVHGGLRDALRLPEWDRTVRLV
                     THDAACFAASPRLKQPENFTLITINLFAGRSLEAKRALYRGIRERLEALGIARDHVNI
                     VLVESERENWGLRGVPGSEIELGFKVEV"
     gene            complement(1301..2632)
                     /locus_tag="VHE37_04110"
     CDS             complement(1301..2632)
                     /locus_tag="VHE37_04110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVT34736.1"
                     /translation="MDVTIFGSGYVGLVTAACFADAGNQVLCVDIDARRVETLSRGDV
                     PFYEPGLAELVKANFKAGRLKFTTDGAAGVAHGQFQFIAVGTPPGEDGSADLKYVLEV
                     ARTIGKHMTEHRVLVTKSTVPVGTCDKVRKAAQAELDARGAKIEFDVVSNPEFLKEGA
                     AIADFTKPDRIVIGSDHARAATLMRQLYAPFNVNHDRIIVMDVKSSELTKYAANAMLA
                     TKISFMNELANVAERVGADIEQVRIGIGSDPRIGYHFIYAGCGYGGSCFPKDVKALVH
                     TARDAGHHAPLLEAVETVNERQKHVLFRRMREHYGDLKGKTIAIWGLAFKPRTDDMRE
                     ATSRVLMEELWAAGATVRAFDPVANHETEKIYGKRADLVLCSTAEEAAHDADALAIVT
                     EWKEFRSPNFARIAKSLREPVIFDGRNLYEPEFMKNMGYTYYSIGRQSVSA"
BASE COUNT          433 a          920 c          936 g          495 t
ORIGIN      
        1 tcgcgcgcaa ggcgcgctcc tgcagacagc cgcacatgaa attcaaggtc gtcatccccg
       61 cgcgcctggg ctccacgcgg ctgccgcgca aggtgctgcg cgagatcgcc ggcaagccgc
      121 tggtgcagca cgtgtggaac gctgcgcgcg ccagcggcgc cgacgaggtg atcgtcgcca
      181 ccgacgacgc cgaggtgctg gccgcgtgca aggtgttcgg tgccgatgcg cggatgacct
      241 cggtggcgca ccagtccggc accgaccgcg ccaacgagat cgcgcgcgcc gccggctgga
      301 gcgccgacac gctggtggtg aacctgcagg gcgacgagcc gctgatgccg ccggcgctga
      361 tcggccaggc tgcgcgcctg ctggcggaag atgccggggc ggatatcgcc acgctgtgcc
      421 atccgatcca cgagctggag gagtggctta atcccaacgt ggtcaagtgc gtgctcgatg
      481 cgcgcgccta cgcgctgtat ttctcgcgcg cgccgatccc gtggcagcgc gacggcgcca
      541 cgcgagaggc gccgaagctg ccggccaagc tggcctatcg ccacatcggc ctgtatgcct
      601 accgggtgcg cgcgctgcag gaattttctg cgctgccgcc ggcgctgctc gaacaatgcg
      661 aagcgctgga gcagctgcgc gcgctgcatg ccggtttgcg catcaagctc gcggtggtgc
      721 agatgccgcc gccgcgcggc gtggacaccg aggaagatct gctggcggtg gcgaagctgc
      781 tgcgcgggtg atttttcact ccaccgcacg gccggcttcg atgttatttt ttattgtctt
      841 aaagtaaaaa cagacaatat tttataaccg cgacaggcaa ggtgcggtga agaaaaaaat
      901 cgccgtgttc agacctcgac cttgaatccg agctcgatct cggagccggg cacgccgcgc
      961 aggccccagt tctcgcgctc cgattccacc agcacgatgt tgacgtggtc gcgcgcgatg
     1021 cccagcgctt cgagtctttc cctgatgccg cgatacagcg cgcgcttggc ctcgagcgag
     1081 cggccggcga aaagattgat ggtgatgagc gtgaaatttt cgggctgctt gagccgcggc
     1141 gatgccgcga agcatgcggc gtcatgcgtc accagccgca cggtgcggtc ccactcgggc
     1201 agcctgagcg cgtcgcgcag gccgccgtgc accgcgtcga tcagcgcagc ctgctgttcg
     1261 cgcgtatagc tgcgccgcac ctcgatcagc accgccggca tcaggcgctt acgctctggc
     1321 ggccgatgga gtaatacgtg tagcccatgt ttttcatgaa ctcgggctcg tagagattgc
     1381 gcccgtcgaa gatcaccggc tcgcgcaggc tcttggcgat gcgcgcgaag ttggggctgc
     1441 ggaattcctt ccactcggtg acgatggcca gcgcgtcggc gtcgtgcgcc gcttcctcgg
     1501 cagtgctgca cagcacgagg tcggcgcgct tgccgtagat cttctcggtt tcgtggttgg
     1561 ccaccgggtc gaacgcgcgc accgtggcgc cggcggccca cagctcctcc atcagaaccc
     1621 tgctcgtggc ttcgcgcatg tcgtcggtgc gcggcttgaa cgccaggccc cagatggcga
     1681 tggtcttgcc cttgaggtcg ccgtagtgct cgcgcatgcg ccggaacagc acgtgcttct
     1741 gccgctcgtt gaccgtttcc accgcctcca gcagcggcgc gtggtgcccg gcgtcgcgcg
     1801 cggtgtgcac cagcgccttc acgtccttgg ggaagcacga gccgccgtag ccgcagccgg
     1861 cgtagatgaa gtggtagccg atgcgcggat cggagccgat gccgatgcgc acctgttcga
     1921 tgtcggcgcc gacgcgctcg gccacgttgg ccagctcgtt catgaagctg atcttggtcg
     1981 ccagcatggc gttggcggcg tacttggtca gctcgctgga cttcacgtcc atcacgatga
     2041 tgcggtcgtg gttgacgttg aacggcgcgt acagctggcg catcagcgtg gcggcgcgcg
     2101 cgtggtcgct gccgatgacg atgcggtcgg gcttggtgaa atcggcaatg gccgcgcctt
     2161 ccttgaggaa ctcgggattg gacaccacgt cgaactcgat cttcgcgccg cgcgcgtcga
     2221 gctccgcctg cgccgccttg cgcaccttgt cgcaggtgcc gaccggcacg gtggacttgg
     2281 tcaccagcac gcggtgctcg gtcatgtgct tgccgatggt gcgcgccact tccagcacgt
     2341 acttgagatc ggccgagccg tcttcgcccg gcggcgtgcc caccgcgatg aactggaact
     2401 gcccgtgcgc cacgccggcg gcgccatcgg tggtgaactt caggcgcccg gccttgaagt
     2461 tggccttgac cagctcggcc aggcccggct cgtagaacgg cacgtcgccg cgcgacagcg
     2521 tttccacgcg ccgcgcgtcg atgtccacgc acaacacctg gttgcccgca tcggcaaaac
     2581 aggcggcggt gaccaggccg acgtagccgg aaccgaagat ggtgacgtcc atatttatcg
     2641 ccccaaaaaa agtagcctcc cgccgcgcgt ggcggacggg aggcgttatt caatcacatc
     2701 gaccgctcag gccggacggt cgcccgaggc gtgctcgccg gaaggctgcg ccgccgcctt
     2761 gctgtcctcg aacatcttga tgta
//
LOCUS       DASZZC010000188         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_529243,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000188 DASZZC010000000
VERSION     DASZZC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529243"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VHE37_04115"
     CDS             <1..225
                     /locus_tag="VHE37_04115"
                     /inference="COORDINATES: protein motif:HMM:NF018829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HVT34737.1"
                     /translation="SQVFTPEAFEGVGIDLAGKKIIALKSSQHFHAGYAPIASEIIYV
                     ATPGSTAQDFANIPYRKRSLDYWPRVEKPA"
     gene            complement(256..1314)
                     /locus_tag="VHE37_04120"
     CDS             complement(256..1314)
                     /locus_tag="VHE37_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT34738.1"
                     /translation="MELQSALDLESFWQACLKLVTTRLPHRSCSLMFNIVDFEPTDAR
                     HHVARSKNPNYMPATSLTVSSPFLARHPQIKLYTYSQIVSEDPDARRRRIEQEPDPEW
                     NEFIHLAFWHDRGPEAVFSIHWPPDRENISGEERAFLEQLHPMIEAGLRRLRKLEVER
                     ARRQVYEHFLQQVPLSVMFVDGDGEMLFATPEAERQCARWNRALRSAVPAELQSRLPQ
                     QVTRMYDAAPGSKMQAPPAAASVRLAHPSLENLAIKIDRSWHFPGLQLRPCYVITFLD
                     SAAAGQPAELSQAAMLVLQQLSPSERRVAQLVARGLRNEQIAEHLCRSRRTIEFQLNS
                     VYRKLGISSRTQLARALT"
     gene            complement(1442..>2290)
                     /locus_tag="VHE37_04125"
     CDS             complement(1442..>2290)
                     /locus_tag="VHE37_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HVT34739.1"
                     /translation="WTPADAAAAGARSLVIGVAPKGGQFADNWIPVLRAAVDAGLDIV
                     SGMHSKLAEVPGLAAAAQARGVRLVDVRTPPAGLPTGSGRKRSGMRLLTVGTDCGLGK
                     KYTALALAHALQARGVDADFRATGQTGIMIAGSGIPLDAVVADFIAGAAECLSPDAAP
                     EHWDIVEGQGSLFHPAYAGVTLGLIHGSQPDALVLCHDPQRTHVNSLRDFALPSLDLA
                     AARNLEAARLTNPAARLVGVSLITENMGDEQRARVLGAAEKLLGVPAFDPLRTSLQPV
                     LDRLLP"
BASE COUNT          354 a          784 c          796 g          356 t
ORIGIN      
        1 tcgcaggtgt tcacgcccga ggcgttcgaa ggcgtcggca tcgatctcgc gggcaagaaa
       61 atcatcgcgc tgaaatcgtc gcagcatttc cacgccggtt acgcgccgat tgccagcgag
      121 atcatctacg tggccacgcc cggctcaacc gcgcaggatt tcgccaacat tccttaccgc
      181 aagcgcagcc tggactactg gccgcgggtg gaaaagcccg cttgatgctt ccggcggcgc
      241 cggccgccgc gcgcttcagg taagcgcgcg cgccagctgc gtgcgcgagc tgatgcccag
      301 cttgcgatag accgagttga gctggaactc gatggtgcgg cgcgagcggc acaggtgctc
      361 ggcaatctgc tcattgcgca aaccgcgcgc caccagctgt gccacgcggc gctcgctggg
      421 cgagagctgc tgcagcacca gcatcgccgc ctgcgacagt tccgccggct gcccggccgc
      481 ggcgctgtcg aggaaggtga tgacatagca cggccgcagc tgcaggccgg ggaaatgcca
      541 gctgcggtcg atcttgatcg ccaggttctc cagcgacgga tgcgccagcc tgacgctggc
      601 tgccgcgggc ggtgcctgca tcttgctgcc gggggccgcg tcgtacatgc gcgtgacctg
      661 ctgcggcagc cgcgactgca gttccgccgg caccgcgctg cgcagcgcgc ggttccagcg
      721 cgcgcactgg cgttccgcct cgggcgtggc gaacagcatc tcgccgtcgc cgtcgacgaa
      781 catcaccgac agcggcacct gctgcaggaa gtgctcgtag acctggcgcc gcgcgcgttc
      841 cacttccagc ttgcgcagtc ggcgcaggcc ggcctcgatc atcgggtgca gctgctcgag
      901 aaacgcgcgc tcttcgccgc tgatgttctc gcggtcgggc ggccagtgga tgctgaacac
      961 cgcttcgggt ccgcggtcgt gccagaacgc caggtggatg aactcgttcc actccgggtc
     1021 cggctcctgc tcgatacggc ggcggcgcgc atccgggtct tccgacacga tctgcgaata
     1081 ggtgtacagc ttgatctgcg gatgccgcgc caggaacggg ctggacaccg tcaggctggt
     1141 ggccggcatg tagttgggat tcttcgaacg cgccacgtgg tgccgcgcgt cggtgggctc
     1201 gaagtcgacg atgttgaaca tcaggctgca cgagcggtgc ggcaaccgcg tggtgaccag
     1261 cttgaggcag gcctgccaga aactttccag gtccagcgcg ctctgcagtt ccacgattgc
     1321 ttcgcgcaaa tccgtggaat attgcgggta cagggtgcgc gccgggtgca tggcaaaaag
     1381 cagaacaaaa gccctgctgc cggccggcgc gctaggtgga aatacctagc ccgcgcggcc
     1441 gctatggcag cagccggtcc agcaccggct gcagagaagt ccgcaacgga tcgaacgccg
     1501 gcacgccgag cagtttttcc gccgcgccga gcacacgcgc acgctgctca tcgcccatgt
     1561 tttcggtgat caggctcacg ccaaccaacc gtgccgcagg attggtcagg cgcgcggctt
     1621 caaggttgcg cgccgccgca aggtccagcg atggcagcgc gaagtcgcgc agcgagttga
     1681 cgtgcgtgcg ctgcgggtcg tggcacagca ccagcgcgtc gggctgcgag ccgtggatca
     1741 ggccgagcgt gacgccggcg taggccgggt ggaacagcga gccctggcct tcgacgatgt
     1801 cccagtgctc cggcgcggca tcgggcgaca ggcattccgc cgcgccggcg atgaagtcgg
     1861 cgaccaccgc gtcgagcggg atgccgctgc ccgcgatcat gatcccggtc tggccggtgg
     1921 cgcggaaatc cgcatcaacg ccgcgcgcct gcagcgcatg cgccagcgcc agcgcggtgt
     1981 atttcttgcc caggccgcaa tcggtgccga cggtgagcag gcgcatgccg ctgcgcttgc
     2041 gcccgctgcc ggtgggcagg cccgcgggcg gcgtgcgcac gtcgaccagg cgcacgccgc
     2101 gcgcctgcgc cgccgccgcg aggcccggca cttcggcgag cttgctgtgc atgccgctga
     2161 cgatgtcgag gccggcgtcc accgccgcgc gcagcacagg gatccagttg tccgcgaact
     2221 gcccgccctt gggcgcgacg ccgatcacca gcgagcgtgc gccggcagcg gccgcgtctg
     2281 ccggcgtcca
//
LOCUS       DASZZC010000189         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_395351,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000189 DASZZC010000000
VERSION     DASZZC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395351"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VHE37_04130"
     CDS             <1..1310
                     /locus_tag="VHE37_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT34740.1"
                     /translation="GMVAVEFEVYTRTGDKRVWAFSASCPGRLQDGRRFAVGMAVDIT
                     DRKEAEQALKQADKRKDEFLATLAHELRNPLAPIRNALSILRMDAPASDAAAQVHEMM
                     ERQLNYLVRLVDDLFEVSRITRGNIELRLERAELGALIRSAVETSRPLIDAAQHSLTV
                     ELPPGPMPVEADPVRLVQVFANLLNNAAKYTPDGGRIRVTARADDSHVIVAVQDNGNG
                     IAPEMQPRVFDMFTQLESPALYKRGGLGLGLAIVRGLVRMHGGTVDVRSDGPGKGSEF
                     LVCLPLAAAQAAPQPVAVPAEPAGDKGQRIMIVDDNVDAADSLGILLRMHGAHVHVAH
                     AAQDALDVLAGFDPDVIFMDLGMPGMDGFEAARRIRANPRFARVALIALSGWGAENDR
                     RRSRAAGFDHHLIKPADFTALQNLLASLPRRGEDKGKVVQLFG"
     gene            1465..>1922
                     /locus_tag="VHE37_04135"
     CDS             1465..>1922
                     /locus_tag="VHE37_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HVT34741.1"
                     /translation="MSAAPAARAALPTPAELEPALDRALSHARTRGAAQAEASVSVSQ
                     GLTVSVRKGEVESVEFQRDRELGITVYFGQRKGNATTGDLSPAAIESAVDAACAIAQA
                     TGEDPCNGLAEAALMARDFPDLDLDHPWALSPDQAVDLARECEAAALAVDA"
BASE COUNT          315 a          657 c          675 g          275 t
ORIGIN      
        1 agggcatggt ggcggtggaa ttcgaggtgt acacgcgcac cggcgacaag cgcgtgtggg
       61 ccttctccgc ctcgtgtccc ggccgcctgc aggacggccg ccgcttcgcc gtcggcatgg
      121 cggtggatat caccgaccgc aaggaagccg agcaggcgct gaagcaggcg gacaagcgca
      181 aggacgaatt cctggccacg ctggcgcacg agctgcgcaa cccgctggcg ccgatccgca
      241 acgcgctgtc catcctgcgc atggacgcgc cggccagcga cgccgcggcg caggtacacg
      301 agatgatgga gcgccagctc aactacctgg tgcgcctggt cgacgatctg ttcgaggtgt
      361 cgcgcattac ccgcggcaac atcgagctgc gcctggagcg cgcggaactg ggcgccctga
      421 tccgcagcgc ggtggagacc agccgcccgc tgatcgacgc cgcgcagcac tcgctcacgg
      481 tggaactgcc gcccgggccc atgccggtgg aggccgaccc ggtgcggctg gtgcaggtat
      541 tcgccaacct gctcaacaac gccgccaagt acacgcccga cggcggccgc atccgcgtga
      601 ccgcgcgcgc cgacgacagc cacgtgatcg tcgccgtgca ggacaacggc aacggcattg
      661 cgccggagat gcagccgcgc gtgttcgaca tgttcaccca gctggagtcg ccggcgctgt
      721 acaagcgcgg cgggctgggg ctgggcctgg ccatcgtgcg cgggctggtg cgcatgcacg
      781 gcggcaccgt cgacgtgcgc agcgacgggc cgggcaaggg gagcgaattc ctggtgtgcc
      841 tgccgctggc cgcagcgcag gcggcgccgc agccggtggc ggtgccggcc gagccggccg
      901 gcgacaaggg ccagcgcatc atgatcgtcg acgacaacgt cgatgccgcc gacagcctgg
      961 gcatcctgct gcgcatgcac ggcgcgcacg tgcacgtggc gcatgccgcg caggatgcgc
     1021 tcgacgtgct ggcgggcttc gatcccgacg tgatcttcat ggacctgggc atgccgggca
     1081 tggatggctt cgaggcggcg cggcgcatcc gcgccaatcc gcgctttgcc cgcgtggcgc
     1141 tgattgcgct gtccggctgg ggtgcggaaa acgaccgccg gcgctcgcgc gcggccggtt
     1201 tcgaccatca cctgatcaag ccggcggact tcaccgcgct gcagaacctg ctggcctcgc
     1261 tgccgcgccg cggtgaggac aagggcaagg tggtgcagct gttcggctga tttttgccgc
     1321 caccgcaccc ctgccggtcg tgttataaaa aattgtatat atataaaaat ataatataat
     1381 taataacact gaaactgccg gtgcggtgga gtccaaaaag acgcgctaag ctgccctccc
     1441 cgcgtatcac caccaaaaaa atccatgtcc gccgcacctg ccgcccgcgc cgcactgccg
     1501 acgcctgctg aactcgaacc cgcgctcgac cgcgcgctgt cgcatgcgcg tacgcgcggc
     1561 gcggcgcagg ccgaggccag cgtgtcggtg agccagggcc tgacggtgag cgtgcgcaag
     1621 ggtgaagtgg agagcgtgga attccagcgc gaccgcgagc tcggcatcac cgtgtacttc
     1681 ggccagcgca agggcaacgc caccaccggc gacctgtcgc cggcggcgat cgagtccgcg
     1741 gtcgacgccg cctgcgccat tgcccaggcc accggcgagg atccgtgcaa tggcctggcc
     1801 gaggccgcgc tgatggcgcg cgactttccc gacctggacc tggatcatcc gtgggcactg
     1861 tcgcccgacc aggcggtcga cctggcgcgc gagtgcgagg cggcggcgct ggcggtggac
     1921 gc
//
LOCUS       DASZZC010000190         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_978217,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000190 DASZZC010000000
VERSION     DASZZC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978217"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VHE37_04140"
     CDS             complement(<1..1192)
                     /locus_tag="VHE37_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVT34742.1"
                     /translation="MIQNLRNIAIIAHVDHGKTTLVDKLLRQSGTLDKREELGERIMD
                     SNDLERERGITILAKNTAIRWTDSRSGTEYRINIVDTPGHADFGGEVERVLSMVDCVC
                     LLVDAADGPMPQTRFVTQKAFNMGLNPIVVVNKIDRPGARAHWVIDQVFDLFDRLGAT
                     DQQLDFPIVYASALHGYAGDNPDIRDGNMDFLFQTIVDKVPVPDVHPEEPFQMQVTSL
                     AYSSYVGVIGTGRITRGKVKTNQPVAVVARDGKVRQGRVLQVLGFMGLDKIEVPEAEA
                     GDIIAVTGIEELGISETICDVKTPEGLPTLQVDEPTMSMTFEVNKSPFAGKEGKFVTS
                     RQIGERLQRELKTNVALRVEDTDSADKFKVSGRGLLHLGILLENMRREGYEVAVSRPE
                     VIEKE"
     gene            1346..3358
                     /locus_tag="VHE37_04145"
     CDS             1346..3358
                     /locus_tag="VHE37_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVT34743.1"
                     /translation="MHNARPRRPWNAVINDKVRQFLFGKKLDPLNPSTRGHIALVAFL
                     AWIGLGADGLSSACYGPEEAFLALGVHMHLALYLALATAGTVFVISLAYNQVIELFPS
                     GGGGYKAATKLVGPNAGLVSGSALIVDYVLTIAISAASGVDALFSLLPVGALHWKLPA
                     EFFVVVALILLNLRGMKESIYVLMPVFLGFFISHLLLIVYGIGAHADRIGGLLPQTIA
                     ETQHLSGQMGWVFVVSLFLKAYSVGAGTYTGIEAVSNNVNSLAEPRVRTGHWTMFYMA
                     TSLAFTAGGIILLYLLWQAHPEDGKTLNAVVFAAIIEHAGWHSPTLSHGALAATMLLE
                     AGLLMVASNTGFLGGPAVMASMAADRWVPRQFRQLSDRMVTQNGILVMGVAALAILLI
                     TRGAVAVLVVLYSINVFLTFTLSLYGLTRYWWTQRLYALHWRGKFLLSGFGLAVTSSI
                     LLVILTEKFTQGGWVTLVITGVVIGICWLIRGHYDETARAMKRIDQLYELKNTWGDDT
                     QPLPVDPAARTAVFLVGGSRGAGMHALQWVLKTFAGQFQNFVFVSVGEVDKQSFDTQR
                     NIQHLQTRIDNSLHYFTSYCASRGLAATSFRAFGADPLDELTHLLTTQVLKDFPNAVC
                     FASKLIFRNENALTRVLHNQMPLSVQQRLQLVGYELIIVPIELPQT"
     gene            complement(3462..>4425)
                     /gene="purT"
                     /locus_tag="VHE37_04150"
     CDS             complement(3462..>4425)
                     /gene="purT"
                     /locus_tag="VHE37_04150"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HVT34744.1"
                     /translation="LIVPEIEAIHTPTLQALEQEGYTVIPTALAARLTMDREGIRRLA
                     AEELRLPTSPYRFAGNEADYRAAVQAVGLPCVVKPIMSSSGKGQSMVKTAADIQHAWD
                     YAQAGGRAGAGKVIVEGFVDFDYEITMLTVRHAGPGGAVTTSFCDPIGHLQIDGDYRE
                     SWQPHPMSAAALQKSQDIARAITGNLGGRGVFGVELFIKGDQVIFSEVSPRPHDTGLV
                     TLISQDLPQFSLHARAILGLPIPNIRQRGPAASCAILCEGDRKAPSFGGLDEALAEPD
                     TMLRLFGKPEIVGKRRMGVALALATDIEAARAKARRAAAAVKLA"
BASE COUNT          674 a         1469 c         1462 g          820 t
ORIGIN      
        1 cttccttctc gatcacttcc gggcgcgaca ccgcgacctc gtagccttcg cggcgcatgt
       61 tctcgagcag gattccaaga tgcagcaggc cgcggcccga caccttgaac ttgtcggcgc
      121 tgtccgtgtc ctccacgcgc agcgccacgt tggtcttgag ctcgcgctgc aggcgctcgc
      181 cgatctggcg gctggtgacg aacttgcctt ccttgccggc gaacgggctc ttgttgacct
      241 cgaaggtcat gctcatggtc ggctcgtcga cctgcagcgt gggcaggcct tccggcgtct
      301 tcacgtcgca gatggtttcc gagatgccca gttcctcgat gccggtgacg gcgatgatgt
      361 cgccggcttc cgcttcgggg acttcgatct tgtccaggcc catgaagccc agcacctgca
      421 gcacgcggcc ctggcgcacc ttgccgtcgc gcgccaccac cgccaccggc tggttggtct
      481 tgaccttgcc gcgggtgatg cggccggtgc cgatgacgcc gacataagag gagtaggcca
      541 gcgaggtcac ctgcatctgg aacggctctt ccgggtggac gtcgggcacc ggcaccttgt
      601 cgacgatggt ctggaacagg aagtccatgt tgccgtcgcg gatgtccggg ttgtcgccgg
      661 cgtaaccgtg cagcgcgctg gcgtagacga tggggaaatc cagctgctgg tcggtggcgc
      721 cgagccggtc gaacaggtcg aacacctggt cgatgaccca gtgcgcgcgc gcgcccgggc
      781 ggtcgatctt gttcaccacc acgatcgggt tgaggcccat gttgaaggcc ttctgcgtga
      841 cgaagcgcgt ctgcggcatg gggccgtcgg cggcgtccac cagcaggcat acgcagtcga
      901 ccatcgacag cacgcgctcc acttcgccgc cgaagtcggc gtggcccggg gtgtcgacga
      961 tgttgatgcg gtactcggtg ccgctgcggc tgtcggtcca gcggatcgcg gtgttcttgg
     1021 ccaggatggt gatgccgcgt tcacgctcca ggtcgttgga gtccatgatg cgttcgccca
     1081 gttcctcgcg cttgtccagc gtgccggact ggcgcagcag cttgtcgacc agcgtggtct
     1141 tgccatggtc gacgtgggcg atgatggcga tgttgcgaag attctgaatc atgaaagaca
     1201 ccaaaaatat ttgagggcag cgtagccgcc tcgtttgaca atccggcgca tgcggcagcc
     1261 ggcaatgggg acagggatgc cgaaagggcc gcggattgta cgtgaagccg cccccggaag
     1321 cagccgattt ttccaccctg cgcaggtgca taatgcgcgc ccccggcgac cctggaacgc
     1381 ggtcatcaac gacaaagtcc gccagtttct cttcggcaag aagctcgatc ccctgaatcc
     1441 cagcacgcgc ggccacatcg cgctggtggc gttcctggcg tggattggcc tcggtgccga
     1501 cggcctgtcg tcggcctgct atggccccga ggaggcgttc ctggcgctgg gcgtgcacat
     1561 gcacctggcg ctgtacctgg cgctggctac cgccggcacc gtgttcgtca tctcgctggc
     1621 ctacaaccag gtgatcgaac tgttcccctc gggcggcggc ggctacaagg cggccaccaa
     1681 gctggtaggg cccaatgccg gcctggtgtc gggctcggcg ctgatcgtcg actacgtgct
     1741 gaccatcgcc atctcggccg ccagcggcgt cgacgcgctg ttcagcctgc tgccagtggg
     1801 cgcgctgcac tggaagctgc cggcggaatt cttcgtggtg gtggcgctga tcctgctcaa
     1861 cctgcgcggc atgaaggaat cgatctacgt gctgatgccg gtattcctcg gcttctttat
     1921 cagccatctg ctgttgatcg tctacggcat cggcgcgcat gccgaccgca tcggcggatt
     1981 gctgccacag accatcgccg agacccagca cctgtcaggg cagatgggct gggtgttcgt
     2041 ggtctcgctg ttcctcaagg cttactcggt gggcgccggc acctacactg gcatcgaggc
     2101 ggtatcgaac aacgtcaact cgctggccga gccgcgcgtg cgcaccggcc actggaccat
     2161 gttctacatg gccacctcgc tggccttcac cgccggcggc atcatcctgc tgtacctgct
     2221 gtggcaggcc cacccggagg atggcaagac gctcaacgcg gtggtgttcg ccgccatcat
     2281 cgagcatgcc ggctggcaca gcccgacgct gagccacggc gccctggccg cgaccatgct
     2341 gctggaagcc gggctgctga tggtggcgtc caacaccggt ttcctgggtg gtcccgccgt
     2401 gatggcgagc atggccgcgg accgctgggt gccgcgccag ttccgccagc tgtccgatcg
     2461 catggtcacg cagaacggca tcctggtgat gggcgtcgcg gcgctggcca tcctcctgat
     2521 cacccgcggc gcggtggcgg tgctggtggt gctgtactcc atcaacgtgt ttctcacctt
     2581 cacgctgagc ctgtacggcc tgacgcgcta ctggtggacg caacggctgt atgcactgca
     2641 ctggcgcggc aagttcctgc tctcgggctt cggcctggcg gtcaccagca gcatcctgct
     2701 ggtgatcctc accgagaaat tcacccaggg cggctgggtc acgctggtca tcaccggcgt
     2761 ggtcatcggc atctgctggc tgatccgcgg ccactacgac gagaccgcgc gcgcgatgaa
     2821 gcgcatcgac cagctttacg aattgaaaaa cacctggggc gatgacacgc agccgctacc
     2881 agtcgatccc gcggcgcgaa cggcggtttt cctggtcggc ggcagccgcg gcgcgggcat
     2941 gcacgcgctg caatgggtgc tgaaaacctt tgccggccag ttccagaact tcgtgttcgt
     3001 ctcggtgggc gaggtggaca agcagagctt cgacacccag cgcaacatcc agcacctgca
     3061 gacgcgcatc gacaactcgc tgcactactt caccagctac tgcgccagcc gcggcctcgc
     3121 ggcaacttcc ttccgcgcct tcggcgccga tccgctggat gagctcaccc acctgctgac
     3181 cacgcaggtg ctgaaggatt ttcccaacgc ggtctgcttc gccagcaagc tgatcttccg
     3241 caacgagaac gcgctcaccc gcgtgctgca caaccagatg ccgctgtcgg tgcagcagcg
     3301 cctgcagctg gtgggttacg aactgatcat cgtgccgatc gagctgccgc agacctgaat
     3361 aaccagtttt tgccgcgctc gcgcggcggg aaataacgcc tcggcaggtg attgtctggt
     3421 tgaccgtggt ggctgtaagc ctgcgcagcc gctgcgcagg cttacgcgag cttcaccgcc
     3481 gccgccgcgc gccgcgcctt ggcccgcgct gcttcgatat ccgtagcaag ggccagcgcc
     3541 acgcccatgc gccgcttgcc gacgatctcc ggcttgccga acaggcgcag catggtgtcg
     3601 ggctcggcca gcgcttcgtc caggccgccg aacgacggcg ccttgcggtc gccctcacag
     3661 aggattgcgc atgacgctgc cggcccgcgc tggcggatgt tggggatggg caggccgagg
     3721 atggcgcgcg catgcagcga gaactgcggc aggtcctggc tgatcagcgt caccagcccg
     3781 gtgtcgtgcg ggcgcggcga cacctcgctg aagatcacct ggtcgccctt gatgaacagc
     3841 tccacgccga acacgccgcg gccgccgagg ttgccggtga tggcgcgcgc aatgtcctgc
     3901 gatttctgca gcgcggcggc gctcatcggg tgcggctgcc acgactcgcg gtagtcgccg
     3961 tcgatctgca ggtggccgat ggggtcacag aacgacgtgg tcaccgcgcc gccggggccg
     4021 gcatgccgca cggtgagcat ggtgatctcg taatcgaaat cgacgaagcc ttcgacgatg
     4081 accttgcccg ccccggcgcg gccgccggcc tgggcgtagt cccaggcgtg ctggatgtcg
     4141 gcggcggtct tcaccatgct ctggcccttg cccgagctgg acatgatcgg tttgaccacg
     4201 cagggcagcc cgaccgcctg cactgccgcg cggtagtccg cctcgttgcc ggcaaaacga
     4261 tagggcgagg tcggcagcct gagctcttcc gccgccagcc gccggatgcc ctcgcggtcc
     4321 atggtcaggc gcgccgccag ggcggtgggg atgacggtgt agccttcctg ctccagggcc
     4381 tgcagggtcg gggtgtggat cgcctcgatc tccggcacga tcagg
//
LOCUS       DASZZC010000191         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1041030,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000191 DASZZC010000000
VERSION     DASZZC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041030"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHE37_04155"
     CDS             complement(<1..392)
                     /locus_tag="VHE37_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT34745.1"
                     /translation="MSFNTTGFKTLLKKEVMRFWNVLGQTVTAPAITAILYLLVFAQA
                     LQGRASAYEGVSYTQFLLPGLVMMAIIQNAFANTSSSMIQSKVMGNLVFLLMAPLGEL
                     EWFAAYIGAALVRAALVGGAMLIVCLPFV"
     gene            complement(505..1416)
                     /locus_tag="VHE37_04160"
     CDS             complement(505..1416)
                     /locus_tag="VHE37_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT34746.1"
                     /translation="METAIAINGVKKNYGKVQALKGVSFEIGRGELFGLLGPNGAGKS
                     TLINITAGLVRMNAGSIAVLGHDTVSSFREARRNLGVVPQELVFDPFFNVVDMLRIQA
                     GYFGCGKESWPWIDQLIERMDLTAKRNASMRSLSGGMKRRVLIAQALVHKPPVVILDE
                     PTAGVDVELRRTLWSFVRELHAGGHTIVLTTHYLEEAEDLCQRIAIIDKGEVKVIEKT
                     RDLLSRHPFRFLKLKLNGKGLPDSLRALVVSEKDGEIELKLDRKQHPVGNVLDALRDA
                     GARVEDIHTREPTLEDIFVEMTKGAPA"
     gene            1474..>2211
                     /locus_tag="VHE37_04165"
     CDS             1474..>2211
                     /locus_tag="VHE37_04165"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HVT34747.1"
                     /translation="MSTALVWFRRDLRLSDNPALAAACERHARVVPVYIHAPDEDAPW
                     TPGAATRWWLQHSLAALDRSLRGQLVIRRGPSLPALRTLARECGAQAIYWNRLYEPVS
                     VARDTRIKTALRAENLDVESFNGALWLEPQQLKNGSGEPYRVFTPFWKALQQRLPPRA
                     LLPEPRTLKTPATLPGSLRLEALELLPRIRWDAQFFAHWTPGEDGAHARLNRFCEEIL
                     DGYSGTRDLPSRDGTSRLSPHLHFGEIS"
BASE COUNT          364 a          768 c          712 g          367 t
ORIGIN      
        1 acgaaaggca ggcagacgat gagcatggcg ccgccgacca gcgccgcgcg caccagcgcg
       61 gcgccgatgt aggcggcgaa ccattccagt tcgcccagcg gcgccatcag caggaagacg
      121 agattgccca tcaccttgga ctggatcatc gacgaactgg tgttggcgaa ggcgttctgg
      181 atgatcgcca tcatcaccag gcccggcagc aggaactgcg tgtagctcac gccctcgtac
      241 gccgaggcgc ggccctgcag cgcctgcgcg aacaccagca ggtaaagaat cgcggtgatg
      301 gccggcgcgg tgacggtctg gccgagcacg ttccagaacc gcatgacctc tttcttgagc
      361 agcgtcttga aaccggtggt gttgaagctc atgcggattc catcgccccg gcgtaggccg
      421 gggcccagtt ttgattttcg gtcgatcgag gagaaaaacc ggattccggc ctgcgccgga
      481 atgacaagcc aaagcaacgc ccgttcatgc cggcgccccc ttggtcatct ccacgaagat
      541 gtcctccagc gtcggctcgc gcgtgtggat gtcctccacc cgcgcgccgg catcgcgcag
      601 cgcgtcgagc acgttgccca ccgggtgctg cttgcggtcg agcttgagtt cgatctcgcc
      661 gtccttctca ctcaccacca gcgcgcgcag cgagtcgggc agacccttgc cgttgagttt
      721 caatttcagg aagcgaaacg ggtggcgcga cagcaggtcg cgcgtcttct cgatcacctt
      781 cacctcgccc ttgtcgatga tcgcgatgcg ctggcacagg tcctcggctt cctcgaggta
      841 atgcgtggtc agcacgatgg tgtggccgcc cgcgtgcagc tcgcgcacga acgaccacag
      901 cgtgcggcgc agttccacgt cgacgccggc ggtgggctcg tcgaggatca ccaccggcgg
      961 cttgtgcacc agcgcctgcg cgatcagcac gcgccgcttc atgccgcccg acagcgagcg
     1021 catcgaggca ttgcgcttgg ccgtcaggtc catgcgctcg atcagctggt cgatccacgg
     1081 ccagctttcc ttgccgcagc cgaagtagcc ggcctggatg cgcagcatgt cgaccacgtt
     1141 gaagaacggg tcgaacacca gttcctgtgg caccacgccc aggttgcgcc gcgcctcgcg
     1201 gaagctgctc accgtgtcgt gcccgagcac cgcgatcgaa ccggcgttca tgcgcaccag
     1261 cccggcggta atgttgatca gcgtggactt gccggcgcca ttgggaccga gcaggccgaa
     1321 caactcgccg cggccgatct cgaaggagac gcccttcagc gcctgcacct tcccgtaatt
     1381 cttcttgacg ccgttgatgg cgatggcagt ttccatgggg cgcgaattat acctgccgca
     1441 tccatgcgcc cggttcatcc gtagcatgcg cgcatgagca ctgccctcgt ctggttccgc
     1501 cgcgacctgc gcctgtccga caaccccgcg ctggccgccg cctgcgagcg gcacgcccgc
     1561 gtcgtgccgg tgtacatcca tgcgccggac gaggacgcgc cgtggacccc gggcgcggcc
     1621 acgcgctggt ggctgcagca cagcctggcc gcgctggacc ggtcactgcg cgggcagctg
     1681 gtgatccgcc gcggcccgtc gctgccggcc ttgcgcacgc tcgcgcgcga atgcggcgcg
     1741 caggcgatct actggaaccg gctgtacgag ccggtgagcg ttgcgcgcga cacgcgcatc
     1801 aagactgcgc tgcgcgcaga aaacctggac gtggagagct ttaacggcgc gctgtggctg
     1861 gagccgcagc agctgaagaa cggcagcggc gaaccgtacc gcgtattcac tccgttctgg
     1921 aaagcgctgc agcagcgcct gccgccgcgc gcgctgctgc cggagccgcg caccctcaag
     1981 acgccggcaa cgctcccggg ctcgctcaga ctggaagcgc tcgagctgct gccgcgcatc
     2041 cgctgggacg cgcagttttt cgcgcactgg acgccgggcg aagacggcgc acacgcgcgg
     2101 ctcaaccgct tctgcgagga aatcctcgat ggctactccg gcacgcgcga cctgccctcg
     2161 cgtgatggca cttcgcgcct gtcgccgcat ctgcatttcg gcgagatcag c
//
LOCUS       DASZZC010000192         6432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_466655,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000192 DASZZC010000000
VERSION     DASZZC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466655"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VHE37_04170"
     CDS             <1..1198
                     /locus_tag="VHE37_04170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT34748.1"
                     /translation="EMGVSERRRSTGETAHEPAAAARLRQQYYDAISRQIEHETPGLI
                     RRRIAQAVAMAGVLAFILVDYFSDGAARSGWAAQLDWKLIATVLPLIVLPIAYAFPQQ
                     RRMALGQYALMGMFVIALALAGAMDIGRGQRAWLNFESLMLVTIYIYFISGLPLFRAI
                     FCGLCAWLAFTVARVETPGVSLAAREIYYLLAANVVGTIGLGYMERSARERFGDQFEL
                     RVTAMIDSLTGVLNRGAVRSHIERTWRQAQREDKTYAMLLADLDQFKSINDVHGHPVG
                     DACLMHVADVLSKLARRPLDAVGRWGGDEFVAVWYDLDARHFSTMLKDLPAAIAQFKP
                     APNVSTMEPLSLTGGAVLVLPGGQHDFSEVMEQADALLYKAKRSGRGQILTALAVDLD
                     AGNKAA"
     gene            1305..3953
                     /locus_tag="VHE37_04175"
     CDS             1305..3953
                     /locus_tag="VHE37_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT34749.1"
                     /translation="MKALLLAVAALCLPLSALAQDAAPATEPSASEPPAPTDTASAGQ
                     PAEAAAPAEAEPAPPPATETVAEPAAAAAEAPAAPEAPEQAAPQSAGEVGTIAVQGTE
                     PAAAPALPLEQAPNRIDEVVVTAQKTKQSARKVPLSITALDGAQMKQVGAASLADVSL
                     YVPNVRVDAHDPGSPQVFIRGFGTNAFNPSFESSVAFVQDDLYFGRPGYFTESMFDIE
                     RIEVLRGPQGTLFGKNTVAGVFNVISKGPEGDPSVDGSVYYGNHNERRVEGGAGGMLT
                     DWLGARIAGFFLDKDGQLYNDFLNRYEDKLRQEAGRLKLKLAPGNGFESELTAAHSRT
                     NAPFWPFELMNLDSDTTTYLRGFDPKVEGNPYDFTTSFDTQGWIDKGSDTVSVRTHWD
                     HGSDFGAIHDFDPVLVLGWSRFHIDQLNELDVSPADIARLDSHERHRQLSAELRFTGR
                     TDSLLGLGTGVQFVGGAFWFKSRYDLFARVLAGQDLPSYLLTKDACQLLAASNGLDPS
                     VCGPLGILALPGLSALGSLTSAVTNNDYYQFNYGQDIDSTALFGQFTWNITDKIAVTP
                     GLRLSRENKGVDANGQSHCQTKDSGLGGPCVMEQVISSSDYHEPSLDKSEFDASPKLA
                     LQYFADHGVNYYTSYAKGYKSGGFNAISLKGGNLQYEPEKAQTWELGAKARFFDNTLS
                     WNLTLYQTHFDNLQVLAFNGLFFDVSNAGTARSRGLETDFLWLTPYAPLRIAGSGGLL
                     NAIYLKYDGAPAPIEDGIGQKQSLAGQRIAFAPRSSATLTPTLTYPFFGDLMLTFSGD
                     VIWQGNQFVDTNLDPKKYVGGYTKFAARMVLAHPDGRWSISLGGNNLSDKRVLNQVVD
                     ATFFPGSYFAQQAAGRTVFGMVTFKL"
     gene            complement(3961..4404)
                     /locus_tag="VHE37_04180"
     CDS             complement(3961..4404)
                     /locus_tag="VHE37_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34750.1"
                     /translation="MNDTRTQKPFDILLVEDSASDALMAREALAAGGLPHALHVVSDG
                     IEAIAFLRREGGYADAPRPSLIYLDLNMPRKSGREVLAEIKADPDLRDIPVVVLTTSS
                     AEKDINEAYRLHANAYMIKALDFDQFVNAITCAQRFWFDVVALPG"
     gene            4793..>6432
                     /locus_tag="VHE37_04185"
     CDS             4793..>6432
                     /locus_tag="VHE37_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HVT34751.1"
                     /translation="MRVRPSMSHDEMVAALTRGRWDPLRVDGSIARSWDRSAGHSLDP
                     ETTNAPQVLPAHRLREYRAPLEPLLHVARGAMETLHQQVRDAGYVILLTNPEGITVDF
                     MNNLPLDRELRRAGLYLGGCWPEGVEGTNAIGLCVVERRPVTVHQSEHFRKVNATLTC
                     SSVPLRSETGELLAVLDASALYSPDEKRSQLLILQMVVAAAAQIENAYFLQRFERNWI
                     LRISRRRELLEVAADGLIALDDGGRILAANAPICQALGEEQASMVGRAIDDVLHMPFA
                     RIADAESKSSVLHWPHLAGGLYGVARRPIRLVLPASLRSQRRTDAPSLAALAGGDPQM
                     QACVERARLVLDKGIAILLQGETGTGKELFARAVHQASSRAAAPFVALNCAAIPETLI
                     ESELFGYRSGAFTGALAKGAQGKLVLAHGGTLFLDEIGDMPPALQTRLLRVLSEREVT
                     PLGTDKAVPVDLQVICATHRDLVQLVQSGAFREDLYYRINGISLQLPPLRERADRPEL
                     IEALLAEESAALGRASCSLSDEAGALLVGYSWPGNVRQ"
BASE COUNT         1058 a         2173 c         2153 g         1048 t
ORIGIN      
        1 cgagatgggt gtatcagaac gcaggcgaag taccggggaa acggcgcatg aaccggccgc
       61 cgccgcgcgc ctgcgccagc agtactacga cgccatcagc cggcagattg aacatgaaac
      121 gccagggctg atccgccgcc gcatcgcgca ggcggtggcc atggccggtg tgctggcctt
      181 catcctggtc gactattttt ccgacggagc cgcgcgcagt ggctgggcgg cgcagctgga
      241 ctggaaactg atcgccaccg tgctgccgct gatcgtgctg cccatcgcct atgcctttcc
      301 gcagcagcgc cgcatggcgc tgggccagta tgcgctgatg ggcatgttcg tcatcgcgct
      361 ggcactggcc ggcgccatgg acatcggccg cggccagcgc gcctggctca acttcgaatc
      421 gctgatgctg gtcaccatct acatctactt catttccggc ctgccgctgt tccgcgccat
      481 cttctgcggc ttgtgcgcgt ggctggcgtt caccgtggcg cgggtggaga cgcccggcgt
      541 gtcgctggcc gccagagaaa tctattacct gctggcggcg aacgtggtcg gcaccatcgg
      601 cctgggctac atggagcgca gcgcgcgcga gcgcttcggc gaccagttcg aactgcgcgt
      661 caccgccatg atcgattcgc tcaccggcgt gctcaaccgc ggcgccgtgc gctcgcacat
      721 cgagcgcacc tggcgccagg cgcagcgcga ggacaagacc tacgccatgc tgctggccga
      781 cctggaccag ttcaagagca tcaacgacgt ccacggccac ccggtgggcg acgcctgcct
      841 gatgcacgtg gccgacgtgc tgtcaaagct ggcgcggcgc ccgctcgacg cggtgggccg
      901 ctggggcggc gacgagttcg tggcggtgtg gtacgacctc gacgcgcggc acttctccac
      961 catgctgaag gacctgcccg cagccatcgc gcagttcaag ccggcgccga acgtgtccac
     1021 catggaaccg ctgtcgctca ccggtggtgc ggtgctggtg ctgccgggcg gccagcatga
     1081 tttttccgag gtgatggagc aggccgacgc cctgctgtac aaggccaagc gcagcgggcg
     1141 cgggcagatc ctcaccgcgc tggcggtgga tctcgacgcc ggcaacaagg cggcctgacc
     1201 tttcccgcgc cgggtcccgg cgcgtttcct gcgtgcaccc ggcggggcgc ggatgccaca
     1261 atggcgccgc gtcgtggaat caccgcaggg atcaccagaa aaccatgaaa gcgcttttgc
     1321 tcgccgttgc cgccttgtgc ctgccgttgt ccgcgctggc gcaggatgcc gcgccagcca
     1381 ccgaaccctc cgccagcgaa ccgcccgcgc cgacagacac tgcatccgcg ggtcagccgg
     1441 cagaagcggc agcgcccgcg gaagctgaac cggcgccgcc gcctgctacg gaaaccgtag
     1501 cagagccagc cgccgccgcc gccgaagcgc cggctgcgcc cgaggcgccc gagcaggccg
     1561 cgccacaatc ggccggtgaa gtcgggacca tcgccgtgca gggcaccgag ccggccgccg
     1621 cgccggcgct gccgctggag caggcgccca accgcatcga tgaagtggtg gtgaccgcgc
     1681 agaagaccaa gcagtcggcg cgcaaggtgc cgctgtcgat caccgcgctc gacggcgcgc
     1741 agatgaagca ggtgggcgcg gccagcctgg ccgacgtctc gctctatgtg cccaacgtgc
     1801 gcgtcgatgc gcacgacccc ggctcgccgc aggtgttcat ccgcggcttc ggcaccaacg
     1861 ccttcaaccc gagcttcgaa agctccgtcg cgttcgtgca ggacgacctg tatttcggcc
     1921 gccccggcta tttcaccgag tcgatgttcg acatcgagcg catcgaagtg ctgcgcgggc
     1981 cgcagggcac gctgttcggc aagaacaccg tggccggcgt gttcaacgtg atcagcaagg
     2041 ggcccgaggg cgatccctcg gtcgacggct cggtctacta cggcaaccac aacgagcggc
     2101 gcgtggaagg cggcgccggc ggcatgctca ccgactggct cggcgcgcgc atcgccggct
     2161 tcttcctcga caaggacggc cagctgtaca acgacttcct caaccgttac gaggacaagc
     2221 tcaggcagga agccggccgc ctcaagctca agctggcgcc cggcaacggc ttcgaatccg
     2281 agctcaccgc ggcgcattcg cgcaccaacg cgccgttctg gccgttcgag ctgatgaacc
     2341 tggacagcga caccaccacc tacctgcgcg gcttcgatcc gaaggtcgag ggcaacccct
     2401 acgatttcac cacctcgttc gacacgcagg gctggatcga caagggcagc gacacggtga
     2461 gcgtgcgcac gcactgggat catggcagcg acttcggcgc catccacgac ttcgacccgg
     2521 tgctggtgct gggctggtca cgcttccaca tcgaccagct caacgagctc gacgtgtcgc
     2581 cggccgacat cgcgcggctg gacagccacg agcggcaccg ccagctgtcg gcggaactgc
     2641 gcttcaccgg ccgcaccgac tcgctgctcg gcctgggcac cggggtgcag ttcgtcgggg
     2701 gcgcgttctg gttcaagtcg cgctacgacc tgtttgcgcg cgtgctcgcc gggcaggacc
     2761 tgccgtcgta cctgctgacc aaggacgcgt gccagctgct ggcggcgagc aacgggctcg
     2821 atccctcggt gtgcgggccg ctgggcatcc ttgcgttgcc gggcctgtcc gcgctgggca
     2881 gcctgacctc ggcagtgacc aacaacgatt actaccagtt caattacggc caggacatcg
     2941 actccaccgc gctgttcggc cagttcacct ggaacatcac cgacaagatt gcggtcacgc
     3001 cgggcctgcg cctgtcgcgc gagaacaagg gcgtggatgc caatggccag agccactgcc
     3061 agaccaagga cagcgggctg ggcgggccgt gcgtgatgga gcaggtgatc tcgtcgagcg
     3121 actaccacga accgtcactg gacaagagcg agttcgacgc ctcgcccaag ctcgcgctgc
     3181 agtacttcgc cgaccacggc gtgaactact acacctcgta tgccaagggc tacaagagcg
     3241 gcggcttcaa cgccatctcg ctcaagggtg gcaacctgca gtacgagccg gagaaggcgc
     3301 agacctggga gctgggcgcc aaggcgcgct tcttcgacaa cacgctgtcg tggaatctca
     3361 cgctgtacca gacgcacttc gacaacctgc aggtgctggc cttcaacggc ctgttcttcg
     3421 acgtgagcaa cgcgggcacc gcgcgctcgc gcgggctgga gacggatttc ctctggctca
     3481 cgccgtacgc gccgctgcgc atcgccggct cgggcggttt gctcaacgcc atctacctca
     3541 agtacgacgg cgcgccggcg ccgatcgagg acggcatcgg ccagaagcag agcctggccg
     3601 ggcagcgcat cgcgtttgcg cccaggagca gcgccacgct cacgccgacg ctgacctatc
     3661 cgttcttcgg cgacctgatg ctgacgtttt ccggcgacgt gatctggcag ggcaaccagt
     3721 tcgtcgacac caatctcgat ccgaagaaat acgtgggcgg ctacaccaag ttcgccgcgc
     3781 gcatggtgct ggcgcacccg gacgggcgct ggtcgatatc cctcggcggc aacaacctct
     3841 ccgacaagcg cgtgctcaac caggtggtcg atgccacgtt cttccccggt tcctacttcg
     3901 cgcagcaggc ggcgggtcgc accgtgttcg gcatggtgac gttcaagctg tagcgcacgc
     3961 tcaacccggc aaggcaacca cgtcgaacca gaaccgctgc gcgcaggtga tggcgttgac
     4021 gaactgatcg aagtccagcg ccttgatcat gtaggcgttg gcgtgcagcc ggtacgcttc
     4081 gttgatgtcc ttctcggcgc tcgaggtggt aagtacgacc accggtatat cgcgcaggtc
     4141 cgggtccgcc ttgatctcgg ccagcacttc gcggccgctt ttgcgcggca tgttgagatc
     4201 caggtaaatc agagagggac gcggtgcatc cgcatagccg ccctcgcggc gcaggaaagc
     4261 aatggcttcg atgccgtcgc tgaccacgtg caacgcatgc ggcagcccgc ccgctgccag
     4321 cgcctcgcgc gccatcaggg catcgctggc gctgtcctct accaggagga tgtcgaacgg
     4381 cttctgcgtg cgtgtatcgt tcatgggtga cccttggctt cctgtcgacg cgccacggat
     4441 gtggcgtcgc ggaaactgca gcggccgtgc cagccgcgga gcgcgcggcg acggctcacg
     4501 cggcgccgcg ctgccgaacc cggcaacggc acccggatca agcacatgcg gtagtgcgcg
     4561 caccgtgcgc cggtctgcgg cgccgccggc gccaagacgc ggcgtgcaac acatgggcgc
     4621 gacagctgtc gcaattgcat ctctcgtgca acacctgtcg caggccgccg tgccaggctg
     4681 aacgcgggta cgggcgcatg catcacgcag tgcttgccgt tgcggggtga ggtcctggca
     4741 agcgtatgat ccggctgtca gttgggccgg gccgcgaaca ggagcagatc tgatgcgcgt
     4801 gaggccgtcg atgtcccacg atgaaatggt tgccgcgctg acacgcggcc gctgggaccc
     4861 gttgcgggtt gacggcagca tcgcgcgatc gtgggatcgc tcggccgggc acagtcttga
     4921 ccccgaaacc accaatgctc cgcaggtgtt gccggcacat cgcctgcgcg aatatcgcgc
     4981 gccgctcgag cctctgctgc atgtcgcccg cggtgcgatg gagaccttgc atcagcaggt
     5041 gcgtgatgcc ggctacgtga tcctgctgac caaccctgaa ggcatcacgg tcgatttcat
     5101 gaacaacctg ccgctggacc gggaactgcg cagggcggga ctctacctgg gcggctgctg
     5161 gccggaaggg gtcgagggca ccaatgccat cgggctgtgc gtggtggagc gtcgcccggt
     5221 taccgtccac cagagcgagc atttccgcaa ggtcaacgcc acgctcacct gcagttcggt
     5281 gccgctgcgt tcggagactg gcgaactgct tgcagtcctc gacgcttccg ccctgtattc
     5341 gccggacgaa aagcgcagtc agttgctgat cctgcagatg gtcgttgccg ccgccgccca
     5401 gatcgagaac gcctattttt tgcagcgctt cgagcgcaac tggattctgc gcatcagccg
     5461 gcggcgggaa cttctcgaag tggcggccga cgggctgatc gcgctcgatg acggcggccg
     5521 catcctcgcc gccaatgctc cgatctgcca ggcgctgggc gaggaacagg caagcatggt
     5581 cggccgggcc atcgacgatg tgctgcacat gcccttcgcc cgcattgccg atgcggagtc
     5641 gaaatcttcg gtgctgcatt ggccgcatct ggctggaggg ctgtacggcg tggcgcggcg
     5701 gccgatccgg ctggtgctgc cggcgtcgct ccggtcccag cggcgcaccg acgcgccaag
     5761 cctcgctgcg ctcgccggtg gcgatccgca gatgcaggcg tgcgtcgagc gcgcgcggct
     5821 ggtgctcgac aagggcattg ccatcctgct gcagggggaa accggcacgg gcaaggaact
     5881 gtttgcccgg gccgtgcacc aggcgagcag ccgcgcggca gcgccgtttg tcgcgctcaa
     5941 ttgcgctgcc attccggaaa cgctgatcga gagtgaactg ttcggctatc gcagcggcgc
     6001 tttcaccggc gcgcttgcca agggtgcgca gggcaagctg gtgctggcgc atggaggcac
     6061 cctgttcctg gatgaaatcg gcgacatgcc gccggcgctg cagacgcggc tgctgcgggt
     6121 gctgtcggag cgggaagtga cgccgctggg tacggacaag gcggtgccgg tggacctgca
     6181 ggtgatatgc gccacccacc gcgaccttgt tcagctggtg caaagcgggg cgttccgcga
     6241 ggacctgtac taccgcatca acggcatttc cctgcagctg ccgccgctgc gcgagcgtgc
     6301 cgaccggcca gagctgatcg aagcgctgct ggccgaggaa tccgccgccc tgggccgcgc
     6361 gtcctgcagc ttgagcgatg aggccggggc gctgctggtg ggctactcat ggcccggcaa
     6421 tgtgcgccag tt
//
LOCUS       DASZZC010000193         8202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1235723,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000193 DASZZC010000000
VERSION     DASZZC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235723"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2597
                     /locus_tag="VHE37_04190"
     CDS             <1..2597
                     /locus_tag="VHE37_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVT34752.1"
                     /translation="PLKNGTVEISGDAKHPANAGKLCVKGSALGETVSLDGRLLHPIV
                     RGQRAGWDTALDTVAREFKAVIEEYGPDAVAFYVSGQLLTEDYYVANKLMKGYIGSAN
                     IDTNSRLCMSSAVAGHKRAFGEDLVPVSYDDLEQSDLVLLVGSNTAWCHPVVYQRIAR
                     AKEQRPEMKVVVLDPRRTATCDIADLHLPLKPGSDVWLFNGLLAFLHRHGVEDTAFID
                     AHTAGLARTLVIAENTAGDVRSVARACGVDEAQLGEFFGMFARTEKVITMFSQGVNQS
                     SAGADKVNSIINCHLLTGRIGKPGTGPFSITGQPNAMGGREVGGLANMLAAHMELGNA
                     AHRETVREFWNSPVMADHAGLKAVELFDAMAEGKIKAVWIMATNPVVSLPDADKVKAA
                     LSRCKFVVVSDVIGQTDTNAFAHVLLPAAAWGEKDGTVTNSERQISRQRAFLPLPGEA
                     KPDWWIVSEVGKRMGYADAFAYRNAAQIFAEHAALSAYRNHDERAFNIGAHAQIDTGA
                     YDDLQPFRWGAAQLFADGRFYHQDRRARFVPTPPRAPANPRDDEYPLVLNTGRVRDHW
                     HTMTRTARSPKLSSHIAEPFLDLHPQDALICGVRAGELARVSSRWGSMVARVQTGGGI
                     ARGQVFAPIHWNAQHASDARVGSVVNPVVDPVSGEPEFKHTPVKVEPFGVSWHGFALT
                     RDGGARLPAHDDISYWARITGAQFTRYELAGRGTPRARGTWARALLDATDAQADWLEY
                     EDESAGLYRGAHVEGGRLRGCIYLSPRPDLPSRAWLSSLYAKKNLDDADRAGLLLGAP
                     LASEADHGPTVCSCFGVGRNLILRTAREKKLKTPGEVTACLKAGGNCGSCVPEIRALL
                     AEASTEAA"
     gene            2724..3341
                     /gene="mobA"
                     /locus_tag="VHE37_04195"
     CDS             2724..3341
                     /gene="mobA"
                     /locus_tag="VHE37_04195"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HVT34753.1"
                     /translation="MAAAPFSDDFHGTHARDACTGLVLAGGRGTRFGGADKGLVEFRG
                     RPLAQHALDLLRPQVRSCLVSANRNRESYERFGAPVLADEHSGFPGPLAGMATGLAHC
                     PTRWLLCVPCDMPQLPAALAESLLRSALLKHRPAAYAVFDGDPAYVCALLHRDLLDPL
                     RAFLAGGQRAVRAWLAACNACAVDFPDAVGAVNLNSALDLREALG"
     gene            3370..4335
                     /gene="moaA"
                     /locus_tag="VHE37_04200"
     CDS             3370..4335
                     /gene="moaA"
                     /locus_tag="VHE37_04200"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HVT34754.1"
                     /translation="MVDRHGRRKRKLRLSLTDRCNFRCRYCMPQQPQWMKKKDLLTRD
                     ELLALAHLFVGSGIEAIRLTGGEPLLRADLVECVATINTLRAHGLRRISLTTNGSHLA
                     GRLAALRAAGLDDLNISLDALDPARFAALRGADIKPVLEGIGAALNQRVPFKLNTVLV
                     RGHNDCEILPLVDWAMARGAPLRFIEYMPLDAPGTWSAEQVVSAAQILRTLRTRHRVE
                     PLLRGSEPASEYLLDGEFRVGIISTISNPFCASCDRVRLTARGELYTCLFAPHGTPLG
                     AQLRAGADATILRNAIHSAVWNKQAGYAANPQPVERPLLMHAMGG"
     gene            4341..4583
                     /locus_tag="VHE37_04205"
     CDS             4341..4583
                     /locus_tag="VHE37_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HVT34755.1"
                     /translation="MNVTFELLGVLQRLCGSERIQLQVHAATVADALEQLALARPDLR
                     GELERCAIASGDAIVRRRDALSPGAVLALLPPVAGG"
     gene            4588..5052
                     /locus_tag="VHE37_04210"
     CDS             4588..5052
                     /locus_tag="VHE37_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HVT34756.1"
                     /translation="MSPHLTELPLQLPSLLAEGEYPQCGGLAVFGGAVRNHHEGKPVL
                     RLRYTAYRPLAERLMREIEQHARRHYGLPHCRVQHRIGLLNIGDVAIYCVTRAPHRAE
                     AFDACRWVVDEVKHRVPIWKEEFYADGSSAFVQGCCIRADRETHPAHDHVHA"
     gene            5049..5990
                     /gene="moaCB"
                     /locus_tag="VHE37_04215"
     CDS             5049..5990
                     /gene="moaCB"
                     /locus_tag="VHE37_04215"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HVT34757.1"
                     /translation="MKDVSLKPDSLRNARASATLHAPAACIELLRKGETEKGDALKTA
                     RVAGILAAKRTDELIPLCHPLPIHKADVTYQLKETSVEVIAEVETIGPTGVEMEALTA
                     ASLAALTLYDMLKPYAPASELRISDTHLLQKKGGKSHYRRTLARPASAAVIVLSDTVA
                     AGRKPDTAGEAVREALAEAGFAPIAYEILPDEPAQLLQSVQRKLADGVQLIAAVGGTG
                     AGPRDLTVDTLRPLVTRDMPGVMEAARAFGQRRTPYSMLSRGIAGFAGDSLLITFPGS
                     LRGARESLAAVLPGLVHLFDVQRAQAVHAGGYSREDN"
     gene            5991..6728
                     /locus_tag="VHE37_04220"
     CDS             5991..6728
                     /locus_tag="VHE37_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT34758.1"
                     /translation="MDPASNLPLLTLGIFIIALLYSAVGHAGASGYIAVMTLFGLSAA
                     VIKPTALVLNIIVASITTLQFARAGHFSWCTFWPFALFAMPLAFAGGYLNLPAHVFKL
                     IVGVVLLASALRFFINPAEPDEVTPPSKPLAFAVGGGLGLLSGLTGTGGGIFLTPLML
                     IMRWAHTRTTAAVSALFILCNSISGLAGNFSSTRNLPSLALPLAVAVVVAGSLGSWLG
                     SRKLPVQAIRRLLAVVLLIAGGKLILT"
     gene            6739..7953
                     /gene="glp"
                     /locus_tag="VHE37_04225"
     CDS             6739..7953
                     /gene="glp"
                     /locus_tag="VHE37_04225"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HVT34759.1"
                     /translation="MMPLDQALATYTRELRALAPRTLPLEQALGHVLAAAVESRVDLP
                     LFTQSAVDGYAVRSADLAGASADRPVTLELAGEIAAGSSAQHELTAGRTFRIFTGGAL
                     PAGADASARQEIVERSGNRIVLRRALAAGADTRFRGEELKAGEVLAQPGQRLGSGLIA
                     ALAMAGVSAVPVHPRPRVALLVSGDEVASSGSELRAGEIHDANGPLVRAWLAERGYPL
                     VWARQLRDDRAGLRAALAEALACADAVITTGGVSVGDHDHLPRAAADAGVRELYWKVA
                     QKPGKPIWFGTGIAGQPVLSLPGNPAAVLIGLHLHVSSALRALEGSTQVEPSWRKARL
                     ARAVKRDEQRDQLLRVCMACGNDGSTTLTPLGRQDSHMLSNLGSADGMAWIRRGTGQL
                     EAGDVVEWLALG"
     gene            complement(7969..>8202)
                     /locus_tag="VHE37_04230"
     CDS             complement(7969..>8202)
                     /locus_tag="VHE37_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HVT34760.1"
                     /translation="SIQSFISGDIKDRYQMIDVNVYQEFIFHTKMRLKELDLDTYLFG
                     EGVSELPPREAKKIRENVNHEIMEIYYGNNIQR"
BASE COUNT         1261 a         2868 c         2807 g         1266 t
ORIGIN      
        1 gcccgctgaa aaacggcacg gtggaaatca gcggagatgc caaacatccg gcgaacgccg
       61 gcaagctctg cgtgaaaggt tcggcgctgg gtgagacggt cagcctcgac ggccgcctgc
      121 tgcatccgat cgtgcgcggc cagcgtgccg gctgggacac tgcgctcgac accgtcgcac
      181 gggaattcaa ggccgtcatc gaggagtacg ggcccgacgc cgtcgccttc tacgtctccg
      241 gccagttgct gaccgaggat tactacgtcg ccaacaagct gatgaagggc tacatcggca
      301 gcgccaacat cgacaccaac tcgcgcctgt gcatgtcgtc ggcggtcgcg ggacacaagc
      361 gcgccttcgg tgaagacctg gtgccggtca gctacgacga ccttgaacaa tccgacctgg
      421 tgctgctggt cggctcaaac accgcctggt gccaccccgt cgtctaccag cgcatcgcca
      481 gggcgaagga gcagcggccg gagatgaagg tggtggtgct cgatccgcgc cgcaccgcca
      541 cctgcgacat cgccgacctg cacctgccgc tcaagcccgg cagcgatgtg tggctgttca
      601 acggcctgct cgccttcctg caccggcatg gcgtggaaga caccgccttc atcgacgcgc
      661 ataccgccgg cctcgcgcgc acgctggtga tcgccgagaa caccgcgggc gacgtgcgct
      721 cggtggcgcg cgcctgcggc gtggatgagg cgcagctcgg cgaattcttc ggcatgttcg
      781 cgcgcacgga aaaagtcatc acgatgtttt cgcagggcgt gaaccagtcc tcggccggcg
      841 ccgacaaggt caacagcatc atcaactgcc acctgctcac cgggcgcatc ggcaagccgg
      901 gcacggggcc gttctccatc accggccagc ccaacgccat gggcgggcgc gaggtcggcg
      961 ggctggccaa catgctggcc gcgcacatgg aactgggcaa cgccgcgcac cgcgagacgg
     1021 tgcgcgaatt ctggaattcg ccggtgatgg ccgatcacgc cgggctcaag gccgtcgagc
     1081 tgttcgacgc catggccgaa ggaaaaatca aggccgtgtg gatcatggcc accaatccgg
     1141 tggtctcgct gcccgacgcg gacaaggtga aagctgcgct gtcgcgctgc aaattcgtcg
     1201 tggtgtccga cgtgatcggg cagaccgaca cgaatgcgtt cgcgcacgta ctgctgccgg
     1261 ccgcggcctg gggcgagaag gacggcaccg tcaccaattc cgagcggcag atctcgcgcc
     1321 agcgagcgtt tcttcccttg ccgggcgagg cgaagcccga ctggtggatc gtttctgaag
     1381 tgggcaagcg catgggatat gccgatgcgt tcgcctaccg gaacgcggcg cagatttttg
     1441 cggagcatgc ggcgctgtcg gcatatcgca accatgacga gcgcgctttc aacatcggcg
     1501 cgcatgcaca gatcgacacc ggcgcgtatg acgatctgca gccattccgc tggggcgcgg
     1561 cgcagctgtt cgccgatggc cgcttctacc atcaggaccg ccgcgcgcgc ttcgtgccca
     1621 cgccgccgcg cgcgccggcc aacccgcgcg acgacgagta cccgctggtg ctcaacaccg
     1681 gccgcgtgcg cgaccactgg cacaccatga cgcgcaccgc acgctcgccg aaactgtctt
     1741 cgcacatcgc cgaaccgttc ctcgacctgc atccgcagga cgcgctgatc tgcggcgtgc
     1801 gcgccggcga actggcgcgc gtgtcctcgc gctggggcag catggtggcg cgcgtacaga
     1861 ccggcggcgg tatcgcgcgc ggccaggtgt ttgcgccgat ccactggaac gcacagcacg
     1921 cctccgatgc gcgcgtgggc agcgtggtga atccggtggt cgacccggtg tcgggcgagc
     1981 cggagttcaa gcacacaccg gtgaaggtgg aaccgttcgg cgtcagctgg catggctttg
     2041 cgctcacccg cgacggcggc gcgcggctgc cggcgcatga cgacatcagc tactgggcgc
     2101 gcatcaccgg cgcgcagttc acgcgctacg aactggccgg gcgcggcacg ccgcgcgcgc
     2161 gcggcacctg ggcgcgcgcg ctgctcgacg ccaccgatgc gcaggccgac tggctggaat
     2221 acgaggacga aagcgccggc ctgtaccgcg gtgcgcatgt cgagggcggt cgcctgcgcg
     2281 ggtgcatcta cctgtcgccg cggcccgacc tgccttcgcg cgcgtggctg tcgtcgctct
     2341 atgccaagaa gaatctcgac gacgccgatc gcgccggcct gctgctcggc gccccgctgg
     2401 caagcgaggc cgaccatggg cccaccgtgt gctcgtgctt cggcgtgggc cgcaacctga
     2461 tcctgcgcac cgcgcgcgag aagaaactga agacgccggg cgaagtcacc gcctgcctca
     2521 aggccggagg caactgcggc tcgtgcgtgc cggagatccg cgcgctgctg gcggaagcgt
     2581 ccaccgaggc cgcatgaacc cgcgttcgca tccaggacgg ccgttttttg cttccaccgc
     2641 accctcattt gttatgttat aaattaatga tcaactttaa atatattgta ttttttataa
     2701 catcgtcagg cggggtgcgg tgaatggctg cagcaccttt cagcgatgat tttcacggca
     2761 cccacgcgcg tgacgcctgc accgggctgg tcctggccgg cggccgcggt acgcgcttcg
     2821 gcggtgccga caaggggctg gtcgaattcc gcggacgccc gctggcgcag cacgcgctcg
     2881 acctgctgcg accgcaggtg cgctcctgcc tggtcagcgc caaccgcaac cgcgaaagtt
     2941 acgagcgctt cggcgcgccg gtgctcgccg acgagcacag cggttttccc gggccgctcg
     3001 ccggcatggc cacggggctg gcgcactgcc ccacccgctg gctgctgtgc gtgccctgcg
     3061 acatgccgca gctgccggcg gcgctggccg aatcgctgct gcgctcggcg ctgctcaagc
     3121 accggcccgc ggcttacgcg gtgttcgacg gcgacccggc ctacgtgtgc gcgctgctgc
     3181 accgcgacct gctcgatccg ctgcgcgcgt ttctcgccgg cggccagcgc gcggtgcggg
     3241 cctggctggc ggcgtgcaac gcctgcgcgg tggatttccc cgacgccgtc ggcgcggtca
     3301 atctcaacag cgcgctggac ctgcgcgagg cactgggctg acctggccga gggcagcagg
     3361 ccggcgcaac tggtcgaccg ccacgggcgg cgcaagcgca agctgcgcct gtcgctcacc
     3421 gaccgctgca acttccgctg ccgctactgc atgccgcagc agccgcagtg gatgaagaag
     3481 aaggacctgc tgacgcgcga cgagctgctg gcgctggcgc acctgttcgt cggcagcggc
     3541 atcgaggcca tccgcctcac cggcggcgag ccgctgctgc gcgccgacct ggtcgagtgc
     3601 gtcgccacca tcaacacgct gcgcgcgcac ggactccggc gcatttcgct caccaccaac
     3661 ggctcgcatc tcgccggccg gctggccgcg ctgcgcgccg ccgggctgga cgatctcaat
     3721 atcagcctcg atgcgctcga tcccgcgcgc ttcgccgcgc tgcgcggcgc ggacatcaag
     3781 ccggtgctcg aaggcatcgg cgccgcgctc aaccagcgcg tgccgttcaa gctcaacacc
     3841 gtgctggtgc gcggacacaa cgactgcgaa atcctgccgc tggtggactg ggccatggcc
     3901 cgcggcgcgc cgctgcgctt catcgagtac atgccgctgg acgcaccggg cacctggtcg
     3961 gcggaacagg tggtgagtgc agcgcagatc ctgcgcacgc tgcgcacgcg ccaccgcgtg
     4021 gagccgctgc tgcgcggcag cgaaccggcc tcggaatacc tgctcgacgg agaattccgg
     4081 gtcggcatca tctccaccat ctccaacccg ttctgcgcca gctgcgaccg cgtacgcctg
     4141 accgcgcgcg gcgagctcta cacctgcctg ttcgcgccgc acggcacgcc gctgggcgcg
     4201 cagctgcgcg cgggcgcgga cgcaacgatc ttgcgcaacg ccattcactc cgcggtgtgg
     4261 aacaagcaag ccggctacgc cgcgaacccg cagccggtcg aacgcccgct gctgatgcac
     4321 gctatgggag gttgaaccgg atgaacgtga cctttgaact gctcggcgtg ctgcagcgcc
     4381 tgtgcggcag cgagcgcatc cagctgcagg tgcatgcggc caccgtggcc gatgcgctgg
     4441 agcagctggc gctggcgcgg cctgacttgc gcggcgagct ggagcgctgc gcgatcgcca
     4501 gcggcgacgc catcgtgcgc cggcgcgatg cactgtcgcc gggcgcggta ctcgcgctgc
     4561 tgccgccggt ggcgggcggg tgaacgcatg agcccgcatc tcaccgaact gccgctgcag
     4621 ctgccctccc tgctggccga gggcgagtac ccgcaatgtg gcggacttgc cgtgttcggc
     4681 ggcgcggtgc gcaaccatca cgagggcaaa cccgtgctgc gcctgcgcta caccgcgtat
     4741 cgcccgctgg ccgagcggct gatgcgcgag atcgaacagc atgcacgccg gcactacggc
     4801 ctgccccatt gccgggtgca gcaccgcatc gggctgctga acatcggcga tgtcgccatc
     4861 tactgcgtga cgcgcgcgcc gcaccgcgcc gaggcgttcg atgcctgccg ctgggtggtc
     4921 gacgaagtga agcaccgcgt gccgatctgg aaggaagagt tctacgccga cggcagcagc
     4981 gccttcgtgc agggctgctg catccgcgcc gaccgcgaaa cgcatcccgc acacgatcac
     5041 gtacacgcat gaaagacgtc agcctcaaac cggacagcct gcgcaacgca cgcgccagcg
     5101 ccacactgca cgcgccggcg gcgtgcatcg agctgctgcg caagggcgag accgagaaag
     5161 gcgatgcgct gaaaaccgcg cgcgtggccg gcatcctcgc ggccaagcgc accgacgagc
     5221 tgatcccgct gtgccacccg ctgccgatcc acaaggccga cgtcacttat cagctgaagg
     5281 agacctcagt cgaggtgatt gcggaagtgg agacgatcgg gccgaccggc gtggagatgg
     5341 aagcgctgac cgcggcaagc ctcgccgcac tcacgttgta cgacatgctc aagccctacg
     5401 cgccggcgag cgaactgcgc atcagcgaca cccacctgct gcagaagaag ggcggcaagt
     5461 cgcactaccg ccgcacgctg gcgcggccgg cgagcgcggc ggtgatcgtg ctgtccgaca
     5521 ccgtcgccgc cgggcgcaag cccgacaccg ccggcgaagc cgtgcgcgag gcgctggccg
     5581 aggccggttt cgcgccgatc gcgtacgaga ttcttcccga cgaaccggcg cagctgctgc
     5641 agagcgtgca gcgcaagctg gccgacggcg tgcaactgat cgccgccgtc ggcggcaccg
     5701 gtgccgggcc gcgcgatctc acggtcgaca cgctgcgtcc gctggtgacg cgcgacatgc
     5761 ccggggtgat ggaagcggcg cgcgccttcg gccagcggcg cacgccgtac tcgatgctgt
     5821 cgcgcggcat cgccggtttc gccggcgaca gtctgctcat caccttcccc ggcagcctcc
     5881 gcggcgcgcg cgaaagcctg gccgccgtac tgccgggact ggtgcacctg ttcgatgtgc
     5941 agcgcgcgca ggcggtacac gccggcggct atagccggga ggacaactag atggacccgg
     6001 ccagcaacct gccgctgctc acgctcggca tcttcatcat cgcgctgctg tactcggccg
     6061 tgggccacgc cggcgcctcg ggctacatcg cggtgatgac gctgttcggc ctgtcggccg
     6121 cggtgatcaa gcccaccgca ctggtgctca acatcatcgt cgccagcatc accacgctgc
     6181 agttcgcccg cgccgggcat ttttcctggt gcacgttctg gccgtttgca ctgttcgcca
     6241 tgccactggc ctttgccggc gggtacctca acctgccggc gcacgtgttc aagctcatcg
     6301 tcggcgtggt gctgctggcc tcggcgctgc gcttcttcat caacccggcg gagcccgacg
     6361 aagtgacgcc gccgtcgaaa ccgctggcct tcgccgtcgg cggcggactg ggtctgctgt
     6421 cggggctcac cggcaccggc ggcggcatct tcctcacgcc gctgatgctg atcatgcgct
     6481 gggcgcacac ccggaccacc gcggcggtgt cggcgctgtt catcctgtgc aactcgatat
     6541 cggggctggc tggaaatttc tcctcgaccc ggaacctgcc ctcgctggca ctgccgctgg
     6601 ccgttgccgt ggtggtggcc ggctctctcg gctcctggct gggcagccgc aagctgccgg
     6661 tgcaggccat ccgccgcctg ctcgccgtgg tgctgctgat cgccggcggc aagctgatcc
     6721 tgacctgagc cgcgcgccat gatgccgctg gaccaggcac tggccaccta cacgcgcgaa
     6781 ctgcgcgcgc tggcgccgcg cacgctgccg ctggagcagg cgctcggcca cgtgcttgcg
     6841 gcagcggtcg agtcgcgcgt cgacctgccg ctgttcaccc agagcgcggt cgatggctac
     6901 gccgtgcgca gcgcggacct ggccggcgcc agcgccgacc gtcccgtcac gctggaactg
     6961 gccggtgaaa tcgcggccgg cagttccgcg cagcacgaac tgaccgcggg ccgcaccttc
     7021 cgcatcttca ccggcggcgc attgccggca ggcgccgatg cgtcggcgcg gcaggagatc
     7081 gtcgagcgca gcggcaaccg catcgtgctg cgccgcgcgc tcgccgccgg cgccgatacg
     7141 cgcttccgcg gtgaagagct gaaagccggt gaagtgctgg cgcagccggg gcagcgcctg
     7201 ggctccggcc tcatcgccgc gctggccatg gccggcgtca gcgcggtgcc ggtgcacccg
     7261 cggccgcgcg tggcgctgct ggtcagcggc gacgaggtgg cgagcagtgg tagtgaattg
     7321 cgcgccggcg aaatccacga cgccaacgga ccgctggtgc gcgcgtggct ggcagagcgc
     7381 ggctatccgc tggtatgggc gcgccagctg cgcgacgatc gcgccggcct tcgcgcggcg
     7441 ctggccgaag cgctcgcctg cgccgacgcg gtcatcacca ccggcggagt ttcggtgggc
     7501 gatcacgatc acctgccgcg tgcggcagcg gacgcgggcg tacgcgagct ctactggaag
     7561 gtggcgcaga agccgggcaa gccgatctgg ttcggcaccg ggattgccgg ccagccggtg
     7621 ctgtcgctgc cgggcaatcc cgccgcggtg ttgatcggac tgcacctgca tgtcagttcg
     7681 gcgctgcgtg cgctggaagg cagcacgcag gtcgaaccct catggcgcaa ggcacgcctg
     7741 gcgcgcgcgg tgaagcgcga tgagcagcgt gatcaactgc tgcgcgtctg catggcgtgc
     7801 ggcaatgacg gcagcaccac gctgacgccg ctcgggcggc aggattcgca catgctcagc
     7861 aacctgggca gcgcggacgg catggcgtgg atccgccgcg gcaccggcca gctcgaagcc
     7921 ggcgacgtgg tggagtggct ggcgctcggc taggcgccag cggcggcttc agcgctggat
     7981 gttgttgccg tagtagattt ccatgatctc gtggttgacg ttctcacgga tcttcttggc
     8041 ctcgcgcggc ggcagttccg acacgccctc gccgaacagg taggtgtcga ggtccagttc
     8101 cttcaggcgc atcttggtgt ggaagatgaa ttcctggtag acgttgacgt cgatcatctg
     8161 gtagcgatcc ttgatgtcgc ccgagatgaa gctctggata ga
//
LOCUS       DASZZC010000194         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_978694,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000194 DASZZC010000000
VERSION     DASZZC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978694"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VHE37_04235"
     CDS             complement(<1..614)
                     /locus_tag="VHE37_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT34761.1"
                     /translation="MHNSNKILYAAWLAALPAVTLAQLQPAAGPVDLVTVSADALRAN
                     AKYNAALAAFRAQKELVPQAEGKLLPQLSATGSYSYSDETLDGDYFGPLQFNNGQAQF
                     VNLPIDRNDGYKRGVYGATLTQTLFNLDQFYGWSEADVRVKQARYQLDAAQDELLVGV
                     AETYFGLLQAQANERLAQAKMADLQQQSLQVRGRAGAGLATEAD"
     gene            complement(846..1502)
                     /locus_tag="VHE37_04240"
     CDS             complement(846..1502)
                     /locus_tag="VHE37_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT34762.1"
                     /translation="MIGACALLLADDHAVVRDGLQALLETEPGFKVVAAVGNGLQAVS
                     AAQQLHPDVAVLDIAMPEMNGIEATARMATVAPRTRVVVLSMHGSSELVYRALQAGAR
                     GYVLKESAGHALVAAVRAVQAGRRFLDDRIEDVVVNGYLESERHASPLDALSPRERSI
                     LQMIVEGRSNADCARALFLSVKSVETYRSRLMQKLGIADLPALVKFAIEHGLTQLEKR
                     "
     gene            complement(1499..>2567)
                     /locus_tag="VHE37_04245"
     CDS             complement(1499..>2567)
                     /locus_tag="VHE37_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT34763.1"
                     /translation="LRSARGALLALLPPGAALGGEPQVEAGAPLQRGGWSVLASMPQR
                     LLVAPAYRATRERAAAYLAGAAVLALLIVSMYRWMRGYVDTLAAVASAVERGDWNTRV
                     PVNGPRELRGATAALNEMLDARGRMERELAASLARQQALSRRLVELHEADQQRLSREL
                     HDRIGQNLTALAVNLKIISQALAPPLAPGIAARMEDSRMLLFTTAAAVRNLITELRPS
                     ALDDYGLLSGLRWYGGLTQARSGMLTVVEGEELAPRLAPQVERTLFRIAQEALTNSVK
                     HAQARHAWVRLYDACGQVVMTVTDDGIGLDRARLLQGGAHDHWGLMMMQERAASVAAR
                     LRIDGVPGHGTRVQVEVARDA"
BASE COUNT          356 a          930 c          868 g          413 t
ORIGIN      
        1 tggtccgcct cggtggcaag gcccgcgccg gcgcgcccgc gcacctgcag cgactgttgc
       61 tgcaggtcgg ccatcttggc ctgcgccagc cgctcgttgg cctgcgcctg cagcaggccg
      121 aaataggttt ccgccacgcc gaccagcaat tcatcctgcg ccgcgtcgag ctggtagcgc
      181 gcctgcttca cgcgcacgtc ggcctcgctc cagccgtaga actggtcgag gttgaacagg
      241 gtctgcgtca gcgtggcgcc gtacacgccg cgcttgtagc cgtcgttgcg gtcgatcggc
      301 aggttgacga actgcgcctg gccgttgttg aactgcagcg ggccgaagta atcgccgtcg
      361 agcgtctcgt cgctgtagct gtagctgccg gtggccgaca gctgcggcag cagcttgccc
      421 tcggcctgcg gcaccagctc cttttgcgcg cggaacgcgg ccagcgccgc gttgtacttc
      481 gcattggcgc gcagcgcgtc ggcgctcacc gtgaccaggt cgaccgggcc ggctgccggc
      541 tgcagctgcg cgagcgtgac tgccggcagc gccgccagcc acgcggcgta cagaatcttg
      601 ttggagttgt gcatggattt tccctgtccg cactctcggg agcgcggcgc gggctagtgt
      661 acggcagcgc cgcagcggcg tcggcgcgca gcgctgtcag atagttccga tgggtgcgct
      721 gcaccggcgc ggcgtgcgga tggagcgcgt tttctccttg accgcatggt ggattccgat
      781 gttataaata atgttataaa ataatattta tattactatt tataacacgt caacgggcgg
      841 tgcggtcagc gcttttccag ctgcgtaagg ccatgctcga tggcgaactt gaccagcgcg
      901 ggcaggtcgg cgatgcccag cttctgcatc aggcggctgc gataggtctc caccgatttc
      961 accgacagga acagcgcgcg cgcgcagtcg gcgttgctgc ggccctcgac gatcatctgc
     1021 aggatgctgc gctcgcgcgg gctgagtgcg tcaagcgggc tggcgtggcg ctcgctttcc
     1081 agatagccgt tgaccaccac gtcctcgatg cggtcgtcga gaaagcgccg gccggcctgc
     1141 accgcgcgca ccgccgccac cagcgcgtgg ccggcggatt ccttcagcac gtagccgcgc
     1201 gcgccggcct gcagcgcgcg gtagacaagc tcggaactgc cgtgcatcga cagcaccacc
     1261 acgcgcgtgc gcggcgccac cgtggccatg cgcgcggtgg cttcgatccc gttcatctcc
     1321 ggcatggcga tgtcgagcac ggccacgtcc gggtgcagct gctgcgccgc gctcaccgcc
     1381 tgcaagccgt tgcccaccgc ggccaccacc ttgaacccgg gctcggtttc cagcagcgcc
     1441 tgcaggccgt cgcgcaccac ggcgtgatcg tctgccagca gcagcgcgca ggcgccgatc
     1501 atgcgtccct cgccacttcc acctgcacgc gggtgccgtg gccgggcacg ccgtcaatgc
     1561 gcaggcgcgc ggccaccgag gcggcgcgct cctgcatcat catcaggccc cagtggtcat
     1621 gcgcgccgcc ctgcagcagg cgcgcgcggt cgaggccgat gccgtcgtcg gtcaccgtca
     1681 tcaccacctg cccgcaggcg tcgtacaggc gcacccaggc atggcgcgcc tgtgcatgct
     1741 tgaccgagtt ggtcaacgcc tcctgcgcga tgcggaacag cgtgcgctcc acctgcggcg
     1801 ccagccgcgg cgccagttct tcgccttcca ccaccgtgag catgccgctg cgtgcctggg
     1861 tcaggccgcc gtaccagcgc agccccgaca gcaggccgta gtcatcgagc gccgaaggcc
     1921 gcagctcggt gatcaggttg cgcaccgcgg cggcggtggt gaacagcagc atgcgcgaat
     1981 cctccatccg cgcagcgatg cccggcgcca gcggcggcgc cagcgcctgg ctgatgatct
     2041 tcaggttcac cgccagcgca gtcaggttct ggccgatgcg gtcgtgcagc tcgcgcgaca
     2101 ggcgctgctg gtcggcctcg tgcaattcca ccagccggcg cgacagcgcc tgctgccgcg
     2161 ccagcgacgc cgccagttcc cgctccatgc gtccacgcgc atcgagcatc tcgttgagcg
     2221 cggcggtcgc gccgcgcagt tcgcgtggcc cgttcaccgg cacgcgcgtg ttccagtcgc
     2281 cgcgttccac cgcgctggcc acggcagcca gcgtgtcgac gtacccgcgc atccagcggt
     2341 acatgctgac gatgagcagc gcgagcacgg cggcgccggc caggtatgcc gcggcgcgct
     2401 cgcgggtggc gcggtaggcc ggcgccacca gcagccgctg cggcatgctg gccagcacgc
     2461 tccagccgcc gcgttgcagc ggcgcgccgg cttccacctg cggctcgccg ccgagcgccg
     2521 cgcccggcgg cagcagcgcc agcagcgcgc cgcgcgcact gcgcagg
//
LOCUS       DASZZC010000195         7118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_593909,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000195 DASZZC010000000
VERSION     DASZZC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593909"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VHE37_04250"
     CDS             <1..106
                     /locus_tag="VHE37_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34764.1"
                     /translation="NPGKIPLTLIEVQSGSYLGEDDIVRFDDVYGRVK"
     gene            complement(154..1101)
                     /locus_tag="VHE37_04255"
     CDS             complement(154..1101)
                     /locus_tag="VHE37_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 family protein"
                     /protein_id="HVT34765.1"
                     /translation="MLTPAQLRARFVAAATEKFARNVPDFAALRELVRRQGGIFLNDH
                     GAIRTADPAVCALFVRAARVLGLVRERDYEFPAKKLKSFDLQVIGEDARQFKIFVSQV
                     DLAAFPPALAQLVREDCAECAAAVDHAAFVALIGRAEDQGGLAEPHATEFVAHLVERL
                     MARNGPPLKRATLNAVAQVSGEAASALALGPDFNHVTIDVPSAGFGGIEQMASAMQAA
                     GFRLLPAIQGAGGTMLRQTATMAATMDTPVREADGSTGMAQTEKQFVEIIERAQARSA
                     DGAKLWQQDGRPLIYRNFISGNAEKIFDAASTRAADQRM"
     gene            complement(1107..2102)
                     /locus_tag="VHE37_04260"
     CDS             complement(1107..2102)
                     /locus_tag="VHE37_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVT34766.1"
                     /translation="MSTPMPGNVFLYIDFQDRRAGGNAEQALSYLERHELSGIIAKHG
                     GSVIESVSSKLVAGFVRANDALDCARILSAAAIKIRNSDPQRRNVAHRILLDYRMATA
                     DKRLQTNIAEHLSWHLPAVPPNSIVALKDFVDRVPGISPTPRLLALTQANVTANAKAR
                     SRMFLLAGERAYTEEEEETRAGSIMSVAALGLFSEIELRAGGRARKLHPTDCPISIGR
                     SKTCGFSVNVDLASRVHGTLSFENDKFTYTDASKNGSFVTLPGGEEVHLQGEKVVLAG
                     EGYISLGAPRTKQPGDVIQYICRATKLGFDEPVTDRSPSLVQDEDDATHRLPKKR"
     gene            complement(2110..3015)
                     /locus_tag="VHE37_04265"
     CDS             complement(2110..3015)
                     /locus_tag="VHE37_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT34767.1"
                     /translation="MRELVILFADLTGSTSLYEKLGDAAAKDAVQGCLQRLSALIAGQ
                     RGRVIKSLGDGVLAAFARADDAAALACRVQQESQQHQGPCAGFSFRIGFHLGAVIDDG
                     GDIFGDAVNTSSRLTTSARPGQILTTQATVLKLSPGWAVKTRAFDFEKLKGKAQPVAM
                     FELIWENTGDVTDLQSRLHKAGMGSSARSYSVLLLHAGGRSVSFTPTSPPLTIGRSAM
                     CGLVVPASHASREHARCEYSRGKFVFTDQSANGSYVLADGQHEVFLRRESIPLTGRGL
                     IGLGAPLPRGDAAGAHNHIIRFESQ"
     gene            3096..5222
                     /locus_tag="VHE37_04270"
     CDS             3096..5222
                     /locus_tag="VHE37_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HVT34768.1"
                     /translation="MYQKHCPGCFENKHGAQVCPHCGYDEAMRRLPRILAHGTMLAET
                     YRIGRVLGKPGGFGVTYLAWDSHLQQRVAIKEYLPRDIADRENGKLEVTVAEQDRAVF
                     ERGRDKFLREARIVAKLDHPSIVRVLNFFRANGTAYMVMRYYHGMTLGEYMSQRGSPL
                     PVEVAVNLLWPVIEGLQHAHQHHLLHRDIKPNNIYLTSAGVAILLDFGAAREQKEEGE
                     QTDSVMLTDRYAAIEQYHGKAQGPWTDVYGFAALLYRLTTGQLPPGALERVSFDTLAA
                     NGLIDVPEALREPLSKGLELYPEKRCDSLAEFRARLEPVMYGAPESVRHDLQQIYDTV
                     GEVRRVQSAAGQAPAAQEPVLPEAPVTEVSASTSASESASEMPTVQAEAVTAAPRSVA
                     KPKSAPAAPRSWRWPLAGTAAAIVLLAAGAVLMRRAPHAIPPLTTGDVLSTAPADAPQ
                     AAEIARAPGDDSILAVRAPRMVRLRGGDFRLGSDAPLARTNEQPPQPVHLKPFAIALA
                     EVTRGEFRAFVEHAHYDNPFWRDYPCDDPAGAGASWQHPGGGGEDQPVVCVSAADARA
                     YAAWLAQALRKPYRLPTEAEFEYAQRGGSGEPWWWGNGPVAGHAVCADCSAVAPQGPS
                     VAAGGPVNPYGLYGAAGNVREWTCSEYQPLAAGAWEHCAARPDILTAIRGGSWHDGMA
                     ALRSSYREVLFGHQRDVYTGFRLAQD"
     gene            5268..6701
                     /locus_tag="VHE37_04275"
     CDS             5268..6701
                     /locus_tag="VHE37_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVT34769.1"
                     /translation="MQKKTEQDKNSSFYVDLEKLLRQQLQVEPRSKDLRLKLLELYFD
                     TSRSEDFLREARNLAQDIRNKSSSAEWQRAANMGRMLLPDHDLFRLEIADKVEFIEQS
                     APAVRQRVKKRFGEGHAAYFERLGKDYDAVRTDPKFLADLDLQLVHVANRPSSLMHAQ
                     RLSEYLGGAQIYFKREDLSPAGTYLTISVCGQALLAKKLGRTTLVSASSNGLRGVITA
                     SVAARLGLKCVIYMDGKDMHLQSSNVFRMWLMGATVTAADPHTYHKGDVRETALEHCA
                     RDGSCFMVLGLDAAPQPYPMMGLEFAAVIGRETRRQLAGARRVPSLLVARAGENADAI
                     GFFQPFLREPQPRLACVATRSEFTREASATHDPFKAPLSAAEKLRAGVIMEGLEYPSV
                     TREHAWLKASGRVDYVDISEDAVKKAIGDCGRLEGLIPPIQTAHAIAWAIAEARRMEP
                     TETVAVILRESMDRDIWEIGRAMGVPL"
     gene            complement(6821..>7118)
                     /locus_tag="VHE37_04280"
     CDS             complement(6821..>7118)
                     /locus_tag="VHE37_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT34770.1"
                     /translation="RAIGFGAAAVIVSVLLEALLLALIGGAIGAVIAYFVFNGYKVST
                     LNMQTFSQVAFAFRVTPELLVQGVIWACVIGLIGGFFPALKAARLPIVDALRAN"
BASE COUNT         1178 a         2460 c         2421 g         1059 t
ORIGIN      
        1 aaacccgggg aagatcccgc tgacgctgat cgaggtgcag tccggctctt acctgggcga
       61 ggacgacatc gtgcgcttcg acgacgtgta cgggcgtgtg aaataaacgg tccgattatc
      121 caaaaattac cggatcagca atcgctcgca ccgctacatg cgctgatcgg ccgcccgcgt
      181 cgaagcggca tcgaagatct tctcggcatt gcccgagatg aaattgcggt agatcagcgg
      241 gcgtccatcc tgctgccaca gtttcgcgcc gtcggcgctg cgcgcctgcg ctcgctcgat
      301 gatctctacg aactgcttct cggtctgcgc catgccggtt gaaccgtcgg cctcgcgcac
      361 cggcgtgtcc atggtcgccg ccatggtcgc ggtctggcgc agcatggtgc cccccgcgcc
      421 ctggatcgcc ggcagcaggc ggaaaccggc cgcctgcatg gccgaggcca tctgttcgat
      481 gccgccgaag ccggctgacg gcacatcgat ggtcacgtgg ttgaagtccg gccccagcgc
      541 cagcgcgctt gccgcctcgc cggaaacctg cgccaccgcg ttgagcgtgg cgcgcttcag
      601 cggcgggccg ttgcgtgcca tcagacgttc gaccaggtgt gctacgaatt ccgtagcatg
      661 cggctcggcc aggccgccct ggtcctcggc acgcccgatc aatgcgacga aagcagcgtg
      721 atccactgct gccgcgcatt cggcgcagtc ctcccgcacc agctgggcca gcgccggcgg
      781 aaacgcggcc agatccacct gcgagacgaa gatcttgaac tggcgtgcat cctcgccgat
      841 cacctggagg tcgaatgact tcagcttctt ggccggaaac tcgtagtcgc gctcgcgcac
      901 cagccccagc acgcgcgcgg cgcgcacgaa cagcgcgcac acggcgggat ccgccgtgcg
      961 gatggcaccg tggtcgttga ggaagatccc gccctggcgc cgcaccagct cgcgcagcgc
     1021 ggcgaagtcc ggcacgttgc gcgcaaattt ctcggtggcg gcggcgacaa aacgggcgcg
     1081 cagctgggcg ggagtcagca tggccttcac cgcttcttcg gcagacgatg ggtggcgtcg
     1141 tcctcgtcct gcaccagcga cgggctgcgg tcggtgacgg gttcatcgaa ccccagcttg
     1201 gtggcgcggc agatgtactg gatcacgtcg cccggctgct tggtgcgcgg cgcgcccagg
     1261 ctgatgtagc cctcgccggc cagcaccacc ttctcgcctt gcaggtgcac ttcctcgccg
     1321 cccggcaggg tgacgaagct gccgttcttg ctggcatcgg tgtaggtgaa cttgtcgttc
     1381 tcgaagctca gggtgccgtg cacgcgcgaa gccaggtcga cgttgaccga gaagccgcag
     1441 gtcttgctgc ggccgatgct gataggacaa tcggtcgggt gcagcttgcg cgcacgcccg
     1501 ccggcgcgga gttcgatttc cgagaacagg cccagcgccg ccaccgacat gatcgagccg
     1561 gcgcgcgtct cctcctcttc ctccgtgtac gcgcgctcgc cggcgagcag gaacatgcgc
     1621 gaacgggcct tggcgttggc ggtgacgttg gcctgggtca gcgccagcag ccgcggcgtc
     1681 ggactgatgc ccggcacgcg gtcgacgaaa tccttcagcg cgacgatgct gttgggcggc
     1741 accgccggca ggtgccacga caggtgctcg gcgatgttgg tctgcaggcg cttgtcggcc
     1801 gtggccatgc ggtaatcgag caggatgcga tgggccacgt tgcgccgctg cggatcgctg
     1861 ttgcggatct tgatcgccgc ggcactgagg atgcgtgcgc agtccagcgc gtcgttggcg
     1921 cggacgaagc ccgccaccag cttgctcgac accgattcga tcacgctgcc gccgtgcttg
     1981 gcgatgatgc cgctcagctc atggcgctcc aagtagctga gcgcctgctc ggcgttgccg
     2041 ccggcccggc ggtcctggaa atcgatgtag agaaacacgt tgcctggcat cggtgtgctc
     2101 atggccgcct cactggctct cgaaacggat gatgtggttg tgcgcgcccg ccgcgtcccc
     2161 gcgcggcagc ggcgcaccca ggccgatcag cccgcggccg gtcagcggta tcgattcgcg
     2221 ccgcaggaac acctcgtgct ggccgtcggc cagcacgtag ctgccgttgg cgctctggtc
     2281 ggtgaacacg aacttgccgc ggctgtactc gcagcgcgcg tgctcgcgcg aggcgtgcga
     2341 ggccggcacc accagcccgc acatggcgga gcggccgatg gtcagcggcg ggctggtcgg
     2401 ggtgaagctg acgctgcggc cgccggcgtg cagcagcagc acgctgtagc tgcgcgcgct
     2461 gctgcccatg ccggccttgt gcaggcggct ctgcaggtcg gtgacgtcgc cggtgttttc
     2521 ccagatcagc tcgaacatcg ccaccggctg cgccttgccc ttgagctttt cgaaatcgaa
     2581 tgcgcgcgtc ttcaccgccc agcccggcga cagcttgagc acggtggcct gggtggtgag
     2641 gatctgcccc ggacgcgcgc tggtggtcag gcgcgagctg gtgttgaccg cgtcgccgaa
     2701 gatgtcgccg ccatcgtcga tcaccgcacc caggtggaag ccgatgcgga aggaaaagcc
     2761 ggcgcagggc ccctggtgct gctggctttc ctgctgcacg cggcaggcca gtgccgcagc
     2821 atcgtcggcg cgggcaaagg cggcaagcac gccgtcgccc aggctcttga tcacgcggcc
     2881 gcgctggccg gcgatcagcg ccgacagccg ctgcagacag ccctgcacgg cgtctttggc
     2941 agccgcgtcc cccagcttct cgtagagcga ggtgctgccg gtgaggtccg cgaacaggat
     3001 gaccaactca cgcatgtgcg ttcgggagat aagaactcgg tcagtataat ggggaaaaaa
     3061 tcaccgccgc acgaccggcc agaggcttac ggcgcatgta tcagaagcat tgcccaggct
     3121 gcttcgagaa caagcatggc gcacaggtgt gcccgcattg cggctacgac gaggccatgc
     3181 gccggctgcc gcgcatcctg gcccacggca ccatgctggc cgagacctac cgcatcggcc
     3241 gcgtgctcgg caagcccggc ggcttcggcg tcacctacct cgcctgggac agccacctgc
     3301 agcagcgcgt ggcgatcaag gaatacctgc cgcgcgacat cgccgaccgc gagaacggca
     3361 agctcgaggt gacggtggcc gagcaggacc gcgcggtgtt cgagcgcggc cgcgacaagt
     3421 tcctgcgcga ggcgcgcatc gtggccaagc tcgatcaccc gtcgatcgtg cgcgtactca
     3481 acttcttccg cgccaacggc accgcttaca tggtgatgcg ctactaccat ggcatgacgc
     3541 tgggcgaata catgagccag cgcggctcgc cgctgccggt ggaagtggcg gtgaatctgc
     3601 tgtggccggt gatcgagggc ctgcagcacg cgcaccagca ccacctgctg caccgggaca
     3661 tcaagccgaa caacatctac ctcacctccg ccggcgtggc catcctgctc gacttcggcg
     3721 cggcgcgcga acagaaggag gaaggcgagc agaccgactc ggtgatgctc accgaccgct
     3781 acgccgccat cgagcagtac cacggcaagg cacagggccc gtggaccgac gtctatggct
     3841 tcgccgccct gctctaccgc ctgaccaccg gccagctgcc gccgggcgcg ctcgagcgcg
     3901 tgtcgttcga cacgctcgcc gccaacggcc tgatcgacgt gcccgaggcg ctgcgcgagc
     3961 cgctgtcaaa aggcctcgag ctgtacccgg aaaaacgctg cgacagcctg gcggaattcc
     4021 gtgcgcgcct ggaaccggtg atgtacggcg cgcccgagtc ggtgcgccac gacctgcagc
     4081 agatctacga caccgtgggc gaggtgcgcc gcgtgcagag cgccgccggc caggcgccgg
     4141 ccgcgcagga accggtgctg ccggaggcac cggttacgga agtctccgct tccaccagcg
     4201 ccagcgaatc ggccagcgag atgcccacgg tgcaggccga ggccgtcact gccgcgccgc
     4261 gcagcgtggc gaaaccaaaa tccgcgccgg cggcgccacg ctcgtggcgc tggccgctgg
     4321 cgggcacggc agccgcgatc gtgctgctgg cggcgggcgc cgtgctgatg cgccgtgccc
     4381 cgcatgcaat cccgccgctg accaccggcg acgtgctcag cacggcaccg gccgacgcac
     4441 cgcaggcggc cgagatcgcg cgcgcgccgg gcgacgacag catcctggcg gtgcgcgcgc
     4501 cgcgcatggt gcgcctgcgc ggcggcgact tccgcctggg cagcgacgcg ccgctggccc
     4561 gcaccaacga gcagccgccg cagcccgtgc acctcaagcc gttcgccatc gcgctggccg
     4621 aagtcacgcg cggcgaattc cgcgccttcg tcgagcacgc gcactacgac aatcccttct
     4681 ggcgcgacta tccctgcgac gatcctgccg gcgccggcgc cagctggcag catccaggcg
     4741 gcggcggcga agaccagccg gtggtctgcg tcagcgcggc tgatgcgcgc gcctatgcgg
     4801 cctggctggc gcaggcgctg cgcaagccct accgcctgcc caccgaggcc gagttcgagt
     4861 acgcgcagcg cggcggcagc ggcgagccct ggtggtgggg taacgggccg gtagccggcc
     4921 acgcggtgtg cgccgactgc tcggcagtcg cgccgcaggg gccgtccgtc gcggccggcg
     4981 gcccggtcaa tccctacggc ctgtacggtg cggccggcaa cgtgcgcgaa tggacctgct
     5041 cggagtatca gccgctggcc gccggcgcct gggaacattg cgctgcgcgg cccgatatat
     5101 tgaccgccat ccgcggcggc agctggcatg acggcatggc ggcgctgcgc agcagctacc
     5161 gcgaggtgct gttcgggcat cagcgcgatg tgtataccgg gttccggctg gcgcaggact
     5221 gacagcgggc gccgccgctg gtctaatcgg cagtgcaagg agggcagatg cagaagaaga
     5281 ccgagcagga caagaacagc agcttctacg tggacctgga aaagctgctg cgccaacaac
     5341 tgcaagtcga gccgcggtcc aaggacctgc ggctcaagct gctggagctg tacttcgaca
     5401 ccagccgcag cgaagacttc ctgcgcgaag cgcgcaacct ggcgcaggac atccgcaaca
     5461 agtcgtcctc ggccgagtgg cagcgcgctg ccaacatggg ccgcatgttg ctgccggacc
     5521 acgacctgtt ccggctggag atcgccgaca aggtggagtt catcgagcaa agcgcgccgg
     5581 cggtgcgcca gcgcgtgaag aaacgcttcg gcgaaggcca cgcggcctac ttcgagcggc
     5641 tgggcaagga ctacgacgcg gtgcgcaccg accccaagtt cctcgccgat ctcgacctgc
     5701 agctggtgca cgtggccaac cggccatcgt cgctgatgca cgctcagcgc ctgtcggaat
     5761 atctcggcgg cgcgcagatc tacttcaagc gcgaggacct gtcgccggcc ggcacctatc
     5821 tcaccatcag cgtctgcggc caggcgctgc tggccaagaa actcgggcgc accacgctgg
     5881 tctcggccag cagcaacggc ctgcgcggcg tgatcaccgc ctcggtggcc gcgcgcctgg
     5941 gcctgaagtg cgtgatctac atggacggca aggacatgca cctgcagtcg tccaacgtgt
     6001 tccgcatgtg gctgatgggc gccaccgtca ccgcggccga tccgcacacc taccacaagg
     6061 gcgatgtgcg cgagaccgcg ctggaacact gcgcgcgcga cggcagctgc ttcatggtgc
     6121 tgggcctcga cgccgcgccg cagccctatc cgatgatggg cctggaattc gccgcggtga
     6181 tcggccgcga gacgcgccgc cagctggccg gcgcgcgccg cgtgccctcg ctgctggtgg
     6241 cgcgcgccgg cgagaacgcc gacgccatcg gcttcttcca gccgttcctg cgcgagccgc
     6301 agccgcggct ggcgtgcgtg gccacgcgca gcgaattcac ccgcgaggcg agcgccacgc
     6361 acgacccgtt caaggcgccg ctgtccgccg ccgagaaact gcgcgccggc gtgatcatgg
     6421 aaggcctcga gtaccccagc gtcacccgcg agcacgcctg gctcaaggcc agcgggcggg
     6481 tcgactacgt cgatatttcc gaagacgcgg tgaagaaggc catcggcgac tgcggccgcc
     6541 tggaagggct gatcccgccg atccagacgg cgcacgccat cgcctgggcc attgccgagg
     6601 cgcggcgcat ggagccgacc gaaaccgtcg ccgtgatcct gcgcgaaagc atggaccggg
     6661 acatctggga gatcggccgc gccatgggcg tgcccctgta ggccgcaaaa acactccacc
     6721 gcacggccgt tgacgctgtt ataaaaaata tactgaaagt aaattcagac aataattaat
     6781 aacacaccag gaggcggtgc ggtggcggca aaaacagcgt tcagttggcg cgcagcgcgt
     6841 ccacgatggg caggcgcgcc gccttgagcg ccgggaagaa tccgccgatc aggccgatga
     6901 cgcaggccca gatcacgccc tgcaccagca gctcgggcgt gacgcggaag gcgaacgcca
     6961 cctgcgagaa ggtctgcatg ttcagcgtcg acaccttgta gccgttgaac acgaagtagg
     7021 cgatcaccgc gccgatggcg ccgccgatca gcgccagcag cagcgcttcc agcagcaccg
     7081 acacgatcac cgcggcggcg ccgaagccga tcgcgcgc
//
LOCUS       DASZZC010000196         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1172378,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000196 DASZZC010000000
VERSION     DASZZC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172378"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VHE37_04285"
     CDS             complement(<1..1018)
                     /locus_tag="VHE37_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVT34771.1"
                     /translation="MAITRRNLIKHSGLASLAALGGGWSALAHAGERLIPWRNWSGGQ
                     ACVPEARLAPRDEVELAEMVKGATGNVRAVGSGHSFSPLVPTDGTIVSLSNLPAGVIG
                     HNASALTAEIWAGTRIADLGAPLKAIGQALPNMADIDYQTLAGAISTSTHGTGPKYGS
                     YSTRVIGLRMVTAKGEILDVDTQNHPEIFGAARVSLGALGVVTRVRLQNRKAFRLHRR
                     EWVADVEDLLAQMPQLIAENDHFEINALLHSDVAVATALNEATDNRTIEKEMGGDVAK
                     VKLLQIVDRWAGDTDGLQAALLNFTAKHLISFPEVIDDSWKVFANVRDVRFNEMEYSV
                     PAEAG"
     gene            complement(1027..1365)
                     /locus_tag="VHE37_04290"
     CDS             complement(1027..1365)
                     /locus_tag="VHE37_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT34772.1"
                     /translation="MKRCTLLLALLLCACGGDKKPAASAPLPPPADPALAKVFDSTCR
                     TCHANPASGAPQAGDTAAWSKRVAQGKDTLLTHTMSGFQSMPPMGLCMQCDEAQFTAL
                     IEYMSGAKLQ"
     gene            1499..>1729
                     /locus_tag="VHE37_04295"
     CDS             1499..>1729
                     /locus_tag="VHE37_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34773.1"
                     /translation="MQIHINKWLAACCAALLVASCGHNSIPGSIDNGGNNGGGALSGN
                     LGPGDGDSIYLNVLPPGANGNSSGGIGVPVGGT"
BASE COUNT          256 a          581 c          605 g          287 t
ORIGIN      
        1 ggccggcttc cgccggcacc gagtactcca tctcgttgaa gcgcacgtcg cgcacgttgg
       61 cgaacacctt ccacgagtcg tcgatcacct cgggaaacga gatcaggtgc ttggcggtga
      121 aattcagcag cgccgcctgc aggccgtcgg tgtcgccggc ccagcggtcg acgatctgca
      181 gcagcttgac cttggccacg tcgccgccca tctccttctc gatggtgcgg ttgtcggtgg
      241 cctcgttgag cgcggtggcc accgccacgt cggaatgcag cagcgcgttg atctcgaaat
      301 gatcgttctc ggcgatcagc tgcggcatct gcgccagcag gtcttcgaca tcggccaccc
      361 attccctgcg gtgcaggcgg aaggccttgc ggttctgcag gcgcacgcgc gtgaccacac
      421 ccagcgcgcc gagagagacg cgcgcggcgc cgaagatctc cgggtgattc tgcgtgtcga
      481 cgtcgaggat ctcgcccttg gcagtgacca tgcgcaggcc gatcacgcgg gtggagtacg
      541 agccgtactt cggcccggtg ccgtgggtcg aggtgctgat ggcgccggcc agagtctgat
      601 agtcgatgtc cgccatgttg ggcagcgcct ggccgatggc cttcagcggc gcgccgagat
      661 cggcgatgcg cgtgccggcc cagatttccg cggtgagcgc ggaggcgttg tggccgatga
      721 cgccggccgg caggttggac agcgagacga tggtgccgtc ggtcggcacc agcggcgaga
      781 aggaatggcc cgagcccacc gcacgcacgt tgccggtggc gcccttcacc atctcggcca
      841 gttccacttc atcgcgcggc gccagccgcg cctccggcac gcaggcctgg cccccggacc
      901 agttgcgcca cgggatcagc cgctcgccgg catgcgccag cgcgctccag ccgccgccga
      961 gcgcagccag gctggcgaga ccgctgtgct tgatcaggtt cctgcgggtg atggccatgc
     1021 gcgctcctac tgcagtttcg cgccggacat gtattcgatc agcgcggtga actgcgcctc
     1081 gtcgcactgc atgcacaggc ccatcggcgg catgctctgg aagccgctca tggtgtgcgt
     1141 gagcagcgtg tccttgccct gtgccacgcg cttgctccac gcggcggtgt cgccggcctg
     1201 cggtgcgccc gacgccgggt tggcatggca ggtgcggcag gtgctgtcga acaccttggc
     1261 cagcgccggg tcggccggcg gcggcagcgg cgcggaggcg gccggcttct tgtcgccgcc
     1321 gcaggcgcac agcagcaggg cgagcagcag ggtgcagcgc ttcattggga ctcccggtgg
     1381 atcttttgcg tgggcgaatt gtgaagcaag cggcgcgccc gtgcgcgagt aaagcatgct
     1441 ccaccgcggc cgccgcaatg ctactgttcg gcggccaaaa aatgcgagga gtgccgccgt
     1501 gcaaatccac atcaataaat ggctcgcggc gtgttgcgcc gccttgctgg tcgcctcctg
     1561 cggacacaac tcgattcccg gctcgatcga caacggcggc aacaacggcg gcggcgcgct
     1621 gagcggcaac ctgggaccgg gcgatggcga cagcatctac ctcaacgtgc tgccgcccgg
     1681 cgccaatggc aattcctccg gcggcatcgg tgtgccggtg ggcggcacg
//
LOCUS       DASZZC010000197         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_851374,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000197 DASZZC010000000
VERSION     DASZZC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851374"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VHE37_04300"
     CDS             <1..376
                     /locus_tag="VHE37_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HVT34774.1"
                     /translation="FVDGQDTPIVTDSIHAAGTVDIYVRPGDLRLAEDGGPGFEAIVR
                     STQRTGPILRCEAVTTGSNVPIEIEVPHLHHDAQQLRRDARVRLRLNQFSIFTSRGRT
                     NAAKPVEAPVLIGSERERGRLG"
     gene            600..1064
                     /locus_tag="VHE37_04305"
     CDS             600..1064
                     /locus_tag="VHE37_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34775.1"
                     /translation="MKIRGVTRIEGPQGRGSGWMVRLTRNGKTTHKWFADGLHGGKRK
                     ALAAAREHQARLEAAAPPVSSPAERKTVRNTSGKVGVRLEQKTTVRGAREYEYEGYSA
                     FWRTPEGSTQTITFSCSKYGRRKARQLASIARDHQRADRSWVERRHASAAGN"
     gene            1074..1442
                     /locus_tag="VHE37_04310"
     CDS             1074..1442
                     /locus_tag="VHE37_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34776.1"
                     /translation="MGRALLRPLLCLTLIVCILASGQSPASLTLVTGASGFFVVIYTQ
                     LARGSRAVALGWVLLVTFVLPVAMLFALSHQAVGSWGDSIVYLGRSIRDHGVVTSLVT
                     LTAPSAAAGIAAMLVSRHAL"
     gene            complement(1446..>2063)
                     /locus_tag="VHE37_04315"
     CDS             complement(1446..>2063)
                     /locus_tag="VHE37_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVT34777.1"
                     /translation="YRIVPGQVLRLSAPPPSASAHTESGSAAPTPLPPPVAATHAPVP
                     VPVESATVSPTATAVASAAPPVEASTVDTPVELPAGFQWQWPTAGSVLHAYAPASGAK
                     GLDFTGNIGQPVLAAAPGRVVYSGNALKGYGELIIIKHDEQHLSAYGYNRKRYVNEGD
                     FVTAGQPIGELGLGPENKPLLHFEIRERGKPVNPSQYLPPRASSG"
BASE COUNT          322 a          703 c          714 g          324 t
ORIGIN      
        1 gttcgtcgac ggccaggaca cgccgatcgt caccgacagc atccacgctg ccggaacggt
       61 cgacatctac gtgcgccccg gcgacctgcg cctggccgag gacggcgggc ccggtttcga
      121 agccatcgtg cgcagcacgc aacgcaccgg gccgattctg cgctgcgaag cggtgaccac
      181 cggctcgaac gtgcccatcg agatcgaagt gccgcacctg caccacgacg cgcagcagct
      241 gcggcgcgac gcgcgcgtac gcctgcgcct gaaccagttc agcatcttca cctcgcgcgg
      301 gcgcaccaac gctgccaagc cggtggaagc gccggtgctg atcggcagcg agcgcgaacg
      361 cgggcggctg ggctgatttt tgccttcacc gcaccccggc gatgactgtt ataaaagata
      421 tattttattt atatttataa tattttttat aacacgacaa atgcacttgc ggtggagctt
      481 tcccgggccg cgttccgact aacaacgcgt tgccgaggac agcgggacac cgctgcaggt
      541 tccgcggacg gcgcattccg ctctagaatc cgccttccct cagcatcgaa ggaccgagca
      601 tgaaaatccg aggagtgacg cgcatcgaag gcccgcaagg acgcgggtcg ggctggatgg
      661 tgcgcctgac gcgcaacggc aagaccacgc acaagtggtt cgccgacgga ctgcacggcg
      721 gcaagcgcaa ggcgctggca gcagcacgcg aacaccaggc gcgtctcgag gcggccgcgc
      781 cgccggtgtc atcgccggcc gagcgcaaga ccgtgcgcaa caccagtggc aaggtcggcg
      841 tgcgcctgga gcagaaaacc acggtgcgcg gcgcacgcga atacgagtac gaaggctact
      901 cggcgttctg gcgcacgccg gaaggctcca cgcagaccat caccttctcc tgcagcaagt
      961 acggccggcg caaggcgcgg cagctggcca gcatcgcgcg cgatcaccag cgcgcggacc
     1021 gcagctgggt cgagcgccgc cacgccagcg ccgcgggaaa ctgagctgct ggcgtgggcc
     1081 gcgcgctgct ccgaccgctg ttgtgcctga cgctgatcgt ctgcatcctg gccagcggcc
     1141 agtcgccggc gtcgctgacg ctggtgacgg gcgcatcggg cttcttcgtg gtgatctaca
     1201 cccagctcgc gcgcggcagc cgcgcggtag cgctgggctg ggtgctgctg gtgaccttcg
     1261 tgctgccggt ggcgatgctg ttcgcgctca gccaccaggc ggtcggctcc tggggcgatt
     1321 ccatcgtcta cctgggtcgc tccatccgcg accacggcgt ggtgacgtcg ctggtcacgc
     1381 tcaccgcgcc cagcgccgcc gccggcatcg cggccatgct ggtcagccgc cacgcgctgt
     1441 aacgctcagc ctgagctggc gcgcggcggc aggtattgcg agggattgac cggcttgccg
     1501 cgctcgcgga tctcgaagtg cagcagcggc ttgttctccg ggcccaggcc cagttcgccg
     1561 atcggctggc cggcggtaac gaaatcgccc tcgttgacgt agcgcttgcg gttgtagccg
     1621 taggcggaca ggtgctgctc gtcgtgcttg atgatgatga gctcgccgta gcccttgagc
     1681 gcgttgccgc tgtagaccac gcggcccggc gccgcggcga gcaccggctg gccgatgttg
     1741 ccggtgaagt ccaggccctt ggcgccgctc gccggcgcat acgcgtgcag cacggagccg
     1801 gcagtgggcc attgccactg gaagccggcg ggcaattcca ccggcgtgtc gaccgtgctg
     1861 gcctcgaccg gtggcgcggc cgatgcgacc gccgttgccg tcggcgatac cgtcgctgat
     1921 tccaccggta ccggcacggg cgcgtgcgtg gctgccacgg gcggcggcag cggcgtgggc
     1981 gccgcactgc cgctctcggt gtgtgcgctt gccgatggcg gcggcgcgct caggcgcagc
     2041 acctgcccgg gcacgatgcg gta
//
LOCUS       DASZZC010000198         6545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_396585,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000198 DASZZC010000000
VERSION     DASZZC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396585"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VHE37_04320"
     CDS             complement(<1..1393)
                     /locus_tag="VHE37_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVT34778.1"
                     /translation="MRARGAWLACAALLASCGGGGGLQTSSCDGHCADSGARLEIADV
                     QRVLAQSVNEAKARGAKATIAVVDRVGNVLSVYRMSGAAASVTIDSGRGVSGGLEQVA
                     IIPDSMAAISKALTGAYLSSEGNAFSTRTASQIVQEHFLPQENNQPSGPLFGVQFSSL
                     PCSDLSTRYNGTAPSVGPQRAPLGLSADPGGFPLYKNGALIGGVGVISDARYGLDLDI
                     SDTDRDADELIALAGTAGYAPPDDRRADRITADGKTFRYSDAGYGDLAADPANAPALL
                     PTDGATISVAGYFDATQPAAPGLAFGTAASGIRPATGVLGSLDGFVLVDAGNAERYPA
                     RDATDAAVTGASKLLSAEVQQLLASALDIAHRARAQIRRPLGSSATVSISVVDTYGQV
                     LGIVRSRDAQVFGIDVSLQKARTAAFFSNTAAAADLAATPDTTYFATSQAASISAYLP
                     ALRTFLGQPNALAD"
     gene            complement(1393..3036)
                     /locus_tag="VHE37_04325"
     CDS             complement(1393..3036)
                     /locus_tag="VHE37_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVT34779.1"
                     /translation="MRVVVRTLSMNGNQVVWTERTLDTERLRIGRGTEQDVELGDIRV
                     ALAHAEIVAGRPYKLTARPGATVWVNDAPLHDADLNIGDVIDIGRYRLTVLSPELGTD
                     LLISVQEWISAREEKQQRLRALQTRLEHTGFSRRRWAWWLALAVLLPGLLLPVWLRYH
                     VNAADPGDQVWLPGPLSRAHAQFGDNCSACHGAPFERVRNSACAACHAGVHQHTDNPA
                     LLGQVQFAKARCAACHYEHHGDHGLLLPHPAICTDCHGQPEAGLTDTRVPMVADFMRS
                     HPPLRASVARYSGGTFAIMRVEPGSADWQQQLNLRFPHDRHLLASGIDSPQGRKQLHC
                     ADCHQPDSSGKNFAPVSMQAHCSGCHKLDFDPAAPTRELPHAKPQEVVQVIRDYYAQR
                     ALHGGDPQRHDDGELRRVPGPGETPSPADLAWAERRARQAIDDVFDRRVCVYCHLVER
                     TGDAQLPWRIAPVAPWLAGYPHAKFDHAAHRMQKCESCHAARASSNSSDVLLPDIASC
                     RGCHGDPGQPERVPSTCTSCHGFHIARTQLMTPARKGVR"
     gene            complement(3040..4011)
                     /locus_tag="VHE37_04330"
     CDS             complement(3040..4011)
                     /locus_tag="VHE37_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34780.1"
                     /translation="MAALHQGFLSYRQFAFLKLAALLCVAGLFAYAAHDPPEGPSGGS
                     WLGYTLGTLGALIIVWLAWLGVRKRRYARGIGSVKAWVSAHVYLGLSLLIIATLHTGF
                     RFGWNVHTLSYLLMLAVIASGIYGVLAYARYPELITRNSGDAMLESWLAEIQDLNDQA
                     LKIADTLGPDVHARVLRSNERVRVGGSAWQQLIGRTRPRAAAAAEMLNQNLSERMQAA
                     RAPVSKPGLGDFASSSQSTVMFMADQLVAGLDEAPAKAPAKGNSEADRIRKLLDLLAR
                     RQELVARANRDITLTARMQVWLYLHVPLTFALLAALAAHIVSVFLYW"
     gene            complement(4144..>6545)
                     /locus_tag="VHE37_04335"
     CDS             complement(4144..>6545)
                     /locus_tag="VHE37_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVT34781.1"
                     /translation="DHYKIAVVGSGPAGLSAAAHAAELGVSHVLLEAAPHVSNTIFRY
                     QKGKHVMAEPGVLPLRSPLPFAAGRREEVLGAWNDGVAQHQVNVRHRAEVIAITGGQG
                     AFVLTLGDKSQVSAQFVVLAVGLQGNIRKLGVPGEDLPFVQYQLDDPDEYAGERIIVV
                     GVGDAGLENALALAERNQVTILNQRDDFDRAKEGNRTAILKAIADGTVQCLYNTRVAE
                     VQARAGKPRGAMLVNTAAGKSELPCDRVIARLGAMPPRKFVESAGITFPSADPNAVPA
                     VSSTYESNVPGLYIIGALAGFPLIKQAMNQGYEVVEFIEGRAVEPADAGLLREKFAAV
                     PQWGSVDEAIARIRQRVPVLAPLTPLQLREFLLDSTLHAPAPGTVIFERNDYSNSFYS
                     VIDGSVEVDTSGESGRRISIGAGEFFGEIGLISGRRRTATVRAGADCVLLETPRRSMS
                     KLIASVAAVKRVVDEAFLRRAIQARIAPGVSTQDLAQVLASARVCQFNAGDVLFREGE
                     AGDALHLIRKGSVTVSRMLGGREVVLAYVPAGQYVGEMALVSSQPRSATARAAVATET
                     IRVEGEAFKALMQRHPQIEELIAQLYRERLAQNVAMQETPQFGGVINFLMAQGFGEAT
                     DVLLIDESLCIRCDNCEKACADTHHGLSRLNREAGPTFDQLHVPTSCRHCEHPHCMKD
                     CPPDAIKRDENGEVYITDACIGCGNCQRNCPYGVIQMGVEADARPSLWRYFMFGGAEP
                     GRATAHKKGKSDKQKKAAKCDMCRDLPQGPSCVRACPTGAAIRVGPEEFINVLKAQTV
                     E"
BASE COUNT          941 a         2243 c         2324 g         1037 t
ORIGIN      
        1 cgtcggccag cgcattgggc tggccgagaa aagtccgcag cgcgggcagg tacgcggaaa
       61 tgctcgccgc ctggctggtg gcgaagtagg tggtgtccgg cgtcgcggca agatcggccg
      121 ctgccgccgt gttggaaaag aacgcggcgg tgcgcgcttt ctgcagcgag acatcgatgc
      181 cgaacacctg cgcgtcgcgc gagcgcacga tgcccagcac ctggccgtag gtatcgacca
      241 ccgagatgct gaccgtggcc gaggagccca gcggccggcg gatctgcgca cgggcgcggt
      301 gcgcgatgtc gagcgcgctg gccagcagct gctgcacttc cgccgagagc agcttgcttg
      361 cgccggtgac cgcggcatcg gtggcatcgc gcgccgggta gcgctcggca ttgccggcgt
      421 ccaccagcac gaagccgtcg agcgagccga ggacgcccgt ggccggtcgt atccccgagg
      481 ccgcggtgcc gaaggccagc cccggcgcgg ccggctgcgt ggcgtcgaag tagcctgcta
      541 cggaaatcgt agcgccgtcg gtcggcagca gcgccggcgc atttgccggg tcggccgcca
      601 ggtcgccgta gccggcgtcg ctgtagcgga aggtcttgcc gtcggcggta atgcggtcgg
      661 cgcggcggtc gtcgggcggc gcgtagccgg cggtgccggc cagcgcgatc agctcgtcgg
      721 cgtcgcggtc ggtgtcgctg atgtcgaggt cgagcccgta acgcgcgtcg ctgatcacgc
      781 ccacgccgcc gatcagcgcg ccgttcttgt acagcgggaa gccgccggga tcggcggaca
      841 gccccagcgg cgcgcgctgc gggccaaccg acggcgccgt gccgttgtag cgcgtcgaca
      901 ggtcggagca gggcagcgag gaaaactgca cgccgaacag cgggccggac ggctggttgt
      961 tttcctgcgg caggaaatgc tcctgcacga tctggctcgc ggtgcgcgtg gaaaacgcat
     1021 tgccctccga cgagagatag gcgccggtca gcgccttgga aatcgcggcc atgctgtcgg
     1081 gaatgatggc gacctgttcc agtccgccgc tcacgccgcg gccggagtcg atggttacgc
     1141 tcgccgccgc gccgctcatg cggtacacgc tgagcacgtt gccgacgcgg tcgaccacgg
     1201 caatggtcgc cttcgctccg cgcgccttcg cctcgttcac cgactgcgcg agcacgcgct
     1261 gcacgtcggc gatctccagc cgcgcgccgg agtcggcgca atggccgtcg caggacgagg
     1321 tctgcagccc gccgccgccg ccgcacgacg cgagcagcgc cgcgcaggct agccacgcgc
     1381 cgcgagcgcg catcagcgca ctcccttgcg cgcaggcgtc atcagctgcg tgcgcgcgat
     1441 atggaagccg tggcaagagg tgcaggtgga tggcacgcgt tccggctggc ccggatcgcc
     1501 gtgacagccg cggcagctgg cgatgtcggg cagcagcacg tcgctggaat tcgacgaggc
     1561 gcgcgcggcg tggcagctct cgcatttctg catgcggtgc gcggcgtgat cgaatttcgc
     1621 atgcggatag ccggccagcc agggcgcgac cggcgcgatg cgccacggca gctgcgcgtc
     1681 gccggtgcgt tccaccaggt ggcaatacac gcacacgcgg cggtcgaaca catcgtcgat
     1741 ggcctggcgc gcgcggcgct cggcccaggc caggtcggcg ggcgagggcg tttcgccggg
     1801 tccgggcacg cggcgcagtt cgccgtcgtc atgccgctgc ggatcgccgc cgtgcagcgc
     1861 gcgctgcgcg tagtagtcgc ggatcacctg caccacttcc tgcggtttgg cgtgcggcag
     1921 ctcgcgcgtg ggcgcggccg gatcgaagtc cagcttgtgg cagccgctgc aatgcgcctg
     1981 catgctgacc ggcgcaaaat tcttgccgct gctgtcgggc tggtggcaat cggcgcaatg
     2041 cagctgcttg cgcccctgcg ggctgtcgat gccggaggca agcaggtggc ggtcgtgcgg
     2101 gaagcgcagg ttgagctgct gctgccagtc ggcgctgccg ggttccaccc gcatgatggc
     2161 gaacgtgccg cccgaataac gcgccacgct ggcccgcagc ggcggatggc tgcgcatgaa
     2221 atccgccacc atcggtacac gcgtgtcggt aagaccggct tccggctgac catggcaatc
     2281 ggtgcagatg gccggatgcg gcagcagcag tccgtgatcg ccgtgatgct cgtagtggca
     2341 ggcggcgcag cgtgccttgg cgaactgcac ctgtccgagc agcgcgggat tgtccgtgtg
     2401 ctggtgcacc ccggcgtggc aggcggcgca ggcgctgttg cgcacgcgct cgaacggcgc
     2461 gccatggcag gcgctgcagt tgtcgccgaa ctgcgcgtgg gcgcggctca gcggtccggg
     2521 cagccagacc tggtcgcccg gatcagctgc gttcacgtgg tagcgcagcc acaccggcag
     2581 cagcagtccc ggcagcagca ccgccagcgc cagccaccag gcccagcggc ggcgcgagaa
     2641 gccggtgtgc tccagccgcg tctgcagcgc gcgcaggcgc tgctgcttct cctcgcgcgc
     2701 gctgatccat tcctgcaccg agatcagcag gtcggtgccc agctcgggcg acagcacggt
     2761 gagccggtag cggccgatgt cgatgacgtc gccgatgttg agatcggcat cgtgcagcgg
     2821 cgcgtcgttg acccacacgg tggcgccggg ccgcgccgtg agcttgtacg gccggccggc
     2881 gacgatctcg gcatgcgcca gcgccacgcg gatgtcgccc agttccacgt cctgctcggt
     2941 gccgcggccg atgcgcaggc gctcggtgtc gagcgtgcgc tcggtccaca ccacctggtt
     3001 gccgttcatc gaaagggtgc ggacgacgac gcgcatggct taccagtaga gaaacacgga
     3061 gacgatgtgc gcggcgagcg cggccagcag cgcgaaggtg agcggcacgt gcaggtagag
     3121 ccacacctgc atgcgcgcgg tgagcgtgat gtcgcggttg gcgcgcgcca ccagttcctg
     3181 ccgccgcgcc agcaggtcga gcagcttgcg gatgcggtcg gcctcgctgt tgcccttggc
     3241 gggtgctttc gccggagcct cgtcgaggcc ggcgaccagc tggtcggcca tgaacatcac
     3301 cgtgctctgg ctgctggagg cgaagtcgcc caggcccggc ttgctgaccg gcgcgcgcgc
     3361 cgcctgcatg cgttcggaca ggttctggtt gagcatttcc gccgccgcgg ccgcgcgcgg
     3421 gcgcgtgcgc ccgatcagct gctgccacgc gctgccgccc acgcgcacgc gctcgttgga
     3481 gcgcagcacg cgcgcgtgca cgtcgggccc gagcgtgtcg gcgatcttga gcgcctggtc
     3541 gttcaggtcc tggatttccg ccagccagga ttcgagcatg gcgtcgccgg agttgcgcgt
     3601 gatcagctcc gggtagcgcg catacgccag cacgccgtag atgccgctgg cgatcaccgc
     3661 cagcatcagc agatagctga gcgtgtgcac gttccagccg aagcggaagc cggtgtgcag
     3721 cgtggcgatg atcagcagcg acaggcccag gtagacatgc gccgacaccc acgccttcac
     3781 cgagccgatg ccgcgcgcgt agcggcgctt gcgcacgccg agccacgcca gccacacgat
     3841 gatcagcgcg cccagcgtgc cgagcgtgta gcccagccag ctgccgccgc tggggccttc
     3901 gggcggatcg tgcgcggcgt aggcaaacaa gccggccacg cacagcagcg ccgccagctt
     3961 gaggaaggcg aactggcggt agctgagaaa gccctgatgc agcgcggcca tcgccggtca
     4021 gctcccgcca ccgcgaaaaa tgcctccacc gcaccgcatt ctgacgtgtt ctattttatc
     4081 cattgattat tatttcataa aataaaatat aacttggttt cccagggtgc ggtcggcgtt
     4141 ttttcactcc acggtctgcg ccttgagcac gttgatgaat tcctccgggc ccacgcggat
     4201 cgcggccccg gtcgggcagg cgcgcacgca gctggggcct tgcggcaggt cgcggcacat
     4261 atcgcacttg gccgcctttt tctgcttgtc gctcttgcct ttcttgtgcg ccgttgcgcg
     4321 gcctggctcg gcgccgccga acatgaagta gcgccacagg ctgggccgcg cgtcggcttc
     4381 cacgcccatc tggatcacgc cgtacgggca gttgcgctgg cagttgccgc agccgatgca
     4441 ggcgtcggtg atgtagacct cgccgttctc gtcgcgcttg atcgcgtcgg gcggacagtc
     4501 cttcatgcaa tgcggatgct cgcagtggcg gcacgaggtg ggcacgtgca gctggtcgaa
     4561 ggtgggcccg gcttcgcggt tcagccgcga cagcccatga tgcgtgtcgg cgcaggcctt
     4621 ctcgcagttg tcgcagcgga tgcacagcga ctcgtcgatc agcagcacgt cggtcgcttc
     4681 gccgaagccc tgcgccatga ggaagttgat cacgccgccg aactgcggcg tctcctgcat
     4741 cgccacgttc tgcgccagcc gctcgcggta aagctgtgcg atcagttcct cgatctgcgg
     4801 gtgccgctgc atcagcgcct tgaacgcctc cccctcgacg cggatggttt ccgtggccac
     4861 cgccgcgcgc gccgtggccg agcgcggctg cgacgacacc agcgccatct cgcccacgta
     4921 ttgcccggcc ggcacgtagg ccagcaccac ctcgcgcccg cccagcatgc gcgatacggt
     4981 gaccgagcct ttcctgatca ggtgcagcgc atcgccggct tcgccttcgc ggaacagcac
     5041 gtcgccggcg ttgaactggc acacgcgcgc gctggccagc acctgcgcca ggtcctgcgt
     5101 ggacacgccg ggtgcgatgc gcgcctggat ggcgcggcgc aggaacgcct cgtccaccac
     5161 gcgcttgacc gcggccaccg aggcgatcag cttgctcatc gagcggcgcg gcgtttccag
     5221 cagcacgcag tccgcgccgg cgcgcacggt ggcggtgcgg cggcggccgg agatcaggcc
     5281 gatctcgccg aagaattcgc cggcgccgat gctgatgcgg cggccgctct cgccgctggt
     5341 gtccacttcc accgagccgt cgatcaccga gtagaaactg ttgctgtagt cgttgcgctc
     5401 gaagatcacc gtgcccggcg ccggcgcgtg cagggtggag tcgagcagga actcgcgcag
     5461 ctgcagcggc gtgagcggcg ccagcaccgg cacgcgctgc cggatgcgcg cgatggcttc
     5521 atccaccgag ccccactgcg gcacggcggc gaacttctcg cgcagcaggc cggcgtcggc
     5581 cggttccacc gcgcggccct cgatgaactc caccacctcg tagccctggt tcatcgcctg
     5641 cttgatcagc gggaagccgg ccagcgcgcc gatgatgtac aggcccggca cgttggattc
     5701 gtaggtgctc gacaccgccg gcaccgcgtt gggatcggcg ctggggaagg tgatgccggc
     5761 gctctccacg aacttgcgcg gcggcatggc gcccagccgc gcgatgacgc ggtcgcaagg
     5821 cagttcgctc ttgccggccg cggtgtttac cagcatggcc ccgcgcggct tgccggcgcg
     5881 tgcctgcacc tcggccacgc gcgtgttgta caggcattgc acggtgccat cggcaatggc
     5941 tttcaggatc gccgtgcggt tgccttcctt ggcgcggtcg aagtcgtcgc gctggttgag
     6001 gatggttacc tggttgcgct cggccagcgc cagcgcgttc tccagcccgg cgtcgcccac
     6061 gccgacgacg atgatgcgct cgccggcgta ttcgtcggga tcgtccagct ggtactgcac
     6121 gaagggcagg tcttcgccgg gcacgccgag cttgcggatg ttgccctgca ggccgaccgc
     6181 cagcaccacg aactgcgcgc tcacctgcga cttgtcgccc agggtcagca cgaaggcgcc
     6241 ttgcccgccg gtgatggcga tgacttccgc gcggtggcgc acgttgacct ggtgctgcgc
     6301 cacgccgtcg ttccacgcgc cgagcacttc ctcgcggcgg ccggcggcga agggcagggg
     6361 cgagcgcagc ggcagcacac cgggctcggc catcacgtgc ttgcctttct ggtagcggaa
     6421 gatggtgttg gaaacgtgcg gcgccgcttc cagcagcacg tggctgacgc ccagttcagc
     6481 cgcatgcgca gccgccgaca acccggcggg gccggaaccc acgaccgcga ttttgtagtg
     6541 gtcca
//
LOCUS       DASZZC010000199         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_724555,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000199 DASZZC010000000
VERSION     DASZZC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724555"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="gmhB"
                     /locus_tag="VHE37_04340"
     CDS             <1..534
                     /gene="gmhB"
                     /locus_tag="VHE37_04340"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HVT34782.1"
                     /translation="DRDGVINHDSEEFVKSLAEWRPVEGSLEAIARLCHAGYRVFIAS
                     NQSGLGRGLYDYDTFSAINDRLQKLVGELGGRIDGIEFAPDHPDRATDKRKPAPGMLK
                     DLAKRLQVSLKDVPYVGDSPGDIEAARAAGARPILVRTGKGRRTEMKRDADVAVYDDL
                     AAFVTALLAPPTKPETA"
     gene            complement(638..1012)
                     /gene="folB"
                     /locus_tag="VHE37_04345"
     CDS             complement(638..1012)
                     /gene="folB"
                     /locus_tag="VHE37_04345"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HVT34783.1"
                     /translation="MDHIFIDGLQVHSIVGVHAHEQHAPRLLLLDLQLGCDARRAAAS
                     DQLRDTIDYQAVADALAQLGRSRNFQLIETFAESAARLLFGEFPIATLELTVHKPGAV
                     AGVQAVGVRIKRVREDYAVCGR"
     gene            complement(1012..2022)
                     /gene="tsaD"
                     /locus_tag="VHE37_04350"
     CDS             complement(1012..2022)
                     /gene="tsaD"
                     /locus_tag="VHE37_04350"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HVT34784.1"
                     /translation="MHVLGIETSCDETAVAVYHRGRGLLAHVLHSQIALHAQYGGVVP
                     ELASRDHVARINGLVRSCLEKAGMAAQDLDGIAYTAGPGLIGALMVGASVAAGMAAAL
                     DIPLVPVHHMEAHLLAPMLETPAPEFPFIALLVSGGHTQLVAVRGIGQYELLGESLDD
                     AAGEAFDKTAKLLGLPYPGGPHLARLAQQGHSGVYKFPRPMTDRPGLDFSFSGLKTSV
                     LTALKADADDQDKADLARGFEDAVTQTLAIKCERALEQTGFERLIIAGGVGANQRLRE
                     LLAQLARGRGAQVYFPRNEFCTDNGAMIAYAGCARLAQGSREPQTLALKPRWPLTELA
                     PV"
     gene            2115..2330
                     /gene="rpsU"
                     /locus_tag="VHE37_04355"
     CDS             2115..2330
                     /gene="rpsU"
                     /locus_tag="VHE37_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HVT34785.1"
                     /translation="MPNVRVRENEPFEVALRRFKRTCEKAGVLTDVRKHEYFEKPSQE
                     AKRKRAAAVKRQAKKVSRETTRRTRLY"
     gene            2350..2796
                     /locus_tag="VHE37_04360"
     CDS             2350..2796
                     /locus_tag="VHE37_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HVT34786.1"
                     /translation="MSDLKTRINDDVKSAMKAGDKARLSVLRMLTAELKQREVVDNVE
                     LTEGVIHASLEKMIKQRRDSEKQFRDGGRAELADKEAAEIQVLLSYLPQQLSDAEIAA
                     LVAEAIAATGAAGGKDMGKVMGWIKPKVAGRADMGKVSALIKSKLG"
     gene            2823..>4364
                     /gene="dnaG"
                     /locus_tag="VHE37_04365"
     CDS             2823..>4364
                     /gene="dnaG"
                     /locus_tag="VHE37_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HVT34787.1"
                     /translation="MAGGRIPEAFIHDLLARVDIVELIGSRVELKRAGKEYKARSPFS
                     NEKTPSFFVSPVKQMFFDFSSGKNGNAIGFLMDYDRLSFVEAVEELARRAGVEVPRDG
                     GHGPDRLVLEGPLDALAAAQRWFREQLKTAPQAIEYLKKRGVNGETAKTYGIGWAPES
                     FDALTKFFDAPEHALAAGLLKKKEETGRVYDGFRARVMFPIRDTRGRVIAFGGRAMGD
                     EKPKYLNSPETALFHKGRNLYGLFEARQSSKSALPYLVVVEGYMDVVMLAQHGIREVV
                     GTLGTATTRDHLNLLYKSTSRVVFCFDGDRAGRSAAWRALEQALPEIYDQREAAFMFL
                     PEGHDPDTLVQEIGADKFRELIAQAMSLSQFLLSELSKQVNLGTLDGRARLASLAKPY
                     VQKLREGPLRSLVLDELAKITRLSVAQLQSEAPLGVREPAPGNARAAARTESAAARPV
                     KRALQLLLEKPEMADEVQNLELLAQADVPGIAVLIDALDWLHANPGARAAQLVAAWHE
                     TPKGAA"
BASE COUNT          771 a         1441 c         1468 g          684 t
ORIGIN      
        1 gaccgcgacg gcgtgatcaa ccacgactcc gaggagttcg tgaaatcgct ggccgagtgg
       61 cgcccggtgg agggcagcct ggaggccatc gcgcgcctgt gccacgccgg ctaccgcgtg
      121 ttcatcgcgt cgaaccagtc ggggctcggc cgcgggctgt acgactacga caccttcagc
      181 gccatcaacg accgcctgca gaaattggtg ggcgaactcg gcgggcgcat cgacggcatc
      241 gagttcgcgc ccgatcatcc agaccgtgcc accgacaagc gcaagccggc gccgggcatg
      301 ctcaaggatc tggccaagcg cctgcaggtc tcgctcaagg acgtgcccta cgtgggcgat
      361 tcgcccggcg atatcgaggc ggcgcgcgcg gcgggcgcgc gcccgatcct ggtgcgcacc
      421 ggcaaggggc gtcgcactga aatgaagcgc gatgccgatg tcgcggtgta tgacgacctt
      481 gccgccttcg tcaccgcact gctcgcgcct cccacaaagc ccgaaaccgc ctgaccgcac
      541 tgccgcttta cgtgttataa aatataatct gatttaaata taatataata attaataaca
      601 tgaatgcagg cagtgcggtg gccgtgtttt ttccgcttca acgaccgcag acggcatagt
      661 cctcgcgcac acgcttgatg cgcacgccca ccgcttgcac gccggcgacc gcgcccggct
      721 tgtgtacggt gagctccagc gtggcgatgg gaaattcacc gaacagcagg cgcgctgcgc
      781 tttcggcgaa ggtttcgatc agctggaaat tgcgcgagcg tccgagctgc gcgagcgcat
      841 cggccaccgc ctggtagtcg atggtgtcgc gcagctggtc gctggcggcg gcgcggcgcg
      901 catcgcagcc cagctgcaga tcgagcagca acaggcgcgg cgcgtgctgc tcgtgcgcat
      961 gcacgccgac gatgctgtgc acctgcaggc cgtcgatgaa aatgtgatcc atcagacggg
     1021 cgccagttcg gtcagcggcc agcgcggctt gagcgccagc gtctgcggtt cgcgcgagcc
     1081 ctgcgccagc cgcgcacagc cggcgtacgc gatcatcgcg ccgttgtcgg tgcagaactc
     1141 gttgcgcgga aagtacacct gcgcgccgcg cccgcgcgcg agctgcgcca gcaattcgcg
     1201 caggcgctgg ttggcgccga cgccgccggc gatgatcagc cgctcgaagc cggtctgctc
     1261 cagcgcgcgc tcgcacttga tggccagcgt ctgcgtcacc gcgtcttcga agccgcgcgc
     1321 gagatcggcc ttgtcctggt cgtcggcgtc ggccttcagc gcggtgagca ccgaggtctt
     1381 caggccgctg aaggaaaaat ccagcccggg gcgatcggtc atcggccgcg gaaatttata
     1441 aacgccggaa tggccttgct gtgccagccg ggccaggtgc ggcccgcccg gatacggcag
     1501 tcccagcagc ttggcggtct tgtcgaaggc ttcgccggca gcgtcgtcga gactttcccc
     1561 gagcaactcg tactggccga tgccgcgcac cgccaccagc tgcgtgtggc cgccggagac
     1621 cagcagcgcg atgaacggaa actccggcgc cggcgtttcc agcatcggcg ccagcaggtg
     1681 cgcttccatg tgatgcaccg gcaccagcgg gatgtcgagc gccgcggcca tgccggcggc
     1741 gacgctggcg ccgaccatca gcgcgccgat caggcccggg ccagcggtgt aggcgatgcc
     1801 atcgagatcc tgcgcggcca tccccgcctt ttccaggcag ctgcgcacca ggccattgat
     1861 gcgcgccacg tggtcgcgcg aggccagttc cggcaccacg ccgccgtatt gcgcgtgcag
     1921 ggcgatctgg ctgtgcagca cgtgcgccag caggccgcgc ccgcggtggt agacggcgac
     1981 ggcggtttca tcgcaggagg tttcgatgcc caggacgtgc atggcggcgc atattctggc
     2041 cgcgttgcgc gcgcagggcc aatccgccta aaatcgcgcc cctttttggc cctgtgtctg
     2101 tagagagcga ttgaatgcct aacgtccgcg tccgcgaaaa cgaacctttt gaggtggccc
     2161 tgcgccgctt caagcgcacc tgcgaaaaag ccggcgtgct gaccgacgtg cgcaagcacg
     2221 agtacttcga gaagccgagc caggaagcca agcgcaagcg cgcggcggcc gtgaagcgcc
     2281 aggccaagaa ggtttcgcgc gaaaccacgc gccgcacgcg gttgtattga ttccgcactc
     2341 gctcgttcca tgagcgatct gaaaacccgc atcaacgacg acgtgaagtc cgccatgaag
     2401 gcgggcgaca aggcgcgcct gtcggtgctg cgcatgctca ccgccgagct gaagcagcgc
     2461 gaggtggtgg acaacgtcga gctgaccgaa ggcgtgatcc atgcctcgct cgagaagatg
     2521 atcaagcagc gccgcgactc ggaaaagcag ttccgcgacg gcggacgcgc cgagctggcc
     2581 gacaaggaag cagccgagat ccaggtgctg ctgtcctacc tgccgcagca gctatccgac
     2641 gccgagatcg ccgcgctggt cgccgaggcc atcgccgcca ccggcgcggc cggcggtaag
     2701 gacatgggca aggtcatggg ctggatcaag ccgaaagtgg cgggccgcgc cgacatgggc
     2761 aaggtgtccg cgctgatcaa gagcaagctc ggctgaccac agccgcgtct tagaatgccg
     2821 caatggctgg cggcagaatc cccgaagcgt tcatccacga cctgctggcg cgggtcgata
     2881 tcgtcgagct gatcggctcg cgcgtggagc tcaagcgcgc cggcaaggaa tacaaggcgc
     2941 gctcgccgtt ctccaacgag aagacgccgt cgtttttcgt ttctccggtc aagcagatgt
     3001 tcttcgactt ttcctccggc aagaacggca acgccatcgg cttcctgatg gactacgacc
     3061 gcctgagttt cgtcgaggcg gtggaggaac tggcgcgccg cgccggcgtc gaggtgccgc
     3121 gcgacggcgg tcacgggcct gaccggctgg tgctggaagg cccgctcgat gcgctcgccg
     3181 ccgcgcagcg ctggttccgc gagcagctca agaccgcgcc gcaggccatc gagtacctga
     3241 agaagcgcgg cgtcaacggc gagacggcca agacctacgg catcggctgg gcgccggaat
     3301 cgttcgatgc tttgaccaag ttcttcgacg cgccggagca tgcgctggcg gccgggctgc
     3361 tgaagaaaaa ggaggagacc gggcgcgttt acgacggctt ccgcgcacgc gtcatgttcc
     3421 ccatccgcga cacgcgcggg cgcgtcatcg ccttcggcgg ccgcgccatg ggcgatgaaa
     3481 aacccaagta cctcaactcg ccggagaccg cgctgttcca caagggccgg aatctttacg
     3541 gcctgttcga ggcgcggcaa tcgtcaaaat cggcgctgcc gtatcttgtg gtggtcgaag
     3601 gctatatgga cgtggtgatg ctggcgcagc acggcattcg tgaagtggtg ggcacgctgg
     3661 gcaccgccac cacacgcgat cacctgaacc tgctctacaa atccaccagc cgcgtggtgt
     3721 tttgcttcga cggcgaccgc gccgggcgca gcgccgcctg gcgcgcgctg gagcaggcgc
     3781 tgccggaaat ctacgaccag cgcgaagccg ccttcatgtt cctgccggaa ggccacgacc
     3841 ccgacacgct ggtgcaggag atcggcgcgg ataaattccg cgagttgatt gcgcaggcca
     3901 tgtcgctgtc gcagttcctc ctctcggagc tgtccaagca ggtcaacctg ggcacgctcg
     3961 acggccgcgc gcggctggct tcgctggcca aaccttacgt gcagaagctg cgcgagggcc
     4021 cgctgcgcag cctggtgctg gacgaactgg ccaagatcac ccgcctgtcg gtggcccagc
     4081 tgcagagcga agcgccgctg ggcgtgcgcg aaccggcacc cggcaacgca cgcgcagccg
     4141 cgcgcacgga gtcggcagcg gcgcggccgg tgaagcgggc cttgcagctg ctgctggaaa
     4201 aacccgaaat ggccgacgag gtgcagaacc tcgaactgct ggcccaggcc gatgtcccag
     4261 ggatcgccgt gctgatcgat gcgctggact ggcttcatgc caaccctggc gcgcgggcgg
     4321 cgcaattggt ggccgcctgg cacgagacgc ctaagggcgc tgcg
//
LOCUS       DASZZC010000200         5261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1109984,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000200 DASZZC010000000
VERSION     DASZZC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109984"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="VHE37_04370"
     CDS             complement(<1..1765)
                     /locus_tag="VHE37_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34788.1"
                     /translation="MTRERKQLSFREKLQLELGSYLRHEETRFNPADRLKIDFHCHDR
                     NSDVPDELWGRLLGLPETWLETRDLVKCLQGNGCDIITITNHNNARSCWDLLDKGQDV
                     LVGTEFTCHFTEYELYMHVLAYGFTPEQETILNKKRRDIYQFLRYTAEHNIPVILPHP
                     LYFYSRNDKLDPELFEKLAVMFQRYEVLNGQRDLWQSVLVLNWARGLTEEKLHWFSKK
                     HDLDPAGFGVDIARPKILTGGSDDHMGLSAGQSGSYLHVPNLAERRKTAPLSQLALEA
                     IRKGDIAPFGHVGENQRLNVALLDYFSQIVTHMQDPGLLRLMLHRGEASDKLACLVIG
                     NIFLEIKKHKKTRRFISCVHDALRGRKPDWMLRLAVSRDYAFCVKHLEAIADSRHQSP
                     DVSAEQTHKAIGEMYTGLSQLIVERTRKCAAKHGDVFDGDFSSENLIRKFEIPIQIRS
                     LAFGEAPGKSNITGINIGEILDNLSFPVMVNLVLLAAQLASTRVLYANRGFLNEFSDH
                     IGRNPHPRRALYLTDTLRDKNGVSNSLSGKLKEIQRQDYPVDFLICHPDAKPEPHLHV
                     VRPLAEFGFPSFGEQQLRVPDL"
     gene            complement(1767..3494)
                     /locus_tag="VHE37_04375"
     CDS             complement(1767..3494)
                     /locus_tag="VHE37_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT34789.1"
                     /translation="MTSNIAVDVFVVLAGAVLLYRLWVRLALSRAKHPSLRGHARWSR
                     RMARLLSQFDYSGERFFSSDLAPPDVAARRRDGFERLRQQIAGASPQSLHYSQSLEDS
                     ISDVRFTSNYRVPFPYRRDLPREFRFGTMAQETRGRQVCDLDGVWRYDLAGSYGVNVF
                     GYDFYKQCLEAGFARAQELGPVLGPYHPVIRDNVEALKKISGLDEVSFHMSGTEAVMQ
                     AVRLARYHTGRTHLVRLCGAYHGWWDGVQPGVGNTRKVDDVYTLADLSERTLHVLETR
                     NDIACVLINPFQALHPNSDAPGDSALVASDRGTAFDRARYSKWLQRIREVCTRRGVVL
                     IFDEVFAGFRLGHRGAQEYFGVQADLVTYGKTLGGGLPIGVVCGTHALMKRYRDEQPA
                     NISFARGTFNSHPYVMTCMHEFLTRIAQPDVQQIYAEADACWNRRVQQLNDMLGAAQL
                     PVRLSNMHSILSVQYTQPCRYNWMLQFYLRAEGLELSWTGTGRLIMSLDYTDADFAEV
                     AQRLVHGAAQMQRDGWWWSAPHLTNKWIKRQMFKDMLAARFAGRGRGQPASMAEAGRT
                     PGGTLEEVA"
     gene            complement(3754..5139)
                     /locus_tag="VHE37_04380"
     CDS             complement(3754..5139)
                     /locus_tag="VHE37_04380"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HVT34790.1"
                     /translation="MDNDPPDKSSLRRLKTRPLERNIALTRLGLGAGTKIVAHSLLNI
                     FRGEIARDSADRAFYERQARILADELGQLKGSVMKAGQMLSLYGQYFLPPEAVAVLSQ
                     LQDTTPPVHWSVVAPVLRRALGTARLNELEIEHEPIAAASLGQAHRARRKSDGLALCV
                     KVQYPGVADAIESDISTLSRLLLMTRLAPKGLDLEPVFNEVREMLHREVDYESERHFT
                     EEFAQRLSADARFVVPRVVPEYCSDMVLTTTFEEGVHVRDAAVQALSQERRNALGRAF
                     LELFLREFFDWGMVQSDPHFGNYRFRLDAKKGDRIVLLDFGATRVFGRGFIDAYADIV
                     RGALLGDADGVERGAQAIGLMRPGMPRSVLDAYAQLCTLIVEPFSPDHAPPALLTANG
                     AYRWGESDLPMRAGSLAARNALSVHFRVPPREIVFLHRRLAGVFIMLATLRCEIATRD
                     LLLQFLERAGS"
BASE COUNT          856 a         1716 c         1793 g          896 t
ORIGIN      
        1 tcaggtccgg cacgcgcagc tgctgctcgc cgaagctggg gaagccgaac tcggccagcg
       61 ggcgcaccac gtgcaggtgc ggctcgggct tggcatcggg gtggcagatg aggaaatcca
      121 ccgggtaatc ctggcgctgg atttccttga gcttgcccga cagcgaattc gacacgccgt
      181 tcttgtcgcg cagcgtgtcg gtgagataca gcgcgcggcg cggatgcggg ttgcggccga
      241 tatggtccga gaactcgttg aggaagccgc ggttggcata gagcacgcgc gtgctggcca
      301 gctgtgccgc cagcaggacc aggttcacca ttaccgggaa cgagagattg tcgaggatct
      361 cgccgatgtt gatgccggtg atgttgctct tgcccggcgc ttcgccgaag gccagcgaac
      421 ggatctggat gggaatctcg aacttgcgga tcaggttctc gctggagaaa tcgccgtcga
      481 acacatcgcc gtgcttggcc gcgcatttcc tggtgcgctc gacgatcagc tgcgacaggc
      541 cggtgtacat ctcgccgatg gccttgtgcg tctgctcggc ggacacgtcc ggcgactggt
      601 ggcggctgtc ggcgatcgct tcgaggtgct tgacgcagaa cgcgtagtcg cgcgacaccg
      661 ccaggcgcag catccagtcg ggcttcctgc cgcgcagcgc gtcgtgcacg cagctgatga
      721 agcgcctggt cttcttgtgc ttcttgatct cgaggaagat gttgccgatg accaggcagg
      781 cgagcttgtc gctggcctcg ccgcggtgga gcatcaggcg cagcaggccc gggtcctgca
      841 tgtgcgtgac gatctgcgag aaatagtcga gcagcgccac gttgagccgc tggttctcgc
      901 cgacatggcc gaacggcgca atgtcgccct tgcggatcgc ttcgagcgcc agctgcgaca
      961 gcggcgcggt cttgcgccgc tccgccaggt tgggcacgtg caggtaggag cccgactgcc
     1021 cggccgacag gcccatgtgg tcgtccgagc cgccggtgag gatcttcggc cgcgcgatgt
     1081 cgacgccgaa cccggcgggg tcgaggtcgt gcttcttcga gaaccagtgc agcttttcct
     1141 cggtgagtcc gcgcgcccag ttgagcacca gcacgctctg ccacaggtcg cgctggccgt
     1201 tgagcacttc atagcgctgg aacatcactg ccagcttctc gaacagctcc gggtcgagct
     1261 tgtcgttgcg cgagtagaaa tacagcgggt gcggcaggat caccgggatg ttgtgctccg
     1321 cggtgtaacg caggaactgg tagatgtcgc gccgcttctt gttgaggatg gtttcctgct
     1381 ccggcgtgaa gccgtacgcg agcacgtgca tgtacagctc gtactcggtg aagtggcagg
     1441 tgaactcggt gccgaccagc acgtcctggc ctttgtccag caggtcccag caggagcgcg
     1501 cgttgttgtg gttggtgatg gtgatgatgt cgcagccgtt gccctgcagg cacttcacca
     1561 ggtccctggt ttccagccag gtctcgggca ggccgagcag gcgtccccac agctcgtcgg
     1621 gcacgtcgct gttgcggtcg tggcagtgga agtcgatctt caggcggtcc gccgggttga
     1681 agcgggtttc ctcgtgccgc agatagctgc ccagttccag ctgcagcttc tcgcggaagg
     1741 aaagctgttt gcgttcgcgc gtcatgtcag gccacctctt ccaaggtccc gcccggggtc
     1801 cggcccgctt cggccattga cgccggctgt ccgcggcccc gtcccgcgaa acgcgccgcc
     1861 agcatgtcct tgaacatctg ccgcttgatc cacttgttgg tcaggtgcgg cgcgctccac
     1921 caccagccgt cgcgctgcat ctgcgccgcc ccatgcacca agcgctgcgc cacctcggcg
     1981 aaatccgcat cggtgtagtc gaggctcatg atcagccggc cggtgccggt ccacgacagc
     2041 tcgagcccct cggcgcgcag gtagaactgc agcatccagt tgtagcggca cggctgcgtg
     2101 tactgcaccg acaggatgct gtgcatgttg gacagccgca ccggcagttg cgcggcgccg
     2161 agcatgtcgt tgagctgctg cacgcggcgg ttccagcagg cgtcggcttc ggcgtagatc
     2221 tgctgcacgt ccggctgcgc gatgcgcgtg aggaactcgt gcatgcaggt catcacgtac
     2281 ggatgcgagt tgaaggtgcc gcgcgcgaac gagatgttgg ccggctgctc gtccctgtag
     2341 cgcttcatca gcgcatgggt gccgcagacc acgccgatcg gcaggccgcc gccgagcgtc
     2401 ttgccgtagg tgaccaggtc ggcctgcacg ccgaaatatt cctgcgcgcc gcggtggccg
     2461 agccggaaac cggcgaacac ctcgtcgaag atcagcacga cgccgcggcg cgtgcagacc
     2521 tcgcggatgc gctgcagcca cttgctgtag cgcgcgcggt cgaacgcggt gccgcgatcg
     2581 ctggccacca gcgccgaatc accgggcgca tcgctgttcg ggtgcagcgc ctggaacgga
     2641 ttgatcagca cgcaggcgat gtcattgcgc gtttccagca cgtgcagcgt gcgctccgac
     2701 aggtcggcca gcgtgtagac gtcgtcaacc ttgcgcgtgt tgcccacgcc cggctgcacg
     2761 ccgtcccacc agccgtgata ggcgccgcac aggcgcacca ggtgcgtgcg gccggtgtgg
     2821 tagcgcgcca gccgcaccgc ctgcatcacc gcctcggtgc cggacatgtg gaacgacact
     2881 tcgtccaggc cggaaatctt cttcagcgcc tcgacgttgt cgcggatcac cgggtgatac
     2941 gggccgagca ccgggcccag ttcctgcgcg cgcgcgaagc cggcttcgag acattgcttg
     3001 tagaagtcgt agccgaacac gttgacgccg taggagccgg cgaggtcgta gcgccacacg
     3061 ccatcgaggt cgcagacctg ccgcccgcgg gtttcctgcg ccatcgtgcc gaagcggaac
     3121 tcgcgcggca ggtcgcgccg gtacgggaac gggacgcggt agttgctggt gaagcgcacg
     3181 tcggaaatgc tgtcttccag cgactggctg tagtgcagcg actgcggtga agcgccggcg
     3241 atctgctggc gcagccgctc gaatccgtcg cgccggcgcg cggccacgtc gggcggcgcc
     3301 aggtcgctgc tgaagaaacg ctcgccgctg taatcgaact gcgacagcag ccgcgccatg
     3361 cggcgcgacc agcgcgcgtg tccgcgcagc gaaggatgct tggcccgtga aagggcgagc
     3421 cggacccaga gccggtacag cagcaccgca ccagccagga ccacgaagac gtcgacagcg
     3481 atattggaag tcacgttgca agaatgctgg ccgtgcaaga cggcaatgtg acaatcgatt
     3541 gaagattaca tgtcgccgga cctggcccgg ccgccgaaaa acggcttgcg gtgggcaccc
     3601 cgcgcaggcg ttttacccgc ggcgggcgac cggaatattt cagcgtcgat cctcgatgct
     3661 gtcctgcccg ttcatgcgcg tcccccgacg tgatgtgcgc gcaaccttag cgcaggcgcg
     3721 caggcccgcg cacctgtccg tacgaccagg ggttcagctc ccggcgcgtt cgaggaactg
     3781 cagcagcagg tcgcgcgtgg cgatctcgca gcgcaatgtg gccagcatga tgaacacgcc
     3841 cgccaggcgc cggtgcagga acacgatctc gcgcggcggc acgcggaaat gcaccgacag
     3901 cgcgttgcgc gcggccagcg agccggcgcg catcggcagg tcggattcgc cccagcgata
     3961 ggcgccgttt gcggtcagca gtgccggtgg cgcgtggtcc ggcgagaacg gctcgacgat
     4021 cagcgtgcac agctgcgcgt aggcatcgag cacgctgcgc ggcatacccg gccgcatcag
     4081 gccgatggcc tgcgcgccgc gctcgacgcc atcggcatcg cccagcagcg cgccacgcac
     4141 gatgtcggcg taggcgtcga tgaagccgcg cccgaacacg cgggtggcgc cgaaatcgag
     4201 cagcacgatg cggtcgccct tcttcgcatc gagccggaaa cggtaattgc cgaagtgcgg
     4261 atcggactgc accatgcccc agtcgaagaa ttcgcgcagg aacagctcga ggaacgcgcg
     4321 gcccagcgcg ttgcgccgct cctgcgacag cgcctgcacc gcggcatcgc gcacgtggac
     4381 gccttcctcg aaggtggtgg tcagcaccat gtcgctgcag tattccggca ccacgcgcgg
     4441 taccacgaag cgcgcatcgg ccgacagccg ctgcgcgaat tcctcggtga agtggcgctc
     4501 gctttcgtag tccacttcgc ggtgcagcat ctcgcgcacc tcgttgaaca ccggctcgag
     4561 atcgagcccc ttgggcgcca gccgcgtcat cagcagcagg cgcgacagcg tggagatgtc
     4621 gctctcgatg gcgtccgcca cgccgggata ctgcaccttc acgcacagtg ccaggccgtc
     4681 gctcttgcgc cgcgcgcggt gcgcctggcc cagcgaggca gcagcgatgg gttcgtgctc
     4741 gatctccagc tcgttgagcc gcgccgtgcc cagcgcgcgg cgcagcaccg gcgccaccac
     4801 gctccagtgc accggcggcg tggtgtcctg cagctggctg agcaccgcca cggcttccgg
     4861 cggcaggaag tactggccgt acagcgacag catctgcccg gccttcatca cgctgccctt
     4921 gagctggccc agttcgtccg cgaggatgcg cgcctgccgc tcgtagaacg cgcggtcggc
     4981 cgaatcgcgc gcgatctcgc cgcggaagat gttgagcagg ctgtgcgcga caatcttggt
     5041 gccggcgccc aggcccaggc gcgtaagcgc gatgttgcgc tccagcgggc gggtcttcag
     5101 ccggcgcagg ctggatttgt ccggtgggtc gttgtccaat gtgcgggtcg ataatcagga
     5161 cggggagaga gcatcgatgc agacgctcaa cgcaattcta ctggctgacc ataagaagca
     5221 gcaggtgcag cgcgagttca ctcagctgat cgaggacttc g
//
LOCUS       DASZZC010000201         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_18196,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000201 DASZZC010000000
VERSION     DASZZC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18196"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VHE37_04385"
     CDS             complement(<1..1084)
                     /locus_tag="VHE37_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HVT34791.1"
                     /translation="MALATVLSRAQNGLSADLVTVEVHLAPGLPGLNIVGLPEAAVRE
                     SKDRVKAAITTCGYQFPNRRITCNLAPADLPKDGARFDLAIALGILAASGQVPAEALQ
                     DVEVLGELSLSGELRPVQGVLLASLKATAAGRRLLVPEANAAEALLAGNAQAVAAKHL
                     AALCTALHANQLAAAHVPEVPQVVQSGPDLAEVRGQAQAKRVLEIAAAGGHSLLMMGP
                     PGSGKSMLAQRLPGILPPLTDEEALEVAAVASIGQEGFDVSRHWRRRPYRAPHHTASG
                     VALVGGGASPRPGEITLAHLGVLFLDELPEFDRRVLEVLREPLESGSITVSRAARQAD
                     FPARFQLVAAMNPCPCGYLGDSSAKCT"
     gene            complement(1088..1327)
                     /locus_tag="VHE37_04390"
     CDS             complement(1088..1327)
                     /locus_tag="VHE37_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HVT34792.1"
                     /translation="MLDNKQIEDLAAKLGRVIPPGLKGLRQELEDNFRAVLRANLEKL
                     DLVSRERFEIQAELLARTQARLAALEARLQSLEKN"
     gene            1518..1856
                     /gene="glnK"
                     /locus_tag="VHE37_04395"
     CDS             1518..1856
                     /gene="glnK"
                     /locus_tag="VHE37_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVT34793.1"
                     /translation="MKLISAIIKPFKLDEVREALSDLGVAGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVAVDDDKCDPAIDAISKAAHTGKIGDGKIFVFSLDQAVRI
                     RTGETGKDAL"
BASE COUNT          352 a          677 c          659 g          332 t
ORIGIN      
        1 aagtgcactt ggctgagctg tcgccgaggt agccgcacgg acatggattc atcgccgcca
       61 ccagctggaa gcgcgccgga aagtccgcct gccgggccgc acgcgaaacg gtgatgctgc
      121 cgctttccag gggctcgcgc agtacttcca gcacgcgtcg atcgaattcc ggcagttcgt
      181 cgaggaacag tacgcccagg tgcgccagcg tgatttcgcc aggtctgggc gacgcgcccc
      241 ctccgaccag cgccacaccg gaagccgtgt gatgcggtgc gcggtagggt cggcggcgcc
      301 agtgccggct cacgtcgaaa ccttcctgcc cgatggaagc cactgcggct acttcgagcg
      361 cttcctcatc ggtcagcggc ggaagtattc caggcaggcg ttgcgccagc atggatttgc
      421 ctgatcccgg cggccccatc atcagcagtg aatgcccgcc ggccgcggcg atttcgagca
      481 cacgcttggc ctgtgcctgg ccgcgcactt cggcaaggtc cggcccggac tgcaccactt
      541 gcggcacctc cggcacgtgg gccgcggcga gttggttggc gtgcagtgcc gtgcaaagag
      601 cggcaaggtg tttcgctgca acggcctgtg cgttgccggc cagcaaggcc tcggccgcat
      661 tggcttccgg caccagcaga cggcgcccgg cggcggtggc cttgagcgat gccagcagca
      721 cgccctgcac ggggcgcagt tctcccgaca acgacagttc gccgagcacc tcaacgtcct
      781 gcagcgcttc ggcgggcacc tgcccggacg ccgccagtat tccgagcgcg atggccagat
      841 cgaagcgcgc gccatccttg ggcaggtccg ctggggcgag gttgcaggtg atgcggcgat
      901 tgggaaactg gtagccgcag gtggtgatgg ccgccttcac ccgatccttg gattcgcgca
      961 ccgccgcttc ggggaggcct acgatattga ggccaggcag gccaggcgcg agatgcactt
     1021 ccaccgtcac caggtcggcc gaaaggccgt tctgggcgcg cgacagcacg gtggccagcg
     1081 ccatgcgcta gttcttttcc agcgactgca ggcgcgcttc cagcgctgcc agccgcgcct
     1141 gcgtgcgcgc gagcagttcc gcctggatct cgaaacgctc gcgcgaaacc agatccagct
     1201 tttcgagatt ggcccgcagc accgcacgga agttgtcctc gagttcctgc cgcagtccct
     1261 tcaggccggg tggaatcacg cgtccgagct tggcggcgag gtcttcgatc tgcttgttgt
     1321 ccagcatgcg gcccatcccc ggaaagtcgc gcgagtttat cgcgcgtgag cctgcatcgc
     1381 gcgcacccaa atagtgcgac agcgctggat gctgccccaa aaaacgctgg catgctccgt
     1441 gcattctcgc ccgaggccgc gggggaaaag gctgcccacg cgtatgtcgc ccatatgcat
     1501 acaccaggag tgatttcatg aaactgattt cagccatcat caagccgttc aagctggacg
     1561 aagtgcgtga agcgctctcg gatctcggcg tggccggcat cacggtgacg gaagtgaaag
     1621 gcttcggccg ccagaaaggc cataccgagc tgtaccgcgg cgccgagtac gtggtcgatt
     1681 tcctgccgaa ggtgaagatc gaggtggcgg tcgacgacga caagtgcgac cccgccatcg
     1741 acgccatcag caaggccgcg cataccggca agatcggcga cggcaagatc ttcgttttct
     1801 cactcgacca ggctgtccgt atccgtacgg gcgagaccgg caaagacgcg ctctaaggga
     1861 gagccctcaa tgatccgaca actttcgata gcactgctgt tcgccgcctc gctcggcttc
     1921 gcgccggcgt gggccgacga tgccgccccg gcgcccgcgc cggaagcagc agcagccccg
     1981 gccgaagccg cgcccgccgc ggcgccgacg gccgaaaaaa
//
LOCUS       DASZZC010000202         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1172901,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000202 DASZZC010000000
VERSION     DASZZC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172901"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /gene="gcvPB"
                     /locus_tag="VHE37_04400"
     CDS             <1..1465
                     /gene="gcvPB"
                     /locus_tag="VHE37_04400"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HVT34794.1"
                     /translation="NMSEKLIFEVSRPGRSAAAQNPGHAIDLSGVPQHLRRKNPPLLP
                     EVSELQAVRHYTRLSQLNFSIDTHFYPLGSCTMKYNPRACNTLAMLPEFLGRHPLAPA
                     THSQGFLSCMYELQEILKEVTGMAGVSLTPMAGAQGEFAGVAMMLAYHKREGDHKRTE
                     ILVPDAAHGTNPATAAMLGCTVKEIPTDDHGDVDVEALKAAVGPQTAGIMLTNPSTLG
                     VFERRIKEIAGIVHQAGGLLYYDGANLNAILGKVRPGDMGFDVIHMNLHKTFSTPHGG
                     GGPGAGAVGVSERLKPFMPIPIVGREGDQFRWFDERDVPETIGRLSAFMGNAGVLLRA
                     YVYARMLGREGMHRVAEFAALNANYLAAALKAKGFDLAFDGRRASHEFIITLKKQKQE
                     IELTATDVAKRLLDYGFHAPTIYFPLLVPECLLIEPTETEDKETLDAFVGALVSIWDE
                     AKKDVQYVKGAPYTMPVRRLDDVRAARQLDLRYQASR"
     gene            1471..1830
                     /locus_tag="VHE37_04405"
     CDS             1471..1830
                     /locus_tag="VHE37_04405"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HVT34795.1"
                     /translation="MADGLKGWKRLWRATVVSYWGLGYALRNEEAFRIEFALCVLLAP
                     LALWLGHNKVERIIMLMSLFVVLITELLNSGIEVAIDRISKEQHPLSGAAKDLGSAAV
                     HLSLLQVPLVWSLVLFT"
     gene            1853..2785
                     /locus_tag="VHE37_04410"
     CDS             1853..2785
                     /locus_tag="VHE37_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HVT34796.1"
                     /translation="MSALICGSFAFDTIMVFPGQFKNEILPDKVHMLNVSFLVPELRR
                     EFGGTAGNIAYNLNLLGGSVLPMGTVGDDFDNYAAWMDQCGISRKYVKQVAGTYTAQA
                     YITTDLDDNQITAFHPGAMNHAHTQEIPAKGIKIGSISPDGRDGMIQHAAQFADAGVP
                     FLFDPGQGLPMFTGAELRSFIQQASYCAVNDYESELLMERTGWKLKQIAERLDALIVT
                     RGGKGSHIITKKKTFEIPVAQASALADPTGCGDAYRGGLLYGLQKGMDWETTGRIAAL
                     MGAINIERPGTQNHKFTLEQFRARFKKEFRYELR"
     gene            2788..>2959
                     /locus_tag="VHE37_04415"
     CDS             2788..>2959
                     /locus_tag="VHE37_04415"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HVT34797.1"
                     /translation="MLTLKLKSHEERRLRAGHLWAYSNEIETDAGWKNIAPGTLCRLN
                     DSRGQPLGIGYVN"
BASE COUNT          552 a          978 c          948 g          481 t
ORIGIN      
        1 caacatgagc gagaagctga tttttgaagt gtcgcgtccg ggccgcagcg cggccgcgca
       61 gaatccgggc catgcgatcg atctctcggg cgtgccgcag cacctgcgcc gcaaaaaccc
      121 gccgctgctt cccgaggtgt cggagctgca ggcggtgcgc cattacacgc gcctgtcgca
      181 gctgaacttc tccatcgaca cgcacttcta cccgctgggt tcgtgcacga tgaaatacaa
      241 cccgcgtgcc tgcaacacgc tggccatgct gccggaattt ctcgggcgcc atccactggc
      301 gccggccacg cacagccagg gtttcctctc gtgcatgtac gagctgcagg aaatcctcaa
      361 agaggtgacc ggcatggccg gcgtgtcgct gacgccgatg gccggcgcgc agggcgagtt
      421 cgccggcgtg gccatgatgc tcgcgtacca caagcgcgag ggcgaccata agcgcaccga
      481 gatcctggtt cccgatgcgg cgcatggcac caacccggcg accgccgcca tgctcggctg
      541 cacggtgaag gagatcccca ccgacgatca cggcgacgtc gatgtggagg cgctcaaggc
      601 cgcggtcggt ccgcagaccg cgggcatcat gctgaccaat ccgtcgacgc tgggcgtgtt
      661 cgagcggcgc atcaaggaga tcgccggcat cgtccaccag gccggcggcc tgctgtacta
      721 cgacggcgcc aacctcaacg cgatcctggg caaggtgcgc ccgggcgaca tgggcttcga
      781 cgtcatccac atgaacctgc acaagacctt ctccacgccg cacggcggcg gtggtccggg
      841 cgcgggcgcg gtgggcgtgt cggagcgcct caagccgttc atgccgattc ccattgtcgg
      901 ccgcgaaggc gaccagttcc gctggttcga cgagcgcgac gtgccggaaa ccatcggccg
      961 cctgtccgcg ttcatgggca acgctggcgt gctgctgcgc gcgtacgtgt acgcgcgcat
     1021 gctggggcgc gagggcatgc accgcgtggc cgagttcgcc gcgctcaatg ccaactacct
     1081 ggccgccgcg ctcaaggcga agggttttga cctcgcgttc gatggccgcc gcgcttcgca
     1141 cgaattcatc atcacgctga agaagcagaa gcaggagatc gaactgaccg ccaccgacgt
     1201 ggccaagcgc ctgctcgact acggcttcca cgcgccaacc atctacttcc cgctgctggt
     1261 gccggaatgc ctgctgatcg agccaaccga gaccgaggac aaggaaacgc tcgacgcgtt
     1321 cgtcggtgcc ctggtgtcga tctgggacga ggcgaagaaa gacgtgcagt acgtcaaggg
     1381 cgcgccgtac accatgccgg tgcgccggct cgatgacgtt cgcgcagcgc ggcaactgga
     1441 cctgcgctac caggcatcca ggtagacgtc atggcagacg gcctgaaagg ctggaaacgt
     1501 ttgtggcgcg cgaccgtggt ctcgtactgg ggcctcggct atgcgctgcg caacgaggaa
     1561 gccttccgca tcgagttcgc gctgtgcgtg ctgctggcgc cgttggcact gtggctgggc
     1621 cacaacaagg tcgagcgcat catcatgctg atgtcgctgt tcgtcgtgct gatcacggaa
     1681 ctgctcaatt cgggcatcga agtggccatc gaccgcatca gcaaggagca gcacccgctg
     1741 tcgggcgcgg ccaaggatct gggctcggcg gcggtgcacc tgtccctgct gcaggttccg
     1801 ctggtgtgga gcctggtgtt gttcacctga aaaacctttc ggaaaaaaag aaatgtcggc
     1861 cctgatctgc ggttccttcg ccttcgacac catcatggtg tttcccggcc agttcaagaa
     1921 tgaaatcctg ccggacaagg tgcacatgct gaacgtgtcg ttcctggtgc cggaactgcg
     1981 ccgcgagttc ggcggcaccg ccggcaacat cgcctacaac ctcaacctgc tcggcggctc
     2041 ggtattgccg atgggtacgg tgggcgacga cttcgacaat tacgccgcgt ggatggacca
     2101 gtgcggcatc tcgcgcaagt acgtgaaaca ggtggccggc acctacaccg cgcaggctta
     2161 catcaccacc gacctcgacg acaatcagat caccgcgttc catccgggcg cgatgaacca
     2221 cgcgcacacc caggagatcc cggccaaggg catcaagatc ggctcgatct cgcccgacgg
     2281 ccgcgacggc atgatccagc acgcggcgca gtttgccgac gccggcgtgc cgttcctgtt
     2341 cgaccccggc cagggcctgc cgatgttcac cggcgccgag ctgcgcagct tcatccagca
     2401 ggccagttac tgcgcggtga acgactacga gtcggagctg ctgatggagc gcaccggctg
     2461 gaagctcaag cagatcgcgg agcggctcga cgcgctgatc gtcacccgcg gcggcaaggg
     2521 ctcgcacatc atcaccaaga agaagacctt cgagatcccg gtggcgcagg ccagcgcgct
     2581 ggccgatccc accggctgcg gcgacgccta tcgcggcggc ctgctctacg gattgcagaa
     2641 aggcatggac tgggaaacca ccggccgcat tgcggcgctg atgggcgcca tcaacatcga
     2701 gcggccgggc acgcagaacc acaagttcac gctcgaacag ttccgcgcgc gcttcaagaa
     2761 agaattccgc tacgagctgc gctgaaagtg ctcacgctca agctcaaatc ccacgaggag
     2821 cgccggctgc gcgccggcca cctgtgggcg tattcgaacg agatcgagac cgacgccggc
     2881 tggaagaaca ttgcacccgg cacgctgtgc cggctcaacg actcgcgcgg gcagccgctc
     2941 ggcatcggtt acgtgaacg
//
LOCUS       DASZZC010000203         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1042135,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000203 DASZZC010000000
VERSION     DASZZC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042135"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VHE37_04420"
     CDS             <1..988
                     /locus_tag="VHE37_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT34798.1"
                     /translation="GSDGNAWIGGLPFPDAKTGEEAIANLTLSWGRHDYCQYAIRQWD
                     INPAGDEAYQYDFVWAELQCNARLDGKPYADKKDLLRLQSVWFTGPQDVAGSSFLSIW
                     YYDQRKFPDLYGYFPAFKRVRQFPTNQRFEPLLPGITWFLTDAWAAGDPMLTWGNYKI
                     IGRQPMLGPQTENWNGSKKNWEYGVHGGPKNKTFLDTNFSMCPECIVLEAEPTGYPRA
                     PVGKKRVWIDVRNQQFVAYITYDRRGEIWKSFEPGWGLYKDGSAVVKDTKGRPEWSWT
                     YVMSHDIQANRMSRVMHTEACTGGYKSVFATNGVDVYNKYLTVNAISRLGAA"
     gene            1183..2133
                     /locus_tag="VHE37_04425"
     CDS             1183..2133
                     /locus_tag="VHE37_04425"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HVT34799.1"
                     /translation="MKHPLTALFAATFALVAQAAAAPTEALVTGVAHDALFTINFDGQ
                     NGIAGGAPGRVLATHDGGKSWTSDKTFPSPLAVLGADVKGERTLAVGQMGTIFLREGS
                     GAWKKIDSGSTERLMNVSLNSHGRAVAVGSFGTVLKSDDGGASWASIAPEWKPYLTAD
                     QSDQGIQPHMSAVQVGEDGVITIAGEFSLILRSEDGGKSWKQAYKNEPSIFALQLRGD
                     GVGYAVGQDGFIARSADGGKSWMPLTGAGKAILLGVGSDGSHVVIAGMHDMLASDDDG
                     KSFHHVQSEDVQSAWYAGVAVAGGVFYGVGHNGRIVKINS"
     gene            2313..>3326
                     /locus_tag="VHE37_04430"
     CDS             2313..>3326
                     /locus_tag="VHE37_04430"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT34800.1"
                     /translation="MTKQLATLFLLTLMLGATAHAQDAAAPAPADAAPPADTAAAPAD
                     AAPAPAADPAATNSEAPAPAPEIPVAGDAPAPVEQPGMFSDIDVSFSGFLRTETTVSA
                     TKEDNPANQLGNVFNGSAQPRRAYTPPGYLPATCAGVTARAALGALGSALCPAAGLAV
                     PSWTTLAFPGALAGDASTPGLRGTFSANADGSLTGTPFPLVDNVMNLQLIRGEGEMNV
                     KFGPDLAFIAHLRAVYDPAVYKEFDAHSVDHTITAGGAASGGLNSGGINGGDPHLYAG
                     KPDYFDYITEGGRKGHRLEWTGRNYQIYLPTLVLDYNHGPLNVRIGNQQIAWGQALFF
                     RVFD"
BASE COUNT          595 a         1136 c         1096 g          499 t
ORIGIN      
        1 cggcagcgac ggcaacgcct ggatcggcgg cctgccgttc cccgatgcca agaccggcga
       61 agaagccatc gccaatctca cgctgagctg gggtcgccac gattactgcc agtacgccat
      121 ccgccagtgg gacatcaacc cggccggcga cgaggcgtat caatacgact tcgtctgggc
      181 cgagctgcag tgcaacgcgc gcctcgacgg caaaccctac gccgacaaga aggacctgct
      241 gcgcctgcag tcggtgtggt tcaccggacc gcaggacgtc gccggttcct cgttcctgtc
      301 gatctggtac tacgaccagc gcaagttccc ggacctctac ggctacttcc ccgcgttcaa
      361 gcgcgtgcgc cagttcccga ccaaccagcg cttcgagccg ctcttgcccg gcatcacctg
      421 gttcctcacc gacgcctggg ccgcaggcga cccgatgctg acctggggca actacaagat
      481 catcggccgc cagccgatgc tgggcccgca gaccgagaac tggaacggca gcaagaagaa
      541 ctgggaatac ggcgtgcacg gcgggccgaa gaacaagacc ttcctcgaca ccaatttctc
      601 gatgtgcccg gaatgcatcg tgctggaggc cgaacccacc ggctatccgc gcgcgccggt
      661 cggcaagaag cgcgtgtgga tcgacgtgcg caaccagcag ttcgtcgcgt acatcaccta
      721 cgaccgccgc ggcgagatct ggaaatcgtt cgagccgggc tggggtctgt acaaggacgg
      781 cagcgcggtg gtgaaggaca ccaagggccg cccggagtgg tcgtggacct acgtgatgag
      841 ccacgacatc caggccaacc gcatgtcgcg cgtgatgcac accgaagcct gcaccggcgg
      901 ctacaagtcg gtgttcgcca ccaacggcgt ggacgtctac aacaagtacc tcacggtcaa
      961 cgccatctcg cggctgggtg cagcgtaagt gaagccaacg aagattcgtg cggttcgcgt
     1021 cccttccccc tgcgggggaa ggtggcgcgc agcgccggat gagggtcgct gcccaaccgg
     1081 gcgttgcacc ttgctgaaag ttggtccctc acccgcgcct gcggcacgac ctctcccggc
     1141 gggagaggct agccagtgca atttctgctg agggtctcat cgatgaaaca tccgctgacg
     1201 gccctgtttg ctgcgacctt cgcgctggtc gcccaggccg cggccgcacc gaccgaggcg
     1261 ctggtcaccg gcgtggcgca cgatgcgctg ttcaccatca acttcgacgg ccagaacggc
     1321 attgccggcg gcgcacccgg ccgcgtgctg gccacgcacg acggcggcaa gagctggacc
     1381 tcggacaaga ctttcccgtc gccgctggcg gtgctcggcg ccgacgtgaa aggcgagcgc
     1441 accctcgcgg tcggccagat gggcaccatc ttcctgcgcg aaggcagcgg cgcgtggaag
     1501 aagatcgaca gcggcagcac cgagcggctg atgaacgtga gcctcaacag ccatggccgc
     1561 gcggtggcgg tgggttcttt cggcaccgtg ctcaagtcgg acgacggtgg cgccagctgg
     1621 gcgtcgatcg cgccggagtg gaagccgtac ctcaccgccg accagtccga ccagggcatc
     1681 cagccgcaca tgtcggcggt gcaggtgggc gaggacggcg tcatcaccat cgccggcgag
     1741 ttcagcctga tcctgcgcag cgaagacggc ggcaagagct ggaagcaggc gtacaagaac
     1801 gagccttcga tcttcgcgct gcagctgcgc ggcgacggcg tcggctacgc ggtgggccag
     1861 gacggcttca ttgcgcgcag cgccgacggc ggcaagtcgt ggatgccgct gaccggcgcg
     1921 ggcaaggcga tcctgctcgg cgtcggctcg gacggcagcc acgtcgtcat cgccggcatg
     1981 cacgacatgc tggccagcga cgacgacgga aaaagttttc atcacgtgca gagcgaggac
     2041 gtgcagagcg cgtggtacgc cggcgtggcg gtggccggcg gcgtgttcta cggcgtgggc
     2101 cataacggcc gcatcgtcaa gatcaacagc tgatggagtg cggagacgcc gaaaaagccg
     2161 gcaccgcacc ccgccagtgt gtgttattaa ttattgtttg atttttaatt tgtaagatat
     2221 tttataacac cgacgacagc ggtgcggtgg acggaaaaag ccagcgccga ctggtgcaaa
     2281 ggagctgcgg tgcgactgct tgggagtaag caatgacgaa acagcttgcg acgctattcc
     2341 tcctcacgct gatgctgggc gccacggccc acgcgcagga cgccgccgcg ccggcgcctg
     2401 ccgatgccgc gccgccggct gacaccgccg cagcgccggc tgatgcggcc cccgctcccg
     2461 ccgccgaccc cgctgctacg aattccgaag cacccgcacc cgcgccggaa attccggtgg
     2521 cgggcgacgc gccggcgccg gtggaacagc cgggcatgtt ctccgacatc gacgtctcct
     2581 tctccggctt cctgcgcacc gagaccacgg tgtcggcgac caaggaggac aacccggcca
     2641 accagctggg caacgtgttc aacggctcgg cgcagccgcg ccgggcctac acgccgccgg
     2701 gctacctgcc ggccacctgc gctggcgtca ccgcgcgcgc cgcgctgggc gcgctgggca
     2761 gtgcgctgtg cccggccgcc gggctggccg tgcccagctg gaccaccctg gcgtttcccg
     2821 gcgcgcttgc cggcgacgcc tccacgcccg ggctgcgcgg cactttctcc gccaatgccg
     2881 acggctcgct caccggcacg ccgttcccgc tggtcgacaa cgtgatgaac ctgcagctga
     2941 tccgcggcga gggtgagatg aacgtgaagt ttgggcccga cctcgccttc atcgcgcacc
     3001 tgcgcgcggt gtacgacccg gcggtgtaca aggaattcga cgcgcacagc gtggaccaca
     3061 ccatcaccgc cggcggcgcg gccagcggcg gactcaacag cggcggcatc aacggcggcg
     3121 atccgcacct gtatgcgggc aagccggatt acttcgacta catcaccgag ggcgggcgca
     3181 aaggccaccg gctggaatgg accgggcgca attaccagat ctacctgccc acgctggtgc
     3241 tcgattacaa ccacggcccg ctcaacgtgc gcatcggcaa ccagcagatc gcctggggcc
     3301 aggcgctgtt cttccgcgtg ttcgac
//
LOCUS       DASZZC010000204         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1236235,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000204 DASZZC010000000
VERSION     DASZZC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236235"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VHE37_04435"
     CDS             complement(<1..464)
                     /locus_tag="VHE37_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34801.1"
                     /translation="MIRASFIEYEGAAPQPVTLHIMSGMVWIKTGDVIERRVALSDLK
                     IAPLRGTSQKLTLPDGSWCDIPDQPAITELIEEVKGDTVRLAPAEREPVLQRLAGALF
                     TFVLLLVAAAVAVWSWGVPHAAQTLAAQMPAAQRAALGQRVLASLDADGTLTP"
     gene            complement(461..1489)
                     /locus_tag="VHE37_04440"
     CDS             complement(461..1489)
                     /locus_tag="VHE37_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVT34802.1"
                     /translation="MSIAADYYDGRSSRPQPVTLHGAGGRLFLTTADGSERACDGFEI
                     TERLGTTPRRLNFADGSFCEAAHGEPLQQLLRELGRGAPAIEHAQHSWRVALLAVVAT
                     VMIGVAAYLYGLPAAADVISAHLPADVVDALSEQTLEVLREPELSVPSKLPVQRQRAL
                     RAGFDALKTPDGQPQVAHEFLFRSSPLIGANAMALPDGHILLLDELVQLADNDPQIYA
                     VLAHELGHVAHRHSLRLLVQGSAVGALSAWWFGDASSLLIAGPTVLLQTRYSRAFESE
                     ADAYAAAMMRANGLSPMLLAQMLEKLVRSHHAESAHDGWLDYVSSHPAAAERIRLLGG
                     TPPAEAAP"
     gene            complement(1486..2565)
                     /locus_tag="VHE37_04445"
     CDS             complement(1486..2565)
                     /locus_tag="VHE37_04445"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HVT34803.1"
                     /translation="MESESIMPAVAPAEPLPPQRLRFTGDGGEYFQIWIVNLLLTVLT
                     LGIYSAWAKVRRLRYFYRNTELAGARFEYHGDPIAILKGRMIALGLLLAYQFAPRVSM
                     RLFALVLLLLAVVMPWLLRQSFRFRMHNSSWRGLRFSFHGSVAEAYTSFLVLPIFSLI
                     TLYLGAPLMHQRIKQYQHGNARYGSERFGFHATAGNFYGAYWIIMLLFLLLIIAGGVV
                     SGMAIAAASAGHGGKPNASAVLGGMLWMFGVIVAGFLAIGPLWLARIQNLVWNNTTLG
                     AHRFESKVHAGRLIWITLTNFIGIVVTLGLFRPWADVRVARYLLESVALLPASSLDEF
                     VAGQEQAMGAVGQEAADLFDIDIGL"
     gene            complement(2584..>4042)
                     /gene="mutL"
                     /locus_tag="VHE37_04450"
     CDS             complement(2584..>4042)
                     /gene="mutL"
                     /locus_tag="VHE37_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HVT34804.1"
                     /translation="SILSVSRLTLTSRAGGAEHGWQTGGEGQLQMLPPRPAAHPPGTT
                     VEVRDLFFNTPARRKFMKADATEFRHVQQLLSRIALARFSAGFALTHNGRKVFDLRAA
                     DDDGARANRVGEICGAEFLDNAIALEREQGGLRLQGWVGVPAFARSAPDLQFLYVNGR
                     AVRDRLLGHALRQAYADVMHSQHYPAFVLYLEMDAAGVDVNVHPQKTEVRFREGNRVH
                     DFVTSAVQAALRALRPQPALHQLRIAPPEMPSPQQPMRYSIPPAAPRPLAESAPARLE
                     QTWAALRAPPQEQPDELPLGHAVAQLHGIYILAQNRHGLVLVDAHAAHERVLYERCKR
                     DLSRGAIATQALLLPVMVPLHEDQAESLEARREELARVGVQIDRAGPATIAVRAVPPL
                     LAREDIAGFVRRLVGEEDGPAHFGEVLDAQHRVMADMACKAAIKAHRELRLPEMDALL
                     RDMEQTELSGQCNHGRPTWMQLSMDELDRLFLRGR"
BASE COUNT          657 a         1416 c         1371 g          598 t
ORIGIN      
        1 ggggtgagcg tgccgtcggc gtccagcgag gcgagcacgc gctggcccag cgccgcgcgc
       61 tgcgccgcgg gcatctgcgc cgccagggtc tgcgccgcat gcggcacacc ccaggaccac
      121 accgccaccg ctgcggccac cagcagcagc acgaaggtga acagcgcgcc ggccagccgc
      181 tgcagtaccg gttcacgttc cgccggcgcc agccgcacgg tatcgccctt cacttcctcg
      241 atcagttcgg tgatcgccgg ctggtcgggg atgtcgcacc aggagccatc gggcaacgtg
      301 agcttctgcg aggtgccgcg cagcggtgcg atcttcaggt cagacaaggc cacgcgccgc
      361 tcgatgacat cgccggtctt gatccacacc atgcccgaca tgatgtgcag cgttaccggc
      421 tgcggcgcgg caccttcgta ctcgatgaag ctggcgcgga tcacggcgct gcctccgccg
      481 gcggcgtgcc gccgagcagg cggatgcgct cggccgctgc cggatgcgac gagacgtagt
      541 cgagccagcc atcgtgcgcc gattcggcgt gatgcgagcg caccagcttc tccagcatct
      601 gcgccagcag catcgggctg aggccattgg cgcgcatcat cgcggcggca taggcgtcgg
      661 cctcgctctc gaacgcgcgc gagtagcgcg tctgcagcag caccgtcggc cccgcgatca
      721 gcagcgaact ggcatcgccg aaccaccagg cgctgagcgc tcccaccgcg ctgccctgca
      781 ccagcaggcg cagcgagtgc cggtgcgcca cgtggccgag ttcgtgcgcc agcacggcat
      841 agatctgcgg gtcgttgtcg gccagctgca ccagctcgtc cagcagcagg atgtggccgt
      901 cgggcagcgc catggcgttg gcgccgatca gcggcgagga gcggaacagg aattcgtgcg
      961 ccacctgcgg ctggccgtcc ggtgtcttca gcgcatcgaa gccggcgcgc aatgcacgct
     1021 ggcgttgcac cggcagcttg ctcggcacgc tcagctccgg ctcgcgcagc acctcgagcg
     1081 tctgctccga caacgcgtcc accacatcgg cgggcaggtg ggcggaaatg acgtccgctg
     1141 cagccggcag cccgtacagg taggcggcca cgccgatcat caccgtcgcc accaccgcca
     1201 gcagcgccac acgccagctg tgctgtgcat gctcgatcgc cggcgcaccg cgcccgagct
     1261 cgcgcagcag ctgctgcagc ggctcgccgt gggccgcctc gcagaaactg ccgtcggcga
     1321 aattcagccg ccgtggcgtg gtgcccagcc gttcggtgat ctcgaagcca tcgcacgcac
     1381 gctcactgcc gtctgccgtg gtgaggaaca gccggccacc cgcgccgtgc agcgtcaccg
     1441 gctgcggccg tgagctgcgc ccgtcgtagt agtcggctgc gatgctcaca ggccgatgtc
     1501 gatgtcgaac aggtcggccg cttcctggcc gaccgcgccc atcgcctgct cctggccggc
     1561 gacgaattca tccaggctcg acgccggcag cagcgctacc gattccagca ggtagcgcgc
     1621 cacgcgcaca tcggcccacg gccggaacag gcccagggtg accacgatgc cgatgaaatt
     1681 ggtcagcgtg atccagatca gccgcccggc gtgcaccttg ctctcgaagc ggtgcgcacc
     1741 cagcgtggtg ttgttccata ccaggttctg gatgcgcgcc agccacagcg ggccgatggc
     1801 caggaagccg gcgacgatca cgccgaacat ccacagcatg ccaccgagaa cggcggaggc
     1861 attgggcttg ccgccatggc cggcgctcgc tgccgcgatg gccatgcccg ataccacccc
     1921 gccggcgatg atcagcagca ggaacagcag catgatgatc cagtaggcgc cgtagaaatt
     1981 gccggcggtg gcatggaagc cgaaacgctc gctgccatag cgcgcattgc cgtgttggta
     2041 ctgcttgatg cgctggtgca tcagcggcgc gccgagatag agcgtgatca gcgagaagat
     2101 cggcagcacc aggaagctgg tgtaggcctc ggccacgctg ccgtgaaacg agaagcgcag
     2161 gccgcgccag ctgctgttgt gcatgcggaa gcggaacgat tgccgcagta gccacggcat
     2221 caccacggcc agcagcagca gcaccagcgc gaacagccgc atcgagacgc gtggcgcgaa
     2281 ttggtacgcc agcagcagtc cgagcgcgat catgcggccc ttgaggatgg cgatgggatc
     2341 gccgtggtac tcaaagcgtg caccggccag ttcggtgttg cggtagaagt agcgcagccg
     2401 ccgtaccttg gcccaggccg agtagatgcc cagcgtcagc acggtcaaca gcaggttgac
     2461 gatccagatc tggaaatact cgccgccatc gccggtgaaa cgcagccgct gcggcggcag
     2521 tggctctgcg ggcgctaccg ccggcatgat cgattcgctt tccatctttc cactccccgt
     2581 tgatcaacgt cccctgagaa acagccggtc gagctcgtcc atggacagct gcatccaggt
     2641 cggccggccg tggttgcact gaccggacaa ttcggtctgc tccatgtcgc gcagcagggc
     2701 gtccatctcc ggcaggcgca gttcgcggtg cgccttgatc gccgccttgc aggccatgtc
     2761 ggccatcacg cggtgctgcg cgtcgagcac ctcgccgaaa tgcgcagggc cgtcctcctc
     2821 gcccaccagc cgccggacga agccggcgat gtcctcgcgc gccagcagcg gcggcaccgc
     2881 gcgtacggca atggtggccg ggccggcacg gtcgatctgc acacccaccc gcgccagttc
     2941 ctcgcggcgg gcttccagtg actcggcctg gtcctcgtgc aagggcacca tcaccggcag
     3001 cagcagcgcc tgcgtagcga tcgcaccgcg ggacaggtca cgcttgcagc gctcgtacag
     3061 cacgcgctcg tgcgccgcgt gcgcgtcgac cagcaccagc ccatggcggt tttgcgccag
     3121 gatgtagatg ccgtgcagtt gcgccaccgc gtggcccagc ggcaactcgt ccggctgttc
     3181 ctgcggtggc gcgcgcagcg cggcccaggt ctgttccagg cgtgccggtg cgctttcggc
     3241 cagcgggcgc ggcgcggccg gcggaattga atagcgcatc ggctgctgcg gcgacggcat
     3301 ttccggcggc gcaatgcgca gctggtgcag cgccggctgc gggcgcaggg cgcgcaatgc
     3361 cgcctgcacc gcgctggtga cgaagtcgtg cacgcggttg ccctcgcgga aacgcacctc
     3421 ggtcttctgc gggtgcacgt tgacgtcgac accggccgca tccatctcga ggtacagcac
     3481 gaaggcagga tagtgctggc tgtgcatcac gtcggcataa gcctggcgca gtgcgtggcc
     3541 gagcaggcgg tcgcgcacgg cgcggccatt cacgtacagg aactgcaggt cgggcgcgga
     3601 gcgcgcaaac gccggcacgc cgacccagcc ctgcaggcgc agtccaccct gctcgcgttc
     3661 cagcgcgatg gcgttgtcca ggaattcggc gccgcagatc tcgccgacgc ggttcgcacg
     3721 cgcgccgtca tcatcggccg cgcgcagatc gaataccttg cgcccgttgt gcgtcagcgc
     3781 gaaccccgca ctgaaacgtg ccagggcgat gcgcgaaagc agctgctgca cgtggcggaa
     3841 ctcggtggcg tcggccttca tgaacttgcg ccgcgccggc gtgttgaaga acaggtcgcg
     3901 cacttccacc gtggtgccgg gcgggtgcgc cgcgggacgc ggcggcagca tctgcaactg
     3961 gccctcgccg ccggtctgcc agccgtgttc ggcgccgccg gcacgcgaag tcagcgtcag
     4021 gcgcgagacc gaaagaatgc tg
//
LOCUS       DASZZC010000205         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_979662,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000205 DASZZC010000000
VERSION     DASZZC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979662"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1679
                     /locus_tag="VHE37_04455"
     CDS             123..1679
                     /locus_tag="VHE37_04455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HVT34805.1"
                     /translation="MTALLAIDVGTQSARAIVFDESGRLLAKAQVPIEPVYHSPQPGW
                     AEQDPEVYWNAVVLACQQLWAQKKIAPHAIAGLSLTTQRATMICADAAGKPLRPAILW
                     LDQRRCETPPDMGALWNTLFRVAGASGIVRHLQREADANWLAAHQPEVWKRTDKFLFL
                     SGWLTHRLVGAFVDSSGCQVGYVPFEYKKLTWAAPSDFKWKAISATRAQLPDLVPPGA
                     KLGALAAAAASVLGLPAGLPVIAAAADKACEVLGCGVLEPDVGQLSFGTTATINTTQP
                     KYMEVQRLLPAYPAAMPDAWNTEIQIFRGFWMVSWFKREFGLREETLARERGVAAEEL
                     FDDLIKQVPPGSMGLTLQPYWSPGVRDPGPEAKGAIIGFGDVHTRAHVYRAILEGLVY
                     GLRAGREQIERRTGKPIRLLRIAGGGSQSDTAMQIAADVFGLPAERPEVYETSALGAA
                     INLAVGLGVHRDYAGAVAVMTRPGRRFEPDAATQKIYDRLYREVYRELYPSLKPLYQR
                     IRDITGYPPP"
BASE COUNT          275 a          591 c          575 g          263 t
ORIGIN      
        1 caggtattgc cgcgcgtgct cgaactctgc cgcgcggaac tgggctggga cgaaacgcgc
       61 tgcgcgcaag aagaacagcg ctaccgtgaa ctctggcagc agaaacacgc gccggcgctc
      121 gcatgacagc gctgctcgcc atcgatgtcg gcacgcagag cgcgcgcgcc atcgttttcg
      181 atgagtccgg ccgcctgctg gcaaaggcgc aggtgccgat cgaacccgtc tatcactcgc
      241 cgcaacccgg ctgggccgag caggacccgg aggtttactg gaatgccgtg gtgctggcct
      301 gccagcaatt gtgggcgcag aaaaaaatcg cgccgcatgc gatcgccggc ctttcgctga
      361 ccacgcagcg cgcgaccatg atctgcgccg acgcggccgg caagccgctg cgcccggcca
      421 tcctctggct cgaccagcgc cgctgcgaga caccgcctga catgggcgcc ctgtggaaca
      481 cgctgttccg cgtggcgggg gcgagcggca tcgtcaggca tctgcagcgc gaagccgacg
      541 ccaactggct cgccgcgcat caacccgagg tgtggaagcg cactgacaag ttcctgttcc
      601 tgtcgggttg gctcacgcat cgcctggtcg gcgccttcgt ggattcgtcg ggttgccagg
      661 taggctacgt tccgttcgag tacaagaaac tcacctgggc ggcgccatcg gatttcaagt
      721 ggaaagcgat cagcgcgacg cgcgcgcaac tgccggacct ggtgccgccc ggcgccaagc
      781 tcggcgcgct cgctgctgcc gctgcttcgg tgctcggctt gcccgcgggt ctgccggtga
      841 tcgcggcggc ggccgacaag gcctgcgaag tgctcggctg cggagtgctc gaacccgatg
      901 tcggccagct ctctttcggc accaccgcca ccatcaacac cacgcagccg aaatacatgg
      961 aagtgcagcg cctgctgccg gcgtaccccg cggccatgcc cgacgcgtgg aacaccgaga
     1021 tccagatctt ccgcggcttc tggatggtga gctggttcaa gcgcgagttc ggcctgcgcg
     1081 aggaaacgct ggcgcgcgag cgcggcgtcg ccgccgaaga actgttcgat gacctgatca
     1141 agcaagtgcc gcccggctcg atgggcctga cgctgcagcc gtactggtcg cccggcgtgc
     1201 gcgatcccgg gccggaagcc aagggcgcca tcatcggctt cggcgacgtg cacacgcgcg
     1261 cacatgtgta ccgcgcgatc ctcgagggcc tggtgtacgg tctgcgcgcc gggcgcgagc
     1321 agatcgagcg gcgcaccggc aagccgatcc gcctgctgcg catcgccggc ggcggctcgc
     1381 agagcgacac cgcgatgcag atcgccgccg acgtgttcgg actgccggcg gagcggcccg
     1441 aggtgtacga gacttccgcc ctcggcgcgg ccatcaatct ggcggtcggg ctgggcgtgc
     1501 atcgcgacta cgccggcgcg gtcgccgtga tgacgcggcc gggccggcgc ttcgagcctg
     1561 acgccgccac gcagaaaatc tacgaccgcc tgtatcgcga ggtttaccgc gagctgtatc
     1621 ccagcctgaa gccgctgtac cagcgcatcc gcgatatcac gggctatccg ccgccgtagt
     1681 cgccgcggct atttgccgcg cacc
//
LOCUS       DASZZC010000206         7179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_530204,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000206 DASZZC010000000
VERSION     DASZZC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530204"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VHE37_04460"
     CDS             <1..575
                     /locus_tag="VHE37_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT34806.1"
                     /translation="QVNTSGILFICGGAFAGLEKIIQNRSATAGIGFSAEVKSKNDSK
                     QTSELLKIVEPDDLIRFGLIPEFVGRLPVLAVLEELDEKALMSILKEPKNALVKQYAK
                     LFHMEGCTLEIREDALKAIALKAKERKTGARGLRTILENILLDVMYDLPSTQNVSKVV
                     VDEAVVKGEVKPFLVYENADAKPALKSTAA"
     gene            complement(638..1060)
                     /locus_tag="VHE37_04465"
     CDS             complement(638..1060)
                     /locus_tag="VHE37_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HVT34807.1"
                     /translation="MSIDRPIHSFHRLRRIPSQGWVAGVLAGLAEYFDWNVKVLRVVC
                     AVALFCTGFFPIGFVYCVLWYVLDRDDAPSSRRGSSDDPPPRDYDPSPRYYRSRREPD
                     VTDVKVRFARLEERLRGMEECVASKDFELRRELRKLES"
     gene            complement(1050..1283)
                     /gene="pspB"
                     /locus_tag="VHE37_04470"
     CDS             complement(1050..1283)
                     /gene="pspB"
                     /locus_tag="VHE37_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HVT34808.1"
                     /translation="MVALVGEIAPFLFVITIVWLVLHYRHQRRTMRAGEAADSASHAE
                     LAVLAERMERRIEALEQILDAEAPGWRKRHHEH"
     gene            complement(1324..2034)
                     /gene="pspA"
                     /locus_tag="VHE37_04475"
     CDS             complement(1324..2034)
                     /gene="pspA"
                     /locus_tag="VHE37_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HVT34809.1"
                     /translation="MSIFSRLSDVINSNIHAMLDKAEDPEKMVRLIIQEMEDTLVEVR
                     STSVRTIARRKEVERNIAHLQAEAADWQRKAELAVSKDRDDLARGALAAKQSAQDHAA
                     ALQKELAHVDQEIDKLDDDTAKLKAKLADARQRQKTIVLRQASVASRLRVKGQLNDRK
                     MADTMLKMEQYEGKIDRLEAEVEAYDLGGRSLSDQIAALETNDKLEDELSQLRARLGK
                     PAPAAPVASAQGSTPSKE"
     gene            complement(2063..2329)
                     /locus_tag="VHE37_04480"
     CDS             complement(2063..2329)
                     /locus_tag="VHE37_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34810.1"
                     /translation="MKIQVFAAVMLASALAAPAQAADRDFSERVFAVMADTIAEQGNH
                     ALRDIRNEVRRNLVNTLKPLLPEPSNDADAGAETDTAAAGSAVQ"
     gene            2503..3555
                     /gene="pspF"
                     /locus_tag="VHE37_04485"
     CDS             2503..3555
                     /gene="pspF"
                     /locus_tag="VHE37_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HVT34811.1"
                     /translation="MATRAAPDPIIGQADNFLMALERVSRVAPLSKPVLIIGERGSGK
                     ELIASRLHYLSERWQRPYVKFNCAALNEELLESELFGHVAGAYTGATRPRVGRFELAH
                     GGTLFLDELASMSLRLQEKLLRVVEYGEYERVGSSETQSCDVRLVGAANVDLPALAAA
                     GKFREDLLDRLSFDVVTVPPLRERRDDILLLAQKFAEGMARELGREYFAGFAPAAERQ
                     LIAYAWPGNVRELRNVVERAVYRLDSAKRVVEQVEFDPFASPYRLPAQAAPATAAAPA
                     PVSAPASDDKVSFPLQFEPAVEAFETRILRAALDEARYNQRRAAELLGLTYHQLRGLL
                     RKYRLLGDETREPAGN"
     gene            3561..4655
                     /locus_tag="VHE37_04490"
     CDS             3561..4655
                     /locus_tag="VHE37_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HVT34812.1"
                     /translation="MHTVLVLGGYGFFGTRISTSLASAPGLHLLIGGRSFDRAQALAR
                     DLRLDPSAGVAIDADDQQLAHRFLALGVQTLIHTAGPFQGQDYRVARAAIEAGCHYID
                     LADSRAFVCGIGALDAPARERGVCVISGASSVPALSSAVVEQLRPQFSRMDSIRIGIS
                     TGARVPGLATVRAIFSYCGKPIRRLEQGRWLDTYGWLDMQRHRFPSPLGMRLLGSCDV
                     PDLDLLPRRYPSLQTTTFHAGISGAGHFAIWLGALVVRAGLLRSLASLAPLLHRAGVW
                     MEPAGSEHSGMYVELAGLDHAGHPLLRRWHLVAARHHGPYVPCGASVALARKLLSAES
                     LPPGAYPCTSLLTVEEYLEPLRDLDLRVLA"
     gene            complement(4677..6107)
                     /locus_tag="VHE37_04495"
     CDS             complement(4677..6107)
                     /locus_tag="VHE37_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HVT34813.1"
                     /translation="MIRAPFPCNAAVHRTCHAAIALLLCAALPAHANTPVAQLPALSI
                     AAPALPFADYRKPGPLRYAVAVPAAAGLTQGLWESDAGGHAVWRLRVHAPEASSISLT
                     LRGFHMPAGGALYFSALDGSHRRGPYGAEHNGAGQLWTALVPGSDALIEADVPAGMKD
                     ELALQIAQVHYGFLPFWKAGATDKSGSCNIDVVCPQGDAWRDEIRSVARYTIAGQYLC
                     SGQLLNDTGGDFTPHFLTANHCLSSEADAESTVFYWNYQTSTCGGTPDGNLDQNQSGA
                     TLEASYAPSDFTLLKLDAAPSGSFNVYYAGWDAANAAPSDGVVTIHHPGGDEKRISMS
                     SGPTDVESYGGGGDDGTHLRVQRWDEGTTEGGSSGAGLWNSAHHVVGQLHGGAASCDN
                     TSGSDWFGRFAVSWTGGGSATDSLAPNLDAGKSSLTVMDGADPNGKSTPLSSAAPSNA
                     SAARYGGALGFTTLLPLWWMRRRRRS"
     gene            6430..>7179
                     /locus_tag="VHE37_04500"
     CDS             6430..>7179
                     /locus_tag="VHE37_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT34814.1"
                     /translation="MWIVQIALRRPYTFIVLALLIVLLGGLAIFNTPTDIFPNIRIPV
                     VATIWRYTGLPPEDMANRVVLFSERTAQTVVNDVEHTESQSLNGTAVVKYFFQPHANE
                     DLSYSQITGVAQTQLAFSPPGTTPPFILAYNASSVPILQLAFTSETLPENQIFDQANS
                     VVRTALATVNGASIPFPYGGRQRQVQVDLDPAALRAYGLTPQDVSTAIGQQNLILPQG
                     SEKIGDLEYFIKLNASPTKVEELNDLPIKTRN"
BASE COUNT         1216 a         2331 c         2358 g         1274 t
ORIGIN      
        1 tgcaggtgaa caccagcgga attctgttca tctgcggcgg cgcttttgcc gggctggaga
       61 agatcatcca gaaccgcagc gccacggccg gcattggttt ctcggccgaa gtgaaatcca
      121 agaacgattc caagcagacc agcgaactgc tcaagatcgt ggagccggat gacctgatcc
      181 gtttcggcct gattcccgag ttcgtcggcc gtctgccggt actggccgtg ctggaggaac
      241 tggacgagaa ggcgctgatg tcgatcctca aggaaccgaa gaacgcgctg gtcaagcagt
      301 acgccaagct gttccacatg gaaggctgca ccctggagat ccgcgaggac gcgctcaagg
      361 ccatcgcgct gaaggccaag gagcgcaaga ccggcgcgcg cggcctgcgc accatcctgg
      421 aaaacattct gctcgacgtc atgtacgacc tgccgtccac gcagaacgtg agcaaggtcg
      481 tggtcgacga ggcggtggtg aaaggcgagg tgaagccgtt cctggtctac gagaacgcgg
      541 acgccaagcc ggcgctcaag agtaccgctg cctgattctc aaggcggcaa caaaaaaggc
      601 ccgcgcgatg cgggcctttt tcttttcgcg atgatgctca ggattcgagc ttgcgcaatt
      661 cgcgccgcag ttcgaagtcc ttcgacgcca cgcattcctc catgccgcgc aggcgttctt
      721 ccaggcgggc gaagcgcact ttgacgtcgg tcacgtccgg ctcgcgccgg gaccggtaat
      781 aacgcgggct gggatcatag tcgcggggcg gcggatcgtc gctgctgccg cggcgcgatg
      841 aaggcgcgtc gtcgcggtcg agcacgtacc acagcacgca atagacgaag ccgatcggga
      901 agaagccggt gcaaaacagc gcgacggcgc agaccacgcg cagcaccttg acgttccagt
      961 cgaaatattc cgccaggccg gccagcacgc cggcgaccca gccctgcgag gggatgcggc
     1021 gcagacggtg gaaggaatgg atggggcgat caatgctcat ggtgtctctt tctccagccg
     1081 ggagcttcgg catcgaggat ctgctccagc gcttcgatgc gccgctccat gcgctccgcc
     1141 agcaccgcga gttcggcgtg cgaagcgctg tcggcggctt cgccggcgcg catcgtgcgc
     1201 cgctggtggc ggtagtgcag caccagccag acgatggtga tgacgaacag gaacggcgcg
     1261 atttctccga ccagtgcaac catgtcgttc acctgctact ccctgataac accaacagcc
     1321 gtatcactcc ttggacgggg tggaaccttg cgccgatgcg accggagcag ccggcgccgg
     1381 cttgccgagc ctggcgcgca gctgcgacag ctcgtcttcc agcttgtcgt tggtttccag
     1441 cgcggcgatc tggtccgaca gcgagcggcc gcccagatcg taggcttcga cctccgcctc
     1501 gagacgatct attttgcctt catattgctc catcttcagc atggtgtcgg ccatcttgcg
     1561 gtcgttgagc tggcccttga cccgcaggcg cgacgccacc gaagcctggc gcagcacgat
     1621 ggtcttctgc cgctggcgcg cgtcggccag cttggccttg agcttggcgg tgtcgtcgtc
     1681 gagcttgtcg atttcctggt cgacatgcgc cagttccttc tgcagcgcgg cggcatgatc
     1741 ctgcgccgac tgcttggcgg ccaatgcgcc gcgcgccaga tcgtcgcgat ccttgctcac
     1801 cgcgagctcg gccttgcgct gccagtcggc agcctcggcc tgcaggtgcg cgatgttgcg
     1861 ctccacttcc ttgcgccggg cgatggtgcg cacgctggtc gaacgaactt cgacgagggt
     1921 gtcctccatc tcctggatga tcaggcgcac catcttctcg ggatcttccg ccttgtcgag
     1981 catggcgtga atgttcgagt tgatgacgtc gctcagtctg gaaaagatgc tcatgtcgtc
     2041 ctccgggaaa tcggcattcg tttcactgca ccgcgctacc cgcggcggca gtatcggttt
     2101 ctgcgcccgc atcggcgtcg ttcgacggct ccggcagcag tggcttgagt gtgttcacca
     2161 gattgcggcg cacttcattg cggatatcgc gcagcgcatg gttgccctgt tcggcgatgg
     2221 tgtcggccat cacggcaaac acgcgttcgg aaaaatcgcg gtcggcggcc tgggccggtg
     2281 cagccagcgc agaggccagc atcacggcgg cgaacacttg aattttcatg gcaactccct
     2341 gtgggttggc gtttgtacag ggggttattg ccagcggcgt gccaacttgg aaatgcgttt
     2401 caaactattg aaaataaatg aatatatttt aaggtcgcca agggcgccat cgtcagctac
     2461 tctagccttc ggcaaaaaaa accataagtt ggcgaaaacc acatggccac ccgagcggcc
     2521 ccggatccca tcatcggcca ggcggataac ttcctcatgg cgctggagcg cgtctcgcgc
     2581 gtggcgccgc tgtccaagcc ggtcctcatc atcggcgagc gtgggtccgg caaggagctg
     2641 atcgcctccc gcctgcatta cctgtcggag cgctggcagc gcccttacgt gaaattcaat
     2701 tgcgccgcgc tcaacgagga gttgctggag tccgaactgt tcggccatgt cgccggcgcg
     2761 tatacgggcg ccacgcggcc gcgcgtagga cgtttcgagc tggcgcatgg cggcacgctg
     2821 ttcctcgacg agcttgcttc aatgtccctg cgcctgcagg agaagctgct gcgcgtggtg
     2881 gagtacggcg aatacgagcg cgtcggctcg agcgaaacgc agagctgcga cgtgcgcctg
     2941 gtcggcgcgg ccaacgtcga cctgccggcg ctggcggccg cgggcaagtt ccgcgaagac
     3001 ctgctcgatc gactctcgtt tgacgtggtg acggtgccgc cgctgcgcga acgccgtgac
     3061 gacatcctgc tgctggcgca gaaattcgcc gaaggcatgg cgcgcgaact gggccgcgaa
     3121 tacttcgccg ggtttgcgcc ggccgcggag cgccagctga tcgcttacgc atggccgggc
     3181 aacgtgcgcg agttgcgcaa cgtggtcgag cgcgcggtgt atcggctgga cagcgccaag
     3241 cgggtggtgg agcaggtcga gttcgatccg tttgcttctc cctatcgtct tcctgcgcag
     3301 gccgcgccgg caacagccgc agcacccgcg cccgtttccg cgccggcatc ggacgacaag
     3361 gtgagtttcc cgctgcagtt cgagccggcg gtggaagcct ttgagacgcg catcctgcgc
     3421 gcggcactcg atgaagcgcg ctacaaccag cggcgtgccg cggaattgct cgggctcacc
     3481 tatcaccagc tgcgcgggct gctgcgcaaa taccggctgc tcggcgacga gacgcgcgag
     3541 cctgccggca actgagccag atgcacacag tccttgtgct gggcgggtat ggatttttcg
     3601 gcacgcgcat cagcaccagt cttgcgtccg caccgggcct ccacctgctg atcggcggcc
     3661 gaagtttcga tcgcgcacaa gcgcttgcgc gggacttgcg gctcgatcca tcggcaggcg
     3721 tggcaatcga tgcggacgac cagcaacttg cacatcgctt ccttgcgctc ggtgtgcaga
     3781 ccctgatcca caccgcaggc ccgttccagg ggcaggatta ccgcgtggcg cgcgcggcca
     3841 tcgaggccgg ctgtcattac atcgatctcg ccgacagccg cgccttcgtt tgcggcatcg
     3901 gtgcactgga cgcgcccgcg cgcgaacgcg gcgtctgcgt gatcagcggg gcaagctcgg
     3961 tgcccgcgct gtcctcggcc gtggttgagc agctgcggcc gcagttttcc cggatggact
     4021 cgatccgcat cggcatcagc acgggggcgc gtgtgccggg cctggccacc gtgcgggcga
     4081 ttttcagcta ctgcggaaag ccgatccggc gcctggagca ggggcgctgg ctggacacgt
     4141 acggctggct cgacatgcag cgccaccgtt ttccatcacc cctgggcatg cgccttctag
     4201 gcagttgcga tgtgcccgat ctcgacctgc tgccacggcg ctacccgagc ctgcagacga
     4261 cgaccttcca cgctggcatc agcggtgccg gacattttgc aatttggctg ggcgccctcg
     4321 tcgtaagggc cggcctcctg cgcagtctcg cgtcgctggc gccgctgctc catcgcgccg
     4381 gcgtctggat ggaaccggcc ggaagcgagc acagcgggat gtatgtcgag cttgcggggc
     4441 tggatcatgc cggccatccg ttgttgcgcc gctggcatct ggtcgccgcg cgccaccacg
     4501 ggccctatgt gccctgcggc gcgtcggtgg cactcgcgcg gaaactgcta tcggcggaaa
     4561 gcctgccgcc cggcgcttat ccatgcacgt cgctgttgac ggtcgaagaa tacctggagc
     4621 cgctgcgcga cctcgatctg cgagtgctgg cgtagcccga tccatccgcc gcgggatcag
     4681 gatcgccggc gccggcgcat ccaccacagc ggcagcaggg tggtgaaacc cagcgcgccg
     4741 ccgtagcgcg cggcacttgc gttcgaggga gcagcgctgg acagcggcgt ggattttcca
     4801 ttggggtcgg cgccgtccat caccgtcagc gatgatttgc ccgcgtccag gttcggcgcg
     4861 aggctgtccg tcgccgagcc gccgccggtc cagctcaccg caaagcggcc gaaccagtcg
     4921 gaaccggagg tgttgtcaca cgaagccgcg ccgccatgca gctggccgac gacgtgatgc
     4981 gcgctgttcc acaggccggc gccggacgag ccgccctcgg tggtgccttc atcccatcgc
     5041 tgtacgcgca gatgcgtgcc gtcgtcgccg ccgccgccgt acgactccac gtcggtgggc
     5101 ccgctggaca tgctgatgcg cttctcgtca ccgcccgggt ggtggatggt gaccacgcca
     5161 tcggacggcg ccgcgttcgc cgcgtcccag ccggcgtaat acacattgaa tgagccgctg
     5221 ggcgccgcgt cgagcttgag cagggtgaag tcggaaggcg catagctcgc ttccagcgtc
     5281 gcgcccgact ggttctggtc gaggttgccg tcgggcgtgc cgccgcaggt gctggtctgg
     5341 tagttccagt agaacacggt cgactcggca tcggcctcgc tcgacaggca atgattggcg
     5401 gtgaggaaat gcggcgtgaa atcgccgccg gtatcgttca gcagttgtcc gctgcacagg
     5461 tactgcccgg cgatggtgta gcgcgccacc gagcggatct cgtcgcgcca ggcatcgcct
     5521 tgcgggcaga cgacatcgat gttgcagctg ccggacttgt ccgtcgcgcc ggctttccag
     5581 aatggcagga agccgtaatg cacctgcgca atctgcagcg ccagctcatc cttcatgccg
     5641 gccggcacat cggcttcgat cagcgcatcg ctgccgggca ccagcgcggt ccacagctgt
     5701 ccagccccgt tgtgctcggc gccgtaaggt ccgcgccgat ggctgccatc gagggccgag
     5761 aagtagagcg cgccgcccgc cggcatgtgg aatccgcgca gtgtgagcga gatcgacgag
     5821 gcctccggcg cgtgcacgcg caggcgccac acggcgtgac cgccggcgtc gctttcccac
     5881 agaccttgcg tgagccctgc cgcggccggg acggcgacgg cgtaacgcag cggtcctggt
     5941 ttgcggtaat cggcgaaggg cagggccggc gcagcgatgc tcagcgcggg cagctgtgcc
     6001 accggcgtgt tcgcatgcgc gggcagggcg gcgcacagca ggagcgcgat tgcggcgtgg
     6061 caggtgcggt gcacggcggc attgcagggg aatggtgcac ggatcatagc cgccgccgat
     6121 ggccgtcacg tgagcagaaa ccagttaccg caccggcctc tgcgatgtta ctaaatatcg
     6181 atcattttta aaaatgtacg acaaaataga tcaagccaat atgcgaggcg gtaaggcatc
     6241 gcgcgcgtgc ggtgggcaac gcccgcatgt cacgagacgc gctgcgcgtg cctggagaac
     6301 ttttccgcat gcggcggatc gaagcgcgca gaccggtgag gggagcgtac ccgtggccgc
     6361 tggcaaaaaa ttatcgataa tctataatgc gcgcccgatg gagcagtgcc gccaggacgg
     6421 agtttcacga tgtggatagt gcagatcgcg ttgcggcggc cgtacacgtt catcgtgctg
     6481 gcgctgctga tcgtgctgct cggcgggctg gccatcttca atacgcccac cgacatcttc
     6541 cccaacatca ggatcccggt ggtggccacc atctggcgct acaccggcct gccgccggaa
     6601 gacatggcca accgcgtggt gctgttctcc gagcgcaccg cgcagacggt ggtcaacgac
     6661 gtcgagcaca ccgagtcgca gtcgctcaac ggcaccgcgg tggtcaagta tttcttccag
     6721 ccgcatgcca atgaagatct gagctattcg cagatcaccg gcgtggcgca gacccagctg
     6781 gcgttctcgc cgccgggcac cacgccgccg ttcatcctgg cctacaacgc ctccagcgtg
     6841 ccgatcctgc agctggcctt caccagcgag acgctgccgg aaaaccagat cttcgaccag
     6901 gccaacagcg tggtgcgcac cgcgctggcc acggtcaacg gcgcatcgat cccattcccc
     6961 tacggcggca ggcagcgcca ggtgcaggtg gacctcgacc ccgccgcgct gcgcgcctat
     7021 gggctgacgc cgcaggacgt gtccactgcc atcggccagc agaacctgat cctgccgcag
     7081 ggcagcgaga agatcggcga cctcgagtac ttcatcaagc tcaacgccag cccgaccaag
     7141 gtcgaggagc tcaacgacct gccgatcaag acgcgcaac
//
LOCUS       DASZZC010000207         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_397355,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000207 DASZZC010000000
VERSION     DASZZC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397355"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..495
                     /locus_tag="VHE37_04505"
     CDS             190..495
                     /locus_tag="VHE37_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34815.1"
                     /translation="MTKHITTAAIFAFSAALGLAACSHNKEKPDTTASADTSMPAPAP
                     ATASNDTSNSGSTLASSTGTDTGASAMSPTTDVAAMEAQPDQSSNAQSQNYEKSNRK"
     gene            complement(394..1503)
                     /locus_tag="VHE37_04510"
     CDS             complement(394..1503)
                     /locus_tag="VHE37_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVT34816.1"
                     /translation="MTPDSLLRWRRLCACLLLAGAARAQAAVFPLPPPDQDLVGVPTW
                     INAQPGDTLATIAFRQGLGFREVIHANPGVDPWKLRTGQRIALPTQLLLPDAPRVGIV
                     VNVPEMRLYYFPKDDAHVFVYPVAVGRVDWKTPLGDTVVRKKVANPTWHPTDSIRAEH
                     AAQGDPLPLEVPPGEDNPLGAYALRLGWPDILIHGTDKPMGGIGMPVTHGCIRLYPQD
                     IETLYNVVPVGTPVSVIEQAYKLGRGGTHWYVEARAGVSADPQAPMGTAEIEDALATL
                     KARADDEQITPLDWVAIQKILRRGDGLPALAEPRPPMPLRLALHQPQAAAPRWVLRSR
                     PQLLAVGLLVVLALGVAGLVRLRFHRRDVGGGAHG"
BASE COUNT          288 a          609 c          587 g          283 t
ORIGIN      
        1 ttcctggtgt tcaccgccgc gcgccgcagg cgctggtgcg gacgctgcga cgcaggccga
       61 agtgaaaaaa tttctggcat gccggcacgc gcgaacccgc gcctgcgcca gccggtcgta
      121 ctgacgtagc ggctgcctca cggatatgtc ttgggcaccg cgttcatcca tacgaaccag
      181 gaactgacca tgaccaagca catcactact gcggcgatct tcgccttctc ggcagccctc
      241 gggctggcgg cgtgctcgca caacaaggag aagccggaca ccaccgcgtc cgccgacacc
      301 tcgatgccgg cgccggcccc ggccaccgca tcgaatgaca ccagcaacag cggcagcacg
      361 ctggcctcgt ccaccggcac cgacaccggc gcgtcagcca tgagccccac caccgacgtc
      421 gcggcgatgg aagcgcagcc ggaccagtcc agcaacgccc agagccagaa ctacgagaag
      481 tccaaccgca agtaattgcg gcctgctccg cagcacccat cgcggtgccg ccgcctgcgg
      541 ctggtgaagt gccagccgca gcggcatggg aggacgcggt tccgccagcg ccggcagtcc
      601 atcgccccgg cgcaggatct tctggatggc cacccagtcc aggggtgtaa tttgctcatc
      661 atccgcgcgc gccttcaggg ttgccagcgc atcctcgatt tccgccgtgc ccattggcgc
      721 ctgcgggtct gcgctgacgc cggcgcgtgc ttccacgtac cagtgcgtgc cgccgcggcc
      781 gagcttgtac gcctgctcga tgacactcac cggcgtgcca accggcacca cgttgtagag
      841 cgtctcgatg tcctgcggat agaggcggat gcagccgtgc gtcaccggca tgccgatgcc
      901 ccccatgggc ttgtcggtgc cgtggatcag gatgtcgggc cagcccaggc gcagcgcata
      961 agcgccgagc gggttgtcct cgcccggcgg cacttcgagc ggcagcggat cgccttgcgc
     1021 cgcgtgctcg gcgcggatgc tgtctgtggg atgccaggtg ggattggcca ctttcttgcg
     1081 caccacggtg tcgcccagcg gcgtcttcca gtccacccgg cccaccgcga ccgggtagac
     1141 gaacacgtgt gcatcgtcct tcggaaaata gtagaggcgc atctcgggca cattgaccac
     1201 gatgcccacg cgcggcgcgt cgggcagcaa cagttgagtg ggtagggcaa tacgctggcc
     1261 ggtgcgcagc ttccacgggt cgacaccggg attggcatgg atcacctcgc ggaagcccag
     1321 tccctggcgg aaggcgatgg tggccagcgt gtcgcccggc tgcgcattga tccacgtggg
     1381 cacgccgacc aggtcctggt ccggcggcgg cagcgggaac acggcggcct gcgcgcgtgc
     1441 cgctccggcg agcagcaggc atgcgcagag tctgcgccag cggagcagcg agtcgggcgt
     1501 catgggcttg cctttggacc gggcgcagtc agcgctggtt cagcttgcgg cgagcacctt
     1561 agccaggtgc ttgccttaac tcatcttgat gttcgcgccg gagagttcgt ggcaagcttc
     1621 tgcagtcgat ccgcgccgtc gacggcgcgc agtgccgaag ggagtgccga tgaacaagtt
     1681 cctgctggcc gcggtgtgct cgggtgcggt gctgctgctc gccgcgtgcg acgactacaa
     1741 caatgactac ccgccgccgc agccgat
//
LOCUS       DASZZC010000208         6013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_917435,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000208 DASZZC010000000
VERSION     DASZZC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917435"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VHE37_04515"
     CDS             <1..256
                     /locus_tag="VHE37_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34817.1"
                     /translation="LLPAALLCACATYDPVRVDDDFGQSVHQMISNQIYDASAPGARS
                     VSAPRGIDGVQAEALLKKQREGVGNPKDVELPIDVTIQQH"
     gene            263..1465
                     /locus_tag="VHE37_04520"
     CDS             263..1465
                     /locus_tag="VHE37_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT34818.1"
                     /translation="MPTKDVVLIVSPSHIACDQLCGLLETQPAIELRRLVLGGASADE
                     MAGSQPRLVVLDLGEHPAQSLSDWNLHTRLQRVPMIVVGPANDSTVMRRAMQGGARDY
                     FTRPLQPDELGASVRQVLRETAPAGDKPVGGRLTAVINAKGGSGASLIACNVAHILAA
                     RNGRSTALIDMDLQFGALPLALDLKVRESMIDVIAAADRLDPVALKGYMTLHSSGLAV
                     LGAMTDQLAFPAEIPVEAVQTVLDVALQTFEHVVVDLPKQIDALTGMVLRRADRILVP
                     LQQSIAHLRDAKRMYTLLLNYLGVPKDRVSLVLNRYSDKNAVSVEDIDQAIQPSALLT
                     VPNDFYHVSEALNLGVPLYQGARTAPVTESLCALAAHVEGSAEAPAAESGTRSRLKRV
                     FGFAGARA"
     gene            1462..2835
                     /locus_tag="VHE37_04525"
     CDS             1462..2835
                     /locus_tag="VHE37_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HVT34819.1"
                     /translation="MKLFGARSDTAEAPAPRVPVAPAAPAAPASGWTLSPHEQEWMQR
                     IHQALLQQLDLQRLGQMDEKAARREIREEAQRRMNDQSVPLSANSRQVLAKRIEDEIL
                     GLGPLEPLLADPTVSDILVNGPGSIFVERRGKLEPTDIRFRDEAHLMTIIDRSVTRGG
                     RRIDEASPMVDARLADGSRVNAIIPPLALDGAMLSIRRFAVDRMSAEDFIRLGSLTRE
                     IADVLAAIVRGRLNVLISGGTGTGKTTLLNMLSAFIPHNERILTIEDSAELQLQQPHV
                     GRLETRPANIEGRGQVTQRDLVRNALRMRPDRIIVGEVRGGEALDMLQAMNTGHDGSL
                     TTVHANSPRDALGRIENMVAMSGVDLPVKVVRSQIASAIDIVIQAERMEDGRRRVTSV
                     QEVQGMEGEVIVMAEIFRFERRGIDADGMVIGSFGSTGMVPQFYEHLRRRGIQLDLKL
                     FQSQFDN"
     gene            2838..3800
                     /locus_tag="VHE37_04530"
     CDS             2838..3800
                     /locus_tag="VHE37_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT34820.1"
                     /translation="MHAYSWLISAIVFVAVFMAALALVSYLGGAGRRRLQQRMQQLNQ
                     AAPVEEARSLLREQYLRDLTPLERFLEALPGMRNIERLSEQSGRRVPAYRVVLLSLGL
                     AALGALVLLACGRPFLALVAAAVLLPAPYLKLLNDRNNRVTLFEEQLADALDVMSRAL
                     KAGNPFNETLKVVAQEMSDPISTEFGITFSDINFGVSVKSALLGLLERVPNISLSAMV
                     TAVLVQRETGGNMAEILERVAQILRQRHRFKRRLRTLTAEGRMSAWVLVLMPFALAVV
                     LSIVSPDYLPRMTQDPFGVQLIIGAVVMMGLGVLWIRRVIRVRY"
     gene            3811..4773
                     /locus_tag="VHE37_04535"
     CDS             3811..4773
                     /locus_tag="VHE37_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT34821.1"
                     /translation="MEQLYTLLGNHLVIGALTALIGASVMLGSFAALRAAGMRADPLR
                     RRLAELGQQVQDSVPKAAPGNLGKALQPLAPFLLPKKEEERETLRLKLIRAGFRSDAA
                     AINLYLAKMILFAALPAIVVGWLGSSTEVQPVYIMFFGAWAAFIGMFLPNLYLRHRIG
                     ARQQEMMNSLPDALDLLVSCTEAGLGLNGALQRVARQMHISSPLLAHELEQVNAEIRG
                     GVDRIVALKNLAVRTGLEEIRGLVSLLSQSLRFGTPIADILRIYAEEFRDKRSQRAEE
                     QAALVGTKLIFPLCFCIFPAFFVVAVGPAVIGVTRAMSGVHFGS"
     gene            4785..5678
                     /locus_tag="VHE37_04540"
     CDS             4785..5678
                     /locus_tag="VHE37_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT34822.1"
                     /translation="MKPSIFPLLLVAALVQGCASTAAHPAASATHARSTAAEKNAAAQ
                     TPAQLLAAGEQAERGGDLNHALLSYVDAAMADPRNAEAHYRVARVQDALGNRDTAREA
                     YERAIALNPVHAGALEGLGLLLMEAGNSDRASRLLRQSLEREPRRWRACNGLGVLADL
                     AGDHVQAQTYFSRALKSEPGRAELLNNMGYSLYLAGNLGASRDYFERALTQSPSYATA
                     WDNLALVRVRLGDSAGAVDAFSESLKPYQAYNNVGYLLYINGRLDEARGYLQKAIALS
                     PTYYAQAEENLARVDGASRAP"
BASE COUNT          955 a         2107 c         2034 g          917 t
ORIGIN      
        1 cctgctgccc gcggccctgc tgtgcgcctg cgccacctat gacccggtac gcgtcgacga
       61 cgatttcggc cagtcggtgc accagatgat cagcaaccag atctacgacg ccagcgcgcc
      121 cggcgcgcgc tcggtgtcgg cgccgcgcgg catcgacggc gtgcaggccg aggcgctgct
      181 caagaagcag cgcgaaggcg tcggcaatcc caaggatgtc gaactgccga tcgacgtcac
      241 catccagcag cactgaatcg ccatgccaac caaagacgtg gtactcatcg tcagcccctc
      301 gcacattgcc tgcgaccagc tgtgcggcct gctggaaacc cagcctgcca tcgagctgcg
      361 ccgcctggta ctgggcggcg ccagcgccga cgagatggcc ggcagccagc cgcggctggt
      421 ggtgctggac ctgggtgaac acccggcgca gtcgctgtcc gactggaacc tgcacacgcg
      481 cctgcagcgc gtgccgatga tcgtggtcgg cccggccaac gacagcacgg tgatgcgccg
      541 cgccatgcag ggcggcgcgc gcgactactt cacccgcccg ctgcagcccg atgaactggg
      601 tgcctcggta cgccaggtgc tgcgcgagac cgcgccggcc ggcgacaagc cggttggcgg
      661 ccgcctgacc gcggtgatca acgccaaggg cggctccggc gcctcgctga tcgcctgcaa
      721 cgtcgcccac atcctggctg cgcgcaacgg ccgcagcacg gcgctgatcg acatggacct
      781 gcagttcggc gcgctgccgc tggcgctgga cctgaaggtg cgcgagagca tgatcgacgt
      841 catcgccgcc gccgatcgcc tggacccggt tgcgctcaag ggctacatga cgctgcacag
      901 cagcgggctg gcggtgctcg gcgccatgac cgaccagctg gcgtttcccg cggagatccc
      961 ggtggaagcg gtgcagaccg tgctcgacgt ggcgctgcag accttcgagc acgtggtggt
     1021 ggacctgccc aagcagatcg atgcgctgac cggcatggtg ctgcgccgcg ccgaccgcat
     1081 cctggtgccg ctgcagcaga gcattgcgca cctgcgcgat gccaagcgca tgtacacgct
     1141 gctgctgaac tacctgggcg tgcccaagga ccgcgtcagc ctggtgctca accgctacag
     1201 cgacaagaac gcggtgagcg tggaagacat cgaccaggcg atccagccgt cggccctgct
     1261 caccgtgccc aacgatttct accacgtgag cgaggcgctc aacctgggcg tgccgctgta
     1321 ccagggtgcg cgcaccgcgc cggtcaccga aagcctgtgc gcgctggcgg cgcatgtgga
     1381 aggcagcgcc gaggcgccgg ccgccgagtc cggcacgcgc agccgcctga agcgcgtgtt
     1441 cggcttcgcc ggggcgcgcg cgtgaaactg ttcggcgcac gcagcgatac cgcggaggcg
     1501 ccggcgccgc gcgtgccggt tgcgcccgcg gcgccggcag ccccggccag cggctggacg
     1561 ctgagcccgc acgagcagga gtggatgcag cgcatccacc aggcgctgct gcagcagctc
     1621 gacctgcagc ggctggggca gatggacgag aaggcagcac gccgcgaaat ccgcgaggag
     1681 gcgcagcgac gcatgaacga ccaatccgtt ccgctatccg ccaattcccg ccaggtgctg
     1741 gccaaacgca tcgaggacga gatcctcggc ctcggcccgc tggagccgct gctggccgac
     1801 ccgaccgtgt ccgacatcct ggtcaacggc ccgggcagca tcttcgtcga gcgccgcggc
     1861 aagctcgagc cgaccgacat ccgcttccgc gacgaggcgc acctgatgac catcatcgac
     1921 cgcagcgtca cgcgcggggg ccggcgcatc gacgaggcct cgccgatggt cgatgcgcgc
     1981 ctggccgacg gctcgcgcgt caatgccatc atcccgccgc tggcgctgga cggcgccatg
     2041 ctgtcgatcc gccgcttcgc tgtcgaccgc atgagcgcgg aagatttcat ccgcctgggc
     2101 agcctgacgc gcgagatcgc cgacgtgctc gccgccatcg tgcgcggccg cctcaacgtg
     2161 ctgatctccg gcggcaccgg caccggcaag accacgctgc tcaacatgct gtcggccttc
     2221 atcccgcaca acgagcgcat cctcaccatc gaggattccg ccgagctgca actgcagcag
     2281 ccgcacgtgg gccgcctgga gacgcgcccg gccaacatcg aggggcgcgg ccaggtgacg
     2341 cagcgcgacc tggtgcgcaa cgcgctgcgc atgcgcccgg accgcatcat cgtcggcgag
     2401 gtgcgcggcg gcgaggcgct ggacatgctg caggcgatga acaccggcca cgacggctcg
     2461 ctgaccacgg tgcatgccaa ctcgccgcgc gacgcgctgg gccgcatcga gaacatggtg
     2521 gccatgtccg gcgtcgacct gccggtcaag gtggtgcgtt cgcagatcgc ctcggccatc
     2581 gatatcgtga tccaggccga gcgcatggag gacggccggc gccgcgtgac cagcgtgcag
     2641 gaagtgcagg gcatggaagg cgaggtcatc gtcatggccg agatcttccg cttcgagcgc
     2701 cgcggcatcg acgccgacgg catggtcatc ggcagcttcg gcagcaccgg catggttccg
     2761 cagttctacg agcacctgcg ccgccgcggc atccagctcg acctcaagct gttccagtcg
     2821 cagttcgaca actgaccatg cacgcctaca gctggctgat ttccgcgatc gtgttcgtcg
     2881 ccgtgttcat ggcggcgctg gcgctggtgt cgtacctggg cggcgccggg cgccggcgcc
     2941 tgcagcagcg catgcagcag ctcaaccagg cggcgccggt ggaggaagcg cgctcgctgc
     3001 tgcgcgagca atacctgcgc gatctaacgc cgctggagcg tttcctcgaa gcgttgcccg
     3061 gcatgcgcaa catcgagcgg ctgtcggagc agtccggccg ccgcgtgccc gcttaccggg
     3121 tggtactgct gtcgctgggc ctggccgcgc tcggtgcgct ggtgctgctg gcctgcggcc
     3181 ggccgttcct ggcgctggtc gcggccgcgg tgctgctgcc ggcgccgtac ctgaagctgc
     3241 tcaacgaccg caacaaccgg gtgacgctgt tcgaggagca gctggccgac gcgctggacg
     3301 tgatgagccg cgcgctcaag gccggcaacc cgttcaacga gacgcttaag gtggtggcgc
     3361 aggagatgag cgatcccatc tccaccgagt tcggcatcac cttttccgac atcaacttcg
     3421 gcgtcagcgt gaagtccgcc ctgctcggcc tgctcgagcg cgtgcccaac atcagcctgt
     3481 cggccatggt caccgcggtg ctggtgcagc gcgaaaccgg cggcaacatg gccgagatcc
     3541 tcgagcgcgt ggcgcagatc ctgcgccagc ggcaccgctt caagcgccgc ctgcgcacgc
     3601 tcaccgccga aggccgcatg tcggcctggg tgctggtgct gatgccgttc gcgctggccg
     3661 tggtgctgtc gatcgtttcg cccgactacc tgccgcgcat gacccaggac cccttcggcg
     3721 tccagctgat catcggcgcc gtggtcatga tgggcctggg cgtgctgtgg atccgccgcg
     3781 tcatccgcgt gcgctactga aggagccgcc atggaacagc tctacacgct gctgggcaac
     3841 cacctggtga tcggcgcgct gaccgcgctg atcggcgcat cggtgatgct cggctcgttc
     3901 gccgcgctgc gcgccgccgg catgcgcgcc gacccgctgc gccgccgcct ggcggaactg
     3961 ggccagcagg tgcaggacag cgtgccgaag gccgcgcccg gcaacctggg caaggcgctg
     4021 cagccgctgg cgccattcct gctgccgaag aaggaggagg agcgcgaaac gctgcgcctc
     4081 aagctgatcc gcgccggctt ccgctccgat gccgctgcca tcaatctcta cctggccaag
     4141 atgatcctgt tcgccgcact gccggccatc gtggtcggct ggctcggcag cagcaccgag
     4201 gtgcagccgg tctacatcat gttcttcggc gcctgggccg ccttcatcgg catgttcctg
     4261 cccaacctct acctgcggca tcgcatcggc gcacgccagc aggagatgat gaacagcctg
     4321 cccgacgcgc tcgacctgct ggtgtcgtgt accgaggccg gactgggcct caacggtgcg
     4381 ctgcagcgcg tggcgcggca gatgcacatc agcagcccgc tgctggcgca tgaacttgaa
     4441 caggtcaacg cggaaatccg cggcggcgtc gaccgcatcg tggcgctgaa gaacctggcg
     4501 gtgcgcaccg gcctggagga aatccgcggg ctggtgtcgc tgctgtcgca gagcctgcgc
     4561 ttcggcacgc cgatcgccga catcctgcgc atctacgccg aggagttccg cgacaagcgc
     4621 agccagcgtg ccgaggagca ggccgcgctg gtcggcacca agctgatctt cccgctgtgc
     4681 ttctgcatct ttcccgcctt cttcgtggtt gccgtaggcc ccgccgtgat cggcgtgacg
     4741 cgcgccatga gcggcgtgca tttcggctcc tgaggctatt tcacatgaaa ccatcaatct
     4801 ttccgctgct gctcgtcgct gccctggtcc agggttgcgc gagcacggct gcacatccgg
     4861 cagcgtcggc cacgcatgcg cgcagcactg cggcggaaaa gaacgctgcc gcgcagacgc
     4921 cggcgcagtt gctcgcggcc ggcgagcagg ccgagcgcgg gggtgatctc aaccacgcgc
     4981 tgctcagcta cgtggacgcg gcgatggccg acccgcgcaa tgccgaggcg cactaccgcg
     5041 tggcgcgcgt gcaggacgcg ctgggcaacc gcgacaccgc gcgcgaggcg tacgagcgcg
     5101 ccatcgcgct caacccggtg catgccggcg cgctggaagg gctgggcctg ctgctgatgg
     5161 aggcgggcaa cagcgaccgc gccagccgcc tgctgcgcca gtcactggag cgcgagccgc
     5221 gccgctggcg cgcgtgcaat ggcctgggcg tgctggccga cctggccggc gaccacgtgc
     5281 aggcgcagac ctatttcagc cgcgcgctca agtccgagcc gggccgggcc gaactgctca
     5341 acaacatggg ctactcgctg tacctcgccg gcaacctcgg tgcctcgcgc gattacttcg
     5401 agcgcgcgct tacgcagtcg ccgtcgtacg ccaccgcctg ggacaacctg gcgctggtgc
     5461 gcgtgcgcct gggcgacagc gcgggtgcgg tcgatgcctt cagcgaatcg ctcaagccgt
     5521 accaggccta caacaacgtg ggctacctgc tgtacatcaa cggcaggctc gacgaggcgc
     5581 gcggttacct gcagaaggcc atcgcgctgt cgccgacgta ttacgcgcag gcagaggaaa
     5641 acctggcgcg cgtcgacggc gcatcgcgcg ccccctgacg ccgcgcgcta cggaaatcgt
     5701 agcggaggat gacgacggaa gcgttgaaac tgcctcaccg caccccggtt ttgcttgtta
     5761 gtaattattg tttaattgtt aaaatagcca acatttaata acagtataaa tgccggtgcg
     5821 gtggggcgga aaaatgccgt ggcactgtct acaccggcgg cggcgcgatg ctcatcgggt
     5881 cgaagggcgg cagcggcgcg ccggtttcca gcagtgtctt cagcccgcac aggatcagcg
     5941 gccagccgcg gcgcccgcct tccttgaagc tctccggcag cgattcgtca tgggtttcgg
     6001 tcaccgacag ccg
//
LOCUS       DASZZC010000209         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_18828,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000209 DASZZC010000000
VERSION     DASZZC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18828"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VHE37_04545"
     CDS             <1..1202
                     /locus_tag="VHE37_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT34823.1"
                     /translation="KAADPGLLAVLLSAVLGGLILNLMPCVFPVLSLKALSLVAAREH
                     GDTHQRAHALAYTAGVLLSFAGVAGALLAVRAAGMAAGWGFQLQSPPMVGGLAYLMLA
                     LGLFLSGAAEFGTGWMGAGQWLAQRAGLAGSFFTGVLATLVATPCTAPFMGAALGYAI
                     TQPTVIALAVFLALGLGLALPFLLLGFFPPLARVLPRPGAWMQTFKQLMAFPLYLTSA
                     GLVWVLARQTAADGAGIVLAGMVLIAFAVWLWNRAGWFAGLLRIAAVAGALALLASPW
                     LMQAPAQPAAAQAQNQAWSAARVAELRAVGRTVFVNFTADWCITCKVNERVALDNERV
                     RTAFKQNEVVWLTGDWTRADPEITHVLEQFGRTGVPLYLVYAAGGEPKVLPQVLTPDI
                     VIDAIKP"
     gene            1215..>1568
                     /locus_tag="VHE37_04550"
     CDS             1215..>1568
                     /locus_tag="VHE37_04550"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HVT34824.1"
                     /translation="MKLIRALALLVVSTVALADVKPGDAAPAFSLPGSDGRTHALADY
                     AGKFVVLEWTNDGCPFVHKHYDSGNMQSLQKDATARGVVWLSMVSSAPGKEGHVTAQQ
                     AAQLTRERHAAPTAVL"
BASE COUNT          202 a          541 c          568 g          257 t
ORIGIN      
        1 agaaagccgc cgacccggga ttgctggcgg tgctgctctc ggcggtactc ggcgggctga
       61 tcctgaacct gatgccctgc gtgttcccgg tgctgtcgct caaggcactg tcgctggttg
      121 ccgcgcgcga gcacggcgac acgcaccagc gcgcacacgc gctggcctac accgccggcg
      181 tgctgctcag ctttgccggc gtggccggcg cgctgctcgc ggtgcgcgcg gccggcatgg
      241 ccgcgggctg gggcttccaa ctgcaatcgc cgccgatggt cggcggactc gcttacctga
      301 tgcttgcact ggggctgttc ctgtccggcg ctgccgagtt cggcaccggc tggatgggcg
      361 ccggccagtg gctggcgcag cgcgcgggcc tggccggctc gttcttcacc ggtgtgctgg
      421 ccacgctggt ggcgacgccg tgcacggcgc cgttcatggg cgcggcgttg ggctacgcca
      481 tcacgcagcc gacggtcatc gcgctcgccg tgtttctcgc gctgggcctg ggactggcgc
      541 tgccgttcct gctgctcggc ttcttcccgc cgctggcgcg cgtgctgccg cggcccggcg
      601 cgtggatgca gaccttcaag cagctgatgg ccttcccgct gtatctcacc agcgccgggc
      661 tggtgtgggt gctggcgcgg cagacggcag ccgacggcgc gggcatcgtg ctcgccggca
      721 tggtgctgat cgcattcgcc gtgtggctgt ggaaccgcgc cggctggttt gccgggctgc
      781 tgcgcatcgc ggccgtcgcc ggcgcgctgg ccctgctcgc cagcccgtgg ctgatgcagg
      841 caccggcgca gccggccgcc gcgcaggcgc aaaaccaggc ctggtccgcg gcgcgcgtgg
      901 cggaactgcg cgcggtggga cgcacggtgt tcgtcaactt caccgccgac tggtgcatca
      961 cctgcaaggt caacgagcgc gtggcgctgg acaacgagcg cgtgcgcacg gcgttcaagc
     1021 agaacgaagt cgtatggctc accggcgact ggacgcgcgc cgacccggag atcacgcatg
     1081 tactggagca gttcggccgc accggcgtgc cgctgtacct ggtctatgcc gcgggcgggg
     1141 aaccgaaggt gctgccgcag gttctcactc ctgacatcgt catcgacgcg atcaagccct
     1201 aacggagccg tctcatgaaa ctcatccgcg cgctcgcact gctggtcgtc tcgactgtcg
     1261 cgctggccga cgtgaagccc ggcgacgctg cgccggcgtt ctcgctgccc ggcagcgacg
     1321 gcaggacgca tgcactggcg gattacgccg gcaaattcgt ggtgctggag tggaccaacg
     1381 atggctgccc gttcgtgcac aagcattacg acagcggcaa catgcagtcg ctgcagaagg
     1441 acgccaccgc gcgcggcgtg gtctggctga gcatggtgtc gtcggcgccg ggcaaggaag
     1501 gccacgtgac ggcgcagcag gcggcgcagc tcacacgcga gcgccacgcc gcgcccaccg
     1561 cagtgctg
//
LOCUS       DASZZC010000210         8154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_530373,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000210 DASZZC010000000
VERSION     DASZZC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530373"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VHE37_04555"
     CDS             complement(<1..187)
                     /locus_tag="VHE37_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVT34825.1"
                     /translation="MFLPDRYIKGECPNCGTADQYGDNCENCGATYSPRDLKNPFSVL
                     SGAAPVWKPSEHYFFAAS"
     gene            212..1951
                     /gene="metG"
                     /locus_tag="VHE37_04560"
     CDS             212..1951
                     /gene="metG"
                     /locus_tag="VHE37_04560"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HVT34826.1"
                     /translation="MAFDNYYSTHSPENRELSELIYARLSQGGFIARRTIQQLYDPVK
                     NMFLPDRYIKGECPRCGTPDQYGDNCENCGAVYAPTDLKNPRSVVSGAPPVMKDSEHF
                     FFELSKFQQFLSGWLEGDVAHPSVKAKLREWFAAGLKDWDISRDAPYFGFAIPNEPGK
                     YFYVWLDAPIGYMASAKNLCARSQRDFETLWRDSEIHHFIGKDIINFHGLFWPAMLHG
                     SQFRVPAKLHVNGYLTINGQKMSKSRGTFVRARTYLNHLNPEYLRYYFAAKLGAGVDD
                     LDLSLEDFAARINSDLVGKFVNIASRCAGFIEKNFGGKLAAQLYQGEAFLFRDFADEA
                     ANLAALYEAGSYSDALREIMLMADDANAEIQKLAPWKLARDPAQLEMLHQVCTTFINL
                     FRQLAIYLKPVLPATATKIEALLGLPAQGWDDAQRPLLAHSIKPYEALITRIEGKTVE
                     AMMAEEAASTTTAPAPVPATPPISIDDFGKVELRIARIEKAESVEGADKLLRLTLDVG
                     ELGTRQVFAGIKSAYAPETLTGRLTVVVANLAPRKMKFGTSSGMVLAASSDAGGPFLL
                     SPDAGAQPGMKIK"
     gene            2067..2714
                     /locus_tag="VHE37_04565"
     CDS             2067..2714
                     /locus_tag="VHE37_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HVT34827.1"
                     /translation="MSSAAATPLADRIDALLPQTQCTRCGYPDCRSYAEAIAGQRADF
                     NQCPPGGDEGIAQLSALLGRAPKPLNPANGIAQVVAPVAVIDEARCIGCTLCIQACPV
                     DAISGANLLMHTVIAAACTGCELCIPPCPVDCISLETPPVTPPAWTYMRMAADEQRAR
                     YYARNARNARERQQQLDARARRKAMLRARAASSDPSEAPHEHDPLTSALARERGR"
     gene            2711..3346
                     /gene="nth"
                     /locus_tag="VHE37_04570"
     CDS             2711..3346
                     /gene="nth"
                     /locus_tag="VHE37_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVT34828.1"
                     /translation="MNSAKRRAIFETLRANNPAPTTELKYSTPFELLVAVVLSAQATD
                     VGVNKATARLYPVANTPRAILDLGETRLREYIKTIGLYQTKAKNVIGLCRQLLEQHGG
                     EVPRTREALEALPGVGRKTANVVLNTAFGEHTIAVDTHIFRVANRTGLAPGKNVREVE
                     DRLLKFTPPEFMRDAHHWLILHGRYTCKARVPECPRCVIHQWCEYRHKTVD"
     gene            complement(3510..4655)
                     /gene="ald"
                     /locus_tag="VHE37_04575"
     CDS             complement(3510..4655)
                     /gene="ald"
                     /locus_tag="VHE37_04575"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HVT34829.1"
                     /translation="MLIGTPREIKNHEYRVGLTPAGVRELCARKHQVLIQAGAGLGIG
                     LADEAYRAAGAEIVEKAEEVFARADMVVKVKEPQPVECKMLREGQLLFTYLHLAPDPE
                     QTKGLVESGCVAIAYETVTDGRGGLPLLAPMSEVAGRMSIQAGAHALEKAQGGNGVLM
                     GGVPGVAPAEVTVIGGGVVGYNAARVAVGMGANVTILDRSLPRLNWLDTTFDGRLTTL
                     YSTADALERCVSSADLVIGAVLVPGAAAPKLVTRAMLKLMKPGAVIVDVAIDQGGCFE
                     TSRPTTHQAPTYTVEGIVHYCVANMPGGVARTSTFALTNATLPYVLALADKGYRKALL
                     DDANLREGLNVHAGKVTYKAVADALGYEYQSPQEALKPWWAPNPDAH"
     gene            complement(4787..8005)
                     /locus_tag="VHE37_04580"
     CDS             complement(4787..8005)
                     /locus_tag="VHE37_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVT34830.1"
                     /translation="MRTRQNLKFLVATVFSLALTACGGGGLNFGGGTGSTTGSTTGST
                     TGSTTGGTTGGTTTQIPATLTITSAQSQITADGTSTATIKGILQDTSGNAISGTALTF
                     KTTTGKVAPAGTATFGSSATATTDSTGSAFVSLQSPTSVGSTTVVVTDPVSGLSNTID
                     VGFVAGPAATTTLKAAPSKVSPGGQASISATVVDANNNPVPNATVAFDIATNNSGGAY
                     GTTLVLTGTNGKASTIYTAGPTPSTDTLSACASGICAAGGAALQVVAGTTPIGSILVS
                     VGSSTAVANGTSKTAVTITVNDGSGHPLPSQGVNISTSAGLLDDSVDPPAATVSVNTD
                     NSGQIHLSQVSPTLVGTTAITADAGGVSGSNSINFVAGPVKTIILSSNTVTVAQGGTA
                     VLRAVALDKNNNPVPGISISFSSNELSNGASFAPAVGPTDGNGSIQTSYTAPTGTPTT
                     DDVMAHAAGNGTTIDSNKVTFTITASSTVTPAALTLIASNLTLSSSASDVSAGVILTA
                     LVRDANHNAVTGQAVQFTIPSADSGLISGSPATTDGSGKASATLTTDGDPTNRIIHVH
                     AKIPSAGLDKEIDIQVTGTTLSVTGEDTAQIGVSKTYLITLIDAASNGIGNTQVTVST
                     QAGNQLDNGTNMQTYTTGSNGTVLINLTPTVAATTISASALGLVATKSVTVSTDQFTF
                     QAPATNAEIDIGAATSVAVQWLQGGAPVADGTQIDFQTTRGTLSSPSGLTFAVGPLGA
                     ALQPKKHGVAYTSISSTEAGPAVIAANSTASTKPSTTLPMEFVATTPAVIDVQVSPAV
                     IAINQSSSLTAVVRDANNNLVKNVTVDWSLQDLSTGSLSTPSGVTNSQGRTSVTFQAS
                     SQPSSGQGVKIIATVRSNSAITNTATLTVGQKAVSIVIGTGAELGIKDTSTYQMPFTV
                     VVTDAASNPVPNATFSLTLLPHSFFKGDFINGVEECPNEDLNFNDIVDPGEDNDGDNL
                     LEPGRVASIPSTITLGSDGAGQFNITYPKDHGQFVSVEIIGTVSDVSGDQATTTRVVT
                     LPIAQKDAQNLPGVSPYGTDISNGPTVFHGTTGILEDCFNPN"
BASE COUNT         1386 a         2630 c         2704 g         1434 t
ORIGIN      
        1 tggacgccgc gaagaaatag tgttccgacg gcttccagac gggcgcggcg cccgacagca
       61 ccgagaacgg attcttcagg tcgcgcggcg agtaggtggc gccgcagttc tcgcagttgt
      121 cgccgtactg gtcggcggtg ccgcagttcg ggcattcgcc cttgatgtag cgatcgggca
      181 ggaacatgca cgcgacttcg ccgacttcgg cgtggccttc gacaactact actcgacgca
      241 ctcgccggaa aaccgcgagc tgtcggagct gatctacgcg cgcctgtcgc agggcggctt
      301 catcgcgcgg cgcaccatcc agcagctgta cgacccggtg aagaacatgt ttcttcccga
      361 ccgctacatc aagggcgagt gtccgcgctg cggcacgccc gaccagtacg gcgacaactg
      421 cgagaactgc ggggcggtct acgcgcccac cgacctgaag aatccgcgct cggtggtctc
      481 cggcgcgccg ccggtgatga aggactccga gcatttcttc ttcgagctgt cgaagttcca
      541 gcagttcctc agcggctggc tggaaggcga cgtggcgcat ccctcggtca aggccaagct
      601 gcgcgagtgg ttcgccgccg ggctgaagga ctgggacatc tcgcgcgacg cgccgtactt
      661 cggcttcgcc attcccaacg agccgggcaa gtatttctac gtgtggctgg acgcgccgat
      721 cggctacatg gccagcgcga agaacctctg cgcgcgcagc cagcgcgatt tcgagacgct
      781 gtggcgcgac agcgagatcc accacttcat cggcaaggac atcatcaact tccacggcct
      841 gttctggccg gcgatgctgc acggctcgca gttccgcgtg ccggccaagc tgcacgtcaa
      901 cggctacctg accatcaatg gccagaagat gagcaagtcg cgcggcacct tcgtgcgcgc
      961 gcgcacctat ctcaaccacc tgaacccgga atacctgcgc tactacttcg cggccaagct
     1021 cggcgcgggc gtggacgacc tcgatctcag cctggaggac tttgccgcgc gcatcaacag
     1081 cgacctggtc ggcaagttcg tcaacatcgc cagccgctgc gccggtttta tcgagaagaa
     1141 tttcggcggc aagctcgctg cgcagctgta ccagggcgaa gccttcctgt ttcgcgattt
     1201 tgccgacgag gcggccaacc ttgccgcgct gtacgaggcc ggctcctaca gcgacgcgct
     1261 gcgcgagatc atgctgatgg ccgacgacgc caacgcggaa atccagaagc tggcgccgtg
     1321 gaagctggcg cgcgatccgg cgcagctgga gatgctgcac caggtgtgca ccaccttcat
     1381 taacctgttc cgccagttgg ccatctacct caagccggtg ctgccggcga ctgctacgaa
     1441 aatcgaagcg ctgctcggcc tgccggcgca gggttgggac gatgcccagc ggccgctgct
     1501 ggcacacagc atcaagccgt acgaggcgct gatcacgcgc atcgagggca aaaccgtgga
     1561 ggccatgatg gccgaggaag ctgcatcgac caccaccgcg cccgcaccgg tgccggccac
     1621 gccgccgatc tcgatcgacg atttcggcaa ggtggagctg cgcattgcgc gcatcgagaa
     1681 agccgagagc gtggaaggcg ccgacaagct gctgcgcctg acgctggacg tcggcgagct
     1741 gggcacgcgc caggtgtttg ccggcatcaa gtccgcgtat gcacccgaga cgctcaccgg
     1801 ccgccttacc gtggtggtgg ccaatctcgc gccccgcaag atgaagttcg gcaccagctc
     1861 cggcatggtg ctggccgcaa gctccgacgc cggcggcccg ttcctgctgt cgcccgatgc
     1921 cggcgcgcag ccgggcatga agatcaagtg atgtattccg ctgcatcgct gcgggatacg
     1981 tcatctccgc gcaggcggac atccagtttt cccctcacga aaaagcaaga ctgggctccc
     2041 gcctgcgcgc gaacgacgga ctggtcttga gcagcgctgc agcgacgccg ctggccgatc
     2101 gcatcgacgc gctgctgccg cagacgcaat gcacgcgctg cggctatccc gattgccgca
     2161 gctatgcgga ggcgattgcc gggcagcgtg ctgatttcaa ccagtgcccg cccggaggcg
     2221 acgaaggcat cgcgcagctt tccgcgctgc tcggccgcgc gcctaagccg ctcaatccgg
     2281 ccaacggcat cgcgcaggtg gtggcgccgg tggcggtaat cgacgaagcg cgctgcatcg
     2341 gctgcacgct gtgcatccag gcctgcccgg tcgacgcgat cagcggcgcc aacctgctga
     2401 tgcacacggt gatcgccgcg gcctgcaccg gctgcgagct gtgcatcccg ccctgcccgg
     2461 tcgattgcat ttcgctggag acgccgccgg tgacgccgcc cgcatggacg tacatgcgca
     2521 tggccgcaga cgagcagcgc gcgcgttact acgcgcgcaa tgcgcgcaac gcacgcgagc
     2581 gtcagcagca actggacgca cgggcgcggc gcaaggccat gctgcgcgcg cgggcggcga
     2641 gcagcgatcc ttccgaggcg ccgcacgagc acgatccgct cacctctgcg ctggcgcgcg
     2701 agcgcggccg atgaattccg ccaagcgccg cgccatcttc gaaacgctgc gcgcaaacaa
     2761 cccggcgccg accaccgaac tgaaatactc gacgccgttc gagctgctgg tggccgtggt
     2821 gctgtcggcg caggccaccg acgtgggcgt gaacaaggcg acggccaggc tctatcccgt
     2881 cgccaacacg ccgcgcgcga tcctcgacct gggcgaaacc aggctgcgcg agtacatcaa
     2941 gacgatcggc ctgtaccaga ccaaggcgaa gaacgtgatc ggcctgtgca ggcaactgct
     3001 ggaacagcac ggcggcgaag tgccgcgcac gcgcgaggcg ctggaagcgt tgccgggcgt
     3061 cggccgcaag accgccaacg tggtgctcaa caccgcgttc ggcgaacaca ccatagcggt
     3121 cgacacgcat atcttccgtg tggccaaccg caccggcctg gcgccgggca agaacgtgcg
     3181 cgaagtggaa gaccgcctgc tgaagttcac gccgccggag ttcatgcgcg acgcgcatca
     3241 ctggctgatc ctgcacgggc gctacacctg caaggcgcgc gtgccggaat gcccgcgctg
     3301 cgtgatccac caatggtgcg agtaccggca caagacggtg gattgagccc gcgcacggtc
     3361 caccgcactg ccgtctgtga tgttattaat tattgattga ttgttaaaac atcaaataaa
     3421 taaaaactcg caacgagggg tgcggtggca gcaaagaaac ctgccgtggc cggaaaaacg
     3481 ggcgatcagc tgctcgcagc ggatcaggat caatgcgcgt cgggattggg cgcccaccac
     3541 ggcttcagcg cttcctgcgg cgactggtac tcgtagccca gcgcgtcggc caccgccttg
     3601 taggtgacct tgccggcgtg cacgttgagg ccttcgcgca ggttggcgtc gtcgagcagc
     3661 gccttgcggt aacccttgtc ggccagtgcc agcacgtacg gcagcgtggc gttggtcagc
     3721 gcgaaagtcg acgtgcgcgc cacgccgccc ggcatgttgg ccacgcagta atgcacgatg
     3781 ccttcgacgg tataggttgg cgcctgatgc gtggtggggc gcgaggtctc gaagcagcca
     3841 ccctggtcga tggcgacatc gacgatcacc gcgcccggct tcatcagctt gagcatggcg
     3901 cgcgtcacca gcttgggcgc cgcggcaccg ggcaccagca ccgcgccgat caccaggtcg
     3961 gcgctgctca cgcaacgctc cagcgcgtcg gcggtggagt acagcgtggt caggcggccg
     4021 tcgaaggtcg tatcgagcca gttcaggcgc ggcagcgagc ggtcgaggat ggtgacgttg
     4081 gcgcccatgc ccacggcgac gcgcgcggcg ttgtagccga ccacgccgcc gccgatcacg
     4141 gtgacttccg ccggcgccac gccgggcacg ccacccatca gcacgccgtt gccaccctgc
     4201 gccttctcca gcgcatgcgc gccggcctgg atcgacatgc ggcccgccac ttcgctcatc
     4261 ggcgccagca gcggcaggcc gccgcggcca tcggtgacgg tttcataggc gatggcgacg
     4321 caaccggatt cgaccaggcc cttggtctgc tcgggatcgg gcgccaggtg caggtaggtg
     4381 aacagcagct gcccttcgcg cagcatcttg cattcgaccg gctgcggctc cttgaccttg
     4441 acgaccatgt ccgcgcgcgc gaagacctct tcggcctttt ccacgatctc ggcgccggcg
     4501 gcgcgatagg cctcatcggc cagcccgatg cccagccccg cgccggcctg gatcagcacc
     4561 tggtgcttgc gcgcgcacag ctcgcgcacg ccagccggcg tgaggccgac acggtattcg
     4621 tgatttttta tttccctggg cgtgccgatg agcatgcgtg cgctccgcaa tttggctgag
     4681 acgattatcc cacaggcgcc ggaaatgccc gccggcgcat ggcggcagcg cccaataaca
     4741 aaggcggcgc ggaaaccccg cgccgccttg aatcggtctc ccggcttcag ttggggttga
     4801 agcaatcctc caggatgccg gtggtgccgt ggaacacagt cggcccgttg ctgatgtccg
     4861 tgccgtaggg gctgacgccg gggaggttct gcgcatcctt ctgcgcaatc ggcagcgtga
     4921 ccacgcgggt ggtcgtcgcc tggtcgcccg acacgtcgga aaccgtgccg atgatttcca
     4981 cgctgacgaa ctggccgtga tccttcggat aggtgatgtt gaactgcccg gcaccgtcgc
     5041 tgcccagggt gatggtcgac ggaatcgacg ccacgcggcc cggctcgagc aggttgtcac
     5101 cgtcgttgtc ttcccctgga tcgacgatgt cgttgaaatt caggtcttca ttggggcact
     5161 cctcgacgcc attgatgaag tcgcccttga agaacgagtg cggcagcaag gtcaggctga
     5221 aggttgcgtt gggcaccggg ttgctggccg catctgtcac taccacggtg aacggcatct
     5281 ggtaggtcga agtgtccttg atccccagtt cggcgcccgt accgatgacg atgctgaccg
     5341 ccttctgtcc gacggtgagg gtcgccgtat tggtgattgc cgaattgctg cgcacggtgg
     5401 cgatgatctt gacgccctgg cccgaactcg gctgcgaact ggcctggaag gtgaccgagg
     5461 tacggccctg gctgttggtc acgcccgagg gcgtgctcag gctgccggtg gacagatcct
     5521 gcagtgacca gtcgaccgtc acgttcttca ccaggttgtt gttggcatcg cgcaccactg
     5581 ccgtgaggct gctgctctgg ttgatggcaa tcaccgccgg gctcacctgc acgtcgatta
     5641 cggcgggcgt ggtggcaacg aattccatcg gcagcgtggt gctcggcttg gtcgacgccg
     5701 tgctgttggc ggcgatcacc gccgggcctg cctcggtgga ggaaatcgag gtgtaagcca
     5761 cgccgtgctt cttgggctgg agcgccgcgc ccagcggacc gactgcgaag gtcaggccag
     5821 acggagacga cagtgtcccg cgggtggtct ggaagtcgat ctgcgtgccg tcagccaccg
     5881 gcgcgccgcc ctgcagccat tgcacggcaa ccgacgttgc agcgccgatg tcgatttctg
     5941 cattggtcgc cggcgcctgg aaggtgaatt ggtcggtaga gacggtcacg ctcttggttg
     6001 ccaccagccc cagcgccgat gccgagatgg tggtcgcggc caccgtcgga gtcaggttga
     6061 tcagcacggt gccgttggaa ccggtggtgt atgtctgcat gttggtgccg ttgtccagct
     6121 ggttgccggc ctgggtgctg accgtcacct gcgtattgcc gatgccgttg gacgcggcat
     6181 cgatcaacgt gatcaggtag gtcttgctga cgccgatctg cgcggtgtct tcgccggtaa
     6241 cggacaaggt cgtgcccgtg acctggatat cgatttcctt gtccaggcca gccgagggga
     6301 tcttggcgtg cacgtggatg atgcgattgg tcgggtcgcc gtcggtggtc agcgtggccg
     6361 acgccttgcc gctgccatcg gtggtcgctg gcgaaccgct gatcaggccc gaatccgcgc
     6421 tcggaatggt gaactgcact gcctggccgg tcaccgcgtt gtggttcgca tcgcgcacca
     6481 gtgcggtcag gatcacgccc gcgcttacgt ccgaagcgct cgacgacaag gtcagattgg
     6541 acgcaatcag cgtcagcgcg gccggcgtca ccgtggaaga agccgtgatg gtgaaggtga
     6601 ccttgttgga atcgatcgtc gtaccgttgc cggcggcgtg ggccatgaca tcgtcggtgg
     6661 tgggcgtgcc ggtgggtgcg gtgtagctgg tctggatgga accgttgccg tcggtcgggc
     6721 cgaccgcagg tgcgaacgat gcgccattgc tcagctcgtt cgaactgaag ctgatgctga
     6781 tcccgggcac ggggttgttg ttcttgtcca gcgccaccgc gcgcagcacc gccgtgccgc
     6841 cctgggccac cgtcaccgta ttgctgctca ggatgatggt cttgaccggg ccagcgacaa
     6901 aattgatgga attgctgccc gaaacgccgc cggcgtcggc agtaatagcc gttgttccca
     6961 ccagcgtggg tgacacctgg ctgagatgga tctgtccgct gttgtcggta ttgaccgaaa
     7021 cggtcgcagc cggcgggtca accgagtcat cgagcaagcc cgccgaggtg ctgatgttca
     7081 cgccctggct gggcagcgga tgaccggacc cgtcattgac cgtgatggtc accgcggtct
     7141 tgctggtgcc gttggccacc gccgtgctgc tgccaacgct gaccaaaatg gagccgatcg
     7201 gtgtcgtgcc ggcgaccacc tgcagcgccg caccgcccgc cgcgcagatg ccggaggcgc
     7261 atgcgctcaa tgtgtcggtc gaaggcgtgg gacccgccgt gtagatggta ctcgccttgc
     7321 cattggttcc cgtcagaacc agcgtcgtgc cgtatgcccc gccgctgttg ttggtggcga
     7381 tgtcgaaggc caccgtggca ttgggaaccg gattgttgtt ggcatcgacc acggtggcac
     7441 tgatcgaggc ctggccgcct ggcgacacct tggacggtgc agccttcagc gttgtggtcg
     7501 cagcaggtcc agccacgaag ccgacatcaa tcgtgttgga cagaccgctg acagggtcgg
     7561 ttaccaccac agtagtgctg ccgaccgagg tcggcgactg cagcgagaca aacgcgctgc
     7621 cggtcgagtc ggtcgtcgcc gtcgcgctcg aaccaaaggt cgcggtccct gccggggcca
     7681 ccttgccggt ggtggtcttg aaggtcagcg cggtgccgga aatggcattg cccgaagtgt
     7741 cctgcaggat gcccttgatg gtggcggtcg acgtaccgtc ggccgtgatc tgcgattgcg
     7801 ccgaggtgat ggtcagcgtc gccggaatct gcgtggtggt gccgccggtc gtgccaccgg
     7861 tggtgctccc cgtggtcgag cccgtcgtgc tgccggtggt cgagccggtg cctccaccaa
     7921 agttcaggcc gccgccgccg catgccgtga gcgcgagcga aaacactgtg gccaccagaa
     7981 acttgagatt ctgccgagta cgcataccct ttcccccgtc gctgcacgct ctgtgcagga
     8041 ttcaaaaatt attgaagatg ctaacgccaa aacctaacga aaacaagtaa atcgagaaac
     8101 aagtaaaagc atgttgcgaa gttgctggcg gagccgccgc gcggcctccc ggga
//
LOCUS       DASZZC010000211         5928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_917750,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000211 DASZZC010000000
VERSION     DASZZC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917750"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VHE37_04585"
     CDS             complement(<1..670)
                     /locus_tag="VHE37_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT34831.1"
                     /translation="MALTLESSTELCALLSDSSRLRLLMLVEAHELSVAELTEITGLA
                     QSRVSTHLARLKRAGLVIDQRAGNAAIYSATENDGAAAELWQLLRGQLDDAQTRLDHE
                     RAEQVIRARKHGQTWAESVAGRMDLHYSPGRTWEATARALIGLLSLGDVLDLASGDGV
                     LAELIAEHARSVTCIDISPTVIAAARKRLARFANVKVMEGDMHALDLPAHGYDHIFAM
                     HALSY"
     gene            complement(841..1380)
                     /locus_tag="VHE37_04590"
     CDS             complement(841..1380)
                     /locus_tag="VHE37_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34832.1"
                     /translation="MKKSRKPAAKAAKPNRLAKLKATAKKALAKVKASAKKATTKAKK
                     STSKAKASVKKVQSKAKAAVKKPVAKAKKAAAKPVAKAKKAVAKPVAKAKEAAEKAQA
                     TVQKTVAAATTRMTTLAMPVVQAVHNITPSPMSRAFLATTAGQKEAAKMEPPPPPAPE
                     AAAPAEPETPPEAGESSSF"
     gene            1694..3859
                     /gene="fadB"
                     /locus_tag="VHE37_04595"
     CDS             1694..3859
                     /gene="fadB"
                     /locus_tag="VHE37_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HVT34833.1"
                     /translation="MIQAIFEGQSLKVTLLDGGIAELCFDRQNDSINKFDARTVNELR
                     EAADKLQNAGNVKGLLVTSAKDVFIVGADITEFGQNFTRTEEEIAAWGVEANKVFSAV
                     EDLPFPSVVAINGFALGGGFEMCLSVDYRVMSTAAQIGFPEVKLGIYPGFGGTVRAPR
                     LMGADNAIEWIAGGENQKPDTALKLHAVDAVTAPDKLRAAALKLLGQAIEGKYDWKAR
                     RAQKTGRLKLDQIEAMMVFTSAKGFIAGKAGKNYPAPLAAVSAIEKAAGKTRDDAIKI
                     EAAGFAKVAKTPQADALIGLFLNDQFLKKKAKGQGKNATKPVKQAAVLGAGIMGGGIA
                     YQSAVKGTPIIMKDIKDEQLHLGMSEANKLLSKMVERKKLTPEKAGDVLSNIRATLNY
                     GDFQGVDIVVEAVVENPKVKKSVLAEVEGLVKPDTIIASNTSSISIDELSTAMKRPEN
                     FLGMHFFNPVHKMPLVEVIYGAKTSKAAIGSVVAYASAMGKTPIVVKNCPGFLVNRIL
                     FPYFGGFMGVLRDGADFVKVDKVMENFGWPMGPAYLQDVVGMDTSHHVGDVLAEGYPD
                     RMGKEFKSALDVMYENKRYGQKNGVGFYKYENDPKGKPKKIADPTSYELLKSVQPNGQ
                     KDMSDEEIIDRLMLPMIIETARCLDEGIVESPQEADMGLILGIGFPPFRGGALKYADQ
                     VGMKTVLEKAAKYEKLGKLYHPTDSMKKMAAEGKTYYSK"
     gene            3873..5033
                     /gene="fadA"
                     /locus_tag="VHE37_04600"
     CDS             3873..5033
                     /gene="fadA"
                     /locus_tag="VHE37_04600"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HVT34834.1"
                     /translation="MTDVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLIRTILQRNAA
                     VKPADVEDVIWGCVQQTLEQGFNIARNAALLAGLPQSVSGQTVNRLCGSSMTAIHAAV
                     GSIKAGVGDTFICGGVEHMGHVPMTLNFDGDPALSLSVAKASAMMGLTAEMLTQTHQV
                     GRKEQDEFALESHKRAYNAWKTGQFKNEMVPVEGHDQDGAPILVDFDEVVRSDASIEG
                     LNSLKPVFNPKGSVTAGNSSAISDGASCTLIMNADKAKALGLKPLAKIVSMASAGCNP
                     SIMGIGPVPASKKALTRAGLDLKNIDYFELNEAFAAQSLAVMKELKILDRMDRVNIKG
                     GAIALGHPLGCSGARITGALAHVLNEKNAQFGLATMCIGMGQGVATVLERVH"
     gene            5175..5687
                     /locus_tag="VHE37_04605"
     CDS             5175..5687
                     /locus_tag="VHE37_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HVT34835.1"
                     /translation="MIWVDYCILGVTLVSIIIGAFRGVVREVLGLLTWAVAFLAAALF
                     HNEVAGLLQSKISNPALCTAAGWAGTFLAGLLVGAIISSVLVEAVRNSTFSGADRTIG
                     AGFGLLRGLLLTAIFLLVAGSMGEKKSEWWQRSLFVSKLEWLADGLGMVVPQSWLEKL
                     NPDAAPTKGS"
     gene            5691..>5928
                     /locus_tag="VHE37_04610"
     CDS             5691..>5928
                     /locus_tag="VHE37_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HVT34836.1"
                     /translation="MCGIAGIVTQGSAVNSELFDALTMLQHRGQDAAGIATSGDGELC
                     VRKGSGLVRDVFQQHHMIELRGNLGIGHVRYPTAG"
BASE COUNT         1076 a         1850 c         1994 g         1008 t
ORIGIN      
        1 tgtacgacag cgcgtgcatg gcgaagatgt gatcgtagcc gtgcgccggc aggtccagcg
       61 cgtgcatgtc gccttccatc accttgacgt tggcgaagcg cgccaggcgc ttgcgcgcgg
      121 ccgcgatcac cgtggggctg atgtcgatgc aggtcacgct gcgcgcgtgc tcggcgatca
      181 gctcggccag cacgccatcg ccggacgcga gatcgagcac atcgcccagc gagagcaggc
      241 cgatcagcgc gcgcgcggtg gcttcccagg tgcggccggg cgagtagtgc aggtccatgc
      301 ggccggcgac cgactcggcc caggtctggc cgtgcttgcg cgcgcggatc acctgctcgg
      361 cgcgctcgtg gtccaggcgc gtctgcgcat cgtccagctg gccgcgcagc agctgccaaa
      421 gttcagcggc ggcgccgtcg ttctcggtgg cggaatagat cgccgcgttg ccggcacgct
      481 ggtcgatcac caggcccgcg cgcttgagcc gcgccaggtg cgtggacacg cggctctgcg
      541 ccaggccggt gatctcggtc agctcggcga cggagagctc atgcgcttcc accagcatca
      601 gcaggcgcag gcgactggag tcggacagca gggcgcagag ttcggtactg ctttcgagcg
      661 tgagagccac ctaccgcacc ccggttttgc gggttattaa atataacatg aaaatataat
      721 cagataataa ttaataacat caaaaatggc gatgcggtgg aggtcattgt gccgtgccgg
      781 acgagcgtgc gggcacaaaa aagcccgccg ggtgttccgg cgggccgttg cagcgtcgac
      841 tcagaacgag gacgactcgc cggcttccgg cggggtctcg ggctccgcag gcgcggcggc
      901 ttccggcgcg ggcggcggcg gcggttccat cttggccgcc tccttctggc cggcggtggt
      961 ggcgagaaac gcgcgcgaca tcggcgaggg cgtgatgttg tgcacggcct gcaccaccgg
     1021 catggccagc gtggtcatgc gcgtggtggc ggcggctaca gtcttctgca cggtggcctg
     1081 cgccttctcc gcggcttcct tggccttggc gaccggcttg gcgaccgcct tcttcgcctt
     1141 ggccaccggc ttggcggcgg ccttcttcgc cttggcgacg ggcttcttca ccgcggcctt
     1201 ggccttgctc tggacttttt tcaccgaagc cttggccttg ctggtgctct tcttggcctt
     1261 ggtggtcgcc ttcttggcgg aggccttcac tttcgccagc gccttcttgg cggtggcctt
     1321 gagcttggcg agacggttgg gcttggcagc cttggcggct ggttttctgg atttcttcat
     1381 ggacctgcgt gctcctgctt tggcgtcttg gggcgccgga tctggtgtgg gccgagttcg
     1441 tatctgcgaa gcgaggaagg aaacgtctct tttttcctcc cttgactttc cccctgaaat
     1501 gcgaaatcga gtgtaggcgc ggccccagag gctgtcaaaa acatgtcacc tgcgaggacg
     1561 cgcaggagga atttccattg ttgtttacgt gccgaaggcg cggcggctat gattgccgcc
     1621 ccttgtcggg gtccgatctg ccgaccccaa tcctcgattc ctttttcctt tcctactcaa
     1681 cgaggttcca gccatgatcc aggccatttt cgagggccag tcgctcaagg tcacgctgct
     1741 cgacggcggc attgccgagc tgtgcttcga tcgccagaac gactccatca acaagttcga
     1801 tgcgcgcacc gtcaacgagc tgcgcgaagc cgccgacaag ctgcagaacg ccggcaacgt
     1861 gaagggcctg ctggtgacca gcgcaaagga cgtcttcatc gtcggcgccg acatcaccga
     1921 gttcggccag aacttcaccc gcaccgaaga agaaattgcc gcctggggcg tggaagccaa
     1981 caaggtgttc agcgcggtgg aagacctgcc gttcccgtcg gtcgtcgcca tcaacggctt
     2041 cgcgctgggc ggcggattcg agatgtgcct gtcggtagat taccgcgtga tgtccaccgc
     2101 cgcgcagatc ggctttcccg aggtgaagct gggcatctac ccgggattcg gcggcaccgt
     2161 gcgcgcgcca cgcctgatgg gcgcggacaa cgccatcgag tggatcgccg gcggcgagaa
     2221 ccagaagccg gacaccgcgc tcaagctgca cgcggtcgac gctgtgacgg cgccggacaa
     2281 gctgcgagcc gccgcgctca agctgctggg ccaggccatc gaaggcaagt acgactggaa
     2341 ggcgcgccgc gcgcagaaga ccggcaggct caagctggac cagatcgaag caatgatggt
     2401 gttcacctcg gccaagggtt tcatcgccgg caaggccggc aagaactacc cggcgccgct
     2461 ggcggccgtg tcggccatcg agaaggccgc gggcaagacg cgcgacgacg ctatcaaaat
     2521 agaagcagcg ggttttgcca aggtggccaa gacgccgcag gccgacgcgc tgatcggcct
     2581 gttcctcaac gaccagttcc tgaagaagaa ggccaagggc cagggcaaga acgccaccaa
     2641 gccggtgaag caggcggcgg tgctgggcgc cggcatcatg ggcggcggca tcgcctacca
     2701 gtcggcggtg aagggcacgc cgatcatcat gaaggacatc aaggatgaac agctgcatct
     2761 gggcatgtcc gaggcgaaca agctgctgtc gaagatggtc gagcgcaaga agctgacgcc
     2821 ggagaaggcg ggcgacgtgc tgtcgaacat ccgcgccacg ctcaactacg gcgatttcca
     2881 gggcgtcgac atcgtggtcg aggccgtggt cgagaacccc aaggtgaaga agagcgtgct
     2941 ggccgaggtc gaggggctgg tcaagcccga caccatcatc gcctcgaaca cctcgtcgat
     3001 ttccatcgac gaactgtcca ccgcgatgaa gcgcccggag aatttcctgg gcatgcactt
     3061 cttcaacccg gtgcacaaga tgccgctggt ggaggtgatc tacggcgcca agaccagcaa
     3121 ggcggccatc ggcagtgtgg tggcgtatgc cagcgccatg ggcaagacgc ccatagtcgt
     3181 gaagaactgc ccgggcttcc tggtcaaccg catcctgttc ccgtacttcg gcggcttcat
     3241 gggcgtgctg cgcgatggcg ccgacttcgt caaggtcgac aaggtgatgg agaacttcgg
     3301 ctggcccatg ggcccggcct acctgcagga cgtggtcggc atggacacca gccatcatgt
     3361 cggcgacgtg ctggccgagg gctacccgga ccgcatgggc aaggagttca agagcgcgct
     3421 cgacgtgatg tacgagaaca agcgctacgg ccagaagaac ggcgtgggct tctacaagta
     3481 cgagaacgat cccaagggca aaccgaagaa gatcgccgat cccacctcat atgaactgct
     3541 caagagcgtg cagcccaacg gccagaagga catgagcgac gaggagatca tcgaccgcct
     3601 gatgctgccg atgatcatcg agaccgcgcg ctgcctggac gagggcatcg tcgagagccc
     3661 gcaggaagcc gatatgggcc tgatcctggg catcggcttc ccgccgttcc gcggcggcgc
     3721 gctcaagtac gccgaccagg tcggcatgaa gaccgtgctg gaaaaggccg ccaagtacga
     3781 gaagctcggc aagctctacc acccgaccga ctccatgaag aagatggcgg cggaaggcaa
     3841 gacctactac tcgaagtaat cggagaattc acatgaccga tgtcgtgatt gtcgatgcca
     3901 tccgcacccc gatgggccgc agcaagggcg gcgcgttccg caacgtgcgc gcggaagacc
     3961 tgtcggcgca cctgatccgc accatcctgc agcgcaacgc cgcggtgaag ccggccgacg
     4021 tggaagacgt gatctggggc tgcgtgcagc agaccctgga acagggcttc aacatcgcgc
     4081 gcaacgccgc gctgctggcc ggcctgccgc aaagcgtgtc cggccagacc gtcaaccgcc
     4141 tgtgcggctc gtcgatgacg gcgatccacg ccgcggtcgg cagcatcaag gccggcgtgg
     4201 gcgacacctt catctgcggc ggcgtcgagc acatgggcca cgtgccgatg acgctcaact
     4261 tcgacggcga tcccgcgctg tcgctgtcgg tggccaaggc ctccgccatg atgggcctga
     4321 ccgcggagat gctgacgcag acccaccagg tgggtcgcaa ggagcaggac gagttcgcgc
     4381 tggaatcgca caagcgcgcc tacaacgcgt ggaagaccgg ccagttcaag aacgagatgg
     4441 tgccggtgga aggccacgac caggacggcg cgcccatcct ggtggacttc gacgaggtgg
     4501 tgcgttccga tgccagcatc gagggcctca acagcctgaa gccggtgttc aaccccaagg
     4561 gttcggtcac cgccggcaac agttcggcga tttccgacgg ggcgtcgtgc accctgatca
     4621 tgaacgctga caaggccaag gcgctcggcc tgaagccgct ggcgaagatc gtttccatgg
     4681 cttccgcggg ctgcaacccg tcgatcatgg gcatcggccc ggtgccggca agcaagaagg
     4741 cgctgacccg tgcggggctc gatctcaaga acatcgacta cttcgagctg aacgaagcct
     4801 tcgcagcgca gtcgctggcg gtgatgaagg agttgaagat cctcgaccgc atggaccgcg
     4861 tgaacatcaa gggcggcgcc atcgcgctgg gtcacccact gggctgctcg ggcgcgcgca
     4921 tcaccggcgc gctggcgcac gtgctcaacg agaagaacgc gcagttcggc ctcgccacca
     4981 tgtgcatcgg catgggccag ggtgtggcca cggtgctgga gcgggtgcac tgaggcgatt
     5041 ggcggtcatg aggtaaaaca gaaggccgga gcgatccggc ctttttcttt gcccgcccgg
     5101 acgcggcgaa aggccggatg ttagaatcgc gcgcgctcgc ggcagaggat ttggacgggg
     5161 ttggataggc agcaatgatc tgggtcgact actgcatcct cggcgtgacc ctggtttcca
     5221 tcatcatcgg cgcattccgc ggcgtggtcc gcgaagtgct aggcctgctg acctgggcgg
     5281 tggccttcct cgccgccgcg ctgttccaca acgaggtggc cgggctgctg cagagcaaga
     5341 tctccaaccc ggcgctgtgc acggccgccg gttgggccgg caccttcctt gcggggctgc
     5401 tggtcggcgc catcatctcc agcgtgctgg tcgaggcggt gcgcaacagc accttctccg
     5461 gcgccgaccg caccatcggc gcgggcttcg gcctcttgcg cgggctgctg ctcaccgcaa
     5521 tattcctgct ggtagccggc agcatgggcg agaagaagag cgagtggtgg cagcgctcgc
     5581 tgttcgtatc caagctggaa tggctggctg acgggctggg catggtggtg ccgcagtcct
     5641 ggctggaaaa gctcaatcct gacgcagcgc caaccaaagg aagttgaacc atgtgtggca
     5701 tcgccggcat cgtgacccag ggcagcgccg tcaactccga gctgttcgac gcgctgacca
     5761 tgctgcagca tcgcggccag gatgccgccg gtatcgccac ctcgggggac ggggagctct
     5821 gcgtgcgcaa gggcagcggg ctcgtgcgcg atgtgtttca gcagcatcac atgatcgagc
     5881 tccgcggaaa cctcggcatc ggccacgtgc gctatccgac cgccggct
//
LOCUS       DASZZC010000212         5696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_725230,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000212 DASZZC010000000
VERSION     DASZZC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725230"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VHE37_04615"
     CDS             <1..857
                     /locus_tag="VHE37_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HVT34837.1"
                     /translation="KPGDSTNIDVQERLFMRERMAKLEIAPLSSMYFFGENSPAHLED
                     YRPEVHDSEGLQVQTDHEWIWRPLVNPKRVLITAFGAINPRGFGLMQRDRNFAHYEDL
                     EARYDLRPSCWITPKNDWGPGRVELTLLPTPDETNDNVVAYWVPDNLPAPGQPIDLQY
                     TLSWQMKNETRPTMARVVQTRRGHGWWKDDDGSMRFNVDFEGGDIAKLAAANQIVAGV
                     WVGENGELMERQSYRNDVTGGWRVSLRIRREEEDRPVEMRVVLRDPKGAISETWAYIL
                     PARTAPTD"
     gene            971..3478
                     /gene="mdoH"
                     /locus_tag="VHE37_04620"
     CDS             971..3478
                     /gene="mdoH"
                     /locus_tag="VHE37_04620"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HVT34838.1"
                     /translation="MDPKIALRARLESYLQHLPLTETRRSALRVRLEPAIARNDTALL
                     FAELHRALADAAQADRENPPRQSLPARLDMALADEHKRGDEHAGESFTTDVQGQARLV
                     TTPPLARASMRPTPWANNPFKRLAHWLRDRTIGRRGMEPHAPAAAADDDAPTSARRRP
                     VRWRTAGRNRRFLLLALILLQTYLATYFMTAVLPYHGAHILEKAILVIYVILFSWVGA
                     GFWTAMMGFWVLLLGRDKYSINASAAPDLPIDAATRTALLIPICNEDVARVYAGLRAT
                     YDSVMRAGGRQHFDFYILSDTNNADTRVAELEAWLQLCREVDGFGHIHYRRRRHRIKR
                     KSGNIADWCRRWGSRYQYMVILDADSVMSGACLKRLVQLMEANPTAGIIQTAPRAAGR
                     ETLYSRIQQFATRAYGPLFTAGLHYWQLGESHYWGHNAIIRVAPFMRHCALGRLPGRG
                     ALSGEIMSHDFVEAALMRRAGWAVWIAYDLPGSYEEMPPTLLDELRRDQRWCQGNLQN
                     FRLFMTRGLHPAHRAVFMTGVMAYISAPLWFAFLALSTALLWVQTKTEPVYFTQPYQL
                     FPTWPEWHPEWAKHLFAATAGLLFLPKILATLLLLAQRAQLRLFGGPAKLVITLLLEM
                     IFSALLAPVRMLFHTRYVTFALAGIAVAWKSPPREDAQTPWSEALRRHAGGTVLGMAW
                     LGFVYWLQPTFLWWLLPVAGAMVIAIPLSVWSSYIGLGRWSRDARLFLIPEEAQPPRE
                     LRWTRAGVRRARDWSGGFVRAVHDPITNALVCAAAHPRERLPESDKQKRRELVSSALD
                     TGLGKLSDVQRNALLSDRDGLALMHHKVWTAREFDAG"
     gene            complement(3489..4865)
                     /locus_tag="VHE37_04625"
     CDS             complement(3489..4865)
                     /locus_tag="VHE37_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVT34839.1"
                     /translation="MEKNRDADTDAAQGAPRRRRVRLPLPQIPRISWRDLVATLGPVL
                     LLAAAAVFAALHFVRPAPPRTLTISSGPPGSSYASMAEQYRDILADSGITLKIVASEG
                     SLANLHSLIDEDSDVDVAFVPSGISAGEDISELVSLGSVFYQPLTIFYRREQPLERLS
                     QLAGGRIAIGAVGSGTRIIALDLLKANGIEPGGTTQLEPIEGGAAIDALMARKVDAIF
                     LSGDSASRQNIRQLLHEKGVRLFDFPQAEAYARRFRYLTRLEIPAGAFDLGENLPAKN
                     ISMLAPTVELVARWDLHPALSDLLIETARQVNGKASVLQEAGEFPAPRPHDFPLSDDA
                     QRYYKSGKGYAYRFLPFWLASLVDRAVVVLVPLIVVLLPGLRVVPALYAWRVKNRIYK
                     HYGALMAVERAALAPMTPEQREDLRARLAEIEKAVIEVKMPGAYANQLYVLREHIKFV
                     QDQLVSGR"
     gene            4946..5677
                     /locus_tag="VHE37_04630"
     CDS             4946..5677
                     /locus_tag="VHE37_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT34840.1"
                     /translation="MSDNRTAVVIGVGALRGLGAAVARRFAREGFVAVVAGRTQAQLD
                     AVAQAIRDAGGQAHALVADAASEEQVTQLLERAEAIAPIGVAVYNAGGNSPRASLDTG
                     GEFFEKMWRVGTYGGLNFAREAVRRMLPRAQGTLLFTGASASLRGRPDFVAFAAAKAG
                     LRAVSQSFAREFGPKGIHVAHVVIDGAIDGERIHSIVPQLAKMKGEAGLLDLEAIAEA
                     YWQLHRQHRTAWTQELDLRPYKEPF"
BASE COUNT          894 a         2002 c         1932 g          868 t
ORIGIN      
        1 tcaagcccgg cgactccacc aacatcgacg tgcaggagcg cctgttcatg cgcgagcgca
       61 tggccaagct ggagatcgcg ccgctgtcgt cgatgtattt cttcggcgag aacagccccg
      121 cacacctgga agactaccgg cccgaggtgc acgactccga gggcctgcag gtgcagaccg
      181 accacgagtg gatctggcgg ccgctggtca atcccaagcg cgtgctgatc accgcattcg
      241 gcgcgatcaa cccgcgcggc ttcggcctga tgcagcgcga ccgcaacttc gcccactacg
      301 aagacctcga ggcgcgctac gacctgcgcc cgtcgtgctg gatcacgccg aagaacgact
      361 ggggcccggg ccgcgtggag ctgaccctgc tgcccacgcc tgatgagacc aacgacaacg
      421 tggtcgccta ctgggtgccg gacaacctgc cggcgccggg ccagccgatc gacctgcagt
      481 acacgctgtc gtggcagatg aagaacgaga cgcggccgac catggcgcgg gtggtgcaaa
      541 cgcggcgcgg ccacggctgg tggaaggacg acgacggcag catgcgcttc aacgtcgact
      601 tcgagggcgg cgacatcgcc aagctggctg cggcgaacca gatcgtcgcc ggcgtctggg
      661 tcggcgagaa cggcgagctg atggaacgcc agagctaccg caacgacgtc accggcggct
      721 ggcgcgtgtc gctgcgcatc aggcgcgagg aagaagaccg cccggtggaa atgcgcgtgg
      781 tgctgcgcga ccccaaggga gcaatttccg agacctgggc ttatattctg ccggcccgca
      841 ccgcgcccac cgactgagcc gccgcggaga gccagaaaaa caccccaccg cactgccgcc
      901 tgcgctgtta ttttttattg atcgattgta aaatatgtta gcaattgata acagctccaa
      961 ggccggtgcg gtggacccca aaattgccct gcgcgcgcgg ctggagtcct acctccagca
     1021 cctgccgctg accgagacgc ggcgcagcgc cctgcgcgtg cggctcgaac cggccatcgc
     1081 gcgcaacgac accgcgctgc tgttcgccga gttgcaccgc gcgctggccg atgccgcgca
     1141 agccgaccgc gagaatccgc cgcggcagtc gctgcctgcg cggctggaca tggcgctggc
     1201 cgacgagcac aagcgcggcg acgagcacgc cggcgaaagt ttcaccaccg acgtgcaggg
     1261 ccaggcgcgc ctggtcacca ccccgccgct ggcgcgcgcc tccatgcggc ccacgccgtg
     1321 ggccaacaat ccgttcaagc gcttggccca ctggctgcgc gacaggacca tcggccggcg
     1381 cggcatggag ccgcacgcac ccgctgccgc ggccgacgac gacgcaccca ccagcgcgcg
     1441 gcgccgtccg gtgcgctggc gcacggccgg gcgcaaccgc cgcttcctgc tgctggcgct
     1501 gatcctgctg cagacctatc tcgccaccta cttcatgacc gcggtgctgc cgtatcacgg
     1561 cgcgcacatc ctggaaaaag cgatcctggt gatctacgtg atcctgttct cctgggtcgg
     1621 cgccggcttc tggaccgcga tgatgggttt ctgggtgctg ctgctcgggc gcgacaagta
     1681 ctcgatcaac gccagcgccg cgccggacct gccgatcgat gcggccacgc gcaccgcgct
     1741 gctgatcccg atctgcaacg aggacgtggc gcgcgtgtac gccggcctgc gcgccaccta
     1801 cgactcggtg atgcgcgcgg gcggacgcca gcatttcgat ttctacatcc tgtccgacac
     1861 caacaacgcc gatacgcgcg tggccgagct ggaagcctgg ctgcagctgt gccgcgaggt
     1921 ggacggcttc ggccacatcc attaccgccg ccgccgtcac cggataaaaa gaaagagcgg
     1981 caacatcgcc gactggtgcc gccgctgggg cagcaggtat caatacatgg tgatcctcga
     2041 cgccgacagc gtgatgtccg gcgcctgcct caagcggctg gtgcagctga tggaagccaa
     2101 cccgaccgcc ggcatcatcc agaccgcgcc gcgcgccgcc ggccgcgaga cgctgtactc
     2161 gcgcatccag cagttcgcca cccgcgcgta cggaccgctg ttcaccgccg gcctgcatta
     2221 ctggcagctc ggcgaatcgc actactgggg ccacaacgcc atcatccggg tggcgccgtt
     2281 catgcgccat tgcgcgctgg gccgcctgcc cgggcgcggc gcgctgtcgg gcgagatcat
     2341 gtcgcacgac ttcgtcgagg ccgcactgat gcgccgcgcc ggctgggcgg tgtggatcgc
     2401 ctacgacctg ccgggctcgt acgaggaaat gccgccgacg ctgctcgacg agctgcgccg
     2461 cgaccagcgc tggtgccagg gcaacctgca gaacttccgc ctgttcatga cgcgcggcct
     2521 gcaccccgcg caccgcgcgg tgttcatgac cggcgtgatg gcctacatca gcgcgcccct
     2581 gtggttcgcc tttctggcgc tgtccaccgc gctgctgtgg gtgcagacca agaccgagcc
     2641 ggtgtacttc acccagccgt accagttgtt tcccacctgg ccggaatggc acccggaatg
     2701 ggccaagcac ctgttcgccg ccaccgccgg cctgctgttc ctgccgaaaa tcctcgcaac
     2761 gctcttgtta ttggcgcagc gcgcgcagct caggctgttc ggcgggccgg ccaagctggt
     2821 catcacgctg ctgctggaaa tgatcttctc ggcgctgctg gcgccggtgc gcatgctgtt
     2881 ccacacgcgc tacgtcacct ttgcgctggc cggcatcgcg gtggcgtgga aatcgccgcc
     2941 gcgcgaggac gcgcagacgc cgtggagcga ggcgctcagg cgccatgccg gcggcacggt
     3001 gctgggcatg gcctggctgg gcttcgtcta ctggctgcag cccaccttcc tgtggtggct
     3061 gctgccggtg gccggcgcca tggtaatcgc catcccgctg tcggtgtgga gttcgtatat
     3121 cgggctgggc cgctggagcc gcgacgcgcg cctgttcctg atcccggaag aagcgcagcc
     3181 gccgcgcgaa ctgcgctgga cccgcgccgg cgtgcgccgc gcgcgcgact ggtcgggcgg
     3241 cttcgtccgc gcggtgcacg accccatcac caacgcgctg gtgtgcgcgg ccgcgcatcc
     3301 gcgcgagcgc ctgccggaga gcgacaagca gaagcggcgc gagctggtgt ccagcgcgct
     3361 ggacaccggc ctgggcaagc tgtccgacgt gcagcgcaat gcgctgctgt ccgaccgcga
     3421 cggcctggcg ctgatgcacc acaaggtatg gacggcgcgc gagttcgacg ccggctgaag
     3481 cggtcgcgtc aacgaccgct gacgagctgg tcctgcacga acttgatgtg ctcgcgcagc
     3541 acgtagagct ggttggcgta ggcgccgggc atcttcacct cgatcaccgc cttctcgatt
     3601 tccgccagcc gcgcgcgcag atcctcgcgc tgctccggcg tcatcggcgc cagcgccgcg
     3661 cgctccaccg ccatcagcgc gccatagtgc ttgtagatgc ggttcttcac ccgccacgcg
     3721 tacagcgccg gcaccacgcg caggcccggc agcagcacca cgatcagcgg caccagcacc
     3781 accaccgcgc ggtcgaccag gctggccagc cagaacggca ggaagcggta ggcgtagccc
     3841 ttgcccgact tgtagtagcg ctgcgcgtcg tcgctgagcg gaaaatcatg cggccgcggc
     3901 gccgggaatt ctccggcttc ctgcagcacg ctggccttgc cgttgacctg ccgcgcggtt
     3961 tcgatcagca ggtccgacag cgccgggtgc aggtcccagc gcgccaccag ctccaccgtc
     4021 ggcgccagca tggagatgtt tttcgccggc aggttctcgc ccaggtcgaa cgcgccggcc
     4081 ggaatttcca gccgcgtcag gtagcggaag cgccgcgcgt aggcctcggc ctgcggaaag
     4141 tcgaacaggc gcacgccctt ctcgtgcagc agctggcgga tgttctggcg gctggccgaa
     4201 tcgccggaca ggaaaatcgc gtcgaccttg cgcgccatca gcgcgtcgat ggcagcgccg
     4261 ccttcgatcg gctccagctg tgtcgtgccg cccggctcga tgccgttggc cttgagcagg
     4321 tcgagcgcaa tgatgcgcgt gccgctgccc acggcgccga tggcgatgcg cccgccggcc
     4381 agttgcgaca gccgttccag cggctgctcg cgccggtaga agatggtcag cggctggtag
     4441 aacacgctgc ccagcgagac cagctcgctg atgtcctcgc cagcggaaat tccgctcggc
     4501 acgaaggcga cgtcgacgtc ggaatcctcg tcgatcaggc tgtgcagatt ggccagcgag
     4561 ccctccgagg cgacgatctt cagggtgatg ccgctgtcgg ccaggatgtc gcggtactgc
     4621 tcggccatgc tggcgtagct gctgcccggc ggcccgctgc tgatggtcag cgtgcgcggc
     4681 ggcgccggcc gcacgaaatg cagcgcggcg aacaccgcgg ccgcggcgag cagcagcacc
     4741 gggccgagcg tggcaaccag gtcgcgccag gagatgcgcg gaatctgtgg caacggcagc
     4801 cgcacgcggc ggcggcgcgg cgcgccttgc gcagcgtcag tatcggcatc ccgatttttt
     4861 tccatggccg gattgtcctt tatttgcgca gcgcgctggc ggcgcggagc gtatctttac
     4921 gcgggcctcg gcagcggagc acgtcatgtc agacaaccgg acagcggtgg tgatcggcgt
     4981 cggcgcgttg cgcggactgg gtgcggctgt agcacggcgt ttcgcgcgcg aaggcttcgt
     5041 cgccgtcgtt gccgggcgca cgcaggcgca gctcgatgcg gtagcgcagg ccatccgcga
     5101 cgccggcggc caggcgcacg ccctggtcgc cgatgccgcc tcggaagaac aggtgacgca
     5161 gctgctcgag cgcgcggaag ccatcgcgcc catcggcgtg gcggtataca acgctggcgg
     5221 caacagcccg cgcgccagtc tcgacaccgg cggcgaattc ttcgagaaga tgtggcgcgt
     5281 cggcacctac ggtggcctga acttcgcgcg cgaggcggtg cggcgcatgc tgccgcgcgc
     5341 gcagggcacg ctgctgttca ccggcgcctc ggcctcgctg cgcggccggc cggacttcgt
     5401 cgccttcgcc gcggccaagg ccggcctgcg cgcggtgagc cagtcgttcg cgcgcgagtt
     5461 cggccccaag ggcatccacg tggcgcacgt ggtgatcgac ggcgccatcg acggcgagcg
     5521 catccacagc atcgtgccgc agctggcgaa gatgaagggc gaggccgggc tgctcgatct
     5581 cgaggccatc gccgaggcct actggcagct gcaccggcag caccgcacgg cgtggaccca
     5641 ggagctcgac ctgcggccgt acaaggagcc gttctagcgc cattaccagt ctggac
//
LOCUS       DASZZC010000213         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_531030,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000213 DASZZC010000000
VERSION     DASZZC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531030"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..530
                     /locus_tag="VHE37_04635"
     CDS             372..530
                     /locus_tag="VHE37_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HVT34841.1"
                     /translation="MTLGTVALIVLLLLLVGAIPSWPYSRNWGYAPSGSLGLVLIIVL
                     VLLATGRI"
     gene            532..714
                     /locus_tag="VHE37_04640"
     CDS             532..714
                     /locus_tag="VHE37_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34842.1"
                     /translation="MSFALYLLGFAIAIAGACYGAWLLHVPQQWIAVGAIVLLGLAIM
                     AGVRSTRQRDPSPPTR"
     gene            765..>1858
                     /locus_tag="VHE37_04645"
     CDS             765..>1858
                     /locus_tag="VHE37_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HVT34843.1"
                     /translation="MSAAAAGVLGDTPPAPPAATAPAPVAFAGNPPTPKLDWGAGDGR
                     SFAIPALEIIGFEFTLNQFDRHYAHPREDYISNIHTIRDNLQRRWVVDNDPFSVNQFG
                     HPYQGSLYHGFARSSGLSYWEALAYDHAGSVLWEIAGERTAPSINDQINTGFGGSFLG
                     EPLFRMASLTLETASGKPGFWRELGATFLSPAAGFNRAVSDRFDGVFPSHDPAVYTRV
                     QAGTALTDTASGRVSGVTSFDRNQAYAEVLVEYGLPGKDGYTYDRPFDYFNFQFTAST
                     SNTFESILSRGLLVGQAYHDGNNYRGLWGLFGTYDYIAPQVFRVGSTGASVGTVLQWW
                     ASNNVAVEATVMAGLGYAAGGVTEQRDGFR"
BASE COUNT          312 a          630 c          584 g          332 t
ORIGIN      
        1 gcgcagcgaa gatccgaaga agatcttcga ggagttctac ggcggcccgg cggacgtcgg
       61 cccgctgctc gagcatcgcg ggctcaagct gcccgcgaag aaagccgaag caaagaagta
      121 gcgcggcgct tcgcggtgaa cccaggcccc gccggcgacg ccggcggggc tttttcatgc
      181 gcgaaatagg cctgaccgca tcgatatttt tgatgttatt aaatatttaa taaagataat
      241 aatagattat atttaataac agcgcccggg cgggtgcggt ggaggaattt ttcggcacac
      301 ggtcatcgtt gccgaactcc gccgctgcga aaacagtcct tcagccagga aggaaaatcg
      361 cggagcccgt catgacgcta ggaaccgttg cactcatcgt cctgttgctg ttgctggtcg
      421 gcgccattcc cagttggccg tacagccgca actggggtta tgcgcccagc ggctcgctcg
      481 ggctggtgct gatcatcgtg ctggtgctgc tggccaccgg gcgcatctga cgtgtcgttc
      541 gcgctttacc tgctcggctt tgccattgcc atcgccggcg cgtgctacgg cgcgtggctg
      601 ctgcacgtgc cgcagcagtg gatcgcggtc ggcgccatcg tgctgctcgg gctggccatc
      661 atggccggcg tgcgcagcac gcggcagcgc gatcccagcc cgccgacgcg ttgaacgcca
      721 tccggcccgt cgcgtccgcg agtgccgccg cgctcgcatt ttttttgtcc gccgccgcgg
      781 ccggcgtgct tggcgacacg ccgccggcac cgccagcggc cacggcgccc gcgccggtcg
      841 ccttcgctgg caacccaccg acacccaagc tcgactgggg cgcgggcgat ggccgcagct
      901 tcgccattcc ggcgctggaa atcatcggct tcgagttcac cctcaaccag ttcgaccgcc
      961 attacgcgca cccgcgcgag gattacatct ccaacatcca caccatccgc gacaacctgc
     1021 aacgccgctg ggtggtggac aacgatccgt tttcggtgaa ccagttcggc catccatacc
     1081 agggcagcct ctaccatggc ttcgcccgct cgtccggcct cagttactgg gaagcgctgg
     1141 cctacgacca cgcgggcagc gtgctgtggg aaattgccgg cgagcgcacc gcgccgtcga
     1201 tcaacgacca gatcaatacc ggcttcggcg gcagtttcct cggcgagccg ctgttccgca
     1261 tggccagcct cacgctggaa acggccagcg gcaaacccgg cttctggcgc gaactcggcg
     1321 ccaccttcct gtcgccggcc gccggcttca accgcgccgt gtctgaccgc ttcgacggcg
     1381 tgttccccag ccatgacccg gcggtgtata cgcgggtgca ggcgggcacc gcactcaccg
     1441 acaccgccag cgggcgcgtg tcgggcgtga ccagcttcga ccgcaaccag gcttacgccg
     1501 aggtgctggt ggaatacgga ctgccgggca aggacggcta cacctacgat cggccgttcg
     1561 actatttcaa cttccagttc accgccagca cttcgaacac cttcgagagc atcctgtcgc
     1621 gcggcctgct ggtggggcag gcgtaccacg acggaaataa ttaccgcggg ctgtggggcc
     1681 tgtttggcac ctacgactac atcgcaccgc aggtattccg cgtcggcagc accggcgcgt
     1741 cggtgggcac cgtgctgcag tggtgggcat cgaacaatgt tgccgtcgag gccaccgtga
     1801 tggccggcct gggctatgcc gccggcggcg tcaccgagca gcgcgacggc ttccgcga
//
LOCUS       DASZZC010000214         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_467923,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000214 DASZZC010000000
VERSION     DASZZC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467923"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VHE37_04650"
     CDS             complement(<1..762)
                     /locus_tag="VHE37_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34844.1"
                     /translation="MAQIFRVVLPVKDLQQAVQFYRGLLGVDGERISPGWHYFTLGLC
                     VLACHDANAEGDVPGVPHSSPVCIAVDEPLPLLRVRAGKLGPRSIDEQVGLLPTGESG
                     FTLQDPSGNALCLIDSRTVRRGRPQAARPRTDDSFVLQFRRDFLNAVKGGELARVREL
                     LALDPALIQATDEAGVSALMLAGYKRHERVAAFLLSQRQELTAWEAASFGLPERLREC
                     LGLRPELVGEVSTDGFTLLGLACFFGQFECVALLIE"
     gene            945..1838
                     /locus_tag="VHE37_04655"
     CDS             945..1838
                     /locus_tag="VHE37_04655"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HVT34845.1"
                     /translation="MKQKLAATLVALASLLVSLTLHAQDDGSSNNPGAAVLASLKKQT
                     GEITLPNGIATLKLTDDFYFLDQANARKMLTEGWGNPPQAADRVLGMVLPAHANPLSR
                     QGWGVVITYDEDGHVSDSDADTIDYAQMLKDMKQQAEDSNSARKQAGYPEVHLSGWAE
                     NPHYDPAAHKIYWAKDLQFADGPDHTLNYFVRVLGRKGVLELNAIAGMSQLPAVREDM
                     KQVLALTEFTPGNRYNDFNSSTDKVAAYGLAALVAGGIAAKTGLLAKLGVLLLSLKKF
                     VILIFAAIAGFFKKIFGGKKQ"
     gene            complement(1839..2396)
                     /locus_tag="VHE37_04660"
     CDS             complement(1839..2396)
                     /locus_tag="VHE37_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HVT34846.1"
                     /translation="MTGNEPTDCLYQRALGPAWARLPAPLRQLHEVRGRHAWQGMASV
                     ENGRSLLARLCRVLMGFPAAAAEVPVRVTLERIGESERWTREFGASRFHSVLSLPRDG
                     EAGVVIERFGLLSFRLGLPADEHGLEMPMRGARVLGLPLPRLLTPRSRTREFVDDQGR
                     FCFDVAVSLPAAGLLVHYRGWLLPS"
     gene            2510..>3543
                     /locus_tag="VHE37_04665"
     CDS             2510..>3543
                     /locus_tag="VHE37_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVT34847.1"
                     /translation="MGESAGQDRATLASFYGDMERLLTTQLQASPKDHNVRLKLLEVY
                     YETHRKSDFLREAHNLARDFDRKRRAADWNRMVSMGRMLGLEDRLFDEHVGDSITFVE
                     PSKLGPGNAPGHHQRIGESPEYQEYFARLADAWAQMQADPRFLIDLDQELLRSANRPS
                     SMQNAKRLTVSRKGAQIYFKREDLSPPGSQLSIAIIGQALLAQKLYKNTLVTATKTGF
                     RGVVTAAAAARLGLKCRVFMDREDMHRHQQTVFQMRLLGAEMIGVTSTRFQDNDVRIG
                     ALEHCAENPDKNFLALGLDGAPPPYPTMTREFLSTIGRESRRQVNAFSKRPPDLLAAR
                     GGDTLDAIGF"
BASE COUNT          659 a         1232 c         1131 g          521 t
ORIGIN      
        1 ctcgatcagc agcgccacgc attcgaactg gccgaagaag caggccaggc ccagcagcgt
       61 gaaaccgtcg gtcgatactt ctccgaccag ctcggggcgc aggcccaggc attcgcgcag
      121 gcgttccggc aacccgaacg atgccgcttc ccaggccgtg agttcctggc gctgcgacag
      181 caggaaagcg gccacacgct cgtggcgttt gtagccggcc agcatcagcg cgctgacgcc
      241 cgcctcgtcg gtggcctgga tcagcgccgg gtccagcgcc agcagttcgc gcacgcgcgc
      301 cagttcgccg cctttcactg cgttgaggaa atcgcgccgg aactgcagca cgaagctgtc
      361 atcggtgcgc ggccgcgccg cctgcggccg tccgcggcgt accgtgcggc tgtcgatcag
      421 gcacaacgcg ttgccgctcg gatcctgcag ggtgaagccg ctctcgccgg tcggcagcag
      481 gccgacctgc tcatcgatgg aacgcgggcc cagcttgccc gcgcgcacgc gcagcagcgg
      541 cagcggctcg tccaccgcga tgcacaccgg cgaggaatgc ggcacgcccg gcacgtcgcc
      601 ttccgcgttg gcgtcgtggc aggccagcac gcacaggccc agcgtgaagt agtgccagcc
      661 cgggctgatg cgctctccat ccacgcccag caatccacgg taaaactgca ccgcctgctg
      721 caggtccttg accggcagca ccacacggaa aatctgcgcc atctctcgcc atgccggatg
      781 tagcgggcgc acacggcgcc gcgaatcacc cgcccccttt cttgtcgtcg gcggattcta
      841 gtcggccatg cgcagcgcgg cctgagtgtt ttcctggggg caaaacggac ggcccgccac
      901 ctagaatgaa ccaggctgcg gggacagccc cggggagaaa aaacatgaaa caaaaactgg
      961 ccgcaacctt ggttgcgctc gcatcattgc tggtctcgct gacgctgcat gcgcaggatg
     1021 atggcagcag caacaatccc ggcgcggccg tcctcgccag cctgaagaag cagaccggcg
     1081 agatcacgct gcccaatggc atcgccacgc tcaagctgac cgacgacttc tactttctcg
     1141 accaggccaa cgcccgcaag atgctcaccg agggctgggg caatccgccg caggcagccg
     1201 accgcgtact gggcatggtg ttgccggccc atgccaatcc gctgagcagg caaggctggg
     1261 gcgtggtcat cacctacgac gaggatggcc acgtgtcgga cagcgacgcc gacaccatcg
     1321 actacgcgca gatgctcaag gacatgaagc agcaggcgga ggattccaac agcgcgcgca
     1381 agcaggccgg ctacccggaa gtgcacctgt ccggctgggc ggaaaacccg cactacgatc
     1441 cggccgccca caagatctac tgggccaagg acctgcagtt cgccgacggg cccgatcaca
     1501 cgctgaacta cttcgtgcgc gtgctcggcc gcaagggcgt gctggaactc aacgccatcg
     1561 ccggcatgtc gcaactgccg gcggtgcgcg aggacatgaa gcaggtgctg gccctgaccg
     1621 agttcacccc gggcaaccgc tacaacgatt tcaattcgtc gaccgacaag gtcgcggcgt
     1681 atgggctggc cgcgctggtc gccggcggca tcgccgccaa gaccggcctg ctggccaagc
     1741 tgggtgtgct gctgctgtcg ctgaagaaat tcgtgatcct gatcttcgcg gccatcgccg
     1801 gcttcttcaa gaaaatcttc ggcggcaaga agcaatagct aggacggcag cagccagccg
     1861 cggtagtgca ccagcagtcc tgccgccggc aggctgacgg ccacgtcgaa gcagaagcgg
     1921 ccctggtcat caacgaactc gcgcgtgcgg ctgcgcggcg tgagcaggcg cggcaggggc
     1981 aggccgagca cgcgggctcc gcgcatcggc atctccagtc catgctcgtc cgcaggcagg
     2041 cccagccgga agctgagcag gccgaagcgt tcgatcacca cacccgcctc gccgtcacgc
     2101 ggcaggctca gcacggaatg aaagcggctg gcgccgaatt cgcgcgtcca gcgctcgctt
     2161 tcgccgatgc gttcaagcgt gacgcgcacc ggcacttcgg cagctgccgc tgggaaaccc
     2221 atgagcaccc ggcacaggcg ggcgagcagg ctgcggccat tttcgacgct cgccattccc
     2281 tgccaggcat ggcggccgcg gacctcgtgc agctggcgca gcggcgccgg caggcgcgcc
     2341 caggccgggc ccagcgcacg ctgatagaga cagtcggtcg gctcgttgcc ggtcatggaa
     2401 accgtaggca aaaaataccc ggcgagtgta gcggctgcgg catcgcacaa cgggtttttc
     2461 ctacagcgtg cttgcctata ctcgaagcgg gtttgcacgg gaatgggtga tgggggaatc
     2521 agccggacag gatcgcgcga cgctcgccag tttttacggc gacatggagc ggctgctcac
     2581 cacgcagctg caggccagcc cgaaggacca caacgtgcgg ctcaagctgc tcgaggtgta
     2641 ttacgagacg caccgcaaat cggatttcct gcgcgaggcg cacaacctcg cgcgcgactt
     2701 cgaccgcaag cgccgcgccg ccgactggaa ccgcatggtc agcatgggcc gcatgctcgg
     2761 cctggaagac cgcctgttcg acgagcacgt cggtgattcc atcaccttcg tcgaaccctc
     2821 caagctcggt cccggtaatg cgccgggcca tcaccagcgc atcggcgaat cgccggaata
     2881 ccaggagtac ttcgcgcggc tggcggatgc ctgggcgcag atgcaggccg acccgcgctt
     2941 cctgatcgat ctcgaccagg aactgctgcg cagcgccaac cggccctcgt cgatgcagaa
     3001 cgccaagcgc ctgaccgtct ccaggaaagg cgcgcagatc tacttcaagc gcgaagacct
     3061 gtcgccgccg ggctcgcagc tgtccatcgc catcatcggc caggcgctgc tggcgcagaa
     3121 gctgtacaag aacaccctgg tcaccgccac caagaccggt ttccgcggcg tggtcaccgc
     3181 cgccgccgcc gcacgcctgg gcctcaagtg ccgcgtgttc atggaccgcg aggacatgca
     3241 ccggcaccag cagacggtgt tccagatgcg cctgctcggc gccgagatga tcggcgtgac
     3301 cagcacgcgt ttccaggaca acgatgtgcg catcggcgca ctcgagcatt gcgcggaaaa
     3361 cccggacaag aatttcctgg cactggggct cgacggcgcg ccgccgccgt acccgaccat
     3421 gacgcgcgaa ttcctctcca ccatcggccg cgaatcgcgc cgccaggtca acgcgttctc
     3481 caagcgtccg cccgacctgc tggccgcgcg cggcggcgac acgcttgatg cgatcggctt
     3541 ctt
//
LOCUS       DASZZC010000215         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_336879,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000215 DASZZC010000000
VERSION     DASZZC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336879"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VHE37_04670"
     CDS             <1..306
                     /locus_tag="VHE37_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34848.1"
                     /translation="RQPHLVEIGMTPLRIVGATIFLDGLGLIMMQALLGAGAARLVMI
                     VATGLQWGLFLPVAWLVGPAWGLGLTAIWLAMGSYRLLQTVIFTAAWQKRDWVHIRL"
     gene            388..972
                     /locus_tag="VHE37_04675"
     CDS             388..972
                     /locus_tag="VHE37_04675"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HVT34849.1"
                     /translation="MKNTSTIFVAATLGLGLALGGCHKDEQNAADTATATPTASTAMA
                     QQAPTPAPVAPAPAPARTAAAERPHHTTRHQDVASFEAPSCTDCGTITNIEALTQSGT
                     GNTMGTIAGAVIGGVIGHQFGSGRGNTVATAAGAAGGAYAGNRAQAYYNRSTSYKVTV
                     AMQNGGTRTVTVTTLNGMAVGNKVKVVGDTLQPS"
     gene            complement(1023..2249)
                     /locus_tag="VHE37_04680"
     CDS             complement(1023..2249)
                     /locus_tag="VHE37_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT34850.1"
                     /translation="MSGGPFAALRYREFRFLLAASFINTIALLIQEVALSYDLYKLTH
                     DPLVLGLLGLAEAIPFMALALFGGHLADRREKRGLMLASLSVILCGSVVLWWATQARL
                     AQDTLVWTCYAVIGLLGFARGVFSPATASLKPFLVPREHYGNSATWSSTSWQAGAIAG
                     PLLAGLLYAGLGIHHTLLVVIGLILLNLALLGGIARRPAPPAPEAHDNIWASLAEGIA
                     YVRQNKVILYAISLDMFSVLFGGVVAILPVYAQDILAVGAQGLGLLRAAPAVGAMITV
                     IACAWWPPLTHAWRNLLLVVAGFGAATLVFGVSTNIWLSMAALFLTGAFDSVSVIIRS
                     TLLQALPPDHLRGRVVAVNSIFISASNELGAFESGTAARLMGVVPSVLAGAAVTLVTV
                     GWVWRKSTDLLRQKLL"
     gene            complement(2246..3850)
                     /locus_tag="VHE37_04685"
     CDS             complement(2246..3850)
                     /locus_tag="VHE37_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HVT34851.1"
                     /translation="MSDAAQQAALRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTV
                     KGRKAARHATSDWMRLEQQRGISVTSSVMQFPYRDCIVNLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDAAKGVETRTIKLMEVCRLRDTPIITFINKLDREGRPPLELLDEVEKVL
                     GLQCTPITWPLGMGREFKGVYHLLEDRFYLYSGDKHRVSEIETIDGLLSPGLGDRFGA
                     VYQTLRDEIELLQMAGTGFDLDKYRAAKLTPVFFGSGINNFGVRELLNGFTEWAPPPQ
                     ARIAQFKGQDYPIDPAHGKFTGFVFKIQANMDPKHRDRIAFLRICSGTYRKGMQLHSV
                     RLGGHVRVSHALTFMAAEREAVEQAWPGDIIGLHNYGTIAIGDSFTEGEELRFTGIPS
                     FAPELFRRVVLKDPLKAKQLNKGLDQLCEEGATQVYRPLTNNDIVLGAVGALQFEVVQ
                     YRLKDEYGVDSVFDNVAVHNARWVYCDDAKKLKDFVEKNEQRIARDHYGDLVYLASSS
                     VNLSMAQERFPEVKFLDTKAHAVAQT"
     gene            complement(3888..>4174)
                     /locus_tag="VHE37_04690"
     CDS             complement(3888..>4174)
                     /locus_tag="VHE37_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34852.1"
                     /translation="HRIGPATVPQEKPAQPTYLIVNRNRQDDVKFLEVNAVTARLMNL
                     LEEDAASSGRQLLLRIAAELQHPQPEWVVDEGAKILADLRARDIILGTRR"
BASE COUNT          710 a         1408 c         1381 g          675 t
ORIGIN      
        1 cgccagccgc acctggtgga aatcggcatg acgccgctgc gcatcgtcgg cgccacgatc
       61 ttcctcgacg ggctgggcct gatcatgatg caggcgctgc tcggcgccgg cgccgcgcgg
      121 ctggtgatga tcgtcgccac cggcctgcaa tggggcctgt tcctgccggt ggcctggctg
      181 gtcggcccgg cgtggggcct gggccttact gccatctggc tggcgatggg cagctatcgc
      241 ctgctgcaga cggtgatctt cacggcggcg tggcagaagc gtgactgggt gcacatccgc
      301 ctctgattca tgcgcaattc aggctgaacc aattactttg gccgcgggtc tttatccaac
      361 gggagattct tcctcaggag agttcgcatg aaaaacacaa gcaccatttt cgttgccgcc
      421 acgctgggcc tgggcctcgc actgggcggt tgccacaagg acgagcagaa cgcggccgat
      481 accgccacgg ccacgccgac cgcttcgact gccatggccc agcaggctcc cacgccagcg
      541 ccggtggcgc cggcacctgc accggcgcgc acggctgcag ctgagcgtcc gcatcacacg
      601 acgcgtcacc aggatgtggc cagcttcgag gcgcccagct gcaccgactg cggcaccatc
      661 accaacatcg aggcgctgac gcagagcggc accggcaaca ccatgggcac catcgccggc
      721 gcggtgattg gcggcgtcat cggccaccag ttcggcagcg gccgcggcaa caccgtggcg
      781 acggcggccg gcgcggcggg cggcgcctac gcgggcaacc gtgcgcaggc ctattacaac
      841 cgcagcacca gctacaaggt caccgtggcc atgcagaacg gcggcacccg caccgtcacc
      901 gtcaccaccc tcaacgggat ggcggtgggc aacaaggtga aggtggtggg cgacacgctg
      961 cagccgagct gaacgcagct gcgcaaatcc gaggggcggc ctggtgccgc ccctttttct
     1021 tgctagagca atttctgccg cagcaggtcg gtggacttgc gccacaccca gccgacggtg
     1081 accagcgtca ccgctgcgcc cgcgagcacg gaaggcacca cgcccatcag ccgcgcggcg
     1141 gtgccggact cgaacgcgcc gagttcgttc gatgcgctga tgaagatgct gttcaccgcg
     1201 acgacccggc cgcgcaggtg gtcgggcggc aacgcctgca gcagcgtgct gcggatgatc
     1261 acgctcacgc tgtcgaacgc gccggtgagg aacagcgcgg ccatcgacag ccagatgttg
     1321 gtggagacgc cgaacaccag cgtggccgcg ccgaagcccg ccaccaccag cagcaggttg
     1381 cgccaagcgt gcgtcagcgg cggccaccag gcgcaggcga tcaccgtgat catggcgccc
     1441 accgcgggcg ccgcgcgcag caggccgagc ccctgcgcac cgacggccag gatgtcctgc
     1501 gcatacaccg gcaggatcgc caccacgccg ccgaacagca ccgagaacat gtcgagcgag
     1561 atggcgtaga ggatcacctt gttctgccgc acgtaggcaa tgccctcggc caggctggcc
     1621 cagatgttgt cgtgcgcttc cggcgccggc ggcgccggcc ggcgcgcgat gccgccgagc
     1681 agtgcgaggt tgagcaggat cagcccgatc accaccagca acgtgtgatg gatgcccagg
     1741 cccgcgtaga gcaggccggc cagcagcggc ccggcgatgg cgccggcctg ccagctggtg
     1801 ctgctccagg tcgccgagtt cccgtagtgc tcgcgcggca ccaggaacgg cttgagcgag
     1861 gccgtggccg gcgagaacac gccgcgcgca aagccgagca ggccgatgac ggcgtagcag
     1921 gtccaaacca gcgtgtcctg cgcaagccgc gcctgcgtgg cccaccacag cacgaccgag
     1981 ccgcacagga tcaccgacag gctggccagc atcagcccgc gcttctcgcg gcggtcggcc
     2041 aggtggccgc cgaacagcgc cagcgccatg aacgggatgg cctcggccag gccgagcagg
     2101 cccagcacca gcggatcgtg cgtgagcttg tacaggtcat acgacagcgc cacttcctgg
     2161 atcagcagcg cgatggtgtt gatgaaactg gcggcgagca ggaagcggaa ttctcggtag
     2221 cgcagcgccg cgaagggacc gccgctcacg tctgcgccac cgcgtgcgcc ttggtgtcga
     2281 ggaatttcac ctcggggaaa cgctcctgcg ccatcgacag gttcaccgaa ctcgacgcca
     2341 ggtacaccag gtcgccgtag tggtcgcgcg cgatgcgctg ctcgttcttc tcgacaaaat
     2401 ccttcagctt cttcgcatcg tcgcaataca cccagcgcgc gttgtgcacg gccacgttgt
     2461 cgaacaccga gtcgacgccg tattcgtcct tgagccggta ctgcaccact tcgaactgca
     2521 gcgcgcccac cgcgcccagc acgatgtcgt tgttggtcag cgggcgatac acctgggtgg
     2581 cgccctcttc gcacagctgg tcgaggccct tgttcagctg cttggccttg agtgggtcct
     2641 tcagcaccac gcggcggaac agctcgggcg cgaacgacgg gatgccggtg aagcgcagtt
     2701 cctcaccctc ggtgaacgag tcgccgatgg cgatggtgcc gtagttgtgc aggccgatga
     2761 tgtcgcccgg ccaggcctgc tccaccgcct cgcgctcggc cgccatgaag gtgagcgcgt
     2821 gcgacacgcg cacatgcccg cccaggcgca cgctgtgcag ctgcatgccc ttgcgatacg
     2881 tgcccgagca gatgcgcagg aaggcgatgc ggtcacggtg cttcgggtcc atgtttgcct
     2941 ggatcttgaa cacgaaaccg gtgaattttc cgtgcgccgg atcgatcggg tagtcctgtc
     3001 ccttgaactg cgcgatgcgc gcctgcggcg gcggcgccca ctcggtgaag ccgttaagca
     3061 gttcgcgcac gccgaagttg ttgatgcccg aaccgaagaa caccggcgtc agttttgctg
     3121 cgcgatattt atcgagatcg aaacccgtgc cggccatctg cagcagctcg atctcgtcgc
     3181 gcagcgtctg atacaccgcg ccgaagcgat cgcccaggcc gggcgagagc aggccgtcaa
     3241 tggtctcgat ctcgctgacg cgatgcttgt cgccgctgta caggtagaag cggtcttcca
     3301 gcaggtgata cacgcccttg aactcgcggc ccatgccgag cggccaggtg atcggcgtgc
     3361 attgcagccc gagcaccttc tccacttcgt cgagcagttc cagcggcggg cggccttcgc
     3421 ggtcgagctt gttgatgaag gtgatgatcg gcgtgtcgcg caggcggcac acctccatca
     3481 gcttgatggt gcgtgtttcg acgcccttgg cggcgtcgat caccatcagc gcgctgtcca
     3541 ccgcggtcag cgtgcgatag gtgtcctcgg aaaaatcctc gtgacccggc gtgtccagca
     3601 ggttcacgat gcagtcccgg tagggaaact gcatcaccga tgaagtcacg gagatcccgc
     3661 gctgctgctc gaggcgcatc cagtcggacg tcgcgtggcg cgccgccttg cggcccttga
     3721 ccgtgccggc caactggatg gcgccgccga acagcagcag cttttccgtc agcgtggtct
     3781 tgcccgcgtc cgggtgcgag atgatggcga aggtgcgccg cagcgcagcc tgttgtgcgg
     3841 cgtcgctcat agaaaggcgt tgctacaggg gccgcgattt taacggatca gcgccgggtt
     3901 ccgagaatga tgtcgcgcgc gcgcaggtcg gccaggatct tcgcgccctc gtccacgacc
     3961 cattcgggct gcggatgctg cagttcagcg gcaatccgca gcagcagctg gcgcccgctc
     4021 gatgcggcat cttcctccag cagattcatc agccgcgccg tcaccgcgtt cacttccagg
     4081 aatttcacat cgtcctggcg gttgcggttg acgatgagat aggtgggctg cgccggcttt
     4141 tcctgcggca ccgtggctgg accgatgcga tgca
//
LOCUS       DASZZC010000216         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_147709,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000216 DASZZC010000000
VERSION     DASZZC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147709"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="VHE37_04695"
     CDS             complement(<1..1482)
                     /locus_tag="VHE37_04695"
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HVT34853.1"
                     /translation="MSIPVVAVQDAPARYPLLGNVTERSEQRLTIERTLDLASDLFLR
                     DHTIGSAPSAHDPNLLPIPVLPFTFSMEIMAEAGACLLARDDLRLIGLEQCRGSRWLS
                     LDDGRLPLRITAERITAREGVERISGRIYVTGANAPAGGVLVFEATALYAVQYPQPPA
                     AKPWSSPDQNPARNNPDGELYKHGMFHGPRLQGVTHLRRWGEQSIEADLATIPTHDYF
                     SFTPSPQFQFDAALLDAAGQLAGYWLTEKHTWGFNCFPYRVGNLKLYAPPPRAGSHVL
                     CRAQLGFTDEHLLEAHFDLIGSDGQLIMRADRWEDRKFTMPARLYEYRLKPQERYLAQ
                     PWLAPTLPAGYIARRIEPFANQFLDEGGGIWKRMLAHMVLNQQERARYYLLPAAGPRR
                     EEWLMGRIAAKEALRDWALREHGMRLASADFEIASDELGRPFATCAQLPGRTLPAVSI
                     SHSRQWAVALLAPAVPRVGIDYQRLAGLDAALLEAGAFADDETR"
BASE COUNT          229 a          519 c          589 g          244 t
ORIGIN      
        1 tcttgtttcg tcgtcggcaa aggcgccggc ttcgagcagc gcggcatcga gcccggcaag
       61 gcgctggtag tcgatgccca cacgcggcac ggccggtgcc agcaacgcca ccgcccactg
      121 gcggctgtgg ctgatgctga cggccggcag cgtgcgcccc ggcagctgcg cgcaggtggc
      181 gaacgggcgg ccgagctcgt cgctggcgat ctcgaaatcg gccgacgcca ggcgcatgcc
      241 gtgttcgcgc agtgcccagt cgcgcagcgc ttccttggcg gcgatgcggc ccatcagcca
      301 ttcctcgcgg cgcgggccgg cggccggcag caggtaatag cgcgcgcgct cctgctgatt
      361 gagcaccatg tgcgccagca tgcgtttcca gatgccgccg ccctcgtcga ggaactggtt
      421 ggcgaatggc tcgatgcgcc gcgcgatgta gccggccggc agcgttggcg ccagccaggg
      481 ttgcgccagg tagcgctcct gcggcttgag gcggtattcg tacagccgcg ccggcatggt
      541 gaacttgcgg tcttcccagc ggtcggcgcg catgatcagc tggccgtcgc tgccgatcag
      601 gtcgaagtgc gcttccagca ggtgctcgtc ggtgaagccg agttgcgcac ggcacagcac
      661 gtgcgagccg gcgcgcggcg gcggcgcata cagcttgagg ttgcccacgc ggtaggggaa
      721 gcagttgaag ccccaggtgt gcttctcggt gagccagtaa ccggcgagct ggccggcggc
      781 gtcgagcagc gcggcgtcga actggaactg cggcgacggc gtgaagctga agtagtcgtg
      841 cgtgggaatc gtcgccaggt cggcctcgat cgactgctcg ccccagcggc gcaggtgggt
      901 gacgccctgc aggcgcgggc cgtggaacat gccgtgcttg tagagctcgc catcgggatt
      961 gttgcgcgcg ggattctggt ccggcgacga ccacggcttg gccgccggcg gctgcgggta
     1021 ttgcaccgcg tacagcgcgg tcgcttcgaa caccagcacg ccgccggccg gcgcgttggc
     1081 tccggtcacg tagatgcggc cggaaatgcg ctccacgccc tcgcgcgcgg tgatgcgctc
     1141 ggcggtgatg cgcagcggca ggcggccatc gtccagcgac agccagcgcg agccgcggca
     1201 ctgctccagc ccgatcagcc gcaggtcatc gcgcgccagc aggcaggcgc cggcctcggc
     1261 catgatctcc atgctgaagg tgaacggcag caccgggatc ggcagcaggt ttggatcgtg
     1321 cgcgctcggc gcgctgccga tggtgtgatc gcgcaggaac aggtcggagg cgaggtcgag
     1381 cgtgcgctcg atggtcagcc gctgttcgct gcgttcggtg acattgccca gcagcggata
     1441 gcgcgcaggc gcgtcctgga cggccaccac tggtatcgac atggaaggcg cgggcgcgga
     1501 cggcaccacc gccgcctgcg gctggcccga cagcaggctg agcatgcgcg cctggctgtc
     1561 gagaaattcc tgcatcagtg c
//
LOCUS       DASZZC010000217         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_147880,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000217 DASZZC010000000
VERSION     DASZZC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147880"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="ribD"
                     /locus_tag="VHE37_04700"
     CDS             <1..1064
                     /gene="ribD"
                     /locus_tag="VHE37_04700"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HVT34854.1"
                     /translation="SAQGGVDSPVPADLLFMGRALELAACGLYSARPNPAVGCVIVRN
                     GVVVGEGWHERAGGPHAEVHALAQAGERARGSDMYVTLEPCSHHGRTPPCADAVIAAG
                     ISRVRVAMQDPNPKVSGAGIARLRAAGLQVEVGGEFEEGVRDLNRGFISRMTRGRPWV
                     TLKLAASADGRTALASGESRWITGPDARADVHRMRARAGAVLTTSATVLADDPELTVR
                     DFVPPPGVELRQPDRIVLDRNRRVPKSAKVWREDGARTFHITDGDLHSAMKNLAQQEV
                     NEVLVECGPRFAGALLQAQLVDELVLYLAPSLLGDAARPLAYLPGIDTLAQRVQLHWV
                     EACKIGADLRLTAVTAPPF"
     gene            1137..1742
                     /locus_tag="VHE37_04705"
     CDS             1137..1742
                     /locus_tag="VHE37_04705"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HVT34855.1"
                     /translation="MFTGIIEATGTLQQVAETGGDRRMTFVCAGPLLGAVKLGDSIAV
                     NGVCLTATQHVAQGFVADLSAETLAATTAGAWRTGMAVNLELALTPAKPLGGHFVSGH
                     VDGVGQLIEKHEDARSWRYTFEAPAALARYIARKGSITIDGVSLTVNEVSGRQFGVNI
                     IPHTLQATTLGTLVAGAQVNLEVDLVARYLERLLAAREEGT"
     gene            1739..>1958
                     /locus_tag="VHE37_04710"
     CDS             1739..>1958
                     /locus_tag="VHE37_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HVT34856.1"
                     /translation="MNAKVTSIENRENRAPGLDRIEDIIADIKAGKMVILMDDEGREN
                     EGDLIMAAAQVRAEDINFMARYGRGLICL"
BASE COUNT          332 a          655 c          671 g          300 t
ORIGIN      
        1 tgagcgcgca gggtggcgtc gactccccgg ttccggccga cctgcttttc atgggacggg
       61 cgctggaact cgctgcctgc ggcctgtact ccgctcgccc caatcccgcg gtcggttgcg
      121 tcatcgtgcg caacggcgtc gtcgtcggcg aaggctggca cgagcgcgcc ggcggcccgc
      181 acgcggaagt gcacgcactg gcgcaggccg gagaacgcgc gcgtggctcg gacatgtacg
      241 tcacgctgga accgtgcagt caccacggcc gcacgccgcc gtgcgccgat gcggtgatcg
      301 cagcgggcat cagccgggtg cgcgtcgcga tgcaggatcc caaccccaag gtttccggtg
      361 ctggcattgc gcgcctgcgt gccgcaggac tccaggtgga ggtcggcggc gaattcgagg
      421 agggcgtgcg cgacctcaac cgcggcttca tctcgcgcat gacgcgcggc cggccgtggg
      481 tcacgctcaa gcttgccgcc agcgccgacg gccgcacggc cctggccagc ggcgaaagcc
      541 ggtggatcac tggacccgac gcccgcgcgg acgtgcatcg aatgcgggcg cgcgccggcg
      601 ccgtgctgac gacgagcgca accgtactgg ccgacgaccc cgaactcacc gtgcgcgatt
      661 tcgttccacc gcccggcgtg gagctgcgcc agcccgaccg catcgtgctc gaccgcaacc
      721 ggcgcgtgcc caaatcggcc aaggtgtggc gcgaggatgg cgcgcgcact ttccacatca
      781 ccgacggcga cctgcattcg gcgatgaaga acctggcgca gcaggaggtc aacgaggtgc
      841 tggtggaatg cggcccgcgc ttcgccggcg cgttgctgca ggcgcaactg gtggacgagc
      901 tggtgctgta ccttgcgccg agcctgcttg gcgatgccgc gcggccgctg gcgtatctgc
      961 ccggcatcga cacgctggcg cagcgggtgc agctgcactg ggtggaggcg tgcaaaatcg
     1021 gcgcggatct gcgcctgacg gccgtcaccg caccgccatt ttgagtgtta ttaattatta
     1081 tatgttttta aaatttatta acaaattaaa acacacccaa acggggtgcg gttagcatgt
     1141 tcacgggcat catcgaggcc accggcacgc tgcagcaggt tgccgaaacc ggcggcgacc
     1201 gccgcatgac cttcgtctgc gccggcccgc tgctcggcgc cgtgaagctc ggcgacagca
     1261 tcgcggtgaa cggcgtgtgc ctcaccgcca cgcagcacgt ggcgcaggga ttcgttgcgg
     1321 acctgtcggc ggaaacgctc gccgccacca ccgccggcgc ctggcgcacc ggcatggcgg
     1381 tgaacctgga actggcgctc acgccggcca agccgctggg cgggcatttc gtttccggcc
     1441 atgtcgacgg cgtcggccag ctgatcgaaa agcacgagga cgcgcgatcc tggcgctaca
     1501 ccttcgaggc gccggcggcg ctggcgcgct acatcgcacg caagggctcc atcaccatcg
     1561 acggcgtcag cctgaccgtc aacgaggttt ccggccggca gtttggggtt aacatcattc
     1621 cgcacacctt gcaggccacc acgctcggca ccctggtcgc cggcgcgcag gtcaacctgg
     1681 aagtggatct ggtcgcgcgc tacctggaac ggctgctcgc ggcgcgggag gaaggaacat
     1741 gaatgcaaaa gtaacgtcga tcgaaaatcg ggagaaccgc gcgccgggcc tcgaccgcat
     1801 cgaggacatc attgccgaca tcaaggccgg caagatggtg atcctgatgg acgacgaggg
     1861 ccgcgagaac gagggcgacc tgatcatggc cgctgcgcag gtgcgcgcgg aagacatcaa
     1921 cttcatggcg cgctacggcc gcgggctgat ctgcctca
//
LOCUS       DASZZC010000218         8031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1237899,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000218 DASZZC010000000
VERSION     DASZZC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237899"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VHE37_04715"
     CDS             complement(<1..498)
                     /locus_tag="VHE37_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT34857.1"
                     /translation="MLGMRMRSVCLMLPALLLLPAAAQANDLLKIYQEARDNDAQLQA
                     AEHARDAGIEARPIARGDLLPQITGSAAESREHDKISSDSLGIDPTTGLPIAGSATRN
                     SSPWSYGITLTQTVFSWQQISALRQAGDEAALAELTYRSAEQDLRLRVAQAYFNVLAA
                     LDTLRS"
     gene            complement(517..828)
                     /locus_tag="VHE37_04720"
     CDS             complement(517..828)
                     /locus_tag="VHE37_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVT34858.1"
                     /translation="MRNLSPGDIAADVTAGKLPLIDVRTAEELAIVRLPQARHIPMDQ
                     IPARLADLNPQQPVAILCHHGGRSLQVAHYLERNGFADVINVAGGIDAWADLDPTLPR
                     Y"
     gene            complement(868..1869)
                     /locus_tag="VHE37_04725"
     CDS             complement(868..1869)
                     /locus_tag="VHE37_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT34859.1"
                     /translation="MDGDSKKRSAEPAAPVSAIDPVDRFIARLYRSGLSVSGDFRQWA
                     LQELAHVVPHDGALWGSGIFAERRFHSYTLTNLPPSFPATLEATTQVNPLIPAIVGNL
                     DCPVDSRLVVPDRQFFNSEIYKQAFAPHGIARILATAHLDRRSGLYSMVSIYRRDRAN
                     IFSEAEKAQQKHIVFHMFNAASHAFFLRLVRERGRPAGSAAAVVDRMGCFHQVQPQFL
                     DLLEAHFPPKNPPVLPFPLPAVGETEHIGPLCARAEPSADMFLVYLWKAGPLDKLTQR
                     EREIVFAVAQGLSFKQAAKKIGLAPSTVANHLYRVYRKLGVYSRTELAQLVHPQSES"
     gene            1920..2399
                     /locus_tag="VHE37_04730"
     CDS             1920..2399
                     /locus_tag="VHE37_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HVT34860.1"
                     /translation="MKTLRNWLNEYSESHQNPRNKLLHWLCIPQIVFAVFLALKTLPA
                     GDALINPATIAAVLALAYYARLSWQLAIGTALLFGLMYAVILAITGALGSMWSLGLAA
                     AIFVEAWVGQFIGHNIEGARPSFFKDLQFLLIGPLWLLADVYRRMAIPVGKQPAAQS"
     gene            2566..5199
                     /locus_tag="VHE37_04735"
     CDS             2566..5199
                     /locus_tag="VHE37_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34861.1"
                     /translation="MTVASRRAFALPVSALLLLAACHSSEPLGTAGGGSGATTGSATG
                     DTTGSSTGSTTGGTTGGTTGGVDNSPPPQQGSLDSSVAVDVAKATVQGCDFLDTHYCM
                     FPWPSDQFTTADDTTDTGKRINLSLLAMPRNVAGKPLVPTEWNRNDGFSVGQMILLRV
                     PNLDLAKTGAVPITNVGDSFRDDQPIVVLDVGPAKTPDPNYVPQRHLIYSELDANITK
                     FTACDAPKPAAVLAGLLSDAGLPGLDQVAAAAQSLRDACKQFPIPENPLVDPGPALQI
                     RPAVNFQEGHRYIVALRNLKDGDGNLIDAPASFQIYRDNHSSSLPMVEGRRAHFEDLF
                     TTLGKAGIKRSELYMTWDFTVISQRSLTERVLHIRDDALAKLGSNAPVITKVTHVDNV
                     GDSKIAREVRGVIEVPSYLSIPHGSAGSYFYYQPDASGVYGDGLPDVNPLQPTQTFDF
                     LCRIPRRAFGNVSDPATATSAEQERPALYGHGLLGSKSEGSGQIGDIIQQDGMIYCAT
                     DWIGMASHDSFDPANGQVVDTVYYDPPEGDVLNVLTLLLDMSNFGTLTDRVQQSLVNF
                     TYLGRAVINPAGFCALPEFKVGDTCLIDTSELYYDGNSQGGIIGGALMAISPDIKAGV
                     LGVPGMNYSTLLQRSVDFDTYAAFMYASYQASLDQQFVLSMVQMLWDRAENDGYAHHL
                     SATDHLPGTPPKRILMHPAFGDHQVSMYTAEVMARTIGAKVHCPAVVGGSDSTQRGPA
                     VEHFPNAAVVKEQEVLPALSKGRRHPDDEPYYGIPCMDESAPYAGNAMVIWDSGPTVH
                     IDGSPRNDDGGDPSGVAPPPTDNTPPRPQLGYGGDPHEFPRSTDASRRQKDDFYRHNG
                     GVVDKCGGRPCGTRGFDQDIQ"
     gene            5256..>8031
                     /locus_tag="VHE37_04740"
     CDS             5256..>8031
                     /locus_tag="VHE37_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVT34862.1"
                     /translation="MYRNIAVAATAALAFFGMALVVTAAAPRNFDSPASHDTSQQFAS
                     GSVQRQDTPNDPDYDKAEPDDEDGAPATTNLYDERWDLFGFPSQLTPLAVYKDPSDSL
                     RFGQPMVSGYNAAGAWKLTRGRGDVVIAILDTGIRWEKGELRRQVHLNCAELPAPHNA
                     AGVAVASANASPGCKEPDFVYDLNGNGYLDVDDYQDDARVDKNGGAHGIKDTIDAEDL
                     IVAFSDGDDDDHNGYVDDIAGWDFFNNDNDPYDQSSYFAASNHGSGRASDAVEQGNEG
                     SGGLGVCPECQFVPLRVWDTFVADPNNFGLAVVYAADNGVSVIEGADGALGHTAFMEA
                     ASEYAYRKGLVQTFSGDDLNTGNHNYPANYSHVMLIQGTVPDSMGLGENAPSQAAAVL
                     AQIPVPVGTQIPVLTYFRGANTTQYGGHSSISMEGATGSENTGKAAGAAGMVISAARS
                     AGTALRPDEARAILEQTAEDITEPNTAGLGLADATQPGWDTHFGWGRVNLGAAVSLAA
                     SAKVPPEAAITAPDWYAPLTGKTLHVSGVARARFATGGAFHWKLEWGAGIAPTSFTTV
                     REGDSNASVTDFGDIDLDAVRSALTSAPALALDPGAPVFAPGAVTPYDAQFAVRLTVT
                     GTGLDVPGVDRRVFTAFNDATLRAGYPLRLGAGGEAPLRYADLDGDNVPELIVPTEDG
                     VLRVLKKDGSELAGFPVYTQLQMQALNHQAAPGVAALMTATPPREDLRGPAVGDLDGD
                     GIPEIIDAAGIHVYAWNHDGTLHAGFPVSLDLNNCKGADQKQDNVHRKCGFIGSPALG
                     HLEGKDHGLDIVIAGLDGHLYAWRGDGSALPGFPVNLVDPAQSADQQVLAESINNAAV
                     GDLDGDGFDDVVIATNETYAAENPSPSDLPGLAAAAFADALAQAAGSSSRVYAVSGKD
                     GSLLPGWPLHL"
BASE COUNT         1424 a         2682 c         2636 g         1289 t
ORIGIN      
        1 ggaacgcaac gtgtccaatg cagccagcac gttgaagtag gcctgcgcga cgcgcaggcg
       61 gagatcttgc tccgccgagc gataggtgag ctccgcgagc gccgcttcat cgccagcctg
      121 gcgcagcgca gaaatctgct gccagctgaa tacggtctgc gtcaacgtga tgccgtagct
      181 ccacggcgac gagttgcgag tggcagatcc tgcgatcggc aagccggtcg ttggatcaat
      241 gcccaagctg tcagagctga tcttgtcgtg ttcacgcgat tcggcggcgc tgccggtgat
      301 ctgcggcagc aggtcgccgc gggcgatggg cctggcttcg atgccggcat cgcgcgcgtg
      361 ttcggctgcc tgcagctgcg catcattgtc gcgcgcttcc tggtagatct tgagcaggtc
      421 attggcctgg gccgcggccg gcaacagcag cagggccgga agcatcagac agactgagcg
      481 catgcgcatc cccaacatga ttgtttggtt gttgattcag tatcgcggca gcgtagggtc
      541 gaggtccgcc caggcatcga tgccgccggc gacgttaatg acatcggcaa agccattccg
      601 ttccaggtag tgcgccacct gcaggctgcg cccgccgtgg tgacatagga tggcgaccgg
      661 ctgctgcggg tttaaatcgg cgagccgcgc aggaatctgg tccatcggga tatggcgggc
      721 ctgcggcagg cgcacgatgg cgagttcctc ggcggtgcgg acgtcgatca gtggcagctt
      781 gcccgccgtc acgtcggccg cgatgtcgcc aggtgaaagg ttgcgcatgc tcaacagtat
      841 gaaaatgggc ggcgcgcagt gtaactgcta ggactcgctt tgcgggtgaa ccagttgcgc
      901 cagttccgtg cggctgtaga cgcccagctt gcgatacacg cggtacaggt ggttggccac
      961 ggtggacggc gccagaccga ttttcttcgc cgcctgcttg aacgacagcc cctgcgccac
     1021 tgcaaacacg atttcgcgct cgcgctgtgt cagcttgtcc agcgggccgg ccttccacag
     1081 gtacaccagg aacatgtccg cgctgggttc tgcgcgcgcg caaagcgggc cgatgtgctc
     1141 ggtctcgccg acagccggca gcgggaacgg cagcaccggg ggattcttgg gtggaaagtg
     1201 cgcttcgagc aggtcgagga actgcggctg cacctggtgg aagcagccca tgcggtccac
     1261 cacggccgct gcgcttccag ccgggcggcc gcgctcgcgg accaggcgca ggaagaaggc
     1321 atgcgaagct gcgttgaaca tgtggaacac gatgtgcttc tgctgtgcct tctcggcctc
     1381 gctgaagatg ttggcgcggt cgcgccggta gatcgacacc atcgaataca ggccgctgcg
     1441 gcggtccagg tgcgcggtgg cgagaatgcg cgcaatgccg tggggtgcga aggcctgctt
     1501 gtagatttcc gaattgaaga actgccggtc gggtaccacc aggcgcgaat ccaccgggca
     1561 gtccaggttg ccgacgatgg ccggaatcag cggattgacc tgcgtggtcg cctccagcgt
     1621 cgccgggaaa ctgggaggaa ggttggtcag cgtgtagctg tggaagcgcc gctcggcgaa
     1681 gatgccgctg ccccacaggg cgccatcgtg cggaaccacg tgtgccagtt cctgcaatgc
     1741 ccactggcgg aagtcacctg acaccgacag gccggagcga tacagccgcg cgatgaagcg
     1801 atcgaccgga tcgatggcgc tgaccggcgc agcgggttct gcggagcgct ttttactatc
     1861 gccgtccatg agttacgcga gcatactccg tcgccgcaaa ttcttcagtc ggaaagtcca
     1921 tgaaaacgct tcggaactgg ctcaatgagt attccgagag ccaccagaat ccgcggaaca
     1981 agctgctgca ctggctgtgc attccgcaga tcgtgttcgc ggtgttcctg gcgcttaaga
     2041 ccctccctgc cggcgatgcg ctgatcaatc ccgccacgat agccgcggtg ctcgccctgg
     2101 cctattacgc gcggctttcc tggcagctgg ccatcgggac cgcgctgctg ttcgggctga
     2161 tgtacgcggt gatcctcgcc atcaccggcg ccctcggcag catgtggtca ctcggactgg
     2221 ccgcggcgat tttcgtcgaa gcctgggtcg ggcagttcat cggccacaac attgagggcg
     2281 cccgcccgtc cttcttcaag gatctgcagt tcctgctgat tggcccgctg tggctgctgg
     2341 ccgacgtcta tcgccgcatg gcgatcccgg tgggcaagca gcccgccgca caatcctgaa
     2401 gccctgcggc tttacctttc tttgccctgc cggatgcggc cgggcggcat gcggctggct
     2461 agacaatagc catcaagacc gggaaacccg aacccggcgg tgatttaatt ccagtgcatt
     2521 agaaggcggg ccagaaccgc cgcgcatgat gatgaggagc gtcacatgac cgttgcatct
     2581 cgccgcgcgt ttgcgctgcc ggtatccgca ttgctgctgc tggccgcatg ccattcgagc
     2641 gagccgttgg gaacggccgg cggcggcagc ggcgccacca ccggcagtgc caccggcgat
     2701 acgactggaa gcagcacggg aagcactacg ggcggaacga ctggcggcac tacaggcggc
     2761 gtggacaact ctccgccgcc gcagcagggc agcctggaca gcagcgtggc ggtcgacgtg
     2821 gccaaggcca ccgtgcaggg ctgtgatttt ctcgacacgc actactgcat gttcccgtgg
     2881 ccgagcgacc agttcaccac ggccgacgac accactgata ccggcaagcg catcaatctc
     2941 agcctgctgg ccatgccgcg caacgtggcc ggcaagccgc tggtccccac cgagtggaac
     3001 cgcaacgacg ggttctccgt cggccagatg attctcctgc gggtgccgaa tctcgatctg
     3061 gcgaagaccg gcgccgtgcc catcaccaac gtcggcgaca gcttccgcga cgatcagccg
     3121 atcgtggtgc tcgacgtcgg tccggcgaag acgcccgatc cgaattacgt gccccagcgt
     3181 cacctgatct acagcgagct cgacgccaac atcaccaagt tcaccgcctg cgatgcaccc
     3241 aagcccgccg cagtgctggc aggactgctg tccgatgccg gattgccggg gctcgaccag
     3301 gtcgccgccg ccgcgcagtc actgcgcgat gcctgcaagc agttccccat cccggaaaat
     3361 ccgctggtag atccaggccc ggcgttgcag atccgccccg cggtcaattt ccaggaaggg
     3421 caccgctaca tcgtggcgct gcgcaacctc aaggatggcg acggcaacct gatcgacgcg
     3481 ccggcctcgt tccagatcta ccgcgacaac cacagcagca gcctgccgat ggtggaaggg
     3541 cggcgcgcgc atttcgaaga cctgttcacc acgctgggca aggccggcat caagcgcagc
     3601 gagctgtaca tgacctggga cttcaccgtg atcagccagc gctcgctgac cgagcgcgtg
     3661 ctgcatatcc gcgatgacgc gctggccaag cttggcagca atgcgccggt gatcaccaag
     3721 gtcacgcatg tcgacaatgt gggcgacagc aagatcgcac gtgaggtgcg cggcgtgatc
     3781 gaggtgccgt cctacctgtc gatcccgcat ggcagcgccg gttcgtattt ctattaccag
     3841 cccgacgcca gcggcgttta tggcgacggc ctgcccgacg taaacccgct gcagcccacg
     3901 cagaccttcg attttctctg ccgcattccg cgccgcgctt tcggcaatgt gagcgatccg
     3961 gcgaccgcca ccagcgccga gcaggagcgc cccgcgctgt acgggcatgg attgctcggc
     4021 tccaagagtg aaggcagcgg ccagatcggc gacatcatcc agcaggacgg gatgatctac
     4081 tgcgccaccg actggatcgg catggcctcg cacgattcgt tcgatccggc caacgggcag
     4141 gtggtcgaca ccgtctacta cgatccgccg gaaggcgacg tgctcaacgt gctcacgctg
     4201 ctgctcgaca tgagcaactt cggcacgctg accgaccgcg tgcagcagag cctggtcaac
     4261 tttacctatc tgggccgtgc ggtgatcaac ccggccggct tctgcgcgtt gccggagttc
     4321 aaggtgggcg atacctgcct gatcgacacc agcgagctgt actacgacgg caactcgcag
     4381 ggcggcatca tcggcggtgc gctgatggcc atttcgcccg acatcaaggc cggcgtactg
     4441 ggcgtgcccg gcatgaacta ctccacgctg ctccagcgca gcgtggactt cgacacgtat
     4501 gccgcattca tgtacgcctc ttaccaggcc tcgctcgatc agcagttcgt gctgtcgatg
     4561 gtccagatgc tgtgggaccg cgcggagaac gacggttacg cgcatcacct gtcggcgacg
     4621 gatcacctgc ccggcacccc gcccaagcgc atcctgatgc atcccgcatt cggcgatcac
     4681 caggtgagca tgtacaccgc cgaagtgatg gcgcgcacca tcggcgcgaa ggtgcattgc
     4741 ccggccgtgg ttggcggctc cgacagcacg cagcgcgggc cggcggtgga acatttcccc
     4801 aacgcagcgg tggtgaagga acaggaagtg ctgccggcat tgtcgaaggg ccggcgccat
     4861 ccggacgatg agccgtacta cggcattccg tgcatggatg aaagcgcgcc gtacgccggc
     4921 aacgccatgg tgatctggga cagcggcccg acggtacaca tcgacggcag tccgcgcaac
     4981 gacgacggcg gcgatccttc cggtgtcgcg ccgccgccca ccgacaacac gccgccacgg
     5041 ccccaactgg gctacggcgg cgacccgcac gagttcccgc gcagcaccga tgccagccgc
     5101 cggcagaagg acgatttcta tcgccacaac ggcggagtgg tggacaagtg cggcggcagg
     5161 ccttgcggca cccgagggtt cgaccaggac atccagtagc gcagcagaca gtcgccgcca
     5221 ataacaacta cacgggcggc ggcagaggag gagccatgta caggaacatc gcggtggcgg
     5281 cgacagcagc gctggcgttt ttcggcatgg cgctggtcgt gactgccgcc gcgccgcgca
     5341 actttgacag cccggcgtcg cacgacacct cgcagcaatt cgccagcggc agcgtgcagc
     5401 ggcaggacac gcccaacgac ccggactacg acaaggccga gccggacgac gaggacggcg
     5461 cacccgccac caccaacctg tacgacgagc gctgggacct gttcggcttt ccctcgcagc
     5521 tcacgccgct ggcggtttac aaggacccca gcgacagcct gcgcttcggc cagccgatgg
     5581 tttccggcta caacgccgcc ggcgcgtgga agctcacgcg cgggcgcggc gacgtggtca
     5641 tcgccatcct cgacaccggc atccgctggg agaagggcga actacgccgc caggtgcacc
     5701 tgaactgcgc cgagttgccg gctccgcaca atgccgcggg tgtcgccgtg gcttcggcca
     5761 acgcctcacc cggctgcaag gagccggact tcgtttacga tctgaacggc aacggctatc
     5821 tcgacgtcga cgactaccag gacgatgccc gcgtcgacaa gaacggcggc gcgcatggca
     5881 tcaaggacac catcgacgcc gaagacctga tcgtcgcctt ctccgacggc gacgacgacg
     5941 accacaatgg ctacgtcgac gacatcgccg gctgggattt cttcaacaac gacaacgatc
     6001 cctacgacca gtccagctac ttcgccgcgt cgaaccacgg cagcggccgc gccagtgatg
     6061 cggtggagca gggcaacgag ggcagcggcg ggctcggcgt gtgccccgaa tgccagttcg
     6121 tgccgctgcg cgtgtgggac accttcgtcg ccgatccgaa caacttcggc ctcgccgtgg
     6181 tgtacgcggc ggacaatggc gtcagcgtga tcgaaggcgc cgatggtgcg ctcggccaca
     6241 ctgcgttcat ggaagccgcg tcggaatacg cgtaccggaa aggcctggtg cagaccttct
     6301 ccggcgacga cctcaacacc ggcaatcaca actacccggc caactacagc cacgtgatgc
     6361 tgatccaggg cacggtgccc gactcgatgg gcctgggcga gaacgcgccg tcgcaggccg
     6421 cggcggtgct ggcgcagatc ccggtgccgg tgggcacgca gattcctgtg ctcacttatt
     6481 tccgcggcgc caacaccacg caatacggcg gacacagctc gatctcgatg gaaggcgcca
     6541 ccggctcgga gaacaccggc aaggctgccg gcgccgccgg catggtgatc agcgctgcgc
     6601 gcagcgccgg caccgcgctg cgtcccgacg aggcgcgcgc cattctcgaa cagactgcgg
     6661 aggacatcac cgagccgaac accgcgggcc tcgggctggc cgacgccacg cagcccggct
     6721 gggacacgca tttcggctgg ggccgcgtga atctcggcgc ggccgtgtcg ctggcggcct
     6781 cggccaaggt gccgccggaa gcggcgatca ccgcgcccga ctggtacgcg ccgcttaccg
     6841 gcaagaccct gcacgtcagc ggcgtcgcgc gcgcgcgctt cgccaccggc ggcgcgttcc
     6901 actggaagct cgagtggggc gccggcatcg cgccgacttc gttcaccacc gtgcgcgaag
     6961 gtgattcaaa cgcatccgtc accgacttcg gcgacatcga tctcgacgcc gtgcgcagcg
     7021 cgctgaccag cgcgcccgca ttggcgctcg atccgggcgc gccggtgttc gcgcccggcg
     7081 cggtcacgcc gtacgacgcg cagttcgccg tgcgcctgac cgtgaccggc accgggctgg
     7141 atgtgcccgg cgtcgatcgc cgcgtcttta ccgcgtttaa tgacgccacg ctgcgcgccg
     7201 gctacccgct gcgcctgggc gcgggcggcg aggcgccgct gcgctatgcc gatctcgacg
     7261 gcgacaacgt tcccgaactg atcgtgccga ccgaagacgg cgtgctgcgc gtgctgaaga
     7321 aggacggcag cgaactggcc ggctttccgg tgtacacgca gctgcagatg caggcgctga
     7381 atcaccaggc ggcgccggga gtggctgcgc tgatgacggc gacgccgccg cgcgaggact
     7441 tgcgcggccc cgcggtcggc gatctcgacg gcgacggcat cccggaaatc atcgacgccg
     7501 ccggcatcca cgtgtacgcg tggaaccacg acggcacgct gcacgccggt ttcccggtat
     7561 cgctggatct caacaactgc aagggcgccg accagaagca ggacaacgtg caccgcaagt
     7621 gcggcttcat cggcagcccg gcgctcggcc acctcgaagg taaggaccac gggctcgaca
     7681 tcgtcatcgc cggcctcgac gggcacctgt acgcctggcg cggcgacggc agtgcgttgc
     7741 ctggcttccc ggtgaacctg gtcgatcccg cgcagtccgc cgaccagcag gtgctggccg
     7801 agtcgatcaa caacgcggcg gtcggtgatc tcgatggcga tggtttcgac gacgtggtca
     7861 tcgccaccaa cgaaacctat gccgcggaaa atcccagccc gagcgatctg ccgggtctgg
     7921 ccgccgcagc cttcgccgat gcgctggcgc aggccgcggg cagttcgtcg cgcgtgtacg
     7981 cggtgagtgg caaggacggc agcctgctgc cgggctggcc gctgcatctg a
//
LOCUS       DASZZC010000219         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_20113,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000219 DASZZC010000000
VERSION     DASZZC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20113"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VHE37_04745"
     CDS             <1..735
                     /locus_tag="VHE37_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVT34863.1"
                     /translation="LQLPAAYLTCNFTPPLTGKPPLLTHDEVLTLFHEFGHGLHHLLT
                     RVDEAAVSGIRGVAWDAVELPSQFMENWAYDPATLRGFARHWQSGAVMPEALIGKLRA
                     SRTFHAALGTLRQVEFALFDLRLHRDYDSAKGPRVLETLKAVRDEVAVMQPPEWNRMP
                     CSFGHIFAGGYAAGYYSYKWAEVLSADAFAAFEETGFAPETGQRFRDTVLAQGGSKEA
                     MDLFVEFRGRKPSVEPLLRQHGLVAG"
     gene            complement(903..1679)
                     /locus_tag="VHE37_04750"
     CDS             complement(903..1679)
                     /locus_tag="VHE37_04750"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVT34864.1"
                     /translation="MRLQGKTAVITGGARGMGAATARLFAAEGARVLIGDVLDADGEK
                     LARELGPAASYRHHDVTDEASWHALIDAAKSAHGGIDVLVNNAGVLLFKTIADTSKAD
                     FERVLGVNLVGTFLGTRLVGAHMVARGRGSIVNISSADGMKGANGLGAYCASKWGSRG
                     LTKVAAMEFGHKGVRVNSVHPGGIDTAMGNPYAEKREDVNKRYGMVPLQRVGDPSEVA
                     RTSLFLASDESSYLCGAEIAVDGGMLCGQYYVGFPGAPGV"
     gene            1793..>3147
                     /locus_tag="VHE37_04755"
     CDS             1793..>3147
                     /locus_tag="VHE37_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT34865.1"
                     /translation="MNATLHHSRNLLHRYGLALLAPVALMLLRDVLVNAGLIGGALTL
                     PFFVIAAAIAGGLGGFWAGMAATAFGGFLLSQYVYPGDGHEAMALRLLNLFLLGLTVS
                     VVAGAQQRASRRAQALADNAARRAADLQDINDRLSRQERSLLQAQRVAHFGTWEVDVK
                     LGINRFSDELCVLIGIEPGTPLDFAAASALTYPEDIARVDASFRNCLAQREAVWRVEH
                     RRLHPQRGLIWLEVRAEVAYDEQGRVTRMIGATMDVTERRRAEDALRASERMLSLIYE
                     NSRDALYLAQVESAERYVLVSANQAFLKVSGYRRDQVIGRPMEEVMPSLSNHAKVRAQ
                     YQRAIEARRAIVYEEIADLPAGQKYGEIHLIPIFKEDGPVTHILGAIKDITDQRRAQK
                     KLRESEEQFRRAMEEAPIPVIMCADDGEVLQVSKTWVQLTGFALADVPTFESWLGKSY
                     G"
BASE COUNT          485 a         1084 c         1071 g          507 t
ORIGIN      
        1 ctgcagctgc cggcggcgta tctcacctgc aatttcacgc caccgttgac cggcaagcct
       61 ccgctgctca cgcacgatga agtgctcacg ctgttccatg aattcggcca tggcctgcat
      121 cacctgctca cgcgcgtgga cgaggctgcg gtatcgggca tccgcggcgt ggcctgggat
      181 gcggtggaac tgcccagcca gttcatggag aactgggcct acgatcccgc cacgctgcgc
      241 ggctttgcgc gccactggca gagcggcgcg gtgatgcccg aggcgctgat cggcaagctg
      301 cgcgcgtcgc gcactttcca cgcggcgctc ggcacgttgc gccaggtgga gttcgcgctg
      361 ttcgacctgc gcctgcaccg cgactacgat tccgcgaaag ggccgcgcgt gctggaaacg
      421 ctcaaggccg tgcgcgatga agtcgccgtg atgcagccgc cggaatggaa ccgcatgccg
      481 tgcagcttcg gccacatctt cgccggcggt tacgcggcgg gttactactc gtacaagtgg
      541 gcggaagtgt tgtccgccga tgccttcgcc gcgttcgagg agactggttt tgcgccggag
      601 accgggcagc gcttccgcga cacggtgctg gcccagggcg gctcgaagga ggcgatggat
      661 ttgttcgtcg agttccgcgg gcgcaagccg agcgtggagc cgctgctgcg gcagcatggg
      721 ttggtggcgg ggtgagcgac gcgaactccg cgagcggctt cagcatgcat tccctcttcc
      781 cttgcgggag agggctaggg tgcagcgcgt tgatctgttc gcttcacacg tgcgtgttga
      841 gtgagtagat gatgaaactc tccggctcag cgccgaagcg cgcgccgcgc ccgcaaccat
      901 cctcataccc ccggcgcccc cggaaacccc acgtaatact gtccgcacag catcccgcca
      961 tccaccgcga tctccgcgcc gcagagataa gaggactcat cggacgccag gaacaggctg
     1021 gtccgcgcca cctccgacgg atcgcccacg cgctgcagcg gcaccatgcc gtagcgcttg
     1081 ttcacgtcct cgcgcttctc ggcgtaaggg ttgcccatcg cggtgtcgat gccgcccgga
     1141 tggaccgagt tgacgcgcac gcccttgtgg ccgaattcca tcgcggccac cttggtcagc
     1201 ccgcggctgc cccacttgct ggcgcagtag gcgcccaggc cattggcacc cttcatgcca
     1261 tcggccgagg agatgttgac gatcgacccg cggccgcgcg ccaccatgtg cgcgcccacc
     1321 agccgggtgc cgaggaaggt gccgaccagg ttcacgccga gcacgcgctc gaaatccgcc
     1381 ttgctggtgt cggcgatggt cttgaacagc agcacgccgg cgttgttgac cagcacatcg
     1441 atgccaccgt gtgcggactt cgcggcgtcg atcagcgcgt gccagctggc ctcgtcggtc
     1501 acgtcgtggt gccggtagct ggcggccggg ccgagctcgc gcgccagctt ctcgccgtcg
     1561 gcatcgagca cgtcgccgat gagcaccctg gcgccttctg cggcgaacag ccgcgccgtg
     1621 gccgcgccca tgccgcgtgc gccgccggtg atgaccgctg tcttgccctg cagacgcata
     1681 tgcgctcctc cgtgtaattg ctctgcgacg attgcaccgc gggcggcgcc gggcctcgtc
     1741 cgaacgcagg actgccggtg ctaacatggc ccgcacatcg agacgctcac ggatgaacgc
     1801 gacgctgcac cattcccgca acctgctgca ccgctacggc ctggccctgc tggccccggt
     1861 ggcgctgatg ctgctgcgcg acgtgctggt caacgccggc ctgatcggcg gagcgctgac
     1921 gctgccgttc ttcgtcatcg ccgcggccat cgccggcggc ctcggcggtt tctgggccgg
     1981 catggcggcg acggccttcg gcggcttcct gctcagtcag tacgtgtatc ccggcgacgg
     2041 ccacgaagca atggcgctgc gcctgctcaa cctgttcctg ctggggctga cggtgagcgt
     2101 ggtggccggc gcccagcagc gcgcaagccg ccgcgcgcag gcgctggccg acaacgcggc
     2161 gcgccgcgcg gccgacctgc aggacatcaa cgaccgcctc agccggcagg agcgctcgct
     2221 gctgcaggcc cagcgcgtgg cgcatttcgg cacctgggaa gtggacgtca agctcggcat
     2281 caaccgcttt tccgacgagc tgtgcgtact catcggcatc gagccgggca cgccgctgga
     2341 cttcgccgcc gccagcgcgc tgacctaccc ggaagacatc gcgcgcgtcg acgccagctt
     2401 ccgcaactgc ctggcgcagc gcgaggcggt gtggcgcgtg gagcaccgcc ggctgcaccc
     2461 gcagcgcggg ctgatctggc tggaagtgcg cgccgaggtg gcctacgacg aacaggggcg
     2521 ggtgacgcgc atgatcggcg ccaccatgga tgtgaccgag cggcgccgcg cggaggacgc
     2581 gctgcgcgcc agcgagcgca tgctgtcgct gatctacgaa aacagccgcg acgcgctgta
     2641 cctggcgcag gtggagtcgg ccgagcgcta cgtgctcgtc tcggccaacc aggctttcct
     2701 caaggtgtcg ggctatcgcc gcgaccaggt gatcggccgg ccgatggaag aggtgatgcc
     2761 ttccctgtcc aaccacgcca aggtgcgtgc gcagtaccag cgcgcgatcg aggcgcggcg
     2821 cgccatcgtc tacgaggaga tcgccgacct gccggccggg cagaagtacg gcgagatcca
     2881 cctgatcccg atcttcaagg aagacgggcc ggtgacgcat atcctgggcg ccatcaagga
     2941 catcaccgac cagcgccgtg cacagaagaa gctgcgcgag agcgaggagc agttccgccg
     3001 cgccatggaa gaggcgccga tcccggtgat catgtgcgcc gacgacggcg aggtgctgca
     3061 ggtgtcgaag acctgggtgc agctcaccgg cttcgcgctg gccgatgtgc ccaccttcga
     3121 gtcatggctg ggcaagtcct acgggca
//
LOCUS       DASZZC010000220         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_659596,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000220 DASZZC010000000
VERSION     DASZZC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659596"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /gene="minC"
                     /locus_tag="VHE37_04760"
     CDS             <1..298
                     /gene="minC"
                     /locus_tag="VHE37_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HVT34866.1"
                     /translation="GQQIYAPDADLILTNTVNPGAEVIADGCVHVYGALRGRAIAGAK
                     GDAQARVFCRRFEAELVAVAGVYAVAEQMQGDLKGKPVQAYLADGKLKIERLEW"
     gene            323..1132
                     /gene="minD"
                     /locus_tag="VHE37_04765"
     CDS             323..1132
                     /gene="minD"
                     /locus_tag="VHE37_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HVT34867.1"
                     /translation="MAKIVVVTSGKGGVGKTTTSASFATGLAQRGKKTVVIDFDVGLR
                     NLDLIMGCERRVVFDFVNVIHGEANLRQALIKDKANENLSILAASQTRDKDALTQEGV
                     EKVLNDLSNEFDYIVCDSPAGIERGAHLAMYFADEALVVTNPEVSSVRDSDRVLGLLS
                     SKTRLVEKGNGRVKEHLVLTRYNPARVEKGEMLSVDDVREILAIPLLGVIPESPAVLT
                     SSNAGTPVIANQDSDAGKAYDDVVARFLGETREHRFITAEKKGLFGRLFGT"
     gene            1145..1405
                     /gene="minE"
                     /locus_tag="VHE37_04770"
     CDS             1145..1405
                     /gene="minE"
                     /locus_tag="VHE37_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HVT34868.1"
                     /translation="MDWLKFFRNEPKNTASLAKERLKVVVAHQRQGRTDAPAYLQRMR
                     EEILEVVRKYVQVPDAAVNVNVQREAGLEVLEMNITLPQQAG"
     gene            complement(1468..2205)
                     /locus_tag="VHE37_04775"
     CDS             complement(1468..2205)
                     /locus_tag="VHE37_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34869.1"
                     /translation="MHAAFLPRPRAMLAAGALAIAATAAHAEPADKVYQPIVEYREFE
                     VELRGGYVHDRDPAVNTAQQYVMDLGYGMTPWWFSEVVMNFKNDPDSSAHVDALELEN
                     IFQLTEQGKYWADLGLFTEYEHPKAKGDPDEIVAGPMLQKEIGRTVNTLDLLASRQIG
                     AGRDPKTHGAYRFQSQWRTGRVVDVGVQAFGDLTGDEKSQMLGPAIFADTHIGDTHIK
                     FDAGVPIGLTHDSPDLLVRWQLELEFH"
     gene            complement(2252..2584)
                     /locus_tag="VHE37_04780"
     CDS             complement(2252..2584)
                     /locus_tag="VHE37_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVT34870.1"
                     /translation="MNLLSRLTCGAVIAACASLAFGADAEFTLTIRDHKFEPAELTVP
                     AGQKIKLTVKNEDATAEEFESHELNREKVIVGKSSGTVYVGPLSAGSYPFFGDFNQDT
                     AQGKLIAK"
     gene            2680..3831
                     /locus_tag="VHE37_04785"
     CDS             2680..3831
                     /locus_tag="VHE37_04785"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HVT34871.1"
                     /translation="MFLFLLPAAMAAAVLVTHAAARDELSPAARLGQKLFNDVSLSAS
                     GAQSCATCHVPAHAHAGSDGRAVPLGGPAMGTQGFRNAPSLRYLSGNTKFLFAADGTP
                     TGGFAWDGRAASLGEQARTPLLAAHEMDNVDAAHFAQKLRRAAYVDEFRQLYGAPVLD
                     DDESVFEHAADALGRFEIEAREFHPYDSKYDAVLAGRARLTAQEARGRQLFDDPQKGN
                     CASCHPSKTVDGQPPLFTDFTYDALGVPRNAEIPATADSGYFDLGLCGPDRKDLAARR
                     ELCGKFKVPTLRNVALTAPYFHNGYFKTLEDAVAFYARRDTSPEQWYPRDAQGRVQKF
                     NDLPPELRGNVNVDEVPYNRKAGDAPALSPQDIDDIVTFLQTLTDGWTP"
     gene            complement(3828..>4342)
                     /locus_tag="VHE37_04790"
     CDS             complement(3828..>4342)
                     /locus_tag="VHE37_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34872.1"
                     /translation="EGKKGAYGAWKALGIPVYQITIQGSTHFEWSLLPTFPATSWCAS
                     TAHGVCEGGWGNPLAQFYTVAWFDRWLKNPGEKGYDDADARLLDDYDWTPRYSFYYRS
                     ARAYAQRDGTPVACEDIRAGCSDTSTPSGTSIPKNTPIAPASSGGRYGGALGLALLLP
                     LLLGARRRRR"
BASE COUNT          735 a         1396 c         1503 g          708 t
ORIGIN      
        1 cggacagcag atctacgcgc cggacgccga cctgatcctg accaacacgg tcaacccggg
       61 agcggaagtg atcgccgacg gctgcgtgca tgtgtacggc gcgctgcgcg gccgcgccat
      121 cgccggcgcc aagggcgacg cgcaggcgcg cgtgttctgc cggcgtttcg aggcggaact
      181 ggtggcggtg gccggtgtgt acgcggtggc ggaacagatg cagggcgatc tcaagggcaa
      241 gccggtgcag gcctacctgg ccgacggcaa gctgaagatc gagcggctcg agtggtaaca
      301 acccaggaca cgtagggacg atatggcaaa gatcgtggtg gtcacttccg gcaagggcgg
      361 cgtgggcaag accacgacca gcgcatcgtt cgccaccggg ctcgcgcagc gcggcaagaa
      421 aaccgtggtg atcgatttcg acgtgggcct gcgcaacctc gacctgatca tgggctgcga
      481 gcggcgcgtg gtgttcgact tcgtcaacgt gatccacggc gaggccaacc tgcgacaggc
      541 gctgatcaag gacaaggcca acgagaacct gtcgatcctc gccgccagcc agacgcgcga
      601 caaggatgcg ctgacccagg aaggcgtgga gaaggtgctc aacgacctgt cgaacgagtt
      661 cgactacatc gtgtgcgatt cgccggccgg catcgagcgt ggcgcgcacc tggccatgta
      721 cttcgccgac gaggcgctgg tggtgaccaa cccggaagtc tcctcggtgc gcgactccga
      781 ccgcgtgctc gggctgctgt cgagcaagac gcggctggtg gaaaaaggca acggccgggt
      841 caaggagcac ctggtgctca cgcgctacaa cccggcacgg gtggagaagg gcgagatgct
      901 cagcgtggac gacgtcaggg agatcctggc cattccgctg ctgggcgtga tcccggaatc
      961 gccggcggtg ctcacctcgt cgaatgccgg cacgccggtc atcgccaacc aggacagcga
     1021 tgccggcaag gcctacgacg acgtggtggc gcgcttcctc ggcgagacgc gcgagcaccg
     1081 cttcatcacc gcggaaaaga agggcctgtt tggccggctg ttcgggacgt aagggggtac
     1141 cgtgatggac tggctcaaat tcttccgcaa cgaaccgaag aacaccgcgt cgctggccaa
     1201 ggagcggctc aaagtggtgg tggcgcacca gcgccagggc cgcaccgacg cgcccgccta
     1261 cctgcagcgc atgcgcgagg aaatcctcga agtggtgcgc aagtacgtgc aggtgcccga
     1321 cgccgcggtc aacgtcaacg tgcagcgcga ggcgggactc gaagtactgg agatgaacat
     1381 cacgctgccg cagcaggccg gctgatccgg ctgcagatga aaaaagcccg cgtcgatcga
     1441 cgcgggcttt ttgtttgcgc gctgcgctca gtggaactcg agttccagct gccagcgcac
     1501 cagcaggtcg ggcgagtcgt gggtcagtcc gatcggcacg ccggcgtcga acttgatgtg
     1561 cgtgtcgccg atgtgggtgt cggcgaagat ggccgggccg agcatctgcg atttctcgtc
     1621 gccggtcagg tcgccgaagg cctgcacgcc gacgtccacc acgcgcccgg tgcgccattg
     1681 cgattggaag cggtacgcgc catgggtctt gggatcgcgg ccggcgccga tctggcgcga
     1741 agccagcagg tcgagcgtgt tgacggtgcg gccgatctcc ttctgcagca tggggccggc
     1801 gacgatttcg tcgggatcac ccttggcctt gggatgctcg tactcggtga acaggccgag
     1861 gtccgcccag tacttgccct gttcggtgag ctggaagatg ttctccagtt ccagcgcatc
     1921 gacatgcgcg ctgctgtcgg gatcattctt gaaattcatc accacctcgc tgaaccacca
     1981 cggggtcatg ccgtagccga ggtccatcac gtattgctgc gccgtgttca ccgccggatc
     2041 gcggtcgtgc acgtagccgc cgcgcagttc cacttcgaac tcgcggtact ccacgatggg
     2101 ctggtagacc ttgtcggcag gttcggcatg cgcggcggtg gcagcaatgg ccagtgcgcc
     2161 ggcggcgagc atggcccgcg ggcgcggcag aaatgcggcg tgcatcggtg agactccctg
     2221 gtggaattgt tatgaaagca gtcgcggcgc cttacttggc gatcagcttg ccctgtgcgg
     2281 tgtcctggtt gaaatcgccg aagaacggat agctgccggc ggacagcggg cctacgtaca
     2341 ccgtgccgct gctcttgccg acgatgacct tttcgcggtt gagctcgtgg ctctcgaatt
     2401 cctcggcggt ggcgtcctcg ttcttcaccg tgagcttgat cttctgcccg gcgggcacgg
     2461 tcagttccgc cggctcgaac ttgtggtcgc ggatggtcag ggtgaactcg gcatcggcgc
     2521 caaaggcgag ggaagcgcag gcggcgatga cggcgccgca ggtgaggcgg gacagcaggt
     2581 tcatggacag gccctggaaa aatcttgagc agtaaatgat aatgattcgc atcagcgttg
     2641 acaagccgtc aggcagcggg acccatgatg cgagcgcggg tgtttctgtt cttgttgccc
     2701 gctgcaatgg cggcggcggt gctcgtcacc cacgcggccg cgcgcgatga gctgtcgccg
     2761 gccgcgcgcc tgggacaaaa actcttcaac gacgtgtcgc tgtcggcttc gggcgcgcag
     2821 tcctgcgcca cctgccacgt gccggcgcac gcgcacgcag gcagcgacgg ccgcgcggtg
     2881 ccgctgggcg gcccggccat gggcacgcag ggattccgca acgcgccgtc gctgcgctat
     2941 ctctccggca acacgaaatt ccttttcgcc gccgacggca cgcccaccgg cggctttgcc
     3001 tgggacggtc gcgctgccag cctcggcgag caggcgcgca cgccgctgct ggcggcgcac
     3061 gagatggaca acgtcgacgc cgcgcatttc gcgcagaagc tgcgccgcgc ggcctatgtc
     3121 gacgagttcc gccagctgta cggggcgccg gtgctggatg acgacgagtc cgtgttcgag
     3181 cacgcggccg atgccctcgg ccgtttcgag atcgaggcgc gcgagttcca tccttacgac
     3241 agcaagtacg acgccgtgct ggccggccgt gcgcgcctta ccgcgcagga agcgcgtggg
     3301 cgccagctgt ttgacgatcc gcagaaaggc aactgcgcgt cgtgccatcc gagcaagacc
     3361 gtcgatggcc agccgccgct gttcaccgac ttcacctatg acgcgctggg cgtgccgcgc
     3421 aatgcggaga ttccggcgac ggccgattca ggctacttcg acctcgggct gtgcggaccg
     3481 gaccgcaagg acctggcggc gcggcgcgag ctgtgcggca agttcaaggt gccgacgctg
     3541 cgcaacgtcg cgctcaccgc gccgtacttc cacaacggct acttcaagac tctggaggat
     3601 gcggtggcgt tctacgcgcg ccgcgatacc agcccggagc agtggtatcc gcgcgatgcg
     3661 cagggccgcg tgcagaaatt caacgacctg ccgccggaac tgcgcggcaa cgtcaacgtc
     3721 gatgaagtgc cgtacaaccg caaggccggc gacgcgcccg cgctgtcgcc gcaggacatc
     3781 gacgacatcg tcaccttcct gcagacactg accgacggct ggacgcccta gcggcgccgg
     3841 cgtcgcgcgc cgagcagcag cggcagcagc agcgccaggc cgagcgcacc accgtagcgg
     3901 ccgccgctgc tggccggcgc gatgggcgtg ttcttcggta tcgaggtgcc ggaaggcgtc
     3961 gacgtgtcgc tgcagcccgc gcggatgtcc tcgcacgcca ccggcgtgcc gtcgcgctgc
     4021 gcgtaggcgc gcgcgctgcg gtagtagaag ctgtagcgcg gcgtccagtc gtagtcgtcg
     4081 agcaggcgcg cgtcggcgtc gtcgtagcct ttctcgccgg gattcttcag ccagcggtcg
     4141 aaccacgcca ccgtgtagaa ctgcgccagc ggattgcccc agccgccctc gcacacgcca
     4201 tgcgcggtgc tcgcgcacca gctggtcgcc gggaacgtgg gcagcaggct ccactcgaaa
     4261 tgcgtgctgc cctggatggt gatctgatag accgggatgc ccagcgcctt ccacgcgccg
     4321 tacgcgcctt tcttgccttc ga
//
LOCUS       DASZZC010000221         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_532026,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000221 DASZZC010000000
VERSION     DASZZC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532026"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VHE37_04795"
     CDS             <1..366
                     /locus_tag="VHE37_04795"
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HVT34873.1"
                     /translation="AQGGIDATVNRCTSNAWLGRDGGWLFDPGLLDVAPQLAIVWARI
                     NQNKTALPARFGRVRRFAGGTAGALQLSLRVRAETTDSSLSYDAIYRDSRGRVVLMLE
                     DIEGTMSGALNRLAGQQTT"
     gene            370..>2605
                     /locus_tag="VHE37_04800"
     CDS             370..>2605
                     /locus_tag="VHE37_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HVT34874.1"
                     /translation="MREPLDIAIIGMAGVYAGARNVAAFWQNILDHVYAVSEASPEWS
                     RSVLDPEAKAANRVYTAQGGWLHDLAEFDPREFGVMPNAVDGREPDHFLALKNARDAL
                     ADAGYLDKPFNHERAGIILGRGNNTNRGAANLLAHGAQLDQMVEIVAKVRPDFSAQDI
                     ERLREGLRAQLPPFNPEMLPGLIPNVTTGVIANRLDLMGPNCIVDAACASSLVALEQA
                     VRELTSGRCDLMLTGGVHAQTPPQSYMIFSQINALARDRIRPFQKGANGTLLGEGCGV
                     VALKRLADAERAGDRIYAVIKGIGVASDGKAKGLFAPRKEGQVVAMRRAYDSCGIEPQ
                     TLGLVEAHATGIALGDRTEVSSLREIFGARRNGVPTIALGSVKSMISHAIPASGAASL
                     IKCALALQQKVLPPMLCEEPAPELELESSPFYINNQTRPWVHGGKAPRRAGVNAFGFG
                     GINAHVILEEHRPRERIVQVQVLHAPSSSELVLLGAADAATLLAQVRQLRGRLDQPGV
                     PGLAAVAKACAARAAGEHRLGVVVQDLAELKTRLDQAVEKLARSDVKPFKTRSGLHYG
                     VGALPGKLCFLFPGEGAQYPGMLADVCVSYPQVREWFDFIEQTAQEGASASRADVIFP
                     APTTLAKDDLHKLEQRLYEEGDLAAESVFAASMGVFDLMMDLGLKPDAMLGHSTGENT
                     ALIAARVRRYDGVSELSHTIHHLNRIYRELDAKGLIAEGTLLTLGGLRPEQRARLLAG
                     PGRGI"
BASE COUNT          438 a          882 c          883 g          402 t
ORIGIN      
        1 gcacagggcg gcatcgacgc cacggtaaac cgttgcacat ccaatgcctg gctcggccgc
       61 gatggcgggt ggctgttcga tccgggcctg ctcgacgtcg cgccacagct ggccatcgtc
      121 tgggcccgca tcaaccagaa caagactgcg ttgcctgcac gcttcggccg tgtgcggcgc
      181 tttgccggcg gcaccgccgg agcgctgcag ctctcgctgc gcgtgcgtgc ggagactacg
      241 gacagttcac tgtcgtatga cgcgatttac cgcgacagcc gcggccgcgt ggtgctgatg
      301 ctggaagaca tcgagggcac catgagcggc gcgctcaatc gcctggcagg tcaacagacc
      361 acctagcgca tgcgcgagcc gctcgacatt gccatcatcg gcatggccgg cgtttacgcc
      421 ggcgcgcgca atgtcgcggc gttctggcag aacatcctcg accacgtcta tgccgtcagc
      481 gaagccagcc cggaatggtc gcgcagcgtg ctcgacccgg aggcgaaggc ggccaaccgt
      541 gtgtacaccg cgcagggcgg ctggctgcat gacctggccg aattcgatcc gcgcgagttc
      601 ggtgtgatgc ccaacgcggt cgatggccgc gaaccggacc atttcctcgc gctgaagaat
      661 gcgcgcgacg cgctggccga cgccggttac ctggacaagc cattcaacca tgagcgggcc
      721 ggcatcatcc tcgggcgcgg caacaacacc aaccgcggcg ccgccaacct gctggcacat
      781 ggcgcgcagc tggaccagat ggtggagatc gtcgccaagg tgcgtccgga tttttccgcg
      841 caggatatcg agcgcctgcg cgagggcctg cgcgcgcagt tgccgccgtt caatccggaa
      901 atgctgccgg gcctgatccc caacgtcacc accggcgtca tcgccaaccg ccttgacctg
      961 atgggaccca actgcatcgt cgatgcagcg tgcgcatcat cgctggtggc gcttgagcag
     1021 gcggtgcgcg aactcacctc tggacgctgt gacctgatgc tgacgggcgg cgtgcacgcg
     1081 cagacgccgc cgcagtcata catgattttt tcgcagatca acgcgctggc gcgcgaccgt
     1141 atccggccgt tccagaaagg cgccaacggc accttgctcg gcgaaggctg cggcgtggtg
     1201 gcactcaagc gcctggccga tgcagagcgt gccggcgacc gcatctacgc ggtgatcaag
     1261 gggataggcg tcgcctccga cggcaaggcc aagggcctgt tcgcgccacg caaggaaggc
     1321 caggtggtgg ccatgcgccg cgcctacgac agctgcggca tcgagccgca gacgctcggc
     1381 ctggtcgagg cgcacgcaac gggtattgcg ctgggcgacc gcaccgaggt cagctcactc
     1441 agggaaattt tcggcgcgcg gcgcaacggc gtaccgacca tcgcgctggg ttcggtcaag
     1501 agcatgatca gccatgccat cccggcgtcg ggcgcggcca gcctgatcaa gtgcgcgctg
     1561 gcgctgcagc agaaggtgct gccgccgatg ctgtgcgagg aaccggcgcc ggaactcgaa
     1621 ctcgaaagct cgccgttcta catcaacaac cagacgcgcc cgtgggtaca tggcggcaag
     1681 gcaccgcgcc gagccggcgt caatgccttt ggtttcggcg gcatcaacgc ccacgtgatc
     1741 ctggaggagc atcgcccgcg cgagcgcatc gtgcaggtgc aggtgctgca tgcgcccagt
     1801 tcctccgagc tggtactgct cggcgcagcg gatgcagcca cgctgctggc gcaggtgcgc
     1861 cagctgcgcg ggcggctcga ccagcccggc gtgccgggcc ttgcggcagt ggccaaggcc
     1921 tgcgccgcgc gcgctgcggg tgaacaccgc ctgggcgtgg tcgtgcagga cctggcggaa
     1981 ctgaagacac ggctggacca ggccgtcgag aagctggcgc gctccgacgt caagccgttc
     2041 aagacgcgca gcgggctgca ctacggcgtg ggcgcgctgc cgggcaagct gtgcttcctg
     2101 ttcccgggtg agggcgcgca atatcccggc atgctggcgg atgtctgcgt gtcctacccg
     2161 caggtgcgcg agtggttcga tttcatcgag cagaccgcgc aggaaggcgc atcggcctcg
     2221 cgcgcggacg tcatcttccc cgctcccacg acgctggcaa aggatgatct gcacaagctt
     2281 gagcagcgcc tctacgagga aggtgatctc gccgcagagt cggtgttcgc cgccagcatg
     2341 ggcgtgttcg acctgatgat ggacctgggc ctcaaacccg acgccatgct cggccattcc
     2401 accggcgaga acaccgcgct gatcgccgcg cgcgtgcgcc gctacgacgg cgtcagcgag
     2461 ctgtcgcaca ccattcatca cctcaaccgc atctaccgcg aactcgacgc caaggggctg
     2521 atcgcggaag gcacgctgct gaccctcggc ggtctgcgcc cggagcagcg cgcgcggctg
     2581 ctggccggcc ccggccgcgg catcg
//
LOCUS       DASZZC010000222         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_20165,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000222 DASZZC010000000
VERSION     DASZZC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20165"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VHE37_04805"
     CDS             <1..350
                     /locus_tag="VHE37_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT34875.1"
                     /translation="VAMAALASVGLADRAGHRPSELSGGQQQRVAIARALAMQPVLVL
                     ADEPSGNLDTHSADGVFDLMRRVNQDSGTTFLIVTHDPRLARRCDRIVELVDGRIASD
                     RPNVPVRISAQAA"
     gene            complement(338..1201)
                     /locus_tag="VHE37_04810"
     CDS             complement(338..1201)
                     /locus_tag="VHE37_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVT34876.1"
                     /translation="MAFDLRPSRQRLAFVLAGGGSCGAAQVGMLRELVRAGIRPDLVV
                     GSSVGALNGAWFAGAPHAAGVAQMEHVWRNLRSRDVFPWNFSSVLRLLRRAHSLVDAA
                     PVRALLARHLPCARLEHCALPAHVVATDAISGEAVRLSHGCTVEAVLASIAIPAVLPP
                     VVRDGRLLIDGGVACNTPVAAAVELGATHVLVLPTAPSYTAQQPPRSALEFGLHAVSL
                     LLARNAQAQLAHHAARVPVLTLPSPDSLPVPGFEFSRSGELIECAAQSTRAWLDAGGL
                     DAFELPALQAA"
     gene            complement(1287..1625)
                     /locus_tag="VHE37_04815"
     CDS             complement(1287..1625)
                     /locus_tag="VHE37_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34877.1"
                     /translation="MKPRVSATDKAVLEIFLRYDVPEMGVLELPLVREQWRALRLRRR
                     DLDDALLALIRRRWLLPLRADGIDYLKLTRRGFRHATVLPVRWSTLLHDAFVLRRHHH
                     AVRAARPATY"
     gene            complement(1622..2392)
                     /locus_tag="VHE37_04820"
     CDS             complement(1622..2392)
                     /locus_tag="VHE37_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HVT34878.1"
                     /translation="MALLLALFAAPVHAWLPPVPAPPPVGQHVVDTTGTLSQAQLAYL
                     EQAIAAHPARGHERLAVLVIHTPFPELIEEYSLRTMQGWGRDAQRPLVLLVIARDTAT
                     AFIEADAGLDRVITDDIGRSIVADHITPPLREHHLYQATRAGVERTLEALEGQPLRAP
                     AWFSLLHWQRSRLFIPSLLLAALAAGALMGCLLGRRGFLLSTVFAAICTWHFSTAGWA
                     TVASAFLAFAACMLSGRRADEAEDKEPDHVWTEQRGRP"
     gene            complement(2455..3021)
                     /locus_tag="VHE37_04825"
     CDS             complement(2455..3021)
                     /locus_tag="VHE37_04825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HVT34879.1"
                     /translation="MPAPIVWLLALNLAGFGVELALGEDAITLGALWPLGAGFAPWQL
                     LTSAFLHAGIAHLATNMFGLWMFGRDVARVLGARWFLALYGASVLSAALAQLAVTSLG
                     DPVPTLGASGGVFGVLGAFAMLFPRRPVALLFPPVVLPSRVFVVVYALIELASGMEGW
                     NAGVAHYAHLGGLAGGWWVMRRWRRAAA"
     gene            complement(3038..3265)
                     /locus_tag="VHE37_04830"
     CDS             complement(3038..3265)
                     /locus_tag="VHE37_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34880.1"
                     /translation="MPAHASGALDDGDLRLLRDLARAEDDAQPAPAAFVDSLVRLSRA
                     GCVRGDAQRTVRVTDRGRQFLRDCASPSTVH"
     gene            complement(3267..3830)
                     /locus_tag="VHE37_04835"
     CDS             complement(3267..3830)
                     /locus_tag="VHE37_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HVT34881.1"
                     /translation="MNLMDAIFRRRATRAYRHFPVGDEVIHALLRAAVMAPSAMNLQP
                     WSFVLVKGTERLRHVSGQARAYMLTNRDAALPASLKATLEDPAFDLFYGAPALLVVCA
                     TSPAQQAAEDCAMAAQNLMLAACEHQLASCCIGLARGWLDSAAGRSALGLPAEWIPIV
                     PIALGYAAEEPPAPARREPLLHVAGDD"
     gene            complement(4014..4328)
                     /locus_tag="VHE37_04840"
     CDS             complement(4014..4328)
                     /locus_tag="VHE37_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34882.1"
                     /translation="MNAHYDTLPPDEWSDPVWLSQHPGASPQQLRRRLYAFPTWADAH
                     KFGDRLALNALGVQYGDYAGDIELPWNVTVLATAEQAQALDGAAATLDRLAMLTGGRR
                     LR"
     gene            complement(4325..>4645)
                     /locus_tag="VHE37_04845"
     CDS             complement(4325..>4645)
                     /locus_tag="VHE37_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT34883.1"
                     /translation="DPSQRSLFMRGLLWAALPCGILYGVLALCALAGDAARGALLAAA
                     FGLGTVPGAALGSGSLGTLLRRAGLPSAAPAGALLLIALGLLQAAVVVLHRSLDPAWC
                     RWLA"
BASE COUNT          758 a         1621 c         1684 g          582 t
ORIGIN      
        1 gggtggcgat ggcggcgctg gccagcgttg ggttggccga ccgggcgggg catcggccaa
       61 gtgagttgtc cggcgggcag cagcagcgcg tggcgattgc gcgcgcgctg gccatgcagc
      121 cggtgctggt gctggccgat gagcccagtg gcaatctcga cacgcattcg gccgacggcg
      181 tgttcgacct gatgcgccgg gtcaaccagg acagcggcac gaccttcctg atcgtcaccc
      241 acgatccgcg gctggcgcgc cgctgcgacc gcattgtcga gctggtggat ggccgcatcg
      301 cctccgaccg gccgaacgtg ccggtgcgga tttccgctca ggcggcctga agcgcgggca
      361 gctcgaaggc gtcgagcccg ccggcatcga gccaggcgcg ggtggactgc gccgcgcact
      421 cgatcagttc gccgctgcgc gagaactcga agcccggcac cggcaggctg tcgggtgacg
      481 gcagcgtgag caccggcacg cgcgcggcgt ggtgcgccag ctgcgcctgc gcgttgcgcg
      541 ccagcagcag cgagacggca tgcaggccga attccagggc ggagcgaggc ggctgctgcg
      601 cggtgtagga aggcgcggtg ggcagcacca gcacgtgcgt ggcgcccagc tccactgccg
      661 cggccaccgg cgtgttgcag gccacgccgc catcgatcag caggcggccg tcgcgcacca
      721 ccggcggcag caccgcgggt atggcgatgc tggccagcac cgcctcgacc gtgcagccgt
      781 gcgacaggcg caccgcttcg ccgctgatgg cgtcggtagc gaccacgtgc gccggcagcg
      841 cgcaatgctc aagccgcgcg cacggcaggt ggcgcgccag cagcgcgcgc accggcgcgg
      901 cgtcgaccag cgagtgcgcg cggcgcagca ggcgcagcac gctggagaaa ttccacggga
      961 agacgtcgcg gctgcgcagg ttgcgccaca cgtgttccat ctgcgccacg cccgcggcgt
     1021 gcggcgcacc ggcgaaccag gcgccattga gcgcgccgac cgaggagccg accaccaggt
     1081 cgggccggat gccggcgcgc accagttcgc gcagcatgcc cacctgcgcg gcgccgcagc
     1141 tgccgccgcc cgcgagcacg aaggccaggc gttgccggga aggacgcaga tcgaaggcca
     1201 tggccgccgc tccgttttga tcgtgagtac agcctgcgcc tgaatcgcct gcggcgaaat
     1261 ccgcggatac cgaaacggat ctgcggctag tacgtagcgg gccgagccgc gcgcacggcg
     1321 tggtgatgac ggcgcagcac gaaggcgtcg tgcagcagcg tgctccagcg caccggcagc
     1381 acggtggcgt ggcggaagcc gcgccgggtg agcttgagat agtcgatgcc atcagcgcgc
     1441 agcggcagca gccagcggcg gcggatcagt gcaagcagcg cgtcgtcgag gtcgcggcgg
     1501 cgcaggcgga gcgcgcgcca ctgctcgcgc accagcggca gttcgagcac gcccatctcc
     1561 ggcacgtcgt agcgcaggaa aatttccagc accgccttgt cggtggcgct gacgcgcggt
     1621 ttcacggccg gccccgctgt tcggtccaca cgtggtcggg ctccttgtct tccgcctcgt
     1681 cggcgcggcg gccgctcagc atgcaggcgg cgaacgcgag gaacgcggag gcgacggtgg
     1741 cccagccggc ggtggagaaa tgccaggtgc agatggcggc aaacacggtg ctgagaagaa
     1801 agccgcgccg cccgagcagg cagcccatca gcgcaccggc cgccagcgcc gccagcagca
     1861 gcgagggaat gaacaggcgc gagcgctgcc agtgcagcag gctgaaccag gcgggcgcac
     1921 gcagcggctg cccttcgagc gcttccagcg tgcgctccac accggcccgt gtggcctggt
     1981 acagatggtg ttcgcgcagc ggcggcgtga tgtgatcggc gacgatgctg cgcccgatgt
     2041 catcggtgat cacgcggtcg agcccggcat cggcctcgat gaacgcggtg gcggtgtcgc
     2101 gcgcaatcac cagcagcacc agcggccgct gcgcatcgcg gccccagccc tgcatggtgc
     2161 gcagcgaata ttcctcgatc agttcgggaa acggcgtgtg gatcaccagc accgcgagcc
     2221 gctcatgccc acgcgccgga tgcgcggcga tggcctgttc caggtacgcc agctgcgcct
     2281 gcgacagtgt gccggtggta tcgacgacgt gctgcccgac cggcggcggt gccggcaccg
     2341 gcggcagcca tgcatgtacc ggcgcggcga acagcgccag cagcagagcc acatgaaata
     2401 ttctcgacat gcacgcagcg tgcgtcacgc acgcacgcgg cgcattgacc caggtcaggc
     2461 ggccgcgcgg cgccagcggc gcatcaccca ccagccgccg gccaggccgc ccaggtgcgc
     2521 gtagtgcgcc acgccggcat tccagccttc catgccggag gccagttcga tcagcgcgta
     2581 gaccacgacg aacacgcgcg acggcagcac gacgggtgga aacagcagcg ccaccggccg
     2641 ccgcgggaac agcatggcga acgcgcccag cacgccgaac acgccgcccg atgcgccgag
     2701 cgtgggcacc gggtcgccca ggctggtcac cgccagctgc gccagcgcag cgctgagcac
     2761 gctggcgccg tacagggcga ggaaccagcg cgcgccgagc acgcgcgcca cgtcgcggcc
     2821 gaacatccac aggccgaaca tgttggtcgc caggtgggcg atgccggcgt gcaggaaggc
     2881 gctggtcagc agctgccacg gcgcgaagcc cgcgcccagc ggccacagcg cgccgagcgt
     2941 gatggcatct tcgcccagcg ccagctcgac accgaagccg gccaggttga gtgcgagcag
     3001 ccagacgatc ggtgccggca tgccagtcgg atcccgctca gtgcacggtg gaaggggacg
     3061 cgcagtctcg cagaaattgc cggccgcgat cggtcacgcg cacggtgcgc tgcgcatcgc
     3121 cgcgcacgca gcctgcgcgc gacagccgca ccaggctgtc gacgaatgct gccggcgccg
     3181 gctgcgcgtc gtcctcggcg cgtgccaggt cgcgcagcag gcgcaggtcg ccgtcgtcga
     3241 gtgcgccgct ggcatgagca ggcatgtcaa tcgtccccgg cgacgtgcag cagcggctcg
     3301 cgccgtgccg gcgccggcgg ttcctccgcc gcgtagccca aagcgatcgg cacgatgggt
     3361 atccactccg caggcaatcc cagcgcgctg cggccggcgg cggaatcgag ccagccgcgc
     3421 gccagcccga tgcagcagct ggccagctgg tgctcgcacg ccgccagcat caggttctgt
     3481 gcggccatgg cgcagtcctc cgcggcctgc tgcgcgggcg aggtggcgca caccaccagc
     3541 agcgccggcg cgccgtagaa caggtcgaac gcgggatctt ccagcgtggc cttcagcgag
     3601 gccggcagcg cggcgtcgcg gttggtcagc atgtaggccc ttgcctgccc ggaaacgtgg
     3661 cgcagccgct cggtaccttt caccagcacg aagctccagg gctgcaggtt catcgcgctc
     3721 ggcgccatca ccgcggcgcg cagcagggcg tggatcacct cgtcgccgac cggaaagtgc
     3781 cggtacgcgc gcgtggcgcg gcggcggaag atggcatcca tcaggttcat gcgggcggct
     3841 ccacggaaat ctgcctgcag catagaggcg cagtgcgcgc ggctcattga cgtgcatcaa
     3901 tggctttttt tgtatgaccg cactgccctt ggcgatgtta taaaatattt tataaaatta
     3961 taaatataca attattaata acagcatgac cggccatgcg gtcaggcaaa aaatcagcgc
     4021 aggcgccgtc cgccggtcag catcgccagc cggtcgagcg tggctgcggc gccatccagt
     4081 gcctgcgcct gttcggccgt ggcgagcacg gtcacgttcc agggcagctc gatgtcgccg
     4141 gcgtagtcgc cgtactgcac gcccagtgca ttgagcgcga gccggtcgcc gaacttgtgt
     4201 gcgtcggccc aggtgggaaa ggcgtagagc cggcgccgca gctgctgcgg gctggcaccg
     4261 ggatgctggc tcaaccacac cgggtcgctc cactcgtccg gcggcagcgt gtcgtagtgg
     4321 gcgttcatgc gagccagcgg caccacgcgg gatcgagcga gcggtgcagc accaccaccg
     4381 cggcctgcag cagccccagc gcgatgagca gcagcgcgcc ggccggcgcg gcagacggca
     4441 ggccggcgcg gcgcagcagc gtacccaggc tgccgctgcc cagcgcagcg cccggcacgg
     4501 tgcccaggcc gaaggcggcc gcaagcagcg cgccgcgtgc ggcatcgccg gccagcgcgc
     4561 acagcgcgag cacgccgtag aggattccgc agggcagcgc ggcccacagc aggccgcgca
     4621 taaacagcga gcgttgcgat gggtc
//
LOCUS       DASZZC010000223         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_468914,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000223 DASZZC010000000
VERSION     DASZZC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468914"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /gene="tatC"
                     /locus_tag="VHE37_04850"
     CDS             complement(<1..572)
                     /gene="tatC"
                     /locus_tag="VHE37_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HVT34884.1"
                     /translation="MSDTPDAGAEQPLIVHLLELRARLLSAIIGFVIVLLPLAYFAKD
                     IYGVVAAPLLKLMPPGSSMIATEVASPFVAPLKLAAIIAAAISMPWTLWQVWSFVAPG
                     LYKSEKRLAAPLMVSSTLLFYGGMAFAYFLVLPAVFRFLLAVAPAGVAVMTDINHYLD
                     FVLGLFLAFGLSFETPVAIVLIVATGFVTPG"
     gene            complement(580..873)
                     /gene="tatB"
                     /locus_tag="VHE37_04855"
     CDS             complement(580..873)
                     /gene="tatB"
                     /locus_tag="VHE37_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HVT34885.1"
                     /translation="MFDVGFSELVLCFVVALVVLGPERLPAVARAFGRWTGMARVYLR
                     NLSAELERETQAAELKKQLLEARQSIEQHAAGLQQSVKDEAGKLAHDADPRKP"
     gene            complement(881..1090)
                     /gene="tatA"
                     /locus_tag="VHE37_04860"
     CDS             complement(881..1090)
                     /gene="tatA"
                     /locus_tag="VHE37_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HVT34886.1"
                     /translation="MGSMSIWHWLLVLVIVVLIFGTKKLRNAGSDLGGAVKNFKDAMR
                     EGEAEAGRKDALPPNGSGKPDDGKN"
     gene            complement(1159..>1865)
                     /locus_tag="VHE37_04865"
     CDS             complement(1159..>1865)
                     /locus_tag="VHE37_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34887.1"
                     /translation="WATLAPLLWTLAALGLKALCQQPLTRRRVLSLAAALLLQLLLWL
                     HAGFAPWSRTLVLSMAGAGALAGLVAVLARSARWQLPAAVGLLLYAASGPAWYHAYDF
                     APMFTVDDESFVKQGLFLKQVLPPDTRVATAWIGAPAYFSDLYFIDIEGKVDPHVAHI
                     QGSEPFRAGFTKRDPAWSVGRLKPDVLLYRSPQWGCYGYTPLSNDLCVRPDRLPALAA
                     LAGNWCDEHDRSRYCP"
BASE COUNT          330 a          645 c          635 g          255 t
ORIGIN      
        1 cccggcgtga cgaaaccggt ggcgacgatc agcacgatgg ccaccggcgt ctcgaacgac
       61 aggccgaagg cgagaaacag gccgagcacg aaatcgaggt agtggttgat gtcggtcatc
      121 accgccacgc cggccggcgc caccgccagc aggaagcgga acaccgccgg cagcaccagg
      181 aaatacgcga aagccatgcc gccgtagaac agcagcgtgc tggagaccat cagtggcgcg
      241 gccaggcgct tctcgctctt gtacaggccg ggggcgacga agctccacac ctgccacagc
      301 gtccacggca tggagatggc cgcggcgatg atcgcagcca gcttgagcgg cgccacgaac
      361 ggcgaggcca cctcggtggc gatcatcgag ctgcccggcg gcatcagctt gagcagcggc
      421 gcggccacca cgccgtagat gtccttggcg aaatatgcca gcggcagcag cacgatgacg
      481 aagccgatga tcgcggacag cagccgcgcg cgcagttcca gcaggtggac gatcagcggc
      541 tgctcggcgc cggcgtcggg cgtatcgctc acgatgagct cacggcttgc gcggatcggc
      601 gtcgtgcgcg agcttgccgg cctcatcctt caccgactgc tgcaggccgg ccgcgtgctg
      661 ctcgatcgac tgcctggctt cgagcagctg cttcttcagt tcggccgcct gcgtctcgcg
      721 ctcgagctcg gccgacaggt tgcgcaggta cacgcgcgcc atgccggtcc agcggccgaa
      781 ggcacgcgcc accgccggca ggcgctcggg gccgagcacc accagtgcca cgacgaagca
      841 cagcaccagc tcggaaaagc cgacgtcgaa cacgcgtcgc tcagttcttg ccgtcgtcgg
      901 gcttgccgct gccgttgggc ggcagcgcgt ccttcctgcc ggcctcggct tcgccttcgc
      961 gcatggcgtc cttgaaattc ttcaccgcgc cgccgaggtc gctgccggcg ttgcgcagct
     1021 tcttggtgcc gaagatcagc acgacgatga ccagcaccaa cagccagtgc cagatgctca
     1081 tggaacccat atgcagctcc tgcgtggcgg caggcgccgc cggtggaatc cgaagatcat
     1141 gcgatcagcc gatcagggtc acggacaata ccgtgaacgg tcgtgttcgt cacaccagtt
     1201 gccagccagc gcggccagcg cgggcaggcg gtcggggcgc acgcacaggt cgttggacag
     1261 cggcgtgtag ccatagcatc cccactgcgg gctgcggtag agcagtacgt cgggcttgag
     1321 ccggccgacc gaccaggccg ggtcgcgctt ggtgaagccg gcgcggaacg gctcgctgcc
     1381 ctggatgtgc gccacgtgcg ggtccacctt gccttcgatg tcgatgaagt acaggtcgga
     1441 aaaataggcc ggcgcgccga tccaggcggt ggccacgcgc gtgtccggcg gcagcacctg
     1501 tttcaggaac agcccctgct tgacgaagga ttcgtcgtcc accgtgaaca tcggcgcaaa
     1561 atcgtaggcg tggtaccagg ccgggcccga ggccgcgtac agcagcagcc cgactgccgc
     1621 cggcagctgc cagcgcgcgc tgcgggccaa taccgcgacg agcccggcca gcgcgccggc
     1681 cccggccatg ctcagcacca gcgtgcgcga ccatggcgca aagccggcgt gcagccacag
     1741 cagcagctgc agcagcagcg cggcggcgag cgacagcacg cgccttcgcg tgagcggctg
     1801 ctggcacagc gctttcagcc cgagcgccgc cagcgtccac agcagcggcg ccagcgtggc
     1861 ccagt
//
LOCUS       DASZZC010000224         5790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_468924,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000224 DASZZC010000000
VERSION     DASZZC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468924"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="rfbG"
                     /locus_tag="VHE37_04870"
     CDS             complement(<1..813)
                     /gene="rfbG"
                     /locus_tag="VHE37_04870"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HVT34888.1"
                     /translation="MIDAAFWRGRRVLLTGHTGFKGSWLSLWLQRLGAEVTGFALDPP
                     TQPNLFQLAGVGQGMDSIIGDVRDASAVNAALARSRAEVVLHLAAQPLVHAGYSDPAG
                     TYAINVMGTVHVLDAVRTVGGVKAVVNVTTDKCYDNREWVWSYRENDRLGGRDPYSNS
                     KACSELVTASFRDSFFPPEHYAGHGVALGSGRAGNVIGGGDWGADRLVPDFFRAIDAG
                     QPARVRRPQAIRPWQHVLEPLAGYLLLAQKLHQHGCEYAEGWNFAPHEDDAQP"
     gene            complement(810..1577)
                     /gene="rfbF"
                     /locus_tag="VHE37_04875"
     CDS             complement(810..1577)
                     /gene="rfbF"
                     /locus_tag="VHE37_04875"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HVT34889.1"
                     /translation="MKAVILAGGLGTRISEETSVRPKPMVEIGGRPILWHILKIYSAH
                     GINDFIICLGYKGYMVKEYFANYFLHMSDVTFDMASNAMHVHQKNAEPWRVTLVDTGE
                     QTMTGGRLRRVRDYLGDQDFCMTYGDGVGNVDLTGLIAFHRAAGRLATLTATQPPGRF
                     GALDFSGDLIRSFKEKPQGDSSWINGGFFVLSPRVIDYIAGDDTVWEREPMERLAHEG
                     QLAAYRHQGFWQPMDTLRDKLQLEALWQGGKAPWKTW"
     gene            complement(1623..2600)
                     /locus_tag="VHE37_04880"
     CDS             complement(1623..2600)
                     /locus_tag="VHE37_04880"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVT34890.1"
                     /translation="MRALICGVSGQDGAYLSRLLLDKGYTVYGSSRDASSASLTNLYR
                     LGIRDRVQVESMSVMDFRSVLQVLQKVRPDEVYNLAGQSSVSLSFEQPVETFESISVG
                     TLNLLEAIRFSNNAIRFYNAGSGECYGDTAGMAADEDTLFRPASPYAVAKSAAHWAVA
                     TYRQAYKLYACSGVLFNHESPLRPRRFVTRKIVDAACRIAAGEKLRLRLGNTQIVRDW
                     GWAPEYVEAMWLLLQQPQPQDFLIATGASHSLEEFVQLAFAEVGLDWRQHVDSDPSLL
                     RPSDHLRAYANPAKAQRLLGWQARYRMPDVVRMMVAAERDSAMPAGAAG"
     gene            complement(2643..3596)
                     /locus_tag="VHE37_04885"
     CDS             complement(2643..3596)
                     /locus_tag="VHE37_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HVT34891.1"
                     /translation="MKTTARIYVAGHRGLAGSAIVRALTTRGHQGIITRTHAELDLTD
                     AAAVRAFMQAEKPEYVFLAAARVGGIQANNNYPAEFIRDNLAIQTNVIHEAWRAGVQH
                     LLFLGSSCIYPRDCPQPMREEYLLTGPLEATNRPYALAKIAGIEQCWSYNRQYGTHYL
                     AAMPTNLYGPNDNYDLNNSHVLPALIRKCHEAKSRGDASMTAWGTGSPRREFLYSDDM
                     AAACVLLMQRSDEVLAQLPASGPPLVNVGCGEDVTIRELMELVARVTGFTGRIEWDTS
                     KPDGTPRKLLDISRLRQMGWQQAMSLTDGIAHAYADFLARR"
     gene            complement(3593..4714)
                     /gene="gmd"
                     /locus_tag="VHE37_04890"
     CDS             complement(3593..4714)
                     /gene="gmd"
                     /locus_tag="VHE37_04890"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVT34892.1"
                     /translation="MKSKSRKVALVTGITGQDGAYLAELLLDKGYEVHGIKRRTSLFN
                     TNRIDHLYQDPHERNRKLILHYGDLTDSSSLIRVIQQVQPDEIYNLAAQSHVAVSFEE
                     PEYTANSDAVGVLRILEAIRILGLSKKTRFYQASTSELYGLVQEIPQKETTPFYPRSP
                     YGVAKLYGYWITVNYREAYGMYACNGILFNHESPVRGETFVTRKITRALARISLGLQD
                     CLFLGNLAALRDWGHAKDYVEMQWLMLQQDKPEDFVIATGVQHSVRDFVDAAAAELGV
                     KLKWSGKGVKERGTVVSAGKTVMKPGQVIVQVDPRYFRPAEVETLLGDASKARKKLGW
                     SPKISFAELVGEMMREDLKLARRDELVKKKGYKAYDFHE"
     gene            4867..5178
                     /locus_tag="VHE37_04895"
     CDS             4867..5178
                     /locus_tag="VHE37_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34893.1"
                     /translation="MKFAPGLALAAMLALNLPAWAVDDPNAVIDDSPSSAAMAVDLLM
                     VRPLSLAATGVGCVLFVLDLPLSIIQGELPREPARKLVIEPARFTFTRRLGSMEYSSP
                     K"
     gene            complement(5360..>5790)
                     /locus_tag="VHE37_04900"
     CDS             complement(5360..>5790)
                     /locus_tag="VHE37_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HVT34894.1"
                     /translation="FGQSSFAIRAPVETAHTADALREITREVREILTTRKPSAAEVSE
                     AKDSIVKSLPSSIETSGQLDSQMSYILTYGLPDDYYDTYVDKLRALDAAQLDAAARKL
                     VTPSALTWVIVGDLSRIERPIRALKLGEVRVIDAEGKPVP"
BASE COUNT          959 a         1843 c         1877 g         1111 t
ORIGIN      
        1 cggctgagcg tcgtcctcgt ggggtgcgaa attccagccc tccgcatatt cgcagccgtg
       61 ctggtgcagc ttctgcgcca gcagcaggta gccggccagc ggttcgagca catgctgcca
      121 tgggcggatg gcctgcggcc ggcggactct cgccggctgg ccggcgtcga tcgcgcggaa
      181 aaaatccggg accaggcggt ccgcgcccca gtcgccgccg ccgatgacgt tgccggcgcg
      241 cccgctcccc agggctacgc cgtgcccggc gtagtgttcg ggaggaaaaa acgaatcacg
      301 aaacgaagcc gtcaccagtt cggaacaggc cttgctgttg ctgtacggat cgcggccgcc
      361 caggcggtca ttttcccggt agctccatac ccattcgcgg ttgtcgtagc acttgtcggt
      421 ggtgacattg accactgcct tgacaccgcc gaccgtgcgc acggcgtcga gcacatgcac
      481 cgtgcccatg acattgattg cgtaagtccc cgcgggatcg ctgtagccgg catgcaccag
      541 cggttgcgcg gccaggtgca acaccacctc ggcgcggctg cgcgccagcg cggcgttcac
      601 cgcggaggca tcgcgcacat cgccgatgat cgaatccatg ccctggccaa ccccagccag
      661 ctggaacagg tttggctgcg tgggcggatc aagggcgaag ccggtgacct cggctcccag
      721 gcgctgcaac cacagcgaca gccatgaacc cttgaagccg gtgtgaccgg tcagcaggac
      781 gcgtcgaccg cgccagaaag ccgcatcgat caccatgttt tccagggcgc cttgccgccc
      841 tgccacagtg cttcgagctg cagcttgtcg cgcaacgtat ccatcggttg ccagaagcct
      901 tgatggcggt aggcggccag ttgaccttcg tgggccaggc gctccatggg ctcgcgctcc
      961 cacaccgtgt cgtcgccggc gatgtagtcg atcaccctcg gcgacaggac aaaaaagccg
     1021 ccgttgatcc aggagctgtc gccctgcggt ttttccttga acgaacgtat caggtcaccg
     1081 gagaaatcca gtgcaccgaa gcgtcccggc ggctgggtgg cggtgagcgt tgcaagtctg
     1141 ccagccgccc ggtgaaaggc gatgagcccc gtgaggtcaa cgttgcccac gccatcgccg
     1201 taggtcatgc agaagtcctg gtcgcccagg tagtcgcgca cgcggcgcag acggccgccg
     1261 gtcatggtct gctcgccggt atcgaccagc gtcacccgcc agggctcggc attcttctgg
     1321 tgcacatgca tggcattgct ggccatgtcg aaggtgacat ccgacatgtg caggaagtag
     1381 ttggcgaaat actccttcac catgtagccc ttgtagccga ggcagatgat gaagtcgttg
     1441 atgccgtggg ccgagtaaat cttgaggata tgccacagga tcggccgccc gccgatctcg
     1501 accatgggtt tgggccggac gctggtttcc tcggaaatgc gcgtgccgag tccgccggcg
     1561 aggatgaccg ctttcacgag agtgcgctcc gttgatccag actgcccttg caggcgccca
     1621 ttttatcccg cggcgcctgc aggcatggcg ctgtcgcgct cggccgcgac catcatgcgc
     1681 accacgtcgg gcatgcgata gcgtgcctgc cagcccagca ggcgctgcgc cttggccggg
     1741 ttggcatagg cgcgcagatg atcggaaggg cgcagcaagg acgggtcgga atcgacgtgc
     1801 tggcgccagt ccaggcctac ctcggcgaac gccagctgga cgaattcctc caggctgtgg
     1861 ctggcgccgg tggcgatcag gaaatcctgc ggctgcggct gttgcagcag cagccacatg
     1921 gcttcgacat attccggcgc ccagccccag tcgcgcacga tctgggtgtt gcccagccgc
     1981 aggcgcagct tttcaccggc ggctatgcga caagcggcat cgacgatctt gcgcgtgacg
     2041 aagcgcctgg gccgcagcgg cgattcgtgg ttgaacaaca cgccactgca ggcatacagc
     2101 ttgtaggcct ggcgataagt cgcgactgcc cagtgcgccg ccgacttggc cacggcgtag
     2161 ggactggccg gccggaacag cgtgtcctca tcggccgcca tgcccgcggt atcgccatag
     2221 cattcacccg agccggcgtt gtagaagcgg atggcattgt tggagaagcg gatcgcttcc
     2281 agcaggttga gcgtgccgac gctgatgctc tcaaaggttt ccaccggctg ttcaaacgag
     2341 aggctcaccg agctctgtcc ggccaggtta tagacctcgt ccgggcgcac tttctgcagc
     2401 acctggagca cgctgcggaa atccatcacc gacatcgact cgacctgcac tcggtcgcgg
     2461 atgcccagcc ggtaaagatt ggtcaatgac gcgctgctgg catcgcgtga actgccgtac
     2521 acggtgtagc ccttgtccag cagcaggcgc gagaggtatg cgccgtcctg gcctgaaacc
     2581 ccgcagatca atgctctcat gttctgcaat ttagtttggc ctgggccatg cttgccagcc
     2641 gttcaccggc gcgcgaggaa atccgcgtac gcgtgggcaa tgccgtcggt cagcgacatg
     2701 gcttgctgcc agcccatctg cctgaggcgc gagatatcca gcagcttgcg cggcgtgcca
     2761 tccggcttgc tggtgtccca ctcgatgcgg ccggtaaagc cggtgacgcg cgcgaccagt
     2821 tccatcagtt cgcggatggt cacgtcctcg ccgcagccga cgttgaccag cggcggcccg
     2881 cttgcgggca actgcgccag cacctcgtca ctgcgctgca tcagcagcac gcaggcggcg
     2941 gccatgtcgt cgctgtagag gaattcgcgg cgcgggctgc cggtgcccca ggccgtcatg
     3001 ctggcatcgc cgcggctctt cgcttcgtgg cacttgcgga tcagcgcggg cagcacgtgc
     3061 gagttgttga ggtcgtagtt gtcgttgggc ccatacaggt tggtcggcat ggccgcgaga
     3121 taatgggtgc cgtactggcg gttgtatgac cagcactgct cgatgccggc gatcttggcc
     3181 agcgcgtagg ggcgattggt cgcctccagc ggcccggtca gcaggtattc ctcgcgcatc
     3241 ggctgcggac agtcgcgcgg gtagatgcac gacgacccga ggaacaagag atgttgcacg
     3301 ccggcgcgcc acgcttcgtg gatcacgttg gtctggatgg ccaggttgtc gcggatgaac
     3361 tcggccgggt agttgttgtt ggcctggatg ccgccgaccc tggctgcggc caggaacacg
     3421 tattcgggtt tctccgcctg catgaacgcg cgcacggcgg cggcgtcggt gaggtctagt
     3481 tccgcgtgcg tgcgcgtgat gatgccctgg tgtccgcgcg tcgtcagcgc acgcacgatg
     3541 gccgatccgg ccaggccgcg gtgaccggcg acatagatgc gggcggtggt cttcattcgt
     3601 ggaagtcgta ggccttgtag cccttcttct tcaccagctc gtcgcggcgc gccagcttga
     3661 gatcttcgcg catcatctcg ccgaccagtt cggcaaagct gatcttcggc gaccagccca
     3721 gcttcttgcg cgccttgctg gcatcgccca gcagcgtctc gacttccgca gggcggaaat
     3781 agcgcgggtc gacctgcacg atcacctgtc ccggcttcat cacggtcttg ccggcagaca
     3841 ccacggtgcc gcgctccttc acgcccttgc cgctccactt gagcttcacg cccagctcgg
     3901 cggcggcggc atcgacgaag tcgcgcacgc tgtgctgcac gccggtggca atgacgaaat
     3961 cctcgggctt gtcctgctgc agcatcagcc actgcatctc gacgtagtcc ttggcatggc
     4021 cccagtcgcg cagcgcggcg aggttgccca ggaacaggca gtcctgcagc cccaggctga
     4081 tacgcgccag cgcgcgcgtg atcttgcggg tgacgaaggt ttcgccgcgc accggcgact
     4141 cgtggttgaa caggatgccg ttgcaggcat acatgccgta ggcctcgcgg tagttcaccg
     4201 tgatccagta gccgtacagc ttggccacgc cgtagggcga gcgcgggtag aacggcgtgg
     4261 tttccttctg cggaatttcc tgcaccaggc cgtacagttc cgaggtcgag gcctggtaga
     4321 agcgcgtctt cttcgacagg cccaggatgc ggatggcctc gaggatgcgc agcacgccca
     4381 ccgcgtcgga gttggcagtg tattcgggct cttcgaacga caccgccacg tggctctgcg
     4441 cagccaggtt gtagatttcg tccggctgca cctgctggat cacgcggatc aggctcgacg
     4501 agtcggtgag gtcgccgtag tgcaggatca gcttgcgatt gcgctcgtgc gggtcctggt
     4561 acaggtgatc gatgcggttg gtgttgaaca gcgaggtacg gcgcttgatg ccgtgcacct
     4621 cgtagccctt gtccagcagc agttcggcga ggtaggcgcc gtcctgcccg gtgatgccgg
     4681 tgaccagcgc gacttttctg gatttcgatt tcattcagga cgcggcagtc cgcggtagtg
     4741 gtggaaaaac ggcgcacatt gtaaacagcg gcggcccttg gcgacggcag tcccatcttg
     4801 attgacagtt ttaagttccg cgtctaagct cctgacgtac ctgtaaatct cttcggggag
     4861 atgacgatga aattcgcccc tggccttgcc ctcgccgcca tgctggcgct gaacctgccg
     4921 gcatgggctg tcgatgatcc caacgcggtg atcgacgatt cgccgtcgtc cgccgccatg
     4981 gcggtcgacc tgctgatggt gcgcccgttg tcgctggccg ccaccggtgt cggctgcgtg
     5041 ctgttcgtgc tggacctgcc gctgtcgatc atccagggag aactgccgcg cgaacctgcg
     5101 cgcaagctgg tcatcgaacc ggcgcgtttc accttcacgc gccgcctcgg cagcatggaa
     5161 tattcctcac ccaaataacg ctggcgtttt tcctgtgatg ccgccggcag cgatgccggc
     5221 ggctttttta tgcgcgttgc ctcaaaaaca acttgaccgc acctgcctcg acggtgttat
     5281 taattattta tcaaaactaa aatatataga taaataataa caggcaaaag ggcagtgcgg
     5341 tcaggagaaa aacgtcctct cacggcaccg gcttgccctc ggcgtcgatc acgcgcacct
     5401 cgccgagctt gagcgcgcgg atcggccgct cgatgcgcga gagatcgccg acgatcaccc
     5461 aggtcagcgc cgatggcgtc accagcttcc tcgccgccgc gtcgagttgc gccgcatcca
     5521 gcgcgcgcag cttgtcgacg taggtgtcgt agtaatcgtc cggcaggccg taggtgagga
     5581 tgtagctcat ctggctgtcg agctggccgc tggtctcgat gctgctgggc aggctcttga
     5641 cgatggagtc cttggcttcg ctaacttccg ccgcgctggg cttgcgcgtg gtcaggatct
     5701 cgcgcacctc gcgcgtgatc tcgcgcagcg cgtcggccgt gtgggccgtt tccaccggcg
     5761 cgcggatggc gaagctcgac tggccgaagc
//
LOCUS       DASZZC010000225         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_149000,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000225 DASZZC010000000
VERSION     DASZZC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149000"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1069
                     /locus_tag="VHE37_04905"
     CDS             197..1069
                     /locus_tag="VHE37_04905"
                     /inference="COORDINATES: protein motif:HMM:NF023560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spherulation-specific family 4 protein"
                     /protein_id="HVT34895.1"
                     /translation="MRPAACMLLLAALAPLPARAAGLLVPAYFYPNATGINYWMQLPA
                     AGAVPLTAILNPNNGPGSSTDPQYTAALAQVHAAGGRVTGYVHTQYGARAAATVQGEV
                     DQYAALYNVDGIFVDEMSSQSADVPYYAALYAYIKNRNAAWNVVGNPGTNTVEDYLKP
                     ATRGADTLVIYEDKAAHLAAFAPAAWTANYSADHFAAIIHTQPDANAMRAELGAASLA
                     NVGWVYITDDVLPNPYDTLPSYWNAEIAALAPAAPAANSGGGAWGSFAVPLLLLALRA
                     GQKRFHRTGISSVI"
     gene            1149..1967
                     /locus_tag="VHE37_04910"
     CDS             1149..1967
                     /locus_tag="VHE37_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT34896.1"
                     /translation="MNDDDAGGTLRRAGDISLWTRSQGQGEPLLLISGLGYSSWCWYE
                     LAPALAHTRRVITFDNRGTGLSDKAAGPYSIAMLADDAARVLDAHGIAQADVIGHSMG
                     GYIAQALALNHAQRVRRLVLVGTSAGGVDSVPLPEATRAAWEASAKLPPEQSVRMNMP
                     ASFSPGWTDAHPQRFEEFLRRRLVNPTPPLCWQQQYNACVDFFVQGSPVERIAHDTLV
                     IHGTEDRVVPYANGEILSRRLPHARLETFDGVGHIPYLEQPQRFVDALHTHLDF"
     gene            2011..>3434
                     /locus_tag="VHE37_04915"
     CDS             2011..>3434
                     /locus_tag="VHE37_04915"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HVT34897.1"
                     /translation="MKPQDLADPRRRRLLYAGAGLFGLALPACRLLRGAPANGAPFSL
                     GVASGEPDASGFVIWTRLAPQPLDGGGMPDDDVPVQWIVADDEQLTRVVRSGTEIAHA
                     AWAHSVHVEVAGLQPDRWYWYGFRALNARSTVGRARTLPAAGAPMARLRVALASCQHY
                     ESGYYGAYRHMAEDDLDLVLHVGDYIYETSHFSGVRSHAAPEPMSLTQYRNRHALYKL
                     DADLQRAHARFPWIVTWDDHDVQNDYAGDQSQFDDPPALFLLRRAAAYQAYWEHMPLR
                     LAARPRGPDALMYRRFAIGDLIDLHVTDERQYRSDQPCGVPGLWGGWLADCPERTDAS
                     RTLFGAPQERWLFDGLASGRARWNVIAQQLLVAQFDESAGAGHKWWTDGWDGYPAARM
                     RLLGHLHERGITNTVTLGGDAHCYFASDLKLDFDDPASPTVASDFTGTSITSDPGGSA
                     DYFGAMLHKSPHIKFFDSRYRGYAR"
BASE COUNT          556 a         1183 c         1160 g          535 t
ORIGIN      
        1 agcgcgtagc ccgacgcata gggattgacg aagccggcgg caaaggcgcc gatgatgtcg
       61 ggctggcgca gcagcggcgg cacgcagatg gcgatgaaag cggtgctgaa ggccagcgcg
      121 acggtccaca gcgcagcggt ataggcggtt cgattcatgt gcatctcccg ggtggcttcg
      181 atattctgcc gtgaccatga gaccggctgc atgcatgctg ctgctcgccg cgctggcgcc
      241 cttgccggcg cgcgccgcgg ggctgctggt gccggcctat ttttatccga acgcgaccgg
      301 catcaattac tggatgcagt tgccggccgc cggcgctgtg ccgctcaccg ccatcctcaa
      361 tccgaacaac gggccgggca gcagtaccga tccgcaatac accgccgcgc tggcgcaggt
      421 gcacgcggcg ggcgggcgcg tgaccggtta cgtgcacacg cagtacggcg cgcgcgccgc
      481 ggcgacggtg cagggagaag tcgaccagta cgccgcgctc tacaacgtcg atggcatttt
      541 cgtcgacgag atgtccagcc agtcggccga cgtgccgtac tacgcggcgc tgtacgcgta
      601 catcaagaac cggaacgcgg cgtggaatgt ggtcggcaat ccgggcacca acaccgtcga
      661 ggattacctg aagcccgcca cgcgcggcgc cgacacgctg gtgatctacg aggacaaggc
      721 agcccactta gccgccttcg cgccggctgc atggacagcg aattacagcg ccgaccactt
      781 cgccgccatc atccacacgc agccggatgc gaatgcgatg cgcgcggagc ttggcgcggc
      841 ctcgctggcc aacgtgggct gggtgtacat caccgacgac gtgctgccga acccttacga
      901 cacgctgccg tcgtactgga acgcggagat cgccgcgctg gcgcccgcag cgccggcggc
      961 gaattccggc ggcggcgcgt ggggcagctt tgcggttccg ctgttgctgc tggcgctgcg
     1021 cgcaggccaa aaacgcttcc accgcaccgg catttcgagt gttatttaat atacaatgaa
     1081 aataaaatca ataaataatt taaaacacgt tcctgggcag tgcggtagtc tcgtgcgcgg
     1141 gagaaaacat gaacgatgac gacgcaggcg gcacgctgcg ccgcgccggc gacatctcgc
     1201 tgtggacgcg cagccagggg cagggcgagc cactgctgct gatcagcggg ctgggctatt
     1261 cgtcgtggtg ctggtacgaa ctggcgcccg cgctggcgca cacgcgccgt gtgatcacct
     1321 tcgacaaccg cggcaccggt ctctccgaca aggcggccgg gccgtactcg atcgccatgc
     1381 tcgccgacga tgccgcacgc gtgctcgacg cgcacggcat cgcgcaggcc gacgtcatcg
     1441 gccactcgat gggcggctac atcgcgcagg cgctggcgct caatcacgcg cagcgcgtgc
     1501 gcaggctggt gctggtcggc accagcgccg gcggcgtcga cagcgtgccg ctgccggaag
     1561 ccacgcgcgc cgcgtgggag gcctcggcca aattgccgcc ggagcagtcg gtgcgcatga
     1621 acatgcctgc ctcgttctcg cccggctgga ccgacgcgca cccgcagcgc ttcgaggaat
     1681 tcctgcgccg ccggctggtc aacccgacgc caccgctgtg ctggcagcag cagtacaacg
     1741 cctgcgtgga cttcttcgtg cagggctcgc cggtggagcg cattgcgcac gacacgctgg
     1801 tgatccacgg caccgaggat cgcgtggtgc cgtatgccaa cggcgaaatc ctctcgcgcc
     1861 ggctgccgca tgcgcggttg gagacgttcg acggcgtagg ccacatcccg tacctcgagc
     1921 agccgcagcg cttcgtcgac gcgctgcaca cgcacctgga cttctagcgt gcatcacctc
     1981 gcggcaatgt gacgccgtca gccttcgaac atgaaaccgc aagacctcgc cgatccccgc
     2041 cgccgccgcc tgctttacgc cggcgcggga ttgttcgggc tggcactgcc tgcgtgccgc
     2101 ctgctgcgcg gcgcgccggc gaacggcgcg ccgttctcgc tcggcgtcgc ctccggcgaa
     2161 ccggatgcga gcggcttcgt catctggaca cggctggcgc cgcagccgct cgacggcggc
     2221 ggcatgcccg acgacgacgt gccggtgcag tggatcgtcg ccgacgacga gcagctcacg
     2281 cgcgtggtac gcagcggaac cgagatcgcg cacgcggcct gggcgcactc ggtgcacgtc
     2341 gaagtcgctg gcctgcagcc ggaccgctgg tactggtacg gcttccgcgc gctcaacgcg
     2401 cggagcacgg tcgggcgcgc acgaacgctg cctgcggccg gcgcgccgat ggcgcggctg
     2461 cgcgtcgcgc ttgcgtcgtg ccagcactac gagagcggtt actacggcgc ctatcgccac
     2521 atggcagaag atgatctcga tctcgtgctg catgtgggcg actacatcta tgaaacctcg
     2581 cacttcagcg gcgtgcgcag ccacgctgcg ccggaaccga tgtcgctcac gcagtaccgc
     2641 aaccggcacg cgctgtacaa gctcgacgcc gacctgcagc gtgcgcacgc gcgctttccg
     2701 tggatcgtca cctgggacga tcacgacgtg cagaacgatt acgcgggcga ccagtcgcag
     2761 ttcgacgatc cgcccgcgct gttcctgctg cgccgcgccg ctgcctacca ggcctactgg
     2821 gagcacatgc cgctgcggct ggccgcgcgt ccgcgcgggc ccgacgcgct gatgtaccgg
     2881 cgcttcgcca tcggcgatct catcgacctg cacgtgaccg acgagcgcca gtaccgctcc
     2941 gaccagccgt gcggcgtgcc tgggctctgg ggcggctggc tggccgattg cccggagcgc
     3001 accgatgcgt cgcgcaccct gttcggcgcg ccgcaggagc gctggctgtt cgacgggctg
     3061 gcgtcaggcc gggcgcgctg gaacgtgatc gcgcagcagc tgctggtcgc gcagtttgac
     3121 gagagcgccg gcgccgggca caagtggtgg accgacggat gggacggcta cccggcggcg
     3181 cgcatgcggc tgctcgggca tctgcacgag cgcggcatca ccaacacggt gacgctgggt
     3241 ggcgatgcgc attgctactt cgcctcggat ctcaagctgg acttcgatga tccggcctcg
     3301 ccgacggtgg ccagcgattt caccggcacc tcgatcactt ccgatcccgg cggcagcgcg
     3361 gactacttcg gcgccatgct gcacaagagc ccgcacatca aattcttcga cagccgctac
     3421 cgcggttatg cgcg
//
LOCUS       DASZZC010000226         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_660404,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000226 DASZZC010000000
VERSION     DASZZC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660404"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1402
                     /gene="waaA"
                     /locus_tag="VHE37_04920"
     CDS             152..1402
                     /gene="waaA"
                     /locus_tag="VHE37_04920"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HVT34898.1"
                     /translation="MRLVYSALLYLITPLVLLRLAWRGMALRDYWQRWGERFGHVPRR
                     EQGVAAWVHAVSVGESMAALPLIRALVKRHGAGNVLVTTTTPTGSARIREMLGDEVLH
                     TYAPYDLPHVVARFLARLRPRQVVVMETELWPNLFRALATRNIPLLIANARLSPRSFK
                     GYGRVRGFAQATLSDCSAIAAQSEADAQRFTALGAPRERVSVMGNIKFDLTVPEDQRR
                     RGRELRARLGDTRHVWIAASTHEDEEHAALETHREVLSANPESVLIIVPRHPQRFELV
                     ARLIERSGLSWARRGSADLERKRFSVLLGDSMGEMYLYLAAADVAFVGGSIVPVGGHN
                     VLEPAALGVPVLFGPHMHNFEGARDLLLEAGAARQITPRTLAENVVELFRDAPLRRRM
                     GEAGARAVDANRGALVRLLKLLLG"
     gene            complement(1383..>1650)
                     /locus_tag="VHE37_04925"
     CDS             complement(1383..>1650)
                     /locus_tag="VHE37_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVT34899.1"
                     /translation="GIGAMLVLGGVFTALPLIGFADAVRRIPFSLVGVLQYIAPTLQL
                     LCGVLLYGEPFTHVQLVGFACIWSALALYAIDGLRRVLLSPKAA"
BASE COUNT          255 a          569 c          596 g          230 t
ORIGIN      
        1 agcaggcaca gcgtccacag caggccgatg ccgaaccagg tcggccagtg gcgcgggtgc
       61 agcagtccgg gatgaaacgg cggagcgctg tcgctgctcc ggccggcggg cgtatccatc
      121 ggcacattgt agcgactacc atctgccgcc catgaggctt gtctactccg cgctgctcta
      181 tctgatcacg ccgctggtgc tgctgcgcct ggcctggcgc ggaatggcgc tgcgcgatta
      241 ctggcaacgc tggggcgagc gcttcggcca tgtgccgcgg cgcgagcagg gcgtggccgc
      301 ctgggtgcac gcggtgtcgg tgggcgaatc gatggccgcc ctgccgctga tccgcgcgct
      361 ggtgaagcgc catggcgcgg gcaacgtgct ggtcaccacc acgacaccaa ccggctcggc
      421 gcgcatccgc gaaatgctcg gcgacgaagt gctgcacacc tacgcaccct acgacctgcc
      481 gcacgtggtg gcgcgcttcc ttgcgcgcct gcggccgcgc caggtggtgg tgatggaaac
      541 cgagctgtgg cccaacctgt tccgcgcgct ggcgacgcgc aacattccgc tgctgatcgc
      601 caacgcgcgc ctgtcgccgc gctcgttcaa gggttatggc cgggtgcgcg gcttcgcgca
      661 ggccacgctg tcggattgca gcgccatcgc cgcgcagagc gaggccgacg cgcagcgctt
      721 caccgcactc ggcgcgccgc gcgagcgcgt gagcgtcatg ggcaacatca agttcgacct
      781 caccgtgccg gaagaccagc gccggcgcgg gcgcgagctg cgcgcgcggc tgggcgacac
      841 gcgccacgtg tggatcgccg ccagcacgca cgaagacgag gagcacgccg cgctggaaac
      901 gcaccgcgaa gtgctgtcgg ccaacccgga aagcgtgctg atcatcgtgc cgcgccatcc
      961 gcagcgcttc gaactggtgg cgcggctgat cgagcgcagc ggattgtcat gggcgcggcg
     1021 cggctccgct gatctcgagc gcaagcgctt ctcggtattg ctcggcgaca gcatgggcga
     1081 gatgtacctg tatctggcgg cggccgacgt ggccttcgtc ggcggcagca tcgtgccggt
     1141 gggcgggcac aacgtgctgg agccggcggc gctgggcgtg ccggtgctgt tcggcccgca
     1201 catgcacaac ttcgagggcg cgcgcgacct gctgctggaa gccggcgccg cgcggcagat
     1261 cacgccgcgc acgctggcgg aaaacgtggt cgaactgttc cgcgacgcgc cgctgcgccg
     1321 gcgcatgggc gaagccggcg cgcgcgcggt ggatgccaac cgcggcgcgc tggtgcgatt
     1381 gctcaagctg cttttgggct gagcagcacg cgccgcaggc catcgatggc atagagcgcc
     1441 agcgcgctcc agatgcaggc gaagcccacc agctgcacgt gggtgaacgg ctcgccgtag
     1501 agcagcacgc cgcacagcag ctgcagcgtc ggcgcgatgt actgcagcac gcccaccagc
     1561 gaaaacggga tgcggcgcac cgcgtcggcg aagccgatca gcggcagcgc ggtgaacacg
     1621 ccgccgagca ccagcatcgc gccgatcccc
//
LOCUS       DASZZC010000227         4477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_786289,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000227 DASZZC010000000
VERSION     DASZZC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786289"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1183)
                     /locus_tag="VHE37_04930"
     CDS             complement(248..1183)
                     /locus_tag="VHE37_04930"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT34900.1"
                     /translation="MLSLLLPAPLAGAIAYFLLLGSTILAGGSILPVALIKLLVPIAP
                     FRRACSRLAVWLAGFWPRTNRAIYSLIHPRRWRVEINGDIQRGRNYLLVCNHQSWTDI
                     LVLADVFSDKLPFPRFFLKHELLYVPLIGTGCWAMDFPFMKRHSREAVAANPALKGED
                     LEATRRACEIYKTQPVLVINFLEGTRFSEAKRAARQSPYRHLLRPKSAGLAFTLSAMG
                     EQFAGIIDATIAYQPTTRRNILWSWLCGEQDTLTVHAEVLPVPAALLHGDYDGDAQFR
                     AEFQSWVNALWAHKDAELDTLLQPAQAARRVARQF"
     gene            complement(1202..2263)
                     /gene="hemE"
                     /locus_tag="VHE37_04935"
     CDS             complement(1202..2263)
                     /gene="hemE"
                     /locus_tag="VHE37_04935"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HVT34901.1"
                     /translation="MPSLENDRFLRALKRQPVDRTPVWMMRQAGRYLPEYRATRARAG
                     SFLKLCKTPELACEVTLQPLARFPLDAAILFSDILTVPDAMGLGLKFVEGEGPMFERP
                     LRSAADIARIGVPDPEGELRYVMDAVRTIKHELHNKLPLIGFAGSPWTLATYMVEGEA
                     TREFAVIKPMAYGEPALLSQLLDKLADAVAAYLKAQIDAGADAVMLFDTWGGALSAAA
                     YPQFSLAPMKKIVAALKAHAPHTPVILFTKGGGQWLEAMADAGADALGLDWTTPLADA
                     RRRTGGRVALQGNFDPVALLAPRARIREEVARVLSDYGSGPGHVFNLGHGILPQTLPE
                     HAAEMIAAVAELSPRYHRG"
     gene            complement(2250..2951)
                     /gene="pyrF"
                     /locus_tag="VHE37_04940"
     CDS             complement(2250..2951)
                     /gene="pyrF"
                     /locus_tag="VHE37_04940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HVT34902.1"
                     /translation="MIAPQDRLIVALDVPDASAARLLVTRLSDAASFYKIGLELLMAP
                     GFFDLLDWLKAEQKQVFVDLKFFDIPETVARAVKNLAERGADFCTVHGNQAIMQAAAR
                     AKSGKLKVLAVTALTSLDRGDLDDLGFACDVGDLVLSRARRALEAGCDGVVSSGLEVE
                     RLRREAPAKLICVTPGIRPVDNQSDGDQKRVMTPARAIKAGADYLVVGRPIRDAADPR
                     AMAQAIQKEIADALA"
     gene            complement(2955..3266)
                     /locus_tag="VHE37_04945"
     CDS             complement(2955..3266)
                     /locus_tag="VHE37_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HVT34903.1"
                     /translation="MAQFDLHRSRGALARSTPYLVILQADAAATPASVVVAPMRPISK
                     SMPLVKHLHVEVQLDGTSYLVMPEELISMPRDALGSRVGSLASLRFAFTAALDFLFTG
                     F"
     gene            complement(3271..3519)
                     /locus_tag="VHE37_04950"
     CDS             complement(3271..3519)
                     /locus_tag="VHE37_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HVT34904.1"
                     /translation="MSAVIKSKTPRRRLNLTVRGDYVKQAREFGLNVSQVLEEALEQR
                     LKQEREQRWLEENREALEAHARRIERDGLWHKGLTKLY"
     gene            complement(3584..4219)
                     /locus_tag="VHE37_04955"
     CDS             complement(3584..4219)
                     /locus_tag="VHE37_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HVT34905.1"
                     /translation="MSALLLTAAEARVIAALVEKSIATPQYYPMSVNALMLAANQKTC
                     RAPVMNLSEGDVGAALNHLEELKLVSRDPLSARVTKWRHSFQHQLLLKPETLALLVTL
                     MLRGPQTLLELKANASGIGGPAEAEAATTALQDLSDRAHPLVVLLPRAPGRKEARYAH
                     TLCGEVALEDPGASVAEAPPASVAATGLAALEQRVEALELRLAELERRLPG"
     gene            complement(4216..4383)
                     /locus_tag="VHE37_04960"
     CDS             complement(4216..4383)
                     /locus_tag="VHE37_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34906.1"
                     /translation="MRWLTPLLALHAGTHVWDAAAGREAWSGLVADLPGVFALPALAI
                     WLARPRAGAPA"
BASE COUNT          709 a         1526 c         1532 g          710 t
ORIGIN      
        1 ccagctgcgc gaggaattct tctccaagca ggatcagctg tacggcgaat tcgccgcctg
       61 cccggcgtcg cccggcttcg tcgcgcgtgc cgctgcggaa ccctggcgct gatttctggg
      121 gctgcgggca ctccaccgca cagccatttt cgctgttatc aattattatc tgaaaaataa
      181 ttcagacaat tatttataac acaatatgag gcggtgcggt tgggcttttt tggcgcggag
      241 ggttcgctca gaactgccgc gccacgcgcc gcgcggcctg cgccggctgc agcagcgtgt
      301 cgagctctgc atccttgtgc gcccacagcg cgttgaccca ggactggaat tccgcgcgga
      361 attgcgcgtc gccgtcgtag tcaccgtgca gcagtgcggc cggcaccggc agcacctcgg
      421 cgtgcacggt cagcgtgtcc tgctcgccgc acagccagct ccataaaatg ttgcggcgcg
      481 tggtcggctg gtaggcgatg gtggcgtcga tgatgccggc gaactgctcg cccatcgccg
      541 acagcgtgaa ggccaggccc gccgatttcg gccgcagcag gtgccggtac ggcgactgcc
      601 gcgcagcgcg cttggcctcg gagaaacgcg tgccttcgag gaagttgatc accagcaccg
      661 gctgggtctt gtagatctcg caggcgcggc gcgtggcttc gaggtcttcg cccttgagcg
      721 ccgggttggc ggcgacggct tcgcggctgt ggcgcttcat gaagggaaaa tccatcgccc
      781 agcagccggt gccgatcagc ggcacgtaca gcagttcatg cttgaggaag aagcgcggga
      841 agggcagctt gtcggagaac acatcggcca gcaccaggat gtcggtccat gactggtgat
      901 tgcacaccag caggtaattc ctgccgcgct ggatgtcgcc gttgatctcc acgcgccagc
      961 ggcgcggatg tatcagcgag tagatcgcgc ggttggtgcg cggccagaag ccggccagcc
     1021 acaccgccag ccggctgcag gcgcggcgga acggggcgat cggcaccagc agcttgatca
     1081 gcgccaccgg caggatcgag ccgccggcca ggatggtcga gcccagcagc aggaagtagg
     1141 cgatggcacc tgccagcggc gccggcaaca acagcgacag catcgtgttc ctctccagcc
     1201 ttcagccgcg atggtagcgc ggcgatagtt ccgccacggc cgcgatcatc tccgcggcgt
     1261 gctcgggcaa ggtctgcggc aggatgccgt gccccaggtt gaacacgtgt cccggcccgc
     1321 ttccgtaatc agacaatacg cgcgccactt cctcgcggat gcgcgcacgc ggcgcgagca
     1381 gcgccaccgg gtcgaagttg ccctgcagcg ccacgcgccc gccggtgcgc cggcgcgcgt
     1441 cggccagcgg tgtggtccag tccaggccca gcgcgtcggc gccggcatcg gccatcgcct
     1501 ccagccactg gccgccgccc ttggtgaaca ggatcaccgg cgtgtgcggc gcatgcgcct
     1561 tcagcgccgc gacaatcttc ttcatcggcg ccagcgagaa ctgcggatag gccgcggccg
     1621 acagcgcgcc gccccaggtg tcgaacagca tcaccgcgtc ggcgccggcg tcgatctgtg
     1681 ccttgagata tgccgcgacc gcatcggcca atttgtcgag cagctgcgac agcagtgccg
     1741 gttcaccgta ggccatgggc ttgatcacgg cgaactcgcg cgtggcctcg ccttccacca
     1801 tgtaggtggc cagcgtccac gggctgccgg cgaaaccgat cagcggcagc ttgttgtgca
     1861 actcatgctt gatcgtgcgc accgcgtcca tcacgtagcg cagctcgcct tccgggtcgg
     1921 gcacgccgat gcgcgcgatg tcggccgcgc tgcgcaacgg gcgctcgaac atcggtccct
     1981 cgccctcgac gaacttgagc cccagcccca tggcatcggg aacggtgagg atgtcggaga
     2041 acaggatcgc ggcgtcgagc gggaagcgcg ccagcggctg cagcgtgacc tcgcatgcca
     2101 gctccggcgt cttgcacagc ttgaggaagc tgccggcacg cgcgcgcgtg gcgcggtact
     2161 ccggcaaata gcgcccggcc tggcgcatca tccacaccgg cgtgcggtcg accggctgtc
     2221 ttttcagcgc acggaggaag cggtcgttct caagcgaggg catcggcaat ctctttctgg
     2281 atggcctgcg ccatggcgcg cggatcggcc gcatcgcgga tcgggcggcc gaccaccagg
     2341 tagtcggcgc cggccttgat cgcgcgcgcg ggcgtcatca cgcgcttctg gtcgccgtcg
     2401 ctctggttgt cgaccgggcg gatgccgggc gtgacgcaga tcagcttggc cggcgcttcg
     2461 cggcgcagcc gctccacttc cagccccgac gacaccacgc cgtcgcagcc ggcttcgagc
     2521 gcgcggcgtg cacgcgacag caccaggtcg ccgacgtcgc aggcaaagcc cagatcgtcg
     2581 agatcgccgc ggtccagcga ggtgagcgcg gtgacggcaa gcactttcag cttgccgctc
     2641 ttggccctgg ccgccgcctg catgatggcc tggttgccgt gcacggtgca gaagtcggcg
     2701 ccgcgctcgg ccaggttctt caccgcgcgc gccaccgtct cggggatgtc gaagaacttg
     2761 aggtcgacga acacctgctt ctgctcggcc ttgagccagt cgagcaggtc gaagaagccg
     2821 ggcgccatca gcagctccag gccgatcttg tagaagctgg cggcgtcgga caggcgcgtc
     2881 accagaaggc gggcggcgga ggcatcgggt acgtcgaggg cgacgatcag gcggtcttgt
     2941 ggagcaatca cgattcagaa tccggtgaac aggaaatcaa gggcggcagt gaaggcaaaa
     3001 cgcagggacg caagcgagcc gacccgggag ccgagagcgt cgcgcggcat ggaaatcagc
     3061 tcttccggca tgaccagata ggacgtccca tcgagttgca cctcgacgtg caggtgtttg
     3121 accagcggca tcgatttgct gatcggccgc atcggcgcca ccaccacgct ggccggcgtg
     3181 gccgcagcat cggcctgcag gatgacaagg tacggggtgc tccgggccag ggcgccgcgc
     3241 gagcgatgga ggtcgaattg cgccacgcgt tcaatacagt ttggtcagac ccttgtgcca
     3301 cagtccgtcg cgctcgatac gccgtgcatg cgcttcgagc gcctcacggt tttcctccag
     3361 ccagcgctgc tcgcgctcct gcttgaggcg ctgctccagc gcttcttcca gcacctgcga
     3421 aacgttgagg ccgaactcgc gggcctgctt cacgtagtcg ccgcgcacgg tcaggttgag
     3481 ccggcggcgc ggcgttttgg acttgatcac ggcagacatg ggtgcgctcc tgatgcgcgc
     3541 gaattatgcg cccgcagcat gcgcacgggc aatgacgcag ctgtcagccg ggcaggcggc
     3601 gctcgagctc agccagccgc agctccagcg cttccacccg ctgctccagt gcggccaggc
     3661 ccgtggccgc gacggacgcc ggcggcgctt ccgcgacgga agcgccgggg tcttccagcg
     3721 ccacctcgcc gcacagcgta tgggcgtagc gcgcctcctt gcggcccggc gcgcgcggca
     3781 gcagcaccac cagggggtgt gcacggtcag acaggtcttg cagcgcggtg gtggccgcct
     3841 cggcctccgc cggcccgccg atgccggagg cattggcctt cagctccagc agcgtctgcg
     3901 ggccgcgcag catcagcgtg accagcagcg ccagcgtttc cggcttgagc agcagctggt
     3961 gctggaagct gtggcgccac ttggtcacgc gcgcgctcag cggatcgcgc gacaccagct
     4021 tgagctcttc caggtgattc agcgcagcgc cgacgtcgcc ttccgacagg ttcatcaccg
     4081 gcgcgcggca ggtcttctgg ttggcggcga gcatcagcgc gttgacgctc atcgggtagt
     4141 actgcggcgt ggcgatggat ttctccacca gcgcggcgat cacgcgcgcc tcggccgcgg
     4201 tcagcagcag cgcgctcatg ccggcgctcc cgcgcgcggc cgcgccagcc agatggccag
     4261 agccggcagc gcgaacacgc cgggcaggtc ggcgaccagc ccgctccagg cttcgcgccc
     4321 ggccgccgcg tcccacacat gggtgccggc gtgcagcgcc agcagcgggg tcagccatcg
     4381 catcgcgcag tcctcgcatc gaatcgggcg cgcagcataa ggcagaccgc gaccggcgtc
     4441 cgccgcgggc agttcagtgc gcctcggccg ccgcggc
//
LOCUS       DASZZC010000228         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_596166,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000228 DASZZC010000000
VERSION     DASZZC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596166"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VHE37_04965"
     CDS             <1..1176
                     /locus_tag="VHE37_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT34907.1"
                     /translation="WAAFVRRGESIPQAELDRREATLQFDDAINIQYTSGTTGFPKGA
                     TLSHHNILNNGYFVGLTCRYTAADRVCIPVPFYHCFGMVLGNLACTAHGATMVVPAEV
                     FDPLATLQTVQAERCTSLYGVPTMFIAELEHPEFAKFDLSSLRTGIMAGSPCPVEVMK
                     KVTTLMNMKEVTIGYGMTETSPISTQSAADEPFEQRVSTVGRVHPHVEIKIVNPETGA
                     VVPRGEAGEFCTRGYSVMLGYWNNPEATNKSIDSGRWMHTGDLATMDEQGYVKITGRI
                     KDMIIRGGENIFPREVEEFLYTHPCILDVQVIGVPSEKYGEEVMAWVKLREGRQLRSE
                     EIAAWCKGKIASYKIPKYWKICDGFPMTVTGKIQKFRMREVSIEELDLKKAAAIKTA"
     gene            complement(1295..2056)
                     /locus_tag="VHE37_04970"
     CDS             complement(1295..2056)
                     /locus_tag="VHE37_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT34908.1"
                     /translation="MLLKGKRVLITGGNHGIGRATAIACARHGADVAINYLRDPESAE
                     SVVREIEALGRRALAVQGDVAQAASAPAFVDAAVHALGGVDVFVSNAGICPFHSFLDM
                     PQDVLDRTMQVNLHGAYYMCQAAARQMQKQGKGGALIAISSISALVGGAMQTHYTPTK
                     AGVHSLMQSCAIALGPHGIRCNSVMPGTIATEINREDLSDAGKRAYMEKRIPLGRLGE
                     PDDVAGCVVFLASDLARYVTGASVLVDGGAYVNLQ"
     gene            complement(2166..2945)
                     /locus_tag="VHE37_04975"
     CDS             complement(2166..2945)
                     /locus_tag="VHE37_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVT34909.1"
                     /translation="MGKSTSTKRGDAPSGSQTLLRGLDVLEAVAGGASSLIDLADKLD
                     LTRSTAHRLASALVERRYLSFVPREGYTLGSKLLELGYGARQQTNLTRIAREPLEALS
                     ASSEDTVHLGVLENDRALYLEKIPGRRRVEIGSRVGEQQPVTSTGLGKALILDLPEER
                     WHQIYVKEQGRALGNGEAWKTWLKRMRTYAKQGHAFDLEENEDQIRCVSAPVREAGGR
                     IVAAISLSSAAQYMSDARMNTLASDVKSAANSISRQLGWNG"
BASE COUNT          528 a          996 c          969 g          525 t
ORIGIN      
        1 tgggccgcgt tcgtgcggcg gggcgaatcg attccgcaag ccgaactcga ccgccgcgaa
       61 gccacgctcc agttcgacga cgcgatcaac atccagtaca cctcgggtac gaccggcttt
      121 cccaagggcg cgacgctgtc gcaccacaac atcctcaaca acggctactt cgtcggcctg
      181 acctgccgct ataccgccgc cgaccgcgtg tgcatcccgg tgccgttcta ccactgcttc
      241 ggcatggtgc tgggcaacct cgcctgcacc gcgcacggcg caaccatggt ggtgccggcg
      301 gaagttttcg acccgctggc cacgctgcag acggtgcagg ccgagcgctg cacctcgctc
      361 tacggcgtgc cgaccatgtt catcgccgag ctcgagcatc ccgagtttgc caagttcgac
      421 ctgtcgtcgc ttcgcaccgg catcatggcc ggctcgccgt gcccggtgga ggtgatgaag
      481 aaggtcacca cgctgatgaa catgaaggaa gtgaccatcg gctacggcat gaccgagacc
      541 tcgcccatct ccacgcaaag cgccgccgac gagccgttcg agcagcgcgt gtccaccgtg
      601 ggccgcgtgc atccccacgt ggagatcaag atcgtcaacc cggaaaccgg cgcggtggtg
      661 ccgcgcggcg aggcgggcga attctgcacg cgcggctaca gcgtgatgct gggctactgg
      721 aacaatcccg aggcgacgaa caaatcgatc gactccggcc gctggatgca caccggcgac
      781 ctggcgacca tggacgaaca gggctacgtg aagatcaccg gccgcatcaa ggacatgatc
      841 atccgcggcg gcgagaacat cttcccgcgc gaggtcgagg aattcctcta cacccacccg
      901 tgcatcctcg acgtgcaggt gatcggcgtg ccgagcgaaa aatacggcga ggaagtgatg
      961 gcctgggtca agctgcgcga gggcaggcag ctgcgcagcg aggagatcgc cgcctggtgc
     1021 aagggcaaga tcgccagcta caagattccg aaatactgga agatctgcga cggcttcccg
     1081 atgaccgtca ctggcaagat ccagaaattc cgcatgcgcg aagtgtcgat cgaagagctc
     1141 gatctgaaga aggcggcggc gatcaagacc gcgtgagggc gccagaaagc ctgaaccgca
     1201 ccggcatttc cgatgttatt aattattata cgaaagttaa ataggataat atttaataac
     1261 agccacaagg ccggtgcggt tcagcgattt ttgctcactg caggttgacg taagcgccgc
     1321 cgtcgaccag caccgaggcg ccggtgacgt agcgcgccag gtccgaggcc aggaacacca
     1381 cgcagccggc cacgtcgtcc ggctcgccca gccgcccgag cgggatgcgc ttctccatgt
     1441 acgcgcgctt gcccgcgtcg ctcaggtctt cgcggttgat ctcggtggcg atggtgcccg
     1501 gcatcaccga gttgcagcgg atgccatgcg gccccagcgc aatggcgcag gactgcatca
     1561 gcgaatgcac gccggccttg gtcggcgtgt agtgcgtctg catcgcgccg ccgaccagcg
     1621 cactgatcga gctgatggcg atgagcgcgc cacccttgcc ctgcttctgc atctgccgcg
     1681 ccgcggcctg gcacatgtag taggcgccgt gcaggttcac ctgcatggtg cgatcgagca
     1741 cgtcctgcgg catgtcgagg aacgagtgga acgggcagat gccggcgttg ctgacgaaca
     1801 cgtcgacgcc gccaagcgca tgcaccgcgg catcgacgaa ggccggcgcg ctcgccgcct
     1861 gcgccacgtc gccttgaacc gccagcgcgc ggcgcccgag cgcctcgatc tcgcgcacga
     1921 cgctttccgc actctcgggg tcgcgcaggt aattgatcgc cacgtcggcg ccgtggcgcg
     1981 cgcaggcgat tgcggtggcg cggccgatgc cgtggttgcc gccggtgatg aggacacgtt
     2041 tgcctttcag cagcatggcg atctccagag attgtccgtc atgcccgcgc aggcgggcgt
     2101 ccagttaatg cgctcacatc acggccagac gaactggatt cccgcctgcg cgggcatgac
     2161 agatatcagc cgttccagcc gagctgccgg ctgatggagt tggccgccga cttcacgtcg
     2221 gacgccagcg tgttcatgcg cgcatcgctc atgtattgcg ccgcgctcga caggctgatc
     2281 gccgccacga tgcgcccgcc ggcctcgcgc accggcgccg acacgcagcg gatctggtcc
     2341 tcgttttcct cgaggtcgaa ggcgtggccc tgttttgcgt aggtgcgcat gcgcttgagc
     2401 caggtcttcc acgcctcgcc gttgccgagc gcgcggccct gctccttcac gtagatctgg
     2461 tgccagcgct cctcgggcag gtcgaggatc agcgccttgc caaggccggt ggaggtgacg
     2521 ggctgctgtt cgcccacgcg cgagccgatt tccacgcgcc ggcggccggg aattttttcc
     2581 aggtacagcg cgcggtcgtt ttccagcacg cccaggtgca cggtgtcttc gctggaggcc
     2641 gacagcgctt ccagcggctc gcgcgcgatg cgcgtgagat tggtttgctg gcgcgcgccg
     2701 tagcccagct ccagcagctt gctgccgagc gtgtagccct cacgcggcac gaaggacagg
     2761 tagcgccgct cgaccagtgc cgaggccagc cggtgcgcgg tgctgcgcgt caggtcaagc
     2821 ttgtcggcca ggtcgatcag cgaactggcg ccgccggcca ccgcttccag cacatccagt
     2881 ccgcgcagca gggtctggct gccgctgggg gcgtcgcctc tcttggttga cgtgcttttt
     2941 cccatatctt agaattgtat cccatattgt ggaattggca accacgtctt ttgcagtcat
     3001 tcccgcgaag gcgggaat
//
LOCUS       DASZZC010000229         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_20854,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000229 DASZZC010000000
VERSION     DASZZC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20854"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VHE37_04980"
     CDS             complement(<1..360)
                     /locus_tag="VHE37_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt efflux protein"
                     /protein_id="HVT34910.1"
                     /translation="MNDGKPRSRKKAAAPAEGKSGGVWWRRFAKNLGGAPRTREDLVQ
                     LLREANTNQLLDADALSMFQGILGTAETQVRDVMVPRAQMVSVRRSWALDRILPVVVE
                     SGHSRFPVLSDPKDEVAG"
     gene            complement(357..806)
                     /gene="ybeY"
                     /locus_tag="VHE37_04985"
     CDS             complement(357..806)
                     /gene="ybeY"
                     /locus_tag="VHE37_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVT34911.1"
                     /translation="MSASPSVQVQRCVPPAGVPAGSSLRAWAQAALGRARGELTIRIV
                     DAGESHALNKQYRGKDKPTNVLSFQGDRAAQTLGDLVICAPVVAREAREQGKSPRAHW
                     AHMVVHGCLHLIGYDHERDRDAEKMEALEKKILRKLGYPDPYQGALS"
     gene            complement(803..1810)
                     /locus_tag="VHE37_04990"
     CDS             complement(803..1810)
                     /locus_tag="VHE37_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVT34912.1"
                     /translation="MSAQPKSGQARAELTLEPDEAQRIANVNGPFDAHLRQIELRLGI
                     EIRNRGNRYQLLGARADVARGEAVLSDLFNQSLDGVVTTEQVHLALSEHGEDSAESLA
                     QEEDEAPAGEVVVRTRRGVVRGRSAHQKEYLKNLATFDLNFGIGPAGTGKTYLAVASA
                     VHALERDRVRRIVLVRPAVEAGEKLGFLPGDMAEKINPYLRPLYDALYEMLGFERVAR
                     LTEKNVIEVAPLAFMRGRTLNESFIILDEAQNTTVEQMKMFLTRIGFGSVAVVTGDVT
                     QIDLPRHEKSGLRHAMQVLEGVAGIAFAQFQKEDVVRHPLVQAIVHAYEKHEQAQEPR
                     A"
     gene            complement(1807..3147)
                     /gene="miaB"
                     /locus_tag="VHE37_04995"
     CDS             complement(1807..3147)
                     /gene="miaB"
                     /locus_tag="VHE37_04995"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HVT34913.1"
                     /translation="MSKKLYLITYGCQMNEYDSSKMADVLKASHGYERTHSPDEADLL
                     LLNTCSVREKAQEKVFSELGRWKEWKQAAPGRVIGVGGCVASQEGELITRRAPQVDLV
                     FGPQTLHRLPQLLDSARATQKPAVDISFPEIEKFDRLPAPRAEGPCAFVSIMEGCSKY
                     CSFCVVPYTRGTEMSRPLDDVLAECAALTAQGVREITLLGQNVNAYRGRMGDTAETCD
                     LALLIHYLARDAKLQRIAYTTSHPAEFTDELIEAYAEEPKLSSWLHLPVQSGSDRILS
                     MMKRQYTRAQYLDKIARLRAVRPEISLSSDFIVGFPGETEDDFAQTMSLIDEAGVDTA
                     YSFHYSPRPGTPAANLRDGVSAEEKQRRLSLLQHRVNQLDRAFRDRLVGSTQQVLVEK
                     PAARGGGQVAGRTTTNRTVNFDGDESMIGQLVEVEISEATTYSLRGRLREAVTA"
     gene            complement(3211..3642)
                     /locus_tag="VHE37_05000"
     CDS             complement(3211..3642)
                     /locus_tag="VHE37_05000"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HVT34914.1"
                     /translation="MFNRILIVCTGNICRSPMAEGYMRSRLPAEKSVSSAGIAASPGE
                     PAAPEAVAVMAAHGFDISSHGALQITDGQAHAADLILVADQTHQRWIARRFPQLRGRV
                     HKLLKWEGNRDIEDPYGLPQSYFEQTYAEIEMGVSDWLKRL"
     gene            complement(3679..>3983)
                     /locus_tag="VHE37_05005"
     CDS             complement(3679..>3983)
                     /locus_tag="VHE37_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVT34915.1"
                     /translation="FSAIAWNGRYLVIGFASGTIPQIALNRLLLKQASAVGVFWGAFV
                     AREPKANAENFQQLFAWHAAGKLKPHVSHQYPLAQGAQALRDMMDRKVTGKVVLIP"
BASE COUNT          588 a         1396 c         1301 g          698 t
ORIGIN      
        1 gccggccacc tcgtccttgg gatcggacag caccgggaag cgcgagtggc cgctctccac
       61 caccaccggc aggatcctgt cgagcgccca gctgcgccgc accgacacca tctgcgcgcg
      121 cggcaccatc acgtcgcgca cctgcgtttc cgcggtgccg aggatgccct ggaacatcga
      181 cagcgcatcg gcgtcaagca gctggttggt gttggcttcg cgcagcagct gcaccaggtc
      241 ctcgcgcgtg cgcggcgcgc cgcccaggtt cttggcgaag cgccgccacc agacgccgcc
      301 gctcttgccc tctgccggcg cggcagcctt cttccggctg cggggcttgc cgtcgttcac
      361 gacagcgcgc cctggtacgg atcggggtat cccagcttgc gcaagatctt cttctccagg
      421 gcttccattt tttctgcgtc gcggtcgcgc tcgtgatcgt agccgatcag gtgcaggcag
      481 ccatgcacca ccatgtgcgc ccagtgcgcg cgcgggctct tgccctgctc gcgcgcctcg
      541 cgcgccacca ccggcgcgca gatgacgaga tcaccgagcg tctgcgccgc gcggtcgccc
      601 tggaacgaca gcacgttggt cggcttgtcc ttgccgcggt actgcttgtt cagcgcgtgg
      661 ctctcgccgg catcgacgat gcggatggtg agttcgccgc gcgcgcggcc cagcgccgcc
      721 tgcgcccacg cgcgcaaact tgaacccgct ggcacgccag caggcggcac gcagcgctgc
      781 acctgcacgc tcgggctggc gctcatgcgc gcggctcctg cgcctgctcg tgcttttcgt
      841 acgcatgcac gatggcctgc accagcggat gacgcaccac gtcttccttc tggaactgcg
      901 cgaaagcaat gccggccacg ccctcgagca cctgcatggc atggcgcagc ccgctcttct
      961 cgtggcgcgg caggtcaatc tgcgtcacgt cgccggtcac cactgccacg ctgccaaatc
     1021 ctatccgcgt gaggaacatc ttcatctgct cgaccgtggt gttctgcgcc tcgtcgagaa
     1081 tgatgaacga ctcgttgagc gtgcggccgc gcatgaaggc cagcggcgcg acctcgatga
     1141 cgttcttctc ggtcagccgc gccacgcgct cgaagccgag catctcgtac agcgcgtcgt
     1201 acagcgggcg caggtacggg ttgatcttct ccgccatgtc gccaggcagg aaacccagct
     1261 tctcgcccgc ttccaccgcc ggccgcacca gcacgatgcg gcgcacgcgg tcgcgctcca
     1321 gcgcatgcac cgcgctcgcc accgcaagat aggtcttgcc ggtacccgcc gggccgatgc
     1381 cgaaattgag atcgaaggtg gcgaggttct tcagatactc tttctggtgc gcgctgcgcc
     1441 cgcgcaccac gccgcggcgc gtgcgcacca ccacttcgcc ggccggcgcc tcgtcttctt
     1501 cctgcgccag cgattcggcg ctgtcctcgc cgtgctcgga caaggcgagg tgcacctgct
     1561 cggtggtgac cacgccgtcg agcgactggt tgaacaggtc cgacagcact gcctcgccgc
     1621 gcgccacgtc ggcgcgcgcg ccgagcagct ggtagcggtt gccgcggttg cggatctcga
     1681 tgcccaggcg cagctcgatc tgtcgcaggt gcgcgtcgaa tgggccgttg acgttggcga
     1741 tgcgctgcgc ctcgtcaggc tccagcgtca gctcggcgcg ggcctggccg gacttgggtt
     1801 gcgcgctcat gccgtcaccg cttcgcgcag gcgcccgcgc agcgaatagg tggtcgcctc
     1861 gctgatctcc acttcgacca gttgcccgat catcgattca tcgccgtcga aattcaccgt
     1921 gcggttggtc gtggtgcgcc ctgccacctg gccgccgccg cgcgccgccg gcttttccac
     1981 cagcacctgc tgcgtgctgc caaccagtcg atcgcggaac gcccggtcca gctggttgac
     2041 ccggtgctgc agcagcgaca ggcgacgctg cttttcctcg gcgctgacgc cgtcacgcag
     2101 gttggccgcc ggcgtgccgg ggcgcgggct gtagtggaag ctgtaggcgg tatcgacgcc
     2161 cgcttcgtcg atcaggctca tggtctgcgc aaaatcatcc tcggtttcgc cggggaagcc
     2221 gacgatgaaa tcgctcgaca gcgaaatctc cggccgcacc gcgcgcaggc gcgcgatctt
     2281 gtccaggtac tgcgcgcgcg tgtactggcg cttcatcatg gacaggatgc ggtcgctgcc
     2341 gctttgtacc ggcagatgca gccagctcga cagcttgggc tcctcggcat aggcctcgat
     2401 caactcgtcg gtgaactcgg ccgggtgcga agtggtgtaa gcaatgcgct gcagcttcgc
     2461 atcgcgcgcc aggtagtgga tcagcagcgc gaggtcgcag gtctcggcgg tgtcgcccat
     2521 gcggccgcgg taggcgttga cgttctgccc cagcagcgtg atctcgcgca cgccctgcgc
     2581 ggtgagcgcg gcgcattcgg ccagcacgtc atccagtggg cggctcatct cggtgccgcg
     2641 cgtatacggc accacgcaga acgagcagta cttggagcag ccttccatga tcgagacgaa
     2701 ggcgcacgga ccttccgcgc gcggcgccgg caggcgatcg aacttctcga tctccgggaa
     2761 gctgatgtcc accgccggct tctgcgtggc gcgcgcgctg tcgagcagct gcggcaagcg
     2821 gtgcagcgtc tgcgggccga acaccaggtc aacctgcggc gcgcggcgcg tgatcagctc
     2881 accttcctgc gaggccacgc agccgcccac gccgatgacg cggccgggcg cggcctgctt
     2941 ccactccttc cagcggccga gctcggagaa caccttctcc tgcgccttct cgcgcaccga
     3001 gcaggtgttg agcagcagca ggtcggcctc gtcaggcgag tgggtgcgct cgtagccgtg
     3061 cgaggccttg agcacgtcgg ccatcttgga cgagtcgtac tcgttcatct ggcagccgta
     3121 ggtgatgagg tacagcttct tggacatcga agtcgctgga gaaacggccc cgaattatat
     3181 cggagcggca ccgccgcccg atgacagccg ctacaggcgc ttgagccagt ccgacacgcc
     3241 catctcgatc tcggcgtagg tctgctcgaa gtagctctgc ggcaggccgt aggggtcctc
     3301 gatgtcccga ttgccttccc atttcagcag cttatgcacg cggccgcgca gctgggggaa
     3361 acgcctggcg atccagcgct ggtgcgtctg gtcggccacc aggatgaggt cggccgcatg
     3421 cgcctgcccg tcggtgattt gcagggcgcc atggctggag atgtcgaaac catgcgccgc
     3481 catcaccgcc accgcctccg gtgccgccgg ctcgcccggc gacgcggcga tgccagccga
     3541 actcaccgac ttctccgcgg gcaggcggct gcgcatatag ccttcggcca tcgggctgcg
     3601 gcagatgttg ccggtgcaga cgatgaggat gcggttgaac atgattcact ccctctcccc
     3661 gcgagggcgg tgagggcatc agggaatgag caccactttg ccggtcacct tgcggtccat
     3721 catgtcgcgc agcgcctgcg cgccctgcgc cagcgggtac tggtgcgaca cgtgcggctt
     3781 gagcttgccc gccgcgtgcc acgcgaacag ctgctggaaa ttttccgcat tggccttggg
     3841 ttcgcgcgcc acgaacgcgc cccagaacac gccgaccgcg ctggcctgct tgagcagcaa
     3901 acgattgagc gcgatctgcg ggatggtgcc cgaggcgaaa ccaatcacca ggtagcggcc
     3961 gttccaggcg atggcggaaa agc
//
LOCUS       DASZZC010000230         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_919587,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000230 DASZZC010000000
VERSION     DASZZC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919587"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VHE37_05010"
     CDS             complement(<1..640)
                     /locus_tag="VHE37_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT34916.1"
                     /translation="MNIDLNDLRYFALIVEHGGFSAAERQTHITNSKLSRRMALLEDR
                     LGVRLLQRSTRRLALTEAGRAFHEHCRAMLVEAEAAQAAVDQLRAEPVGTVRITCPLA
                     MSQLYVARLLAEFMRKYPKVRIELDATDRVVNLIEERFDLALRARNLGLTDPSLTARR
                     IASGRLILVAGAAYAKKHRKLAHPEQAAQLDTIGPLSGGSEQHWELTDTRGDS"
     gene            747..1184
                     /locus_tag="VHE37_05015"
     CDS             747..1184
                     /locus_tag="VHE37_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVT34917.1"
                     /translation="MEQRLDFYKAGPAAIKAMLGLEQQIEKSGLEKPLIQLVRLRASQ
                     INGCAYCLDLHARDARKAGEDERRLATVSAWRETPFFSDRERAALEWTESLTEIARTQ
                     VPDEVWARVKPQFTPEEISDLTLLINAINGWNRYAIAFRKLPA"
     gene            1199..>1800
                     /locus_tag="VHE37_05020"
     CDS             1199..>1800
                     /locus_tag="VHE37_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT34918.1"
                     /translation="MNKVLLTSTFALTMLLDACVTAPADLPVAGAALEPAQLGLGTAP
                     AEIAASDWWAAYGDAQLDRLMRDALARNPTLAQALTRVREAQSLSDTAHAGLLPSLSF
                     DADETRAKLSGQDIIPPPYAGTTRWRGAEGLDLSWDLDFWGRQSSLLKQAHANADAAA
                     LDAAGARLAIAGAVLKTYIELDHQYALADVAQRSVQQREQT"
BASE COUNT          301 a          621 c          591 g          287 t
ORIGIN      
        1 ggctgtcgcc gcgcgtgtcg gtcagttccc agtgctgctc gctgccgccg gacagcggac
       61 cgatggtgtc gagctgcgcc gcctgctcgg ggtgcgcgag cttgcggtgc tttttggcgt
      121 aggccgcgcc ggccaccagg atcaggcggc cggaagcgat gcggcgcgcg gtgaggctcg
      181 gatcggtcag gcccaggttg cgcgcgcgca gcgcgagatc gaaacgctcc tcgatcaggt
      241 tgaccacgcg gtcggtggca tcgagctcga tgcgcacctt cggatacttg cgcatgaact
      301 ccgccagcag ccgcgccacg tacagctgcg acatggccag cggacaggtg atgcgcacgg
      361 tgcccaccgg ctccgcgcgc agctggtcga ccgccgcctg cgccgcctcg gcctccacca
      421 gcatggcgcg gcaatgctcg tggaacgcgc ggcccgcctc ggtcagcgcc agccggcgtg
      481 tgctgcgctg gagcaggcgc acgcccaggc gatcttccag cagcgccatg cggcggctga
      541 gcttggaatt ggtgatgtgc gtctgccgct cggccgcgga aaagccgccg tgctccacga
      601 tcagggcgaa gtaacgcagg tcgttcagat cgatgttcat gcaatcatcc tattttcagg
      661 acaatcattt gtaaaactgc cgtcttatcg ccgattgccc atatccatat cgtaagcgcc
      721 atcgaaaacg acgatggagt accgacatgg aacagcgtct cgacttctac aaggccggcc
      781 ccgccgccat caaggcgatg ctcgggctgg agcagcagat cgaaaaatcc gggctggaga
      841 aaccgctgat ccagctggtg cgcctgcgcg cctcgcagat caacggctgc gcctactgcc
      901 tggacctgca tgcgcgtgat gcgcgcaagg ccggcgagga cgagcgccgc ctggccacgg
      961 tttccgcctg gcgcgaaacg ccgttcttct ccgaccgcga acgcgccgcg ctggaatgga
     1021 ccgagtcgct caccgagatc gcgcgtaccc aggtgccgga cgaggtgtgg gcgcgggtca
     1081 agccgcagtt cacgccggaa gagatcagcg atctgacgct gctgatcaac gccatcaacg
     1141 gctggaaccg ctacgccatt gcgttccgca agctgcctgc gtgaaccgga gcatcgccat
     1201 gaacaaggtc ctgctgactt ccacgttcgc gctgaccatg ttgctggatg cctgcgtcac
     1261 cgcgccggcc gacctgcccg tcgccggcgc ggcgctcgaa cccgcgcaac tcggtctcgg
     1321 cactgcgccg gcggaaattg ccgcgagcga ctggtgggcg gcttacggtg acgcgcagct
     1381 cgaccgcctg atgcgcgacg cgctggcacg caaccccacg ctggcgcagg cgctgacacg
     1441 cgtgcgcgag gcgcagtcgc tttccgacac cgcgcatgcg ggcctgctgc cttcgctttc
     1501 cttcgacgcg gatgaaacac gcgccaagct gtccggccag gacatcatcc cgccgcccta
     1561 cgccggtacc acgcgctggc gcggcgcgga agggctcgat ctttcctggg acctggattt
     1621 ctggggccgg cagtcgtcgc tgctgaaaca ggcgcacgcg aatgccgacg ccgctgcgct
     1681 cgacgccgcc ggcgcacgcc tggccatcgc cggcgcagtg ctgaaaacct acatcgagct
     1741 cgatcatcaa tatgcgctcg ccgacgtggc ccagcgcagc gtgcagcagc gcgaacagac
//
LOCUS       DASZZC010000231         7441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_337497,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000231 DASZZC010000000
VERSION     DASZZC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337497"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VHE37_05025"
     CDS             <1..1543
                     /locus_tag="VHE37_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVT34919.1"
                     /translation="CLANPKPQQPWDRKDFKWPGGDSKHPANVQMWAIAPSMASAKSY
                     GNYPALTNIMSCVFEGGVVDFDTGCRIESRYFADLVVSQVAKNMIGTLFFQLNAINKG
                     KSRPEGFEKYTTKKLGVLGAGMMGAGIAYVSAKAGIDVVLLDTTQEAADRGKNYSADL
                     LDKAIKKGRETPMGKSAFLDKIKTTTNFDDLKDCDLIIEAVFEDRAIKADVTKKTEAV
                     IAKNAVFASNTSTLPITGLATASERPKNFIGLHFFSPVDKMPLVEIIKGKKTSPETLA
                     RGFDYVQQIRKVPIVVNDSRGFYTSRVFGTYVTEGAALLLDGQHPRSIEMAGMQAGMP
                     VGPLAVHDEVSLSLSLHILEQTRKDFAAEGKPFPEHPANAVTEKMVRELGRAGKKAGK
                     GFYDYPQGGQKHLWPELTKHFPLKDQLPQQELIDRLMFVQANETAKCYEEGVLESVAD
                     ANIGSIMGWGFAPFQGGALQFINAYGVDRFVKRARELEKKYGPRFKPARILVSMAKEG
                     QKFDA"
     gene            1536..2693
                     /locus_tag="VHE37_05030"
     CDS             1536..2693
                     /locus_tag="VHE37_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT34920.1"
                     /translation="MLNALLAQGSYVTDELRMFQETARKFMQAEVAPNVLKWDEQGCV
                     DRDLWNKAGELGLLCPSIPSEYGGGDGHFGFEKILIEEQGKAGCSAWGLSLHNGIVAH
                     YILHYGTEEQKMKWLPRLASGELVSAIAMTEPGTGSDLQSVRTTAIPEGDEYVVNGSK
                     VFITNGQQAGLIIVVAKTNPDEKAKGVSLLVVETDKVTGFRRGRNLEKVGMHGQDTSE
                     LFFENVRIPKSNLLGKAEGQGFVQLMQQLPQERLIVAIAAVAATELALDMTTQYVKDR
                     KAFGKRILDFQNTRFSLAEARTEAMLGRVLVEKCTELLIDRKLDAVTAAMAKYWTTDK
                     QFEIADLCLQLHGGYGYMDEYPISRLWRDSRVQRIYGGTNEIMKELIGRFL"
     gene            2823..5072
                     /locus_tag="VHE37_05035"
     CDS             2823..5072
                     /locus_tag="VHE37_05035"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVT34921.1"
                     /translation="MFAALMNWMKANKILPQISDTERQALEAGHVWIDGDFFRGAPDF
                     TRMMAQPYGKLSAEEQAFLDGPVEELLNMIDRYEIARTKRVPEHVIEFIKQQGFMGLI
                     IPRQYGGREFSTLAVSTIMHKVSPVCTAVSTFVVIPNSLGAAELLKHYGTEEQKNHYL
                     PKLASGEYVPCFGLTEPTAGSDAASIKAEGHVFKDHDGTVKIKLNFRKRYITLAPAAN
                     LISLACKLHDPANLLGKGADIGITVVMIHKGTPGFSNGDHHDPIGDSFENGPLLGKDV
                     IVPATQIIGGPAYAGLGWKMLMEQLAGGRAVSLPAGAVGGAKSVAAVTGPYSMVRQQF
                     GIPIGRMEGVEEKVGRIAALTYMVEGARVFGCSAVDAGIQPPVVSAILKAYTTQIGRE
                     LITDGMDVFSGAGVMQGPNNILGRGFAASPVGITVEGANIMTRTLMIFGQGATRCHPY
                     ALNVVKAVENNDAGAFRKNLLGWFGHFALGFLRTLLHGLTRGLFVGVPGVEPETRTYY
                     RRLGWAAARFGFLTDLAMFAIGGKLKARGKLTGRYADAIAWMVLGFSALRRYEAEGRR
                     GEDLPLVHYALQYALAETQKAFEGIYENFDGPLGAVLRTLGVLGLRLNPLATLPTDRL
                     SHQAALTIQHYNAQYKRLTDGVFFPRDESIATGRLVRAFRIVCEAHDAAERIIKAQHK
                     KKLPRGLPAEVAEQALAAGIIKAEEVALLKNALAARLEAIEVDVFTPEQYYIDGKQQA
                     PAMKLVANG"
     gene            complement(5248..6117)
                     /locus_tag="VHE37_05040"
     CDS             complement(5248..6117)
                     /locus_tag="VHE37_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34922.1"
                     /translation="MSTAKLLPLLLAGLLASPLVRADGASDLHARYGQSTQRLHDSPF
                     HRPLYLESQEESGKLQGEVYAEIEHPFAQVQQVLSDPQRWCEVLLLQPNVKQCRALEG
                     GSKLAVNLGRRFDQPIEKTYAAEFTFHPPQKQSDYFAVQLDAAKGPVGTHDYRIAVEA
                     MPVGSKTFLHLAYAYGYGMTARLATQAYFSTSGSGKVGFTVAGKSEDGTPAYVTGLRG
                     AVERNAMRYYLAIEAHLAVADTPQPQRFEQSLQRWFDSIEQYARQLKEDDRNAYVTVK
                     RGEYQRQQQAQQG"
     gene            complement(6186..6458)
                     /locus_tag="VHE37_05045"
     CDS             complement(6186..6458)
                     /locus_tag="VHE37_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34923.1"
                     /translation="MSEKLLELSSVVNVLSRTVGDLEKQRQSNETRSLVMSALMMALI
                     NTLPEERRAEVAKNFSSAADALMQASTSAGRAALQKEIASIHKMLA"
     gene            6651..>7441
                     /locus_tag="VHE37_05050"
     CDS             6651..>7441
                     /locus_tag="VHE37_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34924.1"
                     /translation="MTQDMQASRARKLPRAAALSVLLAAGAIACGPAYAVSDNDCKDE
                     QSAHITALLHDVHDPAEFASREDALRHSAEHFSDNIQTIIGEINAYSEAELPAATSKN
                     EADLAANQKTLDTFQSKPYSFPDNRFTPDYYERKVAVDQLKGCLMAKSQRERLAAATA
                     PVHAEPAAPAEPAAAVAAAQIAAMPLPPLPAPAVAAAPEAPPSTAAAEPAPAAAPAPA
                     APGCDAAAHEKLDQQLEQVRGRAEASMHTAECASPGGATPTLQVV"
BASE COUNT         1392 a         2471 c         2422 g         1156 t
ORIGIN      
        1 gtgcctggcc aaccccaagc cgcagcagcc gtgggaccgc aaggacttca agtggccggg
       61 cggcgattcc aagcacccgg ccaacgtgca gatgtgggcc atcgcgccgt ccatggcttc
      121 ggccaagagc tacggcaatt acccggcgct gaccaacatc atgtcctgcg tgttcgaggg
      181 cggtgtggtc gatttcgaca ccggctgccg tatcgagtcg cgctacttcg ccgacctggt
      241 ggtgtcgcag gtggcgaaga acatgatcgg cacgctgttc ttccagctga acgcgatcaa
      301 caagggcaag agccggccgg agggcttcga gaaatacacg acgaaaaagc tcggcgtgct
      361 cggcgccggc atgatgggcg cgggcatcgc gtacgtgtcg gccaaggccg gcatcgacgt
      421 ggtgctgctc gataccacgc aggaagccgc cgaccgcggc aagaactatt cggctgacct
      481 gctcgacaag gcgatcaaga agggccgaga gacgccgatg ggcaaatcgg cattcctgga
      541 caagatcaag accaccacga atttcgacga cctcaaggac tgcgacctga tcatcgaggc
      601 ggtgttcgag gaccgcgcga tcaaggcgga tgtaaccaag aagacggaag cggtcatcgc
      661 caagaacgcg gtgttcgcct cgaacacgtc caccctgccg atcaccggcc tggccaccgc
      721 cagcgagcgg ccgaagaatt tcatcggcct gcatttcttc tcgccggtgg acaagatgcc
      781 gctggtggaa atcatcaagg gcaagaagac ctcgcccgaa acgctggcgc gcggcttcga
      841 ctacgtgcag cagatcagga aggtaccgat cgtggtcaac gactcgcgcg gcttctacac
      901 ctcgcgcgtg ttcggcacct atgtcaccga gggcgccgcg ctgctgctcg acggccagca
      961 cccgcgctcg atcgagatgg ccggcatgca ggccggcatg cccgtcgggc cgctggcggt
     1021 gcacgacgaa gtctcgctgt cgctgtcgct gcacatcctc gaacagacgc gcaaggattt
     1081 cgccgcggag ggcaagccct tccccgagca cccggccaac gcggtcacgg aaaaaatggt
     1141 cagggaactc ggccgtgccg gcaagaaggc gggcaagggt ttctacgact acccgcaggg
     1201 cggacagaag cacctgtggc cggaattgac caagcatttc ccgctgaagg accagctgcc
     1261 gcaacaggaa ttgatcgatc gcctgatgtt cgtgcaggcc aatgagaccg ccaagtgcta
     1321 cgaggaaggc gtgctcgaat cggtcgcgga tgcgaacatc ggctcgatca tggggtgggg
     1381 cttcgcgccg ttccagggcg gcgcgctgca gttcatcaat gcctacggcg tggacaggtt
     1441 cgtcaagcgc gccagggaac tcgagaagaa atacgggccg cgcttcaagc cggcacgcat
     1501 cctcgtcagc atggccaaag aggggcaaaa gttcgatgct taacgcactg ctcgcacaag
     1561 gcagctacgt caccgacgag ctgcgcatgt tccaggaaac cgcccgcaag ttcatgcagg
     1621 cggaggtggc gcccaacgtg ctcaagtggg acgagcaggg ctgcgtggac cgcgacctgt
     1681 ggaacaaggc cggcgaactc ggcctgctct gccccagcat cccgtcggaa tacggcggcg
     1741 gcgacggcca cttcggcttc gagaagatcc tgatcgagga gcagggcaag gccggctgct
     1801 cggcgtgggg cctgtcgctg cacaacggca tcgtggcgca ctacatcctg cactacggca
     1861 ccgaggagca gaagatgaag tggctgccca ggctcgcatc cggcgagctg gtgtcggcca
     1921 tcgccatgac cgagccgggc accggctccg acctgcagag cgtgcgcacc acggcgatcc
     1981 ccgagggcga cgagtacgtg gtcaacggct ccaaggtgtt catcaccaac ggccagcagg
     2041 ccgggctgat catcgtggtg gccaagacca atcccgacga gaaggccaag ggcgtgtcgc
     2101 tgctggtggt ggagaccgac aaggtcaccg gcttccgccg cggacgcaat ctcgagaagg
     2161 tgggcatgca cggccaggac acctcggagc tgttcttcga gaacgtgcgc attcccaagt
     2221 ccaaccttct gggcaaggcc gaaggccagg gcttcgtgca gctgatgcag cagttgccgc
     2281 aggaacgcct gatcgtggcg attgccgcgg tcgccgccac ggaactggcg ctggacatga
     2341 ccactcaata cgtcaaggac cgcaaggctt tcggcaagcg catcctcgat ttccagaaca
     2401 cgcgcttctc gctggccgag gcgcgcaccg aggcgatgct cggacgcgtg ctggtggaga
     2461 aatgcaccga actgctgatc gaccgcaagc tcgatgccgt caccgccgcc atggccaagt
     2521 actggaccac cgacaagcag ttcgagatcg ccgacctgtg cctgcagctg cacggcggct
     2581 acggctacat ggatgaatac ccgatctcgc ggctgtggcg cgactcgcgc gtgcagcgca
     2641 tctacggcgg caccaacgag atcatgaagg aactgatcgg acggttcctc taagcattgc
     2701 tgtcattccc gcgaaggcgg gaatccagtt tggccgttga gcgtccaata aaactggata
     2761 cccgataccc gcctgcgcgg gcatgacgaa tcaaggattc ttttcagcct gtaggagttt
     2821 ttatgttcgc agccctgatg aactggatga aggccaacaa gatcctgccg cagatttcgg
     2881 ataccgaacg gcaggcgctc gaagcgggcc acgtgtggat cgacggcgat ttcttccgcg
     2941 gcgcgccgga cttcacgcgg atgatggccc agccctacgg caagctgtcg gccgaagagc
     3001 aggccttcct cgacgggccg gtggaagaac tgctcaacat gatcgaccgc tacgagatcg
     3061 cgcgcaccaa gcgcgtgccc gagcacgtga tcgagttcat caaacagcag ggcttcatgg
     3121 ggctgatcat ccccaggcag tacggcggac gggaattctc cacgctggcc gtctccacca
     3181 tcatgcacaa ggtcagcccg gtatgtaccg cggtcagcac cttcgtggtc attcccaact
     3241 cgctcggcgc ggcggaactg ctcaagcact acggcaccga ggagcagaag aaccactacc
     3301 tgcccaagct agccagcggc gaatacgtgc cctgcttcgg cctgaccgag ccgaccgccg
     3361 gctccgatgc cgccagcatc aaggccgagg gccacgtgtt caaggaccac gacggcacgg
     3421 tcaagatcaa gctgaatttc cgcaagcgct acatcacgct ggcgccggcg gccaatctca
     3481 tctcgctggc ctgcaagctg cacgacccgg ccaacctgct gggcaagggc gcggacatcg
     3541 gcatcaccgt ggtcatgatc cacaagggca cgccgggctt ttccaacggc gaccaccatg
     3601 acccgatcgg cgattcgttc gagaacggcc cgctgctggg caaggacgtt atcgtccccg
     3661 ctacgcagat catcggcggc ccggcctacg ccggcctggg ctggaagatg ctgatggagc
     3721 agctcgcggg cggccgcgcg gtgtcgctgc ccgcgggcgc ggtcggcggg gccaagagcg
     3781 tggccgcggt caccggcccg tactcgatgg tgcgccagca gttcggcatt cccattggcc
     3841 gcatggaagg cgtggaggag aaagtgggcc gcatcgccgc gctgacttat atggtggaag
     3901 gcgcgcgcgt gttcggctgc tcggcggtcg acgccggcat ccagccgccg gtggtctcgg
     3961 ccatcctcaa ggcctacacc acgcagatcg gccgcgagct gatcaccgac ggcatggacg
     4021 tgttctccgg cgccggcgtg atgcagggcc cgaacaacat cttgggccgc ggcttcgccg
     4081 cttcgccggt cggcattacg gtggaaggcg ccaacatcat gacgcgcacg ctgatgatct
     4141 tcggccaggg cgccacgcgc tgccatccgt acgcgctcaa cgtggtcaag gcagtggaga
     4201 acaacgatgc cggcgcgttc cgcaagaacc tgctgggctg gttcggccac tttgcgctgg
     4261 gcttcctgcg cacgctgctg cacgggctga cccgcggcct gttcgtcggc gtgcccggcg
     4321 tggagccgga aacgcgcacc tactaccggc gcctgggctg ggccgcggcg cgcttcggct
     4381 tcctcaccga cctcgccatg ttcgctatcg gcggcaagct gaaagcacgc ggcaagctca
     4441 ccggccgcta cgccgatgcc atcgcctgga tggtgctcgg cttctccgcg ctgcgccgct
     4501 acgaggcgga aggccgccgc ggcgaagatc tgccgctggt gcactacgcg ctgcagtacg
     4561 cgctggccga aacgcagaag gccttcgaag gcatctatga aaacttcgac ggcccgctcg
     4621 gcgccgtgct gcgcacgctg ggcgtgctcg gcctgcggct gaacccgctg gcgaccctgc
     4681 cgacggacag gctctcgcac caggccgcgc tcaccatcca gcactacaac gcgcagtaca
     4741 agcgcctcac cgacggcgtg ttcttcccca gggacgagag catcgccacc ggccgcctgg
     4801 tgcgcgcgtt ccgcatcgtg tgcgaggcgc acgatgccgc cgagcgcatc atcaaggcgc
     4861 agcacaagaa gaagctgccg cgcggactgc cggcggaagt ggccgagcag gcgctggccg
     4921 cgggcatcat caaggccgag gaagtggcgc tgctgaagaa cgcgctggcc gcgcgcctgg
     4981 aagcgatcga ggtcgacgtg ttcacgccgg agcagtacta catcgacggc aagcagcagg
     5041 cgccggcgat gaagctggtg gccaacggct gagactcacg ccgaaggaaa cgagcccgcc
     5101 gcgggaaacc cggcgggctt tttgctggcc gcgcaaaaaa aaacagatcc accgcaccgg
     5161 catttctcat gttataaaaa attatctatt tttatatata gacaataatt aataacatcg
     5221 gaaaccgggg tgcggtgcag ggttttttca gccctgctgc gcctgctgct gccgctggta
     5281 ctcgccgcgc ttcaccgtga cgtaggcgtt gcggtcgtct tccttcagct ggcgcgcgta
     5341 ctgctcgatg ctgtcgaacc agcgctgcag gctctgctcg aagcgctgcg gctgcggcgt
     5401 gtcggccacg gccaggtgcg cctcgatggc caggtagtag cgcatggcgt tgcgctccac
     5461 cgcgccgcgc agcccggtga cgtacgcagg cgtgccgtcc tcgctcttgc cggcgacggt
     5521 gaagcccacc ttgccgctgc ccgaggtgga gaaatacgcc tgcgtcgcca agcgcgcggt
     5581 catgccgtag ccgtaggcgt acgccaggtg caggaaggtc ttgctgccga ccggcatcgc
     5641 ttccaccgca atgcggtagt catgcgtgcc caccgggccc ttggccgcgt cgagctgcac
     5701 ggcgaaatag tccgactgtt tctgtggcgg gtgaaaggtg aattccgcgg cgtaggtttt
     5761 ctcgatcggc tggtcgaagc ggcggccgag attcaccgcg agtttgctgc cgccctccag
     5821 cgcgcggcat tgcttcacgt tgggctgcag cagcagcact tcgcaccagc gctgcggatc
     5881 ggacagcacc tgctgcacct gcgcaaacgg atgctcgatc tccgcgtaca cctcgccctg
     5941 cagcttgccg gattcttcct gcgattcgag atacagcggg cgatggaacg ggctgtcgtg
     6001 caggcgctgg gtcgactggc cgtagcgcgc gtgcaggtcg gaagcgccat ccgcgcgcac
     6061 cagcggcgac gccagcaggc cggccagcag caaaggcagt aattttgcgg tgctcatgcg
     6121 gatcagaccc gcgcgcggcg gatttgtccg cccaccgcgg cgtagatatt ccgcagcagc
     6181 gccgctcagg cgagcatctt gtggatgctg gcgatctcct tctgcagcgc cgcgcggccc
     6241 gcgctggtcg aagcctgcat cagcgcatcg gcggcggaag agaaattctt cgccacctcg
     6301 gcgcgccgct cctcgggcag cgtgttgatc agcgccatca tcagcgcgct catcaccagc
     6361 gagcgggttt cgttgctctg gcgctgcttc tccaggtcgc ccaccgtgcg cgacagcacg
     6421 ttgaccaccg atgacagctc cagcagcttt tcgctcatgt cccgtgcccg atcgagttcg
     6481 acaaccggcc ccactctagc atcgccctgc gcagcgattg gcagcgcgcg gccacggcct
     6541 tagactcgac gcacgcaatg cgccgccggc aggctggatc gcccgcggca tgggaagcat
     6601 tgcgcatttg ctcggggcgc agggacggcc cggccaaggg gagaagacag atgacgcaag
     6661 acatgcaggc gagccgcgct cgcaaattgc cgcgcgcggc agcgctttca gtgctgctgg
     6721 ccgcgggcgc catcgcttgc ggccccgcct acgcggtcag cgacaacgac tgcaaggacg
     6781 agcagagcgc gcacatcacc gcgctgctgc acgacgtgca cgacccggcg gagttcgcca
     6841 gccgcgagga cgcgctgcgg cacagcgccg agcatttcag cgacaacatc cagaccatca
     6901 tcggcgagat caacgcctac tcggaggcgg agctgcccgc cgccacgagc aagaacgagg
     6961 ccgacctggc cgccaaccag aagacgctcg acaccttcca gagcaagccc tactcgttcc
     7021 ccgacaaccg cttcacgccg gactactacg agcgcaaggt ggcagtcgac cagctcaagg
     7081 gctgcctgat ggccaagagc cagcgcgagc gcctcgcggc cgccacggcg ccggtgcatg
     7141 ccgagcccgc ggcgcccgcg gaaccggctg ccgcggtggc cgctgcccag atcgcggcga
     7201 tgccgcttcc cccgcttccg gcaccggcgg tcgcggccgc ccccgaggcg cccccgtcga
     7261 ccgcggctgc cgagcccgcg ccagccgccg caccggcacc cgccgcgccc ggctgcgatg
     7321 ccgccgcgca cgagaagctg gaccagcagc tggagcaggt ccgcggccgc gcggaggcgt
     7381 cgatgcacac agcggaatgc gcctcgcccg gcggtgccac gccgacgctg caggtggtga
     7441 t
//
LOCUS       DASZZC010000232         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_532752,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000232 DASZZC010000000
VERSION     DASZZC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532752"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1601)
                     /locus_tag="VHE37_05055"
     CDS             complement(<1..>1601)
                     /locus_tag="VHE37_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HVT34925.1"
                     /translation="GTLGGALALAAASGAWIWHNTHQLNRYTSAQDRQRMQADYEKRY
                     KALEYAPQPHVTEVDLNADLYPGELRARLRGTYTLVNRSEQPIADFYVLLPPQVQVEA
                     INADLPLTLADQAPELGWRHYRLAQPLAAGATTRFRFDIGYAVHGFTNDGASDVVLGN
                     GTFVNGAEGREVARLPAFGYDASYELSSDRERKKFGLAPKERMRDLDDARGRNIGVDW
                     IDHYQATLSTDAGQTAITSGVLTRQWNEGGRSYFSYRMDGRSLALLTVLSGRYAVKRD
                     RWQGAAQSADIEINYHAGHEFDLDRMIAGVKDSLDYYTQHFSPYQYPLVRIVEFPRYE
                     RFAESFPNTIPYSEAIGFIARVDDSDPKDIDYPYFVTAHEMAHQWWAHQEMPADVQGA
                     EFITESLAEYSALLVLKHKYGDARMHRFLHYELDRYLFGRAGESKKEQPLMRADGPSY
                     LHYAKGSLALYALQDAIGEDAVNRALSAFVQAWRFRGPPYPISRDLLAELRKVTPPEQ
                     QYLIEDLFETITLYELRATSAHARK"
BASE COUNT          235 a          510 c          568 g          288 t
ORIGIN      
        1 cttgcgcgcg tgggcggagg tggcgcgcag ttcgtacagc gtgatggtct cgaacaggtc
       61 ttcgatcagg tactgctgct cgggcggcgt caccttgcgc agttccgcca gcaggtcgcg
      121 cgagatcggg tagggcgggc cgcggaagcg ccaggcctgc acgaatgccg acagcgcgcg
      181 gttcaccgcg tcttcgccga tggcgtcctg cagcgcatac agcgccagcg agcccttggc
      241 gtaatgcagg taactggggc cgtcggcgcg catcagcggc tgctccttct tgctctcgcc
      301 ggcgcggccg aacaggtagc ggtcgagttc gtagtgcagg aagcggtgca tgcgcgcatc
      361 gccgtacttg tgcttgagca ccagcagcgc cgagtactcg gccaggctct cggtgatgaa
      421 ttccgcaccc tgcacgtcgg ccggcatctc ctggtgcgcc caccactggt gcgccatctc
      481 gtgtgcggtg acgaagtacg ggtagtcgat gtccttgggg tcgctgtcgt ccacgcgcgc
      541 aatgaagccg atcgcctcgg aatagggaat ggtgttgggg aaggattcgg cgaagcgctc
      601 gtagcgcgga aactcgacga tgcgcaccag cgggtactgg tacggcgaaa agtgctgcgt
      661 gtagtagtcg agcgaatcct tgaccccggc gatcatgcgg tcgaggtcga attcgtggcc
      721 cgcgtggtag ttgatctcga tgtcggcaga ttgcgctgcg ccctgccagc ggtcgcgctt
      781 caccgcgtaa cggccggaca gcaccgtcag cagcgccagg ctgcgcccgt ccatgcggta
      841 gctgaaatag ctgcgtccgc cttcgttcca ctgccgcgtc agcacgccgg aggtgatggc
      901 ggtctggccg gcgtcggtcg acagcgtggc ctggtaatgg tcgatccagt ccacgccgat
      961 gttgcgcccg cgcgcatcgt cgagatcgcg catgcgctcc ttgggcgcca gcccgaactt
     1021 cttgcgctcg cggtcggagg aaagctcgta cgaggcgtcg tagccgaacg ccggcagccg
     1081 cgccacttcg cggccttcgg cgccgttgac gaaggtgccg ttgcccagca ccacgtcgct
     1141 ggcgccgtcg ttggtgaaac cgtgcacggc gtagccgatg tcgaaacgga agcgggtggt
     1201 ggcgccggcg gccagcggct gcgccaggcg gtaatggcgc cagccgagtt ccggcgcctg
     1261 gtcggccagc gtcagcggca ggtcggcatt gatcgcttcc acctgcacct gcggcggcag
     1321 cagtacgtag aaatcggcga tgggctgctc gctgcggttg accagcgtgt aggtcccgcg
     1381 caggcgcgcg cgcagttcgc ccggatacag gtcggcgttc aaatccacct cggtcacgtg
     1441 cggctgcggc gcgtattcca gcgccttgta gcgcttctcg taatccgcct gcatgcgctg
     1501 gcggtcttgc gccgaggtgt agcgattcag ctggtgcgtg ttgtgccaga tccacgcgcc
     1561 gctggcggcg gccagcgcca gcgcgccgcc gagcgtgccg c
//
LOCUS       DASZZC010000233         5951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1175460,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000233 DASZZC010000000
VERSION     DASZZC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175460"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5359)
                     /locus_tag="VHE37_05060"
     CDS             complement(<1..5359)
                     /locus_tag="VHE37_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HVT34926.1"
                     /translation="MPSLRLALRAAVTAAVFALAACHPGSRVSAPPPPAPSIWSQFVA
                     SHTAGMVSKDSSISVRFARDLVPPEKNGKDASDALKVEPAIAGTLLYSGKREIVLTPA
                     APLPPGQQYRVTLNPQALAGLPAKLDPYQFDFRVLSPDFSIKAAGIEAAPSGDDMVLR
                     GTLETADAEDGAVVEKLLIASIDNQPVALQWAHFNDNRTHYFSATGIKRATAPQQLHL
                     AWSGKSIGLETAGTRDLEIPQSGVFKVLDASLASDGRQSVQVRFSAALDAKQNLGSLV
                     RLGKVKVSTRIDGSTLYIYPDKPVIGEVDLQIDDALRNAHGGRLAAAWQSSLKFENEK
                     PQVRFAGRGVILPDADQLTIPLEGMNVHAVQVTAFQVYESDMGQFLQDNNLAGGSELH
                     RVGRYLWRKTIQLPAPQANQWTRYALDAGELLKKNPGGLYRLSLSIDRSDSLYACAKD
                     DAAKPVAVEKPFRNMDDNYETSSWEGSDEYYEYEGDSGGSNDWSERDDPCKDSYYRWG
                     QGTHDARNFAASNIGLLAKRGSGGALRVVATRLRDAAVLSGAQIEVRNFQNQKIGGAV
                     TDADGFAAIELNSTPFYLVARKDSDVGYLKLNHNGALPVSHFDTGGETVKAGLKGFIY
                     GERGVWRPGDEIHLDFVLLDRTQRLPPDHPVTMQLYDPKGRLVQSITNTTPLDGFYAY
                     TLSTASDAPTGTWTAKALVGGAEFTRALKIETVMPNRLKMELKFAGASLSKQAMPLDG
                     TLWAQWLHGASASGLKADIAGKLDPVPTHFTSYTDYIFDDPARSFSGELSTLFEGRLD
                     ADGNAKVTLNLAPDSDPPGLMQASFTTRVFENGGAFSTAHSSFPYHAYPRYVGLKLPK
                     TDDWGYLAANTPFNIELAALDDDGKATDAQVQVSVYRIDWYWWWDRSEHSLAEYAQAG
                     THTPVLTQDFAVKDGHASGKLTVKSEDWGRYLVRACDTGGGHCTGKVVYVGYSGWAVK
                     SDDNGPGASALRFSSDKPKYTVGETATLQLPNATAGRALLSVENGSSILEQRWVDLSG
                     DKPSFRLPITAPMAPNVYVSLTLVQPHSGRDNDRPLRLYGIVPIEVTDPQTMLAPVIE
                     SDKLWRPSRTVSVQVSEHDGREMTYTLAVVDEGLLGLTSYKTPNPHDAFYQREALGVS
                     TWDLYDDVAGAYGGELERLLALGGDQGGPITERKEEKRFPPVVRVYGPFHLAAGEKQK
                     RDIDLPEYIGEVRVMVVAGHAGAYGSAEKSVTVREPLSLLATLPRVLGPNETVQVPVS
                     VFAYEPGIRDVKLSIETDALLENVGAPTQSLHFNAPGEQMAYFTLKVKPNSGAAHVTV
                     RASAGAYASKSSIAIGVRPGNPVVTRQLRQVLQPGKETSFDVKTFGLAGTNAAALEVS
                     RVPPLDLERRLNYLIQYPHGCIEQTTSSVFPQLYLDQLIELSDARKADIERNINAAIE
                     RLRSFQTASGAFSYWPGDSTISGWANDYAGHFLTEAKRLGYHVPDALYDGWLEFQRRT
                     AENWAPNTVDYIDDQSYRLLTLALADRPDVGAMNRLRETGPLDNVARVQLAQAYAQIG
                     LNDAASALMHADIRLHPYGTPGPSYGSETRDRGILLLGLLAIGDLNQAERMAEDLSAD
                     LSSDNWYSTQSTAYGLLAMARFAGGKHAAETFSYSWRAGDGKPVDVTARHALSAQPLP
                     KLGNGGALTVKNNSQQPLFVHVLVKGSPLPGEEDGAAAEGLSLSVNYTRADGSPLEIK
                     SLAQGTDFVAHVTVGNHSAVNLDDLALSEIVASGWEIHNPRFEGESHPEEKQAGDDYE
                     SA"
     gene            complement(5660..>5951)
                     /locus_tag="VHE37_05065"
     CDS             complement(5660..>5951)
                     /locus_tag="VHE37_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34927.1"
                     /translation="MNKNLTLRMGNCHHRRYIPMLIELVRSGQIDPLQILTQVEPITD
                     AIAAYQAFDQRKPGWIKVCLKPEEDAKAGTRSHKLDKALEDTFPASDPATHF"
BASE COUNT          833 a         1982 c         2024 g         1112 t
ORIGIN      
        1 aggcgctctc gtaatcgtca cccgcctgct tttcctccgg gtggctttcg ccctcgaagc
       61 gcgggttgtg gatttcccag ccgctggcga cgatctccga cagcgccagg tcgtcgagat
      121 tcaccgcgct gtgattgccc acggtgacgt gcgcaacgaa gtcggtgccc tgcgccagcg
      181 acttgatctc cagcggcgag ccgtcggcgc gggtgtagtt gaccgacagc gacagtcctt
      241 ccgccgccgc gccgtcctcc tcgcccggca gcgggctgcc cttgaccagc acgtgcacga
      301 acagcggctg ctgcgaattg ttcttcaccg tcagcgcgcc gccgttgccc agcttcggca
      361 gcggctgcgc cgacagcgcg tggcgcgcgg tgacgtccac cggcttgccg tcgcccgcgc
      421 gccagctgta gctgaaggtt tccgccgcgt gcttgccgcc ggcgaagcgc gccatcgcca
      481 gcaaaccgta ggcggtgctc tgcgtgctgt accagttgtc cgacgacagg tcggccgaca
      541 ggtcctcggc catgcgctcg gcctggttga ggtcgccgat ggccagcagg cccagcagca
      601 ggatgccgcg gtcgcgcgtc tcggagccgt agctcggccc cggcgtgccg tacggatgca
      661 gcctgatgtc ggcatgcatc aatgcgctgg ccgcgtcgtt gaggccgatc tgcgcgtagg
      721 cctgcgccag ctgcacgcgc gccacgttgt ccagcgggcc ggtttcgcgc aggcggttca
      781 tcgcgcccac atcgggccgg tcggccagcg ccagcgtgag caggcggtac gactggtcgt
      841 cgatgtagtc gaccgtgttc ggcgcccagt tctccgccgt gcgccgctgg aattcgagcc
      901 agccgtcgta cagcgcatcc ggcacgtggt agccgagccg cttcgcctcg gtgaggaaat
      961 gcccggcgta gtcgttggcc cagccgctga tggtgctgtc gcccggccag taggagaacg
     1021 cgccgctggc ggtctggaag ctgcgcaggc gctcgatcgc cgcgttgatg ttgcgctcga
     1081 tgtcggcctt gcgcgcgtcg gacagttcga tcagctggtc gagatacagc tgcgggaaca
     1141 ccgacgaggt ggtctgctcg atgcagccgt gcggatattg aatgaggtaa ttcaggcgcc
     1201 gctcgagatc cagcggcggc acgcggctca cttccagcgc ggctgcattg gtgccggcca
     1261 ggccgaaggt cttcacgtcg aaactggttt ccttgcccgg ctgcagcacc tggcgcagct
     1321 ggcgcgtgac caccgggttg cccggcctga cgccgatggc gatgctgctc ttgctcgcgt
     1381 acgcacccgc gctcgcacgc acggtcacgt gcgcggcgcc gctgttcggc ttcaccttga
     1441 gcgtgaagta cgccatctgc tcgcccggcg cgttgaagtg cagtgactgc gtgggcgcac
     1501 cgacgttctc cagcagcgca tcggtttcaa tcgacagctt cacgtcgcgg atgcccggtt
     1561 cataggcgaa caccgacacc ggcacctgca ccgtctcgtt cggcccgagc acgcgcggca
     1621 gcgtggccag cagcgacagc ggctcgcgca cggtgaccga tttttcggcc gatccatacg
     1681 cgccggcgtg gccggccacc accatcacgc gcacttcgcc gatgtattcc ggcaggtcga
     1741 tgtcgcgctt ctgcttctcg ccggcggcca gatggaacgg gccatacacg cgcaccactg
     1801 gcgggaagcg cttttcttcc ttgcgctcgg tgatcggccc gccctggtcg ccgcccagcg
     1861 cgagcaggcg ttcgagctcg ccgccgtagg cgccggcgac gtcgtcgtac aggtcccagg
     1921 tggacacgcc gagggcctcg cgctggtaga acgcgtcgtg tgggttgggc gtcttgtacg
     1981 aggtgaggcc gagcaggcct tcatcgacca ccgccagcgt gtaggtcatt tcgcggccat
     2041 cgtgctcgct cacctgcacg ctcaccgtgc ggctgggccg ccacagcttg tcgctctcga
     2101 tcaccggcgc cagcatggtc tgcggatcgg tcacttcgat cggcacgatg ccgtacaggc
     2161 gcagcgggcg gtcgttgtcg cgcccgctgt gaggctgcac cagcgtgagc gacacgtaga
     2221 cgttgggcgc catcggtgcc gtgatcggca gcctgaagct cggcttgtcg ccggacaggt
     2281 cgacccagcg ctgctccagg atgctgctgc cgttctcgac cgacagcagc gcgcggccgg
     2341 cggtggcatt gggcagttgc agcgtggcgg tttcgcccac cgtgtatttc ggcttgtcgg
     2401 aggagaagcg cagcgcgctg gcgccggggc cgttgtcatc gctcttcacc gcccagccgc
     2461 tgtagcccac gtacaccacc ttgccggtgc agtggccgcc gccggtgtcg caggcgcgca
     2521 ccaggtagcg gccccagtct tccgacttca ccgtgagctt gccgctggcg tggccgtcct
     2581 tcaccgcgaa gtcctgcgtc agcaccggcg tgtgcgtgcc ggcctgggcg tattcggcca
     2641 gcgagtgttc gctgcggtcc caccaccagt accagtcgat gcggtacacc gacacctgca
     2701 cctgcgcgtc ggtggccttg ccgtcatcgt ccagcgcggc cagctcgatg ttgaagggcg
     2761 tgttcgccgc caggtaaccc cagtcgtcgg tcttgggcag cttcagcccg acataacgcg
     2821 ggtaggcgtg atacgggaac gaggagtgcg cggtggaaaa cgcgccgccg ttctcgaaca
     2881 cgcgcgtggt gaagctggcc tgcatcagcc cgggcggatc gctgtccggc gccaggttga
     2941 gcgtgacctt ggcgttgccg tcggcatcaa gccgcccttc aaacagcgtg ctgagctctc
     3001 cggaaaaact gcgtgcggga tcgtcgaaga tgtagtcggt gtacgaggtg aaatgcgtcg
     3061 gcaccgggtc gagcttgccg gcgatgtcgg ccttgagccc gctggcgctg gcgccgtgca
     3121 gccactgcgc ccacagcgtg ccgtcgagcg gcatggcctg cttcgacagc gatgcaccgg
     3181 cgaacttcag ttccatcttg agccggttgg gcatcaccgt ctcgatcttc agcgcgcgcg
     3241 tgaattccgc gccgccgacc agcgccttgg ccgtccaggt gccggtgggc gcgtcgctcg
     3301 cggtgctcag cgtgtaggcg tagaagccat cgagcggcgt ggtgttggtg atgctctgca
     3361 ccagccggcc cttggggtca tacagctgca tggtcaccgg gtggtccggc ggcagccgct
     3421 gcgtgcggtc gagcagcacg aagtcgagat ggatctcgtc gccgggccgc cacacgccgc
     3481 gctcgccgta gatgaagccc ttgagcccgg ccttgaccgt ttcgccgccg gtgtcgaaat
     3541 gcgacaccgg cagggcgccg ttgtggttga gcttcaggta gccgacgtcg ctgtccttgc
     3601 gcgccaccag gtagaacggc gtgctgttca gttcgatggc ggcgaagccg tcggcgtcgg
     3661 tgaccgcgcc gccgatcttc tggttctgga aattgcgcac ctcgatctgc gcgccggaca
     3721 gcaccgccgc atcgcgcagg cgcgtggcca ccacgcgcag cgcgccgccg ctgccgcgct
     3781 tggccagcag gccgatgttg gaggcggcaa agttgcgcgc gtcgtgcgtg ccctggcccc
     3841 agcggtagta gctgtccttg cacgggtcgt cgcgctccga ccagtcgttg ctgccgccgc
     3901 tgtcgccctc gtactcgtag tactcgtcgc tgccttccca gctgctggtc tcgtagttgt
     3961 cgtccatgtt gcggaacggc ttttccaccg caacgggttt cgcggcgtcg tccttggcgc
     4021 aggcgtagag cgaatcgctg cggtcgatcg acagcgacag ccggtacagc ccgcccgggt
     4081 ttttcttcag cagttcgccc gcgtccagcg cgtagcgcgt ccactggttg gcctgcggcg
     4141 ccggcagctg gatggtcttg cgccacaggt agcggcccac gcggtgcaac tcgctgccgc
     4201 cggccaggtt gttgtcctgc aggaactggc ccatgtcgct ctcgtacacc tggaacgccg
     4261 tcacctgcac cgcgtgcacg ttcatgcctt ccagcgggat ggtcagctgg tccgcatccg
     4321 gcaggatcac gccgcggccg gcgaagcgca cctgcggttt ctcgttctcg aacttcaggc
     4381 tgctctgcca ggccgcggcc agccggccgc cgtgcgcgtt gcgcagcgcg tcgtcgatct
     4441 gcaggtccac ctcgccaatc acgggcttgt ccgggtagat gtacagcgtg ctgccgtcga
     4501 tgcgcgtgct caccttgact ttgcccaggc gcaccagcga gccgaggttc tgcttggcat
     4561 cgagcgcggc ggagaagcgc acctgcaccg actggcggcc gtcgctggcc aggctggcgt
     4621 ccagcacctt gaacacgccg ctctgcggga tctccaggtc gcgcgtgccg gcagtttcca
     4681 gtccgatgct cttgccgctc cacgccaggt gcagctgctg cggcgcagtc gcacgcttga
     4741 tgccggtggc ggaaaaataa tgcgtgcggt tgtcgttgaa atgcgcccac tgcagcgcca
     4801 ccggctggtt gtcgatgctg gcgatgagca gtttctccac caccgcgccg tcttcagcgt
     4861 cggccgtctc cagcgtgcca cgcagcacca tgtcatcgcc gctgggtgcg gcctcgatgc
     4921 ctgccgcctt gatggagaag tcgggcgaca gcacgcggaa atcgaactgg tacggatcga
     4981 gcttcgccgg cagtccagcc agcgcctgcg ggttgagtgt gacgcggtac tgctggcccg
     5041 gcggcagcgg cgcggcgggc gtgagcacga tctcgcgctt gccgctgtac agcagcgtgc
     5101 cggcgatggc cggctccacc ttcagcgcgt ccgacgcgtc cttgccgttt ttctccggcg
     5161 gcaccaggtc gcgcgcgaag cgcacgctga tgctgctgtc cttgctcacc atgcctgcgg
     5221 tgtggctggc cacgaactgg ctccagatgc tgggcgccgg cggcggtggt gcgctgacgc
     5281 gcgatcccgg gtgacaggca gccagcgcga acacggcagc agtgacggcg gcgcgcagcg
     5341 caagacgtag agacggcatg gtgcaactcc ctggagagca cggttgggtt tgccgcggcg
     5401 tccgcccttt gcacgatctt gtcccgggac gccgcggccg gtgtggcgac aagtattgca
     5461 ccgggcaggc gtcatgctgc gtgcacgcgg cgcagggcgc gtggcgcagt cgtggaaaat
     5521 tggtgcaagc gcgtcagcgg tgcggcgtgc gtgccagaaa tggggtcacc gcaccgctat
     5581 ttttgatgtt attaaatatt gcctaattaa taattaatat aatatttaat aactcgtccg
     5641 aagcgggtgc ggtgaggctt cagaaatgcg tcgcagggtc gctcgccggg aaagtgtctt
     5701 ccagcgcctt gtctagtttg tgcgagcgcg tgcccgcctt ggcatcttcc tcgggcttca
     5761 gacacacctt gatccagccg ggcttgcgct ggtcgaacgc ctggtaggcg gcgatggcgt
     5821 cggtgatcgg ctccacctgc gtcaggatct gcagcgggtc gatctgcccc gagcgcacca
     5881 gctcgatcag catggggatg tagcggcggt gatggcagtt gcccatgcgc agggtcaggt
     5941 tcttgttcat t
//
LOCUS       DASZZC010000234         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_211508,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000234 DASZZC010000000
VERSION     DASZZC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211508"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHE37_05070"
     CDS             complement(<1..651)
                     /locus_tag="VHE37_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVT34928.1"
                     /translation="MALKLAVIGDVHGCWNDADTAYFNASDYSALLFVGDLAPLIGAL
                     PTARRLAALRKPAYLIPGNHDATGAAQFLAELRHRERLAAVLSFGQRSRVRALAAALG
                     PVKCVGYTLDLLEIDGRLLGLIAARPHSMGGDHFYFRSYLKRAYGVGSFEESAEKLRA
                     LVDAAPRDLLFLAHNGPAGFGAARDDLWGCDFRLQGGDFGDPDLQDAVRHARSQGKR"
     gene            complement(658..1539)
                     /locus_tag="VHE37_05075"
     CDS             complement(658..1539)
                     /locus_tag="VHE37_05075"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVT34929.1"
                     /translation="MTYKTLDWAVADGILTLTLNRPEQLNSFTVEMANELVAAFERAS
                     ADASVRAVVVTGAGRAFCAGMDLTRPGNVFGLDESQRPTLDDMHLRLREPDIEAGVRD
                     TGGRVTLAIFNCTKPVIAAINGAAVGIGATMTLAMDFRLASDKARIGFVFGKIGIVPE
                     ACSTWFLPRIVGISQALEWCYSGDILDAAEAQRGGLVKAVLPPDQLLPEAYKLARRLV
                     EHKSPVAVALTRQMMYRNSAKEHPLEAHRVDSLAVFYTSLHDGKEGVQSFLEKRAPQF
                     SARVPDDLPPFYADWAK"
BASE COUNT          296 a          612 c          652 g          262 t
ORIGIN      
        1 gcgcttgccc tgagagcgcg cgtggcgcac ggcgtcctgc aggtcgggat cgccgaagtc
       61 accgccctgc aggcggaagt cgcagcccca gaggtcgtcg cgcgcagcgc cgaatccggc
      121 cgggccgtta tgcgcgagga acagcaggtc gcgcggcgcg gcgtcgacca gcgcgcgcag
      181 tttctccgcg gattcctcga agctgcccac gccgtaggcg cgcttgaggt aggagcggaa
      241 atagaaatgg tcgccgccca tgctgtgcgg gcgcgcggcg atcaggccga gcaggcggcc
      301 gtcgatctcc agcaaatcga gcgtgtagcc cacgcacttc accgggccca gcgccgcggc
      361 cagcgcgcgc acacgcgagc gctggccgaa actcaacacg gccgccagac gctcgcgatg
      421 acgcagctct gccaagaact gcgcggcccc ggtggcgtcg tgattgccgg ggatcaggta
      481 cgccggcttg cgcagcgcgg ccagccggcg cgcggtgggc agcgcgccga tcagcggcgc
      541 aaggtcgccc acgaacagca gtgccgaata atcgctggca ttgaagtacg cggtatcggc
      601 gtcgttccag cagccgtgca cgtcaccgat gacggccagc ttcagcgcca tgcgcggcta
      661 cttcgcccag tccgcgtaga acggcggcag gtcgtcgggc acgcgcgcgg aaaactgcgg
      721 cgcgcgcttt tccaggaacg actgcacgcc ttccttgccg tcgtgcagcg aggtgtagaa
      781 caccgcgagc gagtcgaccc ggtgcgcttc cagcggatgc tccttggcgc tgttgcggta
      841 catcatctgc cgcgtcagcg cgacggccac cggcgacttg tgctccacca accggcgcgc
      901 cagcttgtac gcctcgggga gcaactggtc gggcggcagc acggccttga ccagtccgcc
      961 acgctgcgcc tcggcggcat cgaggatgtc gccggaatag caccactcca gcgcctgcga
     1021 aatgccgacg atgcgcggca ggaaccaggt ggagcacgct tccggcacga tgccgatctt
     1081 gccgaagaca aaaccgatgc gcgccttgtc gctggccagg cggaaatcca tggccagcgt
     1141 catggtggcg ccgatgccca ccgccgcgcc gttgatcgcg gcgatcaccg gcttggtgca
     1201 gttgaagatg gccagcgtga cgcggccgcc ggtgtcgcgc acgccggcct cgatgtcggg
     1261 ctcgcgcagg cgcaggtgca tgtcgtccag cgtcggccgc tgcgactcgt cgaggccgaa
     1321 cacgttgccc ggacgcgtga gatccatgcc ggcgcagaag gcgcggcccg cgccggtgac
     1381 caccaccgcg cgcacgcttg catcggcgct ggcgcgctcg aaagcggcaa ccagttcatt
     1441 ggccatctcc acggtgaagg aattgagctg ctcgggccgg ttgagtgtga gcgtcaggat
     1501 gccgtcggcg acggcccagt ccagggtctt gtaggtcatg tgcgcgggct tgaaagaggt
     1561 gcggcagtgt agcgcgcgta tatataagta aagtccctgg ggcggcggcg cgcggcagtg
     1621 ctagcgtggc cgcgttgcag ctggagcgtg acatgtccct ggaacgggca caggccgcgg
     1681 ccattgcgct gaaccgttac gggctcggcg cgcggccggg cgaggcggat cgcatcggcg
     1741 ccgatgcgcg cggctggctg cgcgcgcagc tcgacgcacg aaacgcgcag acgccgccat
     1801 cgatgcgcga gctgccggat tc
//
LOCUS       DASZZC010000235         8782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1044022,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000235 DASZZC010000000
VERSION     DASZZC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044022"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VHE37_05080"
     CDS             <1..420
                     /locus_tag="VHE37_05080"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HVT34930.1"
                     /translation="DSKHAVIDLAFADNLAFNHDRVANRGGGAVNNFGEGSGAFDGGN
                     RAYFAQVKVGDPVISYRWQWNVFFNYRRIETDAVVDAFNDSDFHLGGTNAKGYTVGGN
                     VALGRNTSLGLRWMNSTEVTAFPYAIDTVQIDLSSRF"
     gene            426..1028
                     /locus_tag="VHE37_05085"
     CDS             426..1028
                     /locus_tag="VHE37_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34931.1"
                     /translation="MKHRAALLGLLLPLMQVSLAVAADNNSAEARLREQLRQTAVALR
                     QLQDENGDLKTKLADAEAHAKSAGDDAANAQHALAQEKIRSTDLDRKLGDANSQLAQL
                     QVSYQAATKTISSATAQNDKLTADLGELQGRYGTCVSRNAELVNIGNQLVESYRNKGV
                     WAALLDAEPVTGLHRVELERAAQEFHARIIDSTLPAGSSP"
     gene            1028..>8782
                     /locus_tag="VHE37_05090"
     CDS             1028..>8782
                     /locus_tag="VHE37_05090"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HVT34932.1"
                     /translation="MPPRKTPRRVDGPFRLSAVGVLRRYVLASSLGLSAVTGTATAGG
                     PAFGSAAWLAIKNAQTAAVQTPGGAPSRGVAGAVTTAGAAQQQSTRTVQDLGRAADAI
                     VAAQNAQAAARDQLQQQPTSVPDGLGAGGLQAAAGAAQNVAAPASCAATNTCVWQNAA
                     LPTQSVANGQTTVTVTQNAQKAILTWDSFNVGAHTTLNFDQSAGRQADGSNNWVALNR
                     IGGDIAPSQILGQIKADGSVYLINPNGFIFGGTSTVNVHTLVATSLPMFSSNTTTSNG
                     VFLNQGIQGSGVTTLLGLAGDQQIASASQLPAASAITIDEGAQISTGELGFSLIAAPQ
                     VINHGTIQADQGQVILAAALGVSQAAVSVGASTGGANNLNPVLTGRYIVQGPGGPTDV
                     TPVTSLVNDGLIEASRGNVTLLGHDVTQSGVVVATTSITNAGSIALAAQDELSITDKA
                     VARTGSLVLAADSVTALLPEHDGETTTTNAAADAAFQRGSISAKGSAITVNAGALIEA
                     PGENVSLAAIAQDAGQVHPLIGDGSIAGRLYVDSGATIDVAGLADIELPASSLLVTIP
                     RLGQNELADSPLQRNGFLFGQSVVIDSRDTGTRSDGTTWNGTPLANLSGYVEQRARNI
                     DQMLQNAGNIGLAGNEVIVRSGSLLNLNGGYLHYLGGYLQTTQLIAADGSLVPVAGAD
                     PYRTYLGIAGVYTQSHPRWNVTSTFVDPLASGAGTYQSDYVQGGSAGTLSIFGQTTAL
                     LNGDINAHAFAGQDQVGRGQMPSGGKFFAGAGSALSAIFPDPTLLQHGPSYVLESGVP
                     DVGQLVAGFDAKTALPDPGSVITDSSNYLHWTPIASQQLTGAGFSSVTVAADDAGASN
                     LGGEIRVEQGGALTVQPGGSISLTGSRVVVLDDLVARAGSIKVVATGQTGLVGTTVQA
                     GSSASAPTSGDVVVGDGVALNASGLWVNDTGLDADQITGGAYINGGSISLQTLQNAVV
                     TTAKVAGCAAPCVLDTTGSILLSSGSLLDVSSGGRVQRNGKFVESDGVLQARGGNISL
                     QIYGLRNGKQFGSDVLPLPSAKPPQHGTLQLDGSLRGYGFSGGGTLAIQALGLQIGGT
                     SAASWNLNLAPEFFAGQGFGAYTLSAEYDATIGAGTVLNPVQSNLIPDRELLSLAATG
                     TDLYGHAQADPGRGYTTIGTLDAYHRQATSQSISAGDYLNWRLPGQDSTNPPDYRAAG
                     ITGTLLLDHGAVIQTDAGASVTLGSMNQLTIDGSIIDHGGNVTLTADTAGGGYAQLAN
                     ALAAYSSNSKSVWLGADSLIDVSGISILNPYASVIAQGANLVQSRTGRLYGGGNVTIS
                     NDTGYVVAVGCKEIACDVLQHGAIIDVRGSNDAYDLRTDSGGYARTPVWSDGGGITIG
                     AAAGLYFDAALHGDGGAASANGGSLTVTAENALPVTPASGFGGATQMVLSQQSPVFTA
                     GALTPGQNVEVTKGVPAGIVRFSADVLDGSGITSLQLGSDPVFDRTSAPAPITVSGNV
                     DLALGRSIVINTTSLIAAPLDASGSHLDLSAPYVALHGYVPGGDYPKLPVLTAPAAGT
                     QLAVQAQDIDLGGQLSLRNFADASFVSSDDLRFVTPSRDANYFSSNVLDPSQTVPGIL
                     ATAGNLEFQAARIYPASGNAYIIDAVGALTAGSGARAETTVSFLGNGQSATAATPLSA
                     GGSLLVDATHIIQSGNLRAPSGQIVLGVTDANDAVTQGLFGYTDSDPSGSARFVPLPL
                     VSTASVDLAAGSITSVSLDGLTVPYGQTVDGQNWQYNPAAAGVPGATSSLPDLTAPPG
                     KQITLGGAAINMASGSIVDLSGGGDLQGQEFVPGSGGTRDVLSQFQNSFGTSAPVPLY
                     PDSRMVYAVIPGYRGLAAYDPSIVAGSPLVGRSVYLSGGHGLPAGIYVLLPGKYATLP
                     GAFRVVQDTRVADAAAAQNAVLADGTQVIAGRFVDAFSGSSSARSTAFLVQAAPAWQL
                     YSQYNLTSANQYFSDFAQSSGTAIPLLATDAGHLVLAASSTLSLQGTVDTVPATGGIG
                     AEVDIAATRLAITAGGTAAAPGFVALDVNQLDQLQASSLLLGGTRTRTASGDLITALA
                     TDVVASNGDTALSAPEIMLVSKAGGGGIRVDAGSVIAAQGSIAANADVPIQIGSLPSS
                     GSGVSGDGSLLRVSNGADVIVNRVNVPGIDAAGTATGSLTLAAGAVVRADQSVTLDST
                     GSTAVDRTATFSARSISADAASVSFVSGTDPAATGLVVGADLLAQFASAEQITLSSRG
                     AMDFIGGVDLQVAQNLALNAAAFRTSGGDVSLDAATLTLGNALHGAGSGSNAGTHQLD
                     LHATQLVFGDGSASLDGFGQVNAVATRSTELRGQGRFDFGSAAVDLTTSLLSAAAGAD
                     HTLATTAAFSVSGTPAAGTAAAGGALRLQGGSLSVDTRLQSLGGVIDLHASTGDVALG
                     SSAQLSVAGTAQPFYDVTSYGSAGTIDLTSDQGSVTASAGSLLDFGSASGGGNAGRLN
                     VRARQGAAMLNGTLRGMGADAGGSFGLDTGGAIDLNALASQLAADGINQQIDLHSRSG
                     NLQLSTGHVLAAASVSLTADGSDSSSGMVQVDGTIDASGSSGGQIAL"
BASE COUNT         1493 a         2908 c         2917 g         1464 t
ORIGIN      
        1 gattccaagc acgcggtcat cgacctggca tttgccgaca acctggcttt caaccacgac
       61 cgcgtggcca accgtggcgg cggtgcggtc aacaatttcg gcgagggcag cggtgctttc
      121 gatggcggca accgcgccta cttcgcgcag gtgaaggtcg gcgacccggt gatctcctac
      181 cgatggcagt ggaacgtgtt cttcaactac cggcgcattg aaaccgacgc ggtggtcgac
      241 gcgttcaacg attcggactt ccacctgggc ggtaccaacg ccaagggcta caccgtgggc
      301 ggcaatgtcg cgctcggccg caacacctcg ctgggcctgc gctggatgaa ttcgaccgag
      361 gtgacggcat tcccctatgc catcgacacc gtgcagatcg acctgagctc gcgcttctga
      421 caaccatgaa acatcgagct gcattgctgg ggttgctgct gccgctgatg caggtttcgc
      481 tggcagtggc ggcggataac aactccgccg aagcccggct gcgcgagcag ttgcgccaaa
      541 ccgctgtggc gctgcgccag ttgcaggatg agaacggcga cctcaagacc aagctggccg
      601 atgccgaggc ccatgccaaa tcggcgggcg acgatgccgc gaatgcgcag catgcgctgg
      661 cgcaggagaa gattcgcagc accgatctcg accgcaagct gggcgatgca aattcccagc
      721 tggcacagtt gcaggtttct taccaggcgg cgaccaagac catcagcagc gccacggcgc
      781 agaacgacaa gctgacggcc gaccttggcg agctgcaggg ccgctacgga acctgcgtgt
      841 cgcgcaacgc agaactggtg aacatcggca accagctggt ggaaagctac cggaacaagg
      901 gcgtgtgggc agccctgctg gatgcggagc cggtgaccgg cctgcatcgc gtagaactgg
      961 aacgtgcagc gcaggaattc cacgcgcgca tcatcgattc caccttgccg gcgggcagca
     1021 gcccatgatg ccgccgcgca aaacgccgcg ccgggtggat ggacccttcc gcctttctgc
     1081 cgtgggtgtg ctgcggcgct acgtgctcgc cagcagcctc ggcttgtcgg cggtgaccgg
     1141 cacggcgacg gcgggcggcc cggcgttcgg cagcgcggcc tggctggcga tcaagaacgc
     1201 gcagacggct gccgtgcaga cgccgggcgg cgctcccagc agaggagtcg ccggtgcagt
     1261 gacgacggcc ggcgcggcgc agcagcagtc gaccaggacg gtgcaggatc tcggccgtgc
     1321 ggccgatgcc atcgtcgccg cgcagaatgc gcaagccgct gcgcgtgatc agctgcagca
     1381 gcaaccaaca tcggtgcccg atggactggg cgcgggcggt ttgcaggcgg cggcgggcgc
     1441 cgcgcagaac gtggcagcgc ccgcatcgtg cgccgcgacc aatacctgcg tctggcagaa
     1501 cgcggcgctg cccacgcaga gcgtcgccaa cggccagacc acggtcaccg tcacgcagaa
     1561 tgcgcagaag gcgatcctca cctgggattc gttcaacgtc ggtgcgcata ccacgctcaa
     1621 cttcgaccag agcgcgggcc ggcaggcgga cggcagcaac aactgggttg cgctgaaccg
     1681 catcggtggc gacatcgccc ccagccagat cctgggccag atcaaggccg atggctcggt
     1741 ctacctgatc aatccgaacg gattcatctt cggtggaacc agcacggtca acgtgcatac
     1801 gctggtggcc acatcgctgc cgatgttctc gtcgaacacg acgaccagca acggcgtgtt
     1861 cctcaatcag ggcatccagg gttcgggagt gaccacgctg ctgggcctgg ccggtgatca
     1921 acagatcgct tccgccagcc agctgcccgc cgcgagcgcc atcaccatcg acgaaggggc
     1981 gcagatcagc acgggcgagt tggggttttc gctgatcgcc gcgccgcagg tgatcaacca
     2041 cggcaccatc caggccgacc aggggcaggt gatcctggcc gcggcgctgg gtgtgtcgca
     2101 ggcggcggtc agtgttggtg cttcgaccgg cggcgccaac aatctcaacc cggtgctcac
     2161 cggccgctac atcgtgcagg gtccgggtgg tcccaccgat gtgacgccgg tgacttcact
     2221 ggtcaacgac ggcttgatcg aagcgtcgcg cggcaacgtc accttgctgg gccacgacgt
     2281 cacgcaatcg ggtgtggtgg ttgcgaccac cagcatcacc aacgccggca gcattgccct
     2341 ggcggcccag gacgaactgt cgatcacaga caaggcggtg gctcgtacgg gttcgctggt
     2401 gttggccgca gattcagtga cggcgctcct gcccgagcat gacggcgaga ccacgacgac
     2461 caatgccgcg gccgacgctg ctttccagcg cggcagcatt tccgcgaagg gcagcgcgat
     2521 caccgtcaac gccggtgcgc tgatcgaagc gccgggtgaa aacgtgtccc tcgcagccat
     2581 tgcgcaggac gcgggacagg ttcacccgct gatcggcgac ggctccattg ccggccggct
     2641 gtatgtcgac agcggcgcga ccatcgatgt ggctggcctg gcggacatcg agttgcctgc
     2701 gtcgtcgctg ctggtgacca tcccgcgcct ggggcagaac gagctggctg attcgccgct
     2761 gcagcgcaat ggcttcctgt tcggccagtc ggtcgtcatc gacagccgtg acaccggcac
     2821 gcgcagcgac ggcacgacct ggaatggcac gccgctggcc aacctgtcgg gttacgtcga
     2881 gcaacgcgca cgcaatatcg accagatgct gcagaacgcc ggcaacatcg ggctggccgg
     2941 caacgaagtg atcgtgcgca gcggctcgct gctcaatctg aacggcggct acctgcatta
     3001 tctgggcggc tacctgcaga ccacgcagct gattgcagcg gatggttcgc tggttccggt
     3061 tgccggcgcc gatccctatc gcacctacct gggcatcgcg ggcgtataca cgcagtcgca
     3121 tccgcgctgg aacgtgacct cgactttcgt cgatccgctg gcaagcgggg caggcaccta
     3181 ccagtcggac tacgtgcagg gcgggagcgc ggggacgctg agcatctttg gacagacgac
     3241 ggcgctgctc aatggcgaca tcaatgccca cgcgttcgcc ggacaggacc aggttggccg
     3301 cgggcagatg cccagtggcg gaaaattctt cgccggtgcg ggttctgcac tgtctgcgat
     3361 ctttcccgat cccacactgc tgcaacatgg gccgagctac gtgctcgagt ccggcgtgcc
     3421 ggatgtcgga cagctggtcg ccggcttcga tgccaagacc gcgttgccgg atcccggcag
     3481 cgtcatcacc gacagcagca actatctgca ttggacaccg attgcctcgc agcagttgac
     3541 cggcgccggc ttctccagcg tgaccgtcgc cgccgatgat gccggtgcaa gcaacctggg
     3601 cggcgagatc cgcgtggagc agggcggtgc attgaccgtg cagcccggcg gcagcatctc
     3661 tctcaccggt tcgcgcgtcg tcgtgctgga tgacctggtg gcgcgtgccg gatcaatcaa
     3721 agtggtggcg accggccaga ccggccttgt cggtaccacc gtgcaggcgg gatcgagcgc
     3781 atcagcgccc accagcggtg acgtcgtcgt gggagacggc gtcgcgctca atgcttcggg
     3841 cttgtgggtc aacgacaccg ggctcgatgc cgaccagatc accggcggtg cctacatcaa
     3901 tggcggcagc atttcgctgc agacgctgca gaacgcggtc gtcaccacgg caaaggttgc
     3961 gggctgcgct gcaccgtgcg tgctcgacac tacgggttcc attctgcttt ccagcggcag
     4021 cctgcttgat gtgtccagcg gtggccgcgt gcagcgcaac ggcaagtttg ttgagagcga
     4081 tggtgtgctg caggcccgcg gcgggaacat ctcgctgcag atctacggat tgcgcaatgg
     4141 caagcagttc ggttccgacg tgctgccttt gcccagcgct aagccaccgc agcatggcac
     4201 cttgcagctc gacggaagct tgcgcggcta cgggttctcc ggcggcggca cgctggccat
     4261 ccaggccctc ggactgcaga ttggcgggac gtcggcggcc agctggaatc tgaatcttgc
     4321 tcctgaattt ttcgcgggcc agggtttcgg tgcatatacc ctcagcgccg agtacgacgc
     4381 gacgatcggc gcgggcaccg tgcttaatcc ggtccagtcc aacctgattc cggatcgtga
     4441 actgctgtcg ctcgcggcca ccggcaccga tctctacggt catgcgcagg cagatccagg
     4501 ccgcggctac accaccatcg gcactctgga cgcttatcac cgccaggcga ccagccagtc
     4561 gatctctgcc ggcgactatc tcaactggcg cctgccgggc caggattcga ccaatccgcc
     4621 ggactatcgt gccgcgggca tcaccggcac cttgctgctc gaccatggtg ccgtgatcca
     4681 gaccgatgcc ggggcatcgg tgacgctcgg ttcgatgaat cagctcacca tcgatggttc
     4741 gatcatcgat catggtggca acgtcacgct cacggccgat acggcgggcg gcggttatgc
     4801 gcagctagcc aatgcgcttg cggcgtactc gagcaattcc aagtccgtct ggctcggtgc
     4861 cgacagcctg atcgatgtct ccggcatcag catcctcaat ccctacgcca gcgtcatcgc
     4921 gcagggcgcc aacctggtgc aatcgcgcac gggccggctc tacggcggcg gcaacgtgac
     4981 gatcagcaac gacacgggtt atgtcgttgc ggtcggctgc aaggagatcg cctgcgatgt
     5041 gctccagcac ggagccatca tcgatgtgcg aggcagcaac gacgcgtatg acctgcgcac
     5101 ggacagcggc ggctatgccc gcacgccggt atggagcgac ggcggcggca tcaccatcgg
     5161 cgccgctgct ggactttatt tcgatgccgc actgcatggt gacggtggtg cggcgtcagc
     5221 caatggcggc tcgctcacgg tgaccgcgga aaacgcgctg ccggttacgc ccgcgtcggg
     5281 attcggcggt gccacgcaaa tggtgctgag ccagcagagt ccggtgttca ccgccggcgc
     5341 gctgacgcca ggccagaacg tcgaagtgac caagggggtg cccgcgggca tcgtccgctt
     5401 ctccgccgac gtgctcgacg gcagcggcat caccagcctg cagcttggca gcgacccggt
     5461 gttcgaccgc acctcggcgc ccgcgccgat cacggtttcc ggcaacgtcg atcttgcgct
     5521 aggccgctcg atcgtcatca acacgacgtc gttgatcgca gcaccgctcg acgccagcgg
     5581 ctcgcacctg gatctctccg cgccctacgt cgcgttgcac ggctatgttc caggcggcga
     5641 ttatccgaag cttccggtgt tgacggcgcc ggcagccggc acgcaactgg cggtgcaggc
     5701 acaggacatc gacctgggtg gccagctctc gctgcgaaat ttcgccgatg ccagcttcgt
     5761 cagcagcgac gacctgcgct tcgtcacgcc atcgcgcgat gcgaactact tcagctccaa
     5821 cgtgctcgat ccgtcgcaga ccgtccctgg catcctggcg acagcgggca acctggaatt
     5881 ccaggccgcg cgcatttacc cggcaagcgg caatgcctac atcatcgatg ccgttggcgc
     5941 gctcactgca gggtcgggtg cgcgtgccga gaccacggtc agcttcctcg gcaacggcca
     6001 gtccgccacg gccgccacgc cgctgtccgc cggtggcagc ctgctggtcg acgccactca
     6061 catcatccag tccggcaacc tgcgtgctcc gtcaggacag atcgtgctcg gcgtcaccga
     6121 tgcgaacgac gcagtcacgc agggcttgtt cggatatacc gacagcgatc ccagcggcag
     6181 cgcgcgtttt gtcccgttgc ccctggtgtc caccgccagc gtggaccttg cagcgggcag
     6241 catcacctcc gtctcgctcg atggcctgac ggttccgtac ggccagacgg tggatggaca
     6301 gaactggcaa tacaaccccg ctgccgccgg cgtaccgggc gcgacgtcct cgctgccgga
     6361 tctgaccgcg ccgccgggca agcagatcac actgggcggt gctgccatca acatggcgag
     6421 cggttccatc gtagacctgt ccggtggcgg cgacctgcag ggccaggagt tcgtgccggg
     6481 ttcaggcggc acgcgggacg tgctgtcgca gttccagaat agtttcggca cttccgcgcc
     6541 ggttccgctc tatcccgaca gcaggatggt gtatgccgtc attcccggat accgcggact
     6601 tgccgcatac gatccttcga tcgtcgccgg ctcgccgctg gtagggcggt cggtttatct
     6661 ctcaggcggc catggattgc ctgcaggaat ttatgtgctc ctgccgggca agtacgcgac
     6721 cctgccgggc gcgttccgcg ttgtgcagga cacgcgcgtg gccgatgcgg cagctgcgca
     6781 gaacgctgtc ctggccgatg gcacccaggt gattgccggc aggttcgttg atgctttcag
     6841 cggcagcagc agcgcgcggt cgaccgcttt cctggtgcag gccgcacccg cttggcagct
     6901 gtactcgcag tacaacctga ccagcgccaa ccaatatttc tccgatttcg cgcaaagcag
     6961 cggcaccgcc atcccgctgc tggcgaccga tgccggccat ctggttctgg cggccagcag
     7021 cacgctgtcg ctgcagggca cggtcgacac ggtgcctgcg accgggggca tcggagctga
     7081 agtggacatc gcggcaacgc ggttggccat caccgcgggc ggtactgcgg cagcacccgg
     7141 gttcgtcgcc ctggatgtga atcaactcga tcaactgcag gcaagcagcc tgctcctcgg
     7201 gggtacgcgc acgcgcacag ccagcggcga cctgattacc gcgctggcga cggacgtagt
     7261 cgccagcaac ggcgacacgg cattgagcgc gcccgagatc atgctggtga gcaaggcggg
     7321 tggcggcgga atccgggtgg atgccggcag tgtcatcgcg gcccaaggca gcatcgccgc
     7381 caacgcggac gtgccgatcc agatcggcag cctgccatcg agcggcagcg gggtcagcgg
     7441 cgatggctcc ctgctgcgcg tgtccaatgg cgccgatgtc atcgtcaacc gtgtcaacgt
     7501 tcccgggatc gatgccgccg gtactgcgac cggctcgctg acgctggcgg caggcgcagt
     7561 ggtccgcgcc gaccagtcgg tcaccctgga ctccacgggc agcaccgcgg ttgaccgcac
     7621 ggcgacattc tccgcgcgca gcatcagcgc ggatgcggcg agcgtcagtt tcgtttcggg
     7681 cactgatcct gccgcgaccg gcctggtggt gggcgcggac ctgctggcgc agttcgcctc
     7741 ggccgagcag atcaccctga gcagccgcgg cgccatggac ttcatcggtg gcgtcgatct
     7801 gcaggtggcg cagaacctgg cgctgaatgc ggcggcgttc cgcaccagcg ggggtgacgt
     7861 cagcctcgat gcagcaaccc tcacgctggg caacgcgctg cacggtgcgg gttccgggtc
     7921 gaacgcggga acccatcaac tcgatctgca cgcgacgcag ctggtgtttg gcgatggctc
     7981 ggcctcgctc gacggcttcg gccaggtgaa tgcggtggca acgcgctcca ccgaactacg
     8041 cggtcaaggg cggttcgatt tcggcagcgc ggcggtcgac ctgaccacgt ccttgttgtc
     8101 ggccgctgcc ggcgccgatc acacgctggc caccacggcg gcattcagcg tgagcggtac
     8161 cccggccgct ggaaccgcgg cagccggcgg cgcgttgcgc ctgcagggcg gaagcctctc
     8221 cgtcgatacg cggctgcaaa gccttggcgg cgtcatagac ctgcacgcga gcacgggaga
     8281 tgtcgcgctt ggaagcagcg cgcaactctc ggtggccgga acggcccagc ccttttacga
     8341 cgtgacgagc tatggctctg ccggcaccat cgatctgact tccgatcagg gcagtgtcac
     8401 ggccagcgcc ggctcgctgc tcgatttcgg cagcgccagc ggcgggggca atgccgggcg
     8461 gctgaacgtg agggcgcgcc agggtgcggc catgctgaac ggcacgctgc gcggaatggg
     8521 tgccgatgcc ggcggatcgt tcggtctcga caccggcggg gccatcgatc tcaatgcgct
     8581 ggcttcgcag ctcgcagccg atggcatcaa ccagcagatc gacctgcaca gccgcagcgg
     8641 caatctccag ttgtcgacgg gacatgtgct ggctgcggcc tctgtgtcgc tcaccgctga
     8701 cggcagcgac agcagcagcg gcatggttca ggttgacgga acgattgacg cctcgggcag
     8761 cagcggcggc cagatcgccc tg
//
LOCUS       DASZZC010000236         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_920166,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000236 DASZZC010000000
VERSION     DASZZC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920166"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..542)
                     /gene="nrdR"
                     /locus_tag="VHE37_05095"
     CDS             complement(12..542)
                     /gene="nrdR"
                     /locus_tag="VHE37_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HVT34933.1"
                     /translation="MQCPFCKAPDTRVIDSRLAEEGEQIRRRRECERCGQRFTTFEKA
                     QLLMPGVIKNKTGEVEPFKEEKLRHGIESACFKRPVTPEQIDHAIDAIMRKLRHSGER
                     EVASRQIGEWVMAELSDLDHVAYVRFASIYRKFEDVNAFREEIERLQTLPTAEQRKSQ
                     LPLIEEPAAGEEKAKP"
     gene            complement(567..1850)
                     /gene="glyA"
                     /locus_tag="VHE37_05100"
     CDS             complement(567..1850)
                     /gene="glyA"
                     /locus_tag="VHE37_05100"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVT34934.1"
                     /translation="MWTHAKTLAQYDPELNAAIQAERVRQEAHIELIASENYASPAVM
                     EAQGSALTNKYAEGYPGKRYYGGCEHVDVAETLAIERVKKIFDCDYANVQPHSGAQAN
                     AAVFMALCSPGDTVMGMNLAQGGHLTHGNPANFSGKHYKIVPYGLNDAGVIDYDQMER
                     IALETRPKMLIGGFSAYSRVKDWKRMREIADKCGAMFWVDMAHIAGLVAAGEYPSPLP
                     HAHVVTSTTHKTLRGPRGGIILTKGQSEDFNKKLNSAVFPGIQGGPLMHVIAAKAVAF
                     KEALDPAFKDYQKQVVKNARAMANVLLSRGYKVVSGGTDNHLFLLDLIAKNVTGKDAE
                     AALGRAHITVNKNSVPNDPKSPFVTSGLRLGSPASTTRGFTENEMANVAGWIADIVDA
                     MSAGHNVDEAVAKVRGQVELLCARFPVYQAAKKAA"
     gene            complement(1877..>2805)
                     /gene="nadA"
                     /locus_tag="VHE37_05105"
     CDS             complement(1877..>2805)
                     /gene="nadA"
                     /locus_tag="VHE37_05105"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HVT34935.1"
                     /translation="LSQQAANSQAEYIVFCGVHFMAEVADILTFGQRKVILPDLAAGC
                     SMADMANLAKVRKCWDELSALMNPDEKVTPVTYINSAADLKAFCGNHGGIVCTSSNAR
                     AVLEWSFSRREKVLFFPDQHLGRNTGLRMGIPLEQMVTWDFSLPRGGLSEEQVRNAKI
                     ILWKGHCSVHQMFQPAHIDAFRAKYPDGKVISHPENAYEVCQKSDYVGSTEYILRTVR
                     AAKPGEHWLVGTELNLVNRLAAEMAPKGVMVQFMSPTVCMCSTMFRTDPQHLAWVLDN
                     LVAGQVVNQIKVPKEIADPARAALDLMLEVVD"
BASE COUNT          413 a          913 c          922 g          557 t
ORIGIN      
        1 accctgcgcg ctcacggctt ggctttttct tcgccggccg ccggctcttc gatcagcggc
       61 agctggctct tgcgctgctc ggcggtgggc agcgtctgca gccgctcgat ctcctcgcgg
      121 aacgcgttca cgtcctcgaa cttgcggtag atcgaggcga agcgcacgta ggccacatgg
      181 tcaagatcgg acagttcggc catgacccat tcgcctatct ggcgcgaggc cacctcgcgc
      241 tcgccgctgt ggcgcagctt gcgcatgatg gcgtcgatgg cgtgatcgat ctgctcgggc
      301 gtgaccggcc gcttgaagca ggcgctctcg atgccgtggc gcagcttctc ttccttgaac
      361 ggctccactt cgccggtctt gttcttgatc acgcccggca tcagcagctg cgctttctcg
      421 aacgtggtga agcgctggcc gcagcgctcg cactcgcggc ggcggcggat ctgctcgcct
      481 tcctcggcca ggcgcgaatc gatcacgcgc gtgtccggcg ccttgcagaa cgggcactgc
      541 atcaggggcg tccgcgcgcg gtcacgtcag gcggctttct tcgcggcctg gtaaaccggg
      601 aagcgcgcgc acagcagttc aacctgcccg cgcaccttgg ccacggcctc gtccacgttg
      661 tggccggcgc tcatggcgtc gacgatgtcg gcaatccagc cggcaacatt ggccatctcg
      721 ttctcggtga agccgcgcgt ggtcgacgcc ggcgagccca ggcgcaggcc gctggtgacg
      781 aatggcgact tcggatcgtt gggcacgctg ttcttgttga ccgtgatgtg cgcgcggccg
      841 agcgcggctt ccgcgtcctt gccggtcacg ttcttggcga tgaggtcgag caggaaaaga
      901 tggttgtcgg tgccgccgga caccaccttg tagccgcgcg aaagaagaac gttcgccatg
      961 gcgcgcgcgt tcttcaccac ctgcttctgg taatccttga acgccgggtc gagcgcttcc
     1021 ttgaaggcaa cggccttggc cgcgatcacg tgcatcagcg gaccgccctg gatgccgggg
     1081 aacaccgccg agttgagctt cttgttgaag tcctcgctct gccccttggt caggatgatg
     1141 ccgccgcgcg ggccgcgcag ggtcttgtgc gtggtgctgg tgaccacgtg cgcgtgcggc
     1201 agcgggctcg gatattcgcc ggccgcaacc agaccggcga tatgcgccat gtccacccag
     1261 aacatggcgc cgcacttgtc ggcgatctcg cgcatgcgct tccagtcctt gacgcgcgaa
     1321 taggcggaga agccgccgat cagcatcttc ggccgggtct ccagcgcgat gcgttccatc
     1381 tggtcgtagt cgatcacgcc ggcgtcgttg agcccgtacg gcacgatctt gtagtgcttg
     1441 ccggagaaat tggccggatt gccgtgcgtg aggtggccac cctgcgccag gttcatgccc
     1501 atcaccgtgt cgccgggcga gcacagcgcc atgaacaccg cagcgttggc ctgcgcaccc
     1561 gagtgcggct gcacgttggc gtaatcgcaa tcgaatattt ttttgacccg ctcgatggcc
     1621 agcgtctcgg ccacgtccac gtgctcgcag ccgccgtagt agcgcttgcc cggatagccc
     1681 tccgcgtact tgttggtcag cgcgctgccc tgtgcttcca tcaccgccgg gctggcgtaa
     1741 ttctccgaag cgatcagctc gatatgcgct tcctggcgca cgcgctcggc ctggatggcg
     1801 gcgttgagtt caggatcgta ctgggcgagg gtcttggcgt gggtccacat cgggcgcggt
     1861 ttccggttgg taggtttcag tcgacgactt cgagcatcag gtccagcgcg gcacgcgccg
     1921 ggtcggcaat ttccttcggc accttgatct ggttcaccac ctgccccgca accaggttgt
     1981 ccagcaccca ggccaggtgc tgcggatcag tgcggaacat ggtcgagcac atgcagacgg
     2041 tgggcgacat gaactgcacc atcacgccct tgggcgccat ctccgcggcg aggcggttga
     2101 ccaggttgag ctcggtgccg accagccagt gctcgccggg tttcgcggcg cgcacggtgc
     2161 gcaggatgta ctcggtgctg ccgacgtaat cgctcttctg gcacacctcg taggcgtttt
     2221 cagggtggct gatgaccttg ccgtccgggt acttcgcgcg aaacgcgtcg atgtgcgccg
     2281 gctggaacat ctggtgcacc gagcagtggc ccttccacag gatgatcttc gcgttgcgga
     2341 cttgttcttc gctgagcccg ccgcgcggca gcgagaagtc ccaggtgacc atctgttcca
     2401 gcgggatccc catgcgcagg ccggtgttgc ggcccaggtg ctggtcgggg aagaacagca
     2461 ccttctcgcg gcgcgagaag ctccattcca gcaccgcgcg cgcgttgctc gaggtgcaga
     2521 cgatgccgcc gtggttgccg cagaacgcct tcaggtcggc ggcggaattg atgtaggtga
     2581 ccggcgtgac cttctcgtcc gggttcatca gcgccgagag ttcgtcccag cacttgcgca
     2641 ccttggccag gttggccatg tcggccatcg agcagccggc ggccaggtcg ggcaggatca
     2701 ccttgcgctg gccgaaggtg aggatatccg ccacctcggc catgaaatgc acgccgcaga
     2761 acacgatgta ctcggcctgg ctgttggccg cctgctgcga cagct
//
LOCUS       DASZZC010000237         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_149748,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000237 DASZZC010000000
VERSION     DASZZC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149748"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1020)
                     /locus_tag="VHE37_05110"
     CDS             complement(259..1020)
                     /locus_tag="VHE37_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HVT34936.1"
                     /translation="MTVGLLIVGDELLSGKRQDQHLPRVISMLAERGMALDWAQFVGD
                     CEERIAAAIRAADARGDVVLSCGGIGATPDDLTRQAAARAFGVPLQRHAEGEALIVQQ
                     YGDQAFPNRVLMADFPAGAGLIPNPVNRVAGFYFRDHNFVPGFPEMAWPMLQWVLDTR
                     YPQLRNADPPVEYSLRAVGTSSEGDLLPLMEKVLADFPGVTLSSLPFRGDATRPRHIE
                     FGIKGPRDLAAAAYRSLRAQLAAWPAMALVELRAP"
     gene            complement(1056..>2084)
                     /locus_tag="VHE37_05115"
     CDS             complement(1056..>2084)
                     /locus_tag="VHE37_05115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT34937.1"
                     /translation="DGAASWRPAPQSAATLLLLNVAMLLGGSSRRSERFDVGDLVTLL
                     GTLLPFAVLLGYLLDATELYQGGSLGGGASPMAALLLLVLAFGQISLYPRRGLLSAFT
                     TPTAGAAVGRRLLPWVMLLPVLFGCMQVWVVKVELMTLPLAVASTVTISIIAFIYLIY
                     RVSNLMTQLEAQQAEEHVQRESAAKEEGMTDALTGLLNRRGWEKHLRLEEERCKRHGH
                     NGCIVMIDLDDLKKVNDTQGHSQGDELIKRAGQALLSGARRGDVLARLGGDEFAYLAI
                     GCQPEHAGVVVRRLAEALMNAKVGASLGYALRDINVNLQGAFDEADRSMYANKRARKA
                     KRVAEAAN"
BASE COUNT          351 a          719 c          665 g          349 t
ORIGIN      
        1 ccacctgcag ctggtggttg ggcacgttgt cgcgcaaaac ctcctgggcc aggcgcaggt
       61 cagcggggct gtcctcaacc agcatgatca ccgggttggg agccttgggc tttgctgatg
      121 cggtcatgca cgctcttgaa cgatcggttt gcggaacccg cgcaatgtag caactattgc
      181 atcaactgcc acgggatatt tagtcacggc cggcccgctc atcgctcgcc ccgccccttc
      241 cgtcttgtcg cgcggtgctc acggtgcccg cagttcaacc agcgccatgg ccggccatgc
      301 ggcgagttgc gcgcgcaggc tccggtaggc cgccgcggcc aggtcgcgcg gccccttgat
      361 gccgaactcg atgtggcgcg ggcgtgttgc gtcgccacga aacggcaggc tggacaaggt
      421 cacacccgga aaatccgcca gcaccttttc catcagcggc agcaggtcac cttccgaact
      481 ggtgccgacg gcgcgcagcg aatattccac cggcggatcg gcattgcgca gctgcggata
      541 acgcgtatcc agcacccact gcagcatggg ccaggccatc tcgggaaatc ccggcacgaa
      601 gttgtgatcg cggaaataaa atccggccac gcggttcacc gggttgggaa tcaggccggc
      661 accggccgga aaatcggcca tcaacacgcg gttgggaaaa gcctggtcgc cgtactgctg
      721 cacgatcagc gcttcgccct cggcatgccg ctgcaagggc acaccgaaag cccgcgcggc
      781 cgcctgccgg gtcagatcgt ccggtgtcgc accgatgccg ccgcacgaca gcacgacatc
      841 gccgcgcgcg tcggctgcac gtatcgccgc tgcgatgcgt tcctcgcagt cgccgacgaa
      901 ctgggcccag tccagtgcca tgccgcgctc ggccagcatg gagatgaccc tgggcagatg
      961 ctggtcctgg cgcttgccgg acagcagttc gtccccgacg atcagcaggc cgacggtcat
     1021 ggttccaggg aattgcggtt cgcgcgcggc gctgctcaat tcgccgcttc ggccacgcgc
     1081 ttggccttgc gcgcacgctt gttggcatac atggagcgat cggcttcgtc gaaagcgccc
     1141 tgcaggttga cgttgatgtc gcgcagcgca tagccgagcg atgcgccgac cttggcattc
     1201 atcagcgctt cggccagccg ccgcaccacc acgcctgcat gctccggctg gcagccgatg
     1261 gccaggtagg cgaattcatc gccgccgaga cgcgcgagca cgtcgccccg gcgcgcgccg
     1321 ctgagcagcg cctgccccgc gcgcttgatc agttcatcgc cctggctgtg accctgtgta
     1381 tcgttgacct ttttcagatc atccaggtcg atcatcacga tgcaaccgtt gtgcccgtgg
     1441 cgcttgcagc gttcctcttc cagccgcagg tgcttttccc agccgcggcg gttgagcagc
     1501 ccggtgagcg catcggtcat gccttcttcc ttggccgcac tctcgcgctg cacgtgctcc
     1561 tcggcctgct gcgcttccag ctgcgtcatc aggttgctga cgcggtagat caggtagatg
     1621 aaggcgatga tgctgatggt gaccgtgctg gccaccgcca gcggcagcgt catcagctcg
     1681 accttcacca cccagacctg catgcagcca aacagcacgg gcaggagcat gacccatggc
     1741 agcaggcggc ggccgacggc cgcgccggcg gtaggggtgg tgaacgcgga cagcaggcct
     1801 cgccgcgggt acaggctgat ctggccgaaa gccagtacca gcagcagcag cgccgccatc
     1861 ggtgatgcgc cgccgccgag actgccgccc tggtacagct cggtggcatc cagcaggtag
     1921 cccagcagca cggcgaacgg cagcagcgta ccgagcagcg tcaccaggtc accgacatcg
     1981 aagcgttccg acctgcgcga gcttccaccc agcagcatgg ccacgttgag caacagcagg
     2041 gtggcggcac tctgcggcgc cggccgccag gatgccgcgc catc
//
LOCUS       DASZZC010000238         5692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_533112,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000238 DASZZC010000000
VERSION     DASZZC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533112"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="dprA"
                     /locus_tag="VHE37_05120"
     CDS             <1..859
                     /gene="dprA"
                     /locus_tag="VHE37_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HVT34938.1"
                     /translation="DPRYPATLRGIAQAPAALFYLGDADVLAQPQLAIVGARSATPQG
                     VEDARAFAADLAGRGMVIGSGLALGIDAAAHRGALEAGGYTVAVCATGLDRVYPARHR
                     ELAHRIAASGVLISEFPPGVQAIAANFPKRNRLISGLALGVLVVEAALESGSLITARL
                     AVEQGREVFAIPGSIHNPMARGCHALIRQGAKLVERADDILEELGPRVSAYLREHGSS
                     AAADAPAADAAEERILAALGHGSLSFDQLMEGAQLPHQEFHAALLRLELRGALAAVAG
                     GGYMRVFGR"
     gene            1088..1555
                     /locus_tag="VHE37_05125"
     CDS             1088..1555
                     /locus_tag="VHE37_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HVT34939.1"
                     /translation="MKETVLDVLMYLFENYQTGEFSDTDNQETLRDELIAAGFPEEEV
                     GHAFAWLDGLAQQRQLPLVFGPAHSIRVYAAEEQARLSPECRGFLLYLEQLGLLTLQA
                     RELVIERLLALKEEIDLERVKWVCLLVLINTPASEEAFSCVEEMVYCSSEFLH"
     gene            1676..4093
                     /locus_tag="VHE37_05130"
     CDS             1676..4093
                     /locus_tag="VHE37_05130"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HVT34940.1"
                     /translation="MSKNLVIVESPAKAKTIKKYLGHDYEVLASFGHVRDLVPKDGAV
                     DVKNGFAMKYQAIDKNEKHVRAIASALKSADALFLATDPDREGEAISWHLLELLRERN
                     ALKGKDVKRVVFYEITQREVQKAIAEPRDVAMDLVNAQQARRALDYLVGFNLSPLLWR
                     KVQTGLSAGRVQSPALRLICERELEIRAFVPKEYWTLDARAQKGEQGFSARLLELDGK
                     KAEQFTLTDAGGSEAAKQRILDAARGELLVANVDKKQRRRNPAPPFTTSTLQQEANRK
                     LGFTSSRTMRTAQQLYEGVDIGGDTVGLITYMRTDSVNLAQDALTEMRGVIQQQFGAA
                     ALPPEPRYFKAKSKNAQEAHEAVRPTSSARLPKQVVAYLTPDQAKLYELIWKRAVASQ
                     MTQAVFDTVAVELRAGEQHAFKANGSTLIEPGYLAVYNVGHDAGDAADDDDDKRLPPL
                     EAGEKVKLLELLPEQHFTEPPPRYTEASLVKALEEYDIGRPSTYASIISTLTGRDYVR
                     LDNKAFTPTDVGMVVNKFLTEHFTRYVDYEFTAHLEDDLDAVSRGEKQWVPLLAEFWR
                     DFKPRVDEKMELKREEVSSARQIGTDPVSGKPVSARIGRFGPFVQIGTKDDADKPRFA
                     SLKPNQRIDTLTLQDALDLFKLPRMLGHTPEGEEVAANIGRFGPYVRYGSQFVSMKKE
                     DDPFTIELPRALELIAQKAAALDAATLKNFEGTGIRIIKGKFGPYIADGPRKARIPKG
                     KDYDKLSVFECEAYLAEAEAAKKPVRKKKAAAPAAEAKPAPAAKKAARKTAKKTAAKG
                     RARKQAG"
     gene            4090..4644
                     /locus_tag="VHE37_05135"
     CDS             4090..4644
                     /locus_tag="VHE37_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HVT34941.1"
                     /translation="MNPAQLRAAVAALHAGQVIAYPTEAVWGLGCDPENQLACERIFA
                     LKQRDWRKGLILVGSDFAQFEPYIRMPSNSALKRATATWPGPHTWIFPCADDAPMWLT
                     GERDDIALRVSAHPVVRALCERFGGALVSTSANRSGREPARSATQLRLQFGPLTPRVV
                     PGAVGGMVKPTPIRNVITGAIIRA"
     gene            4641..5585
                     /gene="hemF"
                     /locus_tag="VHE37_05140"
     CDS             4641..5585
                     /gene="hemF"
                     /locus_tag="VHE37_05140"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HVT34942.1"
                     /translation="MSVFETNTDAVVAYLKQLQDRYCAAFEAADRHAKFREDAWDRVP
                     TGDPKQGITGGGVTRVLSDGALFERAGVNFSLVEGRKLPPSASASRPQLIGRSFKAMG
                     VSVVLHPKNPYVPTAHCNVRLFVAEKIGEKPVWWFGGGYDLTPYYGFEEDAREWHRAA
                     RDACAPHGDDIYARLKKSCDEYFVLKHRGEQRGVGGVFFDDWSEGGFDKSFALMRSIG
                     DSFPAAYLTIARRRIASPYGAREREFQLYRRGRYVEFNLVWDRGTLFGLQSGGRTESI
                     LMSMPPGAIWKYDWKPEPDSPEADLYERFLKPRDWLGS"
BASE COUNT         1019 a         1914 c         1875 g          884 t
ORIGIN      
        1 cgacccacgc tatcccgcta cgctgcgcgg catcgcgcag gcgccggccg cgctgttcta
       61 tctcggtgac gccgatgtgc tggcgcagcc gcagctggcc atcgtcggcg cgcgcagcgc
      121 cacgccgcag ggcgtcgagg acgcgcgcgc cttcgccgcc gacctggccg ggcgcgggat
      181 ggtgatcggc agcgggctgg cgctgggcat cgacgccgcc gcgcatcgcg gcgcgctcga
      241 agccggcggc tacaccgtgg cggtctgcgc caccgggctc gaccgcgtct atcccgcgcg
      301 tcaccgcgaa ctggcgcacc gcatcgcggc gtccggcgtg ctgatctccg aattccctcc
      361 cggcgtgcag gccattgccg ccaacttccc caagcgcaac cggctgatca gcgggctggc
      421 gctgggcgtg ctggtggtgg aggccgcgct ggaatcggga tcgctgatca ccgcgcggct
      481 ggcggtggag cagggtcgcg aggtgttcgc cattcccggt tcgatccaca acccgatggc
      541 gcgcggctgt cacgcgctga tccgccaggg cgccaagctg gtggagcgcg ccgatgacat
      601 tttggaagaa ctcggaccgc gcgtgagcgc ctacctgcgc gagcacggca gcagcgcggc
      661 ggccgatgcg cccgcggccg atgccgccga ggaaagaatt cttgccgcgc tcggtcatgg
      721 cagcctgtcg tttgaccagc tgatggaggg cgcgcagctg ccgcaccagg agttccacgc
      781 cgcgctgctg cggctggaac tgcgcggcgc actggccgcg gtggcgggcg gcggctacat
      841 gcgggttttc ggccgctgat gccccgcaaa aagatgccac cgcatcggcc gtagtgatgt
      901 aatttattat cgaatatatt taaatatatt atataattaa taacaacttt gacacgggtg
      961 cggtgggtgt atattttgtg gcttgtgcgg caaaagtcag caaaaaaaca cattcccggc
     1021 atcagccgga tcttgcctcg cgcgcggcgt ctgtattcca tgcggcggtc cttccaagga
     1081 gtgttccatg aaggaaacgg tgctcgacgt cctgatgtac ctgttcgaga actaccagac
     1141 gggggagttc tctgacaccg acaaccagga gacgctgcgc gacgagctga tcgccgcggg
     1201 ctttcccgaa gaggaagtcg gccacgcgtt tgcctggctc gacggcctgg cgcagcagcg
     1261 ccagctgccg ctggtgtttg gccccgcgca ttccatccgc gtgtacgccg ccgaagagca
     1321 ggcgcgcttg tcgcccgagt gccgcggctt cctgttgtac ctcgagcagc tgggcctgct
     1381 cacgctgcag gcgcgcgaac tggtgatcga gcgcctgctc gcactcaagg aagagatcga
     1441 cctggagcgc gtgaagtggg tctgcctgct ggtgctgatc aacacgccgg ccagcgaaga
     1501 ggccttctcc tgcgtcgagg aaatggtcta ctgcagcagc gagttcctgc actgacgcaa
     1561 ccccgcgcgc ggcggccgcc ggcgcccgcg atactggact ttttgcgccg cttccgcgta
     1621 taagccgcgc tcaacgggcg ctgcatgcgc catttttttc cccgcctgag cgttgatgag
     1681 caagaacctg gtcatcgtcg aatcgccggc caaggcgaag acgatcaaga aatacctggg
     1741 acacgactac gaggtgctcg cctcgttcgg tcacgtgcgc gacctggttc ccaaggacgg
     1801 cgcggtcgac gtgaagaacg gtttcgccat gaagtaccag gcgatcgaca agaatgaaaa
     1861 gcacgtgcgc gccatcgcca gcgcgctgaa gtccgccgat gcgctgttcc tggccaccga
     1921 cccggaccgc gagggcgaag ccatttcctg gcaccttctg gaactgctgc gcgagcgcaa
     1981 cgcgctcaag ggcaaggacg tcaaacgcgt ggtgttctac gagatcaccc agcgcgaagt
     2041 gcagaaggcc atcgccgaac cgcgcgacgt ggccatggac ctggtcaatg cgcagcaggc
     2101 gcgtcgcgcg ctggattacc tggtcggctt caacctgtcg ccgctgctgt ggcgcaaggt
     2161 gcagaccgga ttgtccgccg gccgcgtgca atcgccggca ctgcgcctga tctgcgagcg
     2221 cgaactggaa atccgcgcct tcgtgccgaa ggaatactgg acgctggacg cgcgcgcaca
     2281 gaagggcgaa cagggcttca gtgctcgcct gctggaactc gatggcaaga aggccgagca
     2341 gttcacgctg accgatgccg gcggctcgga ggcagcgaag cagcgcatcc ttgatgccgc
     2401 gcgcggcgaa ctgctggtgg ccaacgtcga caagaagcag cgccggcgca acccggcgcc
     2461 gccgttcacc acctcgacgc tgcaacagga agccaaccgc aagctcggct tcacctccag
     2521 ccgcaccatg cgcaccgcgc agcagctgta tgaaggcgtg gatatcggcg gcgacaccgt
     2581 cggcctcatc acctacatgc gtaccgactc ggtcaacctg gcgcaggacg cgctgactga
     2641 aatgcgcggc gtgatccagc agcagttcgg cgccgccgcg ctgccgccgg aaccgcgcta
     2701 tttcaaggcc aagagcaaga acgcgcagga agcgcacgag gcggtgcgcc cgacctcgtc
     2761 ggcgcgcctg cccaagcagg tggttgccta tctcacgcca gaccaggcca agctgtacga
     2821 actgatctgg aagcgcgccg tggcctcgca gatgacgcag gcggtgttcg ataccgtcgc
     2881 tgtcgaactg cgcgccggcg agcagcacgc gttcaaggcc aacggctcca cgctgatcga
     2941 gccgggttac ctcgccgtgt acaacgtcgg ccacgacgcc ggtgatgcgg ccgacgatga
     3001 cgacgacaag cgcctgccgc cgctggaagc gggcgagaag gtcaagctgc tcgaactgct
     3061 gcccgagcag catttcaccg agccgccgcc gcgctacacc gaggcctcgc tggtcaaggc
     3121 gctggaggaa tacgacatcg gccgcccgtc gacttacgcc agcatcatct ccacgctcac
     3181 cggccgcgat tacgtgcgcc tggacaacaa ggccttcacg cccaccgacg tcggcatggt
     3241 ggtgaacaag ttcctcaccg agcacttcac ccgctacgtc gactacgaat tcaccgccca
     3301 tctcgaggac gatctcgacg ccgtgtcgcg cggcgagaag cagtgggtgc cgctgctggc
     3361 cgagttctgg cgcgacttca agccgcgcgt cgacgagaag atggaactca agcgcgagga
     3421 ggtttcctcc gcgcgccaga tcggcaccga cccggtgtcg ggcaagccgg tgtcggcgcg
     3481 catcggccgc ttcggcccgt tcgtgcagat cggcaccaag gacgacgccg acaagccgcg
     3541 cttcgcctcg ctcaagccga accagcgcat cgacacgctg acgctgcagg acgcactgga
     3601 cctgttcaag ctgccgcgca tgctcgggca cacgccggag ggcgaggaag tggccgccaa
     3661 tatcggccgc ttcggtccct atgtgcgcta cggcagccag ttcgtctcga tgaagaagga
     3721 agacgatccg ttcaccatcg aacttccgcg cgcgctggaa ctgatcgcgc agaaggctgc
     3781 ggcgctggat gccgccacgc tgaagaattt cgaaggcacc ggcatccgca tcatcaaggg
     3841 caagttcgga ccctacatcg ccgacggccc gcgcaaggcg cgcatcccca agggcaagga
     3901 ttacgacaag ctgtcggtgt tcgagtgcga ggcctacctc gccgaagccg aagcggcgaa
     3961 gaaaccggtg cgcaagaaaa aggccgcagc gccggccgcc gaagccaagc ccgcgccggc
     4021 ggcgaagaaa gcggccagga agaccgccaa gaaaactgcc gccaagggac gggcgcgcaa
     4081 gcaggccgga tgaacccggc acaactgcgc gccgcggtgg cggcgctgca cgccggccag
     4141 gtcatcgcct atcccaccga ggcggtatgg ggcctgggct gcgacccgga aaaccagttg
     4201 gcgtgcgagc gcatcttcgc gctcaagcag cgcgactggc gcaagggcct gatcctggtc
     4261 ggcagcgact tcgcgcagtt cgagccgtat atccgcatgc cgagcaacag cgcgctcaag
     4321 cgcgccaccg ccacctggcc gggaccgcac acctggattt tcccctgcgc cgacgacgcg
     4381 ccgatgtggc tgaccggcga gcgcgatgac attgcgctgc gcgtgtcggc gcacccggtg
     4441 gtgcgtgcgc tgtgtgaaag attcggcggc gcgctggtct ccaccagcgc caaccgctcc
     4501 ggccgcgagc ccgcgcgcag cgccacccag ctgcgcctgc agttcggacc cctcactccc
     4561 cgggtggtgc cgggcgcggt gggcggcatg gtaaaaccta ctcctatccg caacgtgatc
     4621 acgggggcga tcatccgcgc atgagtgtat tcgagaccaa tactgacgcc gtcgtcgcct
     4681 acctcaagca gctgcaggac cgttactgcg cggcgttcga ggcggccgac cgccacgcca
     4741 agttccgcga agacgcctgg gaccgcgtgc ccaccggcga tcccaagcag ggcatcaccg
     4801 gcggcggcgt cacgcgcgtg ctgtccgacg gcgcgttgtt cgaacgcgcc ggcgtgaatt
     4861 tctcgctggt ggaaggacgc aagctgccgc cctcggccag cgccagccgt ccgcagctga
     4921 tcggacgcag cttcaaggcc atgggcgtgt cggtggtgct gcatccgaag aatccctacg
     4981 tgcccactgc gcactgcaac gtgcgcctgt tcgtggccga gaagatcggc gagaagccgg
     5041 tatggtggtt cggcggcggc tacgatctca ccccgtacta cggcttcgag gaagacgcgc
     5101 gcgagtggca ccgcgcagcg cgcgatgcct gcgcgccgca tggcgacgac atctacgcgc
     5161 gcctgaagaa atcgtgcgac gaatacttcg tgctcaagca ccgcggcgag cagcgcggcg
     5221 tcggcggcgt gttcttcgac gactggagcg aaggcggctt cgacaagagc ttcgcgctga
     5281 tgcgcagcat cggcgacagc ttcccggccg cgtacctgac cattgcgcgc cggcgcatcg
     5341 ccagccccta cggcgcgcgc gagcgcgagt tccagctgta ccggcgcggc cgctacgttg
     5401 agttcaacct ggtgtgggac cgcggcacac tgttcggcct gcagtccggc gggcgcaccg
     5461 aatccatcct gatgtcgatg ccgccgggtg cgatctggaa atacgactgg aagcctgaac
     5521 ccgattcccc ggaagccgat ctctacgagc gcttcctcaa gccgcgcgac tggctgggca
     5581 gctaggtcgc gttttgcagg agcgggcctt gcccgcgatt attttctcgc gcgagtcgcg
     5641 cgcaaggcac gctcctgcat gatttgctca gcgccggcgc accgcgcgca at
//
LOCUS       DASZZC010000239         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_211815,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000239 DASZZC010000000
VERSION     DASZZC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211815"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VHE37_05145"
     CDS             <1..1135
                     /locus_tag="VHE37_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34943.1"
                     /translation="GNCLRGGNTATAIREADGPGGSGINVALGDGGAEGIFALHEADG
                     RIASIVTGEGTSISNSRLRRYDADLANGVDLLNLTNPDIRTSVAHEVWGRCLYLVATT
                     NKTGVPSLVRFAADGSAHKLHPLNNHWSNTLATDADNVYFADNNDVLRLRHGQNLASA
                     AVTVTTLPNSDSVIDMGIAGDGSLYLSGFNLGSGVKHTGTYTVASAASAAVPLQILPA
                     TSGVIASDGDLLYADDYDSSTGTRTAITVKTDGTVVSSLPNASYSPLTPGAFNADGAG
                     LDPLLRHYIQPAKLLLLEARSPDTAVSVVDLATGHVLKAGALPTTGGSLGSARGFGRR
                     VMLAWYSNASGASSNYDLFALDTSTSNSIAAFQNTAGDERTFP"
     gene            1226..2131
                     /locus_tag="VHE37_05150"
     CDS             1226..2131
                     /locus_tag="VHE37_05150"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVT34944.1"
                     /translation="MKKQWTAADMPPQDDKLALVTGANRGLGLEISVGLARAGARVVM
                     ACRDAQRAQDARAEVQRRVPNAQVELMSLDLADLASVRAFAQAYAAKFPRIDILCNNA
                     SAIMVPLSKTRDGIEMHMGVNHFGHFALTGLLLEQLKAAPRARIVNTASLAHRMTPGL
                     DLDDLGWERKPYNVMHAYGQSKLASLVYTFELGRRLRKSGSTIVAAAAHPGYTATNPD
                     LGGFFMRLSTRLFAQAPAMGALPALYAATMPDVTDGDYLGPGGMKELGGAPKKVRARE
                     EAYDGALGARLWALSEQLTGVRYLD"
     gene            complement(2128..2340)
                     /locus_tag="VHE37_05155"
     CDS             complement(2128..2340)
                     /locus_tag="VHE37_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34945.1"
                     /translation="MTRKRRRVMYAGKPVKPQGEWPMLEPMPLDDEAIAAGVAVDALG
                     SVPARPLDGTPDDLTPSTLLGEHRPG"
BASE COUNT          456 a          816 c          775 g          383 t
ORIGIN      
        1 gggaaactgc ctgcgtggcg gtaacaccgc gacggccatc cgcgaggcgg acgggccagg
       61 gggcagcggc atcaatgttg cgctgggtga cggtggtgca gaaggaatat ttgcgttgca
      121 tgaagccgac ggccgtatcg ccagcatcgt caccggcgaa gggaccagca tctccaactc
      181 gcgcctgcga cgctacgatg ccgatctggc aaacggcgtc gacctgctca acctgaccaa
      241 tcctgacatc cgcaccagcg tcgctcatga agtctggggc cgctgcctgt acctggttgc
      301 aaccaccaac aagaccggcg tgccatcgct agtgcgattt gccgctgacg gcagcgcgca
      361 caagctgcac ccgctcaaca atcactggag caatacgctg gcgaccgacg ccgacaatgt
      421 ctactttgcc gacaacaacg acgtgctgcg cctgcggcat gggcaaaacc tggcctccgc
      481 cgccgtgacc gtcaccacac tgcccaacag cgacagcgtg attgacatgg gcattgccgg
      541 agatggcagc ctgtaccttt ccggcttcaa cctgggctcg ggcgtcaagc acaccgggac
      601 ctacaccgtg gcttcggccg caagcgcggc ggtgccgctg cagatcctgc cggcgacgag
      661 cggggtcatc gcaagtgatg gcgatttgct ctacgccgac gattacgatt cttcgactgg
      721 aaccaggacc gcgatcacgg taaagaccga tggcactgtc gtcagcagcc tgcccaatgc
      781 aagctactct ccattgaccc ccggagcgtt caatgcagat ggcgcgggac tcgatccgct
      841 gctgcgccac tacatccaac ccgccaagct gctcctgctg gaagcaagat ctcccgacac
      901 cgccgtcagc gtggttgacc tggcaacggg ccatgtgctg aaagcgggcg cgctgccaac
      961 gaccggaggc tcgctcggct cggcgcgagg attcggaaga cgcgtcatgc tcgcctggta
     1021 cagcaatgcg tcgggtgcca gttccaacta cgatctcttt gccctcgata cttcaacatc
     1081 gaactccatt gccgcgtttc agaatacggc gggtgatgaa cgtacttttc cctgaagcga
     1141 aaagtcggac cagaattcgc aacgcttctc cggcctggag ataagcacga acaagacagt
     1201 ttcaaggact tcaaagggag caagaatgaa aaaacaatgg accgcggccg acatgccgcc
     1261 gcaggacgac aagctggcgc tggtcaccgg tgccaaccgc gggctggggc tggagatcag
     1321 cgtcggcctt gcgcgcgccg gcgcgcgcgt ggtcatggcc tgccgcgacg cgcagcgggc
     1381 gcaggacgcc cgcgccgaag tgcagcggcg tgtgcccaac gcgcaggtcg agctgatgtc
     1441 gctggatctc gccgacctgg cctcggtgcg cgctttcgcg caggcctacg cggcgaagtt
     1501 tccgcgcatc gacatcctgt gcaacaacgc cagcgccatc atggtgccgc tgtccaagac
     1561 cagggacggc atcgagatgc acatgggcgt gaaccacttc ggccacttcg cgctcaccgg
     1621 cctgctgctg gagcaactga aggccgcacc gcgcgcgcgc atcgttaaca ccgcgagcct
     1681 ggcgcaccgg atgacgcccg gactcgatct cgacgatctc ggctgggagc gcaagccgta
     1741 taacgtgatg cacgcctacg gccagagcaa gctggcctcg ctggtgtaca ccttcgaact
     1801 cgggcgccgg ctgcggaagt cgggctccac catcgtcgcg gcggccgcgc acccgggcta
     1861 caccgccacc aatcccgatc tcggcggatt cttcatgcgg ctgtcgacgc gcctgtttgc
     1921 gcaggccccg gcgatgggcg cactgcccgc gctgtacgcg gcaaccatgc ccgacgtgac
     1981 cgacggcgat tacctcggac ccggcggcat gaaggaactc ggcggcgcgc cgaagaaggt
     2041 gcgggcgcgc gaggaagcgt acgacggcgc gctcggcgca aggctgtggg cgctgtcgga
     2101 acagctgacc ggcgtgcgct atctcgacta gccggggcgg tgttcgccga gcagggttga
     2161 cggcgtgaga tcgtccggcg tgccgtcgag cggccgcgcc gggacgctgc cgagcgcatc
     2221 gaccgcgaca ccggcggcga tggcctcgtc atccagcggc atcggctcga gcatgggcca
     2281 ttcgccctgc ggcttcaccg gcttgccggc atacatcacc cgccgtcgct tcctggtcat
     2341 ggcgcgctcc ttcgcttgcc ctgctagaca gaccgccgcg cgcaaaaaaa gattcccgcc
     2401 gcacgcgggg cgcacggcgg gaaccggtga
//
LOCUS       DASZZC010000240         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1044153,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000240 DASZZC010000000
VERSION     DASZZC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044153"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VHE37_05160"
     CDS             complement(<1..629)
                     /locus_tag="VHE37_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HVT34946.1"
                     /translation="MSGPSHVAEQPIERRAQLVDYLAAGGRPAADWKIGTEHEKFVFR
                     TDDLKPPPYEGERGIGALLRGIECCGWEPVEENGNIIALARGNASVTLEPAGQLELSG
                     APLETIHQTCCEVQEHLDCVRRAAEPLQLGILGMGFQPKWKRDEMPWMPKGRYAIMRR
                     YMPKVGSLGLDMMTRTCTVQVNLDFADEADMVKKFRVSLALQPIATALFA"
     gene            complement(709..1338)
                     /gene="yihA"
                     /locus_tag="VHE37_05165"
     CDS             complement(709..1338)
                     /gene="yihA"
                     /locus_tag="VHE37_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HVT34947.1"
                     /translation="MAGTQAQGRKNTANNPLRGAAFVLAAHRVDQLPADTGAEVAFAG
                     RSNAGKSSAINALADQGALARTSKTPGRTQQLVVFAMPDGRRLVDLPGYGYAKVPPSL
                     RQHWKQEIDAYLRNRQSLRGLVLIADIRHAPAAFDRQMLAFCAAIELPCLMLLSKADK
                     LSRAQARTRQREVEAEIAEEGGRVQLIPFSATAGTGVPEARAAVAALLA"
     gene            1450..2289
                     /locus_tag="VHE37_05170"
     CDS             1450..2289
                     /locus_tag="VHE37_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT34948.1"
                     /translation="MGFRRIAAVLVMLAATAPIAVALSAPPAKYKPAASKPAASAPAA
                     SAPASSSSAAAPASAASAGGAAKAAEAEFAKLEAVATKPGDATAGQAKAAACGACHGM
                     DGNSSDAQYPKLAGQSEQYIATQLMRFKSGTRQNAIMQGMASTLAPQDMHDIGAYFSQ
                     QKRLPGVADAKLASAGGKLYREGDSSRGIPACMACHGPDGAGNPGWRVPNIGGQHADY
                     IQAQLKAWHDGTTWGSDTHAMIMPSIAQHLTEQDISAVSSYVEGLHPAAAPKANPKSD
                     AID"
     gene            2362..3060
                     /locus_tag="VHE37_05175"
     CDS             2362..3060
                     /locus_tag="VHE37_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HVT34949.1"
                     /translation="MIKRTVVFLLLAATAGLASAQAPQQWTEGKDYFAIQNPQPTDQP
                     DKVVVTEVFSFACPACNQFEPYLDKLRAELPAGAVLEFLPASWHANEDWPVFQRAYFT
                     AKALGIDNKQSHDAVFKAIWGPNGPLATYDPTTQRPKAEANLPKLEDVAKFYAQFGAK
                     PADFIATADSFTVNLQMKRADQQIMAWGTDSTPTLVIDGKWRLTPASTKGAQQAVDLT
                     LYLVNKELAAKKAK"
     gene            3062..>3319
                     /locus_tag="VHE37_05180"
     CDS             3062..>3319
                     /locus_tag="VHE37_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34950.1"
                     /translation="MNASRLLRASLLICGLVLTAACSAHNDAGDVSAPSTAATPAPAV
                     TANFTEGKEYVKLAPPQGTPAPTGAVEVVEVFSYGCPHCAEF"
BASE COUNT          568 a         1154 c         1087 g          510 t
ORIGIN      
        1 gcgaacagcg cggtcgcgat cggctgcagc gccagcgaca cgcggaattt cttcaccatg
       61 tcggcttcgt cggcgaagtc gaggttgact tgcacggtgc aggtgcgggt catcatgtcg
      121 agtccgagcg agccgacctt cggcatgtag cggcgcatga tcgcgtagcg gcccttgggc
      181 atccacggca tctcgtcgcg cttccacttc ggctggaagc ccatgccgag gattccgagc
      241 tgcagcggtt cggccgcacg gcgcacgcaa tccaggtgtt cctgcacttc gcaacaggtc
      301 tggtggatcg tctcgagcgg cgcgccggac aattccagtt ggccggcggg ctccagcgtc
      361 accgacgcgt tgccgcgcgc cagcgcgatg atgttgccgt tttcctcgac gggctcccag
      421 ccgcagcact cgatgccgcg cagcagcgcg ccgatgccgc gttcgccttc gtacggcggc
      481 ggcttcaggt cgtcggtgcg gaacacgaac ttctcgtgct cggtgccgat cttccagtcc
      541 gccgcgggac gaccgcccgc cgcgagatag tccacgagtt gcgcgcggcg ttcgatcggt
      601 tgttcggcaa cgtggctggg accggacatg cgctgcttcc gcctgggaca aggatgccaa
      661 gatggggcgg cggacgccga tccgcaagtg ggcgggaagc ggaccgcgct aggcgagcag
      721 cgctgccacc gccgcgcgcg cttccggcac gccggttccg gccgtggccg agaacggaat
      781 caattgcacc cgcccaccct cttctgcgat ttcggcttcg acctcgcgct ggcgggtgcg
      841 cgcctgcgcg cgtgacagct tgtcggcctt gctgagcagc atcaggcacg gcagttcgat
      901 ggccgcgcag aacgccagca tctgccggtc gaacgcagcc ggcgcgtggc ggatatcggc
      961 gatcagcacc agcccacgca gcgattgcct attgcgcagg tacgcgtcga tctcctgttt
     1021 ccagtgctgg cgcaggctcg gcggcacctt ggcgtaaccg taacccggca ggtcgacgag
     1081 ccggcggccg tccggcatcg cgaacaccac cagttgctgg gtgcggcccg gcgtcttgct
     1141 ggtgcgcgcc agcgcgccct ggtcggcgag cgcattgatg gcgctggact tgccggcgtt
     1201 ggaacgtccc gcgaaagcga cctcggcgcc ggtgtccgcc ggaagctggt cgacgcgatg
     1261 tgcggccagc acgaacgccg cgccacgcaa tgggttgttc gctgtgtttt tacgtccctg
     1321 tgcctgggtc ccggccattc cctggccggg atgacgtgtt tgacgcatgt ttagccccgc
     1381 ggtgataatc ccgcggttcg cgccggctcg ttgcaccggc gctcaacatc caacgggaga
     1441 gaggattcta tgggtttccg gcgcatcgca gctgtccttg tcatgctcgc cgccacggcg
     1501 ccgatcgcgg tggcgctgtc ggcgccgccg gcgaaataca aaccggctgc gtcgaaaccc
     1561 gcagcatctg cgcccgccgc ctccgcgccc gccagcagtt cgagcgccgc cgcaccggcc
     1621 agcgccgcaa gcgcgggcgg tgccgcgaag gctgccgaag ccgaattcgc gaaactcgaa
     1681 gccgtcgcga ccaaacccgg cgacgcgacc gccggccagg ccaaggccgc cgcgtgcggc
     1741 gcctgccatg gcatggacgg caattccagc gacgcgcaat atcccaagct cgcgggccag
     1801 agcgagcaat acatcgccac ccagctgatg cgcttcaaga gcggcacacg gcagaacgcc
     1861 atcatgcaag gcatggcttc cacgctggcg ccgcaggaca tgcacgacat cggcgcgtac
     1921 ttctcgcagc agaagcgcct gcccggcgtg gccgacgcca agctcgccag cgccggcggg
     1981 aagctgtacc gcgaaggcga ttccagccgc ggcatcccgg cgtgcatggc ctgccacggt
     2041 ccggatggcg cgggcaaccc gggctggcgc gtgccgaaca tcggcggcca gcacgccgat
     2101 tacatccagg cgcagctgaa agcctggcac gacggcacca cctggggcag cgacacgcac
     2161 gcgatgatca tgccgtcgat cgcccagcac ctgaccgagc aggacatctc ggcggtatcg
     2221 agctacgtcg agggcctgca tcccgcggcg gcgccgaaag ccaatccgaa gtccgacgcc
     2281 atcgattgaa cccgccgcgc ggcgcgtggt cgattcaggc gcaaagcggc acactcgcca
     2341 ccttccgcag ggaaaccgat catgatcaaa cgcaccgtag tgttcctgct gctcgccgcc
     2401 accgccggac tcgcgtccgc gcaggcgccg cagcaatgga ccgaaggcaa ggattacttc
     2461 gcgatccaga atccgcagcc caccgaccag cccgacaagg tggtggtgac ggaagtgttc
     2521 tcgttcgctt gcccggcgtg caaccagttc gagccttacc tcgacaaatt gcgcgcggaa
     2581 ctgccggcgg gcgcggtgct cgagttcctg cccgcgagct ggcatgcgaa cgaagattgg
     2641 ccggtgttcc agcgcgcgta cttcacggcg aaggcgctgg gcatcgacaa caagcaatcg
     2701 catgacgcgg tgttcaaggc gatctgggga ccgaacggtc cgctcgcgac ctacgatccg
     2761 acgacccagc gccccaaggc cgaagccaac ctgcccaagc tcgaagacgt cgcgaagttc
     2821 tacgcgcagt tcggcgccaa gcccgcggac ttcatcgcga ccgccgattc cttcacagtc
     2881 aatctgcaga tgaaacgcgc cgaccagcaa atcatggcct ggggcaccga cagcacgccg
     2941 acgctcgtga tcgacggcaa gtggcgcctg acgccggctt caaccaaggg tgcgcagcaa
     3001 gccgtcgacc tgacgctgta cctggtcaac aaggaactcg cggcaaagaa ggccaagtga
     3061 catgaacgca tcgcgcctgc ttcgcgcatc gttgctgatc tgcggcctgg tgttgaccgc
     3121 cgcgtgctcg gcgcacaacg acgccggcga cgtgtccgcg ccttccacgg cggcgacgcc
     3181 cgcgcccgcg gtcacggcga atttcaccga aggcaaggaa tacgtgaagc tggcgccgcc
     3241 gcaaggcacg cccgccccga ccggcgcggt ggaagtcgtc gaagtgtttt cctacggctg
     3301 cccgcattgc gccgagttc
//
LOCUS       DASZZC010000241         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1113952,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000241 DASZZC010000000
VERSION     DASZZC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113952"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VHE37_05185"
     CDS             complement(<1..246)
                     /locus_tag="VHE37_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVT34951.1"
                     /translation="MRPLALATFFVALGAAGAARADLYTCVDAQGHKTVSDKSCAPDQ
                     SATRVYSTPKTPGPAPAPGAVAPPDPLSPQSLDEIRAM"
     gene            complement(243..2018)
                     /locus_tag="VHE37_05190"
     CDS             complement(243..2018)
                     /locus_tag="VHE37_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVT34952.1"
                     /translation="MDFRSVLAESVLFSRLSPQALDVLAGKVGYHEISSGDVLFKHGE
                     PSQRLYTVASGRMRAMLPNGTIAGDIGRGESIGEIGLLAGEPHGATVYAVRDSELLSV
                     KREDLADLVQQFPAALLEATRVVIRRLRQNQASRKLESSRNNRAFALIPATRDVDVRP
                     VALALAEALRVYGSTRLLDPTAVDDALGSGAAQTQFSGNGGRNAELITWLNDLESVHR
                     YLVYTSNSPSDAWSRRCMRQADRVLVVADATSPAADSPTVDALKAYGTRAHVEMLMLR
                     RDTGPAGDVLGWRESIGARTHYFLRPGSKDDIASLARQITGRGIGLVLGGGGARGFAH
                     IGMLRALAELKIPIDSVGGTSMGAFFGALIACGYDPAQITEIARETYVKKNFLNDYLF
                     PTVALIRGRKFVRRLHEIFQDRQIEQLRTPFFCVSTNLTRGTAMVHDRGPLHLWLATS
                     MAVPGVAPPVGYRGELLADGAVVNSLPTDIMQSLERGPIIASDVSTEGDLRAPGVEGP
                     DPEALFHWGTVGKRPGLMSIIFRTATLTSESGVAARAARADLYVRMPVSGVGLFDWNT
                     LDEVSERGYRYALEKLSAVKSDLVK"
     gene            complement(2125..>2285)
                     /locus_tag="VHE37_05195"
     CDS             complement(2125..>2285)
                     /locus_tag="VHE37_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HVT34953.1"
                     /translation="PWQDTLEKLTGTRRMDASALIEYFTPLLDYLKQANQNATCGWDG
                     EAGAAPKT"
BASE COUNT          318 a          785 c          822 g          360 t
ORIGIN      
        1 catggcgcgg atctcgtcca ggctctgcgg tgagagcgga tcgggcggcg ccaccgcacc
       61 gggcgcaggc gcgggcccgg gggttttcgg cgtgctgtag acgcgcgtgg cgctctggtc
      121 tggcgcgcaa ctcttgtccg acaccgtctt gtggccctgc gcgtcgacgc aggtgtacag
      181 gtcggcccgc gccgcgccgg cggcgcccag tgctacgaaa aacgtagcaa gggccagcgg
      241 cctcacttga ccaggtccga cttcaccgcc gacagctttt ccagcgcgta gcggtagccg
      301 cgctcggata cttcgtcgag cgtgttccag tcgaacaggc cgacgccgga caccggcatg
      361 cgcacgtaca ggtcggcgcg cgccgcgcgc gcggccacgc cggactcgct ggtcagcgtg
      421 gcggtgcgga agatgatgct catcaggccc ggccgcttgc ccaccgtgcc ccagtggaac
      481 agcgcttcgg gatcggggcc ctcgacgccc ggtgcgcgca ggtcgccctc ggtggacacg
      541 tcggacgcga tgatcggtcc gcgctccagc gactgcatga tgtcggtagg caagctgttg
      601 accaccgcgc cgtcggccag cagttcgccg cggtagccca ccggcggcgc cacgcccggc
      661 accgccatgc tggtggccag ccacaggtgc agcgggccgc ggtcgtgcac catcgccgtg
      721 ccgcgcgtaa ggttggtcga cacgcagaag aacggcgtgc gcagctgctc gatctggcgg
      781 tcctggaaga tctcgtgcag gcgccggacg aacttgcgcc cgcggatcag cgccaccgtg
      841 gggaacaggt agtcgttgag gaagtttttc ttgacgtagg tttcgcgcgc gatctcggtg
      901 atctgcgccg ggtcgtagcc gcaggcgatc agcgcgccga agaacgcgcc catgctggtg
      961 ccgccgacgc tgtcgatcgg gatcttcagt tccgccagcg cgcgcagcat gccgatgtgg
     1021 gcgaagccgc gcgcgccgcc gccgccgagc accaggccga tgccgcggcc ggtgatctgc
     1081 cgcgccaggc tggcgatgtc gtccttgctg cccgggcgca ggaagtagtg cgtgcgcgcg
     1141 ccgatgctct cgcgccagcc gagcacgtcg ccggccgggc cggtgtcacg gcgcagcatc
     1201 agcatctcca cgtgcgcgcg cgtgccgtag gccttcaggg cgtcgaccgt cggcgaatcc
     1261 gccgccggcg aggtggcatc ggcgaccacc agcacgcggt cggcctggcg catgcagcgg
     1321 cgcgaccacg cgtcggacgg cgagttcgag gtgtagacca gatatcgatg cacgctctcc
     1381 aggtcattga gccaggtgat cagctcggcg ttgcgcccgc cgttgccgga aaactgggtc
     1441 tgcgccgcgc cgctgccgag cgcgtcgtcc accgcggtcg gatcgagcag gcgcgtgctg
     1501 ccgtagacgc gcagcgcttc cgccagcgcc agcgccaccg ggcgcacgtc gacgtcgcgc
     1561 gtggccggga tcagcgcgaa ggcgcggttg ttgcgcgacg actccagctt gcgcgaggcc
     1621 tggttctggc gcagccggcg gatgaccacg cgggtggctt cgagcagcgc ggcgggaaac
     1681 tgctgcacca ggtcggccag gtcctcgcgc ttgaccgaca gcagttcgga gtcgcgcacc
     1741 gcgtagaccg tggcgccgtg cggctcgccg gccagcaggc cgatttcgcc gatcgactcg
     1801 ccgcggccga tgtcgccggc gatggtgccg ttgggcagca tggcgcgcat gcggccgctg
     1861 gccacggtgt acaggcgctg gctcggctcg ccgtgcttga acagcacgtc gccgctggaa
     1921 atctcgtggt agcccacctt gccggcaagc acgtccagcg cctgcggcga caggcgcgag
     1981 aacagcacgc tttcggcgag tacggagcgg aaatccatgg cgtccctttg cgctgcgagt
     2041 ctagcccatt gcagcgccgg cttcggtttc caaaaaagcc agggccgcct gcgcggcccc
     2101 gagatctgat ttcctggcgc cgtttcaggt tttcggcgcg gcgccggctt cgccgtccca
     2161 gccgcaggtc gcgttctggt tggcctgctt gagatagtcc agcagcggcg tgaagtattc
     2221 gatcagcgcg gaggcgtcca tccggcgtgt gccggtgagt ttctccagcg tgtcctgcca
     2281 cggcc
//
LOCUS       DASZZC010000242         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_920821,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000242 DASZZC010000000
VERSION     DASZZC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920821"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..673
                     /locus_tag="VHE37_05200"
     CDS             248..673
                     /locus_tag="VHE37_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVT34954.1"
                     /translation="MQVVQWNPFREMEDMLARFGGSRALAKSGPLADWAPAVDIKETD
                     KEYSVKAELPGVGKDDVKVDVQNGVLTLSGERKFEKEDKDAKHHRIERAYGSFTRSFV
                     LPEDVLADKISAEHKDGVLCVHLPKTDIRKDKSTQIKIQ"
     gene            731..1285
                     /locus_tag="VHE37_05205"
     CDS             731..1285
                     /locus_tag="VHE37_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34955.1"
                     /translation="MVMPVPRQSLEISLTRISQLFNSLDPSPFYEKDLDTDAEEYLVS
                     WAQELHHDVPLALTIHLKETPPEVHPENWITQAIERHFRGRAEFTRLELRRLLRQGRI
                     ALVIGLGCMALFLLMAQWIAATHASVFSGLLRESLTVAGWVAMWRPMQIYLYDWWPLR
                     GRIRLYQRLSRMPVMVQLRTVASD"
     gene            1353..>2701
                     /locus_tag="VHE37_05210"
     CDS             1353..>2701
                     /locus_tag="VHE37_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT34956.1"
                     /translation="MSKQHEVKAAPDYLGALMDAAVDAIVLIGERGDIVRFNRSAEKL
                     FGYIAAEVIGRNVNCLMPEPYHTEHDGYLDRYHRTSEPRIIGIGREVVARRKDGSTFP
                     IDLSVGEFQSGDQHGFVGILRDISGRKEQERQLLRSSEELRLVFDNAPTAMLTSDPQG
                     RILNANRACCELLGYERGELLQLRHSDLMHAEDRPSGVSELQHLQQAGDSARRQMRFV
                     RKDGTVVHTLQYSAKTQDAEGRPLTIIEIVDRTEIYKATREAEELRMRLTHVARIGTL
                     GEMVSGIAHEVNQPLTAIANYASACRRMLNSGQAQPQELAGTLGKISDQAERAGQVIR
                     GLRALLRKRDAVREPLDCNQLVREVSALIDFELRQAGFRLLLQLAPQLPKTLGDGVQI
                     QQVMLNLIRNGLDAMQEHARGDFIQVSTAATGSWVQFVVSDSGPGLNPDVAERLFEP"
BASE COUNT          493 a          912 c          908 g          388 t
ORIGIN      
        1 ccggcctggg cgttggggtc gcgctcgcgc tcgatcccga tcagcaggcc tatgcccagg
       61 ctgctgacga tggccagcaa gccgtggaaa tccggtgcat tcgacatgcg cgcagtgtgc
      121 tgcccgcgcg cgcgcaccac cttgacctgg atcaagcgtg cagcttgatc caggtcaatg
      181 agccgcgtcg tccgccggcc catcctgcgc agcgccggat cggtccggca tcgaacagga
      241 gactcacatg caagtcgtac agtggaatcc cttcagggaa atggaagaca tgctggcgcg
      301 cttcggcggc agccgggcgc tggccaagag cggcccgctc gccgactggg cgcccgcggt
      361 cgacatcaag gagacggaca aggaatactc ggtcaaggcg gagctgcccg gcgtgggcaa
      421 ggacgacgtc aaggtcgacg tgcagaacgg cgtgctgacg ctgtccggcg agcgcaagtt
      481 cgaaaaggag gacaaggacg ccaagcacca tcgcatcgag cgtgcctacg gctcgttcac
      541 gcgcagcttc gtgctgccgg aagatgtgct ggccgacaag atcagcgcgg agcacaagga
      601 cggcgtgctg tgcgtgcatc tgcccaagac cgacatcagg aaggacaagt cgacccagat
      661 caagatccag tgaccgcccg cggtgccggc ggccgcctgc gccgccggca cccgcagccg
      721 ctgcttcgcc atggtcatgc ccgtaccgcg gcagtcgctg gagatctcgc tcacgcgcat
      781 cagccagctg ttcaactcgc tggacccgtc gccgttctac gagaaggacc tggacacgga
      841 cgccgaggaa tacctggtga gctgggcgca ggaactgcac cacgatgtgc cgctggcgtt
      901 gaccatccat ctcaaggaga cgccaccgga ggtgcatcct gaaaactgga tcacgcaggc
      961 catcgaacgg catttccgcg gccgcgccga attcacccgg ctggagttgc gccgcttgct
     1021 acgccagggt cgcattgccc tggtgatcgg gctcggctgc atggcgctgt tcctgctgat
     1081 ggcgcaatgg atcgcagcca cccacgccag cgtgttttcc ggcctgctgc gcgagagcct
     1141 gacggttgcc ggctgggtgg ccatgtggcg gccgatgcag atctacctgt atgactggtg
     1201 gccgttgcgc ggccgcatcc gcctgtacca gcgcctgagc cgcatgccgg tgatggtgca
     1261 gctgcgcacg gtggcgtccg actagccaca cgggcgcggg cgcgcgctac catggcgccg
     1321 cgcacgacag acagcaggac gggggcgcgg cggtgagcaa gcagcatgaa gtgaaggcag
     1381 cgcccgatta cctgggcgca ctgatggacg cggcggtcga cgccatcgtg ctgatcggcg
     1441 agcgcggcga catcgtgcgc ttcaaccgct ccgcggaaaa actgtttggc tacatcgcgg
     1501 ccgaagtcat cgggcgcaac gtcaactgcc tgatgccgga gccgtaccac accgagcatg
     1561 acggctacct cgaccgctac caccgcacca gcgagccgcg catcatcggc atcggccgcg
     1621 aggtggtggc gcggcgcaag gatggcagca cctttcccat cgacctgtcg gtcggtgaat
     1681 tccagagcgg cgaccagcat ggcttcgtcg gcatcctgcg cgacatcagc ggccgcaagg
     1741 aacaggagcg ccagctgctg cgcagcagcg aggaactgcg gctggtgttc gacaatgcgc
     1801 cgacggcgat gctcaccagc gacccacagg gccgcatcct caacgccaac cgcgcctgct
     1861 gcgagctgct gggctacgag cgcggggaac tgctgcagct gcgccattcc gacctgatgc
     1921 acgccgagga ccgcccgtcc ggcgtcagcg aactgcagca cctgcagcag gccggcgact
     1981 cggcgcgccg gcagatgcgc ttcgtgcgca aggacggcac ggtggtgcac acgctgcagt
     2041 attcggccaa gacccaggac gccgagggcc ggccgctcac catcatcgag atcgtcgacc
     2101 gcaccgagat ctacaaggcc acgcgcgagg ccgaggaact gcgcatgcgc ctgacccacg
     2161 tggcgcgcat cggcacgctc ggcgaaatgg tcagcggcat tgcccacgag gtgaaccagc
     2221 cgctcaccgc aatcgccaac tacgccagcg cctgccggcg catgctcaac tcaggccagg
     2281 cccagccgca ggaactggcc ggtaccctgg gcaagatcag cgaccaggcc gagcgagccg
     2341 ggcaggtcat ccgcggtttg cgcgcattgc tgcgcaagcg cgacgcggtg cgcgagccgc
     2401 tcgactgcaa ccagctggtg cgcgaggtgt ccgcgctgat cgacttcgag ctgcgccagg
     2461 ccggcttccg cctgctgctg cagctggcgc cgcagctgcc caagacgctg ggcgacggcg
     2521 tgcagatcca gcaggtgatg ctcaacctga tccgcaacgg ccttgacgcg atgcaggaac
     2581 acgcgcgcgg cgacttcatc caggtgagca ccgccgccac gggaagctgg gtgcagttcg
     2641 tggtcagcga cagcggcccg ggcctcaacc ccgacgtggc cgagcggctg ttcgaaccgt
     2701 t
//
LOCUS       DASZZC010000243         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_81724,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000243 DASZZC010000000
VERSION     DASZZC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81724"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2012
                     /locus_tag="VHE37_05215"
     CDS             <1..2012
                     /locus_tag="VHE37_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HVT34957.1"
                     /translation="HHFRKLGAAVAAPPHAGTGSGGSLTPADLRKGYNADSIQNGGQT
                     VALYELSTATYADAATYASHFGLNNPTLQLINVDGGTTDNSGAGEVMLDIEVVMALAN
                     PGKIIVYTAPNGSGILDLYQRIADDHLSDFVSTSWGINEASEGQANANAEATIFQKMV
                     GEGMTTFAASGDAGAYDGGGLALKVDDPASQPNVTGVGGTTLQLDASQNYVKESAWGD
                     STDKNGTLKGSGSGGGISTLWSIPSYQNGIVSSAPSGQFSTTMRNVPDVSLAGSFDDA
                     DSCYYIYYTDPSSGAGWYLVSGTSAAAPVWAAYWSLVGKGLADVQLTPAPPGFANTTL
                     YALAKNSGTYVNDFHDVNDGTTNLHFKAVTGYDTATGWGSFNGANLYADAISSITGGN
                     VPAAPTNLQATAGQNKVTLNWTASATSGVTYSVYRGTASGGEAGSAVASGISGTSTVI
                     SGLTAGTAYFFEVRAVGSSYRSAVSNEASATPTAQAAAPPAPLTLTAKPGADGVIKLS
                     WTVSGEPANVTYRVYQGTAAGGETGAPAKSSIKATSTTISGLTDGTPYYFTVKAYDGS
                     RLSTASPEASATPFGPPTGMSATAGKGKVVLHWNASPGAEKYRVYQGNTAGGESSTAV
                     KSGIALTSTTISGLTPGLPYFFTVKATQGSTVSAASAEVRATPTP"
     gene            complement(2026..2304)
                     /locus_tag="VHE37_05220"
     CDS             complement(2026..2304)
                     /locus_tag="VHE37_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34958.1"
                     /translation="MNPAFSSRCFFAVEAENDPTVATRLLHLLTVRGQTPEWFSLRKH
                     RPEALRISVEVAGVDDAAARQLAQQMQRIPAVIGVSRSWLARRDALTP"
     gene            2515..>2993
                     /locus_tag="VHE37_05225"
     CDS             2515..>2993
                     /locus_tag="VHE37_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34959.1"
                     /translation="MSPMYNLLVTLVPRAWERTAFVLERERVLEYSDAPLQKKFRALS
                     ARAAGQLLQLPCLFAYEQQVGADARLGHITRIQRRRNDVRMEFRIDRASPPVPRELLD
                     KSRSDFDIAAGELSRTHWAVKDVALLEMLEARGLLAARERRRLQRALQADADAVQVRR
                     "
BASE COUNT          536 a         1050 c         1003 g          404 t
ORIGIN      
        1 atcatcattt ccgcaagctc ggcgccgcgg tggccgcgcc gccgcatgcc ggcaccggct
       61 cgggcggctc gctcacaccg gcggacctgc gcaagggcta caacgccgac agcatccaga
      121 acggcgggca gacggtggcg ctgtacgaac tttccaccgc gacctatgcc gatgccgcca
      181 cctacgcctc gcacttcggc ctgaacaatc ccacgctgca gctgatcaat gtcgatggcg
      241 gcaccaccga caacagcggc gccggcgagg tgatgctcga catcgaggtg gtgatggcgc
      301 tggccaatcc cggcaagatc atcgtctaca ccgcgcccaa cggcagcggc atcctcgatc
      361 tgtaccagag gattgccgac gatcacctgt cggatttcgt cagtacctcg tggggcatca
      421 acgaggcgag tgaaggccag gccaacgcca acgccgaggc gacgattttc cagaagatgg
      481 tgggcgaggg catgaccacc ttcgccgctt ccggcgatgc cggcgcgtac gacggcggcg
      541 gccttgcgct gaaggtggat gatccggcgt cgcagcccaa cgtcaccggc gtcggcggta
      601 ccacgctgca gctcgatgcc tcgcagaact atgtgaagga gtcggcctgg ggcgactcga
      661 ccgacaagaa cggcacgctc aagggttccg gcagcggcgg cggcatcagc accctgtgga
      721 gcattccctc gtaccagaac ggcatcgtgt catcggcgcc gtccgggcag ttctccacca
      781 ccatgcgcaa cgtgcccgac gtatcgctgg ccggcagctt cgacgacgcg gactcctgct
      841 actacatcta ctacaccgat ccctccagcg gcgcgggctg gtacctggtg agcggcacca
      901 gcgctgccgc gccggtgtgg gccgcgtact ggagcctggt cggcaagggc ctggccgacg
      961 tgcagctgac gccggcgccg cccggtttcg ccaacaccac gctgtacgcg ctggcgaaaa
     1021 actccggcac ttacgtcaac gattttcacg acgtgaacga cggcaccacc aacctgcact
     1081 tcaaggcggt gaccggctac gacaccgcca ccggctgggg ctcgttcaac ggcgccaacc
     1141 tctatgccga cgccatcagc tcgatcaccg gcggcaacgt gccggccgcg ccgaccaatc
     1201 tccaggccac tgccggacag aacaaggtga ccttgaactg gaccgcttcg gccacgtccg
     1261 gcgtgaccta ctcggtgtat cgcggtacgg ccagcggcgg cgaagcgggc agcgccgtcg
     1321 ccagcggcat cagcggcacc agcaccgtga tcagcgggct gaccgcgggc accgcctatt
     1381 tcttcgaggt gcgcgcggtg ggctcgagct accgcagcgc ggtttccaac gaagccagcg
     1441 ccacgccgac ggcgcaggcc gccgcgccgc cggcaccgct gacgctcacg gcgaaacccg
     1501 gcgccgacgg cgtgatcaag ctgagctgga cggtcagcgg cgagccggcc aacgtgacct
     1561 accgcgtgta ccagggcacc gcggcgggcg gcgagaccgg cgcgccagcc aagagctcga
     1621 tcaaggccac cagcaccacg atcagcgggc tcaccgacgg caccccgtat tacttcacgg
     1681 tcaaggccta cgacggcagc aggctcagca ccgcgtcgcc ggaagccagt gccacgccgt
     1741 tcggcccgcc cacgggcatg agtgcgaccg cgggcaaggg caaggtggtg ctgcactgga
     1801 acgcgagtcc gggcgccgag aaataccgcg tctaccaggg caacacggcc ggcggcgaaa
     1861 gcagcacggc ggtgaagagc ggcatcgcgc tgaccagcac caccatcagc gggctgacgc
     1921 ccggcctccc ctatttcttc acggtgaagg ccacgcaggg cagcacggtc agcgccgcat
     1981 cggccgaggt gagagccacg cccacgccgt gacgcgcacg cgccgctagg gggtgagcgc
     2041 gtcgcgccgc gccagccacg agcgcgacac gccgatcacc gcggggatgc gctgcatctg
     2101 ctgcgccagc tggcgcgcgg cggcgtcatc cacgccggcc acttccaccg agatgcgcag
     2161 cgcctcgggc cggtgcttgc gcaggctgaa ccattcggga gtctggccgc gcaccgtgag
     2221 caggtgcagc aggcgggtgg cgaccgtcgg gtcgttttca gcttcgacgg cgaagaaaca
     2281 gcgggacgaa aaagcagggt tcatggcgga tctctgaagt cggcaaggca acgcacggcg
     2341 cggcgacgca gctcgtaatt cgagccgcgc gaggcattcg ccgggtgcgt actgacgtca
     2401 tcgatccatc catcgttgcg gccaccacgg ccgcgagggc gcgcagtcta accgaagccc
     2461 cgcgccgctg cgcaagccgt aaactccgtc gccgccgaag ggagcgggac atggatgtcg
     2521 cccatgtata acctgctggt cacgctggtg ccgcgcgcat gggagcgcac cgcgttcgtg
     2581 ctcgaacgcg agcgcgtgct ggaatacagc gacgcgccgc tgcagaaaaa attccgcgcg
     2641 ctgtccgcgc gcgccgccgg acagctgctg cagctgccct gcttgtttgc ctacgaacag
     2701 caggtcggtg ccgatgcgcg cctgggccac atcacccgca tccagcggcg caggaacgat
     2761 gtgcgcatgg aattccgcat cgaccgcgcc agcccgccgg tgccgcgcga actgctcgac
     2821 aagtcacgct cggatttcga catcgccgcc ggcgaactct cgcgcacgca ctgggcggtg
     2881 aaggacgtgg cgctgctgga gatgctcgaa gcgcgcgggc tgctggcggc gcgcgaacgg
     2941 cggcgcctgc agcgcgcgct gcaggccgat gcggatgcgg tgcaggtgcg gcg
//
LOCUS       DASZZC010000244         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1044387,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000244 DASZZC010000000
VERSION     DASZZC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044387"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VHE37_05230"
     CDS             <1..1017
                     /locus_tag="VHE37_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVT34960.1"
                     /translation="GSGVAAAASLATLTVGTETDGSIVCPASVNGVVGLKPTVGLVSR
                     SGIIPIAASQDTAGPITRTVAEAALLLGVLAGADVDDPATQEREPGEPPDYLAALSLD
                     GLKGARIGVARNLGGFHEQVDAVFEQALSALREAGATLVDPADIKLAASAEAREATVL
                     SYEFKDGINHYLAHRDAGPRDLADLIAYNKEHAQTELAWFGQELFLQAQQRGPLTDAA
                     YRHAQHHARHAARKAIDDALAQHQVDVIVAPTMGAAWTTDLINGDHIIGGSASAAAAI
                     AGYPHLTVPMGYVHELPVGLSFIGTAWSEPLLLKLGYAYEQRAQVRRPPQYAAQPALA
                     AVRQ"
     gene            1046..1912
                     /gene="ttcA"
                     /locus_tag="VHE37_05235"
     CDS             1046..1912
                     /gene="ttcA"
                     /locus_tag="VHE37_05235"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HVT34961.1"
                     /translation="MSAVVTHPIADLRPARRDDARESNKLVKRLRRQVGQAIMDYRMI
                     GEGDRVMVCLSGGKDSYTMLDLLLQLQQKAPVKFSLTAVNLDQKQPGFPEHVLPQYLK
                     SLGVDYEILEQDTYSVVKRVIPEGKTMCSLCSRLRRGALYAHAKARGYTRIALGHHKD
                     DIVATFFLNLFHGGKLAAMPPKLLSDDGAHVVIRPLAYCRERDIVRYAEQKQFPIIPC
                     NLCGSQEQLQRRQVRRMMDEWEQAHGARIEQVFAALTNVAPSQLADARLFDFAGLGGN
                     AAAVDWLMPDAE"
     gene            1921..2604
                     /locus_tag="VHE37_05240"
     CDS             1921..2604
                     /locus_tag="VHE37_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HVT34962.1"
                     /translation="MADHPYRDPKKIYFFFDYISHNAYLAWHRLPELAQRHELIIEPV
                     PVLFAALLKAYNQVGPAELPVKSGWMLKNVLRKARKLQLPMAPPASHPFNPLAALRLS
                     CCELPRAARADLITRLYAGTWAQSRAMHEPAVLQEIVREAGFDADALLAEADSEPVKR
                     QLRDNGEQALAAGVFGVPTMIVRGELFWGYDDWQQLEDFLAGRDPLGHDGSALAGWDA
                     VRPSAQRRK"
     gene            2601..>3102
                     /locus_tag="VHE37_05245"
     CDS             2601..>3102
                     /locus_tag="VHE37_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HVT34963.1"
                     /translation="MSLRKILAAMVLLGAGAAQAADAPFLWEVSGARNKHYLMGSVHM
                     LPESAYPLPPALDTAYAAADALVLETDIAALTSPQLQAQMLSAGASPSGLKNEIGAPL
                     YQRVQDYAHAHGIPGQLCDAFKAWFCSLTLEVLSFQQTGSRPDLGLDQHYYMQALAAR
                     KAVLWLE"
BASE COUNT          497 a         1079 c         1077 g          449 t
ORIGIN      
        1 ggttcgggtg tggcggcggc ggccagcctg gccacgctta ccgtcggcac cgagaccgac
       61 ggttcgatcg tgtgcccggc cagcgtcaac ggcgtggtcg ggctcaagcc cacagtcggg
      121 ctggtcagcc gcagcggcat catcccgatt gccgccagcc aggacaccgc ggggccgatc
      181 acgcgcacgg tggcggaggc ggcgctgctg ctcggcgtgc tggcgggcgc ggacgtcgat
      241 gacccggcca cgcaggagcg cgagcccggc gaaccgccgg attacctggc cgcgctgtcc
      301 ctcgacggac tcaagggcgc gcgcatcggc gtggcgcgca atctcggcgg attccacgaa
      361 caggtcgacg cggtattcga gcaggcgctg tccgcgctgc gcgaagccgg tgccacgctg
      421 gtcgatcccg ccgacatcaa gctggcggcc agcgccgagg cgcgcgaggc caccgtgctg
      481 tcctacgagt tcaaggacgg catcaaccac tacctcgcgc accgcgacgc gggcccgcgc
      541 gacctggcgg acctgatcgc ctacaacaag gaacacgcgc agaccgaact ggcctggttc
      601 ggccaggagc tgttcctgca ggcgcagcag cgcggcccgt tgaccgacgc cgcctaccgc
      661 catgcgcagc atcacgcgcg ccatgccgca cgcaaggcga tcgacgacgc gctggcgcag
      721 caccaggttg acgtgatcgt ggcgccgacc atgggcgcgg cctggaccac cgacctgatc
      781 aacggcgacc acatcatcgg cggcagcgcc tccgccgcgg ccgccatcgc cggctatccg
      841 cacctgaccg tgcccatggg ttacgtgcac gagctgccgg tgggtttgtc gttcatcggc
      901 accgcctgga gcgagccgct gctgctcaag ctgggctacg cctatgagca gcgggcgcag
      961 gtgcggcggc cgccgcagta cgcagcccag cccgcgctcg ccgccgtgag acaatagcgg
     1021 ccctattttt ccgcggtgct gtccgatgtc tgccgtcgtc acccatccca tcgccgacct
     1081 gcgccccgcg cgccgcgacg atgcgcgcga gtccaacaag ctggtcaagc ggctgcgccg
     1141 ccaggtgggc caggccatca tggattaccg catgatcggc gaaggcgacc gcgtcatggt
     1201 ctgcctgtcc ggcggcaagg acagctacac catgctcgac ctgctgctgc agctgcagca
     1261 gaaggcgccg gtgaagttct cgctcaccgc ggtcaacctc gaccagaagc agcccggctt
     1321 tcccgagcac gtgctgccgc agtacctgaa gtcgctgggc gtcgattacg agatcctcga
     1381 gcaggacacc tactcggtgg tcaagcgggt gatcccggaa ggcaagacca tgtgctcgct
     1441 gtgctcgcgc ctgcgccgcg gcgcgctgta cgcgcacgcc aaggcgcgcg gctacacgcg
     1501 catcgcgctg ggccatcaca aggacgacat cgtcgccacg tttttcctca acctgttcca
     1561 cggcggcaag ctggcggcca tgccgcccaa gctgctgtcg gatgacggcg cgcatgtggt
     1621 gatccgcccg ctggcgtact gccgcgagcg cgacatcgtg cgctacgcgg agcagaagca
     1681 gttccccatc atcccgtgca acctgtgcgg ctcgcaggag cagctgcagc gcaggcaggt
     1741 gcggcgcatg atggacgagt gggagcaggc gcacggcgcg cgcatcgagc aggtgttcgc
     1801 cgcgctgacc aacgtggcgc cgtcgcagct ggccgacgcg cggctgttcg atttcgccgg
     1861 cctcggcggg aatgcagctg cggttgactg gctgatgccg gacgcggagt gagcgcgcgc
     1921 atggccgacc atccgtaccg cgatccgaaa aagatctact tcttcttcga ctacatcagc
     1981 cacaacgcct atctcgcctg gcaccgcctg ccggaactgg cacagcggca cgagctgatc
     2041 atcgagccgg tgccggtgct gttcgccgcg ctgctcaagg cctacaacca ggtgggcccg
     2101 gccgaactgc cggtgaagag tggctggatg ctgaagaacg tgctgcgcaa ggcgcgcaag
     2161 ctgcagctgc cgatggcgcc gccggcatcg cacccgttca acccgctggc cgcgctgcgc
     2221 ctgtcgtgct gcgagctgcc gcgcgcggcg cgcgccgacc tgatcacgcg cctgtacgcg
     2281 ggcacctggg cgcaaagccg cgccatgcat gagccggcgg tgctgcagga gatcgtgcgc
     2341 gaagccggct tcgatgccga cgcgctgctg gccgaggccg acagcgaacc ggtgaagcgg
     2401 caactgcgcg acaacggcga gcaggcgctg gccgccggcg tgttcggcgt gccgaccatg
     2461 atcgtgcgcg gcgaactgtt ctggggctac gacgactggc agcagctgga agactttctc
     2521 gccgggcgcg atccgctggg ccacgacggc agcgcgctgg ccggctggga tgcggtgcgg
     2581 ccttcggcgc agaggcgcaa atgagtttgc ggaaaattct ggcggcgatg gtcctgctcg
     2641 gcgccggcgc agcgcaggcg gccgacgcgc cgttcctgtg ggaagtgagc ggcgcgcgca
     2701 acaagcacta cctgatgggc tcggtgcaca tgctgccgga gagcgcgtac ccgctgccgc
     2761 cggcgctcga cacggcctac gccgccgccg atgcgctggt gctggaaacc gacatcgccg
     2821 cgctcaccag cccgcaactg caggcgcaga tgctgtcggc gggcgcctcg ccgtcggggc
     2881 tgaagaacga gatcggcgca ccgctgtacc agcgcgtgca ggactacgcg cacgcgcatg
     2941 gaattcccgg ccagctgtgc gatgcgttca aggcctggtt ctgctcactg acgctggaag
     3001 tgctgagctt ccagcagacc ggcagccggc ctgacctggg cctcgaccag cactactaca
     3061 tgcaggcgct ggcggcgcgc aaggcggtgc tgtggctgga ag
//
LOCUS       DASZZC010000245         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_853614,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000245 DASZZC010000000
VERSION     DASZZC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853614"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VHE37_05250"
     CDS             complement(<1..550)
                     /locus_tag="VHE37_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34964.1"
                     /translation="MRPALPPRVRAAVTGALSFRTQLLLFAAALMLPVGAVTAWLAYT
                     DFRNDIAAGRESTRGLARLAAAQYDFFLRVTDERLRRLAQRPRVAAADPARCDPVGTE
                     LIALQPAYLNLWTLLPDGRIACSALPVPRDSGPTLAQQPWLAEALRAPGMHVSEPFAS
                     PVSGERVSMLSYPLRGGARVALV"
     gene            737..2146
                     /locus_tag="VHE37_05255"
     CDS             737..2146
                     /locus_tag="VHE37_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HVT34965.1"
                     /translation="MDAASFTPAGCRAPRWLPLLALLVTVPAHALSYRMPPDAALVDG
                     APTVVLGHIESAAADPQRPNSATLYQLDVDRLLKGLAAAAILRVRVPGAYDATQPGSL
                     VVPGAPVFMPGERLLLMLQPRGDGSYAISQLALGAFHVRSIDGIDVLLRQPDQAAPQQ
                     WRRLDAFGSWISSRAGGLLAAADYWVQLTGNVPALKYALEGSPPVRWFDFDQGRSVTF
                     YSSEQGQIGLLDGGVAEFRRAIAAWNADTGSNINYVYGGLSSASGGMDHADGVNEILF
                     NDPNGELSGVFDCTNGGVIAYGGYWTSGNKSTHRDGTFQMISEADIVVQNGAGCILSA
                     LSNSNADEVFAHELGHTLGLAHPCGESGVPACTPGSAVDDAIMRPYVHADSRGASLRS
                     DDQQAIAYDYSSGSSPPPPAAVGRGDPEAGIPNSSDSSSSGGGGALDATLLLALALAL
                     WRRAIRRFWWSPQRYFACY"
     gene            complement(2225..>2753)
                     /locus_tag="VHE37_05260"
     CDS             complement(2225..>2753)
                     /locus_tag="VHE37_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT34966.1"
                     /translation="VRSIVELHGGSVRAVSAGPGRGSEFIVRLPALAAAPAQAEPPEA
                     AAEGERKGRRILVVDDNKDAADSLAILLREAGHTVETAYSGVSALKLAVSFHPEVILL
                     DIGLPGMDGYEVAGRVRDMPEIGEPLVLALTGYAQPADRERARVSGFDHYLIKPADFK
                     ELQALLARPHGQRVH"
BASE COUNT          419 a          989 c          943 g          402 t
ORIGIN      
        1 ctaccagcgc cacgcgcgcg ccgccgcgca gcgggtagct gagcatgctc acgcgctcgc
       61 ccgacaccgg gctggcgaac ggctcgctca catgcatgcc cggcgcgcgc agcgcctcgg
      121 ccagccacgg ctgctgcgcc agcgtgggac cactgtcgcg cggcaccggc agcgccgagc
      181 aggcaatgcg gccgtcgggc agcagcgtcc acaggttgag gtacgccggc tgcagggcga
      241 tcagctcggt gccgaccgga tcgcagcgcg cggggtcggc ggcggccacg cgcgggcgtt
      301 gcgccagccg gcgcaggcgt tcgtcggtca cgcgcaggaa gaagtcgtat tgcgccgccg
      361 ccagccgggc cagcccgcgc gtgctctcgc gcccggcggc gatgtcgttg cggaaatcgg
      421 tgtaggccag ccaggcggtg accgcgccca ccggcagcat cagcgcggcg gcgaacaaca
      481 gcagctgggt gcggaacgac agcgcgccgg taaccgctgc gcgcacgcgc ggcggcaagg
      541 ccgggcgcat cgccattcct ccggtccggt cgcccatgtc cccatccatt gagacacacc
      601 tgccagcaac gtctgcggct tcgctgccgc ctgctggagt caagcataca cccgcatccc
      661 gtcaggacaa cctgacagaa cctacagggt gcgcaggcct agcgtggctg cacggatcaa
      721 ccacgcgggg aacaacatgg acgctgcctc tttcacgcct gccggctgcc gcgcgccgcg
      781 ctggctgccg ctgctggccc tgctggtgac cgtgccggcg catgcgctca gctaccggat
      841 gccgcccgat gccgcgctgg tcgacggcgc gcccaccgtg gtgctcggcc acatcgaatc
      901 ggcggccgcc gatccacagc gcccgaacag cgccaccctt taccagctgg acgtggaccg
      961 gctgctcaag gggctggcgg cggccgccat cctgcgcgtg cgcgtgcctg gcgcctacga
     1021 cgcgacgcag cccggctcgc tggtggtgcc gggcgcgccg gtgttcatgc cgggcgagcg
     1081 cctgctgctg atgctgcagc cgcgcggcga cggcagctat gccatcagcc agctggcgct
     1141 cggcgcgttc cacgtgcgca gcatcgacgg catcgacgtg ctgctgcgcc agcccgacca
     1201 ggccgcgccg cagcagtggc gccggctcga tgccttcggc agctggattt catcgcgcgc
     1261 cggcgggctg ctcgccgccg ccgattactg ggtgcagctc accggcaatg tgccggcgct
     1321 caagtacgcg ctggaaggct caccgccggt gcgctggttc gacttcgacc agggtcgcag
     1381 cgtgacgttt tactccagcg agcaggggca gatcggcctg ctcgacggcg gcgtcgccga
     1441 attcaggcgc gccatcgccg cctggaacgc cgacaccggc agcaacatca actacgtgta
     1501 cggcggactc agttcggcca gcggcgggat ggaccatgcc gacggcgtca acgagatcct
     1561 gttcaacgat cccaacggcg agctgtccgg cgtgttcgac tgcaccaacg gcggcgtcat
     1621 cgcctacggc ggctactgga cctcgggcaa caagagtacg catcgcgacg gcacgttcca
     1681 gatgatcagc gaggccgaca tcgtggtgca gaacggcgcc ggctgcatcc tgtcggcgct
     1741 gagcaacagc aacgccgacg aagtgttcgc gcatgaactc ggccacacgc tgggcctggc
     1801 gcatccctgc ggcgagagcg gcgtgccggc gtgcacgccc gggtccgcgg tggatgacgc
     1861 catcatgcgc ccctacgtgc atgccgacag ccgcggtgct tcgctgcgca gcgacgacca
     1921 gcaggccatt gcgtacgact attccagcgg ctccagcccg ccgccgccgg ccgccgtcgg
     1981 caggggcgat ccggaagccg gcatacccaa cagcagcgac agcagcagca gcggcggcgg
     2041 cggcgcgctc gatgccacgc tgctgctggc gctcgcgctg gcgctgtggc ggcgcgcgat
     2101 caggcgtttc tggtggtcac cgcaaaggta ttttgcgtgt tattaattat tatcttaaat
     2161 taaaataata taatttttca taacatgaga aaagccggtg cggtgcatgt cctggagccg
     2221 cgattcagtg cacgcgctgg ccgtgtggcc gcgccagcag cgcctgcagt tccttgaagt
     2281 cggccggctt gatcaggtag tggtcaaaac ccgagacgcg cgcgcgctcg cggtcggccg
     2341 gctgcgcgta accggtcagc gccagcacca gcggctcgcc gatctcgggc atgtcgcgca
     2401 cgcgtccggc gacttcgtag ccgtccatgc cgggcaggcc gatgtcgagc aggatcactt
     2461 ccgggtgaaa cgacacggcg agcttgagcg cggacacacc gctgtacgcg gtctccaccg
     2521 tgtggcccgc ttcgcgcagc aggatggcca ggctgtcggc ggcgtccttg ttgtcgtcca
     2581 ccaccaggat gcggcgaccc ttgcgctcgc cttccgctgc cgcctcgggc ggctcagcct
     2641 gcgccggcgc ggccgccagc gccggcaggc gcacgatgaa ctcgctgccg cggcccggcc
     2701 ccgcgctcac cgcgcgcacg ctgccgccgt gcagttccac gatgctgcgc acc
//
LOCUS       DASZZC010000246         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1114410,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000246 DASZZC010000000
VERSION     DASZZC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114410"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VHE37_05265"
     CDS             complement(<1..574)
                     /locus_tag="VHE37_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34967.1"
                     /translation="MRYLRISRFIRWLLSPRNEAREPGFDAHRYQRQRRLGFPMLRFL
                     PELEREYRESFFNVNVMRLRLAHVIGLLGCFGFIAMDRWLGYQLQTPASNAVLLGICC
                     PALALPMAATFVASLHRYLSSIIFAGALGLGLGLVDVVYLGRVANEWFPYESIFLVTI
                     YVYYLSGLLWIESMICGALIWNAYLFMDAKG"
     gene            810..1949
                     /locus_tag="VHE37_05270"
     CDS             810..1949
                     /locus_tag="VHE37_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HVT34968.1"
                     /translation="MHAKVSPAPETLPARKPLRFVTAASLFDGHDAAINIMRRLIQQL
                     GGEVIHLGHNRSVEEVVTAALQEDAHGIAISSYQGGHVEYFKYMVDMLKERGGANIQV
                     FGGGGGVIVPEEIKELHAYGVSRIYSPHDGQKLGLQGMIQDMLDRAAHELDGKPDELL
                     LKVRGGDRRALARLITLIEAGKVDASKLFSPSAPGGEGRAEGSRAPARVIPVLGITGT
                     GGAGKSSVSDELIRRFRLDQNDALKIAVIAVDPSRRKSGGALLGDRIRMNAINHPNIF
                     FRSLATRESGMEISPALPGAIAACKAAGFDLIFAETAGIGQGDAAVVEHADVSLYVMT
                     PEFGAASQLEKIDMLDFADVVAINKFERRGALQRRRRYRTVFWRA"
     gene            1957..>2314
                     /locus_tag="VHE37_05275"
     CDS             1957..>2314
                     /locus_tag="VHE37_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34969.1"
                     /translation="MAKGLDLSRHSRAGGNPVGGRDESKSKLDSGLRRNDEGFLARPA
                     TKHSTSARQIVPGARARYLAEIADTNRGYRKRAEHQAQLARERQHLRETIRMMEHSAH
                     PVEDLKAEMHKRLDHLT"
BASE COUNT          446 a          790 c          731 g          347 t
ORIGIN      
        1 agcccttggc gtccatgaac aggtaggcgt tccagatcag cgcgccgcag atcatcgact
       61 cgatccacag caggccggac aggtagtaca cgtagatggt gaccaggaag atcgattcgt
      121 acgggaacca ctcgttggcc acgcgcccga ggtacaccac gtcgaccagg cccagcccga
      181 ggccgagcgc gccggcaaag atgatcgacg acaggtagcg gtgcagcgac gctacgaagg
      241 tggcggccat cggcagcgcc agcgccgggc agcagatgcc cagcagcacg gcgttggagg
      301 ccggcgtctg cagctggtag ccgagccagc ggtccatggc gatgaagccg aaacagccga
      361 gcaggccgat cacgtgcgcc aggcgcaggc gcatgacgtt gacgttgaag aacgactcgc
      421 ggtactcgcg ctccagctcg ggcaggaagc gcagcatggg aaaacccagc cgccgctgcc
      481 gctggtagcg gtgcgcgtca aagcctggct cgcgcgcctc gttgcgcggc gacagcagcc
      541 atcggatgaa acgagaaatg cgcaggtatc tcaccgcgtc cgcctccccc tcgggacgcc
      601 ccctccggtg atgccggatt atgggcgttt cgcgcgggga ttcagtttga tcaccgtcac
      661 attgcatgca atttctaggc tgattttttc gctgcgctgc ggcccgctgg cctccctgat
      721 cggacgaggt cggaccagtg caagttcggg atactgcgcg ccctataatt ccgactccag
      781 aaacgctcga aaaacgtcac tctctgacaa tgcacgccaa agtctcgccc gctccggaaa
      841 cgcttcccgc ccgcaagccg ctgcgcttcg tcaccgccgc ttcgctgttc gacggccacg
      901 atgccgccat caacatcatg cgccggctga tccagcagct cggcggcgaa gtgatccacc
      961 tgggccacaa ccgcagcgtg gaagaagtgg tcaccgccgc gctgcaggaa gacgcgcacg
     1021 gcatcgccat cagcagctac cagggcggcc acgtcgagta cttcaagtac atggtcgaca
     1081 tgctcaagga acgcggcggc gccaacatcc aggtgttcgg cggcggcggc ggcgtgatcg
     1141 tgccggaaga gatcaaggaa ctgcacgcgt atggcgtgag ccgcatctac tcgccgcacg
     1201 acggccagaa gctgggcctg cagggcatga tccaggacat gctcgaccgc gccgcgcacg
     1261 agctcgacgg caaacccgac gagctgctgc tcaaggtgcg cggcggcgac cggcgcgcgc
     1321 tggcacgcct catcacgctg atcgaggccg gcaaggtcga cgcaagcaaa ctcttttctc
     1381 cctctgcccc cgggggagag ggtcgggctg agggctcgcg cgcgccagca cgcgtgatcc
     1441 cggtgctcgg catcaccggc accggcggcg cgggcaagtc gagcgtgagc gatgaactca
     1501 ttcgccgctt ccgcctggac cagaacgacg cgctcaagat cgcggtgatc gcggttgatc
     1561 cttcgcgccg caagtccggc ggcgcattgc tgggcgaccg catccgcatg aatgcgatca
     1621 accaccccaa catcttcttc cgctcgctgg ccacgcgcga aagcggcatg gagatctccc
     1681 ccgccctgcc cggcgccatc gccgcgtgca aggccgcggg ctttgacctg atcttcgcgg
     1741 agaccgccgg catcggccag ggcgacgccg ccgtggtcga gcacgcagac gtttcgctct
     1801 acgtgatgac gccggagttc ggcgcggcca gccagctgga gaagatcgac atgctcgact
     1861 tcgccgacgt ggtagcgatc aacaagttcg agcggcgcgg cgcgcttcaa cgacgacggc
     1921 gttaccgcac tgtattttgg cgtgcgtgat ctgctcgtgg ccaagggcct cgatctttcc
     1981 cgtcattccc gcgcaggcgg gaatccagtt ggcggtagag atgagagcaa atcaaaactg
     2041 gattccggcc ttcgccggaa tgacgaagga tttttggcgc gtccggccac caagcactct
     2101 acctccgccc gccagatcgt ccccggcgcc cgcgcccgct acctcgcgga aatcgccgac
     2161 accaaccgcg gctaccgcaa gcgcgcggaa caccaggcgc agctcgcgcg cgagcgccag
     2221 cacctgcgcg agaccatccg catgatggaa cacagcgccc acccggtcga agacctcaag
     2281 gccgagatgc acaagcgcct cgaccatctc accg
//
LOCUS       DASZZC010000247         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_596953,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000247 DASZZC010000000
VERSION     DASZZC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596953"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..837)
                     /locus_tag="VHE37_05280"
     CDS             complement(7..837)
                     /locus_tag="VHE37_05280"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVT34970.1"
                     /translation="MNLLQNYWVHGEKLADLAVTGLFNLIAALAIFFSGKWLAQMLLK
                     LLRVLMRRGRMDETLAGFLGHVLYGLALVVVVFTALAQLGVNTASAAAVLGGAALAIG
                     LSLQAQLASFAAGVVIIIFRPFNLGDYVEIGGQRGFVEEIKIVTTQLRTLDNKLVIVP
                     NSSITSNIITNFTARPTRRIDITVPLPHEADLMQAKQLLAEVLNGDARVLKQPAPAVS
                     VKSISRDGVELLVSPWVHTPERGDVEATLLEAIKLKLDQARVPLAAAPARIELKSALS
                     "
     gene            903..1760
                     /locus_tag="VHE37_05285"
     CDS             903..1760
                     /locus_tag="VHE37_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT34971.1"
                     /translation="MNDPAQIRLQANGVDFCALELGRGPLVLLLHGFPDTAWSFVPVM
                     RHLAAAGYRAVAPFMRGYPPSGLAPDGDYGVPALGRDALALIAALGEERAVLVGHDWG
                     AAAAYVAAALEPARISHLVTAAVPHVHRFVYWPTLRQLRRSRYMLFFQLRGLPERRIV
                     AGDFRWLRALIREWSPAWNFGEAEFAPLQQAFSDPARLAAALAYYRAMPRSLFGREGR
                     KLVATRIAVPARVIRGADDGCIGAEMFDNQEQWFSTGYELITLRGAGHFMQCEQPEAF
                     ARAVLGFVR"
     gene            1757..>2081
                     /locus_tag="VHE37_05290"
     CDS             1757..>2081
                     /locus_tag="VHE37_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT34972.1"
                     /translation="MNEFYTQERCWIIELSNSPADEALSIARARVERGVTTRWHRLAG
                     VAERYLMIEGEGRVEVGNAPPRVVRAGDVVLIPAGVPQRIANIGAGELIFLALCTPRY
                     VHSAYE"
BASE COUNT          314 a          689 c          723 g          355 t
ORIGIN      
        1 tgcagttcag ctgagcgcgc tcttcagttc aatgcgcgcc ggcgcggcgg ccagcggcac
       61 gcgcgcctga tcgagcttga gcttgatcgc ctcgagcagc gtggcctcca cgtcgccccg
      121 ctcgggcgtg tgcacccacg gcgacaccag cagttcgacg ccgtcgcggc tgatcgactt
      181 cacgctgacc gccggcgccg gctgcttgag cacgcgcgca tcgccgttga gcacttcggc
      241 cagcagctgc ttggcttgca tcaggtcggc ttcgtgcggc agcggcacgg tgatgtcgat
      301 gcgccgcgta gggcgcgcgg tgaagttggt gatgatgttc gaggtgatcg acgagttggg
      361 cacgatcacc agcttgttgt cgagcgtgcg cagctgcgtg gtgacgatct tgatctcctc
      421 gacgaagccg cgctggccgc cgatctccac gtaatcgccc aggttgaacg ggcggaagat
      481 gatgatgacc acgccggccg cgaacgaggc cagttgcgcc tgcagcgaca ggccgatggc
      541 cagcgccgcg ccaccgagca cggcagcggc cgatgccgta ttaacgccca gctgcgccag
      601 cgcggtgaac accaccacca ccagcgcgag gccatagagc acgtggccga ggaacccggc
      661 cagtgtctcg tccatgcggc cgcggcgcat cagcacgcgc agcagcttca gcagcatctg
      721 tgccagccac ttgccgctga agaagatggc cagcgcggcg atcaggttga acaggccggt
      781 gaccgcgaga tcggccagct tttctccatg cacccaatag ttctgcagca ggttcatcac
      841 gtgggcgagg catgcatccg gggtttcgcg cagtataggc aaggctccac cgggaggcgg
      901 cgatgaacga tccggcgcag atcaggctgc aggccaacgg cgtggatttc tgcgcgctgg
      961 aactgggccg tggtccgctg gtgctgctgc tccacggctt tcctgacacc gcgtggagct
     1021 ttgttccggt gatgcgccat ctggccgctg ccggctatcg cgccgtggcg ccattcatgc
     1081 gcggctatcc gccgagcggt ctggcgcccg acggcgacta cggcgtgccg gcgctgggcc
     1141 gcgatgcgct ggcactgatc gcggcgctcg gtgaagagcg agcggtgctg gtcggccatg
     1201 actggggcgc cgcggctgca tatgtcgccg ccgcgctcga gcccgcgcgc atctcgcacc
     1261 tggtcaccgc agcggtgccg cacgtgcacc gcttcgttta ctggcccacg ctgcgccagc
     1321 tgcggcgatc gcgctacatg ctgttcttcc agctgcgcgg cctgccggag cggcgcatcg
     1381 ttgccggcga tttccgctgg ctgcgcgcgc tgatccgcga atggtcgccg gcgtggaatt
     1441 tcggcgaagc ggaattcgcg ccgctgcagc aggcgttttc cgatcccgcg cgtctggccg
     1501 cggcgctggc ttattaccgt gccatgccgc gcagcctgtt cggccgcgaa ggccgcaagc
     1561 tggtggccac gcgcatcgcg gtgcccgcgc gcgtcatccg cggtgccgac gacggctgca
     1621 tcggcgccga gatgttcgac aaccaggaac aatggttcag caccggctac gagctgatca
     1681 ccctgcgcgg cgccggacat ttcatgcagt gcgagcagcc ggaagcattc gcacgcgcgg
     1741 tgctcgggtt cgtgcgatga atgagttcta cacgcaggag cgctgctgga tcatcgagct
     1801 gtcgaattcg ccggcggatg aagcgctgtc gatcgcgcgg gcacgtgtcg agcgcggtgt
     1861 aaccacgcgc tggcaccggc tggctggcgt cgccgaacgc tacctcatga tcgagggcga
     1921 gggtcgcgtc gaagtgggca acgcgccgcc gcgcgtggtg cgcgcgggcg acgtggtgct
     1981 gattcccgca ggagtgccgc agcgcatcgc caacatcggc gcaggcgaac tgatcttcct
     2041 ggcgctgtgc acgccgcgtt atgtgcactc cgcctacgaa g
//
LOCUS       DASZZC010000248         4804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1114653,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000248 DASZZC010000000
VERSION     DASZZC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114653"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1106
                     /gene="lpxD"
                     /locus_tag="VHE37_05295"
     CDS             39..1106
                     /gene="lpxD"
                     /locus_tag="VHE37_05295"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HVT34973.1"
                     /translation="MTSTPPSYTLAELAERFGLTVHGDAAARISGVCELAPGEAGRVA
                     FLANPKYRAQLSSSQAGAVIVGRRDAAALPGNGLIAAEPHLAYARVARLFDPSDRRVP
                     GIHATASIAPGVRIPHSAQVSAYALIEDGVEIGEEAYIGPHCHVGRGAHIGAGTRLEA
                     RVWIGPRVRIGERGRINPGAVIGARGFGFAPSREGWQEVPQLGTVVIGDDVEVGANTC
                     IDRGALSDTKIGNGVKLDNLIMIAHNVEIGERTAIAACTGIAGSTRIGARCMIGGAAG
                     INGHIEICDDVVLLGRAMVTKSITQKGVYGSGLPLAPVREWHQTVARVRRLHRLEQRL
                     RDIEQQLGIDSKQEQEDASEP"
     gene            1103..1573
                     /gene="fabZ"
                     /locus_tag="VHE37_05300"
     CDS             1103..1573
                     /gene="fabZ"
                     /locus_tag="VHE37_05300"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HVT34974.1"
                     /translation="MIEQTIDIRQILTLLPHRAPFLLVDRVIAHDDTSLTAIKNITYN
                     EPFFPGHFPGVPTTPGVIMLEALAQACGILAVIKSGVRPESGLILYFAGIDNARFKRP
                     VVPGDQLLMKVVIDKHKRDVWKFKAQALVGDELAVEADMMCVLKHPGPVGAAAA"
     gene            1570..2367
                     /gene="lpxA"
                     /locus_tag="VHE37_05305"
     CDS             1570..2367
                     /gene="lpxA"
                     /locus_tag="VHE37_05305"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HVT34975.1"
                     /translation="MSAPKIHPSAEIDGKAQLDGSVEVGPYSVIGAGVQVGAGTVIGP
                     HVVLKGPMTIGRDNQIFQFASLGEISQDKTARPDDPTSVVIGDGNVIREYVSIQRGTM
                     KEYGTKQGITRIGDRNWIMNYCHIAHDCTVGSDTIFANNASLAGHVEIGDHVVLGGYT
                     LVYQWCRLGAHSFSAFSAGISGDVPPFVMVQGNPAEARAINKEGMRRRGFTAADMDAV
                     EAAFKLIYRSGKVMAEVKAELAQMGQTSAHVQAMADFIGSGKRPLQR"
     gene            2367..3539
                     /gene="lpxB"
                     /locus_tag="VHE37_05310"
     CDS             2367..3539
                     /gene="lpxB"
                     /locus_tag="VHE37_05310"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HVT34976.1"
                     /translation="MKFALVAGEISGDILGAALIRALRAKFPAASFYGVAGPRMVEAG
                     CVAIASIEELSIMGIAEVLPALPRLFRLRDALVERFVRDRPDCVIGIDAPDFNLRLER
                     PLRERGLTTVHVVSPTVWAWRQGRVKNIARAVDLILCLFPFEPEFYAGHGVRADYIGH
                     PLADELDAGMTQAQARGALGLDPARRVAAVLPGSRGGELKYLAEPFARAAALLHAHDA
                     SLQFVTPIAKPKLREAMQRAIAAHAPQVPWTLSAGRSREAMLAADAVLLASGTAALEC
                     ALLQRPMVVAYRGSAFTAWLMLRAGLLKTRYVSLPNLLSEKPAVPELLQDAATPERLA
                     QEVRALLDDASARERQLSQFAAVRTELKRDSAALAAQAIARLLDEKNAATAPASRV"
     gene            3625..4263
                     /locus_tag="VHE37_05315"
     CDS             3625..4263
                     /locus_tag="VHE37_05315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HVT34977.1"
                     /translation="MQEFFGDGVGEVENWVVAGVDEAGRGCLAGPVYAAAVIIGKARF
                     RGLDDSKKLSAEAREELFPQIQERVIAWSIARAEVEEIDRFNILRASLLAMQRAVEAL
                     HTAPHEALVDGNVPPPLKCKVTTVVDGDALHRPIMAASILAKVARDREMRRLDGEHPG
                     YGFAQHKGYGTQEHVIALRRLGPCAIHRRSFAPVAQTEFNFDAFPYAVTVEN"
     gene            4265..>4804
                     /locus_tag="VHE37_05320"
     CDS             4265..>4804
                     /locus_tag="VHE37_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HVT34978.1"
                     /translation="MSAAAFVHLRVHTEFSLTDGVVRVEPPKRKGGGTGATLTSRAAE
                     LKLPALAITDRDNLFAMVKFYKAAEKEGIKPIVGADLTLAPLAKNDAPERITLLVQDE
                     QGYRNLTRLISLAYTDGQAKGFAQVAREWLREYSAGLIALTGRDGAVCRAAATDHVDT
                     ALAALGELQDMFPRRLYLEI"
BASE COUNT          801 a         1625 c         1656 g          722 t
ORIGIN      
        1 cggcgggcct tgtcgtttgc gcgggttaaa atgcacgcat gacttccacc ccgccgagct
       61 acacgctggc cgaactggcc gaacgcttcg gcctgaccgt gcacggcgac gccgccgcgc
      121 gcatcagcgg cgtgtgcgag ctggcgccgg gcgaggccgg gcgcgtggcc ttcctggcca
      181 acccgaagta ccgcgcgcag ctgtcgtcga gccaggccgg cgcggtcatc gtcggccgcc
      241 gcgacgccgc cgcgctgccc ggcaacgggc tgatcgccgc cgagccgcac ctggcctacg
      301 cgcgcgtggc gcggctgttc gatccatcgg accggcgcgt gccgggcatc cacgccaccg
      361 cgagcatcgc gccgggcgtg cgcattccgc acagcgcgca ggtgagcgcg tatgcgctga
      421 tcgaggacgg cgtggagatc ggcgaggagg cctacatcgg tccgcattgc cacgtgggcc
      481 gcggcgcgca catcggtgcc ggcacgcggc tggaagcgcg cgtgtggatc ggcccgcgcg
      541 tgcgcatcgg tgaacgcggg cgcatcaatc ccggcgcggt gatcggtgcg cgcggcttcg
      601 gcttcgcgcc gagcagggaa ggctggcagg aagtgccgca gctgggcacg gtggtcatcg
      661 gcgacgatgt cgaggtgggc gccaacacct gcatcgaccg cggcgcgctg tcggacacca
      721 agatcggcaa cggcgtgaag ctcgacaacc tgatcatgat cgcgcacaac gtggagatcg
      781 gcgagcgcac cgcgattgct gcgtgcaccg gcatcgccgg cagtacccgc atcggcgcgc
      841 gctgtatgat cggcggcgct gccggcatca acgggcatat cgagatctgc gacgacgtgg
      901 tgctgctggg ccgcgccatg gtcaccaagt cgatcacgca gaagggcgtc tacggcagcg
      961 ggctgccgct ggcgccggtg cgcgaatggc accagacggt ggcgcgcgtg cgccgcctgc
     1021 accggctgga acaacgcctg cgcgacatcg agcagcaact gggcatcgat tccaagcaag
     1081 agcaagagga cgccagcgaa ccatgatcga gcagaccatc gacatccggc agatcctgac
     1141 gctgctgccg caccgcgcgc cgttcctgct ggtggaccgt gtgatcgcgc atgacgacac
     1201 ctcgctcacc gcgatcaaga acatcaccta caacgagccg ttctttcccg gccattttcc
     1261 cggcgtgccg accacgcccg gcgtgatcat gctggaggcg ctggcgcagg cctgcggcat
     1321 cctggcggtg atcaagtccg gcgtgcgccc ggagagcggg ctgattctgt acttcgccgg
     1381 catcgacaac gcgcgcttca agcgcccggt ggtgccgggc gaccagttgc tgatgaaggt
     1441 ggtgatcgac aagcacaagc gtgacgtgtg gaagttcaag gcgcaggcac tggtcggcga
     1501 tgaactcgca gtggaagccg acatgatgtg cgtgctcaaa cacccgggtc cggtgggcgc
     1561 cgccgcggca tgagcgcgcc aaagatccat cccagcgcgg agatcgatgg caaggcgcag
     1621 ctggacggca gcgtcgaggt cggcccgtac agcgtgatcg gtgccggcgt gcaggtcggc
     1681 gccggtaccg tgatcggtcc gcacgtggtg ctgaaggggc cgatgaccat cggccgcgac
     1741 aaccagattt tccagttcgc ctcgctcggc gaaatctcgc aggacaagac ggccaggccg
     1801 gacgatccca ccagcgtggt gatcggcgac ggcaacgtca tccgcgaata cgtcagcatc
     1861 cagcgcggca ccatgaagga gtacggcacc aagcagggca tcacccgcat cggtgaccgc
     1921 aactggatca tgaactactg ccacatcgcg cacgactgca ccgtcggcag cgacaccatc
     1981 ttcgccaaca acgccagcct ggccggccac gtggagatcg gcgaccacgt ggtgctcggc
     2041 ggctacacgc tggtctacca gtggtgccgc ctgggcgcgc actcgttcag cgcattttcc
     2101 gccggcatct ccggcgacgt gccgccgttc gtcatggtgc agggcaaccc ggccgaggcg
     2161 cgcgccatca acaaggaagg catgcgccgc cgcggcttta ccgctgccga catggacgcg
     2221 gtggaagccg ccttcaagct gatctaccgc agcggcaagg tgatggcgga agtgaaagcc
     2281 gaacttgcgc agatgggcca gacctccgcg cacgtacagg ccatggccga cttcatcggc
     2341 tcgggcaagc ggccgttgca gcgctaatga agttcgccct ggtcgcgggc gagatttccg
     2401 gcgacatcct cggcgccgcg ctgatccgcg cgctgcgcgc gaaatttccg gcagcgagtt
     2461 tctacggcgt ggccgggccg cgcatggtcg aggctggatg cgtagccatc gcctcgatcg
     2521 aggaactgtc gatcatgggc atcgccgaag tgctgccggc actgccgcgg ctgttcaggc
     2581 tgcgcgatgc gctggtcgaa cgcttcgtgc gcgatcgtcc cgactgcgtc atcggcatcg
     2641 acgcgccgga tttcaatctg cggctggagc ggccgctgcg cgagcgcggc ctgaccaccg
     2701 tgcacgtggt cagccccacg gtgtgggcct ggcgccaggg gcgggtgaag aacatcgcgc
     2761 gcgcggtgga cctgatcctg tgcctgttcc cgttcgagcc ggagttctac gccggccacg
     2821 gcgtgcgcgc agactacatc ggccacccgc tggccgacga gctcgacgcc ggcatgacgc
     2881 aggcgcaggc gcgcggcgca cttggcctcg atccggcgcg gcgcgtggcg gcggtgctgc
     2941 cgggctcgcg cggcggcgaa ctcaagtacc tggccgaacc gttcgcgcgc gcggccgcgc
     3001 tgctgcacgc gcacgatgct tcgctgcaat tcgtcacgcc catcgccaag cccaaactgc
     3061 gcgaggcgat gcaacgcgcc atcgctgcgc acgcgccgca ggtgccgtgg acgctgagcg
     3121 caggccgctc gcgcgaggcg atgctcgccg ccgacgcggt gctgctggcc tccggcacgg
     3181 cggcgctgga atgcgcgctg ctgcagcggc cgatggtggt ggcgtatcgc ggctcggcct
     3241 tcaccgcctg gctgatgctc cgcgcgggcc tgctgaagac gcgttacgtc agcctgccga
     3301 acctgctgtc ggaaaaacct gccgtgccgg aactgctgca ggacgccgca acgcccgagc
     3361 gcctcgcgca ggaagtgcgc gcgctgctcg atgatgcgtc agcgcgcgag cgccagctgt
     3421 cgcagttcgc cgccgtccgc accgaactca agcgcgattc cgccgcgctc gctgcccagg
     3481 ccattgcgcg gctgctggat gaaaaaaatg ccgccaccgc accggcatcc cgcgtgtaat
     3541 aaattatcga ctgcatattt ttttgatcga tgttttataa agtggccgca accctaaaat
     3601 gcggctcggt cggcagcatt tttgatgcaa gaattttttg gggacggcgt gggcgaagtt
     3661 gaaaattggg tcgtcgcggg agtggatgag gccggccgcg gctgcctggc cggcccggtc
     3721 tatgccgccg ccgtgatcat cggcaaggcg cgcttccgcg gcctcgacga ttccaagaag
     3781 ctgagtgccg aagcgcgcga ggaactgttc ccgcagatcc aggaacgcgt gatcgcctgg
     3841 agcatcgcgc gggccgaggt ggaagaaatc gaccggttca acatcctgcg cgcttcgctg
     3901 ctggccatgc agcgcgcggt ggaggcgctg cacaccgcgc cgcacgaagc gctggtcgac
     3961 ggcaacgtgc cgccgccgct gaaatgcaag gtgaccacgg tggtcgacgg cgacgcgctg
     4021 caccggccga tcatggcggc gtcgatcctg gccaaggtgg cgcgcgaccg cgagatgcgc
     4081 cggctcgacg gcgaacatcc cggctacggt ttcgcccagc acaagggcta cggcacgcag
     4141 gagcatgtca tcgcactgcg ccggctgggt ccgtgcgcca tccatcgccg gagctttgcg
     4201 ccggtggcac agaccgagtt caacttcgac gcgttcccct acgccgtcac ggtcgagaat
     4261 tgaagtgagc gccgctgcct tcgtgcacct gcgcgtgcac accgagttct cgctcaccga
     4321 cggcgtcgtc cgcgtggagc cgcccaagcg caagggcggc ggcaccggcg ccacgctgac
     4381 ctcgcgcgcg gcggaactga aactgccggc actggccatc accgaccgcg acaacctgtt
     4441 cgccatggtc aagttctaca aggcggcgga gaaggaaggc atcaagccca tcgtcggcgc
     4501 cgacctcacc ctggcgccac tcgccaagaa cgacgcgccc gagcgcatca cgctgctggt
     4561 gcaggacgag cagggctatc gcaatctcac gcggctgatc tcgctggcct ataccgacgg
     4621 ccaggccaag ggctttgcgc aggtggcgcg cgaatggctg cgcgagtaca gcgcgggcct
     4681 gatcgcactc accggacgcg acggcgcggt gtgccgcgcg gccgccaccg atcatgtcga
     4741 caccgcgctg gccgcgctgg gcgaactgca ggacatgttc ccgcgccggc tctacctgga
     4801 gatc
//
LOCUS       DASZZC010000249         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_470646,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000249 DASZZC010000000
VERSION     DASZZC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470646"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VHE37_05325"
     CDS             <1..828
                     /locus_tag="VHE37_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVT34979.1"
                     /translation="VAALAVQDGLAADAQAAQRLLAGKDRAWIAQYLRGRSAAQIMRL
                     PYDQPDLRKKAAGGWNDGQVVAVDARAAFEQGQLAPIPMMIGTTRDEAKLLAGVYRIS
                     PAEMFNLMLHSDPDARPTVRTSDIIAPYMLPSLGSWLYDHYTAAITWALKRGVNQSIA
                     AVAAHDPQVWVYRFDWDRAPEPWHTVYGAAHGIDLPFVFGNFSTGFFAMDFSRANRPG
                     REALSQLMMQSIGAFMRTGDPNTPQLPLRWQQWGGAGKSHAQMIFDASDELPQLTIE"
     gene            1068..>3103
                     /locus_tag="VHE37_05330"
     CDS             1068..>3103
                     /locus_tag="VHE37_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HVT34980.1"
                     /translation="MNHNGNAPRCAMAIFAAFLLAAPRAHAMTILVGSSADDDGANCT
                     LRQAIDSLNAQTLDADCESANEDAFGSNDTVDLSLLALFPGPIEIDPSAVFNIQQSMS
                     LVGPGADVLTLSGSTLFAVPAGSAASYFNVSGLTMTGAAAAPAIQAVNAQTDISITDS
                     LLTGNNAAIVETSGSLTLVRSAVTGNTADTLLSASHTVKLINSTISGNQVSGSLILST
                     ADSVVENSTLIEDSGALVFDTAQPVNLHNSIVIADGAVCSGSAGAGSLPSDVFVSDAS
                     CGTGLHAGIDPALLDLQPLAANGGPSQTIALGAGSLAIGAGDSAICANDDVADMDQRG
                     YERGSAACDAGAYEYAGVRPSIKLPRNLQFASTPIGFIDFRFFKIRNTGAVPLRVKSF
                     DIDGPQAGNFLELGDTCLGASVSPGSYCQVLVAFFPCELGAASARLKVVSEATPRPRM
                     VRLSGTGLLPPPENLQASQGAGKVSLGWSAVDGAASYNVYLQSGTDSPVLALTGVTAT
                     SADVSGLTAGKTYCLMVVAVAGDGTEGGPAFVEVRLPKIPTVSIAFDKTSVYTGAPAV
                     LSFSSADRSSATTCDSSGTWHGSRRTSGKQTISRDRAGTYTYTLTCSNSSGSDTQSAS
                     LTVTDPPPTLSFSSYSLNFVARPVGTRSSTTTVTIYNRGDALQTINNALVLGGA"
BASE COUNT          541 a         1033 c          989 g          540 t
ORIGIN      
        1 gtcgctgcgc tggcggtgca ggacggcctg gctgccgatg ctcaggccgc gcagcgcctg
       61 ctcgccggca aggaccgtgc gtggatcgcg caatacctgc gcggcaggag cgcggcgcag
      121 atcatgcgcc tgccgtacga ccagcccgat ctgcgcaaga aggcggctgg cggctggaat
      181 gacgggcagg tggtggcggt ggatgccagg gccgcgttcg aacaaggcca gcttgcgccg
      241 atccccatga tgatcggcac cacgcgcgac gaggccaagt tgctggccgg cgtctaccgg
      301 atcagtcccg cggagatgtt caacctgatg ctgcatagcg accccgatgc gcggcccacg
      361 gtgcgcacca gcgacatcat tgcgccgtac atgctgccgt cgctcggatc gtggctctac
      421 gatcactaca ccgcggccat cacctgggcg ctcaagcgcg gcgtgaacca gagcattgcc
      481 gccgtggccg cccatgatcc gcaggtctgg gtctaccgct tcgactggga tcgcgcgccg
      541 gagccgtggc acaccgtgta tggcgccgcg catggcatcg acctgccgtt cgtgttcggc
      601 aacttctcga ccgggttctt cgccatggat ttttcgcgcg ccaaccgtcc tggccgggag
      661 gcgctgtcgc agttgatgat gcaatcgatc ggcgcattca tgcgcaccgg cgatcccaac
      721 acgccgcagt tgccgctgcg ctggcagcaa tggggtggcg cgggtaagtc gcatgcgcaa
      781 atgatcttcg acgccagcga cgagttgccg caactcacca tcgaatagcc ggccaaccgc
      841 ggcctgagtc cgcaagcgcg tgtgacccga atcccacccg gcgctcagcc ggagcgtatg
      901 atcgccgggg gcattgagcc cgcaacgcag gatgctccgc gtcggtcagg gaaccgaccc
      961 gggaatcacc acgtagtact tctgctgttg ctgcccgctc gacgtcgcga gccggacgga
     1021 agtgccgctc gcaatgggta cacgtgcatg gttaccgggg atccagaatg aatcacaacg
     1081 gcaacgcgcc gcgttgtgcg atggcaattt tcgcagcctt cctgctcgcg gcgccgcggg
     1141 cgcatgccat gaccatcctg gtcggcagtt ctgccgacga cgatggcgca aactgcacct
     1201 tgcgccaggc catcgacagc ttgaatgccc agaccctcga cgcggactgt gaaagtgcga
     1261 acgaggacgc ttttggatcc aatgacacgg tcgatttgtc cctgcttgcc ttgtttcccg
     1321 gcccgataga aatcgatccg tcggcggtct tcaacatcca gcaatcgatg tcgctggttg
     1381 gccctggcgc cgacgttctc acgctcagcg gcagtacgct gttcgccgtc cctgccggtt
     1441 cggcagccag ctatttcaat gtgagcggcc tgaccatgac cggtgctgcc gcggcgcccg
     1501 cgatccaggc cgtcaacgcg cagacggaca tcagcatcac ggacagcctg ctcaccggaa
     1561 acaacgccgc aatcgtagaa accagtggca gtctcacact ggtccgcagt gcggtcaccg
     1621 gaaacacggc ggacacgctc ttgtcggcca gtcacaccgt caagctgatc aattccacca
     1681 tctccggcaa ccaggtgagc ggttcgctca tcctgagcac ggccgattcc gtcgtcgaaa
     1741 acagcacgct gatcgaggac agcggtgccc tggtcttcga caccgcgcag cccgtcaacc
     1801 tgcataacag catcgtcatt gccgatgggg ccgtctgctc cggcagcgcc ggcgccggca
     1861 gccttccgtc cgatgtgttc gtcagtgatg cgagctgcgg caccgggctg catgccggca
     1921 tcgatccggc cttgcttgat ctgcagccgc tggctgccaa cggcggacct tcgcagacca
     1981 ttgcgctggg ggccggcagt ctggccattg gtgccggtga ttcggccatt tgcgccaacg
     2041 acgatgtggc cgacatggat cagcgcggct atgagcgcgg cagcgcagcc tgcgatgcgg
     2101 gtgcgtacga atacgccggc gtgcggccgt cgatcaagct gcccaggaat ttgcagttcg
     2161 cgtctacgcc gatcggcttc atcgacttcc gtttcttcaa gatccggaac accggtgcag
     2221 tgcccctgcg tgtcaagagc tttgacatcg atggcccgca ggcgggtaac ttcctggaac
     2281 tgggcgacac ctgcctgggc gcgtcggtaa gccccggcag ttactgccag gttctggtcg
     2341 cattcttccc ctgcgaactc ggagcggcca gcgcgaggtt gaaggtcgtc tccgaggcca
     2401 cgccgcgtcc gcggatggtg cggctgagcg gcacggggct gcttccgccg cccgagaatc
     2461 tccaggcaag ccagggcgca ggcaaagtga gcctgggctg gtcggcggtg gatggcgctg
     2521 ccagctacaa cgtctacttg cagagcggta ccgacagccc cgtgctggcc ctgacgggcg
     2581 tgactgcgac gtctgcggat gtatccggtc tgaccgcggg gaagacctat tgcctgatgg
     2641 ttgtcgcggt tgcgggggac ggcacggagg gaggcccggc attcgttgag gtgcggttgc
     2701 cgaaaattcc gacggtgagc atcgccttcg acaagacgtc ggtgtatacc ggcgccccgg
     2761 cagtgctcag cttcagctcc gccgaccgca gctctgcgac cacgtgcgac tcgagcggca
     2821 cctggcatgg ttcccggcgc accagcggca agcagaccat ttcgcgcgac cgtgccggca
     2881 cctacaccta cacgctcacc tgcagcaaca gcagtggcag cgacacccag tcggcaagcc
     2941 tcacggtcac cgatccaccg cccacgctat cgttcagcag ctactcgctg aacttcgtcg
     3001 cacgcccggt cggcacgagg agctcgacga ccacggtcac catctacaac cgcggcgacg
     3061 ccttgcagac catcaacaat gcgctggtgc tgggcggggc caa
//
LOCUS       DASZZC010000250         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1044744,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000250 DASZZC010000000
VERSION     DASZZC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044744"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2366
                     /locus_tag="VHE37_05335"
     CDS             <1..2366
                     /locus_tag="VHE37_05335"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HVT34981.1"
                     /translation="LNLLVAAAYCVSGRLGLLLAIPPGYASAVWPAAGIALAAVMLGG
                     YRLASGIWLGSFLVNVWTAFDGGTLDALMHSLLVPSAIASGAALQAMLGALLIQTLAG
                     YSNLLAQELAVVRILVLGGPVACVINCTVGVGTLVALGRVPLDSALFNWWTWWVGDSM
                     GVLVFTPLILVWSVRPYHQWIWRQLAATLPLVMLFALVVWLFVNANAREHARVNQQLD
                     AMGERFADHLADDIDQATDMLYDEAAFFGASHEVERGEFSYFASRLLQRMPHVLGLAF
                     DRRITDAQRAAFEAQGQAEGLQGFHLREAQPDGSTVPAPVHDEYVVIDYMEPRSGEAA
                     SRGINVAAEPKRRAALLQAANSQRAVASEPLVLVQDPQHAPSLLVFMPVYPGNAVLSD
                     AQQRMQQLMGYAVIILRISDLLATSVKLATDQGADVWVYDDGADAPRRLLYGQGEPPP
                     VGPLYFGRTLHVDCNGRTWRVELRVSAETVLAKPSWQIWTLLAAGMLFTGLVGTFLLV
                     VIGRQSKVEELADRRTAELSRANTDLRREVQRSEKLEVEAQRRARELEASNRELEQFA
                     YVASHDLQAPLRSIASFAQLLQQRYGNKLDADAAEFIGYIRSGVGRMDTMINDILRLS
                     RLAPEKLTLGQVNTQQLVRRACSQLASEIAASGADVRYDNLPEVTADAGMLTQLFQHL
                     LSNAIKFQPKGNKPLITVEAQSHGSEWTFAVRDNGLGIKPQFLSQVFDMFRRLHPQEE
                     YSGSGIGLTVCRKIVQLHRGRIWAESNGTQGTAILFTIPVPVPRGGQ"
     gene            2459..2719
                     /locus_tag="VHE37_05340"
     CDS             2459..2719
                     /locus_tag="VHE37_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34982.1"
                     /translation="MGKSTMAMAAVAAWALACSTQASMARPQGLSAPLAQALANVQAQ
                     QRERMRSDFRTGWAELHRQLAEQQVAQRAPAATESEAVKSAP"
     gene            complement(2834..>3206)
                     /locus_tag="VHE37_05345"
     CDS             complement(2834..>3206)
                     /locus_tag="VHE37_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34983.1"
                     /translation="LIEDFVAGRGGLKGMGLRAGLKMLKAARPGIMDRATERMLPDFL
                     EALDPLFQKFRKAGGKDFAAYLQQHEHEAVKALVHVADTKASDASPGVQSYYAKFRHG
                     SENEVKALIPGLGRLVQHHLK"
BASE COUNT          508 a         1084 c         1108 g          506 t
ORIGIN      
        1 tgctcaatct gctggtggca gcggcctact gcgtcagcgg caggctgggc ctgctgctgg
       61 ccattccccc gggctatgcc agcgcggtat ggccggcggc cggcatcgcg ctcgccgcag
      121 tcatgctggg cggctaccgg ctggcgtcgg gcatctggct gggctccttc ctggtcaacg
      181 tgtggaccgc gttcgacgga ggaacgctcg atgcgctgat gcattcgctg ctggtgccca
      241 gcgccatcgc cagcggtgcc gcgctgcagg ccatgctcgg cgccctgctg atccagaccc
      301 tggcgggcta cagcaacctg ctggcgcagg aactggcggt ggtgcgcatc ctggtgctgg
      361 gcgggccggt ggcctgcgtg atcaattgca ccgtcggcgt cggcacgctg gtggcgctgg
      421 gccgggtgcc gctggacagc gcgctgttca actggtggac ctggtgggtg ggcgattcga
      481 tgggcgtgct ggtattcaca ccgctgatcc tggtgtggag cgtgcgcccg taccaccagt
      541 ggatctggcg ccagctcgct gccacgctgc cgctggtgat gctgttcgcg ctggtggtgt
      601 ggctgttcgt caacgccaat gcgcgcgagc acgcgcgggt gaaccagcag ctcgacgcca
      661 tgggcgagcg cttcgccgac cacctggcgg acgacatcga ccaggccacc gacatgctgt
      721 acgacgaggc ggcgttcttc ggcgcatcgc acgaggtcga gcgcggcgag ttctcctatt
      781 tcgcctcgcg cctgctgcag cgcatgccgc atgtgctcgg cctggcgttc gaccgccgca
      841 tcaccgacgc acagcgtgcc gccttcgagg cgcaggggca ggccgagggc ctgcagggat
      901 ttcatctgcg cgaggcgcag cccgacggca gcaccgtgcc ggcgccggtg cacgacgaat
      961 acgtggtgat cgattacatg gagccgcgca gcggcgaagc ggccagccgg ggcatcaacg
     1021 ttgcggccga acccaagcgc cgcgcggcgc tgctgcaggc ggcgaacagc cagcgcgcgg
     1081 tggccagcga gccgctggtg ctggtgcagg acccgcaaca cgcgcccagc ctgctggtgt
     1141 tcatgccggt atacccgggc aacgcggtgc tgagcgacgc tcagcagcgc atgcagcagc
     1201 tgatgggcta cgcggtcatc atcctgcgca tcagcgacct gctggccacc tcggtcaagc
     1261 tggccaccga ccagggcgcc gacgtctggg tctatgacga tggcgccgac gccccccgcc
     1321 ggctgctgta cgggcagggc gaaccgccgc cggtcggccc gctgtacttt ggccgcaccc
     1381 tgcacgtcga ctgcaacggc cgcacctggc gcgtggaact gcgcgtgtcg gcggagaccg
     1441 tgctggccaa gccttcctgg cagatctgga cgttgcttgc cgccggcatg ctgttcaccg
     1501 ggctggtcgg caccttcctg ctggtggtga tcgggcggca atccaaagtg gaagagctgg
     1561 ccgaccgccg caccgccgag ctgtcgcgcg ccaacaccga cctgcgccgc gaggtgcagc
     1621 gcagcgaaaa gctcgaggtg gaagcgcagc gccgcgcgcg cgaactggag gcgagcaacc
     1681 gcgagctgga gcagttcgcc tacgtcgcct cgcacgacct gcaggcaccg ctgcgcagca
     1741 tcgccagctt cgcccagctg ctgcagcagc gctacggcaa caagctcgac gccgacgctg
     1801 ccgagttcat cggctatatc cgcagcggcg tcggccgcat ggacaccatg atcaacgaca
     1861 tcctgcgcct gtcgcggctg gcgccggaga agctcacgct ggggcaggtc aatacacagc
     1921 agctggtgcg ccgcgcctgc agccagctgg ccagcgagat cgcagccagc ggtgccgacg
     1981 tgcgctacga caacctgccc gaggtcactg ctgatgcggg catgctcacc cagctgttcc
     2041 agcacctgct cagcaacgcc atcaagttcc agcccaaggg caacaagccg ctgatcacgg
     2101 tggaagcgca gtcgcacggg tcggagtgga ccttcgccgt gcgcgacaac ggcctgggca
     2161 tcaagccgca gttcctgtcg caggtgttcg acatgttccg ccggctgcat ccgcaggagg
     2221 aatattccgg cagcggcatc ggcctcaccg tgtgccgcaa gatcgtgcag ctgcaccgcg
     2281 ggcgcatctg ggccgagtcc aacggcaccc agggcaccgc catcctgttc accatcccgg
     2341 tgccggtgcc gcgcggcggc cagtagtgcc acggttttgt tccgcgccgt tgcaaccgtt
     2401 tgcgcgcccc gcgcatccaa ccggcgcagg caagcagccc tgcgcagcgg agggcgtcat
     2461 gggcaagtcg acgatggcaa tggcggcagt ggcggcatgg gcgctggcct gcagcacgca
     2521 ggccagcatg gcgcggccgc aggggctcag tgcgccgctg gcgcaggcgc tggccaacgt
     2581 acaggcgcag cagcgcgagc gcatgcgcag tgatttccgc accggctggg ccgaactgca
     2641 ccggcagctg gcggagcagc aggtggcgca gcgcgcgccg gctgctacgg aatccgaagc
     2701 ggtgaaatcg gcgccgtgaa gcccgcggaa tggcttaccg cacccccgcc tgtcgtgtta
     2761 taaaaaattg tataatgtta caaatagaca ataatatata actggcgaca gcggggtgcg
     2821 gtggggcaaa aaatcacttc aggtggtgct gcaccagccg gcccaggccg gggatcaggg
     2881 ccttcacctc attctcgctg ccgtggcgga acttggcgta gtacgactgc acacccgggc
     2941 tggcgtcgct ggccttggtg tcggccacgt gcaccagcgc cttcaccgcc tcgtgctcgt
     3001 gctgctgcag gtaggcggcg aagtccttgc cgccagcctt gcggaatttc tggaacagcg
     3061 gatcgagcgc ctcgagaaaa tccggcagca tcctctcggt ggcgcggtcc atgatgccgg
     3121 gccgtgccgc cttgagcatc ttcagcccgg cgcgcaggcc catgcccttg aggccgccgc
     3181 gaccggcgac gaagtcctcg atcagc
//
LOCUS       DASZZC010000251         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_470698,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000251 DASZZC010000000
VERSION     DASZZC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470698"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VHE37_05350"
     CDS             <1..582
                     /locus_tag="VHE37_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT34984.1"
                     /translation="YNYNGSKLDSQAVIGAASLVFHELVPGHHLHITLQKNNEQLPPV
                     RKNAFVNAFNEGWAEYAATLTGEMGLYDDPYDLYGRLVMDAFLTSRLVVDTGMNTLGW
                     SWEQAKEYMRVHTLCAEGEIESDTLRYSCGIPAQALAYKLGDEEILRMREKVRARLRQ
                     AFSYRDFHSAVLNAGALPLPVLEWHLDRVFARP"
     gene            complement(629..1366)
                     /locus_tag="VHE37_05355"
     CDS             complement(629..1366)
                     /locus_tag="VHE37_05355"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HVT34985.1"
                     /translation="MDPKEPMADAAIAANVPSAAANSAGLNAADIAALNARYQPLSFE
                     QRLLQLYREFDVGQVLVTSSFAATSAYFLHIISRLAPKQKIAFINTGFHFAETVEYKK
                     FLVGQFGLDVFDVAAEEWKHLFTVDDKTYEKDPDYCCAINKVEPLDAIKPNYKIWMSS
                     LMAWQTDNRAGMGIFEERRGIVKFNPMIDVTRAERDAYIREHGLPFHPLVAQGYSSIG
                     CTHCTVKGEGRSGRWAGKAKTECGLHL"
BASE COUNT          269 a          469 c          492 g          275 t
ORIGIN      
        1 tacaactaca acggctccaa gctcgacagc caggcggtga tcggtgcggc ctcgctggtg
       61 ttccacgagc tggtgcccgg acatcacctg cacatcacgc tgcagaagaa caacgagcag
      121 ctcccgccgg tgcgcaagaa cgcgttcgtc aacgccttca acgaaggctg ggccgagtac
      181 gccgctacgc tcaccggcga gatggggttg tacgacgacc cttacgatct ctacggccgc
      241 ctggtcatgg atgcgtttct caccagccgc ctggtggtgg acaccggcat gaacacgctg
      301 ggctggagct gggagcaggc caaggagtac atgcgggtgc acacgctgtg cgccgagggc
      361 gagatcgaat ccgacacgct gcgctacagc tgcggcattc ctgcgcaggc gctggcctac
      421 aagctgggtg atgaagagat cctgcgcatg cgcgagaaag ttcgcgcccg cctccggcag
      481 gccttcagct accgcgactt ccacagcgcc gtgctcaatg ccggtgcctt gccgctgccg
      541 gtgctggagt ggcacctgga ccgggtcttc gcccggccct agctgcgtgc gtgcgatcct
      601 gcgccgatat cgcgcgccgg gttcgggttc agaggtgcag cccgcactcc gtcttggcct
      661 tgccggccca gcggccgctg cggccttcgc ccttcaccgt gcaatgcgtg cagccgatcg
      721 acgaatagcc ctgcgccacc agcgggtgga acggcaggcc atgctcgcgg atgtaggcgt
      781 cgcgctcggc gcgcgtcacg tcgatcatcg ggttgaattt cacgatgccg cggcgctctt
      841 cgaagatgcc catgccggcg cggttgtcgg tctgccaggc catcaggctc gacatccaga
      901 tcttgtaatt gggcttgatc gcgtccagcg gctccacctt gttgatggcg cagcagtagt
      961 cgggatcctt ctcgtaggtc ttgtcgtcca ccgtgaacag gtgcttccac tcctcggcgg
     1021 cgacatcgaa gacatcaagc ccgaactggc cgacgaggaa cttcttgtac tccaccgttt
     1081 cggcgaaatg gaaaccagtg ttgatgaagg cgatcttctg cttcggcgcc agccgcgaaa
     1141 tgatgtgcag gaaatacgcg ctggtggcgg cgaacgacga ggtgaccagc acctggccca
     1201 cgtcgaactc gcggtacagc tgcagcaggc gctgctcgaa cgacagcggc tggtagcgcg
     1261 cgttgagcgc ggcgatgtcc gcggcgttca ggccggcgga attggcggca gcgctcggta
     1321 cgttcgcggc gatggcggcg tcggccatgg gttccttcgg gtccatgttg cgtgaggcgc
     1381 gcattgtacg gacggcggag gcggaaaaaa cgttaaggac gattaacgtc taaagaaata
     1441 tcgcctatga cttgagctgt ctctgtctca gcagcagata gaaggcgaag ccggcggcca
     1501 cgccc
//
LOCUS       DASZZC010000252         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_399725,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000252 DASZZC010000000
VERSION     DASZZC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399725"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VHE37_05360"
     CDS             complement(<1..816)
                     /locus_tag="VHE37_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34986.1"
                     /translation="MLKQIRCPMPQWLIGLLLALPGLAHADGYSAGAARVDTTPPPFD
                     ATADAAAFPLCPSAVYTGPRRFAFEEPYIDLNGNGRYDYGAVDADGNPDPGDPPEPYC
                     DANANGRWDGIFISGGVDHQAVQVHDPLDARAIAISDGTHALILESIVEQGIFINVID
                     RIRARVAQLRPAVDPDFVIVSANHNEASPDTVGIYGAPDIEGAFGLHSGIDDYYVSFL
                     VEQAAQAAVQAYDHMSAATLWATSFSIPANLTVRLSNNFPTTNDDGTPAAIDPK"
     gene            963..2684
                     /locus_tag="VHE37_05365"
     CDS             963..2684
                     /locus_tag="VHE37_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HVT34987.1"
                     /translation="MNHARLAALLPCLLVAACSPRGQAPAAAAAPTPQALLKHSEEFR
                     RDVVKVSDNVYSATGYGIANSIMLVGNDGVIIVDTMETVEAARQVLAEFRKITDKPVK
                     AVVYTHSHPDHVGGASVFVAGGDVPVYAQEELVPNMDRTATELRPIITARSMRMYGVQ
                     LSPQERPNIGIGPFVAMGGDASVGTVRPTRTFRDEMEDTVAGIHFKLVHAPGETPDQL
                     FMWLPERKVLLCGDNVYRAFPNLYTIRGTGYRSPKAWAASIDKMRALHAEYLVPSHSR
                     PLSGAGQIDQVLTDYRDAIRYVYDQSVRLMNQGLLPDEIAARVHLPAHLASSPFLQEF
                     YGKASWSAKTVFDGNLGWFDGDPAHLQPLAPDDEAQRMVRLVGAAELDAKIAEAEQAG
                     DHQWALQLSGYALRANPQDQKARTLRIAALRALGEAETNPPARDYYLMSAGELAHDFK
                     PIEEAVRPTPEMLKGMPLQLYFDGMAVNLHAEDVLDKTIKVGFDFSDTHEQYSYIVRR
                     GASEVVSGILPDADIVVRVPAQVFKEMLAKLRNPALTIAKEFEVTRGNKLEFVRFMAL
                     FQPPSAG"
     gene            complement(2807..3079)
                     /locus_tag="VHE37_05370"
     CDS             complement(2807..3079)
                     /locus_tag="VHE37_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34988.1"
                     /translation="MRVPKLLLALAAAAALSGCVVAPYDGYYGNGGYYGSPGYGYPVY
                     GGAYPSSSFVFSYHDDHRYHGPGYAPPPYYGHGYHDHDHDGGWWHH"
BASE COUNT          565 a         1048 c         1090 g          536 t
ORIGIN      
        1 cttcggatcg atcgccgccg gcgtgccgtc gtcgttggtg gtcggaaaat tgttcgacag
       61 gcgcaccgta agattggccg ggatgctgaa actggtggcc cacagtgtgg ctgcgctcat
      121 gtggtcgtag gcctggacag cggcctgcgc ggcctgctcg accaggaagc tcacgtagta
      181 atcgtcgatg cccgagtgca ggccgaaggc accctctata tccggcgcgc cgtagatgcc
      241 cacggtgtcc ggcgaggcct cgttgtggtt ggccgagacg atgacgaaat cgggatcgac
      301 cgccgggcgc agctgcgcca cgcgcgcgcg gatgcggtcg atgacgttga tgaagatgcc
      361 ctgctcgacg atgctttcca ggatcagcgc gtgcgtgccg tcggaaatgg cgatggcgcg
      421 cgcgtcgagc ggatcgtgca cctgcaccgc ctggtgatcg acgccgccgg agatgaagat
      481 gccgtcccag cggccgttgg cgttggcatc gcaatacggt tccggcggat cgcccggatc
      541 gggattgccg tcggcgtcga ccgcgccata gtcgtagcgg ccgttgccgt tcagatcgat
      601 atacggctcc tcgaaagcga agcggcgcgg gccggtgtac acggcagacg ggcacagcgg
      661 gaaggccgcc gcatcggcgg tggcatcgaa cggcggcggc gtggtatcga cgcgtgcggc
      721 gccggcgcta tagccatcgg catgcgccag ccccggcagc gccagcagca ggccgatcag
      781 ccattgcggc atcgggcagc ggatttgctt gagcatcgtc tcctcgcttg gtcatgcctg
      841 tctgcgcagg ctgctggata gtaatcaccg cgcgtcgcac caacgctctc tttacatttc
      901 tggcgcaagc accgcggctc ctctatcttt gcgcacgggg agatcaccgg aaagaaataa
      961 aaatgaacca tgcccgcctg gcagcgctgc tgccctgttt gctcgttgcc gcctgcagcc
     1021 cgcgcggcca ggcgcctgcc gccgccgctg cgcccacccc gcaggccctg ctcaagcaca
     1081 gcgaggaatt ccgccgcgac gtggtcaagg tgtccgacaa cgtctactcg gccaccggct
     1141 acggcatcgc caactcgatc atgctggtgg gcaatgacgg cgtgatcatc gtcgacacca
     1201 tggaaacggt ggaggccgcg cgccaggtgc tggcggaatt ccgcaagatc accgacaagc
     1261 cggtgaaggc cgtggtgtac acgcactcgc acccggacca cgtgggcggt gcgtcggttt
     1321 ttgtggccgg cggcgacgtg ccggtgtatg cgcaggaaga gctggtgccg aacatggacc
     1381 gcaccgccac cgagctgcgg ccgatcatca ccgcgcgctc catgcgcatg tatggcgtgc
     1441 agctgtcgcc gcaggagcgc cccaacatcg gcatcgggcc gttcgtggcc atgggcggcg
     1501 acgccagcgt gggcacggtg cggcccacgc gcaccttccg cgacgagatg gaggacaccg
     1561 tcgccggcat ccacttcaag ctggtgcacg cgccgggcga gacgcccgac cagctgttca
     1621 tgtggctgcc ggagcgcaag gtgctgctgt gcggcgacaa cgtctaccgt gcatttccca
     1681 atctctacac catccgcggc accggctacc gctcgccgaa agcgtgggcg gcgtcgatcg
     1741 acaagatgcg cgcgctgcac gccgagtacc tggtgccgag ccactcgcgc ccgctgtccg
     1801 gcgccgggca gatcgaccag gtgctgaccg attaccgcga cgccatccgc tacgtctacg
     1861 accagagcgt gcggctgatg aaccagggcc tgctgccgga cgagatcgcc gcgcgcgtgc
     1921 atctgcccgc gcacctggcg tcatcgccgt tcctgcagga gttctacggc aaggccagct
     1981 ggagcgcgaa gaccgtgttc gacggcaacc tcggctggtt cgacggcgac cccgcgcacc
     2041 tgcagccgct ggcgccggac gacgaggcgc agcgcatggt caggctggtc ggcgcggccg
     2101 aactcgacgc gaagatcgcc gaggccgagc aggccggcga tcaccaatgg gcactgcagc
     2161 tgtcgggtta cgcgctgcgc gccaatccgc aggaccagaa ggcgcgcacg ctgcgcatcg
     2221 ccgcgctgcg cgcgctgggc gaagcggaaa ccaatccgcc ggcgcgcgac tactacctga
     2281 tgtcggccgg cgagctggcg cacgacttca agccgatcga ggaagcggtc cggcccacgc
     2341 cggagatgct gaaaggaatg ccgctgcagc tgtacttcga cggcatggcg gtgaacctgc
     2401 acgcggaaga cgtgctggac aagaccatca aggtcggctt cgacttcagc gacacgcacg
     2461 agcagtacag ctacatcgtg cggcgcggcg cgtcggaagt ggtgagcggc atcctgcccg
     2521 atgccgacat cgtggtgcgc gtgccggcgc aggtgttcaa ggagatgctg gccaagctgc
     2581 gcaacccggc gctgaccatc gccaaggagt tcgaggtgac gcgcggcaac aagctcgagt
     2641 tcgtgcgctt catggcgctg ttccagccgc cgtccgccgg ctgagcgcgc taaaaaaaca
     2701 tccaccgcac ccggtccagt cttgttatta aatgtcgtat atttttataa tttatcgata
     2761 attaataaca agcgatggcc gggtgcggtg actcgttttt ccggcttcag tgatgccacc
     2821 agccgccgtc gtgatcgtgg tcgtgatagc catggccgta gtaaggcggc ggcgcatagc
     2881 ccggcccgtg gtagcggtga tcgtcgtggt agctgaagac gaatgacgac gacgggtagg
     2941 ccccgccata caccgggtag ccgtagcccg gcgagccgta gtagccgccg ttgccgtaat
     3001 aaccatcgta cggcgcaacc acgcagccgc tcagcgcggc ggccgcggcg agtgcaagca
     3061 gcagtttggg gacacgcatg ggactctccg ttttggacga gtccagtgtg ggcgcagccg
     3121 ctgaatccgg actgaacccc cgcggacgag ccgggttcag gctgtggcaa ttttctctac
     3181 ggtgccttcg tagtacagca gctgcccgct gtcgtccttc acgcagcgcg agttctccg
//
LOCUS       DASZZC010000253         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_338434,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000253 DASZZC010000000
VERSION     DASZZC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338434"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..790
                     /locus_tag="VHE37_05375"
     CDS             203..790
                     /locus_tag="VHE37_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HVT34989.1"
                     /translation="MSSYYEILEVSRRATPETIRAAFRSLLQRHHPDKHGACADAQRR
                     TRLLLEAYEVLADEARRRACDARPASSELHAARAAATRPAGSAARFDWRPWLWPALGM
                     GWAVAMSLRLKLALVAMTESLGASWTSGAPCPAGHAAIALLLAAPAMSLITVRRPLLW
                     RVPIIVAGLLAASPAGADCVHGSLDACLLLGPTSR"
     gene            complement(844..1437)
                     /locus_tag="VHE37_05380"
     CDS             complement(844..1437)
                     /locus_tag="VHE37_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34990.1"
                     /translation="MKIRTTIIAAVIGIASGSAFAQDTRAEADVQRDANQQQRIEQGL
                     QSGQLTTREAGKLERDETRVDRMEKRDMRNGSISPAEQARLNAAQHKASADIARDRHN
                     GVTGNPTSASSQRMQADVQRDANQQQRIENGMQSGQLTNREAGKLERGQAHVNRTEAR
                     ASRSGAVNANEQARIEGKENRQSNRVWNKKHNETQKY"
     gene            complement(1844..>2814)
                     /locus_tag="VHE37_05385"
     CDS             complement(1844..>2814)
                     /locus_tag="VHE37_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34991.1"
                     /translation="HLRLQIPSLGIDQTFAGSTREESRDLLKEWFKGAGADQLKQLQT
                     ALAKASPIDPVAGNPNSLMAQMVGADFAVATDALGAGKEGFGGGASDTRNLFGASVRF
                     GRYTQNNFDATVLQLPLSYTHLMDAPGYALSFDLPLTYVDNNGAKSGSASLGVGLRVP
                     VAERWSLIPAVRVGVVASADLGAGSLMYSGGVTSDYQLPSFSTKVNIGLANMVALYKT
                     GSLKVGEYETGYDLQNYVFRNGILLDGEPGWHGFESLLRWEVSLVHTDYTGSDLYSRS
                     STDIGASIGTRRQTGATVWNALRLGATYTFGPGKISGAQVNFGYTF"
BASE COUNT          430 a          930 c          928 g          526 t
ORIGIN      
        1 cgggcgtcat cccggggctt ttttcattgt cacggactct caccagggac ttcgtcccag
       61 ttgcgcgggc gcgcatgcac aaggcggcga agatcccgcc gggtgtaggc aactcttcgt
      121 cctgacggcc tcattgagtg gttttcccct cgccgcctgg acgaccccgc tgcaaggtgc
      181 gcgacctgat tcagcccgcg ccatgtcctc gtactacgaa atactcgaag tgagccgccg
      241 cgccacgccg gagaccatcc gcgcggcgtt caggagcctg ctgcagcggc atcacccgga
      301 caagcacggg gcctgcgcag atgcgcagcg gcgcacgcgc ctgctgctcg aggcctacga
      361 agtgctggcg gatgaagcgc gccggcgcgc ctgcgatgcc cgtcccgcca gctcggaact
      421 gcacgccgcc agggccgcgg cgacccgccc cgccggcagc gcagcgcgtt tcgattggcg
      481 gccgtggctg tggccggcac tgggcatggg atgggcggtg gcgatgtccc tgcgtttgaa
      541 actggcgctg gtcgcaatga cggaatcgct gggcgcatct tggaccagcg gcgcaccttg
      601 tccggccggc catgctgcga tcgccttgct gctcgccgcc cctgcaatgt ccctcatcac
      661 cgtgcgccga ccattgctgt ggcgggtgcc gataatcgtt gcaggcctgc tcgccgcctc
      721 accggcaggt gctgactgcg tgcacggctc gctcgacgcc tgtctcctgc tcggaccgac
      781 cagccgctaa aaaaacaagg ccggcttgcg ccggccctgc tgcttctgac ggagcgaaga
      841 agttcagtac ttctgcgtct cgttgtgctt cttgttccag acgcggttgc tctggcggtt
      901 ttccttgccc tcgatgcgcg cctgctcgtt ggcattcacg gcgccgctgc gcgaggcacg
      961 cgcttcagtg cggttcacgt gcgcctggcc gcgctcgagc ttgccggctt cgcggttggt
     1021 cagctggcca ctctgcatgc cgttctcgat gcgctgctgc tggttggcgt cgcgctgcac
     1081 gtcggcctgc atgcgctgcg acgaggcgga ggtgggattg ccggtcacgc cgttgtgccg
     1141 gtcgcgggca atgtcggcgc tggccttgtg ctgggcagcg ttcaggcgcg cctgctcggc
     1201 ggggctgatg ctgccgttgc gcatgtcgcg cttctccatg cgatcgaccc gcgtctcgtc
     1261 acgttccagc ttgcccgcct cgcgcgtggt caactggccc gactgcaggc cctgttcgat
     1321 gcgctgctgc tggttggcgt cgcgctgcac gtcggcctcg gcccgcgtgt cctgcgcaaa
     1381 tgcgctgccg ctggcaatgc cgatgacggc ggcaatgatg gttgtacgaa ttttcatgtg
     1441 gtcgctcctt gcttcgagcc ggcgaagatg ccgggctttc taaaaggtgc gcaaggcacc
     1501 gtggagcaac actttaggcc tgccgcctga gcggaggctg aatggaaaat gacggattga
     1561 ttgaactttt gaaccgccgg agggcgcact tccgtgtgcg cccgcgctgc ctgccggtag
     1621 gagtagttat ccggctcggc gcgacttcac tctctccccg cacccccaca aaaatcgaat
     1681 cgactcccgc cttgtttcaa agtgcgcgtc ggcgagtagt cgagtggacg aaacagtgcc
     1741 acatgactca gctgtcaggc aatagttgca aacatcctga atagaacgct cgttcaccgg
     1801 ccggtgagcc gcgaatcgac cgcagcagca gcgttgcggg catctagaag gtatagccga
     1861 aattgacctg tgcgccggaa atcttgcccg gcccgaaggt atacgtggcg cccaggcgca
     1921 atgcattcca taccgtggcg ccggtctgcc ggcgcgtgcc gatggaggcg ccgatgtcgg
     1981 tcgaggagcg gctgtacagg tcggagccgg tgtagtcggt gtgcaccagg gagacctccc
     2041 agcgcagcag cgactcgaag ccgtgccagc cgggctcgcc gtcgagcagg atgccgttgc
     2101 ggaacacgta gttctgcagg tcgtaaccgg tctcgtattc gccgaccttg agcgaaccgg
     2161 tcttgtacag cgccaccatg ttggccaggc cgatgttgac cttggtggag aacgaaggca
     2221 gctggtaatc gctggtgacg ccgccggaat acatcagcga gccggcgccc aggtcggccg
     2281 aagccaccac gcccacgcgc accgccggga tcaggctcca gcgctcagcc accggcacgc
     2341 gcaggcccac gcccaggctg gcgctgccgc tcttggcacc gttgttgtcg acgtaagtca
     2401 gcggcaggtc gaacgacagt gcgtaacccg gcgcgtccat caggtgcgtg tacgacagcg
     2461 gcaactgcag cacggtggca tcgaaattgt tctgcgtgta gcggccgaag cgcaccgagg
     2521 cgccgaacag gttcctggtg tcgctggcac cgccgccgaa gccttccttg ccggccccca
     2581 gcgcatcggt agccacggcg aagtccgcgc ccaccatctg cgccatcagg ctgttgggat
     2641 tccccgccac cgggtcgatc ggcgaagcct tggccaatgc cgtctgcagc tgcttgagct
     2701 gatcggcgcc ggcgcccttg aaccattcct tgagcaggtc gcggctctcc tcgcgggtgg
     2761 agccggcgaa ggtctggtcg atgcccagcg aaggaatctg caggcgcagg tgcg
//
LOCUS       DASZZC010000254         8183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_534334,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000254 DASZZC010000000
VERSION     DASZZC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534334"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..937)
                     /locus_tag="VHE37_05390"
     CDS             complement(215..937)
                     /locus_tag="VHE37_05390"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HVT34992.1"
                     /translation="MSATLLLSDLHLPAQPSPLRDAFIAFLEGPARQAAGVYILGDLF
                     EYWIGDDIGLQLYAREVSRLAALSALGVPVHFMRGNRDFLVGRHFALITGATILRDPV
                     IVELGGQPTLLSHGDLWCTQDEKYQHWRRFSRNPVAQWLFLRLPRALRERIAGGVRAR
                     SGANKMDKPAEIMDVDDDAVRAAFPQARVQRIIHGHTHRPARHHYRIGERDCERVVLA
                     DWTTQRMEYLRVDGATIERVPV"
     gene            complement(934..1077)
                     /locus_tag="VHE37_05395"
     CDS             complement(934..1077)
                     /locus_tag="VHE37_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34993.1"
                     /translation="MFLGSLPWYALAVGLNQDGFLVAGFGSELAALALYILSSIVALF
                     TRR"
     gene            complement(1121..1654)
                     /locus_tag="VHE37_05400"
     CDS             complement(1121..1654)
                     /locus_tag="VHE37_05400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT34994.1"
                     /translation="MTTANAANPRVLLETSAGNITLELDAQKAPGTVANFVQYVKDKH
                     YDGLVFHRVIRDFMIQGGGYDEKYKERATRAPIKNEAANGLKNLRGTIAMARTGAPHS
                     ATSQFFINHKDNGFLDHPGQDGWGYCVFGKVVDGMDVVDKIATTQTDAIPPFGRDVPL
                     IPVVIKHATLVDAGKGG"
     gene            complement(1707..2702)
                     /gene="dusA"
                     /locus_tag="VHE37_05405"
     CDS             complement(1707..2702)
                     /gene="dusA"
                     /locus_tag="VHE37_05405"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HVT34995.1"
                     /translation="MSSHSPHRFCVAPMLDWTDRHCRYFFRLMSKHARVYTEMLAAGA
                     VLHGGPRLLKYNKAEHPIALQVGGSDADEMAQCAESAEEFGYDEININCGCPSDRVQE
                     GRFGACLMREPQRVAECVSMMTKSASVPVTVKTRIGIDHSEDYVFVRRFVDIVAEAGC
                     DTFIIHARKAWLQGLSPKENREIPPLNYDVVYRLKQEFPQLTIIINGGIHTFEECDEH
                     LKHVDGVMLGRELYDNPFRLAEVDRHFFGRNMAPLTREQVIDQLKPYIENELRVGTGL
                     SHITRHILGLYRGQPGGRRFRQVLSENAHKLGAGIAVLEAALAEVRPDAPDLIAA"
     gene            2778..3650
                     /locus_tag="VHE37_05410"
     CDS             2778..3650
                     /locus_tag="VHE37_05410"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HVT34996.1"
                     /translation="MIFAVTHSSWTAAVEPAEAARLTAQLEQGGVLRFAALPFAVNDA
                     ERALFDPKILSGARKNISYDLARDRLAGAADDAVTPLLHGMLKRYATQAQALVHALFP
                     HYRGALQPGRSSFRPAEIAGRASSVKKDDTRLHVDAFPATPLQGRRILRVFTNVNPDG
                     KTRHWRVGEPFAQVAERFAQPVDALPGIARRLLRAARITKSYRTAYDDLMLKTHDAMK
                     MDDAYQDSVPREDVHFAPGETWVVYSDQVSHAALAGQHLMEQTFYLPADKMLDPDQSP
                     LRVLERLKGRALLV"
     gene            3664..3849
                     /locus_tag="VHE37_05415"
     CDS             3664..3849
                     /locus_tag="VHE37_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT34997.1"
                     /translation="MSTFPIRGFTSGRFAHDGADRRRALPVSPQEPAAVHDKTEHQGA
                     RILGLRFRGSSISRPNL"
     gene            3886..4143
                     /locus_tag="VHE37_05420"
     CDS             3886..4143
                     /locus_tag="VHE37_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HVT34998.1"
                     /translation="MHHVLDVPEDLQSHHTAITSGYIIEGHVPAEDITRLLLEKPDLR
                     GIAVPGIQGASPGMEDFSNDRRPYRVVAFDRDSRISSFAAH"
     gene            complement(4197..4931)
                     /locus_tag="VHE37_05425"
     CDS             complement(4197..4931)
                     /locus_tag="VHE37_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HVT34999.1"
                     /translation="MVIVAVAVAAFCATYAGGLFALSLKDRLHLVLGFSAGSVVAVAF
                     FDLIPESIELGRAFHAPTTLLSWVALGFLLYLILDRVHVLRGHKLHRHANDGGHSIER
                     GIFGAASLSAHSFLDGIVIGVAFQASASVGAVVAVAVLTHDFSDGINTANIVLKNGGD
                     RRQVVRWLLADAIAPAAGMGCAALIIVPPPVLGIVLALFGGFFLYIGASDLIPESHHA
                     HPKLLTTATTVLGAAVLYLTTIFVNS"
     gene            complement(4990..5361)
                     /locus_tag="VHE37_05430"
     CDS             complement(4990..5361)
                     /locus_tag="VHE37_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVT35000.1"
                     /translation="MGARILRILHRRARPMSAYALLGELRRENPLNLYPQTIYRALGD
                     LIEKGFVHKLSSVNAFLACTHPRGTHEGIHLICDDCGMATELVDARVDALLNRKAGAL
                     AFKTQYRAVELHGVCGKCSQA"
     gene            5511..5831
                     /locus_tag="VHE37_05435"
     CDS             5511..5831
                     /locus_tag="VHE37_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HVT35001.1"
                     /translation="MSPIQANPGDDKVTRRHFLQVLCAIAIFATQLAAVFHATQHELA
                     NAGGVHCELCEVAHAAPVPPSPVLVPVVPRFDIVLAPVAPAGPEDRRPFSRPHSRAPP
                     RVLA"
     gene            5951..6589
                     /locus_tag="VHE37_05440"
     CDS             5951..6589
                     /locus_tag="VHE37_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT35002.1"
                     /translation="MLAIHQLEFQYPQSAKIRVPDVNLGQGDQCLVLGPSGCGKTTLL
                     HLAAGLLKPSSGSVRLSGADLTGMDGTELDRFRGRNIGIVFQRLHLIPSLTVLQNLTV
                     AQYCAGLAIDLERARQALAKLGVSNKAEALPAHLSQGQSQRVAIARAVVNQPQLILAD
                     EPTSSLDDANAQRVLELLIEQAKLCNAVLLIATHDARAKAVIQRRVELGAPA"
     gene            6586..7842
                     /locus_tag="VHE37_05445"
     CDS             6586..7842
                     /locus_tag="VHE37_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT35003.1"
                     /translation="MNLLTLALADLREQKLANALHLLLLMLGVAMVTALLLIGNQIGG
                     RFERDAHGVDLVIGAKGSPLQLILSTVYHLDVPTGNIPLAEASRWGHHPMIASAIPIS
                     LGDNARGFRIVGTTPELIRNYQASVVAGREWSGPMEAVIGADVAKAGLELNATFIGVH
                     GLSAAGHAHADHPYKVVGVLAPTNTVLDRLILTSLDSVWEVHSQHHHHEPATAGAEPD
                     ADGDDDDDHEAAAADGYQPSKEITALLVRYRSPLAAAALPRMINTNSNLQAAVPAFET
                     ARLLQLLGLGFDTLRALAIVLIAAATLSVFVALYNAMEARQYDVAVLRSLGASRREVV
                     TLLWLEALMLSAAGTILGILLAHAAVELLGHVAGTAKGVRMTGWDWLPGEMLILLLPL
                     AVGTVAAALPAWRAYRTDVAHTLSRG"
BASE COUNT         1443 a         2630 c         2649 g         1461 t
ORIGIN      
        1 ccttcgacgc actgctgcgc gaacacaaca tgcgcgtgcg ccagccgatc aaccgcgacg
       61 cgcggctgcg cgccggcttc agtgcagcgg aaatggatcg gtgggaatgc gaaaaaaggt
      121 ccaccgcacc cctcgataga cggttataaa ttattgtttt aaaatttaaa caataaataa
      181 tttataacat gtcgaatgcc ggtgcggtgc agcctcaaac cggcacgcgc tcgatggtcg
      241 cgccgtcgac gcgcaggtat tccatgcgct gcgtcgtcca gtcggccagc acgacgcgct
      301 cgcaatcacg ttcgccgatg cggtagtggt gccgcgccgg ccggtgggtg tggccgtgga
      361 tgatccgctg cacgcgcgcc tgcggaaacg ccgcgcgcac ggcgtcgtcg tccacgtcca
      421 tgatctctgc cggcttgtcc atcttgttcg caccgctgcg tgcgcgcacg ccgccggcga
      481 tgcgctcgcg caatgcgcgc ggcagtcgca ggaacagcca ctgcgcgacg gggttgcgcg
      541 agaagcggcg ccagtgctga tatttttcgt cctgggtgca ccagaggtcg ccgtgcgaca
      601 gcagcgtcgg ctggccgcca agctcgacga tcaccgggtc gcgcaggatc gtcgcgccgg
      661 tgatcagtgc gaaatgcctg ccgaccagga agtcgcggtt gccgcgcatg aagtgcaccg
      721 ggacgccgag tgcgctgaga gccgccagcc ggctgacttc acgggcgtag agctgcaggc
      781 caatgtcgtc gccgatccag tactcgaaca ggtcgccgag gatatagacg ccggccgcct
      841 gccgcgccgg gccttcgaga aatgcgatga aggcgtcgcg cagcggcgag ggctgcgcgg
      901 gcaggtgcag gtcggacagc agcagcgtgg cgctcatcgg cgcgtgaaca gcgcaacgat
      961 gctggacagg atgtagagcg cgagcgcggc cagttcgctg ccgaagccgg cgaccaggaa
     1021 gccgtcctgg ttcagcccca ccgccagtgc gtaccagggc agcgagccga ggaacacgat
     1081 cttggcggcc agccggagcc gcgcggcaag tcgcgccagc tcagccgccc ttgccagcgt
     1141 cgaccagcgt ggcgtgcttg atcaccaccg ggatcagcgg cacgtcgcgg ccgaacggcg
     1201 ggatggcgtc ggtctgcgtg gtggcgatct tgtcgaccac gtccatgccg tcgaccacct
     1261 tgccgaacac gcaatagccc cagccatcct gtcccgggtg gtcgaggaag ccgttgtcct
     1321 tgtggttgat gaaaaactgc gaggtggccg agtgcggcgc accggtgcgc gccatggcga
     1381 tagtgccgcg caggttcttc aggccgttgg cggcctcgtt cttgatcggc gcgcgcgtgg
     1441 ctcgctcctt gtacttttcg tcgtagccgc cgccctggat catgaaatcg cggatcacgc
     1501 ggtggaacac caggccgtcg tagtgcttgt ccttcacgta ctggacgaag ttggccacgg
     1561 tgcccggcgc tttctgcgcg tcgagctcga gcgtgatgtt gccggcgctg gtttcgagca
     1621 gcacgcgggg gttggcggca ttggcagtgg tcatggcgag tccgagcaga aaggtgagca
     1681 gcagtttttt catcggtgaa gattcctcag gcagcgatca ggtcaggagc gtccggccgc
     1741 acttcggcca gcgcggcttc cagcaccgcg atgccggcgc ccagcttgtg cgcgttctcg
     1801 ctcagcacct ggcggaagcg gcgcccgccg ggctggccgc ggtacaggcc caggatgtgg
     1861 cgggtgatgt gcgacagccc ggtgccgacg cgcagttcgt tctcgatgta cggcttgagc
     1921 tggtcgatca cctgctcgcg ggtcagcggc gccatgtttc ggccgaagaa gtggcggtcc
     1981 acctcggcca ggcggaacgg gttgtcgtac agctcgcggc cgagcatcac gccatccacg
     2041 tgcttgaggt gctcgtcgca ttcctcgaag gtgtggatgc cgccgttgat gatgatggtc
     2101 agctgcggga attcctgctt gagccggtac accacgtcgt agttgagcgg cgggatctcg
     2161 cggttttcct tgggcgacag cccctgcagc caggccttgc gcgcgtggat gataaaggtg
     2221 tcgcagccgg cctcggcgac gatgtcgacg aagcgccgca cgaacacgta gtcctcggaa
     2281 tggtcgatgc cgatgcgcgt cttcaccgtc accggcacgc tggccgactt ggtcatcatc
     2341 gacacgcatt cggccacgcg ctgcggctcg cgcatcaggc aggcgccgaa gcggccttcc
     2401 tgcacgcggt ccgacgggca gccgcagttg atgttgatct cgtcgtagcc aaattcctcg
     2461 gcggattccg cgcactgcgc catctcgtcg gcatcggagc cgccgacctg cagcgcgatg
     2521 ggatgctcgg ccttgttgta cttcagcagg cgcgggccac cgtgcaatac ggcgccggca
     2581 gcgagcattt ccgtatagac gcgcgcatgt ttcgacatca gccggaagaa ataccggcaa
     2641 tggcggtcgg tccagtccag catcggcgcg acgcagaagc ggtgagggga gtgggaactc
     2701 atcttcgccc ggggcgtgac gcgccatgcg cgccggccct atcatccttt tttgtctgag
     2761 cagttgcgcg caattatatg atttttgccg tcactcattc gagttggact gccgccgtcg
     2821 agccggccga ggccgcgcgc ctgacggcgc agctggagca gggtggcgtg ctgcgcttcg
     2881 ccgcgctgcc gttcgccgtg aacgatgcgg aacgtgcgct gttcgatccg aagatcctct
     2941 ccggcgcgcg caagaacatc agctacgacc tggcgcgcga ccgcctggcc ggcgcggctg
     3001 acgacgcggt cacgccgctg ctgcacggca tgctcaagcg ctatgccacg caggcgcagg
     3061 cgctggtgca cgcgctgttc ccgcattacc gcggcgcgct gcagccgggg cgcagcagct
     3121 tccgcccggc ggagatcgcc ggccgcgcca gttccgtcaa gaaggacgac acgcgcctgc
     3181 acgtcgacgc ctttcccgcc acgccgctgc agggccgccg catcctgcgc gtgttcacca
     3241 acgtgaaccc ggatggcaag acgcggcact ggcgcgtggg tgagccgttt gcgcaggtcg
     3301 ccgaacgttt cgcccagccc gtcgatgcgc tgccgggcat cgctcggcgc ctgctgcgcg
     3361 cggcgcgcat caccaagagc tatcgcaccg cgtacgacga tttgatgctg aaaacgcacg
     3421 acgccatgaa gatggacgac gcctaccagg acagcgtgcc gcgcgaagac gtgcacttcg
     3481 cgccgggcga aacctgggtg gtgtacagcg accaggtgtc gcatgccgcg ctcgccggcc
     3541 agcacctgat ggagcagacg ttctatttgc cggcggacaa gatgctcgac ccggaccagt
     3601 cgccgctgcg cgtgctggag cggctcaagg ggcgggcact gctggtgtga cgacgcatca
     3661 cgcgtgagca cgttcccgat tcggggtttc accagcgggc gctttgcgca tgacggcgcg
     3721 gaccggcgcc gcgcgctgcc cgtgtcgccg caggaaccgg cggcggtgca cgacaagacc
     3781 gagcaccagg gcgcgcgcat cctcggcctg cgcttcaggg gctcatcgat aagtaggcca
     3841 aatttgtaac ggtttagcgt ggtgcatgac ccggacgtgt ccgtcatgca ccatgtcctg
     3901 gacgttccag aagacctcca gtctcaccac acggcaatca cttcgggtta catcatcgaa
     3961 gggcatgttc cggcagagga catcacgcgc ctgctgcttg agaaacccga cctcagggga
     4021 atcgctgtcc ctggcatcca gggcgcttct ccggggatgg aagacttcag caatgaccgc
     4081 cgtccgtatc gggtggtggc cttcgaccga gacagccgga tcagcagctt tgccgcgcac
     4141 tgaagaattg cgatctccgc ttcgcgtgag attcgtgatt tatcgcgcga cgtcggttac
     4201 gaattgacaa agatcgttgt cagataaaga accgccgctc ccagcaccgt cgtcgctgtg
     4261 gtgagcagct tggggtgtgc gtggtgactc tccgggatca agtcgcttgc gccgatgtac
     4321 agaaagaatc cgccgaacag ggcgaggaca atgcccagga ccgggggcgg aacgataatc
     4381 agcgcggcgc atcccatgcc ggcagccggg gcgattgcat cggccagcag ccagcgcaca
     4441 acttggcgcc gatctccgcc gttcttgaga acgatgttcg cggtgttgat gccgtcagag
     4501 aaatcgtgag tcagcaccgc gacagccacg accgctccga ccgaagccga cgcctgaaac
     4561 gccaccccga ttacgatgcc gtccagaaaa ctatgagcag agagactggc cgcgccgaag
     4621 attccgcgct cgattgaatg tcctccgtca ttggcgtgcc ggtgcagctt gtgaccacgc
     4681 aggacatgaa cccgatcaag gatcaaatag aggaggaacc ccagcgcgac ccatgacaac
     4741 agggtcgtcg gggcatggaa cgcccggccc agttcgatgg actcaggaat aaggtcaaag
     4801 aaggcgaccg caaccaccga gccggcgctg aagccaagga ccaggtgcaa ccggtctttt
     4861 agcgagagcg caaacaagcc tccagcatag gtggcgcaaa atgcggcgac cgcaacggcc
     4921 acgatcacca tgcttgccac cggcgctcgc ctctgcaggt gctttgcgga aaatcgggca
     4981 tccgtacctt catgcttggc tgcatttgcc gcaaactccg tgcaattcga ctgcgcggta
     5041 ctgtgtcttg aaggcgaggg cgcccgcttt cctgttgagt agtgcgtcga cccgcgcatc
     5101 caccagttcc gtagccatgc cgcagtcgtc gcagatcaga tggatgccct cgtgggtgcc
     5161 gcgcgggtga gtgcaggcga gaaatgcgtt aacactgctg agcttgtgca cgaaaccctt
     5221 ttcgatcaga tcgccgagcg ctcgatagat cgtctgagga taaaggttca gagggttttc
     5281 gcggcgcaat tcaccgagca aggcataggc cgacatcggc cgggcgcgcc gatgcaggat
     5341 gcgcaggatg cgtgcgccca ccgaagcggg ctccagaccc gactctgcga atgcgctgcg
     5401 tggtcgatgc tccgtgctcc tgcctgtcat ctggatattc cgcatttcaa gccaagccga
     5461 gcggcttatg ttacgttata accgcggccc gccttcgcgc gtgctgctcg atgagcccga
     5521 ttcaggcgaa cccaggtgat gacaaggtga cgcggcggca cttccttcag gtcttgtgtg
     5581 caatcgcaat tttcgcgacg caactggccg ccgtgttcca tgcaacgcag cacgagttgg
     5641 ccaatgccgg cggcgtgcac tgcgagttgt gcgaagtggc gcatgccgcg ccggtgccgc
     5701 catccccggt tctggtcccc gtcgtccctc gctttgatat cgtcctggcc cccgtagccc
     5761 ctgccgggcc ggaagatcgc cgtccatttt cgcgcccgca cagccgggct ccaccgcgcg
     5821 tcctcgccta gcgtctcctg accataagtt cgacgctgcc gcaaagggcg tcgccggtcg
     5881 ttcaccgagg atgggtcatg cgcattggcg aaaccgcgaa tgctcatgac gtgcgtggag
     5941 tggcgtcgtc gtgcttgcga ttcaccagct tgaattccaa tacccgcagt cggcaaagat
     6001 ccgcgtgccc gacgtcaatc tcggtcaagg cgaccagtgc ctcgtgctgg gcccttcggg
     6061 ttgcggcaag accacgttgc ttcatctcgc ggcagggctg ctgaagccga gttcaggatc
     6121 ggtgcgcctg tccggcgcag acttgactgg catggatggc acggagctgg accgctttcg
     6181 cggccgcaac attggcatcg tgttccagcg cctgcacctg attccgtccc tgacggtgct
     6241 gcagaacctg acagtggcgc agtattgcgc cggcctggcg atcgacctgg agcgcgctcg
     6301 ccaggcactg gcaaagcttg gtgtcagcaa caaggccgag gcgctgccgg cgcatctgag
     6361 ccaagggcaa tcgcagcgcg ttgcgattgc ccgcgccgtg gtgaatcaac cgcaactgat
     6421 tcttgccgac gagccgacgt cgagcctcga cgatgcgaat gcgcagcggg tgctcgagct
     6481 cctgatcgag caggccaagc tatgcaacgc cgtgctgctg atcgccaccc acgatgcccg
     6541 ggccaaggcc gtgatccagc gccgcgttga gctgggagca ccggcatgaa tctcctgact
     6601 ctggccctgg ctgacctgcg cgagcagaag ctcgcgaacg cgctgcattt gcttctattg
     6661 atgctgggtg tagccatggt caccgcgctg ctgctgattg gcaatcagat cggcggtcgc
     6721 ttcgagcgcg acgcgcatgg cgtcgacctg gtgatcggtg ccaagggcag cccgctgcag
     6781 ctgatcctgt caactgtcta ccacctcgac gttccgaccg ggaatattcc actggccgaa
     6841 gccagccgct ggggccatca cccgatgatt gccagtgcaa tcccgatctc gctcggcgac
     6901 aacgcgcgcg gcttccgcat cgtcggcacc acacctgaac tgatacgtaa ctaccaggcg
     6961 tcagtcgttg cggggcggga atggtccggg ccgatggaag ctgtcatcgg tgccgatgtc
     7021 gccaaagcgg ggctcgaact caacgcaaca ttcattggtg tgcatgggct gtcggcagcc
     7081 gggcacgctc atgccgatca tccttacaag gttgtcggcg tgctggcgcc gaccaataca
     7141 gtgctggacc ggttgatcct gaccagcctc gacagcgttt gggaagtcca ttcgcagcat
     7201 catcaccacg aacctgccac tgccggcgcc gagccggatg cggacggcga cgacgatgat
     7261 gaccatgagg cagctgccgc agacggctac cagccatcga aggaaatcac tgcactcctg
     7321 gtccgctacc gctcgccgct ggcagcggca gcgctgccgc gcatgatcaa tacaaactca
     7381 aacctgcagg cggctgtgcc ggccttcgag acagcgcgcc ttcttcaatt gctggggctg
     7441 ggcttcgata cgctgcgcgc gctggcaatc gtgctgatcg cagccgccac gctatcggtt
     7501 ttcgtcgccc tctacaacgc catggaagcc aggcaatacg atgtcgcggt cttgcgcagc
     7561 ctgggcgcgt cgcgtcgcga agtcgtgacg ctgctctggc ttgaagccct gatgctcagc
     7621 gctgccggga caattctcgg gattcttctg gcgcacgcag ccgtcgaatt gctggggcat
     7681 gtcgcgggca cggccaaggg cgttcgcatg accggatggg actggcttcc cggcgagatg
     7741 ctgatcctgc tgctgccgct tgccgtgggg accgtggctg cggcgctgcc cgcgtggcgt
     7801 gcctaccgca ccgacgtcgc ccacacactg tcccgaggct agcgatgcac cgccaggccc
     7861 tgctgctgtg tctcgtgtgc gccattggcg gatgccatcc gtcacccgct cccgctgcat
     7921 ccattgcacc aaccaacccc gcggatacca aaattccacc aggccccccg aggcctccgc
     7981 gcgcgacgag ttccctgaag ggagcgcctg tcgcggcagc gccgtccacc agcttcagcc
     8041 agggttcgcc agcgctggcg gcgaggaaga agagcgtcgt gagttaccag aacggcaaac
     8101 tggtcaagga tggctcggcc ccggtcatcc agattgcctc ggagtttgaa aactacgtcc
     8161 acggcaagct cgacgtacca gcc
//
LOCUS       DASZZC010000255         4845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_662648,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000255 DASZZC010000000
VERSION     DASZZC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662648"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /locus_tag="VHE37_05450"
     CDS             <1..2460
                     /locus_tag="VHE37_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35004.1"
                     /translation="ESVAAPAAEAAPGATAGPAATIPVAQPPPAQPAEAETPTDPGVP
                     RIDSIVVTAEKREERLQDVPVSMSVINQEQLERANITQVTDLNRAAPSIEVNGEPGNA
                     DTRISIRGISTNSFSVTAEQAVSLVVDGVVLGKAPSVSLFDIARVEILRGPQGTLFGK
                     NASAGVVSIITNAPDPSRFSIGGHVDLGSKFGYRLAQASVNLPITDDLAIRINAGQTY
                     TKGFIHNVVRNEDSKQDISGARARILWKPLDDFSLNLIADYEKQYTSEQMYIQFEDNN
                     DPATGQPQPLMGCPHSTGGPYAGQDNRISCGNNPTYNDGHDWGLSAQLEYDLNGYILT
                     SISSLRRYAQFDQIDVDGVNGNYYDNSNTFNNKVITQELRIASPVDDRLKWVTGLFYS
                     KSHVPNHLTQTIGSDILLGLGEGEIQSPPVGLPFTLPVPGVPCAQLNICTFDFASLNQ
                     PNDYDAEIKSAAAFGQLTFRLFDPLRLIAGLRYTHDDVAMTSVSYIGVATGNPLIPDT
                     PTIPQNAPLLGRDVVNNLSWRTGLQYDLAKDSMAYLTVSHGYKGPQIVFVPPNDVPGL
                     SGTQVQLPTPAQINHVKPEYPMDYELGIKSTLFDGLLATNADVFYTKIKDFQSSVFNG
                     QGKFTPDNIPSVVTQGVELDVFGYLWEGLTLNSGLLYNKVTYPHPYFVNCTQVGPKCP
                     DTKDGTAEDIGGKQLVIAPRWKVTFSPEYSFKLIGETRGFITSDIVYRSPMHFTDADD
                     TRTELGSHTIIGARLGLRGADDNWEISLFGRNLTNQRNPAFLFAPYLLGSVTQPTTEN
                     SAHALSTESLRFFGVSIGARY"
     gene            2482..3414
                     /locus_tag="VHE37_05455"
     CDS             2482..3414
                     /locus_tag="VHE37_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35005.1"
                     /translation="MKMLSIAALAASLLSTAAVAADAPAAPAASTASARINVWPGVAP
                     GSETWTQQEQDTKMPWGDRVTRNIAKPTLEFYAADPARASGAAMIVAPGGAFRFLSID
                     SEGTQVCSWLAAHGISAFLLRYRTAETAQSDVLFAGQIFAILAPMFAPGPALMDNMKQ
                     YGPPAIADGKQSLRLVRERAAEWKIDAKRVGFIGFSAGGAVATALSLDADASARPNVT
                     AAIYPGPWPLDHVPADAPPLFIAAAADDSITKYGATPLAAAWTAAGRPLDIHLYEKGG
                     HGFGMKTQNKPSDQWLADFAAWLTAQGMITPTAK"
     gene            3416..>4845
                     /locus_tag="VHE37_05460"
     CDS             3416..>4845
                     /locus_tag="VHE37_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HVT35006.1"
                     /translation="MRARHGLLAATLILSSCAGMGTHTQGRHDAPYELRTEYAREPLG
                     IDAAQPRLAWRLPSAVQTAYRVRVATDAGALESAPLWDSGRVVSADSTKLAYAGPALA
                     SRTRYWWQVQTWDANGQASAWSAPSWWETGLLAPADWSASWISGRTAPDHQWSDAKIA
                     VDFTLSGKSLGVLWRARPVGKTYGEAYLWRIADEGGRIRLAEQVRRYAGGNSSKVDTV
                     TLRTLSVADDAAQWKQQRHHLRIEAQGKTLKTTLDDLALDTLEDGAQAAGTVGFVASE
                     ASAATIHALSVEGEGAAAFHTEFAQGDNPFTGGSVGQDGLNVASGVPDKDIVLPIAAP
                     APLLRREFALDDQPVSARLYVAAGGLPRLLLNGAPVGEALADGYTDYSKRVLVRTYDV
                     TALLHAGANAIGAELGRGWYGVTEPNEWYWHMAPWHGAPVLRAQLEVTLASGKRVIVG
                     SDAQWRASDGPTLHDSVYGGERYDARL"
BASE COUNT          840 a         1709 c         1592 g          704 t
ORIGIN      
        1 gaatccgtag cggcgccagc ggcggaggct gctcccggcg cgacggccgg accggccgcc
       61 accatcccgg tggcgcagcc gccaccggcg cagcccgccg aggcggagac gcccaccgac
      121 ccgggcgtgc cgcgcatcga cagcatcgtg gtcaccgcgg aaaagcgcga ggagcgcctg
      181 caggacgtgc cggtatcgat gtcggtgatc aaccaggagc agctggagcg cgccaacatc
      241 acccaggtca ccgacctcaa ccgcgcggcg ccgtcgatcg aggtcaacgg cgaaccgggc
      301 aacgccgaca cccgcatcag catccgcggc atctctacca actcgttctc ggtcaccgcc
      361 gagcaggcgg tgtcgctggt ggtggacggc gtcgtgctgg gcaaggcgcc gtcggtgagc
      421 ctgttcgaca tcgcccgcgt ggagatcctg cgcggcccgc agggcacgct gttcggcaag
      481 aacgcgtcgg ccggcgtggt cagcatcatc accaacgcgc cggacccgtc gcgcttctcc
      541 atcggcggcc atgtcgacct cggttccaag ttcggctacc ggctggcgca ggccagcgtc
      601 aacctgccga tcaccgacga cctggccatc cgcatcaacg ccggccagac ctacaccaag
      661 ggtttcatcc acaacgtggt gcgcaacgag gacagcaagc aggacatcag cggcgcccgc
      721 gcgcgcatcc tgtggaagcc gctcgacgac ttcagcctga acctgatcgc cgattacgag
      781 aagcagtaca ccagcgagca gatgtacatc cagttcgagg acaacaacga ccccgccacc
      841 ggccagccgc agccgctgat gggctgcccg cacagcaccg gcgggccata cgccggccag
      901 gacaaccgca tcagctgcgg caacaacccg acctacaacg acggccacga ctggggcctg
      961 tcggcgcagc tggaatacga cctcaacggc tacatcctca cctcgatcag ctcgctgcgc
     1021 cgctacgcgc agttcgacca gatcgacgtc gatggcgtca acggcaacta ctacgacaac
     1081 tcgaacacct tcaacaacaa ggtcatcacg caggaactgc gcatcgcctc gccagtggac
     1141 gatcgcctga aatgggtgac cggcctgttc tactcgaagt cgcacgtgcc caatcacctg
     1201 acccagacca tcggctccga catcctgctg ggcctgggcg agggcgagat ccagtcgccg
     1261 ccggtcggcc tgccgttcac cctgccggtg ccgggcgtgc cctgcgcgca gctcaacatc
     1321 tgcacgttcg atttcgcctc gctcaaccag cccaacgact acgacgccga gatcaagagc
     1381 gccgcggcct tcggccagct cacctttcgc ctgttcgacc cgctgcggct gatcgccggc
     1441 ctgcgctaca cgcacgacga cgtggcgatg acctcggtgt cgtacatcgg cgtggccacc
     1501 ggaaacccgc tgatccccga cacgccgacc attccgcaga atgcgccgct gctcgggcgc
     1561 gacgtggtca acaacctgtc ctggcgcacc ggcctgcagt acgacctggc caaggacagc
     1621 atggcctacc tcaccgtgtc gcacggctac aaggggccgc agatcgtgtt cgtgccgccc
     1681 aatgacgttc ccggcctgtc cggcacgcag gtgcagctgc ccacaccggc gcagatcaac
     1741 cacgtgaagc ccgaataccc gatggattac gagctgggca tcaagtcgac gctgttcgac
     1801 gggctgctgg ccaccaatgc cgatgtgttc tacaccaaga tcaaggattt ccagtcctcg
     1861 gtgttcaacg gccagggcaa gttcacgccc gacaacattc caagcgtggt cacgcagggc
     1921 gtggagctcg acgtgttcgg ctacctgtgg gaagggctca cgctcaacag cggcctgctg
     1981 tacaacaagg tcacctaccc gcatccatat ttcgtcaact gcacgcaggt cgggcccaag
     2041 tgtcctgaca ccaaggacgg caccgcggag gatatcggcg gcaagcagct ggtgattgcg
     2101 ccgcgctgga aggtcacctt ctcgccggag tattccttca agctgatcgg cgagacgcgc
     2161 ggcttcatca ccagcgacat cgtctaccgc tcgccgatgc atttcaccga cgccgacgac
     2221 acgcgcaccg agctggggtc gcacaccatc atcggagcgc ggctgggcct gcgcggcgcc
     2281 gacgacaact gggaaatctc gctgttcggc cgcaacctga ccaaccagcg caatcccgcc
     2341 ttcctgttcg cgccgtacct gctgggcagc gtgacccagc cgaccaccga gaactcggcg
     2401 cacgcgctgt ccaccgaatc gctgcgcttc ttcggtgttt cgatcggcgc gcgctactga
     2461 agtcttcctg gagagatacc gatgaagatg ctgtccatcg ccgcgctggc ggcgtcgttg
     2521 ttgtcgaccg ccgccgtcgc tgcggatgcg cccgctgcgc ctgcggcttc gactgccagc
     2581 gcgcgcatca acgtgtggcc gggcgtggcg cccggctcgg aaacctggac ccagcaggaa
     2641 caggacacca agatgccctg gggcgatcgc gtcacgcgca acatcgccaa gccgacgctc
     2701 gagttctacg cggccgaccc ggccagggcc agcggcgcgg cgatgattgt cgcgccgggc
     2761 ggcgccttcc gcttcctgtc gatcgacagc gagggcacgc aggtgtgcag ctggctggcc
     2821 gcgcacggca tcagcgcctt cctgctgcgc taccgcacgg cggagacggc gcagtccgac
     2881 gtgctgtttg ccgggcagat cttcgccatc ctcgcgccga tgttcgcgcc gggcccggcg
     2941 ctgatggaca acatgaagca gtacggcccg ccggcgatcg ccgacggcaa gcagtcgctc
     3001 aggctggtcc gcgagcgcgc ggccgagtgg aagatcgatg cgaagcgcgt cggcttcatc
     3061 ggcttctccg ccggcggcgc ggtggcgacg gcgctctcgc tcgatgcgga tgcgtcggcg
     3121 cggccgaacg tcacggcggc catctatccg ggcccgtggc cgctggatca cgtgccggcg
     3181 gatgcgccgc cgctgttcat tgccgccgcg gcggacgact cgatcaccaa gtacggcgcc
     3241 acgccgctgg ccgccgcctg gaccgcggcc ggccggccgc tggacattca cctgtatgaa
     3301 aaaggcggcc acggtttcgg catgaagacg cagaacaagc cgagcgacca gtggctcgcg
     3361 gacttcgcag cctggctcac cgcccagggc atgatcacgc ccacggcgaa gtgacatgcg
     3421 cgcgcgccac gggttgctgg cggcgacgct gatcctctcc tcctgcgccg gcatgggcac
     3481 gcacacgcaa gggcgtcacg acgcgccata cgagttgcgc actgaatatg cgcgagagcc
     3541 gctgggtatc gatgcggcac agccgcgcct ggcctggcgc cttccgtccg cggtgcagac
     3601 tgcgtatcgc gtgcgcgtgg cgacagacgc gggcgcactt gaatcggcgc cgctgtggga
     3661 cagcggacga gtcgtctccg ccgacagcac gaagctcgca tacgccgggc ctgcgctggc
     3721 gtcgcgcacg cgctattggt ggcaggtgca gacctgggac gcgaatggac aggcttcggc
     3781 gtggagcgcg ccgtcgtggt gggagaccgg attgctggcg ccggccgact ggagcgcgtc
     3841 gtggatctcg ggccgcaccg cgcccgatca ccagtggagc gacgcgaaga tcgcagtcga
     3901 tttcacgctc agcggaaaat cgcttggcgt gctgtggcgc gcgcggccgg tcggcaagac
     3961 ttatggcgaa gcatacctgt ggcgcatcgc tgacgaaggc ggccgcatcc gcctggccga
     4021 gcaggtgcgg cgctacgccg gcggcaacag ctcaaaggtc gacacggtca cgctgcgcac
     4081 gctgtccgtc gccgacgatg ccgcgcaatg gaagcagcag cgccatcacc tgcgcatcga
     4141 ggcacagggc aagacgctga agaccacgct cgatgacctg gcgctcgata cgctcgaaga
     4201 cggtgcgcag gcggcgggca cggtgggctt cgtcgccagc gaagccagtg ccgcgaccat
     4261 ccatgcgctg agcgtggaag gcgagggcgc ggcggcattc catacggagt ttgcgcaggg
     4321 cgacaacccg ttcaccggcg gctcggtggg ccaggacggc ttgaacgtcg cctcgggcgt
     4381 gccggacaag gacatcgtgc tgcccatcgc cgcgccggcg ccgttgctgc gccgcgagtt
     4441 tgcgctcgac gaccagccgg tgtcggcgcg gctgtacgtc gccgccggcg gcctgccgcg
     4501 cctgctgctc aacggcgcgc cggtcggtga agcgctggcc gacggctaca ccgactacag
     4561 caagcgcgtg ctggtgcgca cctacgacgt caccgcgctg ctgcacgcgg gcgccaacgc
     4621 catcggcgcc gagctcgggc gcggctggta cggcgtgacc gaacccaacg agtggtactg
     4681 gcacatggcg ccatggcacg gcgcgccggt gctgcgcgcg cagctggaag tgacgctggc
     4741 cagcggcaag cgcgtaatcg tcggcagcga cgcgcagtgg cgcgccagcg acggccccac
     4801 gctgcacgat tcggtctacg gcggcgagcg ctacgacgcg cgcct
//
LOCUS       DASZZC010000256         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_787950,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000256 DASZZC010000000
VERSION     DASZZC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787950"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VHE37_05465"
     CDS             <1..393
                     /locus_tag="VHE37_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA (cytidine-2'-O-)-methyltransferase"
                     /protein_id="HVT35007.1"
                     /translation="EPRTLIFYEASHRIRECVAAIAEAFGRERRLCLARELTKLYEES
                     ITAGAADILQWLDADVHRTKGEFVLVCAGAPAQDPSAAQAERLLNILLAELPGSQAAR
                     LAAQISGRRKNELYELALRLVQGADRQS"
     gene            complement(468..1820)
                     /locus_tag="VHE37_05470"
     CDS             complement(468..1820)
                     /locus_tag="VHE37_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT35008.1"
                     /translation="MDASSMSLGRRIALGAGDLGFNLYWQMASLYLLFFYTDVVRLPP
                     ATAGAIYMGALIWDAALDPAIGAFADRTRTRWGRYRPYLLLGGVPLALVYTLVFAGSF
                     GNGNTAVALTAAIHVLFRTVYAVVSIPYAALFARVTHDARLRASLAGYRMIFATLAAV
                     IVAAATLPLVQALSTPEAPRRGWIVVGFASGLLSTLLLLGVAWATRGYDAVEDETPPP
                     HHWRDMLRTLAANRALLLLLGSIMISLFSGTLFGKNLLYYFKYVVGQEKLGSAALAMT
                     ALMVGLFVPVFTWLSRRRGKREAWIIGAWPGLAGLLLWHFADGHGVPLLFGALTLSGI
                     ASAAGAVCFWSLLPDTVEYGEWKTGIRTESLVFGLAILGQKAALGLGAGLLGILLGHA
                     GYVADVPQSPATLDAIKQMMFWIPLAGGVLSLALIWLCPLTLASHAQMVKEIAQRKGG
                     "
     gene            1914..>3472
                     /locus_tag="VHE37_05475"
     CDS             1914..>3472
                     /locus_tag="VHE37_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HVT35009.1"
                     /translation="MSPLWVNIRGNRFVDANGRQLILRGVNLGGDGKLPYPDGGTDRP
                     GDFSDHATVSFVGRPFPLAQADEHLGRIAGWGFNLLRLVVTWEAVEHAGPWRYDTDYI
                     EYIGAVCERAAAYGLAVFIDFHQDVWSRMSGGSGAPCWIFDALGLDYRRFGEADAAHV
                     MQYRYDYRDPRRLQEERYPTMSWPVNYRMAVNGIVWTAFFGGRTFTPQWMLDGVNVQD
                     FLQDHYLGALRALAQRVRELPNVIGFDSLNEPGQGWIGRAMTQRAGIGASGNFRGPAW
                     TPLDGLAIASGLPRSVPFLVPNEAKTKIIGRRDVLMNKHEISIWKDGVADPYAQAGAW
                     SLEHGEPRAADEDFFRKRDGRAFDIERDFMAPFFARVAQAMRSVRADWLLFAEINPHV
                     LVEGRTFPQQMPDRSVNANHWYDVNLLMGKTYPVQMSAAERAQMRKGYAFQLSYLKLM
                     GDRLGGGAPTLVGEFGTPYDLDDGASFKRWAAGERGAVLWQAQANALSLAYDALDELL
                     LSSAQWNYTAS"
BASE COUNT          637 a         1159 c         1147 g          529 t
ORIGIN      
        1 gagccgcgca cgctgatttt ttacgaagcc agccaccgca tccgcgagtg cgtggccgcc
       61 atcgccgaag cattcggccg cgagcgccgg ctgtgcctcg cgcgcgagtt gaccaagcta
      121 tacgaggaaa gcatcactgc aggcgccgcc gacattctgc agtggctcga tgccgatgtg
      181 catcggacca agggtgaatt cgtgctggtc tgtgcaggcg cgccggcgca ggatccgtcc
      241 gccgctcaag ctgagcggtt gctgaatatc ctgctggcgg aactgcccgg ctcgcaggcg
      301 gcgcggctgg cggcgcaaat cagcgggcgg cgtaagaacg aactctatga actggcgctg
      361 cgcctcgtgc agggcgctga tcggcaatct tagattccgc tttcatcttg ctggcggcac
      421 caccatcgca cgccccggaa ggggcactgc cttcgtcgcg aaaatcttca cccacccttg
      481 cgctgtgcaa tctccttcac catctgcgca tgcgaggcca gcgtcagcgg acacaaccag
      541 atcagcgcca gcgacagcac tccaccggcc agcggaatcc agaacatcat ctgcttgatc
      601 gcatcgagcg tggccggcga ttgcgggacg tcggccacgt agccggcatg gccgagcagg
      661 atgccgagca ggccggcccc caatcccagc gcggctttct ggccgaggat ggccagacca
      721 aatacgaggg actcggtgcg tatgccggtc ttccattcgc cgtattcaac ggtgtcgggc
      781 agcagcgacc agaagcacac cgcgccggcg gcggaagcga tgcccgacag ggtcaacgca
      841 ccgaacagca gcggcacgcc gtggccatcg gcgaaatgcc acagcagcag gccggcaaga
      901 ccgggccagg cgccgatgat ccaggcctcg cgcttcccac gcctgcgcga cagccaggtg
      961 aacaccggta cgaacaggcc gaccatcagc gcggtcatgg ccagcgcggc gctgcccagt
     1021 ttctcctggc cgaccacgta cttgaagtag tacagcaggt tcttgccgaa cagcgtgccg
     1081 gagaacaaag agatcatgat cgagccgagg agcagcagca gcgcgcggtt ggcagccagc
     1141 gtgcgcagca tgtcgcgcca gtgatgcggc ggcggcgtct cgtcctccac tgcgtcataa
     1201 ccgcgcgtgg cccacgctac gcccagcaac agcaacgtgc tgagcagccc ggaagcgaat
     1261 ccgacgacga tccatccgcg ccgcggcgct tccggtgtgg acagggcctg caccagcggc
     1321 agcgtggctg ccgcgacgat cacggcagcc agcgtggcga agatcatgcg gtagccggcc
     1381 agggatgcgc gcaggcgcgc gtcgtgcgtc acccgcgcga acagcgcggc gtaaggaatc
     1441 gacaccaccg catacaccgt gcggaacagc acgtggatgg ccgccgtcag cgccacggcg
     1501 gtgttgccat tgccgaacga gccggcgaat accagcgtgt agaccagtgc cagcggcacg
     1561 ccgccgagca gcaggtacgg acgatagcgg ccccagcgcg tgcgggtgcg gtcggcaaaa
     1621 gcgccgatcg cggggtccag cgcggcatcc cagatcagcg cacccatgta gatggcgccg
     1681 gcagtcgctg gcggcaggcg caccacgtcg gtatagaaga acagcagata gagcgaggcc
     1741 atctgccagt aaagattgaa gcccaggtcg cccgcgccca gcgcgatgcg ccggcccagg
     1801 ctcatgctcg atgcgtccat ccgcgtctcc cctgcgctgt ccatcttgcg cgattttgat
     1861 tcaccaactg tataatgaaa ccaacataac acatcactgc acctggagag agcatgtcgc
     1921 cgctgtgggt caacatccgt ggcaaccgct tcgtcgatgc gaacgggcgc cagctcatcc
     1981 tgcgcggcgt caacctgggc ggcgacggca agctgcccta ccccgatggc ggcaccgacc
     2041 gcccgggtga tttttccgat catgccacgg tgagcttcgt cggacggccg ttcccgctgg
     2101 cgcaggccga cgagcacctg ggccgcatcg ccggctgggg tttcaacctg ctgcggctgg
     2161 tggtgacctg ggaagcggtg gagcatgccg gcccatggcg ctacgacacc gactacatcg
     2221 agtacatcgg cgcggtgtgc gagcgcgcgg ctgcgtatgg tctggcggtg ttcatcgatt
     2281 tccaccagga cgtgtggagc cgcatgagcg gcggcagcgg cgcaccgtgc tggatcttcg
     2341 atgcgctggg cctggactac cgccgcttcg gcgaggccga cgccgcgcac gtcatgcaat
     2401 accgctacga ctaccgcgat ccgcgtcggc tccaggaaga gcgttacccc accatgagtt
     2461 ggccggtgaa ctaccgcatg gcggtcaacg gcatcgtgtg gaccgcgttc ttcggcgggc
     2521 gcaccttcac gccgcagtgg atgctcgacg gcgtcaacgt gcaggacttc ctgcaggatc
     2581 actacctcgg cgccctgcgc gcgctggcgc agcgcgtgcg tgaactgccc aatgtgatcg
     2641 gcttcgacag cctgaacgag ccgggccagg gctggatcgg ccgcgcgatg acgcagcgtg
     2701 ccggcatcgg cgccagcggc aatttccgcg ggccggcatg gacgccgctc gacgggctcg
     2761 ctatcgccag cggcctgccg cgcagcgtgc cgttcctggt gcccaacgag gcgaagacca
     2821 agatcatcgg caggcgcgac gtgctgatga acaagcatga aatttccatc tggaaggatg
     2881 gcgtggccga cccgtacgcg caggccggcg cctggagcct ggagcatgga gagccacgcg
     2941 cagccgacga ggacttcttc cgcaagcgcg atggccgcgc attcgacatt gagcgcgatt
     3001 tcatggcgcc gttcttcgcc cgcgtggcgc aggccatgcg cagcgtgcgc gccgactggc
     3061 tgctgttcgc cgagatcaat ccgcatgtct tggtcgaggg acgcactttt ccgcagcaga
     3121 tgcccgaccg cagcgtcaat gccaaccact ggtacgacgt gaacctgctg atgggcaaga
     3181 cctaccccgt gcagatgagc gcggccgagc gtgcacagat gcgcaagggc tacgccttcc
     3241 agctcagcta cctcaagctg atgggcgatc gcctgggcgg cggcgcaccg acgctggtcg
     3301 gcgaattcgg cacgccgtac gacctagacg acggcgcctc gttcaagcgc tgggccgccg
     3361 gcgagcgcgg cgcggtgttg tggcaggcgc aggccaatgc gctgtcgctg gcctacgatg
     3421 cgctcgacga actgctgctg tcgtcagccc agtggaacta caccgcgagc aa
//
LOCUS       DASZZC010000257         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_471403,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000257 DASZZC010000000
VERSION     DASZZC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471403"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VHE37_05480"
     CDS             <1..688
                     /locus_tag="VHE37_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HVT35010.1"
                     /translation="LGPRQGFPHTPMLPHNMAMTVTGAGMLWVGWFGFNGGSALAAGG
                     AAAMAMLVTHISAAAGACAWTAIEWYQYRKPSALGVVTGMVAGLGTITPASGTVGPGG
                     ALLIGLIAGSVCFAATHLMKRVFKVDDSLDVFPVHGVGGITGTLLTGVFTSSTLGAFS
                     GAGYAAGQTMGGQFAVQALGVISTGLYTALVTLALLKLVGALTPLRVSTEQEHAGLDI
                     TEHNETGYNS"
     gene            complement(813..1607)
                     /locus_tag="VHE37_05485"
     CDS             complement(813..1607)
                     /locus_tag="VHE37_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35011.1"
                     /translation="MSGAVLAVKLLASPLLIGLASIGGRRWGPNVAGLLGGLPLVGGP
                     VIAALWMQGGPALAQQVALAAPAGLWANMVYFLVVGYTSARLRWWAAIPLGWCCYLLA
                     ALAVSATGLAYSLPAGLAVIPGLWLTATRALPQPRVAATRVHLPRAELYARMAAAALM
                     VATLSSAAMLIGPGLTGVLSGAPVAAVVIPAFTFAKAGRDALLHALRGFLTGLTGYAA
                     FCLVLGHGAVAPGAWVWLPALAAAVLFGLTATHWSRPVFDLVEEDL"
BASE COUNT          276 a          607 c          585 g          238 t
ORIGIN      
        1 gctcgggccg cgccagggtt ttccgcacac gccgatgctg ccgcacaaca tggcgatgac
       61 ggtgacgggc gccggcatgc tgtgggtggg ctggttcggc ttcaacggcg gctcggcgct
      121 ggccgccggc ggcgccgcgg cgatggccat gctggtcacg catatctcgg ccgcggcggg
      181 cgcttgcgcc tggaccgcga tcgagtggta ccagtaccgc aagcccagcg cgctgggcgt
      241 ggtcaccggc atggtcgccg gtctcggcac catcacgccg gcctcgggca ccgtcggccc
      301 cggcggcgcg ctgctgatcg gcctgatcgc cggctcggtg tgcttcgccg ccacgcacct
      361 gatgaagcgc gtgttcaagg tggacgactc gctcgacgtg ttcccggtgc acggcgtggg
      421 cggcatcacc ggcacgctgc tcaccggcgt cttcaccagt tccacgctgg gcgcattcag
      481 cggcgccggc tacgcggcag gccagaccat gggtgggcaa ttcgcggtgc aggcgctggg
      541 cgtgatttcc actgggctct acaccgcgct ggtcacgctc gcactgctca agctggtcgg
      601 cgcgctgacg ccgctgcgcg tgagcacgga gcaggagcac gccggcctgg acatcaccga
      661 gcacaacgag accggctaca actcctgaaa aattcgttga ccgcaccgcc ggttttgatg
      721 ttatttattg ttgttcgatt ttataaccat aagatcattt ataacatcgg ccagaggggt
      781 gcggtgggtg tcctgctacg aaaaaagtag catcagaggt cttcctccac caggtcgaac
      841 accggccgcg accagtgcgt cgcggtcagg ccgaacagca cggccgcggc gagcgccggc
      901 agccacaccc acgcaccggg cgcgaccgcg ccgtgcccca gcaccaggca gaacgcggcg
      961 tagccggtca ggccggtgag aaatccgcgc agcgcgtgca gcagcgcatc gcgcccggcc
     1021 ttggcgaagg tgaacgccgg gatcaccacc gcggccaccg gcgcgccgga cagcacgccg
     1081 gtgaggcccg ggccaatcag catggccgcg ctcgacaagg tggcgaccat cagcgctgcg
     1141 gccgccatgc gcgcatacag ctcggcgcgc ggcaggtgca cgcgcgtggc cgcaacgcgc
     1201 ggctgcggca gcgcgcgcgt ggcggtgagc cacaggcccg ggatcacggc gaggccggcg
     1261 ggcagcgaat acgccaggcc cgtggcactg accgccagcg ccgccagcag gtaacagcac
     1321 cagccgagcg ggatcgcggc ccaccagcgc agccgcgcgc tggtgtagcc gacgaccagg
     1381 aagtaaacca tgtttgccca cagcccggcc ggcgctgcca gcgccacctg ctgcgccagc
     1441 gccggtccgc cctgcatcca caaggccgcg atcaccggtc cgccgaccag cggcagaccg
     1501 ccgagcaggc ccgccacgtt gggcccccag cgccggccgc cgatgctggc caggccgatc
     1561 agcagcggcg acgccagcag cttgaccgcc agtaccgcgc cgctcacgcc ttgagcgagg
     1621 ccaggtcgat gacgaagcgg tatttcacgt cgctctgcac catgcgctcg taggcctggt
     1681 tgatcttctg catcgggatc atctcg
//
LOCUS       DASZZC010000258         5660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_83356,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000258 DASZZC010000000
VERSION     DASZZC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83356"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="VHE37_05490"
     CDS             complement(<1..1771)
                     /locus_tag="VHE37_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HVT35012.1"
                     /translation="MSSSLYSSLAWLPPAPADFKARCRAAATPAELRALAGFSLDENQ
                     LNSLAKAVERVRSAGTDLKPLSAFRLGLISNATSHFAVPALIASALRHGIALECIEAE
                     YDQGMQEALDPASAINAFKPDAVLLAFDYRGLPLRVVTGDGAAAQQVVSGALAHLQRI
                     RDGIHANSGALCILQTLPRPAETVFGSLDPVVPGTLRALIDAVNRGIGESLAGTPDLL
                     FDVAALAETVGLADWHDPTVWNIAKLPFAGAQLPLYAEQVCRIVAALRGKSRKALVLD
                     LDNTLWGGVIGDDGLEGIVLGQGDGTGEAHLHVQRVALTLRDRGVVLAVSSKNEDATA
                     RLPFQKHPEMLLREEHIAVFQANWNDKAANLKAIAKELNIGTDALVFLDDNPAERTLV
                     RQMLPEVAVPELPEDPALYARTLLAAGYFEAITYSAEDRARADFYRDNAQRVALQQQA
                     GGDIEAYLASLQMTLTLAPFDAIGRARIAQLINKSNQYNLTTRRYTEAEVERMESDPA
                     CYTLQARLADSFGDNGMISVVIARRAGDDWELDTWLMSCRVLQRRVEHAILAQLVSAA
                     RARGIKRLLGTWLPTEKNMMVQDH"
     gene            complement(1768..2010)
                     /locus_tag="VHE37_05495"
     CDS             complement(1768..2010)
                     /locus_tag="VHE37_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVT35013.1"
                     /translation="MENADIYAKLTDVLRDVFDNDAITATPELTAKQVKGWDSLAHIR
                     VVLSVEKAFRVKFSAAEVTKLKNVGDFANLIASKLK"
     gene            2175..2945
                     /locus_tag="VHE37_05500"
     CDS             2175..2945
                     /locus_tag="VHE37_05500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HVT35014.1"
                     /translation="MTPDAIAQRYLDLLARTLTGTLYEEEPNHDTANQGEFVVAFTLH
                     YLRGSAVSMQPKVRLDNIRDCVKQVLADNVAGDFMETGVWRGGGCIYARAALDVYGGG
                     ERTVWVCDSFEGMPKPDAEKHPGEAKFFDSPMMQDMYNRLEATLPDVTRNFEAFQLLS
                     DKVKFVKGWFKDTMPTVPVQQLAILRLDGDYYDSTMDVLKHMYGKVSPGGYVIVDDYG
                     ETGWANCRGATDDFRRDQNISAPLIKVDSKCWYWRKPF"
     gene            complement(2953..3693)
                     /locus_tag="VHE37_05505"
     CDS             complement(2953..3693)
                     /locus_tag="VHE37_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35015.1"
                     /translation="MPNALTQNPAVAAPLWRGRVLFRLMLEDHTDFFLGLQRWRFRHS
                     TFRDNIVDRNTQVVIDGFPRSSNSFSVRAFSGAQRTPVRVGHHVHSHAQILKAVELGI
                     PTVLLVRDPDQCIPSLKALNIQVSPAMEQIVSLEVYLRQYIRFHERVRPVLDRCVVAS
                     FEQTIADFGAVIDRLNARFGTQFDRYQHTAESQDRVFRSNTGHLSPSAQRDNIKQRLR
                     DEYFSAANQPLVERARALYAEVSAAAQR"
     gene            3755..5032
                     /gene="dctA"
                     /locus_tag="VHE37_05510"
     CDS             3755..5032
                     /gene="dctA"
                     /locus_tag="VHE37_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HVT35016.1"
                     /translation="MTRGGRLYLWVLAAIVLGGICGAIDPAGAVKLKPLSDGFIALIK
                     MLIGPVIFLTVVLGIAGIDSVKKVGRIGLKAVVYFELLSTVALALGLVIVNALRPGAG
                     FNADAATLDAGAVAEYSKKAAEQSTADFLLHIIPKTFSDAFSGGGDLLQVLLVAVLFA
                     FALSVIGEQGRPVIALLEGLSKVFFGMVALVMRLAPVGAGAAMAFTIGKYGVAALAPL
                     AKLMAGFYATCLIFIFAVLGMVCRLCGFSIVKFIRYIGAELLLVLGTSSSESALVPLM
                     EKLERAGCARSVVGLVIPAGYSFNLDGTNIYLTMAAIFVAQALNIELTLAQQLSLLLV
                     AMLTSKGAAGVTGSGFITLAATLAVVPSVPVAGLALILGIDRFMSEARALTNIIGNGV
                     ATIVVAKWEGELDARKLAAELQPAATAPSSSLL"
     gene            complement(5116..>5660)
                     /locus_tag="VHE37_05515"
     CDS             complement(5116..>5660)
                     /locus_tag="VHE37_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT35017.1"
                     /translation="AAEFYPMNSDIAARAGVHHALIKYHYHSKEQLWRAAVTFLFHRQ
                     AGDLTFDPPPGAMATPEGRREHAREILRRIVRYSAQHPEHARLMVQESVRDSKRLRWL
                     ADTFVTHTSRAALNYVRLLKQEGMVPDVSESALVYIIVGAAQQFYTLAPEVKRVWKID
                     PSKPAVIDAHVDAMLKVLLR"
BASE COUNT          909 a         1808 c         1949 g          994 t
ORIGIN      
        1 agtgatcctg caccatcatg ttcttctcgg tcggcagcca ggtgccgagc aggcgcttga
       61 tgccgcgcgc acgggctgca ctgaccagct gcgcgaggat ggcgtgctcc acgcgacgct
      121 gcagcacgcg gcaggacatc agccaggtgt cgagctccca gtcatcgccc gctcgacgcg
      181 cgatgaccac gctgatcatg ccgttgtcgc cgaagctgtc cgccagccgc gcctgcagcg
      241 tgtagcaggc cggatcgctt tccatgcgct caacctcggc ctcggtgtag cggcgcgtgg
      301 tcaggttgta ctggttggac ttgttgatca gctgcgcgat acgcgcgcgg ccgatggcat
      361 cgaatggcgc cagcgtcagc gtcatctgca gcgaggcaag gtaagcctcg atgtcgccgc
      421 cggcctgctg ctgcagcgcc acgcgctgtg cgttgtcgcg gtagaaatcg gcgcgcgcgc
      481 ggtcttccgc ggagtaggtg atggcctcga agtaacccgc ggccagcagc gtgcgagcat
      541 acagcgcggg gtcctcgggc aattccggca ccgccacttc tggcagcatc tgccgcacca
      601 gcgtgcgctc ggcgggattg tcgtcaagga acaccagcgc gtcggtgccg atgttgagtt
      661 ccttcgcgat cgccttgagg ttggccgcct tgtcgttcca gttggcctgg aacacggcaa
      721 tgtgttcctc gcgcagcagc atctccgggt gtttctggaa cggcaggcgc gcggtagcgt
      781 cctcgttctt cgacgatacc gccagcacga cgccgcggtc gcgcagggtc agcgccacgc
      841 gctgcacgtg cagatgcgcc tcgccggtgc cgtcgccctg tccgagcacg atgccttcca
      901 ggccgtcgtc gccgatcacg ccgccccaga gggtgttgtc gagatccagc accagtgcct
      961 tgcggctctt gccgcgcagc gcggcgacga tgcggcacac ctgctcggcg tacagcggca
     1021 gctgcgcgcc ggcgaagggc agcttggcga tgttccacac ggtggggtcg tgccagtcgg
     1081 ccaggcccac ggtttccgcc agcgcggcca cgtcgaacag caggtccggt gtgcctgcaa
     1141 ggctctcgcc gatgcctcgg ttcaccgcgt cgatcagcgc gcgcagcgta ccgggcacca
     1201 ccggatcgag gctgccgaac acggtctcgg ccggtcgcgg cagcgtctgc aggatgcaca
     1261 gcgcaccgct gttggcgtgg atgccgtcgc ggatgcgctg caggtgcgcc agcgcgccgg
     1321 ataccacctg ctgcgcggcc gcgccatcgc cggtcaccac gcgcagcgga agcccgcggt
     1381 agtcgaaggc cagcagcacg gcgtcgggct tgaacgcgtt gatggcggaa gcagggtcga
     1441 gcgcctcctg cattccctgg tcgtactcgg cctcgatgca ttccagcgcg atgccgtgac
     1501 gcagcgcgct ggcgatgagc gcggggacgg cgaaatgcga ggtggcgttg ctgatcagcc
     1561 cgagcctgaa cgcggacagc ggcttgagat cagtgccagc ggagcgtacg cgctccacgg
     1621 ccttggccag cgagttgagc tggttctcgt cgagagaaaa accggccaga gcgcgcagct
     1681 cggcaggcgt ggcggcggcg cggcagcgcg ccttgaaatc ggccggcgcc ggcggcagcc
     1741 aggccagcga cgagtacagc gaactgctca cttcagcttg ctggcgatga gattggcgaa
     1801 gtcgccgacg ttcttcagct tggtcacttc ggccgcggag aacttgacgc ggaaggcttt
     1861 ctccaccgac agcaccacgc ggatatgcgc caggctgtcc caacccttga cctgcttggc
     1921 ggtgagctcg ggcgtggccg tgatcgcgtc gttgtcgaac acgtcgcgca ggacatcggt
     1981 gagcttggcg taaatgtcgg cgttttccac ggcttttcct gggcagtcga aggcgggcaa
     2041 gcttaggcga ggcctccgcg gcgggcaatc ggagaaaacc tccagtgcgc agggcgcagg
     2101 cggggcaatt ccgccctgct caacgcgggg taattccaat atcatcggcg ctgctacgca
     2161 gcccgagaat ttccgtgacg cccgacgcca tcgcccagcg ctacctagac ctgctcgccc
     2221 gtaccctcac cggcacgctg tacgaggagg agcccaacca cgataccgcc aaccagggcg
     2281 aattcgtggt cgcgttcacg ctgcattacc tgcgcggcag cgcggtgtcg atgcagccca
     2341 aggtgcggct ggacaacatc cgcgactgcg tcaagcaggt gctggccgac aacgtggccg
     2401 gcgatttcat ggaaaccggc gtgtggcgcg gcggcggctg catctacgcg cgcgcggcgc
     2461 tggacgtgta cggcggcggc gagcgcacgg tgtgggtgtg cgattcgttc gagggcatgc
     2521 ccaagcccga cgcggagaag catcccggcg aagccaagtt cttcgactcc ccgatgatgc
     2581 aggacatgta caaccggctg gaggcgacgc tgcccgacgt gacgcgcaac ttcgaggcgt
     2641 tccagctgtt gtcggacaag gtcaagttcg tcaagggctg gttcaaggac accatgccga
     2701 cggtgccggt gcagcagctg gccatcctgc gcctggacgg cgactactac gactcgacca
     2761 tggacgtgct caagcacatg tacggcaagg tcagcccggg cggctatgtc atcgtcgatg
     2821 actacggcga gaccggctgg gccaactgtc gcggtgccac cgacgatttc cgccgcgacc
     2881 agaacatcag cgcgccgctg atcaaggtcg attcgaaatg ctggtactgg cgcaagccgt
     2941 tctagacctc gcctagcgct gcgccgcggc gctcacttcc gcgtacagcg cgcgcgcgcg
     3001 ttccaccagc ggctggttgg cagcggagaa atattcgtcg cgcagccgct gcttgatgtt
     3061 gtcgcgctgc gccgatggcg acaggtgccc ggtgttgctg cggaacaccc gatcctggct
     3121 ctcggccgtg tgctggtagc ggtcgaactg cgtgccgaag cgcgcgttga gccggtcgat
     3181 caccgcgccg aagtcggcaa tggtctgctc gaagctggcc accacgcagc ggtccagcac
     3241 cggccgcacg cgctcatgga agcggatgta ctggcgtaaa taaacctcga ggctgacgat
     3301 ctgctccatt gccggcgaca cctggatgtt gagcgccttg agcgagggga tgcactggtc
     3361 gggatcgcgc accagcagca cggtgggtat gcccagttcc actgccttga ggatctgcgc
     3421 gtgcgaatgc acgtggtggc cgacgcgcac cggcgtgcgc tgtgcgccgc tgaaggcgcg
     3481 caccgagaag ctgttggacg agcgcggaaa gccgtcgatc accacctgcg tgttgcggtc
     3541 gacgatgttg tcgcggaagg tcgagtggcg gaagcgccag cgctgcagcc cgaggaagaa
     3601 atcggtgtgg tcttccagca tcaggcggaa cagcacccgg ccgcgccaca gcggcgccgc
     3661 gacggcgggg ttctgggtca atgcgttggg catcgggcgg gatcggcggc aaaaaatata
     3721 acatgcagtt cttccaaccc ggccggagcg cgcgatgact cgagggggcc gtctctatct
     3781 ctgggtgctg gccgccatcg tgctcggcgg catctgcggc gcgatcgatc cggccggcgc
     3841 ggtgaagctc aagccgctgt ccgacggctt catcgcgctg atcaagatgc tgatcgggcc
     3901 ggtgatcttc ctcaccgtgg tgctgggcat cgccggcatc gacagcgtga agaaggtggg
     3961 acgcatcggc ctcaaggccg tggtctattt cgaactgctg tccaccgtcg ctctggcgct
     4021 ggggctggtg atcgttaacg cactgcgccc cggcgccggc ttcaacgccg atgcggccac
     4081 gctcgatgcc ggcgcagtgg ccgagtactc gaagaaggcg gccgagcaga gcaccgcgga
     4141 tttcctgctg cacatcatcc cgaaaacctt cagcgacgcg ttttccggcg gcggcgacct
     4201 gctgcaggtg ctgctggtgg cggtgctgtt cgcctttgcg ctgtcggtca tcggcgagca
     4261 gggccggccg gtgatcgcgc tgctggaagg gctgtcgaaa gtgttcttcg gcatggtggc
     4321 gctggtcatg cggctggcgc ccgtcggcgc gggcgcggcg atggccttca ccatcggcaa
     4381 gtacggcgtg gccgcgctgg cgccgctggc gaagctcatg gccggctttt acgcgacgtg
     4441 cctgatcttc atcttcgccg tgctcggcat ggtctgccgg ctgtgcggat tctccatcgt
     4501 caagttcatc cgctacatcg gcgccgagtt gctgctggtg ctgggcactt catccagcga
     4561 aagcgcgctg gtgccgctga tggaaaagct cgagcgcgcc ggctgcgcgc gcagcgtggt
     4621 cggcctggtg atacccgccg gctattcgtt caacctcgac ggcaccaata tctacctcac
     4681 catggcggcg atcttcgtcg cgcaggcgct gaacatcgag ctgacgctgg cgcagcagct
     4741 gtccttgctg ctggtcgcca tgctcacctc caagggcgcg gccggcgtga ccggctcggg
     4801 cttcatcacg ctggcggcga cgctcgcggt ggtgccgtcg gtgccggtgg cgggcctcgc
     4861 gctgatcctg ggcatcgacc gcttcatgag cgaggcgcgc gcgctgacca acatcatcgg
     4921 caacggcgtg gcgaccatcg tggtggcgaa gtgggaaggc gaactggatg cgcgcaagct
     4981 ggccgcggag ctgcaaccgg ccgccaccgc accctcatcc agcctgttat aaaatattta
     5041 ttaaaagtta aataatatta tgttttataa cgtttcacgg gccggtgcgg tgggcatgaa
     5101 aaccgcgcac cgcggctagc gcagcagcac cttgagcatg gcatctacat gcgcatcgat
     5161 caccgcaggc tttgacgggt cgatcttcca cacgcgcttc acttccggcg ccagcgtgta
     5221 gaactgctgc gccgcgccga cgatgatgta gaccagcgcg gattcggaga cgtcgggcac
     5281 catgccttcc tgcttgagca ggcgcacgta gttgagcgcg gcgcgcgagg tgtgggtgac
     5341 gaaggtgtcg gccagccagc gcaggcgctt gctgtcgcgc acgctctcct ggaccatcag
     5401 ccgcgcgtgc tccgggtgct gcgcgctgta gcgcacgatg cgccgcagga tttcgcgcgc
     5461 atgctcgcgg cggccttcgg gcgtggccat ggcgccgggc ggtggatcga aagtgagatc
     5521 gccggcctgg cggtggaaca ggaaggtgac cgcggcgcgc cacagctgct ccttgctgtg
     5581 gtagtggtat ttgatcagcg cgtggtgcac gccggcgcgc gcggcgatgt cgctgttcat
     5641 cggatagaac tccgcggcga
//
LOCUS       DASZZC010000259         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_788329,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000259 DASZZC010000000
VERSION     DASZZC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788329"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..2846
                     /locus_tag="VHE37_05520"
     CDS             57..2846
                     /locus_tag="VHE37_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT35018.1"
                     /translation="MKRAAVIALAALPLAAAAASTVGELTPSGARQSGGHVLVQTPTI
                     LRLAPSAPVAADAHQAIEHYDEVIRWSDDPAIRAEALRRAAWLRLAEAEASGDTAASS
                     ELHAAVELYRRALADYPGDAANDRALYQLAHAYQLLGEPQQASDALQALTRAHPQSLL
                     AMDAHFRAGELLYLQQRYREAGAEYAVVVADGRARYRLPSLYKYAWSLYRQRQYEDAL
                     APFLELCAAYLPAGEFSAELRNVPAAELDLAQDALRMSALSLATLGPDGMARYFAARG
                     NPPYGAALYLATAAALERMQRYTDAADTYAAYAAAHVDDPRGPDFQDHAIVALLHGGF
                     GAQAVTAKTAYVERYAPDGAAWKQRALPPPVAAQLRRYAGELALYHRALGQQAQQRAE
                     FDQAASLYARLLRWFPDDPSAAAVNFSYAETLLDEHDEADAAREYAKTAYGYPRFERS
                     EDAAYAAVQVYRRLGDSAPAAARDTALRQSVAAGLQLASTFAQHPHRTAVMAQLAGDE
                     LELRDYDKAAATAALLLDGPQPLAAGTRADLLGVAADAQFALGRYPDSETQYTRLLAA
                     LDRGDARAAPARERLAESIYRQAEQARAAGDQRLAAAGFQRVGATVPDASIRASADYD
                     AAAALIQLQDWPAASGVLEGFVLRNPKHALLAQADRKLAWAYQNAQRPAPAAAVYQRI
                     AQRDSEPADVRMDAAWQAAALYRQAHQYADSAAAYRYYLASFAPPPERRLQALRELAD
                     LSHEQLGDEAGYRRWLGELIAAAGAAARGSAAQLMAANAALELGRAQAADAQRLTLRD
                     PLERSLKSRLGATSTAVDTLTRAAGYGLVEVTPAASYAVGQAYSDFAGAMLTSERPAK
                     LSGDARQQYDLLLEEQADPFEQKAIHAHEGNLARIEQGVWDRWVGASVDALGRLAPAR
                     YGKHENNETSYDSLK"
     gene            2830..>3237
                     /locus_tag="VHE37_05525"
     CDS             2830..>3237
                     /locus_tag="VHE37_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT35019.1"
                     /translation="MTRSSKRLLAASLALMLAACAAAPMHTLPPPRNIAAPAAAPAAP
                     ADNSAAAEHRFRDALALMKDQQVDEAIAAFTALDHDFPAYSGAATNLGILYARRQQLA
                     RARGWFERAAAQNPRNVVALSWLGSTRRALGDFP"
BASE COUNT          481 a         1230 c         1140 g          386 t
ORIGIN      
        1 caggcgcacc tgctgctggc gcgcctgcac gaccgcgaca gcgaggtggc cacgcgatga
       61 aacgcgccgc ggtcatcgca ctggccgcgc tgccgctggc cgcggcggcc gccagcaccg
      121 tgggcgaact cacgccgtcg ggcgcacgcc agtccggggg ccatgtgctg gtgcagacgc
      181 ccaccatcct gcggctcgca ccctcggcgc cggtggcggc agatgcgcac caggccatcg
      241 aacactacga cgaggtgatc cggtggagcg acgacccggc catccgcgcc gaggcgctgc
      301 gccgcgccgc gtggctgcgc ctggccgaag cggaggccag cggcgacacc gcagccagca
      361 gcgaactgca tgcggccgtc gagctgtacc gccgtgcgct tgccgattat cccggcgatg
      421 ccgccaacga ccgcgcgctg taccagctgg cgcacgccta ccagctgctc ggcgagccgc
      481 agcaggccag cgacgcgctg caggcgctga cccgcgcgca tccgcagtcg ctgctggcga
      541 tggatgctca tttccgcgcg ggagaactgc tctacctgca gcagcgctac cgcgaggccg
      601 gcgcggagta cgcggtggtc gtcgccgacg ggcgcgcccg ctaccgcctg ccctcgctct
      661 acaagtacgc ctggtcgctg taccggcaac gccagtacga agatgcactc gcgcccttcc
      721 tcgaactctg cgcagcgtac ctgcccgccg gcgagttctc cgcggagctg cgcaatgtgc
      781 cggccgcgga actggacctg gcgcaggacg cgctgcgcat gagcgcactg tcgctggcca
      841 cgctcggccc agacggcatg gcgcgctact tcgccgcgcg tggcaatccg ccctacggcg
      901 ccgcgctgta cctggcgact gccgccgcac tcgaacgcat gcagcgctac accgatgctg
      961 ccgataccta tgcggcgtac gcggcggcac acgtggacga tccgcgcggg ccggacttcc
     1021 aggatcacgc catcgtggcg ctgctgcacg gcggcttcgg cgcgcaggcg gtgacggcca
     1081 agaccgcgta cgtggagcgc tatgcgcctg atggcgccgc gtggaaacag cgcgcgctgc
     1141 cgccgccggt cgcggcgcag ctgcgccgct acgccggcga actcgcgctg tatcaccgcg
     1201 cgctcggcca gcaggcgcag cagcgcgcgg aattcgacca ggccgcttcc ctctacgcgc
     1261 gcctgctgcg ctggtttccc gacgatcctt ccgccgccgc cgtcaacttc agctacgccg
     1321 agacactgct cgacgagcat gacgaggccg acgccgcgcg cgagtacgcg aaaaccgcct
     1381 acggctatcc gcgcttcgag cgcagcgaag acgcggccta tgcggcggtg caggtgtacc
     1441 ggcgcctggg cgacagcgca ccggcggccg cgcgcgacac ggccctgcgc cagtcggtgg
     1501 cggccgggct gcagctggcc tcgaccttcg cccaacaccc gcaccggacc gcggtgatgg
     1561 cgcaactcgc cggcgacgaa ctcgagctgc gcgactacga caaggctgcg gcgacggcgg
     1621 cactgctgct cgacggcccg cagccgctcg ccgccggcac gcgggccgac ctgctgggcg
     1681 tagcggccga tgcgcaattc gcgctgggcc gctacccgga cagcgagacg cagtacacgc
     1741 gcctgctcgc agcgctggac cgcggcgatg cgcgcgccgc acccgcgcgc gagcgcctgg
     1801 cggaatcgat ctaccggcag gccgagcagg cgcgcgcggc cggcgaccag cgcctggccg
     1861 cggccggctt ccagcgcgtg ggcgccaccg tgcccgatgc cagcatccgc gccagcgctg
     1921 actacgacgc ggccgcggcg ttgatccagc tgcaggactg gccggccgcc agcggcgtgc
     1981 tggaaggctt cgtgctacga aatccgaagc acgcgctgct ggcacaggcc gaccgtaagc
     2041 tggcctgggc ctaccagaac gcccagcggc cggcgccggc ggcggcggtg taccagcgca
     2101 tcgcgcagcg cgacagcgaa cccgcggacg tgcgcatgga cgcggcctgg caggcagccg
     2161 cgctctaccg gcaggcacac cagtacgccg acagcgcggc cgcgtatcgc tattacctcg
     2221 ccagcttcgc gccgccgccg gagcgccggc tgcaggcgct gcgcgaactc gccgacctgt
     2281 cgcacgaaca gctgggcgac gaagccgggt atcgccgctg gctgggcgaa ctcatcgcgg
     2341 cagcgggcgc cgcagcgcgc ggcagcgccg cgcagctgat ggcggccaat gccgcgctgg
     2401 aactcggccg cgcgcaggcc gccgatgcgc agcgcctgac gctgcgcgac ccgctggagc
     2461 gcagcctcaa gtcgcggctg ggcgcgacca gcacagcggt cgacacgctg acgcgcgccg
     2521 cgggctacgg cctggtggaa gtgacgcccg ccgccagcta cgcggtcggc caggcctaca
     2581 gcgatttcgc cggcgccatg ctcacctccg agcggccggc aaaactgtcg ggcgatgcgc
     2641 gccagcagta cgacctcctg ctggaggagc aggccgaccc cttcgagcag aaggcgatcc
     2701 acgcgcacga gggtaatctc gcgcgcatcg agcagggcgt gtgggaccgc tgggtcggcg
     2761 ccagcgtgga tgcgctcggc aggctggcac cggcgcgcta cggcaagcac gaaaacaacg
     2821 agacgtccta tgactcgctc aagtaagcgc ttgctggcgg cctcgctggc gctgatgctg
     2881 gccgcctgcg cggcagcgcc gatgcacacg ctgccgccgc cgcggaacat tgctgcgccc
     2941 gcggccgccc cggcagcgcc ggccgacaac agcgccgcgg ccgagcaccg gttccgcgac
     3001 gcgctcgcgc tgatgaagga ccagcaggtg gacgaagcca tcgctgcctt caccgcgctc
     3061 gaccacgact tcccggccta cagcggcgcc gccaccaacc tgggcatcct ctacgcgcgc
     3121 cgccagcagc tggcgcgggc gcgcggctgg ttcgagcgcg cggccgcgca gaatccgcgc
     3181 aacgtggtcg cattgagctg gctgggcagc acgcgccgcg cgctcggcga ttttccg
//
LOCUS       DASZZC010000260         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_922942,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000260 DASZZC010000000
VERSION     DASZZC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922942"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..518)
                     /gene="tpx"
                     /locus_tag="VHE37_05530"
     CDS             complement(18..518)
                     /gene="tpx"
                     /locus_tag="VHE37_05530"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HVT35020.1"
                     /translation="MATVTLRGAPVQTSGELPKIGGEAPGFRLANRDLKDVSLHEFVG
                     KKKVLNIFPSIDTPVCATSVKKFNERMRGRSDVALLMIAADLPFAFKRFCGAENIDNV
                     ITLSTFRSQAFLKDYGVLIETGPLAGLAARACVVLDQDNKVIHAELVPEIGQEPDYDA
                     ALKAVA"
     gene            complement(529..1890)
                     /gene="gcvPA"
                     /locus_tag="VHE37_05535"
     CDS             complement(529..1890)
                     /gene="gcvPA"
                     /locus_tag="VHE37_05535"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HVT35021.1"
                     /translation="MPFIPHTETDVREMLATIGAPSIDALFDEIPAELRSGKLEKIPE
                     GLPEMEIAQLMRARAAQDGGALNFIGAGAYEHHIPAAVWEITTRGEFYSAYTPYQAEA
                     SQGTLQLLYEFQTMMTALTGMEVSNASMYDGASALGEALLMTMRANKEHAGGMVLIPR
                     TVNPIYGEVVLATTQAQGVRHALVEYDPRAGVTLSSALKNYEGMDLTAMVIQQPNFFG
                     VMEDVDALTDWAHAHGALVVAVVNPTSLALLKPPSQWGSKGADIVCGEGQPLGVPLSS
                     GGPYFGFLNTNMQYVREMPGRIVGRTLDLDGKPGYTLTLQAREQHIRRAKAKSNICTN
                     QGLLVTAATIYMSLLGPDGLAKVASACMANTRKLVAAVSKIGGVSAAFSGANFHEVVL
                     KLPQPAAITLAKMAARGVLGGFDLGKHYPELGDAILVCVTETKTDTQIQRYAEVLRQA
                     LAA"
BASE COUNT          310 a          640 c          626 g          375 t
ORIGIN      
        1 ctcatgttgt tttttcctca ggcgaccgct ttcagcgcgg cgtcgtagtc cggttcctgg
       61 ccgatttccg gcaccagctc ggcgtggatc accttgttgt cctggtccag taccacgcag
      121 gcgcgcgccg ccagcccggc cagcggcccg gtttcgatca gcacgccgta atccttgagg
      181 aaggcctggc tgcgaaaagt ggacagggtg atcacgttgt cgatgttctc ggcgccgcag
      241 aagcgcttga aggcgaacgg caggtcggcg gcgatcatca gcaacgccac gtcactgcgc
      301 ccgcgcatgc gctcgttgaa tttcttcacc gaggtggcgc acaccggcgt gtcgatcgac
      361 gggaagatgt tcagcacctt cttcttgccg acgaactcgt gcagcgaaac atccttgaga
      421 tcgcggttgg ccaggcggaa gccgggcgcc tcgccgccga tcttcggcag ttcgccgctg
      481 gtctgcaccg gggcgccgcg cagggtaacg gtagccatga gctctccttt atgccgccag
      541 tgcctggcgc agcacttcgg cgtagcgctg gatctgcgtg tcggtcttgg tctcggtgac
      601 gcacaccagg atggcgtcgc ccagttccgg gtaatgcttg cccaggtcga agccgccgag
      661 cacgccgcgc gcggccatct tcgccagcgt gatggccgcc ggctgcggca gcttcaatac
      721 gacttcgtga aaattggcgc cgctgaacgc tgcactgacg ccgccgatct tgctcaccgc
      781 cgcgaccagc ttgcgcgtgt tggccatgca ggccgatgcc accttggcca gtccatccgg
      841 cccgagcagc gacatgtaga tggtcgccgc cgtcaccagc aaaccctggt tggtgcaaat
      901 gttggacttg gccttggcac ggcggatgtg ctgctcgcgc gcctgcagcg tcagcgtgta
      961 gcccggcttg ccatcaaggt cgagcgtgcg gccgacgatg cggcccggca tctcgcgcac
     1021 gtactgcatg ttggtgttga ggaagccgaa gtaaggcccg ccggacgaca gcggcacgcc
     1081 cagcggctgg ccttcaccgc agacgatgtc ggcgcccttg ctgccccact gcgacggcgg
     1141 cttgagcagc gccagcgagg tcgggttgac caccgccacc accagcgcgc catgtgcgtg
     1201 cgcccagtcg gtgagtgcgt cgacgtcttc catcacgccg aagaaattgg gctgctggat
     1261 caccatggcg gtgaggtcca tgccttcgta gttcttcagc gccgaactga gcgtgacgcc
     1321 ggccctggga tcgtattcga ccagtgcatg gcgcacgccc tgcgcctgcg tggtggccag
     1381 caccacctcg ccgtagatgg ggttgaccgt gcgcgggatc agcaccatgc cgccggcatg
     1441 ttccttgttg gcgcgcatgg tcatcagcag cgcctcgccc aaggccgaag cgccgtcgta
     1501 catcgaggcg ttcgacacct ccatgccggt gagcgccgtc atcatggtct ggaattcgta
     1561 cagcagctgc agcgtgccct ggctggcctc ggcctggtac ggcgtgtagg cgctgtagaa
     1621 ctcgccgcgc gtggtgattt cccacaccgc cgccggaatg tggtgttcat aggcgccggc
     1681 gccgatgaag ttcaacgcgc cgccatcctg cgcggcgcgc gcgcgcatca gctgcgcgat
     1741 ctccatctcc ggcagtcctt ccggaatctt ttccagcttg ccgctgcgca gctcggcggg
     1801 aatctcgtcg aacagcgcat cgatcgacgg cgcaccgatg gtggcgagca tctcgcgcac
     1861 atcggtttca gtgtggggaa tgaaaggcat tgcttgattc gatccagaaa ttatccgagt
     1921 tcagccagaa ccttctgata gcccgcggcg t
//
LOCUS       DASZZC010000261         7800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1178268,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000261 DASZZC010000000
VERSION     DASZZC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178268"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VHE37_05540"
     CDS             <1..834
                     /locus_tag="VHE37_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HVT35022.1"
                     /translation="EQLDSAPGSVSQLRECAAQMVRHAKTSQTTVLLVGHVTKEGAIA
                     GPRVLEHMVDTVLYFEHDVGSRFRIVRAVKNRFGAVNELGVFAMTDSGLKEVTNPSAL
                     FLSRHEQPVPGSVVTVTRQGSRPLLVEVQALVDRSLSGTPRRVTLGLESNRLAMLLAV
                     LHRHGGVTLGDQDVFANVVGGMKIQETAADLPLVIAALSSYQGRPLPMDTVIFGEVGL
                     AGEIRPVQSGEERLQEAAKQGFKRALIPEANRPRKGAKLDIEVIPVARLDQALNFLRD
                     H"
     gene            848..1525
                     /locus_tag="VHE37_05545"
     CDS             848..1525
                     /locus_tag="VHE37_05545"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HVT35023.1"
                     /translation="MSSLIAAALFFVGIHVFISGTALRGFLVRRLGEKIYLAFFSALS
                     AGGLAWLIWAFTSVRVVVPTGLAGWRWFAVVLNFIALVLVSFGVLSRSPTAVGGARLL
                     DEDGEPARGMHRVTRHPMLWGFAIWAAVHMVFNPQSPALIFFGTFLVLALTGPLLIDA
                     KRAAVEGERWKRYLSATSNVPFAAIFAGRNRMAWDELVAFPLLAAAVITELLVLNHAA
                     WFGVPAL"
     gene            complement(1526..2818)
                     /locus_tag="VHE37_05550"
     CDS             complement(1526..2818)
                     /locus_tag="VHE37_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HVT35024.1"
                     /translation="MNDLPLPALGGLLFLLILCCAFFSGSETGLMTLNRYRLKHRADK
                     GDRGARLAQNLLKRPDRLIGMILLGNTVTIELATIVANAIGVHIAGDLGVAAATLGLA
                     VLLLVFAEVMPKTLAALHPERVGIPASYVYWPLQWLIMPVVFVVNLFSNGVLRVFGVS
                     PEDAAQHSLSSEELRSVVAEAGAMIPQRHQKMLLSILDLEKATVEDIMVPRNEIAGLD
                     LAEPWDKVRERIVSSQHTRVPVFEGSIDNLKGVVHMRRVVRLAADNQLDTDSLRALAR
                     EPYFIPEGTPLNQQLLNFQNQKRRIGFVVDEYGDIQGLVTLEDILEEVVGEFTSDPAT
                     RIKNVYADADGSYRVSGFVTIRSLNKNLGWKLPTGGPKTVNGLVLEKLENIPEPGKRI
                     EMGGYEFEITETRANAVKTARIRPPRTPGTDSEKKAAA"
     gene            complement(2902..3687)
                     /gene="ccsA"
                     /locus_tag="VHE37_05555"
     CDS             complement(2902..3687)
                     /gene="ccsA"
                     /locus_tag="VHE37_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HVT35025.1"
                     /translation="MNLSLAILAASLYLFATLAAWRGPRMTAGTGILLAAPLALHAWL
                     LARIVLLPGAVSIGLDEAFSLFAWQSAFLLWFFSWREPLQILGLALYPMAAIAALVGE
                     RWSTGVAEPADWKLALHIMLSLFSAGLLTLAAVQAGALSIQDRLLHRHHQSRLIRALP
                     PLLNMERVLFQLIFMGFFLLSLTLISGALFVNNLKSQHLTHKTVLSFAAWFMFGTLLW
                     GRWRHGWRGRTAIRWALSGYGALILAYFGSKLVLEKILGTHWT"
     gene            3798..4532
                     /locus_tag="VHE37_05560"
     CDS             3798..4532
                     /locus_tag="VHE37_05560"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HVT35026.1"
                     /translation="MGPGLRTTALLIDDEILIDAGTGVGDLSLSQQRRIKQVFLTHSH
                     LDHVCGLAFMADNLFDLIDRPIEVHATPETLAIIREHLFNWKIWPDFAKLPDEEHPLL
                     HFNQVETGTMMDIGGYRTLMAYPVLHTVPAVGYVVQSPQSTFAFSGDTYPTESLWQSL
                     NGLSQLDKLMIEVAFTDEDAELARVSRHFTPRLLGEELKKLKHRPELFLTHHKPGCEQ
                     IIEKECRAALKGWEYHHLRRGDIIMS"
     gene            4557..5921
                     /gene="ffh"
                     /locus_tag="VHE37_05565"
     CDS             4557..5921
                     /gene="ffh"
                     /locus_tag="VHE37_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HVT35027.1"
                     /translation="MFENLTTRLSGVLDAIRGQGRLTEEQVNGAARELRMALLEADVA
                     LPVIKDFIDKVKARALGVEITQSLTPGQQFLKVVQEELIATLGGTAEPLNLRAQPPAV
                     ILMSGLQGSGKTTTTGKLALMLKEREKKNVIVVSCDVYRPAAIEQLKIVAGNVGVEWF
                     PSEPGQKPEDIARAALAHARKQYADVLIVDTAGRTTVDEPMMQEIAQLNAALTPVETL
                     FVVDSMTGQDAARTAKAFADVLPLTGVVLTKTDGDARGGAALSVRHVTGKPIKFIGVG
                     EKSDKLEVFYPDRIANRILGQGDVLGLIEETARKVDQDKALKFAEKLKKTKSFDLSDL
                     REQMLQMQNMGSFESILEKLPGGLQMQQQMKNIDAGKQIRRSVAIINSMTPQERRFPD
                     LIKASRKRRIAAGSGLEVQEVNKLLRQFETTRDMMKKMAGGGMAKLMRGLAGRLPPGM
                     MPRR"
     gene            5930..6181
                     /locus_tag="VHE37_05570"
     CDS             5930..6181
                     /locus_tag="VHE37_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35028.1"
                     /translation="MDQDRWQQAQFCSDHLSAEVIAAWLRDAGIPVRIVDAGALPGLE
                     FGSRVLVPVELLHRASWMLKQLPVSDSELDALARSGQPE"
     gene            6402..7094
                     /gene="phoB"
                     /locus_tag="VHE37_05575"
     CDS             6402..7094
                     /gene="phoB"
                     /locus_tag="VHE37_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HVT35029.1"
                     /translation="MLDKTILVVEDEAAIREMIRFALARADFRVAQAANAQEARLAIA
                     ERVPDLILMDWMMPGTSGVELTRELKAAATTKDIPVIMVTARVEEEDKVRALNLGCDD
                     YVSKPFSFPELIARIQAVLRRTLPGGAEEKLAVNGLEVDAASQRVTAKGEPVRLGPTE
                     YRLLHFFVSHPERVYTREQVLDRVWGQNVYVEERTVDVHIRRLRKALEPHGYENMIQT
                     VRGTGYRFSEKP"
     gene            7145..>7800
                     /locus_tag="VHE37_05580"
     CDS             7145..>7800
                     /locus_tag="VHE37_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HVT35030.1"
                     /translation="MLLLPALRDEFPRIAAYALGGALAGWPLDSAALGACAGLALVLL
                     SYLRNLAALRAWAFAPKHHELPERGGIWGEVFYRLSDWQRRNKRKKKKLSNMLDEFRA
                     STAALPDGAVVLGESGEIMWYNRAAQTLLGLRPEDVGLRIANLVRHPAFSEYFGDGDF
                     AAREADERELEVPSPVAATRTLWLRIIPYGTNQRLLIVRDVSDIKRLEAARRDFVSNA
                     S"
BASE COUNT         1440 a         2392 c         2554 g         1414 t
ORIGIN      
        1 gagcagctcg attccgcgcc gggttcggtg tcgcaattgc gcgagtgtgc ggcgcaaatg
       61 gtgcgtcatg ccaagaccag ccagaccacc gtgctgctgg tggggcacgt cacgaaggaa
      121 ggcgccatcg ccgggccgcg cgtgctcgaa cacatggtcg acaccgtgct gtactttgaa
      181 cacgacgtcg gctcccgctt ccgcatcgtg cgcgcggtga agaaccgttt cggtgcggtc
      241 aacgaactcg gcgtgtttgc catgaccgac agcgggctca aggaagtgac caatccatcc
      301 gcgttgtttc tttcgcggca tgagcagccg gtcccgggaa gcgtcgtgac ggtcacgcga
      361 cagggcagcc gcccattgct ggtcgaggtg caggcgctgg tggatcgttc cctcagcggc
      421 acaccgcgcc gggttacgct cgggctcgag tccaaccgcc tggccatgct gctggcggtg
      481 ctgcatcgtc atggcggggt gaccttgggt gaccaggatg ttttcgccaa cgtggtgggc
      541 ggcatgaaga tccaggaaac cgctgccgat cttccgctgg tcattgccgc gctatcgagc
      601 taccaggggc ggccgctgcc gatggatacg gtgatctttg gcgaagttgg gctggcggga
      661 gagatccggc cggtccaatc gggagaggaa cgcctgcagg aagcggccaa gcagggattc
      721 aagcgtgcgc tgatcccgga agccaaccgc ccgcgcaagg gcgcgaagct cgacatcgag
      781 gtgatcccgg ttgcgcggct ggaccaggcg ctgaactttt tgcgtgacca ctgatccggg
      841 aaatgccatg tccagcctga tcgctgctgc gctgtttttt gtgggcatcc atgtcttcat
      901 ctcgggaacg gcgttgcgcg gattcctggt gcggcgcctg ggcgagaaaa tctacctcgc
      961 gtttttctcc gcgctttcgg cgggcggcct ggcctggctg atctgggctt tcaccagcgt
     1021 gagagtcgtg gtgcccacag gattggccgg ctggcgctgg ttcgcggtcg tgcttaattt
     1081 cattgcactg gtattggtgt ctttcggcgt gctgtcacgc agccccacgg cggtcggtgg
     1141 cgcgcggctg cttgatgaag atggcgagcc cgcgcgcggc atgcatcgtg tcacgcgcca
     1201 tcccatgtta tggggctttg ccatctgggc cgcggtgcac atggtgttca atccgcaatc
     1261 cccggcgctg atatttttcg gaaccttcct ggtgctcgcg ttgaccgggc cgttgttgat
     1321 cgacgccaag cgcgcggcgg tggagggcga gcgctggaaa cgctatttgt ccgcaacctc
     1381 caatgttccg tttgctgcca tcttcgcggg ccgcaaccgg atggcgtggg atgaattggt
     1441 ggcgttcccg ctgctcgcgg cggcagtgat caccgaactg ctggtactca accacgcggc
     1501 ctggttcggc gtgcctgcgc tctgatcagg ccgcggcttt cttttcactg tcggtgcccg
     1561 gtgtgcgcgg ggggcggatg cgcgccgttt tcactgcgtt ggcacgggtc tcggtgatct
     1621 cgaattcata gccgcccatc tcgatgcgct tgcccggttc cgggatgttc tcgagttttt
     1681 cgaggaccag cccattgacc gttttcggcc cgcctgtcgg caatttccag ccgagattct
     1741 tgttcaggct gcggatggtg acgaagccgc tgacccggta gctgccgtca gcgtcggcat
     1801 acacgttctt gatccgtgtc gccgggtccg aagtgaattc gccgactacc tcttcgagga
     1861 tatcctccag cgtcaccagg ccctggatgt cgccgtactc gtcgaccacg aatccgatgc
     1921 gccgtttctg gttctggaaa ttcagcagct gctggttgag cggcgtgcct tcgggaatga
     1981 aatagggttc acgtgccagc gcgcgcaggc tgtcggtatc cagctggttg tctgccgcca
     2041 gacgcaccac gcgccgcatg tgtaccacgc ccttgagatt gtcgattgat ccttcgaaca
     2101 ccggcacgcg ggtgtgctgg ctgctgacga tgcgctcgcg caccttgtcc caaggttcgg
     2161 ccaggtcgag gccggcgatc tcgttgcgcg gcaccatgat gtcttcgacc gtggctttct
     2221 ccaggtcgag gatggacagc agcatcttct ggtgacgctg tggaatcatg gcgcccgcct
     2281 cggcgacgac gctgcgcagt tcctcgctcg acaggctgtg ctgggccgcg tcttccggcg
     2341 acacgccgaa cacgcgcagc acgccgttgg agaacaggtt caccacgaac accaccggca
     2401 tgatcagcca ttgcaacggc cagtagacgt acgacgccgg aatgcccacg cgttccggat
     2461 gcagcgcagc cagggttttc ggcatcacct cggcaaaaac cagcagcagc acggccaggc
     2521 cgagcgttgc cgcggcgacg ccgagatcgc cggcaatgtg gacgccgata gcattggcga
     2581 cgatggtcgc cagttcgatg gtcacggtgt tgcccagcag gatcatgccg atcagccggt
     2641 cagggcgttt caacaggttc tgcgccagcc tggcgccccg gtcgcccttg tctgcgcgat
     2701 gtttcaggcg ataacggttc aacgtcatca gcccggtttc ggagcctgag aaaaacgcgc
     2761 agcagaggat cagcaggaac agcagtccgc cgagggcggg taggggaagg tcgttcaagt
     2821 gcgcggtcga gtggccgtga cttatgaata actacggaat cgaagccgcc gcgtcaactg
     2881 tgtttacatt aagaatatcg gctaagtcca atgcgtgccg agtatttttt cgagaaccag
     2941 cttgctgcca aaatacgcca ggatgagcgc tccgtagccc gacaaggccc agcggatcgc
     3001 tgtgcggcca cgccagccgt gccgccagcg gccccagagc agggtaccga acatgaacca
     3061 ggccgcgaac gagagcaccg tcttgtgcgt caggtgctgg ctcttcaggt tgttgacgaa
     3121 caaggcgccg gaaatgagcg tcagcgacag caggaagaag cccatgaaga tcagttgaaa
     3181 cagcactcgc tccatgttga gcaggggcgg aagcgcccgg atcagccgcg actgatgatg
     3241 ccggtgcagc aggcggtcct ggatcgagag cgcaccggcc tgcaccgcgg ccagcgtaag
     3301 cagtccggcc gagaacagcg acagcatgat gtgcagcgcg agtttccagt cggccggctc
     3361 cgccacgccg gtggaccagc gttcgcccac cagtgcggcg atggccgcca tcgggtagag
     3421 cgccagcccc aggatctgca gcggctcgcg ccaggaaaag aaccacagca ggaacgcgga
     3481 ttgccaggcg aacaggctga atgcctcgtc gagaccgatg ctgaccgctc cagggagcag
     3541 cacgatgcgg gcgagcagcc atgcgtgcag cgcgagcggc gcggcgagca ggatgccggt
     3601 acctgcggtc atgcgcggtc cacgccaggc ggcgagggtg gcaaaaaggt agagcgaggc
     3661 tgccaggatg gccagggaca gattcatcgg agtgagaata gcatcggcca gtggccgtac
     3721 gtccctgtgc taacatcgaa gcgcaacgat tcgtgcgaaa ttcaatgcga attaaagtgt
     3781 tgggctgcag cggaggagtg gggcccggcc tgcgcaccac tgccctgctg atcgacgacg
     3841 aaatactgat tgatgccggt accggcgtcg gtgatctgtc gctttcgcaa cagcggcgga
     3901 tcaagcaggt gtttctcact cactcgcatc tggatcatgt gtgcggcctg gccttcatgg
     3961 ccgacaacct gttcgacctg atcgaccgtc cgatcgaagt tcatgccacg ccggaaacgc
     4021 tggcgatcat ccgtgagcac ctgttcaact ggaagatctg gcctgacttc gccaagctgc
     4081 ctgacgaaga acatcctctg ctgcacttca accaggtgga aacgggcacg atgatggaca
     4141 tcggcggcta tcgcacgctg atggcttacc cggtgctgca cacggtgcct gcggtcggtt
     4201 acgtggtgca gagtccacaa tccaccttcg ccttctccgg cgatacctat cccaccgaga
     4261 gcctgtggca atcgctcaat ggcctgtcgc aactcgacaa gctgatgatc gaggtcgcct
     4321 tcaccgacga ggatgcggaa ctcgcgcgcg tttcgcgcca cttcactccg cggctgctcg
     4381 gcgaggaact gaaaaaactc aagcatcgcc ccgagttgtt tctcacgcat cacaagccgg
     4441 gatgcgagca gatcatcgag aaggaatgcc gcgccgcgct caagggctgg gaatatcatc
     4501 acctgcgccg cggcgacatc atcatgtcgt gacgcgctac acgctgagcc atatccatgt
     4561 ttgaaaatct gacgacgcgc ctgtccggcg tgctcgatgc catccgcggc cagggccgcc
     4621 tgaccgaaga gcaggtcaac ggtgcggcgc gcgagctgcg catggcgctg ctcgaagccg
     4681 acgtggcgtt gccggtgatc aaggatttca tcgacaaggt caaggcgcgc gcgctgggtg
     4741 tcgagatcac gcagtcgctg acgcccggcc agcagttcct caaggtggtg caggaggaac
     4801 tgattgcaac gctgggcgga actgcagagc cgctcaacct gcgcgcgcag ccgccggcgg
     4861 tgatcctgat gtccggcctg cagggttccg gcaagaccac caccaccggc aagcttgcgc
     4921 tgatgctgaa ggagcgcgag aagaaaaacg tcatcgtggt gtcgtgcgac gtctaccgtc
     4981 cggcggccat cgaacagctg aagattgtcg ccggcaatgt cggcgtcgaa tggtttccct
     5041 cggaaccggg gcagaagccc gaggacatcg cacgcgcagc cctggcccac gcgcgcaagc
     5101 aatacgccga cgtgctgatc gtcgacaccg ccggccgcac cacggtcgac gagccgatga
     5161 tgcaggagat cgcgcagctg aacgccgcgc tgacgccggt ggaaaccctg ttcgtggtcg
     5221 acagcatgac cggccaggat gcggcgcgta cggcgaaggc gtttgccgac gtgctgccgc
     5281 tcaccggcgt ggtgctgacc aagaccgacg gcgacgcgcg cggcggcgcg gcgctttcgg
     5341 tgcgccacgt caccggtaag ccgatcaagt tcatcggcgt gggcgagaag tccgacaagc
     5401 tcgaggtgtt ttacccggac cgtatcgcca accgcattct cggccagggc gacgtactcg
     5461 ggctgatcga ggaaacggcg cgcaaggttg accaggacaa ggcgctgaag ttcgctgaga
     5521 agctgaagaa gaccaagtcg ttcgacctgt cggacctgcg cgaacagatg ctgcagatgc
     5581 agaacatggg cagcttcgag tcgatcctgg aaaagctgcc gggcggcctg cagatgcagc
     5641 agcagatgaa gaacatcgac gcgggcaagc agatccgccg cagcgtggcc atcatcaatt
     5701 cgatgacgcc gcaggaacgc cgctttcccg acctgatcaa ggccagccgc aagcgccgca
     5761 tcgcggccgg ctccggtctg gaagtgcagg aagtgaacaa gctgctgcgc cagttcgaaa
     5821 ccacgcgcga catgatgaag aagatggcgg gcggcggcat ggccaagctg atgcgcggac
     5881 tggccggccg gctgccgccc ggcatgatgc cgcggcgctg aggctgccga tggatcagga
     5941 tcgctggcag caagcgcaat tctgctccga ccatctttcg gccgaagtga ttgccgcgtg
     6001 gttgcgcgac gccggcatac ctgtgcgcat cgtcgatgcc ggcgcgctgc ccggcctgga
     6061 gttcggctcg cgcgtgctgg tccccgttga actgctgcac cgagcgtcgt ggatgctgaa
     6121 gcaactgccg gtgagcgaca gcgaactcga cgccttggcg cggtccgggc agccggagtg
     6181 aggcgaaatc agcaaccgca ccggccctcg gggtgttata taagattgta taatattgaa
     6241 ataatcatat ttattataac aagctatacc ggcgtgcggt gctccatgac agagggattt
     6301 tttgattcat tgcggtgact tgcgccacat ttgtcacatg cttgcaaagt gacggcctta
     6361 cgctgccgcc atgagaatcc gtcgtatatt ggaacgcagt catgctggat aagaccatcc
     6421 tggtggtgga ggacgaggcg gcgatccgcg agatgatccg ctttgcgctc gcgcgcgcgg
     6481 atttccgcgt cgcccaggcc gccaacgccc aggaagcccg gctggccatt gccgagcgcg
     6541 tgccggacct gatcctgatg gattggatga tgccgggtac gtccggggtg gaactgacgc
     6601 gcgaacttaa ggcggcggca accaccaagg acatcccggt catcatggtg accgcgcggg
     6661 tcgaggaaga agacaaggtc cgcgcgctca acctcggatg tgacgactac gtttcaaaac
     6721 cgttttcctt cccggagctg attgcgcgca tccaggcggt gctgcgcaga accctgccgg
     6781 gcggagcaga agaaaaactc gcggtgaacg ggctcgaggt cgatgccgcc agccagcgcg
     6841 tcacggccaa gggtgagccg gttcggctcg gccccaccga ataccggctg ctccactttt
     6901 ttgtcagcca ccccgagcgc gtgtacacgc gcgagcaggt gctcgatcgg gtatggggcc
     6961 agaacgtcta cgtcgaggaa cgcacggttg acgtgcacat ccgccgcctg cgcaaggcgc
     7021 tcgagccgca cggctacgag aacatgatcc agacggtacg cggcaccggc taccgcttct
     7081 cggagaaacc ctgagcgaaa cgcccgacag cgccgccggg accggcttgt ccgcccggcc
     7141 gaagatgctg ctgctgcccg ccctgcgcga cgagttcccc aggatcgccg cctatgcgct
     7201 gggcggagcc ctggcgggct ggccgctgga ttcggccgcg ctcggcgcct gtgccggcct
     7261 ggcgctggtg ctgctcagtt acctgcgcaa cctcgcggca ctgcgtgcat gggccttcgc
     7321 ccccaagcac cacgagctgc cggagcgcgg cggcatctgg ggcgaagtgt tttatcgcct
     7381 gagcgactgg cagcggcgca acaagcgcaa gaagaaaaag ctgtccaaca tgctcgacga
     7441 gttccgcgcc tctactgcgg ctctgccaga cggtgcggtg gtgctgggcg agagcggcga
     7501 gatcatgtgg tacaaccgcg ccgcgcagac actgctcggg ttgcgccccg aagacgtcgg
     7561 cctgcgcatc gccaacctcg tgcgccatcc ggccttcagc gagtatttcg gcgacggcga
     7621 ttttgccgcg cgcgaggctg acgagcgcga gctggaagtg ccgtcgccgg ttgccgctac
     7681 gcgcacgttg tggctgcgca tcatcccgta cggtaccaac cagcgtctgc tgatcgtgcg
     7741 cgacgtctcg gatatcaagc ggttggaagc ggcacgccgc gacttcgtct ccaatgcctc
//
LOCUS       DASZZC010000262         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_535331,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000262 DASZZC010000000
VERSION     DASZZC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535331"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VHE37_05585"
     CDS             <1..955
                     /locus_tag="VHE37_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HVT35031.1"
                     /translation="AVRADVTVSMLGRKPGLHTGAGPDHAGEVVLALLELPEEIYDGV
                     APAAHLLRDTDLSGWLPRRARNSHKGTYGTVLVVGGDHGMMGAALLAARAALRAGAGL
                     VSVATRPEHAAAMTAAQPELMCRGVNSGRELAPLLREASAVVVGPGLGQDTWGRGLLA
                     RTLDSSLPLVVDADGLNLLSGEPDRRDGWILTPHPGEAARLLGGMRSADVQDDRLSAV
                     RNLREKYGGVAVLKGAGTLVQGSQLMLCPYGNPGMAGGGMGDVLGGVIAALLAQGLTL
                     ETAANAGVLAHALAGDRAAYGGERGMLAEDLLAELRVAVNA"
     gene            955..1446
                     /gene="tsaE"
                     /locus_tag="VHE37_05590"
     CDS             955..1446
                     /gene="tsaE"
                     /locus_tag="VHE37_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HVT35032.1"
                     /translation="MLTLQLPDAAATETLGAAIAHALQTYPGAVIYLEGDLGAGKTTL
                     ARGFLHQLGVTGAVRSPTYTLLEHYTAGERRVLHMDLYRLSDPLELGNLGLADFPYGS
                     TYWLIEWPRRGGALLPPAVITVRLAQLGSRRLAVVEANQVFGRDFDQDVEEFLRQRTK
                     NQP"
     gene            1542..>2142
                     /locus_tag="VHE37_05595"
     CDS             1542..>2142
                     /locus_tag="VHE37_05595"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HVT35033.1"
                     /translation="MRPLFFMLMLLLTLPAQAAELKDMRLWEGPDSTRVVFDLSATAS
                     HNIFTLENPSRLVVDISGIDAQGARIARRLDPLGVVQKVRSAPRPDGALRVVLDLSGP
                     VAPKSFILEPGADYGYRLVIDLYQPKPAADAPAADAAVAAVTTPPADAVPAEPALAAA
                     SAPAPPTAPAVVSTVPDPAPAPAPVPMAAPVVAAVVAAAP"
BASE COUNT          302 a          739 c          755 g          346 t
ORIGIN      
        1 ggcggtgcgc gcggatgtca ccgtgagcat gctcgggcgc aagccgggcc tgcacaccgg
       61 cgccggaccg gaccacgcgg gtgaagtggt gctggcgctg ctcgaactgc ccgaggaaat
      121 ctacgatggc gtggcgccgg ccgcgcacct gctgcgcgac accgacctgt ccggctggct
      181 gccgcggcgc gcgcgaaatt cgcacaaggg tacttacggc accgtgctgg tggtcggcgg
      241 cgatcacggc atgatgggcg cggccctgct tgcggcgcgt gcggcgctgc gcgccggtgc
      301 aggactggtc agcgtggcca cgcgacccga gcacgctgcc gcgatgaccg ctgcgcagcc
      361 ggagctgatg tgccgcggcg tgaattccgg gcgcgaactg gcaccgctgc tgcgcgaggc
      421 cagcgcggtg gtcgtcgggc ccgggctggg ccaggacacg tggggccgcg gcctgctggc
      481 gcgcacgctc gacagctcgt tgcccctggt agtcgatgcc gacgggctca acctgctctc
      541 cggcgagccg gatcgccgcg atggctggat tctcacgccg caccccggcg aagcggcgcg
      601 cctgctaggc ggcatgcgca gtgccgacgt gcaggatgat cgcctgtcgg ccgtgcgcaa
      661 cctgcgcgaa aaatacggcg gcgtggccgt gctcaagggt gccggcacgc tggtgcaggg
      721 cagccagctg atgctgtgcc cctacggcaa tcccggcatg gccggtggcg gcatgggcga
      781 cgtgcttggc ggcgtcatcg ccgcgctgct cgcccagggc ctgacgctgg agaccgcggc
      841 caacgccggc gtgctggccc atgcgcttgc cggcgatcgc gccgcgtacg gcggcgagcg
      901 cggcatgctg gcggaggatc tgctggcgga actgcgcgtg gcggtcaacg cctgatgctg
      961 acgctgcagc tgcccgatgc cgccgccacg gaaactctag gcgcggccat cgcccacgcc
     1021 ttgcagacct atcccggcgc ggtgatttac ctcgagggcg acctgggcgc gggcaagacc
     1081 acgctggcgc gcggcttcct gcaccagctg ggcgtgaccg gcgcggtgcg cagcccgacg
     1141 tatacgctgc tcgagcacta caccgcgggc gagcgccgcg tgctgcacat ggacctctac
     1201 cggctcagcg atccactgga gctgggcaat ctcggcctgg ccgattttcc ctacggcagc
     1261 acctactggc tgatcgaatg gccgcgccgc ggcggcgcgc tcctgccgcc agcggtcatt
     1321 accgtgcggc tggcccagct gggctcgcgc cgcctggcag tggtcgaggc aaatcaggtg
     1381 tttggccgcg acttcgacca ggatgtcgag gaattcttgc ggcagcgcac caagaatcaa
     1441 ccttaactaa ttgccgcatt tatctggtta ttaaagaaaa taaaacttgc gttagacaaa
     1501 ttatcacgct aaggtctgcg cattgtccga taggagcggg catgcgtccg ttgttcttca
     1561 tgctgatgtt gctgctcacg ctgccggcac aggcggcgga gctcaaggac atgcgcctgt
     1621 gggaagggcc ggactccacg cgcgtggtgt tcgatctgtc cgccaccgcc tcgcacaaca
     1681 tcttcaccct ggaaaatccc agccggctgg tggtcgacat ctccggcatc gatgcgcagg
     1741 gagcgcgcat cgcgcgccgg ctcgatccgc tcggcgtggt gcagaaggtg cgctcggctc
     1801 cgcggccgga tggcgcactg cgcgtggtgc tcgacctgtc gggtccggtg gcgcccaaga
     1861 gcttcatcct cgagcctggc gccgactacg gctaccggct ggtgatcgac ctctaccagc
     1921 ccaagcctgc tgccgatgct ccggccgccg acgccgccgt ggcggctgtg accactcctc
     1981 cggcagatgc cgttcccgcc gaacctgcgc tggcggccgc cagcgcgccg gctccgccta
     2041 cggcacctgc cgtggtttcg acggttcccg atcccgcgcc ggcaccggcg cctgtgccca
     2101 tggctgcgcc cgtggtggcg gccgtcgttg cggctgcacc ga
//
LOCUS       DASZZC010000263         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_788896,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000263 DASZZC010000000
VERSION     DASZZC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788896"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /gene="purH"
                     /locus_tag="VHE37_05600"
     CDS             <1..939
                     /gene="purH"
                     /locus_tag="VHE37_05600"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HVT35034.1"
                     /translation="FEKLQDLRYGENPHQRAAFYRERKPEAGSIAAARQLQGKELSYN
                     NIADADAALECVKYFSHPACVIVKHANPCGVAVSDDGIGQAYELAYQTDPTSAFGGII
                     AFNRELDAATAKTIVDRQFVEVIIAPSVTVEARAVVGAKANVRLMESGQWPRPSAPAF
                     DYKRVGGGLLVQDRDDAVVRASELKIVSKRAPTETELRDMVFAWKVAKFVKSNAIVYA
                     RNRQTIGIGAGQMSRVYSARIAGIKADDVKLAVPDSVMASDAFFPFRDGIDAAAAAGI
                     SAVIQPGGSVRDHEVIAAADEHNMAMVFTGIRHFRH"
     gene            complement(1035..1895)
                     /locus_tag="VHE37_05605"
     CDS             complement(1035..1895)
                     /locus_tag="VHE37_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HVT35035.1"
                     /translation="MKIHLTIATIALLATGASWAQSSIDEHRPLNADARISVNNTAGS
                     INVQAWDKNELHLTGSLGSGVDKLELSGDASNLSIEVKLPHKSHSVDDTTLTLQVPAG
                     VKLSLQGVSADIRVQGVRGSVEVNSVSGDINLDVKSAQVSAQTVSGDVHVQAPATSTK
                     VNSVSGDVVVRGVSGALQGEAVSGSLDVDGGPMSDVHVNSISGDVDLSVSLADRAKVA
                     AETLSGDVKLRLPSAPSAQLTLKSFSGDVRSDYNAVPAQAKRYEAAMGAGGGSISLSS
                     FSGDVSITRR"
     gene            complement(1907..2353)
                     /locus_tag="VHE37_05610"
     CDS             complement(1907..2353)
                     /locus_tag="VHE37_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35036.1"
                     /translation="MSEDDDRVPGLDRLPLRRAPESDLWPGIQARLKPRRQFSAPPWL
                     AAAACAVLALAVMLRYGLHDDVPEAQSALPQVASTQLPQPRAPLHAQMRAEVRGLVRA
                     NLQVVDGADRQIRQAMNYEPDSPLLQRLLARNQEQRRGLRQLLDQT"
     gene            complement(2350..2928)
                     /locus_tag="VHE37_05615"
     CDS             complement(2350..2928)
                     /locus_tag="VHE37_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT35037.1"
                     /translation="MAGGGILELEAPRDEAMAEEAAQVHAAQRGDARAFEQLYRRHRD
                     RIYGLCWRLHDGDRAKAEQSTQDAFVRAWDKLGGFRGDAQFGTWLHALTVNLVLGEKR
                     LLKRWVTFEDSGEGEDMETALVEHPQASAGLKMDLERALARLPRGARTVLLLHDVEGY
                     QHGEIAAATGIAVGTSKAQLHRARKLMKEYLQ"
BASE COUNT          456 a         1091 c         1007 g          502 t
ORIGIN      
        1 ttcgagaagc tgcaggatct gcgctatggc gaaaacccgc accagcgcgc cgccttctac
       61 cgggagcgca agcccgaagc cggcagcatt gccgccgcgc gccagctgca gggcaaggaa
      121 ctgtcataca acaacatcgc cgacgccgac gccgcactgg aatgcgtgaa atatttcagc
      181 catcccgcct gcgtcatcgt caagcacgcc aatccctgcg gcgtggcggt gtcggacgac
      241 ggcatcggcc aggcctacga actggcctac cagaccgatc ccacctcggc cttcggcggc
      301 atcatcgcct tcaaccgcga gctggatgcg gcgaccgcga agaccatcgt cgaccgccag
      361 ttcgtcgagg tgatcatcgc gcccagcgtc accgtcgagg cgcgcgcagt ggtgggagcc
      421 aaggccaacg tgcggctgat ggaatccggc cagtggccac ggccatcggc gccggcgttc
      481 gattacaagc gcgtgggcgg cggcctgctg gtgcaggacc gcgacgacgc cgtggtgcgt
      541 gccagcgagc tgaagatcgt cagcaagcgg gcgcccaccg agaccgagct gcgcgacatg
      601 gtctttgcgt ggaaggtcgc caagttcgtc aagagcaacg cgatcgtcta tgcgcgcaac
      661 cgccagacga tcggcatcgg cgccggacag atgagccgcg tctactcggc gcgcatcgcc
      721 ggcatcaagg ccgacgacgt caagctggcg gtgccggatt cggtgatggc gtcggatgcg
      781 ttctttccgt tccgcgatgg tatcgacgcc gccgccgccg cgggcatttc cgcggtgatc
      841 cagcccggcg gctcggtgcg tgaccacgag gtgatagccg cggccgacga gcacaacatg
      901 gcgatggtgt tcaccggcat ccgccacttc cggcactgag ccctccctgg accaggaccg
      961 cgccttccct ggcgcggact tcaagggcgg cgcaacgcgc cggctatttt cgatgatgca
     1021 attcggccgt tgcgctatcg gcgcgtgatg ctgacgtcgc ccgagaagct cgacagcgag
     1081 atgctgccgc cgccggcacc catggcagct tcatagcgct tggcctgcgc cggcactgcg
     1141 ttgtagtcgc tgcgcacgtc gccggagaag ctcttcagcg tgagctgggc cgagggcgcc
     1201 gagggcaggc gcagcttgac gtcgcccgac agcgtctccg ccgccacctt ggcgcggtcg
     1261 gccagcgaca ccgacaggtc gacgtcgccg gaaatgctgt tgacgtgcac atcgctcatc
     1321 ggaccgccgt cgacgtcaag cgagcccgac accgcctccc cctgcaacgc gccggacacg
     1381 ccgcgcacca ccacgtcgcc cgacacgctg ttgaccttgg tcgacgtggc cggcgcctgc
     1441 acatgcacgt cgccgctcac cgtctgcgcg ctgacctgcg ccgacttcac gtcgaggttg
     1501 atgtcgcccg acacgctgtt cacttccacg ctgccgcgca cgccctgcac gcggatgtcg
     1561 gccgacacgc cctgcaggct gagcttcacg ccggccggca cctgcagcgt cagcgtggtg
     1621 tcgtccacgc tgtgcgactt gtgcggcagc ttgacttcga tgctcaggtt cgacgcgtcg
     1681 ccggagagtt ccagcttgtc cacgccgctg ccgagcgagc cggtcaggtg cagctcgttc
     1741 ttgtcccagg cctgcacgtt gatgctgccc gcggtgttgt tcaccgagat gcgcgcatca
     1801 gcgttcagcg ggcgatgctc gtcgatggat gactgcgccc agctggcgcc ggttgccagc
     1861 aacgcaatcg ttgcaatggt cagatgaatc ttcatggcgc tctccttcag gtctggtcga
     1921 gcagctggcg caggccgcgc cgctgctcct ggttgcgcgc cagcaggcgc tgcagtaacg
     1981 gggaatcggg ttcgtagttc atggcctggc ggatctgccg gtcggcgccg tccaccacct
     2041 gcaggttggc gcgcaccagg ccgcgcacct cggcgcgcat ctgcgcgtgc agcggcgcgc
     2101 gcggctgcgg cagctgcgtg ctggccacct gcggcagcgc agactgcgct tccggcacat
     2161 cgtcgtgcag gccgtagcgc agcatcaccg ccagcgccag caccgcgcag gcagccgccg
     2221 ccagccacgg cggcgcggaa aactgccggc gcggcttgag gcgcgcctgg atgccgggcc
     2281 acaggtcgct ctccggcgcg cggcgcagcg gcagccggtc gagcccgggc acgcgatcgt
     2341 cgtcttcact cattgcaggt actccttcat cagcttgcgc gcgcggtgca gctgcgcctt
     2401 cgacgtaccc accgcgatgc cggtcgccgc tgcgatctcg ccatgctggt agccctccac
     2461 gtcgtgcagc agcagcacgg tgcgcgcacc cctgggcagg cgcgccagcg cccgctccag
     2521 gtccatcttc aatccggcgc tggcctgtgg atgctcgacc agcgccgtct ccatgtcttc
     2581 gccctcgccg ctgtcctcga aggtgaccca gcgcttgagc aggcgcttct cgcccagcac
     2641 caggttcacc gtcagcgcgt gcagccaggt gccgaactgc gcgtcgccgc ggaagccgcc
     2701 gagcttgtcc caggcgcgga cgaaggcgtc ctgcgtcgac tgctcggcct tggcgcggtc
     2761 gccgtcatgc agccgccagc acaggccgta gatgcggtcg cggtggcgcc ggtacagctg
     2821 ctcgaacgcg cgcgcgtcgc cgcgctgggc ggcatgcacc tgcgccgcct cctcggccat
     2881 ggcctcgtcc cgcggcgcct ccagttccag gatgcctccc cctgccatgc tcgctgctgc
     2941 caccgtggtc atcgcgcctt ggatgcggca gcgtcgccag gggtttaaag cataatggcg
     3001 cgcgattttt tttggtgtcc tgagggggaa ccatgccaca tggacttgcg gtgacc
//
LOCUS       DASZZC010000264         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_339625,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000264 DASZZC010000000
VERSION     DASZZC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339625"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VHE37_05620"
     CDS             <1..1120
                     /locus_tag="VHE37_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT35038.1"
                     /translation="PGCALAVFGVCALLLAVSTYYSRGLVIGDSGRGAPELRKESRYN
                     YDNDVIGDNYNIGTDVLTVIVEAKKFSGDSCLHFPVVDLIDRLELFVKGIDGVRSVTS
                     VAGVGKLIIGAFNEGNPRWRALPRSSAGLSTGSKAFDPGLGLNNEGCRAIKVMVFTNN
                     HDGTTVAHVVEEIKKFIAANHVDGVELRLASGNVGVMAATNEAVSEAEIRMLLSIFGA
                     LTLLCLLTFRSVKATLCVLVPLTIVSIFCNAVMARLGIGLKVATLPVVALGVGVGVDY
                     GIYLFERIQHDMADGMAFRDAFYQAMRQRGTAAVFTAITMSIGVGTWTLSALKFQADM
                     GLLLSFMFLVNMLGAVCLLPALGAWFYGPGKRRADTAA"
     gene            complement(1145..1405)
                     /locus_tag="VHE37_05625"
     CDS             complement(1145..1405)
                     /locus_tag="VHE37_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35039.1"
                     /translation="MTVSKCQMCGSAELRLVTGFAPFEFNGRSCRYPTRSVLCGECDS
                     ITTLPEQQQFNAESLARARRFVATHAQTGQHPAFPAGQPVPG"
BASE COUNT          258 a          513 c          543 g          302 t
ORIGIN      
        1 accgggctgc gcgctggcgg tgttcggcgt ctgcgcgctg ctgctggcgg tgagcacgta
       61 ttactcgcgc gggctggtca tcggcgattc cggccgcggc gcgccggagc tgcgcaagga
      121 gtcgcgttac aactacgaca atgacgtcat cggcgacaac tacaacatcg gcaccgacgt
      181 gctgacggtg atcgtcgagg cgaagaaatt ctccggcgac tcctgcctgc atttcccggt
      241 ggtggacctg atcgaccgcc tcgaactgtt cgtcaagggc atcgacggcg tgcgctcggt
      301 gaccagcgtg gccggcgtgg gcaagctgat catcggcgcg ttcaacgagg gcaacccgcg
      361 ctggcgcgcg ctgccgcgct cgagcgcggg cctgtcgacc ggctccaagg cgttcgaccc
      421 gggcctgggc ctgaacaacg agggctgccg cgcgatcaag gtgatggtgt tcaccaataa
      481 ccacgatggc accaccgtgg cccacgtggt ggaggagatc aagaagttca tcgccgccaa
      541 ccatgtcgac ggcgtcgagc tgcggctggc ctcgggcaac gtcggcgtga tggcggcgac
      601 caacgaggcg gtgagcgaag ccgagatccg catgctgctg tcgatcttcg gcgcgctgac
      661 gctgctgtgc ctgctcacct tccgctcggt gaaggcgacg ctatgcgtgc tggtgccgct
      721 gaccatcgtg tcgatcttct gcaacgcggt gatggccagg ctgggcatcg gcctgaaagt
      781 ggccacgctg ccggtggtgg cgctgggtgt cggcgtcggc gtcgactacg gcatctacct
      841 gttcgaacgc attcaacacg acatggccga cggcatggcc ttccgcgacg cgttttatca
      901 agcgatgcgc cagcgcggca cggcagcggt gttcaccgcc atcaccatgt cgatcggtgt
      961 cggcacctgg acgctgtcgg cgctcaagtt ccaggctgat atgggcctgc tgctcagttt
     1021 catgttcctg gtgaacatgc tcggcgccgt gtgcctgctg cccgcgctgg gtgcgtggtt
     1081 ctacgggccg ggcaagcgcc gcgcggacac ggcggcgtaa agcggcgcgg ggatcagccg
     1141 gccatcagcc gggcaccggc tggccggccg ggaaagccgg gtgctggccg gtctgcgcat
     1201 gcgtggcgac aaagcgcctg gccctggcca gcgactccgc gttgaactgc tgttgttccg
     1261 gcagcgtggt gatgctgtcg cattcgccgc acagcaccga ccgcgtcggg tagcgacaac
     1321 tcctgccgtt gaactcgaac ggcgcgaacc cggttacaag cctgagttcc gccgagccgc
     1381 acatctgaca cttggacacc gtcatcagaa agcccccgct tcctggacct gctccttttt
     1441 cccccgcggt tgcttcttgc atgtccctat gccggcaacc tgatcggtag tcgaacttta
     1501 catgatgtat agatgtaggg cgagattttt tgcaggactg ctggtggcgc gacgggcgcg
     1561 ccctgcgcaa aaaaaacatt gcgtcgtgtc ctcgggagtt gaccgcaccc gcctgc
//
LOCUS       DASZZC010000265         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_788996,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000265 DASZZC010000000
VERSION     DASZZC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788996"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..545)
                     /locus_tag="VHE37_05630"
     CDS             complement(105..545)
                     /locus_tag="VHE37_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVT35040.1"
                     /translation="MPSTIRLHRVLRAAPEKIYRAFLDAEAKVKWLPPNGFTAKVHHL
                     DAKVGGSYRMSFTNFGNGQTHTFGGTYTELVPHERIRYTDRFDDANLPGEMQVTITLK
                     KVFCGTELSIVQEGVPDAIPAEGCYLGWQESLVLLGKLVEADIP"
     gene            836..2017
                     /locus_tag="VHE37_05635"
     CDS             836..2017
                     /locus_tag="VHE37_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HVT35041.1"
                     /translation="MGTSAYVAGVGMIPFTKPGASKSYAEMGAEAVRLALADAWISYE
                     EIQQAAVGYVYGDSACGQTALYGVGMTGIPVFNVNNNCSTGSSALFMARQWVESGAAE
                     CVLALGFEQMQPGALKSHWDDRPSPFARFDALCDEINPYPEIPLALRYFGGAGKEYMD
                     KYGTGPELFAQVRAKASRHAEHNPMSLLRKVVTTEDVLAAPLVFPQAKLTRLMACPPT
                     CGAAAAVIVSERFARAKTKAPVRIAAQAMTTDYPSTFGERSMMKLVGFDMARRAADRA
                     YEQAAIGPDDVDVIELHDCFTANEILTYEALGLCGEGEAEKLIEDGDNTYGGKWVTNP
                     SGGLLSKGHPLGATGLAQCTEIVTQLRGRAGVRQVENARIGLQHNLGLGGACVVTLYQ
                     V"
BASE COUNT          379 a          719 c          721 g          363 t
ORIGIN      
        1 gacgagcgca tctcgcgcgc attccgcgcc atcgcgcgcg acaacgccgg acgggtcgcc
       61 gcgctcacct ggtgatgcat gttcagcggg gcgccgcgtc gcgcctacgg gatatcggct
      121 tccaccagct tgcccagcag caccagcgat tcctgccaac ccaggtagca gccctcggcg
      181 ggaatcgcat cgggcacacc ttcctgcacg atgctgagct cggtgccgca gaaaactttc
      241 ttcagcgtga tggtcacctg catttcgccg ggcaggttgg cgtcatcgaa gcggtcggtg
      301 tagcggatgc gctcgtgcgg caccagttcc gtgtaggtgc cgccgaaggt gtgcgtctgc
      361 ccgttgccga agttggtgaa cgacatccgg tacgagccgc cgactttcgc atccaggtga
      421 tggaccttcg cggtgaaccc gttcggcggc agccatttca ccttggcctc ggcatcgaga
      481 aacgcgcggt agattttttc cggcgcggcg cgcaggacgc ggtgcaggcg gatggtgctg
      541 ggcatgggtt gtctccgggt cgttgatgat gtcttccata actggtcgaa cggccctgcg
      601 gcaaatcgac agccgctgcg cggcctggcc gcggcaaaaa atgagtccac cgcatcaccg
      661 gttccggtgt tatgaaatat tgatttaatg ttaaataatc agataaacaa taacagcgaa
      721 aatgccggtg cggtcaggtg gtttttgcaa tattccgcgc aacccacccg cccggggagg
      781 gccgcacggc cacgcgagcg gccacactct gcgccagacc ttcaggagtt ttgccatggg
      841 cacatccgct tacgttgccg gcgtcggcat gatcccgttc accaagcctg gcgcgagcaa
      901 gagctacgcc gagatgggcg ccgaggccgt gcgcctggcg ctggccgacg cctggatctc
      961 gtacgaagaa atccagcagg ccgcggtcgg ctatgtctac ggcgattccg cctgcggcca
     1021 gaccgcgctc tacggcgtgg gcatgaccgg catcccggtg ttcaacgtca acaacaattg
     1081 ctcgaccggc tcgtcggcgc tgttcatggc gcgccagtgg gtggagagcg gcgccgccga
     1141 gtgcgtgctg gcgctgggct tcgagcagat gcagccgggc gcgttgaaga gccactggga
     1201 tgatcgccct tcgccgttcg cgcgcttcga cgccttgtgc gacgagatca acccgtatcc
     1261 cgagattccg ctcgcgctgc gctacttcgg cggcgccggc aaggaataca tggacaagta
     1321 cggcaccggc cccgagttgt tcgcacaggt gcgcgccaag gccagccgcc acgccgagca
     1381 caaccccatg tcgctgctgc gcaaggtcgt caccactgaa gacgtgctgg ccgcgccgct
     1441 ggtgttcccg caggcgaaac tcacgcgcct gatggcctgc ccgccgacct gcggagcggc
     1501 ggcggcggtg atcgtttcgg aaagattcgc gcgcgcgaaa accaaggcgc cggtgcgcat
     1561 cgccgcgcag gccatgacca cggattaccc gagcaccttc ggcgagcgca gcatgatgaa
     1621 gctggtcggc ttcgacatgg cgcgccgcgc cgccgaccgc gcctacgagc aggcggcgat
     1681 cggcccggac gacgtcgacg tgatcgagct ccacgactgc ttcaccgcga acgagatcct
     1741 cacctacgag gcgctcggcc tctgcggcga gggcgaggcc gagaagctca tcgaggacgg
     1801 cgacaacacc tacggcggca agtgggtgac caatccgtcg ggcggcctgc tctccaaggg
     1861 ccatccgctc ggcgccacgg gcctggcgca gtgcaccgag atcgtcacgc agctgcgcgg
     1921 gcgcgccggc gtgcgccagg tcgagaacgc gcgtatcggc ctgcagcaca acctcgggct
     1981 gggcggcgcc tgcgtcgtca cgctgtacca ggtttgagga ggcgaccatg acttcgccct
     2041 ggatgacacc ggaactggac acgttccgcg atgccgtgca ccgcttcgtc gcgcatgaag
     2101 tcgtgccgca ccagcagcgc tggcgcgagc agcagcacgt cgaccgcgcg ctgtggaaca
     2161 aggccggcga actcggtctg ct
//
LOCUS       DASZZC010000266         9023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_472453,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000266 DASZZC010000000
VERSION     DASZZC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472453"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VHE37_05640"
     CDS             complement(<1..763)
                     /locus_tag="VHE37_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HVT35042.1"
                     /translation="MQNQNEDIWIQCVRWLEAELPDKDISTWIRPLHPVASRDKILLL
                     APNRIVLERVRNDFLATIRRAVKANGGKNPPEVELAVGAGEARVNGVQPATAEETPAQ
                     FVAKLDPRYTFANFIEGKSNSQARAAAQHVVETPGGAYNPLLIYGDSGLGKTHLMHAI
                     GNTILKAQPRARIAYLGAEQWLNHMVAAIRHGKQDEFKTFYRSVDALLIDDIHFFAHK
                     ERTQEEFFHTFNALIDSHKQIVLTCDRYPKDVDGID"
     gene            923..1057
                     /gene="rpmH"
                     /locus_tag="VHE37_05645"
     CDS             923..1057
                     /gene="rpmH"
                     /locus_tag="VHE37_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HVT35043.1"
                     /translation="MKRTFQPHNKRRKRTHGFRARMATVGGRATLARRRAKGRKRLIP
                     "
     gene            1074..1436
                     /gene="rnpA"
                     /locus_tag="VHE37_05650"
     CDS             1074..1436
                     /gene="rnpA"
                     /locus_tag="VHE37_05650"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HVT35044.1"
                     /translation="MARLLSRARLHRPEDFKAALEQGRRVGTGLLSAAVRHNGLGIPR
                     LGLALAKKNIALATMRNRIKRHIRESFREHQAALPSADIVVFAKASAARATQADLHAA
                     LEQLWQRVNQACATSPSF"
     gene            1412..1627
                     /gene="yidD"
                     /locus_tag="VHE37_05655"
     CDS             1412..1627
                     /gene="yidD"
                     /locus_tag="VHE37_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HVT35045.1"
                     /translation="MRHLALFLIRAYQLLVSPLLGPRCRFHPSCSHYAAEAIHRYGVL
                     RGSWLALRRIGRCSPLHPGGYDPLPEK"
     gene            1643..3301
                     /gene="yidC"
                     /locus_tag="VHE37_05660"
     CDS             1643..3301
                     /gene="yidC"
                     /locus_tag="VHE37_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HVT35046.1"
                     /translation="MENRRFILIALLGVVLFFIYQAWQTDYPPPAVAPAAAAASLPGE
                     SAPSPAPTSSSTTTLPAEASATDVPATPIQVHTDLVNAEISPNGGDLDRVELLGYPEF
                     KDQPENLALLDNRNGRWFVLQSGLASSQDALVTPQSLFMSAASSYQLAPGTDTLDVTL
                     EHAAPAGYRVRKTYRFHRGSYEIELIQTLVNGSDAEISASPWVRFARLPYTVGYQPKF
                     SKTFAGEGLYVQKEGSTSYRFDKISFDDLSKKPTTVQQTGGWVAMLQHYFVAAIIPPA
                     DEKLTLTAKPGKDRGFVGQYVGAFATAPVHGEHSYSTKLYIGPELQDRVADIAPGFEL
                     TLDYGILTPIAKPLFWVLQLYHRLLGNWGFAIILLTLTVKLAMLKLSEAQYRSMAKMK
                     KFGPRIQELKERYADDRERQQKAMMDLYKKEGFNPLAGCWPLLLQMPVFFALLWVLQQ
                     SVELRMVPFALWVKDLSAPDPYYVLPVLYGAAMWAQQKISGQNAAMDPMQQKMMNVMP
                     IALTAFFTFWPSGLVLYWLASTLIGIAQQAYITRKYAEEGAARA"
     gene            3321..4670
                     /gene="mnmE"
                     /locus_tag="VHE37_05665"
     CDS             3321..4670
                     /gene="mnmE"
                     /locus_tag="VHE37_05665"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HVT35047.1"
                     /translation="MQFDDTIAAIATAPGRAAVGVVRLSGPRALSIAQKICGDLPPPR
                     AARVRALRAANGAVIDRGLVLVFPAPHSYTGDDVVELQAHGGPVLLNLLLRSACEFGA
                     RLARPGEFSQRAFLNERLDLAQAEAVADLIDAASEQAARAAQRSLAGELSQHVHALTQ
                     QLIGLRTYVEGALDFSDEDVDWLGDTAWRERLDRVLRDLDQLLKLCAQGRRLREGMVV
                     ALSGQPNVGKSTLLNRLAGSDSAIVTDIAGTTRDVLREHIVIGGMPVTLVDTAGLRHS
                     DDPVEAEGIRRAWSAVADAELTLFMVDDRAGITAADRDLLARMGAGARVLIVRNKCDL
                     SARVAEIFEQDGWTQLRLSATTGDGVDLLRARIEYAAGLDAHGEGVFSARARHLDAMH
                     AARAHLQRAAQHIAAHSSAELAAEELRLAQQALNEITGEFTSDDLLGRIFGSFCIGK"
     gene            4706..5143
                     /locus_tag="VHE37_05670"
     CDS             4706..5143
                     /locus_tag="VHE37_05670"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HVT35048.1"
                     /translation="MDAAALTAYLHQHIPLTRALGARVESCGHGSAVVSAPLEPNLNH
                     RNTAFGGSLATLAILSGWVVLHVALREAGIDARLVVQKHECEFQQPVTAAFTARSTLP
                     PRDWEKFRAALQRRGRARISVVSELRSGDALVVQSTGTFVALT"
     gene            5140..5664
                     /locus_tag="VHE37_05675"
     CDS             5140..5664
                     /locus_tag="VHE37_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVT35049.1"
                     /translation="MKPYKFCPQCGAPLSSGQHGGFARMGCTAQGCGFVHWDNPVPVV
                     AAIVEHEGGIILARNKLWPMKFYGLITGFLEKNDPSPGEAAVREVKEELGLNGGAPTF
                     VGHYPFERMNQIIIAFHVPAAGEIVLGEELSEYKRIAFGEARYWPAGTGLALRDWLRT
                     RGYDPQPMEMPKPR"
     gene            5826..8498
                     /gene="aceE"
                     /locus_tag="VHE37_05680"
     CDS             5826..8498
                     /gene="aceE"
                     /locus_tag="VHE37_05680"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HVT35050.1"
                     /translation="MTAEPPASPADLDPQETREWLDALDAVLQRVGPERAHFLLETLS
                     EKARRTGAFIPFSANTAYLNSIPPHLEERSPGDHALEWKIRSIIRWNAMAMVVNANRE
                     HAGIGGHIASFASAATLYDVGFNHFWKGHEHPQGADLVYIQGHCTPGIYARAYLEGRL
                     SEAELQRFRMEALAPGLPSYPHTWLKPDFWQFATVSMGLGPIMAIYQARLMKYLQHRG
                     LAPMEGRKVWCFCGDGEMDEPESLGATDIAAREKLDNLVFVVNCNLQRLDGPVRGNGK
                     IIQELEGVFRGAGWNVIKVIWGAAWDPLIAADASGKLLEAFNQTVDGEYQNFKAKGGR
                     YTRENFFGKFPETLRLVETMSDEDIAKLNRGGHDPHKVYAAYAAAMKHTGSPTVILAK
                     TVKGYGMGEAGEGQNVTHQQKKMGEEALRAFRDRFKIPIPDDQVAAAPFYRPAEDSEE
                     IKYLRARRAELGGPLPARVVKPETLEIPPLSALEKITAGTGEREISTTMAFVQILQTL
                     LRDKAIGKRIVPIIPDEARTFGMEGMFRSLGIYSIVGQKYKPEDSEQLAFYKEDIKGQ
                     ILEEGITEAGAMSSWTAAATAYANHNVTLLPFYIFYSMFGFQRIADLAWAAGDMRARG
                     FLLGATAGRTTLNGEGLQHEDGHSHVMAAMVPNCRAYDPAFAYELAVIIQHGMQQMYV
                     EHHDVYYYLSLYNENYAHPSMPTGAESGIVRGMYLLRSAEKAAKTHVQLLGSGSILRE
                     VIAASDLLAADWGVTSDVWSVPSFTELARDGRETERWNLLHPEQAPRQSYVQQCLHGM
                     TGPAIASSDWIRAYAEQIRPYMPLPYHVLGTDGYGRSDNRPALRDFFEIDRRWIVIKA
                     LKALAEQKLLPAERVTQAIKIYGIKPERIAPWKA"
     gene            8486..>9023
                     /locus_tag="VHE37_05685"
     CDS             8486..>9023
                     /locus_tag="VHE37_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVT35051.1"
                     /translation="MEGLSMGASIEVKVPDIGDYHDVPVIEVLVKDGDRVEKDAPLAV
                     LESDKATMEVPAPEAGTVRGIKIKAGDKVSQGHVICSLETAAAAPATKSVAAARSEPA
                     KPAPPKKDASPAPAPPVAAAAGGTIEVKVPDIGDYHDVPVIEVLVKDGDAVEKDAPLA
                     VLESDKATMEVPAPAAGVV"
BASE COUNT         1636 a         2999 c         2897 g         1491 t
ORIGIN      
        1 cgtcgatgcc atcgacatcc ttcgggtagc ggtcacaggt aagaacaatc tgcttgtgac
       61 tgtcgatcag cgcattgaac gtgtggaaaa actcttcctg cgtgcgttcc ttgtgcgcaa
      121 aaaaatggat gtcgtcgatc agcagcgcat cgactgatcg atagaaagtc ttgaactcat
      181 cctgcttgcc gtgacggatg gcggcaacca tgtggttgag ccactgctcc gcacccaaat
      241 aggcgatgcg cgcccgcggc tgtgccttga gaatggtatt gccgatggca tgcatcaagt
      301 gggttttgcc caggccagaa tcgccgtaga tcagcaaggg gttgtaggcg ccgccgggcg
      361 tttccaccac gtgctgcgct gctgcccgcg cctgcgagtt ggacttgccc tcgatgaagt
      421 ttgcaaaggt gtagcgcgga tcgagcttgg cgacgaactg ggccggcgtc tcttccgcgg
      481 tagctggctg cactccgttt acgcgcgcct cacccgcacc gacagcgagt tcaacctccg
      541 gcggattttt cccgccgttg gccttgaccg cgcgtctgat ggtcgcgaga aaatcgttgc
      601 gcacgcgctc cagcacgatg cggttgggcg ccaacagcag gatcttgtcg cgcgaggcta
      661 ccggatgcag tggccggatc caggtggaga tgtccttgtc gggcaactcg gcttccagcc
      721 agcgcacgca ttggatccag atgtcttcgt tctgattttg catgtcgtcg cttgatcctc
      781 gccggcgtcg ggcgacgagg ggcaggcagt ctagcggttt gaccaatact tatccacagg
      841 agtgcgcgaa gccccgtgct tgctgctcga gcagcactcc ggctaaactc cgcgcccttt
      901 tttgcctgcc tttggtgttc cgatgaaacg cacattccag ccgcacaaca agcgccgcaa
      961 acgcacgcac ggttttcgcg ctcgcatggc aacggttggc ggccgcgcga ccttggcccg
     1021 ccggcgcgcc aagggtcgta agcgtctgat cccgtaaaca ccctcggctg aacatggcgc
     1081 gcctgctgtc gcgtgcgcgc ctgcatcgac cggaggattt caaggcggca ctcgagcagg
     1141 gacgacgggt cggaaccggg ctgctgagcg ccgcggtccg gcacaacggt ttgggaattc
     1201 cacggctggg tctggctttg gccaagaaga acatcgcgct ggccaccatg cgaaaccgga
     1261 tcaaacgcca catcagggaa agttttcgcg aacaccaggc ggcgttgcct tcagccgaca
     1321 tcgtcgtgtt tgcgaaggcc tcggcagcac gcgcgaccca ggcggacctg cacgccgcgc
     1381 tcgagcagtt gtggcaacgg gtgaaccaag catgcgccac ctcgccctct ttctgatccg
     1441 cgcctatcag ctgctggtca gcccgttgct cgggccccgc tgccggtttc atcccagctg
     1501 ttcacactac gcggccgagg ccatccatcg ctacggcgtg ctgcgcggca gctggctggc
     1561 gctgcgccgc atcggccgct gcagtccatt gcatcccggc ggctacgatc cgctgccgga
     1621 aaagtaattc aacgagagca cgatggaaaa ccgccgcttc atcctgattg cgctgctcgg
     1681 cgtggtgctg ttcttcatct accaggcctg gcagaccgat taccctccac cggctgtggc
     1741 acccgctgca gcagccgcca gcctgcccgg cgaaagcgcg ccgtcgcccg cacccacttc
     1801 gagttcaacg acaacccttc ctgcagaagc gtccgcgacc gatgttcccg ccacgccgat
     1861 ccaggtgcat accgatctgg tcaacgcgga gatcagcccc aacggcggcg atctggatcg
     1921 cgtggagttg ctcggctacc ccgagttcaa ggatcaaccg gaaaacctgg cgctgctgga
     1981 caaccgcaac ggccgctggt tcgtgttaca gagcggcctc gcatcctccc aggatgcgct
     2041 ggtcacaccg cagagcctgt tcatgtccgc cgcgtccagc taccagctgg cgccgggtac
     2101 ggacacgctc gacgtcacgc tcgagcacgc agcgcccgcg ggataccgcg tgcgcaagac
     2161 gtatcgcttc catcgcggca gctacgaaat cgaattgatc cagaccttgg tcaacggcag
     2221 cgacgccgaa atcagcgcga gtccctgggt gcgctttgcg cgcctgccct acaccgtggg
     2281 ctatcagccc aaattttcca agaccttcgc cggcgaaggc ctgtatgtgc agaaagaagg
     2341 cagcaccagt tatcgcttcg acaagatctc gttcgatgat ttgtcgaaga agccgaccac
     2401 cgtacagcag accggcggct gggtggcgat gctgcagcat tacttcgtcg ccgccatcat
     2461 cccgcccgct gatgaaaaac tgaccctgac ggccaagccg ggcaaggacc gcggatttgt
     2521 cggacagtac gtcggggcgt ttgccacggc gccggtgcat ggcgagcaca gctattccac
     2581 caagctctac atcgggccgg agctgcagga ccgcgtcgcc gacatcgcgc cgggattcga
     2641 gttgacgctg gactacggca tcctcacgcc catcgccaag ccattgttct gggtgctgca
     2701 gctgtatcac cgcctgctcg gcaactgggg cttcgccatc atcctgctca cgctcacggt
     2761 gaagctggcc atgctcaagc tgtcggaagc gcaataccgt tcgatggcca agatgaagaa
     2821 gttcggcccg cgcatccagg aattgaagga acgctatgcc gacgaccgcg agcgccagca
     2881 gaaggcgatg atggatctgt acaagaagga aggcttcaac ccgctggcgg gttgctggcc
     2941 gctcctgctg cagatgccgg tgttcttcgc gttgctatgg gtgctgcagc agtcggtgga
     3001 actgcgcatg gtgccgttcg cgctgtgggt caaggacctg tccgcgcccg atccgtatta
     3061 cgtgctcccg gtgctctacg gtgcggccat gtgggcgcag cagaaaatct ccggccagaa
     3121 cgcggccatg gacccgatgc agcagaagat gatgaacgtg atgccgatcg cgctcaccgc
     3181 gtttttcacc ttctggccgt cgggcctggt gctgtactgg ctggcgtcga cgctgatcgg
     3241 catcgcccag caggcctaca tcacgcgcaa gtacgccgaa gagggcgccg cgcgcgcctg
     3301 acccagcagc agcgcggccg atgcagttcg acgataccat cgccgccatc gccaccgcgc
     3361 cggggcgcgc cgctgtcggc gtggtgcgtc tgtccggtcc gcgtgcgctg agcatcgcgc
     3421 agaaaatctg cggtgacctg ccgccgccgc gcgccgcgag ggtccgcgcg ctgcgcgccg
     3481 cgaacggtgc agtgatcgat cgcggcctgg tgctggtgtt tcccgctccg cattcctata
     3541 ccggcgacga cgtggtggag ctgcaggcgc acggtggccc cgtgctgctc aacctgctgc
     3601 tgcgcagcgc ctgcgagttc ggcgcgcgct tggcgcggcc gggagaattt tcccagcgcg
     3661 cgtttctcaa cgagcgtctc gacctggccc aggccgaagc cgtcgccgac ctgatcgatg
     3721 ccgcgtccga acaggccgcg cgcgccgcgc agcgctcgct cgcaggtgaa ctctcgcaac
     3781 acgtgcacgc gctgacccag cagctgatcg gcctgcgcac ttatgtagag ggcgcgctgg
     3841 atttttccga tgaagatgtc gactggcttg gcgacaccgc ctggcgcgag cggctcgatc
     3901 gcgtgctgcg cgatctcgac cagttgctga agctatgcgc gcagggccgg cgcctgcgcg
     3961 aaggcatggt ggtggcgttg tccggccagc ccaacgtcgg caagtccacg ctgctcaacc
     4021 ggctggccgg cagcgacagc gccatcgtca ccgacatcgc cggcaccacg cgcgacgtgc
     4081 tgcgcgaaca catcgtcatc ggcggcatgc cggtcacgct ggtcgacacc gccggcctgc
     4141 gccacagcga cgacccggtg gaagccgaag gcatccgccg cgcctggtcc gccgtggccg
     4201 atgccgaact gaccttgttc atggtcgatg atcgcgcggg catcacggcc gccgaccgcg
     4261 acctgctcgc gcgcatgggc gccggcgcgc gcgtgctcat cgtgcgcaac aagtgcgacc
     4321 tgtccgcgcg tgttgcggag attttcgagc aggacggctg gacgcagctg cgcctgtcgg
     4381 ccaccaccgg cgacggcgtc gacctgctgc gcgcgcgcat cgaatacgcg gccggtctcg
     4441 acgcgcatgg cgaaggcgtg ttcagcgcac gtgcgcgcca cctggatgcg atgcacgctg
     4501 cgcgagcgca tctgcagcgc gcggcacaac acatcgcggc gcattcgtca gccgaactcg
     4561 ccgccgaaga actgcgactg gcgcagcagg cgctgaacga aatcaccggc gaattcacca
     4621 gcgacgacct gctcggccgc atcttcggca gcttctgcat cggcaagtga gcggccgcgc
     4681 gccgctttga gctaaggtgc gcggcatgga cgccgccgcg ctcaccgcgt acctgcatca
     4741 gcacatcccg ctcacgcgcg cgctcggcgc gcgggtggaa agctgcggac acggcagcgc
     4801 cgtggtgagc gcgccgctcg agcccaatct caaccatcgc aacaccgcgt tcggcggcag
     4861 cctggccacg ctcgccatcc tgtccggctg ggtggtgctg cacgttgcac tgcgcgaagc
     4921 cggcatcgac gcgcgcctgg tggtgcagaa gcacgagtgc gaattccagc agccggtcac
     4981 cgcagcgttc accgcgcgca gcacgctgcc gccgcgggac tgggaaaaat tccgcgccgc
     5041 gctgcagcgc cgcggccgcg cacgcatcag cgtggtcagc gagttgcgca gtggcgacgc
     5101 gcttgtggtc caatccaccg gaacctttgt cgcgctcaca tgaaaccgta caagttctgc
     5161 ccgcagtgcg gcgctccgct cagcagcggc cagcacggcg gtttcgcgcg catgggctgc
     5221 accgcccagg gttgtggctt cgtgcactgg gacaacccgg tgccggtggt cgcggccatc
     5281 gtcgagcacg agggcggcat cattctggcg cgcaataaat tgtggccgat gaagttctac
     5341 gggctgatca ccggctttct ggagaagaac gatccgtcgc ccggcgaagc ggcggtgcgc
     5401 gaggtgaagg aagaactcgg cctcaatggc ggcgcgccca ccttcgtcgg gcattacccg
     5461 ttcgagcgca tgaaccagat catcatcgcg ttccacgtgc cggccgccgg cgagatcgtg
     5521 ctcggcgagg aattgtccga gtacaagcgc atcgcgttcg gcgaggcgcg ctactggcct
     5581 gcgggcaccg ggcttgcgct gcgcgactgg ctgcgcacgc gcggctacga tccgcagccg
     5641 atggagatgc ccaagcctcg ctgaagcgtt aaaaaaacca ccaccgcacc gcccaaaacc
     5701 atgttatata ttattgtagt aattgaatat taaacaataa tttataacca gtccaaatag
     5761 ggggcggtga agcaaaaatg gcccgctaga atcgccagca agctgatcga accactcgaa
     5821 ccaccatgac cgccgaaccg cctgcctcgc ccgccgacct cgacccacag gaaacccgcg
     5881 agtggctcga cgcgctcgac gcggtgctcc agcgcgtcgg acccgagcgc gcgcatttcc
     5941 tgctcgagac gctgagcgag aaggcgcggc gcaccggcgc gttcattccg ttctccgcca
     6001 acacggcgta cctcaattcc attccgccgc atctcgagga gcgctcgccg ggtgaccacg
     6061 cgctggaatg gaagatccgc tcgatcatcc gctggaacgc gatggccatg gtggtcaacg
     6121 ccaaccgcga gcacgctggc atcggcgggc acatcgccag cttcgcctcg gccgccacct
     6181 tgtacgacgt cggcttcaac catttctgga aaggccacga gcatccgcag ggcgccgacc
     6241 tggtctacat ccagggccac tgcacgccgg gcatctacgc gcgcgcgtat ctcgaaggac
     6301 gtctgagcga ggctgaactg cagcgcttcc gcatggaggc gctggcgccg ggcctgccct
     6361 cgtatccgca cacctggctc aagccggact tctggcagtt cgccaccgtg tcgatgggcc
     6421 tggggccgat catggcgatc tatcaggcgc gcctgatgaa gtacctgcag caccgcggac
     6481 tggcgccgat ggaaggccgc aaggtgtggt gcttctgcgg cgatggcgag atggacgagc
     6541 ccgagtcgct cggcgccacc gacatcgccg cgcgcgagaa gctcgacaac ctggtgttcg
     6601 tggtcaactg caatctgcag cgcctcgacg gcccggtgcg cggcaacggc aagatcatcc
     6661 aggaactcga aggcgtgttc cgcggcgccg gctggaacgt gatcaaggtg atctggggcg
     6721 cggcctggga cccgctgatc gcggcggacg ccagcggcaa attgctcgag gcgttcaacc
     6781 agacggtcga cggcgagtac cagaacttca aggccaaggg cgggcgctac acgcgcgaga
     6841 atttcttcgg caagtttccg gaaacgctgc gcctggtcga aaccatgagc gacgaggaca
     6901 ttgccaagct caaccgcggc ggccacgatc cgcacaaggt ctacgccgct tatgcggcgg
     6961 cgatgaagca caccggcagc ccgacggtga tcctggccaa gacggtcaag ggctacggca
     7021 tgggcgaagc tggcgaaggc cagaacgtga cgcatcagca gaagaagatg ggcgaggaag
     7081 cgctgcgcgc cttccgcgat cgcttcaaga ttccgatccc cgacgaccag gtcgccgccg
     7141 cgccgttcta ccggccggcc gaggactccg aggagatcaa gtacctgagg gcgcgccggg
     7201 ccgagctcgg cggcccgctg ccggcgcgcg tggtcaagcc ggagacgctg gagattccgc
     7261 cgctgtccgc gctggagaaa atcaccgccg gcaccggcga gcgcgaaatt tccaccacca
     7321 tggcgttcgt gcagatcctg cagacgctgc tgcgcgacaa ggccatcggc aagcgcatcg
     7381 tgccgatcat tcccgacgag gcgcgcacct tcggcatgga aggcatgttc cgctcgctgg
     7441 gcatctattc catcgtcggc cagaaataca agcccgagga ttccgagcag ctggcgttct
     7501 acaaggaaga catcaagggg cagattctgg aagagggcat caccgaggcc ggcgccatgt
     7561 cgagctggac cgcggcggcc acggcgtatg ccaaccataa cgtcaccttg ctgccgttct
     7621 acatttttta ttcgatgttc ggcttccagc gcattgccga tctcgcctgg gcggccggcg
     7681 acatgcgcgc gcgcggcttc ctgcttggcg ccacggctgg ccgcaccacg ctcaatggcg
     7741 aaggcctgca gcacgaggac ggccacagcc acgtgatggc ggccatggtg cccaactgcc
     7801 gtgcctacga tccggccttc gcctacgagc ttgcggtgat catccagcac ggcatgcagc
     7861 agatgtatgt cgagcaccac gacgtctact actacctgtc gctgtataac gagaactacg
     7921 cgcatccgtc catgcccacc ggcgcggaga gcggcatcgt gcgcggcatg tacctgctgc
     7981 gctcggccga gaaggcggcg aagacgcacg tgcagctgct cggttccggc tcgatcctgc
     8041 gcgaggtgat cgccgcatcc gacctgctcg ctgccgactg gggcgtgacc agcgatgtgt
     8101 ggagcgtgcc gagcttcacc gagctggcgc gcgacggccg cgagaccgag cgctggaacc
     8161 tgctgcaccc cgagcaggcg ccgcggcaaa gctacgtgca gcaatgcctg cacggcatga
     8221 ccggccccgc catcgcgtcg agcgactgga tccgcgccta tgccgagcag atacgcccgt
     8281 acatgccctt gccctatcac gtgcttggca ccgacggcta cgggcgctcc gacaatcgcc
     8341 cggcattgcg cgacttcttc gagatcgacc gccgctggat cgtgatcaag gcgctcaagg
     8401 cgctggccga acagaaactg ctgccggccg agcgcgtgac gcaggcgatc aagatctacg
     8461 gcatcaagcc ggagcgcatc gctccgtgga aggcctgagc atgggcgcat cgatcgaagt
     8521 gaaggtgccc gacatcggcg actaccacga cgtgccggtg atcgaggtgc tggtcaagga
     8581 cggcgaccgc gtggagaagg acgcgccgct ggcggtgctg gaatccgaca aggccaccat
     8641 ggaagtgccg gcgccggaag ccggcacggt gcgtggcatc aagatcaagg cgggcgacaa
     8701 ggtgtcgcag ggccacgtga tctgcagcct ggaaaccgcg gccgccgcgc ctgctacaaa
     8761 atccgtagca gcggcccgca gcgagccggc gaaaccggcg ccgccaaaga aagacgcgtc
     8821 gcccgcccca gctccgccag tcgccgccgc cgctggcgga acgatcgagg tgaaagtgcc
     8881 cgacatcggc gactaccacg acgtgccggt gatcgaagtg ctggtcaagg acggcgacgc
     8941 ggtggagaag gacgcgccgc tggcggtgct ggaatcggac aaggccacca tggaagtgcc
     9001 cgcgcccgcg gccggcgtgg tgc
//
LOCUS       DASZZC010000267         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_535636,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000267 DASZZC010000000
VERSION     DASZZC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535636"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VHE37_05690"
     CDS             <1..294
                     /locus_tag="VHE37_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35052.1"
                     /translation="GLTAEMLLAGKPGVLLPDTLERALMARRAVQLGAALAPPENGEF
                     NLSAALRTVLTDSRLANAARAFARRHHHLDRAQILPRMADRILDRVSFPAPAA"
     gene            complement(269..1444)
                     /locus_tag="VHE37_05695"
     CDS             complement(269..1444)
                     /locus_tag="VHE37_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HVT35053.1"
                     /translation="MTASSHPIHDVIVVGGGVVGAALTLALQRHGYDVVMVERGAAPR
                     SWDASRYDLRVYALSPASIALLDELGAWAPMRAARVSPYRRMRVWDDDPAQALVFDAA
                     DARAAQLGCIVENELMLSCLWQLLPSASIRTASAVTALQVSDAHADVTLADGAVLRAR
                     LVIATDGADSPLRQMAGIEAQGWSYPQHALVCHVQTEKPHDGACWQRFTPAGPLAFLP
                     LADGRSSIVWSSREADTLAALDDAAFCARLEEASQQQLGHIGGCTARMRFPLRLLHAE
                     RYVAPRLALAGDAAHVVHPLAGQGVNLGLADVRALLQVLDEARGAARDIGGLRVLKRY
                     ERARRADNLDMLALTDALYRAFDLRLPAWNGMRALGLAAVNRLLPLRQLFMQRALGS"
     gene            1510..1812
                     /locus_tag="VHE37_05700"
     CDS             1510..1812
                     /locus_tag="VHE37_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35054.1"
                     /translation="MFEHGCSSRQDFTVPEAQHPESFAVQKSGAEFILPPIDMLATVE
                     LDDQAAFQADEIGDVFADRLLAAKLAAAELPAPRITPQVQPGIGLRTAQISRQIVA"
     gene            complement(1828..>2310)
                     /locus_tag="VHE37_05705"
     CDS             complement(1828..>2310)
                     /locus_tag="VHE37_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVT35055.1"
                     /translation="ARFRAAAHETFGERLGDFCELGKRNAHPLSRVLSQQLTAPRVVF
                     AGNAAQSLHPVAAQGFNLGLRDVAALGEALRDAADPGAPELLEAYAARRARDREATAG
                     FTDHLVRLFSNRVPGLSALRHLGLLALDLAPPLKQAVMWQNLGSTGYVPSPPGREGQG
                     "
BASE COUNT          370 a          826 c          798 g          316 t
ORIGIN      
        1 ggcctcaccg cggaaatgct gctggctggc aagcccggcg tgctgctgcc ggacacgctg
       61 gagcgtgcgc tgatggcacg ccgcgcggtg cagctgggcg cggcgctggc gccgccggaa
      121 aacggggagt tcaacctgag cgccgcgttg cgaacggtgc tgaccgactc gcgcctggcc
      181 aacgcggcgc gcgccttcgc ccggcgccat caccacctcg accgcgcgca gatcctgccg
      241 cgcatggccg accgcatcct cgatcgcgtc agcttcccag cgcccgctgc atgaacagct
      301 gccgcagcgg cagcagccgg ttgaccgcgg ccaagcccag cgcgcgcatg ccgttccacg
      361 ccggcaggcg caggtcgaac gcgcgataga gcgcgtcggt cagcgccagc atgtcgaggt
      421 tgtccgcgcg ccgcgcacgc tcgtagcgct tgagcacgcg cagcccgccg atgtcgcgcg
      481 cggcaccgcg cgcctcgtcg agcacctgca gcagcgcgcg cacgtcggcc agcccgagat
      541 tgacgccctg cccggccagc ggatgcacca cgtgcgcggc atcgccggcc agcgccagcc
      601 gcggcgccac gtagcgctcc gcatgcagca ggcgcagcgg gaagcgcatg cgcgcggtgc
      661 agccgccgat gtgtccgagc tgttgttgcg acgcttcttc cagccgcgcg caaaacgctg
      721 catcgtcgag cgccgccagc gtgtcggcct cgcgcgacga ccagacgatg gatgagcgcc
      781 cgtccgccag cggcaggaag gccagcggcc cggccggggt gaagcgctgc cagcaggcgc
      841 cgtcatgcgg cttttccgtc tgcacgtggc acaccagcgc gtgctgcggg tagctccagc
      901 cctgcgcctc gatgccggcc atctggcgca gtggcgaatc ggcaccgtcg gtggcgatga
      961 ccaggcgcgc gcgcagcacc gcgccatcgg ccagcgtcac gtccgcatgc gcgtcgctca
     1021 cctgcagggc agtcaccgca ctcgcagtcc tgatcgatgc agacggcagc agctgccaca
     1081 ggcacgacag catcagttcg ttttccacga tgcagcccag ctgcgccgcg cgcgcatcgg
     1141 cggcgtcgaa caccagcgcc tgcgccgggt cgtcgtccca cacgcgcatg cggcggtacg
     1201 ggctcacgcg cgccgcgcgc atcggcgccc aggcaccgag ttcatcgagc agcgcgatcg
     1261 atgccggaga cagtgcataa acgcgcaggt cgtagcgcga tgcatcccag ctgcgcggcg
     1321 cggcgccgcg ttccaccatg acgacgtcgt agccgtggcg ctgcagcgcc agcgtgagcg
     1381 cggcgccgac cacgccgccg ccgactacga tcacgtcgtg gattgggtgc gatgaagcgg
     1441 tcatggctct tgagccctct acccctgggg gagagggttg ggtgaggggt tggtcagagc
     1501 caacaggatt tgttcgagca cggctgcagt tcgcgtcagg acttcaccgt tccagaagcg
     1561 cagcacccgg aatcgttcgc ggttcaaaaa tccggtgcgg aattcatcct gccgccgatc
     1621 gacatgctgg ccaccgtcga gctcgacgat caggcggctt tccaggcaga cgaaatcggc
     1681 gatgtatttg ccgatcggct gctggcggcg aaacttgcag ctgccgaact gccggctcct
     1741 cgaataacgc cacaagtgca gcccggcatc ggtctgcgta cggcgcagat ttctcgccag
     1801 atcgtagcgt agtggacgag tcatccccta accctgaccc tctcttccag ggggagaggg
     1861 aacgtagccc gtggaaccca ggttctgcca catgaccgcc tgcttgagcg gcggcgccag
     1921 gtccagcgcc agcaggccga gatggcgcag cgccgacagg ccgggcacgc ggttggagaa
     1981 caggcgcacc aggtgatcgg taaagccggc ggtcgcctcg cggtcgcgcg cgcggcgcgc
     2041 ggcgtaggcc tcgagcaatt ccggcgcgcc gggatcggcc gcatcgcgca gcgcctcgcc
     2101 cagggcggcg acatcgcgca ggccgagatt gaagccctgc gccgccaccg ggtgcagcga
     2161 ctgcgcggca ttgccggcga acaccacgcg cggcgcagtc agctgctgcg acagcacgcg
     2221 cgacagcggg tgcgcattgc gcttgcccag ttcgcagaaa tcgccgagcc gctcgccgaa
     2281 ggtttcatgc gcggcagcgc ggaagcgcgc
//
LOCUS       DASZZC010000268         4521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_472513,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000268 DASZZC010000000
VERSION     DASZZC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472513"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VHE37_05710"
     CDS             complement(<1..1282)
                     /locus_tag="VHE37_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35056.1"
                     /translation="MGPGNFRGIAGCAAAWLLLAGGRAAAQDARPVAAPAEARAQAQT
                     LDDVVVTARRRQERSLDVPISLAVLDGQALGEAGLTLPTDIQERVPGLVVSVPNARLT
                     TYTIRGLGSSSANDGIESSVGLFLDGVYLGRQGLSIFDLIDLERVEILRGPQGTLFGK
                     NTTAGAISIVTRAPTDQYEGNVEGTVGSIGARQLRAAINGPLLDGTLAGRLTGYVSRR
                     DGTVFNVYDGNTLNDRNKFGLRGQLLWTPRADLSVRFIAEHAEQHENCCVFPLTAPVR
                     AAVQARDDYMEYLRVGTNPYDHLVDSDAPTRSDQRQQALSAELNWDLGALGQLTSISA
                     WRDWHFVPLNDDATSLRLASTSTGDHHRQLSEELRLASSFDHVDVVGGLYYLHQHLDG
                     LERVILGKDTVGWVFGGLIRQKVLPFATQSNTGPA"
     gene            1345..1791
                     /locus_tag="VHE37_05715"
     CDS             1345..1791
                     /locus_tag="VHE37_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HVT35057.1"
                     /translation="MKFGLGKWFGVQPPSTQPHDEALAVAQLLLEIARADLATEPAEL
                     AAIRAHLQQAYALSDAQLDPLMAHAQRQVEQSVSMADSLKQVNAALDPPRKAQLMRAL
                     WQVAYADGRIDPHEEAMLRRLADLLYVPHSVFIREKLGVAEGPRHG"
     gene            1784..2305
                     /locus_tag="VHE37_05720"
     CDS             1784..2305
                     /locus_tag="VHE37_05720"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HVT35058.1"
                     /translation="MADALSARRVDITTLALDAIVNAANESLLGGGGVDGAIHRAAGP
                     QLLGECRTLGSCATGDAKITRGYRLPARHVIHTVGPVWRGGGRGEAQLLASCYRRCFQ
                     ISSTHGIASIAFPAISCGVYGYPPEQAVHIAVGQTRRHLARANLPSSVLFACFSDEVL
                     ALYEAEIRLTAPA"
     gene            2380..2649
                     /locus_tag="VHE37_05725"
     CDS             2380..2649
                     /locus_tag="VHE37_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35059.1"
                     /translation="MIFTSIRKQGGAGILLMLLAGAAQAQGCCSHHGGIKGCDAKSGM
                     LMCEDKSLSAKCTCKYWQRQQPQNMNGRQPPTETSTHHDAEKAKQ"
     gene            2998..3591
                     /locus_tag="VHE37_05730"
     CDS             2998..3591
                     /locus_tag="VHE37_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HVT35060.1"
                     /translation="MLLFSAGFLAQLALMLLALLFAQFAGRNVGDVERCLYICFTWLN
                     IFLVVTNLIPGQVAGGLANDGLVLWRLFLHAYRNGPHPHPRLEAKSADVAPVFSPETR
                     LLSMPTLIPSGFRDGIEILNDRTTPMELVVTTLMKHLELSRDEAIVKMLAIHNNGGLL
                     VPLPTKAWAEQVATAISSDAKLAGHQLICRAVSSANE"
     gene            3633..>4521
                     /locus_tag="VHE37_05735"
     CDS             3633..>4521
                     /locus_tag="VHE37_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HVT35061.1"
                     /translation="MSTLQLFATCPRGVENLLAAELAALGAISAKERKGGVAFGGELA
                     VAYRACLWSRVASRVLLPLSTFPLTDADALYAGARAIDWTAVFGAQATFAIEVAGNSP
                     ALTHTHYAGLKVKDAIADVFREKTGARPDVDTERPDIRVHLHLERAQATLSLDFAGDS
                     LHRRGYRMRGGEAPLKENLAAAILLRAGWPALAREGAALLDPMCGSGTLVIEAALMAA
                     DIAPNLARARFGFESWLGHDDALWREVRADAQSRRSAGLARALPPMRGADRDAQALKL
                     ARASAERAGFTTQIEWSEGD"
BASE COUNT          751 a         1524 c         1473 g          773 t
ORIGIN      
        1 gcgcgggccc ggtgttggac tgcgtggcga acggcagcac cttctgccgg atcaggccgc
       61 cgaataccca gccgacggtg tccttgccca ggatcacgcg ctccaggccg tcgagatgct
      121 ggtgcaggta gtacagtccg ccgaccacgt cgacatggtc gaagctcgac gccagccgca
      181 gttcctcgct cagctgccgg tggtggtcgc cggtactggt cgaggccagc ctgagtgacg
      241 tggcgtcgtc gttgagcgga acgaaatgcc agtcgcgcca cgcgctgatg ctggtcagct
      301 ggcccagcgc gccgaggtcc cagttcagtt ccgccgacag cgcctgctgg cgctggtcgc
      361 tcctggtcgg cgcgtcggaa tcgaccagat ggtcgtaggg attggtgccg acgcgcaggt
      421 attccatgta gtcgtcgcgc gcctgcaccg ccgcgcgcac cggcgcggtc agcgggaaca
      481 cgcagcagtt ctcgtgctgt tcggcgtgtt cggcgatgaa gcgcaccgac aggtccgcgc
      541 gcggcgtcca cagcagctgc ccgcgcaacc cgaacttgtt gcggtcgttg agcgtgttgc
      601 cgtcgtagac gttgaacacg gtgccgtcgc ggcggctgac gtagccggtg agccggccgg
      661 ccagcgtgcc atcgagcagc gggccgttga tggcggcgcg cagctggcgc gcaccgatgc
      721 tgcccacggt gccctcgacg ttgccttcgt attgatcggt cggcgcgcgg gtgacgatgc
      781 tgatggcgcc cgccgtggtg ttcttgccaa acagcgtgcc ctgcgggccg cgcaggatct
      841 cgacgcgctc caggtcgatc aggtcgaaga tcgacaggcc ctgccggcca aggtacacgc
      901 cgtcgagaaa caggccgacg ctgctctcga tgccgtcgtt ggccgacgac gagcccaggc
      961 cgcggatggt gtaggtggtg agccgcgcat tgggcaccga taccaccagc cccggcacgc
     1021 gctcctggat atcggtgggc aaggtcaggc cggcctcgcc gagcgcctgg ccgtcgagca
     1081 ccgccagcga gatcggcacg tcgagcgagc gttcctggcg gcggcgcgcc gtcaccacca
     1141 cgtcgtccag cgtctgtgcc tgtgcccgcg cttccgccgg tgcggcgacg ggccgcgcgt
     1201 cctgcgccgc ggcgcgcccg cccgccagca gcagccacgc cgctgcgcac cccgcaattc
     1261 ccctgaagtt ccccggcccc atgaggcgca tcttacaaag agcccggccg gcacatgcgc
     1321 ctgcaccacg ggtaaacttt cctcatgaaa ttcggcctcg gcaaatggtt cggtgtgcag
     1381 ccgccgtcaa cgcagcccca cgacgaagcg ctcgcggtgg cgcagctgct gctggaaatc
     1441 gcgcgcgccg acctggccac tgagccggcc gagctggcgg cgatccgcgc gcacctgcag
     1501 caggcctacg cgttgtccga tgcgcagctc gatccgctga tggcgcacgc gcagcggcag
     1561 gtcgagcagt cggtgtcgat ggccgacagc ctcaaacagg tgaacgctgc gctcgatccg
     1621 ccgcgcaagg cgcagctgat gcgcgcgctg tggcaggtgg cttatgccga cggccgcatc
     1681 gatccgcacg aggaagcgat gctgcgccgg ctggccgacc tgctgtacgt gccgcactcg
     1741 gtattcatcc gcgaaaagct cggcgtggcc gaaggcccgc gccatggctg atgcattgag
     1801 cgcgcggcgc gtggacatca ccacgctggc gctcgatgcc atcgtcaacg ccgccaacga
     1861 gtcgctgctc ggcggcggcg gcgtggacgg cgccatccac cgcgccgccg gcccgcagct
     1921 gctgggcgag tgtcgcacgc tgggcagctg cgccaccggc gatgcgaaga tcacccgcgg
     1981 ctatcgcctg ccggcacgcc acgtcatcca cacggtgggc ccggtgtggc gcggcggcgg
     2041 ccgcggcgaa gcgcagctgc tggcctcgtg ctaccgccgc tgcttccaaa tcagtagcac
     2101 gcacggcatc gccagcatcg cctttcccgc catcagctgc ggcgtgtacg gttacccgcc
     2161 tgagcaggcg gtacacatcg cggtgggcca gacgcgccgg cacctggcgc gcgccaacct
     2221 gccgtcgagc gtgctcttcg cctgcttcag cgacgaggtg ctggcgctgt acgaggccga
     2281 aatccgcctc accgcaccgg catgattgat gttactattt atctatttaa tttaaaatta
     2341 gacaatattc aataacaaca aaaacggggg tgcggtgaca tgattttcac gtccatccgg
     2401 aaacaaggcg gtgccggaat cctgctgatg ctgctcgccg gcgccgcgca ggcgcagggc
     2461 tgctgctcgc accacggcgg catcaagggc tgcgatgcga agagcggcat gctgatgtgc
     2521 gaggacaagt cgctgtccgc caaatgcact tgcaaatact ggcagcgaca gcagccccag
     2581 aatatgaacg ggcggcagcc gcccactgaa acctccacac atcacgatgc ggaaaaggca
     2641 aaacaatagt gtcgctccac ggcatgcttc ccaccggaac cttgtaattg gccgctgata
     2701 cgaaatccgt caacggtaca cggcgacccg gatacgtccg cctggcaaca ttcggcggag
     2761 ttccggttta tgcacatcgg tctttgccga tcctgggcgc gtgcgcgttg ttgctgtacc
     2821 aatacagcca gcatttgtcg gtggtcgcat ttctcggcta catgatcgtc attgcattgc
     2881 acgaacttgg ccatgcactc gttgcccgtc tgctgaaaac taagactatt ttcggtggaa
     2941 ctttctgcgc tcggaggcgt ttgccgaacc gaacggccac gcgaggtccg acatagcgtg
     3001 ctgttgtttt ctgccgggtt tcttgcgcaa ctcgcgctga tgcttctcgc tttactcttc
     3061 gcccagttcg ccggcagaaa cgtcggcgac gtcgaacgtt gtctgtacat ttgctttacc
     3121 tggctaaata tctttctcgt ggtgaccaac ctcatacccg gccaggtcgc gggcggactt
     3181 gcaaatgatg ggttggtgct gtggcgccta tttctgcacg catatcggaa tggaccacat
     3241 cctcatcctc ggctggaagc gaaatccgcc gacgttgcgc cagttttttc gcccgaaacg
     3301 cgcttgctgt ctatgccgac gttgattcct tcgggcttca gggacggaat tgaaatcctg
     3361 aatgatcgca ccacacccat ggaactggtc gtcactacgc tgatgaaaca cctggagctg
     3421 agtcgagatg aggccatcgt caagatgctt gccattcata acaatggcgg acttctagtg
     3481 ccgcttccca ccaaagcatg ggctgagcaa gttgccaccg cgatttcttc cgatgccaag
     3541 ctcgccggac accaattgat ttgtcgtgca gtcagcagtg caaatgaatg aactcgccag
     3601 cgcgttaaca gaggtctgcg agataatggc gcatgagcac gctgcaactg ttcgccacct
     3661 gtccgcgtgg cgtggaaaac ctgctggccg cggaactggc ggcgctcggc gccatcagcg
     3721 cgaaggagcg caagggcggc gtcgccttcg gcggtgaact cgccgtcgcc taccgcgcct
     3781 gcctgtggtc gcgcgtcgcc agccgcgtgc tgctgccgct gtcgaccttt ccgctgaccg
     3841 acgccgacgc gctgtacgcc ggcgcgcgcg ccattgactg gaccgccgtg ttcggcgcgc
     3901 aggccacttt cgccatcgag gtggccggca actcgcccgc gctcacccac acgcattacg
     3961 ccgggctcaa ggtgaaggac gccatcgccg acgtgttccg cgagaaaacc ggcgcgcgcc
     4021 ccgacgtgga caccgagcgg ccggacatcc gcgtgcacct gcacctggag cgcgcgcagg
     4081 ccacgctgtc gctcgacttc gccggcgaca gcctgcaccg ccgcggctac cgcatgcgcg
     4141 gcggcgaggc gccgctgaag gaaaacctgg cggccgccat cctgctgcgc gccggctggc
     4201 cggcgctggc gcgcgagggc gcggcgctgc tcgaccccat gtgcggctcc ggcacgctgg
     4261 tgatcgaggc ggcgctgatg gccgccgaca tcgcaccgaa ccttgcgcgc gcgcgcttcg
     4321 gcttcgaaag ctggctcggc cacgacgacg cgctgtggcg cgaggtgcgc gcggacgcgc
     4381 agtcgcgcag gagcgcgggc ctggcgcgcg cactgccgcc catgcgcggc gccgaccgcg
     4441 acgcgcaggc gctgaagctc gcacgcgcga gcgccgagcg cgccggcttc accacgcaga
     4501 tcgagtggag cgagggcgac g
//
LOCUS       DASZZC010000269         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1179212,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000269 DASZZC010000000
VERSION     DASZZC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179212"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2221)
                     /locus_tag="VHE37_05740"
     CDS             complement(170..2221)
                     /locus_tag="VHE37_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HVT35062.1"
                     /translation="MSAGNREFSVAMRLLLEAQGIKPGAQAAKKELTEAASAGAKGFE
                     KVDAQVTKVGQTIAYKLGTSLQVSQATFQKMGMEGTRTFSSWTQGANNAYAATDRLTN
                     STERARRNLRGIREEASSTFRFLQDSQRRMGSLFGMLGFAGAGYGAFRLAAQSGRLDQ
                     DTTRVMQTADATPAQRDQLRKDLFALSRRTGRPIDELLDVQNTFVQAGLDWGKASAAT
                     SAVAPAMAVSGASGQGLARGLVTASNLYGYDLSKPGVAAMLLDQMRAAGKAGNAEMEQ
                     MSDILPRVAPKAREAGMDFSKTLSFVEMFSQIVPEPEKLATLSESTLRLWSTFQYRAR
                     MEQLTGTKFFDAKGATREPASVLADLIGFASKGKTPEERANRLGAMFYGMDQDTATGA
                     AMLIQDPKKLDKMDSIAAAIANSRGMVESELPGSMDNIDAQSGRLKASMRQAADAFAQ
                     PINSVLKDLIKWSQDTAKMGPMATTASTLAVAAGAVGAGTLLAAGARRLRGGLFASAA
                     GTAVGIAEGKALQMAAGVTPVFVTNWPGGGSSGGGDLATGVVGGAVAGRVIKGLPAAV
                     RLLRAATVADLFGGALGLGGASIAGAGLLGAGAVGYGVGSVIHSLLPDDLSDKIGHLE
                     ATLLALFGDRTARSGLERESRAKLELTIKDGGAAVTGFTSSGGLDLSVYGAWLLNGAR
                     P"
     gene            complement(2367..>2707)
                     /locus_tag="VHE37_05745"
     CDS             complement(2367..>2707)
                     /locus_tag="VHE37_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35063.1"
                     /translation="MKAKTPFELVAADPVTIRGPLRFGCELKGKKATSFVMREATTDD
                     VLAAEADAHAVNTPLTYEVALAARQLVSIGDFEGPFTLGVLREKLSKPADIQRLRMAQ
                     VEIDRLVGEG"
BASE COUNT          443 a          914 c          841 g          509 t
ORIGIN      
        1 taggcatcga ggcggaaggc tgtgggcgcc gcgcccatgt cttcggtctc gggcagatcg
       61 cgacctggat attcatgaac gacgacgcgc cggcctatca ctagctcatg cgcgtcgatc
      121 aggaacttca cgccgcggaa actcgcgtcg agcaactggt cgcgccagct catggacgcg
      181 ctccgttcag gagccaggca ccgtagacgc tgaggtcaag acccccgctg ctggtgaaac
      241 cggtgacggc ggcgccgcca tctttaatgg ttaattcaag cttcgcacgg ctttcgcgct
      301 caagcccgct gcgcgcggtt ctatcgccga acagtgccag cagggtcgcc tccagatgcc
      361 cgatcttgtc gctgagatcg tcgggcagta gggaatgaat gaccgagccg acgccgtatc
      421 caacagcgcc tgcgccaagc aagccggccc ctgcgattga cgcgccgccc aatcccaagg
      481 cgccgccaaa tagatccgct acggtcgctg ctctcaacag gcgcaccgcg gcaggcaggc
      541 ccttgatcac gcggccagcg acggcgccgc cgacaacgcc ggtcgccagg tcgccgccgc
      601 cagaagaccc gcccccgggc caattggtca cgaacaccgg ggtaaccccg gcggccatct
      661 gtagcgcctt gccctcggca atccccactg cggtgccagc agcgctagca aacaatccgc
      721 cgcgcaggcg ccgcgcaccg gcggccagta atgtcccggc acccacggcg ccggcggcga
      781 cggccagcgt gctcgcggta gtcgccatcg gtcccatctt cgccgtgtcc tgagaccact
      841 taatgaggtc tttcaggacg gagttaatcg gctgagcgaa agcatctgca gcctgacgca
      901 ttgacgcctt gagccggcct gactgggcgt cgatgttgtc catcgaaccc ggaagttcac
      961 tctcgaccat gccccgcgag ttcgcgatgg cagcggcgat gctgtccatc ttgtccagct
     1021 tcttgggatc ctggatcagc atcgccgcgc ccgtcgcggt gtcctggtcc atgccataga
     1081 acatggcgcc cagccggttt gcccgctctt ctggtgtctt gcccttcgag gcaaagccaa
     1141 tgagatccgc cagcacggat gctggttcgc gcgtcgcacc tttggcgtcg aagaacttgg
     1201 tgccggtgag ctgctccatg cgggcgcggt actgaaaggt gctccacagg cgcagcgtgc
     1261 tttcgctgag cgtggccagc ttttccggct cgggcacgat ctgcgagaac atctcaacga
     1321 aactcagtgt tttcgagaaa tccattcccg cttcgcgtgc cttgggcgcc acgcgcggca
     1381 agatgtccga catctgctcc atttccgcgt taccggcctt gcccgcggcg cgcatctgat
     1441 cgaggagcat ggccgccact cccggcttgg agaggtcgta gccgtataga ttcgaagcgg
     1501 tcaccaggcc acgggccagg ccttgaccag atgcgccgct caccgccata gctggggcca
     1561 cagccgaggt ggcagcgctt gccttgcccc agtccaggcc cgcctgcacg aaggtgtttt
     1621 ggacgtcgag cagctcgtcg atcgggcgcc cggtccgtcg agacagcgca aacaagtcct
     1681 ttcgtagttg atcgcgttgc gcgggagtcg catctgccgt ctgcatcacc cgcgtggtgt
     1741 cctggtccag gcgccccgat tgagcggcca ggcggaaagc tccatagccc gcgccggcaa
     1801 agcccagcat cccgaagagg ctgcccattc gacgttggct gtcctgcagg aagcggaaag
     1861 tgctgctcgc ctcttcgcgg attccacgca gattgcgccg ggcgcgctcg gtggaattcg
     1921 tcagccggtc ggtggctgca taggcattgt tggcgccctg cgtccagctg ctgaacgtcc
     1981 gtgtgccctc catgcccatc ttctggaagg tggcctgcga cacctgcagg ctggtgccga
     2041 gcttgtaggc gatggtctgg ccaaccttgg tgacctgggc gtcgactttt tcgaagccct
     2101 tggcgccggc gctagccgcc tcggtgagct ccttcttggc cgcctgggcg cccggcttga
     2161 tgccctgcgc ttcaagcaat agccgcatgg cgacggagaa ttcgcggttt ccggcactca
     2221 tggttttcta ggcttccgaa attccccgat cagctcgatg cattcgtgca gctctccgcg
     2281 cggcattgcg cggacttcgg tgcggctcca gccggtgaac cggctcagga tcagcaccgc
     2341 gccgaggagc tccttgcggc gcgggcttac ccttccccca ccaggcgatc gatctccact
     2401 tgcgccatgc gtagtcgctg gatgtcagca ggcttgctca acttttcgcg cagcacgccc
     2461 agcgtaaacg ggccttcgaa gtcgccgatc gacaccagct ggcgcgcggc gagcgccacc
     2521 tcgtaagtca gcggcgtatt gacggcatga gcgtcggcct cggccgcaag cacgtcgtcc
     2581 gtggtggcct cgcgcatgac gaagctggtg gccttttttc ctttcagctc gcagccgaac
     2641 cgcagaggcc ctctgatggt gacggggtcg gcggcgacca gctcgaacgg cgtcttggct
     2701 ttcatcg
//
LOCUS       DASZZC010000270         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_536040,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000270 DASZZC010000000
VERSION     DASZZC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536040"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VHE37_05750"
     CDS             <1..207
                     /locus_tag="VHE37_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35064.1"
                     /translation="RIGEAFARTAELIESSGAEGFRPMLAEARARWSTDAAERRQHLR
                     DARAGFERMRAPGHVARLDRLLAS"
     gene            214..>2258
                     /locus_tag="VHE37_05755"
     CDS             214..>2258
                     /locus_tag="VHE37_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT35065.1"
                     /translation="MRVCAQCTTKNRESARYCDQCGTRFEGAPAPAETASYTPRHLAE
                     QILNSRAAMVGERKQVTVLFADIKGSTRLAEAVGAEGWHEILNQFFALLAAAVHRYEG
                     TVNQYTGDGIMALFGAPIAHEDHAQRAAMAALEMQREVRRYAARLMVERQIEFGMRVG
                     LNTGEVVVGRIGDDLRMDYTAKGLTVNLAARMEQICEPGMINLSRYTAEQLRDEFLLR
                     DLGEREVAGALHPIRVYELQEARPQARLAAADGTAAPMFGRASELEQLKLALAQVRAG
                     EGRIVAISGEAGIGKSRLAREFLKACWQESVPVHHAAGVPYASAMPLAPVQVLLRSRL
                     ALAERATSDDVCARVASAFAPRSRRKSLVVPAITDFLGAGGGQERGAAVRSLMLERLA
                     RFLPRADTPQVLLIEDLHYADDATEDFLTRLCEQLAGSATLLLLTYRNEYASGWVAPY
                     LDQRIELAPLPADQVDQLARAQLGAAPELAAVARGIVERAGGNPFFVEEAVAALADEG
                     HLSGKPGDYRLARAIDEWPIPGSVQALVAGRVDRLPEQEKNLLQLAAVLGRVFDPALL
                     AACAGETAERDLASLAAAGFVRARAGGGYEFCHALTQEVTYHAQLDAQRSGTHGLAAA
                     ALEQRHGDAYVPAEAALQIAHHWREAQAWAKAGYWSMRAAEYFSARDARATLQHYRQA
                     "
BASE COUNT          327 a          807 c          833 g          291 t
ORIGIN      
        1 cgcatcggcg aggcgttcgc ccgcaccgcg gagctgatcg agtccagcgg cgccgagggt
       61 ttccgcccga tgctggcgga agcgcgcgcg cgctggagca cggacgccgc agagcgcagg
      121 cagcacctgc gcgacgcccg cgccggcttc gagcgcatgc gtgcgccggg ccacgtggcg
      181 cggttggacc ggctgctcgc gtcctaggca cgcatgcgcg tttgcgcaca gtgcaccacc
      241 aagaaccgcg agagcgcgcg ctactgcgac cagtgcggca cgcgctttga aggcgcgccg
      301 gcgccggccg aaaccgccag ctacacgccg cgccacctgg ccgagcagat cctcaactcg
      361 cgcgccgcca tggtcggcga acgcaagcag gtgacggtgc tgttcgccga catcaagggc
      421 tccacgcggc tggccgaagc ggtgggcgcg gaaggctggc acgagatcct caaccagttc
      481 ttcgcgctgc tggcggcggc ggtgcaccgc tacgagggca cggtgaacca gtacaccggc
      541 gacggcatca tggcgctgtt cggtgcgccg atcgcgcacg aggaccacgc gcagcgcgcg
      601 gccatggcgg cgctggagat gcagcgcgag gtgcggcgct acgccgcacg gctgatggtg
      661 gaacggcaga tcgagttcgg catgcgcgtg ggcctcaaca ccggcgaggt ggtggtcggc
      721 cgcatcggcg acgacctgcg catggactac accgccaagg gcctcactgt gaacctggcc
      781 gcgcgcatgg agcagatctg cgagccgggc atgatcaacc tgtcgcgcta caccgccgag
      841 cagctgcgcg acgagttcct gctgcgcgac ctgggcgagc gcgaagtggc cggcgcgctg
      901 cacccgatcc gcgtctacga gctgcaggaa gcgcggccgc aggcgcggct ggccgcggcc
      961 gatggaaccg cggcgccgat gttcggccgc gccagcgaac tcgagcaatt gaagctggcg
     1021 ctggcgcagg tgcgcgcggg cgagggacgc atcgtcgcca tcagcggcga ggcgggcatc
     1081 ggcaagtcgc gcctggcgcg cgagttcctc aaggcctgct ggcaggaatc ggtgccggtg
     1141 catcacgccg ccggcgtgcc gtacgcctcg gccatgccgc tggcgccggt gcaggtgctg
     1201 ctgcgctcgc gcctggcact ggccgagcgc gccacttccg acgatgtctg cgcgcgcgta
     1261 gccagtgcat tcgcaccgcg cagccggcgc aagagcctgg tagtgccggc catcaccgat
     1321 ttcctcggcg ccggcggcgg ccaggaacgc ggcgcggcgg tgcgctcgct gatgctggaa
     1381 cggctggcgc gcttcctgcc gcgcgccgac acgccgcagg tgctgctgat tgaagacctg
     1441 cattacgcag acgacgccac cgaagatttc ctcacgcgcc tgtgcgagca actggccggc
     1501 agcgccacgc tgctgttgct cacctaccgc aacgagtacg cctccggctg ggtggcgccg
     1561 tacctcgacc agcgcatcga gctcgcgccg ctgccggcgg accaggtgga ccagctggcg
     1621 cgcgcgcagc tcggcgccgc acccgaactg gcggcggtgg cgcgcggcat cgtcgagcgc
     1681 gccggcggca atccgttttt cgtcgaggaa gcggtggccg cgctggcgga tgaaggtcat
     1741 ctgtccggca agcccggcga ttaccgcctg gcgcgcgcca tcgacgagtg gccgatcccc
     1801 ggctcggtgc aggcgctggt ggcgggccgc gtggaccgtc tgcccgagca ggagaagaac
     1861 ctgctgcagc tggccgcggt gctcggccgc gtgttcgatc ccgcgctgct cgcggcctgc
     1921 gccggcgaga ccgccgagcg cgatctggca tcgctggccg ccgccggctt cgtgcgcgcg
     1981 cgcgccggcg gcggctacga gttctgccac gcgctgaccc aggaagtgac ctaccacgcg
     2041 cagctcgacg cgcagcgcag cggcacccac gggctggcgg cggccgcact cgagcagcgc
     2101 cacggcgacg cctacgtgcc ggccgaggcg gcgctgcaga tcgcgcacca ctggcgcgag
     2161 gcgcaggcct gggccaaggc cggctactgg agcatgcgcg ccgccgaata tttttccgcg
     2221 cgcgatgccc gcgccacgct gcagcactac cggcaggc
//
LOCUS       DASZZC010000271         8909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_275742,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000271 DASZZC010000000
VERSION     DASZZC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275742"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VHE37_05760"
     CDS             complement(<1..703)
                     /locus_tag="VHE37_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT35066.1"
                     /translation="MYRFLAAALAAFAAIGLMACHRSLPAHDTVSPIKHLVVIFQENV
                     SFDHYFGTYPLAANNSGEPAFTAAPNTPVGNTLITPLDPTHGFAPLAGVDLMNSNPNF
                     TNKANDDSSKNSQAQNPFRLSRAQAATMDQDHDYTPEQQAFDNGAMDLFPLYTGAGDT
                     AAPTSTTPPMNTTGLVMGYYDGNTVTALWNYAQHFAMSDNSFETQFGPSTVGALNLVS
                     GQTHGVVASNAAAGTS"
     gene            complement(738..3206)
                     /locus_tag="VHE37_05765"
     CDS             complement(738..3206)
                     /locus_tag="VHE37_05765"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HVT35067.1"
                     /translation="MKLLRIPTLALAGTLMAGCDHVPGAITGTADAPASLPAAPSPPA
                     APSGNWLRGDTHVHDDHSSDGSFPRQGISQSEPGNLSVAEQIGAGVRNGAQFLPLTDH
                     RTYDQHYDPLWESSQLLLIPGEEANGSPHATVLGAVDSIVQGGERPVGPGFIRLQQSI
                     WDAHAQDASWGTAHPDDGETNDDGSPNANANAQGVDTIEVWNRASTPDAEIDYAENRW
                     NAGFRTGIAGACDDHFKELWLIDGPGTPVTQVFAAQASERGVLDGLRSGRTRIHAQQP
                     LSPDVQLEADMQGDDVFEAIAGDEVIAPAGSKGRLRVTIANGAGTTVTVYKSPGRAAG
                     AWASFQPLSVLTEQTQLFDIETDGSDQWYRVEVRGVGQPAGVDTSTLSDPVANPPSLQ
                     TVTDTLLAVTSPIYIGPAPAAARPAVPIPADSGNEDGAALVYGSTGMFAGFPDLALSG
                     PHQHLVVEMHDAAGTHVLYRYAGGGGAAIDLAPASTTARFPKIAALGNEVWVAWQDTR
                     AGETPRRPAIYLRHSGDAGQSWDAEQLVRSLDGRAEHPAIAITRDGHPVLAWQEISAG
                     NPFDVMAQVVGVDAQPVNVSRDGKVIMAATTTDTRSARYPASVWPALAVAPDGSVALA
                     WQDDRTDADPLWTGGTGYGDGTDPDNWQIMVATRAAGTGTWATPASIGADDRADRHPS
                     IAYAADGTLAAAWDSKALQSSGANLEVLAAQSHDGGKTWSAPTAIAPDAIGMGQYPRL
                     GTDGNGAIRAAWYDSRSADWRWRIVTAVLAGDAWTHAQVIASRGNNTWPVINGGLIAF
                     AGTRNARRWQRDRTQQIFILRPPG"
     gene            complement(3580..5097)
                     /locus_tag="VHE37_05770"
     CDS             complement(3580..5097)
                     /locus_tag="VHE37_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVT35068.1"
                     /translation="MTFKFKPAVLAVVASTAASFAANAASPTLYGGGATFPAIAYVGP
                     SWESQSPQARLSKPADSGSLFGAYTARTGGAPAAGFPMVSYCQTGSGAGVKVLNGVLL
                     ATGSCSDYGTGSPAPAGFSGANSEPDFVASDSPLTSTDYNTFMGAKHAAHNEPVQMPS
                     VAGTVAIVYRNDSITAAKINLTESQICSIFSGAVTNWHQLSTAYKSKQIKVIFRRDSS
                     GTSFGFTNHLSSVCPTAEPVAQVDVFNTTKQFLPDNSTTGDGEQPTPGATTSGTAFLA
                     NAVPASPAVFLGYSGNGGVVNGVAATDGAIGYAEAADVIARENAGGASVKHATVSLKP
                     DVDSTHKYSKYDPISKAVTYALPSGSVLTDEIISGNDSHGRPVLKMITDATSGVSSGN
                     YPSQADCVVIVDPSGYATAATHVNGAGLTDYVGYPINIVTYLMTYNTGNGADASNVYN
                     LMYAPYYNGVQADSSKFRSSVTTIGANTGFAYLAGVTLPSPNTTAQTHLKACILK"
     gene            complement(5242..>8909)
                     /locus_tag="VHE37_05775"
     CDS             complement(5242..>8909)
                     /locus_tag="VHE37_05775"
                     /inference="COORDINATES: protein motif:HMM:NF023959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="filamentous hemagglutinin family protein"
                     /protein_id="HVT35069.1"
                     /translation="ERAASGTITLGSTARIDVSGGIGGGTVHVRAPLLVDHDVAVQFG
                     PGEQITGARDVTVEAYATWSTLDATTGAQHFDGVIDPAGWYSAAGTQLPGTTVAGFFT
                     PASANASHVGFYGDTLGNFVRAPFGFESRFAGIQNLSIRAGIDLLNPSGSVNQGDIAV
                     LSNWNLGAGQGAAGTASFSYRHDGAAPVLSLRAVHDINFSASLSDGFFQYSNPLGTLA
                     TDNSASPVATAGNPLPLLTANLAGGDSSSYRIVAGAEAGSADPLALGGSDGSVTLNGH
                     TTAVFVKNAGGATADMVAPTMIRTGTGTIDIVAATDVSMTDPLAPGVIYTGGRPADGT
                     TAPTVTQVIKGKGNIPVLIDTGDVQPVDAGDLSISAGRDIIGIERVIDSSGRRSGRVG
                     TNLTQLWWPWMERSCVFTDTCGTAGAQQDSSIGFGIFDQGIMSVGGDITVAAGRNLQD
                     LSVSLPATWRITTGTSPAVTTYGGGDARISAGGNLYSGAYFAAAGRMSLAVGGSVSAE
                     LASASGAPIGTFFALQDGQLRLSAAGDINVAGLFDPSYLYTNFDSQGYSAASSFSATS
                     SAGSIAFNSLAVPVAEYGFGNSTLAPAYSAVLPATLELTALNGGITIARKGELYPSAQ
                     GNLELLAQDSIRLYQAQGGDTSNRYFGLIDAPASVLPSPLSPIGALLHSATTFLDRGG
                     DSALSLHTDDPLHAQDGQPVLVYSLEGDIIDGDANHRLPLTLFADKPAQIRAGRDVVD
                     LSLRGQNLYDSDITLIAAGRDIVDSPLTQSRQVPLIQLGGIGTLGLQAGRDIGPLTSA
                     VEALHDGLLPANNTPYPGIFTVGNLFNAHLDHAGANLAVLFGVGPGMTVDDFAAAYID
                     PAHADIGFGPENAALVQFVSTWQADQQRRSGQPASTAQLSADDAWQIFQTLPLLQRQR
                     FASMVLFDVLNQTGLDFNKPDSLYFQQYQRGYAAIERLFPAALGYTHNDLTGALNGAL
                     APVATGTFDMRGSTLQTQQGGDIAILGPGGSVLIGSAAAPPSVAGNSQNARIGPNNQG
                     ILTLERGNIDLFADRDVLLAQSRIFTEQGGDLVIWSSNGDINAGKGAKTTTEVPPAQY
                     FCDNDHYCRVDAKSQVSGAGIATLQSRPEDPAGNANLIAPHGTIDAGDAGIRSAGALN
                     VAALRVANADNIQVSGKTSGVPTGQVDTGALAAASSVSAAVEQSASQASLAKPADTAA
                     TLITVEVVGYGGDGTEE"
BASE COUNT         1378 a         2852 c         3134 g         1545 t
ORIGIN      
        1 cgctggtgcc ggcggccgca ttcgatgcga cgacgccatg ggtctgcccg gacaccaggt
       61 tgagcgcgcc gaccgtggaa ggtccgaact gcgtctcgaa cgagttgtcg ctcatggcga
      121 agtgctgggc gtagttccac agcgcggtca ccgtgttgcc gtcgtagtag cccatcacca
      181 ggccggtggt gttcatcggc ggcgtggtgc tggtcggcgc cgcggtgtcg ccggcgccgg
      241 tgtacagcgg aaacaggtcc atggcgccgt tgtcgaacgc ctgctgctcc ggcgtgtaat
      301 cgtggtcctg gtccatggtg gccgcctgcg cgcggctgag gcggaacggg ttctgcgcct
      361 gcgagttctt gctcgagtcg tcgttggcct tgttggtgaa gttggggttg ctgttcatca
      421 ggtccacgcc ggccagcggc gcaaagccat gcgtgggatc gagcggggtg atcagcgtgt
      481 tgcccaccgg cgtgttcggc gccgcggtga aggccggctc gccgctgttg ttcgccgcca
      541 gcggataggt gccgaagtag tgatcgaacg acacgttctc ctggaagatc accaccaggt
      601 gcttgatcgg cgaaacggtg tcgtgcgcgg gcagcgagcg atggcaggcc atcaggccga
      661 tggcggcgaa tgcggcgagt gctgcggcaa gaaatcggta catgctgccc ttcccttatt
      721 tgaaatgcgt gagtgcgtca gccgggcgga cgcaggatga aaatctgctg ggtgcggtca
      781 cgctgccagc gccgcgcgtt gcgcgtgccg gcaaacgcga tgagcccgcc gttgatgacc
      841 ggccaggtgt tgttgccgcg cgaggcaatc acctgcgcat gcgtccaggc gtcgcccgcg
      901 agcacggcgg tgacgatgcg ccagcgccag tcggccgagc gcgagtcgta ccaggcggcg
      961 cggatggcgc cattgccatc cgtgccgagg cgcgggtact ggcccatgcc gatcgcatcc
     1021 ggcgcgatgg ccgttggcgc gctccaggtc ttgccgccgt cgtgcgactg tgcggcgagc
     1081 acttcgagat tggcgccgga ggactgcagc gccttgctgt cccaggccgc ggcgagcgtg
     1141 ccatcggcgg cgtaggcgat cgatggatgg cggtcagcgc ggtcgtccgc accgatgctc
     1201 gccggagtcg cccacgtgcc ggttcccgcc gcgcgcgtcg ccaccatgat ctgccagttg
     1261 tcgggatcgg tgccgtcgcc atagccggtg ccgccggtcc acaacgggtc tgcatcggtg
     1321 cggtcatcct gccaggccaa ggccacgctg ccgtctggag ccaccgcgag cgccggccac
     1381 accgacgccg ggtagcgcgc actgcgcgtg tcggtggtgg tcgccgccat gatcaccttg
     1441 ccgtcgcgtg aaacattgac cggttgagcg tcgacgccga cgacctgcgc catcacgtcg
     1501 aaaggattgc ctgcgctgat ttcctgccag gccagcaccg ggtggccgtc gcgcgtgatg
     1561 gcaatggccg gatgctcggc gcggccgtcg agactgcgca ccagctgctc cgcatcccag
     1621 ctttggccgg cgtcgccgga atggcgcagg tagatggccg gccgccgcgg cgtttccccg
     1681 gcgcgcgtgt cctgccaggc cacccacacc tcgttcccca gcgcggcgat tttcgggaag
     1741 cgcgccgtag tgctggccgg cgccagatcg atcgccgcgc cgccgccacc tgcatagcgg
     1801 tacagcacat gcgtgccggc ggcatcgtgc atttccacga ccaggtgctg atgcgggccg
     1861 ctgagcgcga gatcgggaaa tccggcgaac atgccggtgc tgccgtagac cagcgccgcg
     1921 ccgtcttcgt tgccgctgtc cgccggaatc ggcaccgcgg gccgcgcggc tgccggggcc
     1981 gggccgatgt agatgggcga ggttaccgcc agcagcgtgt cggtgacggt ctgcagcgac
     2041 ggcggattcg ccaccggatc gctcagcgtg ctggtgtcga cgccggccgg ctgcccgaca
     2101 ccgcgcactt cgacgcggta ccactggtcg ctgccgtcgg tctcgatgtc gaacagctgc
     2161 gtctgctcgg taagaaccga cagtggctgg aagctggccc aggcgccggc agcacggcct
     2221 ggcgacttgt acacggtgac cgtggtgccg gcgccgttgg cgatggtgac gcgcaggcgg
     2281 cccttgctgc cggcgggcgc aatcacttcg tcgccggcga tggcttcgaa cacgtcgtcg
     2341 ccctgcatgt ccgcttccag ctgcacgtcg ggcgacagcg gctgctgcgc gtggatgcgc
     2401 gtgcgcccgc tgcgcagtcc gtcgagcacg ccgcgttcgc tggcctgcgc cgcgaacacc
     2461 tgcgtcaccg gcgtgccggg gccgtcgatc agccacagtt ccttgaaatg gtcgtcgcag
     2521 gcgccggcga tgccggtgcg gaagcccgcg ttccagcggt tttccgcgta gtcgatctcg
     2581 gcatccggcg tgctcgcgcg attccacacc tcgatggtgt cgacgccctg tgcattggca
     2641 ttggcgttgg gcgaaccgtc gtcgttggtc tcgccgtcgt cgggatgcgc cgtgccccag
     2701 ctggcatcct gcgcatgcgc gtcccagatg gactgctgca ggcggatgaa gccggggccc
     2761 accggccgct cgccgccctg cacgatgctg tcgaccgcgc cgagcacggt ggcatgcgga
     2821 ctgccgttgg cctcctcgcc cgggatcagc agcagctgcg acgattccca cagcggatcg
     2881 taatgctggt cgtaggtgcg gtggtcggtc agtggcagga actgcgcgcc gttgcgaacg
     2941 cccgcgccga tctgctccgc caccgacagg ttgccgggct cgctctgcga aatgccctgg
     3001 cgcggaaagc tgccgtctga ggaatggtcg tcgtgcacat gcgtgtcgcc gcgcaaccag
     3061 ttgcccgacg gcgccgccgg cggtgacggc gcagcgggca gcgacgcggg agcgtctgcc
     3121 gtgccggtga tggcgccggg cacgtggtcg catccagcca tcaaggtgcc ggccagtgcc
     3181 agcgtgggaa ttcgcaacag tttcatgatt ccgctccccg cgagagatgc gcacattctg
     3241 gcgcgtgact tgcgcgccat gcgtactgcg ggcgagagcc gcattgtggt cagcgattgt
     3301 tgacgttgcg tgacaggcgg ctgggagaat ctgctcactg cggcgccggt gcgcgggtaa
     3361 aacggtcaac cgcactgcca tccggcttgt tatttaatat cttatatttt tttaatttat
     3421 taatatttta taacacccca aaacgccgtg cggttggctc atgcaagtgc ccggatgccg
     3481 attttgcgtc gcccataaaa aatccccgcc tcttgcgaag cggggacagg agagaccgcg
     3541 aaagctccgc cgcgaacggc ggcgatcacg ggttgttgtt tacttcagga tgcaggcctt
     3601 caggtgcgtc tgcgccgtgg tgttcggcga aggcagcgtc acgcccgcca gataggcgaa
     3661 gccggtgttg gcaccgatcg tggtcacgct gctgcggaac ttgctgctgt ccgcctgcac
     3721 gccgttgtag tacggggcat acatcaggtt gtagacgttg ctggcatccg caccgttgcc
     3781 cgtgttgtag gtcatcaggt aggtgacgat gttgatcgga tagccgacgt agtcggtgag
     3841 gccagcgccg ttcacgtggg tggcagccgt ggcgtagccg gacggatcga cgatgaccac
     3901 gcagtccgcc tgcgacgggt agttgcccga gctcacgccc gaagtcgcgt cggtgatcat
     3961 cttcagcacc ggacggccgt ggctgtcgtt gccgctgatg atttcatcgg tcagcaccga
     4021 accggacggc agcgcgtagg tgacggcctt ggagatcgga tcgtacttgg agtacttgtg
     4081 cgtggaatcg acatccggtt tgagcgacac ggtggcgtgc ttgacgctgg cgccgccggc
     4141 gttttcgcgc gcgatcacgt cggccgcttc ggcatagccg atggcaccgt cggtcgcggc
     4201 aacgccgttg accacaccgc cgttgccgga atagccgagg aacacggccg gcgatgccgg
     4261 aaccgcgttc gccaggaaag ccgtacccga cgtggtcgca cccggagtcg gctgctcgcc
     4321 atcaccggtt gtgctgttgt ccggcaggaa ctgcttggtg gtgttgaaca cgtccacctg
     4381 ggccaccggc tcagccgtgg ggcagaccga cgacaggtgg ttggtgaagc cgaacgaggt
     4441 gccgctgctg tcgcggcgga agatcacctt gatctgcttg ctcttgtagg cggtcgacag
     4501 ctggtgccag ttggtcaccg cgccggagaa gatgctgcag atctgcgatt cggtcaggtt
     4561 gatcttcgca gcggtgatcg agtcattgcg atacacgatg gccacggtgc cggccaccga
     4621 aggcatctgc acgggctcat tgtgagcggc gtgcttggcg cccatgaagg tgttgtagtc
     4681 ggtcgaagtc agcggcgaat ccgaggcgac gaaatcaggc tcggagttgg cgccggagaa
     4741 accggcagga gccggtgagc ccgtgccgta gtcgctgcag ctgcccgtgg cgagcagcac
     4801 gccgttgagc accttgacgc cagcgcccga accggtctgg caatacgaga ccatggggaa
     4861 gccggcagcc ggcgcgccgc cggtgcgcgc ggtgtaagcg ccgaacagcg agccgctatc
     4921 tgccggtttg gacaggcgcg cctgcggcga ctggctttcc cacgacgggc ccacgtatgc
     4981 gattgcaggg aaggttgcgc caccgccgta cagcgtcggc gaggcagcgt tggcggcaaa
     5041 gctcgccgcc gtcgaggcaa ccacggccag gacggccggc ttgaacttga aggtcatgaa
     5101 ttactcctgg agaaaccgtt tatgtcgggc gcccggcacc gtgatctagc gtggagctcc
     5161 ccctcagggc gatccgagcg cggcgacgat accggtcgaa tcttgcagtt ctctgacggt
     5221 ttcaagacaa aagcggcttg gctactcctc ggtgccgtcc ccgccatagc cgaccacttc
     5281 caccgtgatc agcgttgccg ccgtgtcggc cggcttggcc aggctggcct ggctggccga
     5341 ctgctcgacg gccgcggaca cggaactggc cgcggccagt gcgccggtgt cgacctggcc
     5401 ggtcggcacg ccggaggtct tgcccgacac ctggatgttg tcagcgttgg ccacgcgcag
     5461 cgccgccacg ttgagcgcgc cggccgagcg gatgccggcg tcgccggcgt cgatggtgcc
     5521 gtgcggcgcg atcaggttgg cattgcccgc cggatcttcc gggcgcgact gcagcgtggc
     5581 gatgccggcg ccgctgacct gcgacttggc gtcgacacgg cagtaatgat cgttgtcgca
     5641 gaaatactgc gccggcggca cctcggtggt ggtcttcgcg cccttgccgg cgttgatgtc
     5701 gccgttggaa ctccagatca ccaggtcgcc gccctgctcg gtgaagatgc ggctctgcgc
     5761 cagcagcacg tcgcgatcgg caaacaggtc gatgttgccg cgctcgagcg tgaggatgcc
     5821 ctggttgttc ggcccgatgc gggcattctg cgaattgccc gcgacgctcg gcggcgccgc
     5881 ggcactgccg atcagcacgc tgccgccggg ccccaggatg gcgatatcgc ctccctgctg
     5941 cgtctgcagc gtcgagccgc gcatgtcgaa ggtgccggtg gccaccggcg ccagcgctcc
     6001 gttgagcgcg ccggtgagat cgttgtgcgt atagccgagc gctgcgggga acaggcgctc
     6061 gatcgccgca tagccgcgct ggtactgctg gaaatagagg ctgtccggct tgttgaaatc
     6121 caggccggtc tggttgagca catcgaacag caccatcgag gcgaagcgct ggcgctgcag
     6181 cagcggcagg gtctggaaga tctgccacgc gtcgtccgcg ctcagctgcg cggtgctggc
     6241 gggctggccg ctgcgccgct gctggtccgc ctgccaggtg gagacgaact gcaccagcgc
     6301 cgcattctcc gggccaaagc cgatgtcggc atgtgccggg tctatgtagg ccgcggcgaa
     6361 atcatccacc gtcatgccgg gcccgacacc gaacagcacg gcgaggttgg cgccggcgtg
     6421 atcgagatgc gcgttgaaca ggttgccgac ggtgaagatg cccgggtacg gcgtattgtt
     6481 ggccggcagc aacccgtcgt gcagtgcttc gaccgcggag gtcagcgggc cgatgtcgcg
     6541 gccggcctgc aacccgagcg tgccgatgcc gcccagctgg atcagcggca cctggcgcga
     6601 ctgcgtcagc ggcgaatcga cgatatcgcg gcccgccgcg atgagggtga tgtcggaatc
     6661 gtagaggttc tgtccgcgca ggctgagatc gaccacatcg cgcccggcgc ggatctgcgc
     6721 cggcttgtcg gcgaacaggg tcagaggcag gcggtgattg gcatcgccgt cgatgatgtc
     6781 gccttcgagg ctgtacacca gcaccggctg accgtcctgc gcgtggagcg gatcgtcggt
     6841 gtgcaggctc agggccgaat cgccgccgcg atcgaggaac gtggtggcag agtgcagcaa
     6901 cgcgccgatg ggcgacagcg gcgacggcag cacgctggcg ggtgcatcga tcaggccgaa
     6961 atagcggttg ctggtgtcgc cgccctgcgc ctggtacagg cggatggaat cctgcgccag
     7021 cagttcgagg ttgccctgcg ccgacggata cagctcgccc ttgcgcgcga tggtgatgcc
     7081 gccgttgagc gcggtgagtt ccagcgtggc cggcagcacg gcgctgtacg ccggcgccag
     7141 cgtgctgttg ccaaacccat actccgccac cggcacggcc agcgaattga aggcaatgct
     7201 gccggcgctg ctggtcgcgc tgaagctcga cgcggccgaa taaccttgcg aatcgaagtt
     7261 cgtgtacagg tacgaaggat caaacagccc ggcgacgttg atgtcgcccg cggccgacag
     7321 ccgcagctgg ccatcctgca gcgcgaagaa cgtaccgatg ggagcgcctg aagcactcgc
     7381 aagctcggcg gagacgctgc cacccacagc cagtgacatg cggccggccg cggcgaagta
     7441 cgcgccgctg tacaggttgc cgccggcgct gatccgcgca tcgccgccgc cataggtcgt
     7501 caccgcgggc gacgtgccgg tggtgatgcg ccaggtggca ggcagggaca ccgaaagatc
     7561 ctgcaggttg cgaccggcag caacggtgat gtcgccgccc acgctcatga tgccctgatc
     7621 aaagatgccg aagccgatcg aggaatcctg ctgcgcaccg gcagtgccgc aggtgtcggt
     7681 gaacacgcag ctgcgttcca tccagggcca ccacagttgc gtgaggttgg tgcccacgcg
     7741 cccgctgcgg cggccgctgc tgtcgatgac gcgttcgatg ccaatgatgt cgcggccggc
     7801 actgatggaa agatcgccgg catccactgg ctggacatcg ccggtatcga tcagcacggg
     7861 aatatttccc ttgcccttga tcacctgcgt caccgtcggt gcggtggtgc cgtcagccgg
     7921 acggccgccg gtgtagatca ctccgggcgc cagcggatcg gtcatcgaca catcggtcgc
     7981 ggcaacaatg tcgatcgttc cggtgccggt tcggatcatg gtcggcgcga ccatgtcggc
     8041 cgttgctccg ccggcattct tcacgaagac ggccgtggtg tggccgttga gcgtgacgct
     8101 gccgtcgctg ccacccagcg ccagcggatc ggcactgccc gcttccgcgc cggcgacgat
     8161 gcggtaggaa gacgagtcgc cgccggcaag attggccgtg agcaatggca ggggattgcc
     8221 ggccgtcgca accggcgaag cgctgttgtc ggtggccagc gtacccagcg gattggagta
     8281 ctggaagaaa ccatcgctga gactggcgga gaaattgatg tcgtgcaccg cacgcaggct
     8341 cagcaccggc gctgcgccgt cgtgccggta actgaacgaa gcggtgcccg cggcgccctg
     8401 gccggcgccg agattccagt tgctcagtac tgcgatgtcg ccctggttca cgctgccgga
     8461 cggattgagc aggtcaatgc cggcgcggat gctcaggttc tggatgccgg caaagcggct
     8521 ttcaaaacca aacggcgctc gcacgaaatt gcccagggtg tcgccataga agccgacgtg
     8581 gctggcattg gccgaggccg gcgtgaagaa tcctgcaacc gtcgtccccg gcagctgggt
     8641 gccggccgcg ctgtaccagc ccgccggatc gatcacgccg tcgaagtgct gcgcgccggt
     8701 ggtcgcatcg agtgtgctcc aggtggcgta ggcctcgacc gtcacatcgc gcgcgccggt
     8761 gatctgctcg cccggcccga actgcaccgc cacgtcgtga tcaaccagca gcggcgcgcg
     8821 gacatgcacg gtaccgccgc cgatgccgcc actgacgtcg atgcgcgcag tgctgccaag
     8881 agtgatggtg ccggaggctg cacgctcta
//
LOCUS       DASZZC010000272         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_663924,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000272 DASZZC010000000
VERSION     DASZZC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663924"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2399
                     /gene="mfd"
                     /locus_tag="VHE37_05780"
     CDS             <1..2399
                     /gene="mfd"
                     /locus_tag="VHE37_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HVT35070.1"
                     /translation="SAAPPRGADEIKAYLASTPDRVLFIAESAGRREALLAWLKPLGI
                     QAREYADWAQFLTDNKRYGIALGPLQDNLRLPGENISIIAEAAVFGLHAPQQQRRRTK
                     VRDPETLLRDLGALMPGSAVVHVQHGVGRYLGLQRMEAGGAEAEFLVLEYSGGDKLYV
                     PVASLQLVHRYTGSEEAAAPLHQLGSERWAKAQAKAREKAHDVAAELLQIQARRMAKA
                     GQPVTVDAADYEAFARGFPFAPTVDQQKAIDAVLADLQAEKIMDRVVCGDVGFGKTEV
                     ALRAAYAVARAGKQVCVLAPTTLLVEQHAKNFSDRFAYMNSGAESSARPIRIGALSRM
                     RTAREQAAMLKQLEDGTLDIVIGTHRLLQGDVKFRNLGLVVVDEEHRFGVRHKERLKN
                     LRAEVDLLTLTATPIPRTLNMSLAGLRDLSIIATPPESRLAIKTFVAEWSNALVVEAC
                     QRELRRGGQVYVLHNEVADIAVFAQKMAELVPEGSVRFAHGQMRERELEQVMLDFYHQ
                     RFNILVCTTIIESGIDVPTANTILIDRADSLGLAQLHQLRGRVGRSHHRAYAYLLVPS
                     KRAITTDAQKRLDAIEQAGELGAGFALATHDLEIRGAGELLGEEQSGQIEEVGFTMYA
                     DLLARAVKAIKSGKLDDTPFGQAECEVDLGVSALIPEDYVPDVHTRLTLYKRLAECRE
                     ERQLDEFRVELIDRFGLLPPQLERLCEAAQLRIHACALGIARVRAGAMSVSLEFSARP
                     TIDPLAIIKLIQREPKKYKLEGQKRLHHYASMEAAEQRAATVQMLLDTLAGKTSPVKK
                     "
BASE COUNT          434 a          837 c          825 g          357 t
ORIGIN      
        1 cgtccgcagc gccgccgcgc ggcgccgatg agatcaaggc ttatctcgcg tccaccccgg
       61 accgcgtgct gttcatcgcc gaatccgccg gccgccgcga agcgctgctg gcctggctga
      121 aaccgctggg catccaggcg cgcgaatacg cggactgggc gcagttcctc accgacaaca
      181 agcgctacgg catcgcgctc ggtccgctgc aggacaacct gcgcctgccg ggcgagaaca
      241 tttccatcat cgccgaggcc gcggtgttcg gactgcacgc gccgcagcag cagcgccgcc
      301 gcaccaaggt gcgcgatccg gaaaccttgc tgcgcgatct gggcgcgctg atgccgggtt
      361 cggcggtggt ccacgtgcag cacggcgtcg gccgctatct gggcctgcag cgcatggagg
      421 ccggcggcgc cgaggccgag ttcctggtgc tggaatattc cggcggcgac aagctgtacg
      481 tgccggtggc ctcgctgcag ctggtgcacc gctacaccgg ctcggaggaa gccgccgcgc
      541 cgctgcacca gctgggcagc gagcgctggg ccaaggccca ggccaaggcg cgcgagaaag
      601 cgcacgacgt cgccgccgag ctgctgcaga tccaggcgcg gcgcatggcc aaggccggcc
      661 agccggttac cgtcgatgcg gccgactatg aagcgttcgc ccgcggcttt ccgtttgcgc
      721 cgacggtgga ccagcagaaa gccatcgacg cggtactcgc cgacctgcag gccgagaaaa
      781 tcatggaccg cgtggtctgc ggcgacgtcg gcttcggcaa gaccgaagtc gcgctgcgcg
      841 ccgcgtatgc cgtcgcgcgc gccggcaagc aggtgtgcgt gctggcgcca accacgctgc
      901 tggtcgagca gcacgccaag aatttctccg accgcttcgc ctacatgaat tcaggcgccg
      961 aatcatcggc gcggccgatc cgcatcggcg cgctgtcgcg catgcgcacg gccagggagc
     1021 aggccgccat gctcaagcag ctggaagacg gcacgctcga catcgtcatc ggcacgcatc
     1081 gcctgctgca gggcgacgtc aagttcagga acctgggact ggtggtggtc gacgaggagc
     1141 accgcttcgg cgtgcgccac aaggagcgct tgaagaacct gcgcgccgaa gtggacctgc
     1201 tcacgctcac cgccacgccg atcccgcgca cgctcaacat gagcctggcc ggcctgcgcg
     1261 atctttcgat catcgccacg ccgccggagt cgcgcctggc gatcaagacc ttcgtcgccg
     1321 aatggagcaa cgcgctggtg gtcgaggcct gccagcgcga actgcgccgc ggcggccagg
     1381 tttacgtgct gcacaacgaa gtggccgaca tcgccgtgtt cgcgcagaag atggcggagc
     1441 tggtgccgga aggcagcgtg cgctttgccc atggccagat gcgcgagcgc gaactcgagc
     1501 aggtgatgct cgacttctac caccagcgct tcaacatcct ggtctgtacg acgatcatcg
     1561 aatcgggcat cgacgtgccc acggcgaaca ctatcctgat cgatcgcgcc gactcgctcg
     1621 ggctggcgca gctgcaccag ctgcgtgggc gcgtcggccg ctcgcaccat cgcgcctatg
     1681 cctacctgct ggtgccgagc aagcgcgcca tcaccaccga cgcacagaag cggctcgacg
     1741 ccatcgagca ggcgggcgaa ctcggcgccg gcttcgcgct ggccacgcac gacctggaaa
     1801 tccgcggcgc cggcgaactg ctgggcgagg aacaatcggg gcagatcgag gaagtcggat
     1861 tcaccatgta cgccgacctg ctggcgcgcg cggtcaaggc gatcaagagc ggcaagctcg
     1921 acgacacccc gttcggccag gccgagtgcg aagtcgacct cggcgtgtcg gcgctgatcc
     1981 cggaagatta cgtgcccgac gtgcacacgc gcctgaccct gtacaagcgc ctggccgagt
     2041 gccgcgagga gcgacagctc gacgagttca gggtcgaact gatcgaccgc ttcggtctgc
     2101 tgccgccgca gctcgagcgc ctgtgcgagg cggcgcagct gcgcatccac gcctgcgcgc
     2161 tgggcattgc gcgcgtgcgc gccggcgcca tgagcgtgtc gctggagttc agcgcaagac
     2221 cgacgatcga tccgctggcg atcattaaat tgatccagcg cgagccgaaa aaatacaagc
     2281 tcgaaggcca gaagcgcctg catcactacg ccagcatgga agcggcggag cagcgcgcgg
     2341 ccacggtgca gatgttgttg gatacactgg ccggcaaaac cagcccggtc aaaaaatgaa
     2401 gcgcacacgt gccttgctgc tgctgatgct gtgcctgttg ccgccgacgg cgc
//
LOCUS       DASZZC010000273         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_275958,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000273 DASZZC010000000
VERSION     DASZZC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275958"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VHE37_05785"
     CDS             <1..182
                     /locus_tag="VHE37_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT35071.1"
                     /translation="AAFIRNSERIKKTPIIMITSRSGDKHRERARQIGVNRYMIKPFQ
                     EDQLMAEIREVMVPA"
     gene            179..661
                     /locus_tag="VHE37_05790"
     CDS             179..661
                     /locus_tag="VHE37_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVT35072.1"
                     /translation="MSAVSQIYAVLMALEGDSILLPNVAIAEVVQRERVQAPEPGAPE
                     WFAGYLDWHSRRVPVISFELLNGGRTSGRSRRDRVAIVNSLGVHLPSGAFGVITQGYP
                     HLVTLNRTAVRPMALRETDRPGLLSARVKIASQEALIPDLATIEADIQRTTSVLSAAA
                     "
     gene            complement(674..2767)
                     /locus_tag="VHE37_05795"
     CDS             complement(674..2767)
                     /locus_tag="VHE37_05795"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HVT35073.1"
                     /translation="MLLSMAGLGLAACSGGAPSADTSSSDPLSTPKSRLALKAFAPDC
                     SDFLSTTADQLTEQYLDWFRCPPGAMCPIAIDGPLTPGTAGAPAGAGAPLRVSQTNTQ
                     ETGVDEPDIVRSDSQGNIYVLRNSSLLAFAGVPASSLGSNPTSTLALGDENFYATDFF
                     LDEAAQRLVIFGQQYDSNTSKAVTLFASTGDPLNPALVGSVAIEGYGIDARRIGGRVH
                     RVSGFYPNLPSWFYDNSDPLEQLREQYRKAQANNDQTTADQLHDQVRTEIGKRVDDAG
                     ATAFLPVVSSNLGGTDSSGYLACDQLSHPDVTTGEGMTLVDSFDTTGDHRDAADITSY
                     NFLIYASTQNLYLVQPSFGWFFAPLQRDETAVFRLALSDSGPARYSGLGKVQGWVRDS
                     YALSEFDNMLRVVTSTYQQSSSNSDMLEPVTQLAVLDATQADMPKVGGIDAIAAGEQL
                     QGARFFGTRGFVDTFRYIDPLFALDLSDPTHPVVADELSMPGFSSYIAPVGDNLLLTV
                     GRGGTDQRLNGQVGVGLFDIADLHQVKQLASLSPSAGDSSYSYSTAEYDPHAFSYFAD
                     SADTPSPGTLSIPLQTYSSDTPADDFSGFLVVRVDAAADAPLAELGRVDHRGLGDAGG
                     CAYPGATAGGGTAGGGTTTARPCYNGYYTQPRRSIFMQQTDGISLYTFSDLGVIASDA
                     AAPDTEQGRKVFPAP"
     gene            2848..>3027
                     /locus_tag="VHE37_05800"
     CDS             2848..>3027
                     /locus_tag="VHE37_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT35074.1"
                     /translation="MAQQADCVVIGAGHNGLVASAYLARAGRRVIVLERHDTVGGAAV
                     TEEIAPGYRSSTASYV"
BASE COUNT          517 a          965 c         1022 g          523 t
ORIGIN      
        1 tggctgcgtt catccgcaac agcgagcgca tcaagaagac gccgatcatc atgatcacct
       61 cgcgttcggg cgacaagcac cgcgagcgcg cccgccagat cggcgtcaac cgctacatga
      121 tcaagccgtt ccaggaagac cagttgatgg ctgaaatccg cgaagtgatg gtgccggcat
      181 gagcgccgta tcgcagatct acgccgtgct gatggcattg gaaggcgaca gcatcctgct
      241 gcccaacgtg gccatcgccg aggtggtgca gcgcgagcgg gtgcaggcgc ccgagcccgg
      301 tgcgccggaa tggtttgccg gctatctcga ctggcactcg cggcgtgtgc cggtgatcag
      361 cttcgaactg ctcaacggcg gccgcaccag cggccgttcg cggcgcgatc gggtggctat
      421 cgtcaattca cttggcgtgc acctgcccag cggcgctttc ggcgtcatca cccaggggta
      481 tccgcacctg gtcacgctca accgtacggc cgtgcggccg atggcgctgc gcgaaaccga
      541 tcgcccggga ttgctctcgg cgcgggtcaa gattgccagc caggaagcgc tgattcctga
      601 cctggcgact atcgaggccg acatccagcg caccaccagc gtgctcagcg ccgcggcctg
      661 acgcggttgc gcctcagggc gcagggaaca ccttgcggcc ctgttcggta tccggtgcgg
      721 cagcatcgct cgcaatcacg ccgagatcgg aaaacgtgta cagcgaaatc ccgtcggtct
      781 gctgcatgaa gatgctgcgc cgcggctggg tgtagtaacc gttgtagcag gggcgcgcgg
      841 tcgtcgtgcc gccgcccgct gtgccgccgc cggcggtggc tccgggatag gcgcagcctc
      901 cagcgtcccc cagcccgcga tgatccaccc ggcccagttc cgccagcggc gcatcggcag
      961 ccgcatcgac ccgcaccacc aggaagccgg agaagtcgtc cgccggcgtg tcgctgctgt
     1021 aggtctgcag cgggatgctc aaggtgccgg ggctgggcgt atcggcgctg tcggcgaaat
     1081 agctgaaggc atgagggtca tattccgcgg tgctgtagct gtaactgctg tcgccggcgg
     1141 aaggcgacag cgacgccagc tgcttcacct gatgcaggtc ggcaatgtcg aacagcccga
     1201 cgccaacctg tccattcagg cgctggtcgg ttccgcctcg gcccaccgtc agcagcaggt
     1261 tgtcgccgac cggagcgata tagctggaga agcccggcat gctcaattca tcggcaacga
     1321 ccggatgggt cgggtccgac aggtccaggg cgaacagcgg gtcgatgtaa cggaaggtgt
     1381 cgacgaatcc acgcgtgccg aagaagcgcg cgccttgcag ctgttcgccc gcggcgatgg
     1441 cgtcgatgcc gcccaccttc ggcatgtcgg cctgggtggc atcgagcacg gccagttgcg
     1501 tgacgggctc gagcatgtcg ctgttgctgg aactctgctg gtaggtgctg gtgaccacgc
     1561 gcagcatgtt gtcgaattcc gacagggcat agctgtcacg cacccatccc tgcaccttgc
     1621 ccagtcccga gtacctagcc ggcccgctgt ccgacagcgc caggcggaac acggcggttt
     1681 cgtcgcgctg cagaggtgcg aagaaccagc cgaagctcgg ctgcaccagg tagagattct
     1741 gcgtcgaagc gtagatcagg aagttgtagc tggtgatgtc ggctgcgtcg cgatggtcgc
     1801 cagtggtatc gaagctgtcg accagcgtca tgccttcgcc ggtggtcaca tcgggatgcg
     1861 acagctggtc gcaggccaga tagccgctgc tgtcggtgcc gccgaggttg ctgctcacca
     1921 ccggcaggaa ggccgttgcg ccggcatcgt cgacacgctt gccgatctcg gtgcggactt
     1981 ggtcgtgcag ctgatcggcc gtggtctggt cattgttggc ttgcgccttc cggtactgtt
     2041 cgcgcagctg ctccagcgga tcactgttgt cgtagaacca ggaaggcagg ttgggataaa
     2101 agccgctgac gcggtgcacg cggcctccga tgcgccgcgc gtcgatgcca tagccttcga
     2161 tggcaacgct gccgaccagc gccggattga gcgggtcgcc cgtgctggcg aacagcgtca
     2221 ccgccttcga ggtattgctg tcgtactgct ggccgaagat caccaggcgt tgcgccgcct
     2281 cgtcgaggaa gaagtcggtc gcatagaaat tctcgtcgcc taatgccagc gtgctggtgg
     2341 ggttgctgcc caggctgctg gccggcacgc ccgcgaaggc aagcaggctg ctgttccgca
     2401 gcacatagat gttgccctgg ctgtcgctgc gcacgatatc cggctcgtca accccggttt
     2461 cctgggtgtt ggtctgcgac acgcgcagag gcgcgccggc accggcgggt gcgccggcgg
     2521 tgcccggtgt cagcggcccg tcgatggcga tcgggcacat cgcgcctggc gggcagcgga
     2581 accagtcaag gtactgttcg gtgagctggt cggcagtggt ggaaagaaaa tcgctgcagt
     2641 ccggcgcaaa cgccttcaat gccagccggc tcttcggcgt gctcagcgga tcgctggaac
     2701 tggtgtccgc cgaaggcgcg ccgccgctgc aggcagcaag gccgagccca gccatggaca
     2761 gcagcataaa tgcgccagcg cgacgatggg ttggagataa catagccggc ctcccggaaa
     2821 cgcaacagct aacctactcc cgacgccatg gcccagcaag cggattgcgt cgtcatcggc
     2881 gcaggacata acggactagt cgcctctgca tatctggcgc gcgccggcag gcgcgtgatc
     2941 gtgctggaac ggcacgacac cgtcggcggc gccgccgtga ccgaagagat cgcgccgggc
     3001 tatcgcagtt ccaccgcatc ctatgtg
//
LOCUS       DASZZC010000274         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1117017,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000274 DASZZC010000000
VERSION     DASZZC010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117017"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VHE37_05805"
     CDS             complement(<1..866)
                     /locus_tag="VHE37_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT35075.1"
                     /translation="MKFLPLVWSMLWHRRARTLLTASSIAAAFLLYGMLQAVGAVLTL
                     GPKASSANALITSHRYGMGKSLPYAYRAQIEAVPGVTFAGPTVFVPFIYQEASTGTQP
                     SLGLDPEFFAHDARFVVDAATLQAFKNKRSALIVGRELAQKYGWKTGDHVPLKSPYIK
                     RRDGAPYWEFDVVGEFHYNADLLGEGISAMRTFARYDYLDEGRRDPGGVNMYMVRVAD
                     PAQATDVARQIDALFQNSANPTKTQTEAEQQRSMLAQIGDVGIIIRAILSAVFFTLVV
                     VAGNTMMRSFRER"
     gene            1062..1138
                     /locus_tag="VHE37_05810"
     tRNA            1062..1138
                     /locus_tag="VHE37_05810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1096..1098,aa:Arg,seq:ccg)
     gene            1343..2125
                     /locus_tag="VHE37_05815"
     CDS             1343..2125
                     /locus_tag="VHE37_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35076.1"
                     /translation="MHALLAGLLVAACAASARAGITDADRLNAQRDAYLERPRGNAAD
                     PPFLLQSSEESDLVSGEVYARAEYALGELAESLSDPRQWCDLLLLHPNTARCAVDGEV
                     DEPKLELEVTRRIDSPETHHLAFSLRAAASTPAYFGADLAAEHGPMATSDYRIRVEAV
                     PVDDKSLFLHLRYSYRVAWSTRLALKMWLATLGRDCVGFTPAGVSRRGEVEYIRGMRG
                     SAERNVMRYFLAIANAAWVSGWKTWRAIRASWRTRKSRRISK"
     gene            2276..2707
                     /locus_tag="VHE37_05820"
     CDS             2276..2707
                     /locus_tag="VHE37_05820"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HVT35077.1"
                     /translation="MKKAAARKPASPGGPSASALIDARIADLGDWRGETLSRMRQLIH
                     EAEPQVEEHWKWMGTPVWECDGIICTGESYKAVVKMTFAKGASLKDPKKLFNSSLDGN
                     VRRAIDIREGEQVNAAAFKALVREAIALNAAGRKTRSHRAK"
     gene            2736..3560
                     /locus_tag="VHE37_05825"
     CDS             2736..3560
                     /locus_tag="VHE37_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35078.1"
                     /translation="MTLRIVRVWVAFALLAAAQLASGHDYQTFDSDGCQIAYTDYGSG
                     EPVIFLHGFSGNFDQYLGPVGDALSQQFRAIGLDQRGHGRSCKPHDADAYGRHFAGDV
                     LNLMDRLHIAKAHVVGHSMGGIVAMYLAANYPARVQSAVTVGNGLFSRRELTLIGWLM
                     RGSFAWRHVKEFFGAADDNRPPGNDDVALILAVRSLRDLAVTNAQAAALKVPVLAVRG
                     GPKDDPNDTVERLSAINPNVEMLRLEPEDHISTLANAKFRQELKSFLERRSVRAGS"
     gene            complement(3698..>4406)
                     /locus_tag="VHE37_05830"
     CDS             complement(3698..>4406)
                     /locus_tag="VHE37_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35079.1"
                     /translation="QEHYSDNGNYPAGFFASIAAGTCNTYMGCGNYTNWVPAGAQVAS
                     YNSSPSYSWALYKDDSSYYQNYYATQIQANTPLGDVSPWDLGYQNLMLASSVSSSQLP
                     NTANGSNLDPNAAEYMLLSQGSDSNFGRGEVGLHAGALSSKSYLIELLTGQDLDTSRT
                     HTGATNHVGMLTDDELRVLKGVIDIGFPYMSRCDGQSYEAQQSPATVPSGLVRSGPYQ
                     GHPWGDPQVRQLPAGTF"
BASE COUNT          762 a         1406 c         1496 g          742 t
ORIGIN      
        1 cgctcgcgga acgagcgcat catggtgttg cccgccacca ccaccagcgt gaagaacacc
       61 gccgagagaa tggcgcggat gatgatgccc acgtcgccga tctgcgccag catcgagcgc
      121 tgctgctcgg cctcggtctg cgtcttggtc gggttggccg agttctggaa cagcgcgtcg
      181 atctgccggg cgacgtcggt cgcctgcgcc ggatcagcca cgcgcaccat gtacatgttc
      241 acgccaccgg ggtcgcgccg gccctcgtcc aggtagtcgt agcgcgcgaa agtgcgcatg
      301 gcgctgatgc cttcgccgag caggtcggcg ttgtagtgga actcgcccac cacgtcgaac
      361 tcccagtacg gcgcgccgtc gcgccgcttg atgtacggcg atttcagcgg tacgtgatcg
      421 ccggttttcc aaccatattt ctgcgccagt tcgcggccga cgatcagcgc ggagcgcttg
      481 ttcttgaagg cctgcagcgt ggccgcgtcg accacgaagc gcgcatcgtg cgcgaagaac
      541 tccgggtcca accccagcga aggctgggtg ccggtactgg cttcctggta gatgaacggc
      601 acgaacacgg tcggcccggc gaaggtgacg ccgggcaccg cctcgatctg cgcgcggtag
      661 gcatagggca gcgacttgcc catgccgtag cggtgcgagg tgatcagcgc gttggcgctg
      721 gacgccttcg gccccagtgt cagcaccgca ccgaccgcct gcagcatgcc gtagagcagg
      781 aacgctgcgg cgatggacga tgccgtgagc agcgtacgtg cgcgccggtg ccacagcatc
      841 gaccagacca gcggcaggaa tttcatgcga tgagcataac cgggaaggga actgcttcga
      901 gtaaaatgcg gcggcattgc aagctcgatg cgcccgtagc tcagctgggc gcgccgcacg
      961 tggtacgcga agcattgctt cgcgtgcggc gcgcgacccg ccagggatgg cggggccgga
     1021 accaagcgcc agggacggct tgagcgataa tgtctgcaga tgcgcccgta gctcagctgg
     1081 atagagtgcc ggtttccgaa accgtaggtc gcaggttcga atcctgccgg gcgcgccaac
     1141 cagttcttcc gtcgttcccg gattcgcacc tgctgcgccc gtggcccagg ccatccgcag
     1201 tcagggaact gacttgcgac cttgacgcca gctacacgca cgcgcaactt gtgctgtcca
     1261 tgctgatggc aacgtgcgga aaacttcatc gccagcgatc cgtcgacggg gacaacggca
     1321 ggggctacga cgtgcctggc atgtgcatgc gctgctggcg ggattgctcg tcgcggcatg
     1381 tgccgcaagt gcgcgcgccg gaatcaccga cgccgaccgt ctcaacgcgc agcgcgacgc
     1441 atacctcgag cgcccgcgcg gcaacgcggc cgacccgccg ttcctgctgc agtccagcga
     1501 ggaaagcgac ctggtcagcg gcgaggtcta cgcgcgcgcg gaatacgcgc tcggcgaact
     1561 ggctgaatcg ctcagcgatc cgcggcagtg gtgtgacctg ctgttgctgc atcccaacac
     1621 cgcccgctgt gcggtggatg gcgaggtgga tgagccgaag ctggaactgg aggtgacacg
     1681 ccggatcgat tcccccgaga cgcatcatct cgccttcagc ctgcgcgccg ctgcatcgac
     1741 tccggcctac ttcggcgccg acctcgcagc agagcacggg ccgatggcca ccagcgatta
     1801 ccgcatccgc gtcgaagcgg tcccggtcga cgacaagtcg ctgttcctgc acctgcgcta
     1861 cagctaccgc gtcgcctggt cgacgcggct ggcgctgaag atgtggctcg ccacgctggg
     1921 ccgcgactgc gtgggcttca cgcccgcggg agtctcgcgc cgcggcgagg tggaatacat
     1981 ccgcggcatg cgcggctccg ccgagcgcaa cgtcatgcgc tatttcctgg cgatcgcgaa
     2041 cgcagcctgg gtttctggct ggaagacgtg gcgcgctatc cgcgccagct ggaggacgag
     2101 gaaatcccgc cgtatctcca agtgaagacg cgccagcatc catcccgggc ggcgccggcg
     2161 gtggccgcga ggtaagcgcg cgccggcctc atccggcaac tccttcagcg gctgcgcgat
     2221 atatttcccg cccttgggaa tgcccgcgtt cgggcggcag cttcaggagg agaacatgaa
     2281 aaaggcggcg gcacggaaac cagcttcgcc cggcggtccg tcggcctcag ccttgatcga
     2341 cgcgcggatc gccgacctcg gcgactggcg cggcgaaacg ctgagccgca tgcgccagtt
     2401 gatccacgaa gccgagccgc aggtggagga gcactggaaa tggatgggca cgccggtgtg
     2461 ggaatgcgac ggcatcatct gtaccggcga gtcgtacaag gccgtggtga agatgacttt
     2521 cgccaagggt gcgtcgctga aggacccgaa gaagctgttc aactcgagtc tcgacggcaa
     2581 tgtgcggcgg gccatcgata tccgcgaagg cgagcaggtg aatgcggcag cgttcaaggc
     2641 gctggtccgc gaggcgatcg cgctcaatgc cgcgggtcgc aagaccaggt cgcaccgggc
     2701 gaagtagaca ggcgcgcctg agaaactaca cgtacatgac acttagaatc gtgcgcgtgt
     2761 gggtcgcctt tgccctgctg gccgcggcgc aactggcgtc cggccacgat taccagacct
     2821 tcgattccga cggctgccag atcgcctaca ccgactatgg ttcaggcgag ccggtgattt
     2881 tcctgcacgg cttcagcggc aacttcgacc agtacctcgg cccggtcggc gatgcactaa
     2941 gccagcagtt ccgcgcgatc ggcctcgacc agcgcggcca tggacgcagc tgcaaacctc
     3001 acgacgccga cgcctatggc cggcatttcg ccggcgatgt cctgaacctg atggaccggc
     3061 tgcacatcgc gaaagcgcac gtggtgggac attccatggg cggcatcgtt gccatgtacc
     3121 ttgcggcgaa ctatcccgcg cgcgtgcagt cggcggtgac cgtgggcaat ggcctgttct
     3181 cccggcgcga gctcacgctg atcggctggc tgatgcgcgg cagttttgcc tggaggcacg
     3241 tgaaggaatt cttcggcgca gccgacgaca accgccctcc cggcaatgac gatgtggcgc
     3301 tgatcctggc ggtgcgcagc ctgcgcgatc ttgccgtcac caacgcccag gcggcggcgc
     3361 tgaaggtgcc ggtgctcgcg gtccggggcg ggccgaagga cgatccgaac gacacggtcg
     3421 agcggctttc ggcgatcaac ccgaacgtcg agatgctcag gctggagccg gaagatcaca
     3481 tcagcacgct ggccaacgcg aaattccggc aggaactgaa gtcgttcctg gagcgcagga
     3541 gcgtccgcgc gggcagctag cgcgccgcgg ccagtgccct gcgcagcggc gccgtcgatg
     3601 agatagcgca cggtgaggcc gccgatgatg atggtttggt gagcgccgct cgccgtggcc
     3661 attctccatg gcgcaaccga cctggtcgcg gcggatatca gaacgtgccg gcaggaagct
     3721 gtcgtacctg cgggtcaccc cacggatgtc cctggtacgg ccccgagcgc accaggcccg
     3781 acggcacggt ggccggtgat tgctgcgctt cgtagctctg gccgtcgcag cggctcatgt
     3841 aggggaagcc gatgtcgatg acgcccttga gcacgcgcag ttcgtcgtcg gtcagcatgc
     3901 cgacatgatt ggtggcaccg gtatgggtgc gcgaggtgtc gagatcctgc ccggtcagca
     3961 gttcgatgag gtagcttttc gacgacagcg cgccggcgtg caggcccact tcgccgcggc
     4021 cgaaattgct gtccgagccc tgcgacagca gcatgtattc ggctgcgttc ggatcgaggt
     4081 tggagccgtt ggcggtgttc ggcagctggc tcgacgacac gctcgaagcg agcatcaggt
     4141 tctggtagcc gagatcccag ggtgaaacat cgccgagcgg cgtgttggcc tggatctgcg
     4201 tggcgtagta gttctggtag tagctgctgt cgtccttgta gagcgcccag ctgtacgacg
     4261 gcgacgagtt gtagctcgcc acctgcgcgc ccgccggaac ccaattggtg tagttgccgc
     4321 agcccatgta ggtgttgcaa gtgccggcgg cgatgctggc gaagaatccc gcgggataat
     4381 tgccattgtc cgagtaatgc tcctgc
//
LOCUS       DASZZC010000275         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_24700,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000275 DASZZC010000000
VERSION     DASZZC010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24700"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1047
                     /locus_tag="VHE37_05835"
     CDS             151..1047
                     /locus_tag="VHE37_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT35080.1"
                     /translation="MQLRIRDLSKRYANGVQALNQVSLDIAPGMFGLLGPNGAGKSTL
                     MRTLATLQQADGGSATLGDIDLLREPGRAREVLGYLPQDFGVYPKVSALDLLDHLARL
                     KGYAESGPRRATVEALLQRVNLYEVRKQKLGGFSGGMRQRFGIAQALLGAPRLIIVDE
                     PTAGLDPEERVRFHNLLADIAQDVVVILSTHIVDDVSDLCSRLAIINKGRVLLSGEPA
                     ALVESVRGRIWRKVADKGEVEALKAGAPVISTRLSAGRTLVRACADAAPGAGFEAAEA
                     DLEDVYFSAIAGHLAPAPALAA"
     gene            1055..>1500
                     /locus_tag="VHE37_05840"
     CDS             1055..>1500
                     /locus_tag="VHE37_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT35081.1"
                     /translation="MRTLLAIAGFELRSRLKMPSTYAYFALYVAIAAFWMAAAGGAVD
                     SAQISFGSEKVLINGPYALAQLLTLLGFMGISVIAAMMGRAVQQDFEHGTYHFFFSAP
                     IAKRDYVLGRFLGAYLTLLLVFLGVAAGALIGLHWPGVDATRVGPTT"
BASE COUNT          225 a          530 c          502 g          243 t
ORIGIN      
        1 atttttatat ataaataata ttttataaca cacgaaacag ccgtgcggtg acggaaaaaa
       61 tgcgtgcgca gcttaacgcg caagcacccg gcgggcgcgg cgggctgaat tacactcggc
      121 gccatggttc gcgttaccgg gaaggaactc atgcaactgc gcatccgcga tctatccaag
      181 cgctacgcca acggcgtgca ggcgctcaac caggtgtcgc tcgacatcgc gcccggaatg
      241 ttcgggctgc tcggccccaa cggcgccggc aagtccacgc tgatgcgcac gctggccacg
      301 ctgcagcagg ccgacggcgg cagcgccacg ctgggcgaca tcgacctgct gcgcgaaccg
      361 ggccgcgccc gcgaggtgct cggctacctg ccgcaggatt tcggcgtgta tccgaaagtc
      421 tccgcgctcg acctgctcga ccacctggcg cggctcaagg gctacgccga gtccggcccg
      481 cggcgcgcca ccgtcgaagc gctgctgcag cgggtgaacc tgtacgaggt gcgcaagcag
      541 aagctgggcg gattttccgg cggcatgcgc cagcgcttcg gcatcgcgca ggcgctgctg
      601 ggcgcgccgc ggctgatcat cgtcgacgag cccaccgccg gcctcgaccc cgaggagcgc
      661 gtgcgcttcc acaacctgct ggccgacatc gcgcaggacg tggtggtgat cctgtcgacg
      721 cacatcgtcg acgacgtgtc cgacctgtgc tcgcgcctgg ccatcatcaa caagggccgc
      781 gtgctcttgt cgggcgagcc ggccgcgctg gtggaatcgg tgcgcggccg catctggcgc
      841 aaggtggccg acaagggcga ggttgaagcg ctcaaggccg gcgcgccggt catctccacg
      901 cggctgtcgg cggggcgcac gctggtgcgc gcctgcgccg atgccgcgcc cggcgccggc
      961 ttcgaggccg ccgaggccga tctggaagac gtctatttca gcgccatcgc cgggcatctc
     1021 gcgccggcgc ccgcgctggc cgcgtaggcg cgccatgcgc acactgctgg ccattgcggg
     1081 gttcgagctg cgctcgcggc tgaagatgcc gtccacctac gcctacttcg cgctgtatgt
     1141 cgccatcgct gctttctgga tggcggcggc cggcggcgcc gtcgacagcg cgcagatcag
     1201 cttcggcagc gagaaggtgc tgatcaacgg cccctacgcg ctggcgcagc tgctcaccct
     1261 gctcggcttc atgggcatct cggtcatcgc cgccatgatg ggccgggcgg tgcagcagga
     1321 tttcgagcac ggcacctacc acttcttctt cagcgcgccc atcgccaagc gcgactacgt
     1381 gctcggccgc ttcctcggcg cctacctcac gctgctgctg gtgttcctgg gcgtcgccgc
     1441 cggcgcgctg atcggcctgc actggcccgg cgtcgacgcc acccgcgtgg gcccgaccac
//
LOCUS       DASZZC010000276         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_472740,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000276 DASZZC010000000
VERSION     DASZZC010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472740"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VHE37_05845"
     CDS             complement(<1..514)
                     /locus_tag="VHE37_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35082.1"
                     /translation="MVAACGSSSSSSTPVASSDQDVPETGLQGQVPLADQNSGASAQG
                     YHKGLRLVGQNSIMDRGANFSLAWLDDCAYVSTTSPGQIFGPSSSPYVDPQYNVLNGM
                     AVIDAADPHNPTLIDILQSPAMLAPHESLQANQARHIIVGTRGGGTAFDVYDASDCRH
                     PVLKSSITIGQ"
     gene            794..>1922
                     /locus_tag="VHE37_05850"
     CDS             794..>1922
                     /locus_tag="VHE37_05850"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HVT35083.1"
                     /translation="MGRRRVTRKNKGGLSRRSFLKGAGAASALLATQPFLGAGAQDDD
                     VSSSAAAGSSPFHHGVASGDPRSERVMLWTRVTLAAPPDSIPVTYVVATDTALANIVT
                     SGTASADAAHDYTVKVDVAGLSPGTTYYYRFSANGMDSQIGRTRTLPVGSVDRLRIAV
                     LSCASLAHGYFNAYRCVAHRADLDLVVHLGDYIYEYGDGDYGSARNYQPPHEIITLHD
                     YRTRHNQYKRDPDVKALHRQHPVVAIWDDHEFADNAWLGGAANHQPDTEGAWTDRVNA
                     AVQAYYEWMPIRPPASGDIRNIYRSFRIGDLAELFMLEERITARDQQLDSNLPGGTSL
                     FTESGAYLDTSRHMISNTQQNWLLNHMHQSTAQWKLIGQGVV"
BASE COUNT          328 a          657 c          641 g          296 t
ORIGIN      
        1 cctggccgat ggtgatcgac gacttcagca ccgggtggcg gcagtcgctg gcgtcgtaca
       61 cgtcgaacgc ggtgccgccg ccgcgcgtgc ccacgatgat gtgccgcgcc tggttggcct
      121 gcagcgattc gtgcggcgcc agcatggccg ggctctgcag gatatcgatc agcgtggggt
      181 tgtgcggatc ggcggcgtcg atcaccgcca tgccgttgag cacgttgtac tgcgggtcga
      241 cgtacggcga tgagctcggc ccgaagatct ggccgggcga cgtggtggac acgtacgcgc
      301 agtcatccag ccaggccagt gaaaaattgg cgccgcggtc catgatgctg ttctgcccca
      361 ccaggcgcag gcccttgtgg taaccctgcg ccgaggcgcc gctgttctgg tccgccagtg
      421 gcacctggcc ctgcaggccg gtttcgggca cgtcctggtc gctggaggcg acgggtgtgc
      481 tggacgacga tgaactgccg caggcggcga ccatgcaggc gacaagcatt ccggaaaggg
      541 cgcacgaaaa cgctctcggc aaaattcggt tcatcgccag ctcccttgct cgcgggcgcc
      601 gcatgcgccg gtcgagcccg agtttaggag actcggagcc tgcggtgcag cggctattcg
      661 gcggatgcgt gtgagcattc gccgctctgc tattgcgcgg cgtcgtggct ggcagccctt
      721 gctgcatgcg tccatcgaac cgccaacccg cgcgcgactc taactcgtca ctaaacgacg
      781 acgaggagtc gcgatgggcc gcagacgggt cacccgcaag aacaagggcg gcctgtcgcg
      841 ccgcagcttc cttaagggcg cgggcgcggc ttccgcgctg ctggcgacgc aaccgttcct
      901 gggcgccggc gcgcaggacg atgacgtcag cagctccgct gccgcaggca gcagtccatt
      961 ccatcatggc gttgccagcg gcgatccgcg cagcgagcgg gtgatgctgt ggacgcgcgt
     1021 gacgctcgcc gcgccgcccg acagcatccc ggtgacctac gtcgtcgcca ccgacaccgc
     1081 tctggccaac atcgtcacca gcggcacggc gagtgccgac gcggcgcacg actacacggt
     1141 gaaggtcgac gtggccggcc tctcgcccgg caccacctac tactaccgct tcagcgccaa
     1201 cggcatggat tcgcagatcg gccgcacgcg cacgctgccg gtgggcagcg tcgaccgcct
     1261 gcgcatcgcg gtgctgtcgt gcgcctcgct ggcgcatggc tacttcaacg cctaccgctg
     1321 cgtggcgcac cgcgccgacc tcgacctggt ggtgcacctg ggcgactaca tctacgagta
     1381 cggtgacggt gattacggca gcgcgcgaaa ttaccagccg ccgcacgaga tcatcacgct
     1441 gcacgactac cgcacgcgtc acaaccagta caagcgcgat cccgacgtga aggcgctgca
     1501 ccggcagcac ccggtggtcg ccatctggga cgatcacgaa ttcgccgaca acgcgtggct
     1561 gggcggcgca gccaatcacc agccggacac cgaaggtgcg tggacggacc gcgtgaacgc
     1621 ggcggtgcag gcctactacg agtggatgcc gatccggccg ccggccagcg gcgacatcag
     1681 gaacatctac cgctcgttcc gcatcggcga cctggcggaa ctgttcatgc tggaagagcg
     1741 catcaccgcg cgcgaccagc agctggacag caacctgccg ggcggcacct cgctgttcac
     1801 cgagagcggc gcctacctcg acacctcgcg ccacatgatt tccaacacgc agcagaactg
     1861 gctgctcaac cacatgcacc agtcgaccgc gcagtggaag ctgatcgggc agggcgtggt
     1921 ga
//
LOCUS       DASZZC010000277         3496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_986604,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000277 DASZZC010000000
VERSION     DASZZC010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986604"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..497
                     /locus_tag="VHE37_05855"
     CDS             270..497
                     /locus_tag="VHE37_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35084.1"
                     /translation="MLSKTAYDVLPYAYFGAAVLDVALLHSPLKYVPAVLLVLAGVMV
                     ISWRKAAREKQRRRTAQKAASRHAHLTPWPG"
     gene            552..1442
                     /locus_tag="VHE37_05860"
     CDS             552..1442
                     /locus_tag="VHE37_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HVT35085.1"
                     /translation="MSAFLLIAASFALGLLLRRAMPAGSSAALNAWIIDIALPALVLL
                     KIPQLHFSPALVFPALAPWLVFAGAWLLLARMGALRNWPRGTIGALVLTAGLGNTAFV
                     GIALVEALRGSDAVGPAVVADQLGTFLALSTGGIGAAAYYAGASVAPRDMAARVLRFP
                     PFIALCVAALAGVSGGWPPLLVPVLQRLADTLVPLALFSVGLQFQLRGIGDDLGRMSL
                     AMAWKLALAPALVAACGYLSGVGGHAYAASVLQCAMAPMVSAGILAEYHGLDPKLANR
                     MVSLGTVLSFATVPLWSLML"
     gene            complement(1447..2634)
                     /locus_tag="VHE37_05865"
     CDS             complement(1447..2634)
                     /locus_tag="VHE37_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT35086.1"
                     /translation="MLLTHEHKELYKTVKKFVEDEINPHVEEWEKAGIWPAHEVLKKM
                     GQLGLLGISRPEAYGGMGLDYSYEVVFALALGHCACGGVPMGIGVQTDMATPALARFG
                     SDALKKEYLAPAIAGDMVVSIAVSEAGAGSDVASIKTTARKDGGDYVINGSKMWITNG
                     TQSDWACMLVNTSEGKVHQNKSLIIVPMDTKGIDRKTKLDKLGMRSSDTAMIFLDNVR
                     VPQRNLIGQEGMGFMYQMLQFQEERLFGAASGIEGLNNIIEQTIAYTGQRQAFGQSLL
                     DNQAVHFRMAELKTEVEALRAMVFQATEEYVAGRDATMLASMCKLKMGRLGREVTDAC
                     LQYWGGQGYMWETPVARAYRDTRLISIGGGADEIMLGIIAKLMGILPSKKKHEQNKAN
                     RAA"
     gene            complement(2702..>3496)
                     /locus_tag="VHE37_05870"
     CDS             complement(2702..>3496)
                     /locus_tag="VHE37_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HVT35087.1"
                     /translation="IKRRLQQMVLALFFSCSFYARAGDPPPRELHYSVVRSYVHDAAR
                     FTQGFAIRDGVLYESTGRYGLSALYAQDLRSGQLLRTRKNTPDVFAEGLAVLGPRVFQ
                     LTWRERRGYIYDRSFKPLGEFDYGTEGWGLTEDGTHLIMSDGSALLTWLDANTLQREH
                     DVVVRDQGAPVERLNELEYVNGMVWANVWLTDRIAAIDAASGAVRGWLDLSALKKGFA
                     RPAGWDESDNVLNGIAYDPASKHFFVTGKCWPQMFELQVDAPPPAQ"
BASE COUNT          491 a         1166 c         1181 g          658 t
ORIGIN      
        1 ccggcagcga aagcgtggtg gtgctgccgg cgcgccagta cccgcagggt tacaccgcca
       61 cggtccaggg cggcagcgtg gtctcggcgc cgggcgcgcc gctgctgcgc atcgcagcgc
      121 aggccgacga agtcaggctg accgtggtcc ccgcgcgata gacggcaacg accggggcat
      181 ggccggaggg ggcggtattt cgcgcccaca cagcggtgca taactcctgg gtagcatgac
      241 ttccatcaca actgagggaa gtgaccgccg tgctcagcaa gaccgcctac gacgtattgc
      301 cctacgccta cttcggtgcc gccgtgctcg acgtggcctt gctgcactcg ccgctcaagt
      361 atgtacccgc ggtgctgctg gtcctggccg gcgtgatggt gatcagctgg cgcaaggcgg
      421 cgcgcgagaa gcagcgcagg cgcaccgcgc agaaagcggc gtcgcgccac gcgcatctca
      481 cgccctggcc gggctgaaga ttaactgaga cctgactcgg cgcactgagg cgcgcgctac
      541 actcgcgcgc catgagcgcg ttcctgctga ttgccgcgag cttcgcgctc ggactcctgc
      601 tgcgccgtgc catgccggcg ggcagcagcg cggcgctcaa tgcctggatc atcgacatcg
      661 cgctgccggc gctggtgctg ctgaagatcc cgcagctgca tttttcaccc gcactggttt
      721 tccccgcgct cgcgccgtgg ctggtgttcg ccggcgcctg gctgctgctg gcgcgcatgg
      781 gcgcgttgcg caactggccg cgcggcacca tcggcgcact ggtgctcacg gccgggctgg
      841 gcaacacggc gttcgtcggc atcgcgctgg tggaagcgct gcgcggcagc gacgccgtgg
      901 ggccggcagt ggtcgctgac cagctgggca cctttctcgc gctgtccacc ggcggcatcg
      961 gcgccgcggc gtactacgcc ggcgccagcg tggccccgcg cgacatggcg gcgcgcgtgt
     1021 tgcgttttcc gccgttcatc gcgctgtgcg tggcggcgct cgcgggcgta tctggcggat
     1081 ggccgccgct gctggtgccg gtgctgcagc gcctggccga cacgctggtg ccgctcgcgc
     1141 tgttttcggt gggcctgcag ttccagctgc gcggcatcgg tgatgacctt ggccgcatgt
     1201 cactggccat ggcgtggaag ctcgcgctgg cgcctgcgct ggtcgcggcc tgcggatatc
     1261 tttcgggtgt tggcgggcac gcgtacgcgg caagcgtcct gcaatgcgcg atggcgccga
     1321 tggtctcggc cggcatcctg gccgaatacc acgggctcga tccgaagctg gccaaccgca
     1381 tggtcagcct gggcacggtg ctgtcattcg ccaccgtgcc cttgtggtcg ctgatgctct
     1441 gacctctcag gccgcccggt tggccttgtt ctgctcgtgc ttcttcttgc tcggcaggat
     1501 gcccatcagc ttggcgatga tgccgagcat gatctcgtcg gcgccgccgc cgatggagat
     1561 caggcgcgtg tcgcggtacg cgcgcgccac cggcgtctcc cacatgtagc cctggccgcc
     1621 ccagtactgc aggcaggcgt cagtgacttc gcggccgagc cggcccatct tcagcttgca
     1681 catggaggcg agcatggtgg cgtcgcgtcc ggccacgtat tcctcggtgg cctggaacac
     1741 catggcgcgc agcgcctcga cctcggtctt cagctcggcc atgcggaaat gcaccgcctg
     1801 gttgtcgagc agcgactggc cgaacgcctg gcgctggccg gtataggcga tggtctgttc
     1861 gatgatgttg ttcaggcctt cgatgcccga ggcggcgccg aacaggcgct cttcctggaa
     1921 ctgcagcatc tggtacatga agcccatgcc ttcctggccg atcaggttgc gctgcggcac
     1981 gcgcacgttg tccaggaaga tcatcgcggt gtcggacgag cgcatgccca gcttgtccag
     2041 cttggtcttg cggtcgatgc ccttggtgtc catcggcacg atgatcaggc tcttgttctg
     2101 gtgcaccttg ccttcggagg tgttgaccag catgcaggcc cagtccgact gcgtgccgtt
     2161 ggtgatccac atcttgctgc cgttgatcac gtagtcgccg ccgtccttgc gcgcggtggt
     2221 cttgatgctg gccacgtccg aaccggcgcc ggcttccgac actgcgattg acaccaccat
     2281 gtcgccggcg atggcgggtg ccaggtattc cttcttcagg gcatcggagc cgaagcgcgc
     2341 cagcgccggc gtggccatgt cggtctgcac gccgatgccc atcggcacgc caccgcaggc
     2401 gcagtgcccc agcgccagcg caaacaccac ttcgtaggag taatcgaggc ccatgccgcc
     2461 gtacgcttcg ggccgggaga tgccgagcag gccgagctgg cccatcttct tcagcacttc
     2521 gtgtgccggc cagatgccgg ccttctccca ctcctcgacg tgcggattga tttcgtcctc
     2581 gacgaatttc ttgaccgtct tgtacagctc tttgtgttcg tgcgtaagca gcattaccag
     2641 tctccgggcg cggtgcgcgg cgagtgtagc gcggcgctgc acgcgctgcg ggtaagcgcg
     2701 cttactgtgc gggtggtggc gcgtcgacct gcagttcgaa catctgcggc cagcacttgc
     2761 cggtgacgaa gaaatgcttc gacgccgggt cgtacgcgat gccattgagc acgttgtcgc
     2821 tctcgtccca gcccgcaggc ctggcgaaac cctttttcag cgcggacagg tcgagccagc
     2881 cgcgcaccgc gccactggcg gcgtcgatcg cggcgatgcg gtcggtgagc cacacgttgg
     2941 cccagaccat gccgttcacg tattcgagtt cgttgagccg ctccaccggc gcgccctggt
     3001 cgcgcaccac cacgtcgtgt tcgcgctgca gcgtgttggc gtcgagccag gtgagcaaag
     3061 cgctgccgtc gctcatgatc aggtgcgtgc cgtcttcggt caggccccag ccctcggtgc
     3121 cgtagtcgaa ctcgcccagc ggcttgaagc tgcggtcata gatatagccg cgccgctcgc
     3181 gccaggtgag ctggaatacg cgcgggccga gcaccgcgag cccttcggcg aaaacatccg
     3241 gcgtgttctt gcgcgtgcgc agcagctggc cgctgcgcaa gtcttgcgcg tagagcgcgg
     3301 agaggccgta gcggccggtg ctttcgtaca acacgccgtc cctgatggcg aagccctggg
     3361 tgaagcgcgc agcgtcatgc acatagctgc gcaccacgct gtagtgcagt tcgcgcggtg
     3421 gcggatcgcc ggcgcgcgcg taaaaactgc agctgaaaaa gagggccaaa accatttgct
     3481 gcagcctgcg ttttat
//
LOCUS       DASZZC010000278         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_598927,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000278 DASZZC010000000
VERSION     DASZZC010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598927"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VHE37_05875"
     CDS             complement(<1..557)
                     /locus_tag="VHE37_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35088.1"
                     /translation="MYFFAASAQAEPAFARLYKQQYGYPPSCNACHKDGGGTPLNPYG
                     QQFKDAGMNAAAFDKIAKNDADGDGALNGDEAAAKSNPGDKTSTPASKGNWLDTASLI
                     PREVQAQFPEVRAYLPQDAILTQAEFDRAKTLGAELSKADENTIYIPLVDQHPAGTAI
                     IFPASYRGKDFFLLLATDRQLKVTAV"
     gene            complement(669..2384)
                     /locus_tag="VHE37_05880"
     CDS             complement(669..2384)
                     /locus_tag="VHE37_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35089.1"
                     /translation="MKRALWAAVIAALLLAAWLLLRPGEGGGPDISARAKQVPPPAAL
                     TLSSQFISADDLPPDGTRSLFDHLVAQNQSLPYPFPKLVEMVRRSDPQNAPPLTLLIP
                     QGRSLLKASADDAHPRVLAAADFQAADTPAGLGLAPRGQLFLGFVEQAHEIEVLSYNE
                     AAGRYEFQLVQNYCDGCVPRIVYARRAICTTCHQSAVPIFPQRPWNETNGQTVTAEHI
                     AAARGNEPYLGVPVQNALGVPERFDELTDVGMFNVVTQRTWIDGCAQSADCRRQQLKV
                     ALEYLWSPAQFDEHGANAQRLRDLQKASWPATGIVVPNNDLPNRDPVAETQGIKGFFR
                     SLFTRHPEPGAGAKDNEDLAAFDRLPKLPASLDPLTPRAPKRTLTADDLDGAYGIAAL
                     FTESDFARLEAASGHDLAKLLGAVDGLDAGVLKAQVYARVRMMNALLAALRAPAPLDY
                     CCWSTAEMSPPVASGEPPVKIADGSVLLEFQRYCFACHRGNPSAKLNFMAGRSEVEVL
                     EHIKAKSEIRDALDWQRYKGTDKENKLMPPADSAQRAALAADAQKDPKLLGRMRDTVP
                     GLFDF"
     gene            complement(2392..>4465)
                     /locus_tag="VHE37_05885"
     CDS             complement(2392..>4465)
                     /locus_tag="VHE37_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35090.1"
                     /translation="SFAPIPQAAPPPCDQPQPTGYDAESCRYVNSLFKDVAAKDPAKA
                     RAWRERARRGRDVWFKGTFGNQDEIYLHFSRTVPDKIDDIWYPWLDTRERKTRFSKYG
                     VINDPDCTEGDASTNWWDKCPDPHSSGVLGYRRYYADPVKDASGKVVFNPQTSPYEPD
                     ELKHNKRFVIGHPCVQCHVGFDPTNPPADPNEPKWENISATIGNQHINQPTMFFQGAP
                     PDSLARMALEAGRPGTIDTSLVANDFQNNPGTQNNIMDFQNRRVFTNKMKDPVTGEIR
                     EGRTQHVLKGGEDSVGDHLALIRVYVNIGMCTEECWTPNFPTPGSYFGEQAHQRPFRI
                     AQCAKDCEPWNYADAKMDDLAAFLVTGGPTYLMNAKDSDGTAGSSFIDLSKVPQGHKV
                     FARECAGCHSSLVPPENVRGDKAQLAKFFDGHVFGKEDYWQYEFTDAERSAPEFQAKY
                     LVKDAGGKLRPKQFADKEIFGQDWLGNDEPTPFNIIGTNMCRALHDNHSTGHIFEEFS
                     SEDYKARPSPGSVPNVINRMIPLVGGMKVGERKIEGGPGYLRNISLLSVWATAPFLHN
                     NAIGELTYLPDGKTIDYTVRGRIKQFEMAYAELMTSDDPNQPAHRPQKITVTDRDFKL
                     APREDGQGPIKLTVPKGTPVANFASSDPHHPLFMKCDDPVENKGHQFGVSLSPDDKLA
                     LREFLKLM"
BASE COUNT          674 a         1434 c         1509 g          848 t
ORIGIN      
        1 accgccgtca ccttgagctg gcggtcggtg gccagcagca ggaagaaatc cttgccgcgg
       61 tacgaggccg gaaaaatgat cgcggtgccg gcgggatgct ggtcgaccag cgggatgtag
      121 atggtgttct cgtcggcctt ggacagttcc gcgccgagag ttttggcgcg gtcgaactcg
      181 gcctgcgtca ggatcgcatc ctgcggcagg taggcgcgca cctcgggaaa ctgcgcctgc
      241 acctcgcgcg ggatcaggct ggcggtgtcg agccagttgc ccttgctggc tggcgttgaa
      301 gtcttgtcgc ccgggttgga tttcgccgcg gcctcgtcgc cattgagcgc gccgtcgccg
      361 tcggcatcgt tcttcgcaat cttgtcgaac gccgccgcgt tcatgccggc gtccttgaac
      421 tgctggccgt agggattcag cggcgtgccg ccgccatcct tgtggcaggc gttgcatgaa
      481 ggcggatagc cgtactgctg cttgtacagg cgggcgaagg cgggttcggc ctgcgcggat
      541 gccgcaaaaa aatacaccgt gattcccgcg aaggcgggaa tccagtttct ggctgaagct
      601 ggaacggatc gaaaactgga tccccgcctt cgcgggaatg acgaagccaa gattttcatc
      661 gtcgccctct agaaatcgaa caggcccggc accgtgtcgc gcatgcggcc gagcagcttc
      721 ggatctttct gcgcatcggc cgccagcgcc gcgcgctgcg ccgaatcggc cggcggcatc
      781 agcttgttct ccttgtcggt gcctttatag cgctgccagt cgagcgcgtc acggatttcg
      841 ctcttggcct tgatgtgctc cagcacttcc acttcgctcc tgccggccat gaagttgagc
      901 tttgcgctcg gattgccgcg atggcaggcg aagcagtagc gctggaattc gagcagcacc
      961 gagccatcgg caatcttcac cggcggttca ccgctggcca ccggcgggct catctcggcg
     1021 gtggaccagc agcaataatc cagcggcgcc ggcgcacgca gagccgcgag cagcgcgttc
     1081 atcatgcgca cacgcgcgta gacctgcgcc ttcagcactc ccgcgtcgag accgtcgacg
     1141 gcaccgagca acttcgccag gtcatggccg ctcgccgctt ccagccgcgc gaaatcgctc
     1201 tcggtgaaca gcgcggcgat gccataggca ccgtcgagat cgtccgcggt cagcgtgcgc
     1261 ttgggcgcgc gcggcgtcag cgggtcgagc gacgccggca gtttcggcag gcggtcgaag
     1321 gcggcgaggt cctcgttgtc cttcgcaccc gcgcccggct cgggatggcg cgtgaacagc
     1381 gagcggaaaa atcccttgat accctgcgtc tcggcaaccg ggtcgcggtt gggcaggtcg
     1441 ttgttcggca ccacgatgcc ggttgcgggc caactcgcct tctgcaagtc gcgcaggcgc
     1501 tgcgcattcg cgccgtgctc gtcgaactgc gccgggctcc acaaatattc gagcgccacc
     1561 ttcaactgct ggcgacggca atcggcgctc tgcgcgcaac catcgatcca ggtgcgctgc
     1621 gtgaccacgt tgaacatgcc gacgtcggtc agctcgtcga agcgttccgg cacgccgagc
     1681 gcgttctgca ccggcacgcc gaggtagggt tcgttgccgc gcgccgccgc gatgtgctcg
     1741 gccgtcaccg tctgcccgtt ggtctcgttc cacggccgct gcgggaagat cggcactgcc
     1801 gattgatgac atgtcgtgca gatggcgcgg cgcgcgtaga cgatgcgcgg cacgcagccg
     1861 tcgcagtagt tctgcaccag ctggaattcg tagcggccgg cggcctcgtt gtagctcagc
     1921 acttcgatct cgtgcgcctg ctcgacgaag ccgaggaaca gctggccgcg cggcgcgagt
     1981 ccgaggccgg ccggcgtgtc ggcggcctgg aagtcggcag cggccagcac gcgcggatgg
     2041 gcgtcgtcgg cactggcctt gagcagcgag cggccctgcg ggatgagcaa ggtgagcggc
     2101 ggcgcgtttt gcggatcgct gcggcggacc atctcgacca gttttgggaa cggatacggc
     2161 agcgactggt tctgcgccac caggtgatcg aacagcgagc gcgtgccatc cggcggcaga
     2221 tcgtcagccg agatgaactg cgacgacagc gtcagcgccg caggcggcgg cacctgcttc
     2281 gcgcgcgcgc tgatatccgg cccgccaccc tcgcccggcc gcagcagcag ccacgctgcc
     2341 agcagcagcg cggcgatgac tgcggcccac agcgcgcgct tcatgcgcgg atcacatcaa
     2401 tttcaggaac tcgcgcagcg ccagcttgtc atcgggcgac agcgacacgc cgaactggtg
     2461 gcccttgttc tccaccgggt cgtcgcactt catgaacagc gggtggtgcg gatcggacga
     2521 ggcgaagttg gccaccggcg tgcccttggg cacggtgagc ttgatcggcc cctggccgtc
     2581 ctcgcgcggc gccagcttga agtcgcggtc ggtcacggtg attttctgcg ggcgatgcgc
     2641 gggctggttc ggatcgtccg acgtcatcag ctcggcgtag gccatctcga actgcttgat
     2701 gcgcccgcgc acggtgtagt cgatggtctt gccgtcgggc aggtaggtga gttcgccgat
     2761 ggcgttgttg tgcaggaacg gcgcggtggc ccacaccgac agcagcgaga tgttgcgcag
     2821 gtagccgggc ccgccctcga tttttctctc gcccaccttc atgccgccga ccagcgggat
     2881 catgcgattg atcacgttgg gcactgaacc cggcgacggc cgcgccttgt agtcctcgct
     2941 ggagaactcc tcgaagatat ggccggtgct gtggttgtcg tgcagcgcgc ggcacatgtt
     3001 ggtgccgatg atgttgaacg gcgtcggctc gtcgttgccc agccagtcct ggccgaaaat
     3061 ctccttgtcg gcgaactgct tcggccgcag cttgccgccg gcatccttca ccaggtattt
     3121 cgcctggaac tccggcgcgc tgcgctcggc gtcggtgaac tcgtactgcc agtagtcttc
     3181 cttgccgaac acgtggccgt cgaagaactt ggccagctgc gccttgtcgc cgcgcacgtt
     3241 ttccggcggc accagcgagc tgtggcaacc cgcgcactca cgcgcgaaga ctttatggcc
     3301 ctgcggcacc ttgctcaggt cgatgaacga ggaaccggca gtgccgtcgc tgtccttcgc
     3361 attcatcagg taggtcggcc cgccggtgac caggaacgcc gcgaggtcgt ccatcttggc
     3421 gtcggcgtaa ttccacggtt cgcaatcctt ggcgcactgc gcgatgcgga acggccgctg
     3481 gtgcgcctgc tcgccgaagt agctgccggg cgtggggaag ttcggcgtcc agcattcctc
     3541 ggtgcacatg ccgatgttga cgtacacgcg gatcagcgcg aggtggtcgc ccacgctgtc
     3601 ctcgccgcct ttcaatacgt gctgcgtgcg gccttcgcgg atttcgccgg tcaccgggtc
     3661 cttcatcttg ttggtgaaca cgcgccggtt ctggaaatcc atgatgttgt tctgcgtgcc
     3721 cgggttgttc tggaaatcgt tggccaccag cgaggtgtcg atggtgcccg gacggccggc
     3781 ctccagcgcc atgcgcgcca gcgaatccgg cggcgcgccc tggaagaaca tggtcggctg
     3841 gttgatgtgc tggttgccga tggtggcgga aatgttttcc cacttcggct cgttgggatc
     3901 ggccggcgga ttggtcggat caaaaccgac gtggcactgc acgcacggat ggccaatcac
     3961 gaaccgcttg ttgtgcttca gttcgtccgg ctcgtaaggc gaagtctgcg ggttgaagac
     4021 caccttgccg ctggcatcct tcaccgggtc ggcgtagtac ctgcggtagc cgagcacgcc
     4081 ggacgaatgc ggatcggggc acttgtccca ccagttggtg gacgcgtcgc cctcggtgca
     4141 gtcggggtcg ttgatcacgc cgtacttgct gaagcgggtc ttgcgctcgc gcgtgtccag
     4201 ccacgggtac cagatgtcgt cgatcttgtc cggcaccgtg cgcgagaagt gcaggtagat
     4261 ctcgtcctgg ttgccgaagg tgcctttgaa ccagacgtcg cgcccgcgcc gcgcgcgttc
     4321 gcgccaagcg cgcgccttgg ccggatcttt cgccgccacg tccttgaaca gcgaattgac
     4381 gtagcggcag ctctcggcgt cgtagccggt gggctgcggc tggtcgcacg gcggcggcgc
     4441 ggcctgcggg atcggcgcaa aactc
//
LOCUS       DASZZC010000279         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_401049,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000279 DASZZC010000000
VERSION     DASZZC010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401049"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VHE37_05890"
     CDS             <1..181
                     /locus_tag="VHE37_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HVT35091.1"
                     /translation="VGSGLYGVDLKQIGKQVRVIEVNDNPNIDYGVEDLFLKDQLYRR
                     IMEYFIKRLEARTKT"
     gene            178..648
                     /locus_tag="VHE37_05895"
     CDS             178..648
                     /locus_tag="VHE37_05895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT35092.1"
                     /translation="MKRAAPVRRARAADARAIHALEEHFPSDRMSLRSVRGFLRSPRA
                     QVWVSDGSDLRLHGCLILLTRAHSRKARIYSVVVDPAARGQGLGVRLVQAAQSWARGH
                     GCTQVYLEVREDNHAARALYAKLGYAESARLPGFYDDGADGLRLARPLTRARRR"
     gene            723..1160
                     /locus_tag="VHE37_05900"
     CDS             723..1160
                     /locus_tag="VHE37_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVT35093.1"
                     /translation="MKFPVMAVLLALLPIAAPAAMYRWTDASGAVHYTQTPPPAGTTG
                     TTVAPAPPPGQVSPQLNRLSHYGQQLDQEAQKHAKEQASADDQAQARASQCAQARGRL
                     NLQTAYAGRIFHSDEQGNRQFQSAEENAQVREQLQQQIDENCD"
     gene            1192..1614
                     /locus_tag="VHE37_05905"
     CDS             1192..1614
                     /locus_tag="VHE37_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVT35094.1"
                     /translation="MMLLLPALASAGEMYRWVDKDGTVHYTQTPPPSGTEGADKIKDR
                     APPATAPRSGLQKLSDQYDAEQKKKAEEAAKNAQGADDRAQLCQKARSNLDQMATHPA
                     HRLATNDEQGNPSRMTTEQYDRIKAESENDVRLYCEGE"
BASE COUNT          326 a          584 c          560 g          205 t
ORIGIN      
        1 agtcggcagc gggctgtacg gcgtcgacct caagcagatc ggcaagcagg tccgcgtgat
       61 cgaggtcaac gacaacccga acatcgatta cggcgtcgag gacctgttcc tcaaggacca
      121 gctgtaccgg cgcatcatgg agtacttcat caagcgcctc gaggcgcgca ccaagacgtg
      181 aagcgcgccg caccggtccg ccgcgcgcgt gccgccgatg cgcgtgccat ccacgccctg
      241 gaggaacatt tcccgtccga ccgcatgtcg ctgcgcagcg tgcgcgggtt cctgcgctcg
      301 ccgcgcgcgc aggtgtgggt cagcgacggc agcgacctgc ggctgcacgg ctgcctgatc
      361 cttctcacgc gcgcgcacag ccgcaaggcg cgcatctact cggtggtggt cgatccggcc
      421 gcgcgcggcc agggtctggg cgtgcggctg gtgcaggcgg cgcaaagctg ggcgcgcgga
      481 catggttgta cgcaggtgta cctggaggtg cgcgaggaca accacgccgc gcgcgcgctg
      541 tacgcgaagc tcggctacgc cgaaagcgcg cgcctgcccg gcttctatga cgatggcgcc
      601 gatggcctgc gcctggccag gccgctgacg cgcgcacgcc gccgctgacg cagcagttca
      661 gcgccgcttc atcactgcgc gctaggttga gcgcatggat gctgtacgcc tacactggcc
      721 ccatgaaatt tccggtcatg gccgtgctgc tcgcgctgct gccgatagcg gcgccagcgg
      781 ccatgtaccg ctggaccgac gccagcggcg cggtgcacta cacgcagacg ccgccgcccg
      841 ccggaaccac cggcaccacg gtggcgcccg cgcccccgcc gggccaggtc agcccgcagc
      901 tcaaccgcct gagccactac ggccagcagc tggaccagga agcgcaaaaa catgccaagg
      961 agcaggccag cgccgacgac caggcgcagg cccgcgccag tcaatgcgcg caggcgcgcg
     1021 ggcgcctgaa cctgcagacc gcctatgccg gccgcatctt ccacagcgac gagcagggca
     1081 accgacaatt ccagagtgca gaggagaatg cgcaggtgcg cgagcagctg cagcagcaga
     1141 tcgatgaaaa ctgcgattga aattcagctt ttgaagcgcg ccgccgcggg catgatgctg
     1201 ctgctgccgg cgctggcctc ggcgggcgag atgtaccgct gggtcgacaa ggatggcacc
     1261 gtgcactaca cgcagacgcc gcccccttcc ggcaccgagg gtgccgacaa gatcaaggat
     1321 cgtgcgccgc cggccacggc gccacgcagc gggctgcaga aactgtccga ccagtacgac
     1381 gccgagcaga agaagaaggc cgaggaggca gccaagaacg cacagggcgc cgatgaccgc
     1441 gcccagctat gccagaaggc acgctccaac ctcgaccaga tggccacgca tccggcacat
     1501 cgcctggcga ccaacgacga gcagggcaat cccagccgca tgaccaccga gcagtacgac
     1561 cgcatcaagg cagaatcgga aaacgacgtg cgtctgtact gcgagggcga atgagcggga
     1621 ctgcgcgcgc cggcaaaacc cgcgcgcaat gaaaaacggc cgggagatcc cggcc
//
LOCUS       DASZZC010000280         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1180262,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000280 DASZZC010000000
VERSION     DASZZC010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180262"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VHE37_05910"
     CDS             complement(<1..210)
                     /locus_tag="VHE37_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35095.1"
                     /translation="MDGQGFQFHGTESGRDRREIWQLSAEKNRPRLRVHDTAITLRGG
                     ETHDNHDFSSTDLAPRRGLDPADPAL"
     gene            184..1302
                     /locus_tag="VHE37_05915"
     CDS             184..1302
                     /locus_tag="VHE37_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT35096.1"
                     /translation="MKLETLAIHAGRRVISDTRGIVPAIELGTTFERGADGSFPGGHI
                     YARNSNPNRAALEQCLAALEGGSEAIAFPSGSAATAAVLQALAPGDHLIIPADLYWGT
                     AVIARDLYARWGLQHSMVDLTDLGALQRALTPKTKLVWVETPSNPLLKLTDIRAAVQI
                     AHAANALCVVDSTFATPVLQRPLELGADLVMHSTTKYLGGHSDVLGGALIGRGGEFMA
                     RVRDFQTKAGAAPAPFDCWLLQRSIASLAVRVRAQCDSARRVAEFLERHPRVERVHYP
                     GLPSHAQHALARAQMSSGGAMLSLQVRGGRDEAMGVAARLTIFTRATSLGGVESLVEH
                     RASVEGPDTRTPQNLLRVSIGLEHADDLIADFQQALEG"
     gene            1306..>1604
                     /gene="bamE"
                     /locus_tag="VHE37_05920"
     CDS             1306..>1604
                     /gene="bamE"
                     /locus_tag="VHE37_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HVT35097.1"
                     /translation="MRRLLIACALPLLVLAGCGSRVNADNYAKLKSGMTQDEVHAILG
                     RPAHVTGGGIGGFDVTRETFNGSEQQIRVIFSNGKLLEKSVGSVTDESPADKTDN"
BASE COUNT          256 a          569 c          538 g          241 t
ORIGIN      
        1 tagcgcagga tcggcaggat ccagcccgcg gcgcggcgcc agatcggtac tgctgaagtc
       61 atggttatcg tgtgtctccc cgccgcgcag tgtaatcgca gtgtcatgca cgcgcaggcg
      121 gggtctgttt ttctccgccg aaagctgcca gatttcccgc ctgtccctgc ccgactccgt
      181 cccatgaaac tggaaaccct ggccatccat gccggccgcc gcgtgatcag cgatacgcgc
      241 ggcatcgtgc cggcgatcga gctgggcacc accttcgagc gcggcgccga cggcagcttc
      301 cccggcggcc acatctacgc gcgcaacagc aatcccaacc gcgccgcgct ggaacaatgc
      361 ctggccgcgc tggagggcgg cagcgaggcc atcgcctttc cgtccggctc ggctgcaacg
      421 gcggcggtgc tgcaggcgct ggcgccgggc gatcacctga tcattcccgc ggacctgtac
      481 tggggcaccg cggtcatcgc gcgcgatctc tacgcgcgct ggggcctgca acacagcatg
      541 gtcgatctca ccgacctcgg cgcgctgcag cgcgcgctga cgccgaaaac gaaactggtg
      601 tgggtggaga cgccgtcgaa cccgctgctc aaactcaccg acatccgcgc cgcggtgcag
      661 atcgcgcacg cggcgaatgc gctgtgcgtg gtcgacagca ctttcgccac gccggtgctg
      721 cagcgtccgc tggaactggg cgcggacctg gtgatgcatt ccaccaccaa gtacctgggc
      781 ggccacagcg acgtgctcgg cggcgcgctc atcggccgcg gcggcgagtt catggcgcgc
      841 gtgcgtgact tccagaccaa ggccggcgcg gcgccggcgc cattcgactg ctggctgctc
      901 cagcgcagca tcgccagcct ggcggtgcgc gtgcgtgcgc agtgcgactc cgcgcgccgc
      961 gtggcggaat tcctcgagcg ccacccgcgc gtggagcgcg tgcattaccc gggcttgccc
     1021 tcgcacgcgc agcatgcgct ggcgcgcgcg cagatgagca gcggcggcgc catgctgtcg
     1081 ctgcaggtgc gcggcggccg cgacgaggcg atgggcgtcg ctgcgcggct gaccatcttc
     1141 acccgcgcca ccagcctggg cggggtggaa agcctggtgg agcaccgcgc ctcggtggaa
     1201 ggcccggaca cgcgcacgcc gcagaacctg ctgcgcgttt cgatcgggct ggagcacgcc
     1261 gacgatctca tcgccgattt ccaacaagcc ctggagggct gacccatgcg ccgactgctg
     1321 atcgcctgtg ccctgccgtt gctggtactc gccggctgcg gcagccgcgt gaacgccgac
     1381 aactacgcca aactgaagtc cggcatgacg caggatgaag tgcacgccat cctcggccgg
     1441 ccggcgcatg tcaccggcgg cggcatcggc ggtttcgatg tcacgcgcga aaccttcaac
     1501 ggctccgagc agcagatccg cgtgatcttc agcaacggca agctgctgga aaaatccgtc
     1561 ggctcggtca ccgacgaatc gccggcggac aagaccgaca acaa
//
LOCUS       DASZZC010000281         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_401152,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000281 DASZZC010000000
VERSION     DASZZC010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401152"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VHE37_05925"
     CDS             complement(<1..662)
                     /locus_tag="VHE37_05925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186091.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVT35098.1"
                     /translation="MSSVLIRGARVLDPSSGFDAVASVAVRAGAIATVGKENTKENFD
                     EVIDAKGLWLMPGIVDLAARLREPGATQKATIASETRAALAAGITTVTLPPDTNPVVD
                     TPAVVDRIQSKARAAGALDVRVLGALTKGLAGEALAEMSALKAAGCVGVGNAHFGLRD
                     SLVGRRALEYAQGLDLTVHVFAQNPALANGGCAHEGAVATRLGLPPIPVAAEVSAIRF
                     WIS"
     gene            complement(659..1654)
                     /locus_tag="VHE37_05930"
     CDS             complement(659..1654)
                     /locus_tag="VHE37_05930"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HVT35099.1"
                     /translation="MQPHLQLDASGHLKHLLTLEGLPRDLIVSLLDSAEQFAGAAEGA
                     QKKFEHLRGRTVINLFFEASTRTRTTFELAAKRLSADVLNLQVAASSTSKGETLLDTL
                     KTLEAMHADMFVIRHEASGAAHFFAEHAAPGIAILNAGDGRHAHPTQGLLDLFTIRKA
                     KGPGNECFRKLTVAIVGDVLHSRVARSDIHGLRTLGTKEIRVVAPPTLIPAGIEQMGV
                     KVFRSIDAGLDGADVVILLRLQKERMLGAFLPSLGEFHRDYGLTRARVAQLKKDAVIM
                     HPGPINRGVEIEGDLAYSERSLILQQVSHGIAVRMAVMARLGQARSPSRPKKVRA"
     gene            complement(1770..>1972)
                     /gene="ruvX"
                     /locus_tag="VHE37_05935"
     CDS             complement(1770..>1972)
                     /gene="ruvX"
                     /locus_tag="VHE37_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HVT35100.1"
                     /translation="RRFAAELGQRCKLPVHLCDERFSSRAADDELRSARADGRMARRV
                     RKGDRDGAAARLLLEQWFREGR"
BASE COUNT          298 a          647 c          684 g          343 t
ORIGIN      
        1 gaaatccaga aacggatggc ggagacttcc gccgccaccg ggatcggcgg caggcccagg
       61 cgtgtggcta ccgcgccttc gtgcgcgcag ccgccgttgg ccagcgccgg gttctgcgcg
      121 aacacgtgca ccgtgaggtc gaggccctgc gcgtactcca gtgcccggcg gccgaccagc
      181 gaatcgcgca ggccgaagtg ggcgttgccc acaccgacac agccggctgc tttcagcgcg
      241 ctcatttccg ccagcgcttc gccggccagg cccttggtca gcgcgccgag cacgcgcacg
      301 tcgagtgcgc ccgccgcgcg cgctttcgac tggatgcgat cgaccactgc cggcgtgtcg
      361 accaccgggt tggtatcggg cggcagcgtc accgtggtga tgccggcggc aagcgcagcc
      421 cgggtttccg aagcgatggt ggctttctgc gttgcgcccg gctcgcgcag gcgcgcggcg
      481 agatcgacga tgcccggcat cagccacagg cccttggcgt cgatcacctc gtcgaaattt
      541 tccttcgtat tttccttgcc gacagtcgct atcgcgcccg cgcggaccgc cacgctggcc
      601 acggcgtcga agccgctgga cggatcgagc acgcgcgcgc cgcggatcag gacggaactc
      661 atgcgcgcac cttcttcggc cgcgatgggc tgcgtgcctg gcccaggcgc gccatcaccg
      721 ccatgcgcac cgcgatgcca tggctcacct gttgcaggat cagcgagcgt tcgctgtagg
      781 cgaggtcgcc ttcgatctcc acgccgcggt tgatcgggcc cggatgcatg atcaccgcat
      841 ctttcttcag ctgcgccacg cgcgcgcgcg tcagcccgta gtcgcggtgg aattcaccga
      901 gcgagggcag gaaggcgccg agcatgcgct ccttctgcag gcgcagcagg atgaccacgt
      961 cggcgccgtc gaggccggcg tcgatcgagc ggaacacctt cacgcccatc tgctcgatgc
     1021 cggcggggat cagcgtgggc ggcgccacca cgcggatttc cttcgtgccg agcgtgcgca
     1081 ggccgtggat gtccgagcgc gccacgcgcg agtgcagcac gtcgccgacg atggcgacgg
     1141 tcagcttcct gaaacactca ttgccagggc ccttggcctt gcggatggtg aacaggtcga
     1201 gcaggccctg ggtcgggtgc gcgtggcggc cgtcgccggc gttgaggatg gcgatgccgg
     1261 gcgccgcgtg ctcggcgaag aaatgcgccg cgccgctggc ttcatggcgg atgacgaaca
     1321 tgtcggcgtg catcgcttcc agcgtcttca gcgtgtcgag cagtgtctcg cccttgctgg
     1381 tgctggaggc ggcgacctgc aggttgagca cgtcggccga caggcgcttg gccgcgagtt
     1441 caaaggtcgt gcgcgtgcgc gtcgacgctt cgaagaacag gttgatgacg gtgcggccgc
     1501 gcaggtgctc gaacttcttc tgcgcgcctt cggctgcgcc ggcgaattgt tccgcgctgt
     1561 cgagcaggct gacgatgagg tcgcggggca gcccttccaa cgtcagcagg tgtttgagat
     1621 gtccgctggc gtcgagttga aggtggggct gcatgctggg cgcgaattat gtcctaccgc
     1681 acccgctaaa tcactgttat tttttattta ttaaaattaa taacaataaa tattttataa
     1741 caagatcagg ggcagtgcgg tggaggctgt caacgtcctt cacggaacca ttgctcgagc
     1801 agcaggcgcg cggcggcgcc gtcgcggtcg cccttgcgca cgcggcgcgc catgcggccg
     1861 tcggcgcgcg cgctgcgcag ctcatcgtcg gccgcgcgcg aggaaaagcg ctcgtcgcac
     1921 aggtgcaccg gcagcttgca gcgctgcccg agttcggcgg cgaagcggcg cg
//
LOCUS       DASZZC010000282         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_276605,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000282 DASZZC010000000
VERSION     DASZZC010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276605"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VHE37_05940"
     CDS             complement(<1..287)
                     /locus_tag="VHE37_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35101.1"
                     /translation="MSAALQHMPGQDRRRELNQFFTPLFVAEALVERYFSHLTSRDLV
                     LEPSCGDGAFLAAIPSHIPAVGVEIDPVMAQRARATGREVISGDFRTVPLT"
     gene            complement(284..904)
                     /locus_tag="VHE37_05945"
     CDS             complement(284..904)
                     /locus_tag="VHE37_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HVT35102.1"
                     /translation="MKRISGTKAVVVQLRPGRGQRKAAGTGARSRSPKTVREHEAHML
                     ASAVYAEMSASNDTRVGALLVKDGNVLSIGCNGTPPGWNNATEHANGRTVEWLCHAEA
                     NAIDKLAAGGGGARGATLYCVYSPCEQCALQIIRARVAQVVYLIPFWKGRGLKWLKKS
                     LIKTVQLDESVLKRVESIAGYQYRRGRECAPVILGACDCAAHRGAA"
     gene            1129..1956
                     /locus_tag="VHE37_05950"
     CDS             1129..1956
                     /locus_tag="VHE37_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT35103.1"
                     /translation="MNILIVDDEPALVAQLEKLTTRLGHNVNTAFNGRQAMESVAAHR
                     DEYHLVISDIRMPEMDGLGLSRTIHKYDVNVPVAFMTGHGDANLAAEAIRTNAIDFVT
                     KPFGIKELRTLLDKISAVYSDSEHQHLWEVDGAHLNCVAVGGPGAIPTIVTELEARVK
                     PYLTAGGIPSRAYALTVAEALRNAMQYGVTDDMGMVMLRVDCNRDVIVTTIVDEGDGF
                     AYESAGLSPTGDDPDKPQRGLFIIRNWMDDVTFGGGGNTIRMVRKLKSPVENVTLAA"
     gene            complement(1940..>2863)
                     /locus_tag="VHE37_05955"
     CDS             complement(1940..>2863)
                     /locus_tag="VHE37_05955"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35104.1"
                     /translation="RFEGIWRISAKVRARMEALDVALVPEGGDELTTLDRNVALVCKA
                     LRMAREDLDRVRKEGGAVALEAALRDLSEAQAALERVGRLSAIGEMAAGVAHEISQPL
                     TTMSMTLDLMSMDEAPRVTVDRGDLSTLQDMVDRIKAIADQLRSVSRQGQDRKREWVA
                     PRLIVEQAMRVLAPHLRLNDVEISVDVPIELPKIHVNPVEMQQVLINLAMNGIDAMAG
                     KSRKSLMVRGLSDAKRITLSVSDLGNGVPQELHEKIFTSFFTTKDPGKGTGLGLSICK
                     RIVDRYGGTIGLVSNSDGSTFVVELPATPRA"
BASE COUNT          560 a          982 c          817 g          504 t
ORIGIN      
        1 atcgtcagcg gcacggtacg gaaatcaccg gagataacct cacgcccggt cgcacgcgcg
       61 cgctgcgcca tcaccggatc aatctcgacg cccaccgccg gaatatgaga agggattgcc
      121 gcgaggaaag ccccgtcgcc gcaggaaggt tccagcacca gatcgcggct cgtcaggtgc
      181 gagaagtagc gctccaccag agcctccgcc acgaacagcg gcgtaaaaaa ctggttgagt
      241 tctcgccgcc gatcctgccc cggcatgtgt tgcaaggcag cgctcacgcc gcgccccggt
      301 gcgccgcgca atcgcacgca cccagaatga ccggcgcgca ctcgcgtccg cggcggtact
      361 ggtatccggc aatgctctcc acccgcttga gcaccgactc gtcgagttgc acggtcttga
      421 tcagactttt cttcagccac ttcaggccgc gtcctttcca gaacggaatg aggtaaacca
      481 cctgagcgac gcgcgcgcgg atgatctgaa gcgcgcactg ctcgcacggg ctgtacacgc
      541 aatacagcgt tgcgccgcgc gcgccaccgc cgcccgccgc aagcttatcg atcgcattag
      601 cctccgcgtg gcacagccac tccacggtcc gcccgttggc gtgctcggtg gcgttgttcc
      661 agcccggcgg ggtgccgttg cagccgatgg acagcacgtt gccgtccttg accagcaaag
      721 cgcccacacg cgtgtcgttc gaagccgaca tctccgcgta caccgctgac gccagcatgt
      781 gagcttcatg ttcgcgtacc gtcttgggag atcgcgagcg cgcgccggtt cccgcagcct
      841 ttcgttggcc acgaccgggg cgcaactgca caacgaccgc cttggttcca gagatgcgtt
      901 tcatcctcat atcgtcctac cactcaacgg acatgcaacc cgccaccact tttagctggc
      961 cgcggcatat acccagccta cctagcgaaa gtgcggccca agagggcaga atggcggaaa
     1021 ccgggccgtc ataaccgggt ttgcgcccgc atcaaaaagc gggatgctgc catcaggcgc
     1081 gtctcctgcg cgctcgccaa ggattggctt tgcgagggga aggatccatt gaacatactg
     1141 attgtcgacg atgagccagc gctggtagct cagctggaaa agctcaccac gcgcctggga
     1201 cacaacgtca acaccgcgtt caatggccgg caggccatgg aaagcgttgc cgcccatcgg
     1261 gacgaatatc acctggtgat cagcgacatt cgcatgcccg agatggacgg actgggcctg
     1321 tcccgcacca tccacaaata cgacgtcaat gtccccgtcg ccttcatgac cggccacggc
     1381 gacgccaatc ttgccgccga ggcgatccgc accaacgcaa tcgatttcgt caccaagccg
     1441 ttcggcatca aagaactgcg gacgctgctc gacaagatct ccgcggtgta ttcggactcc
     1501 gaacatcaac atctttggga ggtcgacggc gctcacctca actgcgtggc cgtcggtggg
     1561 ccgggggcca tccccaccat cgtcaccgag cttgaggcca gggtgaagcc ctacctgact
     1621 gccggcggca ttccgtcacg cgcctacgcc ctgaccgtgg cggaagccct gcgcaatgcc
     1681 atgcagtacg gcgtcaccga cgatatgggg atggtgatgc tgcgcgtcga ctgcaatcgc
     1741 gacgtgatcg tcacgacaat cgtcgacgaa ggagatggat ttgcctacga gagtgccgga
     1801 ctgtccccaa caggcgacga cccggacaag ccgcagcgag gactgttcat catccgcaac
     1861 tggatggacg acgtcacgtt cggagggggc ggtaacacca tccgcatggt gcgcaagctc
     1921 aagagccccg tggaaaacgt cacgctcgcg gcgtagcggg cagctccacc acgaaggttg
     1981 aaccgtcgct attggacacc aggccaatag tgccgccgta gcggtcgacg atccgcttgc
     2041 agatcgatag ccccaggccc gtccccttcc cgggatcctt ggtggtaaag aacgacgtga
     2101 agattttctc gtgcagctct tggggcacgc cattgcccag gtcgctgacc gatagcgtga
     2161 tccgcttggc gtcgctcagc ccgcgcacca tcagcgattt tctcgacttg ccagccattg
     2221 catcgatacc gttcatcgcc agattgatca acacctgctg catttcaacc gggttgacgt
     2281 gaatcttcgg cagctcgatc gggacatcca cagatatctc gacatcattg agccgcaggt
     2341 gcggcgccag cacgcgcatg gcctgctcga cgattaatcg tggcgccacc cactcgcgct
     2401 ttcgatcctg cccctggcgg ctcaccgacc gcaattggtc tgcgatagcc ttgatgcgat
     2461 cgaccatgtc ctgcagtgtg ctcaagtcgc cgcgatctac cgtaacgcga ggcgcctcgt
     2521 ccatcgacat cagatcaagc gtcatcgaca tggtggtcag cggctggcta atctcgtgag
     2581 ccacgcccgc cgccatttcg ccgatcgccg acagccgccc gacgcgctcc agtgcggcct
     2641 gggcttccga caggtcacgc agcgcggcct ccagcgcgac cgcaccgcct tccttgcgta
     2701 cccgatcaag gtcctcacgc gccattcgca gcgctttgca gaccagcgcc acgttccggt
     2761 ccagcgtggt cagctcatca ccgccttcag gcaccaacgc cacatccagg gcttccatgc
     2821 gcgcgcgcac cttggccgag atccgccaga tgccttcaaa tcg
//
LOCUS       DASZZC010000283         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_536996,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000283 DASZZC010000000
VERSION     DASZZC010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536996"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VHE37_05960"
     CDS             complement(<1..708)
                     /locus_tag="VHE37_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HVT35105.1"
                     /translation="MCGIVGAVAQRNVTPILIEGLRRLEYRGYDSTGIALAQDGGGLA
                     LRRSVGKVAKLAEKLAAQPCAGKLGIAHSRWATHGGVTEANAHPHVSGGRIALIHNGI
                     IENYLEIRESLKAKGYAFSSETDTEVIAHLIHHLVQQGRDLTAAVQQAVRQLTGAYAI
                     VVFDAQVPDRLVVARIASPLVVGLGEGENFVASDVQALLPVTRRFYFLEEGDVADVRL
                     DAVTVYDAAGKKVSRKVH"
     gene            complement(857..>1756)
                     /gene="glmU"
                     /locus_tag="VHE37_05965"
     CDS             complement(857..>1756)
                     /gene="glmU"
                     /locus_tag="VHE37_05965"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HVT35106.1"
                     /translation="SAAQRKITEIYSGLLAAPARRLKKWLAKVGNRNAKGEYYLTDVV
                     ALAVKDKVKVAAVSAASADEVEGVNDRAQLARIERAYQQAAAQRLMREGLHIADPARF
                     DLRGTLRHGRDCRIDVGVIVEGDCELGDDVSIGPYTVLRSARLGSGTRVDAHCVIDFA
                     DAGRNCIIGPYARLRPESRLGDGVHIGNFVELKKTTMGDGAKANHLAYLGDAEIGARS
                     NIGAGVITCNYDGAHKHKTTVGADAFVGTDSQLIAPVTVGDRAYIAAGSTISRDAPAD
                     ALTICRAREQRVIPGWKRPQKKK"
BASE COUNT          264 a          605 c          564 g          323 t
ORIGIN      
        1 gtgcaccttg cgcgaaacct ttttcccggc ggcgtcgtac accgtcaccg catccaggcg
       61 cacgtcggcc acgtcgcctt cctcgaggaa atagaaccgg cgcgtcaccg gcagcagcgc
      121 ctgcacgtcg gaggcgacga agttctcgcc ctcgcccagt cccaccacca gcgggctggc
      181 gatgcgcgcg accaccaggc gatcgggcac ctgcgcatcg aacaccacga tggcgtaggc
      241 gccggtgagc tggcgcaccg cctgctgcac ggcggcggtg agatcgcgcc cctgctgcac
      301 caggtggtgg atcaggtgcg cgatgacctc ggtgtcggtc tcggatgaaa acgcgtagcc
      361 cttggccttc aggctttcgc ggatttcaag atagttctcg atgatgccgt tgtggatcag
      421 cgcgatgcgt ccgccggaaa cgtgcggatg cgcattggcc tcggtgacgc cgccatgcgt
      481 ggcccagcgc gagtgcgcga tgccgagctt gcctgcgcag ggctgcgccg ccagcttctc
      541 cgcaagcttc gccaccttgc ccacgctgcg ccgcagcgcc agcccgccgc cgtcctgcgc
      601 cagcgcgatg ccggtggaat cgtagccgcg gtactccagc cgccgcagcc cttcgatcag
      661 gatgggcgtg acgttgcgtt gcgcgaccgc gccgacaatt ccacacatgt tcagatgctc
      721 gcagaggtga aaaataccgc caccgcaccc ctggaattga agttataaat tgttatctga
      781 tttatatttc agacaataat taataacagg caaatggccg gtgcggtgga catattttaa
      841 gccaccccag cgccgctcat ttctttttct gcggccgctt ccagccgggg atgacgcgct
      901 gctcgcgcgc gcggcagatg gtgagcgcgt cggccggcgc gtcgcgggag atggtggagc
      961 cggcggcgat gtaggcgcgg tcgcccaccg tcaccggcgc gatcagctgg ctgtcggtgc
     1021 cgacgaaggc atccgcgccg accgtggtct tgtgcttgtg cgcgccgtcg tagttgcagg
     1081 tgatgacgcc ggcgccgatg ttgctgcgcg cgccgatctc cgcatcgccg agataggcga
     1141 ggtggttggc cttggcgccg tcgcccatcg tggtcttctt cagttcgacg aagttgccga
     1201 tgtgcacgcc atcgccgagc ctgctctccg ggcgcagccg cgcatagggg ccgatgatgc
     1261 agttgcgccc ggcgtcggcg aagtcgatca cgcaatgcgc gtccacccgc gtgcccgaac
     1321 ccaggcgcgc gctgcgcagc accgtgtacg ggccgatgct gacgtcgtcg cccagttcgc
     1381 agtcgccctc gacgatgacg ccgacatcga tgcggcaatc gcgcccgtgc ctgagcgtgc
     1441 cgcgcaggtc gaagcgcgcc gggtcggcaa tgtgcaggcc ttcgcgcatc agccgctgcg
     1501 ccgccgcctg ctggtaggca cgctcgatgc gcgccagctg cgcacggtcg ttcacgcctt
     1561 caacctcgtc ggcgctggcc gcgctcaccg ccgccacctt caccttgtcc ttcaccgcca
     1621 gcgccaccac gtcggtcagg tagtactcgc ccttcgcgtt cctgttgccg actttcgcca
     1681 accatttctt caggcgcctg gccggcgcgg cgagcaggcc ggagtagatc tcggtgattt
     1741 tcctttgcgc tgcgct
//
LOCUS       DASZZC010000284         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_215125,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000284 DASZZC010000000
VERSION     DASZZC010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215125"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VHE37_05970"
     CDS             complement(<1..1082)
                     /locus_tag="VHE37_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HVT35107.1"
                     /translation="MSATKRLNPLDWFFLAAESSESMMHVGSLAPFSPPPDAPKDFLR
                     RLMEEIKDEAHAYPPWSLKLKNPGFIASPLQAWVEDKQFDVEYHVRRSALPSPGDERE
                     LGILVSRLHSNKIDFHRPPWEVHFIEGLEGGRFAIYFKVHHALVDGYTGAKLMERGLS
                     TNPNTRDEPLFYARKPPERVERAAEASAPTLEALMSLAREQAGATRNIAGSFLRILKP
                     AKGKPKTLVAPMSAPRSILNQKISRNRRFATQQFPVERLKRAAKAYDGTLNDIVLALC
                     AHGLRKLLGELGELPPKPLNAMLPVNIRPKDDPGGGNAVGSILATLATDVEDPAERFR
                     AIIASTTAAKAQLSGMTKMGIMEYSAL"
     gene            1173..1316
                     /locus_tag="VHE37_05975"
     CDS             1173..1316
                     /locus_tag="VHE37_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HVT35108.1"
                     /translation="MNIHITLVPVLSIVAGLVILIVPRILNYVIALYLIVAGVVAMVP
                     MPV"
     gene            complement(1320..1850)
                     /locus_tag="VHE37_05980"
     CDS             complement(1320..1850)
                     /locus_tag="VHE37_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT35109.1"
                     /translation="MSITQQQVEARALALTRAFEKAARENSWAWIADEFYAQDCSYLC
                     EYAGTMRVYAGNRDEIRATHYGRDMLVGWEGWSFPIERYTTHGNRIITHWWNRGPGLR
                     ADGTHYQTPGVSFITVGDDGLFTYQLDLFDLAHQMHLCDELEAAGLLSPTLKQNWVIP
                     TKRRLIAQLSRNLPPA"
     gene            complement(1855..2700)
                     /locus_tag="VHE37_05985"
     CDS             complement(1855..2700)
                     /locus_tag="VHE37_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT35110.1"
                     /translation="MSNRIPSLELNQGGPMPQLGLGVWQIADAQVPAAVQAALEAGYR
                     SIDTAAVYGNEAAVGAAIRQSGIARSELYITTKLWNDSQGRERTLRAFDASLKKLDCG
                     YIDLYLIHWPLPRLGLYLETWKTLEELRRDGRVKAIGVSNFQPAHLQRLIDESGTVPA
                     VNQVELHPFLQQRPLRGFHKRHGIVTEAWSPLAQSAALRHPALVKIAARHRRSVAQIV
                     LRWHIEQGIVAIPKSATPARVRENAALFDFQLSPEDLSAISALDQGRRVGPDPDTFAM
                     GAQGR"
     gene            2699..>2963
                     /locus_tag="VHE37_05990"
     CDS             2699..>2963
                     /locus_tag="VHE37_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35111.1"
                     /translation="MSVFRDYERASLAIGALLLSSAAHSAENLPDVVVSATRSAQSAY
                     DIPAAVSGVTVEHELADTPDINLSEYLRDVAGVLVRDRQNYAQD"
BASE COUNT          433 a          972 c         1020 g          538 t
ORIGIN      
        1 agcgcgctgt attccatgat gcccatcttg gtcatgcccg acagctgtgc cttggccgcg
       61 gtggtggagg cgatgatggc gcggaagcgc tcggccggat cttccacatc ggtggccagc
      121 gtggcgagga tggagcccac cgcgttgccg cctccgggat catccttggg ccggatgttc
      181 accggcagca tggcgttgag cggcttgggc ggcagctcgc cgagctcgcc cagcagcttg
      241 cgcaggccgt gcgcgcacag cgcgagcacg atgtcgttga gcgtgccgtc atacgccttg
      301 gctgcgcgct tcaaccgttc caccgggaac tgctgcgtgg cgaagcggcg gttgcgactg
      361 atcttctggt tgaggatgga gcgcggggcc gacatcggcg ccaccagcgt cttcggcttg
      421 cccttggccg gcttgaggat gcgcaggaac gagccggcga tgttgcgcgt ggccccggcc
      481 tgctcgcgcg ccagcgacat cagcgcttcg agcgtgggcg cgctggcctc ggctgcgcgc
      541 tccacgcgct cgggcggctt gcgcgcgtag aacagcggct cgtcgcgggt gttcgggttg
      601 gtcgacagcc cgcgctccat cagcttggcg ccggtgtagc catcgaccag cgcgtggtgc
      661 accttgaagt aaatggcgaa gcggccgccc tcgaggcctt cgatgaaatg cacctcccac
      721 ggcgggcggt ggaagtcgat cttgttgctg tgcaggcgcg acaccaggat gcccagctcg
      781 cgctcgtcgc cgggcgaggg cagcgccgag cgccgcacgt ggtactccac gtcgaactgc
      841 ttgtcttcca cccaggcctg cagcgggctg gcgatgaagc ccgggttctt cagcttgagg
      901 ctccacggcg gataggcgtg cgcctcgtcc ttgatctcct ccatcaaccg gcgcaggaaa
      961 tccttcggcg catcgggcgg cggcgagaac ggggccagcg agccgacatg catcatcgac
     1021 tcgctggact cggcggccag gaaaaaccag tccagcgggt tgaggcgttt ggtggcgctc
     1081 atcgtagttc tcctcgcgtt gggccatggt agcgaagaac tttgcgtacc gcgcggcggc
     1141 ctaagtggca gcacatccgc ggagatgccg tcatgaacat ccacatcacc ctggtgccgg
     1201 tgctgtccat cgtcgccggc ctggtcatcc tgatcgtgcc gcgcatcctc aactacgtga
     1261 tcgcgctgta cctgatcgtc gccggtgtgg tggcgatggt gccgatgccg gtgtaggcct
     1321 caggccggcg gcaggttgcg cgacaactgc gcgatcaacc gccgcttggt cgggatcacc
     1381 cagttctgtt tcagcgtggg cgacagcagt ccggcggctt caagctcgtc gcacaggtgc
     1441 atctggtgcg cgaggtcgaa caggtcgagt tgataggtga acaggccgtc gtcgcccacg
     1501 gtgatgaacg acacgcccgg ggtctggtaa tgcgtgccgt cggcgcgcag accggggccg
     1561 cggttccacc agtgggtgat gatgcgattg ccgtgcgtgg tgtagcgctc gatgggaaag
     1621 ctccagcctt cccagcccac cagcatgtcg cggccgtagt gcgtggcgcg gatctcgtcg
     1681 cggttgcccg cgtacacgcg catggtgccg gcgtactcgc acaggtagct gcagtcctgc
     1741 gcgtagaact cgtcggcgat ccaggcccag gaattctcgc gcgcggcctt ctcgaacgcg
     1801 cgcgtcagcg ccagtgcgcg tgcttccacc tgctgctgcg tgatgctcac cggctcatct
     1861 cccttgtgcg cccatggcga atgtatctgg atcgggcccc acgcgcctgc cctggtcgag
     1921 cgcggagatg gccgacaggt cctcgggaga aagctggaaa tcgaacagcg cggcgttctc
     1981 gcgcacgcgc gccggcgtgg ccgatttcgg aatcgccacg atgccctgct cgatatgcca
     2041 gcgcagcacg atctgcgcca cgctcctgcg gtgccgcgcg gcgatcttca ccagcgccgg
     2101 gtgcctgagc gcggcggact gcgccagcgg gctccaggcc tcggtgacga tgccgtgccg
     2161 cttgtggaag ccgcgcagcg gccgctgctg caggaacggg tgcagttcca cctggttcac
     2221 cgccggcacc gtgccgctct cgtcgatcag ccgctgcagg tgcgccggct ggaagttcga
     2281 cacgccgatg gccttcaccc gtccatcgcg gcgcagttcc tccagcgtct tccaggtttc
     2341 caggtacagc ccgagccgcg gcagcggcca gtggatcagg tagagatcga tgtatccgca
     2401 gtcgagtttt ttcaggctgg cgtcgaatgc gcgcaacgtg cgttcgcggc cctggctgtc
     2461 gttccacagc ttggtggtga tgtacagctc gctgcgcgcg atgccggact ggcggatggc
     2521 cgcgcccacc gcggcctcgt tgccgtagac ggcggcggtg tcgatgctgc ggtagcccgc
     2581 ttccagcgcg gcctgcaccg ccgcaggcac ctgcgcgtcg gcaatctgcc agactcccag
     2641 ccccagctgc ggcatggggc cgccctggtt cagttccaac gaagggatcc tgttgctcat
     2701 gagcgtcttc cgtgactatg agcgcgcaag cttagcaatc ggcgcgctgt tgctgtcgtc
     2761 cgctgcgcac tccgctgaaa atcttcccga cgtggtggtg agcgccacgc gcagcgcgca
     2821 gagcgcgtac gacattcccg cggcggtcag tggcgtgacg gtggagcatg agctggccga
     2881 caccccggac atcaacctgt ccgaatatct gcgcgacgtg gccggcgtgc tggtgcgcga
     2941 tcgccagaac tacgcgcagg acg
//
LOCUS       DASZZC010000285         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_341206,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000285 DASZZC010000000
VERSION     DASZZC010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341206"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /gene="rfaE2"
                     /locus_tag="VHE37_05995"
     CDS             <1..969
                     /gene="rfaE2"
                     /locus_tag="VHE37_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HVT35112.1"
                     /translation="YGKGSLADVAHLISAARAAGKPVLIDPKGSDYRPYQGATLLTPN
                     MSEFEAVVGRCRDEADIVAKGENLRKALKLDALLVTRSEHGMTLIQPGQAPLHLPTEA
                     REVFDVTGAGDTVIATLGAALAAGQDFAHACRLANIAAGIVVGKIGTATASVEELQRA
                     IRREHEDDSGVLSEDALLARVKEARARGETIVMTNGVFDILHSGHVRYLEAAKKLGDV
                     LIVAVNSDASVKKLKGPTRPINALADRMKLLAALKCVDWVVPFSEETPARLIGRVLPS
                     LLVKGGDYQPQDIAGYDAVTGNGGQVLVLDFHKGYSTTRIIEKARG"
     gene            1224..>1867
                     /locus_tag="VHE37_06000"
     CDS             1224..>1867
                     /locus_tag="VHE37_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-glutamate deacylase"
                     /protein_id="HVT35113.1"
                     /translation="MDGAKPGWDILIKNALVFDGTGAAGVVQDVAIAGERVAAMGPGL
                     ADDHARRVIDAQGQWLIPGMLDIHTHFDLEVELEPGLPEAVRHGTTTVVVSNCSLGVA
                     FGAQRVNGADPIVDCFARVENVPKHVLKKVADSVTWNDSKDYLDHFEKIPLGPNIVPM
                     IPHSMLRIEVMGLNASVVRDPTHAELARMQELVEKGMREGYVGFSTDALPFHFLA"
BASE COUNT          338 a          591 c          641 g          297 t
ORIGIN      
        1 tacggcaagg gttcgctggc cgatgtggcg catttgatct cggccgcgcg cgcggccggg
       61 aagccggtcc tgatcgatcc caagggcagc gactaccggc cataccaggg cgccacgctg
      121 ctgacgccga acatgagcga attcgaggcg gtggtcggtc gctgccgcga cgaggcggac
      181 atcgttgcca agggcgagaa cctgcgcaag gcgctcaagc tcgacgcgct gctggtgacg
      241 cgcagcgagc acggcatgac gctgatccag ccgggccagg cgccgctgca tttaccgacc
      301 gaggcgcgcg aagtgttcga cgtgaccggc gccggcgaca ccgtcatcgc cacgctcggc
      361 gcggcgctgg cggcggggca ggacttcgcc catgcctgcc gcctggccaa catcgccgcc
      421 ggaattgtcg tgggcaagat cggcaccgcc accgcgtcgg tggaggaatt gcagcgcgcc
      481 atccgccgcg agcacgagga tgacagcggc gtgctcagcg aagacgcgct gctggcgcgc
      541 gtgaaggaag cccgggcgcg cggcgaaacc atcgtcatga ccaacggcgt gttcgacatc
      601 ctgcacagcg gccacgtgcg ctatctcgaa gcagcgaaaa aactcggcga cgtgctgatc
      661 gtggcggtga acagcgacgc ctcggtgaag aagctcaagg gcccgacgcg cccgatcaac
      721 gcgctggcgg accggatgaa actgctggcc gcgctcaagt gcgtggattg ggtggtgccg
      781 ttctccgagg aaacgccggc gcgcctgatc ggccgcgtgc tgccgagcct gctggtcaag
      841 ggcggcgact accagccgca ggacatcgcc ggctacgacg ccgtcaccgg caacggcggc
      901 caggtgctgg tgctcgattt ccacaagggc tactcgacca cgcgcatcat cgagaaggcg
      961 cgcggctgag agggcttgtg cgccatgccg gcgcgaagcg cgcgcaaggc gcgctcccaa
     1021 gaaaaaatgc gcaggcgttt tttgcggaaa tggcctgaac cgcaccgccg gtttttgtgt
     1081 tattaattat agtattaaag taaaagcagt acatctttta taacaggtca aatgccggtg
     1141 cggtggaggc ggatttcgct ctcgtcgctg gccagatcgt gtcgtagact cgcgccaact
     1201 tctttcttct ctcaaggtgc gacatggacg gcgccaagcc cggctgggac atcctgatca
     1261 agaacgcgct ggtgttcgac ggcaccggcg ctgcgggcgt ggtgcaggac gtggccatcg
     1321 ccggcgagcg cgtggcggcg atggggccgg ggctggcgga cgaccatgcg cgccgcgtga
     1381 tcgacgcgca gggccagtgg ctgattcccg gcatgctcga catccacacg catttcgatc
     1441 tcgaggtgga actcgaaccg ggtctgcccg aggccgtgcg ccacggcacc accaccgtgg
     1501 tggtgtccaa ctgctcgctc ggcgtggctt tcggcgcgca gcgcgtcaac ggcgccgacc
     1561 cgatcgtcga ctgcttcgcg cgcgtggaaa acgtgcccaa gcacgtgctg aagaaagtcg
     1621 ccgacagcgt gacctggaac gacagcaagg actatctcga ccacttcgag aagatcccgc
     1681 tgggcccgaa catcgtgccg atgattccgc actccatgct gcgcatcgag gtgatgggcc
     1741 tcaacgcctc ggtggtgcgc gaccccacgc acgcggagct ggcgcgcatg caggagctgg
     1801 tggagaaggg tatgcgcgag ggctatgtcg gcttctcaac cgacgcgctg ccgtttcact
     1861 tcctggc
//
LOCUS       DASZZC010000286         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_599678,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000286 DASZZC010000000
VERSION     DASZZC010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599678"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHE37_06005"
     CDS             <1..315
                     /locus_tag="VHE37_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HVT35114.1"
                     /translation="ARDKEADAMSVTVRIMGKEYTVACPKEEHEALVKSADYLNERMT
                     TIRKRGKALGADKIAVMAALNIARELLELRGVEGVASANQQSIERLRQLKLDIDSTLA
                     LE"
     gene            392..577
                     /gene="ssrS"
                     /locus_tag="VHE37_06010"
     ncRNA           392..577
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VHE37_06010"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            597..1157
                     /locus_tag="VHE37_06015"
     CDS             597..1157
                     /locus_tag="VHE37_06015"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HVT35115.1"
                     /translation="MREQLRRRRAQLAPRERIKAAQRAASHLMKTRWMAQARHVAVYL
                     AAGSELDTLVLIERLWRAGKRVYVPRVRAGGHLTFHTYTPCTPLRANAHGIAEPSDGT
                     PRRQVRGMDLVIVPLTAFDPRGNRLGSGAGYYDRTLAARRSGSRPRLLGYAYALQEVP
                     ALPAEYWDVRLDAVITEKGVRRWRTG"
     gene            1142..1612
                     /locus_tag="VHE37_06020"
     CDS             1142..1612
                     /locus_tag="VHE37_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HVT35116.1"
                     /translation="MAYWLMKSEPDCFSIDHLKARPKQTAPWDGVRNFQVRNMFRDDM
                     KQGDTAFFYHSSCDVPGVYGVMKIVSNAYPDPTQFDPKADHYDPTAKPEEPRWLLVDV
                     KFERKLKAPVTLATLRAHAAKLKGLRLLEPGSRLSVVPVSPAHWNYILSLEPKA"
     gene            1794..3053
                     /locus_tag="VHE37_06025"
     CDS             1794..3053
                     /locus_tag="VHE37_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HVT35117.1"
                     /translation="MRNWFRKVSEHPTVKQLGPGLITGAADDDPSGIATYSQAGAQFG
                     NDMLWTVVFTYPLMVGIQMVSARLGYVTGRGLAANIRSVFPRWLLYGIVGLLLFANTL
                     NIAADVAAMGQALQLLLGGSHHAYSLMFGLLCLTLQVFLPYQRYVRYLRWLTLALLSY
                     VAVVFFIDVPWFDVLKDALLPETAFTSAGIVMIVAVFGTTISPYLFFWQAAQEVEDLR
                     GRKGASASDDVPAHLRRIKTDTWFGMGFSSLIAFFIILTTAATLHAHGVQHIETSAQA
                     AEALRPIAGDFTFLMFALGIIGTGMLAVPVLAGSAAYAVTESFEWRNGLDLKLLEARE
                     FYGIIAFATLGGVVLGFTSIDPIQALIASALVNGVIAVPIMVVMMLLASRPDVLGKYV
                     ITRRHRVGGWAATAMMAAAVVAMFATL"
     gene            complement(3075..3677)
                     /locus_tag="VHE37_06030"
     CDS             complement(3075..3677)
                     /locus_tag="VHE37_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HVT35118.1"
                     /translation="MDIPRTGSTSLKLQLGQSLGQTYAKSDLRIGERHSAVRDHCTAL
                     EMRELLGAERWNRLYRFSFVRDPWDRMVSFYLYRRDVARNLPPSVPFDDYVRRLRGPV
                     PAQPKPRSPFFRVVFHRSCSQYLYDADGQLLVNFVGHYERRAQDLALLAEARGLIVDP
                     SLSAYATAAQSSYRSWYTPETAQIVGEFFADDLRNFGYSF"
     gene            complement(3837..>4797)
                     /gene="ilvA"
                     /locus_tag="VHE37_06035"
     CDS             complement(3837..>4797)
                     /gene="ilvA"
                     /locus_tag="VHE37_06035"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HVT35119.1"
                     /translation="AAYIKALSPKTKIIAVEPDDSNCFAAALAAGRRVVLPQVGLFAD
                     GVAVKQIGEENFRVARHLVDGVVLVSVDEICAATRDVFYENRCLPEPAGALAVAGLKK
                     YVAEHRPAGKTMAAIVSGANVNFDRLRHIAERAELGDGHESLLAVTIPEQPGSFKRFL
                     RHMGKRGITEFNYRYATPSEAHIFVGVKLDAGESRDAVIRTLRDDGYPVVDMSNNEMA
                     KVHVRYMVGGRCPGLPHERLLRFEFPERPGACLEFLNAVGQRWNISLFHYRNHGAANG
                     RVLCGLQIPRGEWTAAKESLDALGYQWWEETGNAAYALFLGTN"
BASE COUNT          794 a         1540 c         1602 g          861 t
ORIGIN      
        1 gcgcgcgaca aggaggccga tgccatgtcg gtcaccgtgc gcatcatggg caaggaatac
       61 accgtggcct gcccgaagga agagcatgag gcgctggtca agtcggccga ttatctcaac
      121 gagcgcatga ccaccatccg caagcgcggc aaggcgctgg gcgcggacaa gatcgcggtg
      181 atggcggcgc tgaacatcgc gcgcgagctg ctcgaactgc gcggcgtgga aggcgtggca
      241 tccgccaacc agcagtcgat cgaacggttg cgccagctca agctcgacat cgactcgacg
      301 cttgcgctgg aatagttcgc cgtcaagcga aatttttccg cggacgaatg gcgctggtgc
      361 gcgcagcctc gcgtgatatt ctcctccccg gtgtctctcg tggtgttcgg aatgcggttc
      421 caaacccctt gagcctaatt gctaacccag ggaaccttac ccgtgcctgg cgtgcaggcc
      481 caccttgcag tgggaagccc gatggcatgg cgcggtgccc acttgagacc acagtctcaa
      541 ggtgacggat cagcatgacg acacgcgcgg gagacaccac taacaccaag cctcaattgc
      601 gtgagcaatt gaggcgccgc cgggcgcaac tggcgccgcg cgagcgtatc aaggccgcgc
      661 agcgcgccgc ctcccatctg atgaagacgc gctggatggc gcaggcgcgg cacgtcgccg
      721 tgtacctcgc cgccggcagc gaactggaca cgctggtgct gatcgagcgc ctgtggcgcg
      781 ccggcaagcg cgtgtacgtg ccgcgcgtgc gcgccggcgg gcacctgacc tttcacacct
      841 acacgccgtg cacgccgctg cgcgccaatg cgcatggcat cgccgaaccg tccgacggca
      901 cgccgcggcg gcaggtgcgc ggcatggacc tggtgatcgt gccgctcacc gcatttgacc
      961 cgcgcggcaa tcgcctgggc agcggcgccg gttactacga ccgcacgctc gccgcgcggc
     1021 gcagcggttc gcgcccgcgc ctgctcggct acgcttacgc gctgcaggaa gtgccggcgc
     1081 tgccagcgga atactgggac gtgcggctgg acgcggtgat cacggaaaaa ggagtgcggc
     1141 gatggcgtac tggctgatga agagcgagcc cgattgcttc tcgatcgatc atctcaaggc
     1201 gcggccgaag cagactgcgc cgtgggacgg cgtgcgcaac ttccaggtgc gcaacatgtt
     1261 ccgcgacgac atgaagcagg gagataccgc gtttttctat cactcgtcgt gcgacgtgcc
     1321 cggcgtgtac ggcgtgatga agattgtcag caatgcgtat cccgatccga cgcagttcga
     1381 tccgaaggct gatcattacg atccgaccgc caagccagag gagccgcgct ggctgctggt
     1441 cgacgtgaag ttcgaacgca agctgaaggc gccggttacg ctggccacgc tgcgcgccca
     1501 tgcggcgaag ctcaagggat tgcgcctgct cgaaccgggc agccgcctgt cggtggtgcc
     1561 ggtttcgccg gcgcactgga attacatcct gtcgcttgaa cccaaggcgt agatcggcgg
     1621 tccctaagcc gcgcgcgggc tttaccgcat gccgtttttc cttgttatca attatcatct
     1681 gattttataa tcagtctata atttatctca ttgattccgg caatgcggtg cggcagtttt
     1741 ccgcacgatt ttcggcggtg cggcagtgac aacagacaac gacggtcagc aacgtgcgca
     1801 actggttccg caaagtcagc gagcatccca cggtgaaaca gcttggcccc gggctgatca
     1861 ccggcgcggc tgacgatgat ccttccggca tcgccaccta ttcccaggcc ggcgcgcagt
     1921 tcggcaacga catgctgtgg acggtggtgt tcacctatcc gctcatggtc ggcatccaga
     1981 tggtgtcagc ccgcctgggc tacgtcaccg ggcgcgggct ggcggcgaac atccgctcgg
     2041 tgtttccgcg ctggctgctg tacggcatcg tcggcctgct gctgttcgcc aacacgctca
     2101 acatcgcggc cgacgttgct gccatgggcc aggcgctgca gctgctgctt ggcggctcgc
     2161 accatgccta ttcactgatg ttcggcctgc tgtgcctgac gctgcaggtg ttcctgccgt
     2221 accagcgcta cgtgcgctac ctgcgctggc tcacgctggc gctgctgtca tacgtggcgg
     2281 tggtgttctt catcgacgtg ccctggttcg acgtgctgaa agacgcgctg ctgccggaga
     2341 ctgctttcac cagcgcgggc atcgtgatga tcgttgccgt gttcggcacc accatcagcc
     2401 cctacctgtt cttctggcag gcggctcagg aagtggagga tctgcgcgga cgcaagggcg
     2461 catccgccag cgacgacgtg ccggcccacc tgcgccggat caagaccgac acctggttcg
     2521 gcatgggctt ttccagcctg atcgcgttct tcatcatcct caccaccgcg gccacgctgc
     2581 atgcgcatgg cgtgcagcac atcgaaacat cagcgcaggc agccgaagcg ctgcgcccca
     2641 tcgccggcga tttcaccttc ctgatgttcg cgctcggcat catcggcacc ggcatgctgg
     2701 cagtgccggt gctggccggc tcggcggcgt acgcggtgac cgagagtttc gagtggagga
     2761 acgggctcga cctcaagctg ctcgaagcgc gcgagttcta cggcatcatc gccttcgcca
     2821 cgctcggcgg cgtggtgctg ggcttcacca gcatcgaccc gatccaggcg ctgatcgcct
     2881 ccgcgctggt caacggcgtg atcgcggtgc cgatcatggt ggtgatgatg ctgctggcct
     2941 cgcggccgga cgtactgggc aaatatgtga tcacgcgtcg gcaccgcgtc ggcggttggg
     3001 ctgccaccgc gatgatggcg gcggcggtgg tggcgatgtt cgcgacgctc tagcggcgcg
     3061 accggcgccg ggcttcagaa cgagtagccg aagttgcgca ggtcatcggc aaagaattcg
     3121 ccgacgatct gcgccgtctc gggcgtgtac cagctgcggt aactggattg cgcggcggtg
     3181 gcataggcgg acaaggatgg gtcgacgatc agtccgcggg cctccgccag cagcgcgaga
     3241 tcctgggcgc gccgctcgta gtggccgacg aagttgacca gcagttggcc atcggcgtcg
     3301 tatagatatt gactgcacga gcggtgaaaa accacgcgga agaacggcga gcgcggtttt
     3361 ggctgcgccg gcaccggccc gcgcagcctg cgcacgtagt cgtcgaaggg caccgacggc
     3421 ggcaggttgc gtgcaacgtc acggcggtag aggtaaaacg agaccatgcg atcccacggg
     3481 tcgcgcacga acgagaaccg gtacagcctg ttccacctct cggcgcccag cagctcccgc
     3541 atctccagtg cggtgcaatg gtcgcgcacg gccgagtggc gctcgccgat gcgcagatcg
     3601 gacttggcgt aggtctggcc caggctctgt cccagctgca gcttgaggct ggtgctgccg
     3661 gtgcggggaa tgtccacaaa ccagagcccc gcttcggtga tctgcgcaat gacgtgcgct
     3721 tcctgcgcgg gatcggtcag tcgcagatgc cgtttgcctg ggttggctgc agcagccggc
     3781 tttccggggg ggcttgtcca tcatctgctg gatcggtggt gcggcgccgg tgcgcttcag
     3841 ttcgtgccga ggaacagcgc ataggctgcg ttgccggttt cttcccacca ctgatagccg
     3901 agcgcgtcga gcgattcctt tgccgccgtc cactcgccgc gcgggatctg cagcccgcac
     3961 agcacgcggc cgttggcggc gccatgattg cggtagtgga acagcgagat gttccagcgc
     4021 tggcccaccg cgttgaggaa ttccaggcag gcgccggggc gctcgggaaa ctcgaagcgc
     4081 agcagccgct cgtggggcag cccaggacag cggccgccga ccatgtagcg cacgtgcacc
     4141 ttggccatct cgttgttcga catgtcgacc accgggtagc cgtcgtcgcg cagcgtgcgg
     4201 atcaccgcgt cgcggctctc gccggcgtcg agcttcacgc cgacgaagat gtgcgcctcg
     4261 ctcggcgtgg cgtagcggta attgaactcg gtgatgccgc gcttgcccat gtgcctgagg
     4321 aaacgcttga agctgccggg ctgctcggga atggtcaccg ccagcagcga ttcgtggccg
     4381 tcgcccagtt cggcacgctc ggcgatgtgg cgcaggcggt cgaagttgac gttggcgccg
     4441 gacacgatcg ccgccatggt cttgccggcg ggacggtgct cggccacgta tttcttcagg
     4501 ccggccacgg ccagcgcgcc ggcgggctcg ggcaggcagc ggttttcgta gaacacgtcg
     4561 cgcgtggcgg cgcagatctc gtccacgctg accagcacca cgccgtcgac caggtgtcgc
     4621 gcgacacgga aattctcttc gccgatctgc ttcaccgcca cgccgtcggc aaacaggccc
     4681 acctgcggca gcaccacgcg gcggccggcg gcgagcgcgg cggcgaagca gttggagtcg
     4741 tccggttcga ccgcgatgat tttcgttttc ggcgacagcg ccttgatata ggcggca
//
LOCUS       DASZZC010000287         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_277018,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000287 DASZZC010000000
VERSION     DASZZC010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277018"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VHE37_06040"
     CDS             <1..1406
                     /locus_tag="VHE37_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35120.1"
                     /translation="LQRQYAFTPDGLTNHWTNEFADRSAAMAAISDADALAYIRQDNY
                     TPLLQAMQQPMAYYGWRPDLDLEQGFDAEGYARDGSWWRAFRYKPFLGTFWPTNGSTD
                     DVIIRLPAAYYTGSDGRPSRAIYKINLAIAEAAMTVSDAVADADVVRTVEPVSEDLAG
                     MDLDGDGHIGGMVTTIRGLPAHYVANLGTAGSPQYPPVLRWQYPAGTEFLHTVRYIDP
                     DAPDLLSVRLKEVRYALKFATLSSGDYNFIYGEEARDASNGLPPSFGGTALTGLNNNF
                     GWLLDAFIEDEQGRLRLQTYEEHFYCLGCHSGIGVTADFTFGFPRKVPGALGWGHQSI
                     AGMQDVAQAGQLDPEILTYFRRVGGGDEFRSNDEISARFFPGGTLDEATVRRAAPGGD
                     QDLAFLLAPSRTRALALDKAYMALVREQSFVHGRDAVLAPRTNVYPLIAGNGDTGLSS
                     GVGVFDDGRIWLAWPAP"
     gene            complement(1425..2006)
                     /locus_tag="VHE37_06045"
     CDS             complement(1425..2006)
                     /locus_tag="VHE37_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35121.1"
                     /translation="MALRLIAGLAALLLTVAQAQNEAYLTRSQVEQCLRAKARAGDNQ
                     QMLLNEKARLDAVSLQFQAEAGRLRGRYAQIEARRRQQDHLEAATGGLRTGRSMGDAD
                     LAAIDRELSDIERGRAELEQQRADYNHALDDHNARFARHYAQVQELNRRFDAQRSYAQ
                     TVDTRCTHALVRESDRRAAQAVVDAENARRPRR"
     gene            complement(1991..>2445)
                     /locus_tag="VHE37_06050"
     CDS             complement(1991..>2445)
                     /locus_tag="VHE37_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVT35122.1"
                     /translation="HEAELVVLLTGGGSDIPEHVALDYVAGITLGLDLTLRELQTTLK
                     EKGRPWELAKSFDCAAPLGDIKPYIEQELQDLQFTCRVNGEVRQRGDTRQMTYSVARQ
                     IHLLSQTWALLPGDVIYTGTPAGVGPLVAGDVVQLEGPAIGRFEWRCD"
BASE COUNT          384 a          821 c          805 g          435 t
ORIGIN      
        1 acctgcagcg ccagtacgca tttacgccgg acgggctgac caaccactgg accaacgaat
       61 tcgccgatcg cagcgcggcg atggcggcga tcagcgatgc cgatgcgctt gcctacatca
      121 ggcaggacaa ctacacgccg ctgctgcagg ccatgcagca acccatggcc tactacggct
      181 ggcgcccgga tcttgatctg gaacaaggct tcgacgccga gggatacgcc cgcgatgggt
      241 cgtggtggcg cgcgttccgc tacaagccgt ttctcggcac gttctggccc accaatggat
      301 cgaccgacga tgtgatcatc cggctgcctg ccgcctacta cacaggatcg gatggccggc
      361 cttcacgtgc gatctacaag atcaacctgg ccatcgccga ggccgccatg acggtgtccg
      421 atgctgtcgc cgatgcggac gtggtccgca cggtcgagcc ggtgagcgag gatctagccg
      481 ggatggacct ggatggcgat ggccatatcg gcggaatggt gacgaccata cgcggcctgc
      541 cggcgcatta cgtggccaat ctcgggactg cgggcagccc gcaatatcca ccggtgctgc
      601 gctggcaata tccggccggc accgagttcc tgcataccgt gcgctacatc gaccccgatg
      661 cgccggacct gctgtcggtc cgcttgaagg aagtgcgtta cgcgctcaaa ttcgcgacct
      721 tgtccagcgg cgattacaac ttcatctacg gcgaagaggc gcgcgacgcg tccaacggcc
      781 tgccgccgtc gttcggcgga actgcgctta ccgggctgaa caacaacttc ggctggctgc
      841 tggacgcttt catcgaagac gagcagggca ggttgcgcct gcagacctat gaagagcact
      901 tctactgctt ggggtgccac agcggtatcg gcgtgacggc ggacttcact ttcggctttc
      961 cgcgcaaggt ccccggcgca ttgggttggg gacaccagtc cattgccggg atgcaggatg
     1021 tggcgcaggc ggggcagttg gatcccgaaa tcctcaccta tttccggcgc gtgggcggcg
     1081 gcgacgagtt ccgctcgaac gacgagatca gcgcgcgctt ctttccggga ggaacgctgg
     1141 acgaagcgac ggtgcgccgt gccgcgcccg gcggcgacca ggacctggcg ttcctgctcg
     1201 cgccgtcgcg cacccgcgcg ctggcgctgg acaaggccta catggcgctg gtgcgcgagc
     1261 agagctttgt ccatggccgt gacgccgtat tggcgccgcg caccaacgtc tacccgctga
     1321 ttgcaggcaa cggcgacacg gggctcagtt cgggcgtcgg cgtgttcgat gacggccgca
     1381 tctggctcgc ctggccagcg ccgtagagga agaccatcca gagcctagcg gcgcggccgg
     1441 cgcgcatttt cggcgtcgac cacggcctgc gccgcccgcc ggtcactctc gcgcaccagc
     1501 gcgtgcgtgc agcgcgtatc caccgtctgt gcgtagctgc gctgtgcatc gaagcgccgg
     1561 ttcagttcct gcacctgcgc gtaatggcgc gcgaagcggg cgttgtgatc gtccagtgcg
     1621 tggttgtaat cggcgcgctg ttgctcgagt tcggcgcggc cgcgctcgat atctgaaagt
     1681 tcacggtcga tcgctgccag gtcggcatcg cccatcgaac ggccggtgcg caggccgccg
     1741 gtggccgcct ctagatggtc ctgctggcgc cgtcgcgcct cgatctgtgc gtagcgcccg
     1801 cgcagccggc ctgcttccgc ctggaactgc agcgacacgg catcgagccg cgccttttcg
     1861 ttcagcagca tttgctggtt gtcgcccgca cgcgccttcg ccctcaggca ttgctccacc
     1921 tggctgcgcg tcaggtacgc ctcgttctgg gcctgcgcca cggtcaacag cagcgcggcc
     1981 aggcccgcga ttagtcgcag cgccattcga aacggccgat ggcgggtcct tccagttgca
     2041 ccacgtcgcc cgcgaccagc gggccgacgc cagcgggcgt gccggtgtaa atcacgtcgc
     2101 ccggcagcag cgcccaggtc tgcgacagca ggtgaatctg ccgcgccacg ctgtaggtca
     2161 tctggcgcgt gtcgccgcgc tggcgcacct cgccgttgac ccggcaggtg aactgcaggt
     2221 cctgcaactc ctgctcgatg tacggcttga tgtcgcccaa cggcgcggcg cagtcgaacg
     2281 acttggccag ttcccacggc cggccctttt ccttgagcgt ggtctgcagt tcgcgcagcg
     2341 tgagatcgag gccgagcgtg atgccggcga cgtagtcgag cgccacatgc tcgggtatgt
     2401 cgctgccacc gccggtgagc agcaccacca gttccgcctc gtgat
//
LOCUS       DASZZC010000288         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1181022,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000288 DASZZC010000000
VERSION     DASZZC010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181022"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VHE37_06055"
     CDS             complement(<1..785)
                     /locus_tag="VHE37_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVT35123.1"
                     /translation="MGTESRGVALLLAAFAIGIAWRALTPAQAPAVVVVPAPAQAPPQ
                     RVLLAPAAIDATLAQHSTVMEREAALAGGREVSIDTQTHAVIRVGPAGPAHADQPVSE
                     EAHKALMARLDTRMTPLMDAARAGDAAQVQQLLKTAPGDLNVHDRYGNTALMYASRAH
                     HITAVQYLLDAGADPRMRNEQGANAWFWTLVDAETAPPAAEDQRVAILDQLRGHGADV
                     NDADANGATLLHRAASLDDLPVVEFLLAHDANLEARDHQGRTPL"
     gene            complement(775..1293)
                     /locus_tag="VHE37_06060"
     CDS             complement(775..1293)
                     /locus_tag="VHE37_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVT35124.1"
                     /translation="MFRTVAVLGLLLAAQAHAGVGVYKWVDAEGKVHFTDQPTGNQPV
                     QQVNTSTLPLGLQDRLRSLDHNLSITHFDGDTTIGIVCAEFDTDYVTSEPVFAQAVEA
                     AKLGTVKTTSSYYDSTRSSYYWQAPARQSYDPRKCPRSSSKKRTLQSRMYEIRFNPDA
                     VSVYRSEESHGH"
     gene            complement(1483..>1724)
                     /locus_tag="VHE37_06065"
     CDS             complement(1483..>1724)
                     /locus_tag="VHE37_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HVT35125.1"
                     /translation="PRCIVIRLARVPFMDITGLQTLEEAIDNLQRRKVRVILCEANQR
                     VLLKLTRAHIVRRDEHPPRYFRSLADAVAAAGNQK"
BASE COUNT          256 a          577 c          576 g          315 t
ORIGIN      
        1 agcggcgtgc ggccttggtg gtcgcgcgcc tccagattgg catcgtgcgc cagcaggaac
       61 tcgaccaccg gcaggtcatc gaggctggcg gcgcggtgca acagcgtggc gccattggcg
      121 tccgcgtcgt tgacatccgc cccatggccg cgcaactggt cgaggatggc cacgcgctgg
      181 tcttcggccg ccggcggcgc ggtttccgca tcgaccagcg tccagaacca ggcattggca
      241 ccctgctcgt tgcgcatgcg cggatcggcg ccggcgtcga gcagatactg caccgcggtg
      301 atgtggtgcg cgcggctggc gtacatcagc gcggtgttgc cgtagcggtc atgcacgttg
      361 agatcgcccg gtgcggtctt cagcagctgc tgcacctgcg ccgcgtcgcc ggcacgcgcg
      421 gcgtccatca gcggcgtcat gcgggtgtcc agccgcgcca tcagcgcctt gtgtgcctcc
      481 tcgctcaccg gctggtcggc atgcgccggt cctgccggcc ccacgcggat gaccgcgtgc
      541 gtctgcgtgt cgatcgacac ctcgcggccc ccggccagcg ccgcttcgcg ctccatcacc
      601 gtgctgtgct gcgccagcgt ggcgtcgatg gccgccggcg ccagcagcac gcgctgcggc
      661 ggcgcctgcg caggcgccgg caccaccacg accgcgggag cctgcgccgg cgtcagcgcg
      721 cgccaggcga tgccgatggc gaatgccgcc agcagcaagg caacgccgcg cgactcagtg
      781 cccatgcgac tcttcactgc ggtacacgct caccgcatcg ggattgaagc ggatttcgta
      841 catgcgcgat tgcagcgtgc gcttcttgct gctggagcgc gggcatttgc gcgggtcata
      901 ggactgccgc gccggcgcct gccagtagta gctggaacgg gtcgaatcgt aataggacga
      961 agtggtcttc accgtgccca gcttggcggc ttccaccgcc tgcgcgaaaa ccggctcgct
     1021 ggtgacgtaa tcggtgtcga actcggcgca gacgatgccg atggtggtgt cgccatcgaa
     1081 atgcgtgatc gacaggttgt gatccaggct gcgcagccgg tcctgcaggc ccagcggcaa
     1141 ggtcgaggta ttgacctgct gcaccggctg gttgcccgtg ggctggtcgg tgaaatgcac
     1201 cttgccctcg gcatcgaccc acttgtagac gccaaccccg gcgtgggcct gcgccgccag
     1261 cagtaggccg agcacggcca cggttctaaa catgcacatc aggatttccc ccccaagaaa
     1321 tcggctctga ttttagccgc tcgcaggcat gccgttgcga cgctccgatg ttttttccat
     1381 ggctggcgcc ctaccgcacc ccgctgccgc gtgttactaa atatttagta ttttacttat
     1441 tatgcatctt tttgtaacag acggaatgcg ggtgcggtca agctatttct ggttgcccgc
     1501 ggccgccacc gcatcggcca ggctgcggaa gtagcgcggc ggatgctcgt cacggcgcac
     1561 gatgtgcgcg cgcgtgagct tgagcagcac gcgctggttg gcctcgcaca ggatcacccg
     1621 caccttgcgc cgctgcaggt tgtcgatggc ctcttccagc gtctgcaggc cggtgatgtc
     1681 catgaacggc acgcgcgcca ggcggatgac gatgcagcgc ggat
//
LOCUS       DASZZC010000289         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_600004,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000289 DASZZC010000000
VERSION     DASZZC010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600004"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1176)
                     /locus_tag="VHE37_06070"
     CDS             complement(181..1176)
                     /locus_tag="VHE37_06070"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HVT35126.1"
                     /translation="MRSEYEYGPKRVLVTGGAGFIGSHLCERLVAAGHEVLCVDNFYT
                     GSRRNVLELLDHPSFELMRHDVTFPLYVEVEEIYNLACPASPIHYQFDPVQTTKTSVH
                     GAINMLGLAKRCRAKIFQASTSEVYGDPAVHPQPEEYWGNVNPIGPRSCYDEGKRCAE
                     TLFFDYHRQHKLRIKVARIFNTYGPHMHPNDGRVVSNFIVQALKGEDITLYGDGMQTR
                     AFCYVDDQLDGWLRLMDHTPDDFTGPVNIGNPVEIPVKEMAARIVKLVNGKSKIVHTQ
                     PLPVDDPLQRCPDITLARKELKWEPKVDLDTGLGRTIEYFDTLLKSNKGIRGIGR"
     gene            1434..3425
                     /gene="tkt"
                     /locus_tag="VHE37_06075"
     CDS             1434..3425
                     /gene="tkt"
                     /locus_tag="VHE37_06075"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVT35127.1"
                     /translation="MPNRLELANAIRALAMDAVQKANSGHPGMPMGMADIAEVLWNDF
                     LKHNPADPKWPDRDRFVLSNGHGSMLLYALLHLTGYDLPMSELQNFRQLHSKTAGHPE
                     YGITPGVETTTGPLGQGLANAVGFALAERLLAARYNRDGHDIVDHYTYVFTGDGCLME
                     GISHEACSLAGTLGLNKLIVLYDDNNISIDGEVEGWFRDDTPARFEAYGWQVIRNVNG
                     HDARDVKKAIETARASADKPTLICCKTIIGYGAPTLQGKEKCHGAPLGDAEITLARTQ
                     LKWPHAPFTVPADVAAGWNARERGASAQRAWQEKFQAYAAKFPAEAAEFSRRMKGELP
                     SEWKTAIDAVIAQLQKADKPDASRKSSKAVLNIIAPALPELLGGSADLSESNCTDFKG
                     YLPLRHGKLAGNYINYGVREFGMSATLNGLALHGGFIPFGGTFLTFSDYARNAIRMAA
                     LMKIGSIFVYTHDSIGLGEDGPTHQSVEHAASLRLIPNVEFWRPGDAFETAIAWKAAV
                     ERRNGPSLLALSRQNLNAQAHPDAHTAAAARGGYVLWEPATAPQAVILATGSEVQLAV
                     DAAKKLAGKLAVRVVSLPCVERFMKQDAAYRESVLPKNLKKRLAVEAGVTLYWRAFVG
                     DEGDVIGVDTYGESAPAPAVFKHFGLTVDHVVARLEALM"
     gene            3527..>4194
                     /locus_tag="VHE37_06080"
     CDS             3527..>4194
                     /locus_tag="VHE37_06080"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HVT35128.1"
                     /translation="MAVKVGINGFGRIGRNVLRAAVQNFGDIEIVGINDLLEPDYLAY
                     MLQYDSVHGRFKGQLAVEGGMLVVNGKKIRLTQERDPANLKWKDVGADVVIESTGLFL
                     DKASAQKHLDAGAKKVIISAPSKDDTPMFVFGVNHQTYAGQSIISNASCTTNCLAPLA
                     KVLHDKWGIKRGLMTTVHATTATQKTVDGPSSKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPSL"
BASE COUNT          806 a         1357 c         1345 g          686 t
ORIGIN      
        1 aactgggccc cctccatccg tctcgaggac ggcatggaga agacctacaa gtggatctac
       61 gacgagatga cctcgggcaa ggtctcggta gtcaacgcgc tcccgaaaca ggcgctcgcc
      121 gctcagtagg aaacaagcaa cgagaaagaa gaaggggccc gcgagggccc cttctccttt
      181 tcagcggccg atgccgcgga tgcccttgtt gctcttcagc agggtgtcga agtactcgat
      241 cgtcctgccg aggccggtgt cgaggtcgac cttcggctcc cacttgagct ccttgcgcgc
      301 cagcgtgatg tcggggcagc gctgcagcgg atcgtcgacc ggcagcggct gggtgtgcac
      361 gatcttcgac ttgccgttca cgagcttgac gatccgcgcc gccatctcct tgaccgggat
      421 ctccaccggg ttgccgatat tgaccgggcc ggtgaagtcg tccggcgtgt gatccatcag
      481 gcgcagccag ccgtcgagct ggtcgtcgac gtagcagaag gcgcgggtct gcatgccgtc
      541 gccgtagagc gtgatgtcct cgcccttcag cgcctggacg ataaagttgg acaccacgcg
      601 gccgtcgttg ggatgcatgt gcggcccgta ggtgttgaag atgcgcgcca ccttgatgcg
      661 cagcttgtgc tggcgatgat agtcgaagaa cagcgtctcg gcgcagcgct tgccctcgtc
      721 gtagcacgag cgcgggccga tgggattgac gttgccccag tattcctccg gctgcggatg
      781 gacggcggga tcgccgtaga cctcgctggt ggaggcctgg aagatcttgg cccggcaccg
      841 cttggccagg cccagcatgt tgatcgcgcc gtggacggac gtcttggtcg tctgcaccgg
      901 atcgaactgg tagtgaatcg gcgaggcggg gcaggcgagg ttgtagatct cctccacctc
      961 gacatagagc gggaacgtca cgtcgtggcg catgagctcg aagctcgggt ggtcgagcag
     1021 ctccagcacg ttgcggcgcg agcccgtata gaagttgtcg acgcacagga cttcgtgtcc
     1081 cgccgccacg agccgctcgc agaggtgcga gccgatgaag ccggcgccgc cggtgacgag
     1141 aacgcgcttg gggccgtatt cgtattcgct gcgcatgccg ttcctggaaa attgatgatg
     1201 cgccgattct aaatcagccc caccgcactc cggtttggca tgttataaaa aatttaatat
     1261 ttataaaata aaacaataat taataacatc aaaaatagcg gtgcggtgga gacattccag
     1321 gcttatcccg tgccggtatt gccgcagcgg atgcgaaccc cgacaatacg cgccctttcg
     1381 ctttccccct gcggaaatcc gccgcgactt tcaacaaaag cagcaacgcc gagatgccca
     1441 atcgcctgga actcgccaac gccatccgcg cgctggccat ggacgcggtg cagaaagcca
     1501 actccggaca tccgggcatg ccgatgggca tggccgacat cgccgaggtg ctgtggaacg
     1561 atttcctcaa gcacaacccg gccgatccga aatggcccga ccgcgaccgc ttcgtgctca
     1621 gcaacggcca cggctcgatg ctgctgtacg cgctgctgca cctgaccggc tacgacctgc
     1681 cgatgtccga actgcagaat ttccgccagc tgcacagcaa gaccgccggt cacccggaat
     1741 acggcatcac gccgggtgtg gaaaccacca ccggcccgct gggccagggc ctggccaacg
     1801 cggtcggctt cgcgctggct gagcgcctgc tcgccgcgcg ctacaaccgc gacggtcacg
     1861 acatcgtcga tcactacacc tatgtcttca ccggcgacgg ctgcctgatg gaaggcatca
     1921 gccacgaggc ctgctcgctg gccggcacgc tggggctgaa caagctcatc gtgctgtacg
     1981 acgataacaa catctccatc gacggcgaag tcgaaggctg gttccgcgac gacacgccgg
     2041 cgcgcttcga agcgtacggc tggcaggtga tccgcaacgt caacggccat gacgcgcgcg
     2101 acgtgaagaa ggccatcgag accgcacgcg ccagcgccga caagcccacg ctgatctgct
     2161 gcaagaccat catcggctac ggcgcaccga cgctgcaggg caaggagaaa tgccacggcg
     2221 caccgctggg tgacgccgag atcacgctgg cccgcacgca gctcaagtgg ccgcacgcgc
     2281 cgttcaccgt accggccgat gtcgccgccg gctggaacgc gcgcgagcgc ggcgccagcg
     2341 cgcagcgtgc gtggcaggaa aaattccaag cctatgcggc taagttcccc gccgaggcgg
     2401 cggaattctc gcgccgcatg aaaggcgaac tgccgagtga atggaagacc gcgatcgacg
     2461 cggtgatcgc gcagctgcag aaggccgaca agcccgacgc ctcgcgcaag tcgagcaagg
     2521 ccgtgctcaa catcatcgcc ccggccttgc ccgaactgct cggcggctcg gccgacctgt
     2581 ccgaatccaa ctgcaccgat ttcaagggct acctgccgct gcgccacggc aagcttgccg
     2641 gcaactacat caactacggc gtgcgcgaat tcggcatgag cgcgacgctc aacgggctgg
     2701 cgctgcacgg cggcttcatc ccgttcggcg gcaccttcct gaccttctcc gactacgcgc
     2761 gcaatgccat ccgcatggcg gcgctgatga agatcggcag catcttcgtc tacacgcacg
     2821 attccatcgg cctgggcgaa gacggcccga cgcaccagtc ggtggagcac gcggcctcgc
     2881 tgcgcctgat tccgaacgtg gagttctggc gccccggcga tgcgttcgaa accgcgatcg
     2941 cctggaaggc ggcggtcgag cgccgcaacg gcccgagcct gctcgcgctg tcgcgccaga
     3001 acctcaacgc gcaggcgcac cccgatgcgc acacggctgc ggccgcgcgc ggcggctacg
     3061 tgctgtggga gccggccacc gcgccgcagg cggtgattct tgctaccgga tcggaagtgc
     3121 agctggcggt ggacgcggca aagaagctgg ccggcaaact cgcggtgcgc gtggtctcgc
     3181 tgccctgcgt cgagcgcttc atgaaacagg acgcggctta ccgcgagtcg gtgctgccga
     3241 agaacctgaa gaaacgcctc gcggtcgaag cgggggtgac gctgtactgg cgcgccttcg
     3301 tcggcgacga gggtgacgtg ataggcgtgg acacctacgg cgaatcggca ccggcgccgg
     3361 cggtgttcaa gcatttcggt ttgaccgtcg accacgtggt cgcgcggctg gaagctttga
     3421 tgtaggagcg cgccttgcgc gcgatttgtc gcttgctcgc gcgcaaggtg cgctcctaca
     3481 agagcaagtc gtcgttcgag agtgggttta aacagaggac agagcaatgg cagtgaaagt
     3541 aggcatcaac ggattcggac gcatcggccg caacgtgctg cgcgcggcgg tgcagaactt
     3601 cggcgacatc gagatcgtcg gcatcaacga cctgctcgag ccggactacc tcgcctacat
     3661 gctgcagtac gactcggtgc acggccgctt caagggccag ctcgcggtgg aaggcggcat
     3721 gctggtggtc aacggcaaga agatccgcct gacccaggag cgcgatccgg cgaacctgaa
     3781 gtggaaggac gtgggcgccg acgtggtgat cgaatccacc ggcctgttcc tggacaaggc
     3841 ttccgcgcag aagcacctcg atgccggcgc gaagaaggtg atcatctcgg cgccgtcgaa
     3901 ggacgacacg ccgatgttcg tcttcggcgt caaccaccag acctacgccg gccagagcat
     3961 catttccaac gcctcctgca ccaccaactg cctcgcgccg ctggccaagg tgctgcacga
     4021 caaatggggc atcaagcgcg gcctgatgac cacggtgcac gccaccaccg cgacgcagaa
     4081 aaccgttgac ggcccgagca gcaaggactg gcgcggcggc cgcggcattc tcgaaaacat
     4141 catcccgtcc tcgaccggcg ccgcgaaggc cgtgggcgtg gtgattccct cgct
//
LOCUS       DASZZC010000290         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_665829,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000290 DASZZC010000000
VERSION     DASZZC010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665829"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VHE37_06085"
     CDS             complement(<1..316)
                     /locus_tag="VHE37_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35129.1"
                     /translation="MKKSFVLLFGMAAALPLYAQTDGSAPADAAPAAADSSSSSGSDA
                     APAAPAASSDNSAAPAADAAAPAADSSAAPAAGDAAAAPADSGAAASSDAAPAAADAA
                     AAP"
     gene            460..1398
                     /locus_tag="VHE37_06090"
     CDS             460..1398
                     /locus_tag="VHE37_06090"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVT35130.1"
                     /translation="MLAAGLAACAAAPETPHPALLPTSAAPEVIASVQVHGPEAGEDL
                     DAALDSLRGQKLDAALADTVSAYVRDYYGWLEWSHVQVIVDSALDAQGTLHVIASGDP
                     PADEPAAPLAMAAPAADAPAAPAGPSGARAESALDASFGPWLGSSSRVLIDAAEKRLY
                     LKRGGGDVVSYGVAVGTARTPTPPGDYTIEAIRSQPTWYPPASIRREYQRKGQSLPAA
                     VPPGAHNPLGSWFVRLQNGIGIHGTNQPRSIGHAVSHGCIRMHDRDVAELIRALKRGD
                     GVTIVRSRAAQWALKEDSDEVLRRLRPAGGSQTASR"
     gene            1343..1918
                     /locus_tag="VHE37_06095"
     CDS             1343..1918
                     /locus_tag="VHE37_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVT35131.1"
                     /translation="MKYCADCGQPVEVRQPPGDHLPRHMCPACGAIHYRNPKLIVGVV
                     PEWQDGRVLMCRRAIEPRLGLWTFPAGFMELGETSAQGAAREAFEEAGAEVESGSLLC
                     VINVPYVNQVYLIHRGKLLAEKFHPTPESSEVCLMREDEIPWDEIAFPTIYRGLKYFF
                     EDRASGSQGVHTLDLMQRPRPPQLAEVTSGA"
     gene            1968..2291
                     /gene="fdxA"
                     /locus_tag="VHE37_06100"
     CDS             1968..2291
                     /gene="fdxA"
                     /locus_tag="VHE37_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HVT35132.1"
                     /translation="MTFVVIENCIKCKYTDCVEVCPVDCFHEGPNMLVIDPEECIDCT
                     LCEPECPAEAIVAEDDLPADQAHFKELNAELSKNWPVITEQKAAPEGAKDWDGKPDKL
                     KLLER"
     gene            complement(2311..3618)
                     /locus_tag="VHE37_06105"
     CDS             complement(2311..3618)
                     /locus_tag="VHE37_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT35133.1"
                     /translation="MSRILRRIFPLWLALLAAPALAQGPTQQIDAALLQAKARHAPVL
                     VDFHAPWCYSCYYMKQNVLTGHEWERVERETVVVSLDADAPEGAHWMDAWHVKALPSY
                     VVLDEKGEELGRIIAEQTRYNFYRQLDIITARGSTLAALQAKAAKGGLPGLAAARDAL
                     KAFLARRDASTGLDWFAALPPAVRAPMAEDPKVRQTLAQLRLQKAALAKDNTACISAG
                     EEVLKSGLGCEFPYELDTVMACAADLPQPQREKLLAGHRHAMDHLVTFGAFGSKRCAD
                     QRSIVFTAADLYESLDDKTAAAKLLDRAIADAEKKLGGDPRKDRNLADNLRVYLDRAG
                     RTAQLDAWMLKLIAAYPGDYVYNYRHGKNLLARGQAAEALPFLERAADKAYGINRLKV
                     AQQRAEALKALKRDDDARQVAADALKANGPWFPEEAEKLKVYL"
     gene            complement(3636..>3926)
                     /locus_tag="VHE37_06110"
     CDS             complement(3636..>3926)
                     /locus_tag="VHE37_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35134.1"
                     /translation="FIAAGRQDTFKFRARRVEDQVIDGKTGVQFLIEPDSLLRWLVDP
                     LHVVYDPAARMLLEFRGVSNVINPATGEEYNVRIEYRAQPPPDAPPRLPPLG"
BASE COUNT          604 a         1343 c         1344 g          635 t
ORIGIN      
        1 ccggggccgc ggcggcatcg gcggcagccg gcgcggcgtc ggaggacgct gcggcgccac
       61 tgtcggccgg tgccgctgcc gcatcgcctg cggccggtgc tgcactgctg tcggccgcag
      121 gggcggctgc atcggcagcc ggggcggcgc tgttgtcgga agatgctgca ggcgcagcag
      181 gagccgcgtc gctgccgctg gaggaactgc tgtcggcggc agcgggcgcc gcgtcggccg
      241 gggcgctgcc gtcggtctgt gcgtacagcg gcagagctgc cgccatgccg aacaagagga
      301 cgaaagattt tttcattatg gtctccccgc agagccgggc cggatttttt gatggcggtg
      361 atattcaccg atttctccgc gcaaatctag cagcgtgctt gctaatcttc gcgcatggga
      421 gcgtcctggt ttttgcgctg tttgcgctgc gcgccggctg tgctggcggc gggcctcgcc
      481 gcctgcgcgg cggcgccgga gacaccacac ccggcactgt tgccgacctc tgccgcaccc
      541 gaggtgatcg cctcggtgca ggtccatgga ccggaagccg gggaagatct cgatgcggcg
      601 ctcgacagcc tgcgcggaca gaaacttgac gcggcgctcg ccgacaccgt ctcggcttac
      661 gtgcgcgatt actacggttg gctcgagtgg agccatgtgc aggtgatcgt cgacagcgcg
      721 ctcgatgcac aaggcacgct gcacgtgatc gccagcggcg atccgccggc cgacgaaccg
      781 gccgccccgc tggcgatggc cgcacccgcc gcagatgctc ccgctgcgcc cgccggcccg
      841 agcggcgcgc gcgccgagtc cgcgctcgat gcgagcttcg ggccgtggct cggttcatcc
      901 agccgcgtgc tgatcgacgc ggcggaaaag cgcctgtacc tcaagcgcgg cggcggcgac
      961 gtggtgagct acggcgtcgc cgtcggcacc gcccgcacac cgacgccgcc gggcgactac
     1021 accatcgagg cgatccgctc gcagcccacc tggtatccgc cggccagcat ccgccgtgaa
     1081 taccagcgca agggccagtc gctgccggcg gcggtaccgc cgggagcgca taatccgctg
     1141 ggcagctggt tcgtgcggct gcagaacggc atcggcatcc acggcaccaa ccagccgcgc
     1201 tcgatcggcc acgcggtgtc gcatggctgc atccgcatgc acgaccgcga cgtggccgaa
     1261 ctgatccggg cgctcaagcg cggcgatggc gtcaccatcg tccgctcgcg cgccgcgcaa
     1321 tgggcattga aggaagacag cgatgaagta ctgcgccgat tgcggccagc cggtggaagt
     1381 cagacagcct cccggtgatc acctgccgcg gcacatgtgc ccggcctgcg gcgccatcca
     1441 ttaccgcaac cccaagctga tcgtcggcgt ggtgccggag tggcaggatg gacgcgtgct
     1501 gatgtgccgc cgcgccatcg aaccgcgcct gggactgtgg acctttcccg ccggcttcat
     1561 ggagctgggc gaaaccagcg cgcagggcgc ggcacgcgag gcgttcgagg aggccggcgc
     1621 cgaggtcgag tccggctcgc tgctgtgcgt gatcaacgtg ccttacgtca accaggtcta
     1681 cctgatccac cgcgggaagc tgctggcgga gaaattccat cccaccccgg aaagctccga
     1741 ggtgtgcctg atgcgcgaag atgaaatccc ctgggacgag atcgccttcc ccaccatcta
     1801 ccgcggactc aagtatttct tcgaagatcg cgccagcggc agccagggcg tgcacacgct
     1861 ggacctgatg cagcggccgc ggccaccgca gcttgcggaa gtgaccagcg gcgcgtagct
     1921 gctgctagaa ttcgcgccgc gatttatgtc cgtagcgtga gaggcccatg actttcgtcg
     1981 tcatcgaaaa ctgcatcaag tgcaagtaca ccgactgtgt cgaggtctgc ccggtcgact
     2041 gcttccacga agggccgaac atgctggtga tcgatcccga ggaatgcatc gactgcaccc
     2101 tgtgcgagcc ggaatgcccg gccgaggcga tcgtggcgga agacgacctg cccgccgacc
     2161 aggcgcattt caaggaactc aacgccgagt tgtcgaagaa ctggccggtc attaccgagc
     2221 agaaagccgc tcccgagggc gccaaggact gggacggcaa gccggacaag ctcaagctgc
     2281 tcgagcgctg agccggcgcg cggtccgcgc ctagaggtac accttcagct tttccgcttc
     2341 ctcgggaaac cacggcccgt tggccttgag cgcgtccgcc gccacctgcc gcgcgtcgtc
     2401 gtcgcgcttg agcgccttca acgcctccgc gcgctgctgc gccactttca gccggttgat
     2461 gccataagcc ttgtctgccg cgcgctcgag aaacggcagc gcctccgccg cctgtccgcg
     2521 cgccagcagg ttcttgccgt ggcggtagtt gtagacgtaa tcgccagggt aagccgcgat
     2581 cagcttgagc atccaggcgt cgagctgcgc ggtgcggccg gcgcggtcga gatagacgcg
     2641 caggttgtcg gccaggttgc ggtccttcct cggatcgccg ccgagcttct tctcggcatc
     2701 ggcgatggcg cggtccagca gcttggccgc ggcggtcttg tcgtcgagcg actcgtacag
     2761 gtccgccgcg gtgaacacga tgctgcgctg gtcggcgcag cgcttgctgc cgaaggcgcc
     2821 gaaggtgacc aggtggtcca tcgcgtgccg gtgcccggcc agcagcttct cgcgctgggg
     2881 ctgcggcaga tcggcggcgc aggccatgac ggtatccagc tcgtagggaa actcgcagcc
     2941 cagaccgctc ttcagcactt cctcgccggc gctgatgcag gcggtgttgt ccttggccag
     3001 cgcagccttc tgcaggcgca gctgcgccag cgtctgccgc accttcgggt cttcggccat
     3061 cggtgcacgc accgccggcg gcagcgcggc gaaccagtca aggccggtcg acgcgtcgcg
     3121 gcgcgcgagg aaggccttga gcgcatcgcg cgccgcggcc agcccgggca gcccgccctt
     3181 ggccgccttg gcctgcagcg cagccagcgt gctgccgcgc gcagtgatga tgtcgagctg
     3241 cctgtagaag ttgtagcgcg tctgctcggc gatgatgcgg ccgagttcct cgcccttttc
     3301 atccagcacc acgtagctgg gcagcgcctt gacgtgccag gcatccatcc agtgcgcgcc
     3361 ctcgggcgcg tcggcatcga gcgacaccac caccgtctcg cgctcgacgc gctcccattc
     3421 gtgcccggtc agcacgttct gcttcatgta gtagcaggaa tagcaccagg gcgcgtggaa
     3481 atcgaccagc accggcgcgt ggcgtgcctt ggcctgcagc agtgcggcgt cgatctgctg
     3541 cgtggggccc tgcgccagcg ccggagcggc cagcagcgcc agccatagcg ggaagattcg
     3601 cctgaggatt cggctcatgc cgcgattcta gccgctcacc ccagcggcgg cagcctgggc
     3661 ggcgcgtccg gcggcggctg cgcccggtac tcgatgcgca cgttgtactc ctcgccggtt
     3721 gccgggttga tcacgttgga tacgccgcgg aattccagca gcatgcgcgc ggccgggtcg
     3781 tacaccacgt gcagcggatc gaccagccag cgcagcaggg aatcgggttc gatcaggaac
     3841 tgcaccccgg tcttgccgtc aatcacctgg tcctcgacgc ggcgggcgcg gaacttgaac
     3901 gtatcctggc gcccggcggc aatgaa
//
LOCUS       DASZZC010000291         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1048257,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000291 DASZZC010000000
VERSION     DASZZC010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048257"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VHE37_06115"
     CDS             <1..672
                     /locus_tag="VHE37_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35135.1"
                     /translation="ADPLKPRVASIIDLPDARAAQQQFRYLFVTTARGLEVIDMTDPA
                     HPRALPDAHVPLADAQRVYLARTYAYVAAGAQGLAIVDIEKAEAPKLLQLYNADGKLT
                     DARDVIVGSTNASLFAYVADGRNGLQVLQLTAPDTQPKFYGFSPEPKPQLIAHYATPA
                     PALALSRGLDRDRAVDESGGQIAVFGRIGSRPFTLPEMQKLYLDAGSAPWFVSNQDEH
                     APRAR"
     gene            740..1210
                     /locus_tag="VHE37_06120"
     CDS             740..1210
                     /locus_tag="VHE37_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35136.1"
                     /translation="MKRLLCGAALLALASSAGAAGFGAAAPATGPNGTSNSTAPPAGI
                     SAPTAPSSAAAGGPVANPYSATPGAPYAPGQTPATLPGAPIPLPNPPPLPGNAASTTG
                     AGVSGGAMEFNQLDTSGDGSLSQQESGMTQEQFNARDANHDGRLSPLEFSNPLP"
     gene            complement(1322..3373)
                     /gene="ligA"
                     /locus_tag="VHE37_06125"
     CDS             complement(1322..3373)
                     /gene="ligA"
                     /locus_tag="VHE37_06125"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HVT35137.1"
                     /translation="MSVPKAAKERAEVLRRELHEHNHRYYVLDQPSIGDAEYDRLFAE
                     LQQLEQQHPELLTPDSPTQRVGGAPRADFEPVKHRLPMQSLRKCNDEQELRDFDRRIC
                     ELLGRTTVDYVAEPKLDGLAVSLTYENGVLARGATRGDGETGEDITENLKTIRRVPLK
                     LRGKAPPVVEVRGEVYLPLAGFRRWVEDAMAKGEKPPVNPRNAAAGSLRQLDPKITAT
                     RPLAFYAYAVGYSEGWKSPALHSQVLENLRAWGFPVSERIEVVHSVDECIRYFEKTGA
                     QRSKLPFDIDGVVFKLDDLAGREEVGSVSREPRWACAYKFAAEEAETRLRDVEFQVGR
                     TGALTPTARLEPVFVGGATVSNATLHNMDEVARKDVRIGDTVVVRRAGDVIPEVKEVV
                     LARRPPDARVVQPPKVCPECGSPAERAEGEVVWRCTGGLSCASQAQGMLLHFAGRRAL
                     DIEGLGEKLLLQLIEKKLVQLPVDIYRLDLATLSKLDRMGEKSAQNVLDAIERSKATT
                     LARFIYALGIPDVGESTAATLSAHFRTLEALMQAAEEDLPTQQEEKEKNRFPKLREVP
                     DVGPEVARRIAQWFALPTHRQLVADLRAAGIHWPDAPAAPSSGPLLGKTFVITGTHAV
                     SRDAVTEFIEQHGGKVSGSVSAKTDYLVAGEAAGSKLAKAEKLGVAIVDWDGLQKLAN
                     G"
     gene            complement(3384..>3795)
                     /locus_tag="VHE37_06130"
     CDS             complement(3384..>3795)
                     /locus_tag="VHE37_06130"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HVT35138.1"
                     /translation="EKIFALLIAEREGTAIMGPKLHAALLQQSLQFGARKIYHRMSKG
                     MVVFSVASLLKPGELDPAQAQGFSTPGLSVFMVLPGPIKPAAAYADMVATARALARSL
                     NAEVFDSRKQLFGAEAERAQKVEIEEWARANRLA"
BASE COUNT          586 a         1348 c         1220 g          641 t
ORIGIN      
        1 gccgatccgc tcaagccgcg cgtcgcttcg atcatcgacc tgcccgatgc gcgcgcggcg
       61 cagcagcagt tccgctatct cttcgtcacc accgcgcgcg ggctggaggt gatcgacatg
      121 accgatcccg cgcacccgcg cgcgctgccc gacgcgcatg tgccgctggc ggacgcgcag
      181 cgcgtgtatc tcgcgcgcac ctacgcctac gtggccgcgg gcgcgcaggg cctggccatt
      241 gtcgacatcg agaaagcgga agcgccgaag ctgctgcagc tctacaacgc cgatggcaag
      301 ctcaccgacg cgcgcgacgt gatcgtcggc agcaccaatg cttcgctgtt cgcctacgtg
      361 gccgacggcc gcaatggact gcaggtgctg cagctcaccg cgccggacac gcagccgaag
      421 ttctatggtt tttcccccga gcctaaacca cagctgatcg cgcactacgc cacgcccgcg
      481 cccgcgctgg cgctgtcgcg cgggctcgac cgcgatcgcg cagtggacga gagcggcggg
      541 cagatcgcgg tgttcggccg catcggatcg cggccgttca cgctgccgga gatgcagaag
      601 ctctacctcg acgccggcag cgcgccatgg ttcgtcagca accaggatga gcacgcgcca
      661 cgcgcgcgtt gagcggaaat cacctgcaac cttcggcggg ctcccgggtc ggttttatat
      721 agcccttcgg agcagaccga tgaaacgatt actttgcggc gccgcgctgc tcgcgctcgc
      781 cagcagcgca ggagcggcgg gcttcggcgc ggctgcgccc gcaaccggac ccaatggcac
      841 gtcgaacagc acggctccgc cggccgggat cagcgcgccg acggcgccga gctccgccgc
      901 ggccggcggc cccgtcgcca atccctactc ggccacgccg ggcgcaccgt acgcgcctgg
      961 acagacgccc gccacgctgc ccggcgcgcc gattccgctg cccaatccac cgccactgcc
     1021 cggcaatgcc gcgagtacca cgggcgccgg tgtcagcggc ggcgccatgg agttcaacca
     1081 gctcgacacc agcggcgacg gcagcctgag ccagcaggaa tcgggcatga cccaggagca
     1141 gttcaacgcg cgcgacgcca accatgacgg ccggctgagc ccgctggagt tttccaatcc
     1201 gctgccgtga ggtttttttt gccggaccgc accttcgatt tcgctgttat ttattatctt
     1261 atgatttatt ttatatataa taattaataa cagactgaaa cgccgtgcgg ttaggccctt
     1321 ttcagccgtt tgcgagcttc tgcaagccgt cccagtcgac gatggccacg ccgagcttct
     1381 ccgccttggc cagcttggag ccagccgctt caccggcaac gagatagtcg gtcttggccg
     1441 acaccgagcc gctgaccttg ccgccgtgct gctcgatgaa ttcggtcacc gcgtcgcggc
     1501 tcaccgcgtg cgtgccagtg atgacgaaag tcttgcccag cagcgggccg ctgctcggcg
     1561 cagccggcgc gtcgggccag tggatgccgg ccgcgcgcag gtcggcgacc agttgccggt
     1621 gcgtgggcag cgcgaaccac tgcgcgatgc gccgcgcaac ttccggcccc acgtcgggca
     1681 cttcgcgcaa tttcggaaag cggttcttct ctttttcttc ctgctgcgtc ggcaaatcct
     1741 cttccgccgc ctgcatcaag gcttccagcg tgcggaaatg cgcagacagc gtggccgctg
     1801 tggattcgcc aacatcggga atgcccagcg cataaatgaa gcgcgccagc gtggtggcct
     1861 tgctgcgctc gatggcatcg agcacgttct gcgcggactt ctcgcccatg cgatcgagct
     1921 tggacaaggt ggcgagatcg agccggtaga tgtcgaccgg cagctgcacc agcttcttct
     1981 cgatcagctg cagcagcagt ttctcgccga ggccctcgat gtcgagagcg cgccggccgg
     2041 cgaaatgcag cagcatgccc tgcgcctgcg aggcgcaact caacccgccg gtgcagcgcc
     2101 acaccacttc gccttcggcg cgctcggccg ggctgccgca ctccgggcaa accttcggcg
     2161 gctgcaccac gcgcgcgtcg ggcgggcgcc tggcgagcac cacttccttc acttccggga
     2221 tcacgtcgcc ggcgcggcgc acgacgacgg tgtcgccgat gcgcacgtcc ttgcgcgcga
     2281 cctcgtccat gttgtgcagc gtggcgttgc tcacggtggc gccgccgacg aacaccggct
     2341 ccagccgcgc ggtgggcgtg agcgcgccgg tgcggccgac ctggaactcg acgtcgcgca
     2401 gccgcgtctc ggcttcttcc gccgcgaact tgtaggcgca ggcccagcgc ggctcgcgcg
     2461 acaccgagcc cacctcctcg cgcccggcca ggtcatcgag cttgaacacc acgccgtcga
     2521 tgtcgaacgg cagcttgctg cgctgcgcgc cggttttctc aaagtagcgg atgcactcat
     2581 cgaccgaatg caccacttcg atccgttcgg ataccgggaa gccccaggcg cgcagattct
     2641 ccagcacctg cgagtgcagc gcgggtgatt tccatccctc gctgtagccg accgcgtagg
     2701 cgtagaacgc cagcggccgc gtggcggtga ttttcgggtc gagctggcgc aggctgccgg
     2761 ctgcggcatt gcgcggattc accggcggct tctcgccctt ggccattgcg tcttccaccc
     2821 agcggcggaa cccggccagc ggcaggtaga cctcgccgcg cacctcgacc accggcggcg
     2881 ccttgccccg cagcttcagc ggcacccgcc ggatggtttt caggttctcg gtgatgtcct
     2941 cgccggtctc gccgtcgcca cgcgtggcgc cgcgcgccag cacgccgttc tcgtaggtga
     3001 gcgacacggc gagcccgtcg agcttgggct cagccacgta atcgactgtg gtgcgcccga
     3061 gcagttcgca gatgcggcga tcgaagtcgc gcagctcctg ctcgtcgttg cacttgcgca
     3121 gcgactgcat cggcagcctg tgcttgaccg gttcgaaatc ggcacgcggc gcgccgccga
     3181 cgcgctgcgt cggcgaatcc ggggtgagca gttcgggatg ctgctgttcg agctgctgca
     3241 gctcggcgaa caggcggtcg tactcggcgt cgccgatgct cggctggtcg agcacgtaat
     3301 agcggtggtt gtgctcgtgc aactcgcggc gcaggacctc ggcgcgttcc ttcgctgcct
     3361 tgggcacgct catgcgaaaa atctcaggcg aggcgattgg cccgcgccca ctcctcgatc
     3421 tcgaccttct gcgcgcgctc ggcctcggcg ccgaacagct gcttgcgcga gtcgaacact
     3481 tcggcgttca gcgagcgcgc cagcgcgcgc gcagtggcca ccatgtcggc gtaggccgcg
     3541 gccggcttga tcggccccgg cagcaccatg aacaccgaca gtcccggcgt ggaaaaaccc
     3601 tgcgcctgcg ccggatcgag ttcgcccggc ttgagcagcg acgccaccga gaacaccacc
     3661 atgcccttgc tcatgcggtg gtagatcttg cgcgcgccaa actgcaggct ctgctgcagc
     3721 agcgcggcgt gcagcttggg acccatgatg gcggtacctt cgcgctcggc gatcagcagc
     3781 gcgaaaatct tttcc
//
LOCUS       DASZZC010000292         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_988003,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000292 DASZZC010000000
VERSION     DASZZC010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988003"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VHE37_06135"
     CDS             complement(<1..255)
                     /locus_tag="VHE37_06135"
                     /inference="COORDINATES: protein motif:HMM:NF023687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3613 domain-containing protein"
                     /protein_id="HVT35139.1"
                     /translation="MKKLIAGLAVLALGGCSDIPTRVGSQTQAWTDLQKSNMASSAAP
                     RPMPGEVADKVYQRYVNSFGNPIPDLYTRDTANSGGGSSGG"
     gene            complement(270..908)
                     /locus_tag="VHE37_06140"
     CDS             complement(270..908)
                     /locus_tag="VHE37_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT35140.1"
                     /translation="MLALLTALVLSACASSASQDSAHSEQEAGNELHDDLIRTMLTQK
                     KYYAALAHVQQQEAISGDTPQLRYLEGEARRNLGQDAVAEELYKSLLKTPYAAEGRHG
                     LGLLYAKVDLRAAVWQLQQAVQRRPTDAEMRNDLGYALMVAGRFNEAMPQLATAAELD
                     PDDTRSRNNLVILLLLTHDEAGVKRIAGESGMSADTLSGLRKQAAAFKVRKS"
     gene            complement(1023..1958)
                     /locus_tag="VHE37_06145"
     CDS             complement(1023..1958)
                     /locus_tag="VHE37_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT35141.1"
                     /translation="MSAGAFAVLAAFACLLMVAAVFAVALLVQRTRGESKLQRRFEPE
                     LAGAAEFEIAGQKPLITNIARGGKAIEGWVDPENESARLMVQAGWRSDTARAMWYVVQ
                     ALVPLAGFGSVVLYWALGPEQHKSLFLLLFAFVAFALSMLIPRWILRGAAGSRRDRIK
                     GEVPLFIHLLVLLFEAGLSTRQAIASLVREGAGVLPILGGEFELVLRQLEAGGDTSEV
                     LKNLTDMLMVDDLTSAIGVLRQVDRYGGEVREPLLEALAVLEQRRGLDLREKVNLLSG
                     RMTVVMVLFFFPALLIFVAGPAFLSIIRALHNVSH"
     gene            complement(1955..>2612)
                     /locus_tag="VHE37_06150"
     CDS             complement(1955..>2612)
                     /locus_tag="VHE37_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT35142.1"
                     /translation="VPVALLVFGVLGGIVVTGVIVLVAWFVLARRAAARRALLLEQLP
                     PFLDSAMRVLSAGNTLEESIAAAARESPEPLRPLMSSVGRQVRLGAPIEAVLMEAGDI
                     NRLRDIKVMALAAGINRKFGGSMKNILRSLIQTIRQRDVAARELRALTAETRFSAIVL
                     SIIPASLSIYIYLKNPKYYHDMWVDPVGRWLLVVSILMQFIGVFLIMRMMATTSEGDA
                     "
BASE COUNT          447 a          905 c          828 g          432 t
ORIGIN      
        1 cccgccactg ctgccaccgc cgctgttcgc cgtgtcacgc gtgtacagat ccgggatcgg
       61 attgccgaag ctgttgacgt aacgctggta gaccttgtcg gccacttcgc ccggcatcgg
      121 ccgcggcgcc gcgctggagg ccatgttgct cttctgcagg tcggtccagg cctgcgtctg
      181 cgagcccacc ctggtcggaa tgtcggaaca tccgccgagc gccagcaccg ccagacccgc
      241 gatcagtttc ttcatgtcca tctctccggt caggactttc tcaccttgaa cgccgccgcc
      301 tgcttgcgca agccggacaa cgtatcggcc gacatgccgc tttcgccggc aatgcgcttg
      361 acgccggcct catcgtgcgt gagcaacaac aggatcacca ggttgttgcg gctgcgcgtg
      421 tcgtccgggt cgagttccgc cgccgtcgcc agctgcggca tggcctcgtt gaaacgtccc
      481 gccaccatca gggcatagcc tagatcgttg cgcatttccg cgtcggtcgg ccgccgttga
      541 acggcctgct ggagttgcca gacggcagcc cgcagatcga ccttggcgta gagcaggccc
      601 aggccatggc ggccttccgc cgcgtagggc gtcttgagca ggcttttgta cagttcctcg
      661 gccactgcat cctgaccgag gttgcggcgc gcctcgcctt ccaggtaacg cagctgtggc
      721 gtgtcgccgg agatcgcttc ctgctgctgc acgtgggcca gcgcggcgta gtacttcttc
      781 tgcgtaagca tggtgcggat caggtcgtcg tgcagctcgt tgccggcttc ctgctcgctg
      841 tgggcgctgt cctgcgacgc gctgctggca cacgccgaca gcaccagcgc cgtcagcagg
      901 gccagcaagg ctccaccgca gcagcatttc acttgttcta aaatattgaa gttatttaca
      961 atcattttat tttttataac atcaagatcc gggatgcggt ggccggaaaa ttcccggaac
     1021 gatcagtgcg aaacgttgtg cagcgcgcgg atgatcgaca ggaacgccgg gccggcgacg
     1081 aagatcagca gcgccgggaa gaagaacagc accatcacca ccgtcatgcg gccggacagc
     1141 aggttgacct tctcgcgcag gtccaggccg cggcgctgct ccagcacggc gagcgcctcg
     1201 agcagcggct cgcgcacttc gccgccgtag cggtcgacct ggcgcaaaac tcctatcgcg
     1261 ctggtcaggt catccaccat cagcatgtcg gtcaggttct tcagcacttc ggacgtgtcg
     1321 ccgcccgctt ccagctggcg cagcaccagc tcgaattcgc cgccgaggat cggcagcacg
     1381 ccggcgccct cgcgcaccag gctggcgatg gcctggcgcg tggacaggcc cgcctcgaac
     1441 agcagcacca gcaggtggat gaacagcggc acctcgccct tgatgcggtc gcgccggctg
     1501 ccggcggcac cgcgcaggat ccagcgcggg atcagcatcg acagcgcgaa ggcgacgaag
     1561 gcgaacagca gcaggaacag cgatttgtgc tgctccggcc ccagcgccca gtacagcacc
     1621 accgatccga aaccagccag cggcaccagc gcctgcacca cgtaccacat ggcgcgtgcg
     1681 gtgtcgctgc gccagcccgc ctgcaccatc agccgcgcac tttcgttttc cgggtcgacc
     1741 cagccctcga tggccttgcc gccgcgcgcg atgttggtga tgagcggctt ctgcccggcg
     1801 atctcgaact cggccgcgcc ggccagttcc ggttcgaagc ggcgctgcag cttggattcg
     1861 ccgcgcgtgc gctgcaccag cagcgccacc gcaaacaccg ccgccaccat cagcaggcag
     1921 gcgaatgcgg cgaggaccgc gaaagcgccg gcgctcatgc gtcaccctcg ctggtggtgg
     1981 ccatcatgcg catgatcagg aacacgccga tgaactgcat gaggatggag accaccagca
     2041 gccagcggcc gaccggatcg acccacatgt cgtggtagta cttgggattc ttcaggtaga
     2101 tgtagatcga cagcgacgcc ggaatgatgg acagcacgat ggcggagaag cgcgtctctg
     2161 cggtcagcgc gcgcagttcg cgcgcggcca cgtcgcgctg gcggatggtc tggatcaggc
     2221 tgcgcaggat gttcttcatg ctgccgccga acttgcggtt gatgccggcg gccagcgcca
     2281 tcaccttgat gtcgcgcagg cggttgatgt cgccggcttc catcagcacc gcctcgatcg
     2341 gcgcccccag gcgcacctgg cggcccaccg acgacatcag cggccggagc ggctcgggac
     2401 tctcgcgcgc ggcagcggct atcgattcct ccagcgtgtt gccggccgac agcacgcgca
     2461 tggcgctgtc gagaaacggc ggcagctgct ccagcagcag cgcacggcgc gcggcggcgc
     2521 ggcgcgccag cacgaaccag gcgacgagca cgatgacgcc ggtcaccacg ataccgccca
     2581 gcacgccgaa caccagcagc gccaccggca cc
//
LOCUS       DASZZC010000293         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_537933,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000293 DASZZC010000000
VERSION     DASZZC010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537933"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHE37_06155"
     CDS             <1..273
                     /locus_tag="VHE37_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT35143.1"
                     /translation="TGAGCLRALQERLPACVLCDLMMPGMNGTEFVRAAQLAGHRAPV
                     LIYTATPDSPLAREALQLGALGVLAKPLSPEKLETELRHAAGPAAG"
     gene            329..1312
                     /gene="dusB"
                     /locus_tag="VHE37_06160"
     CDS             329..1312
                     /gene="dusB"
                     /locus_tag="VHE37_06160"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HVT35144.1"
                     /translation="MFQLGLYRIDPPVALAPMAGVTDRPFRRLCRQLGAGYVVSEMTT
                     SDASLWHTEKSRLRRDHAGEVEPIAVQIAGYDPQQLADAARWNVEHGAQVIDINMGCP
                     AKKVCRVDAGSALLRDEALVARICAAVVNAVPVPVTLKIRTGYTRAQRNGVNIARIAQ
                     DCGIAMLAVHGRTREDHYEGAAEYDTIAAIKSQVRIPVLANGDIATPQAARAVFDHTR
                     CDGVMLGRAAQGRPWIFREIAHYLAHGELLPPPTLFERRRWLLEHLDELHDFYGGGRG
                     VRVARKHIQWYCRGNDGGDEFWRQVNQLTDARAQRDCVASFFERAPLSQAA"
     gene            complement(1442..2161)
                     /locus_tag="VHE37_06165"
     CDS             complement(1442..2161)
                     /locus_tag="VHE37_06165"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HVT35145.1"
                     /translation="MGQLHSRGSAAQEITSHAAIAQLLGRLLDNRCQLAVRLPGAPIT
                     YSTILLEVDFAGGSLLLDEICPAAGNAYVQVGGIARLQGRLDGSRVDFDCEVNAVANQ
                     NGTPSYRAGVPQRVIYYERRGSYRLTIPPELKLPPTMLSAQAGAMAGRLVDISREGAG
                     TLLSEADAELGVELACTIPLPDGQLSAEVEVRSARAGEDGLRVGLEFSHLTPAQRVSV
                     DRAIAALERTLLRHHVNARWM"
     gene            complement(2170..>2523)
                     /gene="flgN"
                     /locus_tag="VHE37_06170"
     CDS             complement(2170..>2523)
                     /gene="flgN"
                     /locus_tag="VHE37_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HVT35146.1"
                     /translation="SNLDELEQACADKLAAAQKLQALGLQLGKLQAAPAQIETLLAQA
                     PDAGASVARWRELLRLAAQCQQLNQDNGALLEQRQLQLRRTRSTLLGHRAAPATYGRS
                     GDSGFDLGRRSLASA"
BASE COUNT          379 a          842 c          903 g          399 t
ORIGIN      
        1 accggcgccg gttgcctgcg ggcgctgcag gaacgcctgc cggcctgcgt gctgtgcgac
       61 ctgatgatgc cgggtatgaa cggcaccgag ttcgtccgcg ccgcgcagct ggcgggccat
      121 cgcgcgccgg tgctcatcta caccgccacg ccggattcgc cgctggcgcg cgaggcactg
      181 cagctcggcg ccctgggcgt gctggccaaa ccgctgtcgc cggaaaagct cgagaccgaa
      241 ttgcggcacg ccgcgggccc tgccgccggc tgaaggcgct gcggcaggct gcggcataat
      301 ccgcgccgca gaatttccgg tgtcgttcat gttccagctg ggtctctacc gcatcgatcc
      361 gcccgtggcg cttgcgccca tggccggcgt caccgaccgc ccgttccgcc ggctgtgccg
      421 gcagctgggc gcgggctacg tggtgtcgga aatgaccacg tccgacgcca gcttgtggca
      481 cacggaaaaa tcgcgcctgc gccgcgacca tgccggcgag gtggagccga tcgccgtgca
      541 gatcgccggc tacgacccgc agcagctggc cgacgccgcg cgctggaacg tggagcacgg
      601 cgcgcaggtc atcgacatca acatgggttg cccggccaag aaggtgtgcc gcgtcgacgc
      661 cggcagcgcg ctgctgcgcg acgaggcgct ggtcgcgcgc atctgcgcgg cggtggtgaa
      721 tgcggtgccg gtgccggtca cgctgaagat ccgcaccggc tacacgcgcg cgcagcgtaa
      781 cggcgtgaac atcgcgcgca tcgcgcagga ctgtggcatc gccatgctcg cggtgcatgg
      841 gcgcacgcgc gaggatcatt acgagggtgc ggcggagtac gacaccattg cggcgatcaa
      901 atcgcaggtg cgcatcccgg tgctggccaa cggcgacatt gccacgccgc aggcggcgcg
      961 cgcggtgttc gaccacacgc gctgcgacgg cgtcatgctc ggccgcgcgg cgcagggccg
     1021 gccgtggatc ttccgcgaga ttgcgcatta cctggcgcat ggcgagctgc tgccgccgcc
     1081 cacgctgttc gagcggcgcc gctggctgct cgaacacctg gacgagctgc acgattttta
     1141 cggcgggggc cgcggcgtgc gcgtggcgcg caagcatatc cagtggtact gccgcggcaa
     1201 cgacggcggc gacgagttct ggcgccaggt caaccagctg accgatgcac gggcgcagcg
     1261 cgactgcgtg gcgtcatttt tcgagcgcgc gccgttgtcg caggccgctt gagagggtaa
     1321 ccgcactgcc cttgttgatg ttataaatta ttatgtaaaa taaaaataaa acaattattt
     1381 ataacaagag aaatgcgggt gcggtgaagg aaaaatcgcc ggctgcggcg atgtctgcgc
     1441 gctacatcca gcgcgcgttg acgtggtgcc gcagcagcgt gcgttccagc gcggcgatgg
     1501 cgcggtcgac cgacacgcgc tgcgccgggg tgagatggga aaattccagg ccgacgcgca
     1561 ggccgtcttc gccggcgcgg gccgagcgca cttccacttc cgccgacagc tggccgtcgg
     1621 gcagcgggat ggtgcaggcg agttccacgc ccagttccgc atcggcttcg ctcagcagcg
     1681 tgcccgcacc ttcgcgcgag atgtcgacca gccgcccggc catggcgccg gcctgcgccg
     1741 acagcatggt cggcggcagc ttgagttcgg gcggaatggt caggcggtag gagccgcggc
     1801 gctcgtaata gatgacgcgc tgaggcacgc cggcgcggta cgacggcgtg ccgttctggt
     1861 ttgccaccgc gttcacctcg cagtcgaaat ccacgcggct gccgtcgagc cggccctgca
     1921 ggcgcgcgat gccgcccacc tgcacgtagg cgttgccggc ggccgggcag atctcgtcca
     1981 gcagcagcga gccgccggcg aaatccactt ccagcaggat ggtcgagtag gtgatcggcg
     2041 cgccgggcag gcgcaccgcc agctggcagc ggttgtccag cagccgtccg agcagttgcg
     2101 cgatggcggc gtggctggtg atttcctgtg cggcgctgcc gcggctatgc agttggccca
     2161 tggagcctct caggcactgg ccaggctgcg ccgtccgaga tcgaagccgc tgtcgccgct
     2221 ccgtccgtaa gtggccggcg cggcgcgatg gccgagcagc gtgctgcgcg tgcgccgcag
     2281 ctgcagctgg cgctgttcga gcagcgcgcc gttatcctgg ttgagctgct ggcattgcgc
     2341 ggccagccgc agcagttcac gccagcgtgc gacgctggcg cccgcatcgg gcgcctgcgc
     2401 cagaagtgtc tcgatctgcg ccggcgccgc ctgcagcttg cccagctgga gacccagcgc
     2461 ctgcagcttc tgcgcagcgg cgagcttgtc ggcgcaggcc tgttccagtt cgtcgagatt
     2521 gga
//
LOCUS       DASZZC010000294         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1118139,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000294 DASZZC010000000
VERSION     DASZZC010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118139"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VHE37_06175"
     CDS             complement(<1..690)
                     /locus_tag="VHE37_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HVT35147.1"
                     /translation="MKIIRPIALTDLDALLHMAQTAGSGFTSLPPVKQYLQQKIELSL
                     HSFDAKVSRAGHERYMYVLADSDSGEVGGCCAVEAACGLDEAFYNYHVGTTVHASREL
                     QVYNRIPTLYLSNDLTGTSVLCSLYLRPEFRAGGAGRLLSRCRFMFMAQFPQRFAEKV
                     IAEMRGVSNEHGRSPFWEGLGRLFFSIEYNDAEHIVGMGNKAFIAELMPKHPIYANLL
                     PPEAQAVMGRVH"
     gene            complement(701..1912)
                     /locus_tag="VHE37_06180"
     CDS             complement(701..1912)
                     /locus_tag="VHE37_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HVT35148.1"
                     /translation="MRAHEHLLRWIDEQTPAMTAELVELASINSGSLNAAGVSAVGAR
                     LAQRYAALGAGVETIEVAPYRALGDDGQWTERPLGRATRLRLRPDAPLKIFFCGHLDT
                     VFGAAHPFQQVRRTSDDTLHGPGVADLKGGLLAMWLAIAALERSEWKEQIGWEVLLNP
                     DEELGSQSSAPLLAEAAQRNHFALVYEPAFPDGHLAGERKGSGNFNVVVRGRAAHAGR
                     DHHLGRNAIRACADFVSALDALNGTRDGLTINPGYVHGGGALNIVPDLALVKFNVRTS
                     QPDDECWLHDQLDLLLTRLNDREGIQLELSGGFTRPPKIFDEKNRKLAGLLRDCGHAF
                     DLELQFKPTGGCCDGNNLSALGLPNIDNLGVVGGEIHSDREFMRISSLPERAKLSALL
                     LLRLASGEISW"
     gene            2059..>2677
                     /locus_tag="VHE37_06185"
     CDS             2059..>2677
                     /locus_tag="VHE37_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVT35149.1"
                     /translation="MERCAALFVLLLAAAGAQAAQQQLWEQSTLNQVLKSGELRVGLE
                     AGYMPFEMRDKKGDIIGFDVDLARLMAKYLGVKVKLVNTQWDGIIPALLTDKFDLLMG
                     GMTITPERNLQVNFTDPYITIGQSVIINKKNAGEIKSYRDLNDPKYTVVTKLGTTGDI
                     AAHKYMPRAKIKEFESEADAATEVRNGRADGYVYDLPYNVVYTAQN"
BASE COUNT          472 a          837 c          912 g          456 t
ORIGIN      
        1 atgcacccgg cccatcaccg cctgcgcctc gggcggcagc aggttggcgt agatcgggtg
       61 cttgggcatc agctcggcga tgaacgcctt gttgcccatg cccacgatat gttcggcgtc
      121 gttgtactcg atggagaaga acagccggcc gagtccttcc cagaacggcg agcggccatg
      181 ctcgttggac acgccgcgca tctcggcgat gaccttttcc gcgaagcgct gcgggaactg
      241 cgccatgaac atgaagcggc agcgcgacag caggcggccg gcgccgccgg cgcggaattc
      301 cgggcgcagg tagagcgagc acagcacgct ggtgccggtc aggtcgttgg acaggtacag
      361 cgtggggatg cggttgtaga cctggagttc gcggctggcg tgcaccgtgg tgccgacgtg
      421 gtagttgtag aacgcttcgt ccagcccgca ggcggcttcc accgcgcagc agccgccgac
      481 ttcgccggag tcgctgtcgg ccagcacgta catgtagcgt tcgtggccgg cgcggctgac
      541 cttggcgtcg aacgagtgca gcgacagttc gatcttctgc tgcaggtatt gcttcaccgg
      601 cggcagcgag gtgaagccgc tgccggcggt ctgcgccatg tgcagcagcg cgtcgagatc
      661 ggtaagcgcg atggggcgga tgattttcat cttgtttttt tcaccagctg atttcgccgc
      721 tggccagccg cagcaacagc agcgccgaca gcttggcacg ttccggcagc gaggagatgc
      781 gcatgaactc gcggtcggaa tggatctcgc cgccgaccac gcccaggttg tcgatgttgg
      841 gcaggcccaa ggcagagaga ttattgccgt cgcagcagcc gccggtgggc ttgaactgca
      901 gctcgaggtc gaacgcatgg ccgcagtcgc ggagcagccc ggccagcttg cggtttttct
      961 cgtcgaagat tttcggcggg cgggtgaagc cgccggaaag ttcgagctgg atgccctcgc
     1021 ggtcgttcag gcgcgtcagc agcaggtcga gctgatcgtg cagccagcac tcgtcgtccg
     1081 gctgcgaggt gcgcacgttg aatttcacca gcgccaggtc gggcacgatg ttcagcgcgc
     1141 cgccgccatg cacgtagcct gggttgatgg tgaggccgtc gcgcgtgccg ttgagcgcgt
     1201 cgagcgcgga gacgaaatcg gcgcaggcgc ggatcgcgtt gcgccccagg tggtggtcgc
     1261 gcccggcgtg cgccgcgcgc ccgcgcacga cgacgttgaa attgccgctg cccttgcgtt
     1321 cgccggcgag atggccatcg gggaatgccg gctcgtacac cagcgcgaag tgattgcgct
     1381 gcgccgcttc ggccagcagc ggcgcagacg actgcgaacc gagttcctcg tcggggttga
     1441 gcagcacttc ccagccaatc tgctccttcc actcgctgcg ttccagcgcg gcgatggcga
     1501 gccacatcgc cagcaggccg cccttgagat cagccacgcc ggggccgtgc agcgtgtcgt
     1561 cggaggtgcg ccgcacctgc tggaacggat gcgcggcgcc gaacacggtg tcgagatggc
     1621 cgcagaaaaa aatcttcagc ggcgcatcgg ggcgcaggcg caggcgcgtg gcgcggccca
     1681 gcgggcgttc agtccactgg ccgtcgtcgc cgagcgcgcg gtagggcgcc acctcgatgg
     1741 tttccacgcc ggcgccgagc gcggcgtagc gctgcgccag gcgcgcgccg accgccgaca
     1801 cgccggcggc gttgaggctg ccggagttga tcgaagccag ctcgaccagt tccgccgtca
     1861 ttgccggcgt ctgttcgtcg atccagcgca gcaggtgttc gtgggcgcgc actgccggaa
     1921 gtttctgagg taaattggct cgcattctag cttttgcgcc tccaccgccc tgccattttc
     1981 actgttataa aaaatagtat gattattaaa tcacacaatg ttttaaaaca gtccggaggg
     2041 cggtgcggta gacgtctgtt ggagcgctgc gcggcgttat tcgttctgct gctcgcggcg
     2101 gcgggcgcac aggccgcgca gcagcagctg tgggaacagt ccacgctcaa ccaggtgctc
     2161 aagagcggcg agctgcgcgt gggcctggaa gcgggctaca tgccgttcga gatgcgcgac
     2221 aagaagggcg acatcatcgg cttcgacgtc gacctcgcgc gcctcatggc gaaatatctg
     2281 ggcgtgaagg tgaagctggt caacacgcag tgggacggca tcatcccggc gctgctcacc
     2341 gacaagttcg acctgctgat gggcggcatg accatcacgc cggagcgcaa cctgcaggtg
     2401 aacttcaccg acccgtacat caccatcggc cagagcgtga tcatcaacaa gaagaacgcc
     2461 ggcgagatca agagctaccg cgatctcaac gatccgaagt acaccgtggt gaccaagctg
     2521 ggcaccaccg gcgacatcgc cgcgcacaag tacatgccgc gggcgaagat caaggaattc
     2581 gaatcggaag ccgacgccgc caccgaggtg cgcaacggcc gcgccgacgg ctacgtctac
     2641 gatctgccgt acaacgtggt gtacaccgcg cagaacc
//
LOCUS       DASZZC010000295         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_988109,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000295 DASZZC010000000
VERSION     DASZZC010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988109"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2005)
                     /locus_tag="VHE37_06190"
     CDS             complement(<1..2005)
                     /locus_tag="VHE37_06190"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HVT35150.1"
                     /translation="MNEDQHPAVTFAWVVAFLVVFALLRDEPLGWFALAGILGFVLAQ
                     WLHTRTLHERLRERVRQLEAQLEKREALASIPPAARVDAAPAAPVATAAPRPVAVAPP
                     APRPRPIPVPPAPVPPSPFEVWLKNVIGWFTRGNPLARAGIVVLFFGGAFLAKYAAEH
                     SHFPIEARFIAIALTAVGLLAVGWRLRDSHRIYARILQGGGVAGFYLTVFAATKLYQL
                     LPPAFALALMVVVAITSALLAVAQDALALAVIGTAGGFLAPLLVNTGSSNHIALFSYY
                     AALNLGIFTVAWFRAWRSLNLLGFIFTFGIAGLFRAMSYSDDQLWSTEAFLLLYFLMY
                     VGVTVLFSLRQKTELKGYISSSLVFGVPLAAFAIQVSLVRDIEYAVAWSALGLGAFYV
                     ALAAGLQRTGRDTFALLKEAFAALGIIFASLAVPLAFDAQVTASTWAVEGAGLLWLGV
                     RQQRRLARAFGALLQIGAGVGCLIGLQHAHSQMAVANSAYLGLVMLSIAGLLSGRGLH
                     AHRDALSRYERGWSAAAAIWAALWWLGGGLREIHHWVLQEHECGAALLLLAASMPALQ
                     LLAERWRWNLLAFIAQAYAAWAVLLGVGGAARIDHPLDHLACAGWPALFAAQYFMLRR
                     QDRASPSQLPLPALHAVTAWLLALLLAWEAQWRIAQEVGGVWSS"
     gene            complement(2049..>2547)
                     /locus_tag="VHE37_06195"
     CDS             complement(2049..>2547)
                     /locus_tag="VHE37_06195"
                     /inference="COORDINATES: protein motif:HMM:NF036805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35151.1"
                     /translation="GMKFFEGLTINTYREGDVVCRWLGEPVRVLEVPGHDEGQLALMP
                     DSRAWCIVGDLIQGIGTVVIAKPEGNMRKYLASLRKIIELKPRAIFPSHGLGLGGVHR
                     LEETVKHRLAREEQIRAHLAEGKTPDQMLPLIYSDIDPRLLPLARMNIESHLDKLRED
                     GVIAA"
BASE COUNT          451 a          876 c          879 g          341 t
ORIGIN      
        1 ggctgctcca caccccgccg acttcctgcg caatgcgcca ctgcgcttcc cacgccagca
       61 gcagcgcgag cagccacgcg gtgacggcat gcagcgcggg cagcggcagc tgcgacggcg
      121 atgcgcgatc ctggcgccgc agcatgaaat attgagcggc gaacaaggcc ggccagccgg
      181 cgcaggcaag atgatcgagt ggatggtcga tgcgcgccgc gccgccaact ccgagcagca
      241 cggcccacgc ggcatacgcc tgcgcgatga acgccagcag gttccagcgc cagcgctcgg
      301 ccagcagctg cagcgcgggc atcgacgcgg cgagcagcag cagcgccgcg ccgcattcat
      361 gctcctgcag cacccagtga tggatttcgc gcaggccgcc gcccagccac cacagcgcgg
      421 cccagatcgc cgccgccgcg ctccagccgc gttcataacg ggacagtgca tcgcgatgcg
      481 catgcagccc gcgcccgctg agcaggccgg cgatgctgag catgaccagg ccgagatacg
      541 cgctgttggc caccgccatc tgcgaatgcg catgctgcag gccgatcagg cagccgacgc
      601 cggcgccgat ctgcagcagc gcgccgaacg cacgcgccag ccggcgctgc tggcgcacgc
      661 ccagccagag caggcccgcg ccctccaccg cccaggtcga agcggtgact tgcgcgtcga
      721 aggccagcgg caccgccagg ctggcgaaga tgatgccgag cgcggcaaac gcttccttga
      781 gcagcgcgaa cgtgtcgcgc ccggtgcgct gcagccccgc ggccagcgct acgtagaacg
      841 cgcccaggcc cagcgcgctc caggccacgg cgtactcgat gtcgcgcacc agcgaaacct
      901 ggatggcgaa cgcggccagc ggcaccccga acaccagcga cgacgagatg tagcccttca
      961 gttcggtctt ttgccgcagc gagaacagca cggtgacgcc gacgtacatc aggaaataca
     1021 gcagcaggaa cgcttcggtc gaccacagct ggtcgtcgct gtacgacatg gcgcggaaca
     1081 acccggcaat gccgaaggtg aagatgaagc cgagcaggtt gagcgagcgc caggcgcgga
     1141 accaggcgac ggtgaagatg ccgagattga gcgcggcgta atagctgaac aacgcgatgt
     1201 ggttgctgct gccggtgttg accagcagcg gcgcgagaaa gccgccggcg gtgccgatga
     1261 cggccagcgc cagcgcgtcc tgcgccaccg cgagcagcgc agacgtgatg gccacgacca
     1321 ccatcagcgc cagggcgaag gccggtggca gcaactggta cagcttcgtc gctgcgaaca
     1381 cggtgagata gaaaccggcc acgccgccgc cctgcaggat gcgcgcgtag atgcggtggc
     1441 tgtcgcgcag gcgccagccc accgcgagca atccgacggc ggtgagggcg atggcgatga
     1501 agcgcgcctc gatcgggaag tggctgtgct cggcggcgta cttggccagg aaggcgccgc
     1561 cgaagaacag caccacgatg ccggcgcgcg ccagcgggtt gccgcgggtg aaccagccga
     1621 tgacgttctt cagccatacc tcgaacggcg agggcggcac cggtgccggc ggaacaggaa
     1681 tgggccgcgg ccgcggtgcg ggcggcgcca ctgcaacggg cctgggcgct gccgttgcaa
     1741 ccggtgcggc gggcgcggca tcgacgcgtg cggctggagg aatgcttgca agcgcctcgc
     1801 gtttttccaa ctgtgcttcg agctggcgca cgcgctcgcg caggcgttcg tgcagcgtgc
     1861 gcgtgtgcag ccattgcgcg agcacgaagc cgaggatgcc ggccagcgcg aaccagccga
     1921 gtggttcgtc gcgcagcagt gcgaacacga cgagaaaggc gacgacccag gcgaaggtca
     1981 ccgccggatg ctggtcttca ttcatgccgt tgctcccccg cacggccgtg gagttgcgtg
     2041 attgtgccct acgcggcgat gacgccgtcc tcgcgcagct tgtccaggtg cgactcgatg
     2101 ttcatgcgcg ccagcggcag caggcgcgga tcgatgtcgc tgtagatcag cggcagcatc
     2161 tggtccggcg tctttccttc cgcgagatgc gccctgatct gctcctcgcg cgccagccgg
     2221 tgcttcactg tttcctccag ccggtgcacg ccgcccaggc ccaggccgtg cgaggggaag
     2281 atcgcgcgcg gtttcagctc gatgatcttg cgcagcgagg caagatactt gcgcatgttg
     2341 ccctcgggct tggcgatgac cacggtgccg atgccctgga tcaggtctcc gacgatgcac
     2401 caggcgcggc tgtccggcat cagcgccagc tggccctcgt cgtggcccgg cacttcgagc
     2461 acgcgcaccg gctcgcccag ccagcggcac accacgtcgc cctcgcggta ggtgttgatg
     2521 gtcaggccct caaagaactt catgccg
//
LOCUS       DASZZC010000296         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_26507,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000296 DASZZC010000000
VERSION     DASZZC010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26507"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="trpD"
                     /locus_tag="VHE37_06200"
     CDS             <1..1049
                     /gene="trpD"
                     /locus_tag="VHE37_06200"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HVT35152.1"
                     /translation="VALVKITPQEALQRTIEHREIFQDEMIELMRQIMRGEVTPAMTA
                     AIITGMRVKKETVGEIAAAAQVMREFSLKVPTPPHPHLVDVVGTGGDGLHSFNISTCA
                     MFVAAAAGARVAKHGGRSVSSSSGSADVLEALGVVIDLKPQQVAQCIEECGIGFMFAP
                     NHHPAMKNVAPVRKEMGVRTLFNILGPLTNPAGAPNILMGVFHADLVGIQVRVLEKLG
                     AKHALVVHGKDGLDEITLGADTLVGELRDGKVSEYVIHPEEYGLAMSSSRNLKVADAA
                     ASAQRVLEVLDNHVGNARNIVAFNAGAALYAANVALSIADGIVRAQQALDSGAARAKL
                     DQFVQLTRKLAGKA"
     gene            1046..>1549
                     /locus_tag="VHE37_06205"
     CDS             1046..>1549
                     /locus_tag="VHE37_06205"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HVT35153.1"
                     /translation="MSDVLEKILAVKREEIAALGRRHSLATLRELAAAQPVPRGFIRA
                     LDEKVRAGGAGVIAEVKKASPSKGLIRADFDAAWIAREYEQGGAACLSVLTDERFFQG
                     SIAALQAARAAVSLPVIRKDFLIDPLQIVEARAIGADCVLLIAAALSRAQLHELHACA
                     REQALDVL"
BASE COUNT          266 a          498 c          551 g          234 t
ORIGIN      
        1 gagtcgcact cgtgaagatc acgccgcagg aagcactcca gcgcaccatc gagcatcgcg
       61 agatcttcca ggacgagatg atcgagctga tgcgccagat catgcgtggc gaagtcacgc
      121 cggccatgac cgcggccatc atcaccggca tgcgggtgaa gaaggagacg gtgggcgaga
      181 tcgccgccgc cgcgcaggtg atgcgcgagt tctcgctcaa ggtgcccacg ccgccgcacc
      241 cgcacctggt cgacgtggtc ggcaccggcg gtgacggcct gcacagcttc aacatctcca
      301 cctgcgccat gttcgtggcg gccgcggctg gtgcgcgcgt ggccaagcac ggcggccgct
      361 cggtgtcgtc ttcgtcgggc agcgccgatg tgctggaagc gcttggcgtg gtcatcgatc
      421 tcaagccgca gcaggtggcg cagtgcatcg aggagtgcgg catcggcttc atgttcgcgc
      481 ccaaccatca cccggcaatg aagaacgtgg caccggtgcg caaggaaatg ggcgtgcgca
      541 cgctgttcaa catcctgggc ccgctgacca acccggccgg tgcgccaaac atcctgatgg
      601 gcgtgttcca cgccgacctg gtcggcatcc aggtgcgcgt gctggaaaag ctcggcgcga
      661 agcacgcgct ggtggtgcac ggcaaggatg ggctggacga gatcacgctg ggcgccgaca
      721 cgctggtcgg tgaattgcgc gacggcaagg tgagcgagta cgtgatccac cccgaggaat
      781 acgggctggc catgtcgtcc agccgcaacc tcaaggtggc ggacgcggcg gcatcggcgc
      841 agcgcgtgct cgaagtgctg gacaaccacg tcggcaatgc gcgcaacatc gtggctttca
      901 atgccggcgc agcgctgtat gcggccaacg tggcgctgtc gattgccgat ggcatcgtgc
      961 gcgcgcagca ggcgctggac agcggcgcgg cgcgcgcgaa gctcgatcag ttcgtgcagc
     1021 tcacgcgtaa gctggcgggc aaggcatgag cgacgtgctg gaaaaaatcc tcgcggtgaa
     1081 acgcgaggaa atcgcggcgc tgggccggcg gcactcgctg gcgacgctgc gcgaactggc
     1141 cgcggcgcaa cccgtgccgc gcggcttcat ccgcgcgctg gacgagaagg tgcgcgccgg
     1201 cggcgccggc gtgatcgccg aggtgaagaa ggcctcgccg agcaagggac tgatccgcgc
     1261 ggatttcgat gctgcctgga tcgcgcgcga atacgagcag ggcggcgccg cctgtttatc
     1321 cgtcttgacc gacgagcgtt tcttccaggg cagcatcgcc gccctgcagg ccgcgcgcgc
     1381 cgcggtgagc ctgccggtga tccgcaagga tttcctgatc gacccgctgc agatcgtgga
     1441 agcacgcgcc atcggcgccg actgcgtgct gctgatcgcc gcagcgctgt cgcgtgcgca
     1501 actgcacgag ctgcatgcct gcgcgcgcga acaggcgctc gacgtgctg
//
LOCUS       DASZZC010000297         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_538055,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000297 DASZZC010000000
VERSION     DASZZC010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538055"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VHE37_06210"
     CDS             complement(<1..282)
                     /locus_tag="VHE37_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HVT35154.1"
                     /translation="MSIGVLLVAHGKLGHFLLDTMSTMIGRQLPLAADVLEVRAVQAH
                     EVLVNQGKRMIERLDSGDGVLILTDAFGSTPSNIANRLSGPRAAVVAGMN"
     gene            complement(279..1175)
                     /gene="rapZ"
                     /locus_tag="VHE37_06215"
     CDS             complement(279..1175)
                     /gene="rapZ"
                     /locus_tag="VHE37_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HVT35155.1"
                     /translation="MQLVIVSGLSGAGKSVALNQYEDLGYYCIDNIPLALVGPMALRA
                     LNAVEQRYALLAIGIDARESPKEIAKFPKYLEKLRARGVATRVLFLTASEPVLLKRYS
                     ETRRKHPLSNAGTSLHEAITQEGELLEPIANLADTVIDTSSLNLHELREKIMGEVPGG
                     GAGKLAVTLMSFGYKNGTPDDADFVFDVRCLPNPHWEPTLRDMGGTDFAVAAWLERHA
                     EVRRMQNDIAHFLEHWLPEYKKQDRAYLTIAIGCTGGKHRSVYLVEKLAATLRPRFEP
                     IIVKHRELWTGPDRRQSARSHA"
     gene            complement(1186..1761)
                     /locus_tag="VHE37_06220"
     CDS             complement(1186..1761)
                     /locus_tag="VHE37_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35156.1"
                     /translation="MPKRAAPAAGRPELITLHGVFMQVHGVGVLLSGGSGVGKSELAL
                     ELLTRGHQLIADDAAEFAARGKFLLGRCPPLLAGFLEARSLGILNVRRLFGRRAVARN
                     HWLHLVVHLEMRSERIAVPPALERLHGRRGTRRILDIDVPEISIPIRLGHNLAVLVEA
                     ACRDYKLRRRGYRADDDLAARQLKAIADTKP"
     gene            complement(1866..2324)
                     /locus_tag="VHE37_06225"
     CDS             complement(1866..2324)
                     /locus_tag="VHE37_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HVT35157.1"
                     /translation="MNLTEILAKDRVASGLTFTSKKKALEELSGLLAKGSGLPAADIL
                     GALAAREKLGSTGLGHGVAIPHGRMAGVESSVGAFMRLKHPLDYDAHDGNGVDLVFGL
                     LVPQNASETHLKHLAAIAEMFSDEAFCKKMRGAADDGALYELLHGYKSAS"
     gene            complement(2408..2728)
                     /gene="raiA"
                     /locus_tag="VHE37_06230"
     CDS             complement(2408..2728)
                     /gene="raiA"
                     /locus_tag="VHE37_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVT35158.1"
                     /translation="MNLNISGHHVELTPPLREYVKDKLTRVERHFDHLISAEVILSVE
                     KLRQKAEATIHASGANMHAEAVDGDMYAAIDLLMDKLDQQTRKHKEKLRDHHHKEAHK
                     RMAQ"
     gene            complement(2743..>3079)
                     /locus_tag="VHE37_06235"
     CDS             complement(2743..>3079)
                     /locus_tag="VHE37_06235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456520.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; sigma 54 factor is
                     responsible for the expression of enzymes involved in
                     nitrogen assimilation and metabolism; the rhizobia often
                     have 2 copies of this sigma factor; in Rhizobium etli
                     RpoN1 shown to be involved in the assimilation of several
                     nitrogen and carbon sources during free-living aerobic
                     growth and RpoN2 is involved in symbiotic nitrogen
                     fixation; in Bradyrhizobium both RpoN1 and N2 are
                     functional in free-living and symbiotic conditions, rpoN1
                     gene was regulated in response to oxygen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HVT35159.1"
                     /translation="SEQLGIHESTVSRATANKYMLTPRGLFELKYFFSSHVATTAGGV
                     ASATAIQAMIKRLIAGEDPAKPLSDSTLAEVLLKEGIQVARRTVAKYREGLNIPPSHE
                     RKPPVTSRL"
BASE COUNT          460 a          970 c         1058 g          591 t
ORIGIN      
        1 gttcatgccg gccaccaccg cggcgcgcgg gccggacagg cggttggcga tgttgctcgg
       61 ggtggagccg aaggcgtcgg tgaggatcag cacgccgtcg ccggagtcga gccgctcgat
      121 catgcgcttg ccctggttga ccagcacctc gtgcgcctgc accgcgcgca cttccagcac
      181 gtcggccgcc agcggcagct gcctgccgat catggtgctc atggtgtcga gcaggaaatg
      241 cccgagcttg ccgtgcgcca ccagcagcac gccgatgctc atgcgtggct ccgcgcggac
      301 tggcgccggt ccgggccggt ccacagctcg cggtgcttga cgatgatcgg ctcgaagcgc
      361 gggcgcagcg tggccgccag cttctccacc aggtacacgc tgcggtgctt gccgccggtg
      421 cagccgatgg cgatggtcag gtaggcgcgg tcctgcttct tgtattcggg cagccagtgc
      481 tccaggaaat gcgcgatgtc gttctgcatc ctgcgcacct cggcgtggcg ctcgagccag
      541 gcggcaacag cgaaatcggt gccgcccatg tcgcgcagcg tcggctccca gtgcgggttg
      601 ggcaggcagc gcacgtcgaa cacgaaatcg gcgtcgtccg gcgtgccgtt cttgtagccg
      661 aagctcatca gcgtgaccgc gagtttcccc gcgccgccgc cgggcacttc gcccatgatc
      721 ttctcgcgca gttcgtgcag gttgagcgag gaggtgtcga tgacggtgtc ggccaggttg
      781 gcgatcggct ccagcagctc gccctcctgc gtgatcgcct cgtgcagcga ggtgccggca
      841 ttggacaggg ggtgcttgcg ccgcgtttcc gagtagcgct tgagcagcac cggctcgctg
      901 gcggtgagga acagcacgcg cgtggccacg ccgcgcgcgc gcagcttctc caggtacttg
      961 ggaaacttgg cgatctcctt cgggctctcg cgcgcgtcga tgccgatggc cagcagcgcg
     1021 tagcgctgct ccacggcatt caatgcgcgc agcgccatcg gcccgaccag cgccagcggg
     1081 atgttgtcga tgcagtaata gcccaggtct tcgtactggt tgagcgccac cgatttcccg
     1141 gcgccggaca atccactgac gatgaccagc tgcatggcgg cgtatttagg gcttggtgtc
     1201 tgcgatggcc ttgagctggc gcgcggcgag gtcgtcgtcg gcgcggtatc cgcggcgccg
     1261 cagcttgtag tcgcggcagg cggcctccac cagcacggcc agattatgcc cgagccggat
     1321 cgggatggag atttccggca cgtcgatgtc caggatgcgg cgcgtgccgc ggcgcccgtg
     1381 caggcgctcc agcgccggcg gcaccgcgat gcgctcgctg cgcatctcca ggtgcaccac
     1441 caggtgcagc cagtggttgc gcgccacggc gcggcggccg aacaggcgcc gcacgttgag
     1501 gatgcccagc gagcgcgcct cgagaaaacc ggccagcagc ggggggcagc ggccgagcag
     1561 gaattttccg cgcgcggcga attcggcggc atcgtcggcg atcagctggt ggccgcgcgt
     1621 gagcagttcc agcgccagtt cgctcttgcc cacgccgctg ccgccggaca gcaacacacc
     1681 cacgccatgc acctgcatga acacgccgtg cagcgtgatc agctcgggcc tgccggcagc
     1741 gggagcggcg cgttttggca tctaccgcac tggcattttg gatgttatat aaaattgtac
     1801 gaaagttaaa tcgtacatca ttttataaca atgaaaacgg cgctgcggta tagggatttt
     1861 tgcgctcagg aagcggactt gtagccgtgc agcagttcgt acagcgcgcc atcgtcggcg
     1921 gcgccgcgca tcttcttgca gaaggcttcg tcggaaaaca tctcggcgat ggcggccagg
     1981 tgcttgaggt gcgtttcgga ggcgttctgc ggcaccagca ggccgaacac caggtcgacg
     2041 ccgttgccgt cgtgcgcgtc gtagtccagc gggtgcttca ggcgcatgaa ggcgccgacc
     2101 gacgattcca cgccggccat gcgcccgtgc gggatggcca cgccgtggcc caggccggtg
     2161 ctgcccagct tctcacgcgc ggcgagcgcg ccgaggatgt cggcagcggg caggccggag
     2221 cccttggcca gcaggccgga caattcctcc agcgcttttt tcttgctggt gaaggtgagg
     2281 ccgctggcta cgcggtcctt ggcgaggatt tcggtcaggt tcatggtctt caggttctcg
     2341 gtatcgtggc ccgcttgaag caaaaggccc agccatcgct ggctgggccg gtggcgtggc
     2401 gcgaatttta ctgcgccatg cgcttgtgcg cttccttgtg gtgatggtcg cgcagtttct
     2461 ccttgtgctt gcgcgtctgc tggtcgagct tgtccatcaa caggtcgatg gccgcgtaca
     2521 tgtctccgtc caccgcttcg gcgtgcatgt tcgcgccgct ggcgtggatt gtcgcctccg
     2581 ccttctgccg cagcttctcc acggaaagga ttacttcggc gctgatcagg tggtcgaaat
     2641 ggcgttccac gcgggtcagc ttgtccttca cgtattcgcg cagcggcggg gtgagttcga
     2701 cgtgatgtcc ggagatgttc aggttcatcg agtgctccta cgttagagcc ggctggtgac
     2761 cggcggtttc ctctcgtgcg agggagggat gttgagtccc tcccggtact tggccaccgt
     2821 gcggcgcgcc acctggatgc cttctttcaa aagcacctcg gcgagcgtgg agtcggacag
     2881 cggcttggcc gggtcttccc cggcgatgag gcgcttgatc atggcctgga tggccgttgc
     2941 ggaggccacg ccaccggcgg tcgtggccac atggctggaa aagaaatact tgagctcgaa
     3001 cagcccacgt ggcgtgagca tgtacttgtt ggccgtggct cgcgaaaccg tggattcatg
     3061 gatccccagt tgttccgat
//
LOCUS       DASZZC010000298         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_473584,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000298 DASZZC010000000
VERSION     DASZZC010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473584"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VHE37_06240"
     CDS             <1..480
                     /locus_tag="VHE37_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT35160.1"
                     /translation="IADDVPLIHSLIRELAEYEKLSQEVSATQDALRAHLFGAQRYAE
                     TLIAEVNGAAAGFALYFHNYSTFLARPGIYLEDLFVRPAARRRGAGKALLVELARIAV
                     ERGCGRLEWAVLDWNEPAIAFYRSLGAQMMGDWRIKRVTGEALRVLAAGGPAPANRP"
     gene            488..>3013
                     /gene="putA"
                     /locus_tag="VHE37_06245"
     CDS             488..>3013
                     /gene="putA"
                     /locus_tag="VHE37_06245"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HVT35161.1"
                     /translation="MSKIETARIAFVHSTLPPARDRLRAAISQNYLADETQLVDYLAG
                     IAELAPEESLDTCRTAAHWVSRVRMRRDQQSPLDAFMRQYDLSSEEGVLLMCLAEALL
                     RIPDAATAEKLIADKLGGADWDRHIGRSDSLFVNASTWGLMLTGRIVKLSATTTSNVR
                     AAFGTLINKSSEPVIRLAVRQAMKLMGHQFVMGRTIEEALRNSKSREHLAYLHSFDML
                     GEAALTADDARRYFDAYTAAIAAIGAHAKLDHGGSVFLRPSISIKLSALHPRYEYAKR
                     ERVLAELTPRLLALAHQARKENIGLTIDAEEADRLELSLDLFEQVYRDRTLSGWEGLG
                     LAVQAFSKRALFVIEWLEALGRSVGRLIPVRLVKGAYWDTEVKRSQEQGLASYPVFTR
                     KVNTDVAYLACARRLLAARDVFYPQLATHNAHTVAAVKAYAGTSGGYEFQRLHGMGEA
                     LYQVVREELPHPVRIYAPVGSHEDLLPYLVRRLLENGANTSFVNRVFDEAVPPEQLAA
                     DPVAAVIAARGRVNTALPSPRELYGAERRNSAGINLASEPEAAALAQALTAAAVPRRA
                     VSLLAPGIDNAGEARPVTDPSDRRRVLGEWVALPPSRANEVIAAASAAYPNWNATSVG
                     ERATLLENCADALEARRAEFMHLLIREAGKTIPDAIAEVREAVDFLRYYAAQARSLMG
                     LPEILAGPTGEHNELQLHGRGVFVCISPWNFPLAIFTGQLAAALVTGNTVIAKPAEQT
                     NLVAMQMVQLMHDAGVPEEALQCVLGAGDLGAALTAHDGIAGVAFTGSVEVAQAINRA
                     LAARKGPIATLIAETGGQNAMIADSSALPEQIVKDVIASAFQSA"
BASE COUNT          479 a         1047 c         1040 g          447 t
ORIGIN      
        1 attgctgacg acgtaccgct gatccactcg ctgatccgcg agctcgcgga gtacgagaag
       61 ctgtcgcagg aagtcagcgc gacgcaagac gcattgcgcg cacacctgtt cggcgcgcag
      121 cgctatgcgg aaacgctgat cgcggaagtg aacggcgcag cggccgggtt cgcgctgtac
      181 ttccacaact actcgacctt cctggcgcgg ccggggatct atctcgaaga tctgttcgtg
      241 cggccggccg cgcgccgccg tggcgccggc aaggccttgc tggtggaact ggcgcgcatc
      301 gcggtggagc gcggctgcgg ccggctggaa tgggcggtgc tcgactggaa cgaaccagcc
      361 atcgccttct accgcagcct cggcgcgcag atgatgggcg actggcgcat caagcgcgtc
      421 accggcgagg ccctgcgcgt actggcggcg ggcggacccg cgccggcaaa tcggccatga
      481 tgtgtccatg agcaagatcg aaaccgcacg gattgctttc gtccattcca cgctgccgcc
      541 cgcgcgcgac cgcctgcgcg ccgcgatctc gcagaactac ctggcggacg agacgcagct
      601 ggtcgactac ctggccggca ttgccgagtt ggcgccggaa gagagcctcg acacctgccg
      661 caccgcggcg cactgggtgt cgcgcgtgcg catgcgccgc gaccagcagt cgccgctcga
      721 cgccttcatg cgccagtacg acctgtcgag cgaggagggc gtgctgctga tgtgcctggc
      781 cgaggcgctg ctgcgcatcc ccgacgcggc caccgccgag aagctgatcg ccgacaagct
      841 gggcggcgcc gactgggacc gccacatcgg ccgcagcgat tcgctgttcg tcaacgcctc
      901 cacctggggg ctgatgctga cgggccgcat cgtgaaattg tcggccacca cgaccagcaa
      961 cgtgcgcgcg gcgttcggca cgctgatcaa caagtcgagc gaaccggtga tccgcctggc
     1021 cgtgcgccag gcgatgaagc tgatgggcca ccagttcgtc atggggcgca ccatcgagga
     1081 ggcgctgcgc aattcaaagt cgcgcgagca cctggcctac ctgcattcgt tcgacatgct
     1141 gggcgaggcg gcgctgaccg ccgacgatgc ccggcgctac ttcgatgcct ataccgccgc
     1201 catcgcggcg atcggcgcgc atgccaagct cgatcacggc ggctcggtgt tcctgcggcc
     1261 gtcgatctcg atcaagctgt ccgcgctgca cccgcgctac gaatacgcca agcgcgagcg
     1321 cgtgctggcc gagctgacgc cgcgcctgct cgcgctggcg caccaggcgc gcaaggagaa
     1381 catcgggctg acgatcgatg ccgaggaagc cgaccgtctg gaactgtcgc ttgacctgtt
     1441 cgagcaggtc taccgcgacc gcacgctgtc cggttgggaa ggcctgggcc tcgccgtgca
     1501 ggcgttctcc aagcgcgcgc tgttcgtcat cgaatggctg gaagcgctgg gccgcagcgt
     1561 cggccggctg atcccggtgc gcctggtcaa gggtgcgtac tgggacaccg aggtcaagcg
     1621 ctcgcaggag cagggcctgg ccagctaccc ggtgttcacg cgcaaggtga ataccgacgt
     1681 ggcctatctc gcctgcgcgc gcaggctgct ggccgcgcgc gacgtgttct acccgcagct
     1741 ggccacgcac aacgcgcaca ccgtcgccgc ggtgaaagcg tatgcaggca ccagcggcgg
     1801 ctacgagttc cagcgcctgc acggcatggg cgaggcgctg taccaggtgg tgcgcgaaga
     1861 actgccgcac ccggtgcgca tctacgcgcc ggtcggctcg cacgaagacc tgctgccgta
     1921 cctggtgcgg cgcctgctgg agaacggcgc caacacctcg ttcgtcaacc gcgtgttcga
     1981 cgaggccgtg ccgcccgagc aactggccgc cgacccggtg gccgcggtca tcgccgccag
     2041 gggccgcgtc aacaccgcgc tgccgtcccc acgcgagctg tacggagccg agcgcaggaa
     2101 ttccgccggc atcaacctcg cgtccgaacc ggaggcggct gcgctggcgc aggcgctcac
     2161 cgccgccgcg gtgccgcgcc gcgcggtgtc gctgctggcg cccggcatcg acaacgccgg
     2221 cgaggcgcgg ccggtgaccg acccgtctga tcgccgccgc gtgctcggtg aatgggtggc
     2281 cttgccgccg tcgcgcgcga acgaagtgat cgccgcagcg agtgccgcct acccgaactg
     2341 gaacgccacc tcggtgggcg agcgcgccac gctgctggag aactgcgccg atgcgctgga
     2401 agcgcgccgc gcggagttca tgcacctgct gatccgcgag gccggcaaga cgatacccga
     2461 cgcgatagcc gaggtgcgcg aagcggtcga tttcctgcgc tactacgcgg cgcaggcgcg
     2521 ttcgctgatg ggcctgccgg aaatcctggc cggccccacc ggcgagcaca acgaactgca
     2581 gctgcacggg cgcggcgtgt tcgtctgcat cagcccgtgg aattttccgc tggcgatctt
     2641 caccgggcag ctggccgccg cgctggtcac cggcaacacg gtgatcgcca agccggccga
     2701 gcagaccaat ctggtcgcca tgcagatggt gcagctgatg cacgatgccg gcgtgccgga
     2761 agaggcgctg caatgcgtgc tgggcgcggg cgatctcggt gctgcgctga ccgcgcacga
     2821 tggaattgcc ggcgtggctt tcaccggctc ggtggaagta gcgcaggcga tcaaccgtgc
     2881 gctcgctgca cgcaaggggc cgatcgccac gctgatcgcc gagaccggcg gccagaacgc
     2941 gatgatcgcc gactcctccg ccctgcccga gcagatcgtc aaggacgtga tcgcctccgc
     3001 cttccagtcc gcc
//
LOCUS       DASZZC010000299         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_926160,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000299 DASZZC010000000
VERSION     DASZZC010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926160"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VHE37_06250"
     CDS             complement(<1..564)
                     /locus_tag="VHE37_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT35162.1"
                     /translation="MLLLAGCGGHAEAPPGPRPVLVEHPQPLAGEAGEAFPGTVRARV
                     EADLSFRVPGKISKRLVDAGASVKPGQVLASLDPQDAHLNIAAAGAAVVAAEADVKLA
                     EAQLTRQKEMIARGFASQATLDVQENQAQLARAKAGQARAQLAVVKNQAVYSDLVADR
                     AGTITAVQAETGQVVAAGQPVFQFAAQG"
     gene            666..1313
                     /locus_tag="VHE37_06255"
     CDS             666..1313
                     /locus_tag="VHE37_06255"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT35163.1"
                     /translation="MKTPTIPRRRGRPKDPAKRSAILEAAKDLFARHGLAGTSMEAIA
                     ARAGVSKLTLYSHFATKDDLFQQAVIAKCEEHAPAALFEGKPGEPLRARLTGIAGGFL
                     ALVMNEQAMELHRMMCAQAGSDRRLGRLFFSAGPQRTIAQFARLLAAATRSGELAVPD
                     PQRAAAHFFCLLKGADHLRVMVGAGRAPPPPQTRAHIADVVELFLRAYAPAAKRR"
     gene            complement(1461..>2767)
                     /locus_tag="VHE37_06260"
     CDS             complement(1461..>2767)
                     /locus_tag="VHE37_06260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVT35164.1"
                     /translation="HSVLANGAQLLLMERHDVPLISMTLTLAGGPLADEPGREGSAAL
                     LADLMEKGAGARNAAEWAEAVAAAGGEIDIGATRESLQLSAEFLARDSALMLELASDA
                     LLRPRLDAGEFDKLRQRTIDELAADKDGDPGALLGFYGYAWLLGNSGYGRSYIGDETT
                     LKQITLADLQRYYRQQLGGDRLIIAIAGDFNAAALAPQVERAFGGWRKAELPLQAPVA
                     AQRQHGRRVLLVDKPGATQSYFWIGNVGTTHNDPARVPQELVNEIFGGRYTSMLNSEL
                     RIKSGLSYSAGSRIARLRQPGPLYVQSFTATETTRRAIDLALQTLARLHREGVSPAAL
                     KSTQDYVIGSFPPEFETASQLSAHLAELAFHGLGRDEVDDYAARVRAVTSAQARQVVD
                     QLYPPAEDVAMVVIGDAAKIRGELASYGAVTEMKMSAPRFAP"
BASE COUNT          420 a          993 c          909 g          445 t
ORIGIN      
        1 gccctgtgcg gcgaactgga acaccggctg gccggccgcg accacctggc ccgtttccgc
       61 ctgcacggcg gtgatcgtgc ccgcgcggtc ggctacaagg tccgagtaca ccgcctggtt
      121 cttcaccacg gcgagttgcg cgcgcgcctg gccggccttg gcccgcgcca gctgcgcctg
      181 gttctcctgc acgtcgagcg tggcctggct ggcaaagccc ctggcgatca tttccttctg
      241 ccgcgtgagc tgcgcctcgg ccagcttcac gtcggcctcg gcggcgacca ccgcggcgcc
      301 cgcggcggcg atgttgaggt gggcatcctg cggatcgagc gaggccagca cctgcccggg
      361 tttgaccgag gcgccggcat cgaccaggcg cttgctgatt tttccgggca cgcggaacga
      421 caggtccgct tccacccgcg cgcgcaccgt gccgggaaag gcctcgccgg cttcgcccgc
      481 cagcggctgc ggatgttcca ccagcacagg ccgcgggccg ggcggcgcct cggcgtgacc
      541 gccgcagccc gcgagcagca gcacacccgc cgcgcctgtc gccaaaagga aatgccgcat
      601 gccgcccccg tgccgaaata acatactgaa cggtatggta tagaaattcc gctaccatct
      661 ccaccgtgaa aaccccgacc atcccccgcc gccgcggccg gccgaaagat ccggccaagc
      721 gcagcgccat tctcgaagcg gccaaggacc tgttcgcgcg tcacggcctg gccggcacca
      781 gcatggaggc gatcgccgcg cgcgccggcg tatccaagct cacgctgtac tcgcacttcg
      841 ccaccaagga cgacctgttc cagcaggcgg tgatcgccaa gtgcgaggag catgcgcctg
      901 cggccctgtt cgaaggcaag cccggcgagc cgctgcgcgc gcggctcacg ggcatcgccg
      961 gcggcttcct cgcgctggtg atgaacgagc aggcgatgga actgcaccgc atgatgtgcg
     1021 cgcaggccgg cagcgatcgg cggctgggca ggctgttctt ttccgccggg ccgcagcgga
     1081 ccatcgccca gttcgcacgc ctgctcgccg cagccacgcg cagcggcgaa ctcgcggtgc
     1141 ctgatccgca gcgcgccgcc gcgcatttct tctgcctgct caagggcgcc gatcacctgc
     1201 gcgtgatggt gggcgccggc cgcgcgcccc cgcccccgca gacgcgcgcg catattgccg
     1261 acgtggtgga actgttcctg cgcgcctacg cgcctgccgc gaagcgccgc tgaactgaat
     1321 tgaacccgct ggtcggcaaa aatgcctaca ccgcaccgcc ggttttgatg ttattaatta
     1381 ttgcatataa ttaaaaacat aaaatatttt ataacaggca aaatgcaggt gcggtcagcc
     1441 cgtttaggcg ccgtaccaac tcacggcgcg aagcgtggcg cgctcatctt catttccgtg
     1501 accgcgccat acgatgcgag ctcgccgcgg attttcgccg catcgccgat caccaccatc
     1561 gccacgtctt cggccggcgg atagagctgg tcgaccacct gccgcgcctg cgcggaagtc
     1621 accgcacgca cgcgcgccgc gtaatcgtcc acttcgtcgc ggcccagccc gtgaaacgcc
     1681 agctcggcga gatgcgccga cagctggctg gccgtctcga actccggcgg gaagctgccg
     1741 atgacgtagt cctgggtgga tttcagcgcg gcgggcgaca cgccttcacg atgcaggcgc
     1801 gcgagcgtct gcagggcgag atcgatggcg cgccgcgtcg tttcggtggc ggtgaacgat
     1861 tgcacataca gcggccccgg ctggcgcagc cgcgcgatgc gcgagccggc gctgtacgac
     1921 agcccgctct tgatgcgcag ttcgctgttg agcatggagg tgtagcggcc gccaaagatt
     1981 tcgttgacca gttcctgcgg cacgcgcgcg ggatcgttgt gcgtggtacc gacgttgccg
     2041 atccagaaat agctctgcgt ggcgccgggc ttgtccacca gcagcacgcg ccggccgtgc
     2101 tgccgctgcg ccgcgaccgg cgcttgcagc ggcagttcgg ccttgcgcca gccgccgaac
     2161 gcgcgctcga cctgcggcgc cagcgcggcc gcattgaaat cgccggcgat ggcgatgatc
     2221 agccggtcgc cgcccagctg ctgacggtag tagcgctgca ggtcggccag cgtgatctgc
     2281 ttcagggtcg tctcgtcgcc gatgtagctg cggccgtagc cgctgttgcc gagcagccac
     2341 gcatagccgt agaagccgag cagcgcgccg ggatcgccat ccttgtccgc cgccagctcg
     2401 tcgatggttc gctgacgcag cttgtcgaat tccccggcgt ccagccgcgg gcgcagcagc
     2461 gcgtcggagg ccagttccag catgagggcg gaatcgcgcg ccaggaattc cgccgacagc
     2521 tgcaggcttt cgcgcgtggc accgatgtcg atctcgccgc ccgccgccgc caccgcctcg
     2581 gcccattccg ccgcgttgcg cgcgccggcg cccttctcca tcaggtcagc gagcagcgcc
     2641 gcgctgcctt cgcggccggg ttcgtcggcc agcggtccgc cggccagcgt caacgtcatg
     2701 ctgatcagag gtacgtcgtg acgttccatc aggagcagtt gcgcgccgtt ggccagcacg
     2761 ctgtgcg
//
LOCUS       DASZZC010000300         3478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1243619,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000300 DASZZC010000000
VERSION     DASZZC010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243619"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VHE37_06265"
     CDS             <1..236
                     /locus_tag="VHE37_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVT35165.1"
                     /translation="AYLGAADAALQARVLRSLAGSGVLTVYDAAERRPGGAIRMFVED
                     QRMRFEVNTAVVEREHLELSSRLLGVAQLVRDP"
     gene            233..1798
                     /locus_tag="VHE37_06270"
     CDS             233..1798
                     /locus_tag="VHE37_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35166.1"
                     /translation="MSSGESSIRGKLTALSVLSSAAGLLLAAAALIIYAWVTVRAGYQ
                     RDLQTVAHIVADNSTAALVFGDRRAASETLAALRAKPEIQLACIYDGGGSAFGSYAAG
                     SVQACPPRPETPGIYRDTEALTLVDEVRLKGERVGLLRLTQTLQPLRDTLENQVAITL
                     SIMAVSFFLSLSIAWLMQRGISGPIVDLAQTARRVSESRDYSLRAGGSGSRDEVGLLV
                     EAFNQMLGQIAQRDGQLRQARDELAQEVGEKTQANTELQAALLRLREAQAQLVQSEKL
                     ASLGALVAGVAHEINTPIGIGVTASSTLQARAAQVTQKYKDETLKRSDLEGFIATADE
                     STRIILKNLQRAADLIHSFKQVAVDQTSGERRRFGLKSYIDEVLLSLGPRLKATGHSV
                     LVDCPGDLVLDSYPGALAQILTNFVSNSLMHAFDTGVDGRMEISVRESQGMVELRYAD
                     NGRGMPPADLARIFDPFFTTKRGAGGSGLGMHVVYNLVTQLLKGQISVASEPGHGVEF
                     LLRFPVESAKGAT"
     gene            1795..2730
                     /locus_tag="VHE37_06275"
     CDS             1795..2730
                     /locus_tag="VHE37_06275"
                     /inference="COORDINATES: protein motif:HMM:NF023277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HVT35167.1"
                     /translation="MNDELKFADEGGQAATPARAPWTLLIVDDEQEVHAVTTLALREF
                     SFNGKGVRFLHAYSGAQAQRMLADHPEIALVLLDVVMETDQAGLDVVEYVRNQLKNPF
                     VRIVLRTGQPGQAPELEVIRRYDINDYKHKTELTRERLFTTVYTGLSTYRDLMALDAN
                     RRGLEKVIDATAHILELRSLEHFAQGVLEQLTALLFIERGALVVQISGVAAESNGGLS
                     ILAGTGVYQALAGRDARSVLPADVLARIEAARVAQKTLYGADYFTGYEHHLIFDIGAD
                     QPISLPDRRLIELFLRNVGIARDNLRLLHERHAAG"
     gene            2717..>3478
                     /locus_tag="VHE37_06280"
     CDS             2717..>3478
                     /locus_tag="VHE37_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVT35168.1"
                     /translation="MPPGDTDLLGLRASSAPDAPPWLVLVVDDDAEVHAVTRLALSAF
                     EFAGRPVRILEAHSAAEARTRLAQHTDIALILLDVVMETEHAGLDLVRYIRDELKNSI
                     VRIVLRTGQPGQAPPLDVVVRYEIDDYRSKTEMTFERLNISTLTALRAYRLLQQMEQR
                     QRELAQSNQDLERFAYVASHDLQAPLRSVAGFAQILQKRYTMALDSEGRELLDFVMRG
                     TSDMQALINDLLQLSRVGRSSAARGLVNLNQVLSKV"
BASE COUNT          579 a         1202 c         1196 g          501 t
ORIGIN      
        1 tggcctatct cggcgctgcg gacgcagcgc tgcaggcgcg cgtcctgcgc agcctggccg
       61 gcagcggcgt gctgaccgtc tacgatgcgg cggagcggcg cccgggcggc gccatccgca
      121 tgttcgtcga agaccagcgc atgcgcttcg aggtcaacac cgcggtggtc gagcgcgaac
      181 acctggaact gagctcgcgc ctgctcggcg tggcgcagct ggtgagggac ccatgagttc
      241 cggcgaaagc tcgatccgcg gcaagctcac cgcgctcagc gtgctgtcga gcgcggccgg
      301 cctgctgctg gccgcagccg cgctgatcat ttatgcctgg gtcacggtgc gcgccggcta
      361 ccagcgcgac ctgcagacgg tggcgcatat cgtggccgac aactcgactg ccgcactggt
      421 gttcggcgac cgccgcgcgg cttcggaaac cctggcggcg ctgcgcgcca aacccgagat
      481 ccagctggcc tgcatttacg atggcggcgg cagcgccttc ggcagctacg ccgccggcag
      541 tgtgcaggcc tgcccgccgc gcccggaaac gccgggcatc taccgcgaca ccgaggcgct
      601 gacgctggtc gacgaggtgc gcctgaaagg cgagcgcgtc ggcctgctgc gcctgacgca
      661 gacgctgcag ccgctgcgcg acacgctgga gaaccaggtt gccataaccc tgtcgatcat
      721 ggcggtgagc ttcttcctca gcctgtcgat cgcctggctg atgcagcgcg gcatcagcgg
      781 tccgatcgtc gacctggcgc agaccgcgcg gcgcgtgtcg gaatcgcgcg actacagcct
      841 gcgcgccggc ggcagcggca gccgcgacga ggtcgggctg ctggtcgagg ccttcaacca
      901 gatgctcggg cagatcgcgc agcgcgacgg ccagctgcgc caggcgcgcg acgagctggc
      961 gcaggaagtg ggcgagaaga cccaggccaa caccgagctg caggcggcgc tgctgcgcct
     1021 gcgcgaggcg caggcgcagc tggtgcagag cgagaagctg gcctcgctcg gcgcgctggt
     1081 ggccggcgtg gcgcatgaga tcaacacgcc aatcggcatc ggcgtcaccg cctcgtccac
     1141 gctgcaggcg cgggcggcgc aggtcacgca gaaatacaag gacgagacgc tcaagcgctc
     1201 cgacctggaa ggcttcatcg ccaccgccga cgagtccacc cgcatcatcc tgaagaacct
     1261 gcagcgcgcg gccgacctga tccacagctt caagcaggtg gcggtggacc agaccagcgg
     1321 cgagcgccgc cgcttcgggc tcaagtcata catcgacgag gtgctgctgt cgctgggccc
     1381 gcggctcaag gccaccggcc acagcgtgct ggtcgactgc cccggcgacc tggtgctgga
     1441 cagctatccc ggcgcgctgg cgcagatcct gaccaacttc gtcagcaact cgctgatgca
     1501 cgcgttcgac accggcgtcg acgggcgcat ggaaatcagc gtgcgcgaga gccagggcat
     1561 ggtcgagctg cgctatgccg acaacggccg cggcatgccg ccggccgacc ttgcgcgcat
     1621 cttcgatccc ttcttcacca ccaagcgcgg tgccggcggc agcggcctgg gcatgcacgt
     1681 ggtctacaac ctggtaacgc agctgctcaa ggggcagatc agcgtggcca gcgagccggg
     1741 ccacggcgtc gaattcctgc tgcgtttccc ggtcgaatcc gccaaggggg cgacatgaac
     1801 gacgaactga aattcgccga cgagggcggc caggccgcca cgccggcgcg cgcgccatgg
     1861 accctgctga tcgtcgacga cgagcaggaa gtgcacgcag tgaccacgct ggcgctgcgc
     1921 gagttctcgt tcaacggcaa gggcgtgcgc ttcctgcacg cctactccgg cgcgcaggcg
     1981 cagcgcatgc tggcagacca cccggaaatc gcgctggtgc tgctcgacgt ggtgatggaa
     2041 accgaccagg ccgggctcga cgtggtcgaa tacgtgcgca accagctgaa gaatcccttc
     2101 gtgcgcatcg tgctgcgcac cggccagccc gggcaggcgc cggaactgga agtgatccgc
     2161 cgctacgaca tcaacgacta caagcacaag accgagctga cgcgcgagcg cctgttcacc
     2221 acggtgtaca ccggcctgtc gacctaccgt gacctgatgg cgctggatgc caaccggcgc
     2281 ggactggaaa aagtgatcga cgccaccgcg cacatcctcg aactgcgctc gctggagcac
     2341 tttgcccagg gtgtgctgga gcagctgact gcgctgctgt tcatcgagcg cggcgcgctg
     2401 gtggtgcaga tctccggcgt ggctgcggaa agcaatggcg ggctgagcat cctggccggc
     2461 accggcgtgt accaggcgct ggccgggcgc gacgcgcgca gcgtgctgcc ggccgacgtg
     2521 ctggcgcgca tcgaggccgc gcgcgtggcg cagaagacgc tgtacggggc ggactacttc
     2581 accggctacg aacatcacct gatcttcgac atcggtgccg accagccgat cagcttgccg
     2641 gaccgccggc tgatcgaact gttcctgcgc aacgtcggca tcgcccgcga caatctccgc
     2701 ctgctgcacg aacgccatgc cgccgggtga taccgacctg ctgggcctgc gggcgtcgag
     2761 cgcgcccgat gcgccgccgt ggctggtgct ggtggtcgac gacgatgccg aggtgcatgc
     2821 cgtcacccgc ctggcgctgt cggccttcga gttcgccggc cggccggtgc gcatcctcga
     2881 ggcgcacagc gcggccgagg cgcgcacgcg gctggcgcag cacaccgaca tcgcgctgat
     2941 cctgctcgac gtggtgatgg agaccgagca tgccgggctc gacctggtgc ggtacatccg
     3001 cgacgagctg aagaattcca tcgtgcgcat cgtgctgcgc accggccagc cggggcaggc
     3061 gccgccgctg gacgtggtgg tgcgctacga gatcgacgat taccgcagca agaccgagat
     3121 gaccttcgag cgcctgaaca tctcgacgct caccgccctg cgcgcctatc gcctgctgca
     3181 gcagatggag cagcgccagc gcgagctggc gcaatccaac caggacctcg agcgcttcgc
     3241 ctacgtggcc tcgcacgacc tgcaggcgcc gctgcgcagc gtggccggct tcgcccagat
     3301 cctgcagaag cgttacacca tggcgctcga cagcgaaggc cgcgaactgc tcgatttcgt
     3361 catgcgcggc accagcgaca tgcaggcgct gatcaacgat ctgctgcagc tgtcgcgggt
     3421 cggccgcagc agcgccgcac gcggcctggt caacctcaac caggtgctga gcaaggtg
//
LOCUS       DASZZC010000301         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1118626,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000301 DASZZC010000000
VERSION     DASZZC010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118626"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VHE37_06285"
     CDS             <1..605
                     /locus_tag="VHE37_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT35169.1"
                     /translation="LAPDGNAFRGELGLRDGDIVAMYAGAVGPKQGLRILLDVAPRLP
                     HVQFVVVGAGDEWLALKQMAEQGRLSNLRFAQTQPEDRLPEVINAADIHLILQLGSIT
                     NVVMPSKLANIYACARPVIATAAADTALGRVIEQSGGGILCPPDDAAALAQAVARLAD
                     DADRRSSLGRSGRNYAQSQFAMDAILPQLELRLAAVVSAR"
     gene            607..1284
                     /locus_tag="VHE37_06290"
     CDS             607..1284
                     /locus_tag="VHE37_06290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT35170.1"
                     /translation="MAARLPLRLSLLLKPRGRRRFIQLLPVGASVLDVGCGNGSPQLT
                     LRQRPDLHYTGVDVCDAPPFALPPGAVLQTLEPGQFATGIASLRGRFDAVISAHNLEH
                     CDDPDATLSAMIASLKPGGRLFLAFPCEASIGFPRRAGTLNFHDDPTHRACPSLKQVM
                     AQLEPHCRIEFVSARYRPWLLAMAGLLLEPWSALTRRNAPAGTTWALYGFESVIWAQR
                     RPKAGAP"
     gene            1281..2534
                     /locus_tag="VHE37_06295"
     CDS             1281..2534
                     /locus_tag="VHE37_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35171.1"
                     /translation="MIQRLPMPLAYFGLKSGYVIWRALALLIVAPHTADETYADLALA
                     LLFAETTRYLADWGSEQVSVKRVLEQPAAAQDLWSLKLLLAILHAAANCLMLVLLFPR
                     QSSSVLAGIAVLAALPSLGNFLVSTCYARNELSAAILPMTLAMLLAASGILAASHTWP
                     LQAVVPLMLLAELAQLGVAMAATLPRLQISLARMSWPKQATQVLRAGTPLALAQLLAL
                     IYVRADVIVLRVLAPAVEQARYVFALRSVDVAVFFSGGVGSWLYIVFCQLQARGWPES
                     ERLRLQRNLRRLWLAAIAAVVVVWECALPAFIEARYPQFFASVPLIRVMGCTVLAVLG
                     NQTCTALIQSGHRAVWITCINAASLALIYASLVPAYRGAGPLGIALAVLAIYTLSALV
                     QTMLARRLLRSAVHAAGTTLAPEST"
     gene            2531..3559
                     /locus_tag="VHE37_06300"
     CDS             2531..3559
                     /locus_tag="VHE37_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVT35172.1"
                     /translation="MKEELACLIVARNLGDAVIHSMFFAELVRRGYAQRYLVWARPQA
                     AFLFAGIGDCELVTSPFPAGTNMQMGGLGSFRFLRAAAMVRGKRPSVTIDLIGDFRER
                     WFASLAGSRRHRHIGWAEDHPFHRLIRNPFGTTEPVYRVPATELNVYSAYARMLDRLA
                     PGPQPASLAVQRRAAPTQAPHIGLHPFASQRCREWPLERWRELGAALTQRGCRATIFG
                     APPDAGRLHQLVSGLPVNVHTGSTAQFAAAVAQLDGMVALDSFALHLAARQGVPAVMI
                     NGANDHRIWQPPSALVLAESGGCPVYPCYNKPSCVGGPGEYACIRAIGVGQVLTAVEQ
                     ILGMPQLT"
     gene            3556..>3838
                     /locus_tag="VHE37_06305"
     CDS             3556..>3838
                     /locus_tag="VHE37_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35173.1"
                     /translation="MTSTPATAARPRALLSLLLALAAAWGFWASIRAWLGAPILDLGS
                     LNSPTPRLMAAWDNFAIFGSLCAAAVAWHLHARMETRDWDAWYRMRRQPL"
BASE COUNT          558 a         1373 c         1217 g          690 t
ORIGIN      
        1 cgctcgcgcc cgatggcaac gccttccgcg gcgaactggg gctgcgggat ggagatatcg
       61 tcgcgatgta tgcaggcgcc gtcggcccca aacagggctt gcgcatcctg ctcgacgtgg
      121 caccgcgcct gccgcatgtc cagttcgtcg tcgtcggcgc aggcgacgaa tggcttgcgc
      181 tgaagcagat ggccgagcag ggtcgattgt ccaatctccg cttcgcgcag acgcagccgg
      241 aggaccggct gccggaagtc atcaacgccg ccgacatcca cctgatcctg cagctcggct
      301 cgatcaccaa tgtggtcatg ccgtcgaagc tggccaacat ctatgcctgc gcacgcccgg
      361 tgatcgcgac ggctgccgcc gatacggcgc tgggacgggt gatcgagcaa agcggtggcg
      421 gcatcctgtg cccacccgac gacgcggccg ccctggcgca ggccgtcgca cggctggcgg
      481 atgacgccga ccgccgcagt tccctgggcc gcagtggccg caactacgct cagtcgcaat
      541 tcgccatgga tgcgatcctg ccccaactcg aactgcggct cgccgccgtc gtaagcgcgc
      601 gttgacatgg ccgccagact tcctctgcgt cttagcctcc tgctgaagcc acgcggacgc
      661 cgccgcttca tccagctgct gcccgtcggc gcttcggtgc tcgacgtcgg ctgcggcaac
      721 gggtctccgc agctgacctt gcgccagcgc cccgacctcc actacaccgg cgtcgacgtc
      781 tgcgatgcgc ctccattcgc actgccgcca ggtgcggtct tgcagacgct ggagcccggc
      841 cagttcgcca ccggcattgc gagtttgcgc ggacgcttcg acgccgtgat cagtgcccac
      901 aacctcgagc attgcgacga tcccgatgcc accctgtctg ccatgatcgc gtccctgaaa
      961 ccaggtggcc gcctgttcct ggcatttccg tgcgaggcca gcatcggctt tccgcggcgg
     1021 gctggcacgc tcaacttcca cgacgatccc acgcaccgcg cttgccccag cctcaagcag
     1081 gtaatggcgc agctggagcc acattgccgt atcgaattcg tctccgcccg ctaccggccg
     1141 tggctgctgg cgatggccgg cctgctgctg gaaccctgga gcgcgctgac ccgacgcaac
     1201 gcgcccgccg gtacgacctg ggccttgtac ggattcgaaa gcgtgatctg ggcgcagcgc
     1261 cgacccaaag caggcgcgcc atgatccagc gcctgcccat gccgctcgcc tatttcggcc
     1321 tgaagtccgg ctacgtcatc tggcgtgcgc tggcattgct catcgtggcg ccgcataccg
     1381 ccgacgagac ctacgccgac ctggcattgg cgctgctgtt tgccgaaacc acgcgctacc
     1441 tcgccgactg gggttctgag caggtgtcag tcaagcgcgt gctggagcaa cccgcagcgg
     1501 cgcaggacct gtggagcctc aagttgctgc tggcgatcct gcacgcggca gccaattgcc
     1561 tgatgcttgt gctcctgttc cccaggcaat cgtcatccgt cctcgccggc atcgccgtgt
     1621 tggccgccct gccctcgctc ggcaatttcc tggtaagcac gtgttatgcg cgtaacgaac
     1681 tgagcgcagc catcctgccc atgacgctgg ccatgctgct cgcagcgagc ggaatcctgg
     1741 ccgccagcca tacatggccg ctgcaagccg tggtaccgct gatgctgctg gcggaactgg
     1801 cacagctggg cgtggccatg gccgcgacct tgccgcgcct gcagatctcg ttggcacgca
     1861 tgagctggcc caagcaagcc acgcaggtgt tgcgagccgg gactcccttg gcgctggcgc
     1921 agctgctggc actgatctac gtgcgtgccg acgtcatcgt gctgcgggtt ctggctccgg
     1981 ccgtggagca ggcccgctac gtcttcgcgc tgcgctcggt cgacgtggcg gtgttctttt
     2041 ccggtggtgt cggctcgtgg ctttacatcg ttttctgcca gttgcaagcc aggggctggc
     2101 ccgagtccga gcgtttgcgc ctgcagcgca acctgcgccg cctgtggctg gccgccatcg
     2161 cggccgtggt cgtggtctgg gaatgcgccc tgcccgcgtt catcgaggcg cgctacccgc
     2221 agttttttgc cagtgttccg ctgatccggg tgatgggttg caccgtgctg gccgtgctgg
     2281 gcaaccagac ctgtaccgcg ttgatccagt ccggccatcg cgcggtttgg atcacctgca
     2341 tcaacgcggc cagtctcgcg ctgatctatg ccagcctggt gccggcttat cgcggcgccg
     2401 gtccgctggg catcgcgctc gcggtgctcg ccatctacac actttccgcg ctggtgcaga
     2461 ccatgctggc acgccggctg ttgcgaagtg cagtacacgc cgcaggcacg acattggcgc
     2521 cggaatcgac gtgaaggaag aactggcctg cctgatcgtc gcgcgcaacc tcggcgacgc
     2581 cgtcatccac agcatgttct tcgcagaact ggtgcggcgc ggctatgcgc agcgctacct
     2641 ggtgtgggcg cgaccgcagg cggcgttcct gtttgccggc atcggcgatt gcgaactggt
     2701 cacgtcgcca ttccctgcag gcaccaacat gcagatgggc ggcctcggca gcttccgctt
     2761 cctgcgggca gcggccatgg tccgcggcaa acgccccagc gtcaccatcg atctgatcgg
     2821 cgacttccgc gaacgctggt ttgcctcgct cgccggcagc cgtcggcatc ggcacatcgg
     2881 ctgggctgaa gatcatccgt tccaccggct gatccgcaat cctttcggca cgaccgagcc
     2941 ggtctatcgg gtgccggcga cggaactgaa tgtttactcg gcctatgcgc gcatgctcga
     3001 ccgcctggcg cccgggccgc agccggcgtc gctggcggtg cagcgccgcg ccgcgcccac
     3061 gcaggcgccg cacatcggcc tgcatccatt cgcctcgcag cgctgccgcg agtggccact
     3121 ggaacgctgg cgcgaactcg gcgccgcact cacccagcgc ggatgccgcg caaccatttt
     3181 cggcgcgccg cccgacgccg ggcgactcca ccaattggtc tcaggcctgc ccgtcaacgt
     3241 gcacaccggc agcaccgcgc aattcgccgc cgccgtcgcg caactcgacg gcatggtcgc
     3301 gctggacagc ttcgccctgc acctcgccgc caggcagggc gtaccggcgg tgatgatcaa
     3361 tggcgcgaac gaccatcgca tctggcaacc gccgtcggcg ctggtgctgg ccgaatcggg
     3421 cggttgcccg gtctacccct gctacaacaa gcccagctgc gttggcggcc cgggcgaata
     3481 tgcctgcatc cgcgcgattg gcgtcggaca ggtgctgacc gccgtcgaac aaatccttgg
     3541 aatgccgcaa ctcacatgac ttccacgccc gctactgctg cccgaccacg cgcattgctc
     3601 agcctgctgc tggcgctggc cgccgcctgg ggtttctggg catcgatccg cgcctggctc
     3661 ggcgctccga tcctcgatct gggttcgctg aacagcccca caccgcggct gatggcagcg
     3721 tgggacaact tcgccatctt cggcagcctg tgcgcggccg cagtggcctg gcacctgcac
     3781 gcccgcatgg agacgcgcga ctgggatgcc tggtaccgca tgcggcgcca gccgctgc
//
LOCUS       DASZZC010000302         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_27085,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000302 DASZZC010000000
VERSION     DASZZC010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27085"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2610)
                     /locus_tag="VHE37_06310"
     CDS             complement(67..2610)
                     /locus_tag="VHE37_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVT35174.1"
                     /translation="MTAQTVSTGFTRRILKIVEVPIFHWRRGTLALLIALTCLFGWNL
                     AQLKPDAGWLKMVPKSHPYMATFMQYYRDFGGANTVLIAVHNEHGDIYQPQFMETLRQ
                     VADEAFYIPGADRARVVSIFSPSILYIEVVEGGLSGENVIPSNYAPTPEMMERIRTNV
                     AKASVIGRLVSEDQTTALVQLELLEHDPTAGTTADLATAGRSLPERLRAAWAASAGSS
                     LVERLRALQAASSGNRPAALDYVKVGDKLEEIRRRYEHDGVTIHIVGFAKVVDDMTKA
                     SFQVGALFIVALLLMGLLLWLYVGSLRLAQLVVATSLVACTWELGLLHLIGYGLDPFA
                     MLVPFLIMAVSVSHGVQYVSAWANEISTRGATPYQASLATFRSLAIPGVVALLTNVAG
                     FSTIYLIDIQIIREMSINAAFGMLGVIVVNKVLLPIVLSYLRLPDPAAFRAAQEKRER
                     IGDAVFRRMALLTRRGPATVVVIIMLLLAAFGASQYRHVQIGDTTAGVPELRPTSRFN
                     QDAAVIARHFTMGVDHLKVIAESFDDGCVDYGVMSEIDRFSWYMQNQASVRDVLSLPE
                     LAKIAHAGLNEGRLDAEVIPRQPQTLAQITALVPTTTGFLNSTCSALALFIFTTDHKA
                     QTISALTDAVQRYNATLQQDHRVKFRLASGNIGVMAATNEEVRRNELPVVAWVYGVII
                     LFLWLSFRTLSGVLCVILPLSTVTLLGYAVMVGLGIGQKVATLPVLAFACGIGVDYGI
                     YSYSVVAQGLRRGLSLEEAYYQKMRSTGKATLFTGVGLATGVAMWLFSGLQFQRDMGV
                     LLIFGFTANMIGAIVVLPALAHFLSGEERRHADKDLSAGADDALKEQER"
     gene            complement(2607..>2804)
                     /locus_tag="VHE37_06315"
     CDS             complement(2607..>2804)
                     /locus_tag="VHE37_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35175.1"
                     /translation="ALSLSPASALLVGFNGAAQKLDLAAGTLAPVMVPASETLVKLVD
                     FHGHLIAVGRRGAQDLGPVTK"
BASE COUNT          491 a          935 c          948 g          430 t
ORIGIN      
        1 gcgcggtggc gcggccgatg ccgctggccg cgccggtgac caggcagcgc gcgccctgca
       61 ggcggctcat cgctcctgtt ccttcagcgc gtcgtcggcg ccggcggaaa gatccttgtc
      121 ggcatggcgc cgctcttcgc cggagaggaa atgcgccagc gccggcagca ccacgatggc
      181 gccgatcatg ttggcggtga agccgaagat cagcagcacg cccatgtcgc gctggaactg
      241 caggccggag aacagccaca tggcgacgcc ggtggcgagg cccacgccgg tgaacagcgt
      301 tgccttcccg gtcgagcgca tcttctggta gtaggcctct tccagcgaca gcccgcggcg
      361 caggccctgc gccaccaccg aatagctgta gatgccgtaa tccacgccga tgccgcaggc
      421 gaaggccagc accggcagcg tcgccacttt ctggccgatg cccaggccca ccatcaccgc
      481 gtagccgagc agcgtcaccg tcgacagcgg caggatcacg cacagcacgc cggagagcgt
      541 gcggaacgac agccacagga acaggatgat cacgccgtag acccaggcca ccaccggcag
      601 ttcgttgcgg cgcacttcct cgttggtcgc cgccatcacg ccgatgttgc cggaagccaa
      661 tctgaacttg acgcgatgat cctgctgcag cgtggcgttg tagcgctgta cggcgtcggt
      721 cagcgcgctg atggtctgcg ccttgtgatc ggtggtgaag atgaacagcg ccagcgccga
      781 acaggtggaa ttgaggaatc cagtcgtggt cggcaccagc gcggtgattt gcgccagcgt
      841 ctgcggctgg cgcgggatca cctcggcgtc gagccggccc tcgttgaggc cggcatgcgc
      901 gatcttggcc agctccggca gcgacaacac gtcgcgcacg ctggcctggt tctgcatgta
      961 ccagctgaag cggtcgatct ccgacatcac gccgtaatcg acgcagccgt cgtcgaagga
     1021 ctcggcgatg accttgagat gatccacgcc catggtgaag tggcgcgcga tgaccgccgc
     1081 gtcctggttg aagcgcgatg tcgggcgcag ctccggcacg ccggcggtgg tgtcgccgat
     1141 ctgcacgtgg cggtactggc tggcaccgaa cgcggccagc agcagcatga tgatgaccac
     1201 gacggtggcc ggcccgcggc gcgtgagcag cgccatgcgg cggaacaccg cgtcgccgat
     1261 gcgctcgcgt ttttcctgcg ccgcgcggaa cgccgcggga tcgggcaggc gcaggtatga
     1321 cagcactatc ggcagcagca ccttgttgac cacgatcacg ccgagcatgc cgaaggcggc
     1381 gttgatcgac atttcgcgga tgatctggat atcgatgaga tagatggtgg agaagccggc
     1441 gacgttggtc agcagcgcca ccacgccagg gatggccagc gagcggaagg tggccagcga
     1501 ggcctggtac ggcgtggcgc cgcgcgtgga gatttcgttg gcccaggcgc tcacgtactg
     1561 cacgccgtgg ctcaccgaca ccgccatgat caggaacggc accagcatgg cgaagggatc
     1621 gagcccgtag ccgatcaggt gcagcaggcc cagctcccag gtgcaggcaa ccagtgacgt
     1681 ggcgaccacc agctgcgcca gccgcagcga accgacgtag agccacagca gcaggcccat
     1741 cagcagcagc gcgacgatga acagcgcgcc cacctggaac gacgccttgg tcatgtcgtc
     1801 gaccaccttg gcgaagccga cgatatgaat ggtcacgccg tcatgctcgt agcgccggcg
     1861 gatttcctcg agcttgtcgc ccaccttcac gtagtccagc gcggcgggac gattgcccga
     1921 cgaggctgcc tgcagcgcgc gcagccgctc gaccaatgac gaacccgcgc tcgcggccca
     1981 cgccgcgcgc aggcgctcgg gcaggctgcg gccggcggtc gccaggtcgg cggtggtgcc
     2041 cgcagtcgga tcgtgttcga gcagttccag ctgcaccagc gcggtggtct gatcctccga
     2101 caccaggcgc ccgatcacgc tggccttggc cacgttggtg cggatgcgct ccatcatctc
     2161 cggcgtcggc gcgtagttgg acgggatcac gttctcgccg gacaacccgc cctcgacgac
     2221 ttctatgtag agaatgctcg gcgagaagat cgacaccacg cgcgcgcggt cggcgccggg
     2281 aatgtagaac gcctcgtcgg ccacctggcg cagcgtctcc atgaactgcg gctggtagat
     2341 gtcgccatgc tcgttgtgca cggcgatcag cacggtgttg gcgccgccga agtcgcggta
     2401 gtactgcatg aaagtcgcca tgtacggatg cgacttcggc accatcttca gccagccggc
     2461 gtccggcttg agctgcgcca ggttccagcc gaacaggcag gtgagcgcga tgagcagcgc
     2521 cagcgtgccg cggcgccaat ggaagatcgg cacctcgacg atcttgagga tgcgccgggt
     2581 gaagccggtc gatacggtct gcgcggtcat ttcgtcaccg gccccaggtc ctgtgcgccg
     2641 cggcgcccga cggcgatcag gtgcccatgg aaatccacca gcttcaccag cgtctcgctt
     2701 gccggcacca tgactggcgc cagcgtgccg gccgccaggt cgagtttctg cgcagcgccg
     2761 ttgaagccaa ccagcagcgc gctcgccggt gacagcgaca acgc
//
LOCUS       DASZZC010000303         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1118865,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000303 DASZZC010000000
VERSION     DASZZC010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118865"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1624
                     /locus_tag="VHE37_06320"
     CDS             <1..>1624
                     /locus_tag="VHE37_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35176.1"
                     /translation="GTLDARGVHGADGGDILIHADRDIDVAPAAVLGFGGHSGGRAAL
                     IAERDLDIGKGSVIDGSARSLKGHGGAAELSGHGRLLLGSMPTLGRGGSLLLDPNTVF
                     IYGTNASDCGAANLCISASQVDQSLDNGQETLIVAGNHIQVGSDASIFSQSNSGLLLG
                     IGQTSAGSTGAFGDGQTFPSSGGLSFIRGAATGGSGSIDFLTSLTDGSIASVGIGGAF
                     EAYSALDKGHVTLGDVTAVGGLTVAAVGNIDAGNLFVSGGGFALGSQQVKPSDVVASA
                     SLVSDTGTVKAASASVDALANSYSYTDGNGYAHDVSRGDASLVMRGTSVAVGDTSNFG
                     GVSVFAQGVASADISAHAGGSVSIAGTTFADVSGNATDTATLGGFRSTLSSGLASISL
                     HTDQPTAGNDTGSIVTGGIEAPGPNASINLESGTVATDFIYAQAYLSSFTEGPQSQRY
                     TAKQNGKTILDSDFGVATLSVIGRGGNATQPSVDIGGTTTLLGPAVEATVQAAGDVNF
                     GDSVSITGQGFSISGSGNTLLAALQDGSIAQFF"
BASE COUNT          269 a          591 c          537 g          227 t
ORIGIN      
        1 cggcacgctc gacgcgcgcg gcgtgcatgg cgccgatggc ggcgacatcc tgatccacgc
       61 cgatcgcgac atcgacgtgg ctccggccgc ggtgctcggg tttggcggcc acagcggcgg
      121 ccgcgcggcg ctcattgccg agcgcgatct cgacatcggc aagggcagcg tgatcgacgg
      181 cagcgcgcgc tcgctcaagg gccacggcgg cgctgcggaa ctgtccggtc atggcaggct
      241 gctgctcggc agcatgccga cactgggccg cggtggcagc ctgctgctcg acccgaacac
      301 cgtgttcatc tacggcacca acgcctcgga ttgcggcgcc gccaacctgt gcatcagcgc
      361 gtcgcaggtc gaccagtcgc tcgacaacgg ccaggagacg ctgatcgtcg ccggcaacca
      421 tatccaggtc ggcagcgacg ccagcatctt cagccagagc aattccggcc tgctgctggg
      481 catcggccag accagcgccg gcagcaccgg cgccttcggc gacggccaga ccttcccctc
      541 gtccggtgga ctcagcttca tccgcggcgc cgccaccggc ggcagcggat cgatcgattt
      601 cctgaccagt ctcaccgatg gctcgatcgc cagcgtcggc atcggcggcg ccttcgaagc
      661 gtattccgcg ctggacaagg gccacgtcac gctgggtgac gtcaccgcgg tgggcgggct
      721 caccgtggcg gcagtcggca acatcgatgc gggcaacctg ttcgtcagcg gcggcggctt
      781 tgcgctgggc agccagcagg tgaagcccag cgatgtcgtc gcctccgcct ccctggtcag
      841 cgacaccggc acggtgaagg cggccagcgc cagcgtcgac gccctcgcca acagttacag
      901 ctacaccgac ggcaacggct acgcgcacga tgtcagccgc ggcgatgcca gcctggttat
      961 gcgcggcacc tcggtcgccg tgggcgacac cagcaatttc ggcggcgtca gcgtgttcgc
     1021 ccagggcgtg gccagcgccg acatctccgc ccacgcgggc ggcagcgtca gcattgccgg
     1081 caccaccttc gccgacgtga gcggcaacgc cacggacacg gccacgctgg gcggcttccg
     1141 aagcacgctg agctccggcc tggccagcat ctcgctgcac accgaccagc ccaccgccgg
     1201 caacgacacc ggcagcatcg tcacaggcgg catcgaagcg ccggggccca atgccagcat
     1261 caacttggag tccggcaccg tcgcgacgga tttcatctac gcgcaggctt acctgtcgtc
     1321 gttcaccgaa gggccgcagt cgcagcgcta tacggcaaag cagaacggca agaccatcct
     1381 tgacagcgac ttcggcgtgg ccacgctgtc ggtgatcggc cgcggcggca acgcgacgca
     1441 gcccagcgtg gacatcggcg gcaccaccac cctgctcgga cccgcagtgg aggccacggt
     1501 gcaggcggcg ggcgacgtca acttcggcga cagcgtcagc atcaccggcc agggcttcag
     1561 catcagcggc agcggcaaca cgctgcttgc cgccttgcag gacggctcga tcgcgcagtt
     1621 cttc
//
LOCUS       DASZZC010000304         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_27283,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000304 DASZZC010000000
VERSION     DASZZC010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27283"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="VHE37_06325"
     CDS             complement(<1..1865)
                     /locus_tag="VHE37_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HVT35177.1"
                     /translation="MFFKSKSHATDELAGPGWLERLPREAAGLACAGCSLFLLVTLVS
                     FSPNDHAWSSSGNGGAVSNLAGSLGAWIADLLLSAVGYVAFLLPWAVLMVGVRLLRPP
                     ADVQPLPAPMRWASWMLVISSLAALCSLHVGAAPALAPQGAGGIAGLVLAGALVSVLK
                     PFGATLILLTFFAACAPLALTFSWLQLFEDIGGWTLRMVEQRRAAAQPVDEELPQAAE
                     LADLEAAEEEVKPREKKKRRLLPSLTGSEEEQPDLFQPPPELPERRTRLVKKTERGSV
                     PAFDGDPLPPIDILDAPRPSGKRFTPEEIERLSRDVEKHLDDFGVEAQVVAATPGPVI
                     TRFELQPAPGVKGSQITNLSKDLARALSVASVRVIEVIEGKSVIGLEIPNQKREMVVL
                     REIFEAPVYKNATSPLTLGVGKDIAGNPVAVDLGKMPHLLVAGTTGSGKSVAINGMIL
                     SMLYKATKDQVRFIFIDPKMLELSVYEGIPHLLAPVVTDMKDAANALRWCVGEMERRY
                     KLMSALGVRNLAGLNKKLEDAAKNGEPIRDPLAKPPDLFDEEKPPFEPEELKPLPNIV
                     VIIDELADMMMVVGKKVEELIARLAQKARAAGIHLIVATQRPSVDVITGLIKANIP"
BASE COUNT          313 a          659 c          598 g          311 t
ORIGIN      
        1 gggatattcg ccttgatcag gccggtgatg acgtccacgc tcggacgctg ggtggcgacg
       61 atcaggtgga tgccggccgc gcgcgccttc tgtgccagac gcgcgatcag ctcttcgacc
      121 ttcttgccga ccaccatcat catgtcggcc agttcgtcga tgatcaccac gatgttcggc
      181 agcggcttca actcctccgg ctcgaacggc ggcttctctt catcgaacag gtccggcggc
      241 ttggccagcg gatcgcggat cggctcgcca tttttcgccg cgtcttccag cttcttgttg
      301 aggccggcga gattgcgcac gcccagcgcc gacatcagct tgtagcggcg ttccatctcg
      361 cccacgcacc agcgcagcgc gttggcggcg tccttcatgt cggtgaccac cggcgccagc
      421 aggtgcggga tgccttcgta gacggaaagc tccagcatct tcgggtcgat gaagatgaag
      481 cgcacctggt ccttggtcgc cttgtacagc atcgacagga tcatgccgtt gatcgccacc
      541 gacttgcccg agccggtggt gccggccacc agcaggtgcg gcatcttgcc caggtcgacc
      601 gcgaccggat tgccggcgat gtccttgccc acgcccagcg tgagcggcga ggtggcgttc
      661 ttgtacaccg gcgcttcgaa gatctcgcgc agcaccacca tctcgcgctt ctggttgggg
      721 atctccaggc cgatgaccga tttgccttca atgacttcta tgacgcgcac gctggccacc
      781 gacagcgcac gcgccagatc cttcgacagg ttggtgatct gcgagccctt cacgcccggc
      841 gccggctgca gttcgaagcg cgtgatcacc gggcccggcg tggccgccac cacctgcgct
      901 tccacgccga aatcatccag gtgcttttcc acgtcgcgcg acaggcgctc gatctcttcc
      961 ggcgtgaagc gcttgcccga cgggcgcggc gcatcgagga tgtcgatcgg cggcagcggg
     1021 tcgccgtcga acgccggcac gctgccgcgc tcggtcttct tcaccaggcg cgtgcggcgc
     1081 tcgggcaatt ccggcggcgg ctggaacagg tcgggctgtt cttcctcgct gccggtcagc
     1141 gatggcagca gccggcgctt cttcttctcg cgcggcttga cctcttcctc ggcggcttca
     1201 aggtcggcca gctctgcggc ttgcggcaat tcttcgtcca ccggctgcgc ggcagcgcgg
     1261 cgctgctcca ccatgcgcag tgtccagccg ccgatgtcct cgaacagctg gagccacgaa
     1321 aaggtcagtg ccagcggcgc gcacgcggcg aagaaggtga gcaggatcag cgtggcgccg
     1381 aacggcttga gcaccgacac cagcgcaccg gccagcacca gcccggcaat gccgccggca
     1441 ccctgcggcg ccagcgccgg cgcagcaccg acatgcagcg aacagagagc cgcgaggctg
     1501 ctgatcacca gcatccacga ggcccagcgc atcggcgcgg gcagcggctg cacatcggcc
     1561 ggcggacgca gcaggcgcac gcccaccatc agcaccgccc acggcagcag gaaggcaacg
     1621 tagccgaccg cggacagcag caggtcggca atccaggcgc cgaggctgcc ggcgagattg
     1681 ctcaccgcgc cgccgttgcc cgaagacgac cacgcatggt cgtttggcga aaaactgacc
     1741 agcgtcacca gcaggaacag cgagcagccg gcgcaagcca ggcccgcggc ttcgcgcggc
     1801 aggcgctcca gccatcccgg cccggccagc tcatccgtgg catgagattt ggatttgaaa
     1861 aacatagcac ctatacgcaa t
//
LOCUS       DASZZC010000305         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1244011,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000305 DASZZC010000000
VERSION     DASZZC010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244011"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHE37_06330"
     CDS             complement(<1..252)
                     /locus_tag="VHE37_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT35178.1"
                     /translation="MPRKPASQQIDTSRALQDCAFEMFGRCGYEGVSIGDIAATTKLS
                     KGALYWHFRGKEELYLACQARLHALFNDYIFDPMRAEPDA"
     gene            300..1079
                     /locus_tag="VHE37_06335"
     CDS             300..1079
                     /locus_tag="VHE37_06335"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HVT35179.1"
                     /translation="MPLIGDVIRAAAMDLAGARRVLAITGAGVSADSGLPTYRGIGGL
                     YDDAATEDGYAIEQALSGHMMRVRPEVCWKYIHQIESSCRGARHNAAHEALAQMEQRF
                     EHFTLLTQNIDGFHRAAGSRNLIEIHGNVQQLYCTACADERSVNSYAGLAIPPACVRC
                     GALVRPRVVLFGERLPEGELGRLYAALYRGVDAVLTIGTTSVFPYIAEPVVRAARAGV
                     PTIEINPGETEVSRFVRHRIRERAAVVLPQIMALLAAASRS"
     gene            complement(1045..2133)
                     /locus_tag="VHE37_06340"
     CDS             complement(1045..2133)
                     /locus_tag="VHE37_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35180.1"
                     /translation="MNAPDGLTEHGFRRVDEFSSARNSWPRGTQPALARAAAEKFRAR
                     FKSGPQVQGVRSVDLVSAGYPAGFAFHNAAFYLNPFLAICNRIVLVRFTDFLGRPKLL
                     AWEPTIPQNSEKAPFFAFLVRAFGDFLSHRVFMTEFNTIPQALARCGVKAEEVDYFAF
                     DHLHVQEVRGLLGTKDGSIKPVFPNAKMIVQPRELDTVMSPHPMQHAWYVPDGGAGID
                     LSRVIQIEGDFELGAGIALIATPGHTDGNMTLALNTPDGIWVTSENGVAIDNWFPEHS
                     RIPGIKTYMRHFGREVVLNSNTLEDPQDQYNSMLIEKTLADVSKKDPRYKQVLPSTEL
                     LPWLRNWPCTPGLFQGGISYGTLQPAAP"
     gene            complement(2130..>2313)
                     /locus_tag="VHE37_06345"
     CDS             complement(2130..>2313)
                     /locus_tag="VHE37_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35181.1"
                     /translation="AAHAGVRASFFDHLPLGFGRMARGYLAARRFGVGALAKPLLSAL
                     SNVEILVLEKPAGKSA"
BASE COUNT          376 a          757 c          770 g          410 t
ORIGIN      
        1 tgcgtcgggt tccgcgcgca tcgggtcgaa gatgtagtcg ttgaacagcg cgtgcagccg
       61 cgcctggcag gccaggtaca gctcttcctt gccgcggaaa tgccagtaga gcgctccctt
      121 cgacagcttg gtggtagcgg cgatgtcgcc gatcgatacg ccttcgtagc cgcagcgtcc
      181 gaacatctcg aaggcgcaat cctgtagcgc gcggctggtg tcgatctgct ggctggcggg
      241 tttgcgtggc atggctggca ttatgccgac gcgtccgaat gtatggttaa catggcgcca
      301 tgcccctcat cggcgacgtc atccgcgctg ctgcaatgga ccttgccggc gcgcggcgcg
      361 tgctggcgat caccggcgcc ggcgtttccg ccgactccgg cctgcccacc tatcgcggca
      421 tcggcggcct ctatgacgat gcggcgaccg aagatggcta cgccatcgag caagccctgt
      481 ccggccacat gatgcgcgtg cgtcccgagg tgtgctggaa atacatccac cagatcgagt
      541 catcctgccg cggcgcgcgc cataacgccg cgcacgaagc gctggcgcaa atggagcagc
      601 gctttgagca tttcacgctg ctgacgcaga acatcgacgg tttccatcga gcggccggca
      661 gccgcaacct catcgagatc cacggcaatg tgcagcagct gtactgcacc gcctgcgccg
      721 acgagcgcag cgtaaacagc tatgccggcc tggccattcc gccggcatgc gtgcgttgcg
      781 gcgcgctggt gcggccgcgc gtggtgttgt tcggcgagag gttgccggaa ggggaactgg
      841 gccggctgta cgccgcgctg taccgcggcg tggacgcggt gctgaccatc ggcaccacca
      901 gcgtcttccc gtacatcgcc gagccggtgg tccgcgcggc acgtgccggc gtgccgacca
      961 tcgagatcaa tccgggggaa accgaagttt cacgcttcgt gcgtcaccgt atccgcgaac
     1021 gcgcggcagt ggtgctgccc cagatcatgg cgctgctggc tgcagcgtcc cgtagctgat
     1081 cccgccctgg aacaatcccg gcgtgcaggg ccagttgcgc agccagggca gcagttcggt
     1141 cgacggcagc acctgcttgt agcgcgggtc tttcttcgac acgtcggcca gggtcttctc
     1201 gatcagcatc gagttgtact ggtcctgcgg gtcttccagc gtgttcgagt tgagcaccac
     1261 ctcgcggccg aaatggcgca tgtaggtctt gatgcccggg atgcgcgagt gttccgggaa
     1321 ccagttgtcg atggccacgc cattttccga ggtcacccag atgccgtccg gcgtgttcag
     1381 cgccagggtc atgttgccgt cggtgtggcc gggcgtggcg atcagcgcga tgccggcgcc
     1441 gagttcgaag tccccttcga tctggatcac gcgtgacagg tcgatgccgg ctccgccgtc
     1501 gggcacgtac caggcatgct gcatcgggtg cggcgacatc accgtgtcca gttccctggg
     1561 ctgcacgatc atcttcgcgt tggggaagac cggcttgatc gagccatcct tggtccccag
     1621 cagcccccgc acttcctgca cgtgcaggtg atcgaacgcg aagtagtcca cctcttccgc
     1681 cttcacgccg cagcgcgcca gcgcctgcgg aatggtgttg aactcggtca tgaacacgcg
     1741 gtgcgagagg aagtcgccga aggcgcgcac caggaaggcg aagaacggcg ccttctccga
     1801 gttctgcgga atggtcggct cccaggccag cagcttcggc cgcccgagaa aatcggtgaa
     1861 gcgcaccagc acgattcggt tgcagatcgc caggaacgga ttcaggtaga acgcggcgtt
     1921 gtggaaggcg aaaccggcgg gatagccggc cgataccagg tccacgctgc gcacgccttg
     1981 cacctgcggg ccggacttga agcgcgcgcg gaatttttcc gccgccgcgc gcgccagggc
     2041 cggttgcgtg ccgcgcggcc agctgttgcg ggcgctgctg aattcatcaa cgcggcggaa
     2101 gccgtgctcg gtcagcccat cgggcgcgtt catgcgcttt tccccgccgg cttctccagc
     2161 accaggatct cgacattgga cagtgcggac aacagcggct ttgccagcgc gccgacgccg
     2221 aacctgcggg ccgcgaggta accgcgcgcc attctgccga aacccagcgg caggtgatcg
     2281 aagaagctcg cccgcacgcc ggcatgggcg gcc
//
LOCUS       DASZZC010000306         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_856248,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000306 DASZZC010000000
VERSION     DASZZC010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856248"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /gene="cpaB"
                     /locus_tag="VHE37_06350"
     CDS             complement(<1..534)
                     /gene="cpaB"
                     /locus_tag="VHE37_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVT35182.1"
                     /translation="MSSKRSSMVYVALALLLGTGSAFLVQRWAEQRMVAAQQHAADEG
                     AQVVVAAREIEFGKRIEPNHLKLVPWPASALPEGAFSNLSDVVGKLSKHDILPGEPVL
                     AARVADPASGALLASLIEPNKRAVAVRVDDVVGVGGFLLPGSRVDVVASRRIEENRYD
                     TRTVLYNLKVLAVDQTAS"
     gene            complement(680..1876)
                     /locus_tag="VHE37_06355"
     CDS             complement(680..1876)
                     /locus_tag="VHE37_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT35183.1"
                     /translation="MRPFMLQRGIILVTFVVSLLVLLLFFGMAVDVGFIYLARASLSK
                     GTDAAALMAVRNISLGDAATTAIAQSTFDMNYQASSLPSRQVARPTVTVGYSLDSMGN
                     KQVTVSATLQLNTFILGVIPDLARAQVSSSAQAARARLIMGIVLDRSGSMTTNGGSAA
                     LPPAVTTFINFFDDANDRASMDSFSDNARLDVAMGHNFKASITSKVKSMAFGGWTYSH
                     GGIDIGRTQINSVALAPGENVVRVLVFFTDGQANSFLANNYPCTKTSSRSLVLVPGNG
                     TSDFNDPATGNSVTCSSSTTKTFFSQKYNANRTRNTNNVTEEGSWMAEHSAQLLRGDG
                     TLVFSIGLGTDIDKASLHRIANDASSTTYNPNQPIGVAAFAPTANDLDDVFRQIAAKI
                     LLRLTL"
     gene            complement(1881..2381)
                     /locus_tag="VHE37_06360"
     CDS             complement(1881..2381)
                     /locus_tag="VHE37_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HVT35184.1"
                     /translation="MNARARGLAVVEFAMVLPFMLLLLFGVVDLGRMVLTRQILINVS
                     REASNLTSRGTLPSDAIAAVQISAQPLNFPVEGFVIISEVARSSAGALTVKSRASGGG
                     RPQPSRIGASVGGSATLPVTSTPIPPNGQSVFVTEVYYHSPPVTPLGSLIGQTIGDIF
                     YDVAFF"
     gene            complement(2378..2869)
                     /locus_tag="VHE37_06365"
     CDS             complement(2378..2869)
                     /locus_tag="VHE37_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HVT35185.1"
                     /translation="MVEQPKVAVAASAVRRRGMVTVEAAIVLPLFMLLMFCIIDFGRL
                     LFTQTTLQHAMREGGRYGVTGRRLPNPADPKSLESRLDSIKQIVQQSAIGVNVKPGDI
                     VISSARGGNGNAGGPGDTLTISLTYDFVFATPLVAQFFNHGSYTFTTSTSFRNEPFPP
                     ETP"
     gene            complement(2945..3118)
                     /locus_tag="VHE37_06370"
     CDS             complement(2945..3118)
                     /locus_tag="VHE37_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35186.1"
                     /translation="MNQTQQVSTRPQQQRGLTTVEYAVAGGLIAATVVAAFVLLGDRV
                     NTIIHAIITSLGG"
     gene            complement(3285..3467)
                     /locus_tag="VHE37_06375"
     CDS             complement(3285..3467)
                     /locus_tag="VHE37_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35187.1"
                     /translation="MNDTQQTAAVHSSPRGQRGLTTVEYAVAGGLIAATVVAAFVLLG
                     GRVNTIIHAIITSLGG"
BASE COUNT          608 a         1181 c         1334 g          629 t
ORIGIN      
        1 gctggcggtc tggtcgaccg ccagcacctt gaggttgtag agcacggtgc gggtgtcgta
       61 gcggttttcc tcgatgcggc gcgaggcgac cacgtcgacg cggctgcccg gcagcaggaa
      121 gccgccgacg ccgaccacgt catcgacgcg caccgccacc gcgcgcttgt tcggttcgat
      181 caggctggcc agcagcgcgc cgctggccgg gtcggccacg cgcgcggcaa gcaccggctc
      241 gccgggcagg atgtcgtgct tggacagctt gcccacgaca tcggacaggt tgctgaaggc
      301 gccttccggc agcgccgacg ccggccatgg caccagcttg aggtgattgg gctcgatgcg
      361 cttgccgaat tcgatctcgc gcgccgccac caccacctgc gcgccttcgt cggccgcgtg
      421 ctgctgggcg gcgaccatgc gctgttcggc ccagcgctgc accagaaagg ccgagcccgt
      481 gcccagcaac agcgcgagcg cgacgtacac catggaactg cgtttgctgc tcacagggtt
      541 tttctcctgg ttatgaagcg cgccggtaag ccggcgcggc aaactgcagc cggcgccatt
      601 tctgccggct gaggcaatgc gtatacctgc gccgccgcgc gggagcgttg ataccggtgc
      661 gctgtggaca aacgccgtgt cacagcgtga gcctcagcag gatcttggcg gcgatctgcc
      721 ggaacacgtc gtccaggtcg ttggcggtgg gcgcaaaggc ggccacgccg atcggctggt
      781 tggggttgta ggtggtgctg gacgcatcgt tggcaatgcg gtgcagcgag gccttgtcga
      841 tgtcggtgcc caggccgatg gagaacacca gcgtgccgtc gccgcgcagc agctgggctg
      901 agtgctcggc catccatgag ccttcctcgg tcacgttgtt ggtgttgcgc gtgcggttgg
      961 cgttgtactt ctgcgagaaa aaggtcttgg tggtgctgga actgcaggtc accgaatttc
     1021 cggtagccgg atcgttgaag tcggaggtgc cgttgcccgg caccagcacc aggctgcggc
     1081 tgctggtctt ggtgcatggg tagttgttgg ccaggaagga gttggcctgg ccgtcggtga
     1141 agaacaccag cacgcgcacc acgttttcgc ccggcgccag ggccacgctg ttgatctgcg
     1201 tgcggccgat gtcgatgccg ccgtgggaat aggtccagcc gccgaaggcc atgctcttca
     1261 ccttgctggt gatgctggcc ttgaagttgt gccccatggc cacgtcgagc cgcgcgttgt
     1321 cggagaagct gtccatcgac gcgcggtcgt tggcgtcatc gaagaaattg atgaaggtgg
     1381 tgacggccgg cggcagcgcg gccgagccgc cgttggtggt catcgagcca gagcggtcga
     1441 gcacgatgcc catgatcagc cgcgcgcgcg cggcctgcgc actggagctg acctgcgcgc
     1501 gcgccaggtc ggggatgacg ccgaggatga aggtgttgag ctgcagcgtg gcgctcaccg
     1561 tcacctgctt gttgcccatg gagtcgagcg aatagcccac cgtgaccgtg ggccgcgcca
     1621 cctggcgtga cggcagcgac gacgcctggt aattcatgtc gaaggtgctc tgcgcgatgg
     1681 cagtggtggc cgcgtcgccc agcgaaatat tgcgcaccgc catcagcgcg gcggcatcgg
     1741 tgcccttgga cagcgaggcg cgcgccaggt agatgaagcc gacgtccacc gccatgccga
     1801 agaacagcag cagcaccagc aggctgacca cgaaggtcac caggatgatg ccgcgctgga
     1861 gcatgaatgg gcgcatggcg ctagaagaag gccacgtcgt agaagatgtc gccgatggtc
     1921 tggccgatca ggctgcccag cggcgtcacc ggcggcgagt ggtaatacac ctcggtgaca
     1981 aacacgctct gcccgttggg cgggatcggc gtgctggtca ccggcagcgt ggcgctgccg
     2041 ccgacactgg cgccgatgcg gctgggctgc gggcgaccgc cgccggaggc gcgcgattta
     2101 accgtgagcg cgccggcgct gctgcgcgcc acctcgctga tgatgacgaa gccttccacc
     2161 gggaaattca gcggctgcgc cgagatctgc accgcggcga tggcatcgct gggcagcgtg
     2221 ccccgcgagg tcaggttgga ggcctcgcgg ctgacgttga tcaggatctg ccgcgtcagc
     2281 accatgcggc cgaggtcgac cacgccgaac agcagcagca gcatgaacgg cagcaccatg
     2341 gcgaactcca ccaccgccag cccgcgggca cgcgcgttca tggcgtctcc ggcgggaagg
     2401 gttcgttgcg gaagctggtg ctggtggtga aggtgtacga gccgtggttg aagaattgcg
     2461 ccaccagcgg ggtggcgaag acgaagtcgt aggtgagcga gatggtcagc gtgtcgccgg
     2521 ggccgccggc attgccgttg ccgccgcgcg cgctggagat gacgatatcg cccggcttca
     2581 cgttcacgcc gatggccgac tgctgcacga tctgcttgat cgaatccagc cgcgactcca
     2641 gcgacttggg atcggccggg ttgggcaggc gccgcccggt gacgccgtag cggccgcctt
     2701 cgcgcatggc atgctgcagc gtggtctggg tgaacagcag gcggccgaaa tcgatgatgc
     2761 agaacatcag cagcatgaac agcggcagca cgatggcggc ttccaccgtc accatgccgc
     2821 ggcggcgcac cgcggacgcg gcaactgcta cttttggctg ctctaccatg tgcctgcccg
     2881 actcaggcgg tagagccggc acgtgctcac gcgcggccgg ctttccgtcc gtgaaggatc
     2941 gcgctcagcc gcccagcgag gtgatgatgg cgtggatgat cgtgttcacg cggtcgccca
     3001 gcagcacgaa ggcggcgacg acggtggcgg cgatcaatcc accggccacg gcatattcca
     3061 ccgtggtcag gccgcgttgc tgctgcggcc gcgtggacac ctgttgcgtt tgattcatgg
     3121 caatgctccg aatgagtggg ggtcgtatcg acgccgccgc gggttgcatc gccggcgccg
     3181 tcaacgcccg tccactttca ccccgattgc gctacgcggc attgacctgg atcagtcggc
     3241 accgcgagcg gcggcgcatc cctgcgccgg cggctgccct gcgctcagcc gccgagcgag
     3301 gtgatgatgg cgtggatgat ggtgttgacg cggccaccga gcagcacgaa ggccgccacc
     3361 acggtcgccg cgatcaggcc gcccgcgacc gcgtattcca cggtggtcag gccgcgctgg
     3421 ccgcgaggcg agctgtgcac cgctgcagtc tgttgggtgt cgttcatttg cagatctccg
     3481 attcaggggt tgggcgctgt ccggcactgc gctgggcaga cgcgccggca cctttgcaat
     3541 cggcagacca gccttgcgct gcccgcgcag gtccgccacc agagcaatat cggcaaaagt
     3601 tgtttgtttg aaactagaca agagcgcgcg tcgcgctggg aataaccgcg cgcaacgacg
     3661 gattcacgtc gccggcgagg gacgcgggcg cgtcaaaaaa atgacgcgcg cggcgcggcg
     3721 gccgcgcggt tcatcgagcg aaggtcttca gg
//
LOCUS       DASZZC010000307         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_278545,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000307 DASZZC010000000
VERSION     DASZZC010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278545"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="VHE37_06380"
     CDS             <1..1880
                     /locus_tag="VHE37_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT35188.1"
                     /translation="WPGLPVVLLSGVHSFEKAAAAMALGAHDLVGQEDQHQLRHLELV
                     VKREFAHSHALRELNQTRTRLTDFESRHKQLLDGTNDAVAHIQEGILSHVNPAFAQML
                     GYEHPEDLLSLPLMDIIGTEHVGKVKEHLKLLNKGKVDDKPLECALKKAGGGNVPISA
                     KLTRGTVDGENFIEMLIRAEAPPAAAAPAPAAPVTMPAAAPAPAATPAQAASGRMAFV
                     NALATGVQGSQKMVPGALFLAVDDYAGVEERLGFRDAEEAVIKLTDWVKQKLTPTDQL
                     FRFSTGELAALVVRATVAEVEQIANLLAKQAPEQVFATQGHEAHLSLTVICYPLSGGE
                     NPVAVVNDIVREARKASKAGGKQHVVMGPTAKSSAAERDEARRATQIKRAIEDNRLKL
                     AYQSIASLEGDTRQHFDVLLRMVDETGKEMHAGEFIGAAEKFGLMRTIDRWVTLRALK
                     ILSKRESGQEASSLFVKISEETLKDAEGFIGWMAEVIKQRPLKPEELVFQFQETRLQS
                     HIGKAKLISKALASQGAAICIEHFGVGTSTPQLLEHVPASFVKFHPSFTQQFNDKETQ
                     RKMTQLMELAKLKNIKTIVSHVEDANVMARLWQMGVNYIQGFHIQEPEVVLLATDLFS
                     R"
     gene            complement(2324..3205)
                     /gene="htpX"
                     /locus_tag="VHE37_06385"
     CDS             complement(2324..3205)
                     /gene="htpX"
                     /locus_tag="VHE37_06385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HVT35189.1"
                     /translation="MKRVFLLIATNFAVMIVLSLICQVLGVDHYLMRRGLNLGALLTM
                     AAIFGFGGAFFSLAISKWMAKMSTGAQVIEQPRNSQEAWLVSTVQRLSRSAGIGMPEV
                     AIFDAPEPNAFATGMTKNSSLVAVSTGLLANMNQDEVEAVLGHEITHISNGDMVTLTL
                     VQGVMNTFVFFLARVIGFVVDSALRRDDRDSGPGFGYMITVFVMQMVLGVLASMIVAW
                     FSRQREFRADAGGAYLAGKRKMIAALERLKVGHDETHLPQQMQAFGISGGVASLFATH
                     PPLDVRIAALQSAEAVR"
     gene            complement(3281..>4420)
                     /locus_tag="VHE37_06390"
     CDS             complement(3281..>4420)
                     /locus_tag="VHE37_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVT35190.1"
                     /translation="NHRLQKVTDRLHLLEGLLIAFLNIDEVIHIIRNNDEPKPVLMRK
                     FALTDTQAEYILELKLRHLAKLEEMKIKGEQKELAAERKKIEELLDDDSKLKKLIGDE
                     LKEDAQKYGDKRRCPINAQPPAVALEATEILPAEPITVVLSKAGWIRAAKGFDIDPAA
                     LSYKAGDEFQASAQGKTNQLLVLMDNRGRSYTLNPRELPSARSQGEPVTTKLDLGDGA
                     RIVSIVLGDAAEKYVLGADDGYGFIAQLGELESRLRAGKAVVNVSENASALAPSRVWN
                     VAQDRYAVATAEGRLLVFPLSELPEMAKGKGNKLVTVRDEDRIVAACVIPAGASLVLV
                     SGKRPFTLKPADIEHYAGSRAHRGNVLPRGYRTVNAMMAEAKTSA"
BASE COUNT          789 a         1479 c         1433 g          719 t
ORIGIN      
        1 tgtggcccgg cctgccggtg gtgctgctgt ccggcgtgca cagcttcgag aaggcggcgg
       61 cggccatggc gctgggcgcg catgacctgg tgggccagga agaccagcac cagctccggc
      121 acctggaact ggtggtcaag cgcgaattcg cccacagcca cgcgctgcgc gagctcaacc
      181 agacgcgcac gcgcctcacc gatttcgagt cgcgccacaa gcagctgctg gacggcacca
      241 atgacgcggt ggcgcatatc caggaaggca tcctgtcgca cgtcaacccg gccttcgcgc
      301 agatgctcgg ctacgagcac ccggaagacc tgctgtcgct gccgctgatg gacatcatcg
      361 gcaccgagca cgtgggcaag gtcaaggaac atctcaagct gctgaacaag ggcaaggtcg
      421 acgacaagcc gctggaatgc gcgcttaaga aggccggcgg cggcaacgtg ccgatcagcg
      481 ccaagctcac ccgcggcacc gtcgacggcg agaacttcat cgagatgctg atccgcgccg
      541 aggcgccgcc cgctgcggca gcacccgccc cggcggcccc ggtcacgatg cccgctgcag
      601 cacccgcgcc ggcggccacg cccgcgcagg ccgcctccgg ccgcatggcg ttcgtcaacg
      661 cgctcgccac cggcgtgcag ggctcgcaga agatggtgcc cggcgcgctg ttcctggcgg
      721 tggatgacta cgccggcgtg gaggagcgcc tgggcttccg cgacgccgag gaagcggtga
      781 tcaagctgac cgactgggtc aagcagaagc tcacgccgac cgaccagctg ttccgcttct
      841 ccaccggcga gctggccgcg ctggtggtgc gcgccactgt ggccgaggtg gagcagatcg
      901 ccaacctgct agccaagcag gcgccggaac aggtgttcgc cacccagggc cacgaggcgc
      961 acctctcgct caccgtgatc tgctacccgc tctccggcgg cgagaacccg gtggccgtgg
     1021 tcaacgacat cgtgcgcgag gcccgcaagg catccaaggc cggcggcaag cagcacgtgg
     1081 tgatggggcc aaccgccaag tcctccgccg ccgagcgcga cgaggcgcgg cgcgccacgc
     1141 agatcaagcg cgccatcgag gacaaccggc tcaagctggc ctaccagtcc atcgccagcc
     1201 tggaaggcga cacgcgccag cacttcgacg tgctgctgcg catggtcgac gagaccggca
     1261 aggaaatgca cgccggcgaa ttcatcggcg ccgccgagaa gttcggcctg atgcgcacca
     1321 tcgaccgctg ggtcacgctg cgcgcgctga agatcctgtc caagcgcgag agcggccagg
     1381 aagcctcctc gctgttcgtc aagatttccg aggagacgct gaaggacgcg gagggcttca
     1441 tcggctggat ggccgaggtg atcaagcagc gcccgctcaa gcccgaggag ctggtgttcc
     1501 agttccagga gacgcgcctg cagagccaca tcggcaaggc caagctgatc agcaaggcgc
     1561 tggccagcca gggcgcggcc atctgcatcg agcatttcgg cgtcggcacc tccacgccgc
     1621 agttgctcga acacgtgccg gccagcttcg tgaagttcca ccccagcttc acgcagcagt
     1681 tcaacgacaa ggaaacgcag cgcaagatga cccagctgat ggagctggcc aagctgaaga
     1741 acatcaagac catcgtcagc cacgtggaag acgccaacgt gatggcgcgg ctgtggcaga
     1801 tgggcgtgaa ctacatccag ggcttccaca tccaggagcc ggaggtggtg ctgctggcca
     1861 ccgacctgtt ctcgcgctga gcctgcgcgc ggcgcaaaaa cgctccaccg cacccgcttc
     1921 tggtcagtta taaaatatgt tatttattta atttattagt attttttata acatccaaga
     1981 tggcggtgcg gtgagatgaa aaaagcggcc cggaggccgc ttttttgttg ctgggaagct
     2041 ctagtcactg cgccgactgc agcgctgcaa tgcgcacgtc caagggcggg tggcgacacg
     2101 agcatacgag cagggacccc gctacgtggg gatgcgagcc atgcgagtgg cgcgcgcatc
     2161 cgcccatcac tccgctcggc ctagcgcacg gcatgggccg attgcaacgc ggcaatgcgc
     2221 acgtccaggc gcgggtggcg acacgagcat acgagcaggg gccccgctac gcggggatgc
     2281 gagccatgcg agtggcgcgc atccgcccat cactccgctc ggcttagcgc acggcctcgg
     2341 ccgattgcaa cgcggcaatg cgcacgtcca ggggcggatg cgtcgcgaac agcgaagcca
     2401 cgccaccgct gatgccgaac gcctgcatct gctgcggcag gtgcgtctcg tcgtggccca
     2461 ccttgagccg ttccagcgcg gcgatcatct tgcgcttgcc ggcaagatag gcgccgccgg
     2521 cgtcggcgcg gaattcacgc tggcgcgaga accacgccac gatcatgctg gccagcacgc
     2581 ccagcaccat ctgcatgacg aacacggtga tcatgtagcc gaaacccggc ccgctgtcgc
     2641 ggtcatcgcg gcgcagcgcg ctgtcgacca cgaagccgat gacgcgcgcg aggaagaaca
     2701 cgaacgtgtt catcacgccc tgcaccagcg tcagcgtgac catgtcgcca ttgctgatat
     2761 gcgtgatctc gtggccgagc accgcctcga cctcgtcctg gttcatgttg gccagcaggc
     2821 cggtggacac ggcgaccagc gaagaattct tggtcatgcc ggtggcgaag gcgttcggct
     2881 cgggcgcgtc gaagatcgcc acttccggca tgccgatgcc ggcactgcgc gacagccgct
     2941 gcaccgtcga caccagccag gcttcctgcg agttgcgcgg ctgttcgatc acctgcgcgc
     3001 cggtcgacat cttggccatc cacttggaga tggccagcga gaaaaacgcg ccgccgaagc
     3061 cgaagatggc ggccatggtc agcagcgcgc ccaggttgag cccgcgccgc atcaggtaat
     3121 ggtcgacgcc cagcacctgg cagatcagcg acagcacgat catgaccgcg aagttggtcg
     3181 cgatcagcag gaacacgcgt ttcatcggca ctcacccctt tcccggaaca gccgggtcat
     3241 ggccattgtg cggcctagga ccgccaattc aagtgatcgt tcaagccgaa gtcttggcct
     3301 cggccatcat cgcgttgacc gtgcggtaac cgcgcggcag cacgttgccg cgatgtgcgc
     3361 gcgagccggc gtagtgctcg atgtcggcgg gcttgagcgt gaacgggcgc ttgcccgaca
     3421 ccagcaccag gctggcgccg gccgggatca cgcaggcggc gacgatgcga tcctcgtcac
     3481 gcacggtgac cagcttgttg cccttgccct tggccatctc cggcagctcc gacagcggga
     3541 acaccagcag gcggccttcc gcggtggcca cggcatagcg gtcctgcgcc acgttccaca
     3601 cgcgcgacgg cgcgagcgcg ctcgcgttct cgctcacgtt gaccaccgcc ttgcccgcgc
     3661 gcaggcgcga ttccagttcg cccagctgcg cgatgaagcc gtaaccgtcg tcggcgccga
     3721 gcacgtattt ctccgccgcg tcgccgagca cgatggaaac gatgcgcgcg ccgtcgccca
     3781 ggtcgagctt ggtcgtcacc ggctcgccct gcgagcgcgc gctgggcagc tcgcgcgggt
     3841 tgagcgtgta gctgcggccg cggttgtcca tcagcaccaa taactgattg gtcttgccct
     3901 gcgcggaagc ctggaattca tcgccggcct tgtacgacag tgcggccggg tcgatgtcga
     3961 aacccttcgc cgcgcggatc cagccggcct tggacagcac cacggtgatc ggctcggccg
     4021 gaagaatctc ggtggcttcc agcgctaccg ccggcggctg cgcgttgatc gggcagcgtc
     4081 gcttgtcgcc gtacttctgc gcgtcttcct tgagttcatc gccgatcagt ttcttcagct
     4141 tgctgtcgtc gtcgagcagt tcctcgatct tcttgcgctc ggccgcgagc tccttctgct
     4201 cacccttgat cttcatctcc tcgagcttgg ccaggtggcg cagcttgagt tccaggatgt
     4261 actcggcctg cgtgtcggtg agcgcgaact tgcgcatcag caccggcttg ggctcgtcgt
     4321 tgttgcggat gatgtggatc acttcatcga tgttgaggaa ggcgatgagc aaaccttcca
     4381 gcaggtgcag gcggtcggtg actttctgca gccgatgatt
//
LOCUS       DASZZC010000308         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_402170,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000308 DASZZC010000000
VERSION     DASZZC010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402170"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VHE37_06395"
     CDS             <1..1332
                     /locus_tag="VHE37_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVT35191.1"
                     /translation="PGIIQYAIAALHPKHLAAITPGGTINRIYADGWYMGGVYYAAFA
                     AHWSQFDQPGASKLFAVQRVQAGDDQCKAAAEKIGPNNLVPSLEAQRYDGDYYQQVSP
                     YNRAPEVTVPTLMVQSWTDPAVGSSALWVFDRLKAKDKRLFVLSGDHGAYHYSTAQDE
                     VMRWMDRWVRGEKNGVEKLPRVRVDFQTTLANAPGFGEIGQASVGWTQSLRDWPPPDT
                     QWRTWHLTADGRLADEAASGKQAGARAYFYPTGTELTADNTQFAVQAQEWAALRYRSE
                     PVKQDTGILGAPQLVFYASSTQADTDFRVTLHDISPEGDVTYLQHGMLRASRRAVDAA
                     RSNAQYVFHPHERDEPLMPGQVYEFRLSLFPLAHVLRAGHALELLVSAPPQAAGFTPL
                     MLPGFNTLLHDAAHDSRLLVPYVPGLKAQAPMPACGALPFQPCRRAALEKK"
     gene            1329..2924
                     /locus_tag="VHE37_06400"
     CDS             1329..2924
                     /locus_tag="VHE37_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HVT35192.1"
                     /translation="MIKRLLVVLVLLVSAGCAPKGVVNACGQLEQLPLDHARVVSAVA
                     KSGGSSYSFVGMFVGVPFFHQPASCRVAIEMTPVDDSLIKAQLWLPENWNGRFQGIGN
                     GGFAGSIDTLSLNFALQRGYAAASTDTGHEASDRDGSWALHHPEKIIDYGYRAIHLTA
                     VNAKKIISAYYGRAPAHAYFGSASNGGREALVEAQRYPEDYDGILAGAPALLPSSTLP
                     TWGWIQQQLHAPGAYISKDKLRAIAAATVRACDAQDGVEDGVIDDPRQCRFDPAVLTC
                     AGEENDQCLTPPQVQALRAIYAGPGAPYHGFEPGGELGDKGGWRDWFTGDKPRAAIEY
                     VYVQEFMRYLVYSDAQWSADQFDLKRDGDAFYRQLHDILDAGDPDLSKFAARGGKLIL
                     YHGWNDGALSPRTTIDYYESVRARMGAKKAQQFITLYMVPGLQHCIGGPGPNAFGQLA
                     PGPVADPRRNISAALEAWVEQGAAPGPIIAGKYDRDITPLLAPEKASLQRTRPLCPYP
                     QVARWSGHGSSDDAANFSCVAPK"
     gene            complement(3022..>4322)
                     /locus_tag="VHE37_06405"
     CDS             complement(3022..>4322)
                     /locus_tag="VHE37_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT35193.1"
                     /translation="ENRSFDHFLGWVPGADGRQAGTTLKDSDGNAHDSFDLAPNFQNC
                     NLADPDHSYSGGRTEINNGQMDGFLLTQPPGDKFPIGYYTADSLPFFKGCAENWTICD
                     RYFSGILSLTTPNRMYMHAGQTDRLANSTTISTLPTIWDRMLGAGRSVKYYYTDVSYT
                     SFWGSKYKSFSQRYEMKSFAADVAAGPLPELMFVDNVGNTMNEGGAISFDDHPYADIR
                     DGQDFLNQVYNVLRAHPDWQRTLMIINYDEWGGFYDHVPPPFAPLTEAEAALGNDGRL
                     GCRVPCVLLGPRAKRGHVEHLQFDPNSILNMLAWRFGFEPLGARASSNNIALALDFDN
                     APDASTPAFDVPSGPFGGLCLPVTLLNGETPITLPLPEPPPIPLPITVQLPSIPGFDV
                     PLTQPDLSFLNTLLQESAARRADHEAELQALRELGAGFGF"
BASE COUNT          730 a         1412 c         1505 g          675 t
ORIGIN      
        1 ccaggcatca tccaatacgc catcgccgcg ctgcatccga agcacctggc cgccatcacg
       61 cccggcggta ccatcaaccg catctacgcc gacggctggt acatgggcgg cgtctactac
      121 gctgcgttcg cggcgcactg gtcgcaattc gaccagccgg gcgcatcgaa actgttcgcg
      181 gtgcagcgcg tccaggcggg tgacgaccag tgcaaggcag ccgcggaaaa aatcggcccc
      241 aacaacctcg tgccgtcgct cgaggcgcag cgctacgacg gcgattacta ccagcaggta
      301 tcgccataca accgcgcgcc ggaagtcacc gtgcccacgc tgatggtgca gagctggacc
      361 gatccggccg tcggcagcag cgcactgtgg gtgttcgacc ggctgaaggc gaaggacaag
      421 cggctgttcg tgctcagcgg cgatcacggc gcgtaccact attccacggc gcaggacgaa
      481 gtgatgcgct ggatggatcg ctgggtcagg ggcgaaaaga acggcgtgga gaagctgccg
      541 cgcgtgcgcg tcgatttcca gaccacgctg gccaatgcac cgggcttcgg cgagatcggc
      601 caggccagcg tgggctggac gcagagcctg cgcgactggc cgccgccgga tacgcagtgg
      661 cgcacctggc acctgaccgc agacggcagg ctggccgacg aagcggccag cggcaagcag
      721 gccggcgcgc gcgcatattt ctaccccacc ggcaccgagc tcaccgccga caacacgcag
      781 ttcgcggtgc aggcgcagga gtgggccgcg ctgcgctacc gcagcgaacc ggtgaagcag
      841 gacaccggaa tactcggcgc gccgcagctg gtgttctacg ccagcagcac gcaggctgac
      901 accgatttcc gcgtgacgct gcacgatatc tcgccggaag gcgacgtcac ctacctgcag
      961 cacggcatgc tgcgcgcttc gcgccgcgcg gtcgacgcgg cgcgctcgaa tgcgcagtac
     1021 gtgttccacc cgcacgagcg cgatgagccg ctgatgccgg ggcaggtgta cgagttcagg
     1081 ctgtcgctgt tcccgctggc gcacgtgctg cgcgcgggcc acgcgctcga gctgctggtc
     1141 agcgcgccgc cgcaggccgc cggcttcacg ccgctgatgc tgcccggctt caacacgctg
     1201 ctgcacgacg cggcgcatga ctcgcgcctg ctggttccgt acgtgccggg cctgaaggca
     1261 caggcgccga tgccggcctg cggcgcactg ccgtttcaac cttgccgcag ggcggcgctg
     1321 gagaagaaat gatcaagcgg cttctggtgg tgcttgtgct gctggtcagc gccggctgcg
     1381 cgcccaaggg cgtggtcaat gcctgcgggc agctcgaaca gctgccgctg gaccacgcgc
     1441 gcgtggtcag cgcggtggcg aaatccggcg gcagcagcta ctccttcgtc ggcatgttcg
     1501 tcggcgtgcc gttcttccat cagccggcgt cgtgccgcgt ggccatcgag atgacgcccg
     1561 tcgacgactc gctgatcaag gcgcagctgt ggctgccgga gaactggaac ggcaggttcc
     1621 agggcatcgg caacggcggc tttgccggca gcatcgacac gctgtcgctc aacttcgccc
     1681 tgcaacgcgg ctacgcggcg gcgtccaccg acaccggcca cgaggccagc gaccgcgacg
     1741 gttcatgggc gctgcatcac ccggaaaaaa tcattgacta cggttatcgc gccatccatc
     1801 tcaccgcggt caacgcgaag aagatcatct cggcgtacta cggccgcgcg ccggcgcatg
     1861 cgtatttcgg cagcgccagc aacggcggcc gtgaggcgct ggtggaagcg cagcgttacc
     1921 ccgaggacta cgacggcatc ctcgccggag ccccggccct gctgccgtcc tccaccctgc
     1981 cgacctgggg ctggatccag cagcaactcc acgcgccggg tgcctatatc tcgaaagaca
     2041 agctgcgggc gatcgccgcg gcgacggtgc gcgcctgcga tgcgcaggat ggcgtcgaag
     2101 acggcgtgat cgacgatccg cgccagtgtc gcttcgatcc cgcggtgctg acctgcgcgg
     2161 gcgaggagaa cgaccagtgc ctgacgccgc cgcaggtgca ggctctgcgc gcaatctatg
     2221 cggggccggg cgcgccgtat cacggcttcg agccgggcgg cgaactcggt gacaagggtg
     2281 gctggcgcga ctggttcacc ggcgacaagc cgcgcgctgc catcgagtac gtctacgtgc
     2341 aggagttcat gcgctacctg gtctacagcg atgcgcaatg gagcgcggac cagttcgacc
     2401 tcaagcgcga tggcgatgcg ttctaccggc agctgcacga catcctcgat gccggcgatc
     2461 ccgacctgtc gaagttcgcc gcacgcggcg gcaagctgat cctgtaccac ggctggaacg
     2521 acggcgcgct gtcgccgcgc accaccatcg attactacga gagcgtgcgt gcgcgcatgg
     2581 gcgcgaagaa ggcgcagcag ttcatcacgc tgtacatggt gccgggcctg cagcactgca
     2641 tcggcgggcc ggggccgaat gctttcggcc agctggcgcc cgggccggtc gccgatccca
     2701 ggcgcaacat cagcgcggcg ctggaggcgt gggtggagca gggtgccgcg ccggggccga
     2761 tcatcgccgg caagtacgac cgcgacatca cgccgctgct cgcgccggaa aaagcctcgc
     2821 tgcaacgcac gcggccgctg tgtccgtatc cgcaggtggc gcgctggagc ggccatggca
     2881 gcagcgacga cgcggcgaat ttctcctgcg tggcgccgaa ataaaacgtc atttccgcga
     2941 aagcgggaat ccatcgtgat gatgtgcgcg cgccagtcgc atggattccc gctttcgcgg
     3001 gaatgacgga gtgcggttga actagaaacc gaacccggca cccagttcgc gcagcgcctg
     3061 caactccgct tcgtgatccg cccgccgcgc cgcgctttcc tgcagcagcg tgttgaggaa
     3121 cgacaggtcc ggctgcgtca gcggcacgtc gaagccgggg atgctcggca gctgcaccgt
     3181 gatgggcagc ggaatcggcg gcggctccgg cagcggcagg gtgatcggcg tttcgccgtt
     3241 gagcagggtg accggcaggc acagtccccc gaagggacct gaaggcacgt cgaacgccgg
     3301 cgtgctcgcg tccggcgcgt tgtcgaaatc cagcgccagc gcgatgttgt tcgacgaggc
     3361 gcgcgcgccc agcggctcga agccgaagcg ccaggccagc atgttgagga tggaattcgg
     3421 atcgaactgc aggtgctcca cgtggccgcg cttggcgcgc gggcccagca gcacgcaggg
     3481 cacgcggcag cccaggcggc cgtcgttgcc cagcgccgct tcggcctcgg tcagcggcgc
     3541 gaacggcggc ggcacgtggt cgtagaagcc gccccactcg tcgtagttga tgatcatcag
     3601 cgtgcgttgc cagtccgggt gcgcgcgcag cacgttgtac acctggttca aaaaatcctg
     3661 gccgtcgcgg atgtcggcgt agggatgatc gtcgaaggaa atggcgccgc cttcgttcat
     3721 ggtgttgccg acgttgtcga cgaacatcag ctcgggcagc gggccggcgg cgacgtcggc
     3781 ggcgaaggac ttcatctcgt agcgttgcga gaagctcttg tacttgctgc cccagaacga
     3841 ggtgtaggaa acgtcggtgt agtagtactt cacgctgcgc ccggcgccga gcatgcggtc
     3901 ccagatggtc ggcagcgtgg agatggtggt ggagttcgcc aggcggtcgg tctgccccgc
     3961 atgcatgtac atgcggttgg gcgtggtcag cgacaggatg ccggagaagt agcggtcgca
     4021 gatggtccag ttttccgcgc agcccttgaa gaacggcagc gaatcggcgg tgtagtagcc
     4081 gatgggaaac ttgtcgcccg gcggctgcgt cagcaggaag ccgtccatct gcccgttgtt
     4141 gatctcggtg cgcccgcccg agtagctgtg gtcgggatcg gccaggttgc agttctggaa
     4201 gttgggcgca aggtcgaacg aatcgtgcgc gttgccgtcg ctgtccttga gcgtggtgcc
     4261 ggcctggcgg ccgtcggcgc cgggcaccca gccgaggaaa tgatcgaacg agcggttctc
     4321 ca
//
LOCUS       DASZZC010000309         6921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_667090,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000309 DASZZC010000000
VERSION     DASZZC010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667090"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VHE37_06410"
     CDS             <1..588
                     /locus_tag="VHE37_06410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT35194.1"
                     /translation="IAMEFIKGHDLTKYVKPTALLPVGDALRYVADAADALDYAHTNN
                     VVHRDIKPANMMLLADSKVIKLMDFGIARMTDSSKTKTGMVLGTPSYMSPEQLAGKKV
                     DGRSDLFSLGVSLYQLLTGQLPFQADSMATLMYKIANEPHTALSTVRPELPAKLNAII
                     DKVLNKDMDKRYQRGNEFARDLRAVIGGMQAAPAK"
     gene            601..1422
                     /locus_tag="VHE37_06415"
     CDS             601..1422
                     /locus_tag="VHE37_06415"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HVT35195.1"
                     /translation="MALKSKVATALLTDMGRVRNNNEDAIAADMDIGLLLLADGMGGY
                     NAGEIASGIAIATITDTAHREWKNLRHGEIDADTGYSVEALMLKGAVEAAHTSIYHVS
                     QTQPQCAGMGTTIVAVILHDDRMSIAYVGDSRLYRFRQNTLEQITRDHSLIEELIARG
                     HYTREEAAKLVRKNIVTRALGVEPDVSVDIIEDVVDVGDILLICSDGLSDMVTDETIG
                     LTLGKYADNLELAVKELVGKALENGGKDNVSVVLARIDGSFTRGRRWYERLMEWF"
     gene            1432..2151
                     /locus_tag="VHE37_06420"
     CDS             1432..2151
                     /locus_tag="VHE37_06420"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVT35196.1"
                     /translation="MGKLSVTDSSGKHSEVLLNKERVTIGRHADNDIPLNDKAVSGHH
                     AVVITILQDSFLEDLDSTNGTQVNGKQVAKHPLSHGDVISIGRNQLKYESDAGGSEDD
                     FEKTMILKPSQLGAAFESQVNKAAAAPAAPGAPAPAPAAGSGAPAKPLLGKLRVASGP
                     NQGKELELSKALTTIGKPGVQVAAITRRADGYYIVHVGGQGAGSRPLVNGAEIDAQAR
                     KLAHNDMIELAGTKMAFLLVS"
     gene            complement(2324..3409)
                     /gene="alr"
                     /locus_tag="VHE37_06425"
     CDS             complement(2324..3409)
                     /gene="alr"
                     /locus_tag="VHE37_06425"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVT35197.1"
                     /translation="MIPRVTARIDLSAIRHNLGVVRQHAPRSRVMAAIKADGYGHGAV
                     PVARALEAAKVDAFAVACLEEAMTLRQAHVQTPIALLEGIISSEEAAQAVYERLQVVV
                     HDYWQLELLQALPATAEVNLWFKLDSGMHRLGFPLGEVPKLVEALKQRPRWRFHGWIT
                     HLASADEPGSDFTTMQIGRFEHALAGVAGPRSIGNSAGLLAWPQTHVDWVRPGLMLFG
                     ASPLPERSAVQLGLRPAMHLESRLIAVREFAAGETIGYGQTYRCSEATRIGVVAVGYA
                     DGLPRRLANGAPLLVRGRRALLAGRVSMDMITVDLRHVPQASVGDHVVLWGTGAPAEE
                     LARYADTLAYELFCGLTQRVRFVYEGG"
     gene            complement(3406..4764)
                     /gene="dnaB"
                     /locus_tag="VHE37_06430"
     CDS             complement(3406..4764)
                     /gene="dnaB"
                     /locus_tag="VHE37_06430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HVT35198.1"
                     /translation="MQNPPKHPPHSLEAEMSVLGGMMLDNRAGQDVLDRLSEADFYIA
                     SHQVIFRGIVQLLSSHAPCDFITLTEHLRHASLLEQAGGIAYLGSLVNDTPSAANVRS
                     YAEIVRERSILRKLIEVGQDTAEMGFRPEGRPPAELLDQAEQKIFALREGDKKSKAQY
                     FEMPPLMDQLEKQLEVLSNSTGGLGGLETGFVDFDKKTGGLRAGDLIIIAGRPGMGKT
                     SLAMNIAENVAIDKKLPAAVFSMEMSAEQLAMRVISSFGRVDQQNLRTGQLTDMDWSR
                     LSSAIGLVREAPLYIDETGALSPLELRARARRIAARHGLSLIVIDYIQLMQVPGSENR
                     TNEISEISRNLKALAKELSCPIIALSQLNRGVESRDNKRPRMSDLRESGGIEQDADLV
                     VFVYRDEYYNENSTERGVAEIIIGKQRNGPTGTVKTAFHGQYTRFDNLAQGDGYGGGG
                     YE"
     gene            complement(4848..5306)
                     /gene="rplI"
                     /locus_tag="VHE37_06435"
     CDS             complement(4848..5306)
                     /gene="rplI"
                     /locus_tag="VHE37_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HVT35199.1"
                     /translation="MEVILLEKIKKLGDLGDTVKVKPGFGRNFLLPQGKALPATEANR
                     KVFETRKAELMKKANDSLSAAKMRAEKLNGKTITVKALAAEEGKLYGSVGPGDVVRAA
                     EAQGLEFKKSELEMTGAIRQIGSYDVVVRLHTEVEAALKVVVVEEKAATA"
     gene            complement(5317..5544)
                     /gene="rpsR"
                     /locus_tag="VHE37_06440"
     CDS             complement(5317..5544)
                     /gene="rpsR"
                     /locus_tag="VHE37_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVT35200.1"
                     /translation="MAAYFRRKKTCKFTSEGAVSIDYKDLATLKQYIGENGKIVPARI
                     TGTKSRYQRQLAVAIKRARYLALLPYTDKHE"
     gene            complement(5547..5960)
                     /gene="rpsF"
                     /locus_tag="VHE37_06445"
     CDS             complement(5547..5960)
                     /gene="rpsF"
                     /locus_tag="VHE37_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVT35201.1"
                     /translation="MRHYEVVVMVHPDQSEQVPAMTERYKKMVTEGGGKIHRLEDWGR
                     RQLAYPIAKAHKAHFLLLNIECDDAVLKELENAFRFNDAVIRKLVIKMEAAETAQSPL
                     FKVPEEERRGEYRARTESAESADGEAADAATEAGA"
     gene            complement(6113..>6921)
                     /locus_tag="VHE37_06450"
     CDS             complement(6113..>6921)
                     /locus_tag="VHE37_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVT35202.1"
                     /translation="IRQHIIERKWICDSGSTTQAWTFGENVSWSSYSVIGGQIYREAG
                     RYRMVSDRIQADVDSTYGGARVLRVTSLDNDAFSASLAGGRTLACKLDPAGFAELAAR
                     YDRQERSLATATAGKAPPGTPPAGAALAARAAEPGVPQAHEPESSSQPARVVEPESSP
                     KPAAPAEMETASFVPADTPPYYDARRVTQPAYPAAAARRHEEGDVLLQLLVGADGHVR
                     QESVLKSSGSPALDSAALAAVRGWDFTAGTHAGKPVDASAQVKVSFHLGN"
BASE COUNT         1203 a         2341 c         2184 g         1193 t
ORIGIN      
        1 atcgccatgg aattcatcaa gggccatgac ctgaccaagt acgtgaagcc gaccgcgctg
       61 ctgccggtgg gcgacgcgct gcgctatgtg gccgacgccg ccgacgcact ggactacgcg
      121 cataccaaca acgtggtcca ccgcgacatc aagcccgcca acatgatgct gctggccgat
      181 tccaaggtga tcaagctgat ggacttcggc atcgcgcgca tgactgattc gtcgaagacc
      241 aagaccggca tggtgctcgg cacgcccagc tacatgtcgc cggaacagct ggccggcaag
      301 aaggtcgatg gccgctctga cctgttctcg ctcggcgtca gcctgtacca gctgctgaca
      361 gggcaactgc cgttccaggc cgattccatg gccactctga tgtacaagat tgccaacgag
      421 ccgcacaccg cgctcagcac cgtgcggccc gaactgccgg ccaagctcaa cgccatcatc
      481 gacaaggtgc tgaacaagga catggacaag cgctaccagc gcggcaacga gttcgcccgc
      541 gacctgcggg cggtgattgg cggaatgcag gctgcgccag ccaaataagc gagtccaagg
      601 atggctctga aaagcaaagt cgcaactgcg ctgctcaccg acatgggacg ggtgcgcaac
      661 aacaacgagg acgcgatcgc cgcggacatg gacattggcc tgctgctgct ggccgacggc
      721 atgggcggct acaacgccgg cgagatcgcc agcggcatcg ccatcgccac cattaccgat
      781 accgcccacc gcgaatggaa gaacctgcgc cacggcgaaa tcgatgccga taccggctac
      841 agcgtcgagg cgttgatgct caagggcgcg gtcgaggcgg cgcatacctc gatctatcac
      901 gtgtcgcaga cccagccgca gtgcgcgggc atgggcacca ccatcgtcgc ggtgatcctg
      961 cacgacgacc gcatgtcgat cgcctacgtc ggcgactcgc ggctgtaccg cttccgccag
     1021 aacacgctcg aacagatcac ccgcgatcac tcgctgatcg aagaactgat cgcgcgcggc
     1081 cactacacgc gcgaggaggc ggccaagctg gtgcgcaaga acatcgtcac ccgcgcgctg
     1141 ggcgtggagc cggacgtttc ggtggacatc atcgaagacg tggtcgacgt gggcgacatc
     1201 ctgctgatct gctcggatgg cctcagcgac atggtgaccg atgagacaat tggcttaaca
     1261 cttggcaaat atgctgataa tcttgaactc gcagtcaaag agctggtggg aaaagccctt
     1321 gagaatggcg ggaaggacaa tgtttcggtc gtgctggcgc gcatcgatgg ctcattcacg
     1381 cgcggtcgcc gctggtacga acgtttaatg gaatggttct agagaactct tatgggcaag
     1441 ctttccgtca cagacagctc aggcaagcac tccgaggtcc tgctgaacaa ggagcgcgtg
     1501 accatcggcc gtcacgccga taacgacatc ccgctcaacg acaaggcggt gtcgggccat
     1561 cacgccgtgg tcatcaccat cctgcaggac tcgtttctcg aagacctcga cagcaccaac
     1621 ggcacccagg tcaacggcaa gcaggtggcc aagcatccgt tgtcgcacgg cgacgtcatc
     1681 tccatcggcc gcaaccagct gaagtacgaa agcgatgccg gcggcagcga ggatgatttc
     1741 gagaagacca tgatcctcaa gccgagccag ctcggcgccg ctttcgaatc gcaggtgaac
     1801 aaggctgccg cggcacctgc ggcacccggc gcgcccgcgc cagcccctgc ggcgggcagc
     1861 ggagcaccgg ccaaaccgct gctgggcaag ctgcgcgtag ccagcggccc gaaccagggc
     1921 aaggaactgg agttgtcgaa agcccttacc accatcggca aacccggtgt gcaggtggcg
     1981 gccatcacgc gccgcgccga cgggtactac atcgtgcatg tcggcggcca gggcgcgggc
     2041 agccgcccgt tggtcaatgg cgcagagatc gacgcgcagg cacgcaagct cgcgcacaac
     2101 gacatgatcg aactggccgg caccaagatg gcgttcctgc tggtcagctg agcgttcgac
     2161 gcgacgctgg cgtcgcaaaa tgcacctgaa tgccggtcac cgcaccggca tttttgttgt
     2221 tactaattaa tgtataaagt taaataaata caataattat taacagcacc aggcggggtg
     2281 cggtgaaggc aaaaagccgg cccggaaagc cgccgacgat acctcagccg ccctcgtaga
     2341 cgaagcgcac tctctgcgtg agtccgcaga acagttcata cgccagcgta tccgcgtacc
     2401 gggccagttc ctcggcgggc gctcccgtgc cccacaacac gacgtgatcg ccgacgctgg
     2461 cctgcggcac gtggcgcaga tcgacggtga tcatgtccat cgacacccgc ccggcgagca
     2521 gtgcgcggcg cccgcgcacc agcagcggcg cgccattggc cagccggcgc ggaaggccgt
     2581 ccgcgtaacc gaccgccacc acaccaatcc gtgtcgcctc cgagcaacgg taggtttgcc
     2641 cgtagccgat ggtctcgccc gcggcaaact cgcgcaccgc gatcagccgg ctctccaggt
     2701 gcatcgccgg cctcaacccc agttgcacgg cgctccgctc aggcagcggc gaggcgccga
     2761 acagcatcag cccgggccgc acccagtcca cgtgcgtctg cggccacgcc agcaggcccg
     2821 cggaattgcc gatggagcgc gggccggcca cgccagccag cgcatgctcg aagcggccga
     2881 tctgcatcgt ggtgaaatcg ctgcccggtt cgtcggcgct cgccaggtgc gtgatccagc
     2941 cgtggaaacg ccagcgtggc cgttgcttca gcgcctccac cagcttcggc acttcgccca
     3001 acggaaaccc caggcggtgc atgccgctgt cgagcttgaa ccacaggttc acctcggccg
     3061 tcgccggcaa ggcctgcagc agttccagct gccagtagtc gtgcaccacc acctgcaggc
     3121 gctcgtatac cgcctgcgcc gcttcctcgc tggagatgat gccctccagc agcgcgatgg
     3181 gcgtctgcac atgcgcctgg cgcagcgtca tcgcctcttc caggcaggcg acggcaaagg
     3241 catcgacttt cgccgcctcc aacgcgcgcg ccaccggcac cgcgccgtgg ccgtagccat
     3301 cggccttgat cgcggccatc acgcgcgagc gcggcgcgtg ctgccgcacc acgccaaggt
     3361 tgtgccggat ggcggacagg tcgatccgcg ccgtgacccg cgggatcatt cgtacccgcc
     3421 gccgccgtag ccgtcgcctt gcgccaggtt gtcgaagcgc gtgtactgtc catggaacgc
     3481 ggtcttcacc gtgccggtcg ggccgttgcg ctgcttgccg atgatgatct cggccacgcc
     3541 acgctcggtc gagttctcgt tgtagtactc gtcgcggtag acgaacacca ccaggtccgc
     3601 gtcctgctcg ataccgccgg attcacgcag atcgctcatg cgcgggcgct tgttgtcacg
     3661 actctccacg ccgcggttca gctgcgacag cgcgatgatc gggcagctca gttccttggc
     3721 cagcgccttc aggttgcgcg agatctcgga aatctcgttg gtgcggttct ccgaacccgg
     3781 cacctgcatc agctggatgt aatcgatgac gatcagcgac aggccatggc gggccgcgat
     3841 gcgccgcgcg cgcgcgcgca gctcgagcgg cgacagcgcg ccggtctcgt cgatatacaa
     3901 cggtgcttcg cgcaccaggc caatggccga cgacagccgc gaccagtcca tgtcggtgag
     3961 ctggccggtg cgcaggttct gctggtcgac gcggccgaac gacgagatga cgcgcatggc
     4021 cagctgctcc gccgacattt ccatcgagaa cacggcggcg ggaagcttct tgtcgatcgc
     4081 cacattctcg gcgatgttca tggccaggct ggtcttgccc atgccgggac ggcccgcgat
     4141 gatgatcagg tcgccggcgc gcagcccgcc ggttttcttg tcgaagtcga cgaagccggt
     4201 ctcgagcccg cccagtccgc cggtgctgtt gctgagcacc tcgagctgtt tttccagctg
     4261 gtccatcagc ggcggcatct cgaagtactg cgccttcgat ttcttgtcgc cctcgcgcag
     4321 cgcgaagatc ttctgctcgg cctggtccag cagttcggcg ggcggacgac cttccgggcg
     4381 gaaacccatc tccgcggtgt cctgccccac ctcgatcagc ttgcgcagga tggaacgctc
     4441 gcggacgatt tccgcgtagg agcgcacgtt ggccgcactc ggcgtgtcgt tgaccagcga
     4501 acccaggtag gcaatgccgc cggcctgctc cagcaggctg gcatggcgca ggtgctcggt
     4561 cagcgtgatg aagtcgcatg gcgcatgcga cgacagcagc tgcacgatgc cgcggaagat
     4621 gacctggtgc gaagcgatgt agaaatcggc ctcggacagg cgatcgagca cgtcctgccc
     4681 ggcgcggttg tcgagcatca tgccgccgag caccgacatt tcggcttcca gcgaatgcgg
     4741 tggatgtttg ggcggattct gcacggtgag cggaaaagaa aacggcccgc tgtgcgcggg
     4801 ccgtcagttt acttccaggt gacgttgctt gcagcgcggt cagtgcatca ggccgtcgcg
     4861 gccttctcct cgaccaccac caccttgagc gcagcctcga cctcggtgtg caggcgcacc
     4921 accacgtcgt agctgccgat ctggcggatt gcgccggtca tctcgagttc gctcttcttg
     4981 aactccaggc cctgcgcctc ggccgcacgc accacgtcgc cgggaccgac cgagccgtac
     5041 agcttgcctt cctcggccgc cagcgccttg accgtgatgg tcttgccatt gagcttctcc
     5101 gcgcgcatct tcgccgcgga gaggctgtcg ttggccttct tcatcagttc ggccttgcgc
     5161 gtctcgaaca ccttgcggtt ggcctcggtg gccggcagcg ccttgccctg cggcagcagg
     5221 aaattgcgtc cgaagccggg cttgaccttg accgtgtcgc ccaggtcgcc gagcttcttg
     5281 atcttctcga gcaggatcac ttccatggtc ggctccttac tcgtgcttgt cggtgtaagg
     5341 cagcagcgcg aggtagcgcg cgcgcttgat cgccaccgcc agctggcgct ggtagcggct
     5401 cttggtgccg gtgatgcggg ccggcacgat cttgccgttc tcgccgatgt actgcttcag
     5461 cgtggccaga tccttgtagt cgatgctcac cgcgccttcg ctggtgaact tgcaggtctt
     5521 cttgcggcgg aaataagcgg ccatgttcag gctccggctt cggtggcggc gtcggcggct
     5581 tcgccgtcgg cggattcagc ggactcggtg cgcgcgcggt actcgccgcg gcgctcctct
     5641 tccggcacct tgaacagcgg cgactgcgcg gtttccgcag cctccatctt gatcaccagc
     5701 ttgcggatca ccgcgtcgtt gaagcggaac gcgttctcca gttccttgag cacggcatcg
     5761 tcgcactcga tgttcagcag caggaaatgc gccttgtgcg ccttggcgat cgggtaagcc
     5821 agctggcgcc ggccccagtc ctcgagacgg tggatcttgc cgccgccctc ggtgaccatc
     5881 ttcttgtaac gctcggtcat ggccggaacc tgttcgctct ggtccgggtg caccatgacg
     5941 acgacttcgt aatgtcgcat tgcaactcct tcctgtggac tcgcgcacct ggcgcgaggc
     6001 tccccgccaa tcgataactg caagcggtgg agcaagtgga taaaaccccg ggctggcccg
     6061 aggggcgcgc atcttaaggg atcgccccca gccgcgcaac gcgggccgcg cctcagttgc
     6121 ccaggtggaa actgaccttg acctgggcgc tggcatccac cggtttgccg gcgtgggtgc
     6181 cggcggtgaa atcccagccg cgcaccgctg ccagcgcggc gctatcgagc gccggcgagc
     6241 cgctcgattt gagcaccgac tcctgccgca cgtgcccgtc cgcgcccacc aacagctgca
     6301 gcagcacgtc gccttcttcg tgacgtcgcg cggccgcagc cggataggcc ggctgcgtca
     6361 cccggcgcgc gtcgtagtac ggcggcgtgt cggccggcac gaacgaagcg gtttccatct
     6421 ccgccggcgc cgccggcttc ggcgagctct cgggctccac aaccctggca ggctgagacg
     6481 aggattcggg ctcatgcgcc tgcggcacgc cgggctccgc cgcgcgagct gcaagcgcag
     6541 ctccagccgg cggcgtcccc ggcggcgcct tgccagcggt ggcggtggcc aggctgcgct
     6601 cctgcctgtc gtagcgcgcc gccagttccg caaatccggc agggtcgagc ttgcaggcca
     6661 gcgtgcgtcc gccggccagg ctggccgaga acgcgtcgtt gtccaggctg gtcacgcgca
     6721 gcacgcgcgc gccgccatag gtgctgtcaa catcggcctg gatgcggtcg ctgaccatgc
     6781 gataacggcc ggcctcgcgg tagatctgcc cgccaatcac gctgtacgac gaccagctta
     6841 cgttctcgcc gaaagtccag gcctgcgtcg tgctgccgct gtcgcagatc cacttgcgct
     6901 cgatgatgtg ctggcggatt t
//
LOCUS       DASZZC010000310         8694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1244482,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000310 DASZZC010000000
VERSION     DASZZC010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244482"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VHE37_06455"
     CDS             complement(<1..286)
                     /locus_tag="VHE37_06455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT35203.1"
                     /translation="MTVPATQPEIMQQPPAVVPRVSAVMPCLNEERTLEACIRKAQAS
                     FARLGVAGEVVIADNGSTDRSRELAAALGARVILEQVKGYGAAVAAGVNAA"
     gene            465..2144
                     /locus_tag="VHE37_06460"
     CDS             465..2144
                     /locus_tag="VHE37_06460"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVT35204.1"
                     /translation="MPDTPQTRYIFVTGGVVSSLGKGIAAASLGAILESRGLKISMIK
                     LDPYINVDAGTMSPFQHGEVFVTEDGAETDLDLGHYERFLRGKTSRHSNVTTGQIYRN
                     VLEKERRGDYLGSTIQVIPHITDEIQNRIRLGAAGADICMVEIGGTVGDIESLPFLEA
                     IRQFGVEVGEKNVMYMHLTLVPYIRSAGEMKTKPTQHSVKELRSIGIQPDVLLCRTEK
                     PIPDGERRKIALFTNVPQRAVISAVDLDDIYKIPAWLNAQGLDELVVEHFGIECRKAD
                     LSQWERVVAARSQQDVTVQIAMVGKYVDLADAYKSLNEALAHAGLHTQTQVKIRYIES
                     ETVESQGARVLQGMDAILVPGGFGERGIEGKIAAARYARENKIPYLGICLGMQVAVIE
                     YARDVCGLKDAHSTEFNPQTPHPVIALVTEWRDAAGKVHLRTASSNKGGTMRLGVQSC
                     RLKPGSKTRALYNAEVITERHRHRFEFNNNYKALLTEKGMDLVAEAVENELVEMIELP
                     DHPWFIGCQFHPEFTSTPRDGHPLFEGFIRAAREHHLTNQASAKTVKAQAA"
     gene            2141..2977
                     /gene="kdsA"
                     /locus_tag="VHE37_06465"
     CDS             2141..2977
                     /gene="kdsA"
                     /locus_tag="VHE37_06465"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HVT35205.1"
                     /translation="MKLCGHEIGLEHPLFLIAGPDTLESQQLALDVAGHIAPLAKKLG
                     ILYIFKGSFDKANRSSHKSYRGPGLEGGLKIMQAVQKQIGVPVLTDVHEDTPLAEVAS
                     VIDVIQTPAFLCRQTNFMQNVARTGRPINVKKGQFMSPWEMGNVIEKMKAVAPHQFML
                     CERGFSFGYNNLVADMRGLAIMRKYAPVVFDSTHTVQLPGGQGNASGGMREMIPVLAR
                     AAVGAGVSGVFMETHPKPDEALCDGPNSLPLKHIGGLLDTLVELDRTVKKSGLLEQQL
                     TS"
     gene            2990..4273
                     /gene="eno"
                     /locus_tag="VHE37_06470"
     CDS             2990..4273
                     /gene="eno"
                     /locus_tag="VHE37_06470"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HVT35206.1"
                     /translation="MSKIAHVAALEIIDSRGNPTVEAKVTLASGATGRAAAPSGASTG
                     MREAVELRDGDKKRYLGKGVSKAVKNANTEIAKAVTGMEAQEQAALDARMIELDGTDN
                     KGRLGANAILAVSLANAHAAAAEKGLPLYRHLGGISSVTLPVPMMNVINGGAHADNNV
                     DIQEFMILPVGAPSFSEALRYGAEVFHTLKKVLHERKLNTAVGDEGGFAPNLESNVAA
                     LDCLLEAIDRAGYKAGKDIYLGLDVASSEFFEKGKYVLHGEGKSYTPEQFGDFLAGIC
                     DKYPVISIEDGCAEGDWKGWKYLSEKLGDRVQLVGDDLFVTNTRILAQGIDQGIANSI
                     LIKPNQIGTLTETLAAIQMATEARYASVVSHRSGETEDATIADIAVGTTATQIKTGSL
                     CRSDRMAKYNQLLRIERELGARAHYPGAAAFPVSI"
     gene            4277..4555
                     /locus_tag="VHE37_06475"
     CDS             4277..4555
                     /locus_tag="VHE37_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HVT35207.1"
                     /translation="MIRRAAIVTLMLMLAGLQWRLWLADGGITHTQRLGAQAEIQQAE
                     NQRLVARNAALDAEVTDLKSGTAAIEARARTTLGMIRQNETFYLVVQP"
     gene            4563..5276
                     /gene="ispD"
                     /locus_tag="VHE37_06480"
     CDS             4563..5276
                     /gene="ispD"
                     /locus_tag="VHE37_06480"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HVT35208.1"
                     /translation="MSSTTARYWVVIPAAGGGSRMGLARPKQYAALRGRALLEWSIAP
                     FLDTEWIDGVVLVLARSDTEFARLAVARHPKIVTTVGGSARADSVLSGLEAVRERSAG
                     FSEVYVMVHDAARPCLMWSDLENLRDEASDEQGGLLAVPATDTLKKSKQGQVSSTVDR
                     AFIWRAQTPQLFRLDLLLHALSECAERGLGVTDEASAMERAGYKPRLVRGRESNIKVT
                     YPEDLPLAEFWLARQEQQP"
     gene            5273..5761
                     /gene="ispF"
                     /locus_tag="VHE37_06485"
     CDS             5273..5761
                     /gene="ispF"
                     /locus_tag="VHE37_06485"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HVT35209.1"
                     /translation="MSEVAFRIGHGYDAHRIEAGDGVMLGGVRIACEWRVIAHSDGDV
                     LIHALCDALLGAVGGGDIGRLFPDTDAQYRGISSLHFLHETMRRVREQGWGVGNADLT
                     LVAQVPRVAPHAGAIRDTLALALGVGSGCVNLKATTTEGMGYIGRREGIAAHAVVLLQ
                     RA"
     gene            5811..6446
                     /locus_tag="VHE37_06490"
     CDS             5811..6446
                     /locus_tag="VHE37_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT35210.1"
                     /translation="MLEAVLNPTPTRKRGSRAGRDTREEILNAAEELLQRRGYNAFSY
                     QHIAVQLGIRNAAIHYHFPAKEDLGVALVRRYRDRFREWCEQTNRLDNAWDRLSAYFQ
                     TYVDFLDDQCKICPAGVLGSEYEAIPEEMRQEARLLMREIFEWLVETLDHGRAHGTLQ
                     FPGDSRHKAVEIGAALQGGLQIARMAGQERFHQVLAQLGLELAQPSANPMP"
     gene            complement(6850..7383)
                     /locus_tag="VHE37_06495"
     CDS             complement(6850..7383)
                     /locus_tag="VHE37_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HVT35211.1"
                     /translation="MAPDEDDDAALFREAMKGVQRKGEARRAGAPRRAVSARPLQRKA
                     DERAVLQEMLLDPDPESMESGDTLSYRLSGVQDAVMRKLRRGQYALQAEVDLHGMNRR
                     QAQHALAGFLALCQDRGYRCVRVIHGKGLGSPNSGPVIKRLVDSWLRRRSDVLAFCSA
                     RPADGGTGALYVLLRSA"
     gene            7438..8196
                     /gene="surE"
                     /locus_tag="VHE37_06500"
     CDS             7438..8196
                     /gene="surE"
                     /locus_tag="VHE37_06500"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HVT35212.1"
                     /translation="MRILLSNDDGCMAPGLRCLHGHLQKHHQVTVVAPDRNRSGASNS
                     LTLLNPLRVQTHADGVICVDGTPTDCVYLALTGHLRGSFEGAPDMVVSGINAGANLGD
                     DTLYSGTVAAAMEGRHLGYPAIAVSSVGAVPEHYDTAARVTLRLIEHLQKDPLPGDTI
                     LNVNVPDVPFEQLRGYEVTRLGNRHRAEGVTRAQDPRGRTIYWIGSTGGERDCGQGTD
                     FHAVAHHRVALTPLTVDLTRHDALSRLAQWAGAL"
     gene            8193..>8694
                     /locus_tag="VHE37_06505"
     CDS             8193..>8694
                     /locus_tag="VHE37_06505"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVT35213.1"
                     /translation="MNAPRAHARVAEPPARSRLVDELRRQGINDEKVLAAIARVPRHE
                     FVDGALQSRAYENNALPIGHAQTISQPFVVALMTQALTHGRRLRRVLEIGTGSGYQTA
                     VLAGLVDTVFTVERIKPLTEIARKRLAAQGCKNVHFGYADGSKGWSAYAPYDGIMVTA
                     AADSVPE"
BASE COUNT         1592 a         2846 c         2822 g         1434 t
ORIGIN      
        1 gggccgcatt cacacccgcc gcaaccgcgg cgccataacc cttgacctgt tcgaggatca
       61 cgcgcgcgcc gagcgcagcc gcgagttcgc gcgagcgatc ggtggagccg ttgtcggcga
      121 ttacgacttc gccagccaca cccagccggg cgaagctcgc ctgggccttg cggatgcagg
      181 cctccagagt gcgttcttcg ttcaggcaag gcatcaccgc cgaaaccctg ggaacaactg
      241 cgggcggctg ctgcatgatc tcgggctgag tggctggaac ggtcatcgga cgacgctgga
      301 atatccccgg aagtaccgac agcgggtgtt gccgctgtct tggtgcgcac gtagtcagcc
      361 gattatcgca gaggcggcaa gtccagcggg cagggcgtgc gcggggctcg gcgttgtcgc
      421 tcggataccg atggcttaaa attggaaaaa cccagacacc cgccatgccc gataccccgc
      481 agactcgcta tattttcgta accgggggcg tggtgtcctc gcttggcaag ggtatcgctg
      541 cggcgagcct tggcgcgatc ctcgaatcgc gcggcctcaa gatcagcatg atcaagctgg
      601 acccgtacat caacgtcgat gccgggacca tgagcccgtt ccagcacggc gaggtgttcg
      661 tcaccgagga cggcgccgaa acggacctcg acctgggcca ctacgagcgc ttcctgcgag
      721 gcaagaccag ccggcacagc aacgtgacca ccggtcagat ttaccgcaac gtgctggaaa
      781 aggagcgccg aggcgattac ctcggcagca ccatccaggt tatcccgcat atcactgacg
      841 agatccagaa ccgcatccgc ctgggcgcgg ctggcgcgga catctgcatg gtggagatcg
      901 gcggcacggt cggcgacatc gaatcgctgc cgttcctcga ggccatccgc cagttcggtg
      961 tggaagtggg tgaaaagaac gtcatgtaca tgcacctgac cctggtgccg tacattcggt
     1021 cagccggcga gatgaagacc aagccgaccc agcactcggt gaaggagctg cgctccatcg
     1081 gcatccagcc cgacgtgctg ctgtgccgca cggagaagcc catccccgat ggtgagcggc
     1141 gcaagattgc gctgttcacc aatgtgccgc agcgggcggt gatttccgcc gtcgacctgg
     1201 atgacatcta caagattccg gcctggctca acgcgcaggg actggacgag ctggtggttg
     1261 aacatttcgg catcgaatgc cgcaaggccg acctgtcgca gtgggaacgc gtggttgctg
     1321 cacgcagcca gcaggatgtg acagtgcaga tcgccatggt cggcaagtac gtcgatctgg
     1381 ccgacgccta caaatcgctc aacgaagcgc tggcgcatgc cggcctgcac acgcagacgc
     1441 aggtgaagat ccgctacatc gaatcggaga cggtggagtc gcagggcgca cgcgtgctgc
     1501 agggcatgga cgcgatcctg gtgccgggcg gcttcggcga acgaggtatc gagggcaaga
     1561 tcgccgccgc gcgttatgcg cgcgagaaca agatccccta cctgggcatc tgcctgggca
     1621 tgcaggtggc ggtgatcgaa tacgcgcgcg atgtgtgtgg gctcaaggac gcgcacagca
     1681 ccgagttcaa cccgcagacg ccgcacccgg tgattgcgct ggtcaccgaa tggcgtgacg
     1741 ctgcgggcaa ggtgcacctg cgcactgcca gctccaacaa gggtggcacc atgcgcctgg
     1801 gcgtgcagtc ctgccggctc aagccgggca gcaagacgcg cgcgctgtac aacgcggagg
     1861 tcatcaccga gcgtcaccgc caccgctttg agttcaacaa caactacaag gccttgttga
     1921 cggaaaaagg catggacctg gtggcggagg cggtggagaa cgaactggtg gaaatgatcg
     1981 agctgccgga tcacccgtgg ttcatcggct gccagttcca tcccgagttc acctcgacgc
     2041 cgcgtgacgg ccacccgctg tttgaaggct tcatccgcgc cgcgcgcgag caccacctga
     2101 ccaaccaggc ttcggccaag acggtcaagg cgcaggccgc atgaaactct gcggacatga
     2161 gatcggcctc gaacatccgc tgttcctgat cgccggcccg gatacgctcg aatcgcagca
     2221 gctggcgctc gatgtggccg gtcatatcgc gccgctcgcc aagaagcttg gcatcctcta
     2281 catcttcaag ggcagtttcg acaaggccaa ccgcagctcg cataagtcct atcgcggtcc
     2341 ggggctggaa ggcggcctca agatcatgca ggcggtgcag aagcagatcg gcgtgccggt
     2401 gctgaccgat gtgcacgagg acacaccgct ggctgaagtg gccagcgtga tcgacgtgat
     2461 ccagacgccg gccttcctct gccgccagac caatttcatg cagaacgtgg cgcgcaccgg
     2521 tcgcccgatc aacgtcaaga aaggccagtt catgtcgccc tgggaaatgg gcaacgtgat
     2581 cgagaagatg aaggccgtcg cgccccatca gttcatgctg tgcgaacgtg gcttctcgtt
     2641 cggctacaac aaccttgtgg cggacatgcg cgggctggcc atcatgcgca agtacgctcc
     2701 cgtggtgttc gattccacgc acaccgtgca gctcccgggc ggacagggca atgcctccgg
     2761 cggcatgcgc gagatgatcc cggtgctggc gcgtgcagcg gtcggtgccg gcgtctccgg
     2821 cgtgttcatg gaaacgcacc ccaagcccga cgaagcgctg tgcgacgggc cgaattcgct
     2881 gccgctcaag cacatcggcg gtttgctcga tacgctggtg gagctggatc gcacggtcaa
     2941 gaaatccggc ctgctcgaac aacaactcac ttcctgaaaa ctcccactca tgtcgaagat
     3001 tgcacacgtc gccgccctgg aaatcatcga ttcgcgcggc aatcccacgg tagaagccaa
     3061 ggtcacgctg gcctccggcg ccactggccg cgccgctgca cccagcggcg cttccaccgg
     3121 tatgcgcgaa gcggttgaac tgcgcgatgg cgacaagaag cgctatctgg gcaagggcgt
     3181 gagcaaggcg gtgaagaacg ccaacaccga gatcgccaag gccgttaccg gcatggaagc
     3241 gcaggagcag gcggcgctcg acgctcgcat gatcgaactc gacggcaccg acaacaaggg
     3301 ccgtctgggc gccaatgcca tcctcgccgt gtcgttggcc aacgcgcatg cggcggcagc
     3361 ggagaaaggg ttgccgctgt accgtcacct gggcggcatc tccagcgtga cgctgccggt
     3421 gccgatgatg aacgtgatca acggcggtgc gcatgccgac aacaatgtcg acatccagga
     3481 attcatgatc ctgccggtgg gtgcgccaag tttttccgag gcgctgcgct acggtgccga
     3541 agtgttccac acgctgaaga aggtcttgca cgagcgcaag ctcaacaccg cggtaggcga
     3601 cgagggcggc tttgcgccga acctcgaatc caatgtcgcc gcgctcgact gcctgctgga
     3661 agccatcgac cgcgctggct acaaggccgg caaggacatc tacctgggcc tcgacgttgc
     3721 gtcgtccgaa ttcttcgaga agggcaagta cgtgctgcac ggcgaaggca agagctacac
     3781 gcccgaacag ttcggcgact ttctcgccgg catctgcgac aagtacccgg tgatttccat
     3841 cgaggatggt tgcgccgagg gcgactggaa gggctggaag tatctttccg agaaactcgg
     3901 tgaccgcgtg caactggtcg gcgacgacct gttcgtcacc aacaccagga tcctcgcgca
     3961 gggtatcgac cagggcatcg ccaattccat cctgatcaag cccaaccaga tcggcacact
     4021 gaccgagacg ctggcggcga tccagatggc gaccgaagcg cgctacgcct cggtggtctc
     4081 gcaccgctcg ggcgaaaccg aggacgccac catcgcggat attgccgtgg gcacgaccgc
     4141 cacgcagatc aagaccggct cgctgtgccg ctccgaccgc atggccaagt acaaccagct
     4201 gctgcgcatc gagcgtgagc tcggtgcgcg cgcccattac cccggcgctg ccgcgttccc
     4261 ggtttccatc tagcagatga tccgccgcgc tgccatcgtc acgctcatgc tgatgctcgc
     4321 cggcctgcag tggcggctgt ggctggccga cggcggcatc acacatacgc agcgcctggg
     4381 ggcgcaggcg gaaatccagc aggcggagaa ccagcggctg gttgcgcgca acgctgcgct
     4441 cgacgccgag gtgaccgatc tcaagtccgg caccgccgcc atcgaagcac gcgcccgcac
     4501 cacgctgggc atgatccggc agaacgagac gttctacctc gtcgtccagc cctgaagcgc
     4561 acttgagcag caccaccgct cgttactggg tcgtgattcc cgccgccggc ggcggttcgc
     4621 gcatgggtct ggcgcggccc aagcagtacg cggccctgcg tggtcgcgcc ttgctcgaat
     4681 ggagcatcgc gccgttcctc gatacggaat ggatcgacgg cgtggtgctg gtgctggccc
     4741 gttccgacac cgaatttgcc cggctggcgg tcgcgcgcca ccccaagatc gtcaccaccg
     4801 tcggcggttc ggcgcgggcg gactcggtgc tgtcgggcct ggaggcggtg cgtgaacgct
     4861 cagccggatt ttccgaggtc tatgtgatgg tgcacgacgc cgcgcgcccg tgcctgatgt
     4921 ggagcgacct ggaaaacctg cgcgacgagg cgtccgacga gcagggcgga ctgctggcgg
     4981 tgccggcgac cgataccctg aaaaaatcca agcagggcca ggtgtcgagc acggtggacc
     5041 gcgcgttcat ctggcgtgcg cagacgcccc agctgttccg cctggacctg ctgctgcacg
     5101 cactgagcga atgcgccgag cgcgggctgg gtgtgaccga cgaggccagc gccatggaac
     5161 gcgccggcta caagccgcgg ctggtgcgtg gccgcgagag caacatcaag gtcacctatc
     5221 ccgaagacct gccgctggcg gaattctggc tggcgcggca ggagcagcag ccgtgagcga
     5281 ggtggcgttc cgcatcggcc atggatacga cgcacaccgc atcgaggccg gcgacggcgt
     5341 gatgctcggc ggcgtgcgca tcgcctgcga atggcgcgtc atcgcgcatt ccgacggcga
     5401 cgtgctgatc catgccttgt gcgatgcctt gctcggtgcc gttggcggcg gcgatatcgg
     5461 ccggctgttt cccgataccg atgcgcaata ccgcggcatc tccagcctgc acttcctgca
     5521 cgaaaccatg cgccgcgtgc gcgagcaggg ctggggcgtg ggcaatgccg acctgacgct
     5581 ggtcgcgcag gtgccgcgcg tggcgccgca tgcaggcgcc atccgcgaca ccctggccct
     5641 tgcgctcggc gtgggttcgg gctgcgtcaa cctcaaggcg accaccaccg agggtatggg
     5701 gtatatcggc cggcgcgagg gcattgccgc gcacgccgtg gtcttgttgc agcgcgccta
     5761 gattttgctg cgcattggcg cggcgtcggc agccggccta cactgcgccg atgctggaag
     5821 ccgtgctcaa tccgacgccc acccgcaagc gcggttcacg cgccgggcgt gatacgcgcg
     5881 aagagattct caacgcggcc gaggaactgc tgcagcggcg tggctacaac gcattttcgt
     5941 accagcacat tgccgtgcag ctgggcatcc gcaacgccgc catccactat cattttccag
     6001 ccaaggaaga cctcggtgtg gcgctggtgc ggcgctaccg cgaccgtttc cgcgagtggt
     6061 gcgaacagac caatcgcctc gacaacgcct gggaccgcct ctcggcgtat ttccagacct
     6121 acgtcgattt cctcgacgac cagtgcaaga tctgcccggc tggcgtgctc ggcagcgagt
     6181 acgaggccat ccccgaggag atgcgccagg aagcgcgcct gctgatgcgc gagatcttcg
     6241 agtggctggt ggagacgctg gatcatgggc gtgcgcacgg cacgctgcag tttcccggcg
     6301 attcgcgcca caaggcggtg gagatcggcg cggcgctgca aggcggattg cagatcgcgc
     6361 gcatggcggg gcaggagcgc tttcaccagg tgctcgcgca actcgggctg gaactggcgc
     6421 agccgtccgc caacccgatg ccctgaattt ccgcctcgtt gcgggtgcag acagtcaacc
     6481 gcaagccttc ttgttgtgtt ataaaatata ttataaattt aaaattagct aatatttaat
     6541 aacaactaaa ttgccattgc ggtgcctggt ttttgagccg ccctgtgata tgaatcttgt
     6601 ttttattcaa ttgaatgagg catttttcac ataaacttac ttcttagtaa gtaaaatgcc
     6661 gaacattcgg gattcgcttc actccaatga accgatccct gccactgaat tttgaaccat
     6721 ggaatccatg cctatccacg acgcggtcag ccatgcaccc gccattccgg tgccggactg
     6781 gcgcacgcgg ctgctcgact ggtggcgccg cctgcgccgc tacggttgag caatatttaa
     6841 ccagcgcgat tacgcgctgc gcagcaggac gtagagcgcg ccggtgccgc cgtcggccgg
     6901 gcgcgcggag cagaaggcca gcacgtcgct ccgccggcgc agccagctgt cgaccagccg
     6961 cttgatcacc gggccgctgt tgggcgaacc caggcccttg ccgtggatca cgcgcacgca
     7021 gcggtagccg cggtcctggc acaacgcgag aaagcccgcc agtgcatgct gcgcctggcg
     7081 gcggttcatg ccgtgcagat ccacttccgc ctgcagcgcg tattgcccgc gccgcagctt
     7141 gcgcatcacc gcgtcctgca cgccggacag gcggtagctg agcgtgtcac cggactccat
     7201 cgattccgga tcgggatcga gcagcatctc ctgcagcact gcgcgctcgt cggctttgcg
     7261 ctgcagcggc cgcgccgaca ccgcgcgccg cggcgccccg gcacggcgcg cttcgccctt
     7321 gcgctgcacg cctttcatcg cctcgcggaa caaggcggca tcgtcgtctt cgtcgggcgc
     7381 cacgcgcggt cggaaaatcg tgctgggtta aactagccga atcaccagat tcgaagcatg
     7441 cgcatcctat tatcgaatga cgacggctgc atggcgccgg gcttgcgctg cctgcatggt
     7501 cacctgcaga agcaccatca agtcaccgtg gtcgcgcccg accgcaaccg cagcggggcc
     7561 agcaactcgc tcacgctgct caacccgctg cgcgtgcaga cgcatgccga cggcgtgatc
     7621 tgcgtcgacg gcacgcccac cgactgcgtc tacctggcgc tcactggcca cctgcgcggc
     7681 agcttcgagg gtgcgcccga catggtggtg tcgggcatca acgccggcgc caacctgggc
     7741 gacgacacgc tttattccgg cactgtggcc gcggcgatgg aaggccgcca tctcggttat
     7801 cccgccatcg cggtgtcgtc ggtgggcgcg gtgccggagc actacgacac cgctgcgcgc
     7861 gtcacgctgc ggctgatcga acacctgcag aaggatccgc tgcccggcga caccattctc
     7921 aatgtcaacg tgcccgacgt gccgttcgag cagctgcgcg gctacgaagt cacccgcctg
     7981 ggcaaccggc accgcgccga aggcgtgacc agggcgcagg acccgcgcgg gcgcaccatc
     8041 tactggatcg gctccaccgg cggcgagcgc gactgcggcc agggcaccga cttccacgcg
     8101 gtggcccacc accgcgtggc gctgacgccg ctcaccgtcg acctcacgcg ccatgacgcg
     8161 ctgtcgcgcc tcgcgcaatg ggcgggcgcg ctgtgaacgc gccgcgcgcc cacgcccgcg
     8221 tcgcggaacc gccggcgcgc tcgcgcctgg tcgacgaact gcgtcggcag gggatcaacg
     8281 acgaaaaggt gctggcggcg atcgccaggg tgccgcggca tgaattcgta gacggcgcgc
     8341 tgcagtcgcg cgcctacgag aacaacgcgc tgccgatcgg ccacgcgcag accatctcgc
     8401 agccgttcgt ggtggcgctg atgacgcagg cgctcacgca cggccggcgc ctgcgccgcg
     8461 tgctggagat cggcaccggc agcggctacc agaccgcggt gctggccggg ctggtcgaca
     8521 ccgttttcac ggtggagcgg atcaagccgt tgaccgagat cgcgcgcaag cgcctggccg
     8581 cgcagggatg caagaacgtg cacttcggct atgccgatgg cagcaagggc tggtctgcgt
     8641 acgctccgta cgacggcatc atggtcacgg ccgccgccga cagcgtgccg gagg
//
LOCUS       DASZZC010000311         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_86948,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000311 DASZZC010000000
VERSION     DASZZC010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86948"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1235
                     /gene="lptF"
                     /locus_tag="VHE37_06510"
     CDS             135..1235
                     /gene="lptF"
                     /locus_tag="VHE37_06510"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HVT35214.1"
                     /translation="MNKGILDRYLLREVTGAMSAVILVLMAIMLSTRFARFLAQAAAG
                     KLPREVLFKVVALSSLQYLVILIPFSLLLGVMLALGRLYKDNEIAAMTGCGVGMQALY
                     RPFMYVGAVLALVTAVLSFDVGPWAGRTADYLAKDAARYVQFNPFEPGRFKDVAGGRA
                     VFYTAGMDASGEHLEGVFAQIHESAGDSIVLARSGHQQFDESGERKIVLDDGYRYLGN
                     AGNAGYEVIHYGELETRVVPPEFIYSNSKRAIAETATLLASDGLEDRAELQWRLAAPL
                     SVFILGLLAVPLAHVGPRQGRYGKLVLGIGVYLLYSQLLGLGQTWIAQGKVSSLVGLW
                     WVHLLMLAFAGVVIARRQGWFAAWAARRQAAT"
     gene            1232..>2201
                     /gene="lptG"
                     /locus_tag="VHE37_06515"
     CDS             1232..>2201
                     /gene="lptG"
                     /locus_tag="VHE37_06515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HVT35215.1"
                     /translation="MKKIDRYIAQNIYGLTGIVALALVAIYTFVSFVADIDQTGQGSF
                     GLGKLLTYSLMNMPAALYTLFPIIAMLGTLMGLGSLASQGELTAMRAAGVSLLRIGTA
                     TVYAGILLALISLVLGDWLAPLGVRYAEAYRSAARYGVQPGLAGKPVWLREGDHVFHI
                     QRLLAEDHIADVEVYTLRPDFSLAAAMRVDDARYTGSGWTFSGVHTTEFDAQGAHATQ
                     QAQMEWRGNLSPEVLHLFVLESDTLSAPGLARLIAYLDQNHLDASAYRLALWRKLVAP
                     LTVMAMMLFAVPFVIGPLRNTGAGQRLLFGVLIGVAFYVLNEVTASL"
BASE COUNT          315 a          732 c          764 g          390 t
ORIGIN      
        1 cctcgctgcc ttcgtcgtcg ccgctgggct ccccaattct ggtttttgcg gctgctgctt
       61 gtgggcggcg cgcattctag caagaatggc ttttcgccag ccgtttggcg gttcgtgcaa
      121 atcagcccgc aaaaatgaac aaaggcatcc tcgaccgcta cctgctgcgg gaagtcaccg
      181 gcgccatgag cgcggtgatc ctggtgctga tggcgatcat gctgtcgacc cgcttcgcgc
      241 gcttcctggc gcaggcggcg gcgggcaagc tgccgcgcga ggtgctgttc aaggtggtgg
      301 cgctgtccag cctgcagtat ctggtgatcc tgatcccgtt ctcgctgctg ctgggcgtga
      361 tgctggcgct gggccgactg tacaaggaca acgagatcgc cgccatgacc ggctgcggcg
      421 tgggcatgca ggcgctgtac cgcccgttca tgtacgtggg cgcggtgctg gcgctggtca
      481 ccgcggtgct gtcgttcgac gtcggcccgt gggccggccg caccgccgac tacctggcca
      541 aggacgcggc gcgctacgtg cagttcaacc cgttcgagcc gggccgcttc aaggacgtgg
      601 ccggcggccg cgcggtgttc tacaccgccg gcatggacgc cagcggcgag catctggaag
      661 gcgtgttcgc gcagatccac gagagcgccg gcgacagcat cgtgctggcg cgcagcgggc
      721 accagcagtt cgacgagtcc ggcgagcgca agatcgtgct ggatgacggc taccgctacc
      781 ttggcaacgc cggcaatgcc ggttacgagg tgatccacta cggcgaactg gaaacacgcg
      841 tggtgccgcc ggagttcatc tacagcaaca gcaagcgcgc catcgccgag accgccacgc
      901 tgctggcttc cgacgggctg gaagaccgcg ccgagctgca atggcgcctg gccgcgccgc
      961 tgtcggtgtt cattctcggc cttctggcgg tgccgctggc gcatgtcggt ccgcgccagg
     1021 gccgctacgg caagctggtg ctcggcatcg gcgtctacct gctgtactcg cagctgctcg
     1081 gcctgggtca gacctggatt gcgcagggca aggtgtcgtc gctggtgggg ctgtggtggg
     1141 tgcacctgct gatgctggcg ttcgccggcg tggtcatcgc gcggcggcag ggctggttcg
     1201 cggcatgggc ggcgcggcgc caggcggcca cgtgaaaaag atcgaccgct atatcgcgca
     1261 gaacatctac gggctcaccg gcatcgtggc gctggcactg gtcgccatct acaccttcgt
     1321 cagcttcgtc gccgacatcg accagaccgg gcagggcagc ttcggcctgg gcaagctgct
     1381 gacctacagc ctgatgaaca tgccggccgc gctgtacacg ctgttcccca tcatcgccat
     1441 gctcggcacg ctgatgggcc tgggcagcct ggccagccag ggtgaactga ccgcgatgcg
     1501 cgcggccggc gtgtcgctgc tgcgcatcgg cactgcaacg gtctacgccg gcatcctgct
     1561 ggcgctgatc agcctggtgc tgggcgactg gctggcgccg ctgggcgtgc gctacgccga
     1621 ggcctaccgc agtgccgcgc gctacggcgt gcagcccggc ctcgccggca agccggtgtg
     1681 gctgcgcgag ggcgaccacg tgttccacat ccagcgcctg ctggcggaag accatatcgc
     1741 cgatgtcgag gtgtacacgc tcaggcccga cttcagcctg gccgcggcca tgcgcgtgga
     1801 tgacgcgcgc tacaccggca gcggctggac cttttccggc gtgcacacca ctgagttcga
     1861 tgcacaaggc gcgcatgcca cgcaacaggc gcagatggaa tggcgcggca acctgtcgcc
     1921 ggaagtgctg cacctgttcg tgctggagtc cgacacgctg tcggcgccgg gcctggcgcg
     1981 gctgatcgcc tatctcgacc agaaccatct cgacgcctcg gcctaccgcc tggcgctgtg
     2041 gcgcaagctg gtcgcgccgc tgacggtgat ggcgatgatg ctgttcgcgg tgccgttcgt
     2101 catcggcccg ctgcgcaaca ccggcgccgg ccagcgcctg ctgttcggcg tgctgatcgg
     2161 cgtggccttc tacgtgctca acgaagtcac cgcgagcctc g
//
LOCUS       DASZZC010000312         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_474067,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000312 DASZZC010000000
VERSION     DASZZC010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474067"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="VHE37_06520"
     CDS             <1..1862
                     /locus_tag="VHE37_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT35216.1"
                     /translation="TTQSGPLTAVVETTAQYLDPNSNTVVAVPRKLVLRAPVDALASS
                     PVIVSALSTEVLRTMQADALKYAEARQAIAARLSLANTPALPGDLTVTPAQVLSDLSM
                     LDDAGARGALLFEEKALQNRAALAADVLGRGYATQLAVDPVKKVQDAQAIAWAPEDIP
                     RYDHLFVIIFENHPNTVIDLPVFPDFYKYLNTEGNKAANYFSMGNPSEPNYVTLASGD
                     DWGITNDSGWNCVPAGDTADLPTDVYNPRGTCTDDAVHNQKNRRNLFTSLYQAGLSAR
                     VYSESMDPGQDPRVDGLGNPDISGANKRTGQQEPFIADLYKTKHHPAMYFDEVRTRPD
                     FFRNLNRTVGGGQWDDGIAAYAAANHISWNTHQLEDDLKSGDVGALNFIVPDQCDDIH
                     TTGTQVSDCTEVAYVSTGILRGDAYAKYLVDTIEASPLWQNSARRVGIVLVFDEGSFF
                     TGSSSCCGWNVGGGDTAGRPIGEPNDAPTAAYADGDKGDGPTIFGVLNNQAAAPRGVK
                     DSDSYSHISFVRTLQDMYGLADPGVDDSYMNRSKYSESYIAAHLAQLTEYADSADPHF
                     DAVRPMNHAYVMKAGDRESGGLVPGTSGGGSTGTGDFFALGPDPNQINIWALK"
BASE COUNT          349 a          700 c          640 g          276 t
ORIGIN      
        1 gcaccacgca gagcggtccg ctcaccgcgg tggtcgaaac caccgcgcag tacctcgacc
       61 cgaacagcaa caccgtggtc gccgtgccgc gcaagctggt gctgcgtgcg ccggtggacg
      121 cgctcgcgtc ctcgccggtg atcgtcagcg cgctgtcgac cgaggtgctg cgcaccatgc
      181 aggccgatgc gctcaagtac gccgaggcgc ggcaggccat cgcggcgcgg ttgtcgctgg
      241 ccaacacgcc ggcgctgccg ggcgacctga cagtgacgcc ggcgcaggtg ctgtccgacc
      301 tgtccatgct ggacgacgcc ggcgcgcgcg gcgcgctgct gttcgaggag aaagcgctgc
      361 agaaccgcgc ggcgctggcc gccgatgtgc tcggccgcgg ctacgccacg cagctggcgg
      421 tcgacccggt gaagaaggtg caggacgcgc aggccatcgc ctgggcgccg gaagacatcc
      481 cgcgctacga ccacctgttc gtgatcatct tcgagaacca tccgaacacg gtgatcgacc
      541 tgccggtgtt ccccgacttc tacaagtacc tcaacaccga gggcaacaag gcggccaact
      601 atttctccat gggcaacccg agcgagccga actacgtgac gctggccagc ggcgatgact
      661 ggggcatcac taatgacagc ggctggaact gcgtgcctgc tggcgacacc gccgacctgc
      721 cgaccgacgt ctacaacccg cgcggcacct gcaccgacga cgccgtgcat aaccagaaga
      781 accgccgcaa cctgttcacc tcgctctacc aggccggcct ttccgcgcgc gtgtacagcg
      841 aatcgatgga cccgggccag gacccgcgcg tggatggcct gggcaacccg gacatcagcg
      901 gcgccaacaa gcgcaccggc cagcaggagc cgttcatcgc cgacctgtac aagaccaagc
      961 atcacccggc gatgtacttc gacgaggtgc gcacgcggcc cgatttcttc cgcaacctca
     1021 accgcaccgt cggcggcggc cagtgggacg acggcatcgc cgcgtatgcc gcggccaacc
     1081 acatcagctg gaacacgcac cagctggaag acgacctcaa gtcgggcgac gtcggcgcgc
     1141 tgaacttcat cgtgccggac cagtgcgatg acatccacac caccggcacg caggtgtcgg
     1201 actgcaccga ggtggcctac gtctccaccg gcatcctgcg cggcgacgcg tatgccaagt
     1261 acctggtcga taccatcgag gcctcgccgc tgtggcagaa cagcgcgcgc cgcgtcggca
     1321 tcgtgctggt gttcgacgaa ggctcgttct tcaccggcag ctcctcctgc tgcggctgga
     1381 acgtcggcgg cggcgatacc gccggccggc cgatcggcga gccgaacgat gcgccgaccg
     1441 cggcctatgc cgacggcgac aagggcgacg gcccgaccat cttcggcgtg ctcaacaacc
     1501 aggcggcggc gcccaggggc gtgaaggatt ccgacagcta cagccacatc tccttcgtgc
     1561 gcacgctgca ggacatgtac ggcctggccg atccgggcgt cgacgattcg tacatgaacc
     1621 gctccaagta cagcgagagc tacatcgccg cgcacctggc ccagctgacc gagtatgccg
     1681 acagcgccga tccgcatttc gacgcggtgc ggccgatgaa ccatgcctac gtgatgaagg
     1741 ccggcgaccg cgagtccggc ggcctggtgc ctggcaccag cggcggcggc agcaccggca
     1801 ccggcgactt cttcgcgctg gggcccgacc cgaaccagat caacatctgg gcgctgaagt
     1861 agcggcatgc gccgtgttgc gcttgctacg gttttcgtag cggcgctggc gccgacggcg
     1921 caggcgcagc tgagcccgcg cgcgcagctc ggccggctga tgttc
//
LOCUS       DASZZC010000313         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_989935,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000313 DASZZC010000000
VERSION     DASZZC010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989935"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VHE37_06525"
     CDS             <1..212
                     /locus_tag="VHE37_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35217.1"
                     /translation="QLTARGIANVRTASYSLAAFQPSPKMDAALKKLNVVMTGEIPIA
                     LVNGMGKANPTLEDVVSEFNKTSAK"
     gene            complement(369..716)
                     /locus_tag="VHE37_06530"
     CDS             complement(369..716)
                     /locus_tag="VHE37_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HVT35218.1"
                     /translation="MNWSVLGQAGGLLVLSNVFMTFAWYAHLKDLRDKPWIVAALVSW
                     GIALFEYLLQVPANRIGYSALTLPQLKIMQEVITLAVFVPFALYFMKTPLKLDYLWAA
                     LCMCGAVYFIFRS"
     gene            806..1240
                     /locus_tag="VHE37_06535"
     CDS             806..1240
                     /locus_tag="VHE37_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVT35219.1"
                     /translation="MNVTQYQPWSLHRDLLNEFNRYFERASGDASSGATADWAPAVDI
                     EEYADKFVLYADVPGVDPASIELTLENGVLSVTGSRERRVEQAGVEKSRVERATGRFF
                     RRFALPDTVDSDSVSASNKNGVLEIVIPKRAQAQARKIAVKH"
     gene            1416..2294
                     /locus_tag="VHE37_06540"
     CDS             1416..2294
                     /locus_tag="VHE37_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HVT35220.1"
                     /translation="MEYKDYYKVLGVSRTASEDEIKRAYRKLARQYHPDKNKAKGAEE
                     KFKEINEANEVLSDPKKRQAYDSLGANWKAGSQFTPPPGWQNFGAGRGRVGGMDFSDF
                     FSSLFGGGMGGVGGGGFEDMGGGFGGGQDARARIAITLEDSYHGAQKQISLSTGRTLN
                     VRIPKGITAGQTIRLAQQGPHGGDLLLEVEFAPHAQFKVDGRDVSSTVKIAPWEAALG
                     GKVPVVTLGGTVELNVPAGSGTGKKMRLKGRGLPGTTPGDQLVKLQVVTPPAANAGDE
                     EFYRKMAEHFRFDPRA"
     gene            2328..2525
                     /locus_tag="VHE37_06545"
     CDS             2328..2525
                     /locus_tag="VHE37_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35221.1"
                     /translation="MRQEQEVLPAPLTPDVQQLRNSLGPLAEFRYAEIASAGIARAAA
                     ARWPRLAEQLRAAAKERHGPA"
     gene            2512..>3379
                     /locus_tag="VHE37_06550"
     CDS             2512..>3379
                     /locus_tag="VHE37_06550"
                     /inference="COORDINATES: protein motif:HMM:NF022448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BcsE family c-di-GMP-binding protein"
                     /protein_id="HVT35222.1"
                     /translation="MARRDPFPVSLGIEGLPPASAQMEPDAAYAVGVPLPHALPALVC
                     GMAARALLDRRPVTLVCGSDTAEIVRRLTAHGLDAPAEMAAGRLVVLQLSDSLDTKLG
                     HFGASRVIEELEYFCKPRGRLLILAPAEQLLVANNPRLAARQAELYARWCTASRAAAV
                     FVFSGNSVASACARQAVDGWFAGVARMSGDGLRLNWQVEHWRSPEGSIGSREYGISVA
                     HDGALLVADGGLRSSSLKIVEAPDYDTVLAVAAAVRHEKSRPAHWQLVGGHEALLTRA
                     SSAVAATFILDCG"
BASE COUNT          604 a         1155 c         1123 g          497 t
ORIGIN      
        1 ggcaactgac cgcgcggggc atcgccaatg tgcggaccgc cagctactcg ctggcggcgt
       61 tccagccgtc accaaagatg gatgccgcac tgaagaagct gaacgtcgtc atgaccggcg
      121 agatcccgat cgcgctggtc aatggcatgg gcaaggccaa tcccacgctg gaagacgtgg
      181 tgtctgaatt caacaagacg tccgcaaaat gaaggcggcg ctcaaccgtt gcggaagatg
      241 aagcagaccg cgccgcacat gcacagagcg gcccacaggc agcgacccga gttccgcgac
      301 ccgtgcgcag cacgcgatcg cgcgccgttg gcgagtggtg gcctatttgt gggccttccc
      361 ggccacgatc acgaacggaa gatgaagtag accgccccgc acatgcacag agccgcccac
      421 agatagtcga gcttcagcgg cgtcttcatg aagtacagcg caaacggcac gaacaccgcg
      481 agcgtgatca cctcctgcat gatcttgagc tgcggcaggg tcagcgcgct gtagccgatg
      541 cggttggccg gcacctgcag caggtactcg aacagggcga tgccccagct gaccagcgca
      601 gccacgatcc acggcttgtc gcgcaggtcc ttcaggtgcg cgtaccaggc aaacgtcatg
      661 aagacgttcg acaggaccag caggccgccg gcctgtccca gcacactcca attcatcctt
      721 ggcacccctt gaaaacctgc tgacccgccc aattctaggt ggcgtccggg ccgccgtgcc
      781 cggttcccaa cacggagacc atattatgaa cgtgacccag taccagccct ggtccctgca
      841 ccgggacctg cttaacgagt tcaaccgcta cttcgagcgc gcctccggcg acgccagttc
      901 cggcgccacc gccgactggg cgccggcggt cgacatcgag gagtacgccg acaagttcgt
      961 gctctacgcc gatgtgcccg gggtcgatcc cgccagcatc gaactgacgc tggaaaacgg
     1021 cgtgctcagc gtcaccggct cgcgcgaacg ccgcgtcgag caggccggcg tcgagaagag
     1081 ccgcgtcgag cgcgccaccg gccgcttctt ccgccgcttc gccctgcccg acacggtgga
     1141 ttccgattcg gtatcggcat cgaacaagaa cggcgtgctc gagatcgtga ttcccaagcg
     1201 cgcacaggcg caggcgcgca agatcgcggt caagcactga aacacggtgt tgtcattccc
     1261 gcgcaagcgg gaatctgtcg gcacctcgct caagggcatc cggtaaaaaa ctggatgccc
     1321 gccttcgcgg gcatgacgga gaatttcagg gggcgcacgt tgcgcccctt ttttgttcta
     1381 atcggatcaa agcaaagcga tcactgactc gaacgatgga atacaaggac tactacaagg
     1441 tactcggcgt ctcccgcacc gccagcgagg acgagatcaa gcgcgcctac cgcaagctgg
     1501 cgcgccagta ccatccggac aagaacaagg ccaagggcgc ggaagagaag ttcaaggaga
     1561 tcaacgaggc caacgaggtc ctgtcggacc cgaagaagcg ccaggcctac gactcgctgg
     1621 gcgccaactg gaaggccggc tcgcagttca cgccgccgcc gggctggcag aacttcggcg
     1681 ctggccgcgg ccgcgtcggc ggcatggact tctccgattt tttctcctcg ctgttcggcg
     1741 gcggcatggg cggggttggg ggcggcggct tcgaggacat gggcggcggt ttcggcggcg
     1801 ggcaggatgc gcgcgcgcgt atcgccatca cgctggagga ttcgtaccac ggcgcgcaga
     1861 agcagatctc gctgtccacc ggacgcacgc tcaacgtgcg catccccaag ggcatcaccg
     1921 ccggccagac catccgactc gcacagcagg ggccgcacgg cggcgacctg ctgctggaag
     1981 tggaatttgc accgcatgcg cagttcaagg tcgacggccg cgacgtctcc tccacggtga
     2041 agattgcgcc gtgggaagcc gcgctcggcg gcaaggtgcc ggtggtaacg ctcggcggca
     2101 cggtggaact gaacgtgccg gccggctcgg gcaccggcaa gaagatgcgc ctgaagggcc
     2161 gcggcctgcc cggtaccacg cccggcgacc agctggtcaa attgcaggtg gtaacgccgc
     2221 ccgccgcgaa tgccggcgac gaggagttct accgcaagat ggccgagcat ttccgcttcg
     2281 atccgcgcgc ctgaccgcaa cgcgcgcggg acgctagaat cccgcgcatg cgtcaagaac
     2341 aagaagtgct gccggcaccg ctgacgccgg acgtgcaaca gctgcggaat tccctcggcc
     2401 cgctggcgga gttccgctac gccgagatcg cctcggccgg catcgcccgc gccgccgccg
     2461 cccgctggcc gcgccttgcc gagcagctgc gcgctgccgc gaaggagcgc catggcccgg
     2521 cgtgacccgt ttccggtttc cctcggcatc gaagggctgc cgcccgcctc cgcccagatg
     2581 gaacccgatg cggcctacgc cgtcggcgtg ccgctgccgc atgcgctgcc cgcgctggtc
     2641 tgtggcatgg cggcgcgcgc actgcttgac cgccggccgg tcacgctggt gtgcggcagc
     2701 gacaccgcgg aaatcgtgcg gcggctgacg gcccacggcc tcgacgcgcc ggcggagatg
     2761 gccgcgggcc gcctggtcgt gctgcagctg tcggacagcc tcgacaccaa gctcggccac
     2821 ttcggcgcca gccgcgtgat cgaggagctg gaatatttct gcaagccgcg cgggcggctg
     2881 ttaatcctgg cgccggcgga acaattgctg gtcgccaaca acccgcgcct ggccgcgcgg
     2941 caggcggaac tctacgcgcg ctggtgcacg gccagccgcg ccgccgcggt gttcgtgttc
     3001 agcggcaact cggtggcctc ggcctgcgcg cgccaggcgg tggatggctg gttcgccggc
     3061 gtggctcgca tgagcggcga cggcctgcgc ctcaactggc aggtggaaca ctggcgttcg
     3121 ccggaaggct ccatcggcag ccgcgagtac ggcatcagcg tggcgcacga cggcgcgctg
     3181 ctggtggccg acggcggcct gcggtcgtcc agcctcaaga tcgtcgaagc accggactac
     3241 gacaccgtgc tcgcagtggc cgcggccgtg cgccacgaga aatcgcggcc tgcgcactgg
     3301 cagctggtcg gcgggcacga ggcgctgctc acacgcgcgt caagcgcggt cgccgccacc
     3361 ttcatcctcg actgcggtc
//
LOCUS       DASZZC010000314         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_793773,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000314 DASZZC010000000
VERSION     DASZZC010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793773"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VHE37_06555"
     CDS             complement(<1..705)
                     /locus_tag="VHE37_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HVT35223.1"
                     /translation="MSKPTYSLVNNATGEKLDLTGVSGTLGPVGLDVGKLYDKEGVFT
                     YDPGFTSTCSTKSAITYIDGDAGVLLYRGYPVDQLAEHCSFIEVAYLVLNGELPNKTQ
                     LEEFDRSIRKHTMINESLKGFFNGFHYDAHPMAMLTGIVGSLSAFYHDTTNNKDPRHR
                     EIFAHRMIAKIPTIAAAAYKKYYGQPFMYPQNHLDYCSNLLHMMFAVPAEPYHVDPIA
                     AKALDVLFILHADHEQN"
     gene            complement(733..1989)
                     /locus_tag="VHE37_06560"
     CDS             complement(733..1989)
                     /locus_tag="VHE37_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HVT35224.1"
                     /translation="MNDTLKKSALDYHEFPRPGKLAIEISKPVNGPGDLSLAYTPGVA
                     EPVLAIQAHPDDAYRYTAKGNLVAVISNGTAVLGLGDCGALAGKPVMEGKAVLFKKFA
                     DVDVFDIEVDAREPKDFIDTVARIAPTFGGINLEDIAAPHCFEIEDKLVERLDIPVFH
                     DDQHGTAIVLCAGLLNALELQGKKIENARIVCLGAGAAGIASMRLALSLGAKRENMLL
                     VDRKGVIHRDREDFGTLNKEKLEFASSAAAERRTLAGALAGADVFIGVSGPGLMKPEL
                     IMLMAEKPICFALSNPVPEIMPDAALSVRNDLIMATGRSDFPNQVNNVLCFPFLFRGA
                     LDVRARTINAPMKVAAVHALASLAREPVPHEVLAAYGRKSLSFGPEYIIPKPLDPRLR
                     NAVAPAVARAAVESGAARMPYPKHYP"
BASE COUNT          341 a          664 c          729 g          412 t
ORIGIN      
        1 gttctgctcg tggtcggcgt gcaggatgaa cagcacgtcc agcgccttgg ccgcgatcgg
       61 atcgacgtgg tacggctcgg ccggcaccgc gaacatcatg tgcagcaggt tgctgcagta
      121 gtcgaggtgg ttctgcgggt acatgaacgg ctggccgtag tacttcttgt acgccgccgc
      181 ggcgatggtc gggatcttgg cgatcatgcg gtgcgcgaag atttcgcggt gccgcggatc
      241 cttgttgttg gtggtgtcgt ggtagaaggc cgacagcgag ccgacgatgc cggtgagcat
      301 ggccatcggg tgcgcgtcgt agtggaatcc gttgaagaag cccttcaacg actcgttgat
      361 catggtgtgc ttgcggatgc tgcggtcgaa ctcttccagc tgcgtcttgt tcggcagctc
      421 gccgttgagc accaggtagg cgacctcgat gaagctgcaa tgctccgcca gctggtccac
      481 cgggtagccg cggtacagca gcacgccggc gtcgccgtcg atgtaggtaa tggcggactt
      541 ggtcgagcag gtcgaggtga agcccgggtc gtaggtgaac acgccttcct tgtcgtacag
      601 cttgcccaca tccaggccca ccgggccgag cgtgccggac acgccggtga ggtcgagctt
      661 ctcgccggtg gcgttgttga cgaggctgta ggtgggcttg ctcatgactg cttcctttga
      721 acctgcgaac ggctagggat agtgcttggg atacggcatg cgagcggcgc cactctccac
      781 cgctgcgcgc gccacggccg gcgcaacggc gttgcgcagg cggggatcga gcggcttggg
      841 aatgatgtac tcggggccga aggatagcga ttttctgccg tacgccgcca gcacttcatg
      901 cggcaccggc tcccgcgcca gcgacgccag cgcatgcacc gccgcgacct tcatcggtgc
      961 attgatggtg cgcgcgcgca catccagtgc accgcggaac aggaacggga agcacagcac
     1021 gttgttcacc tggttgggga agtcgctgcg cccggtggcc atgatcaggt cgttgcgcac
     1081 cgacagcgcc gcgtccggca tgatctccgg caccgggttg gacagcgcga agcagatcgg
     1141 cttctccgcc atcagcatga tcagttccgg cttcatcagt cccggcccgg acacgccgat
     1201 gaacacgtcg gcgccggcca gcgcaccggc cagcgtgcgc cgctcggccg cggcagaaga
     1261 ggcaaactcc agcttctcct tgttcagcgt gccgaagtct tcgcggtcgc ggtggatcac
     1321 gcccttgcgg tccaccagca gcatgttctc gcgcttcgcg ccgagcgaca gcgccaggcg
     1381 catcgacgcg atgccggccg caccggcgcc caggcagacg atgcgcgcgt tctcgatctt
     1441 cttgccctgc agttccagcg cgttgagcag gccggcacac agcacgatgg cggtgccgtg
     1501 ctggtcgtcg tggaataccg ggatgtcgag gcgttcgacc agtttgtcct cgatctcgaa
     1561 gcaatgcggc gcggcgatgt cttccaggtt gatgccgccg aaggtcggcg cgatgcgcgc
     1621 caccgtgtcg atgaagtcct tgggctcgcg cgcgtcgacc tcgatgtcga acacgtcgac
     1681 gtcggcgaac ttcttgaaca gcaccgcctt gccttccatg accggcttgc cggccagcgc
     1741 cccgcagtcg cccaggccga gcaccgcggt gccgttgctg atcactgcaa cgagattgcc
     1801 cttggcggtg tagcggtagg catcgtcggg atgcgcctgg atcgccagca ccggctcggc
     1861 gacgccgggg gtgtaggcca gcgacaggtc gccggggccg ttcaccggct tggagatttc
     1921 aatggcgagc tttccggggc gcggaaactc gtggtagtcc agcgcggact tcttcagggt
     1981 gtcgttcatg cgcagtgcct tcttcctgga tacgcgatgc gggtgacgca aaaaacaagg
     2041 ccgcgcatcg cgcggccttg ggtgtcgatg attgcttgaa aaaactcagg ccgccgcctt
     2101 cttggcgtaa cgcttgttga acttgtctac tcggccgccg gtgtcg
//
LOCUS       DASZZC010000315         5996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_402476,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000315 DASZZC010000000
VERSION     DASZZC010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402476"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VHE37_06565"
     CDS             complement(<1..656)
                     /locus_tag="VHE37_06565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT35225.1"
                     /translation="MVSANVQNVIDHYTRRHAFRQDLFGSQPYANYGYWTRPGQTIEE
                     AAEALTDLVARTAGIGHGDRVLDVGCGYGAGAVSYTQRYQPAYVVGIDITDIRIESGR
                     EYVAEHHLSDVISLRLGDATHMDFADASFTRLIAVECAFHFETRRDFMHEAARVLAPG
                     GRLALTDIIPRRGVDPRTYLQGERTANSNVCLDMPANAYDADTYARYLAEAGFSEIRV
                     "
     gene            complement(772..1992)
                     /locus_tag="VHE37_06570"
     CDS             complement(772..1992)
                     /locus_tag="VHE37_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT35226.1"
                     /translation="MNLLATLRVAARALLRNKLRSFLTALGIIIGVAAVIAMVSIGEG
                     AKARVQEAFASMGTNLLIVLSGSTTSGGARGGFGSQPTLTWDDLKAIQTEVPSVVRAA
                     PQLRSAGPVLSDDQNWTTSITGTTADYFSIRAWRVSSGEDMNDSDNDSRSKRIWLGQT
                     VVDKLYGPNVDPTGLVVRVRNIPFEVAGVLERKGQTSTGQDFDDAVFVPANTFGVLIQ
                     GGLKKYLSGAIFVQASDTQRAEEQITALLRDRHRLRAGTDDDFSIRNLAEMASAQEEG
                     TNTMTTLLASVAAVSLFVGGIGIMNIMLVSVTERTREIGTRMAVGARPRDILLQFLIE
                     ALVLSLLGGLLGIALGLGVAAQLAGKFGWPLLIRSDIVLVSVLVSAGVGVVFGLYPAR
                     KASLLDPIEALRYE"
     gene            complement(1989..2696)
                     /locus_tag="VHE37_06575"
     CDS             complement(1989..2696)
                     /locus_tag="VHE37_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT35227.1"
                     /translation="MALIELAEVRKIYRMGETDVAALDGVTLAIERGDFVAVMGSSGS
                     GKSTLMNILGCLDRPSSGEYRLDGTLVAEQNRDELAHIRNRTLGFVFQNFNLLTRTSA
                     LENVELPLLYAGVNAAERHRRARAALERVGLGARVDHHPNQLSGGQQQRVAIARALVS
                     EPKLILADEPTGNLDTRTSEEVMALLQQLGAQGITVVLVTHEADIAAHAARIVTMRDG
                     RVLTDERRAPLPAGAPA"
     gene            complement(2696..3907)
                     /locus_tag="VHE37_06580"
     CDS             complement(2696..3907)
                     /locus_tag="VHE37_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT35228.1"
                     /translation="MNRKTVVTTLACAIVAALLFMLWRSGSHKAAPDYVTVKADRGRI
                     AARVTATGTLSALVTVQVGSQVSGRIADIEVDYNSTVKKGQLLARIDPQLFEAALQQA
                     QANLKAAQGNLVKARVQAKDAHRQADRSDELYAQKLIAQSDRDTARANADAADAAIVA
                     ADGEVAQAQASADQASVNLAYTKILSPTDGVVISRAVDVGQTVAAALQAPTLFVIAQD
                     LSQMQVDTSVAESDIGKLTEGMPVMFTVDAYPNERFRGSVRQIRNAPTTVQNVVTYDA
                     VIAVANPDLKLKPGMTANATFTYADKSDVLRVPNAALRFKPAADQVPGPKADAPREHG
                     QRGGGDRSQRTLWVLRGGQAQPVTVQTGISDGSLTEIADGDLHEGDDLITDIKGGTAS
                     KPASAPGPRMF"
     gene            4058..4672
                     /locus_tag="VHE37_06585"
     CDS             4058..4672
                     /locus_tag="VHE37_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVT35229.1"
                     /translation="MRHLIWTLLAAALLAAAGSRAAAPDEPADYKGMLEAHDTWRRPV
                     GSPDLLWSHEAAQQAQAWADELARNECQVHHSSEEQRRGDFGENIYYYWSSRPYEGYR
                     RDPATVVSSWGKEIEFYDEQNDECHAPAGRTCRHYTQVVWSRTTHVGCGRARCDAAEV
                     WVCEYSPRGNIYPVRPYEPRGSSASGAESRELRPQQAAPAAPPP"
     gene            4791..5165
                     /locus_tag="VHE37_06590"
     CDS             4791..5165
                     /locus_tag="VHE37_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35230.1"
                     /translation="MRVTNEVIQNAVLQIFMDQKIPEGGCLRPHQLRGHWPHTRLRQG
                     DLVQGLKQLVFNGYLELDDDYQGGMFILTAVGYQRALKLPSGPRGTWNQFVASALLNV
                     ARRRPTAEHNDGRRATDVVAAS"
     gene            complement(5255..>5996)
                     /locus_tag="VHE37_06595"
     CDS             complement(5255..>5996)
                     /locus_tag="VHE37_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT35231.1"
                     /translation="VGTLADWRAAYLAGGGAGLLVLGGRLLVLEESALFEQSGAAARG
                     QLALLFGRGELLRRFLAVIAVGVPIWYVSALFVTLAPEYGRALGFADPLTVAQVLRWQ
                     AVGIALGSACTGVASEWLRSRRRVLYGCIGALAGMTALLLHAASPDRYIVLMFFIGLA
                     QGYWTAFITMATEQFGTNLRGTVASAAPNIVRAASVPVTLGAQALMPVLGLLQATLLI
                     GVVVYALALFGLARLRETWGSDLAFSEG"
BASE COUNT          939 a         2093 c         2015 g          949 t
ORIGIN      
        1 tcgacgcgga tctcgctgaa accggcctcg gccaggtagc gcgcgtaggt gtcggcgtcg
       61 taggcgttgg ccggcatgtc caggcagacg ttgctgttgg cggtgcgctc gccctgcagg
      121 taagtgcgcg gatcgacgcc acgccgcggg atgatgtcgg tgagcgccag ccgtccgccc
      181 ggcgccagca cgcgtgccgc ttcatgcatg aaatcgcgcc gcgtttcaaa gtggaacgcg
      241 cactccaccg cgatcaggcg ggtgaagctg gcatcggcga aatccatgtg cgtggcatcg
      301 cccaggcgca gcgagatgac gtccgacaga tgatgttccg ccacgtattc gcggccgctc
      361 tcgatgcgga tatcggtgat gtcgatgccg accacatagg ccggctggta gcgctgcgtg
      421 tacgacaccg cgccggcgcc gtagccgcaa ccgacgtcga gcacgcggtc accgtggccg
      481 atgccggcgg tgcgtgccac caggtcggtc agcgcctccg ccgcttcctc gatggtctgc
      541 ccgggccgtg tccagtagcc gtagttggcg tagggctggc tgccgaacag gtcctgccgg
      601 aaggcatgcc ggcgggtgta gtgatcgatg acgttctgga cgttggcgga gaccatgggg
      661 agccctccgt ggcgggagtg ttgatacgac cgtgagtgtt gcatgcgggt gccgcagcat
      721 gtcccgcaat cgactgtcag ccgagactta cgcaagctga gagtgccgtc actattcgta
      781 gcgcagcgcc tcgatcggat caagcagcga ggccttgcgt gcgggataca ggccgaacac
      841 cacgcccacg ccggccgaca ccagcaccga caccagcacg atgtcgctgc ggatcagcag
      901 cggccagccg aacttgccgg cgagttgggc cgccacgccg agcccaagcg cgatgcccag
      961 caggccgcca agcagcgaca gcaccagcgc ctcgatcagg aactgcagca ggatgtcgcg
     1021 cggccgcgcg cccaccgcca tgcgcgtgcc gatctcgcgc gtgcgctcgg tcaccgacac
     1081 cagcatgatg ttcatgatgc cgatgccgcc gacgaacagc gacacggcgg ccacgctggc
     1141 cagcagcgtg gtcatggtat tggtgccctc ttcctgcgcg ctggccatct cggccaggtt
     1201 gcggatggag aaatcgtcgt cggtacccgc acgcaggcgg tggcggtcgc gcagcagcgc
     1261 ggtgatctgc tcctccgcgc gctgcgtgtc ggacgcctgc acgaagatgg cgccgctcag
     1321 gtatttcttc aaccctccct ggatcagcac gccgaaggtg tttgccggca cgaacaccgc
     1381 gtcgtcgaaa tcctggccgg tgctggtctg gcccttgcgc tcgagcacgc cggccacctc
     1441 gaacgggatg ttgcgcacgc gcaccaccag cccggtggga tcgacgttgg gaccgtagag
     1501 cttgtccacc accgtctgcc ccagccagat gcgcttgctg cggctgtcgt tgtcggagtc
     1561 gttcatgtcc tcgccggagg acacgcgcca cgcgcggatg gagaaataat ccgccgtggt
     1621 gccggtgatc gacgtggtcc agttctggtc gtcggacagc accggcccgg cgctgcgcag
     1681 ctgcggcgcc gcgcgcacca cgctgggcac ctcggtctgg atcgccttga gatcatccca
     1741 ggtcagcgtg ggctgcgagc cgaaaccgcc gcgcgcgccg ccggaggtgg tggagcccga
     1801 cagcacgatc agcaggttgg tgcccatgga ggcgaacgct tcctgcacgc gcgccttggc
     1861 gccctcgccg atcgacacca tggcgatcac cgcggccacg ccgatgatga tgcccagcgc
     1921 ggtgaggaac gagcgcagct tgttgcgcag cagggcgcgc gccgccacgc gcaaggtcgc
     1981 cagcaggttc acgccggcgc tcccgccggc agcggcgcac ggcgctcgtc ggtcagcacg
     2041 cggccgtcgc gcatggtgac gatgcgcgcg gcatgcgccg cgatgtcggc ctcgtgcgtc
     2101 accagcacca cggtgatgcc ttgcgcgccg agctgctgca gcagcgccat cacttcctcg
     2161 ctggtgcgcg tgtcgaggtt gccggtgggc tcgtcggcca ggatcagctt gggctcggac
     2221 accagcgccc gtgcgatggc cacgcgttgc tgctggccgc cggacagctg gttgggatga
     2281 tgatcgacgc gcgcgcccag gcccacgcgc tccagcgcgg cgcgcgcgcg ccggtgccgc
     2341 tcggccgcgt tgacgccggc atacagcagc ggcagctcga cgttttccag cgcgctggtg
     2401 cgcgtcagca ggttgaagtt ctggaacacg aagcccagcg tgcggttgcg gatatgcgcc
     2461 agttcgtcgc ggttctgctc ggccaccagc gtgccgtcga gccggtactc gccggagctc
     2521 gggcggtcga gacaccccag gatgttcatc agcgtcgact tgccgctgcc ggacgagccc
     2581 atcaccgcga cgaaatcgcc gcgctcgatg gccagcgtga cgccgtccag cgcggccacg
     2641 tcggtttcac ccatgcggta gatcttgcgg acctccgcca gctcgatcag cgccatcaga
     2701 acatccgcgg tcccggcgcc gatgccggct tgctcgccgt gccgcccttg atgtcggtga
     2761 tcaggtcgtc gccctcgtgc aggtcgccgt cggcgatctc ggtcagcgag ccgtcggaga
     2821 tgccggtctg caccgtcacc ggctgggcct ggccgccgcg cagcacccac agcgtgcgct
     2881 ggctgcggtc accgccgcct ctctggccgt gctcgcgcgg cgcatcggcc ttcggtcccg
     2941 gcacctggtc tgctgccggc ttgaagcgca gcgcagcgtt gggcacgcgc agcacgtcgg
     3001 acttgtcggc gtaggtgaac gtggcgttgg cggtcatgcc cggcttgagc ttcagatcag
     3061 gattggccac cgcgatcacc gcgtcgtagg tcaccacgtt ctgcaccgtg gtgggcgcgt
     3121 tgcggatctg gcgcacgctg ccgcggaagc gctcgttggg atacgcatcg acggtgaaca
     3181 tcaccggcat gccctcggtc agcttgccga tgtcggactc ggccacgctg gtgtcgacct
     3241 gcatctgcga caggtcctgg gcgatgacga acagcgtcgg cgcctgcagc gccgcagcca
     3301 ccgtctgccc gacatcgacc gcacgcgaga tgaccacgcc gtcggtcggc gacaggatct
     3361 tggtgtacgc gaggttgacc gaggcctggt cggccgaagc ctgcgcctgc gccacttcac
     3421 cgtcggcggc gacgatggcc gcatccgccg cgtcggcatt ggcacgcgcg gtatcgcggt
     3481 cggactgcgc gatgagtttc tgtgcataca gttcgtcgga gcggtcggcc tggcggtgcg
     3541 cgtccttggc ctgcaccctg gccttgacca gattgccctg cgccgccttg aggttggcct
     3601 gcgcctgctg cagcgccgcc tcgaacagct gcggatcgat gcgcgccagc agctgaccct
     3661 tcttcaccgt cgagttgtag tccacttcga tgtcggcgat gcggccggac acctggctgc
     3721 cgacctgcac cgtcaccagc gcagacaggg ttccggtcgc ggtgacacgc gcggcaatgc
     3781 gcccgcgatc ggccttcacc gtcacgtaat cgggcgcagc cttgtggctg cccgaacgcc
     3841 ataacatgaa cagcagcgcc gccacgatgg cgcatgcgag cgtcgttacc acggtcttgc
     3901 ggttcatcgc gctgatttct ttttcggcgg ttaacggcag gccatcctgc ctgtgcgtag
     3961 acagcggcgg caagacttcg acgccggcca gcgggtaaag ctcccttaac catcgcgcgc
     4021 cgaccggcta ggctttgcgc aaatccacgg agtgctcatg cgccacctga tatggacatt
     4081 gctcgccgct gccctgctgg cagcggccgg ttcacgcgcg gcagcgcccg acgagccagc
     4141 tgactacaag ggcatgctcg aagcgcacga cacctggcgc cgcccggtgg gctcgccgga
     4201 tctgctgtgg tcgcacgagg cggcgcagca ggcgcaggcc tgggccgacg agctggcccg
     4261 caacgagtgc caggtgcacc acagcagcga ggagcagcgc cgcggcgatt tcggcgagaa
     4321 catctattac tactggtcct cgcgccctta cgaaggctac cggcgcgatc ccgccacggt
     4381 ggtgtccagc tggggcaagg agatcgagtt ctacgacgag cagaacgacg agtgccatgc
     4441 gccggcggga cgcacctgcc ggcactacac gcaggtggtg tggagccgca ccacccacgt
     4501 cggttgcggg cgcgcacgtt gcgacgcggc ggaagtatgg gtgtgcgaat actcaccgcg
     4561 cggcaacatc tatccggtgc ggccgtacga gccgcgcggc agcagcgcaa gcggagcgga
     4621 aagccgtgag ctccgcccgc agcaggccgc tccggcggcg ccgccaccct gacttatccc
     4681 caggacctgc gacggctgtc attcatcctc cgcaagcgga ccttttgtcg tccgtcacgt
     4741 ttattggcgc cggggggctc taatctggct tccgtcggat cgagggcgtc atgcgcgtca
     4801 ccaacgaagt catccagaac gcagtgctgc agatcttcat ggaccagaag atcccggagg
     4861 gcggctgcct gcgcccgcat cagttgcgcg gacactggcc gcacacccgc ctgcgccagg
     4921 gcgacctggt gcagggtctc aagcagctgg tgttcaacgg ctacctcgaa ctggatgacg
     4981 attatcaggg cggcatgttc atcctcaccg ccgtcggcta ccagcgcgcg ctcaagctgc
     5041 cttccggccc gcgcggcacc tggaaccaat tcgtggcctc ggcgctgctc aacgtggcgc
     5101 gccgccggcc tactgcggaa cacaacgacg gccgccgcgc gaccgacgtg gtggccgcca
     5161 gctagtttct ctcccccgcc ggcaccccca agccggcatt ctcggggcgc gataccttcg
     5221 ggttcgcgcc ctttttttat tcagcctttt tcattcagcc ttcgctgaag gcaaggtcac
     5281 tgccccaggt ctcgcgcagc cgcgccaggc cgaacagggc aagggcgtag accaccacgc
     5341 cgatcagcag cgtcgcctgc agcaggccca gcaccggcat caaggcctgc gcacccagcg
     5401 tgaccggcac gctggcggca cgcacgatgt tcggtgcggc gctggccaca gttccgcgca
     5461 ggttggtgcc gaactgctcg gtggccatgg tgatgaaggc ggtccagtaa ccctgcgcca
     5521 ggccgatgaa gaacatcagc acgatgtagc ggtccggcga cgccgcatgc agcagcagcg
     5581 ccgtcatccc ggccagggcg ccgatgcagc cgtagagcac gcggcggcgc gagcgcagcc
     5641 attcgctggc cacgccggtg cacgcgctgc ccagcgcgat gcccaccgcc tgccagcgca
     5701 gcacctgcgc cacggtcagc ggatcggcaa atcccagcgc acggccgtac tcgggcgcca
     5761 gcgtgacgaa cagcgccgac acgtaccaga tcggcacgcc caccgcgatc accgcgagga
     5821 accggcgcag cagttcgccg cgcccgaaca gcagcgcgag ctggccgcgc gccgcagcgc
     5881 cggattgctc gaacagcgcc gattcttcca gcaccagcag ccgcccgccg agcaccagca
     5941 gcccggcacc gccgccggcg aggtacgcgg ctcgccagtc ggccagcgtg ccgacg
//
LOCUS       DASZZC010000316         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_928010,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000316 DASZZC010000000
VERSION     DASZZC010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928010"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..385
                     /gene="thiS"
                     /locus_tag="VHE37_06600"
     CDS             185..385
                     /gene="thiS"
                     /locus_tag="VHE37_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HVT35232.1"
                     /translation="MNLIVNGEARLLPPGTSVQMLMAELGLAGKRAAVERNGEIVPRS
                     LYAQTTLAEGDRIEIVQAIGGG"
     gene            complement(394..1611)
                     /locus_tag="VHE37_06605"
     CDS             complement(394..1611)
                     /locus_tag="VHE37_06605"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVT35233.1"
                     /translation="MKIWKLALVAGAALAFYLLLWPTGVEPVAWDAPLPPGPDTYPAN
                     DVLRHIQRIGAGVGLGPETVNVDAAGRVYTGYSDGRIVQLAPDGRTYMDLANTHGRPL
                     GITFGPDGGLVIADAGRGLLALGRKISVLADRADDVPFGLTNDVDNTVMDKNVYFTDS
                     TSKFAGTRYLDDLLEHGAHGRLMQYNVATGDTHVLLKDLHYANGVAVGPDDAYVLVSE
                     TSEYRVLRYWLKGDKAGTHDVFIDNLPGFPDNISYNNRDRFWLALAAPRNPQFDQLLS
                     GHLFERRLLSRLPSALRPQPQRRAFVLGLDLNGKVIANLQYAGADAYAPVTSVREFGP
                     WLYFGSLAERTIGRMPLHYAVASAPLPPSGWEKVPDSMQVPLPHSAESDREEEEEEEA
                     EQASKRAQKRPPH"
     gene            complement(1622..3868)
                     /locus_tag="VHE37_06610"
     CDS             complement(1622..3868)
                     /locus_tag="VHE37_06610"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVT35234.1"
                     /translation="MKRPALAVCASLPWLLAAAAHAATPANGTLSPAQPQLTYSSGPF
                     VAGNATAQTSPGNPTCQNPALPCDDFALTLSLPADYAATHPRALVTVSTYWAVTNQIA
                     YNLYLLDATGKVLNYVATGNDPVTLSLPAGSSTQNYTLRIVPQQAAGETPVTTVTLQG
                     DPTGGAASGLAPRFIQQQTPPGLGDNTNGEMNIGYNPLTGHVMTLGYTQTLRTTWPEV
                     RTPALPEACDALWEDVSDPDTQINTNDPILSTDPHTGRTFVSQLQVAAGESIFAYSDD
                     DGANWTLSPSTLDGGVDHQTVGGGPYPAGGSAAPSGSYPDMLLYCSQSIAAAFCIRSD
                     DGGATFGLPNILKTAADCGGFLGGIHGHVRIGDDGTAYVPDRNCNGVNTLHVSTDAGA
                     TWQHRPLPASATAGDGDPSVGVAHDGTVYFCYLNGDTHVHVAVSHDQGQSWINDRDIG
                     YAQGVIHAVFPQAVAGDGDRAACAFLGTTTPGNYQATDFTGIWHTYIATTYDGGNTWH
                     TVNPTPDDPVQGTGGICLGGTTCVQNRNLLDFNEITADDHGRVLFGFDDGCVSESCIT
                     TGVPDQVSIPGLSINSTAKTTVLRQSGGRTLLSQFDPAEPAAPKAACLAGARDASKAR
                     LTWKFPDNGGAAISAFRIFRGDAPGAESYLATTGAGAIYEDANAGSSVPKLYYRITAL
                     NSQGESVQSNEVELPVGDTLPDLPGGTTSGGTGAVPPAQDSGRYGGALDAMLLLPLLI
                     AARQRRVH"
     gene            complement(3873..>4243)
                     /locus_tag="VHE37_06615"
     CDS             complement(3873..>4243)
                     /locus_tag="VHE37_06615"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HVT35235.1"
                     /translation="AALVADISSGNAPLTVHLNASGSSDPSGDTITSYTFSFGDGSDP
                     VTQAGPTIAHTYGQDGLYAATVTAKNSHGVQSSNVARQLITVGAGAASGPSKGRFGGA
                     LPLVSVFMLMAAALVRRRRH"
BASE COUNT          591 a         1377 c         1561 g          714 t
ORIGIN      
        1 cctcgataat tcttgccctg attgtagtta tgacttgtcg ccgcatcccc aattcgttcg
       61 cggcgggtgg cgcatcctag caatccgccg cgctgcagcc agtcgggaaa aaccactagc
      121 gcgcgctgct cggcgtggag aaaacagatt acgcgcgcgc gctgcgcgta cactgcgcgc
      181 cgccatgaac ctgatcgtca acggtgaagc gcgcctgttg ccccccggca ccagcgtcca
      241 gatgctgatg gccgaactgg gcctggccgg caagcgcgcg gcggtggaac gcaacggcga
      301 gatcgtgccg cgctcgctct acgcgcagac cacgctggcc gaaggcgacc gcatcgagat
      361 cgtgcaggcc atcggcggcg gctagcggcg cggtcagtgc ggcgggcgct tctgcgcgcg
      421 tttgctcgcc tgttccgctt cttcttcctc ttcttcctcg cggtcggatt cggcgctgtg
      481 cggcagcggc acctgcatcg agtccggcac cttctcccag cccgacggcg gcagcggcgc
      541 gctggccacc gcgtagtgca gcggcatgcg gccgatggtg cgctcggcca ggctgccgaa
      601 atacagccat ggaccgaact cgcgcacgct ggtgactggc gcgtaggcgt cggcgccggc
      661 gtactgcagg ttggcgatga ccttgccgtt caggtcaagc ccgagcacga aagcgcgccg
      721 ctgcggctgc ggccgcagcg ctgagggcag gcgcgacagc agcctgcgct cgaacaaatg
      781 cccggacagc aattgatcga actgcgggtt gcgcggcgca gccagcgcca gccagaagcg
      841 gtcgcggttg ttgtagctga tgttgtcggg aaagccgggc aggttgtcga tgaacacgtc
      901 gtgcgtgccg gccttgtcgc ccttgagcca gtagcgcagc acgcggtact cgctggtctc
      961 ggacaccagc acgtaggcat cgtcggggcc gaccgccacg ccgttggcgt agtgcaggtc
     1021 cttgagcagc acgtgggtgt cgccggtggc gacgttgtat tgcatcaggc gcccgtgcgc
     1081 gccgtgctcc agcagatcgt cgaggtagcg cgtgccggcg aacttcgagg tcgagtcggt
     1141 gaagtagacg ttcttgtcca tcaccgtgtt gtcgacgtcg ttggtcaggc cgaacggcac
     1201 gtcgtcggcg cggtcagcca gcaccgagat cttccgtccc agtgcgagca gcccgcggcc
     1261 ggcgtcggcg atcaccaagc cgccgtccgg cccgaaggtg atgcccagcg gccgcccatg
     1321 ggtgttggcc agatccatgt aggtgcggcc gtcgggcgcg agctgcacga tgcggccgtc
     1381 gctgtagccg gtgtacacgc ggccggcggc gtcgacgttc accgtctccg ggcccagtcc
     1441 cactccggca ccgatgcgct ggatgtgcct gagcacgtcg ttggccgggt aggtgtccgg
     1501 gcccggcggc agtggcgcgt cccaggccac cggctcgaca ccggttggcc acagcagcag
     1561 gtaaaaggcc agcgccgcgc cggccaccag cgcgagtttc cagatcttca tggtgtgcag
     1621 cctagtgaac gcgccgctgc cgcgcggcga tcagcagcgg cagcagcagc atcgcgtcca
     1681 gcgcgccgcc gtagcgcccg ctgtcctgcg cgggcggcac agcgcccgtg ccgccgctag
     1741 tggttccgcc gggcaggtcg ggcagcgtgt cgcccacggg aagttcaacc tcgttgctct
     1801 gcacgctttc accctgcgag ttgagggcgg tgatgcggta gtagagcttg ggcacgctgc
     1861 tgccggcatt cgcatcttcg tagatggcgc cggcgccggt ggtggcaagg tagctctccg
     1921 caccgggcgc atcgccgcgg aagatgcgga acgcgctgat ggccgcgccg ccgttgtcgg
     1981 ggaatttcca ggtgaggcgc gccttgctcg cgtcgcgtgc gccggcgaga caggcggctt
     2041 tcggcgctgc gggctctgct ggatcgaact gcgacagcag cgtgcgccct ccggactggc
     2101 gcagcaccgt ggtcttggcg gtggagttga tcgacaggcc ggggatcgac acctggtccg
     2161 gcacgccggt ggtgatgcag ctttcgctga cgcagccatc gtcgaagccg aacagcacgc
     2221 ggccatggtc gtcggcagtg atctcgttga agtcgagcag gttgcggttc tgcacgcagg
     2281 tggtgccgcc caggcagatg ccgccggtgc cctgcaccgg gtcgtcgggc gtgggattca
     2341 ccgtgtgcca ggtgttgccg ccgtcgtagg tggtggcgat gtaggtgtgc cagatgccgg
     2401 tgaaatcggt cgcctggtaa ttgcccggcg tggtggtgcc gaggaaggcg caggccgcgc
     2461 ggtcgccgtc gccggccacg gcctgcggga acacggcatg gatcacgccc tgcgcatagc
     2521 cgatgtcgcg gtcgttgatc cagctctggc cctggtcgtg gctgaccgcg acgtgcacgt
     2581 gggtgtcgcc gttgagatag cagaagtaca ccgtgccgtc gtgcgcgacg cccaccgaag
     2641 gatcgccgtc gccggccgtg gcgctcgccg gcagcgggcg gtgctgccag gtggcgccgg
     2701 catcggtgga gacgtgcaga gtgttcacgc cattgcagtt gcggtcgggc acgtaggcgg
     2761 tgccgtcgtc gccgatgcgc acatggccgt ggatgccgcc gagaaaaccg ccgcagtcgg
     2821 cagcggtctt caggatgttg ggcaggccga aggtggcgcc gccgtcgtcg ctgcggatgc
     2881 agaacgccgc ggcgatcgac tgcgagcagt acagcagcat gtcgggatag ctgccgctcg
     2941 gcgccgcgct gccgccggcc gggtacggcc cgccgccgac ggtctggtgg tcgacgccgc
     3001 cgtcgagcgt gctcggcgac agcgtccagt tggcgccgtc atcgtcgctg taggcgaaga
     3061 tcgattcgcc ggcggcaacc tgcagctgcg agacgaaggt gcggccggtg tgcgggtcgg
     3121 tcgacaggat cgggtcgttg gtgttgatct gcgtgtcggg atcggacacg tcttcccaca
     3181 gcgcgtcgca ggcttcgggc agcgccggcg tgcgcacctc cggccaggtg gtgcgcaggg
     3241 tctgcgtgta gcccagcgtc atcacgtggc cggtgagcgg gttgtagccg atgttcatct
     3301 cgccgttggt gttgtcgccc agccccggcg gcgtctgctg ctggatgaag cgcggcgcca
     3361 gcccgctggc cgcgccgccg gtggggtcac cctgcagcgt gaccgtggtg accggcgttt
     3421 cgcccgcggc ctgctgcggc acgatgcgca gcgtgtagtt ctgcgtgctg ctgccagccg
     3481 gcagcgacag cgttaccgga tcattgccgg tggcgacgta gttgagcacc ttgccggtcg
     3541 cgtcgagcag gtacaggttg taggcgatct ggttggtcac cgcccagtag gtgctcaccg
     3601 tgaccagcgc ccgcggatgc gtcgccgcgt aatccgccgg cagcgacagc gtgagcgcga
     3661 agtcatcgca gggcagcgcc gggttctggc aggtgggatt gcccgggctg gtctgcgcgg
     3721 tggcgttgcc cgcgacgaac gggccggagc tgtacgtcag ctgcggctgt gcgggcgaca
     3781 gcgtgccatt ggccggcgtc gccgcatgcg cagctgccgc cagcagccac ggcagtgatg
     3841 cacagaccgc gagcgccggg cgcttcatgc gatcagtggc gccggcggcg aaccagcgcc
     3901 gccgccatca gcatgaacac cgagaccagc ggcagcgcgc cgccgaagcg gcccttgctc
     3961 gggccgctgg cagcgcccgc gcccacggtg atgagctggc gcgccacgtt gctgctctgc
     4021 accccgtggc tgttcttggc cgtgacggtt gccgcgtaga ggccatcctg gccgtaggtg
     4081 tgcgcaatgg tggggccggc ctgcgtcacc gggtccgacc catcgccgaa gctgaaggtg
     4141 tagctggtga tggtgtcgcc ggacggatcg ctgctgcccg aggcatttaa gtgcacggtc
     4201 agcggtgcgt tgcctgagga gatgtccgcc accagcgcgg cga
//
LOCUS       DASZZC010000317         4405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_602576,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000317 DASZZC010000000
VERSION     DASZZC010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602576"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /gene="ssb"
                     /locus_tag="VHE37_06620"
     CDS             complement(<1..127)
                     /gene="ssb"
                     /locus_tag="VHE37_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVT35236.1"
                     /translation="MARGVNKVILIGNLGKDPETRYFPSGDAVTNATLATTESWKD"
     gene            273..3095
                     /gene="uvrA"
                     /locus_tag="VHE37_06625"
     CDS             273..3095
                     /gene="uvrA"
                     /locus_tag="VHE37_06625"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HVT35237.1"
                     /translation="MDTIRIRGARTHNLKNVSLDLPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMQKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIYDYLRLLYARVGTARCPDHDVALEAQSVSQMVDQVLALPADSAWMLLAPVVRDRKG
                     EHAEWFEQMRAQGFVRARVDGRVYELDEIPTLNRKLKHNIDIVVDRFKVRPDAKQRLA
                     ESFETALRLTEGLAMVAPHGEAKGNELTFSSRMACPHCGYSLEELEPRLFSFNAPHGA
                     CPSCDGLGTLQVFDEARVVAHPELSLASGAIRSWDKRNPYTWHFITSLAKHYEFDPET
                     PFAELPSRIRGVVLRGSGEERLVFSYPDERGRLTSRTHRFEGIIPNLERRYRETESEA
                     VREELARYLSDKPCPDCKGQRLNRAARHVFVDGKNLPHITALSVRHAAEFFDELKLPG
                     HKGQIAEKILKEISARLGFLNNVGLDYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLETLFRLRDLGNTVIVVEHDEEAIRRADHVVDIGPGAGV
                     HGGEIVAQGKPREIEKNPRSITGHYLVGSEAIAVPKLRTPAQPKKQIVIRGARGNNLK
                     NVTASIPCGLMTCVTGVSGSGKSTLINDTLYGYAARQLNAASTQHAECDGIDGIDHVD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIRELFAGVPEARARGYEAGRFSFNVKGGRCE
                     TCEGDGVIKVEMHFLPDVYVTCDVCKGRRYNRETLEIKYKGKAINEVLDMTVEDGLEF
                     FKAVPAIARKLETLMDVGLSYITLGQNATTLSGGEAQRIKLAKELSRRDTGQTLYILD
                     EPTTGLHFHDVAQLLKVLERLREHGNTVVIIEHNLDVIKRADWVIDLGPEGGDGGGEI
                     IATGTPEDIAKNAKSHTGRFLKPLLK"
     gene            complement(3178..>4405)
                     /locus_tag="VHE37_06630"
     CDS             complement(3178..>4405)
                     /locus_tag="VHE37_06630"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT35238.1"
                     /translation="GATDSAVPFDSLKLQLEYPTNIQMYGFSFNTTAGDLSLQGEVAY
                     RPRDPLQVAVVDLAFAGFGPTLGYCHLKPGTALPSYSGGGTANGCLGTTGGMGISNSA
                     TPDNPAMTAYGNSDFINPDGSAGAYKDTFDLVVGAGVGSGRSFPSFIIPYRGMQSGTN
                     PGSDPSKPYDHNNPGYIRGWEYFRTFQFNLGGTYIMGATDNPIGADQIITLFETGATW
                     VPDMPSLDRLQFEAPGIYTHASAGADGSGFEFVNHPGVRPGIQPDGSYQQCGGTLGAC
                     RALGGQRQACSSIDDCSYGSDGLRFNPHQADPGLFPTKISAGYDMIMLVRYESVLPGI
                     SFENRIIWKHDVYGTAPGLATNFIEGRKNADLQIEMRYKSALAFDVGYTWFWGGGSQN
                     LNRDRDQVRTFVKYQF"
BASE COUNT          793 a         1419 c         1459 g          734 t
ORIGIN      
        1 tgtccttcca gctctcggtg gtggccagcg tggcgttggt gacggcgtcg ccgctgggga
       61 aataacgcgt ctcggggtct ttgccgagat tgccgatgag gatgaccttg ttgactccgc
      121 gtgccatgga gaatttctcc cttgtcttgc tgcttctgcg gacccagatt gagtcccatt
      181 cggacgggcc ttccccgtcc gtcctgcgtg cgctttgctt tgccgcgcgc gcgttgccta
      241 tagtactccg tttccccgtt tcgacctctg ccatggacac gatccgcatc cgcggcgcgc
      301 gcacgcacaa cctcaagaac gtctcgctgg acctgccgcg ggacaagctc atcgtcatca
      361 ccgggctgtc cggctcgggc aagtcgtcgc tggccttcga cacgctgtac gcggaaggac
      421 aacggcgcta cgtggaatcg ctgtcggcct atgcgcggca gttcctgtcg atgatgcaga
      481 agcccgacgt ggattccatc gagggcctgt cgccggccat ttccatcgag cagaaatcca
      541 cctcgcacaa cccgcgctcc accgtgggca cggtgaccga gatctacgac tatctgcgcc
      601 tgctctatgc gcgcgtgggc acggcgcgct gcccggacca cgacgtggcg ctggaagcgc
      661 agtcggtgtc gcagatggtg gaccaggtgc tggcgctgcc ggcggacagt gcgtggatgc
      721 tgctggcgcc ggtggtgcgc gaccgcaagg gcgagcacgc cgagtggttc gagcagatgc
      781 gcgcgcaggg attcgtgcgc gcgcgcgtcg acggccgcgt gtacgaactc gacgagatac
      841 ccacgctcaa ccgcaagctc aagcacaaca tcgacatcgt ggtggaccgc ttcaaggtgc
      901 ggcccgacgc caagcagcgc ctggcggaat cgttcgagac cgcgctgcgc ctcaccgaag
      961 gcctggccat ggtggcaccg catggcgagg ccaagggcaa cgagctgacg ttctcctcgc
     1021 gcatggcctg cccgcactgc ggctattcgc tggaagagct ggagccgcgc ctgttctcgt
     1081 tcaacgcgcc gcacggtgcc tgcccgtcat gcgacggcct gggcacgctg caggtgttcg
     1141 acgaggcgcg cgtggtggcg caccccgagc tgtcgctggc ctcaggcgcc attcgcagct
     1201 gggacaagcg caacccgtac acctggcatt tcatcacctc gctggccaag cactacgagt
     1261 tcgatccgga aacaccgttt gccgaactgc cctcgcgcat ccgtggcgtc gtgctgcgcg
     1321 gcagcggcga agagcgcctg gtgttttcgt accccgacga gcgcggccgg ctgaccagcc
     1381 gcacgcaccg cttcgagggc atcattccca atctcgagcg ccgctaccgc gaaaccgaat
     1441 ccgaagcggt gcgcgaagag ctggcgcgtt atctgtcgga caaaccctgc cccgactgca
     1501 agggccagcg cctgaaccgc gcggcgcggc atgtgttcgt cgacggcaag aacctgccgc
     1561 acatcaccgc gctttcggtg cgccacgcag cggaattttt cgacgagctg aaactgccgg
     1621 gccacaaggg ccagatcgcg gaaaagattc tcaaggaaat atccgcgcgc ctgggctttc
     1681 tcaacaacgt cggtctcgac tatctcacgc tgtcgcggtc ggcggaaacg ctgtcgggcg
     1741 gcgaggcgca gcgcatccgc ctggcctcgc agatcggcgc cggtctggtc ggcgtgatgt
     1801 acgtgctgga tgagccgtcg atcgggctgc accagcgcga caacgagcgc ctgctggaaa
     1861 cattgttccg cctgcgcgac ctgggcaaca cggtgatcgt ggtcgagcac gacgaggaag
     1921 ccatccgccg cgccgatcac gtggtcgaca tcggccccgg cgccggcgtg cacggcggcg
     1981 agatcgtggc gcagggcaag ccgcgcgaga tcgaaaagaa cccgcgctcg atcaccggcc
     2041 actacctggt cggcagcgaa gccatcgcgg tgccgaagct gcgcacgccg gctcagccga
     2101 agaagcagat cgtcatccgc ggcgcgcgcg ggaataacct gaagaacgtc accgcgtcga
     2161 tcccgtgcgg gctgatgacc tgcgtcaccg gcgtgtcggg ctcgggcaag tccacgctga
     2221 tcaacgacac gctctacggc tacgccgcac gccagctcaa tgccgcttca acccagcacg
     2281 ccgagtgtga cggcatcgat ggcatcgatc atgtcgacaa ggtgatcgat atcgaccagt
     2341 cgcccatcgg ccgcacgccg cgcagcaacc cggcgaccta caccgggctg ttcacgccga
     2401 tccgcgaact gttcgccggc gtacccgagg cgcgcgcgcg cggctacgag gccgggcgct
     2461 tctcgttcaa cgtcaagggc ggccgctgcg agacctgcga aggcgacggc gtgatcaagg
     2521 tggagatgca cttcctgccg gacgtgtacg tcacctgcga cgtgtgcaag ggccgccgct
     2581 acaaccgcga aacgctggag atcaagtaca agggcaaggc gatcaacgaa gtgctcgata
     2641 tgacggttga ggatggcctg gaattcttca aggcggtgcc ggccatcgca cgcaagctgg
     2701 aaaccttgat ggacgtgggc ctgtcctaca tcacgctggg ccagaacgcg accacgctgt
     2761 ccggcggcga ggcgcagcgc atcaagctgg ccaaggaatt gtcacgaaga gataccgggc
     2821 agacgctgta tatcctcgac gagccgacca ccggcctgca tttccacgac gtggcgcagc
     2881 tgctcaaggt gctcgaacgc ctgcgcgaac atggcaacac cgtggtgatc atcgagcaca
     2941 atctcgatgt catcaaacgc gcagactggg tgatcgacct gggccccgag ggtggcgacg
     3001 gcggcggcga gatcatcgcc accggtacgc cggaagacat cgccaaaaac gcgaagtcac
     3061 ataccgggcg cttcctcaag ccgctgctga agtaggggac gtcattcccg cgaaagcggg
     3121 aatgactggc aaaaaaatgg gccgcatccg cggcccattt ttgtcttacc ccgaatctca
     3181 gaactgatac ttcacaaacg tgcgcacctg atcgcggtcg cggttcaggt tctgcgaacc
     3241 gccgccccag aaccaggtgt agccgacgtc gaacgccagc gccgacttgt agcgcatctc
     3301 gatctgcagg tcggcgttct tgcggccttc gatgaagttg gtcgccaggc ccggtgccgt
     3361 gccgtagacg tcatgcttcc agatgatgcg gttctcgaag ctgatgcccg gcagcaccga
     3421 ctcgtagcgg accagcatga tcatgtcgta gccggccgag atcttggtcg ggaacaggcc
     3481 aggatcggcc tggtgcgggt tgaagcgcag gccgtcggag ccgtagctgc agtcgtcgat
     3541 cgacgagcaa gcctggcgct ggccgcccag cgcgcggcag gcgcccagcg taccgccgca
     3601 ttgctggtag ctgccgtccg gctggatgcc cgggcgcacg ccggggtgat tgacgaactc
     3661 gaagcccgag ccgtcggcgc cggcgctggc atgcgtgtag atgccgggcg cctcgaactg
     3721 caggcgatcg agcgagggca tgtccggcac ccaggtggcg ccggtttcga acagcgtgat
     3781 gatctggtcg gcgccgatcg ggttgtcggt ggcgcccatg atgtaggtgc caccaaggtt
     3841 gaactggaac gtgcggaaat attcccagcc gcggatgtag cccgggttgt tgtgatcgta
     3901 gggcttggac ggatcgctgc cgggattggt gcccgactgc atgccgcggt acgggatgat
     3961 gaacgacggg aacgagcggc cggacccgac gccggcgccc acgaccaggt cgaaggtgtc
     4021 cttgtacgcg ccggccgagc catcgggatt gatgaagtcg ctgttgccgt aagccgtcat
     4081 cgccgggttg tcaggcgtcg ccgagttgct gatgcccatg ccgcccgtgg tacccaggca
     4141 gccattggcg gtgccgccac ccgagtagga cggcagcgcc gtgcccggct tcagatggca
     4201 gtagccgagc gtcgggccga agccggcgaa cgccaggtca actacggcaa cctgcagcgg
     4261 atcgcgcggg cggtaagcga cttcgccctg cagcgagaga tcgccggccg tggtgttgaa
     4321 cgagaagccg tacatctgga tgttggtcgg atactcgagc tgcagcttga gcgagtcgaa
     4381 cggcactgcg ctgtcggtgg cgcca
//
LOCUS       DASZZC010000318         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_540714,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000318 DASZZC010000000
VERSION     DASZZC010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540714"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHE37_06635"
     CDS             complement(<1..295)
                     /locus_tag="VHE37_06635"
                     /inference="COORDINATES: protein motif:HMM:NF026065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HVT35239.1"
                     /translation="MSAFWSWFVVAGVVLVAGGSFVLLLLNARRKPGEAEDTGHVWDE
                     DLKENNHPLPRWWFNLFVITVLFAAGYLFLFPGMGSRNGSLGWTQAGQLKEQLD"
     gene            complement(292..459)
                     /locus_tag="VHE37_06640"
     CDS             complement(292..459)
                     /locus_tag="VHE37_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HVT35240.1"
                     /translation="MINGIVIVLVMSALGALTWWAWSPRRKSSFEQHARVALHDEPGA
                     ELPPCCKGERT"
     gene            complement(456..1067)
                     /gene="ccoO"
                     /locus_tag="VHE37_06645"
     CDS             complement(456..1067)
                     /gene="ccoO"
                     /locus_tag="VHE37_06645"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HVT35241.1"
                     /translation="MNHSTLEKNIGLLAAFTAVAISFGGLMEILPLMKQEAAARPGNV
                     TYLRTPLEVAGRDVYIREGCYNCHSQMIRSLPEEVLRYGAASQMQDSAYDHPFQWGSK
                     RTGPDLARVGGRYSDDWHRIHLMNPRDVVPESNMPSYHWLAEQKVDGDEVAAMMRALS
                     RVGVPYSEQQIAGAATEVDGKTKLDALIAFLQSLKPPVPESKS"
     gene            complement(1064..2494)
                     /gene="ccoN"
                     /locus_tag="VHE37_06650"
     CDS             complement(1064..2494)
                     /gene="ccoN"
                     /locus_tag="VHE37_06650"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HVT35242.1"
                     /translation="MDSTAPARPHYHDQVVRQFALMTVVWGIVGMAVGLFIAAELIWP
                     QLNVTQWLAYGRLRPLHTNAVIFAFGGCALFATSYWVVQRTCQTPLFLPRLAAFTFWG
                     WQTVIVLAAITLPLGMTQGKEYAELEWPIDILIAIVWVAYAVVFFGTIAQRKVSHIYV
                     ANWFYGSFIIAVAVLHIVNSAAIPISWTKSYSAYAGATDAMVEWWYGHNAVGFFLTAG
                     FLGMMYYFVPKQAGRPIYSYRLSIVHFWSLIAVYMWAGPHHLHYTALPDWVQSLGMVF
                     SLILLAPSWGGMINGIMTLSGAWHKLREDPILKFFIVSLSFYGMSTFEGPMMSIKTVN
                     ALSHYTDWTIGHVHSGALGWVAMISVGSIYMLIPRLYGKREMYSVPAINLHFWLSTIG
                     TVVYIAAMWIAGVMQGLMWRALNDDGTLTYSFIESLKATYPFYGMRLIGGAMFLSGML
                     VMAWNVWRTVRDEKPAVIAVPQGAHA"
     gene            2652..3143
                     /locus_tag="VHE37_06655"
     CDS             2652..3143
                     /locus_tag="VHE37_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HVT35243.1"
                     /translation="MSNAAAAVVEATPDFSVALFSVNHWMVIGATVMLVGMAVGLHYE
                     ALERLNARLPLWGLRARPRVLMLIISLLFIHTAEIWIFGGGIYLLVQHPGLGSVSGVP
                     GVQLLDTVYLSAVTFTTVGYGDLAPHGPLRFLLGSEALTGTVLVTWSASFTFLEMQRF
                     WRH"
     gene            3218..3874
                     /locus_tag="VHE37_06660"
     CDS             3218..3874
                     /locus_tag="VHE37_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVT35244.1"
                     /translation="MNADLKLQQWVIDELEFDPRVDAAHIGVTARDGVVTLSGHVAHY
                     AQKIAAEEAARRVHGVRAVAQQIRVEFAFDKKIADDEIAARVVKLLEWSVHPEKGRIE
                     VTVERGVVTLSGVVEWEFQRRDAEAQVRRLGGVTDVVNDIRLDPHLEAGNIRQQINRA
                     LERAGQVDAARVEVDVVDARRIALRGSVHNLADKALVENAAWQVPGVVRVDNHLAVGS
                     "
BASE COUNT          675 a         1223 c         1351 g          654 t
ORIGIN      
        1 cgtcgagctg ttctttcagc tggccggcct gggtccagcc gagcgagccg ttgcggctgc
       61 ccatgccggg aaacaggaac aggtagccgg cggcgaacag cacggtgatg acgaacaggt
      121 tgaaccacca gcgcggcagc gggtggttgt tctccttgag atcctcgtcc cacacgtggc
      181 cggtgtcctc ggcctcaccc ggtttgcgcc gtgcgttgag cagcagcagc acgaaggaac
      241 cgccggccac cagcacgacg ccggcgacga cgaaccagct ccagaacgcg ctcatgtgcg
      301 ctctcccttg cagcagggcg gcagttcggc gccgggctcg tcgtgcagcg ccacgcgcgc
      361 gtgctgctcg aagctgctct tgcggcgcgg cgaccaggcc caccaggtga gggcgccgag
      421 cgcgctcatc accaggacga tgacgatgcc gttgatcatg acttgctctc cgggacgggg
      481 ggcttgaggg attgcaggaa cgcgatcagc gcgtcgagct tggtcttgcc gtcgacctcg
      541 gtcgcggcgc cggcgatctg ctgctcgctg tagggcacgc ccacgcgcga cagcgcgcgc
      601 atcatcgcgg ccacttcgtc gccgtcgacc ttctgctccg ccagccagtg gtaggagggc
      661 atgttcgact ccggcaccac gtcgcgcgga ttcatcaggt ggatgcggtg ccagtcgtcg
      721 ctgtagcggc cgccgacgcg cgccaggtca ggcccggtgc gcttgctgcc ccactggaac
      781 gggtggtcgt aggccgagtc ctgcatctgc gaggcagcgc cgtagcgcag cacttcctcg
      841 ggcagcgaac gaatcatctg cgagtggcag ttgtagcagc cctcgcggat gtagacgtcg
      901 cgcccggcca cttccagcgg cgtgcgcagg taggtgacgt tgccgggcct ggccgcagct
      961 tcctgcttca tcagcggcag gatctccatc aggccgccga agctgatggc gacggcggtg
     1021 aaggcggcca gcaggccgat gttcttttcc agcgttgagt ggttcatgcg tgggctcctt
     1081 gcggcaccgc gatgaccgcg ggcttttcat cgcgcaccgt gcgccacacg ttccacgcca
     1141 tcaccagcat gccggacagg aacatggcgc cgccgatcag gcgcatgccg tagaacggat
     1201 aggtggcctt gagcgattcg atgaaggagt aggtgagcgt gccgtcgtcg ttgagcgcgc
     1261 gccacatcag gccctgcatc acgccggcga tccacatggc ggcgatgtag accaccgtgc
     1321 cgatggtgga cagccagaaa tgcaggttga tggccggcac gctgtacatc tcgcgcttgc
     1381 catacaggcg cgggatcagc atgtagatgg agccgaccga gatcatcgcc acccagccca
     1441 gcgcgccgga atgcacgtgg ccgatggtcc agtcggtgta atgcgacagc gcattcaccg
     1501 tcttgatcga catcatcggc ccttcaaacg tgctcatgcc gtagaacgac agcgagacga
     1561 tgaagaactt gaggatcggg tcttcgcgca gcttatgcca cgcacccgac agggtcatga
     1621 tgccgttgat catgccgccc cagctcggcg ccagcaggat cagcgagaac accatgccca
     1681 ggctctgcac ccagtcgggc agcgcggtgt agtgcaggtg atgcgggccg gcccacatgt
     1741 acaccgcgat cagcgaccag aaatgcacga tcgacaggcg gtacgagtag atcggccgcc
     1801 cggcctgctt gggcacgaag taatacatca tgccgaggaa gccggcggtg aggaaaaatc
     1861 ccaccgcgtt gtggccgtac caccactcca ccatggcatc ggtggcgccg gcgtaggccg
     1921 agtaggactt ggtccacgag atcgggatgg ctgcgctgtt gacaatgtgc agcaccgcaa
     1981 cggcgatgat gaacgagccg tagaaccagt tggccacgta gatgtgcgac accttgcgct
     2041 gcgcgatggt gccgaagaac accacggcgt aggccaccca gacgatggcg atgaggatgt
     2101 cgatcggcca ttccagctcg gcgtattcct tgccctgggt catgcccagc ggcagcgtga
     2161 tggcggcgag gacgatcacc gtctgccagc cccagaaggt gaacgcggcg agcctgggca
     2221 ggaacagcgg cgtctggcag gtgcgctgca ccacccagta cgaggtggcg aacagcgcgc
     2281 agccgccgaa ggcgaagatc accgcgttgg tgtgcagcgg gcgcaggcgg ccgtaggcca
     2341 gccactgggt gacgttgagt tgcggccaga tcagttcggc ggcgatgaac aggccgacgg
     2401 ccatgccgac gatgccccag accacggtca tcagcgcgaa ctggcggacg acctggtcgt
     2461 ggtaatgcgg ccgcgcggga gcggtggaat ccatcgtgcc tccgggtttt ggacggcggc
     2521 attgttttca tgcgggctgc ccgcagcatt gacctgggtc aatcgtcagc ccgttttttc
     2581 ctgctgacct gcgtcaatgc cggcacgcgg cgcgctgcgc aggatgcgcg gcaacatcca
     2641 gggagctgtt gatgagcaat gccgctgcag ccgtggtcga ggcgacgccg gatttttccg
     2701 tggcgctgtt ttcggtcaac cactggatgg tgatcggcgc caccgtgatg ctggtgggca
     2761 tggccgtcgg cctgcattac gaggcgctgg aacggctcaa cgcgcgcctg ccgctgtggg
     2821 gcctgcgtgc ccgtccgcgc gtgctgatgc tgatcatcag cctgttgttc attcacaccg
     2881 cggaaatctg gatcttcggc ggcggcatct acctgctggt gcagcacccg ggtctgggct
     2941 cggtgtccgg cgtgccgggc gtgcaactgc tcgacacggt gtacctgtcg gcagtgacct
     3001 tcaccaccgt gggttacggc gacctggcgc cgcacgggcc gctgcgcttc ctgctcggca
     3061 gcgaggcgct gaccggcacc gtgctggtca cctggagcgc gtcgttcacc ttcctcgaga
     3121 tgcagcgctt ctggcggcac tgagcctcgc ttgatccacg tcaatgccgc gcgcaccgct
     3181 cgcggcaacc atgcagcagc caatgatgga gttgacgatg aacgccgacc tgaagctgca
     3241 gcagtgggtg atcgacgaac tggaattcga cccgcgcgtg gacgccgccc atatcggcgt
     3301 gacggcgcgc gacggcgtgg tgacgctcag cggccacgtg gcgcattacg cgcagaagat
     3361 cgcggccgag gaggcggcgc ggcgcgtgca cggcgtacgc gcggtggcgc agcagatccg
     3421 cgtggagttc gctttcgaca agaagatcgc cgacgacgag atcgcggcgc gggtggtcaa
     3481 gctgctcgaa tggagcgtgc acccggagaa gggccgcatc gaggtcaccg tcgagcgtgg
     3541 agtggtcacc ttgtccggcg tggtcgaatg ggaattccag cggcgcgatg ccgaggcgca
     3601 ggtgcggcgc ctgggcggcg tgaccgacgt ggtcaacgac atccggctcg atccgcacct
     3661 ggaagccggc aacatccgcc agcagatcaa ccgcgcactt gaacgcgccg ggcaggtgga
     3721 tgccgcgaga gtcgaagtgg atgtggtcga cgcgcggcgc atcgcgctgc gtggcagcgt
     3781 ccacaacctg gccgacaagg cgctggtgga aaacgcggcg tggcaggtgc cgggcgtggt
     3841 gcgggtggac aatcacctgg cggtaggaag ctgatttttg gccccaccgc atggcttgtt
     3901 ttc
//
LOCUS       DASZZC010000319         6342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_990979,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000319 DASZZC010000000
VERSION     DASZZC010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990979"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..986)
                     /locus_tag="VHE37_06665"
     CDS             complement(111..986)
                     /locus_tag="VHE37_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35245.1"
                     /translation="MNASESQATLTLQDGRKLAYAEVGDRQGKPVLLLTGTGTGRSQA
                     YWFDAAARKAGVRLIVTDRPGYGHSDPKAELSFLNHADDVRELLDYLKLSRVAVAGMS
                     GGGGYAMACGYRMPERIERVVMVCGMVPAPPEVYRKMSGAVRMIFWLTIHMPRLATSL
                     LNRMQGGGLDPDGAAFKRKLKQVPEADRRVLGLSGMPELCYGAPARDALRQGMGIFVR
                     EMALYGKPLGFELSQVRVPVRVWHGLQDANVVIDIARYVASSVPGAKLTIEPECAHLF
                     GFGDPDGMMQQMIAP"
     gene            1209..1781
                     /locus_tag="VHE37_06670"
     CDS             1209..1781
                     /locus_tag="VHE37_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HVT35246.1"
                     /translation="MGELTMQRLAERLDVRRAAVHRHFSSREVLIAAVGEQAVASFQP
                     QPIDAEHWRAWLKDAFLQLIGFMRRNPFVLALRSWDQTAWAILPFVEAAAETLVKAGF
                     SEEQALHLWRMVFHYAYSAACSIQASPSSRHRYRAAERFSASGKFPHLAAVLPKLNED
                     PALSFARSLEWLLSGLPEPRATRTRKGRRK"
     gene            complement(1811..2080)
                     /locus_tag="VHE37_06675"
     CDS             complement(1811..2080)
                     /locus_tag="VHE37_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HVT35247.1"
                     /translation="MATGWAREGAVQEQIDATVKDAIARARAPLAAGPSLSHCAECGE
                     TIPAARRQAIPGVRFCVACQQEQDKNRSGAFSGYNRRGSKDSQLR"
     gene            2252..3091
                     /locus_tag="VHE37_06680"
     CDS             2252..3091
                     /locus_tag="VHE37_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT35248.1"
                     /translation="MSLKGKTLFITGGSRGIGLAIAVRAARDGANVAIAAKTAEPHSK
                     LPGTIYTAVDEINAAGGKGLAIQCDIRDEAQVQAAVKQTAETFGGIDVLVNNASAISL
                     TGTLATDMKRYDLMNQINTRGTYLAGRTCIPYLKKSANPHILTLSPPLDMQAKWFAPH
                     VAYSIAKYGMSLCAMAWAEEFRKDGVASNALWPRTTIATAAVRMIGGDPILKQSRTPE
                     IMADAAHVILSKPSRDFTGQFCIDDIVLHQAGVRDFSKYAAVPGTTKFAPDFFLPDNL
                     PEV"
     gene            3102..3599
                     /gene="greB"
                     /locus_tag="VHE37_06685"
     CDS             3102..3599
                     /gene="greB"
                     /locus_tag="VHE37_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HVT35249.1"
                     /translation="MSRYRPPAEKSSPYITAEGHARLKAEFDQLWRVRRPEVVKALSA
                     AAAEGDRSENAEYIYRKKELREIDARLKYLTLRLEEVKVAGAKPADAKKVFFGAWVEV
                     ADESDARKTWRIVGADEAEVARGLISVDSPVARALLGRTLGEVITVQLPAGESELEIL
                     AVRYE"
     gene            complement(3821..4435)
                     /locus_tag="VHE37_06690"
     CDS             complement(3821..4435)
                     /locus_tag="VHE37_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35250.1"
                     /translation="MKKLITMALMAACAAPGLALASTVDLDAFGSYGDAKFDNGYSPS
                     GGGGGIRARVNVPFTTAFFTGEYQSNKMDDSSFTPSHVTLDDTRVGVGSEISAGPLSV
                     FGRAEYVHMAFKPDTFPTDNSDGYGLHVGVDAGVGIAGVYGSAGYVHTSDTKGPELLV
                     GGRVTLLPLVQLFAEYRYTELNDNNGSNNDGHLNDYRAGVRVSF"
     gene            complement(4529..4897)
                     /locus_tag="VHE37_06695"
     CDS             complement(4529..4897)
                     /locus_tag="VHE37_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVT35251.1"
                     /translation="MAAAHAPGFLALVNDAKTRIREVHASVMPNILDKDKAARLLDTR
                     EESEYAAGHVKGAEWMGKGVIERDIEARHPDKNEPLYLYCGGGFRSALAADNLQKMGY
                     KNVYSIDGGWRVLKDLLPVE"
     gene            4993..5781
                     /locus_tag="VHE37_06700"
     CDS             4993..5781
                     /locus_tag="VHE37_06700"
                     /inference="COORDINATES: protein motif:HMM:NF006123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT35252.1"
                     /translation="MDTRKAIFITGAGAGIGRATALLFAARGWFVGLYDVDEAAVQKL
                     AGELGANAVCGKLDVSDYAQFEAALVQFMRASGGRLDVMFNNAGIVAVANFEDLALAR
                     LHRLVDVNFKGVINGCHAALPHLKRTPGARVISMCSASAIYGAPSFAVYSATKFAVRG
                     LTEALDVEWSRFGIRVMDVMPLFVNTPMVTEHFDSTPKSLERLGLKLQADDIARTVWK
                     AATRPRWLTHVHWLPGVQTWLLYLANKFSPAFLNRYTTKAVSGY"
     gene            5784..>6342
                     /locus_tag="VHE37_06705"
     CDS             5784..>6342
                     /locus_tag="VHE37_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35253.1"
                     /translation="MGYPVVLIHGMWCTPKNWDRIIGLLTPRGYDCHAPSLPAHLPGP
                     GQEQQVGAKSLREYVDFLEDYIRSKNFSQPPIIIGHSMGGFLAQAVAARIKVLALVLL
                     TPAAPAGIFPLRLKNTFAFLPHFLRWGFWRKAYKLSPASARKYAYNGLPAAQQERLYE
                     GLVYESGRVAFELGMWYLDFGGAARI"
BASE COUNT         1100 a         2120 c         2031 g         1091 t
ORIGIN      
        1 cgctgcggtt ggctccactt ctgcatccgt tgcccgagcg actaggggca gcgcagccac
       61 cgccagcgcc agcgtgccga gcgaaaagtt cgcgactgcc ttgcccaaag tcacggcgca
      121 atcatctgct gcatcatgcc gtcaggatcg ccaaagccga acaggtgcgc gcactcgggc
      181 tcgatcgtca gtttcgctcc aggaacactc gaagccacgt agcgcgcgat gtcgatcacc
      241 acgttcgcat cctgcagtcc gtgccatacg cgtaccggca cccggacctg ggaaagctcg
      301 aatcccagcg gcttgccgta aagggccatc tcacggacga agatgcccat gccctgccgc
      361 aaggcgtcgc gggccggtgc tccgtagcac agttccggca tgccgctcag ccccaacacg
      421 cggcgatccg cctccggtac ctgcttgagc ttgcgcttga aagccgcgcc gtcggggtcg
      481 agtccgccgc cctgcatgcg gttcagcagc gaggtcgcca ggcggggcat gtggatggtg
      541 agccagaaga tcatgcgcac cgctcccgac attttcctgt acacctccgg cggcgccggc
      601 accatgccgc agaccatcac cacccgctcg atgcgctcgg gcatgcggta gccgcaggcc
      661 atcgcatagc cgccgccgcc cgacatgccg gcaacggcta cgcgcgaaag tttcagatag
      721 tccagcaact cacgcacatc gtcggcatgg ttgaggaacg agagttccgc tttcgggtcg
      781 gagtgcccat aaccggggcg gtccgtaacg atcaggcgca cgcccgcctt gcgcgccgcg
      841 gcatcgaacc agtacgcctg ggatcgcccg gtgccggtac ccgtcagcag aagtaccggc
      901 ttgccttgcc tgtcgccgac ctccgcgtat gccagcttgc ggccgtcctg cagggtcaat
      961 gtcgcttgag attcgctggc attcatcatc aaatcccctg gcagaaaacg tcgctgcttc
     1021 gcgccccatg cacgcgaaac cgatcatttg ttgcacgtgc cattaaatat ataacataca
     1081 ctcgacacaa aatgaaaaga ccccacttgc ctgcttcctt ggcaacccat gtgcccggca
     1141 aacctcgccg cgggcgcccg ccgcgcatta cgcgcgcact catccttgcg gaagcgcaaa
     1201 agctgccgat gggagagttg accatgcagc gcctggccga gcggctggat gtgcgccgcg
     1261 ccgcggtgca tcgccacttc tcctcgcgcg aggtgctgat cgccgcggtc ggcgagcagg
     1321 ccgtggcgag ctttcagccg caacccatcg acgcggagca ctggcgcgcc tggctgaagg
     1381 atgcgttcct gcaattgatc gggttcatgc gcagaaaccc cttcgtcctg gcactgcgct
     1441 cctgggatca gacggcgtgg gccatcctgc cgttcgtgga agccgccgcg gaaaccctgg
     1501 tgaaggccgg gttcagcgaa gagcaggcgc tgcacctctg gcgcatggtt tttcactatg
     1561 cctacagcgc cgcctgctcg atccaggcga gtcccagcag caggcatcgc taccgcgcag
     1621 ccgagcgctt cagcgcttca ggaaaatttc cgcaccttgc cgcggttctg ccgaagttga
     1681 acgaggatcc ggcgctgagt tttgcccgct cgctggaatg gctgctttcc gggctgccgg
     1741 agccacgggc aacccggacc cggaaaggaa ggcggaagta gatccgccac gggccgaagc
     1801 cggtcaaccg tcaccgcagc tggctgtcct tgctcccgcg ccggttgtag ccgctgaacg
     1861 cgcccgaccg gttcttgtcc tgctcctgct ggcaggccac gcagaaccgc acgcccggga
     1921 tcgcctgccg ccgcgctgcc gggatcgttt caccgcattc ggcgcaatgg ctcaggctgg
     1981 ggccggcggc gagaggcgcg cgcgcccgcg cgatggcgtc cttgaccgtc gcgtcaatct
     2041 gctcctgtac cgcgccttcc cgtgcccagc ccgtcgccat ggcaaatcgt tcctcatgcg
     2101 cctgtccatc agagtggatg cacggcggcg gagtttcaag cctgttatgc ggcggcgcac
     2161 ggcggccatg ctgacaatgt ggattgtttc tgtttcgccc ggacaatccg caataatccg
     2221 ctccccaaat ttccggaacg cggagcgcac catgtccctc aaaggcaaga ccctgttcat
     2281 caccggcggc agccgcggca tcgggctggc catcgcggtg cgcgccgcgc gcgacggcgc
     2341 caacgtggcc atcgccgcca agaccgccga gccgcactcg aagctgcccg gcaccatcta
     2401 caccgcggtg gacgagatca acgccgccgg cggcaagggt ctggccatcc agtgcgacat
     2461 ccgcgacgaa gcgcaggtgc aggccgcggt gaagcagacg gcggagacct tcggcggcat
     2521 cgacgtgctg gtgaacaacg ccagcgccat ctcgctcacc ggcacgctgg ccaccgacat
     2581 gaagcgctat gacctgatga accagatcaa cacgcgcggc acctacctgg ccggccgcac
     2641 ctgcattccg tacctgaaga aatccgccaa cccgcacatc ctcacgctgt cgccgccgct
     2701 ggacatgcag gccaagtggt ttgcgccgca cgtggcgtac tccatcgcca agtacggcat
     2761 gagcctgtgc gccatggcct gggccgagga gttccgcaag gacggcgtcg ccagcaatgc
     2821 gctgtggccg cgcaccacca tcgccaccgc ggcggtgcgc atgatcggcg gcgacccgat
     2881 cctcaagcag tcgcgcacgc cggagatcat ggccgacgcg gcgcacgtga tcctgagcaa
     2941 gccttcgcgc gatttcaccg gccagttctg catcgacgac atcgtgctgc accaggccgg
     3001 cgtgcgtgat ttttccaaat atgcggcggt gccgggcacc accaagttcg cgcccgactt
     3061 tttcctgccg gacaacctgc ccgaagtctg agccggggcc tgtgtcgcgc taccggccgc
     3121 ccgcggaaaa atcctcgccg tacatcacgg cggaggggca cgcgcggctg aaggcggagt
     3181 tcgaccagct gtggcgcgtg cgccgcccgg aagtggtgaa ggcgctgtcg gcggcagccg
     3241 cggaaggcga ccgcagcgag aacgcggagt acatctaccg caagaaggaa ttgcgcgaga
     3301 tcgatgcgcg gctcaagtac ctgacgctgc gcctggagga agtgaaggtg gccggcgcaa
     3361 aaccggccga tgcgaagaag gttttcttcg gcgcctgggt cgaggtggcg gatgagagcg
     3421 atgcgcgcaa gacctggcgc atcgtcggcg ccgacgaggc cgaggtggcg cgcgggctga
     3481 tcagcgtgga ttcgccggtg gcgcgcgcgc tgctcgggcg cacgctgggc gaggtgatca
     3541 cggtgcagct gccggcgggc gagagcgaac tggaaattct cgccgtgcgc tacgagtgac
     3601 gcgcgcaggc gcaggaaaat gctccaccgc accggccgcc agcgtgttat aataaataat
     3661 atgattatta aaataatcaa taaaacataa catcaatacc gggcgtgcgg ttgccggttt
     3721 ttgctcgcgt ggctcggcgc acggcgccaa cgactgcccg ggatcaccgc aataaaaaag
     3781 ggccgcgcgg gcggcccttc gatgcagcag gggtgaagct tcagaagctc acgcgcacgc
     3841 cggcgcgata gtcgttgagg tgaccgtcgt tgttgctgcc gttgttgtcg ttgagctcgg
     3901 tgtagcggta ctcggcgaac agctgcacca gcggcagcaa ggtcacgcgg ccgccgacca
     3961 gcagttccgg tcccttggtg tcggaggtgt gcacgtagcc ggccgaaccg tagacgccgg
     4021 cgatgccaac accggcatcg acgcccacgt gcaggccgta gccgtcggag ttgtcggtgg
     4081 ggaaggtgtc gggcttgaat gccatgtgca cgtactcggc gcggccgaac accgacagcg
     4141 ggcccgcgga gatttcagaa cccacgccca cgcgcgtatc gtcgagcgtg acgtgcgacg
     4201 gcgtgaagct gctgtcatcc atcttgttgc tctggtactc gccggtgaag aacgccgtgg
     4261 tgaagggcac gttgacgcgt gcgcggatgc cgccgccgcc gccgctcgga gagtagccgt
     4321 tgtcgaactt cgcatcgccg taggaaccaa aggcgtccag atccacggtg gaggcgaggg
     4381 ccagtccggg cgccgcgcat gccgccatca gggccattgt gatcagcttt ttcatgccat
     4441 ctccttgcat acgcccctgg tgggatcgca cagcgtgccg gcaggggccg taccggcgct
     4501 gttttggacc gctactgctg gctaaaagtc attcgaccgg cagcaggtcc ttgagcacgc
     4561 gccagccgcc atcaattgag tagacgtttt tgtagcccat cttctgcagg ttgtccgccg
     4621 ccagcgccga gcggaagccg ccgccgcaat acaggtacag cggttcgttc ttgtcgggat
     4681 gccgcgcctc gatgtcgcgt tcgatcacgc ccttgcccat ccactcggcg cccttgacgt
     4741 ggccggcggc gtattcgctt tcctcgcgcg tgtcgagcag gcgcgcggcc ttgtccttgt
     4801 ccaggatgtt gggcatgacg ctggcgtgga cttcgcggat gcgcgtcttc gcatcgttga
     4861 ccagcgccag gaaaccgggt gcgtgtgcag cggccatctg catcctcctt gattcgatgg
     4921 gcccgcattt tagccgcaag gtcgcgtgct agattaccgc ccacaaaaaa ttccgcttcc
     4981 cgaggagagc gtgtggacac gcgcaaggcc atattcatca ccggcgccgg cgccggcatc
     5041 ggccgcgcca ccgcgctgct gttcgccgcg cgcggctggt tcgtcggact gtacgacgtg
     5101 gatgaagcgg cggtgcagaa gctggccggc gaactcggcg ccaatgccgt ctgcggcaag
     5161 ctcgacgtga gtgactacgc gcagtttgag gcggcgctgg tgcagttcat gcgcgcctcc
     5221 ggcgggcgac tcgacgtgat gttcaacaat gccggcatcg tcgccgtggc caatttcgaa
     5281 gacctggcgc tggcgcggct gcaccgcctg gtcgacgtca acttcaaggg cgtgatcaac
     5341 ggctgccacg ccgcgctgcc gcacctgaag cgcacgccgg gcgcgcgcgt gatcagcatg
     5401 tgctcggcct cggccatcta cggcgcgcca tcgttcgccg tgtattccgc caccaagttc
     5461 gcggtgcgcg gcctgaccga ggcgctcgac gtggaatgga gccgcttcgg catccgcgtg
     5521 atggacgtga tgccgctgtt cgtcaacacg ccgatggtga ccgagcattt cgacagcacg
     5581 ccgaaatctc tcgagcgcct gggcctcaag ctgcaggccg acgacatcgc gcgcaccgtg
     5641 tggaaggcgg ccacgcgccc gcgctggctc acccacgtgc actggctgcc gggcgtgcag
     5701 acctggctgc tgtacctggc caacaagttt tcaccggcat ttctcaaccg ctacaccacc
     5761 aaggctgtct cgggatactg aacatgggtt atccggtcgt cctcattcac ggcatgtggt
     5821 gcacgccgaa aaactgggat cgcatcatcg gcctgctcac gccgcgcggt tacgactgcc
     5881 acgcgccgag cctgccggcg catctgcccg gcccgggcca ggagcagcag gtgggcgcga
     5941 agagcctcag ggaatacgtg gattttctcg aggactacat ccgctcgaag aatttttcgc
     6001 agccgccgat catcatcggc cactccatgg gcggcttcct cgcgcaggcg gtggccgcgc
     6061 gcatcaaggt gctggcgctg gtgctgctca caccggccgc gccggccggc atcttcccgc
     6121 tccggctgaa gaacaccttc gccttcctgc cgcatttcct gcgctggggt ttctggcgca
     6181 aggcctacaa gctgtcgccg gcaagcgcgc gcaagtacgc ctacaacggc ctgccggcgg
     6241 cgcagcagga acgcctgtac gaaggactgg tctatgaatc cggccgcgtc gctttcgagt
     6301 tgggaatgtg gtacctggat ttcggcggcg ctgcgcgcat cg
//
LOCUS       DASZZC010000320         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_928405,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000320 DASZZC010000000
VERSION     DASZZC010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928405"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..287
                     /gene="yacG"
                     /locus_tag="VHE37_06710"
     CDS             66..287
                     /gene="yacG"
                     /locus_tag="VHE37_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HVT35254.1"
                     /translation="MIKNNIENGNAVGPLGRCPQCSQPAQLGAQNPWRPFCCERCRQL
                     DFGAWIAERYTIAESGEETPPAPADHQLQ"
     gene            complement(275..1201)
                     /locus_tag="VHE37_06715"
     CDS             complement(275..1201)
                     /locus_tag="VHE37_06715"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HVT35255.1"
                     /translation="MRPVIPVACGALVDAEGAVLIARRPSGKIAAGKWEFPGGKIEAG
                     ESAGAALARELHEELGITVQRARPLIRITHDYSDRTVVLDTWRVEQWSGELHCREGQQ
                     VAWVRPEQLHQHDLLAADGPIVRALQLPSIYVFTPPQANAGQVLAGVDRLPRGALLRL
                     RLPQLADDAYETLAQRVIERGRGLRVMLDRSVELVQRVGAAGLHLSAARVAAQESRRL
                     PEPFLLGGSAHNAGECTALRRAGADFAVLGPVLPTATHPQSATLGWSDFGLLATKANL
                     AVYAIGGVGPDDLAQAHACYAQGVAGISAYWS"
     gene            complement(1201..2418)
                     /gene="argJ"
                     /locus_tag="VHE37_06720"
     CDS             complement(1201..2418)
                     /gene="argJ"
                     /locus_tag="VHE37_06720"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HVT35256.1"
                     /translation="MAVNLAVPETLHPVAGVRLGVAEAAIKKAGRKDLLLIALDAGTQ
                     VAGVFTQNAFAAAPVQVCRKQLAPGGIRGLLINSGNANAATGEGGIEDALASCRAAAL
                     ALGCKPEQVLPFSTGVIGQRLPVQRMVDAAPKACAALRADGWPDAMRAIMTTDTVPKG
                     ATRKVETSQGVVTVTGIAKGVGMIHPDMATMLAFIGTDAHLTAQATRQALESAVSHSF
                     NCVTVDGDTSTNDSCMLFATGKVGTRAIDPAHADFAAIAGGIEAVCRELAHAIVRDGE
                     GATKFITIEVSGARSTAEARQVALSVAHSPLFKTAMFASDANWGRIVMAIGKAGVHGL
                     DPAGVSLALDDVQLLRGGQPHPGYREELGAAVVARPEFSIRIGLGRGEASATVWTCDF
                     SYDYVKINAEYRT"
     gene            complement(2437..5208)
                     /gene="secA"
                     /locus_tag="VHE37_06725"
     CDS             complement(2437..5208)
                     /gene="secA"
                     /locus_tag="VHE37_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HVT35257.1"
                     /translation="MFNNMLARVFGSRNQRILKRLDSFVTEVGRLEAKFRDLPDAELS
                     RQTQLFRERLAGGEALESLKAEAFAVVREAGRRVLGMRHFDVQLVGGAILHEGKIAEM
                     KTGEGKTLVSTLPAYLNALPAKGVHVVTVNDYLAKRDAEWMGRIHRFLGLTVGVVVPG
                     QTQAEKREAYAADITYATNNELGFDYLRDNMAFSLADKVQRGHYYAIVDEVDSILIDE
                     ARTPLIISGPTDDDPELYRKINAFVPRLQRQDNEEGPGDFYADEKAKQVHLSEAGHEK
                     VETFMRQAGLLGEEDSLYDAANIVLVHHLNALLRAHHLYRRDVEYIVRNGEIIIVDEF
                     TGRMMPGRRWSDGLHQAIEAKENVKIQQENQTLASITFQNYFRLYKKLSGMTGTADTE
                     AYEFQNIYNLEVVVIPPNRAMVRKDAGDQVFMTAREKFQAVVAEIRQAHQKQQPVLVG
                     TASIETSELLSGLLAKDGIKHDVLNAKQHEREAEIVANAGLPGTVTIATNMAGRGTDI
                     VLGGNLETELARLPLEDNVAREAARSAWQQRHDAVVAAGGLYVIGSERHESRRIDNQL
                     RGRSGRQGDPGASRFYLSLEDTLMRIFTPPSMRAMLQRLGMAEGEAIEHRWVTRAIET
                     AQRKVEGHNFDIRKNLLEYDNVANDQRKAIYELRDELMGAERVSDRVEALRPDVVNAV
                     IDAYIPPQSIEQEWNAKGLEEALRRDFALDLPVAQWLDQEKGLTEKALRERIVERVGA
                     AYSQKKEQAGAAVLEHFEKAVMLQVLDNLWREHLAAMDYLRQGIHLRGYAQKDPKQEY
                     KREAFGMFNDMLARYKHEVITLLARVRVQTEAEVAEIEAQRRRQAEANEGRLQFQHAA
                     APVAGDAPAPGESPAPPGADAARPLQQRAAPPARPATVVREQPKVGRNDPCPCGSGKK
                     YKQCHGALV"
     gene            complement(5307..>6110)
                     /locus_tag="VHE37_06730"
     CDS             complement(5307..>6110)
                     /locus_tag="VHE37_06730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVT35258.1"
                     /translation="GLGYLARGDGSVLPSGLVATWAEEVSAQHSELNKAREEAEENAR
                     ALARRIAQLHAHMMRVNAAGARLTEIAGLDRGEFNFDQAPAVGGPEEASAQETGLVQV
                     ISSLDQFEAQLSDRERQMRVLEDLLLASRLQKQVKPSGWPVDNGWISSTFGWRTDPFT
                     GRYAQHTGIDFAAREGADVLAVATGIVTEAGERFGYGNLVEINHGNGYVTRYGHNERI
                     LVKVGDKINKGQRIALMGSTGRSTGPHVHFEVLFNGNVVNPQQYIQAAR"
BASE COUNT         1017 a         2048 c         1909 g         1136 t
ORIGIN      
        1 taaaaaaacg cccgaccgca cctgcttttt cgttgttata aaacataatc ttaaattaat
       61 ttaagatgat aaaaaataac atcgaaaatg gcaatgcggt tggaccgctg gggcgctgcc
      121 cgcagtgctc acagcctgcc cagctgggcg cgcagaatcc ctggcgtccg ttctgctgcg
      181 agcgctgccg ccagctggac ttcggcgcgt ggatcgccga gcgctacacc atcgcggaga
      241 gcggcgaaga gacgccacca gcgcccgcag accatcagct ccagtaggca gagatgccgg
      301 cgacgccctg cgcatagcac gcgtgcgcct gcgccagatc atcaggccct accccgccaa
      361 tcgcgtacac cgccagattg gccttggtgg ccagcagacc gaaatcgctc caccccagcg
      421 ttgcagattg cggatgcgtg gctgtcggca gcaccggccc gagcacggcg aagtcggcac
      481 ccgcgcgccg gagcgcggtg cactcaccgg cgttatgcgc ggaaccgccg aggaggaatg
      541 gttctggcaa ccggcgcgac tcctgcgctg ctacgcgcgc ggcactcagg tgcaggcctg
      601 ccgcgcccac gcgctgcacc agttccacgc tgcgatcgag cattacacgc aagccacggc
      661 cgcgctcgat cacccgctgc gcgagcgtct cgtacgcatc gtcggccagc tgcggcaggc
      721 gcagtcgcag caacgcaccg cgcggcaggc gatccacacc tgcaagcacc tgcccggcgt
      781 tcgcttgcgg cggcgtaaag acatagatgg aaggaagctg caaggcacgc acgatgggcc
      841 catcggcggc gagcaggtcg tgctgatgca gctgctcggg gcggacccac gccacctgct
      901 ggccttcgcg gcagtgcagt tcgccgctcc attgctcgac gcgccaggtg tcgagaacca
      961 ccgtgcgatc ggaatagtca tgggtgatgc ggatcagcgg gcgggcgcgc tgcaccgtga
     1021 tgccgagttc ctcgtgtagt tcgcgcgcca atgcggcacc ggcactttcc ccggcttcga
     1081 tcttgccgcc cgggaattcc catttgcccg ccgcgatctt tcccgacggc cggcgcgcga
     1141 tcagcacggc gccttcggca tcgaccagcg cgccgcaggc caccgggatc accggccgca
     1201 tcaggtgcgg tactcggcgt tgatcttcac gtagtcgtag ctgaaatcgc aggtccacac
     1261 cgtggctgac gcctcgccgc ggcccaatcc tatgcggatg ctgaactcgg gccgcgccac
     1321 caccgccgca ccgagttcct cgcggtaacc gggatgcggc tgcccgccgc gcagcagctg
     1381 cacgtcatcc agagccaggc tgacgccggc cgggtcaagc ccgtgcacgc cggccttgcc
     1441 gatggccatg acgatgcggc cccagttggc gtcgctggcg aacatcgccg tcttgaacag
     1501 cggcgaatgc gccaccgaca acgccacctg ccgcgcctcg gcagtgctgc gcgctccgga
     1561 cacttcgatg gtgatgaact tggtggcgcc ctcgccgtcg cgcacgatgg catgggccag
     1621 ttcacggcac acggcctcga tgccaccggc aattgcggcg aaatccgcgt gcgctggatc
     1681 gatcgcgcgc gtaccgacct tgccggtggc gaacagcatg cacgagtcgt tggtcgaggt
     1741 gtcgccatcc accgtcacgc aattgaagct gtgtgacacg gccgattcca gcgcctgacg
     1801 cgtagcctgt gccgtgaggt gcgcgtccgt gccgatgaac gcgagcatgg tggccatgtc
     1861 tggatggatc atgcccacgc ccttggcgat gccggtcacg gtaaccacgc cctggctggt
     1921 ttcgaccttg cgcgtggcgc ccttgggcac ggtgtcggtg gtcatgatcg ctcgcatggc
     1981 atcgggccag ccgtcggcgc gcaatgcagc acaggccttc ggcgcggcgt ccaccatgcg
     2041 ctgcaccggc aggcgctggc caatcacgcc ggtcgagaat ggcaacacct gctcgggctt
     2101 gcagcccagt gccaacgccg ccgcccggca gctcgccagc gcatcttcga taccgccttc
     2161 gccggtcgca gcgttggcgt tgcccgaatt gatcagcagg ccgcgaatgc cgcccggcgc
     2221 cagctgtttc cggcaaacct gcacgggcgc ggcggcaaaa gcattctgcg tgaagactcc
     2281 tgccacctgc gtgcctgcgt ccagcgcgat gagcagcaga tccttgcggc ccgctttctt
     2341 gatcgcagcc tcggccacgc ccaggcgtac gccagctacg ggatgcagcg tttcaggaac
     2401 ggcgagattg acggccatat cgtgggcagc agcgcgctaa accagcgcac cgtgacattg
     2461 cttgtatttc ttgccgctgc cgcaagggca agggtcgttg cgaccgacct tgggctgctc
     2521 acgcaccacg gttgcgggcc gggccggcgg cgcggcgcgc tgctggagag gccgggccgc
     2581 atccgcaccc ggcggcgcgg gagactcgcc cggcgcggga gcatcaccgg cgaccggcgc
     2641 ggcagcatgc tggaattgca gccggccttc gttcgcctcg gcctgccggc gcctctgggc
     2701 ttcgatttcg gcgacttccg cttcggtctg gacacgcacg cgcgccagca gcgtgatcac
     2761 ctcgtgcttg tagcgggcca gcatgtcatt gaacatgccg aacgcctcgc gcttgtattc
     2821 ctgcttggga tccttctgcg catagccgcg cagatggatg ccttgccgca ggtaatccat
     2881 cgccgccagg tgctcgcgcc agagattgtc gagtacctgc agcatcacgg ccttctcgaa
     2941 atgctcgagc accgcggcac cggcctgctc cttcttctgc gagtaggccg cgcccacccg
     3001 ctcaacgatg cgctcacgca gggctttctc ggtgaggccc ttctcttggt ccagccactg
     3061 cgccaccggc agatcgaggg cgaaatcgcg gcgcagcgcc tcttccaggc ccttggcgtt
     3121 ccattcctgc tcgatcgact gcggcgggat gtaggcgtcg atcaccgcgt tcaccacgtc
     3181 cggccgcagt gcctcgaccc ggtcggaaac gcgttcggct cccatcagct cgtcacgcag
     3241 ctcatagatg gctttgcgct ggtcgttggc gacgttgtcg tattcgagca ggttcttgcg
     3301 gatgtcgaag ttgtggccct cgaccttgcg ctgcgcggtc tcgatggcgc gcgtcaccca
     3361 gcgatgctcg atggcctccc cttcggccat gcccaggcgc tgcagcatcg cgcgcatcga
     3421 cggcggggtg aagatgcgca tgagcgtatc ttcgagtgaa agataaaagc gcgaagcgcc
     3481 cgggtcgccc tggcggccgg agcggccgcg cagctgattg tcgatgcgcc gcgattcatg
     3541 gcgctctgag ccgatcacgt acaaaccgcc ggcggcgacc acggcgtcgt gccgctgctg
     3601 ccacgcgctc cgcgcggctt cgcgcgccac attgtcctct aggggcagcc gggccagttc
     3661 cgtttcgaga ttgccgccaa gtacaatgtc ggtgccgcgg cctgccatat tggtggcgat
     3721 cgtcacggtg cccggcaggc cggcattggc tactatctcc gcctcgcgtt cgtgctgctt
     3781 ggcgttgagc acatcgtgct tgatgccatc cttggccagc aggccggaca gcaattcgga
     3841 agtttcgatg ctggcggtgc cgaccagcac cggctgctgt ttctggtgcg cctgcctgat
     3901 ctcggcaacc accgcctgga acttctcgcg tgcggtcatg aacacctggt cgccggcgtc
     3961 cttgcgcacc atcgcgcggt tgggtggaat caccaccact tccaggttgt agatgttctg
     4021 gaactcgtag gcttcggtat cggccgtgcc ggtcatgccc gacagctttt tgtacagcct
     4081 gaagtagttc tggaacgtga tgctggccag cgtctggttc tcctgctgga tcttcacgtt
     4141 ttctttggct tcgatggcct ggtgcaggcc gtcgctccag cgtcggccgg gcatcatgcg
     4201 cccggtaaac tcgtcgacaa tgatgatctc gccgttgcgc acgatgtact ccacatcgcg
     4261 ccggtacagg tgatgggcgc gcagcaaggc gttcaggtga tgcaccagca cgatgttggc
     4321 cgcgtcgtac aggctgtctt cctcgcccag caggcccgcc tgacgcatga aggtttcaac
     4381 cttttcatgg ccggcttccg acagatgcac ctgcttggct ttctcgtcgg cgtagaaatc
     4441 tccgggcccc tcttcgttgt cctggcgttg cagccgcggc acgaaggcgt tgatcttgcg
     4501 atacagctcg gggtcatcgt cggtcggtcc gctgatgatc agcggcgtgc gtgcttcatc
     4561 gatcaggatc gaatcgactt cgtcgacaat ggcgtagtaa tggccgcgct gcaccttgtc
     4621 agccagcgag aaggccatgt tgtcgcgcag gtaatcgaaa cccagttcat tgttggtggc
     4681 gtaagtgata tcggccgcgt aggcctcgcg cttttcggcc tgcgtctggc cgggaaccac
     4741 gacaccgacg gtcaggccca gaaaacgatg gatacgtccc atccactcgg catcacgctt
     4801 tgccagatag tcgttgacgg tcaccacgtg cacgcccttg gctggcagtg cattcaagta
     4861 ggcgggaagg gtcgagacga gggtcttgcc ctcgccggtc ttcatctcgg caatcttccc
     4921 ttcgtgcagg atggcgccgc cgaccagctg cacgtcgaag tggcgcatgc caaggacacg
     4981 ccggcccgcc tcccgcacca cggcgaaggc ttccgccttc aggctttcca gggcttcccc
     5041 gcctgccagg cgctcgcgga atagctgggt ctggcgtgaa agctcggcgt ccggcaggtc
     5101 gcggaacttc gcctccaggc gaccgacctc cgtgacgaag gaatcgagcc gcttgagtat
     5161 gcgttgattg cggctgccaa aaacccgcgc cagcatgttg ttgaacatag tgataccggg
     5221 ccgcggccag ccgcggcgaa gcacagaaaa agggcgcgta ttatgccctt tgcggcggta
     5281 agacagcgga ttccgctagc gcagaactag cgggcagcct ggatatactg ctgcggattg
     5341 acgacgttgc cgttgaacaa cacttcaaag tgcacgtgcg gacccgtgct gcggccggtc
     5401 gaccccatca gggcgatacg ctggcccttg ttgatcttgt cgcccacctt gaccaagatg
     5461 cgctcgttgt gaccatagcg ggtcacgtaa ccattcccat ggttgatttc aaccaggttg
     5521 ccgtaaccga aacgctcacc ggcttcggtg acgatgccgg tcgccaccgc gagcacatct
     5581 gctccctcgc gcgcagcgaa atcaatgccg gtgtgctggg catagcggcc ggtgaaagga
     5641 tccgtacgcc agccaaaggt tgaagaaatc cagccattgt cgaccggcca cccggatggc
     5701 ttcacctgtt tttgcagacg gctggccagc agcaggtcct cgagcacacg catctggcgc
     5761 tctcgatccg acaactgcgc ctcgaattga tcgagcgaag atataacctg taccagcccg
     5821 gtttcctggg cggatgcctc ttcgggaccg cccaccgcag gagcctgatc gaagttgaat
     5881 tcgccgcgat ccagcccggc gatttccgtc agccgcgcgc cggcggcatt gactcgcatc
     5941 atgtgagcat gaagctgggc aatccgacgg gccaaggccc gggcattctc ctcggcttcc
     6001 tcccgcgcct tgttcagttc gctgtgctgg gcgctgactt cctccgccca tgtggccacc
     6061 aatccgctgg gcagcacact gccatcgccg cgcgcaaggt agcccagccc
//
LOCUS       DASZZC010000321         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_991020,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000321 DASZZC010000000
VERSION     DASZZC010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991020"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VHE37_06735"
     CDS             complement(<1..1495)
                     /locus_tag="VHE37_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVT35259.1"
                     /translation="MSKTLNPVAWGHVEDALNDKERKPLLALLGGFLRGAKLDAVPPP
                     VAADVSLPPSRVEIPSAMSAFVTAAHDARVLHAVGRSFRDLATLRTAALAHAPDVVAT
                     PRSGQELLDVLGWADAARYAVIPYGGGSSVVGGINAEGMDGYNGVVTLSLRALDRVLE
                     VDARSAVVHAQAGILGPDLDAALKPHGLAVRHYPQSYLHSTLGGWVATRGAGHFSTQL
                     AKIEDRVEALSVMLPDGRRVQTRALPASSVGVDPNRLWCGSEGALGVITDVRLRCVRL
                     PTQRLSAGVRFKTYEQALEAARELVQGGILPTQLRILDPYEHMLSAAYSGKAAQGALM
                     VLGFESAGAPLAETHAAALALTQKMGGEIKDDGGREAVGDWRSTFFRQPYLRDALLDY
                     AVISDTFETAVPWSRLPQFYHAVREATARAVTQECGAGMVSCRTTHAYSDGCSLYFAF
                     FGAGKRAELVEQWWRIKRAASAAVMACGGTISHHHAMGRDHRHWAREE"
     gene            complement(1492..>2193)
                     /locus_tag="VHE37_06740"
     CDS             complement(1492..>2193)
                     /locus_tag="VHE37_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVT35260.1"
                     /translation="LDSYHQLAEAYGQLDLDHELRCGVVYGKGEHFTAGLDLPQWTDT
                     LGNGRWPALGAKQRDPFRLDAARQLSKPLVFAAQGICFTVAIELMLAGDIRVAADNCR
                     FGQIEVRRGIYACGGATVRMVQEFGWANAQRYLLTGDEFNAAEALRIGLVQEVVPLGR
                     QLERAVELAEKIAKQAPLGVYGSLRSSRIAVEEGVRAAIDRFLPDLTPIMKSHDVIEG
                     VQSFVERREAKFQGK"
BASE COUNT          346 a          803 c          724 g          320 t
ORIGIN      
        1 tttcctcgcg tgcccaatgg cggtgatcgc gccccatcgc atggtggtgg ctgatggtgc
       61 cgccgcacgc catcaccgca gccgacgcgg cgcgcttgat gcgccaccac tgctcgacca
      121 gttctgcgcg cttgccggcg ccgaagaagg cgaagtacag cgagcagccg tctgaataag
      181 cgtgcgtggt gcggcacgag accatgccgg cgccgcactc ctgcgtcacc gcccgcgccg
      241 ttgcctcgcg caccgcgtgg tagaactgcg gcaggcgcga ccatggcacc gccgtctcga
      301 acgtatccga gatcaccgcg taatcgagca gcgcgtcgcg caggtacggc tggcggaaga
      361 aggtgctgcg ccagtcgccc accgcttcgc gcccgccgtc gtccttgatc tcgccaccca
      421 tcttctgcgt gagcgcgagt gccgccgcat gcgtctccgc cagcggcgcg ccggccgatt
      481 caaaacccag caccatcagc gcgccctgcg cggccttgcc ggaatatgcg gcgctgagca
      541 tgtgctcgta cgggtcgagg atgcgcagct gcgtcggcaa tattccgccc tgcaccagtt
      601 cgcgcgccgc ctccagcgcc tgctcgtagg tcttgaaacg cacgccggcg gacaaccgtt
      661 gcgtcggcaa ccgcacgcag cgcaggcgca cgtcggtgat cacgcccagc gcgccctcgc
      721 tgccgcacca caggcggttg ggatcgacac ccacgctcga cgccggcaat gcgcgcgtct
      781 gcacgcggcg cccatccggc aacatcaccg agagcgcctc gacgcgatcc tcgatcttgg
      841 ccagctgcgt ggagaaatgc ccggcgccgc gcgtggccac ccagccgccg agcgtggagt
      901 gcaggtacga ctgcgggtaa tggcgcaccg ccagtccgtg cggcttgagc gcggcatcga
      961 gatcggggcc caggatgccg gcctgcgcgt gcaccaccgc gctgcgcgca tcaacttcca
     1021 gcacgcgatc cagcgcgcgc agcgacagcg tgacgacgcc gttatagccg tccatgcctt
     1081 cggcgttgat gccgccgacc acgctggaac cgccgccgta gggaatcact gcatagcgcg
     1141 cggcatcggc ccagccgagc acatcgagca gttcctgccc gctgcgcggc gtggccacca
     1201 cgtcaggggc gtgcgccagc gccgcagtac gcagcgtggc caggtcgcgg aagctgcggc
     1261 cgacggcgtg cagcacgcgc gcgtcgtgtg cggccgtcac gaaagccgac atcgcggaag
     1321 gtatttccac gcgtgaaggc ggcaacgaga catccgcggc caccggtgga gggaccgcgt
     1381 cgagcttcgc cccgcgcagg aaaccgccga gcaaggccag cagcggcttg cgctccttgt
     1441 cgttcagcgc atcttcgaca tgaccccagg caaccgggtt gagcgtcttg ctcacttgcc
     1501 ctggaacttc gcttcgcgcc gctcgacgaa cgactgcacg ccctcgatca cgtcatggct
     1561 cttcatgatc ggcgtgaggt ccggcaggaa gcggtcgatg gccgcgcgca cgccctcctc
     1621 caccgcgatg cgcgacgagc gcaggctgcc gtacacaccc agcggcgcct gcttcgcgat
     1681 tttttccgcc agctccaccg cccgctcaag ctggcggccc agcggcacca cctcctgcac
     1741 caggccgatg cgcagcgcct cggctgcgtt gaactcgtcg ccggtcagca ggtagcgctg
     1801 cgcgttggcc cagccgaact cctgcaccat gcgcacggtg gcgccgccgc aggcgtagat
     1861 gccgcggcgc acctcgatct ggccgaagcg gcagttgtcg gcggcgacgc ggatgtcgcc
     1921 ggccagcatc agctcaatgg cgacggtgaa gcagatgccc tgcgcggcga acaccagcgg
     1981 cttggacaac tgccgcgcgg cgtcgaggcg gaacggatcg cgctgcttcg cgcccagcgc
     2041 cggccagcgg ccattgccca gcgtgtcggt ccactgcggc aggtcgaggc cggcggtgaa
     2101 atgctcgccc ttgccgtaga ccaccccgca gcgcagctcg tgatcgaggt cgagctgacc
     2161 gtaggcctcg gccagctggt gatagctgtc gag
//
LOCUS       DASZZC010000322         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1120965,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000322 DASZZC010000000
VERSION     DASZZC010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120965"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VHE37_06745"
     CDS             <1..361
                     /locus_tag="VHE37_06745"
                     /inference="COORDINATES: protein motif:HMM:NF023592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HVT35261.1"
                     /translation="RIDAMPLEGFVRQLARNCAWLGREGDTVKLALDAKVRHLLQEDR
                     RASLERSLAGQLNSPVKLLIEFSSEAATPARLEQQRVSEQQRAAESAIEADPTVRAFK
                     DTFGASVKAGSVQPTGK"
     gene            513..836
                     /locus_tag="VHE37_06750"
     CDS             513..836
                     /locus_tag="VHE37_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HVT35262.1"
                     /translation="MRPELGNLMRQAQKMQENLKRVQEEIAQLEITGEAGAGMVKVTI
                     NGRHEARKVEVNPEALKEDKEFLEDLLAAAINDAAHKLEQASKEKMAKVTGGMQLPPG
                     MNLGF"
     gene            919..1518
                     /gene="recR"
                     /locus_tag="VHE37_06755"
     CDS             919..1518
                     /gene="recR"
                     /locus_tag="VHE37_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HVT35263.1"
                     /translation="MSNYSPRLTRLIEALRRLPGVGPKSAQRMAFHLLDRDRSGAEYL
                     GKAIGEALSGITRCPSCRMFCEDGACRYCTPQRRESGQLCVVESPADLMAIEHGTQFR
                     GRYFVLMGRLSPLDGVGAAQLGLDTLAQQLAAGEVKEMIIATNPTVEGEATAYYLAEM
                     ARKQNIRVTRIAHGVPMGGELEYVDGGTLSHALSGRQPV"
     gene            1713..1973
                     /locus_tag="VHE37_06760"
     CDS             1713..1973
                     /locus_tag="VHE37_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35264.1"
                     /translation="MPRGIVSEEKLVAQLAKVEEKRRVLRKKLQVFRIRAAAKKRKAV
                     FSSIRKNEKEVMEVLKNKAPDFFKKLLTAQPAKRGRRKKAAG"
     gene            complement(1977..2312)
                     /locus_tag="VHE37_06765"
     CDS             complement(1977..2312)
                     /locus_tag="VHE37_06765"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HVT35265.1"
                     /translation="MNELAKKKCVPCEGKVPPLTAEAAGQLKQQLRAEWKLLEQGKKL
                     QASFSFSNYYRTTAFVNAVAWIAHSEDHHPDISFGYNSCSVLYWTHSINGLSENDFIC
                     AAKVDALLG"
     gene            complement(2317..>3188)
                     /locus_tag="VHE37_06770"
     CDS             complement(2317..>3188)
                     /locus_tag="VHE37_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT35266.1"
                     /translation="SDDMYGGSYRLFARVRERSAGLKFSFVDMRDAGNIERAITPATR
                     LIWVETPTNPMLKIADLSAIGRLARRHRLIAAADNTFASPALQRPIEHGFHVVMHSAT
                     KYLSGHSDMVGGMLVVGAHPDLEEKIAFLQNAVGAVAGPFDSFLALRGVKTLALRVQR
                     HSENALALARWLEKHPKVARVHYPGLLSHAQHAQARRQMRGGYGGMISIELKGTLKTA
                     RRFLERCEIFALAESLGGVESLIEHPAIMTHASVPAATRRKLGISDTLCRLSVGVEDL
                     QDLRSDLDQALRG"
BASE COUNT          626 a          976 c         1050 g          536 t
ORIGIN      
        1 gcgcatcgat gccatgccgc tggaaggttt cgtgcgccag ctggcgcgca actgcgcctg
       61 gctcggccgc gaaggcgaca cggtgaagct cgcgctcgat gccaaggtgc gccacctgct
      121 gcaggaagac cgccgcgcct cgctcgaacg cagtcttgcg gggcagctga actcgccggt
      181 gaagctgctg atcgaattct ccagcgaagc tgcgacgccg gcgcgcctgg agcagcagcg
      241 cgtgagcgaa cagcagcgcg cggcggagag cgccatcgaa gccgatccca ccgtgcgcgc
      301 gttcaaggac accttcggcg ccagcgtcaa ggcgggatca gtgcagccca cgggcaagtg
      361 agcgcgcgtt tccagcagat ttttctatta ccgcatcggc gattttgatg ttatttatta
      421 ttgtattaag ttaaaaacag acaataaata gtaacaagct aaggcggcat gcggttggct
      481 tttttctggg acgaaatacg gatggaacag tcatgagacc tgaactcggg aacctgatgc
      541 gccaggcgca gaagatgcaa gaaaacctga agcgcgtgca ggaagaaatc gcgcaactgg
      601 agatcaccgg cgaagcgggc gcgggcatgg tcaaggtcac catcaacggc cggcatgaag
      661 cgcgcaaggt ggaggtgaat cccgaggcgc tgaaggaaga caaggaattc ctcgaagacc
      721 tgctggcggc ggcgatcaac gatgccgcgc acaagctcga gcaggcgagc aaggagaaga
      781 tggccaaggt caccggcggc atgcagctgc cgcccggaat gaacctgggc ttctgaaagg
      841 ctttttgtag gagcgggccc tgcccgcgat acaccgctgc aatcgcgcgc caggcgcgct
      901 cctgcaggaa aattttccat gagcaactat tcgccaaggc tcacacgttt gatcgaggcg
      961 ctgcgccggc tgcccggcgt ggggccgaag tcggcgcagc gcatggcctt ccatttgctc
     1021 gaccgcgacc gcagcggcgc ggaatacctg ggcaaggcca tcggcgaggc gctgagcggc
     1081 atcacgcgct gcccgagctg ccgcatgttc tgcgaggacg gcgcctgccg ctactgcacg
     1141 ccgcaacggc gcgagagcgg gcagctgtgc gtggtggaat cgccggccga cctgatggcg
     1201 atcgagcacg gcacgcagtt ccgcggccgc tacttcgtgc tgatgggccg gctttcgccg
     1261 ctggatggcg tcggcgcggc gcaactgggc ctggacacac tggcgcagca gctggcggcg
     1321 ggcgaggtga aggaaatgat catcgccacc aatcccacgg tggaagggga ggccacggcc
     1381 tattacctgg cggagatggc gcgcaagcag aacatccgcg tgacccgcat tgcgcatggc
     1441 gtgccgatgg gcggtgaact cgaatacgtg gacggcggca cactcagcca tgccctcagc
     1501 gggcgtcagc ccgtctgaaa gcaaagatgc gctgaaggcg cacgaaatcc cctctcccgc
     1561 gtgcgcgaga ggtcagggtg aggagcggtc gcagaccgct agtggccgcc agcccgtctg
     1621 agaaataacg cggcttgagg taatgcggcg gccaggcaat accggcacaa ccacaacggt
     1681 gtaaactttg ctatcacttg aaggagtcat ccatgcctag aggcatcgtc tcggaagaaa
     1741 aactggtcgc ccagctggcc aaggtcgaag agaagcgccg cgtgctgcgc aagaaacttc
     1801 aggtattccg catccgtgct gcagccaaga agaggaaagc ggttttctcc tcgatccgca
     1861 agaacgagaa ggaagtgatg gaagtgctga agaacaaggc accggacttc ttcaagaagc
     1921 tgctcactgc acagccggcc aagcgcgggc gccgcaagaa ggccgccggc tgagagtcat
     1981 cccagcagcg cgtcgacctt cgccgcgcag atgaagtcgt tttccgacag tccgttgatg
     2041 ctgtgcgtcc agtacagcac gctgcagctg ttgtagccga aactgatgtc ggggtgatga
     2101 tcttcgctgt gcgcaatcca ggcgacggca ttcacgaatg ccgtagtccg gtagtagttg
     2161 gagaaggaaa agctggcctg cagttttttt ccctgctcca gcagtttcca ctcggcgcgc
     2221 agctgctgct tcagctggcc ggcggcttcc gccgtcagcg gtggcacctt gccctcgcag
     2281 ggcacacatt tcttttttgc cagttcattc atcgtttcat cctcgcaggg cctgatccag
     2341 gtcagaccgc aggtcttgca ggtcttccac gccaaccgac aggcggcata gcgtatcgct
     2401 gatgccgagt ttgcgccggg tggccgccgg cacgctggcg tgggtcatga tggccgggtg
     2461 ctcgatcagg ctctccacgc cgcccaggct ctcggccagc gcgaagattt cacagcgttc
     2521 aagaaagcgc cgcgcggttt tcagcgtgcc tttcagctcg atggagatca tgccgccata
     2581 accgccgcgc atctgccggc gcgcctgcgc gtgctgcgca tgcgagagca ggccggggta
     2641 gtgcacgcgc gccactttgg gatgcttctc cagccagcgc gccagcgcca gcgcgttctc
     2701 gctgtggcgc tgcacgcgca gcgccagcgt cttcaccccg cgcagggcga ggaacgagtc
     2761 gaacgggccg gccaccgcgc ccaccgcgtt ctgcaggaag gcgatcttct cctccaggtc
     2821 aggatgggcc cccaccacca gcatgccgcc gaccatgtcg gaatggccgg aaagatattt
     2881 cgtcgccgag tgcatcacca cgtggaagcc atgctcgatc ggccgctgca gcgccgggct
     2941 ggcgaaggtg ttgtcggctg cggcgatgag ccggtgcctg cgcgccagcc ggccgatggc
     3001 cgacaggtcg gcgatcttga gcatcgggtt ggtcggcgtc tccacccaga tcaggcgcgt
     3061 ggccggcgtg atggcgcgct cgatgttgcc cgcgtcgcgc atgtcgacaa agctgaactt
     3121 gagcccggcc gagcgctcgc gcacgcgtgc aaacaggcga tagctgccgc cgtacatgtc
     3181 gtcgctgg
//
LOCUS       DASZZC010000323         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1245638,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000323 DASZZC010000000
VERSION     DASZZC010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245638"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /gene="lysA"
                     /locus_tag="VHE37_06775"
     CDS             complement(<1..963)
                     /gene="lysA"
                     /locus_tag="VHE37_06775"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HVT35267.1"
                     /translation="MDHFNYRNGELYAEDVPVREIAARHGTPCYVYSRATLTRHFKAF
                     DDALAGTDHLVCYAVKANSNLAVLQVLARLGAGFDIVSGGEMQRVLRAGGDARRIVFS
                     GVGKTESEIRAALAAGIYCFNVESETELRLLDRIGSELQRRAPIALRVNPDVDAKTHP
                     YISTGLKNNKFGVDIATAERLYLAARDMPGVEVLGVASHIGSQLMDVQPLLDAVDRML
                     ALIERLRAQGVSFRHIDVGGGLGVRYHEEQPPHPSEWSRALRPKLEGRGLKILTEPGR
                     AIAGNAGILLTRVLALKLAEAKNFAVIDAAMNDLIRPTLSQAWMD"
     gene            complement(982..1149)
                     /locus_tag="VHE37_06780"
     CDS             complement(982..1149)
                     /locus_tag="VHE37_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HVT35268.1"
                     /translation="MKFVLSFVVLLLCACGQAGALYLPGQSPQQGKREQQAAPTAPPQ
                     ATPATSSGSSP"
     gene            complement(1256..1861)
                     /locus_tag="VHE37_06785"
     CDS             complement(1256..1861)
                     /locus_tag="VHE37_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35269.1"
                     /translation="MSSRFGFWAAWAAAIAAIAYDIPQLLQVFGAIREPLDRILIFAP
                     SLALAPSFVLAVVAVHASTSADRRVWSLGALAIALLYAADVSQVYIIQLGAVIPRDIA
                     GDHSLASAACCGPQLPMTSLDLLGYTYMSLSTLLLVPVFGNVASMRALRLALALNGCL
                     APVIFGQLLLPSLIYVAAAWLVTFPACMILLAQSFFARSEK"
     gene            2095..2763
                     /locus_tag="VHE37_06790"
     CDS             2095..2763
                     /locus_tag="VHE37_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVT35270.1"
                     /translation="MSEDDDVIVIDPPARASAAVIWLHGLGASGDDFVPIVPELGLPA
                     GHGIRFVFPHAPVRAVTINGGMQMRAWYDITGLSRTALADRAGIEASQQLVEQLIAQQ
                     RAAGIASEKIVIAGFSQGGAIALHTATRHAEPLGGILALSTYLPLHESLAVEAASANR
                     KIPIFMCHGTYDPVLPYALGEFSCAQLRAHGYEVDWRSYPMQHQVCMEEIEDIGRWLL
                     QRLA"
BASE COUNT          525 a          912 c          929 g          457 t
ORIGIN      
        1 gtccatccag gcctgggaca gcgtgggccg gatcaggtcg ttcatcgccg cgtcgatcac
       61 cgcgaaattt ttcgcctccg ccagcttcag cgccagcacg cgcgtgagca ggattcccgc
      121 gttgccggcg atggcgcggc ccggctcggt gaggatcttc agcccgcgcc cttcaagctt
      181 cggccgcagc gcacgcgacc actcgctcgg atgcggcggc tgctcttcgt ggtagcgcac
      241 gcccagcccg ccgcccacat cgatatggcg gaaagagacg ccctgcgcgc gcaggcgctc
      301 gatcagcgcc agcatgcggt cgaccgcgtc gagcagcggc tgcacgtcca tcagctgcga
      361 gccgatgtgg ctggccacgc ccagcacttc cacgccgggc atgtcgcgcg cagcgagata
      421 caggcgctcg gcggtggcga tgtcgacgcc gaacttgttg ttcttcagcc cggtggaaat
      481 gtatgggtgc gtcttcgcgt ccacatcggg attcacccgc agcgcaatgg gcgcacgccg
      541 ctgcaactcg ctgccgatgc gatcgagcag gcgcagctcg gtttccgatt ccacgttgaa
      601 gcaatagatg ccggcggcca gcgccgcgcg gatttcgctc tcggtcttgc ccacgccgga
      661 gaacacgatg cgccgcgcgt caccacccgc acgcagcacg cgctgcatct cgccgccgga
      721 gacgatgtcg aagccagcac cgagccgcgc cagcacctgc agcaccgcga gattggagtt
      781 ggccttcacc gcgtagcaca ccaggtgatc ggtgccggcc agcgcgtcgt cgaaagcctt
      841 gaaatggcgc gtcagcgtgg cgcgcgaata gacgtagcac ggcgtgccgt ggcgcgcggc
      901 gatctcgcgc accggcacgt cttcggcgta gagctcgccg ttgcgatagt tgaagtggtc
      961 cacggaggga tcgaaaaaga tttaagggct gctgccggaa ctggtggcgg gcgtcgcctg
     1021 cggcggggcc gtgggcgccg cctgttgctc gcgtttgccc tgctgcggcg actggcccgg
     1081 caggtacagc gcgccggcct ggccgcaggc acacagcaga agaacgacga acgacaggac
     1141 gaatttcatg gggcgcaaag tgtagcccga atattgaacg ggtcagcgac gttggagcgg
     1201 aggtgaactg cacgctccgg tggcggtcac ggctttgtgc caggagccgg tgaggtcact
     1261 tctccgatcg tgcaaagaat gactgtgcga gcaggatcat gcaggcggga aaggtgacga
     1321 gccatgcggc agcaacgtag atcaggcttg gcagcagcaa ctgcccgaag atcaccggcg
     1381 ccaggcatcc gttgagtgcc aaggccagac ggagtgcacg catggacgcg acattgccga
     1441 acacggggac gagcagcagc gtcgacaggc tcatgtaggt gtatccgagc aagtcgagcg
     1501 acgtcattgg cagctgggga ccgcaacagg cggcgctcgc caggctgtgg tctcccgcaa
     1561 tgtcgcgtgg gatcactgcg ccaagctgaa tgatgtaaac ctgactgaca tcggctgcgt
     1621 acagcaaagc gattgccagt gcaccaaggc tccagacgcg gcgatctgcg ctggtggacg
     1681 cgtgcacggc aacgacggcg agaacgaaac tgggcgcgag tgccagcgaa ggtgcaaaga
     1741 tcaggatgcg atccagcggc tcgcggattg cgccaaagac ttgcagcaac tgcgggatgt
     1801 cgtacgcaat ggctgcgatt gccgcggccc aggccgccca gaagccaaat cgtgaactca
     1861 tctgaatgcg cccaacaccg gttgcccgtc tgcaccgcga ctggcaaaag cctggcggac
     1921 tgaaccagta gcggcattga cctgcgtcat acgatgagcc atcgagcggc agatccgcca
     1981 atcaaccgaa aaggttgggc cccgattgct tgaagcccgc gcgttggtcc aggccactgc
     2041 aagatttttt caacgccacg cggccgtatc cgcaactcct gcgacaatgc gcgcatgagt
     2101 gaagacgatg atgtgatcgt gatcgacccg cctgcgcgcg ccagcgccgc ggtcatctgg
     2161 ctgcacggcc tgggcgccag cggcgacgac ttcgtgccga tcgtgccaga gctcgggctg
     2221 cctgccggcc acggcatccg cttcgtgttt ccgcacgcgc cggtgcgcgc agtgaccatc
     2281 aacggcggca tgcagatgcg cgcgtggtac gacatcaccg gcctgtcgcg caccgcgctg
     2341 gccgaccgcg ccggcatcga ggcttcgcag caactggtgg agcaactgat cgcgcagcag
     2401 cgcgcggcag gcatcgccag cgaaaaaatc gtcatcgccg gcttctcgca gggcggcgcc
     2461 atcgcgctgc acacggccac gcgccatgcc gagccgcttg gcggcatcct cgcgctgtcc
     2521 acctacctgc cgctgcacga gagcctggcg gtggaagccg catcggccaa ccggaaaatc
     2581 cccatcttca tgtgccacgg cacctacgac ccggtgctgc cgtacgcgct cggcgaattt
     2641 tcctgcgcgc agctgcgcgc gcatggctac gaggtggact ggcgcagcta cccgatgcag
     2701 caccaggtgt gcatggaaga gatcgaggac atcggccgct ggctgctgca gcggctggcc
     2761 tgagcgcctt ttttgctcca ccgcaccggc acttttgatg ttatgaaata ttgtcttatt
     2821 tta
//
LOCUS       DASZZC010000324         5019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_603116,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000324 DASZZC010000000
VERSION     DASZZC010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603116"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..363
                     /locus_tag="VHE37_06795"
     CDS             151..363
                     /locus_tag="VHE37_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVT35271.1"
                     /translation="MTTATTGTVKWFNETKGFGFITPDGGGEDLFAHFREIQGSGFKT
                     LKEGQKVEFKPQRGPKGMQATQIRSL"
     gene            462..671
                     /locus_tag="VHE37_06800"
     CDS             462..671
                     /locus_tag="VHE37_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVT35272.1"
                     /translation="MANATGTVKWFDDAKGYGFITPTDGSGDLFAHFKEIQGSGFKSL
                     KEGQKVEYEPQKGPKGMQATKIRPL"
     gene            817..1971
                     /locus_tag="VHE37_06805"
     CDS             817..1971
                     /locus_tag="VHE37_06805"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HVT35273.1"
                     /translation="MSVTEADRAASPVNLPAAIMFSLTTLGVLTVLPWYFWNFDLHAS
                     AWAWFAMLMVANGMAITAGYHRLWAHRTYEAHWSLQILYMLVGAMALQNSALTWCSQH
                     RIHHKHVDDVDHDPYSIKRGFWYAHIGWMLRDYPSGKLDFANAKDLEANPIVMFQHRH
                     YLGIALFMNIAFPLLLGFAYGDPWGFLMLGGLLRLVASHHVTFCINSLAHYWGRRPYT
                     SENSARDNDLIALFTYGEGYHNFHHLFQYDYRNGVRWWQIDPTKWWIAACSWFGLTRS
                     LKRVPEFKIQRAMLQRQFEKAQEKLAALRDRASPTRLSLLQEQLEKEMEAFSATVNAW
                     SQLQAEKFEAARKELADHWEHSDMRRRLQALEDALREQRERISSLHLQAA"
     gene            complement(2013..2255)
                     /locus_tag="VHE37_06810"
     CDS             complement(2013..2255)
                     /locus_tag="VHE37_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35274.1"
                     /translation="MNAKEKLMAARAKAADAALTWVLAHQDRVDALRRSLKGTRADGV
                     IDRLLALLRGAAGRSKPAKRAAKKKTAKRAVRKRAK"
     gene            complement(2295..2708)
                     /gene="arfB"
                     /locus_tag="VHE37_06815"
     CDS             complement(2295..2708)
                     /gene="arfB"
                     /locus_tag="VHE37_06815"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HVT35275.1"
                     /translation="MFQITSTLAIDETEIGLSAMRSGGPGGQHVNKTESAVQLRFDVR
                     ASSLPEPVKQRLLAARDQRISADGVVIIKAQQARSQAQNRADALERLRQLIECASHVP
                     RVRRATRPSRSSQRKRVDRKVQHGRLKALRSRVDD"
     gene            2730..3971
                     /gene="kynU"
                     /locus_tag="VHE37_06820"
     CDS             2730..3971
                     /gene="kynU"
                     /locus_tag="VHE37_06820"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HVT35276.1"
                     /translation="MGLLSRKDCVALDQADPLRGLRERFVLPEGLIYLDGNSLGAMPC
                     AVPARIAQLLEREWGEGLVRSWNTANWVDLPQRLGDKIGRIIGAQPGETLVADSTSVN
                     LYKLLAMALSLRPQRRVILSERENFPTDLYIAQGLIESLGARHELRTVAPQQIESALD
                     DSVAVLMLGHINYRSGAMHDLARLTGRAHAAGALVLWDLAHSAGAVPLRLAADGADFA
                     VGCGYKFLNGGPGAPAFLYIAQRWHGEVRTPISGWFGHARPFEFLPGYEAAPGIARAL
                     AGTPPIISMAALEAGVELALEVSIEQLRAKSMRQVELLAALAAQELAEFGFTDITPRE
                     AQQRGSQLSLMHAQAWPITQALAARNVIGDFRTPDVLRLGVTPMYLGYAELWDAICAL
                     RQVMVAEEWRQPQHQQRTRVT"
     gene            3973..>5019
                     /locus_tag="VHE37_06825"
     CDS             3973..>5019
                     /locus_tag="VHE37_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVT35277.1"
                     /translation="MGDATQAAATGWFRNLPIGRKLAAVVIAYVSIIAVLLLVVRASL
                     DITSGVRAYVGGEGLWSKGQKDAVYYLTRYARSHDQHDYLKYREALAVPLGDGVARRE
                     LLKPAFDHDTAARGFIEGANDAADVDYMIDLFRRFRRVSYMAQAIDIWTRAEAGIIEM
                     QTAGDELHGAVAAGTLTPDEETAIIGRIETINRRLTPLEHDFSATLGEAARWIRQVLQ
                     AVIVLASILLLVAGLAVSLRVTRMLRGDIEQLRRGAARVAAGDLSQPVPPGARDELGE
                     LAAAFNDMIEHRRGAEEALRSANDFRQKVMESATNAIYALDLQGRFTLINRRTCEITG
                     RSEAELLGHHFGMLF"
BASE COUNT          854 a         1667 c         1653 g          845 t
ORIGIN      
        1 cgcagtcgtt tcaaggtgtt gtaccgcggt cccgtttacc cgtcttccca aggcggcgtg
       61 tcgcaggtcc ctccggctac ctttcttgat cgcttgcaca tgcgggcgca gtgtccgcac
      121 aacatttttt tcaataaagg acagttttaa atgactacag cgacaaccgg caccgttaaa
      181 tggtttaacg aaacgaaagg attcggcttc attactccgg atggcggtgg cgaagacctc
      241 ttcgcgcact tccgcgagat ccagggttcg ggcttcaaga ccctgaagga aggccagaag
      301 gtggagttca agcctcagcg cggccccaag ggcatgcagg cgacgcagat tcgctcgctg
      361 taagtcagag gtagcaagct tcaagcttca aaagaaaccg cggtgatacc caccgcggtt
      421 tttttgtttt tggtacaacc acaacataaa gggaatcgcg catggcaaat gcaaccggca
      481 ccgtgaaatg gttcgatgac gccaaggggt atggcttcat cacccccacc gacggcagcg
      541 gcgacctgtt cgcccacttc aaggaaatcc agggctccgg cttcaagagc ctgaaggaag
      601 gccagaaggt ggaatacgaa ccgcagaagg gccccaaggg catgcaggcg accaagatcc
      661 gcccgctgta agcattgccg cgcggccacg cccgcaaggg cgtggcccgg ccggacatac
      721 gtcaaaaaaa ccatcccggc ctgcggtagg cgttgttatg attccattga cccggcggat
      781 ttgccgggcg tgcgattctg cgaggggaat ctccacatga gcgtcaccga agcggaccgg
      841 gctgccagtc cggtcaacct gcctgccgcc atcatgttca gcctgaccac gctgggcgtg
      901 ctgaccgtgc tgccgtggta cttctggaac ttcgacctgc atgccagcgc ctgggcctgg
      961 ttcgccatgc tgatggtcgc caacggcatg gccatcaccg ccggctatca ccggctgtgg
     1021 gcgcaccgca cctacgaggc gcactggagc ctgcagatcc tgtacatgct ggtcggcgcc
     1081 atggcgctgc agaacagcgc gctcacctgg tgctcgcagc accgcatcca ccacaagcac
     1141 gtcgatgacg tggaccacga tccgtactcg atcaagcgcg gcttctggta cgcgcacatc
     1201 ggctggatgc tgcgcgacta tccgagcggc aagctggatt tcgccaatgc caaggacctg
     1261 gaagccaatc ccatcgtgat gttccagcac cggcattacc tgggcatcgc cctgttcatg
     1321 aacatcgcct tcccgctgct gctcggcttc gcctacggcg acccgtgggg cttcctcatg
     1381 ctgggcggcc tgctgcgcct ggtggccagc caccacgtga ccttctgcat caactcgctg
     1441 gcgcactact ggggccgccg cccctacacc agcgagaaca gcgcgcgcga caacgacctg
     1501 atcgcgctgt tcacctacgg cgagggctac cacaacttcc accacctgtt ccagtacgac
     1561 taccgcaacg gcgtgcgctg gtggcagatc gatccgacca agtggtggat tgccgcctgc
     1621 tcctggttcg gcctgacccg cagcctcaag cgcgtgccgg agttcaagat ccagcgcgcc
     1681 atgctgcagc gccagtttga gaaggcgcag gaaaagctgg ccgccctgcg cgaccgcgcc
     1741 agtcccacgc gtctgtcgct gctgcaggag cagctggaga aggagatgga ggcgttcagc
     1801 gccacggtca acgcctggag ccagctgcag gcggagaagt tcgaagccgc gcgcaaggaa
     1861 ctggccgacc actgggagca cagcgacatg cgccggcgcc tgcaggcgct ggaggacgcg
     1921 ctgcgcgaac agcgcgagcg catcagctca ctgcacctgc aggcagcctg agcgggaatc
     1981 gatgcaagaa cgccggccgc gagccggcgt ttttatttgg cgcgcttgcg taccgcgcgc
     2041 ttcgctgtct tcttcttcgc ggcgcgcttc gccggcttgc tgcgcccggc ggcgccgcgc
     2101 agcagcgcca gcagccggtc gatcacgcca tccgcgcgcg tgcccttgag cgagcggcgc
     2161 agcgcatcga cacgatcctg gtgcgccagc acccaggtca gggcggcgtc ggcggccttg
     2221 gcgcgcgcgg ccatcagttt ttcttttgcg ttcatgaatc ctccatgcaa tgaagcggcg
     2281 cgagcatacc cgctttaatc gtcgacgcgc gaccgcaagg ccttgagccg tccgtgctgg
     2341 accttgcgat ccacgcgctt gcgttgcgag ctgcgcgaag gccgggtggc gcggcgcacg
     2401 cgcggcacgt gactggcgca ctcgatcagc tggcgcagcc gctcgagcgc gtcggcgcga
     2461 ttctgcgcct ggctgcgcgc ctgctgtgcc ttgatgatga ccacgccgtc ggcgctgatg
     2521 cgctggtcgc gcgcggccag caggcgctgc ttcaccggtt ccggcagcga tgaggcgcgc
     2581 acgtcgaagc gcagctgcac cgcgctctcg gtcttgttga catgctggcc gccgggcccg
     2641 ccgctgcgca tggccgacag cccgatttcc gtttcatcga tggccagcgt ggaggtgatc
     2701 tggaacatgt gctgcatcat acttcgggca tgggactgct gagccgcaag gattgcgtcg
     2761 cgctggacca ggccgatccg ctgcgcggcc tgcgcgagcg tttcgtgctg ccggaaggcc
     2821 tgatctatct cgacggtaat tcgctgggcg ccatgccgtg cgcggtgccg gcgcgcattg
     2881 cgcagctgct cgagcgcgaa tggggcgagg gcctggtgcg cagctggaac acggcgaact
     2941 gggtcgacct gccgcagcgc ctgggcgaca agatcggccg catcatcggc gcgcagccgg
     3001 gcgaaacgct ggtggccgac tccacctcgg tcaacctgta caagctgctg gccatggcgc
     3061 tgtcgctgcg tccgcagcgc cgcgtgatcc tgtccgagcg ggaaaatttt cccaccgacc
     3121 tgtatatcgc gcagggcttg atcgaatcgc tgggcgcgcg ccacgagctc aggacggtag
     3181 cgccgcagca gatcgaatca gcgctcgacg acagcgtcgc cgtgctgatg ctcggccaca
     3241 tcaactaccg cagcggcgcg atgcatgacc tggcgcggct taccgggcgt gcgcacgcag
     3301 ccggcgcgct ggtgctgtgg gacctggcgc attccgccgg cgcggtgccg ttgcggcttg
     3361 ccgccgacgg ggccgacttc gccgtcgggt gcggctacaa gtttttgaac ggcggacccg
     3421 gggcgccggc gttcctgtac atcgcgcagc gctggcatgg agaggtgcgc acgccgatct
     3481 ccggctggtt cgggcatgcg cggccgttcg aattcctgcc gggctacgaa gccgcgcccg
     3541 gcatcgcgcg cgcgctggcc ggcacgccgc cgatcatcag catggcggcg ctggaagcgg
     3601 gcgtggaact cgcgctcgag gtatcgatcg agcagctgcg cgccaagagc atgcgccagg
     3661 ttgaactgct ggctgcgctg gcggcgcaag agctggctga attcggtttc accgacatca
     3721 cgccgcgcga ggcgcagcag cgcggcagcc agttgagcct gatgcacgcg caggcctggc
     3781 cgatcacgca ggcgctggcc gcgcgcaacg tgattggcga tttccgcacg cccgacgtgc
     3841 tgcgcctggg cgtgacgccg atgtacctgg gttatgccga actgtgggac gcgatctgcg
     3901 cacttcggca ggtgatggtc gcggaagaat ggcgccagcc gcagcaccag cagcgcacgc
     3961 gggtgacctg agatgggaga tgccacgcag gccgccgcca ccggctggtt ccgcaacctg
     4021 ccgatcgggc gcaagctggc cgccgtggtc atcgcctacg tatcgatcat cgcggtgctg
     4081 ctgctggtgg tgcgcgcgtc tctcgacatc accagcggcg tgcgtgccta tgtcggcggc
     4141 gaaggcttgt ggtcgaaagg gcagaaggac gcggtgtatt acctcacccg ctacgcgcgc
     4201 agccacgatc agcacgacta cctgaaatac cgtgaagccc tcgccgttcc gctgggcgac
     4261 ggcgtggcgc ggcgtgaact gctcaagcct gccttcgacc acgacaccgc ggcgcgcggc
     4321 ttcatcgagg gcgccaacga cgccgccgac gtcgattaca tgatcgacct gttccggcgc
     4381 ttccgccggg tcagctacat ggcgcaggcc atcgacatct ggacccgcgc cgaggcgggc
     4441 atcatcgaga tgcagacggc cggcgacgag ctgcatgggg cagtcgccgc cggcacgctg
     4501 accccggacg aggaaaccgc gatcatcggc cgcatcgaaa cgatcaaccg ccggctgacg
     4561 ccgctcgagc atgatttctc ggccaccctg ggcgaggcgg cccgctggat ccgccaggtg
     4621 ctgcaggcgg tcatcgtcct cgcaagcatc ttgttgctgg tggctggcct cgccgtatcg
     4681 ctgcgcgtga cgcgcatgct gcgcggcgac atcgagcagc tgcgccgcgg cgccgcgcgg
     4741 gtggccgccg gcgatctcag ccagccggtg ccgcccggtg cgcgcgacga gctcggcgag
     4801 ctggccgccg cattcaacga catgatcgag caccggcgtg gcgccgagga ggcgctgcgc
     4861 agcgccaacg acttccggca gaaggtgatg gaaagcgcga ccaatgccat ctacgcgctc
     4921 gacctgcagg gccgcttcac cctgatcaac cgccgcacct gtgaaatcac cgggcgcagc
     4981 gaggccgaac tgctgggcca tcatttcggc atgttgttt
//
LOCUS       DASZZC010000325         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1245816,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000325 DASZZC010000000
VERSION     DASZZC010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245816"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VHE37_06830"
     CDS             <1..793
                     /locus_tag="VHE37_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT35278.1"
                     /translation="AVKIGRGEIELRGVSFRYHDGAQVFREVNLRIAPGQRVGLVGYS
                     GSGKSTFVNLILRLFEPQQGAILIDGHDLRSITLDSLHSQIGLIPQDPGLFHRSVADN
                     IRYGRGGASQAEVEDAARRAEAHGFISALPQGYDAMVGERGVKLSGGQRQRIAIARVL
                     LKNAPVLILDEATSSLDSVTEQAIPRALDQAMAGKTVIVVAHRLSTVAHLDRILVFDG
                     GRVVEDGSHAQLLAQRGHYYTLWSRQADGFLPESEDAASSETEPA"
     gene            complement(806..>1744)
                     /locus_tag="VHE37_06835"
     CDS             complement(806..>1744)
                     /locus_tag="VHE37_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35279.1"
                     /translation="ARLYKVDLSGDTITRGAVTVVTKQTLSSDKFLEVAGQGLNPKAA
                     ATWQITPDVLTYASASRGFQYGGVNLQAATLSLSAADHPTFKSSDLWNYEVGARTDWL
                     DRTLRLDLTLFHLDWNKPQVFQVDDSRTGLNGVYIDNVGAARGNGVEGTFRYLTPIPG
                     LSLSIAGSYIVLKTATDFITAAGGTVPSGTDMPLSPRVQTSTTLNYTNYFGPWVVAAA
                     AAQQYQGKAYNNIAHTFEVYDYSIYNLSLNLSRPDLPLAPSLMLIGSNLTDVRGITGI
                     NGIPRDLASGGVPTSSFPPTTYTRPRSISLRFTAEF"
BASE COUNT          277 a          563 c          623 g          281 t
ORIGIN      
        1 ggcggtgaag atcgggcgcg gcgagatcga gctgcgcggc gtgtcgttcc gttaccacga
       61 cggcgcacag gtgttccgcg aggtgaacct gcgcatcgcg cccggccagc gcgtcgggct
      121 ggtcggctac tcgggctcgg gcaaatccac cttcgtcaat ctcatcctgc ggctgttcga
      181 gccgcagcag ggcgcaatcc tgatcgacgg ccacgacctg cgctcgatca cgctcgactc
      241 gctgcactcg cagatcgggc tgatcccgca ggaccccggg ctgtttcacc gcagcgtggc
      301 cgacaacatc cgctacggtc gcggcggcgc cagccaggcc gaggtggaag atgccgcgcg
      361 ccgcgccgag gcgcatggct tcatcagcgc gctgccgcag ggctacgacg ccatggtcgg
      421 cgagcgcggc gtgaaactct ccggcgggca gcgccagcgc atcgccattg cgcgcgtgct
      481 gctgaagaac gcgccggtgc tgatcctcga cgaagccact tccagcctgg actcggtgac
      541 cgagcaggca atcccgcgcg cgctggacca ggccatggcc ggcaagacgg tgatcgtggt
      601 ggcgcaccgg ctgtccacgg tggcgcacct ggaccgcatc ctggtgttcg acggcggccg
      661 cgtcgtcgag gatggcagcc acgcgcagct gctggcgcag cgcgggcact actacacgct
      721 gtggagccgg caggccgatg gcttcctgcc ggagagcgag gacgcggcat catccgagac
      781 ggaaccggcc tgaccggccg tcggctcaaa actccgcggt gaagcgcagg ctgatcgagc
      841 gcggacgcgt gtaagtcgtc ggcggaaacg agctggtcgg cacgccaccg ctggcaagat
      901 cgcgcgggat gccattgatg ccggtgatgc cgcgcacgtc ggtcaggttc gagccgatca
      961 gcatcagcga gggcgccagc ggcagatcgg gccgcgacag gttcaggctc aggttgtaga
     1021 tggagtagtc gtacacctcg aaggtgtgcg caatgttgtt gtacgccttg ccctggtact
     1081 gctgcgcggc ggcggcggcg acgacccagg gtccgaaata attcgtgtag ttcagcgtgg
     1141 tcgaagtctg cacgcgcggc gacagcggca tgtcggttcc cgagggcacc gtgccgccgg
     1201 cggcggtgat gaagtcggtc gccgtcttca gcacgatgta cgagccggcg atgctcaggc
     1261 tcaggcccgg gatcggcgtc aggtagcgga aggtgccttc cacgccgttg cccctggcgg
     1321 cgccaacatt gtcgatataa acgccgttga gccccgtgcg gctgtcgtcc acctggaaca
     1381 cctgcggctt gttccagtcc aggtgaaaca gcgtcaggtc cagccgcagc gtgcggtcga
     1441 gccagtcggt cctggcgccc acctcgtagt tccacaggtc gctggacttg aacgtcgggt
     1501 ggtcggccgc ggacagcgag agggtggcgg cctgcaggtt cacgccgccg tactggaacc
     1561 cgcgcgaggc gctcgcatag gtcagcacat ccggcgtgat ctgccaggtg gccgcggcct
     1621 tgggattgag cccctggccg gccacttcga ggaatttgtc cgacgacagg gtctgcttgg
     1681 tgacgacggt taccgcgccg cgcgtgatgg tgtcgccgga caggtccacc ttgtacaggc
     1741 gggc
//
LOCUS       DASZZC010000326         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_730304,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000326 DASZZC010000000
VERSION     DASZZC010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730304"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VHE37_06840"
     CDS             <1..338
                     /locus_tag="VHE37_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT35280.1"
                     /translation="EAATLFSLKKMGCRLTLSQFGAGKGSLETLQSLPLDFIKLDVKL
                     TEEASKSDEARGKLKQVVGLAAPRNIGTISTRVADAMTLAELWKLGVNFVQGHYVHEP
                     EIVVEEKRR"
     gene            complement(352..1959)
                     /locus_tag="VHE37_06845"
     CDS             complement(352..1959)
                     /locus_tag="VHE37_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVT35281.1"
                     /translation="MKRIESSLNTRSSEFQANAAHNRKLADEFKAKQEAVRHTRPQRD
                     IDRLRQQNKMLVRERLDQLLDPGTPFLELSSLAANEAYDGEVPGAGCVTGIGIVSGRE
                     VLVNAGDASVKGGAWYPLTIKKTVRAMDIAIENRLPVVHLCDSAGGFLPLQADLFADK
                     YYAGRIFRNQCILSKMGVQQVAVVLGHCTAGGAYVPGLSDYNVIVRGTGAIFLAGPPL
                     VKAATGEENTVDELGGCDMHTSVSGTADYPAGSEEEAIAIARDIVGQFKRPQKTHIEF
                     ETPEAPYYDPAELYGVLPRDIKQQFDMREVIARLVDGSRFHEYQPNYGKTLICGYAHL
                     WGYKIGILANNGVLFNDSALKGTHFIELCNQNKTPILFLQNITGFMVGREYERRGITK
                     DGAKMIMAVSCSEVPKFTVMCHGSFGAGNYGMSGRAFDSRFLFSWPQSQISVMGAEQA
                     ANTLADVKIRQLAKQGKKLSQADVDAIRDPVLGEFKRKQSAYWSTSEIWDDGILDPVD
                     TRNALGIALSASLNAPIADPRYGIFRM"
     gene            complement(1956..2507)
                     /locus_tag="VHE37_06850"
     CDS             complement(1956..2507)
                     /locus_tag="VHE37_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVT35282.1"
                     /translation="MHHAFKLGDAEHNVELSRAARGYRLHIGERVVPVALANDELVIG
                     GQRKRVVIATRGDDVFVHVEGEAFQLHYEHPLERLAHQGAGAADDVIRAPMPGSVVSV
                     AVKPGDTVTRGQTVLVMESMKMETTLTAPRDGVIEAVGYAVAQTFDRDAVLIKLEAVG
                     AGLARDPEPQSRARRAPTKESGT"
     gene            complement(2508..4001)
                     /locus_tag="VHE37_06855"
     CDS             complement(2508..4001)
                     /locus_tag="VHE37_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVT35283.1"
                     /translation="MTDVKSWPFDTVLVANRGEIAVRVLRTVQSLGLKGAVVYHAADR
                     GSTAVAMADAAVEITGATPVAAYLDAQQILAAAKSVGAGAIHPGYGFLSENSKFARAV
                     EAAGIVFVGPRPEQIELMGDKVRARNFVQKAGFPVAPSAIEDDDPKTFNERARKVGAP
                     LLIKPSAGGGGKGMRIVRDLKNLDAEIERGRSEGQRYFGDGRLYVEKYIENPRHIEVQ
                     VMGDAHGNVVHFFERECSVQRRFQKIVEETPSPALTPELRRSICETAAGIARSAGYRN
                     AGTVEFIFGAGGEYYFLEMNTRLQVEHPVTEQITGTDLVLQQLRVAAGEKLGYAQDDI
                     KSSGHSIEFRIYAEDPGRGYTPTTGPVLKLQPPAGEGVRVDSGIVEGGEVTAAFDPML
                     AKLIITAPDRSAAIARADAALRDYVLLGCKTNTAFLRRLIAHPAFVAGEIHTGFLDAH
                     PEIAADPALAQPTLDALLAAAALSTRPMRDVADAVPDMHAAIGGWRN"
     gene            complement(3998..>4451)
                     /locus_tag="VHE37_06860"
     CDS             complement(3998..>4451)
                     /locus_tag="VHE37_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HVT35284.1"
                     /translation="LLKVLENWYDEMFGDYAREVAGARGARARLRLLVWRHLRTVKVH
                     GALCRLVFLEFRTGRDYRDSPVHAANRRYTQQLLNVLEEGVTSGEFRDDIPLTLLRDM
                     IYGGIEHHTWNYLCGRGGIDIERIADEMVEVLCDGIKPRAAKKSAGKK"
BASE COUNT          688 a         1495 c         1458 g          810 t
ORIGIN      
        1 aggaagcggc cacgctgttc tcgctgaaga agatgggctg ccggctgacg ctgtcgcagt
       61 tcggcgccgg caagggctca ctggaaacgc tgcagagcct gccgctggat ttcatcaagc
      121 tcgacgtcaa gctgaccgag gaggcgagca agtccgacga ggcgcgcggc aagctcaagc
      181 aggtggtggg cctggccgcg ccgcgcaaca tcggcaccat ctccacccgc gtggccgacg
      241 ccatgacgct ggccgagctg tggaagctgg gcgtcaactt cgtgcagggc cactacgtgc
      301 acgagccgga gatcgtggtc gaggagaaga ggcgctgagc gccgctgcac actacatgcg
      361 gaagatgccg taacgcggat cggcgatcgg tgcattgagc gacgcgctca gggcgatgcc
      421 cagcgcattg cgcgtgtcca ccgggtcgag gatgccgtcg tcccagattt ccgatgtgct
      481 ccagtaggcg ctctgcttgc gcttgaactc gcccagcacc gggtcgcgga tggcgtcgac
      541 atcggcctgc gacagcttct tgccctgctt ggccagctgg cggatcttca cgtcggccag
      601 cgtgttggcg gcctgctcgg cgcccatcac ggaaatctgc gactgcggcc aggagaagag
      661 aaaccgcgaa tcgaacgcgc gcccgctcat gccgtagttg ccggcgccga agctgccgtg
      721 gcacatcacg gtgaacttgg gcacttcgct gcacgacacc gccatgatca tcttggcgcc
      781 gtccttggtg atgccgcggc gctcgtactc gcgcccgacc atgaagccgg tgatgttctg
      841 caggaacagg atgggcgtct tgttctggtt gcacagctcg atgaagtgag tgcccttgag
      901 cgcgctgtcg ttgaacagca cgccgttgtt ggcgaggatg ccgatcttgt agccccacag
      961 gtgcgcatac ccgcaaatga gggtcttgcc gtaattgggc tgatattcat gaaatcttga
     1021 accatcgacc aggcgcgcga tcacctcgcg catgtcgaac tgctgcttga tgtcgcgcgg
     1081 cagcacgccg tacagctcgg cggggtcgta gtacggcgct tccggcgtct cgaattcgat
     1141 atgcgtcttc tgcggccgct tgaactggcc gacgatgtcg cgcgcgatgg cgatcgcttc
     1201 ctcttcgctg cccgccgggt aatccgccgt gcccgacacc gaggtgtgca tgtcgcagcc
     1261 gccgagttcg tcgacggtgt tctcctcgcc ggtggccgcc ttcaccagcg gcggcccggc
     1321 gaggaagatg gcgccggtgc cgcgcacgat cacgttgtag tccgacaggc ccggcacgta
     1381 ggcgccgccg gcggtgcaat ggcccagcac gaccgcaacc tgctgcacgc ccatcttcga
     1441 caggatgcat tgattgcgga agatgcggcc tgcgtaatac ttgtccgcga acaggtccgc
     1501 ctgcagcggc aggaagccgc cggcggaatc gcacaggtgc acgacgggca agcgattctc
     1561 gatagcgatg tccatcgccc gcacggtttt cttgatcgtc agcggatacc aggcgccgcc
     1621 tttcaccgac gcgtcgcctg cgttcaccag cacttcacgg ccggagacaa ttccgattcc
     1681 ggtgacgcag ccggcgcccg gcacttcgcc gtcgtaggct tcgttggcag cgagcgagga
     1741 cagctccagg aacggcgtgc ccggatcgag cagctggtcg aggcgctcgc gcaccagcat
     1801 cttgttctgc tgacgcagcc ggtcgatgtc gcgctgcggg cgcgtgtggc gcaccgcttc
     1861 ctgcttggcc ttgaactcgt ccgcaagttt cctgttgtgc gcggcgttgg cctggaactc
     1921 gctgctacgc gtgttcaggc tggattcgat gcgtttcatg tgccgctctc cttcgtagga
     1981 gcgcgccttg cgcgcgattg cggttccgga tcgcgggcaa ggcccgctcc tacagcctcc
     2041 agcttgatca gcaccgcgtc gcggtcaaag gtctgcgcca cggcgtagcc caccgcttcg
     2101 atcacgccgt cgcgcggcgc ggtcagcgtg gtttccatct tcatgctctc catcaccagc
     2161 accgtctggc cgcgtgtcac cgtgtcgccc ggtttgacgg cgacgctgac caccgagccc
     2221 ggcatgggcg cgcggatgac gtcgtcagct gcaccggcgc cctggtgcgc aaggcgctcc
     2281 agcggatgct cgtagtgcaa ttgaaaggct tcgccctcga cgtgcacgaa cacgtcgtcg
     2341 ccgcgcgtgg cgatgaccac gcgcttgcgc tggccgccga tgaccagttc gtcgttcgcc
     2401 agcgcaaccg gcacgacgcg ttcgccgatg tgcagccggt agccgcgcgc ggcgcgggac
     2461 agttcgacgt tgtgctcggc gtcgccgagc ttgaatgcat ggtgcattta atttctccac
     2521 ccgccgatgg ccgcgtgcat atccggcact gcgtctgcga catcgcgcat cggacgcgtc
     2581 gacagcgcgg ccgctgcgag cagcgcatcg agggtcggtt gcgccagggc cggatcggcg
     2641 gcgatctccg ggtgcgcgtc gagaaagccg gtatggattt cgccggcgac gaaagccggg
     2701 tgcgcgatca gcctgcgcaa aaacgccgtg ttggtcttgc agccgagcag cacgtaatcg
     2761 cgcagcgcgg catcggcgcg cgcgatggct gcgctgcggt ctggtgcggt aatgatgagc
     2821 ttggccagca tcggatcgaa cgcggcggtg acctcgccgc cttcgacgat gccgctgtcc
     2881 acgcgcacgc cctcgcccgc cggcggctgc agcttcagca caggccccgt cgtcggcgtg
     2941 tagccgcggc ccgggtcctc ggcgtagatg cggaattcga tcgagtggcc ggatgacttg
     3001 atgtcgtcct gcgcgtagcc cagcttttcg cccgcggcga cgcgcagctg ttgcagcacc
     3061 aggtcggtgc cggtgatctg ctcggtcacc ggatgttcca cctgcaggcg cgtgttcatc
     3121 tcgaggaagt agtactcgcc tccggcgccg aagatgaact ccaccgtgcc ggcgttgcgg
     3181 taacccgcgc tgcgcgcgat gccggcggcc gtctcgcaga tgctcctgcg cagttccggc
     3241 gtgagcgccg gcgacggcgt ttcctcgacg attttctgga agcggcgctg caccgagcat
     3301 tcgcgctcga agaaatgcac cacgttgccg tgcgcgtcgc ccatcacctg cacctcgata
     3361 tggcgcgggt tctcgatgta tttctccacg tacaggcggc catcgccgaa gtagcgctgg
     3421 ccctcgctgc ggccgcgctc gatctcggca tcgaggtttt tcaggtcacg cacgatgcgc
     3481 atgcccttgc cgccgccgcc ggctgaaggc ttgatcagca gcggcgcgcc gaccttgcgc
     3541 gcgcgctcgt tgaaggtctt gggatcgtcg tcctcgatcg ccgacggcgc gaccgggaag
     3601 ccggccttct gcacgaagtt gcgcgcgcgc accttgtcgc ccatcagctc gatctgctcc
     3661 ggcctggggc cgacgaagac aattcccgcc gcctcaacgg cgcgtgcgaa cttggaattc
     3721 tccgagagaa atccatagcc aggatggatc gcgccggcac ccaccgattt tgccgccgcc
     3781 aggatctgct gcgcatcgag atacgcggcc accggcgtcg cgccggtgat ctccaccgcg
     3841 gcgtcggcca tggccaccgc ggtcgagccg cggtcggcgg catggtagac cacggcgcct
     3901 ttcaggccca gcgactgcac cgtgcgcaac acgcgcaccg cgatctcgcc gcggttggcc
     3961 accagcaccg tatcgaacgg ccaggatttc acgtcggtca ttttttcccc gctgatttct
     4021 tcgccgcgcg cggcttaatg ccgtcgcaca gcacctccac catctcgtcg gcgatgcgct
     4081 cgatgtcgat gccgccgcgc ccgcacaggt aattccaggt gtgatgctcg atgccgccgt
     4141 agatcatgtc gcgcagcagc gtgagcggaa tgtcgtcacg gaactctccg ctggtgacgc
     4201 cttcttccag cacgttcagc agctgctgcg tgtagcggcg gttggccgcg tgcaccggcg
     4261 agtcgcggta gtcgcggccg gtgcggaatt ccaggaacac cagccggcac agcgctccgt
     4321 gcaccttcac cgtgcgcagg tggcgccaga ccagcaggcg caggcgggca cgtgcgccgc
     4381 gcgcgccggc gacctcgcgc gcgtagtcgc cgaacatctc gtcgtaccag ttctccagca
     4441 ccttcagcag c
//
LOCUS       DASZZC010000327         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_29448,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000327 DASZZC010000000
VERSION     DASZZC010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29448"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="VHE37_06865"
     CDS             complement(<1..1939)
                     /locus_tag="VHE37_06865"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVT35285.1"
                     /translation="MRHFFSAAILCAASAAHAATPASGTLTEANTEDYPLTYAAGPFS
                     DDNLTATIGEPALIRCSGGNNPCDQYALTFDLPADYADKHPDTYVRIRFKRDVPGDSL
                     LHYVVTDAGTAQTGQNYFAYGNDIADDTQEIYLLPFPGQRTLTVESVPSAVTSSNETV
                     SIALVHIAGEPRHPELPYAAHGNGPRFATYRPDKSPGLGLAAAEPSIGFNPKTGSVFY
                     INTLDTLRVKFDDSTVPARDTWASKHGNFSDIATLDPILYVDQTTGRVFSLQLAGTQS
                     LSEYSDDDGETWLPGGNGFPSSGVDHQSLGAGPYPATTTIPVIHPLYPNAVYYCSQGV
                     AASFCSRSDDGGVTFNPFVPIYTATDCVGLHGHVKVGPTGTAYVPNKGCGLGTESVLG
                     PGVPGLIVSEDAGLTWKVKLVSMAPSDLSTKSDPSVAIGKDDTVYYAYAPLDDHLRVA
                     VSKDHGDTWINDLDVGAIAGIHNAEFPAMVAGDAGRAAVAFLGDTFPVDSADDSGMDF
                     PGVWHFYIASTFDYGAHWFVEQVGDDLSQGPGGIASGGANRNLLDFIDATIDDQGRVL
                     VSYADGCMGGCSATHQGHFTRVAVIARQTGGPRMYAAFDPVEPALPAAPRVTGYRTQD
                     YTVLDIAADSGGAPISGYTIIR"
     gene            2039..>2767
                     /locus_tag="VHE37_06870"
     CDS             2039..>2767
                     /locus_tag="VHE37_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVT35286.1"
                     /translation="MTLTELRYLVNLDRERHFGRAAERSFVSQPTLSVALKKLEEELG
                     VALFERNRGEVKPTAVGVRVIEQARRVLAESQRLEELARAGKDELAGPLRLGAIYTVA
                     PYLLPGLVPVLRDNAPQMPLVIEENFTAKLLEQLRDNELDVIVIALPVDLAGLSAWPL
                     YDEDFVVVLPAEHRWAKEKQIVAQRLAEEKLLLLGPGHCFREQVVAACPKCIDENSSG
                     PHPLTGSSLETIRHMVVSGLGITVL"
BASE COUNT          438 a          874 c         1002 g          453 t
ORIGIN      
        1 cgcggatgat ggtatagccg cttatcggtg cgccgccgct gtcggcggcg atgtcgagca
       61 cggtgtagtc ctgcgtgcgg tagccggtca cgcgcggcgc cgccggcagc gccggttcca
      121 ccgggtcgaa cgccgcgtac atgcgcgggc cgccggtctg gcgcgcaatc accgccacgc
      181 gcgtgaagtg gccctggtgc gtggccgagc agccgcccat gcagccgtcg gcatagctca
      241 ccagcacgcg gccctggtcg tcgatggtgg cgtcgatgaa gtcgagcagg ttgcggttgg
      301 cgccaccgct ggcgatgccg cccggaccct gtgagagatc gtcgcccacc tgttcgacga
      361 accagtgcgc gccgtaatcg aaggtgctgg cgatgtagaa atgccacacg ccgggaaagt
      421 ccatgccgga atcgtcggcg ctatcgaccg ggaaagtgtc gccgaggaag gccaccgcgg
      481 cgcgcccggc atcgcccgcc accatcgccg ggaactcggc gttgtggatg ccggcgatgg
      541 cgccgacgtc gaggtcgttg atccaggtgt cgccgtggtc cttcgacacc gccacgcgca
      601 ggtgatcgtc cagcggcgcg taggcgtagt acacggtgtc atccttgccg atggccaccg
      661 acggatcgct cttggtgctc aggtccgacg gtgccatcga caccagcttg accttccagg
      721 tcaggccggc gtcttccgaa acgatcagcc ccggcacgcc gggacccagc accgactcgg
      781 tacccaggcc gcagcccttg ttcggtacat aggcggtgcc ggtcggcccg accttgacgt
      841 ggccgtgcag gcctacgcaa tcggtggcgg tgtagatcgg cacgaacggg ttgaaggtga
      901 cgccgccatc gtcgctgcgc gagcagaacg acgctgcgac gccctgcgag cagtagtaca
      961 ccgcgttggg atacagcgga tggatcaccg ggatggtcgt cgtcgccggg tacggcccgg
     1021 cgccgagcga ctggtgatcg acgccgctgg agggaaagcc gttgccgccg ggcagccagg
     1081 tctcgccatc gtcatcggag tattcggaca agctctgcgt gccggcgagc tgcagcgaga
     1141 acacgcggcc ggtggtctgg tcgacgtaca ggatcgggtc gagcgtggcg atgtcgctga
     1201 agttgccgtg cttgctggcc caggtgtcgc gtgccggcac ggtgctgtcg tcgaacttca
     1261 cgcgcagcgt gtcgagcgtg ttgatgtaga acacgctgcc ggtcttcggg ttgaagccga
     1321 tcgaaggctc cgccgcggcc aggcccaggc ccggcgactt gtccgggcga taggtggcga
     1381 agcgcggccc gttgccatgc gccgcgtagg gcagttccgg gtgccgcggt tcgccggcga
     1441 tatgcaccag cgcgatgctc accgtctcgt tgctgctggt gaccgctgat ggcaccgact
     1501 cgacggtcag tgtgcgctgg ccggggaacg gcagcaggta gatctcctgc gtgtcgtcgg
     1561 caatgtcgtt gccgtaggcg aagtagttct gcccggtctg ggccgtgccg gcgtcggtga
     1621 ccacgtagtg cagcaggctg tcgcccggca cgtcgcgctt gaagcggatg cgcacgtagg
     1681 tgtcgggatg cttgtccgcg taatcggccg gcaggtcgaa ggtcagcgcg tactggtcgc
     1741 acgggttgtt gccgccgctg cagcggatca aggccggctc gccgatggtg gcggtgaggt
     1801 tgtcgtcgct gaacggaccg gccgcgtagg tcagcggata gtcctcggtg ttggcctcgg
     1861 tgagcgtgcc cgacgccggc gtggcggcat gggcagccga tgccgcgcac aggatggcgg
     1921 ctgaaaaaaa gtgacgcatg ctaacctccc ctggacccgc accgatgcag cgcgcccgat
     1981 gcggcaaggg cgcatcttag cgccggcccg tctcgccgcc ggaaggtaaa gaacactcat
     2041 gacgctgacc gagttgcgct acctggtgaa cctggaccgc gaacggcatt tcggccgcgc
     2101 cgcagagcgc tcgttcgtgt cgcagccgac gctgtcggtg gcgctgaaaa aactcgagga
     2161 agaactgggc gtggcactgt tcgagcgcaa ccgcggcgag gtaaaaccca ccgccgtcgg
     2221 tgtacgcgtg atcgagcagg cgcgccgcgt gctggccgag tcgcagcgcc tggaagaact
     2281 ggcgcgcgcc ggcaaggacg agctggccgg tccgctgcgc ctcggcgcga tctacacggt
     2341 ggcgccgtac ctgctgcccg ggctggtgcc ggtgctgcgc gacaacgcgc cgcagatgcc
     2401 gctggtgatc gaggaaaatt tcacggccaa gctgctggaa cagctgcgcg acaacgagct
     2461 ggacgtgatc gtcatcgcgc tgccggtgga tttggcgggc ctgtcggcct ggccgctata
     2521 cgacgaggat ttcgtggtgg tgctgccagc cgagcatcgc tgggcgaagg aaaagcagat
     2581 cgtcgcgcag cgcctggcgg aggaaaaact gctgctgctc ggccccggcc actgcttccg
     2641 cgagcaggtg gtggcggcct gccccaagtg catcgacgag aacagcagcg ggccgcatcc
     2701 gctcaccggc agcagcctgg aaaccatccg ccacatggtg gtgagcgggt tgggcatcac
     2761 cgtgctg
//
LOCUS       DASZZC010000328         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_219609,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000328 DASZZC010000000
VERSION     DASZZC010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219609"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VHE37_06875"
     CDS             <1..710
                     /locus_tag="VHE37_06875"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVT35287.1"
                     /translation="RADLGGFGPAVGTVGKNDVAAHGLGDDPWMEKRNRPPRFPAPFS
                     CNTLGMQTPLTIHDEPLPYANRLQARALADIDLVVIHCTELPDLATAREYGERIVHAS
                     GTGNSGHYYVDRDGSVLRYVPDERVAHHVRGHNEHAVGIELVNNGRYPDWLDSRHQTM
                     SEPYPAAQMDALLALLAHLRGALPNLRAIAGHEDLDTDTVAASNDASKQVRRKLDPGP
                     MFPWPRVLDACGLQRLR"
     gene            739..1575
                     /locus_tag="VHE37_06880"
     CDS             739..1575
                     /locus_tag="VHE37_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVT35288.1"
                     /translation="MPEFVVQPLLQSTLVGAHDVHCRGECRHRSTEECSAATHLVFPY
                     RGLFMRHVGSTQSVADANHVLFFNGGEGYQVSHPATGGDACLSLSISQPLLQELAPAA
                     LLQRRDTPMFRNQSLRIDERAQVLVALLRHSLRSGAAEPLEAESLALTLVCRSLGPRT
                     THAAGSTHARQRLVDRAKLLLASDPTRRWTLAEIASEVGGSPVYLTQAFKQVEGMPLY
                     RYHLRLRLARALDLIADCEDVAALALDLGFSSHSHFSAAFRQAYGRTPSEFREAALAK
                     SA"
     gene            1659..1802
                     /locus_tag="VHE37_06885"
     CDS             1659..1802
                     /locus_tag="VHE37_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35289.1"
                     /translation="METKEATCAVCDYPLDGNAIKVTIGGRTVEVCCEECAQKLRDAE
                     AQA"
     gene            1879..>2147
                     /locus_tag="VHE37_06890"
     CDS             1879..>2147
                     /locus_tag="VHE37_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HVT35290.1"
                     /translation="MTAHPSCRIAVYGAYGHTGRFVLNELRRRGFVPIACGRDAGKLD
                     ALAESIDTETRVAAVDDPAALDAAFAGTAAVLHCAGPFLDTAVPVL"
BASE COUNT          365 a          748 c          671 g          363 t
ORIGIN      
        1 agcgcgctga ccttggtggc ttcgggcccg ctgtcggaac cgtgggaaag aatgacgtgg
       61 ccgcgcatgg actgggcgat gatccgtgga tggaaaagcg taacaggccg ccccgctttc
      121 ccgcgccgtt ttcatgcaac actcttggca tgcagacgcc actgacgatc cacgatgaac
      181 cgctgcccta tgccaaccgg ctgcaggcgc gcgcgctggc cgatatcgat ctcgtggtca
      241 tccattgcac ggagttgccc gacctcgcga ccgcgcgcga atacggcgaa cgcatcgtgc
      301 acgcgagcgg caccggcaac agcgggcact attacgtcga tcgcgacggc agcgtgctcc
      361 gctacgtgcc cgacgaacgt gtcgcgcacc acgtgcgcgg tcacaacgaa catgccgtcg
      421 gcatcgagct ggtcaacaac ggccgctatc ccgactggct ggattcgcgc catcaaacga
      481 tgagcgagcc gtatccggcg gcgcagatgg acgcgttgct ggcgttgcta gcgcacctgc
      541 gcggcgcatt gccgaatctt cgcgccatcg ccggccacga agatctcgac acggacacgg
      601 ttgccgccag caacgatgct tcgaagcagg tacggcgcaa gctcgacccc ggcccaatgt
      661 tcccatggcc gcgcgtgctc gatgcctgcg gcctgcaacg gctgcgctag actgcatcga
      721 cgccagtcca tcctcgtcat gccggagttc gtcgtccagc cgctgctgca atcgaccctc
      781 gtcggggcgc acgacgtgca ttgccgcggc gagtgccggc atcgcagcac cgaggaatgt
      841 tcggcagcca cgcacctggt gttcccctac cgcggcctgt tcatgcgtca cgtcggcagc
      901 acgcagtcgg tggccgatgc caaccacgtg ctgttcttca acggcggcga gggttaccag
      961 gtcagccatc cggcgacggg cggcgacgcc tgtttgtcgc tttcgatttc gcaaccgctg
     1021 ctgcaggaac ttgcgccggc cgcgttgctg cagcgtcgcg acacgccgat gttccgcaac
     1081 caatcattgc gcatcgacga acgtgcgcaa gtgttggtgg cgctacttcg ccacagcctg
     1141 cgcagcggcg ccgccgaacc gttggaagcc gaaagcctgg cattgacgct cgtgtgccgc
     1201 agccttggcc cgcgcaccac ccatgcggcg ggttcgaccc acgcgcgcca gcggctggtc
     1261 gatcgtgcca agttgctgct ggccagtgac cccacccggc gctggacact ggccgaaatc
     1321 gcatcggaag tcggcggctc gccggtctat ctcacgcagg ctttcaagca ggtcgaaggc
     1381 atgccgctgt accgctacca ccttcggctg cgactggcgc gcgcgctgga cctgatcgcc
     1441 gattgcgaag acgtggccgc gctcgcgctg gacctcggct tctccagcca cagccatttc
     1501 agcgcagcat tccgccaagc ctacggccgc acgcccagcg agtttcgcga ggccgcgctg
     1561 gccaaatcgg cttgaatcgc taaatttcct gacagccgct ggaatcgccc ggtggttcca
     1621 tgtgcgtgcc cgattttggg catcaccgga gaacccccat ggaaacgaaa gaagccacct
     1681 gtgccgtctg cgactacccg ctcgacggca acgccatcaa ggtcacgatc ggcggccgca
     1741 ccgtcgaagt ctgctgcgag gaatgtgcac agaaactgcg cgacgcggaa gcgcaggcgt
     1801 gagcggcgct gcaccgtcat cggttgcaca cgggagcgcg ccgggcttgg tccggcgcga
     1861 ctcctggagc gaaatcgcat gaccgcacat ccatcctgcc gcattgcggt ctatggcgcc
     1921 tacggccata ccggacgctt cgtgctgaac gaacttcgcc gccgcggttt cgttccgatt
     1981 gcctgcggcc gtgatgcggg gaaactggac gcactggccg agtccatcga caccgaaacc
     2041 cgcgtggccg ccgtcgatga tcccgcagcg ctggacgccg ccttcgccgg caccgctgcc
     2101 gtcctgcact gcgcgggccc gttcctcgac accgcggtgc cggtgct
//
LOCUS       DASZZC010000329         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1121745,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000329 DASZZC010000000
VERSION     DASZZC010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121745"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VHE37_06895"
     CDS             complement(<1..1521)
                     /locus_tag="VHE37_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT35291.1"
                     /translation="MNCPSCHAENPGTARFCNQCGARLDQAPVPVPASVRQDYTPRHL
                     ADRILKNRGAMQGEKKRVTVLFCDIKGSTRLAQEAGAELWHAILDRFFSILSAAVHRY
                     EGTVNQYTGDGIMALFGAPIAHEDHAQRACFAALQMQAEVRRFADELRLQKGLNLSMR
                     VGLNTGEVIVGRIGDDLRMDYTAQGVTVNLAARMEHICEPGRIYLSRYTAALVEGYFR
                     LRDLGRMSVAGVDDPVEVYELEGEGSLRTRLDRSLARGGSQFVGRDRELAALLAALDR
                     VKAGDGQVVAVVGNAGIGKSRLCHEFTRACEQAGVAVHRATGVPYANALPLFPLQTLL
                     RSRIGISERTPPEDVRRMIAGTFLLQDAAHAAILPAVFDFLGVASSGERAPDQAGAVR
                     QKLVQLYAEYVPCVEHAQILLVEDLHFLDAASEEFMAALCARVHGTKTLLLVNYRPDY
                     VSEWLIPHLDEQVSVSALSHAQLEQLASSLLGGHASLEGLAVKIRERASGNPFFVEE"
     gene            1724..2719
                     /locus_tag="VHE37_06900"
     CDS             1724..2719
                     /locus_tag="VHE37_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HVT35292.1"
                     /translation="MLLLVGAGAVGTILAGHLAQAGRETLKLYTRDKDLASFNSLREL
                     HIDYAQRGHAPLALPRPELATSLELCCVEYLLICVKFPQLAALLKQLPAIPAGCTVVS
                     TLNGAASLRLMRERWPQARVIPLTVMYNGQLLGPLHAQLTTRAEVIAGGDDCRLARLF
                     RGSGMHARHADGDSAVWGKLLINLANALCALTHTTFKDLLTDPDLRMLYVEVLDEAVA
                     VLNASGHDFQLPIAVPYRAYRQLLLRGGKIPWWFARAKNGLEEGAYPSMVSDVEHGRV
                     TEVEQLNGEIVQLAAAYGQRAPVNQRIVAAVKQLQTNHPPQYLTPKQLRHELLRP"
     gene            complement(2767..3033)
                     /locus_tag="VHE37_06905"
     CDS             complement(2767..3033)
                     /locus_tag="VHE37_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HVT35293.1"
                     /translation="MTQQQSGSSNRGFAGMDARKQREIAAAGGRAAHQQGTAHEFSSE
                     EAREAGRKGGQAAHASGHAHEFSSEEAREAGRKGGQASGGVNRR"
     gene            complement(3290..3502)
                     /locus_tag="VHE37_06910"
     CDS             complement(3290..3502)
                     /locus_tag="VHE37_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35294.1"
                     /translation="MPPGNSPAASSRLHRGGCTQKNFELSACAATQSAQLFRQGRIAM
                     SIRRPMRKREVLLIALCAVIAVSLGY"
     gene            complement(3513..4007)
                     /locus_tag="VHE37_06915"
     CDS             complement(3513..4007)
                     /locus_tag="VHE37_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT35295.1"
                     /translation="MKQLSTCFLLATCSMLGACSNQPDKFYSTFFLPKNPAPQKVQVA
                     DTPDCTVYFDLDSAKIRADQTATLDRCAQYLSKHPEQALALEGHADERGGNQHNLKLG
                     EQRAEAVKQALFQRGISGEHISVASLGEEHVAQPGEPAWSHSRRVDAQLAPVAPAIQP
                     GKGN"
     gene            4187..>4437
                     /locus_tag="VHE37_06920"
     CDS             4187..>4437
                     /locus_tag="VHE37_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35296.1"
                     /translation="MAAEQPDAGTQKRKPALSAEAIVAVGLLSVCAIVGGAWWLLQLR
                     AESAPADTAGSGAADAATDQVEVTASSGGSSMHWQSALQG"
BASE COUNT          720 a         1490 c         1497 g          730 t
ORIGIN      
        1 ctcctcgacg aagaacggat tgccgctggc gcgctcgcgg atcttcaccg ccaggccttc
       61 caggctggcg tggccgccga gcaggctcga cgccagctgc tcgagctgcg cgtgcgacag
      121 cgctgacacc gacacctgtt cgtcgaggtg cgggatcagc cattcgctga cgtagtcggg
      181 ccggtaattc accagcagca aggttttggt gccatgcacg cgcgcgcaca gcgcggccat
      241 gaattcctcg ctggcggcat cgaggaaatg caggtcttcc accagcagga tctgcgcatg
      301 ctcgacacag ggcacgtact cggcgtacag ctgcaccagc ttctgtcgta cggcgccggc
      361 ctggtcgggc gcgcgctccc cgctgctggc cacgccgagg aaatcgaaca ccgcgggcag
      421 gatggcggca tgcgcggcgt cctgcagcag gaaggtgccg gcgatcatgc ggcgcacgtc
      481 ttccggcggc gtgcgctcgg aaatgccgat gcgcgaacgc aacagcgtct gcagcgggaa
      541 cagcggcagc gcattggcgt acggcacgcc ggtggcgcgg tgcacggcga cgccggcctg
      601 ctcgcaggcg cgggtgaact cgtggcacag gcgcgacttg ccgatgccgg cgttgccgac
      661 cacggcgacg acctggccgt cgccggcttt cacgcggtcg agcgcggcca gcagcgcggc
      721 gagttcgcgg tcgcggccga cgaactgcga gccgccgcgc gccaatgaac ggtccagccg
      781 cgtgcgcagg ctgccctcgc cttccagttc gtacacttcc accgggtcat cgacgccggc
      841 cacgctcatg cggcccaggt cgcgcaggcg gaaataacct tcgaccagcg cggcggtgta
      901 gcgcgaaaga tagatgcggc ccggctcgca gatatgttcc atgcgcgcgg ccaggttcac
      961 cgtcaccccc tgcgcggtgt agtccatgcg caggtcgtcg ccgatgcggc cgacgatgac
     1021 ttcgccggtg ttgagcccca cacgcatgga gagattgaga cctttttgca ggcgcagttc
     1081 gtcggcgaag cggcgcactt ccgcctgcat ctgcagcgcg gcaaagcagg cgcgctgcgc
     1141 gtggtcctcg tgcgcgatcg gcgcgccgaa cagcgccatg atgccgtcac cggtgtactg
     1201 gttcaccgtg ccttcgtagc ggtgcaccgc ggcggacagg atcgagaaga agcggtcgag
     1261 gatggcgtgc cacagctcgg cgccggcttc ctgcgcgagt cttgtgctgc ccttgatgtc
     1321 gcagaacagc acggtgacgc gcttcttctc gccctgcatg gcgccgcggt tcttcaggat
     1381 gcggtcggcc aggtggcgcg gcgtgtaatc ctggcgcacg ctggccggca ccggcaccgg
     1441 cgcctggtcc agccgcgcgc cgcactggtt gcagaagcgc gccgttcccg ggttctcggc
     1501 atggcaggac gggcagttca tcgcgcggga gcatccattg gtcaacccgc gcatgataaa
     1561 gccagcgcaa ggcgcaaaat gccctggacc gcacccacct ttcgcatgtt ataatttatt
     1621 gtttgttttt aaatacagac aataatttat aacatcaaaa ataccggtgc ggtgaaagct
     1681 tttttcgccc gctgggcatc gcgggcggca caaggaggag cacatgctgc tactggtcgg
     1741 agccggcgcg gtcggcacca tactcgccgg ccacctggcg caggccgggc gcgagacgct
     1801 caagctgtac acgcgcgaca aggatctcgc gtcgttcaac tcgctgcgcg aactgcacat
     1861 cgactacgcg caacgcggac acgcgccgct ggcgctgccg cggccggagc tggccacctc
     1921 gctggaactc tgctgcgtcg aatacctgct gatctgcgtg aagttcccgc agctcgccgc
     1981 actgctcaag cagcttccgg caatacccgc gggctgcacc gtggtgtcga cgctcaacgg
     2041 cgccgcctcg ctgcgcctga tgcgcgagcg ctggccgcag gcgcgcgtga tcccgctcac
     2101 cgtgatgtac aacggccagc tgctcggccc gctgcacgcg cagctgacca cgcgcgcgga
     2161 agtgatcgcc ggcggcgacg actgccggct ggcgcgcctg ttccgcggca gcggcatgca
     2221 cgccaggcat gccgatggcg attccgcggt atggggcaag ctgctgatca acctggccaa
     2281 tgcgctgtgc gcgctgaccc acaccacctt caaggacctg ctcaccgatc ccgatctgcg
     2341 catgctgtac gtcgaggtgc tggacgaagc ggtcgccgtg ctcaacgcga gcggccatga
     2401 cttccagctg ccgatcgccg tgccgtaccg cgcctaccgc cagctgctgc tgcgcggcgg
     2461 aaaaatcccg tggtggttcg cgcgcgccaa gaacgggctg gaggaaggcg cgtatccgtc
     2521 gatggtgtcc gacgtcgagc acggccgcgt gactgaggtg gaacagctca acggcgagat
     2581 cgtgcagctg gcggcggcct acggccagcg ggcgccggtc aaccagcgca tcgtcgccgc
     2641 ggtgaaacag ctgcagacca accatccgcc gcagtacctc acgcccaagc agctgcgcca
     2701 cgaactgctg cgcccataaa aaaaggccgg ctgcagtcgg cagccggccg gcatcggcgc
     2761 agcgggttaa cgacgattga ctccgcccga cgcctggccg cccttgcggc cggcctcacg
     2821 cgcttcctcg gaggaaaact cgtgggcatg gccgctggca tgggcggcct ggccaccctt
     2881 gcggccggcc tcgcgcgcct cttcggaact gaactcatgg gcggtgccct gctggtgggc
     2941 tgcgcggccg ccggcggcgg caatctcgcg ctgcttgcgt gcgtccatgc ccgcaaagcc
     3001 gcggttgctg ctgcctgatt gctgctgggt cataaaacct cctgcgatga tcaaggtgga
     3061 acgaggcgat ggaaaaagac cgcccgatcc gcgcgaatcg cggtgtttca gtgatccatc
     3121 gtgatcctcg gaatatggga ctggaggctg ccgaacgagt tcacggatga gctccccgca
     3181 gggcagcggg cgcgcgcgca acccgccgga catcaacgca atttcgcgca gatttcgcgg
     3241 caccggccgc accaccgggc atgcgcggaa aaaatttcag cgcggcgttt tagtaaccca
     3301 gcgaaacggc gatgacggcg cacagcgcga tcagcagcac ctcgcgcttg cgcatcgggc
     3361 ggcggatgga catggcaatt ctcccctggc gaaaaagctg cgccgattgg gttgccgcac
     3421 acgcggaaag ttcgaaattt ttttgagtgc agccgccgcg atgaagccgg ctggatgccg
     3481 ccggggaatt tcccggcggc atccggcttg attcagttgc ccttgcccgg ctggatggct
     3541 ggcgccaccg gtgccagctg cgcatcgacc cggcggctgt ggctccacgc cggttcgccc
     3601 ggctgcgcca cgtgctcctc gcccaggctg gccacgctga tgtgctcgcc ggagatgccg
     3661 cgctggaaca aggcctgctt caccgcttcg gcgcgctgct cacccagttt caggttgtgc
     3721 tggttgccgc cgcgctcgtc ggcatggcct tccagcgcca gcgcctgctc ggggtgcttc
     3781 gacaggtact gcgcgcagcg gtccagcgtc gcggtctggt cggcgcggat cttcgcgctg
     3841 tcgaggtcga agtacaccgt gcaatcgggc gtgtcggcca cctgcacctt ctgcggcgcc
     3901 ggattcttcg gcaggaagaa ggtgctgtag aacttgtcgg gctggttgct gcatgcccca
     3961 agcatgctgc aggtggcgag caggaagcag gtgctgagtt gtttcatggt tgttctcggt
     4021 ctttggtgtg ggtgccgcgc cgcgatgata cgacggccat ggtcgcgcgg cgcattgccg
     4081 gccgcggcgc gccctcgcta tagtgctgcg caggggacgc gccacggcgc ggctattgca
     4141 tcgcaggaac ccggcaccag gaccgatccg cacactgctt ccgaccatgg ctgcagaaca
     4201 accagacgca ggcacgcaga aaaggaaacc cgcactcagc gccgaagcca tcgtggccgt
     4261 cggcctgctc agcgtgtgcg ccatcgtggg cggcgcctgg tggctgctgc agctgcgcgc
     4321 cgagtcggcg cctgccgaca cggccggcag cggcgcggcc gatgcggcca ccgaccaggt
     4381 ggaggtcacc gccagttccg gcggctcgtc catgcattgg cagagcgccc tgcaggg
//
LOCUS       DASZZC010000330        12257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1052222,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000330 DASZZC010000000
VERSION     DASZZC010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12257
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052222"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..437)
                     /locus_tag="VHE37_06925"
     CDS             complement(237..437)
                     /locus_tag="VHE37_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35297.1"
                     /translation="MTGRHFGHRNQYSNGRARLREHDTETIRAPTVCIGPGVLPMQDS
                     SAAIFVTSLSNMDTPLLGHYGL"
     gene            544..972
                     /locus_tag="VHE37_06930"
     CDS             544..972
                     /locus_tag="VHE37_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35298.1"
                     /translation="MARGFSAIQQAVLDALSDKLVVTLSAIDLARKINHGVRSKSIII
                     SVHRALRTLEALGKVVRIEGKWVLATRRREAGRFNRKKGQEPAAHQNERLKLLSKILG
                     MLGSSEDGEVLAAARKAEAKRAELGLSWEMIIMGRTILVD"
     gene            complement(1083..1262)
                     /locus_tag="VHE37_06935"
     CDS             complement(1083..1262)
                     /locus_tag="VHE37_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35299.1"
                     /translation="MKKLERTEDQKPGTVTYELDDGRYVTLEECAVQQYGVKQLLEWL
                     DIERPVAAEKKKGHS"
     gene            complement(1524..1829)
                     /locus_tag="VHE37_06940"
     CDS             complement(1524..1829)
                     /locus_tag="VHE37_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35300.1"
                     /translation="MFPMDKKYVGLDQDIYGITQLGRIVLDARVFGLIPETQDCAGWN
                     IGQMQALKGKIEQLWDQYGNLPSALPPELAERHNRIYAEAIARAKERGWDAELSEDE"
     gene            2032..3729
                     /locus_tag="VHE37_06945"
     CDS             2032..3729
                     /locus_tag="VHE37_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVT35301.1"
                     /translation="MDHWVSWCLVALPTLPALAAIIAPLAGTGRPARPARISVAMLAA
                     TLAIALALLVAVLRAPQGDVALVQLGPAALLLDHLAVAMAVLVAAISLVVHIYAVCYM
                     ADEPGYPRFFALLDLMTAVILLMVVAADLLTLLAAWFLIGVLLFFLLGFDTSRKASGR
                     YALWTFLTYRVGDLPLVGAALLLWHGYGTAQLPELFSRISADPNMHLGEVAVVPVAAM
                     LLALAAFARSAQFLLHTWLPYTMDGPTPVSALMHAGIVNAGGFLFNRFAPVFVHAPHA
                     LHLAFVVGVVTALVGSALMLTQNDVKKSLGYSTMGQMGFMIMECGLGAFSLAVYHLIA
                     HGLFKATLFLNSGNVIGDTRRNDGVPPDDIYTFVVERRPLRAVKVPWLLAALITVLVP
                     ALVLGLSHWLVAEDFFHKQGAVVLLFFGWVTGAQVFFSIHKLPSESPWRSLTMTLLSF
                     LIVVVGYTLIAHAFEAFLYPDPVLRDAIYAAAGINFLAFDILVVFLTVVLVGAWLMTF
                     YARSLEFEGTPGTLWNAIYLNLYTLFSREFYVSDVYTQLARGALAATKRVNIWLRWV"
     gene            3736..4497
                     /locus_tag="VHE37_06950"
     CDS             3736..4497
                     /locus_tag="VHE37_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35302.1"
                     /translation="MIALLLALILLPLFPLSMLFNAGLQMLPVWLRVPALVALPAAGA
                     LLLNQATAPAGQLTTALQLLAAFTALLYAWRLLAVQEVFIWARLQATSAWPLVWLAWL
                     HGLHGTKLVLVALAVTIPAAALMVLGCSLSRRLGGAYLGLRGRIGPTFPRLTGVFVVA
                     LLAALAAPPFPGFFAMFAVLKQVPAGLAAVVLAVWLLWSWAAAVLWQHGLFGDQWPRA
                     AGTADLSRSGAALWLVLAAAAAVLGSIGGWSWWMH"
     gene            4482..7595
                     /locus_tag="VHE37_06955"
     CDS             4482..7595
                     /locus_tag="VHE37_06955"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HVT35303.1"
                     /translation="MVDALNLGRRLRVRSTAYVAGEPVPFFWPMRNFIHHNPLYGLEH
                     MPFEQAVRRGAELFHARMFLPRSNYQHWQREGKVRQDTISEELTRRAQELPAVPGIDW
                     PRWLQALMQSAHDRDAVVPGVREQDVHAALHGKPPSTQAVDVAAQLPALKRRLHALTL
                     PEAVDALWGPRLADELDELVIKSCLDFFDEDQSSWRMPGRERGLFAAWSEIARRNARM
                     FLRGLHVRRILDRVRDAESAVVHVMEEMGIDADDWSAYFTRELTRLHGWTGFVRWRSS
                     AKHYYWAQRYPADVVDLLAVRLVVGLALLQESARHRRTPVRREQLDAVLHDRGAESLL
                     RNALHSGEVLPDWAQRIDDTLSRGGGKRCESLLQRYWPLWQAQRGQEQATTLRELAAA
                     ADATAALDALTPEDVGGLLQGLREFAPQEGMVWTLAMEAESIDRLLAQVQVPEDLPSG
                     KRPFAQAWFCIDVRAEPIRRHLERVGDYQTFGIAGFFGVPVGFLGYGKGSESHFCPAV
                     VTPKNLVLELPAELDPEEDDLLSTLGHVLHDLKSSVLSPYVTVEAVGMLFGLDLFGKT
                     LAPLGYSRWRSRIDAEKPVTRLLVDKLTREHADSIIRTLQRAMIVKALHAELHIERER
                     VDDDMIRELRETALRHREGPTRLQTAFSVPDKQEAEFIDKLREDYRVDADYANYQLVR
                     LGRIGYSLDEQVNYVHTALTMIGLTKTFSRFVLIVGHTGQTENNPYESALDCGACGGA
                     SGLVNARVLAQMANKTEVRERLRGMGINIPDDTWFLPALHITTTDAIELFDLDLLPPR
                     HLVYLDRLRNGLLAASKLAAAERMPKLLSEAQAIEPAQAWRLAHRQAVDWAQVRPEWG
                     LSKNLYGIVGRRSLSEHADLEGRPFLLSYDWRCDPKGRLLENLLAAPVVVGQWINLEH
                     FFSTVDNARMGSGSKAYHNVAGRFGVMTGNLSDLRTGLPMQTVMREGQPYHEPMRMIA
                     LIEAPLDFAGRALQSVVKVKNLVLGGWIRAIVIDPTQGYKPFVFNNGQWEERPPLVAP
                     AKEEHAA"
     gene            7592..7918
                     /locus_tag="VHE37_06960"
     CDS             7592..7918
                     /locus_tag="VHE37_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVT35304.1"
                     /translation="MNAIKLHPLKKLEIILEGAHKEFATDLLDRAGVKGYTIVGNLSG
                     KGSHGMYEGHLMFNEDDALIMIIVAVPQSLFDPLLEGFQPFFEAHAGVIFVSDIQVGR
                     PVKFMA"
     gene            complement(7908..8840)
                     /locus_tag="VHE37_06965"
     CDS             complement(7908..8840)
                     /locus_tag="VHE37_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009561657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT35305.1"
                     /translation="MHLTFRQLQIFDAVVHQQSFTRAADALHLTQPAVSMQMRQLEEQ
                     VGVPLFDHVGKQIQPTDAGRELHRHARLISAKMRDLESAMEEFRGVHRGELDLTVAST
                     ANYFMPKLIAAFCACHAKVQVRLQVSNQTHILESLSAANQDLAIMGQPPEDAELVARA
                     FLDNPLVVIASPEHPLAQHRQIPLMQLGCQPFITREPGSGTRNAAERHFSRHGVEFHS
                     VMEMSSNEAIKQAVEAGLGLGILSLHTLELELAIGRLVTLDVESFPIVRQWFVVHRKG
                     KRLSAVAQSFLAFLTQQARPLASPKHPQLAPLTP"
     gene            9072..10493
                     /locus_tag="VHE37_06970"
     CDS             9072..10493
                     /locus_tag="VHE37_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HVT35306.1"
                     /translation="MAVKTYQAGVTEYRQSYWQPDYAPLDTDILACFKITPQPGVDRE
                     EAAAAVAAESSCGTWTTVWTDLLTDLDYYKGRAYRLEDVPGDDTCFYAFIAYPIDLFE
                     EGSVVNVFTSLVGNVFGFKAVRALRLEDLRFPIAYVKTCGGPPHGIQVERDKLNKYGR
                     PLLGCTIKPKLGLSAKNYGRACYEALRGGLDFTKDDENINSQPFMRWRDRFDFVMEAV
                     QKAEQETGERKGHYLNVTAPTPEEMYKRAEYAKEIRAPIIMHDYLAGGLCANAGLANW
                     CRNNGMLLHIHRAMHAVIDRNPHHGIHFRVLTKILRLSGGDHLHTGTVVGKLEGDRAS
                     TLGWIDLLRESYVPEDRSRGIFFDQDWGSMPGGFAVASGGIHVWHMPALVTIFGDDSV
                     LQFGGGTLGHPWGNAAGAHANRVALEACVQARGEGRHLEKEGKDILTAAAAHSPELKI
                     AMETWKEIKFEFETMDKLAIANK"
     gene            10567..10893
                     /locus_tag="VHE37_06975"
     CDS             10567..10893
                     /locus_tag="VHE37_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HVT35307.1"
                     /translation="MAVQAYRSMKKYETFSYLPQMTPDQVRRQIAHAIAQGWNPAVEH
                     TEKGTPAKASYWYMWKLPLFGEQSVDTVMAEIEACHREFPDQMVRFVAYDNYAQSQGM
                     AFVVYR"
     gene            10997..>12257
                     /locus_tag="VHE37_06980"
     CDS             10997..>12257
                     /locus_tag="VHE37_06980"
                     /inference="COORDINATES: protein motif:HMM:NF023708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043886 - structural constituent of
                     carboxysome shell [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsoS2 family carboxysome shell protein"
                     /protein_id="HVT35308.1"
                     /translation="MTNVAPGHATATLSGRELALQRRQAMARHGKTGTTKAAQTERSA
                     AERFRLNAAHISNAHHGGVSAAPASAPFTQPVAQKSAARARRQALSTIGKAALKNAGH
                     GTATRPAAHLRPGRAAAEVRGSDCGCGCGGQCSCGGQCGCSHGAERKEAARTEAVQHG
                     APAVERAALNAPEATGRALAKMRRAALSQDGKKGLKRVAQATRIAAAMPQQDWQSAML
                     KGATGRQVAMQRRLVQALAGRVDKTSGASTRPTGRVRARNVVEAPAKVEEGHTLSGQT
                     VTGTQVERISKVTGNEPGSCRAITGTEYIGFEQFQTLCGIRPEPATPKVAVSSTLREQ
                     RITGTALGRSTKTTGDEPGACRPVTGTEYLSSERFEQFCSTRPSAPVGKVALTRTDKG
                     QTVTGALVDRPRRVTGVEHGADRAVTGT"
BASE COUNT         2079 a         3865 c         3941 g         2372 t
ORIGIN      
        1 cggttttctg gccagagtat gtcaatgcgg ttcgcacgct tcttaagctc gacgacaagg
       61 atctcggcca gggtctccgg ttctccacct gcgggccgca gcaaacccac gcacagcggc
      121 gaggagactc tcacggacat caactatcag atgaatccgt cagcgcgact atccagcgat
      181 cgctatgcgt acgagaaggc gttgctggac ggctggttcc gccgcgtagt cggtcgtcag
      241 aggccgtaat gtccgagcag cggcgtgtcc atgttggata gtgaggttac aaatatcgca
      301 gcgctggaat cctgcatcgg caaaacgccg gggccgatgc atacagttgg cgcgcggatc
      361 gtttccgtat cgtgctcgcg caaccgcgcg cggccattgc tgtattgatt ccgatgccca
      421 aaatgcctgc ctgtcatgcg ccgggctcga tcgtcggctc gcgaactcgg cgtcaagtgc
      481 tggtggcatt tcgtgccggt aacgcctaaa attagcctgc gcgaatagcg ttggagggtt
      541 cccatggcgc gaggtttcag cgcgattcag caggcggtcc ttgatgcgtt atcggacaag
      601 ctcgtggtca cgctctcggc gattgatctc gctcgcaaga tcaaccatgg ggtccgcagc
      661 aaatcgataa tcatatcggt tcatcgcgcg ttacgaactt tggaagcttt gggaaaagtg
      721 gtccgcattg agggaaagtg ggttttggcg accaggcgaa gggaagccgg acgctttaac
      781 cgcaagaaag ggcaggagcc ggctgctcat caaaacgaac gattgaagct gctttcaaaa
      841 atactcggaa tgctcggttc gtccgaggat ggtgaagttc tggcagcagc gcgaaaggca
      901 gaggccaaga gggccgagtt aggtttgtcc tgggaaatga tcatcatggg ccgtacgatc
      961 ctggtcgact gacgggatgg cctagagtct gtttcaactg cgtcggatca gactcgtcgt
     1021 ttatcttttt gtttgagcat gatcttttcc gaaagccggt tcccactttt cggcatcatg
     1081 ctctaggaat gtcccttctt cttttcggcc gccactggcc gttcgatatc gagccattcc
     1141 agaagttgtt ttaccccgta ctgttgcacg gcgcattcct cgagcgtgac atagcgacca
     1201 tcatctaact catatgtaac ggtccccggc ttttggtcct ccgttcgttc gagctttttc
     1261 atcttccctc ctggtcgaac ggtcagccac gcgcggattg cggcaaacat aaacgccggt
     1321 ggcgcaggct ttgcgggtgt cctctcagat cgcaacgggc tcatacgaac tcgctccgcc
     1381 accgcgagct tcatctaata agcaggcgtt ccggcgtgag ttcctggcgt gatgaatcag
     1441 gcagcagatt cgggagacgc cgcgacatct ggacgccccc actttcgggg cgcttaccgg
     1501 gagcggtatg gcgcggcggc gccctattcg tcctctgaca actcggcgtc ccagccccgc
     1561 tccttggcgc gggcgatggc ctcggcatag atgcggttat ggcgctcggc caactccggc
     1621 ggaagcgcgc tcggcaggtt gccatactga tcccagagct gctctatttt gcccttcagc
     1681 gcctgcatct ggccaatgtt ccagcccgcg caatcctgcg tctccggaat gagaccgaac
     1741 acgcgggcat cgagcacgat gcgcccgagc tgggtgatcc cgtagatgtc ctgatcgagt
     1801 ccaacgtatt ttttatccat ggggaacacg ggaatgagtt gcgggggcag cgattatcgg
     1861 aatgtatcag ttcgccttag ggattgaata agcaattcaa atttttcctt attcaattca
     1921 tctcttatcg tgtggccgtt cagccaaaag gagctggcga acctagccgc tttcgcatcg
     1981 tgccatcccg ttcggcatta tgccatcccg attgagcgag gagtatctac catggaccac
     2041 tgggtctctt ggtgtctcgt ggcgttgccg acgctgccgg cgctggcggc gatcattgcc
     2101 ccgctcgccg gaacaggccg gccggcacga ccggcgcgca tcagcgtggc tatgctcgct
     2161 gccaccctcg cgatcgcatt ggcgttgcta gtggcagtgc tgcgcgcgcc acagggtgac
     2221 gtggcgctgg tgcaactcgg cccggcggct ctactgctcg accatcttgc cgtggcgatg
     2281 gcggtgctgg tagcggctat cagccttgta gtgcacatat atgcggtttg ctatatggcc
     2341 gacgaacccg gctacccgcg gtttttcgcc ttgctcgacc tgatgaccgc cgtgatctta
     2401 ctgatggtgg tcgccgccga tctgctgacg ttgctcgccg cctggttcct gatcggagtg
     2461 ctgctgttct tccttctggg gttcgacacc agccgcaagg ccagcggccg ctatgccttg
     2521 tggacattcc tgacctatcg cgtgggcgat ctgcctttgg tgggcgcagc gctcctgttg
     2581 tggcatggct atggcaccgc gcagctgccg gagctgttct ctcggatcag cgccgacccg
     2641 aacatgcatc tcggcgaggt ggccgtggtg ccggtggcgg cgatgctgct ggcgctggcg
     2701 gcgtttgccc gctcggcgca gtttctgctg cacacttggc taccttacac catggacggt
     2761 ccgacgccgg tgtccgcgct gatgcatgcg ggcatcgtca acgctggcgg attcttattc
     2821 aaccgcttcg ctccggtatt tgtccatgcg ccccacgcgc tgcacctagc cttcgtcgtc
     2881 ggagtcgtca cggcgctggt cggatcggcg ctgatgctga cgcagaacga cgtcaaaaaa
     2941 tccttgggct attccaccat ggggcagatg ggcttcatga tcatggaatg cgggctgggc
     3001 gcgttttcgc tcgcggtcta tcacctcatc gcccacggtc tgttcaaagc caccctgttt
     3061 cttaattcgg gcaacgtgat cggcgacacc cgtcgcaacg atggcgtgcc gcccgacgac
     3121 atctacacct ttgtggtcga gcgtcgtccg ctgcgtgcag tgaaggtgcc ctggctgttg
     3181 gccgcgctca tcaccgtgct ggtgcctgcg ctggtgctgg gcctgtcgca ctggctggtg
     3241 gcggaggact tcttccacaa gcagggcgcg gtggtgttgc tattcttcgg ctgggtgacg
     3301 ggagcgcagg tattcttctc cattcacaag ctcccgagcg aaagcccctg gcgctcgctg
     3361 accatgaccc tcttatcctt cctgatcgtg gtggtgggct acacgctgat cgcgcatgcg
     3421 ttcgaggctt tcctctaccc ggatccggtg ttgcgcgatg ccatctacgc tgccgcaggc
     3481 atcaacttcc tggctttcga tatcctggtg gtcttcctta ccgttgtgct ggtcggggcc
     3541 tggctcatga cgttttatgc ccggtccctc gaattcgagg gcacgcccgg cacgctgtgg
     3601 aatgccatct acctcaatct ctatacgttg ttttcgcggg agttttatgt gtccgacgtt
     3661 tatacccaac tggcgcgagg cgcgctggcc gcgactaagc gggtgaatat ctggctgcgt
     3721 tgggtctgaa ccgccatgat cgcgctgctc ctcgccttga tcctgttgcc gctgtttccc
     3781 ctgagcatgt tgttcaatgc ggggcttcag atgctgcccg tgtggctgcg cgttccggcg
     3841 ctagtggctc ttcccgctgc gggcgcgctg ctactcaacc aggcgaccgc gccggccggg
     3901 caactcacaa ccgcgctgca gctcttggcg gcgttcaccg cgctgcttta tgcttggcgg
     3961 ctgctcgcgg tccaggaggt gttcatctgg gcccgtctgc aggccacctc ggcatggcct
     4021 cttgtctggc tggcctggct gcatggcctc catggcacga agctcgtgct ggtagctttg
     4081 gcagtcacaa ttcccgctgc ggcactgatg gttctcggct gcagcctgag ccgccgcctc
     4141 ggaggggcgt atctcggact gcgcggacgc atcgggccga cctttccgcg tctgaccggc
     4201 gtgtttgtgg tggcgttgct tgcggccctg gcggcgccgc ccttccccgg tttcttcgcc
     4261 atgttcgcgg tgctgaagca ggttcccgcg ggtctcgcgg cggtggtgct cgcggtgtgg
     4321 cttttgtgga gctgggcggc cgcggtgctg tggcagcacg gcctgttcgg ggaccaatgg
     4381 ccacgcgcag ccggcacggc tgacctgtca cgcagcggcg cagccctgtg gctggtgctt
     4441 gctgctgccg ccgcagtgct gggttcgatc ggaggatggt catggtggat gcattgaacc
     4501 tggggcggcg tctgcgagtg cgttcgaccg cctacgtggc gggcgagccg gtgccatttt
     4561 tctggccgat gcgcaacttc attcaccaca acccgctcta cggccttgag catatgcctt
     4621 tcgagcaggc cgtccgccgc ggtgcggagc tgttccacgc gcgaatgttc ctgccgcgca
     4681 gcaactacca gcactggcag cgcgagggca aggtgcgcca ggataccatt tccgaggagc
     4741 tcacgcgccg cgctcaggaa ctgcccgccg tgccgggcat cgactggcct cgctggctgc
     4801 aggcgctgat gcagtccgcg cacgaccgcg atgctgtggt gccgggcgtg cgcgagcagg
     4861 atgtccacgc cgcgctgcat ggaaagccac catccaccca ggcggtggat gtggccgcgc
     4921 agctgcccgc actcaagcgg cgcctgcacg cgctgacgct gcccgaggcg gtcgacgccc
     4981 tatggggccc gcggctggcc gacgaacttg atgaactagt catcaagagc tgcctcgact
     5041 ttttcgacga ggaccagtct tcctggcgca tgccgggacg cgagcgcggc ctgttcgccg
     5101 cctggagcga gatcgcgcgg cgcaatgcgc gcatgttcct gcgcggcctg cacgtgcgac
     5161 gcatcctcga tcgggtgcgg gacgccgaaa gcgcggtggt gcacgtcatg gaggagatgg
     5221 gcatcgatgc cgacgactgg tcggcctact tcacccgcga gctcaccagg ctgcacggct
     5281 ggactggctt tgtgcgctgg cgttcgtccg ccaagcatta ttactgggcg cagcggtacc
     5341 ccgccgacgt cgtcgatctt ctggccgttc gcttggtggt gggattggcg ctcctgcagg
     5401 agagcgcgcg gcatcgccgt acgccggttc ggcgcgagca gctcgacgcc gtactgcacg
     5461 accgcggcgc agagagcctg ctgcgcaatg cgttgcacag cggcgaagtg ttgcccgact
     5521 gggcgcagcg catcgacgac accttgtcgc gcggcggcgg caaacgctgc gagagtctgc
     5581 tgcaacgcta ctggccgcta tggcaggccc agcgcggaca ggagcaggcc accacgcttc
     5641 gcgaactggc tgccgccgcc gatgccacgg cggcgctcga tgcgctaacg ccggaagacg
     5701 tcggtgggct attgcagggc ttgcgcgaat tcgcgcccca ggagggcatg gtctggaccc
     5761 tggcgatgga ggcggaatcc atcgaccggc tgctcgcgca ggtgcaagtg cccgaggatc
     5821 tgccgtccgg caagcggccg ttcgcgcagg cttggttctg catcgacgtg cgcgccgagc
     5881 ccatccgtcg ccacctggaa cgcgtcggcg actaccagac gttcggcatc gctggctttt
     5941 tcggcgtgcc cgtgggcttc ctcggctatg gcaagggcag cgagagtcac ttctgtccgg
     6001 cggtggtcac gcccaagaat ctggtgctgg aactgcccgc cgaactcgat ccggaagagg
     6061 acgacctgct cagcacgctc ggtcacgtgc tgcacgatct caagagttcg gtgctttcac
     6121 cttacgtcac ggtggaggcg gtgggcatgc tgttcgggtt ggacctgttc ggcaagaccc
     6181 tggcaccact gggctatagt cgctggcgca gccgcatcga tgccgaaaag cccgtcaccc
     6241 gcctgttggt ggacaagctc acccgcgagc acgcggactc catcatccgc acgctgcagc
     6301 gcgcgatgat cgtcaaggcg ctgcatgccg aattgcatat cgagcgcgag cgggtggatg
     6361 acgatatgat ccgcgaactg cgcgaaaccg cgttgcgtca ccgtgaaggt ccgacgcggc
     6421 tgcaaacggc cttcagcgtg ccggacaagc aggaggccga attcattgac aaactgcgtg
     6481 aggactaccg cgtcgatgct gactacgcca attatcagct cgtgcggcta gggcgcatcg
     6541 gctattcgct cgacgagcag gtcaattacg tgcacacggc cctgaccatg atcgggctga
     6601 ccaagacctt ctcgcgcttc gtgctcatcg tgggccacac cgggcagacc gagaacaacc
     6661 cctacgagtc ggcgctggat tgcggcgcct gcggcggcgc cagcggcctg gtcaacgccc
     6721 gggtgctggc gcagatggcc aataagaccg aggtgcgcga gcggctacgc ggcatgggca
     6781 tcaacattcc cgatgacacc tggtttctgc cggcgctgca catcaccacc accgacgcca
     6841 ttgagctgtt cgacctcgac ctgctgccgc cgcgtcacct ggtctatctg gatcgactgc
     6901 gtaatggcct gctcgccgcc tccaagctcg ccgcggccga gcgcatgccg aagcttctgt
     6961 ccgaggcgca ggcgatcgaa ccggcgcagg cctggcgcct ggcacaccgt caggcggtgg
     7021 actgggccca ggtacggccg gagtgggggc tatcgaaaaa cttgtacggc atcgtcggcc
     7081 ggcgctcgct cagcgagcat gccgacctgg aggggcggcc cttcctgctg tcgtatgact
     7141 ggcgctgcga tcccaaggga cgcctgctgg aaaacctgtt ggcggcgccg gtggtggtag
     7201 gccaatggat caacctcgaa cacttcttct ccaccgtgga caatgcccgg atgggcagcg
     7261 gcagcaaggc ctatcacaac gtggcgggac gcttcggcgt gatgaccggt aacctgagcg
     7321 atctgcggac cggcctgccg atgcagacgg tgatgcggga gggtcagccc tatcacgagc
     7381 ccatgcgcat gatcgccctc atcgaggcgc cgctggattt tgccgggcgc gcgctgcaaa
     7441 gcgtggtcaa agtcaagaac ctggtgctcg gtggctggat ccgcgccatc gtcatcgatc
     7501 ccacacaggg ctacaagcct ttcgtcttca acaatggcca gtgggaggag cgcccgcctc
     7561 tagtcgctcc tgccaaggag gaacacgccg catgaacgcc atcaagctgc atccactgaa
     7621 aaagctggaa atcattctcg aaggcgcgca caaggaattc gccaccgact tgctcgaccg
     7681 cgccggagtc aagggctaca ccatcgtcgg caacctgtcg ggcaagggca gccacggcat
     7741 gtacgaaggg cacctcatgt tcaatgagga tgacgccctc atcatgatca tcgtcgccgt
     7801 gccgcaaagc ctgttcgatc cgctgcttga gggctttcag ccgtttttcg aggcccatgc
     7861 gggggtgatt ttcgtcagcg acatccaggt cgggcgtccg gtcaagttca tggcgtgagc
     7921 ggagcgagct gcggatgctt gggcgaggcc agcgggcgcg cctgctgggt gaggaacgcc
     7981 aggaaggatt gggccacggc tgacagccgc ttgcccttgc ggtgcacgac aaaccattgc
     8041 cgcacgatcg gaaagctctc cacgtcgagg gtgaccaggc ggcctatcgc cagctccagc
     8101 tccagggtgt gcagcgacag gatgcccaac cccagcccgg cttcgaccgc ctgcttgatg
     8161 gcctcgttgc tgctcatctc catcaccgag tggaattcca cgccgtgacg cgaaaagtgc
     8221 cgctctgcgg cattgcgcgt gcccgagccg ggctcgcggg taatgaaggg ctggcaaccc
     8281 aattgcatca gcggaatctg ccggtgttgg gccaggggat gctcgggtga ggcgatgacc
     8341 accagcgggt tgtcgaggaa cgcgcgcgcg acgagctcgg cgtcctccgg cggttgtccc
     8401 atgatggcca ggtcctggtt cgccgccgag agcgactcga ggatatgcgt ctggttggac
     8461 acctgcagcc gcacctgcac cttggcgtgg caggcgcaga aggctgcaat cagtttgggc
     8521 atgaagtaat tggccgtgct cgccacagtg aggtcgagtt ccccgcgatg gacgccgcgg
     8581 aactcctcca tcgcggattc aaggtcgcgc atcttggcgc tgatgagtct ggcatggcgg
     8641 tgcaactcgc gaccggcgtc ggtcggctgg atctgttttc cgacgtggtc gaacagcggc
     8701 acgccgacct gctcttccag ttgtcgcatc tgcatcgaga ccgccggttg cgtcagatgc
     8761 agcgcgtcgg ccgcgcgcgt gaagctctgc tgatgcacga ctgcgtcgaa gatctggagc
     8821 tgtctgaagg tcaggtgcat ggctgataca taagcgagtc taatgtatct gatcaataat
     8881 aacaattagt ttttatgcag agcctctttt atgttgggca ctcgtgaatc gcggggccgt
     8941 gctgaatgac aggccctgct gagatgcacg aacgtaatcc ccgggccggg tggggggatg
     9001 gccgccgcgt cgcggtaggt cctcgcccgt cgttcgctac cgcaaaagcg cgcatgaagg
     9061 agtcctcgaa gatggcagtc aaaacctacc aggccggcgt cacggaatat cggcaaagct
     9121 actggcagcc ggattacgcg cctctcgaca ccgatatctt ggcctgtttc aagatcacgc
     9181 cgcagcccgg ggtggatcgc gaagaggcgg ccgcggcggt cgccgccgag tcgtcctgcg
     9241 gcacctggac cacggtgtgg accgatctgc tgaccgacct cgactactac aagggacgcg
     9301 cctatcgcct cgaggacgtg ccgggcgacg atacgtgctt ttacgctttc attgcctatc
     9361 ccatcgacct tttcgaggaa ggctcggtgg tcaacgtgtt cacctcgctg gtgggcaacg
     9421 tgttcggatt caaggctgtc cgcgcgctgc gcctggagga tctgcgcttc cccatcgctt
     9481 acgtgaagac ctgcggtggc ccgccccacg gcattcaggt cgagcgcgac aagctcaaca
     9541 aatatggccg tccgctgctg ggctgcacca tcaagcccaa gctcggcctg tccgccaaga
     9601 actacggccg cgcctgctac gaagccctgc gtggtggtct ggacttcacc aaggacgacg
     9661 agaacatcaa cagccagccg ttcatgcgct ggcgcgaccg ttttgatttc gtgatggagg
     9721 cggtccagaa agccgaacag gaaaccggcg agcggaaggg ccactatctc aacgtgaccg
     9781 cgcctacgcc cgaggaaatg tacaagcgcg ccgagtatgc caaggaaatc cgcgctccga
     9841 tcatcatgca cgattacctc gccggcggtc tttgcgccaa tgcgggtctg gcgaactggt
     9901 gccgcaacaa cggcatgctg ctgcacatcc accgcgccat gcacgcggtg atcgaccgca
     9961 acccgcacca cggcattcac ttccgcgtgc tgaccaagat cctgcgtctg tcgggtggcg
    10021 atcatctgca caccggcacc gtggtcggca agctcgaggg cgaccgcgcc tccaccctgg
    10081 gctggatcga tctgctgcgc gaatcctacg ttcccgaaga ccggtcgcgc ggcatcttct
    10141 tcgatcagga ctggggttcg atgcccggcg gtttcgccgt tgcatccggc ggtattcacg
    10201 tttggcacat gcctgcgctg gtcaccatct tcggcgacga ctcggtgctg cagttcggcg
    10261 gcggcacgct cggtcacccg tggggcaatg cggccggtgc gcacgccaac cgtgtcgcgc
    10321 tcgaagcttg cgtccaggcg cgcggcgaag gccgtcatct ggagaaggaa ggcaaggaca
    10381 tcctcaccgc ggccgccgcg cattcgcccg aactgaagat cgccatggaa acatggaaag
    10441 agatcaagtt cgagtttgaa accatggaca agctcgccat cgccaacaag taaccgccgg
    10501 ctgcggtcgg cagcgcctgc cgggcgcgcc accgcccagc agaaccactt tcgaaaggac
    10561 tagatcatgg ccgttcaagc ctaccgctcg atgaaaaagt acgagacctt ctcctatctg
    10621 ccgcagatga cgcccgatca ggtgcgccgt cagatcgctc acgccatcgc gcagggttgg
    10681 aatccggcgg tcgagcacac cgaaaagggc acgcccgcaa aggcgagcta ctggtacatg
    10741 tggaaactgc cgctgttcgg cgagcagtcc gtcgataccg tgatggcgga gatcgaggcc
    10801 tgtcaccgcg aatttccgga tcagatggtg cggttcgtcg cgtacgacaa ctacgcccag
    10861 agccagggca tggcattcgt ggtgtatcgc tgatgagctg ggcgctggag cctgctctgt
    10921 tcggcgccca tgatttgaac gcggcgcggc gcatcggcgc cgctcccttc ctcgggttct
    10981 ttgtgctgag gtccgtatga caaacgtcgc ccccggtcat gccactgcaa ctctatccgg
    11041 ccgtgaactg gcgctccagc gccggcaggc catggcgcgt cacggcaaga ccgggaccac
    11101 caaggctgcg caaaccgagc gctccgccgc ggaacgcttt cggctgaatg cggcgcatat
    11161 ctccaacgcc catcacggcg gcgtttcggc ggcgcctgcg tcagcgccgt tcactcagcc
    11221 ggtggcgcag aagtccgcgg cgcgcgcccg gcggcaggcc ttgtccacta ttggcaaggc
    11281 ggcgttgaaa aatgccgggc acggcacggc aacccgcccc gcggcgcatc tgcgcccagg
    11341 ccgggcggcg gccgaggtgc gtggctccga ttgcggttgc ggctgcggcg gacagtgcag
    11401 ttgcggcggg caatgcggct gcagccatgg cgcggagcgc aaggaggcgg cgcgtaccga
    11461 agctgttcag cacggcgcac cggcggtgga gcgtgcagcg ttgaacgcgc cggaggcgac
    11521 cggccgcgcg ctggccaaga tgcgccgtgc cgcgctgagt caggatggca agaagggcct
    11581 caagcgcgtc gcccaggcaa cacgcatcgc tgccgcgatg ccgcagcagg attggcagag
    11641 cgccatgctc aaaggcgcaa ccggccgtca ggtcgcgatg cagcgccggc tggtacaggc
    11701 gctggcgggc cgggtcgata agacgtccgg tgccagcact cgcccgaccg gccgtgtgcg
    11761 cgcccgcaac gtggtcgagg cgccggccaa ggtcgaggag gggcataccc tgtccgggca
    11821 gaccgtcacc ggcacccagg tcgaacgcat ctccaaggtg accggcaatg agccgggctc
    11881 ctgccgcgcg atcaccggca ccgaatatat cggcttcgag cagttccaga ccctctgcgg
    11941 catccgcccc gagccggcga cgccgaaggt cgcggtcagc agcaccctgc gcgaacaacg
    12001 catcaccggc acggcgctgg gccgttccac caagaccacc ggcgatgagc cgggcgcgtg
    12061 ccgccccgtg accggtaccg aatatctctc gagcgaacgc ttcgagcagt tctgttcaac
    12121 gcggccgtcc gccccggtgg ggaaggtggc gctcacccgc accgacaagg gccagactgt
    12181 caccggcgcg ctggtcgatc gcccgcgccg cgtcacgggc gtggagcacg gcgccgaccg
    12241 cgccgttacc gggacgc
//
LOCUS       DASZZC010000331         6376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_992094,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000331 DASZZC010000000
VERSION     DASZZC010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992094"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /gene="sufB"
                     /locus_tag="VHE37_06985"
     CDS             complement(<1..1017)
                     /gene="sufB"
                     /locus_tag="VHE37_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HVT35309.1"
                     /translation="MSSTLESLVKRDYSAGFVTEIDTETVPPGLSEDTIRYISAKKNE
                     PAWLLDWRLKAYARWQKMSEPTWAHVHYPPVDYQAISYYSKPKSVKDAPKSLDEVDPK
                     LLETYKKLGIPLKEQEMLAGVAVDVVFDSVSVATTFRTRLKEAGVIFCPISEAVREHP
                     ELVQKYLGSVVPQGDNFYAALNSAVFTDGSFVFVPKGVKCPMDLSTYFRINERNTGQF
                     ERTLIVCEEGASVSYLEGCTAPQRDENQLHAAVVELVALDDATIKYSTVQNWYPGDEN
                     GKGGIYNFVTKRGDCRGARSKISWTQVETGSAITWKYPSCILEGDNSVGEFYSVALTN
                     NRQQA"
     gene            complement(1014..1460)
                     /locus_tag="VHE37_06990"
     CDS             complement(1014..1460)
                     /locus_tag="VHE37_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HVT35310.1"
                     /translation="MLKLSKLSDYATVLITAMAHQPGHTWNTQELGARAHVSAPTAAK
                     LLKLLARAGLIESIRGAHGGYRLAREPAQISVADIIAAVEGPIAVTECAQHGGGCSIE
                     SSCATRSNWRLINNAIRQALAAVTLAQMAAPMRRSESPIHFHKSAA"
     gene            1535..2512
                     /locus_tag="VHE37_06995"
     CDS             1535..2512
                     /locus_tag="VHE37_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35311.1"
                     /translation="MLADYNHARFDPASAAGHYESFFVRANHPSRPLAFWIRYTVFAP
                     RGRPQDAIGELWAVVSDGESGKIAVAKQELPIARCEFARDRFFSRVGDAVQQDGRVQG
                     QAGDIRWQLIYEGAGAPLLMLPEQLYAAKLPRAKTLTGAPMAVFTGTLAVGGNTIAVD
                     GWRGSQNHNWGSRHTDRYAYGQVCGFDNAPDSFLELASARLKFGPLWTPLMTPLVLRH
                     AGREHALNGIGRSLRARARFEYFEWTFSGEGDGVGIDGWMRARREDFAGLRYYNPPGG
                     AKYCLNSKIADCDLTLRHADGRIETLVASRRAAFEILTDDLQHGVPIMA"
     gene            complement(2502..4289)
                     /locus_tag="VHE37_07000"
     CDS             complement(2502..4289)
                     /locus_tag="VHE37_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35312.1"
                     /translation="MLPSPWPARLAPLLPFLVACLLYWPCTHFEFIWDDSSYIVGQGA
                     LADWTQAWHSAWSAYLQGSLIYFRPLPILTLALDILPDGAAAAAHRSNVLLHAANSAL
                     VAILSQQLAMHFGHGRRSAAAAALLAGILYAAHPALIQTAAWISCRFDLLLTLLALAF
                     LLADRRLAGKPQRAPGLALLFLLAALCKESAVVLPLLLPWWRRLFAPAGTPLRQQISG
                     ADFSWLLASGCCYLALRAFALHEFDAVRLPWLEPAQQLLLVLKSIGGYAALALLPFGR
                     LDPLHPLLLPVSPLDPEVLAGITTLVATAWALRSSRPLAALLPCWTLALLPVINLLRF
                     NLADYEQERFLTLPLVFVCIGAAQLMLAAVARGGAMRGGSLAVVAGWLAAAVLAIASY
                     LPAWRSNLDLWGYALTHHPASPVAQKFALISLNAAGRSADAAELGLQLDHHYSQLTPE
                     VRFGYARSLLEVGQPAEALRQLDLIAQAHGAEEQWHDSVCAEGGWAALSAGFPAQAES
                     MLRAVSQSRADLAEVRYQLGIALAVQGRGADAQREFERAEQAAPQAAHDWHARAPALI
                     AHATSLAQEEHPPIAHPALDQLCRPLLTP"
     gene            4368..4805
                     /locus_tag="VHE37_07005"
     CDS             4368..4805
                     /locus_tag="VHE37_07005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT35313.1"
                     /translation="MSAPHWDVPEPYTERVTVMPGHHDRFGHTNNVVYLQWLEQVAWA
                     HSQSLGLTFEDYERLGAGCVARRHELEYLAPTFAGDELLLGTWIDQNDGRLDMWRAYQ
                     IVRAGDGRTVLRGRTRWVCVDLKTGKPRRQPPEFVERYRPAAK"
     gene            complement(4878..5747)
                     /locus_tag="VHE37_07010"
     CDS             complement(4878..5747)
                     /locus_tag="VHE37_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT35314.1"
                     /translation="MNIGKLHAAAALFGAALASGCQVWPTVNPNVTQARADLDALKSD
                     PQLANRAPAAVADADNAVRVAEQAWTRSDENAGHLGYIADRKVGIARAEAEDRLASDQ
                     MTALAQQRDDLQVAARERQAEFARNEVEQARRDAYYSQAQAQSARQQADIAMMEANDY
                     KQQLQDLQAQQTDRGMVVTLGDLLFATGRANLPAGNQSRLQKLALFLDKNPDRTVIIE
                     GHTDNVGSEQTNQRLSLRRAEAVRSYLVANGVSADRIKAVGKGEDEPVADNGDAAGRQ
                     QNRRVEVIIQTAS"
     gene            complement(5765..6007)
                     /locus_tag="VHE37_07015"
     CDS             complement(5765..6007)
                     /locus_tag="VHE37_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35315.1"
                     /translation="MKEASDKLAQAQSIAAYRDAGMDGVKSSRQLAEEARIDAELATA
                     KSVTARQQLANAEMQKTIDVMRFETQQPESGPYHGN"
BASE COUNT          989 a         2075 c         2236 g         1076 t
ORIGIN      
        1 cgcctgctgg cggttgttgg tcagcgccac cgagtagaat tcacccaccg agttgtcgcc
       61 ctccaggatg cagctcgggt atttccaggt gatggccgag ccggtctcga cctgcgtcca
      121 ggaaatcttg ctgcgcgcac cgcggcagtc gccacgcttg gtgacgaagt tgtagatgcc
      181 gcccttgccg ttctcgtcgc cggggtacca gttctgcacg gtcgagtact tgatggtggc
      241 gtcgtccagc gccaccagct ccaccaccgc cgcgtgcagc tggttctcgt cgcgctgcgg
      301 cgcggtgcag ccttcgaggt acgacacgct ggcgccttcc tcgcacacga tgagcgtgcg
      361 ctcgaattgc ccggtgttgc gctcgttgat gcggaagtac gtcgacagat ccatcgggca
      421 cttcacgcct ttgggcacga acacgaaaga accgtcggtg aataccgcgg agttcagcgc
      481 ggcgtagaag ttgtcgccct gcggcaccac gctgccgagg tatttctgca ccagttcagg
      541 gtgctcgcgc acggcctcgc tgatcgggca gaaaatcacg ccagcctctt tcagcctggt
      601 gcggaacgtc gtggccaccg acaccgagtc gaacaccacg tcgacggcga cgccggccag
      661 catctcctgc tccttgagcg ggatgccgag cttcttgtag gtctccagca gtttcggatc
      721 gacctcgtcg agcgacttgg gcgcgtcctt caccgatttg ggcttgctgt agtacgagat
      781 cgcctggtag tcgactggcg ggtagtgcac gtgcgcccag gtcggctcgc tcatcttctg
      841 ccagcgcgcg taggctttga ggcgccagtc gagcagccag gccggctcgt tcttcttcgc
      901 tgagatgtag cggatggtgt cctcggacag gccgggcggc accgtctcgg tgtcaatctc
      961 ggtgacgaag cctgcgctgt agtcgcgctt gaccaggctt tccagcgtgc tgctcatgcg
     1021 gcgctcttgt ggaaatggat cggcgattcg ctgcgccgca tcggcgcggc catctgcgcc
     1081 agcgtcactg ccgccagcgc ctgacggatg gcgttgttga tcagccgcca gttgctgcgg
     1141 gtggcgcagc tgctctcgat gctgcagccg ccgccgtgct gggcgcattc ggtcaccgcg
     1201 atcgggcctt ccactgcggc gatgatgtcg gccacgctga tctgcgcggg ttcgcgcgcc
     1261 agccggtagc cgccatgcgc gccgcggatg gattcgatca gccccgcgcg cgccagcagc
     1321 ttcaacagct tggccgcggt gggcgccgac acatgcgcgc gcgcaccgag ctcctgcgta
     1381 ttccaggtgt gcccgggctg gtgcgccatg gcggtgatca gcaccgtggc gtaatcggag
     1441 agcttggaca gcttgagcat gaaaataaga ccaaatcagt cctgattagt ttactgcgcc
     1501 gtggctggcg cgggcaaggc agaatggcgg cgccatgctg gccgactaca accatgcgcg
     1561 tttcgatccg gcatctgccg ccgggcacta cgaaagtttc ttcgtgcgcg ccaaccaccc
     1621 atcgcggccg ctggcgttct ggatccgcta cacggtgttc gcgccgcgcg gccggccgca
     1681 ggacgccatc ggcgaactgt gggccgtggt gtccgacgga gagagtggga agattgcggt
     1741 ggccaagcag gaattgccga ttgcccggtg cgaatttgcg cgggatcgct ttttttcgcg
     1801 cgtgggcgac gccgtgcagc aggacggccg cgtgcagggg caggcgggcg atatccgctg
     1861 gcagctcatc tacgagggcg cgggagcgcc gctgctgatg ctgccggagc agctgtacgc
     1921 ggcaaaactg ccgcgggcca agacgctgac cggtgcgccg atggccgtgt ttaccggcac
     1981 gcttgccgtc ggcggcaata ccattgcggt cgacggctgg cgcggcagcc agaaccacaa
     2041 ctggggttcg cgccataccg atcgctatgc gtacggccag gtgtgcggct tcgacaacgc
     2101 gcccgattcg ttcctcgagc tggccagtgc gcggctgaaa ttcggcccgc tgtggacgcc
     2161 gttgatgacg ccgctggtgc tgcgccacgc gggccgcgaa cacgcgctca atggcatcgg
     2221 ccgcagcctg cgcgcccgcg cgcgttttga atatttcgaa tggactttca gcggcgaggg
     2281 cgatggcgtg ggcatcgatg gctggatgcg tgcgcggcgc gaggactttg ccggcctgcg
     2341 ctactacaac ccgccgggcg gtgcgaagta ctgcctgaat tccaagatcg ccgattgcga
     2401 cctgaccctg cgccacgccg acggccgcat cgagacactg gtggctagcc gccgtgccgc
     2461 cttcgaaatc ctcaccgacg acctgcagca cggcgtgccg atcatggcgt gagcaatggc
     2521 cggcacagct ggtcgagtgc cggatgtgcg atcggcgggt gctcctcctg cgccagggac
     2581 gtggcatggg caatcaacgc aggcgcgcgc gcgtgccagt cgtgcgcggc ctgcggtgcc
     2641 gcctgttccg cccgctcgaa ttcgcgctgc gcgtcggcgc cgcgcccctg cacggccagt
     2701 gcaatgccca gctggtagcg cacctcggcc aggtcagcgc gcgactgcga cacggcgcgc
     2761 agcatgctct ccgcctgagc cggaaatccg gccgagagcg ccgcccagcc accctccgcg
     2821 cacacgctgt cgtgccactg ttcctcagcg ccgtgggcct gcgcgatcag gtcgagctgg
     2881 cgcaacgctt cggccggctg gccaacctcg agcaggctgc gcgcgtaacc gaaacgcact
     2941 tccggcgtga gctgtgaata gtggtgatcc agttgcagcc ccagttccgc ggcgtcggcg
     3001 gaacggcccg ccgcgttcag gctgatcagg gcgaatttct gcgccaccgg cgatgccgga
     3061 tggtgcgtca atgcatagcc ccaaaggtcg aggttgctgc gccaggcggg caggtaggac
     3121 gcaatggcca ggacggccgc ggcgagccag cccgccacca ccgcaagcga gcccccacgc
     3181 attgcaccgc cgcgtgcaac ggcagccagc atgagctgtg ccgcgccgat acaaacgaag
     3241 accaggggca aggtgaggaa gcgctcctgc tcgtagtcgg ccaggttgaa gcgcaggaga
     3301 ttgatgacgg gcagcagtgc gagagtccag catggcagca gcgctgcgag cggccgcgac
     3361 gagcgcagcg cccaggcggt ggcgaccagc gtggtgatgc cggccagcac ttcgggatcg
     3421 agtgggctga ccggcagcag cagcggatgc agcgggtcca gccggccgaa cggcagcagc
     3481 gccagcgcgg catagccgcc gatgctcttg agcaccagca gcagttgctg cgcgggttcc
     3541 agccagggca ggcgcaccgc gtcgaattcg tgcagcgcga aggcgcgcag cgcgaggtag
     3601 cagcagcccg aggccagcag ccaggagaaa tccgccccgg aaatctgctg gcgcagcggc
     3661 gtgcccgccg gtgcaaacag ccgccgccac cagggcagca gcagcggcag caccacggcg
     3721 ctttccttgc acagcgcagc cagcaggaac agcagtgcca gtcccggtgc ccgctgcggc
     3781 ttgccggcga ggcgccgatc ggccagcagg aaagccagcg ccagcaaggt cagcagcaga
     3841 tcgaagcggc aggagatcca cgccgcggtc tggatcagcg ccggatgggc cgcgtataga
     3901 atgccggcga gcagggcggc agccgcggcg ctgcgccggc cgtgtccgaa gtgcatggcc
     3961 agctgctgcg acaggatggc gaccagcgcg ctgttcgcgg catgcagcag tacgttgctg
     4021 cggtgcgctg ccgcagccgc gccgtcgggc aggatgtcga gcgccagtgt caggatcggc
     4081 agcggacgga agtagatcag cgatccctgc aggtaggccg accaggctga atgccaggcc
     4141 tgggtccagt cggcgagcgc cccttggccc acgatgtagg aggaatcgtc ccagatgaat
     4201 tcaaagtgcg tgcagggcca gtacagcagg caggccacca ggaatggcag cagcggcgcc
     4261 agccgggctg gccatggaga cgggagcatc agggaggccc gggctgggat cggatcgggc
     4321 gcattgtcgt caagtgccgt gcgcggcaca acgcagcggg ggcgtgcatg agcgcgccgc
     4381 actgggatgt gccggagccg tacaccgagc gcgtgacggt gatgcccggt catcacgacc
     4441 gcttcggtca taccaacaac gtggtttatc tgcagtggct ggaacaggtg gcctgggcgc
     4501 attcgcagag cctgggcctg accttcgagg actacgaacg cctcggtgcc ggctgcgtgg
     4561 cgcgccggca cgagctggaa tacctggcgc cgacttttgc cggcgacgaa ctgctgctgg
     4621 gcacctggat tgaccagaac gacggccgcc tcgacatgtg gcgcgcctac cagatcgtcc
     4681 gcgcgggtga cggtaggacc gtgctgcgcg gccgcacgcg ctgggtgtgc gtggatctga
     4741 agaccggcaa gccgcgccgc cagccgccgg aattcgtcga gcgctaccgg ccggctgcca
     4801 aataaaatcg gcccgcactc cggtgcgggc cgtggcctgc gccgagcgca ggcggactgc
     4861 ggggttctat ccccgcatta cgaggcggtc tggatgatca cttccacgcg ccggttctgc
     4921 tggcggcccg cggcatcgcc gttgtcggcc acgggctcgt cctcgccctt gccgaccgcc
     4981 ttgatgcggt cggccgacac gccgttggcg acgagatagc tgcgcaccgc ttcggcccgg
     5041 cgcagcgaca gacgctggtt ggtctgttcg ctgccaacgt tgtcggtgtg accctcgatg
     5101 atcaccgtgc ggtccgggtt cttgtcgagg aacagcgcca gcttctgcag gcggctctgg
     5161 ttgccggccg gcaggttggc gcggccggtg gcgaagagca ggtcacccag tgtcaccacc
     5221 atgccgcgat cggtctgctg cgcctgcagg tcctgcagct gctgcttgta gtcgttggct
     5281 tccatcatgg caatgtcggc ctgctggcgg gcggattggg cctgtgcctg cgagtagtag
     5341 gcatcgcgcc gcgcctgctc gacctcgttg cgcgcaaact cggcctggcg ttcgcgcgca
     5401 gcgacctgca ggtcgtcgcg ctgctgcgcc agtgccgtca tctggtcgga ggccaggcgg
     5461 tcttcggcct cggcccgcgc gatgcccacc ttgcggtcag cgatatagcc aaggtggccg
     5521 gcgttctcgt cggagcgggt ccacgcctgc tcggccacgc gcacggcgtt gtcggcgtcg
     5581 gccacggcgg ccggtgcacg gttggccagc tgcgggtcgg acttcagcgc gtccaggtct
     5641 gcacgcgcct gcgtcacgtt gggattgacc gtcggccaca cctggcagcc ggaggcgagc
     5701 gctgcgccga acagggcagc ggccgcatgc aattttccga tgttcatagt tgggtcctcg
     5761 caggtcagtt gccgtggtac ggcccgcttt cgggctgctg cgtttcgaag cgcatcacgt
     5821 cgatggtctt ctgcatctcg gcattggcca gttgctggcg tgccgtcacc gatttggccg
     5881 tggccagttc cgcgtcgata cgcgcttcct cggccagctg gcgcgaagac ttcacgccat
     5941 ccatgccggc atcgcggtag gcggcgatgc tttgcgcctg ggccagtttg tcgctggctt
     6001 ccttcaactc gagttccgat tcatcgccga cgcgcgcgcg ttcggcctgt tgctaggctg
     6061 cctgcgccga ggcaatggcg tcgttgggtg gactgggtgc cgtggcacat gcgctcaata
     6121 ccagcgtggc gcaggcaacg gatgcagcat ggatcgctct catggcagct cctccaggtg
     6181 ggctgcagat ttgagggcgg ggcgctggcg gaagttcgga tggacttgcg ctagagccag
     6241 cgcagctgca cggtgcggcc gacgccgccg gaaagaaaag ccgcctggcc gtattggcgt
     6301 gcgagacgac ggaccaaccc tggttgtgca tcgagcagca gcagcgcggg ctcgggcggc
     6361 cagtcgccgc gcggcg
//
LOCUS       DASZZC010000332         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_541596,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000332 DASZZC010000000
VERSION     DASZZC010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541596"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VHE37_07020"
     CDS             <1..484
                     /locus_tag="VHE37_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35316.1"
                     /translation="NSQGFEGAFNWITPLPGLAFNTAVAYTLALTQVPFNSTGGEVPA
                     GTQMAATPRWQTAVTASYTHYFGGWVSALALTDTYSGKAYNDMNHTHAIYDASLLNVD
                     LSVSRPDWSFAPALAVGINNLTDRRELVGFTAGTSLQTDQTIYNRPRSLSLRLTAEFK
                     "
     gene            489..1856
                     /locus_tag="VHE37_07025"
     CDS             489..1856
                     /locus_tag="VHE37_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT35317.1"
                     /translation="MTTAPPPTETALRRSVLTAFTLPTLVLGIMHGPEGQVQSVYAKH
                     AGVALTALAAAMLFSKMFDAITYPLIGLLSDRSYARSGTRKGWVIGGAAVSLLGIWKL
                     MRPPQEIDAFYFGLWMSVTYLGWKLMEIPLQAWSYVLSEDYAQRSRVQAWRGLAALIG
                     TMLFFGVPYLAVKLGYSDSTEIDFRSLGFSAVVCLIVLPLATLLAVVHVPDGTAAAPP
                     AARRFRLSEVIHAVWNNRPLLHLIAAFLPVSLLGGLMNGVAYLYLDAYLHLSTQFAAI
                     MGLAMLASVVSIPFWSAMAARFERHRVWAVALIVDGLACGAMSLLEPGAAALPLALVI
                     YPVITFTTASTVVVYTMSADIVDHGRLLTGEDHAGLYGAIFVFLQKSVLGVSSALGLA
                     IAGYFGFDATASTQTASGILGIKLPAGILPGLGLIAAAIIIWSYPLTRARVAEIQRQL
                     HKADR"
     gene            1853..2836
                     /locus_tag="VHE37_07030"
     CDS             1853..2836
                     /locus_tag="VHE37_07030"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HVT35318.1"
                     /translation="MSNVKAILQRSLVWDNHACMPLRPEDRNFLPQLERHRAAGATVV
                     ILNVSCDCDAILQPFQMIESFRRWLAERPQEYLVGSSVADIQRAKREGRLAVFFDLEG
                     GIPVQDDPSQVAQLYALGVRWMLLAYNRNNKLGGGCQDEDTGLTAQGRRVLDEMKKCG
                     MVLCCTHTGVRTVREAMEYMQQPVIFSHSNPRAVHDHPRNISDDQIRACAATGGVVNV
                     NGIGIFLGGNDNSTGTYVKHVRYVADLVGPQHVGIGLDYVFDSKELDDFVTGNPHIFP
                     PELGYGAGIRMVEPERIPAIAEALLASGWSDADLGGFLGANNLRVAQAVWK"
BASE COUNT          472 a         1012 c          913 g          516 t
ORIGIN      
        1 caattcgcaa gggttcgaag gggcgttcaa ctggatcacg ccgctccccg gcctggcttt
       61 caacaccgcg gtggcgtaca cgcttgccct gacccaggtg ccgttcaaca gcaccggcgg
      121 cgaagtgcct gccggcacgc agatggcggc cacgccgcgc tggcagaccg cggtcaccgc
      181 cagctacacg cactatttcg gcggctgggt ttccgctctg gcgctcaccg acacctactc
      241 cggcaaggcc tacaacgaca tgaaccacac ccacgccatc tacgacgcca gcctgctgaa
      301 tgtcgatctc tcggtttcgc gccccgactg gtcgttcgcg ccggcgctgg cggtgggcat
      361 caacaacctg accgaccgcc gcgagctcgt cggcttcacc gccggcacct cgctgcagac
      421 cgaccagacc atctacaacc gcccgcgttc gctgagcctg cggctcaccg cggaattcaa
      481 gtagcccgat gaccaccgcc ccgccgccaa ccgaaaccgc gctccggcgc agcgtgctca
      541 cggcattcac cttgccgacg ctggtgctgg gcatcatgca tgggcccgag ggacaggttc
      601 agtcggtcta tgccaagcac gccggcgtcg cgctcaccgc actggccgcg gcgatgctgt
      661 tctcgaagat gttcgacgcc atcacctacc cgctgatcgg cctgctgtcg gaccgctcct
      721 atgcgcgcag tggcacgcgc aagggctggg tgatcggcgg cgccgcggtt tcgctgctcg
      781 gcatctggaa gctgatgcgg ccgccgcagg aaatcgatgc gttttatttc ggcctgtgga
      841 tgtcggtcac ctacctcggc tggaagctga tggagattcc gctgcaggct tggagctatg
      901 tcctgtccga ggactatgcg cagcgctcgc gcgtgcaggc ctggcgcgga ctggccgcgc
      961 tcatcggcac catgctgttc ttcggcgttc cctacctggc ggtgaagctc gggtactccg
     1021 acagcaccga gatcgatttc cgttcgctcg gcttttccgc cgtcgtctgc ctcatcgtgc
     1081 tgccgctggc gaccctgctg gcggtggtcc atgtgccgga tggcaccgct gcagcaccgc
     1141 cggccgcaag gcggttccgc ctgtccgaag tgatccacgc cgtctggaac aaccggccgc
     1201 tgctgcacct gatcgcagcc ttcctgccgg tgagtctgct cggaggattg atgaatggag
     1261 tcgcctacct gtatctggac gcgtacctgc acctgagcac gcagttcgcc gcgatcatgg
     1321 ggctggccat gctcgccagc gtggtcagca tcccgttctg gagcgcgatg gcggcgcgct
     1381 tcgagcgtca ccgcgtgtgg gcggttgcgc tgatcgtcga cgggcttgca tgcggagcga
     1441 tgtcactgct ggagccgggc gccgcagcgc tgccccttgc gctggtgatc tatcccgtca
     1501 tcaccttcac caccgcgagc actgttgtcg tctacaccat gtcggccgat atcgtcgatc
     1561 atgggcgcct gctcaccggc gaggatcacg ccggcctcta cggcgcaatt ttcgttttcc
     1621 tgcagaaatc ggtcctgggc gtgtcgtcgg cgctgggcct ggccatcgcc ggttatttcg
     1681 gcttcgatgc cactgcttcc acgcagacgg cgtcgggcat cctgggcatc aagctccccg
     1741 cgggcatcct gccgggactg ggcctgatcg ccgccgcgat catcatctgg agctatccgc
     1801 tcacgcgcgc acgggtggcg gaaatccagc gtcaactgca caaggcagac cgatgagcaa
     1861 cgtaaaggca attctccagc gcagcctggt atgggacaac cacgcatgca tgccgctgcg
     1921 ccccgaggac cggaacttcc tgccgcagct ggaacgccac cgggcggccg gcgccacggt
     1981 cgtgatcctg aacgtgagct gcgactgcga cgccatcctg cagcccttcc agatgatcga
     2041 gtccttccgc cgctggctgg cggaacggcc acaggaatac ctcgtcggtt cgagcgttgc
     2101 cgacatccag cgcgcgaagc gcgaaggcag gctcgcggtg ttcttcgacc tcgagggcgg
     2161 catcccggtg caggatgatc cgtcccaggt cgcgcagctg tatgcgctgg gcgtgcgctg
     2221 gatgctgctg gcctacaacc gcaacaacaa gctaggcggc ggctgccagg atgaagacac
     2281 cggactcacg gcgcaggggc gccgcgtgct cgacgaaatg aagaagtgcg gcatggtgct
     2341 gtgctgcacc cacaccggcg tccgcaccgt gcgcgaggcc atggagtaca tgcagcagcc
     2401 ggtgatcttc tcgcactcca atccgcgcgc cgtgcacgac catccgcgca acatcagcga
     2461 tgaccagatc cgcgcctgcg ccgcgaccgg cggcgtggtc aacgtcaacg gcatcggcat
     2521 cttcctcggc ggcaacgaca acagcaccgg cacctacgtg aagcacgtgc gctacgtcgc
     2581 cgacctggtc ggtccgcagc acgtcggcat cggcctggac tacgtcttcg acagcaagga
     2641 actggacgac ttcgtcaccg gcaatccgca tatcttcccg ccggagcttg gctacggcgc
     2701 tggcatccgc atggtggaac ccgagcgcat accggccatc gctgaagccc tgctcgcaag
     2761 tggatggagc gatgcggacc tcgggggatt cctcggcgcc aacaacctgc gcgtggcaca
     2821 ggcagtctgg aaatagcatc ggcctagccg ctccagccgc caccctgccc gtcgcgcggc
     2881 ggcggcgcat ggttctgacc ttgcggctgc ggc
//
LOCUS       DASZZC010000333         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_475781,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000333 DASZZC010000000
VERSION     DASZZC010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475781"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1333)
                     /locus_tag="VHE37_07035"
     CDS             complement(23..1333)
                     /locus_tag="VHE37_07035"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT35319.1"
                     /translation="METTETKAAATGAVVAPDTAPRSTLPALPTASWPVRLRGRRKSL
                     AAVAAIALGAACLLRNAVLGSPVPVYVVQSGALTQTVVASGRVTTPARVAVGAEITGR
                     VARIPVAEGQRVQAGALLIQLEDRDQRAAVAQALAARVQAQAHLQSIRDYEFPVARQT
                     LAQALADAQQAREHLERAQQLRAQGYIGQAELDDVRTADDNALARVSAASLQVAAKQP
                     GGSDVVLAQAALAQAQAGLVLAQAALAHTRVHAAAAGTLVAREVEEGDVVQPGHELMA
                     LAVDGDTLIEAQIDEKNLSLLAPGQAARVSADAFAQRRFDAQLVYVNPGVDAARGSVE
                     VKLRVPQPPAYLRQDMTVSVDLEVAQRAHALTVPADALREDGSGSWLLVLERGRAQRR
                     AVRVGLRGQGRIEILDGVSAGEQVISPVAAPALKPGDRARAAGS"
     gene            1466..2305
                     /locus_tag="VHE37_07040"
     CDS             1466..2305
                     /locus_tag="VHE37_07040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HVT35320.1"
                     /translation="MAFDAISQAVAVRPSGFDAFLQMAASQPLLDAQQERAEALRVRQ
                     HDDAEAARRLVLANLRFVISVARGYLGYGLPLADLVQEGSIGLIKAVRRFDPAHGVRL
                     ISYAVHWIRAQIHDYILHNFRLVRHTTTHAQRKLFFNLRKLRHGMQTMSEQEVADMSA
                     QLSVSPQEVRKAEAAFAAGEVALHTQDGEPAPEHWLAAADADPAQIVEQADWNQRMLP
                     RLRRAVAQLDDRSRDIVQRRLLCEGAREELKTLGAKYAVSAERVRQIEARAVSRLREE
                     LAA"
     gene            complement(2536..>2995)
                     /locus_tag="VHE37_07045"
     CDS             complement(2536..>2995)
                     /locus_tag="VHE37_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HVT35321.1"
                     /translation="APSHMNSQPWSFVIVTRPEAVARLGRPATVALRWEMNEGTTRYE
                     ARDAVFGPEFDVFYGAPALIVVCATQPGSLAEMDCAMAAYNIMLAASSMGLGSCWVSH
                     AMPWLARPDGRAALGLSDDVRPVAPVVVGGPAGAPLSPGRYAPQVRVIKE"
BASE COUNT          440 a         1076 c         1053 g          426 t
ORIGIN      
        1 gcagccagct gcgcatgcgc ggctagctgc cggcggcgcg cgcgcggtcg cccggcttga
       61 gcgctggcgc ggccaccggc gaaatcacct gctcgccggc gctgacgcca tcgaggatct
      121 cgatgcgccc ctgcccgcgc aggcccacgc gcaccgcgcg gcgctgcgcg cgcccgcgct
      181 cgagcaccag cagccagctg ccgctgccgt cctcgcgcag cgcatcggcc ggcacggtca
      241 gcgcgtgcgc gcgctgcgcc acttcgagat cgaccgagac ggtcatgtcc tggcgcaggt
      301 aggccggcgg ctgcggcacg cgcagcttca cctcgaccga gccgcgcgcg gcatcgacgc
      361 cgggattgac gtagaccagc tgcgcatcga agcggcgctg cgcaaaggca tccgccgaca
      421 cgcgcgcggc ctggcccggc gccagcaagg aaagattctt ctcgtcgatc tgcgcttcga
      481 tcagcgtgtc gccgtcgact gccagcgcca tcagctcgtg gccgggctgc accacatcgc
      541 cctcctccac ctcgcgcgcc accagcgtgc cggccgcggc ggcgtgcacg cgggtgtgcg
      601 cgagcgcggc ctgcgccagg acgagcccgg cctgcgcctg cgccagtgcg gcctgcgcca
      661 gcaccacgtc gctgccgcct ggctgcttgg ccgccacctg cagcgaggcc gcggagacac
      721 gcgccagcgc gttgtcgtcg gcggtgcgca cgtcgtcgag ctcggcctgg ccgatgtaac
      781 cctgtgcgcg cagctgttgc gcacgctcca gatgctcacg ggcctgctgg gcgtcggcca
      841 gcgcctgcgc cagtgtctgc cgcgccaccg gaaactcgta gtcccggatg ctttgcaagt
      901 gcgcctgcgc ctgcacccgc gccgccagcg cctgtgccac ggcggcgcgc tggtcgcggt
      961 cctcgagttg gatcagcagc gcgccggcct gcacgcgctg gccttcggcc accgggatgc
     1021 gcgccacgcg gccggtgatc tccgcgccca ccgccacgcg cgcgggggta gtcacgcggc
     1081 cgctggccac caccgtctgg gtaagcgcgc cggactggac gacatacacc ggcaccggcg
     1141 atccgagcac cgcgttgcgc agcaggcagg cagcgccgag cgcgatggcc gcgacggcgg
     1201 cgagagactt ccggcgtccg cgcaggcgca caggccagga ggcggtgggc agcgccggca
     1261 gtgtgctgcg cggcgccgta tcgggcgcga ccaccgcgcc cgtggcggcc gctttggttt
     1321 cggtcgtttc caatcgcgcc agtgttcgtc gcgcagtcag cgcaggcatt gatctgcgtc
     1381 agccgcgcgc tggctgatcc aggtcaatga gcaggcgcgc cccgccgcgc aagctgcata
     1441 cacatttttt tatcacggag cggacatggc cttcgacgcg atcagccagg cggtggcggt
     1501 gcggcccagc ggcttcgatg ccttcctgca gatggccgcg tcgcagccgc tgctcgacgc
     1561 gcagcaggaa cgcgccgagg cactgcgcgt gcgccagcac gacgacgccg aggccgcgcg
     1621 ccgcctggtg ctggccaacc tgcgcttcgt catcagcgtg gcgcgcggct atctcggtta
     1681 cggactgccg ctggccgacc tggtgcagga aggcagcatc ggcctgatca aggcggtgcg
     1741 ccgcttcgat ccggcacacg gcgtgcgcct catctcgtac gcggtgcact ggatccgcgc
     1801 gcagatccac gactacatcc tgcacaactt ccgcctggtg cggcacacca ccacacacgc
     1861 gcagcgcaag ctgttcttca acctgcgcaa gctgcggcac ggcatgcaga ccatgagcga
     1921 acaggaagtg gccgacatgt cggcgcagct cagcgtgagc ccgcaggagg tgcgcaaggc
     1981 ggaagccgcg ttcgcggccg gcgaagtggc gctgcatacg caggatggcg agccggcgcc
     2041 cgagcactgg ctggcggcgg cggatgccga tcccgcgcag atcgtggagc aggccgactg
     2101 gaaccagcgc atgctgccgc gcctgcgccg cgcggtggcg cagctcgatg accgctcgcg
     2161 cgacatcgtg cagcggcgcc tgctgtgcga aggcgcgcgc gaggagttga aaacgctggg
     2221 tgcgaaatac gcggtcagcg ccgagcgcgt gaggcagatc gaggcgcgcg cggtgagccg
     2281 gctgcgcgag gagctggcgg cgtgatggcg gtttttttcg ccaccgcacc ggcatttcgc
     2341 gtgttatcaa atattatcta tttttacttt tatacaattt tttataacac gcgaaatgcc
     2401 cggcggtggg tcagtttgat cttgtcgtcc cgagcgcagc gagggatctc gccggcgtgg
     2461 atgagattcc tcgctacgcc cggaatgaca acatgacccg ttgccggatg ccggtgcgac
     2521 gaggctggaa aacgcctatt ccttgatcac gcgcacctgc ggcgcgtagc ggccgggcga
     2581 cagcggcgcg ccggcgggac cgccgaccac gaccggcgcg acggggcgca catcgtcgga
     2641 caggccgagc gcggcacggc catcagggcg cgccagccac ggcatggcat ggctgaccca
     2701 gcacgagccc aggcccatgc tgctggccgc cagcatgatg ttgtacgcgg ccatggcgca
     2761 gtccatctcc gccaggctgc caggctgcgt ggcgcacacc acgatcagcg cgggcgcgcc
     2821 gtagaacacg tcgaactcgg ggccgaagac ggcgtcgcgc gcctcgtagc gcgtggtgcc
     2881 ctcgttcatc tcccagcgca gcgccaccgt ggccgggcgc ccgaggcgcg ccaccgcctc
     2941 gggcctggtc acgatgacga acgaccacgg ctgcgagttc atgtggctgg gcgcc
//
LOCUS       DASZZC010000334         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_30703,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000334 DASZZC010000000
VERSION     DASZZC010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30703"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VHE37_07050"
     CDS             complement(<1..887)
                     /locus_tag="VHE37_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT35322.1"
                     /translation="MNTDFHPSWLIRHHQVQSILATKGPRRRAWLKRGSRLEQLQQHH
                     VLDAGSGVRLTGMHSPQPAGTAPRGLCVLIHGWEGSHESVYLYSMACALHLAGYSVFR
                     LNLRDHGASHHLNEELFHSARMDEVLGTIARVLEFDAARPLFVIGFSLGGNFALRVAL
                     QGPARGIAPALTIGISPAINPGATLAAIDSGPFLFRRYFLDKWRKTLRAKKAAWPHYD
                     FAAFDDLENFVETTRYFVETHTEYASLGDYLAQYTLTPAMLMNSPAPLAVITARDDSV
                     IPFRDFDGLREGGPVARFLS"
     gene            complement(907..>1742)
                     /gene="dapE"
                     /locus_tag="VHE37_07055"
     CDS             complement(907..>1742)
                     /gene="dapE"
                     /locus_tag="VHE37_07055"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HVT35323.1"
                     /translation="DMKSGLAAMVCAAERFAATKKNFRGTIAFLVTSDEEGPCRHGTR
                     HVVEVLKQRGVKPDYAVVGEATGDRQLGDRIMIGRRGSLGCDLKVLGKQGHVAYPGKA
                     DNPIHRVLPALAQLAATTWDDGNAHFPPTSFQVSNFKSGTGANNVIPGDAEIIFNFRY
                     NTEHSAESLKARVHAVLDKHGVRYEPKWWNTGEPFLTQQGALIAAAQSAIGQVAGLQP
                     LLFTGGGTSDARFIAPWGAQTVEIGPVNDSIHKLNEHVLVSDLDRLSSIYEKILEQLL
                     N"
BASE COUNT          278 a          550 c          611 g          303 t
ORIGIN      
        1 gagaggaagc gcgccaccgg cccgccctcg cgcagcccgt cgaagtcgcg gaaaggaatc
       61 accgaatcgt cgcgcgcggt gatcaccgcc agcggcgccg gcgagttcat cagcatcgcc
      121 ggcgtcagcg tgtactgcgc caggtaatcg ccgaggctgg cgtactcggt gtgcgtctcg
      181 acgaagtagc gcgtggtctc gacgaaattt tccaggtcat cgaacgcggc gaagtcgtag
      241 tgcggccagg ccgccttctt cgcgcgcagc gtcttgcgcc acttgtcgag gaagtagcgg
      301 cgaaagagga aagggccgga atcgatcgcc gccagtgtgg cgccggggtt gatcgcgggc
      361 gagatgccaa tggtgagtgc gggcgcgatg ccgcgcgccg ggccctgcag cgcgacgcgc
      421 agggcgaagt tgccgccgag cgagaagccg atgacgaaca gtggccgcgc cgcgtcgaac
      481 tcgagcacgc gcgcgatggt gccgagcacc tcgtccatgc gcgcggagtg gaacagttct
      541 tcgttgaggt gatggctggc gccgtggtcg cgcaggttca ggcggaacac gctgtaaccg
      601 gcgaggtgca gcgcgcaggc catggagtag agatagaccg attcgtgcga gccctcccag
      661 ccgtggatca gcacgcacag gccgcgcggc gccgtgcccg caggttgcgg cgagtgcatg
      721 ccggtcaggc gcacgccgct gccggcgtcg agcacgtgat gctgctgcag ttgttccagc
      781 cggctgccgc gcttcagcca cgcgcgccgg cgcgggccct tggtggcaag gatcgattga
      841 acctggtggt gccggatcag ccagctggga tggaaatcgg tgttcatgtc ttacgccgtc
      901 attccatcaa ttcagcagct gctccaggat tttttcgtag atcgacgaaa ggcggtcaag
      961 gtcgctcacc aatacgtgct cgttgagctt gtggatgctg tcgttgaccg ggccgatctc
     1021 cactgtctgc gcgccccagg gcgcgatgaa acgcgcatcg ctggtgccgc cgccggtgaa
     1081 cagcagcggc tgcagcccgg caacctggcc gatcgcagac tgcgccgccg caatcagcgc
     1141 gccctgctgg gtcaggaacg gctcaccggt gttccaccac ttcggctcgt agcgcacgcc
     1201 gtgcttgtcg agcaccgcgt gcacgcgcgc cttcagcgac tccgcgctgt gctcggtgtt
     1261 gtagcggaag ttgaagatga tctcggcgtc gccggggatc acgttattgg cgccggtgcc
     1321 ggacttgaaa ttcgacacct ggaacgaggt cggcggaaag tgcgcgttgc cgtcgtccca
     1381 cgtcgtcgcc gctagttgcg ccagcgccgg cagcacgcgg tggatcgggt tgtcggcctt
     1441 gccgggatac gcgacgtggc cctgcttgcc gagcactttt agatcgcagc ccagcgaacc
     1501 gcggcggccg atcatgatgc ggtcgccgag ttgccggtcg ccggtggctt cgccgaccac
     1561 cgcgtaatcg ggtttcacgc cgcgctgttt cagcacttcc accacgtgcc gggtgccgtg
     1621 gcggcagggg ccttcctcgt cgctggtgac gaggaaggcg atggtgccgc ggaaattctt
     1681 cttcgtcgcg gcaaagcgct cggccgcgca gaccatggcg gcgagtcccg atttcatatc
     1741 cg
//
LOCUS       DASZZC010000335         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_604489,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000335 DASZZC010000000
VERSION     DASZZC010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604489"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VHE37_07060"
     CDS             <1..636
                     /locus_tag="VHE37_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HVT35324.1"
                     /translation="VLCEPYETLVADVEEANQGGVIQGMAERGGKMVNMVPDGKGRVR
                     LEYTIPSRGLIGFHTEFMTMTSGTGLLFHNFDHFGPKSEMSGIGQRHNGVMIANEQGM
                     CLAYALYNLQDRGRLMVDHGDNVYEGQIVGIHSRDNDLVVNPLKGKKLTNMRSAGADE
                     KLILTPPVKFSLEQALEFINDDELVELTPASIRVRKKFLKEHERKRADRAD"
     gene            655..810
                     /locus_tag="VHE37_07065"
                     /pseudo
     CDS             655..810
                     /locus_tag="VHE37_07065"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(913..2235)
                     /locus_tag="VHE37_07070"
     CDS             complement(913..2235)
                     /locus_tag="VHE37_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35325.1"
                     /translation="MYLRLFGWFLLANLLTLALSVFVANRIAQREWHAYSDWPAMARS
                     AVAHYASGGRRGLWEWAEPLRHSGIIIDLLDAHGESVLRRHSRLPWTEEEPRLLGETE
                     VELHPRPDLTLASVPVQGPHEESWHFIAFRSPPPRGPATVFEHVATEIIVSLLVVGVV
                     GWWVTRTIARPVSALQHAARRMAQGDLSARAGAQAAREPGELGELARDFDHMAARIET
                     LVQGNRSLLQDISHELRSPLARLQIALGLARRDAGAGEAHFDRAEREVSRLDHLIGEV
                     LTLSRMEIDLPGMQTQRVEFSALLRELVEDNGAGEGSVPQVVLAAPQPVHVQGDPELL
                     ERAAGNLISNALKYGKADGRVELTLTRSADKAVLTVADDGPGVPADELPRLFEPFFRG
                     SNAARASGSGLGLSIVDRIVRAHGGTVSAENRAGGGLVVTLTLPALES"
     gene            complement(2253..2939)
                     /locus_tag="VHE37_07075"
     CDS             complement(2253..2939)
                     /locus_tag="VHE37_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT35326.1"
                     /translation="MQRILIADDDRELCSLLADYLGHEGFEVHCVHDANAALDALVDE
                     RRRPDLLILDVMMPGKDGLQALRELRQQHRLPVIMLSARGEPIDRVVGLELGADDYLS
                     KPCLPRELLARVHARLRRPANGVAEALQIGDLKLDPGERRAWAAGRELQLTGAEYSLL
                     LALAQAAGQVVDKATLTRLALGRPIERFDRAIDVHVSRLRRKLAEAGASGPGIESVRG
                     AGYLLTGHAA"
     gene            3072..3494
                     /locus_tag="VHE37_07080"
     CDS             3072..3494
                     /locus_tag="VHE37_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVT35327.1"
                     /translation="MHKMFKTLLIPAAIAVSLAGGAALAQPFGGGHHQMMVAALDQLD
                     LSDSQWLQIHKIMQAQREERHAQDHHALMAAFESLNPDSPDYAAQVQAAQDQAAAAAR
                     EHVAHAADVKKQIYAVLTPEQKSKLATLKAAHQHGAGG"
     gene            complement(3568..4812)
                     /locus_tag="VHE37_07085"
     CDS             complement(3568..4812)
                     /locus_tag="VHE37_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HVT35328.1"
                     /translation="MKLLKQAMVASLSVSAVVLAACGGGGSSTTSDNLTLASATLELG
                     QADFTGGNPNQGTTVSEKALNNPQGNVATNGTLFYVVDTNNSRVLGYNAVPTVNNTGA
                     NFALGQPDLTSGSPNNNGGTANLSKVMAFPSSVAISGTKLVVTDTDNNRVLIWNTLPT
                     GNVAPDVVVGQAGLNGSASGTTSSTLSSPQAAMIANGKLVVADTGNNRVLIWNTVPTT
                     NGVAADVVLGQADFVSNVSVLTPTQASLRNPQGVWTDGFNLLVADSGTNRVLYWKQIP
                     TTNNANALYVMGQPSFIASAGSSGSALMKGPTGVFYDGTRIFVADTLNNRVLVFNGFP
                     LASGTVASAVLGQEDFSHSQFNDDNGAAVDPQDGVNEVKPTQRTLFQPTGVMSTGTNV
                     YVSDTRNNRIMVYAPPYNAPGS"
     gene            complement(5026..>5428)
                     /locus_tag="VHE37_07090"
     CDS             complement(5026..>5428)
                     /locus_tag="VHE37_07090"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVT35329.1"
                     /translation="PAAAPAPATAAAAATEIVAGAKPPVAPAPIRLTQAPGAGSPMRP
                     YAAAPSAAPAAAPSAGGNWTVVVLSVRSIGSARDEVARLEARGLAAETHAHGTMNRVT
                     VGRFTSKESAHAYAMELASREHLQAWVTQVD"
BASE COUNT          864 a         1816 c         1881 g          867 t
ORIGIN      
        1 gtgctgtgcg agccgtacga gacgctggtg gccgacgtcg aggaagcaaa ccagggcggc
       61 gtgatccagg gcatggccga gcgcggcggc aagatggtca acatggtccc cgacggcaag
      121 ggccgcgtgc gcctggaata caccattccg tcgcgcggcc tgatcggctt ccacaccgag
      181 ttcatgacca tgacctcggg tacgggattg ctgttccaca acttcgatca tttcggaccg
      241 aagtcggaga tgagcggcat cggccagcgc cacaacggcg tgatgatcgc caacgagcag
      301 ggcatgtgcc tggcttacgc gctgtacaac ctgcaggacc gcggccgcct gatggtggac
      361 cacggcgaca acgtctacga aggccagatc gtgggcattc actcgcgcga caacgacctg
      421 gtggtcaacc cgctcaaggg caagaagctg accaacatgc gctcggccgg cgccgatgaa
      481 aaactcatcc tgacgccgcc ggtgaagttc tcgctggagc aggcgctgga gttcatcaac
      541 gacgacgagt tggtggagct gacgccggcg tcgatccgcg tgcgcaagaa gttccttaag
      601 gaacacgagc gcaagcgcgc ggatcgcgcc gactgatcca gccagaaagc tgatcttccg
      661 cccccggcga agttctcgct ggagcaggcg ctggagttca tcgtcactca agcgagcgac
      721 gagctggtgg agctgacgcc ggcgtcgatc cgcgtgcgca agaagttcct caaggagcac
      781 gagcgcaagc gcgcggatcg cgccgattga gccttaccgc actggcatct gcgatgttat
      841 aaattattat tcaaataata atttgtataa ttttttataa cacgcaaaat gcccgtgcgg
      901 taagcggatt tttcagctct caagcgccgg cagcgtaagc gtcaccacca atccgccacc
      961 cgcgcggttc tccgccgaca ccgttccgcc gtgcgcgcgc acgatgcggt cgacgatcga
     1021 caggcccagt cccgagccgc tggcgcgcgc ggcgttgctg ccgcggaaga acggttcgaa
     1081 caggcgtggc aattcgtcgg ccggcacgcc gggaccgtcg tcggccacgg tcagcacggc
     1141 cttgtcggcg ctgcgcgtca gcgtgagctc gacgcgaccg tcggccttgc cgtacttcag
     1201 cgcattgctg atcaggttgc ccgcggcgcg ttccagcagt tcggggtcgc cctgcacgtg
     1261 gaccggctgt ggcgcggcga gcaccacctg cggcacgctg ccctctcccg cgccgttgtc
     1321 ttcgaccaat tcgcgcagca gcgcggaaaa ttccacgcgc tgcgtctgca tgccgggcag
     1381 gtcgatctcc atgcggctga gcgtgagcac ttcgccgatc aggtgatcga gccgcgacac
     1441 ctcgcgctcg gcgcggtcga agtgcgcctc gccggcgccg gcatcgcgcc gcgccagtcc
     1501 cagcgcgatc tgcagccgcg ccagcggtga gcgcagctcg tgcgagatgt cctgcagcag
     1561 gctgcggttg ccctgcacca gcgtctcgat gcgcgcagcc atgtggtcga agtcgcgcgc
     1621 cagctcgccc agttcccccg gctcccgcgc cgcctgcgcg ccggcgcgcg ccgacaggtc
     1681 gccctgcgcc atgcgccgcg ccgcgtgctg cagcgccgac accgggcgcg cgatggtgcg
     1741 cgtgacccac cagccgacca cgcccaccac cagcagcgag acgatgatct cggtggccac
     1801 atgttcgaac acggtggcgg ggccgcgcgg cggcggcgag cggaaggcga tgaagtgcca
     1861 gctttcctcg tgcggcccct gcaccggcac gctggccagg gtgagatcgg ggcgcgggtg
     1921 caattccact tcggtctcgc cgagcaggcg cggctcttcc tcggtccacg gcaggcgcga
     1981 gtggcgccgc agcacgcttt cgccgtgcgc atcgagcagg tcgatgatga tgccggaatg
     2041 gcgcagcggc tcggcccatt cccacaggcc gcggcggccg ccgctggcgt agtgcgccac
     2101 ggcgctgcgc gccatcgccg gccagtcgga ataggcatgc cactcgcgct gcgcgatgcg
     2161 gttggccacg aacaccgaca gggcgagcgt gagcagattg gccagcagga accagccgaa
     2221 cagccgcagg tacaaccggc tgacgcgcgg gttcaggcag cgtgaccggt gagcaggtag
     2281 ccggcgccgc gcaccgattc gatcccgggc ccgctggcgc cggcctcggc cagcttgcgc
     2341 cgcaggcggc tgacgtggac gtcgatggcg cggtcgaagc gttcgatcgg ccggcccagc
     2401 gccagccggg tcagcgtggc cttgtccacc acctgcccgg cggcctgcgc cagtgccagc
     2461 agcagcgagt actcggcgcc ggtgagctgc agctcgcggc cggccgccca ggcgcggcgc
     2521 tcgcccgggt cgagcttgag atcgccgatc tgcagcgcct cggccacgcc gttggccggc
     2581 cggcgcagcc gcgcgtgcac gcgcgccagc agctcgcgcg gcaggcaggg cttggacagg
     2641 taatcgtcgg cgccgagctc gagtccgacc acgcggtcga tcggctcgcc gcgcgcggac
     2701 agcatgatca ccggcagccg gtgctgctgg cgcagttcgc gcagcgcctg caggccgtcc
     2761 ttgcccggca tcatcacgtc gaggatcagc aggtcgggcc gcctgcgctc gtcgaccagc
     2821 gcgtcgagcg ccgcgttggc gtcgtgcacg caatgcacct cgaagccctc gtgaccgagg
     2881 tagtcggcca gcagcgagca cagctcgcgg tcgtcgtcgg cgatcaggat tctctgcatg
     2941 cgcatacttt ggtgtcgcgc tttacattcg gcaacgaggc cggccggcca tttacctaac
     3001 tttacggcgg cgcaaccctg ctttaccgcg gcggccccta taaaagtcgc gcatccccaa
     3061 cggagtgttc catgcacaag atgttcaaga ccctgctgat cccggcggcg atcgccgtct
     3121 cgctggccgg cggcgcggcg ctggcccagc cgttcggcgg cggccatcac cagatgatgg
     3181 tggcggcgct cgatcagctc gacctgtccg acagccagtg gctgcagatc cacaagatca
     3241 tgcaggcgca gcgcgaagag cgccatgcgc aggaccacca cgcgctgatg gcggcgttcg
     3301 agtcgctcaa ccccgacagc cccgactacg ccgcgcaggt gcaggcggcg caggaccagg
     3361 cggcggcagc agcgcgcgag cacgtggcgc atgcggccga cgtcaagaag cagatctacg
     3421 cggtgctgac gccggaacag aagagcaagc tggccacgct gaaggccgcg caccagcacg
     3481 gcgccggcgg ataagcacgg aaagaatccg caccaacaaa aaaaccgccg gcagtgctgc
     3541 cggcggtttt ttcacatccg ggaaatatca gctgcccggc gcgttgtacg gcggcgcgta
     3601 gaccatgatg cggttgttgc gcgtgtcgga cacgtagaca ttggtgccgg tggacatgac
     3661 gccggtgggc tggaacaacg tgcgctgcgt cggcttgacc tcgttgacgc cgtcctgcgg
     3721 atcgaccgct gcgccgttgt cgtcattgaa ctggctgtgc gagaaatctt cctggccgag
     3781 cactgccgag gctaccgtgc cgctggccag cgggaagccg ttgaatacca gcacccggtt
     3841 gttgagcgta tcggcgacga agatgcgcgt gccgtcgtag aacacgccgg tagggccctt
     3901 catcagcgcg ctaccggacg acccggcgct ggcaatgaac gagggctggc ccatcacgta
     3961 cagcgcgttg gcgttgttgg tggtgggaat ctgcttccag tacagcacgc ggttggtgcc
     4021 gctgtcggcc accagcaggt tgaagccgtc ggtccacacg ccctgcggat tgcgcaggct
     4081 ggcctgggtc ggcgtcagca ccgagacgtt gctgacgaag tcggcctggc ccagcaccac
     4141 gtcggccgcg acgccgttgg tggtcggcac cgtgttccag atcagcacgc ggttgttgcc
     4201 ggtgtcggcc accaccagct tgccgttggc gatcatcgcc gcctgcggcg acgacagcgt
     4261 ggaggaagtg gtgccggatg ccgagccgtt gaggccagcc tggccgacca ccacgtcggg
     4321 cgccacgttg ccggtcggca gcgtgttcca gatcagcact cggttgttgt cggtgtcggt
     4381 gaccaccagc ttggtgccgg agatggccac gctgctgggg aaggccatca ccttggacag
     4441 gttggcggta ccgccgttgt tgttcggcga gccgctggtg aggtccggct ggccgagcgc
     4501 gaagttggcg ccggtgttgt tcaccgtcgg caccgcgttg tagccgagca cgcggctgtt
     4561 gttggtgtcg accacgtaga acagcgtgcc gttggtggcc acgttgccct gcgggttgtt
     4621 cagggccttc tcggaaacgg tggtgccctg gttgggattg ccgccggtga agtcggcctg
     4681 gcccagttcc agcgtcgccg aggcgagggt gagattgtcg ctggtggtgc tggacccgcc
     4741 gccgccgcag gccgcgagca cgacagccga gaccgacaac gacgccacca tggcttgctt
     4801 gagcagcttc atcgaaacga ttcctgaggt ttgaaaattg agtttagctt tgcgtcttgt
     4861 cgggcggccg gtggccaccg cgttaagttg tgcaaacccg ttgccgcccg cggccagcgc
     4921 gtctgcggcg cgcaattgta ctgaattccg tgccggcggg aggccggcct cagaccgcgg
     4981 gtttgccggg tggttccgcg ggcgggcgcg ccatgcgcgg cccgctcaat cgacctgcgt
     5041 gacccaggcc tgcagatgct cgcgcgaggc cagttccatg gcgtaggcgt gggccgattc
     5101 cttgctggtg aagcggccga cggtgacacg gttcatcgtg ccatgcgcat gcgtctcggc
     5161 ggccaggccg cgcgcctcca gccgggccac ctcgtcgcgt gcggagccga tgctgcgtac
     5221 cgacagcacc accacggtcc agttgccgcc ggcagacggc gctgcggcgg gcgctgccga
     5281 cggtgccgcc gcgtacggcc gcatgggcga gccggcgccc ggcgcctgcg tcaggcggat
     5341 cggtgccggc gccaccggcg gcttcgcccc tgctacgatt tccgtagcag cggcggccgc
     5401 ggttgccggc gccggcgcag cggccggg
//
LOCUS       DASZZC010000336         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_930636,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000336 DASZZC010000000
VERSION     DASZZC010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930636"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VHE37_07095"
     CDS             complement(<1..682)
                     /locus_tag="VHE37_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVT35330.1"
                     /translation="MKSVAPVPAELYVATIEQPASELLATPRGGTHPLENELRRLTVA
                     VLEERLQKMLRRADETLIDMSERAESPAQQHAHFDAMRILELEKATLLRRFRQEIERA
                     FDALQAPASRTAAGHVPLLPDQELEENVQASRMAARSKMRHKEQLAEVEQRLDMLVRD
                     LGLQIGNRPLSPARICQAWRSAMDPLDLAFNVRRVFMQLFERLVMDDLGELYQAALST
                     MQRMGSLQQ"
     gene            884..1909
                     /locus_tag="VHE37_07100"
     CDS             884..1909
                     /locus_tag="VHE37_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HVT35331.1"
                     /translation="MKLHSSYAQLDAALRRRILVLDGAMGTMIQRHKLEEADYRGARF
                     KDWPRDVKGNNDLLVLTQPEIIRGIHREYLEAGSDLVETNTFNAQVISMADYGMEALS
                     YELNAEAAKLARAECDAMTKNTPQQPRFVCGALGPTNRTASISPDVNDPGKRNVTYDE
                     LVAAYHEQARGLVDGGADVLLIETIFDTLNAKAAIFACETLFDERDMRWPVIISGTIT
                     DASGRTLSGQVTEAFWNSVRHARPLAVGLNCALGAKDMRAYIAELARVADCYVSCYPN
                     AGLPNAFGEYDETPHDMACVIEEFASAGLVNIVGGCCGTTPPHIAHIREHVARKSPRF
                     LPTAKAA"
     gene            1912..4662
                     /gene="metH"
                     /locus_tag="VHE37_07105"
     CDS             1912..4662
                     /gene="metH"
                     /locus_tag="VHE37_07105"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HVT35332.1"
                     /translation="MTLRLSGLEPLNIGPGSLFVNVGERTNVTGSAKFRDLIKAGDYS
                     GALTVARQQVEAGAQVIDVNMDEGMLDGKAAMERFLKLIAGEPDICRVPVMIDSSKWE
                     VIEAGLKCVQGKPIVNSISMKEGEEKFIREARLCRKYGAAVVVMAFDEQGQADSLERR
                     KEICARAYKILTEKIGFPAEDIIFDPNIFAVATGIAEHNNYGVDFIEATRWIKQNLPG
                     ALVSGGVSNVSFSFRGNNPVREAIHSVFLYHAIKAGMDMGIVNAGALVVYDTLDPELR
                     ERIEDVILNRRPDGTERLVEIAGKFKGEAQQAKGEDLSWRSLPTGERITHALVKGIDQ
                     YVEADTEELRAQISARGGRPIEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKK
                     AVAYLIPYIEAEKKPGDAAQAKGKIIMATVKGDVHDIGKNIVGVVLQCNNYDVVDLGV
                     MVPAQKILDAAKEHKADVIGLSGLITPSLDEMVNFAREMKRQGYTLPLLIGGATTSRA
                     HTAVKVAPQYEGPVVWVKDASRSVPVVAALLSDEQRPKLMAEVQKDYAAIRERYANRE
                     KEKFVTLAAARANRTPIEWKDYHPPRPHMLMQQAHDISAKEARGHATQFVRVFRDYPL
                     AELREYIDWQPFFISWEMKGKYPDLLNNPATSEAARKLYADAQAMLDRIIAEKWLVAR
                     GVIGLFPANAIDDDIELYTDESRSQQLAKLHQLRQQSEHREGVPHRSLADYVAPKATA
                     LPDYVGAFAVSAGFGCAEKAAEFKKNHDDYNAILLESVADRLAEAMAERLHQRVRKEF
                     WGHVPDEHLSNDELIAEKYAGIRPAPGYPACPEHTEKATLWQLLEVEKNIGMKLTESM
                     AMWPAAAVSGWYFSHPQAQYFVVGRIGKDQVADYAARKGWSLREGEKWLAPNLGYEPE
                     DS"
BASE COUNT          925 a         1524 c         1559 g          756 t
ORIGIN      
        1 cctgctgcag gctgcccatg cgctgcatgg tggacagcgc ggcctggtac agctcgccca
       61 ggtcgtccat caccagccgt tcaaacagct gcatgaacac gcggcgcacg ttgaaagcaa
      121 gatcgagcgg gtccatcgcg ctgcgccagg cctggcagat gcgcgctggt gacagcgggc
      181 ggttgccgat ctgcaggccg agatcgcgca ccagcatgtc cagtcgctgc tcgacttcgg
      241 ccagttgttc cttgtggcgc atcttgctgc gcgcggccat gcgcgaggcc tgcacgtttt
      301 cctcgagttc ctgatcaggc agcagcggca catggccggc tgcggtgcgc gaggcaggcg
      361 cctgcaacgc atcgaaagca cgttcgattt cctggcggaa gcggcgcagc agagtggcct
      421 tttccagttc gaggatgcgc atggcatcga aatgcgcgtg ctgctgggcg ggcgattcgg
      481 cgcgctcact catgtcgatc agcgtttcgt cggcgcggcg cagcatcttc tgcagccgct
      541 cctccagcac cgccacggtg aggcggcgca gttcgttttc cagcggatgc gttccgccgc
      601 gcggcgtggc cagcaattcg gaggccggct gctcgatggt ggcgacgtag agttctgcgg
      661 gaacgggcgc gacggatttc acgctgcaca atctaggtat tggcggcgcc cgacgcacta
      721 gaaacaacca ccagatgaac cttgcgtcag ttgtcgggcg ggcggatttg cggcgtcgct
      781 gcgccgacag acgggggtac cccgggctgg cctgtcgcag cgcttcaaca tcatccgctt
      841 atccgtatat caggatataa tcggctcctt gcggagaatg gccatgaaac tgcattccag
      901 ctacgcccaa ctcgacgccg ccctgcggcg gcgcatcctg gtgctcgacg gcgccatggg
      961 caccatgatc cagcgccaca agctcgagga agccgactat cgcggcgcac gcttcaagga
     1021 ctggccgcgc gacgtcaagg gcaacaacga cctgctggtg ctgacgcagc cggagatcat
     1081 tcgcggcatc caccgcgaat acctggaagc cggctccgac ctggtcgaga ccaatacctt
     1141 caatgcccag gtcatttcca tggccgacta cggcatggaa gcgttgtcgt acgaactgaa
     1201 cgcggaagcg gcgaagctcg cacgcgcgga atgcgacgcc atgacgaaga acacgccgca
     1261 gcagccgcgc ttcgtctgcg gcgcgctggg tccgaccaac cgcacggcga gcatcagccc
     1321 tgacgtgaac gatccgggca agcgcaatgt cacctacgac gagctggtcg ctgcgtatca
     1381 cgagcaggcg cgcgggctgg tcgacggggg cgccgacgtg ctgttgatcg aaacgatttt
     1441 cgacacgctc aatgccaagg ctgcgatctt cgcgtgcgag accttgttcg acgagcgcga
     1501 catgcgctgg ccggtgatca tctccggcac gatcacggac gcctccgggc gcaccctgtc
     1561 cggccaggtg acggaggcat tctggaattc cgtgcggcat gcgcgaccgc tcgcggttgg
     1621 cctcaactgt gcgctgggcg ccaaggacat gcgcgcgtat atcgccgaac tggctcgcgt
     1681 ggccgactgc tacgtgtcgt gctatcccaa cgcgggcctg ccaaacgcct tcggcgaata
     1741 cgacgagacg ccgcacgaca tggcctgcgt gatcgaggaa ttcgcctccg ccggcctggt
     1801 caacatcgtc ggcggctgct gcggcaccac gccgccacac atcgcgcata tccgcgagca
     1861 cgtggcgcgc aagtcgccgc gtttcctgcc cactgccaag gcagcctgac gatgacgctt
     1921 cgattgtccg gcctggagcc gctcaacatc ggcccgggct cgttgttcgt caatgtcggc
     1981 gagcgcacca acgtgaccgg ctcggccaag ttccgcgatc tgatcaaggc cggcgactac
     2041 agcggcgcgc tcaccgtcgc gcgccagcag gtcgaggcgg gcgcgcaggt gatcgacgtc
     2101 aacatggacg agggcatgct cgacggcaag gccgccatgg agcgtttcct caagctcatc
     2161 gccggcgagc cggacatctg ccgcgtgccg gtgatgatcg actcctccaa gtgggaagtg
     2221 atcgaggccg gcctcaagtg cgtgcagggc aagcccatcg tcaactcgat ttccatgaag
     2281 gaaggcgagg agaaattcat ccgcgaggcg aggctgtgcc gcaagtacgg tgccgccgtg
     2341 gtggtgatgg cgtttgacga gcagggccag gcggacagtc ttgaacggcg caaggaaatc
     2401 tgcgcgcgcg catacaagat cctgacggag aaaatcggct tcccggccga ggacatcatc
     2461 ttcgacccga acatcttcgc ggtcgccacc ggcattgccg agcacaacaa ctacggcgtg
     2521 gatttcatcg aggccacgcg ctggatcaaa caaaatcttc ccggcgcgct ggtctccggc
     2581 ggcgtgtcga acgtgtcgtt ctcgttccgc ggcaataatc ccgtgcgcga agcgatccac
     2641 agcgtgttcc tgtaccacgc catcaaggcc ggcatggaca tgggcatcgt caacgccggc
     2701 gcgctggtgg tatacgacac gctggacccc gaactgcgcg aacgcatcga ggacgtgatc
     2761 ctcaaccggc gtccggacgg caccgaacgg ctggtcgaga tcgccggcaa gttcaagggc
     2821 gaagcgcagc aggccaaggg tgaggacctg tcctggcgca gcctgccaac cggtgagcgc
     2881 atcacgcacg cgctggtcaa gggcatcgac caatatgtcg aagccgacac cgaggaactg
     2941 cgcgcgcaga tttcggcgcg cggcggccgg ccgatcgaag tcatcgaagg cccgctgatg
     3001 gacggcatga acgtggtcgg cgatttgttc ggcgccggca agatgttcct gccgcaggtg
     3061 gtgaaatcgg cgcgcgtgat gaagaaggcg gtggcctacc tgatcccgta catcgaagcg
     3121 gaaaagaagc ccggcgacgc cgcgcaggcc aagggcaaga tcatcatggc cacggtcaag
     3181 ggcgacgtgc acgacatcgg caagaacatc gtgggtgtcg tgctccagtg caacaactac
     3241 gacgtggtcg acctcggcgt gatggtgccg gcgcagaaga tcctcgacgc ggcgaaggag
     3301 cacaaggccg atgtcatcgg cctgtccggc ctgatcacgc cgtcgctcga cgagatggtg
     3361 aacttcgcgc gcgaaatgaa gcgtcagggc tacacgctgc cgttgctgat cggcggcgcc
     3421 accacttcgc gcgcgcacac ggcggtgaaa gtcgcgccgc agtacgaagg cccggtggtc
     3481 tgggtgaagg acgcctcgcg ctcggtgccg gtggtggccg cgctgctgtc ggacgagcag
     3541 cggccgaagc tgatggccga ggtgcagaag gattacgccg ccatccgcga gcgctacgcc
     3601 aatcgcgaga aggaaaagtt cgtgaccctg gccgctgcac gcgccaaccg cacgccgatc
     3661 gagtggaagg actaccaccc gccgcgcccg cacatgctga tgcagcaggc gcatgacatc
     3721 agcgccaagg aagcgcgcgg gcacgcgacc cagttcgtgc gcgtgttccg cgactacccg
     3781 ctggccgagc tgcgcgaata catcgactgg cagccgttct tcatttcgtg ggagatgaag
     3841 ggcaagtatc ccgacctgct caacaatccg gcgaccagcg aggcggcgcg caagctgtac
     3901 gccgacgcac aggccatgct cgaccgcatc atcgccgaga aatggctggt cgcacgcggc
     3961 gtcatcggcc tgtttccggc caacgcgatt gacgatgaca tcgagcttta caccgatgaa
     4021 tcgcgctcac agcagctggc gaaactgcac cagctgcgcc agcagagcga acaccgcgaa
     4081 ggagttcctc atcgcagcct ggccgactac gtcgcgccga aagcaaccgc gctgccggac
     4141 tacgtgggcg ccttcgccgt gagcgcaggt tttggctgcg ccgagaaggc agcggagttc
     4201 aagaagaacc acgacgatta caacgccatc ctgctggaat cggtggccga tcgccttgcg
     4261 gaggctatgg ccgaacgcct gcaccagcgc gtgcgcaagg aattctgggg ccacgttcca
     4321 gacgagcacc tgtccaacga cgaactgatt gccgagaagt acgcgggcat ccgcccggcg
     4381 cccggctacc cggcgtgccc cgagcacacc gagaaggcca cgctgtggca gttgctcgaa
     4441 gttgagaaga acatcggtat gaagctgacc gagagcatgg ccatgtggcc ggccgccgcc
     4501 gtcagcggct ggtacttctc gcacccgcag gcgcagtact tcgtggtcgg ccgcatcggc
     4561 aaggaccagg tggccgacta cgcggcacgc aagggctgga gcctgcgcga aggggagaaa
     4621 tggctggcgc ccaatctggg ctacgaaccc gaggacagct gacgcgcttg cgccacatcg
     4681 ccgccgacgg cactatcttg cgcgcgccgt gcagcggcgt tccgacaatt aaaaagggaa
     4741 tccacgagtg gccaagccga agat
//
LOCUS       DASZZC010000337         4596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_542406,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000337 DASZZC010000000
VERSION     DASZZC010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542406"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VHE37_07110"
     CDS             <1..379
                     /locus_tag="VHE37_07110"
                     /inference="COORDINATES: protein motif:HMM:NF036901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endoglucanase"
                     /protein_id="HVT35333.1"
                     /translation="NRNDLRCGDGWNQEDLSIFSRDQQDAPDDDGGRAVEGFCRPYVQ
                     RAQGVIERVHWSEHERVFSAALQLDPAIPGETEVFLPRRLGNAFELRVTGTADCRHVP
                     GEQRVYLSARSAGNVAIEIRCKG"
     gene            complement(380..712)
                     /locus_tag="VHE37_07115"
     CDS             complement(380..712)
                     /locus_tag="VHE37_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35334.1"
                     /translation="MKPLSPLLAVLLLAACAADDHTPAGACHAEAGRRWSYLQLPPVN
                     SAELIKLTDLRAGSPVVWFERGGDRLYACSYPRMQGCAPETYEFARDDGFWQVRSHQA
                     AGACTAAR"
     gene            complement(788..1357)
                     /locus_tag="VHE37_07120"
     CDS             complement(788..1357)
                     /locus_tag="VHE37_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVT35335.1"
                     /translation="MTWINARVVGQRQPSARNFRGDTMLGGFPIRSTGAGIERVPTNG
                     PQRPMAVDGERIEQLCAFIERLHWAALVANAEGEIVVANRGARSLFGLNGTECASIED
                     LVAADQRDAHRIYRQVYNSVPLQRPMGKYPRLRGQRADGTPLWVQIELVPFWTSAGVW
                     TLALLREAVEAGRHAASRRNLEVLGEHTV"
     gene            complement(1419..1492)
                     /locus_tag="VHE37_07125"
     tRNA            complement(1419..1492)
                     /locus_tag="VHE37_07125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1458..1460),aa:Gln,seq:ctg)
     gene            complement(1582..1989)
                     /gene="rpsI"
                     /locus_tag="VHE37_07130"
     CDS             complement(1582..1989)
                     /gene="rpsI"
                     /locus_tag="VHE37_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HVT35336.1"
                     /translation="MAKAANKVSYNTGTGRRKTAAARVFLKPGKGDITINGKTVEDYF
                     GRETSRMLVRQPLEISDAVSRFDIKVTVAGGGPSGQAGAVRHGITRALIDYDESLRKA
                     LRVAGLVTRDAREVERKKVGFHKARRGTQYSKR"
     gene            complement(1993..2427)
                     /gene="rplM"
                     /locus_tag="VHE37_07135"
     CDS             complement(1993..2427)
                     /gene="rplM"
                     /locus_tag="VHE37_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HVT35337.1"
                     /translation="MKTVFINGHDAKRDWCLVDAEGVVLGRLATEVARRLRGKHKPEF
                     TPNADNGDYVVVINAEKVKATGNKLNGKIYYSHSQFPGGIKQVTLGQVLATHPERAIE
                     KAVKGMLPKGPLGYAQFRKLKVYAGPEHPHTAQQPVKITVKP"
     gene            complement(2699..3760)
                     /locus_tag="VHE37_07140"
     CDS             complement(2699..3760)
                     /locus_tag="VHE37_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVT35338.1"
                     /translation="MVKILPYLKLAVEKSASDVFFTTNAPAMLKVEGEMMAIGKTMMT
                     YEFIKELAYSILTPEQQEYLEKNLEIDLATYAGGLGRFRVNIFTQRNNLSMVLRYVRG
                     DIPVLDDLKMPEVLKDLIMQKRGLVLMVGATGSGKSTTLAAMINHRNDNAAGHILTIE
                     DPIEFVHPNRRSIVNQREVGSDTISYERALKSALREAPDVILIGEIRTRETMESAMQL
                     ANTGHLVLATLHANNAFQALQRVVNLYPDQLRDQLYMDLALTLRSIISQRLVRRKDGK
                     RCAANEVMITTPFISDLILNRRIDEIKEAMNTSSDKGMLTFDNSLFKLWKDGLIDMDE
                     ALSNADSHANLEAKIVFGG"
     gene            complement(3911..>4596)
                     /locus_tag="VHE37_07145"
     CDS             complement(3911..>4596)
                     /locus_tag="VHE37_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVT35339.1"
                     /translation="IVTIEDPIEYVHPHVGCMITQREVGSDTQSWENALKNTLRQAPD
                     VILIGEIRTRETMEYAINFAETGHLVLSTLHANSANQAMDRIINFFPEEKREQLLMDL
                     SLNLKSIISQRLVRREGGGRCVAIEILINSPLVSDLIFKGEVAEIKEVMARSNEQGMV
                     TFDQTLFNLFEQGHISFEEAIRNADSQNEVRLRIKLESKRARQNLLDDELVKKLQMKE
                     EEKAQLMRR"
BASE COUNT          726 a         1444 c         1509 g          917 t
ORIGIN      
        1 caaccgcaac gacctgcgtt gcggcgacgg ctggaaccag gaagatctgt cgatcttttc
       61 gcgcgaccag caggatgcgc cggacgacga cggtggccgc gccgtggaag gcttctgccg
      121 tccctatgtg cagcgcgcgc agggtgtgat cgagcgcgtg cactggtcgg agcacgagcg
      181 cgtgttcagc gccgcgctgc agctcgatcc cgcaatcccg ggcgagacgg aagtcttcct
      241 gccgcgacgc cttggcaacg cgtttgaact gcgcgttacc ggcactgccg actgccgcca
      301 tgtccccggc gagcagcgcg tttacctcag cgcgcgcagt gccggcaatg tcgccattga
      361 gatccgctgc aagggctgat cagcgcgccg cggtacatgc gccggcggcc tggtgcgagc
      421 gcacctgcca gaagccgtca tcgcgcgcga actcataggt ttccggcgca cagccctgca
      481 tgcgcggata gctgcatgcg tacagccggt cgccgccgcg ctcgaaccac accaccggcg
      541 agccggcgcg cagatcggtg agcttgatca gttccgccga atttaccggc ggcagctgca
      601 ggtaactcca gcgccgcccg gcctcggcgt ggcaggcgcc ggccggcgtg tggtcatcgg
      661 ccgcgcaggc ggccagcagc aggacagcaa gcagcggtga cagtggtttc atcgggcgac
      721 tttaggagtg ccggcgctcc acgtgcgctg ccatctgacg aacgcggcaa tcgggtcgcg
      781 ttgacgatca gacggtgtgt tcgcccagca cttccaggtt gcgccggctc gccgcgtggc
      841 gcccggcctc caccgcttcg cgcagcagcg caagggtcca cacgccggcg gaggtccaga
      901 acggaacgag ctcgatctgc acccacagcg gcgtgccgtc ggcgcgctgg ccgcgcagcc
      961 gcggatactt gcccatgggg cgctgcaggg gcaccgagtt gtagacctgg cgatagatgc
     1021 gatgcgcgtc gcgctggtcc gcagcgacca ggtcctcgat cgacgcgcac tcggtgccat
     1081 tgagtccgaa caggctgcga gcgccgcggt tggccaccac gatctcgccc tccgcattgg
     1141 caaccagcgc cgcccagtgc agccgctcga tgaaggcgca cagctgctcg atgcgctcgc
     1201 cgtcaaccgc catcggccgc tgcgggccgt tggtgggcac gcgctcgatg cccgcgccgg
     1261 tactcctgat gggaaagccg cccagcatgg tgtcaccccg gaaattcctg gcgctaggct
     1321 gccgctggcc gaccacgcgg gcgttgatcc aggtcaatgc gctcttcagg cccgcccatc
     1381 gcgcaaataa aaagccccgc tggcgcgggg cttggtgttg gctgggagac taggactcga
     1441 acctagataa acagagtcag aatctgttgt cctgccatta gacgatctcc caaaatcgag
     1501 agtccgcgca ttagacgctc tacagcgact cttgtcatca gcatggtctg cgctccccaa
     1561 cgagagcgca gacctgcgga atcaacgctt gctgtactgc gtaccgcggc gggccttgtg
     1621 gaagccgacc ttcttgcgtt ccacttcgcg cgcgtcgcgc gtcaccagtc cggccacgcg
     1681 cagggccttg cgcaggctct cgtcgtagtc gatcagggcg cgggtgatgc cgtggcgcac
     1741 cgcgccggcc tggccggagg gaccgccgcc ggctaccgtg accttgatgt cgaagcggct
     1801 gaccgcgtcg gaaatttcca gcggctggcg caccagcatg cgcgaggtct cgcggccgaa
     1861 ataatcctcg acagtcttgc cgttgatggt gatgtcgccc ttgccgggct tgaggaacac
     1921 gcgggcggcg gcggtcttgc ggcggccggt gccggtgttg tacgaaactt tgttagcggc
     1981 tttggccatg gcttagggct tcacggtgat cttgacgggc tgctgcgcgg tgtgcggatg
     2041 ctcggggccc gcatacacct tcagcttgcg gaactgcgcg taaccgagcg ggcccttggg
     2101 cagcatgccc ttgaccgcct tctcgatggc gcgttccggg tgggtggcca gcacctggcc
     2161 cagggtgacc tgcttgatgc cgcccgggaa ttgcgagtgg ctgtagtaga tcttgccatt
     2221 gagcttgttg ccagtggcct tcaccttctc ggcgttgatg acgaccacgt agtcgccatt
     2281 gtccgcgttg ggggtgaatt ccggcttgtg cttgccgcgc aggcggcgcg ccacctcggt
     2341 ggcgaggcgg ccgaggacga cgccctcggc atcgaccaga caccagtcac gcttggcgtc
     2401 atggccgttg ataaaaacgg tcttcatgtc acttccttcg gtatttatag ttcagtcgcc
     2461 cttgccgcac ctttacccag gcgacctccg gtccccggtc aagggggcgc gcattttagg
     2521 ggcgggcggc ggccgatgca accgccgttg atcaggcagc aactcccgcg tagagcgttg
     2581 tgcgcgaagg caggctgcac ggtgccgagc gcagcaaccg gagttgacat gcagtcgatg
     2641 aggattgcga gccccgccga cgtgcaaatt gccgagcgtg gcagctctac gcgggagttc
     2701 agccgccgaa gacgatcttg gcttccaggt tggcatggct gtcggcattg gacagggctt
     2761 cgtccatgtc gatcaggccg tccttccaca gcttgaacag ggaattgtcg aaagtcagca
     2821 tgcccttgtc cgacgaggtg ttcatggctt ccttgatctc gtcgatgcgg cggttgagga
     2881 tcaggtcgga gatgaacggc gtggtgatca tcacctcgtt ggcggcgcag cgcttgccgt
     2941 ccttgcgccg caccaggcgc tgcgaaatga tcgagcgcag cgtcagcgcc aggtccatgt
     3001 acagctggtc gcgcagctgg tccgggtaga ggttaaccac gcgctgcagc gcctggaagg
     3061 cgttgttggc gtgcagcgtg gccagcacca ggtggccggt gttggccagc tgcatggccg
     3121 actccatggt ttcgcgcgtg cggatttcgc cgatcaggat gacgtcgggc gcctcgcgca
     3181 gcgcgctttt gagcgcgcgc tcgtagctga tggtgtcgct gcccacctcg cgctggttga
     3241 cgatgctgcg ccggttggga tgcacgaact cgatcgggtc ctcgatggtg aggatgtggc
     3301 cggcggcgtt gtcgttgcgg tggttgatca tggctgccag cgtggtggac ttgcccgagc
     3361 cggtggcgcc caccatcagc accaggccgc gcttctgcat gatcagatcc ttcagcacct
     3421 ccggcatctt gaggtcatcc agcaccggga tgtcgccgcg cacgtagcgc agcaccatcg
     3481 acaggttgtt gcgctgggtg aagatgttga cgcggaagcg gcccaggccg ccggcgtagg
     3541 tggcgaggtc gatctccagg ttcttttcca ggtactcctg ctgctccggc gtcaggatgg
     3601 agtaggccag ctccttgatg aactcgtagg tcatcatggt cttgccgatg gccatcatct
     3661 cgccttccac cttgagcatg gccggcgcgt tggtggtgaa gaagacgtcc gaggcgctct
     3721 tctccaccgc gagtttcagg tacggaagaa tcttgaccat gcggatatgc ctcgttttgc
     3781 cctgaccgca ccctcgattt cgatgttata ttttattatc tgattttaat ttcagattat
     3841 aatttataac atcaaaacca ggggtgcggt tgcgtgattt ttcgggagtg aacctgccgc
     3901 tgcgcagcgc tcagcgccgc atcagctgcg ccttctcctc ttccttcatc tgcagcttct
     3961 tcaccagctc gtcgtccagc aggttctggc gggcgcgctt ggactccagc ttgatgcgca
     4021 ggcgcacctc gttctgcgaa tcggcgttgc ggatggcttc ctcgaagctg atgtggccct
     4081 gctcgaacag gttgaacagc gtctggtcga aggtgaccat gccctgctcg ttggagcgcg
     4141 ccatcacttc cttgatctcc gccacctcgc ccttgaagat caggtcggac accagcggcg
     4201 agttgatcag gatctcgatg gccacgcagc ggccgccgcc ctcgcgccgc accaggcgct
     4261 ggctgatgat ggatttcagg ttcagcgaca ggtccatcag cagttgttcg cgcttctcct
     4321 cggggaagaa gttgatgatg cggtccatcg cctggttcgc gctgttggcg tgcagcgtcg
     4381 acagcaccag gtgtccggtt tcggcgaagt tgatggcgta ctccatcgtc tcgcgggtgc
     4441 ggatttcgcc gatcaggatg acgtcgggcg cctggcgcag cgtgttcttc agcgcgttct
     4501 cccagctctg cgtatcgctg cccacctcgc gctgcgtgat catgcagccg acgtgcgggt
     4561 gcacgtactc gatcgggtcc tcgatggtga cgatat
//
LOCUS       DASZZC010000338         5352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_403853,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000338 DASZZC010000000
VERSION     DASZZC010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403853"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VHE37_07150"
     CDS             complement(<1..597)
                     /locus_tag="VHE37_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT35340.1"
                     /translation="MSAIKLVIGDKSRSSWSLRAWLFLRHHQVPFTEQVIALDRAETH
                     AEILKHSPSGRVPVLVHGQVHVWESSAICEYAAETFALPGAWPMTPASRALARSIAAE
                     MHAGFADLRRELPFDARRDPSPKDYSERTAADIRRICAIWREARPKSRGDGWLFGSFG
                     IVDAMYAPVALRLHAYAVKLDAGERDYVDQVLRHPAVES"
     gene            631..2352
                     /locus_tag="VHE37_07155"
     CDS             631..2352
                     /locus_tag="VHE37_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HVT35341.1"
                     /translation="MIARLLICALALAPLTATAGIKVQVEGISGDEKTNVMERLAIAD
                     AAARKDLDQALVERLHAQADDDIRKALQVYGWYEPQIKAELSGEAPDWTARYTVSLGA
                     PVLVDHVEILLTGEGAEFPQLVAASQHPLVHEGRQLEHRQYEDTKARLVQRALASGFL
                     DAQFTRAQLLVTPAEHRAEVLLTLDTGPRYYFGSFTVEQEGLQPELVERYVRITPGGP
                     YDPQQVLDTQFALADLDYFQSVQIIPQRDRMFENHIPILIKTTPRPLHHYETGAGYGT
                     DTGARITEGVEFRRLNRYGHKLRLNARVSQIKDVLGAEYRIPLGSKSSESMSFTAEST
                     REKFDDGRTLQYGVGASLNRTPGRWQRRVYLQYDHEESTLPLQEITADLVMPGVSFNR
                     GETDDPIHTRRGWNLFLDAHGADKDVLSTATFLQLHGTLRAAYPLTHRLRLYGRGELG
                     ATFASQFSELPISQRFYAGGDQSVRGYAYRSLGPRDALGNVVGGRYLTVGSIEADYRI
                     VGNWGAALFADAGGVQNNPWPSLSRGVGFGARYRAPVGDIDVDIAWPLDGDTRSPRLH
                     LGIRVGL"
     gene            2349..>5352
                     /locus_tag="VHE37_07160"
     CDS             2349..>5352
                     /locus_tag="VHE37_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HVT35342.1"
                     /translation="MKLRHLIFPQHPFKLLWRPLKLLLEALLPCLVGGLIFLVYTPSG
                     LALGVYVLQDLTHGELRVTGAHGSLRGPLQIDEFIYDTPVMTLRIEHAALDWNTYALL
                     RARLDVGSVQAQRVQLTLKRHPHTTPEKPALTHLPLALHVAQLQAQDFELYPQDSSRP
                     VLFRNIRLAANWRYDILDVHAVHADYAPLGPLDAALRLQLQPHGLEILESHWRAPAQA
                     QAQGSIGYDNHMKLRVDFAQLQWPFSGDALVAGSAGIATAAGTWQHYNWTLRGQVRGN
                     FQPQPIDAGLVAHGRGTLHGLEVQQAELRGKGGTLRAQAQLAWLPAFALSLQGEASGV
                     SPAIEWNEWAGNIAGKFQLDLQHADDGVRAQFSASLADSTLRDYRFALDTRGSYAAHV
                     LDFAQLDVQSGASRISVHGRATPPFDLAATIDSPDLANLLPQLSGSGQAQLAFAGTLA
                     EPHLSGHVQLAHAQYGTFRLDQATLDADLSPRLPSRIDLAAKNLIAGLLMPSVQLHGQ
                     GTAAAHQLQLKAVTQAGSLDLALRGALDTKALHWQGQVYESHGQPLRLSPWTLEEAAP
                     LEIYRGYVQLDPVCWRSSAARACTQFALRGSNARLAFRLQDWAFAYFEPFLPPQWTLG
                     GTASGSGLLVYEQGRLRSARADLDSAGGALNVSRHPALTFAASSLRISDEDAGTKAQL
                     HMPLANGGIELKATLAPAAADGTRALSGHVAADVGDLTPLRLLSPQLESIDGRLHGDF
                     DLAGTAADPLAQGVLQLIDGRVRLATPGLDVTGLQASLRGDSSSRLINVSASAQSGGG
                     SLALSGTINPRGAQERVDLHLSGQEFQVLNTPQMRVWASPELDFHAGADHVQLRGEVV
                     VPRAEIKAKSFSQGVAPSRDQVILGPDAQAPPEVTFKTDAQVRLTLGDAVSFEGFGLK
                     ALLRGGIDVIDQPGRPTQGRGEISLAEGRYKAYGQDLTIDSGRLLFEGGLITQPGIDV
                     RAVRNNTGDVTVGVLVRGTLAAPQLSLFS"
BASE COUNT          827 a         1895 c         1870 g          760 t
ORIGIN      
        1 cgattccacc gcaggatgtc gcagcacctg gtcgacgtaa tcgcgctcgc ccgcgtcgag
       61 cttcaccgcg taggcatgca ggcgcagcgc caccggcgcg tacatggcgt cgacgatgcc
      121 gaagctgccg aacagccagc cgtcgcccct ggacttgggc cgcgcctcgc gccagatggc
      181 gcagatgcgg cggatgtcgg cggcggtgcg ctccgaataa tccttgggcg acggatcgcg
      241 ccgcgcatcg aacggcagct cgcgccgcag gtcggcgaag ccggcgtgca tctcggcggc
      301 gatcgagcgc gccagcgcgc ggctggccgg cgtcattggc caggcgccgg gcagggcgaa
      361 ggtctcggcg gcgtattcgc agatggcgct ggattcccac acgtgcacct gcccgtggac
      421 cagcaccggc acgcggccgc tgggcgaatg cttgaggatt tcggcgtgcg tctcggcgcg
      481 gtcgagcgcg atgacctgct cggtgaacgg cacctggtga tgccggagaa acagccatgc
      541 gcgcagcgac cacgatgacc ggctcttgtc gccgatgacc agcttgatgg cggacatgtc
      601 gcgcgcacct catgcaaaaa tgcgggccgc atgatagccc ggctcctgat ctgcgctcta
      661 gcgctcgccc cgctcacggc gacggccggc atcaaggtgc aggtcgaagg catttccggc
      721 gacgagaaaa ccaacgtaat ggaacgcctg gccatcgccg atgccgccgc acgcaaggac
      781 ctggaccagg cgctggtcga gcgcctgcat gcgcaggccg acgacgacat ccgcaaggcg
      841 ctgcaggtct acggctggta cgagccgcag atcaaggcgg agctgagcgg cgaggcgccg
      901 gactggaccg cgcgatacac cgtttcactc ggtgcgccgg tgctggtcga ccacgtggag
      961 atcctgctca ccggggaagg cgccgagttc ccgcaactgg tggcggcgtc gcagcacccg
     1021 ctggtgcacg agggccgcca gctggagcac aggcaatacg aggacaccaa ggcgcgcctg
     1081 gtgcagcgcg cgctggccag cggttttctc gacgcacagt tcacccgtgc gcagctgctg
     1141 gtgacgccgg ccgagcaccg cgccgaggtg ctgctgacgc tcgacaccgg cccgcgctac
     1201 tacttcggca gcttcaccgt ggagcaggaa ggcctgcagc cggaactggt ggagcgctac
     1261 gtgcgcatca cgcccggggg tccgtacgat ccgcagcagg tgctggacac gcagttcgcg
     1321 ctggccgacc tcgactattt ccagagcgtg cagatcatcc cgcagcgcga ccgcatgttc
     1381 gagaaccaca tcccgatcct gatcaagacc acgccgcggc cgctgcacca ctacgagacc
     1441 ggcgccggct acggcaccga caccggcgcg cgcatcaccg agggtgtgga attccgccgc
     1501 ctcaaccgct acggccacaa gctgcggctg aacgcgcggg tgtcgcagat caaggacgtg
     1561 ctgggcgcgg aataccgcat cccgctgggc agcaagagca gcgagagcat gagcttcacc
     1621 gccgaatcca cccgcgagaa attcgacgac ggccgcacct tgcagtacgg cgtgggcgcc
     1681 tcgctcaacc gcacgccggg ccgctggcag cggcgcgtgt acctgcagta cgaccacgag
     1741 gaatccaccc tcccgctgca ggagatcacc gccgacctgg tgatgcccgg cgtgtcgttc
     1801 aaccgcggcg aaaccgatga cccgatccac acgcggcgcg gctggaacct gttcctcgat
     1861 gcccacggcg ccgacaagga cgtgctgtcc accgccacct tcctccagct gcacggcacg
     1921 ctgcgcgccg cctacccgct gacccaccgc ctgcgcctgt acggccgcgg cgaactgggc
     1981 gccaccttcg cctcgcagtt ttccgagctg ccgatctcgc agcgcttcta tgccggcggc
     2041 gaccaaagcg tgcgtggcta cgcctatcgc tcgctcggcc cgcgcgatgc gctgggcaac
     2101 gtggtgggcg ggcgctatct cacggtcggc tcgatcgagg ccgattaccg catcgtcggc
     2161 aactggggcg cggcgctgtt cgccgatgcc ggcggcgtgc agaacaaccc ctggccctcg
     2221 ctgtcgcgcg gcgtcggctt cggtgcgcgc taccgcgcgc cggtgggcga catcgacgtc
     2281 gacatcgcct ggccgctgga cggcgacacg cgcagcccgc gcctgcacct gggcatccgg
     2341 gtgggcctgt gaaactgcgc catctcatct ttccgcagca tcctttcaag ctgctgtggc
     2401 ggccgctcaa gctgctgctg gaggcgctgc tgccgtgcct ggtgggcggg ctgatcttcc
     2461 tggtctacac gccgtcgggg ctggcgctgg gcgtgtacgt gctgcaggac ctgacccacg
     2521 gcgagctgcg cgtcactggc gcgcatggct cgctgcgcgg cccgctgcag atcgatgaat
     2581 tcatctacga cacgccggtg atgacgctgc gcatcgagca tgccgcgctc gactggaaca
     2641 cctacgccct gctgcgcgcg cggctcgacg tgggcagcgt gcaggcgcag cgcgtgcagc
     2701 tgacgctcaa gcggcatccg cacaccacgc cggaaaagcc cgcgctcacg cacctgccgc
     2761 tggcgctgca cgtggcgcag ctgcaggcgc aggacttcga gctgtatccg caggacagca
     2821 gcaggccggt gctgttccgc aacatccgcc tggcggccaa ctggcgctac gacatcctcg
     2881 atgtgcacgc ggtgcatgcg gactacgcgc cgctcggtcc gctggatgcc gcgctgcgcc
     2941 tgcagctgca gccgcacggg ctggagatac tcgagtcgca ctggcgcgcg ccggcgcagg
     3001 cgcaggcgca ggggtcgatc ggctacgaca accacatgaa gctgcgcgtg gactttgcgc
     3061 agctgcaatg gccgttcagc ggcgacgcgc tggtggccgg ctcggccggc atcgccaccg
     3121 ccgcgggcac ctggcagcac tacaactgga cgctgcgcgg acaggtgcgc ggcaatttcc
     3181 agccgcagcc gatcgacgcc gggctggtcg cgcacgggcg cggcacgctg cacgggctcg
     3241 aggtgcagca ggccgaactg cgcggcaagg gcggcacgct gcgcgcccag gcgcagctgg
     3301 cctggctgcc ggcgttcgcg ctgtcgctgc agggcgaggc cagcggcgtg agccccgcaa
     3361 tcgaatggaa cgaatgggcc ggcaacatcg ccggcaaatt ccagctggac ctgcagcatg
     3421 ccgacgacgg cgtgcgcgcg cagttctcgg catcgctggc cgattcgacg ctgcgcgact
     3481 atcgcttcgc gctggacacg cgcggcagct acgccgcgca tgtgctcgat ttcgcgcagc
     3541 tcgacgtgca gtccggcgcc agccgcatca gcgtgcacgg ccgcgccacg ccgccgttcg
     3601 atctggcggc gaccatcgac agccccgacc tggccaacct gctgccgcaa ctctccggca
     3661 gcggccaggc gcagctggcg ttcgccggca cgctggcgga gccgcacctg tcgggccacg
     3721 tgcagctggc gcacgcgcag tacggcacgt tcaggctcga ccaggcgaca ctcgatgcgg
     3781 atctgagccc gcgactgcct tcgcgcatcg atctcgcggc aaaaaatctc attgccggat
     3841 tgttgatgcc ctcggtgcag ctgcatggac agggcacggc cgccgcacac cagctgcagc
     3901 tgaaggcggt gacgcaggcc ggttcgctcg acctggcgct gcgcggtgcg ctggatacga
     3961 aggcactgca ctggcaaggg caggtctacg aaagccatgg ccagccgctg cgcctgtcgc
     4021 cgtggacgct ggaagaagcc gcgccgctgg aaatctaccg cggctacgtg cagctcgacc
     4081 cggtgtgctg gcgcagttcc gccgcgcgcg cctgcaccca gtttgcgctg cgcggcagca
     4141 atgcgcgcct ggcgttccgc ctgcaggact gggccttcgc ctacttcgag ccgttcctgc
     4201 cgccgcagtg gacgctgggc ggcaccgcca gcggcagcgg cctgctggtc tacgaacagg
     4261 gcaggctgcg cagcgcgcgc gccgacctcg acagcgccgg cggcgcgctc aacgtgagca
     4321 ggcatcccgc gctgactttc gccgccagca gcctgcgcat cagcgacgag gacgcgggca
     4381 ccaaggcgca gctgcacatg ccgctggcca acggcggcat cgagctcaag gccacgctgg
     4441 cgccggcggc ggccgatggc acgcgcgcgc tgtcgggaca cgtggccgcc gatgtcggcg
     4501 acctgacgcc gctgcgcctg ctgtcgccgc agctggagtc gatcgacggc cgcctgcacg
     4561 gcgatttcga tctggccggc accgcggccg atccgctggc gcagggcgtg ctgcagctga
     4621 tcgatggccg cgtgcgcctg gccacgccgg ggctcgacgt caccggcctg caggccagcc
     4681 tgcgcggcga ttcgtcctcg cgcctgatca atgtgagcgc ctcggcccaa tccggcggcg
     4741 gcagcctggc gctgagcggc acgatcaatc cgcgcggcgc gcaggagcgc gtggacctgc
     4801 acctgagcgg gcaggaattc caggtgctga atacgccgca gatgcgcgtg tgggcctcgc
     4861 cggagctcga cttccacgcc ggcgcggacc acgtgcagct gcgcggcgag gtggtggtgc
     4921 cgcgcgccga gatcaaggcc aagtcgttct cgcagggcgt ggcgccgtcg cgcgaccagg
     4981 tgatcctggg ccccgacgcg caggcgccgc cggaggtgac cttcaagacc gacgcgcagg
     5041 tgcggctgac gctgggcgac gcggtgagct tcgagggctt cggcctcaag gcgctgctgc
     5101 gcggcggcat cgacgtgatc gaccagccgg gccggccgac gcagggccgc ggcgagatca
     5161 gcctggccga gggccgctac aaggcctatg ggcaggatct caccatcgac agcgggcgcc
     5221 tgctgtttga gggcgggctg atcacgcagc cgggcatcga cgtgcgcgcg gtgcgcaaca
     5281 acaccggcga cgtcaccgtg ggcgtgctgg tgcgcggcac gctggcggcg ccgcagctgt
     5341 cgctgttctc ca
//
LOCUS       DASZZC010000339         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_542617,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000339 DASZZC010000000
VERSION     DASZZC010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542617"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VHE37_07165"
     CDS             complement(<1..790)
                     /locus_tag="VHE37_07165"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HVT35343.1"
                     /translation="MSERLGTPMMRLLLIGLLLVLLQIPLGMIQGLIGERQARRNDAV
                     DEVMGKWGQRQSVNGPLLRIPYIHHVDLGGTPPRRVAQTEYAYFLPARLDIQSRLHSQ
                     LRRRGLFEVPVYDAQLTLSGHFDAPDWTALHVAVEDIDLKDAELVLGISDVRAVHADV
                     HLLWDGQELPFRPSGGADAEWKFSGVHAPLQDAAQTLARSDGVEFRITAGINGSVALD
                     FAPMAEQTTAMVAADWPHPSFDGAWLPTQHQEQDGGFSARWSVSY"
     gene            complement(831..1259)
                     /locus_tag="VHE37_07170"
     CDS             complement(831..1259)
                     /locus_tag="VHE37_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35344.1"
                     /translation="MRGNILLRPSNLLLLCGLILLALTAMLSACGAPAALCLGCAAFA
                     HAGEQLPGSETNLRYTACLLVASATLRLFGQVLRMPALSATPRMLEAYALRTLAGLHQ
                     LQPQAPRRWGGAIYCGSLALERASQRLTGAALQQLLGLHI"
     gene            1412..2104
                     /locus_tag="VHE37_07175"
     CDS             1412..2104
                     /locus_tag="VHE37_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT35345.1"
                     /translation="MQKILIVDDDAHIREVVSFALGKAGFTVVTAENGRQALEKFQRE
                     RPALLVLDILMPELDGTEVCRQLRAQPSGRATPIIFLSSKDDEVDRIVGLELGGDDYL
                     AKPFSPRELVARVRALLRRAGPQSQATEGARMAHGRLALDPELFKAWWGEQEVVLTLT
                     EFGVLRTLLQRPGKVCTRDHLMDASYELHKIVSDRTIDSHVRRVRSKFAAIGADPIET
                     VHGVGYKLGPCA"
     gene            2127..>3628
                     /locus_tag="VHE37_07180"
     CDS             2127..>3628
                     /locus_tag="VHE37_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVT35346.1"
                     /translation="MKRPRLRSLLILINLLVLALPLTSIEVLRIYESALVRQTESELI
                     AQGVVIAAAYRSFFNRFAAKADLAGYGVPRTAADDTLKPGEPWQPRNPQLDLASDPIF
                     PPPPDPVPAAQTPDSYARNAALELAPIIREAQFVTLAGIRVTDAHGVIVASTGEDRGM
                     SVAHYEEVRRALTGEYVSLMRERISDQPQPALDSISRGTRIRIYSAIPILRGDRVIGA
                     VLLVRTPSNIRRAIWGKRDTLLRGGLLLLAAVFVLGSLSALMISRPVNALMAQARRAA
                     RGEQGAITPLRHPGTREIAELSDTIAAMAQAMEQRANYIRDFAAHVSHEFKTPLTAIQ
                     GSIELLRDHAAAMSDEERTRFFDMITADARRLEQLVRRLLELARADVMKVGSEQCDVA
                     AVLPAAAARYRERGLDLELSGQPAPCRVAMAAETLDSIVSNLLDNARQHAGENVRVRL
                     SWSQPSAQQVAISVSDNGSGISAANAQRIFEPFFTTARKAGNTGLGLPIIR"
BASE COUNT          588 a         1283 c         1215 g          542 t
ORIGIN      
        1 gatacgacac tgaccagcgc gcggaaaatc ctccgtcctg ttcctggtgc tgcgtcggca
       61 accacgcgcc atcgaacgac ggatgcggcc aatccgccgc gaccatggcg gtggtctgct
      121 cggccatcgg cgcgaagtcc agcgccacgc tgccattgat gcctgcggtg atgcggaact
      181 cgacgccatc gctccgcgcc agcgtctgcg ccgcatcctg gagcggagcg tgcacgcccg
      241 aaaatttcca ctccgcgtcg gcgccgccgg aaggccggaa cggcagttcc tgcccgtccc
      301 atagcaggtg cacgtcggca tgtaccgcgc gcacatcgga aatgcccagc accagctcgg
      361 catccttgag gtcgatgtct tcgactgcca cgtgcagcgc cgtccagtcg ggcgcatcga
      421 aatgcccgga caaggtcagc tgcgcgtcgt acaccggcac ttcgaacagg ccgcgccggc
      481 gcaactggct gtgcaggcgc gactggatgt ccagccgcgc cggcaggaag tacgcgtact
      541 cggtctgtgc cacgcggcgc ggcggtgtcc cgccgagatc cacgtgatgg atgtagggaa
      601 tgcgcagcag cggaccgttc acgctctggc gctgacccca tttgcccatc acctcatcga
      661 ccgcgtcgtt gcgccgcgcc tgccgctcgc cgatcagccc ctggatcatg cccagcggaa
      721 tctgcagcag caccagcagc agcccgatca gcagcagccg catcatcggc gtgcccaggc
      781 gctcgctcat cggattgctc gtcggttgcg ccataatgct tttctccgtt tcagatgtgc
      841 aggcccagca gctgctgcag ggcagcgccg gtaagccgct gcgatgcgcg ttccagtgcc
      901 agcgagccgc aatagatggc tccgccccag cgccgcggcg cctgcggttg cagttggtgc
      961 agcccggcca gggtgcgcaa ggcatacgcc tcgagcatgc ggggagtggc ggagagcgcg
     1021 ggcatgcgca gcacctggcc gaagagccgc agggtcgcgc tcgccaccag caggcaggcg
     1081 gtgtagcgca gattggtttc gctgccgggg agctgttcgc cggcatgggc aaaggcagcg
     1141 cagcccaggc acaacgccgc cggagcgccg caggcactga gcatggcggt cagcgccagc
     1201 aggatcaggc cgcacagcag cagcaggttc gatggccgca gcaggatgtt tccgcgcatg
     1261 ctttgctccg aggcaagtct cgcttgcgat gcgctgcagt ctgcggacag gacttgcaag
     1321 gcctgcggcc atgccgtgca ggagtcgagg aacttggtgc aggatttgtg cagccgcacc
     1381 gccacgctgg gcgcgctgct aaagtggctg catgcagaaa atcctcatcg tcgacgacga
     1441 cgcgcatatc cgcgaagtgg tgagcttcgc gctgggcaag gccggtttca ccgtcgtcac
     1501 ggccgagaat ggacgccagg cgctggagaa gttccagcgc gaacggcccg cgctgctggt
     1561 gctcgacatt ctcatgcctg aactcgacgg caccgaggtc tgccgccagt tgcgcgcgca
     1621 gccgtccggc cgcgccacgc cgatcatctt tctatcgtcg aaggacgacg aggtcgaccg
     1681 catcgtcggc ctggaactgg gcggcgacga ttacctggcc aagccattct cgccgcgcga
     1741 gctggtggcg cgcgtgcgcg cgctgctgcg ccgcgccggg ccacagtcgc aggccaccga
     1801 gggcgcgcgc atggcgcacg gcaggctggc gctggacccg gaactgttca aggcctggtg
     1861 gggcgaacag gaagtggtgc tgacgctcac cgagttcggc gtgctgcgca cgctgctgca
     1921 gcgcccgggc aaggtgtgca cgcgcgatca cctgatggac gcatcgtacg aactgcacaa
     1981 gatcgtctcc gaccgcacca tcgacagcca cgtgcgacgc gtacgcagca agttcgccgc
     2041 catcggcgcc gacccgatcg agacggtgca cggcgtcggc tacaagctcg gcccctgcgc
     2101 ctgattccgc gcgccgcgca cgcccgatga agcgcccgcg gctgcgcagc ctgctgatcc
     2161 tgatcaacct gctggtgctg gcattgccgc tgaccagcat cgaggttctg cgcatctacg
     2221 agagcgcgct ggtgcgccag accgaatcgg agctgatcgc gcagggcgtg gtcatcgccg
     2281 cggcctaccg ttcgttcttc aaccgctttg ccgccaaggc cgacctcgcc ggctatggcg
     2341 tgccgcgcac cgcggccgac gacacgctga agccgggcga gccgtggcag ccgcgcaatc
     2401 cgcagctcga cctggccagc gatccgatct tcccgccgcc gcccgatccc gtgccggcgg
     2461 cgcaaactcc cgatagctac gcgcgcaacg cggcgctcga actcgcgccg atcatccgcg
     2521 aggcgcagtt cgtcaccctg gccggcatcc gcgtcactga tgctcacggc gtcatcgtcg
     2581 ccagcaccgg cgaggaccgc ggcatgtcgg tggcgcatta cgaggaagtg cggcgcgcgc
     2641 tgaccggcga atacgtcagc ctgatgcgcg agcgcatctc cgaccagccg cagccggcgc
     2701 tggactcgat cagccgcggc acgcgcatcc gcatctacag cgccatcccc atcctgcgcg
     2761 gcgaccgcgt gatcggcgcg gtgctgctgg tgcgcacgcc gtcgaacatc cgtcgcgcca
     2821 tctggggcaa gcgcgacacg ctgctgcgcg gcggcctgct gctgctcgcc gcggtcttcg
     2881 tgctcggctc gctgtcggcg ctgatgatca gccggccggt aaacgcgctg atggcgcagg
     2941 cgcggcgcgc ggcgcgcggc gagcagggcg ccatcacgcc cctgcgccac cccggcacgc
     3001 gcgagatcgc cgaactgtcc gatacgattg ccgccatggc ccaggcaatg gaacagcgtg
     3061 ccaactacat ccgcgatttt gcggcgcacg tgtcgcacga gttcaagacc ccgctgacgg
     3121 cgatccaggg ctcgatcgaa ctgctgcgcg accacgccgc ggcgatgtcc gacgaggagc
     3181 gcacgcgctt cttcgacatg atcaccgccg atgcacggcg gctggagcag ctggtgcgcc
     3241 gcctgctcga actggcgcgc gccgacgtga tgaaggtcgg cagcgagcag tgtgacgtcg
     3301 ccgcggtgct gcccgccgcc gcggcgcgct accgcgaacg cggcctggac ctcgagctct
     3361 ccggccagcc ggcgccgtgc cgcgtggcga tggcggccga gacgctcgat tccatcgtca
     3421 gcaacctgct ggacaacgcg cgccagcacg ccggggaaaa cgtgcgcgtg cggctgagct
     3481 ggtcgcaacc ttcggcgcag caggtggcga tcagcgtcag cgacaacggc agcggcattt
     3541 ccgctgccaa cgcgcagcgc atcttcgagc cgtttttcac caccgcgcgc aaggccggca
     3601 acaccggcct gggcctgccg atcatccg
//
LOCUS       DASZZC010000340         4115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_281315,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000340 DASZZC010000000
VERSION     DASZZC010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281315"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHE37_07185"
     CDS             complement(<1..227)
                     /locus_tag="VHE37_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HVT35347.1"
                     /translation="MNNVYGYSAPVWARFENPARAGRLDGAGVVTVQAGTVAAKSLLQ
                     VDLRIDRGKVSEARFLAYGCPVTIAVGDWLA"
     gene            complement(224..1390)
                     /locus_tag="VHE37_07190"
     CDS             complement(224..1390)
                     /locus_tag="VHE37_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVT35348.1"
                     /translation="MIYLDNAATTPLDPAVREAMLPLLDPLGALYANPSSAHVAGQAA
                     AHVVTQARAQVAQLVGAQDDEIIFTSGATESNNLAIKGALEFYGGRGTQHVITSKTEH
                     RCVLDTCRYLETRDVRVTYLKPGHGGVVSAQQVLDALTPDTRLVSLMWVNNEIGTINQ
                     IEALAPQLRGRGVMLHVDAVQAAGKIPVDMAQVPVDLLSFTAHKLHGPKGIGALYVRK
                     RPRARLAPQLHGSGHEQGMRSGTLATHQIAGFGAACEVARVKLAEDGRRLGALRDELW
                     LRLSALPRAVRNGDPAASAPHILNVSFDGAEGESLRALLPELAVSSGSACSSATREPS
                     FVLRALGHDDELAGSSLRFSLGRMTSAADIAAAADMVVAAVTRLRALNPRWSAA"
     gene            complement(1387..2511)
                     /locus_tag="VHE37_07195"
     CDS             complement(1387..2511)
                     /locus_tag="VHE37_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HVT35349.1"
                     /translation="MAAYLDHNASTRLDARVLEAMLPYLSGPYGNASSLHRFGRAARD
                     AVETARAQVARLVNAQPQEIVWTSGGTESNNLALKGSIAGAAPSRVLYGATDHPAVME
                     AAESLRAAGWSVEPVAVDRAGVIDWNAYQAQLGRGPARLVTIMRANNETGVLQDVARA
                     GALAHAHGARMHVDAVQAAGKIAVDFAALGCDLLSLSSHKIYGPKGIGALVVKAGVEL
                     HPLHHGGPQERGLRGGTENVAAIAGFGAAAELALAELDARAAHALALREQLEAGLRGL
                     AGITIFGERSPRLPNTVQFALAGYDGEALLMQLDRKGMAVSSGSACASGNHEPSHVLL
                     AMGYPREVALGAIRVSLGKDNTPAEIGQFLAALTSLAPGR"
     gene            complement(2517..3329)
                     /gene="cysE"
                     /locus_tag="VHE37_07200"
     CDS             complement(2517..3329)
                     /gene="cysE"
                     /locus_tag="VHE37_07200"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HVT35350.1"
                     /translation="MFSRIREDIASVFDRDPAARTTWEVLTCYPGLHAVWGHRISHWL
                     WTHGLRWLARFNSHLARWLTGIEIHPGAKIGRRLFIDHGMGVVIGETAEIGDDVTLYH
                     GVTLGGVSWNQGKRHPTLANNVVVGAGAKVLGPFTVGEGARVGSNSVVVKEVPPGATV
                     VGIPARIVSEQRAPSDKVVAIARKMGFDAYGLSRDMPDPVANAIAAMLDHIQKQDEQL
                     VRVCKTLHQIDSRMPELKLEKLSLPDFCKDAGEVLDPVDSFAAAKPKDRKAG"
     gene            complement(3498..>4115)
                     /locus_tag="VHE37_07205"
     CDS             complement(3498..>4115)
                     /locus_tag="VHE37_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HVT35351.1"
                     /translation="RAQSAGAIAVLERARVVETLDEAIADCGWVVATSARPRHLGDEP
                     LPPPAFAARAVELLPSARVALVFGCERTGLTNEELDRCQARTLIPSNPEYSSLNLAQA
                     VQLYTWELRKAVLPDAPRVSGKQDNPVYAPPSAQEFEHYFEHLEKVLLATGFLDPENP
                     RALMRRLRQFYGRAAPDRNELNMLRGILTSFERPKIRKKPPTAPG"
BASE COUNT          630 a         1430 c         1407 g          648 t
ORIGIN      
        1 tccgccagcc agtcgccgac cgcaatcgtt acggggcagc cgtaggcgag gaaacgggct
       61 tcgctcacct tgccgcggtc gatgcgcaag tccacctgca gcagcgactt cgccgccacg
      121 gtcccggcct gcaccgtcac cacgccggcg ccatccagcc gcccggcgcg cgccgggttt
      181 tcgaagcgcg cccataccgg cgcggaatag ccgtaaacgt tgttcatgcc gcgctccagc
      241 gcgggttcag cgcccgcagg cgggtgacgg cagcgaccac catgtccgct gccgcggcga
      301 tgtcggcggc gctggtcatg cgcccgagcg agaagcgcag cgaggagccg gcgagttcgt
      361 cgtcgtgtcc cagcgcgcgc agcacgaacg aaggctcgcg cgtggccgat gagcaggcgc
      421 tgccgctgga tacggccagt tcgggcagca gggcgcgcag gctttcgcct tccgcgccgt
      481 cgaagctgac gttgaggata tgcggtgcgc tcgccgccgg atcgccattg cgcaccgcgc
      541 gcggcagcgc tgacaggcgc aaccacaact cgtcgcgcag cgcgcccaat cgcctgccgt
      601 cttcggcgag cttcacgcgt gcgacttcgc aggccgcgcc gaagccggcg atctggtgcg
      661 tggccagcgt gcccgagcgc atgccctgct catggccgct gccgtgcagc tgcggggcca
      721 agcgtgcgcg cggccgcttg cgcacgtaca gcgcgccgat gcccttgggt ccgtgcagct
      781 tgtgcgccgt gaacgagagc agatcgaccg gaacctgcgc catgtcgacc gggattttcc
      841 cggccgcctg caccgcgtcc acgtgcagca tcacgccgcg cccgcgcagc tgcggcgcca
      901 gcgcttcgat ctggttgatg gtgccgatct cgttgttcac ccacatcagc gagaccagcc
      961 gcgtgtcggg cgtgagcgcg tcgagcacct gttgcgcaga caccacgccg ccgtgcccgg
     1021 gcttcagata cgtcacccgc acgtcgcgcg tttccaggta acggcaggtg tcgaggacac
     1081 agcgatgctc ggtcttcgag gtgatcacat gctgcgtgcc gcggccgcca tagaactcca
     1141 gcgcgccctt gatcgccagg ttgttcgact cggtggcgcc ggaagtgaag atgatctcgt
     1201 cgtcctgcgc accgaccagc tgtgccacct gcgcgcgcgc ctgcgtcacc acgtgcgccg
     1261 cggcctgccc ggccacgtgc gcggacgacg ggttggcata cagcgcgccg agcggatcga
     1321 gcagcggcag catggcctcg cgcaccgccg gatcgagcgg cgtggtcgcc gcgttgtcga
     1381 gatagatcat cggccgggcg ccagcgaggt gagcgcggcg agaaactgcc cgatctcggc
     1441 cggggtgttg tccttgccca gcgacacacg gatggcgccg agcgccactt cgcgcggata
     1501 gcccatcgcc agcagcacgt ggctgggctc gtggttgccg ctggcgcagg ccgagccgct
     1561 ggacacggcc atgcccttgc ggtcgagctg catcagcagc gcctcgccgt catagccggc
     1621 cagcgcaaac tgcacggtgt tgggcaggcg cggcgagcgc tcgccgaaaa tggtgatgcc
     1681 ggccaggccg cgcaggccgg cttcaagctg ctcgcgcagc gccagcgcgt gcgcggcgcg
     1741 cgcgtcgagt tcggccagcg cgagctcggc cgccgcgccg aagccggcga tggcggccac
     1801 gttctcggtg ccgccgcgca gcccgcgctc ctgcgggccg ccgtgatgca gcggatgcag
     1861 ttccacgccc gccttcacca ccagcgcgcc gatgcccttg gggccgtaga tcttgtgact
     1921 cgacaggctc agcaggtcgc agcccagcgc ggcgaaatcg accgcgattt tcccggcggc
     1981 ctgtaccgca tcgacatgca tgcgcgcgcc gtgcgcatgc gccagcgccc ccgcgcgcgc
     2041 cacgtcctgc agcacgccgg tttcgttgtt ggcgcgcatg atggtgacca ggcgcgccgg
     2101 gccgcgcccc agttgcgcct ggtaagcgtt ccagtcgatg acgcccgcgc ggtcgaccgc
     2161 gaccggctcg accgaccagc ccgcggcgcg cagcgactcg gccgcttcca tcaccgccgg
     2221 atgatcggtg gcgccgtaga gcacgcgcga cggcgccgcc ccggcaatgc tgcccttgag
     2281 cgcgaggttg ttcgactcgg tgccgccgga ggtccagacg atttcctgcg gctgcgcgtt
     2341 gaccagccgc gccacctgcg cgcgcgccgt ctccaccgca tcgcgcgcgg cgcggccgaa
     2401 gcggtgcagc gaggaggcgt tgccgtaggg accggacagg tacggcagca tggcctcgag
     2461 cacgcgggcg tcgagccgcg tgctcgcgtt gtggtccagg tacgccgcca tcgggatcaa
     2521 cccgccttgc ggtccttggg cttggccgcg gcgaaggaat cgaccggatc aaggacttcg
     2581 ccggcgtctt tgcagaagtc cggcagcgac agtttttcca gcttgagctc gggcatgcgc
     2641 gaatcgatct ggtgcagcgt cttgcacacg cgcaccagct gctcgtcctg cttctggatg
     2701 tggtcgagca tggcggcgat ggcattggcc accgggtcag gcatgtcgcg cgacaggcca
     2761 tatgcgtcga accccatctt cctggcgatg gcgacgacct tgtccgacgg cgcgcgctgc
     2821 tccgacacga tgcgcgcagg aatgccgacc acggtggcgc cgggcggcac ttccttcacc
     2881 accacggaat tcgaaccgac gcgcgcgccc tcgcccacgg taaacgggcc gagcaccttg
     2941 gcgccggcac cgaccaccac gttgttggcc agcgtgggat ggcgcttgcc ctgattccac
     3001 gacacgccgc cgagcgtgac gccgtggtac agcgtgacgt cgtcgccgat ctctgcggtt
     3061 tcgccgatga ccacgcccat gccgtgatcg atgaacaggc gccggccaat cttcgcgccc
     3121 gggtggatct cgatgccggt gagccagcgc gcgaggtgcg aattgaagcg cgccagccag
     3181 cgcaggccat gcgtccacag ccagtgtgaa atccggtggc cccacaccgc gtgcagcccc
     3241 gggtagcagg tgagcacttc ccaggtcgtg cgcgccgcgg ggtcgcggtc gaatacgctg
     3301 gcgatgtctt ctcggatgcg ggagaacatg gaaggctcgg gacgggctcg gtctaagcgt
     3361 caattctact ccgcgcacag accacgccga cggaaaaacg tgccgcgggc ggccgaaaaa
     3421 cacctcaccg caccgccatt tttgaggtta taaattataa tatgttttta taattattaa
     3481 attatttgta acatcattca accgggtgcg gtaggaggct ttttgcggat tttcgggcgc
     3541 tcgaacgagg tgaggatgcc gcgcagcata ttcagctcgt tccggtccgg cgctgcgcgg
     3601 ccgtagaact ggcgcaggcg gcgcatcagc gcgcgcggat tttccggatc caggaaaccg
     3661 gtggcgagca gcactttctc caggtgctcg aagtaatgct cgaactcctg cgcgctcgga
     3721 ggcgcgtaca ccgggttgtc ctgcttgccc gatacgcgcg gcgcgtcggg cagcacggcc
     3781 ttgcgcaatt cccatgtgta cagctgcact gcctgcgcca ggttgagcga ggaatattct
     3841 ggattcgaag gaatcaaggt gcgcgcctgg cagcggtcga gctcttcgtt ggtcaggccg
     3901 gtgcgctcgc agccgaacac cagcgccacg cgcgccgacg gcagcagttc caccgcgcgc
     3961 gccgcgaacg ctggcggcgg cagcggctca tcgccgaggt ggcgcggacg ggccgaggtg
     4021 gcgaccaccc agccgcaatc ggcgatggct tcatcgagcg tttccaccac gcgcgcgcgt
     4081 tcgagcacgg cgatggcgcc ggccgactgc gcgcg
//
LOCUS       DASZZC010000341         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_476438,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000341 DASZZC010000000
VERSION     DASZZC010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476438"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /gene="aspS"
                     /locus_tag="VHE37_07210"
     CDS             complement(<1..1069)
                     /gene="aspS"
                     /locus_tag="VHE37_07210"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HVT35352.1"
                     /translation="MMRTHYCGQVTEALDGQTVSVCGWVHRRRDHGGVIFIDLRDRAG
                     LLQCVFDPDTKEAFEAADKLRGEYVVRVSGRVRPRPAGTENPNLPSGRIEVLGKSVEL
                     LNKSETPPFHPDDETVGEETRLKYRYIDLRRPQMQKNLMLRNRVVKAIRDFMDAEGFV
                     DVETPILTKATPEGARDYLVPSRTHPGQFFALPQSPQLFKQLLMMSGLDRYYQIARCF
                     RDEDLRADRQPEFTQLDVETSFLSMEEIMDIIERLVVGLFRNTLGVDLGKLPHMSYAE
                     AMARYGSDKPDLRNPLELVEVKELVKDVDFKVFSGPANDAKSRVAALRVPGGKDKLSR
                     GQIDGYTEFVKQYGARGLAYII"
     gene            complement(1256..1882)
                     /locus_tag="VHE37_07215"
     CDS             complement(1256..1882)
                     /locus_tag="VHE37_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HVT35353.1"
                     /translation="MSGTLLRRWVVAGLLVWIPLGVTLFVLSFLVRVLDASQVLIPQT
                     WRFPGLGIFLSALLVLGTGALTANLIGKRIFSWFELVLQRVPLLGSVYAGMKKLAETL
                     FSSTGASFRRVLLIEYPRKGMWTLAFQTGATAAELGHKTGRELLTVFVPTTPNPTSGF
                     VILVPSDEAIVLDMPVEDGLRLVISLGVVTPEAGAKKKEEIAPFGKLG"
     gene            complement(1879..2289)
                     /locus_tag="VHE37_07220"
     CDS             complement(1879..2289)
                     /locus_tag="VHE37_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVT35354.1"
                     /translation="MLRQRCQFEGPMPIYEYVCRACGKESEIIQKISDPPEKKCPLCG
                     KRQLQKLVSAAGFRLKGGGWYETDFKSGDKKKNVAGETKPEDSKSEPAKADNKPAEKT
                     PKSDKSEKSDKSDKSDKSEKKNKPADKPSKKAAA"
     gene            complement(2338..>3381)
                     /locus_tag="VHE37_07225"
     CDS             complement(2338..>3381)
                     /locus_tag="VHE37_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35355.1"
                     /translation="KKTLRISFNWAVGLFAAFILLCGITHVLDIITVWHPIYYVQGLV
                     KAATGIVSILTAIAIIPLVPRLARMRSPEELEEANHKLSDEVARRSIAETNLRQSVAE
                     LKAAMGELESFTYITSHDLQAPLRNIAGFSQLLSSRYRSRLDGDALEFLSYIDQGSRQ
                     MQQLIRDLLQLSRVGRSDNNFEVRPLDYSIGRARAALASEIEKSGAEIVHDPLPEILA
                     EHSLLTQLFQNLIGNSIKFQRPGNRPRIQIVCARSGSGWFISLSDNGIGIPANQLDQV
                     FAIFRRLHNADEFEGTGIGLAICRKIVTHHGGEIRAVKREGGAEFHIILPAEPSVARP
                     ALDEPVLEPGSAT"
BASE COUNT          560 a         1068 c         1105 g          648 t
ORIGIN      
        1 caatgatgta agcgagaccg cgtgcgccgt actgcttgac gaactcggtg tagccgtcga
       61 tctgcccgcg cgagagcttg tccttgccgc cgggcacgcg cagcgcggcc acgcggctct
      121 tggcatcgtt ggccgggccg gagaacacct tgaagtcgac gtccttcacc agctccttca
      181 cctcgaccag ctcgagcggg ttgcgcaggt cgggcttgtc cgaaccgtag cgcgccatcg
      241 cctcggcata gctcatgtgc ggcagcttgc ccaggtcgac gccgagcgtg ttcctgaaca
      301 agccaaccac caggcgctcg atgatgtcca tgatctcttc catggacagg aacgaggtct
      361 cgacgtcgag ctgggtgaat tccggctggc ggtcggcgcg caggtcttcg tcgcggaagc
      421 agcgcgcaat ctggtagtag cggtccagcc cgctcatcat cagcagctgc ttgaacagct
      481 gcggcgactg cggcagcgca aagaactggc cgggatgcgt gcgcgaggga acgagatagt
      541 cgcgcgcgcc ttccggcgtg gccttggtca ggatcggcgt ctcgacatcg acgaatcctt
      601 ccgcatccat gaagtcgcgg atcgccttga ccacgcggtt gcgcagcatc aggttcttct
      661 gcatctgcgg gcggcgcagg tcgatgtagc ggtacttcag ccgcgtttct tcgcccacgg
      721 tttcgtcgtc ggggtggaac ggcggcgttt ccgacttgtt caacagttcg acgctcttgc
      781 ccagcacttc gatcctgccg ctcggcaggt tcgggttctc ggtcccggcc gggcgcgggc
      841 gcacgcggcc gctgacgcgc accacgtatt cgccgcgcag cttgtcggcg gcctcgaacg
      901 cttccttggt gtccgggtcg aacacgcatt gcagcagccc ggcgcggtcg cgcaggtcga
      961 tgaagatgac gccgccgtgg tcgcgccggc gatgcaccca gccgcacacg ctcacggtct
     1021 ggccgtcaag cgcctcggta acctggccgc agtaatgggt gcgcatcatg gtgtgtggat
     1081 ggtgctcgga gggctggaaa acatgctcac cgcccccgct gtggaggcgt tatattttat
     1141 tgtccaaaag acaaatcgat cgacatttcg gaacacgcaa aatgccagtg cggtgtgccg
     1201 aaaaaggcgc gtagtttaaa cgaaatccgc gccgattcgg ccgcgcgcgg cgcgcttaac
     1261 ccagcttgcc gaacggcgcg atttcttcct tcttcttggc accggcttcc ggcgtgacca
     1321 cgcccagtga aatgaccagg cgcagtccgt cttccaccgg catgtcgagc acgatggcct
     1381 cgtcgctcgg caccaggatg acgaagccgg aggtggggtt gggcgtggtg ggcacgaaca
     1441 cggtgagcag ctcgcgcccg gtcttgtggc caagctcggc ggcggtggcg ccggtctgga
     1501 aggccagcgt ccacatgccc ttgcgcgggt actcgatcag cagcacgcgg cggaacgatg
     1561 cgccggtgga agagaacagg gtctccgcca gcttcttcat gcctgcgtag accgagccga
     1621 gcagcggcac gcgctgcaac accagttcaa accagctgaa gatgcgcttg ccgatcaggt
     1681 tggcggtgag tgcgccggtg cccagcacca gcagcgccga aagaaagatg cccaggcccg
     1741 ggaagcgcca ggtctgcggg atcagcacct ggctggcatc gagcacgcgc accaggaagc
     1801 tgagcacgaa cagcgtgaca cccagcggaa tccacaccag caggccggcg accacccagc
     1861 ggcgcagcag cgtgccgctc atgcagcggc cttcttcgac ggcttgtcgg cgggcttgtt
     1921 cttcttctcg gatttgtcgg atttgtcgga tttgtcggac ttctcggact tgtcggactt
     1981 gggcgtcttc tccgccggct tgttgtcggc cttggccggt tcgctcttgc tgtcctcggg
     2041 cttggtctcg ccggccacgt ttttcttctt gtcgccggac ttgaagtcgg tttcgtacca
     2101 gccgccgccc ttcaggcgaa agccggccgc cgacaccagc ttctgcaact ggcgcttgcc
     2161 gcacagcggg catttcttct ccgggggatc ggagattttc tggatgatct cggattcctt
     2221 gccgcaggcg cggcagacat attcgtaaat cggcatcggc ccctcgaact ggcaacgctg
     2281 gcgcagcatg cgtgctgcgc gcgcattttc aaggtttgcc ggccgcgaag ccagcgatca
     2341 ggttgcgctg cccggctcca gcaccggctc atccagcgcc ggccgcgcca cgctgggctc
     2401 ggcgggcagg atgatatgga actctgcacc gccttcgcgc ttcactgcgc ggatctcgcc
     2461 gccatgatgc gtcacgatct tgcggcagat ggccaggccg atgccggtgc cctcgaactc
     2521 atccgcgttg tgcagccggc ggaaaatcgc gaacacctgg tcgagctggt tggccggtat
     2581 gccaatgccg ttgtccgaca gcgagatgaa ccagccgctg ccgctgcgcg cgcacacgat
     2641 ctggatgcgc ggacggttgc cgggacgctg gaacttgatc gagttgccga tcaggttctg
     2701 gaacagctgg gtgagcaagg aatgctcggc cagtatttcc ggcagcgggt catggacgat
     2761 ttccgccccg ctcttctcga tctcgctggc cagtgctgcc ctggcgcggc cgatgctgta
     2821 gtccagcggg cgcacttcga aattgttgtc gctgcggccg acgcgcgaga gctgcagcag
     2881 gtcgcggatc agctgctgca tctgccggct gccctggtcg atgtagctga ggaattccag
     2941 cgcgtcgccg tcgagccgcg accggtagcg cgacgacagc agctgcgaaa agccggcgat
     3001 gttgcgcagc ggtgcctgca ggtcatgcga ggtgatgtag gtgaagctct ccagctcgcc
     3061 catggccgct ttcagttcgg ccaccgactg acgcaggttg gtttcggcga tgctgcgccg
     3121 cgcaacttca tcggacagct tgtggttggc ctcctccagt tcctcgggcg agcgcatgcg
     3181 cgccagcctg ggaaccagcg gaatgatggc gatggcggtg agtatcgaca cgatgccggt
     3241 agccgccttg accaggccct gcacgtagta gatcgggtgc cagacggtga tgatgtcgag
     3301 cacgtgggta atgccgcaca gcagaatgaa ggctgcgaac aggcccaccg cccagttgaa
     3361 gctgatcctc agcgtcttct t
//
LOCUS       DASZZC010000342         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_88671,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000342 DASZZC010000000
VERSION     DASZZC010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88671"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="hisS"
                     /locus_tag="VHE37_07230"
     CDS             complement(<1..873)
                     /gene="hisS"
                     /locus_tag="VHE37_07230"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HVT35356.1"
                     /translation="MSQRFQSLRGFNDILPADAEIWQRVEAIAREVFAAYGYGEIRLP
                     LLEHTGLFKRAIGEVTDVVEKEMFTFDDREGDSLTLRPEGTASCVRAGLEHGLLHNQQ
                     QRLWYAGPMFRHERPQAGRYRQFHQFGAEAYGMAGPDIDLEVIALSARLLKKLGLAEV
                     KLEVNSLGSSEARTAYRAALVAYLQQHESSLDADSKRRLHSNPLRVLDSKVPATQAIA
                     RDAPLLIDHLDTESRAHFDTLRQGLSDLGIAHVLNPRIVRGLDYYSRTVFEWTTTALG
                     AQGTVLAGGRYDGLV"
     gene            complement(870..1973)
                     /gene="ispG"
                     /locus_tag="VHE37_07235"
                     /gene_synonym="gcpE"
     CDS             complement(870..1973)
                     /gene="ispG"
                     /locus_tag="VHE37_07235"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVT35357.1"
                     /translation="MKSQHRITRRKSRQIRVGRVAIGGDAPIAVQTMTNTDTEDVAAT
                     VAQVRASMKAGADLVRISVPTLAAAAAFKQIKKEVGYVPLIADIHFNYKCALAAAEGG
                     ADCLRINPGNIGGERKVDEVVACARHHGVPIRIGVNAGSLEADLQEKYGEPNSDALVE
                     SAMRHIDMLVKRGFDDFKVSLKASEVFMTVEAYRKLAAQIEQPLHLGITEAGGLRSGA
                     VKSSIGLGMLLADGIGDTIRISLAADPVEEVKVGWDILKSLHLRHRGINLVACPSCSR
                     QNFDVVKVVNELESRLEDIDEPLDMAVIGCVVNGPGEAREAAIGVAGGYPNSIFVDGS
                     IQHKAHNDQLVDRLEKLVRERIAAQKAAKAAGA"
     gene            complement(2147..>3057)
                     /locus_tag="VHE37_07240"
     CDS             complement(2147..>3057)
                     /locus_tag="VHE37_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HVT35358.1"
                     /translation="AADGGLIPSPGQMIRNARERARLTMEDLAAQMKLARPTLDALEH
                     DNFEALLEPVYVRGYYRKCAKLLNLSEKDLIDAYQAMVSPKAPSAPSKLRLASGTELG
                     SDRKLPLWMAGGMGVLALIVASLVWNLVKAPPQPAPLLQPTPAITESAAPPVAPAAAT
                     TDTTGPGEEPARPAEPRAQNSAPTAVTTTTVAPAALPPKPAATAAAAPAAAGSSAASA
                     AGEEKPLTLEFSITSWARVSDGNGKTLLNSLMHAGDKRSFKAATPYAVFLGNAPGVTA
                     QFEGKSVDLAKYTGDNLTARFSLPEK"
BASE COUNT          452 a         1034 c         1032 g          539 t
ORIGIN      
        1 gaccaggccg tcgtagcggc cgcccgcgag caccgtgccc tgcgcgccca gcgcggtggt
       61 ggtccactcg aacaccgtgc ggctgtagta atcgaggccg cggacgatgc gcggattgag
      121 cacgtgggcg atgcccaggt ccgacagtcc ctggcgcagt gtgtcgaaat gcgcgcgcga
      181 ctccgtgtcg aggtgatcga tcagcagcgg cgcatccctg gcaatggcct gcgtggcggg
      241 caccttggaa tcgagcacgc gcagcggatt gctgtgcagg cgacgcttcg aatcggcatc
      301 gagtgagctt tcgtgctgct gcaggtacgc gaccaaggcc gcgcggtagg cggtacgcgc
      361 ctcgctgctg cccagcgaat tcacctccag cttcacctcg gccaggccca gcttcttcag
      421 cagacgcgcc gacagcgcaa tcacttccag gtcgatgtcc ggcccggcca tgccgtaagc
      481 ctcggcgccg aactggtgga actggcggta gcgcccggcc tgcggccgct cgtggcggaa
      541 catcgggccg gcgtaccaca ggcgctgctg ctggttgtgc agcaggccat gctccagccc
      601 ggcgcgcacg cacgaggcgg tgccttccgg ccgcagcgtg agcgaatcgc cctcgcggtc
      661 gtcgaaggtg aacatctcct tctccaccac gtcggtcact tcgccgatgg cgcgcttgaa
      721 cagcccggtg tgttcgagca gcggcaggcg gatctcgccg tagccgtagg cggcgaacac
      781 ctcgcgcgcg atggcttcca cgcgctgcca gatctcggcg tcggccggca ggatgtcgtt
      841 gaagccgcgc aggctctgga agcgctggct catgcgccgg ccgccttcgc cgccttctgc
      901 gccgcgatgc gctcacgcac cagtttctcg aggcgatcga ccagctggtc gttgtgcgcc
      961 ttgtgctgga tgctgccgtc gacaaagatc gaattcggat agccgccggc cacgccgatg
     1021 gccgcctcgc gcgcttcgcc ggggccgttg accacgcagc cgatcaccgc catgtccagc
     1081 ggctcgtcga tatcttccag gcgcgattcc agctcgttca ccaccttcac cacgtcgaag
     1141 ttctgccgcg agcagctcgg gcaggcgacc aggttgatgc cgcggtggcg caggtgcagc
     1201 gacttcaaga tgtcccagcc gaccttcact tcctccaccg gatcggcggc cagcgagatg
     1261 cggatggtgt cgccgatgcc gtcggccagc agcatgccca ggccgatcga cgacttcacc
     1321 gcgccgctgc gcaaaccgcc ggcctcggtg atgcccaggt gcagcggctg ctcgatctgc
     1381 gcggcgagct tgcggtaggc ctccaccgtc atgaacactt cgctggcctt gagcgagacc
     1441 ttgaagtcgt cgaagccgcg cttcaccagc atgtcgatgt ggcgcatggc ggattccacc
     1501 agcgcgtcgg aattcggctc accgtatttt tcctgcaggt ccgcctcgag cgaaccggcg
     1561 ttgacgccga tgcggatcgg cacgccgtgg tggcgcgcgc aggccaccac ttcatccacc
     1621 ttgcgctctc cgccgatgtt gcccgggttg atgcgcaaac agtcggcgcc accctcggct
     1681 gcggccagcg cgcacttgta gttgaagtgg atatcggcga tcagcggcac gtagccgact
     1741 tccttcttga tctgcttgaa cgcggcggcg gcggcgagcg tgggcaccga gatgcgcacc
     1801 aggtcggcgc cggccttcat cgatgcgcgc acctgcgcca cggtggcggc gacgtcctcg
     1861 gtgtcggtgt tggtcatcgt ctgcacggct atcggtgcat cgccgccgat ggccacgcgg
     1921 cccacgcgga tctgccgcga cttgcggcgc gtgatccggt gctgtgattt catggccatg
     1981 agagtgtccg tgtccgatgc cgcgtttcga ttgcgccgcc ggcaaaaagt gccgcgcggc
     2041 gcctccaccg catcggcatt ttgcctgtta taaaatatcg tttaatttaa actataaaca
     2101 ataatatata acagctcaac cggccatgcg gtgacggctt cagcgcctat ttttccggca
     2161 gcgagaagcg ggcggtgagg ttgtcgccgg tgtacttggc caggtcgacg ctcttgcctt
     2221 cgaactgagc ggtgacgccc ggcgcgttgc ccaggaacac cgcgtacggc gtggccgcct
     2281 tgaagctgcg cttgtcgccg gcgtgcatca ggctgttgag cagggttttg ccgttgccgt
     2341 cggagacgcg cgcccacgag gtgatggaga actccagcgt cagcggcttt tcctcgccgg
     2401 cggcggacgc ggcgctgcta cccgcggcag ccggcgcagc agcagcggtt gcagcgggct
     2461 tcggcggcag cgccgccgga gcgacggtcg tcgtcgtgac cgccgtcggc gcactgttct
     2521 gcgcgcgcgg ctccgcaggc ctggcaggtt cttcaccggg gccggtcgta tccgtggtgg
     2581 cggccgcggg cgccaccggc ggcgcggcgc tctcggtgat cgccggcgtc ggctgcagca
     2641 gcggcgcggg ctgcggcggc gccttcacca ggttccacac cagcgaggcc acgatcagcg
     2701 ccagcacgcc catgccgccg gccatccaca gcggcagctt gcggtcggaa cccagctcgg
     2761 tgcccgaggc caggcggagt ttggacggcg cgctcggcgc cttgggcgac accatcgcct
     2821 ggtaggcgtc gatcagatcc ttctcggaga ggttcagcag cttggcgcac ttgcggtaat
     2881 agccgcgcac gtacaccggc tcaagcaggg cctcgaagtt gtcatgctcc agcgcatcga
     2941 gcgtgggccg cgccagcttc atctgtgcgg cgaggtcttc catggtcagg cgcgcacgct
     3001 cgcgcgcgtt gcggatcatc tggccagggc tgggaataag gccgccgtcg gccgcag
//
LOCUS       DASZZC010000343         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_88757,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000343 DASZZC010000000
VERSION     DASZZC010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88757"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VHE37_07245"
     CDS             <1..699
                     /locus_tag="VHE37_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVT35359.1"
                     /translation="DQAGLKSGDLLTAANGQSLSAKSGDTPGPERKLLDVMAGLKPGD
                     TVKLDYERDGRNASTTATATRAQDSMLARRIVIPDLGPLVDFEDDDDHDIIVPPMPPM
                     PPQAPGAPAAPMPPLAHIHVWNDDSWDLAGIDTDLAPYFKTDAGVLVVKPARGSKLGL
                     KGGDVVQKVNGEAVKSPDELRRKLHQNAGKEVALDIVRQGKTQSVKGTLPKHEVTRMI
                     KKRIEINDGEDDPS"
     gene            877..1896
                     /locus_tag="VHE37_07250"
     CDS             877..1896
                     /locus_tag="VHE37_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVT35360.1"
                     /translation="MRAMFIERPGGHGVFAARDLPEPVPAPREVVIDVRAIGINFADV
                     LARQGIYPDAPPYPCCVGYEVSGVVCAAGREVDARWLGREVLALTDFNSYAQRVAVGV
                     DYVWDKPASLPFEQAAAIPLNYLTAWGLLVAMGGLARGDRVLIHNAGGGVGLAALDIA
                     RQLGAISIGTASARKHEFLRGRGLDHAVDYTRSDWPAQVRAASGGAGVELAIDPIGGA
                     SWKHTYALLAPTGRMGMFGISGAATASGLRGKWELAKLMLGAPLFHPARIIPANRGVY
                     GINIHAMYQRHDKFSSWMQAILRGVAEGWVRPHVDRVFALEQAGEAHRHIEARGNIGK
                     VLLVP"
     gene            1945..>2300
                     /locus_tag="VHE37_07255"
     CDS             1945..>2300
                     /locus_tag="VHE37_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVT35361.1"
                     /translation="MELCELGAAELARRIAAGELRSGEVVEAHIARIEAVNPKLNAVI
                     ARRYDDARREAKAADELRARGVVLPPLHGVPVTIKDSIDVAGLASTFGIPTLRNNHAK
                     NDEVHVARLRAAGAIVL"
BASE COUNT          407 a          770 c          802 g          321 t
ORIGIN      
        1 gaccaggccg ggctcaagtc cggcgacctg ctcaccgccg ccaacggcca gtcgctgtcg
       61 gcgaaatcgg gcgacacgcc ggggcccgag cgcaagctgc tcgacgtgat ggccggcctc
      121 aagcccggtg acacggtgaa gctggactac gagcgcgacg gcagaaatgc cagcaccacc
      181 gccaccgcca cgcgcgcgca ggattcgatg ctggcgcgtc gcatcgtcat ccccgacctc
      241 gggccgctgg tcgatttcga ggacgacgac gaccacgaca tcatcgttcc ccccatgcca
      301 ccgatgccgc cgcaggcgcc cggcgcgccg gctgccccga tgccgccgct ggcgcatatc
      361 catgtgtgga acgacgacag ctgggacctg gccggcatcg acaccgacct cgcgccgtac
      421 ttcaagaccg acgccggcgt gctggtggtg aaacccgcgc gcggcagcaa gctcggactg
      481 aaaggcggcg acgtggtgca gaaggtcaac ggcgaagcgg tgaagtcgcc cgacgaactg
      541 cgccgcaagc tgcaccagaa tgcgggcaag gaggtggcgc tggacatcgt gcgccagggc
      601 aagacgcaga gcgtgaaagg cacgctgccg aagcacgagg tcacccgcat gatcaagaag
      661 cgcatcgaga tcaacgacgg cgaagacgac ccttcctgat cgttcctggc cggtaaaacg
      721 gggtcaccgc atccgcccgg atgatgttat taaaaattgt attaaattaa aaataaacaa
      781 taattaataa cacacaaacc ggtggtgcgg tggcaggttt ttcgcccgct gccggtgctc
      841 ggctacgata gcccgcacaa aaaacgggag cggcgcatgc gggcaatgtt catcgagcgg
      901 ccgggcggcc atggcgtgtt cgccgcgcgt gatctgcccg agccggtgcc cgcgccgcgc
      961 gaagtggtga tcgacgtgcg cgccatcggc atcaacttcg ccgacgtgct cgcgcgccag
     1021 ggcatctacc cggacgcgcc gccgtatccg tgctgcgtcg gctacgaggt gtccggcgtc
     1081 gtatgcgcgg ccggccgcga ggtggacgcg cgctggctcg gccgcgaagt gctggcgctc
     1141 accgatttca attcctacgc gcagcgtgtg gcggtgggcg tcgattacgt gtgggacaaa
     1201 ccggcgtcgc tgccgttcga gcaggccgcg gccattccgc tgaactacct caccgcctgg
     1261 ggcctgctgg tggccatggg cggactggcg cgcggcgatc gcgtgctgat ccacaacgcc
     1321 ggcggcggcg tggggctggc ggcgctggac atcgcgcgcc agctgggcgc catcagcatc
     1381 ggcaccgcca gcgcgcgcaa gcacgagttc ctgcgcgggc gcgggctcga tcacgcagtc
     1441 gactacacgc gcagcgactg gccggcacag gtgcgcgcag ccagcggcgg cgccggcgtg
     1501 gaactggcca tcgacccgat cggcggcgcc agctggaagc acacctacgc actgctcgcg
     1561 cccaccgggc gcatgggcat gttcggcatc tccggcgccg ccacggcatc gggcctgcgc
     1621 ggaaaatggg agctggccaa gctgatgctc ggtgcgccgc tgttccaccc cgcacgcatc
     1681 atcccggcca accgcggcgt ctacggcatc aacatccacg ccatgtacca gcggcacgac
     1741 aagttctcgt catggatgca ggccatcctg cgcggcgtgg cggaaggctg ggtgcggccg
     1801 catgtcgacc gcgtgtttgc actggagcag gcgggcgagg cgcaccggca catcgaggcg
     1861 cgcggcaaca tcggcaaggt gctgctggtg ccgtgagtgc ggtggcgcgc cgcctcaccc
     1921 ggcaactcat ttggggagtt gaaaatggaa ctctgtgaac tgggcgcggc cgagctggcc
     1981 aggcgcatcg ccgccggcga gctgcgcagc ggcgaggtag tcgaggcgca tatcgcgcgc
     2041 atcgaggcgg tgaatccgaa gctgaacgcg gtgatcgcgc ggcgctacga cgacgcgcgc
     2101 cgcgaggcga aagcagccga cgagttgcgc gcgcgcggcg tggtgctgcc gccgctgcac
     2161 ggcgtgccgg tgacgatcaa ggactcgatc gacgtggccg gcctggcgtc caccttcggc
     2221 atacccacgc tgcgcaacaa ccacgcgaag aacgacgagg tgcacgtcgc gcggctgcgc
     2281 gcggctggcg ccatcgtgct
//
LOCUS       DASZZC010000344         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_931254,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000344 DASZZC010000000
VERSION     DASZZC010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931254"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2542)
                     /locus_tag="VHE37_07260"
     CDS             complement(<1..2542)
                     /locus_tag="VHE37_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT35362.1"
                     /translation="MLARMPLLRDAPIRHKLTVVSTLITSIALLFACIGFISYEQIAF
                     RTNLVEVLTTTAAMLGDNSSAALSFHDPASAEETLKSLHLDSNVIGAAIYDKDGHLFA
                     SYRGNSSSATPFMPPPSPGEGHRIGNGTVEVFAPIELAGDRAGTVYLASDMHELHRRL
                     QQYAATAATLMAIAAVAAFALSSRLLLPVTRPIVHLADVMDQVAANKDYSLRVVGQGR
                     DETGRLMQGFNDMLRQIQARDSALQHARDNLEEDVARRTSELQGAYAQLERELTEREQ
                     IEASLRASEERFRLSFESSAVGMAIVSLEGRWLGVNPALCQMLGYSEAELQATDFQSV
                     THPEDLAADLGHMQRLLAGEIPSYQMEKRYLHKSGRVVWIRLSAALVRSAGGDAQHFV
                     SQVEDITASKRAESLVTASELRYRRLFEAATDGILIVSADNGRILDVNPFLIDLLGYR
                     NDEWVGRCFREIEPLQGDNGIDFDELQKMPRLRRDDLQLVARDGRLVDVEFLSNTYFA
                     AEQKVVQCNLRDITQRKRVERALGAAEVRYRALIENVSDVIALTRDDGRLAYVSPSVK
                     LVVGYEPEELLDRNFFDLLHPDDIERATQIMQQITGSPGTAVRAELRYQRRDGSWVAL
                     ESMSKMLPPSAEVTGMAVALRDVSERRRAEQALAAQAADLKRSNAELEQFAYVASHDL
                     QEPLRAVAGSVQLLQRRYAGSLDARADEFIAHAVDGAKRMQQLINDLLDFSRVGTRGK
                     AFAEMPAERALQLALANLAVALRESGAVVTHGPLPVIVGDLSQITQLLQNLVGNAIKF
                     CRSRTPHIQVSAAREAAAWHFSVRDNGIGIEAEHLERIFVIFQRLHTRN"
     gene            complement(2542..3120)
                     /locus_tag="VHE37_07265"
     CDS             complement(2542..3120)
                     /locus_tag="VHE37_07265"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVT35363.1"
                     /translation="MKHNGPCGTLRRRALRSLALLAFWLPQATMLQAADTASREYQIK
                     AAFLYNFTKFVEWPASSFASDNSPIIIGTYCADPFADELAAIVAGRKVYNRPIAVRKL
                     GSGEEARSAHLVFVCADNDARVGSIESAVGRQPVLTVGESDTFDAAGGIIRFVAENDK
                     IRFEINADAAAHAGIRISAQLQKLARAVREQP"
     gene            complement(3117..>3952)
                     /locus_tag="VHE37_07270"
     CDS             complement(3117..>3952)
                     /locus_tag="VHE37_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35364.1"
                     /translation="LVLTGGLKLEHNDFTHLELQPSVRAIFAPAARQRVWAAVSRAVR
                     TPDVVEGRRAVDIVDGMPVSGPGGGMYLPTLVGNPDLKSSVLWAYEAGWRVQLSPSLS
                     ADLAGFFNHYEGVIGRGEITRYDAGSPVGIAEMPWGNNVNGETQGGELSLNAVPSPQW
                     RLTASYALLLAQLRGGSDASLLEHGSPRHQAILRSSWDAFDDGGLDTLWRYVSAIPGV
                     GAYTTADARLWYRPRPWLELSLVGKNLLDPQHAEQGTELLAINSEAPRTALLRVEMRF
                     Q"
BASE COUNT          618 a         1325 c         1333 g          676 t
ORIGIN      
        1 cgttgcgcgt gtgcaggcgc tggaagatga cgaagatgcg ctccagatgc tcggcctcga
       61 tgccgatgcc gttgtcgcgc acgctgaagt gccaggccgc ggcctcgcgc gcggcactca
      121 cctggatgtg cggcgtgcgg ctgcggcaga acttgatggc attgcccacc aggttctgca
      181 gcagttgggt gatctgcgac aggtcgccga cgatcaccgg cagcggtcca tgcgtgacca
      241 cggcgccgct ctcgcgcagc gctaccgcaa ggttggccaa tgccagctgc agggcacgtt
      301 cggccggcat ctcggcaaag gccttgccgc gcgtgccgac ccgggagaag tcgagcaggt
      361 cgttgatcag ctgctgcatg cgcttggctc catcgacggc gtgggcgatg aactcatcgg
      421 cgcgggcgtc gaggctgccg gcatagcggc gctgcagcaa ctgcacgctg cccgccacgg
      481 cacgcagggg ctcctgcagg tcatgcgagg ccacgtaggc aaactgctcg agctccgcgt
      541 tcgagcgctt gagatcggcg gcctgcgccg ccagcgcctg ttcggcacgc ctgcgctcgc
      601 tgacatcgcg cagcgccacc gccatgccgg tgacctcggc agagggcggc agcatctttg
      661 acatcgactc cagcgccacc cacgaaccgt cgcggcgctg gtagcgcagc tcggcgcgta
      721 cggcggtgcc gggcgagccg gtgatctgct gcatgatctg cgtggcgcgc tcgatgtcgt
      781 cgggatgcag caggtcgaag aaattccggt cgagcagttc ctcgggctcg tacccgacca
      841 ccagtttcac gctcgggctg acgtaggcca gccgtccgtc gtcgcgggtc agcgcgatca
      901 cgtcggacac gttctcgatc agcgcgcggt aacgcacttc cgcagcgccg agcgcgcgtt
      961 ccacgcgctt gcgctgggtg atgtcgcgca ggttgcactg cacgactttc tgctcggccg
     1021 cgaaataggt gttgctgagg aactcgacgt cgaccaggcg cccgtcgcgc gcgaccagtt
     1081 gcaggtcgtc gcgccgcagc cgcggcattt tctgcaattc gtcgaagtcg atgccgttgt
     1141 cgccttgcag gggctcgatc tcccggaagc accgccccac ccattcgtcg ttgcggtagc
     1201 cgagcaggtc gatgaggaac ggattgacat cgaggatgcg gccattgtcg gcgctgacga
     1261 tgagtatgcc gtcggtcgct gcctcgaaca ggcgccggta gcgcagttcg gacgccgtca
     1321 ccaggctttc ggcgcgcttg ctggcggtga tgtcttccac ctggctgacg aaatgctgtg
     1381 catcgccgcc ggcgctgcgg accagcgccg cgctcaggcg gatccacacc acgcggccgc
     1441 tcttgtgcag gtagcgcttt tccatctggt acgacggaat ctcgcctgcc agcaggcgct
     1501 gcatgtgccc caggtccgcc gccaggtctt ccgggtgggt gacgctctgg aagtcggtcg
     1561 cctgcagttc ggcctcgctg tagccgagca tctggcacag cgcggggttg acgcccagcc
     1621 agcgcccttc gagcgagacg atggccatgc ccacggccga cgactcgaag gacaggcgga
     1681 aacgttcttc gctggcgcgc agtgacgctt cgatctgttc gcgctcggtc agctcccgct
     1741 ccagttgcgc gtaggcgccc tgcagctccg aggtgcggcg ggcgacatcc tcctcgaggt
     1801 tatcccgcgc gtgctgcagc gcgctgtcgc gcgcctggat ctgccgcagc atgtcgttga
     1861 acccctgcat cagccgcccg gtctcgtcgc gcccctgccc gaccacgcgc agcgagtagt
     1921 ccttgttcgc ggccacctgg tccatcacgt ccgccaggtg cacgatcggc cgcgttaccg
     1981 gcagcagcag gcgtgacgac agggcgaatg ccgcgaccgc cgcaatggcc atcaatgtgg
     2041 cggcggtcgc ggcatattgc tgcaggcggc ggtgcagctc gtgcatgtcg ctggccaggt
     2101 agacagtgcc ggcgcggtcc ccggccagtt cgatcggcgc gaacacctcc accgtgccgt
     2161 tgccgatgcg atggccctcg cccggcgacg gcggcggcat gaaaggcgtc gccgaggacg
     2221 aattgccgcg atagctggcg aacaggtggc cgtctttgtc atagatcgcc gcaccgatca
     2281 cgttggaatc caggtgcagc gacttcagcg tttcttccgc cgaagcggga tcgtggaatg
     2341 acagcgccgc ggaactgttg tcgcccagca tggccgccgt ggtggtgagc acttccacca
     2401 ggttggtgcg gaacgcgatc tgttcgtagc tgatgaagcc gatgcaggca aacagcagcg
     2461 cgatgctggt gatcagcgtg ctgaccacgg tcagcttgtg gcggatgggg gcatcacgta
     2521 gcagcggcat gcgcgccagc atcagggctg ctcccgcact gcccgcgcca gtttctgcag
     2581 ctgcgcgctg atacggatgc cggcatgcgc cgcggcatcg gcattgatct cgaagcggat
     2641 cttgtcgttc tccgccacga aacggatgat cccacctgcc gcgtcaaagg tgtcggattc
     2701 gccgaccgtg agcacgggct ggcgccccac tgcgctttcg atgctgccga cccgcgcatc
     2761 gttgtcggca cagacgaata ccaggtgcgc gctgcgcgcc tcctcgccgc tgcccagctt
     2821 gcgcacggca atgggccggt tatacacctt gcgcccggcg acgatggcgg caagctcgtc
     2881 ggcaaacgga tccgcgcagt aggtgccgat gatgatgggg ctgttgtccg aggcgaaact
     2941 gctggccggc cactcgacga atttggtgaa gttgtagagg aacgctgcct tgatctggta
     3001 ttcgcgtgat gccgtatccg ccgcctgcag catcgtggcc tgcggcagcc agaaagccag
     3061 caacgcgagc gagcgcaacg cacgccggcg cagggttccg cacggaccgt tgtgcttcat
     3121 tggaagcgca tctccacgcg cagcagcgcg gttcgcggag cttcggagtt gatcgccagc
     3181 agctccgtgc cctgctcggc atgctgcggg tccagcaggt tcttgccgac caggcttagc
     3241 tcgagccatg gccgcggccg gtaccacagg cgggcgtccg cggtggtgta tgcgccgaca
     3301 cccgggatcg cgctgacgta gcgccacagc gtgtcgaggc cgccgtcgtc gaacgcatcc
     3361 caggacgagc gcaggatcgc ctgatggcgc ggtgatccat gctcgagcaa ggaagcgtcg
     3421 ctgccgccgc gcagctgcgc cagcagcagt gcgtaggacg ccgtcagccg ccactgcggc
     3481 gacggcacgg cgttcaggga caactctccg ccctgggtct cgccgttgac gttgttcccc
     3541 cagggcatct cggcgatgcc caccgggctg cccgcgtcgt agcgggtgat ctcacccctg
     3601 ccgatcacgc cttcgtaatg gttgaagaag ccggcaagat cggcgctgag cgacggcgac
     3661 agctgcacgc gccagccagc ctcgtaagcc cacagcacgg acgatttcag atcggggttg
     3721 cccaccagcg tgggcaggta cataccgccg ccagggccgc tgaccggcat gccgtcgacg
     3781 atatcgaccg ctctgcggcc ctctaccaca tcgggcgtgc gcacggcacg cgacaccgcc
     3841 gcccatacgc gctgccgtgc agccggcgca aagatggcgc gcacgctcgg ctgcagctcc
     3901 aggtgcgtga agtcgttgtg ctcgagcttg aggccgccgg tgagcaccag cc
//
LOCUS       DASZZC010000345         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_931263,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000345 DASZZC010000000
VERSION     DASZZC010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931263"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VHE37_07275"
     CDS             complement(<1..1485)
                     /locus_tag="VHE37_07275"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HVT35365.1"
                     /translation="MSMDKTYEPAAIEARWRERWEADGSYAPSGNGDPYSIMIPPPNV
                     TGTLHMGHAFQHTIMDALTRHARMSGKDTLWQPGTDHAGIATQMVVERNLKLAGEPSR
                     AELGREKFLEKVWEWKKYSAGTIGQQIRRMGSSVDWQSEAFTMSPEYSKAVIEHFVKL
                     HEDGLIYRGKRLVNWDPVLQTAISDLEVVSEEENGKLWHIRYPLADGSGHLVVATTRP
                     ETMLGDAAVAVHPEDERYQKLIGKEVELPLCNRRIKILADEFVDREFGTGVVKITPAH
                     DFNDYAVGQRHGLPLINIFDKSGRIVGDLANSVQQPQSLRETISQAADAAESRELIPV
                     KYRGMDRFLARKEIVRDLEAAGLMEKIEDHKLKVPRGDRSGAVLEPYLTDQWFVDLTR
                     KDGGWKKITQPAIDAVESGRVRFVPDNWKTTYFHWLNNIQDWCISRQLWWGHRIPAWY
                     DAQGKVYVGRSEDEVRAKNNLSPAETLRQDEDVFDTWFSSDIWPF"
     gene            complement(1585..2055)
                     /locus_tag="VHE37_07280"
     CDS             complement(1585..2055)
                     /locus_tag="VHE37_07280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HVT35366.1"
                     /translation="MTRIDFYILPEQASAAEGAVMTACKLCDKATGAGMKVYIHAPDA
                     ALAEEIDGALWSFRQGSFIAHEKYLGRPLEAPLPAALIGEIEPPDTHHGAMINLSPSV
                     PSFFSRFERVLEIVPGDADSRAKCRERYKFYRDRGYELRTYEQNSEGGWKLRAA"
     gene            complement(2072..>3513)
                     /locus_tag="VHE37_07285"
     CDS             complement(2072..>3513)
                     /locus_tag="VHE37_07285"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HVT35367.1"
                     /translation="RRTPSEAAATLDQVSNGVIGSVMRRGDMDGKLGQSLMLHSVPNI
                     YADRVLLVGLGKERSFDDAAVRRAHATAARALRGTGSIDAISYLTQIVPKNRDFQWSV
                     QQAVLATEDSYYRFDDCRGDKAKQDKSSVKLERYTFDVPRRSDLPAGEQGLAEGLAIA
                     HGVNLAKNLGNLPGNICTPTYLAQAAQKLAEKSPIKVKVLERAEMEKLGMGALLSVAK
                     GSSQPPKLIVMEYSNGAKGDKPIALVGKGLTFDAGGISIKPADKMDEMKFDMCGGAAV
                     IGAMAAAAELKLKLNLVGVVPATENLINGEANKPGDIVTSMAGLTIEVLNTDAEGRLI
                     LCDALTYVEKNHDPAVCIDMATLTGACVVALGSVATGLFANSNSLGRALLAAGEQIGD
                     RAWELPLWDEYDGNIKSEFADMANIATRGGREAGAIIGAIFLQRFARKMKWAHLDIAG
                     TAWSGKRSTGRPVPMLVQYLINATGKKDA"
BASE COUNT          487 a         1151 c         1166 g          709 t
ORIGIN      
        1 gaacggccag atgtcggagg agaaccaggt gtcgaacacg tcctcgtcct gcctgagcgt
       61 ctcggcggga ctgagattgt ttttggcgcg aacttcgtct tcgctgcggc cgacgtacac
      121 cttgccctgc gcgtcgtacc acgcggggat gcgatgaccc caccacagct ggcggctgat
      181 gcaccagtcc tggatgttgt tgagccagtg gaagtacgtc gtcttccagt tgtccggcac
      241 gaagcgcacg cggcccgact cgacggcgtc gatggccggc tgcgtgatct tcttccagcc
      301 gccgtctttc ctcgtcaggt caacaaacca ctggtcggtg aggtacggct ccagcaccgc
      361 gccgctgcgg tcgccgcgcg gcaccttgag cttgtgatcc tcaatcttct ccatcaggcc
      421 ggcggcttcc agatcgcgca cgatctcctt gcgagcaagg aaacggtcca tgccgcggta
      481 tttgacgggg atcagctcgc gactttcggc agcgtccgca gcttgcgaaa tggtttcgcg
      541 aagtgactgt ggttgctgaa ctgaattggc aaggtctcct acgatcctgc cgctcttgtc
      601 gaagatattg atcagcggca aaccatgccg ctgccccacc gcgtagtcgt tgaagtcgtg
      661 cgccggcgtg atcttcacca cgccggtgcc gaattcgcgg tcgacgaatt cgtcggccag
      721 gatttttatc cggcggttgc acagcggcag ctcgacttcc ttgccgatca atttctggta
      781 acgctcgtct tccgggtgca ccgccaccgc ggcgtcgccg agcatggttt ccggtcgcgt
      841 ggtcgccacc acgagatgcc cgctgccatc ggccagcggg tagcggatat gccagagctt
      901 gccgttctcc tcctcgctca ccacttccag gtcggagatc gcggtctgca gcaccggatc
      961 ccagttgacc aggcgcttgc cgcggtagat caggccatcc tcgtgcagct tcacgaaatg
     1021 ctcgatcacc gccttcgagt attccgggct catggtgaag gcttcgctct gccagtcgac
     1081 gctggagccc atgcgccgga tctgctggcc gatggtgccg gcggaatatt tcttccactc
     1141 ccagaccttc tccaggaatt tctcgcggcc gagttccgct cgcgagggct cgcccgcgag
     1201 cttgagattg cgctccacca ccatctgcgt ggcgatgccg gcgtggtcgg tgcccggctg
     1261 ccacagcgtg tccttgccgc tcatgcgcgc gtggcgcgtc agcgcgtcca tgatggtgtg
     1321 ctggaacgcg tggcccatgt gcagcgtgcc ggtgacgttg ggcggcggga tcatgatcga
     1381 gtagggatcg ccgttgccgg atggcgcgta actgccgtcg gcttcccagc gttcgcgcca
     1441 gcgcgcctcg atggcggcgg gttcgtaggt cttgtccatg ctcatctgtt tatcttgtca
     1501 ttcccgcgaa ggcgggaatc cagtttgggt caaagagtgg cgaggaaaac tggatgcccg
     1561 ccttcgcggg catgacggct gtggttacgc agcgcgcagc ttccatccac cttctgaatt
     1621 ctgctcgtag gttctcagtt catacccgcg gtcgcggtag aacttgtagc gctcgcggca
     1681 tttcgcgcgg ctgtcggcgt cgcccggcac gatctccagc acgcgctcga agcgcgagaa
     1741 gaagctcggc accgacggag acaggttgat catggcgccg tggtgcgtgt cgggcggttc
     1801 gatctcgccg atcagcgccg cgggcagcgg cgcttccagc gggcggccga gatatttctc
     1861 gtgcgcgatg aagctgccct ggcggaagct ccacagcgcg ccgtcgatct cctcggccag
     1921 cgccgcgtcc ggcgcgtgga tgtacacctt catgccggcg ccggtggcct tgtcgcacag
     1981 cttgcacgcg gtcatcaccg cgccttcggc cgcgctggcc tgctccggca ggatgtagaa
     2041 atcgatgcgc gtcatcgcgg gccgcggcgc ctcaggcgtc cttcttgccg gtggcgttga
     2101 tcaggtactg caccagcatc ggcaccggcc ggccggtgga gcgcttgccg ctccaggcgg
     2161 tgccggcgat gtccaggtgc gcccatttca tcttgcgggc gaagcgctgc aggaagatgg
     2221 cgccgatgat ggcgccggcc tcgcggccgc cgcgcgtggc gatgttggcc atgtcggcga
     2281 actcgctctt gatgttgccg tcgtactcgt cccacagcgg cagttcccag gcgcggtcgc
     2341 cgatctgctc gccggcggcc agcagcgcgc ggcccaggct gttgctgttg gcgaacaggc
     2401 cggtggccac gctgcccagc gccaccacgc aggcgccggt gagcgtggcc atgtcgatgc
     2461 acaccgccgg gtcgtggttc ttctcgacgt aggtcagcgc gtcgcacagg atcaggcggc
     2521 cctcggcgtc ggtgttgagc acctcgatgg tcaggccggc catgctggtg acgatgtcgc
     2581 cgggcttgtt ggcctcgccg ttgatcaggt tctcggtggc cggcaccacg ccgaccaggt
     2641 tgagcttgag cttgagctcg gccgccgccg ccatggcgcc gatcaccgcc gcgccgccgc
     2701 acatgtcgaa cttcatctcg tccatcttgt cggccggctt gatcgaaatg ccgccggcgt
     2761 cgaaggtcag gcccttgccg accagtgcga tcggcttgtc gcccttggcg ccgttgctgt
     2821 actccatgac gatgagcttg ggcggctggc tgctgccctt ggccaccgac agcagcgcac
     2881 ccatgccgag cttttccatt tcggcgcgtt ccagcacctt gaccttgatc ggcgattttt
     2941 ccgccagctt ctgcgcggcc tgcgccaggt aggtgggcgt gcagatgttg ccgggcaggt
     3001 tgcccaggtt cttggccagg ttcacgccgt gggcgatggc caggccctcg gccaggccct
     3061 gctcgccggc gggcaggtcg gaccggcgcg gcacgtcgaa ggtgtagcgc tcgagcttga
     3121 ccgacgactt gtcctgcttg gccttgtcgc cgcggcagtc gtcgaagcgg tagtaggaat
     3181 cctcggtggc cagcaccgcc tgctgcacgc tccactggaa gtcgcggttc ttcggcacga
     3241 tctgcgtcag gtagctgatg gcgtcgatcg agccggtgcc gcgcagtgcg cgtgcggcgg
     3301 tggcatgcgc gcggcgcacg gcggcgtcgt cgaagctgcg ttccttgccc aggccgacca
     3361 gcagcacgcg gtcggcgtag atgttcggca cgctgtgcag catcagcgat tggccgagct
     3421 tgccgtccat gtcgccccgg cgcatcacgc tgccgatcac gccgttgctg acctggtcca
     3481 gggtggcggc ggcctcgctg ggcgtgcggc ggt
//
LOCUS       DASZZC010000346         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_281876,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000346 DASZZC010000000
VERSION     DASZZC010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281876"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VHE37_07290"
     CDS             complement(<1..1295)
                     /locus_tag="VHE37_07290"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HVT35368.1"
                     /translation="MLDRFPLLKIRPGFALGLAAAALAPLAHADDAAAPAVPAAPAQQ
                     ESVTEELIRLLAEHNALSRDDAEKLIQRLKAEHPAPAATDAAAAPPEKGRVRVIYMPE
                     TERARIKEEVKQEVMQQARDENWAQPEAVPSWTKRLRFDGDIRFRQEFDMYGSGNAAA
                     RFPAYNFQAINSASTPPDTNPNNTQVFPFLDTTENRELPRIRARLGLTATIADDITGA
                     IRIATGNTTNPVSTNQTLGTDFNKASFVLDRAYLAFQPMQELQVSVGRMPNPWLSTEM
                     IWDEDLNFDGLAARYHWLGDLSPFVTLGAFSIENSALDFPSTQSAKFPSRDKWLFGGQ
                     LGANWRFAPESMWKGGVAFYDFYHLEGTFLPCFAPTAQVQCEADQSRSMFMQKGNTLF
                     GVRDVLPFATPSPTDPAYNYFGLASPFRVLDVTTSIDFAL"
     gene            complement(1356..>1586)
                     /locus_tag="VHE37_07295"
     CDS             complement(1356..>1586)
                     /locus_tag="VHE37_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT35369.1"
                     /translation="ESALRRQRQLSSTEYRLRVLVWITAEGAIENVKLVGSTGKPEID
                     RLVRESMATAERMAQPPPKDMPQPVQLQITSS"
BASE COUNT          238 a          488 c          561 g          299 t
ORIGIN      
        1 agcgcaaagt cgatgctggt ggtcacgtcc agcacacgga acggcgaagc cagtccgaag
       61 tagttgtagg cggggtccgt cgggctgggc gtggcgaacg gcagcacgtc gcgcacgccg
      121 aacagcgtat tgcccttctg catgaacatg gaccggctct ggtcggcttc gcactgcacc
      181 tgcgcggtcg gcgcgaagca cggcaggaac gtgccttcca ggtggtagaa gtcgtagaag
      241 gcaactccgc ctttccacat cgactcgggc gcgaagcgcc agttggcgcc gagctgcccg
      301 ccgaacagcc acttgtcgcg gctggggaac ttggcggact gcgtgctggg gaaatccagt
      361 gccgagttct cgatcgaaaa cgcgccgagg gtcacgaagg gcgagagatc gccgagccag
      421 tggtagcgcg ccgccaggcc atcgaagttc agatcctcat cccagatcat ctcggtcgac
      481 agccagggat tgggcatgcg gcccacgctg acctgaagtt cctgcatcgg ctggaaggcg
      541 agataggcgc ggtcgagcac gaagctggcc ttgttgaaat cggtgcctag cgtctggttg
      601 gtcgacaccg ggttggtggt gttgccggtg gcgatgcgga tcgcgccggt gatgtcgtcg
      661 gcgatcgtcg cggtcaggcc caggcgggcg cggatgcgcg gcagctcgcg gttctcggtg
      721 gtatcgagaa agggaaacac ctgagtgttg ttcgggttgg tatccggcgg cgtggacgcg
      781 ctgttgatcg cctggaagtt gtacgccggg aaccgcgctg cggcgttgcc cgagccgtac
      841 atgtcgaatt cctggcggaa gcggatgtcg ccatcgaagc gcagccgctt ggtccagctc
      901 ggcaccgctt cgggctgcgc ccagttctcg tcgcgcgcct gctgcatcac ttcctgcttc
      961 acttcctcct tgatgcgcgc gcgctcggtc tccggcatgt agatcacacg cacgcggcct
     1021 ttttccggcg gcgcggccgc ggcgtcggtg gcggctggtg ccggatgctc ggccttcagg
     1081 cgctggatca gcttctcggc atcgtcgcgc gacagcgcgt tgtgttcggc gagcaggcgg
     1141 atcagttctt cggtcacgct ctcctgctgc gcgggcgcgg cgggcacggc cggcgcggcg
     1201 gcgtcatcgg catgcgccag cggcgccagc gctgcggcgg ccagaccgag cgcaaacccg
     1261 gggcggattt tcaacagggg aaatctgtcc agcatggctg caagtgtccg aagttcgtgt
     1321 ttcaggttcg ttgcgctttc cgacaggaag cgggctcacg atgaagtgat ctgcagctgc
     1381 accggctgcg gcatgtcctt gggcggcggc tgtgccatcc gttcggcggt ggccatggat
     1441 tcgcgcacca gcctgtcgat ctccggcttg ccggtgctgc cgaccagctt cacgttttcg
     1501 atcgcgcctt ccgcggtgat ccacaccagc acgcgcagcc ggtattcggt ggagctgagc
     1561 tggcgctgcc ggcgcagcgc gctctc
//
LOCUS       DASZZC010000347        11161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_32320,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000347 DASZZC010000000
VERSION     DASZZC010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11161
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32320"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="VHE37_07300"
     CDS             <1..90
                     /locus_tag="VHE37_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT35370.1"
                     /translation="ARYNKTKAAQLLGMSFRSLRYRIKKLKIE"
     gene            281..877
                     /locus_tag="VHE37_07305"
     CDS             281..877
                     /locus_tag="VHE37_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVT35371.1"
                     /translation="MSNLAPVVFDGAPSPGRGARAHRGVAALRQAGASGSYDALAPML
                     RRLLRNACTVLGASQGFFVVSRSGMAYDIACAHNMRPSEVMDVALHDAAAPLSATLSE
                     RLVAAGDLCGETMPVDDGLFDAHSPAVLCVPLELGAQENGAMCLLRLQARQRISDLDL
                     EIIQAVAEQAALALTAARQTRALTRLQASLNSLPAACA"
     gene            908..1723
                     /locus_tag="VHE37_07310"
     CDS             908..1723
                     /locus_tag="VHE37_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVT35372.1"
                     /translation="MRKNLRFRNEDPEAFRQAVLRQVQELTAANERLVASVVKNGKRF
                     RSLARSVWHVQEEERRRLARELHDGLGQTLTALANQLQRIHDDAAAQSNLGLGHRLSD
                     ALDITRGALHDTRELSRLLRPTLLDDLGLEAALKWLGRTLGERAGLAIEVHSTLGDAR
                     MAPDLETLVYRITQEALTNVIKHSGAKQAQIVLTRAPQELKLLVHDDGRGFEPAAQAT
                     PEKGALAGGLRGMRDRAELFGGRVRLRSEPGEGTSVELALPIDGDDFLHGAGA"
     gene            1720..2373
                     /locus_tag="VHE37_07315"
     CDS             1720..2373
                     /locus_tag="VHE37_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT35373.1"
                     /translation="MSAANPIKLIVVDDHNIVREGLVSMLEETGECQVLAQAADGAEA
                     MELAAKHRPDVVVTDLSMPRLSGLELVRRIREKMPQVKTLVLTVHEEDEYIVPIIRAG
                     ASGFLNKDTSVGELMTAIKTIHAGQGYFGARAKEALAAQQARPREAGLNPYDTLTARE
                     RETFQLVVEGKTTKEIAQILSIGVKTAENHRARMMEKLALRNTAEVVRFAARKGLLP"
     gene            2421..2984
                     /locus_tag="VHE37_07320"
     CDS             2421..2984
                     /locus_tag="VHE37_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVT35374.1"
                     /translation="MRHLTLLILLSAFTTGAHAGVYKCTENGQTFFSDKPCASKEVRE
                     TGRMDDKPVLSIGAQNGAANASGGTHTSTSNVPLPGPIDFGQEPVERLNKAMAILNSV
                     QSDGQNCDAALKSAPNDARTTVLCKNFIAQLRDGAQWPQAVDQIKELVKDSRLVEDNY
                     DAFKQSRVINENINALWKTAKLGLHES"
     gene            3297..3896
                     /gene="petA"
                     /locus_tag="VHE37_07325"
     CDS             3297..3896
                     /gene="petA"
                     /locus_tag="VHE37_07325"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HVT35375.1"
                     /translation="MSNEGVDAKRRRFLTLATTVVGGAGVVGAAIPFLASWSPSERAK
                     TLGAPVEADIGQIELGQMITFVWRGQPVWVVHRTKEMLDGLSKVDGDLVDPESKAKQQ
                     PDYIKGEARAIKPEYMVLKGVCTHLGCSPKFRPDHPAPEIAPNWQGGFFCPCHGSKFD
                     LSGRVYKGVPAPLNLVVPPHYYKDDHTLVIGADSNKGAA"
     gene            3896..5146
                     /locus_tag="VHE37_07330"
     CDS             3896..5146
                     /locus_tag="VHE37_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HVT35376.1"
                     /translation="MAIVPNPHKTTGFLGWIDDRFPLTKLWKDHLAEYPAPKNFNVWY
                     VFGSLALLVLVNQLLTGIWLAMHYKPDANQAWDSVEYIMRDVPWGNVLRYLHSTGASA
                     FFVVVYLHMYRGLLYGSHRKPRELIWLFGCLIFLALMAEGFCGYVLPWGNMSYWGAQV
                     IINLFGTIPFVGPDLVVWIQGDYIPADATLNRLFSLHVVALPFVIVGLVVAHIIALHE
                     VGSNNPDGVEIKKKKDANGWPLDAVAFHPYYTVKDILGVVIFLIIFLGIVFFSPDGGG
                     YLLEKPNFTPANPLSTPEHITPAWYFGAFYAMLRATPSFAGTQIWGVIVMFAAVVVLF
                     FLPWLDRSPVKSIRYKGTLSKIAIAIFTVTFIMLNYLGMQKPSTTATLLAQIGTILYF
                     AFFAALPFIPQFEQTKPVPERTTP"
     gene            5143..5895
                     /locus_tag="VHE37_07335"
     CDS             5143..5895
                     /locus_tag="VHE37_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HVT35377.1"
                     /translation="MKGIFLGLAALTLSLGAHAEGEALPLQFKLQPDNMAAAQRGARD
                     FMAYCSGCHSMRNLRYSRLGRDLGIPDDKLKANLMFTSDKPGDQIKSAMPADDAKKWF
                     GQTPPDLTVEARMRGPEWLYNYLMTFYVDPTRPTGMNNLVLPNASMPHVLWELQGLQA
                     KKAEPRLGEGNAEAAAEHEGGTGLELVQPGSQTPEQYEKTVADIVTFMAYAAEPGRAQ
                     RIATGAWVMAYLIFLLLPLTYFLKKEYWKDVH"
     gene            5977..6609
                     /locus_tag="VHE37_07340"
     CDS             5977..6609
                     /locus_tag="VHE37_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVT35378.1"
                     /translation="MVTLTRSKYVMAEKPAARAGVTLFASKQSLSCLWVRLVLLEKDV
                     EGARVEEVLPGRPNHDLAILNPALTLPTLVDREVVLHPALVIVEYLDERYPHPKLLPS
                     DPVARARIRMTIQHIESQLYALVGDIRARGAGAKAARKQLAEQLTGAAGMFPQRGWFL
                     GQEYNLADCAWAILLHTLTQLDLQLPGNLQAMVRYAERLFARPAVQKLLR"
     gene            6606..7013
                     /locus_tag="VHE37_07345"
     CDS             6606..7013
                     /locus_tag="VHE37_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HVT35379.1"
                     /translation="MSTSRRPYIIRAIYEWAVDNGYTPHLLVAADYPGVSVPREHVRE
                     GRITLNVSPMAVQSLDLKADPIWFSARFSGHAYDVLVPPGAVLAVFARENGEGFMFGE
                     IEPPDPAKAAAGDTPQTPPTKPAGGKPKLRIVK"
     gene            complement(7021..8076)
                     /locus_tag="VHE37_07350"
     CDS             complement(7021..8076)
                     /locus_tag="VHE37_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35380.1"
                     /translation="MGFQGKGEPFYPTVFQRIFADIEFDRGEVFLVGAGMLGKLLCAR
                     IRDLGGIAMDVGSIMDHWAGFATRAESAHQYTRFDPAASLLAGQPFAAMLPGCDESPA
                     PRCASDDTRRRNIAAELESLDCPLPTASHHFRVVAVGHPRCGSAYLTEVLRRLGLHIG
                     HEHCGDDGICSWPWTVDDARPPYGTPPPAAAAARVDCLLAYVRDPAQAIPSIMLENTD
                     DASFSFRRFHILRTSGMDIASYRTAMDRAVASYLGWMRIVDMRKPLAVIRIEHATEDL
                     RAIAAALPPPPSADGSAAWADLADVPRTINSSKEKFAAGKPQLSAAQIASLDRDLARE
                     LDEFCARHHYAQWATSR"
     gene            complement(8234..9625)
                     /locus_tag="VHE37_07355"
     CDS             complement(8234..9625)
                     /locus_tag="VHE37_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HVT35381.1"
                     /translation="MPEPLPSAFLQRCAEILPRERLYTDPADCLAYGYDNSKRMLLPQ
                     AVAFALTHDEAAAVVAACNEFSIPLIARGRATNTTGATVPVAGGVVLSLERMNRVLRV
                     SPGDRMMECEAGVINTDAQAVAEKHGLFWGPDPTSAGYSTIGGNLACNAGGPRTVKYG
                     TARDNVLGLRAIGGTGASLKTGCYTTKGVVGYDLTRLIVGSEGTLAIITEATLKLLPK
                     PPVTRTLRACYVDVNSAAAAVARVMQQPETPSALEFMDGDAVMLAQQFQDTGVPAGTG
                     ALLVIEVDGSREAIDHAVRAVGEAANGDGLVELKMAGSDAETAALWACRKALPPSLRK
                     IAPKKVNEDVAVPVTRLPELIAGLTQLSRQHGLRIVNFGHAGNGNLHVNILADPEDPA
                     QMKVIEPCLHGVFRLVLSLEGTISGEHGVGIEKRDYVGWEIAADTLETMRQLKRAMDP
                     RGILNPGKCLPGI"
     gene            complement(9618..9956)
                     /locus_tag="VHE37_07360"
     CDS             complement(9618..9956)
                     /locus_tag="VHE37_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HVT35382.1"
                     /translation="MKGAQEENLALAYLQAQKMQLVERNYRCRGGELDLVMRERDMLV
                     IVEVRKRGNPNFGSAAESVTGRKQARIVHAAQSFLASHSEFAHCPVRFDVIALDAANR
                     IEWLPGAFDA"
     gene            complement(9953..>11161)
                     /locus_tag="VHE37_07365"
     CDS             complement(9953..>11161)
                     /locus_tag="VHE37_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HVT35383.1"
                     /translation="EHGDVESWRTRYPGHPGAERIASIAEHPVVAPAAAAAAPASVPL
                     PQAGPKGLVALLLPLTGPTAASGEAVRDGFLSARFAAGASAAPVRIYDSGSAPAASVA
                     AYQQALHDGASLVVGPLRKESVVAIANVGVLSTPVLALNYLEPAASPSNFYQFGLAPE
                     DEARAAAENAIGRDLRSAVALVPKADWGERALAAFSQRFTELGGHVLKAARYDPSLRD
                     FTVPIQELLSVDASNERHRAITSLLGKKTEFEPHRRDDIDVVFLAARPSEGRLIWPQL
                     RYNGAGDLAAYATSLIYSGTAEPDLAGLAFCDMPWMLGGAAPLENLHAETTEVPGARF
                     QPRLFAMGYDAYALAGVIASGQLQHGASFGAATGELALGSNGVIARRLQCAQIDSGKL
                     TPLAASGSGL"
BASE COUNT         1962 a         3741 c         3623 g         1835 t
ORIGIN      
        1 gcgcgctaca acaagaccaa ggccgcgcag ctgctgggca tgtcgttccg ttcgctgcgc
       61 taccgcatca agaaactgaa aatcgaatag cgcggatgcg cttgggagaa aacatcgcat
      121 cctgcccgca gccgcggtga cctccctcca ttgatgtatg atgcaccgcc gatctgaacc
      181 ggatgtcagg tccgggtcgg cacaaacgac gaaaggcacg ttagttgcct agccgtgttg
      241 gaaggaaggc ccgtgtgtgg gacacgggag cgaggggacc atgagcaatc ttgcacctgt
      301 cgttttcgat ggcgcgccgt cgcccggccg tggtgcgcgc gcgcaccgtg gtgttgcagc
      361 cttgcgccag gccggcgcaa gcggcagcta tgacgcgctg gcgccgatgc tgcggcgcct
      421 gctgcgcaat gcctgcaccg tgctgggcgc gagccagggc ttcttcgtgg tgtcgcgcag
      481 cggcatggcc tacgacatcg cctgcgcgca caacatgcgc ccgtcggaag tgatggacgt
      541 ggcgctgcac gacgccgctg cgccgctgtc ggccacgctg tcggaacggc tggtggccgc
      601 cggcgacctg tgcggtgaaa ccatgccggt ggatgacggc ctgttcgacg cgcattcgcc
      661 cgccgtgctc tgcgtgccgc tggaactggg cgcgcaggaa aatggcgcca tgtgcctgct
      721 gcgcctgcag gcgcgccagc gcatctccga tctcgatctc gaaatcatcc aggcggtggc
      781 cgagcaggcc gcgctggcgc tgaccgccgc ccggcagacg cgcgcgctga cccgcctgca
      841 ggccagcctc aattcgctgc ccgccgcctg cgcctgactg ccgcagccgc gggagtagac
      901 tgcgcgcatg cgcaaaaacc tgcggttccg caacgaggac ccggaagcat tccgccaggc
      961 ggtgctgcgg caggtgcagg aactgaccgc cgccaacgag cgcctggtcg cctcggtggt
     1021 gaagaacggc aagcgcttcc gctcgctggc acgctccgtc tggcatgtgc aggaagagga
     1081 acgccgccgg ctggcgcgcg aactgcacga cggcctgggg cagacactga ccgcgctggc
     1141 caaccagttg cagcgcatcc atgacgacgc cgccgcgcaa tccaacctgg gcctcggcca
     1201 ccggctgagc gacgcgctcg acatcacgcg cggcgcgctg cacgacacgc gcgagctgtc
     1261 gcgcctgctg cgccccaccc tgctcgacga ccttggcctg gaagccgcgc tcaagtggct
     1321 gggtcgcacg ctgggcgagc gcgctgggct ggccatcgaa gtgcattcca ccctcggcga
     1381 tgcgcgcatg gcgccggacc tggagacgct ggtctatcgc atcacccagg aagcgctgac
     1441 caacgtgatc aagcacagcg gcgccaagca ggcgcagatc gtgctcacgc gcgcaccgca
     1501 ggaactgaaa ttgctggtgc atgacgacgg ccgcggcttc gagccggccg cgcaggccac
     1561 gccggagaaa ggcgcgctgg ccggcggact gcgcggcatg cgcgaccgcg ccgaactgtt
     1621 cggcggccgc gtgcgcctgc gctcggaacc gggtgaaggc accagcgtgg aactggcgct
     1681 gccgatcgac ggcgatgatt ttctacatgg agcaggcgca tgagcgcagc caatccgatc
     1741 aagctgatcg tggtcgacga ccacaacatc gtgcgcgagg gcctggtgtc gatgctggag
     1801 gagaccggcg agtgccaggt gctggcgcag gcggccgatg gcgccgaggc gatggaactg
     1861 gcggcgaaac atcgccccga cgtggtggtc accgacctgt cgatgccgcg gctgtcaggg
     1921 ctggaactgg tgcggcgcat ccgcgaaaag atgccgcagg tgaagacgct ggtgctgacg
     1981 gtgcacgagg aagacgagta catcgtgccg atcatccgcg ccggcgcctc gggctttctc
     2041 aacaaggaca cctcggtcgg cgagctgatg acggcgatca agaccatcca cgccggccag
     2101 ggttatttcg gtgcgcgcgc caaggaggcg ctggcggcgc agcaggcgcg cccgcgcgag
     2161 gccgggctca atccttatga cacgctgacc gcgcgcgagc gcgagacttt ccagctggtg
     2221 gtggaaggca agaccaccaa ggagatcgcg cagattctct ccatcggcgt caagaccgcg
     2281 gagaaccacc gcgcgcgcat gatggagaag ctggccctgc gcaacaccgc cgaggtggtg
     2341 cgctttgccg cgcgcaaggg tttgctgccg tagtagatta ggcgcatgca cggcgccgcg
     2401 gcggcgcccg gagtaatgca atgcgacacc tcaccctgct gatcctgctg tcggcgttca
     2461 ccaccggcgc acacgccggc gtgtacaaat gcaccgagaa cggccagacc tttttttccg
     2521 acaagccctg cgcctccaag gaagtgcgcg agaccggccg catggacgac aagccggtgc
     2581 tgtccatcgg tgcgcagaat ggcgcggcca atgccagcgg cggcacgcac acgtccacct
     2641 cgaacgttcc gctgcccggc ccgatcgatt tcggccagga gcccgtggag aggctgaaca
     2701 aggcgatggc catcctcaac agcgtgcaga gcgacggcca gaattgcgat gccgcgctga
     2761 aatcggcgcc gaacgacgcc cgcacaacgg tgctgtgcaa gaacttcatc gcgcagctgc
     2821 gcgatggtgc gcaatggccg caggcggtgg accagatcaa ggaactggtg aaggacagtc
     2881 gcctggtcga ggacaactac gatgcgttca agcagtcgcg cgtgatcaac gagaacatca
     2941 acgcgctgtg gaagaccgcc aagctcgggc tgcacgagag ctgagttcca gcccggcggt
     3001 acgcgggtac gcaaaaaagc ctccaccgca ccgcccctcg gcgtgttatt aaatattgac
     3061 tatatttaaa ataaaacaat aattaataac atcgaaactg ccggtgcggt ataggtgccg
     3121 cggacgtttc cgctccgttt tctgccgcaa cctgcaagca gcgcgagcgc aacagttctc
     3181 ttcgatgcga cgctcgattt ataattgcgc gattcactgg gaaccgccgg cacacgcacc
     3241 cgccgttcgc ggtttttccg aagaagtttc caaactcctg acaggggcat cgttcaatga
     3301 gtaatgaagg cgtggatgcc aaacggcgcc gcttcctcac gcttgcgacc acggtcgtcg
     3361 gcggtgcggg cgtggtgggc gcggcaatac cgtttctggc gtcctggagc ccgtccgagc
     3421 gcgccaagac gctcggcgcg ccggtagaag ccgacatcgg ccagatcgaa ctcgggcaga
     3481 tgattacctt cgtgtggcgc ggccagccgg tgtgggtggt ccaccgcacc aaggaaatgc
     3541 tcgatggcct gtcgaaagtc gacggcgacc tggtcgatcc cgagtccaag gccaagcagc
     3601 agcccgacta catcaagggc gaggcacggg cgatcaagcc cgagtacatg gtgctcaagg
     3661 gcgtgtgcac gcacttgggc tgctcgccga aattccgccc ggatcacccg gcaccggaga
     3721 tcgcacccaa ctggcagggt ggtttcttct gcccgtgcca tggctccaag ttcgacctgt
     3781 ccggccgcgt gtacaagggc gtgccggcgc cgctcaacct cgtggtgccg ccgcattact
     3841 acaaggacga ccacacgctc gtgatcggcg ccgactccaa caagggagcc gcgtaatggc
     3901 cattgttccc aatccgcaca agaccaccgg ctttctcggc tggatcgacg accgcttccc
     3961 gctgaccaag ctgtggaagg accacctggc cgagtacccc gcgccgaaga acttcaacgt
     4021 ctggtacgtg ttcggctcgc tggcgctgct ggtgctggtc aaccagctgc tcaccggcat
     4081 ctggctggcc atgcattaca agcccgatgc caaccaggcc tgggacagcg tcgagtacat
     4141 catgcgcgac gtgccctggg gcaacgtgct gcgctacctg cattccaccg gcgccagcgc
     4201 attcttcgtg gtggtgtacc tgcacatgta ccgcggcctg ctctacggca gccaccgcaa
     4261 gccgcgcgag ctgatctggc tgttcggctg cctgatcttc ctggcgctga tggccgaggg
     4321 cttctgcggc tacgtgctgc cctggggcaa catgagctac tggggcgcgc aggtgatcat
     4381 caacctgttc ggcaccattc ccttcgtcgg gccggacctg gtggtgtgga tccagggcga
     4441 ctacatcccg gccgatgcca cgctcaaccg cctgttctcg ctgcacgtgg tggcgctgcc
     4501 gttcgttatc gtcggcctgg tggtggcgca catcatcgcg ctgcatgaag tgggctcgaa
     4561 caaccccgac ggcgtcgaga tcaagaagaa gaaggacgcc aacggctggc cgctcgacgc
     4621 ggtggcgttc cacccgtact acacggtaaa ggacatcctg ggcgtggtga tcttcctgat
     4681 catcttcctg ggcatcgtgt tcttctctcc ggatggcggc ggctacctgc tggagaagcc
     4741 caacttcacc ccggccaatc cgctgtcgac acccgagcac atcacgccgg catggtactt
     4801 cggcgccttc tacgccatgc tgcgcgccac gccgtcgttc gccggcacgc agatctgggg
     4861 cgtgatcgtg atgttcgccg cggtggtggt gctgttcttc ctgccgtggc tggaccgcag
     4921 cccggtcaag tcgatccgct acaagggcac gctgtcgaag atcgccatcg ccatcttcac
     4981 cgtcaccttc atcatgctca actacctggg catgcagaag cccagcacca cggccacgct
     5041 gctggcacag atcggcacca tcctgtattt cgccttcttc gccgccctgc cgttcattcc
     5101 gcagttcgag caaaccaagc cggttccgga gaggaccacg ccatgaaggg aatttttctc
     5161 ggactcgccg cgctgacgct gtcactgggc gcccacgccg aaggcgaagc gctgccgctg
     5221 cagttcaagc tgcagccgga caacatggcg gccgcgcagc gcggtgcgcg cgacttcatg
     5281 gcctattgct cgggctgcca ctcgatgcgc aacctgcgct acagccgcct gggccgcgat
     5341 ctcggcattc ccgacgacaa gctcaaggcc aacctgatgt tcaccagcga caagcccggt
     5401 gaccagatca agtcggccat gccggcggac gacgccaaga agtggttcgg gcagacgccg
     5461 cccgacctaa ccgtggaagc gcgcatgcgc ggcccggagt ggctctacaa ctacctgatg
     5521 accttctacg tcgatccgac gcggcccacc ggcatgaaca acctggtgct gcccaacgct
     5581 tcgatgccgc atgtgctgtg ggaactgcag ggcctgcagg cgaagaaggc cgagccacgt
     5641 ctgggcgaag gcaatgcgga agccgcggca gagcatgaag gcggcaccgg cctcgaactg
     5701 gtgcagcccg gctcgcagac gccggagcag tacgagaaga ccgtggccga catcgtcacc
     5761 ttcatggcct acgcggccga gccgggccgc gcgcaacgca tcgccaccgg cgcatgggtg
     5821 atggcgtacc tgatcttcct gctgctgccg ctgacctatt tcctgaagaa ggaatactgg
     5881 aaggacgtgc actgatccgc gcgcaattgg ttttgcaggc gccgttcatg gtaataacac
     5941 caccatgaac ggcgttttca tttgcggcgg cacctgatgg tcacgctcac gcgcagcaag
     6001 tacgtgatgg cggaaaagcc ggccgcgcgc gccggcgtca cgctgttcgc ctcgaaacag
     6061 agcctgtcgt gcctgtgggt acgcctggtc ctgctggaaa aggacgtcga gggcgcgcgc
     6121 gtcgaggaag tgttgcccgg ccgccccaat cacgatctcg ccatcctcaa cccggcactg
     6181 acgctgccca cgctggtgga ccgcgaggtg gtgctgcatc ccgcgctggt catcgtcgaa
     6241 tatctcgacg aacgctatcc gcatcccaag ctgctgccgt cagacccggt ggcgcgcgcg
     6301 cgcatccgca tgaccatcca gcacatcgaa agccagctgt atgctctggt cggcgacatc
     6361 cgcgcgcgcg gcgccggcgc caaggccgcg cgcaagcagc tggccgagca gttgaccggt
     6421 gctgccggca tgttcccgca gcgcggctgg ttcctcggcc aggaatacaa cctcgccgac
     6481 tgcgcctggg ccatcctgct gcacacgctg acccagctcg acctgcagtt gcccgggaat
     6541 ctgcaagcca tggtgcgcta tgccgagcgc ctgttcgcgc gtcccgccgt gcagaagctg
     6601 ttgcgatgag cacgtcgcgt cgcccctaca tcatccgcgc catctacgaa tgggcagtgg
     6661 acaacggcta cacgccgcac ctgctggtgg cggccgacta cccgggtgtc agcgtaccgc
     6721 gcgagcacgt ccgcgaaggc cgcatcacgc tcaacgtcag cccgatggcc gtgcagtcgc
     6781 tggatctgaa agccgacccg atctggtttt ccgcacgctt ctccggccat gcctacgacg
     6841 tgctggtccc tccaggcgcg gtgctcgccg tgttcgcacg cgagaacggc gaaggcttca
     6901 tgttcggcga gatcgaaccg cccgatccgg cgaaagcggc ggcgggcgat acgcctcaga
     6961 cgccgccgac caagccggcg ggcggcaagc caaaactgcg gatcgtgaaa taagtcgggc
     7021 tcagcgcgac gtggcccact gggcataatg gtgtcgcgca cagaactcgt cgagttcgcg
     7081 ggccaggtcg cgatcgaggg aagcaatctg cgcggccgac agttgcggct tcccggccgc
     7141 gaacttttct tttgatgaat tgatggtccg aggaacatcg gcgagatcgg cccacgcagc
     7201 ggacccgtcg gctgacggtg gcggcggcag ggctgctgca atcgcgcgca ggtcttccgt
     7261 cgcatgctca atccggatca ctgcgagggg cttgcgcatg tcgacaatgc gcatccagcc
     7321 aagatacgac gccaccgccc ggtccatcgc ggtccggtag cttgcgatgt ccatgccgct
     7381 ggtgcgcagg atgtggaagc gccggaacga gaatgatgcg tcgtcggtat tttccagcat
     7441 gatcgacggg atggcctgcg cgggatcgcg cacataggcg agcaggcaat cgacccgcgc
     7501 cgcagctgcg gcaggcggag gcgtaccgta cggcgggcgg gcgtcatcaa ccgtccatgg
     7561 ccagctgcag atcccatcat cgccgcaatg ctcgtggccg atgtgcaagc caagcctgcg
     7621 cagcacttcg gtcaggtagg ctgagccgca acgtggatgg ccaaccgcaa cgacgcgaaa
     7681 gtggtggctg gccgtcggca gcgggcaatc gagggattcc agttccgcgg cgatgttgcg
     7741 gcgacgggtg tcgtccgatg cgcagcgcgg tgccggcgac tcgtcacagc cgggaagcat
     7801 ggccgcaaat ggctgccctg cgagcaacga ggcggccgga tcgaaccgcg tgtattgatg
     7861 tgcgctttcg gctcgcgtgg caaagcccgc ccagtggtcc atgatgcttc ccacgtccat
     7921 tgcaatgcca ccgagatcgc ggatacgggc acacagcagc ttgcccagca tgccggcacc
     7981 aaccaggaac acttcaccgc gatcaaactc gatgtccgcg aagatgcgct ggaacaccgt
     8041 cggataaaac ggttcgccct tgccctggaa ccccatgagt ccggcatcga gccgttccgc
     8101 cggaatgttc cgacgccgcc agcaaatctc ccgcatcggc caattcgcgc ccgcgccaga
     8161 gccgatacgc gtaggaatcg tccacgccgg attcagcctt gccgccgtgc atctgcatcc
     8221 gtcctttctg gattcagatt ccgggaaggc atttgccggg attgagaatg cccctggggt
     8281 ccatcgcgcg cttgagttgc cgcatcgtct ccagcgtgtc agcagcgatt tcccagccga
     8341 cgtaatcgcg tttctcgatg cccacgccat gctcgccgga gatggtgcct tccagcgaca
     8401 gcaccaggcg gaacacgccg tgcaggcagg gctcgatcac tttcatctgc gccgggtctt
     8461 caggatcggc caggatgttg acgtgcaggt tgccgttgcc ggcgtggccg aagttgacga
     8521 tgcgcaggcc gtgttgccgg gacagctggg tgaggcccgc gatcagttcg ggcaaacggg
     8581 tcaccggcac cgccacgtcc tcgttcacct tcttcggcgc gatcttgcgc aggctcggcg
     8641 gcagcgcctt gcggcaggcc cacaaggcgg cggtttcggc gtcgctgccg gccatcttca
     8701 gctccaccag tccatcaccg ttggccgcct cacctactgc tcgcaccgca tgatcgatgg
     8761 cttcgcgcga gccatcgacc tcgatcacca gcagtgcgcc ggtgccagcg ggaacaccgg
     8821 tgtcctggaa ctgttgcgcg agcatgaccg catcgccatc catgaactcc agcgcgctcg
     8881 gcgtctccgg ctgctgcatc acccgcgcca cggccgccgc cgcgctgttc acgtcgacgt
     8941 agcaggcgcg cagcgtgcgc gtaaccggcg gcttcggcag cagcttgagc gtggcctcgg
     9001 tgatgatggc cagcgtacct tcgctgccga cgatcaggcg cgtgaggtcg taacccacca
     9061 ccccctttgt tgtgtagcag ccggtcttga gcgatgcgcc ggtgccgccg atggcgcgca
     9121 gccccagcac gttgtcgcgc gccgtgccgt acttcaccgt gcgcggtccg cccgcattgc
     9181 aggccaggtt gccgccgatg gtggaatatc cggccgacgt agggtccggc ccccagaaca
     9241 agccgtgctt ttccgcgacc gcctgcgcgt cggtgttgat cacacccgcc tcgcactcca
     9301 tcatgcggtc gcccggcgac acgcgcagca cacggttcat gcgttcgagc gacagcacca
     9361 cgccaccggc gaccggaacc gttgcgccgg tggtgttggt ggcgcggccg cgcgcgatca
     9421 gcggaatgga aaactcgttg cacgctgcaa ccaccgcggc cgcttcgtca tgggtcagtg
     9481 cgaacgccac cgcctgcggc agtagcatgc gcttgctgtt gtcatagccg taggccaggc
     9541 aatccgccgg atcggtgtac aggcgctcgc gcggcaggat ctctgcgcag cgctggagaa
     9601 atgcagacgg cagcggctca ggcatcgaac gctcccggaa gccactcgat gcggttggcg
     9661 gcatcgagtg caatcacatc gaagcgcacg ggacagtgcg cgaactcgct gtgtgaggcc
     9721 aggaagctct gcgcggcatg gacgatgcgt gcctgcttgc gcccggtgac cgattccgca
     9781 gcgctgccga aattcgggtt gccgcgcttg cgcacctcca cgatcaccag catatcgcgc
     9841 tcgcgcatga ccaggtccag ttcgccgccg cggcagcggt aattgcgctc gaccagttgc
     9901 atcttctgcg cctgcaggta ggcgagcgcc aggttctctt cctgcgcgcc cttcacagcc
     9961 cgctgccaga tgccgccagc ggtgtcagct tgccgctgtc gatctgcgca cattgcagac
    10021 gccgcgcgat cacgccattg ctgcccaacg ccagttcgcc ggttgctgcg ccgaagctgg
    10081 caccatgctg cagctggccg gaagcgatca cgccggcgag cgcgtaagcg tcatagccca
    10141 tggcgaacag gcgcggctgg aaacgggcgc caggaacttc ggtggtttcc gcatgcaggt
    10201 tttccagcgg cgcggcgccg ccgagcatcc acggcatgtc acagaacgcc agccccgcca
    10261 ggtccggctc ggccgtgccg ctgtagatca gcgaggtggc ataagcggca aggtcacccg
    10321 cgccgttgta gcgcagctgc ggccagatca agcggccttc cgagggccgc gcggccagga
    10381 acaccacgtc gatgtcgtcg cgccggtgcg gctcgaactc ggtcttcttg ccgagcagcg
    10441 aggtgatggc gcgatgccgc tcgttgctgg catcgaccga aagcagctcc tggatcggca
    10501 cggtgaagtc gcgcagcgat ggatcgtagc gcgcagcttt cagcacatgg ccgccgagct
    10561 cggtgaaccg ctggctgaat gcggccagcg cgcgttcgcc ccagtccgcc ttcggcacca
    10621 gcgccactgc gctgcgcaag tcgcggccga tggcgttttc cgccgcggcg cgcgcttcat
    10681 cttccggcgc caggccgaac tggtagaaat tcgaaggcga cgctgccggc tccaggtagt
    10741 taagggccag caccggcgtc gacagcacgc cgacgttggc gatggccacc accgattcct
    10801 tgcgcagcgg accgaccacc aggctggcgc cgtcgtgcaa tgcctgctgg taggccgcca
    10861 cgctggcagc cggcgcggag ccggagtcgt aaatgcgcac cggcgcggcg ctggcgcccg
    10921 cggcgaagcg ggcggagaga aaaccatcgc gcaccgcctc gccgctcgcc gcggtggggc
    10981 ctgtcagcgg cagcagcagc gcgaccagtc ccttgggccc ggcctgcggc agcggcacac
    11041 tggccggcgc ggcggccgct gcagctggcg ccaccaccgg gtgctcggca atgctggcga
    11101 tgcgctccgc acccggatga cccgggtagc gcgtgcgcca gctttccaca tcgccgtgct
    11161 c
//
LOCUS       DASZZC010000348         6388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1124040,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000348 DASZZC010000000
VERSION     DASZZC010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124040"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VHE37_07370"
     CDS             complement(<1..325)
                     /locus_tag="VHE37_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35384.1"
                     /translation="MTDPTKTSAWSALQQHARESSATISELFARDANRAADFSAEGAG
                     IFLDYSKQRLDAQARGLLLELAQQQGMADAIRRMFAGEAINQTEQRAALHVALRGSGP
                     QRAQHE"
     gene            complement(421..804)
                     /gene="panD"
                     /locus_tag="VHE37_07375"
     CDS             complement(421..804)
                     /gene="panD"
                     /locus_tag="VHE37_07375"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HVT35385.1"
                     /translation="MQLNMLKCKLHQARVTHAELDYDGSCAIDGKLLDLAGILEFEQI
                     QIYNINNGERFTTYAIRAEEGSGIISVNGAAAHKARVGDRVIIAIYAAMDEAAAKAYK
                     PRLVYCDAQNRVARTANTIPVQMAA"
     gene            complement(965..1813)
                     /gene="panC"
                     /locus_tag="VHE37_07380"
     CDS             complement(965..1813)
                     /gene="panC"
                     /locus_tag="VHE37_07380"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HVT35386.1"
                     /translation="MKTVHAIAELRAQLHEWRRAGQRIALVPTMGNLHRGHLELVRRG
                     REVADRVVASVFVNPLQFGPNEDFNRYPRTLPADQAGLERAQADLLFAPSAAEIYPRG
                     MERLTRVNVPAVSDILCGHFRPGHFEGVATVVSILFNLVQPDAALFGEKDYQQLYIIR
                     RMVADLHMPIDVVGVATEREPDGLALSSRNQYLDAQERKLAPELYATLTEVADKLKAG
                     RRDFSALSYAGIKRLEATGFRPQYLEVLNADLVAPAKENNSFVVLVAAYLGRTRLIDN
                     VSVTAL"
     gene            complement(1825..2721)
                     /gene="panB"
                     /locus_tag="VHE37_07385"
     CDS             complement(1825..2721)
                     /gene="panB"
                     /locus_tag="VHE37_07385"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVT35387.1"
                     /translation="MEPAKPVNVAELRRMREAGERIACLTCYDASFALIEDRAGVDVV
                     LVGDSLGMVLHGGTTTTPVTVDDIVYHSRCVAPFLKRAFLMADLPFLSYATLDRALDA
                     SQRMMQEGGAKMVKLEGGREQASIVEYLAVRGVPVCAHLGLQPQFIHKMGAFKVQGRD
                     DATAEAMLHDAKVLADAGADVLLLECIPAALGKKIADASPVPVIGIGAGHDVDGQILV
                     LHDILNISPTVTLGRKPRFVKNYMEGGADIEGAIRAYVNEVKSGAYPAPEHCFADVAA
                     LPNQPGARVAPALPAAKVKQRK"
     gene            complement(2731..3276)
                     /gene="folK"
                     /locus_tag="VHE37_07390"
     CDS             complement(2731..3276)
                     /gene="folK"
                     /locus_tag="VHE37_07390"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HVT35388.1"
                     /translation="MILKMGHTLAENHRASLHMPLAYIGLGANLGDPPRQLRAALAGI
                     GASDGVELVAQSPFYRSAPLGPAAQPDYCNAACAVRTSLPPLDLFARLLAIERAAGRV
                     RDGARWGPRTLDLDLLVYEGVVMDTLQLRLPHPEIARRNFVLAPLADLAPDLHIDGQG
                     EVRALLRAAGNDGLARWNDPL"
     gene            complement(3379..4761)
                     /gene="pcnB"
                     /locus_tag="VHE37_07395"
     CDS             complement(3379..4761)
                     /gene="pcnB"
                     /locus_tag="VHE37_07395"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HVT35389.1"
                     /translation="MQGRSLIEPQRIPRAEHNISRQDISPGALKVLYTLRDAGYEAYM
                     VGGGVRDLLAGIKPKDFDIATSAHPEQIKQLFGRSCQLIGRRFIICHVRVGPEVSEVT
                     TFRGPITESHERDETGRILSDNMYGTLEQDAFRRDFTINALYYDIRDYAVVDFAGGMA
                     DLQARSVRLIGDPDLRYREDPVRMLRAVRIASKLGFTIEAATAAPIPLLAPLLAEIPP
                     ARLFDEVIKVLQSAEAVANYEGLAELGLFAQLFPETDALAEHDDVVDDFIALALANTQ
                     ARVRAAQTVSPAFLYAALLWPPVRRRMQHLTDHEAHHVNAAIAQAGEEVLGRAVMRVA
                     IPKRFSLPMREIWQLQPRFEQRQGGRARRLLAHPRFRAAYDFLLLRARCGEVPQELAD
                     WWTQAQKDTAHLPPATENEADEGADALPAGEGAASGNNGAAPGNGNGPRKRRRRRGGR
                     GRRGGPPAAG"
     gene            4949..>6388
                     /locus_tag="VHE37_07400"
     CDS             4949..>6388
                     /locus_tag="VHE37_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HVT35390.1"
                     /translation="MIKAHAWMLAALLAPGAAGANGAYTQLHDFSGADGKYGQAELLL
                     GSDGAFYGTTTAGGRNDDGVLFRVTTDGAYSVLHHFGPGASRPQGGVIQGSDGAFYGT
                     TYYGGTGDNGSIYRVAPDRSYQRLHAFNGSGGAHPLGALAMDTGGTLYGTTFAGGRHD
                     HGVVFSRSADGTYRRLHDFDDADGANPTSGVVIGPDGALYGVTNYGGAAGLGAAYRIT
                     TDGQYSKLHDFDNAEDNGSRPSPALTLGPDGALYGTTTAGGLHQAGIVFRLATGGAFT
                     KLHDFDLDDYNGYFPYGVLTVTPEGTLYGTTFGGGDIADGAVFRLEPDGTYTKLHDFY
                     GDRGHWPYAGVTLDADGVLYGVASEGGAQGYGTVFRLDARHMPTATITATPASIGPGE
                     TSTINWTSDGATSCFATSGNWFYGEQPAAGSVRRGVSGNINNPGGRFPYALTCSGPGG
                     SVKTSATVTVMQPPPTLEMSVSASHAAVGT"
BASE COUNT          994 a         2165 c         2176 g         1053 t
ORIGIN      
        1 cctcgtgctg cgcgcgctgc gggccgctgc cgcgcagcgc cacgtgcagc gcggcgcgct
       61 gctcggtctg gttgatcgcc tcgccggcga acatgcggcg gatggcgtcc gccatgccct
      121 gctgctgcgc cagttcgagc agcagtccgc gcgcctgcgc gtcgaggcgc tgcttggagt
      181 aatcgagaaa aatgccggcg ccctcggcgg aaaaatcggc cgcgcgattg gcatcgcgcg
      241 cgaacagctc gctgatggtg gcggaggact cgcgcgcgtg ctgctgcagc gcgctccagg
      301 cggaggtctt ggtcgggtcg gtcatggcgg caagagtgac tgacaacttt ttccgcggca
      361 aatccgccgc gccaacaaaa acgcccgcgc gaggcgggcg tcgatgcgaa gcttaaaaac
      421 tcaggcggcc atctgcaccg ggatggtgtt ggccgtgcgc gccacgcggt tctgcgcgtc
      481 gcagtagacc aggcgcggct tgtacgcctt ggccgcggct tcgtccatcg ccgcgtagat
      541 ggcgatgatc acgcggtcgc ccacgcgcgc cttgtgtgcg gcggcgccgt tgaccgaaat
      601 gatgccgctg ccttcctcgg cgcggatggc gtaggtggtg aaacgctcgc cgttgttgat
      661 gttgtagatc tggatctgct cgaactcgag gatgccggcc agatcgagca gcttgccgtc
      721 gatggcgcag gagccgtcgt agtcgagttc ggcgtgggtg acgcgcgctt ggtgcagctt
      781 gcacttcaac atgttgagtt gcatggcgta tgtctcgggg ttttgcagcg cgagcggccc
      841 aaaaagacat gcgctcgtgc tgcgggggtc ttggcagcgg gcggcccaaa aagacatacg
      901 cccgggatcg cgtgttttgg acactgcgat ccggttccaa gtttattcga ttcctgcagt
      961 ttttttaaag cgccgtgacg gagacattat cgatcagccg cgtgcggccc aggtatgccg
     1021 ccaccagcac cacgaagctg ttgttttcct tcgccggggc gaccagatcg gcgttcagca
     1081 cttccagata ctgaggcctg aatccggtgg cttccaggcg cttgatgccg gcgtacgaca
     1141 gcgcggaaaa atcgcggcgg ccggccttga gtttgtccgc cacctcggtc agcgtggcgt
     1201 acagctcggg ggcgagcttg cgctcctgcg catcaaggta ctggttgcgc gaggacagcg
     1261 ccagcccgtc gggctcgcgc tcggtggcca cgcccaccac gtcgatcggc atgtgcaggt
     1321 cggccaccat gcggcggatg atgtacagct gctggtaatc cttctcgccg aacagcgccg
     1381 cgtcgggctg caccaggttg aacaggatgc tgaccaccgt ggccacgccc tcgaaatgtc
     1441 cgggccggaa atgcccgcac agaatgtccg acaccgccgg cacgttgacg cgcgtcagcc
     1501 gttccatgcc gcgcggatag atctccgccg cgctgggcgc gaacagcaga tcggcctgcg
     1561 cccgctcgag cccggcctgg tcggccggca aggtgcgcgg atagcggttg aaatcttcgt
     1621 tcggcccgaa ctgcagcggg ttgacgaaca cgctggcgac cacgcggtcg gccacctcgc
     1681 gcccgcgacg caccagttcg agatggccgc gatgcaggtt gcccatggtc ggcactaagg
     1741 cgatgcgctg gccggcgcgg cgccactcgt gcagctgcgc gcgcagttcg gcgatggcgt
     1801 ggaccgtctt catcggagca cgcgtcactt gcgctgtttg accttcgccg ccggcagcgc
     1861 cggcgccacg cgcgcgccgg gctggttggg cagcgccgcg acatcggcga agcaatgctc
     1921 aggtgccgga tatgcgccgg acttcacctc gttcacgtag gcgcggatgg caccctcgat
     1981 gtccgcgccg ccttccatgt agttcttgac gaagcgcggc ttgcgcccca gcgtgaccgt
     2041 gggcgagatg ttgaggatgt cgtgcagcac caggatctgg ccgtcgacat cgtggccggc
     2101 accgatgccg atcaccggca ccggcgaggc gtcggcgatt ttttttccga gcgcggccgg
     2161 gatgcattcc agcagcagca cgtcggcgcc ggcatcggcc agcactttcg cgtcgtgcag
     2221 catggcctcg gcagtggcgt cgtcgcgccc ctgtaccttg aacgcgccca tcttgtggat
     2281 gaactgcggc tgcaggccca ggtgcgcgca caccggcacg ccgcgcaccg cgaggtattc
     2341 gacgatgctg gcctgctcgc gcccgccttc cagcttgacc atcttggcgc cgccttcctg
     2401 catcatgcgc tggctcgcgt cgagcgcgcg gtcgagcgtg gcgtaggaca ggaacggcag
     2461 gtcggccatc aggaacgcgc gcttcaggaa cggcgccacg cacctgctgt gatagacgat
     2521 gtcgtccacc gtcactggcg tggtggtggt gccgccgtgc agcaccatgc ccagcgaatc
     2581 gccgaccagc accacgtcga cgccggcgcg atcctcgatc agcgcgaagc tggcgtcgta
     2641 gcaggtcagg caggcgatgc gctcgccggc ctcgcgcatg cggcgcagtt cggcgacgtt
     2701 gacgggcttg gcgggttcca tggcgcgcga ctatagcgga tcgttccagc gtgcgagacc
     2761 gtcattgccg gcggcccgca atagcgcgcg cacctcgccc tgcccatcga tgtgcaggtc
     2821 tggcgcaagg tccgccagcg gcgccagtac gaagttgcgc cgcgcgattt ccgggtgcgg
     2881 caggcgcagc tgcagcgtgt ccatcaccac gccctcatac accagcaggt cgagatcgag
     2941 cgtgcgcgga ccccagcgcg cgccgtcgcg cacgcggccg gccgcgcgct cgatggcgag
     3001 caggcgcgcg aacaggtcga gcggcggcag cgacgtgcgc accgcacacg cggcgttgca
     3061 gtagtcgggc tgtgctgccg gcccgagcgg cgcgctgcgg tagaacggcg actgcgcgac
     3121 cagttcgacg ccgtcgctgg cgccgatgcc ggccagcgcc gcgcgcagct ggcgcggcgg
     3181 atcgccgagg ttggcaccca gaccgatgta ggcgagcggc atgtgcagtg atgcgcggtg
     3241 attttcagcc agcgtatggc ccattttcag gatcaccgca tcggcactta ccatgttatt
     3301 aaatattgtc ttttttaact ttcagacaat aataaataac atgcctgcag gcagtgcggt
     3361 gggcctgatt ttgcgccttc agcccgccgc cggcggcccg ccgcggcgtc cgcgaccgcc
     3421 gcgccggcgg cgacgcttgc gcggtccatt gccattgccc ggcgccgcgc cgttgttgcc
     3481 ggatgctgcg ccctcgcccg ctggcagcgc atcggcaccc tcgtctgctt cgttttccgt
     3541 agcaggcggc aggtgcgcgg tgtccttctg cgcctgcgtc caccagtccg ccagctcctg
     3601 cggcacttcg ccgcagcgcg cgcgcagcag caggaagtcg taggcggcgc ggaagcgcgg
     3661 atgcgccagc aggcggcgcg cgcgtccgcc ctggcgctgc tcgaagcgcg gctgcagctg
     3721 ccagatctcg cgcatcggca gcgagaagcg cttggggatg gccacgcgca tcaccgcgcg
     3781 gcccagcact tcctcgcccg cctgcgcgat ggcggcgttg acgtggtgtg cctcgtggtc
     3841 ggtcaggtgc tgcatgcgcc ggcgcaccgg cggccacagc agcgcggcat agaggaatgc
     3901 cgggctcacc gtctgcgccg cgcgcacgcg ggcctgcgtg ttggccagcg ccagtgcgat
     3961 gaagtcgtcg accacgtcgt catgctcggc cagcgcgtcg gtctccggga acagctgcgc
     4021 gaacaggccg agctcggcca ggccttcgta gttggccaca gcctcggccg actgcagcac
     4081 cttgatcact tcatcgaaca ggcgcgccgg cggaatttcc gccagcagcg gagccagcag
     4141 cgggatcggt gcggcggtgg cggcttcgat ggtgaaacca agcttggagg cgatgcgcac
     4201 cgcgcgcagc atgcgcaccg ggtcttcgcg gtagcgcagg tcagggtcgc cgatcagccg
     4261 caccgagcgc gcctgcaggt cagccatgcc gccggcgaaa tcgaccaccg cgtaatcgcg
     4321 gatgtcgtaa tacagcgcat tgatggtgaa gtcgcggcgg aaggcgtcct gctccagcgt
     4381 gccgtacatg ttgtccgaca ggatgcgccc ggtttcgtcg cgctcgtggc tctcggtgat
     4441 cgggccgcgg aaggtggtga cctcgctcac ctcgggcccc acgcgcacgt ggcagatgat
     4501 gaagcgccgg ccgatcagct ggcagctgcg cccgaacagc tgcttgatct gctcgggatg
     4561 cgcgctggtg gcgatgtcga aatccttcgg cttgatgccc gccagcaggt cgcgcacgcc
     4621 gccgccgacc atgtacgcct cgtagccggc gtcgcgcagc gtgtacagca ccttgagggc
     4681 gccgggagaa atgtcctggc gcgagatgtt gtgctcggca cgggggatgc gctggggttc
     4741 gatgagagat cgtccttgga tttttttgaa tcggccagca gcggccggtt gccgtcgctg
     4801 cgccgcgcag tctgcaagca tccgcgcgag atggaaaatt ttaaggcaaa gggattccgc
     4861 catgccagtt ccgccgcgcg tgccggggtg gcagacgcac acggccctgc gctatggtct
     4921 gccggtcaaa gttccgggga gcggcgacat gatcaaagcc catgcatgga tgctggcggc
     4981 gctgcttgcg ccaggtgctg ccggcgccaa cggcgcctac acccagctgc acgacttcag
     5041 tggcgcggac ggcaagtacg gccaggcgga actgctgctg ggcagcgacg gcgcgtttta
     5101 cggcaccacc accgcgggcg ggcgcaacga tgacggcgtg ctgttccgcg tcacaactga
     5161 tggtgcgtat tcggtcctgc accacttcgg gcccggcgca tcgcgcccgc agggcggcgt
     5221 gatacagggc agcgacggcg cgttttacgg caccacctat tacggcggca ccggcgacaa
     5281 cggcagcatc taccgcgtgg cgcccgatcg cagctaccag cggctccacg cattcaacgg
     5341 cagcggcggc gcgcacccgc tcggcgcact ggcgatggac accggcggca cgctttacgg
     5401 caccaccttc gccggcggca ggcacgacca cggcgtggtc ttcagccgca gtgcggacgg
     5461 cacctaccgg cgcctgcacg actttgatga cgccgatggc gccaatccca cctccggcgt
     5521 cgtcatcgga cccgacgggg cactgtacgg cgtgaccaat tacggcggcg ccgccgggct
     5581 cggcgcggcg taccgcatca ccaccgacgg ccagtacagc aagctgcacg acttcgacaa
     5641 cgccgaagac aacggcagcc gtccctcacc cgcgctcacg ctggggccgg acggcgcgct
     5701 gtacggcacg accaccgcgg gcggcctgca ccaagccggc atcgtgttcc gtctcgccac
     5761 cggcggcgca ttcaccaagc tgcacgactt cgacctggac gactacaacg gttacttccc
     5821 ctacggcgtg ctcacggtga cgccggaggg cacgctctac ggcaccacct tcggcggcgg
     5881 cgacattgcg gatggcgccg tgttccggct cgagcccgat ggcacctaca ccaagctgca
     5941 cgatttctac ggcgaccgtg gccattggcc atatgccggc gtcaccctgg atgccgatgg
     6001 cgtgctctac ggcgtggcca gcgaaggcgg cgcgcaaggc tacggcaccg tgttccgcct
     6061 cgacgcgcgg cacatgccca ccgccaccat cacggccacg cccgcttcca tcgggcctgg
     6121 cgaaacatcg accatcaact ggacttcgga cggcgccacg tcgtgctttg ccaccagcgg
     6181 caactggttc tacggcgagc agcccgcggc aggcagcgtg cgccgtggcg tcagcggcaa
     6241 catcaacaat cccggtggac gtttccccta cgcgctcacc tgcagcggac cgggcggctc
     6301 ggtgaaaacc tccgccacgg tcaccgtgat gcagccgccg ccgacgctcg agatgtccgt
     6361 cagcgcaagc catgctgccg tcggcacg
//
LOCUS       DASZZC010000349         3966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_858315,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000349 DASZZC010000000
VERSION     DASZZC010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858315"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VHE37_07405"
     CDS             complement(<1..569)
                     /locus_tag="VHE37_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVT35391.1"
                     /translation="MSPTFRRLRHLAPAALALSAFAAQAGDLPPVEEQSAVQLQARLQ
                     AGEISAHDLVQAYLARIEALDQGGPRLNSVIEVNPDALPIADALDAERAQGQVRGPLH
                     GIAVLIKDNIDTADGMLTTAGSQALAGSRPSQDAAIVKRLRAAGAVVLGKTNLSEWAN
                     FRSSRASSGWSGRGGQTRNAYDAARNPCGS"
     gene            complement(654..2798)
                     /locus_tag="VHE37_07410"
     CDS             complement(654..2798)
                     /locus_tag="VHE37_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT35392.1"
                     /translation="MKRILQLGALALGAGAACAAPAQDTPATTDDGATRLPTIVTEEK
                     PWNFNKETRYSHLLPEVDGTAITVTKKNSVTRLDEQPTIIDNNQREVFERVPGLVIAE
                     QQNPGQLNINYRGIGGNGALGTQESELLLSLQDGMPIASDWIGFPTTYYLPVPQSIQE
                     IQFIRGSSGLIYGPEPAALNYVSRKPDADHALAGYSEHVTGSNALYSTFNSVSGTSGD
                     WNYLANAHFRHADGERANGQYQVEGGDAHLGYALSSSQRLAFDFHAYSADDGEAGRMS
                     LTQFQANPYQTTTPYDHDWVRRFTGALTHDYQPDEQLLVETKVWGGFHNQSERNQNGA
                     GTATTLVVQPFHFVGVDSRVRMRWGRGNALTAGAVFYDSSSPMLRYSSSNLSVDPGDR
                     SGTPLVDQQRESKYGAVFAENVFRFGRFHVVPSVRLEREVLDINEPLPPAGRAAINQA
                     FARGVPLFGLGFGNDFGHGNETYVNISQGYRPMRFLDVAPTSASVNPSLNNPAPSKSL
                     TYEAGVHGWPLVGLYYDFSIFQTNFTNRIESQQVGPLPTDKANVNTGDTRHRGVEGQV
                     EYDFMKLFKPESDAHLSGFLNASYLDAEFTEAHAAGVSVGNDPSYAPHYVVREGVLWR
                     KDRAWKFSLSAQTIGSQYWQDSNAAAGGITKIPSYTVADFGADIWLMRNLRLLGGVSN
                     LADRTYYSRVLFGGSIEPAAGRTFYAGLSAEF"
     gene            complement(2795..3010)
                     /locus_tag="VHE37_07415"
     CDS             complement(2795..3010)
                     /locus_tag="VHE37_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35393.1"
                     /translation="MLQMMRFMILPLLVVVVALRRGPRRLRIIGERFAAVNQGFPPSV
                     DLLFTKIDIVLTCAAAAGSRTPKRGKT"
     gene            2988..3602
                     /locus_tag="VHE37_07420"
     CDS             2988..3602
                     /locus_tag="VHE37_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVT35394.1"
                     /translation="MNRIICSIAACAGMAVAGGACAGQPAGNLDIYYLASSRLDLEVS
                     GIGSATFDGDGFGVRGRVNLPPNFFLHGAYQQGNHDKNGVNADLDDLRLGGGAQIPAT
                     PFYGSLELVNLKLSSGGSSSSESGFGIHAGALLQFDALSLNAEVGYLSVGDAGNGAEY
                     LVGGAFNFTPQWAFVADYRVTQLEKDGVTTKPQDARVGVRYNFR"
     gene            complement(3658..>3966)
                     /locus_tag="VHE37_07425"
     CDS             complement(3658..>3966)
                     /locus_tag="VHE37_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HVT35395.1"
                     /translation="LRLNQPIIQAVNYLAYPLQLLLLLPFYRAGETLFGQPHLPIFSV
                     SELLARFQAGPLQFLVDYGMVGIYGIVVWCLLAPIAFVLIRRATLLPLRALAALNARP
                     "
BASE COUNT          620 a         1293 c         1395 g          658 t
ORIGIN      
        1 gagccgcagg ggttacgcgc ggcatcgtag gcattgcgtg tctggccgcc gcgcccgctc
       61 cagcccgacg acgcgcggct ggaacggaaa ttcgcccact cggagaggtt ggtcttgccc
      121 agcaccacgg cgcccgccgc gcgcagccgc ttcacgatgg cggcgtcctg cgatggccgg
      181 ctgccggcca gcgcctgcga accggccgtg gtaagcatgc cgtcggcggt gtcgatgttg
      241 tccttgatca gcacggcgat gccgtgcagc ggtccgcgca cctggccctg cgcgcgctcg
      301 gcatcgagcg catcggcaat cggcaaggca tctggattga cctcgatgac ggaattcagc
      361 cgcggcccgc cctggtccag cgcctcgatg cgcgcgaggt aggcttgcac caggtcgtgc
      421 gccgagattt cgccggcctg caggcgcgcc tgcagctgca cggccgactg ctcttccacc
      481 ggcggcaggt cgccggcctg cgccgcgaat gccgacaggg ccagcgcggc gggcgcgaga
      541 tgtcgcagac ggcggaaggt cgggctcatc ggtaccgcag tgtagctgct gccgccgcgc
      601 cgcctaaatg aaaaggcccc gctggtgcgg ggccctggtt tcaccggcgg gcgtcagaac
      661 tccgcggaaa gaccggcgta gaaggtgcgg cccgccgccg gctcgatgct gccgccgaac
      721 agcacgcgcg agtaataggt gcggtcggcc aggttggaca cgccgccgag caggcgcagg
      781 ttgcgcatca gccagatgtc ggcgccgaag tccgccaccg tgtacgacgg gatcttggtg
      841 atgccgccgg cagcggcgtt gctgtcctgc cagtactgcg agccgatggt ctgcgcgctc
      901 aggctgaact tccaggcgcg gtccttgcgc cacagcacgc cctcgcgcac cacgtagtgc
      961 ggcgcgtagc tggggtcgtt gccgacgctg acgccggccg cgtgcgcctc ggtaaactcg
     1021 gcgtcgaggt agctggcgtt gaggaagccc gacaggtgcg catcggattc cggcttgaac
     1081 agcttcatga agtcgtactc gacctggccc tcgacgccgc ggtgacgcgt gtcgccggtg
     1141 ttgacgttgg ccttgtcggt cggcagcggg cccacctgct gcgactcgat gcggttggtg
     1201 aagttggtct ggaagatgct gaagtcgtag tacagcccca ccagcggcca gccatgcacg
     1261 cccgcttcgt aggtcagcga cttgctcggc gccgggttgt tcagcgacgg gttgacgctg
     1321 gcgctggtcg gcgccacgtc gaggaagcgc atcggccggt agccctgcga aatgttgacg
     1381 taggtttcgt tgccgtggcc gaagtcattg ccgaagccca ggccgaacag cggcacgccg
     1441 cgggcgaagg cctggttgat ggcggcgcgg ccggccggcg gcagcggctc gttgatatcc
     1501 agcacttcgc gctccaggcg caccgacggc accacgtgga agcggccgaa gcggaacacg
     1561 ttctcggcga acaccgcgcc gtacttgctc tcgcgctgct ggtcgaccag cggcgtgccg
     1621 ctgcggtcgc ccggatcgac cgacaggttg ctcgagctgt agcgcagcat cgggctgctg
     1681 gagtcgtaga acacggcgcc ggcggtgagc gcgttgccgc ggccccagcg catgcgtacg
     1741 cggctgtcga cgccgacgaa gtggaacggc tgcaccacca gcgtggtggc cgtgccggcg
     1801 ccgttctggt tgcgctcgga ctggttgtgg aagccgcccc acaccttggt ctccaccagc
     1861 agctgctcgt cgggctggta gtcgtgcgtc agcgcgccgg tgaagcggcg cacccagtca
     1921 tggtcgtacg gcgtggtggt ctggtacgga ttggcctgga actgggtcag cgacatgcgg
     1981 ccggcctcgc cgtcgtcggc cgagtaggcg tggaaatcga acgccagccg ctgggacgac
     2041 gacagcgcgt agcccaggtg ggcgtcaccg ccttcgacct ggtactgacc gttggcgcgc
     2101 tcgccgtcgg catggcggaa atgcgcgttg gccaggtagt tccagtcgcc cgaagtgccg
     2161 gacaccgagt tgaaggtgga gtacagcgcg ttgctgccgg tgacatgctc gctgtagccc
     2221 gccagcgcgt gatcggcatc gggcttgcgg ctcacgtagt tcagcgccgc cggctccggc
     2281 ccgtagatca ggcccgagct gccgcggatg aactggattt cctggatgct ctgcggcacc
     2341 ggcaggtagt aggtggtggg gaagccgatc cagtccgagg cgatcggcat gccgtcctgc
     2401 agcgacagca gcagctcgga ttcctgcgtc cccagcgcgc cgttgccgcc gatgccgcgg
     2461 tagttgatgt tgagctggcc gggattctgc tgctcggcga tgaccaggcc cggcacgcgt
     2521 tcgaatacct cgcgctggtt gttgtcgatg atggtcggct gctcatccag ccgcgtcacc
     2581 gaattcttct tggtcacggt gatggcggtg ccgtcgactt ccggcagcag gtgcgagtag
     2641 cgcgtctcct tgttgaagtt ccacggcttc tcttcggtga cgatggtcgg caggcgcgtg
     2701 gcgccgtcgt ccgtggtggc cggcgtgtcc tgcgccggtg cggcgcaggc ggcgccggcg
     2761 cccagcgcca gtgcgcccag ctgaagaatg cgcttcatgt ttttcccctt tttggcgtgc
     2821 ggctcccggc cgcggcggcg cacgttaaca caatgtctat ttttgtaaac aacaggtcaa
     2881 cgctcggggg aaaaccctga ttgaccgcgg caaaacgctc cccaatgatg cgcaggcggc
     2941 ggggaccgcg ccgcagggca acaacaacaa ccagcaaggg gagaatcatg aaccgcatca
     3001 tctgcagcat cgctgcatgc gccggcatgg ccgtcgccgg tggcgcttgc gcggggcaac
     3061 ccgccggcaa tctcgacatt tattacctgg ccagttccag gctggacctg gaggtcagcg
     3121 gcatcggctc cgccacgttc gatggcgacg gctttggcgt gcgcggccgg gtgaacctgc
     3181 cgccaaactt cttcctgcac ggcgcctacc agcagggcaa ccatgacaag aacggcgtca
     3241 acgcggacct ggacgacctg cgcctgggcg gcggcgcgca gatcccggcc acaccgttct
     3301 acggcagcct ggagctggtg aacctgaagc tgagcagcgg cggcagctcg agcagcgaat
     3361 cgggcttcgg catccatgcc ggcgcgctgc tgcagttcga tgcgctgagc ctcaacgccg
     3421 aggtcggtta cctgtccgtc ggcgatgccg gcaacggcgc cgaatacctg gtcggcggcg
     3481 ccttcaactt caccccgcaa tgggcctttg tcgccgacta ccgcgtcacc cagctggaga
     3541 aggacggcgt caccaccaag cctcaggacg cgcgcgtcgg cgtacgctac aacttccgct
     3601 gatcggattg cacgctggcg tcacgcaggg ccccgccgcg gcggggcctt ttctttttca
     3661 agggcgcgcg ttgagcgcgg ccagcgcacg cagcggcagc agcgtggcgc ggcggatcag
     3721 cacgaaggcg atgggcgcca gcaggcacca gacgacgatg ccgtaaatgc cgaccatgcc
     3781 gtagtcgacg aggaactgca gcggccccgc ctggaagcgc gccagcagtt cgctcaccga
     3841 aaaaatcggc aggtgcggct ggccgaacag ggtttcgccg gcgcggtaga acggcaggag
     3901 cagcagcagt tgcagcggat aagccaggta attcaccgcc tggatgatcg gctggttgag
     3961 ccgcag
//
LOCUS       DASZZC010000350         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_543872,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000350 DASZZC010000000
VERSION     DASZZC010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543872"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..>1621
                     /locus_tag="VHE37_07430"
     CDS             111..>1621
                     /locus_tag="VHE37_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT35396.1"
                     /translation="MHLEQPETERTEEALPYQPPGIQGLLRSAYRRHGRLRTNLFLGI
                     AGFFLLGLLYAEVCLRWLIWPALAAGLPADAETWSILHAAELRLVFAAMVITAAATFA
                     LYHLLLLPFEQARREFRRRPVASPALEKIMALSTLSDRRAALLDAALTDNEELRNQLE
                     QTSASARAQSLALEGLLGIATDAIVLVDREDRVIDLTASAATLVGWHRSSAAGRPFDA
                     VVQLFDQSKDHPLEYPLPSLAHRAQESGSLLPRLENAILLSQAREQQRVLASVCAVQG
                     SDGKPNGALVRLSIAEVKTARPSPVPGNAIAEPAQSMAPMVDAVTGLGLRDAFDRRLD
                     ELLEQARAQHVTHTLAFIRFDNLGTINDTHGFFAGEQLLWTAGSILREAVGSEGDGYR
                     VSTGRFAILFARPPRDALPVIERFRDQLSARELVWDNVRFATTVCVAVLPLDQTSEGR
                     KTLIDLAERELIEARKAGGNLVRSYAPDTTLVERRRIDGSWADWLLPRLDNGLA"
BASE COUNT          260 a          587 c          525 g          249 t
ORIGIN      
        1 cgagacacgc cgccgcctgc ggctggtcga ttcatacctg gagctgcgtc atctgtccgc
       61 agcccaaacc ctgctcgacg agatcgagca gcaccagcgg aggggatgac gtgcacctgg
      121 agcaacccga gaccgagcgc acggaagaag cgctgccgta ccagccgccc ggcatccagg
      181 gcctgttgcg ctcggcctac cggcggcatg gccgcctgcg taccaacctg ttcctcggca
      241 ttgccggatt tttcctgctg ggcctgctct acgccgaagt ctgtctgcgc tggctgatct
      301 ggccggcgct ggcagcgggc ctgccggccg acgccgagac ctggtcgatc ctgcatgccg
      361 cagaactgcg cctggtgttc gccgccatgg tcatcaccgc cgccgccacc ttcgcgctct
      421 accacctgct gctgctgcct tttgagcagg cccgccgcga gttccggcgc cgcccggtgg
      481 cgtcgccagc gctggaaaag atcatggcgc tgtccacgct cagcgaccgc cgcgccgcgc
      541 tgctcgacgc agcgctcacc gacaacgagg agttgcgcaa ccagcttgag cagaccagcg
      601 cgtcggcacg ggcacagtca cttgcgctcg aaggattgct tggcattgcc accgacgcca
      661 tcgtcctggt cgaccgcgag gaccgcgtga tcgacctcac cgccagcgcc gcgacgctgg
      721 tcggctggca tcgcagcagc gccgctgggc gtccgttcga cgccgtggtg cagctgttcg
      781 accagagcaa ggatcacccg ctggaatacc cgctgccgag cctggcgcat cgcgcgcagg
      841 agtccggctc gctgctgccc cggctggaga acgcgatcct gctcagccag gcacgcgaac
      901 agcaacgggt actcgccagt gtctgcgcgg tccagggcag cgacggcaag cccaacggcg
      961 cgctggtgcg cctgtccatt gcggaggtca agacggcgcg tccaagcccg gtgccgggca
     1021 acgccatcgc cgagccggcg cagtccatgg caccgatggt ggacgccgtc accgggctcg
     1081 gcctgcgcga tgcctttgat cgccggctcg acgaattgct cgagcaggca cgcgcacagc
     1141 acgtgacgca tacgctggcc ttcatccgct tcgacaacct gggaaccatc aacgataccc
     1201 acggcttctt cgctggcgaa cagctgttgt ggaccgccgg ctccatcctg cgcgaagccg
     1261 tcggcagcga aggcgacggc taccgcgtgt ccaccggccg cttcgccatc ctgttcgccc
     1321 ggccaccgcg cgatgcattg ccggtcatcg aacgcttccg cgaccaactg tccgcgcgcg
     1381 aactggtctg ggacaacgtg cgctttgcca ccacggtctg cgtggccgtg ctgccgctgg
     1441 accagaccag cgaagggcgc aagacgctga tcgacctggc cgagcgcgaa ctgatcgagg
     1501 cgcgcaaagc cggcggcaac ctggtgcgaa gctacgcgcc cgacaccacg ctggtggaac
     1561 gccgccgcat cgacggcagc tgggccgact ggctgctgcc gcggctggac aatggtttgg
     1621 c
//
LOCUS       DASZZC010000351         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_158453,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000351 DASZZC010000000
VERSION     DASZZC010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158453"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..682)
                     /locus_tag="VHE37_07435"
     CDS             complement(182..682)
                     /locus_tag="VHE37_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HVT35397.1"
                     /translation="MPTMYQASVPVFIRMLGNIRNVLQKGAAHAQAKKFEPSVLVQAR
                     LAPDMFALARQIQIATDMAKGCAARLAGVELPKYEDNETTIDELVARIDKTVAFLKTF
                     KPQQIDGSEGKAITLQMRTGERKFEGQPYLIDFVLPNFYFHLSTAYNILRHNGVEIGK
                     MDFLGG"
     gene            817..1053
                     /locus_tag="VHE37_07440"
     CDS             817..1053
                     /locus_tag="VHE37_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35398.1"
                     /translation="MKSASAAAAGLVLVLAGCAGNPPANQAADSHCIRDTGTRIPHEG
                     CTSQPGRSYTHDDIERTGAVDTGEAIRKLDPSVR"
     gene            complement(1050..2066)
                     /locus_tag="VHE37_07445"
     CDS             complement(1050..2066)
                     /locus_tag="VHE37_07445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT35399.1"
                     /translation="MDFERGSLFTRSSYLDPLEHCGCASPAAGWTPAHVETAAGRAPA
                     YLKMHSWGEFVFDFALARAYEQHGLRYYPKLVCSVPFTPVPGPRLLAADHTGRRKLAD
                     ALIGRARERKCSGAHVLFCTKDEADLLADAGWLTRVQPRYLWRNGGYADFETFLSTLN
                     SKQRKNIRRERRGIVESGLDIRWQSAEELDARDWPQLFRLYASTYAMRGQDAYLNLDC
                     LRQWGENFPRALRFCIARDRGEPVAMAFFFEEGDALYGRHWGASVQLSGLHFELCYYR
                     GIEWCIERGLQLFDAGVQGEHKLARGFAEELAHSAHWFAHDALRAPIRAWFEQERRAL
                     AAVS"
     gene            2136..2756
                     /gene="msrA"
                     /locus_tag="VHE37_07450"
     CDS             2136..2756
                     /gene="msrA"
                     /locus_tag="VHE37_07450"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HVT35400.1"
                     /translation="MALFGSSLKISPKDFPDAKDDLPRASAPGRALAVLAGGCFWCVE
                     AVYRELDGVLDVVNGYSGGSAETADYKTVCTGTTEHAEVVQIAYDPSRISYGKLLKIF
                     FSVAHDPTQLNRQGNDIGTQYRSAIFYADDAQKRVAENYIRQLDAAGVYSKPIVTRLE
                     KLADFFEAEGYHQNYAALHPNEGYIAGVALPKVDKLREYFGESLKR"
     gene            complement(2804..3451)
                     /locus_tag="VHE37_07455"
     CDS             complement(2804..3451)
                     /locus_tag="VHE37_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT35401.1"
                     /translation="MKLYAHPFSSYCQKVLIALYENRIDFEYRVLGGDAAIAAEHARL
                     WPLRRMPLLDDAGRVVIESSIIIEYLQLHHPGPVRLIPQGPDAALQVRTLDRYFDQYV
                     MTPMQKIVLDHLRPAAERDAYGVAQAHALLEESYAWLETQLTSTWAAGEEFTLADCAA
                     APSLFYADWVHPMAESMPKLRAYRARLLARPSFARAVDEARPFRQFFPPGAPDRD"
BASE COUNT          631 a         1162 c         1139 g          555 t
ORIGIN      
        1 tcgtgcagca gatctccacg ccgccgccgc cgcagggccc gcgccgctaa caagccgttt
       61 gcgaggaaaa aggccctacc gcactgccgt tctgcgtgtt ataaaatatt gttttaaaat
      121 aaaaacagac aatactttat aacgccgcaa gacggggtgc ggtgcaggca aaagcggcgc
      181 ctcagccgcc gaggaaatcc atcttgccga tctccacgcc gttgtggcgc aggatgttgt
      241 aggccgtgct caggtggaaa tagaagttgg gcagcacgaa gtcgatcagg tagggctggc
      301 cctcgaactt gcgctcgccg gtgcgcatct gcagcgtgat cgccttgcct tcgctgccgt
      361 cgatctgctg cggcttgaag gtcttgagga aggccaccgt cttgtcgatg cgcgcaacca
      421 gttcgtcgat ggtcgtctcg ttgtcctcgt acttgggcaa ctccacgccg gccagccgcg
      481 ccgcgcagcc cttggccatg tcggtggcaa tctggatctg gcgggccagc gcgaacatgt
      541 ccggcgccag gcgcgcctgc accagcacgc tgggctcgaa cttcttcgcc tgcgcgtgcg
      601 ccgcgccctt ctgcagcacg ttgcggatgt tgccgagcat gcggatgaac accggcaccg
      661 aggcctgata catggtgggc atgggtcact ccggttgggg ttgaggcggg cgacgatacg
      721 ccagcgcggc cggcgccgcc agcgcgcgcg gcgttcatgc gtggttcagc cgcacgcgcg
      781 gcataatgca ggccgttgca ccgcaggagg ctcgccatga aatccgcatc cgcagctgcc
      841 gccggcctgg tcctggtcct cgccggctgc gcaggcaatc cgcccgcgaa ccaggccgcc
      901 gacagccatt gcatccgcga taccggcacg cgcatcccgc acgagggctg caccagccag
      961 ccgggccgca gctacacgca cgacgacatc gagcgcaccg gtgcggtcga taccggcgag
     1021 gccatccgca agctcgaccc cagcgtacgt tagctgactg cggcaagcgc gcgacgttcc
     1081 tgctcgaacc acgcgcggat cggtgcgcgc agcgcgtcgt gcgcaaacca gtgcgcggag
     1141 tgcgcgagtt cttcggcgaa accgcgcgcc agcttgtgct cgccctgcac gccggcgtcg
     1201 aacagctgca atccgcgctc gatgcaccac tcgatgccgc ggtaatagca cagctcgaag
     1261 tgcaggccgc tgagttgcac gctggcgccc cagtggcggc cgtagagcgc atcgccttcc
     1321 tcgaagaaga acgccatcgc caccggctcg ccgcggtcgc gcgcgatgca gaagcgcaat
     1381 gcgcgaggaa aattctcgcc ccactggcgc aggcaatcga gattgagata ggcgtcctgc
     1441 ccgcgcatgg cataagtgga ggcatagagc cggaacagct gcggccagtc gcgcgcgtcg
     1501 agctcttccg cactctgcca gcggatgtcg aggccggatt ccacgatgcc gcgccgctca
     1561 cggcggatgt tcttgcgctg cttcgaattg agcgtggaga gaaacgtctc gaagtccgcg
     1621 tagccgccat tgcgccacag gtagcgcggt tgcacgcgcg tgagccagcc ggcgtccgcc
     1681 agcaagtcag cctcgtcttt cgtgcagaac agcacgtgcg cgccggagca cttgcgctcc
     1741 cgcgcgcgcc cgatcagcgc atcggccagc ttgcgccggc cggtgtggtc ggctgccagc
     1801 aggcgtggtc ccggcaccgg cgtgaacggc acgctgcaca ccagcttggg gtagtagcgc
     1861 aggccgtgct gctcataggc gcgcgccagc gcgaagtcga acacgaactc gccccaggaa
     1921 tgcatcttca ggtaagccgg cgcgcgcccc gcggcagttt ccacgtgcgc cggcgtccag
     1981 ccggcggctg gcgacgcgca gccgcagtgc tcgagcggat cgagataact ggaacgggtg
     2041 aacaagctgc cgcgttcaaa atccacgaga atgcgctgca accatttgcg ctgcggtcat
     2101 gtcatagggc cgcgcgaaga taacaggagt tcaacatggc cttgttcggc agcagcctga
     2161 agatcagccc gaaagatttt cccgatgcca aagacgacct gccgcgcgca tccgcacccg
     2221 gccgcgcgct ggcggtgctt gccggcggct gcttctggtg cgtggaggcg gtttaccgcg
     2281 aactcgacgg cgtgctcgac gtggtcaacg gctattccgg cggcagtgcc gagaccgccg
     2341 actacaagac cgtgtgcacc ggcaccaccg agcatgccga ggtggtgcag attgcctatg
     2401 acccgtcgcg catcagctac ggcaagctgc tgaagatatt tttctccgtg gcgcacgatc
     2461 ccacgcaact caaccgccag ggcaacgaca tcggcacgca gtaccgctcg gccatcttct
     2521 acgccgacga cgcgcagaag cgcgtcgcgg aaaactacat ccgccagctc gacgcggccg
     2581 gcgtgtacag caagcccatc gtcacgcggc tggaaaagct ggccgatttc ttcgaggccg
     2641 agggctacca ccagaactac gcggcgctgc accccaacga gggttacatc gccggcgtgg
     2701 ccctgcccaa ggtggacaag ctgcgagaat atttcggcga aagcctgaaa cggtagcgct
     2761 cggccgggcg gacacggcga ccggcttcat cgccgcgcgc tgctcagtcg cggtccggcg
     2821 cgcccggcgg aaagaactgg cggaacggcc gcgcctcgtc caccgcacgg gcgaatgacg
     2881 ggcgcgccag caggcgcgcg cgataagcgc gcagcttcgg catcgattcc gccatcggat
     2941 gtacccagtc ggcgtagaac agtgacggtg ccgcggcgca gtcggccaac gtgaattcct
     3001 cacccgcggc ccaggtcgag gtcagctgcg tttccagcca cgcataggac tcttcgagca
     3061 gcgcatgcgc ctgtgccacg ccgtatgcgt cgcgctcagc ggccggacgc aggtgatcga
     3121 gcacgatctt ctgcatcggc gtcatcacgt actggtcgaa gtagcggtcc agcgtgcgca
     3181 cctgcagtgc cgcgtcgggc ccttgcggga tcaggcgcac gggtccggga tgatgcagct
     3241 gcaggtactc gatgatgatg ctcgactcga tcaccacgcg gccggcgtca tccagcagcg
     3301 gcatgcgcct gagcggccac aggcgtgcgt gctcggcggc gatggcggca tctccgccca
     3361 gcacgcgata ttcgaaatcg atgcggttct cgtacagcgc gatcagcacc ttctggcaat
     3421 acgaggaaaa cgggtgggca tagagcttca tgcggaggtt ccggtggtgg acggcagcga
     3481 ccgcgcc
//
LOCUS       DASZZC010000352         7140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_282500,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000352 DASZZC010000000
VERSION     DASZZC010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282500"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..826)
                     /locus_tag="VHE37_07460"
     CDS             complement(14..826)
                     /locus_tag="VHE37_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HVT35402.1"
                     /translation="MYKPVIPWIGGKRRLAPALLPLFPEHTCYVEPFCGAAALYFLKE
                     PSEVEVLNDVNGDLVNLYRVIKHHLEEFVRQFKWALTSRQVYKWQQQTPVETLTDIQR
                     AARFYYLQRLAFGGRVDGQSFGTATTAPPPRLNLLRLEEELSAAHLRLDGTYIEHLPW
                     QECVKRYDRPHSLFYCDPPYWQTEGYGVEFGLDQYELMARQAREMAGTMIISVNDHPD
                     MRRAFEGLPMREIGINYTVGGGAGVERQELIIGNWRGGWPEPRPLTLQGGLW"
     gene            complement(989..1555)
                     /locus_tag="VHE37_07465"
     CDS             complement(989..1555)
                     /locus_tag="VHE37_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35403.1"
                     /translation="MDRINSANMAAGRKFQDADPGSGRQATFFNAAFFNSVQEELAGI
                     VETADLELDPDDNAQVLKALKLLMYPVGSIYITSDPDFLPETYFGGTWSKVAVGRVPV
                     GLDAGQTEFDTVGKQGGEKTHTLTVSELPSLQFSIPIGNANDTALGAAEGQSSNSGSL
                     NTNSLGGGQPHNNLSPYEVIGNIWKRTA"
     gene            complement(1555..2097)
                     /locus_tag="VHE37_07470"
     CDS             complement(1555..2097)
                     /locus_tag="VHE37_07470"
                     /inference="COORDINATES: protein motif:HMM:NF021583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HVT35404.1"
                     /translation="MAITADSYARQLRQLLPPGQLWARLAGGDDWRNLLKALGASLMR
                     AHKRIAAMPREADPRTASARLPDWETSVGLPDTCIPGGGSLQQRRNAVVARLRSTGGA
                     SPAYFIGLAAGYGYTITITPLGPAYWRATAAEDTVVVNCTCNGGCDDQLEAYGNDQLE
                     CLLNRVKPAHTQLDIAYGGP"
     gene            complement(2088..3167)
                     /locus_tag="VHE37_07475"
     CDS             complement(2088..3167)
                     /locus_tag="VHE37_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HVT35405.1"
                     /translation="MSTDRPSLAELNDQVKSDMVARLDGATPTLRRSIFGAIAGALAG
                     ALNALYGYADNLAKQCNPATATGKFLELWASLWAIVRKLASAAAGDVVVTGIAGTPVP
                     AGSQLASELGVTYATDDDAVIDGGGSITVSVSALTAGIEGNLDAGATLSFVSPLTNVD
                     VDATVDTAGLAGGADIETDDALRSRMFQRIQNPPHGGNKADYESWARSVAGITRAWVF
                     PLYSGVGTVRVYVANDDYVGANTASAGDVANVQAYIDSVKPVGVTKPDTTNGFEAVAA
                     VKTPVAINLTISPDTPENRQAVADNLAEMFFRRAEPEGTVRLHWVQKAIITAGTVDDY
                     TLSAPSADSVAASGHICTLGTITWP"
     gene            complement(3180..3401)
                     /locus_tag="VHE37_07480"
     CDS             complement(3180..3401)
                     /locus_tag="VHE37_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35406.1"
                     /translation="MDRLQRFLQLAIVAAFVIVVVMVLSDFMPGLKSEATAGLASVCV
                     MEVIHSWRALIRKNFGSEPSDDAPPGKTS"
     gene            complement(3414..5585)
                     /locus_tag="VHE37_07485"
     CDS             complement(3414..5585)
                     /locus_tag="VHE37_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35407.1"
                     /translation="MNKHLSALLLLLASSCAFAQATYDGRNIKTATAPLTALQTQAAK
                     TVLCNSGGSSASPTACTAQTLGSMLGLEVNADSLGAAGDNTTDDSSIFTTLLGNPFGV
                     TSPGKVYRVKNLVNPGNQLDCRGSTLKAAAGASWVIELNGLGAQLINCTIQDDAGAGY
                     YTLTSTTLSGTASPGATSITVASATGMAANKPIFITLDCGMDFPTAIDPSYVSGTTIP
                     LLDPIPNCATAAAVASGGTGYANGNNLIETGGEGAPVTLSVTNTSGGVIQSGGLTILS
                     PGFQTSVPSNPVALQKGSGTGATANFTWAGAASGKAVRTAWGLVKVDKAKYALLDNLH
                     ITNAPVGMQFMDTGTGTAPSFHTIVDTVTNINDDSNLLAGIVLLTKTSQTTFHNVQLH
                     GVTSSPNFGVVGVYQECHSTGGTPCGGNYFTNLTALGYEIGQVGQSTQLAHYVNAVFD
                     TVQFYGGVYFNPVSLLFGGDTQFSFTTVDGSTGSTGVGLTLAGTGSDVVGDSLTLRKH
                     ICGIWIGSNVTKVAIDQDTEQLNTYLCGPNDFNQRDAYLGGAQFSRSLHANPGTLNGD
                     AVYTSGFASGSGAQIGSETSSSNSPFYLSSVGTGCVVVKPGNTTKVQHCPNYSLYTAR
                     IDFTGNTPGSLTGCGTSPSITGNNKAGRITIGTGGATACTFTFDNSPNWASQPICHAN
                     NSTTGAALGAKAPAGNLTSKLEIDGTMNAGDVVTYMCVGIQ"
     gene            complement(5588..6274)
                     /locus_tag="VHE37_07490"
     CDS             complement(5588..6274)
                     /locus_tag="VHE37_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35408.1"
                     /translation="MSKNAQVVLNNAADQINTVMKGLKAWWQGTDSGYVNNGVQDMPT
                     LPNLIQQIMSSALNPTGGVDTRTPLTGFTVAMQDLLSSLQLTPAGTLASGTVQLPNNP
                     LPGQLAEISTTKTISALTVVAQNVGHTVHGGAAAPLVADTAISFRFNADQQAWARVIS
                     GVRIKTDTFSTGGGGPYPLTYPPVPGVALMLLDGYAQPANPFTVTGSSLALVGLDTSR
                     FDTVTFIYTY"
     gene            complement(6267..6827)
                     /locus_tag="VHE37_07495"
     CDS             complement(6267..6827)
                     /locus_tag="VHE37_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HVT35409.1"
                     /translation="MTDFALEPTGNGNALDIVLFDGDAVADDSLRASVLVSLFSDRRA
                     TAEQLAEAGLSPDDRRGSWTDTFAADGDRQGSLLWLLARERQTDKTLRRAREYAEDAL
                     RWLVSDAIASSVTVTAAWIGDGFLDLGVSIESTGGTLKLRYALLWANSAIDPLAGPNR
                     IAGAAAQLAQQFDFIWYQQVPGVTLV"
     gene            complement(6824..>7140)
                     /locus_tag="VHE37_07500"
     CDS             complement(6824..>7140)
                     /locus_tag="VHE37_07500"
                     /inference="COORDINATES: protein motif:HMM:NF037093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35410.1"
                     /translation="EPGESASYNLWGDFIHLKQDGTVHLKARSKLLVEAPETEFTGNV
                     TIDGNLHINGDTSGDGTGHFDGALDSATSVSDPHRSMQDIHDAFNPHTHGASPGPSTS
                     IP"
BASE COUNT         1104 a         2228 c         2477 g         1331 t
ORIGIN      
        1 tggtgccaga ccgtcaccag agaccgccct gcagagtcag cgggcggggt tctggccagc
       61 caccacgcca gttgccgatg atcaactctt ggcgttccac accggctccg ccgcccaccg
      121 tgtagttgat gccaatctca cgcattggaa ggccctcgaa ggcgcgccgc atgtccgggt
      181 gatcgttgac gctgatgatc atcgtgccgg ccatctcgcg cgcctggcgg gccatcagct
      241 cgtactggtc aaggccaaat tcgacgccgt agccctcggt ttgccagtac ggcgggtcgc
      301 aatagaacag gctgtgcggc ctgtcgtatc gcttcacgca ctcctgccac ggcaggtgct
      361 cgatgtaggt gccatccaac cgcaggtggg cagctgagag ctcttcctcc aagcggagga
      421 gatttagacg tggcgggggt gctgtcgttg cggtcccaaa gctttgcccg tccacccgcc
      481 ccccgaaggc cagccgctgc aggtaataga agcgggctgc gcgctggata tcggtgagcg
      541 tttcgacggg ggtctgctgc tgccacttgt agacttggcg gctggttaaa gcccatttga
      601 actgccttac aaactcctcc aggtggtgtt tgatcacgcg atacagattg accaggtcgc
      661 cgttcacgtc attgagcacc tcgacctcgc tcggctcctt gaggaaatac agggcagccg
      721 cgccgcagaa aggctcgaca tagcaggtgt gctctgggaa gagcggcagg agggctggcg
      781 caaggcgccg cttgccgcct atccacggaa tgacgggttt gtacatatgg tgagctgctc
      841 cctttcgttg ttaggctcct cccgctccgt acggggcggg aagccctggg ctggctcacc
      901 tggttcacgc caggtgttct agcggcgccg tcgtgctcca acacgccggc gtcgcttccc
      961 tcttatggct tcggtccgac gtccttcatc atgcggttcg cttccagata ttgccgatca
     1021 cctcatatgg cgacaggttg ttgtgcggct ggccgccgcc caggctgttg gtgttcagcg
     1081 atccgctgtt gctcgactgg ccttcggcgg cgccgagcgc tgtgtcgttg gcatttccga
     1141 tcgggatgct gaactgcagg gacggcagct cgctgacggt gagtgtgtgc gtcttctcgc
     1201 cgccctgctt gcccacggtg tcgaactcag tctggccagc atcgaggccc accggcacgc
     1261 ggcccacggc caccttcgac caggtaccgc cgaagtaggt ctccggcagg aagtccgggt
     1321 cggaggtgat gtagatcgag ccgaccgggt acatcagcag cttgagcgcc tttagtacct
     1381 gcgcgttgtc gtccgggtcg agctccaggt ccgcggtctc gacgatgccg gccagttcct
     1441 cctggaccga gttgaaaaag gcagcattga agaacgtggc ctggcggccg gagccgggat
     1501 ccgcgtcctg gaacttgcgg ccggcggcca tattggcgga attgatgcgg tccattacgg
     1561 gcctccgtag gcgatatcga gttgggtgtg cgcgggcttc acgcggttga gcaggcattc
     1621 cagctggtcg ttgccatagg cctccagctg atcgtcgcag ccaccgttgc aagtgcagtt
     1681 caccaccacc gtgtcctcgg cggcggtggc gcgccagtac gccgggccca gcggcgtgat
     1741 ggtgatggtg tagccgtagc cggcggccag gccgatgaag taggccggcg acgcgccgcc
     1801 agtggagcgc aggcgcgcca ccacggcgtt gcgccgctgc tgcagcgagc cgccgccagg
     1861 gatgcaggta tccggcaggc ctaccgacgt ctcccagtcc ggcagacgcg cactggccgt
     1921 gcgcggatcg gcctcgcgcg gcatggcggc gatgcgcttg tgcgcgcgca tcagcgaggc
     1981 gcccagcgcc ttgagcaggt tgcgccagtc gtcgccgccg gccaggcgcg cccagagctg
     2041 gccgggcggc agcagctggc gcagctggcg ggcatagctg tcggcggtta tggccacgtg
     2101 atggtgccca gcgtgcagat gtgcccggaa gcggcgacgc tgtcggcgct cggcgcgctc
     2161 agcgtgtagt cgtccacggt gccggcggtg atgatcgcct tctgcaccca gtgcaggcgc
     2221 acggtgcctt ctggttcggc gcggcggaag aacatctcgg ccaggttgtc ggccaccgcc
     2281 tgccggttct ccggcgtgtc gggcgaaatg gtcaggttga tggccaccgg cgtcttgact
     2341 gcggccacgg cctcgaagcc gttggtggtg tcgggcttgg tcacgccgac cggcttcacc
     2401 gagtcgatgt aggcctgcac attggccacg tcgccggccg acgccgtgtt ggcgcccacg
     2461 tagtcgtcgt tggccacata cacgcgcacg gtgccgactc ccgagtacag cgggaacacc
     2521 cacgcgcgcg taatgcccgc gaccgagcgt gcccatgatt cgtagtcggc cttgttgccg
     2581 ccgtgcggcg ggttctggat gcgctggaac atgcgtgagc gcagtgcgtc gtccgtctcg
     2641 atgtcggcgc cgccggcgag tccggccgta tcgaccgtcg cgtcgacgtc cacgttggtc
     2701 agcgggctga cgaagctgag agtggcgcct gcatcgaggt tgccctcgat gcctgcagtc
     2761 agcgcgctca ccgaaaccgt gatgctgccg ccgccgtcga tcacggcgtc atcgtcagtg
     2821 gcataggtga cgcccagctc ggaggccagc tgcgagccgg ccggcaccgg tgtgccggca
     2881 atgccggtaa cgacgacgtc gccggcggcg gccgacgcga gcttgcgcac gatggcccac
     2941 aggctggccc acagctccag gaacttgccg gtggccgtgg ccgggttgca ctgcttggcc
     3001 aggttgtcgg cgtagccgta cagcgcgttc aacgcaccag ccagcgcgcc ggcgatcgcg
     3061 ccgaagatcg agcggcgcag cgtcggcgtc gcgccatcaa ggcgcgcgac catgtcggat
     3121 ttgacctggt cgttgagctc ggcgagcgag ggacggtcag tgctcatggg aattcttggt
     3181 caggaggtct tgcccggcgg cgcgtcatcg ctgggctcgc tgccgaagtt tttgcggatc
     3241 agcgcgcgcc aggaatgaat cacctccatc acgcagacgc tggccaggcc ggcggtcgct
     3301 tcgctcttga ggcccggcat gaaatcggac agcaccatca ccaccacaat gacgaatgcg
     3361 gcgacgatcg ccagttgcag gaatctctga agtcggtcca cggtgttctc cctttactga
     3421 atgcccacgc acatgtacgt cacgacgtcg ccggcattca tggtgccgtc gatctcaagc
     3481 ttggaagtca ggttgccggc cggggccttc gcgcccagcg ccgcgccggt ggtcgagtta
     3541 ttggcgtggc agatcggctg gctggcccag ttcggcgagt tgtcgaaagt gaaggtgcag
     3601 gcggttgcac cgccggtgcc gatggtgatg cggccggcct tgttgttgcc ggtgatcgat
     3661 gggctggtgc cgcagccggt gagcgagccg ggcgtgttgc cggtgaagtc gatgcgcgcg
     3721 gtgtagagcg aatagttcgg gcagtgctgc accttggtcg tgttgcccgg cttaaccacc
     3781 acacagccag tgcccaccga ggacaggtag aacggcgagt tgctgctgct ggtctcggag
     3841 ccgatctgcg cgccgctgcc cgaggcgaat cccgacgtgt acaccgcgtc gccgttgagc
     3901 gtgccggggt tggcgtgcag tgaccgcgag aactgcgcgc ctcccagata ggcgtcgcgc
     3961 tggttgaagt cgttggggcc gcagagatag gtgttgagct gctcggtgtc ctggtcgatc
     4021 gccaccttgg tgacgttgct accgatccag attccgcaga tgtgtttgcg cagggtgagg
     4081 ctgtcgccga ccacatcgct gccggtacca gcgagcgtga ggcctacgcc agtggagcca
     4141 gtcgagccgt ccaccgtggt gaagctgaac tgtgtgtccc ctccgaacag caggctgacg
     4201 ggattgaagt acacaccgcc atagaactga accgtgtcga atacggcatt aacgtagtgc
     4261 gccagctgag tgctctggcc cacctggccg atctcataac caagcgcggt caggttggtg
     4321 aaatagttgc ccccgcaggg cgtgccgcct gtcgagtggc attcctgata gacgcccacc
     4381 acgccgaaat ttggcgacga agtcacgcca tgcagctgca cgttatggaa ggtggtctgt
     4441 gacgtcttgg tcagcagcac gatgccggcg agcaggttgc tgtcgtcatt gatgttggtg
     4501 accgtgtcga cgatcgtgtg aaaactgggc gcggtccccg tgcccgtatc catgaactgc
     4561 atgcccaccg gcgcattggt gatgtgcagg ttgtcgagca gtgcgtactt ggccttgtcg
     4621 actttcacca agccccaggc ggtgcggacg gccttgccgc tggccgcacc cgcccaggtg
     4681 aaattcgccg tcgcgccggt gcccgaaccc ttctgcagcg cgacggggtt gctcggtacc
     4741 gaagtctgga agccgggcga gagaatcgtc agccctcccg actggatgac gccgccgctg
     4801 gtgttggtga cgctgagagt gaccggtgcg ccttcgccac cggtttcgat caggttgttg
     4861 ccattggcat agcccgtgcc gccggatgcc accgctgcgg cagtggcgca gttcgggatc
     4921 ggatcgagca gcgggatcgt ggtgcccgag acgtaagagg gatcgatcgc cgttgggaaa
     4981 tccatgccgc agtcgagcgt gatgaaaatc ggcttgtttg ccgccatgcc cgtggcgctg
     5041 gcgaccgtga tggacgttgc gccgggcgag gccgtgcccg atagcgtggt cgacgtcagc
     5101 gtgtaatagc cggcgccggc atcatcctgg atggtgcaat tgatcagctg tgcgccgagg
     5161 ccgttgagct caatgaccca gctggcgccg gcagccgcct tgagcgtgga tccacggcaa
     5221 tccagctggt tgcctggatt caccagattc ttgacgcggt agaccttgcc cggagacgtc
     5281 acaccgaacg ggttgccgag cagcgtggtg aagatgctgc tgtcgtcggt ggtgttgtcg
     5341 ccagcggcgc cgagcgagtc tgcattcacc tccaggccga gcatcgaccc gagcgtttgc
     5401 gctgtgcacg ccgtgggact ggcggaagag ccgccggagt tgcacagcac ggtcttcgcc
     5461 gcctgcgtct gcagcgcggt gagcggcgcg gtggccgtct tgatgttgcg gccgtcgtat
     5521 gtcgcctgcg cgaaggcgca cgagctggcc agcagtagca gaagcgcgga gaggtgtttg
     5581 ttcatgatca gtaggtgtag atgaaggtga cggtgtcgaa gcgcgaggtg tccaggccca
     5641 ccagcgccag cgagctgccg gtaacggtga acggattggc aggctgggcg tagccgtcga
     5701 gcagcatcag cgccacgccc ggcacgggcg gataggtcag cgggtacggg ccaccgccgc
     5761 cggtgctgaa ggtgtcggtc ttgatgcgca cgccggagat cacccgcgcc cacgcctgct
     5821 ggtccgcgtt gaagcggaag gagatggcgg tgtcggcaac cagcggtgcg gctgcgccgc
     5881 cgtgcacggt gtggccgacg ttctgcgcca ccaccgtgag cgcgctgatc gtcttggtgg
     5941 tgctgatctc ggccagctga cccggcagcg gattgttcgg cagctgcacg gtgccgctgg
     6001 ccagcgtgcc ggccggcgtc agctgcagcg agctgagcag atcctgcatg gcgacggtga
     6061 agcccgtcag cggcgtgcgc gtgtcgacgc cgccggtcgg gttgagcgcg ctcgacatga
     6121 tctgctggat caggttgggc agcgtgggca tgtcctgcac gccgttgttc acgtagccgc
     6181 tgtcggtgcc ctgccaccag gccttgaggc ccttcatcac ggtattgatc tgatcggccg
     6241 cgttgttgag cacgacctgc gcatttttag acaagggtca cccccggcac ctgctggtac
     6301 cagatgaagt cgaattgctg tgccagctgc gccgcggcgc cggcgatgcg gttgggcccg
     6361 gccagagggt cgatcgcgga gttggcccac agcagcgcgt agcgcagctt gagcgttccg
     6421 cccgtgctct cgatcgacac gccgagatcc aggaagccgt cgccgatcca ggcggccgtg
     6481 accgtaacgg agctggcgat cgcgtcgctc accagccagc gcagcgcgtc ctcggcgtac
     6541 tcgcgcgccc ggcgcagcgt cttgtcggtc tgccgctcgc gtgccagcaa ccacagcagc
     6601 gacccctggc gatcgccatc ggcggcgaac gtatcggtcc agctgccgcg gcggtcatcc
     6661 ggcgacaggc ccgcctcggc cagctgctcg gcggtggcgc ggcgatcgct gaagagcgac
     6721 accagcaccg aggcgcgcag gctgtcgtcg gccacggcgt cgccatcgaa cagcacgatg
     6781 tcgagcgcgt tgccgttgcc ggtgggttcg agcgcgaagt cggtcatggg atgctcgtgc
     6841 tcggtcccgg cgaggcgcca tgcgtgtgcg gattgaaggc gtcgtggatg tcctgcatgg
     6901 agcgatgcgg atcgctcacg ctggtcgccg aatcgagcgc cccgtcgaag tggccggtgc
     6961 cgtcgccgct ggtgtcgccg ttgatgtgca ggttgccgtc gatggtcacg ttgccggtga
     7021 actcggtttc cggcgcctcc acaagcagct ttgaccgggc cttcaaatgg accgtgccgt
     7081 cctgcttgag atgaatgaaa tcgccccaca ggttgtagct ggcggactca cccggctcca
//
LOCUS       DASZZC010000353         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_477440,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000353 DASZZC010000000
VERSION     DASZZC010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477440"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VHE37_07505"
     CDS             complement(<1..180)
                     /locus_tag="VHE37_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT35411.1"
                     /translation="MAGRVEGKVALVTGGSSGIGRGTAERLAAEGAFVVVTDIQDPLG
                     EEVVAGIVKAGGKAEY"
     gene            complement(504..1859)
                     /locus_tag="VHE37_07510"
     CDS             complement(504..1859)
                     /locus_tag="VHE37_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1247 family transposase"
                     /protein_id="HVT35412.1"
                     /translation="MDHPEGAGLQRADRVDFDPRVRLEFRGTQLSSDGGLLVMRELDD
                     ALGLSDLASAALRDTRSGKNTVHRLDGLFRQSVFGRLAGYEDVNDANRLACDPVMRQV
                     VGGRAVDAQAASASQMGRFETETLALAGNRAALADLNGQWIDRFHDRNGLKYIVLDMD
                     SSVSPTHGDQEGSAWNGHFDCSCYHPNFLFNQFGMLERCALRHGNVHSADGWRDVLDP
                     VIARYAERDLGGRFFRADAAYAIPAIYERLEEARFFYAIRLPANAVLKDKIAHRLTRP
                     VGRPSLTKVKRFFEEFEYQAASWDKERRVIAKIEWHPGELFPRVGFIVTNLPMEPDWV
                     VRFYNQRGTAEQHIKEGKYAFRWTRLSCRKFRDNEVRLQLHALAYNLATFLRCIELPE
                     AMADWSLTSLQLKLIKIGARVVRHARTITFQLAEVAVTGTMVRAILAAIRRLRAPPLC
                     A"
     gene            2109..>2318
                     /locus_tag="VHE37_07515"
     CDS             2109..>2318
                     /locus_tag="VHE37_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35413.1"
                     /translation="MIEGLVAAIVLAGAGTGREEHTQLSPKAACRLVAESRIPSIRTY
                     SIAGTYFADGVHGSTFDLPGCDVGLS"
BASE COUNT          419 a          740 c          730 g          429 t
ORIGIN      
        1 gtattcggcc ttgccgccgg ccttcacgat gccggcgacc acttcttcgc ccaggggatc
       61 ctggatgtcg gtgaccacga cgaacgcgcc ctcggcggcc agcctctcgg ccgtgccgcg
      121 gccaatgccg ctcgatccac cggtgaccag cgccaccttg ccttccacgc gacccgccat
      181 gacgtctccc ttgccgctgg ccagctgtca gcccagcggt cgcgagcgaa gcagccatgc
      241 cgccggggaa gggaagcggc gtggcccggg cggagccctc agcctgcatt ccccaagtgg
      301 cgtggcgttt gaggtgaaga tcgcctgtat ccgagcgcta gacaaggatt ttctgccgca
      361 agcggcacct gcggtcgcgc agactgctgg atctggacgg atcagaccgc gaagccgctg
      421 tttccttgcc caggggcggc gtttttcagc gcagcggaca gatctgtcca gccgctttcg
      481 ttcagtttga gcgtggatcg cgatcatgcg catagcggtg gcgctcgcaa tcggcggata
      541 gcggcgagga tggcgcgtac catcgtgccg gtgacagcga cctcggccag ctggaaggtg
      601 atggtgcggg cgtgacggac cacacgtgcc ccgatcttga tcagcttcag ttgcaggctg
      661 gtcaacgacc agtcggccat ggcctcgggc agctcgatgc agcgcaagaa ggtggccagg
      721 ttgtacgcca gggcgtgcag ttgcagccgc acctcattgt cgcggaactt ccggcacgac
      781 agccgcgtcc agcgaaaggc gtatttgccc tctttgatgt gctgctcggc ggtgccgcgc
      841 tggttgtaga accgcaccac ccagtccggc tccatcggca ggttggtgac gatgaagccg
      901 acacgcggga acagttcgcc cggatgccat tcgatcttgg cgatcacccg gcgttccttg
      961 tcccaggacg ccgcctgata ctcgaattcc tcgaagaacc gcttgacctt ggtcagtgac
     1021 ggccgcccga cagggcgcgt tagccgatgc gcgatcttgt ccttgaggac cgcgtttgcg
     1081 ggcagccgga tggcgtagaa gaaccgcgct tcttccaatc gctcatagat cgccgggatc
     1141 gcgtaggcag catcggcccg gaagaacctg ccaccaaggt cgcgctccgc gtagcgcgca
     1201 atgacggggt cgagaacatc acgccagcca tcggcgctgt ggacgttgcc atggcgcagg
     1261 gcgcagcgtt ccagcatccc gaactggttg aacagaaagt tggggtgata gcagctacag
     1321 tcgaaatggc cattccaggc ggacccttcc tggtcgccat gggtcgggct gaccgagctg
     1381 tccatgtcca gaacgatgta cttcagcccg ttacggtcat ggaaccggtc gatccattgc
     1441 ccgttcaggt cggccagcgc ggcacggttc ccggccagag ccagcgtctc ggtctcgaac
     1501 cgtcccatct gcgatgccga ggccgcttgt gcatcgaccg ctctgccgcc gacaacttgg
     1561 cgcatgaccg gatcgcaggc gagacggttg gcgtcgttga catcctcgta tccggccagc
     1621 cgcccaaaga ctgattgccg gaacaggccg tcgagccgat ggaccgtgtt cttgccagag
     1681 cgagtatcgc gcagcgccgc tgacgccaaa tcggacaacc cgagcgcgtc atcaagctcg
     1741 cgcatcacca gaaggccgcc gtcggaactg agctgcgtgc cgcgaaattc cagccgcacg
     1801 cgagggtcga aatccacccg atctgcccgt tgcaagcccg caccctctgg gtgatccatg
     1861 aaacgcgccc ctcgcagcct tcaacaccat gttttatata ggaaatataa tggtcaggac
     1921 agcgaaatca gcgccttact tgggaaatgt gggttaacag cactagcctg aatgcgtagt
     1981 accgctcccc accccgaaca gcccttggcg gggtccgctt gtaggccgcg tccattgcgc
     2041 ggcccgcgac ccgttgcggt ccttcctggt tcgttggatt ttgccgcctg cgaaggagga
     2101 cggctgaaat gattgaaggc cttgttgcgg cgattgtcct ggccggtgcg ggaaccggga
     2161 gagaggagca cactcagctc tcgcccaagg ctgcgtgccg actggtggcc gaaagcagga
     2221 taccgagcat tcggacttac tcgatagccg gaacgtactt cgcagatggc gtccacggct
     2281 ccacgtttga tctgccaggc tgcgacgttg gtctaagt
//
LOCUS       DASZZC010000354        12635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_158598,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000354 DASZZC010000000
VERSION     DASZZC010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158598"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VHE37_07520"
     CDS             <1..181
                     /locus_tag="VHE37_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35414.1"
                     /translation="SGRQFWFFSCRASLCPSQILFSDQTLANFLQHSCSDMKHSSGVA
                     GRCAAIVHKGFQVRP"
     gene            178..951
                     /locus_tag="VHE37_07525"
     CDS             178..951
                     /locus_tag="VHE37_07525"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HVT35415.1"
                     /translation="MSTRPAVLCISGHDPSGGAGIHADIESCAALGTHALSVITSHTV
                     QDTQDVRRVTPVPPILLAEQIEVLAADCEISAVKVGLVGDAEQIKPIAAALTRLRRPA
                     VIDPVLRAGGGRNLVGALLRAAMIEQLLPLADLVTPNLGEARVLANCAGADADTCAAR
                     LLELGVRNVLITAGDEPGDAVSNAWYTTAAAPVRYTWPRITDRFHGAGCTLASAIAAL
                     LAQGSNLADAIEQGQRYAQQSLARAYAVGHGRKIPGRIP"
     gene            948..1601
                     /gene="thiE"
                     /locus_tag="VHE37_07530"
     CDS             948..1601
                     /gene="thiE"
                     /locus_tag="VHE37_07530"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVT35416.1"
                     /translation="MKRLRGLYAITSQRLCADDILLLRSAEAALRGGAALLQYRDKWS
                     DAAARQRRARALRELCVDHGALFIVNDEVELALHSGAQGIHLGATDATLQQARERLPP
                     GSLVGISCSNSAARIEAAAAAGADYVAIGRFHPSSTKPDAPPATLDHLRDMRARYPDL
                     PICAIGGITPEHSGPLVAAGADMIAAVDGLFGGDDAESAARRYCAAMGSHTRVTAFH"
     gene            1634..2917
                     /gene="hemL"
                     /locus_tag="VHE37_07535"
     CDS             1634..2917
                     /gene="hemL"
                     /locus_tag="VHE37_07535"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HVT35417.1"
                     /translation="MTRSERLFERAQQSIPGGVNSPVRAFRAVGGHPRFIASAQGAYL
                     TDADGRRYIDYVGSWGPMILGHRPAAVEAALKAQLEIGVSYGAPTELEIEMAELLCAR
                     VPGLEQVRLCSSGTEATMSALRLARGCTGRDFIIKFEGCYHGHGDALLVKAGSGALTL
                     GVPTSPGVPAAFAQYTLTATYNDLDSVAALLKQHPGKVAAIIVEPVAGNMNCVPPAPG
                     FLAGLRELCDRDGALLIVDEVMTGFRVHPRGACGLYGLRADLVTLGKIIGGGLPVGAF
                     GGRRDLMQQLAPVGPVYQAGTLSGNPLAMAAGLATLRALDEGALYEQLETRTRELLEG
                     LRAAARAAGVPFTTAQVGSMFGLFFTDLEQVTSFADVMACDAARFKRFFHAMLDRGVY
                     LAPSAYEAGFVGAAHGAQEIRATLAAAGESFRQLT"
     gene            2934..4943
                     /locus_tag="VHE37_07540"
     CDS             2934..4943
                     /locus_tag="VHE37_07540"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVT35418.1"
                     /translation="MLEWLQTFTAWAQAHKELALALVFLVSFFDCLFVVGLFLVFAAV
                     FLFGTGALIAMGAFDFWPTILIVACGGVLGDTVSYFIGRHYGVALFKRPFFAKRPGLV
                     ERGRVFFGRHGGKGLVVAHNVGVLRPLLPAMAGAYGLSLPRFALAIVPAALSWALVYI
                     VPGVAVGASMGLAAEVTRRLAILIVGLGATLWLALWLTSLFVRLMQARAQQFINAMLE
                     LSRRNRTLGQFGPDLADPAQPETPALALFATVLVVIGGITLLLLWGFDTHAQPPAYDL
                     AVYQSLRSIEEPWATALAVFFSLLGEWPVYLPYAAAAAMMLTWMRSDRAVQHWLAALS
                     FGLVIMLGLSLLPNISNPLEYMGLASSAHFPRDLVMAAVIYGFTPVLLGGAREPGARV
                     AIYGAMFALLLLIMLARLYLGTLWLSVGLTAVLTTLLFVAALGLGYRQRGVEKVSLRR
                     FWPALVVLLLAATLHERMDFAARKLEHTPAPVLKSLDAQRWWERDWLKLRARRQDVAG
                     RDKQLLNLQWAGDLTAIRAAMLQQGWQDPEELSFATALRWLSLTSPIKDLPMLPRYHA
                     GRHEVLRLRRDIDDDHQYSLRLWPSGHVLDNGQNVWTGTLIQQEVRVVFKIFRYPSNE
                     DAYTPSLDALTLPLPGFETRRVMRQPAGFATLLLRPAEDSHVPEQ"
     gene            5063..5992
                     /gene="ftsY"
                     /locus_tag="VHE37_07545"
     CDS             5063..5992
                     /gene="ftsY"
                     /locus_tag="VHE37_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HVT35419.1"
                     /translation="MADKSGNLFTRLRAGLNTGDSWLTRDIGSFFTAGALRADALEEL
                     ETRLLAADAGVEATQWLTQRLNREINAGRVKNADQLRAALKTALLELLQPVAQPLVMP
                     DYIKPFVLFVAGVNGVGKTTTIGKLALRYKREGKSVLLAAGDTFRAAAVEQLKSWADR
                     VQVPILAQGAGADSASVIYDAVASAKARGVDVVIADTAGRLHTQNHLMEELRKIKRVV
                     QKHDPYAPHEVLLVVDATTGGNALNQALQFHEAIGLTGIAMTKLDGTAKGGILLAIAR
                     RLALPIRYVGLGEAAEDLEPFDAQAYVDALIAG"
     gene            6053..6745
                     /gene="ftsE"
                     /locus_tag="VHE37_07550"
     CDS             6053..6745
                     /gene="ftsE"
                     /locus_tag="VHE37_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HVT35420.1"
                     /translation="MVHLHKVSHRYPGGNDVLTDVSFTVAEGEMVFLTGASGAGKSTL
                     LKLIALLNKATRGQIVVNHQNLAGMKRSQIPQFRQQIGFIFQNFNLLYDRTVFDNVAL
                     PLVIRGHSHADVGRKVRAALDAVGLLDKETLLPIFLSGGEQQRVGIARAVVAKPALIL
                     ADEPTGNLDPATAREVMNLFARYNEAGVSVVVATHAIDLIQQMPDFRIINLERGQVTN
                     KQPTGHSERRRG"
     gene            6738..7688
                     /gene="ftsX"
                     /locus_tag="VHE37_07555"
     CDS             6738..7688
                     /gene="ftsX"
                     /locus_tag="VHE37_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HVT35421.1"
                     /translation="MANATAGNTRPSLVQRMGGEHARVFFFSLGKLWQNPIGTLLTAA
                     AIGITLALPAGLDVFLRNVSAVSYSWEGTLQASLFLKDSVTRERGLELTHEIQARPGV
                     AGAAYVSREQSLAEFRELSGFGEALDTLAENPLPAVIVVAPRREQTREQVSVLIDDLS
                     KLPEVELAKLDQKWLERLYQILDMLGRAVSILAFFLGLAVIVIVGNTIRLDIENRREE
                     IVVMKLIGAPDSFIRRPFLYTGFWYGLLGAFISLLIVEGTLVALMGPSQRLAQLYESS
                     YRLAGLSLKATLLLFAGGIFLGWAGALFTVSRHLDKIEPQ"
     gene            complement(7690..8106)
                     /locus_tag="VHE37_07560"
     CDS             complement(7690..8106)
                     /locus_tag="VHE37_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35422.1"
                     /translation="MNSRADRLHVAAMCLVAAGTIAYWTVYFTSGATQVRTDAVYIGF
                     EDAFPLADGWMTLCYLLAAWFLWRGDRRAVLWSLCAGSAMVFLGSMDLLFNIEQGHFS
                     HAPSAEMWAEIVIVAYCWWFGPYTIRRMWRHPLNAA"
     gene            8239..9099
                     /gene="rpoH"
                     /locus_tag="VHE37_07565"
     CDS             8239..9099
                     /gene="rpoH"
                     /locus_tag="VHE37_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HVT35423.1"
                     /translation="MTDTALALRQPSMLMPLAGSLDSYLAHVGRIPLLDAEEEKRLAE
                     RLHGHNDLKAAQQLVLSNLRFVVHIARGYLGYGLPLADLIQEGNIGLMKAVKRFDPKV
                     GVRLISFAVHWIKAEIHEFVLKNWRIVRIATTKAQRKLFFNLRSSKQRLGWLNQAEVE
                     TIARDLKVKPEEVMQMESRLNGADVSFSATPADGEEAYVPEDYLADGSNAYEDIENAQ
                     WEEEREDKMRTALVALDDRSRDILNRRWLADGDKAGLQELADEYGVSAERIRQIEVAA
                     MKKLRKAIET"
     gene            complement(9142..10056)
                     /gene="cyoE"
                     /locus_tag="VHE37_07570"
     CDS             complement(9142..10056)
                     /gene="cyoE"
                     /locus_tag="VHE37_07570"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVT35424.1"
                     /translation="MSTLTHEHRSLRLLAHEYYELCKPRVVMLIVFTAIVGMFLAVPG
                     LPPLDRLLWGTIGIALQAASAAAVNQLIDRNIDARMARTCGRPLVTGLLSVGESSAFA
                     AIIGVAGFALLYAKVNPLCAWLTLCTLVGYAGIYTMFLKRATPQNIVIGGAAGAAPPL
                     LGWVAVTGEIHPHALILFAIIFVWTPPHFWALAIDRRDDYARADIPMLPVTHGIEFTR
                     LQVLLYTVLLFVTTLMPYLTGMSGLLYLAGAAVLGAMFLRYALLLQFAPRPGLPMRTF
                     GFSIVYLMGIFSLLLIDHYLPALLSLAK"
     gene            complement(10058..11035)
                     /locus_tag="VHE37_07575"
     CDS             complement(10058..11035)
                     /locus_tag="VHE37_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HVT35425.1"
                     /translation="MVWFRRINFAAVVLCFVVVVFGAYVRLNNAGLGCPDWPGCYGQL
                     TVPEAAHEVAAAQQSYPQKPVEAPKAWKEMIHRYLATTLGSLIVLLALIAWWRRDLPL
                     PRGLALGVLGTVLLQGALGALTVLWQVNPLTVTAHLLTGLTTLLLLFWMWLSVSPAAP
                     AAAPKVLMPLATVALALLVLQIFLGGWTSSNYAALGCPDFPTCHGSWLPETDLHEAFT
                     LWRGLGVNYEGGVLDNRARVTIHLIHRIGALAVTLVLGWLALRLARAGRRPLAAAVIA
                     ALLLQLGIAISMVKLQFPLDLADAHNAGAALLLLALAAVNFAVRRPEAA"
     gene            complement(11111..11710)
                     /locus_tag="VHE37_07580"
     CDS             complement(11111..11710)
                     /locus_tag="VHE37_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35426.1"
                     /translation="MNDRTRAGRRQFLLVALLFAAPLVAAWLLYFVFPQWQPKARSNY
                     GEVISPARPVPALALYDAEGRPAAEALLHTRWTLLYLGGAQCAQACQDALLQTRQIRV
                     LLNQNRDRVQRVYLAPDAAALATARAALPLTLHQDLLIVAEKGAATSPARNFFQPVDA
                     DAVYLIDPLGNWAMTFHAGSDYKKVLKDLKYLLQLSHIG"
     gene            complement(11707..12414)
                     /locus_tag="VHE37_07585"
     CDS             complement(11707..12414)
                     /locus_tag="VHE37_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HVT35427.1"
                     /translation="MAAFVFRPPLWAIAATAAGCALFAALGTWQLHRGQAKQALIDQY
                     AAAPAAPLELRGDSAARDGELPEAAARGTYLPVRQLLLDNQSHDHQPGYHVWTPLRLE
                     RGGLLLVNRGWIPLAAARQPLPAPEGAQAVRGRWRSLPRAGMQLEQGACVPAHPGQVV
                     NYPDATQLACLLGEPVAAGELLLDAAAPGGYVRDWHAAFDEFPPQRHYAYAAQWFAFA
                     ATALAVFIRLNLKRVST"
BASE COUNT         2120 a         4252 c         4258 g         2005 t
ORIGIN      
        1 aagtggccga cagttctggt ttttttcctg ccgggcgagt ttatgtccaa gccagattct
       61 cttttcggat cagacccttg ccaatttctt acagcattct tgcagcgaca tgaagcattc
      121 atcaggggtt gcgggccgtt gcgccgccat tgtgcataag ggttttcagg tccgtccgtg
      181 agcacgcgtc ccgccgtact gtgcatttcc ggccacgacc cttccggcgg cgccggcatc
      241 cacgccgaca tcgagagctg cgcggcgctc ggcacgcacg cgctcagcgt catcacctcg
      301 cacacggtgc aggacaccca ggacgtgcgc cgcgtgacgc cggtgccgcc gatccttctg
      361 gcggaacaga tcgaagtgct cgcggcggac tgcgaaatct ccgcggtcaa ggtcggcctg
      421 gtcggcgatg ccgagcagat caagccgatc gccgctgcat tgacccggct gcgccggccg
      481 gcggtgatcg atccggtgct gcgcgccggc ggcggccgca acctggtcgg cgcgctgctg
      541 cgcgcggcca tgatcgagca gctgttgccg ctggccgacc tggtcacacc caacctgggc
      601 gaagcgcgcg tgttggccaa ctgcgccggt gcggatgccg acacctgcgc ggcgcggttg
      661 ctcgaactcg gcgtgcgcaa tgtattgatc accgccggcg acgaaccggg cgatgcggtc
      721 agcaacgcct ggtacaccac ggctgccgcg ccggtgcgtt acacctggcc gcggatcacc
      781 gatcgtttcc acggcgccgg ctgcacgctg gcgagcgcca tcgccgcgct gctggcccag
      841 ggaagtaatc tcgccgatgc catcgagcag gggcagcgct atgcgcagca gtcgctggcg
      901 cgcgcctatg ccgtcggcca tgggcgcaag atcccggggc ggattccttg aaacgcctgc
      961 gcggcctcta cgcgatcacc tcacagcgcc tgtgcgcgga cgacatcttg ttgctgcgat
     1021 cagcagaagc cgcgctgcgc ggcggtgcag ccctgctgca gtaccgcgac aagtggagtg
     1081 acgccgcagc gcgccagcgc cgggcccgcg cgctgcgcga actctgcgtc gaccacggcg
     1141 cgcttttcat cgtcaacgat gaggtggaac tggcactgca cagcggcgcg cagggcatcc
     1201 atctgggcgc aaccgacgca acgctgcagc aggcgcgcga aagattgccg cccggaagcc
     1261 tggtcggcat cagctgctcg aactcggccg cgcgcatcga agccgcggcg gcggctggtg
     1321 cggactatgt tgccatcggg cgcttccacc cgtcctcgac caaacccgac gcgccgccgg
     1381 ccacgctgga ccacctgcgc gacatgcgcg cgcgatatcc cgatctgccg atctgcgcca
     1441 tcggcggcat cacgcccgag cacagcggac cgctggtggc cgccggcgcc gacatgatcg
     1501 cggcggtcga tggcctgttc ggcggcgatg acgcggagtc tgctgcgcgc cgctactgcg
     1561 cggccatggg cagtcatacg cgggtgacgg cgttccatta agctcttcgc cccggccgat
     1621 tccagctcca cccatgaccc gatccgagcg cctgttcgag cgcgcgcagc agtccatccc
     1681 cggcggcgtg aactcgccgg tgcgcgcgtt ccgcgccgtg ggtggacatc cgcgcttcat
     1741 cgcgtccgcg cagggcgctt acctgaccga cgccgacggc cggcgctaca tcgactacgt
     1801 cggttcctgg gggccgatga tcctgggcca ccgccccgca gcggtcgaag ccgcgctgaa
     1861 ggcacagctg gagatcggcg tgtcgtacgg agcgccgacc gagctggaaa tcgaaatggc
     1921 cgaactgctg tgcgcgcgcg tgccaggcct ggagcaggtg cgcctgtgca gcagcggcac
     1981 cgaggccacc atgagcgcgc tgcggctggc gcgcggctgc accgggcgcg acttcatcat
     2041 caagttcgaa ggctgctacc acggccacgg cgatgccttg ctggtcaagg ccggcagcgg
     2101 cgcgctgacg ctgggcgtgc cgacctcgcc gggcgtgccc gccgccttcg cccagtacac
     2161 gctgacggcg acctacaacg acctggacag cgtggcggcc ctgctgaaac aacatccagg
     2221 caaggtggca gcgatcatcg tcgagccggt ggccggcaac atgaattgcg taccgccggc
     2281 gccgggcttc ctcgccggat tgcgcgagct gtgcgaccgc gacggcgcgc tgctgatcgt
     2341 cgacgaggta atgaccggct tccgcgtaca cccgcgcggt gcctgcgggc tgtacggcct
     2401 gcgcgcagac ctggtcacgc tgggcaagat catcggcggc ggcctgccgg tgggcgcgtt
     2461 cggcgggcgg cgcgacctga tgcagcagct ggcgccggtg ggcccggtgt accaggccgg
     2521 caccctgtcg ggcaatccgc tggcgatggc ggcaggcctg gcgacgctgc gcgcgctcga
     2581 cgaaggcgcg ctgtacgagc agctggaaac gcgcacgcgc gaattgctcg aaggtttgcg
     2641 tgctgctgcg cgcgcggcgg gcgtcccctt caccaccgca caggtcggca gcatgttcgg
     2701 gctgttcttc accgacctag aacaagtcac cagttttgcc gacgtgatgg cctgcgacgc
     2761 cgcgcgcttc aagcgcttct tccacgccat gctcgaccgc ggcgtgtacc tcgctccgtc
     2821 cgcctatgag gccggcttcg tcggcgccgc acatggtgcg caggaaatcc gcgccacgct
     2881 ggccgccgcc ggcgagagct tccggcagct cacctgagga gcctgccggc gccatgctgg
     2941 agtggctgca gaccttcacc gcgtgggcgc aggcgcacaa ggaactggcg ctggcgctgg
     3001 tgttcctggt cagcttcttc gactgcctgt tcgtggtcgg cctgttcctg gtgttcgccg
     3061 ccgtgttcct gttcggcacc ggcgcgctca tcgccatggg cgcattcgat ttctggccca
     3121 ccatcctgat cgtcgcctgc ggcggcgtgc tgggcgacac cgtcagctac ttcatcggcc
     3181 gccactacgg cgtggcactg ttcaagcggc cgttcttcgc caagcggccc gggctggtcg
     3241 agcgcggccg cgtgttcttc ggccgccatg gcggcaaggg cctggtggtg gcgcacaacg
     3301 tgggcgtgct gcgcccgctg ctgccggcga tggccggcgc ctacgggctg agcctgccgc
     3361 gttttgcgct ggccatcgtg ccggcggcgc tgtcatgggc gctggtgtac atcgtgcccg
     3421 gcgtcgcggt cggtgcgtcg atggggctgg ccgcggaagt cacgcggcgc ctggccatcc
     3481 tcatcgtcgg cctcggcgcc acgctgtggc tcgcgctgtg gctgacctcg ctgttcgtgc
     3541 gcctgatgca ggcgcgcgcg cagcagttca tcaacgccat gctcgaactg tcacggcgca
     3601 accgcacgct cggccagttc ggcccggacc tggccgatcc ggcacagccg gaaacgccgg
     3661 cgctggcact gttcgccacc gtgctggtgg tgatcggggg catcacgctg ctgctgctat
     3721 ggggcttcga tacgcacgcg cagccgccgg cctatgacct cgccgtgtac cagtcgctgc
     3781 gcagcatcga ggagccttgg gccaccgcgc tcgccgtgtt cttctcattg ctgggcgaat
     3841 ggccggtgta cctgccctac gcggcggcgg cggcgatgat gctcacctgg atgcgcagcg
     3901 accgcgcggt gcagcactgg ctggcggcgc tgtcgttcgg cctggtgatc atgctcggcc
     3961 tgtcgctgct gcccaacatc agcaatccgc tggagtacat ggggctggcc tcgagcgcgc
     4021 attttccgcg cgacttggtg atggccgcgg tgatctacgg cttcacgccg gtgctgctcg
     4081 gcggcgcgcg cgagccgggc gcgcgcgtgg ccatctacgg cgcgatgttc gcgctgctgc
     4141 tgctgatcat gctggcacgc ctgtacctgg gcacgctgtg gctgtcggtg ggcctgaccg
     4201 cggtgctgac cacgctgctg ttcgtcgccg cgctcgggct gggctaccgc cagcgcggcg
     4261 tggagaaggt atcgttgcgc cgcttctggc cggcactggt ggtgctgctg ctggccgcca
     4321 cgctgcacga gcgcatggac ttcgccgcgc gcaagctcga gcacacgccg gcaccggtgc
     4381 tgaagtcact cgacgcgcag cgctggtggg agcgcgactg gctcaagctg cgtgcgcggc
     4441 ggcaggatgt ggccggccgc gataagcaac tgctcaacct gcaatgggcc ggcgacctca
     4501 cggcgatccg cgccgccatg ctgcagcagg gctggcagga cccggaagag ctgtcgttcg
     4561 ccaccgcgct gcgctggctg tcgctgacct caccgatcaa ggacctgccg atgctgccgc
     4621 gctaccacgc cggccgccac gaggtgctgc gcctgcgccg cgatatcgac gacgaccacc
     4681 agtattcgct gcggctgtgg ccatccgggc atgtgctcga caacggacag aacgtatgga
     4741 ctggcacgct gatccagcag gaagtgcgcg tggtgttcaa gatcttccgc tatccgagca
     4801 acgaggacgc ctacaccccg tcgctcgatg cgctgaccct gccgctgccc ggcttcgaga
     4861 cccggcgtgt gatgcgccag cccgccggct tcgccacgct gctactgcgt ccggcagagg
     4921 acagccatgt gccggagcaa tgatttcatg gtctgaaatt gctgaaccgc atggccactt
     4981 ttaatgttat taattattaa ttaaatataa aattatatga tattttataa cacgccaacg
     5041 ccgggtgcgg tggaccttca aaatggctga caagagcggc aacctgttca cccgcctgcg
     5101 cgccggcctc aacaccggag actcctggct gacgcgcgac atcggcagct tcttcaccgc
     5161 cggcgcgctg cgcgccgacg cactggagga actggagacg cggctgctcg ccgccgacgc
     5221 cggcgtcgag gcaacgcagt ggctcacgca gcggctcaac cgcgagatca acgcggggcg
     5281 cgtgaagaac gccgatcaat tacgcgcggc gctgaagacc gcgctgctcg aactgctgca
     5341 gccggtggcg cagccgctgg tgatgccgga ttacatcaag ccgttcgtac tgttcgtggc
     5401 cggcgtcaac ggcgtgggca agaccaccac catcggcaag ctcgcgctgc gctacaagcg
     5461 cgagggcaag agcgtgctgc tggccgccgg cgacaccttc cgcgccgccg cggtcgagca
     5521 gctgaagagc tgggccgacc gcgtgcaggt accgatcctg gcgcagggcg cgggcgccga
     5581 ttccgccagc gtgatctacg acgcggtggc ctcggccaag gccaggggcg tcgacgtggt
     5641 catcgccgac accgccggcc gcctgcacac gcagaatcat ctgatggaag aactgcgcaa
     5701 gatcaagcgc gtggtgcaga agcacgatcc ctacgcgccg cacgaggtgc tgctggtggt
     5761 cgacgccacc accggcggca acgcgctgaa ccaggcgtta cagtttcacg aagccatcgg
     5821 gctgaccggc atcgccatga ccaagttgga tggaaccgcg aagggcggta tcctgctggc
     5881 catcgcgcgg cgccttgctc tgcccatccg ctatgtcggc ctgggcgagg ccgccgagga
     5941 tctggaacca ttcgatgcac aggcctacgt cgacgcgttg atcgccggct aggcgtttgg
     6001 ggaaacacgt gacgcgctac cgcggaaccg ccgcagctgc ctgatggccg acatcgtcca
     6061 cctgcacaag gtcagccacc gctatccggg cggcaacgac gtgctgaccg acgtcagctt
     6121 caccgtcgcc gagggcgaga tggtgttcct caccggcgca tccggcgccg gcaagtccac
     6181 gctgctcaag ctcatcgcgc tgctgaacaa ggccacgcgc gggcagatcg tggtcaatca
     6241 ccagaacctg gcgggcatga agcgctcgca gattccgcag ttccgccagc agatcggctt
     6301 catcttccag aatttcaacc tgctctacga ccgcaccgtg ttcgacaacg tggcgctgcc
     6361 gctggtgatc cgtggccaca gccacgccga cgtcgggcgc aaggtgcggg cggcgctcga
     6421 tgcggtcggc ctgctcgaca aggaaaccct gctgccgatc ttcctctccg gcggcgagca
     6481 gcagcgcgtg ggcatcgccc gcgccgtggt ggccaagccg gcgctgatcc tggccgatga
     6541 gcccaccggc aacctcgacc cggccaccgc gcgcgaggtg atgaacctgt tcgcgcgcta
     6601 caacgaggcc ggcgtgtcgg tggtggtggc gacgcacgcc atcgacctga tccagcagat
     6661 gccggatttc cgcatcatca acctcgagcg tgggcaggtc accaacaagc agcccaccgg
     6721 gcattcggag cgccgccgtg gctaacgcca ccgccggcaa cacgcggccc tcgctggtgc
     6781 agcgcatggg cggcgaacat gcgcgcgtgt tcttcttctc gctgggcaag ctgtggcaga
     6841 acccgatcgg cacgctgctc accgccgccg ccatcggcat cacgctggcg ctgccggccg
     6901 ggctcgacgt gttcctgcgc aacgtcagcg cggtctcgta cagctgggaa ggcacgctgc
     6961 aggcctcgct gttcctcaag gacagcgtga ccagggaacg cggcctggag ctgacgcacg
     7021 agatccaggc gcgtcccggc gtggccggcg cggcctacgt gtcgcgtgag cagtcgcttg
     7081 ctgaattccg cgagctgtcc ggcttcggcg aagcgctcga cacgctggcg gaaaatccgc
     7141 tgccggcggt gatcgtggtg gcgccgcggc gcgagcagac gcgcgagcag gtcagcgtgc
     7201 tgatcgacga cctgtccaag ctgcctgaag tggaactggc caagctcgac cagaaatggc
     7261 tcgagcgcct gtaccagatc ctcgacatgc tcggccgcgc ggtcagcatc ctcgccttct
     7321 tcctggggct ggcggtgatc gtcatcgtcg gcaacaccat ccgcctggac atcgaaaacc
     7381 gccgcgagga gatcgtggtg atgaagctga tcggcgcgcc cgacagcttc atccgccggc
     7441 cgttcctcta caccggcttc tggtacggct tgctcggcgc cttcatctcg ctgctgatcg
     7501 tggaaggcac gctggtcgcg ctgatgggtc cgagccagcg cctggcgcag ctgtacgaga
     7561 gcagctaccg gctggccggc ctgtcgctca aggccacgct gctgctgttc gctggcggca
     7621 tctttctcgg ctgggccggc gcgctgttca ccgtcagccg gcacctggac aagatcgagc
     7681 cgcagtagct caggccgcgt tgagcggatg tcgccacatc cggcggatgg tgtacggacc
     7741 gaaccaccag cagtacgcca cgatcacgat ctccgcccac atctcggcac tgggcgcgtg
     7801 gctgaagtgt ccctgctcga tgttgaacag caggtccatc gaaccgagga acaccatcgc
     7861 gctgccggcg cacaggctcc acagcaccgc gcggcgatca ccgcgccaga ggaaccacgc
     7921 ggccagcagg tagcacagcg tcatccagcc gtcggccagc ggaaacgcat cctcgaagcc
     7981 gatatacacc gcgtcggtgc gcacctgggt ggcaccggag gtgaaataga cggtccaata
     8041 ggcgatggtt ccggcagcga ccaggcacat ggcggcgacg tgcaggcggt cggctctgga
     8101 gttcatggat accctcttga agtgcttgat ttcaggccca tctaaggtac gggaatccgg
     8161 gcgggaccag gaactttttt accatttagc actccaagcg ctggattgct aaactattgc
     8221 tccggagggg tttttgccat gaccgataca gcacttgccc tgcgccagcc cagcatgctg
     8281 atgccgctgg ccggcagcct cgacagttac cttgcccatg tgggccgcat tcccctgctc
     8341 gatgcggagg aagagaagcg cctggccgag cgcctgcatg gccacaatga tctcaaggcc
     8401 gcgcagcagc tggtcttgtc caacctgcgc ttcgtggtgc atatcgcgcg cggctacctc
     8461 ggctatggcc tgccgctggc cgacctgatc caggaaggca atatcggcct gatgaaggcg
     8521 gtcaagcgct tcgatccgaa ggtcggcgtg cgcctgatct ccttcgcggt gcactggatc
     8581 aaggccgaga tccacgaatt cgtgctgaag aactggcgca tcgtgcgcat cgccaccacc
     8641 aaggcgcagc gcaagctgtt cttcaacctg cgctcctcca agcagcgcct gggctggctc
     8701 aaccaggcgg aagtggaaac catcgcgcgc gatctcaagg tcaagcccga ggaagtgatg
     8761 cagatggagt cgcgcctcaa cggcgccgat gtctccttca gcgccacgcc ggccgacggc
     8821 gaagaagcct acgtgccgga ggattacctg gccgacggca gcaacgccta cgaagatatc
     8881 gagaacgcgc agtgggaaga ggaacgcgag gacaagatgc gcaccgcgct ggtagcgctg
     8941 gacgaccgct cgcgcgacat cctcaatcgc cgctggctgg ccgacggcga caaggccggg
     9001 ctgcaggaac tggccgacga gtacggcgtg tcggccgagc gcatccgcca gatcgaggtg
     9061 gcagcgatga agaaactgcg caaggcgatc gaaacctgag cgataccgct gtcgaaaaag
     9121 cccgccgatg gcgggctttt tttattttgc gagcgacaaa agcgccggca ggtagtgatc
     9181 gatcagcagc aggctgaaga tgcccatcag gtagacgatg gaaaacccga acgtgcgcat
     9241 cggcaggccc ggccgcgggg cgaactgcag cagcagcgca tagcgcagga acatggcgcc
     9301 gagcaccgct gcgccggcca ggtagagcag gccgctcatg ccggtgagat acggcatcag
     9361 cgtggtcacg aacagcagca cggtgtagag cagcacctgc aggcgggtga actcgatgcc
     9421 gtgcgtgacc ggcagcatgg ggatgtcggc gcgcgcgtag tcgtcgcggc ggtcgatggc
     9481 cagcgcccag aaatgcggcg gcgtccacac gaagatgatg gcgaacagga tgagcgcgtg
     9541 cggatggatt tcgccggtca ccgccaccca gccgagcagc ggcggcgccg cgcccgccgc
     9601 gccgccgatg acgatgttct gcggcgtcgc acgcttgagg aacatggtgt agatgccggc
     9661 ataaccgacc agcgtgcaca gcgtgagcca ggcgcacagc ggattgacct tggcgtacag
     9721 cagggcaaat ccggcaacgc cgatgatcgc ggcaaacgcg ctcgactcgc ccaccgacag
     9781 caggccggtg accagcggcc gcccgcaggt gcgcgccatg cgcgcgtcga tgttgcggtc
     9841 gatcagctgg ttcaccgcgg cagccgaagc cgcctgcaat gcaatgccga tcgtgcccca
     9901 cagcagcctg tccaacggcg gcaatcccgg gacggcgagg aacatgccga cgatggcggt
     9961 gaacacgatc agcatcacca cccgcggctt gcacagctcg tagtactcgt gcgccagcag
    10021 gcgcaggctg cggtgctcgt gggtcagcgt gctcatctca agcggcctcg ggccggcgca
    10081 cggcgaaatt caccgcggcc agcgccagca gcaacaatgc tgcgccggca ttgtgcgcgt
    10141 cggccaggtc cagcgggaac tgcagcttga ccatggagat ggcaatgccc agctgcagca
    10201 gcagtgcggc gatcaccgcc gcggccagcg gcctgcgccc ggcgcgcgcc agtcgcagcg
    10261 ccagccagcc gagcaccagc gttaccgcca gcgcgccgat gcgatggatc aaatggatgg
    10321 tgacgcgtgc gcggttgtcg agcacgcctc cctcgtagtt cacgcccagc ccgcgccaca
    10381 gggtgaacgc ttcgtgaaga tcggtttccg gcagccagct tccatggcag gtcggaaagt
    10441 ccgggcaacc gagcgcggcg taattgctgc tggtccagcc gcccaggaag atctgcagca
    10501 ccagcagcgc gagcgccacg gtggccagcg gcatcagcac tttcggtgca gccgccggcg
    10561 cggccggaga gaccgacagc cacatccaga acagcagcag cagcgtggtg aggccggtga
    10621 gcaggtgcgc ggtcaccgtc agcgggttga cctgccacag cacggtgagc gcgccgagcg
    10681 cgccctgcag cagcacggtg cccagcacgc ccagtgccag tccacgcggc agcggcaggt
    10741 cgcgccgcca ccaggcgatg agcgccagca gcacgatcag cgaacccagc gtggtggcga
    10801 gatagcggtg aatcatctcc ttccatgcct tgggcgcttc tacgggcttc tgcggatagc
    10861 tctgctgcgc ggcggccacc tcgtgcgcag cttccggcac ggtgagctga ccatagcagc
    10921 ccggccagtc gggacagccg aggccggcat tgttcagacg aacgtaggcg ccgaacacca
    10981 cgaccacgaa gcacagcacg acggcggcga aattgatgcg gcggaaccag accatggaca
    11041 gcgtaccgat gaggggcgcg gattttatct cgaagcagga ggctgcgcgg cggcgacgta
    11101 tacgccgccc tcagccgatg tgcgacagct gcagaagata cttgaggtcc ttcagcacct
    11161 tcttgtagtc cgagccggca tggaaggtca tagcccagtt gcccagcggg tcgatcaggt
    11221 agaccgcatc ggcgtcgacc ggctggaaga aattccgcgc aggcgacgtg gccgcgcctt
    11281 tctccgcgac gatcagcagg tcctggtgca gcgtcaatgg cagcgcggcg cgcgcggtgg
    11341 ccagtgcagc ggcgtccggc gccaggtaca cgcgctgcac acggtcgcgg ttctggttga
    11401 gcaggacgcg gatctgccgc gtctgcagca gggcgtcctg gcaggcttgc gcgcactgcg
    11461 cgccgcccag gtagagcagc gtccagcgcg tatgcagcag tgcctcggca gcgggccggc
    11521 cctcggcatc gtacagggcg agcgccggca cgggccgcgc gggcgagatc acttcaccgt
    11581 agttgctgcg cgccttgggc tgccattgcg ggaagacgaa gtacagcagc caggcagcga
    11641 ccagcggcgc ggcgaacagc agcgcaacga ggaggaactg gcgccgtccg gcgcgggtgc
    11701 gatcgttcat gtcgaaacac gcttgagatt gagcctgatg aagacggcga gcgcagtggc
    11761 ggcgaaggcg aaccattgcg cggcataggc gtagtggcgc tgcggcggga actcgtcgaa
    11821 ggcggcgtgc cagtcacgca cgtagccccc cggcgcagcg gcgtcgagca gcagttcgcc
    11881 cgccgcgacg ggctcgccga gcaggcaggc gagttgcgtt gcatccgggt agttcaccac
    11941 ctggcccgga tgcgccggca cgcacgcacc ctgctcgagc tgcatgcccg cgcgcggcag
    12001 gctgcgccag cggccacgca ctgcctgcgc gccctcggga gcgggcagcg gctggcgcgc
    12061 ggcggccagc ggaatccagc cgcgattcac cagcagcagg ccgccgcgct caaggcgcaa
    12121 cggcgtccac acgtgatagc cgggttgatg gtcgtgcgac tggttgtcga gcaacagctg
    12181 gcgcaccggc agataggttc cgcgtgcggc ggcctccggc agttcgccat cccgcgccgc
    12241 gctgtcgccg cgcaactcga gcggcgccgc gggcgccgcg gcgtactggt cgatcagcgc
    12301 ctgcttggcc tgcccgcggt gcagctgcca ggtgccgagc gcagcaaaca atgcgcagcc
    12361 tgccgccgtc gcagcgatgg cccacagcgg cggccgaaag acgaatgcag ccatttcagg
    12421 tgctcaccgc atcgccggtt gatctgttat taaatatcat attttatcat atttaataga
    12481 taaataataa catcacatgg cagggtgcgg tggcggtctt tttcgcgcaa tggaagccgc
    12541 cgcagctatc atcggcgccc gcaaaccatc cgaccttcca tgctgaccaa gatcaccgtt
    12601 gtcgcactgc tgctggccat cgtcggcacg ctgat
//
LOCUS       DASZZC010000355         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1053744,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000355 DASZZC010000000
VERSION     DASZZC010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053744"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VHE37_07590"
     CDS             <1..286
                     /locus_tag="VHE37_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terpene utilization protein AtuA"
                     /protein_id="HVT35428.1"
                     /translation="NIGVIARKPEFLPHIRRALSVGAVKKYFAHVLEGGEGGKVERWE
                     LPGTHALNFLLYNCLGNGGAASLRTDPQGKCFAQMLLDFPIAIPKGLIHG"
     gene            279..968
                     /gene="can"
                     /locus_tag="VHE37_07595"
     CDS             279..968
                     /gene="can"
                     /locus_tag="VHE37_07595"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HVT35429.1"
                     /translation="MDELQRLLDNNEKWATESVKRDPDFFKRLVGQQNPQYLWIGCSD
                     SRVPANEILGLLPGEVFVHRNVANVVVHSDLNCMSVIQFAVDILKVKHIILCGHYGCS
                     GVHAAQHEKRVGMADFWLGHVRDVAGHYHHLIERESKDRHKDALLCELNVLEQALNVI
                     SSPVVLDAWNRGQELSVHGWIYSLKDGRLRHLNFSADRPQDIPALRRKAVDELLAARD
                     AGIARKKTGGA"
     gene            1068..>1839
                     /locus_tag="VHE37_07600"
     CDS             1068..>1839
                     /locus_tag="VHE37_07600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT35430.1"
                     /translation="MSVERDVMEYDVVIVGGGPSGLSAACRIKQVAAQAGKDITVCLV
                     EKGSEVGAHILSGAVFEPRALAELFPDYEAKGAPLGVPVKRDDVYFFMSPTSAIKMPN
                     LFVPATMHNEGNRIISLGNLCRWLNTQAQELGVDVIEGFAAQSPIIEDGVVKGITVGD
                     KGIDRHGKQTADYTPGPEIRAKYTILAEGARGHIGKQILHKFDLCKSADAQHWAIGIK
                     EIWDAPPGRHEPGLVVHGAGWPLSTFGSENPGGSFLYHA"
BASE COUNT          343 a          582 c          607 g          307 t
ORIGIN      
        1 caacatcggc gtcatcgcac gcaaaccgga attcctgccg cacatccggc gcgcgctgag
       61 cgtcggggcc gtgaagaaat atttcgccca tgtgctcgaa ggcggcgagg gcggcaaggt
      121 ggagcgctgg gaacttccgg gcactcatgc gctcaatttc ctgctgtata actgcctggg
      181 caacggcggt gcggccagcc tgcgcaccga tccgcaagga aaatgttttg cccagatgct
      241 cctcgatttt ccgattgcca tacccaaagg attgatccat ggatgagctg cagcggttgc
      301 tcgacaacaa cgagaagtgg gcgacggaat cggtcaagcg cgacccggat ttcttcaagc
      361 gcctggtcgg gcagcagaac ccgcagtacc tgtggatcgg ctgctccgat tcgcgcgtgc
      421 cggccaacga gattctcggc ctgctgccgg gcgaggtgtt cgtgcaccgc aacgtggcca
      481 atgtcgtcgt gcattccgac ctcaactgca tgtcggtgat ccagttcgcg gtcgacatac
      541 tcaaggtgaa gcacatcatc ctgtgcggtc actacggctg cagcggtgtg cacgcggccc
      601 agcacgagaa gcgcgtgggc atggccgatt tctggctggg ccacgtgcgc gatgtggccg
      661 gccactacca ccacctgatc gagcgtgagt ccaaggaccg ccacaaggat gcgctgctgt
      721 gcgagctcaa cgtgctggag caggcgctca acgtcatctc cagtccggtg gtgctcgacg
      781 cctggaaccg cggacaggaa ctgtcggtgc acggctggat ctacagcctc aaggacggcc
      841 gcctgcgcca cctgaatttc tctgccgacc ggccgcagga cattccggcg ctgcggcgca
      901 aggcggtgga tgagttgctg gcggcacgcg acgccggcat cgcacgcaag aagaccggcg
      961 gcgcttgacc gccgcgccag cgtgcgctgg cgccaaaacg ggttttccct cagccgattc
     1021 gggtaaaatc gcggctcttt ttcgcccagc tttcccggaa aaacggcatg agcgttgaac
     1081 gcgacgtgat ggaatacgac gtggtaatcg tcggcggcgg tccgtcgggc ctgtctgcgg
     1141 cgtgccgcat caagcaggtg gcggcgcagg ccggcaagga catcaccgtc tgcctggtgg
     1201 agaagggctc cgaggttggc gcgcacatcc tctccggggc ggtgttcgag ccgcgcgcgc
     1261 tggccgaact gttcccggac tacgaggcca agggcgcgcc gctgggcgtg ccggtcaagc
     1321 gcgacgacgt gtatttcttc atgtcgccga ccagcgccat caagatgccc aacctgttcg
     1381 tgcccgcaac catgcacaac gagggcaacc gcatcatctc gctgggcaac ctgtgccgct
     1441 ggctgaatac gcaggcgcag gaactgggtg tcgacgtcat cgaaggattt gccgcgcagt
     1501 cgccgatcat cgaggacggc gtggtcaagg gcatcacggt gggcgacaag ggcatcgacc
     1561 gccacggcaa gcagacggcc gactacacgc cgggccccga gattcgcgcg aagtacacca
     1621 tcctcgccga gggcgcacgc ggccatatcg gcaagcagat cctgcacaaa ttcgacctgt
     1681 gcaagagcgc cgacgcgcag cactgggcga tcggcatcaa ggaaatctgg gacgcgccgc
     1741 cgggcaggca cgagccgggg ctggtggtgc atggcgcggg ctggccgctg tccaccttcg
     1801 gcagcgagaa ccctggcggc tcgttcctgt accacgccg
//
LOCUS       DASZZC010000356         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_477848,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000356 DASZZC010000000
VERSION     DASZZC010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477848"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VHE37_07605"
     CDS             complement(<1..285)
                     /locus_tag="VHE37_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35431.1"
                     /translation="MKNQGAVTAQHAWMRASAALACAVAACAHVPDAHERALIDARAK
                     MHAAGAALVADGCVMRASGVEQYYLIGDSRRVASIAAKAGGALLRQQNVPL"
     gene            complement(369..2162)
                     /locus_tag="VHE37_07610"
     CDS             complement(369..2162)
                     /locus_tag="VHE37_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVT35432.1"
                     /translation="MGKYVPPMRDMKFVLQEVLDVVTEYRAMPKHAELDIDTMSSIVE
                     EAGKFVANEIFPLNQSGDQEGCTYHGDGVVTTPKGFKEAYRKYIEAGWPALSSATEYG
                     GQGLPELTNSVFMEMMNSGNQAWTMYPGLSHGAYAALLAHGTEELKKLYLPRLIDGTW
                     TGTMCLTEAHCGTDLGLLKTRAEPQADGSYSISGTKIFISAGEHDMSDNIVHLVLARL
                     PDAPAGSKGISLFVVPKFIPDAAGKPGKRNGVKCGSIEHKMGIHGNATCVINLDSAQG
                     WMVGEPNKGLAAMFVMMNGARLGVGMQSLGLAEVAYQNSLAYAKDRLQMRSLTGVKAK
                     DKPADPIIVHPDVRRMLLTQKAYVEGARAFTYWIAMNIDKQHDHPDEKVRKDADDLVS
                     LLTPVVKGFITDNGYESTTLAMQVLGGHGYIAEWGMEQYVRDARINMIYEGTNTVQAL
                     DLLGRKVLGDMGAKLMKFGKIISDFIKAQGDRPEMAEFTKPLAELATELQKLTAEIGQ
                     KAMKNPDEAGAAATPYLRIVGHLVFGYFWARMARVALDKQSGGSASGFYAAKIATARF
                     YFDKLMPEAWAQIKIARSGSASLMAMPAENF"
     gene            2286..3551
                     /locus_tag="VHE37_07615"
     CDS             2286..3551
                     /locus_tag="VHE37_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HVT35433.1"
                     /translation="MNSPAPLAGPRRAALVFILVTVAIDILSFGIIIPVLPKLVVDFV
                     GSEADGARMFGWFSMAWATMQFIFSPLLGAISDRYGRRPVILISCFGLGVDFLVMALA
                     PNLAWLFAGRLVSGICAASFSTAGAYIADVTPAEKRATAFGMMGVAFGIGFVVGPFIG
                     GELGALSPRAPFWAAACLALVNACYGVFVLPESLPTERRGGFSWARANPLGSLKLLRS
                     HHELFGFAGVSFLANLAHVVLPSTTVLYMGYRYHWDARDVGRVLGLVGICSAIVQGGL
                     IRPAVKRFGERKAMLFGLACGTVGFMIYGLAPTGTWFLVAIPIMAFWGLAMPSLQSLM
                     TQRVSPTEQGQLQGANSALIGIAGMIGPGLFTQTFAHFIDPVGLQLPGAPYYIASLLL
                     FVGLILAWRITRYAAAAADVATAAAATES"
     gene            3666..4061
                     /locus_tag="VHE37_07620"
     CDS             3666..4061
                     /locus_tag="VHE37_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35434.1"
                     /translation="MKTKTITRPAVLGLLFSAALVSGAAFAQTAPTTTVTTSTTTTVP
                     AAEPGHPRVNEVDQRLANQQKRIDAGVASGKITAAQAARDEKRDANIEARAQADEAKH
                     GGHLTKKEQERLNGSLNKDSNKIHEQKTN"
     gene            4196..4543
                     /locus_tag="VHE37_07625"
     CDS             4196..4543
                     /locus_tag="VHE37_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35435.1"
                     /translation="MKLLHLALLLGALAAGPAWADKDMLGREIEAPKRHAETPPPALP
                     GLSGDVNPQEPQNSSEADAAAAKSPAPADAAAFEVQPDAAGKMQQQSRPKPPPVDHPP
                     EDAGHRKPEGQQR"
BASE COUNT          761 a         1608 c         1495 g          880 t
ORIGIN      
        1 gagcggcacg ttctgctggc gcagcagcgc gccaccggct ttcgccgcaa tcgatgccac
       61 gcggcgcgag tcgccgatca ggtagtactg ctccacgccg ctggcgcgca tcacgcagcc
      121 gtccgccacc agcgccgcgc cggcggcgtg catcttcgcg cgagcgtcga tcagcgcgcg
      181 ctcatgcgca tccggcacat gcgcgcaggc cgccacggcg cacgccagcg ccgcgctggc
      241 ccgcatccat gcgtgttgtg ccgtcaccgc accctggttt ttcatgttat taattattgt
      301 ctatatttat atatgtacaa tattacataa catgcaaaat ggcagtgcgg tgagcggttt
      361 ttccggcctc agaagttttc ggccggcatc gccatcagcg aagccgagcc gctgcgcgcg
      421 atcttgatct gcgcccatgc ctccggcatc agcttgtcga agtagaaccg ggcggtggcg
      481 atcttggccg cgtagaagcc gctggcgctg ccgccggact gcttgtccag cgccacgcgc
      541 gccatgcgtg cccagaaata gccaaacacc aggtggccga cgatgcgcag gtacggcgtc
      601 gccgccgcgc cggcttcgtc gggattcttc atcgctttct ggccgatttc cgcggtgagc
      661 ttctgcagtt cggtggccag ctcggccagc ggcttggtga actccgccat ttccgggcga
      721 tctccctgcg ccttgatgaa gtcagagatg atcttgccga acttcattag cttggcgccc
      781 atgtcgccca gcaccttgcg cccgagcaga tccagcgcct gaaccgtgtt ggtgccttcg
      841 tagatcatgt tgatgcgcgc gtcgcgcacg tactgctcca tgccccactc ggcgatgtag
      901 ccgtggccgc ccagcacctg catggccagc gtggtggact cataaccgtt gtcggtgatg
      961 aagcccttga ccaccggcgt cagcagcgag accaggtcgt cggcgtcctt gcgcaccttc
     1021 tcgtcggggt gatcgtgctg cttgtcgatg ttcatcgcga tccagtaggt gaacgcacgc
     1081 gcgccctcta catacgcttt ctgcgtgagc agcatgcggc gcacgtcggg gtgcacgatg
     1141 atcggatcgg ccggcttgtc cttggccttc acgccggtga gcgagcgcat ctgcaggcga
     1201 tccttggcgt aggccagcga gttctggtac gccacttccg ccaggcccag cgactgcatg
     1261 cccacgccca ggcgcgcgcc gttcatcatc acgaacatgg cggccaggcc cttgttgggc
     1321 tcgcccacca tccagccctg cgcgctgtcc aggttgatga cgcacgttgc gttgccgtgg
     1381 atgcccatct tgtgttcgat ggagccgcac ttcacgccgt tgcgcttgcc cggcttgcct
     1441 gcagcgtcgg ggatgaactt gggcaccacg aacagcgaga tgcccttgct gccggccggt
     1501 gcgtccggca aacgcgccag cacgagatgc acgatgttgt cgctcatgtc gtgctcgccc
     1561 gcgctgatga agatcttggt gccgctgatg ctgtagctgc cgtcggcctg cggctcggcg
     1621 cgcgtcttca acaggcccag gtcggtgccg caatgcgctt cggtcaggca catggtgccg
     1681 gtccaggtgc cgtcgatcag ccgcggcagg tacagcttct tgagttcctc ggtgccatgc
     1741 gccagcagcg ccgcgtaggc gccgtgcgac aggcccgggt acatggtcca ggcctggttg
     1801 ccggagttca tcatttccat gaacacgctg ttggtcagtt ccggcaggcc ctggccgccg
     1861 tactcggtag cgctggacag cgccggccag ccggcctcga tgtacttgcg gtaggcctcc
     1921 ttgaagccct tgggcgtggt gaccacgccg tcgccgtgat aggtgcagcc ttcctggtcg
     1981 ccgctctggt tgagcgggaa gatttcgttg gcgacgaact tgccggcctc ctcgacgatc
     2041 gacgacatgg tgtcgatatc gagctcggca tgcttgggca tggcgcggta ctcggtgacc
     2101 acgtccagca cttcctgcag cacgaacttc atgtcgcgca tcggcggtac atacttgccc
     2161 atgaaaaatc tccggaatgg tgaaaaaagg attcgccttc gacaggtctc gggcgggaaa
     2221 atttgagggc gcatagacta gcgcaagcgg gcaacgcccg ccccctgaac gcacgctcac
     2281 atcccatgaa ttcccctgcc cccctcgccg gcccgcgccg ggccgcgctc gtcttcatcc
     2341 tggtcacggt ggccatcgac atcctgtcgt tcggcatcat catcccggta ttgcccaagc
     2401 tggtggtcga tttcgtcggc agcgaagcgg acggcgcacg catgttcggc tggttcagca
     2461 tggcctgggc caccatgcag ttcatcttct cgccgctgct cggtgccatt tcggaccgct
     2521 acggccggcg gccggtgatc ctcatctcct gctttggcct gggcgtggat ttcctggtga
     2581 tggcgctggc gccgaacctc gcgtggctgt tcgccgggcg cctcgtctcc ggcatctgcg
     2641 ccgccagctt ctccaccgcc ggcgcctaca tcgccgacgt gacgccggcg gaaaaacgcg
     2701 ccaccgcctt cggcatgatg ggcgtggcct tcggcatcgg cttcgtggtc gggccgttca
     2761 tcggcggcga actcggtgcc ttgtcaccgc gcgcgccctt ttgggccgca gcctgccttg
     2821 ccctggtcaa cgcctgctac ggcgtgttcg tgctgccgga atcgctgcca accgaaaggc
     2881 gcggcggatt ttcgtgggca cgcgccaacc cgctcggctc gctcaagctg ctgcgctcgc
     2941 accacgagct gttcggtttt gccggcgtga gcttcctggc caacctggcg cacgtggtgc
     3001 tgccgagcac gacggtgctg tacatgggct atcgctatca ctgggatgcg cgcgacgtcg
     3061 gccgcgtgct cgggctggtc ggcatctgct cggccatcgt gcagggcggc ctgatccggc
     3121 ccgcggtgaa gcgctttggc gagcgcaagg ccatgctgtt cgggctcgcc tgcggcacgg
     3181 tgggcttcat gatctacggc ctcgcgccga ccggaacatg gttcctcgtc gccattccca
     3241 tcatggcctt ctggggcctc gccatgccct cgctgcagag cctgatgaca cagcgcgtgt
     3301 cgcccacgga gcagggccag ctgcagggcg ccaactcggc gctgatcggc atcgccggca
     3361 tgatcggccc gggattgttc acgcagacct tcgcgcattt catcgatccc gtcgggctgc
     3421 agctgcccgg cgcgccgtac tacatcgcat ccctgctgtt gttcgtcggg ctgatactgg
     3481 catggcgcat tacgcgctac gcggcggctg cagcggatgt agccactgcc gccgcagcca
     3541 cggagagttg aggtcctcca cggttcagct tcagtacagc ccgccctcgt aaggtgtgcc
     3601 cgttcgcatc gaactgcgat gccaacgacc cttaactgat tgccctaccg ggcggagacc
     3661 tcaacatgaa gaccaagacg attacgcgcc cggcggttct gggcctgctg ttctcggccg
     3721 cgctggtgtc gggcgccgcg tttgcgcaga cggcgccgac aaccaccgtc accacctcga
     3781 ccaccaccac cgtaccggcc gccgaacccg gccatccgcg cgtcaacgaa gtcgaccagc
     3841 gcctggccaa ccagcagaag cgcatcgacg ccggcgtggc cagcggcaag atcaccgcgg
     3901 cgcaggcggc gcgcgacgag aagcgcgatg ccaacatcga agcgcgcgcg caggccgatg
     3961 aggccaagca cggcggccat ctgaccaaga aggagcagga gcgcctgaat ggttcgctga
     4021 acaaggacag caacaagatc cacgagcaga agaccaactg atcgtcggag tcttaatccg
     4081 tctccccgcc gccgcgaggc cgcggggaga tttttttgcg cggtgccaag gtgaccgcgg
     4141 gtgaacaagc tgcgaccgca aatctctaat ccgcagagat gacgaggagc aggccatgaa
     4201 acttttgcat cttgcgctgc tgctgggcgc gcttgccgcc ggtccggcat gggccgacaa
     4261 ggacatgctc ggccgtgaaa tcgaagcgcc gaaaaggcac gcggaaacgc cgccgccggc
     4321 gttgcccggc ctgtcgggcg acgtcaatcc gcaggagccg cagaactcaa gcgaagccga
     4381 cgccgctgcg gcaaagtcac cggcaccggc cgacgcagca gcgttcgagg tccagcccga
     4441 tgccgccggc aagatgcagc agcaatcgcg gcccaagccg ccgccggtgg atcatccgcc
     4501 ggaagatgcc ggtcaccgca agcccgaagg ccagcagcgc tgacgcgtcg cgcataaaaa
     4561 agccggggct cttgcgagcc ccggctttga cagtcggcgc caggaaggcg ccgcgttcgt
     4621 cagggatgat tagaaatcac ccatgccgcc catgccaccg ccacccggcg gcatcatcgg
     4681 cgcttcttcc ttcttcggca gctcggcgat catcgcttcc gtggtgagca gcaggccggc
     4741 gacc
//
LOCUS       DASZZC010000357         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_544906,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000357 DASZZC010000000
VERSION     DASZZC010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544906"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2538
                     /locus_tag="VHE37_07630"
     CDS             <1..2538
                     /locus_tag="VHE37_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35436.1"
                     /translation="VDYDELRQREKQRARVQFAQWTLAACAGIAALAGAWHWQQQRLA
                     EREHQRQLSSWIEKGKQELRSDQYARASAWFGAALAGGADTPALRFLLGWSLRSAHAL
                     TGLAVNHGDTIDRVGYNRSGSSFITYGVGVGKLWDARSGQLLRTLERGPKMLQQMWFA
                     PGDRYFAVSGLLGTDTAQLDNNRAVIWDLSTGRIVCNQPELLWTAAGEPFDAAGERVV
                     TVSNQDSGHGQMKHTVHVWRIRDAQLLLSLPAGAASLPRFSSDGRRLVTGGEDGGPVQ
                     VWDASNGRLLATLHQRPPYADTMAVMTPDGRQVLTAAETGQLRVWDAASGELLDALGN
                     HAGFAPFESNSADGRRWLSVGQDGFRVWDLPAARLLFGGTGVQAYVYDNQLSGDGTLL
                     LTPLAPAGTSLWSVDGKRLLATLDAHAQPATAAALDPAGMHVVTGSGDGSARIWSTAS
                     LAAQPVAALRHGPSIDQAVPAFFWSAQYSADGRRIVTGAQDGIARIWDGSSGALLHEL
                     KGHELGINSVAISADGALAVSGSKDRSVRLWNAAAGTQLHVLSGHDAPIERVLLSPDA
                     QLAASLDRRHQLRVWRTGDGALQATLTLDDPLGINALLFSRDGRYLVASCRNDVLVYA
                     TQDGSLARRLHGHTDRVRGIALSPDGSTLSSAGEDNTLRLWRLDDGSSGGLLDGKDTG
                     VDTGFFLSTAWAPDGHGLIAGTSSGAVILWSPATRRIRTLAAHADMVPQVGYSPDGAL
                     AFSGDRSGNLRIWDADRASLLVEWHAEGGDSVRDIAFSADGTRLLYGEKDSDAAWLLD
                     THREPGSAAQIATFIRCRVPWKVDGDALARSAPDSAACTSVSGGAER"
     gene            2687..3829
                     /locus_tag="VHE37_07635"
     CDS             2687..3829
                     /locus_tag="VHE37_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVT35437.1"
                     /translation="MRRSPLAIAIALSLLLHGYVAWRIVPALALSPAGAMIALLLLCT
                     SAVLLPMPLLIRLSRLPTRAADALAWAGYLCMGMFSTLFVLTLLRDALLALAWIANAA
                     GADLDLASLARSSALGALALAALVSVVGLVNARRVARVVQVELPIENLPPALAGFSIV
                     QISDIHVGPTIKRGYLDAIVRRVNALEADLVAITGDVIDGSVQHLQPHVAPLQQLRSR
                     HGSYIVTGNHEYYHGVHAWLDEWQRLGLRVLHNRGEVIEHGGAKLLIGGVTDFSAHHF
                     DESHRSDAQAAARGDAPVKVLLAHQPRSAPAAEAAGFDLQLSGHTHGGQFWPWNLFVP
                     MQQPFVAGLERLGRLWVYTSRGTGYWGPPIRFGAPSEITRITLVAR"
     gene            complement(3842..>4516)
                     /locus_tag="VHE37_07640"
     CDS             complement(3842..>4516)
                     /locus_tag="VHE37_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVT35438.1"
                     /translation="RVPDAQANLTPIPDGLSDEQVLMCPDIMSTGFGGAESGQIRVGD
                     TVAVFAQGPIGLCAMVGARLRGATRIIAVDRVPERLSMSRQLGADVVIDYTKVDAVQE
                     IMRLTGGAGVDVAIEALGTQATFENCLRVLKPGGTLSSLGVYSGKVSMPVDAFAAGLA
                     DQKIVTTLCPGGKERMRRLMAVIQSGRVDLKPLVTHRFRLDQIEQAYDLFAHQRDGVL
                     KVAITP"
BASE COUNT          688 a         1599 c         1540 g          689 t
ORIGIN      
        1 gtggattacg acgagttgcg ccagcgcgag aaacagcgcg cgcgtgtgca attcgcgcaa
       61 tggacgctgg cggcgtgtgc cggcatcgcg gcgctggccg gtgcctggca ctggcagcag
      121 cagcgcctgg ccgaacgcga acaccagcgc cagctgtcca gctggatcga gaagggcaag
      181 caggaactgc gcagcgacca gtacgcgcgc gcgtcggcat ggttcggtgc cgcgctcgcc
      241 ggcggcgccg acacgccggc gctgcgcttc ctgctcggct ggtcgctgcg ctccgcgcat
      301 gcgctcaccg gccttgcggt gaaccacggc gacaccatcg accgcgtggg gtacaaccgc
      361 agcggcagca gcttcatcac ctacggtgtg ggcgtgggca agctgtggga cgcgcgcagt
      421 ggccagctgc tgcgcaccct ggagcgcggg ccgaagatgc tgcagcagat gtggtttgct
      481 cccggcgacc gttatttcgc cgtatccgga cttctgggca ccgacactgc gcaactggac
      541 aacaaccgcg ccgtcatctg ggacctgtcc accggccgaa tcgtgtgcaa tcagccggaa
      601 ttgctgtgga ccgcggccgg cgaaccgttc gacgccgccg gcgagcgcgt ggtcaccgtc
      661 agcaaccagg actccggcca cggacagatg aagcacaccg tgcacgtgtg gcgcatccgc
      721 gatgcgcagc tgctgctctc gctgcccgcc ggcgcggcca gcctgccgcg cttcagcagc
      781 gacggtcggc ggctggtgac cggcggcgag gacggcggtc cggtgcaggt ctgggacgcc
      841 agcaacgggc gcctgctcgc cacgctgcac cagcgcccgc cgtatgccga caccatggcc
      901 gtaatgacac ccgacggacg ccaggtgctc acggccgccg aaaccgggca gctgcgcgtg
      961 tgggacgcgg ccagcggcga gttgctcgat gcgctgggca atcacgccgg cttcgcgccc
     1021 ttcgaatcga actcggccga cggccgccgc tggctgtcgg tgggccagga cggtttccgc
     1081 gtgtgggatc tgcccgccgc gcggctgctg ttcggcggca ccggcgtgca ggcctacgtt
     1141 tacgacaacc agctcagcgg cgacggcacg ctgctgctga cgccgctggc gccagccggc
     1201 accagcctgt ggagcgtgga tggcaaacgc ctgctggcga cgctcgacgc gcacgcgcag
     1261 ccagccacgg ccgccgcgct cgatcccgcg ggcatgcatg tcgtcaccgg cagcggcgac
     1321 ggcagcgcgc gcatctggtc gaccgcgtcg ctggccgcgc aaccggtggc cgcgctgcgt
     1381 cacggcccct ccatcgacca ggcggtgccg gcatttttct ggagcgcgca gtacagcgcc
     1441 gatggccgcc gcattgtcac cggcgcgcag gacggcatcg cacgcatctg ggacggcagc
     1501 agcggcgcgc tgctgcatga actgaagggc catgaactgg gcatcaacag cgttgcgatc
     1561 agcgccgacg gcgcgttggc cgtcagcggc tccaaggacc ggagcgtgcg cttgtggaat
     1621 gctgccgccg gcacccaact gcacgtgctc tccgggcacg atgcgccgat cgagcgcgtg
     1681 ctgctgagcc cggacgcgca gctcgccgca agcctcgacc gccggcacca gctgcgcgtg
     1741 tggcgcaccg gcgatggcgc gctgcaggcg acgctgaccc tcgacgaccc cctcggcatc
     1801 aacgcgctgc tgttcagccg cgacggccgc tacctggtcg ccagctgccg caacgacgtg
     1861 ctggtctatg ccacgcagga cggctcgctc gcgcggcgcc tgcatggaca caccgaccgc
     1921 gtacgcggca tcgcgctgtc gccggacggc agcacattgt ccagtgccgg cgaggacaac
     1981 acgctgcgcc tgtggcggct ggacgatggc agcagcggcg gattgctcga cggcaaagac
     2041 accggcgtcg acaccggctt cttcctctcg acggcctggg cgccggatgg acatgggctg
     2101 attgccggca ccagcagcgg cgcggtgatc ctgtggtcgc cggcaacacg ccgcatccgc
     2161 acactggccg cgcatgcgga catggtcccg caggtgggtt acagcccgga tggagcgctg
     2221 gccttttccg gcgaccgcag cggcaacctg cgcatctggg acgcggaccg cgccagcctg
     2281 ctggtcgagt ggcatgcgga aggcggcgac tcggtgcgcg atattgcctt cagcgccgat
     2341 ggcacgcgcc tgctctacgg tgagaaggac agcgatgccg cgtggctgct cgacacccac
     2401 cgcgaacccg gcagcgccgc gcagatcgcc accttcatcc gctgccgcgt gccgtggaaa
     2461 gtcgatggcg atgcgctggc gcgcagcgcg ccggacagcg cggcctgcac cagcgtgagc
     2521 ggcggcgcag aacgctgaaa accgcctgac cgcactgccg ttgcgcgtgt tatttattat
     2581 taagtatatt ttaaaatatt aaatacttaa taacatcaca acagccggtg cggtgaggca
     2641 aaaaaacgct cttgaatcct tcgcagccac atccatcttc cagccgatgc gccgctcccc
     2701 gcttgccatt gccattgcgc taagcctgct gctgcacggc tacgtggcgt ggcgcatcgt
     2761 tcccgcactc gcactttcgc cagccggcgc gatgatcgcg ctgctgctgc tctgcacctc
     2821 cgccgtgctg ctgccgatgc cgctgctgat ccgcctgagc cggctgccga cgcgcgcggc
     2881 ggacgccctg gcctgggccg gttatctctg catgggcatg ttctccacgc tgttcgtgct
     2941 cacgctgctg cgcgacgcgc tgctcgcgct ggcgtggatc gccaatgcag caggtgcaga
     3001 tctcgatctc gcttcactcg cgcgaagcag cgcgctgggc gcgctggcgc tggccgcgct
     3061 ggtgtcggtc gtaggcctgg tcaatgcgcg gcgcgtggcg cgcgtggtgc aggtggaact
     3121 gccgatcgag aacctgccgc cggcactggc cggcttcagc atcgtgcaga tcagcgacat
     3181 ccatgtcggg ccgaccatca agcgcggcta cctcgacgcc atcgtgcggc gggtgaatgc
     3241 gctcgaggcc gacctggtgg cgatcaccgg cgacgtcatc gacggcagcg tgcagcatct
     3301 gcagccgcac gtggcgccgc tgcaacagct gcgctcgcgc catggcagct acatcgtcac
     3361 cggcaaccac gagtactacc acggcgtgca tgcctggctc gacgaatggc agcgcctggg
     3421 cctgcgcgtg ctgcacaacc gcggcgaggt gatcgagcac ggcggcgcga aactgctgat
     3481 cggcggcgtc accgatttca gcgcgcacca cttcgacgag tcgcatcgca gcgacgcgca
     3541 ggccgctgca cgcggcgatg ccccggtgaa agtcctgctc gcgcaccagc cgcgcagcgc
     3601 gcccgccgcg gaagccgccg gcttcgacct gcagctctcc ggccacacgc acggcggcca
     3661 gttctggccg tggaatctgt tcgtgccgat gcagcagccg ttcgtggccg gactggaacg
     3721 gctcggcagg ctgtgggtct acaccagccg cggcaccggc tactgggggc cgcccatccg
     3781 cttcggcgcg ccatcggaga tcacgcgcat cacgctggtc gcgcgctgac ccgcgcagcg
     3841 gtcagggcgt gatcgccacc ttgagcacgc cgtcgcgctg gtgcgcaaac aggtcatagg
     3901 cctgctcgat ctggtcgagc ctgaagcggt gcgtgaccag cggcttgagg tccacgcgtc
     3961 ctgactggat caccgccatc agccggcgca tgcgctcctt gccgccggga cacagcgtgg
     4021 tgacgatctt ctgatcggcg agcccggccg caaacgcgtc gaccggcatg ctgaccttgc
     4081 cggaatagac gcccaggctc gacagcgtgc cgcccggctt gagcacgcgc aggcagttct
     4141 cgaacgttgc ctgcgtgccc agtgcctcga tggcgacatc gacgccggcg ccaccggtga
     4201 gccgcatgat ctcctgcacc gcgtcgacct tggtgtagtc gatcaccacg tcggcgccga
     4261 gctggcgcga catgctcagc cgttcgggca ctcggtccac cgcgatgatg cgcgtggcgc
     4321 ccctgagccg cgcgccgacc atggcgcaca ggccgatcgg cccctgcgcg aacaccgcca
     4381 ccgtgtcgcc gacgcggatc tgcccgctct cggcgccgcc gaagccggtg ctcatgatgt
     4441 cggggcacat cagtacctgc tcgtcgctga gcccgtcggg gatcggcgtc aggttggcct
     4501 gcgcgtcggg cacgcg
//
LOCUS       DASZZC010000358         5507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_544995,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000358 DASZZC010000000
VERSION     DASZZC010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544995"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..439)
                     /locus_tag="VHE37_07645"
     CDS             complement(86..439)
                     /locus_tag="VHE37_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35439.1"
                     /translation="MVSTSGTCSTFSNWCAMRLMSATTSLLALSPISRPLVSSASQNA
                     VEPITSAISTEPMPSHSRLPVTCSSSSAPSASSRPSTAAESSSSTANFDGSLPCSTAS
                     TCVQWPRVREKRRAA"
     gene            complement(600..1019)
                     /locus_tag="VHE37_07650"
     CDS             complement(600..1019)
                     /locus_tag="VHE37_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVT35440.1"
                     /translation="MRSHRKELWFNTPTRLAFLNITPQVEQALAESGVREGLCLVNAM
                     HISASVFINDDERGLHQDFTRLLEQWVPHEPVAQYRHNDTGEDNADAHIKRQLMGREV
                     VVAITGGKLDFGPWEQIFYGEFDGRRKKRVLVKIIGE"
     gene            complement(1077..1298)
                     /locus_tag="VHE37_07655"
     CDS             complement(1077..1298)
                     /locus_tag="VHE37_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HVT35441.1"
                     /translation="MKVAGIILLVVGIISLAYQGFSYDRTERVADVGPVEIDKTTTRH
                     VPLPPVLGGLAMVGGIVLLLAGRRGRSNF"
     gene            1438..1755
                     /locus_tag="VHE37_07660"
     CDS             1438..1755
                     /locus_tag="VHE37_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35442.1"
                     /translation="MPPSLQGSFDHRMENLGPARRAQHNLHLLLADWKRGNWLMRYFL
                     ASQVGATALSMICSAGSIAMLATGNMAANLWLLCAAGISFIHAVSTFGVLKRRYQVRY
                     AVW"
     gene            complement(1762..2253)
                     /locus_tag="VHE37_07665"
     CDS             complement(1762..2253)
                     /locus_tag="VHE37_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT35443.1"
                     /translation="MRVTQLLFACALLCGCTTMQTRVAPDRATLEQQVRDTEAAFARS
                     MAQRDFEAFKGFLSDEAVFFSGSEPLRGRAAIERRWQHLFEGKQAPFSWQPDTVQVLD
                     SGTLALSSGPVYGPDGKQTATFNSIWRRDADGVWRVIFDKGCSACPCADLPHPTPSHK
                     PMS"
     gene            complement(2311..3060)
                     /locus_tag="VHE37_07670"
     CDS             complement(2311..3060)
                     /locus_tag="VHE37_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT35444.1"
                     /translation="MSQPVTIIGTYLSPYVRKVLAVLHLKNIDYRIDPIVGFMGNDEF
                     SRISPLRRIPVLVDDAVTLCDSSVICEYLDERYPQVRVYPRDIGERARARWLEEYADT
                     RMGDVFIWQLFNQRVIAPHLFGKTIDETILRRALNQEIPQVLDHLESFAPRIGLLFGE
                     VTVADLALAVFFRNAAFARWSIDAQRWPRTAAYVERVLELDCLKRLRPYEDLIARTRP
                     PQQRAALAGLGAPLTPTSYAASRPRPGIMRI"
     gene            complement(3119..3832)
                     /locus_tag="VHE37_07675"
     CDS             complement(3119..3832)
                     /locus_tag="VHE37_07675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVT35445.1"
                     /translation="MSAALSPETARHRRRMAFAGASLLLALLLLAAAWRWTPLHAYAD
                     PRLLADHLQDLQSQWWTPLLVMLVYVVANAMLFPNTILNVATILGLGTAMGVPCALLG
                     SLAASVVFYAFGLRYGKEQLRKLESIKLERLARVLRDGGIVSVAMVRLVPVAPHTVVN
                     IAAGAMHVRFWPFVIGTTLGLLPGNLLVGAFGAQLRAVLRHPTPFELVLLSSIALLAL
                     ACVWWTSRRLSTFGHSLPH"
     gene            complement(3837..4370)
                     /locus_tag="VHE37_07680"
     CDS             complement(3837..4370)
                     /locus_tag="VHE37_07680"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HVT35446.1"
                     /translation="MVSTSLDREARRATLLRFRQHDDKARPQNATARAPTLVVAADAS
                     RARLLIALPGEPGLREVDDLLNPEARLHEGDLVADGPGRRNNSSTSGGYSALGGGSMK
                     RHRIEEFASLVCKRVERCLEDNLARRVYLVAQPEFLGLLRKQARPRLVRSFAGEVAKA
                     LTGAAPAEIRAVLPARL"
     gene            4462..4833
                     /locus_tag="VHE37_07685"
     CDS             4462..4833
                     /locus_tag="VHE37_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT35447.1"
                     /translation="MIGYVTLGTNDLPRAAKFYDALLAEIGAKRWMEFDTFIAWAVSP
                     DKPSLGVIKPYDKKPATVGNGVMVALVVDSKDKVDRIYKKAISLGAQDEGPAGPRGDG
                     FYAGYFRDLDGNKLNCFYMGK"
     gene            complement(4891..5208)
                     /locus_tag="VHE37_07690"
     CDS             complement(4891..5208)
                     /locus_tag="VHE37_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HVT35448.1"
                     /translation="MAAKKKAAAKAKPKKAAPKKAASKNRGGIAQVVSLSPDLQAVVG
                     ASSLTRAELTKKVWDYIKKNGLQDAKIRRNINADEKLGKIFGGKKQVSMFEMTKLVNK
                     HVK"
BASE COUNT          900 a         1801 c         1869 g          937 t
ORIGIN      
        1 tgctgcatcc gcagccgctg gagaacgccg cgctgaacta cgtggtgctg gccatctcgc
       61 tgctgttcga gggcgcgacc tggttctacg cggcgcggcg cttctcgcgc acgcgcggcc
      121 actggacaca cgtggaagcg gtgctgcacg gcaaggaccc gtcgaagttc gccgtgctgc
      181 tggaggattc cgccgccgtg ctcggcctgc tgctggcgct gggcgcgctg ctgctcgagc
      241 aggtcaccgg caacctgctg tgggacggca tcggctcggt gctgatcgca ctggtgatgg
      301 gctccaccgc gttctggctg gcgctggaga ccaagggcct gctgatcggc gagagcgcca
      361 acagcgaggt ggtggccgac atcaagcgca tcgcgcacca gttcgaaaag gtgctgcagg
      421 tgccggaggt gctgaccatg cacgtggggc cggattacat cctggtcaac ctgtccatcg
      481 tgattcccgc cggcgtcacc gccgccaccg cggaaagacc cgcacgcagt tgcagcaatg
      541 catccaccag aagcatccgc gggtcaagcg catcttcgtc gagacacgca ccgcgtgaat
      601 cattcgccga tgatcttcac cagcacgcgc ttcttgcgcc ggccgtcgaa ttctccataa
      661 aagatctgtt cccacgggcc gaagtcgagc ttgccgccgg tgatggcgac caccacttcg
      721 cggcccatca gctggcgctt gatgtgcgca tcggcgttgt cctcgccggt gtcgttatgg
      781 cggtactgtg cgacgggttc gtgcgggacc cactgctcga gcaggcgggt gaagtcctgg
      841 tgcaggccgc gctcgtcgtc gttgatgaag accgaggcgc tgatgtgcat ggcgttgacc
      901 aggcacaagc cttcgcgcac gccgctctcg gccagcgcct gctccacctg cggcgtgatg
      961 ttgaggaagg ccaggcgggt gggagtgttg aaccagagtt ccttgcggtg cgatctcatg
     1021 aaaaatcccg gctgctcctg gcggagcttg ccgggatgtg cgggcagcga caaggctcag
     1081 aagttggagc gtccacgcct gccggccagc agcaggacaa tgccgccgac catggccagc
     1141 ccgcccagca ccggcggcag cggcacgtgg cgcgtggtgg tcttgtcgat ctccaccggg
     1201 ccgacgtcgg cgacgcgttc ggtgcggtcg tagctgaaac cctggtaggc cagcgagatg
     1261 atgcccacta ccaacagtat gatgcctgcg actttcatgg ctgcctcctc acggtttccc
     1321 tacatcaggc ccgcccgcgg ccggcaaagt tccttcggtt ttgctccggc cggcttgtgc
     1381 agggggccta gacaaaaggg cttaggggca tgaattgcgt ccggggcggc gctatgcttg
     1441 ccgccgtcgc tccagggaag tttcgaccac cgcatggaaa atctcggccc cgcgcgccgc
     1501 gcgcaacaca atctgcatct gctgctggct gactggaaac gcggcaactg gctgatgcgc
     1561 tacttcctcg ccagccaggt cggcgccacc gcgctgtcga tgatctgctc cgccggcagc
     1621 atcgccatgc tggccaccgg caacatggca gccaacctgt ggctgctgtg cgcggcgggc
     1681 atcagcttca tccacgcggt cagcaccttc ggcgtgctca agcgccgcta ccaggtgcgc
     1741 tacgccgtct ggtagcgcgg gctacgacat cggcttgtgc gacggcgtcg ggtgcggcag
     1801 gtccgcgcac gggcaggccg agcaaccctt gtcgaagatc acgcgccaca cgccatcggc
     1861 atcgcgccgc cagatcgagt tgaaggtcgc cgtctgcttg ccgtcgggtc cgtagaccgg
     1921 cccgctcgac agcgccagcg tgccgctgtc cagcacctgc accgtatcgg gctgccacga
     1981 gaacggcgcc tgcttgccct cgaacaggtg ctgccagcgc cgctcgatcg ccgcacgtcc
     2041 gcgcagcggt tcgctgccgg aaaagaacac cgcctcgtcc gacaggaagc ccttgaacgc
     2101 ttcgaagtcg cgctgcgcca tgctgcgcgc gaaggccgcc tcggtgtcgc gcacctgctg
     2161 ctcgagcgtg gcgcggtccg gcgccaccct ggtctgcatg gtcgtacagc cgcacagcag
     2221 cgcgcaggcg aacagcagtt gggtgacacg catgtcggct cccggtctgg atcgttatag
     2281 agacaacggc aacgggccca aggttgggct tcagatgcgc atgatgccgg gccgcggccg
     2341 gctggcggcg tagctggtcg gcgtcagcgg cgcgcccagc ccggccagcg ccgcgcgctg
     2401 ctgcggcgga cgcgtgcgcg cgatcaggtc ctcgtacggg cgcaggcgct tgaggcaatc
     2461 gagttcgagc acgcgctcca catacgccgc ggtgcgtggc cagcgctgcg cgtcgatgct
     2521 ccagcgggca aaggccgcat tgcggaagaa cacggcgagc gcaaggtccg ccaccgtcac
     2581 ctcgccgaac agcaggccga tgcgcggcgc gaaactttca agatggtcca gcacctgcgg
     2641 gatttcctgg ttgagcgcgc ggcgcaggat cgtctcgtcg atggttttgc caaacaggtg
     2701 cggcgcgatc acgcgctggt tgaacaactg ccagatgaac acgtcgccca tgcgcgtgtc
     2761 ggcatattcc tccagccagc gcgcgcgggc gcgctcgccg atgtcgcgcg gatacacccg
     2821 cacctgcggg tagcgctcat cgaggtattc gcagatcacg ctggagtcgc acagcgtcac
     2881 cgcgtcgtcg accagcaccg ggatgcggcg cagcgggctg atgcgcgaga actcgtcgtt
     2941 gcccatgaag ccgacgatgg ggtcgatgcg gtagtcgatg ttcttcagat gcagcacggc
     3001 cagcaccttg cgcacgtagg gcgacaggta ggtgccgatg atggtgacgg gctggctcat
     3061 gcgcggctct cctgtgagcg cggcacgata acgcgcgggc cgtggatgaa tccagcgctc
     3121 aatggggcag cgagtggccg aaggtgctga gccggcggct ggtccaccac acgcaggcca
     3181 gcgcgagcag cgcgatcgac gacagcagca ccagttcgaa tggagtcggg tgccgcagca
     3241 ccgcgcgcag ctgcgcgccg aaagcgccga ccagcaggtt gcccggcagc agtcccagcg
     3301 tggtgccgat gacgaacggc cagaagcgca cgtgcatggc gccggcggcg atattgacca
     3361 cggtgtgcgg cgccaccggc accagccgca ccatggccac gctgacgatg ccgccgtcgc
     3421 gcagcacgcg cgccagccgt tcgagcttga tgctttccag cttgcgcaac tgctccttgc
     3481 cgtagcgcag gccgaaggca tagaacacca ccgaggccgc caggctgccg agcaatgcgc
     3541 agggcacgcc catcgccgtg cccaggccga ggatggtggc gacgttgagg atggtgttgg
     3601 ggaacagcat ggcgttggcc accacgtaga ccagcatcac cagcagcgga gtccaccact
     3661 ggctctgcag gtcctgcagg tggtcggcga gcagcctggg atcggcgtag gcgtgcagcg
     3721 gcgtccagcg ccatgccgcc gccagcagca gcagcgcgag cagcaggctg gcacctgcaa
     3781 acgccatgcg gcggcggtgc cgcgcggtct ccggcgacag tgccgcggac atcggatcag
     3841 aggcgtgcgg gcaggacggc gcggatctcg gccggtgcgg cgccggtcag cgccttggcc
     3901 acttcgccgg cgaagctgcg caccagtcgc ggccgcgcct gcttgcgcag caggccgagg
     3961 aattccggct gcgctaccag gtacacgcgg cgcgccaggt tgtcctccag gcagcgctca
     4021 acacgcttgc acaccagcga ggcgaattcc tcgatgcgat gccgcttcat cgagccgccg
     4081 cccagcgcgg agtagccgcc ggaggtggag ctgttgttgc gccggccggg cccgtcggcc
     4141 accaggtcgc cttcgtgcag gcgcgcttcg gggttgagca ggtcgtccac ttcgcgcaac
     4201 cccggctcgc cgggcagggc gatgagcagc cgcgcgcgcg aggcatccgc cgccaccacc
     4261 agcgtgggcg cacgtgccgt ggcgttctgc gggcgggctt tgtcgtcgtg ctggcggaag
     4321 cgcagcaggg tggcgcggcg ggcttcccga tcgagactgg tgctgaccat cgtgtcgtcc
     4381 tcctgtcgtt tttagagcgc agattttttt ccctggttcg gccgcgtaac atgcccgggc
     4441 catccaaccg gagaacaaga catgattggc tacgtaaccc tgggaaccaa cgatctgccg
     4501 cgcgcagcga agttttacga cgcgctgctg gcggaaatcg gcgccaagcg ctggatggaa
     4561 ttcgacacct tcatcgcctg ggcggtgagc ccggacaagc cgagcctggg cgtgatcaag
     4621 ccctacgaca agaagcccgc gacggtgggc aacggcgtga tggtggcgct ggtggtcgac
     4681 agcaaggaca aggtcgaccg catctacaag aaggccatct cgctgggcgc gcaggatgaa
     4741 ggcccggccg gtccgcgcgg cgacggcttc tatgcaggct acttccgcga cctggacggc
     4801 aacaagctca actgcttcta catgggtaaa taaggcgcac aaaaaaagcg ggcggcccgt
     4861 gggccgcccg ctgtgcagaa agcgtgctgc ttacttgacg tgcttgttga ccagcttggt
     4921 catctcaaac atcgacacct gcttcttgcc gccgaagatc ttgccgagct tctcgtcggc
     4981 gttgatgttg cggcggatct tggcgtcctg caggccgttc ttcttgatgt agtcccagac
     5041 cttcttggtc agctcggcgc gggtcagcga gctcgcaccg accacggcct gcagatccgg
     5101 gctcagcgaa acaacctgcg cgataccgcc gcggttcttc gacgcggcct tcttcggcgc
     5161 ggccttcttg ggtttggctt ttgcagcggc tttcttcttt gcggccattc gactctcctg
     5221 tgtgtagtgt ggctggcttg atgatgtggt ccgggtctgg cccgggcgcg catgttatac
     5281 cccgagtctc gaccatcgtc gagacgcgcg aattccgagg ccagcgcggg aaattgtcaa
     5341 gcgtttttca tcgttgcggc gccttgccgc cggtggtcaa atcgccagca tttttcgggg
     5401 ggaaatgcaa tgagcgagtc gatcgtgatc accggtgcgt cgtcgggcct gggcgaagcg
     5461 atggcctacg aattcgccgc gcgcggttac cggctcggtc tcaccgc
//
LOCUS       DASZZC010000359         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_222176,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000359 DASZZC010000000
VERSION     DASZZC010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222176"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VHE37_07695"
     CDS             <1..550
                     /locus_tag="VHE37_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35449.1"
                     /translation="ELREAALAFSCANGFYAIAAARAAPQRVRRLLLCQTPGLSAMPP
                     WTQLNVPWPVRTPLLGQALVRATRRTVARGWYGGAEPDREQRAKLRATADHALAHGGC
                     FCLAGVVQGLSRARPEELAGVKCPAQLIWGDADKSHRHTRAESLQELLPQASVEHWPE
                     CGHFPELSQPRRYAERVLAGVC"
     gene            714..1574
                     /locus_tag="VHE37_07700"
     CDS             714..1574
                     /locus_tag="VHE37_07700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HVT35450.1"
                     /translation="MPALRTHEIPKRDLQFDFSKVDLLRWHGLGTIASHWFNVQSLMF
                     PQGERYFIRSVRRYRDRIRDPQLQEQIAGFIAQEAMHGREHDAYNAALARAGYPVAWM
                     ERWVSVLIALAEKAQPPSARLAETAGLEHLTAIGAEVLLSNDDAMKDCDPTMAQLWRW
                     HAVEETEHKAVAFDVHREIMGRGPFAWLQRSWTLLVGSIGMTFTTWLFLLVAAWRTRT
                     LFDLRGWGRLLWALWIYPGATRRQVIPYFKYFSPWFHPWNSDNYEQVLRWRQGQISPA
                     PGAEPLLQRG"
     gene            complement(1686..2699)
                     /locus_tag="VHE37_07705"
     CDS             complement(1686..2699)
                     /locus_tag="VHE37_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HVT35451.1"
                     /translation="MKQIWITGTGGPEKLQLREAPDPAPAAGELRIRVRASGINFADI
                     LARQGLYPDAPKIPCVVGYEVSGTVDAAGAGAESWIGKDAYALTRFGGYSDVVCVPAT
                     QCFERPAALTHEQCAAIPVNYLTAWQLLVTMGSLSQDETVLIHNAGGGVGLAAIDIAR
                     HIGARIIGTASSGKHAFLKQRGLHEAIDYRTQDWEAELKKLTDGGGVELITDPMGGSH
                     WKKSYRALRATGRVGMFGISAATESSLLGPLRLLPVALGMPLFHPIPLMNQNRSAFGV
                     NLGHMWHETAKIRSWMSALLEGVRDGWVRPHVDKSFSFAQAGEAQAYMEARKNIGKVV
                     LVP"
BASE COUNT          426 a          917 c          943 g          473 t
ORIGIN      
        1 ggagctgcgc gaggctgcgc tcgccttcag ctgcgccaat ggcttctatg ccatcgccgc
       61 cgcgcgcgcg gcgccgcagc gcgtgcgacg cctgctgctg tgccagacgc cgggcctgtc
      121 ggcgatgccg ccgtggacgc agctgaatgt gccgtggccg gtccgcacgc cgctgctcgg
      181 ccaggcgctg gtgcgcgcca cgcggcgtac cgtggcgcgc ggctggtacg gcggcgccga
      241 gccggaccgc gagcagcgcg cgaagctgcg cgccaccgcc gatcacgcgc tggcacacgg
      301 cggctgcttc tgcctggccg gcgtggtgca gggcctgtcg cgggcgcgcc cggaagagct
      361 cgccggcgtg aaatgtcctg cgcagctgat ctggggcgac gccgacaaga gccatcgcca
      421 cacgcgcgcc gagtcgctgc aggaactgct gccgcaggcg agcgtcgaac actggccgga
      481 gtgcggacat tttcccgagc tgtcgcagcc gcgccgctac gccgagcgcg tgctggcggg
      541 tgtttgctga gtcccccgca agccgttttt cgttgttatt aattattatg caaatttaaa
      601 aaatatatat atttaataac atcgagaagc agggtgcggt caggcacctt ttgcgcgttg
      661 gagtgccgcg cgcatcggcg ctagcatctg gcaccacgct ccaggagcag gccatgcccg
      721 cattgcgcac gcacgaaatt cccaaacgcg acctgcagtt cgatttctcg aaagtcgacc
      781 tgctgcgctg gcacggcctg ggcaccatcg ccagccactg gttcaacgtg cagtcgctga
      841 tgttcccgca gggcgagcgc tacttcatcc gcagcgtgcg ccgctaccgc gaccgcatcc
      901 gcgatccgca gctgcaggaa cagatcgccg gcttcatcgc gcaggaggcc atgcacggcc
      961 gcgagcacga cgcctacaac gccgcgctgg cgcgcgccgg ctacccggtg gcgtggatgg
     1021 agcgctgggt gtcggtgctg attgcgctgg cggaaaaggc gcagccgccg tcggcgcggc
     1081 tggcggaaac cgcggggctg gaacacctca ccgccatcgg cgccgaggtg ctgctgtcca
     1141 acgacgacgc gatgaaggac tgcgatccca ccatggcgca gctgtggcgc tggcacgcgg
     1201 tggaggaaac cgagcacaag gcggtggcgt tcgacgtgca ccgcgagatc atgggccgcg
     1261 gccccttcgc ctggctgcag cgcagctgga cgctgctggt tggcagcatt ggcatgacct
     1321 tcaccacctg gctgttccta ctggtagcgg cctggcgcac gcgcaccttg ttcgacctgc
     1381 gcggctgggg ccggctgctg tgggcgttat ggatttatcc gggcgcgacg cggcgccagg
     1441 tgattccgta cttcaaatac ttcagcccgt ggttccatcc gtggaacagc gacaactacg
     1501 agcaggtgct gcgctggcgg caggggcaaa tctccccggc gccaggcgcc gagcctcttc
     1561 tgcaaagagg gtaagcaaat acaaaacgtg ttttctgttc ctgagccctc tttgaaaaag
     1621 agagcggcga gcgagcggga gatttcagcg attccgcctg atggcgcacg ccgacgcagc
     1681 gcgcctcacg gcaccagcac caccttgccg atgtttttcc gcgcctccat gtaggcctgc
     1741 gcctcgcccg cctgcgcaaa ggaaaagctc ttgtcgacgt gcggccgcac ccagccgtcg
     1801 cgcacgcctt cgagcagcgc gctcatccag ctgcggatct tcgccgtctc gtgccacatg
     1861 tggcccaggt tgacgccgaa ggcggagcgg ttctggttca tcagcggaat cgggtggaac
     1921 agcggcatgc ccagcgccac cggcagcagg cgcagcgggc cgagcaggct ggattcggtg
     1981 gcggcggaaa tgccgaacat gcccacgcgc ccggtggcgc gcagcgcgcg gtagctcttc
     2041 ttccagtggc tgccgcccat ggggtcggtg atcagttcca cgccgccgcc atcggtgagt
     2101 ttcttcagtt ccgcttccca gtcctgcgtg cggtagtcga tcgcttcatg cagcccgcgc
     2161 tgtttcagga aggcatgctt gccgctgctg gcggtgccga tgatgcgcgc gccgatgtgc
     2221 cgcgcaatgt cgatggccgc caggcccacg ccgccgccgg cgttgtggat cagcacggtc
     2281 tcgtcctgcg acagcgagcc catcgtcacc agcagctgcc aggcggtgag gtaattgacc
     2341 gggatggctg cgcattgttc gtgcgtgagc gcggccgggc gctcgaagca ttgcgtcgcc
     2401 ggcacgcaga ccacgtcgct gtatccgccg aagcgggtga gggcgtaggc gtccttgccg
     2461 atccagcttt cggcgccggc gccggccgcg tcgaccgtgc ccgacacctc gtagccgacc
     2521 acgcagggaa ttttcggcgc gtcggggtag aggccctggc gcgcgaggat gtcggcaaag
     2581 ttgatgccgc tggcgcgcac gcggatgcgc agctcgccgg ccgccggtgc gggatcgggc
     2641 gcttcgcgca actgcagttt ttccggtccg ccggtgccgg tgatccagat ctgcttcatg
     2701 cgcgcgctcc gcctctggtg agggcgctac gctagccgag tgcggcagtg aaatctatc
//
LOCUS       DASZZC010000360         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_478393,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000360 DASZZC010000000
VERSION     DASZZC010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478393"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1060
                     /locus_tag="VHE37_07710"
     CDS             176..1060
                     /locus_tag="VHE37_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HVT35452.1"
                     /translation="MTDQLTDFVFPQLGARGAVVEISAGVEAMLGSREYAPAVRRLLG
                     QALAATPLLASHSKFDGRINLQFQNKRAGTGAIQLLVTQIDREFNLRGMAKAPADADG
                     TFQQLMGGGVLALMLEPRSGAQNYQALVEILGDSLAESLEIYFAHSEQLPTLLRLAAH
                     GNRVAGMLLQRLPQGEIGSSDDSWEHLKILFGTLGEDELGATAPETLLRRLFHAEELR
                     LAPPRPIVLSCRCSHASISAMLLALGEAELQPVLEEQGKIDVTCEFCGRAYSYNALQM
                     RDLFAAELRQPDASTKLN"
     gene            1142..1600
                     /locus_tag="VHE37_07715"
     CDS             1142..1600
                     /locus_tag="VHE37_07715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HVT35453.1"
                     /translation="MIIPKYSIRSSTIAGAGKGLFLDEPVSRGAVVVAPDRIDRMMHL
                     RELEKFPKDSIENISSARWFEDWFSISPEWPDECYVNHSDAPNGLWHLGFIFAARDLA
                     AGEELTADYRYILGSHEPAGFADSATGRVVVGLEWKENVRRSAEALLRLL"
     gene            1805..>2538
                     /locus_tag="VHE37_07720"
     CDS             1805..>2538
                     /locus_tag="VHE37_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HVT35454.1"
                     /translation="MQPAPAFGITVVIAVSAKTGAWLWQRATRNAGMVDAVWAWTLAM
                     IALFYAAVGSAPPATRTQLALMGALWGMRLGVYLFIRNHGKPEDWRYASFRAQWGAGA
                     DRKMFWFFQFQNVFTLLLSASAFLTVAYRPGNAPPAACWAAVALWLVSVLGETLADRQ
                     MKHFRANPANKGRVCRDGLWNWSRHPNYFFECVHWTAYISLAWGAPMMWASFAAPLVM
                     AWLLLRLSGVPMLEKEMIARKPGYAE"
BASE COUNT          418 a          829 c          852 g          439 t
ORIGIN      
        1 cgtcgccggc atcaagcggc tgttccagca gagctggacg cgcggcatgc gcggcgcttt
       61 cggcctggag agccggctgc agctgcgcat gttccgcacc cgcaaccagc gcgaggcggc
      121 gcggtccaac ttcgagaagc gtgcgccgaa gtacggcccg cgccagatcg actgaatgac
      181 tgaccagctc accgacttcg tctttcccca gctgggcgcg cgcggcgcgg tggtcgagat
      241 cagcgccggt gtcgaggcca tgctcggctc gcgcgagtac gcgccggcgg tgcgccgcct
      301 gctgggacag gcgctggcgg caacgccgct gctggcctcg cactcgaaat tcgacggccg
      361 catcaacctg cagttccaga acaagcgcgc cggcaccggc gccatccagc tgctggtcac
      421 gcagatcgac cgtgagttca acctgcgcgg catggccaag gcgcccgccg acgcggacgg
      481 aacgttccag cagctgatgg gcggcggcgt gctggcgctg atgctggagc cgcgcagcgg
      541 cgcgcagaat taccaggcgc tggtggagat cctgggcgat tcgctggccg agtccctgga
      601 aatttacttc gcccattccg agcagctgcc cacgctgctg cgcctggccg cgcacggcaa
      661 ccgcgtggcc ggcatgctgc tgcagcgcct gccgcagggc gagatcggca gcagcgacga
      721 cagctgggaa cacctgaaga tcctgttcgg cacgctgggc gaggacgagc tcggtgccac
      781 cgcgcccgag accttgctgc ggcgcctgtt ccatgccgag gaactgcgcc tggcgccgcc
      841 gcgcccgatc gtgctgtcgt gccgctgcag ccatgccagc atttccgcca tgctgctggc
      901 gctgggcgag gcggaactgc agccggtgct cgaggagcag ggcaagatcg acgtcacctg
      961 cgaattctgc ggccgcgcgt attcctacaa cgcactgcag atgcgcgacc tgtttgccgc
     1021 agagctgcgt caacccgatg cttccaccaa actgaattga aggcgcagga gcgcgccttg
     1081 gcgcgattga ggcctgatcg cgcgcaaggc gcgctcctgc atgccccgca caacgaaccc
     1141 catgatcatt ccaaaatatt ccatccgctc ctcgaccatt gccggcgccg gcaagggcct
     1201 gttcctcgat gagccggtgt cgcgcggcgc ggtggtggtg gcgcccgacc gcatcgaccg
     1261 catgatgcac ctgcgcgaac tggagaaatt tccgaaggac tcgatcgaga acatttccag
     1321 cgcgcgctgg ttcgaagact ggttctccat ttcgcccgag tggcccgacg agtgctatgt
     1381 gaaccactcg gacgcgccga acgggctctg gcacctgggc ttcatcttcg cggcgcgcga
     1441 tcttgcggcg ggcgaggaac tcaccgccga ctaccgctac atcctcggca gccacgagcc
     1501 agcgggcttc gccgacagcg caaccggccg cgtggtggtc gggctcgaat ggaaggaaaa
     1561 cgtgcggcgc agcgccgagg ccctgctcag gctgctgtga aatggccgac cgcaccccgg
     1621 tttttcgtgt tattaattat tgtatatatt taaaataata atataattaa taacatcgcc
     1681 aggggcagtg cggtcagctg tcttttgccg tgccggcggt gctacgcgca tcaacttcgt
     1741 gggctgcttg catctggatt ggcacgcggc gcgtaatctc gcttcgacac aacagggagc
     1801 gcgcatgcag ccggcaccgg ctttcggcat caccgtggtc atcgccgtga gcgccaagac
     1861 cggcgcctgg ctgtggcagc gcgcgacacg caacgccggc atggtcgacg cggtgtgggc
     1921 gtggacgctg gcgatgatcg cgctgttcta cgccgccgtg ggcagcgcgc cgccggccac
     1981 gcgcacgcag ctcgcgctga tgggcgcgct gtggggcatg cgcctgggcg tgtacctgtt
     2041 catccgcaat cacggcaagc cggaggactg gcgctacgcg agtttccgcg cgcagtgggg
     2101 cgcaggcgcg gacaggaaga tgttctggtt cttccagttc cagaacgtgt tcaccttgct
     2161 gctgtcggcg agcgcattcc tcactgtcgc ttaccggccc ggcaatgcac cgccggccgc
     2221 gtgctgggcg gcggtcgcgc tgtggctggt ctcggtgctg ggcgaaacgc tggccgaccg
     2281 gcagatgaag cacttccgag ccaacccggc caacaagggc cgcgtgtgcc gcgatggcct
     2341 gtggaactgg tcgcgccatc caaattattt ttttgaatgc gtgcactgga ccgcgtacat
     2401 ctcgctggcc tggggtgcgc cgatgatgtg ggccagcttc gccgcgccgc tggtgatggc
     2461 ctggctgctg ctcaggctgt cgggcgtgcc gatgctggaa aaggaaatga tcgcgcgcaa
     2521 gcccggctac gcggaata
//
LOCUS       DASZZC010000361         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_994124,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000361 DASZZC010000000
VERSION     DASZZC010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994124"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VHE37_07725"
     CDS             complement(<1..593)
                     /locus_tag="VHE37_07725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVT35455.1"
                     /translation="MSKIVDAVTAVLVQDQHVFIAERALHLPAFPGFHAFPGGKVDKT
                     ESEEPFAHAVFAAHPPRLMRAMVRELVEEIGFDLEAAAARGDVLRIDALGIALTPPQA
                     PVRFNTHFFRVMLKTRPSVQLDPGETASYEWASAAELMQRYEHGGLMLAPPTFATLSA
                     LADDLQAARIPGLHFEDRNEELPMTEVLRGVRMIFVRS"
     gene            726..>1597
                     /locus_tag="VHE37_07730"
     CDS             726..>1597
                     /locus_tag="VHE37_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HVT35456.1"
                     /translation="MNLFWIAHGALQRRPLEELPALAQRDDGYLWIDVPACDDQAAQL
                     LRQHLGFHPHGIDEARRRAPIPKIHVYQDHFFCVLHTPELAPQGKLHVFQTALFIHEH
                     RYLVSLHLPAEAGCAPELVWRETDQVRERIEQGRLQPKTPGQLGHAIVSAMGKRLDSC
                     VSELAHQVSRIESSVTRGRLSEYERLLEGLFQVRHNLQAARTIAGTSREVHARMLAFS
                     RGLKDESALWLQDLVDHFDRLKNVCDEEKELLQEVLDLYQTRVANDLSQLVRKLTALG
                     AILVADTLVAGIYG"
BASE COUNT          278 a          509 c          537 g          273 t
ORIGIN      
        1 gagcgcacga agatcatgcg cacgccgcgc agcacctcgg tcatcggcag ctcttcgttg
       61 cggtcctcga aatgcaggcc gggaatgcgc gcggcctgca ggtcgtcggc cagcgccgac
      121 agcgtggcaa aggtgggcgg cgccagcatc aggccgccat gctcgtagcg ctgcatcagc
      181 tcggcggcgg atgcccactc gtagctggcg gtttcgcccg ggtcgagctg caccgagggt
      241 ctggtcttca gcatcacgcg gaagaaatgc gtgttgaagc gcaccggcgc ctgcggcggc
      301 gtcagcgcga tgcccagcgc gtcgatgcgc agcacgtcgc cgcgcgcggc ggcggcctcc
      361 agatcgaaac cgatttcttc caccagctcg cgcaccatgg cgcgcatcag ccgcggcgga
      421 tgcgctgcga acaccgcgtg cgcgaatggt tcttcgctct cggtcttgtc gaccttgccg
      481 ccgggaaagg cgtggaagcc gggaaacgcg ggcaggtgca gtgcgcgctc ggcgatgaac
      541 acatgctggt cctgcaccag cacggcggtg acggcgtcga cgatcttgct catggcgtta
      601 ttttgaccgg tattttttgc cgaccgcagg gctgttttgc atgttaataa ttatagtctt
      661 attattaatt catataatat ttaataacat gcaaaaacgg cttgcggtgg gagcagaatg
      721 cagccatgaa cctgttctgg attgcccacg gcgcgctgca gcggcggccg ctggaagaac
      781 tgcccgcgct ggcgcagcgc gacgacggct acctgtggat cgacgtgccc gcctgcgacg
      841 accaggcggc gcagctgctg cgccagcacc tgggctttca tccgcacggc atcgacgagg
      901 cgcggcggcg cgcgccgatt ccaaagatcc acgtctacca ggatcatttc ttctgcgtgc
      961 tgcacacgcc ggagctggcg ccgcaaggca agctgcacgt gttccagacc gcgctgttca
     1021 tccatgagca tcgttacctg gtatcgctgc acctgccggc ggaagcgggc tgcgcgccgg
     1081 agctggtctg gcgcgagacc gaccaggtgc gcgagcgcat cgagcagggc cgcctgcagc
     1141 ccaagacgcc gggccagctg ggccatgcca tcgtcagcgc catgggcaag cgcctggatt
     1201 cctgcgtcag cgagctggcg caccaggtgt cgcgcatcga gagcagcgtg acacgcgggc
     1261 gcctgtcgga atacgagcgc ctgctcgaag gcctgttcca ggtgcgccac aacctgcagg
     1321 cggcacgcac catcgccggc acctcgcgcg aggtgcatgc gcgcatgctg gccttctcac
     1381 gcggactgaa ggacgagtct gcgctgtggc tgcaggacct ggtcgatcat ttcgaccggc
     1441 tgaagaacgt gtgcgacgag gagaaggaac tgctgcagga agtgctggac ctgtaccaga
     1501 cgcgcgtggc caacgacctg tcgcagctgg tgcgcaagct caccgcgctg ggcgccatcc
     1561 tggtggcgga cacgctggtg gccggcatct acggcat
//
LOCUS       DASZZC010000362         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_478657,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000362 DASZZC010000000
VERSION     DASZZC010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478657"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VHE37_07735"
     CDS             complement(<1..960)
                     /locus_tag="VHE37_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35457.1"
                     /translation="MSSRRRAHALLALCAIALSACSGQLPGSIDRSGGDAVLPAGNKD
                     TEKVCDAYDGPDLSSLHHYEGVEHEHTSYSDGDPHMIPADVFRIAHDKGYSYAANTDH
                     SDNIDDGEYFTLHASCDPSTSSFDPTQEEYCFLNPSVDKLFKWASTQQQAQDASNASF
                     LGIRGFEWTSDVFGHINVFFSQNFSNAKTDGGYALTMDTFWDWFTRDPIMPGLAGSPS
                     SPVPFGGGADGLATFNHPHDKCLFDGVPAPVIDGECDWNDYTLIPAAVERMVGIEAYN
                     DSNRNDRYMPYIVKALDTGWRLSFFAAEDEHFGHYAVETHPKTV"
     gene            complement(1223..1378)
                     /locus_tag="VHE37_07740"
     CDS             complement(1223..1378)
                     /locus_tag="VHE37_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35458.1"
                     /translation="MADGGNNSGVVAIFAIFIIVVVAAFFAWQGGVFGNGKRGADIEV
                     NLPKASH"
     gene            1508..2707
                     /locus_tag="VHE37_07745"
     CDS             1508..2707
                     /locus_tag="VHE37_07745"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT35459.1"
                     /translation="MTSTLRLAALLALLLAGCAGLPLPVATDIPPGFPAALPPIGHVF
                     VIVLENKGYQHSFGLRAHAKYLSRDLPAQGVLLRQYYAIGHESLDNYIAMISGQAPNY
                     ETQADCTLFGFSAFKLRRMHDDGQAEGHGCVYPKDVPNLADQIEASPAKLSWREYAED
                     LDNGSVHSCRHPPVGSKDPDRGSRRSSQYVTRHVPFVYFRSVIDDPARCDRHVVDLQL
                     LTTDLQKVETTPSFSFITPDLCSDGHDGRGYSAQVACDLCADGRPSGYESVDAFLAEW
                     VPRITAAPAFRKDGLLIVTFDEADDEGDESIEEESSASCCNQPLGYNTRIPGIHGPGG
                     GRVGAVLVSPYIKPGTASDVPYNHYALLKSVEELYALKPLGYAGMPGLRSFGSDVYSC
                     PQGTCLR"
     gene            complement(2712..3032)
                     /locus_tag="VHE37_07750"
     CDS             complement(2712..3032)
                     /locus_tag="VHE37_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT35460.1"
                     /translation="MAQIKVVNRAGATSTLDVSNGTSLMDALRDADYDVAGTCGGAMA
                     CGTCHVYVRAGAERLPPKSDDEQMMLEAIGELVTVKAESRLSCQLTASDEITGLEVEI
                     GPIA"
     gene            complement(3097..4137)
                     /locus_tag="VHE37_07755"
     CDS             complement(3097..4137)
                     /locus_tag="VHE37_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVT35461.1"
                     /translation="MTANPQPINLKILLESQTLQRAPFPVPVGWYFIDYSNNLKAGEM
                     RNINVFGQEWVLFRSESGEKVGVTDPYCPHLGAHIGHGGKVCGDHVRCPFHHWEYDTE
                     GWCKNIPYAKVMPPITKKQPILRTLPTVEKYGMIWSFYHPKCEAPTYELPYIPELEDK
                     TGYTGSRRGSWSANTCIQEIAENGVDVAHLRFLHNAPMIPPVEATYDGPRMVLNIGQG
                     YIVGHSYGPGLNVMRFTQKEPTTGKPITATMISYSVPVTHDKTQMNMSFRHIDFPAGT
                     PELAFTKKQIDHMIGAAEGEESAGFESVDFIVWNNKRYRPNPLLCDGDGPIFPFRKWF
                     KQFYVDADPAKI"
     gene            4362..>4711
                     /locus_tag="VHE37_07760"
     CDS             4362..>4711
                     /locus_tag="VHE37_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35462.1"
                     /translation="MLRFLLRSGGVAVLLLLAACGGGSGNPAAAAPAMTAKNLATSLS
                     RPSRLLIGLGSTNASDVKAQHLSIDIFDHYLVGAGDSGWPSWNSPRGAYVGVFAAQAD
                     SLNAVPMYTLYQMA"
BASE COUNT          779 a         1481 c         1569 g          882 t
ORIGIN      
        1 caccgtcttc ggatgcgttt ccaccgcgta gtggccgaag tgctcatcct cggcggcgaa
       61 gaacgacagg cgccaaccgg tgtcgagggc cttgacgatg tacggcatgt agcggtcgtt
      121 gcggttgctg tcgttgtagg cctcgatgcc gaccatgcgc tcgaccgcgg cggggatcag
      181 cgtgtagtcg ttccagtcgc actcgccatc gatcaccggc gccggcacgc cgtcgaacag
      241 gcacttgtcg tgcggatggt tgaaggtggc caggccgtcg gcgccgccgc cgaacggcac
      301 cggcgatgac ggagaaccgg ccagccccgg catgatcggg tcgcgcgtga accagtccca
      361 gaaggtgtcc atggtcagcg cgtagccgcc gtcggtcttg gcgttggaga agttctgcga
      421 gaagaacacg ttgatgtggc cgaacacgtc gctggtccac tcgaagccgc ggatgcccag
      481 gaacgatgca ttgctggcgt cctgcgcctg ctgctgcgtg gaggcccact tgaacagctt
      541 gtcgacgctg ggattgagga agcagtactc ctcctgcgtc ggatcgaagc tgctggtgga
      601 cggatcgcag ctcgcgtgca gcgtgaagta ctcgccgtcg tcgatgttgt cggagtgatc
      661 ggtattggcc gcgtagctgt agcccttgtc atgcgcgatg cggaagacgt cggcgggaat
      721 catgtgcgga tcgccgtcgg agtaggaggt atgttcgtgc tccacgcctt cgtagtgatg
      781 cagcgacgac agatccgggc cgtcgtaggc gtcgcagact ttttccgtgt ccttgttacc
      841 ggccggcagc acggcatcgc cgccgctgcg gtcgatggac ccgggaagct gtccgctgca
      901 ggcggaaagc gcaatggcgc acagggccag cagcgcgtgt gcgcgacggc gggaggacat
      961 gagcgtggac cccgaagatg ttgctgcgat ttcgcaggtg accagcgtag ggcggcacga
     1021 tgacaggcga ttgacccggc gatgggaaaa tcagccccgc cgcaagacct ggttcaatgt
     1081 tattaattat ttgtctatat taaattagtt aaataaataa aaacaccaaa aatgcagatg
     1141 cggtggatgg aaattccgcc gatgcgcaag ggaaatcccg gcgctgcgag gcagggccgg
     1201 gatggtgagt gacacgcctg catcagtgcg aggccttggg caggttgacc tcgatgtcgg
     1261 cgccgcgctt gccgttgccg aacacgccgc cctgccaggc gaaaaatgcc gccaccacca
     1321 cgatgatgaa gatcgcgaag attgcgacca cgccggaatt gttgccgcca tctgccatgt
     1381 ctgttctcct ggagtcttcc tgaagttttc gaccgcccgc gaaaatgccg agttcaggcg
     1441 cgcgccgccg caaaattttc acgcgcggag tgcgctgcgg cgtcgcccgc gaggtctgag
     1501 gaagcaaatg acatccacgc ttcgtcttgc cgcactgctt gccctgctgc tcgccggctg
     1561 cgcgggactg ccgctgccgg tggcgactga catcccgccg ggttttccgg ctgcgctgcc
     1621 gccgatcggc catgtcttcg ttatcgtgct ggagaacaag ggctaccagc acagcttcgg
     1681 cctgcgcgcc catgccaagt acctgagccg cgacctgccg gcgcagggcg tgctgctgcg
     1741 ccagtactac gccatcggcc acgagagcct ggacaactac atcgccatga tcagcggcca
     1801 ggcgcccaac tacgagacgc aggccgactg cacgctgttc gggttctccg cgttcaagct
     1861 caggcgcatg cacgacgatg gccaggcgga agggcatggc tgcgtctacc cgaaagacgt
     1921 gccgaacctc gccgaccaga tcgaggcctc gccggcaaaa ctgtcgtggc gcgagtacgc
     1981 cgaggacctc gacaacggca gcgtgcacag ctgccgccac ccgccggtgg gcagcaagga
     2041 cccggaccgc ggctcgcggc gcagcagcca gtacgtcacg cggcacgtgc ccttcgtcta
     2101 tttccgctcg gtgatcgatg acccggcgcg ctgtgatcgc cacgtggtcg acctgcagct
     2161 gctgaccacg gatctgcaga aggtcgagac cacgccaagc ttcagtttca tcacgccgga
     2221 cctgtgcagc gacggccacg acggccgcgg ctactcggcg caggtggcct gcgatctgtg
     2281 cgccgacggt cgcccaagcg gctatgaatc cgtcgacgcc ttcctggccg agtgggtgcc
     2341 gcgcatcacg gcggcgccgg cgttccgcaa ggacggcctg ctgatcgtca ctttcgacga
     2401 ggccgacgac gagggcgatg aatcgatcga ggaagaatcg tcggccagtt gctgcaacca
     2461 gccgctgggt tacaacacgc gcatcccggg tatccacggg ccgggcggcg gccgcgtcgg
     2521 cgcggtgctg gtgtcgccgt acatcaagcc gggcacggcc agtgacgtgc cctacaacca
     2581 ctacgcgctg ctgaagagcg tcgaagagct gtatgcgctg aagccgctgg gctatgcggg
     2641 catgccgggg ctgcgcagtt tcggcagcga cgtgtacagc tgtccgcagg gcacctgcct
     2701 gcgctgagcc gtcaggcgat cggcccgatc tccacttcca gtcccgtgat ttcgtcgctg
     2761 gctgtgagct ggcatgacag gcgcgactcg gctttcaccg tgaccagttc gccgatggct
     2821 tccagcatca tctgctcgtc gtcggatttc ggcggcagcc gctcggcgcc cgcgcgcacg
     2881 tacacgtggc aggtgccgca ggccatggcg ccgccgcagg tgccggcgac gtcgtagtcg
     2941 gcatcgcgca gcgcgtccat cagcgatgtg ccgttgctga cgtcgagcgt gctggtcgcg
     3001 cccgcgcgat tgacgacttt gatctgcgcc atgctgttcc tgccttgcca agaaaaatgc
     3061 cgggccgcag gtgcgcggcc cggcatgatg tgccgctcag atcttcgccg gatcggcgtc
     3121 gacgtaaaac tgcttgaacc acttgcggaa cgggaagatc gggccgtcgc cgtcgcacag
     3181 cagcgggttg gggcggtagc gcttgttgtt ccagacgatg aagtccaccg actcgaagcc
     3241 cgcgctctct tcgccttcgg ccgcgccgat catgtgatcg atctgcttct tggtgaatgc
     3301 cagttccggg gtgcccgccg ggaaatcgat gtggcggaac gacatgttca tctgcgtctt
     3361 gtcatgcgtc accggcaccg agtacgagat catggtcgcg gtgatcggct tgccggtggt
     3421 cggctccttc tgggtgaagc gcatgacgtt gaggccgggg ccgtagctgt ggccgacgat
     3481 gtagccctgg ccgatgttca gcaccatgcg cgggccgtca taggtggctt cgaccggcgg
     3541 aatcatcggc gcgttgtgca ggaagcgcag gtgcgccacg tcgacgccgt tctcggcgat
     3601 ttcctggatg caggtgttgg cgctccagct gccgcggcgc gagccggtgt agccggtctt
     3661 gtcttccagc tcggggatgt acggcagctc ataggtcggc gcttcgcact tggggtggta
     3721 gaacgaccag atcatcccgt acttctccac cgtgggcagc gtgcgcagga tcggctgctt
     3781 cttggtgatg ggcggcatca ccttggcgta gggaatgttc ttgcaccagc cctcggtgtc
     3841 gtattcccaa tggtggaacg ggcagcgcac gtggtcgccg cagaccttgc cgccgtggcc
     3901 gatgtgcgcg cccaggtgcg gacaatacgg gtcggtcacg ccaaccttct cgccgctctc
     3961 gctgcggaac agcacccact cctggccgaa gacgttgatg ttgcgcatct cgccggcctt
     4021 gaggttgttc gagtagtcga tgaagtacca gcccaccggc accgggaacg gcgcgcgctg
     4081 cagcgtctgg gattcgagca ggatcttcag gttgatcggt tggggattcg cagtcatcgc
     4141 aacgtctccg gtctcaatgc gggcgcgacc atcgcgctac tcggcagcgt ggactttgcg
     4201 cgcgcgcgaa accaatttct tcccaaggat gcgtcttgca atcggcctcg ttttgaggca
     4261 tgcggagaag tccgtcgcgg cgctgccgcc attcatcggc agcggcgtgc gcgcggcctt
     4321 gcgcacccta tgctgccggc tgattttctg cggcctgaac catgcttcgt ttcctgttgc
     4381 gcagcggcgg tgtcgccgtg ttgctgttgc tcgcagcctg cggcggcggt agcggcaatc
     4441 cagccgctgc tgcgccggcg atgacggcga agaatctcgc cacctcgctc agccggccgt
     4501 cgcgcctgct catcggcctg ggcagcacca acgcgtccga cgtgaaggcg cagcacctgt
     4561 ccatcgacat cttcgaccac tacctggtcg gcgccggcga ttccggctgg cccagctgga
     4621 acagcccgcg cggcgcgtac gtgggcgtgt tcgcggcgca ggccgattcg ctcaacgcgg
     4681 tgccgatgta cacgctgtac cagatggcca g
//
LOCUS       DASZZC010000363         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_858997,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000363 DASZZC010000000
VERSION     DASZZC010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858997"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VHE37_07765"
     CDS             <1..934
                     /locus_tag="VHE37_07765"
                     /inference="COORDINATES: protein motif:HMM:NF005537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HVT35463.1"
                     /translation="ARLPGGACAAGEHRVNPLIAALPGSTAFAAALRQHAALESLHID
                     LHRFPDGECLVRLDGELRGRDLLLVCSLNAPDDKLLPLLFAASTARDLGAQRVILVAP
                     YLAYMRQDRRFHAGEAISSRPFAALLSAAFDGLVTVDPHLHRYRNLRELYSIPARAVA
                     AAPDLAGWIVREAPSPLIVGPDEESAQWAQDVAARAAAPCIVLRKTRLGDREVSIDPP
                     PPGMHAGRTPVLIDDIISTGRTLLQAARVLRAQGYAAPVCVGVHALCPAPAYEELRAG
                     VARVVSCNTVEHASNAIDVAPAVWRAAQEMLGPD"
     gene            complement(1063..3357)
                     /locus_tag="VHE37_07770"
     CDS             complement(1063..3357)
                     /locus_tag="VHE37_07770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT35464.1"
                     /translation="MGTRNLAALQTPRSVVLVGEPRGALQQRLAENLLACTGPQRPLH
                     VCAALPWPAADLAVIVDAALATPATVEAFAQAGGRALLWAADSAVPEEFLRACRPARV
                     RVLGPRSCGAAHAASGFNATWLEQAPLPGRVALIAQSRSVAAAVLDFALGRRLGFSWL
                     AVSGGEADVDVADLLDQAALDPHTRAVVLELRKIGQARKFMSAARAAARIKPVVVLQS
                     PRAGDSGPDPVRSAAFARAGLVECDDLNGLFHALAALERLPPTPQYRVALAGNGAGLC
                     ALGSAALARHGVAAATISEASRAAIGALNAQARFSSGSADLGEMDAAAIVAALRVLLA
                     DGGADLVMLVHGPGTTCSPHTLAQALAQAQIGPRLLAVWPGLEQAGTARTLSAAAGIA
                     TFATAEQAAQAVRDRLLHDLNRQLLTQTPPADPPGTVDAAACTAQLRDHLDAGDLPQA
                     SAAAALRAYGLHGARPAAAGEHFDAALECHAELGMQLSLQLEARHGSAPRAYALPPLD
                     PLLAARMLADAGVRPGPDHDALSHALIRLGRMAIEQPLLRVARLRLQVQDGAAVVLPG
                     LQLRAAPPLPERLRLALAPYPSQVEHVAPLGGGVRYRVRAIRPSDEPALIGLLQRLDP
                     DEVRYRFFIHLRHFSHDMAARMTQIDYDRELALVAFVEGHEQEVVGTGTLIADADGRE
                     AEFALLVHHAHARRGLGRHLLTRLLEHARAQGIGTVYGDVLADNRSMLTLAQRLGFSS
                     KPDPLDPGCRRVEISLAGGSPHPP"
     gene            complement(3359..>3987)
                     /locus_tag="VHE37_07775"
     CDS             complement(3359..>3987)
                     /locus_tag="VHE37_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HVT35465.1"
                     /translation="AGLLAVDLVMSNRAQLAFCAVRPPGHHAERAQAMGFCFFNNVAV
                     AAAEALARGAKRVAILDFDVHYGNGTADIFRAEPRVTLLSTYQHPLYPYWQGAENAPH
                     LVDVALPPYAGSTAFRAAVTERWLPVLERERPDLLLVSAGFDAHRDDGMAQLRLATED
                     YRWLGMVIQQVALECCGGRVVATLEGGYNLDALARSVEAFIAPFLGGL"
BASE COUNT          575 a         1438 c         1422 g          552 t
ORIGIN      
        1 cgctcgacta cctggcggtg catgcgccgc cggtgaacat cgcgtgaatc cgctgatcgc
       61 cgcattgccc ggcagcactg cgttcgcggc cgccttgcgc cagcacgccg cgctggagtc
      121 gctgcacatc gacctgcacc ggtttccgga cggcgaatgc ctggtgcgtc tcgacggcga
      181 actgcgcggg cgcgacctgc tgctggtgtg ctcgctcaac gcgcccgacg acaagctgct
      241 gccgttgctg ttcgccgcat cgaccgcgcg cgatctcggc gcgcagcgcg tgatcctcgt
      301 cgcgccgtat ctcgcctaca tgcgccagga ccggcgcttc catgccggcg aggcgatcag
      361 ttcgcggccg ttcgccgcgt tgctgtcagc ggcgttcgat ggcctggtga cggtggaccc
      421 gcacctgcac cgctaccgca acctgcgtga gctgtactcg atcccggcgc gcgcggtggc
      481 ggcggcgccc gatctcgccg ggtggatcgt gcgcgaagcg ccatcaccgc tgatcgtcgg
      541 ccccgacgaa gaaagcgcgc agtgggcgca ggatgttgcc gcgcgcgccg ccgcgccgtg
      601 catcgtgctg cgcaagacgc ggctgggcga ccgcgaggtg agcatcgatc cgccgccgcc
      661 cggcatgcac gcaggacgca cgccggtgct gatcgacgac atcatttcca ccggccgcac
      721 gctgctgcag gccgcgcgcg tgctgcgcgc gcagggctac gccgcgccgg tgtgcgtggg
      781 cgtgcacgcg ctgtgcccgg cgccggccta tgaggaactg cgcgccggcg tcgcgcgcgt
      841 ggtcagctgc aacacggtgg agcatgcgag caatgccatc gacgtggcgc ccgcggtgtg
      901 gcgcgcggcg caggaaatgc tcgggccgga ctagcgtgcc gacgtttcgt tgcgctcgtt
      961 ggcgcatcga tttttccgtc ggttttacct ccaccgcacc ggcattttcg atgttataaa
     1021 ttattttata tattaaaata aatacgatat ttaataacaa catcaaggcg ggtgcggtga
     1081 tcctcctgcg agcgatattt ccaccctgcg gcagccggga tcgagcggat cgggtttgct
     1141 gctgaagccc agccgctgcg ccagcgtcag catgctgcgg ttgtccgcca gcacgtcgcc
     1201 gtacaccgtg ccgatgccct gcgcgcgcgc atgttcgagc agccgcgtga gcaggtggcg
     1261 gccgagaccg cggcgcgcgt gcgcgtggtg caccagcagc gcgaactcgg cctcgcgtcc
     1321 gtcggcatcg gcgatcagcg tgccggtgcc gaccacttcc tgttcatggc cttcgacgaa
     1381 ggcgaccagc gccagctcgc ggtcgtaatc gatctgcgtc atgcgcgcgg ccatgtcgtg
     1441 gctgaagtgg cgcaggtgga tgaagaagcg gtagcgcact tcgtcggggt cgagccgctg
     1501 cagcaggccg atcagcgccg gttcgtccga cggccggatg gcgcgcacgc ggtagcgcac
     1561 gccgccgccg agcggcgcca catgttccac ctgcgacgga taaggcgcca gcgccagccg
     1621 caggcgctcc ggcaacggcg gcgccgcacg cagttgcagc ccgggcagca cgacggcggc
     1681 gccatcctgc acctgcagcc gcagcctggc cacgcgcagc agcggctgct cgatggccat
     1741 gcgcccaagc cggatcagcg cgtgcgacag cgcatcatgg tctggaccgg ggcgcacgcc
     1801 ggcgtcagcc agcatgcgcg ccgccagcag cgggtccagc ggcggcagcg cgtaggcccg
     1861 cggcgcgctg ccatgcctgg cctcgagttg cagcgacagc tgcatgccca gttcggcgtg
     1921 gcactccagc gcagcatcga agtgttcgcc ggcggcggcc ggccgcgcgc cgtgcaggcc
     1981 atacgcacgc aacgccgccg cggcgctggc ctgaggcagg tcgccggcat caagatggtc
     2041 acgcagctgc gccgtgcacg cggcggcatc gacggtgccg ggcggatcgg ccggcggcgt
     2101 ctgcgtcagc aactggcggt tgagatcgtg cagcaggcgg tcgcgcaccg cctgcgcggc
     2161 ctgctcggcg gtggcgaagg tggcgatgcc agccgcggca ctgagcgtgc gcgccgtccc
     2221 ggcctgctcc agccccggcc acaccgcgag caggcgcggg ccgatctgcg cctgcgccag
     2281 cgcctgtgcc agtgtgtgcg gactgcaggt ggtgcccggt ccgtgcacca gcatgaccag
     2341 gtcggcgccg ccgtcggcca gcagcacgcg cagcgcggcg acgatggctg cggcatccat
     2401 ctcgcccagg tcggcgctgc cggaggaaaa acgcgcctgc gcattgagcg cgccgatggc
     2461 cgcgcggctt gcttcggaaa tcgtagcagc ggcaacgccg tggcgcgcca gcgcggcgct
     2521 gcccagcgcg cacaggccgg cgccgttgcc agccagcgcc acacggtact gcggcgtggg
     2581 cggcagccgt tcaagcgcgg ccagcgcatg gaacagcccg ttgaggtcat cgcactccac
     2641 cagcccggcg cgggcgaaag cggccgagcg caccgggtcg ggcccgctgt cgccggcgcg
     2701 cgggctctgc agcaccacca ccggcttgat gcgcgcggcg gcgcgtgcgg ccgacatgaa
     2761 cttgcgcgcc tgcccgatct tgcgcagttc gagcaccacc gcgcgggtgt gcggatcgag
     2821 cgcggcctgg tcgagcaggt cggccacgtc gacatcggcc tcgccgccgc tcaccgccag
     2881 ccaggagaat cccaggcgcc ggccgagcgc gaaatccagc accgcggccg ccaccgagcg
     2941 cgattgcgcg atcagcgcca cgcggccggg cagcggcgcc tgctccagcc aggtggcatt
     3001 gaagcccgat gccgcatgcg ccgcgccgca gctgcgcggg cccagcacgc gcacgcgggc
     3061 cgggcggcag gcgcgcagga attcctccgg caccgcgctg tcggcggccc acagcagcgc
     3121 gcggccgccg gcctgcgcaa atgcttcgac cgtggcgggt gtggccagcg cggcatcgac
     3181 gatcacggcg agatcggcgg ccggccaggg cagtgcggcg caaacatgca gcgggcgctg
     3241 tggccccgtg caggccagca ggttttccgc cagccgctgc tgcagggcgc cgcgcggttc
     3301 tcccaccagc accacgctgc gcggcgtctg cagcgccgcc aggttacgcg tgcccacgct
     3361 acagtccgcc gagaaacggt gcgatgaacg cttcaacgct gcgcgccagc gcgtcgaggt
     3421 tgtagccgcc ctcgagcgtg gccaccacgc ggccgccgca gcattccagc gccacctgct
     3481 ggatcaccat gcccagccag cggtagtcct cggtggccag gcgcagctgc gccatgccgt
     3541 cgtcgcggtg cgcgtcgaaa ccggccgaga ccagcagcag gtcgggacgc tcgcgttcca
     3601 gcaccggcag ccagcgctcg gtgacggctg cgcggaacgc cgtgctgccg gcgtacggcg
     3661 gcaacgccac gtcgaccagg tgcggcgcat tctccgcgcc ctgccagtac gggtagagcg
     3721 gatgctggta ggtggacagc agcgtcaccc gcggctcggc gcggaaaatg tcggcggtgc
     3781 cgttgccgta atgcacgtcg aaatcgagga tggccacgcg cttcgcgccg cgcgccagcg
     3841 cctcggccgc cgccaccgcc acgttgttga agaagcagaa acccatggcc tgcgcgcgtt
     3901 cggcgtggtg gcccggcgga cgcaccgcgc agaaggccag ttgcgcccgg ttgctcatca
     3961 ccaggtccac tgccagcaaa ccggcgc
//
LOCUS       DASZZC010000364         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1248234,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000364 DASZZC010000000
VERSION     DASZZC010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248234"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /gene="motD"
                     /locus_tag="VHE37_07780"
     CDS             complement(<1..887)
                     /gene="motD"
                     /locus_tag="VHE37_07780"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HVT35466.1"
                     /translation="MAKHKHEEHQNHEAWAIPYGDLVTLLLALFVVMYAVSSVNEGKF
                     RVLAESMAEAFGGAPRSIAPIQVGEKISKGSDNSQKMSVLPSPSLPQALGGVMRSLSN
                     PRVLDGNLRSTIAQHQLDRSGNTGYKHGLENLQHMGDQIRSAMGELIGKQLVIVRQTE
                     LWLEVEIKTDILFPSGVARVDDNAVPVLTRLAGIMQPFPNSIRVEGYTDNVPISTYTY
                     PSNWELPAARAASVVRLFIDHGVDPKRMSVAGLGEYRPVADNDTPTGRNRNRRVVLVV
                     LADNGGSEPQAINAALQPG"
     gene            complement(897..1637)
                     /locus_tag="VHE37_07785"
     CDS             complement(897..1637)
                     /locus_tag="VHE37_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HVT35467.1"
                     /translation="MDLLSIVGFVMAFVVLIVGSILKGSGVEALWGPAAFVIVICGTI
                     SSILLQTPMATFKHAMKMAKWVFSPPRHDNTALIGKVVEWSNAARKQGLLALEASVEQ
                     ESDPFIKKGLQLLVDGAEPDMIRNVLEVEMGTHEHFNLQGAKVFEGMGIYAPTLGIIG
                     AVMGLMAVMRNLADPSKLGHGIAAAFVATIYGIASANMFLLPMASKLKSIVADQTRTR
                     EMLIEGLISISRGDNPRNIEAKLQGFFH"
     gene            complement(1641..>1965)
                     /locus_tag="VHE37_07790"
     CDS             complement(1641..>1965)
                     /locus_tag="VHE37_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVT35468.1"
                     /translation="DPSKLRRLDRWDMVMVRGQPLRLVHLDRWVGAPMPADMPRHIVV
                     AQVGSEQYGFVVREVRGREEVVIKPLCPLLRGLSGLAGATVTGEGRVALILDFAGLVA
                     AAARS"
BASE COUNT          298 a          682 c          645 g          340 t
ORIGIN      
        1 tctcccggct gcagcgcggc gttgatcgcc tgcggctcgc tgccgccgtt gtcggccagc
       61 accaccagca ccacgcggcg gttgcggttg cgcccggtcg gcgtgtcgtt gtcggccacc
      121 gggcggtact cgcccaggcc ggccacgctc atgcgttttg gatcgacacc atggtcgatg
      181 aacagccgca ccacgctggc cgcgcgcgcc gccggcagct cccagttgga cggataggtg
      241 taggtgctga tcggcacgtt gtcggtgtaa ccctcgacgc ggatcgagtt ggggaacggc
      301 tgcatgatgc cggccagccg cgtcagcacc ggcaccgcgt tgtcgtccac ccgcgccacg
      361 ccgctgggga acaggatgtc ggtcttgatc tccacctcga gccacagctc ggtctggcgc
      421 acgatcacca gctgcttgcc gatcagctcg cccatggcgc tgcggatctg gtcgcccatg
      481 tgctgcaggt tttccagccc gtgcttgtag ccggtgttgc cgctgcggtc gagctggtgc
      541 tgcgcgatgg tggagcgcag gttgccgtcg agcacgcgcg ggttgctcag cgagcgcatc
      601 acgccgccca gcgcctgcgg cagtgacggc gacggcagca cgctcatctt ctgcgagttg
      661 tcggaaccct tgctgatctt ctcgcccacc tggatcggcg cgatggagcg cggcgcgccg
      721 ccgaaggcct ccgccatcga ttcggccagc acgcggaact tgccctcgtt gacgctggac
      781 accgcgtaca tcaccacgaa cagcgcgagc agcaacgtca ctaggtcgcc gtagggtatg
      841 gcccatgcct cgtggttctg atgttcttcg tgcttgtgct tggccatggt gcgcggtcag
      901 tggaagaagc cctgcagctt ggcctcgatg ttgcgcgggt tgtcgccgcg cgaaatggaa
      961 atcagcccct cgatcagcat ctcgcgcgtg cgcgtctggt cggcgacgat gctcttgagc
     1021 ttgcttgcca tcggcagcag gaacatgttg gccgaggcga tgccgtaaat ggtggcgacg
     1081 aacgccgcgg cgatgccgtg gccgagcttg gacggatcgg ccaggttgcg catcaccgcc
     1141 atcaggccca tcaccgcgcc gatgatgccc agcgtgggcg cgtagatgcc catgccctcg
     1201 aacaccttgg cgccctgcag gttgaaatgc tcgtgcgtac ccatctccac ctcgagcacg
     1261 ttgcggatca tgtcgggctc ggcgccgtcg accagcagct gcaggccctt cttgatgaac
     1321 ggatcgctct cctgctccac gctggcttcc agcgccagca ggccctgctt gcgcgcggcg
     1381 ttgctccact ccaccacctt gccgatcagc gcggtgttgt cgtgcctggg cgggctgaac
     1441 acccacttgg ccatcttcat cgcgtgcttg aaggtggcca tcggcgtctg cagcaggatc
     1501 gacgagatgg tgccgcagat gacgatgacg aacgccgccg ggccccacag cgcctcgacg
     1561 ccgctgccct tgaggatgga gccgacgatc agcaccacga aggccatgac gaagccgacg
     1621 atggaaagca ggtccatggc tcagctccgt gccgccgcgg ccaccaggcc agcgaaatcg
     1681 aggatcagcg ccacgcggcc ttcgccggtg acggtggcgc cggccaggcc ggacagtccg
     1741 cgcagcagcg ggcacagcgg cttgatcacc acttcctcgc ggccgcgcac ctcgcgcacc
     1801 acgaagccgt actgctcgct gcccacctgc gccaccacga tgtggcgcgg catgtcggcc
     1861 ggcatcggcg cgccgaccca gcggtccagg tgcaccaggc gcagcggctg gccgcgcacc
     1921 atcaccatgt cccagcggtc gagccggcgc agcttggatg gatcc
//
LOCUS       DASZZC010000365         8557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_859253,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000365 DASZZC010000000
VERSION     DASZZC010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859253"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VHE37_07795"
     CDS             complement(<1..1422)
                     /locus_tag="VHE37_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HVT35469.1"
                     /translation="MARRGSKAPPSPLPDQPPDWRVLRTLWPYLTEFPLRVILAMLFL
                     VAAKIAGVVLPLMLKKLVDGLDIARGNVAAVPLALLLGYGAMRFANVIFGELRDVVFG
                     RVAERAMRRSALDVFRHLHQLDLEFHLTRRTGGLSRDIERGVAGISFLLRFMLFNIIP
                     TLLEIGMVAAILLVKYGSEFAVIIGGAVGLYIFLSVAITEWRTKYVREANQLDSRAQT
                     RAVDSLLNYETVKYFGNEQHEAAEYDKFMSQWERAQQQQRLSLSALNGVQALIVALSL
                     TWLMILASKKVAGGTMTLGDFTAVNAYIIQLFIPLNFLGFVYREIRRALTDMSRMFGL
                     QQQKARIADAPGAQVLAAGTATVEFEHVRFAYGERAILHDVSFAIPAGKKLAVVGPSG
                     AGKSTLARLLFRFYDAAGGAVKINGIDIRRLTQASLRAHIGVVPQDTVLFNDTIYYNI
                     AYGRTGAAREDVERAARLAPLPRG"
     gene            complement(1568..3073)
                     /gene="nuoN"
                     /locus_tag="VHE37_07800"
     CDS             complement(1568..3073)
                     /gene="nuoN"
                     /locus_tag="VHE37_07800"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HVT35470.1"
                     /translation="MKMTFAQWLALSPILIISLTIVVVMMAIAIKRHHWWNATFAVAG
                     LNAALLACSVLFFGPLMFPGVVPQYLPQSITPLVVIDSYSIFYMGLILSATLATATLS
                     HAYIDGYAGNKEEIYLLLMLSSLGAILLACSKHFASLFLGLELLSIPLYGMVAYPLKN
                     RHPLEAGVKYLVLSAAASAFLLFGMALIYARTGTLAFAELAPRMNGLHVTRDLYMLTG
                     GALLIAGLAFKLSLVPFHLWTPDVYEGAPAPVTSYLATASKTAVFAVLLRWFVQGGAD
                     RQGLFLDVLALLAIASILVGNVLALQQTNIKRMLAYSSIAHLGYCLVAVVAGGPLAVE
                     AVGAYLLTYVVTTLGAFGVVTLMSSPIGDNDADEIDDYRGLFWTRPFLSSILTVAMLS
                     LAGIPLTAGFIGKFYITAVGVDARLWVLLGAVVIGSAIGLYYYLRLMIALYNFDAREA
                     ARRRRFFDADTNWAQQAGGYMVLLLMLLMMILGMYPEPFLLVLRAANMTAF"
     gene            complement(3070..4602)
                     /gene="nuoM"
                     /locus_tag="VHE37_07805"
     CDS             complement(3070..4602)
                     /gene="nuoM"
                     /locus_tag="VHE37_07805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVT35471.1"
                     /translation="MILIALILIPFVAGLACWLGERTMGVQFPRWIALGGMLISLGIS
                     IYLWVTCDWSLAGLGTAHPQWTIEYRGEWIPRLGISFHFAMDGFALLLVTLTNLLGVA
                     AVACSWREIQRNVGFFHLNLLWNLGGVVGVFLTLDLFLFFFLWEMMLVPMYFLIALWG
                     HASPGSKGTIYAATKFFIFTQASGLLMLLSILGLVFINYHATGTLTFDYEDLLKAKLS
                     TTTAFWLMLGFFVAFAVKTPAVPLHSWLPDAHSQAPTAGSVDLAGILLKTAAFGMIRF
                     GIPLFPAASASFAPVAMWLGIAGIIYGGVMTFAQTDIKRLVAFSSISHMGFVLVGIYA
                     GTPQALQGVVMQMLAHGISAGALFILCGEIYERLHTRDLTQMGGLWANFPYLPPIALF
                     FAAASLGLPGLGNFVGEFLILLGTWPVNHTVTIFAASGLILAAVYSLMMVQRAFHGAP
                     NQSLGDHLEDLSKRELVLMMSLMAILLWLGLYPQTVLNTSAASMQAVQQVYAAAHAAV
                     AP"
     gene            complement(4607..6460)
                     /gene="nuoL"
                     /locus_tag="VHE37_07810"
     CDS             complement(4607..6460)
                     /gene="nuoL"
                     /locus_tag="VHE37_07810"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVT35472.1"
                     /translation="MSLLFLVFVFPLLGFVTLAFARSRMPENAAALVGVGSIGLSFLT
                     TIIAGWQFLHQPEPAAYTQLLWTWMSVPGFAPQFALRLDALSLTMMFVITGVGFFIHL
                     FASWYMRGDASYDRFFSYMNLFVASMLFLVLGDNLLFLYFGWEGVGLSSYLLIGFWYQ
                     DPANGAAARKAFVVTRVGDTFMAVGLFLIFHQLGTLNIDEAMARAAQHWGVGSDVATL
                     AALLLLGGAVGKSAQLPLQTWLPDAMAGPTPVSALIHAATMVTAGVYLIARTHVIFEL
                     SPFALECVGWVGALTALLASFTALTQTDIKRVLAYSTMSQIGYMFLGLGVGAYQPAIF
                     HLMTHAFFKALLFLSAGSVILAMHHEQDIFKMGGLRKQLPFTYTVFLIGCLGLIACPP
                     FSGFFSKDEILFEAYASGHLELWALGLFGAYLTAVYTFRLVFIAFFGESHAHGDHAPH
                     QPHGFDHSLPLGVLAFFAVAGGFIPLHLESVLPPLPISEAAEHAKEKLAALSVIVSGL
                     GVLTAYVLFIKDPRRLKAITHNEIVEPFTHWARAAFGFDRLYDIVFKAPFLWFVRVNR
                     NDAIDVAIGQIPVFLTWLHGRFARTESGKLRWYAAAMGAGAAAVLAVVLWT"
     gene            complement(6465..6782)
                     /gene="nuoK"
                     /locus_tag="VHE37_07815"
     CDS             complement(6465..6782)
                     /gene="nuoK"
                     /locus_tag="VHE37_07815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVT35473.1"
                     /translation="MTNTLAVPMEHGLLLAAVLFALGFCGLLVRRNILYMLMSIEIMM
                     NAAALAFVTAGARWGNPDGQVMFILILTLAASEASVGLALLLQLYRRFQTLDIDAASE
                     MKG"
     gene            complement(6775..7284)
                     /gene="nuoJ"
                     /locus_tag="VHE37_07820"
     CDS             complement(6775..7284)
                     /gene="nuoJ"
                     /locus_tag="VHE37_07820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVT35474.1"
                     /translation="MEQFLAYFFNPFYWAAFIAVFSTVMVISSANPVHALLYLVLSLL
                     SVAMIFFTLGAPFAGLLEVIVYAGAIMVLFIFVVMMLNLGSKVTEQERRWMNLRAWIG
                     PMILSAVLLAELLYMLPSNGLHMAGLQEVGAPRVGIALYGPYLLAVEFGSMLLLGALV
                     AAYHLGKHD"
     gene            complement(7284..7826)
                     /gene="nuoI"
                     /locus_tag="VHE37_07825"
     CDS             complement(7284..7826)
                     /gene="nuoI"
                     /locus_tag="VHE37_07825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HVT35475.1"
                     /translation="MSLKSSLAGIWSQFRSIGIIFSHSWRKRDTIQYPEVKPYVPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVACISLQKAEKPDGRWYPEFFRINFSRCIFC
                     GLCEEACPTNAIQLTPDYEMCEYNRQNLVYEKEDLQIAGTGKYHEYNFYRQAGMAIAG
                     KPKGAAANEVPPVDVKGLMP"
     gene            complement(7829..>8557)
                     /locus_tag="VHE37_07830"
     CDS             complement(7829..>8557)
                     /locus_tag="VHE37_07830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HVT35476.1"
                     /translation="MSMMLIAFALVPLSNTLWVADLNLGILYFFAIAGLAVYAVLLAG
                     WSSNSKYSLIGGVRVTALSITYEVFMGLSLLGVVMLAGSFNLREIVLDQAREGWLWWR
                     QPLGMCLFALAGVAATHRTPFDLPEAEQELAAGFHTEYSGMKFGMFFVGEYVGIVLVS
                     GLMVSLFFGGWLGPGVERWAWLGFFWFAFKNLIFMSSFILIRAALPRPRYDQLMDAGW
                     KICLPLALINTLGTAAWMLAKGHA"
BASE COUNT         1759 a         2695 c         2871 g         1232 t
ORIGIN      
        1 cccgcgagga agtggagcga gccgcgcggc gcgctccaca tcctcgcggg cggcgccggt
       61 acgaccgtag gcgatgttgt agtagatggt gtcgttgaac agcacggtgt cctgcggcac
      121 cacgccgatg tgcgcgcgca gcgaggcctg cgtcagccgg cggatatcga tgccgttgat
      181 cttcaccgcg ccgccggccg cgtcgtagaa gcggaacagc aggcgcgcca gcgtcgactt
      241 gccggcgccg ctggggccga ccaccgcgag cttcttgccc gcgggaatgg cgaaggagac
      301 gtcgtgcagg atggcgcgct cgccgtaggc gaagcgcacg tgctcgaact ccaccgtggc
      361 cgtacccgcc gccagcacct gcgcgccggg cgcatcggcg atgcgcgcct tctgctgctg
      421 caggccgaac atgcgcgaca tgtcggtcag cgcgcggcgg atctcgcggt agacgaagcc
      481 gaggaagttg agcgggatga acagctggat gatgtaagcg ttgaccgcgg tgaaatcgcc
      541 cagcgtcatc gtgccgcctg ctactttttt cgaagcaagg atcatcagcc aggtcagcga
      601 cagcgccacg atcagcgcct gcaccccgtt gagcgccgac agcgacaggc gctgctgctg
      661 ctgcgcgcgt tcccactggc tcatgaactt gtcgtactcg gccgcctcgt gctgctcgtt
      721 gccgaaatac ttcaccgttt cgtagttgag cagcgaatcg accgcgcgcg tctgcgcgcg
      781 cgagtcgagc tggttggcct cgcgcacgta cttggtccgc cactcggtaa tggccaccga
      841 caggaaaatg tacagcccca ctgcgccgcc gatgatcacg gcgaactcgc tgccgtattt
      901 caccagcagg atggcggcga ccatgccgat ctcgagcagc gtcgggatga tgttgaacag
      961 catgaaacgc agcaggaagc tgatgccggc cacgccgcgc tcgatgtcgc gcgacagccc
     1021 gcccgtgcgc cgggtgagat gaaattccag gtcgagctgg tgcaggtggc ggaacacgtc
     1081 gagtgcgctg cgccgcatgg cgcgctcggc cacgcggccg aacaccacgt cgcgcagctc
     1141 gccgaagatg acgttggcga agcgcatggc gccgtagccc agcagcagcg ccagcggcac
     1201 cgcggcgacg ttgccgcggg cgatgtcgag gccatcgacc agcttcttca gcatcagcgg
     1261 cagcaccacg ccggcaatct tggccgcgac caggaacagc atggccagga tcacgcgcag
     1321 cggaaactcg gtgaggtatg gccagagcgt gcgcagcacg cgccagtcgg gcggctggtc
     1381 gggcaggggc gaagggggcg ctttggaacc gcggcgggcc atgtgcgatg gagaaaactg
     1441 cttcgatttg gcctgaccgc actgccatct ggcttgttac aaaatattat ctaatttgat
     1501 ttttagataa taattaataa catgcaaaaa ggcggtgcgg tcagcgtgtt tcaggcccgc
     1561 ggcgcgttca gaacgcggtc atgttggccg cgcgcagcac cagcaggaac ggctcggggt
     1621 acatgcccag gatcatcatc agcagcatca gcagcagcac catgtagccg ccggcctgct
     1681 gcgcccagtt ggtgtcggcg tcgaagaagc gccggcggcg cgcggcttcg cgcgcatcga
     1741 agttgtacag cgcgatcatc aggcgcaggt agtagtagag accgatcgcg ctgccgatca
     1801 ccacggcgcc gagcaggacc cacaggcgcg catccacacc gaccgcggtg atgtagaact
     1861 tgccgatgaa gccggcggtg agcggaatgc cagccagcga cagcatggcc acggtgagga
     1921 tcgacgacag gaacgggcga gtccagaaca ggccgcggta gtcgtcgatc tcgtcggcat
     1981 cgttgtcgcc gatgggactc gacatcagcg tgaccacgcc gaacgctccg agcgtggtca
     2041 ccacgtaggt cagcaggtat gcgccgacgg cttccaccgc cagcgggccg ccggcgacca
     2101 ccgcgaccag gcagtagccc aggtgcgcga tcgacgagta cgccagcatg cgcttgatgt
     2161 tggtctgctg cagcgccagc acgttgccga cgaggatgga cgcgatggcc agcagcgcga
     2221 gcacgtcgag gaacaggcct tgccggtcgg cgccgccctg cacgaaccag cgcagcagca
     2281 cggcgaacac cgcggtcttg ctggcggtgg cgagataact ggtcaccggc gcgggcgcac
     2341 cttcgtagac gtcgggcgtc cacaggtgga acggcaccag cgacagcttg aacgcaaggc
     2401 cggcgatgag cagcgcgccg ccggtgagca tgtacaggtc gcgcgtcacg tgcaggccgt
     2461 tcatgcgcgg cgccagctcg gcgaaggcca gcgtgccggt gcgcgcgtag atcagcgcca
     2521 tgccgaacag caggaaggcc gacgccgcgg ccgacagcac caggtatttg acgccggctt
     2581 ccagcggatg acggttcttc agcgggtacg ccaccatgcc gtagagcggg atcgacagca
     2641 gttccagccc gaggaacagc gaggcgaaat gcttgctgca ggcgagcagg atggcaccga
     2701 gcgacgacag catcagcagc aggtagatct cttccttgtt gccggcgtag ccgtcgatgt
     2761 acgcgtgcga cagcgtggcg gtggccagcg tggccgacag gatcaggccc atatagaaga
     2821 tgctgtacga gtcgatcacc accagcggcg tgatgctctg cggcaggtac tgcggcacca
     2881 cgccggggaa catcagcggg ccgaagaaca gcaccgagca ggccagcagc gcggcgttga
     2941 gcccggccac ggcgaaagtc gcgttccacc agtgatggcg cttgatggca atggccatca
     3001 tcaccacgac aatggtcagg ctgatgatca ggatcggcga cagcgccagc cactgggcga
     3061 aggtcatctt catggcgcca ccgccgcgtg tgccgccgcg tagacctgct gcaccgcctg
     3121 catcgaggcg gccgaggtgt tgagcacggt ctgcggatac aggcccagcc acagcaggat
     3181 ggccatcagg ctcatcatca gcaccagctc gcgcttggac aggtcttcca ggtgatcgcc
     3241 cagcgactgg ttgggcgcgc cgtggaaggc acgctgcacc atcatcagcg agtagaccgc
     3301 agccaggatc aggccgctgg cggcgaagat ggttaccgtg tggttcaccg gccaggtacc
     3361 gagcaggatg aggaactcgc cgacgaagtt gcccaggccc ggcaggccca gcgaagcggc
     3421 ggcgaagaac agcgcgatcg gcggcaggta cgggaagttg gcccacagcc cgcccatctg
     3481 cgtgaggtcg cgcgtgtgca ggcgctcgta gatttcaccg cacaggatga acagcgcgcc
     3541 ggccgaaatg ccgtgcgcca gcatctgcat gaccacgccc tgcagcgcct gcggcgtgcc
     3601 ggcgtagatg ccgaccagca cgaagcccat gtgcgaaatc gacgagaacg ccaccaggcg
     3661 cttgatgtcg gtctgtgcga aggtcatcac accgccgtag atgatgccag cgatgcccag
     3721 ccacatggcc accggcgcga agctggcgga agccgcggga aacaggggaa tgccgaagcg
     3781 gatcatgccg aaggcagccg tcttcagcag gatgccggcg aggtccaccg agccggccgt
     3841 cggcgcctgc gagtgcgcgt ccggcagcca gctgtgcagc ggcaccgccg gcgtcttcac
     3901 cgcgaaggcg acgaagaagc cgagcatcag ccagaacgcg gtggtggtcg acagcttggc
     3961 cttgagcagg tcttcgtaat cgaaggtcag cgtgccggtg gcgtggtagt taatgaacac
     4021 caggcccagg atcgacagca gcatcagcag gccgctggcc tgggtgaaga tgaagaactt
     4081 ggtcgctgcg tagatggtgc ccttgctgcc cggcgaggcg tgcccccaca gcgcgatgag
     4141 gaaatacatc ggcaccagca tcatttccca caggaagaag aacaggaaca ggtcgagcgt
     4201 gaggaacacg ccgaccacgc cgcccaggtt ccacagcagg ttgagatgga agaagccgac
     4261 gttgcgctgg atctcgcgcc aggagcaggc caccgccgcc acgccgagca ggttggtcag
     4321 cgtgaccagc agcagggcga aaccatccat ggcgaagtgg aacgagatgc ccagccgcgg
     4381 tatccactcg ccgcggtatt cgatggtcca ttgcgggtgc gcggtgccga gcccggccag
     4441 cgaccagtcg caggtgaccc acaggtagat cgagatgcct aacgagatca gcatgccgcc
     4501 cagcgcgatc cagcgcggga actgcacgcc catggtgcgc tcgccgagcc agcaggccag
     4561 gccggcgacg aagggaatca ggatgagtgc gatgaggatc atgggttcag gtccacagca
     4621 ccacggccag aaccgcggcg gcgcccgcgc ccatcgccgc cgcgtaccag cgcagcttgc
     4681 cgctctcggt gcgcgcgaag cggccgtgca gccaggtcag gaacaccggg atctggccga
     4741 tggcgacgtc gatggcgtcg ttgcggttga cgcgcacgaa ccacaggaac ggcgccttga
     4801 acacgatgtc gtacaggcgg tcgaagccga aggcggcacg cgcccagtgg gtgaacggct
     4861 cgacgatctc attgtgcgtg attgccttga gacggcgcgg gtccttgatg aacaggacat
     4921 acgccgtaag cacgcccagg ccggagacga tcaccgacag ggccgcgagc ttttccttgg
     4981 catgctcggc cgcttcgctg atcggaagtg gcggcagcac cgattcgaga tgcagcggga
     5041 tgaaaccgcc ggcaaccgcg aagaatgcca gcacacccag cggcagagag tgatcgaatc
     5101 catgcggctg gtgcggcgcg tgatcgccat gcgcgtgcga ttcgccgaag aaagcgatga
     5161 ataccaggcg gaaggtatac accgcggtga ggtaggcgcc gaaaaggccc agcgcccaca
     5221 gctccaggtg tccgctggcg taggcctcga acaggatctc gtccttgctg aagaagccgg
     5281 agaacggcgg gcacgcgatc aggccgagac agccgatcag gaacacggtg taggtgaatg
     5341 gcaactgctt gcgcagaccg cccatcttga agatgtcctg ctcgtggtgc atggcaagga
     5401 tgaccgagcc ggccgagaga aacagcagcg ccttgaagaa ggcgtgcgtc atcaggtgga
     5461 agattgccgg ctggtaggca cccacgccca ggcccaggaa catgtagccg atctggctca
     5521 tggtcgagta cgccagcacg cgcttgatgt cggtctgcgt cagcgcggtg aagctggcca
     5581 gcagcgcggt gagcgcgccg acccagccca cgcactccag cgcaaatgga gagagctcga
     5641 agatcacgtg cgtgcgcgcg atcaggtaca cgccggcggt gaccatggtg gcggcgtgga
     5701 tcagcgcgga caccggcgtg gggccggcca tggcgtccgg cagccaggtc tgcagcggca
     5761 gctgcgcact cttgcccacc gcgccgccca gcagcagcag tgcggcgagc gtggcaacgt
     5821 cgctgcccac accccagtgc tgcgcggcgc gcgccatcgc ttcgtcgatg ttgagcgtcc
     5881 cgagctggtg gaagatcagg aacaagccaa cggccatgaa ggtgtcgccc acgcgcgtga
     5941 ccacgaaggc cttgcgtgcg gcggcgccgt tggccgggtc ctggtaccag aagccgatca
     6001 gcaggtacga cgacaggccc acgccttccc agccgaagta cagaaataag agattgtcgc
     6061 ccagcaccaa aaacaacatc gaagcaacaa acagattcat ataagagaag aatctgtcgt
     6121 acgacgcgtc gccgcgcatg taccaggagg cgaacaggtg gatgaagaag ccgacgccgg
     6181 tgatgacgaa catcatggtc agcgacagcg catccagccg cagcgcgaac tgcggggcga
     6241 agcccggcac gctcatccag gtccacagca gctgcgtgta ggcggcgggc tccggctgat
     6301 gcaggaactg ccatcccgcg atgatcgtgg tcaggaacga cagcccgatg ctgcccacgc
     6361 ccaccagcgc cgcggcgttc tccggcatgc ggctgcgggc gaaggccagc gtgacgaagc
     6421 ccagcaacgg aaagacgaag acgaggaaga gaagactcat gcgatcaacc cttcatctcg
     6481 gatgccgcgt cgatgtccag cgtctggaag cggcggtaca gctgcagcag cagcgccagg
     6541 cccacgctgg cttccgacgc cgccagcgtc aggatcagga tgaacatgac ctggccatca
     6601 gggttgcccc agcgcgcgcc ggcggtgacg aaggccagtg cggcggcgtt catcatgatc
     6661 tcgatcgaca tcagcatgta gaggatgttg cggcggacca gcagcccgca gaagccgagc
     6721 gcgaacagca ccgcggccag cagcagtccg tgctccatgg gaacggcaag ggtattagtc
     6781 atgcttgccc aggtggtagg cggcgaccag cgcgccgagc agcagcatcg aaccgaactc
     6841 gaccgcgagc aggtaagggc cgtacagggc gatgcccaca cgcggcgcgc ccacttcctg
     6901 caggccggcc atgtgcaggc cgttgctcgg cagcatgtac agcagttccg ccagaagcac
     6961 ggcggacagg atcatcgggc cgatccaggc gcgcaggttc atccagcggc gttcctgctc
     7021 ggtcaccttg gagcccaggt tgagcatcat caccacgaag atgaacagca ccatgatggc
     7081 gccggcatag acgatcactt cgagcaggcc ggcgaacggt gcgccaaggg tgaagaagat
     7141 catcgccacc gacagcagcg acagcaccag gtagagcagc gcgtgcaccg gattggccga
     7201 gctgatcacc atcaccgtcg aaaacacggc gatgaaggcg gcccagtaga acggattgaa
     7261 gaaatacgcc aggaattgtt ccatcacggc atcagtccct tcacgtccac cggcggcact
     7321 tcgttggccg ccgcgccctt gggcttgccg gcgatggcca tgccggcctg ccggtagaag
     7381 ttgtactcgt gatacttgcc ggtgccggcg atctgcaggt cttccttctc gtagacgaga
     7441 ttctggcggt tgtactcgca catctcgtag tccggcgtca gctggatggc attggtcgga
     7501 caggcttctt cgcacaggcc gcagaagatg cagcgcgaga aattgatgcg gaagaattcc
     7561 gggtaccagc ggccgtcggg cttctcggcc ttctgcagcg agatgcacgc caccgggcag
     7621 gccaccgcgc acaggttgca cgccacgcag cgctcttcgc cgtcagggtc gcgcgtcagc
     7681 acgatgcgcc cgcggtagcg cggcggcacg tacggcttca cctccgggta ctggatggtg
     7741 tcgcgcttgc gccagctgtg gctgaagatg atgccgatcg agcggaactg gctccagatg
     7801 ccggcgagcg aggatttcag tgacatggtc atgcgtgccc cttcgccagc atccacgcgg
     7861 cagtgcccag cgtgttaatc agcgccagcg gcaggcagat cttccagccc gcatccatca
     7921 actggtcgta gcgcggacgc ggcagcgcag cgcggatcag gatgaagctc gacatgaaaa
     7981 tgagattctt gaaagcgaac cagaagaagc cgagccaggc ccagcgctcg acgcccgggc
     8041 caagccagcc gccgaaaaac agcgagacca tcaggcccga caccagcacg atgcccacgt
     8101 actcgccgac gaagaacatg ccgaatttca tgccggaata ttcggtatgg aagccggccg
     8161 ccagttcctg ctcggcttcc ggcaggtcga atggcgtgcg gtgggtggcg gcaacgccgg
     8221 ccagcgcgaa caggcacatg cccagcggct ggcgccacca cagccagccc tcgcgcgcct
     8281 ggtcgagcac gatctcgcgc aggttgaacg agccggccag catgaccaca cccagcagcg
     8341 acaggcccat gaatacttca taagtgatcg acagggccgt cacgcgcacg ccgccgatca
     8401 gcgagtactt gctgttcgac gaccagccgg cgagcagcac cgcgtatacc gcgaggccgg
     8461 caatggcaaa gaaatacagg atgccgagat tgaggtcggc cacccacagc gtgttcgaca
     8521 gcggcaccag ggcgaaggca atcagcatca tgctcat
//
LOCUS       DASZZC010000366         8656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1186773,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000366 DASZZC010000000
VERSION     DASZZC010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186773"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /gene="recN"
                     /locus_tag="VHE37_07835"
     CDS             <1..1691
                     /gene="recN"
                     /locus_tag="VHE37_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HVT35477.1"
                     /translation="PGSMLKHLHIRDLAIIDSVELDFAAGFTVLTGETGAGKSILIDA
                     IGLVIGTRADAALVRSGKEKAEISAEFSLRGSPAARAWLEERELTAEDDADLCLIRRV
                     VHAEGRTRAFVNGSAVNAADLRALGELLIEIFGQNESQQLLRREAQRAVLDDYGSYAP
                     ALAAVAETARAHAAVMRAIERVRSGGARDPAQVDYLRHRVRELEALALREGELEELEA
                     EHKRLANAGRLLDEGNAAQELLYGGENSIYDQLSQVDHLLGGLAALDQQFADGLAMTR
                     SAQAQAREAADALRRVLDRLDLDPAQLQQCERRLADIHELARKHRLKAAGLPALLQQM
                     QNELAELEQAAGSLDELERKREAALADYRRAALVLGTERRKAAKKFSAEVTALVRQLG
                     MGSAVFELAVDTSPDDPRECGDESVRFDFSANPGQAPRALARVASGGELSRISLAIQV
                     AGLSRGGAATMIFDEVDAGVGGGVAEIVGQKLCELGARRQVLCVTHLAQVAAQGQQQF
                     AISKDVRGGQTYTRVTALDAKARAEELARMLGGVEITASSQAHARELLKRAAKA"
     gene            complement(1812..2111)
                     /locus_tag="VHE37_07840"
     CDS             complement(1812..2111)
                     /locus_tag="VHE37_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35478.1"
                     /translation="MKTYWMLAALFVLPLAASADDVSQPAAQSGQEFSQAGKDIGHGN
                     IGDSFKQIGHGFRDGAKTTGHAIRKGAKATGHAIHNAVEGSSSAPGTGSTSSSGK"
     gene            complement(2108..2566)
                     /gene="fur"
                     /locus_tag="VHE37_07845"
     CDS             complement(2108..2566)
                     /gene="fur"
                     /locus_tag="VHE37_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HVT35479.1"
                     /translation="MESSDLRNVGLKVTLPRLKILEMLERSPARHMSAEDIYRQLMGS
                     DDDIGLATVYRVLTQFEAAGLVKRHHFEGGHAVFELERGHHHDHLVCVQCGHVEEFED
                     ATIEKRQREKARELGYKLHEHSLIMYGECQRENCPRLKGLTPRKPTGDLR"
     gene            2799..3275
                     /locus_tag="VHE37_07850"
     CDS             2799..3275
                     /locus_tag="VHE37_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HVT35480.1"
                     /translation="MRVALLLLSALALCGCSLIYKLPTRQGNVIEQKQLDQLQVGMSK
                     DQVRYLLGTPIAASPFRTERWDYLGYYKSPRGEAASRLVSIYFDAGKLSRMEGVQVAD
                     NAAIVNPDAKDVVERKKKEKVEDSRAKGDEDLHNQLQLPLPSTQPNSPDADKLPNP"
     gene            complement(3272..3571)
                     /locus_tag="VHE37_07855"
     CDS             complement(3272..3571)
                     /locus_tag="VHE37_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HVT35481.1"
                     /translation="MESVRLRVEVCCALPRRQFLVGLQVPQGTTLQQAVELSGVRDEF
                     PQLDWNALSLGVHGRARRPEEALREGDRVEIYRPLTADPKTARRERAAGARRKPG"
     gene            complement(3559..4944)
                     /locus_tag="VHE37_07860"
     CDS             complement(3559..4944)
                     /locus_tag="VHE37_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HVT35482.1"
                     /translation="MNIRESSYGYWSSNASFLWVAGGAVIGIGNIARLPYLMGEHGGI
                     IFLFVYLLALVLVGLPLLLTEWLIGRWMRDDVVSGFARITSAAKANPAWASLGWISFA
                     GAVLILSYYSVIAGWSAAYAFRAAMGLIHAIDAERARHVFAALAQDAERGLSWHTLFM
                     VMATIVVSHGIRDGMERASRVWVPIAFLMAITICVYAAINGNSSAALIYLLTPDFGRC
                     GWRGVLEALHLAFFTLGLGMGVMLTLGTYLPANAPLVRLAVGVVLMDTVFSLVAGFAV
                     FALIFDAGLDPQPGIAILFKVFPQALPNSGWGMLMAVLFFAMLFIITLSAGTTLLETA
                     TRFVMERQRTTRAFASTTGSILIWFVGLWSLLSFSVLSPMRFYGWTFFEWAQWLSTSL
                     LAPLAGLMICVFAGRFLPPELARGVWGERYAAGFRIWSWLLRYPARIGLIAVLLYSMG
                     VLDWLAQLWSP"
     gene            5016..6551
                     /locus_tag="VHE37_07865"
     CDS             5016..6551
                     /locus_tag="VHE37_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35483.1"
                     /translation="MAPEQVTAARRTSREDKPVPLLDGEERFRLLVEVVEDYAIFMLD
                     PDGNVVSWNAGAQRIKGYAPQEIIGRNFSVFYPSDKLASGWPQEELHRAAAAGRFEDE
                     GWRVRKDGSMFWANVVITAMHDRSGVLRGYAKVTRDMTQRKKLEELESSSRRMNEFLA
                     VLAHELRNPLAPIRNAISIMQLEPVVSPTLKNCRAIIDRQLSQLTRLVDDLLDVSRIV
                     SGKIALQQAPMDLHEAIERAVEASRSQLDARQQKLELNLPTAAVMVNGDLTRLTQVLQ
                     NLLNNAVKFTPVGGRIAISMHVDRETAVVQVRDSGCGMSEQTLNNVFDLFVQGPSGQA
                     PHEAGLGIGLTIARSIAQMHGGAVRAHSPGPGKGSTFSVSLPRLQQPRDSAQAAPDHG
                     LRVLVADDNRDSADSMAMLVSLFGHQPRTVYDGPAAVAAARDFKPDLILLDLAMPGLS
                     GFEVLERVRRDEATRGSVVAAMTGYGGEQDRARTQAAGFDDHLVKPVAAADFARLLQR
                     VPR"
     gene            6574..7050
                     /gene="smpB"
                     /locus_tag="VHE37_07870"
     CDS             6574..7050
                     /gene="smpB"
                     /locus_tag="VHE37_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HVT35484.1"
                     /translation="MSKAKDEKEESPRLIAQNRRARHDYFIEETFEAGLALQGWEVKS
                     LRDGRANVAEAYVVLKNNEAFLIGAHFTPLKTASTHVQPDPTRTRKLLLHEREIAQLI
                     GKVERAGYTVVPLDLHWTRGRAKASIGLAKGKKQHDKRADLKQRDWQRQKERLLRH"
     gene            complement(7075..8409)
                     /locus_tag="VHE37_07875"
     CDS             complement(7075..8409)
                     /locus_tag="VHE37_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVT35485.1"
                     /translation="MNFSRRRFVKGLTSGALLSTLPLLPRLAQAQSSYKALVCVFLFG
                     GNDGNNTIVPLGGADYNRYASARRRIAIPAADLLPLTPAAGSASYGLHPDLAPLMPIW
                     QAGKLAPLFNVGTLVEPVTLAQVQNGTATLPSSLFSHSDQQGEWQSAYAQGASRTGWG
                     GRMADALGAINSSTSLPTVISAADLTPFTLGTTTAPLAIPSSGSFGRTISLPYAAQIS
                     QAYDSLLGSPAPNLAVRGAQNVIGAAINNSRLLGSILSGPTSIQGLFNGLNTYLASQL
                     LQVAKIIENRDFLGMQRQIFFVGTGSFDTHSDQLPQQSALFREVGSALKAFYDAMAQL
                     GVSDGVTAFTMSDFGRTLQSDSNGGTDHAWGSHHLVLGGAVNGRTTYGRFPDLTLGGP
                     DDVGEGRWLPTTSVDQYGATLASWFDVPGAALSTVFPNLGNFDTPNLGFMGA"
     gene            complement(8411..>8656)
                     /locus_tag="VHE37_07880"
     CDS             complement(8411..>8656)
                     /locus_tag="VHE37_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35486.1"
                     /translation="DDPVTASTGTSVDLGAWIAAAADTTRLINKIDNLFFHGQMPATL
                     HDALQQLIDGIPVSDRDARASAALYLALTSPDYQVEQ"
BASE COUNT         1561 a         2915 c         2919 g         1261 t
ORIGIN      
        1 tacccgggtc aatgctcaaa catctccaca tccgcgacct ggcgatcatc gacagtgtcg
       61 aactggattt cgccgccggc ttcaccgtgc tcaccggcga gaccggcgcg ggcaagtcga
      121 tcctgatcga cgccatcggc ctggtcatcg gcacgcgcgc cgacgcggcg ctggtgcgca
      181 gcggcaagga gaaggcggag atcagcgccg aattctcgct gcgcggttcg cccgccgcgc
      241 gcgcctggct cgaagagcgc gaactgaccg cggaagacga cgccgacctg tgcctgatac
      301 gccgcgtggt ccacgccgag ggccgcacgc gcgccttcgt caacggcagc gcggtcaatg
      361 ccgcggacct gcgcgcgctg ggcgagttgc tgatcgagat cttcggccag aacgagagcc
      421 agcagctgct gcgcagagag gcacagcgcg cggtgctcga cgactacggc agctacgcgc
      481 cagcgctcgc tgccgtcgcc gaaaccgcgc gcgcgcacgc tgcggtgatg cgcgccatcg
      541 agcgcgtgcg cagcggcggc gcgcgcgatc cggcgcaggt cgattacctg cgccaccgcg
      601 tgcgcgagct cgaagcgctg gccttgcgcg aaggcgaact ggaagaactc gaagccgagc
      661 acaagcgcct ggccaacgcc gggcgcctgc tcgacgaagg caatgccgcg caggaactgc
      721 tttacggcgg cgagaacagc atctacgacc agctcagcca ggtggaccac ctgctcggcg
      781 gactggcggc gctggatcag cagtttgccg acggactcgc catgacgcgc tcggcgcagg
      841 cgcaggcgcg cgaggcggcc gacgcgctgc gccgcgtgct cgaccggctc gacctcgatc
      901 ctgcgcagct gcagcagtgc gagcgccggc tggccgatat ccacgaactg gcgcgcaagc
      961 accggctgaa agcggccgga ttgcccgcgc tgctgcagca gatgcagaac gaactcgccg
     1021 aactggaaca ggcggccggc agcctcgacg aactcgagcg caagcgcgaa gcggcgctgg
     1081 cggattatcg ccgcgcggca ctggtgctgg gcaccgaacg acgcaaggcg gcgaagaagt
     1141 tttccgccga ggtgaccgcg ctggtgcgcc agctgggcat gggcagcgcg gtgttcgagc
     1201 tcgccgtcga tacttcgccg gacgatccgc gtgaatgcgg cgacgaatcg gtgcgcttcg
     1261 acttttccgc caaccccggg caggcgccac gcgcgctggc cagggtggcc agcggcggcg
     1321 agctgtcgcg catctcgctg gccatccagg tggccggcct gtcgcgcggc ggcgcggcga
     1381 ccatgatctt cgacgaagtc gatgccggcg tcggcggcgg cgtggcggag atcgtcggcc
     1441 agaagctgtg cgaactcggc gcgcgccggc aggtgctgtg cgtgacgcac ctggcgcagg
     1501 tggcggcaca gggccagcag cagttcgcca tcagcaagga cgtgcgcggc ggccagacct
     1561 acacgcgcgt gaccgcgcta gatgcgaagg cgcgcgcgga ggaactggcg cgcatgctcg
     1621 gcggcgtgga gatcaccgcg tcgagccagg cgcacgcgcg cgaactgctc aagcgcgccg
     1681 ccaaggcctg aaaccgcttc aggagagctt caccgcacct gtgctggtcg tgttattaat
     1741 tgttgtctta ttataaaaac agacaatttt ttataacacg ctggatggcc gtgcggtcga
     1801 cggatttttc gtcacttgcc gctgctgctg gtgctgcccg tgcccggcgc gctgctgctg
     1861 ccctcgaccg cattgtggat ggcgtggccc gtggcctttg cgcccttgcg gatcgcatgt
     1921 cccgtggtct tcgcaccgtc gcggaagccg tggccgatct gcttgaagct gtcgccgatg
     1981 ttgccgtggc cgatgtcctt gcccgcttgg ctgaactcct gccctgactg cgccgcgggc
     2041 tgggacacgt cgtcggcact tgccgccagt ggaagcacga acagcgcagc cagcatccag
     2101 taagtcttca tcgcaggtct ccggtgggtt tgcgcggagt cagccccttg agccgcgggc
     2161 agttctcgcg ctggcattcg ccatacatga tcagactgtg ttcgtgcagc ttgtatccca
     2221 gttcgcgcgc cttttcacgc tggcgcttct cgatggtggc gtcctcgaac tcctccacgt
     2281 ggccgcactg cacgcacacc aggtgatcgt ggtggtggcc gcgctccagc tcgaacaccg
     2341 cgtgcccgcc ttcgaagtga tgccgcttga ccaggcccgc cgcctcgaac tgcgtgagca
     2401 cgcggtacac ggtggccagg ccgatgtcgt cgtcggaacc catcagctgg cggtagatgt
     2461 cttccgccga catgtggcgc gcggggctgc gctccagcat ttccaggatc ttcaggcgcg
     2521 gcagcgtgac cttcagcccc acgttgcgca gatcggatga ttccacggct ctcctcttta
     2581 aagctatttt caaattgtag cgagcgaagg cagtttgcac ggcgccggaa ctcagggtcg
     2641 gagcatgtct ccgacgagca tatgcggaaa acgcgagcat tccgagcacc gccgacatgc
     2701 aacatgccga gcgcagtaaa tctggaaaca gcttcacggc gtcgttgcgg tttccagtat
     2761 tatcgcaccc catcgcaaca ccgtccgccg aatttcccat gcgcgtcgcc ctgctcctgc
     2821 tgtccgccct cgccctttgc ggctgctcgc tgatctacaa gctgccgacg cgccagggca
     2881 acgtcatcga gcagaagcag ctggatcagc tgcaggtcgg catgagcaag gaccaggtgc
     2941 gctacctgct gggcacgccg atcgcggcca gcccgttccg cacggagcgc tgggactacc
     3001 tgggatatta caagtcgccg cgcggcgaag ccgccagccg cctggtcagc atctacttcg
     3061 acgccggcaa gctgagccgg atggaaggcg tgcaggtggc cgacaacgcg gccatcgtca
     3121 accccgacgc caaggacgtg gtcgagcgca agaagaaaga gaaagtggaa gacagccgcg
     3181 ccaagggcga cgaggacctg cacaaccagc tgcagctgcc gctgccgagc acgcagccaa
     3241 actcgcccga cgccgacaag ctgcccaacc cctagcccgg cttgcgccgc gcgccggcgg
     3301 cacgctcgcg ccgcgcggtc ttggggtcgg ccgtcagcgg ccggtagatt tccacgcggt
     3361 cgccctcgcg cagcgcttcc tccggccgcc ttgcccgccc atgcacgccg aggctgagcg
     3421 cgttccagtc cagctgcgga aattcatcgc gcacgcccga gagttccacc gcctgctgca
     3481 gcgtggtgcc ttgcggcacc tgcagaccaa cgaggaactg acgccgcggc agggcgcagc
     3541 acacttccac gcgcagcctc acggactcca cagctgcgcc agccagtcga gcacgcccat
     3601 cgagtacagc agcaccgcga tcagcccgat gcgcgccggg tagcgcagca gccacgacca
     3661 gatcctgaag ccggcggcgt agcgctcgcc ccacacgccg cgcgccagtt ccggcggcag
     3721 gaagcggccg gcgaagacgc agatcatcag cccggccagc ggcgccagca ggctggtgga
     3781 cagccactgc gcccactcga agaaggtcca gccgtagaag cgcatcggcg acagcaccga
     3841 gaacgacagc agcgaccaca ggccgacgaa ccagatcagg atgctgcccg tggtggaggc
     3901 aaacgcacgc gtggtgcgct ggcgctccat gacaaaacgc gtcgccgtct ccagcagcgt
     3961 ggtgccggcc gacagcgtga tgatgaacag catggcgaag aacagcaccg ccatcagcat
     4021 gccccagccg ctgttgggca gcgcctgcgg aaacaccttg aacaggatgg cgatgcccgg
     4081 ctgcggatcg agcccggcgt cgaagatcag cgcgaacacg gcgaagccgg ccaccagcga
     4141 gaacaccgta tccatcagca ccacgcccac cgcgaggcgc accagcggcg cgttggccgg
     4201 caggtaggtg cccagcgtca gcatcacgcc catgcccagc ccgagcgtga agaacgccag
     4261 gtgcagcgcc tccagcacgc cgcgccagcc gcagcggccg aagtccggcg tcagcaggta
     4321 gatcagcgcc gccgaggaat tgccgttgat ggcggcgtag acgcagatgg tgatggccat
     4381 gagaaacgcg atcggcaccc acacccggga ggcgcgctcc atgccgtcgc ggatgccgtg
     4441 cgacaccacg atcgtcgcca tcaccatgaa cagcgtgtgc cacgacaggc cgcgctcggc
     4501 atcctgcgcc agcgcggcga acacgtggcg cgcgcgctcg gcgtcgatgg catggatcag
     4561 ccccatcgcc gcgcggaagg cataggccgc gctccagccg gcgatgacgc tgtaatagga
     4621 caggatcagc accgcgccag cgaagctgat ccagccgagc gaggcccagg ccggattggc
     4681 cttggccgcg ctggtgatgc gggcaaagcc gctgaccacg tcatcgcgca tccagcggcc
     4741 gatcagccat tcggtcagca gcagcggcag cccgaccagc accagcgcca gcaggtagac
     4801 gaacaggaag atgatgccgc cgtgctcgcc catcaggtac ggcaggcgcg cgatgttgcc
     4861 gatgccgatc accgcgccgc cggccaccca caggaacgag gcgttgctgc tccagtagcc
     4921 atagctgctc tcgcggatat tcatgggagc tagtttatcc atgcggcacg cgggcgggct
     4981 ctgagtcata atcgccccgg caacgaggac caaggatggc tcctgaacag gttacagccg
     5041 cgcgcaggac gtcgcgcgaa gacaagccgg taccgctact ggacggcgaa gaacgcttcc
     5101 gcctgctggt cgaggtggtg gaggactacg ccatcttcat gctcgacccc gatggcaacg
     5161 tggtcagctg gaacgccggc gcgcaacgca tcaagggcta cgcgccgcag gaaatcattg
     5221 gccgcaattt ctcggtgttc tacccgtcgg acaagctcgc ctcgggttgg ccgcaggagg
     5281 aactgcaccg cgctgccgcc gccggacgct tcgaggacga aggctggcgc gtacgcaagg
     5341 acggcagcat gttctgggcc aacgtggtca tcaccgccat gcacgaccgc agcggcgtgc
     5401 tgcgcggcta cgccaaggtc acgcgcgaca tgacccagcg caagaagctc gaagagctgg
     5461 aaagctcgag ccggcgcatg aatgaattcc tcgccgtgct ggcgcacgaa ctgcgcaacc
     5521 cgctggcgcc gatccgcaac gccatcagca tcatgcagct ggagccggtg gtcagcccga
     5581 cgctgaagaa ctgccgggcc atcatcgacc gccagctgtc acagctgacc cggctggtcg
     5641 atgacctgct cgacgtcagc cgcatcgtct ccggcaagat cgcgctacag caggcgccga
     5701 tggacctgca tgaggccatc gagcgcgcgg tggaagccag ccgcagccag ctcgacgcgc
     5761 ggcaacagaa actggaactg aacctgccga ccgcagccgt catggtcaat ggtgatctca
     5821 cgcgcctgac ccaggtgctg cagaacctgc tcaacaacgc ggtcaaattc acgccggtag
     5881 gcgggcgcat cgccatctcg atgcacgtcg accgggaaac cgccgtggtg caggtgcgcg
     5941 acagcggttg cggcatgagc gagcagacgc tgaacaacgt gttcgatctc ttcgtgcagg
     6001 gccccagcgg acaggcgccg cacgaggccg gcctgggcat cgggctgacc atcgcccgct
     6061 ccatcgcgca gatgcacggc ggcgccgtgc gcgcccacag ccccggccct ggcaagggca
     6121 gcactttctc ggtgagcctg ccgcggctgc agcagccgcg cgactccgcg caagccgcgc
     6181 cggaccatgg actgcgtgtg ctggtggccg acgacaaccg cgactccgca gacagcatgg
     6241 ccatgctggt aagcctgttt gggcaccagc cgcgcaccgt ctacgacggc ccggccgcgg
     6301 tggcggcggc gcgcgacttc aagcccgacc tgatcctgct cgacctcgcc atgcccgggc
     6361 tgtccggctt cgaggtgctc gagcgcgtgc gccgggacga agccacgcgc ggcagcgtgg
     6421 tggcggccat gaccggctat ggcggcgaac aggaccgcgc gcgtacgcaa gcggcggggt
     6481 tcgacgacca cctggtcaag ccggtggcgg ccgccgactt cgcgcggctg ctgcagcggg
     6541 tgccgcgctg aggcaggttt tataatcgcg gggatgagca aggcaaaaga cgaaaaagaa
     6601 gaaagcccgc ggctcatcgc ccagaaccgt cgcgcgcgcc acgactactt catcgaggaa
     6661 acctttgagg ccggcctcgc gctgcagggc tgggaggtga agagcctgcg cgacggccgc
     6721 gccaacgtgg ccgaggccta cgtggtgctg aagaacaacg aggcgtttct catcggcgcg
     6781 catttcacgc cgctgaagac cgcctcgacc cacgtgcaac ccgaccccac gcgcacacgc
     6841 aagctgctgt tgcatgagcg cgagatcgcg cagctgatcg gcaaggtcga gcgcgccggc
     6901 tacaccgtgg tgccgttgga cctgcactgg acgcgcggcc gcgccaaggc gtcgatcggg
     6961 ctggccaagg gcaagaagca gcacgacaag cgtgcggacc tcaagcagcg cgactggcag
     7021 cggcaaaagg aacgcctgct gcgtcattga gccgcgggtt tgcgccggct tcgatcaggc
     7081 gcccatgaat cccaggttgg gcgtgtcgaa attgccgagg ttgggaaaca cggtcgacag
     7141 cgctgcgccc gggacatcga accacgacgc cagcgttgca ccgtactggt cgaccgaggt
     7201 ggtcggcaac cagcgtcctt cgccgacgtc gtcggggccg cccagcgtga gatcgggaaa
     7261 ccggccgtag gtggtgcgtc cgttcacggc gccgcccagc accaggtggt gactgcccca
     7321 agcatggtcg gtgcctccgt tggaatcgga ctgcagcgtg cggccgaagt cggacatggt
     7381 gaaagccgtc accccgtcgc tgacgcccag ttgcgccatg gcgtcgtaga aagccttgag
     7441 cgcgctgccc acttcgcgga acagggccga ttgctgcggc agctggtcgg aatgggtatc
     7501 gaagctgccg gtgccgacga agaagatctg ccgctgcatg ccgaggaaat cacggttctc
     7561 gatgatcttc gccacctgca gcagctgcga tgccaggtag gtgttcaggc cgttgaacag
     7621 cccctggatc gaggtgggcc ccgacaggat ggatccaagc aggcggctgt tgttgatcgc
     7681 cgcgccgatc acattctgtg ccccgcgcac cgcaaggttg ggtgccggcg aacccagcag
     7741 gctgtcatag gcctggctga tctgggccgc atagggcagg ctgatggtgc ggccgaacga
     7801 tccgctggac ggaatggcca gcggcgcggt ggtcgtgccc agggtgaacg gcgtcaggtc
     7861 ggccgcggag atcaccgtcg gcagcgaggt tgagctgttg atggcgccga gggcgtcggc
     7921 catgcgcccg ccccagccgg tgcggctggc gccctgggca taggccgact gccattcccc
     7981 ctgctggtcg gaatgcgaga acagcgacga cggcaaggtg gcagtgccgt tctgcacctg
     8041 ggccagcgtc accggctcga ccagtgtgcc cacattgaac agcggcgcca gctttcccgc
     8101 ctgccagatc ggcatcagcg gcgccaggtc gggatgcagg ccatagctcg cgctgcccgc
     8161 ggcgggcgtc agcggcagca ggtcggccgc cgggatggca atgcggcgcc gcgcggacgc
     8221 gtagcggttg tagtccgcgc cgccgagcgg aacgatggtg ttgttgccgt cgttgccgcc
     8281 gaacaggaac acgcagacca gcgccttgta actggactgc gcctgggcca ggcgcggcag
     8341 cagcggcagc gtgctgagca gcgcgccgga ggtcagaccc ttcacgaacc ggcgtcggga
     8401 aaagttcatg tcactgctcc acctgatagt cgggcgaggt cagcgcgagg tacagcgcag
     8461 cgctggcgcg cgcgtcgcga tcactgaccg ggatgccgtc gatcagctgc tgcagcgcgt
     8521 cgtgcagcgt ggccggcatc tgaccatgaa aaaacagatt gtcgatcttg ttgatcagcc
     8581 gcgtggtgtc cgcagccgcg gcaatccacg cgccgaggtc caccgaggtg ccggtcgatg
     8641 ccgtcaccgg atcgtc
//
LOCUS       DASZZC010000367         7039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1055047,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000367 DASZZC010000000
VERSION     DASZZC010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055047"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..784)
                     /locus_tag="VHE37_07885"
     CDS             complement(323..784)
                     /locus_tag="VHE37_07885"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HVT35487.1"
                     /translation="MKSVRAALLLACLCAVQPDSWASCSASSQSVPFPGYNPFDLTAD
                     DATGNVHVSCTGIGLLVSYHISLSTGASGSFTTRTMKQASATLNYNLYTNLLRVTIWG
                     DGSGSSSKVDDGYLIQLGTIDRDYTVYGRVFAQQNAAPGPYSDTILVTVDY"
     gene            complement(795..3071)
                     /locus_tag="VHE37_07890"
     CDS             complement(795..3071)
                     /locus_tag="VHE37_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HVT35488.1"
                     /translation="MPLLALLTLPAHAAGNAAPEWFGAPCSSSEELPAAAGEMLLSVR
                     RQPDDPGSDELVLRTVDGRMWVQRETLRSWNLPPPPVMQDFRGAPWYALAAIDGVRYR
                     YDSCLQVLWLDAATAQPLVRYAVEVPPGPPPDTAAPGGFFNIEPQYTHASGAPAWSGL
                     AHAGLFNGWGYGGSSLLEDGSHPVRLDTNWVVDDLDHLQRLSLGDGISRSSTFGRSLH
                     FGGVQWGREFSLRPDLVTFPQPALRGDASLPSSVDVYVNQMLRSHQDVPAGPFELDRI
                     PVVTGSGDVLLVVRDLLGREQVISSSFYASPQLLTAGLTDYTFEAGFPRNSYGVRSND
                     YGALFGAGTWRRGYADWLTGEFRGELARDRQLLGASAAMLVPRVGTANIGVAASSGDA
                     GLGGSAVFGIERVSPRWSVGAEARQSTAHFRQLGGDFMAIRNSQIVHVGVVPHISGSL
                     SLAWVHQQYFDGDDTRIASLNYMLKVGRDVYANLSVTDARSASDDRSYLLSLTSSFGG
                     NVSGSVQHEFQPGREPRNRLDVQRSPSGPLGLTLQAAADDGGDYRRQFDARWADQRGT
                     TTADYAQDGGQREERIGYSTGFALLDDSFYWTRPVEGSFALVEVPSTPDVAVYANEHY
                     AGRTDGNGKLLVPDLRAYEANTLRLDDADLPMDREVTSLRSRVVPPPLSGVTYRFPLR
                     INRELLLHLKRLDGSAVPAGASIAGQDLPVGFGGVASVGGISTAQALQVQWDSGSCRA
                     WIAPPAVADAQPIDVVCR"
     gene            complement(3082..3819)
                     /locus_tag="VHE37_07895"
     CDS             complement(3082..3819)
                     /locus_tag="VHE37_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HVT35489.1"
                     /translation="MRKMLIGLLLLGAGCVCAATTPREGLTVSPVQTTLEPGQRATSV
                     TMVNSTDDNKVIQAEVMRWTHVDGQDHYEPAGELLVNPPLFKLAAGASQIVRVGFAQP
                     PAGREREVQYRLYLQEVPSQDQQAPFAGAQLKMLLRLGVPVFVAPAAIQAESIKWRAI
                     RRGSDIELQLTNASNAHVRAAEVALGPHDGKPEDFVHANSFAYVFPGETHSWTLQPNW
                     PSAQKQLTVQARTDRGETRVSIPLEAP"
     gene            complement(3863..4360)
                     /locus_tag="VHE37_07900"
     CDS             complement(3863..4360)
                     /locus_tag="VHE37_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HVT35490.1"
                     /translation="MHMRRVGIAGLMVLAAGMPPAATASQATSSFLVTATVLNVCTVT
                     ATALPFTNYDPTASGNDDATTTVSVLCTLSTPYTVRLSQGVNGTSVTARKMIRTLGTE
                     TLTYSLYRDAARSQNWGVTDGSDTASGTGTGLLQNITVYGRITSGAAVPAGAYTDTVT
                     VTVNY"
     gene            complement(4422..4910)
                     /locus_tag="VHE37_07905"
     CDS             complement(4422..4910)
                     /locus_tag="VHE37_07905"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HVT35491.1"
                     /translation="MKVGKVLTAGLLGMAPLAAMASQASGTFAVGTTVLSVCTVVASP
                     LTFINYDPTASGNTDGTTTIGVLCTNGTSYTVRLSQGANGTGVTARKMLLTGGSDLLP
                     YSLYRDSSRSQNWGVTDGSDTASGTGNGLLQNVTVYGRIGSGSTASAGIYLDTINVTV
                     NY"
     gene            5102..6415
                     /locus_tag="VHE37_07910"
     CDS             5102..6415
                     /locus_tag="VHE37_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVT35492.1"
                     /translation="MLDEFLTRLRDWLWSERGSLLAPGFARRTGRYVWAVARDLADGQ
                     INMRAMSLVYTTLLSVVPLLALSFSLLKAFGVHNMLQPVLLRVLAPLGTQAGELADQI
                     VGFVDKIKVGVLGSLGLALLFWSALSLIQKVEGSFNYIWHVKRPRPLGQRVGEYMAVL
                     LVGPVLVFSAIGLTASVFSGSIAATLAGIEPFGFLLYLGSKALPYLLIIGAFAFAYIY
                     IPNRGVSLRAAAIGGTVAGFAWQAASVAFASFVSRSTNYNAVYSGFAIVIFLLIWIYV
                     GWLILLVGCLLTYYLEHPEKLRVQDESVAAAGRQREQLALLAMVLCGRRFMRGEPGYT
                     AEQLTQALGCAPEPAGDVIDALLHQGLLAETAGEPVRLLPARDPSALPMAQVWLAARG
                     ARAALQRADADARALAELIERAEQDFARDAGAVALREWIERMHES"
     gene            6620..>7039
                     /locus_tag="VHE37_07915"
     CDS             6620..>7039
                     /locus_tag="VHE37_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35493.1"
                     /translation="MLAHQLLRGTLVAALCALSFEAAAIPEGPDYPSPAWIEREAANY
                     AKTAEGPAEEAENPAFIARWEEQQLANQQSWNERWLNDPTWLGYPSGNTTQTPLCTVW
                     FAQCSGNPFDYPDTDPFYVNEGEVTPFVIYDDGCARLS"
BASE COUNT         1202 a         2293 c         2309 g         1235 t
ORIGIN      
        1 tgggtcttga ccagcgtctg cacggtctcg accacgatcg cgttcgcgga cttcagcgtc
       61 tcaaaaccgg cgagcaccgc ttcctgaccc tgcgtgagga tttcctggcc ctgctcgatg
      121 atcggctcaa cgcggtcctt cacatcggca acaatcgatt caacattcat gcttgcgctc
      181 cattgggtga ttgaaaactg gctgcttctt catactcaat gctgcgtcgc agcatttgac
      241 ggggcgcaga ttaatgttgc gtcgcaccat tgtcaacaga aatccgtcaa gctaatgaaa
      301 ttattgtggt tatttattgt cttcaatagt cgaccgtcac caggatggta tcggaatacg
      361 gccctggagc cgcattctgc tgggcaaaca cgcggccgta gacggtgtaa tcccggtcga
      421 tcgtgcccag ctggatcaga tagccgtcat ccaccttcga cgagctgccg ctgccgtcgc
      481 cccagattgt tacccgcaac aggttggtgt acaggttgta gttgagcgtc gccgaagcct
      541 gcttcatcgt gcgcgtggtg aagctgccgg aagcgccggt cgacagcgag atgtggtaac
      601 tcaccagcaa gccgataccc gtgcagctca catgcacgtt gccggtggca tcgtcggcag
      661 tcaggtcgaa cgggttgtag ccggggaacg gcacgctctg cgacgacgcg ctgcacgaag
      721 cccacgagtc gggctgcacg gcgcagaggc aagccagcaa cagcgcggcg cgaacggatt
      781 tcatcccggt catgttagcg gcataccacg tcgatcggct gcgcatcggc gaccgctggc
      841 ggcgcaatcc atgcacggca actgccgctg tcccactgca cctgcagcgc ctgcgcagtg
      901 ctgatcccgc caacggaggc gacgccgccg aaaccgacgg gcagatcctg cccggcgatg
      961 ctcgcgcccg ccgggacggc gctgccatcc agccgcttca ggtgcagcag caactctcga
     1021 ttgatgcgca gcgggaagcg ataggtcaca ccgctcaacg gcggcggcac cacgcggctg
     1081 cgcagcgagg tcacttcgcg gtccatcgga agatcggcat cgtccagccg cagggtgttg
     1141 gcctcgtagg cgcgcagatc ggggaccagc agcttgccgt tgccgtcggt acggccggcg
     1201 taatgttcgt tggcgtacac cgccacgtcc ggcgtcgacg gcacttcgac cagtgcaaag
     1261 ctgccttcga ccgggcgcgt ccagtagaag ctgtcgtcga gcagggcgaa tccggtcgaa
     1321 tagccgatgc gctcttcgcg ctgtccgccg tcctgcgcgt aatccgcggt ggtggtcccg
     1381 cgctgatcgg cccagcgcgc gtcgaactgg cgcctgtagt caccgccgtc atccgctgcg
     1441 gcctgcaagg tcagtcccag ggggccgctc gggctgcgtt gcacgtccag ccggttgcgc
     1501 ggctcgcgcc ccggctggaa ctcatgctgg acactgccgc tgacattgcc gccgaaagaa
     1561 ctggtcagac tgagcagata gctgcggtca tcgctggcgc tgcgcgcgtc ggtcaccgac
     1621 aggttggcgt agacatcgcg cccgaccttg agcatgtaat tgaggctggc gatgcgcgtg
     1681 tcgtcaccgt cgaaatactg ctgatgcacc caggccagcg acagcgaacc gctgatatgc
     1741 ggcaccacgc cgacatgcac gatctggctg ttgcggatcg ccatgaaatc gccgccgagc
     1801 tggcggaaat gggcagtcga ttgccgcgcc tccgcgccga cgctccagcg cggcgacacg
     1861 cgctcgatgc cgaacaccgc agagccgccg aggcccgcgt cgccgctgct ggccgcaaca
     1921 ccgatattgg ccgtgcccac gcgcggcacc agcatggcag cggaggcgcc gagcaactgg
     1981 cggtcgcgcg ccagctcacc gcggaattcc ccggtcaacc agtcggcgta gccgcgtcgc
     2041 caggtaccgg caccgaacag cgcgccatag tcgttggagc gcacgccata gctgttgcgc
     2101 ggaaagccgg cctcgaaggt gtagtcggtc agccccgcgg tgagcagttg cggcgaggcg
     2161 tagaacgacg acgagatcac ctgctcgcgg cccagcaggt cgcgcaccac cagcagcacg
     2221 tcgccgctgc cggtcaccac cgggatgcgg tccagctcga acggtccggc gggcacatcc
     2281 tggtgcgaac gcagcatctg gttgacgtag acatcgaccg acgagggcaa gctggcatcg
     2341 ccgcgcagcg ccggctgcgg aaaggtgacc aggtcggggc gcagcgaaaa ttcacgaccc
     2401 cactgcaccc cgccgaaatg cagagatcgg ccgaacgtgc tcgagcggct gatgccgtca
     2461 cccagcgaca ggcgctgcag gtgatcgaga tcgtcgacga cccagttggt atccagacgc
     2521 accggatggc tgccgtcctc aagcagcgag ctgccaccat agccccagcc gttgaacagg
     2581 cccgcgtgcg caagccccga ccacgccggt gcgccggacg cgtgcgtata ttgcggttcg
     2641 atgttgaaga agccgccggg cgcggcggtg tcgggcggag ggcccggcgg cacctccacc
     2701 gcatagcgca ccagcggctg cgcggttgcg gcgtcgagcc acagcacctg caggcagctg
     2761 tcgtagcggt aacgcacgcc gtcgatggcg gcgagtgcat accagggcgc gccgcggaag
     2821 tcctgcatga ccggcggcgg cggcagattc cagctgcgca atgtttcccg ctgcacccac
     2881 atgcgtccgt cgacggtgcg cagcaccagt tcgtcgctgc ccggatcatc tggctgccgg
     2941 cgcaccgaca gcagcatttc gccggcagcc gcgggcaact cctcgctgga actgcacggt
     3001 gcgccaaacc attccggcgc tgcgttgccg gcggcatgcg ccggcagcgt cagcagcgca
     3061 agcagcggca actgaaatag gctaaggcgc ttccagcggg atgctgacac gcgtttcgcc
     3121 ccggtcggta cgcgcctgca cggtcaactg cttctgcgcc gacggccagt tcggctgcaa
     3181 ggtccagcta tgcgtttcac ccgggaacac gtaggcgaaa gagttggcat ggacgaagtc
     3241 ttcgggcttg ccatcgtgcg gacccagcgc cacctcggcg gcgcgcacgt gcgcgttgct
     3301 ggcattggtc agctgcagtt cgatgtcgct gccgcggcgg atggcacgcc acttgatcga
     3361 ttcggcctgt atcgctgccg gcgccacgaa caccggcacg cccaggcgca gcagcatctt
     3421 cagttgtgcg ccggcaaagg gcgcctgctg atcctgcgac ggcacctcct gcaggtacag
     3481 gcggtactgc acctcgcgct ccctgccggc cggcggctgg gcaaagccca cgcgcacgat
     3541 ctggctggcg ccggcagcga gcttgaacag cggaggattg accagcaact cgccggccgg
     3601 ctcgtaatga tcctggccat cgacatgagt ccagcgcatc acctcggcct ggatcacctt
     3661 gttgtcgtcg gtgctgttga ccatggtcac cgaggtcgcg cgctgccccg gctcgagcgt
     3721 ggtctgcacc gggctgacgg tgaggccctc tcgcggcgtg gtggccgcgc aaacgcagcc
     3781 ggcgcccaac agcagcaggc caatgagcat cttgcgcatg atcgtctcca ctcttccctt
     3841 tgtggaccgc cgcagcggat gcctagtaat tgaccgtgac cgttaccgta tcagtatagg
     3901 cacccgccgg caccgcagcg ccggaggtga tgcggccata cacggtgatg ttctgcagca
     3961 ggccggtgcc ggtgccgctg gcggtgtcgc tgccgtcggt cacgccccag ttctgcgagc
     4021 gcgcagcatc gcggtacaag gaataggtca gtgtttcggt gcccagcgtg cggatcatct
     4081 tgcgcgcagt caccgacgtt ccgttgacgc cctgcgacag gcgcaccgtg tacggcgtcg
     4141 acagcgtgca cagcaccgag acggtggttg tcgcatcgtc gttgcccgac gcggtgggat
     4201 cgtagttggt aaagggcagt gccgttgccg tcaccgtgca cacgttgagc acggtggcgg
     4261 tcaccaggaa cgaactggtc gcctgcgagg ccgtcgccgc tggcggcatc cctgccgcca
     4321 gcaccatcaa cccggcaatc ccgaccctgc gcatatgcat ggccccgact tccctgtttg
     4381 gctgcagacg aacttcgaaa tgaacttcga atggaccgcg atcagtaatt gacagtgacg
     4441 ttgatggtgt ccaggtagat gccggctgaa gccgtcgagc cggaaccgat gcggccgtac
     4501 accgtgacat tctgcagcaa gccgttgccg gtgccgctgg cggtgtcgct gccgtcggtc
     4561 acgccccagt tctgcgagcg cgaggaatcg cggtacagcg agtacggcaa caggtcggag
     4621 ccgccggtca gcagcatctt gcgcgcggtc acgccggtgc cgttggcgcc ctgcgacagg
     4681 cgcacggtat acgaggtgcc gttggtgcac agcacaccga tggtggtggt gccgtcagtg
     4741 ttgccactgg cggtcgggtc gtagttgatg aacgtcagcg gcgacgccac tacggtgcag
     4801 accgacaaaa ccgtggtgcc cacggcgaac gtgccgctgg cctgcgaagc catcgccgcc
     4861 aggggcgcca tgcccagcaa gcccgccgta agaactttgc caaccttcat gtcatcttct
     4921 ccagaatttg gtggaacgaa ttggtcccac ggcgcgtaag ctatcagcag aagcgcagcg
     4981 gaaaagcgtc tgtcggatat ctggcgcgcg caatggaatg agcggcgcat cagctacccg
     5041 gcggtagcaa acgcacggct ggacggcgcg gccatccaca acgaaccggt atcttttgcc
     5101 catgctcgac gaatttctga cgcgcttgcg cgactggttg tggtccgaac gcggcagcct
     5161 gctggcgccc ggcttcgcgc ggcgcaccgg ccgctacgta tgggcggtgg cgcgcgatct
     5221 ggccgacggc cagatcaaca tgcgcgcgat gagcctggtg tacaccacgc tgctctcggt
     5281 ggtgccgctg ctggcgctgt cgttctcgct gctcaaggcg ttcggcgtgc acaacatgct
     5341 gcagccggtg ctgctgcgcg tgctcgcgcc gctgggcacg caggccggcg aactggcgga
     5401 ccagatcgtc ggcttcgtcg acaagatcaa ggtgggcgtg ctcggttcgc tcggtcttgc
     5461 actgctgttc tggtcggcgc tgtcgctgat ccagaaagtg gaaggcagct tcaactacat
     5521 ctggcacgtg aagcggccgc gaccgctggg ccagcgcgtg ggtgagtaca tggcggtgct
     5581 gctggtcggt ccggtgctgg tgttctcggc gatcgggctc accgccagcg tgttctccgg
     5641 cagcatcgcc gccacgctgg ccggcatcga gcctttcggt ttcctgctgt atctcggctc
     5701 gaaggcgctg ccgtatttgc tgatcatcgg cgcgttcgcc ttcgcctaca tctacatccc
     5761 caaccgcggc gtcagcctgc gcgcggcggc catcggcggc accgtcgccg gcttcgcctg
     5821 gcaggcagca agcgtggcct tcgcctcgtt cgtctcgcgt tccaccaact acaacgccgt
     5881 ctattcagga tttgccatcg tcatcttcct gctgatctgg atctatgtcg gctggctgat
     5941 cctgctggtg ggctgcctgc tcacctatta cctggagcac ccggaaaagc tgcgcgtgca
     6001 ggacgagtcg gtggcggcgg ccgggcggca gcgcgagcag ctcgcgttgc tggcgatggt
     6061 gctgtgtggg cgccgcttca tgcgcggcga gccaggctac accgcggagc agctgacgca
     6121 ggcgctgggc tgcgcaccgg aaccggcggg cgacgtgatc gacgccctcc tgcaccaggg
     6181 cttgctggcg gaaaccgcgg gtgagccggt gcgactgctg ccggcgcgcg atccgtccgc
     6241 gctgccgatg gcgcaggtgt ggctggcggc gcgcggcgcg cgggcggcgc tgcagcgcgc
     6301 ggatgcggat gcgcgcgcac tcgctgaatt gatcgagcgc gcggaacagg atttcgcgcg
     6361 cgatgccggc gccgtggcgc tgcgcgagtg gatcgagcgc atgcacgagt cctgaccggc
     6421 atcctgatgg atcggaaaca cgtccaccgc acctgcattc cgcttgttaa taaatattgt
     6481 ttatagttta aaatattata ttttttataa catacaaaat gcctatgcgg tgagcagatt
     6541 tttgcgcttg cgccatccat gcgggcttgc ttagattgcg ggcacatcgc gccagcagag
     6601 cgcgtgggag gagatcacga tgcttgctca tcagctgttg cgcggcaccc tggttgccgc
     6661 actgtgcgcg ctgtcgttcg aggcggcggc gattcccgaa gggccggatt acccgtcgcc
     6721 cgcctggatc gagcgcgagg cggcgaacta tgccaagacc gccgaaggcc cggcggaaga
     6781 agcggagaat cctgcgttca tcgcccgctg ggaagaacag cagctggcca accagcaatc
     6841 gtggaacgag cgctggctca acgatccgac ctggctcggc tatccgtcgg gcaacaccac
     6901 gcagacgccg ctgtgcacgg tctggtttgc gcaatgcagc ggcaatccgt tcgactatcc
     6961 cgacaccgat ccgttctacg tcaacgaagg tgaagtcacg ccgttcgtga tctacgacga
     7021 cggctgcgcg cgcctgtcc
//
LOCUS       DASZZC010000368         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_607047,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000368 DASZZC010000000
VERSION     DASZZC010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607047"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="purF"
                     /locus_tag="VHE37_07920"
     CDS             <1..1188
                     /gene="purF"
                     /locus_tag="VHE37_07920"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HVT35494.1"
                     /translation="VELKQELFAEDRRHLNTESDSEVLLNIFAHELMQRGSLRLTPED
                     VFAAVSGVHKRCRGAYACTVLIAGYGMVGFRDPFGIRPAVYGRRLGPNGVDYLIASES
                     VALNALGYEMVDDIAPGEAVYITADGKLHKRQCALNPIYSPCIFEYVYLARPDSVMDD
                     VYVYKARLRMGIKLAEKILREWPQHKIDVVIPIPDTSRVAGAELAARLEVPYREGFIK
                     NRYIGRTFIMPGQAQRKKSVRQKLNPVDIEFRGKNVLLVDDSIVRGTTSKEIIQMARE
                     SGAKKVYFASAAPPVRFPNVYGIDMPTSSELVAHGRSVEELEKLLGADGLIYQDLKDL
                     EDAVRAGNPDLTRFDSSVFTGEYVTQDISADYLSQLELFRSDAAKLARQRGGATVIEL
                     QNA"
     gene            1235..>1803
                     /locus_tag="VHE37_07925"
     CDS             1235..>1803
                     /locus_tag="VHE37_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT35495.1"
                     /translation="MSNKPDNPRWGYATQGIRASEPESAYGEHSASIALTSSFTHESA
                     AQAARVFAGEEPGYIYSRFTNPTVRAFEQRLALMEGGAACVATASGMSAILSLCLALL
                     KSGDHVLASRGLFGATINLYNNILSKFGIAVSWVDMTDVAAWERTFQPNTKLLFVETP
                     SNPLAEIADICALANLAHARGAWLCVDNC"
BASE COUNT          320 a          601 c          586 g          296 t
ORIGIN      
        1 gtggagctga agcaggaact gttcgccgag gaccgccggc acctgaacac ggaaagtgat
       61 tccgaggtgc tgctgaacat cttcgcccat gagctgatgc agcgcggcag cctgcggctg
      121 acgccggaag acgtgttcgc cgcggtgtcg ggcgtgcaca agcgctgccg cggcgcctat
      181 gcctgcacgg tgctgatcgc cggctacggc atggtcggct tccgcgatcc gttcggcatc
      241 cgccccgcgg tctacggccg ccgcctgggg ccaaacggtg tggattacct gatcgcttcg
      301 gagagcgtgg cgctcaacgc gctcggctac gagatggtgg acgacatcgc acccggcgag
      361 gcggtgtaca tcaccgccga cggcaagctg cacaagcgcc agtgcgcgct caacccgatc
      421 tactcgccgt gcatcttcga gtacgtgtac ctggcgcgcc cggactcggt gatggatgac
      481 gtctacgtgt acaaggcgcg cctgcgcatg ggcatcaagc tggcggagaa gatcctgcgc
      541 gagtggccgc agcacaagat cgacgtggtc atcccgatcc cagacaccag ccgcgtggcg
      601 ggtgccgagc tggccgcgcg gctcgaggtt ccgtaccgcg aaggcttcat caagaaccgc
      661 tacatcggcc gtaccttcat catgccgggc caggcccagc gcaagaagtc ggtgcggcag
      721 aagctcaacc cggtggacat cgagttccgc ggcaagaacg tgctgctggt cgacgattcc
      781 atcgtgcgcg gcaccaccag caaggagatc atccagatgg cgcgcgagtc cggcgccaag
      841 aaggtctact ttgcctccgc cgcgccgccg gtgcgcttcc ccaatgtcta cggcatcgac
      901 atgccgacct cgtccgagct ggtggcgcac ggtcgcagcg tggaggaact cgagaagctg
      961 ctcggcgccg acggcctcat ctaccaggat ctgaaagatc tcgaagacgc ggtgcgcgcg
     1021 ggcaaccctg acctgacccg cttcgacagt tccgtgttca ccggcgagta cgtcacgcag
     1081 gacatcagcg ccgactatct gtcgcaactg gaactgttcc gttccgatgc tgccaagctg
     1141 gcgcgccagc gcggcggggc gacggtgatc gagctgcaga acgcctgagc gcggcgcgca
     1201 aatcgcgccg ggatcgcgcg tacaatcgcg cgccatgagc aacaagcctg acaacccgcg
     1261 ctggggttac gccacccagg gcatccgtgc gtctgagccg gaatccgctt acggcgagca
     1321 ttccgcgtcc atcgcgctca cctccagctt cacccacgag agtgcggcgc aggcggcgcg
     1381 cgtgtttgcc ggcgaggaac ccggctacat ctactcgcgc ttcaccaatc ccacggtgcg
     1441 cgcgttcgag cagcgcctgg cgctgatgga aggcggcgcg gcctgcgtag ccaccgccag
     1501 cggcatgagc gcgatcctgt cgctgtgcct ggcgctgctg aaatccggcg accacgtgct
     1561 ggcctcgcgt ggattgttcg gtgccaccat caacctgtac aacaacatcc tgtcgaagtt
     1621 cggcattgcg gtgagctggg tggacatgac cgacgtcgcc gcctgggaac gcacgttcca
     1681 gccgaacacg aagctgctgt tcgtggaaac accgtcgaac ccgctggcgg agatcgccga
     1741 catttgcgcg ctggccaacc tcgcccatgc gcgcggcgcc tggttgtgcg tggacaactg
     1801 ctt
//
LOCUS       DASZZC010000369         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_406308,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000369 DASZZC010000000
VERSION     DASZZC010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406308"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VHE37_07930"
     CDS             <1..439
                     /locus_tag="VHE37_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35496.1"
                     /translation="ITINHSYDQIMAWEEHAVDQQMEAGTSLNMIPGGFKGMKFLHEH
                     RLTTAPRVSIEERDNAIGAYQALNPRAGVPNLIISELWKDSRYAESVICGVEGRLSID
                     QVRKIRELFSAGVPLEKIVELSGARNTLQVERVTAQKTYTRIH"
     gene            complement(496..1533)
                     /locus_tag="VHE37_07935"
     CDS             complement(496..1533)
                     /locus_tag="VHE37_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35497.1"
                     /translation="MTVVMQPKDLNNLYEATVVFAGPSRVGTNVLDEQESNRDLLRLC
                     TVVLLTALRVSRARSRWSPLYLPVDAQRAEVAKMLEIVRAVWRARSSGVPTSIKEDDG
                     IAVVWALARSLFVESGFDWVGERALREYITVADGDHFAAARDTLVAMMVPIGVVVDGW
                     LVGGSRSQVDVARLLIAQIMDAAESIAPDIQLACSTTFEQTPAQMLAAGGNDAAWVVA
                     AIEALFFMEQELRMNANNEPVVMRRLSDACRAGGLAQRWAMVRCIGDGGGKGDLLAER
                     YDAFFGELQQLRSIKGRTGEEEALRRVWNSVRLAPADVALGQKYQAMIASCASAAIQR
                     WRMKERPAAAA"
     gene            1613..1897
                     /locus_tag="VHE37_07940"
     CDS             1613..1897
                     /locus_tag="VHE37_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVT35498.1"
                     /translation="MRKAITKSTLLEDIASGTQGIPKSTVKAVMDEFVQVTGRHLHAG
                     NKVVIKGFGTFEVRKRAGRIGRNPKTGAPLRIDPSKIVAFKANPSFRSGV"
     gene            2231..2854
                     /locus_tag="VHE37_07945"
     CDS             2231..2854
                     /locus_tag="VHE37_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35499.1"
                     /translation="MKKSKGTLAKLALAAAAMVSMMAPVSGAQAGQGAAPWTTNELVF
                     VPACKFTEGSAAVDADCAANLSALMTAAAEQGAKIAVITASNDWLSAKSLEDDQARLK
                     DLRAATMAHLLRDKNLKLNVVVFGTRAVSGQTGYVAFMKDRYEAPLGKLATFPVYQPT
                     APIPQLPLYVSAPAAATTSVAKQDASETVPVEIANQSKDRSIDVAGH"
BASE COUNT          610 a         1055 c          863 g          481 t
ORIGIN      
        1 gatcaccatc aatcacagtt acgaccagat tatggcgtgg gaagagcacg cagtagacca
       61 gcaaatggaa gccggcacct cgctgaatat gatcccgggc ggattcaagg gaatgaagtt
      121 cctccatgaa catcgcctca caacagcacc gcgcgtgtcg atcgaagaga gggataacgc
      181 catcggcgcg tatcaggcac tcaacccaag agccggtgtc ccaaacctga tcatcagcga
      241 attatggaag gacagtcggt acgctgaatc cgtgatctgt ggcgtagaag gccggctctc
      301 cattgatcag gtgcgtaaaa tcagagagct attctcggcc ggggttccac tggaaaagat
      361 cgtcgagttg tccggcgcgc gaaacaccct gcaagttgag cgcgtcaccg cccagaagac
      421 ttacacgcgg atccattaat cggcgcgggc catccgccgt ggcgccaagc agcggccaac
      481 cagcgccgcg cgctgtcaag cggccgcggc cgggcgctcc ttcatgcgcc accgctgaat
      541 ggccgccgac gcgcacgacg caatcatggc ctggtacttc tgtcccaggg ccacatcggc
      601 cggcgccagg cgcacgctgt tccatacccg gcgcagcgcc tcttcttcgc ccgtacgacc
      661 cttgattgag cgcagttgct gcaactcgcc gaaaaaggcg tcgtagcgtt ctgccagcag
      721 atcgcctttg ccgccaccgt caccgatgca acgcaccatc gcccagcgct gcgccaggcc
      781 gccagcgcgg cacgcgtccg acaagcgccg catcaccacc ggctcgttgt tggcgttcat
      841 gcgcagttcc tgctccatga aaaacagtgc ctcaatcgca gccactaccc aggcggcgtc
      901 gttgccgcca gccgccagca tctgcgccgg cgtctgttca aaagtggtgc tgcaggcgag
      961 ctggatgtct ggcgcgatcg actcggccgc gtccatgatc tgcgcgatca gcagccgcgc
     1021 cacatcaacc tgcgagcgcg aaccgccgac cagccagccg tcgacgacaa cgccgattgg
     1081 caccatcatt gccaccaggg tgtcgcgggc cgccgcgaag tgatcaccgt cagcgaccgt
     1141 gatgtactcc cgcagagcgc gctcgcccac ccagtcgaac cccgactcca cgaacagcga
     1201 acgcgccagg gcccacacca ctgcgatgcc gtcgtcttcc ttgatcgaag tcggcacacc
     1261 actgctgcgc gcgcgccaga cggcgcggac gatctccagc atcttggcca cttccgcacg
     1321 ctgcgcatcc acgggcagat acagcggcga ccagcgggac ctggcacgcg agacccgcag
     1381 cgccgtgaga agcaccactg tgcacaagcg aagcaggtcg cggttggatt cctgctcgtc
     1441 gagcacgttt gttcccacgc gcgatgggcc cgcaaacacc acggtcgcct cgtaaaggtt
     1501 gttcaaatct ttcggttgca taacgacagt catggcgtca actcttgagt gttgtgcggt
     1561 ggttcgttta caaaggcagc cgcgggccac caacggccgc acggaaactc aaatgcgcaa
     1621 ggccatcacc aagagcacgc tgctggaaga cattgccagt ggcacccagg gcattcccaa
     1681 atcgaccgtg aaggctgtga tggatgaatt cgtccaggtc accggccgac atctgcacgc
     1741 cggcaacaag gtggtcatca agggcttcgg gactttcgaa gtgcgcaagc gcgcagggcg
     1801 catcggacgc aatcccaaga ccggcgcgcc ccttcgcatc gatccgtcca agatcgtcgc
     1861 cttcaaggcc aacccgagct tccgctccgg cgtctaagcg caggggccca ctgcgggcct
     1921 cgctcatcac ctggctgttg actcctgctg gcaccggggc cgcctgcgca cgcgcgcgtt
     1981 cctatgaatg ccgcccatat tcaccccggg gcgtgcggac gcgtaccccc gaaaaacggt
     2041 atttccgccg tctaaaccgg cttctcggcc gcaaccattc ccccgaaagt aaccacaggg
     2101 cgggagcccg gaggtagcgc caaaaacggt aaaaaatccg tttttaccgc attcctcgcc
     2161 gcagcccccg ctctgaaaat ggatctacca gttgatgagc gcgcagcctg gcgccggggg
     2221 aatagttcac atgaaaaagt ccaaaggaac actcgcgaag ctggccctcg ccgccgccgc
     2281 gatggtctcc atgatggcgc ccgtgtcagg cgcccaggcc ggtcagggcg ctgcgccctg
     2341 gaccaccaat gaactggtct tcgttccggc ctgcaagttc accgaaggtt ccgccgccgt
     2401 cgacgccgac tgtgctgcca acctgagcgc gctgatgacc gcggccgccg agcagggcgc
     2461 gaagatcgcc gtcatcaccg cctccaacga ctggctcagc gccaagtcgc tcgaggacga
     2521 tcaggcccgc ctcaaggatt tgcgcgccgc caccatggcg cacctgctgc gcgacaagaa
     2581 cctcaagctc aacgtcgtgg tcttcggcac ccgcgccgtc agcggccaga ccggctacgt
     2641 cgccttcatg aaggaccgtt acgaagcgcc gctgggcaaa ctcgcgacgt tcccggtgta
     2701 ccagcccacc gcgcccatcc cgcaactgcc gctgtacgtc agcgcgcccg ccgcggccac
     2761 cacctccgtc gccaagcagg atgcttccga gacggtcccg gtcgaaatcg ccaaccagag
     2821 caaggatcgc tcaatcgacg tcgccggcca ctaatgccga tggtcgccga cctgcccccc
     2881 tgccacgtgc agatcgccga gcactacaac gtgcccgctg atctgctgca ggccattcat
     2941 gccaccgaag gcggtcgccc cggtcttgta gtcggcccga acaagaacgg cacgttcgat
     3001 tacggcgac
//
LOCUS       DASZZC010000370         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_732830,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000370 DASZZC010000000
VERSION     DASZZC010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732830"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VHE37_07950"
     CDS             complement(<1..603)
                     /locus_tag="VHE37_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HVT35500.1"
                     /translation="MTARHVYFLSDGTGITAETLGNTLLTQFASAEFRKMTLPFINTA
                     EKARRTVDYINFAAAKNGGRPIVFSTTVNDEVRGILREVHGLFLDLFDLFIHSIEAEL
                     GEKSTHAQGRAHGLADTGRYNARIAAMNYSMEHDDGQSTRDLSRADIILVAPSRCGKT
                     PTTLYLALQHGLFATNFPLTEDDLEKQRLPAVLAGLEAKCF"
     gene            682..3060
                     /gene="ppsA"
                     /locus_tag="VHE37_07955"
     CDS             682..3060
                     /gene="ppsA"
                     /locus_tag="VHE37_07955"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HVT35501.1"
                     /translation="MSKNVLWYSELGMHDVEIVGGKNASLGEMIRNLAKSGVRVPDGY
                     ATTAAAYREFLSHEGLAERINAQLKALNIDDVQALAAAGKSIRAAVIKAPFPAALEQE
                     IRAAYDKLVAQSGAEISVAVRSSATAEDLPDASFAGQQETFLNVQGIDAVLLAIKEVF
                     ASLYNDRAIAYRVHHGFEHAQVALSAGVQRMVRSDRGASGVMFTMDTESGFREAVFIT
                     ASYGLGEAVVQGAVNPDEFYAYKPNLKAGRPAVLKRGLGEKAKKMVYTADRTLGKTVE
                     FVPVPAAERNKFCLADAEVEALARQALIIEQHYGRPMDIEWGKDGIDGQLYILQARPE
                     TVKSRAAANVLRRYKLKKKGEVLVTGRAIGQKIGAGTVRLLNSLDQMNRVRDGDVLVT
                     DMTDPDWEPIMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATQKLKDGAEVTV
                     SCAEGDTGHVYAGHVDFAVDEIHLDRMPEIPLKIMMNVGTPEQAFDFAALPHKGVGLA
                     RLEFIINRQIGIHPQALLELDKQSPDLRRVIDQMIAPYGDPLDYFVKRLVEGIATIAA
                     AFAPEPVIVRLSDFKSNEYANLVAGKSYEPHEENPMLGFRGASRYISKSFRPCFDLEC
                     RALKYVRDDMGLTNVKVMVPFVRTLEEAKRVGEILKENGLERGKNGLQLIMMCELPSN
                     AVLADKFLDHFDGFSIGSNDMTQLTLGLDRDSGLIAHLFDERDPAVKAMLHLAISACR
                     KRGKYIGICGQGPSDHPDLARWLLDEGIESISLNPDTVVETWMFLANGKVAH"
BASE COUNT          606 a         1040 c         1068 g          548 t
ORIGIN      
        1 gaaacatttc gcctccagcc ccgccagcac ggccggcagg cgctgcttct ccaggtcgtc
       61 ttcggtcagc gggaagttgg tggcgaacaa tccatgctgg agcgcgaggt acagcgtggt
      121 tggcgtcttg ccgcagcgcg aaggcgccac caggatgatg tcagcgcgcg acaggtcgcg
      181 cgtcgactgg ccgtcgtcgt gctccatcga gtagttcatc gctgcgatgc gcgcgttgta
      241 gcggccggtg tcggccaggc cgtgcgcgcg gccctgcgcg tgcgtggatt tttcgccgag
      301 ctcggcctcg atgctgtgga tgaacaggtc gaacaggtcc aggaacaggc catgcacctc
      361 gcgcaggatg ccgcgaacct cgtcgttcac cgtggtggag aacacgatcg gacgcccgcc
      421 gttcttggcg gcggcgaaat ttatataatc caccgtgcgc ctcgccttct ccgccgtatt
      481 gatgaacggc agggtcatct tccggaactc ggccgaggca aattgcgtca gcagggtgtt
      541 gcccagcgtc tcggcggtga tgccggtgcc gtcggacagg aaatacacgt gccgggcggt
      601 catgcgtgaa cctgctgctc cttcgttggg atgagtcgcg cgcagcgcgc gcggatgaga
      661 atgacacgaa acgggaatcc tgtgagcaag aacgtcctct ggtactccga gctgggcatg
      721 cacgacgtgg aaatagtcgg cggcaagaat gcctcgctcg gcgaaatgat ccgcaacctg
      781 gcgaagtccg gcgtgcgcgt gcccgacggc tacgccacca ccgccgcggc ctaccgcgaa
      841 ttcctctcgc acgaaggcct ggccgagcgc atcaacgcgc agctcaaggc gctcaacatc
      901 gatgacgtgc aggcactggc cgccgcgggc aagtcgatcc gcgcggcggt aatcaaggcg
      961 cccttccccg ccgcgctcga gcaggaaatt cgcgcggcct acgacaagct ggtcgcgcag
     1021 tccggcgcgg agatttccgt ggccgtgcgc tcgtcggcca cggccgaaga tctgccggac
     1081 gcctcgtttg ccggccagca ggaaactttc ctcaacgtgc agggcattga cgcggtgctg
     1141 ctggcgatca aggaagtgtt cgcctcgctg tacaacgatc gcgccatcgc ttaccgcgtg
     1201 catcacggct tcgagcatgc gcaggtggcg ctatccgccg gcgtgcaacg catggtgcgc
     1261 agcgaccgcg gcgccagcgg cgtgatgttc accatggata ccgaatccgg tttccgcgaa
     1321 gcagtgttca tcaccgcaag ctacggcctg ggcgaagccg tcgtgcaggg cgcggtgaat
     1381 cccgatgagt tctacgcgta caagccgaac ctgaaggccg ggcgacccgc ggtactcaag
     1441 cgcggcctgg gcgagaaggc caagaagatg gtttacacgg cagatcgcac gctgggcaag
     1501 acggtggagt tcgtgccggt gcctgcagcc gagcgcaaca aattctgcct ggccgatgcg
     1561 gaagtcgaag cgctggccag gcaggcactg atcatcgagc agcactacgg ccggccgatg
     1621 gacatcgagt ggggtaagga cggcatcgac ggccagctgt acatcctgca ggcgcggccg
     1681 gagacggtga aatcacgcgc cgccgccaat gtactgcgcc gctacaagct gaagaaaaag
     1741 ggcgaagtgc tggtcaccgg gcgcgccatc ggccagaaga tcggcgccgg caccgtgcgc
     1801 ctgctcaact cgctggacca gatgaaccgc gtgcgcgacg gcgatgtgct ggtcaccgac
     1861 atgaccgatc ccgactggga gccgatcatg aagcgcgcgt ccgccatcgt caccaaccgc
     1921 ggcggccgca cctgccacgc ggcgatcatc gcgcgcgagc tcggcattcc cgccgtggtc
     1981 ggctgcggcg acgccacgca gaagctgaag gacggcgccg aagtcaccgt gagctgcgcc
     2041 gagggcgata ccggccacgt ctatgctggc cacgtcgact ttgctgtgga cgagattcat
     2101 cttgaccgca tgcccgagat cccgctgaag atcatgatga atgtcggcac gccggagcag
     2161 gcgttcgact tcgccgcgct gccgcacaag ggcgtgggcc tggcgcgcct ggagttcatc
     2221 atcaaccggc agatcggcat ccacccgcag gcgctgctcg aactcgacaa gcagtcgccc
     2281 gacctgcgcc gcgtgatcga ccagatgatc gcgccgtacg gcgatccgct agattacttc
     2341 gtcaaacgcc tggtggaagg tatcgccacc attgccgcgg cgtttgcgcc ggaaccggtg
     2401 atcgtaaggc tgtccgattt caagtcgaac gagtacgcca acctggtcgc cggcaagagc
     2461 tatgagccgc acgaggaaaa tccgatgctg ggtttccgtg gcgcctcacg ttacatctcc
     2521 aagtcgttcc gaccctgctt cgaccttgag tgccgcgcgc tcaaatacgt gcgcgacgac
     2581 atgggcctga ccaacgtcaa ggtgatggtg ccgttcgtgc gcacgctgga ggaggccaag
     2641 cgcgtgggcg aaatcctcaa ggaaaacggg ctggagcgcg gcaagaacgg tctgcagctg
     2701 atcatgatgt gcgagctgcc gagcaatgcg gtgctggccg acaagttcct cgatcacttc
     2761 gacggcttct ccatcggctc caacgacatg acccagctga cactggggct cgatcgtgat
     2821 tccgggctga tcgcgcacct gttcgatgag cgtgatcccg cggtaaaggc gatgctccac
     2881 ctggccatct cggcctgccg caagcgcggc aagtacatcg gcatctgcgg ccaggggcca
     2941 agcgatcacc ccgatctcgc gcgctggctg ctcgacgagg gcatcgagtc gatttcgctc
     3001 aacccggaca cggtggtcga aacctggatg ttcctggcca acggcaaggt ggcgcactga
     3061 tgcgcctctg gatggccgcc tgcgcactcg cactatgcgt gactgcacac gcggatgatg
     3121 caagcccgcc gcccttcgac tactgggtgc tctcgttgtc gtggagcccg cagtactgcc
     3181 tcgacaacgc gcgcgagatc gagtgcggcc ggacctactc cttcgtggtc cacgggctgt
     3241 ggccggagcg cgaagacagc aa
//
LOCUS       DASZZC010000371         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_546387,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000371 DASZZC010000000
VERSION     DASZZC010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546387"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VHE37_07960"
     CDS             <1..1220
                     /locus_tag="VHE37_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVT35502.1"
                     /translation="TAGHPESAARDHYDNGTLVTPPDDFYSTDYYTGKLIDYIGHDRG
                     DGRPFFAYLAFQAVHFPLQVPDAYLDLYKGKYDAGYAVIRAARIAKQKELGIIPQDFQ
                     ESPGDEATMIRFGQPGVLLNDPWEKLSASDQQSEARVMEIFAGMLSNVDDNVGKLIDY
                     LKQAGVYDNTLIVFVSDNGADGMGYGFIPYTDTEDPTSVAGIDNSLANYGRPSSFVFR
                     STRWAEVGTAPFRLFKGFTAEGGIGVPAIVHLPGANPGAGNAVLSGLKDVVPTVLELA
                     GVADTGAQYGGHAVAAIEGRSLMPALSGSDAQVHADAEVFADEVNDIRYVRRGPWKMT
                     RVVNYMVPSAAEFIDHDWQLYNLDTDRGETTDVSAANPDIVAELKADWAAYVTRVNAV
                     NPVLPPIVTPMDQ"
     gene            1248..2210
                     /locus_tag="VHE37_07965"
     CDS             1248..2210
                     /locus_tag="VHE37_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HVT35503.1"
                     /translation="MKSAAPLFALAFLLAACGRVSVPDAGLGNREKCSAYSGLPAGWG
                     ADAHAGMVQVASGRFMFGSTRGYTDEGPEQPADVNGFWIDRTEVTNAQFDAFVRATGY
                     VTEAERGGGAAVFRAPDVNAPMTPGSWWQLDAHADWRHPEGAASGIAQRMHEPVVDVT
                     YADAQAYARWLHHALPSEVQWEYAARAGRSNEISDAAVRDADGRPLANFWQGVFPLQD
                     RADDGYAGRAPVGCYPDNGWRLYDMVGNVWEWTTDADGAHRIAPTLETAALTRHGQAQ
                     RYVIKGGSWLCSADYCARARASSRQAQEADLGATHLGFRTIASD"
     gene            2283..>2808
                     /locus_tag="VHE37_07970"
     CDS             2283..>2808
                     /locus_tag="VHE37_07970"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HVT35504.1"
                     /translation="MKRACLLMLALCSVAARADEGADQMLKCMHANVPPTLRVQQIEL
                     TVTGREGDDRKLTGRLYAAPDAQGRLQAMLKIDAPADLAGAAYLVHESDDYLKDGMYV
                     YLSSVKRVRRVSGTFADGALLGTHFSYNDFRQLQTAFAGTPATLEAGEDNLDGRPARI
                     LDFTPGADSRYSRVR"
BASE COUNT          466 a          979 c          956 g          407 t
ORIGIN      
        1 cgactgcggg ccaccctgaa tccgccgcgc gcgatcacta cgacaacggc acgctggtga
       61 cgccgccgga cgacttctat tccaccgact actacaccgg caagctgatc gactacatcg
      121 gccatgaccg cggcgacggc cgcccgttct tcgcctacct tgccttccag gcggtgcatt
      181 tcccgctgca ggtgcccgac gcctatctcg acctgtacaa gggcaagtac gacgccggtt
      241 acgcggtgat ccgcgccgcg cgcattgcca agcagaagga gctcggcatc atcccgcagg
      301 atttccagga gagccccggc gacgaagcga ccatgatccg cttcggccag ccgggcgtgc
      361 tgctcaacga cccgtgggag aagctgtccg ccagcgacca gcagagcgag gcgcgcgtga
      421 tggaaatctt cgccggcatg ctgagcaacg tggacgacaa cgtcggcaag ctgatcgact
      481 acctcaagca ggccggcgtc tacgacaaca cgctcatcgt cttcgtctcc gacaacggcg
      541 ccgacggcat gggctacgga ttcattccgt acaccgacac cgaggacccg accagcgtcg
      601 ccggcatcga caactcgctg gccaactacg gcaggcccag ctcgttcgtg ttccgcagca
      661 cgcgctgggc cgaggtgggc accgcgccgt tccgtctgtt caagggcttc accgcggaag
      721 gcggcatcgg cgttccggcc atcgtgcatc tgcccggcgc caatccgggc gcgggcaacg
      781 cggtgctgtc ggggctgaag gacgtggtgc cgacggtgct ggaactggcc ggcgtcgccg
      841 acaccggcgc gcagtacggc ggacacgcgg tcgccgccat cgagggccgc tcgctgatgc
      901 cggccctgag cggcagcgac gcgcaggtgc acgcggacgc ggaagtattt gccgacgaag
      961 tgaacgatat ccgctacgtg cgccgcgggc cgtggaagat gacgcgcgtg gtgaactaca
     1021 tggtgccgtc ggcggcggag ttcatcgacc acgactggca gctgtacaac ctcgacaccg
     1081 accgcggcga aaccaccgac gtgtccgccg ccaacccgga catcgtggcc gagctgaagg
     1141 ccgactgggc tgcgtacgtg acgcgggtga atgcggtgaa cccggtgctg ccacccatcg
     1201 tgacgccgat ggaccagtga tcggcgcgcg cgggcaataa tgcgcagatg aaatctgccg
     1261 cgccgctgtt cgcacttgcg ttcctgctcg ccgcgtgcgg gcgcgtgtcg gtgccggacg
     1321 ctggcctcgg caaccgcgag aaatgcagcg cctattcggg cctgcccgcc ggctggggtg
     1381 cggatgcgca cgccggcatg gtgcaggtgg cgtccggccg cttcatgttc ggcagcacgc
     1441 gcggctacac cgacgaaggt cccgagcagc cggcggatgt aaatggcttc tggatcgacc
     1501 gcaccgaggt gaccaacgcg cagttcgacg cattcgtgcg cgcgaccggc tacgtcaccg
     1561 aggccgagcg cggcggcggc gcggcggtgt tccgcgcgcc cgacgtgaac gcgccgatga
     1621 cgccgggcag ctggtggcag ctcgacgcgc atgccgactg gcgtcacccg gaaggcgccg
     1681 ccagcggcat cgcgcagcgc atgcacgagc cggtggtgga tgtgacctat gccgacgcgc
     1741 aggcctacgc gcgctggctg caccatgcgc tgcccagcga ggtgcaatgg gaatacgccg
     1801 cgcgcgccgg ccgcagcaac gaaatctccg acgccgcggt gcgcgacgcc gacggccggc
     1861 cgctggccaa tttctggcag ggcgtgttcc cgctgcagga tcgtgccgac gacggctacg
     1921 ccggccgcgc gccggtgggc tgctatcccg acaacggctg gcgactgtac gacatggtcg
     1981 gcaacgtgtg ggagtggacc accgacgccg acggcgcgca tcgcatcgcg cccacgctgg
     2041 agacggccgc gctcacgcga catggccagg cgcagcgcta cgtgatcaag ggcggctcgt
     2101 ggttgtgctc ggccgattac tgcgcgcgcg cgcgcgcctc ctcgcgccag gcgcaggaag
     2161 cggatctggg cgccacccac ctgggtttcc gcacgattgc ttcggactag ctgcgccaat
     2221 cggacgaggg cgcagggtcg cgccgcgcta agatcaggcg catggcacac cggagaacaa
     2281 caatgaagcg cgcctgcctg ctgatgcttg ccctgtgttc tgtcgcggcc cgcgccgatg
     2341 agggcgccga ccagatgctc aagtgcatgc atgccaacgt gccgccgacg ctgcgcgtgc
     2401 agcagatcga gctcaccgtc accggccgcg aaggcgacga ccgcaagctc accggccgcc
     2461 tgtacgccgc gcccgacgcg cagggccggc tgcaggccat gctcaagatc gatgcgccgg
     2521 ccgatctcgc cggcgccgcc tacctggtgc acgagtccga cgactacctc aaggacggca
     2581 tgtacgtgta cctgtcgtcg gtcaagcgcg tgcgccgcgt gtccggtacc tttgccgatg
     2641 gcgcgctgct cggcacgcat ttctcgtaca acgatttccg ccagctgcag accgcgttcg
     2701 ccggcacgcc ggccacgctg gaagccggcg aggacaacct cgacggccgg cccgcgcgca
     2761 tcctggattt cacgcccggg gcggactcgc gctattcgag agtgcgcg
//
LOCUS       DASZZC010000372         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1249023,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000372 DASZZC010000000
VERSION     DASZZC010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249023"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..177
                     /locus_tag="VHE37_07975"
     CDS             4..177
                     /locus_tag="VHE37_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVT35505.1"
                     /translation="MSETFTTEPLGWAVRKGDPDFLNWLNNFLRTIRADGSYDALYKK
                     WFENTAWLQQVTN"
     gene            183..1130
                     /locus_tag="VHE37_07980"
     CDS             183..1130
                     /locus_tag="VHE37_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVT35506.1"
                     /translation="MQQHPRRWLWHGVYALVLVALGVAVYQASERVDYIWRWERLPQY
                     VISTEGEELRAPFDGFVALAADGKSITLSELKGARKETITGFDEVLVGEGDLVFQNDA
                     LARKKGLRAGPLLVGLGLTLEISALSLVCALIIGLLAGLARVSHNPAARNLAATYVEI
                     IRGTPLLVQIFIFYFFIGTVLNLSAFTAGVAALSVFTGAYVAEIIRAGIEGVPRGQSE
                     AARSLGMTQLQTLRHVVLPQALRRSLPPLAGQFISLIKDSSLVSVMALTDLTKAGREV
                     VSSTFSPFEVWFTVAALYLLLTGALSWVVRRLEKRYAHG"
     gene            1135..1863
                     /locus_tag="VHE37_07985"
     CDS             1135..1863
                     /locus_tag="VHE37_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVT35507.1"
                     /translation="MIDVRGVEKRYANGALGLRNASLTVRRGEVVCIVGPSGSGKSTL
                     LRAINALEPISAGEIYVDGIAVHDHRTDLNKLRTHAGMVFQQFNLFPHKTVLQNLVLA
                     PCEVLRLTQADSAARAQALLAKVGLSGFEQRYPAQLSGGQQQRVAIARALAMQPAIML
                     FDEPTSALDPELVGEVLDVMKQLATEGMTMCVVTHEMSFARRMAHRVVFMDGGDVVYQ
                     APPQEFFGAPKNERLKQFLARVSQ"
     gene            complement(1945..2163)
                     /gene="infA"
                     /locus_tag="VHE37_07990"
     CDS             complement(1945..2163)
                     /gene="infA"
                     /locus_tag="VHE37_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVT35508.1"
                     /translation="MAKEDHIEMNGIVAETLPNTTFRVKLENGHVVTAHISGRMRKNY
                     IRILTGDKVKVELTPYDLTKGRITFRDK"
     gene            complement(2237..2944)
                     /locus_tag="VHE37_07995"
     CDS             complement(2237..2944)
                     /locus_tag="VHE37_07995"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HVT35509.1"
                     /translation="MSEPQRVRLYLSTGHACGYLSGLEARNAYLDPDFPMSPQRYGWL
                     LAQGFRRSGVHVYRPHCPQCHACQPARVPVADFVPSRTQRRCLARNDDLQLTVTRELG
                     EEHFELYQRYLAVRHPGGGMDSGNREAFHLFLECGWGDAQFWEFRVGARLLALAVVDR
                     VPSALSAVYTFFDPDEGARSLGTLAVLRQIEAAAEQRLDHVYLGYWVRGSAKMDYKRN
                     FRPLELLGTGGWHALES"
     gene            complement(2950..3693)
                     /gene="aat"
                     /locus_tag="VHE37_08000"
     CDS             complement(2950..3693)
                     /gene="aat"
                     /locus_tag="VHE37_08000"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HVT35510.1"
                     /translation="MDNPIRLHWLDPRNPQQPFPPVHLAMRDPNGLLAIGGDLSLPRL
                     LRAYSQGIFPWFNPDEPILWWCPNPRAVLAPGEVHASHSLRKRVRKGGYAITFDRAFA
                     EVLDACSAARAKSRGTWLGVDMKCAYYDLHRSGHCHSVEVWREGQLIGGLYGVSLGKA
                     FFGESMFSRATDGSKLAFYHLCRQLDAWKFELVDCQISSAHLATLGARDIPREDFLKR
                     LRHAVNHASRAGPWRFDVELPDGTGHLPA"
     gene            complement(3723..4055)
                     /locus_tag="VHE37_08005"
     CDS             complement(3723..4055)
                     /locus_tag="VHE37_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35511.1"
                     /translation="MSMQTRHFVLADDGIREFSAEQAATIAAGGGRLPEFAGRNVRYL
                     QLTVNDEISDGEIKVQTSGASIRFDNDGRISGAEPPGEAQQISRFEHDAVVQWALKNL
                     PAVAPTFH"
BASE COUNT          673 a         1343 c         1428 g          723 t
ORIGIN      
        1 cacctgtcgg agactttcac caccgagccg ctgggctggg cggtgcgcaa gggcgatccc
       61 gactttctca actggctgaa caatttcctc cgcaccatcc gcgccgacgg cagctacgac
      121 gcgctgtaca agaaatggtt cgagaacacc gcctggctgc aacaggtcac caactgatcc
      181 ccgtgcagca acatccccgc cgctggctgt ggcacggcgt ctacgcgctg gtgctggtgg
      241 cgcttggcgt cgcggtctac caggccagcg agcgcgtcga ctacatctgg cgctgggaac
      301 ggctgccgca atacgtcatc tctaccgagg gcgaggaact gcgcgcgccg tttgacggct
      361 tcgtcgcgct ggcggccgac ggcaagtcga tcacgctgtc cgaactcaag ggcgcacgca
      421 aggaaaccat caccggtttc gatgaagtgc tggtgggcga gggcgacctg gtgttccaga
      481 acgacgcgct ggcgcgcaag aagggtttgc gcgccggccc gctgctggtc gggctgggcc
      541 tgacgctgga gatttccgcg ctgtcgctgg tgtgcgcgct gatcatcggc ctgctcgccg
      601 ggctggcgcg cgtctcgcac aacccggccg cgcgcaacct ggcggcgact tacgtggaga
      661 tcatccgcgg cacgccgctg ctggtgcaga tcttcatctt ctacttcttc atcggcacgg
      721 tgctgaatct ttccgccttc accgccggcg tcgccgcact gtcggtgttc accggcgcct
      781 acgtggcgga gatcatccgc gccggcatcg agggcgtgcc gcgcgggcag agcgaggccg
      841 cgcgctcgct gggcatgacg cagctgcaga cgctgcgcca cgtggtgctg ccgcaggcgc
      901 tgcgccgttc gctgccgccg ctggccgggc agttcatctc gctgatcaag gattcgtcgc
      961 tggtgtcggt gatggcgctc accgacctga ccaaggccgg gcgcgaagtg gtgagcagca
     1021 ccttcagccc gttcgaggtg tggttcaccg tggccgcgct gtacctgctg ctgaccggcg
     1081 cgctgtcgtg ggtggtgcgg cggctggaaa agagatacgc ccatggctga cgcattgatc
     1141 gacgtgcgcg gcgtggagaa gcgctatgcc aatggcgcgc tcggcctgcg caacgcctcg
     1201 ctcaccgtgc ggcgcggcga ggtggtgtgc atcgtcggac ccagcggttc gggcaagtcg
     1261 acgctgctgc gcgcgatcaa cgcgctggag ccgatcagcg ccggagaaat ctacgtcgac
     1321 ggcatcgcgg tgcacgatca ccgcaccgat ctcaacaagc tgcgcacgca cgccggcatg
     1381 gtgttccagc agttcaacct gtttccgcac aagacggtgc tgcagaacct ggtgctggcg
     1441 ccgtgcgagg tgctgcgcct gacgcaggcg gacagcgcag cgcgtgcgca ggccttgctg
     1501 gccaaggtgg gcctgtcggg tttcgagcag cgctatccgg cacagctgtc gggcggccag
     1561 cagcagcgcg tagccatcgc gcgcgcgctg gccatgcagc cggccatcat gctgttcgac
     1621 gagccgacct cggcgctcga tcccgagctg gtcggcgaag tgctcgacgt gatgaagcag
     1681 ctggccaccg agggcatgac catgtgcgtg gtgacgcacg agatgagctt cgcgcggcgc
     1741 atggcgcacc gcgtggtgtt catggatggc ggcgacgtgg tgtaccaggc gccgccgcag
     1801 gaattcttcg gtgcgccgaa gaacgagcgg ctgaagcagt tcctggcaag ggtgtcgcag
     1861 tagcaggaac gttgccgtcc gcggcgccat tcgctcagcc ggcgatcccc gcaagcgggg
     1921 cccctcgccg ggtggctcct ggctttattt atcgcggaaa gtgatgcggc ccttggtcag
     1981 gtcgtaaggc gtgagctcca ccttgacctt gtcgccggtg aggatgcgga tgtaattctt
     2041 ccgcatgcgg cccgagatgt gcgcggtgac cacgtggccg ttttccagct tcacgcggaa
     2101 cgtggtgttc ggcagcgttt ccgcaacgat gccgttcatc tcgatgtgat cttcttttgc
     2161 catgccaacc gtggggggcc aaaaacgagg cgcgcattat atccacgttc gcgcgcggcg
     2221 cttgcgcacg ggcggatcag ctttccaacg cgtgccagcc gcctgtgccg agcagttcca
     2281 gcgggcggaa atttctcttg tagtccatct tggcgctgcc gcgcacccag tagccgagat
     2341 agacgtgatc cagccgctgt tcggcagcgg cctcgatctg ccggagcacg gcgagcgtgc
     2401 ccagcgagcg ggcaccttca tctggatcga agaaggtgta caccgccgac agcgccgacg
     2461 gcacgcggtc gaccaccgcc aacgcgagca gccgcgcgcc gacacggaat tcccagaact
     2521 gcgcgtcgcc ccagccgcat tcgaggaaca ggtgaaaggc ctcgcggttg ccgctgtcca
     2581 tgccgccgcc gggatggcgt accgcgagat agcgctggta caactcgaaa tgctcctcgc
     2641 ccagttcgcg cgttacggtc agctgcaggt catcgttgcg cgccaggcag cgccgctgcg
     2701 tgcgcgacgg cacgaagtcg gctaccggca cgcgcgccgg ctggcaggca tggcattgcg
     2761 ggcagtgcgg ccggtagaca tgcacaccgc tgcggcggaa gccctgcgcc agcagccagc
     2821 cgtagcgctg cgggctcatg gggaaatccg ggtcgagata ggcgttgcgc gcttccaggc
     2881 cggacaggta gccgcacgcg tgcccggtgg acaggtacag gcgtacgcgc tgcggctcgc
     2941 tcatggcggt cacgccggca ggtgtcctgt gccgtcgggc agttcgacgt cgaaacgcca
     3001 ggggccggcg cgcgaagcat ggttgaccgc gtggcgcagg cgcttcagga aatcttcgcg
     3061 cggaatgtcg cgcgcgccca gcgtggccag gtgcgccgag ctgatctggc agtcgaccag
     3121 ctcgaacttc caggcgtcga gctggcggca caggtgatag aaggccagct tggagccgtc
     3181 ggtcgcgcgc gagaacatcg attcgccgaa gaacgccttg cccagcgaaa caccatagag
     3241 gccgccgatc agctggcctt cgcgccacac ttccaccgag tggcaatggc cgctgcgatg
     3301 caggtcgtaa taggcgcatt tcatgtcgac gcccagccag gtgccgcggc tcttggcgcg
     3361 cgcggccgag caggcgtcga gcacttcggc gaacgcgcgg tcgaaagtga tggcgtaacc
     3421 gcccttgcgc acgcgcttgc gcagcgagtg cgacgcgtgc acttcgcccg gcgccagcac
     3481 cgcgcgcgga ttggggcacc accacaggat cggttcgtcg ggattgaacc aggggaagat
     3541 gccctgcgaa tacgcgcgca gcaggcgcgg cagcgagagg tcgccgccga tggcgagcag
     3601 gccgttggga tcgcgcatgg ccaggtgcac cggcggaaac ggctgctgcg gattgcgcgg
     3661 gtccaaccaa tgcaggcgta tggggttatc catgacgcct caactctgcg cctttgcgcg
     3721 gctcagtgga aggttggtgc gacggccggc aggttcttca gcgcccattg caccacggca
     3781 tcgtgctcga agcggctgat ctgctgcgct tcgcccggcg gctcggcgcc gctgatgcgg
     3841 ccgtcgttgt cgaagcggat gctggcgccc gaggtctgca ccttgatctc gccgtcgctg
     3901 atctcatcgt tcacggtgag ttgcaggtag cgcacgttgc ggccggcgaa ttccggcagg
     3961 cggccgccgc cggcggcgat ggtcgccgcc tgctccgcgg aaaattcgcg tatgccgtca
     4021 tcggccagta caaaatgacg tgtctgcatg ctcatgttct ggtcaccttt caatccatat
     4081 gaccgcgaaa atatcctgag ggtttcccat ccatattcaa gaggcgtgcc gcacaaccgc
     4141 gcgccttgcg cgctgttata atttcat
//
LOCUS       DASZZC010000373         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_224294,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000373 DASZZC010000000
VERSION     DASZZC010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224294"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VHE37_08010"
     CDS             <1..290
                     /locus_tag="VHE37_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HVT35512.1"
                     /translation="QSIFAWYRAGTPEHAPVLVLCNFTPVPRIGYRVGVPRGGRWREL
                     LNSDAALYGGSNIGNAGAVEAAAVLSHGLPYALTLTLPPLAVVLLTCERCD"
     gene            443..2539
                     /gene="glgX"
                     /locus_tag="VHE37_08015"
     CDS             443..2539
                     /gene="glgX"
                     /locus_tag="VHE37_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HVT35513.1"
                     /translation="MSEAADRLHPAPAGPLGAHCDGRGIRFAVYAAGAEKVELCLFDA
                     SGRHEQRLVMPDCIDGVWHGYLPGARGDQLYGYRAYGPYEPKRGLRYNGNKLLVDPYA
                     KKLAGALRWHDALYGYRVGSPRGDLSFDRRDSAAYLPKCVACDERFEWGNDHPPQVPW
                     PETVIYEMHLRGFSMRHPEIPEHERGSFGALGHPAAIAHLKRLGITAVELLPVHAFLR
                     DRMLEEKQLTNYWGYNTLGYFAPEAAYLSDGTPAQMKWAVQQLHAAGIEVILDVVYNH
                     TCEGNELGPTLSFRGLDNASYYRLLPDDPRRYINDTGCGNTVNCSHPRVIQLIMDSLR
                     WWVSEYHVDGFRFDLGVTLGREPQGFDPGCGLFDAMRQDPLLARVKLISEPWDIGPGG
                     YQIGNHPPGFSEWNGKFRDDVRRYWKGDAGVRPALAARLQGSAEMFDRERRRPWASVN
                     FITAHDGYTLHDLVSYNGKHNQANGEDNHDGANDNESSNWGAEGPTDNPEIVNQRDKI
                     KRALLLTLLASHGTPMLLAGDEFGQTQQGNNNAYCQDNDISWLDWSRAGSEAGRALTD
                     FCARLLALRREQPLLQGREFQHARVDIAPGVRDIDWFDERGIELLPEDWNNANGRLLG
                     LRRSRVLPGGGYESIALLCNSDGAAHSFRLPEPAQRWQLLVDTHRPAPGSVDLNGNQY
                     EVAPHSAVLLHARELP"
     gene            2536..>3405
                     /locus_tag="VHE37_08020"
     CDS             2536..>3405
                     /locus_tag="VHE37_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVT35514.1"
                     /translation="MNARHEFPFGAQLLAPGRTRFSLWAPARKRVLLEIDGREPLPMA
                     AEAGGWFRLETGCAAGSCYRYRIADDLAVPDPASRAQAGDVHDCSIVVDPGAYAWQQP
                     DWRGRPWEEMVIYEIHAGVSGGFDGVRALLPHIALLGATAVELMPVADFSGRRNWGYD
                     GVLPYAPDASYGSPNDLKRLVDEAHGHGLCIYLDVVYNHFGPDGNFLQQYAPQFFDGA
                     RQSPWGAAIDFSRHQVREFYIHNALYWLNEYRFDGLRFDAVHAIDDRDFLVELARRVR
                     AGTEPGRPVHLMLE"
BASE COUNT          560 a         1222 c         1111 g          512 t
ORIGIN      
        1 gccagtcgat tttcgcctgg tatcgcgccg gcacgccgga gcacgcaccg gtgctggtgc
       61 tgtgcaactt cacgccggtg ccgcgcatcg gctaccgcgt cggcgtgccg cgcggcggcc
      121 gctggcgcga gctgctcaac agcgacgccg ccctttacgg cggatcgaac atcggcaatg
      181 ccggcgcagt cgaagctgca gcggtgctct cgcacggcct gccttacgca ctcacgctca
      241 cgctgccgcc gctggcggtg gtgctgctga cctgcgaacg ctgcgactga cgcggcagga
      301 gcgccgaaaa aacgtccacc gcaccggcat cttgtgtgtt attaaatatc gtatattttt
      361 atatttaatc aatgtttaat aacagtcaaa acagcagtgc ggttggctca aaaaatcgac
      421 ggtcatcgat gtggagccgc gcgtgagcga ggccgccgac cgcctgcacc ctgcacccgc
      481 cggaccgctc ggcgcgcatt gcgatggccg cggcatccgc tttgccgtgt acgctgccgg
      541 cgcggaaaaa gtcgagctgt gcctgttcga tgccagcggc cgccacgagc agcgcctggt
      601 catgcccgat tgcatcgacg gcgtgtggca cggctacctg cccggcgcgc gcggcgatca
      661 gctctacggc taccgcgcct acgggccata cgagcccaaa cgcggcctgc gctacaacgg
      721 caacaagctg ctggtcgatc cgtatgcgaa aaaacttgcg ggcgcgctgc gctggcacga
      781 cgcgctctac ggctaccgcg tcggctcgcc gcgcggtgac ctgtccttcg accgccgcga
      841 cagcgccgcc tacctgccca agtgcgtggc ctgcgacgaa cgcttcgagt ggggcaacga
      901 ccatccgccg caggtgccgt ggcccgagac ggtgatctac gagatgcacc tgcgcggctt
      961 ctccatgcgc cacccggaaa ttcccgagca cgagcgcggc agcttcggcg cgctgggtca
     1021 tcccgccgcc atcgcccacc tgaaacgcct gggcatcacc gcggtggagc tgctgccggt
     1081 gcatgccttc ctgcgcgacc gcatgctcga ggaaaagcag ctaaccaact actggggcta
     1141 caacacgctc ggctacttcg cgccggaggc ggcgtacctg tccgacggca cgccggcgca
     1201 gatgaagtgg gcggtgcagc agctgcacgc cgccggcatc gaggtgatcc tcgatgtcgt
     1261 ctacaaccac acctgcgagg gcaacgagct cggccccacg ctgtcgttcc gcgggctgga
     1321 caacgccagc tactaccgcc tgctgccgga cgatccgcgc cgctacatca acgacaccgg
     1381 ctgcggcaac acggtcaact gctcgcatcc gcgcgtgatc cagctgatca tggactcgct
     1441 gcgctggtgg gtcagcgaat accacgtcga cggcttccgc ttcgacctgg gcgtcaccct
     1501 cggccgcgag ccgcagggct tcgaccccgg ctgcggcctg ttcgacgcca tgcgccagga
     1561 cccgctgctg gcgcgggtga agctgatcag cgaaccctgg gacatcggcc ccggcggtta
     1621 ccagatcggc aaccacccgc caggtttctc cgagtggaac ggcaagttcc gcgacgacgt
     1681 gcgccgctac tggaaaggcg atgccggcgt gcgcccggcg ctggccgcgc gcctgcaggg
     1741 cagcgccgag atgttcgacc gcgaacggcg gcggccctgg gcgagcgtca acttcatcac
     1801 cgcgcacgac ggctacaccc tgcacgacct ggtctcgtac aacggcaagc acaaccaggc
     1861 caacggcgaa gacaaccacg atggcgccaa cgacaacgag agcagcaact ggggcgccga
     1921 ggggccgacc gacaatcccg agatcgtcaa ccagcgcgac aagatcaagc gcgcgctgct
     1981 gctcacgctg ctggcctcgc acggcacgcc gatgctgctg gccggcgacg agttcggcca
     2041 gacccagcag ggcaataaca acgcctactg ccaggacaac gacatcagct ggctggactg
     2101 gtcgcgcgcc ggcagcgagg ccgggcgcgc gctgaccgat ttctgcgcgc gcctgctggc
     2161 gctgcgccgc gaacagccgc tgctgcaggg ccgcgagttc cagcacgcgc gcgtcgatat
     2221 cgcgccgggc gtgcgcgaca tcgactggtt cgacgagcgc ggcatcgagc tgctgccgga
     2281 agactggaac aacgccaacg gccgcctgct cggcctgcgc cgctcgcgcg tgctgcccgg
     2341 cggcggctac gaatccatcg cgctgctgtg caactccgac ggcgccgcgc actccttccg
     2401 cctgcccgag ccggcgcagc gctggcagtt gctggtcgac acccaccggc cggcgccggg
     2461 cagcgtcgac cttaacggca accagtacga agtcgcgccg cacagcgcgg tgctgctgca
     2521 cgcgcgcgag ctgccatgaa cgcgcgccac gaatttccct tcggcgcaca gctgctggcg
     2581 ccgggccgca cgcgcttctc gctgtgggca ccggcgcgca agcgcgtgct gctggaaatc
     2641 gacggccgcg agccgctgcc gatggcggcc gaggccggcg gctggttccg cctggaaacc
     2701 ggctgcgccg ccggcagctg ctaccgctac cggattgcgg acgatctcgc ggtgcccgac
     2761 ccggcctcgc gcgcccaggc cggcgacgtg cacgactgca gcatcgtggt cgatcccggc
     2821 gcctatgcct ggcagcagcc ggactggcgc gggcggccgt gggaagaaat ggtcatctac
     2881 gaaatccacg ccggcgtgtc cggcggcttt gacggcgtgc gcgcgctgct gccgcacatt
     2941 gcgctgctcg gcgccaccgc ggtggaactg atgccggtcg cggatttttc cggccgccgc
     3001 aactggggct acgacggcgt gctgccctac gcgcccgacg ccagctacgg cagccccaac
     3061 gatctgaagc gtctggtcga cgaggcgcat ggccacggcc tgtgcatcta cctcgacgtg
     3121 gtctacaacc acttcggccc cgatggcaat ttcctgcagc agtacgcgcc gcagttcttc
     3181 gacggcgcgc ggcaatcgcc ttggggcgcg gccatcgact tctcgcggca ccaggtgcgc
     3241 gagttctaca tccacaacgc gctgtactgg ctcaacgagt accgcttcga tggcctgcgc
     3301 ttcgacgccg tgcacgccat tgacgaccgc gatttcctgg tggaactggc gcggcgcgtg
     3361 cgcgccggca ccgaacccgg ccgcccggtg cacctgatgc tggag
//
LOCUS       DASZZC010000374         4458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_607594,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000374 DASZZC010000000
VERSION     DASZZC010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607594"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VHE37_08025"
     CDS             <1..791
                     /locus_tag="VHE37_08025"
                     /inference="COORDINATES: protein motif:HMM:NF022448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BcsE family c-di-GMP-binding protein"
                     /protein_id="HVT35515.1"
                     /translation="LAKTVHHLRIHAGNGIKIVVRERGRRLRYNQEMLLNRLGANAIA
                     DSTLSFSRFLSLLDSLRDRSYDREVAPVFEEAVLQAVPPEQQGYLPAREFVMAVREAV
                     ARSALLGVQCALLQLVINPQQAHLDVLKRIRIGRPGDLFTADDRCVYLFLFACREPDI
                     DTALNRMVGMAQTELFDAQLRNADQDSINVALLALERHLRLSSPVDYSALLATPAAAP
                     APAPAAAAVPAPEPTIAVPREYQVLAMPAAPPRTAVREPLPVRT"
     gene            788..979
                     /locus_tag="VHE37_08030"
     CDS             788..979
                     /locus_tag="VHE37_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35516.1"
                     /translation="MNTLAAFDPWLIAAIALITALLVVLALWLAASRTGRRLRRLLDR
                     DWQPVFMQPWHPPKDGSAQ"
     gene            979..1728
                     /gene="bcsQ"
                     /locus_tag="VHE37_08035"
     CDS             979..1728
                     /gene="bcsQ"
                     /locus_tag="VHE37_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090540 - bacterial cellulose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HVT35517.1"
                     /translation="MQVIAVVSAAGGSGRTTLTAALAALLAMRGRRVLALDLDPRNSL
                     GMQLGLPLEEADGWAPRFLAGGNWNQAAFRNSDGVQVVPFGRVPPQKLPAIEELALGA
                     PDWLQRRLNQLALPDDAILLIDTDRLPSGYALQAINAAQVILNVLLPDAAAPGAARYA
                     ELAAGSRPVHHVVNQLEALRPLQHDLMQLLRQELGGRLASYSVHRDPAVPEAVASNRG
                     LTAYAPNGQAAHDLQGLANWLLDLLGAGAHA"
     gene            1725..4259
                     /gene="bcsA"
                     /locus_tag="VHE37_08040"
     CDS             1725..4259
                     /gene="bcsA"
                     /locus_tag="VHE37_08040"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HVT35518.1"
                     /translation="MNPLAQRLARTLGVKHAGRLREWLLRIFVLPPEQARPWPRAVVL
                     LVAPLAQLLARALGVRRLDDPREWLLRIFVLPQRLPPPPVRVFEPLLRLLWRAMRRAL
                     HIALWPWRWLVRRVSQIDFIAAEERFEDAGDALAGSHRLVRVLLLAASALLFYVTATM
                     PLTHAEQCLFLALMWAVAVVMKQMPGSLPTLALVMLSLLTSARYIWWRVSQTLLLEDP
                     LDYLFGIGLVSAEAYTWLVMILGYFQNAWPLKRKPVPLPADTSSWPTVDVFIPTYNEP
                     LKVVKPTVFAAQGLDWPRDKLNIHILDDGRRAEFREFAREAGVNYVIRNNNAHAKAGN
                     INAALKNTHGEFIAIFDCDHVAVRSFLQTTMGWMLRDPNCAMIQTPHHFFSPDPFEKN
                     LGTFRQVPNEGTLFYGLVQDGNDLWNSTFFCGSCAVIRRAPLEQIGGIAVETVTEDAH
                     TALRLQRLGWNTAYLNLTQAAGLATESLSSHIGQRIRWARGMAQIFRIDNPLFGRGLK
                     LFQRLCYTNAMLHFFHGIPRIVFLTAPLSYLYFQLHVINATAFAIGIYALPHIIMSQV
                     ANSRMQGRYRHSFWAEVYESVLAWYIMLPTTLAFFNPKLGKFNVTAKGGLVEQSYFDW
                     TISRPYIALIIANLGGVAIGIGRLLWWNTFEISTVVMNLLWALYNLVMLGTTVGIAEE
                     SRQVRQAHRVPMRIPATVVFADGRSVVCRTDDYSTNGLGLQWPAGEPLPAPRTQLQVV
                     LSRGDEEHFFTAMVTGAHGLRLGLRFDGLTLEDERNLVQCTFGRADAWVDWRDPQTQD
                     RPLDSLGEVLLFGLNGYLRLIKRGLAAWSQWRQQAREARTAPREIV"
BASE COUNT          662 a         1552 c         1491 g          753 t
ORIGIN      
        1 aactggccaa gaccgtgcac cacctgcgca ttcatgccgg caacggcatc aagatcgtgg
       61 tgcgcgaacg cggccgccgc ctgcgctaca accaggagat gctgctcaac cggctgggcg
      121 ccaacgccat cgccgactcc accctgtcgt tctcgcgctt cctctcgctg ctcgattcgc
      181 tgcgcgaccg cagctacgac cgcgaggtgg cgccggtgtt cgaggaggcc gtgctgcagg
      241 cggtgccgcc cgagcagcag ggctacctgc cggcgcgcga attcgtcatg gcggtgcgcg
      301 aggcggtggc gcgctcggcc ctgctcggcg tgcagtgcgc gctgctgcag ctggtgatca
      361 atccgcagca ggcccatctc gacgtgctca agcgcatccg catcggccgg ccaggtgacc
      421 tgtttaccgc agacgaccgc tgcgtctacc tgttcctgtt cgcctgccgc gagccggaca
      481 tcgacaccgc gctgaaccgc atggtcggca tggcgcagac cgaattgttc gacgcccagc
      541 tgcgcaatgc cgaccaggac agcatcaacg tggcgctgct ggcgctggag cggcacctgc
      601 ggctgtcgtc gccggtcgac tacagcgcgc tgctggccac gcccgccgcg gcaccggcgc
      661 ccgcgcccgc ggcggccgcg gtacctgcac ctgaaccgac aatcgccgtg ccgcgcgagt
      721 accaggtgct ggccatgccc gcggcgccgc cgcgcacggc ggtgcgcgaa cctctgccgg
      781 tgcgcacgtg aacacgctcg ccgctttcga cccatggctg atcgccgcca tcgcgctgat
      841 caccgcgctg ctcgtggtgc tggcgctgtg gctggcggcc agccgcaccg ggcgccggct
      901 gcggcgcctg ctcgaccgcg actggcagcc ggtgttcatg cagccgtggc atccgccgaa
      961 agacgggagt gcgcagtgat gcaggtgatc gccgtggtgt ccgccgccgg cggcagcggc
     1021 cgcaccacgc tcaccgccgc gctggctgcg ctgctggcca tgcgcgggcg gcgcgtgctg
     1081 gcgctggatc tcgatccgcg caacagcctg ggcatgcagc tgggcctgcc attggaggaa
     1141 gccgatggct gggcgccgcg cttcctggcc ggcggcaact ggaaccaggc tgcgttccgc
     1201 aactccgacg gcgtgcaggt ggtgccgttc ggccgcgtgc cgccgcagaa gctgccggcg
     1261 atcgaggaac tggcgctcgg cgcgcctgat tggctgcagc ggcgcctgaa ccagctggcc
     1321 ttgccggacg atgcgatcct gctgatcgac accgaccgcc tgccctcggg ctatgcactg
     1381 caggcgatca atgccgcgca ggtgatcctc aacgtgctgc tgcccgacgc cgctgcgccg
     1441 ggcgccgcac gctacgcgga actggctgcg ggctcgcggc cggtgcacca cgtggtcaac
     1501 cagctggaag ccctgcgccc gctgcagcac gacctgatgc agctgctgcg acaggaactg
     1561 ggcgggcgcc tggccagcta ctcggtgcac cgcgatccgg cggtgccgga agcggtcgcc
     1621 agcaaccgcg ggctcaccgc gtatgcaccc aacggccagg ccgcgcacga cctgcagggc
     1681 ctggccaact ggctgctgga tttgctcggc gccggagcgc atgcatgaat ccgctggctc
     1741 agcggctggc gcgcacgctt ggcgtgaagc acgccgggcg cctgcgcgag tggctgctgc
     1801 gcatcttcgt gctgccgccg gaacaggcgc ggccctggcc gcgcgcggtc gtgctgttgg
     1861 tcgcgccgct ggcgcagctg ctggcgcgcg cgctcggcgt gcgcaggctc gacgatccgc
     1921 gcgaatggct gctgcgcatc ttcgtgctgc cgcagcgact gccgcctccg ccggtgcgtg
     1981 tgttcgagcc gctgctgcgg ctgctgtggc gcgcaatgcg gcgtgcattg catatcgcct
     2041 tgtggccctg gcgctggctg gtgcggcgcg tgtcgcagat tgacttcatc gccgccgagg
     2101 aacgtttcga ggatgccggc gacgccctgg ccggcagcca ccggctggtg cgcgtgctgc
     2161 tgctggcggc ctcggcgctg ctgttctacg tgaccgccac catgccgctg acgcacgcgg
     2221 agcaatgcct gttcctggcc ctgatgtggg cggtggcggt ggtgatgaag cagatgccgg
     2281 gctcgctgcc gacgctggcc ctggtcatgc tcagcctgct gaccagcgcg cgctacatct
     2341 ggtggcgcgt gagccagacg ctgctgctgg aagacccgct cgactacctg ttcggcatcg
     2401 gcctggtgag cgcggaggcc tacacctggc tggtcatgat cctgggctac ttccagaacg
     2461 cgtggccgct caagcgcaag ccggtgccgc tgccagccga cacctccagc tggccgacgg
     2521 tcgacgtgtt cattcccacc tacaacgagc cgctgaaggt ggtgaagccc accgtgttcg
     2581 ccgcgcaggg cctggactgg ccgcgcgaca agctcaacat ccacatcctc gacgacggcc
     2641 ggcgcgcgga gttccgcgag tttgcgcgcg aggccggtgt gaactacgtg atccgcaaca
     2701 acaacgcgca cgccaaggcc ggcaacatca acgccgcgct gaagaatacg cacggcgagt
     2761 tcatcgccat cttcgactgc gatcacgtgg cagtgcgctc gttcctgcag accaccatgg
     2821 gctggatgct gcgcgacccc aactgcgcca tgatccagac gccgcaccat ttcttctcgc
     2881 ccgatccgtt cgagaagaac ctgggcacct tccgccaggt gcccaacgag ggcacgctgt
     2941 tctacggcct ggtgcaggac ggcaacgacc tgtggaactc caccttcttc tgcggctcct
     3001 gcgcggtgat ccggcgcgca ccgctggagc agatcggcgg catcgcggtg gagacggtga
     3061 ccgaggacgc gcacaccgcg ctgcgcctgc agcggctggg ctggaacacg gcgtacctga
     3121 atctcaccca ggccgcggga ctggccaccg aatcgctgtc gagccacatc ggccagcgca
     3181 tccgctgggc gcgcggcatg gcgcagatct tccgcatcga caacccgctg ttcggacgcg
     3241 gcctgaagtt gttccagcgc ctgtgctaca ccaacgcgat gctgcatttc ttccacggca
     3301 tcccgcgcat cgtgttcctt accgcgccgc tgtcgtatct ctacttccag ctgcacgtga
     3361 tcaacgcgac tgccttcgcc atcggcatct acgcgctgcc gcacatcatc atgtcgcagg
     3421 tggccaactc gcgcatgcag ggacgctatc gtcactcgtt ctgggccgag gtctacgagt
     3481 cggtgctggc ctggtacatc atgctgccga ccacgctggc cttcttcaat ccaaagctgg
     3541 gcaagttcaa cgtcaccgcc aagggggggc tggtggagca gagctatttt gactggacca
     3601 tctcgcgccc gtacatcgcg ctgatcatcg ccaacctggg cggcgtcgcc atcggcatcg
     3661 gccggctgct gtggtggaac accttcgaga tcagcactgt ggtgatgaac ctgctgtggg
     3721 cgctgtacaa cctggtgatg ctgggcacca ccgtgggcat cgccgaggaa tcgcgccagg
     3781 tgcggcaggc acaccgcgtg ccgatgcgca tccccgccac ggtggtgttc gccgacggcc
     3841 gcagcgtggt gtgccgcacc gacgactact ccaccaacgg cctgggcctg cagtggcccg
     3901 cgggcgagcc gctgccggcg ccgcgcacac agctgcaggt ggtgctgtcg cgcggcgacg
     3961 aagagcattt cttcaccgcc atggtcaccg gcgcgcacgg cctgcgcctg ggcctgcgct
     4021 tcgacgggct gacccttgaa gacgagcgca acctggtgca atgcaccttc ggacgcgccg
     4081 atgcctgggt ggactggcgc gatccgcaga cgcaggaccg tccgctcgac agcctcggcg
     4141 aggtgctgct gttcgggctc aacggctacc tgcggctgat caagcgcggg ctggcggcgt
     4201 ggtcgcagtg gcgccagcag gcgcgcgagg cacgaaccgc accccgggaa atcgtgtaat
     4261 aatttgttgt attattttat aatcatgcgt cattttataa catcaagaaa tggcttgcgg
     4321 tggcacggat atgggtctgt ctgcgccggc gtgagcggag cctgacaccc atgggcctgt
     4381 ggagcttcta cttcatcgcc aaggtcgcgc tgtatttcga ccatgcgatc gactttcatc
     4441 cgctgccgaa cctcgcct
//
LOCUS       DASZZC010000375         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_91787,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000375 DASZZC010000000
VERSION     DASZZC010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91787"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VHE37_08045"
     CDS             <1..277
                     /locus_tag="VHE37_08045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT35519.1"
                     /translation="DTVARLGGDEFGIVLSKVNDRAGALQQAERLTEQIRQPFMFEQK
                     ELPLDASIGCAIYPDDGADLDRLIERADQAMYAIKRDKKEKTREQPR"
     gene            complement(281..880)
                     /locus_tag="VHE37_08050"
     CDS             complement(281..880)
                     /locus_tag="VHE37_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVT35520.1"
                     /translation="MSQSPGAPRAQFTSIEHSTQKDWMIIASNLPAFARALPDRVMAH
                     LELLDGDFGGFPIDRLQHCLQTATLAHRDGRDEEYVCCALLHDIGDTLGTFNHPDIGA
                     AILKPFVREENHWMVQQHGIFQGYYFFHHLGMNRDLREQFRGHAHFERTAEFCAKYDG
                     PAFDGNTETAPLEFFEPMLRRLFSAPRNSIYSAAMQPPN"
     gene            complement(1061..>2758)
                     /locus_tag="VHE37_08055"
     CDS             complement(1061..>2758)
                     /locus_tag="VHE37_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HVT35521.1"
                     /translation="VYDFGRIFAGRVHLDLAGEPGRTLTLVHGEKLNDDGTVQVASGL
                     IDAQLQTHRYTFSSKDVEHWAPQFSYQGFRYVQLENAPGKPPLAALTAETVHSRVASA
                     GEFGSSNALLERIQAMARHALENNRHGNQTDTPTLEKNGWTGDAQASAQANVLNFDLA
                     RVWSKWLADFRDAQSAKGEIPEIVPSTPYYGYENTPGWGALWGPVPSWDAATFVLPWE
                     LYQAYGDRRILEQMYDTQKKLADYTGSYFNAKLAYSNPNNFLLGEYASVMPPGGIIAA
                     LRLAPNGPIDATASAYYYYMLDHLARSAGLLGKGDDAQVYRARAEAVRMAYNERYWDA
                     AAELYRMPGQKGEVKPFAETPNILAVAFGIAPAGHEAAIMKHVADDIATRGDHLGCGV
                     YAGRYVMTLLSDYGYADLAYRVATREDFPSWGYWLANGLQSMAEGWELSSRSWDHHYW
                     ASISSYFYQGLAGIRAGSPGYAQIVIAPRPPAGLDAAHAQIETLRGPVRSSWTRSAHE
                     FALEVSVPAGASAQVRVPGAGARLAQEPAGASFVRVEGDAAEYSVGAGDYRFSYTLK"
BASE COUNT          430 a          918 c          929 g          481 t
ORIGIN      
        1 cgacaccgtg gcgcgcctgg gcggcgacga attcggcatc gtgctgtcga aggtcaacga
       61 ccgggccggc gcgctgcagc aggccgagcg gctcaccgag cagatccgcc agccgttcat
      121 gttcgaacag aaggaactgc cgctggacgc gagcatcggc tgcgccatct accccgacga
      181 cggtgccgat ctcgaccgcc tgatcgagcg cgccgaccag gccatgtacg cgatcaagcg
      241 cgacaagaag gaaaagaccc gcgagcagcc gcgctagcgc tcagttcggc ggctgcatcg
      301 ccgcgctgta gatgctgttg cgcggcgcgg agaacagccg ccgcagcatt ggctcgaaga
      361 attccagcgg cgcggtttcc gtgttgccgt cgaacgccgg cccgtcgtac ttcgcgcaga
      421 actcggcggt gcgctcgaaa tgcgcatggc cgcggaactg ctcgcgcagg tcgcggttca
      481 tgcccaggtg atgaaagaag tagtagccct ggaagatgcc gtgctgctgc accatccagt
      541 ggttctcctc gcgcacgaac ggcttgagga tggcggcgcc gatgtccggg tggttgaagg
      601 tgcccagcgt gtcgccgatg tcgtgcagca gcgcgcagca cacgtattcc tcgtcgcgtc
      661 cgtccctgtg cgccagcgtc gccgtctgca ggcagtgctg caggcggtcg atgggaaagc
      721 cgccgaagtc gccgtcgagc agctccaggt gcgccatcac gcggtcgggc aaggcgcgtg
      781 caaacgcagg caggttggac gcgatgatca tccagtcctt ctgcgtgctg tgctcgatgc
      841 tggtgaactg cgcgcgcggc gcgccgggtg actggctcat gcggatctcg ctggattgcc
      901 ggggacgcgc cagcatattc ctgtcggcct gctcggcgca gggcatggcg gcgaccgcca
      961 gaaaccatcc accgcacccg ctcctatcat gttattaaat atcttacgtt tttttattta
     1021 ttaaattatt tataacacac caaataccgg tgcggtggag tcatttcagc gtgtaggaga
     1081 agcggtaatc gccggcgccg acgctgtact ccgctgcatc gccttcgacg cgcacaaagc
     1141 tcgcgcctgc cggttcctgc gccaagcgcg cacctgcgcc cggcacgcgc acctgcgcgc
     1201 tcgcgcccgc gggcacgctg acctcgagcg cgaattcgtg cgcgctgcgc gtccacgagg
     1261 agcgcaccgg cccgcgcagg gtttcgatct gcgcgtgcgc ggcatcgagg cccgccggcg
     1321 gacgcggcgc gatgacgatc tgcgcgtaac ccggcgaacc ggcgcggatg ccggccaggc
     1381 cctggtagaa atacgaactg atcgacgccc agtagtggtg atcccagctg cgcgacgaca
     1441 gctcccagcc ttccgccatg ctctgcagac cattcgccag ccagtaaccc cagctgggga
     1501 aatcctcgcg cgtggccacg cggtaggcga gatcggcgta gccgtagtcg gacagcagcg
     1561 tcatcacgta gcggccggcg tagacaccgc agcccaggtg atcgccgcgc gtggcgatgt
     1621 cgtcggccac gtgtttcatg atcgccgcct cgtgcccggc gggcgcgatg ccgaacgcca
     1681 ccgcgagaat gttcggcgtt tcggcgaaag gcttcacctc gcctttctgg ccgggcatgc
     1741 ggtagagctc agcggcggcg tcccagtagc gctcgttgta ggccatgcgc acagcctcgg
     1801 cgcgcgcgcg gtagacctgc gcgtcgtcgc ccttgcccag cagcccggcg ctgcgcgcga
     1861 ggtgatcgag catgtagtag tagtaggcgc tggcggtcgc atcgatcggg ccattgggcg
     1921 ccagccgcag cgcggcgatg atgccgcccg gcggcatcac gctggcgtat tcgccgagca
     1981 ggaagttgtt cgggttgctg tacgccagct tcgcgttgaa atagctgccg gtgtagtcgg
     2041 ccagcttctt ctgcgtgtcg tacatctgtt ccaggatgcg gcggtcgccg tacgcctggt
     2101 acagctccca cggcagcacg aaagtggcgg cgtcccagct cggcaccgga ccccacagcg
     2161 cgccccagcc tggcgtgttc tcgtagccgt agtacggcgt cgacggcacg atctccggga
     2221 tctcgccctt ggcgctctgc gcatcgcgga aatcggccag ccacttgctc cacacgcgcg
     2281 cgagatcgaa gttgaggacg ttggcctgcg cgctggcctg cgcgtcgccg gtccagccgt
     2341 tcttctccag cgtgggtgtg tcggtctggt tgccgtggcg gttgttctcc agcgcgtggc
     2401 gcgccatcgc ctggatgcgc tcgagcagcg cgttgctgct gccgaattcg ccggccgacg
     2461 ccacccggct gtgcacggtc tcggcggtga gcgccgccag cggcggcttg ccgggcgcat
     2521 tttccagctg cacgtagcgg aagccctggt agctgaactg cggcgcccag tgctccacat
     2581 ccttgctgct gaaagtgtag cggtgcgtct gcagctgcgc gtcgatcagc ccgctggcga
     2641 cctgcacggt gccgtcgtcg ttgagcttct cgccatgcac cagcgtcagc gtgcggccgg
     2701 gctcgccggc caggtccagg tgcacgcgcc cggcgaagat gcggccgaag tcgtagac
//
LOCUS       DASZZC010000376         5493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_285871,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000376 DASZZC010000000
VERSION     DASZZC010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285871"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VHE37_08060"
     CDS             <1..392
                     /locus_tag="VHE37_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35522.1"
                     /translation="RARDWFRPRRFAEFPNPHLRTNGFMAAREVLLQLRVPPIRNKDD
                     AHRFESGVHGFTAQLKGLGLRSLLVGADGKAYEPAEWPESRTFRLGDQDNLLMADNQT
                     RTYLAADADTRRKLSNLAWGARAPSTK"
     gene            389..1324
                     /locus_tag="VHE37_08065"
     CDS             389..1324
                     /locus_tag="VHE37_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVT35523.1"
                     /translation="MNKQKTALPMTEAPCIHCGHTATLLFQARDLNHHVDTADFNYFR
                     CPGCRLIFLQPVPDNLGAYYDVAYPPYSIPGNAAELDRAAQRVRWRVDFLKRYAPAGG
                     RLLEIGPSYGAFAYAAAQAGFRVDAIEMDARCCDFINKHVAGARAIHTSNVVDGLKHL
                     DGGYVAIVLWHNLEHLLAPMTVLRNLVEQLAAGGVLLVATPNPDSLQFRMFGRHWVHL
                     DAPRHVALIPPGLVDATLRPHGLVRECYTTTDPDGLALSRMSSDISLAYALGLPVENG
                     LRKWLSRILRWVLRPAENSLQRGSAYTVVYRRPAR"
     gene            1333..2334
                     /locus_tag="VHE37_08070"
     CDS             1333..2334
                     /locus_tag="VHE37_08070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT35524.1"
                     /translation="MDCRFSVVLPVYNGASYVGTAVRSILDQTFADFELVVLDNCSDD
                     GTAAIVAAAAGGDPRVRLLGSDSVRSMEQNWDRARQLCAGSEIRGEFLTLIGHDDLLL
                     PGFLQTISDLIRACPQARLYQTHFRIIDGSGQVRRRCRPIPLRESAAGYFRARCWGMR
                     DSFGTGQVFRRQDYLACGGIPQYPRLLYADDMLWLKLLGEHGEMACSEQVCFEYRLHD
                     NNTSSIATTAAYQASLAALRMFVEEIDAQLPWLLDGEMNRAGLKLRLTSALREIEKGS
                     YRLGFDPALVNDDWTALAARHDRLGQWLNDPSDVPLTLRPRRLKRALHALRRWRRGR"
     gene            complement(2272..3489)
                     /locus_tag="VHE37_08075"
     CDS             complement(2272..3489)
                     /locus_tag="VHE37_08075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVT35525.1"
                     /translation="MFVVLACIRAGIVQEPLVFKPLAQPLRPPRRILVICTRRIGDVL
                     LSTALLRSLKHAWPQARIDVLTNAGSAAVLEGNPDIERLLLLPDRPGVGEFLRCAARI
                     FRRYSLALSTLPNDRQHVLAFLSAPVRVNAVPPPVETSARWKRWLSDGWAMVNLSRHH
                     AVTQYLLLADCLRIPRVAEVVPPRAAAPRLPLANAYAVVHPRPMYRYKEWPVQCWVAL
                     LRWLAGQGLQVVLTGGPGAEETAYVQGIAAAAALPDAALLNTAGQLRFAELAELIRGA
                     RLFVGPDTSVTHLAAATGAPTVALFGPSPPVSWGPWPNQFKLVVDSPWVMTSPLQHQG
                     NVWIVQGITPCVPCLREGCDRHLQSRADCLDQLPAARVIAVAAQALTGTGASAAAAPA
                     PEGVQGALQPARP"
     gene            3533..4630
                     /locus_tag="VHE37_08080"
     CDS             3533..4630
                     /locus_tag="VHE37_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HVT35526.1"
                     /translation="MRNANRMPPNFAIEATCLLDRNQTGISRYTRNLIAQLAQLADGG
                     REFVVTQAYRASRWRRRMQLSDSGPLRRQVWQRGWWPPVKPYALVHCPDHRLPHWRIP
                     KIVTIHDVYAALGINFEGANRDKQVAIYADFARRADRIIFDSEHSRQDFMRLFPYDIQ
                     RTRVIHLGVDASFHPRPPEALAATRAKYGLDRPYLLFLGVNNPNKNLLRLVEAYSRCA
                     ARRDLQLVLAGPLRPQPEAQLRQRLRELDIESTTRMLGYLNAADVPLLYAGAAALMLP
                     SIYEGFGLPILEAMASAIPVLASTAASCPEVAAGHAVLVDPASVDDIASGIDRVLTLT
                     AEQIEAARRYAVGKTWRATALQTLEVYRDVS"
     gene            4620..>5493
                     /locus_tag="VHE37_08085"
     CDS             4620..>5493
                     /locus_tag="VHE37_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT35527.1"
                     /translation="MSAEPAAARWLLLAHAFNMDGVASSQTVTDKLPYLAQAGVRIVA
                     VTAPTGRHDAQLEHHQVFSPLPSGLRYEWRHWLKRNAGGRARIYSRIALALLLPFYLL
                     EKILLPFGSHWSWWISAYRRGARIIERERPALIYSSGGANSAHYAAYRLAQRYQLPWL
                     AEVHDPMIHDGWQKGPFQHWWAAHVEKLICRRADAAIWFTPTALARARARNPQLGGRG
                     HLMYPGANPPPHAKIPYLRGEKFVLAHFGSLADNRNLVRVLEAMAQMLQAHPQWREKF
                     LLRAYGGGWDRNTRA"
BASE COUNT          914 a         1915 c         1785 g          879 t
ORIGIN      
        1 tgcgcgcacg cgactggttc cggccgcggc gctttgccga attccccaac ccgcacctgc
       61 gcaccaacgg cttcatggct gcgcgtgaag tgctgctgca gctgcgcgtt ccgccgatac
      121 gcaacaagga cgatgcgcac cgcttcgaga gcggcgtcca tggattcacc gcccagctca
      181 agggacttgg cctgcgtagc ctgctggtcg gcgccgacgg caaagcctac gagccggcag
      241 aatggccgga gagccggact ttccgcctgg gcgaccagga caacctgctg atggccgata
      301 atcaaacccg cacctacctt gccgccgatg ccgacacgcg gcgcaaactg tcgaacctgg
      361 cctggggagc gcgagctccc agcacaaaat gaacaagcag aagaccgcgc tgccgatgac
      421 cgaggcgccg tgcatccatt gcgggcatac cgcgacgctg ctgttccagg cgcgcgacct
      481 caaccatcat gtcgacaccg ccgacttcaa ctatttccgc tgccctggct gcaggctcat
      541 cttcctgcaa ccggtgccgg acaacctcgg cgcctattac gatgtcgcct atccgcccta
      601 cagcattccg ggcaacgccg cggaactgga ccgggcagcg cagcgcgtgc gctggcgggt
      661 ggacttcctc aagcgctacg cgcccgcggg cggacggctg ctggagattg ggccttcata
      721 cggcgccttc gcatatgccg ccgcgcaggc aggtttccgc gtcgatgcga tagaaatgga
      781 tgcgcgctgc tgcgacttca tcaacaagca cgtcgccggt gccagggcca tccacaccag
      841 caacgtggtt gacggcctga agcaccttga tggtggctat gtcgcgatcg tgctctggca
      901 caacctcgag cacctgcttg cgccgatgac cgtgttgaga aacctggtcg agcagttggc
      961 tgcaggcggc gtactgctgg ttgccacgcc caaccctgac tcgctgcagt tccgcatgtt
     1021 cggccggcac tgggtgcacc tggacgcacc gcggcatgtg gcgctgattc caccgggcct
     1081 ggtcgacgcc acgctgcggc cgcatggcct ggtgcgggag tgctacacca ctactgaccc
     1141 cgatggcctg gcactcagcc gcatgagttc cgacatctcg ctggcctatg cgctgggcct
     1201 gcccgtggag aacggcctgc gcaaatggct ctcgcgcatc ctgcgctggg tgttacggcc
     1261 cgcggaaaac agcctccagc gaggctcggc ctacaccgtg gtctatcgca ggccggcgcg
     1321 ttgagatcgc cgatggattg ccgtttctcc gtcgtgctgc cggtatacaa cggcgcgtct
     1381 tacgtgggaa cagcggtacg cagcatcctc gatcagacct tcgccgattt tgaactggtg
     1441 gtgctcgaca actgcagcga tgacggcacc gccgccatag tggccgcggc tgccggcgga
     1501 gatccgcgcg tgcgcctgct cggctcggac agtgtgcggt cgatggaaca gaactgggac
     1561 cgtgcccgcc agctgtgcgc gggcagcgaa attcgcggcg agttccttac cctgattggt
     1621 catgacgacc tgctgctgcc cgggttcctg cagaccattt ccgacctgat ccgggcctgt
     1681 ccgcaggcac ggctctacca gacccatttc cgcatcatcg acggcagcgg ccaggtgcgc
     1741 cgccgttgcc ggccgattcc actgcgcgag tcggccgccg ggtacttccg cgcccgctgc
     1801 tggggcatgc gtgactcgtt cggcacgggc caggtgttcc gccggcagga ttacctggca
     1861 tgcggcggta tcccgcaata tccgcgcctg ctgtatgccg acgacatgct atggctgaag
     1921 ctgctcggcg agcacggcga aatggcctgc tcggaacagg tgtgctttga ataccgcctg
     1981 cacgacaaca acacttcgag cattgccacg accgctgcct accaggcctc gctagccgca
     2041 ctgcgcatgt tcgtcgaaga aatcgacgcg caactgccgt ggctgctgga tggcgagatg
     2101 aaccgcgcgg gactcaagct gcggctgact tcggccttgc gcgagatcga aaaaggcagt
     2161 taccgcctcg gcttcgatcc tgccttggtg aacgacgact ggacggcact ggccgcgcgc
     2221 cacgaccggc tcggccagtg gctgaatgat ccgtcggacg tcccgctgac gctacggccg
     2281 cgccggctga agcgcgccct gcacgccctc aggcgctggc gccgcggccg ctgacgcgcc
     2341 ggttccggtc aaggcctgcg cggcgacggc gatcacacgc gcggccggca actggtccag
     2401 gcaatcggca cggctttgca ggtgacgatc gcagccttcc cgcaggcacg gcacacatgg
     2461 cgtgatgccc tgcacgatcc acacgttgcc ttgatgctgc agcggcgagg tcatcaccca
     2521 cgggctgtcg accacaagct tgaactggtt cggccaaggc ccccagctga ccggcggcga
     2581 cggcccgaac aaggccacgg tcggcgcccc ggttgcggcc gcaaggtggg tgaccgaggt
     2641 atccggcccg acaaaaaggc gcgcaccgcg gatcagttcg gccagttcgg caaagcgcag
     2701 ctgcccggcg gtgttgagca gcgccgcatc cggcaatgct gccgccgccg cgatgccctg
     2761 cacgtaggcc gtctcttccg cacccggacc gccggtgagc accacctgca gcccctggcc
     2821 ggcaagccag cgcagcagcg cgacccagca ctgcacgggc cactccttgt agcggtacat
     2881 cggccgcgga tgcaccactg cataggcatt ggcgagcggc aaccgcggcg ctgcggcgcg
     2941 cggcggcacc acctcggcaa cccggggaat gcgcaggcag tcggccagca acaggtactg
     3001 ggtcaccgcg tggtggcggc tcagattgac catggcccag ccgtcggaga gccagcgctt
     3061 ccagcgcgcg ctggtctcga ccggcggtgg caccgcgttc acccgcaccg gtgcggagag
     3121 aaaagccagc acgtgctggc ggtcgttcgg caacgtggag agtgcgagtg aatagcggcg
     3181 gaaaatgcga gcagcgcagc gcaggaattc gcctacgccc ggccggtccg gcaacagcag
     3241 cagccgctcg atgtccgggt tcccttcgag caccgcggcg gagcctgcat tggtcagcac
     3301 gtcgatccgc gcctgcggcc acgcgtgctt tagcgagcgc agcagcgccg tgctgagcag
     3361 cacgtcgccg atgcggcggg tgcagatcac caggatacgg cggggcggac gcagcggctg
     3421 ggccaggggt ttgaacacga gtggctcttg aacaatgccg gcgcggatac acgccaggac
     3481 aacgaacatt ctatagttcc cgcttgacca tcccacgact gatacggcgc ccatgcgcaa
     3541 tgcgaaccgg atgccgccca acttcgccat cgaggcaacc tgcctgctgg accgcaacca
     3601 gaccggcatc tcgcgctaca cccgcaacct gatcgcgcaa ctggcgcagc tggccgacgg
     3661 cggccgtgag ttcgtggtga cgcaggccta ccgcgcctcg cgctggcgcc gccgcatgca
     3721 actgtccgac agcggtccgt tgcggcgcca ggtgtggcag cgcggctggt ggccgccggt
     3781 gaagccctac gcgctggtgc attgcccgga ccatcgcctg ccgcactggc gcatccccaa
     3841 gatcgtcacc atccacgacg tgtatgccgc gctgggcatc aacttcgaag gggccaaccg
     3901 cgacaagcag gtggccatct atgccgactt tgcccggcgc gccgaccgca tcatcttcga
     3961 cagcgaacac tcgcgccagg atttcatgcg cctgttcccg tacgacatcc agcgcacccg
     4021 cgtgatccac ctgggcgtgg atgccagctt ccacccgcgg ccgccggaag cgttggcggc
     4081 gacgcgcgcg aaatacggcc tcgatcgacc ctacctgctg ttcctgggcg tcaacaatcc
     4141 gaacaagaac ctgctgcggc tggtggaggc gtacagccgc tgtgctgcgc gccgcgatct
     4201 gcagttggta cttgccgggc cgctgcggcc gcaacccgag gcccagctgc gccagcgcct
     4261 gcgcgaactg gatatcgagt ccaccacgcg catgctcggt tacctcaacg ccgccgatgt
     4321 tccgctgctg tacgctggcg ccgcggcact gatgctgcct tcgatctatg agggcttcgg
     4381 cctgccgatc ctggaagcca tggcgtcggc cataccggtg ctggccagca ccgctgcgag
     4441 ttgcccggaa gttgccgcgg gccatgcggt gctggtggac cccgcctccg tcgacgacat
     4501 cgccagcggc attgaccgcg tgctgacgct gaccgcggag cagatcgagg ccgcccgccg
     4561 ctatgccgtg ggcaagacct ggcgcgccac cgcgctccag accctggagg tgtatcgcga
     4621 tgtcagctga gccggctgcg gcccgctggc tgctgctggc gcacgccttc aacatggacg
     4681 gcgtggcctc cagccagacg gtgacggaca agctgccgta cctggcccag gccggcgtgc
     4741 gcatcgtcgc ggtgaccgcg cccaccgggc gtcatgacgc gcagctggag caccaccagg
     4801 tattttcgcc gctgccgtcc gggctgcgct acgaatggcg ccactggctc aagcgcaacg
     4861 ccggagggcg cgccaggatt tactcgcgca tcgcgctggc gctgttgctg ccgttctacc
     4921 tgctggaaaa aatcctgctg cccttcggca gccactggtc gtggtggatc agcgcctatc
     4981 gccgcggcgc gcgcatcatc gagcgcgagc gtccggcgct gatctattcc tccggcggcg
     5041 ccaacagcgc gcactacgcc gcgtaccggc tggcgcagcg ctaccagctg ccgtggctgg
     5101 ccgaagtgca cgacccgatg atccacgatg gctggcagaa aggcccgttc cagcactggt
     5161 gggcggcgca tgtcgagaag ctgatctgcc ggcgcgccga cgcggccatc tggttcacgc
     5221 ccaccgcgct ggcccgcgcg cgcgcgcgca atccgcagct gggcggtcgc gggcacctga
     5281 tgtaccccgg cgccaatccg ccaccgcacg ccaagattcc gtatttgcgc ggcgaaaaat
     5341 tcgtgttggc gcatttcggc tcgctggccg acaaccgcaa cctggtgcgc gtgctcgaag
     5401 cgatggcgca gatgctgcag gcgcatccgc agtggcgcga aaaattcctg ctgcgcgcct
     5461 atggcggcgg ctgggaccgc aacacgcggg ccg
//
LOCUS       DASZZC010000377        12605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1249521,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000377 DASZZC010000000
VERSION     DASZZC010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249521"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VHE37_08090"
     CDS             complement(<1..878)
                     /locus_tag="VHE37_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT35528.1"
                     /translation="MRLRRLALLGVLVLAACAPKEILYPLAGQDVPRLHDFPAGFEPP
                     VSAETAREIGGFGGEVDHLSHTPVIFVHGNTVSASFWLPAREHFAAAGYNIGELWAPS
                     YGWSSVRSFDSDDLSVPTLDAFVTELQRYLKIRTGRDVQQFDIVAHSLGVTLVRQWML
                     QTNSFHRVRNFIGVCGANHGVWTARPDARGINRVSAFELMPGSPWLAQLNRAGETPGP
                     VRYMTLYDGIGWGDELFPKPYQESSALDGARNLAFNREHGTHYTHLELPRQPDTLDAM
                     LDFLRSAHEPLPGAVPP"
     gene            complement(875..2077)
                     /locus_tag="VHE37_08095"
     CDS             complement(875..2077)
                     /locus_tag="VHE37_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HVT35529.1"
                     /translation="MRPKNAARKLLCAAALALVAASAQASILFTPHLSEYAPLAEGSY
                     ADHTLVYTSIRKVYDANGQVIPLGAPFIPGGDSVDALLLLARYLWIGNQFADTRVPLL
                     STHKQIFRVIATAGWQQASGGAVDRSRLFGLTSGGSGIGDVYVLGGIYTHEHRWGPLQ
                     WNGLFSNTLKLPVGEYDTKALLNNGTHYWTTIPQIAAHAELYNRLIVDGTFAWQFNGR
                     NDSPAYGGMTPTDPADVRNFEVNAAWKFSEKWFVDFGFFRRQSVGPNKFGKVTATFTS
                     PQPATTACAALGIPAAQCSLTNDFRLVPVPGERQDNGIYQNLLSSSIYYVYRTSSVVD
                     FRVSRPISGRGSQFPMDYDVALYSDPTQTPIPGTRQHVVLNGVQEAAAIPASPLFELR
                     FVYLFWAP"
     gene            complement(2172..3341)
                     /locus_tag="VHE37_08100"
     CDS             complement(2172..3341)
                     /locus_tag="VHE37_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HVT35530.1"
                     /translation="MKILARVALLAACFTGARAQASAEDWEIGRYLSKYPAMYVDAGL
                     WHDARDRTFSASGARQDGVAPTYGGNSRFPVTEGDVTLEWHFPFFEPEALPFISSRLW
                     TARATLGYARTHSEGAMADYADAHGLVEKADGITDIALEFGPVLWGSDNWRERKDHPL
                     SVLLLGLATLPVGAREAESPNNLGSNKFSFGARLGAHARPLPRVLIDAGLSFQTYLAD
                     DEPAFGGQEPHRRGDDRSFDLTTALRVYRGIYAAASLRLRDGAANEYEKLQYTATTPA
                     AGVGMETFPDPAPAHDGGTRDARLGLALHAFVAPRVQIGVHFIHPLSGRSGEFQQHYL
                     QQTQNCAALGGCAPADNGYVPVDGLGSARVYASNVWMLTLNWNLQQGDPWLGDEP"
     gene            complement(3338..4804)
                     /locus_tag="VHE37_08105"
     CDS             complement(3338..4804)
                     /locus_tag="VHE37_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT35531.1"
                     /translation="MSLDLIAAHDPARPALWWDGRWYRYGELGLRARRLAAALASIGI
                     ARGDRVAIYAHNHLAHIELVLAAPLLGFIYTPYNFRLSAGEQQALTRYLKPRLMLADA
                     AHLPFARTLGVEMLALDQYESWIDTARAALPPVELDERDAHMILLTGGSTGLPKGALI
                     SYRQVLANCTATAAGWDLWPEDCAIQATPCFHAALNVFTTPLLKLGGRVVLMRDFEPG
                     AYLRLAAQHGASVLFMVPTMYRMLTEHADFPAADLSQVRWAISGGAPCPPTVRAPFAA
                     RGIRFRQGYGMTECGVNCFAIGLDEAEKNPDAVGRPLPQLQAAVRRADGSACAADEIG
                     ELTLSGPQVCSGYFEREAQWRKVYRDGWLWTGDLARVGADGLYRIVGRSKEMYISGGE
                     NVYPAEVEAALAQCAGVAECAVIGIADEKWGEVGLAAVVLKPGHAADEAALRAELKTR
                     LAGYKVPRAYFFLPALPKSGAGKILKPDIRRQYEQPRA"
     gene            complement(4801..5793)
                     /locus_tag="VHE37_08110"
     CDS             complement(4801..5793)
                     /locus_tag="VHE37_08110"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HVT35532.1"
                     /translation="MIVRGLGIYIPEPRHSAADIAAASGLPEAVVRDKLGITAKPVPG
                     PEDHTNAMGVRAARAALADARIDAREVDALICITEEHKEYPVWTAGIQAAHELGAVNA
                     YAFDLGQKCGTAVLALKLARDMIKADGVSTVLIAGGYRNGDLIDYADPATRFLNNLAA
                     GGGAAVVTRGGSGFEILGSAFRTDGSFSLDVIVPVGGTVEPLTADNVEQFRFQVRDSA
                     GMKARLERVSLENFVGVIEQACARSGIRPREAAYVAMLHMKRSAHQQILERVGVPPER
                     SIYLSDYGHLGQIDPLLSLQLAREQGRLRKGDIAVLCAAGIGYVWNAICVRMTA"
     gene            complement(5793..6434)
                     /locus_tag="VHE37_08115"
     CDS             complement(5793..6434)
                     /locus_tag="VHE37_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT35533.1"
                     /translation="MSYQAASVPMSSTHAPIVPATARGEATRRKLLDAAEKEFGGKGF
                     HAASVSSITTRADVGQGTFYLYFHSKEEIFVTLVRDIGHALRKHMAEAVGKATPRMAA
                     ERAGLEAFLEFAQQHPGLYRIVQECQFVDEGAFREYYQRLASGYTQAIEAATARSELS
                     PGNAELRAWSIMGIGHFIGMRHCLWEGKAPDAAQMDDLMDFVARGMAPRKHES"
     gene            complement(6645..8120)
                     /locus_tag="VHE37_08120"
     CDS             complement(6645..8120)
                     /locus_tag="VHE37_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HVT35534.1"
                     /translation="MLMRTHVARKLVAARRRELDQAADYATWKEIALELDRLEGADAW
                     KQDDTSDDFDHLLIKERLAQMRALRTRGDVRQLVFNLYEGLHGNLGNISNAALYAQAR
                     VGTKQLIEQYVDEVARCLDYICAGDFPDFSHDEKILFFKRTGTVYGRSALMLSGGATL
                     GMFHLGVIKALQEQGLLPRVLSGSSAGSIIAGVAGTRDDAALKRVFEPGGLNLQAFQS
                     VTLRRAIKGRAVMDAGALEKCLADNIGGETFVEAFERTRRIVGVTISPAEPHQQGRLM
                     NYLTSPHVLLSKAILASCAVPGVFPPVMLEGRDYLGRVTPYMPSKRWVDGTLSADLPM
                     LRTARLHNVNHYIVSQTNPHIVPFMSDKLPSTGLAPLARELVAHGGRGALKLARKHLD
                     PYGGGRVINKVDNIVKQRYSGDINIFPRQTPRQLLNMFANPSAEEIQAYIREGERATW
                     PKLDRIRTATRISRAFEDCTALLKQRAVDSRKKPRWLRVVS"
     gene            8345..10912
                     /gene="mprF"
                     /locus_tag="VHE37_08125"
     CDS             8345..10912
                     /gene="mprF"
                     /locus_tag="VHE37_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HVT35535.1"
                     /translation="MESAAPIVPAETTWSRLRSWLPTLIALALIAMVLLAIRSLLREV
                     SYDEVADAVLTTRRSAIAWSVLATVASYISLLGYDWSGARYAGIRLPRGTLALASFVG
                     YALGNSVGLGALTGGAVRFRIYSAAGIEAGRIGKLVMFIVSAFGAGVTVVAAASLMWA
                     ATHVGHVAHLSPLLLRVIAGSVLALAAGFIVLCFARREVLLLGRWQLQLPTGGVALAQ
                     LAVSVADIIAAAAALWFLLPGARESFPQFVAFYSIALAAGVISHVPGGLGVFEGVLLL
                     AYGHRAPLDALAGALLLYRAIYFIGPLFLAMLVLAFYELRASVAGVGRAAAQLSPMLL
                     SVWTLIVGIMLLVSGVTPATGNATELLSLHVPLQIVEASHFIGSIAGLGLLFVARGLL
                     YRLDAAWWAALVLTIVAFWLALPKGIALTEMAVLAFLAFNLIVGRREFDRRASLFSQV
                     FSRGWMLMVGAVLVGVGWVMFFVYQDIDYTHELWWQFAFDAHAPRSLRLLLAAVVSTL
                     GVAMWQWFRPYSGNAGGCSPADLEHARGVLREQPRADACLALTGDKSLLFSRSGKAFL
                     MYGKRGRTWVSLYDPVGAQQDLPELVWSFMELAAAHGGRAVFYQVRPQNLSLYVDTGL
                     RPFKLGEDARVALASFTTKGSANAHLRQAINKGEREGLSLEIVPAEGVAAQLPELARI
                     SDAWLETHNTREKHFSLGAFDPNYLAALPVAVVRRAGAPIAFANILCTALKDEARVDL
                     MRYAPGAPNGTMDYLFVKLMLHFQAEGYAHFGLGMAPFSGLVRHPLAPRWHRLGSLLF
                     GLGEHFYNFQGLRSFKEKFHPQWEPRYLCSPGGSAPLFALADIASLTSGGLKGVISK"
     gene            10909..12282
                     /locus_tag="VHE37_08130"
     CDS             10909..12282
                     /locus_tag="VHE37_08130"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HVT35536.1"
                     /translation="MKRWLWMLLLCAGAAQAQEQVAHGRFDDVMLYRPAGEPQSFVLF
                     LSDADGGDAPTTRMMRTLVARGALVAGINTRDFFAAMEADGAPCQYADGDLENLSHFL
                     QAYAQVPGYLPPLLAGKGVGASFAYAVLAQAPKGMFAGALALGFTRELPLHQKTLCEL
                     PTAQARADGSGLDLLPAARLSGPLRVLHGEADDVAPLAGIASFMALVPQAQTIPLPNV
                     GHGFGNWRDWQALLGVAYEELVAPIRPAMRAPAGDVADLPVIELPVQQSGDSFALIIS
                     GDGGWAGLDKNLASALLARGIPVVGLDSLRYFWSPRTPEGVAHDVERLIRHYSARWQK
                     KRVLLIGYSQGGDVLPFAYNRLSPAVRAQVALAVPISISDRAAFEFHMTNWVGGKGDV
                     PTMPEMDKLAGNSTLFCVYGADDKESLCSQLDPKRFRVKSLPGAHHFNGDYAKLAATV
                     LEALPKD"
     gene            12284..>12605
                     /locus_tag="VHE37_08135"
     CDS             12284..>12605
                     /locus_tag="VHE37_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HVT35537.1"
                     /translation="MEGLVIPALWALAVLLVLGGIVGTVLPVLPGAPMMFGGLWLAAW
                     LDHYAHAGLITLLLLALLTFISMAVDWIAGALGAKKVGASPRAISGAAAGSLLGGMFL
                     FPWGL"
BASE COUNT         1855 a         4224 c         4398 g         2128 t
ORIGIN      
        1 ggcggcaccg cgccgggcag cggctcgtgc gcactgcgca ggaagtcgag catcgcatcc
       61 agcgtgtcgg gctggcgcgg cagttccagg tgcgtgtagt gcgtgccgtg ctcgcggttg
      121 aaggcgagat tgcgcgcgcc atcgagtgcc gacgactcct ggtagggctt ggggaacaac
      181 tcgtcgcccc atccgatgcc gtcgtacagc gtcatgtagc gtaccggccc gggcgtctcg
      241 ccggcgcggt tgagctgcgc cagccacggg ctgcccggca tcagctcgaa ggccgacacg
      301 cggttgatgc cgcgtgcatc gggccgcgcg gtccacacgc catggttggc gccgcacacg
      361 ccgatgaagt tgcgcacgcg gtggaagctg ttggtctgca gcatccactg gcgcaccagc
      421 gtcacgccca gcgaatgggc cacgatgtcg aactgctgca cgtcgcgacc ggtgcggatt
      481 ttcagatagc gctgcagctc ggtgacgaac gcatccagcg tgggcaccga caggtcgtcg
      541 gaatcgaacg aacgcacgct gctccagccg tagctcggtg cccacagctc gccgatgttg
      601 taaccggcgg cggcgaagtg ctcgcgcgcg ggcagccaga aactggcgct caccgtgttg
      661 ccgtggacga agatcaccgg cgtgtggctg agatgatcga cctcgccgcc gaagccgccg
      721 atctcgcgcg cggtttccgc cgacaccggc ggctcgaagc cggcggggaa atcgtgcagc
      781 cggggcacgt cctggcctgc cagcgggtaa agaatttctt ttggcgcgca ggcggccagc
      841 accaacacgc cgagcagcgc gagccggcgc agcctcatgg cgcccagaac aaatagacga
      901 agcgcagctc aaacagcggc gacgccggga tcgccgccgc ctcctgcacg ccgttgagca
      961 ccacgtgctg gcgcgtgccg ggaatgggcg tctgcgtggg gtcgctgtac agcgctacgt
     1021 cgtaatccat ggggaactgg ctgccgcggc cgctgatggg ccgcgacacg cggaagtcca
     1081 ccaccgacga ggtgcggtat acgtagtaga tgctgctcga cagcaggttc tggtagatgc
     1141 cgttgtcctg ccgctcgccc ggcaccggca ccaggcggaa atcattggtg agcgagcatt
     1201 gcgccgcggg aatgccgagc gcggcgcagg cggtggtggc cggctgcggc gaggtgaagg
     1261 tcgcggtgac cttgccgaac ttgttcggcc ccaccgactg gcggcggaag aaaccgaaat
     1321 cgacgaacca tttttcggaa aacttccacg ccgcgttcac ttcgaagttg cgcacgtcgg
     1381 ccgggtcggt cggcgtcatg ccgccgtacg cgggagagtc attcctgccg ttgaactgcc
     1441 aggcgaaggt gccgtcgaca atcagccggt tgtacagctc ggcgtgcgcc gcgatctgcg
     1501 gaatggtggt ccagtaatgc gtgccgttgt tgagcagcgc cttggtgtcg tactcgccca
     1561 ccggcagctt cagcgtgttc gagaacaggc cgttccactg cagcggaccc cagcggtgct
     1621 cgtgggtgta gatgccgccg agcacgtaga cgtcgccgat gccgctgccg cccgaggtga
     1681 gcccgaacag ccgcgagcga tccaccgcgc cgccggaagc ctgctgccag ccagcggtgg
     1741 cgatcacgcg gaagatctgc ttgtgcgtgg acagcagcgg cacgcgcgtg tcggcgaact
     1801 ggttgccgat ccacaggtag cgcgccagca gcagcagcgc atccacgctg tcgccgccgg
     1861 ggatgaacgg cgcgccgagc gggatcacct ggccgttggc gtcatacacc ttgcggatgc
     1921 tggtgtagac cagcgtgtga tccgcgtaag acccttcggc cagcggcgca tattccgaca
     1981 ggtgcggcgt gaacaggatg ctggcctgcg cgctggcggc gaccagcgcc agcgcggctg
     2041 cgcacaacag cttgcgcgcg gcgttttttg gcctcaccgc actctcgatt ttgatgttat
     2101 aaaaactatc ataattgtaa aataacttaa taattaataa catcacaaat ggcagtgcgg
     2161 tgcaggctct ttcatggctc gtcgccgagc cagggatcgc cctgctgcag gttccagttc
     2221 agcgtgagca tccacacgtt gctcgcgtac acgcgcgccg agccaaggcc gtccaccggc
     2281 acgtagccgt tgtcggccgg cgcgcagccg cccagcgccg cgcagttctg cgtctgctgc
     2341 aggtagtgct gctggaattc tccactgcgc ccggacaatg gatgaatgaa gtgcacgccg
     2401 atctgcacgc gcggcgccac gaacgcgtgc agcgccaggc ccaggcgcgc gtcgcgcgta
     2461 ccgccgtcgt gcgcgggcgc aggatcggga aacgtctcca tgccgacgcc cgcggctggc
     2521 gtcgtcgcgg tgtactgcag cttctcgtac tcgttggcgg cgccgtcgcg caggcgcagc
     2581 gacgcggcgg cgtagatgcc gcgatacacg cgcagcgcgg tggtcaggtc gaagctgcgg
     2641 tcatcgccgc gccggtgcgg ctcctgcccg ccgaaggccg gctcatcgtc ggccagatag
     2701 gtctggaagc tgaggccggc gtcgatcagc acgcgcggca gcggccgcgc atgcgcgccc
     2761 agccgagcgc cgaaggagaa cttgttgctg ccgaggttgt tgggcgattc cgcctcgcgc
     2821 gcgcccaccg gcagcgtggc caggccgagc agcagcaccg acagcggatg atccttgcgc
     2881 tcgcgccagt tgtccgaacc ccacagcacc ggcccgaatt ccagcgcgat gtcggtgatg
     2941 ccatcggcct tctcgaccag cccatgcgca tccgcgtaat ccgccatcgc gccttcgctg
     3001 tgcgtgcgcg cgtagccgag cgtggcgcgc gcggtccaca ggcgcgagga gatgaacggc
     3061 aatgcttcag gctcaaagaa cgggaaatgc cattccaggg tgacgtcgcc ctcggtgacg
     3121 ggaaagcgcg agttgccgcc gtaggtcggc gccacgccgt cctggcgtgc gcccgacgcg
     3181 gagaacgtgc ggtcgcgcgc gtcgtgccac aggccggcat cgacatacat cgccgggtac
     3241 ttggagagat aacgcccgat ctcccagtcc tccgcgctgg cctgcgcgcg tgcgccggtg
     3301 aagcaggcgg caagcagcgc gacgcgggcc aggatcttca tgcgcgtggt tgttcgtatt
     3361 gccggcggat gtcgggcttg aggatttttc ccgcgccgga cttgggcagc gccggcagga
     3421 agaaatatgc gcgcggcacc ttgtagcccg caagccgcgt cttcagctcg gcgcgcagcg
     3481 ccgcttcgtc ggcggcatgg cccggcttga gcaccaccgc ggccagcccc acctcgcccc
     3541 atttttcgtc ggcgatgccg atcaccgcgc actcggccac gccggcgcat tgcgccagag
     3601 ccgcctcgac ttccgccggg taaacattct cgccgccgga gatgtacatc tccttgctgc
     3661 ggccgacgat gcggtacagg ccgtcggcac ccacgcgcgc gaggtcgccg gtccacagcc
     3721 agccgtcgcg gtacaccttg cgccactgcg cctcgcgctc gaagtagccg ctgcacacct
     3781 gcgggccgct cagcgtgagt tcgccgatct cgtccgcagc gcaggcgctg ccgtcggcgc
     3841 ggcgcacggc cgcctgcagc tgcggcagcg ggcggccgac ggcatcggga tttttctccg
     3901 cctcatccag gccgatggcg aaacagttca cgccgcactc ggtcatgcca tagccctgcc
     3961 tgaagcggat gccgcgcgcg gcgaacggtg cgcgcacggt gggcgggcac ggcgcgccgc
     4021 cggagatggc ccaacgcacc tggctcaggt cggccgctgg gaaatccgcg tgctccgtca
     4081 gcatccggta catggtcggc accatgaaca gcacgctggc gccgtgctgc gcggccaggc
     4141 gcaggtaggc gccgggctcg aagtcgcgca tcagcaccac gcgcccgccg agcttcaaca
     4201 gcggcgtggt gaacacgttg agcgcggcgt ggaagcacgg cgtggcctgg atcgcgcaat
     4261 cctccggcca caggtcccag ccggcggcgg tggcggtgca gttggccagc acctgccggt
     4321 acgagatcag cgcgcccttg ggcaggccgg tgctgccgcc ggtgagcagg atcatgtgcg
     4381 catcgcgctc gtcgagttcc accggcggca gcgcggcgcg cgcggtgtca atccagcttt
     4441 cgtattgatc gagcgcgagc atctccacgc ccagcgtgcg cgcgaacggc agatgcgcgg
     4501 catcggccag catcagcctg ggcttcagat aacgcgtcag cgcctgctgc tcgccggcgg
     4561 acaggcggaa gttgtagggc gtgtagatga agccgagcag cggcgcggcc agcaccagtt
     4621 cgatgtgcgc caggtggttg tgcgcgtaga tggcgacgcg gtcgcccctg gcgatgccga
     4681 tcgaggccag cgccgccgcc agccggcgcg cgcgcaggcc gagttcgccg tagcggtacc
     4741 agcgcccgtc ccaccacagc gccggccgag ccggatcgtg cgccgcgatg aggtcgaggc
     4801 tcatgccgtc attctcacgc agatggcgtt ccacacgtag ccgatgccgg ccgcgcacag
     4861 caccgcgatg tcgcctttcc gcaaacgccc ctgttcgcgc gccagctgga gcgacaacag
     4921 cggatcgatc tggccgagat ggccgtagtc ggaaagatag atcgaacgct ccggcggcac
     4981 gcccacgcgc tcgaggatct gctggtgcgc gctgcgcttc atgtgcagca tggccacgta
     5041 agccgcttcg cgcggccgga tgccgctgcg cgcgcaggcc tgctcgatca cgccgacgaa
     5101 attttccagc gacacgcgtt cgagccgcgc cttcatgccg gcggagtcgc gcacctggaa
     5161 gcggaactgc tcgacgttgt ccgcggtgag cggctcgacg gtgccgccca ccggcacgat
     5221 gacgtcgagc gaaaagctgc cgtcggtgcg gaaggccgag ccgaggattt caaatccgct
     5281 gccgccgcgc gtgaccaccg ccgcgccgcc gccggcagca agattgttga ggaagcgcgt
     5341 ggccggatcg gcgtagtcga tcaggtcgcc gttgcggtag ccgccggcga tcagcacggt
     5401 ggacacgccg tcggccttga tcatgtcgcg cgccagcttg agcgccagca cagcggtgcc
     5461 gcatttctgg ccgagatcga aggcgtaggc gttgaccgcg ccgagctcgt gcgccgcctg
     5521 gatgccggcg gtccacaccg ggtattcctt gtgctcctcg gtgatgcaga tcagcgcgtc
     5581 gacttcgcgc gcgtcgatgc gtgcgtcggc cagcgcggcg cgcgcggcgc gcacgcccat
     5641 ggcattggta tggtcttcag gtccgggaac gggcttggcg gtgatgccga gcttgtcgcg
     5701 caccacggcc tccggcaggc cgctggcggc ggcaatgtcg gcggcgctgt gccgcggctc
     5761 gggaatgtag atgcctaagc cgcggacgat catcagctct cgtgcttgcg cggcgccatg
     5821 ccgcgcgcga cgaagtccat caggtcatcc atctgcgccg cgtccggcgc cttgccttcc
     5881 cacaggcagt ggcgcatgcc gatgaaatgg ccgatgccca tgatcgacca ggcgcgcagc
     5941 tcggcgttgc ccggcgacag ctcgctgcgc gcggtggccg cttcgatcgc ctgcgtgtag
     6001 cccgaggcca gccgctggta gtactcgcgg aaggcgcctt cgtcgacgaa ctggcattcc
     6061 tgcacgatgc ggtacaggcc cgggtgctgc tgggcgaatt cgaggaaggc ttcgaggccg
     6121 gcgcgctccg ccgccatgcg cggcgtggcc ttgcccaccg cttcggccat gtgcttgcgc
     6181 agcgcgtggc cgatgtcgcg caccagcgtg acgaagattt cttccttgct gtggaagtaa
     6241 aggtagaagg tgccctggcc gacgtcggcg cgggtggtga tcgagctgac cgaggccgcg
     6301 tggaacccct tgccgccgaa ttccttctcc gccgcgtcga gcagcttgcg ccgcgtggct
     6361 tcgccgcgcg cggtggccgg gacgatggga gcgtgagtgg acgacatcgg gacggaagca
     6421 gcttgatatg acatgtgatt caactctcag gattgttgga tcggcgcagg ccgtcgtcaa
     6481 ctccgcacgg cgtgcgaagc ggtgattcga gcgtaggaaa tgttcgatga catgcgcagg
     6541 cgattctgcg cgtaatggtg aagcttgacg cggggttcag ggatgaccgg cgctgcgcgg
     6601 cggaattccg gtatggagaa aatccaccag gccctgtgga aatctcagct gaccacgcgc
     6661 agccagcgcg gtttcttgcg cgagtccacc gcgcgctgct tgagcagcgc ggtgcagtct
     6721 tcaaatgcgc gcgagatgcg cgtggcagtg cggatgcgat cgagcttggg ccaggtggcg
     6781 cgctcgcctt cgcggatgta cgcctggatt tcttccgcgc tcgggttggc gaacatgttg
     6841 agcagctgcc ggggcgtctg ccgcggaaag atgttgatgt cgcccgagta gcgctgcttg
     6901 acgatgttgt cgaccttgtt gatcacgcgc ccgccgccgt acgggtccag gtgcttgcgc
     6961 gccagcttga gcgcgccgcg tccgccgtgc gccaccagtt ccctggccag cggcgccagg
     7021 ccggtcgacg gcagcttgtc gctcatgaac ggcacgatgt gcgggttggt ctggctgacg
     7081 atgtagtggt tgacgttgtg caggcgcgcg gtgcgcagca tcggcaggtc ggctgacagc
     7141 gtcccatcga cccagcgctt gctcggcatg taaggcgtga cgcggcccag gtaatcgcgc
     7201 ccttccagca tcaccggcgg gaacacgccg ggcaccgcgc acgaggcgag gatggctttc
     7261 gacagcagca cgtgcggcga ggtcaggtaa ttcatcagcc ggccctgctg gtgcggctcg
     7321 gccggcgaga tggtcacgcc gacgatgcgc cgggtgcgct cgaacgcttc gacgaaagtc
     7381 tcgccgccga tgttgtcggc caggcatttc tccagcgcgc cggcgtccat caccgcgcgg
     7441 cccttgatgg cccggcgcag cgtcacgctc tggaacgcct gcaggttgag cccgcccggc
     7501 tcgaacacgc gcttgagcgc ggcatcgtcg cgcgtgccgg ccacgccggc gatgatcgaa
     7561 cccgccgacg agcccgacag cacgcgcggc agcaggccct gctcctgcag cgccttgatc
     7621 acgcccaggt ggaacatgcc cagcgtggcg ccgccggaaa gcatcagcgc gctgcggccg
     7681 tacaccgtgc cggtgcgctt gaagaacagg atcttctcgt cgtgcgagaa gtcgggaaag
     7741 tcgccggcgc agatgtaatc gaggcagcgc gccacctcat cgacgtactg ctcgatcagc
     7801 tgcttggtgc ccacgcgcgc ctgcgcatac agcgcggcat tggaaatatt gccgaggttg
     7861 ccgtgcaggc cttcatacag gttgaacacc agctggcgca cgtcgccgcg cgtgcgcagc
     7921 gcgcgcatct gcgccaggcg ctccttgatc agcaggtggt cgaagtcgtc gctggtgtcg
     7981 tcctgcttcc aggcgtcggc gccctcgagc cgatcgagtt ccagcgcgat ctctttccac
     8041 gtcgcgtagt cggcggcctg gtcgagttcg cggcggcgtg cggcaaccag tttccgcgcc
     8101 acgtgggtac gcatgagcat gcggcaatgg tagtgcattt tgtgcagcgc accgtcggcc
     8161 gtttgcaccg cgctgccgct gccacggtct gttcatcgtg cagacggagt tttgggcgag
     8221 aataggccgc accgcactgc ccgtggtcct gttataattt gttgtatttt tataaaaacg
     8281 attaataatt tataaccgcc cggacggccg tgcggtgacg tgaaaaaacc gccccgatca
     8341 gctgatggaa tctgccgcgc ctattgttcc cgccgagacg acctggtcgc gcctgcgcag
     8401 ctggctgccg acgctgatcg cgctcgcgct gatcgccatg gtgctgctgg ccatccgctc
     8461 gctgctgcgc gaagtgtcgt acgacgaagt ggccgacgcg gtgctgacca ccaggcgcag
     8521 cgccatcgcc tggtcggtgc tggccactgt cgcctcgtac atctcgctgc tcggctatga
     8581 ctggtcgggc gcgcgctacg caggcatccg cctgccgcgc ggcacgctgg cgctggccag
     8641 cttcgtcggc tacgcactgg gcaacagcgt cggcctgggc gcgctcaccg gcggcgcggt
     8701 gcgcttccgc atctactcgg cggcgggcat cgaggcgggc cgcatcggca agctggtgat
     8761 gttcatcgtc agtgccttcg gcgccggtgt gaccgtggtg gcggccgcgt cgctgatgtg
     8821 ggcggccacg catgtcgggc acgtggcgca cctgtcgccg ctgctgctgc gcgtcatcgc
     8881 cggcagcgtc ctggcgctgg cggccggctt catcgtgctg tgcttcgccc gccgcgaagt
     8941 gttgctgctc ggccgctggc agctgcagct gccgaccggc ggggtggcgc tggcgcagct
     9001 ggcggtgtcg gtggccgaca tcatcgccgc cgccgcagcg ctgtggttcc tgctgcctgg
     9061 cgcacgcgaa tcgtttccgc agttcgtcgc cttctattcc attgcgctgg ccgctggcgt
     9121 catctcgcac gtgccgggcg ggctgggcgt gttcgagggc gtgctgctgc tggcctacgg
     9181 ccaccgcgcg ccgctcgacg cgctggccgg cgcgctgctg ctgtaccgcg ccatctactt
     9241 catcggcccg ctgttcctgg ccatgctggt gctggcgttc tacgaactgc gcgccagcgt
     9301 ggccggcgtg ggccgcgccg cggcgcagct gtcgccgatg ctgctgtcgg tgtggacgct
     9361 gatcgtcggc atcatgctgc tggtgtcggg cgtgacgccg gccaccggca acgccaccga
     9421 gctgctctcg ctgcacgtgc cgctgcagat cgtcgaggcc tcgcacttca tcggcagcat
     9481 tgccgggctg ggcctgctgt tcgtggcgcg cgggttgctc taccggctcg atgccgcgtg
     9541 gtgggccgcg ctggtgctga ccatcgtcgc cttctggctg gcgctgccca agggcatcgc
     9601 gctgaccgaa atggcggtgc tggccttcct cgccttcaac ctgatcgtcg gccggcgcga
     9661 attcgaccgc cgcgcctcgc tgttctcgca ggtgttctcg cgcggctgga tgctgatggt
     9721 gggcgcggtg ctggtcggcg tgggctgggt catgttcttc gtctaccagg acatcgacta
     9781 cacgcacgaa ctgtggtggc agttcgcctt cgacgcgcac gcgccgcgct cgctgcgcct
     9841 gctgctggcc gcggtggtgt cgacgctcgg cgtggccatg tggcaatggt tccggccgta
     9901 ctcgggcaat gccggcggct gcagcccggc cgacctcgaa cacgcccgcg gcgtgctgcg
     9961 cgagcagccg cgcgcggacg cctgcctggc gctcaccggc gacaagagcc tgctgttttc
    10021 caggtccggc aaggcgtttc tcatgtacgg caagcgcggc cgcacctggg tatcgctgta
    10081 cgacccggtc ggcgcgcagc aggatctgcc cgaactggtg tggagcttca tggaactggc
    10141 ggcggcgcac ggcggccgcg cggtgttcta ccaggtgcgt ccgcagaatc tctcgctcta
    10201 cgtcgacacc ggcctgcgcc cgttcaagct gggcgaggat gcgcgcgtgg cactggcgtc
    10261 gttcaccacc aagggctcgg ccaacgcgca cctgcgccag gcgatcaaca agggcgagcg
    10321 cgaagggctg tcgctggaga tcgtgccggc cgaaggggtg gcggcacagc tgccggaact
    10381 ggcgcgcatc tccgacgcct ggctggagac gcacaacacg cgcgagaagc atttctcgct
    10441 cggcgccttc gatccgaact atctggcggc gctgccggtc gcagtggtgc gccgcgccgg
    10501 cgcgccgatc gccttcgcca acatcctgtg cacggcgttg aaggacgaag cgcgcgtcga
    10561 cctgatgcgc tacgcgcccg gtgcacccaa cggcaccatg gattacctgt tcgtgaagct
    10621 gatgctgcat ttccaggccg agggctacgc gcatttcggc ctgggcatgg cgccgttctc
    10681 cggcctggtg cggcatccgc tggcgccgcg ctggcaccgg ctgggcagcc tgctgttcgg
    10741 cctgggcgag catttctaca attttcaggg cctgcgctcg ttcaaggaaa aattccatcc
    10801 gcagtgggag ccgcgctatc tctgttcgcc cggcggcagc gcaccgctgt tcgcgctggc
    10861 cgacattgcc tcgctcacca gcggcggact caagggagtg atcagcaagt gaagcgctgg
    10921 ctctggatgc tgctgctgtg cgcgggcgcc gcgcaggcgc aggagcaggt ggcgcacggc
    10981 cgcttcgacg acgtgatgct gtatcgcccg gccggcgagc cgcagtcctt cgtgctgttc
    11041 ctgtccgatg ccgacggcgg cgatgcgccg accacgcgca tgatgcgcac gctggtcgcg
    11101 cgcggcgcgc tggtggccgg catcaacacg cgcgatttct tcgccgccat ggaagccgac
    11161 ggcgcgccgt gccagtacgc cgacggcgac ctggagaacc tgagccattt cctgcaggcc
    11221 tatgcgcagg tgccgggcta cctgccgccg ctgctcgccg gcaagggcgt gggcgcctcg
    11281 ttcgcctacg cggtgctggc gcaggcgccc aagggcatgt tcgccggcgc actcgcgctg
    11341 ggcttcacgc gcgagctgcc gctacaccag aaaacattgt gcgaactgcc caccgcgcag
    11401 gcgcgcgccg acggcagcgg gctcgacctg ctacccgccg cgcgcctgtc cggcccgctg
    11461 cgcgtgctgc acggcgaggc cgacgacgtg gcgccgctgg ccggcatcgc gtcgttcatg
    11521 gcgctggtgc cgcaggcaca gaccattccg ctgcccaacg tcggccacgg cttcggcaac
    11581 tggcgcgact ggcaggcgct gctcggcgtg gcctacgagg aactggtcgc gccgatccgg
    11641 cccgccatgc gcgcgccggc gggcgacgtg gccgatctgc cggtgatcga gctgccggtg
    11701 cagcagtccg gcgacagctt cgcgctgatc atctccggcg acggcggctg ggccggcctg
    11761 gacaagaacc tggccagcgc gctgctggcg cgcggcatcc cggtggtcgg cctcgactcg
    11821 ctgcgctact tctggtcgcc gcgcacgccg gagggcgtgg cgcacgatgt cgaacgcctg
    11881 atccgccact acagcgcgcg ctggcagaag aagcgcgtgc tgctgatcgg ctactcgcag
    11941 ggcggcgacg tgctgccgtt cgcctacaac cggctgtcgc cggcggtgcg cgcgcaggtg
    12001 gcgctggcgg tgccgatctc gatcagcgac cgggccgcct tcgagttcca catgaccaac
    12061 tgggtcggcg gcaagggcga cgtgccgacc atgccggaaa tggacaagct ggcgggcaac
    12121 agcacgctgt tctgcgtcta tggcgccgac gacaaggaat cgctgtgctc gcaactcgat
    12181 ccaaagcgct tccgcgtgaa gtcgctgccc ggcgcgcatc acttcaacgg cgactacgcg
    12241 aagctcgctg ctacggtttt agaagcactg ccgaaggact gaagtggaag gcctggtcat
    12301 tcccgcgctg tgggcactgg ccgtgctgct ggtgctcggc ggcatcgtcg gcaccgtgct
    12361 gccggtgctg ccgggcgcgc cgatgatgtt cggcggactg tggctcgccg cctggctgga
    12421 ccactatgcg catgccgggc tgatcacgct gctgctgctg gcgctgctca cgttcatctc
    12481 catggcagtg gactggatcg ccggcgcgct gggggcgaaa aaggtcggcg cgagcccgcg
    12541 cgccatcagc ggcgccgccg cgggcagcct gctcggcggc atgtttctgt ttccgtgggg
    12601 tctgc
//
LOCUS       DASZZC010000378         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_224768,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000378 DASZZC010000000
VERSION     DASZZC010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224768"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VHE37_08140"
     CDS             <1..1296
                     /locus_tag="VHE37_08140"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HVT35538.1"
                     /translation="KVGLIGLGTVGCGVVRVLRQNAEEIARRVGRPLVITHASARDLT
                     RKRECELNGITIVSDPMSVARDAEVDVVVEVIGGKSPAFELMQAAITRGTHVVTANKA
                     LLAERGNEIFAAARAKGVMVAFEAAVAGGIPVIKAIREGLAGNRIDALAGIINGTSNY
                     ILTQMTSNGETFASALKTAQKLGYAEADPTFDVEGIDAAHKLTILAAIAFGMPLSLPA
                     VNIEGITKVTPADIKIAQALGYRIKHLGIAKRGERGAELRVQPTLIPEDKLLAKVDGV
                     LNSVLLTGNAVGQVGFYGRGAGGEATASAVVADLVDVARSLGAPARHHVPALAFQPEG
                     VRELPLLPIGEIRSAHYLRLTVADEPGVLQAITGIFAEKQISIEAIIQREPREDEDAT
                     VAVITSEVVEEHFNQALARIQALPFVRKSLSRFRVEHFA"
     gene            1322..1930
                     /locus_tag="VHE37_08145"
     CDS             1322..1930
                     /locus_tag="VHE37_08145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVT35539.1"
                     /translation="MNSGKPTAFVPVTLRGVHVTLEPLSMSHHAALTEAGADPAISRW
                     FPKPVTGAQNMREFIDEAMRMLAEGKSVPFAIRAGADYVGSTRFANIEATHRRAEIGW
                     TWLTPSVQRSAVNSECKYLLLRHAFEEQGCNRVELKTDALNEKSRNAILRIGAKQEGI
                     FRQHMVVQGGRLRDTVYFSIVRDEWPAVKAALEATLNRGSKS"
     gene            1927..3030
                     /gene="thrC"
                     /locus_tag="VHE37_08150"
     CDS             1927..3030
                     /gene="thrC"
                     /locus_tag="VHE37_08150"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVT35540.1"
                     /translation="MTRYTGLVDHYRSRLPLPAEAKAVALCEGNTPLIQLQNIPKSVG
                     FTGELYVKFEGLNPTGSFKDRGMTVAVTQAAHAGAKAIICASTGNTSAAAAAYAARAG
                     ITAFVLIPEGKIALGKLAQAVVHGAVVVQIQGNFDDGMRLVKELGQTTPVAIVNSINP
                     YRLQGQKTASFEIVEALGRAPDYHCLPVGNAGNISAYWMGYSEAVGLRTSASMDGYAL
                     GSAKHGRPKMCGYQAAGAAPFLRGGPVKNPETVATAIRIGDPQSWDHAWKAQKESGGW
                     FAEFTDAEILATQKLLSEKEGVFCEPASATSLCGALHDIKLGKIPKNATVVCTLTGNG
                     LKDPDIVMKDGLKGLIQVPAERSAVEQALLARM"
BASE COUNT          553 a          982 c         1058 g          446 t
ORIGIN      
        1 aaggtgggcc tgatcggcct gggcaccgtc ggctgcggcg tggtgcgcgt gctcaggcag
       61 aatgcggaag agatcgcgcg ccgtgtcggc cgcccgctgg tgatcacgca cgcgtccgcg
      121 cgcgacctca cgcgcaagcg cgaatgcgag ctcaacggca tcaccatcgt ctccgacccg
      181 atgtcggtgg cgcgcgacgc cgaggtcgac gtggtggtgg aagtgatcgg cggcaagtcg
      241 ccggcgtttg aactgatgca ggccgccatc acgcgcggaa cccacgtggt caccgccaac
      301 aaggcactgc tggccgagcg cggcaacgag atcttcgccg ccgcgcgggc caagggcgtc
      361 atggtcgcgt tcgaggcggc cgtggccggc ggcattccgg tgatcaaggc catccgcgag
      421 ggcctcgccg gcaaccgcat cgacgcgctg gccggcatca tcaacggcac cagcaactac
      481 atcctcacgc agatgaccag caacggcgag actttcgcca gcgcgttgaa gaccgcgcag
      541 aagctcggtt atgccgaggc cgatccgacc ttcgacgtcg aaggcatcga cgccgcgcac
      601 aagctcacca ttctcgccgc catcgccttc ggcatgccgc tgtcgctgcc ggcggtgaac
      661 atcgagggca tcaccaaggt gacgccggcc gacatcaaga tcgcgcaggc gctgggctac
      721 cgcatcaagc acctgggcat cgccaagcgc ggcgagcgcg gcgcggaact gcgcgtgcag
      781 cccacgctga tcccggaaga caagctgctg gcaaaagtcg acggcgtgct caactcggtg
      841 ctgctcaccg gcaacgcggt cgggcaggtg ggcttctacg gccgcggcgc cggcggcgag
      901 gcgacggcgt ctgccgtggt ggccgacctg gtcgacgtgg cgcgctcgct gggcgcgccc
      961 gcgcgccatc acgtgccggc gctggcgttc cagccggaag gcgtgcgcga actgccgctg
     1021 ctgcccatcg gcgaaatccg cagcgcccac tacctgcgcc tgaccgtggc tgacgagccg
     1081 ggcgtgctgc aggccatcac cggcatcttc gcagagaagc agatcagcat cgaggcgatc
     1141 atccagcgcg agccgcgcga ggacgaagac gccaccgtgg cggtcatcac gtccgaagtg
     1201 gtcgaggagc atttcaacca ggcgctggcg cgcatccagg ccttgccgtt cgtgcgcaag
     1261 agcctgtcgc gcttccgcgt cgagcatttc gcgtaaagac gggattcgag attcgggatt
     1321 cgtgaacagc ggaaagccaa cggcatttgt gccggtgacc ctgcgcggcg tgcatgtcac
     1381 gctggagccg ttgtcgatgt cgcaccacgc agcgctgacc gaagcgggcg ccgatccggc
     1441 catttcgcgc tggtttccga agccggtgac cggcgcgcag aacatgcgcg agttcatcga
     1501 cgaggccatg cgcatgctgg ccgagggcaa gtcggtgccg ttcgccatca gggcgggagc
     1561 ggattacgtg ggcagcacgc gctttgccaa catcgaggcg acgcaccggc gcgcggagat
     1621 cggctggacc tggctgactc cctcggtgca gcgcagcgcg gtcaactcgg agtgcaagta
     1681 cctgctgctg cggcatgcgt tcgaggagca gggctgcaac cgcgtggaac tgaagaccga
     1741 cgcgctaaac gaaaaatcac gcaacgccat cctgcgcatc ggtgcgaaac aggaaggtat
     1801 cttccgccag cacatggtgg tgcagggcgg gcgcctgcgc gacacggtgt acttcagcat
     1861 cgtgcgcgac gagtggccgg cagtgaaggc ggcgctggaa gccacgttga accggggaag
     1921 caagtcatga cgcgttatac cgggctggtc gatcactacc gttcccgcct gccgctgccg
     1981 gcagaggcaa aagccgtcgc gctgtgcgaa ggcaacacgc cgctgatcca gctgcaaaac
     2041 atcccgaagt cggtgggctt caccggcgag ctgtacgtga agttcgaggg cctcaatccc
     2101 accggttcgt tcaaggatcg cggcatgacc gtggcggtga cgcaggctgc gcatgcgggc
     2161 gccaaggcga tcatctgcgc gtccaccggc aacacctccg cagccgcggc cgcctacgcc
     2221 gcgcgcgcgg gcatcacggc gttcgtgctc attcccgaag gcaagatcgc gctgggcaag
     2281 ctggcgcagg ccgtggtgca cggcgcggtg gtggtgcaga tccagggcaa cttcgacgac
     2341 ggcatgcggc tggtgaagga gctgggccag accacgccgg tggccatcgt caactcgatc
     2401 aacccgtatc gcctgcaggg gcagaagacg gcctcattcg agatcgtcga ggcgctcggc
     2461 cgtgcgccgg attaccactg cctgccggtg ggcaacgccg gcaacatcag cgcgtactgg
     2521 atgggctaca gcgaagcggt cggcctgcgc acctccgcca gcatggatgg ctacgcgctg
     2581 ggcagtgcga aacatggccg gccgaagatg tgcggctacc aggctgccgg cgcggcgccg
     2641 ttcctgcgcg gcggcccggt gaagaatccg gagacggtgg ccaccgccat ccgtatcggc
     2701 gatccgcagt cctgggatca cgcgtggaag gcgcagaagg aatcgggcgg ctggttcgcc
     2761 gaattcactg acgccgaaat cctcgcgacg cagaagctgc tgtcggaaaa ggaaggtgtg
     2821 ttctgcgagc ccgcgtcggc cacttcgctg tgcggagcac tgcacgacat caagctcgga
     2881 aaaattccta agaacgcgac ggtggtgtgc acgctcaccg gcaatggact caaggacccg
     2941 gacatcgtga tgaaggatgg cctcaagggc ctgatccagg tgccggccga gcgcagcgcg
     3001 gtggagcagg cgctgctggc gcggatgtag gagcgcgcc
//
LOCUS       DASZZC010000379         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_733251,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000379 DASZZC010000000
VERSION     DASZZC010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733251"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2449
                     /locus_tag="VHE37_08155"
     CDS             <1..>2449
                     /locus_tag="VHE37_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35541.1"
                     /translation="AATPAAAPESAVPAAEAPAAAEAPVEAPAPAPTGDVATIPVKTP
                     EAEEKPASDGHTQRLDAIQVTGSRIRRTDYETAQPVTLINRDQIDRSGLTNIGDLLAR
                     ISAAGASVNTTFATLALSGGETNLDLRNLGSSRVLVLVNGHRWVNGLNSTGTSAVDLN
                     NIPTSIIDHIEVLKDGASAIYGSDAIAGVVNIITRKDYDGQEVSAQYGAYEQGDGAKS
                     QYTASFGRVLSSTSVFANFTYSQEDVVKTTTRAISAYPGVGAGTTRLSSIGPNGLFEF
                     IPDPKNGAAFQCPNLVGDLAAGVLADPAGNSPVALPFGLPVGGIKLPQPVVNGIDMIP
                     AGLQLCELIHNPKAAVDTYPLNYRTFDPTTDGFNRYQDSNLQNPSKRGALFMQVKQEI
                     LDNLNFTFEGLYDERNSESQLRPQALEVGDLAGLSSYVDQTQMYNPFGQNIGKGDPVL
                     TPQLGLGIGSGVAALRLTSFPPVVSKDRYKTLRLGGDFDGTFDVLSQTVTWDVGYSHS
                     YNHRREDGTGDLRADHLALALGPASACTGDCVAADIFHGNAGLTPAMINYLALYRTNH
                     TKQSQEDAYGNVSTEFGALPLPGGPVALAAGVEYRHDEYSDDPDWQLQQGLSTGNNSV
                     RTQGSAIAKEMYAELGLPLLKNLPAIETLELSLAARHSIYRGFGGTNTGKAGIRWKPV
                     NDLLLRGTYSTAFRAPSVGELFLGDSDSFETVTDPCATDARNQPNVAANCTADGVSTN
                     VSGPPANQLRSTVGGNQQLRPETARTLTYGLVFEPSFVPNLNITVDYYRIKLKNIIAE
                     PGDQFIIDSCYDNSQRAL"
BASE COUNT          473 a          851 c          728 g          397 t
ORIGIN      
        1 ggcagcgaca cccgctgccg cccccgagag cgccgttccc gcagcggagg cacccgcagc
       61 cgccgaggcg ccggtcgaag cgcctgcgcc cgcgcccacc ggcgatgtag ccaccattcc
      121 ggtgaagacg cccgaagcag aggaaaagcc ggccagcgat ggccacacgc agcggctcga
      181 tgcgatccag gtgactggtt cgcgcatccg tcgcaccgac tatgagacgg cacagccggt
      241 gacgctgatc aatcgcgatc agatcgaccg ctccggcctg accaatatcg gcgatctgct
      301 ggcgcgcatc tcggcagctg gcgcttcggt caacaccacc ttcgccacgc tggcgctgtc
      361 gggcggcgaa accaacctgg acctgcgcaa tctcggctcc agccgtgtgc tggtgctggt
      421 caatggccat cgctgggtga acggtttgaa ctctaccggc acctctgcgg tggacctgaa
      481 caacattccg acgtcaatca tcgaccacat tgaagtgctg aaggatggcg cttccgcgat
      541 ctacggctcc gacgccattg ccggcgtggt gaacatcatc acgcgcaagg attacgacgg
      601 acaggaagtg agcgcacagt acggcgccta cgagcaggga gacggcgcca agtcgcagta
      661 caccgcttcg ttcggccgcg tgctgtccag cacatcggtg tttgccaact tcacctactc
      721 gcaggaagac gtggtcaaga ccaccacccg cgccatctcg gcttaccctg gcgtcggcgc
      781 cggcaccacc cgcctgagct ccatcggccc caacggcctg tttgagttca tccccgaccc
      841 gaaaaacggc gcggcgttcc agtgccccaa cctggtcggc gatctcgccg ccggcgttct
      901 cgccgaccca gccggcaact caccggttgc cctgcccttc ggcctgccgg tgggcggcat
      961 caagctgccg cagccggtgg tgaacggcat cgacatgatt cccgccggcc tgcagctgtg
     1021 cgagttgatc cacaacccca aggcggcggt cgacacctac ccgctgaact accgcacctt
     1081 cgaccccacc accgacggct tcaaccgtta ccaggacagc aacctgcaga acccgagcaa
     1141 gcgcggtgcg ctgttcatgc aggtaaagca ggagatcctc gacaatctca atttcacctt
     1201 tgaaggcctg tacgacgagc gcaactccga gtcgcagctg cgtccgcagg cgctggaagt
     1261 aggcgatctg gctggcctca gcagctatgt cgaccagacc cagatgtaca acccctttgg
     1321 ccagaacatc ggcaagggcg acccggtgct gacgccgcag ctgggcctgg gcatcggctc
     1381 cggcgtggct gcgctgcgct tgaccagctt cccgccggtg gtttccaagg atcgctacaa
     1441 gaccttgcgg ctgggcggcg acttcgacgg cacctttgac gtgctgtcgc agaccgtcac
     1501 ctgggacgtc ggctacagcc actcgtacaa ccaccgcagg gaagacggca cgggcgacct
     1561 ccgtgcagat caccttgccc tggccctcgg tccggcttcg gcctgcacgg gagactgcgt
     1621 ggcggccgac atctttcacg gcaatgcagg cctgacgccg gccatgatca attacctggc
     1681 gctgtaccgc accaaccaca ccaagcagtc gcaggaagat gcatatggaa acgtgtcgac
     1741 agaattcggc gcgctgcccc ttccaggtgg ccccgtggcc ctggcggcag gtgtcgaata
     1801 tcgccacgac gaatacagcg acgatccaga ctggcagttg cagcagggcc tgtccaccgg
     1861 caacaactcc gtgcgcacgc agggctcggc gatcgccaag gaaatgtatg cggaactggg
     1921 actgccgctg ttgaagaatc ttcccgcgat cgagacgctg gagttgagcc tcgcggcacg
     1981 tcactccatc taccgcggct tcggcggcac caataccggc aaggcgggca tccgctggaa
     2041 acccgtgaac gacctgctgc tgcgcggcac ctactcgacg gctttccgcg cgccgtcggt
     2101 gggtgaattg ttcctgggcg actccgacag cttcgagacg gttaccgacc cctgcgcaac
     2161 cgatgcgcgc aaccagccca acgtggccgc caattgcacc gccgacggcg tgtcgaccaa
     2221 cgtttcgggc ccgccggcca accagttgcg ctcgacggtg ggcggcaacc agcagctccg
     2281 gccggaaacc gcgcgcacgc tcacttacgg actggtgttc gagccttcgt tcgtgccaaa
     2341 cctgaacata acggtcgact attacaggat caagctgaag aacatcatcg ccgagccggg
     2401 cgaccagttc atcatcgact cgtgctacga caactcgcaa cgtgcgctg
//
LOCUS       DASZZC010000380         4734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_35412,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000380 DASZZC010000000
VERSION     DASZZC010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35412"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VHE37_08160"
     CDS             <1..1592
                     /locus_tag="VHE37_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HVT35542.1"
                     /translation="RFDAQAQAFVPQGGVQQASSVGSAAQVDARHAPAPLWSVDVSAL
                     GRTPAYSWVDLASDVTEGDIRLAVREGFAAVEHLKRYTTTGMMADQGKTSNVNAIGIL
                     GTLAGKAPGEVGTTRFRPPFNPVTFGAIAGQSVNDFYQPLRRLPTDALERDAGGVMED
                     YGGWWRPSHFPHKGESEQDAVMREVRAVRSGVGIVDYSPLGKILVHGPDAGTLLQRIY
                     VNNMRTLKPGFCRYGLMLGEDGIVMDDGVLTRWDEHTWQVGTTSGQAERVAEWLEEWL
                     QCEWVDLKVAVEPVTTQWSVMMVAGPQARTLLQRLGSDMDLSAEAFPHMTARHGTLAG
                     MPLRIMRVSFTGELSYEVSVPWRHGAALWQRLLEAGHDLGVVPFGIEALMVMRIEKGF
                     LHVGTETDGATMPQDIGFGEIIARKADDFAGRRSTMTPEGRRADRRQLVGLAPADGKT
                     LLPVGGHLRGNGAARSQGWVTSSVMSPTLGRPVAMALLENGRARLKETIEVYDALSAS
                     AIAAVVVETAAYDPTGARLHG"
     gene            1585..2175
                     /locus_tag="VHE37_08165"
     CDS             1585..2175
                     /locus_tag="VHE37_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35543.1"
                     /translation="MANDIPAHGAPLAACSALGVRAGTPVAPLVEDESLCIVEEPLLA
                     AVSLRQYRAQPDSSALVAALAEFKVAAPAQPNAMTGDSRHGAAWIEPHAWLVTALGDL
                     PVPVPRAGLLVTDVSDRYAALRISGARARSVIAAGCDPAILAPMRAARTRFGGVATAL
                     IQQWGEDDYRVLVDVSLAQTVAAWLRQAAAFSRELS"
     gene            2172..2570
                     /locus_tag="VHE37_08170"
     CDS             2172..2570
                     /locus_tag="VHE37_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35544.1"
                     /translation="MSKPLFMSPEHVRLMNERLAGDVASKSACAALPRRYQLAYRLED
                     GERVVWWTTVFDPAGGVRFHLAPPEGDADLQFSGSYWAMIEFTQRSKQGDAGSFPLTT
                     GGDLNTYAIIMPAFAAAQAAATLDTEFPAR"
     gene            complement(2716..3948)
                     /locus_tag="VHE37_08175"
     CDS             complement(2716..3948)
                     /locus_tag="VHE37_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT35545.1"
                     /translation="MTSRKTAFINAQVFDGENPVRSAPSTVVIADGVIERVTPGHAPG
                     EGADIIDCTGRTLMPGLIDAHVHVYANSLKLSPPEAPMSYRAHHAARFLSHILGCGFT
                     AVRDVAGGDQGLAMALRDGYLQGPRYFYGGPALSQTGGHGDMRAPGTEDLCPCCSGSS
                     MVGLIADGVDACRTAVRDQFRKGAHHIKIMASGGVVSPTDPLEYPQYSTDELRAIVDE
                     CDRRGSYACAHCHPAAAVRRCVECGVRCIEHGTLIDQPTADFVAEKGAFVVPTMATIF
                     ALLADGPKLGLLGSSYEKLQRVADKAVAGLSIMKRAGVRMGFGTDLLGSQYVLQGTEF
                     TLRRQALSAADILISATSVNADIVQMKDKLGVVKAGALADLLVVDGNPLDDIGVLASN
                     GKHLTHIMRGGEFIKRAN"
     gene            4221..>4734
                     /locus_tag="VHE37_08180"
     CDS             4221..>4734
                     /locus_tag="VHE37_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HVT35546.1"
                     /translation="MSTSMRRRALPVLLLLFGGRALAQDAGGQVDASAPAAQAEAPAA
                     PDANAAADTAAPVATIPVNSKSTAAPAAAPASDDGSTRLEDVVVTATKRREASREVPI
                     SINAFRGEDLEKRGAKGLEEILQFAPGVSINKNTSADNNYVSIRGVSTNSNNNVYTRP
                     TGLFLEDASLS"
BASE COUNT          729 a         1591 c         1683 g          731 t
ORIGIN      
        1 tgcgcttcga cgcgcaggcg caggccttcg tgccgcaggg cggggtgcag caggcgagct
       61 cggtgggatc ggccgcgcag gtggacgcgc gccatgcacc cgcgccgctg tggtcggtgg
      121 acgtgagcgc gctaggacgc acgccggcct attcatgggt cgatctcgcc agcgacgtga
      181 ccgagggcga catccgcctg gccgtgcgcg aaggcttcgc cgcggtggag cacctcaagc
      241 gctataccac caccggcatg atggccgacc agggcaagac ctcgaacgtc aacgccatcg
      301 gcatcctcgg cacgctggca ggcaaggcgc cgggcgaagt gggcaccacg cgtttccgcc
      361 cgccgttcaa cccggtgacc ttcggcgcca tcgccggaca gagcgtcaac gatttctacc
      421 agccgctgcg caggctgccg accgacgcgc tggagcgtga cgccggcggt gtgatggagg
      481 actacggcgg ctggtggcgg ccctcgcatt ttccgcacaa gggcgagagc gagcaggacg
      541 cggtgatgcg cgaagtgcgc gcggtgcgca gcggcgtggg catcgtcgat tattcgccgc
      601 tgggcaagat cctggtgcac ggccccgacg ccggcacgct gctgcagcgc atctacgtga
      661 acaacatgcg cacgctcaag cccggcttct gccgctacgg cctgatgctg ggcgaggacg
      721 gcatcgtcat ggacgacggc gtgctcacgc gctgggacga acacacctgg caggtcggca
      781 ccaccagcgg ccaggccgag cgcgtggccg agtggctgga ggaatggctg cagtgcgagt
      841 gggttgacct gaaggtggcg gtggagccgg tcaccacgca gtggtcggtg atgatggttg
      901 cgggaccgca ggcgcgcacg ctgctgcagc gcctgggcag cgacatggat ctctccgcag
      961 aagcgttccc gcacatgacc gcgcgtcacg gcacgctcgc cggcatgccg ctgcgcatca
     1021 tgcgcgtgag cttcaccggc gaactgagct acgaggtgag cgtgccctgg cgccacggcg
     1081 ccgcgctgtg gcagcgcctg cttgaagccg ggcacgacct gggcgttgtc ccgttcggca
     1141 tcgaggcgct gatggtgatg cgcatcgaga aaggtttcct gcacgtgggc accgagaccg
     1201 acggcgccac catgccgcag gacatcggct tcggcgagat catcgccagg aaggccgacg
     1261 atttcgccgg ccgccgttcc accatgacgc ccgaaggccg ccgcgccgac cgccgccagc
     1321 tggtcggcct ggcgcccgcc gacggcaaga cgctgttgcc ggtcggcggc cacctgcgcg
     1381 gcaacggggc cgcgcgctcg cagggctggg tgacctcgtc ggtgatgagc cccacgctcg
     1441 gccggccggt ggcgatggcg ctgctcgaga acggccgcgc gcggctgaag gagaccattg
     1501 aggtctacga cgcgctgagc gcatcggcca tcgccgcggt ggtggtcgag accgcggcct
     1561 acgatcccac cggagcgcgc ctgcatggct aacgacatcc ccgcacatgg cgcgccgctg
     1621 gccgcgtgct cggcactcgg cgtgcgcgcc ggtacgccgg tggcgccgct ggttgaagac
     1681 gaaagcctgt gcatcgtgga ggagccgctc ctggccgcgg tgagcctgcg gcaataccgc
     1741 gcgcagccgg attcgtccgc gctcgtcgcc gcgcttgcag aattcaaggt ggccgcgccg
     1801 gcgcagccca atgccatgac cggcgacagc aggcacggcg ccgcatggat cgagccgcac
     1861 gcctggctgg tcaccgcgct cggcgatctg ccggtgcccg tgccgcgcgc cggcctgctg
     1921 gtcaccgatg tttccgatcg ctacgccgcg ctgcgcatca gcggtgcgcg cgcacgcagc
     1981 gtcatcgccg ccggctgcga tccagcgatc ctcgcgccga tgcgcgcggc gcgcacgcgc
     2041 ttcggcggcg tggccaccgc gctgatccag caatggggcg aggacgacta ccgcgtgctg
     2101 gtggacgtga gccttgcgca gaccgtcgcc gcgtggctgc gccaggctgc cgcgttttcg
     2161 cgggaattgt cgtgagcaaa ccgctgttca tgtcgccgga gcacgtgcgc ctgatgaatg
     2221 agcggctcgc cggcgacgtg gcgagcaagt cagcctgcgc cgcgctgccg cgccgctatc
     2281 agctggccta tcgcctggaa gacggcgaac gcgtggtgtg gtggaccacg gtgttcgatc
     2341 cggccggcgg cgtgcgcttc cacctggcgc cgcccgaggg tgacgccgac ctgcagttca
     2401 gcggctcgta ctgggccatg atcgaattca cgcagcgttc gaagcagggc gatgcgggca
     2461 gctttccgct caccaccggc ggcgacctca acacctacgc catcatcatg ccggcattcg
     2521 cggcggcgca ggcggcggcc acgctcgaca cggaatttcc tgcgcgctga tcgccgccgg
     2581 cggcctggtg cgtgcgcgca ggcatccacc gcaccgccat acttgatgtt attaaatatt
     2641 gtctgaaaat aaaataagac tatatttaat aacatccgca gacggggtgc ggtggcggtt
     2701 tttttgcgcg tgcgatcagt tggcgcgctt gatgaactcg ccgccgcgca tgatgtgggt
     2761 gaggtgcttg ccgttggacg ccagcacgcc gatgtcgtcc agcgggttgc cgtcgaccac
     2821 cagcaggtcg gccagcgcgc cggccttgac cacgcccagc ttgtccttca tctgcacgat
     2881 gtcggcgttg accgaggtgg ccgagatcag gatgtccgcc gcggacaatg cctgccggcg
     2941 cagcgtgaat tcggtgccct gcagcacgta ctggctgccg agcaggtcgg tgccgaagcc
     3001 catgcgcacg ccggcgcgct tcatgatcga caggccggcg acggccttgt cggccacccg
     3061 ctgcagcttc tcgtaggacg agccgagcag gccgagcttc gggccatcgg ccagcagcgc
     3121 aaagatggtg gccatggtcg gcaccacgaa cgcgcccttc tcggccacga aatcggcggt
     3181 cggctggtcg atcagcgtgc cgtgctcgat gcagcgcacg ccgcattcga cgcagcggcg
     3241 cacggccgcg gcggggtggc agtgcgcgca ggcgtacgag ccgcggcggt cgcattcatc
     3301 gacgatcgcg cgcagctcgt cggtggagta ctgcgggtat tccagcggat cggtcggtga
     3361 caccacgccg cccgaggcca tgatcttgat gtggtgcgcg cccttgcgga actggtcgcg
     3421 caccgcggtg cggcaggcgt cgacgccatc ggcgatgagg cccaccatgc tgctgccgga
     3481 acagcacggg cacaggtcct cggtgccggg cgcgcgcatg tcgccgtgcc cgccggtctg
     3541 cgacagcgcg gggccgccgt agaaatagcg cgggccctgc aggtagccgt cgcgcagcgc
     3601 catggccagg ccctggtcgc cgccggccac gtcgcgcacg gcggtgaagc cgcagcccag
     3661 gatgtggctg agaaaacgcg cggcgtgatg cgcgcggtag ctcatcggcg cttccggcgg
     3721 cgacagcttg agcgagttgg catagacgtg gacgtgcgcg tcgatcaggc ccggcatcag
     3781 cgtgcggccg gtgcagtcga tgatgtcggc gccttcgccc ggcgcgtggc cgggggtcac
     3841 gcgctcgatc acgccgtcgg ctatcaccac cgtcgatggc gcggagcgca ccgggttttc
     3901 gccgtcgaac acctgcgcgt tgatgaaggc ggtcttgcgg gatgtcatgt ctgtttcctc
     3961 gtccgtcggc ggggtgtggc ccgtgttccg ggtgctgtgc gggcctgcac ggattctgcc
     4021 agcttgcccg cgcagcagcc ggtgtccgct gcgatcaaca atacagaatt gtttattgat
     4081 gaggcaattc cggggatgcc gcccgtggcg gcagccagcg gttccgccaa tgccgcgatt
     4141 cacttgcaag atacagtaat gtcttgtagc ttcgaggcca cgtgcggatg ggctcctgcc
     4201 gcatggacca gaggcgggtg atgagcacat cgatgcgtcg ccgggcgctg cctgtactgc
     4261 tgctgctgtt cggtggccgc gcgctggcgc aggacgcggg cgggcaggtg gatgcgtcgg
     4321 cgcctgcggc gcaggcggag gcgccggctg cgccagatgc aaacgccgcc gccgatacgg
     4381 ccgctccggt cgccaccatc ccggtcaaca gcaagtccac cgctgcgccc gcggccgccc
     4441 cggccagcga cgacggcagc acgcggctgg aagacgtggt ggtcacggcc accaagcggc
     4501 gcgaggcctc gcgcgaggtg ccgatcagca tcaacgcctt ccgcggcgaa gacctggaga
     4561 agcgcggcgc caagggactg gaggagatcc tgcagttcgc gcccggcgta tcgatcaaca
     4621 agaacaccag cgccgacaac aactatgtgt ccatccgcgg cgtctcgacc aattccaaca
     4681 acaatgtcta cacgcgcccc accggcctgt tcctggaaga cgcttcgctg agca
//
LOCUS       DASZZC010000381         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_860620,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000381 DASZZC010000000
VERSION     DASZZC010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860620"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..973)
                     /gene="rsmA"
                     /locus_tag="VHE37_08185"
     CDS             complement(176..973)
                     /gene="rsmA"
                     /locus_tag="VHE37_08185"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HVT35547.1"
                     /translation="MSVPHQARKRFGQNFLHDPGVIARIVKAIDPQPGDDLVEIGPGL
                     GALTAPLLARLGRMRVVELDRDVIPHLRAACGDAPGLEVIQADALTVDFGALAGSGKL
                     RVVGNLPYNISTPLLFHLLDYAPQIRDMHFMLQKEVVQRICAGAGEPDYGRLSVSLAA
                     RCDAAHLFNVGPGAFRPAPQVDSAIVRLVPRPPPVPIISWRNFDRVVSAAFSQRRKTL
                     TNSLRGLLDAAQIRTAGVDPSLRAERLDVPAYARLAELLPAQPAGVA"
     gene            complement(970..1968)
                     /gene="pdxA"
                     /locus_tag="VHE37_08190"
     CDS             complement(970..1968)
                     /gene="pdxA"
                     /locus_tag="VHE37_08190"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HVT35548.1"
                     /translation="MPTRPNRRDVRRLLITPGEPAGIGPDLAVALARERHATEIVAVA
                     DPMLLAERAVRLGLPLKLEIIDLHDAPRAPRRGTLRVLPVALAAPVRPGRLDPRNAGY
                     VLDTLRIAARACLRGQAHALVTGPVQKSVINDAGIAFSGHTEFLARLTRARMPVMMLV
                     AGSFRVALATTHLALKDVPRAITRARLTAVLRVLHHDLRARFRIARPRILVCGLNPHA
                     GEGGHLGREEIRVIEPVLRALRRGGMDLSGPLPADTLFTPARLQGADAVLAMYHDQGL
                     PVLKYAGFGEAVNVTLGLPIIRTSVDHGTALDLAGSGKADPGSLLAAVAMAQQLAA"
BASE COUNT          296 a          740 c          741 g          271 t
ORIGIN      
        1 tcgccggtgt ccagcgtcac gtactcgacc acgtgcacca ccgacagctg cgcgccgaag
       61 cccttggcca gttcctgcgc gcgccgcagc acgcgctcgc cgccggcatc cagttccact
      121 gccgccaaca cgtgcgagta atagcccatg gaccttctcc gtcagcgcgc cggcctcatg
      181 ctactccggc cggctgcgca gggagaagtt ccgccagccg ggcataggcc ggcacgtcga
      241 gccgctcggc gcgcagggag ggatcgaccc cggcggtgcg gatctgcgcc gcgtcgagca
      301 ggccgcgcag cgagttggtc agcgtcttgc gcctttgcga aaacgcggcg ctgaccacgc
      361 ggtcgaaatt gcgccacgag atgatcggca ccggcggcgg ccgcggcacc agccgcacga
      421 tggcggaatc cacctgcggc gccgggcgga acgcgccggg gccgacgttg aacaggtgcg
      481 cggcatcgca gcgcgcggcc agcgacaccg acagcctgcc gtaatccggc tcgccagcac
      541 cggcgcagat gcgctgcacc acttccttct gcaacatgaa gtgcatgtcg cggatctgcg
      601 gcgcgtaatc cagcagatga aacagcagcg gcgtggagat gttgtacggc aggttgccga
      661 ccacgcgcag cttgccgctg ccggccagtg cgccgaagtc cacggtgagc gcatcggcct
      721 ggatcacctc cagcccgggc gcgtcgccgc aggccgcgcg caggtgcgga atcacgtcgc
      781 ggtccagttc caccacgcgc atgcgcccca gccgcgccag cagcggtgcg gtgagcgcgc
      841 ccaggccggg gccgatctcc accaggtcat cgcccggctg cgggtcgatg gccttgacga
      901 tgcgcgcgat gacgccgggg tcgtgcagga agttctggcc gaagcgcttg cgcgcctgat
      961 gcgggacgct catgcggcga gctgctgcgc catcgccacg gccgctagca ggctgccggg
     1021 atcggccttg ccgctgccgg ccaggtccag cgcggtgccg tgatcgaccg aggtgcggat
     1081 gatgggcagg cccagcgtca cgttcaccgc ctcgccgaag ccggcgtact tgagcaccgg
     1141 caggccctgg tcgtgataca tggccagcac ggcgtcggcg ccttgcaggc gcgccggcgt
     1201 gaacagcgtg tcggccggca gcgggccgct caggtccatg ccgccgcggc gcagcgcgcg
     1261 cagcaccggc tcgatcacgc ggatctcctc gcgtcccaga tggccgccct cgcccgcgtg
     1321 cggattgagc ccgcacacca ggatgcgcgg ccgcgcgatg cgaaagcgcg cgcgcaggtc
     1381 atggtgcagc acgcgcagca ccgcggtgag ccgcgcgcgc gtgatggcgc gcggcacgtc
     1441 cttgagcgcg agatgggtgg tggccagtgc cacgcggaag ctgccggcga ccagcatcat
     1501 caccggcatg cgcgcgcgcg tcagccgcgc gaggaattcg gtgtggccgg agaaggcgat
     1561 gccggcatcg ttgatcacgc tcttctgcac cggcccggtg accagcgcgt gcgcctggcc
     1621 gcgcaggcag gcgcgcgcag cgatgcgcag cgtgtcgagc acgtagccgg cgttgcgcgg
     1681 gtccagccgg ccgggccgca ccggcgccgc cagcgccacc ggcagcacgc gcagcgtgcc
     1741 gcggcgcggt gcgcgcggtg cgtcgtgcag gtcgatgatc tccagcttca gcggcaggcc
     1801 gagccgcacg gcgcgctcgg ccagcagcat ggggtcggct actgctacga tttccgtagc
     1861 atgacgctct cgcgccagcg ccaccgccag gtcggggccg atgccggccg gctcgcccgg
     1921 cgtgatcagc aggcggcgga cgtcacgacg atttggccgc gtcggcatcg gacttgaggc
     1981 ggtactccac gtaggcttcc tcgcgcagcc ggcgcagcca gctgtcgtac tcgtcggccg
     2041 cgcggcgc
//
LOCUS       DASZZC010000382         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_935714,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000382 DASZZC010000000
VERSION     DASZZC010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935714"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VHE37_08195"
     CDS             <1..1008
                     /locus_tag="VHE37_08195"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVT35549.1"
                     /translation="RIAAGVLAATLATAAQAQIDLNKLRQQLNRDMQAWAAALAQDFA
                     AWRQTVAQASGNYLIPTPGTTSAEGYIQWQGAARRYLVILPQVPVANAPAILLLHPRN
                     LAPEKMGNLTRAGRLAANDGAVVLLPEAQNGAWNEDPAGSVSADDVGFLSALIDYSVA
                     HYGVDKKRVYATGYSSGGFMAARLGCQASGKLAGVGEVAATMRASLEKICVTTHALPV
                     ALIDGTSDAVVPYNGEVHALDSAPATAALWAAKAGCNATAPTTHLPNKSADDGTTVDL
                     ARYRNCGNAGEVRLYTVNQGGHTWPSSPYGVYTLALGRTTRDIDATLELWNFLSAYRA
                     P"
     gene            complement(1013..2596)
                     /locus_tag="VHE37_08200"
     CDS             complement(1013..2596)
                     /locus_tag="VHE37_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT35550.1"
                     /translation="MNYKQAGLLGGCLLPIAALAAPVPGSNCNVLPADNVWNTDISAL
                     PVHAQSAAWLASMSASSKNLHPDFGAKPYGFPFIVVPGTHSKVSVSFTYADQSDPGPY
                     PLDAQTPIEGGSDAHALMIDQDACVLYELYATKWNSGKPTAGSGAIFDLASNALRPAG
                     WTSADAAGLPIFPGLVRYDEVQAGAIHHAIRFTAAHSDKSYLWPARHQAGSASNSSLP
                     PMGARFRLKADYDISGYSAQAQVILTAMKHYGMIVADNGSDWFFQGTQDSRWGDDVLN
                     GLKGVPATEFEAVDESSLMLDANSGQARQPVVLGPVAAIDPSALDFGDQTRGTHSDAR
                     TVTLTNNGDANVSVGSVTLSGPNAAAFVLSGDGCTSGTIAPHGSCPVSVSFAPGSTGQ
                     RNASLKFADNAPDSPQSVALTGNSLAPVAGISLAPASIAWGRIAVGAVSGTRAVTVSS
                     TGTAPLVIGSVALSGGNAGDFAIAADHCSGKHLANGKTCKVVLSFAPAATGARSTSLL
                     VGDNAPGNPHNVPVSGTGK"
     gene            2844..>3216
                     /locus_tag="VHE37_08205"
     CDS             2844..>3216
                     /locus_tag="VHE37_08205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVT35551.1"
                     /translation="MDHGRRLAALLAVTLLSMSCAAGAAGRRDPLQHDVSLVEPGTPA
                     LQLAEPIPGAPISSTFGWRENPVLKRERFHAGVDFAAGVGTPVHSAAPGRIVKIKRAR
                     DRGLYVVIRHADGVKTVYSHLS"
BASE COUNT          477 a         1099 c         1125 g          515 t
ORIGIN      
        1 cggattgcgg ccggcgtgct ggcggcgacg ctggcaacgg cagcgcaggc gcagatcgac
       61 ctgaacaagc tgcgccagca gctcaaccgc gacatgcagg cctgggccgc ggcactggcg
      121 caggacttcg ccgcgtggcg gcagaccgtc gcgcaggctt ccggcaatta cctgattccc
      181 actccgggca ccaccagcgc cgagggctac atccagtggc agggcgcggc gcggcgttac
      241 ctggtgatcc tgccgcaggt gcctgtcgcc aatgctccgg cgatcctgct gctgcatccg
      301 cgcaacctgg cgccggagaa gatgggcaac ctcacgcgcg ccgggcggct cgcggccaac
      361 gacggcgcgg tggtgctgct gccggaagcg cagaacggcg cgtggaacga agatccggcc
      421 ggcagcgtca gcgcggacga tgtcggattc ctctcggcat tgatcgatta ctccgtggcg
      481 cactacggcg tggacaagaa gcgcgtctat gccaccggct actccagcgg cggcttcatg
      541 gcggcgcggc tgggctgcca ggcgtcgggc aagctggccg gcgtcggcga agtggccgcc
      601 accatgcgcg cttcgctgga aaagatctgc gtcaccaccc atgctttgcc ggtcgcgctg
      661 atcgacggca cctccgatgc ggtggtgccg tacaacggcg aggtgcacgc gctggattcc
      721 gcgccggcca ccgccgccct gtgggcggcc aaggccggct gcaatgccac ggcgccgacc
      781 acgcacctgc cgaacaagtc cgccgatgac ggcaccacgg tagacctggc gcgctaccgc
      841 aactgcggca acgcgggcga agtgcgcctg tacacggtga accagggcgg ccacacctgg
      901 ccgagttcgc cgtatggcgt gtatacgctg gcgctgggcc gcaccacccg cgacatcgac
      961 gccacgctgg aactgtggaa tttcctgtcg gcgtaccgcg cgccgtaatt ttttacttgc
     1021 cggtacccga aaccggcacg ttgtgcggat tgcccggcgc attgtcgccg accagcagcg
     1081 aggtgctgcg cgcgccggtc gcggccggcg cgaagctcag caccaccttg caggtcttgc
     1141 cgttggccag gtgcttgccg ctgcagtgat cggcggcgat ggcgaaatcg ccggcattgc
     1201 cgcccgacag cgccacgctg ccgatgacca gcggcgcggt gcccgtgctg ctcaccgtca
     1261 ccgcgcgggt gccgctgacc gcgcccacgg cgatgcggcc ccaggcgatg ctcgccggcg
     1321 cgagcgagat gccggcgacg ggcgcgaggc tgttgccggt gagcgcgacg gactgcgggc
     1381 tgtccggcgc gttgtcggcg aatttcaggc tggcgttgcg ctgcccggtg gagccgggcg
     1441 cgaagctcac gctcaccggg cagctgccgt gtggcgcaat ggttccgctg gtgcagccgt
     1501 cgccgctgag cacgaacgcg gcggcgttgg ggccggacag cgtcacgctg cccacggaaa
     1561 cattggcgtc accattgttg gtcagcgtca ccgtgcgcgc atcgctgtgc gtgccgcgcg
     1621 tctggtcgcc gaagtccagc gcgctggggt cgatcgccgc caccgggccg agcaccaccg
     1681 gctggcgcgc ctggccggag ttggcgtcga gcatcaggct ggactcgtcc actgcttcga
     1741 attccgtagc aggcacgccc ttcagcccgt tgagcacgtc gtcgccccag cgcgaatcct
     1801 gcgtgccctg gaagaaccag tcggagccgt tgtcggccac gatcatgccg tagtgcttca
     1861 tggcggtgag gatcacctgc gcctgcgccg agtagccgga gatgtcgtag tccgccttca
     1921 ggcggaagcg cgcgcccatc ggcggcaggc tgctgttgct ggcgctgccg gcctggtggc
     1981 gcgccggcca caggtagctc ttgtcgctgt gcgcggcggt gaagcggatg gcgtgatgga
     2041 tggcgccggc ctgcacctcg tcgtagcgca ccaggccggg aaagatcggc aggccggcgg
     2101 catcggcgga ggtccagccc gcgggccgca gcgcgttgga cgcgagatcg aagatcgcgc
     2161 cggagccggc agtcggcttg ccgctgttcc atttcgtcgc gtacagctcg tacagcacgc
     2221 aggcgtcctg gtcgatcatc agcgcgtgcg cgtcgctacc gccctcgatc ggcgtctgcg
     2281 cgtcgagcgg gtacgggccg ggatcgctct ggtcggcgta ggtgaagctc accgacacct
     2341 tgctgtgggt gccgggcacc acgatgaacg gaaagccgta aggcttggcg ccgaagtccg
     2401 ggtgcaggtt cttgctcgat gcgctcatcg aggccagcca ggcggcgctc tgcgcatgca
     2461 ccggcagcgc ggagatgtcg gtgttccaca cgttatcggc cggcagcacg ttgcaattgc
     2521 tgcccggcac cggcgcggcc agcgccgcaa tgggcaacag gcagccgccc agcagccctg
     2581 cttgcttgta gttcatcggt gctccccata cgcctgtcgg cgcgcctgaa aattatgcgt
     2641 cgcccgagct cagccggaaa acgatgtgca tcacgttcag ccccgcgctg ggcgccgctc
     2701 gtcctgaaag tttttgtcgc cggtgacgtg aattgtcggg ccggaaagtt atccaatgcc
     2761 ccatgcgcca cctgctctaa cgacgcggcg ccgcggcatc accagaatcg ccgcccattg
     2821 tttctcccca aaccaggatt gatgtggacc acggacggcg gctggcggca ttgcttgcgg
     2881 taaccctgtt gtcgatgagc tgcgcggcgg gggccgccgg ccggcgcgat ccattgcagc
     2941 acgacgtgag cctggtcgaa cccggcacgc ctgcgctgca gctggccgag ccgatccccg
     3001 gcgcgccgat cagctccacc ttcggctggc gcgaaaaccc ggtgctcaag cgcgagcgct
     3061 tccatgccgg ggttgatttc gccgccggtg tcggcacgcc ggtgcattcc gccgcgccgg
     3121 ggcgcatcgt gaagatcaag cgcgcgcgcg accgcggcct ctacgtggtg atccgccatg
     3181 ccgacggcgt gaagaccgtc tactcgcacc tgtcgt
//
LOCUS       DASZZC010000383         5592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1250130,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000383 DASZZC010000000
VERSION     DASZZC010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250130"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="mgtE"
                     /locus_tag="VHE37_08210"
     CDS             <1..1182
                     /gene="mgtE"
                     /locus_tag="VHE37_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HVT35552.1"
                     /translation="KPERRGAVLLETSDAVRRALIEHMPAEEIATVVRPLDADDIADL
                     VSGLREDIRADVLARLDRSEQAEVRSVLSFPEGSAGAVMVLDYIAVREDASLEAVHRL
                     LRRLKELPPHTNQLFVVDRANVLRGLLSLTKLLLEEPERTVGEVMMAQPIYFYTDDKM
                     DDVIGSFEKYDLLSAPVVNLHQQVVGRVIVDTVLDEVKRRAENQSLRQVGLTHGEDLY
                     GPVVRSGRNRWPWLGLNLFTSFASSRVIGIFDDVIAQFTAVATLIPIVASLGGNTGTQ
                     TMVLVIRALALDPRGPAQLRRAFFKEVFVALLNGSVWGAALGTVTFMMYGKWKLSLVI
                     YAAMVIELLVAAMAGVAIPTVLKRIGRDPVMGSSVLLTAVTDTCGFFIFLGLAAVFLL
                     Q"
     gene            1249..1833
                     /locus_tag="VHE37_08215"
     CDS             1249..1833
                     /locus_tag="VHE37_08215"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HVT35553.1"
                     /translation="MTQDKIAKLIEAGRLFHQRGWVPATGGNFSARVGPESLIITASG
                     THKGELTESDFLVADFAGRPEIPGRKASYETLLHCQLYRHHAQIGCVLHTHSVANTIL
                     SRRHKNIRLHGYELQKVLGVDTHESTLEVPVFANDQDIAALAARVDLELKERPQSVAY
                     LIAGHGLYAWGADVAQTRYRIEALEFMFECELRS"
     gene            1844..2389
                     /locus_tag="VHE37_08220"
     CDS             1844..2389
                     /locus_tag="VHE37_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HVT35554.1"
                     /translation="MTTLTIYRDSDAKTPLAHHEDFVTIQRELAAIGIEFERWQANKV
                     LSPLAEQAEVLEAYADSIERLNAKYGFKSMDVVSMHPDHPQAAGARGKFLAEHTHDDF
                     EIRFFVDGEGMFYIRKNGKVYMTRCCRGDLINLPADTTHWFDMGPRPNFKAIRLFTAP
                     DGWVGRFTGDRIAESFPAFQN"
     gene            2492..3169
                     /gene="mtnC"
                     /locus_tag="VHE37_08225"
     CDS             2492..3169
                     /gene="mtnC"
                     /locus_tag="VHE37_08225"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HVT35555.1"
                     /translation="MTIKAIITDIEGTTSSIDFVHQTLFPYAKAHLREFLRAHADDAE
                     VQRQLIDLVRMEGRTLSIEQAADVLEKWMSEDRKATPLKALQGMIWKAGYDSGELTGH
                     VYEDTPEYLERWASGGIRLYVYSSGSVEAQQLIFGNTEYGDLTPLFSGYFDTRVGGKR
                     ESASYAAILSKIGCKGDDVLFLSDIGEELDAARAAGLRTCQLLRDEKTKPSAAHPQAR
                     DFSQVQL"
     gene            complement(3322..4125)
                     /gene="cysQ"
                     /locus_tag="VHE37_08230"
     CDS             complement(3322..4125)
                     /gene="cysQ"
                     /locus_tag="VHE37_08230"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HVT35556.1"
                     /translation="MSLAVRDAALLEPVLGIAQEAGRRIMEVYGSEFAVTHKDDTSPL
                     TQADLAAHDHIVRALSALDAAIPVLSEESAEIPYAERRAWARYWLVDPLDGTKEFIKR
                     NGEFTVNIALIERGEPVLGVVYAPALELTYLGARGHGAERIKDGRRERITTRRTPARP
                     ALVVTKSHRDETLDVLLAKLPAHDAVSKGSSLKFCLVAEGAADFYPRTGPTSEWDTGA
                     GQAVAEAAGARVVRLPDFAPLAYNQKESLLNPGFLVIGEPGYGWEKHLA"
     gene            complement(4122..4742)
                     /gene="cysC"
                     /locus_tag="VHE37_08235"
     CDS             complement(4122..4742)
                     /gene="cysC"
                     /locus_tag="VHE37_08235"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HVT35557.1"
                     /translation="MTQQVATNVHWHEGEISRADRNKLLKHKGATLWFTGLSGSGKST
                     VATALESALFKRGILSYRLDGDNIRLGINKNLGFSEADRTENIRRIGEVAKLFVDTGI
                     IALTSFISPYKKDRDAVRKLHDDGKMDFIEIYVDCPLAEAEKRDPKGLYKKARAGEIK
                     NFTGISDPYEAPDRPELVLKTDQMTLEQEVEAVIKLLADRGIISAK"
     gene            4847..5518
                     /gene="yrfG"
                     /locus_tag="VHE37_08240"
     CDS             4847..5518
                     /gene="yrfG"
                     /locus_tag="VHE37_08240"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HVT35558.1"
                     /translation="MTNNVRIRWPEVEWVVLDMDGTILDLAFDNYFWRELVPARYAAA
                     RGLTLEAARAELAPKFTEVAHTLPWYCTDYWSEVTGLDMAELKREIRHHIGPLQGAEQ
                     FLQAVRASRRKLWLATNAHRDSWMLKMEQTAFAPYFDAIVCSHDYGAPKEDPRFWRAF
                     IEHRPFVPERALFVDDSLPVLHAAREFGIGQVVAIRHPDSTQPPRVVEEFAAVDRLVQ
                     LLPIS"
BASE COUNT          960 a         1828 c         1831 g          973 t
ORIGIN      
        1 aagcccgagc ggcgcggcgc ggtgctgctg gaaacctccg acgcggtgcg ccgtgcgctg
       61 atcgagcaca tgccggcgga ggagatcgcc acggtggtgc ggccgctcga cgccgacgac
      121 attgccgacc tggtgtccgg actgcgcgag gatatccgtg ccgacgtgct ggcgcggctg
      181 gaccgcagcg agcaggccga ggtgcgctcg gtcctgtcgt tcccggaagg cagcgccggc
      241 gcggtgatgg tgctcgacta catcgccgtg cgcgaggatg ccagcctgga agcggtgcac
      301 cgcctgctgc gccggctcaa ggaactgccg ccgcatacca accagctgtt cgtggtcgac
      361 cgtgccaacg tgctcagggg cctgctgtcg ctgaccaagc tgctgctcga ggaacccgag
      421 cgcaccgtgg gcgaagtgat gatggcgcag cccatctact tctacaccga cgacaagatg
      481 gacgacgtca tcggctcgtt cgagaagtac gacctgctgt cggcgccggt ggtgaacctg
      541 caccagcagg tggtgggccg cgtcatcgtc gacaccgtgc tcgacgaggt gaagcgccgc
      601 gcggaaaacc agtcgctgcg ccaggtcggc ctgacccacg gcgaagacct gtacggcccg
      661 gtggtcagga gcgggcgcaa ccgctggccg tggctgggcc tgaacctgtt cacctcgttc
      721 gcttcctcgc gcgtgatcgg catcttcgac gatgtcatcg cgcagttcac cgcggtggcc
      781 acgctgatcc ccatcgtcgc ctcgctcggc ggcaacacgg gcacgcagac catggtactg
      841 gtgatacgcg cgctggcgct ggacccgcgg ggcccggcgc agctgcgccg cgcgttcttc
      901 aaggaagtct tcgtcgccct gctcaatggc tcggtgtggg gcgcggcgct gggcaccgtc
      961 accttcatga tgtacggaaa atggaagctg tcgctggtga tctacgcggc catggtcatc
     1021 gagctgctgg tggcggcgat ggccggcgtg gccattccca ccgtcctcaa gcgcatcggc
     1081 cgcgacccgg tgatggggtc gagcgtgctg ctcaccgcag tcaccgacac ctgcggcttc
     1141 ttcatcttcc ttggcctggc ggcggtgttc ctgctgcagt aaaaattttc cgcactcgct
     1201 gcgcgtttcc ttgtctgaaa gaacttcaac cgttagagtc cgcgcgccat gacgcaagac
     1261 aagatcgcca aactcatcga agccggccgc ctgttccacc agcgtggctg ggtgcccgcc
     1321 accggcggca atttctccgc ccgtgtcggg ccggagagcc tgatcatcac cgcctcgggc
     1381 acgcacaagg gcgagctgac cgagagcgat ttcctggtcg ccgatttcgc cggccgcccg
     1441 gagattcccg ggcgcaaggc ttcgtacgaa accctgctgc attgccagtt gtaccggcac
     1501 cacgcgcaga tcggctgcgt gctgcacacg cactccgtcg ccaacaccat cctgtcgcgc
     1561 cggcacaaga acatcaggct gcacggctac gaattgcaga aggtgctggg cgtggacacc
     1621 cacgagagca cgctggaagt gccggtgttc gccaacgacc aggacatcgc cgcgctcgcc
     1681 gcgcgcgtgg acctggaact gaaggagcgc ccgcagagcg tggcgtacct catcgccggc
     1741 catggcctct acgcctgggg cgccgacgtg gcgcagacgc gctatcgcat cgaggcgctg
     1801 gaattcatgt tcgagtgcga actgcgctcg tagcggaggc cgcatgacca cgctgaccat
     1861 ctaccgcgac agcgatgcga aaacgccgct cgcgcaccac gaggatttcg tgacgatcca
     1921 gcgcgagctg gccgccatcg gcatcgagtt cgagcgctgg caggccaaca aggtgctgtc
     1981 gccgctggcc gagcaggccg aggtgctcga ggcctacgcc gattccatcg agcgcctcaa
     2041 cgcgaagtac gggttcaagt cgatggacgt ggtcagcatg catcccgacc acccccaggc
     2101 cgccggcgcg cgcggcaaat tcctggccga gcacacgcac gacgacttcg agatccgctt
     2161 cttcgtcgac ggcgaaggca tgttctacat ccgcaaaaac ggcaaggtct acatgacgcg
     2221 ttgctgccgc ggcgacctga tcaacctgcc ggccgacacc acgcactggt tcgacatggg
     2281 cccgcgaccg aacttcaagg ccatacgcct gttcacggcg cccgatggct gggtcggcag
     2341 gttcaccggc gacaggatcg ccgagagttt ccccgcattc cagaactgaa gcgtcattcc
     2401 ggccagcgag cgcagcgagc gcgagccgga atccaggccc gacgtcgctg cgagacaaca
     2461 actggattcc cgccgtcgcg ggaatgacga agtgaccatc aaggccatca tcacggacat
     2521 cgaaggcacc acgtcttcga tcgacttcgt ccaccagacg ctgtttccgt acgcgaaggc
     2581 gcacctgcgc gagttcctgc gcgcgcacgc ggatgatgca gaagtgcagc gccagctgat
     2641 tgacctggtg cgcatggaag gacgcacgct ttcaatcgag caggccgccg acgtgctgga
     2701 aaaatggatg tcggaagacc gcaaggccac gccgctcaag gcgctgcagg gcatgatctg
     2761 gaaagcgggc tacgacagcg gcgaactcac cgggcatgtc tacgaggaca cgccggaata
     2821 tctcgagcgc tgggccagcg gcggcatcag gctctacgtg tattcctcgg gctcggtcga
     2881 ggcgcagcag ctgatcttcg gcaacaccga gtacggcgat ctcacgccgc tgttctccgg
     2941 ctacttcgac acgcgcgtcg gcggcaagcg cgagagcgcg tcgtacgcgg cgatcctcag
     3001 caagatcggc tgcaagggcg acgacgtact gttcctgtcc gacattggag aagagctcga
     3061 cgcggcgcgc gcggccggcc tgcgcacctg tcagttgctg cgcgacgaga aaaccaaacc
     3121 atcggccgca catccgcagg cgcgcgattt ctcacaggtg cagctgtagg agaagtgcgc
     3181 cgtgcgcgcg atcggagtcc tgatcgcgca aaaaggcacc caccgcaccc cggagttgcg
     3241 tgttataaaa aatagtctaa agttaatatt agactataaa aaataacagc gaaatgcccg
     3301 gtgcggtgca gcgtttttgg ctcaagccaa gtgcttttcc cagccgtagc ccggttcgcc
     3361 gatgaccagg aagccgggat tgagcagcga ctctttctgg ttgtatgcca gcggcgcaaa
     3421 gtcgggcagg cgcacgacgc gcgcgccggc ggcttcggcc acggcctgtc ctgcgccggt
     3481 gtcccactcc gaggtcggcc cggtgcgtgg ataaaaatcc gccgcgcctt cggcgaccag
     3541 acagaacttg agcgaactgc ccttgctcac cgcgtcgtgc gcgggcagtt tggccagcag
     3601 cacgtccagc gtctcgtcgc gatgcgactt ggtcaccacc agcgccggac gtgcgggcgt
     3661 cctgcgcgtg gtgatgcgct cgcgcctgcc gtccttgatg cgctcggcgc catggccgcg
     3721 cgcgccgagg taggtgagtt ccagcgccgg agcgtagacc acgcccagca ccggctcgcc
     3781 gcgctcgatc agcgcgatgt tcacggtgaa ctcgccgttg cgcttgatga attccttggt
     3841 gccgtcgagc ggatcgacca gccagtaacg cgcccaggcg cgccgctcgg cgtaaggaat
     3901 ctccgccgat tcttccgaca gcaccgggat cgcggcatcg agcgccgaca gcgcgcgcac
     3961 gatgtgatcg tgcgcggcca ggtcggcttg cgtcagcggc gaggtgtcgt ccttgtgcgt
     4021 gacggcaaac tcggagccgt acacttccat gatgcgccgg ccggcctcct gcgcaatgcc
     4081 cagcaccggc tccagcagcg cggcgtcgcg cacggcgaga ctcatttcgc ggagatgatg
     4141 ccgcggtcgg ccagcagctt gatcaccgcc tctacttcct gctccagcgt catctggtcg
     4201 gtcttcagca ccagctccgg acggtccggc gcttcatacg gatcggaaat gccggtgaag
     4261 ttcttgatct cgccggcgcg cgccttcttg tacaggccct tgggatcgcg cttctcggct
     4321 tcggccagcg ggcagtcgac gtagatctcg atgaaatcca tcttgccgtc gtcgtgcagc
     4381 ttgcgcaccg cgtcgcggtc cttcttgtac gggctgatga agctggtcag cgcgatgatg
     4441 ccggtgtcga cgaacagctt ggccacttcg ccgatgcggc ggatgttctc ggtgcgatcg
     4501 gcctccgaga agcccaggtt cttgttgatg cccaggcgga tgttgtcgcc gtcgaggcgg
     4561 tacgacagga tgccgcgctt gaacagcgcc gactccagcg cggtggccac ggtgctcttg
     4621 ccgctgccgg acaggccggt gaaccagagc gtggcgccct tgtgcttgag gagcttgttg
     4681 cggtcggcgc gggagatttc gccttcgtgc cagtgcacgt tcgttgcgac ttgctgggtc
     4741 atgatctaat tccgtcggaa gtgaacgacg cggctgcatg ccgcggcggc gcgaatgata
     4801 agccaacttg caggcccgga ccgtggtccg atcgagtgag cctttcttga ccaacaacgt
     4861 gcgcatccgc tggcccgagg tggaatgggt ggtgctggac atggacggca ccatcctcga
     4921 ccttgctttc gacaactact tctggcgcga gctggtgccg gcgcgctatg ccgccgcgcg
     4981 cgggctgacg ctcgaagccg cgcgcgccga gctggccccg aaattcaccg aggtggcgca
     5041 cacgctgccc tggtactgca ccgattactg gagcgaggtc accggcctgg acatggccga
     5101 gctcaagcgt gagatccgcc accacatcgg cccgctgcag ggcgccgagc aattcctgca
     5161 ggcggtgcgg gcttctcggc gcaagctgtg gctggccacc aacgcgcacc gcgacagctg
     5221 gatgctgaag atggagcaga ccgcgtttgc gccgtatttc gatgcgatcg tgtgctcgca
     5281 cgattacggc gcgcccaagg aagacccgcg tttctggcgc gcgttcattg agcaccgtcc
     5341 gttcgtgcct gagcgcgcgc tgttcgtgga cgattcgctg ccggtgctgc acgcagcgcg
     5401 cgagttcggc atcggccagg tggtggccat ccgccatccg gattccaccc agccgccgcg
     5461 cgtggtggag gagttcgccg cggtggaccg gctggtgcag ctgctgccga tcagttgagc
     5521 gctacttgcg ctctgcccgc gccttggcga tgaggaagtc ggcgatcttg aacatgtccg
     5581 acggcgggtt ct
//
LOCUS       DASZZC010000384         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_800009,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000384 DASZZC010000000
VERSION     DASZZC010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800009"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VHE37_08245"
     CDS             <1..603
                     /locus_tag="VHE37_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HVT35559.1"
                     /translation="KVAAIEAHWQRGGDVPLLLFAVPDMERARNDFEIAVPYLGSLIL
                     THSLHGEVPGLTDVPPEDRPPVIVVFWSFRIMVGLALIMLAVAWLGVLLLARTRGSPP
                     RWFLRIACACGPVGFLAVLAGWITTEVGRQPWLVYGLLRTADGASQVPAGNVEFSLAL
                     FIGVYALLFYAFGVFFARAVRRGPGQPPPGNIAQVAEAHS"
     gene            600..1616
                     /locus_tag="VHE37_08250"
     CDS             600..1616
                     /locus_tag="VHE37_08250"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HVT35560.1"
                     /translation="MNQSHDALPLVWMLFMAASISLYVMLDGFTLGIGILFPFARGAS
                     ERDRMMASVSPVWDGNQTWLVSGGMALFAAFPAAYHLLLNALYIPVLVMVIALVFRGV
                     AFEFRVRDNWRWNAAFIGGSTAAALCQGLILGTYVLGFEYDGKTLVTRPLDFISPFSC
                     VVAASLVLDYALLGACWLAWKSEGELRAWALAQARRLLLPVAAGVGGICIWMPLQVPA
                     IWERWFSWPNMIFLSPVPIWSTVLLAILWRLLTRKNLTADVLPFLCCIGLYLLALTGL
                     AVSLWPYVVPRALTFWDVAAPEESLLFTMVGVFLITPVILVYTAHAYWVFRGKVADDA
                     GYHH"
     gene            1741..2181
                     /locus_tag="VHE37_08255"
     CDS             1741..2181
                     /locus_tag="VHE37_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35561.1"
                     /translation="MKRMLLTLAAGALAVAAVPALAQVGVAVNIGEPGFYGQIDIGQA
                     PPPQVIYSSPVVVEQEVAPAMPIYLHVPPGYAKHWRQHCREYNACNRRVYFVRDDWYQ
                     KVYVPHYRQNHEFYEGGRGRIEEHEHEHDWHHDHDHDHDHDHDH"
     gene            2304..>3653
                     /locus_tag="VHE37_08260"
     CDS             2304..>3653
                     /locus_tag="VHE37_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HVT35562.1"
                     /translation="MRNILIIGAGFCGTMAAVQLLRQPPPGPIRIILLNRSGLMARGV
                     AYGTRTERHVLNVPAARMSALPGHEDDFLRYAQQHDARITGASFVPRQLYGQYLEHLL
                     TEAARAVPAGCSFRSMVGEATRIRVGAGGRSALVELDHGETIHADRVILALGNYSPAH
                     PKVDEDSRAFFRSPRYVQDAWRPEALRVARMDQPVLLIGTGLTMLDVLLDLQARGQRA
                     PVHALSRRGLLPLAHRELDTPPPPVNLLPDCLGAGGSVRRCLRALRRKVAEVTAQGGD
                     WRDVIGALRPHTPALWHQLGTVERRRFLRHLRSFWDLHRHRCAPELGAALRESVERGE
                     LRVIAGRLVAFDEQADGVDVIYRPRGARDTQRLRVSTVINCTGPEHDTRTLREPLLAH
                     LRASGLMVPDELGLGFLTDANYALRDLRGEASRVLFYAGPFLRAQHWEATAVPELRVH
                     "
BASE COUNT          484 a         1306 c         1244 g          619 t
ORIGIN      
        1 aaagtcgccg cgatcgaagc gcactggcag cgcggcggcg acgtgccgct actgctgttc
       61 gccgttcccg acatggaacg cgcgcgcaac gacttcgaga tcgccgtgcc gtacctgggc
      121 agcctgatcc tcacccacag cctgcatggc gaggtgcccg gcctcaccga cgtgccgccg
      181 gaagaccgcc cgccggtgat cgtggtgttc tggagtttcc gcatcatggt cggcctcgcg
      241 ctgatcatgc tcgcggtggc gtggctcggc gtattgctgc tcgcgcgcac gcgcggctcg
      301 ccgccgcgct ggttcctgcg catcgcctgc gcctgcgggc cggtcggttt cctcgcggtg
      361 ctcgccggct ggatcaccac cgaggtcggc cgccagccgt ggctggtcta tggcctgctg
      421 cgcacggccg acggcgcgtc gcaggtgccg gccggcaacg tcgagttctc gctggcgctg
      481 ttcatcggcg tctacgcgct gctgttctat gccttcggcg tgttcttcgc gcgcgcggtg
      541 cggcgcgggc ccggccagcc gccgccgggc aacatcgcgc aggtggcgga ggcgcattcg
      601 tgaaccagag ccacgatgcc ttgccgctgg tgtggatgct gttcatggcg gcgtcgatct
      661 cgctgtacgt gatgctcgac ggtttcacgc tgggcatcgg catcctgttc ccgttcgcgc
      721 gcggcgcctc cgagcgcgac cgcatgatgg cctcggtgtc gccagtgtgg gacggcaacc
      781 agacctggct ggtatccggc ggcatggcgc tgttcgccgc gtttcccgcc gcctatcacc
      841 tgctgctcaa tgcgctgtac atcccggtgc tggtgatggt gatcgcgctg gtgttccgcg
      901 gcgtggcctt cgaattccgc gtgcgcgaca actggcgctg gaacgccgcg ttcattggcg
      961 gctcgacggc cgccgcgctg tgccagggat tgatcctggg cacttatgtg ctcggcttcg
     1021 agtacgacgg caagacgctg gtcacgcgcc cgctggattt catctcgccg ttctcctgcg
     1081 tggtcgccgc aagcctggtg ctcgactacg cgctgcttgg cgcctgctgg ctcgcatgga
     1141 agtccgaggg cgagttgcgc gcgtgggcgc tggcgcaggc gcgccgattg ctgctgccgg
     1201 tagccgccgg cgtcggcggc atctgcatct ggatgccgct gcaggtgccg gccatctggg
     1261 agcgctggtt ctcctggccc aacatgattt ttctttcgcc ggttccgatc tggagcacgg
     1321 tgctgctggc catcctgtgg cgcctgctca cgcggaaaaa ccttaccgcc gatgtcctgc
     1381 ccttcctctg ctgcatcggc ctctacctgc tggcgctcac cgggctggcg gtgagcctgt
     1441 ggccctacgt ggtgccgcgc gcgctcacct tctgggacgt ggccgcgccg gaggaatcgc
     1501 tgctgttcac catggtcggc gtgttcctga tcacgccggt gatcctggtc tacaccgcgc
     1561 acgcgtactg ggtgttccgc ggcaaggtgg ccgacgacgc tggctatcac cactgagcgc
     1621 gaaccgggac gccatccgct gcgtctaacc acgggtttgc aagttaagaa gagcaacgcg
     1681 gtcagcggcg attcatccca ccgccgcagg ctgtgcccac gtccctcaca ggagtacgtc
     1741 atgaaacgca tgctgctcac tctcgcagcc ggtgcgctgg cggttgccgc cgtgcccgcg
     1801 ctggcgcagg tcggcgtcgc cgtgaatatc ggcgagccgg gtttctacgg ccagatcgac
     1861 atcggccagg cgccgccgcc gcaggtgatc tacagctcgc cggtggtggt ggaacaggaa
     1921 gtggcgccgg ccatgcccat ctacctgcac gtgccgccgg gttatgccaa gcactggcgc
     1981 cagcactgcc gcgagtacaa cgcgtgcaac cgccgcgtgt acttcgtgcg cgacgactgg
     2041 taccagaagg tctacgtgcc gcactaccgg cagaaccacg agttctacga aggcggccgc
     2101 ggccgcatcg aggaacacga gcacgagcac gactggcacc acgaccacga tcacgaccac
     2161 gatcacgatc acgatcattg agcgcacgtt ccgccgcgcc ggcgtgagca agatcacgcc
     2221 ggcgcggcgg gcgtgagtgt tttgcatggt gcggcggcac gcggcgcgct acattggcgc
     2281 gaacttcgca agcggtttcc gccatgcgca acatcctcat catcggcgcc ggcttctgcg
     2341 gcaccatggc cgcagtgcag ctgctgcgcc agccgccgcc cgggcccatc cgcatcatcc
     2401 tgctcaaccg ctccggcctg atggcgcgcg gcgtcgccta cggcacgcgc accgagcgcc
     2461 acgtgctcaa cgtgccggcg gcgcgcatga gcgcgctgcc cggacacgaa gatgatttcc
     2521 tgcgctatgc gcagcagcac gacgcgcgca tcaccggcgc ctcgttcgtg ccgcgccagc
     2581 tgtacggcca atacctcgag cacctgctga ccgaggcggc acgcgcggtg ccggccggct
     2641 gctcgttccg ctccatggtg ggcgaagcca cgcgcatccg cgtcggcgcc ggcggccgct
     2701 cggcgctggt cgagctcgat cacggcgaga ccatccacgc cgaccgcgtg atcctggcgc
     2761 tgggcaacta ctcgccggcg catccgaaag tcgatgagga ctcgcgcgcg tttttccgca
     2821 gcccgcgcta tgtgcaggat gcgtggcggc cggaagcgct gcgcgtggcg cgcatggacc
     2881 agccagtgct gctgatcggc accggcctga ccatgctcga cgtgctgctc gacctgcagg
     2941 cgcgcggcca gcgcgcgccg gtccatgcgc tgtctcgccg aggcctgctg ccgctggcgc
     3001 accgcgagct cgacacgccg ccgccgccgg tgaacctgct gcccgactgc ctgggcgccg
     3061 gcggctcggt ccgccgctgc ctgcgcgcgc tgcggcgcaa ggtggcggaa gtcactgcgc
     3121 agggcggcga ctggcgcgac gtgatcggcg cgctgcgccc gcacacgccg gcgctgtggc
     3181 accagctggg cacggtggaa cggcgccgct tcctgcgcca cctgcgcagc ttctgggatc
     3241 tgcaccggca ccgctgcgcg cccgaactcg gcgcagcact gcgcgaatcg gtggagcgcg
     3301 gcgagctgcg cgtcatcgcc ggccgcctgg tcgccttcga tgaacaggcc gacggcgtcg
     3361 acgtgatcta ccgcccgcgc ggcgcgcgcg acacgcagcg cctgcgcgtg tccaccgtga
     3421 tcaactgcac cgggcccgag cacgacacgc gcacgctgcg cgaaccgctg ctggcgcacc
     3481 tgcgcgccag cgggctgatg gtgccggacg aactcgggct gggcttcctc accgacgcca
     3541 actacgcgct gcgcgacctg cgcggcgagg cctcgcgcgt gctgttctac gccggcccgt
     3601 tcctgcgcgc gcagcactgg gaagccaccg cggtacccga gctgcgcgtg cac
//
LOCUS       DASZZC010000385         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_92692,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000385 DASZZC010000000
VERSION     DASZZC010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92692"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VHE37_08265"
     CDS             complement(<1..690)
                     /locus_tag="VHE37_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HVT35563.1"
                     /translation="MIGRFLAKLVALVVLLLVALGLLLRARYGGGDDFPDRHTGPARL
                     PESALESMADLPTPPGNIAVSADGRVFFSLHPEAHPALKVVELKNGKLIPYPSEAYQN
                     GSDAHAFDNVLGIRIDAQNRLWTLDNGSHGLHPVRLLAFDLATGRSAHEYTFDPSIAG
                     LGSHFNDLQVSPDGKFIYIADASFFGKTPAIVVYDVEHRSARRVLERHPAVTAEPYTP
                     VVQGRRMQILGM"
     gene            complement(687..890)
                     /locus_tag="VHE37_08270"
     CDS             complement(687..890)
                     /locus_tag="VHE37_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35564.1"
                     /translation="MLRIIVTNPEEVARRRSPLANFAAAIAPDFIRQKVDEGIAERLI
                     EQLAAEGVRARVLIEPNPPDADA"
     gene            complement(883..1590)
                     /gene="rnt"
                     /locus_tag="VHE37_08275"
     CDS             complement(883..1590)
                     /gene="rnt"
                     /locus_tag="VHE37_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HVT35565.1"
                     /translation="MQNSSPPALPKPKIGRRFRGFLPVVIDIETGGFNSQTDAVLEIA
                     AVILGMDEAGNLVRRQTVFSHVTPFPGANIEKASLEITGIRLDSPLRLAQPENEALAH
                     LFKPIRQAVSENGCTRAIMVGHNAHFDLGFLNAAVARAGIKRNPFHPFSVLDTATLAG
                     AALGQTVLAKALLAARLPYDTAAAHSAIYDAECTADLFCLLLNRMRPIYDSFVSATDE
                     VETPDTPDAQEPVAGDA"
     gene            complement(1628..>1972)
                     /locus_tag="VHE37_08280"
     CDS             complement(1628..>1972)
                     /locus_tag="VHE37_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35566.1"
                     /translation="IYDPSVGKTDEADNLLATLAQHGMELPPARREHAILRASGRVQY
                     IPADIELAKKAALDLSRLEGLVPSIQSAYVIGWAMDEATRMAPEQVIVVMLTESAERD
                     LLRMNQALGDLT"
BASE COUNT          297 a          636 c          707 g          332 t
ORIGIN      
        1 catgcccagg atctgcatgc gccggccctg caccaccggc gtgtacggct cggcggtgac
       61 cgccgggtgg cgttcgagca cgcggcgtgc gctgcggtgc tcgacgtcgt acaccacgat
      121 ggccggcgtc ttgccgaaga agctggcatc ggcgatgtag atgaacttgc cgtcgggcga
      181 cacctgcaga tcgttgaagt gcgagccgag acccgcaatg gagggatcga aggtgtactc
      241 gtgcgcgctg cgcccggtgg cgaggtcgaa tgccagcagg cgcaccgggt gcaggccgtg
      301 gctgccgttg tccagcgtcc acagccggtt ctgtgcgtcg atgcggatgc ccagcacgtt
      361 gtcgaaggcg tgcgcgtcgc tgccgttctg gtaggcctcg ctcggatacg ggatcagctt
      421 gccgttcttc agttccacca ctttcagcgc cgggtgcgct tcggggtgca gcgagaagaa
      481 cacgcggcca tcggccgaca ccgcgatgtt gcccggcggc gtcggcaggt cggccatgct
      541 ttccagcgcc gattccggca gccgcgcggg gccggtgtgg cggtcgggaa aatcgtcgcc
      601 gccgccgtag cgggcgcgca gcagcaggcc gagcgccacc agcagcaaca ccaccagcgc
      661 gaccagcttg gccaggaaac ggccgatcat gcgtccgcgt ccggcgggtt gggttcgatc
      721 agcacgcgcg cgcgcacccc ttcggccgcc agctgttcga tcaaccgctc ggcgatgcct
      781 tcgtcgacct tctggcggat gaagtccggc gcaatggcgg cggcaaagtt ggccagcggg
      841 gaacgcctgc gcgccacttc ctcgggattg gtgacgatga ttctaagcat cgccggccac
      901 cggctcctgt gcatcgggtg tatctggcgt ttccacctcg tccgttgccg agacgaaact
      961 gtcgtagatc gggcgcatgc ggttgagcag caggcagaac aggtcggcgg tgcattcggc
     1021 gtcgtagatg gccgagtgcg cggcggcggt gtcgtacggc agcctggcgg cgagcagcgc
     1081 cttggccagc accgtctggc cgagcgcggc gccggccagc gtggcggtgt cgagcaccga
     1141 gaatgggtgg aacggattgc gcttgatgcc ggcgcgggcg accgccgcgt tgagaaatcc
     1201 caggtcgaaa tgcgcgttgt ggccgaccat gatcgcacgc gtgcagccgt tctcggacac
     1261 cgcctggcgg atgggcttga acaggtgcgc cagcgcctcg ttctccggct gcgccagccg
     1321 cagcgggctg tcgaggcgga taccggtgat ctccagcgag gccttctcga tgttggcccc
     1381 agggaagggc gtgacatgac tgaacacggt ctgccggcgc accaggttgc cggcttcgtc
     1441 catgcccagg atcaccgcgg cgatctccag caccgcgtcg gtctgcgaat tgaagccgcc
     1501 ggtttcgatg tcgatgacca ccggcaggaa gccgcggaag cggcggccga tcttgggctt
     1561 gggaagggct gggggagagg aattttgcat gccgccggca tgatacccgc aacgccgcag
     1621 gcgacgctca ggtcaggtcg ccgagcgcct ggttcatgcg cagcaggtcg cgctccgccg
     1681 attcggtgag catcaccacg atcacctgct cgggcgccat gcgcgtggct tcgtccatgg
     1741 cccagccgat gacgtaggcg ctctggatcg acggcaccag gccttccagc cgcgacaggt
     1801 cgagcgccgc cttcttcgcc agctcgatgt cggcgggaat gtattgcacg cggccgctgg
     1861 cgcgcaggat ggcgtgctcg cgccgcgccg gcggcagttc catgccgtgc tgcgccagcg
     1921 tggccagcag attgtcggcc tcgtcggtct tgcccaccga ggggtcgtag at
//
LOCUS       DASZZC010000386         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_407898,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000386 DASZZC010000000
VERSION     DASZZC010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407898"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2175)
                     /locus_tag="VHE37_08285"
     CDS             complement(<1..>2175)
                     /locus_tag="VHE37_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HVT35567.1"
                     /translation="RVLRDPVSSAWLWVEYHQPLGTYDSYITQQSSSNLYQGALVRYE
                     NQLQSGYVYLLDFSPSATPNNFLDSDLTPGHSWSDPYSLLTLSVTHADASGLAVTVGY
                     DAPCATLALSQDAYSSTAQSGSVTVTAPADCAWQASTAAGWISFTGATSGMGNGVVTF
                     NLAANSDATQRNSYITVQRQSLPLAQAGSNGLLVHPLLPAFGSGSVATFTTLIDDGAG
                     TSSDQESFWLDDGSGSPLCKFDLAGSKLRLDGSLLIDPGSANSISNGNCTVYGGESAL
                     ADSGTQRRATWRVAFGATLSGAVRVQMDAWHAGTFAFNQSVGTWAITPSPAIAAVQFP
                     AAGQGDQVPVTIFGNFTHFSAASTLKFSGSGIAVSNVTAPSPTELDATLTIAAHAAVG
                     ARTLTVTTGAESVSEPYTVHAPEPPAAPGNVHATPGDSKMVLGWAPVYGATGYNIYLG
                     TAAGGESDTPVLANVSGTTATVTGLVNGTPYYFIVRAYNASYTGAASVEISATPGALA
                     APHLSYSRAGDGAIYLSWNGVTGAASYNVYQGTSAGGEASTPVRSGVTSTQATVPNLV
                     NGTHYFFTVRMVNVDGTGPASNELQATPVALTAPSGLTAQAGDGSATLRWTASTGADG
                     YTIYQATTPGGEGSSWITYTSATSITINGLNNGTTYYFTVRGFNFQKIGPPSNEASTI
                     PGPPVPAAPTGVSAVGGNHAVTVAWSASAGAVSYQVYRGSAAG"
BASE COUNT          344 a          710 c          758 g          363 t
ORIGIN      
        1 cccgcggcgc tgccccggta gacctggtag ctgaccgcac cggcagatgc cgaccaggcg
       61 acggtgaccg catggtttcc gcccaccgca ctcacgccgg tcggtgccgc cggcaccggc
      121 gggccgggga tggtcgatgc ctcgttggat ggcgggccga tcttctggaa attgaaaccg
      181 cgcacggtga agtagtacgt ggtgccgttg ttcagcccgt tgatggtgat gctggtcgcg
      241 gacgtgtaag tgatccagct ggagccttcg ccgccgggcg tcgtcgcctg gtagatggtg
      301 taaccgtcgg caccggtgga cgcggtccag cgcagcgtcg cgctgccgtc gccggcctgc
      361 gccgtcaggc ccgaaggcgc ggtgagcgcc accggcgtgg cctgcagttc attcgatgcc
      421 gggccggtgc cgtcgacatt gaccatgcgc acggtgaaga agtaatgcgt gccgttgacc
      481 agattgggca cggtggcctg ggtcgacgtc acgccgctgc gcaccggcgt ggatgcttca
      541 ccgcccgcgc tggtgccctg gtacacgtta tagctcgccg ccccggtgac gccattccag
      601 gacaggtaaa tcgctccgtc cccggcacgc gagtaggaca ggtgcggcgc cgccagcgcg
      661 ccgggcgtgg cgctgatctc caccgatgca gcgccggtgt agctggcgtt gtatgcacgg
      721 acgatgaagt aatacggcgt gccattgacc aggcccgtga cggtggccgt ggtcccgctg
      781 acattggcca gcaccggcgt gtcggattcg ccacccgccg cggttcccag gtagatgttg
      841 tagccggtgg cgccatacac cggcgcccag ccgagcacca tcttcgaatc ccccggcgtc
      901 gcatgcacgt tcccgggggc cgccggcggc tccggcgcgt gcacggtgta gggttcgctg
      961 accgactcgg caccggtggt gacggtgagc gtgcgcgcgc caacggccgc gtgggcggcg
     1021 atggtcagcg tcgcatcaag ttcagtgggc gacggcgcgg tgacattcga aacggcaatg
     1081 ccactgccgc tgaacttgag cgtcgatgcc gcggagaaat gggtgaagtt cccgaagatg
     1141 gtgaccggca cctggtcgcc ctggccggca gcgggaaact gcaccgccgc aatggccggc
     1201 gaaggagtga tcgcccaggt gcccacgctc tggttgaagg cgaaggtgcc cgcgtgccag
     1261 gcatccatct gcaccctcac cgcgccgctc agggtggcgc cgaatgccac ccgccaggtg
     1321 gcgcgccgct gcgtgccgga atcggccagg gcgctttccc cgccgtacac cgtgcagttg
     1381 ccgttcgaga tcgaattggc gctcccggga tcgatcagca gtgaaccgtc gaggcggagt
     1441 ttcgagccgg caagatcgaa cttgcacagc ggcgatcccg agccatcatc cagccagaag
     1501 ctttcctggt cgctgctggt ccctgcccca tcgtcaatca gtgtggtgaa ggtcgccacg
     1561 ctgccgctgc cgaatgcggg cagcagcgga tgcaccagca agccgttgct gccggcctgc
     1621 gccagcggca ggctttgccg ctgcacggtg atgtagctgt tgcgctgcgt ggcatcgctg
     1681 ttggccgcca ggttgaaggt gaccacgcca ttgcccatgc cgctggtagc gccggtgaag
     1741 ctgatccagc ccgccgccgt cgacgcctgc caggcgcagt cggccggcgc cgtcaccgtg
     1801 accgatccac tttgcgccgt tgacgaataa gcatcctgcg acagcgccag cgtggcgcac
     1861 ggcgcgtcgt agccgaccgt gaccgcgagc ccgctggcat cggcgtgggt cacgctgagc
     1921 gtgagcagcg agtacggatc ggaccacgag tgccccggtg tgagatcgga gtcgaggaaa
     1981 ttgttcggcg tggctgaagg gctgaagtcc agcaggtaca cgtacccgga ctgcaactgg
     2041 ttttcgtaac gcaccagcgc gccctggtag aggttcgacg acgactgctg cgtgatgtag
     2101 ctgtcgtagg tacccagcgg ctggtgatac tccacccaca accacgcgct ggagaccgga
     2161 tcgcgcagca cgcgc
//
LOCUS       DASZZC010000387         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1057058,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000387 DASZZC010000000
VERSION     DASZZC010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057058"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VHE37_08290"
     CDS             <1..557
                     /locus_tag="VHE37_08290"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HVT35568.1"
                     /translation="KDNNCYHYEQQTHPIALVRWLYGSPREISDFCIVDGKLVVSHFE
                     YRNDKRDKDSFTVDFDWAAKKVTGTKRGVVTVHDLDGPTWDRFALQQVVRLWARAHAD
                     APAGTSVELNMADDNRVTAYRFAIVGHETVETAAGKFDTLRVDRVDNPNRQLRSWLAP
                     SLDYAPVKIEQVEKGDVKLRMLLE"
     gene            554..1021
                     /locus_tag="VHE37_08295"
     CDS             554..1021
                     /locus_tag="VHE37_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HVT35569.1"
                     /translation="MTGIKTYTLADLDALAVQARAHPRRRANLNLHSGNEALVHRFVI
                     ALCRDSYIRPHRHTQPHKLEMATALQGELEWLFFDDGGAITRRVRVSPAGPVFGLEVP
                     PGVWHGLIAHSGSASFLEVKQGPYDAATDKELAPWAPAEGAVEAGEYHQKLYT"
     gene            1114..2109
                     /locus_tag="VHE37_08300"
     CDS             1114..2109
                     /locus_tag="VHE37_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HVT35570.1"
                     /translation="MDDYKAEPRAPQMDIFNPKHLTPPDPILFYSRKDPLDPRIGDVV
                     SSNFDAYGSAQVVIIGCPQDDGVLRAGGRPGSVAAPAEIRRSLYRMSTAGIGDLRIQD
                     VGDIVPQNKLEATHALQQQWVQQFLTDGKRVITLGGGSDIAYPDCSALSYIAPNLAAI
                     NVDSHFDVRNDPACNSQTAFNQLLEENCVRPEALFQIAYQQHINSPADERYLRERGVN
                     MCSLKSLRELGVAPAFRHMLRSKNRFDAIFWAFSMEAVRGADAPGVSLPSPLGLTAEE
                     FCLLAEIAGRDERTRVVEFSNVNPASDIDSITSRLAAVAVHFYLAALAEALHVRN"
BASE COUNT          402 a          764 c          722 g          324 t
ORIGIN      
        1 cgaaggacaa caactgctat cactacgagc agcagacgca tcccatcgca ctggtgcgct
       61 ggctctacgg ctcgccgcgc gagatatcgg atttctgcat cgtcgacggc aagctggtgg
      121 tcagccactt cgagtaccgc aacgacaagc gcgacaagga cagcttcacc gtcgacttcg
      181 actgggccgc gaagaaggtc accggcacca agcgcggcgt ggtcacggtg catgatctcg
      241 acggccccac ctgggaccgc ttcgcgctgc agcaggtggt gcgcctgtgg gcgcgcgcgc
      301 acgccgatgc gcccgcgggc accagcgtgg aactgaacat ggccgacgac aaccgcgtga
      361 cggcgtatcg cttcgccatc gtcgggcatg agacggtgga aacggcggcg ggaaaattcg
      421 acaccttgcg cgtcgaccgc gtggacaatc ccaaccgcca gctgcgctcg tggctggcgc
      481 cgtcgctgga ctacgcgccg gtgaagatcg agcaggtgga gaagggcgac gtgaagctgc
      541 gcatgctgct cgaatgaccg gcatcaagac ctacacgctg gcggacctcg atgcgctcgc
      601 cgtgcaggcg cgcgcgcacc cgcgccgccg cgccaacctc aacctgcaca gcggcaacga
      661 ggcgctggtg caccgcttcg tcatcgcgct gtgccgcgac agctacatcc gcccgcaccg
      721 ccacacccag ccgcacaagc tggagatggc cacggcgctg cagggagagc tggaatggct
      781 gttcttcgac gatggcggtg ccatcacccg ccgcgtgcgc gtgtcgccgg ccggccccgt
      841 attcggcctg gaagtgccgc ccggcgtctg gcatggactc atcgcgcaca gcgggagtgc
      901 gtcctttctc gaggtaaaac agggcccgta cgacgccgca accgacaagg aactggcgcc
      961 ctgggcaccg gccgaggggg ccgtggaagc aggggaatac caccagaagc tctacacctg
     1021 aagtgggatg atctttgcgg cggtactggt atccttgaga catagttcac ggtcgggccc
     1081 gccaggggca ccgccggggg agaagaggtt ccagtggacg attacaaagc cgagccacgt
     1141 gcgccgcaga tggacatatt caacccgaag cacctgacgc cgccggaccc gatcctgttc
     1201 tattcgcgca aggatcctct ggacccgcgc atcggcgacg tggtgtcgtc caacttcgac
     1261 gcttacggat cggcgcaggt ggtgatcatc ggctgcccgc aggatgacgg cgtgctgcgc
     1321 gccggcggcc gtcccggctc ggtggcggcg ccggcggaaa tccgccgctc gctgtaccgc
     1381 atgtccaccg ccggcatcgg cgacctgcgc atccaggacg tgggcgacat cgtgccgcag
     1441 aacaagctgg aggccaccca cgcgctgcag cagcaatggg tgcagcagtt cctcaccgac
     1501 ggcaagcggg tgatcacgct cggcggcggc agcgacatcg cctacccgga ctgctcggcg
     1561 ctgtcgtaca tcgcgcccaa cctggcggcg atcaacgtcg actcgcactt cgatgtgcgc
     1621 aacgaccccg cgtgcaacag ccagaccgcg ttcaaccagc tgctggaaga aaactgcgtg
     1681 cggcccgagg cgctgttcca gatcgcctac cagcagcaca tcaactcgcc ggccgacgag
     1741 cgctacctgc gcgagcgcgg cgtcaacatg tgcagcctca agtcgctgcg cgaactgggc
     1801 gtggccccgg ccttccgcca catgctgcgc agcaagaacc gcttcgacgc cattttctgg
     1861 gccttcagca tggaagcggt gcgcggcgcc gatgcgcccg gcgtgtcgct gcccagcccg
     1921 ctgggcctga ccgcggaaga gttctgcctg ctggccgaga tcgccggccg cgacgagcgc
     1981 acgcgcgtgg tggagttctc caacgtcaac ccggccagcg acatcgattc gatcaccagc
     2041 cgcctggcgg ccgtcgccgt ccacttctac ctcgccgcgc tggccgaagc cttgcacgtg
     2101 cgcaactgag gagccgccga tgaacaagct gctgctgatg ctcgtcgcca cgctgacgct
     2161 cgccgcctgc ggctccagcg atccgcaggc cgccgagaag cggaagaagg aa
//
LOCUS       DASZZC010000388         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_225888,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000388 DASZZC010000000
VERSION     DASZZC010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225888"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VHE37_08305"
     CDS             complement(<1..657)
                     /locus_tag="VHE37_08305"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT35571.1"
                     /translation="MRDGPSIVRIAALIGDQARAEVLTALMADRALTASELAHVAGVT
                     KQTISAHLSKLRDAGLVAVEHQGRHRYFRLADRDVAQLLESLMGVAFRTGALRARPGP
                     REPALRKARVCYDHLAGELGVLAYERLLAHKALRHDGDALALTPRGREWFTRFGIDVD
                     RAASGKRAACRACLDWSERRQHLAGVLGGALLARIQQLGWARRARDSRVMQFSPAGER
                     A"
     gene            731..1078
                     /locus_tag="VHE37_08310"
     CDS             731..1078
                     /locus_tag="VHE37_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HVT35572.1"
                     /translation="MNITCFIRYQIDPFQRDAFRQYAHNWGRIIPRCGGQLVGYFLPH
                     EGSNDIAWGVIAFDSLAAYERYRARLQSDDQGKANFAFAQERRLILREERSFLAGVDG
                     TLNVACTIAAGAA"
     gene            1075..1422
                     /locus_tag="VHE37_08315"
     CDS             1075..1422
                     /locus_tag="VHE37_08315"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVT35573.1"
                     /translation="MIAVIFEVLPQAAQRQAYLDTAASLRPLLERIDGFISIERFESL
                     AQPGKLLSLSYWRDQAAVHQWRTLEAHRAAQAAGRAEIFADYRLRIAAVVRDYGMNDR
                     AQAPEDSRRAHHA"
     gene            complement(1426..2952)
                     /locus_tag="VHE37_08320"
     CDS             complement(1426..2952)
                     /locus_tag="VHE37_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVT35574.1"
                     /translation="MNAPITGVLRAVTILALALGTFMQVLDTTIANVSIPTIAGDLGV
                     SANQGTWIITSFAVANGISVPLTGWLMQRYGVVRVFVLSVLAFTAASLLCGIAWNISS
                     LIAFRVLQGAVSGPMIPGSQALLISLFPADKKGAALAVWSMTTLVAPICGPILGGFIS
                     DNYSWAWIFLINVPVGLFCAAISWRALAASETPTRVLPIDRIGLGLLVLWVGALQVLL
                     DQGKDLDWFNSPAIVVLAIVAAVTLAAWIIWEMTERHPIVDLSLFKSRSFTLGTLALC
                     LGYAVFFGNVVLLPLWLQTQLGYTATWAGLVAAPSGVAAVLVSPLVGRYVGRIDPRVF
                     ATMSFVLFSASYFMRAGYTADASFAVLVMPLLVQGLAMGMFFVALLTILLDGLPPEKV
                     PAASGLSNFLRITGSSFATSLATTFWDRREAFHQSRLAESLTPYDSPLAQALAQLHLA
                     GFGDGSALAAVGRQLTGQSVLLASLDIFHFSGWLALALVPLCWLVRRPQAGAVVAAAD
                     "
     gene            complement(2949..4121)
                     /locus_tag="VHE37_08325"
     CDS             complement(2949..4121)
                     /locus_tag="VHE37_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVT35575.1"
                     /translation="MTIEQARNIRQRGFTLLALAVAAGVAAYASWWALEGRHYQDTDD
                     AYVSGDVVQVTSEVAGTVISLAADDTQGVQRGQNLLALDPADAQVAADRADADLARAV
                     RNVRALQAQSQQLRAQVNERTTALSRAQADLQRRASLVDSGAVSHEELAHARETVRQA
                     QADLAAAREQANVIDAQIENTTLQTHPQVQAAEAAVREAALALRRTHIAAPVDGVVAR
                     RAVQLGQRVAAGTPLLAVVPLDGVWVDANFKEVQLADLRIGQPVTLHADFYGDDVVYH
                     GRVAGLAAGSGSAFALLPAQNASGNWIKIVQRVPVRIALDADELKAHPLRIGLSMNAK
                     VDVRDTTGALIAASVRGTPLPQQASLAADPAVESRIARIVADNSSAATLAMAGAQP"
BASE COUNT          692 a         1481 c         1519 g          591 t
ORIGIN      
        1 agcgcgctca cccgcgggac tgaactgcat cacgcgtgag tcgcgcgcgc ggcgcgccca
       61 gcccagctgc tggatgcgcg cgagcagcgc accgccgagc acacccgcca ggtgctggcg
      121 ccgctcgctc cagtccaggc aggcgcggca cgccgcacgc ttgcctgaag cggcgcggtc
      181 cacgtcgatg ccgaaacgcg tgaaccactc gcgcccgcgc ggcgtcagcg ccaatgcatc
      241 gccgtcgtgg cgcagcgcct tgtgcgccag caagcgttcg taggccagca cgccgagctc
      301 gccggcgaga tggtcgtagc acacgcgcgc cttgcgcagc gccggttcgc gcgggccggg
      361 ccgcgcgcgc agtgcgccgg tgcggaaggc cacgcccatc agcgattcga gcagctgcgc
      421 cacgtcgcgg tcggccagcc tgaagtagcg gtgccggccc tggtgctcga ccgcgaccag
      481 gccagcgtcg cgcagcttgg acagatgtgc gctgatggtc tgcttggtca cgccggcgac
      541 atgcgccagt tcgctcgcgg taagcgcgcg gtcggccatc agcgcggtca gcacctcggc
      601 gcgggcctgg tcgccgatca gcgcggcgat gcgcacgatg ctggggccgt ctctcatggt
      661 tcgatgttaa acgaactata gacccggcgc ggcggcctat gctgtgcaca cgcaacctcg
      721 agcccgccgc atgaacatca cctgctttat ccggtaccag atcgacccgt tccagcgcga
      781 cgccttccgc cagtacgcgc ataactgggg ccgcatcatc ccgcgctgcg gcgggcagct
      841 ggtgggctac ttcctgccgc acgagggcag caacgacatc gcctggggcg tgatcgcgtt
      901 cgacagcctg gcggcgtacg agcgctaccg tgcgcggctg cagagcgacg accagggcaa
      961 ggccaacttc gcctttgcgc aggagcgccg gctgatcctg cgcgaggagc gcagcttcct
     1021 ggccggtgtc gacggcacgc tgaatgttgc ttgcaccatt gccgcgggcg ccgcgtgatc
     1081 gcggtgatct tcgaagtgct gccgcaggcg gcgcagcggc aggcctatct cgacacggcg
     1141 gccagcctgc gcccgctgct ggagcggatc gacggtttca tctccatcga gcgcttcgaa
     1201 agcctggcgc agcccggcaa gctgctgtcg ctgtcgtact ggcgcgacca ggcggcggtg
     1261 catcaatgga gaaccctgga agcgcatcgc gccgcgcagg ctgccgggcg tgcagaaatc
     1321 tttgccgact atcgcctgcg cattgcggcc gtggtgcgcg actacggcat gaacgaccgc
     1381 gcgcaggcgc cggaggattc gcggcgcgcg catcatgcct gatgctcagt ccgctgccgc
     1441 caccactgcg cccgcctgcg ggcggcgcac cagccagcac agcggcacca gcgccagcgc
     1501 cagccagccg gagaaatgga agatgtcgag gctggccagc agcacgctct ggccggtgag
     1561 ctggcgaccg accgcggcga gtgcggagcc atcgccaaat cccgcgaggt gcagttgcgc
     1621 gagcgcctgc gccagcggcg agtcatacgg cgtgagcgat tccgccaggc gcgactggtg
     1681 gaacgcttcg cggcgatccc agaacgtggt ggccagcgag gtggcgaagc tgctgccggt
     1741 gatgcgcagg aaattcgaca ggcccgaagc ggccggcact ttttccggcg gcaggccgtc
     1801 gagcaggatg gtcagcagcg cgacgaagaa catgcccatg gccaggccct gcaccagcag
     1861 cggcatgacc agcacggcga agctggcgtc ggcggtgtag ccggcgcgca tgaagtacga
     1921 cgccgagaac agcacgaatg acatcgtggc gaacacgcgc ggatcgatgc ggccgacgta
     1981 cctgccgacc agcggcgaca ccagcaccgc agccacgcca ctgggcgcgg ccaccaggcc
     2041 ggcccaggtg gcggtgtagc ccagctgcgt ctgcagccac agcggcagca gcaccacgtt
     2101 gccgaagaac accgcgtagc ccaggcacag cgccagcgtg ccgagggtga agctgcgcga
     2161 cttgaacaat gaaaggtcca cgatgggatg gcgctcggtc atctcccaga tgatccacgc
     2221 cgccagcgtg acggcagcga cgatcgccag caccacgatg gccggcgagt tgaaccagtc
     2281 gaggtccttg ccctggtcga gcagcacctg cagcgcgccg acccacagca ccagcaggcc
     2341 caggccgatg cggtcgatcg gcagcacgcg cgtgggcgtt tcgctggcgg cgagcgcgcg
     2401 ccagctgatg gcggcgcaga acaagccgac cggcacgttg atcaggaaga tccaggccca
     2461 tgaatagttg tcggagatga agccgccgag gatcgggccg cagatcggcg ccaccagcgt
     2521 ggtcatcgac cacaccgcca gcgcggcgcc tttcttgtcg gcggggaaca gcgagatgag
     2581 cagcgcctgc gagccgggga tcatcggccc ggaaaccgcg ccctgcagca cgcggaaagc
     2641 gatcagcgag gagatgttcc aggcgatgcc gcacagcaac gaggccgcgg tgaacgccag
     2701 caccgacagc acgaacacgc gcaccacgcc gtagcgctgc atcagccagc cggtcagcgg
     2761 taccgagatg ccgttggcca ctgcgaacga ggtgatgatc caggtgccct ggttggccga
     2821 gacgccgagg tcgccggcga tggtgggaat cgacacgttc gcaatcgtgg tgtcgagcac
     2881 ctgcatgaag gtgccgagcg ccagcgcgag gatggtcacc gcgcgcagca cgccggtgat
     2941 cggcgcgttc acggctgcgc cccggccatc gccagcgtcg cggcggaaga gttgtcggcg
     3001 acgatgcgcg cgatgcgcga ttccactgcg ggatcagccg ccaggctggc ctgctgcggc
     3061 agcggcgtgc cgcgcacgct ggcggcgatc aatgcgccgg tcgtgtcgcg cacgtccacc
     3121 ttcgcattca tcgacagccc gatgcgcagc ggatgcgcct tcagctcatc ggcgtcgagc
     3181 gcgatgcgca ccggcacgcg ctgcacgatc ttgatccagt tgccggacgc gttctgcgcc
     3241 ggcagcaggg cgaaggcgct gccgctgccg gcggcgaggc cggccacgcg gccgtggtac
     3301 accacatcgt caccataaaa gtccgcgtgc agcgtcaccg gctggccgat gcgcaggtca
     3361 gccagctgca cttccttgaa gttggcgtcg acccacacgc cgtcgagcgg caccacggcg
     3421 agcagcggcg tgccggcggc cacgcgctgg cccagctgca ccgcgcggcg ggcgaccacg
     3481 ccatccaccg gcgcggcaat gtgcgtgcgc ctgagcgcca gtgcggcctc gcgcaccgcc
     3541 gcttcggcgg cctgcacctg gggatgcgtc tgcaacgtgg tgttctcgat ctgcgcgtcg
     3601 atcacgttgg cctgctcgcg cgcggcggcg agatcggcct gcgcctggcg cacggtttcc
     3661 cgcgcgtgcg cgagttcctc gtgcgacacc gcgccgctgt cgaccaggct ggcgcgacgc
     3721 tgcaggtcgg cctgcgcgcg cgacaacgcg gtggtgcgtt cattcacctg cgcacgcagc
     3781 tgctgcgact gcgcctgcag cgcgcgcacg ttgcgcaccg cgcgggcgag gtcggcatcc
     3841 gcgcggtcgg cggccacctg cgcatcggcc gggtcgagcg cgagcaggtt ctgcccgcgc
     3901 tgcacgccct gggtgtcgtc ggcggccagc gagatcacgg tgccggccac ttcgctggtc
     3961 acctgcacca cgtcgccgct cacgtaggcg tcgtcggtgt cctggtagtg gcggccttcc
     4021 aatgcccacc acgaagcgta cgcggcgacg ccagcggcga ccgcgagggc cagcagggtg
     4081 aaaccgcgct ggcggatgtt cctggcttgt tcgatggtca tgggagtctc cgtttagttg
     4141 tgggaaggag tgaagtcgcc gcccgcggcg agcagcagcg ccacgcgcgc gctggcctgg
     4201 gcggcggcga gatcggcgcg cgtgcggcgc gcatcgagca actgggtttc ggcattgagt
     4261 acggtgaggt agcccgagat gcc
//
LOCUS       DASZZC010000389         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_860983,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000389 DASZZC010000000
VERSION     DASZZC010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860983"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VHE37_08330"
     CDS             <1..538
                     /locus_tag="VHE37_08330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVT35576.1"
                     /translation="ATQAREYLLINPRASKPTRNWPVSRNAEIARLAMARHGIGVALC
                     GGPGEDDRAAAREIAQAAPGVLDLSGQTDLPKLFALIAGARVLLSPDSGPVHIARAFD
                     VPVVGLYADARSAKTGPYRRMRWSVDHWADAVKSILGKDPSRVPWDTRIHDARSMELI
                     TVSEVADKLAAALAAARG"
     gene            548..1354
                     /locus_tag="VHE37_08335"
     CDS             548..1354
                     /locus_tag="VHE37_08335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT35577.1"
                     /translation="MDKISAYIIAYNEAEKIGDAIRSVLWADEVVVVDSNSTDGTVQI
                     AEQLGARVVQVAFKGFGDLRNQAVAACRHPWIFSLDSDERATPEVRDEVLQTIARADA
                     LDAYLVPRRNFFMGRWIRHSGWYPNYRQPQLYRRDALVYGDDAVHETWRLTTGKPVGT
                     LTQPVWQVPFRNLEESLRKTNRYSTLSIYRLQHKRPGLLTALAHAKWAFIRHYFLKLG
                     ILDGAAGFVIAFGYFEQTFYRYMKRHEQAAGWELPRQKEPIVRNAPVGEH"
     gene            1412..2455
                     /locus_tag="VHE37_08340"
     CDS             1412..2455
                     /locus_tag="VHE37_08340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVT35578.1"
                     /translation="MRLHAGSRIGIVQAFNIGDVVACLPLAGLIKRHYPGSRIVFVGR
                     CYAEAVALACVHIDEFLDAQACLHDPQVLARAKLDVLINPFPHREIAQAAYQARVPVR
                     VGNLRRGAYARWCNRFVFYGRRGTGLHEAQLNLRDLRAMGIRDNVDLPGLAALMGLTR
                     LAPLEAQHRALLDPARFNLLLHPRSNGNSREWPLAHYLALIQQLPPRFKVFLTGTEAD
                     AQTIEQNFPQLLRAPGVTPLFGRFTLPQFISFIAAADGMVAASTGPLHLAAALGRHAL
                     GIYPSRAGLNAKRWGPVGPRAQVAQLAPACPYAGKSCKRVGDGLACACTEAVTPASVL
                     ATLAQWQALQRPS"
     gene            complement(2440..>3240)
                     /locus_tag="VHE37_08345"
     CDS             complement(2440..>3240)
                     /locus_tag="VHE37_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35579.1"
                     /translation="KLGRFIGRRMRRRRHVVDVNLRLCFPQLTADERARLTSAHFAAL
                     GRGLFESGLAWFASDRRLAPHGRVEGLEHFHGATRNGRGALLLTGHFTTLEIGARYLC
                     IAGVPFHAMYRPINNAVIDFCMHRWRQRRSGLPALPKDDLKKLVRALRGGASIWYGPD
                     QTLELRGALKVPFFGVPVLTVTATSRLAELGRAAVVPYMPRREGAKYIVQFQPALEPF
                     PTGDDLADTTRVNHAIEAGVLAAREQYFWVHRRFKPFRPGDVDVYDGR"
BASE COUNT          515 a         1135 c         1109 g          481 t
ORIGIN      
        1 ggcgacccag gcgcgcgaat acctgctgat caacccgcgc gcgagcaagc ccacacgcaa
       61 ctggcccgtc tcgcgcaatg cggagatcgc ccggctggcg atggcgcgcc acggcatcgg
      121 cgtggcgctg tgcggcggac ccggcgagga cgatcgtgcc gccgcgcggg agatcgcgca
      181 ggccgcgccc ggcgtgctcg acctgtcggg ccagaccgac ctgcccaagc tctttgcgct
      241 gatcgccggc gcgcgcgtgc tgctgtcgcc ggattccggg ccggtacaca tcgcgcgcgc
      301 gtttgatgtg ccggtggtcg ggctgtacgc cgacgcgcgc tcggccaaga ccgggccgta
      361 ccggcgcatg cgctggagcg tcgatcactg ggccgacgcg gtgaagtcga tcctcggcaa
      421 ggacccgtcg cgggtgccgt gggacacgcg catccatgac gcgcgctcga tggaactgat
      481 cacggtgagc gaggtggcgg acaagctggc cgccgcactt gcagcggcgc gcggctagac
      541 tcggcgcatg gacaagattt ccgcctacat catcgcctac aacgaggccg aaaaaatcgg
      601 cgatgccatc cgcagcgtgc tgtgggccga cgaggtggtg gtggtggatt ccaacagcac
      661 cgacggcacc gtgcagatcg cggaacagct gggcgcgcgc gtggtgcagg tggcattcaa
      721 gggtttcggc gacctgcgca accaggcggt ggcggcctgc cgccacccgt ggatcttcag
      781 cctggactcc gacgagcgcg ccacgccgga ggtgcgggac gaagtgctgc agaccatcgc
      841 gcgcgccgat gcgctcgacg cctacctcgt gccgcggcgc aatttcttca tgggccgctg
      901 gatccgccac tccggctggt acccgaacta ccgccagccg cagctgtacc ggcgcgatgc
      961 gctggtctat ggcgacgacg cggtacacga aacctggcgc ctcaccaccg gcaagccggt
     1021 cggcacgctg acccagccgg tgtggcaggt gccgttccgc aacctggaag agtcgctgcg
     1081 caagaccaac cgctactcga cgctgtcgat ctaccggctg cagcacaagc gaccggggct
     1141 gcttacggcg ctggcgcatg ccaagtgggc gttcatccgg cactacttcc tcaagctcgg
     1201 catcctcgac ggcgcggccg gtttcgtcat cgccttcggc tacttcgagc agaccttcta
     1261 ccgctacatg aagcgccacg agcaggccgc cggctgggaa ctgccaaggc aaaaagaacc
     1321 tattgtgcgc aacgccccgg tcggcgagca ttgattcaaa gaatccgcgg cctcctggac
     1381 cgcggcctgg ggtcaggtcg gggaaacgtg aatgcgccta catgcgggca gccgcatcgg
     1441 catcgtgcaa gccttcaaca tcggcgacgt ggtcgcgtgc ctgccgctgg ccggcctgat
     1501 caagcgccac tacccgggtt cacgcatcgt gttcgtcggc cgctgctacg ccgaggcggt
     1561 ggcgctggcc tgcgtacaca tcgacgaatt tctcgacgcg caagcctgcc tgcatgatcc
     1621 gcaggtgctg gcgcgggcga aactcgacgt gctgatcaac ccgtttccgc accgcgagat
     1681 cgcgcaggcc gcgtatcagg cgcgcgtgcc ggtgcgcgtg ggcaatttgc gccgcggcgc
     1741 gtatgcacgc tggtgcaacc gcttcgtgtt ctacggccgc cgcggcacgg gactgcacga
     1801 ggcgcagctg aacctgcgcg acctgcgcgc gatgggcata cgcgacaacg tcgacctgcc
     1861 cggactcgcc gcgctgatgg gactcacccg tctcgcgccg ctggaagcgc agcaccgcgc
     1921 gctgcttgat ccggcgcgct tcaacctgct gctgcatccg cgctccaacg gcaacagccg
     1981 cgagtggccg ttggcgcatt acctcgcgct gatccagcag ctgccgccgc gcttcaaggt
     2041 gttcctcacc ggcaccgagg ccgacgcgca aaccattgag cagaattttc cgcagctgct
     2101 gcgtgcgcca ggcgtgacgc cgctgttcgg acggttcacg ctgccgcaat tcatttcatt
     2161 catcgctgcc gccgacggca tggtggccgc gagcaccggc ccgctgcacc tggccgcggc
     2221 gctcggccgc catgcgctgg gcatctaccc gagtcgcgca gggctcaatg caaaacgctg
     2281 gggcccggtc ggcccgcgcg cgcaggtcgc gcagctggcg ccggcctgcc cgtacgcggg
     2341 caagagctgc aagcgcgtgg gcgatgggct ggcctgcgcc tgcaccgagg cggtgacgcc
     2401 agcgtcggtg ctggccacgc tcgcacagtg gcaagcactt cagcggccgt cgtaaacgtc
     2461 cacgtcgccg ggccggaacg gcttgaagcg ccggtgcacc cagaaatact gctcgcgcgc
     2521 ggccagcacg cccgcttcga tcgcatgatt cacgcgcgtg gtgtcggcca gatcgtcgcc
     2581 ggtcggaaac ggctcgagcg ccggctggaa ctgcacgatg tatttcgcac cttcgcgccg
     2641 cggcatgtac ggcaccactg ccgcgcgccc gagttccgcc aggcgcgaag tcgcggtcac
     2701 cgtgagcacc ggcacgccga agaacggcac cttgagcgcg ccgcgcagct cgagcgtctg
     2761 gtccggcccg taccagatgc tggcgccgcc gcgcagcgcg cgcaccagct ttttcagatc
     2821 gtccttgggc aatgccggca ggcccgagcg ccgctggcgc cagcggtgca tgcagaaatc
     2881 gatcaccgcg ttgttgatcg gccggtacat ggcgtggaac ggcacgccgg cgatgcacag
     2941 gtagcgcgcg ccgatctcca gcgtggtgaa gtgcccggtg agcagcagcg cgccgcggcc
     3001 gttgcgcgtc gccccgtgga aatgctcgag accctcgacg cgcccatgcg gcgccagccg
     3061 gcggtcggag gcgaaccagg ccaggcccga ctcgaacagc ccgcgcccca gcgcggcgaa
     3121 atgcgcgctg gtcaggcgcg cgcgctcgtc ggcggtgagt tgcggaaaac acaggcgcag
     3181 gttgacgtcg accacgtggc ggcggcggcg catgcgccgg ccgatgaagc gccccagctt
//
LOCUS       DASZZC010000390         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_997498,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000390 DASZZC010000000
VERSION     DASZZC010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997498"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VHE37_08350"
     CDS             complement(<1..275)
                     /locus_tag="VHE37_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HVT35580.1"
                     /translation="MKQNSVQPVILAALAASVVIAAAKFVAAVLSGSSSMLAEGMHSS
                     IDIVNQALLLYGLKRARRPPDREFPFGYGKEIYFWSFVVAIQIIMVGA"
     gene            complement(431..2293)
                     /locus_tag="VHE37_08355"
     CDS             complement(431..2293)
                     /locus_tag="VHE37_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVT35581.1"
                     /translation="MPSRIEDYALIGDGHTAALVGRDGSIDWLCWPRFDSAACFAALL
                     GSPRHGRWLLAPAAEPRSVSRHYGDGTLILHTEFETPEGAVSVIDCMPAGVGASQILR
                     QVRGRRGRVPMRMELVLRFDYGSTVPWVSRLPYAGGLRAVAGPHMCVLRSDVETHGEN
                     RTTVASFEIEAGQCVNFSLAYGPSHLSPPSAADPRQALDAATRDGRDWSARCTYRGPY
                     EDAVRESLRVLRGLIYAPTGGIVAAPTTSLPEQLGGVRNWDYRYCWLRDATLTLLALM
                     NCGYYDEAASWRSWLERTAAGSPEQIQIMYGIAGERRLQEWLVEWLPGYQGSAPVRVG
                     NAAAGQLQLDVYGEVMDALHQGRRGGLATDEAGWELQRALLQHLGRAWTQPDEGIWET
                     RGGKRHFTFSKVMAWVAYDRGIRSAERYRLEAPLEEWKRTRAAIHADVCAHALDAKRG
                     CFVQSYGSCTLDAALLQIPLVGFLPPDDVRVRATLDAIERDLISDGLVMRYDSRRVKD
                     GLPAGEGAFLACSFWLVDNLVLAGRHDDARKLFERLLALRNDVGLLAEEYDPRARRLV
                     GNFPQAFSHIALINSALQLSRAQGPTQQRSHDAQDLRGASNTPEQAQKPFTAPR"
BASE COUNT          382 a          841 c          831 g          366 t
ORIGIN      
        1 gcgccgacca tgatgatctg gatggcgacg acgaagctcc agaaatagat ttccttgccg
       61 tagccgaacg ggaattcgcg gtccgggggc cgccgcgcgc gcttgaggcc atagagcagc
      121 agggcctggt tgacgatgtc gatcgaggaa tgcatgcctt cggccagcat cgaggagctg
      181 cccgacagca cggccgccac gaacttggcc gcggcgatga ccacgctggc ggcaagcgcg
      241 gccaggatca cgggctggac ggagttctgc ttcatgcgac cgggatgcgc gtgggcggat
      301 gcatggaatt gggcgcggcg tagagcggcg aagttcgcac ggggccgccg aatcatgtcc
      361 accgcacgcc gtttttcgat gttattaaat attgattctt ttaactttaa acaatattta
      421 ataacatgag tcagcggggt gcggtgaagg gtttttgcgc ctgttccggc gtgttcgagg
      481 ccccgcgcag atcctgcgcg tcgtgcgagc gctgctgcgt gggcccctgc gcgcgcgaca
      541 gctgcagcgc gctgttgatc agcgcgatgt gcgagaaagc ctgcggaaaa ttgccgacca
      601 gccggcgcgc gcgcggatcg tattcctcgg ccagcaggcc cacgtcgttg cgcagcgcca
      661 gcagccgctc gaacagcttg cgcgcgtcgt cgtggcgccc ggccagcacc aggttgtcga
      721 ccagccagaa gctgcaggcg aggaacgcgc cctcgcccgc cggcagcccg tccttcaccc
      781 gccggctgtc gtagcgcatc accaacccgt cgctgatcag gtcgcgctcg atggcgtcga
      841 gcgtcgcgcg cacgcgcaca tcgtccggcg gcaggaagcc caccagcggg atctgcagca
      901 gcgcggcgtc gagcgtgcaa ctgccgtagc tctgcacgaa gcagccgcgc ttcgcgtcga
      961 gcgcgtgcgc gcacacgtcg gcatggatcg ccgcgcgcgt gcgtttccac tcctccagcg
     1021 gcgcctcgag ccggtagcgc tccgcggaac ggatgccgcg gtcgtaggcc acccacgcca
     1081 tgaccttgga aaaagtgaaa tgccgcttgc cgccgcgcgt ttcccagatg ccttcgtccg
     1141 gctgggtcca ggcgcgcccc aggtgctgca gcagcgcgcg ttgcagttcc cagccggcct
     1201 cgtcggtggc caggccgccg cggcggccct ggtgcagcgc atccatcact tcgccgtaca
     1261 cgtccagctg cagctgtccg gccgcggcat tgcccacgcg caccggcgcc gaaccctggt
     1321 agcccggcag ccattccacc agccattcct gcaggcgccg ctcgccggcg atgccgtaca
     1381 tgatctggat ctgctcgggc gaaccggccg cggtgcgctc cagccagctg cgccacgagg
     1441 ccgcctcgtc gtagtagccg cagttcatca gcgccagcag ggtgagcgtg gcgtcgcgca
     1501 gccagcagta gcggtaatcc cagttgcgca cgccgcccag ctgctcgggc agcgaggtgg
     1561 tcggcgccgc cacgatgcca cccgtgggcg catagatcag tccgcgcagc acgcgcagcg
     1621 actcgcgcac cgcgtcttca tacgggccgc ggtaggtaca gcgcgcgctc cagtcgcggc
     1681 cgtcgcgggt ggccgcatcc agcgcctggc gcggatcggc cgccgacggc ggcgacaggt
     1741 gcgacggccc ataggccagc gagaaattca cgcactgccc cgcctcgatc tcgaagctgg
     1801 ccaccgtggt cctgttctcg ccgtgcgtct ccacgtcgct gcgcagcacg cacatgtgcg
     1861 ggccggccac tgcgcgcagc cctcctgcgt acggcaggcg cgagacccat ggcacggtgg
     1921 acccgtagtc gaagcgcagc accagttcca tgcgcatcgg cacgcggccg cggcgcccgc
     1981 gcacctggcg caggatctgc gaggcgccga cgcccgccgg catgcagtcg atgacgctga
     2041 cggcgccttc cggcgtctcg aactcggtgt gcaggatcag cgtgccgtcg ccgtaatggc
     2101 gcgacaccga acgcggctcc gccgccggcg ccagcagcca gcggccgtga cgcggactgc
     2161 cgagcagcgc ggcgaagcag gcggcggaat cgaagcgcgg ccagcacagc cagtcgatgg
     2221 aaccgtcgcg cccgaccagc gcggcggtgt ggccgtcgcc gatcagcgcg tagtcttcga
     2281 tgcgggaggg catgaaaatt cgagcggccg cggcgcggcc gggttcgcgg aaattttttc
     2341 ccggggagaa atcagtccgc gtgatgcgtg gcgagatact gccgcgtgcg gtcctcgcaa
     2401 cgctggcgtg ccagctgcgc
//
LOCUS       DASZZC010000391         8252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_861130,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000391 DASZZC010000000
VERSION     DASZZC010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861130"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1137
                     /locus_tag="VHE37_08360"
     CDS             130..1137
                     /locus_tag="VHE37_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVT35582.1"
                     /translation="MNSNRLPPLNALRAFEAVARNRSFRKAADELFVTPAAVTHQIKQ
                     LEEQLGIALFERKSRGIALTPAASAALPRFRQAFELMSQGVAELRGHGNVPRLSVRAT
                     PTFMSRWLMPRMQSFLALHPNVELHLLASDRMISPSPWQTQPEQEVAQAMTPDIDIQF
                     SSGPPAGDIVDLLLMVDVVPMCNPALLKGAHPLREPDDLRHHTLLHADGLLSDRMRST
                     WALWLQHAGARLVDPRRGMQFDHSTLALEAAADGLGVTLAMPLLAAGELERGEVKIAF
                     AQALPLGRGYYSVISSVADGRPEVAAFRQWLQREAADTASALAAKKEGRVRRGLSARS
                     R"
     gene            1353..1508
                     /locus_tag="VHE37_08365"
     CDS             1353..1508
                     /locus_tag="VHE37_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35583.1"
                     /translation="MDSLTTLVEVVVLAATTGIFFSMMVMDWLDTRGADAQRPATRRR
                     GSALRLG"
     gene            complement(1505..1717)
                     /locus_tag="VHE37_08370"
     CDS             complement(1505..1717)
                     /locus_tag="VHE37_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35584.1"
                     /translation="MDQSKADGTAAAGRNPEDIEHLLDGMVNWTTAAGVLLALALVCA
                     QVAAHLHPPPGAAAMHSRGNAVEPRV"
     gene            complement(1836..2129)
                     /locus_tag="VHE37_08375"
     CDS             complement(1836..2129)
                     /locus_tag="VHE37_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35585.1"
                     /translation="MNKIIAVAVLALSSAPSFAAGPLQPVAAVINAPAARIGGLAPAT
                     VALPPVTIATPGIGKGWSKSVAVPLPGLGALDVSDTNQGRGGSHTLKLQVVPD"
     gene            complement(2141..3673)
                     /gene="glgA"
                     /locus_tag="VHE37_08380"
     CDS             complement(2141..3673)
                     /gene="glgA"
                     /locus_tag="VHE37_08380"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HVT35586.1"
                     /translation="MASLRVLHVAAECYPLAKTGGLGDVVAALPAAQRAIGIDACVAL
                     PGYRGLRDCLREPKALTRFAAGGEQFTVIAGRVHDEQNSLPVLLFECPPLYDRGGDPY
                     RDAHGVEFTDNARRFARFCEAVAIYACGHAPFDLLHLHDWQAALAAGWLGQQGQRPRV
                     LYSIHNLAYQGHVAADRVQQLQLPPPTLAAGTETWGGFSCMKAGIRLADAVATVSPGY
                     AREILTPAYGYGMEAALRECAGGVHGILNGIDTQLWNPRSDHQLERNYSEHTLAEGKR
                     ANKLALQRELGLAESDAPLLAYIGRLAEQKGADLILQAREALLALPLQLVVLATGDLP
                     LQQAFREFADAAPAGQVAVRLLHDERLAHRINAAADLLLMPSRYEPCGLNQMYAQRYG
                     TLPLVRRTGGLADSVVDATDASIADGSATGVQFLDADAGGVLYAVRRGLQLHADEALR
                     TRLQRAGMARDFSWSLAAQQYQQLYRQLCPAPVAPADSTAATGIFPTGNDVAAAQHPL
                     PA"
     gene            complement(3691..4122)
                     /locus_tag="VHE37_08385"
     CDS             complement(3691..4122)
                     /locus_tag="VHE37_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35587.1"
                     /translation="MHPNQELLVDDLWGDHGSVRALLRRFELSESPDDRRDAMRLATD
                     LFSVHSEIEERIASGYAQHRALQAIIHLMEQLETTDPRSKLYLARGMELKQRLEEYIA
                     VEEQTAATPSSGSLKAEPYNVDLLGLRTNLRDNATRLLRVH"
     gene            complement(4146..4370)
                     /locus_tag="VHE37_08390"
     CDS             complement(4146..4370)
                     /locus_tag="VHE37_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35588.1"
                     /translation="MHLEEALDMTIDKFARRNLVISERLNQIMQREQESTRDPEALLS
                     PRLRELFAAQERLLTAAERCLGHGGLSVAR"
     gene            4625..5878
                     /gene="glgC"
                     /locus_tag="VHE37_08395"
     CDS             4625..5878
                     /gene="glgC"
                     /locus_tag="VHE37_08395"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HVT35589.1"
                     /translation="MSDRDARFVSRLTHDTLALVMAGGRGTRLMQLTQGGAKPAVPFA
                     GKFRIIDFTLSNCINSGIRRVGVLTQYKSHSLIRHLQKGWSFLRGQFGEFIEMLPAEQ
                     RTESASWYAGTADAVFQNLDFVRRHQPAHVLILAGDHVYKMDYGRMLAHHARCGARIT
                     VGCLEVPLDEASSFGVMETDASSRIVAFQEKPQQPRSMPGKPGSALASMGIYVFEARF
                     LLDWLARDAGVLESSHDFGKDIIPRAIEQGDVYAYPFTDLYDSSRQGYWRDVGTVDTY
                     WKGNLELIEVVPELNLYDEDWPIWTYEEQLPPAKFVFDDEGQRGSAVDSLVSSGCIVS
                     GASVRRSVLFSRVHLERGSQVEESLLLPGTAVGANCRIVRAIIDENCAIPAGSEIGVD
                     LERDRRRFHVSEQGVTLVTRDMLEH"
     gene            5903..7861
                     /locus_tag="VHE37_08400"
     CDS             5903..7861
                     /locus_tag="VHE37_08400"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HVT35590.1"
                     /translation="MSASAATAMLPQDPALAAALAAPRLVIARVRPCVDGGRYPGKAI
                     VGQPLAIEADVFGDGHEQLAAELRWGNDAPLPMEELGNDRWRTWVTPARIGMFEFRID
                     AWVDPYASHVHDVRKKREAQQPIKRELAEGVALLQRAQQRSGAAMLGALLDALAAADE
                     VRQYELLTSAATLGTMRQLAERPFLQRSPAYAVDVERLRAGFGAWYELFPRSQSGDAG
                     RHGTFHDVVHRLPAIKAMGFDVLYLPPIHPIGHTHRKGRNNSLVAQPGDPGSPYAIGN
                     ELGGHDAIHPELGGIEGFRRLRQACAEHGLEIALDFAIQCSPDHPWIRQHPEWFSWRS
                     DGSLRHAENPPKKYEDIVNVDFYAPGAQPSLWLALRDVVLHWVGLGVKIFRVDNPHTK
                     PLPFWQWLIADVRAAHPEVIFLAEAFTRPAMMYRLGEIGFSQSYTYFTWRHSRAEFIE
                     YLTELTQGPARDSYRPNFFVNTPDINPYFLHNSGRAGFVIRAALAATLAGSWGLYSGF
                     ELCESAPLPGKEEYLDSEKYQLRPRDWSAPGNIIAEISALNRIRREHPALQSHLHMRF
                     LPSSNEQVLFFEKTAPGTADRLLIAITLDPWTPQDAWLEIPDGGPYEVQELMRGYRFT
                     WRGRTQHWRFIPQEMPVAIWQLEASHAA"
     gene            7851..>8252
                     /locus_tag="VHE37_08405"
     CDS             7851..>8252
                     /locus_tag="VHE37_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVT35591.1"
                     /translation="MPPDSGTQSLKQFEARARQDTHWYKDAVIYQLHVKSFYDANNDG
                     VGDFAGLIEKLDYIQSLGVTCIWLLPFYPSPRRDDGYDIAEYRGIHPQYGSLADARRF
                     IKEAHKRKLRVITELVVNHTSDQHPWFQRARR"
BASE COUNT         1360 a         2828 c         2741 g         1323 t
ORIGIN      
        1 ccatgctcgc agcatgggcc aaggcagcgc gcgctgcatt gatccagatc aagcgggttt
       61 ttgcggctga catggatttg ccgcgggtgc cccaacataa tgtcgcgcga tgtgcccgtg
      121 tctcctttca tgaactccaa ccgtctgccg ccgctcaacg cgctgcgcgc cttcgaggcg
      181 gtggcgcgca accgcagctt ccgcaaggcc gcggacgaac tgttcgtcac gccggcggcg
      241 gtcacccacc agatcaagca gctggaagag cagctgggca ttgcgctgtt cgagcgcaag
      301 agccgcggca tcgcgctgac gccggcggcc agcgccgcgc tgccgcgctt ccgccaggcc
      361 ttcgagctga tgtcgcaggg cgtggccgag ctgcgcggcc acggcaacgt gccgcgcctg
      421 tcggtgcgcg ccacgccgac cttcatgtcg cgctggctga tgccgcgcat gcagagcttc
      481 ctggcgctgc atcccaacgt ggagctgcac ctgctggcca gcgaccgcat gatcagccct
      541 tcgccgtggc agacccagcc ggagcaggag gtcgcgcagg cgatgacgcc ggacatcgac
      601 atccagttca gcagcgggcc gccggctggc gacatcgtcg acctgctgct gatggtcgat
      661 gtggtgccga tgtgcaatcc ggcgctgctc aagggcgcac acccgctgcg cgagccggac
      721 gacctgcgcc atcacacgct gctgcacgcc gacggcctgc tgtcggaccg catgcgctcg
      781 acctgggcgc tgtggctgca gcacgcgggc gcgcggctgg tcgatccgcg ccgcggcatg
      841 cagttcgacc actcgacgct ggcgctggaa gcggcagccg acggcctggg cgtgacgctg
      901 gccatgccgc tgctggccgc cggcgaactc gagcgcggcg aagtgaagat cgctttcgcg
      961 caggcgctgc cgctgggccg cggttactac tcggtgattt cgtcggtggc cgacggccgc
     1021 ccggaagtgg cggcgttccg ccaatggctg cagcgggagg cggccgatac cgccagtgcg
     1081 ctcgcggcaa aaaaagaagg ccgcgtgaga cgcggcctga gtgcgagatc ccgttgaagg
     1141 gatctggagg agatcggcga catcgaaatg tctggagcgc attaggcccc agcgccaccg
     1201 cagcagcaat tgatcttttt cttgcggggg gccaagaaaa actaaacgcg ctttcgtcgc
     1261 cgctttagat tttgtacgcg gtggccgcaa gcaaaatcat ttgttgcctc gatgcccggc
     1321 tccgatactg ccaccgcatc aggaggtgca acatggactc gctgacgacg ctggttgaag
     1381 tggtggtgct ggctgccacc accggcatct tcttctcgat gatggtgatg gactggctgg
     1441 acacgcgcgg ggccgacgcg cagcggccgg caacgcggcg tcgcggcagc gccctgcggc
     1501 tgggctagac gcgcggctcg accgcgttgc cgcgcgagtg catggcggcc gcgccgggcg
     1561 gagggtgcaa atgcgcggca acctgggcgc agaccagcgc cagcgccagc agcacgccgg
     1621 cagccgtggt ccagttgacc atgccgtcca gcaggtgctc gatatcttcg ggattgcggc
     1681 ccgcggccgc cgtgccatcc gccttgctct ggtccatcgc atcctccgcg gccgatctgc
     1741 gtcggccctc ctccatgaac tagagaggcc gggcggcgcg aagagttcga gatttttttg
     1801 cggaggcgcc gtgcgcagga cagcgccgcg ggaaatcagt cgggaaccac ctgcagcttg
     1861 agcgtgtgcg aaccgccgcg cccctggttg gtgtcggaaa catccagcgc gcccaggccc
     1921 ggcagcggca ccgccacgct cttgctccag cccttgccga tgccgggcgt ggcgatggtc
     1981 accggcggca gcgcgacggt ggccggggcc agcccgccga tcctggccgc gggcgcgttg
     2041 atcaccgccg ccaccggctg gagcgggccg gccgcgaacg aaggtgcgct ggacagcgcc
     2101 agcacggcga ccgcgatgat cttgttcatg ggcaattcct tcaggccggc aagggatgct
     2161 gcgctgctgc cacatcattt cctgtcggaa atatacccgt tgcagccgtg gaatctgcag
     2221 gagcaaccgg cgccgggcac aactgccggt acagctgctg gtattgctgc gcagccagcg
     2281 accatgagaa atcgcgcgcc atgccggcgc gctgcaggcg cgtgcgcagc gcttcgtcgg
     2341 catgcagctg cagtccgcgc cgcacggcat agagcacgcc gcccgcatcg gcgtcgagga
     2401 actggacgcc ggtggcgctg ccgtcggcga tgctggcatc ggtcgcatcg accaccgagt
     2461 ccgccaggcc gccggtgcgg cgcaccagcg gcagcgtgcc gtagcgctgc gcgtacatct
     2521 ggttgaggcc gcagggctcg tagcgcgacg gcatcagcag caggtcggcc gcggcgttga
     2581 tgcggtgggc cagccgttcg tcatgcagca ggcgcacggc cacctgcccg gccggcgcgg
     2641 catcggcgaa ttcgcggaac gcctgctgca gcggcaggtc gcccgtggcc agcaccacca
     2701 gttgcagcgg cagcgccagc agcgcctcgc gcgcctgcag gatcaggtcg gcacctttct
     2761 gctcggccag ccggccgata tacgcgagca gcggcgcatc gctttcggcc agccccagtt
     2821 cgcgctgcag ggcgagcttg ttggcgcgct tgccctcggc cagcgtgtgc tccgaatagt
     2881 tgcgctccag ctggtggtcg ctgcgcggat tccacagctg cgtgtcgatg ccgttgagga
     2941 tgccatggac cccgcccgcg cactcgcgca atgccgcctc catgccgtag ccgtatgcgg
     3001 gcgtcaggat ctcgcgcgca taacccgggc tcaccgtggc cactgcatcg gccaggcgga
     3061 tgccggcctt catgcaggaa aaccctcccc aggtttcagt gcccgccgcc agcgtgggcg
     3121 gcggcaactg cagttgctga acacggtccg ccgccacgtg accctggtag gccaggttgt
     3181 ggatgctgta cagcacgcgc ggccgctggc cctgctggcc cagccagccg gcagccagtg
     3241 cggcctgcca gtcatgcagg tgcagcaggt cgaacggggc atgcccgcag gcatagatgg
     3301 ccacggcttc gcagaagcgc gcaaagcgcc gcgcgttgtc ggtgaactcc acgccgtgcg
     3361 catcgcggta tggatcgccg ccgcggtcgt acagcggcgg gcactcgaac agcagcaccg
     3421 gcagcgagtt ctgctcatcg tgcacgcggc ctgcgataac ggtgaattgt tcgccgccgg
     3481 cagcaaagcg cgtcagcgcc ttgggctcgc gcaggcaatc gcgcaggccg cggtagcctg
     3541 gcagcgccac gcacgcatca atgccgatgg cgcgctgtgc ggcgggcagc gcggcgacca
     3601 cgtcgccaag gccaccggtc ttggccagcg gatagcactc cgccgccaca tgcaggaccc
     3661 gcaggctagc catggctccc cccgcgctgc tcaatggacg cgcagcagcc tcgtggcgtt
     3721 gtcgcgcagg ttggtgcgca ggccgagcag gtcgacgttg taaggctcgg ccttgagcga
     3781 gccgctcgac ggcgtggccg ccgtctgctc ctccaccgcg atgtactcct ccagccgctg
     3841 cttgagttcc atgccccgcg ccaggtacag cttgctgcgc gggtcggtgg tctccagctg
     3901 ctccatcaaa tggatgattg cctgcagcgc gcggtgctgc gcgtaacccg aagcgatgcg
     3961 ctcctcgatc tcggaatgga ccgagaacag gtcggtcgcc agccgcatgg catcgcgccg
     4021 gtcgtcgggc gattcggata actcaaagcg gcgcagcagc gcgcgcaccg atccgtggtc
     4081 gccccagagg tcgtcgacca gtaattcctg atttgggtgc atccttgccc cctcgatgat
     4141 gctgatcagc gggcgaccga caggccgcca tggcccaggc aacgctccgc tgcggtcaac
     4201 agacgctcct gcgcggcaaa cagttcacgc aatcgcggcg acagcaacgc ttccgggtcg
     4261 cgcgtgcttt cctgctcgcg ctgcatgatc tgattcaggc gctcggaaat caccaggttc
     4321 ctgcgcgcga acttgtcgat cgtcatatcc agggcctctt ccagatgcat gttgtgtttc
     4381 ctcactcctg cgcctgctca cgcggcaggc atcccgctgc aaggcagcgg ccatgccaac
     4441 tgcccggcta agcacgcaat gcctagacaa acaaaaactt ggaaatgcac agctcgtgtc
     4501 tgccgtctcg tcggctgaaa atttttcctg cgctgcgctg aaaaaaatct acgggcgcgc
     4561 ggccggctga acaatttgca agcgcacccg ctctaacccc gtccacaaaa tgtggcgggg
     4621 gaccatgagc gatagagatg cccgtttcgt cagccggctg acccatgaca cgctggcgct
     4681 ggtgatggcc ggcggccgcg gcacgcgcct gatgcagttg acgcagggcg gggccaagcc
     4741 ggcagtaccg ttcgccggca agttccgcat catcgacttc accctgtcca actgcatcaa
     4801 cagcggcatc cgccgtgtgg gggtgctcac gcagtacaag agccactcgc tgatccggca
     4861 cctgcagaag ggctggagct tcctgcgcgg gcagttcggc gaattcatcg agatgctgcc
     4921 ggccgagcag cgcaccgaga gcgcctcgtg gtacgccggc acggccgatg ccgtgttcca
     4981 gaacctggat ttcgtgcggc gccaccagcc ggcacacgtg ctgatcctgg ccggcgacca
     5041 tgtctacaag atggactacg ggcgcatgct ggcgcaccac gcgcgctgcg gcgcgcgcat
     5101 caccgtcggc tgcctggaag tgccgctgga cgaagccagc agcttcggcg tgatggaaac
     5161 ggatgcctcc agccgcatcg tcgccttcca ggaaaaaccg cagcagccgc gctccatgcc
     5221 gggcaagccg ggcagcgcgc tggccagcat gggcatctac gtgttcgagg cgcgcttcct
     5281 gctcgattgg ctggcgcgcg acgccggcgt gctcgagtcg agccacgact tcggcaagga
     5341 catcattccg cgcgccatcg agcagggcga cgtctatgcc tacccgttca ccgacctgta
     5401 cgactccagc cgccagggct actggcgcga tgtcggcacc gtcgacacct actggaaagg
     5461 gaatctcgaa ctgatcgagg tggtgccgga actcaacctg tacgacgagg actggccgat
     5521 ctggacctac gaggaacagc tgccgccggc caagttcgtg ttcgacgacg agggccagcg
     5581 cggcagcgcg gtggattcgc tggtgtcttc cggctgcatc gtctccggtg cctcggtgcg
     5641 ccgcagcgtg ctgttttcgc gcgtgcacct ggagcgtggc agccaggtgg aggaatcgct
     5701 gcttctgccc ggcacggcag tgggggcgaa ctgccgcatc gtgcgcgcga tcatagatga
     5761 gaactgcgcg attcccgccg gcagtgagat cggggtcgat ctcgaacggg atcgccgccg
     5821 cttccatgtc agcgagcagg gcgtgacgct ggtgacgcgc gacatgctcg agcactgatc
     5881 caccgcaggt ccaaggaggg ccatgtccgc aagcgccgcc accgccatgc tgccgcagga
     5941 ccctgcgctc gccgccgcgc tggcggcgcc gcgcctggtc atcgcgcgcg tgcgcccatg
     6001 cgtggatggc ggccgttatc ccggcaaggc catcgtcggc cagccgctcg ccatcgaggc
     6061 tgatgttttc ggcgacggcc acgagcagct cgccgccgaa ctgcgctggg gcaatgacgc
     6121 gccgctgccg atggaggaac tgggcaacga ccgctggcgc acctgggtga cgccggcgcg
     6181 catcggcatg ttcgagttcc gcatcgacgc ctgggtggat ccttacgcca gccacgtgca
     6241 tgacgtgcgc aagaagcgcg aagcgcagca gccgatcaaa cgtgaactgg ccgaaggtgt
     6301 cgcgctgctg cagcgcgcgc agcagcgcag cggcgccgcc atgctcggcg cgctgctcga
     6361 cgcgctggct gccgcggacg aagtccggca atacgaactg ctgaccagcg ccgccacgct
     6421 cggcaccatg cggcagctgg ccgagcggcc gttcctgcag cgctcgccgg cctacgcggt
     6481 cgatgtggaa cggctgcgcg ccggcttcgg cgcctggtac gaactgttcc cgcgctctca
     6541 aagtggtgac gccggccgcc acggcacctt ccacgacgtg gtgcatcgcc tgccggcgat
     6601 caaggccatg ggcttcgacg tgctctacct gccgccgatc catcccatcg gccacacgca
     6661 ccgcaagggc cgcaacaatt cgctggtggc gcagccgggc gatcccggca gcccgtacgc
     6721 catcggcaac gagctggggg gacacgatgc catccacccg gagctcggcg gcatcgaggg
     6781 cttccggcga ctgcgccagg cctgcgcgga acacggactg gagatcgcgc tggacttcgc
     6841 catccagtgc tcgccggacc atccctggat caggcagcac ccggaatggt tttcctggcg
     6901 cagtgacggc agcctgcgcc acgcggagaa cccgccgaag aagtacgagg acatcgtcaa
     6961 cgtcgatttc tacgcgccgg gtgcgcagcc ctcgttatgg ctggcgttgc gcgacgtggt
     7021 gctgcactgg gtcggcctgg gcgtgaagat cttccgcgtc gacaacccgc acaccaagcc
     7081 gctgccgttc tggcaatggc tgatcgccga cgtgcgcgcc gcgcatcccg aggtgatctt
     7141 cctggccgag gccttcaccc gtccggccat gatgtaccgc ctcggcgaga ttggtttttc
     7201 acagagctac acctacttca cctggcgcca cagccgcgcg gaattcatcg agtacctgac
     7261 tgaactgacg caggggccgg cgcgcgattc ctaccggccc aactttttcg tcaacacgcc
     7321 ggacatcaac ccgtacttcc tgcacaactc cggccgcgcc ggcttcgtca tccgtgccgc
     7381 gctggccgcc acgctggccg gttcctgggg cctgtatagc ggcttcgagc tgtgcgaatc
     7441 ggcgccgctg ccgggcaagg aggagtatct ggactcggag aaataccagc tgcgtccacg
     7501 cgactggagt gcgcccggca acatcattgc ggagatcagc gcgctcaacc gcatccgccg
     7561 cgagcacccg gccttgcagt cgcacctgca tatgcgcttc ctgccgagca gcaatgagca
     7621 ggtgctgttt ttcgagaaga ccgcgcccgg cacggccgac cgcctgctga tcgcgatcac
     7681 gctcgaccca tggacgccgc aggatgcgtg gctcgaaatc cccgacggcg gcccctacga
     7741 ggtgcaagaa ctgatgcgcg gctaccgctt cacctggcgc ggccgcacgc agcactggcg
     7801 tttcatcccg caggaaatgc cggtcgccat ctggcagctg gaggccagcc atgccgcctg
     7861 attccggtac gcagagcctg aagcagtttg aagcgcgcgc gcgccaggac acgcactggt
     7921 acaaggacgc ggtcatctac cagctgcacg tgaagtcgtt ctacgacgcc aacaacgacg
     7981 gcgtcggcga cttcgccggc ctgatcgaga agctcgacta tatccagtca ctcggtgtca
     8041 cctgcatctg gctgctgccg ttctacccga gcccgcggcg cgacgacggc tacgacatcg
     8101 ccgagtaccg cggcatccat ccgcagtacg gctcgctggc cgacgcgcgc aggttcatca
     8161 aggaagcgca caagaggaaa ttgcgcgtga tcaccgagct ggtggtcaac cacacctccg
     8221 accagcaccc gtggttccag cgggcgcgga gg
//
LOCUS       DASZZC010000392         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_161184,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000392 DASZZC010000000
VERSION     DASZZC010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161184"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VHE37_08410"
     CDS             <1..722
                     /locus_tag="VHE37_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35592.1"
                     /translation="ITSAFGVYGDSMYAGFCGVCYIFHHGEYGVYSGIATNVNGDKPA
                     KKHSSDGWHVASAHGLPNRVINWIAIDPSDTKTVYLAIGNYSAQELAPPGSYLDATTN
                     LLSGHVYKSTDAGENFTDISGNLPDIPAISIALVNGQLLLGTQLGAFISSDTNGSSWT
                     VLGDGLPNVPVTSIVPSPADPSLVFASTYGRSIWTYKLGTAGSGASGSGGSASSRFGG
                     ALGFALLLPLTGLALLRRRRR"
     gene            811..>2120
                     /locus_tag="VHE37_08415"
     CDS             811..>2120
                     /locus_tag="VHE37_08415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT35593.1"
                     /translation="MPTQVHDPLNDGAVGVAQPIMDRSFRELFVRNPLPMWLYDKDSL
                     RFLEVNEAAVRIYGYSRAEFLSMTLADIRPKDDLPRMHNYLRRLHAEPAKPGYHRAPG
                     WRHLRKDGVLMWVDTYSHDFWYGEHLVRLVQVHDVSALKQAAERLAEQSAFFSQLFHN
                     SPEAIVMLDEHDVVVDANAAFEQLFQYTLDEIKGRGINELIVPPEHTEEATGLSRSVF
                     DRSSAQRDTVRQRKDGSLVEVSALGYPITLGSRQVGVFAIYRDITESKRLAADLAFHT
                     SHDPLTGLINRHEFERRAKARMQWGARSRVGHAMLYMDLDQFKVINDGGGHAAGDRLL
                     VEIAELLRAQLRDSDSLARLGGDEFGVLLNSVDLAQAAATAERIVHAVRDLRFKWEGR
                     VFNPGISIGVVALGGDIGTLSELMSAADTACYTAKERGRSRVQI"
BASE COUNT          345 a          760 c          679 g          336 t
ORIGIN      
        1 tcatcaccag cgcatttggt gtgtacggcg actccatgta cgccggcttc tgcggcgtct
       61 gctacatctt ccaccacggc gagtacggcg tgtattcggg catcgccacc aacgtcaatg
      121 gcgacaagcc ggcgaagaag cacagcagcg atggctggca cgtggcctcg gcgcacggcc
      181 tgcccaaccg cgtgatcaac tggatcgcca tcgatccgtc cgacaccaag acggtctatc
      241 tcgccatcgg caattactcg gcgcaggaac tggcgccgcc gggcagctac ctcgacgcca
      301 ccaccaacct gctctcgggc cacgtgtaca agtccaccga cgccggcgag aacttcaccg
      361 acatctccgg caacctgccc gacatcccgg cgatctccat cgcgctggtc aacggccagc
      421 tgctgctggg cacgcagctc ggcgcattca tttccagcga caccaacggc agcagctgga
      481 cggtgctggg cgacggcctg cccaacgtgc cggtcacctc catcgtgccc tcgccagccg
      541 atccctcgct ggtgttcgcc tccacctacg gccgcagcat ctggacctac aagctcggca
      601 ccgcgggcag cggcgccagc ggctcgggcg gctcggccag ctcgcgcttt ggcggcgcgc
      661 tcggcttcgc gctgctgctg ccgctcaccg ggctggcgct gctgcgccgg cgccggcggt
      721 aagcctcttg cgctgaagca aaggcccctg tcgtcggcac gccaggggcc ttttgcttgc
      781 ctgcacttcg caagcgatta cactgccgcc atgcctaccc aggtgcacga tccgctgaac
      841 gacggcgccg ttggcgtcgc ccagccgatc atggaccgca gcttccgcga gctgttcgtg
      901 cgcaacccgc tgccgatgtg gctctacgac aaggactcgc tgcgcttcct cgaagtgaac
      961 gaggccgcgg tgcgcatcta cggctacagc cgcgccgagt ttctgtcgat gacgctggcc
     1021 gacatccggc ccaaggatga cctgccgcgc atgcacaact atctgcgccg gctgcacgcc
     1081 gagccggcca agcccggcta tcaccgcgcg ccgggctggc ggcatctgcg caaggacggc
     1141 gtgctgatgt gggtggacac ctacagccac gacttctggt acggcgagca cctggtgcgg
     1201 ctggtgcagg tgcacgacgt gtcggcgctc aagcaggcgg ccgagcgcct ggccgagcag
     1261 agcgcgttct tctcgcagct gttccacaac tcaccggaag ccatcgtcat gctcgacgag
     1321 cacgacgtgg tggtcgacgc caacgccgcc ttcgagcagc tgttccagta cacgctggac
     1381 gagatcaagg gccgtggcat caacgagctg atcgtgccgc cggagcacac cgaggaagcc
     1441 accggcctgt cgcgcagcgt gttcgaccgc tcttccgcgc agcgcgacac cgtgcgccaa
     1501 cgcaaggacg gctcgctggt ggaagtgtcg gcgctcggct acccgatcac actgggctcg
     1561 cgccaggtgg gcgtgttcgc catctaccgc gacatcaccg agagcaagcg gctggcggcc
     1621 gacctcgcct tccacacctc gcatgatccg ctcaccgggc tgatcaaccg ccatgagttc
     1681 gagcgccgcg ccaaggcgcg catgcagtgg ggcgcgcgct cgcgcgtcgg ccacgccatg
     1741 ctgtacatgg acctggacca gttcaaggtg atcaacgatg gcggcggcca cgccgccggc
     1801 gaccgcctgc tggtggagat cgccgaactg ctgcgcgcgc agctgcgcga ctccgactcg
     1861 ctggcgcgcc tgggcggcga cgagttcggc gtgctgctca attcggtgga cctggcgcag
     1921 gccgcggcca ccgccgagcg catcgtgcac gcggtgcgcg acctgcgctt caagtgggaa
     1981 ggccgcgtgt tcaaccccgg catcagcatc ggcgtggtgg cgctgggcgg cgacatcggc
     2041 acgctgagtg agctgatgag cgcagccgat accgcctgct ataccgccaa ggaacgcggc
     2101 cgctcgcgcg tgcagatctt
//
LOCUS       DASZZC010000393         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_800793,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000393 DASZZC010000000
VERSION     DASZZC010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800793"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VHE37_08420"
     CDS             complement(<1..1037)
                     /locus_tag="VHE37_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT35594.1"
                     /translation="MNEPAPQKQRRLALIIAVVAAGLAIWGVASRIYAHVQLRERTEA
                     EAIPTVQVQTVAPAQASDQLVLPGDVQAWSDAPVYARTNGYLKRWVGDIGAKVKQGDL
                     LAEIDAPEVDQQLAQAQAELAQAQANEELARSTAERWQGLLASDSVSKQEADEKQGDY
                     QAKKALTASARANLQRLRDTQGFQKLVAPFDGTVTARNVDTGQLVGAGNAELFHVADT
                     RRLRVYAQVPQSYAQATRVGMGAQLSFADAPGKTYPATIVRTADALDPATRTLRVQLE
                     LDNAQGELLPGSYAEVHFKLGAPAQTMRLPASAPMFRGQGLQVAVVDAQNTVHVKPIT
                     VGRDFGTEVEVL"
     gene            complement(1030..>3440)
                     /locus_tag="VHE37_08425"
     CDS             complement(1030..>3440)
                     /locus_tag="VHE37_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT35595.1"
                     /translation="IRDVAHVRDGSSPQTNVVRVNGGRAVLMSVLKTGSASTLAIIDE
                     IKRRLPGVQGALPEGMTVKALGDQSIFVRAAISGVAREALIAAALTGLMILLFLGSWR
                     STLIITISIPLSILASIICLSALGETINIMTLGGLALAVGILVDDATVTIENINWHLE
                     HGKDVETAIVDGAKQIALPALVSTLSICIVFIPMFMLGGIARYLFVPLAEAVVFAMLA
                     SYLLSRTLVPTLAKYWLQAHAPHEIHGAGLWARFQNGFEQRFARLRNVYQDWLEAALA
                     ARMRFAAIFLGAMALAALLAFPIGPLPGLGQDFFPSVDSGQILLHLRARSGTRIEATT
                     MLCDEVEAAVRKAIPADELASVVDNIGLPYSGINLSYSTSAPVGPGDADIYISLSEHH
                     APTRDYVRKLRAQLPQTFPGVTFAFLPADITSQILNFGLPAPLDVQVRGFNVPGNRAY
                     LAQLYGLMRHVPGIADLRIQQAFDYPQINVDVNRSNAQQLGLTQRDVAQNLLISLAGS
                     FQTSPSFWIDPKTGSQYSVATQTPQYRETSLNDLATTPIAASAGGGTSQMLANVAGFR
                     RSFGPSVVSHYNAQPVLDIYGAVQDTDLGYVAGQVRNLVEQTAKQVPKGSTVELRGQV
                     QTMISSFNGLLIGLAAAVVLVYLLIVVNFQSWLDPFIIISALPAALAGIVWMLFLTHT
                     TVSVPSLTGAIMCMGVATANSILVVSFARERMDAGLSARDAAIEAGVTRLRPVLMTAL
                     AMIIGMVPMALSLGEGGEQNAPLGRAVIGGLSFATVATLFFVPAVFAIVHGRRAPSAG
                     VAHE"
BASE COUNT          542 a         1149 c         1211 g          538 t
ORIGIN      
        1 agcacttcca cctcggtgcc gaaatcgcgg ccgaccgtaa ttggttttac atgcaccgtg
       61 ttctgcgcat cgaccactgc cacctgcagg ccctggccgc ggaacatcgg cgcgctcgcc
      121 ggcaatcgca tggtctgcgc cggcgcgccc agcttgaagt gcacctcggc gtaggaaccc
      181 ggcagcagtt cgccctgcgc gttgtccagt tccagctgca cgcgcagcgt gcgcgtggcc
      241 ggatcgagcg cgtcggcggt gcgcacgatg gtggccggat aagtcttgcc cggcgcgtcg
      301 gcgaacgaca gttgcgcgcc catgcccacg cgcgtggcct gcgcgtagct ctgcggcacc
      361 tgcgcataca cgcgcaggcg gcgcgtgtcg gcgacgtgga acagctcggc gttgccggcg
      421 ccgaccagct ggccggtgtc gacattgcgc gcggtgacgg tgccgtcgaa cggcgccacc
      481 agtttctgga agccctgcgt gtcgcgcagc cgctgcaggt tggcgcgcgc cgaggcggtc
      541 agcgccttct tcgcctggta atcgccctgc ttctcgtcgg cctcctgctt gctcaccgaa
      601 tcgctggcca gcagcccctg ccagcgctcc gcggtgctcc tggccagttc ctcgttggcc
      661 tgcgcctgcg ccagctcggc ctgcgcctgc gccagctgct ggtccacttc cggcgcgtcg
      721 atctcggcca gcaggtcacc ctgcttgacc ttggcgccga tgtcgcccac ccagcgcttc
      781 aggtatccgt tggtgcgcgc gtagaccggc gcgtcgctcc aggcctgcac gtcgccgggc
      841 agtaccagct ggtcgctggc ctgcgccggg gccaccgtct gcacctgcac cgtggggatg
      901 gcttcggcct cggtgcgctc gcgcagctgc acgtgcgcat agatgcgcga ggccacgccc
      961 caaatggcga ggccggccgc gaccaccgcg atgatcagcg ccagccgtct ctgtttctgg
     1021 ggggcgggtt cattcatgtg caactccggc ggaaggcgcg cggcggccat gcacgatggc
     1081 gaagaccgcg ggcacgaaaa acagggtggc gacggtggcg aaggacaatc cgccgatgac
     1141 ggcgcggccc agcggcgcgt tctgctcgcc gccctcgccc agcgacagcg ccatcggcac
     1201 catgccgatg atcatggcca gcgcggtcat cagcaccggg cgcaggcggg tgacgccggc
     1261 ctcgatggcg gcatcgcgcg cggacaggcc ggcatccatg cgctcgcgcg cgaacgacac
     1321 caccagaatg ctgttggcgg tggccacgcc catgcacatg atggcgccgg tgagcgaggg
     1381 cacgctcacc gtggtgtggg tgaggaacag catccacacg atgccggcca gcgcggcggg
     1441 cagcgccgag atgatgatga acgggtcgag ccagctctgg aaattcacca cgatcagcag
     1501 gtaaaccagc accaccgcgg cggccaggcc gatgagcagg ccgttgaacg aagagatcat
     1561 ggtctgcacc tggccgcgca gttccacggt cgagcccttg ggcacctgct tcgcggtctg
     1621 ctccaccagg ttgcgcacct gcccggcaac gtagccgagg tcggtgtcct gcaccgcccc
     1681 gtagatgtcg agcacgggct gggcgttgta atggctgacc accgacgggc cgaaactgcg
     1741 ccggaagccg gcgacattgg ccagcatctg cgaggtgccg ccgccggcgc tggcggcgat
     1801 tggcgtggtc gccaggtcgt tgagcgatgt ttcgcggtac tgcggcgtct gcgtggccac
     1861 gctgtactgg ctgccggttt tcggatcgat ccagaacgac ggcgaggtct ggaagctgcc
     1921 ggccagcgag atgagcaggt tctgcgccac gtcgcgctgg gtcaggccca gttgctgcgc
     1981 gttgctgcgg ttcacatcga cgttgatctg cgggtaatcg aaggcctgct ggatgcgcag
     2041 gtcggcgatg cccggcacgt gccgcatcag gccgtacagc tgcgccaggt aagcacggtt
     2101 gcccggcacg ttgaagccgc gcacctgcac gtcgagcggc gccggcaggc cgaagttgag
     2161 gatctggctg gtgatgtcgg cgggcaggaa ggcgaaggtc acgcccggga aggtctgcgg
     2221 cagctgcgcg cgcagcttgc ggacgtaatc gcgcgtgggc gcgtggtgtt cggacagcga
     2281 gatgtagatg tcggcgtcgc ccggccccac cggcgccgag gtgctgtacg acaggttgat
     2341 gccgctgtac ggcaggccga tgttgtcgac cacgctggca agttcatcgg cgggtatggc
     2401 cttgcgcacg gcggcctcca cctcgtcgca cagcatggtg gtggcttcga tgcgcgtgcc
     2461 gctgcgcgcg cgcaggtgca gcaggatctg gccggaatcc accgacggga aaaagtcctg
     2521 gcccaggccc ggcagcgggc cgatgggaaa tgccagcagc gcggccagcg ccatcgcgcc
     2581 gagaaaaatg gcggcgaagc gcatgcgcgc ggcgagtgcg gcttcgagcc agtcctggta
     2641 gacattgcgc agccgcgcaa aacgctgctc gaagccgttc tggaagcgcg cccacaaacc
     2701 ggcgccgtga atctcgtgcg gcgcgtgcgc ctgcagccag tacttggcca gcgtcggcac
     2761 cagcgtgcgc gacagcaggt aggaggcgag catggcgaac accacggcct cggccagcgg
     2821 cacgaacagg taacgcgcga tgccgccgag catgaacatc gggatgaaca cgatgcagat
     2881 cgacagcgtg gacaccagcg ccggcagcgc gatctgcttc gcgccatcga cgatcgcggt
     2941 ctccacgtcc ttgccgtgct cgagatgcca gttgatgttc tcgatggtca cggtggcgtc
     3001 gtccaccagg atgccgaccg ccagtgccag tccgccgagc gtcatgatgt tgatggtctc
     3061 gccgagcgcc gacaggcaga tgatcgacgc caggatcgac agcgggatgg agatggtgat
     3121 gatcagcgtg gagcgccacg agcccaggaa cagcaggatc atcaggccgg tgagcgcggc
     3181 ggcgatcagc gcctcgcgtg ccacgccgga gatggcggcg cgcacgaaga tcgactggtc
     3241 gcccagcgcc ttcaccgtca tgccctcggg cagcgccccc tgcacgcccg gcaggcgccg
     3301 cttgatctcg tcgatgatgg ccagcgtcga tgcgctgccg gtcttgagca cgctcatcaa
     3361 taccgcgcgg ccgccgttga cgcgcaccac gttggtctgc ggcgaggagc cgtcgcgcac
     3421 gtgggccacg tcgcggatgt
//
LOCUS       DASZZC010000394         4404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_609670,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000394 DASZZC010000000
VERSION     DASZZC010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609670"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1805
                     /locus_tag="VHE37_08430"
     CDS             147..1805
                     /locus_tag="VHE37_08430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT35596.1"
                     /translation="MTEAKTPTIAEKLDELFKPWNRSDAPGCVVGVAREGSVLYRRAF
                     GLASIEHARANAPSVRMRIGSTTKHFCALGILLLREDGKLDIDAPVRTYLPELANIAG
                     EPTLRQLMHHTGGVRDPVASMFFLNRGTFGHAPEGSGLQLMQRFSQCNFAPGEHSAYS
                     NAGYYLLSLVIERISGQTLETYFRQRLFQPLGMHATELLRSDMAIVPNIASFHVPQAD
                     GSWRRGIYPTDEILGSGGLISTVDDMLAWAAHLRGPQRIVGNAQTWQLMLERPRLAGG
                     EPGDYCCGLARDNSRGVDLWSHAGATLGALCQMIVVPEHRIEVVVMSNRSDIPVPALA
                     MKIAEGLLEGKLQPPRVPARAEDYAALSGNRWFSPASRTLLRADRMKLPAGEALMLYL
                     NQTPAGLLFEKDGGLVRNAAAMDSLLIPQPPKGTDAPARMEVRIAGRSETFERLAQTP
                     PGAAELAPQLVGRYCYTEFGSELAIALVDGKLCLDFLPHYGRAQWELEPQSENVLTGG
                     IFHSVPPQPVPVASTVVVERESGKVRGVWLMSERFSNLWFERIS"
     gene            complement(1802..2668)
                     /locus_tag="VHE37_08435"
     CDS             complement(1802..2668)
                     /locus_tag="VHE37_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35597.1"
                     /translation="MKPNTAATHMADIGGCRLHWRVSGEPGDLPDVLLDHGGSGNADN
                     WNNLLPLLAAHTRVFAYDRAGWGESTSDGLGAGAPAVADRLSRLVAQLPIRKPYVLAG
                     YSLGGLYARYYAAQHPEQVAGLVLVDTTPTQYVIPQDKVRQAMRLIRLLHWAMRLGLG
                     RLYWYLSGRKMDAEFFRRSVSSIAARDFPARTRDELGAIPGIQQDVERVALRPQHPTL
                     AVLAGTQPKMPAEDFARLRAQHEALAQVSPPPLSRLAVVAGADHGTLLSNPAYAQEVA
                     VHMLDFARSLRR"
     gene            complement(2676..>4404)
                     /locus_tag="VHE37_08440"
     CDS             complement(2676..>4404)
                     /locus_tag="VHE37_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35598.1"
                     /translation="WSGRVSADWVHFTQGGSEPTACAMLNVPLGQSIAVRASGTFQHK
                     PGLIDIDTPGRAPQKDADDSYNRTGRVMALWQASDRLAVNLWYAQGDRKSDELPLVTN
                     AQGDFTRNDAPAPSPVDNRYALSTLDLHYAFDWATLVSVTGYQTKYSLTDTDTSYLVH
                     PLAELGLHNIHARREVHTRGVLQELRLVSPDQGPWNWLLGGFYSTYTADVSAGLYLYP
                     GLTAVDNLLSLLPPGTVPLFDPQNGFATVTNGFHPVYAYERALFGEITRTLFDDWSLT
                     LGGRWYATGVQGTPAANTKANTPLKQADNGFSPKLSVSYRPSDNLMVYTTVSRGFQYG
                     GFNVAIGLPVPLTFKSATLWNYETGLRTDWLDRTLRFDITALYLRLTNPQVYVPSNDQ
                     ISAYVDNVGGARSVGAEATLRYLTPLPGLSIEIAASHIKSTTTKEYTDADGTEVPVGS
                     AMPSAPVVQTATTLAYAHAFGSWQAQTALIHSYQGSAWNDIEHDVQVGGYSLLHASLG
                     VSRSDLPLSPSLTFSVNNLLNAHKMTSALGGADTIAADPQLSEALTSPRLRVFTVPRT
                     YRVNLQVQF"
BASE COUNT          692 a         1453 c         1561 g          698 t
ORIGIN      
        1 cagtaaacca aggctggcgg gtgggcgcga cggtgaaatc accggaagcg tcgcgcagcg
       61 gcctgcgttc cggtagtttc atggatacct gccgccgcca cggccgatgc acactgcagg
      121 ctcgaaccgc caggacctgc gcgccgatga ccgaagccaa aacgcccacc atcgcggaga
      181 agctcgacga gctgttcaaa ccctggaacc gcagcgacgc gccgggctgc gtggtcggcg
      241 tggcgcgcga gggcagcgtg ctgtaccggc gcgccttcgg cctcgccagc atcgagcacg
      301 cgcgcgccaa cgcgccatcg gtgcgcatgc gcatcggctc caccaccaag catttctgcg
      361 cgctgggcat attgctgctg cgcgaggacg gcaagctcga catcgacgcg ccggtgcgca
      421 cgtacctgcc ggaactggcc aacatcgccg gcgaacccac gctgcgccag ctgatgcacc
      481 acaccggcgg cgtgcgcgat ccggtcgcca gcatgttctt cctgaaccgc ggcactttcg
      541 gccacgcgcc cgaagggtcc ggcctgcagc tgatgcagcg cttcagccaa tgcaacttcg
      601 cgccgggcga gcattccgcg tacagcaacg ccggctacta cctgctgtcg ctggtgatcg
      661 agcgcatcag cggccagaca ctggaaacgt atttcaggca gcgcctgttc cagccgctgg
      721 gcatgcacgc caccgagctg ctgcgcagcg acatggccat cgtgccgaac atcgccagct
      781 tccacgtgcc gcaggccgac ggcagctggc gccgcggcat ctatcccacc gacgagatcc
      841 tcggctccgg cggcctgatc tccacggtcg atgacatgct ggcctgggcg gcgcacttgc
      901 gcgggccgca gcgcatcgtc ggcaatgccc agacctggca gctgatgctg gagcgcccgc
      961 gcctggccgg cggcgaaccg ggcgattact gctgcggcct ggcgcgcgac aactcgcgcg
     1021 gtgtcgacct gtggagccac gccggcgcca cgctgggtgc gctgtgccag atgatcgtgg
     1081 tgcccgagca ccggatcgaa gtggtggtga tgagcaaccg ctccgacatc ccggtgccgg
     1141 cactggcgat gaagatcgcg gaagggctgc tcgaaggaaa actgcagccg ccgcgcgtgc
     1201 cggcgcgcgc ggaggattac gccgcgctct cgggcaaccg ctggttctcg cccgcgtcgc
     1261 gcacgctgct gcgcgccgac cgcatgaaac tgcccgccgg cgaggcgctg atgctgtacc
     1321 tgaaccagac gccggcaggc ctgctgttcg agaaggacgg tggactggtg cgcaacgccg
     1381 cggcaatgga cagcctgctg atcccgcagc cgccgaaggg caccgacgct ccggcgcgga
     1441 tggaagtgcg catcgccggg cgcagcgaga cgttcgagcg cctcgcgcag acgccgcccg
     1501 gcgctgccga gctggcgccg caactggtgg gccgctattg ctacaccgag ttcggcagcg
     1561 aactcgccat cgcgctggtg gacggcaaac tctgcctcga tttcctgccg cactacggcc
     1621 gcgcgcagtg ggagctggag ccgcaatcgg aaaacgtgct gaccggcggc atcttccaca
     1681 gcgtgccgcc gcaacccgtg ccggtagcct ccaccgtggt ggtcgagcgc gagagcggca
     1741 aggtgcgcgg cgtctggctg atgtccgagc gcttcagcaa tctctggttc gagcggattt
     1801 cctagcggcg cagcgagcgc gcaaagtcga gcatgtgcac ggccacttcc tgcgcgtaag
     1861 ccgggttgct caacagcgtg ccgtgatcgg cgccggcgac gaccgcaagc cggctcagcg
     1921 gcggcggtga gacctgcgcc agcgcttcgt gctgcgcgcg caagcgcgcg aaatcttctg
     1981 cgggcatctt cggctgggtg ccggccagca ccgcgagcgt ggggtgctgc ggccgcaggg
     2041 cgacgcgctc cacgtcctgc tgaatgccgg gaatcgcgcc cagctcgtcg cgcgtgcgtg
     2101 ccgggaaatc gcgcgcggca atgctgctga cgctgcggcg gaaaaattcc gcatccatct
     2161 tcctgccgga caagtaccag tacagccggc ccaggcccag gcgcatcgcc cagtgcagca
     2221 ggcggatcag ccgcatcgcc tgccggacct tgtcctgcgg aatcacgtat tgcgtcggcg
     2281 tggtgtcgac cagcaccagg ccggcgacct gctccgggtg ctgcgcggcg tagtagcgcg
     2341 cgtagagtcc gccgagcgaa tagcccgcga gcacgtaggg cttgcggatg ggcaattgcg
     2401 ccaccagccg cgaaagccgg tcggccaccg cgggagcgcc ggcgcccagg ccgtcgctgg
     2461 tgctctcgcc ccagcccgcg cggtcgtagg caaacacgcg cgtgtgcgcg gcgagcagcg
     2521 gcagcaggtt gttccagttg tcggcgttgc cgctgccgcc gtgatcgagc aggacgtcgg
     2581 gcaaatcgcc cggttcgccg ctgacgcgcc agtgcaggcg gcagccgccg atgtcggcca
     2641 tgtgggtggc ggcagtgttg ggtttcatcg gcatgtcaga actgcacctg caggttgacg
     2701 cggtaagtcc gcggcacggt gaacacgcgc aagcgcggcg aggtcagcgc ctcggacagt
     2761 tgcggatcgg cggcaatggt atcggcgccg cccagggccg aggtcatctt gtgcgcgttg
     2821 agcaggttgt tgaccgagaa ggtcaggcta ggcgacagcg gcaggtcgct gcgcgacacg
     2881 cccaggctgg catgcagcag gctgtagccg ccgacctgga cgtcgtgctc gatgtcgttc
     2941 caggcgctgc cttgataact gtggatcagc gcggtctgcg cctgccagga gccgaaagca
     3001 tgtgcgtagg ccagcgtggt ggcggtctgc accaccggcg cgctgggcat ggcgctgccg
     3061 accgggacct cggtaccgtc cgcgtcggtg tattccttgg tggtggtgga cttgatgtgc
     3121 gaggccgcaa tttcgatcga caggcccggc agcggcgtga ggtagcgcag cgtggcctcg
     3181 gcaccgacgc tgcgcgcgcc gccgacgttg tcgacgtagg cgctgatctg atcgttgctc
     3241 ggcacgtaca cctgcggatt ggtcaggcgc aggtacagcg cggtgatgtc gaagcgcagt
     3301 gtgcggtcga gccagtcggt gcgcaggccg gtctcgtagt tccacagcgt ggctgatttg
     3361 aaggtcagcg gcaccggcag gccgatggcc acgttgaagc cgccgtactg gaagccccgc
     3421 gagacagtgg tgtagaccat caggttgtcg ctgggccggt atgacaccga cagcttgggg
     3481 ctgaagccat tgtcggcctg cttgagcggc gtgttggcct tggtgttggc ggccggcgtg
     3541 ccttgcaccc cggtggcgta ccagcggccg cccagcgtca ggctccagtc atcgaacagg
     3601 gtgcgggtga tttcgccgaa cagcgcgcgt tcgtatgcat acaccgggtg aaatccattg
     3661 gtcaccgtgg caaagccgtt ctgcggatcg aacagcggca ccgttccggg cggcagcagt
     3721 gacagcaggt tgtcgacagc ggtaaggccc gggtagagat acagacccgc ggacacgtcg
     3781 gcggtatagg tgctgtagaa gccgccgagc agccagttcc atggtccctg gtccggcgac
     3841 accagccgca gttcctgcag cacgccgcgc gtgtgcacct cgcgccgcgc gtggatgttg
     3901 tgcaggccca gttccgccag cgggtgtacg aggtaagagg tgtcggtgtc ggtcaggctg
     3961 tacttggtct ggtagccggt caccgacacc agcgtggccc agtcgaacgc gtagtgcagg
     4021 tccagcgtcg acagcgcgta gcggttgtcg accggcgagg gcgccggcgc gtcgttgcgg
     4081 gtgaagtcac cctgcgcatt ggtgaccagt ggcagctcgt cggacttgcg gtcgccttgc
     4141 gcataccaga gattcaccgc gagtcggtcg ctcgcctgcc acagcgccat cacgcggccg
     4201 gtgcggttgt agctgtcgtc ggcgtccttc tgcggcgcgc ggcccggcgt gtcgatgtcg
     4261 atcaggcccg gcttgtgctg gaaggtgccg gacgcgcgca cggcgatcga ctggcccagc
     4321 ggcacgttga gcatggcgca ggcggtcggc tcgctgccgc cctgcgtgaa gtgcacccag
     4381 tcggccgaga cgcgcccgct ccat
//
LOCUS       DASZZC010000395         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_353290,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000395 DASZZC010000000
VERSION     DASZZC010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353290"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VHE37_08445"
     CDS             <1..452
                     /locus_tag="VHE37_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35599.1"
                     /translation="PCAPAIAGSTAPGNPQCNASNQNGNTQTQDLSGKRLSYAPRWTA
                     SLIPAYQVPFDAVISTISVDVLFRGSRYLDVDLDPDKLQPATRVYNARLTLSAPSRRW
                     NFNLVAHNLTNVVFADEAISQPLAPGNVLVYRTDFGRYYSGNISLSF"
     gene            713..>1621
                     /locus_tag="VHE37_08450"
     CDS             713..>1621
                     /locus_tag="VHE37_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HVT35600.1"
                     /translation="MPSTPPSSDHRAALALAAARAAQAHGGLTLVVAANEQAAYRAEG
                     ELRFFLPREIPLLHFPDSEVLPYDQFSPHQEILSARLAALYRLPSLTRGVLLVTADAL
                     LQKLPPRAWLEGRAFMIAAGDKLDPQSFRERLVAAGYSSVSEVQTQGEFAVRGALIDL
                     FPMGSETAYRLDLFDDEIETLRTFDPETQRSQDKVSEIRLLPAREFPTDKAGIETFRR
                     RYREYFPGDPARSRIYAEVSRNLMPGGIESYLPLFFEHTATLADYLPADTQLFALDDV
                     SAALAEDWRQIEERHEQYHGDIERPLI"
BASE COUNT          268 a          576 c          514 g          263 t
ORIGIN      
        1 acccgtgcgc gccggccatc gccggctcca ccgcgccggg caacccgcag tgcaacgcca
       61 gcaaccagaa cggcaacacg cagacgcagg acctgtcggg caagcgcctg tcgtatgcgc
      121 cgcgctggac cgcttcactg atccccgcct accaggtgcc gttcgacgcg gtgatcagca
      181 ccatctcggt cgacgtgctg ttccgcggct cgcgctacct cgatgtcgat ctcgacccgg
      241 acaagctgca gccggccacg cgcgtgtaca acgcgcggct cacgctgagc gcgccgtcac
      301 gccgctggaa cttcaacctg gtggcgcaca acctgaccaa cgtggtgttc gccgacgagg
      361 cgatcagcca gccgctggcg ccgggcaacg tgctggtcta ccgcaccgac ttcggccgct
      421 actacagcgg caacatctcc ctgtcgttct agggctgctg cggcagcagg cttgcgcgca
      481 gcggatcagc gcgtcaagcc gggcagcgaa tgtgtgtcac gcgggtgcgc ccagggcgac
      541 agcccgggtc gagggtgaac gcgtatgcga taatttcgcg cctgcaaatg ctccaccgca
      601 ccgctccgtg cgatgttatt aattattgtt tgttttaata ataatacgac aataaataac
      661 atgccggaca cgggtgcggt gggcatgtcc tgagcctgtt tcctgttctc cgatgccttc
      721 cactccaccc agttccgatc accgcgccgc gctggcgctg gccgccgcgc gcgcggcgca
      781 ggcgcatggc ggcctcacgc tggtggtcgc ggccaacgag caggcggcgt atcgcgcgga
      841 aggcgagctg cgcttcttcc tgccgcgcga gatcccgctg ctgcattttc ccgacagcga
      901 ggtgctgccc tacgaccagt tctcgccgca ccaggaaatc ctgtcggcgc gcctggccgc
      961 gctgtaccgc ctgccgtcgc tgacgcgcgg cgtgctgctg gtcaccgccg atgcgctgct
     1021 gcagaagctg ccgccgcgcg cctggctcga aggccgcgcc ttcatgatcg cagccggaga
     1081 caagctcgat ccgcaatcgt tccgcgagcg cctggtcgcc gccggctaca gctcggtgtc
     1141 ggaagtgcag acgcagggcg agttcgccgt gcgcggcgcg ctgatcgacc tgttcccgat
     1201 gggctcggag accgcgtacc ggctcgacct gttcgacgac gagatcgaaa cgctgcgcac
     1261 tttcgacccg gagacgcagc gcagccagga caaggtgagc gagatccgcc tgctgccggc
     1321 gcgcgagttc cccaccgaca aggccggcat cgagaccttc cgccggcgct accgcgaata
     1381 ttttcccggc gacccggcgc gctcgcgcat ctacgccgag gtgagcagga acctgatgcc
     1441 cggcggcatc gagtcgtacc tgccgctgtt cttcgagcac accgccacgc tggccgacta
     1501 cctgccggcg gacacgcagc tgtttgcact cgacgacgtc agcgccgcgc tggcggaaga
     1561 ctggcgccag atcgaggaac gccacgagca gtaccacggc gacatcgagc ggccgctgat
     1621 c
//
LOCUS       DASZZC010000396         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_482376,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000396 DASZZC010000000
VERSION     DASZZC010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482376"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VHE37_08455"
     CDS             <1..1462
                     /locus_tag="VHE37_08455"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HVT35601.1"
                     /translation="RGVRMDWDQANGPSFYSYIASPDNTGATDGRFVQAGSTKAAIGS
                     YDYHAGVLTITVAAADLGLKGGDRITGFSAGTSLTSNIGGLPAAPVGAASPEDWMPDN
                     SARSGSYVLAANSDCTPAPDFAVALNATYSATADGSGNYPTSTDHPLHVTFTADTVGA
                     DAADLTYTFYFGDGSQSATLPAVGGAPNHYTVTHDYPYANQDGYHAYVVVTEDNATRG
                     ATSPVVSFKTSSTETVTPPPTATQAALDVSDAHPLPGNTINASSAAGTVHASGTVSSI
                     YAFDFGDGATSSVTADPATQAVVQHVYTQPGSYLVTLTLTDYDAAGGQGNVLGSSTAS
                     QSVVVGTSNATTALLAVSPSTVAVGGMVTFDGCHSFAQDGASIISYTFDAGDGSGAVE
                     QDVDAAHPVNCADGHQDASVYTHAYQAAGSYTPSLTVTDTNGTTAKRSISVGVKPAAA
                     APASAAKHGGALGLITLLPLLGFGLARRRRNSARSE"
     gene            1721..>1986
                     /locus_tag="VHE37_08460"
     CDS             1721..>1986
                     /locus_tag="VHE37_08460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HVT35602.1"
                     /translation="MVSAQDITAVLDAGLEQLSLHDARVRAQLLAFLDELQKWNAAYN
                     LTAIRDPREMVTRHLLDSLSVLPWMRGRVIDIGAGAGLPGIVLA"
BASE COUNT          366 a          706 c          618 g          296 t
ORIGIN      
        1 gcgcggcgtg cgcatggact gggaccaggc caacggaccg tcgttctatt cgtacatcgc
       61 atcgccggac aacaccggcg ccaccgacgg ccgcttcgtg caggccggtt caaccaaggc
      121 ggccatcggc agctacgact accacgccgg cgtgctcacc atcaccgtcg ctgccgccga
      181 cctggggctc aagggcggtg accgcatcac cggcttcagt gcgggcacgt cactgaccag
      241 caacatcggc ggactgccgg cggctccggt cggcgcagcg agcccggagg actggatgcc
      301 ggacaattct gcgcgcagcg gcagctacgt gctggcggcc aacagcgact gcacgcccgc
      361 accggatttc gccgtggcat tgaatgcgac ctattcggcg acggctgacg gcagcggcaa
      421 ctatccgacc agcactgatc atccgctgca cgtcaccttc accgccgaca cggtgggcgc
      481 tgacgcggcc gacctgacct acacgttcta cttcggcgac ggctcgcaga gcgcaacgct
      541 gccggccgtg ggcggtgcgc cgaaccacta cacggtgacg cacgattacc cgtacgccaa
      601 ccaggatggc tatcacgcct acgtggtggt gacggaagac aacgctacgc gcggcgccac
      661 gtcaccggtg gtcagcttca agaccagctc caccgaaacg gtgacgccgc cgccaaccgc
      721 gacgcaggcg gcgctcgacg tcagcgatgc gcatccgctg ccaggtaaca ccatcaacgc
      781 ctccagcgct gccggcaccg tgcatgcgag cgggaccgtg agcagcatct atgcgttcga
      841 cttcggcgat ggcgcaacgt ccagcgtcac cgccgatccg gccacgcagg ccgtggtgca
      901 gcatgtctac acgcagccgg gctcgtacct ggtcacgctg acgctgaccg actatgacgc
      961 ggccggcggc cagggcaatg tgctcggcag cagcacggcg tcgcagagcg tggtcgtcgg
     1021 caccagcaat gcgaccaccg cgctgctcgc ggtgtcgccg tccaccgtgg cggtcggcgg
     1081 catggtcacc ttcgacggct gccattcgtt tgcgcaggac ggcgccagca tcatcagcta
     1141 caccttcgat gccggtgacg gcagcggcgc ggtggagcag gacgtcgatg cggcgcatcc
     1201 ggtgaactgc gccgacggcc accaggacgc cagcgtctac acgcacgcct accaggcggc
     1261 gggcagctac acgcccagcc tgacggtgac cgacaccaac ggcaccacgg ccaagcgctc
     1321 gatcagcgtc ggcgtcaagc cggcggcggc cgcgccggcc agcgccgcca agcacggcgg
     1381 cgcgctgggc ctgatcacct tgctgccgct gctcggcttc ggcctggcgc ggcgcaggag
     1441 aaattccgca cgcagcgaat aactccaaga ccggcagcgc cccaccgcaa gccggcgacg
     1501 cgcctgactc ccgcctggat cggcccacac gatccaggcg ggattttttt attcaaccgc
     1561 atgccggttt atgatgttat aatttattat gtaattttaa attaaataaa taatttataa
     1621 caccaaaacc catggtgcgg tgaggcgaaa aaatggccag cacgccatcg cactcttata
     1681 ctgcgcgccc ttcagcgccg acccgagcca cgcccacgac atggtgagcg cgcaagacat
     1741 caccgccgtg ctcgacgccg gactcgagca gctttccctg cacgacgcgc gcgtgcgtgc
     1801 gcagctgctg gccttcctcg acgaactgca gaagtggaat gcggcctaca acctcaccgc
     1861 catccgcgat ccgcgggaga tggtgacccg gcacctgctc gattcgctca gcgtgctgcc
     1921 ctggatgcgc ggccgcgtga tcgacatcgg cgccggcgcc ggcctgccgg gcatcgtgct
     1981 ggccat
//
LOCUS       DASZZC010000397         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_288108,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000397 DASZZC010000000
VERSION     DASZZC010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288108"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..2097
                     /locus_tag="VHE37_08465"
     CDS             49..2097
                     /locus_tag="VHE37_08465"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HVT35603.1"
                     /translation="MQKREERAMNYAATYRRSLSDPENFWAEQAAQLHWEKKWDRVLD
                     ASTAPLHRWFTGGRLNTCFNALDRHIDGGRGEQTALIYDSPVTGTLKQYSYRELRDEV
                     ARFAGVLVAQGVRQGDRVVIYMPMVPEAAIAMLACARIGAVHSVVFGGFAPPELAKRI
                     DDAQPVVIVTASCGIEVGRVIEYKPLLDQAIDLSAHKPRRCIVLQRSQCEAALKPGRD
                     VDWNAAMRDAQPAPCASTAATDPLYVLYTSGTTGKPKGVVRDHGGHAVALAYSMRAVF
                     DTQAGDVYWAASDVGWVVGHSYIVYAPLLAGCTTILYEGKPVGTPDAGAFWRVIAQHG
                     VKTFFTAPTAFRAIKKEDPNGTLIGKYDLSKFRKLFLAGERCDPDTIHWAQEKLRVPV
                     IDHWWQTELGWPAIANCAGIELMKVKAGSATVPVPGFDLRVLDSEGAEVGAGTIGNIV
                     IKLPLPPGCLPTMWNNEQGYRENYLERYPGYYLAGDAGYVDEDGYFWVMSRIDDIINV
                     AGHRLSTGGMEEVLAGHPDVAECAVIGVADQMKGQLPVGLVVLKAGVTRAHADIVREL
                     VARVREQIGAVAAFKQVAVVARLPKTRSGKVLRATMRAIADSAPYNVPATIDDPKCLD
                     EIRTAVQALGYGKQRSYNRSHDPGPAEGTQRIAQRACFAAVADLQRDDGHCGPPSRVF
                     "
     gene            1982..>2467
                     /locus_tag="VHE37_08470"
     CDS             1982..>2467
                     /locus_tag="VHE37_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT35604.1"
                     /translation="MTRDPLKGHNALRKGRVSLPWQIYNVTTATVDRRPVFSDFDCAC
                     AASRCFARTQLLGDAAMLAWVLMPDHVHWLIQLGERDSLDLVISRLKSASAREVNRLR
                     ASSGNLWQRSFHDHALRREEDLKTAARYIVGNPVRAGLAEHVGGYPYWNAIWLQERAL
                     RA"
BASE COUNT          436 a          803 c          835 g          393 t
ORIGIN      
        1 gcgcgccttg cgcgcgatgc acgcccgcat cgcgcgcaag gcgcgctcct gcaaaagcgt
       61 gaggagagag caatgaacta cgctgcaacc taccgccgct cactgagcga tccggaaaat
      121 ttctgggccg agcaggccgc gcagctgcac tgggaaaaaa aatgggaccg cgtgctcgac
      181 gcctccaccg cgccgctgca ccgctggttc acgggcggcc ggctcaacac ctgcttcaac
      241 gcgcttgacc gccacatcga tggaggccgc ggcgagcaga ccgcgctgat ctatgacagc
      301 ccggtcaccg gcacgctgaa gcaatacagc taccgcgaac tgcgcgatga agtggcgcgc
      361 ttcgccggcg tgctggtggc acagggcgtg cggcagggcg accgcgtagt gatctacatg
      421 ccgatggtgc cggaggctgc catcgccatg ctggcctgcg cgcgcatcgg cgccgtgcac
      481 tcggtggtgt tcggcggctt tgccccgccg gaactggcca agcgcatcga cgacgcgcag
      541 ccggtggtca tcgtcacggc gtcgtgcggc atcgaagtgg gccgcgtcat cgagtacaag
      601 ccgctgctcg accaggccat cgacttgtcc gcgcacaagc cgaggcgttg cattgtgctg
      661 cagcgatccc agtgcgaagc cgcgctcaag cccggccgcg atgtggactg gaacgctgcc
      721 atgcgcgatg cgcagcctgc gccctgcgcc agcaccgcgg ccaccgatcc gctctacgtg
      781 ctctacacct ccggcaccac cggcaagccc aagggcgtcg tgcgcgatca cggcggccac
      841 gcggtggcgc tggcgtattc gatgcgcgcg gtgttcgaca cgcaggccgg cgacgtgtac
      901 tgggccgcaa gcgacgtggg ctgggtggtc ggccattcgt acatcgtcta tgcgccgctg
      961 ctcgccggct gcaccaccat cctctacgag ggcaagccgg tcggcacgcc cgacgccggc
     1021 gcgttctggc gcgtgatcgc gcagcacggc gtgaagacct tcttcaccgc gcccaccgcc
     1081 ttccgcgcga tcaagaagga agatccgaac ggcacgctga tcggcaaata cgacctgagc
     1141 aagttcagaa aactgttcct cgccggcgag cgctgcgatc ccgacaccat ccactgggcg
     1201 caggaaaaac tgcgcgtgcc agtgatcgac cactggtggc agaccgagct gggctggccg
     1261 gcgatcgcca actgcgccgg catcgagctg atgaaggtga aggccggcag cgccaccgtg
     1321 ccggtaccgg gtttcgatct gcgcgtgctc gacagcgaag gcgcggaggt tggcgccggc
     1381 accatcggca acatcgtcat caagctgccg ctgccgccgg gctgcctgcc caccatgtgg
     1441 aacaacgagc agggctaccg cgagaattat ctcgagcgtt acccgggtta ctacctcgcg
     1501 ggcgatgcgg gctacgtcga cgaggatggc tacttctggg tgatgtcgcg catcgacgac
     1561 atcatcaacg tggccgggca tcgcctgtcc accggcggca tggaagaagt gctggccggg
     1621 catcccgatg tcgccgagtg cgccgtcatc ggtgtggcag atcaaatgaa aggacagcta
     1681 cccgtcggcc tggtggtgct gaaagccggc gtcacgcgcg cgcacgcgga catcgtgcgc
     1741 gagctggtgg cacgcgtgcg cgagcagatc ggcgcggtgg cggcgttcaa gcaggtggcg
     1801 gtggtggcgc gcctgcccaa gacgcgcagc ggcaaggtgc tgcgcgccac catgcgcgcc
     1861 atcgccgaca gcgcgccgta caacgtgccg gcgacgatcg atgacccgaa gtgcctggac
     1921 gagatcagga ccgcggtgca ggcgctgggc tacgggaagc agcgctccta caataggtcg
     1981 catgacccgg gacccgctga agggacacaa cgcattgcgc aaagggcgtg tttcgctgcc
     2041 gtggcagatt tacaacgtga cgacggccac tgtggaccgc cgtcccgtgt tttctgattt
     2101 cgattgcgct tgcgccgcga gccggtgctt tgcaaggacg cagcttttgg gcgatgcggc
     2161 aatgctggcg tgggtgctga tgcctgacca tgtgcactgg ctgattcagc tgggcgagcg
     2221 tgattcgctc gatctggtca tcagccgttt aaagtccgcc agcgcacgcg aggtgaatcg
     2281 cctgcgagcc agtagcggaa acctgtggca gcgctcattc cacgaccatg ctctgcgcag
     2341 ggaagaggat ctgaaaactg ctgcacgcta catcgtgggt aatcccgtgc gtgccgggct
     2401 ggccgagcat gtcggcggct acccctactg gaacgcgatc tggctgcagg agcgcgcctt
     2461 gcgcgcg
//
LOCUS       DASZZC010000398         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_998682,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000398 DASZZC010000000
VERSION     DASZZC010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998682"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VHE37_08475"
     CDS             complement(<1..442)
                     /locus_tag="VHE37_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35605.1"
                     /translation="MPLVTLPSGQLDYALEGAGAPLVLLSANPGDRRDFDAVAPELAQ
                     RYQLLRLDWPGYGLSPAPQPPASASAMLFAQLLGELMQQLQLPPAIILGNSVGCYAAV
                     SLALAQPAQVRALVLVSPGGFSPQNAVTRTFCRIKGNEAVTRATN"
     gene            complement(684..>2056)
                     /locus_tag="VHE37_08480"
     CDS             complement(684..>2056)
                     /locus_tag="VHE37_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVT35606.1"
                     /translation="LPALIALLPPDAERVKLTPSTDGWLGRALAWPYTYSRQIWIGAA
                     LLAVGSLALAPRARFDYDPINLRDPKAESVATFRDLVRDPNIPSLALSAVAPDAKSAQ
                     QLSDQFGQLPLVRRAMSLNDLVPKDQDAKLAIIQDLSFTLGPALSGSGKVQLRANDAD
                     DRAAIEALRASLPAYIKTQGGTRAARSQALLDALDRFHAELAKRDAAGQAKLLAALRE
                     DLLSAIPAHLEDLKMSLQADHVGENDLPPNMLASWKSSDGHYRVEVWPKEILDTPAAI
                     EHFVDQVRGAYPEAVGGPRGMMEAGRAVVAAFKTAFVYSLIAITVLLLVLLRSVGDTV
                     LVLIPLTLAGLLTLAGTVLFNIPFNFANVIALPLILGVGVDYGVYLVQRGRAAGAQVN
                     ILQTSTARAVLYGALITMANFGNLALAKHPGMVSMGLLLTIGLGMTLVCALVLLPSLL
                     ARRRSA"
BASE COUNT          351 a          690 c          712 g          303 t
ORIGIN      
        1 cattggtagc gcgcgtaacc gcctcgttgc ccttgatgcg gcagaaggtg cgcgtcaccg
       61 cgttctgcgg cgagaagccg ccgggcgaca ccagcaccag cgcgcgcacc tgcgccggct
      121 gcgccagcgc gaggctgacc gcggcgtagc agcccaccga gttgcccagg atgatcgccg
      181 gcggcagctg cagctgctgc atcagttcgc cgagcagctg cgcgaacagc atggcgctgg
      241 ccgaggccgg cggctgcggc gccggcgaga gtccatagcc gggccagtcc agccgcagca
      301 gctggtagcg ctgcgccagt tcgggcgcca ccgcgtcgaa gtcgcggcgg tcgcccggat
      361 tggccgacag cagcaccagc ggcgcgccgg caccttccag cgcatagtcc agctggcccg
      421 aaggcagggt gacgagaggc atgcgtcgct ccaatgactg accggtcagt cagtgaacga
      481 tacgcgcatg ccggcgacgg atcaagccgg ttgcgccagc ccgccatgtc cgctcccagg
      541 atgccggaat ggcgctccgg cgtggcaaat cccgtctcac cgcatgccgg gtttatgtgt
      601 tacgaaaaat taaatatttt tataattgaa taataattta taacataaac aatgcaggtg
      661 cggtggccgg ctttttgcgc gtgtcaggca ctccggcgcc gcgccagcag gctgggcagc
      721 agcaccagcg cgcacaccag cgtcatgccc aggccgatgg tcagcagcag gcccatgctg
      781 accatgccgg gatgcttggc cagggcgagg ttgccgaagt tggccatggt gatcaacgcg
      841 ccgtagagca ccgcgcgcgc ggtggaggtc tgcaggatgt tgacctgcgc gccggccgcg
      901 cggccgcgct gcaccaggta cacgccgtag tccacgccca cgcccaggat cagcggcagc
      961 gcaatcacgt tggcgaagtt gaacgggatg ttgaacagca cggtgcccgc cagcgtgagc
     1021 aggccggcca gcgtgagcgg gatcagcacc agcacggtgt cgcccacgct gcgcagcagc
     1081 accagcagca acacggtgat ggcgataagc gaatagacga aggcggtctt gaacgcagcc
     1141 accaccgcac ggccggcttc catcatgccg cgcgggccgc cgaccgcttc cgggtacgcg
     1201 ccgcgcacct ggtcgacgaa atgctcgatc gccgccggcg tgtcgaggat ttccttcggc
     1261 cacacctcga cgcgatagtg gccgtcgctg ctcttccagc tggccagcat gttcggcggc
     1321 aggtcgttct cgcccacgtg atcggcctgc agcgacatct tcagatcctc gagatgcgcg
     1381 ggaatggccg agagcagatc ctcgcgcagc gccgcgagca gcttcgcctg ccccgcggcg
     1441 tcacgctttg ccagctcggc gtggaagcgg tcgagtgcat cgagcagcgc ctgcgagcgc
     1501 gccgcgcgtg tgccgccctg cgtcttgatg tacgccggca gcgacgcgcg cagcgcttcg
     1561 atggctgcgc gatcgtcagc gtcattggcg cgcagctgca ctttgccgct gccggacagc
     1621 gccgggccga gcgtgaacga caggtcctgg atgatggcga gcttcgcgtc ctggtccttc
     1681 ggcaccaggt cgttgaggct catcgcgcgg cgcaccagcg gcagctggcc gaactggtct
     1741 gacagctgct gcgccgactt cgcgtccggc gcaacggcgg acaaggccag cgacggaatg
     1801 ttgggatcgc gcaccaggtc gcggaaggtg gccacggact cggccttggg atcgcgcagg
     1861 ttgatcgggt cgtagtcgaa gcgcgcgcgc ggcgccagcg ccagcgaacc gacggcgagc
     1921 agcgcggcgc cgatccagat ctggcgcgag tacgtgtacg gccaggccag cgcacggccg
     1981 agccagccgt cggtggacgg cgtcagcttc acgcgctcgg catccggcgg cagcagcgcg
     2041 atcagcgccg gcagca
//
LOCUS       DASZZC010000399         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_353820,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000399 DASZZC010000000
VERSION     DASZZC010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353820"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..436)
                     /locus_tag="VHE37_08485"
     CDS             complement(107..436)
                     /locus_tag="VHE37_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35607.1"
                     /translation="MSESSIDGQIIDRRLAIEVLHQAQVALPQPIRGWIAASGKFIAG
                     EAPAAVAPWARLWSHPQAPAVPAAGELAPGVLNLVISLNTKGVLEMRAWRLVDGAPAE
                     VVLKIRE"
     gene            complement(433..1158)
                     /gene="moeB"
                     /locus_tag="VHE37_08490"
     CDS             complement(433..1158)
                     /gene="moeB"
                     /locus_tag="VHE37_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HVT35608.1"
                     /translation="MSTFARYSRQVLLREVGVNGQALLRDSSVLIVGAGGLGSAAALY
                     LAGAGVGRLVLADRDRVELSNLQRQILFRHADVGRGKTAAAATALAAFNPEVQVEVRD
                     GDSWRDAVHHCDVVLDCTDNFPTRFAINAACVSARNPLVSGAAIRLEGQLAVFDRARG
                     TACYACLYPDAGEAQETCEEAGILGPVAGTIGAMQALCALKLLLGIGDDRGRLHLWDA
                     RTLAWRTSTMTRDPDCSCCRSAT"
     gene            complement(1155..1940)
                     /gene="prmC"
                     /locus_tag="VHE37_08495"
     CDS             complement(1155..1940)
                     /gene="prmC"
                     /locus_tag="VHE37_08495"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVT35609.1"
                     /translation="MHEQLPEAVTRAEREWLRAHVLGTTRTRAAAQALDPRQQGEFDA
                     LLARRAAGEPLAYILGEWEFWSLPLKITPDVLVPRADTETLVEWALQLAPPQARVLDL
                     GTGSGAIGLALARERPQASVVAIDTSTAALEVAQHNAQRLGIANVEFLHTDFRAALDD
                     GERYELVVSNPPYIAENDPHLADLQYEPRIALTAGADGLDQLRVIVANAPAALEERGW
                     LLVEHGYDQGAAVRGLFERAGFLQVQTRRDLGGNERVTGGRRA"
     gene            complement(1913..2308)
                     /locus_tag="VHE37_08500"
     CDS             complement(1913..2308)
                     /locus_tag="VHE37_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35610.1"
                     /translation="MKYLMVLSLLALAAAACTPAYQDQYDYQPPHGEVAQSCVAQCSV
                     TRDLCVASFRSADGSGRQQCEVQSNQEMAHCQGRATNGAEAGLCTPRSCNEPTAAATP
                     CDDEYRVCYASCGGTVTRRQVCTSNCPKP"
     gene            complement(2305..3384)
                     /gene="prfA"
                     /locus_tag="VHE37_08505"
     CDS             complement(2305..3384)
                     /gene="prfA"
                     /locus_tag="VHE37_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HVT35611.1"
                     /translation="MKPSLLAKLQQMAERREEVARELSQPGVDAEKFRRLSQEYAQLG
                     PLAENYAAHAAALAERGNLEQMQSDPDRELREMAVDDVARLERRLRDLDGELQLLLLP
                     KDPRDEANLYLEVRAGTGGDEAAIFAGDLARMYAKYAEARGWQLEPISHSEGEHGGYK
                     EFIARIAGYGAYSQLKFESGAHRVQRVPETEAQGRIHTSAATVAVLPELEEAQIEINP
                     ADLKFDTFRSSGAGGQHVNKTESAIRVTHIPTGVVVECQEERSQHKNRAKALALLASR
                     LNDATRSRQQTEMAATRRKLVGSGDRSERIRTYNFPQNRVTDHRINLTLYQLERVMQG
                     DLDPVIQPLLAEHQAELLAEAGATA"
     gene            complement(3476..4735)
                     /gene="hemA"
                     /locus_tag="VHE37_08510"
     CDS             complement(3476..4735)
                     /gene="hemA"
                     /locus_tag="VHE37_08510"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HVT35612.1"
                     /translation="MTLLTLGLSHHAAPLDARERLAFTDADLPAALMRLRALPGVDEA
                     AIVSTCNRTEILAVTSLEEEARLVEWWRRERGAAEGIIEKFSYLHRDLGSVTHSLRVA
                     SGLDSMVVGEPQILGQMKHSYTVASEQRALGPVLSRLFQHAFAVAKVVRSKTEIGAHP
                     VSIAYAAVQMARRIFGDLSGQSALIIGAGDHARLIAQHLAAQGVARIVVANRNLERAE
                     ALAREVRGYAVTLADMPAHLAEAGLVIASTGARGYMLDREAVQRAIGRRRRKPVFMID
                     LAVPRDIAPDVAQIEDVYLYTVDDLRSVIEENLKLRGEAAQQAEVLVREYALEFNRWL
                     ESRNSAATIAALRQHGRAYRDEVLEKARRRLAAGEAPAEVLTWMADTLSNKLLHAPSA
                     ALRSADAVEQALLLSAARKLFDLPDEN"
     gene            5004..>6619
                     /locus_tag="VHE37_08515"
     CDS             5004..>6619
                     /locus_tag="VHE37_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT35613.1"
                     /translation="MLPTSPRLACAAALTLLAAACATPRQSPAMVAGADDAPLPVKPE
                     DARTTGTLPPAPPEIIAPPVRTLSASQASSVELHVMTGEMAADRRQPELAAKEFLEAL
                     KLVDDADLAQRATLLAASAHDEDLALSAARRWLQISPNDGEPREVIARVEISRGHLKE
                     TYEQLEAIVSGAAGGPEDGFHQAAVILSMAGKEHADAAIAVMKMLVNKWPQLPAAHRS
                     LGLLALRYNDLPLADGAAREALKLAPDSREDSLLLAGVLVRQGKVAESDAIIDKLAAS
                     DKSSADLRLGYAKLLLENDQREQARTQLQKMLQADPNNVDARYALGVLAVNDHNDADA
                     EKFLLPLLDTARANEAAFQLGKLEENDKHYDKALGYYERVNSGASALDAAVRRAGIMG
                     ETGHMKDGRDLLAELRDQFPQLSSRLWLAEGDMLTNAGQFGEAMQVYNTALMESPDDP
                     DLLYGRSLVYEKQGKTDLAEKDLRSLIKKDPDDARAMNALGYLLVVHTQRFDEASKLI
                     GRALELEPDDAAILDSMGWVQYKLGHTSEAR"
BASE COUNT         1018 a         2335 c         2243 g         1023 t
ORIGIN      
        1 cagctcggca ttggtgcggc ggccgccgaa ctgtgaaatg cggtcgcgcg ccggatcggt
       61 gccgaaagtc tcgtagtcgt gccagtagaa cgtctcgttc acgcgcctac tcgcggatct
      121 tcaggaccac ttccgccggc gcgccgtcga ccagccgcca cgcgcgcatc tccagcacgc
      181 ccttggtgtt gagcgaaatg accaggttga gcacgccagg cgcgagctcg ccggcagcgg
      241 gcacggccgg cgcctgcggg tgcgaccaca gcctagccca cggcgcgacc gcggcgggcg
      301 cttcgccggc gatgaatttc ccggacgccg cgatccagcc gcggatcggt tgcggcagcg
      361 ccacctgggc ctggtgcagc acctcgatgg ccaggcggcg gtcgatgatt tgtccgtcga
      421 tagatgactc gctcacgtcg cactccggca gcaggaacag tcgggatcgc gcgtcatcgt
      481 actcgtgcgc cacgccagcg tgcgcgcgtc ccacaggtgc aggcggccgc gatcgtcgcc
      541 gatgcccagc agcagtttca gcgcgcacaa ggcctgcatg gcgccgatgg tgccggccac
      601 cggaccgagg atgccggcct cctcgcaggt ttcctgcgcc tcgcccgcat ccgggtacag
      661 gcaggcgtag cacgccgtgc cgcgcgcgcg gtcgaacacc gcgagctgcc cttcgagccg
      721 gatggccgcg cccgacacca gcggattgcg cgccgacacg caggccgcgt tgatggcaaa
      781 gcgcgtgggg aagttgtccg tgcagtcgag cacgacatcg cagtgatgca ccgcatcgcg
      841 ccagctgtcg ccatcgcgca cttccacctg cacctcggga ttgaacgccg ccagcgcggt
      901 ggccgcagcg gcggtcttgc cgcggccgac atcggcgtgg cggaacagga tctgccgctg
      961 caggttggac agttccacgc ggtcgcggtc ggccagcacc agccggccga cgccggcgcc
     1021 ggcgagatac agcgcggccg cgctgcccag cccgccggca cctacgatca gcacgctgga
     1081 atcgcgcagc agcgcttgcc cgttcacgcc cacttcgcgc agcagcacct ggcgtgaata
     1141 gcgcgcgaac gtactcatgc acgcctcccg ccggtcacgc gctcgttgcc gccgagatcg
     1201 cgccgcgtct gcacctgcag gaatcccgcg cgctcgaaca atccgcgcac ggcggcgccc
     1261 tggtcgtagc catgctccac cagcagccag ccgcgctctt ccagtgcagc cggcgcgttg
     1321 gccacgatca cgcgcagctg gtcgagaccg tcggcgccgg cggtgagcgc gatgcgcggc
     1381 tcgtattgca ggtcggccag gtgcggatcg ttctcggcga tgtagggcgg attcgacacc
     1441 accagctcgt agcgctcgcc atcatccagc gccgcgcgaa aatcggtgtg aaggaattcc
     1501 acgttggcga tgcccaggcg ctgcgcgttg tgctgcgcca cttccagtgc agcggtcgag
     1561 gtatcgatgg ccaccacgct cgcctgcgga cgctcgcgcg ccagcgccag tccgatggca
     1621 ccgctgccgg ttcccaggtc cagcacgcgc gcctgcggcg gcgccagctg caatgcccat
     1681 tcgaccagcg tctcggtatc ggcgcgcggc accagcacgt cgggcgtgat cttcagcggc
     1741 agtgaccaga attcccattc gccgaggatg taggccagcg gctcgccggc cgcgcggcgc
     1801 gccagcagcg catcgaactc gccttgctgc cgcgggtcga gcgcctgcgc cgccgcgcgc
     1861 gtgcgcgtgg tgcccagcac gtgcgcgcgc aaccactcgc gctcagcgcg cgttacggct
     1921 tcgggcagtt gctcgtgcat acctgccggc gcgtcaccgt gccgccgcag ctggcgtagc
     1981 agacgcggta ttcgtcgtcg cagggcgtcg ccgcggcggt cggctcgttg cagctgcgcg
     2041 gcgtgcacag gccggcttcg gcgccgttgg tggcgcgccc ctggcaatgc gccatctcct
     2101 ggttcgattg cacctcgcac tgctggcggc cgctgccgtc ggcgctgcgg aagctggcga
     2161 cgcacaggtc tcgggtcacg gagcactggg ccacgcagga ctgcgccact tcgccgtgcg
     2221 gcggctggta gtcgtactgg tcctggtacg cgggcgtgca cgccgcggcg gccagcgcca
     2281 gcagcgacaa caccatcagg tatttcatgc ggtagctccg gcttcggcca gcaattctgc
     2341 ctgatgctcc gccagcaacg gctggatcac cgggtccagg tcgccctgca tcacccgctc
     2401 gagctggtac agcgtgagat tgatgcgatg atcggtcacg cggttctgcg gaaagttgta
     2461 ggtgcggatg cgctcgctgc ggtcgccgct gcccaccagc ttgcgccgcg tggccgccat
     2521 ctcggtctgc tgcctgctgc gggtggcatc gttcagccgc gaggccagca gcgccagcgc
     2581 cttggcgcgg ttcttgtgct ggctgcgctc ttcctggcac tccaccacca cgccggtagg
     2641 tatgtgcgtg acgcggatgg ccgactcggt cttgttcacg tgctggccgc cggcgccgga
     2701 ggaacggaag gtgtcgaact tgagatcggc cggattgatc tcgatctgcg cctcttccag
     2761 ttccggcagc accgccacgg tggccgcgct ggtgtggatg cgcccctgcg cctcggtttc
     2821 cggcacgcgc tgcacgcggt gcgcgccgct ctcgaacttg agctgcgaat aggcgccgta
     2881 gccggcgatg cgcgcgatga actccttgta gccgccgtgc tcgccctcgc tgtgcgagat
     2941 cggctccagc tgccagccgc gcgcttccgc gtacttggcg tacatgcgcg caaggtcgcc
     3001 ggcgaagatc gccgcctcgt cgccgccggt gccggcgcgc acctccaggt acaggttggc
     3061 ctcgtcgcgc gggtccttcg gcagcagcaa cagctgcagc tcgccgtcca gatcccgcag
     3121 gcgccgttcc agccgcgcca cgtcgtcgac ggccatctcg cgcagttcgc gatcggggtc
     3181 ggactgcatc tgctccaggt tgccacgttc ggccagcgcc gccgcatgcg cggcatagtt
     3241 ctcggccagc ggcccgagct gcgcgtattc ctgcgacagg cggcggaatt tctcggcgtc
     3301 gacgccgggc tgcgacagct cgcgcgccac ttcctcgcgg cgctcggcca tctgctggag
     3361 cttggctagc aggctgggtt tcatcgatgg aatgggtgat cagcgtggaa ctgaggaact
     3421 gaattggcgg gaaggacgaa agcggccgcc ggccgcgcgc cgcggtgcgc ggtcgctaat
     3481 tctcgtcggg caggtcgaac agcttgcgcg ccgccgacag cagcagggcc tgttccaccg
     3541 catcggcgct gcgcagcgct gccgagggcg cgtgcagcag cttgttggac agcgtgtcgg
     3601 ccatccaggt gagcacctcg gccggcgcct cgccggcagc gagacggcgg cgcgcttttt
     3661 ccagcacctc gtcgcggtag gcacgcccgt gctgcctcag cgcagcgatc gtcgcagcgg
     3721 agttgcgcga ttccagccag cggttgaatt ccagcgcgta ctcgcgcacc agcacctcgg
     3781 cctgctgggc tgcctcgccg cgcagcttga ggttctcttc gatcaccgag cgcaggtcat
     3841 cgaccgtgta caggtacacg tcctcgatct gcgccacgtc gggcgcgatg tcgcgcggca
     3901 ccgccaggtc gatcatgaac accggcttgc gccggcgcct gccgatggcg cgctgcaccg
     3961 cctcgcggtc gagcatgtag ccgcgcgcgc ccgtggaggc gatgaccagc ccggcctcgg
     4021 ccaggtgcgc cggcatgtcg gccagcgtca ccgcgtagcc acgcacctcg cgcgccagcg
     4081 cctcggcacg ctcgaggttg cggttggcca ccacgatgcg cgccacgccc tgcgccgcca
     4141 ggtgctgcgc gatcagccgc gcgtggtcgc cggcgccgat gatcagcgcg ctctgcccgg
     4201 acaggtcgcc gaagatgcgc cgcgccatct gcaccgcggc ataggcgatc gacaccgggt
     4261 gcgcgccgat ctcggtcttc gagcgcacca ccttggccac ggcgaaggcg tgctggaaca
     4321 ggcgcgacag caccgggccg agcgcgcgct gctcgctggc cacggtgtac gaatgcttca
     4381 tctggccgag gatctgcggc tctcccacca ccatcgagtc gaggcccgaa gcgacgcgca
     4441 ggctgtgcgt cacgctgccc aggtcgcggt gcaggtagga gaatttctcg atgatgcctt
     4501 cggccgcgcc gcgctcgcgc cgccaccact cgaccagccg cgcctcttcc tccagcgagg
     4561 tcaccgcgag gatttccgtg cggttgcagg tggagacgat ggccgcttcg tcgacacccg
     4621 gcagcgcgcg cagccgcatc agcgcggcgg gcaggtcggc gtcggtgaag gccagccgct
     4681 cgcgcgcgtc cagcggcgcg gcatgatgcg acaggcccag ggtgagcagc gtcatgtgca
     4741 aattttccct gctgcgacga tcagcgacaa gtcggaagct ggatgagagt gcatctcgat
     4801 ggccgcaagc ggcgtgccgg tggcttaccg caagggcgtt tttgatgtta tttaatataa
     4861 tactatttta aatatagaca atattacata acacactaga tcggcttgcg gtgaggcatg
     4921 cagacgcgcg ggacccggtg tgcaggaacc gcgctatcat ccccgcggtc ttaaaaatcc
     4981 ccgtcttctc tccaaggttt cccgtgctgc ccacctcccc gcgcctggcc tgtgccgctg
     5041 ccttgaccct gctcgctgct gcctgcgcca cgccgcgcca gtcgccggcg atggtcgccg
     5101 gggccgacga cgcgccgctg ccggtgaagc ccgaggatgc gcgcaccacc ggcacgctgc
     5161 cgccggcgcc gccggagatc atcgcgccgc cggtgcgcac gctcagcgcg tcgcaggctt
     5221 cctcggtgga actgcacgtg atgaccggtg aaatggctgc cgaccgccgc cagccggaac
     5281 tggcggcgaa ggaattcctg gaagcgctca agctggtcga cgacgccgat ctcgcccagc
     5341 gcgccacgct gctcgccgcc agcgcgcacg acgaggatct cgcgctcagc gccgcgcggc
     5401 gctggctgca gatctcgccc aacgacggcg agccgcgcga ggtgatcgcg cgcgtcgaga
     5461 tcagccgcgg acacctgaag gaaacctacg agcagctgga agcgatcgtc agcggcgcgg
     5521 ccggcggacc ggaagatggc ttccaccagg ccgcggtgat cctgtcgatg gccggcaagg
     5581 agcacgccga cgccgccatc gcggtgatga agatgctggt caacaagtgg ccgcagctgc
     5641 cggcggcgca ccgctcgctc ggcctgctgg cgctgcgcta caacgacctg ccgctggccg
     5701 acggcgcggc gcgcgaggcg ctgaagcttg cacccgacag ccgcgaggat tcgctgctgc
     5761 tggccggcgt gctggtgcgc cagggcaaag tggcggaatc cgacgccatc atcgacaagc
     5821 tggccgccag cgacaaatcg tccgccgacc tgcgcctggg ctacgccaag ctgctgctgg
     5881 aaaacgacca gcgcgagcag gcgcgcacgc agctgcagaa gatgctgcag gccgatccga
     5941 acaatgtcga tgcgcgctac gcgctcggcg tgctggcggt gaacgaccac aacgatgccg
     6001 atgcggaaaa attcctgctg ccgctgctcg acaccgcgcg cgccaacgag gccgccttcc
     6061 agctggggaa gctggaagag aacgacaagc actacgacaa ggcgctgggc tactacgagc
     6121 gcgtgaacag cggcgcctcg gcactcgacg ccgccgtgcg ccgcgccggc atcatgggcg
     6181 aaaccggcca catgaaagat ggccgcgacc tgctggccga actgcgcgac cagtttccgc
     6241 agctgtcctc gcgcctgtgg ctggctgaag gcgacatgct gaccaacgcc ggccagttcg
     6301 gcgaagccat gcaggtgtac aacaccgcgc tgatggaatc gcccgacgat cccgacctgc
     6361 tctacggccg ctcgctggtc tacgaaaagc agggcaagac cgacctggcg gaaaaggact
     6421 tgcgctccct gatcaagaag gaccccgacg atgcgcgcgc gatgaatgcg ctgggctacc
     6481 tgctggtggt gcacacgcag cgcttcgatg aagccagcaa gctgatcggc cgggcgctgg
     6541 aactggaacc ggacgacgcc gccatcctcg acagcatggg ctgggtgcaa tacaagctcg
     6601 gccataccag cgaggcgcg
//
LOCUS       DASZZC010000400         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_94319,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000400 DASZZC010000000
VERSION     DASZZC010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94319"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /gene="purB"
                     /locus_tag="VHE37_08520"
     CDS             <1..1278
                     /gene="purB"
                     /locus_tag="VHE37_08520"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HVT35614.1"
                     /translation="GLMRYRVLVEIRWLQALAAHAGVPEVKPLSPSAHERLSKIAENF
                     DIGEAQRIKTIEDTTNHDVKAVEYYLKERIATHEELAAIREFIHFACTSEDVNNVAHA
                     LMLKDARDKVLLPQIDKLIAAISSLAHSYAEQPMLARTHGQPASPTTLGKEMAVFAQR
                     LKRQRKQLAQVELLAKLNGAVGNYNAHLAAYPDVDWSALGDSVLQQLGVQRSPATTQI
                     EPHDYIAEYFHALSRINTILIDFARDIWGYVSLGYFKQRTIAGEVGSSTMPHKVNPID
                     FENGEGNLGVANAILQHLAEKLPISRWQRDLTDSTVLRNLGVGVAHSLVAYQAIGKGI
                     GKLEADAWKMGQDLEANWEVLGEAIQTVMRRHGLPEPYEQLKRLTRGQKIGRDAIRDF
                     VEKLPLPEAEKKRLLAMTPATYTGNAMAQAKDV"
     gene            1278..1790
                     /locus_tag="VHE37_08525"
     CDS             1278..1790
                     /locus_tag="VHE37_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35615.1"
                     /translation="MSPGAPGTDDTAPRYHIISGAAAYAQHLRELAAAAHVSMLIFTH
                     DLDQRIYGGEEFIDALKKFILQNRRANLRVIIHSPGLAMRRGHRLVELGRALSSRIEF
                     RELPEQYQSLIEEYAIADQRGGLYKEYYGDLESQWHEHDPLFARERLRAFDTLWPECV
                     PARELRDLRL"
     gene            complement(1925..>2254)
                     /locus_tag="VHE37_08530"
     CDS             complement(1925..>2254)
                     /locus_tag="VHE37_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT35616.1"
                     /translation="MGIGIAIDDFGTGYSSLAYLSKLPVNAVKIDRSFIINMTDSADT
                     MGIVSTIISMAHAMHLKVVAEGVDSHEQLKFLRLLRCDEIQGYLYSKALPEAEFAELL
                     REGRMLA"
BASE COUNT          427 a          755 c          725 g          347 t
ORIGIN      
        1 gggctgatgc gctaccgcgt gctggtggag atccgctggc tgcaggcgct ggccgcgcat
       61 gccggcgtgc ccgaggtgaa gccgctgtcg ccgtcggcgc acgagcgcct gtcgaagatc
      121 gcggagaact tcgacatcgg cgaagcccag cgcatcaaga ccatcgagga caccaccaac
      181 cacgacgtca aggcggtgga gtattacctg aaggagcgca tcgccacgca cgaggaactg
      241 gcggccatcc gcgagttcat ccacttcgcc tgcacctcgg aagacgtcaa caacgtggcg
      301 catgcgctga tgctgaagga cgcgcgcgac aaggtgctgc tgccgcagat cgacaagctg
      361 atcgccgcga tcagttcact ggcgcacagc tacgccgagc agccgatgct ggcgcgcacc
      421 cacggtcagc cggcctcgcc caccacgctg ggcaaggaga tggcggtgtt cgcgcagcgc
      481 ctcaagcgcc agcgcaaaca gctggcgcag gtggaactgc tggccaagct caacggcgcg
      541 gtgggcaact acaacgcgca cctggcggcg taccccgacg tcgactggtc cgcgcttggc
      601 gacagcgtgc tgcagcagct cggcgtgcag cgcagcccgg ccaccacgca gatcgagccg
      661 cacgactaca tcgccgagta cttccacgcg ctgtcgcgca tcaacaccat cctgatcgat
      721 ttcgcgcgcg acatctgggg ctacgtctcg ctgggctact tcaagcagcg caccatcgcc
      781 ggcgaagtgg gcagcagcac catgccgcac aaggtcaacc cgatcgactt cgaaaacggc
      841 gaaggcaacc tgggcgtggc caacgccatc ctgcagcacc tggccgagaa gctgccgatc
      901 tcgcgctggc agcgcgatct caccgattcc accgtgctgc gcaacctggg cgtgggcgtg
      961 gcgcactcgc tggtcgccta ccaggccatc ggcaagggca tcggcaagct cgaggccgac
     1021 gcctggaaga tggggcagga tctcgaggcc aactgggaag tgctgggcga agcgatccag
     1081 acggtgatgc gccgccacgg cctgcccgag ccgtacgagc agctgaagcg cctgacgcgc
     1141 gggcagaaga tcggccgcga cgccatccgc gacttcgtcg agaagctgcc gctgccggaa
     1201 gccgagaaga agcgcctgct ggcgatgacg ccggccacct ataccggcaa cgccatggcg
     1261 caggccaagg acgtgtgatg tctcccggcg cgccaggcac cgacgacacc gcgccgcgct
     1321 accacatcat ctccggcgcg gcggcctacg cgcagcacct gcgcgagctg gccgcagcgg
     1381 cccacgtgtc gatgctgatc ttcacgcatg acctggacca gcgcatctac ggcggcgagg
     1441 agttcatcga cgcgctgaag aaattcatcc tgcagaaccg ccgcgccaac ctgcgcgtga
     1501 tcatccactc gccgggcctg gccatgcgcc gcggccaccg cctggtggaa ctgggccgcg
     1561 cgctgtccag ccgcatcgag ttccgcgagc tgcccgagca gtaccagtcg ctgatcgagg
     1621 aatacgccat cgccgaccag cgcggcggcc tgtacaagga gtactacggc gacctcgaat
     1681 cgcagtggca tgagcacgac ccgttgttcg cacgcgagag gctgcgcgcg ttcgacacgc
     1741 tgtggccgga gtgcgtgccg gcgcgcgagt tgcgcgatct caggctctag gcggccgctt
     1801 cacggggtca ccgcaccccc cttttgcttg ttattaatta ttatttaatt tcaactatag
     1861 gcaataatta ataacaccgg cgttgccggt gcggtggagc ttcccgggct ctccgggaag
     1921 cccgctaggc cagcatgcga ccctcgcgca ggagctccgc gaattcagct tccggcagcg
     1981 ccttgctgta caggtatccc tggatctcgt cgcagcgcag cagccgcagg aacttgagct
     2041 gctcgtgcga gtcgacgccc tccgccacca ccttgagatg catggcgtgc gccatcgaga
     2101 tgatggtgga gacgatgccc atggtgtcgg cgctgtcggt catgttgatg atgaaggaac
     2161 ggtcgatctt gaccgcgttc accggcagct tggacaggta cgcgagtgac gaatagccgg
     2221 tgccgaaatc gtcgatggcg atgccgatgc ccat
//
LOCUS       DASZZC010000401         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_353884,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000401 DASZZC010000000
VERSION     DASZZC010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353884"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3618
                     /locus_tag="VHE37_08535"
     CDS             <1..3618
                     /locus_tag="VHE37_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT35617.1"
                     /translation="LKETLNTMVDQLNRFAGEVTRVAREVGTEGRLGGQAQVPGVAGT
                     WKDLTDSVNSMAGNLTAQVRNIAEVTTAVARGDLSRKITVDVKGEILELKNTINTMVD
                     QLNAFAAEVTRVAREVGTEGKLGGQAQVPGVAGTWKDLTDNVNVMAANLTEQVRGIVK
                     VVTAVADGDLKQNLTVQAKGEVAALADTINNMTSTLAIFADQVTTVAREVGVEGRLGG
                     QANVPGAAGTWKDLTGNVNLLAANLTTQVRAIAEVATAVTKGDLTRSIQVEARGEVAE
                     LKDNINTMIGNLRLTTDRNTEQDWLKTNMARFTGMLQGQRELTTVGRTLLSELVPLVS
                     AHQGVIYRMVEGDNMRHLQLLAGYGNVAGGYPESLALGEGFLGQCALDMRRILVTEVP
                     PDTVPIGNALFQALPRSMVVFPVVFEGQLKAVIGLASLTDFAPSHVAFLEQLTASIGI
                     VLNSIEATMQTEALLTQSQQLATELQTQQKELQGKNEQLAQKAQQLAEQNAEVERKNQ
                     EIEQARRALEEKAAELALTSKYKSEFLANMSHELRTPLNSILILGQQLSDNADGNLSP
                     KQVEFARTIHGAGTDLLNLISDILDLSKIESGTVTVEAEETSFASLLATMERTFRHEA
                     ENRRLSFDITTDPGLAKSIVTDSKRLMQVLKNLLSNAFKFTEVGGIRVRVGPAAGGWS
                     TDHPVLRSESSVIAFEVSDTGIGIPQEKQKLIFEAFQQADASTSRKYGGTGLGLAISR
                     ELAFLLGGEIQLRSVPGTGSTFTLYVPLRYAGSSVPTRMDDKNIAPSRPSPFPAIRPP
                     ERAPVQVPDDRNNIEGGDNVLLIVEDDPHYAGLLVNLAREKGFKVLVAMRGTDALSLA
                     REYRPTAVSLDVFLPDMLGWTVLSQLKKDPATRHIPVQMVTLDEDRQHGLARGAFSFV
                     TKPTTTEGLEDALMRIRDYSAPRRKRLLVVEDNAAERMGITELLGHDDIDITSADTGA
                     EALTLLKAMPYDCVVLDLRLPDMSGFEVLERIRDHGTLSDVPVVVFTGKELSAEEDAR
                     LRTLARSVVVKGVESPERLLDETSLFLHRVFADLPAEKQQMLKRLHDSDEDLQGKSVL
                     VVDDDTRNIFALSSVLERRGMTVLTANTGREAISTIESSEDVAIVLMDIMMPEMDGYQ
                     TMQVIRKNPELRRLPIIALTAKAMKGDREKCLEAGASDYLAKPVNTEQLLSVLRVWLH
                     R"
BASE COUNT          735 a         1242 c         1208 g          543 t
ORIGIN      
        1 ctgaaagaga ccctcaacac gatggtcgac cagctcaacc gcttcgccgg cgaggtgacg
       61 cgcgtcgcgc gcgaggtggg gaccgaaggg cggctcggcg gtcaggcgca ggtgccgggc
      121 gtcgccggca cctggaaaga cctcaccgac agcgtgaact cgatggccgg caacctgacc
      181 gctcaggtgc gcaacatcgc cgaggtgacc accgcagtgg cgcgcggcga cctgtcgcgc
      241 aagatcaccg tcgacgtgaa gggcgaaatc ctcgagctga aaaacaccat caacacgatg
      301 gtcgaccagc tcaacgcatt cgccgccgag gtgacgcgcg tggcgcgcga ggtcggcacc
      361 gaaggaaaac tcggcggcca ggctcaggtg cccggtgttg cgggaacgtg gaaggacctc
      421 accgacaacg tcaacgtgat ggcggccaac ctcaccgagc aggtgcgcgg catcgtcaag
      481 gtggtgaccg cggtggccga cggcgacctg aagcagaacc tgacggtgca ggccaagggc
      541 gaagtggcgg cgctggccga caccatcaac aacatgacct cgacactggc gatcttcgcc
      601 gaccaggtga ccacggtggc gcgcgaggtc ggcgtcgagg gccgccttgg cggccaggcc
      661 aacgtgccgg gcgccgccgg cacgtggaag gatctcaccg gcaacgtgaa cctgctcgcg
      721 gcaaacctga ccacgcaggt gcgcgccatc gcggaagtgg caaccgcggt gaccaagggc
      781 gacctcacgc gctcgatcca ggtcgaggcg cgcggcgaag tggccgagct gaaagacaac
      841 atcaacacca tgatcggcaa cctgcgcctg accaccgatc gcaacaccga gcaggactgg
      901 ctgaagacca acatggcgcg cttcaccggc atgctgcagg gccagcgcga actgaccacg
      961 gtgggccgca cactgctgtc ggaactggtg ccgctggtgt cggcgcacca gggcgtgatc
     1021 taccgcatgg tggaaggcga caacatgcgc cacctgcagc tgctggcggg ttatggcaac
     1081 gtggccggcg gctacccgga gagcctggcg ctgggcgaag gcttccttgg ccagtgcgcg
     1141 ctcgacatgc gccgcatcct ggtcaccgag gtgccgcccg ataccgtgcc gatcggcaac
     1201 gcactgttcc aggcgctgcc gcgcagcatg gtggtgtttc cggtcgtgtt cgaaggccag
     1261 ctgaaagcgg tcatcggcct ggcctcgctg accgatttcg cgccgtcgca cgtcgccttc
     1321 ctcgaacagc tcaccgcgtc gatcggcatc gtgctcaact cgatcgaagc gaccatgcag
     1381 accgaggcgc tgctcacgca gtcgcagcag ctggccaccg aactgcagac ccagcagaag
     1441 gaactgcagg gcaagaacga gcagctggcg cagaaagccc agcagctggc ggagcagaac
     1501 gccgaggtgg agcgcaagaa ccaggaaatc gagcaggcgc gccgcgcgtt ggaagaaaaa
     1561 gcggccgagc tcgcgctcac ctcgaagtac aagtcggagt tcctggccaa catgtcgcac
     1621 gagctgcgca cgccgctcaa ctcgatcctg atcctgggcc agcagctgtc cgacaacgcc
     1681 gacggcaacc tttcgcccaa gcaggtggaa ttcgcgcgca ccatccacgg cgccggcacc
     1741 gacctgctaa acctgatctc cgacattctc gacctgtcga agatcgaatc gggcacggtc
     1801 acggtggaag cggaggaaac cagcttcgct tcgctccttg ccaccatgga acgcaccttc
     1861 cgccacgaag ccgaaaaccg ccggctgtcg ttcgacatca ccaccgatcc gggcctggcc
     1921 aagagcatcg tcaccgactc caagcgcctg atgcaggtgc tgaagaacct gctgtcgaac
     1981 gcgttcaagt tcaccgaggt gggcggcatc cgcgtgcgcg tggggccggc ggccggcggc
     2041 tggagcaccg atcacccggt gctgcgcagc gagagctcgg tcatcgcctt cgaggtatcg
     2101 gacaccggca tcggcattcc gcaggaaaaa cagaaactga tcttcgaggc gttccagcag
     2161 gccgacgcgt ccacctcgcg caagtacggc ggcaccggcc tgggcctcgc catctcgcgc
     2221 gaactcgcct tcctgctcgg cggcgaaatc cagctgcgct cggtgccggg caccggcagc
     2281 accttcacgc tctacgttcc gctgcgctac gccggttcgt ccgtgcccac gcgcatggac
     2341 gacaagaaca ttgcgccatc gcggcccagc ccgttcccgg ccatccgtcc gccggagcgc
     2401 gcgccggtgc aggtgccgga cgaccgcaac aacatcgagg gcggcgacaa cgtgctgctg
     2461 atcgtggaag acgatccgca ctacgctggc ctgctggtga atctggctcg cgagaaaggc
     2521 ttcaaggtcc tggtggccat gcgcggcacc gacgcgctgt cgctggcgcg cgagtatcga
     2581 ccgaccgccg tgtcgctcga cgtgttcctg cccgacatgc ttggctggac cgtgctgtcg
     2641 cagctgaaga aggacccggc cacgcggcat atcccggtgc agatggtcac gctggacgag
     2701 gaccgccagc atggcctggc ccgcggcgca ttctcctttg tcaccaagcc gaccaccacc
     2761 gaaggtctcg aagatgcgct gatgcgcatc cgtgactact cggcaccgcg gcgcaagcgc
     2821 ctgctggtcg tggaggacaa cgccgccgaa cgcatgggca tcaccgagct gctcggccac
     2881 gacgacatcg atatcaccag cgcggacacc ggcgccgagg cgctgaccct gctcaaggcc
     2941 atgccctacg actgcgtggt gctggatctg cgcctgcccg acatgtccgg cttcgaagtg
     3001 ctggaacgca tccgcgacca cggcacgctg tccgatgtgc cggtggtggt gttcaccggc
     3061 aaggaactgt cggccgaaga ggatgcacgc ctgcgcacgc tggcgcgcag cgtggtggtc
     3121 aagggcgtgg agtcacccga gcgcctgctc gatgaaacct cgctgttcct gcaccgcgtg
     3181 ttcgccgacc tgccggcgga gaagcagcag atgctcaagc ggctgcacga ttcggatgaa
     3241 gacctgcagg gcaagagtgt gctggtggtg gacgacgaca cgcgcaacat cttcgcgctg
     3301 tcgagcgtgc tggaacgccg cggcatgacc gtgctgaccg ccaacaccgg gcgcgaagcg
     3361 atctccacca tcgaatcgtc ggaagacgtc gccatcgtgc tgatggacat catgatgccg
     3421 gagatggacg gctaccagac catgcaggtg atccgcaaga atcccgagct gcgccgcctg
     3481 ccgatcattg ccctcacggc gaaagccatg aagggcgacc gtgaaaaatg cctggaggcc
     3541 ggcgcctccg attacctggc caagcccgtg aataccgaac agctgctgtc ggtactgcgc
     3601 gtctggctgc accgctgaca cgatgcgcat gaaagacccg gtcaacatcc tgctggtgga
     3661 cgatcagccc ggcaagctgc tcacttacga gaccatcctt tcggaactcg gagagaatct
     3721 ggtcaagg
//
LOCUS       DASZZC010000402         5658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_998881,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000402 DASZZC010000000
VERSION     DASZZC010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998881"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..906
                     /locus_tag="VHE37_08540"
     CDS             118..906
                     /locus_tag="VHE37_08540"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HVT35618.1"
                     /translation="MAQFTLPKNSTLDKKAGKRYPAPAGARKVRSFKIYRYDPDSGAN
                     PREDVYEVDMAACGPMVLDALIKIKNEVDPTLTFRRSCREGICGSCAMNIDGTNTLAC
                     IKSCEEVTSDEVRILPLPHMPVVKDLVPDMTHFYAQLASVEPWLKTDTPAPTRERLQS
                     EEDRARLDGLYECILCACCTTSCPSYWWNGDRYLGPAVLLQAYRWLVDSRDEATGARL
                     DELEDPFKLYRCHTIMNCAKTCPKGLNPAKAIAETKKMLVERRG"
     gene            1029..1457
                     /locus_tag="VHE37_08545"
     CDS             1029..1457
                     /locus_tag="VHE37_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HVT35619.1"
                     /translation="MTEKKPVIYLTRRETFSAAHRLWAEHLSDADNRALFGPCAYDHG
                     HGHNYVLEVTLRGPVDPATGILVNLKEIRDVMHELIIGPVDHRHLNHDSPLTRGMNPT
                     AENLVVLFWNVLHGHYGRLLHEVRLRETEKNWVTYRGERQ"
     gene            1454..2449
                     /locus_tag="VHE37_08550"
     CDS             1454..2449
                     /locus_tag="VHE37_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT35620.1"
                     /translation="MRRLAIAALALLLGACNHDSNDGIYQVDGEPALDVPRADLEAGL
                     KCLPYTHPDKPPVLLVHGTFTNANEQYDWTYIPLLDQRGYDVCAVTYPDRGLGDQQVS
                     AEYVVYALRKMHADSGRRVAMIGHSQGATMPRWALKWWPSARNAVQTFVMQAGPNHGT
                     TVAASTIPVVGEPAAFWQFSPASNFVAALNSGDETPGDIAYTNLYTTFDELVEPSAPI
                     PTAALDWQQGNPKVANILLQDVCAGRVVDHVTIGTTDALSFALALDAVSHGDVANVER
                     AGGSAGLCGLLQVVPDQQLESSAVLDMLSVLPTESTAPPDPQLVTEEPPLKPYAQ"
     gene            2698..3948
                     /locus_tag="VHE37_08555"
     CDS             2698..3948
                     /locus_tag="VHE37_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT35621.1"
                     /translation="MDQNESMTTEHVGMLIVGGGQAGGEIAAELRRQKYEGRVLIVGD
                     EKFLPYKRPPLSKAYFAGSVQEENLYVMQQANLDKAQIEYRGGVAAARIDRAGKALHL
                     ADGRAYSYDKLALATGGRARGLSVPGADKSNVFALRTIADVQKIRALCAPGRRMCIVG
                     GGFIGLEVAAVAIKLGLKVTLLEGLPRVLARVTVPQVSEFFERMHREAGVDLRTSAQL
                     KSFEGGAAVTHVALGDGTQIETDVVVVGIGILPNVELAQAAGIACDNGIVVDSATRTN
                     DPDIVAAGDCTNHPSDFLNRRVRLESVQNAMEQARAAAHAMLGREVNYQMVPWFWSDQ
                     YDLKLQMVGISTGFDQVIFRGDLKTQRNFAAFYLGDGKIIAADTVSRPQEFMLAKKLV
                     AAKAKLDPAKLADESVPLKDLVPA"
     gene            3949..4269
                     /locus_tag="VHE37_08560"
     CDS             3949..4269
                     /locus_tag="VHE37_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT35622.1"
                     /translation="MARLVFIQHDGATQEVQANAGQSVMEAATGAMVRGIVGECGGGC
                     SCATCHVYVDEPWFSQLPPADDMEKGMLEGAVEPGPRSRLSCQIKVSDAIDGLVARIP
                     KGQV"
     gene            4388..5635
                     /locus_tag="VHE37_08565"
     CDS             4388..5635
                     /locus_tag="VHE37_08565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT35623.1"
                     /translation="MLNTRLIKVARDLASVSSIDHAAECDPRDAGMTPKGVERIWNAV
                     ETLYKTGTHPAITLVVRRHGKVVLKRAIGHLRGNGPGEDFEEKVPLSPDAPICLFSAS
                     KAISALLIHKLVEDGRLRLEDRVADYIPEFAAHGKGAVTIRQLMAHRAGIPTIPMKHP
                     DPAMLQHWDALVHMLCLAPPSDPQFEQQAYHALTSGFIIGELVRRASQRELREVMDDW
                     ISRPLQLKYLNFGLPRELRAASPHNYNTGPKPFWPVTTFSKRILGVSFERATEASNGD
                     GFMSAVVPAGNIYASADDACRVFEMLLRGGELDGARVLKAETVAEAIRPVGKIQYDRM
                     LMVPIRFSAGFILGENPFGLYGPTCQQAFGHLGFLSVMCWADPQRDISVALLNTGKSI
                     APSGVLALVRVLGAIARSCPKLR"
BASE COUNT         1038 a         1867 c         1860 g          893 t
ORIGIN      
        1 ccggcgacaa ggacgtgaag atcgatttcc gcccggtgca catgaatccg ctcgacgacg
       61 aagtcgcgca gataccgccg accgcccgca agtactgatc gacaggcaga acaccacatg
      121 gcccagttca cgctcccgaa aaattccacc ctcgacaaga aggcaggcaa gcgctacccc
      181 gcgccggccg gcgccaggaa ggtgcgcagc ttcaagatct accgctacga cccggacagc
      241 ggcgccaatc cgcgcgagga tgtgtacgag gtcgacatgg ccgcctgcgg cccgatggtt
      301 ctcgacgcgc tgatcaagat caagaacgaa gtcgatccca cgctcacctt ccgccgctcg
      361 tgccgcgaag gcatctgcgg ctcgtgcgcg atgaacatcg acggcaccaa cacgctggcg
      421 tgcatcaagt cgtgcgagga agtgacgtcg gacgaggtgc gcatcctgcc gctgccgcac
      481 atgccggtgg tgaaagacct cgtgccggac atgacgcatt tctacgcgca gctggcctcg
      541 gtcgaaccct ggctcaagac cgatacgccc gcgcccacgc gcgagcgcct gcagagcgag
      601 gaagaccgcg ccaggctcga tggcctctac gagtgcatcc tgtgcgcctg ctgcaccacc
      661 agctgcccga gctactggtg gaacggcgac cgctacctcg gccccgccgt gctgttgcag
      721 gcctaccgct ggctggtcga cagccgcgac gaggcgacgg gggcacgtct cgacgagctg
      781 gaagacccgt tcaagctgta ccgctgccac accatcatga actgcgcgaa gacctgccct
      841 aagggcttga acccggcgaa ggcgattgcg gaaaccaaga agatgctggt cgagcgtcgc
      901 gggtgaaaag ctgctgtact cggcatcttg tgcgccggcg gtcctcggaa tcctcacata
      961 cttctctgta tgctccggtt cctgcgctcc gtcctcccgc aaactgcctt cgctcgcgac
     1021 gctcgtccat gactgaaaag aagcccgtca tctacctcac gcgccgcgag accttctcgg
     1081 ccgcgcaccg gctgtgggcg gaacatttgt ccgatgccga caaccgcgcg ctgttcgggc
     1141 catgcgcgta cgaccatggc cacgggcaca actacgtgct cgaggtgacg ctgcgcgggc
     1201 cggtcgatcc cgccaccggc atcctggtga acctcaaaga gatccgcgac gtgatgcacg
     1261 agctgatcat cggtccggtg gatcatcgcc atctcaacca tgactcgccg ctgacccgcg
     1321 gcatgaatcc caccgcggaa aacctggtgg tgctgttctg gaacgtgctg cacgggcact
     1381 acggcaggct gctgcacgag gtgcgcctgc gcgagaccga gaagaactgg gtgacatacc
     1441 ggggagagag gcaatgagaa ggctggccat cgccgcgctg gcgctgctgc tgggtgcgtg
     1501 caaccacgac agcaatgacg gcatctacca ggtggacggc gaaccggcgc tcgacgtgcc
     1561 gcgcgccgat ctcgaagccg gcctcaagtg cctgccctac acgcaccccg acaagccgcc
     1621 ggtgctgctg gtgcacggca ccttcaccaa cgccaacgag cagtacgact ggacctacat
     1681 cccgctgctc gaccagcgcg gctacgacgt gtgcgcggtc acctatcccg accgcgggct
     1741 gggcgaccag caggtgtcgg cggaatacgt ggtctacgcg ctgcgcaaga tgcacgccga
     1801 cagcgggcgc cgcgtggcga tgatcggcca cagccagggc gccaccatgc cgcgctgggc
     1861 gctgaagtgg tggccgagcg cgcgcaacgc ggtgcagacc ttcgtcatgc aggccgggcc
     1921 caaccacggc accacggtgg cggcgtcgac catacccgtg gtcggcgagc cggcggcgtt
     1981 ctggcagttc tcgcctgctt ctaatttcgt agcagcgctg aacagcggcg acgagacgcc
     2041 gggcgacatc gcctacacca acctctacac caccttcgac gagctggtgg agcccagcgc
     2101 gccgattccc accgctgcgc tcgactggca gcagggcaat ccgaaagtcg ccaacatcct
     2161 gctgcaggac gtgtgcgccg ggcgcgtggt cgaccatgtg accatcggca ccaccgacgc
     2221 gctgtcgttc gcgctggcgc tggacgcggt gtcgcacggc gacgtggcca atgtcgagcg
     2281 cgccggcggc tcggccggcc tgtgcgggct gctgcaggtg gtgccggacc agcagctgga
     2341 atccagcgcg gtgctcgaca tgctgtcggt gctgccgacc gaatcgaccg cgccgcccga
     2401 tccgcaactg gtcaccgagg agccgccgct caagccttac gcgcagtgaa acgcgcgcga
     2461 gattgcgcgc gacattgcgc tttttcaggc gaaaataagg ttgaccgcac tgctgattgt
     2521 cgtgttataa aatatttgat gttttaataa acataagata tttaataaca tcgaaaatga
     2581 gggtgcggtg agaggaattt caggccgcgc aacggcgccc gggctacttc aaatggtgca
     2641 agacccagca cgcacgctct gctgtcatgc gcagcaggga gccgcggcgc ggcagagatg
     2701 gaccagaacg aatcgatgac gactgaacac gtgggcatgc tgatcgtcgg cggcggacag
     2761 gccggcggcg agatcgcggc ggagctgcgg cgccagaaat acgagggccg cgtgctgatc
     2821 gtgggcgacg agaaattcct gccgtacaag cgcccgccgc tgtcgaaggc ctatttcgcc
     2881 ggcagcgtgc aggaagaaaa tctctacgtg atgcagcagg ccaacctgga caaggcgcag
     2941 atcgaatacc gcggcggcgt ggcggctgcg cgcatcgacc gcgccggcaa ggcgctgcac
     3001 ctggccgacg gccgcgccta cagctacgac aagctggcgc tggccaccgg cggacgcgcg
     3061 cgcgggctgt cggtgccggg cgcggacaag tccaacgtat tcgcactgcg caccattgcc
     3121 gacgtgcaga agatccgcgc gctgtgcgcg ccgggcaggc gcatgtgcat tgtcggcggc
     3181 ggcttcatcg ggctggaagt ggcggcggtg gcaatcaagc tcgggctcaa ggtcacgctg
     3241 ctcgaaggcc tgccgcgcgt gctggcgcgc gtcaccgtgc cgcaggtgtc ggagttcttc
     3301 gagcgcatgc accgcgaggc cggtgtcgac ctgcgcacca gcgcgcagct gaaatctttt
     3361 gaaggcggtg ctgccgttac ccacgtcgcg cttggcgacg gcacgcagat cgagacggat
     3421 gtcgtggtgg ttggcatcgg catcctgccc aatgtcgaac tggcccaggc cgccggcatc
     3481 gcctgcgaca atggcatcgt ggtcgacagc gccacgcgca ccaatgaccc ggacatcgtc
     3541 gccgccggcg actgcaccaa ccacccgagc gatttcctca accgccgcgt gcggctggaa
     3601 tcggtgcaga acgcgatgga gcaggcgcgc gcggccgcgc acgccatgct cgggcgcgag
     3661 gtgaactacc agatggtgcc gtggttctgg agcgaccagt acgacctcaa gctgcagatg
     3721 gtcggcattt ccaccggctt cgaccaggtg atcttccgcg gcgatctcaa gacgcagcgg
     3781 aatttcgccg cgttttacct gggcgacggc aagatcatcg ccgccgacac cgtcagccgg
     3841 ccgcaggaat tcatgctggc gaagaaactg gtcgcggcga aggcgaagct tgacccggcg
     3901 aagctggccg acgagtcggt cccattgaaa gacctggtgc cggcataaat ggccaggctc
     3961 gtcttcatcc agcacgacgg cgccacgcag gaagtgcagg ccaatgccgg ccagagcgtg
     4021 atggaagccg ccaccggcgc catggtgcgc ggcatcgtcg gcgagtgcgg cggcggctgc
     4081 tcgtgcgcca cctgccatgt ctatgtcgac gagccgtggt tctcgcagtt gccgccggcc
     4141 gacgacatgg agaagggcat gctggaaggc gcggtggaac cgggtccgcg ctcgcggctg
     4201 tcgtgccaga tcaaggtgag cgatgccatc gatgggctgg tggcgcggat tcccaaggga
     4261 caggtctgac caatccaatc cgtcattccc gcgaaggtgg gaatccagtc ttgctttttt
     4321 ttctccaccc gccgaaaact ggatgcccgc cttcgcgggc atgacaggaa atagtcagag
     4381 tcgaaatatg ctcaacaccc gcctgatcaa agtcgcgcgc gacctcgcca gcgtcagcag
     4441 catcgaccat gcggcggagt gcgatccgcg cgacgccggc atgacgccca agggcgtcga
     4501 gcgcatctgg aacgcggtgg agacgctgta caagaccggc acgcatccgg ccatcacgct
     4561 ggtagtgcgc cgccacggca aagtcgtgct caagcgcgcc atcggccacc tgcgcggcaa
     4621 cgggccgggc gaggatttcg aggagaaggt gccgctgtcg ccggatgcgc cgatctgcct
     4681 gttctcggca tcgaaggcga tcagcgcgct gctgatccac aagctggtgg aagacggcag
     4741 gctgcgcctg gaagaccgcg tggcggatta catccccgag ttcgccgcgc acggcaaggg
     4801 cgccgtcacc atccgccagc tgatggcgca ccgtgccggc atcccgacca tcccgatgaa
     4861 gcacccggac ccggcaatgc tgcagcactg ggatgcgctg gtgcacatgc tgtgcctggc
     4921 gccgccgtcc gatccgcagt tcgagcagca ggcgtaccac gcgctgacca gcggcttcat
     4981 catcggcgaa ctggtgcggc gcgccagcca gcgcgaactg cgcgaggtga tggacgactg
     5041 gatctcgcgt ccgctgcagc tcaagtacct gaacttcggc ttgccgcgtg agctgcgcgc
     5101 ggcgtcaccg cacaactaca acaccgggcc caagccattc tggccggtga ccacgttttc
     5161 caagcgcatc ctcggcgttt cgttcgagcg cgccaccgag gcgtcgaacg gcgacggctt
     5221 tatgtcggcg gtggtgccgg cgggcaacat ctatgcctcg gccgatgacg cctgccgcgt
     5281 gttcgagatg ttgctgcgcg gcggcgagct cgacggcgcg cgcgtgctga aggccgagac
     5341 ggtggcggag gcgatccgcc cggtcggcaa gatccagtac gaccgcatgc tgatggtgcc
     5401 gatccgcttt tccgccggct tcatcctcgg cgagaacccg ttcggcctgt acggccccac
     5461 ctgccagcag gcgttcggcc atctcggatt cctttcggtg atgtgctggg ccgatccgca
     5521 acgcgacata tcggtggcgc tgctcaacac cggcaagtcg atcgcgcctt cgggcgtgct
     5581 cgcgctggtg cgcgtgctcg gggcgattgc gcgcagctgc ccgaagctgc gctagcggcg
     5641 gtcagcccgg cggctcat
//
LOCUS       DASZZC010000403         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_673771,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000403 DASZZC010000000
VERSION     DASZZC010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673771"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VHE37_08570"
     CDS             <1..1178
                     /locus_tag="VHE37_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35624.1"
                     /translation="SASTGTITVSVADGGEDGAAPATLAWPITVVSQAHLCALPDVNG
                     NGSPDQALLVKTPAGVSAYVTEGAGATLIRTVTFASAYTAAGLACIDDLNGNGTPELA
                     ALGKSTSTGAIQVQIRDAGSGALIRNIPFLGPGYQPVAVASATSGTTPLLAVLGKDAT
                     AGTVRVQLKNALDGSQVKTINFFSPTSSAQALALATGLQGANSVQVAVLGKDAATGIV
                     HVQLRDASSGALINTINFLTPAFSAERLAIIPAPAKVGGAAIAVLGRDPVAGVIRVQL
                     RDAFSDAVDRTLTFFGAHYEPEDLAIVDGAATPQVAVLARNLDTGVVKVQMLDSAGGT
                     ALGTVTFPGPSFVPMQLVRVTDGSGAQGLAVLSDGATLRVQTRSASDGSELGSATYP"
     gene            1378..>2384
                     /locus_tag="VHE37_08575"
     CDS             1378..>2384
                     /locus_tag="VHE37_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT35625.1"
                     /translation="MTRASMEGIIEAVYREHSRAVLATLIRLLGDFDLAEEALHEAFR
                     AALEQWPGGGVPENPRAWLVSAGRFKAIDQLRRQARFDPLDEAQHDVGVEDPELADAD
                     SVEDDRLRLIFTCCHPALSPEAQVALTLREVCGLTTEEIAAAFLTPAPTLAQRIVRAK
                     GKIRDARIPYEVPATAELHQRLDSVLQVIYLVFNEGYSASSGAELTRHDLSGEAIRLG
                     RLLAQLLPDAEVFGLLALMLLHDSRRAARADADGELILLEEQDRALWNRAQIDEGVAL
                     VERALVMRRPGPYGVQAAIAALHAQAPSAAATDWPQVVALYDLLLRMAPSPVVELNRA
                     VA"
BASE COUNT          380 a          840 c          826 g          338 t
ORIGIN      
        1 cttccgccag caccggcacc atcacggtca gcgtcgccga tggcggcgag gacggcgccg
       61 cgccggccac gctcgcctgg ccgatcaccg tggtgtcgca ggcgcatctg tgcgcactgc
      121 ccgacgtcaa cggcaacggc agccccgacc aggcgctgct ggtgaagacg ccagcgggcg
      181 tgtccgccta cgtcaccgag ggcgccggcg ccacgctgat ccgcaccgtg accttcgcct
      241 ctgcttacac tgccgcaggc ctcgcctgca tcgatgacct caacggcaac ggcacgccgg
      301 aactggcggc gctgggcaag agcaccagca ccggcgcgat acaggtgcag atccgcgacg
      361 ccggcagcgg cgcgctgatc cgcaacattc ccttcctcgg acccggctac cagccggtgg
      421 cggtcgcgtc cgccaccagc ggcaccacgc cgctgctcgc agtgctgggc aaggatgcga
      481 ccgccggcac cgtgcgcgtg cagctgaaga acgcgctcga cggctcgcag gtcaagacga
      541 tcaacttctt ctcgcccacg tccagcgcgc aggcgctcgc gctggcaacc ggcctgcagg
      601 gcgccaacag cgtgcaggtc gcggtactgg gcaaggatgc ggcgaccggc atcgtgcacg
      661 tgcagctgcg cgatgcctcc agcggcgcgc tgatcaacac catcaacttc ctcacgccgg
      721 cgttcagcgc ggagcggctg gccatcatcc cggcgccggc caaggtcggc ggcgcggcga
      781 tcgcggtgct gggacgcgac ccggtggcag gcgtcatccg cgtgcagctg cgcgatgcct
      841 tcagcgacgc ggtggaccgg acgctcacct tcttcggcgc gcattacgag ccggaagatc
      901 tggccatcgt cgacggcgcg gcaacgccgc aggtggcggt gctggcgcgc aatctcgaca
      961 ccggcgtggt caaggtgcag atgctcgaca gtgccggtgg cacggccctg ggcacggtca
     1021 cctttcccgg cccgtcgttc gtgccgatgc aactcgtgcg cgtcaccgac ggcagcggcg
     1081 cgcaggggct ggccgtgctc agcgacggcg ccacgctgcg cgtgcagacg cgttccgcca
     1141 gcgacggcag cgaactcggc agcgcaacct atccgtaaac gccgcgcgac ccgctgaaaa
     1201 aaagaaagcc agcccggagg ctggcttgaa gaccgctcga tcctggggat ggatgcgtga
     1261 gcggtgggga gattcaggtt gcagtttagg gccgagttgt ccccgctcaa tcgacctgat
     1321 gcacccaatg cctgtggata agcaaacgga cacgcgactc acgctaacct tgcgcccatg
     1381 acgcgcgcat ccatggaagg aatcatcgaa gcggtctacc gcgagcattc gcgcgcggtt
     1441 ctggccacgc tgatccgcct gctcggcgat ttcgacctgg ccgaggaagc gctgcacgag
     1501 gcgttccgcg ccgcgctgga acaatggccg ggcggcggcg tgccggaaaa tccgcgcgcc
     1561 tggctggtct cggccgggcg cttcaaggcc atcgaccagc tgcgccggca ggcgcgtttc
     1621 gatccgctgg acgaggccca gcacgacgtc ggcgtggaag acccggaact ggccgacgcc
     1681 gacagcgtcg aggacgaccg cctgcggctg atcttcacct gctgccaccc cgcgctgtcg
     1741 cccgaggcac aggtggcact gaccctgcgc gaggtgtgcg gactgacgac ggaagagatc
     1801 gctgcggcct tcctcacgcc ggcgccgacg ctggcgcaac gcatcgtgcg cgccaagggc
     1861 aagatccgcg acgcgcgcat tccgtacgaa gtgccggcca cggccgagtt gcatcagcgg
     1921 ctcgactccg tgctgcaggt gatctacctg gtgttcaacg agggttactc ggccagctcg
     1981 ggcgccgagc tgacgcggca cgacctgtcc ggcgaagcca tccgcctggg ccgcctgctg
     2041 gcgcagctgc tgcccgacgc ggaggtgttc ggcctgctgg cgctgatgct gctgcacgac
     2101 tcgcggcgcg cagcgcgggc tgatgccgac ggcgagctga tcctgctcga ggaacaggac
     2161 cgcgccctgt ggaaccgtgc gcagatcgat gaaggcgttg cgctggtgga acgcgcgctg
     2221 gtcatgcgcc ggccgggacc ttacggagtg caggcggcca tcgccgcgct gcatgcacag
     2281 gcaccaagcg ccgccgccac cgactggccg caggtggtgg cgctgtacga cctgctgctg
     2341 cgcatggcgc cgtcgccggt ggtcgagctc aaccgcgcgg tggc
//
LOCUS       DASZZC010000404         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_354495,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000404 DASZZC010000000
VERSION     DASZZC010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354495"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..624
                     /locus_tag="VHE37_08580"
     CDS             37..624
                     /locus_tag="VHE37_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVT35626.1"
                     /translation="MGMWIVLGLIVLAVFFLIGTYNGLVAARNGYKNAFAQIDVQLQR
                     RNDLIPNLVETAKGYMKHERETLEAVIAARNSAAAGLKQAQLNPGDAAAIKQLGAAEG
                     ALAGAMTRFMALAEAYPDLKANQNMMQLSEELSSTENRVAFARQAFNDAVLGYNNSRE
                     VFPNSVIAGMFNFAPAQMWEIEKPEMRAAPKVSFT"
     gene            704..2617
                     /locus_tag="VHE37_08585"
     CDS             704..2617
                     /locus_tag="VHE37_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVT35627.1"
                     /translation="MNFFQHQAQARSQTKWLVFMFALAVGAIVAAIDFVLWLALSASD
                     NGQHHGHYAHAAQQDYSGALVAASFAVIALIAIASLVRSATLRSGGGAVARGLGAVLV
                     PPEARDPRYRRLRNVVEELAIASGTPVPEIYVMEGETAINAFAAGWSPADAAITVTRG
                     ALDKLDRDELQGVIAHEFSHVTNGDMRLSIRLMGVLFGILVLSVIAREIFLNTRTGGS
                     RKNDGAAAIIAIALAVWIIGSIGQFFGRMIKATISRQRELLADACAVQFTRQNQGIAG
                     ALKKIAGLEAGSKLHVGRGEEVSHMLFCDGGFSSLFATHPPLLERIRILDPAFKPDQI
                     GAIAARVNDPGYEPADTSAVAAGFAGAETLAVTRAGVTAQVGNPSPDAYAAAAALHAA
                     LPGELRAAAGDAASAWPLIFALLLDADAGVRGRQLQLIEKSFGASTRAAAQALRDAAA
                     ALPPEQRLPLVSLTFPALKTRSREELRPLLEAVSQLIRADGVVTLQEFCLGHLLRTQV
                     ADAINPTRAKAIGRRKLGDARSDALTLLAALAHCGTPDVASARRAFIAGAGYLYPREP
                     ADYQPPADPVAALEQVLPALDALDAPGKELLIGALVETLAFDNRITVSEAELLRVICA
                     ALHCPLPPLLAAA"
     gene            2614..3288
                     /gene="radC"
                     /locus_tag="VHE37_08590"
     CDS             2614..3288
                     /gene="radC"
                     /locus_tag="VHE37_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HVT35628.1"
                     /translation="MTIKDWPEGERPREKLLARGAAALTEAELLAIFLRTGVTGMSAV
                     DLARDLLARFGSLRALLKAPLDEFCAAKGLGEAKYAQLQAVLEMSRRHLAEELASGDA
                     LSGPQATRTYLTARLRGLDHEVFGALFLDSQHRVLGFDELAAGTIDGASVYPREVVKR
                     ALRRNAAAVIFAHNHPSGVAEPSAADRALTERLKSALALVDIRVLDHFVVGEGTPVSF
                     AERGWL"
     gene            3394..3630
                     /gene="rpmB"
                     /locus_tag="VHE37_08595"
     CDS             3394..3630
                     /gene="rpmB"
                     /locus_tag="VHE37_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HVT35629.1"
                     /translation="MSKVCQVTGKRPIVGNTVSHANNKRRRRFEPNLHTHRFWLEPEK
                     RFVSLRVSTSGMRMIDKKGIEAIVADIRKRGEKV"
BASE COUNT          603 a         1258 c         1260 g          572 t
ORIGIN      
        1 aagacgcgca atcccaaaaa acctagggga aacgccatgg gcatgtggat cgttctcggc
       61 ctgatcgtgc tggccgtgtt tttcctgatc ggcacctaca acggcctggt cgccgcgcgc
      121 aacggctaca agaacgcctt cgcgcagatc gacgtgcagc tgcagcggcg caacgacctg
      181 atccccaacc tggtcgaaac cgccaagggc tacatgaagc acgaacgcga aacgcttgaa
      241 gcggtgatcg ccgcgcgcaa cagcgcggcc gccgggctca agcaggcaca gctcaatccg
      301 ggcgacgcgg ccgccatcaa gcagctgggc gcggcggaag gcgcgctggc cggcgcgatg
      361 acgcgcttca tggcgctggc cgaggcctat cctgacctca aggccaacca gaacatgatg
      421 cagctgtcgg aggaactgtc gtccaccgag aaccgcgtgg ccttcgcgcg ccaggcgttc
      481 aacgacgcgg tgctgggtta caacaacagc cgcgaggtgt ttcccaacag cgtcatcgcc
      541 gggatgttta atttcgcgcc ggcgcaaatg tgggaaatcg agaagccgga gatgcgcgcg
      601 gcgccgaagg tgagtttcac ctgaggcttc ctgcagcaca gggcagtgcc tgcgattgat
      661 ccacgaatcg cgggcaaggc ccgctcctac aaatccgcgc gccatgaact tcttccagca
      721 ccaggcccag gcccgcagcc agaccaagtg gctggtgttc atgttcgcgc tggcggtcgg
      781 cgccatcgtc gctgccatcg acttcgtgct gtggctggcg ctcagcgctt cggataacgg
      841 ccagcaccac ggccactacg cgcatgcggc gcagcaggat tattcgggcg cgctggtggc
      901 ggcgagtttc gccgtcatcg cgctgatcgc catcgccagc ctggtgcgca gcgccaccct
      961 gcgcagcggc ggcggcgcgg tggcgcgcgg gctgggcgcg gtgctggtgc cgccggaggc
     1021 gcgcgatccg cgctaccggc ggctgcgcaa cgtggtggag gaactcgcca tcgcctcggg
     1081 cacgccagtg ccggaaatct acgtgatgga gggcgagacg gcgatcaacg cgttcgccgc
     1141 cggctggtcg ccggccgatg ccgccatcac ggtgacgcgc ggcgcgctgg acaagctcga
     1201 ccgcgacgag ctgcaaggcg tgatcgcgca cgagttctcg cacgtgacca acggcgacat
     1261 gcgcctgtcg atccggctga tgggcgtgct gttcggcatc ctggtgctgt cggtgatcgc
     1321 gcgcgagatc ttcctcaaca cgcgcaccgg cggttcgcgc aagaacgatg gcgccgcggc
     1381 gatcatcgcc attgccctcg ccgtgtggat catcggcagc atcggccagt ttttcggccg
     1441 catgatcaag gccaccatct cgcgccagcg cgaactgctg gccgatgcct gcgcggtgca
     1501 gttcacccgc cagaaccagg gcatcgccgg cgcgctgaag aaaatcgccg ggctcgaggc
     1561 gggctcgaag ctgcacgtcg gccgcggcga ggaagtcagc cacatgctgt tctgcgatgg
     1621 cgggttctcc tcgctgttcg ccacccatcc gccactgctc gagcgcatcc gcatcctcga
     1681 tcctgcgttc aagcccgacc agatcggggc gatcgccgcg cgcgtgaatg acccgggcta
     1741 tgaaccggcc gataccagcg cggttgccgc cggatttgcc ggcgcggaga cgctggcggt
     1801 aacgcgcgca ggcgtgaccg cgcaggtggg caatccctcg ccggatgcgt atgccgccgc
     1861 cgccgcgctg cacgcggcgc tgcccggaga gttgcgcgcc gcggccggcg acgcggcgag
     1921 cgcctggccg ctgatcttcg cgctgctgct cgatgccgat gccggtgtgc gcggccgcca
     1981 gctgcagctc atcgagaaaa gttttggagc ttccacccgc gcggccgcgc aggccttgcg
     2041 cgacgccgcc gcggccttgc cgccggagca gcggctgccg ctggtgtcgc tgacctttcc
     2101 cgcgctcaag acgcgcagcc gcgaggaatt gaggccactg ctggaggcgg tatcgcagct
     2161 gatccgcgcc gatggcgtgg tcacgctcca ggagttctgc ctgggccacc tgctgcgcac
     2221 gcaggtggcc gatgcgatca accccacgcg cgccaaggcc atcggccggc gcaagcttgg
     2281 cgatgcgcgc agcgacgcgc tgaccctgct tgctgcgctg gcccattgcg gtacgccgga
     2341 cgtcgccagc gcgcggcgtg ccttcatcgc cggcgccggc tatctctatc ctcgcgagcc
     2401 ggcggactac cagccaccgg ccgacccggt ggccgcgctg gagcaggtac tgccggcact
     2461 cgatgcgctg gatgcgccgg gcaaggaact gctgatcggc gcgctggtgg agacgctggc
     2521 gttcgacaac cgcatcacgg tgagcgaggc ggaactgctg cgcgtgatct gcgccgcgct
     2581 gcattgcccg ctgccgccgc tgctggccgc tgcatgacca tcaaggactg gccggaaggc
     2641 gagcgtccgc gcgagaagct gctggcgcgc ggtgcggccg cactcaccga ggcggaactg
     2701 ctggccatct tcctgcgcac cggcgtcacc ggcatgagcg cggtggatct tgcacgcgac
     2761 ctgctggcac gtttcggcag cctgcgtgcg ctgctcaagg cgccgctgga cgagttctgc
     2821 gcggccaagg gactgggcga ggccaagtac gcccagctgc aggcagtgct tgagatgagc
     2881 cgccgtcacc tggcggagga actcgcctcc ggcgatgcgc tgtcgggccc gcaggccaca
     2941 cgcacctacc tgaccgcgcg cctgcgcggg ctggaccacg aggtgttcgg cgcgctgttc
     3001 ctcgacagcc agcaccgcgt gctgggtttc gacgaactgg ccgccggtac catcgacggc
     3061 gccagcgtgt acccgcgcga ggtggtcaag cgtgcgctcc ggcgcaacgc ggcggcggtg
     3121 atcttcgccc acaaccatcc ctcgggcgtg gccgagccgt cggccgcgga ccgcgcactg
     3181 accgagcggc tgaagagcgc gctggcgctg gtcgatatcc gcgtgctcga ccacttcgtg
     3241 gtcggcgagg gcacgccggt atccttcgcc gagcgcggct ggctgtagcc atgcccattc
     3301 ggcttgatcg ggcaggggca aaactgctat aaagcgcgcc cttttggctc cgccgccccg
     3361 cggctgtggc taaattttca ccaggtacag gtcatgtcca aagtctgtca ggtcaccggc
     3421 aagcggccga tcgtgggtaa cacggtcagc cacgccaaca acaagcgccg ccggcgcttc
     3481 gagcccaatc tgcacaccca ccgtttctgg ctggagccgg aaaagcgctt cgtgtcgctg
     3541 cgcgtgtcca ccagcggcat gcgcatgatc gacaagaagg gcatcgaggc gattgttgcc
     3601 gacatccgca agcgcggcga gaaggtgtaa gcatggccaa gaaagaaacc gagaaggtca
     3661 agctggtgtc caccgcggac acgggctact act
//
LOCUS       DASZZC010000405         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_999522,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000405 DASZZC010000000
VERSION     DASZZC010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999522"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VHE37_08600"
     CDS             complement(<1..1551)
                     /locus_tag="VHE37_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HVT35630.1"
                     /translation="MTDRYDAVIVGSGAGGGVAAYVLARGGMRVAVLEKGPWLTADDF
                     SDDELKTGERHFYDQDPLIEPRTFRDSATSGTRQFTGKVLPVSRCVGGGSVHYGAVCF
                     RFRPEDFRALSTYGNLPGADIQDWPLSDAELDAGNPNSIWSIYGQLEKLLGVAGGQLR
                     GDADPGAPVPGAEWRSARYPMPGHVANYGAKLFEDAAKRLGYNPFPTPVCIANGRYTL
                     SDDALLAGSAPLSRPGCSYCGFCSSHGCPQESKGDTRVTALELARRTGRCDILPDCDA
                     VQIAMKDGRADHVVYIDAQGNTQSIAGDRIVLACGVIDSPRLLLLSELDPALVNYDIV
                     GRYLTVHHFPGAIGVFEQRIDFYKGYWSMRCLDDFYFGLPGAAQKKFGYGNVQTIGPS
                     SGYPLTNGGIIATAKFAPWGVNHKRAMQFLFGHMQWFGMIGQDPPVASNRVDLDPDVK
                     DVYGRPVARITYTHHPNDYEVAGQVLPRFLELLTAMGASTKQVFAPLASPSEIPQLLP
                     QDLLQRGPA"
     gene            complement(1548..2441)
                     /locus_tag="VHE37_08605"
     CDS             complement(1548..2441)
                     /locus_tag="VHE37_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35631.1"
                     /translation="MLSACSGSPQDSAQKDTQASAATSCASSTVVSFPIPQNPLFTLD
                     PIGPLSDVTCDWQHYQPAQRFCVKLDDAELTPGGTGPTPGNGSDPACAQSGFAPDDTG
                     LYIVDIDTPILCPGCRRYFIDLSNIPAPDDSSGATAHGHAFYALASFNFSYTIDPYAH
                     SPNTKNFTNDALVTPPGTPLQTVIGATDMYDKAYVTHTTKFAHTGVQGPYYIGPWYVN
                     RDGERIHGVYLDVDVTDAVSLGNPQLNALRYLVGYNSGRLSCPDNLLANYAGADFLYA
                     GCVDQPGTSSIALDPLDDEAR"
     gene            complement(2555..3325)
                     /locus_tag="VHE37_08610"
     CDS             complement(2555..3325)
                     /locus_tag="VHE37_08610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HVT35632.1"
                     /translation="MKLTRRAFVAGAAATAALPLADCTRSQAPEISTAPAGLADAKLA
                     EFAFTGAQRAALTAAVAQLIPASTPGDWSAADAGAVEYIEQLLNGFSADGNPKIYGHG
                     PARPRFAEFEPLSRVKTHGWMGEVQRLRQVYADGLDELNRLARGPLSLLPGDFSALSA
                     PEQIAILESQDLQDTPFFAALFAHTMEGVYGHPVYGGNRAYIAWQTYCYEGDVHGVRF
                     PDGYAPGDDARPWNEFGGYSPDEIAAAPAACSGKGGST"
BASE COUNT          562 a         1103 c         1217 g          571 t
ORIGIN      
        1 cgcgggcccg cgctggagca ggtcctgcgg cagcagctgc ggaatttccg atggcgaggc
       61 cagcggcgcg aacacctgct tggtgctcgc gcccattgcc gtgagcagtt cgagaaagcg
      121 cggcagcacc tggcccgcca cctcgtagtc gttcggatga tgcgtgtagg tgatgcgcgc
      181 gacggggcgg ccgtacacgt ccttcacgtc aggatcgaga tccacgcggt tactcgccac
      241 cggcgggtcc tggccgatca tgccgaacca ctgcatgtgg ccgaacagga actgcatggc
      301 gcgcttgtgg ttgacacccc acggagcaaa tttcgcggtg gcgataatgc cgccgttggt
      361 cagcgggtag ccgctggacg ggccgatggt ctgcacgttg ccgtagccga attttttctg
      421 cgcggcgccg ggcaggccga aatagaaatc gtccaggcag cgcatcgacc agtagccctt
      481 gtagaaatcg atgcgctgtt cgaacacgcc tatggcgccg ggaaagtgat gcacggtgag
      541 atagcggccg acgatgtcgt agttgaccag cgccgggtcg agctccgaca gcagcagcag
      601 gcgcgggctg tcgatcacgc cgcaggccag cacaatgcgg tcgccggcga tgctctgcgt
      661 gttgccctgc gcgtcgatgt aaaccacgtg atcggcgcgg ccgtccttca tggcgatctg
      721 caccgcgtcg cagtcgggca ggatgtcgca gcggccggtg cgccgcgcca gctccagcgc
      781 ggtcacgcgc gtgtcgccct tggattcctg cgggcagccg tggctggaac agaagccgca
      841 gtaggagcag cccggccgcg acagaggcgc gctgccggcg agcagcgcgt cgtcggacag
      901 cgtgtaccgg ccgttggcga tgcacaccgg cgtggggaag gggttgtagc ccagccgctt
      961 tgccgcgtcc tcgaaaagtt tcgcgccata gttggccacg tggccgggca tcgggtagcg
     1021 cgcgctgcgc cactcggcgc cgggcaccgg cgcgccgggg tcggcgtcgc cgcgcaattg
     1081 tccgccggcc acgcccagca gcttttccag ctgcccatag atcgaccaga tggaattggg
     1141 attgccggcg tcgagctcgg catcggacag cggccagtcc tggatgtcgg cgccgggcag
     1201 atttccgtag gtggagagcg cgcggaaatc ctccgggcgg aagcggaagc acaccgcgcc
     1261 gtaatgcacc gagccgccgc cgacgcagcg cgacaccggc agcaccttgc cggtgaactg
     1321 ccgcgtgccg gaggtggccg agtcgcggaa ggtgcgcggc tcgatcagcg gatcctggtc
     1381 gtagaaatgc cgctcgccgg tcttcagctc gtcgtcggaa aaatcatcgg cggtgagcca
     1441 cgggcctttt tccagcaccg ccacgcgcat gccgccgcgc gccagcacgt aggcggcgac
     1501 gccgccaccg gcgccggagc cgacgatgac cgcgtcatag cggtcagtca tcgggcttcg
     1561 tcatcgagtg gatcgagcgc gatgctggac gtgcccggct ggtcgacgca gccggcgtag
     1621 aggaaatcgg cgccggcgta attcgccagc aggttgtcgg ggcaggagag gcggcccgag
     1681 ttgtagccga cgagataacg cagggcattg agctgcgggt tgcccaggct caccgcgtcg
     1741 gtgacgtcca catccagata cacgccgtgg atgcgctcgc cgtcgcggtt cacgtaccag
     1801 ggcccgatgt agtacggccc ctgcacgccg gtgtgcgcaa acttcgtggt gtgcgtgaca
     1861 taggccttgt cgtacatgtc ggtggcgccg atcacggtct gcagcggcgt gcccggcggc
     1921 gtcaccagcg catcgttggt gaagttcttg gtgttggggc tgtgcgcata cggatcgatc
     1981 gtgtagctga agttgaacga ggccagagcg tagaacgcgt ggccgtgcgc ggtggcgccg
     2041 ctgctgtcgt ccggcgcggg aatgttggac aggtcgatga agtagcgccg gcagcccggg
     2101 cacaggatcg gcgtgtcgat gtcgacgatg tacagtccgg tgtcgtccgg cgcaaagccg
     2161 gactgcgcgc aggccgggtc gctgccgttg cccggcgtag gcccggtgcc gccgggcgtg
     2221 agctcggcgt cgtcgagctt cacgcagaag cgctgcgccg gctggtagtg ctgccagtcg
     2281 caggtgacgt cggacagcgg gccgatcggg tcgagcgtga acagcgggtt ctgcggaatg
     2341 ggaaaactta cgacggtgga actcgcgcaa ctcgtcgcgg ccgatgcctg cgtgtctttt
     2401 tgcgcggagt cctgcggcga tccgctgcag gcgctgagca ttccggcgca aacaatcatc
     2461 atcgtcattc ccgcgcaagc gggaatccat cgtgatgaga cgacgctgct gcggccatgg
     2521 attcccgctt gcgcgggcat gacgaaggaa aatttcacgt ggagcctccc ttgccgctgc
     2581 acgccgccgg agccgcggcg atctcgtccg gcgagtagcc gccgaattca ttccacggcc
     2641 gcgcgtcgtc gccgggcgca tagccatccg ggaagcgcac gccatgtaca tcgccctcgt
     2701 agcagtacgt ttgccacgcg atgtaagcgc ggttgccgcc gtacaccggg tgcccgtaca
     2761 cgccttccat ggtgtgcgcg aacagcgcgg cgaagaacgg cgtgtcctgc aggtcctgcg
     2821 attcgaggat ggcaatctgc tcaggcgccg acagtgcgga gaaatcgccg ggcagcagcg
     2881 acagcgggcc gcgcgccagg cggttcaatt cgtcgaggcc atcggcgtag acctgccgca
     2941 ggcgttgcac ctcacccatc cagccatgcg tcttcacgcg cgacagcggt tcgaattcgg
     3001 cgaagcgcgg gcgcgccggc ccgtggccat aaatcttcgg attgccgtcg gcggaaaaac
     3061 cattgagcag ctgctcgatg tattccaccg cgccggcatc ggcagcgctc cagtcgccgg
     3121 gcgtgctggc gggaatcagc tgcgccaccg ccgcagtcag tgccgctcgc tgcgcaccgg
     3181 tgaaggcgaa ctccgcgagc ttcgcatcgg ccaggccggc cggtgccgtg gaaatctccg
     3241 gcgcctgcga gcgtgtgcaa tccgccagcg gcagggcggc ggtggccgcg gcgcctgcaa
     3301 cgaatgcacg tcgcgtgagt ttcacttcgc tggcgccgca atgcccagtg tgccggtggt
     3361 ctccgacttg ggtaccgagt catgcgtgcc gaaggtgccg aacaggcggt cacccatgcc
     3421 catcgacacg ccccaccagt atttctcgtt gcg
//
LOCUS       DASZZC010000406         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_354654,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000406 DASZZC010000000
VERSION     DASZZC010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354654"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHE37_08615"
     CDS             <1..318
                     /locus_tag="VHE37_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35633.1"
                     /translation="IRADEGDLYELFGNLLDNAAKWCRHQVRVVAAHQGNRVHLLVED
                     DGAGFPEDAEALLERGVRADTRVAGQGIGLSAVAELVAAYEGRITLGRSATLGGALVE
                     ITL"
     gene            complement(437..985)
                     /gene="orn"
                     /locus_tag="VHE37_08620"
     CDS             complement(437..985)
                     /gene="orn"
                     /locus_tag="VHE37_08620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HVT35634.1"
                     /translation="MNANASNLIWIDLEMTGLSPEQHRIIEIATLVTDQDLNVLAEGP
                     VIAVRQSEADLALMDEWNVRQHGGSGLTQRVRESTRADADAESATLEFLAQWVPPRKS
                     PMCGNSICQDRRFLARWMPALERHFHYRNLDVSTVKELCQRWAPQIAAGFSKESAHLA
                     MSDIHDSINELRYYREHFLRLA"
     gene            1039..>2405
                     /locus_tag="VHE37_08625"
     CDS             1039..>2405
                     /locus_tag="VHE37_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVT35635.1"
                     /translation="MQRDGDVLNTIGHTPLVELKNLDTGKCRLFVKLENQNPTGSIKD
                     RIGLSMIDAAEREGKIKPGGTLIEATAGNTGLGLALVAAQKGYQLILVIPDKMAQEKV
                     LHLKALGADCRITRSDVGKGHPDYYQDIAERLSKEIPGSFYVNQFSNPANPLAHEATT
                     GPEIWAQTQHMLDAVVCGVGSGGTLTGLSRYFARVAPHVEMVLADPKGSILVDVVNTG
                     KPQKQPGSWLVEGIGEDFIPPVCDLSRVKTAYEIPDAESFNTAREVLRKEGILGGSST
                     GTLVAAALRYCREQSAPKRVVTFVCDSGNKYLSKMYNDFWMFEQGLLARPREGNLLDL
                     IVRRYAEGSAVTVRAADTLLHAYRRMKLFDVSQLPVMDGERIAGIVDESDLLMHVQAD
                     PARFNDTVSTAMVTKVETLAPGAPVSDLVPIFARDHVAIVADHGKLVGLITRIDLLNY
                     LRKQLG"
BASE COUNT          438 a          766 c          801 g          400 t
ORIGIN      
        1 atccgcgccg acgagggcga tctctacgag ctgttcggaa acctgctcga caatgcggcc
       61 aaatggtgca ggcaccaggt tcgggtcgtg gcggcgcatc agggcaaccg cgtccatctt
      121 ctcgtcgagg acgacggcgc cggtttcccg gaagatgccg aggcgctgct cgagcgcggc
      181 gtgcgcgccg acacgcgcgt ggccggccag ggcatcgggc tgtcggcggt ggccgagctg
      241 gtcgccgcgt acgaagggcg catcacgctc gggcgatcag ccacgcttgg cggcgcgttg
      301 gtggaaatca cgctctgaag gcgcaaaaaa ccggcaccgc atctccattt ttgatgttaa
      361 taattattgt ctgtttttac tttaagacaa tatttaataa catcgaaaat gccagtgcgg
      421 tggagtggtt ttggcctcag gcgagccgca ggaaatgctc gcggtagtag cgcagctcgt
      481 tgatcgaatc gtggatgtcg ctcatcgcca ggtgcgccga ctccttgctg aagccagcgg
      541 cgatctgcgg cgcccagcgc tggcacagtt ccttcaccgt gctgacgtcg agattgcggt
      601 aatggaagtg ccgctccagc gccggcatcc agcgcgcgag gaagcggcgg tcctggcaga
      661 tggagttgcc gcacatcggc gatttgcgcg gcggcaccca ctgcgcgagg aattccagcg
      721 tggcgctttc ggcgtcggca tcggcccgcg tgctctcgcg cacgcgctgc gtcagcccgg
      781 agccgccgtg ctggcgcacg ttccactcgt ccatcagcgc caggtccgct tccgactggc
      841 gcaccgcgat caccggccct tccgccagca cgttgaggtc ctggtcggtg accagcgtgg
      901 cgatctcgat gatgcggtgc tgctccggcg agaggccggt catctcgagg tcgatccaga
      961 tcaggttgct ggcgttggca ttcatgcccg catcgtatgg actaccatgc cggcatacaa
     1021 gaacgatgga ggaacgccat gcaacgcgat ggcgatgtgc tcaacaccat cggccacacg
     1081 ccgctggtgg agctgaagaa cctcgacacc ggcaaatgcc ggctgttcgt gaaactcgaa
     1141 aaccagaacc ccaccggctc gatcaaggac cgcatcggcc tgtcgatgat cgacgccgcc
     1201 gagcgcgaag gaaaaataaa accgggcggc accctgatcg aggccaccgc cggcaacacc
     1261 gggctgggcc tggcgctggt ggcggcgcag aagggctacc agctgatcct ggtcattccc
     1321 gacaagatgg cgcaggagaa ggtgctgcac ctcaaggcgc tgggcgccga ctgccgcatc
     1381 acgcgcagcg acgtgggcaa gggccatccc gattactacc aggacattgc cgagcggctg
     1441 tcgaaggaaa ttcccggctc gttctacgtg aaccagttca gcaacccggc caatccgctg
     1501 gcacacgagg ccaccaccgg gccggagatc tgggcgcaga cgcagcacat gctcgacgcg
     1561 gtggtgtgcg gcgtgggcag cggcggcacg ctcaccggcc tgtcgcgcta cttcgcgcgc
     1621 gtggcgccgc acgtggagat ggtgctggcc gatcccaagg gctcgatcct ggtcgacgtg
     1681 gtcaacaccg gcaagccgca gaagcagccc ggcagctggc tggtggaagg catcggcgag
     1741 gatttcattc cgccggtctg cgatctctcg cgggtgaaga ccgcgtacga gatccccgat
     1801 gcggagagct tcaacaccgc gcgcgaggtg ctgcgcaagg aaggcatcct cggcggctca
     1861 tccaccggca cgctggtggc cgccgcgctg cgctactgcc gcgagcagag cgcgcccaaa
     1921 cgcgtggtca ccttcgtctg cgacagcggc aacaagtacc tgtcgaagat gtacaacgac
     1981 ttctggatgt ttgagcaggg cctgctggcg cggccgcgcg aaggcaacct gctggacctc
     2041 atcgtgcgcc gttacgccga gggcagcgcg gtgacggtgc gcgccgccga cacgctgctg
     2101 cacgcgtacc ggcgcatgaa gctgttcgat gtgtcgcagc tgccggtgat ggatggcgaa
     2161 cgcatcgccg gcatcgtcga cgagtccgac ctgctgatgc acgtgcaggc cgaccccgcg
     2221 cgtttcaacg acacggtcag cacggccatg gtgaccaagg tggagacgct ggcgcccggc
     2281 gcgcctgtgt ccgacctggt gccgatcttc gcccgcgacc acgtggcgat cgtggccgat
     2341 catggaaaac tggtcgggct gatcaccagg atcgacctgc tcaactacct gcgcaagcag
     2401 ctggg
//
LOCUS       DASZZC010000407         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_938524,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000407 DASZZC010000000
VERSION     DASZZC010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938524"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VHE37_08630"
     CDS             <1..1012
                     /locus_tag="VHE37_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HVT35636.1"
                     /translation="VYQGTAAGAENPVPVTTVTGARSVTLNGLANGTRYYFTVAAFNN
                     GGTGPMSNEDSATPAPPPPAAPKGLVATSGDGKAVLSWSASAGAASYAVFQGSASGEE
                     SDTPVKASITATQTTITGLTNGEVYYFVVKARNGGGFSAPSNERPAHPQPPPPPAPKG
                     LTATAGDGKANLSWSAADRAVTYSVLQGPAPGSESAAPVKSGITGTTTTISGLVNGTT
                     YYFVVEAKNGGGISPLSNERAVTPQPPRPAAPKNLALANGDGQVTLNWTAADRAASYS
                     VFVGSAAHAESDTPVQSGITGTSTVVSGLVNGTTYYFVVKAVNGGGTSAASNERAGRP
                     HP"
     gene            complement(1025..>1758)
                     /locus_tag="VHE37_08635"
     CDS             complement(1025..>1758)
                     /locus_tag="VHE37_08635"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVT35637.1"
                     /translation="DSHRGRNNFEGTRTERVYTLVARARIFEQIAQDERVMALCARFM
                     QPGFLLTASQAINIHPGETPQPIHYDDQFYRIPRPRPAISVSTIVAVDAFTAENGGTE
                     MIPASHAWSDEQIAGIYDGLDADAPTVKALERQLVPTQMPAGACVVFLGTLLHRGGAN
                     RSAAPRLAFSNQYCEPWARTQENFFLGIPPQIARDMSPRMQQLLGYDIWPPFMGQVTA
                     RHPLKALREDWIPPVLQAPGADHNS"
BASE COUNT          298 a          579 c          629 g          252 t
ORIGIN      
        1 cgtgtaccag ggcacagcgg caggcgcgga aaacccggtg ccggtgacca cggtgaccgg
       61 cgcgagaagc gtaacgctca acggccttgc caatggcacg cgctactact tcacggtggc
      121 ggcgttcaac aacggtggca cgggcccgat gtcgaacgag gactccgcca cgcccgcgcc
      181 gcccccgccg gcggcgccga aggggctggt ggcaaccagc ggcgacggca aggcggtact
      241 gagctggtcg gcctccgcgg gtgccgcgag ttatgccgtg ttccagggca gcgcttcagg
      301 cgaggagagt gacacgccgg tgaaggcatc gatcaccgcc acccagacca ccatcaccgg
      361 cctcaccaat ggcgaggttt attacttcgt ggtcaaggcg agaaatggcg gcggtttcag
      421 cgcgccgtca aacgagcggc cggcccatcc gcaaccgccg ccgccaccgg caccgaaagg
      481 cctcacggcg acggcgggcg atggcaaggc aaacctgagc tggtcggcgg ctgaccgtgc
      541 ggtgacttat tccgtcctgc aaggccctgc accgggatca gagagcgcgg cgccggtgaa
      601 gagcggcatc accggcacca ccaccaccat cagcgggctg gtcaatggca ccacctatta
      661 cttcgtggtg gaggcgaaga acggcggcgg catcagcccg ctgtcgaacg agcgcgcggt
      721 gacgccgcag cctccgcgcc cggcggcacc gaagaacctg gcgctggcca acggcgacgg
      781 gcaggtgacg ctcaattgga cggcggcgga tcgcgcggcc agctattcgg tcttcgtcgg
      841 ctccgccgcg cacgcagaaa gcgacacgcc ggtgcagagc ggcatcaccg gcaccagcac
      901 ggtggtcagc ggactggtca acggcaccac gtactatttc gtggtgaagg cggtgaacgg
      961 cggcggcacc agcgctgcgt ccaacgagcg agcagggcgg ccgcatccgt aacggaccgc
     1021 gtgatcagct gttgtgatcg gcgcccggtg cctgcagcac cggcgggatc cagtcctcgc
     1081 gcagcgcctt gagtggatgc cgcgcggtga cctggcccat gaacggcggc cagatgtcgt
     1141 agcccagcag ctgctgcatg cgcggcgaca tgtcgcgcgc aatctgcggc ggaatgccga
     1201 ggaagaaatt ctcctgcgtg cgcgcccatg gctcgcagta ctggttggag aacgccagcc
     1261 gcggcgcggc gctgcggttg gcgccgccgc gatgcagcag cgtgccgaga aacaccacgc
     1321 aggcgccggc gggcatctgc gtgggcacca gctggcgctc cagtgctttc accgtcggcg
     1381 cgtcggcgtc gagtccgtcg tagatgccgg cgatctgctc atcgctccag gcgtggctgg
     1441 cgggaatcat ctcggtgccg ccgttttccg cggtgaaggc atcgacggca acaatcgtcg
     1501 acacgctgat cgccgggcgc ggccgcggga tgcggtagaa ctggtcgtcg tagtggatcg
     1561 gctgcggcgt ctcgccgggg tggatgttga tggcctggct ggcggtgagc aggaagcccg
     1621 gctgcatgaa gcgcgcgcac agcgccatca cgcgctcgtc ctgcgcgatc tgctcgaaga
     1681 tgcgggcgcg cgccaccagc gtgtacacgc gctcggtgcg cgtgccttcg aagttgttgc
     1741 gcccgcgatg gctgtcga
//
LOCUS       DASZZC010000408         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_289465,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000408 DASZZC010000000
VERSION     DASZZC010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289465"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VHE37_08640"
     CDS             complement(<1..716)
                     /locus_tag="VHE37_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35638.1"
                     /translation="MLQLKKPRGNSMRSLTCGLLAALLVVSCGGGGGSAGPAQAPSPA
                     VPPAQDSFYLPFFTGDGRLQLINPATPTAAPSTLDSGLDVQQAERFARGTYQASGPVV
                     LSDAHFSDLVYIKQGHVYRMGLGGLPAVQQISAIDNACQLLPLFADYKNFDHSLLRVV
                     VADPGQTCSSSSFSTRVVIGLAAGTSDPGVTVGRVVALARDYNADGSLAGEVVNESGT
                     IRFYDANFANPQNIAANVADV"
     gene            complement(798..>1675)
                     /gene="cysG"
                     /locus_tag="VHE37_08645"
     CDS             complement(798..>1675)
                     /gene="cysG"
                     /locus_tag="VHE37_08645"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HVT35639.1"
                     /translation="LERGQRRALWEKFLDSAAPARLYAGDEAGAEEILQQLAQESSAR
                     GEVYLVGAGPGDPDLLTLRALRLMQQCDVVLYDRLIAPAILNLVRRDAERMFVGKQAH
                     HHVVPQDQINAELVRLARQGKRVLRLKGGDPFIFGRGGEEIDTLAAAGIPFEVVPGIT
                     AASGCSAYAGIPLTHRDYAQSCVFVTGHARAGGELDLHWDQLARRGQTVVIYMGLQTL
                     PALCAKLIEHGLPRDWPAAVIEEGTSPRQRVFASTLADLPARVAAEKVEGASLLIVGE
                     VVKLRERLTWYRAGR"
BASE COUNT          299 a          558 c          535 g          283 t
ORIGIN      
        1 acgtcggcga cattggcggc aatgttctgt ggatttgcga aattggcatc gtagaaacgt
       61 atggtgccgc tctcgttgac cacctctcct gccaggctcc cgtcagcgtt gtagtcacgc
      121 gcgagcgcta ccacccggcc aacggtgacg cccgggtcgg aagtaccggc ggccaggccg
      181 atgaccacgc gggtactgaa tgacgaactg ctgcaggtct ggcccggatc ggccacgacc
      241 acgcgtaaca acgaatgatc gaaattcttg taatcggcaa acagcggcag caactggcag
      301 gcattgtcga tggcggaaat ttgctggaca gccggcaggc cgcccagccc catgcggtag
      361 acgtgcccct gcttgatgta caccagatcg gaaaagtgtg cgtcactcaa tacgaccggc
      421 ccgctcgcct gataggttcc acgtgcgaaa cgttccgcct gttgcacgtc gaggcccgaa
      481 tcgagagtcg atggagcagc ggttggcgtg gccggattga tcagctgcaa cctgccgtcg
      541 ccggtaaaga aaggcagata gaacgaatcc tgcgccggcg gaacggcagg tgacggcgct
      601 tgcgctggcc cggcactgcc accaccgccc ccgcaagaaa caaccagcaa agcggccagc
      661 aggccgcaag tcagactacg catggaattc ccccttggtt ttttcagttg cagcaaggtt
      721 ccctagacct gctgtgcacc cgtggtgcca gtgtataaat tcggctcgcg cgcaggaatg
      781 cggatagaga tttttttcta gcgccccgcc cggtaccacg tgagcctctc gcgcagcttc
      841 accacttcgc cgacgatcag caggctggcg ccttccacct tttccgcggc tacacgcgcc
      901 ggcaaatcag ccagcgtcga agcgaacacg cgctgccgcg gcgaagtccc ctcctcgatt
      961 accgctgctg gccagtcgcg cggcagtccg tgctcgatga gcttcgcgca cagcgccggc
     1021 agtgtctgca aacccatgta gatgaccacg gtctggccgc ggcgcgcgag ctggtcccag
     1081 tgcagatcga gctcgccgcc ggcacgcgcg tggccggtga cgaacacgca gctctgcgcg
     1141 tagtcgcggt gcgtgagcgg gatgccggcg tatgcggagc agccgctggc ggcagtgatg
     1201 ccgggcacca cctcgaacgg tatgccggcc gcggccagcg tgtcgatctc ctcgccgcca
     1261 cggccgaaga tgaagggatc gccgcccttg agccgcagca cgcgcttgcc ttgccgcgcc
     1321 aggcgcacca gctcggcgtt gatctggtcc tgcggcacca cgtggtggtg cgcctgcttg
     1381 ccgacgaaca tgcgctcggc gtcgcggcgc accaggttga ggatggccgg cgcgatcagg
     1441 cggtcgtaca gcaccacgtc gcattgctgc atcaaccgta gcgcacgcag cgtcagcagg
     1501 tctggatcgc cggggccggc gccgaccagg tacacctcgc cgcgtgccga ggattcctga
     1561 gcaagctgct gcaggatttc ttcggcgccc gcctcgtcgc ccgcgtacag ccgcgcgggc
     1621 gcggcgctgt cgaggaattt ttcccacagc gcgcgccgct ggccgcgctc gagct
//
LOCUS       DASZZC010000409         5995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1060399,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000409 DASZZC010000000
VERSION     DASZZC010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060399"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VHE37_08650"
     CDS             <1..914
                     /locus_tag="VHE37_08650"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HVT35640.1"
                     /translation="VADPHTDEKLRSRLTRLQDARRFATAQLTLPDNGSYTQYADLKR
                     PYAVWNVFAAPEFSLEPVQHCFLVIGCLSYRGYFAKDDAEAEAARLSAAGAEIYVAGI
                     PAYSTLGWFDDPVLNTMMHWSDEVLVATLFHELTHQKFYLKGDTAFDESLASFVEDEG
                     LKQYQDARGWHSDAYVIYQKRQQQVTALMLDARARLAALYAQKLPADETRRRKQAEFD
                     RLRASYRALRDGEWHGYDGYDRMFNGELNNARMLPYGLYDQWQPAFAALYRQSGQQWP
                     AFYDAVKKLAALPDEQRKEALNKLAGL"
     gene            complement(942..1907)
                     /locus_tag="VHE37_08655"
     CDS             complement(942..1907)
                     /locus_tag="VHE37_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT35641.1"
                     /translation="MTARSHHYAGRFRNRYGGGAPGPSFGFIKFILKLAQVSRSGGAL
                     PLLKPEVESLRNNRSESTLTWIGHSTFLLQHAGINLITDPHLTQRASPFRHAGPARFT
                     PPALEFSELPKLDIALVSHDHYDHLDEQTVTRLAREHPHLVFVVPRGLKAWFKRRHIH
                     KVVELDLWQHSPRIGGVTVHCVPVQHFSGRGPHDRNATLWCGYVVEIDGRKIFFAGDT
                     GYSRDFTDIHAKHGEMDLSLLPIGAYEPREFMAHVHVDPAEAVRIHQDLHSRQSVAMH
                     WGVFRLTLEPLDEPPVKLAQALAAQGIAAEKFRVMQIGETLKDFL"
     gene            1957..3327
                     /gene="cysS"
                     /locus_tag="VHE37_08660"
     CDS             1957..3327
                     /gene="cysS"
                     /locus_tag="VHE37_08660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HVT35642.1"
                     /translation="MQLHDTYTGGKKAFVPLDPQHVTLYLCGPTVYSYAHIGNARPAV
                     VFDVLARVLRRKYPKVTYARNITDIDDKINAAASKQGVDISVITDKFAAIYHEDMAAL
                     GVLPPDAEPRVTQHLPQIVAMIQRLIDNGHAYAAEGHVLFHVPSYAEYGQLSKRDTKE
                     LLAGARVETAPYKKDAGDFVLWKPSTPDLPGWDSPWGRGRPGWHIECSAMTEAIFGET
                     IDIHGGGLDLIFPHHENERAQSVCAHGGKVFARYWVHNGFLTMNSGKMSKSVGNVLLV
                     HELLKHAPGEAIRLALLTAHYHQPLDWNDDVIPDARKKLDRLYGALRELDGVTAAPGV
                     QAPESFVAALEDDINTPMALAELFELASAANKATDATEKSRLKAQLLDAGSLLGLLQQ
                     KPETWFATGAADAMPATEIEARLAERLQARKDKRWADSDRIRDELKARGVLVEDGKDG
                     QRWRYL"
     gene            complement(3357..4769)
                     /locus_tag="VHE37_08665"
     CDS             complement(3357..4769)
                     /locus_tag="VHE37_08665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT35643.1"
                     /translation="MRNQPPEFANVVLDALTAHIAVLDEHGVIIGVNEPWRRFSEANQ
                     GRSSCYYVGENYLSVCENAARCGGDKTAAALIDGLRSVLRGQRSRFALEYPCHSPDEE
                     RWFIVWVTPCSQGGASRVVVAHEDITARKKAELALARAELTLRKILDTLPVGVWVMDR
                     NGHVIQGNAAGQKIWGVSAAQAPELLADYKGWWLDSGKPVAAAEWAAARAISRGETSI
                     EEKILIEGFDGSRKIILNSSVPLHDADNKISGAIIVNQDITARHEADEQLRRAKLALE
                     QANRELQESLQREKAAAQTDELTGLCNRRHFFSLGGQLFKVALRYGTPLAVVMLDVDH
                     FKRINDTHGHQAGDRTLSEIARRAAGQLRAADLLARYGGEEMVLLLPESTAAQALQVA
                     EGIRTAVAAQPVPCGQTPVPVSLSAGIAEIDALDGSLDAIIRRADRALYEAKASGRNC
                     CRIFGPQTALRSTESTATEF"
     gene            complement(4874..5458)
                     /locus_tag="VHE37_08670"
     CDS             complement(4874..5458)
                     /locus_tag="VHE37_08670"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HVT35644.1"
                     /translation="MSTRRCHWAGSNPLMQAYHDQEWGRPQHDDRVLFEFLVLEGAQA
                     GLSWSTVLNKRAHYRKALDNFEPAKIARYSDAKLEKLLLDPGLIRNRLKIHSMRTNAQ
                     AFLKIQNECGSFDAWLWAFVDGKTIVNRPRGREGVPARTELSDRISKELLKRGFKFVG
                     STIVYAYMQAVGLVDDHMAGCFSAGRRRSATKPR"
     gene            complement(5455..>5995)
                     /locus_tag="VHE37_08675"
     CDS             complement(5455..>5995)
                     /locus_tag="VHE37_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT35645.1"
                     /translation="SQHGQDSVNAGMPAFIIAVLTLLMFQLRSFSRTLLVVATAPLGM
                     IGVTLALLLTGKPFGFVAMLGTIALSGMIMRNSVILVDQIEHDIANGSAPEVAVVDAA
                     VRRLRPIVLTALAAVLAMIPLLLESIFFAPMAAAIMGGLIAATALTLLFLPALYAAWF
                     RLPRDTHAHLQPVSVTAQP"
BASE COUNT         1053 a         1989 c         1959 g          994 t
ORIGIN      
        1 tggtggctga cccgcatacc gatgaaaagc tgcgctcgcg gctgacgcgc ctgcaggacg
       61 cgcgccgctt cgccacggcg cagctcacgc tgcccgacaa cggcagctac acgcagtacg
      121 ccgatctcaa gcggccctac gcggtgtgga acgtgttcgc cgcgccggag ttttcgctgg
      181 agccggtgca gcactgcttc ctggtgatcg gctgcctgtc gtaccgcggc tactttgcca
      241 aggacgacgc cgaggccgag gccgcgcgcc tgtcagccgc gggcgccgag atctacgtgg
      301 ccggcattcc cgcctactcc acgctgggct ggttcgacga cccggtgctc aacaccatga
      361 tgcactggtc cgacgaggtg ctggtggcca cgctgttcca cgagctcacc caccagaagt
      421 tctatctgaa gggcgacacc gcattcgatg aatcgctggc cagcttcgtc gaggatgaag
      481 gactgaagca gtaccaggac gcccgcggct ggcacagcga tgcctatgtg atctaccaga
      541 agcgccagca gcaggtcacc gcgctgatgc tcgacgcgcg cgcgcgcctg gccgcgctgt
      601 atgcgcagaa gctgcccgcc gacgaaacgc gccggcgcaa gcaggcggag ttcgaccgcc
      661 tgcgcgccag ctaccgcgcg ctgcgcgacg gcgagtggca cggctacgat ggctacgacc
      721 gcatgttcaa cggcgagctg aacaacgcgc gcatgctgcc gtacggtctc tacgaccagt
      781 ggcagccggc tttcgccgcg ctgtatcgcc agtcgggcca gcagtggccg gcgttctacg
      841 acgcggtgaa gaaactggcg gcgctgccgg acgagcaacg caaagaggcc ttgaacaaac
      901 tcgccggcct ataggagccg ccttgcgcgc aaggcgcgct cctacagaaa atccttcagc
      961 gtctcgccga tctgcatcac gcggaatttt tccgcggcga tgccctgcgc cgccagcgct
     1021 tgcgccagtt tcaccggcgg ctcgtccagc ggctccagcg tcaacctgaa cacgccccag
     1081 tgcatggcca ccgactggcg gctgtgcaga tcctggtgga tgcgcactgc ctccgccgga
     1141 tcgacatgca catgggccat gaactcgcgc ggctcatacg cgccgatcgg cagcagcgag
     1201 agatccatct cgccatgctt cgcatggatg tcggtgaagt ctcgcgagta gccggtgtcg
     1261 ccggcgaaaa agatcttcct gccgtcgatc tccaccacgt agccgcacca cagcgtggcg
     1321 ttgcggtcgt gcggaccgcg cccggagaaa tgctgcaccg gcacgcaatg cacggtcacg
     1381 ccgccgatac gcggcgagtg ctgccataaa tcgagttcca ccaccttgtg gatgtgccgc
     1441 cgtttaaacc aggccttgag cccgcgcggc accacgaaca ccaggtgcgg atgctcgcgc
     1501 gccaggcgcg tcaccgtctg ctcgtcgagg tggtcgtagt ggtcgtgcga gaccagcgcg
     1561 atgtcgagct tcggcaattc ggaaaattcc agcgcgggcg gcgtgaagcg cgccggcccg
     1621 gcatgccgga acggcgaggc gcgctgcgtc aaatgcggat cggtgatgag gttgatgccg
     1681 gcatgctgca gcaggaaggt gctgtggccg atccaggtga gcgtgctctc gctgcggttg
     1741 ttgcgcagcg actcgacctc gggcttgagc agcggcagcg cgccgccgct gcgcgacacc
     1801 tgcgccagct tcaggatgaa cttgatgaag ccgaaactgg gtcccggcgc accgccaccg
     1861 tagcggttgc ggaaacgtcc ggcgtaatgg tgcgagcgtg cggtcatgcg gtggtggttc
     1921 gggtatcctt ccgcgtcatt tttacatagc gcggccatgc aactgcacga cacctacacc
     1981 ggcggcaaaa aggcattcgt accgctcgat ccgcagcacg tcacgctcta cctgtgcggc
     2041 cccacggtgt actcctacgc acacatcggc aacgcgcgcc cggcggtggt gttcgacgtg
     2101 ctggcgcgcg tgctgcgccg caagtacccc aaggtgacct acgcgcgcaa catcaccgac
     2161 atcgacgaca agatcaacgc cgccgcgtcc aagcagggcg tggacatctc cgtcatcacc
     2221 gacaagttcg cggcgatcta ccacgaggac atggccgcac tcggcgtgct gccgcccgac
     2281 gccgaaccgc gcgtgacgca gcacctgccg cagatcgtgg ccatgatcca gcggctgatc
     2341 gacaacggcc acgcctacgc tgccgaaggc catgtgctgt tccacgtgcc gagctacgcc
     2401 gagtacggac aactgtcgaa gcgcgacacc aaggagctgc tcgcgggcgc gcgcgtggaa
     2461 accgcgccgt acaagaagga cgccggcgat ttcgtgctgt ggaagccatc gacgcccgat
     2521 ctgcccggct gggattcacc ctggggccgc gggcggccgg gctggcacat cgagtgctcg
     2581 gccatgaccg aggcgatctt cggcgagacc atcgatatcc acggcggcgg gctggacctg
     2641 atcttcccgc accacgagaa cgagcgcgcg cagtcggtat gcgcgcacgg cggcaaagtc
     2701 tttgcgcgct actgggtgca caacggcttc ctcaccatga acagcggcaa gatgtccaag
     2761 agcgtgggca acgtgttgct ggtgcacgaa ctgctcaagc acgcgccggg agaagcgatc
     2821 cgcctggcct tgctcaccgc tcactaccac cagccgctgg actggaatga cgacgtgatt
     2881 cccgatgcgc gcaaaaagct cgaccgactg tacggcgcgc tgcgcgagct ggacggcgtg
     2941 acggcggcgc ccggtgtgca ggcgccggag agctttgtcg ccgcgctgga ggacgacatc
     3001 aacacgccca tggcgctggc cgagctgttc gaactcgctt ccgccgccaa caaggcgacc
     3061 gatgccacgg agaaatcccg cctcaaggcg cagttgctcg acgcaggatc attgctgggc
     3121 ctgctgcagc agaagccgga aacctggttt gccaccggtg cggcggatgc gatgcctgct
     3181 acggaaatcg aagcaaggct ggccgagcgc ctgcaggcac gcaaggacaa gcgctgggcc
     3241 gactccgacc gcatccgcga cgagctgaag gcgcgcggcg tgctggtgga agacggcaag
     3301 gacggacagc gctggcgcta cctgtagccg cggcgcgacc acgacgtttc gaggaatcag
     3361 aattccgttg cggtcgactc cgtggaacgc aacgccgtct gcggaccgaa aatccggcag
     3421 caattgcggc cgctggcctt ggcctcgtaa agcgcgcggt ccgcgcggcg gatgatggcg
     3481 tccaggctcc cgtcgagcgc gtcaatttca gcaatgccgg cgctgaggct caccggtacc
     3541 ggcgtctgcc cgcacggcac cggttgtgcc gccaccgccg tgcggatgcc ttctgccacc
     3601 tgcaaggcct gtgcagcagt gctttccggc agcagcaaga ccatttcttc cccgccgtag
     3661 cgcgcgagta gatcggcggc ccgcagctgc cccgcagcgc gacgggcgat ttcgctcaac
     3721 gtccggtccc cggcctggtg cccatgggtg tcgttgatcc gcttgaagtg gtccacgtcg
     3781 agcatcacga ccgcgagcgg cgtgccgtag cgcaacgcca ccttgaacag ctgtccgccc
     3841 aggctgaaga aatgacgacg gttgcacaga ccggtcagct catcggtctg cgccgcggcc
     3901 ttttcgcgct gcaggctttc ctggagttcg cggttggcct gctccagcgc aagcttggcg
     3961 cgccggagct gctcgtcggc ctcgtgccgc gcggtgatgt cctggttgac gatgatggcg
     4021 ccgctgatct tgttgtcggc atcgtgcaag ggaaccgacg aattgagaat gatcttgcgg
     4081 ctgccgtcaa agccctcgat caggatcttc tcctcgatgg aggtctcgcc ccggctgatc
     4141 gcgcgcgctg ccgcccattc cgcagcagcc acgggcttgc cggagtccag ccaccagccc
     4201 ttgtagtcgg cgagcagttc gggtgcctgg gcggcggaaa caccccagat tttctgtcct
     4261 gctgcattgc cctggatcac gtgtccgtta cggtccatta cccagacgcc gaccgggagc
     4321 gtgtccagaa tcttccgcaa ggtcagttcg gcacgtgcaa gtgcaagctc cgccttcttg
     4381 cgggcagtga tgtcctcatg ggccaccacg acacgcgacg caccgccctg actgcacggc
     4441 gtgacccaga caatgaacca gcgctcttca tcgggcgaat gacagggata ctccagggca
     4501 aaccggctgc gctggccccg cagcaccgac cgcaggccgt cgatcagggc ggcggcagtc
     4561 ttgtctccgc cgcaacgggc tgcgttttcg cagaccgaca ggtagttctc gcccacgtag
     4621 tagcagctgc tcctgccctg gttggcttcg gaaaagcgcc gccagggctc gttgacgccg
     4681 atgatcacgc cgtgttcgtc gagcacggcg atatgtgctg tcaatgcatc cagcacgaca
     4741 ttggcaaatt ccggaggttg attgcgcatg cggggtcagt ttcctgacca gaccatagcg
     4801 accgcgggca agggcggcat tgacgcaggt cagttccggg cgcggctgcc ggctggcgca
     4861 gtcgctcaac gcgtcaacgc ggcttcgtgg ctgatcgtct gcggcctgcc gagaaacaac
     4921 cggccatgtg gtcatcgacc aggccaaccg cctgcatgta ggcgtagacg atcgtactgc
     4981 ccacgaactt gaacccgcgc ttgagcagtt ccttgctgat gcggtcggac agctctgtgc
     5041 gcgccggcac gccttcgcgc ccgcgcggac ggttgacgat agtcttgccg tcgacaaacg
     5101 cccatagcca ggcatcgaaa ctgccgcact cgttctggat cttgaggaac gcctgcgcat
     5161 tggtgcgcat cgaatggatc ttcaggcggt tgcggatcag gccgggatcg agcagcagct
     5221 tctccagctt cgcatcgctg tagcgcgcga tttttgccgg ctcgaagttg tccagcgcct
     5281 tgcggtaatg cgcgcgcttg ttcagcacgg tgctccagga gaggccggcc tgcgcgcctt
     5341 ccagcaccag gaactcgaac agcacgcggt cgtcgtgctg cggacggccc cattcctggt
     5401 cgtggtaggc ctgcatcagc gggttgctgc ccgcccagtg gcagcgccgg gtgctcatgg
     5461 ctgcgcggtg acgctgaccg gttgcaggtg cgcgtgcgtg tcgcgcggca gccggaacca
     5521 ggccgcgtac agcgccggca ggaacagcag cgtgagcgcg gtggcggcga tcaggccgcc
     5581 catgatggcg gcggccatcg gcgcgaagaa gatgctttcc agcagcaacg gaatcatcgc
     5641 cagcaccgcg gccagcgccg tgagcacgat gggccgcagc cggcgcacgg cggcgtcgac
     5701 caccgcgact tccggcgcgc tgccgttggc gatgtcgtgt tcgatctggt ccaccaggat
     5761 cacgctgttg cgcatgatca tgccgctcaa tgcaatggtg ccaagcatgg cgacgaagcc
     5821 gaacggcttg ccggtgagca gcagcgccag cgtgacgccg atcatgccca gcggcgcggt
     5881 ggccaccacc agcagggtgc gcgagaagct gcgcagctgg aacatcagca gcgtcagcac
     5941 ggcgatgatg aacgcgggca tgccggcgtt gaccgaatcc tgcccatgct ggctt
//
LOCUS       DASZZC010000410         8058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_939523,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000410 DASZZC010000000
VERSION     DASZZC010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939523"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..599)
                     /locus_tag="VHE37_08680"
     CDS             complement(51..599)
                     /locus_tag="VHE37_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HVT35646.1"
                     /translation="MAEAAVEAEEGAAPAAAPKKKSKLPLIIGLVVVLAGAGGGAYFF
                     MGKKSSGTADAHGAAAEGHEAAAGDEHGGAAKGNVNFIDFAPPFVVNLDDEEANRYLQ
                     VDMQARGADAHALDGVKDRMPRIRNAILLLLGQQKYHDINTREGKEKLQAQVLAEVQK
                     IMTEETGKPVVDAIYFTSFVMQ"
     gene            897..1412
                     /gene="fliS"
                     /locus_tag="VHE37_08685"
     CDS             897..1412
                     /gene="fliS"
                     /locus_tag="VHE37_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HVT35647.1"
                     /translation="MAGKINAAPIRGLKKFPPAPMYGLNAKHTAAPPETSAMSTPYPF
                     AALRHYNEVGNQASIEASPHKLIDMLLGGALDRLATARGCMLRGDRSGKLAAVSSVVS
                     IVEHLRLSLDMQAGGELAQNLSRLYDYMVRRLLKANADDSPVMVEEVSGLVRELKLGW
                     EAMPAVMPTRH"
     gene            1450..1980
                     /locus_tag="VHE37_08690"
     CDS             1450..1980
                     /locus_tag="VHE37_08690"
                     /inference="COORDINATES: protein motif:HMM:NF028133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVT35648.1"
                     /translation="MNGQPLSYADFLPLAWAPADAPLTPHEVEALSNANQEVLAMVAA
                     IENHRKPPDDEATLDNELARLHQKVDLLLELVSAMAHAQLPQPPPVAVRISSQDMVCQ
                     GFGAQPGPGIVSIYLHRCMAQPLRLPAQLDTQHGDGSARMQFLPLDESCTAALERHVF
                     LHHRRSVAEARLSQKR"
     gene            2047..2682
                     /locus_tag="VHE37_08695"
     CDS             2047..2682
                     /locus_tag="VHE37_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT35649.1"
                     /translation="MRVILADTQVLVRAGLRKLIEALRGVEVVGETGDGQELLEMIGH
                     LRPEVVVTEVDLVHIRGIDALQQAQRHYPEVNFLMLSSQHEARHVRSALKVGARGFLV
                     KDAELPELELALRAVARGQTYLSPSISHNALNARRHQRAEEQVALTPRQRQVLQLVGR
                     GRTTKEIAGLMGVSPKTVETHRMRMMQSLGLYGTSALMRFAIRVGADGGEF"
     gene            complement(2695..4068)
                     /gene="fliD"
                     /locus_tag="VHE37_08700"
     CDS             complement(2695..4068)
                     /gene="fliD"
                     /locus_tag="VHE37_08700"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HVT35650.1"
                     /translation="MASPITGTITSAGIGSGLDVNSLVSQLVAAEKAPDSDRITKGQA
                     VDNAKLSALGTFKSAISALQTAAQTLEGSSGLGKIAANTSDNTIVTATAASGTLSAFY
                     DVEVVSLATASKQVSGSFANSSTSVGDGTITITVGSKSFSVTTAPGSDTLANLRDAIN
                     SADDNTGVNASIVSDSNGAHLVLTSRGTGTANAVSVSSSATADSSNFFTTTQSQGAVD
                     AHIKVDGGDVYSSSNTVSNAIDGVTLNLVKAAPGTTVSLTLSANTSSAATAVQSFVNT
                     YNAVIASMASQTKYDASTNTAGALLGDAAASGFMTQLANLVGGNASGTGSVFSNLSDL
                     GITTQSDGTLKVNTDKLNAALTQDADSVQKLFSADGGYASAIDKLVGTFTASDGGIQA
                     ETDSLNSQLKDLQRQSDDLDARMQTVQARYQAQFSALDTLIAQMKSTQDYLTQQLAAI
                     ANTTKSS"
     gene            complement(4092..4478)
                     /locus_tag="VHE37_08705"
     CDS             complement(4092..4478)
                     /locus_tag="VHE37_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HVT35651.1"
                     /translation="MSTPVSEMTPVLAAATTSTKRGDASPRDLSLPTTKTQATNADAQ
                     GAGAAPAPDNAALQKAVDELNRHFASIRTDLRFSVEKDLNVIVVAVVDSKDGTVLRQI
                     PSEEALRIARVLAEQRSGANLVEAVA"
     gene            complement(4562..5719)
                     /locus_tag="VHE37_08710"
     CDS             complement(4562..5719)
                     /locus_tag="VHE37_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HVT35652.1"
                     /translation="MASVINTNVMSLNAQRNLNSSQMSLATAMQRLSSGLRINSAKDD
                     AAGLAISDRFTAQINGLDQASRNANDGISLTQTAEGALAEVTNNLQRIRELAVQSRNG
                     SNSSTDRAALNSEAQQLKAEVDRVAQQTQFNGVNLLDGSFSNQAFQVGANAGQTIAVS
                     SIVDATSVNLGSYTSATVTGVAATTLTAITAGDLTIDGGNGNGAISVGGVGAAASATV
                     RAGQIRDAINSVADQTGVYAVNDTATTVQLTSTSGTGITIALSGTGTTATTGLTAATT
                     ATATNTGFSTLSLTTSAGADTAIKQMDAALAAVSTARGNLGALQNRFQSVVNSLSTAS
                     ENLSAARSRIMDADFAKETAAMTRSQILQQAGTAMLAQANAAPQNVLSLLK"
     gene            complement(5977..6879)
                     /gene="flgL"
                     /locus_tag="VHE37_08715"
     CDS             complement(5977..6879)
                     /gene="flgL"
                     /locus_tag="VHE37_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HVT35653.1"
                     /translation="MRISTAGLHQQGVDNILKQQTVLADVQNELSTGKKIITASDNPA
                     GAARALVLDQTAADITRWQTNVGTAQDRLGLEETALSGTNDVLTQLRSLAIQANSATQ
                     SDASRSALATQMTQLYQQLLGYANSDDGTGRYLFGGSNDAQPPFTDGASGVSYSGDQT
                     QRLLDIGHNRAVADGDTGSAVFQNTGSGDLFATVKNLIALVQTPGGATQAQYSASLDA
                     LQTAGDHISDMRAGVGARLAMLDDATSSLAQQSTSTAKSLSDLRDVDVTDAASRLALA
                     QTLLQAAQQSYLKVQGLSLFDYLR"
     gene            complement(6889..>8058)
                     /locus_tag="VHE37_08720"
     CDS             complement(6889..>8058)
                     /locus_tag="VHE37_08720"
                     /inference="COORDINATES: protein motif:HMM:NF044995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HVT35654.1"
                     /translation="SRSTSLTLTEDDYHSGRLQLALTSGGDITAQVSGGVIGGLLDAR
                     REVLDPAEEQLGRMAIAITSAVNDQNHAGIDLRGQLGGDLFAPITGTALQASANTGNA
                     TAQVGIADAGAITGDDYQLKYDGSSWSMTNASTGAAVALSGSGTAADPLTGAGISITL
                     AGTPAAGDRFLVEPARQAASQVQLATTDTSAIAAAAPVSASAATGNTGTATISAPTVL
                     DAGNAALMSGVTIAFTSATTYSINGSGSYTYSAGGNIDFNGWRVQIKGAPATGDTFSI
                     SANGANSGDNRNARAMSALSSAGVLDGGLTSVTSANAALVSSTGSTAQQASLRANAQN
                     AILGDTQKQRDSVSGVNLDEEAADLVRFQQAYQAAARIIQVADTVFQSLLQAAQG"
BASE COUNT         1156 a         2603 c         2816 g         1483 t
ORIGIN      
        1 ctgcgcattg cggttctccg tcgcttgagc gaagtgagca taggcaggcc ctactgcatc
       61 acgaagctgg tgaagtagat ggcatcgacc accggtttgc cggtttcctc ggtcatgatc
      121 ttctgcacct cggccagcac ctgcgcctgc agcttctcct tgccctcgcg cgtgttgatg
      181 tcgtggtact tctgctggcc cagcagcagc aggatggcgt tgcggatacg cggcatgcgg
      241 tccttcacgc cgtcgagcgc gtgcgcgtcg gcgccgcgcg cctgcatgtc cacctgcagg
      301 tagcggttgg cttcctcgtc gtcgaggttg accacgaaag gaggcgcgaa atcgatgaag
      361 ttgacgttgc ccttggccgc gccgccgtgc tcgtcgccgg ccgcggcttc gtggccttcc
      421 gccgcggcgc catgcgcgtc ggccgtgccg gacgatttct tgcccatgaa gaaataggcg
      481 ccgccaccgg cgccggccag caccaccacc aggccgatga tcagcggcag cttgctcttc
      541 ttcttcggcg ccgccgcggg tgctgcgcct tcttccgctt ctactgctgc ttccgccatg
      601 gctcgccgtg ctcgcgtgat gatttacgca ggcgcaggag caagtcatgt gccacggaaa
      661 aaagcttatg gatgagatcg ttgcgcaggc gcgccggaat cttgactgcg tgttctgcgc
      721 agcggggcgc cggaagtccg gcgcgtacgc ccggcgtccg cggctgatcc ggcaacgggc
      781 ctcgatgcgc gcgtggagcc aaaaacgtgc ttaccgcgcc gctcttatct ctgttatata
      841 aaattgtata tttataaata taactaattt tttataacat cgctccgggc cttgcggtgg
      901 ctgggaaaat caatgccgcg ccgattcgcg ggctcaagaa atttccgccg gcgccgatgt
      961 atggcctgaa cgcgaaacac accgcagcac caccggaaac ttcagccatg tccacgccgt
     1021 atcccttcgc cgccctgcgc cactacaacg aagtcggcaa ccaggcttcg atcgaggcca
     1081 gcccgcacaa gctgatcgac atgctgctcg gcggcgcgct cgaccgcctg gccaccgcgc
     1141 gcggctgcat gctgcgcggc gaccgcagcg gcaagctggc ggcggtgagc tcggtggtat
     1201 ccatcgtcga gcacctgcgg ctgtcgctgg acatgcaggc cggcggcgag ctggcccaga
     1261 acctgtcgcg cctgtatgac tacatggtgc gccgcctgct caaggccaac gccgacgaca
     1321 gcccggtgat ggtcgaggaa gtctccggcc tcgtgcgcga gctcaagctc ggctgggaag
     1381 ccatgccggc ggtgatgccg acgcgccact gaggcgcgtc cgggcacgga actttcatgg
     1441 agcccggcca tgaacgggca accgctgtca tatgccgatt ttctgccact ggcctgggcg
     1501 ccggcggacg cgccgctgac gccgcacgag gtggaagcgc tgtccaacgc caaccaggaa
     1561 gtgctggcga tggtggcggc catcgaaaac caccgcaagc cgcccgacga cgaagccacg
     1621 ctggacaacg agctggcgcg gctgcaccag aaggtcgacc tgctgctgga actggtcagc
     1681 gccatggcgc atgcccagct gccgcagccg ccgccggtgg cagtgcgcat ctcctcgcag
     1741 gacatggtgt gccagggctt cggcgcgcag ccgggtccgg gcatcgtctc catctacctg
     1801 caccgctgca tggcccagcc gctgcgcctg ccggcgcagc tggacacgca gcacggcgac
     1861 ggcagcgcac gcatgcagtt cctgccgctg gacgaaagct gcaccgcagc actggaacgc
     1921 cacgtgttcc tgcatcaccg ccgttccgtc gccgaagcgc gtttgtcgca aaagcgctga
     1981 tctggcacgc tgccgtccgc acaatgcggc ggcaatgaac cactacaaaa ccgaggagcg
     2041 tcacgcatgc gcgtgatcct ggccgacacc caggtgctgg tgcgcgcggg cctgcgcaag
     2101 ctgatcgaag cgctgcgcgg cgtggaggtg gtgggcgaaa ccggcgacgg ccaggaactg
     2161 ctggaaatga tcggccacct gcgcccggaa gtggtggtca ccgaggtcga cctggtccac
     2221 atccgcggca tcgacgccct gcagcaggcg cagcggcact atcccgaggt caatttcctc
     2281 atgctgtcct cgcagcacga ggcgcgccac gtgcgcagcg cgctcaaggt gggcgcgcgc
     2341 ggcttcctgg tcaaggatgc cgaactgccc gagctggagc tggccctgcg cgcggtggcg
     2401 cgcggccaga cttatctttc gcccagcatc tcgcacaacg cgctcaatgc gcgccggcac
     2461 cagcgcgcgg aagaacaggt ggcgcttacc ccgcgccagc gccaggtgct gcagctggtg
     2521 ggccgcggcc gcaccaccaa ggaaatcgcc gggctgatgg gcgtgtcgcc caagacggtg
     2581 gaaacccacc gcatgcgcat gatgcagagc ctgggactgt acggcaccag cgcgctgatg
     2641 cgcttcgcca tccgcgtggg ggcggacggc ggcgagttct agcgcgccgc gccctcagct
     2701 cgacttggtg gtgttggcga tcgccgccag ctgctgggtc aagtaatcct gcgtgctctt
     2761 catctgcgcg atcagcgtat cgagcgcgct gaactgcgcc tggtagcgcg cctgcaccgt
     2821 ctgcatgcgc gcgtcgagat cgtcgctctg ccgctgcaga tccttgagct gggaattgag
     2881 gctgtcggtt tcggcctgga tgccgccgtc gctggcggtg aaggtgccga ccagcttgtc
     2941 gatggcactg gcatagccgc cgtctgcgga aaacagtttc tgcaccgagt ccgcgtcctg
     3001 tgtcagcgcg gcgttgagct tgtccgtgtt gaccttgagc gtgccgtcgc tctgcgtggt
     3061 gatgcccagg tcggaaaggt tgctgaacac gctgccggtg ccgctggcgt tgccgccgac
     3121 caggttggcc agctgggtca tgaagccgct ggcggcggcg tcgccgagca gcgcccccgc
     3181 ggtgttggta ctggcgtcgt acttggtctg cgacgccatc gacgcgatca ccgcgttgta
     3241 ggtgttgacg aagctctgca ccgcggtggc ggcgctcgag gtgttggccg acagcgtcag
     3301 cgacaccgtg gtgccgggcg ccgccttgac caggttgagc gtcacgccgt cgatggcgtt
     3361 gctcaccgtg ttgctggaac tgtagacgtc gccgccgtcg accttgatgt gcgcgtccac
     3421 cgcgccctgc gactgcgtgg tggtgaagaa gttgctgctg tcggcggtgg ccgaggacga
     3481 cacgctgacg gcgttggcgg taccggtgcc gcgcgaggtc agcaccagat gcgcgccgtt
     3541 gctgtccgac acgatcgagg cgttgacgcc ggtgttgtcg tcggcgctgt tgatggcatc
     3601 gcgcaggttg gccagcgtgt cggagcccgg cgcggtggtc acggaaaaac tcttgctgcc
     3661 gacggtgatc gtgatggtgc cgtcgcccac cgaggttgac gagttggcaa aggagccgga
     3721 cacctgcttg ctggccgtgg ccaggctcac cacttccacg tcgtagaacg cgctcagcgt
     3781 gccgctggcc gcggtggcgg tgacgatggt gttgtccgag gtattggcag cgatcttgcc
     3841 caggccgctg ctgccttcca gcgtctgcgc cgcggtctgc aacgcgctga tggcactctt
     3901 gaaggtgccc agcgccgaca gcttggcgtt gtccaccgcc tgtcccttgg tgatgcggtc
     3961 ggaatctggc gccttctcgg cagccaccag ctgcgacacc agcgagttga cgtcgagccc
     4021 cgagccgatg ccggccgagg tgatggtacc tgtaatggga ctggccatga tcgtgacccc
     4081 tgctgcaatt cctaggccac ggcctcgacc aggttggcgc cgctgcgctg ctcggcgagc
     4141 acgcgcgcga tgcgcagcgc ctcctcgctc gggatctgcc gcagcacggt gccgtccttc
     4201 gaatcgacca cggcgaccac gatgacgttg agatccttct ccaccgagaa cctcaggtcg
     4261 gtgcggatac tggcgaaatg acggttgagt tcgtccaccg ccttctgcag cgccgcattg
     4321 tccggcgccg gtgcagcgcc agcgccctgt gcatcggcat tggtggcctg cgtttttgtc
     4381 gtgggcaacg acaaatcgcg cggggaggca tccccacgct tcgtgctcgt cgtcgccgcg
     4441 gcgagcaccg gggtcatttc actgacgggc gtgctcatgt gttttctctc cggttgctgc
     4501 atctgaaaaa tccccgggat cggacaccgg ccgtccgacc ccggggctac cacctacctg
     4561 cttacttcag gagagacagc acgttctgcg gcgcggcgtt ggcctgcgcg agcatcgcgg
     4621 tgccggcctg ctgcaggatc tggctgcggg tcatcgccgc cgtttccttg gcgaagtccg
     4681 cgtccatgat gcggctgcgt gcggccgaca ggttctccga agccgtcgac aggctgttga
     4741 ccaccgactg gaagcggttc tgcagggcgc ccaggttgcc gcgggcggtg gacacggcgg
     4801 ccagcgccgc gtccatctgc ttgatcgccg tgtcggcgcc ggccgaggtc gtcagcgaca
     4861 gcgtcgagaa gccggtgttg gtggccgtcg ccgtggtcgc cgcggtcagg cccgtagtgg
     4921 ccgtggtgcc ggtgccggac agcgcgatgg tgatgcccgt gccggaggtg ctggtcagct
     4981 gcaccgtggt ggccgtgtcg ttcaccgcgt acacgccggt ctggtcagcc accgagttga
     5041 tggcatcgcg gatctggccc gcgcgcaccg tggcgctggc tgcggcgccg acgccgccga
     5101 cgctgatggc gccgttgccg ttgccgccgt cgatggtcag gtcgcccgcg gtgatcgcgg
     5161 tcagcgtggt cgcggcgacg ccggtcaccg tggcgctggt gtagctgccc aggttgaccg
     5221 aggtggcatc gacgatcgac gacaccgcga tggtctggcc ggcgttggcg ccgacctgga
     5281 aggcctggtt gctgaacgaa ccgtcgagca ggttgacgcc gttgaactgg gtctgctgcg
     5341 ccacgcgatc cacttcagcc ttgagctgct gcgcttcgct gttgagagcg gcgcggtcgg
     5401 tcgagctgtt ggaaccgttg cgcgactgca ccgccagttc gcggatgcgc tgcaggttgt
     5461 tggtcacttc ggccagcgcg ccttcggcgg tctgcgtcag cgagatgccg tcgttggcgt
     5521 tgcgcgaagc ctggtcgaga ccgttgatct gcgcggtgaa gcgatcggag atcgcgaggc
     5581 cggccgcgtc gtccttggcg ctgttgatgc gcaggccgga cgacaaccgc tgcatggcgg
     5641 tggccaggga catttgcgat gagttcaagt tgcgctgggc attgagcgac atcacattgg
     5701 tgttgatgac tgaagccatg atctactcct aaagtttggt gagggtcctg gccggcaatc
     5761 ccgaccttcg gattcgccag cttcaaaccc ctttgaagga tttgccgctg ctggtggatg
     5821 tagcgggcgg atttcgcaaa acttgatcgg acttttttgt cagccgacga acggtcatgt
     5881 cgctggcagt gtgtggaatg gaaaacgctt gcatcgtttt cggcacggcg cggcggatct
     5941 gaagaccgct cgggaatggc cgcgccgccg tgcgcatcag cgcagataat cgaacagcga
     6001 gaggccctgc accttcaggt acgactgctg cgcggcctgc agcagcgtct gcgccagcgc
     6061 cagccggctg gccgcgtcgg tcacgtcgac atcgcgcagg tccgacaggc tcttggccgt
     6121 gctggtgctc tgctgcgcca ggctgctggt ggcgtcgtcg agcatggcca gccgcgcgcc
     6181 gacaccggcg cgcatgtcgg agatgtgatc gcccgcggtc tgcagcgcat cgaggctggc
     6241 gctgtattgc gcctgcgtgg cgccgcccgg cgtctgcacc agcgcgatca ggttcttcac
     6301 cgtggcgaac aggtcgccgc tgccggtgtt ctggaacacc gcgctgccgg tgtcgccgtc
     6361 ggccaccgcg cggttatgcc cgatgtcgag caggcgctgc gtctggtcgc cgctgtagct
     6421 cacgccggaa gcgccgtcgg tgaacggcgg ctgcgcatcg ttgctgccgc cgaacaggta
     6481 gcggccggtg ccatcgtcgg aattggcgta gccgagcagc tgctggtaga gctgcgtcat
     6541 ctgcgtggcg agggccgagc gcgaggcatc gctctgcgtg gcgctgttgg cctggatggc
     6601 cagcgagcgc agctgcgtga gcacgtcatt ggtgccggac agcgcggttt cctcgaggcc
     6661 cagccggtcc tgcgcggtgc ccacgttggt ctgccagcgc gtgatgtcgg cggcggtctg
     6721 gtccagcacc agcgcgcgcg cggcgccggc cggattgtcc gacgcggtaa tgatcttctt
     6781 gccggtcgac agctcgttct gcacgtcggc cagcaccgtc tgctgcttga ggatgttgtc
     6841 gacgccctgc tggtgcaggc ccgcggtgga aatgcgcatg tgtgcctctt agccctgcgc
     6901 cgcctgcagc agcgactgga acacggtgtc ggcgacctgg atgatgcgcg ccgccgcctg
     6961 gtaggcctgc tggaagcgca ccaggtcggc cgcctcctcg tcgagattca cgccggacac
     7021 gctgtcgcgc tgcttctgcg tgtcgcccag gatcgcgttc tgcgcattgg cgcgcagcga
     7081 tgcctgctgc gcggtgctgc cggtggacga caccagcgcg gcgttggcgc tggtcaccga
     7141 ggtgagcccg ccgtcgagga cgcccgccga cgacagcgcc gacatggcgc gcgcgttgcg
     7201 gttgtcgccg ctgttggcgc cgttggccga aatgctgaag gtatcgcctg tcgcgggcgc
     7261 gcccttgatc tgcacgcgcc agccgttgaa gtcgatgttg ccgccagcac tgtaggtgta
     7321 gctgccgctg ccgttgatgc tgtaggtggt cgcggaagtg aaagcgatgg tgacgccgga
     7381 catcagcgcg gcgttgccgg cgtcgagcac ggtcggtgcg ctgatggtgg cggtgccggt
     7441 gttgccggtg gccgcgctgg ccgacaccgg tgccgccgcc gcgatcgcgc tggtgtcggt
     7501 ggtggcgagc tggacctgtg acgctgcctg gcgcgccggc tcgaccagga aacggtcgcc
     7561 ggccgccgga gtgcccgcga gcgtgatcga gatgccggcg ccggtgagcg gatcggccgc
     7621 ggtgccgctg cccgagagcg cgaccgctgc gccggtgctg gcgttggtca tgctccagct
     7681 gctgccgtcg tacttgagct ggtaatcgtc gccggtgatg gcgccggcat cggcgatgcc
     7741 gacctgtgcc gtcgcattgc cggtgttggc gctggcctgc agcgcggtgc cggtgatggg
     7801 cgcaaacaga tcgccgccaa gctggccgcg caggtcgatg ccggcgtggt tctggtcgtt
     7861 cactgccgag gtgatggcga tggccatgcg gccgagctgt tcctcggccg ggtcgagcac
     7921 ctcgcgccgc gcgtcgagca gcccgccgat tacgccgccg gaaacctgcg cggtgatgtc
     7981 gccgccgctg gtcagggcca gctgcaggcg gccgctgtga tagtcgtctt cggtgagcgt
     8041 cagcgaggtg gagcggct
//
LOCUS       DASZZC010000411         6367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_674607,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000411 DASZZC010000000
VERSION     DASZZC010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674607"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VHE37_08725"
     CDS             <1..760
                     /locus_tag="VHE37_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35655.1"
                     /translation="RDEDSKQELTVRICPEKDSVCGDYERNWCATCPKRPAAAQQVVG
                     GLTFEAFRRANKARCVKWHPAGIASWSPSDWLTAVTGELGELASLLKMRNRERDGLPG
                     NKFSPTQKQIADELADVLTYLDLLAEVLGIDLGQAAVSKFNEVSERVGFPDRLVVAHI
                     PPPVSSVPVAELEALRAANAEMLAILVNVEDDHYYRWGDGESESDAIAHALRAIIAKH
                     QPAQPEDNSRVLCACGWPPSQCRCEPAQPQGGGA"
     gene            757..2541
                     /locus_tag="VHE37_08730"
     CDS             757..2541
                     /locus_tag="VHE37_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HVT35656.1"
                     /translation="MSHIVFKAGHLFCGLGGGARGFKRGRARIGRMRASFRNVGGIDI
                     DPAAIADFERLAGCRGTVMDLASREQYIRFNGRQPPEGWREATPADIRRAMNNEAPNV
                     WFTSSPCKGLSGLLSEEKSKTDKYQALNELTLRGIWLALEAFADDPAEFILFENVPRI
                     QNRGRHLLDQIMQLLHAYGYAAAETTHDCGELGALAQSRKRFLLVARHMAKVPQFLYQ
                     PARNPLRAVGQVLGKLPLPGEGVGLGMHRLPQLQWRTWVRLAFVQAGSDWRSLNRLAV
                     EDGFLRDYAIAPAYHRAYGVNAWNEHAGTVTGNGRPASGAFNVADPRFDPKRYECNQY
                     GVIPWNEHSGSLINVKSPGQGTFAIADPRYDGKRGKRFNNVYRVVRFDQHAQTVTAGN
                     SPTSGGLSVADPRPGWTQRRGNNLVVTDWNEHSGVVIAGGKGVQGGWLSVADPRCGER
                     NWRRGPYGVIPWDKTAAAVAGAADVHNGFNTVADPRLPALDERLSCLIVAEDDTWHRP
                     FTTLELAALQSLFDLEEYEDFQLHGTSDSDHRERIGNAVPSAAAEAIANVMAHTLLLV
                     RSGNTDLIESSPIWVRPIVAAIQCGTSI"
     gene            2557..3021
                     /locus_tag="VHE37_08735"
     CDS             2557..3021
                     /locus_tag="VHE37_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HVT35657.1"
                     /translation="MIIDIRKPRVIHLTPEKNHLPAGSAEQAHSILLPDFGLVFPNIN
                     QIKSKNWKDCVKRGNDYELHQLQCATPEASELALLIDRTKSPCFDPQVSPRISTDDWY
                     WTATVDPSSSDFAYVVYFLSGYVSWNFQSHGGFVLPVLRVARGQYSAFGIEG"
     gene            complement(3093..3833)
                     /locus_tag="VHE37_08740"
     CDS             complement(3093..3833)
                     /locus_tag="VHE37_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35658.1"
                     /translation="MHRKIWKWMLHIAGGNKLLAIGLTVGGIVGLQIIGKYEVHAWQN
                     GLISPGEWWWLLDTEKAAAWAQAVFGVIGLATAIWVPFRLQQIDREKERLEEFKRRLF
                     AERIIYSPLVMMAEWMSSASKQIGALSNKVTAESGDDLKKAAGILHIEAVEQLSDKAL
                     WLSIEDCEEFLKIIGLAQAYRDMIDSAFRPHSEGYYFFMMNDDARRNAKTFAAIIGER
                     AGDLAAKFAPPIPQHEKDGISNHPVAPH"
     gene            3863..4045
                     /locus_tag="VHE37_08745"
     CDS             3863..4045
                     /locus_tag="VHE37_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35659.1"
                     /translation="MTFGVILWQGRALVYRPVPETRGWRANFQGEAMPIEITPELLQA
                     IEADLAKQRAKDRGEA"
     gene            4048..4287
                     /locus_tag="VHE37_08750"
     CDS             4048..4287
                     /locus_tag="VHE37_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35660.1"
                     /translation="MVPANKLPNMRRRVNRARNFPYNTCPASRHLHAMAEALATRGEY
                     SMLTEEPDRCAGTMLSVLESLFKARTELAMLKRPK"
     gene            4293..4493
                     /locus_tag="VHE37_08755"
     CDS             4293..4493
                     /locus_tag="VHE37_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HVT35661.1"
                     /translation="MESAIVQEEELLKSTGFEKRGWLENWLREQGIEYFRGKDGRVFT
                     TVGLLEAAKQRGARGSSQSIRF"
     gene            4493..5524
                     /locus_tag="VHE37_08760"
     CDS             4493..5524
                     /locus_tag="VHE37_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35662.1"
                     /translation="MARPRKKKTEGLPPRVYLAKGRYFIRQPDGSETKLAPSTASKAE
                     VWRAFLKVPQAKMSTGIYTLRALTMEYMRSEKFRGLAYGTQRDATRALNSVLAFPTAD
                     GREFGDCRAEDVTVGVMRLFMDKRGEGSKHRANLELAYTSAMYSWAFEREMVSRNPCK
                     GIKRFKVEARERYVEDGEYQERHALAGELGRPDVQVMMELAYLCRLRENEILKIMDSP
                     AFISPSAGLLAKRGKGSKTQWIAWSPRLETAIASARSIERRIPTPMLICSPHSRQPLS
                     LDAFQSAWQTIKQEARKRGHAVDWHFHDLKAKGVSDFEGDKHIASGHKTHRMTEVYDR
                     RKATVPSTR"
     gene            complement(5807..>6367)
                     /locus_tag="VHE37_08765"
     CDS             complement(5807..>6367)
                     /locus_tag="VHE37_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35663.1"
                     /translation="QRLTTLLALSSTSGPAIPSVLLLLFGLVGIVVVLVLWFELVLRN
                     AVFCVAVAAAPISAQSAPFDANPIPVMIQTGDHDGPVAPIPFVNPAVVRPVYEKLTQD
                     RAFIVAEASSHAEWTNYPLLPTATWGLPLAGKYSLAWMDYQLRHDASAAVTLTTADPH
                     LSYLWDSEVRLGGATTVMRGDGPVQP"
BASE COUNT         1272 a         2037 c         1935 g         1123 t
ORIGIN      
        1 acgcgatgaa gactccaagc aggaactgac tgttcgcatt tgtcctgaaa aggattcggt
       61 ctgtggtgat tacgagcgga actggtgcgc gacgtgtccc aagcgacccg ccgcagcgca
      121 gcaggtggtg ggtgggctga cgttcgaagc gttccgtcgg gccaataagg ctcgctgtgt
      181 taaatggcat cccgcaggaa tcgcatcgtg gtcaccatcc gattggctga cggccgttac
      241 gggcgagctt ggtgaactcg cgtccttgct aaagatgcga aaccgcgaac gcgatggctt
      301 gccgggcaat aagttttcgc cgacacaaaa acagattgcc gatgaattgg cagatgtgct
      361 gacttacctt gatctgctcg cggaagttct cggcattgat ctcggtcagg ccgcagtgtc
      421 caagttcaat gaagtgtcgg agcgcgtcgg ttttcctgat cgtttagtgg tcgcacatat
      481 cccgccgcct gtgtcgtcgg tgccggttgc ggagctggag gcgctgcgag ccgctaatgc
      541 tgaaatgctg gcgatacttg tgaacgtcga ggacgaccac tactacagat ggggcgacgg
      601 tgagagcgag tcggacgcta ttgctcacgc actgcgcgcc atcatcgcca agcaccaacc
      661 cgcgcagcca gaagataact cgcgcgttct gtgcgcctgt ggctggcctc cgtctcaatg
      721 ccgttgcgaa cccgcgcagc cacaaggcgg cggcgcatga gccacatcgt cttcaaagcc
      781 ggtcacctgt tctgcggcct gggcggcggc gcgcgcggct tcaagcgcgg ccgcgcccgc
      841 atcggccgca tgcgcgcgtc ctttcgcaac gtgggcggca tcgacatcga tcccgccgcc
      901 attgccgact ttgagcggct ggccggctgc cgcggcacgg tgatggacct ggcgtcgcgc
      961 gagcaataca tccgcttcaa cggccgccag ccgccggaag gctggcgcga ggccacgccg
     1021 gcagacatcc gccgcgccat gaacaacgag gcgcccaacg tctggttcac cagctcgccc
     1081 tgcaaggggc tgagcggcct gctcagcgaa gagaagagca agacggacaa gtaccaggcg
     1141 ctaaacgagc tcacgctgcg cggcatctgg ctggcgctgg aagccttcgc cgatgacccg
     1201 gccgagttca tcctgttcga gaacgtgccg cgcatccaga accgcgggcg ccacctgctc
     1261 gaccagatca tgcagctgct gcacgcctac ggctacgccg cggccgagac cacgcatgac
     1321 tgcggcgagc tgggcgcgct ggcgcagagc cgcaagcgct tcttgctggt ggcgcggcac
     1381 atggccaagg tgccgcagtt cctctaccag ccggcacgca atccgctgcg cgccgtgggc
     1441 caggtgctgg gcaagctgcc gttaccaggc gaaggcgtgg gcctgggcat gcaccggctg
     1501 ccgcagctgc agtggcgcac gtgggtacgc ctggcattcg tgcaggctgg atctgactgg
     1561 cgctcgctca atcgcctggc ggtggaggat ggcttcctgc gcgactacgc catcgccccg
     1621 gcctatcacc gcgcctatgg cgtcaacgcc tggaacgagc acgccggcac cgtgacgggc
     1681 aatggtcggc cggccagcgg cgcattcaat gtcgccgatc ctcgcttcga ccccaagcgc
     1741 tacgagtgca atcaatacgg cgtcatcccg tggaacgagc acagcggctc gctgatcaac
     1801 gtgaagtcgc ctggccaggg cactttcgcc atcgcagatc cacgctatga cggcaagcgc
     1861 ggcaagcgct tcaacaacgt gtatcgcgtg gtgcgcttcg atcagcatgc gcagaccgtc
     1921 accgccggca acagtcccac cagcggcggc ctgtcagttg cagatccgcg cccgggctgg
     1981 acgcagcgcc gcggcaacaa cctggtcgtc accgactgga atgagcacag cggcgtggtc
     2041 atcgccggcg gcaagggcgt acagggcggc tggctttcgg tggcggatcc gcgttgcggc
     2101 gagcgcaact ggcgccgcgg tccctatggc gtcattccat gggacaagac agctgctgct
     2161 gtcgccggcg cagctgacgt gcataacggg ttcaacacgg tggccgaccc tcgcctgccg
     2221 gccctggacg agcgcctgtc ctgcctgatc gtcgccgagg acgacacctg gcaccgcccc
     2281 ttcaccacgc ttgagctcgc tgcgctgcag agcctgttcg acctggaaga gtacgaagac
     2341 ttccagctgc acggcaccag cgacagcgac caccgcgagc gcatcggcaa cgccgtgccc
     2401 agcgccgcgg ccgaggccat cgccaacgtc atggcgcaca cgctgctgct ggtgcgcagc
     2461 ggcaataccg acctgattga gtcgagcccc atctgggttc gtcccatcgt cgccgccatt
     2521 cagtgcggca cgtccattta acccggggag tcacgcatga tcatcgacat ccgcaagcct
     2581 cgcgtcatcc acctcacccc ggaaaagaac catctgccgg ccggctcggc cgagcaggcg
     2641 cactccatcc tcctgccaga cttcggcctg gtgttcccca acatcaacca gatcaaaagc
     2701 aagaactgga aggattgcgt caagcgcggc aacgactacg aactgcacca gctgcagtgt
     2761 gcgacgcccg aggccagcga gctcgcgctg ctgatcgacc gcaccaagtc gccgtgcttc
     2821 gatccgcagg tgtcgcccag gatcagcacc gacgactggt actggacggc caccgtcgat
     2881 ccgtcgtctt cggacttcgc ctacgtcgtc tatttcctca gcggctacgt cagctggaac
     2941 ttccagagcc acggcgggtt tgtgctcccc gtcctgcgcg tcgcccgcgg tcagtactcg
     3001 gcttttggca ttgaggggta agccatgaca tcgcttcacc tgttctccgg ccttgtcggc
     3061 gagagctgca tccacgactc gctgaacctg attcagtgcg gagctacagg atgattgctt
     3121 atgccatctt tttcatgctg cgggatcggc ggagcaaact tcgctgccag atcacctgcg
     3181 cgctcgccaa tgattgcagc gaaggttttg gcattgcgtc tggcatcgtc attcatcatg
     3241 aagaagtaat acccttcgct gtgcggccga aaggccgaat caatcatgtc cctatacgcc
     3301 tgagcgaggc caatgatttt aagaaattcc tcgcaatcct caatcgacag ccagagggct
     3361 ttatcgctga gctgctcaac cgcttcgatg tgcaatatcc ccgctgcttt tttcaggtca
     3421 tcgcccgatt cggcggtgac cttgttactc agtgcaccga tctgtttgct tgccgacgac
     3481 atccattcgg ccatcattac caagggggaa tagataattc gctcggcgaa gagtcggcgt
     3541 ttgaattctt ccaggcgttc cttttcacga tcgatctgct gcagccgaaa tggcacccaa
     3601 atagcagtag caagtccgat gacaccaaag accgcctgag cccacgcggc ggctttttcc
     3661 gtgtccagca gccaccacca ttccccaggc gaaatgagtc cgttctgcca agcatggact
     3721 tcatacttgc cgatgatctg cagaccaacg atgccgccta cagtcaatcc aattgcaagg
     3781 agcttatttc cgccggcaat gtgaagcatc catttccata tttttcgatg catggccgtg
     3841 ccttcgcgct ggagaacgca gcatgacctt cggcgtcatc ctctggcaag gccgcgcact
     3901 ggtgtatcgc ccagtgccgg aaacgcgcgg ctggcgcgcc aactttcagg gcgaagccat
     3961 gccgatcgag atcacgcccg agctgctgca ggccatcgag gcggatctgg ccaaacagcg
     4021 cgccaaggac cgcggcgagg cctgaccatg gtccccgcca acaaactgcc gaacatgcgc
     4081 cgccgggtaa atcgggcgcg caattttcca tacaacacct gcccggcgtc gcggcacctg
     4141 catgccatgg ccgaggcgct ggctacgcgc ggcgaatatt cgatgctcac cgaggaacca
     4201 gaccgctgcg ccggcacaat gctcagcgtg ctggagtcgc tgttcaaagc gcgcaccgag
     4261 ctggccatgc tcaagcggcc gaagtgatgc gcatggaatc agccatcgtc caggaagaag
     4321 agctgctcaa gtccaccggc ttcgaaaagc gcggctggct ggaaaactgg ctgcgcgagc
     4381 agggcattga atacttccgc ggaaaagacg gccgtgtgtt caccacggta ggattgctgg
     4441 aggccgccaa acagcgcggc gcgaggggat cgagccagtc gatccgattc tgatggccag
     4501 accacgcaag aagaaaaccg agggcctgcc gccgcgggta tacctggcga agggccgtta
     4561 tttcatccgc cagccggacg gcagcgagac aaagctggcg ccatcaacgg ccagcaaggc
     4621 ggaggtgtgg cgcgccttcc tcaaggtgcc acaggccaag atgtccaccg gcatttacac
     4681 gctgcgcgcg ctgacgatgg agtacatgcg atcggagaaa ttccgcggtc ttgcctatgg
     4741 cacccagcgc gatgccacgc gagcgctcaa cagcgtgctc gccttcccca ccgccgacgg
     4801 ccgcgagttc ggcgactgtc gggccgagga cgtgaccgtc ggcgtgatgc gcctgttcat
     4861 ggataagcgc ggcgaaggca gcaagcaccg ggcaaaccta gagctggcat atacgtcggc
     4921 gatgtactcc tgggcattcg agcgtgagat ggtgagccgc aacccatgca agggaatcaa
     4981 gcggttcaag gtcgaggcgc gggagcggta tgttgaggat ggcgaatacc aggaacgcca
     5041 tgcgctcgca ggcgagctcg gccgccccga cgtgcaggtg atgatggaac tggcgtatct
     5101 gtgtcgcctg cgcgagaacg agatcttgaa aatcatggac tcgccggcgt tcatctcccc
     5161 gtcggccggc ctgctcgcca agcgcggcaa gggcagcaag acgcaatgga ttgcatggtc
     5221 accccgactg gagaccgcca tcgcctcggc tcggtccatc gagcgacgca tccctacccc
     5281 catgctcatc tgctcgcctc attcgcgcca gcctctatcc ctggacgcat tccaaagcgc
     5341 gtggcagacc atcaagcaag aggccagaaa acgcggccac gcggtcgact ggcacttcca
     5401 tgacctgaag gcaaaagggg tcagcgattt cgagggcgac aagcacatcg ccagcggcca
     5461 caaaacgcac cggatgaccg aggtctatga ccgcaggaaa gcgacagtcc cgtcaacccg
     5521 ataacccatg ggttttggga atttcgtcag agttttggga attagcgcaa aaaatccatt
     5581 taaaatcaaa gatcataaag ggattttaag tcccttgcgg ctaccagtta cgccactcgg
     5641 gccgcgtgcg cgcagcatag ccgaggcgtt gcgcaaacac cgcgcggcga tttttaccct
     5701 ttaccgcacc ggccagtggc ttgttactaa ttatagtatt tatttttaaa cgataaatat
     5761 tttataacaa gaaaaacggc cgtgcggtat gaccctttgc acgccgctac ggctgcaccg
     5821 gcccatcgcc gcgcatcacc gtggtggcgc cgcccaggcg cacttcgcta tcccacaggt
     5881 acgacaggtg cggatcggcg gtggtgagcg tgacggctgc ggacgcatcg tggcgcaact
     5941 ggtagtccat ccacgccagc gaatattttc cggcgagcgg caggccccag gtcgccgtcg
     6001 gcagcagcgg gtagttggtc cactcggcat gcgacgacgc ttcggcgacg atgaacgcac
     6061 gatcctgcgt gagcttctcg tacaccggcc gcaccaccgc gggattgacg aacgggatcg
     6121 gcgcaaccgg cccgtcgtga tcgccggtct ggatcatcac cgggatggga ttggcatcga
     6181 acggcgctga ctgcgcgctg atcggcgccg ccgcgaccgc gacgcagaac acggcgttgc
     6241 gcagcaccag ctcgaaccac aggacgagca cgacgacgat cccgaccagc ccgaacagca
     6301 gcagaagcac cgacgggatc gccgggcccg aggtcgaact cagtgcgagc agcgtggtca
     6361 gccgctg
//
LOCUS       DASZZC010000412         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_939631,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000412 DASZZC010000000
VERSION     DASZZC010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939631"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..>1657
                     /locus_tag="VHE37_08770"
     CDS             165..>1657
                     /locus_tag="VHE37_08770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF044950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT35664.1"
                     /translation="MNSSSALLSGEQEQDSTAAGPRPARNALVIDDDEFVCGAIAQQL
                     RELGVGQVITSSGGNEAVRLLNQGGPFDLIVTDLAMPSYDGLQLTRLIAVRQPRAAVL
                     YMSAAGPKMLAAAKSLAAGRGLRVLGSLSKPVRIAELRNAVLGQHKPDAAPPAEAGLP
                     ADHAPLVRMIDRLMPLQPLQKELGAVRAQALGKVQPAELAQAADQLGSLVHRHCVQLE
                     AERAGVVRLLDQMTFGLDEMSGYLSNEAASRREALDESRALSSRLLGEVTDLDADIQS
                     ADDLSALQEDVRTRLDRINASLQEAKQREDARLQEFEQRAAQMNARIDHLESETRTLR
                     ESVAREHQAACTDRLTGVPNRLAYDQRVRQDHASWKRYGQPMIIAVWDIDRFKKVNDT
                     YGHACGDKALAAVGLLLSKHIRETDFVARYGGEEFAMILHGLDAEAAHQLTDKLRATI
                     EKIDFRYNQQPVPLTVSCGYASFSGDDTPESVFERADAALYRAKNEGR"
BASE COUNT          288 a          584 c          554 g          231 t
ORIGIN      
        1 cattttttat attattatat ttttaataac atcgcagaat tgcttgcggt cacagcctgc
       61 cggcattttt ttctgcgcgc taaagattcc gcgccgcccg ccgatagcag gatcgccggg
      121 ccccggtccg gcccccgttg ctggcattgc aggacatccg aacgatgaac agctcaagcg
      181 cgctgttgtc aggcgaacaa gaacaagatt ccaccgccgc cgggccgcgt cccgcgcgca
      241 acgcgttggt gatcgacgac gacgagttcg tctgcggcgc catcgcccag cagttgcgcg
      301 aactgggcgt cggacaggtc atcaccagct ccggcggcaa cgaggcggtg cgcctgctca
      361 accagggcgg gccgttcgat ctgatcgtca ccgacctggc catgcccagc tacgacggcc
      421 tgcagctcac gcgcctgatc gccgtgcgcc agccgcgcgc cgcggtgctg tacatgagtg
      481 cggccgggcc caagatgctg gccgccgcca agtcgctggc cgccgggcgc ggcctgcgcg
      541 tgctcggctc gctgagcaag ccggtgcgca tcgccgagct gcgcaacgcc gtgctgggcc
      601 agcacaagcc ggacgccgcg ccgccggccg aggccggcct gccggcggac cacgcgccgc
      661 tggtgcgcat gatcgaccgc ctgatgccgc tgcagccgct gcagaaggaa ctgggcgcgg
      721 tgcgcgcgca ggcgctgggc aaggtgcagc cggcggaact ggcgcaggcc gccgaccagc
      781 tgggctcgct ggtgcaccgc cactgcgtgc agctggaagc cgagcgcgcc ggcgtggtgc
      841 ggctgctcga ccagatgacc ttcggcctcg acgagatgtc cggctatctc agcaacgagg
      901 cggcgtcgcg gcgcgaagcg ctggacgaga gccgcgcgct gagcagccgc ctgctgggcg
      961 aggtcaccga tctcgacgcc gacatccagt ccgccgacga tctcagcgcg ctgcaggaag
     1021 acgtgcgcac gcggctggac cgcatcaacg cctcgctgca ggaagccaag cagcgcgagg
     1081 atgcgcgcct gcaggaattc gagcagcgcg cggcgcagat gaacgcgcgc atcgatcacc
     1141 tggaatcgga aacgcgcacg ctgcgcgagt cggtggcgcg cgagcaccag gccgcctgca
     1201 ccgaccgcct caccggcgtg cccaaccgcc tggcctacga ccagcgcgtg cgccaggacc
     1261 acgcctcgtg gaagcgctac ggccagccga tgatcatcgc ggtgtgggac atcgaccgct
     1321 tcaagaaagt gaacgacacc tacggccatg cctgcggtga caaggcgctg gccgcggtcg
     1381 gcctgctgct gtccaagcac atccgcgaaa ccgatttcgt cgcgcgctac ggcggcgagg
     1441 aattcgccat gatcctgcac ggactcgatg ccgaggccgc gcaccagctc accgacaagc
     1501 tgcgcgcgac gatcgagaag atcgacttcc gctacaacca gcagccggtg ccgctcaccg
     1561 tgtcctgcgg ctacgccagc ttcagcggcg acgacacgcc ggagagcgtg ttcgagcgcg
     1621 ccgacgccgc gctgtaccgc gccaagaacg aaggccg
//
LOCUS       DASZZC010000413         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_290191,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000413 DASZZC010000000
VERSION     DASZZC010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290191"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VHE37_08775"
     CDS             complement(<1..638)
                     /locus_tag="VHE37_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT35665.1"
                     /translation="MSNGNRQYIEHAADVERQLRKAGRKPASNSRAVTEFILRVQAQL
                     RSPLPRGARVLDFGCAAGATVTTLTDLGYDASGVDILEYWGKDRGLIGEEFNEPSDEI
                     RRRLFAVDPDDGRLPFADGTFDLIISDQVLEHVFDLPPVFAEHVRILKPGCVAIHRFP
                     KPTGLIEPHTKLPFTSLHRFDSYLALCAIAGVRNERQHGMGWRDTYQSNRNL"
     gene            complement(677..1471)
                     /locus_tag="VHE37_08780"
     CDS             complement(677..1471)
                     /locus_tag="VHE37_08780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT35666.1"
                     /translation="MAADLAGKCALVTGASSGLGVDFARELAARGARLVIVARRDELL
                     KLVQEEIKNRYGVEVMTVALDLGAPSAPQELYDLLRQQNVQVDVLVNNAGFGLFGMEL
                     DIPWERTRDMLQLDIVALTHLTKLFARDMVKRGSGWLLQVASIGAYQPSPTYAAYSAA
                     KSYVLSFSDALNLELRGTGVSCTALSPGYTETAFLEVSGQKPTLFHQLTAMKPAEVAR
                     IGVQRMLDRGYSVVPGWINKLLAFSTRLTPRPLLAWLTWRLMRNPS"
     gene            1606..2550
                     /locus_tag="VHE37_08785"
     CDS             1606..2550
                     /locus_tag="VHE37_08785"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVT35667.1"
                     /translation="MASATRSTWIVSGLLLALSAGCASAGGNDVGPGETRYEMKFGGR
                     DRSYVVHVPSSGARANSALVISLHGGGGNAHTNAQQTHFNDESDREGFIVVHANGTGE
                     SRPFLNAMGKGFLFTWNAGTCCGYARDQGVDDVGFIRAMVAAIRSKYSIDPKRIYATG
                     ISNGGMLSYRLACEASDLIAAIGVVSGAQAVANCQPSQGVSVMHIHGTADQNVLLAGG
                     IGPKGYDKVPKPPVMDAINFWVKFDGAAAQPKTTISGNLRTDYYHGTRGDVEFILITG
                     GGHSWPGGEQMLAALDPPSKDMAATPTIWKFFVAHPKP"
BASE COUNT          459 a          816 c          847 g          442 t
ORIGIN      
        1 aacaggttgc gattggattg ataagtgtcg cgccagccca tgccgtgctg ccgctcgttg
       61 cgcacgccgg cgatcgcgca gagcgcgagg taggaatcga aacggtgcag cgaggtaaac
      121 ggcagcttgg tgtggggctc gatcaggccg gtgggtttgg gaaagcggtg aatcgccacg
      181 cagcccggct tgaggatgcg cacatgctcg gcgaataccg gcggcaggtc gaacacgtgt
      241 tcgagcacct ggtcggagat gatgagatcg aaggtgccgt cggcgaatgg caggcgtcca
      301 tcatctggat caacggcaaa caggcgtcgc cggatttcgt cgctcggctc gttgaattct
      361 tcgccgatca ggccgcggtc ctttccccag tactccagga tgtcgacgcc gctggcgtcg
      421 tagccaaggt cggtcagggt ggtcacggtg gcgccggcgg cgcagccgaa atcgagcacg
      481 cgcgcgccgc gcggcagggg agaacgcagt tgcgcctgga cgcgcaggat gaattcggtg
      541 accgctcgcg aattggaggc gggtttcctg cccgccttcc gcagctggcg ctccacgtcc
      601 gccgcgtgtt cgatgtactg ccggtttccg ttgctcatgc cgcgatttta gccttgaacc
      661 gccggcatcc aggcatctag gacggattcc tcatcagccg ccaggtgagc caggccagca
      721 gcggccgcgg cgtcaggcgc gtggaaaaag ccagcagctt gttgatccag cccggcacca
      781 cgctgtagcc gcggtcgagc atgcgctgca cgccgatacg cgccacctcg gcgggcttca
      841 tcgccgtcag ctggtggaac agggtcggct tctgcccgga gacctcgagg aaggcggttt
      901 cggtatagcc cggcgagagg gccgtgcacg atacgccagt gccgcgcagt tcgaggttca
      961 gcgcgtcgct gaacgaaagc acgtaggact tggccgcgga gtaagccgcg taggtgggcg
     1021 agggctggta ggcgccgatc gaggcgacct gcaggagcca tccgctgccg cgtttgacca
     1081 tgtcgcgtgc gaacagcttg gtcaggtggg tgagggcgac gatatccagc tgcagcatgt
     1141 cgcgcgtgcg ttcccagggt atgtccagtt ccatgccgaa caggccgaag ccggcgttgt
     1201 tcaccagcac gtcgacctgc acgttctgct gcctgagcag atcgtaaagt tcctgcggtg
     1261 ccgacggcgc cccgagatcc agtgccaccg tcatcacctc gacgccgtag cggttcttga
     1321 tttcttcctg caccagcttg agcagttcat cgcggcgcgc cacgatgacc aggcgcgcgc
     1381 cgcgcgcagc cagttcacgc gcgaaatcga cgcccagccc ggacgacgcg ccggtgacca
     1441 gcgcgcactt gcccgcgaga tccgctgcca tggaaattcc ccgattattt ttgcagcaaa
     1501 gtaaagtccg ccatccgccc cgtcaagaaa tgccgcaaaa cccttacact ccgtgcggat
     1561 tttccgcgcg tagcatgccg ttgccaaact ccaggagggc agatcatggc cagcgcaact
     1621 cgtagcacat ggatcgtttc ggggttattg ctggcattga gtgccggctg cgccagtgcc
     1681 ggcggcaatg acgtcggacc gggcgagacg cgttacgaga tgaagttcgg cggccgcgac
     1741 cgcagctacg tggtgcacgt gccgtcgagc ggcgcgcgcg cgaactccgc gctggtcatt
     1801 tcgctgcacg gcggcggagg caatgcgcac accaatgccc agcagacgca tttcaacgac
     1861 gaatcggacc gcgaggggtt catcgtcgtg catgccaacg gcaccggcga gtcgcggccg
     1921 ttcctcaacg ccatgggcaa gggcttcctg ttcacctgga atgccggcac ctgctgcggc
     1981 tatgcgcgcg accagggtgt cgatgacgtc ggcttcatcc gcgccatggt ggcggccatc
     2041 cgctcgaagt attccatcga tcccaagcgc atctatgcca ccggcatcag caacggcggc
     2101 atgctcagct atcgcctggc ctgcgaagcc agtgacttga tcgccgccat cggcgtggtg
     2161 tcgggcgcgc aggcggtggc caattgccag ccctcgcaag gcgtgtcggt gatgcacatc
     2221 catggcaccg ccgaccagaa cgtgctgctg gcgggcggta tcggccccaa gggctacgac
     2281 aaggtgccca agcccccggt gatggatgcg atcaatttct gggtcaagtt cgacggcgcg
     2341 gcagcgcagc ccaagaccac catcagcggc aacctgcgca ccgactacta ccacggcacg
     2401 cgcggcgatg tggagttcat cctcatcacc ggtggcgggc actcatggcc gggcggcgag
     2461 cagatgctgg cggcgctgga cccgccatcg aaggacatgg cagccacgcc cacgatctgg
     2521 aaattcttcg tcgcgcatcc caagccctga ctggcgcgct tcag
//
LOCUS       DASZZC010000414         6950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_735310,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000414 DASZZC010000000
VERSION     DASZZC010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735310"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..989)
                     /locus_tag="VHE37_08790"
     CDS             complement(96..989)
                     /locus_tag="VHE37_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVT35668.1"
                     /translation="MATHLPLRGIVTMLAAVGFLSLMDAGLKQLSPFYPPVEIAALRG
                     GASWPLALSWVFASGGARTLFKVRWRLHLLRGALAVMMIPTFVYALRVLPLTTAYVIF
                     FIAPMMITALSALILNERVSRGRWLAVGAGFAGVLVAMRPTGEGMFSLAALSVLAAAT
                     GYALAAITVRSLAQTDSKQALVFWVLTSMTFGSGVLAIGSWVPVAREHWWIVGAIGVS
                     GALGQYAITEAFRIAEASVIAPLEYSALGWGLGCDLLLWGVLPDAWTWAGAAIIVASG
                     VYLMRHERDQATAPVLPESAA"
     gene            1130..2137
                     /locus_tag="VHE37_08795"
     CDS             1130..2137
                     /locus_tag="VHE37_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HVT35669.1"
                     /translation="MAYDRRTFLRMGTGLAAMAATAAPRIGAAANRIDTATVKTGGVR
                     MVPVDGKYNVWTKRVGFSSTRMLTLHGGPGCTHEYLECFDDFLPQQGIEYYYYDQLGS
                     AYSDQPKDASLWTLDRFREEVEQVRAALGLKNFYLYGHSWGGMLAIEYALQYPQQLKG
                     LIISDMTASIDSYLAYINQLRKQLPPEVIATLEKYEAKNDFDAPEYQQAMFSEVYTRH
                     LCRLDPWPEPLQRTFRNMNQQVYNTMQGANEFQVTGNLRSWNRWDDLKRIEVPTLLLV
                     GRHDEMNPDDIVKMGQLMPKSRVHILENGSHMCMYDDQQMYFRHLTAFVRDVQAKRFS
                     T"
     gene            complement(2150..3763)
                     /locus_tag="VHE37_08800"
     CDS             complement(2150..3763)
                     /locus_tag="VHE37_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT35670.1"
                     /translation="MPYTHSHWLPDTSAPVHESTIGSVLAAAAADVPDATALVSWLPQ
                     HDQRRAWTYAQLQRDAQRTARALLTRFAPGERIAVWAHNIPEWLLLEFGAALAGLVLV
                     TVNPGYRPRELAYVLQQSRAAGLFHVREVRGNPMTQAVAQVRSQLPGLREVIAIEQFE
                     EFLARAPAAGALPEVKPGDAAQIQYTSGTTGFPKGALLHHRGITNNARLVMQRFQCRP
                     GDVFAHAMPFFHTGGCVCAALGSIQARATHGFLPGFDAGHLLELVERERATHVFGVPT
                     MLLAMMAHPGLATRNLSSLRASISGGASVPAELVREIEAKLGLKFSIIFGQTESSPVV
                     TMTRLDDTPEDKAATIGQPLPQTEVKIVDPASGEILPVGQTGELCTRGYLVMHGYNDN
                     PQATAAAIDADGWLHTGDLASMDARGYFRIRGRLKEMVIRGGENLFPVEIENVLHEHA
                     EVGDVAVVGVPDERMGEELAAFVRRAAGSAVTEETLRTHVRTQLAAQKTPRYWIFVDE
                     FPLTGSGKVQKFVLRERWERGEFAPGRNP"
     gene            3988..5736
                     /locus_tag="VHE37_08805"
     CDS             3988..5736
                     /locus_tag="VHE37_08805"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HVT35671.1"
                     /translation="MISRAAFALAAVLASAAASADPRPNFVVILIDDAAFTDLGAYGG
                     EAHTPNLDMLAARGAMFTSYHTSPLCSPSRAMLLTGIDNHKTGVATIPETLPSEQVGK
                     HGYSMFLEPGVDTVAQHLKGAGYRTYMSGKWHLGHGPGQLPDGHGFDHSLALDASGAD
                     NWQQKPYMPYYATADWFEDGKPVQLPENYYSSEMIVDHMIGYLKRDQANAAPFLAYVP
                     FQAVHIPVQAPREFTAHYAGVYDGGWDATRERRWQRAKELHLIPADAELPPAHDKLRR
                     WDAQSAEDKALFAKSMAVYAGMIEAMDANVGKLIAYLKETGQYDHTIFFVTSDNGPEP
                     SDPLAQVGFATWMSQNGYERKIDTLGEKGSMNFIGPEWANAAASPDRLFKFHSAEGGL
                     RVPLIVSGPGVSQGMVNSLSFVTDLTPTIYDYAGIDPATWNGKVPLTGKSLAPVLRGQ
                     AERTYAPDVPVGIEVGGNAALFKGDYKLTHISLPWGDAKWHLYHLTEDPGEVHDLSTL
                     DAERYKSMLADYDNYAKQMGVLKLPDDFNPHTQVAINALEKQVEYLRVKIAAAAVFII
                     AIVIWLVRRRRTAADS"
     gene            5912..6154
                     /locus_tag="VHE37_08810"
     CDS             5912..6154
                     /locus_tag="VHE37_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35672.1"
                     /translation="MRGLPQRALRLAAEYAERYALDHTGLREDDAFWQRLRAQFRDDE
                     IVDLALCCTLWLGAGRTMRVLDVGQTCALTLTRAAR"
     gene            complement(6179..6649)
                     /locus_tag="VHE37_08815"
     CDS             complement(6179..6649)
                     /locus_tag="VHE37_08815"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVT35673.1"
                     /translation="MQPASAARLRRALAVAALLLPGVAHAADGDYTGRWRTPPEPDGV
                     DSTVELYLQDDHLYGRVLQVRDRNGAEVLRRCERCDGARRGQPLAGMVFIENLHRDGE
                     RWIGGRVIDLRPGMTQGVTGTVEISLDHGRAVVYGYKGLHMLGESSTWTRADGD"
     gene            complement(6651..>6950)
                     /locus_tag="VHE37_08820"
     CDS             complement(6651..>6950)
                     /locus_tag="VHE37_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35674.1"
                     /translation="GSLRAYLSYNYVGYRIGTVITEQRRGLTSLPGYGVLSGRVAASG
                     LHVGDTGSLEVALWGRNLLNRDYVVFAVDNLPQADRAVLWGEPLTVGLDLVYRYF"
BASE COUNT         1225 a         2401 c         2297 g         1027 t
ORIGIN      
        1 attagagcac gcgccgcatg acgcgaatgt tggagggacg acgaaatggc aaagttccta
       61 cagcagtttg cgccgctgtc ggaagtgcgg aagcttcagg ccgccgactc gggcagcacg
      121 ggcgccgtcg cctgatcgcg ctcgtggcgc atgagataga cgccgctggc gacgatgatg
      181 gccgcgccgg cccaggtcca tgcgtcgggc agcacgcccc acagcagcag gtcgcagccc
      241 aggccccagc ccagcgcgga atattccagc ggcgcgatca ccgaggcctc ggcgatgcgg
      301 aacgcctcgg tgatggcgta ctggcccagc gcgccggaca cgccgatggc gccgacgatc
      361 caccagtgct cgcgcgccac cggcacccag ctgccgatgg ccagcacgcc cgagccgaag
      421 gtcatggagg tgagcaccca gaacaccagc gcctgcttgc tgtcggtctg cgccaggctg
      481 cgcacggtga tggcggccag cgcgtagccg gtcgccgccg ccagcaccga cagcgccgcc
      541 aggctgaaca tgccttcgcc ggtcggccgc atcgccacca gcacgccggc gaagcccgcg
      601 cccaccgcca gccagcggcc gcggctgacg cgctcattca ggatcagcgc cgacaatgcc
      661 gtgatcatca ttggcgcgat gaagaaaatg acataggccg tggtcagcgg cagcacgcgc
      721 agcgcgtaga cgaaggtggg aatcatcatc accgccagcg cgccgcgcag caggtgcagc
      781 cgccagcgca ccttgaacag cgtgcgtgcg ccgccgctgg cgaacaccca gctcagcgcc
      841 agcggccacg acgcgccgcc gcgcagcgcg gcgatctcga ccggcggata aaacggcgac
      901 agctgcttga ggccggcgtc catcagcgac aggaagccga cggcagccag catcgtcacg
      961 atgccgcgca atggaagatg ggtggccatg aggcgcgcga ggataacaaa gcgccctgtc
     1021 tgcacgccgc ttccgcgccg cttgcaccgc gcttgcgcag ctgcccgcta gattgcgcgg
     1081 caggagcagg agcatgatgc ggacacggcg caatctcggg gagacaagca tggcttacga
     1141 ccgcaggacc ttcctgcgca tgggcacggg cctggccgct atggcggcaa ccgccgcgcc
     1201 gcgcatcggc gcagccgcga accgcatcga caccgccacg gtgaagaccg gcggcgtgcg
     1261 catggtgccg gtcgacggca agtacaacgt gtggaccaag cgcgtgggtt tctcctccac
     1321 gcgcatgctc acgctgcacg gcgggccggg ctgcacgcac gaatacctgg aatgcttcga
     1381 tgatttcctg ccgcagcagg gcatcgagta ctactactac gaccagctcg gctcggccta
     1441 ttcggaccag ccgaaggacg cgtcgctgtg gacgctcgac cgcttccgcg aggaagtgga
     1501 gcaggtgcgc gcggcgctgg gcctgaagaa cttctatctc tacggccatt cgtggggcgg
     1561 catgctggcc atcgagtacg cgctccagta tccgcagcag ctcaagggcc tgatcatctc
     1621 cgacatgacg gcgagcatcg attcgtacct cgcctacatc aaccagctgc gcaagcagct
     1681 gccgccggaa gtgatcgcca cgctggagaa gtacgaggcg aagaacgact tcgacgcgcc
     1741 ggaataccag caggccatgt tcagcgaggt gtacacgcgc cacctgtgcc ggctcgaccc
     1801 gtggcccgag ccgctgcagc gaacgttccg caacatgaac cagcaggtct acaacaccat
     1861 gcagggcgcc aacgaattcc aggtgaccgg caacctgcgc agctggaacc gctgggatga
     1921 cctcaagcgc atcgaggtgc cgacccttct attggtcggg cgccacgacg agatgaatcc
     1981 cgacgacatc gtgaagatgg gccagctgat gcccaagtcg cgcgtgcaca tcctggagaa
     2041 cggcagccac atgtgcatgt acgacgacca gcagatgtac ttccgtcacc tcaccgcgtt
     2101 cgtgcgcgat gtgcaggcca agcgcttcag cacctgagcg cctggcagct catggattgc
     2161 gccccggcgc gaactcgccg cgctcccagc gctcgcgcag gacgaatttc tgcaccttgc
     2221 ccgagccggt gagcggaaac tcgtcgacga agatccagta gcgcggtgtc ttctgtgcgg
     2281 ccagctgcgt gcgcacgtgc gtacgcagcg tttcttcagt gaccgccgag ccggcggcgc
     2341 ggcgcacgaa ggcagcgagc tcttcaccca tgcgctcatc gggcacgccg accacggcga
     2401 cgtcgcccac ttcggcgtgc tcgtgcagca cgttttcgat ttcgaccgga aataaattct
     2461 cgccgccgcg gatgaccatc tccttgagcc ggccgcggat gcggaaatag ccgcgtgcgt
     2521 ccatcgaggc caggtcgccg gtgtgcagcc agccgtcggc gtcgatggcg gcggccgtgg
     2581 cctgcgggtt gtcgttgtag ccgtgcatca ccaggtagcc gcgcgtgcac agctcgccgg
     2641 tctggcccac cggcaggatc tcgccgctgg cgggatcgac gatcttcacc tcggtctgcg
     2701 gcagcggctg gccgatggtg gcggccttgt cctcgggcgt gtcgtccagc cgcgtcatgg
     2761 tgaccaccgg cgacgactcg gtctggccga agatgatgct gaacttcagg ccgagcttcg
     2821 cttcgatctc gcgcaccagc tccgccggca cgctggcgcc gccgctgatc gacgcgcgca
     2881 ggctggacag attgcgcgtg gccaggcccg gatgcgccat catcgccagc agcatggtcg
     2941 gcacgccgaa cacgtgggtg gcgcgctcgc gttcgaccag ttccagcagg tggccggcat
     3001 cgaagcccgg caggaagccg tgcgtggcgc gcgcctggat gctgcccagc gccgcgcaca
     3061 cgcagccgcc ggtgtggaag aacggcatgg cgtgcgcaaa cacgtcgccc ggccggcact
     3121 ggaagcgctg catcaccagc cgcgcattgt tggtgatgcc gcggtgatgc agcagcgcgc
     3181 ccttggggaa ccccgtggtg ccggaggtgt actggatctg cgcggcgtcg cccggcttca
     3241 cttccggcag cgcgccggcg gccggcgcgc gggccaggaa ctcttcgaac tgctcgatgg
     3301 cgatgacctc gcgcaagccg ggcagctggc tgcgcacctg cgcgaccgcc tgcgtcatcg
     3361 gattgccgcg cacctcgcgc acgtggaaca ggccggcggc gcgcgactgc tgcagcacgt
     3421 aggcgagctc gcgcgggcga tagccgggat tgaccgtgac cagcaccagc ccggccagcg
     3481 cggcgccgaa ttccagcagc agccattcgg gaatgttgtg cgcccacacc gcgatgcgct
     3541 cgccgggcgc aaagcgcgtg agcagtgcac gcgccgtgcg ctgcgcgtcg cgctgcagct
     3601 gcgcgtaggt ccacgcgcgg cgctggtcgt gctgcggcag ccacgacacc agcgcggtgg
     3661 catcgggcac gtcggccgcc gctgcggcca gcacgctgcc gatggtggat tcgtggaccg
     3721 gcgcgctggt gtcgggcagc cagtgcgagt gcgtataggg catggattat tcacgcatgg
     3781 cccatccgcg cgcgcaacac cgcggccgca cgcgccgcag acccggaaaa ggcctccacc
     3841 gcaccgcggt tttgtgttta ataattatcg tattaaaata aaataatatt attttttata
     3901 acaccaaaaa tatccgtgcg gtcggcatga aaaccgtggc attgtcacgc ccgcaaagac
     3961 cacaacatcg catcactggg gagagacatg atttcgagag ccgccttcgc gctcgccgcg
     4021 gtgctggcca gcgcggctgc cagcgccgac ccgcgtccca actttgtcgt catcctgatc
     4081 gacgacgccg ccttcaccga cctgggcgcc tacggcggcg aagcgcacac gcccaacctg
     4141 gacatgctcg ccgcacgcgg cgccatgttc accagctatc acacctcgcc gctgtgctcg
     4201 ccctcgcgcg ccatgctgct caccggcatc gacaaccaca agaccggcgt cgccaccatc
     4261 ccggaaacgc tgccgtccga acaggtgggc aagcatggct acagcatgtt cctggagccg
     4321 ggcgtcgaca ccgtcgcgca gcacctgaag ggcgccggtt accgcaccta catgagcggc
     4381 aaatggcacc tgggccacgg ccccggccag ctgccggatg gccacggctt cgatcactcg
     4441 ctcgcgctcg acgcctccgg cgccgacaac tggcagcaga aaccctacat gccgtattac
     4501 gcgacggccg actggttcga ggacggcaag ccggtgcagc tgccggagaa ctactactcg
     4561 tcggagatga tcgtcgacca catgatcggc tacctgaagc gcgaccaggc caatgccgcg
     4621 ccgttccttg cctacgtgcc gttccaggca gtgcacatcc cggtgcaggc gccgcgcgaa
     4681 ttcactgcgc actacgccgg cgtgtacgac ggcggctggg acgcgacgcg cgaacgccgc
     4741 tggcagcgcg ccaaggaact gcacctgatc ccggccgatg ccgagctgcc gccggcgcat
     4801 gacaagctgc gcaggtggga cgcgcagagc gccgaggaca aggcgctgtt cgccaagagc
     4861 atggcggtgt acgccggcat gatcgaggcg atggatgcca atgtcggcaa gctgatcgcg
     4921 tacctgaagg aaaccggcca gtacgaccac accatctttt tcgtcacctc cgacaacgga
     4981 cccgagccca gcgatccgct ggcgcaggtc ggcttcgcca cctggatgtc gcagaacggc
     5041 tacgagcgca agatcgacac gctgggcgag aaaggcagca tgaacttcat cggccccgag
     5101 tgggcgaatg cagcggcctc gccggatcgc ctgttcaagt tccactcggc cgaaggcggc
     5161 ctgcgcgtgc cgctgatcgt gtcggggccc ggcgtgtcgc agggcatggt caacagcctg
     5221 agtttcgtca ccgacctcac gccgaccatc tacgactacg cgggcatcga tccggccacg
     5281 tggaacggca aggtgccgct caccggcaag agcctggcgc cggtgctgcg cggccaggcc
     5341 gagcgcacct atgcgcccga cgtgccggtg ggcatcgagg tcggcggtaa cgcggccttg
     5401 ttcaagggcg actacaagct cacgcacatc tcgctgccct ggggcgacgc caagtggcac
     5461 ctgtatcacc tgaccgaaga tcccggcgag gtgcatgacc tcagcacgct ggacgccgag
     5521 cgctacaaaa gcatgctggc cgactacgac aactacgcca agcagatggg cgtgctcaag
     5581 ctgcccgacg acttcaatcc gcacacgcag gtggcgatca acgctctgga gaagcaggtg
     5641 gagtacctgc gggtgaaaat cgcagctgcc gcggtgttca tcatcgccat cgtgatctgg
     5701 ctggtgcggc gccggcgcac tgcggcggat tcctgatttc gccgattacc atgccacgca
     5761 tcaacgtcac cgatgacctg ggcagctgga tggcgctgcg tcccgagatg gcgcctgggc
     5821 tggtggcact gagcaacgcg gtctacggcg cgtcgaagct gccgttgcgc gtgcaggaga
     5881 tcgcgcgcat gcgcatcgcg ctggccgacg gttgcgaggt ctgccgcaac gcgcgctacg
     5941 cctggccgcg gaatacgccg agcgctacgc gctggaccac acgggcctgc gcgaggacga
     6001 cgctttctgg cagcgcctgc gcgcgcagtt ccgcgacgac gagatcgtcg acctggcgct
     6061 gtgctgcacg ctgtggctcg gcgccggccg caccatgcgc gtgctcgatg tcggccagac
     6121 ctgcgcgctg acgctgacgc gcgccgcccg ctgatccacg cgcgccaccc ggcgccgctt
     6181 agtcgccatc ggcacgcgtc caggtgctgg actcgccgag catgtgcaac cccttgtagc
     6241 cgtacaccac cgcacgcccg tggtcgaggc tgatctccac ggtgccggtg acgccctgcg
     6301 tcatgccggg gcgaaggtcg atgacgcgcc cgccgatcca gcgttcgcca tcgcggtgca
     6361 ggttctcgat gaacaccatg ccggccagcg gctggccgcg ccgcgcgccg tcgcagcgct
     6421 cgcagcggcg cagcacctcg gcgccgttgc ggtcacgcac ctgcagcacg cgtccgtaga
     6481 gatggtcgtc ctgcaggtac aactcgacgg tcgagtcaac gccatcgggt tcgggtggtg
     6541 tacgccagcg gccggtgtag tcgccgtcgg ctgcgtgcgc cacgcccggc agcagcaacg
     6601 ctgcgacggc cagcgcacgg cggagtcgcg ctgcggatgc gggctgcatg tcagaaatac
     6661 cggtacacca ggtccagccc cacggtgagc ggctcgcccc acagcacggc acggtcggcc
     6721 tgcggcaggt tgtcgacggc gaacaccacg tagtcgcgat tgagcaggtt gcggccccac
     6781 agcgccactt ccagcgaacc ggtgtcgccc acgtgcaggc cgctggcggc gacgcgcccg
     6841 ctgagcacgc cgtagcccgg caggctggtc aggccgcggc gctgttcggt gatgaccgtg
     6901 ccgatgcgat aacccacgta gttgtagctc aggtacgcac gcaggctgcc
//
LOCUS       DASZZC010000415         8418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_229007,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000415 DASZZC010000000
VERSION     DASZZC010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229007"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VHE37_08825"
     CDS             complement(<1..1256)
                     /locus_tag="VHE37_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35675.1"
                     /translation="MTLFNRFFSRRNALKAGAPVLLGLAGAASADEAAAVPAPFPARI
                     GEQGVLNADVAYGNFLRWVPKEHWPGIASGETDYYADDALRDMTLSCAATGDEALLPR
                     GRIRLRNTWHVWSGSHEVIAVKIRGYGGRTGTGIVSVLRNTWIDGSELRDRPIINIEM
                     ARGVELRGLAITGPNARPHERAAAATRWEPDRARWIGEGCSNGRHNPQCGIAIDAYGG
                     AADATKDGYPEPERYGQHTGNGSSGIRLADISISDCVVGLVDSPSWTNALGDLVDCAG
                     IDLRNNAVCFACGNSNIFNFHFSAANNAATAHTFFDSESYGLGHGCIPHFDRFNIGNM
                     YQLFQCGGVAPSPALIQHCYAEGICRIGSFGIFNQGSAALEQRCSVRFQACHFLFYGK
                     EFDGRHAAMLCESLGNIEFTECKLGLA"
     gene            complement(1290..3914)
                     /gene="alaS"
                     /locus_tag="VHE37_08830"
     CDS             complement(1290..3914)
                     /gene="alaS"
                     /locus_tag="VHE37_08830"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HVT35676.1"
                     /translation="MSKPTTTNQVRAAFLDFFKGKGHAVVPSSPLVPGNDPTLLFTNA
                     GMVQFKDVFTGQDKRPYTRATSSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFG
                     DYFKQDAIAYAWEFITSKEWLGIDPSRLLVTVYETDDEAFDIWHKSVGLPKDRIVRIG
                     DNKGAKYASDNFWAMGDTGPCGPCTEIFYDHGAHIPGGPPGSKDEDGDRWIEIWNVVF
                     MQFERHKDGTLTQLPKPSVDTGMGLERISALMQGTNDNYQIDTFRDLVGAVAALAKQK
                     PYESASQKVVADHIRATSFLIADGVMPSNEGRGYVLRRIMRRAIRHGYKLGLTDAFFY
                     KLVAPLAKEMGDAYPQLREQQQRITDAIRAEEDNFAITLDKGMKLLDEAIAKIKDGVI
                     PGGVVFKLYDTYGFPLDLTADIARERNLKLDLDGYEKEMDAQRERARGASKFAAADGV
                     AYAGADTCFIGYDHLTSKDARVLALYRDGKSVGELKAGEQGIVVLDTTPFYAESGGQV
                     GDQGRIGDFEVADTQKIKGSVFGHHGTLKSGALKIGQAVHAEVTQSARRATMRNHSAT
                     HLLHSALREVLGTHVTQKGSLVDAERTRFDFAHNKPVSADEIARIEDIVNRAVLDNVE
                     VSARQMKYDDAIKAGAMALFGEKYGDTVRVLQMGEHSTELCGGTHVSRTGDIGLFKIV
                     MEGGVAAGIRRVEGITGENALKYLRDSEKKIADAAALVRGSRNDLAEKIQQLIERGKQ
                     VEKELAALKGKMASSTGGDLASQAREIKGVKVLAAQVDGVGGNELRTLMDQLKNKLGS
                     GIVLLGSAADGKVSLIAGVTADLTAKVKAGELVNFVAGQVGGKGGGRPDMAQAGGTQP
                     EHLPQAVASVSGWIESKL"
     gene            4003..5349
                     /locus_tag="VHE37_08835"
     CDS             4003..5349
                     /locus_tag="VHE37_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT35677.1"
                     /translation="MATPEPAAARTAGGTPISKLGKYDIVGELGRGSFGVVYKGVDPF
                     VEREVAIKVADVADDRSAKEIQDAFFAEARAGGRLQHPYIVTLFDAGVEGNHTYLVME
                     YIAGETLKRFTLRDSPRLKLETACNLMLKCALALDYIHKQGVLHKDIKPGNIMVTKGG
                     IPKIMDFSISAVTRAGESQDQTLAGSPLYIAPELLHHKPPSAASDLYSLGVVLYLLLT
                     GEPPFTAREATMLFQQIKHLPPIPLKEHRRDIPDPLADIVMRLLDKDPAKRHASGREL
                     AEDLSPYSEQRKEARRKGLNFSHDSLRKLEFFKAFKSRELGELMDASESVTVRAGNNI
                     ITEGDTDNALYILMMGVAEVRKGGHLMAMLEKGDCFGEIGFLYAVKRTATVTAHTDVM
                     VLKVNSELLETMSQECQLRYYKIFCENLILRLTMTTEKASQVAGTSDDSLLDFTIS"
     gene            complement(5611..6078)
                     /locus_tag="VHE37_08840"
     CDS             complement(5611..6078)
                     /locus_tag="VHE37_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HVT35678.1"
                     /translation="MRRSKDLSAAGARNAALRTLGRREHSAAELSAKLAWRGYDEETV
                     RDTVSSLAGKGWQSDERYAEQLVRSRIAQGYGPLRIEAELAAAGVDKKQVAAAMDSAD
                     CDWRASVRALHQRRFKSAPQGAAEWQKQYRFLAGRGYTSSQIHAVLREAPESD"
     gene            complement(6065..7111)
                     /gene="recA"
                     /locus_tag="VHE37_08845"
     CDS             complement(6065..7111)
                     /gene="recA"
                     /locus_tag="VHE37_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HVT35679.1"
                     /translation="MDDNRKKALEAALQQIERQFGKGAVMRMSTDERPEVEVISTGSL
                     MLDAALGVGGIPRGRVVEIYGPESSGKTTLTLHMVAQAQKAGGVAAFIDAEHALDVTY
                     AEKLGVNVGDLLISQPDTGEQALEIVDMLVRSNSIDVIVVDSVAALVPKSEIEGEMGD
                     ASVGVQARLMSQALRKLTGSINRSKTTVIFINQLRMKIGVLMPGQSPETTTGGNALKF
                     YASMRLDIRRIGSVKKGEEVVGNETRVKVVKNKLAPPFKQVIFEIMYGEGISHEGELI
                     DLGIEHGLIDKSGAWYAYQGEKIGQGKDNSKSYLREHPEIAAEIETALREKLLPGRSA
                     KVVELPTPAARAAK"
     gene            complement(7246..7830)
                     /gene="thpR"
                     /locus_tag="VHE37_08850"
     CDS             complement(7246..7830)
                     /gene="thpR"
                     /locus_tag="VHE37_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HVT35680.1"
                     /translation="MSAQPNRLFFALVPDPEVRAACAAAAREIKLKAQPSGALVPPER
                     YHLTLLFLGSFVTPLQEQALSEAAAALRQAPFSLLLDHASSFARERQVPWWLGPREAS
                     PAMLKLYGALRDAAHRAGITPERSRFAPHLTIVRDARHALAQTAIKPVEWRVREFVLI
                     RSVLDSTPPEYRVIGRWPLVEAAPAEQPAQMKLF"
     gene            complement(7827..8300)
                     /locus_tag="VHE37_08855"
     CDS             complement(7827..8300)
                     /locus_tag="VHE37_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HVT35681.1"
                     /translation="MESLAAEVGNQLLARRQLLALAESCTGGLIAKLVTDVAGSSAWF
                     ERGLVTYSNQSKQDLLGVPAEILRAQGAVSEPCARAMADGLLRGSPAHWGIAVTGIAG
                     PAGGTPEKPVGLVWLALARRGTDTRTRELRLSGDREQIRAHTARTALQWLRDEMA"
BASE COUNT         1330 a         2846 c         2735 g         1507 t
ORIGIN      
        1 gccagtccga gcttgcattc ggtgaattcg atattgccca gcgactcgca cagcatcgcg
       61 gcgtgacgcc cgtcgaattc cttgccgtag aacaggaaat ggcaggcctg gaaacgcacc
      121 gagcagcgct gctcgagggc ggccgagccc tggttgaaga tgccgaacga accgatgcgg
      181 cagatgccct cggcgtagca gtgctggatc aacgccgggc tgggcgcaac gccgccgcac
      241 tggaacaact ggtacatatt gccaatattg aagcggtcga agtgcggaat gcagccatgc
      301 cccagcccgt aagactccga gtcgaaaaac gtgtgcgcgg tggcggcgtt gttggctgcg
      361 ctgaaatgga aattgaagat gtttgaattg ccgcaggcga agcacaccgc gttgttgcgc
      421 agatcgatgc cagcgcaatc gaccaggtcg cccagcgcgt tggtccagga cggcgaatcc
      481 accaggccca ccacgcaatc gctgatcgag atatcggcga gccggatgcc ggaactgccg
      541 ttgccggtgt gctggccgta gcgctcgggc tccgggtagc cgtccttggt cgcatctgct
      601 gcgccgccat aggcatcgat ggcgatgccg cactgcggat tgtggcggcc attgctgcag
      661 ccttcaccga tccatcgcgc gcggtccggc tcccagcgcg tggcggccgc tgcgcgctcg
      721 tgcggacgtg cattcgggcc ggtgatagcg agcccgcgca gctcgacccc gcgcgccatc
      781 tcgatgttga tgatcggccg gtcgcgcagt tcgctgccgt cgatccaggt attgcgcagc
      841 acgctgacga tgcccgtccc ggtgcggcca ccgtagccgc ggatcttcac cgcgatcact
      901 tcgtggctgc cactccacac atgccaggtg ttgcgcaggc ggatgcggcc gcgcggcagc
      961 agcgcctcgt caccggtggc ggcgcacgac agcgtcatgt cgcgcagcgc gtcatcggcg
     1021 tagtaatcgg tctcgccgga ggcgatgccg ggccagtgct ctttcggaac ccagcgcagg
     1081 aaattgccgt aggccacgtc ggcgttgagc acgccctgct cgccgatcct cgccgggaac
     1141 ggcgccggaa cggcggcggc ctcatccgcg ctggccgcgc cggccaggcc cagcaacacg
     1201 ggcgcgccgg ccttgagcgc attgcgccgc gaaaagaaac ggttgaacaa agtcatgggt
     1261 tcatgcgtgg cggcgcaaac ccgcgcgggc tacagcttgc tttcgatcca gccggacacg
     1321 ctggccacgg cctgcggcag atgttcgggc tgcgtgccgc cagcctgcgc catgtccggc
     1381 cggccaccgc ccttgccacc aacctgtccg gccacgaaat tcaccagctc gccggccttc
     1441 accttcgccg tcaggtcggc ggtgacgccc gcgatcaggc tgaccttgcc gtcggctgcg
     1501 ctgcccagca gcacgatgcc ggagccgagc ttgttcttca gctgatccat caaggtgcgc
     1561 aattcgttgc cacccacgcc gtcgacctgc gcggccagca ccttcacgcc cttgatctcc
     1621 ctggcctgcg atgcaagatc accgccggtg gaggaagcca tcttgccctt gagtgcggcc
     1681 agttcctttt ccacctgctt gccgcgctcg atcagctgct ggattttttc cgcgagatcg
     1741 ttgcgcgagc cgcgcaccag tgcggcggcg tcggcgatct tcttctcgct gtcgcgcagg
     1801 tacttcagcg cgttctcgcc ggtgatgcct tccacgcggc ggatgccggc ggcgacgccg
     1861 ccttccatca cgatcttgaa caggccgatg tcgccggtgc gcgacacatg cgtgccgccg
     1921 cagagctccg tggaatgttc gcccatctgc agcacgcgca cggtgtcgcc gtacttctcg
     1981 ccgaacaggg ccatggcgcc ggccttgatg gcgtcgtcgt atttcatctg gcgcgcggag
     2041 acttcgacgt tgtcgagcac cgcgcggttg acgatgtcct cgatgcgcgc gatctcgtcc
     2101 gcgctcaccg gcttgttgtg cgcgaagtcg aagcgcgtgc gctcggcgtc gaccagcgag
     2161 cccttctgcg tcacgtgcgt acccagcacc tcgcgcaggg cgctgtgcag caggtgcgtg
     2221 gccgagtggt tgcgcatggt ggcgcggcgg gcggactggg tgacctcggc gtgcaccgcc
     2281 tggccgattt tgagcgcgcc ggatttcagc gtgccgtgat ggccgaagac gctgcccttg
     2341 atcttctgcg tatcggccac ttcaaaatca ccgatgcggc cctggtcgcc gacctggccg
     2401 ccggactcgg cgtagaacgg cgtggtgtcc agcaccacga tgccctgctc gccggccttc
     2461 agctcgccta cgctcttgcc gtcgcggtac agcgccagca ccctggcgtc ctttgacgtg
     2521 agatggtcgt agccgatgaa gcaggtgtcc gcgcctgcgt aggccacacc atcggccgca
     2581 gcgaacttgc tggcgccgcg cgcgcgttcg cgctgcgcgt ccatttcctt ctcgtatcca
     2641 tccagatcga gcttgagatt gcgctcgcgg gcgatgtcgg cggtgaggtc gagcgggaat
     2701 ccgtaggtgt cgtagagctt gaacacgacg ccgccgggaa tcacgccgtc cttgatcttc
     2761 gcgatcgctt cgtcaagcag cttcatgccc ttgtcgagcg tgatggcgaa gttgtcttcc
     2821 tccgcgcgga tggcgtcggt gatgcgctgc tgctgctcgc gcagttgcgg ataggcgtcg
     2881 cccatttctt tcgccagcgg cgcgaccagt ttgtagaaga acgcgtcggt caggccaagc
     2941 ttgtaaccgt ggcggatggc gcggcgcatg atgcggcgca ggacgtagcc gcggccctcg
     3001 ttgctcggca tcacgccgtc ggcgatcagg aacgaggtgg cgcggatgtg gtctgccacc
     3061 accttctgcg aggcgctttc gtagggcttc tgcttcgcca gcgcagccac ggcgccgacc
     3121 aggtcgcgga aggtatcgat ctggtagttg tcgttggtgc cctgcatcaa cgcgctgatg
     3181 cgctccaggc ccatgccggt gtcgaccgaa ggtttcggca gctgggtcag cgtgccgtcc
     3241 ttgtgccgct cgaactgcat gaacaccacg ttccagatct cgatccagcg gtcgccgtcc
     3301 tcgtccttgc tgccaggcgg gccgcccggg atatgcgcac cgtggtcgta gaagatttcg
     3361 gtgcaggggc cgcaggggcc ggtgtcgccc atcgcccaga agttgtcgga agcatacttc
     3421 gctcccttgt tgtcgccgat gcgcacgatg cggtctttcg gcaggccgac actcttgtgc
     3481 cagatgtcga aggcctcgtc gtcggtttcg tacaccgtga ccagcaggcg cgacgggtcg
     3541 atgcccagcc actccttcga ggtgatgaat tcccaggcgt aggcgatggc gtcctgcttg
     3601 aagtaatcgc cgaaggagaa attgcccagc atctcgaaga aggtgtggtg ccgcgcggtg
     3661 tagcccacgt tctccaggtc attgtgcttg ccgccggcac gcacgcattt ctggctggag
     3721 gtagcgcgcg tgtaggggcg cttgtcctgg ccggtgaaca cgtccttgaa ctgcaccatg
     3781 ccggcattgg tgaacagcag cgtcgggtcg ttgccgggca ccagcgggga cgaaggcacg
     3841 accgcgtgcc ccttgccttt gaagaaatcg aggaaggccg cgcggacctg gttggtggtg
     3901 gtgggcttgg acacggaggg catggcgagt aaagcagggc cgccatggta gccaaacggg
     3961 gcgctgcgcc gccacaatag tccccagggg agatacgcag tcatggccac gcctgaaccc
     4021 gccgcggcgc gcaccgccgg cggcacaccg atcagcaagc tgggcaagta cgacatcgtc
     4081 ggcgagctcg gccgcggcag ctttggcgtg gtgtacaagg gcgtcgaccc cttcgtcgag
     4141 cgcgaggtgg cgatcaaggt agccgacgtc gccgacgacc gcagcgccaa ggaaatccag
     4201 gacgcgttct tcgccgaggc ccgcgccggc gggcggttgc agcatcctta catcgtcacc
     4261 ctgttcgacg ccggcgtgga gggcaaccac acctacctgg tgatggaata catcgccggt
     4321 gaaacgctca agcgcttcac cctgcgcgac tcgccgcggc tcaagctgga gaccgcgtgc
     4381 aacctgatgc tcaagtgcgc gctggcgctg gattacatcc acaagcaggg cgtgctgcac
     4441 aaggacatca agcccggcaa catcatggtg accaagggcg gcattcccaa gatcatggat
     4501 ttctcgatct cggcggtgac gcgcgccggt gaatcgcagg accagacgct cgcggggtcg
     4561 ccgctgtaca tcgcgccgga actgctgcac cacaagccgc ccagcgccgc gtccgacctg
     4621 tattcgctgg gcgtggtgct gtacctgctg ctcaccggcg agccgccgtt caccgcgcgc
     4681 gaagccacca tgctgttcca gcagatcaag cacctgccgc cgatcccgct gaaggaacac
     4741 cgccgcgaca ttcccgatcc gctggccgac atcgtcatgc gcctgctgga caaggacccg
     4801 gccaagcggc acgcctcggg ccgcgagctg gcggaagacc tctcgcccta ctccgagcag
     4861 cgcaaggagg cgcggcgcaa gggcctcaac ttttcccacg actcgctgcg caagctggaa
     4921 ttcttcaagg cgttcaagtc gcgcgaactc ggcgaactga tggacgccag cgaatcggtc
     4981 acggtgcgcg ccggcaacaa catcatcacc gagggcgaca ccgacaacgc gctgtacatc
     5041 ctgatgatgg gcgtggccga ggtgcgcaag ggcggccacc tgatggccat gctggaaaag
     5101 ggcgactgct tcggcgagat cggctttctc tacgcggtga aacgcacggc gacggtcacc
     5161 gcgcacaccg acgtcatggt gctcaaggtc aattccgagc tgctggagac catgtcgcag
     5221 gaatgccagc tgcgctacta taagatcttc tgcgagaacc tgatcctgcg cctgaccatg
     5281 accaccgaga aggcgagcca ggtggccggc acctcggacg acagcctgct ggacttcacc
     5341 atctcgtagc gccgcatggg cgcgcatgcg cgcgccattt cgcgtgccgc ttcgccgcgc
     5401 tttgcgcgcg ggcggcgcac agcgaaaacc tcgcgacccc tgttgcagcg ggtcttcaac
     5461 atccatccgc aggctggaat cacggcctgg aagtactgac cgcaccgccc tttgagctgt
     5521 tattaattat tatctatttt atattttgta ttatttttaa taacaacaga acgggcttgc
     5581 ggttgaccac ccgcacacgc attccgcgtg tcagtcgctc tccggcgctt cacgcaggac
     5641 ggcgtggatc tgtgaggagg tgtaaccgcg cccggcgagg aagcgatatt gcttctgcca
     5701 ttccgctgcg ccctgcggcg ctgatttgaa gcgacgctgg tgcagcgcac gcacgctggc
     5761 gcgccagtcg cagtccgcac tgtccattgc cgcggcgacc tgctttttgt cgacgcccgc
     5821 ggccgccagc tccgcctcga tgcgcagcgg cccgtagccc tgcgcgatgc gcgagcgcac
     5881 cagctgctcg gcgtagcgct cgtcggattg ccagcccttg ccggcgagcg aggagacggt
     5941 gtcgcgcacc gtctcctcgt cgtagccacg ccaggccagc ttggccgata gctcggcagc
     6001 cgaatgctca cggcggccga gcgtgcgcaa cgccgcgttg cgcgcgccag ccgccgacag
     6061 gtccttactt cgccgcacgc gccgccggcg tcggcagctc gaccaccttg gccgacctgc
     6121 ccggcaggag tttctcgcgc agcgcggtct cgatctccgc ggcgatttcc gggtgctcgc
     6181 gcaggtagga cttggagttg tccttgccct gcccgatctt ctcgccctgg tacgcatacc
     6241 aggcgccgga cttgtcgatc agcccgtgct cgatgcccag gtcgatcagc tcgccctcgt
     6301 gcgagatgcc ctcgccgtac atgatctcga agatcacctg cttgaacggc ggcgccagct
     6361 tgttcttcac cactttcacg cgggtttcgt tgcccaccac ttcctcgccc ttcttcacgc
     6421 tgccgatgcg gcggatgtcc aggcgcatgg aggcgtagaa cttgagcgcg ttgccgccgg
     6481 tggtggtttc cgggctctgg cccggcatca gcacgccgat cttcatgcgc agctggttga
     6541 tgaagatgac cgtggtcttg gagcggttga tgctgccggt gagcttgcgc agcgcctggc
     6601 tcatcaggcg tgcctgcacg ccgaccgagg cgtcgcccat ctcgccctcg atttccgact
     6661 tgggcaccag cgcggcgacc gagtcgacca cgatgacgtc gatgctgttg gagcgcacca
     6721 gcatgtcgac gatttccagc gcctgctcgc cggtgtccgg ctggctgatc agcaggtcgc
     6781 cgacattgac gccgagtttt tcggcatagg tcacgtcgag cgcgtgctcg gcgtcgatga
     6841 acgccgccac gccgccggcc ttctgcgcct gcgccaccat gtgcagcgtc agcgtggtct
     6901 tgccggacga ttccggcccg tagatttcca ccacgcggcc gcgcggaatg ccgcccacgc
     6961 ccagcgccgc gtccagcatc agcgaaccgg ttgagatcac ttccacctcc gggcgttcgt
     7021 cggtgctcat gcgcatcacg gcgcccttgc cgaactgcct ttcgatctgt tgcagcgccg
     7081 cttccagcgc cttcttgcgg ttgtcgtcca tcgtcgtacc ctcggaaagt tgttatagga
     7141 gctacggtgc ggttggagac ggcaccggcg ggacctagtg tatggatata caccatcaag
     7201 tcaagcgcgg attcctgcac cgcgcgttgc cggcgcaggg cgctgtcaga acagcttcat
     7261 ctgcgccggc tgctccgccg gcgcggcctc gaccagcggc cagcggccga tcacgcggta
     7321 ttccggcggc gtggaatcga gcacgctgcg gatcagcacg aactcgcgca cgcgccactc
     7381 gaccggcttg atcgcggtct gcgccagcgc atggcgcgcg tcgcgcacga tggtcaggtg
     7441 cggcgcaaag cggctgcgtt ccggcgtgat gccggcgcgg tgcgcggcgt cgcgcagcgc
     7501 gccgtacagc ttgagcatgg ccggcgatgc ctcgcgcggg cccagccacc acggcacctg
     7561 cctctcgcgc gcaaagctcg aagcatgatc cagcagcagg ctgaacggcg cctgccgcag
     7621 cgcggccgcc gcttcgctca gcgcctgttc ctgcagcggc gtaacgaagc tgcccaggaa
     7681 cagcagcgtg aggtgatagc gctccggcgg caccagcgcg ccggaaggct gcgccttgag
     7741 cttgatctcg cgcgccgccg ccgcgcaggc cgcgcgcacc tcggggtcgg gcaccagcgc
     7801 gaagaacagg cgattgggtt gcgcactcac gccatttcat cccgcagcca ttgcagcgcg
     7861 gtgcgcgcgg tgtgcgcacg gatctgttca cggtcgccac tcagccgcag ctcgcgcgtg
     7921 cgcgtgtccg taccgcgacg ggccagcgcc agccacacca gtcccaccgg cttttccgga
     7981 gtgccgccgg ccggcccggc gatgccggtg acggcgatgc cccagtgcgc ggggctcccg
     8041 cgcagcaggc cgtcggccat ggcgcgtgcg cagggctcgc tcaccgcacc ttgcgcccgc
     8101 aggatttccg caggcacgcc gagcaagtcc tgcttcgatt gattggagta cgtgaccagc
     8161 ccgcgctcga accaggccga cgaacccgcc acgtcggtca ccagcttggc gatcagcccg
     8221 ccggtgcagg actcggccag cgccagcagc tgccggcgcg ccagcagctg gttcccgact
     8281 tcggcggcga gcgattccat gccggcgcct acttcctgaa gatttcctcg gccatgcggt
     8341 cggcgatctc gccggtcggc cggccgccgg cgcgcgaacg ctggtagatg gtggcgagcg
     8401 tgccgccgat gcgctgca
//
LOCUS       DASZZC010000416         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1253229,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000416 DASZZC010000000
VERSION     DASZZC010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253229"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VHE37_08860"
     CDS             complement(<1..375)
                     /locus_tag="VHE37_08860"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35682.1"
                     /translation="MKRVWHRRLRRKAINGVIRSYARLGEYVAEMRAARKKVEVLRDV
                     TYGPNPYNKLDIYRPHFSPRPLKVLLYIHGGGFMLCSKETHRGIALTSAARSNYLVFN
                     IDYRLAPHFQFPGAHEDACRAYA"
     gene            complement(652..1218)
                     /gene="dcd"
                     /locus_tag="VHE37_08865"
     CDS             complement(652..1218)
                     /gene="dcd"
                     /locus_tag="VHE37_08865"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HVT35683.1"
                     /translation="MSIKSDRWIRRMAEQHKMIEPYEPGQVRASGNGRIVSFGTSSYG
                     YDVRCADEFKIFTNINSTIVDPKNFDARSFVDFKGECCVIPPNSFALARTVEYFRIPR
                     SVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLFL
                     ESDEICETSYRDRGGKYMGQKGVTLPKT"
     gene            complement(1218..>1549)
                     /locus_tag="VHE37_08870"
     CDS             complement(1218..>1549)
                     /locus_tag="VHE37_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35684.1"
                     /translation="PMSDSAILRQIQLSQGGRFYHWETPGMPPIPPLVINSHAANTHL
                     IPASHYVLDQLHTMRVGQVIELGGYLVDVTRADGWHWNSSLVRTDSGAGACELMYVES
                     ATIVRGE"
BASE COUNT          252 a          486 c          506 g          305 t
ORIGIN      
        1 ggcgtaggcg cggcaggcgt cctcatgcgc gccgggaaac tggaagtgcg gcgccagccg
       61 gtagtcgatg ttgaacacca ggtagttcga gcgcgccgcg ctggtcaggg cgataccccg
      121 gtgcgtctcc ttcgagcaca acatgaaacc accgccgtgg atgtacagca gcaccttgag
      181 cgggcgcggc gagaaatgcg ggcggtagat gtcgagcttg ttgtagggat tggggccgta
      241 ggtgacatcg cggagcacct ccaccttctt gcgcgcggcg cgcatctcgg ccacgtattc
      301 acccaggcgc gcgtagctcc tgatcacgcc gttgatcgcc ttgcggcgca ggcgccggtg
      361 ccagacgcgt ttcatcggta ccgctgcggc ttgcgccatg tatcacctct cccttgttct
      421 gacacttccc cagtgtgcgg acgccggtcg aacttttcat tggatatttc atcggcgcga
      481 ccgctggtca tcccgcaagt tccgctcatg tcttcggcag cgtgactcct atctggccca
      541 tgcacttgcc tccccgggag ctgtcgcgac acgagctacc cggcgaggga cccgcttgcg
      601 ggatcgccgg cagagcgagt ggcgcagtcg cgaccgcatt cccgctgccc gctacgtctt
      661 cggcaatgta acccccttct gacccatgta tttgccgccg cggtcgcgat aggacgtttc
      721 gcagatctcg tcggattcca ggaacagcat ctgcgccacg ccctcgttgg cgtagatctt
      781 cgccggcagc ggcgtggtgt tggagaactc cagcgtcacg tggccttccc attccggctc
      841 cagcggcgtg acgttgacga tgatgccgca gcgcgcgtag gtgctcttgc ccaggcagat
      901 ggtcagcacc gagcgcggga tgcggaaata ctcgacggtg cgcgccagag caaaggagtt
      961 gggcgggatc acgcagcact cgcccttgaa gtcgacgaag ctgcgtgcgt cgaagttctt
     1021 cggatcgacg atggtggaat tgatgttggt gaagatcttg aattcgtcgg cgcagcgcac
     1081 gtcgtagccg tacgacgacg tgccgaaact gacgatgcgg ccattgccgc tggcgcggac
     1141 ctggcccggc tcgtacggct cgatcatctt gtgctgctcg gccatgcggc ggatccagcg
     1201 gtcggacttg atgctcatca ctcccctcgg actatggttg cgctttccac gtacatcagt
     1261 tcgcaggcgc cggcgccgct gtcggtacgg accagcgaac tgttccagtg ccagccgtcg
     1321 gcgcgggtca catcgaccag gtagccgccc agctcgatca cctggccgac gcgcattgta
     1381 tgcagctggt cgagcacgta gtggctggcg ggaatcaggt gcgtgttggc ggcatggctg
     1441 ttgatcacca gcggcggaat cggcggcatg cccggcgttt cccagtgata gaaacgcccg
     1501 ccctgcgaca gctggatctg ccggaggatg gcgctgtcgg acatcgggc
//
LOCUS       DASZZC010000417         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1129488,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000417 DASZZC010000000
VERSION     DASZZC010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129488"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VHE37_08875"
     CDS             <1..683
                     /locus_tag="VHE37_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVT35685.1"
                     /translation="APPFCSLFSAVGAGNMNQMHIHEISTWTVLFNSNLKTFFSDYAS
                     FNRDVEELERRGREDLLRQGFAAKDIRYRLEIDMRYGNQRVQTAVVSEISRLNSQADV
                     LKLINQFHTSYGQRFGEGSQSPEAGVRINTLRVCSYVEQSTVKFSGLKLNGKSRPPPP
                     PVGQRDCYFVGQAKPLLTTVYDDRALAEGTAIRGPAIVTTRATTYLVEPGWRYQAAAQ
                     NAVWFLRA"
     gene            703..2970
                     /locus_tag="VHE37_08880"
     CDS             703..2970
                     /locus_tag="VHE37_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HVT35686.1"
                     /translation="MHDTISKEEQALLDQFLADNRLFLGPDPELMRNHSIAPRTPQED
                     AILKKGLDPHHVHQIRGLLNAALSEGFQMVNQMGAAPGAKWGDLVTGVYTTSGDLAMI
                     APHGIIAFAACCFYPIKFIIKNWMNDPTVGVKDGDGFIHNDARYGGIHNTDQSMMMPL
                     YYKGELVCWLSSTVHEGENGACEPGGMPAAAESKYDEGIKMSPFKVVENFQLKRDLVT
                     FLQNSVRDPKLQLEDMKVKLHAVMRLRERVIAILDQYGKEALLGTLRTHLEDVETEMR
                     RRIRELPDGTTRVAAFMDSSLRENAMQKIQCAITVKGERMILDFRGTAPQFMNRSVNT
                     NIASFKAALCTGLLQNIWPDLPHTMAVLSPIEIVTDRNSIIDAEGEMPQAMSLMPLFK
                     ACVIWTIPMNKLNYSVKRYSAIIASQYDQAATFIYGGLTQHGDVTGNFCGDINGNGQG
                     ARSDADGEHSVSPVFGFMCDSGEHEINEEDTPIIRLGAQRLAKDRIGFGKYRGGLGYE
                     QIATARGSGMWGFMTGCEGSKFPSAQGLFGGYSCPAYQLMKIKGINVFEVLKEKPDTI
                     KEFDILHLMNDQPIPGGKYSSHDMGMTFELCNEGEIYMICQGTGGGYGDVLERDPALV
                     MKDLAEDLMSHDNARDIYKVVYDEKTLIVDEQATADLRAAYRQERIRRGKPFDQFCKE
                     WVTKEPPAHIPYYGSWDNDKEIYATTMLQRVKMPADQLQGAFMVNPKDLRIGALEAEV
                     EKLKTELADCQRARK"
     gene            3083..>3578
                     /locus_tag="VHE37_08885"
     CDS             3083..>3578
                     /locus_tag="VHE37_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT35687.1"
                     /translation="MTDYGKQFRLDGKAALVTGAARGIGAEVALALAGSGAAVLVSDI
                     ADSAGRETVERIRKAGGRAEFVHHEVTSEPQWEAAIALAVKTYGKLDVLVNNAGILRD
                     ATLWKMSDADFDAVLTTHLRGTFTCTRAAAVHMRGRGGGGRIICVGSPTGQLGNFGQT
                     NYAAA"
BASE COUNT          727 a         1191 c         1137 g          523 t
ORIGIN      
        1 tggcgccgcc gttctgctcg ctgttctcgg cggtgggcgc cggcaacatg aaccagatgc
       61 acatccacga gatcagcacc tggaccgtgc tgttcaactc caatctcaag accttcttca
      121 gcgactacgc cagcttcaac cgcgacgtgg aggaactgga gcggcgcggg cgcgaagacc
      181 tgctgcgcca gggctttgcc gccaaggaca tccgctaccg gctggagatc gacatgcgct
      241 acggcaacca gcgcgtgcag accgcggtgg tgtcggaaat ctcgcggctc aattcgcagg
      301 ccgacgtgct caagctgatc aaccagttcc acaccagcta cggccagcgc ttcggcgaag
      361 gcagccagag ccccgaggcc ggcgtgcgca tcaacacgct gcgcgtgtgc tcgtatgtcg
      421 agcagtccac ggtgaagttc tcgggactca agctcaatgg caagtcgcgc ccgccgccgc
      481 caccggtggg ccagcgcgat tgctacttcg tcggccaggc caagccgctg ctgaccacgg
      541 tgtacgacga ccgcgcgctg gccgaaggca ccgccatccg gggtcccgcc attgtcacca
      601 cgcgcgccac cacctacctg gtcgagccgg gctggcgcta ccaggccgcg gcccagaacg
      661 cggtgtggtt cctgcgcgcc tgaagtccac gaggagaacg atatgcacga caccatcagc
      721 aaggaagagc aggcactgct cgaccagttc ctcgccgaca accgcctgtt cctcggcccc
      781 gacccggagc tgatgcgcaa ccacagcatc gcaccgcgca cgccgcagga agacgccatc
      841 ctgaaaaagg gactcgatcc gcaccacgtg caccagatcc gcgggctgct caacgcagcg
      901 ctgtcggaag gcttccagat ggtcaaccag atgggcgccg cgcccggcgc caagtggggc
      961 gacctggtca ccggcgtgta caccaccagc ggcgacctgg cgatgatcgc gccgcacggc
     1021 atcatcgcgt ttgccgcctg ctgcttctac ccgatcaagt tcatcatcaa gaactggatg
     1081 aacgatccga ccgtgggtgt gaaagacggc gacggtttca tccacaacga cgcgcgctac
     1141 ggcggcatcc acaacaccga ccagtcgatg atgatgccgc tctactacaa gggcgagctg
     1201 gtgtgctggc tgtcgtccac cgtgcacgag ggcgagaacg gcgcctgcga acccggcggc
     1261 atgccggcgg cagcggagag caagtacgac gaaggcatca agatgtcgcc cttcaaggtc
     1321 gtcgagaact tccagctcaa gcgcgacctg gtcaccttcc tgcagaactc ggtgcgcgat
     1381 ccgaaactgc agctggagga catgaaggtc aagctgcacg cggtgatgcg cctgcgcgaa
     1441 cgcgtcatcg ccatcctcga ccagtacggc aaggaagcgc tgctcggcac cctgcgcacg
     1501 catctcgagg acgtggaaac cgagatgcgc cggcgcatcc gggagctgcc cgacggcacc
     1561 acgcgcgtcg ccgccttcat ggattcctcg ctgcgcgaga acgccatgca gaagatccag
     1621 tgcgcgatca cggtcaaggg cgagcgcatg atcctggatt ttcgcggcac cgcgccgcag
     1681 ttcatgaacc gctcggtcaa caccaacatc gcctcgttca aggccgcgct gtgcacgggc
     1741 ctgctgcaga acatctggcc cgacctgccg cacaccatgg cggtgctgtc gccgatcgag
     1801 atcgtcaccg accgcaacag catcatcgat gccgaaggcg aaatgccgca ggccatgagc
     1861 ctgatgccgc tgttcaaggc ctgcgtcatc tggaccatcc cgatgaacaa gctcaactac
     1921 agcgtgaagc gctacagcgc gatcatcgcc tcgcagtacg accaggccgc caccttcatc
     1981 tacggcggcc tgacccagca cggcgacgtc accggcaact tctgcggcga catcaacggc
     2041 aacgggcagg gcgcgcgcag cgacgccgac ggcgagcatt cagtctcgcc cgtgttcggc
     2101 ttcatgtgtg actcgggcga acacgagatc aacgaggaag atacgccgat catccggttg
     2161 ggcgcccagc ggctggcgaa agaccgcatc ggcttcggca agtatcgcgg cggcctgggc
     2221 tacgagcaga tcgccaccgc gcgcggctcc ggcatgtggg gtttcatgac cggttgcgag
     2281 ggttccaagt ttccctcggc gcagggcctg ttcggcggct actcctgccc ggcctaccag
     2341 ctgatgaaga tcaagggcat caacgtgttc gaagtgctga aggagaagcc ggacacgatc
     2401 aaggaattcg acatcctgca cctgatgaac gaccagccca tccccggcgg caagtacagc
     2461 agccacgaca tgggcatgac cttcgagctg tgcaacgagg gcgagatcta catgatctgc
     2521 cagggcaccg gcggcggcta cggcgacgtg ctggagcgcg atcccgcgct ggtgatgaaa
     2581 gacctggccg aggacctcat gtcgcacgac aacgcgcgcg acatctacaa ggtcgtgtac
     2641 gacgagaaga cgctgatcgt cgacgagcag gccaccgcgg acctgcgcgc cgcctaccgg
     2701 caggaacgca tccggcgcgg caagcccttc gaccagttct gcaaggaatg ggtgaccaag
     2761 gaaccgcccg cgcacatccc gtactacggc agctgggaca acgacaagga aatctatgcg
     2821 accaccatgc tgcagcgggt gaagatgccg gccgaccagc tgcagggcgc gttcatggtc
     2881 aatccgaaag acctgcgcat cggcgcgctg gaggcggaag tggagaagct gaagaccgaa
     2941 ctcgcggatt gccagcgggc gcgtaaatga aacccgtcat tcccgcgaag gcgggaatcc
     3001 agttgatcct tattgctgcc gcaaatcaaa actggatccc cgccttcgcg ggtgatggcg
     3061 gcaaataggt gaggagaaga cgatgaccga ctacggcaag cagttccgtc tcgatggcaa
     3121 ggccgcactg gtcaccggcg cggcgcgcgg catcggcgcg gaagtggcgc tggcgctggc
     3181 gggcagcggc gccgcggtgc tggtcagcga catcgccgac agcgccgggc gcgaaacggt
     3241 ggagcgcatc cgcaaggccg gcggccgcgc cgagttcgtc caccacgaag tgaccagcga
     3301 gccgcagtgg gaagccgcca tcgcgctggc ggtgaagacc tacggcaagc tcgacgtgct
     3361 ggtcaacaat gccggcatcc tgcgcgacgc caccctgtgg aagatgagcg acgccgattt
     3421 cgacgcggtg ctgaccaccc atctgcgcgg cacgttcacc tgtacccggg ccgccgcggt
     3481 gcacatgcgc gggcgcggcg ggggcgggcg gatcatctgc gtcgggtcgc cgaccgggca
     3541 actgggcaac ttcggccaga ccaactacgc cgccgcca
//
LOCUS       DASZZC010000418         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_164201,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000418 DASZZC010000000
VERSION     DASZZC010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164201"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1410
                     /locus_tag="VHE37_08890"
     CDS             133..1410
                     /locus_tag="VHE37_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT35688.1"
                     /translation="MKPIGFAAFLCLLGCTPALALTLDCGRLLDVKSGQWREHVSISV
                     IGARIISVEAQSGKAADHVDLSHYACLPGLIDMHVHLTDQISSQAENFRNQLTLEQTD
                     YAYRSVGYAEKTLLAGFTTVRDLGAERNLNISLKRAIDRGWVRGPRMFTSGTPLGSTG
                     GHADPTDGYKREYAPEAGPRDGIVDSVDDARKAVRQHYKDGADLIKVMVTGGVLDLSA
                     SAQNPQLTDAEMQAIVSTAKDYGFRVAVHAHGAEGIKRALRAGADSIEHGTFMDDEAI
                     ALFKQHNAWYVPTIIAGKTVAAKAAIDGFFPPSVQPKALAVGPRIQETFGRAYKAGVK
                     IAFGTDAGVYEHGNNAAEFGYMVDAGMPPLDAIRAATLSAAQLLDKSDALGQVETDFL
                     ADIIAVEGDPLQDVSTLKSVKFVMKDGVIYKTP"
     gene            complement(1414..2706)
                     /locus_tag="VHE37_08895"
     CDS             complement(1414..2706)
                     /locus_tag="VHE37_08895"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT35689.1"
                     /translation="MNDLVPHALVSLVALGMGLAFLSADRESRTSRALAAGLAFIGLS
                     IYLNVAFIRDLSHPPALSRWLALPEVFSMILILEWLLRVRQTLPAGDYNTRFGDRVLR
                     VGQGAGLLYMVFALVWPEIRTGQFLRAGEDPHFYTRPGFWMFMGPVLVATLAGLVGIL
                     LLLNRKPDKAERIRVLAMAAAVPFFTASFVLPLSESAIVVVIGEIIFFVGAVHYHVLQ
                     GQRGQFMSRFLSPQVAQLVNERGLKTAMQENFLEITVVCCDLRGFTSYAQAHPSSRVL
                     AVLREYYDGVGQIVADFGGTIKDFAGDGILILVGAPLPVEHHARCGIEMARRIRAFCI
                     EMTRRWSSEGNTLGIGIGVASGKVTVGVIGSASRLEYTAVGSAVNLASRLCEQAQHCE
                     ILIDRRTVELAGEDGLESRATLAVKGFAEPVPSYALPG"
     gene            complement(2743..>3605)
                     /locus_tag="VHE37_08900"
     CDS             complement(2743..>3605)
                     /locus_tag="VHE37_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HVT35690.1"
                     /translation="VLYALARLLQLPRPSAQSLAFALPQGGEFAFVLYAAAVEAGVMS
                     PQQQSLLVVVVTVSMAITPVLYALHVRWRREEPVRPYDPIDAPENPVIIAGFGPFGQI
                     VGRVLRLKKIGFTVLEKDSEQVDFMRGFGTRVYYGDASRLELLRAAHAHKARWLVLAV
                     AQQDTSLAIAETVRRNFPQLRIYAVARTRQHALRLLDLGVEQVIRRGYFSSLEMVRGL
                     LLALGDSPSAAARAIDMFREADQRTLERQRAVAHDEQAVIQTGRESARELEQLFEEDA
                     AASAEQAPGA"
BASE COUNT          657 a         1252 c         1177 g          519 t
ORIGIN      
        1 cgcgcggatg atacccggct tccctgccgg gggatatcga gggttttacc cagcgacgcc
       61 ggagattttt tcctacactg aggccagcgg tgggaatctc ctgacgagcg gttcagctga
      121 ccggatacgc gcatgaaacc aatcggcttt gcggcatttt tgtgtctgct gggttgcact
      181 ccagcgctgg cgctgaccct ggactgcggg cgcctgctcg acgtgaagtc cggccagtgg
      241 cgcgaacacg tgtcgatcag cgtgatcggc gcacgcatca tttcggtgga agcgcagtcg
      301 ggcaaggctg ccgatcacgt ggacctgagt cactacgcct gcctgcccgg cctgatcgac
      361 atgcacgtgc acctgaccga ccagatcagc agccaggccg aaaatttccg taaccagttg
      421 acgctcgaac agaccgacta cgcctatcgc agcgtgggct atgccgagaa gaccctgctg
      481 gccggcttca ccaccgtgcg cgacctgggc gccgagcgca acctcaacat ctcgctcaag
      541 cgcgccatcg accgcggctg ggtgcgcggc ccgcgcatgt tcacgtccgg cacgccgctg
      601 ggctccaccg gcggccatgc cgatcccacc gacggctaca agcgcgagta cgcgcccgag
      661 gccggcccgc gcgatggcat cgtcgacagc gtggacgatg cgcgcaaggc ggtgcgccag
      721 cactacaagg acggcgccga cctgatcaag gtgatggtca ccggcggcgt gctcgacctt
      781 tctgccagcg cgcagaaccc gcaactgacc gacgccgaga tgcaggccat cgtcagcacg
      841 gcgaaggact acggcttccg cgtggccgtg cacgcgcacg gcgccgaggg catcaagcgc
      901 gcgctgcgcg cgggcgccga ttccatcgag cacggcactt tcatggacga cgaggcgatc
      961 gcgctgttca agcagcacaa cgcctggtac gtgccgacca tcatcgccgg caagacggtg
     1021 gcggcgaagg cggccatcga cggcttcttc ccgccatcgg tccagcccaa ggcgctcgcg
     1081 gtcggcccgc gcatccagga gaccttcggc cgtgcctaca aggcgggtgt gaaaatcgcc
     1141 ttcggcaccg atgccggcgt ctacgagcac ggcaacaacg cggccgagtt cggctacatg
     1201 gtcgacgccg gcatgccgcc gctggacgcg atccgcgccg ctacgctgtc ggccgcgcag
     1261 ctgctggaca agagcgatgc gctgggccag gtggaaaccg atttcctcgc ggacatcatc
     1321 gcggtggaag gcgacccgct gcaggatgtg tccacgctca agagcgtgaa gttcgtgatg
     1381 aaggacggcg tgatctacaa gacgccgtaa gtatcacccc ggcagcgcgt aactcggcac
     1441 cggttcggcg aaacccttca ccgccagcgt ggcgcgcgat tccaggccgt cttcgccggc
     1501 cagttccacc gtgcggcggt cgatcaggat ctcgcaatgc tgcgcctgct cgcacagtct
     1561 cgatgcgaga ttcactgcgg agcccaccgc ggtgtactcc agccgcgagg ccgagccgat
     1621 cacgcccacc gtgaccttgc cgctggccac gccgatgccg atgcccagcg tgttgccttc
     1681 gctgctccag cgccgcgtca tctcgatgca gaacgcgcgg atgcgccgcg ccatctcgat
     1741 gccgcaacgc gcgtggtgtt cgaccggcag aggcgcaccg accaggatca ggatgccgtc
     1801 gccggcaaaa tccttgatgg tgccgccgaa gtccgcaacg atctggccga cgccgtcgta
     1861 gtactcgcgc agcaccgcca gcacgcgcga cgacgggtgc gcctgcgcat acgaggtgaa
     1921 gccgcgcagg tcgcagcaca ccacggtgat ctccaggaaa ttctcctgca tcgcggtctt
     1981 cagcccgcgc tcattcacca gctgggccac ctgcggcgac aggaagcgcg acatgaactg
     2041 cccgcgctgg ccctgcagca cgtggtaatg caccgcaccg acgaagaaga tgatctcgcc
     2101 gatgacgacg acgatcgccg attccgacag cggcagcacg aagctggcgg tgaagaacgg
     2161 caccgccgcc gccatggcca gcacgcggat gcgctcagcc ttgtccggct tgcggttgag
     2221 cagcagcagg atgccgacca ggccggccag cgtcgccacc agcaccgggc ccatgaacat
     2281 ccagaatccc gggcgcgtgt agaaatgcgg atcttcaccg gcgcgcagga actgcccggt
     2341 gcggatctcg ggccagacca gcgcaaacac catatatagg agtccggcgc cctggcccac
     2401 gcgcagcacg cggtcgccga agcgcgtgtt gtagtcgccc gcgggcaatg tctgccgcac
     2461 gcgcagcagc cactcgagga tcaggatcat cgagaacact tcgggcagcg ccagccagcg
     2521 cgacaaagcc ggcggatgcg agagatcgcg gatgaaagca acgttgagat agatcgacag
     2581 gccgatgaac gcgagccccg ccgccagcgc gcgcgaggtg cgtgattcgc ggtcggctga
     2641 gaggaacgcc agccccatgc cgagcgcgac cagcgacacc agcgcatgcg ggacgagatc
     2701 gttcacggcg gcgagcatac gccgcacaac agcggcctgg tgttacgcac cgggcgcctg
     2761 ctccgcgctg gcggcggcat cttcctcgaa caactgctcc agttcgcgcg ccgattcgcg
     2821 gccggtctgg atcaccgcct gctcgtcgtg cgccaccgcg cgctggcgct ccagcgtgcg
     2881 ctgatcggcc tcgcggaaca tgtcgatggc gcgggcggca gccgacgggc tgtcgcccag
     2941 ggccagcagc agtccgcgca ccatttccag gctggagaaa tagccgcggc ggatgacctg
     3001 ctccacgccc aggtcgagca ggcgcagtgc gtgctggcgc gtgcgcgcca ccgcgtagat
     3061 gcgcagctgc gggaagttgc gccgcacggt ttcggcgatg gccaggctgg tgtcctgctg
     3121 cgccaccgcc agcaccagcc agcgcgcctt gtgcgcgtgc gcggcgcgca gcagttccag
     3181 ccgcgacgca tcgccgtaat agacccgcgt gccgaagccg cgcatgaagt cgacctgctc
     3241 cgaatccttc tccagcacgg tgaagccgat cttcttcagg cgcagcacgc ggccgacgat
     3301 ctgcccgaaa ggaccgaagc cggcaatgat caccgggttc tccggcgcgt cgatcggatc
     3361 gtacggccgc accggttcct cgcggcgcca gcgcacatgc agcgcgtaga gcaccggcgt
     3421 tatcgccatc gataccgtga ccaccaccac cagcagcgac tgctgctgcg gcgacatcac
     3481 gcccgcttca acggcagcgg catagagcac gaaggcgaat tcgccaccct gcggcagcgc
     3541 aaaggcgagc gactgtgccg acgggcgcgg cagctgcagc aggcgcgcga gcgcgtagag
     3601 cacgg
//
LOCUS       DASZZC010000419         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_550469,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000419 DASZZC010000000
VERSION     DASZZC010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550469"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VHE37_08905"
     CDS             complement(<1..435)
                     /locus_tag="VHE37_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT35691.1"
                     /translation="MTLALQFLGATGTVTGSRYRVSASGGSFLVDCGLFQGYKQLRLR
                     NWSRLPLPARELAGVVLTHAHIDHSGYLPLLVKQGFRGPVYCTAATAELCRILLPDAG
                     RLQEEEAENANRHGWSRHKPALPLYSEADAISALEYLEPRAIH"
     gene            complement(440..1039)
                     /locus_tag="VHE37_08910"
     CDS             complement(440..1039)
                     /locus_tag="VHE37_08910"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVT35692.1"
                     /translation="MAAPVVPRHILILQGHPERGAQRLCHALARAYANGAREGGHQTE
                     TIDIADLDFPLLHSKQDWEHGTPPAPIVQVQAAIARADHLVLIYPLWLGCMPALLKGF
                     LEQVLRPGFAISPLHDGMPRKLLQGKSARIVVTMGMPGPVYRWYFGAHSLKSLKRNIL
                     GFCGVSPIRDLVVGMVESASEEQRQQWLRKLHQYGLGAM"
     gene            complement(1106..>2258)
                     /locus_tag="VHE37_08915"
     CDS             complement(1106..>2258)
                     /locus_tag="VHE37_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HVT35693.1"
                     /translation="GVRSFALIALAGTACALLAERASSSALLAAGALVVGAVLLSAAR
                     VNLQNTADAGTTTTMAGLLCYALGAMVWHGEAQLAAALALVATMLLYFRSELHSVTRR
                     LTRQDLLSLLQFSVITLIVLPVLPDHGYGPYAAFNPYQIWLMVVIVVAVSLSGYATLR
                     LLGERKGVAVIGLLGGLVTSTATTLVFSRHVRRGSATLAVAQLVILTANLVVLVRLAM
                     LTLLIAPAVLPRLLPVFLPALLLGALVPLRAWLKLDRREQSPALDVSNPTELPMALGV
                     GAVFALVLLATSWLDDRVGSAGVYGMAALMGLTDLDAITLSTLRLVNLGQLQPAQAVT
                     AIVIAFCSNLGFKLAAVLALAGRALWRQVASGYAMVAIGLVGAWLLVIR"
BASE COUNT          403 a          781 c          771 g          303 t
ORIGIN      
        1 atggatggcg cgcggctcca ggtattcgag cgcgctgatg gcgtcggcct cggagtacag
       61 cggcagtgcc ggcttgtgcc gcgaccagcc gtggcggttg gcgttttccg cttcctcttc
      121 ctgcagccgc ccggcatcgg gcagcagaat gcggcacagt tcggcggtag cggccgtgca
      181 gtagaccggc ccgcggaatc cctgcttgac cagcagcggc agataaccgc tgtgatcgat
      241 atgcgcatgg gtcaacacca ccccggccag ttcgcgcgcc ggcagcggca ggcgcgacca
      301 gttgcgcagg cgcaattgct tgtagccctg gaacagcccg caatcgacca ggaaactgcc
      361 gcccgacgcg ctcacccggt aacgcgaacc ggtgacggtg ccggtggcgc cgagaaactg
      421 cagcgcgagc gtcatggcgc tacatcgcgc ccaagccgta ctggtgcagc ttgcgcagcc
      481 attgctggcg ctgctcctcg ctggccgatt cgaccatgcc gaccaccagg tcgcggatgg
      541 gcgacacgcc gcagaatccc aggatgttgc gcttgaggct cttcaggctg tgcgcgccga
      601 aataccagcg gtacaccggg ccgggcatgc ccatggtgac gacgatgcgc gccgacttgc
      661 cctgcagcag cttgcgcggc atgccatcgt gcagcggcga aattgcgaac cccggccgca
      721 atacctgctc taggaatccc ttgagcagcg caggcatgca gcccagccac agcgggtaga
      781 tgagcaccag gtgatcggcg cgcgctatcg ctgcctgtac ctgcacgatg ggtgcgggcg
      841 gcgtgccgtg ctcccagtcc tgcttgctgt gcagcagcgg aaaatcgaga tcggcgatgt
      901 cgatggtttc ggtctggtgg ccgccctcgc gcgccccgtt ggcataggcg cgggccagcg
      961 cgtggcacag gcgctgcgcg ccgcgctcgg ggtgcccctg caggatcagg atgtgcctgg
     1021 gaacaacagg tgcggccatg cgcggaagct ccggaaacga cgcacgcact atgggatgca
     1081 cctgccctgc gggcattgat ctgcgtcagc ggatcaccag cagccacgca cccaccagcc
     1141 cgatggcgac catggcatag cccgaggcaa cctggcgcca cagcgcgcgg ccggccagcg
     1201 ccagcactgc ggccagcttg aagccgaggt tggagcagaa ggcgatgacg atggcggtca
     1261 ccgcctgtgc cggctgcagc tggcccaggt tgaccaggcg caaggtcgac agcgtgatgg
     1321 catcgagatc ggtcaggccc atcagcgccg ccatgccgta gaccccggca ctgcctacac
     1381 ggtcgtcgag ccacgacgtg gcgagcagca ccagtgcgaa caccgcaccg acgcccagcg
     1441 ccatcggcag ttcggtggga ttggagacgt cgagcgccgg gctctgttcg cgccgatcga
     1501 gcttcagcca ggcgcgcagc ggcaccagtg cgcccagcag cagggccggc aggaacaccg
     1561 gcaacagccg cggcagcacc gccggcgcaa tcagcagggt gagcatggcc agccgcacca
     1621 gcacgaccag gttggccgtg aggatcacca gctgcgccac cgccagagtc gccgagccgc
     1681 gccgcacgtg gcgcgaaaac accagcgtgg tcgccgtgct ggtgaccagc ccgccgagca
     1741 ggccgatcac cgccacgccc ttgcgttcgc ccagcaggcg cagcgtggcg tagcccgaca
     1801 ggctcaccgc caccacgatc accaccatca gccagatctg gtacgggttg aatgccgcgt
     1861 aggggccgta gccatggtcc ggcagcaccg gcagcacgat cagcgtgatc accgagaact
     1921 gcagcagcga cagcaggtcc tgccgggtca ggcgccgcgt gacgctgtgc agctcggaac
     1981 ggaaatacag cagcatggtc gccaccagtg ccagcgcggc ggcgagctgc gcttcgccgt
     2041 gccagaccat ggcgccgagc gcgtagcaca gcagcccggc catggtggtg gtggtgccgg
     2101 cgtcagcggt gttctgcaaa ttgacccgcg ccgccgacag cagcaccgcg ccgaccacca
     2161 gcgcgccggc agcgagcaag gcactcgacg atgcgcgctc ggccagcagt gcgcaggcgg
     2221 tgccggccag cgcgatcagc gcaaagctgc gcacgccg
//
LOCUS       DASZZC010000420         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_290774,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000420 DASZZC010000000
VERSION     DASZZC010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290774"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..823
                     /locus_tag="VHE37_08920"
     CDS             194..823
                     /locus_tag="VHE37_08920"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HVT35694.1"
                     /translation="MRTRIKFCGITRAADAQAAAALGVDALGFVLVPASKRWIDPVRA
                     ARIRRALPPLISVVALFKDAAAAQVQEAIDELDPDLLQFHGDEDADYCASFGKPYLKA
                     VSMQQPRALVRAAREHQHAAALLLDSHAPGGMGGSGQIFDWKAVPKVKQPLMLAGGLT
                     PANVGAAVRALRLWAVDVSSGVESKPGIKDTAKMRAFVHAVQRADARSR"
     gene            820..>1958
                     /gene="trpB"
                     /locus_tag="VHE37_08925"
     CDS             820..>1958
                     /gene="trpB"
                     /locus_tag="VHE37_08925"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HVT35695.1"
                     /translation="MNYHYPDSRGHFGPYGGQFVAETLMEPLARLTEAYEKLKNDPAF
                     LAELDDDLRHYVGRPSPLYHAQRLSQKWGGAQVWLKREDLNHTGAHKINNTVGQALLA
                     KHLGKTRIIAETGAGQHGVASATIAARLGLKCVVYMGAEDIQRQSPNVYRMKLLGAEV
                     VPVHSGTRTLKDALNEALRDWVTNVDDTFYVIGTVAGPHPYPMLVRDFQSVIGREVIQ
                     QAHTQIGRLPQALVACVGGGSNAIGLFYPFIEHDEVKMYGVEAGGRGVGTQDHAAPLS
                     AGKPGILHGNRTYVMADANGQIMPTHSISAGLDYPGVGPEHSWLKDLDRVMYTAVNDD
                     DALAAFHELTRVEGIIPALESSHAIAYAKELAAKMPQDQHIVVNLS"
BASE COUNT          369 a          629 c          645 g          315 t
ORIGIN      
        1 cggccccgag tacccggcag ggtttgggtt accgccgccg ccaagaccgt ggtttccggc
       61 ctaaaattgc ttcaccgcac cggtattttg actgttatta aatattgact taaacaacaa
      121 taagtcaata aataataaca cgtcgaagcg gggtgcggtc aggcattttt cgagcatgac
      181 tcagatttcc ccgatgcgaa cccggatcaa attctgcggc atcacgcgcg ctgccgatgc
      241 gcaggcagcg gccgcgctgg gtgtggacgc gctcggcttc gtgctggtgc cggcaagcaa
      301 gcgctggatc gatcccgtgc gtgcggcgcg catccggcgc gcgctgccgc cgctgatcag
      361 cgtggtggcg ctgttcaagg acgccgccgc ggcgcaggtg caggaagcga tcgatgaact
      421 cgatcccgac ctgctccagt tccacggcga tgaagatgcc gattactgcg ccagtttcgg
      481 caaaccgtat ttgaaggcgg tgagcatgca gcagccgcgt gcgctcgtgc gtgcagcgcg
      541 cgagcaccag catgcggctg cgctgctgct cgacagccac gcgcccggtg gcatgggcgg
      601 cagcggccag atcttcgact ggaaagcggt gccaaaggtg aaacagccgc tgatgctggc
      661 gggcggcctc acgccggcca atgtcggcgc cgcggtgcgc gcgctgcgcc tctgggcggt
      721 ggacgtgtcc agcggcgtcg aaagcaagcc cggcatcaag gacacagcta aaatgcgcgc
      781 ctttgtccac gccgtacagc gcgccgacgc gcggagccga tgaactacca ctaccccgac
      841 agccgcggac actttggtcc ctatggcggc cagttcgtgg cggaaacgct gatggagccg
      901 ctggcgcggc tcaccgaagc ctacgaaaag ctgaagaacg atccggcctt cctggccgag
      961 ctcgacgatg acctgcgcca ctatgtcggc cgcccatcgc cgctctatca cgcgcagcgc
     1021 ctgtcgcaga aatggggcgg cgcgcaggtg tggctcaagc gcgaagacct caaccacacc
     1081 ggcgcgcaca agatcaacaa caccgtgggc caggcgctgc tggccaagca cctgggcaag
     1141 acgcgcatca tcgccgagac cggcgccggc cagcacggcg tggcctctgc cacgatcgcc
     1201 gcgcgcctgg gtctcaagtg cgtggtgtac atgggcgccg aggacatcca gcgccagtcc
     1261 ccgaacgtgt accgcatgaa gctgctcggc gccgaggtgg tgccagtgca cagcggcacc
     1321 aggacgctga aggatgcgct caatgaggcg ctgcgcgact gggtgaccaa tgtcgacgac
     1381 accttctacg tgatcggcac cgtggccggt ccgcatccgt acccgatgct ggtgcgcgat
     1441 ttccagagcg tgatcgggcg cgaggtgatc cagcaggcgc atacgcagat cggccggctg
     1501 ccgcaggcgc tggtggcgtg cgtgggtggc ggctccaacg caattggact tttttacccg
     1561 ttcatcgagc acgacgaggt gaagatgtac ggcgtggaag cgggcgggcg cggcgtcggc
     1621 acgcaggacc atgcggcacc gctgtcggcg ggcaagcccg gcatcctgca cggcaaccgc
     1681 acctacgtga tggccgatgc caacggccag atcatgccga cgcactcgat ctccgccggg
     1741 ctcgattatc ccggtgtcgg tcccgagcat tcgtggctca aggacctgga ccgcgtgatg
     1801 tataccgcgg tgaatgacga cgacgcgctg gccgcattcc acgaactgac gcgcgtggaa
     1861 ggcatcatcc ccgcgctgga gtcgtcgcat gccatcgctt acgcaaagga actcgcggcg
     1921 aagatgccgc aagaccagca catcgtggtg aacctgtc
//
LOCUS       DASZZC010000421         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_612747,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000421 DASZZC010000000
VERSION     DASZZC010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612747"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2024
                     /locus_tag="VHE37_08930"
     CDS             <1..2024
                     /locus_tag="VHE37_08930"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HVT35696.1"
                     /translation="AKSGTTVITRNEGVAASSASLIFMAGDQREMFANTLLMIHAPWQ
                     MAMGNSADLREMADVLDTYAQAMATSYARASGRSVADELAQLTDGKDHWFSAADALTA
                     HYATQVTDAEPPQEESDAQGMPRHQPVVGALTRYRSAPATACASYQPHLRAALKTARA
                     TVPPAAPAASHPRSVSMNLKQIARALGIKIAANATDDQIRASIATKLGLAAGATDDQI
                     SAALESYDPPTQAEPAAQLNAQQQREQEITAMFAVALAHQPENRELQQMRACALIGDA
                     PVATVRKDLLAKLAAGEPAGGTYVPSVQAGADQRDKRRAAAVNWLLVRGTIAKRDSEA
                     VRDLGANPFKGMSISDMARACLEDIGFKVAGRNRHDLISAAITQSTSDFPNIFENALH
                     KTLLAGFTVAPTTWDKICRIGTLTDFRPHIRYRASSIGNLQVRLENGEYKTIALNDAE
                     RETIQAQARGGIINVSREMLVNDDMGVFTDLTMQLGRSASRTREAAFYTALTSNGGLG
                     PTMGDGKTLYHADHGNISAAAGVPSVAAFDADRVKMGSQKDPGGNDFIDVRPSIWLGP
                     LSLGGDVRVINSAQYDPDTANKLQKPNKARGIYDLVIDTPRLTGAPWYSFADPQAEPV
                     FEFGFLDGQQQPQLAMEESFTQHGMKWRVVDEWGVAAIGWRGSVRNAGA"
     gene            2091..2438
                     /locus_tag="VHE37_08935"
     CDS             2091..2438
                     /locus_tag="VHE37_08935"
                     /inference="COORDINATES: protein motif:HMM:NF021473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HVT35697.1"
                     /translation="MAKTHVQPGKVIDVTVDDDYSSGDLIKVGDLCGVCLGDIADGDT
                     GSVQIEEVFQVKKKTSDTITAGKQLYLDADNKRVTISDNDGASVPTAFIKAGKAIAAA
                     SNSDTTVLLKLNV"
     gene            2454..2798
                     /locus_tag="VHE37_08940"
     CDS             2454..2798
                     /locus_tag="VHE37_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35698.1"
                     /translation="MSFGEVAALSAAALLEELGEPAAFTPAGGSPRDTNIVLNETAHD
                     VGLPLPGQGMSEPQLTAELLRADVGVPKRGDAFYFTDAAAPGYAGRRFKVDAVIEVDP
                     FIVKVAAQELSA"
BASE COUNT          534 a         1068 c          893 g          391 t
ORIGIN      
        1 ccgccaagtc cggcaccacc gtcatcacgc gcaacgaggg cgtcgccgcg agcagcgcca
       61 gcctcatctt catggctggc gaccagcgcg agatgttcgc caacacgctg ctgatgatcc
      121 acgcaccctg gcagatggcc atgggcaaca gcgcagacct gcgcgagatg gccgacgtgc
      181 tggacaccta tgcccaggcc atggccacca gctacgcgcg cgcatccggc cgcagcgtcg
      241 ccgacgagct cgcgcagctc accgacggca aggaccactg gttctccgcc gccgatgcgc
      301 tcaccgcgca ctatgccacc caggtcaccg acgccgaacc gccgcaggaa gaatccgacg
      361 cgcaaggcat gccccggcac cagccggtgg tcggcgctct cactcgctac cgcagcgcgc
      421 cggccaccgc ctgcgccagc taccagcccc atttgcgcgc ggcgctcaag actgcgcgcg
      481 caaccgttcc ccccgcggca cccgccgcat ctcacccacg gagtgtttcc atgaacctga
      541 agcagatcgc gcgggcgctc ggcatcaaga ttgccgccaa cgccaccgat gaccagatcc
      601 gcgcctccat cgccaccaag ctgggcttgg ccgccggcgc caccgatgac cagatttccg
      661 cagcgctgga gtcatatgac ccgcccacgc aggcggagcc ggcagcgcag ctcaacgcgc
      721 agcagcagcg cgagcaggaa atcaccgcaa tgtttgcggt cgcgctggca caccagcccg
      781 aaaaccgcga gctgcagcag atgcgcgcct gcgcgctgat cggcgatgcg cccgtggcca
      841 ccgtgcgcaa ggatctgctg gccaagctcg ccgccggcga gccggccggc ggcacgtacg
      901 tgccgagcgt gcaggccgga gcggaccagc gcgacaagcg ccgcgccgcc gccgtcaact
      961 ggctgctggt gcgcggcacc atcgccaagc gcgacagcga ggcggtgcgc gacctcggcg
     1021 ccaatccgtt caagggcatg tccatctccg acatggcgcg cgcctgcctg gaagacatcg
     1081 gcttcaaggt cgccggccgc aaccgccatg acctgatttc cgccgccatc acccagagca
     1141 ccagcgactt cccgaacatc ttcgagaacg cgctgcacaa gacgctgctc gcgggcttca
     1201 ccgtcgcgcc caccacctgg gacaagatct gccgcatcgg cacgctcacg gacttccgtc
     1261 cgcacattcg ctaccgcgcc tccagcatcg gcaacctgca ggtgcgcctg gagaacggcg
     1321 agtacaagac catcgcgctg aacgacgccg agcgcgaaac catccaggcg caggcgcgcg
     1381 gcggcatcat caacgtctcg cgcgaaatgc tggtgaacga cgacatgggc gtcttcaccg
     1441 acctgaccat gcagctcggc cgctcggcca gccgcacgcg cgaagcggcc ttctacaccg
     1501 cgctcacctc caacggtggc ctcggcccca cgatgggcga cggcaagacg ctctaccacg
     1561 ctgatcacgg caacatctcg gccgctgcgg gcgtgccgag cgttgccgcc ttcgacgcgg
     1621 accgcgtgaa gatgggctcg cagaaagacc cgggcggcaa tgacttcatc gacgtccgtc
     1681 cgtccatctg gctcgggcct ctgtccctgg gcggcgacgt gcgcgtcatc aactccgcgc
     1741 agtacgaccc ggacaccgcc aacaagctgc agaagccgaa caaggcccgc ggcatctacg
     1801 acctggtgat cgacacgccg cgcctcaccg gcgccccctg gtattccttc gcggatccgc
     1861 aggcggagcc ggtgttcgag ttcggcttcc tcgatggcca gcagcagccg caactggcca
     1921 tggaggaatc gttcacccag cacggcatga agtggcgcgt ggtggacgag tggggcgtcg
     1981 ccgccatcgg ctggcgcggc tccgtccgca acgccggcgc gtaacgcatc acccaggcgg
     2041 ggccggtccg catgacggcc ccaccaccca caccaagagg atttcgagtc atggcaaaaa
     2101 cccacgtgca gcccggcaag gtgatcgacg tcaccgtcga tgacgactac agctccggtg
     2161 atctgatcaa ggtcggtgac ctgtgcggcg tctgcctcgg cgatatcgcc gacggcgaca
     2221 ccggcagcgt ccagatcgag gaagtgttcc aggtcaagaa aaagacctcg gacaccatca
     2281 cggccggcaa gcagctgtat ctcgacgccg acaacaagcg cgtcaccatc agcgacaacg
     2341 atggcgcctc ggtgcctacc gccttcatca aggcgggcaa ggccatcgcg gcggcaagca
     2401 acagcgacac cacggtgctg ctgaagctca acgtctgatc cagccgtcac gccatgtcgt
     2461 ttggtgaagt tgccgccctg agcgccgccg cgctcctcga agaactcggg gagccggcgg
     2521 cgttcacccc ggctggcggg tcgccccgcg acaccaacat cgtgctcaac gaaaccgcgc
     2581 atgacgtcgg cctgccgctg ccgggacagg gcatgtccga gccgcagctc accgccgagt
     2641 tgctgcgcgc cgatgtgggc gtgccaaagc gcggcgacgc gttctacttc accgatgctg
     2701 cggcgccggg ctatgcgggc cgtcgcttca aggtggatgc ggtcatcgag gttgacccgt
     2761 tcatcgtcaa ggtggcggcg caggagctca gcgcatgagc ctcaccatcg aaacgcccaa
     2821 ccacggcatc gtcctgcgcg ccctgctgga tgcgccaggg tatgtcgcgc ccgggcagcg
     2881 gcgcgc
//
LOCUS       DASZZC010000422         5171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1254019,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000422 DASZZC010000000
VERSION     DASZZC010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254019"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VHE37_08945"
     CDS             complement(<1..462)
                     /locus_tag="VHE37_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HVT35699.1"
                     /translation="MLLHTRAVKKPQPPRVEPIPFLTLPNWIKAAAACGFNIEPIFRR
                     FGIATDLVHLESVSVHPATLEQVMEACIAASREQHFPFVLGERFAFDYIPDIETFLAT
                     ATTLREATRVFDWVRTLINPMIDFRVVERGRHALLVIDIRSADGRAAQPYFI"
     gene            complement(467..889)
                     /locus_tag="VHE37_08950"
     CDS             complement(467..889)
                     /locus_tag="VHE37_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVT35700.1"
                     /translation="MNQGTILEPMTALALWTLGVLTLIPVARFRAGRAGLVRTSDFRF
                     GESANVPPHVSIPNRNLMNLLELPVLFYALCLALYATHLADHTALVLAWGYVVLRMMH
                     SLIHLSYNRVVHRLSVFAVSNLVLTAMWLRFLWVLPST"
     gene            complement(914..1345)
                     /locus_tag="VHE37_08955"
     CDS             complement(914..1345)
                     /locus_tag="VHE37_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HVT35701.1"
                     /translation="MTLKVLYTAHATATGGRNGHTASSDGIVSVDLSVPKAMGGPGKP
                     NTTTPEDLFGAGYAACFGSAVDFMAKQLKLPVTGVKVEAAVGIGARSEGGFGLKVDLT
                     TTVSGLGQAEAEKLVAKAHEVCPYSNATRNNIEVTLKTIGA"
     gene            complement(1398..1724)
                     /locus_tag="VHE37_08960"
     CDS             complement(1398..1724)
                     /locus_tag="VHE37_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HVT35702.1"
                     /translation="MLVTTCNDVPGRRIAQTLGVVRGLVVRSPNVVSSIAGGLRSLLG
                     GNIAEWERTCEAGREEAYARMVKHAQQMGADAIVAMRYDATEFIQGSTEVLAYGTAVK
                     LAGPGA"
     gene            complement(1735..2961)
                     /gene="odhB"
                     /locus_tag="VHE37_08965"
     CDS             complement(1735..2961)
                     /gene="odhB"
                     /locus_tag="VHE37_08965"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HVT35703.1"
                     /translation="MSIEIKVPNLPESVSSGTIATWHVKPGDAVKREQNLVDIETDKV
                     VLEVPAVADGVMGEIKVPAGATVGSGDVLGSIQEGGAAAAPKPATKSEAAPARAAAPA
                     AMASASDDGQSPAVRKLLSELGLSAAQISGSGKGGRLTVEDVKAYADRQKAAPVPAPR
                     ASASAPAPAAPARAGAREEQRVPMTRIRARIAERLVEAQSTAAMLTTFNEVNLHAVNE
                     VRARYKDQFEKTHGVKLGFMSFFVRAAVEALKKYPVVNASVDGQDIIYHGYYDIGIAV
                     SSPRGLVVPILRDADQLSFAGVEKAIADFGARAKDNKLTMEDLTGGTFSITNGGIFGS
                     MMSTPILNPPQSAILGMHGITERPVVVNGEIVIRPMMYLAMTYDHRIIDGREAVLALR
                     TIKECLEDPAKMLLQL"
     gene            complement(3003..>5171)
                     /locus_tag="VHE37_08970"
     CDS             complement(3003..>5171)
                     /locus_tag="VHE37_08970"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HVT35704.1"
                     /translation="KRFSLEGGESMIPALDEMLRACAARDVEEVVIGMAHRGRLNVLV
                     NILGKSPKDLFAEFEGKYSTDSLSRAGDVKYHMGFSTDIEVDGKRLHMVLAFNPSHLE
                     IVNPVVEGSVKARQTRRLDEQGQKVVPILIHGDAAFAGQGVVMETLQLSESKGYDTGG
                     TLHVIVNNQIGFTTPNPIEANPGHEARSSRYCTDLAKMLEAPVFHVNGDDPEAVVFIT
                     QIAMDYRNEFHKDVIVDICCYRRLGHNEADEPAVTSPVMYDAIKKHPTTLALYVRRLE
                     EEGAIAKGEAEALVKAYRDGLDKGENIARTTLGLVGNKHTVNWSKYKQGEWDTPADTS
                     VTRDQLQALSARLLKLPDGFTLHPRVQKIFEDRARMAAGELPMDWGFAEIMAYGSLAN
                     EGFNVRLSGQDSRRGTFFHRHATVHDVKTGERYTPLRHLAKEKYRFVVNDTLLSEVGV
                     LGFEYGYSTTDPDSLVIWEAQFGDFANGAQVLFDQFIASGFAKWGRLCGLTVFLPHGY
                     EGQGPEHSSARLERYLQLCAENNQQVCVPSTPAQMFHMLRRQMKRSLKLPLIVMTPKS
                     LLRHPLSVSSLDDLVKGRFQPVIQETEKLDAGKVKRIVFCSGKVYFDLVQARQKENLS
                     DVAIVRIEQLYPFPREDFERTLAMYPGAKQICWCQEEPENQGAWYQIKHRLGAYIGPQ
                     HQLMYATRKGSATTAVGYLKVHQKEQEEVVREGLSGGKKV"
BASE COUNT          815 a         1714 c         1687 g          955 t
ORIGIN      
        1 gatgaagtac ggctgcgcgg cgcggccgtc ggccgacctg atgtcgatca ccagcagcgc
       61 gtggcggccg cgctcgacca cgcggaaatc gatcatcggg ttgatcagtg tgcgcaccca
      121 gtcgaacaca cgcgtagcct cgcgcagcgt ggtggcggtg gcgaggaagg tctcgatgtc
      181 cggaatgtag tcgaaggcga agcgctctcc gagcacaaag gggaaatgct gctcgcgcga
      241 ggcggcgatg cacgcttcca tcacctgctc cagcgtggcc gggtgcacgc tcacgctctc
      301 caggtgcacc aggtcggtag cgatgccgaa cctgcggaag atcggctcga tgttgaagcc
      361 gcaggcggcg gccgccttga tccagttggg cagcgtgaga aacgggatcg gctcgacgcg
      421 cggcggctgc ggttttttca ccgcgcgagt atgcagcagc actgcttcag gtggacggca
      481 gcacccagag gaagcgcagc cacatggccg tgagcaccag gttggaaacc gcgaacacgc
      541 tgagccggtg caccacgcgg ttgtacgaca ggtgtatcag gctgtgcatc atgcgcagca
      601 ccacgtagcc ccaggccagc accagcgccg tgtggtcggc caggtgcgtc gcgtacagcg
      661 ccaggcacag cgcgtagaac agcaccggca actcgagcag gttcatcagg ttgcgattgg
      721 ggatgctgac gtgcggcggc acgttggccg attcgccgaa cctgaaatcg ctggtgcgca
      781 ccagccccgc gcggccggca cggaagcgcg ccaccgggat cagcgtcagc acgcccagcg
      841 tccacagcgc cagcgccgtc atcggctcga gtatcgtccc ctggttcatc gccgcccccg
      901 gcgcgagcgt gttttatgcg ccgatggttt tcaacgtgac ttcgatgttg ttgcgcgtgg
      961 cattggaata cgggcacacc tcgtgcgcct tggccaccag cttctcggct tccgcctggc
     1021 ccaggccgct gaccgtggtg gtcagatcaa ccttgaggcc gaagcctcct tcgctgcgcg
     1081 cgccgatgcc gaccgccgcc tccaccttca cgccggtcac cggcagcttc agctgcttgg
     1141 ccatgaaatc caccgcgctg ccaaagcacg cggcgtagcc ggcgccgaac aggtcttccg
     1201 gcgtggtggt gttgggcttg cccggaccgc ccatggcctt gggcaccgac aggtccaccg
     1261 agacgatgcc gtcggacgaa gcggtgtggc cgttgcggcc gccggtggcg gtggcgtggg
     1321 cggtgtagag aaccttgagt gtcatgactt gctccttgca gtgaagcgcg cctggcgcgc
     1381 gatgacaaca accggcgtca tgcgccggga ccggccagct tcaccgcggt gccgtaagcc
     1441 agcacctcgg tggaaccctg gatgaattcg gtggcgtcgt agcgcatggc gacgatcgca
     1501 tcggcgccca tctgctgcgc gtgcttgacc atgcgtgcgt aggcttcctc gcggccagcc
     1561 tcgcaggtgc gttcccactc ggcaatgttg ccgcccagca gggagcgcaa accgccggcg
     1621 atgctgctga ccacgttggg cgagcgcacc accagcccgc gcaccacgcc cagggtctgc
     1681 gcgatgcgcc gcccgggtac gtcgttgcag gtggtcacca gcatggaatg ccgctcagag
     1741 ctgcagcagc atcttggccg gatcttccag gcattccttg atcgtgcgca gcgccagcac
     1801 ggcctcgcgg ccgtcgatga tgcggtggtc gtaggtcatg gccaggtaca tcatcggcct
     1861 gatcacgatt tcgccgttga ccaccaccgg gcgctcggtg atgccgtgca tgccaagaat
     1921 cgcgctctgc ggcggattca ggatcggcgt cgacatcatc gagccgaaga tgccgccgtt
     1981 ggtgatgctg aaggtgccgc cggtgagatc ttccatggtc agcttgttgt ccttggcgcg
     2041 cgcgccgaaa tcggcgatgg ccttttccac accggcgaac gacagctggt cggcatcgcg
     2101 caggatcggc accaccaggc cgcgcggcga cgacacggcg atgccgatgt cgtaatagcc
     2161 gtggtagatg atgtcctgtc cgtcgaccga cgcgttgacc accgggtact tcttcagcgc
     2221 ttcgaccgcg gcgcgcacga agaagctcat gaaaccgagc ttgacgccgt gcgtcttctc
     2281 gaactggtcc ttgtagcgcg cgcgcacctc gttgaccgcg tgcaggttca cctcgttgaa
     2341 agtggtgagc atggcggcgg tcgactgcgc ctcgactagc cgctcggcga tgcgcgcgcg
     2401 gatgcgtgtc atcggcacgc gctgctcctc gcgtgcgccg gcacgcgcgg gcgccgccgg
     2461 agccggcgcg gaagcgctgg ccctgggcgc cggtacaggc gccgccttct gccggtcggc
     2521 ataggccttc acgtcttcca ccgtgagccg gccgcccttg ccgctgccgc tgatctgcgc
     2581 agcggacagt ccgagttccg agagcaattt gcgcacggcc gggctctggc catcgtcgct
     2641 cgcgctggcc atcgcggccg gcgccgccgc gcgggccggc gctgcttccg atttcgtagc
     2701 aggcttgggt gcagcagccg cgccgccttc ctggatgctg ccgagcacgt cgccggaacc
     2761 cactgtggca ccggccggca ccttgatctc gcccatcacg ccgtcagcca ccgccggcac
     2821 ttccagcacc accttgtcgg tttcgatgtc gaccaggttc tgctcgcgct tgaccgcgtc
     2881 gcccggcttc acatgccagg tggcgatggt gcccgacgaa acggactcgg gaagattggg
     2941 gaccttgatt tcgatgctca tgtcgattcc ttggattcgt tcttgtcgtg atcaacccgc
     3001 gatcaaacct tcttgccgcc ggacagcccc tcgcgcacca cttcttcctg ttccttctga
     3061 tgcaccttga gatagcccac tgcggtggtc gcgctgccct tgcgcgtggc gtacatcagc
     3121 tggtgctgcg gtccgatgta ggcgcccagg cgatgcttga tctggtacca ggcgccctgg
     3181 ttctccggct cttcctggca ccagcagatc tgctttgcgc ccggatacat cgccagcgtc
     3241 ctttcgaaat cctcgcgcgg gaacgggtac agctgctcga tgcgcacgat ggcgacatcg
     3301 gataggttct ctttctgccg cgcctgcacc aggtcgaagt agaccttgcc cgagcagaac
     3361 acgatgcgct tcaccttgcc cgcatcgagc ttctcggttt cctggatcac cggctggaag
     3421 cggcccttca ccaggtcatc gagcgaagac accgacagcg ggtggcgcag cagtgacttg
     3481 ggagtcatca cgatcagcgg cagcttgagc gagcgcttca tctgccggcg cagcatgtgg
     3541 aacatctgcg cgggcgtcga cggcacgcat acctgctggt tattttccgc gcacagctgc
     3601 aggtagcgct cgagccgcgc cgaggaatgc tccggaccct gcccctcata gccatgcggc
     3661 aggaacacgg tgagcccgca gagccggccc cacttggcaa agccgctggc gatgaattgg
     3721 tcgaacagca cctgcgcgcc gttggcgaag tcgccgaact gcgcttccca gatcaccagc
     3781 gaatccgggt cggtggtgga gtagccgtac tcgaagccga gcacgcccac ttccgagagc
     3841 agcgtgtcgt tgaccacgaa gcggtatttt tccttggcca ggtgacgcag cggcgtgtag
     3901 cgctcaccgg tcttcacatc gtgcacggtg gcatggcgat ggaagaaggt tccgcggcgc
     3961 gaatcctgtc cggacaggcg cacgttgaag ccttcgttgg ccagcgagcc gtaggccatg
     4021 atctcggcga agccccagtc catcggcagc tcgcccgcgg ccatgcgcgc gcggtcctcg
     4081 aagatcttct gcacgcgggg atgcagggta aagccgtcgg gcaacttgag cagccgcgcg
     4141 gaaagcgcct gcagctggtc gcgcgtcact gaggtgtcgg ccggcgtatc ccattcgccc
     4201 tgcttgtact tgctccagtt caccgtgtgc ttgttgccga ccaggcccag cgtggtgcgc
     4261 gcaatgttct cgcccttgtc caggccgtcg cggtaggcct tgaccagcgc ctcggcctcg
     4321 cccttggcga tggcgccctc ctcttccagc cggcgcacgt acagcgccag cgtggtcggg
     4381 tgtttcttga tggcgtcgta catcaccggc gaggtcaccg ccggctcgtc ggcctcgttg
     4441 tggcccaggc gccggtagca gcagatgtcg acgatgacgt ccttgtggaa ctcgttgcgg
     4501 tagtccatcg cgatctgcgt gatgaacacc acggcttcgg ggtcgtcgcc gttcacgtgg
     4561 aataccggcg cctccagcat cttggccagg tcggtgcagt agcggctgct gcgcgcttca
     4621 tgcccggggt tggcctcgat cgggttgggc gtggtgaagc cgatctggtt gttgacgatg
     4681 acgtgcaggg tgccgccggt gtcgtagccc ttggactccg acagctgcag cgtctccatc
     4741 accacgccct ggccggcgaa cgcggcgtca ccatggatca ggatcggcac cactttctgg
     4801 ccctgctcgt ccaggcgccg ggtctggcgc gccttgaccg agccttccac caccggattg
     4861 acgatttcca ggtgcgaggg gttgaacgcc agcaccatgt gcaggcgctt gccgtccacc
     4921 tcgatgtcgg tggagaaacc catgtggtac ttgacgtcgc cggcgcgcga cagcgagtcg
     4981 gtgctgtact tgccttcgaa ctcggcgaac aaatccttcg gtgatttccc gaggatgttc
     5041 accagcacgt tgaggcgccc gcggtgcgcc atgccgatga ccacttcctc gacgtcgcgc
     5101 gcggcgcagg cgcgcagcat ctcgtcgagc gccggaatca tcgactcgcc gccttccagc
     5161 gagaagcgct t
//
LOCUS       DASZZC010000423         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_39237,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000423 DASZZC010000000
VERSION     DASZZC010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39237"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VHE37_08975"
     CDS             <1..378
                     /locus_tag="VHE37_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HVT35705.1"
                     /translation="EAGPACLRELLAAIRKGRLHSYIPIEYRYVKADDIPLSMFEGRD
                     TCAISVHQYYDLDYHNFFAAVEPIFWKYDGRPHWGKLHTLNHGQLKKLYPKMQDFLEV
                     RAALDPAGKFLNGHLRSVLGVNC"
     gene            389..1651
                     /locus_tag="VHE37_08980"
     CDS             389..1651
                     /locus_tag="VHE37_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HVT35706.1"
                     /translation="MKRRTLLMGLGAAAIVALWKRPHDRGAPYEEYFRTLNEELKRNG
                     PMRPCMVIDLDLLDHNIDALLRSVKAPKHYRVVEKSLPSPGLLDYICKRAQTNRLMSF
                     HQPFINDDAEAFPQADILLGKPMPVRAAEAFYKNLKSPFDPSKQLQWLIDSPQHLQEY
                     HELAKGLGTRLRINIELDVGLHRGGVADDAMLNSLLRTIADNPSELEFAGFMGYDPHA
                     AKMPSFMGTPQELVRKVMAIYQARVDFLRGHYAALWNENLTLNTAGSPTYRLHEDEHL
                     SNDISTGSALLKPTDFDVPTLVEHVPAAFIATPVLKASDGVVIPGLEGLGKAMSWWDA
                     NQQQTYFIYGGNWMAQYVSPAGLQRNDIYGHSSNQEMANGSALTGLKVDDHVFLRPTQ
                     SEAVLLQFGDLVAMRGGKIVDYWAPLKA"
     gene            1715..>2270
                     /locus_tag="VHE37_08985"
     CDS             1715..>2270
                     /locus_tag="VHE37_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVT35707.1"
                     /translation="MRKLALLFPLCLSLLLSGCGYNAMQAQDEGVKAAWGEVINQYQR
                     RADLVPNLVNTVKGYAAQEKDVLLGVTEARSKVGSINVTPDMINDEAKLKQFGQAQGE
                     LSAALGRLLSVTENYPDLKSNANFMALQSQLEGTENRIAIARRDYNEAVQGYNTRIRT
                     FPDAIGAKVFYGAKPKVPYQATTPG"
BASE COUNT          429 a          751 c          738 g          352 t
ORIGIN      
        1 gaagccggcc cggcctgcct gcgggagctg ctggccgcca tccgcaaggg caggctgcac
       61 agctacattc ccatcgagta ccgctacgtc aaggccgacg acatcccgct gtcgatgttc
      121 gagggccgcg acacctgcgc catctcggtt caccagtact acgacctcga ctaccacaac
      181 ttcttcgccg cggtggagcc gatcttctgg aagtacgacg gccgcccgca ctggggcaag
      241 ctgcacacgc tcaaccacgg gcaactgaag aagctctacc cgaagatgca ggatttcctc
      301 gaggtgcgcg cggcgctgga cccggccggc aagttcctca acggccacct gcgctcggtg
      361 ctgggcgtta actgctgatg gagcgcgcat gaaacgccgc acgctgttga tgggactggg
      421 cgcggcggcc atcgtcgcgc tgtggaagcg gccgcacgat cgcggcgcgc cgtacgagga
      481 gtatttccgc acgctcaacg aggagctcaa gcgcaacggg ccgatgcggc cctgcatggt
      541 catcgacctg gacctgctgg atcacaacat cgacgccctg ctgcgctcgg tgaaggcgcc
      601 caagcactac cgcgtggtgg agaagtcgct gccgtcgccg gggctgctcg actacatctg
      661 caagcgtgcg cagaccaacc ggctgatgtc gttccaccag ccattcatca acgacgacgc
      721 cgaggccttc ccgcaggccg acatcctgct gggcaagccg atgccggtgc gcgcggccga
      781 agcgttctac aagaacctga agagtccgtt cgatccgtcg aagcagctgc agtggctgat
      841 cgacagcccg cagcatttgc aggaatacca cgagctggcc aagggcctgg gcacgcggct
      901 gcgcatcaac atcgagctgg atgtgggcct gcaccgcggc ggcgtggccg acgacgccat
      961 gctcaacagc ctgctgcgca ccatcgccga caacccgtcg gagctggaat tcgccggctt
     1021 catgggctac gacccgcacg cggccaagat gcccagcttc atgggcacgc cgcaggaact
     1081 ggtgcgcaag gtgatggcga tctaccaggc gcgcgtcgat ttcctgcgcg ggcactacgc
     1141 agctttgtgg aacgaaaacc tcacgctcaa taccgccggc agcccgacct acaggctgca
     1201 cgaggacgag cacctgtcca atgacatctc caccggctcg gcgctgctca agccgaccga
     1261 cttcgacgtg ccgacgctgg tcgaacacgt gcccgctgcc ttcatcgcca cgccggtgct
     1321 caaggccagc gatggcgtgg tgatcccggg gctcgagggc ctgggcaagg cgatgagctg
     1381 gtgggacgcc aaccagcagc agacctactt catttatggc ggcaactgga tggcgcagta
     1441 cgtgtcgccg gcagggctgc aacgcaacga catctacggc cacagcagca accaggagat
     1501 ggccaatggc tcggcgctca ccgggctgaa ggtggacgac cacgtgttcc tgcggccgac
     1561 gcagagtgag gcggtgctgc tgcagttcgg cgacctggtc gccatgcggg gcggcaagat
     1621 tgtcgattac tgggcgccgc tgaaggcctg accggcttga acgcgcgcgg cgcggccgca
     1681 ttatcggttt gacctggggt taccggaggt agtcatgcgc aagttggccc tgctgttccc
     1741 gctgtgcctg tccctgctgc tgtccggctg cggctacaac gccatgcagg ctcaggatga
     1801 gggcgtgaag gcggcctggg gcgaagtcat caaccagtac cagcggcgcg ccgacctggt
     1861 gcccaacctg gtcaacacgg tgaagggcta cgcggcgcag gagaaggacg tgctgctggg
     1921 cgtgaccgag gcccgctcga aggtcggcag catcaacgtg acgcccgaca tgatcaacga
     1981 cgaggccaag ctgaagcagt tcggccaggc gcagggcgag ctttcggccg cgctcggccg
     2041 gctgctctcg gtcaccgaga attatccgga cctcaagtcc aacgccaact tcatggcgct
     2101 gcaatcgcag ctcgagggca ccgagaaccg catcgcgatc gcgcggcgcg actataacga
     2161 ggcggtgcag ggctataaca cgcgcatccg caccttcccc gacgcgatcg gcgccaaggt
     2221 gttctacggc gccaagccca aggtgcccta tcaggcgacg acgccgggcg
//
LOCUS       DASZZC010000424         6772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_736132,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000424 DASZZC010000000
VERSION     DASZZC010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736132"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..448)
                     /locus_tag="VHE37_08990"
     CDS             complement(32..448)
                     /locus_tag="VHE37_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HVT35708.1"
                     /translation="MITHTQIIAAARGWLGTPFRHQGRVRGVGVDCLGLVIGVARELA
                     LVAPDFDVPPYGREPLNGLLLNGLRGQLVPCAEQPGAVALVRWYREPQHVGILTADGT
                     MVHAWDSAGACVEHRYSAPWRKRTVAYFALPGVTCG"
     gene            complement(445..1308)
                     /locus_tag="VHE37_08995"
     CDS             complement(445..1308)
                     /locus_tag="VHE37_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HVT35709.1"
                     /translation="MTATARRAMCWSIVRRDGTVILGTSLDRDLAVTAAGYEGTYASS
                     LGLSASSLRGNSDLSVDNMEVNGLQADMLVSAADIEAGLFDNAQVVIFQTSWADPSSV
                     KVLRRGVLGQITRTREGAYTAEVRGFSQLLSQNTVNVYQATCRATLGSGDEVPVIQRC
                     GVDLSAFRVSGTITALATADRSFTASALTQAAGYFQRGVVTFTSGDNEGFAREIKRHQ
                     AGGVFDLVEPLPYDLQVGDTFTVTPGCDHVYQRADGSYGDCKTKFDNLVNFRGDPLIP
                     GLDAIVQVGTQ"
     gene            complement(1305..1919)
                     /locus_tag="VHE37_09000"
     CDS             complement(1305..1919)
                     /locus_tag="VHE37_09000"
                     /inference="COORDINATES: protein motif:HMM:NF020901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HVT35710.1"
                     /translation="MDFLDVLLDIGFDYGAQGGPDDFGVTIAMASGGRELRVQTRERP
                     LRQWQLGNRTVDRAYLNKIQNYWHTFRGPLIGFLHKDLNDFLAEDEPLVIDGTATAQL
                     IKTYGGGLGNDYVRQILKPAAGATFTKNGAPLSVASVDIATGIVTFNMPYPTADDEVT
                     WSGEFYVPARFASDRLSAQFQAFREEDKQAIYSLGSLVVQELVL"
     gene            complement(1919..4447)
                     /locus_tag="VHE37_09005"
     CDS             complement(1919..4447)
                     /locus_tag="VHE37_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35711.1"
                     /translation="MSKKTDYDINIGGSAAGLDAAVNEAVKSLGKLREKAVDAGKEIL
                     DPFAAVRTAIAGVVATFSFEKVIEEVIASERAMALLEQRIKSTGREGQVTSAQLAGMA
                     EELANVTTYGKDTIVTAESLLLSFSRIGKDIFPRVLETTADLAQGMGVDLTEATFALG
                     KALNNPIDGLDRLQKMGVIFTGQQKKLVRQMVDVGDIAGAQGVILDELEKRFGGTARA
                     AADTFGGALAQLKNAAGDLVGGDGRGLKGVTADIKDLTATLQAPATREGFATFVGGLV
                     SIIGLLAQATSDTGRFGDLVGYLAAKTGGGVSALDELKHQLKDVDQALGSSFLFKPTK
                     FLFTSEADLKALKAQLEQQIKILEDAESFHGGGSKDTTPPPGDSADLGLADRLRRQAD
                     AIKASGILLQGELKRRREALDAELKNELVSFTTYYQQRGDIERAAIDQQLAEQRNHLA
                     QINQQIQAAGNTGDDTDKLESERSQVLAQITELSRQRADVAVRGARDQKQAEKELSDQ
                     LEEVRNKLLELQGDSAWASKGELEKQYRDLLKRLVAEGDVAGQELVHKLINLQVARSE
                     LDQLDKLISTALANMQAREQSIANQNTTGLLTDREASSQIVELHRQTADSISHLVDKY
                     KGLADASGTDEEKVHAQELENQVHELANTVSDEAKRIHDSLHDAFSTAFEDILNGTSS
                     FKDIFRSLFRNIFEGINKEIAGDLSDSLLKSLKGLFSGASGGGSGGGLFSWLAQFFHE
                     GGLVGQDGKWKGIELDLPRFHSGGVLGLQPDEVPAILQMGEEVLTADDPRHRNNVKKG
                     GWSQPIYVNINGVTDAGSFQRNKGQISADVANMLLTALKRSGKG"
     gene            complement(4634..5092)
                     /locus_tag="VHE37_09010"
     CDS             complement(4634..5092)
                     /locus_tag="VHE37_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35712.1"
                     /translation="MGQLTALEPDPRVERVTIRGRTLDVRPLTVARTPAFLREGGPAL
                     LAIFAGESLQPLFKPATPAGEKLALVSQLLAEHQQALVNAIAVALDESPADLAQLNPA
                     ELVELVAAVLGVNLDFFVRAVAPQLGRLALHLVRIQSRLSSATSSAPPRA"
     gene            complement(5096..5842)
                     /locus_tag="VHE37_09015"
     CDS             complement(5096..5842)
                     /locus_tag="VHE37_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35713.1"
                     /translation="MSEQKYTYVGKGKLAIGPYDDSGPKLHVGNCSKLELSVEQAESS
                     VEDFTSAGGGEYDSIERITGVGASFTLYDLSPLNLARDLRGQTSEYTATPVVNEQHKA
                     WKNGVVDLNKVPDPDEDLVVTPHGSTGAFVEGTDYERTLSGIKILEDGDIEDEDQLDI
                     DYTPLPSTIIEALTQAGKKYKLLFEGLNEANSGKPVIVRGHCVNLAPLASLPLIGTDF
                     ASYDVAGKLLKDLAITGTGLSQYFTVHQAK"
     gene            complement(5852..6337)
                     /locus_tag="VHE37_09020"
     CDS             complement(5852..6337)
                     /locus_tag="VHE37_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35714.1"
                     /translation="MTRVLSMLNVIKQRLLTVNGSGQEGIDLGDRVFIGREDIFAAKA
                     DDTNFPCASILEDDETDVSNADIPDRKVMELPITIVAATVSDPDNPLVAGHKLYGDVA
                     RALFNPGTTATGNDRLNGYAMKFEFTGRQIVPRDDGGKITAVYIDCRATYVLQPGNPD
                     L"
     gene            complement(6334..>6772)
                     /locus_tag="VHE37_09025"
     CDS             complement(6334..>6772)
                     /locus_tag="VHE37_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35715.1"
                     /translation="PLSYVRSRIELREASGPDGPATVKARARETRLDRFRYRQLWTRG
                     KNGRRRAAGIAVQVKRGRPMQTLKGAFLVPLKRGREAGAGGMAIALRPKGSKRAYEVL
                     YSSSVRDLLKDQVENKGLGAEIAAYYEQQIALEAQRSIARARA"
BASE COUNT         1089 a         2134 c         2264 g         1285 t
ORIGIN      
        1 gccgacgacg gtgagtatgg cctggccggc attagccaca ggtcaccccc ggcagggcga
       61 aataggctac ggtgcgcttg cgccacggcg cgctgtagcg gtgctccacg caggcgccgg
      121 cgctgtccca ggcgtggacc atggtgccgt ctgcggtgag gatgccgacg tgctgcggct
      181 cgcggtacca gcgtaccagg gcgacggcgc cgggctgttc cgcgcatggc accagctggc
      241 cgcgcagccc gttcagcagc aggccgttga gcggttcgcg cccgtagggc ggcacgtcga
      301 aatccggcgc gaccagcgcc agctcgcgcg cgacgccgat gaccaggccg aggcagtcca
      361 cgccgacgcc gcgcacgcgg ccctggtgac ggaatggcgt gccgagccag ccgcgcgcgg
      421 ccgcgatgat ctgcgtgtgc gtgatcattg cgtgccgacc tgcacgatgg catcgagccc
      481 ggggatcagc ggatcgccgc ggaagttgac caggttgtcg aatttggtct tgcagtcgcc
      541 gtagctgccg tcggcgcgct ggtagacatg gtcgcagccg ggcgtgacgg tgaaggtgtc
      601 gcccacctgc aggtcatagg gcaagggctc gaccaggtcg aacacgccgc cggcctggtg
      661 ccgctttatt tcgcgtgcga aaccttcgtt gtctccgctg gtgaaggtga ccacgccgcg
      721 ctggaaatag ccggcggctt gcgtgagcgc gctggcggtg aagctgcggt cggccgtggc
      781 caatgcggtg atggtgccgc tgacgcggaa ggcggacagg tcaacgccgc agcgctggat
      841 gaccggcacc tcgtcgccag agccgagcgt ggcgcggcag gtggcctggt agacgttgac
      901 ggtgttctgc gagagcagct gcgagaaccc gcggacttcc gcggtgtagg cgccctctcg
      961 cgtgcgcgtg atctgaccga gcacgccgcg ccgcagcacc ttgacgctgg agggatcggc
     1021 ccagctggtc tggaagatga ctacctgcgc gttgtcgaac aggccggcct cgatgtcggc
     1081 cgcgctcacc agcatgtcgg cctgcagacc gttgacctcc atgttgtcga cggacaggtc
     1141 gctgttgccg cgcaggctgc tggcggaaag gcccagggac gaggcgtagg tgccttcgta
     1201 acctgcggcg gtgacagcga ggtcgcggtc gagggaggtg ccgaggatga cggtgccgtc
     1261 gcgccggacg atgctccagc acatggcacg gcgcgcagtg gcagtcacag caccagctcc
     1321 tgcaccacga ggctgccaag cgagtagatg gcctgcttgt cttcctcgcg gaacgcctgg
     1381 aactgcgcgc tcaggcggtc cgatgcgaag cgcgcgggca cgtagaactc accagaccag
     1441 gtgacctcgt cgtccgccgt cgggtacggc atgttaaagg tgacaatgcc ggtggcgatg
     1501 tccaccgagg caacggacag cggcgcgcca ttcttggtga aggtagcgcc agcagccggc
     1561 ttgaggatct ggcgcacata gtcattgccc aggccgccac cgtaggtctt gatcagctgc
     1621 gcggtggcag tgccatcgat gaccagcggc tcgtcttcgg cgaggaaatc gttcagatcc
     1681 ttatgcagga agccgatcag cggcccacgg aaggtgtgcc agtagttctg gatcttgttg
     1741 aggtaggcac gatcgacggt gcgattgccg agctgccatt ggcgcagcgg gcgctcgcgc
     1801 gtctgcaccc gcagctcgcg cccgccggag gccatggcga tggtgacgcc aaagtcatcc
     1861 gggccaccct gcgcgccata gtcaaaaccg atatcgagca gtacgtcgag gaagtccatt
     1921 accccttccc cgaacgcttc agagcggtga gcagcatgtt ggcgacatcg gccgagatct
     1981 ggcccttgtt gcgctggaag ctgccggcgt cagtgacacc gttgatgttt acgtaaatgg
     2041 gctgagacca tccgcccttt ttcacgttgt tgcgatggcg cggatcgtcc gcagtgagca
     2101 cttcctcgcc catctgcagg atggccggca cctcatcggg ctgcaggccg agcacgccgc
     2161 ctgaatggaa gcgcggcagg tcgagctcga tgcccttcca cttgccgtcc tggccgacga
     2221 gtcccccttc gtggaaaaac tgggcgagcc agctgaagag gccaccacca gatccgccgc
     2281 cgctggctcc gctgaaaaga cccttgagcg atttcagcag gctgtcgctg agatcgccgg
     2341 cgatctcctt gttaatgcct tcgaagatgt tgcggaacag gctgcgaaag atgtccttga
     2401 agctgctggt gccattgagg atgtcttcga aggccgtgct gaaagcgtca tgcagtgaat
     2461 cgtgtatgcg cttggcttca tcgctgaccg tattggcgag ctcatgcacc tggttctcaa
     2521 gctcctgcgc atgaaccttt tcctcgtcgg taccgctggc atcggcgagt cccttgtatt
     2581 tgtcgaccag gtgcgaaatt gaatcggccg tctgccggtg cagctcgacg atctgcgacg
     2641 aggcctcgcg atcagtgagc agcccggtgg tgttctggtt ggcaatgctc tgctcgcgag
     2701 cctgcatgtt ggccagcgca gtgctgatca gcttgtccag ctggtccagc tcggaccgcg
     2761 cgacttgaag gttgatgagc ttgtggacca gctcctgccc tgcgacgtca ccctccgcca
     2821 ccaggcgctt gagcaaatcc cggtactgct tttccagttc tcccttgctg gcccaggccg
     2881 aatcgccctg cagttcgagc agcttgttgc ggacctcttc cagctggtcg gagagttcct
     2941 tttccgcctg tttctgatcg cgcgcgccgc gcaccgcgac gtcggcccgc tggcgtgaca
     3001 gctcggtaat ctgcgcgagt acctgcgacc gctccgactc cagcttgtcg gtgtcgtcgc
     3061 ccgtattgcc ggctgcctgg atctgctgat tgatctgcgc caggtgattg cgctgctcgg
     3121 ccagctgctg atcgatggcc gcgcgctcga tgtcgccgcg ctgctggtag taggtggtga
     3181 acgagaccag ttcgttcttc agctctgcat ccagcgcctc gcggcggcgc ttgagttcac
     3241 cctgcagcag gatgccgctg gccttgatgg catcggcttg gcggcgcaaa cgatcggcga
     3301 gcccgagatc cgcgctgtcg ccaggcggcg gcgtagtgtc cttcgaacct ccgccatgga
     3361 aggactcggc atcctccagg atcttgatct gctgttcgag ctgggccttg agtgccttga
     3421 ggtcagcttc tgatgtgaaa aggaacttgg tcggcttgaa caggaagctg ctgcccaatg
     3481 cctggtcaac atccttgagc tgatgtttca gttcatccag cgccgaaacg ccaccgcccg
     3541 tcttggccgc aaggtagccg accaggtcac caaagcgccc ggtgtcgctg gtggcttgcg
     3601 ccagcagccc gatgatggaa acgagcccgc cgacgaaggt ggcgaaacct tcacgggtcg
     3661 caggcgcctg cagggtggcg gtgaggtcct tgatgtccgc agtgacaccc ttgagaccgc
     3721 gaccgtcgcc gccgacgagg tcacccgccg catttttcaa ctgcgccaag gcgccgccga
     3781 aagtgtctgc ggccgcccgg gcggtgccgc cgaatcgctt ctccagctcg tcgaggatga
     3841 caccctgcgc gcccgcgata tcgccgacgt cgaccatctg gcgcaccagc tttttctgct
     3901 ggccggtgaa gatcacgccc attttctgca gccggtccag gccgtcgatg ggattgttca
     3961 gcgccttgcc cagggcgaac gtggcttccg tcaggtcaac tcccatgcct tgcgcgagat
     4021 cggcagtggt ctccagcacg cgcgggaaaa tgtctttgcc aatgcgcgag aaactgagca
     4081 gcagcgattc ggcagtgacg atggtgtcct tgccgtaggt cgtgacgttc gccaattcct
     4141 ccgccatgcc agcgagctgt gcggaagtga cttgaccctc gcgcccggtg gacttgatgc
     4201 gctgttcgag cagtgccatt gccctttcgc tggcgatgac ttcctcgatc accttctcga
     4261 acgaaaatgt ggcaacgacg ccagcgattg ccgtgcgaac ggcggcgaac gggtcaagga
     4321 tttccttgcc cgcatcgacg gccttctcgc gcaacttgcc caggctcttg acggcctcgt
     4381 tgacggcagc atcaaggccg gctgcactgc caccgatatt gatgtcgtag tcagttttct
     4441 tgctcatcga gcgcgttcag gagagcggaa accgccttgg acttgccgct gaagccaccg
     4501 ctgacgccgg cctgtatgag caggagcagg ccgcgttcct gctgacggcg ttccgcccgg
     4561 tgcaggcaat cccaccgccg gagctggcgg agcgaataac cttcgacatc cgcgcgcgca
     4621 tggccggaag cgatcaggcg cgcggcggcg cgctcgatgt cgcggaggaa agccgcgact
     4681 ggatccggac caagtgcagc gccaggcgcc ccagttgcgg ggccacggcc ctgacgaaaa
     4741 aatcgaggtt cacccccaaa accgctgcga ccagctcaac aagctccgcc gggttgagct
     4801 gggcaagatc ggccgggctc tcgtccagtg ccaccgcgat ggcgttgacc agcgcctgct
     4861 ggtgttccgc caacagctgg ctgaccagcg ccagtttctc ccccgccggc gttgcgggct
     4921 tgaacagggg ctgcagactt tcgcccgcga aaatggccag cagggccgga ccgccctcgc
     4981 gcaggaatgc gggtgtgcgc gccaccgtga gcgggcgcac gtcgagcgtg cggccgcgga
     5041 tggtgacgcg ctccacgcgc ggatccggtt cgagggcggt gagctggccc atggcttact
     5101 tggcctgatg caccgtgaag tactgcgaca ggccggtgcc ggtgatggcc aggtccttga
     5161 gcagcttgcc ggcgacgtcg tacgaggcga agtcggtgcc gatcagcggg aggctggcca
     5221 gcggcgccag gttgacgcag tggccgcgga cgatgaccgg cttgccgctg ttggcctcgt
     5281 tgaggccttc gaacagcagc ttgtacttct tgcccgcctg ggtgagcgcc tcgatgatgg
     5341 tgctcggcag cggcgtgtaa tcgatgtcga gctgatcctc gtcttcgatg tcgccgtctt
     5401 ccaggatctt gatgccggag agcgtgcgct cgtagtcggt gccttcgaca aatgcgccgg
     5461 tgctgccatg cggggtgacc accagatcct cgtcggggtc cggcaccttg ttgaggtcga
     5521 cgacgccgtt cttccaggcc ttgtgctgct cgttgaccac cggcgtggcg gtgtattcgc
     5581 tggtctggcc gcgcaggtcg cgcgcgagat tcagcggcga caggtcatac agcgtgaacg
     5641 atgcgccgac gccggtgatg cgttcgatgc tgtcgtactc gccgccaccg gcgctggtga
     5701 aatcctcgac cgaggactcg gcctgctcca ccgagagctc cagcttggag caattgccca
     5761 cgtgaagctt cgggccggag tcgtcatagg gtccgatggc cagcttgccc ttgccgacat
     5821 aggtgtattt ctgttcgctc attgcgttct cctacaggtc tggattgccc ggctgcagca
     5881 cgtaagtggc gcggcagtcg atgtaaacgg cagtgatctt tccgccgtca tcgcgcggga
     5941 cgatctggcg gccggtgaat tcgaacttca tggcgtaacc gttgaggcgg tcattgccgg
     6001 tggcggttgt gccagggttg aacaacgcgc gcgccacatc gccgtagagc ttgtgaccgg
     6061 cgaccagcgg gttgtcggga tcgctgaccg tggcagcgac gatggtgatg ggcagctcca
     6121 tcaccttgcg gtccgggatg tcggcgttgc tgacatcggt ttcgtcgtct tcaaggatcg
     6181 acgcgcatgg gaagttagtg tcgtcagcct tggcggcgaa aatgtcttcg cgcccgatga
     6241 acacacggtc acccaggtca atgccttcct gccccgaccc gttgacggtg agcaggcgct
     6301 gcttgatgac gttgagcatg ctgagcacgc gggtcatgcg cgcgccctcg caatggaccg
     6361 ctgcgcttcg agcgcaatct gttgctcgta gtaggcggcg atctccgcgc cgagcccctt
     6421 gttctccacc tggtccttga gcaggtcgcg cacgctggag ctgtagagaa cctcgtaggc
     6481 ccgcttggag cccttggggc gcagcgcgat ggccatcccg ccggcgcccg cttcgcggcc
     6541 acgcttgagc ggcacgagga acgctccctt cagggtctgc atggggcgcc cacgcttgac
     6601 ctgaacggcg atgccagcgg cacggcggcg gccgttcttg ccgcgggtcc acagctggcg
     6661 gtagcggaag cgatcaagcc gggtttcgcg cgcgcgggcc ttgaccgtgg cggggccgtc
     6721 cggccccgag gcctcgcgca attcaatgcg cgaccgcacg taggacagcg gc
//
LOCUS       DASZZC010000425         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_613008,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000425 DASZZC010000000
VERSION     DASZZC010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613008"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VHE37_09030"
     CDS             complement(<1..488)
                     /locus_tag="VHE37_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HVT35716.1"
                     /translation="MFERPRSGQRAVLVHLEFPRADFDSDRREFIELASSAGADIVTV
                     IGGVRRVPDAGTYAGSGKVEEIAAAVAGSGAELVIFNHELSPSQERNLEKAIKARVVD
                     RAGLILDIFAQRARSHEGKLQVELAQLHHVATRLVRGWTHLERQRGGAIGLRGPGETQ
                     LEL"
     gene            complement(512..787)
                     /gene="hfq"
                     /locus_tag="VHE37_09035"
     CDS             complement(512..787)
                     /gene="hfq"
                     /locus_tag="VHE37_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HVT35717.1"
                     /translation="MSKGHTLQEPFLNALRKERIPVSIYLVNGIKLQGQIESFDSFVV
                     LLRNSVSQMVYKHAISTVVPARAVRVYEGEGGEGEGGAAPEAPAAGH"
     gene            complement(858..>1672)
                     /gene="miaA"
                     /locus_tag="VHE37_09040"
     CDS             complement(858..>1672)
                     /gene="miaA"
                     /locus_tag="VHE37_09040"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HVT35718.1"
                     /translation="QVYRGMDIGTAKPDAATLARVPHHLVDMLDPRESYSAARFAADA
                     ARIIGEVRARGRLPLLVGGTMLYFRALRHGLSDLPEADAQVRARIAADAQREGWPALH
                     ARLAQRDATTAARLHPNDQQRIQRALEIIELSGQSPSEFYAEPKTPPLALPVLALALN
                     PPARAELHARIERRFHAMMQAGFLDEVRRLHARGDLTPELPSIRSVGYRQLWQHLDGL
                     YALDEAIGRGIAATRQYAKRQLTWLRSEKNLHWLDPTRPDLVARVRNLLGMT"
BASE COUNT          246 a          568 c          583 g          275 t
ORIGIN      
        1 agttccagct gggtttcgcc gggaccgcgc aggccgatgg cgccgccgcg ctgccgctcc
       61 aggtgggtcc agccgcgcac cagccgcgtg gccacgtggt gcagctgcgc cagttccacc
      121 tgcagcttgc cctcgtgcga acgcgcgcgc tgggcgaaga tgtccaggat cagtccggcg
      181 cggtcgacca cgcgcgcctt gatcgccttc tccaggttgc gttcctggct cggcgaaagt
      241 tcatggttga aaatcaccag ttcggcgccg ctgccggcaa cggcggcggc gatctcctcc
      301 accttgccgc tgccggcata ggtgccggcg tcgggcacgc gccgcacgcc gccgatcacc
      361 gtgacgatgt cggcgccggc ggaactggca agctcgatga actcgcgccg gtcgctgtcg
      421 aagtcggcgc gcggaaattc aaggtgaacc aggaccgcgc gctgtccgct gcgcggccgc
      481 tcaaacatgg gtacagcggc gaatcagcag atcaatgccc cgctgccgga gcttccggcg
      541 ccgcgccgcc ttcgccctcg ccgccttcgc cctcgtacac gcgcaccgcg cgcgccggga
      601 ccacggtgga aatggcgtgc ttgtagacca tctggctgac gctgttgcgc aggagcacga
      661 caaaggaatc gaacgattcg atctggccct gcagcttgat gccgttgacc agatagatcg
      721 aaaccgggat gcgttcctta cgcagggcgt tcaggaaggg ttcttgtaat gtgtgacctt
      781 tggacattgt cttgatcctg ttcgagttct aatggccagt gttcccctgg gcttttcagt
      841 ctacagccta gttaggacta tgtcatgccg agcaagttcc tcacccgcgc caccaggtcg
      901 gggcgtgtcg ggtcgagcca gtggagattt ttctccgagc gcagccaggt gagctggcgc
      961 ttggcgtact ggcgcgtggc ggcgatgccg cggccgatcg cctcgtccag cgcgtacagc
     1021 ccgtcgaggt gctgccacaa ctggcggtaa cccacgctgc ggatcgacgg cagctccggc
     1081 gtgagatcgc cgcgcgcgtg cagccgccgc acctcgtcga gaaaaccagc ctgcatcatg
     1141 gcgtggaagc gccgctcgat gcgcgcgtgc agttcggcgc gcgccggcgg attgagcgcc
     1201 agcgccagca ccggcagcgc cagcggcggc gttttcggct cggcgtagaa ctcgctcggc
     1261 gactggccgg acagctcgat gatctccagc gcgcgctgga tgcgctgctg gtcgttggga
     1321 tgcagccgcg ccgcagtggt ggcgtcacgc tgcgccagcc gcgcatgcag cgcgggccag
     1381 ccctcgcgct gcgcgtcggc ggcgatgcgc gcgcgcacct gcgcgtcggc ctccggcaga
     1441 tcggacagcc cgtgccgcag cgcgcggaag tacagcatgg tgccgccgac cagcagcggc
     1501 aggcggccgc gcgcgcgcac ctcgccgatg atgcgcgcgg catccgcggc gaagcgcgcc
     1561 gcggaatagc tctcgcgcgg atcgagcatg tcgaccaggt ggtgcggcac gcgggcgagc
     1621 gtggcggcgt cgggcttggc ggtgccgatg tccatgccgc ggtacacctg cg
//
LOCUS       DASZZC010000426         3863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_39641,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000426 DASZZC010000000
VERSION     DASZZC010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39641"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VHE37_09045"
     CDS             <1..541
                     /locus_tag="VHE37_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT35719.1"
                     /translation="VQNAEANAQASRAQVEFAGAGNRPRVSLDGSADTSDNGEFGYDR
                     LTTWQVLLKLQIPIYQGGLTHAKVAEATARAEQASAVAEDTRRAFAETATREWEMLQA
                     ADEVMKSYQAQVDAAQSALDGVRKELEVGSRTTLDLLNAEREVLAAQVNLLSSRRDRA
                     VTAFSLLAACGELEPDAIP"
     gene            complement(745..1521)
                     /locus_tag="VHE37_09050"
     CDS             complement(745..1521)
                     /locus_tag="VHE37_09050"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HVT35720.1"
                     /translation="MVALATEKVTHVHHWNDTLFSFRTTRDPALRFHNGHFVMIGLPV
                     NGKPLMRAYSIASANYEEHLEFLSIKVQDGPLTSRLQHLKVGDELIVSRKPTGTLILD
                     DLKPGKNLFMLGTGTGLAPFMSLVKDPQTYEMFEKVILVHGVRQVSDLAYHDFLAQDL
                     HDHEFLGEMVKQKFIYYPTATREPFRNNGRLTELLDSDKLTADIGLPPLNPATDRAMI
                     CGSPAMLADTRALLDKRGFVVSPHIGTPGDYVFERAFVDK"
     gene            complement(1611..1952)
                     /locus_tag="VHE37_09055"
     CDS             complement(1611..1952)
                     /locus_tag="VHE37_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35721.1"
                     /translation="MQKHIARIELVKDMIQQAIDRGATSVEQIHQYIADLPFEALEKS
                     GMLDERASALREKKSRTIGVVYDAIRRINREVGMLVSDQLENLEDGHYVSKVLAEKAP
                     AKKRRPRKKLG"
     gene            complement(1964..3169)
                     /locus_tag="VHE37_09060"
     CDS             complement(1964..3169)
                     /locus_tag="VHE37_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35722.1"
                     /translation="MAAQVRGADLRGYSRLVIDATVKITDLVEAMHAGIARPQFLFGK
                     PAPRTDGITGLVYQSVRTISTLVGAGIDSLLELAAPLVDAAQAHDASPYVREGVQALL
                     NGVIGDHLEHSGNPLAIRMSLRRNGISIAPDTRAIVEAIPEANGKLLILAHGLCMNDL
                     EFNWKGHDHGAMLARERGWTPVYLHYNSGRHVSQNGREFADQLEALVRAWPMPLEEIA
                     ILGFSMGGLVARSACHYGAQAQHSWLQHLKRIVFVGTPHHGAPLERGGNHFHQFAASL
                     PFAGPLARLGAIRSAGVTDLRHGNLLDQDWQDCGRFEHHHDRRTPVPLPDGVRCYALA
                     ASTGERAGDLSDTLLGDGIVFLNSALGRHDDPRRALSFDDHCTHFETGHLGMLDSRAV
                     YATIRTWLT"
     gene            complement(3172..>3863)
                     /locus_tag="VHE37_09065"
     CDS             complement(3172..>3863)
                     /locus_tag="VHE37_09065"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HVT35723.1"
                     /translation="LGIDKDVGYGLLRVDDQQRWKWKHAIQPASNALLSLLFQWGVGI
                     HDVDWGGFLKGRVPRDEFRAQMRPFLRKAGRQLFKDYVFFPALALWNAPRVFAGNLAA
                     NMIRNVWSNVIIFCGHFPEGTRVYTQEETRNETRGDWYIRQMAGSANIEGGRAFYILT
                     GHLSHQIEHHLFPDIPAHRYPEMAPRVRALCEKYGLPYNSGSFWKQYGSVLTGLIKRS
                     LPTRAPQPALA"
BASE COUNT          629 a         1227 c         1329 g          678 t
ORIGIN      
        1 ggtgcagaac gccgaggcca acgcgcaggc cagccgcgcg caggtggagt tcgccggcgc
       61 gggcaaccgt ccgcgcgtgt cgctcgacgg cagcgccgac acctccgaca acggcgagtt
      121 cggctacgac cggctgacca cctggcaggt gctgctcaag ctgcagatcc cgatctacca
      181 gggcgggctg acgcatgcca aggtggcgga agcgacggcg cgcgccgagc aggccagcgc
      241 ggtggccgag gatacgcgcc gcgcgtttgc ggaaacagcc acgcgcgaat gggagatgct
      301 gcaggcggcc gacgaggtga tgaagtcgta ccaggcgcag gtcgacgccg cgcagtcggc
      361 gctcgacggc gtgcgcaagg aactggaagt gggctcgcgc accacgctcg acctgctcaa
      421 cgccgagcgc gaagtgctgg cggcgcaggt caacctgctg tcgagccggc gtgaccgcgc
      481 ggtcaccgcc ttcagcctgc tggcggcctg cggcgagctg gagcctgatg caattccctg
      541 aaggccgatc gggaagacaa aaagcggccc gcgggccgct tttttcaggt caccgcaccg
      601 gccctggtga tgttataaaa aattgcctga tattaaaaac agacattaat ttataacaga
      661 caaatcggcg ttgcggtcag gcccggtttc ctggatttcc cagaactgga tccccgcctc
      721 cgcgcggatg acgcgctgcg cgcgtcactt gtcgacgaac gcgcgctcga acacgtagtc
      781 gcccggcgtg ccgatgtgcg gcgagaccac gaagccccgc ttgtccagca gcgcgcgcgt
      841 gtcggccagc atcgccggcg agccgcagat catggcgcgg tcggtggccg ggttgagcgg
      901 cggcaggccg atgtcggcag tgagcttgtc gctgtcgagc agttcggtca ggcggccgtt
      961 gttgcggaac ggctcgcgcg tggcggtcgg gtagtagatg aacttctgct tgaccatctc
     1021 gccgaggaat tcgtggtcgt gcaggtcctg cgcgaggaag tcgtggtacg caaggtcgct
     1081 cacctggcgc acgccgtgga ccaggatcac cttctcgaac atctcgtaag tctgcgggtc
     1141 cttgaccagc gacatgaacg gcgccagccc ggtgccggtg ccgagcatga acaggttctt
     1201 gccgggcttg aggtcgtcga ggatcagcgt gccggtgggc ttgcggctga cgatcagctc
     1261 gtcgccgacc ttcaggtgct gcaggcgcga ggtgagcggg ccgtcctgca ccttgatgct
     1321 gaggaattcc aggtgctctt cgtagttggc cgaggcaatg gagtaggcgc gcatcagcgg
     1381 cttgccgttg accggcaggc cgatcatgac gaaatgccca ttgtgaaaac gcagggcggg
     1441 atcgcgcgtg gtgcggaacg agaacagcgt gtcgttccag tggtggacgt gcgtgacctt
     1501 ctctgtggct aaagcgacca tgttgctgac tctgccggcc tgaaaacggc gcgtattcta
     1561 cgggcggcgg gcggccaaaa aatactcaga actgggtata tgggtagaac ttatcccagc
     1621 ttcttgcgcg ggcgcctctt cttcgccggc gccttctcgg ccagcacctt gctcacgtag
     1681 tggccgtcct ccaggttttc cagctggtcc gacaccagca tgccgacttc gcggttgatg
     1741 cggcggatgg cgtcgtaaac cacgcctatg gtgcgcgact tcttctcgcg cagcgcgctg
     1801 gcgcgctcgt cgagcatgcc ggatttctcc agcgcctcga acggcaggtc ggcgatgtac
     1861 tggtggatct gctccaccga ggtggcgccg cggtcgatgg cctgctggat catgtccttg
     1921 accagttcga tacgcgcgat gtgcttctgc atgggtttcc cgctcatgtc agccaggtcc
     1981 gtattgtggc gtagaccgcg cggctgtcga gcatgcccag gtggccggtc tcgaagtgcg
     2041 tgcagtggtc atcgaaggaa agcgcgcggc gcggatcgtc gtgccggccc agcgcgctgt
     2101 tcaggaagac gatgccgtcg ccgagcaagg tgtcggacag gtcgccggcg cgctcgcccg
     2161 tgctcgcggc cagcgcgtag cagcgcacgc cgtccggcag cggcaccggc gtgcggcggt
     2221 cgtggtgatg ctcgaagcgc ccgcagtcct gccagtcttg gtccagcagg ttgccgtggc
     2281 gcaggtcggt gacgcccgcg ctgcggatgg cgcccaggcg cgccagcggg ccggcgaacg
     2341 gcagcgaggc cgcgaactgg tggaagtgat tgccgccgcg ttccagcggc gccccgtggt
     2401 gcggtgtgcc gacgaagacg atgcgcttca gatgttgcag ccacgagtgc tgtgcctgcg
     2461 cgccgtagtg gcaggcgctg cgcgcgacca gcccacccat cgaaaacccg aggatcgcga
     2521 tttcctccag cggcatcggc cacgcgcgta ccagcgcttc gagctggtcg gcgaactcgc
     2581 ggccgttttg cgacacatgc cgcccgctgt tgtagtgcag gtacaccggc gtccagccgc
     2641 gctcgcgcgc cagcattgcg ccgtgatcgt ggcctttcca gttgaactcc aggtcgttca
     2701 tgcacaggcc atgcgccagg atcagcagct tgccgtttgc ttcgggaatt gcctcgacga
     2761 tcgcgcgcgt gtcgggcgca atggaaattc cattgcgccg caggctcatg cggatcgcga
     2821 gcggattgcc gctgtgttcg aggtgatcgc cgatcacgcc gttgagcaag gcctgcacgc
     2881 cttcgcgcac atagggcgaa gcgtcgtgcg cctgcgcggc atcgaccagc ggcgcagcca
     2941 gttccagcag gctgtcgatc ccggcgccga ccagcgtgct gatggtgcgc acgctttgat
     3001 acaccaggcc ggtgatgccg tcggtgcgcg gcgccggttt gccgaagagg aattgcggcc
     3061 gcgctatgcc ggcgtgcatg gcttccacca ggtcggtgat cttcaccgtg gcgtcgatca
     3121 ccaggcggct gtagccgcgc agatcggcgc cgcgcacctg cgccgccatg ttcacgccag
     3181 cgccggctgc ggcgcgcgcg tgggcagcga gcgcttgatc aggcccgtga gcaccgagcc
     3241 gtactgcttc cagaaactgc cgctgttata gggcaagccg tatttctcgc acagcgcgcg
     3301 cacgcgcggc gccatctcgg gataacggtg cgcgggaatg tccgggaaca ggtgatgctc
     3361 gatctggtgt gacagatgcc cggtgaggat gtagaacgcc cgcccacctt caatgttcgc
     3421 ggagccggcc atctggcgga tgtaccagtc gccgcgcgtt tcattgcgcg tctcttcctg
     3481 cgtatacacg cgagtgcctt ccgggaaatg gccgcagaag atgatcacgt tgctccacac
     3541 gttgcggatc atgttggcgg cgaggttgcc ggcgaacacg cgcggcgcgt tccacagcgc
     3601 cagcgccggg aagaacacgt aatccttgaa cagctgcctg ccggccttgc gcaggaacgg
     3661 gcgcatctgc gcgcggaact cgtcgcgcgg cacgcggccc ttgaggaacc cgccccagtc
     3721 gacgtcgtgg atgcccacgc cccactggaa cagcagcgac agcagcgcgt tgctggccgg
     3781 ctggatcgcg tgcttccact tccagcgctg ctgatcgtcc acccgcagca ggccgtagcc
     3841 gacgtccttg tcgatgccaa gga
//
LOCUS       DASZZC010000427         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_291907,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000427 DASZZC010000000
VERSION     DASZZC010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291907"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1773)
                     /locus_tag="VHE37_09070"
     CDS             complement(61..1773)
                     /locus_tag="VHE37_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HVT35724.1"
                     /translation="MTIAAAATSEEFLGHPKGLYVCFFTEMWERFSFYGMKSLLVLYL
                     VKHHLFSDSDSLAIQGAYGGMVYAIPVIGGLVADRWLGMRKSVVLGGILLCLGHLGMS
                     VEGHAAQVVDGHVQRDEFALLVFYASLALIISGVGFLKPNISTIVGKLYPDGDPRRES
                     GFTLFYAGINVGALFASLICGYLGEAYGWSYGFGAAGIGMLAGLAMFISGQKYLMGHA
                     EPRDAAALAQRVLGPLTLEWSIYLAAVLALPAIFLLMKLGSAVLYLQFLLIGSWLVWL
                     AWYVVERCNRVQREQMLAVVLFTSFCLLFFALYEQTYGSWVLFTDRMLDKDLFPAFVI
                     REGQPLPWSIAPLIFAPLAMGIALRMRAEKTARGLIGITAALGTLIIVRDCVVLPQTA
                     ESLTYLGALFIVLLSPLFAWLWPFLDRRGLNPSKPLKSAIGLAFSGLAFLPLAAANAG
                     VTDGHLGSVWWLVLAYLLIEIGEVSLSPIGLAAVTDLSVPAVVGVMMGAWWLATSFSE
                     QAAAALGKLAALELPADGKIDFTVAVQKYGDLFHQMVWLGLGAAVFALVITPLVRRWM
                     HGVR"
BASE COUNT          395 a          668 c          631 g          274 t
ORIGIN      
        1 cggtgccctc tcccccggcc tgctgctcgg cttgctgatc ctgcgcgcgc gtcggcggcg
       61 ttagcgcacg ccatgcatcc agcgccgcac cagcggcgtg atgaccagcg cgaacaccgc
      121 ggcgcccagg ccgagccaca ccatctggtg aaacaggtcg ccgtacttct gcacggccac
      181 ggtgaagtcg atcttgccgt ctgccggcaa ttccagcgcg gcgagcttgc ccagcgcggc
      241 agcggcctgc tcggagaatg atgtcgccag ccaccacgcg cccatcatca cgccgaccac
      301 cgccggcacc gacaggtcgg tgaccgcggc cagcccgatc ggcgacagcg acacttcgcc
      361 gatctcgatc agcaggtacg cgagcaccag ccaccacacg ctgcccaggt gtccatccgt
      421 cacgccggca ttggccgcgg ccagcggcag gaaggcgagg ccggagaatg cgaggccgat
      481 cgcactcttc agcggcttgc tcggattgag cccgcgccgg tcgaggaacg gccacagcca
      541 ggcgaacagc ggcgacagca gtacgatgaa cagcgcgccg agataagtga gcgactcggc
      601 ggtctgcggc agcaccacgc agtcgcgcac gatgatcagc gtgcccagcg ccgcggtgat
      661 gccgatcagc ccgcgcgcgg ttttttccgc gcgcatgcgc agcgcgatgc ccatggccag
      721 cggcgcgaag atcagcggcg cgatcgacca cggcagcggc tggccttcgc ggatgacgaa
      781 cgcggggaac aggtccttgt cgagcatgcg gtcggtgaac agcacccaag agccgtaggt
      841 ctgctcgtac agcgcgaaga acagcaggca gaagctggtg aacagcacca ccgccagcat
      901 ctgctcgcgc tgcacgcggt tgcagcgttc caccacgtac caggccagcc acaccagcca
      961 gctgccgatg agcaggaact gcaggtacag caccgcgctg cccagcttca tcagcaggaa
     1021 gatggccggc agcgccagca cggcggcgag atagatgctc cattccagcg tcagcgggcc
     1081 gagcacgcgc tgcgcaagcg cagcggcatc gcgcggctcg gcgtggccca tgaggtattt
     1141 ctggccgctg atgaacatgg ccagcccggc gagcatgccg atgccggcag cgccgaagcc
     1201 atagctccag ccgtaggcct cgccgaggta tccgcaaatc agggaggcaa acagcgcgcc
     1261 gacgttgatg ccggcgtaga acagcgtgaa gccggattcg cgccgcggat cgccgtcggg
     1321 atacagcttg ccgacgatgg tggagatgtt cggcttgagg aaacccacgc cgctgatgat
     1381 cagcgcgagc gaggcataga acaccagcaa tgcaaactcg tcgcgttgca cgtgcccatc
     1441 gaccacctgt gccgcatgtc cttccaccga catgccgagg tgccccaggc acagcaggat
     1501 gccgccgagc accacgctct tcctcatgcc cagccagcgg tcggccacca gtccgccgat
     1561 cactggaatc gcgtacacca tgccgccgta cgcgccctgg atggccaggc tgtcgctgtc
     1621 ggaaaacagg tgatgtttca ccaggtacag caccagcagc gacttcatgc cgtagaagga
     1681 aaagcgctcc cacatctcgg tgaagaagca cacgtacagg cctttcggat ggccgaggaa
     1741 ttcttcggag gtcgcggcgg cggcgatggt catggcgtga tcggggttcc ctgttgcgat
     1801 gagtataggg tgagacactg cagcatcagc gcgccgccgg acctgcggtt accatgcgcg
     1861 cgagaccata caagggcact cccatgaaga acttcctgct ggccaccctg ctggcactga
     1921 cggcgacgca aaccatgagc gcggacaaga tcgacaagag cggcaagc
//
LOCUS       DASZZC010000428        14254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_97477,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000428 DASZZC010000000
VERSION     DASZZC010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97477"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VHE37_09075"
     CDS             <1..1068
                     /locus_tag="VHE37_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35725.1"
                     /translation="DQLLAAGLNFRNYGERGGGANPEPASQGANRTLEQFAQVQLHSS
                     PDYPFNGLSACMQASANVDPAPLFEGCIYDSSARTQSRLTGANVAPPGAVSRFALFEA
                     EFTPLVTAGQWYPFTYLIQFNDHGQGSTPNNTTEAAQAADNDLALGQLVDLVSHSPIW
                     PQSAIFVMEDDSQDGGDHVDSHRMPAYVISPWAKQGADAAHAPVIAQRYDQLAMLRTI
                     QLILGLPSASLLHSLASPMYDVFIDPMQAPDLTPYTAILPERSLVEQVGKTAASMAFA
                     RNAPGLYALSQKLPWHRMDAVPQEIADRVHYANLWGDDRHYPGPGPNASAEEHERAVD
                     LLRVLKRGGDARRLLQTADDD"
     gene            1126..2277
                     /locus_tag="VHE37_09080"
     CDS             1126..2277
                     /locus_tag="VHE37_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HVT35726.1"
                     /translation="MNTAGNTPPNHSLWDLVRYALRLGTTGFGGPVALVGYMYRDLVE
                     QRKWISEADYKEGLTLAQLMPGPLAAQLAMYLGYVHYGVLGASLVGVAFVVPSFLMVL
                     ALGWLYVGYGGLNWMQAVFYGVGAAVIGIIAMSAYRLTTRNIGRDPLLWGIFLVSAAI
                     TAITASEMIGVFLAGGILVWLVRAPPKLGGGSVSAVAALAPAVGAGKGPLLMQIFLFF
                     AKAGAVVFGSGLAIVPFLYGGVVHEHQWLSEQQFVDAVAVAMITPGPVVITVGFIGYL
                     VAGFPGAAVAALATFLPCYLFTVIPAPYFKKHGKRPAIVAFVDGATAAAVGTIAGSVV
                     VLGQRSITDLPTLAIALAALGVMWRYKKVQEPVIVLAAAALGLLIRGLG"
     gene            complement(2442..2960)
                     /locus_tag="VHE37_09085"
     CDS             complement(2442..2960)
                     /locus_tag="VHE37_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HVT35727.1"
                     /translation="MRAAHSCGCAAQYASFCNSHEISQQIDVQSRAHPANLAHKSASS
                     AALAAARHDAKDRARLEQTAAAVEQAWRSDDRRVQLTTDLAFHHAIAEATHNPVFSYL
                     MGSLHRLLLDHMQLSQAGTEPKSGITDQVRAQHRELLRMILARDGKGATRAASRHIEF
                     VRVRLNHLPPRA"
     gene            2862..3719
                     /locus_tag="VHE37_09090"
     CDS             2862..3719
                     /locus_tag="VHE37_09090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT35728.1"
                     /translation="MSTRLNIDLLRNFVAIAEARVLSRAAARVGRTQAALSQQVKRLE
                     AEVQQTLMIRGGRGITLTAQGERLFLHAQNILRTHDEAVGELLGVTLSGTLRMGCPDD
                     YALAFLPSLLRGYSNLHPQVMIEVACAPTPRLLERIKNQELDIAIVSETDSSQRDQLL
                     RREQFVWVGVKGGDVCNRDPLPLALSDIDTMVHQAATSRLERAGRKYRIAYASDGIAG
                     LTAVVRSGQAIAVLTKSAVPPDLYILPPSSGLPKLPSIGITVKTARRNSSPLLRSFEA
                     HVRSVLPTL"
     gene            3798..5618
                     /locus_tag="VHE37_09095"
     CDS             3798..5618
                     /locus_tag="VHE37_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVT35729.1"
                     /translation="MSDIPDDFKARILNPGYVERIEMIPMRDGVKLKTYIYIPAGAKG
                     AAILLMRTPYNASTLKAKIAGDRYLEAGYIRVMQDVRGKYGSEGEYVVTRPVRGALNA
                     SDADHPTDTWDTIDWLVQNLRESNGRVAIFGNSYVGFTSAMALLDPHPALRAAVPVGP
                     LIDAWMGDDWFHYGAFRQMMLGFIHKQTAQRGEGVVTPSVTHDKYEEFLRAGSAADYI
                     REHGLDRLPFAARLMAHPAYDAYWQAQALDKLLAARPSRVPTMWTQGLWDQEDMYGAN
                     HAWRALKAAGYEANNWLVLGPWNHVQAWRTAASLGPMQWHADTAHQFQQDILLPFLDE
                     HLRGGPGANLARVTAYNTGEKRWERLDSWPRAGEGDTGLKPLFLHAGYGLSFDRPEQT
                     GGSDSYISDPAKPVPFLPRPVVDPFAAFAAGSLGHAYEAWAQWLVQDQRFVDGRPDVL
                     SYETPVLAEAVRVRGIPVAEIHALTSGTDADFVVKLIDVFPAKDPSAPEHGGYQLPIA
                     MDIFRGRYRDSFEHASAIPANRPQRYRFELPGVNHVFKPGHRIMVQIQSSLFPLYDRN
                     PQTFVPNIFEAKAPDYRKAEIAILRSQSQASAVLLPVVEK"
     gene            5664..7853
                     /locus_tag="VHE37_09100"
     CDS             5664..7853
                     /locus_tag="VHE37_09100"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HVT35730.1"
                     /translation="MVAVVAMASCSRERPLPGREQVSADAGWVTGGHDDAQTYSSPLR
                     QVNAGNVASLGYAWSFDLKTTHGMEATPVVVDGVMYASAPWGLVHALDARSGKLLWTF
                     DPKVDDSITSKVCCGIVNRGLSFARNRVFVASIDGRLFALDAKTGKVLWEVDTIVDHA
                     RGYTVTGSTYVAGDAVLIGNSGAELDARGYVTAYDIATGAQRWRFYTVPASTKGPFEN
                     PELEAAAKTWDPNSFWEVGLGGTVWDGMAYDAELKLVYVGVGNSALYPRKLRSPSGGD
                     NLYVSSILALHADTGRMAWYYQTTPGDQWDFTATQKMILADLQIGGRKRQVLMQAPKN
                     GFFYVLDRVTGELLSANNYIPVTWASHVDLKSGRPIETGQADYSSEPRLVFPGPSGGH
                     NWQPMAFNPQTQLVYIPTQVMPAVYWIPKEPFKYQKGAVNLGALYAFPIDGEMDYGDD
                     ETAHVMPKLAELSKGQPDTTVRGYLQAWDPATGKMVWKVDTSGPWAGTMNAIWNGGGV
                     MTTASGLVFQGRSDGFLYAYKASTGEQLAAIDIGTSIMAAPMTYELDGEQYVAVMAGF
                     GGALGGTHPEGTAAYRYGNAGRIVVVHLNGGAVPHPAELKRETKFARPPAERSASAMT
                     IAQGGELFARHCNRCHKNNAASGTVPDLRRINAQTYADFDQIVLGGIRAEKGMGSFKG
                     ILTPSDVHAIRDAMISEAWRAYDSEQAPSGAATHVPKAETGDAGEKK"
     gene            7877..8338
                     /locus_tag="VHE37_09105"
     CDS             7877..8338
                     /locus_tag="VHE37_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVT35731.1"
                     /translation="MFALVAAAASDYASAQQASALKDGERMVARCLDFATANKYPPLS
                     VAVIDASGSLVLFKRQDGASSASADAALLKARTALRLNAPTSVLGTVAAADAPTRDAF
                     IVMQMTTLPGGSPLQSTDGLAIGAVGVSGGEAEQDAECARMAAEPAPAKKK"
     gene            8591..10978
                     /locus_tag="VHE37_09110"
     CDS             8591..10978
                     /locus_tag="VHE37_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35732.1"
                     /translation="MAPRTATPPDGAPAGSDAEAGATMEPASAEQRAQADATAPLETA
                     AAPATTETVPVIPVAQKDTPDVSATAPHGDTAQLDEIVVTATKRNQSLRKIPSTINVI
                     TGEKLETQGARELQDFVDQIPGLQMQDASVTSSRKIVIRGIAPDDVGNQTVGTLLGDV
                     PMSDAIGSYTVVDPDTWDLETVEVLKGPQGTLFGASSLAGIIRYVPKAPQLGLWQGKA
                     EAEYMSTKDGGAAPAFSAALNVPVGNEFAMRFSGTREKRPGVIDINTPGRQQKDADAA
                     RKWSGRAMLFWQPTERATINAWYMAQQRNADEQFFVTNYNADYTRYDAPSKSPTDRKF
                     AMGVIDARYRFDWATVVSLSALQKKHNNFNIDASYVLLEPAADAGVEVGRTLRKAKAN
                     GFMQELRLVSPDDGPWLWQAGAFYSDFRADVYSDIFLTDPLSIQAALQLIPEAQRSAV
                     ASDNGVSLGSAHLYPLGATEGALFGELTRELGPVNLTLGGRYYKTKTIGTSTVAGIAP
                     FAATGSPVTVEDLQVADKGFSPKGAVTWQATGDILVYGTISRGFQYGGVNNRAVPTPG
                     SNSPRTYKSSALWNYEAGVRTDWLERTLRADFTAFYLDWTNAQISQQIPPADFYIDNV
                     GKVKVKGMEASLRYYFPLDGLSLETTGGYLDSRTAGPFTDGSGNVVAPGAEMPNAPKI
                     QYTATLNYLRTFSDVWSTATALQFNHVGQAFGNIEHTGLLDARNMLNFNFSVSRPDLA
                     FAPALGLIVNNITDQRKIVSSTQSPQGAAADLESAYGVGYTRPRTFILRLSGSFQ"
     gene            10975..13242
                     /locus_tag="VHE37_09115"
     CDS             10975..13242
                     /locus_tag="VHE37_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVT35733.1"
                     /translation="MRSLAMRTWGSLSAAVLAACASLSSHEAGHYEAIITRTSYGIAH
                     IKAADYAGLGYGEAYARAQDNICLLAETYVSYAGERSKYFGAGGDTMIGLGAGKNLDS
                     DFYHRAVLDISGLRDAFESVSEDYKGLVDGWVVGYNRFLQDNAGHLPAPCAGAPWVRA
                     ITRDDVLRSLNAFAVQSTSGMFALPLVNSAPPGVDVPKAARADLTPQDPMLAGSNGWA
                     FGGDATTNGKGVVLANPHFPWMGPNRFYETHLTIPGKLDVAGAAITAMPFVGIGFTRD
                     VAWTHTVDMAAHMTLYRLALDPVDPAAYMVDGNRETMTRRDITVEVKDGDPVTRTLYA
                     SRYGAIVSVPGTKYAWTREAAYALKDANRNNLRMGDGWLDMARARNVQDIREALARHL
                     AAPYFNTMAADREGTALYADINAVPNVPAERFKACGLIEDRLPGQLQDLYVLDGSRSG
                     CEWETSGESPVPGLLPASQQATLYRRDFVQSSNDSYRWSNPSALQRLGAIMGRDPGIG
                     SLRTQSALEAIGAVLKTGKFDPAVAAATMFSNKVFIAPRVLPGLTQLCRRKDANAEAC
                     AALANWDGKANLDSRGAVLFALFWSKVGTRADLWKHPLDADDFANTPRDFVIDGKVGD
                     DLLGALAASVEELKQRKLAPDAALAEAQFTIRGSERIPISGLQRGGTLNYTRTIPNGP
                     VVVVGASYVQAVTYDDNGPVANAVLTYSQSTDPASSHFADQTREFSRLQLHRYPYSDA
                     EIAADAIGAPLTIRQ"
     gene            13257..14135
                     /locus_tag="VHE37_09120"
     CDS             13257..14135
                     /locus_tag="VHE37_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT35734.1"
                     /translation="MLKISNVSHVYPNGVRALDGVSLEVQPGMFGLLGPNGAGKSSLM
                     RCIATLQVPSSGAIQFDSIDVLGNPEALRKTLGYLPQDFGVYPRVSAYHMLDHIAVLK
                     GYAASSERREVVEALLNKVNLWNVRDKAVAGFSGGMRQRWGIAQALMGEPRLVIVDEP
                     TAGLDPEESNRFLNLLAEVAESTVVILSTHIVEDISKLCQRTAMLMGGRIVKEGTPKE
                     LIEAVRGRIWRKTIDKWELDAHRAAHHVIATRLYGGRTFIHVLADTNPGDGFSAVDAS
                     LEDAYFSTLFSGRRAA"
BASE COUNT         2539 a         4662 c         4655 g         2398 t
ORIGIN      
        1 gaccagctgc tcgccgccgg cctcaacttc cgcaactacg gcgagcgcgg cggcggtgcc
       61 aatcccgagc cggcgagcca gggcgccaac cgcacgctgg agcagttcgc gcaggtgcag
      121 ctgcactcga gcccggatta cccgttcaac ggcctgtccg cctgcatgca ggccagcgcc
      181 aatgtcgatc cggcgccgct gttcgagggc tgcatctacg attcgtcggc gcgcacgcag
      241 agccgtctca ccggcgccaa cgtcgcgcct ccgggcgcgg tgtcgcgctt cgcgctgttc
      301 gaggccgagt tcaccccgct ggtgacggcg ggccagtggt acccgtttac ctacctgatc
      361 cagttcaacg accacggcca gggcagcacg ccgaacaaca cgaccgaagc cgcgcaggcg
      421 gcggacaacg atctcgccct gggccagctg gtggacctgg tgtcgcactc gccgatctgg
      481 ccgcagagcg ccatcttcgt catggaagac gacagccagg atggcggcga tcatgtcgat
      541 tcgcaccgca tgccggccta cgtgatctcg ccgtgggcca agcagggcgc cgatgcggcg
      601 cacgcgccgg tcatcgcgca gcgctacgac cagctggcca tgctgcgcac catccagctg
      661 atcctgggcc tgccgtcggc ctcgctgctg cactcgctgg cgtcgcccat gtatgacgtg
      721 ttcatcgatc ccatgcaggc gcccgacctc acgccctaca cggcgatcct gccggagcgt
      781 tcgctggtcg agcaggtcgg caagaccgcg gccagcatgg cctttgcgcg caacgcgccc
      841 gggctctatg cgctgtcgca gaagctgccc tggcatcgca tggacgcggt gccgcaggaa
      901 attgccgacc gcgtgcacta cgccaacctg tggggcgacg accggcacta tcccgggccg
      961 gggccgaatg cctcggccga agagcacgag cgcgccgtgg atttgctgcg cgtgctgaag
     1021 cgcggcggcg atgcgcgcag gctgctgcaa acggcggacg acgactgagc ggcggcggcg
     1081 cgccggctcc gtgcaggcgt cggcgcgccg ccccggttcc cacgcatgaa caccgcagga
     1141 aatacgccac cgaatcacag cctgtgggac ctggtgcgct acgcgctgcg cctgggcacc
     1201 accgggttcg gcgggccggt ggcgctggtc ggctacatgt atcgcgacct ggtcgagcag
     1261 cggaaatgga tcagcgaggc cgactacaag gaaggcctga cgctggcgca gctgatgccc
     1321 ggaccgctgg ccgcgcagct ggccatgtac ctgggctacg tgcattacgg cgtgctcggc
     1381 gcgtcgctgg tcggcgtggc gttcgtggtg ccgtcgttcc tgatggtgct ggcgctgggc
     1441 tggctctacg tgggttacgg cgggctcaac tggatgcagg cggtgttcta cggcgtgggt
     1501 gctgccgtca tcggcatcat cgccatgagc gcgtacaggc tgaccaccag gaacatcggg
     1561 cgcgatccgc tgctatgggg catcttcctg gtcagcgcgg ccatcaccgc gatcaccgcc
     1621 agcgaaatga tcggcgtgtt cctcgccggc ggcatcctgg tgtggctggt gcgcgcgccg
     1681 ccgaagctgg gcggcggcag cgtttccgcg gtcgctgcgc tggcaccggc cgtgggcgcc
     1741 ggcaagggtc cgctgctgat gcagatcttc ctgttcttcg ccaaggccgg cgcggtggtg
     1801 ttcggcagcg gcctggccat cgtgccgttt ctctatggcg gcgtggtgca cgagcatcaa
     1861 tggctcagcg agcagcagtt cgtcgacgcc gtcgccgtcg ccatgatcac gccgggaccg
     1921 gtggtgatca cggtgggctt catcggttat ctcgtcgccg gctttcccgg cgcggcggtg
     1981 gcggcgctgg ccaccttcct gccgtgctac ctgttcacgg tgatccccgc gccgtatttc
     2041 aagaagcacg gcaagcggcc ggcgatcgtc gccttcgtcg atggcgccac ggcggcggcg
     2101 gtgggcacca tcgccggctc ggtggtggtg ctcggccagc gttccatcac cgacctgccg
     2161 acgctggcaa tcgcgcttgc cgcgctgggc gtgatgtggc gctacaagaa agtgcaggag
     2221 ccggtcatcg tgctggccgc ggcggcgctg ggcctgctga tccgcggcct gggatgagaa
     2281 gcgccgcggg tggtgccgcg gcgcatgcac acgcaaaaaa tgcctgaccg ccatgccggg
     2341 atcgatgtta tttattatag tattaattta caaacataca ataataaata acatgccgga
     2401 tggcggtgcg gtggagctct gcatgcgcct gcagcagcac ttcaggcacg cggcggcaag
     2461 tggttgagcc gcacgcgcac gaattcgata tgccggctcg ccgcacgcgt ggcgcccttg
     2521 ccgtcgcgcg cgaggatcat gcgcagcaac tcgcggtgct gggcgcgcac ctggtcggtg
     2581 atgccggact tcggctcggt gccggcctgg gacagctgca tgtgatcgag cagcagccgg
     2641 tgcagtgaac ccatgaggta actgaacacc gggttgtgcg tggcctcggc gatggcgtgg
     2701 tggaaggcga ggtcggtggt cagctggacg cggcggtcgt cggagcgcca cgcttgctcg
     2761 acggccgctg ccgtctgctc cagccgcgcg cggtccttcg cgtcatggcg cgcggcggcc
     2821 agcgcggcgc tgctcgctga tttatgggct agatttgcag gatgagcacg agactgaaca
     2881 tcgatttgtt gcgaaatttc gtggctattg cagaagctcg cgtattgagc cgcgcagccg
     2941 caagagtggg ccgcacgcag gcagcgctaa gccagcaagt caagcgtctc gaagcagaag
     3001 tgcagcagac gctgatgatc cgcggcggcc ggggcattac gttgactgcc cagggcgagc
     3061 ggctgttcct gcacgcgcag aacatcctgc gcactcacga cgaggcggtc ggcgaattgc
     3121 tcggcgtgac cttgtcggga accctgcgca tgggatgccc ggacgactac gcactcgcct
     3181 ttttgccgtc cttgctccgg ggatactcca atctgcatcc gcaggtgatg atcgaggtcg
     3241 cctgcgcgcc gacgccgcgg ctgctggagc gcatcaagaa ccaggaactc gacatcgcca
     3301 tagtgtcgga aacggacagt tcccaacgcg accagcttct gcgcagggag caattcgtct
     3361 gggttggggt caagggcggc gacgtctgca accgcgatcc gctgccgctg gcattgtccg
     3421 atattgacac catggtccac caggcggcga cctcgcggct cgagcgcgcc ggccgcaagt
     3481 atcgaattgc ctatgccagc gacggcatcg ccgggctcac ggccgtggtt cgttcaggcc
     3541 aggccattgc ggtactgacg aagtctgcag tgccgcccga cctttacatc cttcctccat
     3601 cgagcggcct gccgaagctg ccgtcgattg gcatcacggt aaagacggcg cggcgcaatt
     3661 cgtccccgtt gcttcgcagc tttgaagcgc atgtgcgctc agtactgccg acgctgtagc
     3721 gaccattaag gacgcttata gctcacataa gcagtattag ctgtctttcg gcgcggtgcc
     3781 gtcggcagaa tcggcgcatg agcgacattc cggacgactt caaggccagg attctcaacc
     3841 cgggctacgt cgagcgcatc gagatgatcc cgatgcgcga cggcgtgaag ctgaagacct
     3901 acatctacat ccctgccggc gccaagggtg cggcgattct gctgatgcgc acgccgtaca
     3961 acgcctcgac gctgaaagcg aaaatcgcag gcgaccgcta cctggaggcg ggctacatcc
     4021 gtgtcatgca ggacgtgcgc ggcaagtacg gatcggaggg cgagtacgtc gtcacgcgtc
     4081 cggtgcgcgg agcgctcaac gccagcgatg cggatcaccc gacggatacc tgggacacga
     4141 tcgactggct ggtgcagaac ctgcgagagt cgaatgggcg ggtggcgatc ttcggcaact
     4201 cctatgtcgg cttcaccagc gccatggccc tgctcgaccc gcaccctgcg ctcagggcgg
     4261 cggtgccggt ggggccgctg atcgacgcat ggatgggcga cgactggttt cactacggcg
     4321 catttcgcca gatgatgctt ggcttcatcc acaagcagac cgcgcagcgc ggcgaaggcg
     4381 tggtgacgcc cagcgtcacc cacgacaagt acgaggagtt cctgcgagcc ggatccgccg
     4441 ccgactacat ccgcgaacac gggctggacc gcttgccgtt tgcggcgcgc ctgatggcgc
     4501 acccggccta tgacgcgtac tggcaggcgc aggcgctcga caagttgctg gccgcgcgcc
     4561 cgtcgcgcgt gccgaccatg tggacccagg gcctctggga ccaggaggac atgtacggcg
     4621 cgaaccacgc ctggcgagcg ctgaaggcgg ccgggtacga agccaacaac tggctggtgc
     4681 tcgggccgtg gaaccatgtg caggcctggc gcaccgcggc ctcgctggga ccgatgcaat
     4741 ggcatgcgga caccgcgcac cagttccagc aggacatcct gctgccattt ctcgacgagc
     4801 atctgcgtgg cggaccaggg gccaatcttg cgcgcgtcac ggcctacaac accggcgaga
     4861 agcggtggga gcgcctcgat agctggccgc gcgcgggcga aggcgacacc ggcctgaagc
     4921 cgctgttcct gcacgcgggc tatggcctgt cgtttgacag gcctgagcaa accggcggca
     4981 gcgacagcta catcagcgat ccggccaaac ccgttccgtt cctgccgcgc ccggtggtcg
     5041 atccatttgc cgccttcgcc gcagggtcgc tgggccacgc ctacgaagcc tgggcccagt
     5101 ggctggtgca agaccagcgc ttcgtcgacg gccggcccga cgtgctcagt tacgagacac
     5161 cggtgctggc tgaagcagtg cgcgtgcgcg ggatacccgt tgccgagatc cacgcgctta
     5221 cctctggcac cgacgctgat tttgtcgtca agctgatcga tgtgtttccc gcgaaggatc
     5281 caagcgcgcc agagcatggt ggctaccagc tgccaatcgc gatggacatc ttccgcggcc
     5341 gctaccggga cagcttcgag catgccagcg ccataccggc gaaccggccg cagcgctacc
     5401 gcttcgaatt gcccggcgtg aatcacgtat tcaagccggg tcaccgcatc atggtgcaga
     5461 tccagtcgtc attgtttccg ctgtacgacc gcaatccgca gactttcgtg cccaacatct
     5521 tcgaggcgaa ggcgccggac taccgcaagg cggagattgc catcctccgt tcacaatcac
     5581 aggcatcggc cgtgctattg ccggtcgtcg aaaaataaga ggcggtgttt tctatgcagc
     5641 gtcactggtt ttcagccttg ttcatggtgg cggtcgtggc gatggcttca tgtagtcgag
     5701 agcgtccttt gcccggccgg gagcaggtat cggccgacgc aggctgggtc acgggcggac
     5761 atgacgacgc gcagacctac tcgtcgccgc tgcggcaggt caatgccggc aatgtggcca
     5821 gcctgggcta cgcctggagc ttcgacctga agaccacgca cggcatggag gcaacaccgg
     5881 tggtggtgga tggcgtgatg tacgcgagcg cgccatgggg actggtccat gccctcgacg
     5941 cgcgcagcgg gaagctgctg tggaccttcg atcccaaggt cgacgactcg atcaccagca
     6001 aggtctgctg cggcatcgtc aaccgtggcc tgtcgtttgc gcggaaccgg gtgttcgtcg
     6061 cttccatcga cgggcgcctg ttcgcgctcg acgcgaagac gggaaaggta ctttgggaag
     6121 tcgacactat cgtggatcac gcacgcggct acacggtcac tggctccacc tatgtcgcgg
     6181 gcgatgcggt gctgatcggc aattccggtg cggaactcga cgcgcgcggt tatgtgactg
     6241 cttacgacat cgctaccggt gcgcagcgct ggcgcttcta caccgtgccg gcgagcacca
     6301 aggggccgtt cgaaaacccg gaactcgaag ccgccgcgaa gacctgggat ccgaattcct
     6361 tctgggaagt cggtctcggc ggcaccgtgt gggacggcat ggcatacgat gccgaactga
     6421 agctggtcta cgtcggcgtc ggcaactcgg cgctctatcc acgcaagctg cgcagcccga
     6481 gcggcggcga caacctttac gtctcatcga tcctggcact gcacgcagat accggccgca
     6541 tggcctggta ttaccagacc acgccgggcg accagtggga tttcaccgcg acgcagaaga
     6601 tgatcctggc cgacctgcag atcggcgggc gcaagcggca ggtgctgatg caggcgccca
     6661 agaacggctt cttctatgtg ctggatcgcg tcaccggcga attgctgtct gccaataact
     6721 acattcccgt cacctgggcc tcgcacgtcg atctgaagag cggtcggccg atcgagactg
     6781 ggcaggccga ttactccagc gagccgcgcc tggtatttcc tgggccctcc ggcgggcaca
     6841 actggcagcc gatggcgttc aacccgcaga cccaattggt ctatatcccc acgcaggtga
     6901 tgccggcggt gtactggatt ccgaaggagc cgttcaagta ccagaagggc gcggtgaacc
     6961 tgggcgccct gtacgcattt ccaatcgacg gtgaaatgga ctacggcgac gacgagacgg
     7021 cgcacgtcat gccgaagctg gcggaactgt ccaagggcca acccgacacc accgtgcgtg
     7081 gttacctgca ggcctgggat ccggccaccg gcaagatggt gtggaaagta gacacctccg
     7141 gcccgtgggc cggcacgatg aacgcgatct ggaacggtgg cggcgtcatg accaccgcca
     7201 gcggcctggt gttccagggg cgctccgacg gatttcttta cgcgtacaag gccagcaccg
     7261 gcgagcagtt ggcagcgatc gacatcggca ccagcatcat ggccgctccg atgacctacg
     7321 aactggacgg cgagcagtac gtcgcggtga tggcgggctt cggcggcgcg ctgggtggta
     7381 cgcatcccga aggtactgcc gcctaccgct acggcaacgc gggccgcatc gtggtggtgc
     7441 atctgaacgg cggcgccgtg ccgcatcctg cggaactcaa gcgtgaaacg aaattcgcgc
     7501 gcccccccgc cgagcgcagc gcctcggcga tgaccattgc gcagggcggg gaactgttcg
     7561 cgcgccattg caaccgctgc cacaagaaca acgccgccag cggtacggtg ccggacctgc
     7621 gccggatcaa tgcgcagacc tacgccgact tcgaccagat cgtcctcggc ggcatccgcg
     7681 cggaaaaggg catgggatcg ttcaaaggga tcctgacgcc gagtgatgtg cacgccatcc
     7741 gcgacgccat gatcagcgaa gcctggcgag cctatgacag cgagcaggca ccctcaggtg
     7801 cggcaacgca tgttcccaaa gcagaaaccg gcgatgccgg ggagaaaaaa tgatgcgtgc
     7861 cggattgagc aggatcatgt ttgcgcttgt cgccgcggcg gcatcggact acgccagtgc
     7921 acagcaggcc agtgcgctga aggacggcga gcgcatggtt gcacgttgcc tcgacttcgc
     7981 caccgccaat aaatacccgc cgctgagcgt cgcggtaatc gatgcctcgg gctcgctggt
     8041 gttattcaag cgtcaggacg gcgcctcgtc ggccagcgcc gacgccgcgc tgctgaaggc
     8101 acgcaccgcg ctgcgcctga atgcgccgac ctcggtgctg gggacggtgg ccgcggccga
     8161 tgcgccaacc cgcgacgcct tcatcgtcat gcagatgacg accctgccgg gcggatcgcc
     8221 gcttcagtcc accgatggac tcgctatcgg cgccgttggc gtgtcgggtg gagaagccga
     8281 acaggatgcc gagtgcgcgc ggatggctgc agagcctgcg ccggcgaaga agaaatagcc
     8341 acagctgacc ggttaaaccg tgctgtccga ggcccgcgtc gtggcataag aaagttttat
     8401 gaccagcatt acaactatta gttattcgcg gaaatcctct tgcggtaagt tcgccggcat
     8461 gctgaaagaa accctccagg ccgcgactcg cccgctcggc gcggccgcat gtgccgtcgc
     8521 gttgtgcatc ggccctctgt ccctggctgg ggcccaggag ccgccggctg ggacgcagca
     8581 ggatgccgca gtggcgccgc gaacggccac gccgccagat ggcgcgcccg cgggcagcga
     8641 tgcagaagcc ggcgcgacca tggaaccggc ctccgcggaa cagcgcgcgc aagccgacgc
     8701 cactgcgccg ctggagacag ctgcagctcc cgcgacgacc gaaaccgttc ctgtcattcc
     8761 cgtcgcgcag aaagacactc ctgatgtgtc cgcaacagcg ccgcacggcg atacggcaca
     8821 gctcgacgag atcgtggtca ccgcgaccaa gcgcaaccag tcgctgcgca agattcccag
     8881 caccatcaac gtcataactg gggagaagct ggagacgcag ggcgcacgcg agctgcagga
     8941 cttcgtcgac cagattcccg gactgcagat gcaggacgca tcggtcacca gcagccgcaa
     9001 gatcgtgatc cgcggcatcg cgcccgacga tgtcggcaac cagaccgtgg gcaccctgct
     9061 cggcgacgtg ccgatgagcg atgccatcgg cagctacacc gtggtcgacc ccgatacctg
     9121 ggacctggag acggtcgagg tgctcaaggg cccgcaaggc acgctgttcg gtgcctcgtc
     9181 gctggcgggc attatccgct acgtacccaa ggcaccgcag ctgggcctgt ggcagggcaa
     9241 ggccgaggcc gaatacatgt cgaccaagga tggcggcgcg gccccggcct tcagcgccgc
     9301 gcttaatgtg cccgtcggca atgaattcgc catgcgcttc tccgggacgc gcgaaaagcg
     9361 ccccggcgtg atcgacatca atacaccggg tcgccagcag aaggacgcgg acgccgcgcg
     9421 caagtggtcc ggccgcgcga tgctgttctg gcaaccgacc gaacgcgcga cgatcaacgc
     9481 ctggtacatg gcgcaacaac gcaatgccga cgagcagttc ttcgtcacca actacaacgc
     9541 cgactacacg cgttacgacg cgccgagcaa gtcgccgaca gaccgcaagt tcgccatggg
     9601 cgtcattgat gcgcgctacc gcttcgactg ggccaccgtg gtttcgctca gcgcgctgca
     9661 gaagaagcac aacaatttca atattgacgc gtcgtatgtg ctgctcgaac ccgctgccga
     9721 cgccggcgtc gaggtggggc gcacgctgcg caaggccaag gccaatggct tcatgcagga
     9781 actgcggctg gtgtcgcccg acgatgggcc gtggctatgg caggcgggcg cgttctacag
     9841 cgacttcagg gcggacgtgt actccgacat cttcctaacc gatccgctga gcatccaggc
     9901 ggcgctgcaa ttgataccgg aggcacagcg ttcagcggtc gccagcgata acggtgtttc
     9961 cctgggcagc gcgcatctgt accccctcgg cgcaaccgag ggcgcgctat tcggcgaatt
    10021 gacgcgcgag ctgggcccgg tgaacctgac gctcggcggg cgctattaca agacgaaaac
    10081 gatcggcacc tcgacggtcg ccggcatcgc gccgttcgcg gccaccggct cgccggtcac
    10141 cgtggaggat ctgcaggtgg ccgacaaggg cttcagcccc aagggagccg tcacctggca
    10201 ggccaccggt gacatcctgg tctacggcac catctcgcgc ggcttccagt acggcggcgt
    10261 caacaaccgc gcagtcccta ccccgggctc gaactcgccg cgcacgtaca agtcaagcgc
    10321 gctgtggaac tacgaagccg gcgtgcgcac cgactggttg gagcgcacgc tgcgtgccga
    10381 cttcaccgcg ttctaccttg actggaccaa cgcgcagatt tcgcagcaga taccgccggc
    10441 cgacttttac atcgacaacg tcggcaaggt gaaggtcaag ggcatggagg cctccctgcg
    10501 ctactacttc ccgctcgatg gactgtcgct cgaaaccacc ggcggttatc tcgactcgcg
    10561 cacggcgggc ccgttcacgg acggctcggg caacgtggtg gcgccgggtg cagagatgcc
    10621 caatgcgcca aagatccaat acaccgccac gctcaactac ctgcgcacgt tctccgatgt
    10681 ctggtcgacc gcgacggcgc tgcagttcaa ccacgtcggc caggccttcg gaaacatcga
    10741 gcacaccggc ctgctcgatg cgcgcaacat gctcaacttc aacttttcgg tcagccgccc
    10801 cgatctcgcg tttgcacccg cgctcggcct gatcgtcaac aacatcaccg accagagaaa
    10861 gatcgtgtcg tcgacgcaat cgccgcaagg cgcagcggcg gacctcgagt cggcctacgg
    10921 tgtcggatac acgcgcccgc gcaccttcat cctgcgtctc tcaggatcat tccaatgagg
    10981 tcactcgcca tgcgtacctg gggatccctt tccgctgccg tgctcgcggc gtgcgcgagc
    11041 ctgtcctcgc acgaagccgg ccattacgag gcgatcatca cgcgcaccag ctatggcatc
    11101 gcacacatca aggcggcgga ctacgcgggc ctgggctatg gagaggctta cgcacgtgcg
    11161 caggacaaca tctgtctgct cgccgagacc tacgtcagct acgccggcga gcgctcgaaa
    11221 tatttcgggg ccggcggcga cacgatgatc ggactcggcg caggcaagaa cctggacagc
    11281 gatttctacc accgtgcggt gctcgacatt tccgggctgc gcgatgcgtt cgagtccgtg
    11341 tccgaggatt acaagggtct ggtggacggc tgggtggtgg gctacaaccg cttcctccag
    11401 gacaatgcag ggcacctgcc ggcgccgtgc gccggcgcgc cgtgggtccg cgccattacg
    11461 cgcgacgacg tgctgcgctc gctcaacgct tttgctgttc agtccacctc gggcatgttc
    11521 gcgttgccgc tggtgaactc ggcgccgcca ggggtcgatg taccgaaggc agcgcgtgcg
    11581 gacctcacgc cccaggatcc gatgctcgcc ggcagcaatg gctgggcttt cggcggtgat
    11641 gcaaccacga acgggaaggg cgtggtgctg gccaatccgc actttccgtg gatgggaccg
    11701 aaccgattct acgaaacgca cctcaccatt cccggaaagc ttgacgttgc cggcgcggcc
    11761 atcacggcga tgcctttcgt gggcatcggc ttcactcgcg acgtcgcctg gacgcatacc
    11821 gtggacatgg cggcccacat gacgctgtac cgccttgcgc tcgacccggt ggatcccgcc
    11881 gcgtacatgg tggatggcaa tcgagagacg atgactcgcc gcgacattac ggtagaggtc
    11941 aaggacggcg acccggttac gcgcacgcta tatgcgtccc gctacggtgc gatcgtctcg
    12001 gtgcccggga cgaagtacgc gtggacgcgc gaggcggcct acgcgctgaa ggatgccaac
    12061 cgcaacaacc tgcgcatggg cgacggctgg ctcgacatgg cgcgcgcgcg caacgtgcag
    12121 gacatccgcg aggcgctggc gcggcatctg gccgcgccct acttcaacac catggcggcg
    12181 gaccgggagg gtacggcgct gtacgcggac atcaacgcgg tgcccaatgt cccggctgaa
    12241 cgcttcaagg cttgcgggtt gatcgaagac cgccttcccg gccagttaca ggatctctac
    12301 gtgttggacg ggagcagatc gggctgcgaa tgggaaacct ccggcgagtc gccggtgcca
    12361 ggcctgctgc ccgcctcgca gcaggccaca ctgtatcgac gcgatttcgt ccagagcagc
    12421 aacgacagct accggtggag caatccgtcg gcgctgcagc ggctcggggc gatcatgggc
    12481 cgcgatcccg gcatcggcag cctgcgcacg cagtcggcgc tcgaagccat cggcgccgtg
    12541 ctaaagaccg gcaaattcga tcctgccgtc gctgccgcga ccatgttcag caataaggtg
    12601 ttcatcgcgc cgcgcgtgct gccggggctc acgcaactgt gcaggcgcaa ggatgccaat
    12661 gccgaagcct gcgccgcact ggcgaactgg gatggcaagg ccaatctcga ttcccgcggc
    12721 gcggtactgt ttgcgctctt ctggagcaag gtcggcacgc gcgcggacct ctggaagcat
    12781 ccgctcgatg cggacgattt cgccaacacg ccgcgcgatt tcgtgattga cggcaaagtg
    12841 ggcgatgacc tgctcggggc gctggccgcc tcggtcgagg agctcaagca acgcaagctt
    12901 gcgccggacg ccgcgctcgc agaggcacag ttcaccatcc gcggcagtga gcggattcca
    12961 atttccggcc tgcagcgagg cggcacactc aattacacac gcacgatccc caacggtccg
    13021 gtcgtggtgg ttggggcaag ctacgtgcag gcagtgacat atgacgacaa cggcccggtt
    13081 gccaatgccg tcctcactta ttcgcaatcc accgatccag cctcgtcgca cttcgccgac
    13141 cagactcgcg agttctccag gctgcagctg catcgttacc cgtacagcga cgcggaaatc
    13201 gccgcggacg ccatcggcgc cccgctcacg atccgtcaat aaaccggttc aatcccgtgc
    13261 tcaagatcag caacgtttcc cacgtctatc cgaacggcgt gcgcgcactc gacggcgtgt
    13321 cgctcgaagt acagcccggc atgttcggtc tgctgggccc caacggcgcc ggcaaatcgt
    13381 cgctgatgcg ttgcatcgcc acgctgcagg tgccgagctc gggcgcgatc cagttcgatt
    13441 ccatcgacgt gctcggcaat cccgaggcgc tgcgcaagac cctcggttac ctgccgcagg
    13501 actttggcgt atacccgcgc gtgtcggcgt accacatgct cgaccatatc gcggtgctca
    13561 agggctacgc cgccagcagc gagcgccgcg aagtcgtcga ggcgctgctc aacaaggtca
    13621 atctttggaa cgtgcgcgac aaagctgtcg cgggcttctc cggcggcatg cgccagcgtt
    13681 ggggcatcgc gcaggcgctg atgggcgagc cgcgactggt gatcgtggac gagccgaccg
    13741 ccggcctaga cccggaggaa agcaaccgat tcctcaacct gttggcggag gtggccgaga
    13801 gcacggtggt gattctctcc acccacatcg tggaggacat ctccaagctg tgccagcgca
    13861 ccgccatgct gatgggtggg cgcatcgtca aggaaggcac gccgaaggag ctgatcgagg
    13921 ccgtgcgcgg ccgcatctgg cggaagacca tcgacaagtg ggaactggat gcgcatcgcg
    13981 ccgcgcatca cgtgatcgcc acgcgacttt acggcggccg caccttcatc cacgtgctgg
    14041 ccgatacgaa cccgggtgat ggcttcagtg ccgtggatgc cagcctcgag gacgcctact
    14101 tttcgacgct gttcagcggc cgtcgcgccg cctgaagacg atgacgatgc tcgcacctgt
    14161 cgcccgcttc gaactgcgct tccaggcatc aacgccgatg ttctggatca ccagtctcat
    14221 cttcttcagt ctgacgttct ggtgggtggt ctcc
//
LOCUS       DASZZC010000429         7305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_229796,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000429 DASZZC010000000
VERSION     DASZZC010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229796"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1101)
                     /gene="gshB"
                     /locus_tag="VHE37_09125"
     CDS             complement(172..1101)
                     /gene="gshB"
                     /locus_tag="VHE37_09125"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HVT35735.1"
                     /translation="MDPIGSIKPYKDTTLALMLAAQARGWKLGYLEMQDLWLRDGVAM
                     GRAQDLQVRDDNDHWFELGEDRVVPLGGFDAILMRKDPPFDLEYVASTYILERAQEQG
                     ALVVNQPRALRDANEKAFISWFPQCCAPTLISRDAAQLRAFHAEQSEIVLKPLDGMGG
                     RGIFRVGADGLNLGAIIEELTRNGERYVMAQKFIPAISEGDKRILVIDGEPVPYCLAR
                     IPRPGEVRGNLAAGGSGRAQPLTERDRWIAAQVGPKLKQMGLLFVGLDVIGDYLTEIN
                     VTSPTCAREIDAAFGTDIGGQLMAAIGRRLPAA"
     gene            complement(1118..2404)
                     /gene="gshA"
                     /locus_tag="VHE37_09130"
     CDS             complement(1118..2404)
                     /gene="gshA"
                     /locus_tag="VHE37_09130"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HVT35736.1"
                     /translation="MQTPVPHLATARSGPVLELEATLLRRTADIESWFESQWSRNVPP
                     FYSSVDLRNADFKLAPVDTNLFPGGFNNLNPAFEPLCVDALKAAISRVCPTAKGILLV
                     PENHTRNKFYLENVATLHGLIQKAGYNAHIGTLIPEIREPTEIKLEISGRSLWFEPIE
                     RSGKEVFTGDFVPCSVLLNNDLSAGVPPVLEGLDQTIIPPMALGWHARQKTQHFGIYR
                     NVAKEFGELLGLDPWLVDPLFRNCGQINFLKREGEECLVANVEMLLDDIRTKYREYGI
                     TQEPFVIIKSDAGTYGMGVMTARSVDDVRNMNRKARTHMSSAKEGRDVTGAIIQEGVY
                     TNEKIGDATAEPVVYMIDKQVVGGFYRLNSTRSDQENLNAPGMRFEPLAFEECGAAPD
                     MKCGPDERPNRFYAYGVVARLAALAAAREIAATNGG"
     gene            2623..2976
                     /locus_tag="VHE37_09135"
     CDS             2623..2976
                     /locus_tag="VHE37_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT35737.1"
                     /translation="MVIDDSQTIRRTAETLLAREGYTVITAQDGFEALSKIADHQPDI
                     IFIDIMMPRLDGYQACALIKGNPRYGRTPIIMLSSKDGLFDRARGRIVGSDEYLTKPF
                     TKDELIGAVRAHIKK"
     gene            2994..3545
                     /locus_tag="VHE37_09140"
     CDS             2994..3545
                     /locus_tag="VHE37_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVT35738.1"
                     /translation="MNADAPAIATDDPFDLLLQLEGKLRAARLDIAAGQAQSWVGLGF
                     RVGDDWLTAPKDDIREVINPPKLTRVPNARPWLQGVANVRGELLTIVDLRQFLGMPVA
                     EEVRARRVLVFNSERVPAGFLVDEVAGYRQFAASEQRPEMQAGKDPFAPYLLGAFVRE
                     GQPWLAFSLHKVAQSDAFKQAGY"
     gene            3548..>7305
                     /locus_tag="VHE37_09145"
     CDS             3548..>7305
                     /locus_tag="VHE37_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVT35739.1"
                     /translation="MSPRRQQITNGLQWVRNELDQSLARARGLIEQYIENPKDMLPLQ
                     QAFVELHQMRGTAAMIQCFGVSAFGEEMRQAVRELMHDKVKQTDDAYTALLGAIVQIG
                     DYIDALASGMDDCVLVLHPAINELRLARNKSILSEADLFAAQMNSLGLHLPVPAGDQN
                     RQEGFAQAHARKLEPVFMAALLQWLKNQPDNRPALARMGKAAEQIAAVAVTPPVYQLW
                     RTVAAIIEGLLTQTLEDSLELKRLFGTVGKQLKLLADSGEKAAAAKQGDLAFQLLFYA
                     GRSDGQGPRVSSIRQAFELDAYLPPANQVEALRQKIHGPTTQLLSKVAEEIRVDLGQI
                     KDNIDLAVRSAGGGVPDLGETRQRMKRVADTLATLGLQVLQRVVANQVLVIEGANEAN
                     PPSVEAWMDMATAILRVEHSLDSALFRQLRQSPLEQAVGKPELEEHVPHSMDLSEGRQ
                     ALLRESLISLSRLKDSVDTYVRTGESIGLVEAAKTLNEVASGLLILEAYDASALVTRL
                     ERHVKSPNVALLRESSERADRFADAISCIEYFLEGLRDRTPQGEGMLATLGKLLDYLD
                     STTSKEAPERLAPQPAPAAPAPEAAVVAAPAEEIDPEIRSIFVEEAGEVREQLEAAVP
                     NWAHNLQNRDALTTIRRAFHTLKGSGRMVGASSIGDFGWALESMLNRCLESAVAINQD
                     VLETVQQALALLPALIEEFQHGGSGAGEQLKSLVARANRLAAGETSGSAEPDMLAIFH
                     EDAREKLEMIGEWLTAQDRSEQNIAISNDVVRAFHTLRGAAAIVGLTSLAELAGALET
                     FMDSTRGAGLGLTPAGMTLLDGAVGALRQWAEPGTTTPSNAAKPWLDRIDQLQQQVPE
                     SAVQATADRQLAEVFGGEAFDLVSKIEKAAGEWSRAPENRQVPREMLTAFQTLMGAAL
                     MSHAPAIANVSRALHNRMQEAVASSVVPDAGFFTRLGQIIEGIYQQLDSYQLGDFAGD
                     GAALAAEIYGMQWTGEGAESLRELGEIIEMPPVEPSAEFVPPPQAAPVPPPAAYAAEA
                     DPELLSIFLAEAQELLQNLDGYSAAWEQNPQAWQAANEVKRVLHTLKGSARMAALPNI
                     GEVAHRLEKLVVQTTRGEVALDAAFFGRLHQWSDRLHALIDEMQRGSMPDMSALLEEF
                     DTPQPLPKPRVAESAPPAELTGAPSADAEEAPIEMPAVAEDVEAPAVQTVSAQVESAE
                     AFSDIDTDLAEVFTAEAAELLEELEKSLGQLASNPRDTDALRDIQRVL"
BASE COUNT         1322 a         2298 c         2438 g         1247 t
ORIGIN      
        1 cgcgcgcgta catggcggga gcgcgcgcgg tcagcgtggc ctgcgcgccg aggcagggaa
       61 actcgcgctt gacggtcagg cggtcttcgg cggtcggcag gcggctgagc atcagcagca
      121 gcgcgatgat ggcgatggcc gcgacgagtg cggcgcggcg caatccacgt gtcatgcggc
      181 gggcaggcgc cgcccgatcg ccgccatcaa ctggccgccg atgtcggtgc cgaaagcggc
      241 atcgatctcg cgcgcgcagg tgggcgaggt gacgttgatt tcggtgaggt agtcgccgat
      301 cacgtcgagc ccgacgaaca gcagtcccat ctgcttcagc ttcgggccaa cctgcgcggc
      361 gatccagcgg tcacgctcgg tcagcggctg cgcccgtccg ctgccgccgg cggcgagatt
      421 gccacgcacc tcgcccgggc gcggaatgcg ggcgaggcaa tacggtaccg gctcgccgtc
      481 gatcaccagg atgcgcttgt caccttcgct gatcgccggg atgaatttct gcgccatgac
      541 atagcgctcg ccattgcggg tgagctcttc aatgatggcg ccgaggttca ggccatcagc
      601 gcccacgcgg aagatcccgc ggccgcccat gccatccagc ggcttgagca cgatctcgct
      661 ttgctcggcg tgaaacgcac gcagctgcgc ggcgtcgcgc gaaatcagcg tcggggcaca
      721 gcactgcggg aaccaggaga tgaaggcctt ctcgttggcg tcgcgcagcg cgcgcggctg
      781 gttcaccacc agcgcaccct gttcctgtgc gcgttccagg atataggtgg acgcaacgta
      841 ttcgagatcg aacggcgggt ccttgcgcat caggatggcg tcgaaaccgc ccagcgggac
      901 cacgcggtct tcgccgagtt cgaaccagtg atcgttgtcg tcgcgcacct gcaggtcctg
      961 ggcgcggccc atggccacgc catcgcgcag ccacaggtcc tgcatttcga gatagcccag
     1021 cttccagccg cgcgcttgcg cggccagcat cagcgcgagc gtggtgtcct tgtagggctt
     1081 gatggacccg atcggatcca tcaccacgcc cattgtttta gccgccattg gtcgccgcga
     1141 tttcgcgcgc cgccgccagc gctgccaggc gggccaccac gccataggcg tagaagcggt
     1201 tcggacgctc gtccgggccg cacttcatgt cgggcgccgc gccgcattcc tcgaatgcca
     1261 gcggctcgaa gcgcatgccc ggcgcgttga ggttttcctg gtcgctgcgc gtggaattca
     1321 ggcggtagaa gccgccgacc acctgcttgt cgatcatgta caccaccggc tcggccgtgg
     1381 cgtcgccgat cttctcattg gtatagacgc cttcctggat gatggcgccg gtgacgtcgc
     1441 ggccctcctt ggcgctcgac atgtgcgtgc gcgccttgcg gttcatgttg cgcacgtcgt
     1501 caacgctgcg ggcggtcatc acgcccatgc cgtaagtgcc ggcgtcggac ttgatgatga
     1561 caaacggttc ctgcgtgatg ccgtattcgc ggtatttcgt gcgaatgtcg tccagcagca
     1621 tctcgacgtt ggccaccagg cattcctcgc cttcgcgctt gagaaaattg atctggccgc
     1681 agttgcggaa cagcggatcg accagccagg gatcgagtcc cagcagttcg ccgaattcct
     1741 tggcgacgtt gcggtaaatg ccgaaatgct gggtcttctg ccgcgcgtgc cagcccagcg
     1801 ccatcggcgg aatgatggtc tggtccagcc cttcgagcac cggcggcacg ccggcggaaa
     1861 gatcgttgtt gagcagtacg gagcagggca cgaagtcgcc ggtgaagact tcctttccgg
     1921 agcgctcgat cggctcgaac cacagcgaac ggccggaaat ctccagcttg atctcggtgg
     1981 gctccctgat ctcgggaatc aacgtgccga tgtgcgcgtt gtagccagct ttctggatca
     2041 gtccgtgcag cgtggccacg ttttccaggt agaacttgtt gcgcgtgtgg ttttccggca
     2101 ccagcaggat gcccttggcc gtgggacaga cgcgcgagat ggccgccttg agcgcatcga
     2161 cgcaaagagg ctcgaacgct gggttgaggt tgttgaagcc gccggggaac aggttggtat
     2221 ccaccggtgc gagcttgaaa tcggcgttgc gcagatcgac cgacgagtag aacggcggca
     2281 cgttcctgct ccattgcgat tcgaaccagc tctcgatatc ggcagtgcgc cgcagcaggg
     2341 tggcctcgag ttcaaggacg ggaccggacc gcgcggtcgc caggtgggga acaggggttt
     2401 gcatggactt gaaccggtga attgcaccca tggtagcgca gcctttgtag ggattttccg
     2461 caatgtgcgg atcgtatagt cgatcttcaa ggagtgtgtt aaaacacgct cctaaatagc
     2521 aaaggcgcct gacgattaat ccttcagaga tttttcaggc ggcctgcggt tggctccaag
     2581 ggggatgacg tgtccacgtc cgcggaaaac actgccaaag tgatggtgat cgacgacagc
     2641 cagacgatcc ggcgtacagc ggaaacgctg ctcgcgcgcg agggctatac cgtgatcacg
     2701 gcccaggacg ggttcgaggc attgtcgaag attgccgacc accagccgga catcatcttc
     2761 atcgacatca tgatgccgcg gctggacggc taccaggcat gtgcgctgat caagggcaat
     2821 ccgcgctatg gccgcacgcc catcatcatg ctctcttcga aggacggact gtttgaccgc
     2881 gcccgtggcc gtatcgtcgg ctccgacgaa tacctgacca agccgttcac caaggacgag
     2941 ctgatcggcg ccgtccgtgc gcacatcaag aagtaagcag gcaggctgcc ctcgtgaacg
     3001 ccgacgcacc tgccatcgcc actgatgatc ctttcgatct cctgctccag ctcgaaggca
     3061 agctgcgcgc ggcgcgtctc gatatcgctg cgggtcaagc gcagagctgg gtggggctgg
     3121 gcttccgcgt cggcgacgac tggctgacgg cgcccaaaga cgatatccgc gaagtgatca
     3181 atccgcccaa gctcacgcgc gtgcctaatg cgcggccgtg gctgcaaggc gtggccaacg
     3241 tccgtggcga gttgctgacc atcgtcgacc tgcggcagtt cctcggcatg ccggtggcgg
     3301 aagaggtgcg cgcgcggcgc gtgctggtgt tcaattccga gcgcgtgccc gccgggttcc
     3361 tggtggacga ggtcgcaggc tatcgccagt tcgccgccag cgaacagcgg ccggaaatgc
     3421 aggcgggcaa ggatccattt gcgccctacc tgctgggtgc gtttgtgcgc gagggccagc
     3481 cctggctggc attttcgctg cacaaggtgg cgcagtccga tgccttcaag caggcgggct
     3541 actgaccatg tcaccgcgcc gccaacagat caccaacggc ctccagtggg tgcgcaatga
     3601 gctcgatcag agcttggcgc gcgcacgcgg actgatcgag caatacatcg agaatcccaa
     3661 ggacatgctg ccgctgcagc aggccttcgt ggaactgcac cagatgcgcg gcaccgccgc
     3721 catgatccag tgtttcggcg tctcggcgtt cggcgaggaa atgcgccagg ccgtgcgcga
     3781 actgatgcac gacaaggtca agcagaccga cgacgcctac accgcgctgc tgggcgccat
     3841 cgtgcagatc ggcgattaca tcgatgcgct ggcgtcgggc atggatgact gcgtgctggt
     3901 gctgcacccg gccatcaatg aactgcgcct cgcgcgcaac aagagcatcc tgagcgaagc
     3961 cgacctgttt gcggcgcaga tgaattcgct ggggctgcac ctgccggtgc cggcgggcga
     4021 ccagaaccgt caggaaggat tcgcccaggc gcacgcacgc aagctcgagc cggtcttcat
     4081 ggcggcgctg ctgcagtggc tcaagaatca gccggacaac cgcccggcgc tggcgcgtat
     4141 gggcaaagcc gcggagcaga ttgcggcagt ggccgtgacg cctccggtat atcagctgtg
     4201 gcggacggta gcggccatca tcgaaggcct gctgacgcag accctggagg attcgctcga
     4261 actcaagcgc ctgttcggca ccgtcggcaa gcagctcaag ctgctggccg acagcggcga
     4321 gaaagccgcc gcggccaagc agggcgatct tgcattccaa ctgttgttct acgccggccg
     4381 ttccgacggg cagggacctc gcgtcagttc catccggcag gcgtttgaac tcgatgccta
     4441 cctgccaccg gccaatcagg tggaggcgct gcgccagaag attcacggtc cgaccactca
     4501 gctgctgagc aaggtggcgg aggagatccg cgtcgacctc gggcagatca aggacaacat
     4561 cgatctggcc gtgcgctcgg ccggtggcgg agttcctgac ctgggcgaga cccgccagcg
     4621 catgaagcgc gtggccgaca cgctggcgac gctcgggctg caggtgctgc agcgcgttgt
     4681 ggccaaccag gtgctggtca ttgaaggagc caatgaggcc aatcccccct cggtcgaggc
     4741 gtggatggac atggcaaccg caatcctgcg ggtcgagcac agcctggatt cggcattgtt
     4801 ccgccagttg cgtcagtcgc cgctggaaca ggcggtcggc aagccggaac ttgaagagca
     4861 cgtgccgcac agcatggacc tgtcggaagg ccggcaggcg ttgctgcgcg agtcactgat
     4921 cagcctgtcg cggctgaagg actcggtcga tacctacgtg cgcaccggcg aatccatagg
     4981 cctggtggaa gcggccaaga cgctcaacga agtggcatcg ggcctgctga tcctcgaagc
     5041 ctacgatgcc tccgcgctgg ttacgcggct ggagcgccac gtcaagagcc ccaacgtggc
     5101 tttgctgcgc gaatccagcg agcgcgcaga ccgttttgca gacgccatct cgtgcatcga
     5161 atacttcctg gaaggcctgc gcgaccggac accccagggc gaggggatgc ttgccaccct
     5221 gggcaaattg ctggattacc tggattcgac caccagcaag gaagcgccgg aacggctggc
     5281 gccgcagccg gcgccggctg cccccgcgcc cgaagccgca gtggttgccg cgcctgccga
     5341 ggaaatcgac cccgaaatcc gctcgatctt cgtggaggaa gccggcgaag tacgcgagca
     5401 gctggaagcc gccgttccga actgggcgca taacctgcag aaccgcgatg cgctgaccac
     5461 tatccgccgc gcatttcata cgctcaaggg atccggccgc atggttggcg ccagcagcat
     5521 cggcgatttt ggctgggccc tggagagcat gctcaatcgc tgcctggaaa gcgcggtggc
     5581 gatcaatcag gacgtgctgg aaaccgtgca gcaagcgctg gcgttgctgc ccgcgctgat
     5641 cgaggaattc cagcacgggg gcagcggcgc cggtgagcaa ctcaaatccc tggtcgcgcg
     5701 ggccaaccgc ctggcggcgg gagaaaccag cggcagcgcc gagcccgaca tgctcgcgat
     5761 cttccacgag gacgcgcgcg aaaagctgga aatgatcggc gagtggctga cggcgcagga
     5821 tcggtctgaa cagaacatcg ctatttccaa cgatgtggtg cgcgccttcc atacgctgcg
     5881 cggagcggcg gcgattgtcg gtcttacttc gctggcggaa ctggctggcg cgctcgaaac
     5941 cttcatggat tcgacacgcg gcgccggcct cggcttgacg ccggcaggca tgaccttgct
     6001 ggacggtgcg gtcggcgccc tgcggcagtg ggcggagccg gggacgacca cgccctcgaa
     6061 cgcggccaaa ccctggctcg accgcattga ccaactgcag cagcaggtgc ccgaatccgc
     6121 ggtccaggca acggcggatc gtcagctggc ggaagtcttc ggcggcgaag cgttcgacct
     6181 ggtcagcaag atcgagaagg ctgcggggga atggtcacgc gcgccggaga atcgccaggt
     6241 gccgcgcgaa atgctgacgg cattccagac gctgatgggc gccgctctca tgtcgcatgc
     6301 gccggcgatc gccaatgtct cgcgcgcctt gcacaatcgc atgcaggaag cggtcgcttc
     6361 gtcggtggtg ccggatgccg ggttctttac gcgcctcgga cagatcatcg agggaattta
     6421 ccagcaactc gacagctacc aactcggcga ttttgccggc gatggcgcgg ccctggcagc
     6481 cgagatttac gggatgcagt ggaccggcga aggcgcggag agcctgcgcg agcttggcga
     6541 aatcatcgag atgccgccgg tcgaaccgtc cgccgaattc gtgccgcctc cccaggctgc
     6601 gccggtaccg ccgccggctg cgtatgctgc cgaagccgat cctgaactgc tgtccatctt
     6661 cctggccgag gcgcaggagt tgctgcagaa cctcgatggc tactccgctg cgtgggaaca
     6721 gaatccgcag gcctggcagg cagccaacga agtcaagcgc gtgctgcaca cgctgaaggg
     6781 ttcggcgcgc atggccgcct tgccgaatat cggcgaggtg gcgcaccggc tggaaaagct
     6841 ggtggtgcag accacccgcg gcgaggtggc gctggatgca gcattcttcg gacgcctgca
     6901 tcaatggtcc gaccgtctgc acgcgctgat cgacgagatg cagcgcggca gcatgccgga
     6961 catgagcgcg ctgttggagg agttcgatac tccgcagcct ttgccaaaac ccagggtcgc
     7021 ggaatccgcg ccgccggccg agctcactgg agcgccgtcc gcagacgccg aagaagcgcc
     7081 gatcgaaatg cccgcggtgg cagaggatgt agaggctccc gctgtgcaga cggtgtcggc
     7141 gcaggtcgag agtgcggaag cgttcagcga catcgatacg gacctcgcgg aggtgttcac
     7201 cgctgaagcc gccgaactgc tcgaggaact ggagaagtcc ctgggccagc tggcgtccaa
     7261 tccacgcgac accgacgccc tgcgcgacat ccagcgcgtg ctgca
//
LOCUS       DASZZC010000430         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_804075,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000430 DASZZC010000000
VERSION     DASZZC010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804075"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VHE37_09150"
     CDS             complement(<1..970)
                     /locus_tag="VHE37_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HVT35740.1"
                     /translation="MRALASSALYWLVWLLFRPFDWVLKYRTAPINLRDSLKLDPTKP
                     VVWVLSTRSWSDMFVLDRLCRDLHLPRPSRTGLNFPSVDKAGVVYLPALLETRMHATE
                     LTRLIETAIATHDYDAQVVTVSVFWGRDPGQETSLFKLLFADSVQAGAVQKLFIMLFN
                     GRAVFMNFGLPFSFREFADKSGDAPSTMRKLTRVLHFHFLRARTAALGPTLLRRNVVV
                     TGVLQHKSVRRAVEHVASVKGQSYEQTLRYARKCAEEIAADYTTAAINFLERFLGTIV
                     WKRVFADIDLRGIEPVRELAQSHEIIYMPSHRSHADYLLMSYSLYHQ"
     gene            1107..>2889
                     /locus_tag="VHE37_09155"
     CDS             1107..>2889
                     /locus_tag="VHE37_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HVT35741.1"
                     /translation="MRRIAPALVIAWFLVPCTTVAATPPAPAAPATTAADADQFIAGI
                     NHDLRGLLNYYNVAAWVQETYITDDTQKLGARANEEFLAYTAQKLQESKRYNGLSGLS
                     PDTARSLMLLKNVSAPPPSDAAKQAELAKILSDMDGHYGSAKWCRSSTDCLNLTEIEK
                     ILDDPRQTPEARATAWAGWHEAARPIKAEYARFVQLANEGAREMGFADTGEVWRGGYD
                     MTPAEFQAEVERLWAQVQPLYEQLHCYVRAKLNAKYGDRLVPKQGLIPAHLLGNMWAQ
                     QWSNIYPLVEPYPGAGQLDVSAALKKQRDDEYHRLLAAYKGAKTPQALDDLAHQADSA
                     QSVRMAKMAEDFYVSLGFPHLPASFWKNSMLTRPRDREVVCHASAWDLDMSGDVRIKM
                     CIEPNEDDLITIHHELGHIYYDLSYNALPPLFQNAAHDGFHEAIGDTMTLSLTPAHLH
                     KIGLAPAVKPDARALINSQMKWALDKIVFLPWGKLVDQWRWQVFSGEVKPEQYNDAWW
                     KLRARYQGVSPPVTRSADDFDPGAKYHVASNTPYTRYFLSFVLQFQFQKALCDAAGFK
                     GPLNQCDIYGNAEAGRRYAAMLAAGASR"
BASE COUNT          529 a          962 c          963 g          435 t
ORIGIN      
        1 cctggtgata gagcgagtag ctcatcagca ggtaatcggc gtgcgagcgg tgcgacggca
       61 tgtagatgat ttcgtgcgac tgcgccagtt cgcggaccgg ctcgatgccg cgcaggtcga
      121 tgtcggcgaa cacgcgcttc cacacgatgg tgccgagaaa acgttcgagg aaattgatcg
      181 ccgcggtggt gtagtcggcg gcgatctcct cggcgcactt gcgcgcgtaa cgcagggtct
      241 gctcgtagct ctggcccttg acgctggcca cgtgctccac cgcgcggcgc acgctcttgt
      301 gctgcagtac gccggtcacc accacatttc ggcgcagcag cgtgggcccg agcgcggcgg
      361 tgcgcgcgcg caggaagtgg aagtgcagca cgcgcgtgag cttgcgcatg gtgctcggcg
      421 catcgcccga cttgtcggcg aactcgcgga acgagaacgg caggccgaag ttcatgaaca
      481 ccgcgcggcc attgaacagc atgatgaaca gcttctgcac cgcaccggcc tgcacgctgt
      541 cggcgaacag cagcttgaac aggctggtct cctggccggg atcgcggccc cagaacaccg
      601 acacggtcac cacctgcgcg tcgtagtcat gcgtggcgat ggcggtctcg atcagccgcg
      661 tgagctcggt ggcatgcatg cgcgtctcga gcagcgccgg caggtagacc acccccgcct
      721 tgtccacgct ggggaagttc aggccggtgc gcgagggccg cggcaggtgc aggtcgcggc
      781 acaggcggtc gagcacgaac atgtcggacc acgagcgcgt cgacagcacc cagaccaccg
      841 gcttggtcgg atcgagcttg aggctgtcgc gcaggttgat cggcgcggtg cggtatttca
      901 gcacccagtc gaatggccgg aacagcagcc agaccagcca gtacagtgcg ctcgaagcca
      961 gtgcccgcat ggttttcctg cttgttctcg cctgtccgcg tgggccgcaa gtttactatc
     1021 agtcgccctg cgctccctgg tgggaatccc cttccagcgc cgccggacgc cccgttatgc
     1081 tccgcggaaa ccgacggagc acgcacatgc gccgcatcgc acccgcactg gtcatcgcct
     1141 ggttcctcgt gccctgcacc acggtcgcgg caacgccgcc ggcgccggcc gcaccggcaa
     1201 caaccgcggc cgacgccgac cagttcatcg ccggcatcaa ccatgacctg cgcggattgc
     1261 tgaattacta caacgtggcg gcgtgggtgc aggagaccta catcaccgac gacacgcaga
     1321 agctcggcgc gcgcgccaac gaggagttcc tcgcctacac cgcgcagaag ctgcaggagt
     1381 ccaagcgcta caacggcctg agcggactgt cgcccgacac cgcgcgcagc ctgatgctgc
     1441 tgaagaacgt gtcggcgcca ccgccgtcgg atgcggccaa gcaggccgag ctggcgaaga
     1501 tcctgtccga catggacggc cattacggca gcgccaagtg gtgccgcagc agcacggact
     1561 gcctgaacct gaccgagatc gagaagatcc tcgacgatcc caggcagacc ccggaggcgc
     1621 gcgccaccgc ctgggccgga tggcacgagg ccgcccgccc gatcaaggcc gagtatgcgc
     1681 gcttcgtgca gctggccaac gaaggcgcga gggaaatggg ctttgccgac accggcgagg
     1741 tctggcgcgg cggctacgac atgacgccgg cggaattcca ggccgaggtc gagcggctgt
     1801 gggcgcaggt gcagccgctg tacgagcagc tgcattgcta cgtgcgcgcc aagctcaatg
     1861 cgaagtacgg cgaccggctg gtgccgaagc agggactgat cccggcgcac ctgctgggca
     1921 acatgtgggc ccagcagtgg agcaacatct acccgctggt cgaaccctac ccgggcgcgg
     1981 gccagctcga tgtgagcgcc gcgctgaaga aacagcgcga tgacgagtac caccggctgc
     2041 tggccgccta caagggcgcc aaaacgccgc aggcgctgga cgatcttgcg caccaggccg
     2101 acagcgcgca gtcggtgcgc atggccaaga tggcggagga tttctacgtc tcgctcggct
     2161 tcccgcacct gcccgcgtcg ttctggaaga actcgatgct cacgcggccg cgcgaccgcg
     2221 aggtggtgtg ccacgcctcc gcctgggatc tcgacatgag cggcgacgtg cgcatcaaga
     2281 tgtgcatcga gccgaacgag gatgacctga tcaccatcca ccacgaactc ggccacatct
     2341 attacgacct gtcgtataac gcgctgccgc cgctgttcca gaacgcggcg cacgacggct
     2401 tccacgaagc catcggcgac accatgacgc tgtcgctcac gcccgcgcac ctgcacaaga
     2461 tcgggctggc cccggcggtg aagccggatg ccagggcgct gatcaacagc cagatgaaat
     2521 gggcgctgga caagatcgtg ttcctgccgt ggggcaagct ggtcgaccag tggcgctggc
     2581 aggtgttttc cggcgaggtg aagccggaac agtacaacga cgcctggtgg aagctgcgcg
     2641 cgcggtacca gggcgtgagc ccgccggtga cgcgcagcgc ggatgacttc gatccgggcg
     2701 ccaagtacca cgtggccagc aacacgccgt acacgcgcta cttcctgtcg ttcgtgctgc
     2761 agttccagtt ccagaaggcg ctgtgcgatg ccgccggctt caagggcccg ctcaaccagt
     2821 gcgacatcta cggcaatgcc gaagcgggca ggcgctacgc cgcgatgctg gccgccggcg
     2881 cttcgaggc
//
LOCUS       DASZZC010000431         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_613580,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000431 DASZZC010000000
VERSION     DASZZC010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613580"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2093)
                     /locus_tag="VHE37_09160"
     CDS             complement(<1..>2093)
                     /locus_tag="VHE37_09160"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35742.1"
                     /translation="DGTLRNLTAEAGFGLPSGPQDGPNAIAVRQPTVHWSGTKAVFAM
                     LVGGAPKQYAVSTSYWQIYEVTGLAQGDTAVITKLPCQPANYNNIAPLYTSDDQIVYV
                     SDRPRDGQAFLYPQRDEYESQPIETGLWKLDLTHCSYKLLEHVVSGVTYPSIDSFGRL
                     VFTRWDHLQRDQQADADQFENSSFKNGAFNYASETSSTVPTTTSLTEYFPELRDPKFS
                     TPGGTSIAANYPYNAHTFNQFFPWMLRQDGSGEETINHVGRHELGGGYSQGNFRSDGN
                     LHDTSIPGKYNGATYFLTGDHGTFHLREDPKHRGRYYGVNAPEFSTGTGGDLLYITGG
                     PSVNPQSMKVTLVGKYSDYGQFRNPLPMADGTLVVVHTATTDVEKNTGTATAPQYSYA
                     WRMRVVHPHTGGTLTLGSMVTGGINKSVSYWDPDQRVSWTGNLWELDPVEVRARTVPN
                     NTSEPKMPDPEQQVLSAQGVDENLLKYWLKQNSLAMIVSRNVTTRDRADQQQPYNLQI
                     AGSTTQTRGTTTGALYSISHLQLFQADQLRGYTNNGKTLENGGTVRPGRRPIAVPLHD
                     SAAVSAMGAAYNAALNAVPLGSDGSMAAFVPAQRAMTWMLIDSSKSGWAQGIVRERNW
                     LSFKPGERRVCSSCHGVNTVDQAGNAPPANPPEALGTLLTAWKDVVRNNCPNTGGTGH
                     WAYPATFSSCDEGKQ"
BASE COUNT          270 a          648 c          761 g          414 t
ORIGIN      
        1 aactgcttgc cctcgtcgca gctgctgaac gtggccggat aggcccagtg gccggtgccg
       61 ccggtgttcg ggcagttgtt gcgcaccacg tccttccatg ccgtgagcag cgtgccgagc
      121 gcttccggcg gattggccgg cggcgcgttg ccggcctggt cgaccgtgtt gacgccgtgg
      181 cacgagctgc aaacgcggcg ctcgcctggt ttgaacgaca gccagttgcg ctcgcgcaca
      241 atgccctgcg cccagccgct cttgctgctg tcgatcagca tccaggtcat ggcgcgctgc
      301 gcggggacga acgcggccat ggagccgtcg ctgcccagcg gcaccgcgtt gagcgcggcg
      361 ttgtaggcgg cgcccatggc gctcaccgcg gcgctgtcgt gcagcggcac cgcgatgggc
      421 cggcggcccg ggcgcaccgt accgccgttc tccagcgtct tgccgttgtt ggtgtagccg
      481 cgcagctggt cggcctggaa cagctgcagg tgcgagatgc tgtacagcgc gccggtggtg
      541 gtgccgcgcg tctgcgtggt cgagccggcg atctgcaggt tgtacggttg ctgctggtcg
      601 gcgcggtcgc gcgtggtgac gttgcgcgac acgatcatcg ccagcgaatt ctgcttcagc
      661 cagtacttga gcaggttctc gtcgacgccc tgcgccgaca gcacctgctg ctcggggtcg
      721 ggcatcttcg gctcgctggt gttgttgggc acggtgcgcg cgcgcacttc caccgggtcg
      781 agttcccaca ggttgcccgt ccagctgacg cgctggtccg ggtcccagta gctcacgctc
      841 ttgttgatgc cgccggtgac catgctgccc agcgtgagcg tgccgccggt gtgcgggtgc
      901 accacgcgca tgcgccaggc atagctgtac tgcggcgcgg tggcggtgcc ggtgttcttt
      961 tccacgtcgg tggtggcggt gtgcaccacc accagcgtgc cgtcggccat cggcagcggg
     1021 ttgcggaact gtccgtagtc gctgtacttg ccgaccagcg tgaccttcat cgactgcggg
     1081 ttcacgctcg ggccgccggt gatgtacagc aggtcgccac cggtgccggt ggagaactcc
     1141 ggtgcattca cgccgtagta gcgcccgcgg tgcttcggat cttcgcgcag gtggaaggtg
     1201 ccgtgatcgc cggtgaggaa ataggtggcg ccgttgtatt tccccggaat cgaggtgtcg
     1261 tgcaggttgc cgtcgctgcg gaagttgccc tggctgtagc cgccgcccag ctcatgccgc
     1321 ccgacgtggt tgatggtctc ttcgcccgag ccgtcctggc gcagcatcca cgggaagaac
     1381 tggttgaagg tgtgcgcgtt gtacgggtaa ttcgccgcga tgctggtgcc gcccggcgtg
     1441 gagaatttcg gatcgcgcag ctcggggaaa tactcggtga gcgaggtcgt ggtcggcacc
     1501 gtgctcgagg tttcgctggc gtagttgaat gcgccgttct tgaagctgct gttctcgaac
     1561 tggtcggcgt cggcctgctg gtcgcgctgc aggtgatccc agcgggtgaa gaccaggcgg
     1621 ccgaagctgt cgatcgacgg ataggtgaca cccgacacca cgtgctccag cagcttgtag
     1681 ctgcagtggg tgaggtcgag tttccacagg ccggtttcga tcggctgcga ttcgtactcg
     1741 tcgcgctgcg ggtagaggaa cgcctgtccg tcgcgcggcc ggtcggagac gtaaacgatc
     1801 tggtcgtccg aggtgtagag cggcgcgatg ttgttgtagt tggccggctg gcacggcagc
     1861 ttggtgatga ccgcggtgtc gccctgcgcc agcccggtca cttcgtagat ctgccagtac
     1921 gaggtgctca cggcgtactg cttgggtgcg ccgccgacca gcatggcgaa caccgccttg
     1981 gtgccgctcc agtgcacggt gggctggcgc acggcgatgg cgttggggcc gtcctgcgga
     2041 cccgagggca gaccgaagcc ggcctcagcg gtgaggttgc gcagcgtgcc gtc
//
LOCUS       DASZZC010000432         4717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_357457,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000432 DASZZC010000000
VERSION     DASZZC010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357457"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VHE37_09165"
     CDS             <1..210
                     /locus_tag="VHE37_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35743.1"
                     /translation="VVLVAASLEVHPGTVSGVIHGNSTSRRIATKIARTVGMPLSRLW
                     PGRYESRSRPSRARRLSESRAAVSG"
     gene            203..730
                     /locus_tag="VHE37_09170"
     CDS             203..730
                     /locus_tag="VHE37_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35744.1"
                     /translation="MANRGQFFSSAQGDFFQRLEEAPPTAADLDIGLELLGAINTAIR
                     EAKGRGMSRDRIVERMNDLLPDAPKQITKRQLDAWTAQSKEHHEFPARYLPAFCAATD
                     CELPLRVLAQAIGRELVHARDLAAKRLGENIIERARLKREERDLARQLGHVSPGSPPP
                     GGPSTPRTRRSSGAF"
     gene            813..1808
                     /locus_tag="VHE37_09175"
     CDS             813..1808
                     /locus_tag="VHE37_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35745.1"
                     /translation="MQALAQLPKPLQQYLQQTLVQIGDLGGDTHGLWAAAERGLQMEA
                     AGQILAGRALIELREHLPQGAFSSGLADRNIARRSAYNAIEAFEVFAELPNLDSVRAL
                     AQLGITKARALKHWSPDEHKAFASGKPVRGIQLDAAVELSSRDLVEQQRDWQLQNESQ
                     LRKLQSSNATLETKVETLQNEITRLQRAGSYVVAEEDLPDFARTVRQEALANTEQMAW
                     CLDALQAAAEEHLFAKGKSHPSEFKFKPIAAGTLYYSLAAIHARAQLLLKRIQDQFGD
                     SATGMLTIDMQLSPAEIVRFQEQREQLLATHQAKAKARDNQRENTRPGKRGAKRK"
     gene            1813..3675
                     /locus_tag="VHE37_09180"
     CDS             1813..3675
                     /locus_tag="VHE37_09180"
                     /inference="COORDINATES: protein motif:HMM:NF020859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HVT35746.1"
                     /translation="MAALLKFETQPLPLPTAPMPVDPLRLLPDYARVEAERCLAIIRP
                     ALIRIQQGVSRRAASQWLASTAETMPSAPTIDRWLRDYLTGGLIELAPKYQPRQRKDQ
                     PWMARAVELYGKSPTKPAYATVAKWLRDENILPQPQGPRAVRDMRAQNKAVERYLKSV
                     PSSKAETSPLRLGRHFYNQNVKPHVIRDNTVLPVGFIYEGDGHTCDVYVAHPVTGMPF
                     RPELTTWLDVRSHYVTSWWMSEAESAQTTLFSLSQALVGHNHVPAYVHTDPGSGFKAR
                     LISSEVTGFLVRFSIEPILALPGNAKGKGLQEGWYRWFEERCGKRFATFCGHDRSDDF
                     LRHLTAKVKRGEIVLPTLQQYMDAVREYIAQYNATVQEALGCAPADLWAQLQRVELHT
                     PAEAVIRPRIKRTVVRSRVRLDGRKYEAHELGRDHEGRDVIVEYSIHDDGRVAIHDLS
                     GRFVCEARLVGKKAWLPSSRIEEGQQRRLEGQRQRHQRALDEQAARARSPLPIAQPQA
                     GTLQAGHSLVMQAPAPLPMPEPQIDDRTAAILATLPAPEAASARPARRPLSTEEEIAA
                     RFERAQRIGQQLAAGADVPADDIEWFNRYRTTAEYRGQMRFLSSFGDSAGNASA"
     gene            3702..4454
                     /locus_tag="VHE37_09185"
     CDS             3702..4454
                     /locus_tag="VHE37_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT35747.1"
                     /translation="MAKGDGIKGDSVFAGITNVALAMRAMERAVSRPQHLPGMVGFFG
                     DSGLGKSFAAAYTANHFRAYYIECREAWSKKAFLLKLVEDMGISKPGRTVAELTDQAA
                     MQLADSQRPLIVDEADKPVDHGYIEIIRDLHEAAGGAPILLIGEEALEVKLRPFERFH
                     NRMLEWVPAQPASMDDAKKLATLYCPGIKVADDLLTRVLDVSRGVTRRICVNLSRVAE
                     FAALEAPKDNTVTLAMWGTRELSTGEARRRHA"
     gene            4451..>4717
                     /locus_tag="VHE37_09190"
     CDS             4451..>4717
                     /locus_tag="VHE37_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35748.1"
                     /translation="MTQAPATVRVVTSLEERAALRARRGRKPVDLVVDPRHPAGRDAV
                     WHAIRALRKFTVGDLERATRQPQDTLRSYLQGLQKAGFITRNERA"
BASE COUNT          839 a         1679 c         1532 g          667 t
ORIGIN      
        1 gttgtcctgg tcgccgcgag ccttgaagtt cacccgggca cggtcagcgg cgtaatccac
       61 ggcaacagca ccagtcgccg catcgccacc aagattgccc gcaccgtcgg catgcccctc
      121 tcccgcctct ggccgggacg ctacgaatcg cgctcccgcc ctagtcgtgc aaggagatta
      181 agcgaatcga gggccgctgt cagtggctaa tcgcggccaa tttttctcca gcgcccaggg
      241 cgactttttc cagcgattgg aagaagcccc tcccacagcc gcggatctgg acatcggcct
      301 ggagctcctg ggtgctatca acaccgccat ccgcgaggcc aagggccgcg gcatgagccg
      361 tgatcgcatc gtcgagcgca tgaacgacct gctgcccgac gcgccaaagc agatcaccaa
      421 gcggcagctc gacgcctgga ccgctcaatc caaagagcac cacgagtttc ctgctcgcta
      481 cctgccggcc ttctgtgcag ccaccgactg cgagctgccg ctgcgcgtcc tggcgcaggc
      541 catcggccgc gagcttgtcc acgcgcgcga cctggccgcc aagcgcctgg gcgaaaacat
      601 catcgagcgt gcccggctga agcgcgagga gcgggatctc gcgcgccagc tgggccatgt
      661 ctcccccggc tcgcctcccc caggcgggcc atccactccc cggacgagac gatcgtccgg
      721 ggccttttaa ccagacctaa aactcaggga gagcaagggg aatgggaagc aaagatcagg
      781 aaaaagcggt tgtgccagca gctggcacaa ctgtgcaagc gctggcacaa ctgcccaaac
      841 cgctgcagca gtacttgcag caaacgctgg tgcagatcgg tgatctgggc ggcgacacgc
      901 acgggctctg ggcggccgcg gagcgagggc tgcaaatgga agcggccgga cagatcctcg
      961 ccggacgcgc tttgatcgag ctgcgcgaac accttccgca aggcgcgttt tcttcgggcc
     1021 tggccgatcg caacattgcg cgccgcagcg cctacaacgc gatcgaagcc ttcgaggtgt
     1081 tcgcggaact gcccaacctc gacagcgtgc gcgcgctcgc gcagctaggc atcaccaagg
     1141 ctcgcgcgct gaagcattgg tcgcccgatg agcacaaggc cttcgccagc ggcaagccgg
     1201 tgcgcggcat tcagcttgat gctgccgtcg agctttcctc gcgtgacctg gtcgagcagc
     1261 agcgcgactg gcagctgcag aacgaaagcc agctgcgcaa gttgcagtcc agcaatgcga
     1321 ctttggagac caaggtcgag acgctgcaga acgaaattac gcgtctgcag cgcgcgggtt
     1381 cctacgtggt tgccgaggag gatctgcctg acttcgcgcg caccgtgcgc caggaggcgc
     1441 tggccaacac cgagcagatg gcttggtgcc tggatgcgct gcaggctgcc gccgaggagc
     1501 acctgttcgc caagggtaag tcgcatccca gcgagttcaa attcaagccc atcgccgcgg
     1561 gcacgctgta ctactcgctg gccgccatcc atgcgcgcgc gcagctgctg ctcaagcgca
     1621 tccaggacca gttcggcgac tccgccaccg gcatgctcac catcgacatg cagctgtcgc
     1681 cggcggagat cgtccgcttc caggagcagc gcgagcagct cctcgccacg caccaggcca
     1741 aggccaaggc gcgcgacaac cagcgcgaga acactcgccc cggcaaacgc ggagcgaaga
     1801 ggaagtagcg cgatggccgc cttgctcaag ttcgagaccc agccgctgcc gctgcccacc
     1861 gcgccgatgc cggtggaccc gctgcgcctc ttgccggatt acgcgcgcgt cgaggccgag
     1921 cgctgcctcg ccatcatccg tccggcgctc atccgcattc agcagggcgt gagccggcgc
     1981 gccgcctcgc agtggctggc cagcaccgcg gagaccatgc cgtcggcacc gaccatcgac
     2041 cgctggctgc gtgactacct cactggtggc ttgattgaac tggcgccgaa gtaccagccg
     2101 cgccagcgca aggatcagcc gtggatggcg cgagcggtcg agctctacgg caagtcgccg
     2161 accaagccgg cctatgccac cgtggccaag tggctgcgcg acgagaacat cctgccgcag
     2221 ccccaggggc cgcgcgccgt gcgcgacatg cgtgcgcaga acaaggcggt ggagcgctac
     2281 ctcaagagcg ttccgtcgag caaggcggag accagcccgc tgcggctcgg gcgccacttc
     2341 tacaaccaga acgtgaagcc gcacgtcatc cgcgacaaca ccgtgctgcc ggtgggcttc
     2401 atctacgagg gcgacggcca cacctgcgac gtgtatgtcg cgcaccccgt cactggcatg
     2461 ccattccgcc cggagctcac cacctggctc gacgtgcgca gccactacgt caccagctgg
     2521 tggatgagcg aggccgagag cgcgcagacc acgctgttct cgctcagtca ggcgctggtg
     2581 ggccacaacc atgttccggc ctacgtgcac accgatccgg gctccggttt caaggcgcgc
     2641 ctgatcagca gcgaagtcac cggcttcctg gtgcggttct ccatcgagcc catcctggcg
     2701 ctgcccggca acgccaaggg caagggcctg caggaaggct ggtaccggtg gttcgaggag
     2761 cgctgcggca agcgtttcgc aacgttctgc gggcacgacc gcagcgacga cttcctgcgc
     2821 cacctcaccg ccaaggtgaa gcgcggcgag atcgtgctgc cgacgctgca gcagtacatg
     2881 gacgcggtgc gcgagtacat cgcgcaatac aacgccaccg tgcaggaggc gctcggctgc
     2941 gcgccggcgg atctgtgggc ccagctgcag cgagtcgagc tgcacacgcc ggccgaggcg
     3001 gtcatccgcc cgcgcatcaa gcgcaccgtg gtgcgctcgc gcgtgcggct cgacggccgc
     3061 aagtacgaag cgcacgagct cggccgcgac cacgagggcc gcgacgtcat cgtcgaatac
     3121 agcattcacg acgacggccg tgtcgccatc cacgacctca gcggccgttt cgtctgcgag
     3181 gcgcgcctgg tcggcaagaa ggcctggctg ccgtcgagcc gcatcgagga aggccagcag
     3241 cgtcgcctgg aaggccaacg ccagcgccac cagcgtgcgc tcgacgagca ggcagcccgc
     3301 gcgcgctcgc cgctgccgat cgcgcagccg caggccggca cgctgcaggc cggccactca
     3361 ctggtgatgc aggcgcccgc gccgctgccc atgcccgagc cgcagatcga cgatcgcacc
     3421 gccgccatcc tcgccacgct gccggcaccc gaggccgcgt ccgcgcgccc ggcgcgccgg
     3481 ccgctgtcga ccgaggagga gatcgccgcg cgcttcgagc gcgcgcagcg catcggccag
     3541 cagctcgctg ctggcgccga cgtgccggcc gacgacatcg agtggttcaa ccgttaccgc
     3601 accaccgcgg agtaccgcgg ccagatgcgc tttctttcca gcttcggcga ttccgccggc
     3661 aacgcgtccg cctgacggac gctcacccag gagattttgc gatggcaaaa ggcgatggca
     3721 tcaagggcga cagcgtgttc gccggcatca ccaacgtggc gttggccatg cgcgcgatgg
     3781 agcgcgcagt ctcgcgcccg cagcacctgc cgggcatggt cggcttcttc ggcgacagcg
     3841 gcctcggcaa gagcttcgcc gcggcctata ccgccaacca tttccgcgcg tactacatcg
     3901 agtgccgcga ggcctggagc aagaaggcct tcctgctcaa gctggtcgag gacatgggca
     3961 tcagcaagcc cggccgcacc gtcgccgagc tcaccgacca ggccgcgatg cagctggccg
     4021 acagccagcg cccgctgatc gtcgacgagg ccgacaagcc cgtcgaccac ggctacatcg
     4081 agatcatccg cgacctgcac gaggccgcgg gcggcgcacc catcctgctc atcggcgagg
     4141 aagcgctgga agtgaagctg cggccgttcg agcgcttcca caaccgcatg ctggaatggg
     4201 tgccggcgca gccggcgtcg atggacgacg ccaagaagct cgccacgctc tactgccccg
     4261 gcatcaaggt ggcggacgat ctgctcacgc gcgtgctcga cgtcagccgc ggcgttaccc
     4321 gccgcatctg cgtcaacctc tcgcgcgtcg ccgagttcgc cgcgcttgaa gcgccaaagg
     4381 acaacaccgt cacgctggcc atgtggggca cgcgcgagct gtccaccggc gaggcgcgcc
     4441 ggaggcacgc gtgacccagg cgccggccac cgtcagggtc gtcacctcgc tggaagagcg
     4501 ggcggcgctg cgcgctcggc gcgggcgcaa gccggtcgac ctggtcgtcg accccaggca
     4561 tcccgccggg cgcgatgccg tatggcatgc aattcgcgca ttgcggaaat tcaccgtggg
     4621 cgatctggag cgcgccacgc gccagccgca ggacacgctg cgctcctacc tgcaggggct
     4681 gcagaaggcc gggttcatca cgcgcaatga gcgagcg
//
LOCUS       DASZZC010000433         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_486933,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000433 DASZZC010000000
VERSION     DASZZC010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486933"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VHE37_09195"
     CDS             complement(<1..501)
                     /locus_tag="VHE37_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT35749.1"
                     /translation="MKHAIVVEDLPSSSHWLSQALAEAFPGIEVRNAASLSAARALLR
                     AALPEIALIDLGLPDGSGVDLIAQLARSAPGCHCIVTTIFADDVHLFPALRAGAQGYL
                     LKDQPREKIVQALQGIVAGEPPLSPVIARRLLSVFTPAPSAGEEANLTPRERETLTLI
                     AKGCKLP"
     gene            699..>2010
                     /locus_tag="VHE37_09200"
     CDS             699..>2010
                     /locus_tag="VHE37_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35750.1"
                     /translation="MKARSLCLLAGLFLAAAVPAQAALTFTQSAGGPQSSAGDLYPRG
                     DGAIYSLLSDRVYKSTDGGSNWSLLGGAPPERASMLAFAGTDLYAAGVFGVLRSTDDG
                     VTWNDFNGSADTALPFTTVYQLYSTGSFLFAVGQDDNFNPLVYRSAVGGASWSNVTPP
                     GIGSNQVNSGAVTGSTLCLSVTNSGIRYTANGGDSWSSSSGVSGNIPFVAASPAAAGT
                     VYASNSGGGMFRSTNSCGAFSGIGSALTGVTGGLVTRLGVDASAHVYVASADGNVRMS
                     SNPASSAATYDLINGGSLPADTQVPVLGFAPVSGGIVAGSTGLGLNFSDGTSNWTRRG
                     NGLKGGAISALMSGPGTSIALTAGGVLSTSDSGATYTEDNNGLVLRNTGAIVRKGNTW
                     LVAAFQPSNGSGTAPPGLYRSTDAGATWTQLLAASGLPAGAGIGD"
BASE COUNT          306 a          738 c          676 g          290 t
ORIGIN      
        1 cggcagcttg cagcccttgg cgatcagcgt cagcgtctcg cgctcacgcg gcgtcaggtt
       61 ggcttcctcg ccggcggaag gcgcgggcgt gaacacgctg agcaggcgcc gcgcgatcac
      121 cggcgacagc ggcggttcgc cggccacgat gccctgcagc gcctgcacga tcttctcgcg
      181 cggctggtcc ttgagcaggt agccctgcgc gccggcgcgc agcgccggga acaggtgcac
      241 gtcgtcggcg aagatggtgg tgacgatgca gtggcagccc ggcgcgctgc gcgccagctg
      301 cgcgatcaga tccacgccag agccgtcggg caggcccagg tcgatcagcg cgatctcggg
      361 caatgccgca cgcagcagcg cgcgcgccgc cgacaggctg gcggcattgc gcacctcgat
      421 gccgggaaat gcctcggcca gcgcctggct cagccagtgc gacgacgacg gcagatcctc
      481 caccacgatg gcgtgcttca agtccggccc cccggtcgcg cgctgcgtgc gcgccggact
      541 ctgggacgca ttgttgtcgt tgtccagccc ctcctccatg ggcgcagcat gcgccgccgc
      601 ctgtagggat tcccaccccc cagatagggg atttggttta cagcgcggtg gacatagcgt
      661 ggctacagtc ctttccacgg gtcgtcgggg atcacgtcat gaaagctcgc tcgctttgct
      721 tgctggccgg tcttttcctt gctgccgccg ttccggcaca ggccgcactc actttcaccc
      781 agagcgcggg agggccgcaa agctccgccg gcgatctcta cccgcgcggc gacggcgcca
      841 tctactcgct gctcagcgac cgcgtgtaca agtccaccga tggcggcagc aactggagcc
      901 tgctcggcgg cgcaccgccc gagcgcgcca gcatgctggc ctttgccggc accgacctgt
      961 acgccgccgg cgtgttcggc gtgctgcgct ccaccgatga cggcgtgacc tggaacgact
     1021 tcaacggcag tgccgacacc gcgctgccct tcaccaccgt gtatcagctg tacagcaccg
     1081 gcagcttcct gttcgccgtg ggccaggacg acaacttcaa cccgctggtc taccgcagcg
     1141 cggtgggcgg cgcgtcgtgg agcaacgtga cgccgccggg catcggcagc aaccaggtca
     1201 acagcggcgc ggtcaccggc agcacgctgt gcctgtcggt gaccaacagc ggcatccgct
     1261 acaccgccaa cggcggcgac agctggagca gttccagcgg cgtatccggc aacatcccct
     1321 tcgttgccgc ttcgccggcc gcagcaggaa cggtgtacgc gtccaacagc ggcggcggca
     1381 tgttccgcag caccaacagc tgcggcgcct tcagcggcat cggctccgcg ctcaccggcg
     1441 tcaccggcgg actggtcacg cgcctgggcg tggatgcatc cgcgcatgtc tacgtggcct
     1501 cggccgacgg caacgtgcgc atgagcagca acccggccag ctctgccgcc acctatgacc
     1561 tgatcaacgg cggcagcctg ccggccgaca cgcaggtgcc ggtgctcggc ttcgcgccgg
     1621 tgtccggcgg catcgtcgcc ggcagcaccg ggctgggcct gaacttcagc gacggcacca
     1681 gcaactggac gcgccgcggc aacgggctca agggcggcgc catcagcgcg ctgatgtccg
     1741 gcccgggcac cagcatcgcg ctcaccgccg gcggcgtgct cagcaccagc gacagcggcg
     1801 ccacctatac cgaggacaac aacggactgg tcctgcgcaa taccggcgcc atcgtgcgca
     1861 agggcaacac ctggctggtg gcggcgttcc agcccagcaa cggcagcggc accgccccgc
     1921 cggggctgta tcgcagcacc gatgcgggcg ccacctggac gcaactgctg gcggcgtccg
     1981 gcctgccggc gggcgcgggc atcggcgacc
//
LOCUS       DASZZC010000434         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_357646,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000434 DASZZC010000000
VERSION     DASZZC010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357646"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VHE37_09205"
     CDS             complement(<1..581)
                     /locus_tag="VHE37_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT35751.1"
                     /translation="MTNNQDLVARSLAHVWQPCTQMHDHAAGLLPLVPIKSADGAWLE
                     DYDGKRYLDAVSSWWTNIFGHRHPHIVESLTRQLDAVDHVMMGGFTHGPAIELAERLC
                     KLAPPGLTRCFYADNGSSAIEVALKMSFHCWANRGQPQKKKFIALENGYHGETLGALA
                     VGDTGLYREPYAPLLMQPIVVPSPDCYGRAPGES"
     gene            complement(656..1975)
                     /gene="argA"
                     /locus_tag="VHE37_09210"
     CDS             complement(656..1975)
                     /gene="argA"
                     /locus_tag="VHE37_09210"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HVT35752.1"
                     /translation="MQSSDFVSALRGCAPYVHAHNGSVFVIAFGGEVCARPDFEPFLY
                     DLALLHSLGVKLVLVHGARTQIDRELAERNLSTPYLNGLRVTDRRALECVKAAVGSLR
                     MDIEAKLSTGLVSTPMGGARLKVATGNFVTARPVGVRDGVDHAHTGEVRRVDVDTIRD
                     ELEKDRIVLLSPIGYSPTGEIFNLRYEDVATQTAMALSADKLVYVLPSDPNTWKLADD
                     TGDAGQLSLAEAEDLLAKAPLNDDDRQHLATAISAGRGGVKRVHLVGAQTDGAILREL
                     YTRDGAGLMLYADAEYEATRDATIEDLGGILKLIKPLEDRGVLVPRSREQLELEISAF
                     NVIVRDGLVIACCALFPFPQNGMGELACVAVHPDYQRAGRANALLKRVEAEARKLGLK
                     KIFSLTTHSPHWFIEHGFTQAAISDLPVQKQRIYNYQRNSIVLVKDL"
     gene            2165..2728
                     /locus_tag="VHE37_09215"
     CDS             2165..2728
                     /locus_tag="VHE37_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HVT35753.1"
                     /translation="MSTLHYSDLGAELGRIGLPGEPAGYHGALCGALCVRQPEEIDLV
                     ALTQTEADTPHDDPAAHSLLQRLARQALDGLQDEQLSFSPLLPDDAAALGPRVRALSS
                     WCEGFLFGLSSRAGLDLDAVSEDAREIIRDFTQFTHASISVGENAEVEESAYAELVEY
                     IRVGAQLIYMELRPAPTPDPSRTKSLH"
     gene            2728..>2887
                     /locus_tag="VHE37_09220"
     CDS             2728..>2887
                     /locus_tag="VHE37_09220"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HVT35754.1"
                     /translation="MKPAANELKEYAARRARLSRLIGKRGVAIVPAAGEVVRARDTHY
                     KFRQDSDFK"
BASE COUNT          459 a          946 c          975 g          507 t
ORIGIN      
        1 gattcgccgg gcgcgcgtcc gtagcagtcg ggcgagggca cgacgatcgg ctgcatgagc
       61 agcggcgcgt aaggctcgcg gtagaggccg gtgtcgccga ccgccagcgc gcccagcgtc
      121 tcgccgtggt agccgttttc cagcgcgatg aatttcttct tctgcggctg gccgcggttg
      181 gcccagcagt ggaagctcat cttcagcgcc acttcgatgg ccgacgagcc gttgtcggcg
      241 tagaagcagc gcgtgagccc gggcggcgcc agcttgcaca ggcgctcggc cagttcgatg
      301 gccgggccgt gggtgaagcc gcccatcatc acgtgatcga ccgcatcgag ctggcgcgtc
      361 agcgactcga cgatgtgcgg atggcgatgg ccgaaaatat tggtccacca cgaactcacc
      421 gcgtcgagat agcgcttgcc gtcgtaatcc tccagccatg cgccatcggc ggatttgatg
      481 ggcaccagcg gcagcaggcc ggcggcgtgg tcgtgcatct gcgtgcaggg ctgccacacg
      541 tgggccagcg agcgcgcgac gaggtcttgg ttgttggtca ttccgggcgt atgcggggaa
      601 tcttatccgc gtgcaggctg atgagatccc tcgctggcgc tcgggatgac aggattcaaa
      661 ggtccttcac cagcacgatg gaattgcgct ggtagttgta gatgcgctgc ttctgcaccg
      721 gcaggtcgct gatcgcggct tgggtgaagc catgctcgat gaaccagtgc ggcgagtgcg
      781 tggtgagcga gaagatcttc ttcagcccca gcttgcgcgc ttccgcttcc acccgcttga
      841 gcagcgcatt ggcgcggccg gcgcgctggt aatcgggatg caccgccacg caggccagct
      901 cgcccatgcc gttctgcggg aacgggaaca gcgcgcagca ggcgatgacc aggccgtcgc
      961 gcacgatgac gttgaatgcg ctgatctcca gttccagctg ttcgcgcgag cgcggcacca
     1021 ggacgcccct gtcttccagc ggcttgatca gcttgaggat gccgcccagg tcttcgatgg
     1081 tggcgtcgcg cgtggcctcg tactcggcgt cggcgtagag catcagcccg gcgccgtcgc
     1141 gcgtgtacag ctcgcgcagg atggcgccgt cggtttgcgc gcccaccagg tgcacgcgct
     1201 tgacgccgcc gcggccggca gagatcgcgg tcgccaggtg ctggcggtcg tcgtcgttga
     1261 gcggcgcctt ggccagcagg tcctcggcct cggccagcga cagctgcccg gcgtcgccgg
     1321 tatcgtcggc cagcttccag gtgttgggat cggacggcag cacgtacacc agcttgtcgg
     1381 cggaaagcgc catcgccgtc tgcgtggcca cgtcttcgta gcgcaggttg aagatctcgc
     1441 cggtcggcga gtagccgatg ggcgagagca gcacgatgcg gtccttttcc agctcgtcgc
     1501 ggatggtgtc gacatccacc cgccgcactt cgccggtgtg tgcgtggtcg acgccgtcgc
     1561 gcacgcccac cgggcgcgcg gtgacgaaat tgccggtggc caccttcagc cgcgcgccgc
     1621 ccatcggcgt cgacaccagg ccggtggaca gcttggcctc gatgtccatg cgcagcgagc
     1681 ccaccgccgc cttcacgcat tccagcgcgc ggcggtcggt gacgcgcagg ccgttgagat
     1741 acggcgtgga caggttgcgc tccgccagtt cgcggtcgat ctgcgtgcgc gcgccgtgca
     1801 ccagcaccag cttgacgccc aggctgtgca gcagcgccag gtcgtagagg aacggctcga
     1861 agtccggccg tgcgcagacc tcgccaccga aggcgatgac gaacacgctg ccgttgtgcg
     1921 cgtgcacgta gggcgcgcag ccgcgcagcg cggaaacgaa gtcggacgat tgcatgcgcg
     1981 cgattgtacg tgcgccacct gcgcgtgcaa tttctgcctc caccgcatgc cgtttttgat
     2041 gttataaaat attttataat tattaaataa agatataatt tataacgtca atattgctgg
     2101 tgcggtcagc ggttttttgc cgtgcagcgc gaggcaaaac agcccggcgc gtataattgc
     2161 gcgcatgtcc acgctgcatt attccgacct tggcgccgaa ctcggccgca tcggtctgcc
     2221 cggcgagccg gccggttacc acggcgccct gtgcggcgca ctctgcgtgc gccagccaga
     2281 ggagatcgac ctcgtcgcgc tgacccagac cgaggccgat acgccgcacg atgatcccgc
     2341 tgcgcactcg ctgctgcagc gtctcgcgcg ccaggcgctc gacggcctgc aggacgagca
     2401 gctgagcttc agcccgctgc tgccggacga cgctgccgcg ctcggcccgc gcgtgcgcgc
     2461 gctgtcgagc tggtgcgagg gcttcctgtt cggcctgtcc agccgcgccg ggctggatct
     2521 cgacgcggtg tcggaagacg cgcgcgagat catccgcgac ttcacccagt tcacccacgc
     2581 gtccatttcg gtgggcgaga acgcggaggt ggaggagtcc gcctatgccg agctggtcga
     2641 atacatccgc gtcggcgcgc agctgatcta catggagctg cgcccagcgc ccacgcccga
     2701 tccctcacgc accaagtccc tgcactgatg aaacccgccg cgaatgaact gaaggaatat
     2761 gccgcgcgcc gcgcgcgtct ctcaagactc atcggcaagc gcggcgtggc catcgtgccg
     2821 gcagcgggcg aggtggtgcg cgcgcgcgac acgcactaca agttccgcca ggacagcgac
     2881 ttcaagt
//
LOCUS       DASZZC010000435         9382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_552092,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000435 DASZZC010000000
VERSION     DASZZC010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552092"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VHE37_09225"
     CDS             <1..1185
                     /locus_tag="VHE37_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT35755.1"
                     /translation="GIDAATGFQLVKRPDGRVLRIADLVDDDATKRDRVSASLKDPAQ
                     KNNRAYVDIIIALGMAKEGFDWIWCEHALTVGYRASLTEIVQIIGRATRDAPGKTRAR
                     FTNLIAEPDASEEAVTEAVNDTLKAIAASLLMEQVLAPRFEFKPKNADNGPTPGFDYG
                     EIGYDPDKSNVGINHQTGQVQIEIKGLVEPKSKEAARICQEDLNEVIATFIQDKTAIE
                     RGLFDEELVPEELTQVRMGKIIKDKYPDLDAEDQEAVRQHAIAALTLTQQAKLLATGG
                     ETDETSPNTALIDGVRRFAMDVRELDIDLIDRINPFGEAYAILAKTMSEESLKQVAAA
                     ISAKRANLTPDEAKELAVRAVQFKKERGRVPALDSQDAWERRMAEGAAAFMRYKKEGQ
                     YE"
     gene            1178..2380
                     /locus_tag="VHE37_09230"
     CDS             1178..2380
                     /locus_tag="VHE37_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVT35756.1"
                     /translation="MSDPDLDVLAAELAEFAEPEAKGGRPPRDERVIAGFEEIQRFVE
                     KHGRAPQHGEHHDIFERLYAVRLDRLRALPDCRALLEPLDHQGLLADSTRIAEPPGEY
                     DVEQLAAELAGAGGTEDITVLRHVRTAAEKRAAEEIADRKPCEDFEKFRPLFERVQAD
                     LKTGLRQAHPIEAGSRAIEAGDFFILDGVILYVAEVGEVLKTTAREVDRRLRLIFANG
                     TESNLLLRSLQRAFYNDPAARRLVSPESGQMSFGGELEPDDVESGTIYVLRSLSDHPY
                     VVQHRDLIHKIGVTGGKVETRIADAEHDATYLLAKVEVVATYKLAGINRARMENLFHR
                     LFASARLNITINDRFGHPVQPQEWFLVPLFVIDEAVERIKDGTVTRYVYDPKLAKLVE
                     QFSARNAD"
     gene            2440..3252
                     /locus_tag="VHE37_09235"
     CDS             2440..3252
                     /locus_tag="VHE37_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35757.1"
                     /translation="MATPRNKSATKAIRNPGDRRHRIIVNQQLIRLLGRQFSQIVARI
                     DELERELKAIPSDLSDPANKEAFQKKVENLSGWMRYYRLPLSIHLLFFVAQVGATALL
                     VDFDAVKMGQRSRLFDETLASFMAIQLSIRSLILNNTELRTLLRQALSSEKAFLDAVR
                     IDPTVLADKRMVARLSQYTLAQDAPFMRRLSSVLAQWEPAKNKDGALLRTMVYLLYRT
                     GLLYFFNEETLFHLFAKEMNPPLYSARGRKDARASLWRNVQRWKREWATRIP"
     gene            3320..4585
                     /locus_tag="VHE37_09240"
     CDS             3320..4585
                     /locus_tag="VHE37_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35758.1"
                     /translation="MQSDAVNGPDDDSAGSKRGFASAGLGLPRHEASEAGAAGSPAGA
                     PPCITAPVNSDPRILRHGIDSLYLSFPGRLSSQKDDQLRALKQCAQSEQEQLSAGAQL
                     QLADHLFEVKAHGRRRFPFVLSNNWFDIALSARDAKRLPLAHVQVSSELITSLGVEEC
                     TRRITCLIEHLGYIEDIPQVSRVDLFVDFITHLDFDAIPRHHWIKRAKNRAAYEMDDQ
                     ATGWVFGQGGDISCRLYNKTEEIKKSKKIYLPLLWAEAGWQTGETVWRLEFEFNREAL
                     QQLCVLTVLELLDKAPALWRYATTDWLRLAIPNPDDQTRARWNTHPTWQRLSDGFGID
                     APTLARVSRQRPPSDEVLFKTGLWPMTSYMAREGITDIDEGLQRYLAGLATYYRRDEG
                     SKALDEYAARKARSKARRYGTRNNRDDEE"
     gene            4798..5778
                     /locus_tag="VHE37_09245"
     CDS             4798..5778
                     /locus_tag="VHE37_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVT35759.1"
                     /translation="MSIYRRGEVWWIRFTGPDGKRVRKSAETTDKRAAQELHDRLKAE
                     AWRSRNLGEQPKSTWDDAVVQWFKEKDHKSSIEKDREIFRWADGYLRGMRLDQIDRPL
                     LFRIIEAKANETSKATANRYMALIRAVLRRACEVWEWIERVPKAPMYQVQSKRVRWAT
                     PEEAERILAELPPHQRAMMRFSLATGVRQRNVCRLEWSQVDLARCCAWIHPDQAKNKS
                     ALAVPLNTDAVSVLEEKQGEHAQYVFTYKGKPVWQVSTKGWRKALQRAGVEDFRWHDL
                     RHTWASRHAQKGTPLHVLQELGGWKSPQMVQRYAHLSTAHLLEHAQRIAM"
     gene            complement(5882..5958)
                     /locus_tag="VHE37_09250"
     tRNA            complement(5882..5958)
                     /locus_tag="VHE37_09250"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5922..5924),aa:Met,seq:cat)
     gene            complement(6011..6811)
                     /locus_tag="VHE37_09255"
     CDS             complement(6011..6811)
                     /locus_tag="VHE37_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT35760.1"
                     /translation="MKDFHGKVALVTGASSGIGEAIALALARAGATVVLTGRDRERLA
                     QVEQKCIAAGGQAWSHAAEVTDAQQMHALADAVHARHEAIDILVNNAGVVMAGLVIET
                     EPADWRRLHDINVMGVVHGCQAFVPKMIARGRGGHVINMGSASSIVGTPGMGTYCATK
                     FAVMGLSESLRAELKRARIGVSVVCPGYVDTPIAGKVKIAGSLATDRRRAAIADRFKR
                     NSVTAEQVAERTLGAIRTNKAVVTVGRDAAAAHFLKRWAPGLLARYAK"
     gene            complement(6819..8180)
                     /locus_tag="VHE37_09260"
     CDS             complement(6819..8180)
                     /locus_tag="VHE37_09260"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT35761.1"
                     /translation="MNRTHRIATFAGACALAFGAPLAAKAPADQVSKLGQELTPVGAL
                     KAANKDGTIPEWTGGMTTLPASFNNYKPGDYYPDPFPDDKPQFRITHDNYKQYADRLS
                     AGTAWLLERYPDYSLAVYPTRRTALFPDALYAATKANAASATLAGEDALKDASLGFPF
                     PIPQNGAEAIWNHKVRYRGDSVQQSGTLFVVARSGKYERADYTTSAQFVYGNVKKPGS
                     LSDNIIQQVLRKEVSPPRLAGGMTLVWDHLDGTRDAWQYSPGSNRIRQAPIVAFDNPI
                     NGSDGLQNVDQADMFNGSLQRYSWKLVGKKELYIPYNNYRMIRPELKYADLIAPTHLN
                     PAHPRYELHRVWVVEATLKPGQGNVYKKRVFYIDEDSWTITAVDCYDNRDTLWRYQEG
                     FVIPLVVDKAVVAAPLIVYDLPSGRYVVNNLPNEQGYVAKFNLPFPAGYFTPQNLQKM
                     GRN"
     gene            complement(8200..>9382)
                     /locus_tag="VHE37_09265"
     CDS             complement(8200..>9382)
                     /locus_tag="VHE37_09265"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT35762.1"
                     /translation="DNLVGTEERTQGNAFLLSSAGRTVRPGPEQDARKQGQYGITLNY
                     LATWLNNSSFSLSFLHLHSRFPFVNFISAIKGCASDATNQAEALASCRGFNGPTHLGV
                     EPIPVDTVKFFISYPEDINALGGSFSTNLGAVAWTGEIVFRPNQPLQVDPTDLGFAAL
                     QPVFPGPNINFGVVTLPSRRTATPDYVETIYRHETVGPQQVIRGYERMHTLSYNTSFL
                     LLNGASENPFGSDQVTSLLELGAFQVMGMPGLDQLQFAAPGTQFHHSAGVDSTGAPTV
                     EQTQTSAQWRLNPHYQDGGFATAFSWGYRILEQLSYEDVLPNLRLTPQLSWSHDVGGI
                     SPIPTGEFVAGRKQAMVGLAAQYLNDFGAALRYNWYFGGGLANGLSDRDNLQLSFSYD
                     F"
BASE COUNT         1807 a         2836 c         3031 g         1708 t
ORIGIN      
        1 ggcatcgacg ccgcgaccgg tttccagctc gtcaagcggc ccgacggccg ggtactgcgc
       61 atcgccgatc ttgtcgatga cgacgcgaca aagcgcgacc gcgtgtccgc ctccctcaaa
      121 gacccggcgc agaagaacaa cagggcctac gtggacatca tcatcgcgct cggcatggcg
      181 aaggaaggct tcgactggat ttggtgcgag cacgccctga ccgtcggcta tcgcgccagc
      241 ctgaccgaaa tcgtgcagat catcggtcgt gcgacccgcg acgcgccggg caagacccgc
      301 gcacgcttca ccaacctgat cgcagagccg gatgcttccg aggaagctgt caccgaggcc
      361 gtgaacgaca ccttgaaggc catcgccgct agccttctga tggagcaggt cttggccccg
      421 cgcttcgagt tcaagcccaa gaacgccgac aatggcccga cgcccggctt cgactacggc
      481 gagatcggct acgacccgga caagtccaac gtcggtatca accaccagac aggacaggtg
      541 cagatcgaga tcaaggggct cgtcgaacct aagagcaagg aagctgcgcg tatctgtcag
      601 gaagacctga atgaggtgat cgccaccttc attcaggaca agaccgccat cgagcgcggc
      661 ctatttgacg aagaactggt gcccgaagaa ctgacgcagg tccgcatggg caagattatc
      721 aaggataagt accccgacct tgacgcggaa gaccaggaag ccgtgcgcca gcacgccatc
      781 gctgccctga cgctgaccca gcaggcgaag ctgcttgcga cgggtggaga aaccgacgag
      841 acatccccca ataccgccct gatcgacggc gtgcgtcgct ttgcgatgga tgtgcgcgag
      901 ctggatatcg atctcatcga ccgcatcaac ccgttcggcg aagcctacgc cattctcgcc
      961 aagacgatga gcgaggaaag tctgaagcag gttgcggcgg caatttccgc caagcgtgcc
     1021 aacctgacgc cggacgaggc gaaggaactt gccgttcgtg ccgtccagtt caagaaggag
     1081 cgcggacgtg tgcctgcgct cgactcgcag gatgcgtggg agcgacgcat ggccgaaggt
     1141 gcagccgcgt tcatgcgcta caaaaaggag gggcagtatg agtgaccccg acctggacgt
     1201 actggcggcg gagcttgcag agtttgctga gccggaggcg aagggcggac gcccacctcg
     1261 ggatgagcgc gtcatcgcgg ggttcgagga aatccagcgt tttgtcgaaa agcacggtcg
     1321 tgcgccgcaa catggggagc accacgacat cttcgagcgt ttgtacgctg tgcgcctcga
     1381 ccgtctgcgg gcgctccccg actgccgcgc ccttcttgag cccctggacc atcaggggtt
     1441 gcttgccgat tcgacgagga ttgcagagcc ccccggcgaa tacgacgtag agcaactcgc
     1501 tgcggaactg gcgggcgctg gcgggactga ggacatcacc gtccttcgcc atgtccgtac
     1561 cgccgccgag aaacgcgccg ccgaggagat tgccgatcgc aagccatgcg aagacttcga
     1621 gaagttcagg ccactgttcg agcgtgtgca agccgacctc aagacgggct tgcgccaagc
     1681 gcatcccatt gaggcgggca gccgagcgat tgaagctgga gacttcttta ttcttgacgg
     1741 cgtcatcctt tacgtcgccg aggtaggcga ggtgttgaaa acgacagcta gggaagtgga
     1801 tcgacgccta cggctgattt tcgccaatgg caccgagagc aatctattgc tgcggtcact
     1861 tcaacgcgcg ttctacaacg atcccgccgc gcggcggctg gtctctccag aaagcgggca
     1921 gatgtccttc ggcggcgagt tggagccgga cgacgttgag agcggaacaa tctatgtcct
     1981 gcgctcgctg tccgatcatc cctatgtggt gcagcaccgt gatcttatcc acaagattgg
     2041 cgtaactggc ggcaaggtgg agacgcgcat cgcggacgcc gagcatgacg caacgtacct
     2101 cctggcgaaa gttgaggtgg ttgccactta caagctggcg ggcatcaatc gcgcgcggat
     2161 ggaaaacctg tttcacaggc tgtttgcgtc cgcgcggctg aatatcacca tcaacgaccg
     2221 cttcggccat cccgtgcagc cgcaggaatg gtttctcgtt ccgctgttcg ttattgacga
     2281 agctgtcgag cggatcaagg acggcaccgt cacccgctac gtctatgacc cgaagctggc
     2341 gaaacttgtg gagcaatttt cagcgcggaa cgcagattga atgtgagatg cctccctcgg
     2401 agttcgacaa tttagaaaga gggtttgtgc gagttagcaa tggcaacgcc taggaataag
     2461 agcgcgacca aggcgatcag aaatcccggg gatcggcgcc atcgaatcat cgtcaaccaa
     2521 caactcatcc gcctgttggg ccggcaattc agtcaaattg tcgccaggat cgacgaactc
     2581 gaacgtgaat taaaagcgat cccgtccgat ctgagcgacc cagcgaataa ggaagccttc
     2641 cagaaaaaag ttgaaaacct ctctggatgg atgaggtact accgcttgcc gctgtccata
     2701 catctattgt tcttcgtggc tcaggttgga gccacggcgc tactggtgga ttttgacgct
     2761 gtcaaaatgg gtcagcggtc acggctcttc gacgaaacac tggcttcgtt catggcgatc
     2821 cagctttcga tccgcagttt gatccttaac aacaccgagt tgcggaccct tctacgacaa
     2881 gcattatcga gcgaaaaggc gttccttgac gctgttcgca tagatccaac cgttctcgca
     2941 gacaaaagga tggttgcccg tctctctcag tacaccttgg ctcaggacgc gccgtttatg
     3001 aggcgtttga gttccgttct cgcgcagtgg gagccagcga agaacaagga tggcgctttg
     3061 ctgcgcacga tggtctacct gttgtatcgc acgggccttc tgtacttctt caacgaggaa
     3121 accttgttcc accttttcgc gaaagaaatg aatccaccac tgtacagcgc caggggacgc
     3181 aaggacgcgc gggccagtct gtggcggaat gtgcagcgat ggaaacggga gtgggcgaca
     3241 agaattccgt aagaattgtc gtggcgattg ctgcggttcg atcaatattg tttgggctcc
     3301 tcaacaacag gagttcagca tgcaaagcga cgcagtcaac gggccagacg atgattccgc
     3361 cggcagcaaa cgaggttttg cgagcgccgg cctcggcttg ccgaggcacg aggcatccga
     3421 ggccggcgct gccgggtctc cagcgggtgc gccgccctgt atcacagcac ccgtaaacag
     3481 cgatccccgg attctgcgtc acggcatcga tagcctgtac ctatcttttc cgggcaggct
     3541 ttcgagccaa aaggacgatc agctgagggc attgaagcaa tgcgctcagt ctgagcagga
     3601 acagctttcc gctggagcgc agctgcagct ggccgaccac ctgttcgagg tcaaggccca
     3661 cggccgccga cgcttcccgt ttgtcctctc caataactgg ttcgacatcg ctctttccgc
     3721 gcgtgatgcc aagcgcctgc cgttggcgca cgtgcaggtt tccagcgaac tcatcacctc
     3781 tcttggcgtt gaggaatgca ctcggcgaat cacctgcttg atcgaacacc tcggctatat
     3841 cgaggacatt ccccaggtca gccgcgtgga cctcttcgtc gacttcatca cgcacctgga
     3901 cttcgacgcc atcccgcgcc atcactggat caagcgcgcg aagaatcgcg ccgcttacga
     3961 aatggacgat caggccaccg gctgggtctt cggccaaggc ggtgacatct cctgtcgcct
     4021 gtacaacaaa accgaggaaa tcaaaaaaag caaaaaaatc tatttgcctc tgctctgggc
     4081 agaagccggc tggcagacag gggagacggt gtggcgcctg gaattcgaat tcaaccgcga
     4141 agcgctgcag caactttgcg ttctgaccgt cctggagctg ctcgataagg ctccggcgct
     4201 ctggcgctac gccactaccg actggctgcg tctggcgata cccaatcccg acgatcaaac
     4261 ccgcgcccgc tggaacaccc atccgacctg gcagcggctg tccgatggct ttggcatcga
     4321 cgccccaacc ctggctcgcg tatcgcggca acgcccgccc agcgacgaag tgctgttcaa
     4381 aaccgggtta tggccgatga cctcctacat ggcgcgcgaa ggcatcaccg acatcgacga
     4441 aggcttgcag cgctaccttg ccgggcttgc gacctactac cgccgcgatg agggcagcaa
     4501 ggcgcttgac gagtacgcgg cacgcaaagc gcgcagcaag gcccgccgct acggcacccg
     4561 gaacaatcgc gatgatgagg aataaatcat gcgccgctac cgccttgccg acgcggcggc
     4621 ttacctctgc gttgcgccgc gaaccttgct caagctcgcc cgccgcggcg acgtgcgcgg
     4681 cagcaagccc ggcaagtgct gggtattcct cgaagacgat cttgtcgcct acttgcggga
     4741 cggctacatt tccgcccggc acatgccgcg tcgtggctcc aagctggaga ttggagcatg
     4801 tcgatctata gacgcggtga agtatggtgg atcaggttta ccggccccga cggcaagcga
     4861 gtacgaaagt ctgctgagac taccgacaag cgcgccgcac aggaacttca cgaccggctg
     4921 aaagccgaag cgtggcgctc ccgcaatctg ggcgagcagc cgaaatccac gtgggacgat
     4981 gcggtggtgc agtggttcaa ggaaaaggac cacaaatcat ccatcgagaa ggaccgggag
     5041 atttttcgct gggcggatgg ttatctgcgc ggcatgcggc tggatcaaat cgaccggccg
     5101 ctgctgtttc ggatcatcga agccaaagcg aacgaaacgt ctaaggcgac cgcaaaccgc
     5161 tacatggcgc tgatccgggc cgtactgcgg cgagcgtgcg aagtatggga atggatcgag
     5221 cgcgtgccca aggctccgat gtatcaggtg cagtccaagc gcgtgcgctg ggcgacaccc
     5281 gaagaggccg agcggatact cgccgaactg ccaccccacc agcgcgccat gatgcggttc
     5341 tcgctcgcga cgggcgttcg tcagcgcaat gtctgccggc tggagtggtc gcaagtggat
     5401 ctcgcgcgat gttgcgcctg gatacacccg gaccaagcca agaacaaatc ggcgctcgcc
     5461 gtgccgctca acaccgacgc ggtttcggtc ctggaagaga agcaaggcga gcacgcacag
     5521 tacgtattca cgtacaaggg caagccggtt tggcaggtca gcaccaaagg ctggcgcaag
     5581 gcgttgcaaa gggcgggcgt ggaggacttc cgctggcacg acttgcgaca cacctgggca
     5641 tcgcgccacg cgcagaaagg tacgccgcta cacgtgttgc aggagctggg cggctggaaa
     5701 tcgccgcaga tggtccagcg ctatgcacat ctatcgacgg cgcatctgct ggaacatgcg
     5761 cagcggattg cgatgtgata cggtgttttt agaataggga aagtgtgcca gccagtggca
     5821 caattttggc acagttggtt aaaaaatgac caacctcagt tttcgtaagt gcctgaaaaa
     5881 ttggtggtga tgagtggaat cgaaccaccg acctcagcat tatgagtgcc gcgctctaac
     5941 cgtctgagct acatcaccac gtcgggggcg cgcattctaa gtgctggcgc cggtggcgta
     6001 aacgggcgcg ctatttggcg tagcgggcca gcaatccggg cgcccagcgc ttgaggaagt
     6061 gggccgccgc ggcatcgcgg ccaacggtga ccacggcctt gttggtgcgg atggcgccca
     6121 gcgtgcgctc ggccacctgt tccgcggtga cggaattgcg cttgaagcga tcggcaatcg
     6181 ctgcgcggcg gcggtcggtg gccagcgagc cggcgatctt caccttgccg gcgatcggcg
     6241 tatcgacgta gccggggcaa acgacgctga cgccgatgcg cgcgcgtttg agttcggcgc
     6301 gcagcgattc cgacaggccc atcaccgcga acttggtggc gcagtaagtg cccatgccgg
     6361 gcgtgccgac gatgctggag gcggagccca tgttgatcac gtggccgccg cggccgcgcg
     6421 cgatcatctt gggcacgaag gcctggcagc cgtgcaccac gcccatcacg ttgatatcgt
     6481 gcagccggcg ccagtcggcg ggctcggttt cgatcaccag tccggccatg accacgccgg
     6541 cgttgttgac caggatgtcg atggcttcgt gccgcgcgtg caccgcgtcg gccagtgcat
     6601 gcatctgctg cgcgtcggtt acctcggccg cgtggctcca ggcctggccg ccggcggcaa
     6661 tgcacttctg ctcgacctgg gccagccgct cgcggtcgcg cccggtgagt acgacggtgg
     6721 caccggcccg ggccagtgcc agcgcgatgg cttcgccgat gccgctcgat gcgccggtga
     6781 ccagcgcaac cttgccgtgg aagtctttca tgcgcgcctc agttgcgtcc catcttctgc
     6841 aggttctgcg gagtgaagta gccggccggg aagggcaggt tgaacttggc cacgtagccc
     6901 tgctcgttgg gcaggttgtt gaccacgtag cggcccgagg gcaggtcgta cacgatcagc
     6961 ggcgcagcga ccacggcctt gtccaccacc agcgggatga cgaagccttc ctggtagcgc
     7021 cacagcgtgt cgcggttgtc gtaacagtcc accgcggtga tggtccagct gtcctcgtcg
     7081 atgtagaaca cgcgcttctt gtagacgttg ccctggcccg gcttgagcgt ggcctcaacc
     7141 acccagacgc ggtgcagctc gtagcgcggg tgcgccgggt tgagatgcgt gggcgcgatc
     7201 aggtcggcgt atttcagctc cggccggatc atccggtaat tgttgtacgg gatgtaaagt
     7261 tccttcttgc cgaccagctt ccacgaatag cgctgcagcg aaccgttgaa catgtcggcc
     7321 tggtcgacgt tctgcaggcc gtcggaaccg ttgatgggat tgtcgaacgc gacgatcggc
     7381 gcctggcgga tgcggttgga gccgggcgag tactgccagg cgtcgcgcgt gccgtcgagg
     7441 tgatcccaca ccagggtcat gccgccggcg agccgcggcg gcgacacttc cttgcgcagc
     7501 acctgctgga tgatgttgtc cgacagcgag ccgggcttct tcacgttgcc gtacacgaac
     7561 tgcgcgctgg tggtgtagtc ggcgcgctcg tacttgccgc tgcgcgccac cacgaacagc
     7621 gtgccgcttt gctgcaccga atcgcctcgg tagcgcacct tgtggttcca gatcgcctcg
     7681 gcgccgttct gcggaatcgg gaagggaaaa cccaggctgg catccttcag cgcgtcttcg
     7741 ccggccagcg tggcactggc ggcgttggcc ttggtagcgg catacagcgc gtcggggaac
     7801 agcgcggtgc ggcgcgtcgg gtacaccgcg agcgaataat ccgggtagcg ttcgagcagc
     7861 cacgccgtgc cggcagagag ccggtccgcg tactgcttgt agttgtcatg cgtgatgcgg
     7921 aactgcggct tgtcgtccgg gaacggatcg gggtagtagt cgcccggctt gtagttgttg
     7981 aacgaggccg gcagcgtggt catgccgccg gtccactcgg ggatggtgcc gtccttgttg
     8041 gcggccttga gcgcaccgac cggcgtgagt tcctggccga gcttgctcac ctggtcagcg
     8101 ggcgccttgg cggccagcgg cgcgccgaag gccagcgcgc aggcgccggc gaaggttgcg
     8161 atgcgatgcg tgcggttcat gcgcttactc cgtgcatgct cagaagtcgt agctgaagct
     8221 cagttgcagg ttgtcgcggt ccgacaggcc gttggccagg ccgccgccga agtaccagtt
     8281 gtagcgcagc gcggcgccga agtcgttgag gtactgcgcg gccaggccga ccatggcctg
     8341 cttgcgaccg gcgacgaatt cgccagtggg gatcggcgag atgccgccga cgtcgtgcga
     8401 ccacgacagc tgcggcgtca ggcgcaggtt gggcagcacg tcttcgtagc tcagctgctc
     8461 gaggatgcgg taaccccacg aaaacgcggt ggcgaagccg ccgtcctggt agtgcgggtt
     8521 gagccgccac tgcgcgctgg tctgggtctg ctccaccgtc ggcgcgccgg tgctgtcgac
     8581 gccggcgctg tgatggaact gggtgcccgg cgcggcgaac tgcagctggt ccaggcccgg
     8641 catgcccatc acctggaatg cgccgagttc gagcagcgag gtcacctggt cggagccgaa
     8701 cgggttctcg ctggcgccgt tgagcagcag gaacgaggtg ttatacgaca gcgtgtgcat
     8761 gcgctcgtag ccgcggatca cctgttgcgg cccgaccgtt tcatggcggt agatggtttc
     8821 cacgtagtcc ggcgtggcgg tgcggcgcga aggcagcgtg accacgccga agttgatgtt
     8881 ggggcccggg aacaccggct gcagcgcggc gaagccgagg tcggtcgggt ccacctgcag
     8941 cggctggttg gggcgaaaca cgatctcgcc ggtccacgcc accgcgccca ggttggtgga
     9001 aaagctgccg cccagcgcgt tgatgtcctc cgggtagctg atgaagaact tcaccgtgtc
     9061 gaccgggatc ggctccacgc ccaggtgcgt cggcccgttg aagccgcggc acgaggccag
     9121 cgcctcggcc tggttggtgg cgtctgacgc gcagcccttg atcgccgaga tgaagttcac
     9181 gaacgggaag cggctgtgca ggtgcaggaa gctgagcgag aagctgctgt tgttcagcca
     9241 ggtggccagg tagttgaggg tgatgccgta ctggccctgc ttgcgcgcgt cctgctcggg
     9301 gccgggccgc acggtgcggc cggcgctcga cagcaggaaa gcattgccct gggtgcgctc
     9361 ctcggtgccg accaggttgt cc
//
LOCUS       DASZZC010000436         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_487271,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000436 DASZZC010000000
VERSION     DASZZC010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487271"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VHE37_09270"
     CDS             complement(<1..299)
                     /locus_tag="VHE37_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HVT35763.1"
                     /translation="MYALRLRFARWVLAHRAGGIAAFALITAFFAAQLPQVQLRTIFS
                     DLLPRDDPFVQVYRDHPNFGSPLTVLAMIRRTDGDIYDAQTIDKIWRFTRDIDLAP"
     gene            complement(471..1853)
                     /locus_tag="VHE37_09275"
     CDS             complement(471..1853)
                     /locus_tag="VHE37_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT35764.1"
                     /translation="MNLRRYDRGYSRRHFLKQLAAGAGGAGVLMPLHRAIAASGDVSK
                     AYPDELLSIENYTRGKIKTGDTIDASNVDVVKELMEPIRYEQIAHQGRKLKIARTTTD
                     VMRLSPWDYIEATLRNAGKAKFDAKGNVVNGADGQPWIGGNPFPDPKDGLELFAAQTL
                     SWGRHDASFYAFNEYDLDASGEVKFLYTGGWAELSPVARVTLDPKPYWPEHKDKLRFQ
                     SVFFLSPQNLRGTSFLNVWDYDHSTFPSLYGYVAEFRRIRQFPTDQRFEPLVPGSSLY
                     LSDAWAAGDPLHTWGNYRIVGRGPFLAGVSGGWNADHPNWEHTTHGGPKGKSFWDTTV
                     ELVPEAIIVEAEPVKFPRAPVSKKRVWFDARNQTVIGMVSFDRRGQPYRSFDGAYSLY
                     DNGSKRVMDGKHPYWSWAHVTAADIQTGKITRLEQVKSIEKIHVSGANDPQLYERYLT
                     QGALMKLGAV"
     gene            complement(1923..>3127)
                     /locus_tag="VHE37_09280"
     CDS             complement(1923..>3127)
                     /locus_tag="VHE37_09280"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT35765.1"
                     /translation="WQFTDELLADGFVQQFQPTVYGNPNTQYNVIPVGFTVHDMYYAE
                     GYDKKWNYGLRFKGNYGQWGFQAMATRRYNPDGVFRWTASGVDRNLPCSNLLANTIAC
                     RVELTLNGEGKRSGALLAQSAFEVAPGGVYSANEWFNYAAKVRLQAIDGLNASIVDFP
                     ATAELFATPVDYQSTCADQNCQAWNELNTFFIAAGGSLRGHIAREYFAENTYALGGSY
                     VTEGEPGSILDQLIINLESSYTPKRTYTPLTLTTKGYPQFNSSVTALVMEKYYRFTDA
                     FPATYFVFQYMHRNVDDLFGRLLSGYGGTETTQAPGVHNADYVVLAAQQPFPQDVYRA
                     AFAMLVDPRGSILVQPGLRWKPNREWQVDGFYTYINGALGGANPNNTLLSTLDFADEF
                     TIRLTYQF"
BASE COUNT          494 a          982 c         1093 g          558 t
ORIGIN      
        1 ggcgccaggt caatgtcgcg ggtgaagcgc cagatcttgt cgatggtctg cgcatcgtaa
       61 atatctccat cggtgcggcg gatcatcgcc agcacggtca gcgggctgcc gaagttggga
      121 tggtcccggt acacctgcac gaacgggtcg tcgcgcggca gcaggtcgga gaagatggtg
      181 cgcagctgca cctgcggcag ctgcgcggcg aagaacgcgg tgatcagcgc aaaggcggca
      241 atgccgcccg cgcgatgcgc cagcacccag cgggcgaagc gcaggcgcag ggcgtacatg
      301 gatttattcg cgcgccttgc ccctctccct ctgggagagg ggcaggggtg aggggcggag
      361 ccggatgaaa caagcgacgg ttgtttcccg cccagcctcc ctcagccggc gctgcgcgcc
      421 gacctctccc gatcggagag gcaggaagtc cggctgcagt attggcagcg tcacaccgcg
      481 cccagcttca tcagcgcgcc ctgcgtcagg tagcgctcgt acagctgcgg atcgttggcg
      541 ccggacacgt ggatcttctc gatcgacttc acctgctcca gccgcgtgat cttgccggtc
      601 tggatgtcgg cggcggtgac atgcgcccac gaccagtacg ggtgcttgcc gtccatcacg
      661 cgcttgctgc cgttgtcgta caggctgtac gcgccgtcga acgagcggta cggctggccc
      721 cggcgatcga acgacaccat gccgatcacc gtctggttgc gcgcgtcgaa ccacactctc
      781 ttcttcgaca ccggcgcgcg cggaaacttc accggctcgg cctcgacgat gatcgcctcc
      841 ggcaccagct ccacggtggt gtcccagaac gatttgccct tgggtccccc gtgcgtggtg
      901 tgctcccagt tcgggtggtc cgcgttccag ccgcccgaca cgccggcgag gaacggcccg
      961 cggccgacga tgcggtaatt gccccaggtg tgcagcgggt cgcccgcggc ccaggcgtcg
     1021 gacaggtaca gcgacgagcc cggcaccagc ggctcgaagc gctggtcggt ggggaactgg
     1081 cggatgcggc ggaactcggc cacgtagccg tacagcgacg gaaaggtgct gtggtcgtag
     1141 tcccacacgt tgaggaacga ggtgccgcgc aggttctgcg gcgagaggaa gaacaccgac
     1201 tggaagcgca gcttgtcctt gtgctcgggc cagtacggtt tcggatcgag cgtgacgcgc
     1261 gccaccggcg acagctcggc ccagccgccg gtgtagagga acttcacctc gccgctggca
     1321 tccaggtcgt attcgttgaa ggcgtagaac gaggcgtcgt gccggcccca cgacagcgtc
     1381 tgcgcggcga acagctccag cccgtccttg ggatcgggaa acggattgcc gccgatccac
     1441 ggctggccat cggcgccgtt gaccacgttg cccttggcat cgaacttcgc cttgccggcg
     1501 ttgcgcaatg tcgcttcgat gtaatcccac ggcgacaggc gcattacgtc ggtggtggtc
     1561 ctggcgatct tcagcttgcg gccctggtgc gcgatctgct cgtagcggat cggctccatc
     1621 agctccttca ccacgtcgac attgctggcg tcgatggtgt cgccggtctt tatttttccg
     1681 cgcgtgtagt tctcgatcga cagcagctca tcgggatacg ccttgctcac gtcgccgctc
     1741 gcggcgatgg cgcggtgcag cggcatcagc acgccggcgc cgcccgcgcc ggcggccagc
     1801 tgcttgagga aatgccggcg gctgtagccg cggtcgtagc ggcgcaggtt catgcgtagg
     1861 aataagaggc ccggcggccc gtgcaggcgc cgccgggagc gggggagcgg ttgcgcgctg
     1921 cgctagaact ggtaggtgag gcggatggtg aactcatcgg cgaaatcgag cgtggacagc
     1981 agcgtgttgt tcgggttggc gccgccgagc gcgccgttga tgtaggtgta gaagccgtcc
     2041 acctgccact cgcggttcgg cttccagcgc aggccgggct gcaccaggat gctgccgcgc
     2101 ggatcgacca gcatggcaaa ggcggcgcgg tacacgtcct gcgggaacgg ctgctgcgcg
     2161 gccagcacaa cgtagtcggc gttgtgcacg ccgggcgcct gcgtggtttc ggtgccgcca
     2221 tagcccgaca gcaggcggcc gaacaggtcg tccacgttgc ggtgcatgta ctggaacacg
     2281 aagtacgtcg ccgggaaggc atcggtgaag cggtagtact tctccatcac cagcgcggtg
     2341 accgaggagt tgaactgcgg atagcccttg gtggtgaggg tgagcggggt gtaggtgcgc
     2401 ttgggcgtgt agctgctctc caggttgatg atcagctggt cgaggatgct gcccggctcg
     2461 ccctcggtaa cgtagctgcc gccgagtgcg taggtgttct cggcgaaata ctcgcgcgcg
     2521 atatgtccgc gcagcgagcc gccggcggcg atgaagaagg tgttgagctc gttccacgcc
     2581 tggcagttct ggtcggcgca ggtgctctgg tagtccaccg gtgtggcgaa cagttccgcc
     2641 gtcgccggga aatccacgat cgacgcgttg aggccgtcga tcgcctgcag gcgcaccttg
     2701 gccgcgtagt tgaaccactc gttggccgag tacacgccgc ccggcgccac ttcgaatgcg
     2761 ctctgtgcca gcagcgcgcc ggagcgcttg ccctcgccgt tgagcgtgag ctccacgcga
     2821 caggcgatgg tgttggccag caggttggag cacggcaggt tgcggtccac gcccgacgcg
     2881 gtccagcgga acacgccgtc ggggttgtag cggcgcgtgg ccatggcctg gaaaccccat
     2941 tgcccgtaat tgcccttgaa gcgcaggccg tagttccact tcttgtcgta accctcggcg
     3001 taatacatgt cgtgcacggt gaagccgacc gggatcacgt tgtactgcgt gttcgggttg
     3061 ccgtagaccg tgggctggaa ttgctgcacg aagccgtcgg ccagcagctc gtcggtgaac
     3121 tgccacg
//
LOCUS       DASZZC010000437         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1062742,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000437 DASZZC010000000
VERSION     DASZZC010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062742"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VHE37_09285"
     CDS             complement(<1..180)
                     /locus_tag="VHE37_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVT35766.1"
                     /translation="MSSNLRTILVTNALPYANGPLHLGHMVGYIQADIWVRYQRMRGH
                     EVTYVCADDAHGTPIM"
     gene            250..1335
                     /gene="apbC"
                     /locus_tag="VHE37_09290"
     CDS             250..1335
                     /gene="apbC"
                     /locus_tag="VHE37_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HVT35767.1"
                     /translation="MATLSDEQLRDALDAYVDPLIGRGLAAAGAVRQAGVADGKAQVQ
                     IELGFPAEGYARELAEKIAQHLHPQLGDLELSVRIGWNIQSHAVQKNLKPLPGIRNII
                     AVASGKGGVGKSTVAVNLALALRAEGARVGILDADIYGPSQPRMLGSSAKPTSPDGRL
                     MNPIVAHGIQAMSIGFLIDESEPMIWRGPMVTQALAQLINETKWQDLDYLVVDLPPGT
                     GDIQLSLSQRIPVSGAVIVTTPQDIALLDARKGLAMFRKVDVAVLGVVENMSTHVCSS
                     CGHEEHIFGAGGGARLAAQYGVELLGSLPLDIRIREQADGGNPTVAAEPESELALRYR
                     SIARLAAARLAYGTAQGAFPTISSSDD"
     gene            1343..>1531
                     /locus_tag="VHE37_09295"
     CDS             1343..>1531
                     /locus_tag="VHE37_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35768.1"
                     /translation="MADWRNVLLAVLVLAGIGGAWHHWSTRPVQIVTPGQLAPDEPEQ
                     RPVDGAAQASRWGEFQVTP"
BASE COUNT          258 a          506 c          531 g          236 t
ORIGIN      
        1 catgatcggc gtgccgtgtg catcgtcggc gcagacgtag gtgacctcgt gcccgcgcat
       61 gcgctggtag cgcacccaga tgtcggcctg gatgtagccc accatgtggc ccaggtgcag
      121 cgggccgttg gcgtagggca gggcgttggt gaccaggatc gtccgaagat tgctgctcat
      181 tttcatcgct gcttttttga gcatgaatta tgacatgcag accacgatgc taaacttggc
      241 acctccccga tggccacgct ctccgacgaa caactgcgcg acgcgctcga cgcctatgtc
      301 gatccgctga tcggccgcgg gctggccgcc gccggcgcgg tgcggcaggc cggggtggcg
      361 gacggcaagg cacaggtgca gatcgaactc ggcttcccgg ccgaaggata tgcgcgcgaa
      421 ctcgcggaga agattgcgca gcacctgcat ccgcagctgg gcgacctgga actcagcgtg
      481 cgcatcggct ggaacatcca gtcgcacgcg gtgcagaaga acctgaagcc gctgcccggc
      541 atcaggaaca tcatcgccgt ggcttcgggc aagggcggcg tcggcaagtc gaccgtcgcg
      601 gtgaacctgg cgctggcgct cagggccgaa ggcgcgcgcg tgggcatcct cgacgccgac
      661 atctacggcc ccagccagcc gcgcatgctg ggcagcagtg ccaagccgac ctcgccggac
      721 ggccgcctga tgaacccgat cgtggcgcac ggcatccagg cgatgagtat tggatttctc
      781 atcgacgaaa gcgagccgat gatctggcgc ggccccatgg tcacgcaggc gctggcgcag
      841 ctgatcaacg aaacaaaatg gcaggacctc gattacctgg tggtcgacct gccgccgggc
      901 actggcgata tccagctatc gctgtcccag cgcatcccgg tgagcggcgc ggtcatcgtc
      961 accacgccgc aggacatcgc cctgctcgac gcgcgcaaag gcctggccat gttccgcaag
     1021 gtggacgtgg cggtgctcgg cgtggtggag aacatgagca cgcatgtctg ctccagctgc
     1081 ggccacgagg aacacatctt cggcgccggc ggcggcgcgc ggctggcggc gcaatacggc
     1141 gtggaactgc tcggctcgct gccgctggac atccgcatcc gcgagcaggc cgacggcggc
     1201 aacccgaccg ttgccgccga gccggaatcc gaactggcgc tgcgttaccg ctccatcgcg
     1261 cggttggcgg cggcgcgcct ggcgtacggc accgcgcagg gcgcgttccc caccatcagc
     1321 agcagcgacg actgatcgtc acgtggccga ttggcgcaat gttctgctcg cggtcctggt
     1381 actggccggc atcggcggcg cctggcatca ctggagcacg cggccggtgc agatcgtcac
     1441 accgggtcag ctggcgccgg acgagcccga acagcggccg gtcgacggtg cggcgcaggc
     1501 ctcgaggtgg ggcgagttcc aggtcacgcc g
//
LOCUS       DASZZC010000438         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_358050,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000438 DASZZC010000000
VERSION     DASZZC010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358050"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHE37_09300"
     CDS             <1..189
                     /locus_tag="VHE37_09300"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HVT35769.1"
                     /translation="LTALLSGKLALLWLLPVLGYGFAWVGHFYFEHNRPATFKYPFYS
                     LASDFVMYKDILTGKIRF"
     gene            192..575
                     /locus_tag="VHE37_09305"
     CDS             192..575
                     /locus_tag="VHE37_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35770.1"
                     /translation="MDGPALIAGLETGSLPPAQFSHENHVRAARQLLLAEPLPQAAHR
                     FAELLRNYVGGIGAQDKFHLTLTLAFMHLIHQRMRAGDSWDGFRARHPELFRDAAALI
                     ARHYSRERIAAGRSVFIEPDGLPLP"
     gene            601..>1929
                     /gene="mnmG"
                     /locus_tag="VHE37_09310"
     CDS             601..>1929
                     /gene="mnmG"
                     /locus_tag="VHE37_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HVT35771.1"
                     /translation="MNTQQSYDVIVIGGGHAGTEAALAAARMGARTLLLTQNIETLGQ
                     MSCNPAIGGIGKGHLVREIDAMGGLMAHAADHAGIQFRTLNASKGPAVRATRAQADRA
                     LYKKFVRSALENQPHLALFQQEVDDLVVEGGCVTGVVTRMGLKFSARAVVLTTGTFLG
                     GRIHVGLQNHPGGRAGDAPSNALAARLRELHPRVGRLKTGTPPRLDGRSIDWSVLAEQ
                     PGDQPRPVFSFMGSHAQHPRQVPCHITHTNEQTHEIIRSGFDRSPMYTGVIEGVGPRY
                     CPSVEDKVSRFAGKTSHQIFLEPEGLDTREIYPNGISTSLPYDVQERFVRSIRGLEQA
                     RIVRPGYAIEYDYFDPRDLSATLETKCIARLYFAGQINGTTGYEEAAAQGLLAGINAA
                     RSVFEKESWLPARHQSYIGVLVDDLVTRGTSEPYRMFTSRAEHRLLLREDN"
BASE COUNT          318 a          691 c          630 g          290 t
ORIGIN      
        1 ttgaccgcgc tgctcagcgg aaagctcgcg ctgctgtggc tgctgccggt gctcggctac
       61 ggcttcgcct gggtcggcca tttctacttc gagcacaacc ggccggccac gttcaagtac
      121 ccgttctaca gcctggcgtc cgatttcgtc atgtacaagg acatcctcac cggcaagatc
      181 cgcttctgac cgtggacggg cccgcgctga tcgccgggct ggaaaccggc tcgttgccgc
      241 cggcgcagtt cagccacgag aaccatgtgc gcgcggcgcg ccagttgctg ctggccgaac
      301 cgctgccgca ggccgcgcat cgcttcgccg aactgctgcg caactacgtg ggcggcatcg
      361 gcgcgcagga taaattccat ctcacgctga cgttggcctt catgcacctg atccaccagc
      421 gcatgcgcgc cggcgacagc tgggacgggt tccgcgcgcg ccacccggaa ctgttccgcg
      481 acgccgccgc gctgatcgcc cggcactaca gccgcgagcg catcgccgcc gggcggtcgg
      541 tgttcatcga gccggacggg ctgccgctgc cctgaaaatg cgcgagaatc cgcgcccctc
      601 atgaacaccc agcagtccta cgatgtcatc gtcatcggcg gcggccacgc cggcaccgag
      661 gccgcgctcg cggccgcgcg catgggtgcg cgcacgctgc tgctgacgca gaacatcgag
      721 acgctcgggc agatgtcgtg caacccggcc atcggcggca tcggcaaggg gcacctggtg
      781 cgcgagatcg acgccatggg cgggctgatg gcgcacgcgg ccgaccacgc cggcatccag
      841 ttccgcacgc tcaacgccag caagggcccg gcggtgcgcg ccacgcgggc gcaggccgat
      901 cgcgcgctgt acaagaaatt cgtccgcagc gcgctggaga accagccgca tctcgcgctg
      961 ttccagcagg aggtcgacga cctcgtcgtt gaaggcggct gcgtcaccgg cgtggttacg
     1021 cgcatgggcc tgaagttttc cgcgcgcgcg gtggtgctga ccaccggcac cttcctgggc
     1081 gggcgcatcc acgtggggct gcaaaaccat cccggcggcc gcgcgggcga cgcgccctcg
     1141 aatgcgctgg ccgcgcgcct gcgcgaactg catccgcgcg tgggccgcct gaagaccggt
     1201 acgccgccgc gcctggatgg ccgcagcatc gactggagcg tgctcgcgga gcagcccggc
     1261 gaccaaccgc gcccggtgtt ttccttcatg ggctcgcacg cgcagcatcc gcgccaggtg
     1321 ccgtgccaca tcacgcacac caacgagcag acgcacgaga tcatcaggag cggcttcgac
     1381 cgctcgccga tgtacaccgg cgtgatcgaa ggcgtcggcc cgcgctactg cccgagcgtg
     1441 gaagacaagg tgagccgctt cgccggcaag accagccacc agatcttcct cgagccggaa
     1501 ggcctcgaca cgcgcgagat ctaccccaac ggcatttcca ccagcctgcc ctacgacgtg
     1561 caggagcgct tcgtgcgcag cattcgcggg ctggagcagg ctcgcatcgt gcggcccggt
     1621 tacgccatcg agtacgacta cttcgatccg cgcgatctca gcgcgacgct ggaaaccaag
     1681 tgcatcgcgc ggctttattt cgccggccag atcaacggca ctaccgggta tgaagaggcc
     1741 gccgcgcagg gcctgctcgc cggcatcaac gctgcgcgtt cggtattcga aaaggaatcg
     1801 tggctgccgg cacggcacca gagctacatc ggcgtgctgg tcgacgacct ggtcacgcgc
     1861 ggcaccagcg agccgtaccg catgttcacc tcgcgcgccg agcatcgcct gctgctgcgc
     1921 gaggacaat
//
LOCUS       DASZZC010000439         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_411621,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000439 DASZZC010000000
VERSION     DASZZC010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411621"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2205)
                     /locus_tag="VHE37_09315"
     CDS             complement(<1..2205)
                     /locus_tag="VHE37_09315"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HVT35772.1"
                     /translation="MKRAAWCLALLMLQGCSTGAPDPLPADDGDGGSGSATGATTGAG
                     TGSTTGTTTGTATGSSTGGTTGSPPPADTAPVAHAGAAQVVDENLAVVLNGSASSDAD
                     GDPLSYAWTQTGGPAVTLSGADGAAPQFTSPEVADDSTLDFQLTVSDGRGGSSSDLTH
                     VTVKHYAANLSQYMVPLTGTMPPGFVNPGPFAPFGLVHVGPDSEGPLNYGGYSYQNTL
                     ISGFSHVHMSAGVYKGGEIPFMPLTGNVTPGDLSSTGWPSEVPAYASPFTHVLESASA
                     GYYSVTLLRYGVQAELTATPRAAMHRYTYQLPGQTPRLLIDVSRDLRGHHTASATMHG
                     DGTLTGSVTTDDVGGYTVYFAAKFSAPYTAQTLDGTALAADQAVGGDTLGVLLDFPSL
                     GGPLVSKIGLSYVDEAGALRNLDAEIPGWDFDAVHAQAVSTWDHQLAKLAVTGGSAQQ
                     LQSFYTALARALEFPNLFSDVDGRYAGAEDTSVIHSDTRAHYSEYSLWDSYRGQNALI
                     AELEPGIYGDMVNSLLDFARQHGRLPRWQQAQRDAGHMSGDPAIPFIGEAWCRGASEA
                     ATRDELYAGMVALTQARAAEIASGYKPVDPPANVFAQLTGGPGGTGTTLEYGIADFSL
                     ALMAHASAHDNDAAAFTQRSLNYRNLIDPDGSGLVRPRLADGSWITPFITTEPYGFQE
                     GTSWQYSWLAMHDIAGLVERMGGAAAVTQKLDTLFSFPANAVPLAVPLLQDQI"
BASE COUNT          325 a          741 c          807 g          367 t
ORIGIN      
        1 gatctggtcc tgcagaagcg gcaccgccag cggcaccgcg ttggccggga aggagaagag
       61 ggtgtcgagc ttctgcgtca ccgccgccgc gccgcccatg cgctcgacca gcccggcgat
      121 gtcgtgcatc gccagccacg agtactgcca cgaggtgcct tcctggaatc cgtacggctc
      181 ggtggtgatg aacggcgtga tccagctgcc gtcggccagg cgcgggcgga ccaggccgga
      241 gccgtcgggg tcgatcaggt tgcggtagtt gagcgagcgc tgcgtgaacg cggccgcgtc
      301 gttgtcgtgc gcggaggcgt gcgccatcag cgccagcgaa aaatcggcaa tgccgtattc
      361 cagcgtggtg ccggtgccac ccggcccgcc ggtcagctgc gcaaacacat tcgccggcgg
      421 atcgaccggc ttgtagccgg atgcgatttc cgcggcgcgc gcctgcgtga gcgcgaccat
      481 gcccgcgtac agctcgtcgc gcgttgcggc ttcgctggcg ccgcggcacc aggcttcgcc
      541 gatgaaggga atcgccggat cgcccgacat gtggccggcg tcgcgctgcg cctgctgcca
      601 gcgcggcagg cggccgtgct ggcgcgcgaa gtcgagcagc gaattcacca tgtcgccgta
      661 aatgcccggc tccagctcgg cgatcagcgc gttctgcccg cggtagctgt cccacagcga
      721 gtattcgctg tagtgcgcgc gcgtgtcgct gtggatcacg ctggtgtctt ccgcaccggc
      781 gtagcggccg tcgacgtcgg aaaacagatt tggaaattcc agcgcgcgcg ccagcgcggt
      841 gtagaacgac tgcagctgct gcgcgctgcc gccggtcacc gcgagcttcg ccagctggtg
      901 atcccacgtg ctcaccgcct gcgcatgcac cgcgtcgaaa tcccagccgg ggatttccgc
      961 atcgaggttg cgcagcgcgc cggcttcatc cacgtatgaa aggccgatct tgctcaccag
     1021 cggcccgccg agcgacggaa aatccagcag cacgccgagc gtatcgccgc ccacggcctg
     1081 gtccgcggcg agcgcggtgc cgtcgagtgt ctgcgcggtg tacggcgcgg aaaatttcgc
     1141 ggcaaagtaa accgtgtagc cgccgacgtc gtcggtggtg acgctgccgg tgagcgtgcc
     1201 gtcgccgtgc atcgttgcgc tggcggtgtg gtgtccgcgc aggtcgcgcg agacgtcgat
     1261 cagcaggcgc ggcgtctgcc ccggcagctg gtaggtgtag cggtgcatcg cagcgcgcgg
     1321 cgtggcggtg agctcggcct gcacgccgta gcgcaacagt gtcaccgagt agtagccggc
     1381 gctggcgctc tccagcacgt gcgtaaaggg tgatgcatac gccggcactt cactgggcca
     1441 gccggtggag gagagatcgc ctggcgtgac attgccggtg agcggcatga acggaatctc
     1501 gccgcccttg tacacgcctg cgctcatatg cacgtgcgaa aaacccgaaa tcagcgtgtt
     1561 ctggtacgag tagccgccgt agttgagcgg gccttcgctg tccggcccca cgtgcaccag
     1621 gccaaacggc gcgaagggac ctgggttgac gaagcccggc ggcatcgtgc cggtgagcgg
     1681 caccatgtat tgcgacaggt tggctgcgta atgcttcacc gtgacgtgcg tgaggtcact
     1741 cgaactgccg ccgcggccgt cgctgacggt gagctggaaa tcgagcgtgc tgtcatccgc
     1801 gacttcgggc gaggtgaact gcggcgcggc gccatcggcg ccggacagcg tcaccgccgg
     1861 gccgccggtc tgcgtccagg catagctgag cgggtcgccg tcggcgtcgc tgctggcgct
     1921 gccgttcagt acgaccgcga ggttttcatc caccacctgc gcggcgccgg catgcgcgac
     1981 cggcgcggtg tccgcgggag gagggctgcc ggtggtgcca cccgtgctcg aaccggtggc
     2041 ggtgccggtg gtcgtgcccg tggtgctgcc ggtgcccgcg cctgtcgtcg cgccggtggc
     2101 ggagccgctg ccgccatcac cgtcgtcagc cggcagcgga tccggcgcgc cggtgctgca
     2161 gccctgcagc atcagcaacg cgaggcacca cgcggcgcgc ttcacggcag cgtgatcggc
     2221 gcgctggttt cgttgtgatc
//
LOCUS       DASZZC010000440         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1191702,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000440 DASZZC010000000
VERSION     DASZZC010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191702"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VHE37_09320"
     CDS             complement(<1..1126)
                     /locus_tag="VHE37_09320"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HVT35773.1"
                     /translation="MSYQLARIRADFPILAESVHGKRLAYLDNAATTQKPESVLRAMD
                     HYYRHQNANVHRAVHELASRATAAYEGARERIARYLHATRDEIVFTRGTTEAINLVAR
                     SFLAPRLQQGDEVLLTTMEHHSNIVPWQLAGAKTVAAPVNDAGELIFDQWLARFSSRT
                     KLVAVAHCSNSLGTVNPVAEMIREAHARGVPVLVDGAQAIAHTAVDVKDLDVDFYACS
                     AHKAYGPTGFGALYAKREHLEAMPPLQGGGDMIRTVAFDGSTWNDVPYKFEAGTPDMA
                     GAIGFAAALDYIDSIGLAAIGTHEHALLEYATQQIARVPGLKPVGTARNKGGILSFVM
                     DGAHPHDIGAILDQAGVAIRTGHHCTMPLMQRFNIPATARA"
     gene            complement(1123..2223)
                     /gene="sufD"
                     /locus_tag="VHE37_09325"
     CDS             complement(1123..2223)
                     /gene="sufD"
                     /locus_tag="VHE37_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HVT35774.1"
                     /translation="MSAALRQYIDAFEQFSAALPREEKSAREKELAAFLAQGFPHRKI
                     EEWKYTDLSALADKTYAQAPAVAGGLAPVRGSSSVAHLNAAFAQPGLQLRVAAGAQAE
                     DEYNTRMDGARMQHLHHRIALAADAQATVILHHAGSGEYLTTQVLDIDLADGAQLSLY
                     RVQDEAGGSHHLAHTSAQLGRDASITVVTVDLGAGLARHDLDIALTAPGASAQVHGLY
                     VPARGAHVDNHTRIDHRAPHGSSREFFRGIVGERCRAIFNGKVIVQEGAVKTDSEQRV
                     ANLLLSPRAEVNAKPELEIYNDDVKCAHGATFGQLDESAIYYLRARGIGREQARALLT
                     HAFAQEVLQHLAHEPTRTLVTRRLDALLGAAA"
     gene            complement(2220..2966)
                     /gene="sufC"
                     /locus_tag="VHE37_09330"
     CDS             complement(2220..2966)
                     /gene="sufC"
                     /locus_tag="VHE37_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HVT35775.1"
                     /translation="MLEIKNLHAKVDAREILRGIDLTIRAGEVHAIMGPNGSGKSTLS
                     HLLAGREGYTVTQGSVSFEGKDLLALEPEARACEGVFMGFQYPVEIPGVSNMVFLKAA
                     LNARRKYRGEKEIDAPDFLAWVKDRVAKMDMNTKMLQRGVNEGFSGGEKKRNEILQML
                     ILEPRLMILDETDSGLDIDALKVVANGINLMRSPQRATLLVTHYQRLLDYVKPDFVHV
                     LAGGRIVRSGGPELALELEQKGYGWIEQAA"
     gene            complement(3005..>3525)
                     /locus_tag="VHE37_09335"
     CDS             complement(3005..>3525)
                     /locus_tag="VHE37_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HVT35776.1"
                     /translation="AITWKYPSCILRGDSSIGEFYSVAVTHHRQQADTGTKMIHIGRN
                     TRSTIVSKGISAGHGQQSYRGLVRVFPSAHGARNHTQCDSLLIGAHCGAHTFPYTEVR
                     NPTAQLEHEATTSKIGEDQLYYARSRGIAEEDAVSMIVNGFCKDVFKELPMEFAVEAQ
                     KLLSVTLEGAVG"
BASE COUNT          498 a         1160 c         1220 g          647 t
ORIGIN      
        1 aggcgcgcgc ggtggcggga atgttgaagc gctgcatcaa cggcatggtg caatggtggc
       61 cggtgcggat ggcgacgccg gcctggtcga ggatggcgcc gatgtcgtgc gggtgcgcgc
      121 cgtccatcac gaacgacagg atgccgccct tgttgcgcgc ggtgccgact ggcttgagtc
      181 ctggcacgcg cgcgatctgc tgcgtggcgt attcgagcag cgcgtgctca tgcgtgccga
      241 tggccgccag cccgatcgag tcgatgtaat cgagcgccgc cgcgaagccg atggccccgg
      301 ccatgtccgg cgtgccggcc tcgaacttgt acggcacgtc gttccaggtg ctgccgtcga
      361 aggcgacggt cctgatcatg tcgccgccgc cctgcagcgg cggcatggct tcgagatgct
      421 cacgcttcgc gtagagcgcg ccgaaaccgg tcgggccgta ggccttgtgc gccgagcatg
      481 cgtagaagtc cacgtcgaga tccttgacgt cgacggccgt gtgcgcgatc gcctgcgcgc
      541 catccaccag caccggcacg ccgcgcgcgt gggcttcgcg gatcatctcg gcaaccggat
      601 tcaccgtgcc cagcgagttg gagcagtgcg ccaccgccac cagtttcgtg cgcgatgaaa
      661 agcgcgcgag ccactggtcg aagatcagct cgccggcatc gttcaccggt gcggccaccg
      721 tcttcgcccc ggccagctgc cagggcacga tgttggagtg gtgctccatc gtcgtcagca
      781 gcacttcatc gccctgctgg aggcgcggcg ccaggaacga gcgcgccacc aggttgatcg
      841 cttccgtggt gccgcgagtg aacacgatct cgtcgcgcgt ggcgtgcagg tagcgcgcga
      901 tgcgttcgcg cgcgccctcg tacgcagccg tggcgcgcga ggccagctcg tgcaccgcgc
      961 gatgcacgtt ggcgttctgg tgccggtagt aatggtccat cgcgcgcagc acgctctcgg
     1021 gcttctgcgt ggtggcggcg ttgtccaggt aggccagccg cttgccgtgc acgctttccg
     1081 cgaggatggg gaaatccgcc cggatgcggg cgagctggta gctcatgccg ccgctcccag
     1141 cagcgcgtcg agacgccgcg tgaccagcgt acgcgtcggc tcgtgtgcca ggtgctgcag
     1201 cacttcctgc gcaaacgcgt gcgtcagcag cgcgcgcgcc tgctcgcgcc cgatgccgcg
     1261 cgcgcgcagg taatagatgg cgctctcatc cagctggccg aaggtggcgc cgtgcgcaca
     1321 tttcacgtcg tcgttgtaga tctccagttc gggcttggcg ttgacctcgg ccctgggcga
     1381 cagcagcagg ttggccacgc gctgttccga atcggtcttc accgcgcctt cctgcacgat
     1441 gaccttgccg ttgaagatgg cacggcaacg ttcgccgacg atgccgcgga agaactcgcg
     1501 gctcgaccca tgcggcgcgc ggtgatcgat gcgcgtgtgg ttgtccacgt gcgcgccgcg
     1561 cgcaggcacg tacagcccgt gcacctgcgc gctggcgccg ggcgcggtga gcgcgatgtc
     1621 caggtcgtgc cgcgcgaggc cggcgccgag atctaccgtc accaccgtga tgctcgcgtc
     1681 gcgcccgagc tgcgccgagg tatgcgccag gtgatggctg ccgcccgcct cgtcctgcac
     1741 tcggtagagc gagagttgcg caccatcggc caggtcgatg tccagcacct gcgtggtgag
     1801 gtattcgccg gagccggcgt gatgcaggat caccgtcgcc tgcgcatcgg ctgccagcgc
     1861 gatgcggtga tgcaggtgct gcatgcgcgc gccgtccatg cgggtgttgt actcgtcctc
     1921 cgcctgcgcc cccgcagcga cgcgcagttg caggccgggc tgtgcgaacg cagcgtttag
     1981 gtgcgccacg ctgctgctgc cgcgcaccgg tgcaagaccg ccggcgaccg cgggcgcctg
     2041 cgcgtaggtc ttgtcggcga gggcggaaag gtcggtgtac ttccactctt cgatcttgcg
     2101 atgcgggaag ccttgcgcga ggaacgcggc gagttccttc tcgcgcgccg atttttcctc
     2161 gcgcggcagc gccgccgaga actgctcgaa ggcgtcgatg tactggcgca gcgccgcgct
     2221 catgccgcct gctcgatcca gccgtagccc ttctgctcga gctccagcgc cagttccggg
     2281 ccgccgctcc tgacgatgcg tccgccggcg agcacgtgca cgaaatccgg tttcacgtaa
     2341 tcgagcaggc gctggtaatg cgtcaccagc agcgtggcgc gctgcggcga gcgcatcagg
     2401 ttgatgccgt tggccaccac cttcagcgcg tcgatatcca ggccggaatc ggtctcgtcg
     2461 aggatcatca gccgcggctc gaggatcagc atctgcaaaa tctcgttgcg cttcttctcg
     2521 ccgccggaga agccctcgtt gacgccgcgc tggagcatct tggtgttcat gtccattttc
     2581 gccacgcggt ccttgaccca ggcgaggaag tcgggcgcgt cgatctcctt ctcgccgcgg
     2641 tacttgcgcc gcgcgttgag cgccgccttg aggaacacca tgttcgatac gccgggaatt
     2701 tccaccgggt actggaagcc catgaacacg ccttcgcagg cgcgcgcctc gggttcaagc
     2761 gcgagcaggt ccttgccctc gaaactcacg ctgccctggg tcacggtgta gccctcgcgc
     2821 ccggccagca ggtgcgacag cgtggacttg cccgagccgt tcggacccat gatggcgtgc
     2881 acttcgccgg cacggatggt gaggtcgatg ccgcgcagga tttcccgggc gtctactttc
     2941 gcgtggagat ttttgatttc gagcatggtg gctggggccg cacggccctg gctggtaata
     3001 gtggttaacc gacggcgcct tcgagcgtga ccgagagcag cttctgcgcc tcgacggcga
     3061 actccatcgg cagttccttg aacacgtcct tgcagaagcc gttgacgatc atcgacaccg
     3121 cgtcttcctc ggcgatgccg cgcgagcgcg cgtagtacag ctggtcctcg ccgatcttgg
     3181 aggtggtggc ttcgtgttcg agctgcgccg tggggttgcg cacctcggtg taggggaagg
     3241 tgtgcgcgcc gcaatgcgcg ccgatcagca gagaatcgca ctgcgtgtgg ttgcgcgcgc
     3301 catgcgcgga cgggaacacg cgcaccaggc cgcggtagct ctgctgcccg tggccggccg
     3361 agatgccctt gctgacgatg gtcgagcgcg tgttgcgtcc gatgtggatc atcttggtgc
     3421 cggtgtcggc ctgctggcgg tggtgcgtca ccgccacgct gtagaactcg ccgatggagc
     3481 tgtcgccgcg caggatgcag ctcgggtatt tccaggtgat ggccg
//
LOCUS       DASZZC010000441         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_614377,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000441 DASZZC010000000
VERSION     DASZZC010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614377"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..240)
                     /locus_tag="VHE37_09340"
     tRNA            complement(165..240)
                     /locus_tag="VHE37_09340"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Asn,seq:gtt)
     gene            complement(321..1505)
                     /locus_tag="VHE37_09345"
     CDS             complement(321..1505)
                     /locus_tag="VHE37_09345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVT35777.1"
                     /translation="MELTLAQRVGRIKPSPTLAVTAKAGELKAQGKDVLSLGAGEPDF
                     DTPAHIKAAAQKAMDSGFTKYTPVGGTPGLKKAIIDKMKRDNGLDYKPNQVLVSCGGK
                     QSCYNLCQALLNATDEVIVPAPYWVSYPDMVLLADATPVFIYTSAATRFKITPAQLEA
                     AITPRTRMIWLNSPSNPSGMAYTRAELAALGEVLRKHPNIVIASDDMYEKILWTAEPF
                     CTLLNVCPDLYDRTVIIHAVSKTYSMTGWRIGWACGPAKLITAMADIQSQSTSNPNSI
                     AQVAAEAALNGDQACVSEMVKAFKARHDALVADLNTVPGVQCLSGDGTFYVFPNVEGL
                     MAKLGCKTDLELADLLLQKHLVALVPGSAFGAPGHVRLSFATSDKVLKGSVERMRAAA
                     KG"
     gene            complement(1721..3064)
                     /gene="astB"
                     /locus_tag="VHE37_09350"
     CDS             complement(1721..3064)
                     /gene="astB"
                     /locus_tag="VHE37_09350"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HVT35778.1"
                     /translation="MSGAAAEANFDGLVGPTHNYAGLSLGNVASLKHAQQASNPREAA
                     LQGLAKMHALAELGLHQGVLPPHERPHIPTLKKLGFGGSEAAVLARAAAEAPALLAAA
                     SSASAMWVANAATVSPSADTADRRVHFTPANLCSHLHRAIEPRMTARILKATFRAREH
                     FAHHLPLPSTPSYGDEGAANHTRLCSEYGEAGLELFAYGRGYSHEPAPQKFPARQTRH
                     ACAALVRLHGLQPEATHLALQSPEVIDQGVFHNDVIAVGNREVLLYHERAYADAAALR
                     AFVCTHLPGARRPVFIEVRDDEVSVEQAVSSYLFNSQLVCPPGGGMLLVCAAECREQP
                     QVWRRIEQIVADDANPITEARLFDLHQSMHNGGGPACLRLRVVLTGQERAAVNPRSWI
                     TPLRYRELCDWVQRHYRDRLEPRDLADPKLLYESRRALDELTQLLGLGMIYDFQR"
     gene            complement(3061..>3517)
                     /locus_tag="VHE37_09355"
     CDS             complement(3061..>3517)
                     /locus_tag="VHE37_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HVT35779.1"
                     /translation="LDVAAVSIPAQVDMPQLVVREGGGQVAVAEQQQWIAPLADEIRN
                     ALSADLTQRLHAVDVHRAARSADDPVWRVQLDVQRFDSLLGQRAHMEAVWSLRAPGKD
                     AAALSCPASADVPVSAGYAALVEGHQQALRQIAAQIAGAIEAGSAARCP"
BASE COUNT          541 a         1159 c         1215 g          602 t
ORIGIN      
        1 agactcgaca aatttcgcag ggaagcgaaa tttgagcgcg cggatgcgcg cccgtgaggg
       61 cgcgggccag gagggcccgc gcaacttggg acgaagtccc tggtgagagt ccgtgacaat
      121 gaaaaagccc cgggatgacg cccggggctt tcaaaaaaaa tagttggctc cgcgacctgg
      181 actcgaacca gggacccagt gattaacagt cacttgctct accgactgag ctatcgcgga
      241 tcggtggggc gcgcattgta gtgagcaggt cgcgcgggcg caattgtcgc gcgcccgccc
      301 cccgcgtcga tccgctttct tcagcccttg gccgccgcgc gcatgcgctc gacgctgccc
      361 ttcagcacct tgtcgctggt ggcgaacgac agccgcacgt ggccgggcgc gccgaaggcc
      421 gaacccggca ccagcgccac caggtgcttc tgcagcagca ggtcggccag ttccaggtcg
      481 gtcttgcagc cgagttttgc cattagccct tccacgttgg ggaagacata gaaggtgccg
      541 tcgcccgaca ggcactgcac gccgggcacc gtgttgagat cggccaccag cgcgtcgtgg
      601 cgcgccttga acgctttgac catctcactc acgcaagcct ggtcgccgtt gagcgcggcc
      661 tctgcggcca cctgcgcgat ggagttcggg ttcgacgtcg actgcgactg gatgtcggcc
      721 atcgcggtga tgagtttcgc cggcccgcag gcccagccga tgcgccagcc ggtcatcgaa
      781 taggtcttcg acaccgcgtg gatgatgacc gtgcggtcgt aaagatccgg gcagacattg
      841 agcagcgtgc agaacggctc ggcggtccac aggatcttct cgtacatgtc gtccgacgcg
      901 atgacgatgt tcgggtgctt gcgcagcacc tcgcccagcg cggcgagttc cgcgcgcgtg
      961 taggccatgc ccgacgggtt gctcgggctg ttgagccaga tcatgcgcgt gcgcggcgtg
     1021 atcgccgctt ccagctgagc cggtgtgatc ttgaagcgcg tcgccgcgct ggtgtagatg
     1081 aacaccggcg tggcgtcggc cagcagcacc atgtccgggt agctgaccca gtacggcgcc
     1141 ggcacgatca cttcgtcggt cgcgttgagc agcgcctggc acaggttgta gcaggactgc
     1201 ttgccgccgc acgacaccag cacctggttg ggcttgtagt cgagcccgtt gtcgcgcttc
     1261 atcttgtcga tgatggcttt cttgaggccc ggcgtgccgc ccaccggcgt gtacttggtg
     1321 aagccgctgt ccatcgcctt ctgcgcggcg gccttgatgt gcgcgggcgt gtcgaagtcc
     1381 ggctcgccgg cgcccagcga gagcacgtcc ttgccctgag ctttcagttc accggccttg
     1441 gcggtgacgg cgagggtggg cgagggcttg atgcggccga cgcgctgggc gagtgtcagt
     1501 tccacggtgc gtttccttct gcggtccaaa aaagcgcgcg cgatgatact tgagtgcgcg
     1561 cagcgccgca tttgcgccct ggcaggcccg cggaagtggt caccgcacct tcatttttga
     1621 tgttattaat tatataatat atttaaataa aataatattt tataacatca aacagggcag
     1681 tgcggagagg cgattttgcg cccgttgcgc tgatgaagga tcagcgctgg aagtcgtaga
     1741 tcatgcccag cccgagcagc tgggtgagtt cgtcgagcgc acggcgcgat tcgtagagca
     1801 gcttcggatc ggccaggtca cgcggctcca ggcggtcgcg gtaatggcgc tgcacccagt
     1861 cgcacagctc gcggtagcgc agcggcgtga tccacgagcg cggattcacc gctgcgcgct
     1921 cctgcccggt cagcaccacg cgcaggcgca ggcaggcggg gccgccgccg ttgtgcatcg
     1981 actggtgcag gtcgaacaag cgcgcttcgg ttatcgggtt ggcatcgtcg gcaacgatct
     2041 gctcgatgcg ccgccacacc tgcggctgct cacggcactc ggccgcgcat accagcagca
     2101 tgccgccgcc gggcggacag accagctgcg agttgaacag gtaggacgac accgcctgct
     2161 caacgctcac ctcgtcgtcg cgcacctcga tgaacaccgg ccgtcgcgcg ccgggcaggt
     2221 gcgtgcatac gaacgcgcgc agcgcggcgg cgtcggcgta ggcgcgctcg tggtacagca
     2281 gcacttcgcg gttgcccacc gcgatcacgt cgttgtggaa cacgccctgg tcgatcacct
     2341 ccggtgattg cagcgccagg tgcgtggcct cgggctgcag cccgtgcagg cgcacgagcg
     2401 cggcgcaggc gtggcgcgtt tgccgcgcgg gaaatttctg cggcgccggt tcatgcgagt
     2461 agccgcggcc gtaggcgaac agctccaggc cggcctcgcc gtattcgctg cacaggcgcg
     2521 tgtggttggc ggcgccttcg tcaccgtacg aaggcgtcga aggcagcggc aggtgatgcg
     2581 cgaaatgctc gcgcgcgcgg aatgtcgcct tgaggatgcg cgcggtcatg cgcggttcga
     2641 tggcgcggtg caggtggctg cacaggttgg ccggcgtgaa gtgcacgcgg cggtcggcgg
     2701 tgtcggccga cggtgacacc gtcgccgcgt tggccaccca catcgccgag gcggaactcg
     2761 ccgcggccag cagcgcgggt gcttcggcag ccgcgcgggc cagcaccgcg gcctcgctgc
     2821 cgccgaagcc gagcttcttc agcgtgggga tatgcggccg ctcgtgcggc ggcagcacgc
     2881 cctggtgcag gcccagctcc gccagcgcat gcatcttggc caggccctgc agcgcggctt
     2941 cgcgcggatt cgacgcctgc tgcgcatgct tcagcgaagc gacattgccc agcgagagac
     3001 cggcgtagtt gtgcgtcggc ccgaccaggc cgtcgaagtt ggcttcggcg gcggcgccgc
     3061 tcatgggcaa cgcgcggcgc tgcccgcttc gatggcgccg gcgatctgtg cggcgatctg
     3121 gcgcagcgcc tgctggtggc cttcgaccag cgcggcgtag ccggcactca ccggtacatc
     3181 ggcgctggcc gggcacgaca gcgccgccgc atccttgccc ggcgcgcgca gcgaccacac
     3241 cgcttccatg tgcgcgcgct ggcccagcag cgagtcgaaa cgctgcacgt ccagctgcac
     3301 gcgccacacc gggtcgtccg cgctgcgcgc ggcgcggtgc acgtcgaccg cgtgcaggcg
     3361 ttgcgtcagg tcggcggaca gcgcgttgcg gatctcgtcg gccagcggcg caatccactg
     3421 ctgctgctcg gccaccgcca cctggccgcc gccctcgcgc accaccagct gcggcatgtc
     3481 gacctgcgcg ggaatgctca ccgcggccac gtcgagc
//
LOCUS       DASZZC010000442         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1062958,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000442 DASZZC010000000
VERSION     DASZZC010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062958"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1609
                     /locus_tag="VHE37_09360"
     CDS             203..1609
                     /locus_tag="VHE37_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT35780.1"
                     /translation="MKRGKGESSKTTRGVDRNRRRILAGLAAAPAAAALSSCGSDSAA
                     TSSVIASLPDPAMSGIDHIVVVMMENRSFDHYLGWLPGADGKQAGLSYPDVNGALQST
                     YPLAPEFQSCGKQDPDHSHEGGVKQYNNGACDGFLQTSPTDTFPIGYFTQADLSFTGQ
                     AAPAWTVYDRYFCSILAETYPNRFYMHGAQTPQIHNDTTAALTNPATIPTIWDRLRAA
                     GRTGTYYFSDVPFVGLWGSTYLSIAQPYEKFLVDCALGNLPDVSYIDPAFEDEGSGTS
                     RDDHPFADIRDGQAFLSQIYNAVTSSPQWEKTALFITYDEWGGFFDHVPPPDAGFIPP
                     ADLAACQAENVVPFSRMGFRVPTMLISPRARRGHVSHEQYDHCSILKMIEWRFGLPPL
                     TLRDAQANNIANSFDFVSPPDLTLPTFNAVPLGPFGLPCALSSLPLGSNAAAIREKHF
                     AEVNGLKALARQYGFAVR"
     gene            complement(1611..>2292)
                     /locus_tag="VHE37_09365"
     CDS             complement(1611..>2292)
                     /locus_tag="VHE37_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35781.1"
                     /translation="AAANRMTGVSIERRGAFSGDVNPEDVSLAIRWVRKNIARYGGDP
                     NHIVVAGESAGGNLALSVAVASSYRRPEPWAQMVYDARVRPLAVQPIMPYLQVSNPAR
                     HSLNAASGFMALDVAHDIAAAYLGRRNTQASAETLMADPIRVLEECGAPERPLPQVWS
                     CVGTADLCCEDVHRLEMACKKHDVPAQIDYYENEIHAFHAFRWRPAAQRFWRQNFEFL
                     EELAAPAL"
BASE COUNT          396 a          809 c          710 g          377 t
ORIGIN      
        1 tcggcccggg cgtctgatcc attggcaaaa acagctgcac cgcactgccc tggatgatgt
       61 tattaattat taatcaatat taaaaacgat caatatttaa taacacagcc ggaggcggtg
      121 cggtaagaca aaaaatcgct gctcaacact gcgtaatgcg caccgcggct atgatcttgc
      181 gcagcgtgca cgggagatcg ccatgaagcg aggcaaggga gaatcatcga agaccacgcg
      241 cggcgttgac cgcaaccgcc ggcgcatcct cgccggcctt gccgcagcgc cggccgcggc
      301 ggcgctgtcc agctgcggca gcgattccgc cgccacctct tccgtcatcg cgtccctgcc
      361 cgatccggcg atgtccggca tcgatcacat cgtggtggtg atgatggaga accgctcgtt
      421 cgatcattac ctcggctggc tgccgggcgc cgacggcaaa caggcaggcc tgtcctaccc
      481 cgacgtcaac ggcgcgctgc agagcaccta tccgctggcg ccggaattcc agagctgcgg
      541 caagcaggac ccggatcact cgcatgaagg cggcgtgaag cagtacaaca acggcgcgtg
      601 cgacggtttc ctgcagacca gcccgaccga caccttcccc atcggctact tcacccaggc
      661 cgatctctcc ttcaccggcc aggccgcgcc ggcgtggacc gtgtacgacc gctacttctg
      721 ctcgatcctg gccgagacct atccgaaccg tttctacatg cacggcgcgc agacgccgca
      781 gatccacaac gacaccactg cggccctgac caacccggcg accatcccga ccatctggga
      841 ccgcctgcgc gcggccggcc gcaccggcac ctattacttc agcgacgtgc cgttcgtggg
      901 cctgtggggc agcacctacc tgtccattgc gcagccgtat gaaaaattcc tggtcgactg
      961 cgcgctgggc aacctgccgg atgtgagcta catcgacccg gcgttcgagg acgagggcag
     1021 cggcacctcg cgcgacgacc atccgttcgc cgacatccgc gacggccagg cctttctctc
     1081 gcagatctac aacgcggtga ccagcagccc gcagtgggag aagaccgcgc tgttcatcac
     1141 ctatgacgag tggggcggct tcttcgacca cgtgccgccg ccggacgccg gcttcattcc
     1201 gccggccgac ctggcggcct gccaggcgga gaacgtggtg ccgttctcgc gcatgggctt
     1261 ccgcgtgccg accatgctga tttcgccgcg tgcgcggcgc ggccacgtgt cgcacgagca
     1321 gtacgaccat tgctccatcc tgaagatgat cgaatggcgc ttcggcctgc cgccgctgac
     1381 gctgcgcgat gcgcaggcca acaacatcgc caactcgttc gacttcgtca gcccgcccga
     1441 cctcacgctg cccacgttca acgcggtgcc gctggggccg ttcggcctgc cgtgcgcgct
     1501 gtcgagcctg ccgctgggat cgaacgcagc ggccatccgc gaaaagcact tcgccgaagt
     1561 gaacggactg aaggcgctgg cgcgccagta cggcttcgcg gtgcgctagc tcacagcgcc
     1621 ggcgccgcca gttcttccag gaattcgaag ttctgccgcc agaagcgctg cgccgccggg
     1681 cgccagcgga aggcgtggaa ggcgtggatc tcgttctcgt agtaatcgat ctgcgccggc
     1741 acgtcgtgct tcttgcacgc catctccagc cggtgcacat cctcgcagca caggtcggcg
     1801 gtgccgacgc aggaccacac ctgcggcagc ggccgctcgg gcgcgccgca ttcctcgagc
     1861 acgcggatcg ggtcggccat cagcgtctcg gcgctggcct gcgtgttgcg ccggccgaga
     1921 taggctgcgg cgatgtcatg cgccacgtcc agcgccatga agccgctggc cgcgttgagc
     1981 gaatggcgcg ccgggttgct cacctgcagg tacggcatga tcggctgcac cgccagcggc
     2041 cgcaccctgg cgtcgtacac catctgcgcc cacggctccg gccggcgata gctcgacgcc
     2101 acagcgaccg acagcgccag attgccgcca gcggattcgc cggccaccac gatgtggttc
     2161 ggatcgccgc cgtagcgggc gatattcttc cgcacccatc ggatcgccag ggaaacgtcc
     2221 tcggggttga cgtcgccgga gaaggcgccg cggcgttcga tgctcacgcc cgtcatccgg
     2281 ttggcggcgg cc
//
LOCUS       DASZZC010000443         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1002991,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000443 DASZZC010000000
VERSION     DASZZC010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002991"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="lgt"
                     /locus_tag="VHE37_09370"
     CDS             <1..895
                     /gene="lgt"
                     /locus_tag="VHE37_09370"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HVT35782.1"
                     /translation="HPNFDPVAFRLGPLAVHWYGLMYLIGFWGCWWIGTRRARQPHIQ
                     WTEQQLSDLVFYVIMGVILGGRVGYTLFYNLPNFLHDTPLDYLRWLAKIFYDWHGFAT
                     TVPVIFRLWEGGMSFHGGLLGVVIAYLLLARKLKMHPFDIGDFAAPLIPFGLFTGRIG
                     NFINGELWGAPTTLPWGMVFQHAGPEPRHPTMLYEAGLEGLAMLGILWWFGRQPRPRM
                     AVAALFILLYALFRTMVEFVRLPDAQIGYLFGTGWITMGMTLCVPMWIAGAIIMFLAY
                     RRPVYTPAAQRVLDAQQKGAA"
     gene            898..1692
                     /locus_tag="VHE37_09375"
     CDS             898..1692
                     /locus_tag="VHE37_09375"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HVT35783.1"
                     /translation="MRQYLDLMRHVLETGTAKTDRTGTGTLSVFGHQMRFDLAQGFPL
                     VTTKKLHLKSIVYELLWFLRGETNTRYLKEHGVSIWDEWADDNGDLGPVYGYQWRSWP
                     TRDGRHIDQIQQVLDQLKNSPDSRRIIVSAWNVGELEQMALMPCHALFQFYVADGRLS
                     CQLYQRSADIFLGVPFNIASYALLTLMVAQVSGLKPGEFIWTGGDCHLYSNHLEQART
                     QLARAPMPLPRMKLNPEVKDLFAFRYEDFALEGYESHPHIKAPVAV"
     gene            1890..2969
                     /locus_tag="VHE37_09380"
     CDS             1890..2969
                     /locus_tag="VHE37_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35784.1"
                     /translation="MKFNTRVFSAAAIVACAAALSACGGGGSSSSSAAVSTAPGQIID
                     TSKCLATAGPFDAAQNAIVSGLGGLTSSLPQGASVASSLTNAASHLLDTPDAVVAALQ
                     NLAANHDPAAFAAELTSAGDGLRCGLAYTDSALLDLTQQLQNQSASVPALPSLLSQVE
                     AIETMLGQGLTGQTDLTNLTNALGALQTQLERVNSQLPAGFKIPNISLPMSMIGNIAT
                     SASVILQSVAHLDAGSAQGAITGLALQLAGLVNGYSALGLPGAVVQPIYSLLLSGNTQ
                     FVSNIVPYIGPLFTAITRVLQSQSNPLGTLQQIVMGGLSGVPASGAFASLLNLLNGGG
                     TSSTGAQIPLLSDLLALLIPGGVPL"
BASE COUNT          510 a         1110 c         1025 g          575 t
ORIGIN      
        1 acatccgaat ttcgaccccg tcgctttccg cctggggccg ctggcggtgc attggtacgg
       61 cctcatgtac ctgatcggct tctggggctg ctggtggatc ggcacgcgcc gcgcgcgcca
      121 gccgcacatc cagtggaccg agcagcagct gtcggacctg gtgttctacg tgatcatggg
      181 cgtgatcctc ggcggccgcg tgggctacac gctgttctac aacctgccca acttcctgca
      241 cgacacgccg ctggattacc tgcgctggct ggcgaaaatt ttctacgact ggcacggctt
      301 cgctaccacg gtgccggtga tcttccgcct gtgggagggc ggcatgagct tccacggcgg
      361 gttgctcggc gtggtcatcg cctacctgct gctggcgcgc aagctgaaga tgcatccgtt
      421 cgacatcggc gacttcgccg cgccgctgat cccgttcggt ttgttcaccg ggcgcatcgg
      481 caatttcatc aacggcgagc tgtggggcgc gccgaccacg ctgccctggg gcatggtgtt
      541 ccagcacgcc gggccggagc cgcgccatcc caccatgctg tacgaggccg gactggaagg
      601 cctggccatg ctcggcattc tttggtggtt cggccgccag ccgcgcccgc gcatggcggt
      661 ggcggcgttg ttcatcctgc tctacgcgct gttccgcacc atggtggaat tcgtccgcct
      721 gcccgatgcg cagatcggct acctgttcgg caccggctgg atcaccatgg gcatgacgct
      781 gtgcgtgccg atgtggatcg ccggcgccat catcatgttc ctcgcctatc gcaggccggt
      841 gtacacgccg gccgcgcagc gcgtgctcga cgcgcagcag aaaggagcgg cctgaccatg
      901 cgccagtatc tcgacctgat gcgccacgtg ctggaaaccg gcacggccaa gaccgaccgc
      961 accggcaccg gcacgctgtc ggtattcggg caccagatgc gcttcgatct cgcccagggt
     1021 ttcccgctgg tcacgaccaa gaagctgcac ctgaagtcga tcgtctacga gctgctgtgg
     1081 ttcctgcgcg gcgagaccaa cacgcgttat ctcaaggaac acggcgtctc catctgggac
     1141 gagtgggccg acgacaacgg cgatctcggc ccggtctacg gttaccagtg gcgctcgtgg
     1201 cccacgcgcg acggccgcca catcgaccag atccagcagg tgctcgacca gctgaagaat
     1261 tcgccggact cccgccgcat catcgtttcg gcgtggaacg tgggcgagtt ggagcaaatg
     1321 gcgctgatgc cgtgccacgc gctgttccag ttctacgtgg ccgacggccg gctgtcgtgc
     1381 cagctgtacc agcgcagcgc cgatattttc ctcggcgtgc cgttcaacat cgccagctac
     1441 gcgctgctca cgttgatggt ggcgcaggtg tcgggcctga agccgggcga gttcatctgg
     1501 accggcggcg actgccacct gtacagcaac catctcgaac aggcgcgcac gcagctcgcg
     1561 cgcgcgccca tgccgctgcc gcgcatgaaa ctcaaccccg aggtgaagga cctgttcgcc
     1621 ttccgctacg aggatttcgc gctggaaggc tacgagtcgc atccgcacat caaggcgccg
     1681 gtggcggtgt gagggcgcgg ttttttcgac cgtccgtctg cctcgcgctg ccgtcggtga
     1741 cctgaaccgc tcgtcgtcag aatctgcaga ctgttgctgc acgccgcagg acgacgcggc
     1801 gcacgcggcg cgccgacatt cacggcgcat ggcgcttggc actatcaatc ccgtcacggc
     1861 ggcattaccg ccacctaccg ggagttgcca tgaaattcaa tacacgagtg ttctccgccg
     1921 cggccatcgt ggcctgcgct gcggcgctgt ccgcctgtgg tggcggcggc agttcttcca
     1981 gttccgccgc ggtttcgacc gcgccggggc agatcatcga caccagcaaa tgcctcgcca
     2041 ccgcgggtcc gttcgacgct gcgcagaacg ccatcgtcag cggcctgggc ggtctcacca
     2101 gttcgctgcc gcagggcgcc agcgtggctt cgtcgctgac caatgccgct tcgcacctgc
     2161 tcgacacgcc cgatgcggtg gtggcggcgc tgcagaatct tgcggccaac cacgatccgg
     2221 ccgcgttcgc ggccgaactc acctcggccg gcgacggcct gcgctgcggt cttgcctata
     2281 ccgattcggc gctgctcgac ctgacccagc agctgcagaa ccagtcggcc agcgtgccgg
     2341 cactgccctc gctgctcagc caggtggagg ccatcgagac catgctgggc cagggcctca
     2401 ccggccagac tgatctgacc aacctgacca acgcgctggg cgcgctgcag acgcagctgg
     2461 aacgcgtcaa ctcgcagctg ccggccgggt tcaagattcc caacatcagc ctgccgatgt
     2521 cgatgatcgg caacatcgcc acctcggctt cggtgatcct gcagagcgtg gcgcacctgg
     2581 acgccggcag cgcgcagggc gccatcaccg ggctggcgct gcaactggcc gggctggtca
     2641 acggctacag cgcgctgggc ctgcccggtg cggtggtgca gccgatctac agcctgctgc
     2701 tgtcaggcaa cacgcagttc gtgagcaaca tcgtgccgta catcggcccg ctgttcaccg
     2761 cgatcacccg cgtgctgcag tcgcagagca acccgctggg cacgctgcag cagatcgtca
     2821 tgggcggcct ctccggcgtg ccggcgagcg gcgccttcgc cagcctgctc aacctgctca
     2881 acggcggtgg caccagctcc accggcgcac agatcccgct gctctccgac ctgctggcac
     2941 tgctgattcc cggaggcgtg ccgctctgac agcggacggc ggcggtaccc gggcgcgcga
     3001 gcaatcgcgc gcctttttct ttgcacgctc actttaggaa cctcggaaga agcgtcgcga
     3061 gcgatggcag gttgcgcgag gacggagcgc agcaggcgga gcatacgttg aaggtatgtg
     3121 agcattgcga gcaccgccct cgcgcaaaat gcccagcgca gcagcttatt cagaggttcc
     3181 ttagaattcg cgcatgcacg acgccaccac catcagcctg
//
LOCUS       DASZZC010000444         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_614497,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000444 DASZZC010000000
VERSION     DASZZC010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614497"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1961
                     /locus_tag="VHE37_09385"
     CDS             <1..1961
                     /locus_tag="VHE37_09385"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HVT35785.1"
                     /translation="APRGFLRELSSVTVSTWRASRGFVPLVKLLASEEDVTRDALTAA
                     IDQAFAELYRHPLLRSTERITAYLRKKRLIPNEHSTEELIRFMVEQIVARSPVPVPDA
                     LVQEFWQFFNELFSSPELKGLGELTLDMVRLVIRTYEPMLVEIANLLKAGRRYNQWQL
                     TEVMRRAAMIRHDAVIVRRQIRALRYIKPFFETDPKDFRAQARIVAQMVREFGPFFVK
                     MAQVAAANADFLPDEIARELAVFHEDVPPMNEEEVNAAFMECYGKLPHKLYLDFDAAR
                     PVKSGSIGSVYFAKKPFVEDGVEVLRPVVIKVGRHNIDREFAIGKLVLGLAIMSSQYW
                     APHSKLAPFLRAMAEQVDEFIAGFMEELDFEAEAKNHLRFYERSLAGGNYRVPRLYSS
                     TRRILEMEYLADASSLTRTLARMPASERRRFQFQVGERLLYTVLHHIFVHREIHGDLH
                     PGNVMIGSDGALHLIDWGNVVSLDGKWNLVWDYLAGAVLADTDLLTDALIRVSTQPEE
                     NARRHDEIKALLTETLQKKGVRPLTRRNFVRELRQGGLEGLHRRGQTVLHLMSNTQQA
                     GLVLRRDYLHLSRALFAAAGSFGSLYENTPKRFLARDMAVSLARLPVVVTRQKVSGVA
                     MRWRERLARVLPLPRAVRARLAPALQAH"
     gene            1972..2985
                     /locus_tag="VHE37_09390"
     CDS             1972..2985
                     /locus_tag="VHE37_09390"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HVT35786.1"
                     /translation="MLYGVARNLLFRLDAERAHELTIAAFHHFPRLATAPFACRANIQ
                     PVRLMGLEFPSRVGLAAGLDKNGECIEAWFRLGFGFVEIGTVTPRPQPGNPKPRMFRL
                     PEHEALINRLGFNNKGVDHLLAQVAQAKVSGVLGINIGKNFDTPIERAADDYLACLHK
                     VYAAAGYVTVNISSPNTKNLRDLQDEARLDALLQQLVDARNELRQRHGKHTPLLVKIA
                     PDLVDEQIVQIARLARERGIDGLIATNTTVTRPQLESHTIAQQQGGLSGAPLRPQATH
                     VMRRLRAELGPDFPLIGVGGIADAAAAAERRAAGADLVQIYTGFIYRGPSLIAECAGA
                     LQH"
     gene            3081..>3409
                     /locus_tag="VHE37_09395"
     CDS             3081..>3409
                     /locus_tag="VHE37_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35787.1"
                     /translation="MRKTWSVLAAVGLGAFCVDQAAAVNAPDYDIPYVAAQYAHEFPD
                     SSRDAKNGEGYQLTAGMPLNFKFLPSKTALEVSFYDVARKRNLDGLKDYQSVISTDLV
                     KDFGLYG"
BASE COUNT          590 a         1103 c         1133 g          583 t
ORIGIN      
        1 acgcgccgcg cggtttcctg cgcgagctgt ccagcgtcac cgtttccacc tggcgcgcct
       61 cgcgcggctt cgtgccgctg gtgaagctgc tggccagcga agaggacgtg acgcgcgacg
      121 cactcaccgc cgccatcgac caggccttcg ccgagctcta ccggcacccg ctgctgcgct
      181 ccaccgagcg catcacagcg tacctgcgca agaagcggtt gatccccaac gagcactcga
      241 ccgaagagct gatccgcttc atggtcgagc agatcgtggc gcgctcgccg gtgccggtgc
      301 ccgatgcgct ggtgcaggag ttctggcagt tcttcaacga gctgttctcc tcgcccgagc
      361 tgaaagggct gggtgaactc acgctcgaca tggtgcggct ggtgatccgc acctacgagc
      421 cgatgctggt ggagatcgcc aacctgctca aggccggccg ccgctataac cagtggcagc
      481 tgaccgaggt gatgcgccgc gcggcgatga tccgccacga cgcggtgatc gtgcgccgtc
      541 agatccgcgc gctgcgctac atcaagccat ttttcgaaac cgatccgaaa gacttccgcg
      601 cgcaggcgcg catcgtggcg cagatggtgc gcgagttcgg cccgttcttc gtcaagatgg
      661 cgcaggtggc ggcggccaac gccgacttcc tgccggacga gatcgcgcgc gagctggcgg
      721 tgttccatga agacgtgccg ccgatgaacg aggaggaggt caacgccgcg ttcatggagt
      781 gctacggcaa gctgccgcac aagctgtacc tcgatttcga cgcagcgcgg ccggtgaagt
      841 cgggctcgat cggctcggtg tatttcgcca agaaaccttt cgtggaagac ggcgtcgagg
      901 tgctgcggcc ggtggtgatc aaggttggcc gacacaacat cgaccgcgaa ttcgccatcg
      961 gcaagctggt gctcgggttg gccatcatgt ccagccagta ctgggcgccg cacagcaagc
     1021 tcgcgccgtt cctgcgcgcc atggccgaac aggtcgacga gttcatcgcc ggcttcatgg
     1081 aggaactgga cttcgaggcc gaggcgaaaa atcacctgcg cttctacgag cgctcgctgg
     1141 ccggcggcaa ctaccgcgtg ccgcggctgt attcgtccac gcgccgcatc ctggagatgg
     1201 aatacctggc cgatgcgtct agcctcacgc gcacgctggc gcgcatgccg gcgagcgaac
     1261 gtcgccgttt ccagttccag gttggcgagc ggctgctcta caccgtgctg caccacatct
     1321 tcgtgcaccg cgaaatccac ggcgacctgc acccgggcaa cgtgatgatc ggcagtgacg
     1381 gcgcgctgca cctgatcgac tggggcaacg tggtgtcgct tgacggcaag tggaacctgg
     1441 tgtgggacta tctggccggc gcggtgcttg ccgataccga tctgttgacc gatgcgctga
     1501 tccgcgtcag cacgcagccg gaagaaaatg cgcggcggca cgacgagatc aaggcattgc
     1561 tcaccgagac gttgcagaag aaaggcgtga gaccgctcac gcggcgaaac ttcgtgcgcg
     1621 agctgcgcca gggcgggctg gaaggtttgc accgacgcgg gcagaccgtg ctgcacctga
     1681 tgtcgaacac gcagcaggct ggcctggtcc tgcgccgcga ttacctgcac ctgtcgcgcg
     1741 cgctgttcgc cgcggccggc agtttcggca gcctgtacga gaacacgccc aagcgtttcc
     1801 tcgcgcgcga catggcggtg tcgctggcgc gcctgccggt ggtggtgacg cggcagaagg
     1861 tgtctggcgt ggcgatgcgc tggcgcgagc gcctcgcgcg cgtgctgccg ttgccgcgcg
     1921 cggtgcgcgc gcgcctggcg ccagcgctgc aggcacattg agactgtgac gatgctctac
     1981 ggcgtcgcgc gcaacctgct tttccggctc gacgccgagc gcgcgcacga gttgaccatt
     2041 gccgcgttcc atcattttcc acggctggcc accgcgccat tcgcttgccg cgccaacatc
     2101 cagccggtgc ggctgatggg cctggagttt cccagccgcg tcgggctggc ggcggggctg
     2161 gacaagaacg gcgagtgcat cgaggcctgg ttccgtctcg gttttggctt cgtggagatc
     2221 ggtaccgtca cgccgcgacc ccagccgggc aatccgaagc cgcgcatgtt tcgcctgccg
     2281 gaacacgagg cgctgatcaa ccggctgggc ttcaacaaca agggtgtcga tcacctgttg
     2341 gcgcaagtgg cgcaggcgaa agtcagcggc gtgctcggca tcaatatcgg caagaacttc
     2401 gacacgccga tcgagcgtgc cgccgacgac tacctggcct gcctgcacaa ggtctatgcc
     2461 gcggccggct acgtgaccgt gaacatctct tcgcccaaca ccaagaacct gcgcgacctg
     2521 caggacgagg cgcgtctcga tgcgctgctg cagcagctgg tcgatgcgcg taacgagctg
     2581 cgccaacggc acggcaagca cacgccgctg ctggtgaaga tcgcgcccga tcttgtcgac
     2641 gaacagatcg tgcagatcgc gcggctggcg cgcgagcgcg gcatcgacgg gctgatcgcc
     2701 accaacacca ctgtcacgcg cccgcaactt gaatcccaca ccatcgcgca gcagcagggc
     2761 ggcttgtccg gcgcgccgct gcggccgcag gcgacccatg tgatgagacg gctgcgcgcg
     2821 gaactggggc cggatttccc attgatcggc gtgggcggca tcgccgatgc tgccgccgcc
     2881 gcggagcgcc gtgcggcggg agcggacctg gtgcaaatct acaccggctt catctaccgc
     2941 ggcccgtctc tcatcgccga gtgcgcggga gcgctgcagc actgagcgag ccggttgccc
     3001 ggcacgagac gatcagttaa tttgcgcgcg ctgtatcgct gcaacaattc atatacataa
     3061 acatcagcac ggggttggac atgcgcaaga catggagcgt tttggcggcg gtcggtttgg
     3121 gcgccttctg cgtggatcag gcggccgcgg tgaacgcgcc ggactacgac attccttacg
     3181 tggcagcgca gtacgcgcac gagtttcccg acagcagccg cgatgccaag aacggcgagg
     3241 gctatcagct cactgccggc atgccgctga acttcaagtt cctgccgtcg aagaccgcgc
     3301 tggaagtgag cttctacgac gtggcgcgca agcgcaatct cgacggcttg aaggattatc
     3361 agagcgtgat cagcaccgac ctggtgaagg attttggcct gtacggctg
//
LOCUS       DASZZC010000445         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_487875,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000445 DASZZC010000000
VERSION     DASZZC010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487875"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VHE37_09400"
     CDS             <1..575
                     /locus_tag="VHE37_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HVT35788.1"
                     /translation="SFVMVDIPGLIEGAAEGAGLGIRFLKHLQRTRLMLHLVDLLPPD
                     GADITANVRTINAELAAFSADLAAREQWLVFSKTDLMPRDEADALIARTLRQLKWKGR
                     WFAISAVTGAGCPQLCEAAMTWIEQGGKSSEPAAVKKSPAPARAKAAAKTAAEKKMEK
                     KSTKTVARRAAKKTPAKAKKRVTARRKSKL"
     gene            650..1774
                     /gene="proB"
                     /locus_tag="VHE37_09405"
     CDS             650..1774
                     /gene="proB"
                     /locus_tag="VHE37_09405"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HVT35789.1"
                     /translation="MLNRENIPQSRRWVIKVGSSLVTAKGQGLDHAAIADWCAQIAQL
                     RSAGRQIVLVSSGAVAEGMARLGLKKRPSVLHELQAAAAVGQMGLVRAYEDEFKKHGI
                     SAAQILLTHEDVADRGRYLNARSTFTTLVELGVVPVVNENDTVSTDEIRLGDNDTLGA
                     LTVNLIQGDLLVILTDQEGLYDSDPRLNPQAQLISHAELSDTRLTAMAGDSKGELGRG
                     GMRTKLSAAYTAARSGAATVIAHGRKPDALLRVARGEDIGTLLTPAQEPMAARKRWIA
                     GQLQLRGKLVLDDGAARVLRGQGRSLLPVGVKEVQGNFERGDLVACVDASGAEIARGL
                     CNYGAADARKIIGAKSEELAARLGYPGEKELVHRDNLVVN"
     gene            complement(1945..2208)
                     /gene="rpsT"
                     /locus_tag="VHE37_09410"
     CDS             complement(1945..2208)
                     /gene="rpsT"
                     /locus_tag="VHE37_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HVT35790.1"
                     /translation="MANIASAKKKARQAVKNRERNVAQRSMIRSLIKKTVKAVEAKDK
                     AAAQTAYAEMVPVLDRYADRGMIHKNKAARHKSRLAAHIKALG"
     gene            2297..3838
                     /gene="murJ"
                     /locus_tag="VHE37_09415"
     CDS             2297..3838
                     /gene="murJ"
                     /locus_tag="VHE37_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HVT35791.1"
                     /translation="MSSLLKSSSVVGLMTLVSRVLGLVREMMLTRMVGAGGGMDAFLV
                     ALMIPNFGRRMFAEGAFSQAFVPVFTETKTTRPHEEVQDLEAVVMGTLGGVLTLVTVI
                     GCLCAPLLVWMFASGFADDPAKELLSRGLLRWTFPYLMFISLTSMAGGVLNAYGRFAV
                     PAFTPVIFNLCIIASAWIDSGSVQALAWAVFAGGIFQFLFQLPSLARLRLLPRPYFSL
                     RDARVRRIVSLMLPMMLGSSVAQLSLLLNSNLSTHLGDGSVSWLYFANRLMEFPLGIF
                     SIAVGTAILPALSAHHATDSREHFSSTLDWSLRTILVIGVPAALGLTLLAGPLVATLF
                     GGGRFGERDVAMTTYALWAYAFGFLGFSFVKVLVPGFYARQQTALPARYAIISLVIGM
                     AVSLALFAWSRVQHWGAAHVALAFSTSLSAWINSALLYRKLRRDGVYTPAPQWGRYAL
                     QLALASIAMSGVVAWMSAPLSHWMAAGTWLRVGWTSEIIFAAIVVYASALWLCGLRPA
                     QFRRH"
     gene            3840..>4377
                     /locus_tag="VHE37_09420"
     CDS             3840..>4377
                     /locus_tag="VHE37_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HVT35792.1"
                     /translation="MEPGLELIRGRQNLSARHRGGVVTIGNFDGVHRGHQELIARARE
                     LAAGAPVTVLTFEPTPREFFTPQSAPPRISTLRGKLAALAAAGADRVLLQRFGHPFCE
                     LTPDAFVRELLVRQLGATAVVVGDDFRFGAKRAGDFALLRQLGQQHGFAAEQLATLVV
                     KGERCSSSALREALAAADL"
BASE COUNT          678 a         1445 c         1488 g          766 t
ORIGIN      
        1 gctcgttcgt gatggtggac attcccggcc tcatcgaagg cgcggcggaa ggtgctggtc
       61 tgggcatccg cttcctcaag cacctgcagc gcacccggct gatgctgcac ctggtcgacc
      121 tgctgccgcc cgacggcgcc gacatcaccg ccaacgtgcg caccatcaac gcggagctgg
      181 cggcgttctc ggccgacctc gcggcgcgcg agcagtggct ggtgttcagc aagaccgacc
      241 tgatgcccag ggatgaagcc gatgcgttga tcgcgcgcac gctgcggcag ctcaaatgga
      301 agggccgctg gttcgccatc tccgcggtga ccggcgccgg ctgcccgcag ctgtgcgagg
      361 cggcgatgac ctggatcgag cagggcggca agtcgtccga gccggctgcg gttaaaaaat
      421 cgccggcacc ggcacgggcg aaagcggcgg caaaaacggc cgctgagaag aagatggaaa
      481 agaaatcgac gaagacagtt gcgcgcaggg cggcgaagaa gacgcccgcc aaggccaaaa
      541 aacgagtcac cgcacgccgc aaatcgaagt tataaaaagt agtacaaata ttaaataatg
      601 ctacatttaa taacagacaa aatgccggtg cggtgaaggc aaaaatccca tgctgaaccg
      661 cgaaaacatt ccgcagagcc gccgctgggt gatcaaggtc ggcagctctc tggtcacggc
      721 caaaggccag gggctggatc acgccgccat cgccgactgg tgtgcgcaga tcgcgcagtt
      781 gcgcagtgcg ggccggcaga tcgtgctggt gtcgtcgggc gcggtggccg agggcatggc
      841 gcggctgggg ctgaagaagc gcccgagcgt gctgcacgag ttgcaggccg ccgctgccgt
      901 cggccagatg ggcctggtgc gcgcctacga agacgagttc aagaagcacg gaatttccgc
      961 tgctcaaatt ttgctcacgc atgaagatgt cgccgaccgc ggccgctatc tgaatgcgcg
     1021 cagcaccttc accacgctgg tggagctggg cgtggtgccg gtggtgaacg agaacgacac
     1081 cgtctccacc gacgagatcc gcctgggcga caacgacacg ctgggcgcgc tcacggtgaa
     1141 cctgatccag ggcgacctgc tggtgatcct caccgaccag gaaggcctgt acgacagcga
     1201 tccgcggctc aatccgcagg ctcagctgat ctcgcacgcc gagctgtccg acacgcggct
     1261 cacagcgatg gccggcgaca gcaagggcga gctcggccgc ggcggcatgc gcaccaagct
     1321 ttccgccgcg tacacggctg cgcgctcggg cgcggccacg gtgatcgcgc acgggcgcaa
     1381 gcccgacgcg ctgttgcgcg tggcgcgcgg cgaggatatc ggcacgctgc tcacgccagc
     1441 gcaggaaccg atggcggcgc gcaagcgctg gattgccggc cagctgcagc tgcgcggcaa
     1501 gctggtgctg gacgacggcg ccgcgcgcgt gctgcgcggg caggggcgca gcctgctgcc
     1561 ggtgggcgtg aaagaggtgc agggcaattt cgagcgcggc gacctggtgg cctgcgtcga
     1621 cgcctccggc gccgagatcg cacgcgggct gtgcaactac ggcgcggcag atgcgcgcaa
     1681 gatcatcggc gccaagagcg aagagctcgc cgcgcggctc ggttatccgg gcgagaagga
     1741 gttggtacac cgcgacaatc tcgtggtgaa ctgagggttg aatctcgccg gcgctgcgcg
     1801 ccgcccctct tttccaaaga ggataagcag tgcgcgctca ccttgagccc tcttcggaaa
     1861 agagggcagc tagcgtcagc gagcgggaga ttcccaaaca aaaacgccgg cacgaggccg
     1921 gcgttcgagt tgcagctggg agctttagcc cagtgccttg atgtgcgccg ccagacggga
     1981 cttgtgccgc gcggccttgt ttttgtgaat catgccgcgg tcggcgtagc ggtccagcac
     2041 cggcaccatc tccgcgtagg cggtctgcgc cgcagccttg tccttggcct ccactgcctt
     2101 gaccgtcttc ttgatcaggc tgcggatcat cgaccgctgc gcaacgttgc gctcgcggtt
     2161 cttcacggcc tggcgggcct tcttctttgc actggcgata ttggccaaag gtcactccaa
     2221 aagcggcgta aaaattaagg gcgcgcatta tccggacgcg tccgcccccc gtcaacagcg
     2281 gctaaactcc tcgtcgatgt cctccctgct caaatcctcc tccgtcgtcg gcctgatgac
     2341 gctggtctcg cgcgtgctgg gcctggtgcg cgagatgatg ctcacgcgca tggtcggcgc
     2401 cggcggcggc atggacgcct tcctggtggc gctgatgatc cccaacttcg gccgccgcat
     2461 gtttgctgaa ggcgccttct cgcaggcctt cgtgccggtg ttcaccgaga ccaagaccac
     2521 gcgcccgcac gaggaagtgc aggacctgga ggcggtggtg atgggcacgc tcggcggcgt
     2581 actcacgctg gtcaccgtga tcggctgcct gtgcgccccg ctgctggtgt ggatgttcgc
     2641 ctcgggcttc gccgacgacc cggccaagga actgctctcg cgcggcctgc tgcgctggac
     2701 ctttccctac ctgatgttca tctcgcttac ctcgatggcc ggcggcgtgc tcaacgccta
     2761 cggccgcttc gcggtgcccg cgttcacgcc ggtgatcttc aacctgtgca tcatcgcctc
     2821 ggcctggatc gactccggct cggtgcaggc gctggcctgg gcggtgttcg ccggcggaat
     2881 tttccagttc ctgttccagc tgccgtcgct ggcgcgcctg cgcctgctgc cgcggccgta
     2941 tttctcgctg cgcgacgcgc gcgtgcggcg catcgtctcg ctgatgctgc cgatgatgct
     3001 gggctcgtcg gtggcgcagc tgtcgctgct gctcaattcc aacctgtcca cgcacctggg
     3061 cgacggctcg gtcagctggc tgtatttcgc caaccgcctg atggaatttc cgctggggat
     3121 attctcgatc gcggtcggca ctgcgatcct gccagcgctc tcggcgcacc acgccaccga
     3181 ctcgcgcgag catttttcct ccacgctgga ctggagcctg cgcaccatcc tggtgatcgg
     3241 cgtgccggcg gcgctggggc tcacgctgct cgccgggccg ctggtggcca cgctgttcgg
     3301 cggcggccgc ttcggcgagc gcgacgtggc catgaccacc tacgcgctgt gggcctacgc
     3361 cttcggcttc ctgggttttt ccttcgtcaa ggtgctggtg ccgggtttct acgcgcgcca
     3421 gcagaccgcg ctgccggcgc gttacgccat catcagtctg gtcatcggca tggcagtgag
     3481 cctcgcgctg ttcgcgtggt cgcgcgtgca gcactggggc gcggcgcacg tggcgctggc
     3541 gttctccacc tccctgtcgg cctggatcaa ctcggcgctg ctgtaccgca agctgcgccg
     3601 cgacggtgtg tacacgcccg cgccgcagtg gggccgctac gcgctgcagc tggcgctggc
     3661 cagcatcgcc atgtccggcg tggttgcgtg gatgagcgcg ccgctgtcgc attggatggc
     3721 ggcgggaacc tggctgcgcg tgggctggac cagtgaaatc attttcgccg cgatcgtggt
     3781 ctacgccagc gcgctctggc tctgcggcct gcggcccgcg cagttccggc gccactgaca
     3841 tggaaccggg actcgaactg atccgcggcc gtcagaacct ttccgcacgc catcgcggcg
     3901 gcgtggtgac catcggcaat ttcgacggcg tgcatcgtgg ccaccaggag ctgatcgcgc
     3961 gggcgcgcga actcgcggcc ggcgcgccgg tcaccgtgct caccttcgag cccacgccgc
     4021 gcgaattctt cacgccgcaa tcggcaccgc cgcgcatctc cacgttgcgc ggcaagctgg
     4081 cggcgctggc cgccgcgggt gccgatcgcg tgctgctgca gcgcttcggc catccattct
     4141 gcgaactcac gccggacgcc ttcgtgcgcg aactgctggt gcgccagctc ggcgcgacgg
     4201 cggtggtggt cggcgatgac ttccgcttcg gtgcaaaacg cgccggtgac ttcgcgctgc
     4261 tgcggcagtt gggccagcag catggattcg cggccgagca gctggccacg ctggtggtga
     4321 agggcgagcg ctgcagttca tcggcactgc gcgaggcgct ggcggctgcg gatctcg
//
LOCUS       DASZZC010000446         4217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1191859,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000446 DASZZC010000000
VERSION     DASZZC010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191859"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            512..>4217
                     /locus_tag="VHE37_09425"
     CDS             512..>4217
                     /locus_tag="VHE37_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HVT35793.1"
                     /translation="MNADTQDASGREQEMLLAQLDALMEAAAPRSKQALLRSFVRQYY
                     EPASYDALKLRTPAELVATALAHFRLVEKRAAGEAIVQVLPPDPASASQFAVVRTGVD
                     DMPFLVDSISMAVRDAGSVIDWAVHPVLRFRRNAANAITAVYSAQEAEGGPESLIHAE
                     IDPLPSPEAYAALEQNIREVLADLRVVVSEFEPMRAKAREVADSLLQVPEGADADEFA
                     EAREFLLWLEGERFTFLGYSETELRTGKDGKSTFVNKPAAALGLARKGGRFADAEQLI
                     APKAELDKYTDSPRLVVITKANQKSPVRHAEYMDVVSVKRYAADGTLKGTSRFLGLFS
                     VEAYIERPREIPLIRRKAAYVMAHSHVPEDSYGGKALRLILHTLPRDELFQSSEEQLL
                     ETAMSIRALRDRHQLKLFLRRDRYGRSYSAMVYLPRERYSRELRDRVGAELMGICGGS
                     AIERHVEFPRGAQARIYYIVRTPPGTTLSLTTAEIEKRLIAVTRSWRDQLREILLAQP
                     GKDSALLAARYADAFPLSYAEHATAAQAEEDLRYLARLSPSQRLLVNAGGRPQDLKLY
                     SWSRPVGLSDVLPILENFGLRTVRQDPTEITPKDGESLWLQTFEITAALTAPPTAEQK
                     AAFETAFLKCWAGEIENDGLNRLVLGAGLDWRQVTAVRLVTKYLIQTGLPYSQSYMEQ
                     MLGDHAAIARLLVQLFEARFDPALDDARRQAEEIKLAQAIDAALDKVASLDADRVLRA
                     FLSVVRAGLRTNYYQSNATGEKKTYVSLKIDSSKVPELPLPRPLFEIFVYAPEVEGVH
                     LRGGKVARGGLRWSDRRQDFRTEVLGLMKAQQVKNTVIVPVGAKGGFVVKKPVDPANR
                     DAWLAQGIECYKTFVRGLLDVTDNRVAGKIAPPMNVVRHDPDDPYLVVAADKGTATFS
                     DIANGLSAEYSFWLGDAFASGGSAGYDHKKMGITAKGAWESVKRHFREAGKNIQEEEF
                     SVVGIGDMSGDVFGNGMLLSRKIRLIAAFDHRHIFLDPNPDCEKSFAERERMFALPRS
                     SWADYDVKLISKGGGVHARNLKSIKLTDEVKKALDVRDNAMTPAELMKAILKAPVDLL
                     WNGGIGTYVKGHNQAHQDVGDRANDAIRVNGRELRCKVVGEGGNLGFTQLGRVEYALA
                     GGRLNTDAIDNAGGVHSSDREVNIKIPLNELMAHGKLSRKERDPLLVKMTGDIARFVL
                     RDNYVQSAAVSMLERNAPARLDEHAELIRLL"
BASE COUNT          777 a         1369 c         1417 g          654 t
ORIGIN      
        1 aatgctggtt ggccttctcg gtcacgccgc cgaacaggca ggggttgctg ccgctgccgt
       61 catacgccag ctggcgctgc tcgacctcgg cggtcgacgc gcagccttgc aggagcgcca
      121 agcccagcag cgccgccgtc atcatcaggt aattcttcat tgccgcctcc gtccggacgc
      181 aagggcggcg atgtcgggtt tccgtcgtcg cggcttgcgc gagcagcgac gagcaattga
      241 cggaccccgc gtggaccagc ggtgaagaga tagatgattt gtccacggcg cggtgcatag
      301 cgcggcggcg gcaaaaaaac cgtccaccgc accggcaggg gcgatgttat aaaaaattat
      361 atatagttaa atatattaaa taataaataa catgataaag ggcagtgcgg tggaggcaaa
      421 aaatgccgcg cagcgcttcg gattaagatg cccgctccaa aaaaaaggat ggccgggccg
      481 cgcacgcggt ctgtaagccg gaggaaatgc gatgaacgcc gatacgcagg atgcgtccgg
      541 gcgcgagcag gaaatgctgc tggcccagct cgacgcattg atggaggctg ccgcacccag
      601 gtcgaagcag gcgctgctcc gcagcttcgt ccgccagtac tacgaacccg cctcgtacga
      661 cgcgctcaag ctgcgcacgc cggccgaact ggtcgccacc gcgctggcgc atttcaggct
      721 ggtggagaag cgcgccgccg gcgaggccat cgtgcaggtg ctgccgccgg acccggccag
      781 cgcttcgcag ttcgccgtgg tgcgcaccgg cgtcgatgac atgccgttcc tggttgactc
      841 catcagcatg gcggtgcgcg atgccggcag cgtgatcgac tgggcggtgc acccggtgct
      901 gcgcttccgc cgcaatgcgg ccaacgccat caccgcggtc tattccgcgc aggaagccga
      961 gggcggtccc gaatcgctga tccacgccga gatcgacccg ctgccatcgc ccgaggctta
     1021 cgcggcgctg gagcagaaca tccgcgaggt gctggccgac ctgcgcgtgg tggtgagcga
     1081 gttcgagccg atgcgcgcca aggcgcgcga ggtggccgac tcgctgctgc aggtgcccga
     1141 gggcgccgac gccgacgagt tcgccgaggc gcgcgagttc ctgctgtggc tggaaggcga
     1201 gcgcttcacc tttctcggct actcggaaac cgagctgcgc accggcaagg acggcaagtc
     1261 caccttcgtc aacaagccgg ccgccgcgct ggggctggcg cgcaagggcg gccgttttgc
     1321 cgatgccgag cagctgatcg cgcccaaggc cgaactggac aagtacaccg attcgccgcg
     1381 gctggtggtg atcaccaagg ccaaccagaa aagcccggtg cgccacgccg agtacatgga
     1441 cgtggtgtcg gtgaagcgct atgccgccga cggcacgctc aagggcacgt cgagattcct
     1501 cgggctgttc tcggtggagg cgtatatcga gcggccgcgc gagatcccgc tgatccggcg
     1561 caaggccgcc tacgtgatgg cgcactcgca cgtgccggag gactcctatg gcggcaaggc
     1621 gctgcgcctg atcctgcaca cgctgccgcg cgacgagctg ttccagtcca gcgaggagca
     1681 gttgctggaa accgccatgt cgatccgcgc gctgcgcgac cggcaccagc tcaagctgtt
     1741 cctgcgccgc gaccgctacg gccgttccta ctcggccatg gtctacctgc cgcgcgagcg
     1801 ctactcacgc gaactgcgcg accgggtcgg cgcggaactg atgggcatct gcggcggcag
     1861 cgccatcgag cggcacgtgg aatttccgcg cggcgcgcag gcccgcatct actacatcgt
     1921 gcgcacgccg ccgggcacca cgttgtcgct gaccaccgca gagatcgaga agcggctgat
     1981 cgcggtgacg cgcagctggc gtgaccagct gcgcgagatc ctgctggcac agccgggaaa
     2041 ggacagcgcg ctgctggccg cgcgctacgc cgacgccttc ccgctgtcgt atgccgagca
     2101 cgcaaccgcc gcgcaggcgg aggaagacct gcgttatctc gcgcggctgt cgcccagtca
     2161 gcgcttgctg gtcaatgccg gcggccggcc gcaggacctg aagctttatt cctggagcag
     2221 gccggtcggc ctgtccgacg tgctgcccat cctggaaaac ttcggcctgc gcacggtgcg
     2281 ccaggacccg acggagatca cgccgaagga tggcgagagc ctgtggctgc agaccttcga
     2341 aatcaccgcg gcgctcaccg cgccaccgac agcggagcag aaagcggcgt tcgagaccgc
     2401 cttcctcaaa tgctgggctg gtgaaatcga aaacgacggg ctgaatcgcc tggtgctcgg
     2461 cgccgggctg gactggcgcc aggtcacggc ggtgcgcctg gtcaccaagt acctgatcca
     2521 gaccgggctg ccatattcac agtcctacat ggagcagatg ctgggcgacc atgcggcgat
     2581 cgcgcgcctg ctggtgcagc tgttcgaggc gcgcttcgat cccgcgctcg acgacgcgcg
     2641 gcgccaggcc gaggagatca agctggcgca ggccatcgac gccgcgctgg acaaggtcgc
     2701 ctcgctagac gccgaccgcg tgctgcgcgc gttcctttcg gtggtgcgcg cgggcctgcg
     2761 caccaattac taccagagca atgccaccgg cgagaagaag acctacgtct cgctgaagat
     2821 cgattcgagc aaggtgccag aactgccgct gccgcggccg ctgttcgaga tcttcgttta
     2881 cgcgccggag gtggaaggcg tgcacctgcg cggcggaaaa gttgcgcgcg gcggcctgcg
     2941 ctggagcgat cgccggcagg atttccgcac cgaggtgctg ggcctgatga aggcgcagca
     3001 ggtgaagaac acggtgatcg tgccggtcgg cgccaagggc ggtttcgtgg tgaagaagcc
     3061 agtcgatccg gccaaccgcg atgcctggct ggcgcagggc atcgagtgct acaagacttt
     3121 cgtgcgcggc ctgctcgacg tcaccgacaa ccgcgtcgcc ggaaagatcg cgccgcccat
     3181 gaatgtcgtg cgccacgatc ctgacgaccc gtatctcgtc gtcgccgccg acaagggcac
     3241 ggccactttt tccgatattg caaacggcct gtcggccgag tattccttct ggctcggcga
     3301 cgccttcgcc tccggcggca gcgccggcta tgaccacaag aagatgggca tcaccgccaa
     3361 gggcgcgtgg gagagcgtca agcgccactt ccgcgaagcg gggaagaata tccaggaaga
     3421 ggaattcagc gtcgtcggca tcggcgacat gagcggcgac gtgttcggca acggcatgct
     3481 gctgtcgcgc aagatccgtc tgatcgccgc gttcgatcac cgccacattt ttctcgaccc
     3541 caatcccgac tgcgaaaaga gctttgccga gcgcgagcgc atgttcgcgc tgccgcgctc
     3601 gtcgtgggcc gattacgacg tgaagctcat cagcaagggc ggcggcgtgc atgcgcgcaa
     3661 cctcaagtcg atcaagctga ccgacgaagt gaagaaggcg ctcgacgtgc gcgataacgc
     3721 aatgacgccg gccgagctga tgaaggccat cctcaaggcg ccggtcgacc tgctgtggaa
     3781 cggcggcatc ggcacctatg taaagggaca caaccaggcg caccaggatg tcggtgaccg
     3841 cgccaacgac gccatccgcg tcaatggccg cgagctgcgc tgcaaggtgg tgggcgaggg
     3901 cggcaatctc ggcttcaccc agctgggccg cgtggaatat gcgcttgccg gcgggcgcct
     3961 caacaccgac gccatcgaca atgccggtgg cgtgcacagc agcgaccgcg aggtgaacat
     4021 caagatcccg ctcaacgagc tgatggcgca cggcaagctc tcgcgcaagg agcgcgaccc
     4081 gctgctggtg aagatgaccg gcgacatcgc ccgcttcgtg ctgcgcgaca actacgtgca
     4141 gtcggcggcg gtgtccatgc tcgagcgcaa cgccccggca cggctggatg aacacgcgga
     4201 gctgatccgc ctgctgg
//
LOCUS       DASZZC010000447         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_614674,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000447 DASZZC010000000
VERSION     DASZZC010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614674"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VHE37_09430"
     CDS             complement(<1..519)
                     /locus_tag="VHE37_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT35794.1"
                     /translation="MSIPAIEFELPAAEADAARAKYRRNWLGFMAMTVAVFMAFLDIQ
                     VVASSLNQIQAGLSATAQEISWVQSAYIIAEVIAIPLSGYLSRILSTRVYFTLSVLGF
                     TLASAACAMAWNIESMMLFRAIQGFLGGGMIPSSFAALYIMFPPDKRIVPQVLAGLVA
                     TSAPTLGPVLGGW"
     gene            complement(598..1617)
                     /locus_tag="VHE37_09435"
     CDS             complement(598..1617)
                     /locus_tag="VHE37_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HVT35795.1"
                     /translation="MKVLGADLGFGYTKATDGRQYQVFKSVVGEATDVTFAEQLLPGA
                     AQHARHLEVGGRGYFVGELAEAQSRGRGFTLDQTQFLTQFCKILALSALAPFAETNQP
                     IRLVTGLPISFFRRHKDALTALLQNRHPVVIIKANGEREEKNIYIEKVRVIPQPFGSM
                     FNLMLNDLGKAASQRFISEKIGIIDIGFRTADYTISDKTKFSERGSQSTDHGIAAAYT
                     AIANLLQEKSGVNIELYRLYEAVAKGSIKIKGKRYDLNEVIKSAFQQLSAKIAAEVMR
                     LWADDWDLDAIVITGGGGAVLAPYLAPLLEGEVLPMPPDVDARLNNVLGYWKYGMNIW
                     PPQGG"
     gene            1726..2607
                     /locus_tag="VHE37_09440"
     CDS             1726..2607
                     /locus_tag="VHE37_09440"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HVT35796.1"
                     /translation="MKILRTPDQRFAGLPGYPYAPHYAQLGELRMHYVDEGPRDGAVV
                     LMLHGEPSWSYLYRRMIPPFVAAGYRVLAPALIGFGKSDKPSRMADYSYQRHVDWLLA
                     WIEQLDLTNVTLIVQDWGSLLGLRIAALHGARFSRIVVANGFLPTGTGASKGFRRWRA
                     FATWSPWFPIGRIVGWGCLSKLSPEVIAAYDAPFPSAKYKAGARAFPRLVPMEPDNVA
                     VPENRKAWDALGLWNKPFLTVFGKRDPILGRSDKPLQEHVPGARGQPHRRINGSHFVQ
                     EDCGEELAQIVLDWLKG"
     gene            complement(2708..3100)
                     /locus_tag="VHE37_09445"
     CDS             complement(2708..3100)
                     /locus_tag="VHE37_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT35797.1"
                     /translation="MKHLYPIITTASVRECRDFYVHALGARVLFEQDWYVHLSIDGWE
                     IGFLSPRNSVRLPVFRHATQSRGLCIALEVGNVRQMYEEFQRKDIEIFGPLEELAGGE
                     LVFSVMDPSGAVINIVERSPDAAQMVEI"
     gene            complement(3097..3498)
                     /locus_tag="VHE37_09450"
     CDS             complement(3097..3498)
                     /locus_tag="VHE37_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVT35798.1"
                     /translation="MDKVTHRGGCHCGAVRFEVRAPARVTVQHCNCSMCSKTAFVHLI
                     VPKTDFRLVAGAEQLALYTFNTGVAQHLFCKVCGVKSFYIPRSNPDGFSVNLRCFDDG
                     TLEQVNYENFDGQNWEAHGAKLAHLSQGGGA"
BASE COUNT          638 a         1153 c         1204 g          632 t
ORIGIN      
        1 ccagccgccg agcaccgggc ccagcgttgg cgcgctggtg gcgaccaggc cggcgagcac
       61 ctgcggcacg atgcgcttgt ccggcggaaa catgatgtac agagcagcga acgagctcgg
      121 tatcatgccg ccgccgagaa atccctggat ggcgcggaac agcatcatcg attcgatgtt
      181 ccaggccatg gcgcaggccg cgctggccag cgtgaagccg agcaccgaca gcgtgaagta
      241 gacgcgcgtg gagaggatgc gcgagagata gccagacagc gggatggcga tgacttccgc
      301 gatgatgtag gccgactgca cccaggagat ttcctgcgca gtggccgaca ggcccgcctg
      361 gatctggttg agcgaactgg cgacgacctg gatgtcgaga aaggccatga acacggccac
      421 cgtcatggcc atgaagccga gccagttgcg gcggtacttc gcacgcgccg cgtcggcctc
      481 ggccgcaggc agttcgaact cgatcgcggg gatgctcatg gaggcgcgca gcttacgggc
      541 tgcgcaacac gagcgggtcg tccttgcgga ttaagcgctc gccggcgtca gccgccctca
      601 accaccttga ggcggccaga tgttcatgcc gtacttccag tagccgagca cgttgttcag
      661 gcgcgcgtcg acatccggcg gcatcggcag cacttcgcct tcgagcagcg gcgccaggta
      721 cggcgccagc accgcgccgc cgccgccggt gatgacgatg gcgtcgagat cccagtcgtc
      781 ggcccacagg cgcatgacct cggcggcgat cttggccgac agctgctgga acgccgactt
      841 gatcacctcg ttgaggtcgt agcgcttgcc cttgatcttg atgctgccct tggccaccgc
      901 ctcgtacaaa cggtacagct cgatgttcac gccgcttttt tcctgcagca ggttggctat
      961 ggcggtgtag gcggcggcga tgccgtggtc ggtggactgg ctgccgcgct cggagaactt
     1021 ggtcttgtcg ctgatggtgt agtcggcggt gcggaagccg atgtcgatga tgccgatctt
     1081 ctccgagatg aagcgctggc tggcggcctt gcccaggtcg ttgagcatca ggttgaacat
     1141 gctgccgaag ggctgcggga tcacgcgaac cttctcgatg tagatgttct tctcttcgcg
     1201 ctcgccgttg gccttgatga tgaccaccgg atggcggttc tgcagcagcg cggtgagcgc
     1261 gtccttgtgg cggcggaaga agctgatcgg caggccggtg accaggcgga tcggctggtt
     1321 ggtctcggcg aagggcgcca gcgccgacag cgccaggatc ttgcagaact gggtgaggaa
     1381 ctgggtctgg tccagggtaa agccgcggcc gcgcgactgc gcctcggcca gctcgccgac
     1441 gaaatagccg cgtccgccca cttccaggtg gcgcgcgtgc tgcgcggcgc cgggcagcag
     1501 ctgctcggcg aaggtcacgt cggtggcttc gccgaccacg gacttgaaca cctggtactg
     1561 ccggccgtcg gtggccttgg tgtaaccaaa gccgagatcg gcgcctagga ctttcacggt
     1621 ggaaccccgc agatattttt gtgcccggtt tttagcatgc agccgggggg cggtcaaaga
     1681 atcgcaccgt cataatgcgc gcggaaaacg ggaagggaaa aacacatgaa aatcctgcgc
     1741 acgccggacc agcgctttgc cggcctgccc ggctacccct acgcgccgca ctacgcgcag
     1801 ctgggcgaac tgcgcatgca ctatgtcgac gaaggcccgc gcgacggcgc cgtcgtgctc
     1861 atgctgcacg gcgagccgag ctggagctat ctctaccgca ggatgatccc gccgttcgtg
     1921 gcggcgggct accgcgtgct ggcgccggcc ctgatcggct tcggcaagag cgacaagcct
     1981 tcacgcatgg ccgactacag ctaccagcga cacgtggact ggctgctggc gtggatcgaa
     2041 cagcttgatc tgaccaacgt caccctgatc gtgcaggact ggggctcgct gctgggcctg
     2101 cgcatcgcgg cgctgcacgg cgcgcgcttt tcgcgcatcg tggtggcgaa cggatttttg
     2161 cccaccggta ccggcgcgtc gaaaggcttc cgccgctggc gcgcgttcgc cacctggtcg
     2221 ccgtggtttc ccatcggccg catcgtcggc tggggctgcc tgagcaaact gtcgccggag
     2281 gtgatcgccg cctacgacgc gccgtttccc tcggcgaagt acaaggccgg cgcgcgcgcc
     2341 ttcccgcgcc tggtgccgat ggagccggac aacgtcgccg tgccagagaa caggaaagcg
     2401 tgggacgcgc tgggactatg gaacaagccc ttcctcaccg tgttcggcaa gcgcgacccg
     2461 atcctcggcc gctccgacaa gccgctgcag gaacacgtgc ccggtgcgcg cggccagccg
     2521 caccggcgca tcaacggcag ccacttcgtg caggaggatt gcggcgagga gcttgcgcag
     2581 atcgtgctgg actggctcaa gggctgaatt tcgccctgcc gcaccccggg ttttgatgtt
     2641 ataaattatt atacaaaaat acaataaata aataatttat aacatcaaag atggcggtgc
     2701 ggtggagcta gatttcaacc atttgcgcgg catcgggcga gcgctcgacg atgttgatca
     2761 cggcgcccga cgggtccatc accgagaaca ccagctcgcc gccggccagt tcttccagcg
     2821 ggccgaagat ctcgatgtcc ttgcgctgga actcctcgta catctggcgc acgttgccca
     2881 cttccagcgc gatgcacagc ccgcgcgact gcgtggcgtg gcggaacacc ggcaggcgca
     2941 cggagtttct cggcgagagg aatccgatct cccagccgtc gatcgacagg tgcacgtacc
     3001 agtcctgctc gaacagcacg cgcgcgccca gcgcgtgcac gtagaaatcg cggcactcgc
     3061 gcaccgacgc ggtggtgatg atggggtaca ggtgcttcat gcgccgcctc cctgcgacag
     3121 gtgcgcaagc ttcgcgccat gcgcttccca gttctgcccg tcgaagtttt cgtagttcac
     3181 ctgttccagc gtgccgtcgt cgaagcagcg caggttgacc gagaagccgt cgggattgga
     3241 gcgcggaatg tagaacgact tcaccccgca caccttgcag aacaggtgtt gcgcaacgcc
     3301 ggtgttgaag gtgtagagcg ccagctgctc ggcgccggcg acgagcctga agtcggtctt
     3361 gggcacgatc aggtgcacga aggcggtctt gctgcacatc gagcaattgc agtgctgcac
     3421 ggtcacccgc gcgggcgcgc gcacctcgaa gcgcacggcg ccgcaatgac atccacccct
     3481 gtgtgtgacc ttgtccacgc cgtccctccc cgcggcgaca agcttagcca atgaagcggc
     3541 gcgccgctgc tcccggcagg gtgagtacgg ccagcacggt cacgatgaac ggcccggccg
     3601 gctggttctt gatgccggcc agcagtc
//
LOCUS       DASZZC010000448         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_98033,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000448 DASZZC010000000
VERSION     DASZZC010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98033"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VHE37_09455"
     CDS             complement(<1..1146)
                     /locus_tag="VHE37_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HVT35799.1"
                     /translation="MMPKSRFNSFMSRLLLAAAAATALAPATASAWWNKDWAYRKEIT
                     LDAGDKGIKLGEDLHDAPVLIRLHEGVLHFTDTKDDGSDLRFVAGDDKTPLKFHIEKF
                     DAVFNLGFVWVRVPELKKGQQTKLWMYYGNPNATAGDEPAASYDSAQVLDYHFGEKGG
                     MALDTTAYKNNATSAVAIQESGLIGNAAKFDGNTALLLPQTAALNQASGAPMTWSAWI
                     KPAAAEQDAVIYAHFHGGSGLVVGLEKGQPYVRIIDESGQVRQSPPMAAIAANSWHHL
                     ALVTSGQATLYLDGAAGPSMPAALPLLDGAASLGGQAVNGGVMAGFRGEIDELEIANV
                     ARSAALLALLAVNQGEGDRLVSFGADEAPSSWSSGYVGIILGSVTLDG"
     gene            complement(1160..2758)
                     /locus_tag="VHE37_09460"
     CDS             complement(1160..2758)
                     /locus_tag="VHE37_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HVT35800.1"
                     /translation="MDRQLAGNPRAWAAAGIALLIHCAVVSAAPAPAPVRHIDIWEFD
                     IDGNSVLDEATITDAVSRYTGPERTADDVDAARASLEAAYKQRGYRTVSVSIPKQSVR
                     EGIVRLQVNESHIEHLNVIGSKYHSIDQIKELAPSLAEGEVPDFTRVQHDLAALNQQA
                     DRRVTPALKAGNAPGTVDVDLVVDDKLPLHGSLELNNRQSQSTTPLRATAALSYDNLW
                     QRGHSLSLSYNVAPRRSSDSGVWFGSYLMHFSDTSFNLLFNALKSDSNVSTVGGTEVL
                     GTGRMFGLRGVWQLSASEGFYSSATFGADYKHFNTVTMLGSSQLPTPVTYVPFTIGYS
                     AMWRGAAVTQFDGSLMYTAAWIGSDEATLDLNRLSARGNMLSLRLSLATTRPLVADSQ
                     LYAAVHTQLTDQPLISNEQFSAGGMDSVRGYYEAETLGDYGFHATTELRSPSMIDALP
                     FGPYPAGEFRGFAFVDGSVLRLRPPVNAGQGDQTSLASFGAGFNFTLSPAFVSSLVWA
                     DPLIDGSATRAWSSRFLFRVATSF"
     gene            complement(3093..3464)
                     /locus_tag="VHE37_09465"
     CDS             complement(3093..3464)
                     /locus_tag="VHE37_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35801.1"
                     /translation="MDRSVGIWIFFVASVGLLVYALFGSHGDDAPAHTYSSASPAAPV
                     AAAPAVPPGRAQPQPQAMHAGTAPVPATAPTTGSATRPPVPMGAPENDTESDDCSQMP
                     LPPGCEPDTPAPPESSDDDAQ"
     gene            complement(3543..>3809)
                     /locus_tag="VHE37_09470"
     CDS             complement(3543..>3809)
                     /locus_tag="VHE37_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVT35802.1"
                     /translation="KRRAEAVVRILEAIARARDENHVDVRGYYHWSLTDNFEWGKYGP
                     RFGLYTVDVLTDPRLQRKPTAAVDAYRQIIRDGGVPAGYRPLLG"
BASE COUNT          602 a         1247 c         1305 g          655 t
ORIGIN      
        1 gccgtccagc gtgaccgaac ccaaaatgat gccgacgtaa cccgagctcc agctcgaggg
       61 cgcttcgtcc gcgccgaagc tcaccagcct gtcgccctcg ccctggttga cggcaagcag
      121 cgccagcagc gccgcggaac gcgccacgtt ggcgatctcc agttcatcga tctcgccacg
      181 gaatccggcc atcacgccgc cgttgaccgc ctggccgccg agcgacgcgg cgccatcgag
      241 cagcggcagc gccgccggca tcgacggccc tgccgcgcca tcgaggtaca gcgtggcctg
      301 tccgctggtg accagcgcca ggtgatgcca gctgttcgcg gcgatggcag ccatcggcgg
      361 cgactgccgc acctggccgg actcgtcgat gatgcgcacg tagggctggc ccttctccag
      421 cccgaccacc agccccgaac cgccatggaa gtgtgcgtag atcaccgcgt cctgctcggc
      481 cgcggccggc ttgatccacg ccgaccaggt catcggcgcg ccggaagcct ggttgagcgc
      541 ggccgtctgc ggcagcagca gcgcggtgtt gccgtcgaac ttcgcggcat tgccgatcag
      601 cccgctctcc tggatcgcca ctgccgacgt cgcgttgttc ttgtaggcgg tggtgtccag
      661 cgccatgccg cccttctcgc cgaagtggta gtcgagcacc tgcgccgagt cgtagctggc
      721 cgccggctcg tcaccggccg tcgcgttggg gttgccgtaa tacatccaca gcttggtctg
      781 ctggcccttc ttcagttcgg gcacgcgcac ccagacgaag cccaggttga acaccgcatc
      841 gaatttttcg atgtggaact tgagcggcgt cttgtcgtcg ccggcgacga agcgcaggtc
      901 gctgccgtca tccttggtgt cggtgaaatg cagcacgcct tcgtgcaggc ggatcagcac
      961 cggcgcgtcg tgcaggtctt cgcccagctt gatgcccttg tcgccggcat cgagcgtgat
     1021 ctctttgcgg taggcccagt ccttgttcca ccaggcggag gccgtggccg gcgccagcgc
     1081 ggttgccgcc gcagcggcga gcagcagccg cgacatgaaa gaattgaatc gggatttcgg
     1141 catcatggtc gggcctggct tagaacgacg tggcgacacg gaacaggaaa cggctggacc
     1201 acgcacgcgt ggcgctgccg tcgatcaggg gatcagccca taccagtgag ctgacaaaag
     1261 cgggagacag ggtgaagttg aagccggcgc cgaaactggc cagcgaggtc tggtcgccct
     1321 ggccggcgtt caccggcgga cgcaggcgca acaccgaacc gtcgacgaag gcgaatccgc
     1381 ggaactcgcc cgccgggtac ggaccgaacg gcagcgcgtc gatcatcgac gggctgcgca
     1441 gttcggtggt ggcgtggaag ccgtagtcgc ccagcgtttc ggcttcgtag tagccgcgca
     1501 ccgagtccat gccgccggcg ctgaactgct cgttggagat cagtggctgg tcggtcagct
     1561 gcgtgtgcac cgctgcatac agctggctgt cggcgaccag gggccgcgtg gtggccagcg
     1621 acaggcgcag gctgagcatg ttgccgcgcg cactcaggcg gttgaggtcg agcgtggctt
     1681 cgtcgctgcc gatccaggct gcggtgtaca tcaggctgcc gtcgaactgc gtcaccgccg
     1741 cgccgcgcca catcgcggaa tagccgatgg tgaacggcac gtaggtcacc ggcgtcggca
     1801 gctgcgaact gccgagcatg gtcaccgtgt tgaagtgctt gtagtcggcg ccgaaggtgg
     1861 cggaggaata gaagccttcg ctggccgaca gctgccacac gccgcgcagg ccaaacatgc
     1921 ggccggtgcc gagcacctcg gtgccgccga cggtgctgac gttgctgtcc gacttcagcg
     1981 cgttgaacag caggttgaac gaggtgtcgc tgaaatgcat caggtacgaa ccgaaccaca
     2041 cgccgctgtc gctggagcgg cgcggcgcaa cgttgtacga cagcgacagg ctgtgcccgc
     2101 gctgccacag gttgtcgtac gacagcgcgg cggtggcgcg cagcggcgtg gtcgactggc
     2161 tctggcggtt gttcagctcc aggctgccgt gcagcggcag cttgtcgtcc accaccaggt
     2221 cgacatccac cgtgccgggc gcattgccgg ccttgagcgc gggcgtgacg cggcggtcgg
     2281 cctgctggtt gagcgcggcc agatcgtgct gaacgcgggt gaagtccggc acctcgcctt
     2341 cggccagcga cggcgccagt tccttgatct ggtcgatcga gtggtacttc gagccgatca
     2401 cgttcaggtg ctcgatgtgc gactcgttga cctgcaggcg cacgatgcct tcgcgcaccg
     2461 actgcttggg gatgctgacc gacaccgtgc ggtagccgcg ctgcttgtag gcggcttcca
     2521 gcgatgcgcg cgcggcgtcg acatcgtctg ccgtgcgctc gggaccggtg tagcgcgaga
     2581 cggcgtcggt gatggtggct tcgtccagca cgctgttccc gtcgatgtcg aattcccaga
     2641 tgtcgatatg ccgcaccggc gccggtgccg gcgcggcgct caccacggcg caatggatga
     2701 gcagcgcaat gccagccgcc gcccacgcgc gcggattgcc tgccagttgc cgatccaaga
     2761 tttccccctg catacggcgc gcgccgtgtg aacggcagca cgatgcggcg caagccgccg
     2821 atgaatccgg caatcgaccg gtctgggatg ttaggcggcg ctacgttgca ggacgatgac
     2881 tgcgcgcgga ccaccgccgc attcactcct tatatggagg acgcattgca gtgcggtgat
     2941 gcaagcggtg cgttgatccg cgcaagcgga cgattgcctg cgcgggcttc aaccgcactg
     3001 ccgttttact tgttattatt tattatccaa tttacgcatt gtacattaat ttataacacg
     3061 tccactggca gtgcggttca ccgatttttt gcctactggg catcgtcatc ggagctttcc
     3121 ggcggtgctg gtgtgtcggg ctcgcagccg ggcggcagcg gcatctgcga acagtcgtcg
     3181 gactcggtgt cgttctcggg cgcacccatg ggaaccggcg gccgggtcgc gcttccggtg
     3241 gtcggcgcag tggccggaac gggcgccgtc ccggcatgca tcgcctgcgg ttgcggctgc
     3301 gcgcgcccgg gcggaaccgc gggcgcggct gcgaccggtg cggccgggct tgcggaagaa
     3361 tacgtgtgcg caggcgcgtc atcgccgtgc gagccgaaca gcgcatagac cagcaggccc
     3421 acgctggcca cgaagaagat ccagatgccg accgagcggt ccatcgatat gcgtctgcgg
     3481 aaaagatccg cgagcactat accgagcccg aagtcgcccg cagcgccgcg gtcgcagacg
     3541 gttcagccca gcagcgggcg gtagccggcg ggcacgccgc cgtcgcggat gatctgccgg
     3601 tacgcatcga ccgcggcagt gggcttgcgc tgcagccgcg ggtcggtcag cacgtccacc
     3661 gtgtacagac cgaagcgcgg gccgtacttg ccccactcga agttgtcggt gaggctccag
     3721 tggtagtagc cgcgcacgtc gacgtggttc tcgtcgcgcg cccgcgcgat cgcctcgagg
     3781 attcgcacca ccgcctcggc gcggcgctt
//
LOCUS       DASZZC010000449         4754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_41025,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000449 DASZZC010000000
VERSION     DASZZC010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41025"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VHE37_09475"
     CDS             complement(<1..266)
                     /locus_tag="VHE37_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35803.1"
                     /translation="MARTLYRDDQADARLARTSSLAQYLPHILLYPFTGFCLPVLILA
                     AVAVGIGVQSLFGISLIVIVGSWTLYYLISIVEHSAQGHATPPPL"
     gene            complement(268..678)
                     /gene="mscL"
                     /locus_tag="VHE37_09480"
     CDS             complement(268..678)
                     /gene="mscL"
                     /locus_tag="VHE37_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVT35804.1"
                     /translation="MGIMHEFRQFVMRGNVVDLAVGVVIGTAFGAIVNSLVGDIIMPV
                     IGILTSGVDFSKAAWVMREASADGKTPAVLLSYGKFLQTAINFLIIAFVIFLVVKGMN
                     TLKERYAAAPAPAPVSPQEKLLAEIRDLLKARQG"
     gene            862..1905
                     /locus_tag="VHE37_09485"
     CDS             862..1905
                     /locus_tag="VHE37_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT35805.1"
                     /translation="MSHRVTLADRSTGFDAEPQENLLAAGLRQRLALPFGCRSGTCAS
                     CRVRLLSGQVEYPVAPPALSAAEIDAGYILMCLAQPRSDLSIELHQPPQLDAIRPRTL
                     PCRVQQQRMLAHDVIGLILKLPKSNDSSSAFRFLPGQYIDFLLDGGRRRSFSIANAPA
                     PDLSGEVLELHLRVTPQGRFARWVLEEMPARAILQFEGPLGAFYVREDSARPLLFVAG
                     GTGFAPIKAMIERQLALGSARPMHLYWGARSRRDLYLHDLAAEWAAQHARLRYTPVLS
                     EPDEGWTGERGMVHEAVLRAQPDLRGYEAYLSGPPVMVRTGKLAFAAAGLDSDHLYYD
                     SFDYAYETWPVAG"
     gene            2088..2528
                     /locus_tag="VHE37_09490"
     CDS             2088..2528
                     /locus_tag="VHE37_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HVT35806.1"
                     /translation="MKTYLSKQIQKGFTLIELMIVVVIIGILAAIAIPAYQSYTARAE
                     GVSALTTLASLKPAMVYYLNKQETGTVDLNSLGLTTTPTLGTVTMSAWTNAVGGTNTL
                     TWAFASNANPINQGKTMTYTLTNASGTFSCTTTMDPSVKPKNCS"
     gene            2654..3304
                     /locus_tag="VHE37_09495"
     CDS             2654..3304
                     /locus_tag="VHE37_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT35807.1"
                     /translation="MAASRSSAEAKQNLSADDWAEAALEAMASGGIDAVAVEPLARRL
                     NVTKGSFYWHFDNREALLKSALDLWERNETDDVIRRVGVEPDPYQRIVKLFKHANSSY
                     QSGRLYLAIAAAEDHPVVSRAVRRVSQRRIDYLYGCYEALGFDEQQARHWARFAYATF
                     MGNLQISRDTPEEMPSGSQFNEYLRLMISTLIPRDGGGDAAGAAHPHVVRLRRQGN"
     gene            3324..>4754
                     /locus_tag="VHE37_09500"
     CDS             3324..>4754
                     /locus_tag="VHE37_09500"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVT35808.1"
                     /translation="MTTATLVVIAVAAFWVLAYLGAPLWIWTVAAAAGYIWLLGADAV
                     GAAGAVVLGTVVALLAFFCAVPLRRALVTRPFFALFKKILPEMSSTEREALEAGDVWW
                     EGDLFRGRPDWNKLLDFKYTQLTAQEQSFLDHECEELCRMVDDWKVEFEDKDLPPAVW
                     QYIKDKHFFAMLIKKEFGGLGFSAAAQSAVVTKLATKSIALAVTVMVPNSLGPGELLM
                     YYGTKEQQKQWLPGLIAGREIPCFGLTGPEVGSDATAMPDLGIVEYGQHEGKKVLGIR
                     LNFEKRYITLAPVATCVGLAFKLKDPQGLLGDKTRSDYGITCALVPANHPGIDIGRRH
                     YPGAAFMNGPIFGRDVFIPVDWIIGGPAMAGKGWRMLVECLSAGRGISLPALATAAGQ
                     SAYRMTGAYARIRRQFKVSIGKFEGVQEATGRIAAHAYTLEAMRILTASAVDQCAPSV
                     VTAIAKFHMTELMRRTVNDSMDVLSGR"
BASE COUNT          831 a         1539 c         1531 g          853 t
ORIGIN      
        1 aggggcggcg gcgtggcatg gccttgcgcg ctgtgctcaa cgatgctgat caggtaatac
       61 agcgtccagc tgccgacgat gacgatcagc gagatgccga acaggctctg cacgccgatg
      121 cccacggcca ccgcggccag gatcagcact ggcaggcaga agccggtgaa cgggtaaagc
      181 aggatgtgcg gcaggtactg cgccagcgac gaggtgcgcg cgagcctggc gtcggcttgg
      241 tcgtcgcggt acagcgtccg tgccacgcta gccctgtctg gccttcaaca ggtcgcggat
      301 ttcggcgagc agtttttcct gcgggctgac tggcgcgggc gccggtgcgg cggcgtagcg
      361 ctctttcagc gtgttcatcc ccttgaccac caggaagatg acgaaggcga tgatcaggaa
      421 gttgatcgcc gtctgcagga acttgccata ggagagcagc accgccggcg tcttgccgtc
      481 ggccgaggct tcccgcatca cccaggccgc cttggaaaaa tcgacgccgc tggtgaggat
      541 gccgatcacc ggcatgatga tgtcgccgac cagcgaattg acgatggcgc cgaacgcggt
      601 gccgatgacc acgccgacgg cgaggtcgac cacgttgccg cgcatcacga actgcctgaa
      661 ctcatgcatg atgcccatgc ttcgctcctg tgaatttccg acgggaaaaa cagctgaaaa
      721 accgctaacc gcacctgtca ttctactgtt ataaaatata atgtatatta taaataatac
      781 tatttttaat aacatgccag ttgcgagtgc ggtcggcctg ttcgagtgcg ccgcggcggc
      841 caggttcggt gctgccgccg catgagccat cgcgtcacgc tcgctgaccg ctccaccggc
      901 ttcgacgcgg agccgcagga aaacctgctg gccgccgggc tgcgccagcg gctcgccctg
      961 ccgttcggct gccgctccgg cacctgcgct tcgtgccgcg tgcgcctgct gtccggccag
     1021 gttgaatatc cggtggcgcc gccggcgctg tccgccgccg agatcgacgc gggctacatc
     1081 ctgatgtgtc tggcgcagcc gcgcagcgat ctgtcaatcg aactgcacca gccgccgcag
     1141 ctcgatgcga tccgtccgcg cacgttgcca tgccgcgtgc agcagcagcg catgctcgcg
     1201 catgacgtga tcgggctgat cctcaagctg ccgaaatcga acgacagcag cagcgcgttc
     1261 agatttcttc cggggcagta catcgacttc ctgcttgacg gcgggcgccg ccgcagtttc
     1321 tccatcgcca acgcgcccgc gccggacctg tccggcgagg tgctcgagct gcacctgcgc
     1381 gtcacgccgc agggccgctt cgcgcgctgg gtgctggagg aaatgcccgc gcgcgccatc
     1441 ctgcagttcg aaggaccact gggcgcgttc tacgtgcgcg aggattcggc acggccgctg
     1501 ctgtttgttg ccggcggcac cggatttgcg ccgatcaagg cgatgatcga gcggcagctg
     1561 gcgctgggca gtgcgcggcc gatgcacctg tactggggtg cgcgttcgcg gcgcgacctt
     1621 tacctgcacg atcttgcggc ggaatgggct gcgcagcacg cgaggctgcg ttacacgccg
     1681 gtgctgtccg agccggacga aggctggacc ggcgaacgcg gcatggtgca cgaggcggtg
     1741 ttgagggcac agcccgatct gcgcggctac gaggcctacc tgtccgggcc gccggttatg
     1801 gtgcgcaccg gcaaactggc attcgccgcg gccgggctgg attccgatca cctttattac
     1861 gattcgttcg actacgccta cgagacctgg ccggtggccg gttgaagcgg ccggcacctg
     1921 ccacgtttgg tcactcgctg acaaattatt cttccgcatg cgtcaaccgg cgagcgctgg
     1981 atctttttaa ctgcctgttt ttgcatgtcg tcgtccgctg atttgcttgg cacgggggat
     2041 gctgtactct gatggtgcgc gttaaatcca ctagggggca tttcgctatg aagacgtacc
     2101 tgagcaagca gatccagaag ggcttcaccc tgatcgaact gatgatcgtc gtggtcatca
     2161 tcggcattct ggctgccatt gccattccgg cctaccagtc ctacacggcc cgtgccgaag
     2221 gcgtttcggc gctgacgacg ctggcctcgc tcaagccggc catggtgtat tacctgaaca
     2281 agcaggaaac cggcacggtt gacctgaaca gcctgggcct gaccaccact cctacactgg
     2341 gaacggtgac catgtcggca tggaccaatg ccgtgggcgg caccaacacc ctgacctggg
     2401 cgttcgcttc gaatgccaac ccgatcaacc agggcaagac catgacctat accctgacca
     2461 atgcgagcgg cacgttctcc tgcaccacga cgatggaccc gagcgtcaag ccgaagaact
     2521 gctcctgatc tgaagcagcg tttcaacaac cggacggagc gtggggctcc gtccggtttt
     2581 ttattgcccg cgcttgacca tacgctaccg tacggcctag actgcggcgc ccttctcaag
     2641 tgcctgcact gatatggccg ccagccgcag cagcgccgaa gccaagcaga atctctccgc
     2701 cgatgactgg gccgaggccg cgctggaagc gatggccagc ggcggcatcg atgccgtggc
     2761 cgtcgagccg ctggcgcgca ggctcaacgt caccaagggc agcttctact ggcatttcga
     2821 caatcgcgag gcgctgctca agtcggcgct ggacctgtgg gagcgcaacg agaccgacga
     2881 tgtgatccgc cgggttggtg tcgagcctga tccgtaccag cgcatcgtca agctgttcaa
     2941 gcacgccaac agttcctacc agtccgggcg cctgtacctg gccattgccg cagccgagga
     3001 ccaccccgtg gtcagccgcg ccgtgcggcg cgtgtcgcag cgacgcatcg attacctgta
     3061 cggctgctat gaagccttgg gcttcgatga gcagcaggca cgccactggg cgcgcttcgc
     3121 ctacgccacc ttcatgggca acctgcagat ctcgcgcgac acgccggagg aaatgcccag
     3181 cggcagccag ttcaacgaat acctgcgcct gatgatcagt acgctgatcc cgcgcgacgg
     3241 cggcggcgac gcggcaggcg cggctcatcc gcacgtggtg aggctgcgcc gccagggcaa
     3301 ctgaggttcg cacggagctc gtcatgacca ccgccacact cgtcgtcatt gccgtggccg
     3361 cgttctgggt gctggcttac ctgggtgcgc cgctgtggat ctggaccgtg gctgcagcgg
     3421 ccggctacat ctggctgctg ggcgccgatg cggtcggagc cgctggtgcc gtcgtgctgg
     3481 gtacggtggt tgcgctgctc gcatttttct gtgcggtgcc gctgcggcgc gcgctggtga
     3541 cgcgtccgtt cttcgcgctg ttcaagaaga tcttgcccga gatgtcctcc accgagcgcg
     3601 aggccctgga agccggcgat gtctggtggg aaggcgacct gttccgcggc cgcccggact
     3661 ggaacaagct gctcgacttc aagtacacgc agctcaccgc gcaggagcag tcgttcctcg
     3721 accacgagtg cgaagaactg tgccgcatgg tcgacgactg gaaggtggag ttcgaggaca
     3781 aggacctgcc gccagcggtg tggcagtaca tcaaggacaa gcacttcttt gccatgctga
     3841 tcaagaagga gttcggcggg ctgggctttt ccgcggcggc gcaatccgcg gtggtgacca
     3901 agctcgccac caagtcgatc gcgctcgccg tgacggtgat ggtgcccaac tcgctggggc
     3961 cgggcgaact gctgatgtac tacggcacca aggaacagca gaagcaatgg ctgcccggcc
     4021 tgatcgcggg cagggagatt ccctgcttcg gcctcaccgg gccggaagtg ggatcggacg
     4081 ccaccgccat gcccgacctg ggcatcgtcg agtatggcca gcacgaagga aaaaaagtgc
     4141 tcggcatccg gctcaatttc gaaaagcgct acatcacgct ggcgccggtg gccacctgcg
     4201 tcggcctcgc cttcaagctg aaggatccgc agggactgct gggcgacaag acgcgcagcg
     4261 actacggcat cacctgcgcg ctggtgccgg cgaatcatcc gggcatcgac atcggccgcc
     4321 gccactaccc cggcgccgct ttcatgaacg gcccgatctt cggccgtgac gtgttcatcc
     4381 cggtggactg gatcatcggc ggcccggcca tggcaggcaa gggctggcgc atgctggtcg
     4441 agtgcctgtc cgccggccgc ggcatctcgc tgccggcgct ggccaccgcc gccggccaga
     4501 gcgcctatcg catgaccggc gcctatgcgc gcatccgccg ccagttcaag gtgtcgatcg
     4561 gcaagttcga gggcgtgcag gaagccaccg gccgcatcgc cgcccacgcc tacacgctgg
     4621 aagcaatgcg catccttacc gcctcggcag tggatcaatg cgcaccgtcg gtggtcaccg
     4681 ccatcgccaa gttccacatg accgagctga tgcgcaggac ggtgaacgac agcatggacg
     4741 tgctgtccgg ccgc
//
LOCUS       DASZZC010000450         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_864910,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000450 DASZZC010000000
VERSION     DASZZC010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864910"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..411)
                     /locus_tag="VHE37_09505"
     CDS             complement(133..411)
                     /locus_tag="VHE37_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35809.1"
                     /translation="MSRIQQALHNDYQAISLLRDELKLQAHLFRADARQRWQELEVSF
                     DTLKEHVQRAKTAAAVARPEVETASRLLADTLKAGYADIRNALKREVA"
     gene            complement(489..1889)
                     /locus_tag="VHE37_09510"
     CDS             complement(489..1889)
                     /locus_tag="VHE37_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HVT35810.1"
                     /translation="MSALLHWLQPYWLALYAAAALAAAMHALLHKRDPRSAWAWIAMC
                     WLFPLGGAALYWLIGINRVEGYARLGSARRRRGGWRGHSRSLRELEHAGASAGGNPLL
                     PGNSVELLVNGEQAYPAMLEAIAAARESIYLATYIYCDDDTGRRFAQALAQAHERGVR
                     VRVLLDGVSDFFYRPRASRLLAQLRVPYARFLPLRAWPPMLHVNLRNHRKLLVVDGRV
                     AFTGGMNVAEYHLLKSRAQAPVADLQFRIRGPLVQQLEDVFRADWQFASGEALAPSPA
                     PAADDAPGTAACRVITDGPENERDPFMLVLIAALAAAHRRVCIMTPYFLPPAPVAAAL
                     EATALRGIEVRIVLPLRSDQPWIDWATRHALAPLLRHGVQLYFGPPPFAHTKLLLVDD
                     QYVQFGSANLDVRSLRLNFELNVECYDAALSAQLHRHFDAHVRGARCVRVAQLTARPL
                     PARLRDAFFWLFSPYL"
     gene            complement(1886..>2243)
                     /locus_tag="VHE37_09515"
     CDS             complement(1886..>2243)
                     /locus_tag="VHE37_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVT35811.1"
                     /translation="LAPEHGSLDLVIAHMSLGRGTQQRQLDYVGRLLGGGAPAVLMGD
                     FNCDAELLRGHPELRRAGLLTPAHSPPTFPSWQPRRAIDHILVGGGVELHSLQALPHR
                     LSDHLPLAAEISLATP"
BASE COUNT          341 a          799 c          802 g          301 t
ORIGIN      
        1 gggcgccagg cacgcggcct cgaccagttc ctgcaacacg cccagcggca ccgcgtcggg
       61 caggtactcg cgtaccgcgc ggcgctcggc gagcgcctgc atcagcttca tttgcgtact
      121 ccgcggcggc gctcaggcca cttcgcgctt gagcgcgttg cggatgtcgg cgtagccggc
      181 cttcagcgta tcggccagca gcctcgacgc ggtctccact tcgggacgcg cgacggcggc
      241 cgccgtcttg gcgcgctgca cgtgctcctt cagcgtgtcg aagctcactt ccagttcctg
      301 ccagcgctgg cgcgcatcgg cgcggaacag gtgggcctgc agcttgagct cgtcgcgcag
      361 cagcgagatg gcctggtaat cgttgtgcaa ggcctgttgg atgcgggaca tgttggaatc
      421 tccggttggt gatgtgggcg gattgtggaa gcgcccggca aggtgcgcgt tgacctgagt
      481 caatcgcgct acaggtaggg cgagaacagc cagaaaaagg catcgcgcag gcgcgccggc
      541 agcggccgcg cggtcagctg cgccacgcgc acgcaccgcg cgccgcgcac gtgcgcatcg
      601 aaatgccggt gcagctgcgc cgacagcgcg gcgtcgtagc actccacgtt cagctcgaag
      661 ttgaggcgca ggctgcgcac gtcaagattg gccgagccga actgcacgta ctggtcgtcg
      721 accagcagca gcttggtgtg cgcaaacggc ggcggaccga agtacagctg cacgccgtgg
      781 cgcagcagcg gcgccagcgc atggcgcgtg gcccagtcga tccacggctg gtcgctgcgc
      841 agcggcagca cgatgcgcac ctcgatgccg cgcagcgcgg tggcctccag cgccgccgcc
      901 accggcgccg gcggcaggaa gtacggcgtc atgatgcaca cgcgccggtg cgccgcggcc
      961 agcgcggcga tcagcaccag catgaacgga tcgcgttcgt tctccggccc gtcggtgatc
     1021 acgcggcagg ccgccgtgcc cggcgcatcg tccgccgcag gtgccggcga aggcgccagc
     1081 gcctcgccgc tggcgaactg ccagtcggcg cggaacacgt cctccagctg ctgcaccagc
     1141 gggccacgga tgcggaactg caggtcggcc accggcgcct gcgcgcgcga cttgagcagg
     1201 tggtactcgg ccacgttcat gccgccggtg aaggcgacgc ggccatccac caccagcagc
     1261 ttgcggtggt tgcgcaggtt cacgtgcagc atcggcggcc acgcgcgcag cggcaggaag
     1321 cgcgcgtatg gaacgcgcag ttgcgccagc agccggctgg cgcgcgggcg gtagaagaaa
     1381 tcggacacgc catcgagcag cacgcgcacg cgcacaccac gttcatgcgc ctgcgccagc
     1441 gcctgggcga agcggcggcc ggtgtcgtcg tcgcaataga tgtacgtggc caggtagatc
     1501 gactcgcgcg ccgcggcgat cgcctcgagc atcgccgggt acgcctgctc accgttgacc
     1561 agcagttcca cgctgttgcc cggcagcagc gggttgccgc ccgcactggc gccggcgtgc
     1621 tccagttcgc gcagcgagcg cgagtggccg cgccagccgc cgcgccggcg ccgcgcgctg
     1681 cccagccgcg cgtagccctc cacacggttg atgccgatca gccagtacag cgcggcgcca
     1741 cccagcggga acagccagca catcgcgatc caggcccatg ccgagcgcgg gtcgcgcttg
     1801 tgcagcaggg cgtgcatcgc cgcagccagt gccgccgccg cgtacagcgc cagccagtac
     1861 ggctgcagcc agtgcagcag cgcgctcatg gcgtggccag cgaaatctcg gccgccagcg
     1921 gcaggtggtc ggacagcctg tgcggcagcg cctgcaggct gtgcagttcc acgccgccgc
     1981 cgaccaggat gtgatcgatg gcgcggcgcg gctgccagct gggaaaggtc ggcgggctgt
     2041 gcgccggcgt cagcagcccc gcgcggcgca gttccgggtg cccgcgcagc agctcggcat
     2101 cgcagttgaa atcgcccatc agcacggccg gcgcgccgcc gcccagcagc cggccgacat
     2161 agtccagctg ccgttgctgg gtgccccgcc ccagcgacat gtgcgcgatc accaggtcga
     2221 gcgagccgtg ctccggcgcc agg
//
LOCUS       DASZZC010000451         5043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_553193,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000451 DASZZC010000000
VERSION     DASZZC010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553193"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..887
                     /locus_tag="VHE37_09520"
     CDS             174..887
                     /locus_tag="VHE37_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HVT35812.1"
                     /translation="MSKLCVRSFAVSLDGYGAGPRQSLQNPLGERGPELMEWFFATDL
                     WKRMHQLGEGERGVDHRMAERGFDNIGAWILGRNMFGPVRGPWPDDSWKGWWGDEPPY
                     HVPVFVLTHHARAPLAMQGGTTFYFVTDGIESALAQARAAAGGRDVRLGGGVATVRQY
                     LQARLVDELHLVSRPVLLGAGEHLFAGLDLHALGYHCAESVAGERATHLMLRRASAPA
                     GTSEDQKMHDQIVKLHTGR"
     gene            904..1941
                     /locus_tag="VHE37_09525"
     CDS             904..1941
                     /locus_tag="VHE37_09525"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVT35813.1"
                     /translation="MSEKLQRMLGIELPVIQAPMAGVQGSALAIAVSNAGGLGSLPCA
                     MLSEDDLRKELAVIRAQTARPVNLNFFCHTTPTPDAALEQAWRQRFAGYYAELGIDAA
                     APARGGRQPFSAGMAALVREFRPAVVSFHFGLPSNELLDAVRASGAKIFSSATTLEEA
                     LWLEQRGVDAVIAQGVEAGGHRGMFLTADIATQTGTAQLLGQIVRAVKVPVIAAGGIA
                     HANGVRQALALGAAGVQVGTAFLLCPEATTSAVHRAALKSAAGRDTALTNVFSGRPAR
                     GIVNRLMRELGPMSTQAPPFPLAASASAPLRALAEKRGSGDFSPMWAGTDVSGCAEVP
                     AAQLLRQLVAT"
     gene            complement(1949..2545)
                     /locus_tag="VHE37_09530"
     CDS             complement(1949..2545)
                     /locus_tag="VHE37_09530"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HVT35814.1"
                     /translation="MSKLHECKVEHLRPTQITVGMLEVHDKRQHLRELGHHQRREFLR
                     EHAIPAVLGPNEKLYLTDHHHLGRALVDENIEHGFFLIEADLSALTDHAAFWLEMNAR
                     QWAHPVDPQGRRHDCTAIPHHLAELVDDPYRSLAGYVRNAGGYCKTQAAFAEFQWADF
                     FRRHVTIGAGHAAFAAAVAQALALARSPLAQHLPGYGG"
     gene            complement(2545..3021)
                     /locus_tag="VHE37_09535"
     CDS             complement(2545..3021)
                     /locus_tag="VHE37_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVT35815.1"
                     /translation="MNPELPDVLLRLGLATLIGAAIGLNRDLHGKPAGLRTHALVALG
                     SALIVLACGHIAADFHAADAQSRAIQGIITGVGFIGAGVIVHKVDQSKVHGLTTAAAV
                     WVAALVGAACGTGAFMPVSIAALLLALVLIFGGRIERRVHGWLHPRTDGTSGPTDS"
     gene            3157..3516
                     /locus_tag="VHE37_09540"
     CDS             3157..3516
                     /locus_tag="VHE37_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35816.1"
                     /translation="MNPKSIVESVKDGAAEAKHKASMVAAHGQEVVKAGAQTLQAAKE
                     VVIGGGREAVQLAARTGEELKRTLKQGAAQVGERLARIAEPTRKEQAQARKSEIKEKK
                     QRKRAEAMDGGQAQAGV"
     gene            complement(3529..>5043)
                     /locus_tag="VHE37_09545"
     CDS             complement(3529..>5043)
                     /locus_tag="VHE37_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVT35817.1"
                     /translation="GIDIFRVFDSLNAVDNMRVAMDAVRDSGALCEGTICYTADLFDT
                     SRPKYNLAYYVKMAKELERAGAHILGIKDMAGVCRPRAARELITALKNEVGLPLHFHT
                     HDTSGISAASVLAAVEAGCDAVDGALDAMSGLTSQPNLSSIAAALAGSERAPEVNLEA
                     MKSLSFYWEGVRRYYAPFEAEMRAGTADVYTHEMPGGQYTNLREQARAMGLDHRWPEI
                     SRAYADVNKLFGDIVKVTPTSKVVGDMALYMVANDLTPEMVADPARDIAFPASVISLM
                     RGELGFPPDGFPAALQKKILRGAAPLSGRAGALLPPVDLEAERKAAGDALGYVMDDGD
                     LCSHLMYPKVYREYAAHRSRYGDMSKLPTPVFFYGLKEQQEASIDLEPGKTLVIRLQG
                     RTENPDAGEDKLFFELNGQPRLIRVPRPGAAAKELAKAAEGNPKQVGAPMPGMVVTVA
                     VKPGQRVEKGDALLSIEAMKMETQLRAEHAGTVKRVLVNSGAVVAARDLLVEFE"
BASE COUNT          793 a         1669 c         1770 g          811 t
ORIGIN      
        1 acccgcggcg tcgaaaatca ctggaccgca ccggctgttg gcgtgttatt aaatatctgt
       61 caaaaataaa aatagacaat tatttgtaac atcgaaaatg ccggtgcggt ggagcgcttt
      121 tttgccgtgc agcggccaca ctatggcctc gcatttcctt gatgagcgga gccatgtcga
      181 aattgtgcgt gcggagtttt gccgtctccc ttgacggcta tggcgccggg ccccggcaga
      241 gcctgcagaa cccgctgggc gagcgcgggc cggaactgat ggagtggttc tttgccaccg
      301 acctgtggaa gcgcatgcac cagctcggcg agggcgaacg cggcgtggat caccgcatgg
      361 ccgagcgcgg cttcgacaac atcggcgcgt ggatcctcgg ccgcaacatg ttcggcccgg
      421 tgcgcggccc gtggccggat gacagctgga agggctggtg gggcgacgag ccgccgtacc
      481 acgtgccggt gttcgtgctc acgcaccatg cgcgcgcgcc gctggcgatg cagggcggca
      541 ccacttttta cttcgtcacc gacggcatcg agtccgcgct ggcgcaggcg cgcgccgccg
      601 ccggcgggcg cgacgtgcgc ctgggtggcg gcgtggccac cgtgcgccag tacctgcagg
      661 cgcggctggt cgacgagctg cacctggtct cgcggccggt gctgctcggc gcgggcgaac
      721 atcttttcgc cgggctcgat ctgcatgcgc tcggctacca ctgcgcggaa tcggttgccg
      781 gcgagcgggc cacgcacctc atgctcagga gagcttccgc gccggcgggc acgagcgaag
      841 atcagaagat gcacgaccag atcgtgaaac tgcacaccgg ccgatgaagc cgcgcggcct
      901 gccatgagcg aaaaactgca acgcatgctg ggcatcgagc tgcccgtcat ccaggcgccg
      961 atggccggcg ttcagggcag cgcgctggca atcgcggtat cgaacgcggg cgggctcggc
     1021 tcgctgccct gcgcgatgtt gtcggaagac gatttgcgca aggagctcgc cgtcatccgc
     1081 gcgcagaccg cgcgcccggt caatctcaat ttcttctgcc acacgacacc aacgccggat
     1141 gccgcgctgg agcaggcatg gcgccagcgc tttgccggct actacgccga gttgggcatc
     1201 gatgccgccg cgcctgcgcg cggcggacgc cagccgttca gcgccgggat ggcggcgctg
     1261 gtgcgggaat tccgtccggc cgtggtcagc ttccatttcg gtcttccatc gaacgaattg
     1321 ctcgatgccg tgcgggccag cggcgcgaag attttctcat cggcgaccac gctcgaggaa
     1381 gcgctgtggc tggagcagcg cggagtcgat gcagtgatcg cgcagggcgt cgaggccggt
     1441 ggccatcgcg gcatgttcct caccgcggac atcgccacgc agaccggtac tgcgcagttg
     1501 cttgggcaga tcgtgcgcgc agtgaaagtg ccggtgatcg ctgccggcgg catcgcgcat
     1561 gccaatggcg tgcggcaggc gctggcgctg ggcgcggccg gcgtgcaggt cggcactgcg
     1621 ttcctgctgt gcccggaggc gaccaccagc gccgtgcacc gcgctgcact gaagagcgcc
     1681 gccgggcgcg ataccgcgct gaccaacgtg ttcagcggac gtccggcgcg cggcatcgtc
     1741 aaccgcctga tgcgcgaact ggggccgatg agcacgcagg cgccgccgtt tccgctcgcc
     1801 gcgtccgcct ccgcgccgct gcgcgcgttg gcggagaaac gcggcagcgg cgatttctcg
     1861 ccgatgtggg cgggcaccga cgtgtcgggc tgcgcggagg ttcccgccgc gcagttgctg
     1921 cgccagctgg tcgcaaccta gccgcaacct agccgccgta acccggcagg tgttgtgcaa
     1981 gcgggctgcg cgccagcgcg agcgcctggg ccaccgcggc tgcaaaggcg gcgtgcccgg
     2041 cgccgatggt cacgtgcctg cggaagaaat ccgcccactg gaattccgcg aacgccgcct
     2101 gcgtcttgca gtagccgccg gcgttgcgca cataaccggc caggctgcgg taggggtcgt
     2161 cgaccagttc cgccaggtga tgcggtatgg cggtgcagtc gtggcgccgc ccctgcgggt
     2221 ccaccgggtg cgcccactgg cgcgcgttca tctccagcca gaacgcggcg tggtcggtca
     2281 gcgcagacag gtcggcctcg atcaggaaga atccgtgctc gatgttctcg tccaccagcg
     2341 cgcggccgag gtggtgatga tcggtgaggt agagcttctc gttcggaccg agcaccgccg
     2401 ggatcgcatg ctcgcgcagg aattccctgc gctggtggtg gccgagttcg cgcaggtgct
     2461 ggcgcttgtc gtgcacctcg agcatgccga cggtgatctg cgtaggccga aggtgctcca
     2521 ccttgcactc gtgcagcttg ctcatcagct gtcggtgggc ccgctggtcc cgtcggtcct
     2581 ggggtgcagc cagccatgca cgcggcgctc gatgcgtccg ccgaagatca gcaccagcgc
     2641 cagcagcaac gcggcaatcg acaccggcat gaacgcgccg gtgccgcagg ccgcgccgac
     2701 tagcgccgcc acccacaccg ccgccgcggt ggtgagcccg tgcactttcg actggtcgac
     2761 cttgtgcacg atgacgccgg cgccgatgaa gccgacgccg gtgatgatcc cctggatggc
     2821 gcggctttgc gcatcggccg cgtggaagtc ggcggcgatg tgtccgcagg ccagcacgat
     2881 cagcgccgag cccagcgcca ccagcgcatg cgtgcgcaag cccgccggct tgccgtgcag
     2941 gtcgcggttg aggccgatgg ccgcgccgat caaggtggcc aggcccaggc gcagcagtac
     3001 gtcgggcagt tcagggttca tggcgcgagt gtaggcggat ttgccttgtt gcggactgca
     3061 cgatgggcgc cgagtcgccg aatggggaac tcggctgact tccgcaaagt cctgcttaag
     3121 agcgtctggt ttttcacaca tcagcaggag aacggcatga acccgaagtc catcgtcgaa
     3181 tcggtcaagg acggcgcggc agaggcgaaa cacaaggcca gcatggtggc agcacacggc
     3241 caggaggtgg tgaaggccgg cgcgcagacg ctgcaggcag ccaaggaagt ggtgatcggc
     3301 ggcgggcgcg aagcggtgca gctggccgcg cgcaccggcg aagagctgaa gcgcacgctg
     3361 aagcaaggcg ccgcacaggt gggcgaacgc ctggcgcgca tcgccgagcc cacgcgcaag
     3421 gaacaggcgc aggcgcgcaa gtcggaaatc aaggagaaga agcagcgcaa gcgcgccgaa
     3481 gcaatggacg gcgggcaggc gcaggcgggg gtctgatcgc gcgggcggct actcaaactc
     3541 caccagcaaa tccctcgccg ccaccaccgc ccccgaattc accagcacgc gcttgaccgt
     3601 gcccgcgtgc tccgcgcgca gctgcgtctc catcttcatc gcctcgatcg acagcagcgc
     3661 atcgcctttc tcgacgcgct ggccgggctt caccgccaca gtcaccacca tgccgggcat
     3721 cggcgcgccg acctgtttcg gattgccttc cgcggccttg gccagttcct tcgccgcggc
     3781 gccggggcgc ggcacgcgga tcaggcgcgg ctggccgttg agttcgaaga acagcttgtc
     3841 ctcgccggcg tcgggattct cggtgcggcc ctgcaggcgg atcaccagcg tcttgccggg
     3901 ttcgaggtcg atgctcgctt cctgctgttc cttcaggccg taaaagaaca ccggcgtcgg
     3961 cagcttgctc atgtcgccgt agcgcgagcg gtgcgcggcg tactcgcggt agaccttggg
     4021 atacatcagg tgcgagcaga ggtcgccgtc gtccatgacg tagccgagcg catcgccggc
     4081 ggccttgcgt tcggcttcca ggtccaccgg cggcagcagc gccccggcgc gcccggacag
     4141 cggcgccgcg ccgcgcagga ttttcttctg cagcgccgcg gggaatccat ccggtggaaa
     4201 gccgagctca ccacgcatca gcgagatgac cgatgcgggg aaggcgatgt cgcgcgccgg
     4261 gtcggccacc atctccggcg tcaggtcatt ggccaccatg tacagcgcca tgtcgccgac
     4321 caccttcgac gtgggcgtga ccttgacgat gtcgccgaat aatttgttca cgtcggcata
     4381 ggcgcggctg atctccggcc agcgatggtc gagtcccatg gcgcgcgcct gctcgcgcag
     4441 gttggtgtac tggccgccgg gcatctcgtg ggtgtagacg tcggcggtgc cggcgcgcat
     4501 ctcggcctcg aacggcgcgt agtagcggcg cacgccttcc cagtagaagg agagcgactt
     4561 catcgcctcg aggttcacct cgggcgcccg ctcgctgccg gccagcgcag cggcgatcga
     4621 cgacaggttg ggctgcgagg tgagcccgct catggcgtcg agcgcgccat ccaccgcgtc
     4681 gcagccggcc tcgaccgcgg cgagcacgct ggccgcgctg atgccgctgg tgtcatgcgt
     4741 gtggaagtgc agcggcaggc ccacttcgtt cttcagggca gtaatcagtt cgcgcgcggc
     4801 gcgcgggcgg cacacgccgg ccatgtcctt gatgccgaga atgtgcgcgc cggcgcgctc
     4861 cagttccttc gccatcttca cgtagtaggc gaggttgtac ttgggccgcg aggtgtcgaa
     4921 caggtccgcc gtgtagcaga tggtgccttc gcacagcgcg ccgctgtcgc gcaccgcgtc
     4981 catcgccacg cgcatgttgt cgacggcgtt gagcgagtcg aacacgcgga agatgtcgat
     5041 gcc
//
LOCUS       DASZZC010000452         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_488200,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000452 DASZZC010000000
VERSION     DASZZC010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488200"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VHE37_09550"
     CDS             complement(<1..480)
                     /locus_tag="VHE37_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35818.1"
                     /translation="MNNNNPRFSFFRAAALLLALAAAGCGHSVPGAIGKDSGGAAAAT
                     KTYTICPGADAQKNAIIAFFDAHEGDTIQFCEGQFDFTNSLVMTGKKGITVLGAGRKK
                     TFIRFAGSNDQDGFNFNRMTGITVQGLTIYDAPGNGLRFFRSDYVTVRDVKVGWTVAD
                     "
     gene            774..>2211
                     /locus_tag="VHE37_09555"
     CDS             774..>2211
                     /locus_tag="VHE37_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVT35819.1"
                     /translation="MRLRHSCAALICALLPVACGHSLPGAVDQSGVVASGGAKFDATV
                     TRTEMGIPHILANDFGSLGYGYGYAFAEDNLCVLQEDLVTIRGERSKYMGASGSYTIQ
                     PIGVTADNITSDFFWKFTATDAAIAPLKANTDPDYQKVTSGFVAGYNRYIAELKAGAH
                     PGRHAACADQPWLFPITDDDMYRRYFRLALIASSSVFINEIANAAPSLTGASPGTGSG
                     TSAAPAPTQQQIVDALRAQPNALTALHDHGRFGSNMYGLGKDSTNTGQPLLLGNPHFP
                     WIGTERLYIAHATLPGKIDIMGGALYGVPAINIGFNDHFAWSHTVSTAFRFTLYQLTL
                     NPADPTQYLYNGQFVPMTATDVVIDVKGTDGSITQQHRTLYRSQYGPMLVLTASGVPV
                     LGWNRALAYTLRDANAENDRLINQFARWNTATSLAEFIDAHKSVLGIPWVNTVATGPD
                     QQVYYGDVSVVPNVPDSKVTSCAAHPIHD"
BASE COUNT          401 a          762 c          669 g          379 t
ORIGIN      
        1 gtcggccacg gtccagccga ccttcacgtc gcgcacggtc acgtaatcgg agcggaagaa
       61 gcgcaggccg ttgcccggcg cgtcgtagat ggtcaggccc tgcaccgtga tgccggtcat
      121 gcggttgaag ttgaacccgt cctggtcatt gctgccggca aaacggatga aggtcttctt
      181 gcgcccggcg ccgagcacgg tgatgccctt cttgccggtc atcaccagcg agttggtgaa
      241 atcaaactgg ccttcgcaga actggatggt gtcgccttcg tgggcatcga agaaagcgat
      301 gatggcgttc ttctgcgcgt cggcaccggg gcagatggtg taggtcttgg tggccgcggc
      361 cgcgccgccg ctgtccttgc cgatggcgcc gggcaccgag tggccgcagc ccgcagcggc
      421 cagcgccagc agcagcgcag ccgcgcggaa aaaagaaaag cggggattgt tgttgttcat
      481 gggaagttcc gcgcggcgtt gcgctcgttg caggacggtg acaatagttt ttcaacccgc
      541 gaaagaccag tggtaaaagt tcgcaggcag gattttctgc cccaccgcac tgcccttggg
      601 catgttataa aaaatctatt aattttgtaa ttaatgtatt ttttataaca ccatggaacc
      661 gggtgcggtg gaccttctgc gcgcgcctgc gtcggcgccg gcgctatagt gcggcccacc
      721 tgacggccgc accgatggcc gcgtcatcat ccaccgcggg gaggagatca cccatgcgtt
      781 tgcgtcactc gtgtgccgcg ctgatttgcg cgctgttgcc cgttgcctgc ggtcactcgc
      841 tgccgggcgc cgtcgaccag agcggcgttg tcgccagcgg cggtgccaag ttcgacgcca
      901 ccgtcacgcg caccgagatg ggcatcccgc acatcctggc caacgatttc ggctcgctgg
      961 gctacggcta cggctacgcg ttcgccgagg acaacctctg cgtgctgcag gaagacctgg
     1021 tcaccatccg cggcgaacgc agcaagtaca tgggcgccag cggcagctac accatccagc
     1081 ccatcggcgt caccgccgac aacatcacca gcgacttctt ctggaagttc accgccaccg
     1141 atgccgccat cgcgccgctg aaggcgaaca ccgatccgga ctaccagaaa gtcaccagcg
     1201 gcttcgtcgc cggctacaac cgctacatcg cagagctgaa agccggcgca catcccggcc
     1261 gccacgcggc ctgcgccgac cagccctggc tgttcccgat caccgacgat gacatgtacc
     1321 gccgctattt ccgcctggcg ctgattgcct cgtcgtcggt gttcatcaac gagattgcca
     1381 atgcggcgcc gtcgctcacc ggcgccagcc ccggcaccgg ctcgggcacc tcggctgcgc
     1441 cggcgccgac gcagcagcag atcgtcgacg cgctgcgcgc gcagcccaac gcgctcaccg
     1501 cgctgcacga tcacggccgc ttcggcagca acatgtacgg cctgggcaag gattccacca
     1561 ataccgggca gccactgctg ctcggcaacc cgcactttcc gtggatcggc accgagcgcc
     1621 tgtacatcgc gcacgccacg ctgccgggca agatcgacat catgggcggc gcgctctacg
     1681 gcgtgccggc gatcaacatc ggcttcaacg atcatttcgc gtggagccac accgtctcca
     1741 ccgcgttccg cttcacgctc taccagctca cgctcaaccc ggccgatccg acgcaatacc
     1801 tctacaacgg ccagttcgtg ccgatgaccg ccaccgatgt ggtgatcgac gtcaagggca
     1861 ccgacggcag catcacccag cagcaccgca cgctgtatcg cagccagtac ggcccgatgc
     1921 tggtgctcac cgccagcggc gtgccggtgc tgggctggaa ccgcgcgctg gcctacacgc
     1981 ttcgcgacgc caacgcggag aacgaccggc tgatcaatca atttgcgcgc tggaacacgg
     2041 ccacctcgct ggccgagttc atcgacgcgc acaagagcgt gctcggcatt ccatgggtga
     2101 acaccgtggc caccggcccg gaccagcagg tgtattacgg cgacgtgtcc gtggtgccca
     2161 acgtgcccga cagcaaggtc acctcctgcg cggcgcaccc gatccacgat g
//
LOCUS       DASZZC010000453         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_292849,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000453 DASZZC010000000
VERSION     DASZZC010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292849"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VHE37_09560"
     CDS             complement(<1..1068)
                     /locus_tag="VHE37_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVT35820.1"
                     /translation="MSTVIENERQPLRVFAEKAYLDYSMYVVLDRALPHVADGLKPVQ
                     RRIIYAMSELGLSAQSKHKKSARTVGDVIGKFHPHGDSACYEAMVTMAQPFSYRYPLV
                     DGQGNWGSPDDPKSFAAMRYTESRLTPYAAVLLAELDQGTSDWVPNFDGTLEEPKLLP
                     ARLPNILVNGTTGIAVGMATDIPPHNLRELASACLHLLKHPNAHVETLCEHVPGPDYP
                     TGCEVVSEPHELLKVYQTGNGQIRARARWEKDDDTGNIVITALPHQVSGARVQEQIGE
                     QMRNKKLPLLDDIRDESDHENPTRIVLIPRSNRVDADQLMAHLFATTDLEKSYRVNLN
                     VIGLDGRPKVKNLKELLSEWLD"
     gene            complement(1065..2957)
                     /gene="parE"
                     /locus_tag="VHE37_09565"
     CDS             complement(1065..2957)
                     /gene="parE"
                     /locus_tag="VHE37_09565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HVT35821.1"
                     /translation="MAASNYSAESIEVLEGLDPVRKRPGMYTDTQRPNHLAQEVIDNS
                     VDEALQGHARNIGVTVFEDGSLQVVDDGRGMPVDINPKFGVTGVELILTKLHSGAKFS
                     GKEYGFSGGLHGVGVSVVNALSSKLEVRVCRGGIEYQMDFKDGNRASKLKEIGTVGKN
                     RTGTTVRFWPDAKYFDTAKFAIPRLKQVLRAKAVLCPNLRVTLDDQVNNEKCEWQYEN
                     GLPDYLKDALKGAETIPAEPFVGKFAAAREEVEWALVWLNSDAEAVAESYVNLIPTIQ
                     GGTHVNGLRTGLTEAIREFCEFRNLLPRGVKLTPEDAWQGCCYVLSTKMHDPQFAGQT
                     KERLSSRESATFVGGVVHDAFSLWLNQHPEQGEQVAALVIANATARMKQAKQVVRKKI
                     TSGPALPGKLADCVSTDLSQTELFLVEGDSAGGSAKQARDRDTQAVMPLRGKILNAWE
                     CDANEALASNEIHDISVAIGVDPGSADIAGLRYGKICVLADADSDGLHIATLLCALFL
                     KHFRPLVEAGHIYIACPPLFRIDAGKQVFYALDEGEKQGVLDRLSAEGNKSKLSVTRF
                     KGLGEMNPLQLRETTMARDTRRLVQLTLDGDSPDEMMDKLLAKKRAGDRKEWLEENGN
                     KAGIEV"
     gene            3107..3361
                     /locus_tag="VHE37_09570"
     CDS             3107..3361
                     /locus_tag="VHE37_09570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HVT35822.1"
                     /translation="MKKTDNHDPLARFEDAMKELEAIVQKMERGDLRLEESLKLFERG
                     MELTAQCRTSLDTAELKVKNLLERHTPPAPEAAADADDDA"
     gene            3358..>4220
                     /locus_tag="VHE37_09575"
     CDS             3358..>4220
                     /locus_tag="VHE37_09575"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HVT35823.1"
                     /translation="MNAAFATRVEQYQQRLDAVMSRWLPAATAHPARLHQAMRYACEG
                     GKHLRPQLVYAAGEVLGVDARELDGAAAAVELIHAYSLVHDDLPAMDNDDLRRGKPTC
                     HRAFDEATAVLAGDALQALAFQLLAEDRESQLQPQQRVAMIATLAEAAGSHGMVGGQA
                     IDLESEGRDLNLAELESLHVKKTGALIRACVRLAAHARVALDHAQREALDRYGKCIGL
                     AFQIQDDVLDVESNSAKLGKTAGKDEAALKSTYPSVMGLPAAKQRAADLFGQARAALA
                     VFGDTAEPLHWL"
BASE COUNT          682 a         1392 c         1373 g          773 t
ORIGIN      
        1 atcgagccac tcggacaaca gctccttgag gttcttcacc ttaggccggc cgtcgaggcc
       61 gatgacgttg agattgacgc ggtagctctt ctcgagatcg gtggtggcga acaggtgcgc
      121 catcaactgg tcggcgtcca cgcggttgga gcgcgggatc agcacaatgc gcgtcgggtt
      181 ctcgtggtcc gactcgtcgc ggatgtcgtc gagcagcggc agcttcttgt tgcgcatctg
      241 ctcgccgatc tgttcctgca cgcgcgcgcc ggatacctga tgcggcagag cggtgatgac
      301 gatgttgccg gtgtcgtcgt ccttttccca gcgcgcgcgg gcgcggatct ggccattgcc
      361 agtctggtac accttcagca gttcatgcgg ctcgctgacg acctcgcagc cggtcggata
      421 gtccgggccc ggcacgtgct cgcacagggt ttccacgtgc gcattcggat gcttgagcag
      481 gtgcaggcag gcggacgcca gctcgcgcag gttgtgcggc gggatgtcgg tggccatgcc
      541 caccgcaatg cccgtggtgc cgttgaccag gatgttgggc aggcgcgcgg gcaggagctt
      601 gggctcttcc agcgtgccgt cgaagttggg cacccagtcg gacgtgccct ggtcgagttc
      661 cgccagcagc accgccgcgt acggcgtcag gcgcgactcg gtgtagcgca tcgcggcaaa
      721 ggatttcgga tcgtccggcg agccccagtt gccctgcccg tccaccagcg ggtagcggta
      781 ggagaacggc tgcgccatcg tcaccatcgc ctcgtagcag gcgctgtcgc cgtgcggatg
      841 gaacttgccg atgacgtcgc caacggtgcg cgccgacttc ttgtgcttgc tctgcgccga
      901 caggcccagc tcgctcatgg cgtaaatgat gcggcgctgc accggtttca aaccgtcggc
      961 cacgtgcggc agcgcgcggt ccagcaccac gtacatcgaa taatccaggt acgccttctc
     1021 ggcgaagacg cgcagcggtt gacgctcgtt ttcgatgaca gtgctcatac ttcaatcccg
     1081 gccttgttgc cgttttcttc cagccattcc ttgcggtcgc cagcgcgctt cttcgccagc
     1141 agcttgtcca tcatttcgtc gggcgaatcg ccgtcgagcg tcagctgcac cagccggcgc
     1201 gtgtcgcgcg ccatggtggt ttcgcgcagc tgcagcgggt tcatctcgcc caggcccttg
     1261 aagcgcgtga ccgacagctt cgacttgttg ccttccgcgc tcaggcggtc gagcacgccc
     1321 tgcttctcgc cttcatccag cgcgtagaac acctgcttgc cggcgtcgat gcggaacagc
     1381 ggcgggcagg cgatgtagat atggccggct tcgaccagcg ggcggaaatg cttgaggaac
     1441 agcgcgcaca gcagcgtggc gatatgcaga ccgtcgctgt cagcgtcagc cagcacgcag
     1501 atttttccat agcgcaggcc ggcgatgtcg gcgctgccgg gatcgacgcc gatggccacg
     1561 ctgatgtcat ggatttcgtt cgaggccagc gcctcgttgg cgtcgcattc ccaggcgttc
     1621 aaaatttttc cgcgcaaagg catcaccgcc tgcgtgtcgc ggtcgcgcgc ctgcttggcg
     1681 ctgccaccgg cggagtcgcc ttccaccagg aacagctcgg tctgcgacag gtcggtggat
     1741 acgcaatcgg ccagcttgcc gggcagcgcc gggccgctgg tgattttctt gcgcaccacc
     1801 tgcttggcct gcttcatgcg cgcggtggcg ttggcgatga ccagcgcggc cacctgctcg
     1861 ccctgctccg ggtgctggtt cagccacagc gagaaggcgt cgtgcaccac gccgccgacg
     1921 aaggtcgccg actcgcgcga gctcaatcgc tccttggtct gcccggcaaa ctgcggatcg
     1981 tgcatcttgg tggacagcac gtagcagcag ccctgccagg catcttccgg cgtcagcttc
     2041 acgccgcgcg gcagcaggtt gcggaactcg cagaactcgc ggatcgcctc ggtcaggccg
     2101 gtgcgcaagc cgttgacgtg cgtgccgccc tggatggtcg gaatcaggtt cacgtacgac
     2161 tcggcgaccg cttctgcgtc gctgttgagc cacaccagcg cccattccac ttcctcgcgc
     2221 gcggcggcga acttgccgac gaagggttcg gccggaatgg tctcggcgcc cttaagcgcg
     2281 tccttcaggt aatcaggcag gccgttctcg tactgccatt cgcacttctc gttgttgacc
     2341 tggtcgtcga gcgtgacgcg caggttcggg cacagcacgg ccttggcgcg cagcacctgc
     2401 ttcaagcgcg gaatggcgaa tttggccgtg tcgaaatatt tcgcgtcggg ccagaagcgc
     2461 acggtggtgc cggtgcggtt cttgccgacg gtgccgatct ccttcagctt ggacgcgcgg
     2521 ttgccgtcct tgaagtccat ctggtactcg atgccgccgc ggcacacgcg cacttcgagc
     2581 ttgctcgaca gcgcgttgac taccgacacg cccacaccgt gcaggccgcc ggaaaatccg
     2641 tattccttgc cggagaactt ggcgcccgag tgcagcttgg tgaggatgag ctcgacaccg
     2701 gtgacgccga atttcggatt gatatccacc ggcatgccgc ggccgtcgtc caccacctgc
     2761 agcgagccgt cttcgaacac cgtcacgccg atgttgcgcg cgtggccctg cagcgcctcg
     2821 tccaccgaat tgtcgatgac ttcctgcgcc aggtggttgg gccgctgcgt gtcggtgtac
     2881 atgcccgggc gcttgcgcac cgggtcgagt ccctcgagga cttcgatcga ttctgcggaa
     2941 taattgctgg ctgccatctc gtgttccgtt gggcgccggg aggcggcccg cgttaaaata
     3001 tttggcgccg gtacgtccac cgcaccggcc ggtagcgtgt tacataagct actaattatt
     3061 aaacaataga ttacattcta tcacagcctc agaggcggtg cggtccatga aaaaaaccga
     3121 caaccacgat ccgctcgccc gcttcgaaga cgccatgaaa gagctcgaag ccatcgtgca
     3181 gaagatggag cgtggcgacc tccggctgga ggaatcgctc aagctgttcg agcggggcat
     3241 ggagcttacc gcgcaatgcc ggacttcgct ggacaccgcc gaactcaagg tgaaaaacct
     3301 gctggagcgc cacacaccgc ccgcgccgga agcggccgct gacgcggacg acgacgcttg
     3361 aacgccgcct tcgccacccg cgtcgagcag tatcagcaaa ggctcgacgc ggtgatgtcg
     3421 cgctggttgc ccgcggccac cgcgcacccc gcgcgcctgc accaggccat gcgctacgcc
     3481 tgcgagggcg gcaagcacct gcgcccacag ctggtctacg ccgccggcga agtgctcggc
     3541 gtcgatgcgc gcgagctcga cggtgccgcc gcagccgtcg agctgatcca cgcctactcg
     3601 ctggtgcacg acgatctgcc ggcgatggac aacgatgatt tgcgccgcgg taaaccgacc
     3661 tgccaccgcg ccttcgacga ggccaccgcg gtgctggcgg gggatgcgct gcaggcgctg
     3721 gcttttcagc tgctggcgga ggaccgcgaa tcacagctgc aaccgcagca gcgcgtggcg
     3781 atgatcgcca cgctggccga agcggcaggc tcgcacggca tggtcggcgg ccaggccatc
     3841 gatctcgagt ccgaagggcg cgatctcaac ctggccgaac tggaatcgct gcacgtgaaa
     3901 aagaccggcg cgctgatccg cgcctgcgtg cgcctggcgg cgcatgcgcg cgtcgcgctc
     3961 gaccacgccc agcgcgaagc gctggaccgc tacggcaagt gcatcgggct ggccttccaa
     4021 atccaggacg acgtgctcga cgtggagagc aacagcgcca agctgggcaa gaccgcgggc
     4081 aaggatgagg ccgcgctcaa gtcgacctac ccgtcggtga tgggcctgcc cgccgccaag
     4141 cagcgcgcgg ccgacctgtt cgggcaggcg cgcgccgcgc tcgcggtctt cggcgatacg
     4201 gctgaaccct tgcactggct
//
LOCUS       DASZZC010000454         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_488375,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000454 DASZZC010000000
VERSION     DASZZC010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488375"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1401
                     /locus_tag="VHE37_09580"
     CDS             163..1401
                     /locus_tag="VHE37_09580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HVT35824.1"
                     /translation="MDITQFLLDRISRRFGPGSLEIEAPGGRRWQLGRGEPHARMVLH
                     RAPALARMLLDPELAFGESYVDGDWDAPDGLLPVLHAALSMTQPLREGPGSRRWRRLR
                     SLGGEFNSRARSRRNVEHHYDLDFDLYRRFLDADLHYSCAYFREPGMSLEAAQQAKCA
                     HIAAKLDLAPGAQVLDIGCGWGSLALYLAEHFDVRVTGITLSREQLDVARRRALQRGL
                     AGRVDFRLQDYRETAGAYDAIVSVGMFEHVGRPYYDAFFRQLPQLARPDTVMLLHTIG
                     RSSPPGSTNPWVRKYIFPGGYTPAASEVLAAAEKSGLVATDLEVWRLHYASTLREWHV
                     RFQQARADIRERMGERFCRMWEFYLQACEASFRWDDLRVFHLQLSARQDRLPLTRDYL
                     YRAADVESREPARARLRSVR"
     gene            complement(1398..>2066)
                     /locus_tag="VHE37_09585"
     CDS             complement(1398..>2066)
                     /locus_tag="VHE37_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT35825.1"
                     /translation="DAHRWCLGLRSALERRGVKFTFGCEVRRIVAGRGAVRGVETSAG
                     LIDAAHIVLAAAAWSPQLAAPLGIKLAIRPAKGYSLSVPMRGAARMPLMAIIDDSQHT
                     SATPLGAVLRLAGTAEFCGFDFSLRPERLHNLWRYLRALGPSLEADADRQRAVAWCGL
                     RPMAADGRPYIGATPVAGLWLNCGHGHLGWTQGAGSGRLLAQLISGAATAIDPGPYAL
                     ARGR"
BASE COUNT          302 a          739 c          720 g          305 t
ORIGIN      
        1 ccggttcgat cgactgcgcg gtcagccgcg cgaaggcttc gtcgtaatcg gctaccgcct
       61 gctgccagcc ggcgtgctcg agttcatgga aggtgctcat gccgccatgg tagcgctgct
      121 gccgcgccga acatgcgctg cgccggcgct gtcaaaagca ccatggacat cacgcaattc
      181 ctgctcgacc gcatcagccg gcgcttcggg cctggttcac tcgaaatcga ggcgccggga
      241 gggcgccgct ggcagctggg ccgcggcgag ccgcacgcgc gcatggtgct gcaccgcgcg
      301 ccggcgctgg cgcgcatgct gctggatccg gaactcgcct tcggcgaaag ctatgtcgac
      361 ggcgactggg acgcgcccga tggcctgctg ccggtgctgc acgccgcgct gtcgatgacg
      421 cagccgctgc gcgaagggcc cggcagccgg cgctggcggc gcctgcgtag cctgggcggc
      481 gagttcaact cgcgcgcgcg cagccggcgc aacgtggagc accactacga tctcgacttc
      541 gatctctacc gccgtttcct cgatgccgac ctgcattatt cctgcgcgta tttccgcgag
      601 cccggcatga gcctggaagc ggcgcagcag gccaagtgcg cgcacatcgc ggccaagctc
      661 gaccttgcgc ccggcgcgca ggtgctggac atcggatgcg gctggggcag cctggcgctg
      721 tacctggccg agcatttcga cgtgcgcgtc accggcatca cgctgtcgcg cgaacagctt
      781 gacgtcgcac gccgccgcgc gctgcagcgc gggctggctg gccgcgtcga cttccgcctg
      841 caggattacc gcgaaacggc gggcgcctac gacgccatcg tcagcgtcgg catgttcgag
      901 cacgtgggcc gtccgtacta cgacgccttc ttccgccagc tgccgcagct ggcgcggccc
      961 gacacggtga tgctgctgca caccatcggc cgcagttcgc cgcccggcag cacgaatcca
     1021 tgggtgcgca agtacatctt ccccggcggc tacacgccgg ccgcatcgga agtgctggcc
     1081 gcggcggaaa agagcggcct ggtcgccacc gatctcgagg tgtggcgcct gcattacgcc
     1141 agcacgctgc gcgagtggca cgtgcgcttc cagcaggcgc gcgccgacat ccgcgagcgc
     1201 atgggcgaac gcttctgccg catgtgggag ttctacctgc aggcctgcga agccagcttc
     1261 cgctgggacg acctgcgcgt gttccacctg cagctgagtg cgcggcagga ccgcctgccg
     1321 ctgacgcgcg attaccttta tcgcgctgcc gatgtggagt cgcgcgaacc ggcacgggcg
     1381 cgtctgcgca gcgtgcgcta gcgtccgcgc gccagcgcgt aaggccccgg gtcaatcgcc
     1441 gtggctgcgc cgctgatgag ttgcgccagc aggcggccgg agccggcgcc ctgcgtccag
     1501 cccaggtggc cgtgcccgca gttgagccac aggccggcaa ccggcgtggc gccgatgtag
     1561 gggcggccat ctgcggccat cggccgcagt ccgcaccacg ctactgcgcg ttggcggtcg
     1621 gcgtcggcct caagcgacgg gccgagcgca cgcaggtagc gccacaggtt gtgcaggcgc
     1681 tcgggccgca gcgagaaatc gaacccgcag aattccgccg tgccggccag gcgcagaacc
     1741 gcgccaagcg gcgtggccga ggtgtgctgg ctgtcgtcga tgatcgccat cagcggcatg
     1801 cgcgccgcgc cgcgcatcgg taccgacagt gaatagcctt tcgccgggcg gatcgccagc
     1861 tttatgccga gcggcgcagc gagttgcggc gaccaggcgg cggccgcgag cacaatatgc
     1921 gcggcatcga tcaggcccgc ggacgtttcc acgccgcgca ccgcgccgcg cccggcgaca
     1981 atgcggcgca cctcgcagcc gaatgtgaat ttcacgccgc gccgctccag cgcgctgcgc
     2041 aggcccaggc accagcgatg cgcgtc
//
LOCUS       DASZZC010000455         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_615231,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000455 DASZZC010000000
VERSION     DASZZC010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615231"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="VHE37_09590"
     CDS             <1..1787
                     /locus_tag="VHE37_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVT35826.1"
                     /translation="LLQKGLIAVEDKGFYHHWGISIRGIFRAAFHNLMAGHAVEGAST
                     LTQQLVKNFFLTPDRSFKRKLTEVCMALLLELHYSKDEILEAYMNEIQLGQDGGRSVN
                     GFGLGAQFYFNKPVSELRSHEIALLVALAKGTSYYNPHRNPQRALERRNLVLGIFKEE
                     GLISEAEYDSAIARPLGLAGSKSQGADRYPAFVDLVKRQLRTQYKDEDLTHEGLRIFT
                     TLEPRAQEVLEQRITQGLPVVEKSRKLAEGTLQGAGVITSVEGGEVRALVAGRDVRYA
                     GYNRALDSRRSIGSLAKPFVYLTALERPSEYNLYSILPDDPIELKLPNRTTWSPNNYD
                     HKLHGPQPLYMALAQSYNLPTVALGLKLGPDAVLDTMHKAGYTGDAQALPSIFLGAVD
                     AAPIEVAQMYATLAASGYQSPLSAIREVQTKEGEPLTRFPIEVKATLPEAPVYLLTWA
                     MEHVLTLGTARSAYNVISPDIVLAGKTGTTDDLRDSWFAGFSGDRVAVVWVGRDDYKP
                     MGLTGAQGALPIWTQVMHDLRTKSLDVTPPAEVDEQLTDVDTGLRADERCTGAITVPY
                     LHGFVPEQAAPCAGGATTPLEWLKNIFK"
BASE COUNT          345 a          664 c          626 g          292 t
ORIGIN      
        1 aactgctgca gaagggcctg atcgcggtgg aggacaaggg cttctatcac cactggggca
       61 tcagcatccg cggcattttc cgcgccgcgt tccacaacct gatggccggc cacgcggtcg
      121 agggcgccag cacgctcacg cagcagctgg tgaagaactt cttcctcacg ccggaccgca
      181 gcttcaagcg caagctcacc gaagtctgca tggcgctgct gctggaactg cattactcga
      241 aggacgaaat cctcgaggcg tacatgaacg agatccagct cggccaggac ggcgggcgct
      301 cggtcaatgg cttcggcctg ggcgcgcagt tctacttcaa caagccggtg tcggaactgc
      361 gctcgcacga gattgcgctg ctggtggccc tggccaaggg caccagttac tacaacccgc
      421 accgcaaccc gcagcgcgcg ctggagcggc gcaacctggt gcttggcatc ttcaaggaag
      481 aaggcctgat cagcgaggcc gagtacgaca gcgccatcgc gcggccgctg ggcctggccg
      541 gcagcaagag ccagggcgcc gaccgctacc cggcgttcgt cgacctggtc aagcgccagc
      601 tgcgcacgca gtacaaggac gaggacctca cgcacgaagg cctgcgcatc ttcaccacgc
      661 tggagccgcg cgcgcaggaa gtgctggagc agcgcatcac ccagggcctg ccggtagtgg
      721 agaaatcgcg caagctggcc gagggcacgc tgcagggcgc aggcgtcatc accagcgtgg
      781 aaggcggcga ggtgcgcgcg ctggtggccg ggcgcgacgt gcgctacgcc ggctacaacc
      841 gtgcgctgga ttcgcgccgc tcgatcggct cgttggccaa gcccttcgtc tatctcaccg
      901 cgctggagcg gccgtcggaa tacaacctgt acagcatcct gccggatgat cccatcgagc
      961 tgaagctgcc caaccgcacc acctggtcgc ccaacaacta cgaccacaag ctgcacggcc
     1021 cgcagccgct ctacatggcg ctggcgcagt cgtacaacct gccgacggtg gcgctgggcc
     1081 tcaagctggg gccggacgcg gtgctcgaca ccatgcacaa ggccggctac accggcgacg
     1141 cgcaggcgct gccgtcgatc ttcctcggcg cggtcgatgc cgcgccgatc gaagtggcgc
     1201 agatgtacgc cacgcttgcc gccagcggct accagtcgcc gctgtcggcc atccgcgagg
     1261 tgcagaccaa ggaaggcgag ccgctgacgc gtttccccat cgaggtgaag gccaccttgc
     1321 ccgaggcgcc ggtctacctg ctcacctggg cgatggaaca cgtgctcacg ctgggcaccg
     1381 cgcgctccgc ctacaacgtg atctcgcccg acatcgtgct cgccggcaag accggcacca
     1441 ccgacgacct gcgcgacagc tggttcgccg gcttctccgg cgaccgcgtg gccgtggtct
     1501 gggttggccg cgacgactac aagccgatgg ggctcactgg cgcgcagggc gcactgccga
     1561 tctggaccca ggtgatgcac gacctcagga ccaagagcct cgacgtgacg ccgccggcgg
     1621 aagtcgacga gcagctcacc gatgttgaca ccggcctgcg cgccgacgag cgctgcaccg
     1681 gcgccatcac cgtgccgtac ctgcacggct tcgtgccgga gcaggcggcg ccatgcgcgg
     1741 gcggtgcgac cacgccgctg gaatggctga agaacatctt caagtagccg cggtcagtac
     1801 aatagcggtc cagttttctt cgcccgattc gagagtctca tgcggctgct agttcccatt
     1861 ttgcccgtgc tgtgcgtggt gcttgccggc tgccagacca cctatggcaa gcgcacgcaa
     1921 tggccgt
//
LOCUS       DASZZC010000456         5649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_615325,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000456 DASZZC010000000
VERSION     DASZZC010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615325"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..443
                     /locus_tag="VHE37_09595"
     CDS             171..443
                     /locus_tag="VHE37_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35827.1"
                     /translation="MIPKRSASKSRAPRRREPVNVELTVRFNFGQQPEDAVTVIDHRR
                     IPMVDSVFRSRDAILRGFVSLLVKAGLKQPAVVRELIPFTRKRASK"
     gene            440..736
                     /locus_tag="VHE37_09600"
     CDS             440..736
                     /locus_tag="VHE37_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35828.1"
                     /translation="MKYLRGFDLVLAAFGATMTITLGVVCIMYRVYIDQSPQMREEFP
                     ALLKVTLMFLLLAAAAGLAWQGLRRESAWRWPAQGALLAVIAGIIYGIRAVLLA"
     gene            845..2278
                     /locus_tag="VHE37_09605"
     CDS             845..2278
                     /locus_tag="VHE37_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT35829.1"
                     /translation="MIEGGYSELELPKPKQYEKLSWVPILAGLYWLTASQGGHWLVFA
                     AVPGSLLLMSGLALFLMPGNPRITAYMALAAALGIVLSLLAMFSGGFAAGFVAMLGSM
                     ASFLTAGRVGLMREPLYKGAVPPVFSSQLDLKAALDEFVLGYFVLAGRLPSGELVQPM
                     VDAVARLEGIFRERGWEEDPRGFHPAPPPPERTWFEHGRIFGMDYDVLRFDSGYAPED
                     ALPGAALWKSHTRNYQCAVRILRHEGPPRPWIFCIHGYRMGEAWMDFGLFPPQVLHEK
                     LGLNVIQPVLPLHGPRRIGPKSGDHFLDWDLLDLVYAEGQTLWDLRRTLAWLRAQEPD
                     ARVGVLGYSLGGYNASLLAAYEKQLDFVIAGIPVTDFSTALWRFIPTQHREFLESHGL
                     TSKRYRDLLRVVSPLQVKPLLDKERLLIYAATGDRIVPPWHPLQLGKHWDVPITWYQG
                     SHLSVRRERETRQVLKEAMLRAGWLVG"
     gene            2356..3477
                     /locus_tag="VHE37_09610"
     CDS             2356..3477
                     /locus_tag="VHE37_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HVT35830.1"
                     /translation="MKRVAITGSGLYTPPESISNDELVAAYNAYVERYNAQHAAEIAA
                     GSAQPLPPSSSEFIVKASGIQSRHVVNKSGVLDIDVMRPQIRNRGNDEPSIMCEMATA
                     AASQALEHAGRKAADVDAVLVACSNMQRPYPAIAVEVQNALGIGGYGYDINVACASAA
                     FGVQAAVDAVRNGSARAVLVVSPEICSGHLNFRNRDCHFIFGDAATALLVEPLEGSNG
                     ADRFEVLGTRLKTQFSNNIRNNFGFLNSAEVPPRPWNDVLFAQEGRKVFKEVVPMVSE
                     LITAHLQELGLAPDHVKRFWLHQANLGMNQLISRKVLGREPSVEEAPVILDEYANTSS
                     AGSVIAFHKFHRDFQPGDFGVLCAFGAGYSAGSVVLRRA"
     gene            3573..3833
                     /gene="rpsP"
                     /locus_tag="VHE37_09615"
     CDS             3573..3833
                     /gene="rpsP"
                     /locus_tag="VHE37_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HVT35831.1"
                     /translation="MVTIRLARAGAKKRPFYHVVATDKRKARDGRFIERLGFYNPGAT
                     GAEAKLLVDAARFEHWTKVGAQVSERVTFLLKNVKQEAPKAA"
     gene            3847..4353
                     /gene="rimM"
                     /locus_tag="VHE37_09620"
     CDS             3847..4353
                     /gene="rimM"
                     /locus_tag="VHE37_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HVT35832.1"
                     /translation="MTLGRIAGVYGVQGWLRIHSETRPQENLLKYRSWLIKSPQAEFQ
                     AKLLQGRMHSGAVIAQISGPDGAAITDRDAAAALNGAQIQVERSTLPKLKKGQYYWVD
                     LIGLQVQNEQGAALGTVTDMTSNNVQDVLVVKDGGTERLIPFVNGAVVKSVDLKARRI
                     VCDWQPDW"
     gene            4358..5116
                     /gene="trmD"
                     /locus_tag="VHE37_09625"
     CDS             4358..5116
                     /gene="trmD"
                     /locus_tag="VHE37_09625"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HVT35833.1"
                     /translation="MKIEVVTLFPELVEQVGRHGMPRIALEAGALQLTTRNPRDYSGN
                     RWRRVDDRPYGGGPGMVMEAEPLLQAIEAAKAVTGPARVVALSPQGERFNQAWAGRLS
                     REAGLVLVCGRYEAIDERLTADAAGAIDLELSLGDFIMSGGELAAMAVIDAIARLLPG
                     VLGGVESAGADSFADGALDHPHYTRPEVWRNLAVPEVLMSGDHAAIRRWRRQQALGAT
                     WLKRPDLLDGLQLDEESQALLREFISQRQELKTR"
     gene            5120..5476
                     /gene="rplS"
                     /locus_tag="VHE37_09630"
     CDS             5120..5476
                     /gene="rplS"
                     /locus_tag="VHE37_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HVT35834.1"
                     /translation="MRTNKIIAAVEAEQMGSKKVPDFRPGDTVEVKVKVKEGDRERLQ
                     AFEGVVIAKRNRGLNSAFTLRKHSHGEGVERVFQTYSHQVAEISVKRRGQVVRSKLYY
                     LRDRAGKSARIAEKLD"
BASE COUNT          957 a         1758 c         1974 g          960 t
ORIGIN      
        1 aactgctgca cgcgcagccg cgcatcatgc gtctgactat aatccgggct cgtcaccgcg
       61 aagccgcggc gccgcgccac cccggccagg tgcgtgatct tggtgcccca ggggccgctt
      121 tccttgccgt ggttgaagca caccaggttc tgttcgctca tagccatccg atgataccca
      181 agcgatctgc ctccaaatcc cgcgcaccgc gccggcgcga gccggtcaac gtcgagttga
      241 ccgtgcgctt caacttcggc cagcagcccg aggacgcggt cacggtgatc gatcaccgcc
      301 gcatcccgat ggtcgacagc gtgttccgca gccgtgacgc catcctgcgc ggcttcgtca
      361 gcctgctggt caaggccggg ctgaagcagc cggcggtggt gcgcgagctg atcccgttca
      421 cccgcaagcg tgcatcgaaa tgaaatacct gcgcggattc gacctggtgc tggccgcgtt
      481 cggcgccacc atgaccatca cgctgggcgt ggtgtgcatc atgtaccgcg tgtacatcga
      541 ccagtcgccg cagatgcgcg aggagttccc ggcgctgctc aaggtcacgc tgatgttcct
      601 gctgctggcg gccgcggcgg gactggcctg gcagggactg cgccgcgaaa gcgcctggcg
      661 ctggcccgcg cagggggcgc tgctggcggt gatagccggc atcatctacg gcatccgcgc
      721 tgtgctgctg gcctgaaacg cgcgaaaaac agcccgaccg caccaccatt ttcgatgtta
      781 tttattactg catttaagta aataaaatca attttttata acaacacgaa tccggggaat
      841 tttcatgatt gaaggcggct acagcgaact ggaactgccc aagcccaagc agtacgagaa
      901 gctgagctgg gtgccgatcc tggccgggct ttactggctc accgcgtcgc agggcggcca
      961 ctggctggta ttcgcggcgg tgcctggcag cctgctgctg atgagcggcc tggcgctgtt
     1021 cctcatgccg ggcaatccgc gcatcacggc gtacatggcg ctcgcggccg cgctgggaat
     1081 cgtgctgtcg ctgctggcca tgttcagcgg cggatttgcg gcgggcttcg tcgccatgct
     1141 ggggtccatg gccagcttcc tcaccgccgg acgtgtcggc ctgatgcgcg agccgctgta
     1201 caagggcgcg gtgccgccgg tgttttcctc gcagctcgac ctgaaggcgg cgctcgacga
     1261 attcgtgctc ggctacttcg tgctcgccgg ccggttgccg agcggcgaac tggtgcagcc
     1321 gatggtcgac gcggtggcgc ggctcgaggg catcttccgg gaacgcggct gggaggagga
     1381 cccgcgcggc ttccatcccg cgccgccgcc gcccgagcgc acctggttcg agcatggccg
     1441 cattttcggc atggattacg acgtgctgcg ctttgacagc ggctacgcgc cggaagacgc
     1501 gctgcccggc gccgcgctgt ggaagagcca cacgcgcaac taccagtgcg cggtgcgcat
     1561 cctgcgccac gagggtccgc cgcggccgtg gatcttctgc atccatggct accgcatggg
     1621 cgaggcgtgg atggacttcg gcctgttccc gccgcaggtg ctgcacgaga agctgggcct
     1681 caacgtgatc cagccggtgc tgccgctgca cggcccgcgg cgcattgggc cgaagtcggg
     1741 cgatcatttc ctcgactggg atctgctgga cctggtctat gccgaaggcc agacactgtg
     1801 ggacctgcgc cgcacgctgg cctggctgcg cgcgcaggag ccggatgcgc gcgtgggcgt
     1861 gctcggctac agcctgggcg gctacaacgc ctcgctgctg gcggcgtatg aaaagcagct
     1921 ggatttcgtc attgccggca tcccggtcac ggatttttcc accgcgctgt ggcgcttcat
     1981 cccgacgcag caccgcgagt tcctggaatc gcacgggctg accagcaagc gctaccgcga
     2041 tctcctgcgc gtggtgtcgc cgctgcaggt caagccgctg ctcgacaagg agcgcctgct
     2101 gatctatgcc gccacgggtg accgcatcgt gccgccgtgg cacccgctgc agctgggcaa
     2161 gcactgggac gtgccgatca cctggtacca gggttcgcac ctgtcggtgc gacgcgagcg
     2221 cgagacacgc caggtgctga aggaagcgat gctgcgcgcc ggctggctgg tgggctgagg
     2281 agttctgccc gcgcggagtt cgccggcgag cgggtaaact tcgcgtcctt attttatgca
     2341 gcggggcacg ggcacatgaa acgagtggcc atcaccggca gcggcctgta cacgcccccg
     2401 gaatccatct ccaacgatga gctcgtcgcc gcctacaacg cgtacgtcga gcgctacaac
     2461 gcccagcatg ccgcggagat tgccgcgggc agcgcgcagc cgctgccgcc gtcgtcgagc
     2521 gagttcatcg tcaaggcttc cggtatccag tcgcgccacg tggtcaacaa gtccggcgtg
     2581 ctcgacatcg acgtgatgcg gccgcagatc cgcaaccgcg gcaacgacga gccttcgatc
     2641 atgtgcgaga tggcgaccgc cgcggccagc caggcgctgg agcatgccgg gcgcaaggcc
     2701 gccgacgtcg atgctgtgct ggtggcctgt tccaacatgc agcggcctta cccggccatc
     2761 gcggtggaag tgcagaacgc gctgggtatc ggcggctacg gctacgacat caacgtggcc
     2821 tgcgcttccg cggcgttcgg tgtgcaggcg gcggtcgacg cggtgcgcaa cggcagtgcg
     2881 cgcgcggtgc tggtggtgag cccggagatc tgctcgggcc atctcaattt ccgcaaccgc
     2941 gactgccatt tcatcttcgg cgacgccgcc accgcgctgc tggtcgagcc gctggaagga
     3001 tcgaacggcg ctgaccgctt cgaagtgctg ggtacgcggc tgaagacgca gttctccaac
     3061 aacatccgca acaacttcgg cttcctcaat tccgccgagg tgccgccgcg gccgtggaac
     3121 gacgtgctgt tcgcccagga aggccgcaag gtgttcaagg aagtggtgcc gatggtgtcg
     3181 gagctgatca ccgcgcacct gcaggaactg ggcctcgcgc ccgaccacgt caagcgcttc
     3241 tggctgcacc aggccaacct gggcatgaac cagctgatca gcaggaaggt gctgggccgc
     3301 gagcccagcg tggaagaggc gccggtgatc ctcgacgaat acgccaacac cagttcggcc
     3361 ggctcggtca ttgccttcca caagttccac cgtgatttcc agccgggtga cttcggcgtg
     3421 ctgtgcgcgt tcggcgccgg ctattcggcg ggcagcgtgg tgctgcggcg ggcctgattg
     3481 gcagtggtat ttcgccgata aatcgttaaa atacgcgccc ttttggcacg cccggacctg
     3541 ttgcgggcga gccggtcact ggaatttcga gcatggttac cattcgtctt gcccgcgctg
     3601 gcgccaagaa gcgcccgttc tatcacgtgg ttgcgaccga caagcgcaag gcgcgcgacg
     3661 gccgcttcat cgagcgcctg ggcttctaca accccggcgc taccggcgcg gaagcgaagc
     3721 tgctggtcga cgcggcgcgc ttcgagcact ggaccaaggt gggcgcgcag gtgtccgagc
     3781 gcgtgacctt cctgctgaag aacgtgaagc aagaggcgcc caaggcggcc tgatgtgagc
     3841 acgcgcgtga cgctgggcag gatcgccggc gtttacggag tgcagggctg gctgcggatc
     3901 cattcggaga cgcggccgca ggaaaatctc ctgaagtacc gcagctggct gatcaagtcg
     3961 ccgcaggcgg aattccaggc gaagctgctg caggggcgga tgcacagcgg cgcggtcatc
     4021 gcgcagatca gcgggccgga cggcgcggcg atcaccgacc gggatgcggc ggcagcgctc
     4081 aacggcgcgc agatccaggt cgagcgcagc acgctgccga agctgaagaa gggacagtat
     4141 tactgggtgg acctgatcgg tctgcaggtg cagaacgagc agggcgcggc actgggcacg
     4201 gtgaccgaca tgaccagcaa caacgtgcag gacgtgctgg tggtgaagga cggcgggacg
     4261 gaacggctga tcccgttcgt caacggtgcg gtggtgaagt cggtggacct gaaggcgcgg
     4321 cgcatcgtgt gcgactggca gccggactgg taggccgatg aagattgaag tggtcacgct
     4381 gttccccgaa ctggtggaac aggtggggcg ccacggcatg ccgcgcattg cgctggaagc
     4441 cggggcgctg cagctgacga cgcgcaatcc gcgcgactac agcggcaata ggtggcgccg
     4501 ggtcgatgac cgcccttacg gtggcgggcc cgggatggtg atggaagcgg aaccgttgct
     4561 gcaggcgatc gaggctgcca aagcggtgac cggtccggcc agggtggtgg cgctgtcgcc
     4621 gcagggcgag cgcttcaacc aggcctgggc cgggcggctc agccgcgaag cggggctggt
     4681 gctggtatgc gggcgctacg aagccatcga cgaacggctg acggcggacg cagcgggtgc
     4741 gattgatctg gagttgtcgc tgggcgattt catcatgtcc ggcggcgaac tggcggcgat
     4801 ggcggtgatc gacgcgattg cgcggttgct gccgggcgtg ctgggcgggg tggagtcggc
     4861 gggcgcggat tcgtttgccg atggcgcgct ggaccacccg cactacacga ggccggaggt
     4921 gtggaggaat ttggcggtgc ccgaggtgct gatgtcgggc gaccatgcgg cgatccgccg
     4981 ctggcggcgg cagcaggcgc ttggagcaac gtggttgaag cggcccgacc tgctggacgg
     5041 gctgcagctg gatgaagagt cacaggcgct gttgcgggag ttcatttcgc agcggcagga
     5101 attgaaaacg aggtgagtca tgcggaccaa caagatcatt gcggcggttg aagccgagca
     5161 gatgggcagc aagaaggtgc cggacttccg gcccggcgac accgtcgagg tgaaggtgaa
     5221 ggtcaaggaa ggcgaccgcg agcgcctgca ggccttcgag ggcgtggtca tcgccaagcg
     5281 caaccgcggc ctgaacagcg ccttcacgct gcgcaagcac tcgcacggcg aaggcgtcga
     5341 gcgcgtgttc cagacctaca gccaccaggt cgccgaaatt tcggtgaagc gccgtggcca
     5401 ggtggtgcgc agcaagctct actacctgcg cgaccgcgcc ggcaagagcg cgcgcatcgc
     5461 cgaaaagctc gactgagttc ccgccggaaa caaaccaccc gccgcgcgca agcccggcgg
     5521 gttttttgtt tttggaagat agccgggcga ccgcctggcc ttggcggtac gcgccacgga
     5581 tggggcgttc ttgcccagga tggcgtattg acattaaagt gatatcattt taatttcatc
     5641 cgcatcaac
//
LOCUS       DASZZC010000457        15818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_943324,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000457 DASZZC010000000
VERSION     DASZZC010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 15818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15818
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943324"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /gene="mnmA"
                     /locus_tag="VHE37_09635"
     CDS             complement(<1..728)
                     /gene="mnmA"
                     /locus_tag="VHE37_09635"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVT35835.1"
                     /translation="MKQHVVVGMSGGVDSSVAALLLQQQGYRVSGLFMVNWTEDEEAY
                     CSSAQDYQDALAVCAELKIPLHRADFSREYRERVFAHFLADYAAGKTPNPDVLCNREV
                     KFQPFREHALRLGADFVATGHYARIEHTDEGPRLLRSVTEDKDQTYFLAAVDRAQLER
                     VLFPVGGLTKPQVRELAQGAGLPNHRKKDSTGICFIGEREFSSFLQQYLKPNPGNMLD
                     DGGAKVGTHQGLMHYTLGQRRGLR"
     gene            complement(790..1233)
                     /locus_tag="VHE37_09640"
     CDS             complement(790..1233)
                     /locus_tag="VHE37_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVT35836.1"
                     /translation="MSWSPHVVVASIVERDGKFLLVEERVNERLVLNQPAGHWEQGET
                     LIEAARREALEESAWDVEPTALLGIYHYQPENLAYSFLRVAFVAEARKHHPQRKLDDG
                     IERAVWLTADEIAAQHARHRSPMLMRCIDDYLAGRRYPLSILTHL"
     gene            complement(1238..1999)
                     /locus_tag="VHE37_09645"
     CDS             complement(1238..1999)
                     /locus_tag="VHE37_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HVT35837.1"
                     /translation="MTRRWLIALAIAGFLYGMLMRAPAATLAWYGLVKSGGGTELYGL
                     QGTLGEGTLTGLQFGGRRVTMDLRWTLKPLWLLLGQARFHVTGSGSDTATSGDVALAP
                     LHTVRLRSFQASGGLKPVLAAAGQAFLPVDGQVSVDADQVTFNKGVLQNLAGTVHIQQ
                     LMWTLARDPVALGDFQAQVSSDKADSVAKIESTGGPMELSGDGRLGADQTWQVDLKFR
                     LKSDATPMLRNLVAGAGAPDAQGWYHLKQSGKLKS"
     gene            complement(1996..2502)
                     /locus_tag="VHE37_09650"
     CDS             complement(1996..2502)
                     /locus_tag="VHE37_09650"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HVT35838.1"
                     /translation="MNAYIQQLRDWLATLAPRERVLVLVAAVCAGIALLDLALWEPLV
                     HAHEKREKSLAESRALAQRLESLAPDAMRARGGGGGANAALRSMSLLAAVDQSAKSGT
                     LSKPPTRLQPEGDDEVKVWLEATAFDSVLRWINELDTRYGITAQTIDIDKESTPGQVN
                     VRLSLVRS"
     gene            complement(2499..3704)
                     /gene="gspL"
                     /locus_tag="VHE37_09655"
     CDS             complement(2499..3704)
                     /gene="gspL"
                     /locus_tag="VHE37_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HVT35839.1"
                     /translation="MRETLYIRLRSTAPEAVTAYCIAPANALRSFHAQEAPLAEVLAL
                     AGTRRIVLLVPGADVRLAQVKVPARAPAKVLQAAPYLLEEQLAEDVDTLHFALGPRQA
                     DGQFPVAVAARERVEAWLAPLRERHLTPEAIYPETLCLPEPDTAHWSALAEEDHVTVR
                     TQRYAGFACAPDDLPLLLELSAAPAQTTLRILVARGVERDLSRLSRPVELLPAYNTAL
                     EALLQNLDASSAINLLQGAYSPRQNIQRLWLPWRAAAALALAWLLAAGTTHAVAAMKL
                     GKELDKQNDLNLQRFKQLFPAEQRIVDLDAQVEQQSRLLKGGAHKGGFLPLLQTLTSA
                     LATTQGLNVQGLQFREGALYASLGGSDLQQLETLRSWFAQHHDAALEVQSANSGAEGV
                     QIRLKLTPA"
     gene            complement(3705..4640)
                     /gene="gspK"
                     /locus_tag="VHE37_09660"
     CDS             complement(3705..4640)
                     /gene="gspK"
                     /locus_tag="VHE37_09660"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HVT35840.1"
                     /translation="MKAQRGVALITAILVVALAAIAAAAVLSSAMMAVHRAANMQDSE
                     KAWWYADGVESWLKSVLQRDAQDNKTDSLKDDWAKPVDYLPVDEGYVRGAVIDLQGRF
                     NLNNLGVADPNLYAKYVDQFERLVQNIEGADAMQARPLAAAIRDWIDPDQTPTGMDGA
                     EDSEYLSMDPPYRAANRYLESVSELRAIKGMDAKLYGLLRNYVAALPQIGTPVNINTA
                     PEPVLRSLVKQTNPDLEKFLATREEQPADSTAALQTMIGPETPMGLAGISVKSNYFMS
                     RVETFIGSGRLALYSFYYRPDQGAPVVLGRSTDTE"
     gene            complement(4637..5347)
                     /gene="gspJ"
                     /locus_tag="VHE37_09665"
     CDS             complement(4637..5347)
                     /gene="gspJ"
                     /locus_tag="VHE37_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HVT35841.1"
                     /translation="MKRQPGFTLLELVVAIGIFALVALMAYGGLNSVLKGRRQVEESL
                     DRTGEFQRAYRWLRDDFQQVRTRPARDAYGDAQPALRGDHDTRVEFTRGGWRNPAELP
                     RPGLERVDYILQDKKLMRESFRVLDQGQDSKAVQSELLDQVEDMSLRYLSASREWSDT
                     WPPPDNTGGAQQKTSPPPPMAVEMSLRTKDWGDLRFLFRIGLDTPPQGYTPGAASGAP
                     TTTPTTTVTSTTTPKGQQ"
     gene            complement(5344..5724)
                     /gene="gspI"
                     /locus_tag="VHE37_09670"
     CDS             complement(5344..5724)
                     /gene="gspI"
                     /locus_tag="VHE37_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HVT35842.1"
                     /translation="MKSHRRLRGFTLIEILVAVTIIAVALGAIVRGLAMYASNAGYLR
                     QKTLATWVAHDRLTEIELEPPWPAEGKSDGDADMGGFKWRWFVEVKKTPDEHLRRIDI
                     RVQLPGKELDIASMSAFISDTGRQ"
     gene            complement(5721..6263)
                     /gene="gspH"
                     /locus_tag="VHE37_09675"
     CDS             complement(5721..6263)
                     /gene="gspH"
                     /locus_tag="VHE37_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HVT35843.1"
                     /translation="MRPTRMLRQSGFTLIEMMVVTIVIGIIATFFSFSMGHRVMDDRM
                     DNEAARMERILQLASEEAETKGIDIGVRYTPERMELLARDDDGNWKEYAEGGPLRTRA
                     VSDPFYTELFVEGRAVAPAQDSQDPDKQKKIEPQFFLLSSGEVTPFALNVRARGYKPY
                     YHIEADALGHFKLERHEEQG"
     gene            complement(6281..6748)
                     /gene="gspG"
                     /locus_tag="VHE37_09680"
     CDS             complement(6281..6748)
                     /gene="gspG"
                     /locus_tag="VHE37_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HVT35844.1"
                     /translation="MNKTSFRPVARARHAAGFTLIEIMVVVVILGILAAVVVPKIMDA
                     PVKARIAKAKQDISALESALEYYKLDNFSYPSTQQGLEALVNKPGGDPQPHNYKAGGY
                     IKSLPKDPWGNPYQYLSPGVKGEIDIFSYGSDGKPGGDGEATDIGSWDLTSTN"
     gene            6847..7398
                     /gene="rrtA"
                     /locus_tag="VHE37_09685"
     CDS             6847..7398
                     /gene="rrtA"
                     /locus_tag="VHE37_09685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HVT35845.1"
                     /translation="MLLLLGTELLGDWGREHLRYDRAAIAAGQWWRFITGNFVHLGWY
                     HWALNEAGIVVLVLLCPEQLSPLVWMRRVLLLCVGMSAGLYWLVPGLPTYVGMSGVIH
                     GLFVLGLMPQVLKKDLIALGCLLYLLGKLGYELYAGAPVSDEHAIGGSVVTQAHFYGS
                     ISALFYGLVFRTYTRHETWRLKS"
     gene            7395..8339
                     /gene="fabD"
                     /locus_tag="VHE37_09690"
     CDS             7395..8339
                     /gene="fabD"
                     /locus_tag="VHE37_09690"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HVT35846.1"
                     /translation="MTAPSYAILFPGQGSQAVGMLGAHDAPVVARTFAEAGAALGWDL
                     AQLVRGGPEEELNQTARTQPALLAAGVALWRLWDESGLPRPAALAGHSLGEYTALVAA
                     GALDFADALRLVELRGQLMQAAVPAGTGGMAAVIGLDDAKVADLCKAYPGSEVLEPVN
                     YNAPGQVVVAGQAAALDWLAANGKAHGARMVMRLPVSVPSHCSLMRDAAQKLADKLAA
                     TSIHAPAIPVYHNLDAAPHSEPASIRRALTEQLYRPVLWTSTIQKLVASGIGTFVECG
                     PGKVLTGLTKRIADKSARYVSLEDPAGLQQALAWLKTA"
     gene            8446..9171
                     /gene="fabG"
                     /locus_tag="VHE37_09695"
     CDS             8446..9171
                     /gene="fabG"
                     /locus_tag="VHE37_09695"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HVT35847.1"
                     /translation="MNLQGEIALVTGASRGIGRAIAERLARDGAQVTGTATTDEGAAA
                     ITQYLAPMGGHGIRLDVRDAAAIEAALAQAGAVSILVNNAGVTRDTLLLRMKDEDWEQ
                     VLQTDLTSVFRLSRAVLRGMMKARHGRIVNIGSVVGAMGNPGQANYCAAKAGLVGFSK
                     SLAQEIGSRGITVNVVAPGFIDTDMTKGLGEDTRKALIDRVPVQRLGAPDDIAAAVAF
                     LASRDAGYITGETLHVNGGMYMN"
     gene            9303..9548
                     /gene="acpP"
                     /locus_tag="VHE37_09700"
     CDS             9303..9548
                     /gene="acpP"
                     /locus_tag="VHE37_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVT35848.1"
                     /translation="MSSLEEKVKKIIAEQLSVSEDQITPEASFVEDLGADSLDTVELV
                     MALEEEFEIDIPDEEAEKIVTFQDVINYIKSHATQQG"
     gene            9592..10833
                     /gene="fabF"
                     /locus_tag="VHE37_09705"
     CDS             9592..10833
                     /gene="fabF"
                     /locus_tag="VHE37_09705"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HVT35849.1"
                     /translation="MSRRRVVVTGMGIVSPVGSTVDSAWANILAGRSGITPITRYDIS
                     SFPTRFAGQVADFRAEEWMNPKDVRRVDEFIVYGVAAAKQAMKDSGLEVTDANRERIG
                     VCVGSGIGGIGTIEEQCMLLNNQGVKRVSPFFVPSSIINMISGHISIDLGITGPNLAT
                     VSACTTGAHGIGIGARLIAYGDADVFIGGGAEAGSAPAGMAGFCQARALSTRNDDPQR
                     ASRPWDKDRDGFVLGDGAGVLVLEEYEHAKKRGARIYAELIGFGMSGDAHHITAPPDD
                     GAGARRAMVAALKDAGINAGQVDYINAHGTSTPLGDVAETIAIKAAFGAHAHKVAVSS
                     TKSMTGHLLGAAGGVEAIFSVLALRDQVIPPTINLETPGEGCDLDYVPGTARQAKLSI
                     AISNSFGFGGTNGTLAFQTLN"
     gene            10847..12151
                     /locus_tag="VHE37_09710"
     CDS             10847..12151
                     /locus_tag="VHE37_09710"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HVT35850.1"
                     /translation="MSFRAEVTGQIDLFNLHRQQPEVFPFLLQSLGAGSGRYDLLFAF
                     PGDTIEGPEFFAGLNQQAAAKRCAPQDELPFCGGWFLLLGYEAARWIEPRLRLPASPH
                     SLPDALAVRCTAVAVYDHQRRSTTAYAESSQAELDEVVAALRSNLGSPAYAPGNGLQL
                     HVQADVDGEFEAGVRSILEYLKAGDVFQVNLSRAWRAWMDQRSSYLDLYARLRHANPA
                     PFAGLARWGDAVLLSSSPERLVEIRDGWVQTRPIAGTRPRGNGTGEDEQLRAALIASV
                     KERAEHIMLIDLERNDLGRVCQPGSVEVSELMTIESYAHVHHIVSNVRGRLRAEAGPG
                     DVLRAVFPGGTITGCPKVRAMEIIAELECVGRGPYTGAMGYLSRCGRMDTNILIRSLL
                     CESDTLTFRTGAGIVADSDPQAELAETRAKARGLLLALGAQP"
     gene            12148..12966
                     /gene="pabC"
                     /locus_tag="VHE37_09715"
     CDS             12148..12966
                     /gene="pabC"
                     /locus_tag="VHE37_09715"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HVT35851.1"
                     /translation="MSALYNGKPAESVPLHSRGLNYGDGVFRTLLVTDGRIHDWALQY
                     ATLARDAARLSLQLPEEGLLRSECDFLAADRRLAAMKVMLVRAGERRGYAPETSTCER
                     LVSVHDLPRYPSRHWSRGVAVIGSTVQLAQQPLLAGIKHLNRLEQVLASRDWPQDISE
                     ALMCDHAGRVAGGTRSNLFWVRQGRLYTPDLSRSGVDGMMKSKVTALARAHSMELRTV
                     DADWAELEQADEIFLTNSLVGIWPVRALEGRMLDAPGVVTARLSALLHHPLPIA"
     gene            12963..13988
                     /gene="mltG"
                     /locus_tag="VHE37_09720"
     CDS             12963..13988
                     /gene="mltG"
                     /locus_tag="VHE37_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVT35852.1"
                     /translation="MRRFLAGILLVPLLLAAAALLAWRDAQGTLAAPLPITEKFIYEI
                     PVGAPLQQVIADLRQRGVVLDRRLGLYLRIYAEVKKLNLRAGEYELTPGISAIELLTT
                     FASGKTLLHELRIVEGWTFAQAMKVIAADPDLAHSLEGKSPEQVMAQLGYTDTFAEGR
                     LFPDTYRFPKGLSDITFLRRAAATMDAMLQKEWDGRDADLPYASPADALIMASIVEKE
                     TGQASERPEVAGVFVNRLRSGMKLQTDPTIIYGLGPAFDGNLRKIDLETDGPYNTYTR
                     AGLPPTPICLPGRASLHAVLHPAQTKALYFVSRGDGTHVFSETYDAHSAAVQKFQIAP
                     HAAHGKK"
     gene            13975..14604
                     /gene="tmk"
                     /locus_tag="VHE37_09725"
     CDS             13975..14604
                     /gene="tmk"
                     /locus_tag="VHE37_09725"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HVT35853.1"
                     /translation="MARNKRGRFITLEGGEGAGKSTNARFIRDWLTARGREVVLTREP
                     GGSPMAEAIREVVLRDWKESMAPDTELLLMFAARASHLHSVILPALAAGKDVVCDRFI
                     DSTYAYQGAGKQMPVARIKLVDRMVLGTFAPDLTLLFDLPPEEGLKRAHARGEKNRFE
                     AESLAYMRRVRACFLQRARSAPGRYAVIRANRPLPAVQKTVARVLEQKL"
     gene            14601..15575
                     /gene="holB"
                     /locus_tag="VHE37_09730"
     CDS             14601..15575
                     /gene="holB"
                     /locus_tag="VHE37_09730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HVT35854.1"
                     /translation="MSVIPKPLPWQQELWVEVTALALQDRLGHALLLSGPAGVGKRAF
                     AKALTAFLLCEQRSGYACGRCRSCEQLAAGAHPNSIVLALEVDDKTGKQKRDISVEQV
                     REFSERLQLTSHYGQAKVATIDPADALNAAGVNALLKTIEEPSGHTHVLLISERAMAL
                     PATLRSRCQRLQFAPPAQQEAMAWLHEHHPEVGEQTLALAHGAPLRAAELAQSGLVER
                     YASWEKGMSDLAAGRGDPLALAAAIAKEKDDVPAYFSWLQLWLAGLLRGAVGAAPKKL
                     ALPAPALQQMMREAIDAQRRLGGNAVPQLLLESVIVLWWRLARPVKAA"
BASE COUNT         2592 a         5085 c         5328 g         2813 t
ORIGIN      
        1 atgcgcaggc cgcggcgctg gcccagcgtg tagtgcatca ggccctggtg ggtgccgacc
       61 ttcgcgccgc cgtcgtcgag catgttgccg ggattcggct tgaggtactg ctgcaggaag
      121 ctgctgaact cgcgctcgcc gatgaagcag atgccggttg aatccttctt gcgatggttg
      181 ggcaggcccg ccccttgcgc cagttcgcgc acctgcggct tggtcaggcc gccgaccggg
      241 aacagcacgc gctccagctg cgcgcggtcg accgcggcga ggaagtaggt ctggtccttg
      301 tcctcggtca ccgagcgcaa caggcgaggt ccttcgtcgg tgtgttcgat gcgcgcgtag
      361 tggccggtgg cgacgaagtc cgcgcccagg cgcagcgcat gctcgcggaa cggctggaac
      421 tttacctcgc ggttgcacag cacgtcgggg ttgggcgtct tgccggccgc gtagtcggcg
      481 aggaaatgcg cgaacacgcg ctctcggtat tcgcgcgaaa aatcggcgcg gtgcagtgga
      541 atcttgagct cggcgcagac agcgagcgcg tcctggtaat cctgcgcact gctgcagtaa
      601 gcctcttcgt cctcggtcca gttgaccatg aacaggccgg acacgcggta tccctgttgc
      661 tgcagcagca gggcggcgac ggaggaatca acacctcccg acatgccgac cacgacgtgt
      721 tgcttcatgc ttttattgta ggagcgcgcc tggcgcgcga tgcagtttga tcgcgcgcaa
      781 ggcgcgctcc tacaggtgag tgaggatgga caatgggtag cggcgccccg ccaggtagtc
      841 atcgatgcag cgcatcagca tcgggctgcg gtggcgcgcg tgctgtgcgg cgatttcgtc
      901 ggcggtcagc cagaccgcgc gctcgatgcc gtcgtcgagc ttgcgctgcg gatgatgctt
      961 gcgggcctcg gcgacgaagg ccacgcgcag gaaactgtaa gcgagattct cgggctggta
     1021 gtgatagatg ccgagcaggg cggtgggctc gacgtcccaa gcgctttcct ccagcgcttc
     1081 gcggcgtgcg gcttcgatca acgtctcgcc ctgttcccaa tggccggcgg gctggttcag
     1141 caccaggcgc tcgttgacgc gctcctcgac cagcaggaat ttcccgtcgc gctcgacaat
     1201 gcttgcgacc accacgtgcg gcgaccagct catagggtca ggacttcagc ttgccgcttt
     1261 gcttgaggtg gtaccagccc tgcgcatcgg gcgcgccggc gccggcgacc aggttgcgca
     1321 gcatcggcgt ggcgtctgac ttgaggcgga acttcaggtc cacctgccag gtctggtcgg
     1381 cgcccaggcg cccgtcgccg ctcaattcca tcgggccgcc ggtcgattcg atcttcgcca
     1441 cgctgtcggc cttgtcgctg ctgacctgcg cctggaaatc gccgagcgcg accggatcgc
     1501 gcgccagtgt ccacatcagc tgctggatgt gcacggtgcc tgcgagattc tgcaggacgc
     1561 ccttgttgaa ggtgacttgg tcggcgtcga cgctgacctg tccgtcgacc ggcaggaacg
     1621 cctgtccggc tgccgccagc acgggcttca ggccgccgct ggcctggaat gagcgcaggc
     1681 gcacggtgtg cagcggcgcg agcgccacgt cgccgctggt ggcggtatcg ctgccactgc
     1741 cggtcacgtg aaagcgcgcc tggcccagca acagccacag aggcttgagc gtccagcgca
     1801 ggtccatggt cacacgtcgg ccaccgaact gcaggccggt cagcgtgcct tcgccgagcg
     1861 tgccctgcag cccgtacagc tcggtgccgc cgccggactt gaccaggcca taccacgcca
     1921 gcgtcgcggc cggcgcgcgc atcagcatgc cgtaaaggaa tcccgcgatg gccagtgcga
     1981 tcagccagcg acgcgtcatg agcgcaccag cgaaaggcgc acgttgacct ggcccggcgt
     2041 cgattccttg tcgatgtcga tggtctgcgc ggtgatgcca tagcgcgtgt cgagttcgtt
     2101 gatccagcgc agcacgctgt cgaaggcggt agcttcgagc cacactttca cctcgtcgtc
     2161 gccttccggc tgcaggcgcg tcggcggctt ggacagggtg ccggacttgg ccgactggtc
     2221 caccgccgcc agcagcgaca tgctgcgcag cgccgcgttg gccccgccgc cgccaccgcg
     2281 cgcacgcatc gcgtcgggag cgagcgattc caggcgctgc gccagcgcgc gcgattccgc
     2341 caggctcttc tcgcgcttct cgtgcgcgtg caccagcggc tcccacaggg cgagatcgag
     2401 cagcgcgatg ccggcgcaca cggccgcgac cagcaccagc acgcgttcgc gcggagcgag
     2461 cgtggccagc caatcgcgca attgctggat gtaggcgttc atgccggcgt caatttcaaa
     2521 cggatctgca cgccttcggc gccggaattg gcggactgca cttcgagcgc cgcgtcgtgg
     2581 tgctgggcga accagctgcg cagggtttcc agctgctgca gatcgctgcc gccgaggctt
     2641 gcgtacagcg cgccttcgcg gaactgcagc ccctgcacat tcaggccctg cgtagtggcg
     2701 agcgcgctgg tcagcgtctg cagcagtggc aggaagccgc ccttgtgtgc gccgcccttg
     2761 agcaggcggg attgttgctc gacctgcgcg tcgagatcga cgatgcgctg ctcagccggg
     2821 aacagctgct tgaagcgctg caggttcagg tcattctgct tgtccagttc cttgcccagc
     2881 ttcatcgcgg cgacggcgtg cgtggtgccg gccgcgagca gccaggccag cgcaagcgcg
     2941 gccgcagcgc gccacggcag ccacaggcgc tggatgttct gtcgcggcga atacgcgccc
     3001 tgcagcaggt tgatggcgct gcttgcgtcc aggttctgca acagcgcttc caaagcggtg
     3061 ttgtacgcag gcagcagttc caccggccgc gacaggcgcg acaggtcgcg ctcgacgccg
     3121 cgtgccacca gaatgcgcag cgtggtctgt gccggcgcgg ccgacagttc cagcagcagc
     3181 ggaagatcgt ccggcgcgca ggcaaaaccc gcgtagcgct gcgtgcgcac cgtgacgtgg
     3241 tcttcttctg ccagcgcact ccagtgcgcg gtatccggct cgggcaggca cagcgtttcc
     3301 gggtagatgg cctctggcgt gaggtgacgt tcgcgcagcg gcgccagcca cgcttccacg
     3361 cgttcgcgcg ccgccacggc gaccgggaac tggccatcgg cctggcgcgg gcccagcgca
     3421 aagtgcagcg tgtcaacatc ctcggccagc tgttcttcca gcaggtaggg tgcggcctgc
     3481 agaactttcg caggcgcgcg cgcgggtact ttcacctgcg ccagccgcac gtctgcaccc
     3541 ggcaccagca gcacgatgcg gcgcgtacca gccagcgcca gcacctcggc cagcggcgcc
     3601 tcctgcgcgt ggaacgagcg cagcgcgttg gccggcgcga tgcagtaggc agtcacggcc
     3661 tcgggcgcgg tggagcgcag gcggatgtag agggtttcgc gcatctattc ggtgtcggtg
     3721 cttctgccga gcactacggg ggcgccctgg tcgggacggt agtagaaact atacaaggcc
     3781 agccgtccgc tgcctatgaa agtttccacc cgcgacatga agtagttgct cttgacgctg
     3841 atgccggcca ggcccatcgg cgtttccgga ccgatcatgg tctgcagcgc ggctgtgctg
     3901 tcggcgggct gctcctcgcg cgtggcgagg aatttttcca ggtcgggatt ggtctgcttc
     3961 accagcgagc gcagcaccgg ttccggtgcc gtattgatat tgaccggcgt gccgatctgc
     4021 ggcagcgcgg ccacgtagtt gcgcagcagg ccgtagagct tggcgtccat gcccttgatg
     4081 gcgcgcagct cgctcacgct ttccaggtag cggttggccg cgcggtaggg cggatccatg
     4141 ctcaggtatt cgctgtcttc cgcgccgtcc atgccggtag gcgtctggtc gggatcgatc
     4201 cagtcgcgga tcgctgcggc cagcggcctt gcctgcatgg catcggcgcc ctcgatgttc
     4261 tgcaccaggc gttcgaactg gtcgacatac ttggcataga ggttcggatc ggccacgccc
     4321 aggttgttga ggttgaagcg gccctgcagg tcgatcaccg cgccgcgcac gtagccttcg
     4381 tccaccggca gatagtcgac cggcttggcc cagtcatcct tgagcgaatc ggtcttgttg
     4441 tcctgcgcat cccgctgcag cacgctcttc agccaggact cgacgccgtc ggcataccac
     4501 caggcctttt ccgaatcctg catgttggcc gcgcggtgca ccgccatcat ggccgagctg
     4561 agcacggcgg cagcggcgat cgcggccagc gcgacgacca ggatcgcggt gatcagggca
     4621 acgccgcgct gggctttcat tgctggccct tgggcgtggt ggtcgaggtg accgtggtgg
     4681 tgggcgtcgt ggtcggcgcg ccgctggccg ctcccggcgt gtagccctgc ggcggtgtgt
     4741 ccagcccgat gcggaacagg aagcgcaggt caccccagtc cttcgtgcgc aggctcatct
     4801 cgaccgccat tggcggcggc ggggaggttt tctgctgagc gccgccggta ttatccggcg
     4861 gcggccatgt gtcactccat tcgcggctgg cgctgagata gcgcaggctc atgtcttcca
     4921 cctggtccag cagctcgctt tgtaccgcct tggaatcctg gccctggtcg agcacgcgga
     4981 aactttcacg catcagcttc ttgtcctgga gaatgtaatc tacgcgctcc aggccgggcc
     5041 gcggcagttc cgccgggttg cgccagccgc cgcgggtgaa ctccacgcgc gtgtcgtgat
     5101 cgccgcgcaa cgccggctgc gcatcgccgt aggcgtcgcg cgcagggcgc gtgcgcacct
     5161 gctgaaaatc atcgcgcagc cagcggtagg cacgctggaa ttccccggtg cgatccagcg
     5221 attcctccac ctggcgacgg cccttgagca ccgagttgag tccgccgtag gccatcagtg
     5281 ccaccagcgc aaaaatgccg atggccacta ccagttccag cagcgtgaag ccgggctgac
     5341 gtttcattgc cgccccgtgt cggagatgaa cgccgacatg ctggcgatgt cgagttcctt
     5401 gcccggcagc tgcacgcgga tgtcgatgcg gcgcaggtgt tcgtccggcg tcttcttcac
     5461 ctcgacgaac cagcgccact tgaaaccgcc catatcggca tcgccatcgc tcttgccctc
     5521 ggcgggccag ggcggctcga gctcgatctc ggtgagccgg tcatgcgcca cccaggtggc
     5581 cagcgtcttc tgccgcaggt agccggcgtt gctggcgtac atggccaggc cgcgcacgat
     5641 ggcgcccagc gccaccgcga tgatcgtcac cgcgaccagg atttcgatca gggtgaagcc
     5701 gcgcagtcgg cgatgagatt tcaaccctgc tcctcgtggc gctcaagctt gaaatggccg
     5761 agcgcatcgg cctcgatgtg gtaatacggc ttgtagccgc gcgcgcgcac gttgagcgcg
     5821 aacggcgtga cttcgccgct ggacagcagg aagaactgtg gttcgatctt cttctgcttg
     5881 tcggggtcct ggctgtcctg cgctggcgcc actgcgcggc cctcgacgaa caactcggtg
     5941 tagaacggat cgctcaccgc gcgcgtgcgc agcgggccgc cttcggcgta ctccttccag
     6001 ttgccgtcgt cgtcgcgcgc cagcagttcc atgcgctcgg gcgtataacg cacgccgatg
     6061 tcgatgccct tggtctcggc ttcttccgag gccagttgca ggatgcgctc catgcgcgcc
     6121 gcctcgttgt ccatgcggtc gtccatcacg cgatggccca tggagaagga gaagaaggtg
     6181 gcgatgatgc cgatgacgat ggtcaccacc atcatctcga tcagcgtgaa gccggactgg
     6241 cgcagcatgc gggtcggcct caccgacgtt ccgggccggg tcagttggtg ctggtcaggt
     6301 cccagctgcc gatatccgtg gcctcgccat cgccgcccgg cttgccgtcg ctgccgtagg
     6361 agaagatgtc gatttcgccc ttcacgcccg gcgacaggta ttgatacggg ttgccccacg
     6421 ggtccttggg cagcgacttg atgtagccgc cggccttgta attgtgcggc tgcgggtcgc
     6481 cgcccggctt gttcaccagc gcctccagcc cctgctgggt ggaggggtac gagaagttgt
     6541 ccagcttgta atactccagc gccgactcga gcgcgctgat gtcctgcttg gccttggcaa
     6601 tgcgcgcctt gaccggcgcg tccataatct tgggcacgac cacggcggcg agtatgccga
     6661 ggatcaccac cacgaccatg atctcgatca gcgtgaagcc ggctgcatgc cgggcgcgcg
     6721 cgaccgggcg aaaagaggtt ttgttcattg attgcccaga ctgaagcgga gggaaaaaag
     6781 ccttccggga tgatagcaaa agccccccaa agttccgttg cagtatgggc ggcgccggtg
     6841 gtgctcatgc tgctgctgct gggcaccgag ctgctcggcg actggggtcg cgagcacctg
     6901 cgctacgacc gcgcagcaat tgcagcaggg cagtggtggc gcttcatcac cggcaacttc
     6961 gtccacctgg gctggtatca ctgggcactg aacgaagccg gcatcgtcgt gctggtgctg
     7021 ctgtgcccgg agcagctctc gccgctggtg tggatgcggc gcgtgctgtt gctgtgcgtc
     7081 ggcatgagcg ctggcctgta ctggctggtg ccggggttgc cgacctatgt cggcatgtcc
     7141 ggcgtgatcc acggcttgtt cgtgctgggg ctgatgccac aggtgctgaa aaaggacctc
     7201 atcgcgctcg gctgcctgct gtacctgctg ggcaagctgg gctacgagct ttacgccggc
     7261 gcaccggttt ccgacgaaca tgccatcggt ggcagcgtgg tgacccaggc gcatttttat
     7321 ggttccatca gcgccctttt ctacggcctg gtgttccgca cttacacccg gcatgagacc
     7381 tggaggctga aatcgtgacg gcgccaagct acgcaatctt gtttcccgga cagggctcgc
     7441 aggccgtcgg catgctcggt gcgcacgacg cgccggtcgt tgcccggacg tttgccgagg
     7501 caggcgcagc gctcggctgg gatctggcgc agctggtgcg cggcggcccg gaagaagagt
     7561 tgaaccagac tgcgcgcacg cagccggcat tgctcgctgc aggtgtcgcg ctgtggcgcc
     7621 tgtgggacga gtcgggcttg cccaggcccg ccgcgctggc gggccacagc ctgggcgaat
     7681 acaccgcgct ggttgccgcg ggcgcgctcg atttcgccga cgcgctgcgg ctggtcgaac
     7741 tgcgcgggca gctgatgcag gccgcggtgc cggccggcac cggcggcatg gcggcggtga
     7801 tcggcctcga tgatgcgaag gtcgcggacc tgtgcaaggc gtatcccggc agcgaagtgc
     7861 tggaaccggt gaactacaac gcgccggggc aggtggtggt ggccggccag gcggcggcgc
     7921 tggactggct tgcagccaac ggcaaggcgc acggtgcgcg catggtgatg cgcctgccgg
     7981 tgtcggtgcc atcgcactgc tcgctgatgc gcgacgcggc gcagaagttg gccgacaagc
     8041 tcgccgccac ttcaattcac gcgcccgcaa taccggtcta ccacaatctc gacgccgcgc
     8101 cgcacagcga gcccgcctcg atccgccggg cgctcaccga gcagctgtac cgtccggtgc
     8161 tgtggacgtc gacgatccag aaactggtgg cgtcgggcat cggcacgttc gtcgagtgcg
     8221 gccctggcaa ggtgctcacg ggtttgacca agcgcattgc cgacaagtcg gcgcgttatg
     8281 tttcgctgga agatccggcg gggctgcagc aggcgcttgc gtggctgaaa acggcttgac
     8341 cgcaccccgg ttctttctgt tataaaatat cattcaatta tagaaatata ctatatttaa
     8401 taacatcgtt ttggcaggtg cggtcaacgt gttttggagg tcgagatgaa cttgcagggt
     8461 gaaattgcgc tggtgactgg cgccagccgc ggcatcggcc gcgccatcgc cgaacgcctg
     8521 gcgcgcgatg gcgcgcaggt gactggcacc gcgacgaccg acgaaggcgc tgccgcgatc
     8581 acgcaatatc tcgcgccgat gggcggccat ggcatcaggc tcgacgtgcg cgacgccgcg
     8641 gccatcgaag cggcgctggc gcaggccggt gcggtgtcga tcctggtgaa caatgccggc
     8701 gtcacccgcg atacgctgct gctgcgcatg aaggatgagg attgggagca ggtgctgcag
     8761 accgatctca cctcggtgtt ccgcctgtcg cgcgcggtgc tgcgcggcat gatgaaggcg
     8821 cgccacggcc gcatcgtcaa catcggctcg gtggtgggcg cgatgggcaa ccccggccag
     8881 gccaattact gcgcggccaa ggccggtctg gtcggatttt ccaaatcgct ggcgcaggaa
     8941 atcggttcgc gcggcatcac cgtcaacgtg gtggctccgg gcttcattga caccgacatg
     9001 accaaagggc tgggcgagga cacgcgcaag gcgctgatcg accgcgtgcc ggtgcagcgc
     9061 ctgggggcgc cggacgatat cgccgcggcc gtggcatttc tggcctcgcg cgacgccggc
     9121 tacatcaccg gcgagacgct gcacgtcaac ggcggcatgt acatgaattg agcatcagac
     9181 gaactttgcg gtggataagc gggccgaaaa tgcaagactc ggccgcgctg tcgggaaatt
     9241 ccggtttcac ttaaaatagg ccggcttcac acattaaatc gattattcac ggagctacat
     9301 ccatgagcag cttggaagaa aaggtcaaga agatcatcgc ggaacagctg tccgtcagcg
     9361 aggatcagat cacccccgag gcttcgttcg tcgaagacct tggcgccgac tcgctcgata
     9421 cggtggagct ggtgatggcg ctggaagaag aattcgaaat cgacattccg gacgaggaag
     9481 ccgagaagat cgtgacgttc caggacgtca tcaactacat caagtcgcac gccacccagc
     9541 agggctgagc ggaaaaatcc ccgcagtacc cacaatccag aaggagcagg catgagccgc
     9601 aggcgcgtgg tcgtcaccgg catgggcatc gtttcgcccg tcggttccac cgtcgacagc
     9661 gcctgggcca atatcctggc cggcaggtcc ggcatcacgc cgatcacgcg gtacgacatc
     9721 tccagcttcc cgacgcggtt tgccggccag gtggccgact tccgcgccga ggagtggatg
     9781 aatcccaagg atgtgcgccg cgtggacgaa ttcatcgtct atggcgtggc cgcggccaag
     9841 caggcgatga aggatagcgg gctggaagtg accgacgcca accgcgagcg catcggtgtt
     9901 tgcgtgggct cgggcatcgg cggcatcggc accattgaag agcagtgcat gctgctcaac
     9961 aaccagggtg tcaagcgcgt ctcgccgttc ttcgtgccgt cgtcgatcat caacatgatc
    10021 tccggccaca tctcgatcga cctgggcatc accgggccga acctggcgac ggtttccgcc
    10081 tgcaccaccg gcgcccacgg catcggcatc ggtgcgcgcc tgatcgccta cggcgatgcc
    10141 gacgtgttca tcggcggtgg cgctgaggcg ggttcggctc cggccggcat ggcgggtttc
    10201 tgccaggcgc gtgcgctgtc gacgcgcaac gacgatccgc agcgcgccag ccgcccgtgg
    10261 gacaaggacc gcgatggttt cgtgctgggt gacggcgccg gcgttctggt gctggaggaa
    10321 tacgaacacg cgaagaagcg cggcgcgcgt atctatgccg agctgatcgg tttcggcatg
    10381 tcgggcgatg cacaccatat caccgctccg cccgacgatg gcgccggcgc gcgccgggcc
    10441 atggtggcgg cgctcaagga cgccgggatc aatgccgggc aggtcgacta catcaacgcg
    10501 cacggtacgt cgacgccgct gggagacgtg gctgaaacca tcgccatcaa agcggcattc
    10561 ggcgcgcacg cgcacaaggt tgcggtgagc tcaaccaagt cgatgaccgg gcacctgctc
    10621 ggcgcggctg gcggcgtcga ggcgatcttc tcggtgctgg cactgcgtga ccaggtgatt
    10681 ccgcccacca tcaacctgga aaccccgggc gaaggctgcg acctggacta tgttcccggg
    10741 actgcgcggc aggccaagct gtccatcgcg atttccaact cgttcggctt cggcggtacc
    10801 aacggcacgc tcgcgttcca gacgctgaac tgagttaagg cggcgcatga gctttcgcgc
    10861 cgaagtgact ggtcagattg acctttttaa tctgcatagg cagcagccag aagtcttccc
    10921 attcctcctg caaagcctcg gcgccggcag cggccgctac gacctgctgt ttgccttccc
    10981 aggtgacacg atcgaagggc cggaattctt tgccgggctc aaccagcagg cggccgcgaa
    11041 gcgctgcgca ccgcaggacg aactgccgtt ctgcggtggc tggttcctgc tcctgggcta
    11101 cgaggcggcg cgctggattg aaccgcgcct gcgactgccg gcgtcgccgc attcgttgcc
    11161 ggacgcgctg gcggtgcgct gtaccgccgt cgcggtgtat gaccatcagc gccggagtac
    11221 gacggcttat gctgaatcat cgcaggcgga actggatgaa gtcgtcgctg cgctccgaag
    11281 caatcttggt tcgccggcgt acgcgcccgg gaacgggctg cagctccatg tccaggctga
    11341 tgtcgatggg gaattcgagg caggcgtgcg ctcgatactc gaatacctga aggctggaga
    11401 tgtcttccag gtgaacctgt cgcgggcctg gcgcgcgtgg atggatcagc gatccagtta
    11461 tctcgatctg tatgcccggc tgcgccacgc caatcctgca ccgttcgccg gcttggcgcg
    11521 atggggcgat gccgtcctgc tcagctcttc acccgagcgg ctggtcgaaa tccgcgacgg
    11581 ctgggtgcag acgcggccca tcgccggtac gcgtccgcgt ggcaatggca caggcgagga
    11641 tgagcaattg agggccgcac tcatcgccag cgtcaaggag cgcgccgagc acatcatgct
    11701 gatcgaccta gaacgcaacg atctcgggcg cgtgtgccag ccgggcagcg tcgaggtcag
    11761 cgagttgatg accatcgaga gctatgcgca cgtgcaccac atcgtgtcca acgtgcgcgg
    11821 gcgcttgcgg gctgaggcgg gtccaggcga cgtgctgcgc gcggtatttc ccggaggcac
    11881 catcaccggc tgcccgaaag tccgggccat ggaaatcatc gcagaacttg aatgcgtcgg
    11941 tcgcggcccg tataccggcg ccatgggcta cctgtcgcgc tgcggccgca tggataccaa
    12001 cattctgatc cgctcgctgc tgtgcgaaag cgacacgctg accttccgca ccggcgccgg
    12061 catcgtcgcc gattccgatc cgcaggcgga actggccgaa acgcgtgcca aggcgcgcgg
    12121 cctgttactg gcgctggggg cgcagccgtg agcgcgttgt acaacggcaa gccggccgag
    12181 tcggttccgc tgcacagccg cgggctgaac tatggcgatg gcgttttccg cacgctgctg
    12241 gtgacggacg ggcggatcca cgattgggcg ctgcaatacg ccacgctggc gcgcgatgca
    12301 gcccgcttgt cgctgcagct gcccgaggag ggactgctgc gttcggagtg cgatttccta
    12361 gcggcagacc ggcgtctcgc agcgatgaaa gtcatgctgg tccgtgcggg cgaacggcgc
    12421 ggctacgcac cggagacttc tacctgcgaa cgcctggtct cggtgcatga cctgccccga
    12481 tatccatcgc ggcactggtc gcgcggtgtg gcggtgatcg ggtcgacggt gcagctggcc
    12541 caacagcctt tgctcgcagg catcaagcat ctcaaccgcc ttgagcaggt gctggccagc
    12601 cgtgactggc cgcaggacat ttccgaagct ctcatgtgcg atcatgccgg ccgcgtcgcc
    12661 ggcggcacgc gcagcaacct gttctgggtc aggcagggca ggctgtatac gccggacctg
    12721 agccgatcag gcgtcgatgg gatgatgaaa tccaaggtga cagcgcttgc ccgggcgcat
    12781 tcgatggaac ttcgcacggt ggacgcagac tgggccgagc ttgaacaagc cgatgaaata
    12841 ttcctcacca actcgctcgt cggcatctgg ccggtgcgtg cgctggaagg ccggatgctg
    12901 gacgcgcccg gcgtggtgac cgcccggttg agcgcgctgc tgcaccatcc attgccgatc
    12961 gcatgagacg ttttctcgcc ggaatcctgc tggttccgct gctgctggcg gcggccgcgc
    13021 tgctggcctg gcgcgacgcg cagggcacgc tggcggcgcc gttgcccatc accgaaaaat
    13081 tcatctacga aatcccggtc ggagcgcctt tgcagcaggt gatcgccgac ctgcgccagc
    13141 gcggcgtcgt gctggatcgg cgcctgggcc tgtacctgcg catctatgcc gaggtgaaga
    13201 agctcaacct gcgtgccggc gaatacgaac tgacgcccgg catctcggcc atcgaactgc
    13261 tgacgacgtt cgcctcgggc aagaccctgc tgcacgagtt gcgcatcgtc gaaggctgga
    13321 cctttgcgca ggccatgaag gtgattgctg ccgacccgga tcttgcccac agcctcgagg
    13381 gcaaatcacc cgagcaggtg atggcccagc tgggctacac cgacaccttc gccgaagggc
    13441 gcctgttccc ggatacctac cgcttcccca aggggctcag cgatatcacg ttcctgcggc
    13501 gcgcggcagc gaccatggat gcgatgctgc agaaggagtg ggacggccgc gatgccgatc
    13561 tgccatatgc ctcgccggcc gacgcattga tcatggcctc gatcgtcgag aaggaaaccg
    13621 ggcaggccag cgagcggccg gaagtggcgg gggtgttcgt caaccgcctg cgcagcggca
    13681 tgaagctgca gaccgatccc accatcatct atggactggg accggcgttc gacggcaacc
    13741 tgcgcaagat tgacctggaa accgacggcc catacaacac ctatacccgc gccggattgc
    13801 cgccgacgcc gatctgtctg ccggggcgtg cctcgctgca cgccgttctg caccccgcgc
    13861 agaccaaggc cctgtatttc gtgtcgcgtg gcgatggcac gcatgtattc tcagaaacct
    13921 atgacgcaca cagtgccgcc gtacagaaat tccagatcgc gccccatgcg gcccatggca
    13981 agaaataagc gcggccgttt catcacgctg gaaggcggcg agggcgcagg caagtcgacc
    14041 aacgcgcgct tcatccgcga ctggctgacg gcgcgcgggc gcgaagtcgt cctgacccgc
    14101 gagcccgggg ggtcgccgat ggccgaggcc atccgcgagg tcgttctgcg cgactggaag
    14161 gagtcgatgg cgccggacac cgagctgctg ctgatgttcg ccgctcgcgc ctcgcatctg
    14221 cacagcgtca tcctgcccgc gcttgcggcg ggcaaggacg tggtctgcga ccgtttcatc
    14281 gactccacct acgcttacca gggcgccggc aagcagatgc cggtggcgag aatcaagctg
    14341 gtggaccgca tggtgctggg tacttttgcg cccgatctca cattgctgtt cgaccttccg
    14401 ccggaagaag gtctgaagcg ggcccacgcg cgtggagaaa agaaccgttt tgaggcggaa
    14461 tcgctggcct acatgcggcg cgtacgcgcc tgcttcctgc agcgcgcgcg cagcgcgccg
    14521 ggccgctacg ccgtcatcag ggccaaccgg ccgttgcccg cggtacagaa gaccgttgcc
    14581 cgcgtgctgg agcagaagct atgagcgtca tacccaagcc gttgccctgg cagcaggaac
    14641 tatgggtgga ggtgactgcg ctggcactgc aggatcgtct tggccacgca ctgttgctga
    14701 gcgggccagc cggcgtgggc aagcgggcct tcgccaaggc gctcaccgcg ttcctgctgt
    14761 gtgagcagcg cagcggctac gcctgcggca ggtgccgcag ctgcgagcag ctcgccgccg
    14821 gcgcgcatcc caattcaatc gtgctggcgt tggaagtcga cgacaagacg ggcaagcaga
    14881 agcgcgatat ttcagtggag caggtgcgcg aattcagcga acgcctgcag ctcacttcgc
    14941 actacggcca ggccaaggtg gcgaccatcg acccggccga cgcgctgaac gctgcgggcg
    15001 tgaatgcctt gctcaagacc atcgaggagc cgtccggcca cacgcatgtg ctgctgattt
    15061 ccgagcgcgc catggcgttg ccggcgacgc tgcgcagccg ttgccagcga ctgcagttcg
    15121 cgccgccggc gcagcaggag gctatggcct ggctgcacga acatcatcca gaagtgggtg
    15181 aacagacgct cgcactggcg cacggcgcgc ccttgcgcgc ggcggaactc gcgcagtccg
    15241 gcctggtcga gcgctatgcc agctgggaaa agggcatgag cgatcttgct gctggacgtg
    15301 gcgatccgct ggcgctcgcg gcggccattg ccaaggaaaa agatgacgtt cctgcctatt
    15361 tcagctggct gcagctgtgg ctggctggac tgctgcgcgg cgcggtcggc gcggcgccga
    15421 aaaagctggc gctgcccgcc ccggcactgc agcagatgat gcgtgaggcc atcgacgcgc
    15481 agcggcggct cggcggcaac gccgttcccc agctcctgct cgagtcggtc atcgtgctgt
    15541 ggtggcgcct tgcgcgcccg gtaaaggccg cgtgagtttt tgaggcgtgt gcgggcagcg
    15601 gtgtaagatc gccgtatcat tggcttgctt gcgacggaac tgatcatggc cacgcctccg
    15661 cgcccctcgg gcggcgcaca acccggcatc ctgacgctca acatcaagga caagagcgcg
    15721 ctgtacgtcg cttacatgcc cttcgtgaaa aacggcggtc tgttcattcc gaccaacaag
    15781 gaataccgcc tgggcgatga agtgttcatc ctgctcac
//
LOCUS       DASZZC010000458         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_488582,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000458 DASZZC010000000
VERSION     DASZZC010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488582"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2873)
                     /locus_tag="VHE37_09735"
     CDS             complement(<1..2873)
                     /locus_tag="VHE37_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT35855.1"
                     /translation="MNLTEISLKRPVTVTMFFVCVAVIGVIGATRLPLESCPDIQFPG
                     LQVTVPYRNSTPEEVERHITRPVEEALATLSGVKHIYSESREDGAEINLEFDWGQDLA
                     TKGVEARDKVDALRPSLPQEVERVLIQKFNAGDQAMLTLRISSGGRDLSNAYDMLNRN
                     LKLRLNRIEGVSRVDLYGVEQKQIRVDLSAEQVAAHGIDLGKLADTLKNANFALTAGN
                     LVDAGSRYFVKPEGRFKSLDDVRNFVVNDQGLRLGDIAGIVYSDPVLTYGRHLNQRYA
                     IGLDVYKETGANLVEVSDRAKAELEEIRKLPEMAGINVITFNDSADDVRRSLHDLEEA
                     GAIGALLSVLVLWFFMRDLTMTLIVTLSVPLSLLMTLAAMFFLHYTINVLTLMGLMLS
                     IGMLVDNSVVVTESIFKERAAGADTSLPPPQLSGAKGEAIGAIPGAPAPAPAPALRGT
                     VSQARTSAEGATLAGVKRVGLAVLMGTLATSIVFLPNIFGRQNDITVYLSHVAVTICC
                     SLAASLLVAITLIPQLTTRLAARHRGSTWLEPYTQRYMNLLRWTLHHPYRMTLFILLL
                     LGSVAIPVSQVKNDFFPNSSDKRLVLQYNLNGVYRLAKVEEAANTIENWLAAHKAEFE
                     IDSVYTYYSIERAQTLIGLKDPKTTVRTKSGDQIKEEIRKGLPKIAIGDPSFDNQRGG
                     GQKLSVQVYGEVSDELHGVAKDVARVLRGIPGLTDVRIEASAADWEVRVRVDRDRARQ
                     NGLSSQQVAEIVAGAMRGTALKPYRTPTGEIEMVLEFSKEDRLDLDALRALPIITPTG
                     ARVALATVADLSIGDVPGIIKREDRHVSLNVSFGTAKGVTADDAKKRATQALNELQFP
                     SGYGWAFGRAFNEEDEDMGVMMVNMALALACIYIVMAALFESVLAPTAIITGIVFSFV
                     GVYWFFFLTHTAFSFMAMIGMLVLMGVVVNNGIVLIERVHQLR"
     gene            complement(2870..>3757)
                     /locus_tag="VHE37_09740"
     CDS             complement(2870..>3757)
                     /locus_tag="VHE37_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT35856.1"
                     /translation="VATRYRMADREIDVLVRGAEADRNSVAAVQGMVINPENPRPLSV
                     SAVADVRLRNGPSEIRRISQQRVIVLSAGVAEGNLGDAVADLKGIIGTVPMPQGISAL
                     VAGQSQEMEVSFRSLQLALALAVFMVYLVMASQFESLRHPFVIMFTIPLAVIGAVFAL
                     FVTRSVINVVALIGMVMLIGIVVKNGIVLIDLVNRLRAQGLSRRDAVLAAGPVRLRPI
                     LMTTLTAVLGLVPMALGAGGGAEVRAPMAITVIGGLTISTLLTLVVIPVVYTLIDRDD
                     HVPQPAAQPAGGDVLGFQK"
BASE COUNT          635 a         1250 c         1254 g          618 t
ORIGIN      
        1 tcgcgcagct ggtgcacgcg ctcgatcagc acgatgccgt tgttcaccac cacgcccatc
       61 agcaccagca tgccgatcat ggccatgaac gagaacgcgg tgtgggtgag gaagaagaac
      121 cagtacacgc cgacgaacga gaacacgatg ccggtgatga tggcggtcgg cgccagcacg
      181 ctctcgaaca gcgcggccat gacgatgtag atgcaggcca gcgccagcgc catgttgacc
      241 atcatcacgc ccatgtcctc gtcctcctcg ttgaaggcgc ggccgaaggc ccagccgtag
      301 ccggaaggaa actgcagttc gttgagcgcc tgcgtggcgc gcttcttggc gtcgtccgcg
      361 gtcacgccct tggcggtgcc gaagctgacg ttgagcgaca cgtggcgatc ctcgcgcttg
      421 atgatgccgg gcacatcgcc gatggacagg tcggccaccg tggccagcgc cacgcgcgcg
      481 ccggtgggcg tgatgatcgg cagcgcgcgc agcgcgtcga gatccagccg gtcttccttg
      541 ctgaactcga gcaccatctc gatctcgccg gtgggcgtgc ggtacggctt gagcgcggtg
      601 ccgcgcatgg caccggccac gatctccgcc acctgctggc tcgacaggcc gttctgccgc
      661 gcgcggtcgc ggtccacgcg cacgcgcact tcccagtcgg cggcgctggc ttcgatgcgc
      721 acgtcggtca ggccggggat gccgcgcagc acgcgcgcca cgtccttggc cacgccatgc
      781 agctcgtcgg aaacctcgcc gtacacctgc accgacagct tctgtccacc gccgcgctgg
      841 ttgtcgaacg acggatcgcc gatggcgatc ttgggcaggc ccttgcggat ctcctccttg
      901 atctggtcgc cggacttggt gcgtaccgtg gtcttggggt ccttcagccc gatcagcgtc
      961 tgtgcgcgct cgatcgagta gtaggtatac accgagtcga tctcgaactc ggccttgtgc
     1021 gcggccagcc agttctcgat ggtgttggcc gcctcttcca ccttggccag gcggtacacg
     1081 ccgttgaggt tgtactgcag caccaggcgc ttgtcgctgc tgttggggaa gaaatcgttc
     1141 ttcacctggc tgaccgggat cgccaccgag ccgagcagca gcaggatgaa cagcgtcatc
     1201 cggtacgggt ggtgcagggt ccagcgcagc aggttcatgt agcgctgggt gtatggctcc
     1261 agccaggtgc tgccgcggtg gcgcgcggcc agccgcgtgg tcagctgcgg gatcagcgtg
     1321 attgccacca gcagcgaagc ggccagcgag cagcagatgg tcaccgccac gtgcgacagg
     1381 tacacggtga tgtcgttctg ccggccgaag atgttgggca ggaacacgat cgaggtggcc
     1441 agcgtgccca tcagcaccgc caggcccacg cgcttcacgc cggccagcgt ggcgccttcc
     1501 gcggaggtgc gcgcctgcga cacggtgccg cgaagcgcgg gtgcgggagc aggcgcaggg
     1561 gcgccgggaa tcgcgccaat ggcctcaccc tttgcgccag acaactgggg tggtggaagt
     1621 gaagtatcgg cgccggcggc gcgctccttg aagatgctct cggtcaccac caccgagttg
     1681 tccaccagca tgccgatcga cagcatcagg cccatcagcg tgagcacgtt gatggtgtag
     1741 tgcaggaaga acatcgcggc cagcgtcatc agcagcgaca gcggcaccga cagcgtgacg
     1801 atcagcgtca tggtcaggtc gcgcatgaag aaccacagca ccagcaccga cagcagcgcg
     1861 ccgatggcgc cggcttcctc caggtcgtgc agcgagcggc gcacatcgtc ggccgaatcg
     1921 ttgaaggtga tgacgttgat gccggccatc tccggcagct tgcggatttc ctccagctcg
     1981 gccttggcgc ggtcggacac ctcgaccaga ttggcgccgg tctccttgta gacgtccagg
     2041 ccgatcgcat aacgctggtt gagatggcgg ccgtaggtca gcaccgggtc gctgtaaacg
     2101 atcccggcga tgtcacccag gcgcaggccc tggtcattca ccacgaagtt gcgcacgtcg
     2161 tccaggcttt tgaagcggcc ctccggcttg acgaagtagc ggctgccggc atcgaccagg
     2221 ttgccggcgg tgagcgcgaa gttggcgttc ttcagcgtat cggccagctt gcccaggtcg
     2281 atgccgtgcg cggcaacctg ctcggccgac aggtcgacgc ggatctgctt ctgctcgacg
     2341 ccgtacaggt ccacgcgcga cacgccctcg atgcggttga gccgcagctt gagattgcgg
     2401 ttgagcatgt cgtaggcgtt cgacaggtcg cggccgccgg aggagatgcg cagcgtcagc
     2461 atcgcctggt cgccggcgtt gaacttctgg atcagcacgc gctcgacttc ctgcggcaat
     2521 gaagggcgca gcgcatcgac cttgtcgcgc gcctccacgc ccttggtcgc caggtcctgg
     2581 ccccaatcga attccaggtt gatctcggcg ccgtcctcgc gcgattccga gtagatgtgc
     2641 ttgacgcccg acagcgtggc cagcgcttcc tccaccggcc gcgtgatgtg gcgctccact
     2701 tcctccggcg tcgagttgcg gtacggcacc gtcacctgca ggccgggaaa ctggatgtcg
     2761 ggacacgact ccagcggcag gcgcgtggcg ccgatcacgc cgatcaccgc cacgcagacg
     2821 aagaacatgg tcaccgtgac cgggcgcttg agcgagatct cggtgaggtt catttctgga
     2881 atccaagcac gtcgccgcca gccggctgcg ctgccggttg cggcacgtgg tcgtcgcggt
     2941 cgatcagcgt gtacaccacc gggatcacca ccagcgtgag cagggtggag atggtgaggc
     3001 cgccgatcac ggtgatcgcc atcggtgcgc gcacctcggc gccgccgccc gcacccagcg
     3061 ccatcggcac caggccgagc acggcggtga gcgtggtcat caggatcggg cgcaggcgca
     3121 ccgggccggc ggcgaggacg gcgtcgcggc gggagagacc ctgcgcgcgc aggcggttga
     3181 ccaggtcgat cagcacgatg ccgttcttca ccacgatgcc gatcagcatg accatgccga
     3241 tcagcgccac cacgttgatc accgagcggg tgacgaacag cgcgaacacc gcgccgatca
     3301 ccgccagcgg gatggtgaac atgatgacga agggatggcg cagcgattcg aactgcgagg
     3361 ccatgaccag gtagaccatg aacaccgcca gcgccagcgc cagctgcagc gagcggaacg
     3421 acacttccat ttcctggctc tggccggcga ccagcgcgct gatgccctgc ggcatcggca
     3481 ccgtgccgat gattcccttg aggtcggcca ctgcatcacc gagattgccc tcggccaccc
     3541 cggccgacag cacgatcacg cgctgctggc tgatgcggcg gatctcggac gggccgttgc
     3601 gcagcctgac gtcggccacc gcggaaaccg acagcggacg cggattctcc gggttgatca
     3661 ccatgccctg caccgcggcc acgctgttgc ggtcagcctc ggcaccacgc accagcacat
     3721 caatctcgcg gtcggccatg cggtagcgcg tggccac
//
LOCUS       DASZZC010000459         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_98529,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000459 DASZZC010000000
VERSION     DASZZC010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98529"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VHE37_09745"
     CDS             complement(<1..391)
                     /locus_tag="VHE37_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT35857.1"
                     /translation="MRTIFAITLMNLRSIPARLGSSLVIVVGIAFVVAVLVSMLSMSR
                     GFDRTLRGTGNDGRVLFLSTGVLAELSSYITPDILPLVEGLPGLATDAAGRPLVSPEL
                     VVITELRKGDGPKAASVNVALRGIGAAG"
     gene            complement(395..1555)
                     /locus_tag="VHE37_09750"
     CDS             complement(395..1555)
                     /locus_tag="VHE37_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT35858.1"
                     /translation="MKYFYLIWAQLWRKKLRTWLTVMSLVTAFLLLGLLQAADALFNG
                     SGVNLSAPILIAQARVSFTNPLPMRLLPQIEAIPGVEAVSHSQFFGGVYQDVNNRVIQ
                     FAVNPERWLKVFPECVLPPAQELAWKQNAAGIIAGQAVADRFHWKVGDRIPLVSQIFP
                     KKDGSRAWEFDLVGIFGDRSKESCPRLNNMYISYTGFDEARQFGRGMAGIYVIRIHNP
                     DDAQRIAAQVDAMTENSPDETKTQTERDFTLNFAKQVGNLSLIFNLILFAVFFSILML
                     TGNTMWQAVRERVPELAVLKTIGFTDGEVVGLVLAESLLMCVLGGTVGMLAASGVLHV
                     LARLPTGFPPPQNSVALWVWAVVVMLLLGLIIGVLPALRAKRLSIVDALAGR"
     gene            complement(1804..>2350)
                     /locus_tag="VHE37_09755"
     CDS             complement(1804..>2350)
                     /locus_tag="VHE37_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT35859.1"
                     /translation="TTLLNLIGGLDRPTGGSVSVGGERIDQLSSGQLAKWRSQHVGFV
                     FQFYNLLPVLSAERNVELPLLLTSLNKEQRAKHVATALQIVGLADRAKHKPSELSGGQ
                     AQRVAIARALVADPTLLVCDEPTGDLDRKTADEVLHLLQELNQKHGKTIVMVTHDPKA
                     AEYATHRLYLDKGALVDGMAH"
BASE COUNT          406 a          788 c          787 g          369 t
ORIGIN      
        1 ggcccgccgc gccgatgccg cgcagcgcca cgttgacgct ggccgccttc ggcccatcgc
       61 ccttgcgcag ctcggtgatc accaccagtt ccggcgacac cagcggccgg ccggcggcat
      121 cggtggccag gcccggcagg ccctcgacca gcggcaggat gtcgggcgtg atgtagctgg
      181 acagttcggc cagcacgccg gtggacagga acagcacgcg gccatcgttg ccggtgccgc
      241 gcagcgtgcg gtcgaaaccc cggctcatcg acagcatcga caccagcacg gcgacgacga
      301 aggcgatgcc gaccacgatg accagcgacg agcccagccg cgcgggaatg ctgcgcaggt
      361 tcatcagggt gatggcgaag atcgttctca cgctttatct ccccgccagc gcgtcgacga
      421 tggacagccg cttggcgcgc agcgccggca gcacgccgat gatcaggccc agcagcagca
      481 tcaccaccac cgcccacacc cacagcgcca cgctgttctg cggcggcgga aaaccggtgg
      541 gcaggcgcgc cagcacgtgc agcacaccgc tggcggcgag catgcccacg gtgccgccga
      601 gcacgcacat cagcagcgac tcggccagca ccaggccgac cacttcgccg tcggtaaatc
      661 caatcgtctt gagcaccgcc agttcgggaa cgcgctcgcg caccgcctgc cacatggtgt
      721 tgccggtgag catgaggatg gagaagaaca ccgcgaacag gatcaggttg aagatcagcg
      781 acaggttgcc gacctgcttg gcgaagttga gcgtgaagtc gcgctcggtc tgcgtcttgg
      841 tctcgtcggg cgagttctcg gtcatcgcat cgacctgcgc ggcaatgcgc tgcgcgtcat
      901 cgggattgtg gatgcggatg acgtagatgc cggccatgcc gcggccgaac tggcgcgcct
      961 cgtcgaagcc ggtgtacgag atgtacatgt tgttcagccg cgggcacgac tccttgctgc
     1021 ggtcgccgaa gatgccgacc aggtcgaatt cccaggcgcg cgagccgtcc ttcttcggaa
     1081 agatctggct gaccagcgga atgcggtcac cgaccttcca gtggaagcgg tcggccaccg
     1141 cctggccggc gatgatgccc gcggcgttct gcttccatgc cagttcctgc gccggcggca
     1201 gcacgcattc ggggaacacc ttgagccagc gttcaggatt gaccgcgaac tggatcacgc
     1261 ggttgttcac gtcctggtac acgccgccga agaactgcga gtgcgacacc gcttccacgc
     1321 cgggaatcgc ctcgatctgc ggcagcagcc gcatcggcag cgggttggtg aacgacacgc
     1381 gcgcctgcgc gatcaggatc ggcgccgaaa gattcacgcc gctgccgttg aacagggcgt
     1441 ccgccgcctg cagcaggccc agcagcagga aggcggtgac cagcgacatc accgtcagcc
     1501 aggtgcgcag cttcttgcgc cagagctgcg cccagatcag gtagaaatat ttcatggcgc
     1561 agccacgtct gcgttaactc ctgaaagcgt cgcgagcgaa ggcagtttgc gcgaggctgg
     1621 agcgcagcga gcggagcata tgtggcgcgt gagccggagt ggcgcgcagc gccgaatgag
     1681 cgccgagcgc gccatacggc catgtccgca cccgcactcg ctgcgctcgt gacaccgtga
     1741 catggccgta tatgtgagca ttgcgagcac caccctcgcg caatctgccg agcgcagcag
     1801 ctttcagtgc gccatcccgt cgaccagcgc acccttgtcc agatacagcc ggtgcgttgc
     1861 gtactcggca gccttcgggt catgcgtgac catgacgatg gtcttgccgt gcttctggtt
     1921 cagctcctgc agcaggtgca gcacttcgtc ggcggtcttg cggtccaggt cgccagtggg
     1981 ctcgtcgcac accagcagcg tggggtcggc caccagcgcg cgggcgatgg ccacgcgctg
     2041 cgcctggccg ccggagagtt cgctcggctt gtgcttggcg cgatcggcca ggccgacgat
     2101 ctgcagcgcg gtggccacgt gcttggcgcg ctgctccttg ttgaggctgg tcagcagcag
     2161 cggcagttcc acgttgcgct cggccgacag caccggcagc aggttgtaga actggaagac
     2221 gaagcccacg tgctgcgaac gccacttggc cagctgaccg ctcgacagct ggtcgatgcg
     2281 ctcgccgccg acgctgaccg agccgccggt ggggcggtcg agcccgccga tgagattgag
     2341 cagcgtggtc
//
LOCUS       DASZZC010000460         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1192369,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000460 DASZZC010000000
VERSION     DASZZC010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192369"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VHE37_09760"
     CDS             <1..1906
                     /locus_tag="VHE37_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HVT35860.1"
                     /translation="IAVMLGTGGVVVFNTRVINPWGTVLDSERWAAEYERQLFPYKSL
                     TPPRITDVKLDIDIDSSVPRATTRGTFTVENKSAQPISDVPVFWVHRQEKGTWLGTSA
                     TPDLELKSLEIPGARLVKEIPELHFRIYRFDTPLQPGQRTEIRFKTVREQIGFRNNNN
                     ESRIVENGTFLTSHEITPVLGVHNLFALQERTARRHYGLPDELRPPKLDDNGPRGNNY
                     FRPDSDWVNADLTIHVAADQTVVAPGERVEDTVAGSRRTTHFRTTAPILHFFTIQSAR
                     YAERKEQWKDVALEVYYHPGHPYNIDRMLRAMRAALEYYSTNFSPYQFKSLRIAEFPA
                     YRDFAQAYPGTIAYGEASGFTNGTPRNYDGVTRVTAHETAHQWWAHQVIGADMQGQTV
                     LSETLAEYSALMVMEKINGPDEINRFLRASLDSYLRARGSDITGEVPLELVEEQPHIR
                     YTKGGMVMYLLKDLMGEDAVNRALRSLIQDYAFKGPPYPTAHDLTRRLRAQAPPELLP
                     LIADLFEQITTYDFSVVNARSAVRPDGKWDVTIELDAHKYYADGKGAQREAPLDTLID
                     VGVFTAEPGKPGFGAGSVLHLAHHAVRSGRQTLTLVVDREPKFVGADPYSKYIDIVPN
                     DNILALDGRQ"
     gene            1927..>3079
                     /locus_tag="VHE37_09765"
     CDS             1927..>3079
                     /locus_tag="VHE37_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT35861.1"
                     /translation="MTNPFLARAASSIFRYGGSFADFVPVRAEGSFLYDSAGRRVLDF
                     TSGQMSAILGHSHPEITATVCEWAGKLAHLHSSMLSEPVVGLAEALVKLAPGLDRVLL
                     LTTGAESNEAALKMARLATRGYEVVGFGQSWHGMTGGAAAATYSAGRKGYGPMGVGNL
                     AIMAPNPYRPTFTANGNYDWRAELDYGFELVDRQSTGSLAAFIAEPILSSGGILELPE
                     GYLAALKEKCRERGMMLVLDEAQTGIGRTGNTFAFERDGVVPDILTLSKTIGAGLPLA
                     AVMASAATEALCHERGFLFYTTHVSDPLPAAIGLTVLKVLARENLTARARAAGARLKA
                     GLQAMMAEFDCVGDVRGRGLLLGMEIVKDKQSKAAAPELGSRITEHAMRF"
BASE COUNT          552 a         1051 c          992 g          484 t
ORIGIN      
        1 gatcgccgtc atgctcggga ctggcggcgt cgtggtcttc aacacgcgcg tcatcaaccc
       61 gtggggcacc gtgctcgaca gcgagcgttg ggcggcggag tacgagcggc agttgttccc
      121 ctacaagtcg ctgacgccgc cgcgcatcac cgatgtgaag ctcgacatcg acattgacag
      181 cagcgtcccg cgcgcgacga cgcgcggcac cttcaccgtg gagaacaagt cggcgcagcc
      241 gatttccgac gtgccggtgt tctgggtcca ccgccaggag aagggcacct ggttgggcac
      301 cagcgcgaca cctgatctgg agctgaagtc gctggagatc ccgggcgccc gcctggtgaa
      361 ggagatcccg gaactgcatt tccgcatcta ccgcttcgac acgccgttgc agccgggcca
      421 gcgcacggag atccgcttca aaaccgtgcg cgagcagatc ggcttccgca acaacaacaa
      481 cgaaagccgg atcgtcgaga acggcacctt cttgaccagc cacgagatca caccggtgct
      541 cggcgtgcac aacctgttcg cgctgcagga gcgcaccgcg cgccgccact acggcctgcc
      601 tgacgaactg cgcccgccga agctcgacga caatggtccg cgcggcaaca attatttccg
      661 tccggacagt gactgggtca atgccgacct gaccatccat gtcgcggcgg accagacggt
      721 cgtcgccccc ggggagcgtg tcgaagacac ggttgccgga agtcgccgca cgacacactt
      781 caggaccacg gcgcccatcc tgcacttctt caccatccag tcggcgcgct acgccgagcg
      841 caaggagcaa tggaaggacg tcgcgctgga ggtctactac cacccgggcc acccgtacaa
      901 cattgaccgc atgctgcgcg ccatgcgcgc ggcgctggaa tactactcga cgaacttcag
      961 cccctaccag ttcaagagcc tgcgcatcgc cgaattcccg gcgtaccgcg acttcgccca
     1021 ggcgtacccc ggcacgatcg cctacggcga ggccagcggc ttcaccaacg gcacgccgcg
     1081 caattacgac ggcgtcacgc gcgtgaccgc gcacgagacc gcgcaccaat ggtgggcgca
     1141 ccaggtgatc ggcgccgaca tgcagggcca gaccgtgctc tccgagacac tggcggagta
     1201 ctccgcactg atggtgatgg agaagatcaa cgggccggat gagatcaacc gcttcctgcg
     1261 cgcatcgctc gattcctacc tgcgggcacg cggctcggac atcaccggcg aagtgccgct
     1321 ggaactggtc gaggagcagc cgcacatccg ctataccaag ggcggcatgg tgatgtacct
     1381 gctcaaggac ctgatgggcg aggacgcggt caaccgcgcg ctgcgctcgc tgatccagga
     1441 ctatgccttc aagggaccgc cttacccgac cgcgcacgac ctgactcgcc gcctgcgcgc
     1501 gcaggcgccg cccgaactgc ttccgctgat cgccgacctg ttcgagcaga tcaccaccta
     1561 cgacttctcg gtggtgaatg cgcgcagcgc agtgcggccc gatggcaaat gggacgtgac
     1621 gatcgagctc gatgcacaca agtactacgc cgacggcaag ggtgcgcagc gggaggcgcc
     1681 gctcgacacg ctgatcgacg tcggcgtgtt taccgccgag ccaggcaagc cgggcttcgg
     1741 cgccggcagc gtgctgcacc tggcgcacca cgcggtcagg agcggccgcc agacgttgac
     1801 gctggtcgtg gaccgggagc cgaagtttgt cggcgccgac ccttacagca agtacatcga
     1861 catcgtgccc aacgacaaca tcctggccct cgacggccgg cagtaaccgc atcctcagat
     1921 ctgcccatga ccaacccgtt tctggcccgc gccgcctcct cgatcttccg ctacggcggc
     1981 agctttgccg acttcgtgcc ggttcgcgcc gagggttcgt tcctgtacga cagcgccggc
     2041 cgccgcgtgc tggatttcac ctccggccag atgagcgcga tcctcggcca cagtcacccc
     2101 gagatcaccg cgacggtgtg cgagtgggcc ggcaagctgg cgcacctgca cagcagcatg
     2161 ctgtccgagc cggtggtcgg gctggccgaa gcgctggtga agctggcacc cgggcttgac
     2221 cgcgtattgc tgttgacgac cggcgccgag tcgaacgagg ccgcgctgaa gatggcgcgc
     2281 ctggccacgc gcggctatga agtggtcggt ttcggccagt cctggcacgg catgaccggc
     2341 ggcgccgcgg cggcgacgta ttcggcggga cgcaagggct acgggccgat gggtgtcggc
     2401 aatctcgcca tcatggcgcc caatccatac cgcccgacat tcaccgccaa cgggaattac
     2461 gattggcgcg ccgagctcga ttacggcttc gagctggtcg atcgacagtc gaccggatcg
     2521 ctggcggcgt tcatcgccga gcccattctc agttccggcg gcatactcga attgccggag
     2581 ggctatctgg ccgcgctgaa ggagaaatgc cgcgagcgcg gcatgatgct ggtgctcgac
     2641 gaggcgcaga ccggcatcgg ccgcaccggc aataccttcg cgttcgagcg tgatggcgtg
     2701 gtgccagaca tccttacgct gtcgaaaacc attggcgccg ggctgccgct cgcggccgtg
     2761 atggcttcgg cggcgacgga ggcgctgtgt cacgagcgcg gcttcctgtt ttacaccacc
     2821 cacgtgtccg atccgctacc tgccgcgatc ggtctcaccg tgctgaaagt tcttgcccgc
     2881 gagaatctga ccgcgcgcgc gcgcgcagcc ggcgcacggc tcaaggctgg actgcaggcg
     2941 atgatggccg agtttgactg cgtgggcgac gtccgcggcc gcggcctgct cctggggatg
     3001 gaaatcgtca aggacaagca gtcgaaggcg gccgcgcccg agctgggctc gcgcatcacc
     3061 gagcacgcca tgcgcttcg
//
LOCUS       DASZZC010000461         6322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_293338,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000461 DASZZC010000000
VERSION     DASZZC010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293338"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VHE37_09770"
     CDS             complement(<1..223)
                     /locus_tag="VHE37_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35862.1"
                     /translation="MKIPVLIALALGMLLNACASVPPGEDARGAALRFSATPVLDAIA
                     RFQHDRGRPPSSMYELVPDYLKSLPPEPAV"
     gene            484..1764
                     /locus_tag="VHE37_09775"
     CDS             484..1764
                     /locus_tag="VHE37_09775"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVT35863.1"
                     /translation="MASIRKVAILGGARIPFARSNTAYTNKSNQDMLTAAFKGVVDKF
                     QLHGERLGEAAAGAVLKHSRDFNLCRESVMSSGLALETPGCDLQQACGTGLEAAIYIG
                     NKIALGQIESGLAGGSDTTSDAPIAVGEKLRKILLEVNRAKTGGQRLKALSKFRPSML
                     VPAIPTNGEPRTRMSMGQHCELMAQEWHIPREEQDQLAFESHNKAARAYESGFFADLL
                     VPYAGLERDNNLRPDISLEKLASLGPAFDKKSGKGTLSAGNSTPLTDGASAVLLSSED
                     WARAHGLKIQAYIAHAEVAAVDFTNKKEGLLMAPCYAVPRMLARARLTLQDFDFYEIH
                     EAFAAQVLCTLRAWEDRKFCTERLGLPGPLGSIDRSKLNIRGSSLAVGHPFAATGGRI
                     LATAAKILDENGGGRCLVSICAAGGMGVTAIVEK"
     gene            1770..2003
                     /locus_tag="VHE37_09780"
     CDS             1770..2003
                     /locus_tag="VHE37_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35864.1"
                     /translation="MAGKLSKSQQLVMNWLSKGWKAYAVPGGRVEVNGMKVGTLATME
                     SLEKLGLIARLGVSAWEATDAGRALQPPPRESS"
     gene            2104..2514
                     /locus_tag="VHE37_09785"
     CDS             2104..2514
                     /locus_tag="VHE37_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVT35865.1"
                     /translation="MRFMMLMIPRGYESAAPGTMPDAKAVEAMMKYNQQLQDAGVLLV
                     LDGLHPPSMGARVSFKSGKAQVTDGPFAETKEVLGGYWMIKVKSRAEAIEWASRCPAG
                     DNEIIEVRQVQEMEDFSDDVKQAAAGFDQLQKSR"
     gene            complement(2511..3110)
                     /gene="metW"
                     /locus_tag="VHE37_09790"
     CDS             complement(2511..3110)
                     /gene="metW"
                     /locus_tag="VHE37_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HVT35866.1"
                     /translation="MRPDLELIGDWIQPGARVLDLGCGDGALLRHLAATRRVRGYGLE
                     IDAENLARCVDAGVNVIQADIDDGLKDFEAQSFDCVLMAQALQALQRPDHAVAEMLRV
                     GKTAVVTFPNFGNWRVRAALALGRMPVTAALPDRWYDTPNIHLCTVADFEDLCRARGW
                     RIADRRLLNRELRQGPSIVLAPNLFCEVAMYLLEGTRAA"
     gene            complement(3113..4252)
                     /locus_tag="VHE37_09795"
     CDS             complement(3113..4252)
                     /locus_tag="VHE37_09795"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HVT35867.1"
                     /translation="MAAGEGGVGRVQPQKIVIGQPLLLDCGRTLPRHELMVETYGTLN
                     AAKSNAILICHALSGDHHAAGWYDANDRKLGWWDNVIGPGRPLDTERFFVVCLNNLGG
                     CKGSTGPNTANPDTGAPWGPDFPLLTVRDWVRSQALLADQLGIAQWNAAIGGSLGGMQ
                     VMQWAIDLPQRIRHGIVIAAAPRISAQNIAFNEIARQAILSDPDFHGGRFYDKGVVPS
                     RGLKLARMLGHITYLSDEAMRAKFGRELKANQMSFNYDVEFEVESYLRYQGQSFVDRF
                     DANTYLIMTKALDYFDPAREFGGDLVAAFRRAQAKFLVMAFSSDWRFSPARSREIVKA
                     LVDAGRDVSYALIESQLGHDDFLMPIPQYHRVLGSYLRRTLEGSA"
     gene            complement(4252..4809)
                     /locus_tag="VHE37_09800"
     CDS             complement(4252..4809)
                     /locus_tag="VHE37_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HVT35868.1"
                     /translation="MAANFSNAFNHLLDTLLNLYMAALILRILLQWLRAEFHNPFSQF
                     IWRVTNPPVLTLARFVPKWRRLDLAACIVLLLLSLLTIYAGAWMWRRPLELGDALWWA
                     LLKLLWVAINLYTLTLVVQWLLVWFGPGISSPAGSVLYVINEPLLRPVRRRIPPVSGF
                     DLSPLIVMLALQFVMQLLGLPSWFQ"
     gene            complement(4809..5624)
                     /gene="proC"
                     /locus_tag="VHE37_09805"
     CDS             complement(4809..5624)
                     /gene="proC"
                     /locus_tag="VHE37_09805"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HVT35869.1"
                     /translation="MQADIAFIGGGNMATAMIGGLVAGGCARERIRVAEPSAGRAAWL
                     QQEFGVRVAAAGRDVAAGAAAIVLAVKPQQMRAALAGLKPDDGTTIVSIAAGVRLATL
                     QKSLGVRVHYVRTMPNTPALVRKGVTGMYAPAGTPEAARKLAERILQTTGATVWLERE
                     EQMDAVTALSGSGPAYFFLLTEVLREAGEKLGLAPEVAAQLAQQTCIGSAAMINADSD
                     VRTLRAQVTSKGGTTEAAVASLEAAGIRDMFARALAAAARRGGELGDQLERDG"
     gene            5696..>6322
                     /locus_tag="VHE37_09810"
     CDS             5696..>6322
                     /locus_tag="VHE37_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVT35870.1"
                     /translation="MNAPARPPAPAAETQKILDHYRARIEAARVYDVACISSLDEASR
                     LSQRLGNRVLLKREDEQAVHSFKLRGAYNRIAQLSDEERARGVVTASAGNHAQGVALA
                     AKKFGLTAWIVMPRTTPGVKVDAVRALGGKAILHGDAYDDAREHAYALAAEKGMTMVH
                     PYDDPDVIAGQGTIGRELLQQCAELDTALDYVFVPVGGGGLLAGVAAYI"
BASE COUNT         1108 a         2133 c         2150 g          931 t
ORIGIN      
        1 acaccgccgg ctccggcggc aggctcttca ggtaatcggg caccagttcg tacatcgagc
       61 ttggcgggcg gccgcggtca tgctggaagc gggcgatggc gtcgagcacc ggcgtggcgg
      121 aaaaacgcag cgcggcgccg cgcgcgtctt cgcccggcgg gacgctggca caggcgttca
      181 gcagcatgcc cagagcaagc gcgatcaaga ccggaatttt catgcgcgcg agattagcac
      241 cggctgccgg cagcggctgc tgcacagaaa aatgctccac cccaccggct gcaggcatgt
      301 tatttaatat cgtatgattg aataattata cattttttaa taacacgctg aagatcgatg
      361 cggtgcaggc aaaagcgccc gcgcacttgg cctcaccgca ggcggggctg gccgcagcgc
      421 gaatcaatcg gcaaaaaccc tcggctacaa tttcacccga cactttcccc tcggagtttt
      481 tccatggcca gcatccgcaa ggtcgccatc ctcggcggcg cgcgcattcc cttcgcccgc
      541 tccaacacgg cgtacaccaa caagagcaac caggacatgc tgaccgccgc gttcaagggc
      601 gtggtcgaca agttccagct gcacggcgag cgcctgggcg aagcggcggc cggcgcggtg
      661 ctcaagcact cgcgcgactt caacctgtgc cgcgagtcgg tgatgagctc gggcctggcg
      721 ctggaaactc cgggctgcga cctgcagcag gcctgcggca ccgggctgga agcggccatc
      781 tacatcggca acaagatcgc gctcggccag atcgagagcg gcctggccgg cggcagcgac
      841 accaccagcg acgcgccgat cgcggtcggc gagaagctgc gcaagatcct gctcgaggtg
      901 aaccgcgcca agaccggcgg ccagcggctg aaggcgctga gcaaattccg cccgtcgatg
      961 ctggtgccgg ccattcccac caacggcgag ccgcgcacgc gcatgagcat gggccagcac
     1021 tgcgagctga tggcgcagga gtggcacatc ccgcgcgagg agcaggacca gctggcattc
     1081 gagtcacaca acaaggccgc gcgcgcctac gaatccggct tcttcgccga cctgctggtg
     1141 ccctacgccg ggctggagcg cgacaacaac ctgcgcccgg acatctcgct ggagaagctg
     1201 gcgtcgctcg gcccggcctt cgacaagaag agcggcaagg gcacgctgag cgcgggcaac
     1261 tccacgccgc tcaccgatgg cgcctccgcc gtgctcctgt ccagcgagga ctgggcgcgc
     1321 gcgcacgggc tgaaaatcca ggcctacatc gcgcacgccg aagtcgccgc ggtagatttc
     1381 accaacaaga aggaaggcct gctgatggcg ccgtgctacg cggtgccgcg catgctggcg
     1441 cgcgcgcgcc tgacgctgca ggacttcgac ttctacgaga tccacgaagc cttcgccgcg
     1501 caggtgctgt gcacgctgcg cgcgtgggaa gaccgcaagt tctgcacaga gcgcctcggc
     1561 ctgcccggcc cgctgggcag catcgaccgc agcaagctca acatccgcgg ctcgtcgctc
     1621 gccgtcggcc acccattcgc cgccaccggc ggacgcatcc tggccaccgc ggccaagatc
     1681 ctcgacgaga acggcggcgg ccgctgcctg gtgtcgatct gcgccgccgg cggcatgggc
     1741 gtgacggcga tcgtcgagaa gtagcgcgca tggccggcaa gctcagcaag tcgcagcaac
     1801 tggtgatgaa ctggctgagc aagggctgga aggcctacgc cgtgcccggc gggcgcgtcg
     1861 aggtcaacgg catgaaggtc ggcacgctgg cgacgatgga gtcgctggag aagctgggac
     1921 tgatcgcgcg cctgggcgtg tccgcgtggg aagccaccga cgccgggcgc gcgctgcagc
     1981 cgccgccgcg cgagtcctcc tgaaaaaatt tttatgtgcg gctgtcgaag cgccggcgcg
     2041 ccgttcgtct agctgacgaa ggccgccgct tccggccggc cgccgaaaca caggagagtt
     2101 gccatgcgct tcatgatgct gatgattccc cgcggctacg aaagcgccgc gcccggcacc
     2161 atgcccgacg ccaaggcggt cgaggcgatg atgaagtaca accagcagct ccaggacgcc
     2221 ggcgtgctgc tggtgctcga cggcctgcat ccgccgtcga tgggcgcgcg cgtcagcttc
     2281 aagagcggca aggcgcaagt gaccgacggc ccgttcgccg aaaccaagga agtgctgggc
     2341 ggctactgga tgatcaaggt gaaatcgcgc gccgaggcca ttgagtgggc cagccgctgc
     2401 ccggccggcg acaacgagat catcgaagtg cgccaggtgc aggagatgga agatttttcc
     2461 gacgacgtga agcaggccgc cgcaggtttc gaccagttgc agaaatcgcg ctaggcggcg
     2521 cgtgttcctt ccagcaggta catcgccacc tcgcagaaca ggttgggcgc cagcacgatg
     2581 ctcggcccct gcctcagttc ccggttgagc aggcggcggt cggcgatgcg ccagccgcgc
     2641 gcgcggcaca ggtcctcgaa gtcggccacg gtgcacaggt ggatgttggg cgtgtcgtac
     2701 cagcggtcgg gcaacgcggc ggtcaccggc atgcggccca gcgccagcgc cgcgcgcacg
     2761 cgccagttgc cgaagttggg aaaggtgacc accgcggtct tgcccacgcg cagcatctcg
     2821 gccaccgcgt ggtcggggcg ctgcagcgcc tgcagcgcct gcgccatcag cacgcagtcg
     2881 aacgactgcg cctcgaaatc cttgaggccg tcgtcgatgt cggcctggat cacgttgacg
     2941 ccggcatcga cgcagcgcgc gagattttcc gcgtcgattt ccaggccgta gccgcgcacc
     3001 cgccgcgtgg ccgccaggtg cctgagcagc gcgccgtcgc cgcaacccag gtcaagcaca
     3061 cgcgcgccgg gctggatcca gtcgccgatc agttcgaggt cggggcgcag gctcatgccg
     3121 aaccctccag cgtgcggcgc aggtagctgc cgagcacgcg gtgatactgc gggatcggca
     3181 tgaggaaatc gtcgtggccc agctgcgatt cgatcagcgc gtaggagacg tcgcggccgg
     3241 catcgaccag cgccttgacg atctcgcgcg agcgcgcggg cgagaagcgc cagtccgacg
     3301 agaacgccat caccaggaat ttcgcctgcg cgcgccggaa cgcggcgacc aggtcgccgc
     3361 cgaactcgcg tgccgggtcg aagtagtcca gcgccttggt catgatcagg taggtgttgg
     3421 catcgaagcg gtcgacgaag ctctgcccct ggtagcgcag gtagctctcc acctcgaact
     3481 cgacgtcgta gttgaaggac atctggttgg ccttcagctc gcggccgaac ttggcgcgca
     3541 tcgcctcgtc cgagagatag gtgatgtggc cgagcatgcg cgccagcttg agcccgcgcg
     3601 acggcaccac gcccttgtcg tagaaacggc cgccgtggaa atccggatcg gacaggatgg
     3661 cctggcgcgc gatctcgttg aaggcgatgt tctgcgcgct gatgcgcggc gcggcggcga
     3721 tgacgatgcc gtggcggatg cgctgcggca ggtcgatggc ccactgcatc acctgcatgc
     3781 cgccgagcga accgccgatg gcggcgttcc attgcgcgat gcccagctgg tcggccagca
     3841 acgcctgcga gcgcacccag tcgcgcacgg tgagcagcgg gaagtccggc ccccacggcg
     3901 caccggtgtc gggattggcg gtgttgggcc cggtggagcc cttgcagccg ccgagattgt
     3961 tcaggcagac gacgaaaaat ctttccgtgt cgagcgggcg gccggggccg atgacgttgt
     4021 cccaccagcc cagcttgcgg tcgttggcgt cgtaccagcc ggcggcgtgg tgatcgcccg
     4081 acagcgcgtg acagatgagg atggcgttgg acttcgcagc gttgagcgtg ccgtaagtct
     4141 ccaccatcaa ctcgtgccgc ggcagcgtgc gcccgcagtc gagcagcagc ggctgcccga
     4201 tgacgatctt ctgcggctgt acgcggccga cgccgccctc gccggcggcc atcactggaa
     4261 ccacgacggc aggccgagca gctgcatgac gaactgcagc gccagcatga cgatcagcgg
     4321 cgacaggtcg aaacccgaca ccggcggtat gcgccggcgc accgggcgca acagcggctc
     4381 gttgatcacg tagagcacgc tgccggccgg gctgctgatg ccggggccga accagaccag
     4441 cagccactgc accaccagcg tgagcgtgta caggttgatt gcaacccaca gcagcttgag
     4501 cagcgcccac cacagcgcgt cgccgagctc cagcggccgc cgccacatcc acgcgccggc
     4561 gtagatggtc agcagcgaca gcagcagcag cacgatgcag gcagcgaggt cgagccggcg
     4621 ccatttcggc acgaagcgcg ccagagtcag caccggcgga ttggtcacgc gccagatgaa
     4681 ctgcgagaac gggttatgga attccgcgcg cagccactgc agcaggatgc gcaggatcag
     4741 cgccgccatg tacaggttga gcagcgtgtc gagcagatgg ttgaaggcgt tgctgaagtt
     4801 ggcggccatc agccgtctcg ctccagctgg tcaccgagtt cgccgccgcg gcgcgcggcc
     4861 gcggccagcg cccgcgcgaa catgtcgcgg atgccggcgg cctcgagcga agcgacggct
     4921 gcctcggtgg tgccaccctt ggacgtcacc tgcgcgcgca gcgtgcgcac gtcggaatcg
     4981 gcgttgatca tcgccgcaga gccaatacag gtctgctgcg ccagctgcgc ggccacttcc
     5041 ggcgccagcc ccagcttctc gccggcctcg cgcaacacct cggtgagcag gaagaaatac
     5101 gcgggcccgg agccggacag cgcggtcacc gcgtccatct gctcttcgcg ttccagccac
     5161 accgtggcgc cggtggtctg caggatgcgt tccgccagct tgcgtgcggc ttccggcgta
     5221 cccgcgggcg cgtacatgcc ggtcacgccc ttgcgcacca gcgcgggcgt gttgggcatg
     5281 gtgcgcacgt agtgcacgcg cacgcccagc gatttctgca gtgtggccag ccgcacgccg
     5341 gcagcgatgg acacgatggt ggtgccgtca tccggcttga gtccggccag cgccgcgcgc
     5401 atctgctgcg gcttgaccgc gagcacgatg gccgctgcgc cggctgcgac gtcgcggccg
     5461 gcagcagcga cgcgcacgcc gaattcctgc tgcagccagg ccgcgcgccc ggcgctgggc
     5521 tcggccacgc ggatacgttc gcgcgcacag ccgccggcga caagcccgcc gatcatggcc
     5581 gtcgccatgt tgccgccgcc gatgaatgcg atgtctgcct gcacgctaga aatccagagc
     5641 caaaatcgaa gcgcgctatg atattgcacg ctgccttcca actctaattt tcctcttgaa
     5701 cgctcccgcc cggcccccgg cccctgccgc ggaaacgcag aagatcctcg accactaccg
     5761 agcgcgcatc gaagccgcgc gcgtgtatga cgtggcctgc atttccagcc tggacgaggc
     5821 atccaggctg tcccaacgcc tgggcaatcg tgtgctgctc aagcgcgagg atgagcaggc
     5881 ggtgcattcg ttcaagctgc gcggcgccta caaccgcatc gcgcaactgt cggacgagga
     5941 gcgcgcgcgt ggcgtggtca ccgcctcggc gggcaaccac gcacagggcg tggcgctggc
     6001 agcgaagaaa ttcggcctga ccgcgtggat cgtgatgccg cgcaccacgc cgggcgtgaa
     6061 ggtggacgcg gtgcgcgcgc tcggcggcaa ggccatcctg cacggcgatg cctacgacga
     6121 tgcgcgcgag cacgcctatg cgctggcggc ggaaaaaggc atgaccatgg tgcacccgta
     6181 cgacgatccc gacgtgatcg ccggccaggg caccatcggc agggaactgc tgcagcagtg
     6241 cgcggaactc gacacggcgc tcgactatgt ttttgtcccg gtaggtggcg gcggtttgct
     6301 cgccggtgtt gccgcctata tc
//
LOCUS       DASZZC010000462         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1064163,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000462 DASZZC010000000
VERSION     DASZZC010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064163"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VHE37_09815"
     CDS             complement(<1..627)
                     /locus_tag="VHE37_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HVT35871.1"
                     /translation="MSHDHHHHGHAHHHDHGHAHDHGHDHAQDRAHDHAHDHSQGHAH
                     GHGRSESRLLIALVVSALFMLLEVGAGLYAKSLALIADAGHMLTDAVSLMLAYIAIRV
                     ARRPADAARSYGYERLQVLSAFVNGLSLLVISVWIIAEAAERAWHPVPIDGRTMLWVA
                     LLGVAVNAIAFFILHDGDNENLNMRAAVAHVLSDLAASAATVLAALVIL"
     gene            complement(719..>2996)
                     /locus_tag="VHE37_09820"
     CDS             complement(719..>2996)
                     /locus_tag="VHE37_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35872.1"
                     /translation="FIDNYAHDNVGGFLIFDLPGRVQFGEKNIVHQNKSYHNNIKSFA
                     PRGAIVGDVPSGTGALVLASDQLEFYDNDVQDNDTVGLTIVNYGLVDNNEDQRRYDYF
                     PEGMHIYNNTFTNNGGNPQLPDPNKDTCTGPNGLPGPSDDPSCISNNATLLVTIIQLK
                     NGGHSAQIVWDGGLDSDTGCTDIPVDSYGVPLTQPDPQDTTRYEARHDERGRPNLYQY
                     DPLPKCKYNHWKFNDDGSLKKPANGMCIENNTFVTKFDPQRPGALAVNDFTNIHFSTA
                     DPTAAMNLVPAEATPLSTPPHDCPIADAPLLVQYVPVLGSFTPNPADDPRASDAQISQ
                     VCGGGEPGKINYAALAQFNCPKLSDYGLFADPADPTKNPNGFGVPFNLNTILFSDYAV
                     KSRFLFLPPDASGNVQKATYEDHQQCDTINIYDCYTATLGFPVGTVFAKTFAFRNQGS
                     PDNVVETRLLIKRQHADGSVYWVAFAYEWQTVNGVRQALLKLEGDTQGVSWNYDDEDP
                     NAKDASGTRLHYQGSTPNYGVPNAGACLLCHGGDDREPGAAPIGPKVRNLNKDYTYAD
                     GTTANQLTYLAARGLLDLPAGKTADQLEKLPKFNVIGSGDASDTANDEHLRVRAFLEV
                     NCMHCHNPAGAAQNSGLSLDSFTEPMDEGHGICKPPIAAGKAADNVNYDIVPGSSGGS
                     LLAVRVNSTQPGIKMPPLARTVVDTEAANLITHWIDNVVSAHADPNANKCGTGSSTLP
                     VPLMKVIPATPAQKAPFG"
BASE COUNT          450 a          895 c         1068 g          583 t
ORIGIN      
        1 gaggatgacc agcgccgcga gcacggtggc ggccgacgcc gcgaggtcgg acagcacgtg
       61 cgccaccgcg gcgcgcatgt tgagattctc gttgtcaccg tcatgcagga tgaagaaggc
      121 gatggcattg accgcgacgc cgagcaacgc cacccacagc atggtgcggc catcgatcgg
      181 caccgggtgc caggcgcgct cggccgcttc cgcgatgatc cacaccgaga tcaccagcag
      241 cgacaagccg ttgacgaagg ccgacagcac ctgcaggcgc tcgtagccgt aggaacgcgc
      301 cgcgtccgcc ggccggcgcg cgacgcggat ggcgatatag gccagcatca gcgataccgc
      361 gtcggtcagc atgtggccgg cgtcggcgat cagcgccagc gatttcgcgt agaggcccgc
      421 accgacttcc agcaacatga acagcgcgct caccaccagt gcgatcagta ggcgcgactc
      481 gctgcggccg tgcccatgcg catgaccctg tgaatggtcg tgtgcatgat catgcgcgcg
      541 atcctgcgca tggtcgtggc cgtgatcgtg cgcatgaccg tgatcatgat gatgcgcgtg
      601 gccgtgatga tggtggtcgt ggctcatgcg cgcattgtag gtgcaaggcc atggcaaagc
      661 ggtttctgcc gcgatcctga aaggtaaaga ttgcggcaga aacgtgtggc gttttgcgct
      721 agccgaacgg cgccttctgt gccggcgtgg ccgggatcac cttcatcagc ggaacgggca
      781 gcgtgctgct gccggttccg cacttgttgg cattgggatc ggcgtgcgcg gaaaccacgt
      841 tgtcgatcca gtgcgtgatc aggttggccg cttcggtgtc gaccacggtg cgcgccagcg
      901 gcggcatctt gatgccgggc tgggtggagt tgacgcgcac cgccagcagc gagccgccgg
      961 aactgcccgg cacgatgtcg tagttgacgt tgtccgccgc cttgcccgcc gcgatgggcg
     1021 gcttgcagat gccgtggccc tcgtccatcg gctcggtgaa cgagtcgagc gacaggcccg
     1081 agttctgcgc tgcgcctgcc gggttgtggc agtgcatgca gttcacttcg aggaaggcgc
     1141 gcacgcgcag gtgctcgtcg ttcgccgtat cgctggcatc gccgctgccg atgacgttga
     1201 acttgggcag cttctccagc tggtcggcgg tcttgccggc cggcaggtcg agcaggccgc
     1261 gcgccgccag gtaggtgagc tggttggcgg tggtgccgtc agcgtaggtg tagtccttgt
     1321 tcaggttgcg caccttggga ccgatgggcg ccgcaccggg ctcgcggtcg tcgccgccat
     1381 ggcacagcag gcaggcgccg gcgttgggca cgccgtagtt gggcgtgctg ccctggtagt
     1441 gcaggcgcgt gccgctggca tccttggcat tcgggtcttc gtcgtcgtag ttccacgaca
     1501 cgccctgcgt atcgccttcg agcttgagca gcgcctggcg cacgccgttc accgtttgcc
     1561 attcatacgc gaacgccacc cagtagaccg agccgtcggc atgctggcgc ttgatcagca
     1621 ggcgcgtctc caccacattg tcgggcgaac cctggttgcg gaaggcgaag gtcttggcga
     1681 acaccgtgcc caccgggaag cccagcgtgg cggtgtagca gtcatagatg ttgatggtgt
     1741 cgcactgctg gtggtcttcg taggtggcct tctgcacgtt gccgctggca tccggcggca
     1801 ggaacaggaa gcgcgacttc accgcgtagt cggagaacag gatggtgttg aggttgaacg
     1861 gcacgccgaa gccgttcgga ttcttcgtgg gatcggccgg atcggcgaac aggccgtagt
     1921 ccgacagctt ggggcagttg aactgcgcca gcgcggcgta gttgatcttg cccggctcgc
     1981 cgccgccgca gacctgcgaa atctgcgcat cggacgcgcg cggatcgtcc gccgggttgg
     2041 gcgtgaagct gcccagcacc ggcacgtact gcaccagcag cggcgcgtcg gcaatcgggc
     2101 agtcgtgcgg cggcgtcgac agcggcgtgg cctcggccgg caccaggttc atcgcggccg
     2161 tgggatcggc ggtggagaaa tggatgttgg tgaagtcgtt caccgccagc gcaccgggcc
     2221 gctgcgggtc gaacttggtg acgaaggtgt tgttctcgat gcacatgccg ttggccggct
     2281 tcttcagcga accgtcgtcg ttgaacttcc agtggttgta cttgcacttg ggcagcgggt
     2341 cgtactggta gagattcggc cggccgcgct cgtcgtggcg cgcctcgtag cgcgtggtgt
     2401 cctgcggatc gggctgggtc agcggcacac cgtagctgtc gaccgggatg tcggtgcagc
     2461 cggtatcgct gtcgaggccg ccgtcccaca cgatctgcgc gctgtggccg ccgttcttca
     2521 gctggatgat ggtgaccagc agcgtggcgt tgttgctgat gcagctgggg tcgtcgctcg
     2581 gcccgggcag gccgttgggg ccggtgcagg tgtccttgtt cggatcgggc agctgcggat
     2641 tgccgccgtt gttggtgaag gtgttgttgt agatgtgcat gccttcgggg aagtagtcgt
     2701 agcggcgctg gtcctcgttg ttgtcgacca ggccgtagtt gacgatggtc aggcccacgg
     2761 tgtcgttgtc ctgcacgtcg ttgtcgtaga actccagctg gtcgctggcc agcaccagcg
     2821 cgccggtgcc ggacggcacg tcgccgacga tggcgccgcg cggggcaaaa ctcttgatgt
     2881 tgttgtggta gctcttgttc tggtgaacga tgtttttctc gccgaactgc acgcggcccg
     2941 gcagatcgaa gatcaaaaat ccgccgacgt tgtcgtgcgc gtagttgtcg atgaac
//
LOCUS       DASZZC010000463         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1004683,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000463 DASZZC010000000
VERSION     DASZZC010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004683"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VHE37_09825"
     CDS             <1..260
                     /locus_tag="VHE37_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVT35873.1"
                     /translation="RRKLKTPSGGVLVEEVRNGPAAVAGLRPGDVILQIAGQEVDSPD
                     RFSEVAGRLTPGQTVPMLVQRAGAPVFLSLDVPALATKPQP"
     gene            268..492
                     /locus_tag="VHE37_09830"
     CDS             268..492
                     /locus_tag="VHE37_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HVT35874.1"
                     /translation="MKLTLVGRPGCHLCEEFEHALRAAYPQVEIEHGDVDSRADWLRR
                     YGMKIPVLLAPDGLVLCETFFDPAALAAIR"
     gene            610..2406
                     /gene="lepA"
                     /locus_tag="VHE37_09835"
     CDS             610..2406
                     /gene="lepA"
                     /locus_tag="VHE37_09835"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HVT35875.1"
                     /translation="MSHIRNFCIIAHIDHGKSTLADRFIQLCGGLEAREMTEQVLDNN
                     PIERERGITIKAQAISLNYQAKNGESYQFNIIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQSVANCYTALELHLEVLPILNKIDLQNAEPDRVAEEIEQVIGIEATDALR
                     ISAKTGLNVPDVLEAVVHKLPPPKGDENGKLQALIVDSWFDNYLGVVSLIRVVNGRVR
                     KGQKIRVLSTGKDYEVTMLGVHTPKRVFKDEITTGEVGFLIAGIKDIFGAPVGDTLTD
                     GGQSGVEPLPGFKRVQPRVFAGMFPVDADEFEDFRESLQKLRLNDSALVFEPENSTAL
                     GFGFRIGFLGMLHMEIVQERLEREYNLNLITTAPSVVYEVVMNTGEVKKIDNPAELPD
                     AGQYDELREPIILARVLMPPDYVGPVMQLCMEKRGVQKNLRYLGGQVQLEVEMPLAEV
                     VLDFFDKLKSVSRGYASFEYEFVRFEAADLVKLDVLVNGDKVDALATIVHRSVSQRRG
                     ADLCDRMKEVIHRQMFDVAIQAAIGSKIISRSTVKALRKDVIAKCYGGDVSRKRKLLE
                     KQKEGKKRMKQVGAVEIPQEAFLAVLGVEKKK"
     gene            2443..3225
                     /gene="lepB"
                     /locus_tag="VHE37_09840"
     CDS             2443..3225
                     /gene="lepB"
                     /locus_tag="VHE37_09840"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVT35876.1"
                     /translation="MSDVHFDFAFILTALTLVTGVVWAADRFWLAKRRGEAKPGMVVD
                     FCCSFFPVIALVLGLRTCLTEPFRIPSGSMIPTLQVGDFILVNKFAFGVRDPVFHYKL
                     LKIGEPQRGDIVVFRWPVDPTKDFIKRIVGVPGDHISYRDKVLYINGEKAAQSDQAVF
                     DVGAYGSILRYREDLGGVAHDIHVYAERPSDDFDVTVGEGEYFAMGDNRDGSDDSRRW
                     GMVPEKNLVGRAYLIWLNLILDWDGIKPNFQMDFSRIGTRLH"
     gene            3263..3646
                     /locus_tag="VHE37_09845"
     CDS             3263..3646
                     /locus_tag="VHE37_09845"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HVT35877.1"
                     /translation="MKGPSKQRGMGWFGLMFMFILIGFFGLVGMKCWPIVFNEAKLER
                     AVKSVAHDPEVAAAEAPEKVRSALQKWWNVEDIEFLLPADVKVRNTGNGRVLAYDYWA
                     QTNLFKNVYVSFHYTHEEPFPKSGF"
     gene            3677..4330
                     /gene="rnc"
                     /locus_tag="VHE37_09850"
     CDS             3677..4330
                     /gene="rnc"
                     /locus_tag="VHE37_09850"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVT35878.1"
                     /translation="MRGLGYQFADAGLLDLALTHRSRGARNNERLEFLGDSLINFLVG
                     EALYRLQPLAGEGALSRLRASLVREESLARIARGLDLGEALWLGESEQKSGGHRRDSI
                     LADALEAVIGAVYLDGGLPAAQQVCERLFGPALADLPDAESLKDAKTRLQEELQAQGR
                     PLPRYEVIEESGPAHRRHFLVRCALADAAQETQASAASRRHAEQRAAELMIQQLEAK"
     gene            4331..5215
                     /gene="era"
                     /locus_tag="VHE37_09855"
     CDS             4331..5215
                     /gene="era"
                     /locus_tag="VHE37_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HVT35879.1"
                     /translation="MRSGMVTILGRPNVGKSSLLNALVGQKVSIVSHKPQTTRHRIQG
                     VLHHADGQIVFVDTPGLHRGGKKALNRAMNEAATGAVSGVDLVLFVIEAGRFTEEDQA
                     VLDQLKDESAPVALVINKVDRVDDKEKLLPELQQRSSLREFAFVVPLSATKKANLDAL
                     VKEILARLPEGDPLYPEDMIAGHDMSFTVSELVREKLTRTLHQELPYALTVQIEEYKQ
                     EGRLDRISAVIWVEREGQKKIVIGEQGAALKAVGSATRRELERMTGRKVFLKLWCKVR
                     ENWSDDPGALKTFGIVQE"
     gene            5212..5907
                     /gene="recO"
                     /locus_tag="VHE37_09860"
     CDS             5212..5907
                     /gene="recO"
                     /locus_tag="VHE37_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HVT35880.1"
                     /translation="MTRQRISLEPGYVLNARAYTDSSLLVEAWTRAHGRVGLVARGAR
                     AARSKLRGALQPLLPLLLSWTESGELGTLTGAEADGAPWSLSGERVFYGWYVNELLLK
                     LLQRRDPHPQLYPVYAQTLALLAGEHAEAALRVFELALLTESGYGLPLPDEIDAAAQY
                     RFDVQHGATIVGREAGSVSGASLIALRDQHFDTPQALADARKILRAAIALQLNGRDLE
                     TPKLLRELRAYTE"
     gene            5940..>6099
                     /locus_tag="VHE37_09865"
     CDS             5940..>6099
                     /locus_tag="VHE37_09865"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HVT35881.1"
                     /translation="MPRLRLGVNVDHVATIRQARGTSYPDPVEAALAAVRAGADSITV
                     HLREDRRHI"
BASE COUNT         1081 a         1949 c         2090 g          979 t
ORIGIN      
        1 accgcaggaa gctgaagacg ccctcgggcg gcgtgctggt cgaggaagtg cgcaacggcc
       61 cggcggccgt cgccggactg aggccgggcg acgtgatcct gcagatcgcc ggccaggaag
      121 tggacagccc cgaccgcttc agcgaagtgg ccggccggct cacgcccggc cagaccgtgc
      181 cgatgctggt gcaacgcgcc ggtgcgccgg tgttcctctc gctcgacgtg cccgcgctcg
      241 ccaccaagcc gcaaccctga cgcggacatg aaactcaccc tggtcggcag gcccggctgc
      301 cacctgtgcg aggagttcga gcacgcgctg cgcgcggcgt atccgcaggt ggagatcgag
      361 cacggcgacg tcgacagccg cgccgactgg ctgcgccgct acgggatgaa aatcccggtg
      421 ctgctggcgc cggatggcct ggtgctgtgc gagaccttct tcgatcccgc cgcgctggcc
      481 gcgatcaggt gaggtgatcg cctggggaac aaccggccgc gcgtccggtc acaggcggcg
      541 tctggccgcg gcggagcagg tatacttcgc ggcctttttt gccgccggaa ttccgttgcc
      601 gccgtgaagc tgtcgcatat aagaaacttc tgcatcatcg cgcacatcga tcacggaaaa
      661 tcgaccctgg ccgaccgctt catccagctt tgcggcgggc tcgaggcgcg cgagatgacc
      721 gagcaggtgc tcgacaacaa cccgatcgag cgcgagcgcg gcatcaccat caaggcgcag
      781 gccatttcgc tgaactacca ggcgaagaac ggcgagagct accagttcaa catcatcgac
      841 acgcccggcc acgtggactt ctcctacgaa gtctcgcgtt cgctgtcggc ctgcgaaggc
      901 gcgctgctgg tggtcgatgc ctcgcagggc gtcgaggcgc agagcgtggc caactgctac
      961 accgcgctgg aactgcacct ggaagtgctg ccgatcctga acaagatcga tctgcagaac
     1021 gccgagccgg accgcgtggc cgaggaaatc gagcaggtca tcggcatcga ggccaccgac
     1081 gcgctgcgca tctcggccaa gaccggcctc aacgtgcccg acgtgctgga agcggtggtg
     1141 cacaagctgc cgccgccgaa aggcgacgag aacggcaagc tgcaggcgct gatcgtcgat
     1201 tcctggttcg acaactacct gggtgtggtc tcgctgatcc gcgtggtcaa cggccgcgtg
     1261 cgcaaggggc agaagatccg cgtgctgtcc accggcaagg actacgaagt gaccatgctg
     1321 ggcgtgcata cgcccaagcg cgtgttcaag gacgagatca ccaccggcga agtgggcttt
     1381 ctcatcgccg gcatcaagga catcttcggc gcgccggtgg gcgatacgct gaccgatggc
     1441 gggcagagcg gggtcgagcc gctgccgggc ttcaagcggg tgcagccgcg cgtgttcgcc
     1501 ggcatgttcc cggtcgacgc cgacgagttc gaggatttcc gtgagagcct gcagaagctg
     1561 cgcttgaacg attcggcgct ggtgttcgag ccggaaaact ccaccgcgct gggcttcggt
     1621 tttcgcatcg gcttcctggg catgctgcac atggagatcg tgcaggagcg cctggagcgc
     1681 gagtacaacc tcaacctcat caccaccgcg ccttcggtgg tctacgaagt ggtgatgaac
     1741 accggcgagg tcaagaagat cgacaacccg gccgagctgc ccgacgccgg ccagtacgac
     1801 gagctgcgcg agccgatcat cctggcgcgc gtgctgatgc cgccggacta cgtcggcccg
     1861 gtgatgcagc tgtgcatgga aaagcgcggt gtgcagaaaa acctgcgcta cctcggcggc
     1921 caggtgcagc tggaagtgga aatgccgctg gccgaggtgg tgctggattt cttcgacaag
     1981 ctgaagagcg tatcgcgcgg ctatgcctcg ttcgaatacg agttcgtgcg cttcgaggcg
     2041 gccgacctgg tcaagctcga cgtgctggtc aacggcgaca aggtcgacgc ccttgcaact
     2101 attgtccacc gcagcgtgtc tcagcggcgc ggcgccgacc tgtgcgaccg catgaaagag
     2161 gtcatccacc ggcagatgtt cgacgtcgcc atccaggcgg cgatcggctc caagatcatc
     2221 tcgcgctcga cggtcaaggc actgcgcaag gacgtgatcg ccaagtgcta cggcggcgac
     2281 gtgtcgcgca agcgcaagct gctggagaag cagaaggaag gcaagaagcg catgaagcag
     2341 gtgggcgcgg tggagattcc gcaggaagcc ttcctggccg tgcttggcgt cgagaagaag
     2401 aagtagcccc ttactcaacc tcatcaccta cactagcgcc ccatgtccga cgtgcatttc
     2461 gactttgcct tcatcctcac cgcgctcacc ctggtcaccg gcgtggtgtg ggcggccgac
     2521 aggttctggc tggccaagcg ccgcggcgag gccaagcccg gcatggtggt cgatttctgc
     2581 tgctcgtttt tcccggtgat cgcgctggtg ctggggctgc gcacctgcct gaccgagccg
     2641 ttccgcatcc cgtccggttc gatgattccg accctgcagg tcggcgactt catcctggtc
     2701 aacaagtttg ccttcggcgt gcgcgacccg gtgttccact acaagctgct caagatcggc
     2761 gaaccgcagc gtggcgacat cgtggtgttc cgctggccgg tggacccgac caaggacttc
     2821 atcaagcgca tcgtcggcgt gccgggcgat cacatcagct accgtgacaa ggtgctgtac
     2881 atcaacggcg agaaggccgc gcagagcgac caggccgtgt tcgacgtcgg cgcctacggc
     2941 tccatcctgc gctaccgcga ggacctgggc ggcgtggccc acgacatcca tgtctacgcc
     3001 gagcggccat ccgacgattt cgacgtgacg gtgggcgagg gtgaatattt cgccatgggc
     3061 gacaaccgcg acggcagcga cgacagccgc cgctggggca tggtgccgga gaagaacctg
     3121 gtcggccggg cctacctgat ctggctcaac ctgatcctcg actgggacgg catcaagccg
     3181 aatttccaga tggatttctc gcgcattggc acccggttac actgagcgca atagcagcgg
     3241 aacctacatt cgggagctcg tcatgaaggg accttcgaag cagcgcggca tggggtggtt
     3301 cggactcatg ttcatgttca tcctgatcgg gtttttcggc ctggtcggca tgaagtgctg
     3361 gcccatcgtg ttcaacgagg cgaagctgga acgcgcggtg aagtcggtgg cgcacgaccc
     3421 cgaagtggcg gccgcggaag ccccggaaaa ggtcaggagc gcgctgcaga aatggtggaa
     3481 cgtcgaggat atcgagttcc tgctgccggc cgacgtcaag gtgcgcaaca ccggcaatgg
     3541 ccgcgtgctg gcctatgact actgggcgca gaccaacctg ttcaagaatg tctacgtgtc
     3601 gttccattac acccacgaag agccgttccc caagtcgggc ttctgaggcg cgcgggcagg
     3661 cccggccggt cacctgatgc gcgggctggg ctaccaattc gccgacgccg gtctgctcga
     3721 tctcgcgctg acccaccgca gccgcggcgc acgcaacaac gagcggctgg agttcctggg
     3781 cgattcgctg atcaatttcc tggtgggaga ggcgctgtac cggctgcagc cgctggctgg
     3841 cgagggcgca ctgtcgcgtc tgcgcgcgag cctggtgcgc gaggaatcgc tggcgcgcat
     3901 cgcgcgcggg ctcgatctcg gcgaggcgct gtggctgggc gagagcgagc agaagagcgg
     3961 tggccatcgg cgcgattcga tcctggccga tgcgctggag gcggtgatcg gcgcggtcta
     4021 ccttgatggc ggattgccgg cggcgcagca ggtgtgcgaa cggctgttcg ggccggcgct
     4081 ggcggatctg ccggatgccg agtcgctgaa ggatgcgaag acgcggctgc aggaggaact
     4141 gcaggcacag gggcggccgt tgccgcgcta cgaagtgatc gaggaatccg gcccggcgca
     4201 ccggcggcat ttcctggtgc gctgcgcatt ggccgacgcc gcgcaggaga cccaggccag
     4261 cgccgccagc cggcgccatg ccgagcagcg cgcggcggaa ctgatgattc aacagctgga
     4321 agcgaaatag atgcgtagcg gaatggtgac catcctcggg cggccgaacg tcggcaagtc
     4381 ctctttgctc aatgccctgg tcgggcagaa ggtgagcatc gtctcgcaca agccgcagac
     4441 cacgcgccac cgcatccagg gcgtgctgca ccatgccgac ggccagatcg tgttcgtcga
     4501 cacgcccggc ctgcaccgtg gcggcaagaa ggcgcttaac cgcgcgatga acgaggccgc
     4561 caccggcgcc gtgtcgggcg tggacctggt gctgttcgtc atcgaggccg gccgcttcac
     4621 cgaggaagac caggcggtgc tggaccagct caaggatgag tccgcgccgg tggcgctggt
     4681 gatcaacaag gtcgatcgcg tcgacgacaa ggaaaagctg ctgcccgagc tgcagcagcg
     4741 ctcgtcgctg cgcgagttcg ccttcgtggt gccgctgtcg gccaccaaga aagcgaatct
     4801 cgatgcgctg gtgaaggaaa tcctggcgcg gctgccggaa ggcgacccgc tgtatcccga
     4861 ggacatgatc gccggccacg acatgagctt caccgtgagc gagctggtgc gcgagaagct
     4921 cacgcgcacg ctgcaccagg aactgcccta tgcgctcacc gtgcagatcg aggagtacaa
     4981 gcaggaaggc cggctggacc gcatcagcgc ggtgatctgg gtggagcgcg aggggcagaa
     5041 gaagatcgtg atcggcgagc agggcgcggc gctcaaggcg gtgggcagcg cgacgcggcg
     5101 cgagctcgag cgcatgaccg ggcgcaaggt attcctcaag ctgtggtgca aggtgcgcga
     5161 gaactggagc gatgatcccg gcgcgctgaa gaccttcggc atcgtgcagg aatgacgcgc
     5221 cagcgcatct cgctggaacc cggctacgtc ctcaacgcgc gcgcctacac tgacagttcc
     5281 ctgctggtgg aggcatggac gcgcgcgcat ggccgcgtcg gcttggtggc gcgcggcgcg
     5341 cgcgcggccc gatcgaaact acgcggcgcg ttgcagccgc tgctgcccct gctgctgtcg
     5401 tggaccgagt cgggtgagct cggcacgctg accggcgccg aggccgacgg cgcgccatgg
     5461 tcgctgtcgg gcgagcgcgt gttctacggc tggtacgtga acgaactgct gctcaagctg
     5521 ctgcagcggc gcgatccgca tccgcagctg tacccggtct acgcgcagac cctggcgctg
     5581 ctcgcgggcg agcatgccga ggccgcgctg cgcgtgttcg agctcgcgct gctcaccgaa
     5641 agcggttacg gcctgccgct gcccgacgag atcgacgccg cagcgcagta ccgtttcgat
     5701 gtacagcacg gcgccaccat cgttgggcgc gaggctggat cggtcagcgg cgccagcctg
     5761 atcgcgctgc gtgaccagca tttcgacacg cctcaggcgc tggccgacgc gcgtaaaatc
     5821 ctgcgcgccg ccatcgcgct gcagctcaac ggccgtgacc tggaaacgcc caagctgctg
     5881 cgcgagctgc gcgcgtacac cgagtaagtt tgccgagtga agtccgcatg acccgcaaga
     5941 ttccgcgcct gcgcctgggc gtcaacgtcg accatgtcgc caccatccgc caggcgcgcg
     6001 gcacctcgta ccccgacccg gtggaagcgg cgctggccgc ggtgcgcgcc ggcgccgaca
     6061 gcatcaccgt gcacctgcgc gaagaccgcc gccacatcc
//
LOCUS       DASZZC010000464         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_616163,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000464 DASZZC010000000
VERSION     DASZZC010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616163"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VHE37_09870"
     CDS             complement(<1..239)
                     /locus_tag="VHE37_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT35882.1"
                     /translation="MEQQHRPQRPQAPMPPQHQQRPGLERDMRPRPQYEGAAYRAAGK
                     LHGKAALITGGDSGIGRAVAVLYAREGADVGIVHLP"
     gene            367..543
                     /locus_tag="VHE37_09875"
     CDS             367..543
                     /locus_tag="VHE37_09875"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HVT35883.1"
                     /translation="MAKYGKAAGKKVKKAMHEKKKGKLKSGSGKKVKSRKQAIAIGLS
                     QARKSGAKVPKKKK"
     gene            556..864
                     /locus_tag="VHE37_09880"
     CDS             556..864
                     /locus_tag="VHE37_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HVT35884.1"
                     /translation="MAPQRIQMRRSAGWRKPAEAVYVGRPTIWGNPWKVGGRAHGAID
                     PATAQRRYEHALLAGGLADRTGHALVDRLEELRGHDLACWCEPSQPCHADVLLRLANR
                     "
     gene            866..1228
                     /locus_tag="VHE37_09885"
     CDS             866..1228
                     /locus_tag="VHE37_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HVT35885.1"
                     /translation="MGDPRVYFAAERTLLAWVRSGIAISGLGFVVSRFGLYLRLLALP
                     APAPLHLESPRLSAVIGAALVGMGAAASLLAALQFRGFIATLPRGDLPQRYHVAFAQL
                     LAYGVAAAGALLCLYLAQ"
     gene            1499..1960
                     /locus_tag="VHE37_09890"
     CDS             1499..1960
                     /locus_tag="VHE37_09890"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HVT35886.1"
                     /translation="MKQSAILFSLALASALSACVAPAPQPPAPGTYVVEQPPPYNSSY
                     CADCGTVTAVTEREVESRPNVGGAIAGAIIGGVVGHQFGSGRGNDVMTAGGAVAGGAV
                     GSQVGRGGGGLVYDLAIRMDDGITRVITVAAPGELRTGSRVRIAGDHVVPI"
     gene            complement(1994..>2402)
                     /locus_tag="VHE37_09895"
     CDS             complement(1994..>2402)
                     /locus_tag="VHE37_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVT35887.1"
                     /translation="SCFCGAVQFTVSGEPAAMGYCHCESCRHWSAGPVNAFSLWQPSA
                     VKVTRGADKVGTYHKTENSYRKWCKDCGGHIFSEHPGMGLTDVYAAVIPSLKFKPALH
                     VNYGEKVLSVKDGLPKMKDFPKEMGGSGVSLPD"
BASE COUNT          371 a          827 c          822 g          382 t
ORIGIN      
        1 ggcaggtgca cgatgcccac gtcggcgcct tcgcgcgcgt agagcaccgc caccgcgcgg
       61 ccgatgccgg agtcgccgcc ggtgatcagc gcggccttgc cgtgcagctt gcccgccgcg
      121 cggtaggccg cgccttcgta ttgcgggcgc ggccgcatgt cgcgttccag tcccggccgc
      181 tgctggtgct gcggcggcat gggcgcctgc gggcgctgtg gtcggtgctg ctgctccatg
      241 tggcgtctcc gtaaaaggcc gatgaaattc gagcgcgctg cggcgcgcgc ggttcgcccg
      301 cgcacctgaa ctttccgctc atgcgtggcg ccagattcag caccggcgcc agaccaggag
      361 agagcgatgg ccaagtacgg caaggcggca ggcaagaaag tgaagaaggc gatgcacgaa
      421 aagaagaaag ggaagctgaa gagcggctcg ggcaagaagg tgaagagccg caagcaggcc
      481 attgccatcg gcctgtcgca ggcgcgcaag tccggcgcca aggtgccgaa gaagaaaaag
      541 tagtgcgtgg cacgcatggc cccgcagcgc atccagatgc ggcgcagcgc cggctggcgc
      601 aagccggccg aggcggtcta cgtcggccgt ccaaccatct ggggcaatcc atggaaggtc
      661 ggcggcaggg cgcacggcgc catcgacccg gccaccgcgc agcgccgcta cgagcacgcg
      721 ctgctcgccg gcgggctcgc cgaccgcacc ggtcacgcgc tggtcgaccg cctggaagaa
      781 ctgcgcgggc atgatctggc ctgctggtgc gagcccagcc agccgtgcca cgccgacgtg
      841 ctcctgcgcc tggccaaccg ctgagatggg cgacccgcgc gtgtacttcg ccgccgagcg
      901 cacgctgctg gcctgggtgc gcagcggcat cgccatcagc ggcctgggct tcgtggtgtc
      961 gcgcttcggc ctctacctgc gcctgctcgc gctgccggcg cctgcgccgc tgcacctgga
     1021 atcgccgcgc ctgtccgcgg tgatcggtgc ggcgctggtg ggcatgggcg ccgccgcgtc
     1081 gctgctggcc gcgctgcagt tccgcggctt catcgctacg ctgccgcgcg gcgatctgcc
     1141 gcagcgctac cacgtggcat tcgcccagct gctggcctac ggcgtggccg cggccggcgc
     1201 gctgctatgc ctgtacctcg cgcagtgact gcgcgcgcgg ctgcaggcac gcgcaggagc
     1261 atgaaccgca ccgcccttca tggtgttatt tattattgta ttaattaata tatagctaat
     1321 aattaataac atagaacgag caggtgcggt aggagcgtcc ggcaatgtaa atcctgccac
     1381 ccagaactcc gcgcgtgccg ccacgtctta ttccaggcag gcgcacgacg acggcgccgc
     1441 gtgaaatttc ctgcgcggat caccgctccg cgccgccgat tcccatcagg agttcgccat
     1501 gaaacagtcc gcgattcttt tcagcctcgc ccttgcgtcc gcgctcagcg cctgcgtggc
     1561 accggcgccg caaccgccgg cgccgggcac ttacgtggtc gagcagccgc cgccgtacaa
     1621 cagcagctac tgcgccgact gtggcaccgt caccgccgtc accgagcgcg aggtggagtc
     1681 gcggccaaac gtgggcggtg ccattgccgg cgccatcatc ggcggcgtgg tcggccacca
     1741 gttcggctcg ggccgaggca acgacgtgat gaccgccggc ggcgcggtgg ccggcggcgc
     1801 ggtgggctcg caggtgggcc gtggcggcgg cggcctggtc tacgacctcg ccatccgcat
     1861 ggacgacggc atcacccgcg tcatcaccgt ggccgcaccg ggcgagctgc gtaccggcag
     1921 ccgcgtgcgc atcgccggcg accacgtggt gccgatctga ttcctgctac ggaatccgta
     1981 gcagggcgca ggatcagtcc ggcaggctga cgccggatcc gcccatttcc ttggggaagt
     2041 ccttcatctt gggcaggccg tccttcacgc tcagcacctt ctcgccgtag ttgacgtgca
     2101 gcgccggctt gaacttgagt gaggggatga ccgcagcata tacgtcagtg aggcccatgc
     2161 ccggatgctc ggagaagatg tggccgccgc agtccttgca ccacttgcgg tagctgttct
     2221 cggtcttgtg ataggtgccg accttgtcgg cgcccctggt taccttcacc gccgacggct
     2281 gccacaggct gaaggcgttg accggcccgg ccgaccagtg gcggcacgac tcgcagtggc
     2341 agtaacccat cgccgccggt tcgccgctca ccgtgaactg caccgcgccg cagaagcagc
     2401 tg
//
LOCUS       DASZZC010000465         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_944211,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000465 DASZZC010000000
VERSION     DASZZC010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944211"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VHE37_09900"
     CDS             <1..457
                     /locus_tag="VHE37_09900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HVT35888.1"
                     /translation="LLKARFACLVSGLPAIADDSGFEIDALGGAPGVRSARWSGDNAT
                     DSSNNQMLLRELKGVPDEKRGARYVCVMVYLRSPEDPTPIVAQAIWRGTILHNPKGSG
                     GFGYDPLFYVPDQKMSAAEMDAGLKNRLSHRGKAAKHLYSLLHDAAGPL"
     gene            609..1742
                     /gene="hemW"
                     /locus_tag="VHE37_09905"
     CDS             609..1742
                     /gene="hemW"
                     /locus_tag="VHE37_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HVT35889.1"
                     /translation="MIPLSLYLHFPWCVRKCPYCDFNSHAARGEVPEGAYIDGLLRDL
                     DFELQQAEARELVSIFMGGGTPSLFSGAAIARLLDGVRARLMFARDIEITLEANPGTV
                     DEAHFAGYRAAGVNRLSIGVQSLDAEKLRALGRIHDPASARAAVRVARAAGFSNINLD
                     LMYALPQQTLAQAEADLRGLLELQPEHISYYQLTLEPNTEFAARPPPLPDDELAWDMQ
                     LQGQALLAQHGYAQYEVSAYARTGRRAKHNLNYWTFGDYLGIGAGAHGKRTLDGAIRR
                     RARHKHPKTYLEHAGLREAIQEERTVAAEDLPFEYAMNALRLNQGFELGDFSARTGAS
                     SQVLEPALARAQAAGLLERAGSRVLANELGRAHLNRLTQMFLP"
     gene            1753..1935
                     /locus_tag="VHE37_09910"
     CDS             1753..1935
                     /locus_tag="VHE37_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HVT35890.1"
                     /translation="MIEDVEVQCPYCGEALVLEIDTSAGSQRYTEDCSVCCQPMQVSV
                     RIDDGELAVEVVPENG"
     gene            1966..>2301
                     /locus_tag="VHE37_09915"
     CDS             1966..>2301
                     /locus_tag="VHE37_09915"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVT35891.1"
                     /translation="MDQPQPQVEYVGFWARVLAAIIDTIAVGLLLAVVSYVLGLRDLI
                     TLDAGGSPVFHAEALGDTAINRLLYAAVIVGFWSWKMATPGKMVINAVIVDASTFGKP
                     TLGQLIGRYL"
BASE COUNT          404 a          761 c          753 g          383 t
ORIGIN      
        1 gctgctcaag gcgcgctttg cctgcctggt ctccgggctg ccggcgatcg ccgatgattc
       61 cggcttcgag atcgacgcgc tcggcggcgc gcccggcgtg cgctcggcgc gctggagcgg
      121 cgacaacgcg acggactcgt cgaacaacca gatgctgctg cgcgaattga agggcgtgcc
      181 cgacgagaag cgtggcgcgc gctacgtgtg cgtgatggtt tacctgcgct cgccggaaga
      241 cccgacgccg atcgtggcgc aggccatctg gcgcggcacc atcctgcaca accccaaggg
      301 tagcggcggc ttcggctacg acccgctgtt ctacgtgccg gaccagaaga tgagcgcggc
      361 ggaaatggat gcgggcctga agaaccgcct gagccatcgc ggcaaggcgg ccaagcatct
      421 gtactcgctg ttgcatgacg ccgcggggcc gttgtagcgc gcgcagccgg tttttttcct
      481 ccaccgcacc ggcatggcgc gcgttattaa ttattatctg aaattaaaat cagacgataa
      541 ttagtaacac gtattcccag ggtgcggtga cggcaaaaaa gcgcgcgaac catcatgctg
      601 gcgctgccgt gattcctctt tcgctctacc tgcatttccc ctggtgcgtg cgcaaatgcc
      661 cgtactgcga cttcaactcg cacgccgcgc gcggcgaggt cccggaaggc gcgtacatcg
      721 acgggctgct gcgcgatctc gacttcgagc tgcagcaagc cgaagcgcgc gaactggttt
      781 cgatcttcat gggcggcggc acgcccagcc tgttctccgg cgcagccatt gcccgcctgc
      841 tcgatggcgt gcgtgcgcgg ctcatgttcg cgcgcgacat cgagatcacc ctggaagcca
      901 atcccggcac cgtggacgag gcgcacttcg ccggttaccg cgcagccggc gtcaaccgcc
      961 tgtccatcgg cgtgcagagc ctggacgcgg aaaaacttcg cgcgctcggg cgcatccacg
     1021 atcccgcatc cgcgcgcgcg gcggtgcgcg tggcgcgcgc ggcgggcttt tccaacatca
     1081 acctcgacct gatgtacgcg ctgccgcagc agacgctggc ccaggccgag gccgacctgc
     1141 gcggcctgct tgaactgcag cccgaacaca tcagctacta ccagctcacg ctggaaccga
     1201 acacggaatt cgccgcgcgc ccgccgccgc tgccggacga tgaactcgcc tgggacatgc
     1261 agctgcaggg ccaggcgctg ctcgcgcagc acggctacgc gcagtacgaa gtctccgcct
     1321 atgcgcgcac cgggcgccgc gcgaaacaca atctcaacta ctggaccttc ggcgactacc
     1381 tgggcatcgg cgcgggcgcg catggcaagc gcacgctgga tggcgccatc aggcgccgcg
     1441 cgcggcacaa gcatccgaaa acttatctcg agcacgccgg tctgcgtgaa gcgatccagg
     1501 aagaaagaac cgtggcggcg gaagacctgc cattcgagta cgcgatgaat gcgctgcgtc
     1561 tgaaccaggg tttcgagctg ggcgatttca gcgcgcgcac cggcgcatcg tcgcaggtgc
     1621 tcgaacccgc gctggcccgc gcccaggcgg cgggcctgct cgaacgcgca ggttcgcgcg
     1681 tgctcgccaa cgaacttggc cgcgcacacc tcaatcgtct cacccagatg ttcctgccct
     1741 gagcaagccg ccatgatcga agacgtcgag gtgcagtgcc cgtactgcgg cgaggcgctc
     1801 gtgctcgaga tcgacaccag cgccggcagc cagcgctaca ccgaggattg ttccgtgtgc
     1861 tgccagccga tgcaggtgag cgtgcgcatc gacgacggcg aactggcggt cgaagtcgtg
     1921 ccggaaaatg gctagagttc cggcgacaac atcagggggc agctcatgga tcagccgcag
     1981 ccgcaggtgg aatacgtcgg cttctgggca cgggtgctgg cagcgatcat cgacaccatc
     2041 gcggtcggcc tgctgctggc cgtggtcagc tacgtgctgg gtttgcgcga tctgatcacg
     2101 ctggatgccg gcggcagccc ggtgtttcac gctgaagcgc tgggcgacac ggcaatcaac
     2161 cgtctgcttt acgccgccgt gattgtcggc ttctggtcgt ggaagatggc cacgccgggc
     2221 aagatggtga tcaacgcggt gatcgttgat gccagcacgt tcggcaagcc gacgctgggc
     2281 cagctgatcg gccgctacct g
//
LOCUS       DASZZC010000466         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_99259,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000466 DASZZC010000000
VERSION     DASZZC010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99259"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2442)
                     /locus_tag="VHE37_09920"
     CDS             complement(<1..2442)
                     /locus_tag="VHE37_09920"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVT35892.1"
                     /translation="MRNHPWPALALLLLAGCGGGDGDGGQPGNGGGGAPPSTLSLKAE
                     NVLPPGQSGFISLAGQVQGTLTGNPGDYGAHVDDQRQMYWSFSAKPGVLGTKPGAVTH
                     PLARVDVYRDDAGVPIVYAANVHDLWFGVGYSVAQDRLFLMDAVRRMGAGTFGELTGC
                     GGVPADIQQRTLAYTDAEYQSFYDALSPDARDAVQGYVDGANAWRAAVMTDPTQLPAE
                     YALLTTTPAAFTVKDVLAGGVYITRFVAAEGGNEFLNIEMIKQLQAAYGSADAGLAAF
                     QDMTWLEDPKAVVTVPRSEGTFSNQAEPVAGRDAVFNSMATWALGLPETLWKGPGTGN
                     STVPAPCSQPSLPGPLGSAGGLGAGEVKFAAAPAPSKKGARRAAQIAELQRGVVRALA
                     QLRASLHGGSHAYAIAPSRTRDHGTLLVSGPQLGYTYPTLLVEYEIHGGGYDARGVSV
                     PILPVVGIGYSNDIAWGLTTGYSKTIDSFIETTCSTEQQAASACKANQYFHQGVWKDM
                     DCRGETFNYRAATQGVPVGPAVLSTTAQICRTVHGPIVARDDAAGFARSVQYAMYLHE
                     IDNIEGIRLWNRAHTFADFLDGARHLTWNENVVAATRDGHIAYIHPGRFPQRSPDTDM
                     RLPAPGTGQYDMGPMMAFEQLPHVFDPAQGFVANWNNKPAYGWLDGEGMGATSRPGGA
                     GQRVTTIMDAISARRDWRYDDLMALDTHVGTTDHRAREYLPVLASFRSASAASLSDVQ
                     RAALDLMLSWNGSHYGPNIDLSDASAHDAPAPTIFGEYVIALRDELFASLKNRIIDPG
                     VQDPDPNNPDPQAGLT"
     gene            2510..2980
                     /locus_tag="VHE37_09925"
     CDS             2510..2980
                     /locus_tag="VHE37_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HVT35893.1"
                     /translation="MAQIPLALRMWRRLSGKPGGKWIFSKAICQKAPYFSTINPRFAE
                     LAPGYCEVRCTNRRAVHNHIGTFHAIAMCNMAEVAGGTMTEVTVPATHRWIPKGMTVE
                     YLKKATTDLRAIAQLDPIPNFRGAADLPVTVNVYDARDQLVFRAVITMWVSPKR"
     gene            complement(2992..3696)
                     /locus_tag="VHE37_09930"
     CDS             complement(2992..3696)
                     /locus_tag="VHE37_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVT35894.1"
                     /translation="MIKLLLLLLKSKAVFTAGTMLLSMLAYSLVFGWWYAVGFVLLIF
                     FHEMGHFIAARQRGLNVGAPAFIPFVGAWIQLKDLPHNAEMEAYIGIAGPLAGTVASL
                     ACYYAARNWNSDLLLALSYSGFMINLFNLIPVSPLDGGRITAVISPRIWLIGAPILVG
                     FFLIYPSPMFILLALLAWPQLVAAWKGPGENAPYYDIPLGTRINYGGAYIALIAFLGQ
                     MTYSIHQMLGPYNGHH"
     gene            complement(3693..>4125)
                     /locus_tag="VHE37_09935"
     CDS             complement(3693..>4125)
                     /locus_tag="VHE37_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HVT35895.1"
                     /translation="LETTRKACERFRNQPALILNYLEGTRFTQAKHDRQQSPYRHLLK
                     PKSGGFAFTIAALGERLNSLLDITIVYPQGAMGFWDFLCGRMRRVIVEVRQLKIPAEF
                     FSGSYENDPEFRARFQKWIGELWSQKDQRIGQLLAQSGAAA"
BASE COUNT          727 a         1356 c         1350 g          692 t
ORIGIN      
        1 ggtgaggccg gcctgcggat cgggattgtt cggatcgggg tcctgcacgc ccggatcgat
       61 gatccggttc ttcaggctgg cgaacagttc gtcgcgcaac gcgatgacgt attcaccgaa
      121 tatcgtgggc gccggggcat cgtgcgcgga tgcatcggaa aggtcgatgt tggggccgta
      181 gtggctgcca ttccacgaca gcatcaggtc gagcgcggcc ctctgcacat ccgacagcga
      241 tgctgccgaa gcactgcgga agctggcaag gaccggcagg tattcgcgcg cacggtggtc
      301 agtggtgccg acatgcgtgt cgagcgccat gagatcgtcg tagcgccagt cgcggcgcgc
      361 agagatcgcg tccatgatgg tcgtcacgcg ctgacctgct ccacccgggc gcgaggtggc
      421 gcccatgcct tcgccatcca gccagccgta tgcaggcttg ttgttccagt tggcgacgaa
      481 cccctgcgcc gggtcgaaca cgtgcggcag ctgctcgaag gccatcatcg gccccatgtc
      541 gtactggccg gtgccgggtg ccggcaagcg catgtcggta tcggggctgc gctgcggaaa
      601 acgcccggga tggatatagg cgatgtgccc atcgcgcgtg gccgccacca cgttctcgtt
      661 ccaggtgaga tggcgcgcac cgtcgaggaa atcggcaaag gtatgcgcgc ggttccacag
      721 gcggatgcct tcgatgttgt cgatctcgtg caggtacatg gcgtactgca cgctgcgggc
      781 aaagccggcc gcatcgtcac gcgccacgat gggcccgtgc accgtgcggc agatctgtgc
      841 cgtggtcgac agcaccgccg gacccaccgg cacgccctgc gtggccgcgc ggtagttgaa
      901 ggtctcgccg cggcaatcca tgtctttcca cacgccctgg tggaaatact ggttggcctt
      961 gcacgcgctc gccgcctgct gctcagtcga gcaggtggtc tcgatgaagg agtcgatggt
     1021 cttgctgtag cccgtggtga ggccccaggc gatgtcgttg gaataaccga taccgacgac
     1081 gggcaggatc ggcaccgaca ctccgcgtgc atcgtatccg ccgccatgga tttcatattc
     1141 gaccagcagc gtgggatagg tgtagcccag ctgcgggccc gacaccagca gggtgccatg
     1201 atcgcgcgta cgcgacggcg cgatggcata agcatgcgag ccgccgtgca gcgatgcgcg
     1261 cagctgcgcc agagcccgta ccacgccgcg ctgcagctcc gcgatctgcg ccgcgcgccg
     1321 ggcacctttc ttcgacggcg ccggcgccgc tgcgaacttc acctcgcctg ctcccagccc
     1381 gcccgcactg cccagcggac ccggcaggct cggctgcgaa cagggcgctg ggaccgtgga
     1441 attgccggta cccggccctt tccagagggt ttcaggcaag cccagcgccc acgtcgccat
     1501 gctgttgaaa acagcatcgc gccccgctac cggttccgcc tgattggaaa acgtgccctc
     1561 gctgcgcggc acggtcacca ccgccttcgg gtcttccagc caggtcatgt cctggaacgc
     1621 cgccagaccc gcgtcggccg aaccgtaggc tgcctgcagc tgcttgatca tctcgatatt
     1681 gagaaactcg ttgccgccct cggccgcgac gaaccgcgtg atgtacacgc cgccggccag
     1741 cacgtccttg acggtgaatg cggccggcgt ggtcgtcagc agcgcgtact cggccggaag
     1801 ctgcgtagga tcggtcatca cggccgcgcg ccaggcgttg gcgccatcca cataaccctg
     1861 caccgcatcc cgggcatccg gggaaagcgc gtcgtagaac gactggtact cggcatcggt
     1921 gtaggccagc gtgcgctgct ggatatccgc cggcacacca ccgcaaccgg tcagttcgcc
     1981 gaaggtaccg gcgcccatgc ggcgcacggc atccatcagg aacaggcggt cctgcgcgac
     2041 ggaatagccg acgccgaacc agaggtcgtg cacgttcgct gcgtacacga tcggcactcc
     2101 ggcgtcgtcg cggtacacat cgacacgcgc cagcgggtgg gtgaccgcac cgggcttggt
     2161 gccgagcacg ccgggcttgg ccgaaaagct ccagtacatc tgacgctgat cgtcgacatg
     2221 cgcgccgtag tcgcccgggt ttccggtgag cgtgccctgc acctgtccag ccagggaaat
     2281 gaatcctgac tggccgggag gcagcacgtt ttccgctttg agcgaaagag tcgaaggagg
     2341 tgcgccgccc ccgccattgc cgggctggcc accgtcaccg tcaccgccac cgcagccggc
     2401 cagcagcaac agcgccagcg cgggccatgg atggtttctc atgtaaatct cctcgtcggc
     2461 ggcggcggcc acgcgctacc atgcgcaact ttttttggga gtttacccga tggcccagat
     2521 acctttggcg ctgcgcatgt ggcgccggct ctccggcaag ccgggcggca aatggatttt
     2581 ctccaaggcg atctgccaga aggcgccgta cttctccacc atcaatccgc gcttcgccga
     2641 acttgcaccc ggctattgtg aagtgcgctg caccaaccgg cgtgccgtgc acaaccacat
     2701 cggcaccttc cacgccatcg ccatgtgcaa catggcggaa gtcgccggcg gcaccatgac
     2761 cgaggtgaca gtaccggcca cgcaccgctg gattcccaag ggcatgaccg tggaatacct
     2821 gaagaaagcc accaccgacc tgcgcgcgat tgcgcagctg gatcccatcc cgaatttccg
     2881 cggcgccgcc gacctgccgg tgacggtgaa cgtctacgac gcgcgcgacc agctggtgtt
     2941 ccgcgccgtc atcaccatgt gggtgagccc gaagcgctga tccgtggatg atcagtggtg
     3001 gccgttgtac ggccccagca tctggtggat cgaataggtc atctgcccga ggaacgcgat
     3061 cagcgcaatg taggcgccgc cgtagttgat gcgcgtgccc agcgggatgt cgtagtacgg
     3121 cgcgttttct cccggccctt tccatgcggc caccagctgc ggccaggcga gcagcgccag
     3181 caggatgaac atcgggctcg ggtagatcag gaagaagccg accaggatcg gcgcgccgat
     3241 cagccagatg cgcggcgaga tcaccgcggt gatgcgcccg ccgtcgagcg gcgacaccgg
     3301 gatgagattg aacaggttga tcatgaagcc ggagtacgac aaagccagca gcaggtcgga
     3361 attccagttg cgcgccgcgt agtagcaggc cagcgaagca acggtgcccg ccagcgggcc
     3421 ggcgatgccg atgtacgctt ccatctccgc gttgtgcggc aggtccttga gctggatcca
     3481 cgcgcccacg aacgggatga aggccggcgc gccgacgttg agcccgcgct gccgcgccgc
     3541 gatgaagtgc cccatctcgt ggaagaagat cagcagcacg aagcccaccg cgtaccacca
     3601 gccgaatacc agcgagtacg ccagcatcga cagcagcatg gtgccggcgg tgaacaccgc
     3661 cttgctcttg agcaggagca agagcagttt gatcatgccg ccgctccgga ctgggccagc
     3721 agctgcccga tgcgctggtc cttctgcgac cacagctcgc cgatccattt ctggaagcgc
     3781 gcgcggaact cggggtcgtt ttcgtagctg ccgctgaaga actccgcggg aattttcagc
     3841 tggcgcactt ctacgatcac gcgccgcatg cggccgcaga gaaaatccca gaaacccatg
     3901 gcgccctgcg gatagacgat ggtgatgtcc agcagcgagt tgaggcgctc gcccagcgcg
     3961 gcgatggtga aggcaaatcc gccggacttg ggcttgagca ggtgccggta cggcgactgc
     4021 tgccggtcgt gcttggcctg ggtgaaacgc gtgccttcca ggtagttcag gatcagcgcc
     4081 ggctggttgc ggaaacgctc gcaggccttg cgtgtggtct ccagg
//
LOCUS       DASZZC010000467         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_489708,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000467 DASZZC010000000
VERSION     DASZZC010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489708"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VHE37_09940"
     CDS             complement(<1..781)
                     /locus_tag="VHE37_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35896.1"
                     /translation="MFTLIWINFKRDKGRKLLTLLSLLMAFVLFGVLMALREALTFDG
                     TGTAAARILVTLNEAGRGQPLPVAYAGRIGQLPGVIAVSYDSAALGAYQEPNNSFLFL
                     ATAGRGMLQVQPFLQIPPAQRRAWFQDRSGAIVTPKLMQKFGWKLGERVPVMTQVPRK
                     NGDTTWYVTIDGVVTDPTSHAALGLEKMFVHYSYFDQARAMGGGTVGSISERITPAHD
                     LGKVSRSIDALFTNAAPQTHTIPVNAVFRSIYGQIGNISLIV"
     gene            complement(786..1496)
                     /locus_tag="VHE37_09945"
     CDS             complement(786..1496)
                     /locus_tag="VHE37_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT35897.1"
                     /translation="MAAVAAQTSETVVSLKGVTKLYKRGSETLEVLKRLDLEVPRGDF
                     LALMGPSGSGKTTLMNLLGGLDRPTSGEVIVDGQDIGRLKDGALSAWRARHVGFVFQL
                     YHLLPVLTAAQNVALPLMLGGLGASERRKRAYAALTLVGLEERAEHRPAELSGGQEQR
                     VGIARAIVTDPALLLCDEPTGDLDRKTGDEILRLLQLLNSDHGKTIIMVTHDPHAAAK
                     AQRTLFMNKGVLAQEPEA"
     gene            complement(1605..2363)
                     /locus_tag="VHE37_09950"
     CDS             complement(1605..2363)
                     /locus_tag="VHE37_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVT35898.1"
                     /translation="MKIRTLVVDDMPLSRKRTRRYLTEVADVEIVGECGDGRQALAAI
                     DSLAPDLLLLDVQMPGMSGLALLEQVPVAQRPAVVFVTAFDEFAVPAFAAQAVDFLLK
                     PFDRERLDEVLGRVRAHLAAKRAQSNSPAVPAAASETVPSWLSRIAVKSVGRTLFVNV
                     DGVDWLETVGNYVCLHAGRDTHVVRATMNKLETQLDPAKFVRIHRSTMVRIEAIREVQ
                     PLFNGDRLVILHGGTRLTLSRSYRDKARAALGAL"
     gene            2350..>2552
                     /locus_tag="VHE37_09955"
     CDS             2350..>2552
                     /locus_tag="VHE37_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35899.1"
                     /translation="MRIFIEGIGFVKRNVQARGEARAGTGLQFQTGQAAIKIAQPCAC
                     VADADTLASSITSRSSSRRARNA"
BASE COUNT          453 a          839 c          819 g          441 t
ORIGIN      
        1 cgacgatcag gctgatgttg ccgatctgac cgtagatgct gcggaatacg gcattcacag
       61 ggatggtgtg agtctgcggc gcggcgttgg tgaacagcgc atcgatggag cgactgactt
      121 tcccgagatc gtgtgcgggc gtgatgcgtt ccgagatgct gcctaccgta ccccctccca
      181 tggcgcgcgc ctggtcgaaa taggagtagt gcacgaacat cttctcgagg ccgagcgcgg
      241 cgtggctggt cgggtcggta accactccgt caatggtcac ataccaggtg gtgtcgccgt
      301 tcttgcgcgg cacctgggtc atcaccggca ctcgctcgcc cagcttccag ccgaatttct
      361 gcatcagctt gggagtcacg atcgcgccgc tgcgatcctg gaaccaggct cgccgctggg
      421 cgggcgggat ctgcagaaac ggctggacct gaagcatccc acggccggct gtggccagaa
      481 acaggaagct gttgttcggc tcttgatatg cgcccagagc cgcggagtcg taactcactg
      541 cgatcacgcc tggcagttgg ccgatccgcc cggcatatgc aaccggcagg ggctggccgc
      601 ggccggcctc gttcagcgtg accagtatgc gcgcggcagc cgttccggtg ccatcgaagg
      661 tcagcgcctc cctcagggcc atcagcacgc cgaacaacac gaaggccatg agcagcgaca
      721 agagcgtcaa cagcttgcgt cccttgtcac gcttgaagtt gatccagatg agcgtgaaca
      781 ccggctcagg cctcgggttc ctgcgcgagc acgcccttgt tcatgaacag cgtgcgctgc
      841 gccttggcgg ccgcatgcgg atcgtgcgtc accatgatga tggtcttgcc gtgatcgcta
      901 ttgagcagct gcagcagccg caggatttca tcccccgtct tgcggtcgag gtcaccggtg
      961 ggctcgtcgc acaacagcag tgccggatcg gtgacgatgg cgcgcgcaat accgacccgc
     1021 tgttcctggc cgccggaaag ctcggcgggc ctgtgctcgg cgcgctcttc caggccgacc
     1081 agcgtgagcg cggcgtacgc gcgcttgcgg cgctcgctgg cgccgagtcc gccgagcatc
     1141 agcggcagcg ccacgttctg cgctgccgtc agcaccggca gcaggtgata aagctgaaac
     1201 acgaaaccga cgtggcgcgc ccgccaggcc gaaagtgcgc catccttcag cctgccgatg
     1261 tcctggccgt ccacgatgac ctcacccgag gtcggtcggt ccaacccacc cagcaggttc
     1321 atcagcgtcg tcttgccgga tccggacggc cccatcaatg ccaggaaatc gccgcgcggg
     1381 acttccagat ccaggcgctt cagaacctcc agcgtttccg agccgcgctt gtagagcttg
     1441 gtgacgccct tcaggctcac cacggtttcg gatgtttgcg cggcaacggc ggccatgatg
     1501 ctctccccgg atccataccc cgaatttaga aagacaccgg ccgagtccca atggaattgg
     1561 gacgaaccgt ggcgggcatg ggacgaattg cgggtatcgg gccttcaaag ggcgccgagc
     1621 gcggcgcgag ccttgtcacg gtaggaccga ctgagggtga gccgggtgcc gccgtgcaat
     1681 atcaccaggc gatcgccgtt gaacagcggc tggacctcgc ggatcgcctc gatgcgcacc
     1741 atggtcgagc ggtggatgcg cacgaacttg gccggatcga gctgggtttc cagcttgttc
     1801 atggtcgctc gcaccacatg cgtgtccctg ccggcatgca ggcacacgta attgcccacg
     1861 gtctcgagcc agtccacgcc gtcgacgttc acgaacaggg ttcggcccac ggatttcacc
     1921 gcgatgcggc tgagccagga gggcaccgtt tcggaagccg ccgcgggcac ggccggcgag
     1981 ttcgactgcg cccgcttcgc ggccagatgc gcccgcactc gtcccagcac ttcgtcgagg
     2041 cgctcacggt cgaagggctt gagcaggaaa tccacggctt gcgccgcgaa cgccggcacg
     2101 gcgaattcgt cgaaggcggt cacgaacacc accgccgggc gctgcgcgac aggaacctgc
     2161 tccaggagcg ccagaccgga catacccggc atttgcacgt ccagcagcag cagatccggt
     2221 gcgagcgaat cgattgccgc cagcgcctgt ctgccgtcgc cgcactcgcc cacgatctcg
     2281 acatccgcga cttccgtcag gtagcgacgc gtgcgcttac gcgacagcgg catgtcgtca
     2341 acgaccagcg tgcggatttt catcgaaggc ataggcttcg tgaaaaggaa tgtgcaggcg
     2401 cgcggtgagg cccgcgccgg gacgggtctt cagttccaga ccggccaggc cgccataaag
     2461 atagctcaac cgtgcgcgtg tgttgccgat gccgataccc tcgcgtcctc gatcaccagc
     2521 aggtcttctt cacggcgcgc gcgcaacgca ag
//
LOCUS       DASZZC010000468         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1132241,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000468 DASZZC010000000
VERSION     DASZZC010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132241"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VHE37_09960"
     CDS             <1..1070
                     /locus_tag="VHE37_09960"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HVT35900.1"
                     /translation="APRFLTSYYLMISLGGALAGVFAGVLAPRLFTGHHELPLALAFC
                     AALMLHATHNDAASRVARAAWFAGLLAALALCVQLAAQIAQQQRHARVLARNFYGVLS
                     VRDEPRGPQHDLRRVLMHGSIIHGEEFLDDARARQPITYYTSASGIGRALATLQPRGA
                     LRIGVVGLGAGTLAAYGRAGDVCRFYELNPQVIDIAQREFRFLSLSPAQIELVPGDAR
                     LSLQSEAPQQYDLLAVDAFSGDAIPVHLLTLQAFQLYLSHLKTAGVLALHLTNRHLDL
                     LPVAAAAAQRLHLAGVRIDTAPAADDSMTASTWVLLSRDAAVIAAAPIAAAAQPLPPP
                     ARAWTDDYSSAAGALKWRSGR"
     gene            complement(1123..>2687)
                     /locus_tag="VHE37_09965"
     CDS             complement(1123..>2687)
                     /locus_tag="VHE37_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35901.1"
                     /translation="NRLKAALAISAVIGAVAPAYSATNQYFLPYDVPTNGVKAKGLSV
                     VNASDFTDIRQIEAPTQVVSSPLVHFAGTPNATSQTYSNPHPSSLVYAKAGHWFFVNL
                     AASSTLKPARISSESGAQTVCNARIIDIDSSNPNAAWLLYQLPGTDAACNTSDDQFRA
                     AHLSDRGTVAPVTLSVTTLDDMYAVGDKYSLANSIVARDGTQVRMYNSALTSSQVVLD
                     GIANDSWGTAYATSDGNGEFIYVNGTLRELHDNGTLGNVVYTDPTGFGVQHFNNVDAN
                     NIYFLVHSFSSANTVKLYRVPRDGMSKSVLMYEGTVAAGADIDGQTTNTIILRLKNGA
                     TGQKLVAVSKTAVSGSPAPKVLETLTSGFIYFAAGNGGRVYYTTLTLSGSSYVSTAIS
                     KGEGGAGLVSQGAGSQWVGLAVSSAKNDGLLTYSYLALLTGKTGNNLQGATLSSFKLS
                     DSTVTPLTTFTAPVLDSFAFAFGSTGLGGMVTDGDSGQGDVFAVDFAAGRYAQITDTP
                     TVDEEVVSFDGG"
BASE COUNT          405 a          898 c          912 g          472 t
ORIGIN      
        1 cggcgccgcg tttccttacg tcgtactacc tgatgatctc gctcggcggc gcgctggctg
       61 gcgtgttcgc cggtgtgctg gcaccgcgcc tgttcaccgg ccaccacgag ttgccgctgg
      121 cactggcgtt ctgcgcagcg ctgatgctgc acgccacgca caacgatgca gcatcgcgcg
      181 tggcccgcgc cgcctggttc gccgggctgc tggcagcgct ggcgctgtgc gtgcagctgg
      241 ccgcgcagat cgcccagcag caacgccacg cgcgcgtgct ggcgcgcaac ttctacggcg
      301 tgctcagcgt gcgcgacgaa cctcgcgggc cgcagcacga tctgcgccgc gtgctgatgc
      361 acggcagcat catccacggt gaagagttcc tcgacgacgc gcgcgcacgg cagccgatca
      421 cctattacac gtcggccagc ggcatcggcc gcgcactggc aacgctgcag ccgcgcggcg
      481 ccttgcgcat cggcgtggtc gggctgggcg cgggcacgct ggcggcatac ggccgagccg
      541 gcgacgtctg ccgcttctat gaactgaatc cgcaggtgat cgacatcgcg cagcgcgagt
      601 tccgcttcct gtcgctgtcg ccggcgcaaa tcgagctggt tcccggcgat gcgcgcctgt
      661 ccctgcagtc cgaggcaccg cagcagtacg acctgctcgc ggtcgatgcg ttttccggtg
      721 atgccatccc ggtccatctg ctgacgctgc aagccttcca gctgtacctg tcgcatctca
      781 agaccgccgg cgtgctggcg ctgcacctga ccaatcgcca tctcgacctg ctgccggtgg
      841 ccgcggccgc cgcgcaacga ttgcaccttg cgggggtgcg catcgatacc gcgccggccg
      901 ccgacgactc gatgaccgca tccacctggg tgctgctgtc gcgcgacgcg gccgtgatcg
      961 ccgccgcgcc gatcgctgcc gccgcacagc cgctgccgcc gccggcacgc gcgtggaccg
     1021 acgactacag cagcgcggcg ggagcgctca agtggcgcag cggtcgctag cgcaaaaaaa
     1081 gggcccgccg aagcgggccc cgggaacaaa atttttccgc gatcagccgc cgtcgaaact
     1141 gaccacttcc tcgtccaccg tcggcgtgtc ggtgatctgc gcatagcgtc cagcggcgaa
     1201 gtcgaccgcg aagacgtcgc cctgcccgct gtcgccgtcg gtcaccatgc cgccaagtcc
     1261 ggtggaacca aaggcgaagg cgaacgagtc cagcaccggc gccgtgaacg tggtcagcgg
     1321 cgtgaccgtg ctgtcgctca gcttgaagct cgacagcgtg gcgccctgca ggttgttgcc
     1381 ggtcttgccg gtgagcagcg ccaggtagct gtaggtcagc aggccgtcgt tcttggctga
     1441 gctgacggcc agcccgaccc actgcgatcc ggcgccctgg ctgaccagcc cggccccgcc
     1501 ctcgcccttg ctgatcgcag tgctcacata ggaactgccg ctgagcgtca gcgtggtgta
     1561 gtacacgcgg ccgccgttgc cggcggcaaa gtagatgaag ccgctggtca gcgtctccag
     1621 caccttgggc gcgggcgagc cggacactgc ggtcttcgac acggccacca gcttttgtcc
     1681 ggtggcgccg ttcttcaacc gcaggatgat ggtgttggtg gtctggccgt cgatgtcggc
     1741 acccgcggcc accgtgccct cgtacatcag cacgctcttg gacatgccgt cacgcggtac
     1801 gcggtacagc ttgacggtat tggccgagct gaacgaatgc accaggaaat agatgttgtt
     1861 ggcgtcgacg ttgttgaaat gctgcacgcc gaagccggtg ggatcggtgt agaccacgtt
     1921 gcccagggtg ccgttgtcgt gcagctcgcg cagcgtgccg ttgacgtaga tgaactcgcc
     1981 gttgccgtcg ctggtggcat aggcggtgcc ccaggaatcg ttggcgatgc cgtcgagcac
     2041 cacctgcgag ctggtcagcg cggagttgta catgcgcacc tgggtgccgt cgcgggcgac
     2101 gatggaattg gccaggctgt acttgtcgcc gaccgcatac atgtcgtcca gcgtggtcac
     2161 cgacagcgtc accggcgcca ccgtgccgcg atcggacagg tgcgcggcgc ggaactggtc
     2221 gtcgctggtg ttgcacgccg cgtcagtgcc gggcagctgg tacagcagcc aggccgcgtt
     2281 cggattgctg gaatcgatgt cgatgatgcg cgcgttgcac accgtctgcg cgccgctctc
     2341 actggaaatg cgcgccggct tgagcgtgga gctcgccgcc aggttgacga agaaccagtg
     2401 tccggccttg gcatacacca ggctgctggg gtgcggattg ctgtaagtct ggctggtggc
     2461 attgggcgtg ccggcgaaat gcaccagcgg gctgctcacc acctgggtcg gcgcttcgat
     2521 ctggcggatg tcggtgaagt ccgaggcgtt gaccacggac aagcccttgg ccttcacgcc
     2581 gttggtgggc acgtcgtacg gcaggaagta ctggttggtg gcagagtacg cgggcgcgac
     2641 agcgccgatc acggcgctga tcgccagcgc ggctttcaat cgattca
//
LOCUS       DASZZC010000469         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1192819,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000469 DASZZC010000000
VERSION     DASZZC010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192819"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /gene="hisG"
                     /locus_tag="VHE37_09970"
     CDS             complement(<1..134)
                     /gene="hisG"
                     /locus_tag="VHE37_09970"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649297.1"
                     /note="short form of enzyme; requires HisZ for function;
                     catalyzes the formation of N'-5'-phosphoribosyl-ATP from
                     phosphoribosyl pyrophosphate; crucial role in histidine
                     biosynthesis; forms heteromultimer of HisG and HisZ;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HVT35902.1"
                     /translation="MTPLTLALSKGRILDDTLPLLARMGVTPAGDIGRLLRVPTNVPG
                     L"
     gene            complement(327..1307)
                     /locus_tag="VHE37_09975"
     CDS             complement(327..1307)
                     /locus_tag="VHE37_09975"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVT35903.1"
                     /translation="MKTRSTATLLASVLLAACSGGGSDNAAPGYGDAPAKSSMSADGL
                     TAIATLCQEELGSVLNWTDPSGEARSACLLTPPGATMATPLPMIVWLHPSLAPQDSVL
                     LTGLFTDRLTADLGGSATGFVLLLPAGRDTAHYYPVPDNVGLGWDNWYRNLDRSSPSL
                     NVDVAAIDHFIAAAVATGVVDTNRIYLSGWSNGAAMAQLYALNTPGIAAAAVYSSPDP
                     FSDVQDPGAQAPFASNLTPLMDIHNQCDIIGICQTGTAFHQHLATRYPGLVQNPVIIS
                     ELFQQVDACDASCAGQDMINEASPGTVNHLRWPVTWNAQMYAFMKAHPLH"
     gene            complement(1479..2744)
                     /gene="murA"
                     /locus_tag="VHE37_09980"
     CDS             complement(1479..2744)
                     /gene="murA"
                     /locus_tag="VHE37_09980"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HVT35904.1"
                     /translation="MDKFVIKGNGPLKGEVAASGAKNAALPIMCAALLSDAPLHLTNV
                     PKLWDIGTCRKLLAQMGVSVEEPKPHELIINAKPIKTHIAPYELVRTMRASILVLGPL
                     VARRGEAHVSLPGGCAIGARPVDLHLMGLKAMGAEIKVEGGFVHAKAKKLKGARIMFD
                     TVTVTGTENLMMAATLADGETVLENAAREPEVVDLARCLRAMGAKIEGDGTTTIRIKG
                     VKKLHAGEHNILPDRIETGTFLVAGAVTRGKVKVTHTDPALLDAVLVKLQQAGAVIRT
                     GNDFIELDMKGQQPKAVNIHTMPHPGFPTDMQAQFMALDCLADGVGVIKETIFENRFM
                     HAQELLRLTADIRLEGNTAVITGKERLIAAPIMATDLRASASLVIAAMGADGETFIDR
                     IYHMDRGYEDIEGKLAALGARIKRVSKLL"
     gene            complement(2893..3126)
                     /locus_tag="VHE37_09985"
     CDS             complement(2893..3126)
                     /locus_tag="VHE37_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HVT35905.1"
                     /translation="MNAKDIEKLIEAGLPGSVAQVKGDDGRHFEALVLCPGFEGKSLV
                     QQHRMVYATLGDRMGGEIHALALTTRAPKAPCA"
     gene            complement(3123..>3474)
                     /locus_tag="VHE37_09990"
     CDS             complement(3123..>3474)
                     /locus_tag="VHE37_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT35906.1"
                     /translation="MLLAMFLLAAASLAVTGLIAGIVALKFDHIASFTNFFIMPLSFL
                     SGVFYSIHTLPPFWRAVSHGNPFFYMIDGFRYGFFGVSDTSPALALGWVAGFLALAIG
                     ICLWMLRTGYRLRP"
BASE COUNT          571 a         1101 c         1177 g          625 t
ORIGIN      
        1 aggcccggca cattggtcgg cacgcgcagc aggcggccga tgtcgccggc gggcgtcacg
       61 cccatgcggg cgagcagcgg cagcgtatcg tcgaggatgc ggcctttgga cagcgcgagc
      121 gtgagcgggg tcatggggcg cgggattcta cagaacgcgc cgcgccccgc ccacttgcaa
      181 gatcagcaac ctatgacaag cgtcgcgagc gaaggcagtt cgcgcgagga cggagcgcag
      241 gaaccgcagc atacgttaaa tgtatgtgag gattccgagc accgccctcg cgcaaaatgc
      301 cgagcgcagc agctttgcag aggatgctag tgaagtggat gcgccttcat gaatgcatac
      361 atctgcgcat tccaggtgac cggccagcgc aggtggttca ccgtgccggg ggaggcttcg
      421 ttgatcatgt cctggccggc gcaggaggcg tcgcaggcat ccacctgctg gaacagctcg
      481 ctgatgatca ccgggttctg caccaggccc gggtagcgcg ttgccaggtg ctggtggaac
      541 gcggtgcccg tctggcagat gccgatgatg tcgcactggt tgtggatgtc catcagcggc
      601 gtgaggttgc tggcgaacgg cgcctgcgcg cccggatcct gcacgtcgga aaacggatcg
      661 ggcgaggaat acaccgccgc tgccgcgatg ccaggcgtgt tcagcgcgta gagctgcgcc
      721 atggccgcgc cgttggacca gccggacaga tagatgcgat tggtgtcgac cacgccggtg
      781 gcgaccgcgg cggcgatgaa gtgatcgatg gccgccacgt cgacattgag cgacgggctg
      841 ctgcgatcga ggttgcgata ccagttgtcc cagcccaggc ccacgttgtc cggcaccggg
      901 tagtagtgcg cggtgtcgcg gccggcgggc agcagcagga cgaagccggt ggcgctgccg
      961 ccgagatcgg cggtgaggcg gtcggtgaac aggccggtga gcagcacgct gtcctgcggc
     1021 gccagcgaag gatgcagcca cacgatcatc ggcagcggcg tggccatggt cgcgccgggc
     1081 ggagtcagca ggcaggcgct gcgcgcttcg cccgacggat cggtccagtt gagcacgctg
     1141 cccagttctt cctggcacag cgtggcaatg gcggtgaggc cgtcggccga catgctcgat
     1201 ttggccggcg catcgccgta gcccggcgcg gcgttgtcgc tgccgccacc gctgcatgcg
     1261 gcgagcagga cgctcgccag tagtgtcgca gtcgatctgg ttttcatcgg cgctctcccc
     1321 gctgtgcgag gcgattatag ggagcgcggc acgcgaaacc tgtgcgattt ttgcccgacc
     1381 gcactgcctc gccacgtgtt actaaatatt gtttattttt aaattaatac aacaattaat
     1441 aacaccgaaa atgccggtgc ggtggaggct ttttgggctc agagcagctt gctcacgcgc
     1501 ttgatcctgg cgccgagcgc agcgagcttg ccctcgatgt cctcgtagcc gcggtccatg
     1561 tggtagatgc ggtcgatgaa ggtttcgccg tccgcgccca tcgccgcgat gacgagtgaa
     1621 gcgctggcgc gcaagtccgt cgccatgatc ggcgcggcga tcagccgctc cttgccggtg
     1681 atcaccgcgg tgttgccttc caggcggatg tcagcggtca ggcgcagcag ttcctgcgcg
     1741 tgcatgaagc ggttctcgaa gatggtttcc ttgatcacgc ccacgccatc ggccaggcaa
     1801 tccagcgcca tgaactgcgc ctgcatgtcg gtggggaaac ccgggtgcgg catggtgtgg
     1861 atgttcaccg ccttgggctg ctggcccttc atgtccagct cgatgaaatc gttgccggtg
     1921 cggatcaccg cgccggcctg ctgcagcttg accagcactg cgtcgagcag cgcgggatcg
     1981 gtgtgcgtca ccttgacctt gccgcgcgtc accgcgccgg cgacgagaaa cgtgccggtc
     2041 tcgatgcggt cgggcaggat gttgtgctcg ccggcgtgca gcttcttcac gcccttgatg
     2101 cggatggtcg tggtgccgtc gccctcgatc ttcgcgccca tcgcgcgcag gcagcgcgcg
     2161 aggtcgacga cttccggttc gcgcgcggcg ttctccagca cggtttcgcc gtcggccagc
     2221 gtagccgcca tcatcaggtt ctcggtgccg gtcaccgtca ccgtgtcgaa catgatgcgc
     2281 gcgcccttca gctttttcgc cttggcgtgg acgaagccgc cctccacctt gatctccgcg
     2341 cccatggcct tgaggcccat caggtgcagg tcgaccgggc gcgcgccgat ggcgcagccg
     2401 ccgggcagcg acacgtgcgc ctcgccgcga cgcgccacca gcgggcccag caccaggatg
     2461 ctcgcgcgca tggtgcgcac cagctcgtag ggtgcgatat gcgttttgat cggcttggcg
     2521 ttgatgatga gttcgtgcgg cttgggctct tccacgctca cgcccatctg cgccagcagc
     2581 ttcctgcagg tgccgatgtc ccacagcttg ggcacgttgg tgagatgcag cggcgcgtcg
     2641 gacagcagcg ccgcgcacat gatcggcagc gcggcgttct tggcgccgct ggcggcgact
     2701 tcgcccttga gcgggccgtt gcccttgatg acgaatttgt ccacgtttta ttcccgtcat
     2761 tcccgcgaag gcgggaatcc agtcctcaag ctagatgatc gttgtgcagg tcgtgtgcaa
     2821 atgccgcggg atttttccca tgcggaaaaa accggcttcc cgcctgcgcg ggaatgactg
     2881 ttcttcacgt catcacgcgc acggcgcctt gggcgcgcgc gtggtcagcg ccagcgcgtg
     2941 gatctcgccg cccatgcggt cgcccagcgt ggcgtagacc atgcggtgct gctgcaccag
     3001 cgacttgccc tcgaaacccg ggcacagcac cagcgcctcg aagtggcggc cgtcgtcgcc
     3061 cttcacctgc gccacgctgc cgggcagccc cgcctcgatc agtttctcga tgtcttttgc
     3121 gttcatggtc tcagccggta gccggtgcgc agcatccaca ggcaaattcc tatcgccagc
     3181 gcgagaaagc ccgccaccca gcccagcgcc agtgcgggcg aggtgtccga cacgccgaag
     3241 aagccgtagc ggaagccatc gatcatgtag aagaacggat tgccgtgcga caccgcgcgc
     3301 cagaacggcg gcagcgtgtg gatcgagtag aacacgccgg agaggaacga cagcggcatg
     3361 atgaagaagt tggtgaacga ggcgatgtgg tcgaacttca gcgcgacgat gccggcgatc
     3421 agcccggtca ccgccaggct cgccgccgcc agcaggaaca ttgccagcag catg
//
LOCUS       DASZZC010000470         7722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_167800,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000470 DASZZC010000000
VERSION     DASZZC010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167800"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VHE37_09995"
     CDS             complement(<1..1214)
                     /locus_tag="VHE37_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT35907.1"
                     /translation="MHDVRAKDGIVPAAAPSPAVKLPLHTKFSLGLGATAFGVKDQGF
                     NTLLMLYYNQVIGLPAAWVGGAIMLAMLVDAVADPLIGHWSDGLRSRWGRRHPFMYAS
                     ALPIGLAYFLLWSPPEASHAVQFAYLLATAVSVRFAIACFEIPASALITEFTSDYDER
                     TSLSSYRSVFYALGFVGMAIVTYKTVMQGGGDHQVGQLNAAGYIRYGWIAGPLMLVCV
                     LLGALGTHDRIPTLPLAAAGGHRGLAGMAGDMRAIFFDRTLASLLLCAFFFAVVGGLS
                     TTLGTYMYTYFWRFDAPQLATISGAGVWGMILGVLIAQLSRRYGKKLIATILYSGAIV
                     TFTAPILAALLGLLPRDPAVALIFLAAQNALMITCVLGGLVLVTSMLADVGDYFQLKT
                     GQRMEGLMFAAL"
     gene            complement(1214..3661)
                     /locus_tag="VHE37_10000"
     CDS             complement(1214..3661)
                     /locus_tag="VHE37_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35908.1"
                     /translation="MPDRMARCIIACTLLSAAMSGAYAQPTDGSADAAPPQDAPAQQA
                     APPADAADAATAVTPPSTEAAAEPPAPVQEAQAATIPVTELHPAPAQEAQAAEEAPHR
                     LEDVVVTATKRTKSLRDLPQSISAFDGETLERKGKMNLSDYIQETPGVVANDYGSGLV
                     QVTMRGITTDTSPLSPIPSAVGFFIGDTSFTDPYINGVTPDLSAFDLADVEVLKGPQG
                     TLFGGAALAGAIRYQLQEPVIGEWQAKGFGQYVTIDQGSPAWTEGGALNVPLWTDHDL
                     AARVVYVKRNYGGVYDDFRSGQKDVNRGGGDQVRATLLWQPADWKFKLTHLTQDFSTP
                     NGANTTLSPDGPRGSGAFLYPEPASNSFSLDTFEGNYNFDAMRLVSLTSYSTKKLQVY
                     ADATAVLVGVPPPNYPASAGAFETFYDNSRSIAQELRLQSTSSGDFQWLVGAYLFRYQ
                     VNFDLVADTVANQALHQGLGGLFDPVYDDTVLIHAYTDAKAREDALFFDLSEKFWDRL
                     ELSGGARLYRTGVSGGFEGNGVLARAANNGQNVNALHNAIDEKGINPRLAATFHFSRD
                     ISLYAQAAKGFRFGGVQSVPSSPTNGVPATYQSDSLWNYELGVRTAWLDNTLHADLTG
                     FYIQYRNPQIVQKTQTTSLNFTSNVPGAVSRGLEASVLWLTPLNGLRVSLSGGVDDTY
                     TTKPFKAVTGDTVEPGTQMPGTAKSQYNADLLYVLPVSFMAAGADLGYTYVGKGYSDL
                     LETVPVNDFGTLNATLYGGMQLGSLRPQLSISMSNILNVTTPIAGGSGKSITDIPFTT
                     YTLNPPRALTLRLSLDF"
     gene            3750..4511
                     /locus_tag="VHE37_10005"
     CDS             3750..4511
                     /locus_tag="VHE37_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVT35909.1"
                     /translation="MRTLHEPILDTIRHWRWFQYVPAQAHAWLASRASVQTIAQGTLL
                     YSPGDPVKAIYGVIDGVFRIYSALPKGEDLTLEEVVAGGWFSHMIPDPRATQITHCVC
                     QQDARVVVIPWSTVCEFGRRWPDFYRGCYEEFLLRAGVIIGRLELLSAHDLKVRLAVY
                     LLRIAKLRGVRAPDGSIWLGSAANQSEVGARVGGARQRVNYALKEWSREGWLTLGKDG
                     IRLLDLQAIRAQATRTGFDVDAYLGGWHGGWQGSS"
     gene            4589..6157
                     /locus_tag="VHE37_10010"
     CDS             4589..6157
                     /locus_tag="VHE37_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVT35910.1"
                     /translation="MPVVRVESGALLGSEDSGICSFKGVPYAEPVGAAARWLPPVPRK
                     PWSGMRDAVLDGPACAQFAARAGIALPGLRRKYFDAAGGNSFVREGDDCLLLDICTPS
                     PERGARLPVMVYFHGGGFTGGGATFYPGRNFAARNVVYVAVQYRLGPPGFLHGSGLFD
                     GDFCADNRAFLDQLQALKWVQENIAAFGGDAGNVTIFGESAGAFSVFQLCASPLGKGL
                     FRRAIAMGGMPFTSAPAQDYHRLTADLLSAAGVKPGDKQALIAMDLPALRRLQGAITR
                     TVFSRAAAERYGILGWSKVGFVGAAIGSGFLDASALTALRRGTPNNIDLLLGTCADDG
                     QLFSLFLPFSRTLSARMFMNNLAGLAPGGDLAALIERYRALLPRLPRSQLYDKLNNDL
                     FYRIPTIVAAEAHAAAHAGRTWHYELDLQSGIAGLRAIHGIDVGLLFGSECAASALLP
                     AGDAAADVSKKMLDCWVSFATTGRPSAAGMPAWNAYDSATRTSMVFDRDTRSEQDRGS
                     DLRRLWDGYRWERL"
     gene            complement(6165..6302)
                     /locus_tag="VHE37_10015"
     CDS             complement(6165..6302)
                     /locus_tag="VHE37_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35911.1"
                     /translation="MLIAKRIAIALAVIALLAGGWLLVRRYDPAPPQICLDGSCHDAN
                     N"
     gene            complement(6543..>7722)
                     /locus_tag="VHE37_10020"
     CDS             complement(6543..>7722)
                     /locus_tag="VHE37_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT35912.1"
                     /translation="TLGSLGCAASRGIGALVAARVLQGLGGAMMVPVARLVLVRQMPR
                     SDLVSAMATVTIPALVAPILGPPVGGLIVTYASWPWIFLINLPIGVLGWQLVRRHVGN
                     ARDSAHAPLDLAGWWLLGGGLAGLVFGFENIGKRVLPQDVSTGVLAAGALLLALYVRH
                     ARAERWPILRLSLLRVETFRASVAGGSLFRIGVGSFTLLLPMMLQLGYGLSTLHSGLL
                     TFASALGALGMKTIAQRMTRRYGFRPLLVWNSLACATALAACGWLRPQWPAEIIIVFL
                     AATGALRSLQFTCINALAFADVAERDMSQATSFSAAAQQLALTIGVGIGSQVLNASLA
                     LRHGSELAPRDFTAAFCVVALLTLASSFAFSRLSADAGSAVSGHRLALATEPVTSAAD
                     "
BASE COUNT         1208 a         2594 c         2725 g         1195 t
ORIGIN      
        1 atcagcgcgg caaacatcag gccttccatg cgctggccgg tcttcagctg gaagtagtcg
       61 ccgacgtcgg ccagcatcga ggtgaccagc accaggccgc cgagcacgca ggtgatcatc
      121 agcgcgttct gcgcggcgag gaagatcagc gcaaccgccg gatcgcgcgg cagcaggccg
      181 agcagcgcag cgaggatcgg cgcggtgaag gtgacgatgg cgccgctgta caggatggtg
      241 gcgatgagct ttttcccgta gcggcgcgac agctgcgcga tcagcacgcc caggatcatg
      301 ccccacacgc cggcgccgga aatggtggcc agctgcggtg catcgaagcg ccagaagtag
      361 gtgtacatgt aggtgcccag cgtggtgctc agcccgccga ccacggcgaa gaagaacgcg
      421 cacaggagca ggctggccag cgtgcggtcg aagaagatcg cgcgcatgtc gccggccatg
      481 ccggccagtc cgcgatgacc gccggcggcc gccagcggca gcgtggggat gcggtcgtgc
      541 gtgcccagcg cgccgagcag cacgcagacc agcatcagcg ggccggcgat ccagccgtag
      601 cggatatagc cggcggcatt gagctggccg acctggtgat cgccgccgcc ctgcatcacc
      661 gtcttgtagg tgacgatggc catgccgacg aagcccagcg cgtagaacac gctgcgatag
      721 ctggacaggc tggtgcgctc gtcgtagtcg ctggtgaact cggtgatcag cgccgaggcc
      781 gggatctcga agcaggcaat ggcgaaacgc acgctgaccg cggtggccag caggtaggcg
      841 aactgcacgg cgtggctggc ttccggcggc gaccacagca ggaaataggc gagcccgatc
      901 ggcagtgccg aggcatacat gaacggatgg cgccggcccc agcgcgagcg caggccgtcg
      961 ctccagtggc cgatcagcgg atcggccacg gcatcgacca gcatggcgag catgatcgcg
     1021 ccgccgaccc acgccgccgg caggccgatc acctggttgt agtacagcat cagcagcgtg
     1081 ttgaagccct ggtccttgac gccgaacgcg gtggcgccca ggcccagcga gaacttggtg
     1141 tgcagcggca gcttcaccgc cggcgacgga gcggccgccg ggacgatccc atccttcgcg
     1201 cgcacgtcgt gcatcagaag tccaggctca ggcgcagcgt gagcgcgcgc ggcgggttga
     1261 gcgtgtaggt agtgaacggg atgtcggtga tcgacttgcc cgagccgccg gcgatcggcg
     1321 tggtgacgtt gaggatgttg ctcatgctga tcgacagctg cggccgcagg ctgcccagct
     1381 gcatgccgcc gtacagggtg gcgttgagcg tgccgaaatc gttgaccggc acggtttcca
     1441 gcaggtcact gtagcccttg cccacgtagg tgtagccgag gtcggcgccg gcggccatga
     1501 agctcaccgg cagcacgtag agcaggtcgg cgttgtactg gctcttggcg gtgcccggca
     1561 tctgcgtccc tggctcgacc gtatcgccgg tgacggcctt gaacggcttg gtggtgtagg
     1621 tgtcatcgac cccgccgctg agcgaaaccc tgagcccgtt gagcggcgtc agccacagca
     1681 cgctggcctc caggccgcgg ctgaccgcgc cgggcacgtt gctggtgaag ttcaggctgg
     1741 tggtctgcgt cttctgcacg atctgcggat tcctgtactg gatgtagaag ccggtgaggt
     1801 cggcgtgcag cgtgttgtcg agccaggccg tgcgtacgcc gagctcgtag ttccacaggc
     1861 tatccgactg gtaggtcgcc ggcacgccgt tggtgggtga cgatggcacg ctctgcacgc
     1921 cgccgaagcg gaagcccttg gccgcctgcg cgtagagcga gatgtcgcgg ctgaaatgga
     1981 acgtggcggc cagccgcgga ttgatgccct tctcgtcaat ggcgttgtgc agggcattca
     2041 cgttctgccc gttgttggcg gcgcgcgcca gcacgccgtt gccttcaaag ccgccgctga
     2101 cgccggtgcg gtacaggcgc gcgccgccgg acagctccag gcgatcccag aatttttccg
     2161 acaggtcgaa gaacagcgcg tcttcgcgcg ccttggcgtc ggtgtaggcg tggatcagca
     2221 cggtgtcgtc gtagaccggg tcgaacaggc cgcccaggcc ctggtgcagc gcctggttgg
     2281 ccacggtgtc ggccaccagg tcgaagttca cctggtagcg gaacaggtag gcgccgacca
     2341 gccactggaa gtcgccgctg ctggtcgact gcagtcgcag ctcctgcgcg atgctgcgcg
     2401 agttgtcgta gaaggtctcg aacgcgccgg ccgacgccgg ataattgggc ggcggcacgc
     2461 cgaccagcac cgcggtggcg tcggcgtaca cctgcagctt cttggtcgag tacgaggtga
     2521 gcgacaccag gcgcatggcg tcgaagttgt agttgccttc gaaggtgtcg aggctgaagc
     2581 tgttgctggc cggttccggg tacaggaacg cgccgctgcc gcgcggacca tccgggctga
     2641 gcgtggtgtt ggcgccgttg ggcgtggaga aatcctgcgt caggtgcgtg agcttgaact
     2701 tccagtcggc cggctgccac agcagcgtgg cgcgcacctg gtcgccgccg ccgcggttga
     2761 cgtctttctg gccgctgcgg aaatcgtcgt agacgccgcc gtagttgcgc ttgacgtaga
     2821 ccacgcgcgc ggccaggtcg tggtcggtcc acagcggcac gttcagcgcg ccgccctcgg
     2881 tccatgcggg cgagccctgg tctatggtca cgtactgtcc aaagcccttg gcctgccatt
     2941 cgccgatcac cggctcctgc agctggtagc ggatggcgcc ggccagcgcg gcgccgccga
     3001 acagcgtgcc ctgcgggccc ttgagcactt ccacgtcggc caggtcgaaa gccgacaggt
     3061 cgggcgtcac gccattgatg tacggatcgg tgaagctggt gtcgccgatg aagaatccga
     3121 ccgcgctggg gatcggcgac agcgggctgg tgtcggtggt gatgccgcgc atggtcacct
     3181 gcaccagccc ggagccgtag tcgttggcca ccacgccggg cgtttcctgg atgtagtcgg
     3241 acaggttcat cttgcccttg cgctccagcg tctcgccgtc gaacgcgctg atcgactgcg
     3301 gcaggtcgcg cagcgacttg gttcgcttgg tcgcggtgac caccacgtcc tcgaggcggt
     3361 gtggagcttc ctccgcagcc tgcgcttcct gcgccggcgc cgggtgcagt tcggtgaccg
     3421 ggatggtggc ggcctgcgct tcctgcaccg gcgcaggcgg ttcggccgcc gcttcggtgg
     3481 agggcggcgt caccgcagtt gccgcatcgg cggcatccgc gggcggcgcg gcttgctgcg
     3541 cgggcgcgtc ctgcggcggg gcggcgtccg cacttccgtc cgtcggttgc gcgtacgcgc
     3601 ccgacatcgc ggcagagagc agggtacagg cgatgatgca gcgagccatt ctgtctggca
     3661 tttatgtttc ctccagccgt acgagtgtgc gcatgccgcg ccgccgctgt ctgtcacaac
     3721 tgggacagcc tcgacataaa attccgtcga tgcgcaccct ccacgaaccg atcctcgaca
     3781 ccatccgcca ctggcgctgg ttccagtacg tgccggcaca ggcgcatgcc tggctcgcct
     3841 cgcgtgccag cgtgcagacc atcgcgcagg gcacgctgct gtattcgccg ggtgacccgg
     3901 tgaaggccat ctacggcgtg atcgatggcg tgttccgcat ctacagcgcg ctgcccaagg
     3961 gcgaagacct gacgctggag gaagtggtgg ccggcggctg gttcagccac atgattcccg
     4021 accccagggc cacgcagatc acccactgcg tgtgccagca ggatgcgcgc gtggtggtga
     4081 tcccgtggag cacggtgtgc gagttcggcc gccggtggcc ggatttctac cgcggctgct
     4141 acgaggaatt cctgctgcgc gccggcgtca tcatcggccg gctcgagctg ctcagcgcgc
     4201 acgacctcaa ggtgcgcctg gcggtctacc tgctgcgcat cgccaagctg cgcggcgtgc
     4261 gcgcgcccga cgggtccatc tggctgggca gcgcggccaa ccagtccgag gtcggcgcgc
     4321 gcgtgggcgg cgcgcgccag cgcgtgaact acgcgctcaa ggaatggagc cgcgaaggct
     4381 ggctcacgct gggcaaggac ggcattcgcc tgctcgacct gcaggccatc cgcgcgcagg
     4441 ccacgcgcac cggcttcgat gtcgacgcct acctcggcgg ctggcacggc ggctggcagg
     4501 gctcgtcgta acgcgcttgt ctcactggtg acagcggttt gcacggcact tgcttagcat
     4561 ccggcggatg acaccaggaa aaagcagcat gccggtcgtg cgggtggaaa gcggggcgct
     4621 gctcggcagc gaggacagcg gcatctgctc gttcaagggc gtgccgtacg ccgagccggt
     4681 gggcgctgcc gcgcgctggc tgccgccggt gccgcgcaaa ccctggagcg gcatgcgcga
     4741 cgccgtgctc gacggccccg cctgcgcgca gttcgccgcg cgcgccggca tcgcgcttcc
     4801 cggactgcgc aggaaatatt tcgacgccgc cggcggcaat tcgtttgtgc gggaaggcga
     4861 cgactgcctg ctgctcgaca tctgcacgcc gtcgccggag cgcggcgcgc gcctgccggt
     4921 gatggtgtac ttccacggcg gcggcttcac cggcggcggc gcgaccttct acccggggcg
     4981 caatttcgcc gcgcgcaacg tggtctacgt ggccgtgcag taccggctcg ggccgccggg
     5041 cttcctccac ggcagcggcc tgttcgacgg cgacttctgc gccgacaacc gcgccttcct
     5101 cgaccagctg caggcgctga agtgggtgca ggaaaacatc gcggccttcg gcggcgacgc
     5161 gggcaacgtc accatcttcg gcgaatcggc cggcgcgttc agcgtgttcc agctgtgcgc
     5221 cagcccgctg ggcaagggat tgttccggcg cgccatcgcc atgggcggca tgccgttcac
     5281 ctctgcgccg gcccaggact accaccggct caccgccgac ctgctgagcg cggccggcgt
     5341 gaagcccggc gacaagcagg cgctgatcgc catggacctg ccggcgctgc gccggctgca
     5401 gggcgccatc acccgcaccg tgttcagccg cgcggcggcg gaacgctacg gcatcctcgg
     5461 ctggagcaag gtgggattcg tcggcgccgc catcggcagc ggcttcctcg acgccagcgc
     5521 gctgaccgcg ctgcgccgcg gcacgcccaa caacatcgac ctgctgctcg gcacctgcgc
     5581 cgacgacggc cagctgttct cgctgttcct gccgttctcg cgcacgctgt cggcgcgcat
     5641 gttcatgaac aatctggccg gcctggcgcc cggcggcgat ctggccgcgc tgatcgaacg
     5701 ctaccgcgcg ctgctgccgc gcctgccgcg gagccagctc tacgacaagc tcaacaacga
     5761 cctgttctac cgcatcccga ccatcgtggc ggcggaagcg cacgcggccg cgcatgcggg
     5821 ccgcacctgg cattacgagc tggatttgca gtcgggcatt gccggcctgc gcgcgatcca
     5881 cggcatcgac gtcggcctgc tgttcggcag cgaatgcgcc gcgtcggcac tgctgccggc
     5941 cggagacgcg gccgccgacg tatcgaaaaa aatgctcgac tgctgggtga gtttcgccac
     6001 caccggccgc ccgtcagcgg cgggcatgcc ggcgtggaac gcctacgaca gcgccacgcg
     6061 cacgtccatg gtgttcgacc gcgacacgcg cagcgagcag gaccgcggca gcgatctgcg
     6121 ccgcctctgg gatggctacc gctgggagcg tctctgacgc gcgctcagtt gttggcgtcg
     6181 tggcagctgc cgtccaggca gatctgcggc ggcgcgggat cgtagcgccg aaccagcagc
     6241 cagccgcccg cgagcagcgc gatgaccgcg agtgcaatgg cgatgcgttt ggcgatgagc
     6301 atggcgcgat gctagcgccg cgggattcgc gcacgcgcat cgcgggatcg gcggcgatga
     6361 accgcccgac atacggaatg gaaaccgcca ggacgaaccg cgcacaggct tttttgccgc
     6421 caccgcactg ccatctggtg tgttataaat aataatatat ttttatattt gattgatatt
     6481 ttataacatc atttccagca gtgcggtgag ctggtttcgg gcggttccgg atccgccgcc
     6541 gatcagtccg ccgcgctggt aaccggctcg gtggccaggg ccagccggtg tccgctcacc
     6601 gcgctgcccg cgtccgccga cagccgggag aacgcgaacg acgatgccag cgtgagcaac
     6661 gcgaccacgc agaacgccgc ggtgaaatcg cgcggcgcca gctcgctgcc gtggcgcagc
     6721 gcgaggctgg cgttgagcac ctgcgagccg atgcccacgc cgatggtcag cgccagctgc
     6781 tgcgcggcgg cggagaaact ggtggcctgg ctcatgtcgc gctcggccac gtcggcgaag
     6841 gccagcgcgt tgatgcaggt gaactgcagc gagcgcagcg cgccggtggc ggcgagaaac
     6901 acgatgatga tttccgccgg ccactgcggg cgcagccagc cgcaggcggc cagcgcggtg
     6961 gcgcaggcca ggctgttcca caccagcagc gggcggaagc cgtagcgccg cgtcatgcgc
     7021 tgcgcaatgg tcttcatacc cagcgcgccc agcgcgctgg cgaacgtcag caggccggag
     7081 tgcagcgtgg acaacccgta acccagctgc agcatcatcg gcagcagcag cgtgaagctg
     7141 cccacgccga tgcggaacag cgaaccgccg gccaccgacg cgcggaaggt ttccacgcgc
     7201 agcagcgaca ggcgcaggat cggccagcgc tcggcgcgcg cgtggcgcac gtacagcgcc
     7261 agcagcagcg cgccggcagc cagcacgccg gtggacacat cctgcggcag cacgcgcttg
     7321 ccgatgttct cgaagccgaa caccagccct gccagcccgc cgccgagcag ccaccagccg
     7381 gccaggtcga gcggcgcgtg cgcgctgtcg cgtgcgttgc cgacatggcg gcgcaccagc
     7441 tgccagccga gcacgccgat cggcaggttg atcaggaaga tccatggcca cgacgcgtag
     7501 gtgacgatca gcccgcccac cggcgggccg aggatgggcg cgaccagcgc ggggatggtg
     7561 acggtggcca tcgccgacac caggtcgctg cgcggcatct gccgcaccag caccagccgc
     7621 gccaccggca ccatcatggc gccgcccagg ccctgcagca cgcgcgcggc gaccagcgcg
     7681 ccgatgccgc gcgaggcggc gcagcccagc gagcccaggg tg
//
LOCUS       DASZZC010000471         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_360308,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000471 DASZZC010000000
VERSION     DASZZC010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360308"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VHE37_10025"
     CDS             <1..921
                     /locus_tag="VHE37_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT35913.1"
                     /translation="LGLAAFVDSRSLEETQYQPKISLNWQPDRQTSYFVHWTRGFKGG
                     GFNAFAYRDVDQELRFKPETTTEWGFDFKGSFFGGTLRPNLSLYLMNAKDFQVLGREK
                     PPVVGNSQTCVPIQGVSYCLPPSAVIGLGATRVFNAEHARAQGVEGDITWLAGGFSLF
                     ATLGYNDTKYILFTQNECPQTTTATTCDATGKSFAFAPRWDTTLTPSYILPLPWFGLS
                     LTPSFTAQYISRQFLDTHLSHEQPGFMRYNASLALGSIDHGWSLQIIGNNLTDVRTAV
                     RYGDVLPHFEISVPEAPRMVFAQLRWAFER"
     gene            935..2026
                     /locus_tag="VHE37_10030"
     CDS             935..2026
                     /locus_tag="VHE37_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVT35914.1"
                     /translation="MMRRHLRIWLTGAAALAAVACKHGGEAVDTGLGIGSFEKFHYAQ
                     DGVPARDYYVYLPTDLPGGPALLVYLHGCQQDATDAALGTRWNQEAQARKFIVVYPQQ
                     LRPSAEDAQGQLDGNGAGCWNWFEPQNWNRGAGEAATIAGITREVMAAHAVDPQRVYL
                     EGVSAGGMMASIMGATYADLYAAIGIDAGGGYPAGTDPTGALAAMAMNGYATRMPVIV
                     FAGTADEVDVYPLNVEAVQQWLATNDMLDDGTENGSVSPLPATLDNRGLDAAQLAHLG
                     PPGNLCVSPQHLASPCFGPAIGLQGSYPYTVEHYLDADGNPLIDFWTIYGLTHNYVGG
                     NPQGSFTDPLGPDITGAAWDFFLAHPRSP"
     gene            2023..>3499
                     /locus_tag="VHE37_10035"
     CDS             2023..>3499
                     /locus_tag="VHE37_10035"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVT35915.1"
                     /translation="MIQSTMMTLPLTLDRILERAGTMYPGVEIITRRPDRSLHRATYA
                     DMYRRARQLAQALQGAGLQPGDRVATLCWNHWVHLEAYFGIPAAGGVLHTLNLRLFPE
                     DIAYIAQHAQDRFLIVDDCLLPLYEKFSSVMKFERVIVVPFDGSIAHAHESYEDFIAA
                     PADDFQYPALDENAPCGMCYTSGTTGKPKGVVYSHRSSVLHALGMSLLDALGLSMRDT
                     VVPVVPMFHANAWGLPYACAMVGSRQVFPGPYLDAGSLLDLYSETGTTLTGGVPTIWM
                     SVLQALEREPARWNIRRGMRVLVGGSAVPPALIRSLDAYGIHIMAAWGLTETSPLASV
                     ARLRPELESAPQEAQLAARARAGLPLPLVELRIAGEQGMAPWDGRSVGEIEVRGPWVT
                     ASYHLRPDAADRFSADGWFRTGDVASMTPDGYIQISDRAKDLIKSGGEWISSLDLENE
                     LMAHPAVAEAAVIAVAHEKWMERPLAVVVRKPGAEVTPEQLR"
BASE COUNT          568 a         1202 c         1170 g          559 t
ORIGIN      
        1 ctcgggctgg cggcgttcgt cgacagccgt tcgctggagg agacgcagta ccagccgaag
       61 atctcgctga actggcagcc ggacaggcag accagctatt tcgtgcactg gacgcgcggc
      121 ttcaagggcg gcggcttcaa cgcgtttgcc taccgcgacg tcgatcagga actgcgcttc
      181 aagccggaga ccaccaccga gtggggcttc gacttcaagg gctcgttctt cggcggcacg
      241 ctgcggccca acctgtcgct gtacctgatg aacgcaaagg atttccaggt gctgggccgc
      301 gagaagccgc cggtggtggg caacagccag acctgcgtgc cgatccaggg cgtcagttac
      361 tgcctgccgc ccagcgcggt catcggcctg ggcgccacgc gcgtgttcaa cgccgagcat
      421 gcgcgtgcgc agggcgtgga aggcgatatc acctggctgg ccggcggctt ctcgctgttt
      481 gccacgctgg gctacaacga caccaagtac atcctgttca cgcagaacga gtgcccgcag
      541 accaccacgg ccactacctg cgacgcgacc ggcaagtcgt tcgccttcgc gccgcgctgg
      601 gacaccacgc tcacgcccag ctacatcctg ccgctgccgt ggttcgggct gagcctcacg
      661 ccgagtttca ccgcgcagta catcagccgc cagttcctcg acacgcacct gtcacacgaa
      721 cagccgggtt tcatgcgcta caacgcgagc cttgcgctcg gcagcatcga tcacggctgg
      781 agcctgcaga tcatcggcaa caatctcacc gacgtacgca ccgcggtgcg ttacggcgac
      841 gtgctgccgc acttcgagat cagcgtgccg gaagctccgc ggatggtgtt cgcgcaactg
      901 cgctgggcgt tcgagcgctg agcggcgtaa gcgcatgatg cggcgccatc tgcggatctg
      961 gctcaccggc gcagccgcgc tggccgccgt cgcctgcaag catggcggtg aagctgtcga
     1021 caccggcctg ggcatcggca gcttcgagaa attccattac gcgcaggatg gcgtgccggc
     1081 gcgcgactac tacgtgtacc tgcctaccga tctgcccggc ggcccggcgc tgctggtcta
     1141 cctgcacggc tgccagcagg acgcgaccga tgccgcgctc ggcacgcgct ggaaccagga
     1201 ggcgcaggcg cgcaagttca tcgtggtcta tccgcagcag ctgcgtccgt cggccgagga
     1261 cgcgcagggc cagctcgacg gcaacggcgc cggctgctgg aactggttcg aaccgcagaa
     1321 ctggaaccgc ggcgcgggcg aggcggcgac catcgccggc atcacgcgag aggtgatggc
     1381 cgcgcacgcc gtcgatccgc agcgcgtgta tctcgaaggc gtctctgccg gcggcatgat
     1441 ggccagcatc atgggcgcaa cctacgccga tctctacgcc gccatcggca tcgatgccgg
     1501 cggtggctac ccggccggca ccgatccaac cggcgcgctg gcggcgatgg ccatgaatgg
     1561 ctacgccacg cgcatgccgg tcatcgtgtt tgccggcacc gcggacgaag tcgacgtgta
     1621 cccgctcaac gtcgaggccg tgcagcagtg gctggccacc aacgacatgc tcgatgacgg
     1681 cacggagaac ggcagcgtgt cgccactgcc ggcgacgctg gacaaccgcg gcctcgacgc
     1741 cgcacagctg gcccatctcg gaccgccagg caatctctgc gtgtcgccgc agcacctggc
     1801 gtcgccctgt ttcggcccgg cgatcggcct gcagggcagc tacccgtaca ccgtcgagca
     1861 ttacctcgat gcggacggaa atccgctgat cgatttctgg accatctacg gcctcacgca
     1921 caattacgtc ggcggcaatc cgcagggctc gttcaccgat ccgctggggc cggacatcac
     1981 cggcgccgcc tgggatttct ttctcgcgca cccgaggtcg ccgtgatcca gtccaccatg
     2041 atgaccctgc cgctcacgct cgaccgcatc ctcgagcgcg cggggacgat gtatcccggc
     2101 gtcgagatca tcacgcggcg gccggaccgc agcctgcatc gcgccaccta cgcggacatg
     2161 taccggcgcg cgcgccagct ggcgcaggcg ctgcagggcg cgggcctgca gccgggcgac
     2221 cgcgtggcca cgctgtgctg gaaccactgg gtgcacctgg aagcgtactt cggcataccc
     2281 gcggccggcg gcgtgctgca cacgctcaac ctgcgcctgt tcccggaaga catcgcctac
     2341 atcgcgcagc acgcgcagga tcgtttcctc atcgtcgacg actgcctgct gccgctgtac
     2401 gagaagttca gcagcgtgat gaagttcgag cgcgtgatcg tggtgccgtt cgacggcagc
     2461 atcgcccatg cgcacgagag ctacgaggac ttcatcgccg cgccggcgga tgatttccag
     2521 taccccgcgc tcgacgagaa tgcgccttgc ggcatgtgct acacctcggg caccaccggc
     2581 aaacccaagg gcgtggtgta ctcgcaccgc tccagcgtgc tgcacgcgct gggcatgtcg
     2641 ctgctggatg cgctgggcct gtcgatgcgc gacaccgtgg tgccggtggt gccgatgttt
     2701 cacgccaacg cctggggcct gccgtacgca tgcgccatgg tcggcagccg gcaggtcttt
     2761 cctggcccgt atctcgacgc cgggagcctg ctcgatctct acagtgaaac cggcaccacg
     2821 ctcacgggtg gcgtgccgac catctggatg tcggtgctgc aggcgctcga gcgtgagccc
     2881 gcacgttgga acatcagacg cggcatgcgc gtgctggtcg gcggctccgc ggtgccgcca
     2941 gcgctgatcc gcagccttga tgcttacggc atccacatca tggccgcgtg gggtctcacc
     3001 gagacgtcac cgctggccag cgtggcgcgg ctgcgtcccg agctggaaag cgcgccgcag
     3061 gaggcgcagc tggccgcgcg tgcacgcgcc ggcttgccgc tgccgctggt cgaactgcgc
     3121 attgcgggag agcagggcat ggcgccgtgg gacgggcgca gcgtaggcga gatcgaggtc
     3181 cgtgggccgt gggtgaccgc gagctatcac ttgcggcccg acgcggcgga tcgcttttcc
     3241 gctgacggct ggttccgcac cggtgacgtt gcctcgatga cgcccgatgg ctacatccag
     3301 atcagtgacc gcgccaagga cctgatcaag tccggcggcg agtggatcag ctcgctggat
     3361 ctcgagaacg aactgatggc ccacccggcg gtggccgagg ccgcggtgat cgcagtggcg
     3421 cacgagaaat ggatggagcg cccgctggcc gtggtggtgc gcaagcccgg cgccgaggtc
     3481 acgcccgaac agctgcgtg
//
LOCUS       DASZZC010000472         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1064962,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000472 DASZZC010000000
VERSION     DASZZC010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064962"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VHE37_10040"
     CDS             <1..1002
                     /locus_tag="VHE37_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HVT35916.1"
                     /translation="LPGGVRVVFTVRERYYLLAYPRLSANLDGQTSYGGEVDWTNMFG
                     LNYGLEAKVSASDRREANRGRETDYTLGFSAPFIFDSRYSLSLELSHNLSPVQDTVSY
                     DETFDSAQAVVTRKLSGDGPASQGWMAGAGLLWQNENRAGPGVPQAYGESTAPLLQAS
                     YYDIRDRIFSAEGVAYGARIAGARRGLASEYNYSTLTLNYRRLMAVGQTEHQDINVYG
                     YAGVYFDGPPEVHNFALGGSSALRAYNANFIEGNAYYLLRGEYLRPLGWPWLRGALIL
                     EAGNTFENARAFTLNRVYTSLGAALRVRLPLFVNLQLEVGYAVPLQGGGGRFFGGRV"
     gene            1048..1314
                     /locus_tag="VHE37_10045"
     CDS             1048..1314
                     /locus_tag="VHE37_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HVT35917.1"
                     /translation="MADIKSAFEQAQKDSKTLTKRPGNDDMLFLYSHYKQASEGDVAG
                     SRPGMMDMIGRAKYDAWAKLKGTSKDDAMKKYVDKVAALLKTHK"
     gene            1375..>2095
                     /locus_tag="VHE37_10050"
     CDS             1375..>2095
                     /locus_tag="VHE37_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT35918.1"
                     /translation="MFHPSALLRAAAVAALALLGSCNNDSGSSGTVSGPVRHFFVIVL
                     ENKGYSTTFESSPAPSSYLAQTLPSMGVLLRQYYGTGHASLDNYITMISGQPPNALTQ
                     ADCPLFVNVTPGTLDPLEQNAIYIGQGCVFPASTQTIVDELEALGFSWKGYMQDLDDG
                     TTHTCRHPALNSQDTTENATAANQYATRHNPFMYFHSIIDQQARCDAHVVDLAVLNQD
                     LQSAATTPNYTFITPDLCADGH"
BASE COUNT          389 a          725 c          648 g          333 t
ORIGIN      
        1 ctgcccggcg gcgtgcgcgt ggtgttcacg gtgcgcgagc gctactacct gctggcgtac
       61 ccgcggctgt cggccaacct cgacggccag accagctacg gcggcgaagt cgactggacc
      121 aacatgttcg gcctgaatta cgggctggaa gcgaaggtga gtgcgtcgga ccgcagggaa
      181 gccaaccgcg gccgcgagac cgattacacg ctgggcttca gcgcgccgtt tatctttgac
      241 agccgctact cgctgagcct ggagctgagc cataacctca gcccggtgca ggatacggtc
      301 agctacgacg aaaccttcga cagcgcgcag gccgtggtca cgcgcaagct ctccggcgac
      361 ggccccgcca gccagggctg gatggccggc gcgggcctgt tgtggcagaa cgagaaccgt
      421 gccgggcccg gcgtgccgca ggcctacgga gaatccaccg cgccgctgct gcaggcgagc
      481 tactacgata tccgcgaccg catcttcagc gctgagggag tggcctacgg cgcgcgcatc
      541 gccggcgcgc ggcgcggact ggcatccgag tacaactaca gcacgctcac gctcaattac
      601 cgccggctga tggcggtggg ccagaccgag caccaggaca tcaacgtgta cggatatgcc
      661 ggcgtctact tcgacggtcc gcccgaggtg cataacttcg ccctcggcgg ttccagcgca
      721 ttgcgcgcct acaacgccaa tttcatcgag ggcaacgcct attacctgct gcgcggcgag
      781 tacctgcggc cgctgggctg gccctggctg cgcggcgcgc tgatcctgga ggccggcaac
      841 actttcgaga atgcgcgcgc gtttaccctc aaccgcgtgt acaccagcct gggcgcggcg
      901 ctgcgcgtgc gcctgccgct gttcgtcaat ctgcagctcg aagtgggtta cgccgtgccc
      961 ttgcagggcg gcggcggccg cttctttgga ggccgcgttt agcccgtgct ataaagcgcc
     1021 gcacgcagac ttcccgggga gggaaacatg gccgacatca agagcgcctt cgagcaggcg
     1081 cagaaagatt cgaagacgct gaccaagcgc ccgggcaacg acgacatgct gttcctctac
     1141 tcgcattaca agcaggccag cgaaggcgat gtcgccggca gccgcccggg catgatggac
     1201 atgatcggcc gcgccaagta cgacgcctgg gccaagctca agggcacgag caaggacgac
     1261 gccatgaaga aatacgtcga caaggttgcc gcactgctga agacccacaa gtaagacctg
     1321 cactgcggcg gcacctgcgc tgcgggcgac attcagttcc accggggttg cgtcatgttc
     1381 catccttccg ccttgcttcg tgccgctgcc gtggcagccc ttgcgctgct gggctcctgc
     1441 aacaacgaca gcggctcgtc cggcacggtc agcggcccgg tgcggcattt cttcgtcatc
     1501 gtgctcgaga acaagggcta ctcgaccacc tttgaatcca gcccggcgcc gtcgtcctac
     1561 ctggcgcaga ccctgccgtc gatgggcgtg ctgctgcgcc agtactacgg caccggccac
     1621 gccagcctgg acaactacat caccatgatc agcggccagc cgcccaacgc gctgacccag
     1681 gcggactgcc cgctgttcgt caatgtcacg cccggcacgc tcgacccgct ggagcagaac
     1741 gccatctata tcggccaggg ttgcgtgttt cccgcgtcca cgcagaccat tgtcgacgaa
     1801 ctcgaagcac tgggcttcag ctggaagggc tacatgcagg acctggatga cggcaccacg
     1861 cacacctgcc gccatcccgc gctcaacagc caggacacca cggagaatgc caccgcggcc
     1921 aaccagtacg ccacgcgcca caatccgttc atgtacttcc acagcatcat cgaccagcag
     1981 gcgcgctgcg atgcgcacgt ggtcgacctg gcggtgctca accaggatct gcagagcgcc
     2041 gccaccacgc ccaactacac tttcatcacg ccggacctgt gtgccgatgg ccacg
//
LOCUS       DASZZC010000473         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_554349,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000473 DASZZC010000000
VERSION     DASZZC010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554349"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VHE37_10055"
     CDS             complement(<1..529)
                     /locus_tag="VHE37_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT35919.1"
                     /translation="MTTTESEVQARTRQRALIDFGQAPENRPRSVLVVDDDDFVRGSL
                     AKQLAFLGVARVVTAGDGDLACQALRVQGPFDVMISDLQMPGTDGIELLRRIHDIQPG
                     LSVLLISSMDGKVLGAAEELAGARGLRLLGCLPKPVQLSALRRTLERLDHGLTQPAAH
                     APQRSQVTSQELRDAI"
     gene            714..1673
                     /locus_tag="VHE37_10060"
     CDS             714..1673
                     /locus_tag="VHE37_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT35920.1"
                     /translation="MGNIVFFAFVGFAVVTAIMGFVAVPQGMEYTVERFGKYRLTLQP
                     GPHFIVPWLDRIGRKLSLMEQVLDVPSQEVITKDNAMVGVDGVVFYQVLDAPKAAYEV
                     QHLEIAILQLTMTNLRTVMGSMDLDELLSQRDHINTRLLSVVDQATTPWGVKVTRIEI
                     KDIRPPADLVESMGRQMKAERDKRAAILESEGLRQSAILKAEGARQAQILAAEGEKQQ
                     AILDAEGRKEAAFRDAEARERQAAAEAQAISVVSGAIAKGDVQAVNYFVATKYIEALG
                     RVATSPNEKLVLMPLEAGSVIGAIGGIAELAKKALAQNAAPKS"
     gene            1670..2104
                     /locus_tag="VHE37_10065"
     CDS             1670..2104
                     /locus_tag="VHE37_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HVT35921.1"
                     /translation="MIGEFLWWHWLLIGFVLLILEMVLPTEFVLLWMGVSAIMVGALS
                     GLVAVPWQLQCVLFGVLSMVSYFGYRKLRPRIDSDKPTLNRRGHSYVGRNFTLTVPIV
                     NGIGKLHVDDSQWRIAGPDLPAGSQVKVVDVDGTTLKVEASH"
     gene            2171..2854
                     /locus_tag="VHE37_10070"
     CDS             2171..2854
                     /locus_tag="VHE37_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HVT35922.1"
                     /translation="MVPIESLRGLNEDLYSLGEEIAHAVTHGLGAVLAIAGLCVLVAR
                     AALYGNQWHVISAAIFGSTLVMMYTASTLYHSIPLPRARHVLRVIDHSMIYLLIAGSY
                     TPFTLVALHGPWGWTLFAIVWTLAAAGVAFKIWNTGRFENLSLGIYLGMGWLGIAAIG
                     PLLKAVPAGGLWMMLIGGITYSGGVLFYTWERLRYHHAIWHLFVLGGSVLHYFAVLFY
                     VLPGPTAPR"
     gene            2948..>3229
                     /locus_tag="VHE37_10075"
     CDS             2948..>3229
                     /locus_tag="VHE37_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35923.1"
                     /translation="MNARLIERAAAECLAAPGVRFRAARAATRLPGGAPDKKQRVELL
                     AQELKQLDALQRKLYAGKSRALLVIFQAMDAAGKDSTIRAVFTGVDPAGC"
BASE COUNT          554 a         1052 c         1069 g          554 t
ORIGIN      
        1 ctatcgcatc gcgcagttcc tgcgacgtca cctgcgagcg ctgcggcgca tgcgccgccg
       61 gctgcgtcag gccgtggtcg agtcgttcca gcgtccggcg cagcgccgac agctgcaccg
      121 gcttgggcag gcagccgagc aggcgcagtc cgcgcgcacc agccagttcc tcggcggcgc
      181 cgagcacctt gccgtccatg ctgctgatca gcagcaccga caggccgggc tggatgtcat
      241 ggatccggcg cagcagttcg atgccgtcgg tgcccggcat ctgcaggtcg gaaatcatca
      301 cgtcgaatgg gccctgcacg cgcagcgcct ggcaggccag gtcgccatcg ccggcggtga
      361 ccacgcgggc cacgcccagg aacgccaatt gcttggccag cgagccgcgc acgaaatcgt
      421 cgtcatcgac caccagcacc gagcgcggcc ggttttccgg cgcctggcca aaatcaatga
      481 gtgcgcgctg acgtgtgcgg gcctggacct cggattctgt cgttgtcatg agaacttccg
      541 gcactcgggc ggccgccatg cggcccttcg catgcttgaa cggcagagtt aagcaatcct
      601 gaatagacaa atgttcgcgc agcggccgcg cggcgtgatg gcagcatcag ccgtcaagga
      661 ctgccgctac aatcgggcca ctaccgggct gaccgtcaac ggggagactt tcgatgggca
      721 acatcgtctt ttttgcgttt gtcggctttg ccgtggtcac cgccatcatg ggcttcgtgg
      781 cagtgccgca gggcatggaa tacacggtgg agcgcttcgg caagtaccgc ctgacgctgc
      841 agccggggcc gcacttcatc gtgccctggc tcgaccgcat cgggcgcaag ctgtcgctga
      901 tggagcaggt gctggacgtg ccctcgcagg aagtcatcac caaggacaac gccatggtcg
      961 gggtcgacgg tgtcgttttc tatcaggtac tcgacgcgcc caaggccgcg tacgaagtgc
     1021 agcacctgga gatcgccatc ctgcagctga ccatgaccaa cctgcgcacg gttatgggct
     1081 cgatggatct cgatgagctg ctctcccagc gcgatcacat caatacgcgc ctgctgtcgg
     1141 tggtcgatca ggccaccacg ccgtggggcg tgaaggtgac gcgcatcgag atcaaggaca
     1201 tccgcccgcc ggcggacctg gtcgaatcga tgggccggca gatgaaggcc gagcgcgaca
     1261 agcgcgccgc cattctcgaa tccgaaggcc tgcgccagtc ggcgatcctc aaagccgaag
     1321 gcgcgcgcca ggcgcagatt cttgcagccg aaggcgagaa gcagcaggcc attctcgacg
     1381 ccgaaggtcg caaggaagcc gcgttccgcg atgccgaagc gcgcgagcgc caggccgcgg
     1441 cggaagcgca ggcgatcagc gtggtcagcg gcgccatcgc caagggcgac gtgcaggcgg
     1501 tgaattattt cgtcgccacc aagtacatcg aggcgctggg ccgtgtcgcc accagcccga
     1561 acgaaaaact ggtgctgatg ccgctggaag ccggcagcgt gatcggcgcc atcggcggca
     1621 tcgccgagct ggccaagaaa gcgctggcac agaacgcggc gcccaaatcg tgatcggcga
     1681 attcctctgg tggcactggc tgctgatcgg cttcgtcctg ctgatcctgg aaatggtgct
     1741 gcccaccgag ttcgtgctgc tgtggatggg cgtgtcggcg atcatggtcg gcgcgctgtc
     1801 cggcctggtc gcggtgccgt ggcaactgca gtgcgtgctg ttcggcgtgc tgtcgatggt
     1861 cagctacttc ggctaccgaa agcttcggcc gcgcatcgac agcgacaagc ccacgctcaa
     1921 ccgccgcggg cattcctacg tcggccgcaa cttcacgctc accgtgccca tcgtcaacgg
     1981 catcggcaaa ttgcacgtgg acgattcgca gtggcgcatc gcagggccgg acctgccggc
     2041 cggctcgcag gtaaaggtgg tcgacgtcga tggcaccacg ctgaaggtgg aggcatccca
     2101 ttaagcgccc gggtctgcgc gcgaaactct atcgcgcgcg caagctgcgc cagcgcgcac
     2161 gccgctgccg gtggtgccga tcgagagcct gcgcggcctc aacgaggacc tctacagcct
     2221 gggcgaggag attgcgcacg cggttacgca cgggctcggc gcggtgctgg ccattgccgg
     2281 cctgtgcgtg ctggtggcgc gcgccgcgct gtacggcaac cagtggcacg tgatcagcgc
     2341 ggcgattttt ggcagcaccc tggtgatgat gtacaccgcc tccacgctgt accacagcat
     2401 cccgctgccg cgcgcgcgcc acgtgctgcg cgtgatcgac cactcgatga tctacctgct
     2461 gatcgccggc agctatacgc cgttcacgct ggtggcgctg cacgggccgt ggggctggac
     2521 cctgttcgcc atcgtgtgga cgctggccgc ggctggggtc gccttcaaga tctggaacac
     2581 cggccgtttc gaaaacctgt cgctgggcat ctacctgggc atgggctggc tgggtatcgc
     2641 cgccatcggt ccgctgctca aggcggtgcc tgcgggcgga ctgtggatga tgctgatcgg
     2701 cggcatcacc tactccggtg gcgtgctgtt ctacacctgg gagcggctgc gctaccacca
     2761 cgccatctgg cacctgttcg tgctcggcgg cagcgtgttg cactacttcg cggtattgtt
     2821 ttacgtgctg ccgggcccca ccgcaccccg ctgagcgatg ttataaaaaa ttgtctgttt
     2881 ttaaaacaaa tagataatta ctaacatcgg aaacggcctt gcggtggagt ccaagaacag
     2941 gacccgcatg aacgccaggc tgatcgaacg tgccgctgca gagtgtctcg ccgcacccgg
     3001 cgtgcgcttc cgcgccgcgc gcgcggccac ccgcctgccc ggcggcgcac cggacaagaa
     3061 gcagcgcgtc gaactgctgg cgcaggagct caagcagctg gatgcactgc agcgcaagct
     3121 ctacgccggc aaaagccgcg cgctgctggt gatcttccag gccatggatg cggcgggcaa
     3181 ggacagcacc atccgcgcgg tgttcaccgg cgtcgatccg gccggttgc
//
LOCUS       DASZZC010000474         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_490269,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000474 DASZZC010000000
VERSION     DASZZC010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490269"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="VHE37_10080"
     CDS             complement(<1..1691)
                     /locus_tag="VHE37_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HVT35924.1"
                     /translation="MKRILINATQPEELRVAIVDGQKLQDLDIELASREQKKGNIYKG
                     RITRVEQSLEACFVEYGAERHGFLPLKEVARGYFRVNGGDRLEEGQEVIVQVEKEERG
                     TKGAALTTYISLAGRYLVLMPNNPRAGGVSRRAEGDEREEAKELLAKLNMPDGMGLII
                     RSNGIGRTAEELQWDLDHRIAYWNAIVEEADKRPSPFLIYQENNIVVRALRDYLRPDI
                     GEVIVDNEDVYNAAREQMSYSMPADLPKLKLYRDEAPLFQRFQVESQIELAHERHVRL
                     PSGGAIVIDHTEALTSIDINSAKATGGRDIEETAFNTNLEAADEVARQLRLRDLGGLI
                     VIDFIDMNSQKNQREVEKRIEQACEVDRARIQFGRLSRFGLLEMSRQRLRPSLGEHTQ
                     IPCPRCNGRGQIRSVESLSLSILRLIEEESMKDRTGRVIAQVPVDVGTFLLNEKRLAV
                     REIEARCRVSVTLVPNPTMHSPHFEIKRVRADHLQQDNNSATSYALAQNFDAQAHEEM
                     AQPAAPAARPMEAAVKAIVPATPAPIVIQQAAAPAPVAAAPVMQPVPTLGIWQRFL"
     gene            2013..>2971
                     /locus_tag="VHE37_10085"
     CDS             2013..>2971
                     /locus_tag="VHE37_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVT35925.1"
                     /translation="MKQRDTRRPEGGKSEGRSGRTEGRRSRNHGQRPEGRKPKGRPAP
                     FAKPARRDGKVRKSDAPRNQVVASAGAAGVHMEVRKVEVGAREDGQRIDNFLLRQLPG
                     VPKSHVYRLLRSGQVRINGGRAKPERKLASGDEVRIPPVRMAEKGAAVRPPDAVLNRL
                     REAIVYEDANYLALNKPAGFASHGGTGIPFGVIEAARAWGKHEFLELAHRLDRDTSGL
                     LLLCKTRPALLRAQRAFKEGLAQKRYFALLCGRWSGGERDVDAALVRNRLASGERYVE
                     IDEEDGKPSHSRFTPQARYRDATLCEVEIFSGRTHQIRVHALAL"
BASE COUNT          454 a          959 c         1039 g          519 t
ORIGIN      
        1 aggaagcgct gccagatgcc cagcgtgggc accggctgca tcaccggcgc tgcggcaaca
       61 ggagcaggcg ccgccgcctg ctggatgaca atgggcgccg gcgtggcggg cacgatggcc
      121 ttgaccgcag cctccatcgg ccgggcggcg ggcgccgccg gctgcgccat ctcttcgtgc
      181 gcctgcgcat cgaagttctg cgccagcgca taactcgttg cggaattgtt gtcctgctgc
      241 aggtgatcgg cacgcacgcg cttgatctcg aaatgcggcg agtgcatggt gggattgggc
      301 accagcgtca ccgacacgcg gcagcgcgcc tcgatctcgc gcacggccag gcgcttctcg
      361 ttgagcagga aggtgccgac gtcgaccggc acctgcgcga tgacgcgccc ggtgcggtcc
      421 ttcatgctct cttcctcgat caggcgcagg atggacagcg acagcgattc caccgagcgg
      481 atctggccgc ggccgttgca gcgcgggcag ggaatctgcg tgtgctcgcc cagcgaaggg
      541 cgcaggcgct ggcgcgacat ctccagcagg ccgaagcgcg acaggcggcc gaactggatg
      601 cgcgcgcggt ccacctcgca ggcctgctcg atgcgcttct cgacttcgcg ctggttcttc
      661 tgcgagttca tgtcgatgaa gtcgatcacg atcagcccgc ccaggtcgcg caggcgcagc
      721 tggcgcgcca cttcgtcggc cgcctcgagg ttggtgttga acgcggtttc ctcgatgtcg
      781 cgtccgccgg ttgccttggc cgagttgatg tcgattgagg tcagcgcctc ggtgtggtcg
      841 atgacgatgg cgccgcccga cggcaggcgc acgtggcgct cgtgcgccag ttcgatctgc
      901 gattccacct ggaagcgctg gaacagcggc gcctcgtcac ggtacagctt gagcttgggc
      961 aggtcggccg gcatcgagta gctcatctgc tcgcgcgcgg cgttgtacac gtcttcgttg
     1021 tcgacgatca cttcgccgat gtccgggcgc aggtagtcgc gcagtgcgcg caccacgatg
     1081 ttgttttcct ggtagatcag gaacgggctg gggcgcttgt cggcctcttc cacgatcgcg
     1141 ttccagtagg cgatgcggtg gtcgaggtcc cactgcagtt cctcggcggt gcggccgatg
     1201 ccgttgctgc ggatgatcag gcccatgccg tcgggcatgt tgagcttggc cagcagctcc
     1261 ttggcctcct cgcgctcgtc gccctcggca cggcgcgaga cgccgccggc gcgcgggttg
     1321 ttgggcatga ggaccaggta gcggccggcc agcgagatat aggtggtgag cgccgcaccc
     1381 ttggtgccgc gttcttcctt ctccacctgc acgatgactt cctggccttc ttccaggcgg
     1441 tcgccgccgt tgacgcggaa gtagccgcgg gcgacttcct tcagcggcag gaagccgtgg
     1501 cgctcggcgc cgtattcgac gaagcaggct tcgaggctct gctcgacgcg ggtgatgcgg
     1561 cctttgtaga tgttgccttt cttctgctcg cggctggcga gctcgatatc aagatcctgc
     1621 agcttctggc cgtcgacgat cgccacgcgc agctcttccg gctgcgtggc attgatcaaa
     1681 atgcgtttca ttgttgtggt ctcgtgatgc gcgcgccggc aaggcgggag accgagtggt
     1741 gaccggcatg gtgctcgtcg tcatgagtcg ctcgtcttgc ggtgcgctgc gtgtgtcgtg
     1801 ggtagctgct gcgcccggaa aggacgcggc agccgcgatt cgagtaggaa gggggctgct
     1861 acaatctgcg gcaggcccgt tccgacctgt cttccgttaa ccgcctcagc gacaacggaa
     1921 tttctccgcc aaaaaacgga gtgtcatatt ttggcacaga tcggcggcgt gcaacagttt
     1981 ttatccacta cctgtccaca agcggtcatt ccatgaagca gcgtgacacc cggcgccccg
     2041 agggcggtaa gagcgagggc cgcagcgggc ggacagaagg gcgcaggtcc aggaatcacg
     2101 gacagcggcc ggagggacgc aagccgaaag gccgtcccgc gccatttgcc aagccggcgc
     2161 gccgcgacgg caaggtgcgc aagtccgacg cgccgcgcaa ccaggtggtc gccagcgcgg
     2221 gcgccgcagg cgtacatatg gaagtacgca aggtcgaagt gggcgcgcgc gaggacggcc
     2281 agcgcatcga caacttcctc ctgcgccagt tgcccggcgt gccgaagtcg cacgtctatc
     2341 gcttgctgcg ctccggccag gtgcgcatca acggcgggcg cgcgaagccc gagcgcaagc
     2401 tggcgtccgg cgacgaggtg cgcatcccgc cggtgcgcat ggcggagaag ggcgcggcgg
     2461 tgcgcccgcc cgacgccgtg ctcaaccgcc tgcgcgaggc gatcgtctac gaagacgcca
     2521 actatctcgc gctgaacaag ccggcgggat ttgcctcgca tggcggcacc ggcattccgt
     2581 tcggcgtgat cgaggcggcg cgtgcctggg gcaagcacga gttcctggaa ctggcgcacc
     2641 ggctcgaccg cgacaccagc gggcttctgc tgttgtgcaa gacgcgtcca gcgctgctgc
     2701 gcgcgcagcg cgcgttcaag gagggcctgg cccagaaacg ctacttcgcg ctgctgtgcg
     2761 ggcgctggag cggtggcgag cgcgacgtcg acgccgcgct ggtgcgcaat cgcctggcaa
     2821 gcggcgagcg ctacgtcgag atcgacgagg aagacggcaa gccctcgcac tcgcgtttca
     2881 cgccgcaggc gcgctaccgc gacgccacgc tgtgcgaggt ggaaatcttc tccggacgca
     2941 cgcaccagat ccgcgtgcac gcgctggcgc t
//
LOCUS       DASZZC010000475         5784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1193030,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000475 DASZZC010000000
VERSION     DASZZC010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193030"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VHE37_10090"
     CDS             <1..1733
                     /locus_tag="VHE37_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HVT35926.1"
                     /translation="AREPNHPFLQVPEPEPEDAFGGEQFRHTVPMLSIAKSYTQKEVD
                     AFINRVATAAAELGIPRADVRIAVTPKIDGNSIMDYGDKLVTRGKNGTGYVVTSLLDK
                     GVVVIGGERGGPCELAVSLKYFDEVLSGDFDHPRNMVAGLAGSDDLSEAHKQALEAGA
                     IHLVPYRTLDARELTLDELSANWATVADEVQGVDYLCDGAVASVSSERLRSYMGMTSD
                     SHRWNLAIKRNDEFGTTTVIAVDVTPGRTGKLVPRLQLAPLRLSGCTITYVTAHTASR
                     VTDMKLGPGAVLQIVRSGGVIPKLHKLETPSTVEVDFSKCPSCGTATETRGAHLFCPN
                     SLTCGPQAIRNLGHFFKTISVCKGFGPSVVEKLTAAGKATVRQVYDMSVEDFVAAGIS
                     AGVAKNLVAELTRSRQVPIEDAIFLGAHGCPDLGRGDSRKVLRHVPIAQLRTLTVERL
                     QGIRGFGEKTSASIVRELGKRLDLIESMLALGFNLEATPLEGSQAAGGSPIAGKTVVF
                     TGTMTSGSRDDMEAEARKLGASVGGSVTGKTNFLVCGEKVGAAKSDKALKLGVKVITE
                     AEYRAMLAGG"
     gene            1768..1965
                     /locus_tag="VHE37_10095"
     CDS             1768..1965
                     /locus_tag="VHE37_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35927.1"
                     /translation="MKSKTTLTAEHQARIDAAQKKIEEICVQADIPVMDLGHVLCKVA
                     RQVGGEDGIVGLAAAARWGGH"
     gene            1962..2378
                     /locus_tag="VHE37_10100"
     CDS             1962..2378
                     /locus_tag="VHE37_10100"
                     /inference="COORDINATES: protein motif:HMM:NF037906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35928.1"
                     /translation="MTAALYRYGVPNRPIGFATVPPGYVEVQPRPADGVYRDHARHGI
                     VVYDRPLAERDVYGFELIPLLDAEQIEAAAGACAKALAEYRDDLLEMETTEPHFVRAT
                     ILSHPGRAERVTCSVGEPDAFVKRVMALLREQVATA"
     gene            2462..3877
                     /locus_tag="VHE37_10105"
     CDS             2462..3877
                     /locus_tag="VHE37_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HVT35929.1"
                     /translation="MTLDSSLELDLAGGDAEIARTIAVETLHRATSIYTRSAIVEQLL
                     KDAGWPGAGGLLCDPSAGDGSFLDTALKCFLEVRPTATPDQVLAAVEGWEIHGGACRE
                     ARERLAGILSAHGWDVGTAAVTAAGMVRHGDFLTEGPRTPRFSVCAGNPPYLRAENVP
                     GVLREAYDLVVPDHAKSDMLHAFLDRCADVVLPDGVVCMVTSDRWLSNSGAARLREAL
                     GRRLKIKHLSRIDGQSAFYRPKTRRAGTPPRVHPVTVILERSDVPAVAELDGSPIYPG
                     AMVEESPLGTRTLADVATVRLSPWLGTNGIFVVGPEVARTLPEEFLTPVADGRDFTGG
                     LFKGPRKFAIRTEGVELPGVIIDHLKREMPRMCARGRRDPWTLPPENWWGKIDLSRPS
                     LVVPRICKGLTPIHIPAGVLCINHAVTVHQAAAMSLDQVDAVLRRAESQRWIEQRAAR
                     LENGYVSVTTTLLRRLPVPEA"
     gene            3953..4435
                     /locus_tag="VHE37_10110"
     CDS             3953..4435
                     /locus_tag="VHE37_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35930.1"
                     /translation="MQGLAKIDLNREKELRMNAVTKLGLGLCLAGLAAVWAWGCAGHD
                     QAPAVVVNVEQLFSAATVYKAEHGDMPGGPTGASPLVLADLVDAKLVDPATLTGEFNA
                     VQANEILHSARPGYYSLNVLGNDLIVHATLGDPETARRAAEKLGGSAADGVITVRRKI
                     "
     gene            4621..4800
                     /locus_tag="VHE37_10115"
     CDS             4621..4800
                     /locus_tag="VHE37_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35931.1"
                     /translation="MKTKTLLIALMMAATVAGSIPSTASASDAVTSSVSTASFSDWLA
                     GLFVSTDIKKSTKPG"
     gene            4904..5635
                     /locus_tag="VHE37_10120"
     CDS             4904..5635
                     /locus_tag="VHE37_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35932.1"
                     /translation="MEFRIDETVVLAQTGAKGRIVARVRADDDPRKCVPRGFPPLRMR
                     GDIAQRDAVTYLVLPEGERELKWSKRNPIEPFIPAQSTQRGARDADVAAQARGISDGT
                     MASLTGYRTTDEIQRVRALFVQHIIASGAAQYRSWIHAWRWFLNSEVGDRIPLLSRQE
                     RLYVYAMPGQAHHAGDIALGETEYVPHDATAFTLDEREFQSAAARENGYLVRVGYVCR
                     YALVGLASSRFMETVMRLRDERRAA"
BASE COUNT         1013 a         1709 c         1993 g         1069 t
ORIGIN      
        1 aggcgcgcga gcccaatcac ccgttccttc aggttcccga gccggaaccg gaggatgcgt
       61 tcggcggcga gcagttccgt cacacggtcc cgatgctgtc gatcgccaag agctacacgc
      121 agaaggaagt cgatgcgttc atcaatcgcg tggccactgc ggcggcggag ctgggtatcc
      181 cgcgcgctga cgtgcgcatc gccgtcacgc ccaagatcga cggcaacagc atcatggatt
      241 acggcgacaa gctggtgacg cgcggcaaga atggcactgg ctatgtggtg acctccctgc
      301 tcgacaaggg cgtggtcgtc atcggcggcg agcgcggtgg cccctgcgaa ctggcggtgt
      361 cgctgaagta tttcgatgaa gtgctctcgg gcgacttcga tcacccgcgc aacatggtgg
      421 ccgggctggc tggctcggac gacctgtcgg aagcgcacaa gcaggcgttg gaagctggcg
      481 ctattcatct cgtcccctat cgcacgctcg atgcgcgcga actgacgctg gatgaactgt
      541 cggccaactg ggcaacggtt gccgacgaag tgcagggcgt ggattacctc tgcgacggcg
      601 cggtggcgtc ggtttcttcc gagcgtttgc gctcgtacat ggggatgacc tccgattcgc
      661 acaggtggaa cctggcgatt aaacggaatg acgaattcgg cacgacgacg gtgatcgccg
      721 tggacgtgac gccggggcgc accggcaagc tggtgccgcg cctgcaactg gctccgctgc
      781 gcctgtcggg ttgcaccatc acttacgtga cggcgcacac cgccagccgc gtgacggaca
      841 tgaaacttgg ccccggcgct gtccttcaaa tcgtgcgcag cggcggcgtg attccgaaac
      901 tgcacaagct cgaaacgccc tctacggtag aggtggattt cagcaagtgc ccgtcctgcg
      961 gtacggccac ggagacacgc ggtgcgcacc tgttctgccc caactcgctg acctgcgggc
     1021 cgcaagcgat ccgcaacctg ggtcatttct tcaagacaat ttcggtgtgc aagggcttcg
     1081 gccccagcgt ggtggagaaa ctgaccgctg ctggcaaggc caccgtgcgt caggtgtacg
     1141 acatgagcgt cgaggacttc gtggccgctg gcatctccgc tggcgtcgcc aagaatctcg
     1201 tcgcggaact gacgcgcagc cgtcaggtgc cgattgaaga tgccatcttc ttgggcgcac
     1261 atggttgccc ggacctgggg cgcggagatt cgcgcaaggt acttcgccat gtccccatcg
     1321 cgcagttgcg cacgctgacc gtcgagcgcc ttcagggcat tcgcgggttc ggcgaaaaaa
     1381 ccagcgcctc catcgttcgt gagctgggca agcgcctgga cctcatcgag tcgatgctgg
     1441 cgctgggctt caacctcgaa gcgacgccgc ttgaaggcag tcaggctgcg ggcggttcgc
     1501 ccatcgccgg aaagaccgtg gtgttcacgg ggaccatgac cagcggcagc cgcgacgaca
     1561 tggaagccga agcgcgcaag ctgggcgcga gcgtcggtgg ctcggtgacg ggtaaaacga
     1621 acttcctggt ctgtggcgag aaggtcggcg cggccaagag cgacaaggcg ttgaagctag
     1681 gtgtgaaggt catcacggag gctgagtacc gcgcgatgct cgctggcggc tgattgcgta
     1741 aggcgcgggt caggttaagt tggtgacatg aaatccaaga caaccttgac cgccgagcat
     1801 caggctcgga tcgatgccgc tcaaaagaag atcgaggaga tctgcgttca ggcagacata
     1861 cctgtgatgg acctgggcca cgtgctgtgc aaggttgctc ggcaagtagg tggcgaagac
     1921 ggcatcgtcg gccttgccgc ggccgcgcgc tggggcggcc attgacggcc gcgctctatc
     1981 gctacggcgt tccgaatagg cccatcgggt ttgcgacggt gccgccggga tacgtcgagg
     2041 tgcaacctcg acctgcggac ggggtctatc gtgaccatgc tcgccacggc atcgtggtgt
     2101 acgaccgccc gctggcggag cgggatgtct atggctttga gctgatcccg ttgctggatg
     2161 cggagcagat cgaggcggcg gccggtgcct gcgcgaaagc gctggccgaa taccgcgatg
     2221 acctgctgga gatggagacc acggaaccgc atttcgtgcg cgcgaccatc ttgtcccatc
     2281 cgggtcgggc ggagcgggtg acatgcagcg ttggtgagcc ggacgctttc gtgaagcgcg
     2341 tcatggcgct gctgcgcgag caggtggcga cggcgtgacc gccgaagaac taattgcggc
     2401 tggatactgc aagaccacgc gcggacgttg cgaatagtca cgcgtcgtct accgtggctg
     2461 catgacgctc gactcttcac tcgaattgga cctggctggt ggcgacgccg aaatcgcgcg
     2521 caccatcgct gttgaaactc tgcatcgtgc gaccagcatc tacacgcgct ctgcgattgt
     2581 ggagcagttg ctgaaggacg cagggtggcc tggcgccggt gggctgctgt gcgacccatc
     2641 cgctggcgat ggatcgttcc ttgatacggc gctcaaatgc tttcttgaag tgcggccgac
     2701 ggcgacgcca gatcaggtgc tggccgcggt tgagggctgg gagatccatg ggggcgcctg
     2761 ccgcgaggcg cgcgagcgct tggcaggcat tctgtctgcg cacggctggg acgtgggtac
     2821 tgcggcggtg accgccgccg ggatggtgcg ccacggggac tttctcacgg agggtccgcg
     2881 cacgccgcgc ttttccgtgt gcgccgggaa tccgccttat ctgagagctg aaaatgtgcc
     2941 tggcgtgttg cgagaggctt acgacctggt ggtgccggat cacgccaaga gcgacatgtt
     3001 gcacgccttc ttggatcgct gcgccgacgt ggtgctgcca gatggcgtgg tgtgcatggt
     3061 gacctccgac agatggctgt ccaactcggg cgccgcgagg ttgcgcgaag cgctgggccg
     3121 tcggctgaag atcaagcacc tgtcgcgtat cgacgggcag agtgcgttct accgtccgaa
     3181 gacgcggcgc gccggcacgc cgccgcgcgt gcacccagtg accgtcattc tggagcgttc
     3241 agacgttccg gccgtggccg agttggatgg atcgccgatc tatcccggcg cgatggtgga
     3301 agaatccccg ctgggcacgc gcacgctagc cgacgtggca acagtgcggc tgtcgccctg
     3361 gctgggcacc aacggcattt tcgtcgtcgg tcccgaggtg gcgcgcactc tgcctgaaga
     3421 atttctgacg ccggtggcgg acgggcgaga tttcaccggc ggcttgttca agggaccgcg
     3481 caagtttgcg atccgtactg agggcgtgga gttgcccggg gtcatcatcg atcatctcaa
     3541 gcgcgagatg ccgcgcatgt gcgcccgcgg ccggcgcgat ccgtggacgc tgccgccgga
     3601 gaattggtgg ggcaagatag atctgagccg gccgtccctg gtggtgccgc gcatctgcaa
     3661 gggccttact ccgatccata tcccggctgg cgtgctttgc ataaatcacg cggtgaccgt
     3721 gcaccaggca gcggccatgt cgctcgacca ggtcgacgct gtgctgcggc gcgccgagtc
     3781 ccagcgctgg atcgagcagc gtgccgcgcg tctggaaaac ggctacgtgt ccgtgacgac
     3841 caccttgctg cgccgcttgc cggtgccgga ggcttgatct gccaggctct gcatcggccc
     3901 gtacggtgcg aatcgggctg agtacgggca aacctgtacg caggaggttg cggtgcaagg
     3961 tttggctaaa atcgatttga acagagaaaa ggagttgaga atgaatgctg tgacgaagct
     4021 tggtttggga ttgtgtttgg caggtcttgc ggccgtatgg gcgtggggtt gcgctggaca
     4081 tgatcaagcg ccggcggtgg tcgtaaatgt tgaacagctg ttttcggctg caacggtcta
     4141 caaggcggag catggcgaca tgcccggcgg acccaccggc gcaagtccgt tggtgctcgc
     4201 tgacctggtg gatgcaaagc ttgtcgatcc agcgacgctg acgggtgagt tcaatgcggt
     4261 gcaggccaac gaaatcctgc acagcgcgcg gcccggctat tacagcctca acgtgctggg
     4321 caatgacctc atcgtccatg ccacgctggg cgatcctgaa accgcgaggc gcgcggcgga
     4381 aaagctgggc gggtcggcgg cggatggggt cattaccgtc aggcgaaaaa tctaaggacg
     4441 tcacccggcg gcgcacgagt gcctccaaag gctgactgtt caccgttttt ggcggttttc
     4501 tgctgcatcc ggttctaaag acggaattga ttccttcttt aggcggttcg cagtcaagga
     4561 gactggccgt agtttggggt cacctgctca acaacgtccc gcaaggacag gagacacccg
     4621 atgaaaacga aaaccctgct catcgccctg atgatggcgg ccaccgtcgc cggctcgatt
     4681 ccctccaccg cttcggcgtc cgacgcggtg accagctcag tgtccaccgc gtcgttcagc
     4741 gactggctcg ccggcctgtt cgtgtcgacc gacatcaaga agagcaccaa gccgggctaa
     4801 gggcaggtga gcctcgaata gggcctctcc ggaggcccta ttaatttgtg gggttgcatt
     4861 tttcacgatg gatttaggct gaagccaata catcggagag gacatggaat ttcggatcga
     4921 cgagactgta gtgctggcgc agacgggcgc gaagggcagg atcgtggcgc gggtgcgcgc
     4981 ggatgatgac cctcgcaagt gcgttcctcg cggattcccg ccgctgcgca tgcggggcga
     5041 cattgcccag cgcgacgccg taacctacct ggtgctcccc gaaggcgagc gcgagttgaa
     5101 gtggtcgaag cgaaacccca tcgagccgtt tatccccgcg caatcgacgc agcgtggcgc
     5161 gcgcgatgcc gacgtggcgg cgcaggcgcg cggtatctcc gacggcacga tggcctcact
     5221 gacgggctat cggacgaccg atgaaatcca gcgcgtacgt gcgctgttcg tccagcacat
     5281 catcgccagt ggcgcggcgc agtaccgcag ctggatccac gcctggcgct ggttcctgaa
     5341 ctcggaggtg ggtgaccgca tcccgctgct gtcgcggcag gagcggctgt acgtctacgc
     5401 catgccgggg caggcccacc atgcgggcga cattgcgctg ggagagaccg agtacgtccc
     5461 tcacgacgcg acggccttca cgctcgacga gcgcgaattc cagagcgccg cagcgcgcga
     5521 aaatgggtac ctggtgcgcg tgggctatgt gtgccgttac gcgctggttg ggctggcttc
     5581 ctcacgattc atggaaacgg tgatgcgcct gcgcgacgag cggagggctg cttgagttcg
     5641 gtcccggcca gtgatttgcg gcgcgctttg gcgaagctgt cggacgcggt gctgatgcac
     5701 ctgcatttca tgcgcctggc attacagccg ccggtaggcg ccggcaccgc gggcgtgctc
     5761 gagcgcctga tgtcggggct ggag
//
LOCUS       DASZZC010000476         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_360732,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000476 DASZZC010000000
VERSION     DASZZC010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360732"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1306)
                     /locus_tag="VHE37_10125"
     CDS             complement(164..1306)
                     /locus_tag="VHE37_10125"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HVT35933.1"
                     /translation="MDEKQLHAVLTNPPLHWALAAGIAAGIYLLALLFKRVLLRRLSA
                     LAGHTSTKIDDVALMVLQSTQLWLVLVVAIEAGTQMLDLPGKLDRLCAQAATVAVFLQ
                     VGFWAGALAEYFIARNRERALATNAGAATSLAALNFIVRIALWALVVLLALDNLGINI
                     TALVAGLGIGGVAVALAVQNILGDLFASLSIVIDKPFVIGDFIICDTYMGTVEYVGLK
                     TTRLRSLDGEQIIFSNGDLLKARVRNYKRMFERRVLWGFDVVYQTTPEQLQKIPALMR
                     ELIEAQKKVRFERAHFAALKDTGLHFESVYWVLDPDYNLYMDIQQALYLELLRRLSAM
                     GVTLAYSSVTARPDAPNDQGAALGRAQDRADDAGGDAGDVADAARH"
     gene            1421..>1952
                     /locus_tag="VHE37_10130"
     CDS             1421..>1952
                     /locus_tag="VHE37_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HVT35934.1"
                     /translation="MAKNLANKRVAILATDGFEQSELLEPRKALIEAGARAEVVSPKQ
                     GRIKAWKDKDWGEEVAVDKALSTADANDYDALVLPGGVMNPDHLRMEPKAVSFVRAFF
                     EANKPVAAICHGPWTLVEAGVVQGRKLTSWPSLKTDITNAGGEWVDQEVVTDQGLVTS
                     RKPGDIPAFNRKMIEEF"
BASE COUNT          360 a          642 c          659 g          291 t
ORIGIN      
        1 ctggcgacgt tattaattat tgatcttatt tttaataaat caataaaact taacatcgaa
       61 aatgcaggtg cggtggaggc agaaaatcgg cgcggggcgg atcgggcggt catggggcgg
      121 cgtcagcaag cgcgcattat ccgcgccgcc gcgcaccggc gcatcagtgg cgcgcggcgt
      181 ccgccacgtc gccggcgtcg ccgccggcat cgtcggcgcg atcctgcgcc cggcccagcg
      241 ccgcgccctg gtcgttcggc gcgtcggggc gcgccgtcac cgacgagtag gccagcgtca
      301 cgcccatggc ggacagccgc cgcagcagct ccaggtagag ggcctgctgg atgtccatgt
      361 acaggttgta gtcggggtcg agcacccagt acacgctctc gaaatgcagc ccggtgtcct
      421 tcagcgcagc gaagtgcgcg cgctcgaagc gcaccttctt ctgcgcctcg atcaactcgc
      481 gcatcagcgc ggggattttc tgcagctgct ccggcgtggt ctggtacacc acgtcgaaac
      541 cccacagcac gcggcgctcg aacatgcgct tgtagttgcg cacgcgcgcc ttcagcaggt
      601 cgccgttgga gaagatgatc tgctcgccgt cgagcgagcg cagccgcgtg gtcttcagcc
      661 ccacgtactc caccgtgccc atgtaggtgt cgcagatgat gaagtcgccg atgacgaagg
      721 gcttgtcgat gacgatggac agcgaggcga acaggtcgcc caggatgttc tgcaccgcca
      781 gcgccaccgc aacgccgccg atgcccaggc ccgcgaccag cgcggtgatg ttgatgccca
      841 ggttgtccag cgccagcagc accaccagcg cccacagcgc gatgcgcacg atgaagttga
      901 gcgcggccag gctggtggcg gcgccggcat tggtggccag cgcgcgctcg cggttgcggg
      961 cgatgaaata ttccgccagc gcgccggccc agaaacccac ctgcaggaac accgcgacgg
     1021 tggcagcctg cgcgcacagc ctgtccagct tgcccggcag gtcgagcatc tgggtgccgg
     1081 cctcgatggc gacgaccagc accagccaca gctgggtgga ctgcagcacc atcagcgcca
     1141 cgtcgtcgat cttcgttgac gtgtgcccgg ccagcgccga cagccggcgc agcagcacgc
     1201 gcttgaacag cagcgccagc aggtagatgc cggcagcaat gccggcggcc agcgcccagt
     1261 ggagcggcgg gttggtcagc accgcgtgca attgtttctc gtccacggtt tctccttgca
     1321 aaaacgtcag acccggcgcg gcggcgccgg gttcgaactc gcgcgccggc tgcgggtcgt
     1381 agacaacagg aatgcatcac cggcaaagga ggctggcctc atggcaaaga acctggcgaa
     1441 caagcgcgtc gccatcctgg cgaccgatgg cttcgagcaa tcggaactgc tggaaccgcg
     1501 caaggcgctg atcgaggcgg gcgcccgcgc cgaagtggtg tcgcccaagc agggccgcat
     1561 caaggcgtgg aaggacaagg actggggcga ggaagtggcg gtggacaagg cgctgtcgac
     1621 cgccgacgcc aacgactacg acgcgctggt cctgcccggc ggcgtgatga accccgacca
     1681 cctgcgcatg gaacccaaag ccgtgtcgtt cgtgcgcgca ttcttcgagg cgaacaagcc
     1741 ggtggcggcg atctgccacg gcccgtggac gctggtcgag gccggcgtgg tgcaggggcg
     1801 caagctcacc tcgtggccct cgctgaagac cgacatcacc aatgccggcg gcgaatgggt
     1861 ggaccaggaa gtggtcaccg accagggcct ggtcaccagc cgcaagcccg gcgacatccc
     1921 cgcgttcaac cgcaagatga tcgaggagtt cg
//
LOCUS       DASZZC010000477         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_168129,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000477 DASZZC010000000
VERSION     DASZZC010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168129"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VHE37_10135"
     CDS             <1..950
                     /locus_tag="VHE37_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT35935.1"
                     /translation="YGGLGLGAVELALVQEQAGRRLLCAPYFSSVCLAANALRHCATA
                     AAREKYLPLLAQGALRATMPLGRADAAQAVRTTQGWKLESDALHVPDGADAQLLLVPA
                     RIDDQLTALFALEPGTPGMSITPLQSWDATRRFARVELRGVALPQQARIDEAPRAAVG
                     WMSALALARLCIAAEALGGAQACLDLAVNYTGTRKQFGRAIASFQAVKHRCAEMRVKV
                     EALRSAVYGAAALAASGAPVEDMAQECAMAKALSADTFFWCAQEAIQLHGGVGFTAEY
                     DPQLYFKRAQASSHWLGDATALRERIARRLFEAAPHPHS"
     gene            1047..>1894
                     /locus_tag="VHE37_10140"
     CDS             1047..>1894
                     /locus_tag="VHE37_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT35936.1"
                     /translation="MSDAAFRDEIAAWMQAHLTGRFAPLKHRGGPGDEEAFPELRKEW
                     ERELASGGWTCVGWPKECGGRGLSIAQQVIYHEEYARAGGPGRMGHIGEGLLGAALIR
                     FGSEAQKKRFLPEIVAGREYWAQGYSEPNAGSDLANVQTRAEKQSDGSWKVTGQKIWT
                     SNAQDSDWIFVLARAEAGSRGNKGLVFLLMPLKQKGIDIRPIHQMAGGAEFNEVFFDG
                     AIAQAEHLVGAVGDGWKVAMGLLEAERGVSTLGQQMHFAHELDLVIEAARANGKSRDP
                     HIRQRIA"
BASE COUNT          315 a          618 c          686 g          275 t
ORIGIN      
        1 agtacggcgg cctcggcctg ggcgcggtgg aactggcgct ggtgcaggag caggccgggc
       61 ggcgcctgct gtgcgcgccg tatttctcca gcgtgtgcct ggcggccaac gcgctgcggc
      121 attgcgcgac ggccgccgcc cgcgagaaat acctgcccct gctggcgcag ggcgcgctgc
      181 gcgcaaccat gccgctgggc cgcgccgatg ccgcgcaggc agtcaggacc acccagggct
      241 ggaagctgga gagcgatgcg ctgcacgtgc ccgacggcgc cgatgcgcag ctgctgctgg
      301 tgcccgcgcg catcgacgat cagctcaccg cgctgttcgc gctcgagccg ggcacgccgg
      361 gcatgagcat cacgccgctg cagagctggg acgccacgcg ccgcttcgcg cgcgtggaac
      421 tgcgcggcgt tgcgctgccg cagcaggcgc gtatcgacga ggcaccgcgc gcggcggtcg
      481 gctggatgag tgcgctggcg ctggcgcgcc tgtgcatcgc ggccgaggcg ctcggcggcg
      541 cgcaggcctg cctcgacctg gcggtgaact acaccggcac gcgcaagcag ttcggccgcg
      601 ccatcgcctc gttccaggcg gtgaagcatc gctgcgccga gatgagggtg aaggtggaag
      661 cgctgcgctc cgcggtgtat ggcgccgccg cgctggccgc cagcggcgcg ccggtggaag
      721 acatggcgca ggaatgcgcg atggccaagg cgctgtcggc cgacaccttc ttctggtgcg
      781 cacaggaagc gatccagctg cacggcggcg tcggcttcac cgccgagtac gatccgcagc
      841 tgtacttcaa gcgcgcgcag gcctcgtcgc actggctggg tgacgccacc gcgctgcgcg
      901 aacgcatcgc gcggcggttg tttgaagctg caccgcaccc gcattcctga tgttataaat
      961 tattcaatga aaaataattc atataataat ttagaacatg ccacaggccg gtgcggtggg
     1021 atgtttctgg tctgcggagc gcacccatga gcgacgccgc cttccgcgac gagatcgccg
     1081 cgtggatgca ggcgcatctc accgggcgat ttgcgccact gaaacatcgc ggcggcccgg
     1141 gcgatgaaga agcctttccc gagctgcgca aggaatggga gcgcgagctg gcgtccggcg
     1201 gctggacctg cgtgggctgg ccgaaggaat gcggcgggcg cgggctgtcc atcgcgcagc
     1261 aggtgatcta ccacgaggaa tacgcgcgcg ccggcgggcc gggccgcatg ggccacatcg
     1321 gcgaaggact gctgggtgcc gcgctgatcc gcttcggctc tgaagcgcag aagaaaagat
     1381 tcctgccgga gatcgtcgcc ggccgcgagt actgggcgca gggttattcc gagcccaacg
     1441 ccggttccga tctcgccaac gtgcagacgc gcgcggaaaa gcagagcgac ggttcgtgga
     1501 aagtcaccgg ccagaaaatc tggaccagca acgcgcagga cagcgactgg atcttcgtgc
     1561 tggcgcgcgc cgaggccggc agccgcggca acaagggcct ggtattcctg ctgatgccgc
     1621 tgaagcagaa gggcatcgac atccgcccga tccaccagat ggccggcggc gccgagttca
     1681 acgaggtgtt cttcgacggc gccatcgccc aggccgagca cctggtgggc gcggtcggcg
     1741 acggctggaa ggtggcgatg ggcctgctcg aagccgagcg cggcgtgtcg acgctgggcc
     1801 agcagatgca cttcgcccat gagctggacc tggtgatcga agcggcgcgc gccaatggaa
     1861 aatcgcgcga cccgcacatc cgccagcgca tcgc
//
LOCUS       DASZZC010000478         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_294578,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000478 DASZZC010000000
VERSION     DASZZC010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294578"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VHE37_10145"
     CDS             complement(<1..1533)
                     /locus_tag="VHE37_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HVT35937.1"
                     /translation="MTGRRLRAAVFFCALLAQAARAGASADASYLAELQARAHATVLA
                     QQPAWLRLGHWRPDRIGHGYNSTIDSPGFFLAKDGRGEPAAELDATLELFFSGTQIAG
                     EPAQCRYLARYQWLRRQLEFDPARLPEASCERYQEWARGLDAARIAVVFASNDLNSPS
                     SMFGHTLLRIDTHGQGDNERLLAYALNYAAADVNDGGVTYAWRGITGGYEGYFSIYPY
                     YDKVKEYARFEHRDLWEYPLRLAADQVQLLLWHVWELRGVGSSYYFFSENCSYQLLSL
                     IEVARPDLDLTGPFRRGVAYTIPVDTIRALRDSGLLDAPAYRPAAAKVFQHDYHQLGD
                     DEQAWVQRYVRGEAGFDDAVFARADDAGRARMLEMAHEQLYFLFQQGEMPRSAGLERD
                     HAALVERSRLPVDSGFAPAPQPAMPDQGHSSARTAAGARIDAHAAAAVLRWRPSYHDR
                     LDPPAGYLSGGEIRFFDVSALAGDGGVRLDQVTLLSVQAVSPRDATFKPWSWQVALGA
                     RRL"
     gene            complement(1548..2024)
                     /locus_tag="VHE37_10150"
     CDS             complement(1548..2024)
                     /locus_tag="VHE37_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HVT35938.1"
                     /translation="MMRKTLTLAVLALAPLSAFADHDAGCGLGTQLWAGQQGLVAKVL
                     AATTNASFGNQTFGMTSGTLGCSKDGVVTAANRLPMFASANLDQLSADMASGHGEALA
                     ALASLYGVDDADRVAFYRTTQQHYAAIFTSSGVTAAQVLAGVDATLRADGRLAHYV"
     gene            complement(2111..>2702)
                     /locus_tag="VHE37_10155"
     CDS             complement(2111..>2702)
                     /locus_tag="VHE37_10155"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35939.1"
                     /translation="ERYKLFVAGRYEAYAAKPHFEASAPAATAIGTQEPGLLLYFIAG
                     KQPGAQVENPRQVSAFNYPKEHGPRSPTFSRSTLLRPSDADGGALLLVSGTASIVGHA
                     SKHPGDAPAQLEEILGNLSALLNAAEAELADRDHAWRVDVMKLYLRHASHRLLLEERL
                     RTAFGKDLALITLQGEICRSELLAEVEAVFSRVPRF"
BASE COUNT          431 a          910 c          959 g          402 t
ORIGIN      
        1 gaggcgtcgc gcgcccagcg caacctgcca tgaccagggt ttgaacgtgg cgtcgcgcgg
       61 cgatacggcc tgcactgaga gcagggttac ctggtcgagg cgcacgccgc catcgcccgc
      121 cagcgcggag acatcgaaga agcggatctc gccgccgctg aggtaaccgg ccggcgggtc
      181 gaggcggtcg tgatacgacg gccgccagcg cagcacggcc gctgccgcgt gcgcatcgat
      241 gcgcgcccct gccgccgtgc gcgcgctgga atggccctgg tccggcatcg cgggttgcgg
      301 cgccggcgcg aagccggagt cgaccggcag ccggctgcgc tcgaccagcg ccgcgtgatc
      361 gcgctccagg cctgcgctgc gcggcatctc gccctgctgg aacaggaaat acagttgttc
      421 gtgcgccatc tccagcatgc gcgcacgccc ggcgtcatcc gccctcgcga aaaccgcatc
      481 gtcgaacccg gcttcaccgc gcacgtagcg ctgcacccag gcttgctcgt cgtcgccgag
      541 ctggtggtag tcgtgctgaa aaaccttggc cgcggccggc cggtaggcgg gggcgtcgag
      601 caggccggaa tcgcgcagcg cgcggatggt gtcgaccgga atggtgtagg ccacgccgcg
      661 ccggaatggg ccggtgaggt cgagatcagg ccgcgccacc tcgatcagcg acagcagctg
      721 gtaggaacag ttctcgctga agaagtagta ggacgagccg acgccccgca actcccacac
      781 atgccacagc agcagctgga cctggtcggc cgcgagccgc agcgggtact cccacaggtc
      841 gcggtgctcg aagcgcgcgt actccttcac cttgtcatag tacggataga tggagaaata
      901 accttcgtat ccgccggtga tgccgcgcca ggcgtaggtc acgcctccgt cgttcacgtc
      961 ggccgcagcg tagttgagcg cgtaggccag caggcgctcg ttgtcgccct gcccgtgcgt
     1021 gtcgatgcgc agcagcgtgt ggccgaacat cgaggacgga ctgttgaggt cgttcgaggc
     1081 gaacaccacc gcgatgcgcg cagcatccag gccgcgggcc cattcctggt agcgttcgca
     1141 ggacgcctcc ggcaggcgcg cggggtcgaa ttccagttgc cgacgcagcc actggtagcg
     1201 cgccaggtaa cggcattgcg cgggctcgcc ggcgatctga gtgccggaga agaacagttc
     1261 cagcgtggca tcgagctcgg ccgcgggttc gccgcgcccg tccttggcca ggaaaaatcc
     1321 gggggaatct atcgtgctgt tatagccgtg cccgatccgg tccggccgcc agtgcccgag
     1381 ccgcagccat gccggttgct gcgccagcac ggtggcgtgc gcccgcgcct gcaactccgc
     1441 caggtaggag gcgtcggcag aggctccggc gcgcgcggcc tgcgccagca gcgcgcaaaa
     1501 aaagacggcg gcacgcagcc gccgtccggt cacaggcaag atggcgatca gacgtagtgc
     1561 gcgaggcggc cgtcggcgcg cagcgtggca tcgacgccgg ccagcacctg tgccgcggtg
     1621 acgcccgacg aagtgaagat cgcggcgtaa tgctgctgcg tggtgcggta gaacgcgacg
     1681 cggtcggcat cgtcgacgcc gtacagcgaa gccagcgcgg caagcgcttc gccgtgaccg
     1741 ctggccatgt cggcagacag ctggtcgagg ttggcgctgg cgaacatcgg caggcggttg
     1801 gccgcggtaa ccacgccgtc cttggagcag cccagcgtgc cggacgtcat gccgaaggtc
     1861 tggttgccga aggatgcgtt ggtcgtcgcc gccagcacct tggccaccag gccctgctgt
     1921 ccggcccaca gctgcgtgcc caggccgcaa ccggcatcgt gatcggcgaa tgccgacagc
     1981 ggcgccagcg ccagcacagc gagagtcagc gtctttctca tcatgagtgc agtcctctgt
     2041 tcttgggatc ggacgatccc acgcggacca ccttagcgga tggtcaggcg ccacgccagc
     2101 gcggcgcagc ctagaaccgc ggcacgcggc tgaacaccgc ctccacttcc gccagcagtt
     2161 cgctgcggca aatctcgccc tgcagcgtga tcagcgccag gtccttgccg aaagcggtgc
     2221 gcagccgttc ttccagcagc aggcggtgcg aggcgtggcg caggtagagc ttcatcacgt
     2281 cgacgcgcca ggcgtggtcg cggtcggcca gttcggcctc ggcggcgttg agcagcgccg
     2341 acaggttgcc gaggatttcc tcgagctggg ccggcgcatc gccggggtgc ttgctggcat
     2401 ggccgacgat gctcgcggtg ccggatacca gcagcagcgc gccgccgtcg gcatcgctcg
     2461 ggcgcagcag cgtcgaacgc gagaaggtcg ggctgcgcgg cccgtgctcc ttcggatagt
     2521 tgaaggccga aacctggcgc ggattttcca cctgcgcgcc gggctgcttg ccggcgatga
     2581 agtacagcag cagtcccggc tcctgcgtgc cgatggcagt ggccgccggc gcgctggcct
     2641 cgaaatgcgg cttggcggcg taggcctcgt agcggccggc gacgaacagc ttgtagcgct
     2701 cg
//
LOCUS       DASZZC010000479         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_805967,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000479 DASZZC010000000
VERSION     DASZZC010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805967"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VHE37_10160"
     CDS             <1..509
                     /locus_tag="VHE37_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVT35940.1"
                     /translation="FPYLVDPNTGTALYESLDIIEYVARTYGGRVRIARGGRRAGAIG
                     AAVLASGMRGFRGYRARPSRAPEKPLELFSFESSPYSRLVREVLCELELPYLLHNTGK
                     GRRSDMGPPAFRDKWFKGPKDTTRNRRLLNQRTGRVQVPYLIDPNTGVEMYESRDIIA
                     YLEKTYGA"
     gene            499..1050
                     /locus_tag="VHE37_10165"
     CDS             499..1050
                     /locus_tag="VHE37_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT35941.1"
                     /translation="MAPETRAVTTWHLEQTDPAQLRRARVPEPAPMVMQAREPLPELN
                     RFLYSAVGGDWHWIDRLSWSHARWLEYLSRPQVQTWVLYVDGTPAGYVELEQQEQGNV
                     ELAYFGLIGKFIGRGLGGHLLTRGIEQAWAMPGTRRVWVHTCTLDGEHALANYQARGM
                     RLYHTDTSEKRVGATPGPWPGAR"
     gene            complement(1188..>1857)
                     /locus_tag="VHE37_10170"
     CDS             complement(1188..>1857)
                     /locus_tag="VHE37_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVT35942.1"
                     /translation="QRLHARGGEARTPTRGAGGVSALAPSGGWRAADARLPVPPRGGA
                     MALMSLFARVMGRKQVPDVFLVLHLNARLFWPWLVFASRLMPGGHLRARERELVILRT
                     AWNCRSRYEWGQHVEVGLRVGLADAEIVQLARAPQNFADERGRLLLAACDELFRDQAI
                     APPTWTALAAHYDAKRLIEIGMLAGHYQMLAGVLNSAGLALEPAIESVLQAFEQRAAA
                     IDIG"
BASE COUNT          326 a          652 c          619 g          260 t
ORIGIN      
        1 agttccccta cctggtcgat cccaacaccg gcaccgcgct gtacgagtcg ctcgacatca
       61 tcgagtacgt ggccaggacc tacggcggcc gcgtgcgcat cgcgcgcggc ggccggcgcg
      121 ccggggccat cggcgcggca gtgctggcca gcggcatgcg cggcttccgc ggctaccgcg
      181 cgcggccttc gcgcgcgccg gaaaaaccgc tggaactgtt ttccttcgag agcagcccgt
      241 actcgcggct ggtgcgcgag gtgctgtgcg aactggaact gccctacctg ctgcacaaca
      301 ccggcaaggg ccgccgcagc gacatgggcc cgccggcgtt ccgcgacaag tggttcaagg
      361 ggccgaagga caccacgcgc aaccgcaggc tgctcaacca gcgcaccggc cgcgtgcagg
      421 tgccgtacct gatcgacccc aacaccggcg tggagatgta cgagtcgcgc gacatcatcg
      481 cgtacctcga aaaaacctat ggcgcctgag acgcgcgcgg tcaccacctg gcacctggag
      541 cagaccgacc ccgcgcaact caggcgcgcg cgtgtgcccg aacccgcgcc gatggtcatg
      601 caggcgcgcg agccgctgcc ggaactgaac cgcttcctgt acagcgcggt gggcggcgac
      661 tggcactgga tcgatcgcct gtcgtggagc cacgcgcgct ggctggaata tctctcgcgc
      721 ccgcaggtgc agacctgggt gctgtatgtc gatggcacac cggccggcta cgtggaactc
      781 gagcagcagg aacagggcaa cgtcgaactc gcctacttcg gcctgatcgg caaattcatc
      841 ggccgcggcc tgggcgggca cctgctcacg cgcggcatcg agcaggcctg ggccatgccc
      901 ggcacgcggc gcgtgtgggt gcacacctgc acgctcgacg gcgagcacgc gctggccaat
      961 taccaggcgc gcggcatgcg gctgtaccac acggacacca gcgagaagcg agtcggcgca
     1021 acgccgggac catggccggg agcgcgatga agccgctgaa atgggccaac cgcaccccgc
     1081 attgacgtgt tattaattat cgttcaattt taaataaatt aaataattaa taacatcgga
     1141 aatggcggtg cggtggaggc aaaaatagtc tcgatgtgcc gccgccgtca tccaatgtcg
     1201 atcgcggcgg cgcgctgctc gaacgcctgc agcaccgact cgatcgccgg ctccagcgcc
     1261 aggcccgcgc tgttgagcac gcccgccagc atctggtagt ggccggccag catgccgatc
     1321 tcgatcagcc gcttcgcatc gtagtgcgcc gcgagcgcag tccacgtcgg cggcgcgatg
     1381 gcctggtcgc ggaacagttc gtcgcaggcg gcgagcagca ggcggccgcg ctcgtcggca
     1441 aagttttgcg gcgcgcgcgc cagctgcacg atctccgcgt cggccaggcc cacgcgcaaa
     1501 cccacctcga catgctggcc ccactcgtag cggctgcggc agttccacgc ggtgcgcagg
     1561 atcaccagct cgcgctcgcg cgcgcgcaaa tgtccgcccg gcatcaggcg cgaggcgaac
     1621 accagccacg gccagaacag gcgcgcgttg agatgcagca ccaggaacac gtccggcacc
     1681 tgcttgcgtc ccatgacgcg ggcgaacagc gacatcaggg ccatggcgcc tccgcgcggc
     1741 ggcaccggca ggcgtgcgtc ggccgcgcgc cagccgccgg aaggggcgag cgcgctcacg
     1801 ccgcctgccc cgcgcgttgg cgtgcgcgct tcaccaccgc gtgcatgtaa gcgttgg
//
LOCUS       DASZZC010000480        10620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_100481,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000480 DASZZC010000000
VERSION     DASZZC010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100481"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /gene="gspE"
                     /locus_tag="VHE37_10175"
     CDS             complement(<1..1408)
                     /gene="gspE"
                     /locus_tag="VHE37_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HVT35943.1"
                     /translation="MDIALRRPPFAFAKRHGVIVTEDRGDFVMAAARPGAAVDAVAET
                     RRFVGRPLRLHAVTPQEFDALLQKTYEGQTGAAAGLMEGLHDDAEDADLDALTQALAE
                     NQDLLESSDDAPVIKFINGLLIEAIKENASDIHIESFESRFTVRFRVDGVLREMMSPP
                     KALAPRLVSRIKVMAKLDIAEKRLPQDGRISRAFGGRKVDVRVSTIPTGQNHERVVMR
                     ILDKQAERLDLEHLGIGGSQIEVLKRVIHKPYGIILVTGPTGSGKTTTLYSSLAVLND
                     KSRNIMTVEDPIEYYIDGVGQTQVNTKVEMTFARGLRAILRQDPDVVMVGEIRDLETA
                     QIAVQASQTGHLVLSTLHTNTAVGAVTRLRDMGVEPYQLSSSLIGLMAQRLVRTLCKH
                     CKQPYDAARSELEHLGVPTNIQVQLFKPVGCPHCRHTGFLGRSGIHEIIEIDRKLEEM
                     IHDGSSEQALEAYARSKGP"
     gene            complement(1425..3614)
                     /gene="gspD"
                     /locus_tag="VHE37_10180"
     CDS             complement(1425..3614)
                     /gene="gspD"
                     /locus_tag="VHE37_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HVT35944.1"
                     /translation="MSLPSLRALRLLPAALALLVATGAGAATATLNLKDADINTLITM
                     VSEITGKNFIVDNRVKGKVTVISAKPMTPGGVYETFLGVLEVNGFAAVPDGQSIKIVP
                     EANARTDGGVFSGNGAGMPIDDLVTHVYTLENVSATQLVPILRQLMPQWAHLAAYQPS
                     NQLILADRAANVQRIERLIKQMDQASDRDIDLVKLQNASASEVVRTLTSLQQADKTTD
                     PSSRPGVILADERSNSVLVGGDKGERAKLLDIIARLDQPLKDDGATQVIYLHYASAEN
                     LAPILQGYAQQANSVKPGGSSGATAAAAAPVVSSGGGNSEVHVIADKDTNALIITASP
                     KVMRQVRDVIAQLDIRRSQVLVEAIIAEVSATKTSNLGVDWAVFNPDRVAMASVESSN
                     LSGLLSSAGSLASTTTTAVSSSSGIGAALGLLGQGATIGGGRISNNGQSGTSFAVLLH
                     ALQGDGDTNILSTPTLVTLDNQEAKFSAGQEVPFQTGSFTTNTTTGSATGLTGNPFST
                     FERKDVGLTLGVTPQINEGGTIKLKLDLEVSSVASGTIGTANVTTTKRKLTNTVGVDD
                     GQVLVIGGLIDDQYTDTTSGIPLLDRLPFIGGLFSSRAVNKIKRNLMVFIKPSILRKE
                     IDGDYYTRRKYEAVRQAQIDASVGSSSAVGGSRPLLENLEDYKAVAAPKPAVEPQVAP
                     APGAASPAPAPKPVPNADGPVLPPPDVPPLNPSEPEVPAPTQTPVPP"
     gene            complement(3611..4525)
                     /gene="gspC"
                     /locus_tag="VHE37_10185"
     CDS             complement(3611..4525)
                     /gene="gspC"
                     /locus_tag="VHE37_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HVT35945.1"
                     /translation="MISLPAPIAALAGKFNERHLRWLVAAVNGALAVAVGYALARLLW
                     TLLPIPAAMTWHPAPAAVAAPSAPAAAGTNLEAVVSAHLFGQYQAPTTQANLEAAPET
                     HLNLTLFGILAGSTDTDSRALIGQQNGDEAPYAVGQDVVHGVTLQAIFPDRVILSRNG
                     SLETLRLDKDAPIKGSAPAAVANNNSGDDDNDSSPGPANSGLAQIRETILSDPSKAAD
                     YIRVQPAVSPGGGTRGYRIYPGKDRTIFTSAGLRPGDLVTAVNGIQLDDGQKALQTLN
                     DLKGAGSVTLTLERGGQTQTVNVSLSNQ"
     gene            4763..6988
                     /locus_tag="VHE37_10190"
     CDS             4763..6988
                     /locus_tag="VHE37_10190"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT35946.1"
                     /translation="MKKNTLVRWALSAAVLLVFMLHVGGLVRFRLLDTIENFSYDARV
                     LATLPGTSDPRIVIVDLDEKTVAAEGWPWRRDKWARMIDQVFGVYHAKAAGFDIDFDA
                     PDDRAQRSFDDLMHTELANVPGLAERAAELREKLDYDRVFAAALKGKPVVLGMFFKRR
                     IEKGESAGIGGLCQPLLDKTAVGLYAVDFIQQQGYAGNQPVLTEAAPQCGFFDNESQD
                     DDGVLRRVPLMQMYDGAVYPSLALELVRLVQGNPAVELVFDPPDVRTSLHLEEVRVGE
                     ARAPVDGQVAVYVPYRGGYHTFQYVPATDVLNGKLEHPEVLKDAVVLFGASAAGLLDL
                     RTTPTSKGYAGVEVHANIVSGLLNGSIRQKAPYYNGIETVFLFLIAGMLAWGFIRLSP
                     AGSATLGLGIIAGIAALAFALWSGAHFIMPMGMPIAFTLSVMMVHLFYGFFIESRGKR
                     EISKLFGQYVPPELVEEMAATPEAISMEGESRDMTVLFSDVRNFTSISEKLDAKELAA
                     MMNAYLSKQTGVVQKHRGTIDKYIGDAIMAFWGAPLADKDHALNAVTSAMQMVSAVRE
                     LDADFEKRGWPKLSIGVGVNSGKMNVGNMGSSFRMAYTVMGDAVNLGARLEGLTKKYG
                     VSLLISDDTRNALPSDWAFREVDFVRVKGKNEPVGIYEPMGPKEQLDPGVRQDLARLR
                     GVMQLYRAQKWDQAEQEFFNLMQGERPLPLYEKYIERIMHWRENPPPKGWDGTWNWEE
                     K"
     gene            6985..8148
                     /locus_tag="VHE37_10195"
     CDS             6985..8148
                     /locus_tag="VHE37_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008002122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVT35947.1"
                     /translation="MSAFNWQDPLQVEEQLTSEERAVRDAAHAYAQGLLAPRVKQAFR
                     DESFDREVLREMGELGLLGLTLEGYGCPGGSHLMYGLAAREIERVDSGYRSSMSVQSS
                     LCMYPIYMYAREEVKRRLLPKLAAGELIGCFGLTEPDHGSDPGGMKTRAVRADGGWKV
                     SGTKNWITHAPVADVLVVWARADDIIRGFVLERGMAGLGTPKIEGKLSLRTSPTGQIM
                     MDEVFVPDAQVLQVQGLKGPFSCLHKARYGICWGAMGAAEACLLAARDYTLTRTQFGR
                     PLAANQLVQKKLADMQTEIALGTAAAMQLARLFDAGEATAEMVSLVKRNNCGKALEIA
                     RAARDMHGANGISDEYPVMRHLVNLETVNTYEGTHDIHALILGRALTGLAAFA"
     gene            8308..8625
                     /gene="clpS"
                     /locus_tag="VHE37_10200"
     CDS             8308..8625
                     /gene="clpS"
                     /locus_tag="VHE37_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HVT35948.1"
                     /translation="MSDTQREQNQGLAVEDAKPKVKPPPLYKVVMVNDDYTPMDFVVQ
                     TLQGFFHHSREKAVQIMLQVHTHGRGVAGVYPIEIAETKTAQVNSYARKHQHPLLTVM
                     EKE"
     gene            8629..>10620
                     /gene="clpA"
                     /locus_tag="VHE37_10205"
     CDS             8629..>10620
                     /gene="clpA"
                     /locus_tag="VHE37_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HVT35949.1"
                     /translation="MLSEELQKALDAAFVSARMDGHELLTVEHLLLALLSDTNVSEAL
                     TAAGADMEKLERALKDYIKNNVQSVKELERHEVQPTLSFQRVLQRAIYHVQAAGKKQV
                     TTLNVLVAMFAEKDTHAVYLLGEQGVTRLDVVNFISHGIAKNNSTAPQPAQENEEKDE
                     EGGKGPQGKTALEQYATNLNERAANGKIDPLIGRELEIERVMQTLCRRRKNNPLLVGE
                     AGVGKTAIAEGLAWLIVEGRVPDLLRNGVVYSLDLGALIAGTKYRGDFEKRFKAVMTE
                     LQKTQGAIVFIDEIHMIIGAGAASGGVMDASNLLKPLLAGGELRCIGSTTYQEYRGIF
                     EKDRALARRFQKIDVGEPSVEDAVKILEGLRERFEQHHQVQFTRGALRSAVELSTRHI
                     TDRHLPDKAIDVIDEAAARLRLLPENKKRKTVQVKDIEETVAKIARIPPKSVSSNDRD
                     KLATLERDLKLVIFGQDAAIEQLASSIKLSRSGLGSPDKPIGNFLLAGPTGVGKTEVT
                     KQLAQILGIELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAVIKHPHSVVLL
                     DEIEKAHPDVFNLLLQVMDHGTLTDNNGRKADFRNVILIMTTNAGAEESSRRSMGFAE
                     QSHSTDALEVIRKMFTPEFRNRLDAVVAFAPLGKSEIMRV"
BASE COUNT         1800 a         3346 c         3667 g         1807 t
ORIGIN      
        1 cggggccctt gctgcgcgcg taggcttcca gcgcctgctc cgagctgccg tcgtggatca
       61 tctcttccag cttgcggtcg atctcgatga tctcgtggat gccgctgcgg ccgaggaaac
      121 cggtgtggcg gcaatgcggg cagcctaccg gcttgaacag ctgcacctgg atgttggtcg
      181 gcacgcccag gtgttcgagc tcgctgcgcg cggcgtcata cggctgcttg cagtgcttgc
      241 acagcgtgcg caccaggcgc tgcgccatca ggccgatcag cgacgacgac agctggtacg
      301 gctccacgcc catgtcgcgc aggcgcgtga ccgcgccgac cgcggtgttg gtgtgcagcg
      361 tcgacagcac caggtggccg gtctgcgagg cctgcaccgc gatctgcgcg gtttccaggt
      421 cgcggatttc gccgaccatc accacgtcgg ggtcctggcg caggatggcg cgcaggccgc
      481 gtgcgaaagt catctcgacc ttggtgttga cctgcgtctg gccgacgccg tcgatgtagt
      541 actcgatcgg gtcttccacc gtcatgatgt tgcggctctt gtcgttgagc accgccagcg
      601 acgaatagag cgtggtggtc ttgcccgagc cggtggggcc ggtgaccagg atgatgccgt
      661 agggcttgtg gatgacgcgc ttgagcacct cgatctggct gccgccgatg cccaggtgct
      721 ccaggtccag ccgctcggcc tgcttgtcca ggatgcgcat gaccacgcgc tcgtggttct
      781 ggccggtggg gatggtcgac acgcgcacgt ccaccttgcg tccaccaaag gcacgcgaaa
      841 tgcgtccgtc ctgcggcagg cgcttctcgg cgatgtcgag cttggccatc accttgatgc
      901 gcgacaccag ccgcggcgcc agggctttgg gcggcgacat catttcgcgc agcacgccgt
      961 cgacgcggaa gcgcacggtg aagcggctct cgaacgattc gatgtggatg tccgaggcgt
     1021 tctccttgat cgcctcgatc agcaggccgt tgatgaactt gatcaccggc gcgtcgtcgg
     1081 acgattccag caggtcctgg ttttccgcca gagcctgggt cagcgcgtcg agatcggcat
     1141 cctcggcgtc gtcgtgcagg ccttccatga ggccggcggc ggcgccggtc tggccttcat
     1201 aggttttctg cagcagcgcg tcgaattcct gcggcgttac cgcgtgcagg cgcagggggc
     1261 ggccgacgaa gcggcgcgtt tcggcgacag cgtccacggc agcgccgggg cgcgcggcgg
     1321 ccatgacgaa atctccgcga tcctcggtga cgatgacgcc gtggcgcttg gcgaaagcga
     1381 acggcgggcg gcgcagcgcg atgtccatcg tgttgctggg cggatcaggg cgggaccggc
     1441 gtctgcgtgg gcgccggcac ttccggttcc gacgggttca gcggcggaac gtcaggtggc
     1501 ggaagcaccg ggccatctgc gttgggcacc ggcttcggtg cgggcgctgg cgaagcggca
     1561 ccgggtgccg gcgccacctg cggttcgact gcaggcttgg gcgccgcaac cgccttgtag
     1621 tcttccagat tctccagcag cggacggctg ccgccgacgg cggacgacga gccgacgctg
     1681 gcgtcgatct gtgcctggcg cactgcttcg tacttgcgcc gcgtgtagta gtcgccgtcg
     1741 atctccttgc gcaggatcga cggcttgatg aacaccatca ggttgcgctt gatcttgttg
     1801 accgcgcgcg acgagaacag cccgccgatg aagggcagcc ggtcgagcag ggggatgccg
     1861 ctggtggtgt cggtgtactg gtcgtcgatc aggccgccga tgaccagcac ctggccatcg
     1921 tccacgccca ccgtgttggt cagcttgcgc ttggtggtgg tgacgttggc cgtaccaatc
     1981 gtgccgctgg ccactgacga gacttccagg tcgagcttga gcttgatcgt cccgccctcg
     2041 ttgatctgcg gcgtcacgcc cagcgtgagg cccacgtcct tgcgttcgaa ggtggagaac
     2101 gggttgccgg tcaggccggt ggcgctgccc gtggtggtgt ttgtggtgaa cgagccggtc
     2161 tggaacggca cttcctggcc ggcggaaaac ttggcttcct ggttgtcgag cgtgaccagc
     2221 gtcggtgtcg acaggatgtt ggtgtcgcca tcgccctgca gtgcgtgcag cagtacggcg
     2281 aagctggtgc cgctctggcc gttgttgctg atgcggccac caccaatcgt ggcgccctgg
     2341 ccgagcaggc cgagcgccgc gccgatgccg ctgctggagc tgaccgccgt ggtcgtcgtg
     2401 ctggccaggc tgccggccga actgagcagg cccgacaggt tggagctttc cacgctggcc
     2461 atggccacgc ggtcggggtt gaacaccgcc cagtcgacgc ccaggttgct ggtcttggtg
     2521 gccgagactt cggcgatgat cgcctccacc agcacctgcg agcggcggat gtccagctgc
     2581 gcgatgacgt cgcgcacctg gcgcatcacc ttgggcgagg cggtgatgat cagcgcattg
     2641 gtgtccttgt cggcgatgac atgcacctcg ctgttgccgc cgccgctgct caccaccggt
     2701 gccgccgctg ccgcggtggc gccgctgctg ccgccaggct tcacgctgtt ggcctgctgc
     2761 gcgtagccct gcaggatcgg cgccaggttc tcggcgctgg cgtaatgcag gtagatgacc
     2821 tgggtggcgc cgtcgtcctt cagcggctgg tcgaggcgcg cgatgatgtc gagcagcttg
     2881 gcgcgctcgc ccttgtcgcc acccaccagc acgctgttgc tgcgctcgtc ggccaggatc
     2941 acgccgggcc gcgaggaagg atcggtggtc ttgtcggcct gctgcagcga ggtgagcgtg
     3001 cgcaccactt cgctggccga ggcgttctgc agcttgacca ggtcgatgtc gcggtcgctg
     3061 gcctggtcca tctgcttgat caggcgctcg atgcgctgca cgttggcggc gcggtcggcc
     3121 aggatcagct ggttgctcgg ctggtacgcg gccaggtgcg cccattgcgg catcagctgg
     3181 cgcaggatcg gcaccagctg cgtggccgag acgttctcca gcgtgtagac gtgcgtgacc
     3241 aggtcgtcga tcggcatgcc cgcgccgttg cccgagaaca cgccgccgtc ggtgcgcgcg
     3301 ttggcttccg gcacgatctt gatcgactgg ccgtccggca ccgcggcgaa gccgttcact
     3361 tccagcacgc cgaggaaggt ttcgtacacg ccgcccggcg tcatcggctt ggccgagatc
     3421 accgtgacct tgcccttcac gcggttgtcg acgatgaagt tcttgccggt gatctcgctc
     3481 accatggtga tgagcgtgtt gatgtcggcg tccttcaggt tcagcgtggc ggtggcggcg
     3541 ccggcaccgg tggcgaccag cagggccagc gcggcgggca gcaggcgcaa tgcccgtaga
     3601 gacggaaggc tcattgattg ctcagggata cgttcacggt ttgcgtctgg ccgccacgtt
     3661 cgagggtgag agtgacggaa ccggctcctt ttagatcgtt cagcgtctgc agcgctttct
     3721 ggccgtcgtc caattgtatg ccattgaccg cggtcacaag gtcgccaggg cgcagaccgg
     3781 cggaagtgaa aatggttcgg tccttgcccg gatagatgcg gtagccgcgg gtgccgccgc
     3841 cgggcgagac cgccggctgc acgcggatgt agtcggcggc cttcgacgga tcggacagga
     3901 tggtttcgcg gatctgcgcc agcccgctgt tggccgggcc cggagaggaa tcgttgtcat
     3961 catcgccgga attgttgttc gccaccgcgg ccggtgcgct gcccttgatc ggcgcgtcct
     4021 tgtccagccg cagcgtttcc agcgaaccgt tgcgcgacag gatcacgcgg tcggggaaga
     4081 tcgcctgcag cgtcacgccg tgcaccacgt cctggccgac ggcatagggc gcttcatcgc
     4141 cgttctgctg gccaatcagc gcacgcgagt cggtatcggt gctgcctgcg aggatgccaa
     4201 acagcgtgag gttcaggtgc gtttccggcg cggcttccag gttggcctgc gtggtcggcg
     4261 cctggtattg gccgaacagg tgcgcggaca cgaccgcttc caggttggtg ccggcagcgg
     4321 cgggcgcgga tggcgcggcg acagcggccg gcgctggatg ccaggtcatc gcggcgggta
     4381 tcggcagcag cgtccacagc aggcgggcca gcgcatagcc gacggccacg gccagcgcgc
     4441 cattgacggc agcgaccagc cagcggagat ggcgctcgtt gaattttccg gcgagcgcag
     4501 cgatgggagc ggggagcgag atcatgcgtg cgcgcgcatc ataaccgcgt gcgccatgca
     4561 cgggcatgac aaaacatcgg cgcggtgccc ggagggcatt ttttggccag caccgcaccg
     4621 cgccaggtga tgttattaaa aataatatat attttaatat aattaatatt ttgtaacatc
     4681 aaaaaacgcc gtgcggttgg cgtgctgcgg cacatttaag tactgtgcgg cggcacgaaa
     4741 agggaagccc ggagtcagcc ggatgaaaaa gaatacgctg gtgcgttggg cgctgagcgc
     4801 cgcggtgctg ctggtgttca tgctccacgt cggcggcctg gtgcggttcc ggctgctcga
     4861 taccatcgag aatttcagct atgacgcgcg cgtgctggcc acgctgccgg gcacctccga
     4921 cccgcgcatc gtcattgtcg acctcgatga aaaaaccgtg gccgccgaag gctggccttg
     4981 gcgccgcgac aagtgggcgc ggatgatcga ccaggtgttc ggcgtgtacc acgccaaggc
     5041 cgcgggcttc gacatcgact tcgacgcgcc cgatgaccgc gcgcagcgct cgttcgacga
     5101 cctgatgcac accgagctgg ccaacgtgcc ggggctggcc gagcgcgcgg cggagctgcg
     5161 cgagaagctc gactacgacc gcgtgttcgc cgccgcgctc aagggcaaac ccgttgtgct
     5221 tggaatgttc ttcaagcgcc gcatcgagaa aggcgagagc gcgggcatcg gcggcctgtg
     5281 ccagccgctg ctggacaaga ccgcggtcgg actctatgcg gtggatttca tccagcaaca
     5341 aggctacgcc ggcaaccagc cggtgctgac cgaggcggcg ccgcagtgcg ggttcttcga
     5401 caacgagagc caggacgacg acggcgtgct gcgccgcgtg ccgttgatgc agatgtacga
     5461 cggtgcggtg tacccgtcgc tggcgctgga actggtgcgc ctggtgcagg gcaacccggc
     5521 ggtggaactg gtgttcgatc cgcccgacgt gcgcacctcg ctgcacctgg aggaagtgcg
     5581 cgtgggcgag gcgcgcgcgc cggtcgacgg ccaggtggcg gtctacgtgc cgtaccgcgg
     5641 cggctaccac accttccagt acgtgcccgc caccgacgtg ctcaacggca agctcgaaca
     5701 tcccgaagtg ctcaaggacg ccgtcgtgct gttcggcgcc agcgccgccg gcctgctcga
     5761 cctgcgcacc acgcccacct ccaagggcta cgccggggtg gaggtgcacg ccaacatcgt
     5821 ctccggcctg ctcaacggca gcatccggca gaaggcgccg tactacaacg gcatcgaaac
     5881 ggtgttcctg ttcctgatcg ccggcatgct ggcctggggt ttcatccggc tgtcgccggc
     5941 cggctcggcc acgctggggc tcggtatcat cgccggcatc gccgcgctgg ccttcgcctt
     6001 gtggagcggc gcgcacttca tcatgcccat gggcatgccc atcgccttca ccctcagcgt
     6061 gatgatggtg cacctgttct acggcttctt catcgagtcg cgcggcaagc gcgagatctc
     6121 caagctgttc ggccagtacg tgccgccgga actggtggag gaaatggcgg ccacgccgga
     6181 agccatttcc atggaaggcg agagccgcga catgaccgtg ctgttctccg acgtgcgcaa
     6241 cttcaccagc atctcggaaa agctcgacgc caaggaactg gctgcgatga tgaacgccta
     6301 cctgtccaag cagaccggcg tggtgcagaa gcaccgcggc accatcgaca agtacatcgg
     6361 cgacgccatc atggcgttct ggggcgcgcc gctggccgac aaggaccacg cgctcaacgc
     6421 cgtcacctcg gccatgcaga tggtcagcgc ggtacgcgag ctggatgccg atttcgagaa
     6481 gcgcggctgg cccaagttgt ccatcggcgt gggcgtgaac agcggcaaga tgaacgtggg
     6541 caacatgggc tcctcgttcc gcatggccta caccgtgatg ggcgacgcag tgaacctggg
     6601 cgcgcgcctc gaaggcctga ccaagaaata cggcgtgagc ctgctgatca gcgatgacac
     6661 gcgcaacgcg ctgccgagcg actgggcctt ccgtgaagtc gacttcgtgc gcgtgaaagg
     6721 caagaacgag ccggtgggca tctacgaacc gatgggtccc aaggagcagc tcgaccccgg
     6781 cgtgcgccag gacctggcgc gcctgcgcgg cgtcatgcag ctgtaccgtg cgcagaagtg
     6841 ggaccaggcc gagcaggaat tcttcaacct gatgcaaggc gagcgcccgc tgccgctcta
     6901 cgagaaatac atcgagcgca tcatgcactg gcgcgagaat ccgccgccca agggctggga
     6961 tgggacgtgg aactgggaag agaaatgagt gccttcaact ggcaggatcc cctgcaggtc
     7021 gaggaacagc tcacatccga agagcgcgcg gtgcgcgacg ccgcgcacgc ctacgcgcag
     7081 ggcctgctgg ccccgcgggt gaagcaggcg ttccgcgacg agagctttga ccgtgaagtg
     7141 ctgcgcgaga tgggcgagct gggcctgctc gggctgacgc tggaaggcta cggctgcccg
     7201 ggcggctcgc acctcatgta cgggctggcg gcgcgcgaga tcgagcgggt cgactcgggc
     7261 taccgatcga gcatgtcggt gcagtcgagc ctgtgcatgt acccgatcta catgtatgcg
     7321 cgcgaggaag tgaagcgccg gctgctgccg aagctggccg ccggcgaact gatcggctgc
     7381 ttcggcctga ccgagccgga ccacggctcc gatcccggcg gcatgaagac gcgcgcggtg
     7441 cgcgccgacg gcggctggaa ggtttccggc accaagaact ggatcacgca cgcgccggtg
     7501 gccgatgtgc tggtggtgtg ggcgcgcgcc gatgacatca tccgcggctt cgtgctcgaa
     7561 cgtggcatgg ccgggctggg cacgccgaag atcgagggca agctgtcgct gcgcacctcg
     7621 cccacagggc agatcatgat ggacgaggtg ttcgtgcccg atgcgcaggt gctgcaggtg
     7681 caggggctca agggtccgtt ttcctgcctg cacaaggcgc gctacggcat ctgctggggt
     7741 gcgatgggtg cggcggaggc ctgcctgctg gcggcacgcg actacacgct gacgcgcacg
     7801 cagttcggcc ggccgctggc agcgaaccag ctggtgcaga agaagctggc cgacatgcag
     7861 accgagattg cgctgggcac tgccgcggcc atgcagctgg cgcgcctgtt tgatgccggc
     7921 gaagcgaccg cggagatggt gtcgctggtc aagcgcaaca actgcggcaa ggcgctggag
     7981 attgcgcggg ccgcgcgcga catgcacggc gccaacggca tcagcgacga atacccggtg
     8041 atgcgccatc tggtaaacct ggaaaccgtc aacacttatg agggcacgca cgacatccat
     8101 gcgctgatcc tcggccgcgc cctcaccggg ctggcggcat ttgcttgacg ctgcttttcg
     8161 tcagcccaaa aaaacctgct gtccggctgt gcttgaaaga cgtgaaaagc gtctcatatt
     8221 acaggtcgtg cagcgccttt cctgcgcgac tgttcggtaa gtttcacacg gtgtcgacag
     8281 ccggcgcagc ggagctaaac tagggtcatg tccgatacac agcgagaaca gaatcagggc
     8341 ctggcggttg aagatgccaa gcccaaggtc aagccgccgc cgctctacaa ggtggtgatg
     8401 gtgaacgacg actacacccc gatggatttc gtggtccaga ccttgcaagg ctttttccat
     8461 cactcgcggg agaaggcggt gcagatcatg ttgcaggtgc acacccacgg gcgcggcgtc
     8521 gccggcgtgt acccgatcga gatcgccgag accaagaccg cgcaggtcaa ttcctacgcg
     8581 cgcaagcacc agcatccttt gttgaccgtg atggaaaagg agtagttcat gctttccgag
     8641 gaactgcaaa aagcccttga tgccgccttc gtcagcgcgc gcatggatgg gcatgagctg
     8701 ctgacggtgg agcacctgct gctggcactg ctgtccgaca ccaacgtgtc ggaggcgctg
     8761 accgcggccg gcgccgacat ggaaaagctc gagcgcgcgc tcaaggacta catcaagaac
     8821 aacgtgcagt cggtgaagga actggagcgg cacgaggtgc agcccacgct gtccttccag
     8881 cgcgtgctgc agcgcgcgat ctaccacgtg caggccgccg gcaagaagca ggtgaccacg
     8941 ctcaacgtgc tggtcgccat gtttgccgag aaggacacgc acgcggtgta cctgctgggc
     9001 gagcagggcg tgacgcggct cgacgtggtc aatttcatca gtcacggcat cgccaagaac
     9061 aactccaccg cgccgcaacc ggcccaggag aacgaggaaa aggacgaaga gggcggcaag
     9121 ggcccgcagg gcaagaccgc gctggagcag tacgccacca acctcaacga gcgcgccgcc
     9181 aacggcaaga ttgatccgct gatcggccgc gagctcgaga tcgagcgcgt gatgcagacg
     9241 ctgtgccgcc ggcgcaagaa caatccgctg ctggtcggcg aggccggcgt gggcaagacc
     9301 gctatcgcgg aaggcctggc ctggctgatc gtcgagggca gggtgcccga cctgctgcgc
     9361 aacggcgtgg tctattcgct ggacctgggc gcgctgatcg ccggcaccaa gtaccgcggc
     9421 gatttcgaga agcgcttcaa ggcggtgatg accgagctgc agaagacgca gggcgccatc
     9481 gtgttcatcg acgagatcca catgatcatc ggcgccggcg cggcgtccgg cggcgtgatg
     9541 gacgcgtcca acctgctcaa gccgctgctg gccggcggcg agctgcgctg catcggcagc
     9601 accacctacc aggaataccg cggcatcttc gagaaggacc gcgccctcgc gcgccgcttc
     9661 cagaagatcg acgtgggcga gcccagcgtc gaggatgcgg tgaagatcct cgaaggcctg
     9721 cgcgagcgct tcgagcagca tcaccaggtg cagttcaccc gcggcgcgtt gcgcagcgcg
     9781 gttgagctgt ccacccgcca catcaccgac cgccacctgc cggacaaggc catcgacgtc
     9841 atcgacgagg cggcggcgcg cctgcgtctg ctgccggaga acaagaagcg caagacggtg
     9901 caggtcaagg acatcgagga aaccgtcgcc aagatcgcac gcatcccgcc caagagcgtg
     9961 tcgagcaacg accgcgacaa gctggccacg ctggagcgtg acctcaagct ggtgatcttc
    10021 ggccaggacg cagccatcga gcagctggca tcatcgatca agctgtcgcg ctcgggcctg
    10081 ggctcgccgg acaagccgat cggcaacttc ctgctggccg gccccaccgg tgtgggcaag
    10141 accgaggtga ccaagcagct ggcgcagatc ctgggcatcg agctgatccg cttcgacatg
    10201 agcgagtaca tggagcggca cacggtgagc cgcctgatcg gcgcgccgcc gggctatgtc
    10261 ggcttcgacc agggcggcct gctgaccgag gcggtgatca agcatccgca ttcggtggtg
    10321 ctgctcgacg agatcgagaa ggcccacccc gacgtgttca acctgctgct gcaggtgatg
    10381 gaccacggca cgctgaccga caacaacggg cgcaaggcgg atttccgcaa cgtgatcctg
    10441 atcatgacca ccaacgccgg cgccgaggaa agctcgcgcc gctcaatggg cttcgccgag
    10501 cagagccaca gcaccgatgc gctggaagtg atccgcaaga tgttcacgcc ggaattccgc
    10561 aaccgcctgg acgcggtggt ggcattcgcg ccgctgggca agagcgagat catgcgcgtg
//
LOCUS       DASZZC010000481         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_361198,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000481 DASZZC010000000
VERSION     DASZZC010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361198"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1570
                     /locus_tag="VHE37_10210"
     CDS             137..1570
                     /locus_tag="VHE37_10210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVT35950.1"
                     /translation="MDATAPAPGVGPMRRGWSSHWRIDSRTYRVMTALTWLLIILMTV
                     PDNLDYASLNTSYAPSEGSDLSRVLWIILLTGPLLIIAWRRTLASLLLRWINPFLLVF
                     MVMAIASLAWSDYPDVTARRLIRVLAVMLNALAFVLLGWHGRRFQHAVRPALTAMLIG
                     SIVFGLLRPDLAIHQETSPELLNAWRGLTNHKNTLGDAASLGFVLWLHAWLTRDGNRL
                     LSLCGCAAAGACLVLSRSSTAMMATIAVSAVMPALLWVPQSWRRWVKPGIVASLGLLV
                     TYSVVVLGLIPGLGVLLKPITHLTGKDMTFSGRTEIWAIIVKHIHLHPWLGTGYGGYW
                     IGPYPWAPVYEFLLKLHFYPGSAHNGYLEIVNDLGAVGLVALLGYLCLLLRDSLRLLS
                     FNNAQGVLLIGLFFQQAIANLSETHWWSVLSLDFTLMTLATTSLARALLDERLYRYFG
                     NPYAQAAETAAAERIEVTVHDVQPGFA"
     gene            1570..2682
                     /locus_tag="VHE37_10215"
     CDS             1570..2682
                     /locus_tag="VHE37_10215"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT35951.1"
                     /translation="MSPTAAPAGDIPSLNGIRALAVMLVFLAHAGLENLVPGGLGVTM
                     FFVLSGYLITTLMLIEYRQRGQVDLGAFYLRRMLRLMPPLALVLLTTWLLGAAGWIDG
                     SYTPAGFLSVLFYFGNYYVIAHDFNGVPAGIGVVWSLAVEEHYYLLYPWLALPLLQMR
                     RPQLSAAMLGALCVAVLGWRYWLATRGAPEAHLIMATDARLDAILYGCILALVANPWL
                     APPRQRSSRRDAVIAIACTLMLLGSLLERDPLFRVTLRYTLQCLAVSGLLYLAVARAA
                     DAPFRWLNLRPVVYVGTISYSIYLTHHVLLFAAARYLPATHPLIVAVIAALLTLTVAE
                     LMRRHVEEPCARLRRRLHHARTRPAPLQSSTQVTLQ"
     gene            2679..4157
                     /locus_tag="VHE37_10220"
     CDS             2679..4157
                     /locus_tag="VHE37_10220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVT35952.1"
                     /translation="MTAATALADLMPDAIPDHKARPNAADFERTPEQALEAAASQPGL
                     LLVDLDETLFLHNSTELFLECARPGILALLILRLLDGLRPWRYTGGIDTRDNWRVGVI
                     ALLFPWTWLVWRQRACRYAADHVNMPLRDALLRRSVAPVIVTAGFLPIVRTLCRAMEL
                     GDTRIIGCRLFALDDRRGGKLLLAKRTLGMQSICDATVITDSPSDLPVLHACSNRMLV
                     RWPQARFVPAFDRMYMPGQYIARVKHVGQRYILRGILQEDFAFWVLASIGLAAAPAWH
                     CTGLLLLLVSFWAIYERGYVDNDATGARHESDPKLSPEFHTSPVATPLLAPWIWATAS
                     GILGVLALWHPASPPPDAYLRWAVVLVAAHAWFWIYNRVDKGTRVWLYSGLQVGRTAA
                     FVALVPVSEIGVAALAAHVFARWTPYYFYRHGSNRWLTEQFHLTRLMMFLWLAVMLGL
                     ATGLDSVANGTGAMLLAWNLFRARHELRAAARHAHRIDREQP"
     gene            4154..>4581
                     /locus_tag="VHE37_10225"
     CDS             4154..>4581
                     /locus_tag="VHE37_10225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT35953.1"
                     /translation="MKAERVSICLATYRRPEQLAALLDDLLQQTRLPDEIVVIDNDSA
                     ASARAVVEALPAHAACPIRYQVQPEKNISITRNLTVAGASGNWLAFIDDDERAPRAWL
                     QQLLDARRRHDADGVLGPVLPVIPASAPGWIRRGRFYEWP"
BASE COUNT          669 a         1599 c         1421 g          892 t
ORIGIN      
        1 ctggcgacgg tcgccggcgg agttctggcg ctcagccggg cgcaacgcac ttcgctgagg
       61 gccacgcgcg aaatgctcag gcgcctggag gcaacgcgcg cggatctgct cggcgccgtc
      121 atcggccatt tctaggatgg acgcgacagc gcccgcgccc ggcgtcggcc cgatgcgccg
      181 gggctggagc agccactgga ggatcgacag ccgcacttat cgcgtgatga cggcgctcac
      241 ctggctgctc atcatcctga tgacggttcc cgacaacctc gactacgcca gtctcaacac
      301 gtcctacgcg cccagcgaag gcagcgactt gagtcgtgtg ctgtggatca tcctgctgac
      361 cggtcccctg ctgatcatcg cctggcgccg cacgctggcc tccctgctgc tgcgttggat
      421 caatccgttt ctgctggtgt tcatggtgat ggcgatcgcc agccttgcct ggtccgatta
      481 ccccgacgtg acggcgcggc ggctgatccg cgtactcgca gtgatgctca acgcgctggc
      541 attcgtgctg ctcggctggc acggtcgccg ctttcagcat gcggtgcgtc cggcgctcac
      601 ggccatgctg atcggctcaa tcgtcttcgg cctgttgcgt cccgatctcg ccatacacca
      661 ggaaacgtca cccgaactgc tgaatgcatg gcgcgggctt accaaccaca agaacaccct
      721 gggcgatgcg gccagcctcg gcttcgtgct gtggctgcat gcctggctga cccgcgacgg
      781 caaccggctg ctgtcgctgt gcggctgtgc cgcggccggt gcctgcctgg tgttgtcacg
      841 cagttccacg gcgatgatgg cgacgatcgc cgtctccgcg gtcatgccgg ctttgctgtg
      901 ggtgccgcaa agctggcggc gctgggtcaa gcccggcatc gttgccagtc tcggcctgct
      961 ggtgacctat tcagtggtgg tgctcggcct gattcccggc ctgggcgtgc tgctcaagcc
     1021 gatcactcat ctcaccggca aggacatgac cttcagcggc cgcaccgaaa tctgggccat
     1081 catcgtcaag catatccatc tgcacccgtg gctgggcacc ggctatggcg gctactggat
     1141 cgggccctat ccatgggccc cggtgtacga attcctgctc aagctgcact tctatccagg
     1201 ctcggcacac aacggctacc tggagatcgt caatgacctc ggcgctgtcg gactggtcgc
     1261 gctgctggga tacctgtgcc tgctgctgcg cgattccctg cggctgctgt ccttcaacaa
     1321 cgcgcaaggt gtactgctga tcggactgtt cttccagcag gccattgcca acctgtcgga
     1381 aacgcactgg tggagcgtgc tgtctctcga cttcacgctg atgacgctgg ccaccacttc
     1441 tcttgcccgg gccctgctcg atgagcgtct ataccggtac ttcggcaacc cctacgcgca
     1501 ggcggccgag accgctgccg ccgaacggat cgaagtcacc gtgcacgacg tgcagccggg
     1561 gtttgcctga tgagcccgac ggccgcgccg gcaggcgaca ttccttcgct caatggcata
     1621 cgtgcgctcg ccgtcatgct ggtgttcctg gcccacgccg gcctcgagaa cctcgtgccc
     1681 ggaggcctgg gcgtcaccat gttctttgta ttgagcggct acctcatcac cacgctgatg
     1741 ctgatcgaat accggcagcg cggacaggtc gacctggggg cattctactt gcgccgcatg
     1801 ctgcgcctga tgccgccact ggccctggtg ttgctgacca cctggctgct gggggccgcc
     1861 ggatggatcg acggcagcta cacgccggct ggcttcctgt cggtgctgtt ctacttcggc
     1921 aactactacg tcatcgcgca tgatttcaat ggcgtgcccg ccggcatagg cgtggtctgg
     1981 tcgctggcag tggaagagca ttactacctg ctttatccct ggctggcgct acccctgctg
     2041 cagatgcggc ggccgcagct cagtgccgca atgctcggcg cgttgtgcgt ggccgtgctc
     2101 ggctggcgct actggctggc cacgcgggga gcgccggaag cccacctgat catggccacc
     2161 gatgcgcggc tcgacgccat tctctacggc tgcatactgg cgctggtcgc caacccctgg
     2221 ctcgcacctc cacggcaacg ctcttcgcgc cgcgatgcgg tgattgccat cgcctgcaca
     2281 ctgatgctgc tgggctcgtt gctggagcgc gatccgctgt tccgggtcac gctgcgctac
     2341 acgctgcagt gcctcgcggt gtccgggctg ctttacctgg cggtagcacg tgccgccgat
     2401 gcgcccttcc gctggctcaa cctgcgcccc gtcgtctacg tcgggaccat ttcctacagc
     2461 atctacctca cgcatcacgt gctgctgttc gctgccgccc gttaccttcc cgcgacgcat
     2521 cccttgatcg tcgcggtgat cgccgctctg ctgacgctaa ccgtggctga actgatgcgc
     2581 cgccacgttg aagaaccctg cgcacggttg cggcgaaggc tgcaccacgc caggacccga
     2641 cctgctccgc tgcagtcttc cacccaggtg accctgcaat gaccgctgcc actgccctgg
     2701 ccgatctgat gccggatgca attcccgacc acaaggcccg gcccaatgcc gcggatttcg
     2761 agcgcacacc ggagcaagca ctcgaagcgg cagcttcgca gccggggctg ctgctggtgg
     2821 atctggacga aaccctgttc ctgcacaatt cgaccgaact gttcctcgaa tgcgcccggc
     2881 cgggcatcct ggccctgctg atcctgcgcc tgctcgacgg gttgcgacca tggcgctaca
     2941 ccggcggcat cgacacgcgc gacaactggc gcgtcggcgt catcgctctc ctgtttccgt
     3001 ggacctggct cgtctggcgc cagcgcgcgt gccggtatgc ggcggatcac gtgaacatgc
     3061 ccttgcgcga tgccttgctg cgccgctcgg tcgcaccggt catcgtcacc gccggattcc
     3121 tgccgatcgt gcgcacgttg tgccgtgcca tggaactcgg cgacacacgc atcatcggct
     3181 gccgcctgtt cgcgctggac gaccgccgcg gcggcaagct gttgctggcg aaacgcacgc
     3241 ttggaatgca aagcatctgc gacgccacgg tcatcaccga ttcgcccagc gacctgccgg
     3301 tgctgcatgc ctgctccaat cgcatgctgg tgcgctggcc gcaagcgcgc ttcgtgcctg
     3361 ccttcgatcg catgtacatg ccgggccagt acatcgcgcg ggtgaagcat gtcggtcagc
     3421 gctacatcct gcgcggcatc ctgcaggaag atttcgcctt ctgggtgctc gcgtccatcg
     3481 ggctcgccgc cgcgccggca tggcactgca ccggcctgct gctgctgctg gtttcgttct
     3541 gggcgatcta cgagcgcggc tatgtcgaca atgacgccac cggcgcgcgc cacgagagcg
     3601 accccaagct cagcccggaa tttcacacca gcccggtggc aacgccgctg ctggcaccat
     3661 ggatatgggc gacagcgtcc ggcatcctcg gcgtccttgc gctgtggcac ccggcaagtc
     3721 cgccaccgga tgcctacctg cgctgggcgg tggtgctggt cgctgcgcac gcctggttct
     3781 ggatctacaa ccgcgtcgac aaaggcaccc gcgtgtggct ctattccggg ctgcaggtcg
     3841 gccgcaccgc ggctttcgtc gcgctggttc cggtctcgga aatcggcgtg gctgcgcttg
     3901 ccgcccacgt gtttgcacgc tggacgccgt actacttcta ccggcacggc agcaaccgct
     3961 ggctgaccga gcagttccat ctcacccgcc tgatgatgtt cctgtggctg gccgtgatgc
     4021 tgggactggc caccggtctg gacagcgtgg ccaatggcac gggcgccatg ctgctggcct
     4081 ggaacctgtt ccgtgcgcgc cacgaactgc gcgctgccgc gcgacacgct catcgcatcg
     4141 accgggagca gccatgaaag ccgagcgcgt cagcatctgc cttgccacct accgccggcc
     4201 ggagcagctg gcggccctgc tggacgatct gctgcagcag acacgcctgc ccgacgagat
     4261 cgtcgtgatc gacaacgaca gcgccgccag cgcccgggca gtggtcgaag ccttgcccgc
     4321 tcacgctgcc tgtccgatcc gctaccaggt ccagcccgag aaaaacatct cgattacccg
     4381 caatcttacc gtcgctggcg cttccggcaa ctggctggcc ttcatcgatg acgacgagcg
     4441 cgcaccgcgt gcgtggttgc agcaactgct cgatgcgcgc cgccgccacg atgccgatgg
     4501 tgtgctcggc ccggtgctgc cggtcattcc cgcgtcggcg cctggatgga tacgccgcgg
     4561 ccgcttctat gagtggccga g
//
LOCUS       DASZZC010000482         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_100617,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000482 DASZZC010000000
VERSION     DASZZC010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100617"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="mutY"
                     /locus_tag="VHE37_10230"
     CDS             complement(<1..790)
                     /gene="mutY"
                     /locus_tag="VHE37_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVT35954.1"
                     /translation="MKTRTDFSARLLAWFDQHGRHDLPWQHPREPYRVWLSEIMLQQT
                     QVTTVMPYFERFLARFADVQALAAAPLDDVLALWSGLGYYARARNLHACAQTVVREHG
                     GEFPRDIETMMALPGIGRSTAGAILAQAHGQRHAILDGNVRRVLSRHAAVPGWTGEPA
                     VQKKLWAIAESLLPQARLPDYTQALMDLGATVCTARKPACDACPVRADCAARTQQRVA
                     EFPAAKPKRARPQRRSLMLLIENARGEILFERRAAAGIWADCGAC"
     gene            complement(774..1328)
                     /locus_tag="VHE37_10235"
     CDS             complement(774..1328)
                     /locus_tag="VHE37_10235"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVT35955.1"
                     /translation="MRRAGIAALMLSLSAQAAEPAVTVTRADGVYSVQMHAQLDVTPA
                     HADAVFTRYENLPRINPAVEVVQRRGGGADATRLYTQVRVCVAFFCKHLHQVQDMRRA
                     QHDGIYEIDAQVLPGQGELKRGAAHWRIAPCGNGSCLEFSAQLQPDFWVPPLIGPWLI
                     QRKLREEAEVTVQGIERVAHEDAH"
     gene            complement(1331..3484)
                     /locus_tag="VHE37_10240"
     CDS             complement(1331..3484)
                     /locus_tag="VHE37_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HVT35956.1"
                     /translation="MAKPLKILLIIVGVIVALFAALAVALPLFFNPNDHRDDIARAVK
                     EATGREFSAGAIQLHVFPWLAVELHDITLGNAAGFGSEPFAKLADARAGVRLLPLLFG
                     GRIAVSTVTVHGLALNLQKDRSGKNNWSDLSQNKEEKKEPQPAKVEAKGNLEIKEFAV
                     GGVDIEDGAVAYSDAQAGKNYRIEHVNLKTGALELGEPVDFKLGLTAVSSAPALTAQI
                     TLAAKLAADTKAQQYKLDDLSFTVDASGAGVPGGKQQVTLSGNASYDGKADELKIPGA
                     KLGAAGISIQADISGKGLTGDSPKLSGPIRVAEFSPREVLGKLGQKIETTDANALKSM
                     SLSANYSGTFKSALLDAVALKLDQTTASGSIDVRDFATADIRFALKVDQFDADRYSSP
                     RGKAQAQAEKKPVDSKTLNETELPLDAIADLNAQGTLDVGQLKVSGLTLTGASVKLDG
                     ARGQVKNLDVGAKLYSGSITMHTKVTPGAKPQLATATKLAGLQTGPFLHDFMGKDILS
                     GVGNFDLDVSGSGKTVGDLRKSLDGNVALKLDNAVVKGFNMAAILRKGQALLAGNSNY
                     QESAPEETDFSAISATGKIANGVLTSNDLAGASPAFRLAGAGKVDLANETIDYLVKPT
                     IVETSTGQNGKSLDQLRGVTIPIQVSGTFSAPSYKLDVASALQQKALSKVNEKIDANK
                     AKLQDKVNNALGKLFGNRQQQPAAAPAATPSSSQH"
     gene            complement(3711..4451)
                     /locus_tag="VHE37_10245"
     CDS             complement(3711..4451)
                     /locus_tag="VHE37_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35957.1"
                     /translation="MNLALPAGTVRRLSGLAVLIVCAMLASAQAPLRAAGADGGALSL
                     IAVPETSRIASGSAVPVARFELANLTGQPQQLSALTLHLSNPALFDAIVLTRPGMPPL
                     RIDHPQDTAQFELPASLQLAPGASATFAVIARAATPALTALGDRWLLLRENLLARSVG
                     APPPDARPLAQALVAAALIILLVALLPRRWLTATLAAAAFAAGCSAVPPTLSAHASDM
                     LISAVSSEHDARAGAPARPVRVSTITRL"
BASE COUNT          676 a         1509 c         1596 g          804 t
ORIGIN      
        1 ggcaggcacc acagtccgcc cagatgccgg cggcggcgcg gcgctcgaac aggatctcgc
       61 cgcgcgcgtt ctcgatcagc agcatcaggc tgcggcgctg cgggcgcgcg cgttttggtt
      121 ttgcggcggg gaattccgcc acgcgctgct gcgtgcgcgc ggcgcaatcc gcgcgcaccg
      181 ggcaggcgtc gcacgcgggt ttgcgcgcgg tgcagacggt ggcgcccagg tccatcagcg
      241 cctgcgtgta gtcgggcagg cgcgcctgcg gcagcagcga ttcggcgatg gcccacaatt
      301 ttttctgcac ggccggctcg ccagtccagc cgggcacggc ggcgtgacgc gacagcacgc
      361 ggcgcacgtt gccgtcgagg atcgcgtgcc gctgcccgtg tgcctgcgcc aggatcgcgc
      421 cggcggtgga ccggccgatg ccgggcagcg ccatcatcgt ttcgatgtcg cgcggaaatt
      481 cgccgccgtg ttcgcgcacg acggtctgcg cgcaggcgtg caggttgcgc gcgcgcgcgt
      541 agtaacccag gcccgaccac agcgcgagca cgtcatccag cggcgcggcg gccagcgcct
      601 gcacatcggc aaagcgtgca aggaagcgct cgaagtaggg catgacggtg gtcacctgcg
      661 tctgctgcag catgatttcc gacagccaca cgcggtaagg ctcgcgcggg tgttgccagg
      721 gcagatcgtg gcggccgtgc tggtcgaacc aggccagcag gcgcgcagaa aaatcagtgc
      781 gcgtcttcat gcgccacccg ctcgatgccc tgcaccgtca cttcggcttc ctcgcgcagc
      841 ttgcgctgga tcagccacgg accgatcagc ggcggcaccc agaagtccgg ctgcaactgc
      901 gccgagaatt ccaggcagct gccgttgccg cagggcgcga tgcgccagtg cgcggcgccg
      961 cgtttgagct caccctgccc gggcagcacc tgcgcgtcga tctcgtagat gccgtcgtgc
     1021 tgtgcacggc gcatgtcctg cacctggtgc aggtgcttgc agaagaaagc cacgcacacg
     1081 cgcacctgcg tatacaggcg cgtggcgtcc gcgccgccac cgcgccgctg cacgacttcc
     1141 actgcgggat tgatgcgcgg caggttttcg tagcgggtga acacggcgtc cgcatgcgcc
     1201 ggcgtcacat cgagctgcgc atgcatctgc accgagtaca cgccatccgc gcgcgtcacg
     1261 gttaccgccg gctcagcggc ttgcgcagac aggctcagca tcagcgcggc gataccggca
     1321 cgcctcactg ctagtgctgt gatgacgacg gcgtggctgc gggggcagct gcgggctgct
     1381 gctgccggtt gccgaacagc ttgcccagcg cgttgttcac cttgtcctgc agcttggcct
     1441 tgttggcgtc gatcttctcg ttgaccttcg acaacgcctt ctgctgcagc gccgaggcca
     1501 cgtcgagctt gtagctcggc gcgctgaagg tgccggagac ctggatcggg atggtcacgc
     1561 cgcgcagctg gtcgagcgat ttgccgttct ggccggtgct ggtctcgacg atggtcggct
     1621 tcaccaggta atcgatcgtc tcgttggcca ggtccacctt gcccgcgccg gccaggcgga
     1681 aggcggggct ggcgccggcc aggtcattcg aggtgagcac gccgttggcg atcttgccgg
     1741 tggcgctgat ggcggagaag tcggtctctt ccggcgcgct ttcctggtag ttcgaattgc
     1801 cggcgagcag cgcctggccc ttgcgcagga tggctgccat gttgaagccc ttgaccacgg
     1861 cgttgtcgag cttgagcgcg acattgccgt ccagcgactt gcgcaggtcg ccgaccgtct
     1921 tgccgctgcc ggatacgtcg aggtcgaagt tgccgacgcc ggagaggatg tccttaccca
     1981 tgaagtcgtg caggaacggc ccggtctgca ggccggccag tttggtggcc gtcgccagct
     2041 gaggtttcgc accgggcgtg accttcgtgt gcatggtgat gctgccgctg tacagcttgg
     2101 cgcccacatc gaggttcttc acctggccgc gcgcgccgtc gagcttgacg ctggcgccgg
     2161 tcagcgtgag gccagagacc ttgagctggc ccacgtcgag cgtgccttgc gcgttgagat
     2221 cggcaatcgc gtccagcggc agctcggtct cgttcagcgt cttgctgtcc accggtttct
     2281 tttcagcctg cgcctgcgcc ttgcccctgg gcgagctgta gcggtcggca tcgaactggt
     2341 ccaccttgag cgcgaagcgg atgtcggccg tggcgaaatc gcgcacgtcg atgctgccgc
     2401 tggcggtggt ctggtcgagc ttgagcgcca ccgcatcgag cagcgcgctc ttgaaggtgc
     2461 cgctgtagtt ggcggagagg ctcatgctct tgagcgcgtt ggcgtcggtg gtctcgatct
     2521 tctgcccgag cttgcccagc acctcgcgcg gcgagaactc ggccacgcgg atcggccccg
     2581 agagtttcgg cgagtcgccg gtgaggccct tgccgctgat gtcggcctgg atgctgatgc
     2641 cggcagcgcc gagcttggcg ccgggaatct tcagctcgtc ggccttgccg tcatagctgg
     2701 cgttgccgga cagcgtcacc tgctgcttgc cgccgggcac gccggcgccg ctggcatcga
     2761 cggtgaagga aagatcgtcg agcttgtact gctgcgcctt ggtgtcggcc gcgagcttcg
     2821 ccgccagcgt gatctgcgcg gtgagcgcag gcgcgctcga caccgcggtg aggccgagct
     2881 tgaagtccac cggttcgccc agctccagcg cgccggtctt caggttcacg tgttcgatgc
     2941 ggtaattctt gccggcctgc gcatcgctgt acgccaccgc gccgtcctcg atgtcgacgc
     3001 cgcccacggc gaattccttg atctcgagat tgcccttggc ctccaccttg gccggttgcg
     3061 gttccttctt ctcttccttg ttctgcgaca ggtcgctcca gttgttcttg ccgctcctgt
     3121 ccttctgcag gttcagcgca aggccgtgca cggtcaccgt gctgacggcg atgcgtccac
     3181 cgaacagcag cggcagcagg cgcacgccgg cgcgcgcatc ggccagtttt gcaaacggct
     3241 cgctgccgaa gccggcggcg ttgcccagcg tgatgtcgtg cagttccacc gccagccacg
     3301 ggaacacgtg cagctggatg gcgccggcgg agaattctcg gccggtggct tccttcaccg
     3361 ccctggcgat gtcgtcgcgg tgatcgttcg gattgaagaa caagggcagc gcaaccgcca
     3421 gcgccgcgaa cagcgcgacg atgacgccga cgatgatgag caagatcttc agcggcttgg
     3481 ccatggcagg agcagaagcg gagtgaagtg gggcggggcg atagtgtact ggcgccggct
     3541 gactacacag acgctgcaat gcccctgccg ggttcccgcc gcggccattt tttgtttgac
     3601 cgcaccgccc atccggctgt tattaattat cttattaaat taattatgat taataattta
     3661 taacatgcca ggggcgggtg cggttggcat cacccgcggc ctgcgcggcg ttacaggcgc
     3721 gtgatggtcg atacgcgcac gggccgcgcc ggtgcgccgg cacgcgcgtc gtgctcgctg
     3781 ctgacggcgg agatcagcat gtcggacgca tgcgcgctca aggtcggcgg aactgcgctg
     3841 cagccagcgg cgaacgcggc agccgccagc gtggcggtga gccagcggcg cggcagcaat
     3901 gcgacgagca ggatgatcag cgcagcggcg accagcgcct gcgccagcgg gcgcgcatcc
     3961 ggcggcggcg cgcccaccga tcgcgccagc aggttttcgc gcagcagcag ccagcggtcg
     4021 cccagcgcgg tgagtgccgg cgtggccgcg cgcgcgatca ccgcgaaggt cgcgctcgcg
     4081 ccgggcgcga gctgcagcga ggcgggcaac tcgaactgcg cggtgtcctg cggatgatcg
     4141 atgcgcagcg gcggcatgcc cggccgtgtg agcacgatgg cgtcgaacag cgcggggttg
     4201 gacaggtgca gggtcagcgc cgacagctgc tgcggctgtc cggtgagatt ggccagctcg
     4261 aaacgcgcca ccggcacggc gctgcccgag gcgatgcggc tggtctccgg cacggcgatc
     4321 agcgacagcg cgccgccgtc cgcgcctgcc gcgcgcagcg gggcctgcgc cgacgccagc
     4381 atggcgcaga cgatcagcac cgccagtccg ctcaggcggc gcacggtgcc tgcgggcagg
     4441 gcaaggttca ccaggaattt cccttcgatc tggaaaaccg gcgcatgcta accgcgccgc
     4501 atgacgcccg catgagaggt ttcctcaaaa aaattgctcc atgtgaaatc gcgggcatga
     4561 aaaaaggcgg cacgaggccg cctcg
//
LOCUS       DASZZC010000483         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_617482,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000483 DASZZC010000000
VERSION     DASZZC010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617482"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VHE37_10250"
     CDS             <1..1059
                     /locus_tag="VHE37_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVT35958.1"
                     /translation="RLIVIVQRGGMDGLAAVPAYGESPFASLRGALDTGSALKLDGTF
                     ALHPALAQCAQMYHSGEFAVVHAVAPPYHGRSHFEAQDCLENGSEMPHGQQDGWLNRA
                     VQAMPGAEGLAVASAMPLILRGDGAVETWSPSPLPEASEDLLARVAKLYARDPALAPA
                     FQRAMGADDMPAAKMEGDGAATRLPAAMRDAARFMHGRNGLRVVVLQDGGWDTHARQG
                     AADGQLAGKLRGLDNALDAARNALGPLWKETIVVVATEFGRTVRVNGSGGTDHGTGGV
                     MLLAGGAIAGGKIFGDWPGLASRNLLDGRDLKPTTDSRAVFKTLLRDHLGLSEAALDA
                     KVFPASSGIKPLPGLVRA"
     gene            complement(1234..>1713)
                     /locus_tag="VHE37_10255"
     CDS             complement(1234..>1713)
                     /locus_tag="VHE37_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HVT35959.1"
                     /translation="HCVCSLFISRWDQATAALPDGLRNRVGIAIGRRCRAELETCNPQ
                     RWRRVTAAGGHAPRLVFASTGTKDPAASDVLYVEALIAAGTITTVPEETLLAFARHGS
                     ARLHTGDEDEVLARMAQHGCVLDRLAAQLQQQGCESFTDAWRKLLTAVANKGAAATG"
BASE COUNT          263 a          593 c          594 g          263 t
ORIGIN      
        1 cggctgatcg tcatcgtgca gcgcggcggc atggatggcc tggcggcggt gccggcttac
       61 ggcgaatcgc cgttcgcctc gctgcgcggc gcgctcgaca ccggcagcgc gctcaagctc
      121 gacggcacct tcgcgctgca cccggcgctg gcgcagtgcg cgcagatgta ccactccggc
      181 gaattcgccg tggtgcacgc ggtggcgccg ccgtatcacg gccgctcgca cttcgaggcg
      241 caggactgcc tggagaacgg cagcgagatg ccgcacggcc agcaggacgg ctggctcaac
      301 cgcgcggtgc aggcaatgcc tggcgcggaa ggactggccg tcgcctccgc catgccgctg
      361 atcctgcgcg gcgatggcgc agtggaaacc tggtcgccat cgccgctgcc ggaggcgagc
      421 gaagacctgc tggcgcgcgt ggcgaaactc tacgcgcgcg atcccgcgct ggcgcccgcg
      481 ttccagcgcg cgatgggcgc cgacgacatg ccggcggcca agatggaagg cgatggcgca
      541 gccacgcgcc tgccggcggc catgcgcgat gccgcgcgct tcatgcacgg gcgcaacggt
      601 ctgcgcgtgg tggtgctgca ggacggcggc tgggacacgc acgcgcgcca gggcgcggcc
      661 gacggccagc tcgccggcaa gctgcgcggc ctggacaatg cgctcgatgc cgcgcgcaac
      721 gcgctcggac cgctgtggaa ggaaacgatc gtggtggtcg ccaccgagtt cggccgcacc
      781 gtgcgcgtca acggcagcgg cggcaccgat cacggcaccg gcggcgtcat gctgctcgcc
      841 ggcggcgcca tcgccggcgg aaaaattttc ggcgattggc ccgggctggc atcgcgcaac
      901 ctgctcgacg gccgcgacct caagcccacc accgacagcc gcgccgtgtt caagacgctg
      961 ctgcgcgatc acctgggtct gagcgaagcg gcgctcgacg caaaagtgtt tcccgccagc
     1021 agcggcatca agcctctgcc tggcctcgtg cgcgcttgaa tgccagcggc cgcgaccgat
     1081 gcggccgcat attccctctg cttcgccgtt gttgccggtg cagacggggt caccgcacct
     1141 gcattttgaa tgttattaaa tatatcataa ttataatttc gttttatttt ttataacata
     1201 tcgggagagg gtgcggtcag gccaaaaaag ctgtcagccc gttgcggccg cgcccttgtt
     1261 cgccaccgcg gtcagcagtt tccgccacgc atcggtgaag gactcgcagc cctgctgctg
     1321 caactgcgca gcgagccggt cgagcacgca gccgtgctgc gccatgcgcg cgagcacctc
     1381 gtcctcgtca ccggtatgca ggcgcgcgct gccgtgccgt gcaaacgcca gcagcgtttc
     1441 ctcgggcacc gtggtgatgg tgccggcggc aatcagcgct tcgacgtaga gcacgtcgga
     1501 tgccgcgggg tccttggtgc cggtgctggc aaacaccagc cgcggcgcat gcccgccggc
     1561 cgcggtcacc cggcgccagc gttgcgggtt gcaggtctcc agctcagccc ggcagcggcg
     1621 gccgatggcg atgccgacac gattgcgcag cccgtccggc aacgctgccg tggcctggtc
     1681 ccagcggctg atgaacagcg agcacacgca gtg
//
LOCUS       DASZZC010000484         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_233665,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000484 DASZZC010000000
VERSION     DASZZC010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233665"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHE37_10260"
     CDS             <1..235
                     /locus_tag="VHE37_10260"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVT35960.1"
                     /translation="ARGVPNPIGGLQNHQHGTLRCGLDETTSVLDAHCRFHGVPNLYV
                     ADGSVLPGSGGYNPTLTIQAMAWRTAQAMLEAR"
     gene            complement(363..1058)
                     /locus_tag="VHE37_10265"
     CDS             complement(363..1058)
                     /locus_tag="VHE37_10265"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HVT35961.1"
                     /translation="MNDSSFLIIGHRGAAGCAPENTLLALERGIRQGARAVEFDVQLH
                     PSGELFLLHDLTLDRTTNGKGLAERLPWDKLRALDAGGGQQIPTLGEALELVERQVLV
                     NVELKTWNGTGAAVAQVLREHIADGWSPADFLVSSFHLPELFEFKQAAPEIPIGVLTC
                     GVPLDWAAVTTELGAQALNISAEFVDAQLVDDAHKRGVKVYVYTVNRAEDVALLQRLG
                     VDGVFTDFPERFF"
     gene            complement(1086..2513)
                     /gene="pyk"
                     /locus_tag="VHE37_10270"
     CDS             complement(1086..2513)
                     /gene="pyk"
                     /locus_tag="VHE37_10270"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HVT35962.1"
                     /translation="MSRRTKIVATLGPATDAPGVLPALLSAGVDVVRMNFSHGVAADH
                     ARRAREVREAAAKLGRDVAVLADLQGPKIRIESFAKGPVELREGQPFRLDTALGASAG
                     DERVVGCAYADLPRDVAPGDTLLLNDGAISLRVDAVEGTVIDTTVLVGGLLSNRKGIN
                     RQGGGLSAAALSSKDREDIRHAVALDADFIAVSFPRDAADMLEARKLVSDAGGSAQLV
                     AKIERAEALDALEEIIAASDAVMVARGDLGVEIGDAELPAWQKRIIAAARDKNRMVIT
                     ATQMMESMITNPIPTRAEVLDVANAVLDGTDAVMLSAETASGRYPVKVIDTMVRVCLG
                     AERVDGVGKVGRKLEGHFQKTDEAIAMATTWTAQNMRAQAIISLTESGSTALLMSRTD
                     TNIPIYALTRHERTRRRMCLVRNVYPLDFAPSQLETRAPIAEAVALLESRGLVRKGDR
                     VLATKGDFTGPGGTNTMKIVTVGES"
     gene            2609..>2917
                     /gene="purT"
                     /locus_tag="VHE37_10275"
     CDS             2609..>2917
                     /gene="purT"
                     /locus_tag="VHE37_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648124.1"
                     /note="non-folate utilizing enzyme, catalyzes the
                     production of beta-formyl glycinamide ribonucleotide from
                     formate, ATP, and beta-GAR and a side reaction producing
                     acetyl phosphate and ADP from acetate and ATP; involved in
                     de novo purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase 2"
                     /protein_id="HVT35963.1"
                     /translation="MTTLGTPLAANATRVLLLGSGELGKEVVIELQRYGVECIAVDRY
                     AHAPAMQVAHRSHVIRMLDGAEIRRVIEAENPHYVVPEIEAIHTPTLLELEREGRTVI
                     P"
BASE COUNT          470 a          992 c         1000 g          455 t
ORIGIN      
        1 cgcgcgcggc gtgcccaacc ccatcggcgg attgcagaac caccagcacg gcacgctgcg
       61 ctgcgggctg gatgaaacca ccagcgtgct cgacgcgcac tgccgcttcc acggcgtgcc
      121 caatctctac gtggccgacg gctcggtgct gcccggctcg ggcggctaca accccacgct
      181 caccatccag gcaatggcct ggcgcaccgc gcaggccatg ctggaagcac gctgagggcg
      241 aaaaatgctc caaccgcacc gcggattttt gtgttataaa ttatttatca aatgtaaaaa
      301 tatataataa ttaataacac gtaggatggc ggtgcggtgg catcaaaaaa cgggcgctgc
      361 ggtcagaaaa accgctcggg gaaatcggtg aagacgccgt cgacgcccag ccgctgcagc
      421 agcgccacat cttccgcgcg gttgaccgtg tacacgtaca ccttcacgcc gcgcttgtgc
      481 gcatcgtcga ccagttgcgc gtcgacgaac tcggccgata tgttgagcgc ctgcgcgccc
      541 agctcggtgg tgacggcggc ccagtccagc ggcacgccgc aggtgagcac gccgatggga
      601 atctccggcg ccgcctgctt gaactcgaac agttccggca ggtggaacga cgacaccagg
      661 aaatcggcgg gactccagcc gtcggcgatg tgctcgcgca gtacctgcgc caccgcggcg
      721 ccggtgccgt tccaggtctt cagctcgacg ttcaccagca cctgccgctc gaccagttcc
      781 agtgcttcgc cgagcgtggg aatctgctgg ccgccgccgg cgtcgagcgc gcgcagcttg
      841 tcccacggca ggcgctcggc caggcccttg ccgttggtgg tgcggtccag cgtgaggtcg
      901 tgcagcaaaa acaattcgcc ggagggatgc agctgcacgt cgaactccac cgcgcgcgcg
      961 ccctggcgga tgccgcgctc cagcgcgaga agggtgtttt caggcgcgca gccggcggcg
     1021 ccgcggtgac cgatgatgag gaagctgctg tcgttcatga cgccctcccg cagaaatgga
     1081 aacagtcagc tttcgcccac cgtgacgatc ttcatggtgt tggtgccgcc cgggccggtg
     1141 aaatcgccct tggtcgccag cacgcggtcg cccttgcgca ccagtccgcg gctctccagc
     1201 agcgcgacgg cctcggcgat cggcgcgcgc gtttccagct ggctgggcgc gaaatccagc
     1261 gggtagacgt tgcgcaccag gcacatgcgc cggcgcgtgc gctcgtggcg cgtcagcgcg
     1321 tagatcggga tgttggtgtc ggtgcgcgac atcaacagcg cggtggaacc ggactcggtc
     1381 agcgagatga tggcctgcgc acgcatgttc tgcgccgtcc aggtggtggc catggcgatg
     1441 gcctcgtcgg tcttctggaa atggccttcg agcttgcgcc ccaccttgcc gacgccgtcg
     1501 acgcgctcgg cgcccaggca cacgcgcacc atggtgtcga tcaccttcac cgggtagcgg
     1561 cccgacgcgg tttccgccga cagcatcacc gcgtcggtgc cgtcgagcac ggcattggcc
     1621 acgtcgagca cctcggcgcg cgtggggatg ggattagtga tcatcgactc catcatctgg
     1681 gtcgccgtga tcaccatgcg gttcttgtcg cgcgcggcgg cgatgatgcg tttctgccag
     1741 gccggcagct cggcgtcgcc gatctccacg ccgaggtcgc cgcgcgccac catcaccgca
     1801 tcggaggcgg cgatgatctc ctccagcgca tcgagtgcct cggcgcgctc gattttcgcc
     1861 accagctgcg cgctgccacc ggcgtcgctc accagcttgc gcgcctccag catgtcggcg
     1921 gcatcgcgcg gaaaactcac cgcgatgaaa tcggcatcga gcgccaccgc gtggcggatg
     1981 tcctcgcggt ccttggacga cagcgccgcg gcggacaaac cgccgccctg gcggttgatg
     2041 cccttgcggt tggacagcag gccgccgacc agcacggtgg tgtcgatgac agtgccctcg
     2101 accgcgtcga cgcgcaggct gatggcgccg tcgttgagca gcagcgtgtc gccgggtgcc
     2161 acgtcgcgcg gcaggtcggc gtaggcgcag ccgacgacgc gctcgtcgcc ggcgctggcg
     2221 cccagcgcgg tgtcgaggcg gaacggctgg ccctcgcgca gttccaccgg gcccttggcg
     2281 aaggattcga tgcggatctt cggcccctgc aggtcggcca gcaccgcgac atcgcggccg
     2341 agcttcgcgg ccgcctcgcg cacctcgcgc gcgcgccgcg cgtggtcggc cgcgacgccg
     2401 tgggagaaat tcatgcggac cacgtcgacg ccggcggaga gcagcgcggg cagcacgccc
     2461 ggcgcgtcgg tggcggggcc gagggtggcg acgatcttgg tgcggcggct catggaatgc
     2521 gggcgggggg ctggggcagg aaagcttact ccgccgcggc gcgcgataaa attgccgcgc
     2581 cggccaacgg cgccatgggg atgaacaaat gaccacgctg gggacacctc tggccgccaa
     2641 tgccacgcgc gtgctgctgc tgggctcggg cgaactgggc aaggaagtgg tgatcgagct
     2701 gcagcgctac ggcgtggagt gcatcgcggt ggaccgctac gcgcacgcgc cggcgatgca
     2761 ggtggcgcac cgctcgcacg tcatccgcat gctcgacggc gcggagatcc gccgcgtgat
     2821 cgaggcagag aacccgcact acgtcgtgcc cgagatcgag gcgatccaca cgcccacgct
     2881 gctcgagctc gaacgcgaag gccgcacggt gatcccg
//
LOCUS       DASZZC010000485         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_491069,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000485 DASZZC010000000
VERSION     DASZZC010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491069"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1779
                     /locus_tag="VHE37_10280"
     CDS             <1..>1779
                     /locus_tag="VHE37_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HVT35964.1"
                     /translation="GMVAGFALWFYTLLLPAVLSGDAAPAWFVSGPWGWAWLRPQALL
                     GTAAWPALAHGTFFSLAANMFCYVAGSIVRGARLHDRLQLVRFLGDDARPPAAARKAA
                     QVEATVGDLQELLERFLGAERTRDVLSGHALRADARADAALLRQTEHLLAGALGASSA
                     RLVLSSMLRGRDMQLEEVIGLLDETSHAIQFNRELLRATLEHLSQGVSVVDAELRLVA
                     WNRRYVELLGYPEALVTVGRPIEELVRYNAQRGLLGSGDTDEQVRRRLEHMRAGHAYT
                     HQRGLPDGSVVEIRGNPMPGGGFVTSYSDVTDYKRSERALQEAKESLETRVDQRTREL
                     AQAKLAAEHADDAKTRFLAAASHDLVQPLNAARLFISSIDRARLDTASAGLVGQVENS
                     LGAAEELLAGLLDISRLDAHAQEVRREHFRLSEVLAPLAAETALLAARHGLKFRYAGC
                     ERVVHSDPRLLRRIVQNFLSNAVRYTVSGRILLGCRRAPGGAVRIEVWDTGPGIAPER
                     QREIFEEFRRLEVRDAHGQRGLGLGLAIVERIARLLDHPLGLRSQPGRGSVFSVTVPR
                     GIATARAAPARRSSQPRGRVAGARVLC"
BASE COUNT          236 a          634 c          636 g          273 t
ORIGIN      
        1 ggcatggtgg ccggcttcgc actctggttc tacaccctgc tgctgccggc agtgctgtcc
       61 ggcgacgcgg ccccggcgtg gtttgtctcc ggaccgtggg gatgggcatg gctgcggccg
      121 caggcgctgc tcggaaccgc ggcctggccg gccctggcac acggcacctt cttcagcctg
      181 gcggccaaca tgttctgtta cgtggccggc tcgatcgtgc gcggtgcgcg cctgcacgat
      241 cgcctgcagc tggtgcgctt tctcggcgac gacgcacgcc ctccggcggc ggcacggaaa
      301 gccgcgcagg tcgaggccac cgtcggcgac ctgcaggagc tgctggagcg cttcctcggc
      361 gccgagcgta cccgcgacgt gctgtccggc cacgccttgc gcgctgacgc ccgcgctgac
      421 gccgcgctgt tgcgccagac cgagcacctg ctggccggcg cgctcggcgc ctcgtcggcc
      481 cggctggtgc tgtcctcgat gctgcgcggg cgcgacatgc agctcgagga agtcatcggc
      541 ctgctggatg aaacctcgca cgccatccag ttcaaccgcg aactgctgcg cgccacgctg
      601 gagcatcttt cgcagggcgt gagcgtggtc gatgccgagc tgcggctggt cgcctggaac
      661 cgccgctacg tcgagctgct cggatatccg gaagcgctgg ttacagtggg ccggccgatc
      721 gaggaactgg tgcgttacaa cgcgcagcgc ggcctgctcg gttccggcga caccgacgaa
      781 caggtgcgcc gccgcctcga gcacatgcgt gccggccacg cctatacgca ccagcgcgga
      841 ctgccggacg gcagcgtggt ggagattcgc ggcaacccga tgccgggcgg cggcttcgtc
      901 accagctatt ccgacgtgac cgactacaag cgcagcgagc gcgcgctgca ggaagccaag
      961 gagtcgctgg agacgcgcgt cgaccagcgc acgcgtgaac tggcgcaggc caagctcgcc
     1021 gccgagcacg ccgacgatgc caagacacgc ttcctcgcag ccgcttcgca tgacctggtg
     1081 cagccgctca atgcggcacg cctgttcatc tcgtcgatcg atcgcgccag gctcgacacc
     1141 gcgtccgccg ggctggtcgg ccaggtggag aattcactgg gcgcggccga ggaactgctg
     1201 gcgggcctgc tcgacatctc gcggctggac gcgcatgcgc aggaagtgcg gcgcgaacat
     1261 ttccggctga gcgaagtact ggcgccgctg gcggctgaaa cggcgctgct ggccgcccgg
     1321 catggactga agttccgcta tgccggctgc gagcgcgtgg tgcacagcga tccgcgcctg
     1381 ctgcggcgca tcgtgcagaa cttcctgtcc aacgccgtgc gctacaccgt gtcgggccgc
     1441 atcctgctgg gctgtcgccg cgcgcccggc ggcgccgtgc gtatcgaggt ctgggacacg
     1501 ggccccggca tcgcgcccga gcgccagcgc gaaatcttcg aggagttccg ccgcctggaa
     1561 gtgcgcgacg cccatggcca gcgcggcctc ggcctgggtc tggccatcgt cgagcgcatt
     1621 gctcgcctgc tcgaccatcc gctggggctg cgctcgcagc cgggccgcgg cagcgtgttc
     1681 agcgtgacgg tgccgcgcgg catcgccacc gcccgcgcgg cgccggcgcg gcgcagttca
     1741 cagccgcgcg gccgcgtggc gggtgcgcgc gtgctgtgc
//
LOCUS       DASZZC010000486         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_945255,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000486 DASZZC010000000
VERSION     DASZZC010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945255"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VHE37_10285"
     CDS             <1..931
                     /locus_tag="VHE37_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35965.1"
                     /translation="PDVVLAAMRAGARDFFVLRKDEANVAASLGRLLRRTAQAAQSGR
                     GAPRGKLFTVLSGHPHEGIAFLAEHLSLACAEALPRSERVLLLDAATPAGAAAIFLNI
                     AQSYSVLDAVNDVYRCDQTLVDTAFPKHSSGLYLLSLPEDFVGRPQVNTDEFLKLLQV
                     LRGLFAVIVVALDGHVAMAAIKGVVAQADRSMLMTDQSIIKSRHSKYLLRALRLEDCP
                     LDRMALVVDNYRRRLGLEPQNLAELLDLPLLGTLSTDAAARIQAMNSGEPLFTVAPKD
                     VYCGDIRRLAAALLAGQARVEEAPKSALAKLFG"
     gene            958..2181
                     /locus_tag="VHE37_10290"
     CDS             958..2181
                     /locus_tag="VHE37_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HVT35966.1"
                     /translation="MSSSADPQITRFRLSDQYQQLKRNFHRHLIQQIEERNLDIDRWD
                     AGKIDRFVTEQMRRYVVDQRLPVNQRESEALAGDARDELVGYGPIQALVDDDGVADIV
                     VNGPNRVFIERDGKLTTAAVRFVDDAHVVRVIQRILSPIGRRVDESTPMVDARLPDGS
                     RVNAIIPPIALDGPCLSIRKFRKKALTAEDLLRYGSITQAQIDYLQARVRGRTNLIVV
                     GGTGSGKTTFLNLLSQWIPPAERIITIEDAAELRLEHEHVVRLETRPPNLEGERAVTA
                     RALVRNALRMRPDRIIVGEVRGDEVLDMLQAMNTGHDGSMTTIHANSTRDAIHRIELL
                     AGFAGYQGSEVTFRGQVGSALQLLVQVARLVGGERRVTAVTEVNGLHDGELVLRDVFR
                     YDLERAVHVDLRGGA"
     gene            2178..>2433
                     /locus_tag="VHE37_10295"
     CDS             2178..>2433
                     /locus_tag="VHE37_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35967.1"
                     /translation="MNPLMLVFVATLLLVGTAVFLLLRASERDKHDDVQLRLRVLGGD
                     DAAAAMVNYQPRDAKLTNPLLRAACRLVWRTGAEVEPDTVS"
BASE COUNT          367 a          865 c          820 g          381 t
ORIGIN      
        1 ccccgacgtc gtgctggccg cgatgcgcgc cggtgcgcgc gatttcttcg tgctgcgcaa
       61 ggacgaggcc aacgtcgccg cttcgctcgg ccgcttgctg cgccgtaccg cgcaggcggc
      121 gcagtccggc cgcggcgctc cgcgcggaaa actgttcacc gtgctgtcgg gtcatccgca
      181 cgagggcatc gccttcctgg ccgagcacct gtcgctggcc tgcgccgagg cgcttccgcg
      241 cagcgagcgc gtgctgctgc tggatgcggc cactcctgcc ggtgctgccg ccatctttct
      301 caacatcgcg cagtcgtaca gcgtgctcga cgcggtgaac gacgtttacc gctgcgacca
      361 gacgctggtc gatactgcat ttcccaagca ttccagcggt ctgtacctgc tgagcctgcc
      421 ggaggatttt gtcggccgcc cgcaggtcaa caccgacgaa ttcctcaagc tgctgcaggt
      481 gctgcgcggc ctgtttgcgg tgatcgtggt ggcgctggac ggccacgtgg cgatggccgc
      541 gatcaagggc gtggtggcgc aggctgaccg cagcatgctg atgaccgacc agtcgatcat
      601 caagtcgcgc cacagcaagt acctgctgcg tgcgctgcgc ctggaggact gcccgctcga
      661 ccgcatggcg ctggtggtcg acaactaccg ccgtcgcctg ggcctggagc cgcagaacct
      721 ggccgaactg ctcgacctgc cgctgctcgg cacgctgtcc accgacgccg cggcgcgcat
      781 ccaggcgatg aactccggcg agccgctgtt cacggttgcg cccaaggacg tctattgcgg
      841 cgacatccgc cgcctggccg cggcgctgct tgccggccag gcccgcgtcg aggaagctcc
      901 caagagcgcg ctggccaagc tgttcggctg agcgcgcacg cgtaacgcga gtcgcccatg
      961 agctcctccg ccgatcccca gatcacgcgc ttccggctgt cggaccagta ccagcagctc
     1021 aagcgcaatt ttcaccggca cctgattcag cagatcgagg agcgcaacct cgacatcgac
     1081 cgctgggatg ccggcaagat cgaccgcttc gtcaccgagc agatgcgccg ttacgtggtg
     1141 gaccagcgcc tgccggtcaa ccagcgcgag tcggaggcgc tggccggcga cgcccgcgac
     1201 gagctggtcg gctacggccc gatccaggcc ctggtcgacg acgatggcgt ggccgacatc
     1261 gtggtcaacg gccccaaccg cgtgttcatc gagcgcgacg gcaagctgac caccgcggcc
     1321 gtgcgcttcg tcgacgatgc gcacgtggtg cgcgtgatcc agcgcattct ttcgcccatc
     1381 ggccgccgcg tcgacgagtc cacgccgatg gtcgacgcgc ggctgcccga tggttcgcgc
     1441 gtcaacgcca tcatcccgcc gattgcgctc gacggcccgt gcctgtcgat ccgcaagttc
     1501 cgcaagaagg cgctgaccgc cgaagacctg ctgcgctacg gcagcatcac ccaggcgcag
     1561 atcgactacc tgcaggcgcg cgtgcgcggc cgcaccaacc tgatcgtggt cggcggcacc
     1621 ggctcgggca agaccacctt cctcaacctg ctgtcgcagt ggattccgcc ggcggagcgc
     1681 atcatcacca tcgaggacgc cgccgaactg cgcctggagc acgagcacgt ggtgcgcctg
     1741 gaaacgcggc cgcccaatct cgaaggcgag cgcgccgtga ccgcgcgcgc gctggtgcgc
     1801 aacgcgctgc gcatgcgccc ggaccgcatc atcgtcggcg aggtgcgcgg cgacgaggtg
     1861 ctggacatgc tgcaggcgat gaacaccggc cacgacggtt cgatgaccac catccacgca
     1921 aacagtacgc gcgacgccat ccaccgcatc gaactgctgg ccggcttcgc cggttaccag
     1981 ggctcggagg tgacgttccg cggccaggtc ggcagcgcgc tgcagctgct ggtgcaggtg
     2041 gcgcgcctgg tgggcggcga gcgccgcgtg accgccgtca ccgaggtgaa cgggctgcac
     2101 gatggcgaac tggtcctgcg cgacgtgttc cgctacgacc tcgagcgtgc cgtgcacgtc
     2161 gacctgcggg gcggcgcatg aatccgctga tgctggtgtt cgtagccacg ctgctgctgg
     2221 tcggcacggc ggtgttcctg ctgctgcgcg cgagcgagcg tgacaagcac gatgacgtgc
     2281 agctgcgcct gcgcgtgctg ggcggcgacg acgccgccgc ggccatggtc aattaccagc
     2341 cgcgcgacgc caagctgacc aacccgctgc tgcgcgccgc ctgccgcctg gtgtggcgca
     2401 ccggcgccga ggtcgagccc gatacggtgt cgc
//
LOCUS       DASZZC010000487         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_43267,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000487 DASZZC010000000
VERSION     DASZZC010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43267"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2549)
                     /locus_tag="VHE37_10300"
     CDS             complement(<1..2549)
                     /locus_tag="VHE37_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT35968.1"
                     /translation="MRSFNLSEWALANRGLVVYFMVLFGVLGAWSYTHLGQSEDPPFT
                     FPLMVVRTYWPGATAAEVDQEVTDRIEERLQEHEQVDYLASYSRPGESQVFFRVRDDI
                     GYAATQDAYYQVRKKIGDIRQNLPQGVLGPFFNDEFGDTFGNIYALTGPGFSYAELKD
                     YADDIRGQLLRVKDVAKVEFIGVQDEKIEVRLANTKRSELGIPAQAIIDAIQQQNSVN
                     PAGAFELDHDRVYLRTTGAYRSVDDIRNTPISYNGRTFRVGDVAEVMRGYSDPPGPLM
                     RFRGVPCLGIAVSMRKGGDIIRLGQALDQEFARIQSHLPLGLSLDRVNDQPRAVRRGV
                     GEFVKSLVEAVVIVLAVSFFSLGARSGMVVALSIPLVLAMTFAAMKYFGIDLHKISLG
                     SLVLALGLLVDDAIIAVEMMAVKMEQGMTRLKAAAYAYDTTAFPMLTGTLVTAAGFLP
                     IAIARSSTGDYTRSIFEVVTIALLISWVAAVVFVPYLGYKLLPDYHPDPGEEGVHDPY
                     QTRFYRKFRALVTWCVRHRAKVIVITIGAFVAAVVGFKAVPQQFFPPSARLELLVDVK
                     LPEGSSQAAADEAAHRLEKFLNAQQGLESYASYIGTGAPRFILPLDQQLPQAGFAQFV
                     LTSKEVEDRERIRTTLLQNLPTLLPDARTRVLRLENGPPVGWPVQLRVSGPATADHGV
                     LHDYAEQVAAVLRSHPALTNVNLNWDEPSKIVRVIIDQDRARALGVSNQYVAQFLQTS
                     VSGGTITELRERNKLIEVQFRGPPNERVQLSQLASTAIARPDGSSIPLDQVARIEYGY
                     EDGIIWRRNRLPTMTVRADIPGSMQPAEVIGQLQPQLDAISAKLPVGYRLET"
     gene            complement(2549..>2871)
                     /locus_tag="VHE37_10305"
     CDS             complement(2549..>2871)
                     /locus_tag="VHE37_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35969.1"
                     /translation="EVRVSMADADDAVKLGMTATVIVGQKLDARSFIIPMSALGGSKE
                     QPAVWIVSDGKTHQVPVQVLRYTETGAVVSGALDAAMNMVSAGIHLMHEGMDVSVLQR
                     QHNG"
BASE COUNT          461 a          972 c          956 g          482 t
ORIGIN      
        1 gtctccagcc ggtagcccac cggcagcttc gcgctgatgg cgtcgagctg cggctgcagc
       61 tggccgatca cttccgccgg ctgcatgctg ccgggaatgt cggcacgcac ggtcatggtc
      121 ggcaggcggt tgcgccgcca gatgatgccg tcctcgtagc cgtactcgat gcgcgccacc
      181 tggtcgagcg gaatgctgct gccgtccggg cgtgcgatgg cggtgctggc cagctgcgac
      241 agctgcacgc gctcgttcgg tgggccgcgg aactgcacct cgatcagctt gttcctctcg
      301 cgcagttcgg tgatggtgcc gccggacacc gaggtctgca ggaactgcgc gacgtactgg
      361 ttggatacgc ccagcgcccg tgcgcggtcc tggtcgatga tcacgcgcac gatcttgctc
      421 ggctcatccc agttgagatt gacgttggtc agcgccgggt gcgaacgcag caccgccgcc
      481 acctgctccg cgtagtcgtg cagcacgccg tggtccgctg tcgccgggcc gctcacgcgc
      541 agctgcaccg gccagccgac cggcgggccg ttctccaggc gcagcacgcg cgtgcgcgcg
      601 tcgggcagca gcgtcggcag gttctgcagc agcgtggtgc ggatgcgctc gcggtcctcg
      661 acctctttcg aggtcagcac gaactgggcg aagccggcct gcggcagctg ctggtccagc
      721 ggcaggatga agcgcggcgc gccggtgccg atgtagctgg cgtagctctc cagcccttgc
      781 tgcgcgttga ggaatttctc caggcggtgc gcagcctcgt cggcggcagc ctgcgatgag
      841 ccttccggca gcttgacgtc gaccagcagc tccagccgag ccgacggcgg gaagaactgc
      901 tgcggcaccg ccttgaagcc aacgaccgcg gcgacaaagg cgccgatggt gatgacgatg
      961 accttggccc ggtgccgcac gcaccaggtg accagcgcgc ggaattttct gtagaagcgc
     1021 gtctgatacg gatcgtgcac gccttcttcg cccgggtccg ggtgatagtc cggcagcagc
     1081 ttgtagccga ggtacggcac gaagacgacc gcggcgaccc aggatatgag cagtgcgatg
     1141 gtcaccacct cgaagatcga gcgcgtgtag tcgccggtgc tcgaacgcgc gatggcgatg
     1201 ggcaggaaac cggcggcggt gaccagcgtg ccggtgagca tgggaaacgc ggtggtgtca
     1261 taggcatacg ccgccgcctt caggcgcgtc atcccctgtt ccatttttac cgccatcatt
     1321 tccaccgcga tgatggcgtc gtcgaccagc aggcccagcg ccagcaccag cgagccgagc
     1381 gagatcttgt gcaggtcgat gccgaaatac ttcatcgcgg cgaaggtcat ggccagcacc
     1441 agcgggatgg acagcgccac caccatgccg ctgcgcgcac ccagcgagaa gaagctcacg
     1501 gcaagcacga tgaccaccgc ttcgaccagc gacttgacga actcgcccac cccgcggcgc
     1561 accgcgcgcg gctggtcgtt gacccggtcc agcgacagcc cgagcggcag gtgcgactgg
     1621 atgcgtgcga actcctggtc cagcgcctgc ccgaggcgga tgatgtcgcc gcccttgcgc
     1681 atcgacaccg cgatgcccag gcacggcacg ccgcggaagc gcatcagcgg gcccggcgga
     1741 tcgctgtagc cgcgcatcac ctcggccacg tcgccgacgc ggaaggtgcg gccgttgtac
     1801 gagatcggcg tgttgcggat gtcgtccacc gaccggtacg cgccggtggt gcgcaggtac
     1861 acgcggtcgt gatccagctc gaatgcgcca gccgggttga ccgagttctg ctgctgaatg
     1921 gcgtcgatga tcgcctgcgc cgggatgccg agctcggagc gcttggtgtt ggccaggcgc
     1981 acctcgatct tctcgtcctg cacgccgatg aactcgacct tggccacgtc cttcacccgc
     2041 agcagctggc cgcggatgtc gtcggcataa tccttcagct cggcgtagct gaagccgggc
     2101 ccggtcagcg cgtagatgtt gccaaaggtg tcgccgaact cgtcgttgaa gaacggcccg
     2161 agcacgccct gtggcaggtt ctgcctgatg tcgccgatct tcttgcgcac ctggtagtac
     2221 gcgtcctgcg tcgccgcgta gccgatgtcg tcgcgcacgc ggaagaacac ctgcgattcg
     2281 cccggccgcg aatagctggc gaggtagtcg acctgctcgt gttcctgcag gcgctcctcg
     2341 atgcggtcgg tgacctcctg gtccacctct gccgcggtgg cgcccggcca gtaggtgcgc
     2401 accaccatca gcgggaaggt gaacggcgga tcttccgact gtcccaggtg cgtgtatgac
     2461 cacgcgccga gcacgccgaa cagcaccatg aagtagacca ccagcccgcg gttggccagc
     2521 gcccactcgg aaagattgaa cgagcgcatc agccgttgtg ctggcgctgc agcaccgaca
     2581 cgtccatgcc ttcatgcatc aggtggatgc cggcggacac catgttcatc gccgcatcga
     2641 gcgcacccga caccaccgcg ccggtctcgg tgtagcgcag tacctgcacc ggcacctggt
     2701 gcgtcttgcc gtcgctgacg atccacaccg ccggctgctc cttgctgccg ccgagtgcgg
     2761 acatgggaat gatgaacgac cgcgcgtcga gcttctgacc gacgatcacc gtggcggtca
     2821 tgcccagctt caccgcgtcg tcggcatcgg ccatcgacac gcgcacttcg t
//
LOCUS       DASZZC010000488         4556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_678149,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000488 DASZZC010000000
VERSION     DASZZC010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678149"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..791)
                     /locus_tag="VHE37_10310"
     CDS             complement(126..791)
                     /locus_tag="VHE37_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT35970.1"
                     /translation="MTVVSATEVSKQVSSAGTALTIVDRVSLSIAAGESVAIVGASGS
                     GKTTLLSLLAGLDDASSGQIELLGQRLDTLDEDGRASLRAGRVGFVFQSFQLLAGFTA
                     LENVMLPAELAGLADARERAQAALAQVGLAARVSHYPSQLSGGEQQRVAIARAYAGAP
                     QVLFADEPTGNLDSATGARVIELLFELNRARNATLVLVTHDPALAQRCSRVLRLKDGR
                     IDA"
     gene            790..1437
                     /locus_tag="VHE37_10315"
     CDS             790..1437
                     /locus_tag="VHE37_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HVT35971.1"
                     /translation="MRVRLALVLCALLWSALGQAQEPKILLLGDSLGASYGVPVTQGW
                     VTLLQQRLMQKGYPHKIVNASVSGDTTAGGRTRLAEDIRTYQPQIVLIELGGNDGLRG
                     LPVAKMRENLEAMVQLCQEMEIRPVLFEMRIPDNYGKDYVSGFAGTFSSVAQETHTPL
                     VPFFLAGIAGDRAKWFQEDGIHPTAEAQPKLLDAVWPTLEKVLGRNPAHGGKKKK"
     gene            1622..2941
                     /locus_tag="VHE37_10320"
     CDS             1622..2941
                     /locus_tag="VHE37_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT35972.1"
                     /translation="MRIAVIGSGISGLGAAHYLAQRHDVVLYEQDARVGGHSNTVRAG
                     GHDIDTGFIVCNPVNYPNFYPFLDELGIARQDTDMSLGVSVEGGRIEWAGDENLAKIF
                     AQPALMFSPEHIRMLLAVLRFNKHVKQLLDADALPDITLGEFLERHAYPMSLRVRYVA
                     AMAGPIWSTSTRGVMDFPFPFFARFFDAHGLLNVYQRPQWQTVVGGSKNYVDAIVSRF
                     RGELRRGAAVRSLRRGGEAAWISTADGEDRYDAVVCAAHADQALRLLTDASEAERAVL
                     SQVPYTRNIAYLHTDETLMPRRRAAWSAWNALLARDALSDEPIGVTYWMNRLQNLPGR
                     EPYFVSLNPPQAPRAERVLYQTEYEHPLYNLGTLRAQELLPTMQGRNRVWWAGAWTRY
                     GFHEDGFTSALRAVAGIDRGCLPPWARLEPAHGAAPLPAPQLAPQAA"
     gene            2961..3716
                     /locus_tag="VHE37_10325"
     CDS             2961..3716
                     /locus_tag="VHE37_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HVT35973.1"
                     /translation="MDDGALYPARVMHRRRVAPLYRFHYDVFYLLVDIDRLDALHARL
                     KFFSHGRFNLLSLRDADHGDGRGLRAWAERMLSSAGIELAGGRIRLLAMPRVLGMAFN
                     PISLWYCEHADGSLRAVIAEVNNTFGEKHSYLLAADGAPLPWNAALDKDKCFHVSPFF
                     DLSGRYRFRLGEPREMLTVSIHQSRDDVSTMDATLTAMRMDLTDGAIIRQVLAMPWLM
                     FKVLAGIHWEALKLWLRGARFHRKPAPPAREIT"
     gene            3739..>4556
                     /locus_tag="VHE37_10330"
     CDS             3739..>4556
                     /locus_tag="VHE37_10330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT35974.1"
                     /translation="MEQDQDKFDRVMSRDGFSPDWPAQSPRAREGLLLRLFLRMLARL
                     RIGTLDVRLPSGELRSFRGSERQELHGVLQIRDERLMWQVLRNGEVGFGEAYVDGLWD
                     SPDLTRLLMLLYLNEPYLLGALQNNALSRLAGWVTHRLRANSRRQARRNIEYHYDLGN
                     EFYKLWLDETMTYSSALFEGPSQTLLDAQLNKFASMLRRLDLKPEHRLLEIGSGWGGF
                     AIYAAQNSGCRVHSITLSTEQLVEARRRAEAAGVADRVTFELRDYRDIEGQYDRV"
BASE COUNT          752 a         1529 c         1549 g          726 t
ORIGIN      
        1 cgaacagcga caccgacgaa gccgaggcca ccgcgatggt cagcgccagc gccaggatca
       61 gcagctcgcc cgagcgccag ccgcggcgca ggcggcgcag ggcaaaggtc agcgcgcgcg
      121 ccagctcacg catcgatccg cccgtccttc agtcgcagca cgcgcgaaca gcgctgcgcc
      181 agcgcgggat cgtgcgtcac cagcaccagc gtggcgttgc gcgcgcggtt gagctcgaac
      241 agcagctcga tgacgcgcgc gccggtggcg ctgtcgaggt tgccggtcgg ctcgtcggcg
      301 aacagcacct gcggcgcgcc cgcgtacgcg cgggcgatgg ccacgcgctg ctgctcgccg
      361 cctgacagct gcgacgggta gtgcgacacg cgcgcggcca gccccacctg cgccagcgcg
      421 gcctgcgcgc gctcgcgcgc atcggccaga ccggccagct ccgccggcag catcacattt
      481 tccagcgcgg tgaagcctgc caatagctgg aacgactgga acacgaagcc gacgcggccc
      541 gcgcgcagcg atgcgcggcc atcctcgtcc agcgtgtcga gacgctggcc gagcagttcg
      601 atctgcccgc tcgatgcatc gtccagcccg gccagcagcg acagcagcgt ggtcttgccc
      661 gaacccgacg cgccgacgat ggccacgctc tcgccggcgg cgatcgacag cgacacgcgg
      721 tcgacgatgg tcagcgccgt gccggcgctg ctaacctgct tgctcacttc tgttgcggat
      781 acgaccgtca tgcgtgtgcg gctcgctttg gtgttgtgtg cgctgctgtg gtccgcgctt
      841 ggacaggcgc aggagccgaa gattctgctg ctgggcgaca gtctcggcgc gtcctatggc
      901 gtgccggtga cgcagggctg ggtcacgctg ctgcagcagc ggctgatgca gaaaggctat
      961 ccgcacaaga tcgtcaacgc cagcgtgtcc ggcgacacca ccgccggcgg gcgcacgcgg
     1021 ctggcggagg atatccgcac ctaccagccg cagatcgtgc tgatcgagct cggtggcaac
     1081 gatggcctgc gcgggctgcc ggtggcgaag atgcgcgaga acctggaggc catggtgcag
     1141 ctgtgccagg agatggagat caggccggtg ctgttcgaga tgcgcatccc cgacaactac
     1201 ggcaaggatt acgtgagcgg cttcgccggc acgttttctt cggtggcgca ggaaacgcac
     1261 acgccgctgg tgccgttctt cctggccggc atcgccggcg accgcgcgaa atggttccag
     1321 gaagacggca tccatcccac cgcggaagcg cagccgaagc tgctcgacgc ggtgtggccc
     1381 acgctggaaa aagtcctcgg caggaatccg gcacacggcg gcaagaagaa aaagtgatgc
     1441 gcgttgcatc ctgttgcgcg gatgctgcgt ataacccccg gtacatcgga aaacatcgga
     1501 ctggaggcct taccgcactg ccgttttgtc tgttattaaa tattgtgtaa ttttaatttt
     1561 acatcataat ttataacagt agaatcggcg gtgcggtgag gctctgatcg ggggcttttg
     1621 catgaggatt gccgtcatcg gcagcggcat cagcgggctg ggcgcggcgc actacctggc
     1681 gcagcgtcac gacgtggtgc tgtacgagca ggatgcgcgc gtgggcgggc acagcaacac
     1741 cgtgcgcgcc ggcgggcacg acatcgacac cggcttcatc gtctgcaacc cggtcaacta
     1801 cccgaacttc tatccattcc tcgacgaact cggcatcgcg cggcaggaca ccgacatgtc
     1861 gctgggcgtg tcggtggagg gcgggcgcat cgagtgggcc ggagacgaga acctggccaa
     1921 gattttcgcc cagccggcgc tgatgttctc gcccgaacac atccgcatgc tgctggcggt
     1981 gctgcgcttc aataaacacg tgaagcagct gctcgatgcc gacgcgctgc ccgacatcac
     2041 gctcggcgaa ttcctcgagc ggcatgcgta cccgatgtcg ctgcgcgtgc gctatgtcgc
     2101 cgccatggcc gggcccatct ggagcacctc gacccgcggc gtgatggatt ttccgttccc
     2161 gttcttcgcg cgcttcttcg acgcgcacgg cctgctcaac gtgtaccagc gaccgcaatg
     2221 gcagacggtg gtgggcggct cgaagaatta cgtcgacgcc atcgtctcgc gtttccgcgg
     2281 agagctgcgc cgtggcgccg cggtgcgcag cctgcgccgc ggcggggagg cggcgtggat
     2341 cagcactgcc gacggcgagg accgctacga cgcggtggtc tgcgccgcgc acgccgacca
     2401 ggcgctgcgc ctgctcaccg acgccagcga ggcggagcgc gccgtgctga gccaggtgcc
     2461 gtacacgcgc aacatcgcct acctgcacac cgatgaaacc ctgatgccgc gccggcgcgc
     2521 ggcgtggagc gcgtggaatg cgctgctggc gcgcgatgcg ctgagcgacg agccgatcgg
     2581 cgtcacctac tggatgaacc gcctgcagaa cctgccgggg cgcgagccgt atttcgtctc
     2641 gctcaatccg ccgcaggcac cgcgcgcgga acgcgtgctg taccagaccg agtacgaaca
     2701 tccgctgtac aacctcggca cgctgcgcgc gcaggaactg ctgcccacga tgcagggtcg
     2761 caaccgcgtg tggtgggccg gcgcgtggac gcgttatggc ttccacgagg acggcttcac
     2821 ctcggccctg cgtgcggtgg ccggcatcga ccgcggctgc ctgccgccgt gggcgcggct
     2881 ggagccggcg cacggcgccg cgccgctgcc cgcgccgcag ctggcgccgc aggcggcatg
     2941 agcccggagc agatcgcccc atggacgatg gcgcgctcta tcccgcccgc gtgatgcacc
     3001 ggcgccgcgt ggcgccgctg taccgcttcc actacgacgt gttctacctg ctggtcgaca
     3061 tcgaccggct cgatgcgctg cacgcgcggc tgaagttttt ctcgcacggg cgcttcaacc
     3121 tgctgtcgct gcgcgatgcc gatcatggcg acggccgcgg cctgcgcgcc tgggccgagc
     3181 gcatgctgtc ctccgccggc atcgagctgg ccggcgggcg catccgcctg ctggccatgc
     3241 cgcgcgtgct gggcatggcg ttcaacccga tcagcctgtg gtactgcgag cacgcagacg
     3301 ggagtttgcg cgcggtaatc gcggaggtga acaacacctt cggcgaaaaa cacagctacc
     3361 tgctggccgc cgacggtgcg ccgctgccgt ggaacgcggc gctggacaag gacaagtgct
     3421 tccacgtctc gccgttcttc gacctgtccg gccgctaccg cttccggctg ggcgagccgc
     3481 gcgaaatgct cacggtaagc atccaccagt cgcgcgacga cgtatccacc atggacgcga
     3541 cgctgaccgc gatgcggatg gacttgaccg atggagccat catccgccag gtgctggcca
     3601 tgccctggct gatgttcaag gtgctggccg gcatccattg ggaagcgctg aaattgtggc
     3661 tgcgcggcgc gcgcttccac cgcaagccgg caccgccggc gcgcgaaatc acctaagcaa
     3721 gaactagagc agaacaagat ggaacaggac caggacaagt tcgaccgtgt gatgagtcgc
     3781 gatggttttt cgcctgactg gccggcgcag tcgccgcgtg cgcgcgaggg cctgctgctg
     3841 cgcctgttcc tgcgcatgct ggcgcgcctg cgcatcggca cgctcgacgt gcgcctgccc
     3901 tcgggcgagc tgcgcagctt ccgcggcagc gaacggcagg agctgcacgg cgtgctgcag
     3961 atccgcgacg agcgcctgat gtggcaggtg ctgcgcaacg gcgaggtcgg ttttggcgaa
     4021 gcgtatgtcg acggcctgtg ggattcgccc gatctcacgc gcctgctgat gctgctgtat
     4081 ctcaacgagc cctacctgct cggcgcgctg cagaacaatg cgctcagccg cctggccggc
     4141 tgggtcactc accggctgcg ggccaacagc cgccgccagg cgcggcgcaa catcgagtac
     4201 cactacgacc tcggcaacga gttctacaag ctgtggctcg acgagaccat gacgtattcg
     4261 agcgcgctgt ttgaagggcc gagccagacg ctgctcgatg cgcagctgaa caagttcgcc
     4321 agcatgctgc ggcggctcga tctcaaaccc gaacaccggt tgttggaaat cggctcgggc
     4381 tggggaggct ttgccatcta cgccgcgcag aacagcggct gccgcgtgca cagcatcacg
     4441 ctgtcgaccg agcagctggt ggaagcgcgc cggcgcgccg aggcggcggg cgtggccgac
     4501 cgggtgacct tcgagctgcg cgactaccgc gacatcgaag gccagtacga tcgcgt
//
LOCUS       DASZZC010000489         8476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_491258,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000489 DASZZC010000000
VERSION     DASZZC010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491258"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHE37_10335"
     CDS             complement(<1..183)
                     /locus_tag="VHE37_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35975.1"
                     /translation="MERVRRRWWTLSISLLAVLIILAALISGLFQLAVLALPDYRDDL
                     SVWVTKVAGRPVQIGGV"
     gene            complement(424..1890)
                     /gene="rng"
                     /locus_tag="VHE37_10340"
     CDS             complement(424..1890)
                     /gene="rng"
                     /locus_tag="VHE37_10340"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HVT35976.1"
                     /translation="MSTEILVNIGAHETRVALVEGGAAQEVYVQRASRHGLTGNIYKG
                     LVQRVLPGMQAAFIEIGLERTAFLHVSDMLPPPSNGDAPAPPPQITRLLHEGQEVLVQ
                     VLKDPLGSKGARLTTLLSIPSRYLVLLPHEKNVGVSARIEDEAERTRLKDHLTVIQAA
                     LAPAFGIIARTAADGADAQALENDVKFLLRLWAGVSEQARSSRPGTLVHGDLPLSMRI
                     LRDLLGTDVERVRVDHAGACRDLRQFAAVFVPQVADKIEHFAGAAPIFDLYGVEDDIN
                     RALQRRVDLKSGGHLVFDQTEAMTTVDVNTGAYTGHKNLEETVLKTNLEAAQAIARQL
                     RLRNLGGIIVLDFIDMKSEEHREQVLRAFERALASDYARTQVYPFSPLGLLEMTRKRS
                     RESLGHILCEPCPACEGRGSVKTVETVCHEIAREVQRAARQFEAKGFLVLASPRVTQY
                     LMEDQPAQLAELEAQLNRPIRLQAEAAYGQENFDVVPL"
     gene            complement(1967..2551)
                     /locus_tag="VHE37_10345"
     CDS             complement(1967..2551)
                     /locus_tag="VHE37_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HVT35977.1"
                     /translation="MSAIDLYLASQSPRRAELLMQLGLRFEIVPAEVVEQPRPGETPA
                     QYALRIAQDKARAGATHARVALPVLGADTDVVLDGKVLGKPRDAAHAIDMLLALSGRM
                     HEVYSAVALMLGERDETALSVTRVEFGDITRAQAQAYWDSGEPRGKAGAYAIQGLGAR
                     FVRRIEGSYSGVVGLPLYETMELLQRLQDHNKKP"
     gene            complement(2685..3155)
                     /gene="rlmH"
                     /locus_tag="VHE37_10350"
     CDS             complement(2685..3155)
                     /gene="rlmH"
                     /locus_tag="VHE37_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HVT35978.1"
                     /translation="MNIVLAAVGHKMPAWVQAAYADYAARLPRECTLLLREIAPAQRG
                     KNADLARAKKEEGEKLLRAIPRDALVIALDERGRELTSTQWSRELAGWMQSGRDVALL
                     IGGADGHASEVLAHAQQSWSLSKLTLPHAMVRVLVAEQLYRAWTLLANHPYHRA"
     gene            complement(3152..3805)
                     /locus_tag="VHE37_10355"
     CDS             complement(3152..3805)
                     /locus_tag="VHE37_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVT35979.1"
                     /translation="MEPSSRYIPALRFAALTRIYDPVVALTTRERTFKQRLIEQAHLA
                     PGLRVLDLACGSGTLAVWMKRAQPAIELTAIDGDPAILAQARAKAARLGLPIDFRQGL
                     STALPFENASFERVTSTLFFHHLQSPDKLKSFSEALRVLAPGGELHVADWGAPSNPAM
                     RAAFMGIRLLDGFANTAAHVAGRLPHMMREAGFDDVRVHGGIDTLFGTMALYSGRKA"
     gene            complement(3921..4379)
                     /gene="rsfS"
                     /locus_tag="VHE37_10360"
     CDS             complement(3921..4379)
                     /gene="rsfS"
                     /locus_tag="VHE37_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HVT35980.1"
                     /translation="MTQLAISALEDLKAQDLKVLDVRSQTTLTDTMVVCTGTSSRHVK
                     ALAGNLVTSAKHAGFQPGGVEGMEVGEWVLVNLGEVVVHIMQVQTRAFFQLEKLWEMQ
                     PPPEAAITEPKPKKAKSARKAAAKKSAAKPASKAAQRKGAAKKAPRSRRS"
     gene            complement(4390..5055)
                     /gene="nadD"
                     /locus_tag="VHE37_10365"
     CDS             complement(4390..5055)
                     /gene="nadD"
                     /locus_tag="VHE37_10365"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HVT35981.1"
                     /translation="MTQAIGIFGGAFSPFHNGHLRLAIEARDRLELGAVHLVPTAYPP
                     HRPDSRISPHKRFEWVRLAARREPGLVADDCEIRREGPSYTVDTLRGMRERYMATPLV
                     LLMGADAFGHLHSWSRWREILTLAHIAVVTRPGASVEASHEAAAELAGRRIEDFRVLH
                     EEPAGRWLTIDPPVLDISSTRIRCLLREGRSVRGLVPDAILNSMSASDRAALAQDEDA
                     TTH"
     gene            complement(5196..6485)
                     /locus_tag="VHE37_10370"
     CDS             complement(5196..6485)
                     /locus_tag="VHE37_10370"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HVT35982.1"
                     /translation="MSPTPAKPAKKSDAIATYMRERGAKARAAARELARADTGIKNAC
                     LFALAQLLLADSARILEANAQDMREGRAAKLDDAMLDRLELNEDRVAAMADGVKQVAA
                     LSDPVGELTDLSTRPSGIQVGRMRVPLGVVGIIYESRPNVTADAAALCIKSGNAAILR
                     GGSEALQSNQAIARCIERALTDAGLSKDTVQVLDITDRRTVSEMVALPEFIDVVIPRG
                     GKGLVAAVSENARVPVIKHLDGICHVYIDGMADIEKAIAIAVNAKTQRYGTCNTMETL
                     LVDKDIARNVLPRLGQIYGEAGVELRGCERTRKILPEIKPATEADWHTEYLAPILSIR
                     TVDGIDGAIDHIAKYGSAHTDSIVTESYSRVRRFLREVDSSSVMVNASTRFADGYEYG
                     LGAEIGISTDKFHARGPVGLEGLTSQKWIVLGDGHVR"
     gene            complement(6482..7507)
                     /gene="holA"
                     /locus_tag="VHE37_10375"
     CDS             complement(6482..7507)
                     /gene="holA"
                     /locus_tag="VHE37_10375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HVT35983.1"
                     /translation="MRLTSASLSAHLAKGLAPLYLVGGEEPLLVAESLDAIRAVARKA
                     GYAEREVLDADKSFNWQKVIDACASMSLFASLRLVEISLPSGAPGVEGSKVLQQLAAK
                     PPQDVLLILICGAVDWRSRQGSWYGAWESNGAALYFEPMKPAEQGAWLSARLKGAGLS
                     LDEEAVALLAQRTEGNLLAAAQDVEKLKLLFPGGKISAAELEQAVADSARYEAFDFIG
                     LMQEGDGAATVRALGRLREEGYEVPEILGALAWSLRQWAQVQAAFAQTRDAGRACEMA
                     HVPRRSQPQLARAVTRTRLAQVYGWIRKCAAIDQLAKSTGGKDQAWEELLTLALAASG
                     ISSPARA"
     gene            7653..>8476
                     /locus_tag="VHE37_10380"
     CDS             7653..>8476
                     /locus_tag="VHE37_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35984.1"
                     /translation="MWKRAWLLLSGCAVLLIAASCATPYKSYGLAGGYSDRRINDSAY
                     FVAFSGNGYASKEHVWNMWMYRCAELTLEKGYAYFFIKPGDPPATVSRAAGGLLPAVY
                     HPRHPLRDALARLHSAPARLHKTYYTYYSTTITTWNSRGTVLMFHQPLPAEMLWALDA
                     QTVINDLAAYVQNNGSAPAPTREQIYDHAFVAHARFDYGDDTLRVIPAPEAGASATAP
                     RSPAAIRSIIGAARVGFLAAFREHQRSDPAGGSVTIGFTVNSGGIVTDSHVVSSTM"
BASE COUNT         1325 a         2896 c         2811 g         1444 t
ORIGIN      
        1 gacgccgccg atctgcaccg ggcgcccggc gaccttggtc acccacaccg aaagatcgtc
       61 gcggtaatcg ggcagcgcca gcaccgccaa ctggaacagc ccgctgatca gcgccgccag
      121 gatgatcagc accgcgagca gcgagatgct cagcgtccac cagcggcgtc ttacccgttc
      181 catggcgcgc gcagagaagg tgaaatcagc agaagggaca tggacggcag gcgccgcggc
      241 gcggcgctac ggaaaatcct gtgatcgaaa aaccggcgtg cgggacagaa aattccggca
      301 accgcacctg ctcctggggt gttatttatt attgaacgaa tttaaaagcg ttaaataaaa
      361 tgtaaacgcc gcaagagcag tgcggtgcat tgtaggacag cagccgcgcc gacgtttcgc
      421 gcgctacagc ggcaccacgt cgaagttctc ctgcccatag gccgcttccg cctgcaaccg
      481 gatgggacgg ttcagctgcg cttcgagttc cgccagctgc gccggctggt cttccatcag
      541 gtattgggtg acgcgcggcg aggccagcac caggaaaccc ttggcctcga actggcgcgc
      601 ggcgcgctgc acctcgcgcg cgatctcatg gcacaccgtc tccaccgtct ttacgctgcc
      661 gcggccctcg caggccgggc acggctcgca caggatatgg ccgagcgatt cgcgcgaacg
      721 cttgcgcgtc atctccagca ggcccagcgg cgaaaacggg tacacctgcg tgcgcgcgta
      781 gtcggaggcc agcgcgcgct cgaaggcgcg cagcacctgc tcgcggtgct cctccgattt
      841 catgtcgatg aagtccagca cgatgatgcc gccgaggttg cgcaggcgca gctgccgcgc
      901 aatggcctgc gccgcctcga ggttggtctt gagcaccgtc tcttccaggt tcttgtgccc
      961 ggtgtaggcg ccggtgttga cgtccaccgt ggtcatcgcc tcggtctggt cgaacaccag
     1021 gtggccgccg gacttcagat ccacgcgccg ctgcagcgcg cgattgatgt cgtcctccac
     1081 gccgtacagg tcgaagatcg gcgccgcgcc ggcgaagtgc tcgatcttgt ccgccacctg
     1141 cggcacgaac accgcggcga actggcgcag gtcgcggcag gcgccggcgt gatccacgcg
     1201 cacacgctcg acgtcggtgc cgagcaggtc gcgcaggatg cgcatcgaca gcggcaggtc
     1261 gccgtgcacc agcgtgccgg gccggctgct gcgcgcctgc tcggacacgc cagcccacag
     1321 gcgcagcagg aatttcacgt cgttctccag cgcctgcgca tcggcgccgt cggccgcggt
     1381 gcgtgcaatg atgccgaagg ccggcgccag cgccgcctgg atcacggtga ggtgatcctt
     1441 cagccgcgtg cgctcggcct cgtcctcgat gcgcgccgac acgcccacgt tcttctcgtg
     1501 cggcagcagc accaggtagc gcgagggaat ggacagcagc gtggtcaggc gcgcgccttt
     1561 cgatcccaac ggatccttca gcacctgcac cagcacttcc tgcccttcgt gcagcaggcg
     1621 ggtgatctgc ggcggcggcg cgggcgcatc gccgttggat ggcggcggca gcatgtcgga
     1681 cacgtgcagg aacgcggtgc gctcgagccc gatctcgatg aacgccgcct gcatgcccgg
     1741 cagcacgcgc tgcaccaggc ccttgtagat gttgccggtg aggccgtggc gcgaggcgcg
     1801 ctgcacgtac acctcctgcg cggcgccgcc ttccaccagg gcgacgcgcg tttcgtgcgc
     1861 gccgatgttg accaatattt cggtactcat ggagcgccgc cttccaccaa ggcaagcgac
     1921 cattcgggcg tttcgtgcgc accgatgttg accaggattt ccgtgctcat ggttttttgt
     1981 tgtggtcctg cagccgttgc agcaactcca tcgtctcata cagcggcagc ccgaccacgc
     2041 cgctgtagct gccctcgatg cgccgcacga agcgcgcacc caggccctgg atggcatacg
     2101 cgccggcctt gccgcgcggc tcgccgctgt cccagtaggc ctgcgcctgc gcgcgggtga
     2161 tgtcaccgaa ttccacacgt gtcaccgaca atgcggtctc gtcgcgttcg ccgagcatca
     2221 gcgccaccgc gctgtagacc tcgtgcatgc ggccggacag cgccagcagc atgtcgatgg
     2281 catgcgccgc gtcgcgcggc ttgcccagga cttttccgtc gagcaccacg tcggtgtcgg
     2341 cgcccagcac cggcaacgcc acgcgcgcat gcgtcgcgcc tgcccgcgcc ttgtcctgcg
     2401 cgatgcgtag cgcgtactgc gcgggcgtct cgcccgggcg cggctgctcc accacttccg
     2461 ccggcacgat ctcgaagcgc aggcccagct gcatcagcag ctcggcgcgg cgtggggatt
     2521 gcgaggccag gtagaggtcg atggcggaca tggggtggtg attatcgcaa agcgtctgcg
     2581 cgcgggacgc ccgctagcgg agctcggtgc gccggcgtcg ggaatttcat catcgattca
     2641 cgcaacacgc gcgcatgaag ctcacagctc agcgcgctgc aagctcacgc gcggtgatat
     2701 ggatgattcg ccagcagagt ccacgcccgg tacagctgct ccgccaccag cacccgcacc
     2761 atcgcatgcg gcagcgtcag cttcgacagc gaccagctct gctgcgcgtg cgccagcacc
     2821 tcggacgcgt ggccgtcggc gccgccgatc aacagcgcca cgtcgcggcc cgactgcatc
     2881 cagccggcca gctcgcgcga ccactgcgtg ctggtcagtt cgcggccgcg ctcgtcgagc
     2941 gcgatgacca gcgcatcgcg cgggatcgcg cgcagcagct tttcgccctc ttcctttttc
     3001 gcgcgcgcca ggtccgcgtt cttgccgcgc tgcgccggcg cgatttccct gagcagcagc
     3061 gtgcattcgc gcggcaggcg cgcagcgtag tcggcatagg cggcctgcac ccacgccggc
     3121 atcttgtggc cgacggcggc gagcacgatg ttcacgcttt ccgtccgctg tagagcgcca
     3181 tggtgccgaa cagcgtgtcg atgccgccat gcacgcgcac gtcgtcgaag ccggcctcgc
     3241 gcatcatgtg cggcaggcgg ccggccacgt gcgcggcggt gttggcgaaa ccgtcgagca
     3301 ggcggatgcc catgaacgcg gcgcgcatcg ccggattgct cggcgcgccc cagtcggcca
     3361 cgtgcagttc gccgccggga gcgagcacgc gcagcgcctc ggaaaaactt ttcagcttgt
     3421 cgggcgactg cagatgatgg aagaacaagg tggaggtcac gcgctcgaag ctggcgttct
     3481 cgaacggcaa cgcggtggac aggccttggc ggaaatcgat cggcaggccg agccgagccg
     3541 ccttggcgcg cgcctgcgcg aggatcgcgg gatcgccgtc gatggcggtg agctcgatgg
     3601 ctggctgcgc gcgcttcatc cacaccgcca gcgtgccgct gccgcaggcc aggtcgagca
     3661 cgcgcaggcc gggcgccagg tgggcctgtt cgatcagcct ctgcttgaag gtgcgttcgc
     3721 gcgtggtcag cgcgaccacc gggtcgtaga tgcgcgtcag cgcggcaaag cgcaaggcgg
     3781 ggatatagcg gctcgatggc tccatgcgtg ctgtttttcc cttcaccgca ccccgcatct
     3841 tggcgttatt aaatatcgtc tattttaatt tttagacaat aataaataac acgtggaatg
     3901 acgatgcggt ggacgtattt tcagctccgc cgcgagcgcg gagccttctt cgcggcgcct
     3961 ttgcgctgag ccgctttcga tgccggcttc gccgcggatt tttttgctgc cgctttgcgt
     4021 gcgctcttcg ccttcttcgg tttgggctcg gtgatggccg cttccggcgg cggctgcatc
     4081 tcccacagtt tctccagctg gaagaaggcg cgcgtctgca cctgcatgat gtgcaccacc
     4141 acttcaccca ggttcaccag cacccactcg cccacttcca tgccttcgac accgccgggc
     4201 tggaagccgg catgcttggc actggtgacc aggttaccgg ccagcgcctt cacgtggcgg
     4261 ctggacgtgc cggtgcacac caccatggtg tcggtcagcg tggtctggct gcgcacgtcg
     4321 agcactttca gatcctgcgc cttgagatcc tccagcgcgc tgatcgccag ctgcgtcacc
     4381 ggcgatttcc tagtgggtcg tggcatcttc atcctgggct agcgccgcgc ggtcggacgc
     4441 ggacatggaa ttgagtatcg catccggcac cagtccgcgc accgaacggc cctcgcgcag
     4501 caggcagcgg atgcgcgtgg aggaaatgtc cagcaccggt ggatcgatgg tcagccagcg
     4561 gccggccggc tcctcgtgca gcacgcggaa atcctcgatg cggcggccgg caagttcggc
     4621 agcggcctcg tgcgacgcct ccacgctggc gccgggccgc gtcaccaccg cgatatgcgc
     4681 cagcgtgaga atctcgcgcc agcggctcca gctgtgcagg tggccgaagg catcggcgcc
     4741 catcagcagc accagcggcg tggccatgta gcgctcgcgc atgccgcgca gcgtgtctac
     4801 cgtgtacgac ggtccttcgc ggcggatctc gcagtcgtcg gccaccaggc ccggctcgcg
     4861 ccgcgccgcc agccgcaccc actcgaagcg cttgtgcggc gagatgcgcg aatccggccg
     4921 gtgcggcgga taggccgtgg gcaccaggtg caccgcgccc agttcgaggc ggtcgcgcgc
     4981 ctcgatggcc agccgcaggt ggccgttgtg gaacggcgag aacgcgccgc cgaagatgcc
     5041 gatggcctgc gtcactaata tttccttcga tgtaaaacgc gtcgcgagcg aaggcaggac
     5101 gaggcgcgag cagccgcgaa gccggagtta acagtgaagt taatgaggat ttcgcggctg
     5161 cgaggaaacg aagtcatgcc gggcacagca gcgttttacc gcacatggcc gtcgccgagg
     5221 actatccact tctgcgaggt gagcccttca aggcccaccg gcccgcgcgc gtggaacttg
     5281 tcggtggaga tgccgatctc cgcgcccagg ccgtattcgt agccgtcggc aaaacgcgtg
     5341 gaggcgttga ccatcaccga actggagtcg acctcgcgca ggaagcggcg cacgcgcgag
     5401 tagctctcgg tgacgatgga atcggtgtgc gccgaaccgt acttcgcgat gtggtcgatg
     5461 gcgccgtcga tgccgtcgac ggtgcggatc gacaggatcg gcgccaggta ctcggtgtgc
     5521 cagtcggcct cggtggccgg cttgatctcc ggcaggatct tgcgcgtgcg ctcgcagccg
     5581 cgcagttcca cgccggcttc gccgtagatc tggcccaggc gcggcagcac gttccgggcg
     5641 atgtccttgt cgaccagcag cgtctccatg gtgttgcagg tgccgtagcg ctgggtcttg
     5701 gcattgaccg cgatggcgat ggctttctcg atgtcggcca tgccgtcgat atacacgtgg
     5761 cagatgccgt cgagatgctt gatcaccggc acgcgcgcgt tctcgctcac cgcggccacc
     5821 aggcccttgc cgccgcgcgg gatgaccacg tcgatgaatt ccggcagcgc caccatctcg
     5881 ctcaccgtgc ggcggtcggt gatgtcgagc acctgcaccg tgtccttgga caggcccgcg
     5941 tcggtcagcg cgcgctcgat gcagcgcgcg atggcctgat tgctctgcaa tgcttccgaa
     6001 cctccgcgca ggatggcggc gttgcccgac ttgatgcaca acgcggccgc gtcggcggtc
     6061 acattcgggc gcgattcata gatgatgccg accacgccca gcggcacgcg catgcggccc
     6121 acctggatgc cggacggacg cgtcgacagg tcggtgagtt cgccgaccgg gtcggacagc
     6181 gcggccacct gcttcacgcc gtcggccatc gcggcgacgc ggtcctcgtt gagttcgagg
     6241 cggtcgagca tggcgtcgtc gagcttggcc gcgcggcctt cgcgcatgtc ctgagcgttg
     6301 gcttcgagga tgcgcgcgct gtccgccagc agcagttgcg ccagcgcaaa caggcaggcg
     6361 ttcttgatgc cggtgtcggc gcgtgccagt tcgcgcgcgg ccgcgcgcgc cttggcgcca
     6421 cgctcgcgca tgtaggtggc gatggcgtcg gacttcttgg caggcttggc gggggtggga
     6481 ctcatgctct cgctggggag gaaatgccgc tggcggcgag cgccaatgtt agcaactctt
     6541 cccaggcctg gtccttgccg ccggtggact tggccagctg gtcaatcgcg gcgcatttgc
     6601 gtatccatcc atacacttgt gcgagccgcg tgcgcgtcac cgcacgcgcc agctgcggct
     6661 ggctgcgacg cggcacgtgc gccatctcgc aggcgcggcc ggcgtcgcgc gtctgcgcaa
     6721 atgcagcctg cacctgcgcc cactggcgca ggctccaggc caatgcgccg aggatctccg
     6781 gcacttcgta gccttcctcg cgcaggcggc cgagcgcgcg cacagtggcg gcgccgtcgc
     6841 cttcctgcat cagtccgatg aagtcgaagg cctcgtagcg agcgctgtcg gccacggcct
     6901 gctccagctc ggcggcgctg atcttgccgc cgggaaagag cagcttgagt ttttccacgt
     6961 cctgcgcggc cgcgagcagg ttgccctcgg tgcgctgggc cagcagcgcg accgcttcct
     7021 cgtccagcga gagtcccgca cccttgaggc gggcagagag ccaggcgcct tgttcagccg
     7081 gcttcatcgg ctcgaagtac agcgccgccc cgttgctctc ccaggcgcca taccagctgc
     7141 cctggcggct gcgccagtcg accgcgccgc agataaggat caacagcacg tcctgcggcg
     7201 gcttggccgc cagctgctgc agcaccttgc tgccttccac gcccggcgcg ccggacggca
     7261 ggctgatctc caccagccgc aggctggcga acaaggacat cgatgcgcag gcatcgatca
     7321 ccttctgcca gttgaagctc ttgtcggcat ccagcacttc gcgctcggca tagccggcct
     7381 tgcgtgcgac ggcgcggatg gcgtcgagcg actcggccac cagcaagggt tcttcaccgc
     7441 cgaccaggta cagcggcgcc aggcccttgg ccaggtgagc ggacagcgaa gcggaagtga
     7501 ggcgcactaa ggtaagtccc ggcaatcgga agacgctcat tttatgggct ggcgcgaacc
     7561 ggggttttcc tcatttacct gcgccaccgc cgcggttagc ctgaggacgg ggtgaggccc
     7621 gcaagggcgt cagttttttg ggggaacgga acatgtggaa acgcgcctgg ctgctgctgt
     7681 cgggatgtgc ggtgctgctg atcgccgcaa gctgcgcaac gccgtacaag tcttacgggc
     7741 tggccggcgg ttacagcgac cgccgcatca atgactcggc ttacttcgtc gccttcagcg
     7801 gcaacggcta cgcgtcgaag gaacacgtgt ggaacatgtg gatgtaccgc tgcgccgagc
     7861 tcaccctgga aaaaggctac gcgtacttct tcatcaagcc cggcgatccg ccggccacgg
     7921 tttcgcgcgc cgccggcggc ctgctgcccg ccgtgtatca cccgcggcat ccgctgcggg
     7981 atgctctggc ccggctgcat tccgcgcctg cgcggctgca caagacctac tacacctatt
     8041 actcgacgac catcaccacc tggaattcgc gcggcaccgt gctgatgttt caccagcccc
     8101 tgccggccga aatgctgtgg gcgctggatg cgcagaccgt gatcaacgac ctggcggcct
     8161 acgtccagaa caacggctcg gcgccggcgc cgacccgcga gcagatctac gaccacgcgt
     8221 tcgtcgcgca cgcgcgcttc gactacggcg acgacacgct gcgtgtcatt ccggcccccg
     8281 aggcgggcgc gtcggcgacg gcgccccggt cgccggcggc catccgcagc atcatcggcg
     8341 cggcgcgagt cgggttcctg gcggcgttcc gcgaacacca gcgcagcgat cctgcgggcg
     8401 gcagcgtcac catcggcttc accgtcaatt ccggcggcat cgtcaccgac agccacgtgg
     8461 tgtcgagcac catgca
//
LOCUS       DASZZC010000490         6522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_361826,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000490 DASZZC010000000
VERSION     DASZZC010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361826"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1056
                     /locus_tag="VHE37_10385"
     CDS             133..1056
                     /locus_tag="VHE37_10385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT35985.1"
                     /translation="MPFALKAVGAVAGIMLIAPIAGAAENPVPRSAADIIAASKPADW
                     RALDPQHTLYLELASGRVVIELAPAFAPAHVANIEALAREHYFDGLAIVRVQDNYVVQ
                     WGDPAGDDAQRKPFKNAKKELQPEYSVPYSDALPFARLADGDLYAPQVGFSYSLPAAR
                     DPKTQQTWLTHCYGMVGVGRGDNPDNGNGSELYVVIGNAPRHLDRNVIVVGRVMQGIE
                     RLSSLPRGTGNLGFYEKPEQYVPIRSVRLASDVPPAERSELEVLRSDTPTFAAFVDAR
                     RNRREAWFQDPVGHIELCNVPVVVRAPAAGR"
     gene            1280..1819
                     /locus_tag="VHE37_10390"
     CDS             1280..1819
                     /locus_tag="VHE37_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35986.1"
                     /translation="MNQAQASIDATQELPAASSEPLYFAVSTRKLAVMSVVTLGLYEV
                     WWFSWNWRLIKQRERSDIMPVGRAIFALFFVYDLFKRICASAEEHGAARTSAGALASG
                     WIVLSLTWRLPDPYWIISMFAFVCLLPMQKLVNQINQRVAPAHEPNDRFSGWNIFGIV
                     VGGIMMLLAIVGAFLPDPG"
     gene            complement(1849..3408)
                     /gene="hutH"
                     /locus_tag="VHE37_10395"
     CDS             complement(1849..3408)
                     /gene="hutH"
                     /locus_tag="VHE37_10395"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVT35987.1"
                     /translation="MKDIELDGASLSFAQVRAVAYGKPGHPRVTLARTARKRVARAAA
                     GVQRLLKEGRIAYGITTGFGAFKDKVISPADVETLQRNIIVSHAVGVGRHYDIPTTRA
                     IMLIRANTLSRGYSGVRPSTLELLLELLNRGVHPLIPEKGSLGASGDLAPLAHMSLTL
                     IGEGEAEYFGQTFSSAEALALAKLQPLTLAAKEGLALTNGTSVMCALGVLETLRAERM
                     SQTADIAGCLSLEALNGTDLAFDPRIHALRAMPRQVECAAYLRRLLAGSRLTRAHQSN
                     DVQDAYTLRCIPQVHGAVRDAIAYARWIFEMELNAVTDNPLLFVEGKKVEVLSGGNFH
                     GEPLAIAMDYLGIALAELGNISERRTMRLTDEASNKHVLPAFLTEQGGLNSGFMIVQY
                     TAAALATENKVLAHPASVDTIPTSANVEDHVSMGVTAGLKLRAIADNVERILAIELLS
                     AAQGIDFRHRALRRRATLGRGTRAAYALIRSRVPSIERDAVMYPHMETVSALVRDGSV
                     VEQVNRALARG"
     gene            complement(3419..4690)
                     /gene="hutI"
                     /locus_tag="VHE37_10400"
     CDS             complement(3419..4690)
                     /gene="hutI"
                     /locus_tag="VHE37_10400"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HVT35988.1"
                     /translation="MQAADLLIYNAAQLLTCAWPTAKRGAAMTDVGLIEDAAIAIGGE
                     KIIQIGKSAELCRHFKADNEIDARGKVICPGLVDPHTHVVFAGDRIGEFERRIRGESY
                     QDIAASGGGIVSTVSATRAATLAQIVIESSRRLDRMLALGTTTAEAKTGYGLDADSEL
                     KLLLATEALDKNHAIDLVPTFLGAHAVPPEFRGRGDDYIDLLLAEMLPAAAEWYRQSR
                     FAAQRVPMFVDVFCEQHAFTLAQSRRVLEAAQALGLPLKAHVDEFSQLGALDMALGLG
                     AVSVDHLDETDGDGIARLAASNSIGVIIPTVNFHLGATRYANARAMLDAGCALALTTD
                     INPGSAPCPSLPMAMAIACRYQKLAPAEALNACTINAAYAVGLGERTGSLQAGRQADL
                     ILLDATDWRVLAYEFGGNAVARVVKRGRIVV"
     gene            complement(4700..6346)
                     /gene="hutU"
                     /locus_tag="VHE37_10405"
     CDS             complement(4700..6346)
                     /gene="hutU"
                     /locus_tag="VHE37_10405"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVT35989.1"
                     /translation="MKREIHAPTGSTLSCRNWLIEAAYRMIQNNLDPQVAFDSDNLIV
                     YGGRGRAARNWDCYDAILASLKKLEPDETLLVQSGKPVAVFRTHEDAPRVLLANSNIV
                     PHWATQQNFDRWEHEGLIMFGQMTAGSWIYIGTQGILQGTYETFGALARQHGWGTLKG
                     KLVLTAGLGEMGGAQPLAITMNEGVGLLVEVDPWRVQRRLELKQLDVGTASLDEALAW
                     ARDACERGIAKSIGLIGNAADVLPEILKRGVKPDVVTDQTSAHDVLYGYIPAGLSLGD
                     AENLRKSDQAEYERRAMDSMAAHVRAMLSFQKQGAVVFDYGNNLRQRALDHGVKHAFD
                     YPGFVPAYIRPLFCEGKGPFRWVALSGDAEDIYRTDRAVMELFPGNAHLHRWLKMARE
                     QVKFQGLPARICWLGYGERAKAGLKFNEMVASGELKAPVVIGRDHLDSGSVASPNRET
                     EAMKDGSDAIADWPLLNAMINAVGGATWVSIHHGGGVGIGFSIHAGQVIVADGTPAAA
                     RRLQRVLTSDPGMGVARHADAGYDQAREFARTHGVKIPMP"
BASE COUNT         1013 a         2159 c         2211 g         1139 t
ORIGIN      
        1 ggagccggcg gtgtactacg acagctacac cggcgtggcg gaattttcct attcgcccga
       61 ctggccgccc aacacgcgct accgctgcac cgccaagctc gacgagcgcg actgggtgtg
      121 caagccgccg tgatgccctt cgcactgaaa gccgtcggcg cagttgccgg catcatgctg
      181 atcgcgccca tcgccggagc tgccgaaaac ccggttccgc gcagcgccgc cgacatcatc
      241 gcggcgtcga agcccgccga ctggcgagcg ctcgacccgc agcacacgct ctatctcgaa
      301 ctggcctcgg gccgcgtggt gatcgagctg gcgcccgcct tcgcgccggc ccacgtggcc
      361 aacatcgaag cgctggcgcg cgagcattat ttcgacggcc tggccatcgt gcgcgtgcag
      421 gacaactacg tggtgcagtg gggcgatccc gccggggacg acgcgcagcg caagccgttc
      481 aagaatgcga agaaggaact gcagccggaa tactcggtgc cgtacagcga cgcgctgccg
      541 ttcgcgcgcc tggccgacgg cgacctgtac gcgccgcagg tcggcttctc ctacagcctg
      601 ccggcggcgc gcgatccgaa aacgcagcag acctggctga cgcattgcta cggcatggtc
      661 ggcgtcggcc gcggcgataa cccggacaac ggcaacggca gcgaactcta cgtggtgatc
      721 ggcaatgcgc cgcgccatct cgaccgcaac gtcatcgtgg tcgggcgcgt gatgcagggc
      781 atcgagcggc tgtcgagcct gccgcgcggt accggcaacc tgggctttta cgagaagccc
      841 gagcagtacg tgccgatcag gtcggtgcgc ctggccagcg acgtgccgcc ggccgagcgc
      901 agcgagctgg aagtgctgcg cagtgacacg ccgaccttcg ccgccttcgt cgacgcgcgg
      961 cgcaaccggc gcgaggcctg gttccaggac ccggtgggcc atatcgagct gtgcaacgtt
     1021 cccgtggtgg tgcgcgcccc cgccgccggc cgctgatgct acgaaaaacg tagcaccgca
     1081 cccggcaaac tgatgttatt aaatatatta taaataaaaa ataatatgat atttaataac
     1141 attgattggg gcggtgcggt ggagagattt tcggcaattt gcagatggca gcggggcgcc
     1201 accaggcttg cgctgctgcg taccggcatc atctaatcgc ttgccgcagc gggggctgcg
     1261 tactttcggg ggaacagact tgaaccaggc tcaagcatcg atcgatgcca cgcaggaact
     1321 gcccgctgca tccagcgaac cactctactt cgcggtgtcg accaggaagc tggcggtgat
     1381 gtccgtggtc accctgggcc tctacgaggt atggtggttc tcctggaact ggcggctgat
     1441 caagcagcgc gaacggtccg acatcatgcc ggtgggccgc gccatcttcg cgctgttctt
     1501 cgtctatgac ctgttcaagc gcatctgcgc cagcgccgaa gaacatggag cggccaggac
     1561 atcggccggc gccctggcta gcggctggat cgtgctgtcg ctgacctggc ggctgccgga
     1621 cccgtactgg atcatttcca tgttcgcttt cgtctgcctg ctgccgatgc agaagctggt
     1681 gaaccagatc aaccagcgcg ttgcgccggc gcatgagccg aatgaccgct tcagcggctg
     1741 gaacattttc ggcatcgtcg ttggcggcat catgatgctg ctcgcgatcg tcggcgcctt
     1801 cctgcccgat cccggctgaa cgcggcgagg tgcctgcctg atcgcgcgct agccgcgggc
     1861 cagcgcgcgg ttcacctgct ccaccacgct tccgtcgcgc accagcgcgc tcaccgtttc
     1921 catgtgcggg tacatcaccg cgtcgcgctc gatcgacggc acgcggctgc ggatcagcgc
     1981 gtaagccgcg cgcgtgccgc gtccgagcgt ggcgcgccgg cgcagcgcgc gatggcggaa
     2041 gtcgatgccc tgcgccgcgc tgagcaactc gatggccagg atgcgctcca cgttgtcggc
     2101 aatggcgcgc agcttgagcc ccgcggtcac gcccatgctg acgtggtcct cgacattggc
     2161 cgaggtggga atggtgtcga cgctcgccgg gtgcgccagc accttgttct cggtggccag
     2221 cgccgcggcg gtgtactgca cgatcatgaa gccggagttg agcccgccct gctcggtgag
     2281 aaacgccggc agcacgtgct tgttcgaggc ctcgtcggtc aggcgcatgg tgcgccgctc
     2341 cgagatgttg cccagctccg ccagcgcgat gccgagataa tccatggcga tggccagggg
     2401 ctcgccgtgg aaattgccgc cggacagcac ctcgactttc ttcccttcga cgaacagcag
     2461 cgggttgtcg gtgaccgcgt tgagctccat ctcgaagatc cagcgcgcat aggcgatggc
     2521 gtcgcgcacc gcgccgtgca cctgcggaat gcagcgcagc gtgtaggcgt cctgcacgtc
     2581 gttggactgg tgcgcgcgcg tcagccggct gccggcgagc aggcggcgca ggtaggcggc
     2641 gcattccacc tggcgcggca tggcgcgcag cgcgtggatg cgtggatcga aggcgaggtc
     2701 ggtgccgttg agcgcttcca gcgacaggca gccggcgata tcggcggtct gcgacatgcg
     2761 ctcggcgcgc agcgtttcca gcacgcccag cgcgcacatg accgaggtgc cgttggtcag
     2821 cgccaggcct tccttggcgg cgagcgtcag cggctggagt tttgccagcg ccagcgcctc
     2881 ggcgctgctg aaggtctgtc cgaaatattc cgcctcgcct tcgccgatca gcgtgagcga
     2941 catgtgcgcg agcggggcca ggtcgccgct ggcgccgagc gaaccctttt ccgggatcag
     3001 cggatgcacg ccgcggttga gcaattcgag caacagctcg agggttgatg ggcgtacacc
     3061 ggagtagccg cgcgaaagtg tattggcgcg gatcagcatg atggcgcgcg tggtcgggat
     3121 gtcgtagtgc cggcccacgc ccaccgcatg gctgacgatg atgttccgct gcagcgtttc
     3181 cacgtcggcc ggtgaaatca ccttgtcctt gaacgcgccg aagccggtgg tgatgccata
     3241 ggcgatgcgg ccttccttga gcagccgctg tacgccggcg gccgcgcgcg ccacgcgctt
     3301 gcgcgccgtg cgtgccagcg tcacgcgcgg gtgcccgggc ttgccgtacg ccaccgcgcg
     3361 cacctgggcg aaactcaggc tggcgccatc cagttcgata tctttcatgg gcggattctc
     3421 acacgacgat gcggccgcgc ttgaccacgc gcgccacggc attgccgccg aactcgtagg
     3481 cgagcacgcg ccagtcagtg gcgtcgagga ggatcaggtc ggcctgcctg ccggcctgca
     3541 gcgagccggt gcgttcgccc agtccgacgg catacgccgc attgatggtg caggcgttga
     3601 gcgcttccgc cggcgccagt ttctggtagc ggcaggcgat ggccatggcc atcggcagcg
     3661 acgggcaggg cgccgagccg ggattgatgt cggtggtcag cgccagcgcg cagccggcgt
     3721 cgagcatggc gcgggcattg gcgtagcgcg tggcgcccag gtggaagttc accgtgggaa
     3781 tgatcacgcc gatgctgttg gacgcggcca gccgcgcgat gccgtcgccg tccgtttcgt
     3841 cgaggtgatc gaccgacacc gcgcccagac cgagcgccat gtccagcgcg ccgagctggc
     3901 tgaattcatc gacatgcgcc ttcagcggca ggcccagcgc ctgcgccgct tccagcacgc
     3961 ggcgcgactg cgcgagggtg aatgcatgct gctcgcagaa cacgtcgacg aacatcggca
     4021 cgcgctgagc ggcaaagcgt gactggcgat accactccgc cgccgccggc agcatctccg
     4081 ccagcagcag atcgatgtag tcgtcgccgc gtccgcgaaa ctccggcggc accgcgtgcg
     4141 cgccgaggaa cgtcggcacc aggtcaatgg cgtggttctt gtccagcgcc tcggtcgcca
     4201 gcagcagctt gagctcgctg tcggcatcga ggccgtagcc ggtcttggcc tcggcagtgg
     4261 tggtgcccag cgccagcatc cggtcgagcc gccggctgga ttcgatcacg atctgcgcca
     4321 gagtggcagc gcgcgtggcg ctgacggtgg agacgatgcc gccgccggac gccgcgatgt
     4381 cctggtaact ctcgccgcgg atgcgccgct cgaattcgcc gatgcggtcg cccgcgaaca
     4441 ccacgtgcgt gtgcggatcg accagtcccg ggcaaatcac cttgccccgg gcatcgatct
     4501 cgttgtctgc cttgaagtgt ctgcacagtt cagcggactt gccgatctgg atgatcttct
     4561 ccccgccgat ggcgatggcg gcgtcttcga tcaggccgac gtcggtcatc gcggcgccgc
     4621 gcttggccgt gggccaggcg caggtcagca gttgcgcggc gttgtagatg agcaggtcgg
     4681 cagcttgcat ggttgccggc tagggcatcg ggatcttcac gccatgcgtg cgcgcaaact
     4741 cgcgcgcctg gtcgtagccg gcatccgcgt gccgcgccac gcccatgccc ggatcgctgg
     4801 tcagcacgcg ctgcaggcgg cgcgcggcgg cgggcgtgcc gtcggccacg atcacctggc
     4861 cggcatggat gctgaagccg atgccgacgc cgccaccatg gtggatcgac acccaggtgg
     4921 cgccgcccac ggcattgatc atggcgttga gcagcggcca gtcggcgatg gcgtcgctgc
     4981 cgtccttcat cgcctcggtt tcgcggttgg ggctggccac cgagccgctg tcgagatgat
     5041 cgcggccgat cacgaccggc gccttcagtt cgccgctcgc caccatctcg ttgaacttga
     5101 gcccggcctt tgcgcgctcg ccgtagccca gccagcagat gcgggcggga aggccctgga
     5161 acttcacctg ctcgcgcgcc atcttcagcc agcgatgcag gtgcgcgttg ccgggaaaca
     5221 gctccatcac tgcgcggtcg gtccggtaga tgtcttccgc gtcgccggac agcgcgaccc
     5281 agcggaacgg ccccttgcct tcgcagaaca gcgggcggat gtaggccggc acgaagccgg
     5341 ggtagtcgaa tgcgtgcttc acgccgtgat cgagcgcgcg ctggcgcagg ttgttgccgt
     5401 agtcgaatac cacggctccc tgtttctgga acgaaagcat cgcgcgcacg tgtgcggcca
     5461 tgctgtccat cgcgcgtcgt tcgtattcag cctgatcgct cttgcgcaga ttctcggcat
     5521 cgcccaacga caggcctgcg gggatgtagc cgtagagcac gtcatgcgcc gaggtctggt
     5581 cggtgaccac gtcgggcttc acgccgcgtt tcaggatttc cggcaggacg tccgcggcgt
     5641 ttccgatcag gccgatggac ttggctatgc ctcgttcgca ggcgtcgcgt gcccaggcca
     5701 acgcttcgtc caggctggcg gtgccaacgt cgagctgctt gagttccagc cgccgctgca
     5761 cgcgccacgg atcgacttcc accagcaggc cgacgccttc attcatggtg atggccagcg
     5821 gctgcgcgcc gcccatttca cccaggcccg cggtcagcac cagcttgccc ttgagcgtgc
     5881 cccagccgtg ctggcgcgcg agtgcgccga aggtttcgta ggtgccctgc aagattccct
     5941 gcgtgccgat gtagatccac gagcccgcgg tcatctggcc gaacatgatc aggccttcgt
     6001 gttcccaccg gtcgaagttc tgctgagtgg cccagtgcgg cacgatgttg gagttggcca
     6061 gcagcacgcg cggcgcgtcc tcgtgcgtgc ggaacacggc gaccggcttg ccggactgca
     6121 ccagcagcgt ttcatccggt tcgagttttt tcagcgacgc aagtatggcg tcgtagcagt
     6181 cccagttgcg cgcggcgcgg ccgcggccac cgtagacgat gaggttgtcg gaatcgaagg
     6241 caacctgcgg gtcgaggttg ttctggatca tccggtaggc ggcttcgatc agccagttcc
     6301 tgcagctgag cgtcgacccg gtgggcgcgt ggatttcgcg cttcatgtcc gcctccttac
     6361 aacgtggctg ccagcgccct caggtagtga tgcatggcga ccgcggtcag ccggctggtg
     6421 cgggcttcca ggtcgtgcat cgggttgacc tcggagaatt cgaccacgcg cgtgcgcgga
     6481 tcgcgccccg cgatctccgc gatctggcag aactcgcgcg ag
//
LOCUS       DASZZC010000491         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1006832,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000491 DASZZC010000000
VERSION     DASZZC010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006832"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VHE37_10410"
     CDS             <1..478
                     /locus_tag="VHE37_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HVT35990.1"
                     /translation="GNEDDLQAPYLYAFLGAPWKNAVVARSASSIYTPTPLGLPGNDD
                     LGALSGWLVWNMLGVYPFNPGTPLFVIGSPQFEHAVMRRPSGDLRIDAPGAAEFVTGT
                     TLNNNPLARTWFVLPRAATTLALDTAALPDMNWGADASAAPPSISSGTLADFGCVQ"
     gene            complement(581..829)
                     /locus_tag="VHE37_10415"
     CDS             complement(581..829)
                     /locus_tag="VHE37_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35991.1"
                     /translation="MKSVAGMLALVLLAGCSGPTAQLHLSAGRIGCSTKDIVIGDVAS
                     TRHSETWSASCRGQVWYCSANDDFREVVCNPASAPAQK"
     gene            complement(837..>1705)
                     /locus_tag="VHE37_10420"
     CDS             complement(837..>1705)
                     /locus_tag="VHE37_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT35992.1"
                     /translation="RSADDVLGPVDAQHPMDAAPASTPAPASTAEPASVPVAAPAASP
                     PAAGAPEDSGAHHGIAAALRVSVLQGIGIEGIYGLSPYFNVRGQFNTFSYNRNFNKDG
                     VDYDGKLKLQSIGALIDYHPFAGHFRLSLGLYNDGNKVKAHATCQPGNQCNVGDFIVT
                     ANPGDNPDLSAGLDMSRGVAPYFGFGWGNAMTGWPIHFGFDIGVMYQGKPNFSLAASG
                     KAKVTNSDGVTCADPTTVGAGCEISDLSSNATFQSELAKQQQKTQSDIDGEAAAKWWP
                     SIAFTIGYRFGG"
BASE COUNT          279 a          540 c          579 g          307 t
ORIGIN      
        1 cggcaacgag gacgacctgc aggcgccgta tctctacgcg tttctcggcg cgccgtggaa
       61 gaacgcggtg gtggcgcgct cggcctcgtc gatctacacg cccacgccgc tgggcctgcc
      121 gggcaacgat gacctgggcg cgctgtcggg ctggctggtg tggaacatgc tcggcgtcta
      181 tccgttcaac ccgggcacgc cgctgttcgt gatcggcagc ccgcagttcg agcacgcggt
      241 gatgcgccgg ccgtccggcg acctgcgcat cgacgcgccg ggcgcggcgg agttcgtcac
      301 cggcacgacg ctgaacaaca atccgcttgc gaggacatgg ttcgtgctgc cgcgcgcggc
      361 gaccacgctg gcgctggaca cggcggcgct gccggacatg aactggggtg cggatgccag
      421 cgccgcgccg ccttcgatca gcagcggcac gctcgccgat ttcggctgcg tgcagtgaat
      481 ttatggagcc accgcactgc catttcttgt gttattaaat attgtttaaa tttatataca
      541 gacaataatt aataacagca acaagggcgg tgcggtgagg ctatttctgc gccggtgccg
      601 acgccggatt gcacaccact tcgcggaagt cgtcgttggc cgagcagtac cagacctggc
      661 cgcggcagct ggccgaccag gtttccgagt ggcgcgtgga cgccacgtcg ccgatgacga
      721 tgtccttggt cgagcagccg atgcgcccgg ccgacaggtg cagctgcgcg gtggggccgc
      781 tgcagcccgc gagcaaaacc agcgccagca tccctgctac ggatttcata gcgcgctcag
      841 ccaccgaagc ggtaaccgat ggtgaaggcg atcgacggcc accatttcgc cgctgcttcg
      901 ccgtcgatgt ccgactgggt tttctgctgc tgcttggcca gttccgactg gaacgtggca
      961 ttgctcgaca ggtcggaaat ctcgcaccct gctccaacgg ttgtcggatc ggcacaggtg
     1021 acgccatcgc tgttggtcac cttggccttg cccgacgcgg ccagcgagaa gttcggcttg
     1081 ccctgataca tcacgccgat gtcgaagccg aagtggatcg gccaacccgt catcgcgttg
     1141 ccccagccga agccgaagta cggggccacg ccgcggctca tgtcgagccc ggccgaaaga
     1201 tcagggttgt cgccgggatt ggcggtgacg atgaagtcgc cgacgttgca ctggttgccg
     1261 ggctggcacg tggcatgcgc cttcaccttg ttgccgtcgt tgtacaggcc caggctcagg
     1321 cggaagtggc cggcgaacgg gtggtaatcg atcagcgcgc cgatgctctg cagcttgagc
     1381 ttgccgtcgt aatcgacgcc gtccttgttg aagttgcggt tgtagctgaa ggtgttgaac
     1441 tgcccgcgca cattgaagta gggcgacagg ccgtagatgc cctcgatgcc gatgccctgc
     1501 agcaccgaca cgcgcagcgc cgcggcaatg ccgtgatgcg caccggaatc ttcgggcgcg
     1561 ccggcggccg gcggcgatgc cgcgggcgca gccacgggaa cggatgcggg ttctgccgtg
     1621 gacgccggcg ctggcgtcga tgcaggcgcg gcgtccatcg gatgctgcgc gtcgaccggc
     1681 ccaagcacgt catcggcgct gcggg
//
LOCUS       DASZZC010000492         4219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_555070,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000492 DASZZC010000000
VERSION     DASZZC010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555070"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VHE37_10425"
     CDS             <1..1261
                     /locus_tag="VHE37_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35993.1"
                     /translation="YRKTAALERMNRELEQRVAERTAELERDLATRKQLEEALVDAGR
                     RKDEFLAVLAHELRNPLAPIRTAVELMHMRPLSDPAVVHSRDVIGRQVEHLTRLVDDL
                     LDVSRITRGKIRLEMEPLEIEAAVNRAIETNRPIFESRGQRLTVRVPDSPVIVRADMT
                     RLSEAIGNLLNNAAKYTDEGGEIVLSVDLSDDARLVAVRVKDSGMGIPPEMLPKVFEL
                     FTQVDHTLHRAQGGLGIGLALVRRLMEMHGGTVEGHSDGVGKGSEFVLRLPVHDQAVA
                     AAAARPEAGGLGRPLKRILVVDHNRDAAETLAMVLRLNGGEVETAHDGREAVEAAERF
                     RPHVVLLDLGMPGINGLEVARTIRAQPWGSDMTLVAQTGWGQEEDRRRTTEAGFDDHL
                     TKPMDHSKLLQLLARPSPRAAQVSASR"
     gene            1330..1806
                     /locus_tag="VHE37_10430"
     CDS             1330..1806
                     /locus_tag="VHE37_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT35994.1"
                     /translation="MDHQDHDRVFVINFGIVLGALMGLALVFVVVARIASAGDNKQSG
                     EALAMLDERIKPIGHAVTDPSVLLQQQAAKPAHTPMTGEQVVAKVCSACHGTGMLGAP
                     KIGDKAAWGPRKSAAGGIDGLVASAGKGKNQMPPRGGDAELSDAELKAAIEQMAGK"
     gene            1820..2530
                     /locus_tag="VHE37_10435"
     CDS             1820..2530
                     /locus_tag="VHE37_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HVT35995.1"
                     /translation="MGGCARKAPPVLAALLLCCPLAHAGLKGSAGAVSEYLFRGIESS
                     GGAAVQGDAYYAWDEGYYAGAWLSNTVHAATQIDSYAGYDRRFGDFRAGGGVVYRYFS
                     EEVSTASTANHQDRISFGELYLTGGYGPANGAVYYGHDYFGSSKDSLYVTGNVNLPVR
                     ETVSVILEGGYSSGPGITTVWSGRFFDYRASLEKRFDDGFSAGFHVAGTTRGATAGEF
                     SIDNRPKFLVSFKKDFDF"
     gene            2715..3803
                     /locus_tag="VHE37_10440"
     CDS             2715..3803
                     /locus_tag="VHE37_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVT35996.1"
                     /translation="MFKLKTVVAAAGLMALPALSQAAAPTLSDVLDASSISVTGHATG
                     SYAYQKVNPPAPAASSAVNSFGFDQAELMVSKLPASGFGAAVDVFAGQDVTGGSINYG
                     GLPGGAPTVAAFNKFGAGGEINLHQAYVQYATGALTVIGGKYATLAGSEVASDVLNSN
                     ATRSILFDNQPFTLTGVRAGYKFSDMITGYLGFANSAGGATADFNDQKTTEIGVALAP
                     LTGLTVNVTDYIGNEGAAGSGNKTNLLDLVVGYTAGDLNVGLNADRNTSHGPAAVDLT
                     FTGVALYANYQVMPSFRAGRRGEITTTKDDVAATKTKQDEVTLTGDYSGGKNFDLVTD
                     LRYDALKFEPSGSTAHAVTFLTKAIFKF"
     gene            complement(3852..>4219)
                     /locus_tag="VHE37_10445"
     CDS             complement(3852..>4219)
                     /locus_tag="VHE37_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="among the AAA+ ATPases, the YifB protease family
                     belongs to the Helix 2 insert clade; unknown function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HVT35997.1"
                     /translation="IDLQLFVSRVERDALSSRQPAAAETSAEVRQRVVDARRRQLARQ
                     GKPNHQLQPKEIDLHCAPEAEAHQLLLTTMSRLQLSARAYHRVLKVARTIADLAACGP
                     ILAAHVAEAVRYRELDRGA"
BASE COUNT          777 a         1339 c         1355 g          748 t
ORIGIN      
        1 ctatcgcaag acggccgcac tcgagcgcat gaaccgtgaa ctggaacagc gcgtggccga
       61 acgcactgcc gagcttgagc gcgacctggc gacgcgcaag cagctggagg aagcactggt
      121 cgatgccggc cggcgcaagg atgagttcct tgccgtgctg gcccatgagc tgcgcaatcc
      181 gctcgcgccc atccgcaccg cggtcgaact gatgcacatg cggccgctca gcgatccggc
      241 cgtcgtccac agccgcgatg tcatcggccg ccaggtcgaa cacctgaccc ggctggtgga
      301 cgatttgctc gacgtgtcgc gcatcacccg cggcaagatc cgcctggaga tggagccgtt
      361 ggaaatcgag gcggcggtga atcgggccat cgagaccaat cgcccgatct tcgaaagccg
      421 cggccagcgc ctgacggtac gcgtgcccga ctcgccggtg atcgtccgcg cggacatgac
      481 acggttgtcc gaagcgatcg gcaacctgct caacaacgcg gccaagtaca ccgatgaagg
      541 cggcgaaatc gtcctgtctg tcgacttgtc cgacgatgcg cggctggtgg ccgtgcgcgt
      601 gaaggacagc ggcatgggca tcccgccgga aatgctgccg aaggtattcg agctgttcac
      661 ccaggtcgac catacgctgc atcgcgcgca gggcggcctg ggcatcggac tggcactggt
      721 gcgcaggctg atggaaatgc acggcggcac ggtggagggc cacagcgacg gcgtgggcaa
      781 gggaagcgag ttcgtcctgc gcctgccggt gcatgaccag gcggtcgccg ctgcagccgc
      841 gcggccggaa gccggagggc tcggccgtcc cttgaaacgg atcctggtgg tggatcacaa
      901 ccgcgacgcc gcggaaacgc tggccatggt gctgcgcctc aatggcgggg aggttgaaac
      961 cgcacacgat ggccgcgagg cggtggaagc ggccgagcgc ttccggcccc acgtggtctt
     1021 gcttgacctg ggcatgccag gcatcaacgg cctggaagtg gcccgcacca ttcgtgccca
     1081 gccgtgggga tcggacatga cgctggttgc gcagaccggc tgggggcagg aggaagaccg
     1141 caggcgcacc accgaagccg gcttcgacga ccacctgacc aagccgatgg atcattcaaa
     1201 actcctgcag ctgctggcca ggccctcgcc ccgcgcggcg caggtatccg cgtcccgctg
     1261 attaccggcc cgcgcgaacg cgcgcgtgca tcggatataa tttttgaatc agcgattggg
     1321 agcgttcctt tggatcatca ggaccacgac agggtatttg tcatcaattt cggcatcgtg
     1381 ctgggcgcac tgatgggtct ggccctcgtg ttcgtcgtgg tggcgcgtat tgcctctgcg
     1441 ggcgacaaca agcagagcgg cgaagccctg gccatgctcg atgagcgcat caagcccatc
     1501 ggccacgccg tgaccgatcc gtcggtcctg ctccagcaac aggccgccaa gccggcccat
     1561 acgccaatga ccggcgagca ggtggtggcc aaggtgtgca gcgcctgcca cggcaccggc
     1621 atgctcggcg cgccgaagat cggcgacaag gccgcctggg gtccgcgcaa gtcggcggca
     1681 ggcggcatcg atggactggt ggcatcggcg ggcaaaggca agaaccagat gcccccccgc
     1741 ggtggtgatg cggaactctc cgacgccgag ttgaaagcgg cgatcgagca gatggcgggc
     1801 aagtaacccg ctgcaatcca tgggcggctg cgcgagaaag gccccgcccg tccttgccgc
     1861 cctgttgctc tgctgcccgc tcgcccatgc gggactgaaa ggcagcgcag gcgctgtcag
     1921 cgaatacctg ttccgcggca tcgagagcag cggtggcgct gccgtgcaag gggatgccta
     1981 ttacgcctgg gacgagggct attacgccgg cgcctggctc agcaacaccg tgcatgccgc
     2041 tacacagata gacagttacg ccggctatga ccggcgcttc ggcgatttcc gtgccggtgg
     2101 cggcgtggtc tatcgctatt tcagcgaaga agtttcgacc gcgagcacgg cgaaccacca
     2161 ggaccgcatc agcttcgggg agctctacct gaccggcgga tatggccctg ccaacggcgc
     2221 cgtgtattac ggccatgatt acttcggctc ctccaaggat tcgctctacg tcaccggcaa
     2281 cgtcaacctg ccggtccgtg aaactgtctc ggtgatcctg gagggcggat acagttcggg
     2341 tccgggaatc accacggtct ggagcggtcg gttcttcgat taccgcgctt cactggagaa
     2401 gcgtttcgac gatgggttct ccgcgggctt ccacgtggcg ggaaccacgc ggggagccac
     2461 tgcgggcgaa ttctcgatcg acaaccggcc gaaattcctg gtctcgttca agaaggattt
     2521 cgatttctaa tgtcttcatc gagcaagggt ggcatggccc ttgctcattt cttggcgcgt
     2581 cgaaacggcc tttgacatca agggttccgc gcctactgcg ttgcagcatg tatatgttac
     2641 agtgctgatg cagtgatgat tccggccgcc tcctgggcgg cttttgctta cctaaaacct
     2701 tcatgggaga ggcaatgttt aaactcaaga cagttgttgc tgccgcgggt ctgatggcgc
     2761 tgcctgcgct gagccaggcc gctgcgccca cgttgtccga cgtgctcgat gcttccagca
     2821 tttccgtcac cggccatgcg accggctcgt atgcttacca gaaagtaaat ccccccgctc
     2881 cggctgcatc gtcggcggtc aactccttcg gattcgatca ggctgaactg atggtctcca
     2941 agctgccggc cagtggcttc ggtgcggccg tggacgtctt cgccggccag gatgtgacgg
     3001 gtggcagcat caactacggc ggtctgcccg gcggcgctcc gacggttgca gccttcaaca
     3061 agttcggtgc gggcggtgaa atcaatctgc atcaggcata cgtccagtac gccaccgggg
     3121 cgctgacggt gattggcggc aagtacgcca ctctcgccgg ctccgaagtg gcatcggacg
     3181 tgctgaattc caacgcgacc cgatcgatcc tgttcgacaa tcagcctttc acgctaaccg
     3241 gcgtgcgcgc tggctacaag ttcagcgaca tgatcaccgg ctaccttgga ttcgccaaca
     3301 gcgccggcgg cgcgaccgcg gatttcaatg accagaagac caccgaaatc ggcgtggcgc
     3361 tggctccgct gacgggcctc acggtcaacg tcaccgacta catcggcaac gaaggcgccg
     3421 cgggttccgg caacaagacc aatctgctcg atctcgtggt cggctacacc gctggcgacc
     3481 tgaacgtcgg cctaaatgcg gaccgcaaca ccagccacgg tccggccgcg gttgatttga
     3541 ccttcactgg cgttgcgctc tatgccaact accaggtgat gccgtcgttc cgcgccggcc
     3601 ggcgcggcga aatcaccacg acgaaggatg acgttgccgc taccaagacc aagcaggacg
     3661 aagtcacgct gaccggagac tattccggcg gtaagaactt cgacctggtc accgacctgc
     3721 gttacgacgc tctgaagttt gaaccttccg gttccacggc tcacgccgtg accttcctga
     3781 cgaaggcgat cttcaagttc taaacttgtg gttgtgaatg aagggccgcg caagcggccc
     3841 tttttttgtc ttcaagcacc acgatcgagt tcgcggtagc gtacggcctc ggcgacatgc
     3901 gccgccagaa tcggcccaca ggctgcgagg tcggcaatgg tccgcgccac cttcagcacc
     3961 cggtgatagg cgcgcgccga cagctgaaga cgcgacatgg tggtgagcaa cagctgatgc
     4021 gcttcggctt caggcgcgca atgcaggtcg atctccttgg gctgcagttg gtgattgggc
     4081 ttgccctggc gcgccagctg gcgccgacgc gcatcgacca cgcgctggcg cacctctgcg
     4141 ctcgtctcgg ccgcagccgg ctggcgcgat gacagggcat cacgctcaac gcgcgagacg
     4201 aacagctgca aatcgatcc
//
LOCUS       DASZZC010000493         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_295683,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000493 DASZZC010000000
VERSION     DASZZC010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295683"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VHE37_10450"
     CDS             <1..917
                     /locus_tag="VHE37_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT35998.1"
                     /translation="TAALGEVERVFASTATAGATVSSFETPMRRPDGSERIITFSIAP
                     LMRNGRVAGVAGTAEDITERRRASAELAARVDELARSNAELEQFAYAASHDLQEPLRT
                     VNSFAQLLARRHVSNDPEAAEFIGYITEAVARMRALIDGLLAYSRVRPQDTEPETVDL
                     DRLLAQVLADLDDAVVRSGAEVTHDALPTLKGDAAQLAQLLQNLVSNALKFRAPDRAP
                     RVHVGAQRQEHGWLLTVRDNGIGIEPRYAGRVFGLFQRLHTRDLYPGSGIGLAICKKI
                     VESHGGTIRVEAGNPGSVFVFTWPDVRA"
     gene            914..1837
                     /locus_tag="VHE37_10455"
     CDS             914..1837
                     /locus_tag="VHE37_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT35999.1"
                     /translation="MKRLVAALLLYCAGVAANPPTAADFVPRRIPLSHPLSWDEVLRL
                     PPPPPVPRMAYGPAPQQFGELRVPVGAGPFPVALLVHGGCWQAGYAYRYFSHLAAWLA
                     ARGIASWTVEYRRLGDAGGGWPNTFLDVAAAADALRALAQQQPLDLTHVVAAGHSAGG
                     QLALWLAARAQLPASGELHAAQPLPIAGVVALAPITDLAAYRIGPPDSCNASVEKLLG
                     GTPQQQPQRYAQASPVELLPLRVPQWIVQGSDDRIVPAAGVRAYAQRARAAGDRVELA
                     ELPRLGHFDVAVPGTGAEAALTRAFDQAAGR"
     gene            1901..2308
                     /locus_tag="VHE37_10460"
     CDS             1901..2308
                     /locus_tag="VHE37_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36000.1"
                     /translation="MNRMIRIGIACAALALTAAATPAAAGYYVSGSYYGGYGPGWHGG
                     YWYHGWHGGRGGWWWIVGPSWYWYAEPVYPYPEHVVVEEREPPMTGPAPAPSWYYCRS
                     SNAYYPYVSSCAEGWEQVPATPPPPSPSNEKSK"
     gene            2308..>2599
                     /locus_tag="VHE37_10465"
     CDS             2308..>2599
                     /locus_tag="VHE37_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36001.1"
                     /translation="MIRHAAAVVAASAVAVLAGCAQMPTGPSVAVMPAPNKPFEVFMQ
                     DEQMCRQYAASQLGNSADQANGSAAGKAVGGAALGAAAGALMANNSRGAGVGA"
BASE COUNT          366 a          925 c          921 g          387 t
ORIGIN      
        1 acaccgccgc gctaggcgag gtcgagcgcg tgtttgccag caccgcgacg gccggcgcca
       61 ccgtcagcag cttcgagacg ccgatgcgcc gcccggatgg cagcgagcgc atcatcacct
      121 tcagcatcgc gccgctgatg cgcaacggcc gcgtggccgg cgtggccggc accgccgagg
      181 acatcactga gcgcaggcgc gccagcgccg aactggcggc gcgcgtggac gagctggcgc
      241 gctccaacgc cgagctggag cagttcgcct acgccgcctc gcacgacctg caggagccgc
      301 tgcgcacggt gaacagcttt gcccagctgc tggcgcgccg gcatgtcagc aacgacccgg
      361 aagccgccga gttcatcggc tacatcaccg aggcggtggc gcgcatgcgt gcgctgatcg
      421 acggcctgct ggcctattcg cgcgtgcgcc cgcaggacac cgagccggag acggtggacc
      481 tcgaccgcct gctggcacag gtgctggcgg acctggacga cgcggtggtg cgcagcggcg
      541 ccgaggtgac gcacgatgca ttgcccacgc tcaagggtga tgcggcgcag ttggcacagc
      601 tgctgcagaa cctggtttcc aacgcgctca agttccgcgc gccggaccgc gcgccgcgcg
      661 tacatgtcgg cgcgcagcgg caggagcacg gctggctgct gacggtgcgc gacaacggca
      721 ttggcatcga gccgcgctac gccgggcgcg tgttcggcct gttccagcgc ctgcacacgc
      781 gcgaccttta tcccggcagc ggcatcggcc tggccatctg caagaagatc gtcgagagcc
      841 acggcggcac catccgcgtt gaagccggca acccgggctc ggtgttcgtg ttcacctggc
      901 cggatgtgcg agcgtgaaac ggctcgtcgc cgcgctcctg ctgtactgcg ccggcgtcgc
      961 cgccaacccg ccgaccgccg ccgatttcgt cccgaggaga attcccttga gccatccgct
     1021 gagctgggac gaggtgctgc gcctcccgcc gccgccgccg gtgccgcgca tggcctatgg
     1081 accggcgccg cagcagttcg gcgaactgcg cgtgcccgtc ggcgccggcc cgttcccggt
     1141 ggcgctgctg gtgcacggcg gctgctggca ggccgggtac gcctaccgct atttctcgca
     1201 cctcgcggcc tggctggcgg cacgcggcat cgccagctgg accgtcgaat atcgccggct
     1261 gggcgatgcc ggcggcggct ggcccaatac ttttctcgac gttgcagcgg cggccgatgc
     1321 gttgcgcgcg ctggcgcagc agcaaccgct tgacctgacc catgtggtcg cggccggtca
     1381 ttccgccggc ggccagctgg cgctgtggct ggcggcgcgc gcacagctgc cggcgtcggg
     1441 cgagctccac gcggcgcagc cgctgccgat cgccggcgtg gtggcgctgg cgccgatcac
     1501 cgacctggcc gcgtatcgca ttggcccgcc ggacagctgc aatgcctcgg tggaaaagtt
     1561 gctcggcggc acaccacagc agcagccgca gcgctacgcg caggcttcgc cggtcgaact
     1621 gctgccgctg cgcgtgccgc aatggatcgt gcaggggagc gatgatcgca tcgtgcccgc
     1681 ggccggcgtg cgcgcgtatg cgcagcgcgc gcgcgcggcc ggtgatcgcg tcgagctcgc
     1741 ggaactgccg cgcctgggcc acttcgacgt ggccgtgccg ggcaccggcg ccgaagcggc
     1801 gctgacccgc gcctttgacc aggccgccgg gcggtgaacg ttcaggattg attcaggtca
     1861 atgtgatgtt cttgccccga tccacaatct cgacatgccc atgaacagaa tgatccgcat
     1921 cggcatcgcc tgcgcagcgc tcgcgctgac cgcggctgcc acgcccgcgg cagcgggcta
     1981 ttacgtctcc ggcagctact atggcggcta cggccccggc tggcacggcg gctactggta
     2041 ccacggctgg catggcggcc gcggcggctg gtggtggatc gtcggtccga gctggtactg
     2101 gtacgcagag ccggtgtatc cctatcccga gcacgtggtg gtggaagaac gcgagccgcc
     2161 gatgaccggc cccgcgccgg cgccgtcgtg gtactactgc cgctcgtcga atgcctatta
     2221 cccttatgtg tccagctgcg cggaaggctg ggagcaggtt cccgccacgc cgccgccacc
     2281 ttcaccctcc aacgagaaga gcaaatgatg atccgtcacg ctgccgccgt ggttgccgcc
     2341 tccgctgtcg ccgtgctcgc cggttgcgcg cagatgccca ccgggcctag cgtcgccgtg
     2401 atgccggcgc cgaacaaacc tttcgaagtg ttcatgcagg acgagcagat gtgccgccag
     2461 tacgccgcct cgcagctggg caactcggcc gaccaggcca acggcagcgc ggccggcaag
     2521 gcggtgggcg gtgctgcgct gggcgcggcg gccggcgcgc tgatggccaa caacagccgt
     2581 ggcgccggcg tgggcgcgg
//
LOCUS       DASZZC010000494         7730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_168996,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000494 DASZZC010000000
VERSION     DASZZC010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168996"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..443)
                     /locus_tag="VHE37_10470"
     CDS             complement(126..443)
                     /locus_tag="VHE37_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36002.1"
                     /translation="MPIHIGDLYVILNNARPGESCLATAVILPEVSGKAWYLLSDLQV
                     YYPLESVHKFELETGFKLVFIRRARDKSLAQAAQERVPPEFRKYLEYLGERSCAAPAE
                     LML"
     gene            complement(778..1623)
                     /locus_tag="VHE37_10475"
     CDS             complement(778..1623)
                     /locus_tag="VHE37_10475"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HVT36003.1"
                     /translation="MKYPIALLAAALSAPLLASSPEATVELLAENTSLHPGQNWIGLS
                     IQPASGWHVYWQNPGDVGQPTQIELVTPPGVSAGDMRWPVPRVRTEAGMTSFVYEQPV
                     LHLIPVTVDARAPRIVDFTASVSYVVCHDTCIPQHADLKLSLPVAKTAKVDRANTDDF
                     AKARETTPIDKADWIASYDMTGDLLTVEICGDGAGKLGSPAVFPLADELVNYAVAPRY
                     ESNDENHLRLLLPLSKTHAAVHGPLGFVLAGPSAVAITASAQPGLTRTIAMTRQVRGA
                     CLAAG"
     gene            complement(1738..4176)
                     /gene="csf4"
                     /locus_tag="VHE37_10480"
     CDS             complement(1738..4176)
                     /gene="csf4"
                     /locus_tag="VHE37_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV CRISPR-associated DEAD/DEAH-box helicase
                     Csf4"
                     /protein_id="HVT36004.1"
                     /translation="MPTNSIVTRTISLPLADLELLKAKGAVSRVALSLAIEEGTKDRP
                     ILPVPQYEVLHHAPVRVPGDKLDRLREIAKAKGLKDLADGTLLSRLIITALRGDTVVA
                     IKPATPFKVDPTLLSTQERTARPEQLQFFADIAEANSVGRIILAEAATGVGKGLVIAA
                     AAHMAASAGKRVVVASPTHLISRQTRSDLAAINRSHGEVKFAVVYGRREFVSSRRLSE
                     MLDEAQARADLGHAQIAEARKWLDRQIAAGDNATWLSAELEAHCPGLAREYALHNAEV
                     GEPGEMAYQRQFVAAEKAPIVFASHAMLAHDTLARVRSALAAGAGSGERDQPKTEQPW
                     YIASNEKLISFEADRGVLPNFEVLLVDEAHLLERAFAEAASASVSVRMMVRRIDKLAN
                     SSKSPTRREAASTAKKCFERLEQLSRWSDSRLSISKHTSQPAAGRGAELVAELLAAGQ
                     LIVKSIKDNSTDEERQLLSDVDALKRAVHANEHMLVQVEFTPYRRWPTVLVGPRHVNA
                     PMDFMWRRVAAGAAVSATLYIDSSAGVPSADYMRSKLNVPRERLATHRPITPAWVTEP
                     VTLYTPEIRSGESRAHRWCPPSGTEPDEADLARYHMEIASVIESISQEQDRGGMLVLA
                     TSYAFIAAMKASGKFGDNAIYQAEGVSLEVPRERFIRLSMDGQRPLWFATGSAWTGLN
                     LAHPDASVAPEQDRILTHLVIVRAPFNTEKSSVSLARMERADGFMSEIMRCALQLKQA
                     MGRLVRRPGLSDRHLYLLDPRMVDPGMSYGAIFERLVTRYPRRKLLSQTVDPVTASRM
                     KPVPQTSKRRRGLI"
     gene            4225..4965
                     /locus_tag="VHE37_10485"
     CDS             4225..4965
                     /locus_tag="VHE37_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36005.1"
                     /translation="MIQNEVMFWMPDGRPLYPQEGRRLLGELCGDKLDPAFFHHQEED
                     RLLPKSGGVPPVRFSGNGKIFRIIGVGVVGAELVNESFATVLAALTKRFGPLKTQMAE
                     SEHALTFSAMPRRYAIRHMFLYKGIDERDKFAALDAVAKRAWLLDMVCRGLARQYEEL
                     GYDGKMPDLRPQLNAFVEHSVHPIKDGSKIHGGVADLVVTLQAKLVGHWAAGLLISKG
                     YGRLDPYLESTDPQEFLMSRFAAKMASR"
     gene            4962..5714
                     /gene="csf1"
                     /locus_tag="VHE37_10490"
     CDS             4962..5714
                     /gene="csf1"
                     /locus_tag="VHE37_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV CRISPR-associated protein Csf1"
                     /protein_id="HVT36006.1"
                     /translation="MTIQHATELVCEVAGLKPLYTSKAEAAGHCAWCARHIEQGARCS
                     TFIAPVTFNDWPILGCLTSSMLCGWCRRVQEQGKPFMQTYGRAVVSRSGFFAFASNND
                     RAAFFVNPPKPPFIVVNITAQQQHIYWKAPVNFSRDLLRIQFGDRLLTIRRQRALDAA
                     ADIVNYLTDPANHVVRPGTRKAVPPRFPISIDRDLEAPNCGEVSPDVPPALRQTLGSL
                     TTGELFALTCIVPLRGATPAPAKLLELKLKGE"
     gene            5718..6929
                     /locus_tag="VHE37_10495"
     CDS             5718..6929
                     /locus_tag="VHE37_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36007.1"
                     /translation="MKLLIEGVFTLKTPLHVAHAGMEGSVESGQIRRYRQGAVPLPLV
                     PTQQIPVFSELAGQDAATGGRGVRLPLVPIYPGSALRGRIHAAAGNDLIHDLLRRSLQ
                     LTREEYHVLRVNTFSGAGARVEPTVSGVSAAQNDLYAAVFGGGPMMLRSLLVTNDLFP
                     IISDTVSSGLIADKFVRQIDRDLQVRGENITFYQPSVRRDALLEFSDPLAPMIVKEYE
                     PALLEWQTLCSESNAARKAERESAQQKRAAKKAGMKVASEAPALDPVAPAAAAGGEAA
                     KAKKVSLKNQQAIEAVAPGVRFYVRVGLREGARSEHVGMITAALGAVIEANDLGGYSR
                     NGYGRFSAALDVTVNDATFENAITFNGNGLKIGQELKPHLEAYRTVLQSYTPEQFARA
                     YSLGNSPDASA"
     gene            6943..7572
                     /locus_tag="VHE37_10500"
     CDS             6943..7572
                     /locus_tag="VHE37_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36008.1"
                     /translation="MTFDLLTAVAAPEHPIHLDALLCGVLELRAQAQGRKVGDEVERL
                     PLAAHASGCWKASALVFDVQERFNRFATRRVDQDRLVRDIASGAVGGRGDALNFNSGP
                     FRNYLMTIPVIYARSATAWCIGDADEIRTMLLELPSIGRLGRNGYGAIGGEPVVEHDE
                     LAHRLWESRILPYEVADADEVFATVRYPYFDKTKQKKAWIPRTLSASLV"
BASE COUNT         1393 a         2274 c         2613 g         1450 t
ORIGIN      
        1 caagcgcagg cccagcgcga aaaacagcag cacgatgtgg aagccagcaa acccggcgta
       61 ggccgcaaat gagtgggacg cgcgcgcgtg atgcgccacg gagcgagtgg atttgaagcg
      121 gcggttcaca gcatcaactc cgcgggagcg gcgcacgagc gctcgcccag gtactccagg
      181 tactttcgaa actcgggtgg cacgcgttcc tgggcggcct gcgcaagtga cttgtcgcgc
      241 gcgcgccgga tgaagaccag cttgaaccct gtttcgagct cgaacttatg cacggactcc
      301 agcgggtagt agacctgcag gtccgacagc aggtaccacg ccttgccgga aacctccggc
      361 aggatgacgg cggtggccag gcaggattcc ccagggcggg cattgttgag gatcacgtac
      421 aggtcgccga tgtggatggg catcggagcg gcgaagctga ggggaggggc gggcgcgttc
      481 atgaccccat cgtcgcctgc cgcgctggac ttgccgagag ggtaaaggcg gtttgcgggc
      541 cgtttttagc cgagagcctg acggccgggg tgctgttgtg acgcatccac tgtggataac
      601 agcgaacatg aataatgcaa gcctgcggcc acggtcgggt gttgggttgg tgcgacctaa
      661 cacccaaaat attcgtggta gtagagaccg cattgtgcgg cgcgcgtggg gtggtcagcg
      721 gactgcaaaa ccccaatatc cgggtggtcg gtggccatag cctgctcggc tgatgggtta
      781 gccagcggca agacatgcgc cgcgcacttg ccgtgtcatg gcaatcgtgc gggtcaaccc
      841 tggctgcgct gatgcggtga tggctaccgc gcttgggccg gcgagcacga atcccagcgg
      901 gccgtgtacg gccgcgtgag tcttgctcag cggcaacagc aggcgcagat gattttcgtc
      961 gttggactcg tagcgcggcg cgacggcgta gttgaccagc tcgtcggcca atggaaaaac
     1021 ggcggggctg cccagcttgc cggcgccgtc gccgcaaatc tctacggtga gcagatcacc
     1081 cgtcatgtcg tacgaggcta tccaatccgc cttgtcgatg ggggtggtct cgcgagcctt
     1141 ggcaaaatcg tcagtgttgg cgcggtcgac tttcgcagtc ttggccacag ggagcgagag
     1201 cttgagatcc gcatgctgcg ggatgcacgt gtcgtggcag acaacgtagg acaccgatgc
     1261 ggtgaagtcg acgatgcgtg gcgcgcgcgc gtcaaccgtg acggggatca ggtgcagaac
     1321 gggctgctcg tacacgaagc tggtcatgcc cgcttcggtg cgcacgcgag gcaccggcca
     1381 gcgcatgtcg ccggcgctga caccaggtgg cgtgaccagc tcgatctgcg tcggctgccc
     1441 gacgtcgcct gggttttgcc aatagacatg ccagcctgac gctggttgga tggacagccc
     1501 gatccagttc tgccccggat ggagcgaggt gttttcggcc agcagttcga ccgttgcttc
     1561 cggtgaggag gcaagcagtg gggcggacag cgccgcggcc agcagggcaa tggggtactt
     1621 catgcgacca cggtacctcg cggtccaggg cgccggaacg gggctatggc ggtcgaaaat
     1681 ccgtttttat ctaaacccgt cccatcaggc agtcggtgcc ggccggactt cgccccgtta
     1741 gatcaagccg cggcggcgtt tggaggtttg cggaaccggc ttcatgcgag aagccgtaac
     1801 cggatccacc gtctgtgaga gaagcttgcg acgcgggtag cgtgtaacaa gccgctcgaa
     1861 aatcgcgccg tagctcatgc ctggatcaac catgcgtgga tccagcagat acagatgccg
     1921 atcgctcaag ccgggccgac gcaccagccg gcccatggcc tgcttgagct ggagagcgca
     1981 gcgcatgatc tcggacatga agccgtcggc gcgttccatc ctggcgaggg acaccgaact
     2041 tttttcggta ttgaacggcg cgcgcacgat gaccaggtgg gtgaggatac gatcctgctc
     2101 tggcgcgacg gatgcgtcgg gatgcgcgag attgagcccc gtccaggcgg agccggttgc
     2161 gaaccacagc ggtcgctgcc cgtccatgga caggcgaatg aaacgctccc tcgggacttc
     2221 cagcgacacg ccttcagcct ggtagatggc gttgtcgcca aatttgccgc tggctttcat
     2281 ggccgcgatg aatgcgtagg acgtggccag caccagcatc ccgccgcggt cctgttcttg
     2341 gctgatggat tcgatcacgc tggcaatttc catgtgatac cgggctagat cggcttcgtc
     2401 gggctcggtg ccggaaggag ggcaccagcg atgggcgcgg ctttctccgc tgcggatttc
     2461 aggcgtgtaa agcgtgacgg gttcggtcac ccatgccgga gtgatggggc ggtgcgtcgc
     2521 caggcgttcg cgcgggacgt tgagcttgct gcgcatgtag tcggcgctag ggacgccggc
     2581 gctggagtcg atgtagagcg tcgcggaaac ggcggcgccg gcggcgacgc ggcgccacat
     2641 gaaatccatc ggggcattca cgtgcctggg cccgaccagg acggtgggcc agcggcgata
     2701 tggggtgaac tccacctgca cgagcatgtg ctcgtttgcg tggacggcgc gcttgagcgc
     2761 gtcgacatcg gagagcagct ggcgctcctc atccgtggaa ttgtccttga tgcttttgac
     2821 gatgagctgg ccagcggcga gcagctccgc caccagttct gcgccgcggc cggccgcggg
     2881 ctgacttgtg tgcttggaaa tggaaaggcg tgaatccgac caccgtgaca gctgctcgag
     2941 ccgttcaaag catttcttcg ctgtcgatgc cgcctcgcgg cgcgttgggg atttggatga
     3001 gttggccagc ttgtcgatcc tgcgcaccat catgcgcacg gaaactgaag cggacgccgc
     3061 ttcggcgaat gcgcgctcca gcaagtgcgc ttcgtcgacc agcagtacct cgaagttggg
     3121 caggacgccg cggtcggctt cgaaggagat caacttctcg ttggaagcga tgtaccacgg
     3181 ttgttcggtt ttcggctgat cgcgctcgcc actgcccgcg cccgccgcca gagcagagcg
     3241 gacccgcgcc agtgtgtcgt gggcaagcat ggcgtgggat gcaaaaacaa tgggcgcttt
     3301 ttccgcagcg acgaactggc gctgatacgc catctcaccg ggctcgccca cctcggcgtt
     3361 gtgaagggcg tattcacgag caaggcccgg gcaatgggct tcgagttcgg ccgacagcca
     3421 ggtggcattg tcgccagcgg cgatttgccg atcgagccac ttgcgcgctt cggcgatctg
     3481 ggcgtgcccc aagtcagctc gcgcctgcgc ttcgtcaagc atctccgaga ggcggcggct
     3541 tgagacaaac tcacggcgcc cgtacaccac ggcaaatttc acttcgccgt gggagcggtt
     3601 gatggcagcc aggtccgagc gcgtctggcg ggaaatcaag tgcgtcggtg aagcgacaac
     3661 gacgcgcttg ccggcgctgg ccgccatgtg cgcggccgcg gcgatcacca agcctttgcc
     3721 gacgcccgtg gcggcctccg cgaggatgat ccggcccacc gaattcgctt cggcgatgtc
     3781 agcgaagaat tggagttgct cggggcgcgc cgtgcgctct tgggtcgaga gcagggttgg
     3841 atccaccttg aatggcgtcg ccggcttgat ggcaaccacc gtgtccccgc gcagggcggt
     3901 gatgatcaga cgggatagaa gagtgccgtc ggcaaggtcc ttcagtccct tggccttggc
     3961 gatctcccgc agcctgtcga gtttgtcgcc cggcacgcgc accggtgcgt ggtgcaagac
     4021 ctcgtactgg ggaaccggaa ggatggggcg gtccttcgtg ccttcctcga tggccagact
     4081 caaagcaacc ctggaaaccg ccccctttgc cttgagcaat tcgagatcgg ccaagggcag
     4141 gctgatggtg cgagtcacga tgctgttggt cggcattcag aaatgttagc attttctgaa
     4201 atattataat attcttaaat aatcatgatt caaaacgaag tgatgttctg gatgccggat
     4261 ggccgaccct tgtaccccca ggaggggaga cggcttctgg gcgaactgtg cggcgacaag
     4321 ctggatccag cgttcttcca tcaccaggag gaagaccggc tgttgcccaa gagtggcggc
     4381 gtgccccctg ttcgcttcag cggaaacggg aaaatcttcc gcattattgg tgttggggtt
     4441 gtcggcgcgg aactggtgaa tgagagcttc gcgaccgtac ttgcggcctt gacaaagcgt
     4501 ttcggcccgc tgaaaacgca gatggccgag tccgagcacg ccctgacctt cagcgcgatg
     4561 ccgcggcgct acgcaatccg tcacatgttc ctgtacaagg gcatcgacga gcgcgacaag
     4621 tttgcggcgc tggatgcggt ggccaagcgt gcgtggttgc tggacatggt gtgccgcggg
     4681 cttgcgcggc agtacgaaga actgggctac gacgggaaga tgcccgatct tcgtccccag
     4741 ctgaacgcgt tcgtcgagca cagcgtccat ccgatcaagg atggttcgaa aatccatggc
     4801 ggggttgccg acctggtggt gacgctccag gccaagctgg tggggcattg ggcggccggc
     4861 ttgctcatct cgaagggcta cggtcggctc gatccgtatc tcgaatcgac agatccccag
     4921 gaattcctga tgtcgcggtt cgcggcgaag atggccagcc gatgacgatc cagcacgcaa
     4981 cagagctggt gtgcgaggtg gcaggcctca aacccctgta cacctcgaag gcggaggccg
     5041 cggggcattg cgcctggtgc gcgcgccaca tcgagcaggg tgcgcgctgc tcgaccttta
     5101 ttgcgcctgt caccttcaac gactggccga ttctgggttg cctgacctct tcgatgttgt
     5161 gcggctggtg ccgccgcgtg caggaacaag gcaagccgtt catgcagacg tatggccgcg
     5221 cagtggtgtc gcgctccggg ttcttcgcgt tcgcgtcgaa caacgatcgc gcggcgtttt
     5281 tcgtgaaccc gcctaaaccc ccgttcatcg tcgtgaatat cacggcgcag cagcagcaca
     5341 tttactggaa ggctccggtc aacttctcgc gcgacttgtt gcgcattcag ttcggcgatc
     5401 gtctgctcac cattcgccgc cagcgcgcgc tggatgcggc cgctgacatc gtgaattacc
     5461 tgacagaccc ggccaaccac gttgtgcgcc ctggaacgcg aaaggcggtg ccgcctcgtt
     5521 tcccgatttc catcgaccgt gaccttgagg cgcccaactg cggcgaggtt tcccccgatg
     5581 tgccgcctgc cctgcggcag acgctgggct cattgaccac aggcgagctg tttgccctga
     5641 cctgcatcgt tcccctgcgc ggcgcgacgc cggcgccggc caagctgctc gaactcaaat
     5701 tgaaaggaga gtgacccatg aagcttttga ttgagggtgt gttcaccctg aagacgcccc
     5761 tgcatgttgc gcatgcggga atggaaggtt ccgttgaaag cgggcagatc aggcggtatc
     5821 gccagggcgc ggtgccgctg ccgctggtgc cgacccaaca gattccggtg ttttcggaac
     5881 tggccggcca ggacgcggct accggcgggc gcggcgtgcg gttgcctctg gtgccgatct
     5941 acccgggatc ggcactgcgc ggtcgcatcc acgcggctgc gggcaacgac ctgattcacg
     6001 atctgctgcg ccgctcgttg cagctgacgc gcgaggagta ccacgtgctg cgcgtcaaca
     6061 ccttcagcgg cgccggcgcg cgcgtcgagc cgaccgtaag cggcgtgagc gctgcgcaga
     6121 acgacctgta tgccgcggtg ttcggcggcg ggccgatgat gctgcgcagc ctgctggtga
     6181 ccaacgattt gttcccgatc atctcggaca cggtgagcag cggcctgatc gcggacaagt
     6241 tcgtgcgcca gatcgaccgc gaccttcagg tgcgcggcga gaacatcacg ttctaccagc
     6301 ccagcgtgcg gcgcgacgcg ctgctcgagt tcagcgatcc gctggcgccg atgatcgtga
     6361 aggaatacga gcctgcactc ctggagtggc agacgctctg ctccgagtcc aacgcggccc
     6421 gcaaggcgga gcgtgagtcg gcccagcaga agcgcgcggc caagaaggcg gggatgaagg
     6481 tggcgagcga agcacccgcg ctggacccgg tggcgccggc ggcagctgcg gggggtgaag
     6541 cggccaaggc gaagaaggtt tcgctgaaga accagcaggc catcgaggcg gtagctcctg
     6601 gtgtgcgctt ctatgtccgc gtgggcctgc gcgaaggggc tcgcagcgaa cacgtgggaa
     6661 tgatcaccgc ggcacttggc gcggtgattg aagccaacga cctgggcggg tacagccgta
     6721 acggttacgg tcgcttttcg gcggcgctcg atgtgacggt gaacgatgcg actttcgaga
     6781 acgcgatcac cttcaacggc aatggcctca agatcggcca ggaattgaaa ccgcacctgg
     6841 aggcgtaccg cacggtgctg cagtcctaca cgccggagca attcgcgcgc gcctactcgc
     6901 tggggaacag tcccgatgcc agcgcgtgag gagccgctgc gggtgacgtt tgatctgctg
     6961 acggcggtag ccgcgccgga gcatccgatc cacctcgacg ccctgctctg cggggtgctg
     7021 gaattgcgcg cgcaggccca ggggcgaaag gtcggggatg aggtagaacg acttccgctg
     7081 gcggcccacg ccagcgggtg ctggaaggcc agtgcgctgg tgttcgatgt gcaggagcgc
     7141 ttcaaccgct tcgcgacgcg gcgcgttgac caggatcgcc tggtgaggga catcgcatca
     7201 ggggcggtag gcgggcgcgg cgacgctttg aatttcaaca gtgggccgtt tcgcaactat
     7261 ctgatgacga tcccggtgat ttacgcacgg agcgcgacgg cctggtgcat cggtgatgcg
     7321 gatgaaattc gcacgatgct gctggagttg ccttcgatcg gaaggctggg tcgcaacggc
     7381 tatggcgcca tcggtggtga acctgtggtc gagcacgacg agctcgcgca ccgcctttgg
     7441 gaatcgcgga tcctgcctta cgaagttgca gacgcagacg aagtttttgc gaccgtgcgt
     7501 tatccgtact tcgacaaaac gaagcaaaaa aaagcgtgga taccacgtac gctttcggct
     7561 tctctggtgt gattccattc gccgaatcgc gctctagttc tttcaaaaca aaggtttcta
     7621 taaatgcagg tttactcacc ccgcctttgg tggggtgttc aaaccatcag cttgcgaaat
     7681 accttgagta cagcggttta ctcaccccgc ctttggtggg gtgttcaaac
//
LOCUS       DASZZC010000495         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_618060,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000495 DASZZC010000000
VERSION     DASZZC010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618060"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VHE37_10505"
     CDS             complement(<1..701)
                     /locus_tag="VHE37_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT36009.1"
                     /translation="MRILLTIADLAMASGGPTQSATRTAQELRALGADSAIACGVRAG
                     VSAAADGVELLPIASSAGSVSRALQAPRYRRQLLQRARQWAPDVIYDFGLWLPQNAAS
                     AAAARRLGRPWICSPRGMLEPWSLGSGALRKKIAWRLYQRRLLEGAAALVATSEEERR
                     HLQQLLPRAPVWLVPNGVDLPPPGSAPRRRQAVFLSRLHPKKQPELLIRLWARLRPAG
                     WRLVLAGPADDDYRAS"
     gene            complement(701..1249)
                     /locus_tag="VHE37_10510"
     CDS             complement(701..1249)
                     /locus_tag="VHE37_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36010.1"
                     /translation="MPLDIQANRQARKYSRRELALRVLWACAWPLYRASPRLLYGWRN
                     LLLRLFGARVGRHVQIHPRARVYFPWNLTIGDESALGEDVLVYNLGPVTLGRQVTVSH
                     GAQLCAGTHDYSRADLPLLKPPVNIGDGAWICTEAFVGPGVTVGAGAVVGARAVAVRD
                     VTPWSIVAGNPARAVGTRTLAA"
     gene            1380..>1994
                     /locus_tag="VHE37_10515"
     CDS             1380..>1994
                     /locus_tag="VHE37_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVT36011.1"
                     /translation="MSSRSHLWRLDAVRGFAAFYVFLSHARPLGPVALYQYGQEAVIL
                     FFLLSGFVIDYSTFSGGRALVVRTYLIHRVRRIYPLLLVSLLLGYLSACVAAQGWAPP
                     RLFSLVMNLLMFQDVAHLKRGVFANTYYGVTPLWSLAYEWWFYLLFIPMCLRLRTGDA
                     ARTVVAGTISAIAFVLYQAWPNQPCLYLSYFIIWWCGVSLAREYR"
BASE COUNT          256 a          726 c          713 g          299 t
ORIGIN      
        1 gacgcacggt agtcatcgtc ggctggcccg gccaaaacca gccgccagcc ggcgggccgc
       61 aaccgcgccc acaggcggat cagcagttcg ggctgcttct tcggatgcag ccgcgagagg
      121 aacacggcct ggcgccggcg cggcgcggag cccggcggcg gcaggtcgac gccgttgggc
      181 accagccaca ccggcgcgcg cggcagcagt tgctgcaggt gccggcgttc ttcctcgctg
      241 gtggcgacca gcgcggcggc cccttcgagc aggcggcgct ggtacaggcg ccaggcgatt
      301 ttcttgcgca gcgcgccgct gcccagcgac cacggctcga gcatgccgcg cgggctgcag
      361 atccacggcc ggcccaggcg gcgcgccgcg gccgcgctgg ccgcgttctg cggcagccac
      421 aggccgaagt cgtagatcac gtcgggcgcc cactgccgcg cgcgctgcag cagctggcgc
      481 cggtagcgcg gcgcctgcag cgcgcgcgaa acggaaccgg cggacgaggc gatcggcagc
      541 agctccacgc catcggccgc agccgacacg cccgcgcgca cgccgcaggc gatggcgctg
      601 tccgcgccca gcgcgcgcaa ctcctgcgcg gtgcgcgtgg ccgactgcgt gggtccgccg
      661 gatgccatgg cgagatcggc gatggtcagc aggatgcgca tcaggccgcg agcgtgcgcg
      721 tgcccaccgc gcgcgccggg ttgccggcga cgatgctcca cggcgtcacg tcgcgcaccg
      781 ccaccgcgcg cgcgcccacc accgcgccgg cgccgacggt gacgccggga ccgacgaagg
      841 cctcggtgca gatccaggcg ccgtcgccga tgttcaccgg cggtttcagc agcggcaggt
      901 cggcgcggga gtagtcgtgc gttcccgcgc acagctgcgc accgtgcgac accgtgacct
      961 ggcggccgag cgtgaccggt cccaggttgt agaccagcac gtcctcgccc agcgcgctct
     1021 cgtcgccgat ggtgaggttc cacgggaagt acacgcgcgc gcgcggatgg atctgcacgt
     1081 gccggcccac gcgcgcgccg aacaggcgca gcagcaggtt gcgccagccg tagagcaggc
     1141 gcggactggc gcggtacagc ggccaggcgc aggcccagag cacgcgcagg gccagctcgc
     1201 gccggctgta cttgcgcgcc tgtcggttgg cctggatgtc gagtggcatg cgatggagat
     1261 cccggcgaaa gtttagcgcg cctgcgcgcg ccggttgccg ccgccggtgg cggcagacta
     1321 cattccgcgc cgtcgatgtg ccttatcccc ttgccttccc gcctgcgccg gaccgccgct
     1381 tgagcagccg cagccacctg tggcgactgg acgcggtgcg cggcttcgcc gcgttctacg
     1441 tgttcctcag ccatgcgcgg ccgctgggcc cggtggcgct gtaccagtac ggccaggaag
     1501 cggtgatcct gttcttcctg ctgtcgggct tcgtcatcga ctactcgacc ttctccggcg
     1561 gccgcgcgct ggtggtgcgt acctacctga tccaccgcgt gcggcgcatc tacccgctgc
     1621 tgctggtgtc gctgctgctg ggatacctga gcgcctgcgt ggccgcgcag ggctgggcgc
     1681 cgccgcgcct gttcagcctg gtgatgaacc tgctgatgtt ccaggacgtg gcgcacctca
     1741 agcgcggcgt gttcgccaac acctattacg gcgtcacgcc gctgtggtcg ctggcctatg
     1801 aatggtggtt ctacctgctg ttcattccca tgtgcctgcg cctgcgcacc ggcgacgcgg
     1861 cgcgcaccgt ggtggccggg acgatctcgg ccatcgcctt cgtgctgtac caggcctggc
     1921 ccaaccagcc ctgcctgtac ctgtcgtact tcatcatctg gtggtgcggc gtatcgctgg
     1981 cgcgcgagta ccgc
//
LOCUS       DASZZC010000496         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_555200,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000496 DASZZC010000000
VERSION     DASZZC010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555200"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>1733
                     /locus_tag="VHE37_10520"
     CDS             17..>1733
                     /locus_tag="VHE37_10520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HVT36012.1"
                     /translation="MFDTMQLREDYRFFLRAFDCFEQERADRKLRTFADLIYDPVRAL
                     NLDKSQIRHIENKFSHVIIDEYQDVNEIQQTMMRWITGTRAEVMVIGDPSQCIYVWRG
                     AKPEYIVSGFERDFGACKTYQLSRTFRYGHRISNLANHCINQNRDRPDALCLSAPSTP
                     DSKITCLDESQGIGPIIDAWRQQGRKLEEIAVLPRLFSMAAGIEMELLDKGTPYRLVG
                     SESILESPITNAVLGFMHLCHKTLAQHPRLPALVKAMLTVPPLGITQGEIKEAGYKII
                     NAPQLASQVLTQMAAFAKPFQAGNLRKRAQIFNLFASISPDAKAETAIKQFASESGAV
                     AFFSKSPTAEAADEKAKIIEALVFYAKKGDLTIRQFIAAIAKMEQAMQKLEDADDALL
                     ITSIHRAKGLEWPVVFIPGLKDGSFPFFSAERPMTRRAIEDERRLYYVGVTRAKEHLY
                     LMHPRDASLAQAMTAGAQTHPEHPLASRFLFESNISLSSAVGTALANPSTFVFRPAEH
                     PGVANRYMKELGCALEIPQTPPAVTVALRQVPPATPPPSVVYDGSRFALGDRVEHPKF
                     GSGTVR"
BASE COUNT          346 a          609 c          495 g          283 t
ORIGIN      
        1 gatggagccg gtcgacatgt tcgacaccat gcagctgcgc gaggactacc gtttcttcct
       61 gcgcgcgttc gactgcttcg agcaggagcg cgccgaccgc aagctgcgca cctttgccga
      121 tctgatctac gacccggtgc gcgcgctcaa tctcgacaag agccagattc ggcatatcga
      181 aaacaagttc tcccacgtca tcatcgatga gtaccaagac gtgaatgaaa tccagcaaac
      241 gatgatgcgc tggatcaccg gcacgcgggc cgaagtgatg gtcattggag atccgtcgca
      301 gtgcatctat gtgtggcgcg gagccaagcc cgaatacatc gtcagcggct tcgagcgcga
      361 tttcggcgcc tgcaagacct atcagctctc gcgcacgttc cgttacggcc accgcatctc
      421 caacctggcc aaccactgca tcaaccagaa ccgcgaccgc ccggacgcct tgtgcctgtc
      481 ggctccgtcg accccggaca gcaagatcac ttgcctggat gagtcccagg ggatcggccc
      541 tatcatcgac gcctggaggc agcaaggccg caagctggaa gaaattgcgg tgcttccgcg
      601 tctgttctcc atggccgcgg gcatcgagat ggagctcctc gacaagggca cgccgtatcg
      661 cctggtcggc tccgagtcga tcctggaatc gcccatcacc aatgccgtgc tcggctttat
      721 gcacctgtgc cacaagacgc tcgcacagca cccgcgcctg cccgccctgg tcaaggccat
      781 gctgaccgtt ccgccgctgg gcatcaccca gggcgagatc aaggaggcgg gctacaagat
      841 catcaacgcc ccgcagctcg caagccaggt actgacgcag atggccgcgt tcgccaagcc
      901 gttccaggcc ggcaacctgc gcaagcgcgc ccaaatcttc aacctgttcg ccagcatctc
      961 ccccgacgcc aaagccgaaa cggccatcaa gcagttcgcc agcgaaagcg gcgcggtggc
     1021 gttcttcagc aagagtccca cggcggaagc cgcggacgaa aaagccaaaa tcatcgaggc
     1081 gctggtgttc tacgccaaaa agggcgacct caccatccgc cagttcatcg ccgccatcgc
     1141 gaagatggag caggcgatgc agaagctcga agatgccgac gatgcgctgc tcatcaccag
     1201 catccaccgc gccaagggcc tggagtggcc agtcgtattc atccccggcc tgaaggacgg
     1261 ctcgtttccg ttcttctccg ccgagcgccc catgaccagg cgcgcgattg aagacgagcg
     1321 gcgcctgtac tacgtcggcg tcacccgcgc caaggagcac ctgtacctga tgcacccacg
     1381 cgacgcgtcg ctcgcccaag cgatgaccgc cggtgcccag acccatccag aacacccgct
     1441 ggccagccgc ttcctgttcg agagcaacat cagcctgtcc agcgccgtcg gcaccgcgct
     1501 ggccaacccc tcgacctttg tctttcgccc ggcggaacac cccggcgtgg ccaatcgcta
     1561 catgaaggag ctgggctgcg cgctggagat tccccagacg ccgcccgcgg tgaccgtcgc
     1621 tttgcgccag gtgccgccgg cgacaccacc acccagcgtt gtctatgacg gcagccgctt
     1681 cgctctgggc gatcgagtcg aacacccgaa gttcggctcc ggcacggtgc gcg
//
LOCUS       DASZZC010000497         6722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_866529,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000497 DASZZC010000000
VERSION     DASZZC010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866529"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VHE37_10525"
     CDS             complement(<1..562)
                     /locus_tag="VHE37_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVT36013.1"
                     /translation="MRSITRVVRLGSGFALAVAAACAVAQSVDATRVQTLSGWVQGSV
                     TADGAALRWLGVPYAAPPVGALRWRAPQPAAAWSTTLPADHFGSPCTQIGGPYGPPPA
                     GKNWGVDNLGTFGKPVGSEDCLTLNLWRPSGTETRLPVLVFIHGGSNIVGWSGDPIYD
                     GAHLAAAAHALVVTVNYRLGLMGWFTH"
     gene            complement(562..3045)
                     /locus_tag="VHE37_10530"
     CDS             complement(562..3045)
                     /locus_tag="VHE37_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36014.1"
                     /translation="MCPTLKPLARLVAALCVLALPAAWAQDTAAADRSADANQEATAP
                     VASPAAADSATQPEAAPAASQGTASSAAAAAPEPDTIPVKTASEAQPAEAKHESNSGT
                     ATRLEDVVVTATKREESVRKLAETVNTLSGDDLEEMGARELSDYLRYIPGITMQEGDT
                     NINRSISIRGLGPQPGGNPTTGVLIENVSTGDPYQSYLIPDLDPFDLHDLEVLKGPQG
                     TLFGAAALNGAIRYVLNKPELENWSVRAFGSYSWVKYGQSDPTLGAMLNMPIGSTAAL
                     RGVVVRQKQPGLYDDINVNKNEVDADYGSKTMYRVLGRWEPTERLKLNAFYLKQDGYR
                     NDLSIANNDQGQFTRTDTPGQSTSQQKFDVANIDARYQFDWATLIGEASHLNKKQKVF
                     YDGSAIVEPLAEHGIQTLGLNNLAESTSNGYELRLASPDGGDSPWVWLGGLYYNKYKV
                     NASLNTPVANTQFLSQVLTALGFLNLPPNPVNDLVPTPDGLSVEYIKFDPFDANEKAV
                     FGEITRKFFDSRLSVNVGGRYYQETLGAQEDVQGALAQFGQIIGATGHHSMKSKGFNP
                     KASVTYQWTPDIMHYVTVARGFQFGGLNGPAPIPTDNVYPLSYKPSTIWSYETGVRTD
                     WFEKTLQADLTFFYIDWTDMQLRQNTPSGNTDYIDNVGKARSMGLESAVRWLTPVPGV
                     MLVNAASYIRATVQSPYTTSDGVDIHKGDDLPASPHLQSSTSLMYNLPLWSYTAGASL
                     TYSYIGKAWSNVQHDDIIYDYGTVDANLNFAAPNMTSAPELSVGCNNVLDARGVVGKR
                     EIHLQGATLAALTNYSRPRTFTLRLTAHF"
     gene            complement(3087..4400)
                     /locus_tag="VHE37_10535"
     CDS             complement(3087..4400)
                     /locus_tag="VHE37_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT36015.1"
                     /translation="MNIKQATIVVMLVALAGCASQPLEKQQKPLVDLAGKAGATHLKA
                     KRLDDGGLLLNGQLDGNDFSVAIPWQWNRQAVVFANGYRSPGLPTEIPGNPLENDTLG
                     FYRDAYAQGFATGLSAYAKAGMAVEHGATATLRLKQFVQAVGAQRTYLMGGSMGGNIT
                     VALLEQHPDDFAGAISFCGAVGGWRRLIGWAIDVRATYNYFTRGTGYELPGEPSIAQS
                     ALWLPQTSWPKEISLPILMWQFRRINKPVQALFDAAKANPAGPERRIIDNIVAVTGAA
                     NDPAGLLMPLLLVTTGQDDFNTTFGGSIYDNTDKVYSSPLLSADENRALNAGIERIKS
                     DPAAVAYAEKWHEPTGELRTKLLTIYNQVDPLVPQAINEKPLREAVAHAGHADLLAQR
                     EVPSKHAPMPMSKLEGLTHCGFEPEQIAAAWADLRAWVENGRKPQ"
     gene            complement(4418..6586)
                     /locus_tag="VHE37_10540"
     CDS             complement(4418..6586)
                     /locus_tag="VHE37_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT36016.1"
                     /translation="MRRATSILSIFASALWAAGAAHAAPDADARAAATEAQMSDDERF
                     HLLLTNLPLKMEPGKQPTLIPGAPITPGFVTGIPRLGIPDILETDASLGVVNPYGLRE
                     GDVATALPSTLAIASSFDPDLAYRAGAMIGSEARAKNFNVLLAPGVNLTRDPRNGRNF
                     EYLGEDPLLAGTLAGESARGIQSNGVVATIKHFALNAQETLRHSLNARIGEAALRESD
                     LLAFEIGIERGQPGSVMCAYNQVNGAYSCGSDFLLNQVLKRDWGYPGWVMSDWGAVHE
                     VGYFNAGLDQHSGAQIDKQLWFDQPLKNEYAAGRVSKARLSDAVRRILRSIYAVGADW
                     SPKKSDIDYDAHGKVAREVEASGIVLLKNEGVLPLDGSVKSVAVIGGYAIAGVMSGGG
                     SSQVMPVGGPAAILPQEVHHIVQRFGGQLIVPSSPEAALEQNLTGVRVSYDSGYDIGN
                     AAAAAARSNVAIVFATKWQTEGGDSSTLNLPGDQDALIAAVAKANPNTVVVLETGNPV
                     LMPWLPQVKAVVEAWYPGQKGGNAIADVLTGAVNPSGRLPVTFPASEQQNPRPQLPGQ
                     GRPDDGSELNVDYPEGSDVGYRWFAAQQQKPLFAFGHGLSYTTFEQGELRLSAGTQVG
                     ATLRVKNTGKRDGADVAQLYLVNAAGRPLQRLAAFQRVPLKAGESKKVNLVLDPRVLA
                     DWVDGGWRIKAGTYQFALGRSAAELGTVVSVQLGEQRLKP"
BASE COUNT         1073 a         2170 c         2232 g         1247 t
ORIGIN      
        1 catgggtgaa ccagcccatc aggcccagcc ggtagttgac cgtcaccacc agcgcgtgcg
       61 ccgcggcggc gaggtgcgca ccgtcgtaga tgggatcgcc cgaccagccg acgatgttgg
      121 agccaccatg gatgaaaacc agcaccggca gcctggtctc agtgccgctg ggccgccaca
      181 gattcagcgt caggcaatcc tcgctgccca ccggcttgcc gaaggtgccg aggttgtcca
      241 cgccccaatt tttgcccgcg ggcggaggac cataagggcc gccgatctgc gtgcagggcg
      301 aaccaaaatg atcggccggc agtgtcgtgc tccaggctgc cgccggctgc ggcgcgcgcc
      361 agcgcaatgc gcccaccggc ggcgcggcgt atggaacgcc gagccagcgc agtgctgcac
      421 catcggcggt gacggaaccc tgcacccagc ccgacagcgt ctgcacgcgc gtcgcatcca
      481 ccgactgcgc gacggcgcag gcggccgcaa cggccagcgc gaagccgctg cccaggcgca
      541 ccacgcgcgt gatgctgcgc atcagaagtg ggccgtcagc cgcagcgtga aggtgcgagg
      601 acgcgagtag ttggtgagtg ccgccagggt cgcgccctgc agatggattt cgcgcttgcc
      661 caccacgccg cgcgcatcga gcacgttgtt gcagccgaca ctcagttccg gcgccgaagt
      721 catgttcggc gcggcgaagt tgaggttggc gtcgaccgtg ccatagtcat agatgatgtc
      781 gtcgtgctgc acgttggacc aggccttgcc gatgtaggaa taggtcaggc tggcgcccgc
      841 ggtatacgac cagagcggca ggttgtacat cagcgaggtg gaactctgca ggtgcggcga
      901 ggccggcagg tcatcgccct tgtggatgtc gacgccgtcg gaagtggtgt acggcgactg
      961 caccgtggca cggatatacg aggccgcatt gaccagcatc acgcccggaa ccggcgtcaa
     1021 ccagcgcacc gcgctttcca gccccatgct gcgcgccttg cccacgttgt cgatgtagtc
     1081 ggtgttgccg ctgggcgtgt tctgccgcag ctgcatgtcg gtccagtcga tgtagaagaa
     1141 cgtgagatcg gcctgcagcg ttttctcgaa ccaatccgtg cgcacgccgg tttcgtagct
     1201 ccagatggtg ctgggcttgt agctcagcgg atagacgttg tcggtgggga tcggcgcggg
     1261 gccgttgagg ccgccgaact ggaagccgcg ggccacggtg acgtaatgca tgatgtccgg
     1321 cgtccactgg taggtgacgc tggccttggg gttgaagccc ttggacttca tcgagtggtg
     1381 gccggtggcg ccgatgatct ggccgaactg cgccagcgcg ccctgcacat cctcctgcgc
     1441 accgagggtt tcctggtagt agcggccacc cacgttgacc gacaggcggc tgtcgaagaa
     1501 cttgcgcgtg atttcgccga acacggcctt ttcgttggcg tcgaagggat cgaacttgat
     1561 gtattccacc gacaggccgt ctggcgtcgg caccaggtcg ttcaccgggt tgggcggaag
     1621 gttgaggaac cccagcgcgg tgagcacctg cgacaggaac tgcgtattgg ccaccggcgt
     1681 gttgagcgac gcgttgacct tgtacttgtt gtagtaaagg ccaccgagcc agacccaggg
     1741 gctgtcgccg ccgtcgggtg acgccagccg cagctcgtag ccgttggaag tgctttccgc
     1801 caggttgttg aggcccagcg tctggattcc atgctcggcc agcggttcga cgatggcgct
     1861 gccgtcgtag aacaccttct gcttcttgtt caggtgcgac gcttcgccga tcagcgtcgc
     1921 ccagtcgaac tggtagcgcg catcgatgtt ggccacgtcg aacttctgct gggaagtgct
     1981 ctgccccggt gtgtcggtgc gggtgaattg cccctgatcg ttgttggcaa ttgaaaggtc
     2041 gttgcggtag ccgtcctgct tgagatagaa ggcattgagt ttcaaccgtt cggtcggctc
     2101 ccagcggcca agcacgcggt acatggtctt gctgccgtaa tcggcatcga cctcgttctt
     2161 gttgacgttg atgtcgtcgt acaggcccgg ctgtttctgc cgcaccacca cgccgcgcag
     2221 cgccgccgtg ctgccgatgg gcatgttcag catggcgccc agcgtgggat cgctctgccc
     2281 gtacttcacc cagctgtagc tgccaaacgc gcgcaccgac cagttttcca gctcaggctt
     2341 gttcagaaca tagcggatgg cgccgttgag cgcggccgcg ccgaacagcg tgccctgcgg
     2401 tcccttgagc acttccaggt cgtgcaggtc gaacggatcg aggtcgggaa tcaggtagct
     2461 ctggtacggg tcgccggtgg agacgttctc gatcagcaca ccggtggtcg ggttgccgcc
     2521 gggctgcggg ccgagaccac ggatcgaaat ggagcggttg atgttggtgt cgccctcctg
     2581 catggtgatg ccggggatgt agcgcaggta atccgacagt tcgcgcgcgc ccatctcttc
     2641 caggtcgtcg cccgacagcg tgttgaccgt ttcggcgagc ttgcgcactg attcctcgcg
     2701 cttggtggcc gtgacgacca cgtcttccag ccgcgtggcg gttccgctgt tgctttcgtg
     2761 tttcgcctcg gctggctgcg cctcgcttgc tgtcttcacc gggatggtgt cgggctccgg
     2821 cgcagcagcg gcagcggacg acgcggtgcc ctggctcgcc gcaggtgcgg cttccggttg
     2881 cgtagcggag tcggccgcag ccggtgaggc aactggcgcc gtggcttcct ggtttgcgtc
     2941 ggcggatctg tctgctgccg ccgtgtcctg cgcccacgcg gccggcagag ccagcacgca
     3001 gagcgcggca accaggcgtg ccagcggttt cagtgttggg cacatcgatt tttctcccct
     3061 caaattcatc ttccggcgca accctttcac tgcggcttgc gtccgttttc aacccacgca
     3121 cgcagatcgg cccaggccgc ggcgatctgc tccggctcga agccgcagtg ggtcaggcct
     3181 tcgagcttcg acatcggcat cggtgcgtgc ttggacggca cttcgcgctg cgccagcaga
     3241 tccgcatgcc cggcgtgggc cacagcttcg cgcaacggct tttcgttgat ggcttgtggt
     3301 accagaggat cgacctggtt gtagatagtc agcagcttgg tgcgcagttc accggtcggc
     3361 tcgtgccact tttccgcata ggccaccgcg gcaggatctg acttgatgcg ctcgatgccg
     3421 gcattcagcg cgcggttctc gtcggccgac agcagtgggc tggaataaac cttatccgtg
     3481 ttgtcataga tcgagccgcc aaaggtggtg ttgaaatcgt cctgccccgt cgtcaccagc
     3541 agcaagggca tcagcaagcc cgccgggtca ttggccgctc cggtaacggc aacgatgttg
     3601 tcgatgatgc ggcgctccgg gccggccggg ttggccttgg ccgcgtcgaa cagggcctgc
     3661 accggcttgt tgatgcgccg aaactgccac atcaggatcg gcagggaaat ctccttgggc
     3721 cacgacgtct gcggcagcca cagcgccgat tgcgcgatgc tgggctcgcc tggcaattcg
     3781 tagcccgtgc cgcgggtgaa gtagttgtaa gtcgcgcgca catcgatggc ccagccgatc
     3841 agccggcgcc agccgcccac ggcaccgcaa aaggaaatag cgcccgcaaa atcgtctgga
     3901 tgctgttcga gcagcgcgac ggtgatgttg ccgcccatcg agccgcccat caggtaggtg
     3961 cgctgcgcgc ccacggcctg gacgaattgc ttcagccgca gcgtggcggt tgcgccatgc
     4021 tcgaccgcca tgccggcctt ggcgtacgcg gacaggccgg tggcgaaacc ttgcgcatag
     4081 gcatctcgat agaaaccgag tgtgtcgttt tccagcggat tgccgggaat ctccgtcggc
     4141 agccccggcg agcggtaacc gttggcgaat accaccgcct gccggttcca ctgccaggga
     4201 atggcgacgg aaaaatcgtt gccgtcgagc tggccgttga gcagcagccc gccgtcgtcc
     4261 agccgtttcg ccttgaggtg cgtagcaccg gccttcccgg ccaggtcgac cagcggcttc
     4321 tgctgcttct ccagcggttg cgacgcacag cccgccagcg cgacgagcat caccacgatt
     4381 gttgcctgct tgatgttcat gctgctctcg cttccgatta cggtttcagg cgctgctcgc
     4441 cgagttgcac gctgaccacg gtgccgagtt cggcagcgga tcggcccagg gcgaactggt
     4501 aggtgccggc cttgatgcgc catccgccgt cgacccaatc cgcgagcaca cgcggatcga
     4561 gcaccaggtt caccttcttc gattcaccgg ccttcaaggg cacgcgctgg aatgcagcca
     4621 ggcgctgcag cggcctgccg gcggcgttca ccaggtacag ctgcgccacg tcggcgccat
     4681 cgcgcttgcc ggtgttcttc acgcgcaagg tcgcgccgac ctgcgtgccc gcagacagcc
     4741 tgagttcgcc ttgctcaaag gtcgtgtagc tgagtccgtg gccgaaggcg aacagcggct
     4801 tctgctgctg cgccgcgaac cagcggtagc cgacgtcgga gccttccggg taatccacgt
     4861 tcagctcgga accatcatcc ggccggccct ggcccggcaa ttgcggccgc gggttctgct
     4921 gctcgcttgc ggggaaagtc acgggcaggc ggccggacgg attgaccgcg ccggtcagca
     4981 cgtcggcgat tgcgttgcca cccttctgtc ccggatacca ggcctcgacc accgctttca
     5041 cctgcggcag ccacggcatc agcaccgggt tgccggtttc cagcaccacg accgtattgg
     5101 gattggcctt ggccaccgcg gcgatcagcg catcctggtc gccggggaga ttcagcgtgc
     5161 tgctgtcgcc accctcggtc tgccacttgg tggcaaacac gattgcaacg ttcgatcgcg
     5221 cagccgccgc ggctgcattg ccgatgtcat aaccgctgtc gtaactcacc cgcacgccgg
     5281 tgaggttctg ctcgagcgca gcctcgggcg acgagggcac gatcagctgg ccgccgaagc
     5341 gctgcacgat gtgatgcact tcctgcggca ggatcgccgc cggcccgccg accggcatca
     5401 cctggctgga gccgccgcca ctcatgacgc cggcaatggc atagccgccg atgacggcca
     5461 ccgatttgac cgacccgtcg agcggcagca cgccctcgtt cttcagcagc acgatgccgg
     5521 aggcttccac ctcgcgcgcc accttgccgt gggcgtcgta gtcgatatcg cttttcttcg
     5581 gcgaccagtc cgcgcccacc gcgtagatcg agcgcaggat gcggcgcacc gcatccgaca
     5641 ggcgcgcttt cgacacgcgg ccggcggcgt actcattctt cagcggctgg tcgaaccaca
     5701 gctgcttgtc gatctgcgcg ccggaatgct ggtcgagacc ggcgttgaaa tagcctactt
     5761 cgtgcacggc gccccagtcg gacatgaccc agcccgggta accccagtcg cgcttgagca
     5821 cctggttgag caggaaatcg ctgccgcagg aatacgcgcc gttaacctgg ttgtaggcgc
     5881 acatcaccga accgggctgg ccgcgctcga tgccgatctc gaaagccagc aggtcggatt
     5941 cgcgcagtgc ggcttcgccg atcctcgcat tgagcgaatg acgcagcgtc tcctgcgcgt
     6001 tgagcgcgaa atgcttgatg gtggcgacaa cgccgttgct ctggatgccg cgcgcggact
     6061 cgccggccag cgtgccggcc agcagcgggt cttcacccag gtactcgaag ttgcggccgt
     6121 tgcgcggatc gcgcgtgagg ttcacgccgg gcgccagcag cacgttgaag ttcttggcgc
     6181 gcgcttcgct gccgatcatc gcgccggcgc ggtaggccag atcgggatcg aagctggacg
     6241 cgatggccag cgtcgagggc aaggcagtgg ccacatcgcc ctcgcgcagg ccataaggat
     6301 tgaccacgcc gaggctggca tcggtttcga ggatgtccgg gatgccgagg cgcgggatgc
     6361 cggtgacgaa ccccggcgtg atcggcgccc cgggaatcag cgtgggctgt ttgccgggct
     6421 ccatcttcag cggcaggttg gtcaacagca gatggaagcg ctcgtcgtcg ctcatctgcg
     6481 cttcggtcgc ggcggcgcgt gcatccgcgt cgggcgcggc gtgcgcggca ccggccgccc
     6541 acaaggccga tgcgaagatg gaaagaatgg atgtcgctcg tctcactgat gtgtctcccc
     6601 gaaaatttgt accggcccca gcaggccgga cggcagcagc ggcgtgtcgt gccggtacgg
     6661 cagcgacacc agcccaggca cgtagtagtt gaaggtggaa aaggcgtaag gctgcacgcc
     6721 gg
//
LOCUS       DASZZC010000498         8336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_866533,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000498 DASZZC010000000
VERSION     DASZZC010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866533"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VHE37_10545"
     CDS             complement(<1..223)
                     /locus_tag="VHE37_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36017.1"
                     /translation="MKTQAVVVADDPVYLNWLQNSASGAEFTLVRPVDAEDLIERVQM
                     MGRVDMVFFQVDAATVAARVALIERLLDRM"
     gene            complement(227..1567)
                     /locus_tag="VHE37_10550"
     CDS             complement(227..1567)
                     /locus_tag="VHE37_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HVT36018.1"
                     /translation="MKSKDRLFSVACLLAALLLPWTASAAVPDANILLVEKGTHRLFR
                     ESGSVKRVAVGDPTVADVNVINRRELLITGKALGITSLIVWPSSGSTTHEYRIKVGPI
                     QDPSTVQKVDKDLVQAQIETGRSVDGVLPNLLAHRRAKLDAINGGKDPVTDRSEVALD
                     TQVMTEVKIAEVSRTTAQQFGLNAFKNTASTAAGMAAPGALSSVVSASSGIGYQFNQS
                     LPILNAFNIVVGNPNKGLLGVLSVLESKGMARTLAEPTLLAQSGQTASYLAGGEFPVP
                     VPQSGVSAGGITIDYKEFGVKLKLTPTVLNRDRIALKVAPEVSDLDFSAGIQVGGIAV
                     PALTIRRTDTSIELGDGESFVISGLISSNLLNNVDKVPWLGSLPVLGAFFRETKINKK
                     ELELIMVVTPHLVRPMGREARLPKLPGAKYDNYDPSFAETMLLETGDFETGFSK"
     gene            complement(1600..2511)
                     /gene="cpaB"
                     /locus_tag="VHE37_10555"
     CDS             complement(1600..2511)
                     /gene="cpaB"
                     /locus_tag="VHE37_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVT36019.1"
                     /translation="MSSTALKLIAVGMVLLAVILGAVTINVYRQNAQKAQDAAVKAEQ
                     EKNSPQVVAMVAVKPLAAYKAIDRESVALVPVAVAPTQYFTSFDEVVGRTPLVDIDAG
                     APITSRYFKEGNVLARVIPPGYQAVSLEINDVIAVGGFVRPGDIVDVLLFLRAGSGVD
                     QPQSRVLLKEARLLAYEDRIIDRPQGLRDDEKTGTQQQRRTRTAVLAVPEAETTRVML
                     GASLGEVRLALRSQGDAETAKAEAGVPLSNETVAAREAQKVPDQTVTASQLSAVREAP
                     AVAQKKAAPAPSVEVFRGSEHENISRN"
     gene            complement(2546..3034)
                     /locus_tag="VHE37_10560"
     CDS             complement(2546..3034)
                     /locus_tag="VHE37_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HVT36020.1"
                     /translation="MTVKAGRKAQSGAAAVEFAFVFPILFLLMYGVVVYAYTFVLQES
                     ISFAAQEAAEAAVAVDPHAANPDALRDARVRAAAVAILNWLPAAQKSRVTGTSGEQVQ
                     IQYCAAGSGGLCPPDTDGVIVTLHFNMTTPDNLFPVLSMPLIGTVPPLPTQLTAQAVA
                     RV"
     gene            complement(3042..3497)
                     /locus_tag="VHE37_10565"
     CDS             complement(3042..3497)
                     /locus_tag="VHE37_10565"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HVT36021.1"
                     /translation="MPQTAASIALGLWAGSVAWFDFTRRRIPNALLILALVPAVLAVG
                     INGRGLLGAGVVASAQGLLVGGLPLLPGYAMGQMGAGDVKFSACIGLLLGPRGALEML
                     LVAAVALGLTSAGVLLASRGGLRRHRIATGPMLAGAFGVQLLWGQLLLS"
     gene            complement(3503..3679)
                     /locus_tag="VHE37_10570"
     CDS             complement(3503..3679)
                     /locus_tag="VHE37_10570"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVT36022.1"
                     /translation="MLKKVMNFLRDEEGASAVEYGLIIGLIAVALIVVLTLLSGGLEG
                     LFNTAATATTNASK"
     gene            3848..4681
                     /locus_tag="VHE37_10575"
     CDS             3848..4681
                     /locus_tag="VHE37_10575"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HVT36023.1"
                     /translation="MTVYAIGDLQGCLDPLQSLLAKLDFNVRRDRLLLTGDLVNRGPQ
                     SLEVLRFVRSLGKAATTVLGNHDLHLLAAAESGKFGPRDTLDSIMAAPDRDELLGWLR
                     RQPLAHEDAETGALLLHAGVAPQWTRAQTLACAAEASSYLNSVNGDRFLRKMYGNEPD
                     RWDENLGGALRLRFIVNCLTRLRYCRVDGSIDLKHKGPPGSQPDGLLPWFEVPGRKTA
                     RDTIICGHWSSLGRVHWPQARVYGLDTGCIWGNCLTALNLLTGELRSVACTQFPKQHG
                     D"
     gene            complement(4728..7583)
                     /locus_tag="VHE37_10580"
     CDS             complement(4728..7583)
                     /locus_tag="VHE37_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVT36024.1"
                     /translation="MSDTPNQELTTTQKILRAIEPLIYARRGLTMGILLIATAFLLWQ
                     MLQIRTDAGYDKSIPLEHEYMKVLKQYQADFGGANNVLVALIQKPGKGEIYNEKFLTS
                     LKSATDEVFFLPGVDRSRVSSLFTPDVRFIEVVEGGFKGGNVIPAEYAPTPEMFALVK
                     ANVNKGGHVGRYTTKDQTGAMIFSELLEVDPVTGEKLDYANVAALLEDRIRGRFTSPK
                     KYVYTLKEDYVTPDGKYHFKAGDTVDEGFVDLGWKKAITSFNAVKKEEGGDKAEIVPI
                     NGRLLDVHEADNPQYNPDIDVHILGFAKVVGDIIDAIFLVVLFFVVTLIMTGLLLWAY
                     LGSFKLSLLPLLCSVTAVIWEFGLLRMAGKGLDPFAILVPFLILAVSVSHGVQYCNAW
                     VGEITQNKRDSYHASLETWRRLAVYGTMAIMTDVVGFALIGLIPIDIIREMALNATLG
                     MLAIIITNKVMMPIWLTWIDIGDPVAFAAKQEKRDSVFDPLWRVISHMVRPVPATIAI
                     VIAAALLGWGLWKGQGLQVGDSQAGVPELLPDSRYNNDTAAVTSNFTIGTDVLKVIAE
                     MEPESCVKFETMDQIDRFYWHMDNTAGVQSTISLPWAAKQVNTAFSEAAPKFKVLPRN
                     QYTMVQAITPLPTSSGLLNPDCSALAVFVFTKDHRAATLERIVDTVKQYNKDNAAEYY
                     ETHKDVNAEYCASKVQARREAGSKKVELQKYIEKLKKKNHELTEDDINKDAGVVKLTK
                     EADDTKDAFKKMDKECPVNFALASAQVGVMAATNEEVHRLERPILYIVYLGIIICVYL
                     SFFEWQSIVAIMLPLALVSFIAYAIMTILDIGMKVATLPVVALAAGIGVDYGIYVYAT
                     FADACAAGYKMEEGYFKTLKLTGKAVVFTGITLCLGVLTWKFSGLQFQRDMGALLVFM
                     FGANMFGAILLLPAIASFILKPKVLKEGEVPVFKPRH"
     gene            complement(7621..>8336)
                     /locus_tag="VHE37_10585"
     CDS             complement(7621..>8336)
                     /locus_tag="VHE37_10585"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HVT36025.1"
                     /translation="VGHDAVILHTADGGKTWKLQNFQPELEKPLLSVLCTDAKTCFAV
                     GAYGLFKKTVDGEHWTDVDAPAIRADELHFNQIARLHDGSLFIAGEQGMLGHSTDGGN
                     TWTKITSPYDASLFGALPVGEKGALIFGLRGNAYVTQDVAKAQWTKIETGTVSSFFGG
                     TTLPNGELALVGLNGVLLVTDANGGNVHTVKVTFKEIDKNGQQTSNELSSTLSAAIPF
                     GGGMVLVGEQGVQNLASVQ"
BASE COUNT         1427 a         2754 c         2607 g         1548 t
ORIGIN      
        1 gcatgcggtc gagcaggcgc tcgatcagcg ccacgcgcgc ggcgacggtg gcggcatcca
       61 cctggaagaa caccatgtcg acgcggccca tcatctgcac gcgctcgatc aggtcctcgg
      121 catccaccgg ccgcaccagt gtgaactcgg cgccgctggc cgagttctgc agccagttca
      181 ggtataccgg atcgtcggcg acgactacgg cctgggtttt catggcctat ttcgaaaatc
      241 cggtttcgaa gtcgcccgtc tccagcagca tggtttcggc gaagctggga tcgtagttgt
      301 cgtacttcgc gcccggcagc ttgggcagcc gcgcctcgcg tcccatcggc cgcaccaggt
      361 gcggcgtcac caccatgatc agttcaagct ccttcttgtt gatcttggtt tcgcggaaga
      421 atgcgccgag caccggcagg ctgcccagcc acggcacctt gtcgacgttg ttgagcaggt
      481 tgctgctgat cagcccggag atgacgaaac tctcgccgtc acccagctcg atgctggtat
      541 cggtgcggcg gatggtcagc gccggtaccg cgatgccgcc gacctggatg ccggcggaga
      601 aatcgagatc ggacacttcc ggagccacct tcagcgcgat gcggtcgcga ttgagcacgg
      661 tgggcgtgag cttcagtttc acgccgaatt ccttgtagtc gatggtgatg ccgccggccg
      721 acacgccgct ctgcggcacc ggcacaggaa attcgccgcc cgcgaggtaa ctggccgtct
      781 ggccgctctg cgccagcagc gtgggctccg ccagggtgcg cgccatgccc ttcgactcga
      841 gcacgctgag cacaccgagc aggcccttgt tcggattgcc gaccacgatg ttgaaagcgt
      901 tgaggatcgg cagcgactgg ttgaattgat agccgatgcc ggaactggcg gataccacgc
      961 tggacagcgc gccgggagcg gccatgccgg cagccgtgct ggcggtgttc ttgaaggcat
     1021 tgaggccgaa ctgctgggca gtggtccggc tcacttcggc aatcttcacc tcggtcatca
     1081 cctgggtgtc gagcgcgacc tcgctgcgat ccgtcaccgg gtccttgccg ccgttgatcg
     1141 cgtcgagctt ggcgcggcga tgcgccagca ggttgggcag cacgccgtcc acgctgcgcc
     1201 cggtttcgat ctgcgcctgc accagatcct tgtcgacctt ctgcaccgtc gaagggtcct
     1261 ggatcggccc gaccttgatg cgatactcgt gcgtcgtact gccgctgctc ggccacacga
     1321 tcaggctggt gatgcccagc gccttgccgg tgatcagcag ttcgcgccgg ttgatcacgt
     1381 tgacatcggc cacggtggga tcgcccactg ccacgcgctt gaccgacccg ctttcgcgga
     1441 acagccggtg ggtgcccttc tcgaccagca ggatgttggc gtccggcacc gcagcggacg
     1501 ccgtccacgg cagcagcagg gctgccagca aacaggccac gctgaaaaga cgatccttcg
     1561 atttcatgag ggtgcctgct gctttcttat tggtgtggtt cagttacgcg agatgttttc
     1621 gtgctcggaa ccgcggaaca cttcgactga tggtgccggg gccgccttct tctgcgccac
     1681 cgccggggcc tcgcgtaccg ccgacagctg gctggccgtc accgtctggt ccgggacctt
     1741 ctgcgcttcg cgcgccgcaa cggtctcgtt ggacagcggc acgccggcct cagccttggc
     1801 tgtctcggcg tcgccctggc tgcgcagcgc cagccgcact tcgcccaggc tggcaccgag
     1861 catcacgcgc gtggtttctg cttccggcac ggcgagcacg gccgtgcgcg tgcggcgctg
     1921 ctgttgcgtg ccggtcttct cgtcatcgcg cagaccttgc ggacggtcga tgatgcggtc
     1981 ttcgtaggcc agcaggcgcg cttccttcaa cagcacgcgc gactgcggct ggtcgacacc
     2041 cgagccggcg cgcaggaaca gcagcacatc gacgatatcg cccgggcgga caaaaccgcc
     2101 gacggcaatg acgtcgttga tctccagcga caccgcctga tacccgggcg ggatcacccg
     2161 cgccagcacg ttgccttcct tgaaatagcg cgaggtgatc ggtgcgccgg catcgatatc
     2221 caccagcggc gtgcgcccga cgacttcgtc gaaactggtg aagtactgtg tgggtgccac
     2281 ggccaccggg accagtgcca ccgactcgcg gtcgatggcc ttgtatgccg ccagcggctt
     2341 gaccgccacc atggccacga cctgcgggga attcttctcc tgctcagcct tgaccgccgc
     2401 atcctgcgcc ttctgcgcat tctggcggta cacgttgatg gtcactgcgc cgagtatcac
     2461 cgccagcagc accatgccta ccgcgatcaa tttaagcgcc gtgctgctca tcctgactcc
     2521 ctatccaatt cccttttgcc ccggctcaga cgcgggcgac tgcctgcgcc gtgagctgcg
     2581 tcggtaacgg tggaaccgta ccgatcaacg gcatgctgag caccgggaaa agattgtccg
     2641 gtgtggtcat gttgaaatgc aaggtgacga tgacgccatc ggtgtcgggc gggcacaagc
     2701 caccgctgcc ggctgcgcaa tactggatct gcacctgctc gcccgacgtg ccggtcacgc
     2761 gactcttctg cgccgccggc agccagttga ggatggccac cgcggcagcg cgcacgcgcg
     2821 cgtcgcgcag cgcatcggga ttggccgcat gcgggtcgac ggccaccgcc gcctccgccg
     2881 cttcctgcgc cgcgaagctg atcgactcct gcagcacgaa ggtataggcg tagacgacca
     2941 cgccgtacat cagcaggaac aggatcggga agacgaaggc gaattcgact gcggccgccc
     3001 ccgactgcgc cttgcgcccg gctttgacag tcatgctcat gtcacgacag cagcagctgc
     3061 ccccataaca gttgcacccc gaaagcaccg gccagcatcg gccccgtggc gatgcgatgg
     3121 cggcgcaggc cgccccgcga agccagcagc acaccggcgc tggtcagacc cagcgccacc
     3181 gcggccacca gcaacatctc caaggctccg cgcggtccga gcagcaggcc gatacaggca
     3241 gagaacttga cgtcgccggc gcccatctgg cccattgcgt accccggcag cagcggcagg
     3301 ccgccaacca gcaatccctg ggcacttgcc acaacacccg caccgagcaa cccgcgcccg
     3361 ttgatgccga cggcgagtac cgccggcacc agggcgagta tcagcagggc gttgggtatg
     3421 cgccggcgcg tgaaatcaaa ccaagccacc gagcccgccc acaaccccag agcgatgctg
     3481 gcagcggtct gcggcactgg ccttatttcg acgcgttggt ggtcgcggtg gccgccgtgt
     3541 tgaacaaccc ttccaggccg cccgacagca gcgtgaggac gacgatcagc gcaacggcga
     3601 tcaggccgat gatcaggccg tattcgaccg cgctggcgcc ttcctcatca cgcaggaaat
     3661 tcatgacttt cttgagcatt tcgacactcc taaaggctgg ttgcggcccg tggatgccgc
     3721 gtgaagtact atgcgtttgg gtccgggaca agtcaaagct tccggcgagc cggaacggta
     3781 ccgctcatcg gctctgaatt gtccgctttg gtgcacgctt ccatctgaaa atactgatgt
     3841 tccgctcatg accgtctatg ccatcggcga tctgcagggt tgcctcgacc cgctccagag
     3901 cctgctggcg aaactggatt tcaacgtccg gcgcgatcgc ctgctgctga ccggcgacct
     3961 ggtcaaccgc ggaccgcagt cgctcgaagt gctgcgcttc gtgcgcagcc tgggcaaggc
     4021 cgcgaccact gtcctcggca atcatgacct gcacctgctc gcggctgcgg agagcggaaa
     4081 gtttggtccg cgcgacacgc tcgattcgat catggcggcg ccggatcgcg acgaattgct
     4141 gggctggctg cgtcggcagc cgctcgcgca cgaagatgcg gagaccggcg cgctcctgct
     4201 gcatgcaggc gtggcgccgc aatggacgcg ggcacaaacg ctggcttgcg ccgccgaggc
     4261 aagcagctat ctgaacagcg tcaacggcga ccgcttcctg cgcaagatgt acggcaacga
     4321 acccgatcgc tgggatgaaa atctgggcgg agcgctgcgc ctgcgcttca tcgtcaactg
     4381 cctcacgcgc ctgcgctact gccgtgtcga tggcagcatc gatctcaagc acaagggccc
     4441 gccaggcagc cagccggacg gactgctgcc ctggttcgaa gtgccgggcc gcaagaccgc
     4501 gcgcgacacc atcatctgcg gacactggtc ctcgctcggc cgcgtgcatt ggccgcaggc
     4561 gcgcgtgtac ggcctggaca ccggctgcat ctggggcaac tgcctcaccg cgctcaacct
     4621 gctcaccggc gaactgcgca gcgtcgcctg cacgcagttc cccaagcaac acggcgactg
     4681 aaaaaaagaa accccgccgt agcggggttt cctgatacgc aggcggttca gtgcctgggc
     4741 ttgaacacgg gcacttcgcc ttccttcagc actttcggct tgagaatgaa cgaggcaatg
     4801 gccggcagca gcaggatggc accgaacatg ttggcgccga acatgaacac cagcagggca
     4861 cccatgtcac gctggaactg caggccggag aacttccagg tgagcacgcc caggcacagc
     4921 gtgatgccgg tgaacaccac cgccttgccg gtgagcttga gggtcttgaa gtagccctct
     4981 tccatcttgt agcccgcggc gcaggcatcg gcaaacgtcg cgtagacgta aatgccgtag
     5041 tccacgccaa tgcccgccgc cagcgcaacc accggcaagg tggccacctt catgccgatg
     5101 tcgaggatgg tcatgatggc gtaggcgatg aacgacacca gcgccagcgg cagcatgatg
     5161 gcgacgatcg actgccactc gaagaacgac aggtacacgc agatgatgat gccgaggtag
     5221 acaatgtaca ggatcggccg ctcgaggcga tgcacctcct cgttggtcgc cgccatcacg
     5281 cccacctgcg ccgacgccag cgcgaagttc accgggcatt ccttgtccat cttcttgaat
     5341 gcgtccttgg tgtcgtcagc ttccttggtc agcttgacca cgccggcatc cttgttgatg
     5401 tcgtcctcgg tcagctcgtg gttcttcttc ttcagcttct cgatgtactt ctgcaattcg
     5461 actttcttcg agcccgcctc gcgccgggcc tggaccttgg atgcgcagta ctcggcattc
     5521 acatccttgt gtgtctcgta atactcggcg gcattgtcct tgttgtattg cttcacggtg
     5581 tcgacgatgc gctcgagcgt cgccgcgcgg tgatccttgg tgaacacgaa gacggccagt
     5641 gccgagcagt cgggattgag caggcccgaa gaggtgggca gcggcgtgat cgcctgcacc
     5701 atggtgtact ggttgcgcgg cagcaccttg aacttgggcg cggcctcgga gaacgcggtg
     5761 ttgacctgct tggccgccca cggcaatgaa atggtcgact gcacgccggc ggtgttgtcc
     5821 atgtgccagt agaagcggtc gatctggtcc atggtctcga acttcacgca cgactccggc
     5881 tccatctcgg cgatcacctt gagcacgtcg gtgccgatgg tgaaattgct ggtcaccgct
     5941 gcggtgtcgt tgttgtagcg cgaatccggc agcagttccg gcacgccggc ctggctgtcg
     6001 cccacctgca ggccctggcc cttccacagg ccccagccga gcagcgcggc ggcaatgacg
     6061 atggcgatgg tcgccggcac cggccgcacc atgtgcgaga tcacgcgcca cagcggatcg
     6121 aacacgctgt cgcgcttctc ctgcttggcg gcgaaggcca ccggatcgcc gatgtcgatc
     6181 caggtcagcc agatcggcat catcaccttg ttggtgatga tgatcgccag catgccgagc
     6241 gtggcgttga gcgccatctc gcggatgatg tcgatgggga tcaggccgat cagcgcgaag
     6301 ccgaccacgt cggtcatgat cgccatggtt ccgtacaccg ccagccggcg ccaggtttcc
     6361 aggctcgcgt ggtagctgtc gcgcttgttc tgcgtgattt cgccgaccca ggcattgcag
     6421 tactgcacgc cgtggctaac cgacaccgcc aggatcagga acggcaccag aatggcgaag
     6481 ggatcaagtc ccttgcccgc catgcgtagc aggccgaact cccagatcac cgccgtcacc
     6541 gagcacagca gcggcagcag cgacagcttg aacgaaccca ggtaggccca cagcagcagg
     6601 ccggtcatga tcagggtgac gacgaagaac agtaccacca ggaaaatggc gtcgatgatg
     6661 tcgccgacca ccttggcgaa gcccaggata tggacgtcga tgtccgggtt gtactgcggg
     6721 ttgtccgcct cgtgcacgtc gagcaggcgg ccattgatgg gaacgatctc ggccttgtcg
     6781 ccgccctctt ccttcttcac cgcattgaag ctggtgatgg ccttcttcca gcccaggtcc
     6841 acgaagccct catccaccgt gtcgccggcc ttgaagtggt acttgccgtc gggcgtgacg
     6901 tagtcttcct tgagcgtgta gacgtatttc ttcgggctgg tgaagcggcc acggatgcgg
     6961 tcttccagca gtgcggcgac gttggcgtag tcgagcttct cgccggtcac cgggtcgacc
     7021 tccagcagct cggagaagat cattgcgccg gtctggtctt tcgtcgtgta gcggcccaca
     7081 tggccgccct tgttcacgtt ggccttcacc agcgcgaaca tctccggcgt cggcgcgtac
     7141 tcggccggga tgacgttgcc gcccttgaag ccgccttcca ccacttcgat gaagcgcaca
     7201 tcgggcgtga acagcgagga cacgcgcgaa cggtcgacgc cgggcaggaa gaacacttcg
     7261 tccgtcgcgc tcttcagcga ggtgaggaac ttctcgttgt agatctcgcc cttgcccggc
     7321 ttctggatca gcgccaccag cacattgttg gcgccgccga aatcggcctg gtactgcttg
     7381 agcaccttca tgtactcgtg ctcgagcggg atcgacttgt cgtagccggc gtcggtgcgg
     7441 atctgcagca tctgccacag caggaaggcg gtggcgatga gcaggatgcc catggtcagg
     7501 ccgcgccgcg cgtagatcag cggctcgatg gcgcgcagga tcttctgcgt ggtggtcagt
     7561 tcttgattgg gtgtatcgct catagctcgt ccccggttct tgtacggcgt gtcgctgcgc
     7621 ttactgcacg ctcgccaggt tctgcacgcc ctgctcgccc accagcacca tgccgccgcc
     7681 gaacgggatc gccgccgaca gcgtgctcga cagctcattg ctggtctgct gcccgttctt
     7741 gtcgatttcc ttgaaggtaa cctttaccgt gtgcacgttg ccgccgttgg cgtcggtcac
     7801 cagcagcacg ccattgaggc cgaccagcgc cagttcgccg ttgggcagcg tggtgccgcc
     7861 gaagaacgac gacaccgtgc ccgtctcaat cttggtccat tgcgccttgg ccacgtcctg
     7921 cgtcacgtag gcgttgccgc gcaggccgaa gatcagcgcg cccttttcgc ccaccggcaa
     7981 ggcgccgaac agcgacgcgt cgtacggcga ggtgatcttg gtccatgtgt tgccgccgtc
     8041 ggtcgagtgg ccgagcatgc cctgctcgcc ggcgatgaac aggctgccgt catgcaggcg
     8101 cgcaatctgg ttgaagtgca gttcatcggc gcggatcgcc ggcgcatcga cgtcggtcca
     8161 gtgctcgccg tcgacggtct tcttgaacaa gccataggcg cccaccgcga agcaggtctt
     8221 ggcgtcggtg cacagcaccg acagcaacgg cttctccaac tcgggctgga agttctgcag
     8281 cttccaggtc ttgccgccgt cggcggtatg caggatgacg gcatcgtggc ccaccg
//
LOCUS       DASZZC010000499         7552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1067270,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000499 DASZZC010000000
VERSION     DASZZC010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067270"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /locus_tag="VHE37_10590"
     CDS             complement(<1..1962)
                     /locus_tag="VHE37_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36026.1"
                     /translation="MTIERGLRAALALAGLCALAACHNSAPQNTLAGGTGSPTGSSTG
                     SSTGSSTGSTTGSTGGPLPCSASHAFAAPATEKVALTHYGRRVTPLGHLTDVGNFPGG
                     GALTPDGKFYWAVDTGKDVNYVWIVNVASGNVIQQLPLPGTGGHVVFAPDGKTAYVPG
                     NPMGGSSFPDGTPVKAPAGDAIHVYAVDPATGLAAEQDPIAIPSSANPLVPVGSSRRN
                     SSIIATQSIDAFTFTEWPEDIAISPDGKTLLTALLNSDRAAIINTASKAVTVVTVGTY
                     PRGAAIERSGRFGYISNSNDGTLSKIDLAAGTVSSTIALGLSGDDISDAESGALTLLA
                     DPAADRLYVTVANRDLVMVLDTGSDTFSKRIDLRRYGGQGGLGTTPVGLAESPDGCTL
                     YVANAYDNALISVALKDRPDTATKAYDLVGAIPVGDYPTAVAVTPDGGRLVWLAGKGM
                     GVNKGANGNAPERIKQYWQRGIVGVLDVPDDRYFALQNTTVAANLTADVPAPPAATVV
                     HGAATGPFAYAPSSQMKYVFLVVKENRTYDNIFGTLARGVTDAFGGARYELYEDNCGA
                     SNTAFEGASRTHPGCGVTPNAHALVRRWPLLTQFMANSEQSEEGHVFTAGGWLTDYLQ
                     RNSHWEPSGRGRPYDTGIYPVAYPPKYFIF"
     gene            complement(1952..4906)
                     /locus_tag="VHE37_10595"
     CDS             complement(1952..4906)
                     /locus_tag="VHE37_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36027.1"
                     /translation="MSSRRCIVLAMGLLAGCHHGSPPLDNGGSGTTGSSTGSTTGSTT
                     GGTTGGGTPTARCGANVPANVTEYDVANTGRRMLPLGRMTAVGDFPTGGRLTPDGKFY
                     WSVSAGHGRNDVQIVNVASGAVTQVLPLPGAYGEMVFAPDGRHAYVSGEPKGGSVPSG
                     PTQGDGGDVVHVFSVDPATGLAAEQDPIVLPSTTGGSARQNDFPPDLGVGPLGTLPSF
                     PVGLAVSADGKTLVVVLYNADQVAIVDLTNAAATPALVQVGKYPYGVAIERSGHFAYV
                     SNAYDGTLSKIDLASASVSATVSGLGGPAGDQNSQPQQVIADPLADRVYAAVTNFDGV
                     AVIDTTTDTVSKFISMKRSEGYGSQPVALALSNNSDTLYVADAGENAIAAIALSDRPA
                     GGAKAFDVIGKMPTADYPSDVDVTPDGCTLVWTAGRGLGAGPNPTYGRPLGQLPRVNN
                     DTDANKVPYPTYVPDMLIGRVGVVPAPTDATFAALAPAVDALVTPANAQPPPADTPLH
                     GAKQADGSYAPSDKIKYVFYVVRENRTYDQIYGSETRHGGADGDPTLEVLGDNDENIP
                     RGGPAGPGSGVTPNAHALARMFVLLDRFFEDSEVSVDGHVITAGAYANNYSLKSMHSG
                     YSGRGRPGNDVGVFPISFPPNHFLFDQAAAQGISFHNYGELSGGAAPVFSDDGRSTYL
                     QVLANSDTETYPNNLFIGCFESSQNAPNSPLCAFDAGMTATLGAPLAQSRINVFNATF
                     TVQSLTCTAATLDTPACLVPHFNYLIMPNNHTNGTGAGARDPLAMCADNDLGIGQLVD
                     IISHSAIWPETLIVVVEDDSQDGADHVDAHRAPVLVMGPWVKHLGKDAPAIHTRYDQY
                     SAIRTIELALGMQPLSVFDATATPMYDVFTSTPDNTPYTAIMPQQDIMAVNPASAANA
                     EFSAALPFHKLDAVPQEINDRILWQRVHGAGSLPPRPGPNASRREAQRARDMYSAYYA
                     GRDLKAVSDDDD"
     gene            complement(5077..7221)
                     /locus_tag="VHE37_10600"
     CDS             complement(5077..7221)
                     /locus_tag="VHE37_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36028.1"
                     /translation="MRLKACAAAAALMLPFNPAAAADEPAADDAAPIVLLPMQVRGER
                     EAAPVSDVPSSYTRISGDELRARGATDLRSALALAAGINVAPGGDEGPAGAAPGLLGQ
                     RETDDFMLAIDGVPVGGVFSAPFAAIDLAGVERIEVLRGASPVVLGAGAFAGVINIVH
                     YAAGADGDHVAVGGSTYGSLDGAARVALPALGAWQQSLIASGQRQGYSDPRQNVHREH
                     LLYRGGRSLDAGDVHLDVDALDLRQQPGSPSPDGSSPDVNVNPSDAALEDQRLRVAAG
                     FEQVRGDGRWSATLSASRALHRGVQGFLRNPSAGDSGDALDGLLDPGDDDDDDSGNAA
                     GFRQQRGVTDAYADAHYSRSYDSGLGLLAGADLLYGSAHLQSHGFGYDVDPSGANPPS
                     SAGLADAGYSDVQDQREIAGLYSQLALRLPRRLNLYAGLRANYAHESLDTAALEDGAY
                     ATGRDRRSDTRLGAVAGASWRLLRDDEADDGVLLYADYRGSSQPAQLDFGPEVESRSI
                     LAPETTQNVELGIKSILFDGHVELELSALAAHFDHLVLAQQVNGEPALVNAGAENFRA
                     LQLEGGVALADHLHLTANLSLQQARYDQFLTLDDDGNAIQLQGRELPLSPRQLAALGL
                     VYAPAEGFQAALIGNYRGRRFLDNANTRAAGGYATWEASAGWRMHGWELRVAGENLGN
                     RRDPVALSELGDEQLYLLPARRVRLGLSVDFE"
     gene            7338..>7552
                     /locus_tag="VHE37_10605"
     CDS             7338..>7552
                     /locus_tag="VHE37_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVT36029.1"
                     /translation="MSDDAYNAEAASDIGHLRLDVEPMSPDATVTQAADLFLLPANAG
                     LLSLPVVDDGRVTGSISRHQLNNIFLRR"
BASE COUNT         1110 a         2574 c         2641 g         1227 t
ORIGIN      
        1 gaagatgaaa tatttcggcg gatatgccac cgggtagatg ccggtgtcgt acggccggcc
       61 gcggcccgac ggttcccagt gcgagttgcg ctgcaggtaa tcggtgagcc agccgccggc
      121 ggtgaacacg tggccttctt ccgactgctc gctgttggcc atgaactgcg tgagcagcgg
      181 ccagcgccgc accagcgcgt gcgcgttggg cgtgaccccg cagccgggat gcgtgcggct
      241 ggcgccctcg aacgcggtgt tcgatgcgcc gcagttgtct tcgtacagct cgtacctggc
      301 gccgccgaac gcgtcggtga cgccgcgcgc cagcgtgccg aagatgttgt cgtaggtgcg
      361 gttctccttc accaccagga acacgtactt catctggctg ctcggcgcgt aggcgaacgg
      421 cccggtggcg gcgccgtgca ccacggtggc ggccggcggc gccggcacat ccgcggtgag
      481 gttggcggcc accgtggtgt tctgcagcgc gaagtagcgg tcgtcgggca catcgagcac
      541 gccgacgatg ccgcgctgcc agtactgctt gatgcgctcc ggcgcgttgc cattcgcacc
      601 cttgttcacg cccatgccct tgcccgccag ccacaccagc ctgccgccat cgggcgtcac
      661 cgccaccgcg gtggggtaat cgccgaccgg gatggcgccg accaggtcat aagctttggt
      721 cgcggtgtcg gggcggtctt tcagcgccac cgagatcagc gcgttgtcgt aggcattggc
      781 cacgtacaac gtgcagccat cgggcgattc cgccaggccc accggcgtgg tgcccaaccc
      841 gccctgcccg ccgtagcggc gcaggtcaat gcgcttgctg aaagtgtcgc tgccggtgtc
      901 gagcaccatc accaggtcgc ggttggccac ggtgacatag aggcgatcgg ccgcgggatc
      961 ggccagcagt gtcagcgcgc ccgattccgc atcggaaatg tcatcgccgg aaagacccag
     1021 cgcgatggtg ctgctgaccg tgccggcagc gagatcgatc ttggagagcg tgccgtcgtt
     1081 ggaattggag atgtagccga accttccgct gcgctcgatg gccgcgccgc gcggatacgt
     1141 gcccaccgtc accaccgtca ccgccttgct tgcggtgtta atgatcgcgg cgcggtcgga
     1201 gttgagcagc gcggtgagca gcgtcttgcc gtcgggcgag atggcgatgt cttccggcca
     1261 ctcggtgaag gtgaaggcat cgatgctctg cgtggcaatg atcgagctgt tgcgacgcga
     1321 cgagcccacc ggcaccaggg gattcgcgct cgacgggatg gcgatcgggt cctgctcggc
     1381 ggcgaggccg gtggcgggat cgaccgcgta gacatggatg gcatcgccgg ccggcgcttt
     1441 caccggcgtg ccgtcgggaa agcttgatcc gcccatcggg ttgccgggca cgtaggcggt
     1501 cttgccgtcg ggcgcgaaca ccacgtggcc gccggtgccg ggcagcggca gctgctggat
     1561 cacgttgccg ctggcaacgt tgacgatcca cacgtagttg acgtccttgc cggtgtccac
     1621 cgcccaatag aatttcccgt cgggcgtcaa cgcgccgcca ccgggaaaat tgccgacgtc
     1681 ggtgagatgg cccagcggcg tcacgcgccg gccgtaatgc gtcagtgcta ctttttccgt
     1741 agcaggcgca gcaaaggcgt ggctggccga gcacggcagc ggcccgccgg tgctgccggt
     1801 ggtcgagccg gttgacgatc cggtcgatga gccggtcgaa cttccggtcg gactgccggt
     1861 gccgccagcc agggtgttct gcggcgcgct gttgtggcac gccgccagcg cgcatagccc
     1921 cgcaagcgcc agcgccgcgc gcaagccgcg ttcaatcgtc atcatcggac accgccttca
     1981 gatcccggcc cgcgtaatac gcggaataca tgtcgcgcgc gcgctgcgcc tcgcgccgcg
     2041 aggcgttcgg ccccggccgc ggcggcagcg agccggcgcc gtgcacgcgc tgccacagga
     2101 tgcggtcgtt gatttcctgc ggcaccgcgt cgagcttgtg gaacggcagc gccgcggaga
     2161 actctgcgtt ggccgcgctc gccgggttca ccgccatgat gtcctgctgc ggcatgatcg
     2221 cggtgtaggg cgtgttgtcc ggcgtgctgg tgaagacgtc gtacatcggc gtggcagtgg
     2281 cgtcgaacac cgacagcggc tgcatgccca gcgcgagctc gatggtgcgg atggcgctgt
     2341 actggtcgta gcgcgtgtgg atggccggcg cgtccttgcc caggtgcttg acccacgggc
     2401 ccatcaccag caccggcgcg cggtgcgcgt cgacatggtc ggcaccgtcc tgcgaatcgt
     2461 cctccaccac cacgatcagc gtctccggcc agatggccga gtgcgaaatg atgtcgacca
     2521 gctggccgat gcccaggtcg ttgtccgcgc acatggccag cggatcgcgc gcgccggcac
     2581 cggtgccgtt ggtgtggttg ttcggcatga tcaggtagtt gaagtgcggc accaggcagg
     2641 ccggcgtgtc cagcgtggcc gcggtgcagg tgagcgactg cacggtgaag gtggcgttga
     2701 acacgttgat gcgcgactgc gccagcggcg cgcccagcgt cgccgtcatg ccggcgtcga
     2761 atgcgcacag cggcgagttg ggcgcgttct ggctcgactc gaagcagccg atgaacaggt
     2821 tgttcggata ggtctcggtg tcgctgttgg ccagcacctg caggtaggtg ctgcggccgt
     2881 catcggagaa caccggcgcg gcgccgccgg acagctcgcc gtagttgtga aacgagatgc
     2941 cctgcgcggc cgcctggtcg aacaggaagt ggttgggcgg aaagctgatc gggaacacgc
     3001 cgacgtcgtt gcccgggcgg ccgcggccgg aataaccgga gtgcatgctc ttcagcgagt
     3061 agttgttggc gtaggcgccg gcggtgatga cgtgtccgtc caccgacacc tcgctgtcct
     3121 cgaagaagcg gtcgagcagc acgaacatgc gcgccagcgc gtgcgcgttg ggcgtcacgc
     3181 cgctgcccgg cccggccggt ccgccgcgcg gaatgttctc gtcgttgtcg cccagcacct
     3241 ccagcgtggg atcgccgtcg gcgccgccgt ggcgcgtctc gctgccgtag atctggtcgt
     3301 aggtgcggtt ctcgcgcacc acgtagaaca cgtacttgat cttgtcgctg ggcgcgtagg
     3361 agccgtcggc ctgcttcgcg ccgtgcagcg gcgtgtcggc cggcggcggc tgcgcgttgg
     3421 ccggcgtgac cagcgcgtcg accgcgggcg ccagcgccgc gaaggtcgcg tcggtgggcg
     3481 cgggcaccac gccgacgcgg ccgatcagca tgtcgggcac ataggtcggg tacggcacct
     3541 tgttcgcgtc ggtgtcgttg ttcacgcgcg gcagctgtcc gagcggacgg ccataggtgg
     3601 gattgggccc ggcgccgagc ccgcggccgg cggtccacac cagcgtgcag ccgtcgggcg
     3661 tgacgtcgac atcgctcggg taatcggcgg tgggcatctt gccgatcacg tcgaaggctt
     3721 tcgcgccgcc ggcggggcgg tcgctcagtg cgatggcggc gatggcgttc tcgccggcat
     3781 cggccacgta gagcgtgtcg ctgttgttgg acagcgcgag cgccaccggc tggctgccgt
     3841 aaccttcgct gcgcttcatc gagatgaact tgctcacggt atcggtggtg gtgtcgatca
     3901 ccgccacgcc gtcgaagttg gtcaccgccg catagacgcg atccgccagc ggatcggcga
     3961 tgacctgctg cggctgcgag ttctggtcgc cggccggtcc gccgaggccg ctcaccgtcg
     4021 cactcacgct ggcggaggcg agatcaatct tcgacagcgt gccgtcatac gcattgctca
     4081 cgtaggcgaa gtgcccgctg cgctcgatgg ccacgccgta cgggtacttg cccacctgca
     4141 ccagcgccgg cgtggcggcg gcattggtca ggtcgacgat cgccacctgg tcggcgttgt
     4201 acagcaccac caccagcgtc ttgccgtcgg cgctgacggc aaggcccacg ggaaaactcg
     4261 gcagcgtgcc gagcgggccg acgccgaggt cgggcggaaa atcgttctgc cgcgcgctgc
     4321 cgccggtggt ggaaggcaac acgatcgggt cctgctcggc ggccagcccc gtcgccgggt
     4381 cgacgctgaa cacgtgcacc acgtcgccgc cgtcgccctg ggtcgggccg ctgggcacgc
     4441 tgccgccctt gggttcgccc gacacgtacg cgtgcctgcc atcgggcgcg aacaccatct
     4501 cgccgtacgc accgggcagc ggcagcacct gcgtgaccgc gccgctggcg acgttgacga
     4561 tctgcacgtc gttgcggccg tggccggcgc tcaccgacca atagaatttt ccgtcgggcg
     4621 tgaggcggcc gccggtgggg aaatcgccca ccgcggtcat gcggcccagc ggcagcatgc
     4681 gccggccggt gttggccacg tcgtactcgg tgacattggc cggcacgttg gcgccgcagc
     4741 gcgcggtggg cgtgccgccg ccggtggtgc cgccggtggt tgatccggtc gttgagccgg
     4801 tgctcgaacc ggtggtgccg ctgccgccgt tgtcgagcgg cggactgccg tggtggcagc
     4861 cggccaacaa tcccattgcg aggacgatgc agcgacggct cgacatggcg ttcactccct
     4921 tgcttcgatc atggccgcgc ggcattttcc ggctcgccaa agcccgaccg caacgacatt
     4981 tacactgtta ttaaaaatag tatgaatgta aaaacaataa atattttata acatcgaaaa
     5041 ccggcatgcg gtggaagctt caggcgcggt tttccgctac tcgaaatcca ccgacaggcc
     5101 caggcgcacg cgccgcgccg gcaacaagta cagctgctcg tcgccgagtt ccgacagcgc
     5161 aaccgggtcg cgccggttgc ccaggttctc gccggccacg cgcagctccc agccgtgcat
     5221 gcgccagccc gcgctcgcct cccatgtcgc gtaaccgccg gcggcgcgcg tgttggcgtt
     5281 gtccaggaag cgccgcccgc ggtagttgcc gatcagcgcc gcctggaaac cttccgccgg
     5341 tgcgtagacc agcccgagag cggcgagctg gcgcggcgac aatggcagct cgcgcccctg
     5401 cagctggatg gcgttgccgt cgtcgtcgag cgtgaggaac tggtcgtagc gcgcctgctg
     5461 cagcgacagg ttggcggtca ggtgcaggtg atcagccagc gccacgccgc cttccagctg
     5521 cagcgcgcgg aaattttccg cgccggcgtt gaccagcgcc ggctcgccgt tgacctgctg
     5581 cgccagcacc aggtgatcga aatgcgcggc cagcgccgac agttccagtt ccacgtggcc
     5641 gtcgaacagg atgctcttga tgcccagctc cacgttctgc gtggtctcag gcgccaggat
     5701 gctgcgcgat tccacttccg gcccaaaatc cagctgtgcc ggctgcgaac tgccgcggta
     5761 gtcggcgtag agcagcacgc cgtcgtcggc ctcgtcgtcg cgcaacagcc gccagctggc
     5821 gccggccacc gcgcccaggc gcgtgtcgct gcggcggtcg cggccggtcg cgtacgcgcc
     5881 gtcttcaagc gccgcggtgt cgagcgactc gtgcgcatag ttcgcgcgca ggcccgcgta
     5941 caggttcagg cgccgcggca ggcgcagcgc gagctgcgaa tacaggccgg cgatctcgcg
     6001 ctggtcctgc acgtcgctat agccggcatc cgccaggccc gcgctcgatg gcggattggc
     6061 gccggatgga tcgacgtcgt agccaaaacc atggctctgc aggtgcgcgc tgccatagag
     6121 caggtcggcg ccggcgagca ggcccagacc gctgtcatag ctgcggctgt agtgcgcgtc
     6181 ggcgtaggcg tcggtgacgc cgcgctgctg gcgaaagccg gcggcgttgc cgctgtcgtc
     6241 gtcatcgtca tcgccgggat cgagcaggcc gtcgagcgca tctccgctgt cgcccgcgga
     6301 cgggttgcgc aggaaaccct ggacgccgcg atgcagcgcg cgcgacgccg acagcgtggc
     6361 gctccagcgg ccatcgccgc gcacctgctc gaaacccgcg gcgacgcgca ggcgctggtc
     6421 ttccagcgcg gcatccgacg gattgacgtt cacatcgggc gatgaaccat cgggtgacgg
     6481 actgcccggc tgctggcgca ggtcgagcgc atcgacatcg aggtgcacgt cgccggcatc
     6541 gagcgagcgt ccgccgcggt acagcaggtg ctcgcggtgc acgttctgcc gcggatcgga
     6601 ataaccctgg cgctggccgc tggcgatcag cgactgctgc cacgcaccga gcgccggcag
     6661 cgccacgcgc gccgcgccgt cgagcgaacc gtacgtgctg ccgccgaccg cgacgtgatc
     6721 gccatccgca cccgccgcgt aatgcacgat gttgatcacg ccggcaaacg cgccggcgcc
     6781 caggaccacc ggcgaggcgc cgcgcagcac ctcgatgcgc tcgacgccgg cgaggtcgat
     6841 cgccgcgaac ggcgcgctga acacgccgcc caccggcacg ccgtcgatgg ccagcatgaa
     6901 gtcatcggtc tcgcgctggc cgagcagccc gggcgccgca ccggccggtc cctcgtcacc
     6961 accgggcgcc acgttgatgc cagcggccag cgccagcgcg ctgcgcaggt cggtggcgcc
     7021 gcgcgcgcgc aactcgtcgc cgctgatgcg cgtgtaggaa ctgggcacat cgctcaccgg
     7081 cgcggcttcg cgttcgccgc gcacctgcat cggcagcagc acgatgggcg ccgcatcatc
     7141 cgccgccggc tcgtccgcag cggcagcagg gttgaacggc aacatcagcg cggcggcggc
     7201 cgcgcaggct ttcaggcgca tggattcctc tcccttcccg tccacagttt gcagcaagcc
     7261 ggcatcacgc gctggtcagc gcgtgactcg aatccgcaat caatcgttca tgcgcgccgg
     7321 atagcttccg cacactcatg agcgatgatg cctacaacgc cgaggccgcc tccgacatcg
     7381 gccacctgcg gctggatgtc gaaccgatgt cacccgacgc caccgtgacc caggccgccg
     7441 acctgttcct gctgccggcg aatgccgggc tgctcagcct gccggtggtg gatgacggcc
     7501 gcgtgaccgg cagcatcagc cgccaccagc tcaacaacat cttcctgcgc cg
//
LOCUS       DASZZC010000500         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_362347,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000500 DASZZC010000000
VERSION     DASZZC010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362347"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VHE37_10610"
     CDS             <1..532
                     /locus_tag="VHE37_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT36030.1"
                     /translation="LSTDPAARSLGRAIFLNNCAGCHGADAKGARGFPNLTDHDWLYG
                     GKPDDIVASITHGRNGQMPPFNGAIPADVIDDLVATVTHWSDASLDPAVRERGMKQFA
                     ITCAACHGADGKGNPLLGSANLTDDIWLHGGSKAHVRETILYGRRSAMPAHEKILSAD
                     DIRVVAAYVYGLSQQP"
     gene            529..834
                     /locus_tag="VHE37_10615"
     CDS             529..834
                     /locus_tag="VHE37_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36031.1"
                     /translation="MSEPQHDEALARLAAFVDRRNGGDRRRALPQMPALHYAAVIGWS
                     SFLGAATTLVLLLAVLPELGEGFTLDTLSRSFFVLWLACALTAAYAVMLFRREREPA"
     gene            831..2204
                     /gene="ccoG"
                     /locus_tag="VHE37_10620"
     CDS             831..2204
                     /gene="ccoG"
                     /locus_tag="VHE37_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HVT36032.1"
                     /translation="MSMPSVSLYESAARIQPRMAHGRYARLRVLAMCALLGWYYGLPW
                     LSWNGAPAVLFDLPHRRFNMLMLHLAPQDLYLLSFLLMIAAMTLFLFTSLAGRLWCGY
                     ACPQTVWTEAFLWMERAIEGDRHARLQLDKLPWSSPRKLLRRGARHLAWIVFALYTGA
                     TFVAYFMPARELFPALAHLQLSGWPLFWVLFYGFATWGNAGFMREQVCKYMCPYARFQ
                     SAMFDRHTLIIAYDEKRGEPRKGLSKQLGKSAGSCVNCSLCVQVCPTGIDIRQGLQYE
                     CIACAACVDVCNTVMDAIHQPRGLIRYTSAAREAGGHTRVLRGRTIGYGIVWVVMCTA
                     FLVAIALRSPLDFDVIRDRHSLYRELADERIENVYTVKLGNKDNQPHQLLLSARGAQG
                     AMTIDPSSVTLQPGESRALTVAVRGPAPAEDEHGHGARAEPIKIGVQDADNPKVRRER
                     ETRFFGE"
     gene            2334..>3581
                     /locus_tag="VHE37_10625"
     CDS             2334..>3581
                     /locus_tag="VHE37_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HVT36033.1"
                     /translation="MSAHAPWALYDGPPLLDSISLQRPDGSREALLALPGMHCAACAT
                     RVEQALAGLARDVRINAVTRSVEFSWSPREVRMSQILERLEQAGLQPQALTQDAALAD
                     QAGERRADLRRILIALICSMQVMMLAWPTYEHASEISPAMSMLLRWAQWLLATPAVLY
                     AGAPFLRNAWSALAARSVNMDVPVAGALVIAYGVSAARTVSGHGDVYFDAASMFVFLL
                     SAGRFLEARTQAQAGERLRLLAGARPLTAWRISGERREQVPIVQLRTGDRIAVPAGEA
                     LPVDGELLAHSAELDESLLTGESLPVLRHPGALLLAGSLNAGSAELSCIARRMGADTV
                     LSQITRLLTAAQSERPPVQALVDRVSRHFTSGVLLIAAAAALWWWPQGADRSIEAALA
                     VLVASCPCALALAYPAVLAAASSR"
BASE COUNT          495 a         1311 c         1217 g          558 t
ORIGIN      
        1 gttgtccacc gatccggcgg cacgctcgct gggccgcgcc atcttcctca acaactgcgc
       61 cggctgccat ggcgccgatg ccaagggcgc gcgcggcttt cccaacctga ccgaccacga
      121 ctggctgtac ggcggcaagc ccgacgacat cgtggccagc atcacccacg gccgcaacgg
      181 ccagatgccg ccgttcaacg gcgccattcc cgccgacgtg atcgacgacc tggtggccac
      241 ggtcacgcac tggtcggatg cttcgctcga cccggcggtg cgcgagcgcg gcatgaagca
      301 gttcgccatc acctgcgccg cctgccatgg cgccgacggc aagggcaacc cgctgctcgg
      361 ctcggccaac ctgaccgacg acatctggct gcacggcggc agcaaggcgc atgtgcgcga
      421 gaccatcctg tacggccgca ggagcgcgat gccggcgcac gagaagatcc tctcggctga
      481 cgacatccgc gtggtggcgg cctacgtgta cgggctgtcg cagcagccgt gagcgagccg
      541 cagcacgacg aggcactggc gcggctggca gcgttcgtcg accgccgcaa cggcggcgac
      601 cgccgccgcg cgctgccgca gatgccggcg ctgcactacg cggcagtgat cggctggtcg
      661 agttttctcg gcgccgccac caccctggtg ctgctgctcg ccgtgctgcc cgaactgggt
      721 gaaggcttca ccctcgacac gctgtcgcgc agcttcttcg tgctgtggct ggcctgcgcg
      781 ctcaccgcgg cctacgccgt gatgctgttc cgccgggagc gcgaacccgc atgagcatgc
      841 ccagcgtttc gctgtacgaa tccgccgcgc gcatccagcc gcgcatggcg cacggccgct
      901 acgcgcgcct gcgcgtgctg gccatgtgcg cgctgctcgg ctggtactac ggcctgccct
      961 ggctcagctg gaacggcgcg ccggcggtgc tgttcgacct gccgcaccgg cgcttcaaca
     1021 tgctcatgct gcacctggcg cctcaggacc tttacctgct cagcttcctg ctgatgatcg
     1081 cggccatgac gctgttcctg ttcaccagcc tggccggccg cctgtggtgc ggctatgcct
     1141 gcccacagac ggtatggacc gaggccttcc tgtggatgga gcgcgccatc gagggcgacc
     1201 gccacgcgcg cctgcagctg gacaagctgc cctggagcag tccgcgcaag ctgttgcgcc
     1261 gcggcgcgcg ccatctcgcc tggatcgtgt ttgcgctcta caccggcgcc accttcgttg
     1321 cctatttcat gccggcgcgc gaactgtttc ccgcgctggc ccacctgcag ctgtccggct
     1381 ggccgctgtt ctgggtgctg ttctacggct tcgccacctg gggcaatgcc ggcttcatgc
     1441 gcgaacaggt gtgcaagtac atgtgcccgt acgcgcgctt ccagagcgcc atgttcgatc
     1501 gccacacgct gatcattgcc tacgacgaaa agcgcggcga accgcgcaag ggcctgtcca
     1561 agcagctggg caagtccgcg ggcagttgcg tgaactgctc gctgtgcgtc caggtctgcc
     1621 ccaccggcat cgacatccgt cagggcctgc agtacgagtg catcgcctgc gccgcctgcg
     1681 tcgacgtgtg caacacggtg atggacgcga tccaccagcc gcgcggactg atccgctaca
     1741 ccagcgccgc gcgcgaggcc ggcgggcata cgcgcgtgct gcgcggacgc accataggct
     1801 acggcatcgt ctgggtcgtg atgtgcacgg ccttcctcgt cgccatcgcc ctgcgttcgc
     1861 cgctggactt cgacgtcatc cgcgaccgcc acagcctgta ccgcgaactg gccgacgagc
     1921 gcatcgagaa cgtctatacg gtgaagctgg gcaacaagga caaccagccg caccagctcc
     1981 tgttatcggc acgcggcgca cagggcgcca tgaccatcga tccgtccagc gtgaccttgc
     2041 agccgggcga atcgcgggcg ctcaccgtgg cggtccgcgg cccggcaccg gcggaggatg
     2101 agcacggcca tggcgcgcgc gccgagccga tcaagatcgg cgtgcaggat gccgataacc
     2161 cgaaggtgcg ccgcgagcgc gagacccgct tctttggaga atgaccatga aggcatccac
     2221 atccacgctg ctgctcgacc tgcgcgacgg cgcgctgctg ctgctgttcg ccggcggcct
     2281 gctgctgggc ggcgccgcct tcgcggcgag cttcgtccac tgaaattccg ccgatgagcg
     2341 cccacgcgcc ctgggcgctc tacgacgggc cgccgctgct cgacagcatc agcctgcagc
     2401 ggcccgacgg ctcgcgcgaa gcgctgctgg ccctgcccgg catgcattgc gcggcctgcg
     2461 ccacgcgcgt ggaacaggcg ctggccggcc tggcgcgcga cgtgcgcatc aacgcggtga
     2521 ccaggtcggt ggagttcagc tggtcgccgc gcgaagtgcg catgtcgcag atcctcgagc
     2581 ggctcgagca ggccgggctg cagccgcagg cgctgacgca ggatgccgcg ctggccgacc
     2641 aggccggcga acgccgcgcc gacctgcggc gcatcctcat cgcgctgatc tgctcgatgc
     2701 aggtgatgat gctggcctgg cccacctacg agcacgccag cgaaatcagc ccggcgatgt
     2761 ccatgctgct gcgctgggcg cagtggctgc tggccacgcc ggcggtgctc tacgccggcg
     2821 cgccgttttt gcgcaatgcc tggagtgcgc tggccgcgcg cagcgtgaac atggatgtgc
     2881 cggtggccgg cgcgctggtc atcgcctacg gcgtgtccgc ggcgcgcacg gtgagcggcc
     2941 acggcgacgt gtacttcgat gccgccagca tgttcgtgtt cctgctctcc gccgggcgct
     3001 tcctcgaagc gcgcacgcag gcacaggccg gcgagcgcct gcgcctgctg gccggcgcgc
     3061 gcccgctgac cgcctggcgg atcagcggcg agcgtcgcga gcaggtgccg atcgtgcagc
     3121 tgcgcaccgg cgaccgcatc gcggtgccgg ccggcgaagc actgccggtc gatggcgaac
     3181 tgctggcgca cagcgccgag ctcgacgagt cgctgctcac cggcgaatcg ctgccggtgc
     3241 tgcgtcaccc cggcgcgctg ctgctggccg gcagcctcaa tgccggcagc gcggaactct
     3301 cctgcatcgc gcggcgcatg ggcgccgaca ccgtgctgtc gcagatcacc cgcctgctca
     3361 ccgcggcgca atccgagcgg ccgccggtgc aggcgctggt cgaccgcgtc tcgcggcact
     3421 tcaccagcgg cgtgctgctg atcgccgccg cggccgcgct gtggtggtgg ccgcagggcg
     3481 ccgaccgcag catcgaggcc gcactggcgg tgctggtggc cagctgcccg tgcgcgctgg
     3541 ccctggccta ccccgcggtg ctcgccgccg ccagctcgcg g
//
LOCUS       DASZZC010000501         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_362398,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000501 DASZZC010000000
VERSION     DASZZC010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362398"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VHE37_10630"
     CDS             complement(<1..561)
                     /locus_tag="VHE37_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT36034.1"
                     /translation="MPPPLLQVEQLGIRYAAADGEVSAVAGLSFELAHGEALGMVGES
                     GSGKSQTALAVMGLLAGNARVSGRVTFDGRDLGALAERERNALRGDRMAMVFQDPMTS
                     LNPYLTVGAQMAEVLQQHRGSSRAQALAECARMLDAVKLGDAAARLRAYPHQLSGGQR
                     QRVMLAMALLCRPQLLIADEPTTALDV"
     gene            complement(566..1444)
                     /locus_tag="VHE37_10635"
     CDS             complement(566..1444)
                     /locus_tag="VHE37_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT36035.1"
                     /translation="MTAAIKDAPRGRSLWQDALMRLRANRAAQTAAALLALIVFLVVF
                     GPLLSPYAYDYIDFDGQWAAPPTLRGGHLFGTDGLGRDLFARTLYGGRISLLVGLVAT
                     LVSLLIGVAWGAIAGYFGGRVDQVMMRIVDVLYALPFMFFVILLMVLFGRHLVLIFVA
                     VGAINWLDMARIVRGQTLSLRRREFIDAAVLAGLPAAAIIRRHIVPNLLGVVAVYVTL
                     TLPQVVLTESFLSFLGLGVQEPATSWGALVNDGAREMESSPWSLVFPATLLALTLMCF
                     NFIGDGLRDALDPKDR"
     gene            complement(1441..>1563)
                     /locus_tag="VHE37_10640"
     CDS             complement(1441..>1563)
                     /locus_tag="VHE37_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT36036.1"
                     /translation="LNRDYTLVLGVVIFYGALIVAFNFIVDLLYGALDPRVRLQ"
BASE COUNT          292 a          539 c          524 g          208 t
ORIGIN      
        1 gacgtcgagc gcggtggtgg gttcatcggc gatcaacagc tgcggccggc acagcagcgc
       61 catggccagc atcacgcgct ggcgctggcc gcccgacagc tggtgcggat aggcgcgcag
      121 ccgcgccgcg gcatcgccga gtttcaccgc gtcgagcatg cgcgcacact ccgcgagcgc
      181 ctgcgcacgc gagctgccgc gatgctgctg caggacttcc gccatctggg cgccgaccgt
      241 gaggtatgga ttgaggctgg tcatcgggtc ctggaagacc atcgccatgc gatcgccgcg
      301 cagggcgttt ctctcccgtt cggcaagtgc gccgagatcg cggccgtcga aggtcacccg
      361 tccgctcacc cgcgcgttgc cggcgagcag gcccatgacg gccagggcgg tctgcgattt
      421 gccggagccc gattcgccga ccatgcccag cgcctctccg tgagcgagct cgaagctcaa
      481 gcccgccacc gcgctcacct cgccgtcggc cgccgcgtag cggataccca gttgttccac
      541 ctgcagcaag ggaggcggca tcgtctcagc ggtccttggg atcgagcgcg tcgcgcagtc
      601 catcgccgat gaagttgaag cacatcagcg tcagcgccag cagcgtggcc ggaaagacca
      661 gcgaccacgg cgagctttcc atctcgcgcg caccgtcgtt gaccaatgcg ccccagctgg
      721 tcgccggctc ctgcacaccc agtccgagaa aactcaggaa ggattcggtg agcaccacct
      781 gcggcagcgt cagcgtgaca tagaccgcga ccacgccgag caggttgggc acgatgtgcc
      841 ggcggatgat ggccgccgct ggcagccctg cgagcacggc cgcatcgatg aactcgcggc
      901 ggcgcagcga cagcgtctgg ccgcgcacga tgcgcgccat gtccagccag ttgatcgcgc
      961 ccaccgcgac gaagatcagc actaggtggc gcccgaacag caccatcagc aggatgacga
     1021 aaaacatgaa cggcagcgcg tagagcacgt cgacgatgcg catcatcacc tggtcgacgc
     1081 gtccgccgaa atagccggcg atggcgcccc aggctacgcc gatgagcagt gacaccagcg
     1141 tggcgaccag tcccaccagc aacgagatgc gtccgccgta gagcgtgcgc gcgaacaggt
     1201 cgcggccgag cccgtcggtg ccgaacagat gcccgccgcg cagcgtcggc ggcgccgccc
     1261 actggccgtc gaaatcgatg tagtcatagg cgtagggcga cagcagcggg ccgaagacaa
     1321 ccagaaacac gatcagcgcc agcagcgccg cggcggtttg cgcggcacga ttggcacgca
     1381 ggcgcatgag cgcgtcctgc cacagcgagc gcccacgcgg cgcgtccttg atggcggcag
     1441 tcattgcagg cgcacgcgcg ggtcgagcgc gccgtagagc aggtcgacga taaagttgaa
     1501 ggccacgatc agcgcgccgt agaagatcac cacgccgagc accagcgtgt agtcgcgatt
     1561 gag
//
LOCUS       DASZZC010000502         4801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_362456,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000502 DASZZC010000000
VERSION     DASZZC010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362456"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VHE37_10645"
     CDS             complement(<1..499)
                     /locus_tag="VHE37_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVT36037.1"
                     /translation="MTRRPPAQIKEIPLDLIRPGSQQARRQFNPDTLAELAESIRESG
                     VVQPVVLRTRVWGYELLAGERRWRAAQIAGLREIPAVVRDDLSDSEAFVVGLIENLQR
                     ESLTPMETAAGMKRLAEMYELTHEQVGAKVGKSREYVSNYLRLLNLSPPVQALVNEGH
                     ISLGHA"
     gene            559..1113
                     /gene="folE"
                     /locus_tag="VHE37_10650"
     CDS             559..1113
                     /gene="folE"
                     /locus_tag="VHE37_10650"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HVT36038.1"
                     /translation="MSMEEHFRAIIEAIGENPEREGLLRTPQRAARALEFLTQGYEMD
                     VAEIVNGAIFESANDEMVIVRDIELYSLCEHHMLPFIGKCHVAYLPSGKVIGLSKVPR
                     IVDMYARRLQIQENLTREIGNCIQQVTGAHGVAVVIEAKHLCAMMRGVEKQNSTMITS
                     MMIGRFREDPRTRQEFLTLVKNQA"
     gene            1206..2168
                     /locus_tag="VHE37_10655"
     CDS             1206..2168
                     /locus_tag="VHE37_10655"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HVT36039.1"
                     /translation="MNPDLNQKEVLTSLEPQVRDLMARHKDARKLWMPSDLLPSTDEH
                     EAELAALRERARGLPDSVRVAIALNLLTEEGLPHFHRLVAVYMGTDGVWAEWNNLWTA
                     EEDRHGCVLRDYVRDARIIDMAALERMQYQYVESGFNPEWHGDPYRLLAYTSLQEKAT
                     QTAHSNAGRIAGQYEPKLQRILAHISADESRHYAFYRDSFSAVLKADTNRALAALAAV
                     APALAMPGHTIDGFAQMSEVERRAGIYGPREYMKLVEDLIKHWAIDAMTNLSAMGRAA
                     QDKVMELPKRLTRMAEYIERRAGSRSFSFEFIYQRAFEMAQAKA"
     gene            2168..3001
                     /locus_tag="VHE37_10660"
     CDS             2168..3001
                     /locus_tag="VHE37_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36040.1"
                     /translation="MPLAGQRALITGAGSGLGRALALRLAAHGWRVACSDRDLAAAQA
                     TLALIEKTGGRGLALALDVSRDDAFVEPVAGIAADWGGLDLLVNNAGVATAGTVAEAD
                     LAQWRFAIEINLLGCVRGARAVIPLMSAQGAGHIVNVASFAGIANPPALASYSATKAA
                     VISLSETLRFELAPHGVGVSVACPSFFRTNLMATSQASAPPGAANPAPQMERIVNKLM
                     DTATVTADDVAADIHDAVLKNRFLVITHGDARARYHIKRLAPETFYKLARNATAGFLK
                     K"
     gene            complement(3071..3346)
                     /locus_tag="VHE37_10665"
     CDS             complement(3071..3346)
                     /locus_tag="VHE37_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVT36041.1"
                     /translation="MSNRSYRFIVSGRVQGVFFRQSCAEAARAHQLSGWVRNLTDGRV
                     EGLAHGAEFSLRQLRDWLHHGPPAARVDAVEWIESAEIPDAGFSVRR"
     gene            3365..4048
                     /locus_tag="VHE37_10670"
     CDS             3365..4048
                     /locus_tag="VHE37_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36042.1"
                     /translation="MGILSRARDRIAGALMQRAPLDGPEQMSFAAELPLTGAQAPLWR
                     VQMHIEREPQAGGEKLRVRAHFQANFASALRPALASDPAATDARALPSDARGLTLAQR
                     TGALVQRAAARALQVPFVALVAEPLLQHDFNSWIEIQATTAPLDRGSRDLLPQSEKLA
                     ALGIAPTQRSGDEGPVADSWAGEAPGGFAQVSMLQLDKRHLPPRLAAALGDEPFHLAA
                     TIVNTVEQK"
     gene            4230..>4801
                     /locus_tag="VHE37_10675"
     CDS             4230..>4801
                     /locus_tag="VHE37_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVT36043.1"
                     /translation="MSTPALPLTSAQRKREVLALALPIIGGMVSQNLLNLADAWMVGD
                     LGAAALAATGMANFLNFMAQAFITGFSPAVQAIAARRMGEGRRSEAAVPLNGGLLLSL
                     MIGIPVTFIGVIAAPWIFSALNHDADVVRQGTPYLQWRLVSVAAVGMNFSFRGYWSAA
                     KLAGFYLQTLLLMHVLNVIFSWNLIHGIPAL"
BASE COUNT          817 a         1659 c         1590 g          735 t
ORIGIN      
        1 tggcgtggcc cagcgagata tgcccttcgt tgaccagcgc ctgcaccggc ggcgacaggt
       61 tgagcaggcg caggtagttc gacacgtatt cacgcgactt gccgaccttg gcgccgacct
      121 gttcatgggt gagttcgtac atctccgcca ggcgcttcat gccggcggcg gtttccatcg
      181 gcgtcagcga ctcgcgctgc aggttctcga tcaggccgac gacgaaggcc tcgctgtcgg
      241 acaggtcgtc gcgcaccacc gcgggaatct cgcgcaggcc ggcgatctgc gccgcgcgcc
      301 agcggcgctc gccggccagc agttcgtagc cccacacgcg cgtgcgcagc acgacgggct
      361 gtaccacacc ggattcgcgg atgctctcgg ccagctcggc cagcgtgtcc gggttgaact
      421 ggcggcgcgc ctgctgcgag ccggggcgga tcaggtcgag cggaatttcc ttgatctggg
      481 cgggcgggcg ccgggtcatg acgagggtcg gggaggtttg tgggagaata cgcgccgttt
      541 ttcgagccgg ccgcaacatt gtccatggaa gaacatttcc gcgccatcat cgaagccatc
      601 ggcgagaacc ccgagcgcga aggcctgctg cgcacgccac agcgcgccgc gcgcgcgctc
      661 gaattcctca cccagggcta cgagatggac gtggccgaga tcgtcaacgg cgccatcttc
      721 gagtcggcca acgacgagat ggtgatcgtg cgcgacatcg agctctactc gctgtgcgag
      781 caccacatgc tgcccttcat cggcaagtgc cacgtggcct acctgccgtc gggcaaggtc
      841 atcgggctgt cgaaggtccc gcgcatcgtc gacatgtatg cgcggcgcct gcagatccag
      901 gaaaacctca cccgcgagat cggcaactgc atccagcagg tgaccggcgc gcacggcgtg
      961 gcggtggtca tcgaggcgaa acacctgtgc gccatgatgc gcggcgtgga gaagcagaac
     1021 tcgaccatga tcacctcgat gatgatcggc cgcttccgcg aagacccgcg cacgcgccag
     1081 gaattcctca ccctggtgaa gaaccaggct tgaccctgca tccgggccgc gcttgcggtc
     1141 gtaatgcgcg gcaagccggc ataaaatttg ttgagcctcc gtagaaagcc aaaggacgga
     1201 acgccatgaa ccccgatctg aaccagaagg aagtcctcac ctcgctcgag ccgcaggtgc
     1261 gcgatctgat ggcgcgccac aaggacgcgc gcaagctgtg gatgccttcg gacctgctgc
     1321 cgtcgaccga cgagcacgag gccgagctcg ccgcgctgcg cgaacgcgcc cgcggcctgc
     1381 ccgacagcgt gcgcgtggcc attgcgctca acctgctcac cgaggaaggc ctgccgcact
     1441 tccaccgcct ggtcgcggtg tacatgggca ccgacggcgt gtgggccgag tggaacaacc
     1501 tgtggaccgc cgaggaagac cgccacggct gcgtgctgcg cgactacgtg cgcgacgcgc
     1561 gcatcatcga catggccgcg ctcgagcgca tgcagtacca gtacgtcgag tccggcttca
     1621 accccgagtg gcacggcgat ccgtaccgcc tgctggccta caccagcctg caggagaaag
     1681 ccacgcagac cgcgcactcc aacgccggcc gcatcgccgg acagtacgag cccaagctgc
     1741 agcgcatcct ggcgcatatc tccgccgacg agtcgcgcca ctacgcgttc taccgcgaca
     1801 gcttctccgc cgtgctcaag gccgacacca accgcgcgct ggccgcgctg gctgcggtgg
     1861 cgccggcgct ggccatgccc ggccacacca tcgacggctt cgcgcagatg tcggaagtcg
     1921 agcgccgcgc cggcatctac ggcccgcgcg aatacatgaa gctggtcgaa gacctgatca
     1981 agcactgggc catcgacgcc atgaccaatc tgtcggcgat gggccgcgcc gcgcaggaca
     2041 aggtgatgga actgcccaag cgtcttacgc gcatggccga gtacatcgag cgccgcgccg
     2101 gcagccgcag cttcagcttc gaattcatct accagcgcgc attcgagatg gcgcaggcca
     2161 aggcctgatg ccgctcgccg ggcagcgcgc actcatcacg ggtgcgggat cgggcctcgg
     2221 ccgcgcgctg gccttgcgtc tggctgcgca cggctggcgc gtcgcctgca gcgaccgcga
     2281 cctggctgcg gcgcaggcca cgctggcgct gatcgaaaaa accggcggac gcggactggc
     2341 gctggcgctc gacgtgtcgc gcgacgacgc cttcgtcgag ccggtggccg gcatcgccgc
     2401 cgactggggc gggctcgacc tgctggtgaa caacgccggc gtcgccaccg ccggcaccgt
     2461 ggcggaagcg gacctggcgc aatggcgctt cgccatcgag atcaacctgc tcggctgcgt
     2521 gcgcggcgcg cgcgcggtga tcccgctgat gagcgcgcag ggcgccggcc acatcgtcaa
     2581 cgtcgcttcg ttcgccggca tcgccaatcc gccggcgctg gccagctaca gcgcgaccaa
     2641 ggctgcggtg atctcgctgt cggaaaccct gcgcttcgaa ctggcgccgc acggcgtcgg
     2701 cgtgagcgtg gcctgcccgt cgttcttccg caccaacctg atggccacca gccaggccag
     2761 cgcgccgccc ggcgcggcga acccggcgcc gcagatggag cgcatcgtca acaagctgat
     2821 ggacacggcg acggtgaccg ccgatgacgt ggccgccgac atccacgacg cagtgctgaa
     2881 gaaccgcttc ctggtcatca cccacggcga tgccagggcg cgctaccaca tcaagcggct
     2941 ggcgccggag acgttctaca agctggcgcg caacgccact gcgggcttcc tgaagaagta
     3001 gcttatgtag gagcgcgcct tgcgcgcgat tcacgtctcg atcgcgggca gggttcgctc
     3061 ctgcaaataa tcagcggcgc acgctgaagc cggcatcggg gatctctgcc gactcgatcc
     3121 actcgaccgc gtcgacgcgc gcggctggcg gcccgtggtg cagccagtcg cgcagctggc
     3181 gcagcgaaaa ttccgcccca tgagccaaac cttcgacgcg gccatcggtc agattcctga
     3241 cccagccgct caactggtgc gcacgcgccg cttccgcgca ggactggcgg aagaacacgc
     3301 cctgcacccg gcctgaaacg atgaagcggt atgatctgtt cgacatgaca tcgatgacct
     3361 gttcatggga attctaagcc gcgcgcgtga ccgcattgcc ggcgcgttga tgcagcgcgc
     3421 gccgctcgat gggcccgaac agatgtcgtt cgcggcagag ctgccgctga cgggcgcgca
     3481 ggcgccgctg tggcgcgtgc agatgcacat cgagcgcgag ccgcaggccg gcggcgagaa
     3541 gctgcgcgtg cgcgcgcact tccaggccaa cttcgccagc gcgctgcggc cggcattggc
     3601 aagcgatcct gcagccaccg acgcgcgtgc gctgccgtcg gacgcgcgcg ggctcacgct
     3661 ggcgcaacgc accggcgcac tggtgcagcg cgccgccgca cgcgcgctgc aggttccgtt
     3721 cgtcgcgctg gtcgcggagc cgctgctgca gcacgacttc aacagctgga tcgagatcca
     3781 ggcgacaacc gcgccgctgg atcgcggcag ccgcgacctg ctgccgcaaa gcgaaaagct
     3841 cgcggcactg ggcattgcgc ccacgcagcg cagcggcgac gaaggcccgg ttgccgacag
     3901 ctgggccggc gaagcgccgg gcggattcgc gcaggtgtcg atgctgcaac tggacaagcg
     3961 ccacctgccg ccgcgtctcg ccgcggcgct gggcgatgag ccgtttcacc tggccgcgac
     4021 catcgtcaac acggtcgagc agaaataagg cggagcagaa acaagcggcg cgtgcggccg
     4081 aaaactgctc caccgcaccg ccattttgca tgttattaat tattgattgt ctttataatt
     4141 gaataataat ttgtaacacg tgaaacccgg gggcggtggg tggaaaaaag ggcatcgatg
     4201 accggccggc ggcatccggg cgctcgcgca tgagcacgcc cgcgctgccg ctcacctccg
     4261 cgcagcgcaa gcgcgaggtg ctggcgctgg cgttgcccat catcggcggc atggtgtcgc
     4321 agaacctgct caacctggcc gatgcctgga tggtgggcga tctcggcgcc gccgcgctgg
     4381 ccgccaccgg catggccaac ttcctcaact tcatggcgca ggccttcatc accggtttct
     4441 cgccggcggt gcaggccatc gccgcgcggc gcatgggcga aggccgccgc agcgaggccg
     4501 cggtgccgct caacggcggc ctgctgctgt cgctgatgat cggcatcccg gtcaccttca
     4561 tcggcgtgat cgcggcgccg tggatttttt ccgcgctgaa ccacgacgcc gacgtggtgc
     4621 gccagggcac gccgtacctg caatggcggc tggtgtcggt ggcggcggtg ggcatgaact
     4681 tctccttccg cggctactgg agcgcggcca agctcgccgg cttctacctg caaactcttc
     4741 tgctgatgca cgtgctcaac gtcatcttca gctggaacct gatccacggc atccccgccc
     4801 t
//
LOCUS       DASZZC010000503         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_618701,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000503 DASZZC010000000
VERSION     DASZZC010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618701"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VHE37_10680"
     CDS             complement(<1..1089)
                     /locus_tag="VHE37_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT36044.1"
                     /translation="MDKKFRPQTTLDTEVTYLKGVGPALAQRLRPLNIERVRDLLFHL
                     PSRYEDRRHLVRLDQLADGAEVLVRGKVLHAEVRYAGRRSLRVLIDDGSASALLRFFH
                     FNEQQKTSFAEGRWVQAYGTARGGAKGYELIHPEYRVADQPDAFSVEAGLTPIYPLAT
                     GVTQARLRTLAGTALDLAQRDAAFNTPLHGLDAPDTLAALRALHHPQSGDEAARLLAL
                     KHPAQLRLIREELLAHQLCMRLLRSQVKQRPAPALPDVARAAQELLGALPFRYTRAQQ
                     RVLDDIAADLAQRRPMLRLVQGDVGSGKTVVAAAAMLAAARAGRQSALMAPTELLAEQ
                     HHRNFTRWLQPLGLPVELLAGKLKKGERE"
     gene            complement(1082..1465)
                     /locus_tag="VHE37_10685"
     CDS             complement(1082..1465)
                     /locus_tag="VHE37_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVT36045.1"
                     /translation="MSRKIIQSDQVPAAIGTYSQAVRCGNTVYLSGQIPLDPKTMQMV
                     NGSIDDEIHQVFKNLAALAQASGGSLADAAKVNIFLTDLANFAKVNEIMAQYFKTPYP
                     ARAAVGVASLPRGARVEADAILVLG"
     gene            complement(1630..>2129)
                     /locus_tag="VHE37_10690"
     CDS             complement(1630..>2129)
                     /locus_tag="VHE37_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HVT36046.1"
                     /translation="APLVARHFLPEAAGQQVAPGDALALAVEGTEGLVLTYGKCCHPL
                     PGDDIRGNVSVGRGIVVHRLECKHAKSRPQDWVPLVWSEAVQGDYIAELKLKAGNQRG
                     LLARVTAEISAADSSIENVQMPDRAGSEAIEMRFLITVKNRIHLARVMKRLRRIEHVE
                     RVTRG"
BASE COUNT          319 a          737 c          717 g          356 t
ORIGIN      
        1 ctcgcgctcg cccttcttca gcttgcccgc cagcaattcc accggcaggc ccagtggctg
       61 cagccagcgg gtgaagttgc ggtgatgctg ctcggccagc aattcggtcg gcgccatcaa
      121 cgcggactgc cgcccggcgc gcgcggcggc gagcatggcc gcggcggcaa ccaccgtctt
      181 gcccgagccg acgtcgcctt gcaccaggcg cagcatcggc cggcgctgcg ccagatcggc
      241 ggcgatgtca tccagcacgc gctgctgcgc gcgcgtgtag cggaacggca gcgcgcccag
      301 cagttcctgc gcggcacgcg caacatcggg cagcgccggc gccgggcgct gcttcacctg
      361 gctgcgcagc aggcgcatgc acagctggtg cgccagcaac tcctcgcgga tcagccgcag
      421 ctgcgccgga tgtttcagcg cgagcaggcg cgccgcctca tcgccggact gcgggtgatg
      481 cagcgcgcgc agcgcagcca gcgtatcggg cgcgtcgagc ccgtgcagcg gcgtgttgaa
      541 ggcggcgtcg cgttgcgcca gatccagcgc cgtgcccgcc agcgtgcgca ggcgcgcctg
      601 ggtcacgccg gtggccagcg ggtagatcgg cgtgagcccg gcctcgacgg agaacgcgtc
      661 gggctgatcg gccacgcggt actcgggatg gatcagctcg tagcccttgg cgccgccgcg
      721 cgccgtgcca taggcctgca cccagcggcc ctcggcaaag ctggttttct gctgctcgtt
      781 gaaatggaag aagcgcagca gcgcgctggc gctgccgtcg tcgatcagca cgcgcaggct
      841 gcgccgcccg gcgtagcgca cttccgcgtg cagcactttt ccacgcacca gcacctcggc
      901 gccgtcggcc agctggtcga ggcgcaccag gtggcgccgg tcttcatagc gcgacggcag
      961 atggaacagc aggtcgcgca cgcgctcgat gttcagcggc ctgaggcgct gcgccagtgc
     1021 cgggccgacg cccttgaggt aggtgacctc ggtatcgagc gtcgtctgcg ggcggaattt
     1081 tttatccaag caccaggatc gcgtcggctt ccacgcgcgc accgcgcggc agcgacgcca
     1141 cgcccaccgc ggcgcgcgcc gggtacggcg tcttgaagta ctgcgccatg atctcgttga
     1201 ccttggcgaa gttggccagg tcggtgagga agatgttgac cttggccgcg tcggccagcg
     1261 agccgccgga ggcctgcgcc agcgcggcaa ggttcttgaa cacctggtgg atctcgtcgt
     1321 cgatgctgcc gttcaccatc tgcatggtct tgggatcgag cgggatctgc cccgacaggt
     1381 acaccgtgtt gccgcagcgc acggcctgcg aataggtgcc gatggcggcg ggaacctggt
     1441 cgctctggat gatcttcctg ctcatgtcac tgcactcgct tcatgccggt gggaaaggtg
     1501 cgcgattgtc cgcgcaagcg ctgcgcaatg ccaaccgcac cccggttttg tatgttatta
     1561 attatttatc gaatgtaaaa atattcaata attaataaca ggcggaatgc cggtgcggtg
     1621 gaacggtttt cagcctcggg tgacgcgctc cacgtgctcg atgcggcgca ggcgcttcat
     1681 cacgcgcgcc aggtggatgc ggttcttcac cgtgatcagg aaacgcatct cgatcgcttc
     1741 gctgccggcg cggtcgggca tctgcacgtt ttcgatcgac gaatccgccg ccgagatttc
     1801 cgcggtgacg cgcgccagca gaccgcgctg gttgccggcc ttcagcttca gttcggcgat
     1861 gtaatcgccc tgcaccgcct cgctccacac cagcggcacc cagtcctgcg gccgcgactt
     1921 ggcgtgcttg cactccagcc ggtgcaccac gatgccgcgg cccaccgaca cgttgccgcg
     1981 gatgtcgtcg cccggcagcg ggtgacagca cttgccgtag gtgagcacca ggccctcggt
     2041 gccctccacc gccagcgcca gcgcgtcgcc cggcgccacc tgctggcccg cggcttccgg
     2101 caggaaatgc cgcgccacca gcggcgcca
//
LOCUS       DASZZC010000504         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1007299,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000504 DASZZC010000000
VERSION     DASZZC010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007299"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..999)
                     /locus_tag="VHE37_10695"
     CDS             complement(31..999)
                     /locus_tag="VHE37_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36047.1"
                     /translation="MLRKILLASACLLASCSTQQMLNTVTPENGYDVASNLIYDAEHN
                     LRADIYTPTAAVGAPVVVYFGGLRWSEGDKADYKFVGQALASRGFVCVIPNVREYPQV
                     RFPAFVQDGANAIKWTRQHVGLYGGDLEKLIIMGHSSGAHIAAMLALDEEWLKAVGGS
                     RAWLRGMIGLAGPYDFLPLTDPDLRDIFGPPEDFEKGQPIFYVDGQNPPLLLIHGEDD
                     DSVLVKNSINLADAVRHAGGAVETVIYPKLSHRFALITMSAPLRGQSDVLDNVTDFIK
                     RVAVARALNNEVQGVETQAAPPPAPLPPPTQMLPPPPAADAPTPVQ"
     gene            1089..>1624
                     /locus_tag="VHE37_10700"
     CDS             1089..>1624
                     /locus_tag="VHE37_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVT36048.1"
                     /translation="MLSTSTLITAAVLLYLVVIYNGLVTLRNNLTRNWSNVDVILKQR
                     HDEIPKLVEVCKQHMQFEQETLRGVMLARAGIDSARVQQDVRTLGAAEGALRSGLGSI
                     AATVERYPELRANDSIMQLLGRITALENTISDRREFYNESVNLYNVRTEQFPDVLVAR
                     PFGFRPGVLLEFTAAETA"
BASE COUNT          275 a          531 c          564 g          254 t
ORIGIN      
        1 ttcgaccacc ttgacgaaat cgcccatggc ctactgcacc ggcgtcggcg catcggccgc
       61 gggcggcggc ggcagcatct gcgtgggcgg cggcagcggc gccggcggcg gcgcggcctg
      121 cgtctccacg ccctgcacct cgttgttcaa ggcgcgcgcc acggcgacgc gcttgatgaa
      181 gtcggtgacg ttgtcgagca cgtcggactg cccgcgcagc ggcgcggaca tggtgatcag
      241 cgcgaagcgg tgcgagagtt tcgggtagat caccgtctcc accgcgccgc cggcgtggcg
      301 caccgcatcg gccaggttga tggagttctt caccagcacc gagtcgtcgt cctcgccgtg
      361 gatcagcagc agcggcgggt tctggccgtc gacgtagaag atcggctggc ctttctcgaa
      421 atcttccggc ggcccgaaga tgtcgcgcag gtcagggtcg gtcagcggca ggaagtcgta
      481 cgggccggcc aggccgatca tgccgcgcag ccaggcgcgc gagccgccca ccgccttcag
      541 ccattcctcg tccagggcga gcatggcggc gatgtgcgcg ccggaggagt ggcccatgat
      601 gatgagtttt tccaggtcgc cgccgtaaag gccgacatgc tggcgcgtcc acttgatggc
      661 gttggcgccg tcctgcacga aggcggggaa gcgcacctgc ggatactcgc gcacgttggg
      721 gatcacgcag acgaagccgc gcgaagccag cgcctggccg acgaacttgt agtcggcctt
      781 gtcgccttcg ctccagcgca ggccgccgaa gtacaccacc accggggcgc cgaccgcggc
      841 ggtgggcgtg tagatgtcgg cgcgcaggtt gtgctcggca tcgtagatca ggttgctggc
      901 cacgtcatag ccgttctcgg gcgtgaccgt gttgagcatc tgctgggtgc tgcacgaggc
      961 gagcaggcag gcggaggcga gcagaatctt gcggagcatc gaaaaatttt ccatcgaaaa
     1021 tccacgcggg gcgtaatggt agcgcgcggc ggtgttttgc cgcatcctgc ccgcagggag
     1081 aaatacacat gctcagcacc agcacgctca tcaccgccgc ggtcctgctc tacctggtcg
     1141 tcatctacaa cgggctggtc acgctgcgca acaacctcac gcgcaactgg agcaacgtcg
     1201 acgtcatcct caagcagcgc cacgacgaga ttcccaagct ggtcgaggtg tgcaagcagc
     1261 acatgcagtt cgagcaggag acgctgcgcg gcgtgatgct ggcgcgcgcc ggcatcgaca
     1321 gcgcgcgcgt gcagcaggac gtgcgcacgc tcggcgcggc ggaaggcgcg ctgcgcagcg
     1381 gcctgggcag catcgcggcg acggtggagc gctacccgga actgcgcgcc aacgacagca
     1441 tcatgcagct gctcggccgc atcacagcgc tggagaacac catcagcgac cggcgcgagt
     1501 tctacaacga gagcgtgaat ctctacaacg tgcgcaccga gcagttcccc gacgtgctgg
     1561 tggcgcggcc gttcggcttc cgccccggcg tgctgctgga gttcaccgcg gccgagactg
     1621 ccga
//
LOCUS       DASZZC010000505         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1134060,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000505 DASZZC010000000
VERSION     DASZZC010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134060"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VHE37_10705"
     CDS             complement(<1..545)
                     /locus_tag="VHE37_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36049.1"
                     /translation="MALGLGEIDVKSKLNQKFSAVIPLSSISADDAETAIVGLASNEE
                     FARAGLERGDYLSSLVFSVRTDGQPRIVVSSAQITREPYLAFLIEVRTRNSRVLREYS
                     VLLDPADYSLAASKPAASTPAASAPASSQFYQTPEEAAHTAPAAPAAKPAAPASSSSD
                     YVYVPTPPVSPTVAATRGAAEG"
     gene            complement(726..1748)
                     /locus_tag="VHE37_10710"
     CDS             complement(726..1748)
                     /locus_tag="VHE37_10710"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HVT36050.1"
                     /translation="MSKKYDVAVVGATGAVGEVMLEILHERKFPVGKVYALASERSAG
                     TRVKFGNQELVVEDLASFDFSKVKIGLFSPGASVSAIYAPKAAAAGCVVIDNTSQFRY
                     EKDIPLVVPEVNPQAIAQYKTRGIIANPNCSTIQMLVALKPIYDAVGIERINVCTYQS
                     VSGTGKEAIEELANQTRALLNFQDVPRKVYPKQIAFNVLPHIDIFMDNGYTKEEMKMV
                     WETRKIMGDESILVNPTAVRVPVFYGHSEAVHIETKKKITAEQARELLRSAPGVTVLD
                     ERKDGGYPTAVTEASGKDATFVGRIREDISHPRGLDLWVVSDNIRKGAALNSIQIAEV
                     LIREYL"
     gene            complement(1761..2276)
                     /locus_tag="VHE37_10715"
     CDS             complement(1761..2276)
                     /locus_tag="VHE37_10715"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT36051.1"
                     /translation="MDMKKHVELMCGWHVWAIERFFACVDAASDADYRRDSGLYFKSI
                     HGSVNHMLLVERLWRGRLTGNVIVFDGLDYEIEQDRAKLKSLVLESAKMWRPYVQSLS
                     DAEFHGLLQYKNMAGQAMSLPRIALVHTMFTHGAHHRGQVTTVLTQMGLKTPELDYPY
                     FLAALPRETFN"
     gene            complement(2287..>3186)
                     /gene="leuB"
                     /locus_tag="VHE37_10720"
     CDS             complement(2287..>3186)
                     /gene="leuB"
                     /locus_tag="VHE37_10720"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HVT36052.1"
                     /translation="TQAAARAADAILMGAVGGPKYDELPRELRPERGILGIRKDLNLF
                     ANLRPALAYEELADTSSLKKEVIAGLDILILRELTGDIYFGQPRGRRRNAQGVEEGYD
                     TMHYSVPEVERIARVAFEAARKRNRRVCSVDKENVLETSRLWREVVTRVGLEYPDVQL
                     THMYVDNAAMQLLKKPKQFDVIVTGNIFGDILSDEASMLAGSIGMLPSASLDENNKGL
                     YEPIHGSAPDIMNQDKANPLATILSVAMMFKYSFNRPEVSERIERAVRAVLRAGKRTG
                     DIAMPGEKIIGTREMGDAVVAAL"
BASE COUNT          528 a         1038 c          989 g          631 t
ORIGIN      
        1 ccctccgcgg ctccgcgcgt tgccgccacc gtgggtgaca ccggcggcgt gggcacgtac
       61 acgtagtcgg aggatgacga cgccggtgca gccggcttcg cggcaggtgc agccggcgcg
      121 gtgtgcgccg cttcttccgg cgtctggtag aactgggaag aagccggagc cgaagcggcc
      181 ggcgtgctgg ctgcgggctt gctcgctgcc agcgaatagt ccgccgggtc gagcaacacc
      241 gagtactcac gcagcacgcg cgagttgcgc gtgcgcacct cgatcaggaa cgcgagatac
      301 ggctcgcgcg tgatctgcgc gctgctcacc acgatgcgcg gctggccatc ggtgcgcacg
      361 ctgaacacca ggctcgacag gtaatcgccg cgctcgagac cggcgcgggc gaattcctcg
      421 ttgctggcca ggccgacgat ggcggtttcg gcatcgtcgg cactgatcga actcagcgga
      481 atgaccgcgg agaatttctg gttgagtttg gacttgacgt cgatctcgcc gagtcccagg
      541 gccacggctg aggacagcca caggacggaa acgatcgccg ccaatcgagc gaatatccga
      601 agcatgttca cgtctgcccc cggtctagcc ggaaaaatcc atttaaggtg gtgcgttagt
      661 ttacgcttat aactcctttt atacactata aatagtcacg gcaaaagggg gccgttttcg
      721 ccctttcaga ggtactcgcg gatcagcacc tcggcgatct ggatgctgtt gagcgccgcg
      781 cccttgcgga tgttgtcgga caccacccac agatcgagcc cgcgcgggtg ggaaatgtcc
      841 tcacggatgc ggcccacgaa cgttgcgtcc ttgcccgaag cctcggtcac cgcagtcggg
      901 taaccgccat ccttgcgctc gtcgagcacg gtcacaccgg gcgcgctgcg cagcagttcc
      961 ctcgcctgtt cggccgtgat ctttttcttc gtctcgatgt gcacggcctc ggaatgcccg
     1021 tagaacaccg gcacgcgcac cgcggtgggg ttcaccagga tggattcgtc gcccatgatc
     1081 ttgcgcgtct cccacaccat cttcatttct tccttggtgt agccattgtc catgaagatg
     1141 tcgatgtgcg gcagcacgtt gaacgcgatc tgcttggggt acaccttgcg cggcacgtcc
     1201 tggaagttca gcagcgcgcg cgtctggttg gccagctcct cgatggcttc cttgccggtg
     1261 ccggacacgg attgatacgt gcagacattg atgcgctcga tgccgaccgc gtcgtagatg
     1321 ggcttgagcg ccaccagcat ctggatggtc gagcagttcg ggttggcgat gatgccgcgc
     1381 gtcttgtact gcgcgatggc ctgcggattg acctccggca ccaccagcgg gatgtccttc
     1441 tcgtagcgga actgcgaggt gttgtcgatc accacgcagc cggccgccgc cgccttgggc
     1501 gcatagatgg cggacacgct cgcgcccggg gagaacaggc cgatcttcac cttggagaaa
     1561 tcgaagctgg cgaggtcttc gaccaccagt tcctggttgc cgaacttcac gcgcgtgccg
     1621 gcgctgcgct cgctggccag cgcgtagacc ttgccgaccg ggaacttacg ctcatgcagg
     1681 atctccagca tgacttcacc gactgcgccg gtggccccga ctaccgcgac atcgtacttc
     1741 ttgctcatgt gccttcttct tcagttgaac gtttcgcgcg gcagcgccgc gaggaaatag
     1801 gggtaatcaa gctccggcgt tttcagcccc atctgcgtca gcaccgtggt gacctgtccg
     1861 cggtgatgcg cgccatgcgt gaacatggtg tggaccagcg cgatgcgcgg cagcgacatc
     1921 gcctgcccgg ccatgttctt gtactgcagc aggccgtgaa actcggcgtc ggacaatgac
     1981 tgcacgtacg gccgccacat tttcgccgac tccagcacca gcgacttcag cttcgcgcgg
     2041 tcctgttcga tctcgtagtc gaggccgtcg aacacgatca cattgccggt gagccgtccg
     2101 cgccacagcc gctccaccag cagcatgtgg ttgacgctgc cgtggatgct cttgaagtac
     2161 agcccgctgt cgcgccggta atcggcatcg ctcgccgcgt cgacgcaggc gaagaaccgc
     2221 tcgatggccc agacgtgcca gccgcacatc agctccacgt gcttcttcat gtccatgatc
     2281 cgctcctcac aacgcggcga cgaccgcgtc gcccatctcg cgcgtgccga tgatcttctc
     2341 gcccggcatg gcgatgtcgc cggtgcgctt gcctgcgcgg agcaccgcgc gcaccgcgcg
     2401 ctcgatgcgc tccgacactt ccgggcggtt gaagctgtac ttgaacatca tcgccaccga
     2461 aagaatcgtg gcgagaggat tcgccttgtc ctggttcatg atgtcgggcg cgctgccgtg
     2521 gatcggctcg tacagaccct tgttgttctc atccagcgag gccgacggca gcatgccgat
     2581 ggaacctgcc agcatcgagg cttcgtcgga aagaatgtcg ccgaagatgt tgccggtgac
     2641 gatgacgtcg aactgcttgg gctttttcag cagttgcatg gccgcgttgt ccacgtacat
     2701 gtgcgtgagc tgcacgtcgg ggtattcgag gccgacgcgc gtcaccacct cgcgccacag
     2761 gcgcgatgtc tccagcacgt tttccttgtc cacgctgcag acacgacgat tcctcttgcg
     2821 cgccgcttca aaggcgacgc gcgcaatgcg ctccacctcc ggcacgctgt agtgcatggt
     2881 gtcgtagcct tcctcgacgc cctgcgcatt cctgcgccgg ccgcgtggct ggccgaaata
     2941 aatgtcgccg gtcagctcgc gcagaatcag gatgtccagc cccgcgatca cttctttctt
     3001 cagcgacgac gtgtcggcca gctcttcata ggcgagcgcc ggccgcaggt tggcaaacag
     3061 gttgagatcc ttgcggatgc ccaggatgcc gcgctcgggc cgcagctcgc gcggcagttc
     3121 gtcgtacttt ggcccgccga ctgcacccat caggatcgcg tcggccgcgc gcgcggcggc
     3181 ctgcgt
//
LOCUS       DASZZC010000506         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_679244,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000506 DASZZC010000000
VERSION     DASZZC010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679244"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VHE37_10725"
     CDS             complement(<1..953)
                     /locus_tag="VHE37_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HVT36053.1"
                     /translation="MTQKLIRIGCASAFWGDTNTAAAQLVEKGDIDYLVFDYLAEITM
                     SILAAKRMKDANDGYATDFVEHVMAPLMPAIRAKNIKVVANAGGVNPLACKKALEAAA
                     EKAGVKFRVAVVVGDDLLPRKKDFAACTELDSGKPMPATLVSMNAYLGALPIARALDA
                     GAEIVITGRCVDSAVVLGPLVHEFGWQENDYDRLAAGSIAGHIVECGAQCTGGNYTDW
                     ELVKDGYADMGFPIVECAADGSFEVTKPAGTGGLVSVHTVLEQMLYEIGDPHRYLLPD
                     VTCDFTQVQLAQAGEHRVRVSGCHGYAPTGTYKVSATYPAG"
     gene            complement(950..1489)
                     /locus_tag="VHE37_10730"
     CDS             complement(950..1489)
                     /locus_tag="VHE37_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HVT36054.1"
                     /translation="MSDESEDAGAPAPPAALVLTTPEHFIAFGFGAGLARTAPGTWGT
                     LVGVPFFLALQFLPVPAYLAACVLFFVFGCWVCGESARLLRVHDYGGIVFDEIAGFLI
                     ACVPLVRGAGLVHQWLAAWLLAAFALFRFFDIIKPWPIGWFDRRLRGGFGIMFDDALA
                     GIFTAAWLLLLLELVRWKA"
     gene            complement(1486..2526)
                     /gene="thiL"
                     /locus_tag="VHE37_10735"
     CDS             complement(1486..2526)
                     /gene="thiL"
                     /locus_tag="VHE37_10735"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HVT36055.1"
                     /translation="MDEFGIIKKYFEPLGKPRKDITLGIGDDAALLQMLPGQELVVTT
                     DTLTVDHHFFHDGDPYSIGWKSLAVSLSDLAAMGAMPQWCTMALTLPNADEEWLAEFA
                     RGFKDLADVNHIVLVGGNLSHGPMSITVTAIGSVIAGTAMKRRGARAGDLVCVTGTLG
                     DAALGLRMAMLLRRAEAKAGRKLWRRSSDADDPIEPGFALNTEPSALEALYLRGRLDK
                     PDPRVKAGLILRQAATAGIDLSDGLAGDLQHILTASGVGAEIYADRLPTSEAFAKIAP
                     PAFRFQLQLAGGDDYELCVTLPADVAAEMRRKLDVPLTIVGRITGVTGLRYLNASGAV
                     IPAKLRGFQHFT"
     gene            complement(2542..2988)
                     /gene="nusB"
                     /locus_tag="VHE37_10740"
     CDS             complement(2542..2988)
                     /gene="nusB"
                     /locus_tag="VHE37_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVT36056.1"
                     /translation="MNESAPQSRRGLARRLTVQALYQWLVNETAPAALLVQFREQPEG
                     LGRADGDYFNELLNGAVAQAAALDVLLAPHLDRPLVQLDPVEHAILLLGAYELSHKPE
                     VPWKVAVNEAVNLSKLFGAEEGYKFVNGVLDKYARVARGAEINAGL"
     gene            complement(2985..3476)
                     /gene="ribH"
                     /locus_tag="VHE37_10745"
     CDS             complement(2985..3476)
                     /gene="ribH"
                     /locus_tag="VHE37_10745"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVT36057.1"
                     /translation="MNKSGYAAPAAPAPGEFADARIAIVATRWNVEIVDALIDGARRC
                     LSDWGVPADRVKLFRAPGAYELPLACSALLDSGGYDGVVALGVVIRGDTPHFDFVAGE
                     CARGLMDVQLSHGLAVGFGVLTVNTVQQALERAGAGDDNKGYEAAAAALEMVRLVRGS
                     EAA"
     gene            complement(3487..>4433)
                     /locus_tag="VHE37_10750"
     CDS             complement(3487..>4433)
                     /locus_tag="VHE37_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HVT36058.1"
                     /translation="GLICLTLTPERCRQLRLPQMVARNEESHRTAFTVSIEAAQGVTT
                     GISAHDRARTVQAAVAREAKPDDLVQPGHIFPLMAQPGGVLTRAGHTEAGCDLARLAG
                     LEPASVIVEILNEDGTMARRPDLEKFARQHGLKLGTIADLIRYRLDNEHTIERVAETN
                     VQTEFGEFHLVTYQDSLDSTVHLALVKGTLDPKQPTLVRVHMRNMLSDVIGVEHGNFT
                     WPLRRALKRVADEGAGVVVLLRKPESPREVMQQIVALNKPQAEEAHDPGTVLRTYGIG
                     AQILADLGVRRMRVLSLPKRMQGISGFGLEVVEYVPCD"
BASE COUNT          682 a         1537 c         1518 g          696 t
ORIGIN      
        1 atgccggccg ggtaggtcgc gctcaccttg taggtgccgg tgggcgcgta tccgtggcag
       61 ccgctgacgc gcacacggtg ctcgccggct tgcgccagct gcacctgggt gaaatcgcag
      121 gtcacatcgg gcagcaggta gcggtgcgga tcgccgatct cgtagagcat ctgctccagc
      181 accgtgtgca cggacaccag gccgccggta ccggccggct tggtcacctc gaaagagcca
      241 tcggccgcac attcgacgat ggggaagccc atgtcggcat agccgtcctt caccagctcc
      301 cagtcggtgt agttgccgcc ggtgcattgc gcgccgcact cgacgatgtg gccggcgatg
      361 ctgccggcgg cgagcctgtc gtagtcgttt tcctgccagc cgaactcgtg caccagcgga
      421 ccgagcacca cggccgaatc gacgcagcgc ccggtgatga cgatctccgc gcccgcatcg
      481 agcgcgcgcg cgatgggcag cgcgccgaga taggcgttca tcgacaccag cgtggccggc
      541 atcggcttgc cgctgtcgag ctcggtgcac gccgcgaaat ccttcttgcg cggcagcagg
      601 tcgtcgccca ccaccaccgc cacgcggaac ttcacgccgg ctttttccgc cgcagcttcc
      661 agtgccttct tgcacgccag cgggttgacg ccaccagcgt tggccaccac cttgatgttc
      721 ttcgcgcgga tggccggcat cagcggcgcc atcacgtgct cgacaaagtc ggtggcgtag
      781 ccgtcgttgg cgtccttcat gcgcttggcg gcaaggatcg acatggtgat ctcggccagg
      841 tagtcgaaca ccaggtagtc gatgtcgccc ttctccacca gctgcgcggc ggcggtgttg
      901 gtatcgcccc agaacgcgga ggcgcagccg atgcggatca gtttttgtgt catgccttcc
      961 accggaccag ttccaggagc agcagcagcc acgccgcggt gaagatgccg gccagcgcgt
     1021 catcgaacat gatgccgaag ccgccgcgca gcctgcggtc gaaccagcct atcggccacg
     1081 gcttgatgat gtcgaagaag cggaacagcg cgaatgccgc cagcagccac gccgccagcc
     1141 actggtgcac caggccggcg ccgcgcacca gcggcacgca ggcgatgaga aaaccggcga
     1201 tctcgtcgaa cacgatgccg ccgtagtcgt gcacgcgcag cagccgcgcg ctctcgccgc
     1261 agacccagca gccgaagacg aagaacagca cgcacgccgc caggtacgcc ggcaccggca
     1321 ggaattgcag cgcgagaaag aacggcacgc cgaccagcgt gccccaggtg ccgggcgccg
     1381 tgcgtgccag gccggcgccg aatccgaacg caatgaagtg ctcgggcgtg gtcagcacca
     1441 gcgcggccgg cggcgcaggc gcaccggcat cttcgctttc gtcgctcatg tgaagtgctg
     1501 gaagccgcgc agcttggcgg gaatcaccgc gccgctggca ttgagatagc gcaggcccgt
     1561 cactccggtg atgcggccga cgatggtcag cggcacgtcg agcttgcgcc gcatctccgc
     1621 cgccacatcc gcgggcagcg tcacgcagag ttcgtagtcg tcgccaccgg ccagctgcag
     1681 ctggaagcgg aaggccggcg gcgcgatttt ggcgaaggcc tcggaggtcg gcaggcggtc
     1741 ggcgtagatc tccgcgccca cgccgctggc ggtgaggatg tgctgcaggt cgccggccag
     1801 gccatccgac aggtcgatgc cggcggtcgc tgcctggcgc aggatcagcc cggccttgac
     1861 ccgcggatcg ggcttgtcga gacggccgcg caggtacagc gcttccagcg cgctgggctc
     1921 ggtgttgagc gcgaagccag gctcgatcgg atcgtcggca tcgctgctgc gccgccacag
     1981 cttgcgtccg gccttggcct ccgcgcgacg cagcagcatg gccatgcgca ggccgagcgc
     2041 agcatcgccg agcgtgccgg tgacgcagac caggtcgccg gcgcgggcgc cgcggcgctt
     2101 catcgcggtg ccggcgatca ccgagccgat ggcggtgacg gtgatcgaca tgggcccgtg
     2161 cgagaggttg ccgcccacca gcacgatgtg gttgacgtcg gccaggtcct tgaagccgcg
     2221 cgcaaactcg gccagccact cttcatcagc gttgggcagg gtcagcgcca tggtgcacca
     2281 ctgcggcatg gcgcccatgg cggcaagatc cgacagcgac accgccagcg atttccagcc
     2341 gatggaatac ggatcgccgt cgtggaagaa atgatggtcg acggtgagcg tgtcggtggt
     2401 caccaccagt tcctggccgg gcagcatctg cagcagcgcg gcatcgtcgc cgatgcccag
     2461 cgtgatgtcc ttgcgcggct tgcccagcgg ctcgaagtat ttcttgatga tgccgaattc
     2521 gtccacggcc cttccctggc gttacaaccc ggcgttgatt tcggcgccgc gcgccacgcg
     2581 cgcgtacttg tcgagcacgc cgttgacgaa cttgtagccc tcctccgcac cgaacagctt
     2641 ggacaggttg accgcctcgt tcaccgccac cttccacggc acctcgggct tgtggctcag
     2701 ttcgtaggcg ccgagcagca ggatggcgtg ctccaccggg tcgagctgca ccagcgggcg
     2761 gtccaggtgg ggcgccagca gcacgtcgag cgccgccgcc tgcgccaccg cgccgttgag
     2821 cagctcgttg aaatagtcgc cgtcggcgcg gcccaggcct tccggctgct cgcggaactg
     2881 caccagcagt gcggcaggtg cggtttcgtt gaccagccac tggtatagcg cctgcacggt
     2941 gagccggcgc gccaggccgc ggcggctctg cggggcggat tcgttcatgc tgcctccgac
     3001 ccgcgcacca ggcggaccat ttccagcgcg gcggcagcgg cctcgtaacc cttgttgtcg
     3061 tcgcccgcgc cggcgcgctc caacgcctgc tgcacggtgt tcaccgtgag cacgccgaaa
     3121 cccacggcga gtccatgcga cagctgcacg tccatcagcc cgcgcgcgca ttcgccggcg
     3181 acgaaatcga aatgcggcgt gtcgccgcgg atcaccacgc ccagcgccac cacgccgtcg
     3241 tagccgccgc tgtcgagcag cgccgagcag gccagcggca gttcataggc gccgggcgcg
     3301 cggaacagtt tcacccggtc cgccggcacg ccccagtcgg acaggcagcg gcgcgcaccg
     3361 tcgatgagcg cgtcgacgat ctcgacgttc cagcgcgtgg cgacgatggc gattcgggcg
     3421 tcggcgaatt cgccgggcgc gggtgcggca ggcgccgcgt agccggattt gttcatcagg
     3481 cttggatcag tcacagggca cgtattcgac cacttccagg ccgaagccgg aaatgccctg
     3541 catgcgcttg ggcagcgaca gcacgcgcat ccggcgcacg cccaggtcgg cgaggatctg
     3601 cgcgcctatg ccgtaggtgc gcagcaccgt gcccggatcg tgcgcctcct cggcctgcgg
     3661 cttgttcagc gccacgatct gctgcatcac ctctcgcggc gattcgggct tgcgcagcag
     3721 caccaccacg ccggcgcctt cgtcggccac gcgtttcaac gcgcggcgca gcggccaggt
     3781 gaaattgccg tgctccacgc cgatcacgtc ggacagcatg ttgcgcatgt gcacgcgcac
     3841 cagcgtgggc tgcttcggat cgagcgtgcc cttgaccagc gccaggtgca ccgtgctgtc
     3901 gaggctgtcc tggtaggtga ccaggtggaa ctcgccgaac tcggtctgca cgttggtctc
     3961 ggccacgcgc tcgatggtgt gctcgttgtc gaggcggtag cggatcaggt ctgcaatcgt
     4021 gccgagcttg agcccgtgct ggcgcgcgaa tttttccagg tcggggcggc gcgccatggt
     4081 gccgtcctcg ttgaggatct cgacgatgac cgacgccggc tccaggcccg cgagacgcgc
     4141 caggtcgcag cccgcctcgg tgtggccggc gcgcgtcagc acgccgccgg gctgcgccat
     4201 cagcgggaag atgtggccgg gctgcaccag gtcgtcgggc ttggcctcgc gcgccaccgc
     4261 ggcctgcacc gtgcgcgcgc ggtcgtgcgc ggagatcccg gtggtgacgc cctgcgccgc
     4321 ctcgatcgac acggtgaacg cggtgcggtg gctctcttcg ttgcgcgcca ccatctgcgg
     4381 caggcgcagc tggcggcagc gctcgggcgt gagcgtgagg cagatcagcc cgc
//
LOCUS       DASZZC010000507         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_492710,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000507 DASZZC010000000
VERSION     DASZZC010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492710"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VHE37_10755"
     CDS             complement(<1..311)
                     /locus_tag="VHE37_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVT36059.1"
                     /translation="MDSPLHLIDDDERIAQLLRGARRVAVLGIKTEAQSGAPAYYVPE
                     YLKRAGYELIPVPVYYPEAREILGYPVQRSLQDIPGGIDIVDVFRRAEDIPQHVADLL
                     AL"
     gene            complement(343..771)
                     /gene="queF"
                     /locus_tag="VHE37_10760"
     CDS             complement(343..771)
                     /gene="queF"
                     /locus_tag="VHE37_10760"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HVT36060.1"
                     /translation="MPTTASKTLETFPNPAPRRDYAIRMRMPEFTCLCPKTGQPDFAT
                     LYLEYIADRKCVELKSLKLYLWSFRDEGHFHEDVTNRMLDDLVRALAPRYLRLTAVFN
                     VRGGIYTHVVAEHRAKNFKGALPPAAWHVDPTAQGETPHG"
     gene            962..>2058
                     /locus_tag="VHE37_10765"
     CDS             962..>2058
                     /locus_tag="VHE37_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT36061.1"
                     /translation="MRLSGIKLAGFKSFVDPMHLPLPSNLTGIVGPNGCGKSNIIDAV
                     RWVLGETSMKNLRSSEMEDVIFNGSKSRKPVGRASIELVFDNSDGTVGGPYAAYAEIA
                     VRRELTRDGNSQYYLNGRKCLKRDVTDLFLGTGLGGKNQYAIIEQGMVSRMIEAKPEE
                     LRQWLEEAAGISRYKDRRRETESRIKQTRENLARLNDLRTELQQRLDVLHKQAANAEK
                     YKEYMATERTLRAEILALKVRTLDAQCVAHEAAIADAEKALEEARAQAAAAVTGREEA
                     ENAQREANHALNAEQSKVYEAEAALSRKEQDLGHARQLKEMKLREVEQLGRQLVEVDE
                     RTRREQLRLEEAGRAQIALEDQVQFAQKKEA"
BASE COUNT          414 a          646 c          682 g          316 t
ORIGIN      
        1 agcgccagca ggtccgccac gtgctgcgga atatcctcgg cgcggcggaa cacgtcgacg
       61 atgtcgatcc cgccgggaat gtcctgcagc gagcgttgca ccgggtagcc caggatctcg
      121 cgcgcctccg ggtaatacac cggcactggg atcagctcgt agcccgcacg cttcagatat
      181 tccggcacgt agtacgcggg cgcgccggac tgcgcctcgg tcttgatgcc cagcacggcg
      241 acgcgccgcg cgccgcgcag caactgcgcg atgcgttcgt cgtcgtcgat cagatgaaga
      301 ggggagtcca tgacccttgg aaccgcgtgc ggtgcacgtg gctcagccgt gcggcgtttc
      361 gccctgcgcc gtcgggtcca catgccacgc tgccggcggc agcgcgccct tgaagttctt
      421 cgcgcggtgc tcggccacca cgtgcgtgta gatgccgccg cgcacgttga acaccgcggt
      481 cagccgcagg tagcgcggcg ccagcgcgcg caccaggtcg tcgagcatgc gattggtcac
      541 gtcctcgtgg aaatggcctt cgtcgcggaa gctccagagg tagagcttga gcgacttgag
      601 ctccacgcac ttcctgtccg cgatgtactc gaggtacagc gtggcgaagt caggctggcc
      661 ggtcttcggg cacaggcagg tgaattccgg catgcgcata cggatcgcgt agtcgcgccg
      721 cggcgccggg ttggggaagg tttcgagcgt cttggaggct gtggtcggca taaaattcat
      781 tcgaccgcac tgccgttgtg gctgttatta attattatcc gatattaaaa tcgaataata
      841 ttttgtaaca cgctttgcag gggtgcggtg gggcaaaaaa caggctcgat actttacact
      901 agcgcccgat tttcccgacc acataacaac gcccgcatca ccaagggcga caccgaattc
      961 aatgcgcctt tctggcatca agctcgccgg gttcaagtct tttgtcgacc cgatgcacct
     1021 gccgctgccg tcgaacctga ccggcatcgt cggtcccaac ggttgcggca agtccaacat
     1081 catcgatgcg gtgcgctggg tgctgggcga aaccagcatg aagaacctgc gctcttccga
     1141 aatggaagac gtgatcttca acggcagcaa gagcaggaaa cccgtcggcc gcgcctcgat
     1201 cgagctggtg ttcgacaaca gcgacggcac cgtcggcggg ccgtacgcgg cctacgcgga
     1261 gattgcggtg cgccgcgagc tgacgcgcga cggcaattcg cagtactacc ttaacggccg
     1321 caagtgcctg aagcgcgacg tcaccgacct gttcctcggc accggcctgg gcggcaagaa
     1381 ccagtacgcc atcatcgagc agggcatggt ctcgcgcatg atcgaggcca agcccgaaga
     1441 gctgcgccag tggctggaag aagccgccgg catcagccgt tacaaggacc gccggcgcga
     1501 gacggaatcg cgcatcaagc agacgcgtga aaacctggcg cgcctgaacg acctgcgcac
     1561 cgagctgcag cagcgcctgg acgtgctgca caagcaggcg gccaatgccg agaagtacaa
     1621 ggaatacatg gccaccgagc gcacgctgcg cgcggagatc ctggcgctga aggtgcgcac
     1681 gctcgacgcg caatgcgtgg cgcacgaagc cgccattgcc gatgccgaga aggcgctgga
     1741 agaagcacgg gcgcaggccg ccgccgccgt caccggccgc gaggaagcgg agaacgcgca
     1801 gcgcgaggcc aaccacgcgc tcaatgccga gcagagcaag gtctacgagg cggaagccgc
     1861 gctgtcgcgc aaggaacagg acctgggcca cgcgcgccag ctcaaggaaa tgaagctgcg
     1921 cgaagtggag cagctcggcc gccagctggt ggaagtggac gagcgcaccc ggcgcgagca
     1981 gctgcggctg gaagaagccg gccgcgcgca gatcgcgctg gaagaccagg tgcagttcgc
     2041 gcagaagaag gaagccga
//
LOCUS       DASZZC010000508         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1194871,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000508 DASZZC010000000
VERSION     DASZZC010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194871"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VHE37_10770"
     CDS             complement(<1..1506)
                     /locus_tag="VHE37_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HVT36062.1"
                     /translation="MPSVVSYSVAADPHAHEFLVSCMVAKPASRGQVFALPSWIRGSY
                     LVRDFAKHVSALSARSGARELAIERIDKRSFRVAPVRGPLTLSWRVHAYDESVRKAYL
                     DTRRGFFNGSSLFYLPLGHEHAVFEVQIEKPAAAECADWRIATTLPVEKVGRDGFGRY
                     RARGYEQLIDCPVEMAAYERIDFKVDGIPHALVLSGRYEIDRERVAQDLARICHVERE
                     LFRQQPELDQYLFLTNVTGAGYGGLEHQTCTALICSRNDLPRPGMGKLTRDYRGFLGL
                     CSHEYFHLWNVKRITPESFLDSDLAREAYTRDLWHYEGVTSYYDDLFLLRAGVLDAPG
                     YLDVLAETATRLSRTPGRGAHTLADASFEAWIKYYQPDEQSPNVTVNYYVKGALAALC
                     LDLTLRLRSKVTLDDVMRELWTRYGKRSKGVPEGGLEKIAGELSRQDLKPFFDRALRS
                     TQELPLAELLAEFGVECTQRAQLSEADNGGRAGGEPPGAWLGVKLKPGEARI"
     gene            complement(1715..>2240)
                     /locus_tag="VHE37_10775"
     CDS             complement(1715..>2240)
                     /locus_tag="VHE37_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36063.1"
                     /translation="NGLTFDSTGAEANGGKQRFQGAEIEARYALAGALALAGNASYHD
                     ARFRRFTQDDGSMLDGNRLETAPYTLAGLGLVYAPPRGFNASVTGNYTGPRQLNNPNT
                     LPAGGYTTLDAGLGWRFGQIALSVNGYNLTDRRDPVSASELSEVATNHDGVNTIGYYR
                     LPARSVMAGISLDL"
BASE COUNT          359 a          735 c          771 g          375 t
ORIGIN      
        1 aatgcgcgcc tcgccgggct tgagcttcac gccgagccac gcgcccggcg gctcgccgcc
       61 ggcgcggccg ccgttgtcgg cctcggacaa ctgcgcgcgc tgcgtgcact cgacgccgaa
      121 ctcggccagc agttcggcca gcggcagttc ctgcgtcgaa cgcagcgcgc ggtcgaagaa
      181 cggtttcaga tcctgccgcg acagctcgcc ggcgatcttc tccagcccgc cctcgggcac
      241 gcccttgcta cgttttccgt agcgtgtcca cagctcgcgc atgacgtcat cgagcgtgac
      301 cttcgagcgc aggcgcagcg tgaggtccag gcacagcgcg gccagcgcgc ccttcacgta
      361 gtagttcacc gtgacattcg gcgactgctc atcgggctgg tagtacttga tccacgcctc
      421 gaagctggca tcggccagcg tgtgcgcgcc gcgcccgggc gtgcgcgaaa ggcgcgtggc
      481 ggtttcggcc agcacgtcga gatagccggg cgcgtcgagc acgccggcgc gcagcaggaa
      541 caggtcgtcg tagtagctgg tcacaccctc gtaatgccac aggtcgcgtg tgtaggcctc
      601 gcgcgcgagg tcgctgtcca ggaaactttc cggcgtgatg cgcttgacgt tccacaggtg
      661 gaagtattcg tgcgagcaca ggccgagaaa gccgcggtag tcgcgcgtca gcttgcccat
      721 gccggggcgc ggcaggtcgt tgcgtgagca gatcagcgcg gtgcaggtct ggtgctccag
      781 gccgccgtag ccggcgccgg tgacgttggt caggaacagg tactggtcga gttccggctg
      841 ctggcggaac agctcgcgct cgacgtggca gatgcgcgcc aggtcctgcg ccacgcgctc
      901 gcggtcgatc tcgtagcgcc ccgacagcac cagcgcgtgc ggaatgccat cgaccttgaa
      961 gtcgatgcgc tcgtacgccg ccatctccac cgggcagtcg atcagctgct cgtagccgcg
     1021 cgcgcggtag cggccgaagc cgtcgcggcc gactttttcc accggcagcg tggtggcgat
     1081 gcgccagtcc gcgcattccg ccgcggcggg cttttcgatc tgcacctcga acaccgcgtg
     1141 ctcgtgcccg agcggcaggt agaagagaga tgagccgttg aagaagccgc ggcgcgtatc
     1201 gagataagcc ttgcgcaccg actcgtcgta cgcgtgcacg cgccagctga gcgtgagcgg
     1261 cccgcgcacg ggcgccacgc ggaagctgcg cttgtcgatg cgctcgatgg ccagctcgcg
     1321 cgcgccgctg cgcgcggaca gcgcgctcac gtgcttggcg aaatcgcgca ccaggtagct
     1381 gccgcggatc cacgacggca gggcgaacac ctgcccgcgc gacgccggct tcgccaccat
     1441 gcaggacacg aggaattcat gcgcgtgcgg atcggccgca acgctgtacg agacgacgga
     1501 aggcatgcgc gcattatgcc tgccggccat tttttgctct taccgcaccg gcattttctg
     1561 tgttattaat tgttttaaaa ttttaaatta atacaataat tattaacatc gacgggcggg
     1621 gtgcggtgaa gcaaaaaaat cgccgcgcag catcgcgcag aaaaaaatag gccggcacga
     1681 agccggcctc gaataggcgc tcgcgcgcca cccttcatag atcgagcgag atgcccgcca
     1741 tcacgctgcg cgcgggcagg cggtagtagc caatggtgtt gacgccgtcg tggttggtcg
     1801 ccacttccga cagctcgctg gcggacacgg ggtcacgccg gtcggtgagg ttgtagccgt
     1861 tgaccgacag cgcgatctgc ccgaagcgcc agcccaggcc ggcatcgagc gtggtgtagc
     1921 cgccggccgg cagcgtgttg ggattgttca gctggcgcgg accggtgtag ttgccggtga
     1981 ccgaggcgtt gaagccgcgc ggcggcgcgt agaccaggcc gagcccggcc agcgtgtatg
     2041 gcgcggtctc caggcggttg ccgtcgagca tgctgccgtc gtcctgggta aagcggcgga
     2101 agcgcgcgtc gtggtacgag gcgttgcccg ccagcgccag cgcgccggcc agcgcgtagc
     2161 gcgcctcgat ctccgcgccc tggaagcgct gcttgccgcc gttggcctcg gcacccgtgc
     2221 tgtcgaaggt caggccgttg
//
LOCUS       DASZZC010000509         5597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_492974,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000509 DASZZC010000000
VERSION     DASZZC010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492974"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..724)
                     /locus_tag="VHE37_10780"
     CDS             complement(98..724)
                     /locus_tag="VHE37_10780"
                     /inference="COORDINATES: protein motif:HMM:NF027516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating E1 FCCH domain-containing
                     protein"
                     /protein_id="HVT36064.1"
                     /translation="MKINRLLTLALNAGAAIAAYRICKFGADDTTVIQSAASTDAHIG
                     VSTDVPAGGAGDPIDLVRIGLSPVEYGGSVTRGDLLTSDANGKAVTVTPKSAVLSAAI
                     TAITKANPGAVTAAAHGLSTGDRVAITDVGGMVEVNNKVFKVTVVDVDHFTIGVDSTG
                     FTTYTAGGTVIRVAGASTPIRSIGVAEESGVLGDIGSMTVFPSLVHGS"
     gene            complement(755..1828)
                     /locus_tag="VHE37_10785"
     CDS             complement(755..1828)
                     /locus_tag="VHE37_10785"
                     /inference="COORDINATES: protein motif:HMM:NF021627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HVT36065.1"
                     /translation="MGTTGKSGVRHARALNAEAERVVVLRAFCFELPAGTADKPAPEW
                     IPYLPAPDATGRLVGADGRAWSVKDLRALCAGVKRKLPLDINHSTDLLGKAGHEAPAV
                     GWLTGFEVRADGSTWAKVDWNQRGLNALQGKDYGFISPFFDYDSNGVITRLAGASLVN
                     EPNFTSLALNARQSNEDDTMKEILLALGLAETASATDAVGAIAKLKDDGQRALNSASQ
                     PDIKRFVPRADYDAMETRATNAEKKLQESDKAAHQKAVDEEIAGALKEGKITPATEKY
                     YRGTCATAEGLASFRDFRKSAVAVVDDKLTGDRRPRHEQAGDAKSISDRALNFQKTQR
                     ELGREVSIADAVTHVVEHSAQRQ"
     gene            1955..2389
                     /locus_tag="VHE37_10790"
     CDS             1955..2389
                     /locus_tag="VHE37_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36066.1"
                     /translation="MNEIHLARRVLSEANSEYRELLTQFDIFRAAAAKGLESLRFGTS
                     DLSIDQLSPSAFEIAFIGRKFRISLRTRLENNVLKGIVECAEVRDELGDPATSFELRS
                     DGGTNVKVKIPGWEGRCYFVNDYETASHLLISLLVDALKNTG"
     gene            2406..2870
                     /gene="gvpU"
                     /locus_tag="VHE37_10795"
     CDS             2406..2870
                     /gene="gvpU"
                     /locus_tag="VHE37_10795"
                     /inference="COORDINATES: protein motif:HMM:NF041667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle accessory protein GvpU"
                     /protein_id="HVT36067.1"
                     /translation="MNKPSEDAPGAPRFEHASPDFYLRELVTIVNKTGLTFPITLWVG
                     GSVVSGYLIGGADYFEELGSSLETFFASAKVDDPMSIANSLVEPYRKHYEDIPDEDPP
                     PTYIHLRNARVFAPGQNPMPSVGTIWRGRLSRVDGFSFGNLVPDAERRREPE"
     gene            complement(2955..3431)
                     /locus_tag="VHE37_10800"
     CDS             complement(2955..3431)
                     /locus_tag="VHE37_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36068.1"
                     /translation="MSPGFNVDVAQLPTTMRRLIRAVGVPATMSLLEARGGTRVYLGG
                     QRGRRDRQLLMQLLGEKGEAAFRREFTPPAMSHARSASEVTLPKLDKIATQIRDAAIR
                     ADETHSLTQLALMFKLTSRQIQNIRKGLPTTRIPPGQGDLFAADGRQGSGTRLNGV"
     gene            complement(3428..4078)
                     /locus_tag="VHE37_10805"
     CDS             complement(3428..4078)
                     /locus_tag="VHE37_10805"
                     /inference="COORDINATES: protein motif:HMM:NF018011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HVT36069.1"
                     /translation="MKKPLRNRELGRIHILKEALGLDRATYEAMLWTIGRKESAAELD
                     DYGRRQVIAHQEALLRQVDPKHRALSYPARPRNLDVGDRKELRKIEALLADAGVDWSY
                     AHGLARRMYRRDRVQLCHAGQLAGIIAALEKQALKRLAEQLQQIFGEAWAGQVERIAA
                     QLFGFDWQHRDITRYTEPLSRVLRWWHGELQPACEWPPAEGAYCQGCYERRQARAA"
     gene            complement(4251..4589)
                     /locus_tag="VHE37_10810"
     CDS             complement(4251..4589)
                     /locus_tag="VHE37_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36070.1"
                     /translation="MTPLTRTTVLFALAMHRGEENGVRACDLVAEICEETSPALERQL
                     RHLIEELRLEGEHICGHPSTGYYIAANDEELLKTVRFLHDRAMSSLRQAARMQNVSLP
                     DLKGQLRLPS"
     gene            complement(4592..4780)
                     /locus_tag="VHE37_10815"
     CDS             complement(4592..4780)
                     /locus_tag="VHE37_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36071.1"
                     /translation="MNVKKTVVLTLADEEIVALEYALTTASRIANDRAKNEAWLDGYS
                     PFELVHLVAVWQSLLYSL"
     gene            complement(4777..5040)
                     /locus_tag="VHE37_10820"
     CDS             complement(4777..5040)
                     /locus_tag="VHE37_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36072.1"
                     /translation="MLAYTDLGGVIEFGLYLPGPALLIARGPSSHLRNVVNDLAVRAI
                     DGETLFVPGLDGKPMKIADEYETRMKRINDFRAKVRQRLESRG"
     gene            complement(5043..>5597)
                     /locus_tag="VHE37_10825"
     CDS             complement(5043..>5597)
                     /locus_tag="VHE37_10825"
                     /inference="COORDINATES: protein motif:HMM:NF022807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HVT36073.1"
                     /translation="SMVKPIDKTRDQLVRELISQAQRVSSAIAAFKKSTFDEVAAFVE
                     LSAGEYGIKLGGAKGNVTLVSFDGRYKIVRQVQDSLVFDERLQAAKALIDQCIQEWSQ
                     GSRDEIRVLVNDAFQVNKEGQINTGRVLGLKRLAISDAKWQQAMQAIADSVRVACSRS
                     YVRFYERVGETDQYKPISLDLASA"
BASE COUNT          928 a         1733 c         1834 g         1102 t
ORIGIN      
        1 tgacaatggc caggagcacc ggatccgtcg gaaacggtgc gatggtggga gtgcgggcaa
       61 tgacggtcat agaagtgctt cctcgctgaa agttggttta ggagccgtgc accagcgacg
      121 ggaacacggt cattgagccg atgtcgccga gcacgccgga ctcctccgcg acgccgatgc
      181 tgcggatcgg cgtggaggca ccggccacgc ggatgaccgt gccgccggcc gtgtaggtcg
      241 tgaagccagt ggagtcgacg ccgatggtga agtggtcgac gtcgacgacg gtgaccttga
      301 acaccttgtt gttcacttcc accatgccgc caacatcagt gatggccacg cggtcgcccg
      361 tggagagtcc gtgcgcagct gcggtcacgg caccgggatt ggccttggtg atggcggtga
      421 tcgcagcaga cagcactgcg ctctttggcg tgaccgtgac ggccttgcca ttggcatcgg
      481 acgtgagcag atcgccgcgc gttacgctgc cgccgtattc gacgggggat aggccgatgc
      541 ggacgaggtc gatcggatcg ccggcgccac ctgcgggcac gtcggtcgag acgccgatat
      601 gcgcatcggt actggcagcg gactgaatga cggtggtgtc gtccgcaccg aacttgcaga
      661 tgcggtaagc ggcaatggct gcgcctgcat tgagtgcaag ggtcagcaag cggttgatct
      721 tcatggtgac tcctggtgag tggatttggg ccgcttactg gcgctgggcc gaatgctcga
      781 cgacgtgagt tactgcgtcg gcaatcgaca cctcgcggcc gagctcgcgc tgcgtcttct
      841 ggaagttgag cgcacggtcc gaaatgctct ttgcgtcgcc agcctgttca tgccgcgggc
      901 ggcgatcgcc ggtgagcttg tcgtcgacga cggccaccgc gctcttgcgg aagtcgcgga
      961 agctggcgag gccttcggcg gtcgcgcagg tgccgcggta gtacttttcc gtggcggggg
     1021 tgatcttccc ttccttgagg gcgccggcga tctcttcatc gaccgctttt tggtgcgcgg
     1081 ccttgtccga ctcctgcagc tttttctcgg cgttggtggc ccgcgtttcc atcgcgtcat
     1141 agtcggcacg cggcacaaag cgcttgatgt ccggctggct cgccgaattg agagcgcgct
     1201 ggccgtcgtc tttcagcttg gcgatagcgc caacggcgtc ggtggcggat gcggtctcag
     1261 ccaggcccag cgccagcaga atttccttca tggtgtcgtc ctcgttggat tggcgcgcat
     1321 tcagcgccag ggaagtgaag ttgggttcgt tgaccaggct ggcgccggcg aggcgcgtga
     1381 tgacgccgtt ggagtcatag tcgaaaaagg ggctgatgaa gccgtagtct tttccctgca
     1441 gcgcattcag gccgcgctgg ttccagtcga ccttcgccca ggtgctgcca tcggctcgca
     1501 cctcaaagcc ggtcagccag ccgaccgcgg gagcctcatg cccagccttg ccgagcaggt
     1561 cggtgctgtg attgatgtcg aggggcagct tgcgcttgac gccggcacac aacgctcgaa
     1621 ggtctttgac cgaccaggca cgaccgtcag cgccgaccag ccggccggtg gcgtcgggtg
     1681 ccgggagata gggaatccac tcgggggcgg gcttgtcggc cgtgcccgcg ggaagctcga
     1741 agcagaacgc gcgcaacaca acgacgcgtt ctgcctcggc gttcagcgcg cgggcatgac
     1801 gaactcctga tttccctgtg gtccccattg cgccgccagt gtcggcggcg tagcgtcagt
     1861 aaacagccgg ggaaatgttt cgccccgcgc tgggcggggc gaggttcaac gatagcagcc
     1921 agcgctccaa ccgccaacat cgaagggaaa ttcaatgaat gagatacatc tcgcccgccg
     1981 tgtcctttca gaggcaaact cggaatatcg cgagttgctt acccagttcg acatttttag
     2041 ggccgcagcc gccaagggat tggagagctt gcgttttggt actagcgacc taagcatcga
     2101 ccagctctcg ccctcggcat ttgagattgc cttcatcggc cggaaatttc gtatctcgct
     2161 tcgcacacgt ctcgaaaaca acgttcttaa agggatcgtg gaatgcgccg aagttcgtga
     2221 cgagcttggc gatcctgcaa cctcatttga gctccgaagt gacgggggga caaatgtgaa
     2281 ggtcaagatt ccgggatggg agggccgctg ttacttcgtc aatgactatg aaacggcatc
     2341 ccatctgctt atttcgcttt tggtcgatgc gttgaaaaac acaggctaac tggcaggaga
     2401 gaacgatgaa caagccttcc gaggatgccc caggcgcgcc gcgctttgag cacgcgtcac
     2461 ccgattttta tctgcgggag ctggtgacga tagtcaacaa gaccgggctt acatttccga
     2521 tcacgctctg ggtcggcggc tcggtggttt ctggttacct gatcggcggc gcggattatt
     2581 ttgaggaact cgggtcatcc ttggagactt tttttgcgtc cgctaaggtc gatgatccaa
     2641 tgtccattgc caattcgctt gtcgagccat atcggaagca ctacgaggac atccccgacg
     2701 aggatccgcc gccgacttat attcatttgc gaaatgcgcg ggtttttgcc cccggtcaaa
     2761 atccgatgcc atctgttgga acaatctggc gcggacgcct cagccgggtg gatggcttct
     2821 cattcggcaa cctagtgccg gatgccgaga gaaggcggga gccagaataa cccccgaatt
     2881 tgcgcttcct gggcagggct gcatttttgg ccccatcgcc ccgtgaattt gcccaactcg
     2941 ccgcctagcg cgatttaaac gccatttaaa cgggttcccg agccctgccg gccatccgcc
     3001 gcgaacaaat cgccctggcc gggcggtatc cgggtggtcg gcagcccctt tcggatgttc
     3061 tggatctgcc ggctggtcag tttgaacatc agggccagct gggtgaggct gtgcgtctca
     3121 tcggcgcgga tcgcggcgtc gcggatctgc gtggcgatct tgtccagctt gggcagagtc
     3181 acttccgagg ccgagcgcgc gtgcgacatg gccggcggcg tgaactcccg gcggaaggcc
     3241 gcctcgccct tttcgccaag cagctgcatc agcagctgac ggtcgcgccg gccgcgctgg
     3301 ccgcccaggt agacccgggt gcccccgcgc gcctccagta gcgacatggt ggccggaact
     3361 cccacggcgc ggatcagccg ccgcatggtg gtgggcagct gggcgacgtc gacgttgaac
     3421 ccggggctca tgccgcccgc gcctggcgcc gctcgtagca gccctggcag taggcgcctt
     3481 cggcgggagg ccactcgcat gcgggctgca gctcgccgtg ccaccagcgc agcacgcggc
     3541 tgagcggctc tgtgtatcgg gtgatgtcgc ggtgttgcca gtcaaacccg aaaagctgtg
     3601 ccgcgatgcg ctcgacctgg ccggcccaag cctcaccgaa gatctgctgc agctgctcgg
     3661 ccaggcgctt gagggcctgc ttttccagcg ccgcgatgat gccggccagc tggccggcat
     3721 ggcacagctg tacgcgatcg cgccggtaca tgcggcgcgc caggccatgc gcgtagctcc
     3781 agtcgacgcc ggcgtcggcc agcagcgcct cgatcttgcg cagctccttg cgatcgccga
     3841 cgtccaggtt gcgcggccgc gccgggtagc tgagcgccct gtgcttgggg tcgacctggc
     3901 gcagcagcgc ttcctggtgc gcgatgacct ggcgccggcc gtagtcgtcc agctcggcgg
     3961 ccgattcctt gcggccgatg gtccacagca tggcctcgta ggtggcgcga tccaggccga
     4021 gtgcttcctt gaggatgtgg atgcgcccca gctcacggtt tcgcagcggc ttcttcatgg
     4081 ctgcggccgc ttggtcagat gccaccccgc gcaatgcgcg cagtagtagc tgcgcaggcg
     4141 gtagcggcgc ttcgccgagc agcgcgacgc gacgcgcacg gcaacggatt gcgtagggta
     4201 acgcctcttt gacaggcagc tgcgcgccca ctccctcagc tcctgggcgt tcacgatggc
     4261 agcctcagct ggcccttgag gtcgggcagc gacacgttct gcatgcgtgc cgcctggcgc
     4321 agagacgaca tggcgcggtc gtgaaggaag cggacggtct tgagcagctc ctcgtcgttg
     4381 gcggcgatgt aatagcccgt cgacgggtga ccgcagatgt gctcgccttc caggcgcagc
     4441 tcctcgatga ggtggcgcag ctggcgctcc agcgccggcg atgtctcctc gcagatctcc
     4501 gccaccaggt cgcacgcgcg cacgccgttc tcctcgcccc ggtgcatggc cagggcgaac
     4561 agaacggtgg tgcgcgtgag cggggtcatt ttcagagcga gtagagaagg ctctgccaga
     4621 ccgcgactag gtggacgagc tcaaaaggcg aatagccgtc aagccatgcc tcgttcttcg
     4681 ccctgtcgtt tgcaatccgg ctcgctgttg tgagcgcgta ttcgagagca acgatctctt
     4741 catcggctag agtcagcact actgttttct tcacgttcat ccgcgcgact ccaggcgctg
     4801 ccgcaccttg gcgcgaaaat cattgatgcg cttcatgcgc gtttcgtatt cgtcggcgat
     4861 cttcatcggc ttgccgtcga ggccaggaac gaacaatgtt tcaccgtcaa tcgcacgcac
     4921 ggcgagatcg ttgaccacgt tgcgcagatg cgagctggga ccgcgcgcga tcagcagtgc
     4981 cggcccaggc agatacaagc cgaactcgat gacgccgccc aaatcggtat aggcgagcat
     5041 ggctaggccg acgccaggtc gaggctgatg ggcttgtact gatccgtctc gcccacgcgc
     5101 tcgtagaagc gcacgtagct gcgcgagcag gccacgcgca cgctgtccgc gatcgcctgc
     5161 atggcctgct gccatttggc atcgctgatg gccaggcgct tcagcccgag cacgcggccg
     5221 gtgttgatct ggccttcctt gttcacctgg aaggcgtcgt tgaccagcac gcggatctcg
     5281 tcgcggctgc cctgcgacca ttcctggatg cactggtcga tcagcgcctt cgccgcctgc
     5341 aggcgctcat cgaacaccag gctgtcctgc acctggcgca cgatcttgta gcggccgtcg
     5401 aagctgacca gcgtgacgtt gcccttggcg ccgccaagct tgatgccgta ttcgccggcg
     5461 ctgagctcga cgaacgccgc cacctcgtcg aaggtgctct tcttgaacgc ggcgatcgcc
     5521 gagctgacgc gctgggcctg cgagatgagc tcgcgcacca gctggtcgcg ggtcttgtcg
     5581 atcggcttga ccatgct
//
LOCUS       DASZZC010000510         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_946970,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000510 DASZZC010000000
VERSION     DASZZC010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946970"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VHE37_10830"
     CDS             <1..469
                     /locus_tag="VHE37_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT36074.1"
                     /translation="TRTMEGPGAVSGYTISSDASRLDLHAIHAYLTRESYWARGIPFE
                     TVQRAIGNSLCFGVYEQGSGRQVGFARLITDRATFAYLADVYIDTAHRGRGLSKQLVA
                     AVMAHAEVQGLRRWLLATADAHGLYRQFGWSGLSKPERIMERVFPDIYKNPGS"
     gene            472..1443
                     /gene="rfaD"
                     /locus_tag="VHE37_10835"
     CDS             472..1443
                     /gene="rfaD"
                     /locus_tag="VHE37_10835"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012368661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HVT36075.1"
                     /translation="MIIVTGAAGFIGSNLVMALNARGERDIIAVDDLTDGTKFSNLAQ
                     AEIADYLDKDELLSRVESGRLPKVSAVFHQGACSTTTEWNGKFMMDVNYRYSQALLAY
                     CAAKKIPYIYASSASVYGMGHDGFREDRACENPLNMYAYSKFLFDQYVRRNVLAKKRA
                     KTVRGDATQVVGLRYFNVYGPREAHKGGMASTAFHFNNQLKADGECRLFTGSDGYADG
                     EQRRDFVHVDDVVSMNLWFHDHPAKSGIYNAGTGRAQPFNDVARAVIAWHGKGRIKYV
                     PFPEKLVGRYQSYTQADLTALRQAGYERKFMTVEQGVKKYLDWLNAA"
     gene            1433..1852
                     /locus_tag="VHE37_10840"
     CDS             1433..1852
                     /locus_tag="VHE37_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36076.1"
                     /translation="MRRNAAAAALLLAGCAAHPIRDFTTDGCTLFPDGNWCDCCRVHD
                     EAYWRGGTQEQREAADVKLRDCVLAKSGDKALAETMYYGVRGGGAPVFPTWYRWGYGW
                     EYGRGYAPLSADEQKAADAKLAAYHATHGPLQCGAAR"
     gene            1849..2859
                     /gene="waaF"
                     /locus_tag="VHE37_10845"
     CDS             1849..2859
                     /gene="waaF"
                     /locus_tag="VHE37_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HVT36077.1"
                     /translation="MNDRVLVVGPSWVGDMVMAQSLFMRLQQLRPDCEIDVLAPGWSL
                     PLIARMREVRAGIEMPLGHGAFGFSVRRTLGHSLREKNYTQAIVLPGSWKSALVPFFA
                     RIPRRTGYRGEMRYGLLNDIRALDKNLLKTTVQRFVALAEEGSVSVVPAVPEPRFAVK
                     GSIAVAAASVQGHGTALVGLLPGAEYGPAKQWPAAHYGELAKLLARQNVTCLVFGSKK
                     EQALGEEIRVASGNTAVNLCGQTTLTELVDALAQCKAVVTNDSGPMHVAAAAGVPLVA
                     IYGSSTPDFTPPLTARSRIHYLRLACSPCFERTCPLGHYNCLRQIGAAAVRQSLHELA
                     GV"
     gene            3005..>3487
                     /locus_tag="VHE37_10850"
     CDS             3005..>3487
                     /locus_tag="VHE37_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HVT36078.1"
                     /translation="MSFADRDGYIWYDGALKPWRECTTHVLTYSLHYGVGCFEGVRAY
                     QTPKGTAIFRLQDHTRRLLNSAKILGMKVPYSAEVLNQAQLEVLRANKLDSAYLRPMM
                     FYGAEGMGLRADNLKTHVIVAAWTWGSYLGAENMERGIRVKTSSFTRHHVNAAMCRAK
                     A"
BASE COUNT          606 a         1146 c         1154 g          581 t
ORIGIN      
        1 tacgcggact atggaaggac ctggtgcagt gagcggctac accatcagca gcgacgcgtc
       61 gcggctggac ctgcacgcca tccacgccta tctcacgcgc gagtcttact gggcccgcgg
      121 cataccgttc gagacggtgc agcgcgccat cggcaactcg ctgtgcttcg gcgtgtacga
      181 gcagggcagc ggacggcagg tgggatttgc gcggctgatc accgatcgcg cgaccttcgc
      241 ctatctggcc gatgtctaca tcgacacggc gcatcgcggc cgtggcctgt ccaagcaact
      301 ggtggccgcg gtcatggcgc acgcggaggt gcagggactg cgccgctggc tgctggccac
      361 cgccgatgcg cacggcttgt atcgccagtt cggctggagc ggcctatcca agccggagcg
      421 catcatggag cgcgtgtttc ccgacatcta caagaatcca gggagctgaa attgatcatc
      481 gtcaccggcg cagcaggctt catcggctcc aacctcgtca tggcgctcaa tgcgcgcggc
      541 gagcgcgaca tcatcgcggt cgacgatctc actgatggca ccaagttctc caacctggcg
      601 caggccgaga tcgccgatta cctggacaag gacgagctgc tctcgcgtgt cgaaagcggc
      661 aggttgccca aggtttccgc cgtgttccac cagggcgcct gctcgaccac caccgagtgg
      721 aacggcaagt tcatgatgga cgtgaactac cgctactcgc aggcgctgct cgcgtactgc
      781 gcggcgaaga agatcccgta catctacgcc tccagcgcgt cggtctatgg catgggccac
      841 gatggtttcc gcgaagaccg ggcctgcgaa aacccgctca acatgtatgc ctactccaag
      901 ttcctgttcg accagtacgt gcgccgcaac gtgctggcga agaagcgcgc caagacagta
      961 agaggagatg cgacgcaggt ggtggggctg cgttacttca acgtctacgg tccgcgcgag
     1021 gcgcacaagg gcggcatggc cagcaccgcg ttccacttca acaaccagct caaggccgac
     1081 ggcgagtgcc ggttgttcac cggcagcgac ggctacgccg acggcgagca gcgccgcgac
     1141 ttcgtgcatg tcgacgacgt ggtatcgatg aacctgtggt tccacgatca cccggccaag
     1201 tccggcatct acaacgccgg caccggccgc gcgcagccgt tcaacgacgt ggcgcgcgct
     1261 gtcatcgcct ggcatggcaa gggccgcatc aagtacgtcc cgttcccgga gaagctggtc
     1321 ggccgctacc agtcgtacac gcaggccgat cttacggccc tgcgccaggc cggctacgag
     1381 cgcaagttca tgacggtgga gcagggcgtg aagaaatacc tggactggct gaatgcggcg
     1441 taatgctgcc gctgccgcgc tgctgctggc cggctgcgcg gcccacccca tccgcgattt
     1501 caccaccgac ggctgcacgc tgtttcccga cggcaactgg tgcgactgct gccgcgtgca
     1561 tgacgaggcc tactggcgcg gcggcacgca ggagcagcgc gaggcggccg acgtgaagct
     1621 gcgcgactgc gtgctggcga agagcggcga caaggcgctg gccgagacca tgtactacgg
     1681 cgtgcgcggc ggcggcgctc cggtctttcc cacctggtac cgctggggct acggctggga
     1741 atacggccgc ggctacgcgc cgctgtccgc cgacgaacag aaagctgcag acgccaaact
     1801 ggcggcgtat cacgccacgc atggcccgct gcaatgcggg gctgcgcgat gaacgatcgc
     1861 gtgctggtgg tcggcccgtc gtgggtcggc gacatggtga tggcgcagtc gctgttcatg
     1921 cgcctgcagc agctgcggcc ggattgcgag atcgacgtgc tggcgcccgg ctggtcactg
     1981 ccgctgatcg cgcgcatgcg cgaggtgcgc gccggcatcg agatgccgct gggccatggt
     2041 gcgtttggtt tttctgtgcg tcgtacgctc ggccactcgc tgcgggaaaa gaactacacc
     2101 caggcgatcg tgctgcccgg ctcgtggaaa tccgcgctgg tgccgttctt cgcgcgcatc
     2161 ccgcggcgca ccggctaccg cggcgagatg cgctacggcc tgctcaacga catccgtgcg
     2221 ctggacaaga acctgctcaa aacgacggtc cagcgcttcg tggcgctggc ggaggagggt
     2281 tctgtctccg ttgttccggc ggtgccggag ccgaggttcg cggtaaaggg ttccatcgcc
     2341 gttgcagccg catcggtgca agggcatgga accgcattgg tcgggctgct ccccggcgcc
     2401 gagtacggcc ccgccaagca atggccggcg gcgcactacg gcgagctggc gaagctgctg
     2461 gcgagacaga acgtgacctg cctggtgttc ggctcgaaga aggagcaggc gctgggcgag
     2521 gaaatccgcg tcgcgtccgg caacaccgcg gtcaacctgt gcgggcagac cacgctgacc
     2581 gaactggtcg atgcgctggc gcaatgcaag gcggtggtga ccaatgattc cggcccgatg
     2641 cacgtggccg cggccgccgg cgtgccgttg gtggcgatct acggctcgtc gacgcccgac
     2701 ttcacgccgc cgctcaccgc gcgcagccgc atccactacc tgcgcctggc ctgcagcccc
     2761 tgtttcgagc gcacctgccc gctggggcac tacaactgcc tgcgccagat cggcgccgcg
     2821 gcggtgcgcc agagcctgca cgaactggcc ggggtatgac gggcttataa tctgcccgcg
     2881 tctcggggca tcattcagcc atgtatgatg cttcgatccg gtggttctac cagtgggcgg
     2941 cggccggata atagacctac attcgcagtt ccccgccgac tgcggcccga tacaggaaga
     3001 gaaaatgagc tttgccgacc gtgatggcta catctggtat gacggtgccc tcaagccgtg
     3061 gcgcgagtgc accacgcatg tcctcaccta ttcactgcat tacggcgtcg gctgcttcga
     3121 aggtgtacgc gcgtaccaga cgcccaaggg cacggccatt ttccgcctgc aggatcacac
     3181 ccggcgcctg ctcaactcgg ccaagatcct gggcatgaag gtgccataca gcgccgaggt
     3241 gctgaatcag gcgcagctgg aagtgctgcg cgccaacaag ctcgattccg cctacctgcg
     3301 gccgatgatg ttctacggcg ccgagggcat gggcctgcgc gccgacaacc tgaagacgca
     3361 cgtgatcgtc gccgcctgga cctggggctc gtacctgggc gcggagaaca tggagcgcgg
     3421 catccgcgtg aagacatcct cgttcacccg ccaccacgtc aacgccgcca tgtgccgcgc
     3481 caaggcg
//
LOCUS       DASZZC010000511         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_807597,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000511 DASZZC010000000
VERSION     DASZZC010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807597"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VHE37_10855"
     CDS             complement(<1..739)
                     /locus_tag="VHE37_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36079.1"
                     /translation="MSLVRSIRSAVMRRMADAGARDRRRARAELARCKSGRPHEVLYF
                     HQPDDPYSQLAAQALAPLLQRYDIVLKPRVVARPTPIAIHDQQLWDAWAQRDCGLIAP
                     YYGLQWHGAMQPAENFLIDLARRILLAQEDLPGFPDIAVQVTSALQRDDRLALEALQQ
                     RQGAVIPADAERRLQSNEKLRHRLGHYLGGMFFYAGEWYWGIDRLHYLEARLDALGAR
                     RPAAPPGPSAALQRHAPDMIEAQQRITL"
     gene            828..1628
                     /locus_tag="VHE37_10860"
     CDS             828..1628
                     /locus_tag="VHE37_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVT36080.1"
                     /translation="MDYQTLLVNAADGVTRITLNRPDKLNALSLRLLAELGQALEAAA
                     SDAACRCVLLTGAGRGFSAGADLTDTGGTPPTKPGVLFDFEAALENGYHPVFRLLRGM
                     EKPVVAAVNGTAAGAGCNLALAADFTVATRSARFIQSFIKIGLVPDAGGTWTIPRLIG
                     RARALRWMMSGESIDAETAERWGLLAAVYDDADFAREAEALALRLAKQPTRALAGIKQ
                     LVDASLAGEFDAQLRREAQMQGKVGVSKDTMEGIMAFIQKREARFTGK"
     gene            1800..2402
                     /locus_tag="VHE37_10865"
     CDS             1800..2402
                     /locus_tag="VHE37_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36081.1"
                     /translation="MSATMLVWLAAAALYALFHFWYVGFRSKLTPEEIERVLAVVREH
                     DPSKQAMFRRIFEEDTGREFYMVNLLKLHRQLPSGEYGPQVLMKYQKPFMAAMLRRGG
                     HPIAMARAASPATESWGIDHADDWDAAVLVRYRSRRDLAEILCTPMFRSQHPYKLQAV
                     SKTFAFMADPARIIIGGPRLVVPLALLCAAALLTLAMAHV"
BASE COUNT          402 a          810 c          814 g          385 t
ORIGIN      
        1 ccagcgtgat gcgctgctgc gcttcgatca tgtccggcgc atgccgctgc aacgccgcgc
       61 tcgggcccgg gggcgcagcg ggccgccggg cgcccagcgc atcgagccgt gcttccaggt
      121 aatgcaggcg gtcgatgccc cagtaccact cgccggcgta gaaaaacatg ccgccgaggt
      181 agtggccgag gcgatggcgc agcttttcat tcgactgcag gcgccgctcg gcatcggcag
      241 gaatcaccgc gccctgccgc tgctgcagcg cttccagcgc gaggcggtcg tcgcgctgca
      301 gggccgacgt cacctgcacg gcgatgtcgg ggaacccggg caggtcctcc tgggccagca
      361 ggatgcggcg cgccaggtcg atgaggaagt tctcggccgg ctgcatcgcg ccatgccatt
      421 gcagcccgta atacggcgcg atcaggccgc agtcgcgctg cgcccaggcg tcccacagct
      481 gctggtcatg gatggcgatg ggcgtgggcc gcgccaccac gcgcggcttc agcacgatgt
      541 cgtagcgctg cagcaacggc gccagcgcct gcgcggcgag ctggctgtag ggatcgtccg
      601 gctggtggaa ataaagcacc tcgtgcgggc gcccggactt gcagcgcgcc agttccgccc
      661 gcgcgcggcg gcgatcgcgc gcgccggcgt cggccatgcg gcgcatcacc gcgctgcgta
      721 tgctgcgcac cagcgacatc ggcggccctc cccctaatcg acgcagattt tacagtatgt
      781 aaatttttgc gatagtgtac gcccccgcgc acccggaaac cgcatccatg gactaccaga
      841 ccctgctggt caacgccgcc gacggcgtca cccgcatcac gctcaaccgg ccggacaagc
      901 tcaatgcgct gtcgctcagg ctgctggcgg aactgggcca ggcgctggaa gccgcggcca
      961 gcgatgccgc ctgccgctgc gtactgctca ccggcgccgg ccgtggcttc tcggccggtg
     1021 ctgacctgac cgataccggc ggcacgccgc cgaccaagcc gggcgtgctg tttgatttcg
     1081 aggccgcgct ggagaacggc taccacccgg tgttccggct gctgcgcggc atggagaagc
     1141 cggtggtggc ggcggtcaac ggcaccgcgg ccggcgccgg ctgcaacctg gcgctggcag
     1201 ctgacttcac cgtggccacg cgctcggcca ggttcatcca gtccttcatc aagatcggcc
     1261 tggtgcccga cgccggcggc acctggacca tcccgcgcct gatcggccgc gcccgcgcgc
     1321 tgcgctggat gatgagcgga gagagcatcg acgcggaaac ggccgagcgc tggggtctgc
     1381 tcgcagcggt ttatgacgat gccgactttg cgcgcgaagc cgaggcgctg gcgctgcgcc
     1441 tggcgaagca gccgacacgc gcgctggccg gcatcaagca gctggtcgac gccagcctgg
     1501 cgggcgagtt cgacgcgcag ctcaggcgcg aggcgcagat gcagggcaag gtcggcgtgt
     1561 cgaaggacac catggaaggc atcatggcct tcatccagaa acgcgaggcg cgattcaccg
     1621 gcaagtagcg gttcgcgcag gcggattttc agggcaaccg caccctgctt tgtcctgtta
     1681 tattttattt gttaaatgtt aaaacgtaca atatttaata acatctaaaa caggggtgcg
     1741 gtgaccatga aaaaccacct gggtgcacac aacacgcagg ccgccggtgc gctgcaatca
     1801 tgagcgcgac gatgctggtg tggctggccg ccgccgcact ctatgcgctg ttccatttct
     1861 ggtacgtggg cttccgcagc aagctgacgc cggaagagat cgagcgcgta ctggccgtgg
     1921 tgcgcgaaca cgatccgtcg aagcaggcga tgttccgcag gatcttcgag gaggacaccg
     1981 gccgcgagtt ctacatggtc aacctgctca agctgcaccg ccagctgccg tcgggcgaat
     2041 acgggccaca ggtgctgatg aagtaccaga agccgttcat ggcggcgatg ctgcgccgtg
     2101 gcggtcaccc gatcgccatg gcgcgcgccg cctcgccggc cacggaaagc tggggcatcg
     2161 accatgccga cgactgggac gccgccgtgc tggtgcgcta ccgcagccgg cgcgacctgg
     2221 ccgagatcct gtgcacgccg atgttccgca gccagcatcc atataagctc caggccgtga
     2281 gcaagacctt cgccttcatg gccgacccgg cgcgcatcat catcggcggg cccaggctgg
     2341 tggtgccgct ggccctgctg tgcgccgccg cgctgctgac gctggcaatg gcccacgttt
     2401 agaatttgac a
//
LOCUS       DASZZC010000512         6335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1068790,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000512 DASZZC010000000
VERSION     DASZZC010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068790"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1493
                     /locus_tag="VHE37_10870"
     CDS             150..1493
                     /locus_tag="VHE37_10870"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HVT36082.1"
                     /translation="MQRAAAALLLPLSALAASSSFYDAPFERRPAVAELTALGRALFS
                     DPSLSASGKLSCAGCHDPAHAYGPPDGASVRPGGALGGSLGTRAVPSLRYLQKVPAFS
                     EHYTEDEGPRVGQDQGPAGGYTWDGRAATTHDQARLPLFSPLEMANAGEDDVVRHLQR
                     SAHAQQFRSAFGAHVFEQPALAFKGLLLALEVFQQDPAEFYPYSSKYDAYLRGQAQLS
                     PAEARGLQLFNDPRKGNCANCHISSMEQGAFPAFSDFGFIALGVPRNPAIPANRDAAY
                     HDLGLCGPARTDLAAHSEFCGLFRTPSLRNVSMRRVYFHNGAMHDLRKVLEFYVQRDT
                     QPQRWYPRDAHGRVNRYDDLPVQFHGNVNQEPPFGRHAGDQPALTAKEIDDVLAFLGT
                     LSDGYAARPLTDHQDRNARCADASCCSANPPSTATACSPRAASSAANASRNTAAR"
     gene            1433..1846
                     /locus_tag="VHE37_10875"
     CDS             1433..1846
                     /locus_tag="VHE37_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HVT36083.1"
                     /translation="MFAARRIKRGERIAQYRGKVMTHAQADRRPGNDESTGHTFLFTL
                     NERYVIDGAQGGNIARWINHSCAPNCEPVHLEDEDDPRQDQIVIEAIRDIEAGEELGY
                     DYGIPVEGRVTAEEKKAWACHCGARRCKGTMLRRW"
     gene            1878..3038
                     /locus_tag="VHE37_10880"
     CDS             1878..3038
                     /locus_tag="VHE37_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT36084.1"
                     /translation="MQNLPRGVVALGFVSLFMDASSESIHSLLPLFLTGVLGASALEV
                     GLISGFAEATVSFTKLFSGVVSDWIGRRKPLVLIGYGLAAATKPFFPLAHSVSTVFIA
                     HVIDRFGKGIRGAPRDALVADITPPEQRGAAYGLRQGMDTAGAFIGPLAALLLLAASH
                     NDIRYVFSWALLPALLAVLTIVLFIREPERKAADGKRGFPIRREALRRLPRAYWQVVL
                     VAALLTLARISESFLILRGQDAGLPLAWTPLVLIAMNASYALSSYPLGALADRMPRAR
                     LLMLGVVLLIAAQLLLAAGGLATAFAGIVVWGLHMGATQGLLSALVADSAPADLRGSA
                     FGMFNLVTGTSALVAGTTAGIAWTAAGPVAPFLLGAAFSMVTLVLLGAVSRR"
     gene            complement(3044..3805)
                     /locus_tag="VHE37_10885"
     CDS             complement(3044..3805)
                     /locus_tag="VHE37_10885"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HVT36085.1"
                     /translation="MNDSSRQRREAVRQLFAEAGVTQCNFLCASFRSGSTYIASLLGN
                     NGVRGLGYERLNFFVRDKIPHASDEKIIAAWERVIKESSADGVFTAKLMWGQWSAALR
                     SLNFPLQVTIDELREVFPSPRFVYNKRLDVFSQAVSYWKAQTLNVWHDTGNSDEPEPE
                     LPYEFGPIAKCLRDQLISARMWELWLAPIPHETVVYEEFIKDPATHLQRMCTIYGLER
                     GGELKLETKHRKQSNSHSKLIRAKFIEDIYQGRRL"
     gene            complement(3819..4841)
                     /locus_tag="VHE37_10890"
     CDS             complement(3819..4841)
                     /locus_tag="VHE37_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36086.1"
                     /translation="MSKPNGTLWLHIGTEKTGTTSIQRFLHDNRKRLLQAGVFVPSSL
                     GHENHKMLAAYALELNSRDIALRSQNVSNSPADVMAFQAKVRKKFTDEVQAARFEHCV
                     VSTEDLQRLYQDSEINRLFALLRPLFARIVVVLYLRRQDVLATSRRYTLLLHGDTNSD
                     VFPKKSDNFYDYHSLVTRWTRQVADDDMCIVQYRERVEGVKFDALTSFCEVIGIPLEG
                     MVMPKLLNVSLDATNQLVMLALNRQGDTVDPALRKFVLDQLGKYSDPSAHFVSRRQAE
                     EFYQRFAAGNDQLAQRFLKREQLFSTDFSMYPEENQYRKVSEAAMSRLLEILAASRAA
                     AAPPPA"
     gene            complement(4892..5305)
                     /locus_tag="VHE37_10895"
     CDS             complement(4892..5305)
                     /locus_tag="VHE37_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36087.1"
                     /translation="MTVETNLLQYAMLLGGVPETDRDRAQELAHNLGVEEEIIAHLIL
                     KRGAFAQWTRLQPLLRRAAATVNDGAATRREKGRGDFRDTTARRNEVYAAMTALIKQD
                     LGEREVRSRLDQALTEALESGATPGIAERAHIRNG"
     gene            complement(5302..>6335)
                     /locus_tag="VHE37_10900"
     CDS             complement(5302..>6335)
                     /locus_tag="VHE37_10900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVT36088.1"
                     /translation="VAERYDIPDNLAAWNERVMRELTYLKSFGLQLVSFPIWDLEALP
                     AMDDPELVPVVSLHTTYKMAKPFKPEWSLRPLYEFNMVERVIAAERQLLQRAPRILAN
                     SNAIVDILQREYGVDLSNRHEVIAHGTDDPLALPERAALAQQRDEQMLAGDKLRVLYV
                     GRFEPRKGFDIAAAVAEKLLAHGNIEMRFVGDKLSATWERRLRLGGSKLDQSDNLRFL
                     GVVERTELDDAYAEADLVLTPSRFESFGLVAIEAMAAGRPVLALNAGGLGEVVNNGVD
                     GLNWEDGDGVADSISEAIIALDHDRARLHQLGRAARASFEEKFTTGRMVEGIERFYRE
                     SLEQGRKAA"
BASE COUNT          958 a         2070 c         2135 g         1172 t
ORIGIN      
        1 tctcccagga ctggttggtg taggccccgg attctcggca gcgtctgtga cagcggcttt
       61 gccgattcgt ggcaacgtgc gtggcgttcg cgcgtcatcg ccctgccatg tcgtgctgtt
      121 atcgaagcgg gatgtccggg aaaattttct tgcagcgcgc tgctgctgcg ctgctgttgc
      181 cgctgtcggc gctcgccgcc tcatcttctt tttatgatgc gccgttcgag cgccgcccgg
      241 ccgtggccga gctgacggcg ctggggcgcg cgctgttttc cgatccgtcg ttgtccgctt
      301 ccggcaagtt gtcctgcgcg ggttgccacg atcctgcgca tgcctacggc ccgcccgacg
      361 gcgcctcggt gcgcccgggg ggcgcgctgg gcggcagcct gggcacgcgc gcggtgccat
      421 cgctgcgcta cctgcagaag gtgccggcgt tctccgagca ctacacggag gacgagggtc
      481 cgcgcgtcgg ccaggaccag ggaccggccg gcggctacac ctgggatggc cgcgctgcga
      541 ccacccatga ccaggcacgc ctgccgctgt tctcgccgct ggagatggcc aacgccggcg
      601 aagacgacgt ggtgcggcac cttcagcgca gcgcccatgc gcagcagttc cgttccgctt
      661 tcggcgcgca cgtgttcgag cagccggcgc tggcgttcaa gggcctgctg ctggcgctgg
      721 aagtgttcca gcaggacccg gcggagttct atccgtacag cagcaagtac gacgcctacc
      781 tgcgcggcca ggcgcagctg tcgccggccg aagcgcgcgg gctgcagctg ttcaacgatc
      841 cgcgcaaggg caattgcgcc aactgccaca tcagttccat ggagcagggc gcgttcccgg
      901 cgttcagcga cttcggcttc atagcgctgg gcgtgccgcg caacccggcg atcccggcca
      961 accgcgatgc cgcgtaccac gatctgggcc tgtgcggccc ggcgcgcacc gatcttgcgg
     1021 cgcacagcga attctgcgga ctgttccgca cgcccagcct gcgcaacgtg agcatgcgcc
     1081 gggtgtactt ccacaacggt gcgatgcacg acctgcgcaa ggtgctcgag ttctacgtgc
     1141 agcgcgacac ccagccgcag cgctggtacc cgcgcgatgc tcacgggcgg gtgaaccgct
     1201 acgacgatct gccggtccag tttcacggca acgtgaacca ggaacctccg ttcggacgcc
     1261 atgccggcga tcagcccgcg ctgaccgcga aggagatcga tgatgtgctg gcctttctcg
     1321 gcaccctcag cgacggctat gctgcgcgcc ctttgaccga ccaccaggac cgcaacgccc
     1381 gatgcgccga cgcaagctgc tgctccgcaa atccgccatc cacggcaacg gcgtgttcgc
     1441 cgcgcgccgc atcaagcgcg gcgaacgcat cgcgcaatac cgcggcaagg tgatgaccca
     1501 tgcgcaggcc gaccgccggc cgggcaacga cgagagcacc ggacatacct tcctgttcac
     1561 gctcaacgag cgctacgtga tcgacggcgc gcagggcggc aacatcgcgc gctggatcaa
     1621 ccacagctgc gcgcccaact gcgagccggt gcacctggag gacgaagacg atccgcgcca
     1681 ggaccagatc gtgatcgagg cgatccgcga catcgaggcg ggcgaggaac tgggctacga
     1741 ctacggcatc ccggtcgagg gccgcgtcac cgccgaggag aaaaaggcct gggcctgcca
     1801 ctgcggcgcg cggcgctgca agggcaccat gctgcggcgc tggtaatctg cgcgctgccc
     1861 gtacgctgga gtcctccatg cagaacctgc cgcgcggcgt ggtcgcgctg ggcttcgtca
     1921 gcctgttcat ggatgcgtcg agcgagagca tccactcgct gctgccgctg ttcctcaccg
     1981 gggtgctcgg cgccagcgcg ctcgaggtcg gcctgatttc gggttttgcc gaagccacgg
     2041 tgtcgttcac caagctgttc tccggcgtgg tcagcgactg gatcggccgg cgcaagccgc
     2101 tggtgctgat cggctacggc ctggccgcgg cgaccaagcc gttcttcccg ctggcgcaca
     2161 gcgtgtcgac cgtgttcatc gcgcacgtga tcgaccgttt cggcaagggc atccgcggcg
     2221 cgccgcgcga tgcgctggtg gccgacatca cgccgcccga acagcgcggc gccgcgtatg
     2281 gattgcgcca gggcatggac accgcgggcg ccttcatcgg cccgctggcc gcactgctgc
     2341 tgctcgccgc cagccacaac gacatccgct acgtcttctc gtgggcgctg ctgccggcgc
     2401 tgctggcggt gctgaccatc gtgctgttca tccgtgagcc ggagcgcaag gcggcggacg
     2461 gcaagcgtgg atttccgatc cgccgcgagg cgcttcgccg gctgccgcgc gcctactggc
     2521 aggtggtgct ggtcgccgcg ctgctgacgc tggcgcgcat cagcgaatcg ttcctgatcc
     2581 tgcgcgggca ggacgccggg ctgccgctgg cctggacgcc gctggtgctg atcgcaatga
     2641 acgcgtcgta cgcgttgtcg tcgtatccgc ttggcgcact ggccgaccgc atgccgcgcg
     2701 cgcgcctgct gatgctgggc gttgtcttgc tgatcgctgc gcagctgctg cttgccgccg
     2761 gtgggctcgc cacagcgttt gccggcattg tcgtctgggg tctacacatg ggcgcgaccc
     2821 agggcctgct gtctgcactg gtggccgaca gcgcgccggc cgatctgcgc ggcagtgcat
     2881 ttggcatgtt caacctggtg accggaactt cggccctggt ggccggcaca accgccggca
     2941 tcgcctggac ggctgccggt ccggttgcgc cgttcctgct gggagccgct ttttcgatgg
     3001 tgacgctggt gctgcttgga gcagtcagcc gtcggtaaat gagctacagg cggcggccct
     3061 gatagatgtc ttcgatgaac tttgcccgga tcagcttgga atgcgaattg ctctgcttgc
     3121 ggtgcttggt ttccagcttc aattcgccgc ctcgctcgag gccgtagatc gtgcacatgc
     3181 gttgcaggtg agtcgcagga tccttgatga attcttcgta gaccaccgtt tcatggggga
     3241 tcggcgccag ccatagctcc cacatccggg ccgagatcag ctggtcgcgc aggcatttgg
     3301 cgatcggccc gaattcgtag ggcaattcag gctccggttc atcggaattg ccggtgtcgt
     3361 gccagacatt gagcgtctgt gctttccagt agctgacggc ctgggaaaat acgtcgagcc
     3421 gcttgttgta gacgaagcgt ggactcggga agacttcacg taattcgtcg atggtcacct
     3481 gcagcggaaa attcagcgac cgcagggcgg ccgaccattg gccccacatc agcttcgcgg
     3541 tgaaaacgcc gtccgcagag ctttccttga tgacccgctc ccacgccgcg atgatttttt
     3601 cgtcgctggc gtgcgggatc ttgtcgcgca caaaaaaatt aaggcgctcg taacccaggc
     3661 cgcgcacgcc attgttgccg agcagtgacg caatgtaggt gcttccgcta cggaacgacg
     3721 cgcacaggaa attgcactgg gtgacgccgg cttcggcgaa caactggcgc acggcttcgc
     3781 ggcgctggcg ggaggaatcg ttcatcggat gaattggatc aggcaggtgg aggcgcggcc
     3841 gccgcgcgcg aagccgcgag gatttccagc agccgcgaca tggccgcttc actgaccttg
     3901 cgatactggt tttcctccgg gtacatggag aaatcggtgg aaaacagctg ctcgcgcttg
     3961 aggaagcgct gcgccagctg gtcgttgccg gctgcgaagc gctggtagaa ctcttcggcc
     4021 tgcctgcgcg atacgaaatg cgcggaagga tcgctgtact tgccgagctg atcgagcacg
     4081 aatttgcgca gcgccgggtc gaccgtgtcg ccctggcggt tgagcgccag catcaccagc
     4141 tggttggtgg cgtcaaggct gacgttgagc agcttgggca tgaccatgcc ttccagcgga
     4201 atgccgatca cttcgcagaa gctggtcagg gcatcgaact tgaccccttc gacgcgttcg
     4261 cggtactgca cgatgcacat gtcatcatcg gcgacctgcc gcgtccagcg cgtcaccagc
     4321 gaatggtagt cgtagaagtt gtcgctcttc ttcgggaaca catcgctgtt ggtgtcgccg
     4381 tgcagcagca gggtgtagcg ccggctggtg gccagcacgt cctggcggcg caggtagagc
     4441 accaccacga tgcgggcaaa cagcggccgc aggagcgcga acaggcggtt gatctcgctg
     4501 tcctggtaca gccgctgcag gtcctcggtc gagaccacgc agtgctcgaa gcgggcggcc
     4561 tgcacctcgt cggtgaattt cttccgcacc ttggcctgga aggccatgac gtcggcgggc
     4621 gagttggaga cattctgcga gcgcagcgcg atgtcgcgcg aattcagctc cagtgcatag
     4681 gccgccagca tcttgtggtt ctcgtgcccc agcgaactgg gcacgaagac gccggcctgc
     4741 agcagccgct tgcggttgtc gtggaggaag cgctggatcg acgtggtgcc ggtcttttcg
     4801 gtgccgatgt gcagccagag cgtcccgttg ggtttggaca tagtgcgcct gtcattctag
     4861 ctgccgcagc gcggcagcgg ggaatgcatg atcagccgtt gcggatgtgg gcgcgctcgg
     4921 caatgcctgg cgtggcgccg gattcgagcg cttcggtcaa ggcctggtcc aggcggctgc
     4981 gcacttcgcg ttcgcccagg tcctgcttga tcagggcggt catcgccgca tagacctcgt
     5041 tgcgccgtgc cgtggtgtcg cggaaatcgc cgcggccttt ttcgcggcgg gtggcggcgc
     5101 cgtcattgac cgtcgcggcg gcgcgcctga gcagaggctg cagcctggtc cattgggcaa
     5161 aggcgccgcg cttgaggatc aggtgcgcaa tgatctcctc ctcgacgccc aggttgtggg
     5221 ccagttcctg cgcgcggtcg cggtcggttt cgggcacgcc gcccagcagc atcgcgtact
     5281 gcaacaggtt tgtttcgacc gtcatgcggc cttcctccct tgctccaggc tctcgcggta
     5341 gaagcgttcg atgccttcca ccatccggcc ggtggtgaat ttttcctcga agctggcgcg
     5401 cgcggcgcgg cccagctggt gcaggcgggc gcgatcgtgg tccagcgcga tgatcgcctc
     5461 gctgatgctg tcggccacgc cgtcgccgtc ctcccagttg aggccgtcga ccccattgtt
     5521 gaccacctcg cccaggccgc cggcattcag cgccagcacc ggccggccgg cggccatcgc
     5581 ctcgatggcc accaggccga acgactcgaa gcggctgggc gtcagtacca ggtcggcctc
     5641 ggcataggca tcatccagct cggtgcgctc caccacgccc aggaagcgca ggttgtccga
     5701 ctggtcgagc ttgctgccgc ccagccgcag gcggcgttcc caggtggcgc tgagcttgtc
     5761 gccgacgaag cgcatctcga tgttgccgtg ggccagcagt ttttccgcca cggctgcggc
     5821 gatgtcgaag cctttgcgcg gctcgaagcg gccgacatag agcacgcgca gcttgtcgcc
     5881 ggccagcatc tgctcgtcgc gctgctgcgc cagcgcggcg cgctcgggca gcgccagcgg
     5941 atcgtcggtg ccgtgggcga tcacttcgtg gcggttggac aggtcgacgc cgtattcacg
     6001 ctgcaggatg tcgacgatgg cgttgctgtt ggccaggatg cgcggcgcac gctgcagcag
     6061 ctgccgctct gccgcgatca cccgctccac catgttgaac tcgtacagcg ggcgcaggct
     6121 ccattccggt ttgaacggct tggccatctt gtaggtggtg tgcaggctga ccaccggcac
     6181 cagctcggga tcgtccatcg ccggcagcgc ttccaggtcc cagatgggga atgacaccag
     6241 ctgcaggccg aaggacttga ggtaagtgag ctcgcgcatg acgcgctcgt tccaggccgc
     6301 caggttgtcc gggatgtcgt agcgctcggc caccg
//
LOCUS       DASZZC010000513         8277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_867943,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000513 DASZZC010000000
VERSION     DASZZC010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867943"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VHE37_10905"
     CDS             <1..599
                     /locus_tag="VHE37_10905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT36089.1"
                     /translation="GVTPVKKSELIAGATDETPVLFMAGRLSKEKGILELPEIYRRAR
                     KEIPDLRIVIAGIGPAEAELRELLPEATYLGWINRERIEQMYAGLDLFVFPSKFDTFG
                     NVVLEAFSQGMPVAAYNTKGPKDIIENGRNGYLVDKLGEMAAQIVKHFREPQRHASMR
                     AAALRRVDDYQAGPIMREFLGRMGLPVEQPIIERRAAA"
     gene            604..1620
                     /gene="asd"
                     /locus_tag="VHE37_10910"
     CDS             604..1620
                     /gene="asd"
                     /locus_tag="VHE37_10910"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HVT36090.1"
                     /translation="MSNTPDKLHTSADAVPAVPARVRWKLRVPKPLRLPVPQRLRGRL
                     RLRPRTLNPLRRLTQQEQINFLLTNRIPRHALTKLVGRVSRIHNPKLARALIGMWRLF
                     ADDLRLHESEQQSFASLQECFTRRLKPGMRPLDEREHIAVSPCDAVIGECGRIEGSRV
                     FQAKGFPYELGELIPDPAVQQLYRDGTYVTLRLKSDMYHRFHAPVDCRTQQITYISGD
                     TWNVNPIALKRVEKLYCRNERAVVQLHDAATGGSLCLVPVAAILVASMRFAGLHDELS
                     LAYRGPNLIPWQRSFAKGEEMGWFQHGSTILLFATGNYALCEGIRSGDTIRMGQALLT
                     ASSV"
     gene            1637..2269
                     /locus_tag="VHE37_10915"
     CDS             1637..2269
                     /locus_tag="VHE37_10915"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HVT36091.1"
                     /translation="MNFADELHQQRWDDHRYYHHNRVNQALHLLSACCFLVSYALIFV
                     NPVWAVMAAWPAAMIVRQIGHFFFEPKTYDDVNQATHEYKESVKVGYNLQRKVVLLSI
                     WGAAPVVLYFQPAFFGFFSADEVARGFLYNLSILWLIIGIGAVIFRTVHLFFLMGVQS
                     GLVWATKILTDPFHDIKIYHKAPYYIAKGQMYDDMTEWYRTPPPAQTAKA"
     gene            complement(2290..3030)
                     /locus_tag="VHE37_10920"
     CDS             complement(2290..3030)
                     /locus_tag="VHE37_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36092.1"
                     /translation="MNAEGSLPAVAAVTLVLLPGLDGTDVFFRPLLSRLPAGIRALPL
                     TLPDHGPWSYAGLLEVMRRELADVPRCYVLAASFGGPLAVLLAQAEPHKLRGLILTAT
                     FLRSPRPRLSPLRWLLRTPVVWSLRAARRLPLLLRRRSGDALRVAKAETWQRTSARSL
                     AARARAALAVDVREAMSAVQMPVLAVSYAADSVVSAAATREILDRCRDARNVTLDGGH
                     LAMFSDPQPLAEQISRFIEACEAPMKRR"
     gene            complement(3326..4819)
                     /locus_tag="VHE37_10925"
     CDS             complement(3326..4819)
                     /locus_tag="VHE37_10925"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HVT36093.1"
                     /translation="MGRAYDHLATHFDLIRRLEGAGSLLFWDRAVVMRPGSAPAHARI
                     AAGLAQVITEKETDPRIAGWLSESDEERPALDHWKRANLDEMRRRHRHATAVPLALQV
                     RKAEAAAALNPVWQRARADNDFAAFAPGLREMIKLQQECAAYKAEALGLSPYDALMDE
                     HDPGVTCAMVDGVFGPLSRELPRLLEQALARQSAWKPLPLAAVHAADAQRELCELLMK
                     QVGYDFDCGRLDTTAPPFAMPRVPGDTRITTRYKEDDLRFALMATLHETGHSMYEYNL
                     PRVWGWQPVGLARGATLHESQSLMLEMVACRSPEFVAFVAPLLAARFGAQDPAYRYDN
                     LLLHYHRVQRNFIRIEADQIVYPLHVILRYDIERALLDNRLDVADLPQAWNEKMRELI
                     GVVPPSDALGCLQDVHWAFGMFGYFPNYAMGAATAAQLFEAATRADPGILPSIAKGDF
                     KPYYAWVKPNVHAKASSEPLARIIEQATGAPLSADALLRHLRQRYLG"
     gene            5079..5753
                     /locus_tag="VHE37_10930"
     CDS             5079..5753
                     /locus_tag="VHE37_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36094.1"
                     /translation="MPASPPQPGRPRNTSRERILDMALHLLDHRGPQALTLSTLAAEL
                     AMGISSLYTYFSDLAELEDEVAARLIGQVPALDAARGPLRDQLLAFGAAILRMHSVHP
                     YLRLIAGRASAVVGARLRRANLAVLMASGVEAARANDCLEMIHALAYSGGSELHRLQQ
                     LSAGVRKAVDKIVADEVGADLVRAAAGDLSVAARHAHLFERCIDSLLPEAGQAPGARR
                     RGRRAR"
     gene            5743..8085
                     /locus_tag="VHE37_10935"
     CDS             5743..8085
                     /locus_tag="VHE37_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36095.1"
                     /translation="MRASAAVRLLAATLLACSACVHADDVPADAQPAASTDPATIPVA
                     EPPQEAAPAPAASGNEPPQHLEDIVVTATKREQSVRKIAGTVNALHGEDLEKIGAREL
                     TDYINQVPGITLAEGEYGYNRNFAIRGVGPGDGGNQTTGQFIGDIPLTDPGQTVVLPD
                     LDPFDLRTFEVLKGPQGTTFGATALNGAIRFVPNAPVLGAWEARGFADELGVDQGGRG
                     LTYAGALNAPVGDSLALRGVGVMQHVPGVVDNLQRGTPDADSRRKWSARGMALWQPLE
                     RLTINGLFLKQQSHQNDLGASDNDQGRRENNYSPGPSTIDVNFALAALDLRYAFDGST
                     LVWQSSRSAKSLYAQFDTSRAVEFLANQGVQNLAALATADIVSYTHELRLVSPNEGPW
                     KWTAGLFLQRYRSDQLTDQYVASTSALGTVLSQRPGIEGLASERGISVGGSHAAPIQT
                     EERAAYGDLSRKFGERWELTLGTRYYDTRLHADVDYTGAVILLYYHTTSSLHHYDQSE
                     RGFSPKASLVFQITPDVLTYATVARGFQFGGVNTYVALVPTDSIPPTYRSSTLWSYES
                     GIRTDWFDRTLRMDLTAFYIDWSDAQLQLTTPTLVETPYFDNVGKVHSKGLEGSLAWL
                     TPVPGLSLNVAGSYILAKTAADYTNGTQHVPAGTDMGAAPHWQTAETLAYTQYFGSWI
                     TSASLNHTYTGHAWNNIFHAFDIYNYSQYGLNLSVARPDWFASPSLALGITNLTNVDA
                     VIGHSQAGAATAAGNVRATGPNVIYNRPRAINLRLSAEFR"
     gene            8090..>8277
                     /locus_tag="VHE37_10940"
     CDS             8090..>8277
                     /locus_tag="VHE37_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36096.1"
                     /translation="MPYATAPDGAQLYYEVHGSGPFLFIGPSPGARITPIRLLPRRLG
                     PRPYIEAFSGDYRVIVMD"
BASE COUNT         1382 a         2879 c         2759 g         1257 t
ORIGIN      
        1 acggcgtgac gccggtgaag aaatcggaac tgatcgccgg cgccaccgat gaaacgccgg
       61 tactgttcat ggccggccgc ctgtcgaaag agaagggcat cctcgagctg ccggaaatct
      121 accgccgcgc gcgcaaggaa atcccggacc tgcgcatcgt catcgccggc attggtccgg
      181 ccgaggcgga gctgcgcgag ctgctgcccg aggcgacgta cctgggctgg atcaaccgcg
      241 agcgcatcga gcagatgtac gccgggctcg acctgttcgt gttcccgtcg aagttcgaca
      301 ccttcggcaa cgtggtgctc gaggcgtttt cgcagggcat gccggtggcg gcctacaaca
      361 ccaagggccc caaggacatc atcgagaacg ggcgcaacgg ctacctggtc gacaagctcg
      421 gcgagatggc cgcgcagatc gtgaagcact tccgcgagcc gcaacgccac gcctcgatgc
      481 gcgcggccgc actgcgccgc gtggacgact accaggccgg gccgatcatg cgcgagttcc
      541 tcggccggat gggcctgccg gtcgagcagc ccatcatcga gcggcgcgcg gccgcatgac
      601 gccatgagca acacgcccga caagctgcac acttcagcgg acgccgtgcc ggcagtgccg
      661 gcgcgcgtgc gctggaagct gcgcgtgccc aagccgctgc gcctgccggt gccccagcgc
      721 ctgcgcggcc gcctgcgcct gcgcccgcgc acgctcaacc cgctgcgccg gctgacgcag
      781 caggagcaga tcaacttcct gctgaccaac cgcatcccgc gccacgcgct gaccaagctg
      841 gtggggcgcg tcagccgcat ccacaacccg aagctggcgc gcgcgctgat cggcatgtgg
      901 cgcctgttcg ccgacgacct gcgcctgcac gagtccgagc agcagagctt tgccagcctg
      961 caggaatgct tcacccgccg gctcaagccg ggcatgcgcc cgctcgacga gcgcgagcac
     1021 atcgcggtca gcccctgcga tgcggtgatc ggcgaatgcg gccgcatcga gggcagccgc
     1081 gtgttccagg ccaagggttt cccgtacgaa ctgggcgagc tgattcccga cccggcggtg
     1141 cagcagctgt accgcgacgg cacctacgtg acgctgcgcc tgaaatcgga catgtatcac
     1201 cgcttccacg cgccggtgga ctgccgcacg cagcagatca cctacatctc gggcgacacc
     1261 tggaacgtca accccatcgc gctcaagcgc gtggaaaagc tctactgccg caacgagcgc
     1321 gccgtggtgc agctgcacga cgccgctacc ggcggcagcc tgtgcctggt gccggtggcc
     1381 gccatcctgg tggcgagcat gcgcttcgcc ggcctgcacg acgagctcag cctggcctac
     1441 cgcggcccca acctgatccc gtggcaacgc agctttgcca agggcgagga gatgggctgg
     1501 ttccagcacg gctccaccat cctcctgttt gccaccggca actacgcgct gtgcgaaggc
     1561 atccgcagcg gcgacaccat ccgcatgggg caggccttgc tcactgcgtc ttcagtctga
     1621 tccaaggagt acagccatga actttgccga cgaattgcac cagcagcgct gggacgatca
     1681 tcgctattac caccacaacc gcgtcaacca ggcgctgcac ctgctgtccg cgtgctgctt
     1741 cctggtcagc tacgcgctga tcttcgtcaa cccggtgtgg gcggtgatgg ccgcctggcc
     1801 ggcggcgatg atcgtgcgcc agatcggcca cttcttcttc gagccgaaga cctacgacga
     1861 cgtcaaccag gccacgcacg agtacaagga aagcgtgaag gtgggctaca acctgcagcg
     1921 caaggtggtg ctgctgtcga tctggggagc ggcgccggtg gtgctgtatt tccagccggc
     1981 cttcttcggc ttcttcagcg ccgacgaagt ggcacgcggc ttcctctaca acctgtccat
     2041 cctgtggctg atcatcggca tcggcgccgt gatcttccgc accgtgcacc tgttcttcct
     2101 gatgggcgtg cagtccggcc tggtgtgggc gaccaagatc ctcaccgacc cgttccatga
     2161 catcaagatc taccacaagg cgccgtacta catcgccaag gggcagatgt acgacgacat
     2221 gaccgagtgg taccgcacgc cgccgccggc acagacggcc aaggcctgat cgccggttga
     2281 gcgacgccct caacgccgct tcatcggggc ctcgcaggcc tcgatgaagc ggctgatctg
     2341 ctcggccagc ggctgcggat cgctgaacat ggccaggtgc ccgccgtcga gcgtgacgtt
     2401 gcgcgcatcg cggcagcggt caaggatttc gcgcgtggca gcggcgctga ccacgctgtc
     2461 ggcggcatag gacacggcca gcaccggcat ctgcaccgcg ctcatcgcct cgcgcacatc
     2521 caccgccagt gcggcgcggg cacgtgctgc caggctgcgt gccgaagtcc gttgccaggt
     2581 ttccgctttc gccacccgca gcgcgtcgcc gctgcgcctg cgcaacagca gcggcagccg
     2641 ccgcgccgcg cgcaggctcc acaccacggg cgtgcgcagc agccagcgca gcggcgaaag
     2701 ccgcgggcgg ggcgagcgca ggaaggtggc ggtgagaatc aggccacgca gcttgtgcgg
     2761 ctcggcctgc gccagcagca ccgccagcgg gccgccgaac gaagcggcca gcacgtagca
     2821 gcgcggtacg tcggccagct cgcggcgcat gacctcgagc agtcccgcgt agctccacgg
     2881 cccgtgatcc ggcagcgtca gcggcagcgc gcggatgccg gccggcagcc gcgacagcag
     2941 cgggcggaaa aacacgtcgg tgccatcgag cccgggcagc agcaccaggg tcaccgcagc
     3001 gactgcaggc aaggagccct cggcgttcat gctgtacggg ccgggatagc ggacgaagga
     3061 gaaagtcgca tcgttcggcc atcatgccgc cgccatcttg caggacgatt tccagcgcgc
     3121 aagcacttcc gcagctgcct ggaaagggtt accgcacccg gcctggcgat gttattaatt
     3181 atcttatata tgtaaaaata ataaacaatt aataacatgc ttcgcggcct tgcggtcggc
     3241 cgtttttcaa ctgccaggac tgttccgctg ccgtccgcgc cgcacttcgc cctaacaatc
     3301 tgctgcggag gaaaaagcgc cgcgctcagc ccaggtagcg ctggcgcagg tggcgcagca
     3361 gcgcgtcggc cgacagcggc gcgccggttg cctgctcgat gatccgcgcc agtggttcgc
     3421 tcgacgcctt ggcgtgcacg ttcggcttga cccaggcgta gtacggcttg aagtcgccct
     3481 tggcgatgga cggcaggatg ccgggatcgg cacgcgttgc cgcctcgaac agttgcgccg
     3541 cggtggccgc gcccatcgca tagttcggga aatagccgaa catgccgaag gcccagtgca
     3601 cgtcctgcag gcagccgagc gcatcgctgg gcggtaccac gccgatcagc tcgcgcatct
     3661 tctcgttcca ggcctgcggc aggtcggcca cgtcgagccg gttgtcgagc agcgcgcgct
     3721 cgatgtcgta gcgcaggatc acgtgcagcg ggtagacgat ctggtcggcc tcgatgcgga
     3781 tgaagttgcg ctgcacgcgg tggtaatgaa gcagcaggtt gtcgtagcgg taggccgggt
     3841 cctgggcgcc gaaacgcgcc gccagcagcg gcgccacgaa ggcgacgaat tccggcgagc
     3901 ggcacgccac catttccagc atcagcgact ggctctcgtg cagcgtggcg ccgcgcgcga
     3961 gtccgaccgg ctgccagccc catacgcggg gcaggttgta ctcgtacatc gagtggccgg
     4021 tttcgtgcag cgtcgccatc agcgcgaagc gcaggtcgtc ctccttgtag cgcgtggtga
     4081 tgcgcgtgtc gcccggcacc cgcggcatgg cgaacggagg cgcagtggtg tcgaggcggc
     4141 cgcagtcgaa gtcgtagccc acctgcttca tcagcagctc gcacagctcg cgctgcgcat
     4201 cggcggcgtg caccgccgcc agcggcagcg gcttccacgc ggactggcgc gccagcgcct
     4261 gctcgagcag gcgcggcagc tcgcgcgaca gcgggccgaa cacgccgtcg accatggcgc
     4321 aggtcacgcc gggatcgtgc tcgtccatca gcgcgtcgta cggcgacagg ccaagcgcct
     4381 cggccttgta cgcggcgcac tcctgctgca gcttgatcat ctcgcgcagg ccgggcgcga
     4441 aggcggcgaa gtcgttgtcc gcacgcgcac gctgccacac cgggttgagc gccgccgcgg
     4501 cctcggcctt gcgcacctgc agggccagcg gcacggcagt ggcgtggcga tggcggcggc
     4561 gcatttcgtc gaggttggcg cgtttccagt gatccagcgc cgggcgttcc tcatccgatt
     4621 ccgacagcca gccggcgatg cgcggatcgg tttccttctc ggtgatcacc tgcgccaggc
     4681 ccgccgcgat cctggcgtgc gccggcgccg agccggggcg catgaccacc gcgcgatccc
     4741 agaacaacag cgagccggcg ccctcgaggc ggcggatcag gtcgaaatgc gttgccagat
     4801 ggtcgtaggc gcggcccatg tttggatgct ctggtacaag acaggtttgt attgttatgg
     4861 cgaaaccgcc tgcagggcaa ctgcgcgccc gccagcgggc gcgggcggca gcggggcgcg
     4921 ccggcatcgc ggaaggtgct gttgtcggca cagggttgcc ggggccacgc ggccggcgcg
     4981 gggcaggcgg ccgtcgtgct tgacatagaa tgcatcatgg atcagtgtat tttgcatttc
     5041 cgaaagtatt tcgataatta tccatgcaag ccgccgccat gcccgccagt ccgccgcagc
     5101 ccgggcgccc gcgcaatacc agccgcgagc gcatcctcga catggcactg cacctgctcg
     5161 accatcgcgg accgcaggcg ctgaccctca gcacgctggc cgccgaactg gccatgggca
     5221 tctcgtcgct ctacacctat ttcagcgacc tggccgaact ggaggacgaa gtggctgcgc
     5281 gcctgatcgg gcaggtaccg gcgctcgacg cggcgcgcgg accgctgcgc gaccagctgc
     5341 tggcttttgg cgcggccatc ctgcgcatgc acagcgtgca cccttacctg cgcctgatcg
     5401 ccgggcgcgc cagcgcggtg gtcggcgcgc gcctgcgccg cgccaacctg gcggtgctga
     5461 tggccagcgg cgtagaggcg gcgcgcgcca acgactgcct agagatgatc catgcgctgg
     5521 cctacagcgg cggcagcgag ctgcaccgcc tgcagcagtt gagcgccggc gtgcgcaagg
     5581 cggtggacaa gatcgtcgcc gacgaggtgg gtgcggacct ggtgcgcgcc gccgcgggtg
     5641 acctgtcggt ggcggcgcgg catgcccacc tgttcgagcg ctgcatcgac agcctgctgc
     5701 ccgaagccgg gcaggcgccc ggtgcgcggc ggcggggcag acgtgcgcgc tagcgccgcg
     5761 gtgcggttgc tggccgccac gctgcttgcc tgctcggcgt gcgtgcacgc ggatgacgtg
     5821 cctgccgacg cgcagcccgc cgcgtccacc gatcccgcca ccattcccgt ggccgagccg
     5881 ccacaggagg cagcgccggc cccggcggcc tccggcaacg agccgccgca gcacctggaa
     5941 gacatcgtgg tcaccgcgac caagcgcgag cagtcggtgc gcaagatcgc cggcacggtc
     6001 aacgcgctgc atggcgaaga cctggagaag atcggcgcgc gcgagctgac cgactacatc
     6061 aaccaggtgc cgggcatcac gctggccgaa ggcgaatacg gctacaaccg caacttcgcc
     6121 atccgcggcg tggggccggg cgatggcggc aaccagacca ccggccagtt catcggcgac
     6181 attccgctga ccgatccggg gcagaccgtg gtgctgccgg atctcgatcc tttcgacctg
     6241 cgcaccttcg aagtgctgaa gggcccgcag ggcaccacct ttggcgccac tgcgctcaat
     6301 ggcgcgatcc gcttcgtgcc caacgcgccg gtgctgggtg cgtgggaagc gcgcggcttc
     6361 gccgatgagc tgggcgtgga ccagggcggc cgcggcctga cctatgccgg cgcgctcaat
     6421 gcgccggtcg gcgacagcct ggcgctgcgc ggcgttggcg tgatgcagca tgtgcccggc
     6481 gtggtcgaca acctgcagcg cggcacaccc gatgccgaca gccggcgcaa atggtcggcg
     6541 cgcggcatgg cgctgtggca gccgctggag cggctcacca tcaacggcct gttcctcaag
     6601 cagcagtcgc accagaacga cctgggcgca tcggacaacg accagggccg gcgggagaac
     6661 aactacagcc cggggccgag caccatcgat gtcaatttcg cgctggccgc actcgacctg
     6721 cgctacgcct tcgacgggtc gacgctcgtg tggcagtcat cgcgctcggc caaatcgctg
     6781 tacgcgcagt tcgacaccag ccgtgcggtg gaattcctgg ccaaccaggg cgtgcagaac
     6841 ctggcggcac tggccaccgc cgacatcgtc agctacacgc acgaactgcg gctggtctcg
     6901 cccaacgaag ggccgtggaa atggaccgcg ggcctgttcc tgcagcgcta ccgctccgac
     6961 cagctgaccg accagtacgt ggccagcacc agcgcgctgg gcacggtgtt gtcgcagcgg
     7021 ccgggcatcg aaggcctggc cagcgaacgc ggcatctcgg tgggcggctc gcatgccgcg
     7081 ccgatccaga ccgaggagcg cgccgcgtac ggtgatctgt cgcgcaagtt cggcgagcgc
     7141 tgggaactga cgctgggcac gcgctactac gacacgcggc tgcacgccga tgtcgactac
     7201 accggcgcgg tgatcctgct ctactaccac accaccagca gcctgcatca ctatgaccag
     7261 tcggagcgcg gcttcagtcc caaggcctcg ctggtgttcc agatcacgcc cgacgtgctg
     7321 acctatgcca cggtggcgcg cggtttccag ttcggcggcg tcaacaccta cgtggcgctg
     7381 gtgcccactg attcgatccc gccgacctac cgctccagca cactgtggag ttatgaaagc
     7441 ggcatccgca ccgactggtt cgaccgcacg ctgcgcatgg acctgactgc gttctacatc
     7501 gactggagcg atgcgcagct gcaactgact acgccgacgc tggtggagac gccgtatttc
     7561 gacaacgtgg gcaaggtgca cagcaagggg ctggagggat cgctggcctg gctcacgccg
     7621 gtgcccggcc tgtcgctcaa cgtggcgggg tcttacatcc tggcgaagac ggcggcggac
     7681 tacaccaacg gcacgcagca cgtgcccgcc ggcaccgaca tgggcgccgc gccgcactgg
     7741 cagacggcgg aaacgctggc ctacacgcag tacttcggca gctggatcac cagcgcgtcg
     7801 ctgaaccaca cctataccgg ccacgcctgg aacaacatct tccacgcttt cgacatctac
     7861 aactattcgc aatacggcct caacctcagc gtggcgcgtc cggactggtt cgcctcgcca
     7921 tcgctggcgc tgggcatcac caatctcacc aacgtggacg cggtgatcgg ccacagccag
     7981 gccggcgctg ccaccgcggc gggcaacgtg cgcgctaccg gccccaacgt catttacaac
     8041 cggccgcgcg ccatcaacct gcggctgtcc gcggaattca ggtagagcca tgccctatgc
     8101 caccgcgccg gacggcgcac agctgtacta cgaggttcat ggcagcggtc cgttcctgtt
     8161 catcgggccg tcgccgggtg cgcgcatcac gcccatccgc ctgctgccgc ggcgcctggg
     8221 gccgcggccg tacatcgagg cgttctcagg cgattaccgg gtcatcgtca tggacca
//
LOCUS       DASZZC010000514         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_807893,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000514 DASZZC010000000
VERSION     DASZZC010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807893"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..899
                     /locus_tag="VHE37_10945"
     CDS             141..899
                     /locus_tag="VHE37_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36097.1"
                     /translation="MRKQLGVRALVRGWSTAAVAAAAMMPAGMVRAGDDATDCPPPPP
                     PPACSARTSITSNFNGTRVPGGDYIWFNSVLKVAGLPSKPVTIRMTHATITFTANGQN
                     YTVWLPDAQVTFDSTVTQATTTYDASANSWITRTRPGLAGNTFLSGYPWLVNQTLPGG
                     TNPVTWQGEIDSDTPGVKVNWQWAAAAYSSFSSYPDTLGVKPVDDNKGSQYANSDHAG
                     TPEAFKTYVVGGARGGGGANATGSYSGTGHADCG"
     gene            987..>1574
                     /locus_tag="VHE37_10950"
     CDS             987..>1574
                     /locus_tag="VHE37_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HVT36098.1"
                     /translation="MLSEKFVDYLKLMRLHKPIGIFLLLWPTLWALWIAAGGVPPAQV
                     LFVFVAGTVLMRSAGCAINDFADREFDPQVARTRDRPVATGRVAPDEAVRLFVAVSLI
                     AFGLLTSLKNVLALGLAIPAALLAGTYPFTKRWISLPQAVLGIAFSWGIPMAYAAVQH
                     RVNWPEALQLMTINFCWVMAYDTWYALADRADDVKA"
BASE COUNT          258 a          534 c          514 g          268 t
ORIGIN      
        1 gctgatcacg cgctgggtcg gcgacgtgga gaagtacgcg gtggtgtgag cgaggctgct
       61 tttcgttgat tcccgaactg ttcagttcgc gttcagtcag tgcatttact ggcggtgcat
      121 ctgatcaagg aggatggatc atgcggaagc aacttggtgt ccgcgcgctg gtgcgcggat
      181 ggtcgacagc agccgttgca gcagcggcga tgatgcccgc gggcatggtc cgcgcgggcg
      241 acgacgccac cgattgccca ccgccgccac cgccgccggc gtgcagcgcg cggaccagca
      301 tcacctccaa tttcaacggc acccgggttc ccggagggga ctacatctgg ttcaacagcg
      361 tgctcaaggt ggctggcctg cccagcaagc cggtgaccat ccgcatgacg cacgcaacca
      421 tcaccttcac cgccaatggc cagaactaca ccgtgtggct gcccgacgcg caggtcactt
      481 tcgacagcac ggtcacgcag gccaccacga cttatgacgc ttcggccaac agctggatca
      541 cgcgcacccg gccgggcctc gccggcaaca ccttcctgtc cggctatccg tggctggtca
      601 accagacgct gccgggcggc accaacccgg tgacctggca gggcgaaatc gattccgaca
      661 cgccgggggt caaggtgaac tggcaatggg cggcggcggc gtacagcagt ttcagcagct
      721 acccggacac gctcggcgtg aaaccagtgg acgacaacaa gggcagccag tacgcgaatt
      781 ccgatcatgc cggcacgccg gaggcattca agacctacgt ggtcggcggc gcgcgcggcg
      841 gcggcggcgc caacgccacc ggctcgtata gcggcaccgg tcacgccgac tgcggctgac
      901 cgccggtcgc catccgcagc atggccgccg gcgcgaagcc ggcggccttt ttattggccg
      961 gccatgcgcc ggtgtcacaa tgacgcatgc tgtccgaaaa attcgtcgac tacctgaagc
     1021 tgatgcggct gcacaagccc atcggcattt tcctgctgct gtggcccacg ctgtgggcgt
     1081 tgtggatcgc cgcgggcggc gtacccccgg cgcaggtgct gttcgtgttc gtcgccggca
     1141 ccgtgctgat gcgctcggcc ggctgcgcca tcaacgattt tgccgaccgc gagttcgatc
     1201 cgcaggtggc gcgcacccgg gatcgtcccg tcgccaccgg ccgcgtggcg ccggacgaag
     1261 ccgtgcgcct gttcgtcgcc gtgtcgctga tcgcgttcgg cctgctcacc tcgctgaaga
     1321 acgtgctggc gctgggcctg gccattcccg ccgcgctgct cgccggcacc tatcccttca
     1381 ccaagcgctg gatcagcctg ccgcaagcgg tgcttggcat cgcgttttcc tggggcatac
     1441 cgatggccta cgcggccgtg cagcaccggg tgaattggcc cgaggcgctg cagctgatga
     1501 ccatcaactt ctgctgggtg atggcctacg acacctggta cgcgctggcc gaccgcgccg
     1561 acgatgtgaa agcc
//
LOCUS       DASZZC010000515         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_868002,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000515 DASZZC010000000
VERSION     DASZZC010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868002"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHE37_10955"
     CDS             complement(<1..407)
                     /locus_tag="VHE37_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVT36099.1"
                     /translation="MLHIFRTGLAAAVTVLLSSAALAAPEALDRIIVVVNDGVVLQSE
                     LDQALDSAHKTIAERNLQAPPDSVLRGQVLDRLVLVKLQTQRAEQAGIRVDDRELNDV
                     MTNIAQQNKVTLAEFSEQLKKQGMDFLAVREQVR"
     gene            complement(496..2676)
                     /gene="lptD"
                     /locus_tag="VHE37_10960"
     CDS             complement(496..2676)
                     /gene="lptD"
                     /locus_tag="VHE37_10960"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HVT36100.1"
                     /translation="MRLSRLFLLLLPVCAHAAVPVEEASCPQLDYSLPPLVNTGDTRV
                     HVTADQGVLEQGGLSSLAGSVRLQQGDKEFSAQQMDYDDKERQVHVRQQSLFRNQNII
                     VRSQRLDFDLDSETGVFSDTDFTLPQRAARGDAGVLTVGRNGTAEFQAVRYTTCAPGS
                     RGWYLEGRDIKLDQHAGVGTAHNVKLEFLGVPILYAPWFQFPIDNQRRTGLLFPTVGE
                     STRTGFDFRWPLYLNLGPNYDATLTPRVMSKRGLLLGSNTRYLEPESEGHLHFDYLAH
                     DAQTGEQRSYLQFDHKGLLASHLSLEAHYYDVSDQQYFEDLGGAVDATALTALDRSLL
                     LTYQAPSAYTLTLLAQDYQVVDQTVALSDQPYRRLPQIDFRVRTKNSLLDTRAGLSSE
                     YSNFLRADSVQGQRLTLQPYLLTEIEQQTWFLNSELNLDYTHYLLGATALGQPDSPTR
                     TLPQFSIGSGLRFERFTAAGSLQTLEPQVFYLYTPFRDQDQLPVFDSGDPDFDFTQLF
                     ARNRFSGEDRISDANQLAVAATSRLLNPYSGEEKLAASIGQLYRFTQPQVQLPSPTQP
                     PPSQGATDFIASLDYHLSHLWIAGFSSQWSPRDHRFNRASVAMHYRDSGRRFDVAYRY
                     RQNILEQADIAGSAPLSSAWRGTARVRYSIADHTPLDTLAGVEYSTCCWALSTSYRRY
                     ISTTRGEFNSGVYLQLSLKGLSRIGTGYDNLDVLDSTTSTPAYR"
     gene            2780..>3541
                     /locus_tag="VHE37_10965"
     CDS             2780..>3541
                     /locus_tag="VHE37_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HVT36101.1"
                     /translation="MNPNSDPRAAQAQQWACRELALPDAVFAPASADASFRRYFRLSD
                     GARSWIVMDAPPEREDSRPFVHVAGLMHEAGLRAPVIHAQDLSRGFLLLSDLGRQTYL
                     HVIDEQNADDLYGGAIEALIRWQRATRVGELPPYDEALLRRELALFPDWYVARHLERA
                     LTPAQRALLRASEDALVARALAQPRVYVHRDYMPRNLMISSVNPGVLDFQDAVLGPIS
                     YDVASLFRDAFLSWPTPRVYGWVRQYWERARAAHLP"
BASE COUNT          533 a         1129 c         1294 g          585 t
ORIGIN      
        1 cgcacctgct cgcgcaccgc caggaaatcc atgccctgct tcttcagctg ctcggaaaat
       61 tcggccagcg tcaccttgtt ctgctgcgcg atgttggtca tcacgtcgtt gagttcgcgg
      121 tcgtcgacgc ggatgccggc ctgctcggcg cgctgcgtct gcagcttgac cagcaccagg
      181 cggtccagca cctggccgcg caacacgctg tccggcggcg cctgcaggtt gcgctcggcg
      241 atggtcttgt gcgcgctgtc cagggcctgg tccagctcgc tctgcagcac cacgccgtcg
      301 ttgaccacca cgatgatgcg gtccagcgcc tcgggggcgg ccagcgccgc ggacgacagc
      361 aggacggtga cggcggcagc aaggccggtg cggaagatgt gcagcatgtg gtctgacgct
      421 cgatggaaaa ggcagcgggg cggcattatc gccgatggcg gggcgcccgt cagaccgcaa
      481 aaaactctac gggtttcagc ggtaggcggg ggtagaggtg gtgctgtcga gcacgtcgag
      541 gttgtcgtag ccggtgccga tgcgcgacag gcctttcagg ctcagctgca ggtagacgcc
      601 gctgttgaat tcaccgcgcg tggtcgagat gtagcgccgg taggaggtcg acagcgccca
      661 gcagcaggtg gagtattcga cgccggccag ggtgtcgagc ggggtgtggt cggcaatcga
      721 atagcgcacg cgcgcagtac cgcgccaggc gctgctcagc ggcgccgagc ccgcgatgtc
      781 ggcctgctcg aggatgttct ggcggtagcg gtaggccaca tcgaagcggc gcccgctgtc
      841 gcggtagtgc atggccacgc tggcgcggtt gaagcgatgg tcgcgcggcg accactgcga
      901 ggaaaatccc gcgatccaca ggtgcgacag gtggtagtcc aggctggcga tgaaatcagt
      961 ggcgccctgg ctgggcggcg gttgcgtcgg cgacggcagc tgcacctgcg gctgggtgaa
     1021 gcggtacagc tggccgatgg acgccgccag cttctcctcg ccgctgtacg ggttgagcag
     1081 gcgcgaggtg gcagccaccg ccagctggtt ggcatcggag atgcggtctt cgccggagaa
     1141 gcggttgcgc gcgaacaact gggtgaaatc gaaatccggg tcgccgctgt cgaacaccgg
     1201 cagctggtcc tggtcgcgga acggcgtgta gagatagaac acctggggct ccagcgtctg
     1261 cagcgagccg gcggcggtaa agcgctcgaa gcgcaggccg ctgccgatcg agaactgcgg
     1321 cagcgtgcgc gtgggactgt cgggctggcc cagggccgtg gcgccgagca ggtagtgggt
     1381 gtagtccagg ttcagctcgc tgttgaggaa ccaggtctgc tgctcgatct cggtcagcag
     1441 gtagggctgc agggtgagcc gctggccctg caccgagtcg gcgcgcagga agttggaata
     1501 ttcgctggac agtccggcgc gggtgtcgag caggctgttc ttggtgcgca cgcggaaatc
     1561 gatctgcggc aggcgccggt acggctggtc ggacagcgcc acggtctggt cgaccacctg
     1621 gtagtcctgc gccagcagag tcagggtgta ggccgagggc gcctggtagg tgagcagcag
     1681 gctgcggtcc agtgcggtca gcgcggtggc gtcgaccgcg ccgcccaggt cctcgaagta
     1741 ctgctggtcc gacacgtcgt agtagtgcgc ctccagcgac aggtgcgagg ccagcagtcc
     1801 tttgtggtcg aactgcaggt agctgcgctg ctcgccggtc tgcgcatcgt gcgccaggta
     1861 gtcgaagtgc aggtggcctt cgctctccgg ctccaggtag cgcgtgttgc tgcccagcag
     1921 cagcccgcgc ttggacatca cgcgcggcgt cagcgtggcg tcgtagttgg gccccaggtt
     1981 gaggtacagc ggccagcgga agtcgaagcc ggtgcgggtc gattcgccca cggtggggaa
     2041 cagcagcccg gtgcggcgct ggttgtcgat gggaaactgg aaccatggcg cgtacaggat
     2101 gggcacgccc aggaattcca gcttgacgtt gtgcgcggtg ccgacgccgg catgctggtc
     2161 cagcttgatg tcccgccctt ccaggtacca gccgcgcgag ccgggcgcgc aggtggtgta
     2221 gcgcaccgcc tggaactcgg cggtgccgtt cctgcccacg gtgagcacac cggcatcgcc
     2281 gcgcgcggca cgctgcggca gcgtgaaatc ggtgtcggag aacacgccgg tctcgctgtc
     2341 caggtcgaaa tccagacgct ggctcctgac gatgatgttc tggttgcgga acaacgactg
     2401 ctggcgcacg tgcacctggc gttccttgtc gtcgtaatcc atctgctgcg cggagaattc
     2461 cttgtcgccc tgctgcagcc tcaccgagcc ggccagcgac gacaggccgc cctgctcgag
     2521 cacgccctgg tcggcggtga cgtgcacgcg cgtgtcgccg gtgttcacga gcggcggcag
     2581 cgagtagtcg agctgcgggc agctggcttc ctccaccggc accgcggcgt gggcgcacac
     2641 cggcagcagc agcaggaaca ggcgggacag gcgcaagggc gtgcggacgc ttcgcagttt
     2701 ttggagcgcg agtatggtga aagacgtggc ggcggcgcaa ttgctaagct ctcgcgccgt
     2761 cgcaccgccg aacttttccg tgaatccgaa ctccgaccca cgcgccgcgc aggcgcagca
     2821 atgggcctgc cgcgagctgg ccctgcccga tgccgtgttc gcgccggcct cggccgacgc
     2881 cagcttccgc cgctatttcc gcctgagcga cggcgcgcgc agctggatcg tgatggacgc
     2941 gccacccgag cgcgaggaca gccggccgtt cgtgcatgtc gccgggctga tgcacgaggc
     3001 cgggctgcgc gcgccggtga tccacgcgca ggacctgtcg cgcggcttcc tgctgctgtc
     3061 cgacctcggc cgccagactt atctgcatgt catcgacgag cagaacgccg atgacctcta
     3121 cggcggtgcc atcgaggcgc tgatccgctg gcaacgcgcc acgcgcgtgg gcgagctgcc
     3181 gccctacgac gaggcgctgc tgcgccgcga actggcgctg ttccccgact ggtacgtggc
     3241 gcggcacctg gagcgcgcgc tgacgccggc gcagcgcgcg ctgctgcgcg caagcgagga
     3301 cgcgctggtg gcgcgcgcgc tggcgcagcc gcgcgtgtac gtgcaccgcg actacatgcc
     3361 gcgcaacctg atgatctcgt cggtcaaccc tggcgtgctg gatttccagg acgcggtgct
     3421 cgggccgatc agctacgacg tcgcctcgct gttccgcgac gctttcctgt cctggccgac
     3481 gccgcgcgtg tacggctggg tgcgccagta ctgggaacgc gcacgcgccg cgcacctgcc
     3541 g
//
LOCUS       DASZZC010000516         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1196824,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000516 DASZZC010000000
VERSION     DASZZC010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196824"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VHE37_10970"
     CDS             complement(<1..498)
                     /locus_tag="VHE37_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT36102.1"
                     /translation="MTNPDNPKRRDLLKGALTLGAGSTLAACGRSAPLSAAQAADASG
                     SYSAEIVVVGAGLAGLAAARNLVAAGKDALVVEARDRVGGRTWNHAVASSTRGGIVEI
                     GGQWVGPTQDRVLAYIQELGLSTFKTYDEGNYIDYRNGRLAPYGHIFPDPFNLGLNRI
                     PPSDPA"
     gene            complement(657..2018)
                     /locus_tag="VHE37_10975"
     CDS             complement(657..2018)
                     /locus_tag="VHE37_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36103.1"
                     /translation="MRPTRWAPATGAADARGTLRVVGIQYKQDVRHVVSYDSFRTKMR
                     CLVEDHAVPLMQPGQAMLVVFNEDIGLMTVATGARGAGVRKQAATPLRTPAGDAEPVG
                     VAGALVELNALYAPQITAYQTMYGPIDPRKQLFVGATDTFVRAFSQTFSDIARDYGVY
                     VVASNNMPGYRASSAAPDKLLFGDPEISADEVYIAASAHVANSTFLWGPDDIHPDAPV
                     GAKNLLFKNEKVPLTDIEKNLIALDQGPSTGEAARANAAGYTVAGWHLGFATSLPAFA
                     WGYDYGNAPPAGDPCADVTVSYMRCMDALGVEVVVQAEANPGRWAANAANGWQPLEWM
                     NSAWRTVNDAHVQFTYNINPMMTGNLLDLPFDGQSAITHRGTGGSAHYIGNLEFIDGT
                     DLPAYQPYVGDHASFAALAPWVTPDTSRAALTATGSALAPGSGDALENDYLETAVWAD
                     LVK"
     gene            complement(2204..2680)
                     /locus_tag="VHE37_10980"
     CDS             complement(2204..2680)
                     /locus_tag="VHE37_10980"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HVT36104.1"
                     /translation="MGVYDFSAKNIDGSEQKLDQYKGKVLLVVNVASKCGFTPQYKGL
                     EEIWRKHKDQGLVILGFPCDQFGHQEPGDENEIKNFCSLSYDVTFPLFAKIEVNGANA
                     HPLYQYLKSEAPGLLGSEGIKWNFTKFLIDKSGKVVKRYGSVDKPESIEKDIEKLL"
     gene            complement(2684..>3017)
                     /locus_tag="VHE37_10985"
     CDS             complement(2684..>3017)
                     /locus_tag="VHE37_10985"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVT36105.1"
                     /translation="QRLQQAEIWAAAYGSGGVFFARLLPVLRHLIGLPMGIVRMDFPR
                     YCLYTVLGSALWSAVLCWIGVQAGRDASLMQGDLHRITLWAIGGVAVLGTLYYLAVHR
                     TVAKRQGE"
BASE COUNT          484 a          965 c         1034 g          534 t
ORIGIN      
        1 ggccggatcg ctcggtggaa tgcggttcag gccgaggttg aatggatcgg gaaaaatgtg
       61 cccgtatggc gccagcctgc cgttgcggta gtcgatgtag ttgccctcgt cgtaggtctt
      121 gaacgtcgac agccccagct cctggatgta ggcgagcacg cggtcctgcg tgggcccgac
      181 ccactggccg ccgatctcga cgatgccgcc gcgcgtgctg ctggccaccg cgtggttcca
      241 ggtgcgcccg ccgacgcggt cgcgcgcctc gaccaccagc gcgtccttgc cggcggcgac
      301 caggttgcgg gcggcggcga ggccggcgag cccggcgccg accaccacaa tctctgcgct
      361 gtagctgccg ctggcatcgg cggcctgcgc cgccgacagc ggcgcgctgc ggccgcaggc
      421 ggccagcgtg ctgccggcgc cgagggtgag cgcgcccttg agcaggtcgc ggcgcttggg
      481 gttgtcggga ttggtcatgg gatgcgtagg aatcgttgaa tccgattagt gacgctgacg
      541 gcttcggtca gcaaccgtgg ttgtcgatgc gcataaggcg taatccgaga cggcgcaatg
      601 gttcatcgcg ttgccgtgct tgcgatggcg tcgccgcgta gcggcgggaa gatttctcat
      661 ttcaccagat ccgcccaaac cgccgtttcc aggtaatcat tctccagcgc atcgccgctg
      721 cccggcgcca gcgcgctgcc ggtcgcggtc aacgccgcgc gtgatgtgtc cggcgtgacc
      781 catggcgcca gcgcggcgaa gctggcgtga tcaccgacat agggttggta agccggcagg
      841 tcggtgccgt cgatgaattc caggttgccg atgtaatgcg cgctgccgcc ggtgccgcga
      901 tgcgtgatcg cgctttgccc atcgaacggc aggtcgagca ggttgccggt catcatcggg
      961 ttgatgttgt aggtgaattg cacgtgcgca tcgttcaccg tgcgccacgc ggaattcatc
     1021 cactccagag gctgccagcc gttggcggca ttggcggccc agcggccggg attggcctcg
     1081 gcctgcacca ccacctcgac gccaagggca tccatgcacc gcatgtagct gaccgtgaca
     1141 tcggcgcagg gatcgccggc tggcggtgca ttgccgtagt cgtaacccca ggcaaaggcg
     1201 ggcagggaag tggcgaagcc gaggtgccag ccggccacgg tgtaaccggc cgcgttggca
     1261 cgcgcggctt cgccggtgct ggggccttga tcgagcgcaa taagattctt ctcgatgtcg
     1321 gtcagcggca ccttctcgtt tttgaacagc agattttttg cgcccaccgg tgcatcgggg
     1381 tggatgtcat ccggccccca caggaaggtg ctgttggcga catgcgcgct ggcggctatg
     1441 taaacctcgt cggcagaaat ttccgggtcg ccgaacagca gcttgtcggg ggcggcgctc
     1501 gacgcgcggt agccgggcat gttgttgctg gcgacgacat acacgccgta gtcgcgcgcg
     1561 atatcggaaa aggtctgcga gaacgcgcgc acgaaggtgt cggtggcgcc gacgaacagc
     1621 tgcttgcgcg gatcgatggg cccgtacatg gtctggtagg cggtgatctg cggcgcgtac
     1681 aacgcattga gttcgaccag cgcgccggcg acgccgacgg gttcggcatc acccgcgggt
     1741 gtgcgcagcg gtgtcgcggc ctgcttgcgc acccccgcgc cgcgcgcgcc ggtggccacg
     1801 gtcatcaggc cgatgtcctc gttgaacacc accagcatgg cctggccggg ctgcatcagg
     1861 ggaacggcgt gatcctcgac caggcagcgc atcttggtgc ggaaactgtc gtagctgacg
     1921 acatgccgaa catcctgctt gtactggatg ccgaccacgc gcagcgtgcc gcgcgcatcc
     1981 gccgcgcccg ttgcgggcgc ccagcgcgtg ggacgcaatg cgagacaggt ggcagcggaa
     2041 ggacgctcgg ccgggagacg tggcccggat gcggggctgc tcgcccgcag aggcccggaa
     2101 gggttgcacg ccggcagcgc catcagcgca gcgagaaaaa tccccccggc gcttgcgcgc
     2161 cgcccccctt ttgccaagag ggccagcgcg cgctggccag ggatcagagc agcttctcga
     2221 tgtccttctc gatggactcg ggcttgtcga cggagccgta gcgcttgacc accttgccgc
     2281 tcttgtcgat gaggaacttg gtgaagttcc acttgatgcc ttcgctgccc agcaagcccg
     2341 gcgcctcgct cttgaggtac tggtacagcg ggtgcgcatt ggcgccgttg acctcgatct
     2401 tggcgaacag cgggaaggtc acgtcatacg agagcgagca gaaattcttg atctcgttct
     2461 cgtcgcccgg ttcctggtgg ccgaactggt cgcacggaaa gccgaggatc accaggccct
     2521 ggtccttgtg cttgcgccag atttcctcca ggcccttgta ctgcggagtg aagccgcact
     2581 tgctggccac gttgaccacg agcagcacct tgcccttgta ctggtcgagc ttctgttcgc
     2641 tgccgtcgat gttcttcgcg gaaaaatcgt agacgcccat gtgtcattcc ccttgtcgtt
     2701 tagccacggt acggtgcacc gccaggtaat acagcgtgcc gagcacggcc acgccgccga
     2761 tggcccatag ggtaatccgg tgcaggtcgc cctgcatcag cgaggcgtcc cggccggcct
     2821 gcacgccgat ccagcagagc acagcgctcc acagcgcgga gccgagcacc gtgtacaggc
     2881 agtagcgcgg gaaatccatg cgcacgatgc ccatcggcag gccgatcagg tggcgcagca
     2941 ccggcagcag gcgtgcaaag aacacgccgc cggagccata ggcggcggcc cagatttccg
     3001 cctgctgcag gcgctgc
//
LOCUS       DASZZC010000517         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_868083,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000517 DASZZC010000000
VERSION     DASZZC010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868083"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..953)
                     /gene="fabR"
                     /locus_tag="VHE37_10990"
     CDS             complement(264..953)
                     /gene="fabR"
                     /locus_tag="VHE37_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HVT36106.1"
                     /translation="MHRQVAKAAEAAEDAADSRGARKQRTREALLEAALRLMSEGRGL
                     TSLGLREITREAGVVPTSFYRHFRDIDELGLALVEEGGLTLRRLLREARKTGVPLADV
                     LRRSVMIYREYLEQNRLHFMFIAGERHGGSRAIRDAIRSEVRHFTSEMAADMRHLGFR
                     PELPMETLQLIAGLVVNTMLNAATDILDLPPGRSRAEDEMMENFIRQLRLIFLGALQW
                     QEKSDLPRRKK"
     gene            1087..>1609
                     /locus_tag="VHE37_10995"
     CDS             1087..>1609
                     /locus_tag="VHE37_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVT36107.1"
                     /translation="MSQPAVSKLSSGLQAALASAWLRPLNDATALDEVLSWIDPALTL
                     ATIKARVGAIIDETADTKTFVLQPNRHWRGHAAGQHVQVQLDIRGVRTQRTYTISSAP
                     GGDGIAITVKRQGRFSTHLHERVRVGDVLTLSAAAGNFVLPDVLPEKILMISGGSGIT
                     PVMAMLRELAARRY"
BASE COUNT          271 a          535 c          519 g          284 t
ORIGIN      
        1 tggcagctgc gcgcggccgc ggccatcccg ctgctgcggc gcctgccggc gcggctgatc
       61 ggcatgggct tccggccgga gcacgtgcgc agctagtgtc gtgaatctta agttcgttga
      121 aaaaaaataa tcgtcgtccc cgcgaaggcg gggacccagt tttggttccc ttcctcagca
      181 aaaataaaaa ctggattccc accttcgcgg gaatgacggg gagatttgtt caacgctttt
      241 gcaactcacc acactagagc gggctatttt ttccgccgcg gcagatccga tttttcctgc
      301 cactgcagcg cgccgaggaa gatcaggcgc agctggcgga tgaagttctc catcatctcg
      361 tcctcggcgc gcgagcggcc cggcggcagg tcgaggatgt cggtggccgc gttgagcatg
      421 gtgttgacca ccagcccggc gatcagctgc agcgtttcca tcggcagctc cgggcggaag
      481 cccaggtggc gcatgtcggc cgccatctcg ctggtgaagt ggcgcacctc gctgcggatg
      541 gcatcgcgga tcgcgcgcga gccgccatgg cgctcgccgg cgatgaacat gaagtgcagc
      601 cggttctgct ccaggtattc gcggtagatc atcaccgagc ggcgcagcac gtcggccagc
      661 ggcacgccgg tcttgcgcgc ctcgcgcagc aggcggcgca gggtcagccc gccttcctcg
      721 accagcgcca gacccagttc gtcgatgtcg cggaaatggc ggtagaacga ggtgggcacc
      781 acgcccgcct cgcgcgtgat ctcgcgcagg cccaggctgg tcagcccgcg gccttcgctc
      841 atcagccgga gggctgcttc cagcagggcc tcgcgcgtgc gctgcttgcg cgcgccgcgg
      901 ctgtcggccg cgtcttccgc cgcctcggct gcctttgcga cctgtctgtg cacggttgta
      961 gaaatcgatt gtgtacgctt gtactggtga aattatcagt caaaacaagc acaaaaagcc
     1021 aggactattg accggccacg tcattttcag tgaacatctg tacaccgata gtcccggagc
     1081 cgcagcatgt cacagcccgc agtatccaag ttgtcgtccg gcttgcaagc cgcactggcc
     1141 tcggcctggc tgcgtccgct caacgacgcc accgcgctcg acgaggtgtt gtcgtggatc
     1201 gacccggcgc tgaccctggc caccatcaag gcgcgcgtgg gcgcgatcat cgatgagacc
     1261 gccgatacga agaccttcgt gctgcagccc aaccgccact ggcgcggcca cgccgccggc
     1321 cagcacgtgc aggtgcaact ggacatccgc ggcgtgcgca cgcagcgcac gtatacgatt
     1381 tcatcggcgc cgggcggcga cggcatcgcc atcacggtca agcgccaggg ccgcttctcc
     1441 acccacctgc atgaacgggt gcgcgtcggc gacgtgctga cgctgtcggc ggcagcgggc
     1501 aacttcgtcc tgccggacgt gctgccggaa aaaatcctga tgatctcagg cggcagcggc
     1561 atcacgccgg tgatggccat gctgcgcgag ctggcggcgc gccgctacg
//
LOCUS       DASZZC010000518         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_948218,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000518 DASZZC010000000
VERSION     DASZZC010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948218"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VHE37_11000"
     CDS             complement(<1..375)
                     /locus_tag="VHE37_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36108.1"
                     /translation="MTSFHPAARLLAALVPLAWSMPAPALPTLAVSAPADAALMHEGQ
                     TRVAHANDALAAGDAVTAAGSGGTTLQLDRYGALELGPAATLSVLRLPFSSYASDLAT
                     ALKLDGGYLRIVWKYPALSAPWP"
     gene            461..994
                     /locus_tag="VHE37_11005"
     CDS             461..994
                     /locus_tag="VHE37_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36109.1"
                     /translation="MSTTRWIAATTWLFCAVAATGCQSPDARDAAPPACTADDMKDAP
                     LLVLPDTHAPQNLPTDKSAPYQYAVALPVHRDLLQAGRWNPLAGGWSALTLRVTSQNA
                     SSLSARFNPLSLPAGAQLWLCAADGRTRQGPYGDSGGELLTAVVSGNDALIEVLVPIA
                     AQPAVRINLSEMHAGFR"
     gene            complement(960..>2384)
                     /locus_tag="VHE37_11010"
     CDS             complement(960..>2384)
                     /locus_tag="VHE37_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36110.1"
                     /translation="YFHRALEATLPGMAFYLVGDNGSEEDAETVPLVSKCGGCYQQAQ
                     ATGEGYAIAVAAAVPAAVPLAYGPISAARSEFFVPIENNLFKAAAKLGIFGDRPTYIG
                     GVAAGSLGSDLQSVVTVAAVGPDLQLIGIPGEGFPGLIVGNPWGIEDAPCTTNVPGEP
                     ARDNPPVPAWHARAPFRLESGLTGDFMGYMLPAWAFVSETGTYLSTCHTDSDDKDSKG
                     HQHKLEDEGVGPTASNSVASNLAALLDATPDATASIVTGRYLYADGSSARRPLVTQNG
                     GTVQAVGIWLTDSGSTDLAPGKGSIVALSGIGAFGTQAVSANGRYMDYDGNEQAAPDI
                     TTRGMLVRDANGTVLKRYYLDLYPALTVANAGLANAGGGTGSTTGATTGATTGSTTGS
                     STGSATGSTTGSTTGSTTGATSGSSTGADTGSSTGGATGATPASTFGSGGGGAMPSSL
                     LVLALLAVARRRTFSGSRRASPTG"
BASE COUNT          352 a          825 c          823 g          384 t
ORIGIN      
        1 cggccacggc gccgacagcg ccgggtactt ccacacgatg cgcaggtatc cgccatcgag
       61 cttgagcgcc gtggccaggt cgctggcgta actcgagaac ggcaggcgca gcaccgacag
      121 cgttgcggcc ggacccagct ccagcgcgcc gtagcggtcg agctgcaagg tggttccgcc
      181 cgagccggcc gcagtcaccg catcgccggc cgccagggca tcgttggcgt gtgccacgcg
      241 cgtctgccct tcgtgcatca gggcggcatc ggcaggcgcg gagacggcaa gcgtgggcag
      301 cgccggtgcc ggcatgctcc aggcgagcgg tacgagtgcg gccagcaggc gtgccgcggg
      361 gtgaaaggag gtcacaagcg ttcctcgtca ggacgtgatc ggatcgcggc ggcgcgcggg
      421 ctacactcat tgccccctcc ttattttttt gggcaacgac atgtcgacca cacgctggat
      481 cgccgcgaca acctggttat tctgcgccgt tgcggcaacc ggctgccagt cgcctgatgc
      541 gcgcgatgcc gcgccgccgg cctgcacagc ggacgacatg aaggacgcgc cgctgctggt
      601 actgcccgat acccacgcgc cgcagaacct gccgaccgac aagagcgcgc cgtaccaata
      661 tgcggttgcc ctgcccgtcc accgcgacct gctgcaggcc gggcgctgga atcccctggc
      721 gggcggctgg tcggcgctga ccttgcgcgt gacctcgcag aacgcaagct cgctgtctgc
      781 ccgcttcaat ccgttgtcgc tgcccgctgg cgctcagcta tggctgtgcg ccgccgacgg
      841 ccgcacgcgt cagggccctt acggcgattc cggcggcgaa ctgctcaccg ccgtggtcag
      901 cggcaatgac gcgttgatcg aagtgctggt gccgatcgcc gcccagccgg cggtgcgcat
      961 caacctgtcg gagatgcacg ccggcttccg ctgaaggtcc ggcgccgcgc aaccgcgagc
     1021 agggccagta ccagcagcga cgatggcatg gcgccgcctc cgccactgcc gaaggtcgac
     1081 gcgggggtgg cgccggtggc gccgccggtg ctgctgccgg tgtccgcccc ggtggacgaa
     1141 ccgctggtgg caccggtggt cgatcccgtc gttgaacccg tcgtcgagcc tgtggccgaa
     1201 ccggtggacg accccgtggt cgatccggtg gtggcgccgg tagtcgctcc ggtcgtggaa
     1261 ccggtgccgc cgccggcgtt ggccaggccg gcattggcca cggtgagcgc cgggtagagg
     1321 tccaggtaat agcgcttgag cacggtgccg ttcgcgtccc tcaccagcat gccgcgggtg
     1381 gtgatgtcgg gcgccgcctg ttcgttgccg tcgtagtcca tgtagcggcc gttcgcgctc
     1441 accgcctgcg tgccgaaagc gccgatgccg gagagcgcaa cgatgctgcc cttgccgggt
     1501 gccaggtcgg tgctgccgct gtccgtcagc cagatgccca cggcctgcac cgtaccgccg
     1561 ttctgcgtca ccagcggacg ccgcgcgctg ctgccgtcgg catatagata gcggccggtg
     1621 acgatgctgg ccgtcgcgtc gggcgttgca tcgagcagcg cggccagatt gcttgctacg
     1681 gaattcgaag cagtgggacc gacgccctca tcttcgagct tgtgttgatg tcccttgctg
     1741 tccttgtcgt cgctgtcggt gtgacaggtg ctgagatagg tgccggtctc ggacacgaaa
     1801 gcccaggccg gcagcatgta gcccatgaaa tcgccggtca gcccggactc cagccggaac
     1861 ggcgcgcgcg cgtgccaggc cggtaccggc ggattgtcac gcgccggttc ccccggaacg
     1921 ttggtggtgc acggcgcatc ctcgatgccc cagggattgc cgacgatcaa cccgggaaag
     1981 ccttcaccgg gaatgccgat cagctgcagg tccggcccca ccgcggccac cgtcaccacg
     2041 ctttgcagat cgctgcccag cgagccggcc gccacgccgc cgatgtaggt ggggcgatcg
     2101 ccgaagatgc ccagcttggc cgcagccttg aacaggttgt tctcgatcgg cacgaagaat
     2161 tcgctgcgcg cggcgctgat cgggccgtac gccaacggca ccgccgctgg tacggcggca
     2221 gcaacggcga tggcgtagcc ctcaccggtg gcctgcgcct gctggtagca gccgccacat
     2281 ttggatacca gcggcacggt ctcggcatct tcctccgagc cgttgtcacc gaccaggtag
     2341 aaggccatgc ccggcagcgt cgcctccagc gctcggtgaa aata
//
LOCUS       DASZZC010000519         7826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_620110,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000519 DASZZC010000000
VERSION     DASZZC010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620110"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VHE37_11015"
     CDS             <1..598
                     /locus_tag="VHE37_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HVT36111.1"
                     /translation="NLRRFDKGGDAFYDQISALHKSVRGSDPDAALYWFARMIDGGAD
                     PRYVARRIARMAFEDIGLADPRALEMCLAAWEAYERIGSPEGDLALAQAVVYLAVAPK
                     SNAVYEAYGAARAAVEQHGSLEVPMHIRNAPTRLMKELGYGKGYRYDHVEPGAHAEGQ
                     EFLPEALRSTRFYHPTERGFESRIAEKLQRLRGGKEES"
     gene            601..972
                     /gene="crcB"
                     /locus_tag="VHE37_11020"
     CDS             601..972
                     /gene="crcB"
                     /locus_tag="VHE37_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HVT36112.1"
                     /translation="MNLPLWLAVALGGAAGSLLRWQVSVWLRSAWPAFPWGTLLVNVS
                     GGFLMGFVAAWCVARPVPDWMRIGMMTGIMGGYTTFSAFSLDTLELWRQGAAPALMNI
                     AANLLLSLGACALGLWAARAI"
     gene            1030..2304
                     /gene="serS"
                     /locus_tag="VHE37_11025"
     CDS             1030..2304
                     /gene="serS"
                     /locus_tag="VHE37_11025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVT36113.1"
                     /translation="MLDPKRLRSDAAGVARLLARRGYTLDLEKFAQLESRRKALQSET
                     ESLQAERNAGSKRIGQAKSRNEDVAPILEAMEQIKGKLAANEQALTALQAEFEDFALR
                     VPNVPHASVPDGADESHNVEVRRWGAPRPTGPATDHAELGEALGMMDFAAAAKLTGAR
                     FTVLKGQLARMHRALIQFMLDVHTQEHGYEELYVPYIVNAASLRGTGQLPKFGEDLFA
                     LKGEVDWRLIPTAEVPVTNLLRDEIVSAESLPRKWVAHTPCFRAEAGAAGKDTRGMIR
                     QHQFEKVELVQATLPEHSHEAHEQLTGHAEAILKKLELPYRVVNLCAGDLGAGSAKTY
                     DLEVWLPSQQKYREISSCSNFEDYQARRMMARYRSAAAGKPELLHTLNGSGLAVGRTL
                     VAIMENYQQEGGAIAVPAALQAYLPWKTISGK"
     gene            complement(2343..2465)
                     /locus_tag="VHE37_11030"
     CDS             complement(2343..2465)
                     /locus_tag="VHE37_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36114.1"
                     /translation="MTTSGSVTTILNVTLVVNGESVTVDISFDSDSNQVTITRA"
     gene            2512..2730
                     /locus_tag="VHE37_11035"
     CDS             2512..2730
                     /locus_tag="VHE37_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36115.1"
                     /translation="MACGLTLMTVWPVPLDAACERALAVLELAMMLPAGADPGPGEVA
                     EPAYARLEANIAEMTSAVFMCPPLNGVG"
     gene            complement(2738..3400)
                     /locus_tag="VHE37_11040"
     CDS             complement(2738..3400)
                     /locus_tag="VHE37_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36116.1"
                     /translation="MAFGAGWGDLGVGIGGATQPGSGGQHYDGSASIVGGIGNPDKYV
                     GLEAGLNIISLRDSGAGDSFAHDGNMAFKFHSNLPGEVSVGVGVENVVRWGKPKTGDP
                     AKGIPPTRSSVYGVGTRIFDLNPGGSNKLPLAVNLGVGNHRFTSSGKGSVGAFGGVAL
                     LPLPSLSLIADWTGPELNAGVSWAPLPRWPLTVSLGAANVTSRRAGHPEFAGGIGYGF
                     HY"
     gene            3994..4767
                     /locus_tag="VHE37_11045"
     CDS             3994..4767
                     /locus_tag="VHE37_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36117.1"
                     /translation="MMRGMANRGMMLAGMLFVAMPAFSQSVPVANTANMAAAMANFST
                     SSAAPALQVPGGGGSGWGSIGVGVYGQTARGTPARADGSLGLNFGLFDPAKYFGLDLS
                     VSFTSLTGANGSRAFDAGTLGYKLHTNLPGLVSFGIGATSVQRWGVAKANKADEYASL
                     SKYFPMGAYALSATVGVGDGGFTTDGKGVAGYGSLAFYLTPRISFIGEFTGRFTNFAV
                     SWAPLRLHPLSVTLGAVNLTQWHGLDTQVAASVGYGFGF"
     gene            4800..5153
                     /locus_tag="VHE37_11050"
     CDS             4800..5153
                     /locus_tag="VHE37_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36118.1"
                     /translation="MKGKAVVVAAALAMMSAAPVWAQSTGNPGGSTGSTGSSGSTGGG
                     TNVDSAAAFAAGNLPAGALAGSVGAQVASILSGGGSPASMKAALAALLASVQAGGGSG
                     AGPVASAISNLIAAL"
     gene            complement(5206..5700)
                     /locus_tag="VHE37_11055"
     CDS             complement(5206..5700)
                     /locus_tag="VHE37_11055"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HVT36119.1"
                     /translation="MTILTRDGNIVANDYALLADDAGATGAAAIVTWQRWMKERESLR
                     AAVARIGVRLPNTLDVIPAFPDLADRPLLELEFPAFADGRAYSQARLLRQRCKYQGEI
                     RAVGNAVVRDQLFGMARCGFDSFELRADQNASVCVSALRDFSLAYQPAADGIAPVGRL
                     RWGG"
     gene            complement(5697..7370)
                     /locus_tag="VHE37_11060"
     CDS             complement(5697..7370)
                     /locus_tag="VHE37_11060"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HVT36120.1"
                     /translation="MYQYDQYDQKLVDERVAQFRDQTRRFLDGQIPEDEFRSLRLRNG
                     LYIQRFAPMLRISIPYGLLSSKQLRMLAHIARKYDKGYGHFTTRQNIQFNWPKLPEVP
                     DILADLATVQMHAIQTSGNCIRNITADHLAGAAADELLDPRPYCEITRQWATFHPEFN
                     WLPRKFKIAFSSSTTDRAATQVHDIGVHIVRNAAGELGYTIYAGGGLGRMPMIAPKIR
                     DFLPELELLSYLEAILRVYNRLGRRDNIHRARIKILVKDLGAAKFTEMVEAEWAQLKG
                     GELRLAPDAVARIARQFTLPAYEPGAAADTSFARRQQESSAFAAWAKRNVKLHRQAGY
                     RAVFISLKAPDVAPGDVTAGQMDAIAALADRYSFGELRATHDQNLVFADVRQQDLYEL
                     WQRLSELNLATPNIGLLTDLICCPGLDFCSLANAGSIGVAEDIFDRFDNYDHLHDIGE
                     IKIKMSGCMNGCGHHSVGHIGILGVDKKGEEWYQVTLGGSAENDASLGDRTGPSISRE
                     EIADAVERIIQVYLEQRDSAEETFLQTYRRIGMAPFKQRIYAEAEAVAA"
     gene            complement(7477..>7826)
                     /locus_tag="VHE37_11065"
     CDS             complement(7477..>7826)
                     /locus_tag="VHE37_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVT36121.1"
                     /translation="HGLKPDVVLTAVDADVIKTYVRNGLGIGIIANMAWDERTDRDLV
                     RLPADHLFEPSTTHIGFRRGMFLRGYMYDFIHAFAPHVTRELIDEMAAIADHEQRLKR
                     TEELVSSLPQQKH"
BASE COUNT         1341 a         2382 c         2651 g         1452 t
ORIGIN      
        1 gaacctgcgc cgcttcgaca agggcggcga cgccttctac gaccagatca gcgcgctgca
       61 caagtccgtg cgcggatcgg atcccgacgc cgccctttac tggttcgcgc gcatgatcga
      121 cggcggcgcc gacccgcgct acgtcgcccg gcgcattgcg cgcatggcct tcgaagatat
      181 tggcctggcc gatccgcgcg cgctggagat gtgtcttgcc gcgtgggaag cctacgagcg
      241 catcggcagc cccgaaggcg acctggcgct ggcccaagcg gtggtctatc tggcggtcgc
      301 gcccaagagc aacgcggtgt acgaggccta tggcgcagcg cgcgccgcgg tggagcagca
      361 tggctcgctg gaagtgccga tgcacatccg caacgcgccg acgcggctga tgaaggagct
      421 gggttacggc aagggctacc gctacgatca cgtcgagccc ggcgcgcacg cggaaggaca
      481 ggagtttctg cccgaggccc tgcgcagcac gcgcttctat catccgaccg agcgcggctt
      541 cgagagccgg attgcggaaa agctgcagcg cctgcgcggc ggcaaggaag aaagctgagc
      601 atgaacctgc cactctggct cgcggttgca ttgggaggtg cggcgggttc gctgctgcgc
      661 tggcaggttt cggtgtggct gcgctcggca tggccggcat ttccgtgggg caccttgctg
      721 gtgaacgtgt ccggcggatt cctcatgggt ttcgtcgccg cctggtgcgt ggcgcggccg
      781 gtgccggact ggatgcgcat cggcatgatg accgggatca tgggcggcta caccacgttc
      841 tccgctttct cgctcgacac gctggagctc tggcgccagg gcgctgcgcc cgcgctgatg
      901 aacatcgcgg ccaatctcct gctcagcctg ggcgcctgcg cgctcggcct gtgggccgcc
      961 cgcgcgatct gaattaattt tccgcgtcgc tatacttcgc gccctgtctt tcaagcggaa
     1021 attgcaccga tgctcgaccc caaacgcctg cgcagcgacg ctgccggcgt tgcgcgcctg
     1081 cttgcgcgac gcggctacac gctcgatctg gaaaaattcg cgcagctgga atcgcgccgc
     1141 aaggcgctgc agagcgagac tgaatcgctt caggccgaac gcaacgccgg ttccaagcgc
     1201 atcggccagg ccaagagccg caatgaagac gtggcgccga tcctcgaggc gatggagcag
     1261 atcaagggca agctcgcggc caacgagcag gcgctgaccg cgttgcaggc ggagttcgag
     1321 gactttgcgc tgcgcgtgcc caacgtgccg cacgcctcgg tgcccgatgg cgccgatgaa
     1381 tcgcacaacg tcgaagtgcg ccgctggggc gcgccgcgcc cgactggccc cgctaccgat
     1441 catgcggaac tgggcgaggc gctgggcatg atggattttg ctgccgcggc caagctcacc
     1501 ggcgcgcgct tcaccgtgct caaggggcag ctggcgcgca tgcaccgcgc gctcatccag
     1561 ttcatgctcg atgtgcacac gcaggagcat ggctacgaag agctctacgt gccgtacatc
     1621 gtcaacgccg ccagcctgcg tggcaccggc cagttgccga agttcggcga ggacctgttc
     1681 gcactcaagg gcgaggtcga ctggcgcctg atcccgacgg cggaagtgcc ggtgaccaac
     1741 ctgctgcgcg acgagatcgt ttccgccgaa tcgctgccgc gcaaatgggt ggcgcacacg
     1801 ccgtgtttcc gcgcggaagc cggtgccgcg ggcaaggaca cgcgcggcat gatccgccag
     1861 caccagttcg agaaggtgga actggtgcag gcgaccttgc ccgagcattc gcatgaagcc
     1921 catgaacagc tcaccgggca cgccgaggcc atcctgaaga agctcgagct tccgtatcgg
     1981 gtggtgaatc tttgcgccgg cgatctgggc gcgggttcgg ccaagaccta cgatctcgaa
     2041 gtttggctgc cgtcgcaaca gaaataccgc gagatttcct cgtgctccaa tttcgaggat
     2101 taccaggcgc gccgcatgat ggcgcgctac cgctcagcag cggcaggaaa acccgagctg
     2161 ttgcacacgc tcaatggatc ggggcttgca gtcgggcgca cgctggtggc catcatggag
     2221 aactaccagc aggagggtgg cgccattgca gtgcccgccg cgctgcaggc ctacctgccc
     2281 tggaagacca tctccggcaa ataaaaaaag cgcctggaag ggcgcttttt ttgtggagca
     2341 gatcaggctc gcgtgatggt cacctggttc gagtcgctgt cgaaggagat gtcgacggtc
     2401 accgattctc cattgacgac aagggtgacg ttgaggatgg tggtcacgct gccggacgtg
     2461 gtcaccgagg ctccggcggg cgggttctgt gctaggacgt tcagcgacga aatggcctgc
     2521 ggactgacgc tgatgaccgt gtggcccgtt ccgctggatg cagcctgtga aagggctttg
     2581 gccgtgctcg aattggcgat gatgctgccg gctggagccg atcccggtcc cggcgaagtt
     2641 gctgaacctg cgtacgccag gctggaagcg aatattgctg aaatgaccag tgcggttttc
     2701 atgtgtcctc cgctaaacgg ggtaggttga cgctgcttca gtaatggaag ccgtagccaa
     2761 tgccgccggc aaattcggga tgacccgcgc ggcggctggt gacgttggct gcgcccaggg
     2821 aaaccgtcaa gggccagcgc ggcagtggcg cccaggaaac accggcgttc aattccggcc
     2881 cggtccagtc ggcgatcagc gataggctcg gcagcggtaa cagggccacg ccgccgaagg
     2941 cgccaacgct acccttgccg gaactggtga agcgatggtt gccgacaccg agattgacgg
     3001 ccagcggcag cttgttcgag ccgcccggat tcagatcgaa aatcctggtg ccgacaccat
     3061 agacactgct gcgtgtgggg ggaatgccct tggccggatc gcccgtcttg ggtttgcccc
     3121 agcggaccac gttttccacg ccgacgccca cggagacttc gccgggcaga ttggagtgga
     3181 acttgaaggc catgttgccg tcatgcgcga agctgtcgcc agcgccgctg tcacgcaggc
     3241 tgatgatgtt gagtcccgct tccaggccga cgtacttgtc gggattgccg atgccgccga
     3301 cgatgctggc ggagccgtcg tagtgctggc ctcccgagcc tggttgggtg gcgccaccga
     3361 taccgacgcc gaggtcaccc cagccggcgc caaaagccac cggagatccg aaagcaatgc
     3421 ccggtgaagc cttggccggt ccctgtcctg cgaggagggc ttcgacctgt gcctggctga
     3481 ggcccagctg gtcggccgtg acgctctggg cgccggcctg atgcgtggcc acaagcgcga
     3541 aggagcaaag ccatattgca gaaagcctga cgccccctga actgtttgac ttcatccctg
     3601 agccctcttt cgttgtgaat attagattcg caagcctaac gtgcagaacc ggagccgacc
     3661 ttatttaacc acaatggctc cgggcgacgt atcggcgaat tcctcgccgt ttttcggaaa
     3721 atgcccgcgc ccatgcaccg gcaccggcag aatttttgca ggcaaaatac cggccctgcg
     3781 ttttgcaggt tgtgtcgctg cagggaagta ggctcgcggc tcgcttgaag aatcgggtga
     3841 tctacgtcac cgcaacgcgt taagtctgcg gtttatcatg caagaaattg ctgaaaccta
     3901 gcggttgcag gttgtgattg acctcacacg gcgctaaagt gagatggttg ggcttttaat
     3961 tcaaattaac cgactaggct gggagaagca gagatgatga gagggatggc gaacaggggc
     4021 atgatgctgg cagggatgct gttcgtggct atgccggcct tttcccagtc agtgcccgta
     4081 gccaacaccg ccaatatggc ggcggccatg gcgaattttt cgacgagttc ggcggcgcct
     4141 gccctgcagg tgccgggcgg cggcggttcc ggctggggat cgatcggtgt tggcgtttat
     4201 ggacagaccg cgcgcggcac gccggcgcga gccgacggtt ctctcggtct gaacttcggc
     4261 ctgttcgatc cggccaagta tttcggtctc gacctgtcgg tcagcttcac ctcgctgacg
     4321 ggcgccaacg gcagccgcgc ttttgacgcg ggcacgctgg gctacaagct gcacaccaac
     4381 ctgcctgggc tggtctcctt cggcatcggc gccacttcgg ttcagcgctg gggcgtggca
     4441 aaggccaaca aggcggatga gtatgcttcg ctgtcgaagt atttccccat gggtgcgtat
     4501 gccttgagcg caaccgtagg cgtaggtgat ggcggcttta ccaccgacgg aaagggtgtg
     4561 gcggggtacg gctcgctggc gttctacctg acgccgcgga tttcgttcat cggcgaattc
     4621 accggccgtt tcaccaactt cgccgtatca tgggcgccgc tgcgcttgca tccgctgagc
     4681 gtgacgctgg gagcagtcaa tctgacccag tggcatggtc tggatacgca ggttgcggcc
     4741 agcgtgggct atggtttcgg tttctaggac gtgcgcgaat ttgatgtagg agaagtgcaa
     4801 tgaagggtaa agcagtggtg gttgcagcgg ccttggcgat gatgtcggcg gcgccggtat
     4861 gggcgcagtc gaccggcaat cctggcggct cgaccggcag caccggcagt tccggttcga
     4921 ccggcggcgg caccaacgtg gacagcgcag cggcgtttgc ggcaggtaac ctgccggcgg
     4981 gtgctctggc gggttcggtg ggcgcgcagg tggcctcgat tctcagcggc ggtggttcgc
     5041 cggcctcgat gaaagcggca ctcgcggccc tgctggcatc ggtccaggcg ggtggcggct
     5101 ccggtgcggg ccctgtcgcg agcgcgatca gtaacctgat tgccgcgctt tgagtttctt
     5161 gcgcacggtg cgtgccgggt tccggcacgc accgccgcgc atggttcagc cgccccagcg
     5221 caagcgtccg accggcgcaa ttccatctgc agcaggctgg taggcgagcg agaaatcgcg
     5281 taaggccgac acgcagaccg acgcattctg gtctgcccgc agttcgaacg agtcgaagcc
     5341 gcatcgcgcc attccaaaaa gctggtcccg caccacggca ttgccgacgg cgcggatttc
     5401 accttgatac ttgcagcgct gccgcagcag acgtgcctgg ctgtacgccc ggccatcggc
     5461 gaaagctgga aattccagtt cgaggagcgg gcggtcggca agatccggga aagctgggat
     5521 cacatccagc gtgttgggca ggcgcacgcc gatcctggcg acggctgccc gcagcgactc
     5581 gcgttccttc atccaccgct gccaggtcac gatagctgct gcgccggtgg caccggcatc
     5641 gtccgccagc aaggcgtaat cgttcgcaac gatgttgccg tcccgcgtca atatcgtcat
     5701 gcagcgactg cctctgcttc ggcatagatg cgctgcttga acggcgccat gccgatgcgg
     5761 cgataggtct gcaggaaggt ttcttccgcg ctgtcgcgct gctccaggta gacctggatg
     5821 atgcgttcca ccgcatcggc gatttcctcg cgggaaatcg acgggccggt gcggtcaccc
     5881 aggctggcgt cattctcggc cgagccgccg agcgtcacct ggtaccactc ctcgcctttc
     5941 ttgtcgacgc cgaggatgcc gatgtggcca acgctgtgat ggccgcagcc gttcatgcac
     6001 cccgacatct tgatcttgat ctcgccgatg tcgtgcaggt gatcgtaatt gtcgaagcgg
     6061 tcgaagatgt cctcggccac gccgatcgag ccggcattgg ccagcgagca gaagtcgagc
     6121 cccgggcagc agatcaggtc ggtgagcagc ccgatgttgg gcgtcgccag attgagttcg
     6181 ctcagccgct gccacaactc gtacaggtct tgctgccgca cgtcggcgaa gaccaggttt
     6241 tgatcgtgcg tcgcgcgcag ttcgccgaac gagtagcgat cggcgagtgc agcgatcgcg
     6301 tccatctgtc cggcagtgac gtcgcccggc gccacgtccg gcgccttgag cgagatgaat
     6361 accgcgcgat atcccgcctg ccggtggagc ttgacgttgc gcttggccca ggccgcgaag
     6421 gcgctgcttt cctgctgcct gcgggcgaag ctggtgtcgg ctgccgcgcc gggctcgtag
     6481 gccggcagcg tgaactgccg ggcgatgcgg gccacggcgt cgggtgccag ccggagctcg
     6541 ccgcctttca gctgcgccca ctcggcctcg accatctcgg tgaacttggc tgcaccgaga
     6601 tccttcacca ggatcttgat gcgtgcacgg tggatgttgt cgcgccggcc caggcggttg
     6661 tacacgcgca ggatggcctc gagataggac agcaattcca gttcgggcag aaaatcgcgg
     6721 atcttgggcg cgatcatcgg catgcggccg aggccgccgc cggcatagat ggtgtagcca
     6781 agttcgccgg cggcgttgcg cacgatgtgc acgccgatgt cgtgcacctg cgtggcggca
     6841 cggtcggtgg ttgacgagga gaacgcaatc ttgaacttgc gcggcagcca gttgaactcg
     6901 ggatgaaacg tggcccactg gcgggtgatc tcgcagtacg gccgcggatc gagcagttcg
     6961 tcggccgccg cgccggccag gtgatcggcg gtgatgttgc ggatgcagtt gcccgaggtc
     7021 tggatggcat gcatctgcac ggtggccagg tcggccagga tgtccggcac ttccggcagc
     7081 ttgggccagt tgaactggat gttctgccgg gtggtgaagt gcccgtagcc cttgtcgtac
     7141 ttgcgcgcaa tatgcgccag catgcgcagc tgcttgctcg acagcaggcc atagggaatg
     7201 gagatgcgca gcatcggcgc gaagcgctgg atatacaggc cgttgcgcag gcgcagcgag
     7261 cggaactcgt cttccggaat ctgcccgtcc aggaagcggc gcgtctggtc gcggaactgc
     7321 gcgacacgct catcgacgag cttctggtcg tattggtcgt actggtacat gctgggccgg
     7381 aaaagctgct gcggtgaaat tttcggggcg caacgataac aggtcggggc cggcgccaga
     7441 agcggcggcg ggagctaact ttatagtgat atggaattag tgcttctgct ggggcagaga
     7501 tgacaccagt tcctcggtgc gcttcaaccg ctgctcgtgg tcggcgatcg cggccatctc
     7561 gtcgatcagc tcgcgcgtca cgtgcggggc gaaggcatgg atgaagtcat acatgtagcc
     7621 gcgcaggaac atgccgcgcc ggaagccgat atgcgtggtg ctgggctcga acaggtggtc
     7681 cgccggcagc cgcaccaggt cgcggtcggt gcgctcgtcc caggccatgt tggcgatgat
     7741 gccgatgccc agcccgttgc gcacgtaggt cttgatcacg tcggcatcga ccgcggtcag
     7801 caccacgtcg ggcttgagtc cgtggg
//
LOCUS       DASZZC010000520         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_103545,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000520 DASZZC010000000
VERSION     DASZZC010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103545"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VHE37_11070"
     CDS             <1..1425
                     /locus_tag="VHE37_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36122.1"
                     /translation="HQRSEMGQPEAFGASCRNGAHPPDPQHCTDGLGYADTDGDPYKG
                     DWRGDFPYNVDQLGETLIADWDLGAATLTSVTGYIDFKRNFHIDTDGSPADEFDFFQT
                     DTVKQTTQELRLSGGHGAAQWLAGVFWSRDDIHVHDDGRHEDAIPGEFSTIEALQGTR
                     SAAAFGNVDWKLSERWTLTSGLRYTHESRDYSGGTHWTVPVPGTIASTSTDSSIHDSN
                     WSWKLGASFAPTHTSLIYANASKGVKSGGYFSGVTTDSAQLQPYLPEQLVAYEIGAKT
                     QGAFALNASVFYYDYKNVQTFMRNASAPVQLIGNVPKASTMGLDAEAILRALDGLTLQ
                     TGVGLLHTRLGSFDGPDGSPVAAGNQLPNSPRITFNGLARYEFPLFSEDVLGALQADA
                     HYSASAFKEATDDPVIHQDPYWIFNARVGVLSASRVWEVAAWGRNLTNKLYVVQGLDV
                     GSLFFGNRNYNAPRTAGVEFSVKF"
     gene            1436..1744
                     /locus_tag="VHE37_11075"
     CDS             1436..1744
                     /locus_tag="VHE37_11075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HVT36123.1"
                     /translation="MLDIFFYLTGKSGKDSELRALLGEMTRVSRADDGCIHYTFHQQL
                     DDRRKWVLHEQWRDRAALDAHIASMKKHFGEPPPGARLPQRLHELHESSSYAFHEVLA
                     "
     gene            1741..2763
                     /locus_tag="VHE37_11080"
     CDS             1741..2763
                     /locus_tag="VHE37_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVT36124.1"
                     /translation="MNLRNRRWVLKTRPLGMPARSDFELREEPLPALADGQFLARNLY
                     LSCDPAQRSWLARDTYVPKIAIGETMRAGATAQVVESRHAQFNTGDIVSGMFGWQDYA
                     VSDGSGFVPVSKLPAGVPVPLAMSALGLTGLTAYYGMLEVAEVRAGQNVLVSGAAGAT
                     GSVAAQLAKMRGARVVGVAGGAAKCQYLLDQLRLDGAIDYRAGDVGAIIREMFPKGVD
                     IYFDNTGGEVLEQALLRLSTGGRIVLCGAISSYNDLDATPPLKNWSKLLVNRGRVQGI
                     LVTDFAPYFARAAGDLARWVAEGKIKNRVDIVEGLEQAPDALRRLFTGDNIGKQLVKL
                     AEPAAG"
     gene            complement(2869..>3315)
                     /locus_tag="VHE37_11085"
     CDS             complement(2869..>3315)
                     /locus_tag="VHE37_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36125.1"
                     /translation="QFKYGTHGSLPGPEPVGTFTDLGDLDASSNYTTDGVITLVLDTT
                     AIGLKPGDVLSSISASTRLAVPDAAEGAGLTQDSAGSALNYTLIDNAVCKADLAGGGG
                     VPVSSGGGAASGASADNGRFGGALGGLLLAPFAFAAMARRRRGRSL"
BASE COUNT          574 a         1124 c         1103 g          514 t
ORIGIN      
        1 caccagcgct cggagatggg ccagcccgaa gcgttcggcg cttcgtgccg caacggcgcg
       61 catccgcccg atccgcagca ctgcaccgac ggcctgggct acgccgacac cgacggcgat
      121 ccgtacaagg gcgactggcg cggcgacttt ccctacaacg tcgaccagct cggcgaaacg
      181 ctgatcgccg actgggacct gggcgcggcc acgctcacct cggtcaccgg ctacatcgac
      241 ttcaagcgca acttccatat cgacaccgat ggctcgccgg ccgacgaatt cgatttcttc
      301 cagaccgaca cggtgaagca gaccacgcag gagctgcgcc tgtccggcgg gcatggcgcg
      361 gcgcagtggc tggccggcgt gttctggagc agggacgaca tccacgtgca cgacgacggc
      421 aggcacgagg atgcgattcc cggcgagttc tccaccatcg aggcgctgca gggcacgcgc
      481 tcggcggccg cgttcggcaa tgtcgactgg aagctgtccg agcgctggac cctgaccagc
      541 ggcctgcgct acacgcacga gtcgcgcgat tacagcggcg gcacgcactg gaccgtgccg
      601 gtgccgggca ccatcgcctc gacttccacc gattcctcga tccacgacag caactggagc
      661 tggaagctcg gcgccagctt cgcacccacg cacacctcgc tgatctacgc caacgcgtcg
      721 aagggcgtga agagcggcgg ctacttctcg ggcgtcacca ccgatagcgc gcagctgcag
      781 ccctacctgc ccgagcaact ggtcgcctac gagatcggcg ccaagacgca gggcgcgttc
      841 gcgctcaacg cgagcgtctt ctactacgac tacaagaacg tgcagacctt catgcgcaac
      901 gcctcggcgc cggtgcagct gatcggcaat gtgcccaagg ccagcaccat ggggctggat
      961 gccgaggcca tcttgcgcgc gctcgacggc ctgacgctgc agaccggcgt gggcctgctg
     1021 cacacgcggc tgggctcgtt cgacggcccg gacggctcgc cggtggccgc cggcaaccag
     1081 ctgcccaatt cgccgcgcat caccttcaac ggcctggcgc gctacgaatt cccgctgttc
     1141 tccgaagacg tgctcggcgc gctgcaggcc gatgcgcact actcggcgtc ggcgttcaag
     1201 gaagccaccg acgacccggt gatccaccag gacccgtact ggatcttcaa cgcgcgcgtc
     1261 ggcgtgctct ccgccagccg cgtgtgggaa gtggcggcct ggggccgcaa cctgaccaac
     1321 aagctctacg tggtgcaggg gctggacgtg ggctcgttgt ttttcggcaa ccgcaactac
     1381 aacgcgccgc gcaccgccgg cgtggaattc agcgtgaagt tctgaggagt tttccatgct
     1441 cgatatcttt ttctacctca ccggcaagag cggcaaggac agcgaactgc gcgcgctgct
     1501 cggcgagatg acgcgcgtgt cgcgcgccga cgacggctgc attcattaca cctttcacca
     1561 gcagctggac gaccggcgca agtgggtgct gcacgagcag tggcgcgacc gcgccgcgct
     1621 cgatgcgcat atcgccagca tgaagaagca tttcggcgag ccgccgccgg gcgcacggct
     1681 gccgcagcgg ctgcatgagc tgcacgagag cagcagttat gcgttccacg aagtgctggc
     1741 atgaacctgc gcaatcgccg ctgggtgctg aagacgcggc cgctgggcat gccggcacgc
     1801 agcgatttcg aactgcgcga agaaccgctg cccgcgctcg ccgacggcca gttcctcgcg
     1861 cgcaacctgt acctgtcgtg cgatcccgcg cagcgcagct ggctggcgcg cgacacctac
     1921 gtgccgaaga tcgccatcgg cgaaaccatg cgcgccggcg ccaccgcgca ggtggtcgaa
     1981 tcgcggcatg cgcagttcaa caccggcgac atcgtctccg gcatgttcgg ctggcaggac
     2041 tacgcggtca gcgacggcag cggctttgtg ccggtgagca agctccccgc cggcgtaccg
     2101 gttccgctgg cgatgagcgc gctgggcctc accgggctca ccgcgtacta cggcatgctt
     2161 gaagtggccg aggtgcgcgc aggccagaac gtgctggtgt ccggtgctgc cggcgctacc
     2221 ggctcggtgg ctgcgcagct ggcgaaaatg cgcggcgcgc gcgtggtcgg cgtggccggc
     2281 ggcgcggcca agtgccagta cctgctcgat caactcaggc tcgacggcgc catcgattac
     2341 cgcgccggcg acgtcggggc gatcatcagg gaaatgtttc cgaaaggcgt cgacatttac
     2401 ttcgacaaca ccggcggcga agtgctggag caggcgctgc tgcggctgtc caccggcgga
     2461 cgcatcgtgc tgtgcggcgc catttccagc tacaacgatc tcgacgccac gccgccgctg
     2521 aagaactgga gcaagctgct ggtcaaccgc gggcgcgtgc agggcatcct ggtcacggat
     2581 ttcgcgccgt acttcgcgcg cgcggccggc gacctggcgc gctgggtggc ggaagggaaa
     2641 ataaagaacc gcgtggacat cgtcgaaggc ctggaacagg cgcccgacgc gctgcgccgc
     2701 ctgttcaccg gcgacaacat cggcaagcag ctggtgaagc tggcggagcc tgctgcaggc
     2761 tgaaaaatcc gttcaccgca ccgccatttc gcctgttact aattatttat ctaaattgtt
     2821 ttcagacaat aattactaac atcggaaatg gcagtgcggt gaggcctttc agaggctgcg
     2881 gccgcgccgc cgacgcgcca tggcagcgaa cgcgaacggc gccagcagca gtccgccgag
     2941 tgcaccgccg aagcggccgt tgtcggccga tgcgcccgac gcggcgccac cgccggagct
     3001 caccggcacg ccgccaccgc cggcgagatc ggccttgcac accgcgttgt cgatcagcgt
     3061 gtagttgagc gcggaaccgg cggagtcctg cgtcagcccc gcgccttccg ccgcgtccgg
     3121 cacggccagg cgcgtcgagg cggagatgct gctcagcacg tcgccgggct tgaggccgat
     3181 ggcggtggtg tcgagcacca gcgtgatgac gccgtcggtg gtgtagttgc tcgaggcgtc
     3241 gagatcaccc aggtcggtga aggtgccgac cggctcgggg ccgggcaacg agccatgcgt
     3301 gccgtacttg aattg
//
LOCUS       DASZZC010000521         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_365270,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000521 DASZZC010000000
VERSION     DASZZC010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365270"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="VHE37_11090"
     CDS             <1..2008
                     /locus_tag="VHE37_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36126.1"
                     /translation="STKLRANHPEVSFMTTATAARDEQMPAGIPFIVVNEFAERFCYY
                     GINSLLVIYMIQFLHFGDARAQAWNATFKSVAYFFPLLGAMVSDIFLAKFRTIIYFSM
                     VYVTGCTVLALGSGETALVLGLFLVGFGTGGIKPCVSTNVGDQFTEKNQHLIERAFSL
                     FYFSINAGSSISIYLCPALLNDPSYGPKIAFGTPAVMMALATIAFWLGRNRFAVVPAA
                     MKNPGYAPIGFIGMFLVMVAVSAAVFAASNYLLALCVMLAQLALAVYVCLNTGLRSAL
                     PGELIAWLQRSFTGDSLRTVGRLSLLYLFIAFFWALWDQSNGNSWTVQAQSALMDKHL
                     FAFMVGFPDWTLYAFFIVAAGGVAAAIGVLVRSFMSLAWTGSVGFALFIIMMYFGWAH
                     VPQMPAYEMLPAQLQVVNGLFILVMIPVFTFVIYPLLGRLFVVTPLRKIGIGFFVCAA
                     SFVLVAWIEQQIQNGTSVSLWWQIAAYAVLTASEILVSITGLEYSYMQAPPVMKSFIM
                     SLFLLSTSVGNAFTATVNDLMVRPLHASAVESGAQTWVTLDAVDTFVVGQKIDLDGDN
                     GVQIVSEDGKSEPLAGTFLIGEIDSVGHRVRLLDKVDRRPVVSSGTYNAAKGSVSTYA
                     LVGPVYFLFFAALMGGIAVVFIAFAVRFKEKTFVREDAPATAAA"
     gene            2012..>2356
                     /locus_tag="VHE37_11095"
     CDS             2012..>2356
                     /locus_tag="VHE37_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36127.1"
                     /translation="MRGAVGALFAMLAASAAAQPPAAGIGDDDLVQMQQAAAEYLHGN
                     LLQVGAVSPSCRCEDGTDCSASVPLVYRTTQGPRTLQLVKVRERWQVSALDKAQLALA
                     VLKREYAREAHGA"
BASE COUNT          371 a          724 c          739 g          522 t
ORIGIN      
        1 ctcaactaaa cttcgtgcaa accacccgga agtttccttc atgaccaccg ccaccgccgc
       61 ccgcgacgag cagatgcccg ccggcatccc gttcatcgtc gtcaacgaat tcgccgagcg
      121 cttctgctac tacggcatca actcgctcct ggtcatttac atgatccagt tcctgcattt
      181 tggcgacgcc agggcgcagg cgtggaatgc aacattcaag tccgtggcgt atttctttcc
      241 gttgctgggc gccatggtct cggacatctt cctggccaag ttccggacca tcatctattt
      301 ttcgatggtg tacgtgaccg gctgcacagt actcgcgctc ggcagtggtg agaccgcgct
      361 ggtactcggc ttgtttctgg ttggcttcgg cacgggcggt atcaagcctt gcgtctcaac
      421 caacgtcggc gaccagttca ccgagaagaa ccagcacctg atcgagcgcg cgttttcgct
      481 gttctatttt tcgatcaacg ccggttcgtc gatctccatc tacctgtgcc cggcccttct
      541 caacgatcct tcatacggcc cgaaaatcgc cttcggcact ccagcagtga tgatggcgct
      601 ggccaccatc gctttctggc tgggacgcaa tcgctttgca gtggtgcctg cggcgatgaa
      661 aaaccccggc tatgccccga ttgggttcat cgggatgttc ctggtgatgg tcgcggtttc
      721 ggctgcggta ttcgccgcga gcaattacct gctcgccttg tgtgtgatgc tggcgcagct
      781 ggcgctggcg gtttatgtct gcctgaacac cgggctgcgc tcggcgctgc cgggagagtt
      841 gatcgcgtgg ctgcagcgtt cgttcaccgg cgacagtttg cgcacggtcg gccggctgtc
      901 gctgctgtac ctgttcattg catttttctg ggcgctttgg gaccagtcga acggcaacag
      961 ctggacggtg caggcacaga gtgcattgat ggacaagcac ctgttcgcct tcatggtggg
     1021 gttccctgac tggactttgt atgcgttctt catcgtcgcg gccggcggtg tggccgctgc
     1081 catcggcgtg ctggtgcgca gtttcatgag cttggcctgg acgggttccg tcggcttcgc
     1141 actttttatc atcatgatgt atttcggctg ggcacatgtc ccgcaaatgc cggcttacga
     1201 aatgctgccg gcgcaattgc aggtggtgaa cggcttgttc atcctggtga tgatcccggt
     1261 cttcaccttc gtcatctacc cgctgctggg ccgcctgttt gtcgtcacgc cgctgcgcaa
     1321 gatcggcatc ggcttttttg tctgcgccgc ttccttcgtc ctggtggcgt ggatcgagca
     1381 gcagattcag aacggcacca gcgtttcgtt gtggtggcag atcgcggcct atgccgtgct
     1441 gacggcttcc gagatcctgg tatcgatcac cgggctggaa tacagctaca tgcaggcgcc
     1501 accggtgatg aagagcttca tcatgagcct gttcctgctg tcgaccagcg tcggcaatgc
     1561 gttcaccgca accgtaaacg atctgatggt gcgcccgctt cacgccagcg cggtggaatc
     1621 cggcgcccag acctgggtga cgctggatgc ggtggacaca ttcgtggtcg gccagaagat
     1681 cgatcttgat ggcgacaacg gcgtgcagat tgtcagcgaa gacggcaaga gcgagccgct
     1741 ggccggcact ttcctcatcg gcgagatcga cagcgtgggg catcgcgtgc gcctgctcga
     1801 caaggtggat cgccggccgg tggtgtccag cggcacctac aacgcggcca agggttcggt
     1861 ctcgacctac gcgctggtcg gcccggtgta cttcctgttc tttgccgcgc tgatgggtgg
     1921 catcgccgtg gtgttcatcg ccttcgccgt gcgcttcaag gaaaagacct tcgtgcgcga
     1981 ggacgcgccg gccacggcgg ccgcgtaatt catgcgcggc gcggttggcg cgctgttcgc
     2041 gatgctcgcg gcatcggccg cggcgcagcc gcccgcagcc ggcatcggcg acgacgacct
     2101 ggtgcagatg cagcaggccg ccgccgagta cctgcatggc aacctgctgc aggtaggcgc
     2161 agtgagcccc tcgtgccgct gcgaagatgg gactgattgc agcgccagcg tgccgctggt
     2221 ctaccgcacc acgcagggtc cgcgcacgct gcagctggtg aaggtgcgcg aacgctggca
     2281 ggtgagcgcg ctggacaagg cgcagctcgc gctcgccgtg ctcaaacgcg aatacgcgcg
     2341 cgaggcgcac ggcgcc
//
LOCUS       DASZZC010000522         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1258295,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000522 DASZZC010000000
VERSION     DASZZC010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258295"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VHE37_11100"
     CDS             <1..182
                     /locus_tag="VHE37_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36128.1"
                     /translation="ITGDSCSNAALPAGSSCSILLRFAPTQVGALDASILVQTSDSRH
                     ATELIRLHGEGQASE"
     gene            complement(223..1161)
                     /locus_tag="VHE37_11105"
     CDS             complement(223..1161)
                     /locus_tag="VHE37_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT36129.1"
                     /translation="MPRARRIMVLVDRRSRGGGALRRAVQLARTGSAELHLCLVEPAA
                     GARSALQRNVLVLARSAQRQEGHDWLLDLADEVKMLAGPVHTHLVLDQPLHLRLLERI
                     DTLRPDLLVMSLHYQPQLKRSLLTPLAWTLLRQAPCDLLLAGRALGDALARVIAVVDT
                     AHVDAHHSELNRHTLALARSLADQSGAELHLVQAGAQVVAPDSLPALAAEARHLHELQ
                     KRRFAQFAEENQVPSRLRHLLAGDPATALSRFASKPASDLVMLGVSGEPGEQPLPEYL
                     IGQLRCDVWVVRAPASMTARPAAPAIQPPDELMSLN"
     gene            1149..>2535
                     /locus_tag="VHE37_11110"
     CDS             1149..>2535
                     /locus_tag="VHE37_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HVT36130.1"
                     /translation="MRAALPAPAWKMAPACAGAARVALICINAAGDANTYRGAMDNPA
                     SAAAARTAGALRSSALVRLRRMGIDTYQSPVIYMRADCGVCRSEGFEAQSRVEIRRGS
                     DIIVATLNVMHDGLLAGDEAGLSEAAWRLLRAGEGDTAELRHPDPLDSLSALRAKVYG
                     SELNLAQLRGIVNDITAGRYSDLELAAFVTACAGARMSLAETVALTRAMVDAGETLRW
                     PHAQVVDKHCIGGLPGNRTTPILVPIVAACGLCIPKTSSRAITSPAGTADVLETMTAV
                     ELDLPTLRRVVEAEGGCLAWGGSVALSPADDALIRVERPLDFDSESQVVASVLSKKIA
                     AGATHVLIDIPVGPTAKVRSAAAAQVLSRRLLEVGRELNISVTPLFTDGSQPVGRGIG
                     PALEARDVLAVLRAEPGAPRDLRERALQLAAQVLELSPEIARGQGMARARAALDSGVA
                     WNKFLAICRAQG"
BASE COUNT          358 a          882 c          919 g          376 t
ORIGIN      
        1 tgatcaccgg cgacagctgc agcaatgcag ccctgcccgc cggcagcagc tgcagtattt
       61 tgctgcgctt cgcgccgacc caggtgggcg cgttggacgc cagcatcctg gtgcagacca
      121 gtgactcccg gcatgccacc gaactcatcc ggctccatgg agaggggcag gcctcggaat
      181 gaggtcgatg cggcaggcgc ctgatcgcgc ctgccaccag tgtcagttca gcgacatcag
      241 ttcgtcaggc ggctggatgg ccggggctgc cggacgcgcg gtcatcgatg ccggtgcccg
      301 cacgacccac acgtcgcagc gcaactggcc gatgaggtat tcgggcagcg gctgctcgcc
      361 cggctccccc gacacgccca gcatgaccag gtccgatgcc ggcttgctgg cgaagcggga
      421 cagcgcagtc gccggatcgc cggccagcag gtgacgcagc cgggacggca cctggttctc
      481 ctcggcaaat tgcgcgaacc ggcgtttctg cagttcgtgc agatgccgcg cctccgcggc
      541 cagtgccggc agcgaatcgg gcgccacgac ttgcgcgccc gcctgtacga ggtgcagttc
      601 cgctccgctt tggtccgcga ggctgcgcgc cagcgcgagc gtgtggcggt tgagttccga
      661 atggtgtgca tcgacgtgcg cggtgtcgac caccgcgatg acgcgtgcca gcgcatcgcc
      721 gagagcgcgt ccggccagca gcaggtcgca cggcgcctgg cgcagcagcg tccaggccag
      781 tggtgtcagc agtgaccgct tgagctgcgg ctggtaatgc agcgacatca ccagcaggtc
      841 cggccgcagg gtgtcgatgc gttccagcag ccgcaggtgc agcggctggt ccagcaccag
      901 gtgcgtgtgc accggcccgg ccagcatctt cacctcgtcg gcgaggtcga gcagccagtc
      961 atggccttcc tggcgttgtg cactgcgcgc cagtaccagc acgttgcgct ggagcgcgga
     1021 gcgcgcaccg gcggccggtt cgaccaggca caggtgcagt tcggctgaac cggtgcgtgc
     1081 cagctgcacc gcacggcgca gcgcgccgcc gccgcggctg cggcggtcga ccaggaccat
     1141 gatgcggcgt gcgcgcggca ttgcccgctc ctgcgtggaa gatggcgcca gcttgcgccg
     1201 gcgcggcgcg cgtcgcgttg atctgcatca acgctgccgg cgatgcgaac acctaccgtg
     1261 gcgccatgga caacccggcc agcgcagcag cggcgcgcac cgcgggcgcg ctgcgctcgt
     1321 cggcgctggt gcgcctgcgg cgcatgggca tcgacaccta ccagtcaccg gtgatctaca
     1381 tgcgcgccga ctgcggcgtg tgccgctcgg aaggtttcga ggcgcagtcg cgggtcgaga
     1441 tccgccgggg cagcgacatc atcgtggcca ccctcaatgt catgcatgac ggcctgcttg
     1501 ccggcgacga agccggcctg tcggaggccg cgtggcgcct gctgcgtgcg ggcgagggcg
     1561 ataccgcgga actgcgtcat cccgatccgc tcgattcgct gtcggcgctg cgcgccaagg
     1621 tctacggcag cgagctcaac ctggcgcagc tgcgcggcat cgtcaacgac atcaccgccg
     1681 gccgctattc cgacctggag ctggccgcgt tcgtcaccgc ctgcgccggc gcgcgcatga
     1741 gcctggcgga aacggtggcg ctgacgcgcg ccatggtcga cgccggcgaa acgctgcgct
     1801 ggccacacgc gcaggtggtg gacaagcact gcatcggcgg cctgccgggc aaccgcacca
     1861 cgcccatcct ggtgccgatc gtcgccgcct gcgggctgtg catcccgaag acctcgtcgc
     1921 gtgccatcac ctcgcctgcc ggcaccgctg acgtgctgga gaccatgacc gcggtggaac
     1981 tcgacctgcc gaccctgcgc cgcgtggtcg aagcggaagg cggctgcctg gcctggggtg
     2041 gttcggtggc gctgtcgccg gcggacgatg cgttgatccg cgtcgagcgg ccgctggatt
     2101 tcgacagtga gtcgcaggtg gtggccagcg tgctgtcgaa gaagatcgcc gccggcgcca
     2161 ctcacgtgct gatcgacata ccggtcgggc cgacggccaa ggtgcgcagc gccgccgcgg
     2221 cgcaggtgct gtcgcggcgc ctgctcgaag tcggccgcga gctgaacatc agcgtgacgc
     2281 cgctgttcac cgacggcagc cagccggtgg gccgcggcat cgggcccgcg ctggaagcgc
     2341 gcgatgtgct ggccgtgctg cgcgccgaac ccggcgcgcc gcgcgatctg cgtgagcgcg
     2401 cgctgcagct ggcggctcag gtgctggaac tgtcgccgga gattgcgcgc gggcagggca
     2461 tggcgcgcgc gcgcgccgcg ctggattccg gcgtggcctg gaacaagttc ctcgccatct
     2521 gccgcgcgca gggcg
//
LOCUS       DASZZC010000523         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_808541,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000523 DASZZC010000000
VERSION     DASZZC010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808541"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1200
                     /locus_tag="VHE37_11115"
     CDS             166..1200
                     /locus_tag="VHE37_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HVT36131.1"
                     /translation="MSTPASLDANEFRRALGSFATGVTIITTRAPDGTPLGLTCNSFN
                     SVSLSPPLVLWSLANTSASLDAFRATQHWAVHVLAADQEELSARFARRGIDKFAGLEC
                     DIGAGNIPLLRGCSARFQCRTAFQQEGGDHIIFVGEVLAFDRADSAPLVFHGGRYAHA
                     TKREPAAAQPRAAYLAGSFSDDFLGYLLGRSHFQFFTQIRPHLQAEGLSDEQFYVLST
                     LTLKRAMSSDEMDAAMAGILGAGSGETVQALVAHGLARIAPVVAEVHGTEYELTDRGR
                     EVALRVIAAAKAVELRLIEGLGADDAAALKTLLRRLLGAIDPASRAIWERSGKPPLQM
                     PNPAGSDWLE"
     gene            complement(1207..1914)
                     /locus_tag="VHE37_11120"
     CDS             complement(1207..1914)
                     /locus_tag="VHE37_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36132.1"
                     /translation="MEKVIYVLWKDAAADGAAWMQRLRSELAPRLRALPCVHGVKLNL
                     CDADVAPAAALRQQHTQPQPDALVQLWLDSAIAHLRAEVDRAVGAHAQKSAAWLVTES
                     QPLRNSKHAPRPGERTYGFAQVALLKKPEKLPYADWLDVWHNSHTQVAVETQSTFEYI
                     QNVVVRALTPGAPAWDAMVEECFPPEAMADPYTFFDARGDEAKFKRNLQRMMDSVQRF
                     IELGRLDVLPTSQYRYD"
     gene            complement(2066..2857)
                     /locus_tag="VHE37_11125"
     CDS             complement(2066..2857)
                     /locus_tag="VHE37_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36133.1"
                     /translation="MLLKDKVVIVSGIGPGLGSKLAIEAAREGARVVMGARTASKLDD
                     AEKAIREQGLKTELLKQVCDITDVKSCEAIVSAAIKKFGRIDGLVNSAFVHGDMDYPS
                     TAKLDDWISGPIATNLIGTLKLTQAVIPQMKQQGGGAIVMINTMAVRTVPPLGEAGYA
                     ASKAALANSAKYLAKELGPHKIRVNTVHMGWMWGAPVIGYMQWQSQQTGVPVEQMKAG
                     IAAGIPLGDIPTDDDCAKPALFLISDYARVVTGATLDVNGGHYMP"
     gene            complement(2861..>3068)
                     /locus_tag="VHE37_11130"
     CDS             complement(2861..>3068)
                     /locus_tag="VHE37_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVT36134.1"
                     /translation="MDVVQHVYRFIGWPLTLALERTLYNWLEADDRKHQAGGPHDYTP
                     EQFGLSAEQIRQDFAAYRKQHIEE"
BASE COUNT          494 a         1047 c          985 g          542 t
ORIGIN      
        1 gttctcgctc ggctacaatt tcttcttcgg caacccggac aagcgcgagc gcgacgtcag
       61 cccggtggag cagaaccctt actccgaccg cgacaacatc accttcacgg tcaaatacaa
      121 catttgaggg agtcgcagcg ctgcggcgcg ggttagcatt tggtcatgag cacacccgcg
      181 tcgctcgacg ccaacgaatt ccgccgagcc ctcggctcgt tcgccaccgg cgtcaccatc
      241 atcaccacgc gcgcgcccga cggcacgccg ctgggcctga cctgcaacag tttcaactcg
      301 gtgtcgctgt cgccgccgct ggtgctgtgg agcctggcca acacctcggc cagcctcgac
      361 gccttccgcg ccacccagca ctgggcggtg cacgtgctgg cagctgacca ggaagaactg
      421 tcggcgcgat tcgcccgccg cggcatcgac aagttcgccg ggctggagtg cgacatcggt
      481 gccggcaaca tcccgctgct gcgcggctgc agcgcgcgtt tccagtgccg caccgcgttc
      541 cagcaggaag gcggcgacca catcatcttc gtcggcgagg tgctggcatt cgaccgcgcc
      601 gacagcgcgc cgctggtgtt ccacggcggc aggtacgcgc atgccaccaa acgcgagccg
      661 gcggcggcgc agccgcgcgc ggcatatctc gccggcagtt tctccgacga ttttctcggc
      721 tacctgctgg gccgcagtca tttccagttc ttcacccaga tccgtccgca cctgcaggcg
      781 gaaggcctct ccgacgaaca gttctacgtg ctgtccacgc tcacgctcaa gcgcgccatg
      841 agcagcgacg agatggacgc ggccatggcc ggcattctcg gcgcgggcag cggcgagacg
      901 gtgcaggcgc tggtcgccca cggcctggcg cgcatcgcgc cggtggtggc ggaggtgcat
      961 ggcaccgagt acgaactcac cgaccgcggg cgcgaagtgg cgttgcgcgt gatcgccgcg
     1021 gccaaggcgg tggaactgcg cctgatcgaa ggcctgggcg cggacgatgc cgccgcgctg
     1081 aagaccttgc tgcgccgcct gctcggcgcc atcgatcccg cgtcgagagc catctgggag
     1141 cgcagcggca agccgccgct gcagatgccc aacccggccg gcagcgactg gctggaatag
     1201 gcgcgctcag tcgtagcgat actggctggt cggcagcacg tccagccggc ccagctcgat
     1261 gaaacgctgc acgctgtcca tcatgcgctg caggttgcgc ttgaacttcg cttcatcccc
     1321 gcgtgcgtcg aagaaggtgt agggatcggc catcgcctcc ggcgggaagc attcctcgac
     1381 catcgcatcc caggccggtg cgcccggggt gagcgcgcgc accaccacgt tctggatgta
     1441 ttcgaaggtg gactgcgtct cgacggcgac ctgcgtgtgc gagttgtgcc acacatcgag
     1501 ccagtccgcg tacggcagct tctccggctt cttcagcagt gctacctggg cgaaaccgta
     1561 agtgcgctcc ccgggacgcg gcgcgtgctt cgaattccgt agcggctgcg attcggtgac
     1621 cagccaggcg gcggacttct gcgcgtgcgc gcccacggcg cggtcgactt ccgcgcgcag
     1681 gtgcgcgatg gcgctgtcga gccacagctg caccagcgcg tcgggctgcg gctgcgtgtg
     1741 ctgctggcgc agcgcggcgg caggcgcgac gtcggcgtcg cagagattga gcttcacgcc
     1801 gtgcacgcag ggcagcgcgc gcaggcgcgg cgcgagctcg ctgcgcaggc gctgcatcca
     1861 cgcggcgcca tcggcagcgg cgtccttcca cagcacgtag atgacttttt ccatcggccc
     1921 agctccgaaa aatgcctgca ccgcaccacg aatttacctg ttataaaaaa tctataaaat
     1981 cttaaaacga taaataatta ataacagtcg aaatggcggt gcggtggagc aaaaaaatgc
     2041 cggctcacgt ggtgcaacag cgcgctcacg gcatgtagtg gccaccgttg acgtcgagcg
     2101 tggcgccggt gaccacgcgc gcgtagtcgg aaataaggaa cagcgcgggc ttggcgcagt
     2161 catcgtcggt gggaatgtcg cccagcggaa tgcccgcggc aatgcccgcc ttcatctgct
     2221 ccaccggcac gccggtctgc tgcgactgcc actgcatgta gccgatcacc ggcgcgcccc
     2281 acatccagcc catgtgcacg gtgttgacgc ggatcttgtg cgggcccagt tccttggcca
     2341 ggtacttggc cgagttggcc agcgccgcct tcgacgcggc atagccggct tcgcccagcg
     2401 gcggcaccgt gcgcaccgcc atcgtgttga tcatgacgat ggcaccgccg ccctgctgct
     2461 tcatctgcgg gatcaccgcc tgcgtcagct tgagcgtgcc gatcaggttg gtggcgatgg
     2521 ggccgctgat ccagtcgtcg agcttggccg tgctcgggta gtccatgtcg ccgtgcacga
     2581 aggcgctgtt gaccaggccg tcgatgcggc cgaatttctt gatcgccgcg ctgacgatcg
     2641 cctcgcagct cttcacatcg gtgatgtcgc acacctgctt gagcagctcg gtcttcaggc
     2701 cctgttcgcg gatggccttt tcggcgtcgt ccagcttgga cgcagtgcgc gcgcccatga
     2761 ccacgcgcgc gccttcgcgc gccgcctcga tggccagctt ggagccgagc cccgggccga
     2821 tgccggaaac gatcaccacc ttgtccttga gcagcatgtg ttactcctcg atgtgttgtt
     2881 ttcggtacgc cgcaaaatcc tgcctgatct gctccgccga caggccgaac tgttccggcg
     2941 tgtagtcgtg cggcccgccc gcctggtgct tcctgtcgtc cgcttccagc cagttgtaca
     3001 gggtgcgctc cagtgccagc gtcagtggcc agccgatgaa gcggtacacg tgctgcacca
     3061 cgtccatc
//
LOCUS       DASZZC010000524         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_103579,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000524 DASZZC010000000
VERSION     DASZZC010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103579"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VHE37_11135"
     CDS             <1..741
                     /locus_tag="VHE37_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVT36135.1"
                     /translation="AQPRDRPLSAQTVNHITGLLNMIMTEASIRYGFPNPCTAMKRLK
                     LRRGDIEPFTLQEAKKIIAAVREDFRNYFTLRFFTGMRTGELHGLKWSHIDYERRQIL
                     VRETFAKGRTDYTKTDGSQRDIDMSGVVMQALKAQELLTKSRSEYVFCTRAGTPLDEK
                     NVTDRVWYPLLRHLQLKKRRPYQCRHTCATFWLASGENPEWIARQMGHTTTEMLFRTY
                     SRYVPNLTRKDGSAFDRMLEAAMVQAPQ"
     gene            complement(1001..1077)
                     /locus_tag="VHE37_11140"
     tRNA            complement(1001..1077)
                     /locus_tag="VHE37_11140"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1041..1043),aa:Pro,seq:cgg)
     gene            complement(1094..2062)
                     /locus_tag="VHE37_11145"
     CDS             complement(1094..2062)
                     /locus_tag="VHE37_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVT36136.1"
                     /translation="MELKNILAPVADDLKRVDAMILERLRSQVSLINEVGNYLVSAGG
                     KRMRPAMVLLVARALGCTGEAPILLAATIEFIHTATLLHDDVVDGSGLRRSRKTANAL
                     WGNSAAVLSGDFLYSRSFQMMVDAGSMDIMRVMADTTNAIAEGEVLQLVNCGDPDVDQ
                     ARYLRVIELKTARLFEAGARTGAICAGADMAQRERAAAYGRKLGIAFQLIDDVLDYCA
                     DPAVTGKNIGADLAEGKPTLPLIRAMAQAPAEQAETIRDAIAHARVDRLNEVLKIVES
                     TDAIPYTRALAERHGREAVDAIGEVPASPYKDALIELARYNNERGS"
     gene            2249..3397
                     /locus_tag="VHE37_11150"
     CDS             2249..3397
                     /locus_tag="VHE37_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT36137.1"
                     /translation="MTIDHSELELFRDNVVRFLKDEVEPGYDAWEKSGITPRTLWNKL
                     GEAGLLCLDVPEAYGGIGAPFEFSCVMLEEVGRMGFGALSANLAVHSDIVAPYILHLG
                     SEEQKKKFLPKMVTGECVGAVAMTEPGAGSDLQGIKTSALPDGNGGYVINGSKTFITN
                     GQHADVVVTFTKTDPKAGAKGTTLFLVERSMPGFTRGRNLEKLGQHCGDTSELFFNDV
                     KVPASAILGRMGNGFGHLIDELPRERLTLAVHAVSAAEGALARTIEYVRSRKAFGTTV
                     AAFQNTRFELADMKTEIEVNKAYVKHCAQLYAQGKMDVPTAAMVKLSTTEMQYRVCDR
                     CLQLFGGYGYMTEYPISRYFADARVQRIYGGTSEIMKEVIARSIIGRG"
     gene            complement(3525..3977)
                     /locus_tag="VHE37_11155"
     CDS             complement(3525..3977)
                     /locus_tag="VHE37_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36138.1"
                     /translation="MNLDISRLSAHELDDLIRAAAERRAEMEPSQPENPPIEVTAMMD
                     PRWRLRSYGADTLLQLRDPGHGWLSFLLPPHERVNILTWLLTQALGPQAHTPGTALAA
                     PAAGGGQLHLPDVTVTGAQIKPLVTVKKVGTAKKPAPTKKPAPTKKKR"
     gene            complement(3997..4392)
                     /locus_tag="VHE37_11160"
     CDS             complement(3997..4392)
                     /locus_tag="VHE37_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVT36139.1"
                     /translation="MDYALTTVVTVLSLILYFVLTFNVGRARGKHKIDAPAMTGHPEF
                     ERVLRVQMNTLEQLALYLPGLWLFSIYVSSIWGAVLGGVWIVGRVLYARGYYVEAGKR
                     SAGFLVSIGATGLLLLGALLGAIYAMVQG"
BASE COUNT          832 a         1501 c         1469 g          784 t
ORIGIN      
        1 gcccagcccc gggaccgccc cctcagcgca cagacggtca accacatcac cggcctgctg
       61 aacatgatca tgacggaggc atcgatccgc tatggttttc ccaatccctg cacggcgatg
      121 aaacgactca agttgcgcag aggggacatt gaacccttca ccctgcagga ggccaagaag
      181 atcatcgcgg cggtccgcga ggatttccgg aattacttca ccttgcgctt tttcaccggc
      241 atgcgcacgg gcgagctgca tggcctgaag tggtcgcata tcgactacga gcgtcggcaa
      301 atcctggtgc gggagacgtt tgccaagggt cgcactgact acaccaaaac cgacggctcc
      361 cagcgggaca tcgacatgtc gggcgtggtc atgcaggcgc tcaaagcgca ggaactcttg
      421 acgaagtcga ggtcggagta cgtgttctgc acgcgcgcag gcaccccgct cgacgaaaag
      481 aacgtcactg accgagtctg gtatccgctg ttacgccatc tccagttgaa gaagcgccgg
      541 ccgtaccagt gccgccatac gtgcgcaacg ttctggctgg cctcaggcga aaacccggag
      601 tggattgcgc gccaaatggg ccacaccacg acggaaatgc tgtttcggac ctatagccgg
      661 tacgtaccca acctcactcg aaaggatgga tcggcgttcg atcgcatgct agaagcggcg
      721 atggtgcagg cgccgcagta agcggccgcg gttatccagg ggtgagactt gtggggaggg
      781 tatcgccgcc ctatcagcgc gctgacgatg gccgcggtgg gcacagcgtg ggcacaggct
      841 gtgcccattt ctgtgcccaa acagccaaag ttttgaccgg ctccggcagc agcaagaggt
      901 caaacgtgtt ttgggcacaa atgggcacag ttttgggcac atgtgcccaa aaaatgtctc
      961 gcgttaaatc tggaaatgtg ccgtaaccaa ctgatttaat tggtcggggc gacaggattc
     1021 gaacctgcga cctcttgctc ccgaagcaag cgctctacca agctgagcta cgccccgcgc
     1081 cttgcggaaa acctcagctg ccgcgctcgt tgttgtagcg ggccagctcg atcagcgcgt
     1141 ccttatatgg tgaggcggga acctcgccga tcgcgtcgac cgcttcacgg ccgtgccgtt
     1201 cagcgagcgc gcgagtgtac gggatcgcat cggtcgattc aacgattttc agcacctcgt
     1261 tcagccggtc cacgcgcgca tgcgcgatgg cgtcgcggat cgtctccgcc tgctccgccg
     1321 gcgcctgcgc catggctcgg atcagcggca gcgtgggctt gccttcggcc aggtcggcgc
     1381 cgatgttctt gccggtcacc gccgggtcgg cgcagtagtc gagcacgtcg tcgatcagct
     1441 ggaaggcgat gccgagcttg cggccgtatg ccgccgcgcg ctcgcgctgc gccatgtccg
     1501 cgcccgcgca gatggcgccg gtgcgcgcac ccgcttcgaa cagccgcgcg gtcttgagct
     1561 cgatcacgcg caggtagcgc gcctggtcca cgtcaggatc gccgcagttc accagctgca
     1621 gcacctcgcc ctcggcgatg gcgttggtgg tgtcggccat cacccgcatg atgtccatgc
     1681 tgccggcgtc caccatcatc tggaagctgc gcgagtagag gaaatcgccc gacagcaccg
     1741 cggcggaatt gccccacagc gcattggccg tcttgcggct gcggcgcagg ccggagccgt
     1801 cgaccacgtc gtcgtgcagc agcgtggcgg tgtggatgaa ctcgatggtc gcggccagca
     1861 ggatcggcgc ctcgccggtg cagcccagcg cgcgcgccac cagcagcacc atggccgggc
     1921 gcatgcgctt gccgccggcc gagaccaggt agttgccgac ttcgttgatc aggctcacct
     1981 gcgagcgcag gcgctcgagg atcatggcgt cgacgcgctt cagatcgtcg gcgaccgggg
     2041 cgaggatgtt cttgagttcc atgaatgggg accgcgaggg cggcgcgatg ctagaagcag
     2101 agggcggtga cgtcaatgcg cggatcgacc cgcgccgcgc gcgccctccc ctttgagcag
     2161 ggctgtcggg cacagccgca cgctagaatg cgcgcccgaa aatctcccgc gcggagtttt
     2221 tcgccgttct ctcctgacga tcagacccat gaccatcgac cacagcgaac ttgaactctt
     2281 ccgcgacaac gtcgtccgct ttctcaagga cgaggtcgag cccggctacg acgcctggga
     2341 aaaatccggc atcacgccgc gcacgctgtg gaacaagctg ggcgaggccg gcctgctctg
     2401 cctggacgtg ccggaagcgt acggcggcat cggcgcgccg ttcgagttct cctgcgtgat
     2461 gctcgaagaa gtcggccgca tgggcttcgg cgcgctgtcg gccaacctcg cggtgcactc
     2521 ggacatcgtc gcgccctaca tcctgcacct gggctccgag gagcagaaga agaagttcct
     2581 gcccaagatg gtcaccggcg aatgcgtcgg cgccgtcgcc atgaccgagc ccggcgcagg
     2641 ctccgacctg cagggcatca agaccagcgc gctgccggac ggcaacggcg gctatgtgat
     2701 caacggctcc aagaccttca tcaccaacgg ccagcacgcc gacgtggtgg tcacgttcac
     2761 caagaccgac cccaaggccg gcgccaaggg cacgacgctg ttcctggtcg agcgctcgat
     2821 gcccggcttc acccgcggac gcaacctgga gaagctgggc cagcattgcg gcgacacctc
     2881 ggaactgttc ttcaacgacg tgaaggtgcc ggcctccgcc attctcggcc gcatgggcaa
     2941 cggcttcggc catctcatcg acgaactgcc gcgcgagcgc ctgacgctgg ctgtgcatgc
     3001 ggtcagcgcc gcggaaggcg cgctggcgcg caccatcgag tacgtgcgca gccgcaaggc
     3061 cttcggcacc acggtggcgg cgttccagaa cacgcgcttc gaactggccg acatgaagac
     3121 cgagatcgag gtgaacaagg cctacgtgaa gcactgcgcg cagctctacg cgcagggcaa
     3181 gatggacgtg ccgacggcgg ccatggtgaa gctctccacc accgagatgc agtaccgcgt
     3241 gtgcgaccgc tgcctgcagc tgttcggcgg ttacggctac atgaccgagt atcccatcag
     3301 ccgctacttc gccgacgcgc gcgtgcagcg catctacggc ggcacctcgg aaatcatgaa
     3361 ggaagtgatt gcgcgttcca tcatcggccg cggctgatcg aaccgctcga aacgcgcgcg
     3421 acaggcctca ccgcactgcc ggtaccgatg ttataaaaaa ttgtctgtac ttaatttaat
     3481 acagcaaata ataacaacgg aaatcggcat gcggtggcag gttttcagcg cttcttcttc
     3541 gtcggagccg gcttcttcgt cggcgccggc ttcttcgcgg tgcccacttt tttcaccgtg
     3601 accaacggct tgatctgcgc gccggtcacg gtcacatccg gcaggtgcag ctggccgccg
     3661 cccgccgccg gcgcggccag cgcggtgccg ggcgtatgcg cctgagggcc cagcgcctgc
     3721 gtcagcagcc aggtgaggat gttcacgcgc tcatgcggcg gcagcaggaa actgagccag
     3781 ccatggccgg ggtcgcgcag ctgcagcagg gtgtcggcgc cgtaggagcg caggcgccag
     3841 cgcggatcca tcatcgcggt gacttcgatg ggcggatttt ccggctgcga cggctccatc
     3901 tccgcccgcc gctcggcggc ggcgcggatc aggtcgtcga gttcgtgtgc ggacagccgg
     3961 ctgatgtcga ggttcatatg tgcgctccgg atggcttcag ccctggacca tggcgtagat
     4021 ggcgccgagc agcgcgccca gcagcagcag gccggtagca ccgatggaaa ccaggaaacc
     4081 ggcgctgcgc ttgccggcct ccacgtagta gccgcgcgca taaaggacgc gcccgacgat
     4141 ccacacgccg cccagcaccg cgccccagat ggacgacacg tagatcgaga acagccacag
     4201 gcccggcagg taaagcgcca gctgctccag cgtgttcatc tgcacgcgca gcacgcgctc
     4261 gaactccggg tgcccggtca tcgccggcgc gtcgatcttg tgcttgccgc gcgcgcgtcc
     4321 tacgttgaag gtgaggacga agtacaggat cagcgacagc acggtaacga cggtggtcaa
     4381 ggcgtaatcc atggggctcc ctttgaactg atgctggttg ttgttgcgct caatcgtatt
     4441 ccattccggc cgccccgcgc cgcacggtga agccggcggc ctgcaggtcc gacacgatct
     4501 ggttggcatg gctctcgtcc tgcgcctcca ccagcacttc cagcgtcgcg ttcttcgcgt
     4561 gcgtacccgc atgcaggcgt tcgtga
//
LOCUS       DASZZC010000525         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1137180,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000525 DASZZC010000000
VERSION     DASZZC010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137180"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /gene="ggt"
                     /locus_tag="VHE37_11165"
     CDS             complement(<1..1543)
                     /gene="ggt"
                     /locus_tag="VHE37_11165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVT36140.1"
                     /translation="MKTLALALAMTSIAASAGPDRVTGLSFATRAEVIGQHGMAATSQ
                     PLATQIALDVLKRGGSAVDAAIAADAALGLMEPVGAGIGGDLFAIVWDPKTQKLYGLN
                     ASGRSPKSLTLEYFQQQGLKHIPPLGPLPVTVPGCVDGWFELHGKFGRLPMKDDLAPA
                     IGYARNGFPLSELIAFYWDKSVPRLSKFPGFAEQMTINGRAPRKGEIWKNPNLAHTLE
                     TIARGGRDAFYKGDIAHVIDQYIHQQGGFLSYGDFAAHHSDWVEPVSTSYRGYDVWEL
                     PPNSQGIAALQILNVLEGFDIGKFGFGSADHMHAYVEAKKLAFEDRAKYYADPAFNAL
                     PVAGLIDKKYADGRRKLLDMNHAARRYDAGNPAMKQGDTVYLTTADSSGMMVSLIQSN
                     YRGMGSGMTPPGLGFVLQDRGEMYSLTPGHVNVYAPGKRPFQTIIPAFITKDGKPWVS
                     FGVMGGDMQPQGHAQIVMNLVDFHMNLQEAGDAPRMYHTGSSEPTDEVMTDGGELHLE
                     SGFPYE"
     gene            complement(1617..2924)
                     /locus_tag="VHE37_11170"
     CDS             complement(1617..2924)
                     /locus_tag="VHE37_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36141.1"
                     /translation="MTARTFIMTALLAPLMVTAARAGDPPAAAAGGDEFICSTFPGGC
                     TPAEVRYYRCKKYPETCDEHASGSPRPVSAPPASSPPPALLAPACREGDAGCAVQARV
                     APPQPAPQPQAPPSPPAEAAAAAPPAPVIAVEHPAVEHPAPADEPEQPHLESIEREDT
                     AVAAPAAPPPAAATAARPNATEQRLKARSDRLQAQIDQQQQELARLQARQQQVTQLRE
                     QSRQQAAQVADLKQQLGDSDQLQRDIQRQQAELAHLRKQQEAAQQARDEYARRQGEIA
                     QLRQQEQDNARAIADAHRARMEEAQLRDQERRQKDELARAQKEQVTQKRRGIDTVVTV
                     KRPAPLPADDKPATVKASGRGTTMTRRSHKHRSTRSASSQATPPSYHKPSAEEAEDIA
                     RLEASPLVAMHSGAVAAEPAAAARNRQSASDVACQANPKGCIR"
     gene            complement(3003..3317)
                     /locus_tag="VHE37_11175"
     CDS             complement(3003..3317)
                     /locus_tag="VHE37_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36142.1"
                     /translation="MSRTCLLTVLLLCAFGAPAVRAQDQTSGDDARPMTQRMCERSSG
                     SCDDMALRHEQCQKFPQACPGVTPPGPQPRSAAASTGTGYRPPSHTRGPHCGTRGHHC
                     PR"
     gene            complement(3418..3768)
                     /locus_tag="VHE37_11180"
     CDS             complement(3418..3768)
                     /locus_tag="VHE37_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36143.1"
                     /translation="MKHRYLLPLLCFTAALTLAPAYAQDAATTGSAPSGRGAEFCKNN
                     PATCQKMQERKKECDANPQQCEQLKQERQARMAKMKQACDANPEQCQKKKDAWRQRMD
                     QRAAQRQQPPAPAQ"
BASE COUNT          490 a         1272 c         1398 g          721 t
ORIGIN      
        1 actcgtaggg gaagccgctc tccaggtgca gctcgccgcc gtcggtcatc acctcgtcgg
       61 tcggttcgct tgatccggtg tggtacatgc gcggtgcgtc gccggcttcc tgcaggttca
      121 tgtggaagtc gaccaggttc atcacgatct gcgcgtggcc ctgcggctgc atgtcgccgc
      181 ccatcacgcc gaagctcacc cagggtttgc cgtccttggt gatgaaggcg gggatgatgg
      241 tctggaacgg ccgcttgccc ggcgcgtaca cgttcacgtg tccgggagtg agcgagtaca
      301 tctcgccgcg gtcctgcagt acgaaaccca ggcccggcgg cgtcatgccg ctgcccatgc
      361 cgcggtagtt ggactggatc agcgagacca tcatgccgct gctgtcggcg gtggtcaggt
      421 acacggtgtc gccctgtttc atcgccgggt tgccggcgtc gtagcgtcgc gcggcgtgat
      481 tcatatcgag cagcttgcgg cgcccgtcgg cgtatttctt gtcgatcagc cccgcaaccg
      541 gcagcgcgtt gaacgcgggg tcggcgtagt acttggcgcg atcctcgaac gccagcttct
      601 tggcctcgac gtaggcgtgc atgtggtcag cgctgccgaa accgaatttg ccgatgtcga
      661 aaccttccag gacgttgagg atctgcagcg ccgcgatgcc ctgcgagttc ggcggcagtt
      721 cccacacgtc gtagccgcgg tagctggtgc tgaccggctc gacccaatcg ctgtggtgcg
      781 cggcgaagtc cccgtacgag aggaatccgc cctgctggtg gatgtactgg tcgatcacgt
      841 gcgcgatgtc gcccttgtag aacgcatcgc gcccgccgcg cgcgatggtc tcgagcgtgt
      901 gcgcgaggtt cgggttcttc cagatctcgc ccttgcgcgg cgcgcggccg ttgatggtca
      961 tctgctcggc gaacccgggg aacttcgaca gccgcggcac cgacttgtcc cagtagaacg
     1021 cgatcagttc ggacagcggg aagccgttgc gcgcgtagcc gatcgcgggc gccaggtcgt
     1081 ccttcatcgg cagcctgccg aatttgccgt gcagctcgaa ccagccgtcg acgcagcccg
     1141 gcactgtcac cggcagcggg cccagcggcg ggatgtgctt gaggccctgt tgctggaaat
     1201 attccagcgt gagcgatttc ggcgagcggc cgctggcatt gagcccgtag agcttctgcg
     1261 tcttcgggtc ccacacgatg gcgaacagat cgccgccgat gccggcgccc accggttcca
     1321 tcagcccgag cgcggcgtcg gcggcgatgg ccgcgtccac cgcgctgccg ccgcgcttga
     1381 gcacgtcgag cgcgatctgc gtggccagcg gctggctggt ggccgccatg ccgtgctggc
     1441 cgatcacctc ggcgcgggtg gcgaaggaca gcccggtgac gcggtcgggt ccggcggaag
     1501 ccgcaatgga agtcatggcg agggcgagcg cgagggtttt catcgcgcga atgtagcgcg
     1561 ccggccatca cggaaaaagg ccgcgcggcg gcgcggcctg cggtcagcac tccgagtcag
     1621 cggatgcagc ctttcggatt ggcctggcag gcaacgtcgc tggcgctctg ccggttccgc
     1681 gccgccgccg cgggctctgc cgcgaccgca ccggaatgca tcgcgacgag cgggctcgct
     1741 tcgagccgcg cgatgtcttc cgcttcctcc gccgatggct tgtgataaga cggtggcgtg
     1801 gcctgcgacg acgccgagcg ggtggatctg tgcttgtgcg agcggcgcgt catggtggtg
     1861 ccgcggccgc tggccttcac cgttgccggt ttgtcgtcag cgggcagcgg agccgggcgc
     1921 ttgaccgtca ccaccgtatc gatgccgcgg cgtttctgcg tcacctgttc cttctgcgcc
     1981 cgcgccagct cgtccttctg ccggcgctcc tggtcgcgca gctgtgcttc ctccatgcgc
     2041 gcgcgatggg cgtcggcaat ggcgcgcgca ttgtcctgct cctgctggcg cagctgcgcg
     2101 atctcgccct ggcgccgcgc atattcgtca cgcgcctgct gcgccgcttc ctgctgcttg
     2161 cgcagatggg ccagctcggc ctgctgccgc tggatgtcgc gctgcagctg atcggaatcg
     2221 cccagctgct gcttgaggtc ggccacctgc gcggcctgct ggcgcgactg ttcgcgcagc
     2281 tgcgtcacct gctgctgccg ggcctgcagg cgggcgagtt cctgctgctg ctggtcgatc
     2341 tgcgcctgca gccggtcgct gcgcgccttc agccgctgct ctgtcgcatt cgggcgtgcg
     2401 gcagttgccg cggcgggcgg cggcgctgcc ggtgcggcga ctgcggtgtc ctcgcgctcg
     2461 atggattcca ggtgcggctg ctcgggttca tcggccggtg caggatgctc gacagcagga
     2521 tgctcgacgg cgatgaccgg cgccggcgga gcagccgcgg cagcctcggc cggcggtgac
     2581 ggtggtgcct gcggttgcgg tgccggttgc ggcggagcaa cgcgggcctg caccgcgcac
     2641 ccggcatcgc cttcgcggca tgccggtgcg agcagggctg gcggcggcga cgaggcgggc
     2701 ggcgcggaca ccggccgcgg gctgccgctg gcatgttcgt cgcaggtctc ggggtatttc
     2761 ttgcagcggt aatagcggac ctcggcaggt gtgcagccgc cggggaacgt gctgcagatg
     2821 aattcgtcac cgccggccgc cgcggcgggt ggatcgccgg cgcgcgcggc cgtcaccatc
     2881 aatggcgcca gcaaggccgt catgatgaaa gttctggcgg tcatggcttc ctcttcagtc
     2941 cgggcttttt ccgtttcgac tcgccatgtc ggcattggtg cgaaaaatct ttgccgctga
     3001 actcagcgcg ggcagtgatg tccgcgcgtg ccgcaatgcg ggccgcgcgt gtgcgacggc
     3061 ggacggtaac cagtgccggt ggaggccgcc gccgagcggg gctgcggccc cggtggagtg
     3121 acgcccgggc aggcctgcgg aaatttctgg cattgctcgt ggcgcagcgc catatcgtcg
     3181 cagctgccgg acgagcgctc gcacatgcgt tgcgtcatcg gccgcgcgtc gtcgcccgag
     3241 gtctggtcct gggcccgcac cgccggggcg ccgaaagcgc acagcagcag cacggtcagc
     3301 aggcaggtac gggacatcgc aggctccggc attcacaacg ttggacctgc gtgatgcgcc
     3361 gccggtgcgg caaaacgcga aaagcccctt gcggggcttt ccggtgatgc ggtgaaacta
     3421 ttgcgcgggc gccggcggct gttgccgctg cgcggcgcgc tggtccatgc gctggcgcca
     3481 ggcgtccttc ttcttctggc attgctccgg gttggcgtcg caggcctgct tcatcttggc
     3541 catgcgcgcc tggcgttcct gcttgagctg ttcgcactgc tgcgggttgg cgtcgcattc
     3601 cttcttgcgc tcctgcatct tctggcaggt ggcgggattg ttcttgcaga actcggcgcc
     3661 gcggccggag ggagcgctgc ccgtggtggc ggcgtcctgg gcataggcgg gcgccagcgt
     3721 cagcgcggcg gtgaagcaca gcagcggcag caggtaacgg tgcttcatgg gaactccggc
     3781 ggggatcggg gagcctaatc tagctccgtt gcgtgaacct ctgctgaagc tgcctacgac
     3841 gagcaactga cctcgatgcg ccgcgcgtcc ggcggcggct c
//
LOCUS       DASZZC010000526         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_365629,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000526 DASZZC010000000
VERSION     DASZZC010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365629"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VHE37_11185"
     CDS             <1..600
                     /locus_tag="VHE37_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36144.1"
                     /translation="DTLPVAAGMNRFVWNLRYDDPVNIPDAFYSGLAPRGPIVAPGHY
                     TLRLTVDGQALTAPLTVARDPRMHGPDDALMAKFALSMKVYHDQDALHRAVLDIRGAR
                     ETLKNAASADAQALAARASSIEGRLMQVQIHSSEGSLLFPVLLNEQIYALAWMLEDAD
                     TAPTREMVQAFDAAHAQLTALLAEWDEVKGRIASAVPAK"
     gene            complement(1012..1833)
                     /locus_tag="VHE37_11190"
     CDS             complement(1012..1833)
                     /locus_tag="VHE37_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HVT36145.1"
                     /translation="MPLYIDIHELKGVRAADVAKAHAADVEKQGAHGVNYLKYWVNES
                     CGKVFCLVDAPSSEAAVCVHREAHGLEPGRIIEVQPEVVDAFVGGGEVNEVGAALVPG
                     RSELDTAMRTILFTDMVDSTSMTQQLGDRAALRLLELHDTVVRDCLARLHGREVKHTG
                     DGIMACFLFAPDAVQCGLQIQRELAARSSELDHAVRLRIGAAAGEPVEQRGDFFGLTV
                     NLAARLCAHAQPEQVLVSTDVVDLCSSADLRFEGIGELTLKGFDRPVAAHAAMWE"
     gene            complement(1888..>2457)
                     /locus_tag="VHE37_11195"
     CDS             complement(1888..>2457)
                     /locus_tag="VHE37_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVT36146.1"
                     /translation="GAVQATMIGHALRRGERLRGPQLAGFALALGGLVALLAPGLSAP
                     PLAGSLLMLLAGVAWGIYSLRGKNAGDPTRVTAGNFLRACAFALALSALAHGSAAFDG
                     AGFCYAVLSGALASGVGYAIWYSALPALTATAAATVQLSVPVIAAIGAVMFLGESVTL
                     RLLLASIAILGGIALVVLNKPAARAEPRQ"
BASE COUNT          382 a          860 c          852 g          363 t
ORIGIN      
        1 gacacgctgc cggtggccgc cggcatgaac cgcttcgtct ggaacctgcg ctacgacgac
       61 ccggtgaaca tccccgacgc cttctactcg ggcctggcgc cgcgcgggcc gatcgtcgcg
      121 cccggccact acacgctgcg cctcaccgtc gacggccagg cgctgaccgc gccgctgacg
      181 gtggcgcgcg acccgcgcat gcacgggccc gacgacgctc tgatggcgaa gttcgcgcta
      241 tcgatgaagg tctaccacga ccaggacgcg ctgcatcgcg cggtgctcga catccgcggg
      301 gcgcgcgaga ccctgaagaa cgccgccagc gccgacgccc aggcgctcgc agcgcgcgcc
      361 agcagcatcg aagggcgcct gatgcaggtg cagatccatt cctcggaagg ctcgctgctg
      421 ttcccggtgc tgctcaacga gcagatctac gcgctggcct ggatgctcga ggacgccgac
      481 accgcaccga cgcgcgagat ggtgcaggcc ttcgacgcgg cgcacgcgca gctgacggcg
      541 ctgttggcgg agtgggacga ggtaaaggga agaatcgctt ccgcggttcc ggcgaagtag
      601 tcgccgcgcc tggaccgcca ggggtcggaa cgggcaatcg gcactgagtc gagcgccggg
      661 tagattcact gcccggcgcc tttgctatcg tctgaagcgg gccgactcag tcgtcgtaac
      721 ccggctcctc gactgcgcca tccgcgcagt tcgcgctgag ggtcggtgat gcgtcaggca
      781 ttttccagca tcgaagagct cgccgcggcc atgcagtcac ttggtgtgcc gggcgcagcg
      841 gcacggcaag ttggagcggc atcggaacag agtgcacatc ccgccgcgtt ggcgcgactt
      901 ccagtgctcg cttcgcggcg gcgagtattg agggacgatt ggcggcagtg ggtcggtcag
      961 agaccttggt ccagactttt ctggcgaccg ttcccggcag cctccgggca tctactccca
     1021 catcgccgca tgcgccgcca ccggccggtc gaatcccttc agggtgagtt cgccgatgcc
     1081 ctcgaagcgc agatcggcgc tgctgcacag atcgaccacg tcggtggaaa ccagcacctg
     1141 ttccggctgc gcgtgcgcgc acaggcgcgc ggcgagattc accgtgaggc cgaagaagtc
     1201 gccgcgctgt tccaccggct cgccggccgc ggcgccgatg cgcaggcgca cggcatgatc
     1261 gagttcggac gagcgcgccg cgagttcgcg ctggatctgc agtccgcact gcacggcatc
     1321 gggcgcgaac aggaagcagg ccatgatgcc gtcgccggtg tgcttcacct cgcggccgtg
     1381 gagccgcgcc aggcagtcgc gcaccacggt gtcgtgaagc tccagcaggc gcagcgccgc
     1441 gcggtcgccc agctgctgcg tcatgctggt ggagtcgacc atgtcggtga acaggatcgt
     1501 gcgcatggcg gtgtcgagtt cgctgcggcc cggcaccagc gcggcgccca cttcgttgac
     1561 ctcgccgccg ccgacgaagg cgtcgaccac ttccggctgc acttcgatga tgcggcccgg
     1621 ttcgagtcca tgcgcttcgc ggtgcacgca gaccgcggcc tcggagctgg gcgcgtcgac
     1681 caggcagaag accttgccgc agctttcgtt gacccagtac ttcaggtagt tgacgccatg
     1741 cgcgccctgt ttctcgacgt cggccgcatg ggccttggcc acgtcggcgg cgcgcacgcc
     1801 cttgagctcg tggatatcga tgtacagagg catggccggc tccatccgca cgagtgaggc
     1861 ggcagtgtag cccagccgcg tgcggcgtca ttgccgcggt tcagcgcgcg ctgccggctt
     1921 gttcagcacc accagcgcaa tgccgccgag gatggcgatg gacgccagca gcaggcgcag
     1981 cgtgacgctc tcgccgagga acatgacggc gccgatcgcc gcgatcaccg gcacgctcag
     2041 ctgcacggtc gcggctgcgg tggccgtcag cgcgggcagc gcgctgtacc agatggcgta
     2101 accgacgccg gacgccagcg cgcccgacag caccgcgtag cagaagccgg cgccgtcgaa
     2161 ggctgcgctg ccgtgcgcca gcgcgctcaa cgcgagcgcg aaggcgcagg cgcgcaggaa
     2221 gttgcccgcc gtcacccgcg tgggatcgcc ggcatttttt ccgcgcagcg agtagatgcc
     2281 ccaggccacg ccggctagca gcatcagcag cgatcccgcc agcggcggcg ccgacaggcc
     2341 cggcgccagc agcgccacca gtccgccgag tgccagggca aatccagcca gttgcggtcc
     2401 gcgcagccgt tctccgcggc gcagcgcgtg gccgatcatg gtggcctgca cggcgcc
//
LOCUS       DASZZC010000527         5703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_417790,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000527 DASZZC010000000
VERSION     DASZZC010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417790"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VHE37_11200"
     CDS             complement(<1..371)
                     /locus_tag="VHE37_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36147.1"
                     /translation="MDGHIPLNTGGRRAESLAVNPTGNEYAFDDYDTQRFVPVASAFG
                     QSRYGFVVTPNVDHLVRLQKEPRFAYAYNVASYVLLDSRLLARLLRWRRGIELPVCPG
                     SELTEALLRDGVHPDDRIVLIG"
     gene            complement(395..1588)
                     /locus_tag="VHE37_11205"
     CDS             complement(395..1588)
                     /locus_tag="VHE37_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36148.1"
                     /translation="MFCLLLCAYAHPAAAQFKFDASAAALEQYDSNVFRLSSSGQAYD
                     PVSAKFDDKRDDSILNGVATARLEYKFDDQRIYLNGDGTRQWYHHYKVLDHDEYNAGG
                     GFDWRATDWVDGSASAHQDHRMVPQSARLLPTLTVAMQKDQNASGGINFTAGKLWRLE
                     TRFDWQKQDAPSPDAQFFMNERDYTVAGKYLGLGPAAVGLQVQYIDGVFTAPVLPPNT
                     VVTPANYRTFALGTSPYHQYNEDLTVDYTVSSLSSFTTKLGVSQRRDPQGVQPPTNGF
                     TGELQYKRTISPLTTLTFALQRKLQSYPSFNDFVVESGGYGQLEWRPTLKIYTNLLAQ
                     YLEDSFHSADRADRLALVDAGVAYQVFEHFSIKPGFHYEDRRSSGGVYSYVDRIYSME
                     AKLIF"
     gene            complement(1659..3101)
                     /locus_tag="VHE37_11210"
     CDS             complement(1659..3101)
                     /locus_tag="VHE37_11210"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HVT36149.1"
                     /translation="MDGHAAVPSVKGEAARFGTVVDYATPGASSRRVQGLVYTAVISG
                     LLPLIVQASGANFTVAYQVLALMVGISSYFSLRHLSLTAVWDTGRRGSAGTELLLLWT
                     KIAVIFLLLGIVTGYYRYYEPKIIVTWFVTAPLALVMLNNQLRKLIRSSELVAGDTRT
                     AVLVFANDTARQFAQRLQASHSHELLGYFEDRDLERAGHVDGVAYLGTSRDIADYVKQ
                     HGVDVVFIILPIVGSERALAIAESLGDTTASVFFVPDYCAFRNLGARATEVNSMPALE
                     LMETPFYGADGLLKRAFDIAFSLFALAVLALPMAIIAALVKLTSPGPALFKQQRYGLN
                     GKSFDVYKFRTMYVANRDQEIVQVSRTDTRVTPLGRILRRTSLDELPQFINVLLGDMS
                     VVGPRPHTVVHNEHYRRQVRRYMSRHKVRPGITGLAQVRGLRGETTTLALMDARINCD
                     IEYIRTWSVLLDLKIILRTILVVLNDKHAY"
     gene            complement(3280..4566)
                     /locus_tag="VHE37_11215"
     CDS             complement(3280..4566)
                     /locus_tag="VHE37_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36150.1"
                     /translation="MKPLPRLEGALVKLLGSALIDQALLSACNFLVSVLLIRMAADHQ
                     YGYYVLATNAVLLLVTLQNAFFGAPLVNALTRRPLAEHAPLVEGLNHDQQRYLLLAVA
                     LCLAVAAAGWISGLLAGETALVLAAFGLLMLCMLTREFFRAVLIARRCTHDVLRADAM
                     YVAVLVAGVCIAARGPAPAAAALVAMSLAALAGVAILRRAARSASLLGAAGTPGTLRR
                     LAPLGAWAAAGGAIHWTFSQGYSYLVAGTLDLAAVAAIAAIRLLMMPLNLLSTGMYQL
                     MMPTVSRWLHDHGPQLVLKRLLLVAAAFMAVAALYFVVVWLLREWIFGSLLKKQFEQR
                     DLLLCLWAGIFFLMVMRDQLIFLPVLAERFRELAGLALACAVLALSVSFIAVQRHGAP
                     GALLGMLVGEAADLAGILYLGWRETRLPRPAPLLAA"
     gene            complement(4563..>5703)
                     /locus_tag="VHE37_11220"
     CDS             complement(4563..>5703)
                     /locus_tag="VHE37_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT36151.1"
                     /translation="AAASLLLSLAWAYLLLLTLCSRQPGRSPPGGRQLKFAIVVPAHN
                     EAGGIAATVADLRALDWPRRQFSVIVVADNCTDDTATQAAAAGARVLQRNDPSRRGKG
                     YALEFAFAQLLGEAWADAVVVIDADTRADAHLLASLASAITGGAQVVQAHYAVLNPDA
                     SWRTRLMTIAMAAFHAVRSRGREFLNLSCGIRGNGWCLTRQVMQAVPYRAYGLVEDVE
                     YGGRLALDGYRVAYLEAACVRAAMVSRAEHAASQRRRWELGRRQLRRSMAPALLGAAF
                     RPHGRVCLDLAADLLVPPLTQLVLLTAAATAVALYLHIGSAAWLGATDFVALVLYVGR
                     GWHLSGLGWRCAADFLRAPWFMLWKLAVLLKPHSGLWTRTQREHT"
BASE COUNT         1016 a         1828 c         1944 g          915 t
ORIGIN      
        1 ccgatcagca cgatgcggtc gtcgggatgc acgccgtcgc gcagcaacgc ttcggtgagt
       61 tcgctgccgg ggcacaccgg cagctcgatg ccgcggcgcc agcgcagcag cctcgccagc
      121 aggcggctgt cgagcagcac gtaggaggcg acgttgtagg cgtaggcgaa gcgcggttcc
      181 ttctgcagcc gcaccaggtg atccacgttc ggcgtcacca cgaagccgta gcgcgactgg
      241 ccgaaggcgc tggccaccgg cacgaagcgc tgagtgtcgt agtcgtcgaa ggcgtactcg
      301 ttgccggtgg gattcaccgc cagcgattct gcccggcgcc cgccggtgtt gaggggaata
      361 tggccatcca tggctgcctc cggggtgttg cgcgtcagaa gatcagcttc gcttccatgc
      421 tgtaaatccg gtcgacgtag ctgtaaacgc cgcccgacga gcgccggtct tcgtagtgga
      481 agcccggctt gatggaaaaa tgctcgaaca cctggtaggc gacgccggcg tcgaccagcg
      541 ccaggcgatc ggcgcggtcc gcgctgtgga agctgtcttc caggtactgg gccagcaggt
      601 tggtgtagat cttcagcgtc ggccgccact ccagctggcc gtagccgccg ctctccacca
      661 cgaagtcgtt gaagctgggg tagctctgca gcttgcgctg caaggcgaag gtgagcgtgg
      721 tcagcgggct gatcgtgcgc ttgtactgca gctcgccggt gaagccgttg gtcggcggtt
      781 gcacgccctg cggatcgcgc cgctgcgaca cgccgagctt ggtggtgaag ctggacagac
      841 tgctcaccgt gtagtcgacg gtgagatcct cgttgtactg gtgatacggg ctggtgccaa
      901 gcgcaaacgt gcggtagttg gctggtgtga ccacggtgtt gggcggcagc accggcgcag
      961 tgaagacgcc gtcgatgtac tgcacctgca ggcccaccgc ggccggcccc aggcccaggt
     1021 acttgccggc cacggtgtag tcgcgctcgt tcatgaagaa ctgcgcatcg ggcgatggcg
     1081 catcctgctt ctgccagtcg aaacgcgttt ccagccgcca cagcttgccg gcggtgaagt
     1141 tgatgccgcc ggatgcgttc tggtctttct gcatggcgac ggtgagcgtc ggcagcaggc
     1201 gcgccgattg cggaaccatg cggtggtcct ggtgggcgct ggcgctgccg tcgacccagt
     1261 cggtagcgcg ccagtcgaag ccgccgccgg cgttgtactc gtcgtggtcc agcaccttgt
     1321 agtggtgata ccactgccgg gtgccgtcgc cgttcaggta gatgcgctga tcgtcgaact
     1381 tgtattccag gcgtgccgtg gccacgccgt tgaggatgct gtcgtcgcgc ttgtcgtcga
     1441 acttggcgct gaccggatcg taggcctggc cgctggaaga caggcggaac acgttggagt
     1501 cgtactgttc cagcgcggca gcgctggcgt cgaacttgaa ttgcgcggct gcggggtgcg
     1561 cataggcgca cagcagcagg caaaacactg ccgcccgcat cgatgatgcg tgcggcggtt
     1621 caatcggacg aaatggggaa aggggcaggc gtccgcaatc aatacgcgtg cttgtcattg
     1681 agcaccacca ggatggttcg aagaattatt ttcagatcca gcagtaccga ccaggttcgg
     1741 atgtattcga tgtcgcagtt gatgcgcgca tccatcagcg ccagcgtggt ggtctccccg
     1801 cgcaggccgc gcacctgtgc cagcccggtg atgcccgggc gcaccttgtg ccggctcatg
     1861 tagcggcgca cctgccggcg gtaatgctcg ttgtgcacca ccgtgtgcgg gcgcgggccc
     1921 accacgctca tgtcgcccag cagcacgttg atgaattgcg gcagctcatc gagcgaagtg
     1981 cggcgcagga tgcggcccag cggcgtcacc cgcgtatcag tacgcgacac ctgcacgatc
     2041 tcctgatcgc ggttggccac atacatggtg cggaacttgt agacgtcgaa cgacttgccg
     2101 ttgaggccgt agcgctgctg cttgaacaac gccggacctg gcgaggtgag cttgaccagt
     2161 gcggcgatga tcgccatcgg aagcgccagc acggccagcg cgaacagaga gaaggcgatg
     2221 tcgaaggcgc gcttgagcag gccgtccgct ccgtagaacg gcgtttccat cagctccagc
     2281 gccggcatcg aattcacttc ggtggcgcgc gcccccaggt tgcggaaggc gcagtagtcc
     2341 ggcacgaaga ataccgaggc ggtggtatca cccaggcttt cagcgatggc gagcgcccgc
     2401 tcgctgccga cgatgggcag gatgatgaag acgacgtcca cgccgtgctg cttcacgtag
     2461 tcagcgatgt cgcgcgacgt tcccaggtag gccacgccgt cgacatgccc ggcgcgttcc
     2521 aggtcgcggt cctcgaaata tccgagcaat tcgtgcgaat gcgatgcctg caggcgctgg
     2581 gcgaactggc gtgcggtatc attggcgaac accagcacgg cggtgcgcgt gtcgccggca
     2641 accagttcgc tgctgcggat cagcttgcgc aactggttgt tcagcatcac cagggccagc
     2701 ggcgcggtga cgaaccaggt gacgatgatt ttcggctcgt agtaacggta ataaccggta
     2761 acgatgccga gcagcaggaa gatcaccgcg atcttggtcc acagcagcag cagctctgtc
     2821 ccggcggagc cgcggcggcc tgtatcccag accgcggtga gtgagaggtg gcgcagcgag
     2881 aaatagctgc tgatgcccac catcagggcc agcacctggt acgccaccgt gaagtttgcg
     2941 ccgctggcct gaacgatcag ggggagcagg ccgctgatga ccgcggtgta gaccagacct
     3001 tgcacgcggc gggaggatgc tcccggcgtc gcgtaatcca ccacagtccc gaacctggct
     3061 gcttcgccct tgaccgaggg gaccgctgcg tgtccgtcca tcttgccgcg ctcctgtgtt
     3121 acgtgtcggc ctggtgggcc ttgcgttcgt cggattggag cgcgcctgca ggaaaaaatt
     3181 cagttatttg aaattagaag gttttaccgg cgcagagtta ttttttcacc ccgatttaca
     3241 cagttatctc gacctcaaca gggtggcgct cagcagttgt catgccgcca gcagcggcgc
     3301 agggcgtggc aggcgcgtct cgcgccagcc caggtagaga atgcccgcga gatccgctgc
     3361 ctctcccacc agcattccca ggagcgcgcc gggagcgccg tgacgctgca ccgcgatgaa
     3421 gctcacggac agggccagca ccgcgcacgc gagggcgagg ccggcgagtt cgcggaagcg
     3481 ttctgccagc accggcagga agatgagctg gtcgcgcatc accatcagga aaaatatccc
     3541 ggcccacagg cacagcagca ggtcgcgctg ctcgaactgc ttcttcagca gtgaaccgaa
     3601 gatccactcg cgcagcagcc agacgacgac gaagtaaagg gcggcaaccg ccatgaaagc
     3661 ggccgcgacc agcaacagcc tcttgagcac cagttgcgga ccatggtcgt gcagccagcg
     3721 cgacacggtc ggcatcatca actggtacat gccggtggac agcaggttca gcggcatcat
     3781 cagcaggcgg atcgccgcga tggcggcaac cgcagcgagg tcgagcgtgc cggcgaccag
     3841 gtagctgtag ccctggctga aggtccagtg gatcgcgccg cccgcagcgg cccacgcacc
     3901 cagcggcgcc aggcggcgca gcgtgccggg agtgcctgca gcccccagca ggctcgcgct
     3961 gcgggctgcc cttcgcagga tggcgacgcc ggccagcgcc gcgagcgaca tcgccaccag
     4021 cgcagctgcc gccggtgcag ggccgcgtgc cgcaatgcag acgccggcca ccagcacggc
     4081 cacgtacatg gcgtcggcac gcaacacgtc gtgggtgcag cggcgcgcga tcagcacggc
     4141 gcggaagaat tcgcgcgtga gcatgcacag catcagcagg ccaaacgcgg ccagcaccag
     4201 cgcggtttct ccagccagca agccgctgat ccagccggcc gccgcaaccg ccaggcacag
     4261 ggccaccgcg agcagcaggt agcgttgctg gtcgtggttg aggccctcca ccagcggggc
     4321 gtgttccgcc agcggccgcc gcgtaagggc attcacgagc ggcgcaccga aaaaggcgtt
     4381 ctgcaatgtc accagcagca gcacggcatt ggtggccagc acgtagtagc cgtactggtg
     4441 atcggcggcc atgcggatca gcagtacgct gaccaggaag ttgcaggcag agagcagtgc
     4501 ctggtcgatg agtgcgctgc ccagcagctt gaccagcgcg ccttccagcc gcggcaatgg
     4561 cttcacgtgt gttcgcgctg ggtgcgcgtc cacaggccgc tgtgcggttt gagcagcacg
     4621 gcgagcttcc acagcatgaa ccacggcgcc cgcaggaagt cggcggcgca gcgccagccc
     4681 aggccactga ggtgccagcc gcggccgaca taaaggacca gcgctacgaa atccgtagcg
     4741 ccgagccagg ccgccgagcc gatgtgcagg tacagcgcga cggcggtcgc cgcggcggtc
     4801 agcagcacca gttgagtcag cggcgggacc agcagatctg ccgccaggtc caggcacacg
     4861 cgaccgtgtg gccggaaagc ggcgcccagc agcgcaggtg ccatcgagcg acgcagttgg
     4921 cgcctgccca gttcccagcg acgccgctgc gacgccgcat gttctgcgcg actgaccatg
     4981 gcggcgcgca cgcaggccgc ttccaggtag gcgacgcggt atccgtccag cgccagccgt
     5041 ccgccgtatt ccacgtcttc caccagccca taggcccggt atggcaccgc ctgcatcacc
     5101 tggcgggtga ggcaccagcc attgccgcgg atgccgcagg acaggttcaa aaattcgcgg
     5161 ccgcgcgagc gcacggcgtg aaaggccgcc atggcgatgg tcatcagccg cgtgcgccac
     5221 gatgcgtcag gattgagcac ggcatagtgc gcctgtacca cctgtgcgcc gccggtgatg
     5281 gcggacgcga gcgatgccag caggtgcgca tcggcgcgtg tgtcagcgtc gatcaccacg
     5341 accgcgtcgg cccacgcttc accgagcagc tgcgcgaagg cgaattccag cgcgtaccct
     5401 ttgccgcgcc gcgaaggatc attccgttgc agcacgcgcg cgcctgcggc ggccgcctgc
     5461 gtcgcggtgt cgtccgtgca gttgtcggca acgacgatga ccgagaactg gcggcgcggc
     5521 cagtccagcg cccgcaggtc cgcgacggtg gcggcgatcc cgcccgcctc gttgtgcgcc
     5581 gggaccacaa tggcgaactt gagctgccgg cctcctggag ggcttcggcc tggttgccgc
     5641 gaacacaagg tcagcaacag aaggtaggcc caggccaggc tgagcaacag gctggcggcg
     5701 gca
//
LOCUS       DASZZC010000528         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_417846,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000528 DASZZC010000000
VERSION     DASZZC010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417846"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..847
                     /locus_tag="VHE37_11225"
     CDS             113..847
                     /locus_tag="VHE37_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT36152.1"
                     /translation="MDASAAVSSVARRAAAKGPRVGRPPTIHVDDIIAAALELGLEGV
                     TLKQVADHLGVAIATLYRHVAGRDELVRLAAFRLTLQRGLPEARAAHWSELATRYAQT
                     LYASFLAEPQLIGELLRGRIGPHAEVDVLEQFIAAVHGHGFSEAESAQLFHAIGMVTI
                     GAAAGAIGKKAADESGAPWKRAVQRTLAERDAHELKRVRRVLPAALELDPIPWLPTLQ
                     RLLEGIAAARGEKLPKPTSGKTRRAT"
     gene            844..2127
                     /locus_tag="VHE37_11230"
     CDS             844..2127
                     /locus_tag="VHE37_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVT36153.1"
                     /translation="MSNDTASAGRIEIDGRKFNPYSKDFIRNPHDTWQMLIRDYPIAW
                     HKDLQMWLVNSHELNDRMLKDNRFSPNFREWKLAPPLKADADKNHFDLALEKGFFAVD
                     QKAHLRLRKLTMPAFSKPVMGKIDAKIRDLVTQCFDAIGDAEEFDVYQAIASKLPVRA
                     IARMIGVAVDNEDFFHDFAVNVIKGTRINLPIKERNAAIENSLPGFQFFLDQIKARRA
                     QATPGDDFLGSLVGSVVDGERLDDYEILSVVFALITAGSDTATDLHTFAIDGLLRNPD
                     QYELLKNKPEIMENAIIELLRYGSFGKMPFFRFATGDYEFAGQHIAKGEAIGVNVSAA
                     WNDPAKFPDWNKLDLQRRLDGNLVFGAGAHFCIGTYLVRVQGGLMLTEFMKRFPNATL
                     ADGNGHVDYEYTHHNARRINKLVVRTNRAKMQKAA"
     gene            2127..2291
                     /locus_tag="VHE37_11235"
     CDS             2127..2291
                     /locus_tag="VHE37_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HVT36154.1"
                     /translation="MDKYICVICGFIYDPAVGLPQHGIAAGTRFEDLPADWICPECGS
                     PKESFEIYTG"
     gene            2504..3874
                     /locus_tag="VHE37_11240"
     CDS             2504..3874
                     /locus_tag="VHE37_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT36155.1"
                     /translation="MKLKLVLTSCVAAVTFCAAQATLAKVPPEKAAELDGPKYTCMGA
                     ERAGNGKDIPEYTGKFQGSWPGMKGKSGFEPGPYADEKPLFTISAANMAQYADRLTEG
                     EKALMKKYPDNYKMNVYPSHRDFAEPKWACDATKKNVGTSEIIHNGLGITGVAGGVAF
                     PFPQSGLEAIWNAINPPRVYNEEAICDVADVYANGSIAWGRNHFKVLALTFDPNHRGS
                     FQDKVNSYFYTSYLLPERDKGFTAVGYQPNDFTDDSTQSWQYLPGLRRVRQAPEVGFD
                     YPVPPAGLRTVDDDYGFNGSPERYTWKLVGKKEMYVPYDNFKVNDPAVKYKDLIKPGS
                     VNPEYERYEAHRVWVLEGTLKPGVRHIYSKRLLYVDEDSWLTLMADNYDARGQLWRVA
                     LVNFHYSQESGTWHRGATIYHDLTSGAYEAGYLTNEAGEHWWKINQPGMTDAQFKPEA
                     AARGGH"
     gene            3934..4797
                     /locus_tag="VHE37_11245"
     CDS             3934..4797
                     /locus_tag="VHE37_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36156.1"
                     /translation="MKTAFVTGANVGQANLLVKALAQRGWRVFAGVLPGAPTDLKSEG
                     NITVVDQDVSKTDSVKASAEVVTKALNGGGLDLLMNVAGVANIAVGVVEGADLAQAER
                     LFQINLFGQLRVIQSFLPLVRRNAPGSRIANYSSGAILANPIGAGIYNMTKHAIHGMT
                     LTLRHELAPLGIQVTSIIPGGVLTGMSANSHQNTRNSWNAQREDLRQVYQPLENVMCQ
                     VLPDMLEKSGSTPEQALAGTLEILDKKKWKPMEYLGKDVKPMGIMRRILSDSQLESMM
                     RGAFKIPAYKA"
     gene            4808..>5453
                     /locus_tag="VHE37_11250"
     CDS             4808..>5453
                     /locus_tag="VHE37_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVT36157.1"
                     /translation="MNAVLKESPSDISAIFTGLAAKAPELAQTSAAQRADKLRRLLAA
                     VMAARPAILAAGKKELRLVDLDIDAQLMMVKGEIEFACKHLAQWMAPEDIQPTMMTLG
                     KRSYVKYEPKGVVLNLATWNAPIAIAFVPLVGAIAAGNAVLLKPSELAPHSAQVLADI
                     VATVFPPDEFAVVQGGPEVAQEVLRQPFNHIYYIGGHAVGRLVMKAAAEHFASVT"
BASE COUNT         1071 a         1783 c         1759 g          840 t
ORIGIN      
        1 gaatcgcgcg caaggcgcgc tcctgccggc aattgcggtc atccatctgg acgattgctt
       61 tacgagaatc aatttgtgaa actgattctc gtaattgtgg gggcaagacc ggatggacgc
      121 atcggcagca gtatccagcg tggcgcgccg tgctgcggcg aagggcccgc gcgtcggccg
      181 tccgccgacc atccacgtcg acgacatcat cgccgcggcg ctggagctgg ggctggaagg
      241 cgtcacgctc aagcaggtgg ccgatcacct gggcgtggcc atcgccacgc tgtaccggca
      301 tgtcgcgggc cgcgacgaac tggtgcggct ggccgcgttc cgcctcacgc tgcagcgcgg
      361 cctgccggag gcgcgggcgg cgcactggtc ggaactggcc acgcgctacg cgcagacgct
      421 gtatgcatcg ttcctggccg agccgcagct gatcggcgaa ttgctcaggg gccgcatcgg
      481 cccgcacgcc gaggtcgacg tgctggagca gttcatcgcc gccgtgcatg gccatggatt
      541 ttccgaggcg gagagcgccc agctgttcca cgccatcggc atggtcacca tcggcgcggc
      601 ggccggcgcc atcggcaaga aggcggccga cgagtccggc gcgccgtgga agcgcgcggt
      661 gcaacgcacg ctggccgagc gcgacgcgca tgaactgaaa cgcgtgcgcc gggtgctgcc
      721 ggccgcgctg gaactggacc ccataccctg gctgccgaca ttgcagcggc tgctggaagg
      781 catcgccgcc gcgcgcggcg aaaagctgcc caaacccaca tcaggcaaga cgaggagagc
      841 gacatgagca acgacaccgc gagcgccggg cgcatcgaga tcgacgggcg caagttcaac
      901 ccgtactcca aggacttcat ccgcaatccg cacgacacct ggcagatgct gatccgcgat
      961 tacccgatcg cctggcacaa ggatctgcag atgtggctgg tcaactcgca cgagctgaac
     1021 gaccgcatgc tcaaggacaa ccgcttctcg ccgaatttcc gcgagtggaa gctggcgccg
     1081 ccgctgaaag cggacgccga caagaaccat ttcgacctgg cgctggagaa gggattcttt
     1141 gccgtcgacc agaaggcgca cctgcgcctg cgcaagctga ccatgccggc cttctccaag
     1201 ccggtgatgg gcaagatcga cgccaagatc cgcgacctgg tcacgcagtg cttcgatgcc
     1261 atcggcgacg ccgaggagtt cgacgtctac caggccatcg ccagcaagct gccggtgcgc
     1321 gccatcgccc gcatgatcgg cgtggcggtg gacaacgaag acttcttcca cgacttcgcg
     1381 gtcaacgtga tcaagggcac ccgcatcaac ctgccgatca aggagcgcaa cgcggccatc
     1441 gaaaactcct tgcccggctt ccagttcttc ctcgaccaga tcaaggcccg ccgcgcgcag
     1501 gcgacacccg gcgacgattt cctcggcagc ctggtcggct cggtggtcga cggcgagcgc
     1561 ctcgacgatt acgaaatcct ctcggtggtg ttcgcgctga tcaccgccgg ctcggataca
     1621 gcgactgacc tgcacacctt cgccatcgac ggcctgctgc gcaacccgga ccagtacgag
     1681 ctgctcaaga acaaacccga gatcatggag aacgccatca tcgagctgct gcgctacggc
     1741 tcgttcggca agatgccgtt cttccgcttt gccaccggcg actatgaatt tgccggccag
     1801 cacattgcca agggcgaagc catcggcgtc aacgtttccg ccgcgtggaa cgatccggcg
     1861 aagttccccg actggaacaa gctggacctg cagcggcgcc tggacggcaa cctggtgttt
     1921 ggcgcgggcg cgcatttctg catcggcacc tacctggtgc gcgtgcaggg cggactgatg
     1981 ctcaccgagt tcatgaagcg cttccccaat gccacgctgg cggatggcaa cgggcacgtc
     2041 gactacgaat acactcacca caacgcgcgg cgcatcaaca agctggtggt gcggaccaac
     2101 cgtgcaaaga tgcagaaggc ggcctgatgg acaaatacat ctgcgtgatc tgcggtttca
     2161 tctatgaccc ggcggtcggc ctgccgcagc acggcattgc cgccggcacg cgcttcgagg
     2221 acctgccggc cgactggatc tgcccggagt gcggctcgcc caaggaatcg ttcgagatct
     2281 acacgggctg agcgagggct cctgcaaacg cagtaggttg atgcattgca gcactaatcg
     2341 gtttggatga tgcactcact tcgtgcgccg ctaggatttt gccggcgcaa ggatggcaat
     2401 ggcgggggcc gctgctaaca tcgggcgcac aagtttttgc cgggcgttgc acggccaaaa
     2461 cgaaactaga actgtttgtt gcgctcatgt gaggagatca ccaatgaagc tcaagcttgt
     2521 gctgacctcg tgcgtcgccg ccgtcacgtt ctgtgcggcg caggccacgc tggccaaggt
     2581 gccgccggaa aaggcggcgg aactcgacgg ccccaagtac acctgcatgg gcgcggaacg
     2641 cgccggcaac ggcaaggaca tccccgaata cacgggcaag ttccagggca gctggcccgg
     2701 catgaagggc aagtcgggct tcgagcccgg cccgtatgcc gacgagaagc cgctgttcac
     2761 catcagcgcc gccaacatgg cgcagtacgc cgacaggctc actgagggtg agaaggcgct
     2821 gatgaagaag taccccgaca actacaagat gaacgtgtac ccgagccacc gcgatttcgc
     2881 cgagcccaag tgggcctgcg acgcgaccaa gaaaaacgtc ggcacctcgg agatcatcca
     2941 caacggcctg ggcatcaccg gcgtggctgg cggcgtcgcc ttcccgttcc cgcagagcgg
     3001 cctggaagcg atctggaacg cgatcaatcc gccgcgcgtg tacaacgagg aagccatctg
     3061 cgacgtggcc gacgtctacg ccaacggttc catcgcctgg ggccgcaacc acttcaaggt
     3121 gctggcgctg accttcgatc cgaaccaccg cggctcgttc caggacaagg tcaactccta
     3181 cttctacacc agctacctgc tgcccgagcg cgacaagggc ttcaccgcgg tgggctacca
     3241 gcccaacgat ttcaccgacg actcgacgca gagctggcag tacctgccgg gcctgcgccg
     3301 cgtgcgccag gcgccggaag tgggcttcga ctacccggtg ccgccggccg gcctgcgcac
     3361 ggtggacgac gactatggct tcaacggttc gccggagcgc tacacctgga agctggtcgg
     3421 caagaaggaa atgtacgtgc cgtacgacaa cttcaaggtc aatgatccgg cggtgaagta
     3481 caaggacctg atcaagcccg gcagcgtcaa tcccgagtac gagcgctacg aggcgcaccg
     3541 cgtgtgggtg ctcgagggca cgctcaaacc cggcgtgcgc cacatctaca gcaagcgcct
     3601 gctctacgtc gacgaggatt cctggctgac gctgatggcg gacaactacg atgcgcgcgg
     3661 ccagctgtgg cgcgtggcgc tggtcaactt ccattactcg caggagtcgg gcacctggca
     3721 tcgcggcgcg accatctacc acgaccttac ctcgggcgcg tatgaagccg gttacctgac
     3781 caacgaggcg ggcgagcact ggtggaagat caaccagccg ggcatgaccg acgcgcagtt
     3841 caagccggaa gcggcggcgc gcggcggaca ctgaagaagg acatcaccga ccagggcggc
     3901 atggccgccc tgatcttttt ttaaggaccg agcatgaaga ctgcattcgt caccggggcc
     3961 aacgtcggcc aggccaacct gctggtgaag gcgctggcgc agcgcggctg gcgcgtgttc
     4021 gccggcgtgc tgccgggcgc gcccaccgac ctgaagagcg aaggcaacat caccgtcgtc
     4081 gaccaggacg tgtcgaagac cgattcggtc aaggccagcg ccgaggtggt gaccaaggcc
     4141 ttgaacggcg gcggcctcga cctgctgatg aacgtggcgg gcgtcgccaa catcgccgtc
     4201 ggcgtagtgg aaggcgccga cctcgcgcaa gccgagcggc tgttccagat caacctgttc
     4261 ggccagctgc gcgtgatcca gtccttcctg ccgctggtgc gcaggaatgc gccgggctcg
     4321 cgcatcgcca actattcatc gggcgccatc ctggccaacc cgatcggcgc cggcatctac
     4381 aacatgacca agcacgccat ccacggcatg acgctgaccc tgcgccacga gctggcgccg
     4441 ctgggcatcc aggtcacctc gatcatcccc ggcggcgtgc tcaccggcat gagcgccaac
     4501 agccaccaga acacgcgtaa cagctggaac gcgcagcgcg aggatctgcg ccaggtgtac
     4561 cagccgctgg aaaacgtcat gtgccaggtg ctgcccgaca tgctggagaa gagcggcagc
     4621 acgcccgagc aggcgctggc cggcacgctg gaaatcctcg acaagaaaaa gtggaagccg
     4681 atggaatacc tgggcaagga cgtgaagccg atgggcatca tgcgccgcat cctgtcggac
     4741 tcgcagctgg aatcgatgat gcgcggggcg ttcaagatcc ccgcgtacaa ggcgtaagga
     4801 gcagcgcatg aacgcagtcc tcaaggaatc tcccagcgat atttccgcca tcttcacggg
     4861 tctggcggcc aaggcgccgg agctggcgca gaccagcgcc gcgcagcgcg ccgacaagct
     4921 gcgccggctg cttgcggcgg tgatggccgc gcgcccggcg atcctcgcgg ccggcaagaa
     4981 ggaactgcgc ctggtcgacc tcgacatcga tgcgcagctg atgatggtca agggcgagat
     5041 cgaattcgcc tgcaagcatc tcgcgcaatg gatggcgccg gaagacatcc agccgaccat
     5101 gatgacgctg ggcaagcgca gttacgtgaa atacgaaccc aagggcgtgg tgctcaacct
     5161 cgccacctgg aacgcgccga tcgccatcgc cttcgtgccg ctggtgggcg cgattgccgc
     5221 cggcaacgcg gtgctgctca agcccagcga actggcgccg cactcggcgc aggtgctggc
     5281 cgacattgtc gccacggtgt ttccgcccga cgaattcgcc gtggtgcagg gcggcccgga
     5341 agtggcgcag gaagtgctgc gccagccgtt caaccacatc tactacatcg gcggccatgc
     5401 ggtcggccgg ctggtgatga aggcggcggc ggagcatttc gcgtcggtga cgc
//
LOCUS       DASZZC010000529         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_949213,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000529 DASZZC010000000
VERSION     DASZZC010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949213"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VHE37_11255"
     CDS             <1..359
                     /locus_tag="VHE37_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HVT36158.1"
                     /translation="AAASLLLITNGRIAGISGLYSAAWQRRDVTALLFMLGLVAAPWL
                     TRAFGLAPPVLELQVLRIALAGLLVGIGTAIGNGCTSGHGVCGLANLSPRSLAATLTF
                     MAVAAMVVALHLGERL"
     gene            356..790
                     /locus_tag="VHE37_11260"
     CDS             356..790
                     /locus_tag="VHE37_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HVT36159.1"
                     /translation="MKFSALLAGLLFGVGLVLSGMTDPHRVIGFLDFFGAWDPSLAFV
                     MGGAVLTALPAFAYARRHERTPTGVAFPRIDRMRIDARLLGGAAIFGAGWGLAGLCPG
                     PGLVQAGAGSAGGWIFIAGIALGAWLASRYDAARAKNATTAP"
     gene            924..>1992
                     /locus_tag="VHE37_11265"
     CDS             924..>1992
                     /locus_tag="VHE37_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT36160.1"
                     /translation="MLHPGPLSIVVAGKLPRSQAPLLKHIEKNFPNVRVHAADNEKEA
                     AEFYQQHHSQLTLCDVSTFGITIEKVAALLREVNVDSRIVAVAEPESAPDIVQTIARG
                     ARDSISTRDPLHAMLVLAREMEILPDWCARNAQLLEGRIGKEAAAHAVGDDIVWCNDA
                     FLLAMARKAREDLMHHAFWNCIDGDDHYKLKRALQASAAGQPPKSPVEVRALAADGKR
                     IALSLRLSQKQADGGTVVHIAAREISMTGVPAAYGGQINVAVLKRLQAAVSGGGLALA
                     LQPITPLADAGGGDVSKLDVLARIKDHDGELAAAEFMREASAAGLLRQIDHWMIRTAC
                     AYYARNAAKGEVLLFLRLSRQA"
BASE COUNT          296 a          682 c          693 g          321 t
ORIGIN      
        1 gcgccgccgc ctcgctgctg ctgatcacca acggccgcat cgccggcatc agcggcctgt
       61 acagcgcggc gtggcagcgg cgcgacgtga ccgcgctgct gttcatgctc gggctggtcg
      121 ccgcgccgtg gctgacgcgc gcgttcggcc tggcgccgcc ggtgctggaa cttcaggtgc
      181 tgcgcatcgc gctcgcgggt ttgctggtcg gcattggcac cgccatcggc aacggctgca
      241 ccagcggcca cggcgtgtgc gggctggcga atctttcgcc gcgcagcctc gcggccacgc
      301 tcactttcat ggcggtggcg gcgatggtcg ttgcgctgca cctgggagag cggctgtgaa
      361 attttccgcg ctgctcgctg ggctgctgtt cggcgtcggg ctggtgctgt cgggcatgac
      421 cgatccgcac cgcgtaatcg gctttctgga tttcttcggc gcgtgggacc catcgctggc
      481 cttcgtcatg ggcggcgcgg tgctcaccgc gctgccggca tttgcctacg cgcgcaggca
      541 cgagcgcacg ccaaccggcg tcgcctttcc gcgcatcgac cgcatgcgca tcgacgcgcg
      601 gctgctgggc ggcgcggcga ttttcggcgc cggctgggga ctcgccggac tctgcccggg
      661 accgggcctg gtgcaggcgg gcgccggcag cgccggtggc tggatcttca tcgccggcat
      721 cgcgctgggt gcgtggctgg cctcgcgcta cgacgctgcg cgcgcgaaaa atgccaccac
      781 cgcaccctga ttcttgctgt tattaattat tgatcacatt ttattttaaa cgataattaa
      841 taacacacca aggacgggtg cggtggcagg attttttcgc gcttgacggc aacgcgcgtc
      901 accggcaaag cttcggcctc ctcatgctcc atcccggccc gctctccatc gtcgtcgctg
      961 gcaagctgcc gcgctcgcag gcgccgctgc tcaagcacat cgagaagaat ttccccaacg
     1021 tgcgcgtgca cgcggcagac aacgagaagg aagccgccga gttctaccag cagcaccact
     1081 cgcagctgac gctgtgcgac gtctccacct tcggcatcac catcgagaaa gtggcggcgc
     1141 tgctgcgcga ggtcaacgtg gactcgcgca tcgtcgcggt ggccgagccg gagagcgcac
     1201 cggacatcgt gcagaccatc gcgcgcggtg cgcgcgacag catcagcacc agggacccgc
     1261 tgcacgccat gctggtgctg gcgcgcgaga tggagatcct gcccgactgg tgcgcgcgca
     1321 acgcgcagct gctcgagggc cgcatcggca aggaagcggc ggcgcacgcg gtgggcgacg
     1381 acatcgtctg gtgcaacgac gctttcctgc tggcgatggc gcgcaaggcg cgcgaagacc
     1441 tgatgcacca cgccttctgg aactgtatcg acggcgacga tcactacaag ctcaagcgcg
     1501 cgctgcaggc cagcgccgcc ggccagccgc cgaaaagccc ggtggaagtg cgcgcgctgg
     1561 ccgcggacgg caagcgcatc gcgctgtcgc tgcgcctgtc gcagaaacag gccgacggcg
     1621 gcaccgtggt gcacatcgcc gcgcgcgaga tcagcatgac cggcgtgccc gcggcctacg
     1681 gcgggcagat caacgtggcg gtgctcaagc gcctgcaggc agcggtcagc ggcggcggcc
     1741 tggcgctggc gctgcagccg atcacgccgc tggccgacgc tggcggcggc gacgtgtcca
     1801 agctcgacgt gctggcgcgc atcaaggatc acgacggcga gctggccgcc gccgagttca
     1861 tgcgcgaggc cagcgcggcc gggctgctgc ggcagatcga ccactggatg atccgcacgg
     1921 cctgcgccta ctacgcgcgc aacgcggcca agggcgaggt gctgctgttc ctgcgcctgt
     1981 cgcgccaggc at
//
LOCUS       DASZZC010000530         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1259101,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000530 DASZZC010000000
VERSION     DASZZC010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259101"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VHE37_11270"
     CDS             complement(<1..663)
                     /locus_tag="VHE37_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36161.1"
                     /translation="MTQKIALVTGATRGIGYETVRQLARAGVHVLLAGRKRQQAVDAA
                     LTLQKEGLAVEAIALDVTDAASISAAAQEVQRKHGRLNILVNNAGILVDTFGKKPSEQ
                     TLDTWRKTFDTNLFGLIAVTQAFLPLLHQSAAGRIVNVSSILGSLALHSDPKSPIYDF
                     KVPAYNVSKSAVNAWTVQLAYELRGTPVRVNTIHPGYVKTDMNKGEGELEIADGARTS
                     VQL"
     gene            complement(695..>1793)
                     /locus_tag="VHE37_11275"
     CDS             complement(695..>1793)
                     /locus_tag="VHE37_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT36162.1"
                     /translation="GLGYGELQTAEQTLAGSIAKAPGLSFPISSYQANVPQLNAQVDR
                     AKAKAQGVALSDVFQTLQVYLGSIYVNDFSRFGRTFQVIAQADAPFRNRVENIGELRT
                     RNAAGQMVPIGSLVNLSQTYGPDPVIRYNGYPAADLIGQADPRLLSSNQAMAAVSEIA
                     QKVLPNGMTLEWTDLSYQQSTQGKAALVVFPLSVLLVFLVLAALYESWTLPLAVILIV
                     PMSMLAALTGVWLSGGDNNIFVQIGLVVLMGLSSKNAILIVEFARDLERQGRSAVAAA
                     IEATHLRLRPIVMTSMAFIAGVMPLVYSHGAGAEVRHATGITVFAGMLGVTLFGLFLT
                     PVFYVALRKLALRSAPRAEATPITGAALLQH"
BASE COUNT          302 a          577 c          604 g          310 t
ORIGIN      
        1 cagctgcacg ctggtgcgcg cgccatcggc aatttccagt tcgccttcac ccttgttcat
       61 gtcggtcttc acgtagccgg gatggatggt gttgacgcgg accggcgtac cgcgcagttc
      121 gtacgccagc tgcacggtcc aggcgttgac cgcgctcttc gacacgttgt acgccggcac
      181 tttgaagtcg tagatcggcg acttcggatc gctgtgcagc gccagcgagc ccagaatgct
      241 ggagacgttg acgatgcggc cggcggcgga ctggtgcagc agcggcagga aggcctgcgt
      301 caccgcgatc agcccgaaca ggttggtgtc gaaggtcttg cgccaggtgt cgagcgtctg
      361 ctcggacggc ttcttgccga aggtatcgac gagaatccct gcattgttga ccaggatgtt
      421 gagacgtccg tgcttgcgct gcacctcctg cgcagcggct gaaatgctcg ccgcgtcggt
      481 gacgtcgagc gcgatggctt cgacggcgag tccttctttc tgcaaggtca gcgcggcgtc
      541 caccgcctgc tggcgcttgc gtccggccag cagcacgtgc acgccggcgc gcgccagttg
      601 gcgcaccgtt tcatagccga tgccgcgggt ggcgccggtg acgagggcaa tcttctgggt
      661 catgtgaatt ctccgtttga agtggacagg aatctcagtg ctgcaggagg gcggcgccgg
      721 tgatcggcgt cgcttcagcg cggggagccg agcgcagcgc cagcttgcgc agcgccacgt
      781 aaaacaccgg cgtcaggaac aggccgaaca gcgtcacgcc gagcatgccg gcgaacaccg
      841 tgatgccggt tgcgtggcgc acttccgcgc cggcgccatg cgaatacacc agcggcatga
      901 cgccggcgat gaaagccatc gaggtcatca cgatgggccg caggcgcagg tgcgtggcct
      961 cgatcgccgc ggccacggcg ctgcggccct ggcgttccag atcgcgcgca aactcgacga
     1021 tcaggatggc gttcttcgac gacaggccca tcagcacaac caggccgatc tgcacgaaga
     1081 tgttgttgtc gccgccgctg agccacacgc cggtgagcgc cgcgagcatc gacatcggca
     1141 cgatcaggat cacggccagc ggcaatgtcc agctttcgta cagcgccgcc agcaccagga
     1201 acaccagcaa caccgacagc gggaacacca ccagagcggc cttgccctgg gtggactgct
     1261 ggtacgacag gtcagtccac tccagcgtca tgccgttggg caaaaccttc tgcgctattt
     1321 cggaaaccgc cgccatcgcc tggttggacg acagcagccg cgggtcggcc tggccgatca
     1381 ggtcggccgc cgggtagccg ttgtagcgga tcaccggatc cgggccgtag gtctgcgaca
     1441 ggttcaccag gctgccgatc ggcaccatct ggcccgcggc attgcgcgtg cgcagttcgc
     1501 cgatgttctc gacgcggttg cggaacggcg cgtcggcctg tgcgatcacc tggaaggtgc
     1561 ggccgaagcg gctgaagtca ttgacgtaga tcgagcccag ataaacctgc agggtctgga
     1621 acacgtcgct cagcgccacg ccctgcgcct tggccttggc gcggtcgacc tgggcgttga
     1681 gctgcggcac gttggcctgg tacgagctga tcgggaacga caggcccggc gccttggcga
     1741 tgctgccggc gagcgtctgc tccgcagtct gcaattcgcc gtagcccagg ccc
//
LOCUS       DASZZC010000531         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1009919,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000531 DASZZC010000000
VERSION     DASZZC010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009919"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VHE37_11280"
     CDS             complement(<1..1287)
                     /locus_tag="VHE37_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HVT36163.1"
                     /translation="MSGIVVVDQRSDWPVDVPGIETVTAWDYLTHETHTRARGNRVYN
                     LCRSFEYQSTGYYVSLLASARGQKPLPDITTIQDLKLAESPRVLNDEIEELIQSSLSR
                     IGSAEYVMNVYFGESLAKRHERLARALFNLFPAPLLQATFVYRDDEWRVDTLGPIALG
                     EVPTSHHEILYQAARDYFMRRRAPRVKRDSSRYDLAILVNDDEKEPPSNARALKAFEK
                     AAEDLGFEVEFLDKGDYGHVAEFDALFIRETTAVNHHTYRFSRRAAREGLVVVDDPVS
                     ILRAANKVFLAQLMERHRIPQPKTMLIHRGNVSHVARELGFPCVLKQPDSQFSKGVLK
                     AESETELKTMTREMLERSDLIIAQSFEPTEYDWRVGVLDGQPLYACRYYMAKDHWQVV
                     KRGKDGGKQEGNHETLDVREVPPGVLNVAVQAARAVG"
     gene            1625..>2355
                     /locus_tag="VHE37_11285"
     CDS             1625..>2355
                     /locus_tag="VHE37_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36164.1"
                     /translation="MMLAACLPATALAALPLQVQRVAAPAQLLHAGKKTLLTPRKPIE
                     AGDRLVVGHIGRVGLRLVPSGELLLGEDSELFVHSAEPGTAGGGTVVRLALTHGSLHL
                     DSLPRDGQPAEDMRLNVGTLRVRVLGAEVWAGAEPGVAETVCLLQGTVDIATDFGNEH
                     LDTPGDCLRFGANHVRMLLRPENDGVLARKLARTTFAGDPPLAAPPAAVAMPRPPPSA
                     PPTPLVQQAAEPPPAVTPAEPAPAAV"
BASE COUNT          341 a          793 c          799 g          422 t
ORIGIN      
        1 gccgactgcg cgcgccgcct gcaccgccac gttgagcacg cccggcggca cctcgcgcac
       61 gtccagcgtc tcgtggttgc cctcctgctt gccgccgtcc ttgccgcgct tgaccacctg
      121 ccagtggtcc ttggccatgt agtagcggca ggcgtacagc ggctggccgt cgagcacgcc
      181 gacgcgccag tcgtactcgg tgggttcgaa cgactgcgcg atgatcaggt ccgagcgctc
      241 cagcatctcg cgcgtcattg tcttgagctc ggtctcgctc tccgccttca gcacgccctt
      301 ggaaaactgc gaatccggct gcttgagcac gcaggggaag ccgagttcgc gcgccacgtg
      361 gctgacgttg ccgcggtgga tcagcatggt cttcggctgc gggatgcggt ggcgctccat
      421 cagctgcgcc agaaaaacct tgttcgccgc acgcaggatc gacaccgggt cgtccaccac
      481 caccagccct tcgcgcgcgg cgcggcggct gaagcggtag gtgtggtgat tgaccgcggt
      541 ggtctcgcgg atgaacagcg cgtcgaactc ggccacgtgg ccgtagtcgc ccttgtcgag
      601 aaactccact tcgaagccga ggtcttccgc cgccttctcg aacgccttca gcgcgcgcgc
      661 attggacggc ggctcctttt catcgtcgtt gaccaggatg gccaggtcgt agcgcgagct
      721 gtcgcgtttc acccgcggcg cacgccggcg catgaagtag tcgcgcgcgg cctggtagag
      781 gatttcgtga tgcgaggtcg gcacctcgcc cagcgcgatc gggcccagcg tgtccacgcg
      841 ccactcgtcg tcgcggtaga cgaaggtggc ctgcagcagc ggcgcgggga acaggttgaa
      901 cagcgcgcgc gccagccgct cgtggcgctt ggccagcgac tcgccgaaat acacgttcat
      961 cacgtactcg gccgagccga tgcgcgacag cgagctctgg atcagctcct cgatctcgtc
     1021 gttgagcacg cgcggcgatt cggcgagctt caggtcctgg atggtggtga tgtcgggcag
     1081 cggtttctgt ccgcgcgcgc tggccagcag cgacacgtag tagccggtcg actggtattc
     1141 gaacgagcgg cacaggttgt acacgcggtt gccgcgcgcg cgcgtatgtg tctcgtgcgt
     1201 gaggtaatcc caggcggtga cggtctcgat gcccggcacg tccaccggcc agtcgctgcg
     1261 ctgatcgaca acgacgatcc cgctcatgca gacctatggc ggtcggcgcc ggcaggcaag
     1321 gggtcttcgg atgcttccgg catgtgccgt gtttttgcgg tgcgcgtttg cagtggtggt
     1381 atcgcagcgc ggcgcatcct agcacgatca agtttcatct ccggccggtt ttccgctcca
     1441 ccgcactgcc gtcgaccatg ttatttaaaa tataattagt tttagtttat ttaataatta
     1501 aaaacatcaa gaataggggt gcggtgcaga gctttgtgcg acttttccca tgcgcagcgt
     1561 gccgcccttg cggcgcgtgg gcctgctgca cgatcatcgg gccatgcgtg ctcttatccg
     1621 gggaatgatg ctcgctgcct gcctgccggc caccgcgctg gcggcgctgc ccttgcaggt
     1681 gcagcgcgtg gcggcgccgg cgcagctgct gcatgcgggc aagaaaacct tgctcacgcc
     1741 gcgcaagccg atcgaggctg gcgaccggct ggtcgtcggc cacatcggcc gcgtcggcct
     1801 gcgcctggtg cccagcggcg aactgctgct gggcgaggat tccgaactgt tcgtgcacag
     1861 cgccgagccg ggcaccgccg gcggcggcac cgtggtgcgc ctggcgctga cccacggcag
     1921 cctgcacctg gacagcctgc cgcgcgacgg ccagccggcc gaagacatgc gcctgaacgt
     1981 cggcacgctg cgcgtgcgcg tgctcggcgc ggaagtgtgg gccggtgccg agccgggcgt
     2041 ggccgagacg gtctgcctgc tgcagggcac cgtggatatc gccactgatt tcggcaacga
     2101 gcacctggac acgccgggcg attgcctgcg cttcggtgcc aaccacgtgc gcatgctgct
     2161 gcgcccggag aacgatggcg tgctggcgcg caagctcgcg cgtaccacct tcgccggcga
     2221 tccgccgctg gccgcgccgc cggccgcggt ggcgatgccg cgtccgccgc cctccgcgcc
     2281 gcctacgccg ctggtgcaac aggccgcgga gccaccgccg gcagtgacac cggccgaacc
     2341 ggcgcctgct gcggt
//
LOCUS       DASZZC010000532         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_298323,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000532 DASZZC010000000
VERSION     DASZZC010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298323"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VHE37_11290"
     CDS             complement(<1..656)
                     /locus_tag="VHE37_11290"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HVT36165.1"
                     /translation="MRKYISVPQVEGQASRQAHADLPAGAVERELGKEGFFGPASHMI
                     HPKPPTGWTSWEGPLRPRAFDCVKLGESGPSPWNAQELLFNANVRYRWWRCAQTMDHL
                     ARNADGDELLFIHTGHASLYCDYGHLELTGGDYLVLPRGTMWRIECSAPVELLLIEAT
                     NSTYSLPDKGLVGEHAIFDPAMLDRPRMDESFRAQQQGEIAAREWRVSIKRNNLLSTV
                     T"
     gene            complement(649..>1595)
                     /gene="hppD"
                     /locus_tag="VHE37_11295"
     CDS             complement(649..>1595)
                     /gene="hppD"
                     /locus_tag="VHE37_11295"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HVT36166.1"
                     /translation="VARHRSKDVTLFRQGDINFLVNGTPYTHFAQFAQAHGPSACAMG
                     WRVRDAVRAHAHALQQGAVDFDTRPGYMELKLPAVYGIGNSVLYFIDRYGPHSIYDVD
                     FRFFDGVDPAPRGVGLTEIDHLTHNVARGNMDTWAGFYERIANFRQIRYFDIEGKQTG
                     LFSRAMTSPCGKIRIPINESADDKSQIEEFLKQYRGEGIQHIALSTRDIYATVEALRA
                     RGVVFMDTPDTYYAKIDARLPKHGESVARLQSLRVLIDGAPEEGILLQIFTSTVIGPI
                     FFEIIQRKGNEGFGEGNFKALFESIEEDQIRRGVLADA"
BASE COUNT          245 a          508 c          538 g          304 t
ORIGIN      
        1 gtcaccgtcg acagcaggtt gttgcgcttg atcgacacgc gccattcgcg cgctgcaatt
       61 tcaccttgct gctgcgcgcg gaagctctcg tccatgcgcg ggcggtcgag catggcgggg
      121 tcgaaaatcg cgtgctcgcc gaccagtccc ttgtccggca gtgaatacgt gctgttggtc
      181 gcctcgatca gcagcagttc caccggcgcg ctgcattcga tgcgccacat ggttccgcgc
      241 ggcagcacca ggtaatcgcc gccggtgagt tcgagatggc catagtcgca gtacaggctg
      301 gcgtgtccgg tatggatgaa cagcagctcg tcgccgtcgg cgttgcgcgc caggtgatcc
      361 atggtctgcg cgcagcgcca ccagcggtag cgcacattgg cgttgaacag gagttcctgc
      421 gcgttccacg gcgacggccc ggactcgccg agcttcacgc agtcgaacgc gcgtgggcgc
      481 agcgggcctt cccacgaggt ccagccggtg ggcggtttgg gatggatcat gtgcgaggcc
      541 gggccgaaga agccttcctt gcccagctcg cgttccaccg cgccggccgg caggtcggca
      601 tgcgcctggc ggctggcctg gccttcgacc tgcggcacgc tgatgtattt acgcatcggc
      661 cagcactcct cgacgaatct gatcctcctc gatcgattcg aagagtgcct tgaaattgcc
      721 ttcgccgaaa ccctcgttgc ccttgcgctg gatgatctcg aagaagatcg ggccgatcac
      781 cgtgctggtg aagatctgca gcaggatgcc ttcctccggc gcgccgtcga tcagcacgcg
      841 caggctctgc agccgcgcca cgctctcgcc gtgtttgggc agccgcgcat cgatcttcgc
      901 gtagtaggtg tcgggcgtgt ccatgaacac cacgccgcgc gcgcgcaacg cctcgaccgt
      961 ggcgtagatg tcgcgcgtgg acagcgcgat gtgctggatg ccctcgccgc ggtactgctt
     1021 gaggaattcc tcgatctggc tcttgtcgtc cgcgctctcg ttgatcggga tgcggatctt
     1081 gccgcacggc gaggtcatgg cgcgcgagaa caggccggtc tgcttgccct cgatgtcgaa
     1141 gtagcggatc tgcctgaagt tggcgatgcg ctcgtagaag ccggcccagg tgtccatgtt
     1201 gccgcgtgcg acgttgtgcg tcaggtgatc gatctcggtg aggccgacgc cgcgcggtgc
     1261 gggatcgacg ccgtcgaaga acctgaagtc gacgtcgtag atcgagtgcg ggccgtagcg
     1321 gtcaatgaag tacaggacgc tgttgccgat gccgtagacc gccggcagct tcagttccat
     1381 gtagccgggc cgcgtgtcga aatccaccgc gccctgctgc agcgcatgcg catgcgcgcg
     1441 caccgcgtcg cgcacacgcc agcccatggc gcaggcgctg gggccatgcg cctgtgcgaa
     1501 ctgggcgaag tgcgtgtacg gcgtgccgtt gaccaggaag ttgatgtcgc cctgacggaa
     1561 cagggtcacg tccttgctgc ggtggcgcgc caccg
//
LOCUS       DASZZC010000533         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_620853,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000533 DASZZC010000000
VERSION     DASZZC010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620853"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..963
                     /locus_tag="VHE37_11300"
     CDS             322..963
                     /locus_tag="VHE37_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT36167.1"
                     /translation="MDFLTHILSFFQKGGPFMYPIALVLALGLGIAIERFVLLRKAAR
                     ENGALWKELQPLLDQRDFESAERVASGSDTAVGRVMTSALAHARSNGASRGELEVAVE
                     ESLMEVTPLIEKRTHYLATLSNMSTLLGLLGTVIGLIGAFAALAHADAAEKADLLSAG
                     IAEAMNCTAFGLMVAIPFLLLHSYLQTRTNELVDGIEMCCMKLINARTASAKR"
     gene            995..1528
                     /locus_tag="VHE37_11305"
     CDS             995..1528
                     /locus_tag="VHE37_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT36168.1"
                     /translation="MKSNHRIRRMQRHNRARAAAVHLNIVSMIDVFAVLVFFLLLSAS
                     LAADRLATLGLDLPSENAPKVETPPPKVLQVTVHRDGRLDLQDRAGNVTQIPGTTEGH
                     DLETLANDLLAIKQAAPKEESITLLLEPDVPYDVLVQVMDTARNAPGEAIFENGSHDM
                     FPLISIGDAPSAQSGAP"
     gene            1525..2034
                     /locus_tag="VHE37_11310"
     CDS             1525..2034
                     /locus_tag="VHE37_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT36169.1"
                     /translation="MRSPRKVRRIERQYVQGGRAVPINIVSMIDIFAILVFYLLVSIA
                     AVEMRPNDASLQLPKSSSREHPPQATQLMITTKEILVDNKAVMPTLDAQNFSGDVLPT
                     LKDALESKVELKIIEGDQAGGLTRGDVNILADKTIPYSLVKKVMATCSQAKFARSSLA
                     VVERKGARK"
     gene            2031..>2965
                     /locus_tag="VHE37_11315"
     CDS             2031..>2965
                     /locus_tag="VHE37_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HVT36170.1"
                     /translation="MSIFELGRWEYAETAEARYRRVMTWVMVAYLVLGVLIPFLKLIG
                     VQEGGGESGKQRYAKLVQAQPAPVAPKAEEPPPAPEPTPEPPKPQPQKPREVKPEPTF
                     EQRQQQARVVAQHSGLMAFADQLADLRDRNITSVDGSRPLTSAAPATSGSTAPDSSQM
                     FAQSASQTSGGIVTSATGAGRRQSGTQLDQRRTTTVQSPVGFGKDQTKPGQGGDKLIA
                     GRTLEEIQLAFDRAKSAFYIIFDRAARNNPNITTGKIVVSLTIAPDGQVTDCHVVSST
                     FGDPDLENKIVQRVKLINFGAKDVPVFTYPNYPIN"
BASE COUNT          583 a          988 c          941 g          453 t
ORIGIN      
        1 agaaggacat cgaccggccg gcacggctgc tgcaagagga attgctaccg atggacgaag
       61 acgtgttcat ccgccaggtc gagtactacg aagcgctggc gggtgcgctc aagcagaaga
      121 acgcggtgac gccgccggtc aagctcgtcc cggtggctcc gtgagcttgc accgcacacg
      181 ccaatttgat gttataattt gtagtatgtt tttattttca tataattttt catcacaagc
      241 aaaaaccggg tgcggtggat caaaaaatcc gctgatcgcg gcctccacac tccaatcgag
      301 caacacctga ggattcacgc aatggacttc ctgacccaca tcctgagctt cttccagaaa
      361 ggcggccctt tcatgtatcc gatcgcgctg gtgctggcgc tcggcctggg tattgccatc
      421 gagcgcttcg tgctgctgcg caaggcggcg cgcgagaacg gcgcgctgtg gaaggaactg
      481 cagccgctgc tcgaccagcg cgacttcgaa tcggccgagc gcgtggccag cggttccgat
      541 accgcggtgg gtcgcgtgat gacctccgcc ctcgcccacg cgcgcagcaa cggcgccagc
      601 cgcggcgaac tggaagtggc ggtggaagaa agcctgatgg aagtgacgcc gctgatcgag
      661 aagcgcactc actacctcgc cacgctgtcc aacatgagca cgctgctggg cctgctcggc
      721 accgtcatcg gcctgatcgg cgcgttcgcc gcgctggccc atgccgacgc cgcggagaaa
      781 gccgacctgc tgtccgccgg cattgccgaa gcgatgaact gcaccgcctt cggcctgatg
      841 gtcgccattc ccttcctgct gctgcactcg tacctgcaga cgcgcaccaa cgagctggtt
      901 gacggcatcg agatgtgctg catgaagctg atcaacgcgc gcacggcgtc ggccaagcgc
      961 taaccgaacc ggtcgaaatc acgagcaccc gcaagtgaag tcgaatcacc gcatccgccg
     1021 catgcagcgc cacaaccgtg cgcgtgcggc cgccgtgcac ctgaacatcg tgtcgatgat
     1081 cgacgtgttc gcggtgctgg tgttcttcct gctgctgtcg gcctcgctgg ccgccgatcg
     1141 cctggccacg ctgggcctgg acctgccgag cgagaacgcg ccgaaagtgg agacgccgcc
     1201 gcccaaggtg ctgcaggtga ccgtgcaccg cgacggccgg ctcgacctgc aggaccgcgc
     1261 cggcaacgtc acgcagatcc ccggcaccac cgagggccat gacctggaga cgctggccaa
     1321 cgacctgctg gcgatcaagc aggccgcgcc caaggaagaa agcatcaccc tgctgctcga
     1381 gcccgacgtg ccctacgacg tgctggtgca ggtgatggac accgcgcgca acgcgcccgg
     1441 cgaagccatc ttcgagaacg gatcgcatga catgttcccg ctgatctcca tcggcgacgc
     1501 gccgagcgcg cagtcaggtg cgccatgaga tcgccgcgca aggtgcgccg catcgagcgg
     1561 caatacgtgc agggcgggcg cgcagtgccg atcaacatcg tgtcgatgat cgacatcttc
     1621 gccatcctgg tgttttacct gctggtgtcg atcgccgcgg tggaaatgcg gccgaatgac
     1681 gcttccctgc agctgcccaa atcatcctcg cgcgagcatc cgccgcaggc cacgcagctg
     1741 atgatcacca ccaaggaaat cctggtcgac aacaaggcgg tgatgcccac gctcgacgcg
     1801 cagaatttct ccggcgacgt gctgcccacg ctgaaggacg cgctggagag caaggtcgag
     1861 ctgaagatca tcgagggcga ccaggccggc gggctgaccc gcggcgacgt caacatcctg
     1921 gccgacaaga ccattcccta ctcgctggtg aagaaggtga tggccacctg ctcgcaggcg
     1981 aaattcgcgc gcagctcgct ggcggtggtc gaacgcaagg gtgcgcgcaa atgagcatct
     2041 ttgaactggg ccgctgggaa tacgccgaga ccgccgaagc gcgctaccgg cgcgtgatga
     2101 cctgggtgat ggtggcctac ctggtgctgg gcgtgctgat cccgttcctc aagctgatcg
     2161 gcgtgcagga aggcggcggc gaaagcggca agcagcgcta cgccaagctg gtgcaggcgc
     2221 agcccgcgcc ggtggcgccc aaggcggaag aaccgccgcc tgcgccggaa cccacgccgg
     2281 agccgcccaa accgcagccg cagaaaccca gggaagtgaa gcccgagccc accttcgagc
     2341 agcgccagca gcaggcgcgc gtggtggcgc agcattcggg gctgatggcc ttcgccgacc
     2401 agctggccga cctgcgcgac cgcaacatca ccagcgtgga tggttcacgt ccgctcacct
     2461 cggcggcgcc ggccaccagc ggaagcaccg cgccggattc cagccagatg ttcgcgcaga
     2521 gcgcatcgca gacctccggc ggcatcgtca ccagcgccac cggcgccggg cgccggcaga
     2581 gcggcacgca gctggaccag cgcaggacca ccaccgtgca gagcccggtg ggcttcggca
     2641 aggaccagac caagccaggc cagggcggcg acaagctgat cgccgggcgc acgctggagg
     2701 aaatccagct ggcgttcgat cgcgccaagt cggcgttcta catcatcttc gaccgcgcgg
     2761 cgcgcaacaa tccgaacatc accaccggca agatcgtggt cagcctgacc atcgcgcccg
     2821 atggccaggt gacggattgc cacgtggtgt cgagcacctt cggcgacccg gacctggaaa
     2881 acaagatcgt gcagcgggtg aagctgatca acttcggcgc caaggatgtg ccggtgttca
     2941 cctaccccaa ctatccgatc aacta
//
LOCUS       DASZZC010000534         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_298439,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000534 DASZZC010000000
VERSION     DASZZC010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298439"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VHE37_11320"
     CDS             <1..138
                     /locus_tag="VHE37_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HVT36171.1"
                     /translation="KSIMGVMMLAAAKGSSITLIAEGADAQAALDELDALIARKFDEE
                     S"
     gene            146..1873
                     /gene="ptsP"
                     /locus_tag="VHE37_11325"
     CDS             146..1873
                     /gene="ptsP"
                     /locus_tag="VHE37_11325"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HVT36172.1"
                     /translation="MSLWLSGIGVSRGIAIARVQKMHGGDLDVPEYTLAPEDVEHETT
                     RFYDAHRRAREQLKEVREKIPLGTPGDIAAFIDTHILMMDDRSITEATVAHIRNSRCN
                     AEAALRRSRDALISVFEQMEDPYLKTRRDDVEHVVSRILRVLMKSERALPVKNEAGEM
                     FVIVADDVTPADIILLAQQGVAAFVTEFGGPLSHTAILARSLGIPAVVGLRDARKLLR
                     EGEQVIVDGEAGHVLADPDGQALDFYRAKRENLAQYRRDLEKIKADAAISRDGIKIHL
                     LANIELPEDAAEAAANGAEGVGLYRTEFLYMNRKGLPSEDEQYEAYARIVRSVKGPIT
                     IRTLDLGADKQVDSGRTHGPTPNNPALGLRAIRLCLKEPELFRAQARALLRASAHGRV
                     QIMLPMISSVQEFRQAHAVIDAAREELRREQVAMADHVPVGAMIEIPAAALAAPMLAR
                     QARFFSIGTNDLIQYTLAIDRVDDEVNYLYDPLHPAVLRLIQMTIDAGVQGGIPVAMC
                     GEMAGDRRYTRLLLGLGLMDFSMHPASVLEIKRIIIDSDIGELRRKVARLLTSADPNE
                     MRDLIAELD"
     gene            complement(1888..2697)
                     /locus_tag="VHE37_11330"
     CDS             complement(1888..2697)
                     /locus_tag="VHE37_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HVT36173.1"
                     /translation="MKTVPIYQIDVFAEKLFGGNPAGVCPLDAWLPDATMQAIAAENN
                     VAETAFFVKDESGAADFLLRWFTPTVEIELCGHATLASGYVLLEQRGFPRNVVRFRTL
                     KAGVLSVERSNGALWLELPARMPVPMKLVPGEVPRGLRAEPRAVLESGNKYLALFDHA
                     EDIVALKPDFAKLAQMNGKGVVVTAPGRKPGEDFVSRFFAPAMGVDEDPATGSAHCLL
                     VPYWAQRLGKTELEAQQLSARGAHFSCRLAGERVAMSGGVFPYLKGEIAVP"
     gene            complement(2715..3659)
                     /locus_tag="VHE37_11335"
     CDS             complement(2715..3659)
                     /locus_tag="VHE37_11335"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HVT36174.1"
                     /translation="MRSLARECEAAALAVDARITGSEGASVNTHRGSSLYGNSHGFRG
                     PRMGTHHSVSCAVVATDGDDMQRDYWYSSARRAADLEAAAAIGRRAGQRTVARLNGRP
                     LTTRKAPVLFVPELARGLWGSFLGAISGGALYRKSTFLLDKLDQPVFSPMVQLTQKPF
                     IPRGSGSAAFDGEGVAVRERTLVENGVLRGWLLGSYSARKLGLQTTGNSGGVFNLLVR
                     PGEKNFEQLVRDMGEGLVVTELMGQGVNPVTGDYSRGAAGFWVENGAIAYPVHEITIA
                     GNLLQMYGGIAALGSDVDETANTRTGSVLIDHMTIAGA"
BASE COUNT          629 a         1352 c         1238 g          535 t
ORIGIN      
        1 aagagcatca tgggcgtgat gatgctggcc gcggccaagg gcagcagcat cacgctgatc
       61 gccgaaggcg ccgatgcgca ggccgcgctg gacgaactcg acgcgctgat cgcacgcaag
      121 ttcgacgagg aaagctgagc gcgccatgag cctgtggctt tcaggaatag gcgtcagccg
      181 cggcatcgcc atcgcccgcg tgcagaagat gcacggcggc gatctcgacg tgcccgagta
      241 cacgctggcg ccggaagacg tcgagcacga aaccacgcgc ttctacgacg cgcaccggcg
      301 cgcgcgcgag cagctcaagg aagtgcgcga gaagatcccg ctgggcacgc cgggtgacat
      361 cgccgccttc atcgacacgc acatcctgat gatggacgac cgctccatca ccgaggccac
      421 cgtcgcccac atccgcaaca gccgctgcaa cgccgaggcc gcgctgcgcc gctcgcgcga
      481 cgcgctgatc tcggtgttcg agcagatgga agacccgtac ctgaagacgc gccgcgacga
      541 cgtcgagcac gtggtgtcgc gcatcctgcg cgtgctgatg aagagcgagc gcgcgctgcc
      601 ggtgaagaac gaggccggcg agatgttcgt catcgtcgcc gacgacgtca ccccggccga
      661 catcatcctg ctggcgcagc agggcgtggc cgccttcgtc accgagttcg gcggcccgct
      721 gtcgcacacc gccatcctgg cgcgctcgct gggcatcccc gcggtggtcg gcctgcgcga
      781 cgcgcgcaag ctgctgcgcg agggcgagca ggtgatcgtc gacggcgagg ccggccacgt
      841 gctggccgac cccgacggcc aggcgctgga tttctaccgc gccaagcgcg aaaacctggc
      901 gcagtaccgg cgcgacctgg agaagatcaa ggccgacgcc gccatcagcc gcgacggcat
      961 caagatccat ttgctggcca acatcgagct gccggaagac gccgccgagg ccgccgccaa
     1021 cggcgccgag ggcgtgggcc tgtaccgcac cgaattcctc tacatgaacc gcaagggcct
     1081 gccgagcgag gacgagcagt acgaggccta cgcgcgcatc gtgcgcagcg tgaagggccc
     1141 gatcaccatc cgcacgctgg acctcggcgc cgacaagcag gtcgacagcg gccgcacgca
     1201 tggcccgacg ccgaacaatc cggcgctcgg cctgcgcgcc atccgcctgt gcctgaagga
     1261 gcccgaactg ttccgcgcgc aggcgcgcgc gctgctgcgc gcctccgcgc acggccgcgt
     1321 gcagatcatg ctgccgatga tttcctcggt gcaggaattc cgccaggccc acgcggtgat
     1381 cgacgcggcg cgcgaggaac tgcgccgcga gcaggtggcg atggccgacc acgtgccggt
     1441 gggcgccatg atcgagattc ccgcggccgc gctggcggcg ccgatgctgg cgcggcaggc
     1501 gcgcttcttc tccatcggca ccaacgacct gatccagtac acgctggcca tcgatcgcgt
     1561 cgacgacgag gtcaactacc tgtacgaccc gctgcacccg gcggtgctgc ggctgatcca
     1621 gatgaccatc gacgccggcg tgcagggcgg catcccggtg gccatgtgcg gcgagatggc
     1681 cggcgaccgc cgctacacgc gcctgctgct cggcctcggg ctgatggatt tctccatgca
     1741 cccggccagc gtgctcgaga tcaagcgcat catcatcgat tccgacatcg gcgagctcag
     1801 gcgcaaggtc gcacgcctgc tgacctcggc cgatccgaac gagatgcgcg acctgatcgc
     1861 ggagctggac tgaatctccg ctgtgttcta cggcaccgcg atctcaccct tgagataggg
     1921 aaacaccccg cccgacatgg ccacgcgctc gccggcaagg cggcaggaga aatgcgcgcc
     1981 gcgcgccgac agctgctgcg cttccagctc ggtcttgccc aggcgctgcg cccagtacgg
     2041 caccagcagg caatgcgccg agccggtggc cgggtcttca tccacgccca tggccggcgc
     2101 gaagaagcgc gagacgaaat cctcgccggg cttgcggccg ggcgcggtca ccaccacgcc
     2161 cttgccgttc atctgcgcca gcttggcgaa atccggcttc agcgcaacga tgtcttccgc
     2221 gtggtcgaac agcgccaggt acttgttgcc cgactcgagc actgcgcgcg gctccgcgcg
     2281 caggccgcgc ggcacttcgc ccggcaccag cttcatcggc accggcatgc gcgccggcag
     2341 ttccagccac agcgcgccgt tgctgcgctc caccgacagc acgcccgcct tcagcgtacg
     2401 gaagcgcacc acgttgcgcg gaaagccgcg ctgctccagc agcacgtaac ccgacgccag
     2461 cgtggcgtgg ccgcacagct cgatctccac cgtgggcgtg aaccagcgca gcaggaaatc
     2521 ggctgcgccg ctttcgtcct tcacgaagaa ggcggtctcg gccacgttgt tctcggcggc
     2581 gatggcctgc atggtggcgt cgggcagcca ggcgtccagc gggcacacgc cggccgggtt
     2641 gccgccgaac agcttttccg caaacacgtc gatctggtaa atcggcacgg ttttcatctg
     2701 cgtccccccg cgcgtcatgc gccggcgatg gtcatgtggt cgatcagcac cgagccggtg
     2761 cgcgtgttag ccgtctcatc cacgtcgctg cccagcgcgg cgatgccgcc gtacatctgc
     2821 agcaggttgc cggcgatggt gatctcgtgc accgggtagg cgatggcgcc gttctccacc
     2881 cagaacccgg ccgcaccgcg cgaatagtcg ccggtcaccg ggttgacccc ctgccccatc
     2941 agctcggtga ccaccaggcc ctcgcccatg tcgcgcacca gctgttcgaa atttttctcg
     3001 cccggccgca ccagcaggtt gaatacgccg ccggaattgc cggtggtttg caggccgagc
     3061 ttgcgcgccg agtagctgcc gagcagccag ccgcgcagca cgccgttctc caccagcgtg
     3121 cgctcgcgca ccgccacgcc ttcgccgtcg aatgccgcgc tgccggagcc gcgcgggatg
     3181 aacggcttct gcgtgagctg caccatcggc gagaacaccg gctggtccag tttgtcgagc
     3241 aggaaagtcg acttgcggta cagcgcaccg ccggaaatgg caccgagaaa actgccccac
     3301 agcccgcgcg ccagttccgg cacgaacagc accggcgcct tgcgcgtggt cagcgggcgc
     3361 ccgttgaggc gtgccaccgt gcgctggccg gcgcgccggc cgatcgccgc cgctgcctcc
     3421 agatccgcag cgcggcgcgc gctggaatac cagtagtcgc gctgcatgtc gtcgccgtcg
     3481 gtggccacca ccgcgcagct cacgctgtga tgcgtgccca tgcgcggccc gcggaagccg
     3541 tggctgttgc cgtagagcga ggagccgcgg tgggtgttga cgctggcgcc ctcgctgccg
     3601 gtgatgcgcg cgtccaccgc cagcgccgcc gcctcgcact cgcgcgccag agaccgcacg
     3661 tccggccgac gagcccagca tagaaggaga gccgatgttc caggtcgttc aacagtgcgc
     3721 gaattgggca cacatacaga acagaaagcc cgcg
//
LOCUS       DASZZC010000535        11489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1197686,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000535 DASZZC010000000
VERSION     DASZZC010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197686"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHE37_11340"
     CDS             <1..264
                     /locus_tag="VHE37_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase"
                     /protein_id="HVT36175.1"
                     /translation="LFKSLARAQMSDQTTGMLKILFHSETLEVLGVHCFGDRASEIVH
                     IGQTVMASPAINNLRYFVNTTFNYPTMAEAYRVAAHNGLNRLA"
     gene            276..1574
                     /locus_tag="VHE37_11345"
     CDS             276..1574
                     /locus_tag="VHE37_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVT36176.1"
                     /translation="MRAAIPFLLLISISAAAEDLREAIDRLTPQVEPHVVANRRYLHE
                     HPELSNREVNTSKYVAQKLKALGIEVTTGVAKTGVVGVLRGGKPGPVVALRADMDALP
                     VVEEVDLPFKSTVRTSFDGKDVGVMHACGHDAHVGILLGVAEILSKLKGDLPGTVKFI
                     FQPAEEGAPLGEEGGAALMVKEGVMSNAPKPEVIFGLHVLSIYDVGQLAYRAGGAMAS
                     ADDFVIVVHGKQSHGAYPWLGTDPVVVASQIVLGLQTIPSRQMDLTKAPVIVTVGKIE
                     GGVRNNIIPDAVTLKGTLRSLDSEMRKQLQEKVKRTAEDIAASAGATAEVHIGEETSY
                     PVTYNDPGLTAAMLPTLQRVGGAGLTNAAVQMGAEDFSYFQQQVPGLYVFIGIRTPGA
                     PKDEFAPNHSPRFKIDESGLPLGVRTLANLTVDYMQQHAP"
     gene            1571..2590
                     /gene="ltaE"
                     /locus_tag="VHE37_11350"
     CDS             1571..2590
                     /gene="ltaE"
                     /locus_tag="VHE37_11350"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF007825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HVT36177.1"
                     /translation="MKLIDLRSDTVTRPTPGMLQAMGAAAVGDDVWGDDPTVNALQAK
                     VAERAGKEAGLFFPSGTQCNLAALMSHCERGDEYIVGQAAHTYKYEGGGAAVLGSIQP
                     QPIENAADGTLPLDKVIAAIKPPDDHFARTRLLALENTIGGQVLPAAYVSEVTALARQ
                     RKLATHLDGARIFNAAVASDSSLAELCAPFDTVSICFSKGLGAPVGSVLVGPRAQLAR
                     ARRWRKMLGGGMRQSGFLAAACLYALDHHVQRLAEDHANAARLARGLAQIAQVKVTAQ
                     HTNMVFVQFPEAHCARLEAQLRAQGMIMQALYASRLVTHLDVSADDVDRFVAAVQAYF
                     ASAQG"
     gene            complement(2625..3491)
                     /locus_tag="VHE37_11355"
     CDS             complement(2625..3491)
                     /locus_tag="VHE37_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT36178.1"
                     /translation="MRVLLYLLGHLPLSFLHALGAFAGNLLWVTHSSRRRVALRNLER
                     CMPGLTPAERRRMARASLGHEFKTYVETFRIWLGPAQPVKDAVREWRGIDVLERAFAQ
                     GKGVILLTLHQGAFEAVAIPMSANFPFYGLYKPQRGVIHALSLQGRCRFGGRMLKTEA
                     GVRKDSLVLLREKWGVYFMPDHDPPPGRGVFVPFMGQLAHTPTLVAKLAHESGAPVVF
                     MFGERLPHARGYIAHYIEAPAEISSGDVEAATAAMNRGLEACLRTCPDQYWWGYRRFR
                     RQPDGMPDFYAA"
     gene            complement(3687..4202)
                     /locus_tag="VHE37_11360"
     CDS             complement(3687..4202)
                     /locus_tag="VHE37_11360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HVT36179.1"
                     /translation="MRKILCAAGLATALCAAAAGDAPPAPAFTHMQAQDWLNSRPLTW
                     PELSGHVVMVEFWAFDCWNCYRSIPWINALRARHPALQVIGVHTPELEQEYVRDNVVR
                     KITELGVRHPVMIDNDYSYWNAMGNRYWPAFYLIDGHGRVRARYYGEMHEGDAQTRAI
                     ESVIGKLAAED"
     gene            complement(4261..4635)
                     /locus_tag="VHE37_11365"
     CDS             complement(4261..4635)
                     /locus_tag="VHE37_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36180.1"
                     /translation="MTRRLAIGDTRGDLTILEMMNMKMSNKPLSIALGAAFAVTGLAA
                     QAAVFQAADLGAGYMVADNAAPAAASSMSTGGAKKGEAKCGEKGSNCEKGAKAGECMC
                     GASKGKKADGKCGEGKCGASKK"
     gene            4717..5004
                     /locus_tag="VHE37_11370"
     CDS             4717..5004
                     /locus_tag="VHE37_11370"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HVT36181.1"
                     /translation="MPSLTVVDLLGLAAGTLTTVAFVPQVWRVWRTRSAADISLAMYV
                     VFTCGTSTWLAYGLALHALPVIVANVITTGLAGCVLLMKLRFEREKAEKNC"
     gene            5060..5135
                     /locus_tag="VHE37_11375"
     tRNA            5060..5135
                     /locus_tag="VHE37_11375"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5093..5095,aa:His,seq:gtg)
     gene            5157..5232
                     /locus_tag="VHE37_11380"
     tRNA            5157..5232
                     /locus_tag="VHE37_11380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5190..5192,aa:Lys,seq:ctt)
     gene            5299..6927
                     /locus_tag="VHE37_11385"
     CDS             5299..6927
                     /locus_tag="VHE37_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT36182.1"
                     /translation="MTSSSRILVAWLAVALVWFGTLGVRPLAHPDEGRYSEISREMLV
                     TGDWITPRLDGLKYFEKPPLQYWATAAAFAAFGQSDFVARLWTALCGFISVLAVWYTA
                     RRLWGRTAAHYAALAAAGMAYLIAISHVVTLDSAVSGFLTLALCGFLRAQHDGASAAE
                     NRRWMLVAWASMAAAMLSKGLIGVVLPGAVLLLYSALCRDWRPWARMQWLRGLALFAL
                     IAAPWHVLVAMRNPEWAHFYFVHEHFERYTTTEHHREGAWWYFIPLLLAGTLPWTSLL
                     PRALLQGWRDDAGTFRPRRFLVIWAAFIFFFFSISGSKLPGYILPVFPALALLLGPLL
                     AALPSAALRRHLATPIVFGILLLVAAPFFAHSGSERTPMELNRAATYWLVPAALLMLG
                     CCIGALRATLHNRTLSATGWLAAGSLLLWSGVMFGYNAYSPTMSSAALAQSLQGRLPA
                     SAPAYTLRLYDQTLPYYLRRTVTPVEWVDELELGEQQEPQRWIAQLPEFVQRWRDLSA
                     GLAVMPPDTYTQLQQEGVPMTLVERNARRVVVLR"
     gene            6924..7307
                     /locus_tag="VHE37_11390"
     CDS             6924..7307
                     /locus_tag="VHE37_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HVT36183.1"
                     /translation="MKLLEFGLVLAGVLLNAFAQLLLKAGTRNIGHFEFSLANVIPIG
                     WKLATNLPIIGGLSCYVVSVVVWILALSRVEVSIAYPMLSIGYVVNAALAYWLFGEAV
                     TPLRMAGIAVIIVGVCLVARSGQSA"
     gene            7304..8413
                     /locus_tag="VHE37_11395"
     CDS             7304..8413
                     /locus_tag="VHE37_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HVT36184.1"
                     /translation="MNPFLPFTRPSIDEETIAAVGDVLRSGWITSGPQVKAFEAALSA
                     RYGQRPVRVCNSATAALEMALRVAGVGEGDEVITTPLSWVATANVVIEVGATPVFADV
                     DPVTRNIDLELVERAITKKTRAIIPVDLTGLPVDRDRLYAMAQQHGLRVIEDAAQSQG
                     SNWKGREIGSFGDLVSFSFHANKNLTSAEGGCLVLNNEDEARLFEKLRLQGITRWADG
                     GMDCDVLGGKSNLTDIAAVIGLHQLKKLDGFNARRRELAKKYFERWDRTNGCDLPPAD
                     FAQSNWHMFQPLLPRGTSRGAFIEQMKERGIGVGVHYPAMHLFTYYRARGFHAGQFPH
                     AEDIGARTVTLPLFPAMADGDIDRVCAAVTESLKA"
     gene            8410..9369
                     /locus_tag="VHE37_11400"
     CDS             8410..9369
                     /locus_tag="VHE37_11400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT36185.1"
                     /translation="MSTPDLVPNLSVIIPVYNEQDSLPAMFARLYPALDALKLSYEII
                     FINDGSRDRSAALLAEQHAARPDVTRVILFNGNFGQHRAILAGFAHARGQRIVTLDAD
                     LQNPPEEIAKLLASMDQGHDYVGSIRESRNDGWFRHYASRAMNFVRERITHIQMTDQG
                     CMLRAYSRRIVDTINQCQEMNTFIPALGYSFAQNPSEVMVRFDERVAGESKYSLYKLI
                     RLNFDLMTGFSLVPLQLFSMFGMIVSAVSGIFVVILALRRLIIGPEEGGLFTLFGLAF
                     FFIGIALFGIGLLGEYIGRIYQEVRQRPRYLIAAILENKTDGA"
     gene            9491..10405
                     /locus_tag="VHE37_11405"
     CDS             9491..10405
                     /locus_tag="VHE37_11405"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HVT36186.1"
                     /translation="MNASAVVFAYHNVGVRCLQVLLAQGVDVRLVVTHEDNPAENIWF
                     SSVRRLALERGIPVITPADPNATEIVARVREANPDFLFSFYYRHMLKAPLLSIAPRGC
                     FNLHGSLLPKYRGRVPINWAIIKGETETGATLHAMTEKPDNGDIVDQMAVPILPDDTA
                     QDVFDKVLVAAELVLHRSLPALVAGTAKLTRQDLSKGAYFGGRKAEDGRIAWTQTAQQ
                     VHDLVRAVTRPYPGAFTDTAAGRLVIWRTLRTSRRDAGLPELIADDALHVRCCDGAWL
                     RVLEAELDGKPLDAARLRNEHGGKLQLV"
     gene            10418..11461
                     /locus_tag="VHE37_11410"
     CDS             10418..11461
                     /locus_tag="VHE37_11410"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HVT36187.1"
                     /translation="MKKILILGVNGFIGHHLSKRIMETTDWHVFGMDMNSERVSDLLD
                     NKRFHFFEGDITINKEWIEYHVRKCDVILPLVAIATPASYVQQPLRVFELDFEANLPI
                     VRACVKHKKRLLFPSTSEVYGMCSDSEFDPDNSPMVYGPINKPRWIYACAKQMMDRVI
                     HAYGMTEGLDYTLFRPFNWIGAGLDNIHTAKEGSSRVITQFLGHIVRGEPIKLVDGGG
                     QKRAFTYIDDGIDALMKIIENDGGKATRQIYNIGNPKNNFSVRELANMMLKLAAEYPE
                     YRDNAARVKLTETTSGAYYGEGYQDVQNRVPKIENTMKDLGWKPQVSMDAALRGIFDA
                     YRTHVADAGGLVD"
BASE COUNT         1925 a         3758 c         3828 g         1978 t
ORIGIN      
        1 ctgttcaagt cgctggcacg cgcgcagatg tcggaccaga ccaccggcat gctcaagatc
       61 ctgttccatt ccgaaacgct ggaagtgctg ggcgtgcact gcttcggcga ccgcgcgtcg
      121 gagatcgtgc acatcggcca gacggtgatg gccagcccgg ccatcaacaa cctgcgctac
      181 ttcgtcaaca ccacgttcaa ctatccgacc atggcggagg cgtatcgcgt ggcggcacac
      241 aacggcctca accgtctggc ctgaggagaa aaccgatgcg agctgcaatt ccgttcttgc
      301 tgttgatcag catcagcgct gccgccgaag acctgcgcga ggccatcgac cgcctcaccc
      361 cgcaggtgga gccgcacgtg gtggccaacc ggcgttatct ccatgaacac ccggagctgt
      421 cgaaccgcga ggtgaatacg tcgaaatacg tggcgcagaa gctgaaggcg ctgggcatcg
      481 aggtgaccac cggcgtggcg aagaccggtg tggtcggcgt gctgcgcggt ggcaagcccg
      541 gcccggtggt ggcgctgcgc gcggacatgg atgccttgcc tgtcgtcgag gaagtggacc
      601 tgccgttcaa atccacggtg cgcacttcgt tcgacggcaa ggacgtgggc gtgatgcacg
      661 cctgcggcca cgatgcgcat gtcggcatcc tgctcggcgt tgcggaaatc ctgtcgaagc
      721 tcaagggcga cttgccgggc acggtgaagt tcatcttcca gccggcggag gagggcgcgc
      781 cgctgggcga ggagggcggc gccgcgctga tggtgaagga aggcgtgatg agcaacgcgc
      841 ccaaacctga ggtgatcttc gggctgcacg tgctctccat ctatgacgtg ggccagctgg
      901 cgtaccgcgc cggtggcgcc atggccagcg cggacgattt cgtcatcgtg gtgcacggca
      961 agcagtcgca cggcgcgtac ccgtggctgg gcaccgatcc ggtcgtggtg gcgtcgcaga
     1021 tcgtgctcgg gctgcagacc attccgagcc ggcagatgga cctgaccaag gcgccggtga
     1081 tcgtcaccgt cggcaagatc gagggcggcg tgcgcaacaa catcattccc gatgcggtca
     1141 cgctcaaggg cacgttgcgc tcgctcgatt cggagatgcg caagcagctg caggagaagg
     1201 tgaagcgcac cgcggaagat atcgcagcga gcgccggcgc aacggccgag gtgcatatcg
     1261 gcgaagagac ttcgtacccg gtgacttaca acgatcccgg gctcaccgcc gccatgctgc
     1321 cgacgctgca gcgggtgggc ggcgccggtc tcaccaacgc cgcagtgcag atgggcgcgg
     1381 aggacttctc ctacttccag cagcaggtgc cggggctgta cgtgttcatc ggcatccgca
     1441 cgccgggcgc gccgaaggac gagttcgcgc ccaaccattc gccgcgcttc aagatcgacg
     1501 aaagcggcct gccgctgggc gtgcgcacgc tggccaacct caccgtggac tacatgcagc
     1561 agcacgcgcc atgaagttga ttgacctgcg cagcgacacc gtcacccgcc cgacgccggg
     1621 catgctgcag gcgatgggcg ccgctgccgt cggcgacgat gtgtggggcg acgatccaac
     1681 cgtcaacgcg ctgcaggcga aggtggcgga gcgcgcgggc aaggaagcgg ggctgttctt
     1741 tcccagcggc acgcagtgca acctcgcggc gctgatgtcg cattgcgagc gcggcgatga
     1801 gtacatcgtc ggccaggccg cgcacacgta taaatacgag ggcggcggcg cggcggtgct
     1861 cggcagcatc cagccgcaac cgatcgagaa tgcagccgac ggcacgctgc cgctggacaa
     1921 ggtcatcgcc gcgatcaagc cgccggacga tcacttcgcg cgcacgcgcc tgctcgcgct
     1981 ggagaacacc atcggcggcc aggtgctgcc ggcggcttac gtgtccgagg tcaccgcgct
     2041 ggcgcggcag cgcaagctgg ccacgcatct cgacggcgcg cgcatattca acgcggcggt
     2101 cgccagcgac tcatcgctgg cggagctctg cgcgccgttc gacacggtgt cgatctgctt
     2161 ctcgaaaggc ctcggcgcac ccgtgggctc ggtgctggtg ggcccgcgtg cgcagctcgc
     2221 gcgcgcgcgg cgctggcgca agatgctcgg cggcggcatg cgccagtcgg gctttcttgc
     2281 cgccgcgtgc ctgtacgcgc tggatcatca cgtgcagcgc ctggccgagg atcacgccaa
     2341 cgccgcgcgg ctggcacgcg gactggcgca gatcgcgcag gtgaaggtga ccgcgcagca
     2401 caccaacatg gtgttcgtgc aattcccgga agcgcattgc gccaggctcg aagcgcagct
     2461 acgcgcacag ggcatgatca tgcaggcgct gtatgcgtcg cggctggtca cgcacctgga
     2521 cgtgagcgcg gatgatgtcg accgcttcgt cgccgcggtg caagcctatt tcgcttccgc
     2581 ccagggctga ccggcagccg gcccgcgccc gcaccttgcg ccggtcaggc tgcgtagaaa
     2641 tccggcatgc cgtcgggctg gcggcggaag cgcctgtagc cccaccagta ctggtcgggg
     2701 caggtgcgca ggcaggcctc gaggccgcgg ttcatcgcgg cggtggcggc ctcgacatcg
     2761 ccgctggaaa tttccgccgg agcctcgatg tagtgcgcga tatagccgcg cgcatgcggc
     2821 aggcgctcgc cgaacatgaa caccacgggc gcgccggatt cgtgcgcgag cttggccacc
     2881 agcgtcggcg tgtgcgccag ctggcccatg aacggcacga acacgccgcg ccctggcggc
     2941 ggatcgtggt cgggcatgaa gtacacgccc catttttcgc ggagcagcac aagcgagtcc
     3001 ttgcgcacgc cagcctcggt cttgagcatg cgtccgccga agcggcaacg gccctgcagc
     3061 gacagcgcat ggatgacgcc gcgctgcggc ttgtacaggc cgtagaacgg gaaattggcc
     3121 gacatgggaa tcgccaccgc ttcgaacgcg ccctggtgca gggtgagcag gatcacgccc
     3181 ttgccctggg cgaaggcgcg ctccagcacg tcgatgccgc gccactcccg caccgcgtcc
     3241 ttcaccggct gcgcggggcc tagccagatg cggaaggttt cgacgtaggt cttgaactcg
     3301 tggcccagtg aagcgcgcgc catgcgcctg cgctcggcag gcgtgaggcc gggcatgcag
     3361 cgttcaagat tgcgcagcgc cacgcgccgg cgcgaggagt gcgtgaccca cagcaggttg
     3421 ccggcgaatg cacccagggc gtgcaggaaa gacaagggca ggtggcccag cagatagagc
     3481 agcacgcgca tgcgaatcaa ccatgggttc gcgcgagcac gcgccgccga ttatgcagca
     3541 gggcgcgggt gtccgctgca ctgcgcgtcg aaagggcgtt ctctcattca accgcaccgc
     3601 cacatcgcat gttattaaat attatattaa aataaatata gttaataact aataacacgc
     3661 aaaccggccg tgcggtgcaa gcaaaatcag tcttccgcgg ccagtttgcc gatgacggat
     3721 tcgatggcgc gggtctgcgc atcgccttca tgcatctcgc cgtagtagcg ggcgcggacc
     3781 cggccatgcc cgtcaatcag gtagaacgcg ggccagtagc ggttgcccat ggcgttccag
     3841 tacgagtagt cgttgtcgat catcaccggg tgccgcacac ccagttccgt gatcttgcgg
     3901 accacgttgt cgcgcacgta ttcctgctcc agctcgggcg tgtgcacgcc gatcacctgc
     3961 agcgcgggat ggcgcgcgcg cagcgcgttg atccagggaa tgctgcggta gcagttccag
     4021 cagtcgaatg cccagaactc gaccatcacc acgtgccccg acagttcggg ccaggtcagc
     4081 ggcctggagt tgagccagtc ctgcgcctgc atatgggtga aggcgggggc gggcggtgcg
     4141 tcgcccgccg ccgccgcgca gagcgcagtg gccaggccgg cggcgcagag aatttttcgc
     4201 atgctggaaa cgcggccggc gccgggcact ccagcgccgg ccgcgtcgat tcagctcagc
     4261 ttacttcttg ctcgcgccgc acttgccttc gccgcacttg ccgtcggcct tcttgccttt
     4321 cgaggcgccg cacatgcact cgccggcctt ggcgcccttc tcgcagttgc tgcctttctc
     4381 gccgcacttg gcttcgccct tcttggcgcc gccggtggac atgctgctgg cggcagcggg
     4441 cgcagcattg tcggccacca tgtagccggc gccgagatcg gcggcctgga acaccgcggc
     4501 ctgcgcggcc aggccggtca ccgcgaaagc ggcgcccagg gcgatcgaca gcggcttgtt
     4561 cgacatcttc atgttcatca tctccaaaat ggtcaggtcg ccgcgggtat ccccgatggc
     4621 aaggcggcgc gtcacttgcc atccacggag aggatgctag ccttcgcgcc cgctccgctc
     4681 tgccaccggc catccgcaaa acatgcccga tcgttcatgc cctcgctgac cgtggtcgac
     4741 ctgctcggtc tcgctgccgg caccctgacc acggtcgcct tcgtgccgca ggtgtggcgc
     4801 gtgtggcgca cgcgctcggc cgccgacatc tcgctggcca tgtacgtggt gttcacctgc
     4861 ggcaccagca cctggctggc ctacgggctg gcgctgcatg cgctgccggt gatcgtggcc
     4921 aatgtcatca ccaccgggct ggccggctgc gtgctgctga tgaagctgcg cttcgagcgc
     4981 gaaaaagccg aaaaaaactg ttgatcgcca gagtgttttg ttatcatgcg cgccctccgc
     5041 gggtcgcccc gcggcgatgg tggccgtagc tcagttggta gagtcccaga ttgtgattct
     5101 ggttgtcgcg ggttcgagcc ccgtcggtca ccccattttc gcaagggaag aaacacgggc
     5161 tgctagctca attggtagag cagctgactc ttaatcagta ggttcccggt tcgagtccgg
     5221 ggcagcccac caaaatgacc gaggtattcg cgcctgcgca tgacgcaggc gtttttgttt
     5281 gcaggacttg atcgatcgat gacgagcagc tcgcgcatcc tggtggcctg gctggcagtg
     5341 gcgctggtct ggttcggcac gcttggcgtg cggccgctgg cgcacccgga cgaagggcgc
     5401 tactcggaaa tctcgcgcga gatgctggtc accggcgact ggatcacgcc gcggctcgat
     5461 ggcctcaagt acttcgagaa gccgccattg cagtactggg ccacggcggc ggcgttcgcc
     5521 gctttcggcc agtcggattt cgtcgcccgc ctgtggaccg cgctgtgcgg cttcatcagc
     5581 gtgctggcgg tgtggtacac cgcgcggcgc ctgtggggcc gcactgcagc gcactacgcg
     5641 gcgctggcgg cggcgggcat ggcctatctc atcgccatca gccacgtggt cacgctcgac
     5701 tccgcggtca gcggcttcct cacgctggcg ctgtgcggat tcctgcgtgc gcagcacgac
     5761 ggcgccagcg cggcggagaa ccgccgctgg atgctggtgg cctgggcgtc gatggccgcg
     5821 gccatgctgt cgaaaggcct gatcggcgtg gtgctgcccg gcgcggtgct gctgctgtac
     5881 agcgcgctgt gccgcgactg gcggccgtgg gcgcgcatgc agtggctgcg cgggctggcg
     5941 ctgttcgcgc tgatcgccgc gccgtggcac gtgctggtgg cgatgcgcaa ccccgagtgg
     6001 gcgcatttct atttcgtcca cgaacatttc gagcgctaca ccaccaccga gcatcaccgc
     6061 gagggcgcct ggtggtattt catcccgctg ctgctggccg gcacgctgcc gtggaccagc
     6121 ctgctgccgc gcgcgctgct gcagggctgg cgcgacgatg ctggcacttt ccgcccgcgc
     6181 cgcttcctgg tgatctgggc cgcgttcatc ttcttcttct tcagcatttc cggatcgaag
     6241 ctgccgggct atatcctgcc ggtgttcccg gcactggcgc tgctgctggg cccgctgctg
     6301 gccgcgctgc cgtcggctgc gctgcgccgg cacctggcca cgcccatcgt gttcggcatc
     6361 ctgctgctgg tcgcggcgcc gttcttcgcc catagcggaa gcgagcgcac gccgatggag
     6421 ctcaaccgcg ccgcgacgta ctggctggtg cctgcggcgc tgctcatgct gggctgctgc
     6481 atcggcgcgc tgcgcgccac gctgcataac cgcacgctgt ccgccaccgg ctggctggcc
     6541 gccggctcgc tgctgctctg gagcggcgtg atgttcggct acaacgccta ttcgcccacc
     6601 atgtccagcg cggcgctggc gcaaagcctg caggggcggc tgcctgccag cgcgccggcc
     6661 tacacgctgc gactgtacga ccagacactg ccgtactatc tgcgccggac ggtgacgccg
     6721 gtggagtggg tggatgagct ggaactgggc gagcagcagg agccgcagcg ctggatcgcg
     6781 cagctgcccg agttcgtgca gcgctggcgc gatctgtccg cgggcctggc ggtgatgccg
     6841 ccggacactt acacgcagct gcagcaggag ggcgtgccga tgacgctggt ggaacgcaat
     6901 gcgcggcggg tggtggtgct gagatgaaat tgctggagtt cggactggtg ctggccggcg
     6961 tgctgctcaa tgccttcgcc cagctgctgc tcaaggccgg cacgcgcaac atcggccatt
     7021 tcgaattttc gctggccaat gtgattccca tcggctggaa gctggccacc aacctgccga
     7081 tcatcggcgg cctgtcgtgc tacgtggtca gcgtggtggt gtggatcctg gcgctgtcgc
     7141 gcgtcgaggt gagcatcgcc tacccgatgc tgtcgatcgg ctacgtggtc aacgccgcgc
     7201 tggcgtactg gctgttcggc gaggcggtga cgccgctgcg catggccggc atcgcggtga
     7261 tcatcgtcgg cgtctgcctg gtggctcgca gcgggcagag cgcatgaacc cgttccttcc
     7321 tttcacccgg ccgagcatcg acgaggagac catcgccgcg gtcggcgacg tgctgcgttc
     7381 gggatggatc accagcggcc cgcaggtgaa ggccttcgag gccgcgctgt ccgcgcgtta
     7441 tggccagcga ccggtgcgcg tctgcaactc cgccaccgcc gcgctggaga tggcgctgcg
     7501 cgtggccggc gtgggcgagg gcgacgaggt catcaccacc ccgctgtcgt gggtggccac
     7561 cgcgaatgtc gtcatcgaag tcggcgccac gccggtgttc gccgatgtcg acccggtgac
     7621 gcgcaacatc gacctggagc tggtcgagcg cgcgatcacc aagaagaccc gcgccatcat
     7681 cccggtggat ctcaccggcc tgccggtcga ccgcgatcgc ctgtacgcga tggcgcagca
     7741 gcacggcctg cgcgtgatcg aggacgcggc gcagtcgcag ggttccaact ggaaggggcg
     7801 cgagatcggc agcttcggcg acctggtttc cttcagcttc cacgccaaca agaacctgac
     7861 cagcgccgag ggcggctgcc tggtgctgaa caatgaagat gaagcaagac tatttgagaa
     7921 actccggttg cagggcataa cccgatgggc tgacggcggc atggactgcg acgtgctcgg
     7981 cggcaagtcc aaccttacgg acatcgcggc ggtgatcggc ctgcaccagt tgaagaaact
     8041 cgatggattc aacgcgcgcc ggcgcgagct ggcgaaaaaa tattttgagc gctgggaccg
     8101 caccaacggc tgcgacctgc cgccggccga tttcgcgcag agcaactggc acatgttcca
     8161 gccgctgctg ccgcgcggca cgagccgcgg tgcgttcatc gagcagatga aggagcgcgg
     8221 catcggcgtg ggcgtgcatt acccggccat gcacctgttc acctactacc gcgcgcgcgg
     8281 cttccacgcc ggccagtttc cgcatgccga ggacatcggg gcgcgcaccg tcacgctgcc
     8341 gctgttcccg gccatggccg atggagacat cgaccgcgtc tgcgccgccg tcaccgagtc
     8401 gctgaaagca tgagcacgcc cgatctggtt ccgaatctgt cggtcatcat cccggtctac
     8461 aacgagcagg attcgctgcc ggcgatgttc gcgcgcctgt acccggcgct cgatgcgctg
     8521 aagctgagtt acgagatcat cttcatcaac gacggttcgc gtgaccgttc cgcggcgctg
     8581 ctggccgagc agcacgcggc gcggcccgac gtgacgcgcg tgatcctgtt caacggcaac
     8641 ttcggccagc atcgcgccat cctcgccggc ttcgcccacg cgcgcgggca gcgcatcgtc
     8701 acgctcgacg cggacctgca gaatccgccg gaagaaatcg ccaagctgct ggccagcatg
     8761 gaccaggggc acgactacgt cggctcgatc cgcgagtcgc gcaacgacgg ctggttccgc
     8821 cactacgctt cgcgcgccat gaatttcgtg cgcgagcgca tcacgcacat ccagatgacc
     8881 gaccagggct gcatgctgcg cgcgtacagc cggcgcatcg tcgacaccat caaccagtgc
     8941 caggagatga acaccttcat tcctgcattg ggttattcgt tcgcgcagaa tccgagcgag
     9001 gtgatggtgc gcttcgacga gcgcgtggcc ggcgagagca agtattcgct gtacaagctg
     9061 atccgcctca acttcgacct gatgaccggc ttctcgctgg tgccgctgca gctgttctcc
     9121 atgttcggca tgatcgtgtc ggcggtgtcg ggcattttcg tggtgatcct cgcactgcgc
     9181 cgcctgatca tcggcccgga agagggcgga ctgttcaccc tgttcggcct ggcgttcttc
     9241 ttcatcggca tcgcgctgtt cggcatcggc ctgctgggcg aatacatcgg ccgcatctac
     9301 caggaagtgc gccagcggcc gcgctacctg atcgccgcca tcctggaaaa caagacggac
     9361 ggcgcttgag acgtttttcg agcccaaccg cactgcctgt tttgatgtta gttattattg
     9421 aatgttttaa tattcggtaa atgatttata acatcgaaaa tggccatgcg gtgaagggaa
     9481 aatacgcgcg atgaacgcca gcgcggtggt cttcgcctat cacaatgtcg gcgtgcgctg
     9541 cctgcaggtg ctgctggcgc agggcgtgga cgtgcgcctg gtggtgacgc acgaggacaa
     9601 tcccgccgag aacatctggt tttccagcgt gcggcggctg gcgctggaac gcggcatccc
     9661 ggtgatcacg ccggccgatc ccaatgctac ggaaatcgta gcaagggtgc gcgaggccaa
     9721 tcccgatttc ctgttctcgt tctattaccg ccacatgctg aaggcgccgc tgctgtcgat
     9781 cgcgccgcgt ggctgtttca acctgcacgg ctcgctgctg cccaagtacc gcggccgcgt
     9841 gccgatcaac tgggccatca tcaagggcga gaccgagacc ggcgccacgc tgcacgccat
     9901 gaccgagaag ccggacaacg gcgacatcgt cgaccagatg gcggtgccga tcctgcccga
     9961 tgacaccgcg caggacgtat tcgacaaggt gctggtggcg gccgagctgg tgctgcatcg
    10021 cagcctgccg gcgctggtgg ccggcacggc aaagctcacc aggcaggacc tgtcgaaggg
    10081 cgcgtacttc ggcggccgca aggccgagga cgggcgcatc gcctggacgc agaccgcgca
    10141 gcaggtgcac gacctggtgc gcgcggtgac gcggccgtat cccggcgcct tcaccgacac
    10201 cgctgccggc cggctggtga tctggcgtac attgcgcacc tcgcgccgcg acgccggcct
    10261 gcccgagctg atcgcggacg acgcgctgca tgtgcgctgc tgcgacggcg cctggctgcg
    10321 cgtgctggag gcggaactgg acggcaagcc gctcgacgcg gcgcgcctgc gcaacgaaca
    10381 tggcggcaag ctgcaactgg tttaacgagt ctctctcatg aaaaaaatcc tgatcctcgg
    10441 cgtcaacggc ttcatcggcc accacctgtc caagcgcatc atggagacca ccgactggca
    10501 tgtcttcggc atggacatga acagcgagcg cgtgtccgac ctgctggaca acaagcgctt
    10561 ccacttcttc gaaggcgaca tcaccatcaa caaggagtgg atcgagtacc acgtgcgcaa
    10621 gtgcgacgtg atcctgccgc tggtggccat cgccacgccg gcctcctacg tgcagcagcc
    10681 gctgcgcgtg ttcgagctgg acttcgaggc caacctgccg atcgtgcgcg cctgcgtgaa
    10741 gcacaagaag cgcctgctgt tcccgtccac ctccgaggtg tacggcatgt gcagtgacag
    10801 cgagttcgat cccgacaact cgcccatggt ctacggcccg atcaacaagc cgcgctggat
    10861 ctacgcctgc gccaagcaga tgatggaccg cgtgatccac gcctacggca tgaccgaggg
    10921 actcgactac accttgttcc gcccgttcaa ctggatcggc gccggcctgg acaacatcca
    10981 cacggcgaag gaaggcagtt cgcgcgtcat cacccagttc ctcggccaca tcgtgcgcgg
    11041 cgagccgatc aagctggtgg acggcggcgg gcagaagcgc gccttcacct acatcgacga
    11101 cggcatcgat gcgctgatga agatcatcga gaacgatggc ggcaaggcta cgcgccagat
    11161 ctacaacatc ggcaacccga aaaacaattt ctcggtgcgc gaactggcca acatgatgct
    11221 caagctggcc gccgagtatc ccgagtaccg cgacaacgcg gccagggtga agctcaccga
    11281 gaccacttcg ggcgcgtact acggcgaggg ttaccaggac gtgcagaacc gcgtgccgaa
    11341 gatcgagaac accatgaagg atctcggctg gaaaccgcag gtaagcatgg acgcggcgct
    11401 gcgcggcatc ttcgatgcct accgcacgca cgtggcggat gcgggtggcc tggttgatta
    11461 atcagtagga gcgcgccttg cgcgcgatg
//
LOCUS       DASZZC010000536         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1138210,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000536 DASZZC010000000
VERSION     DASZZC010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138210"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..493)
                     /gene="arsC"
                     /locus_tag="VHE37_11415"
     CDS             complement(110..493)
                     /gene="arsC"
                     /locus_tag="VHE37_11415"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907414.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HVT36188.1"
                     /translation="MTKSPSITLYHNPRCGRSRNTLQFIRDAGIEPQVIEYLKTPPTR
                     QQLEKLARRIQGGARRLLREKEPLAAELGLLDSSISDQRLIAAIAAHPILLNRPVVVR
                     GEEVEVCRPSETVKEFLAGGNRRAS"
     gene            complement(529..1371)
                     /locus_tag="VHE37_11420"
     CDS             complement(529..1371)
                     /locus_tag="VHE37_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVT36189.1"
                     /translation="MRKPCGPLLTLPVHQALLAARAARAASVACSLDLDRTTTEVEVS
                     AHDWAWHGQRYPYLETCKDRTIYHWTGDGFAPVSRFGSGLIKLVPTPWGAPTFEIDGI
                     KMLPTAQVSPYDDAQRKVALVRPQGQVVLDTCGGLGYFAAWCLHSGAQRVLSYEKNPD
                     VVWLRSLNPWSPDDDAALTLTQGDITEHIGDVGTASVGAILHDPPRFGIAGELYSQAF
                     YDELARVLRPGGRLFHYTGTPNKLTSRRDVPSEVAARLQRAGFTTEMNGDGVLATRRA
                     AHRK"
     gene            complement(1380..1751)
                     /locus_tag="VHE37_11425"
     CDS             complement(1380..1751)
                     /locus_tag="VHE37_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36190.1"
                     /translation="MSIAPSRIRQIEAELVSRGYAQRHWRRGAMGTRVYAKGSERVVI
                     MRNTGADRDAPGDASVEEWFERLEALLEDLRKGARISELYLCGERPVELPERFLRWCK
                     SMGVQLHAGDDDAAAPASVEE"
     gene            1840..2826
                     /locus_tag="VHE37_11430"
     CDS             1840..2826
                     /locus_tag="VHE37_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HVT36191.1"
                     /translation="MQLALAPMQGLVDDVLRDVLTRVGGIDWCVSEFVRVNDQLLPAK
                     CFRELAPELAQGARTRAGTPMRVQLLGSDPACLADNAALACTLGAPVIDLNFGCPAKT
                     VNRSRGGAVLLKEPELLHRIVREVRRTVPAEIPVSAKMRLGYETPDGALDCARAFADA
                     GASAIVVHARTKLDGYKPPAHWEWVARVRDAVSVQVYANGEIWNVDDWRRCRAVSGVD
                     DIMLGRGLVSQPDLARQIVDAREGRDVAPMSWAELLPVLQDFWLQVRAKLTPLQAPGR
                     VKQWLALLTRNYAEATQLFSVVRTEHDCARIGALLGLPAMSTDDTASERMAA"
     gene            complement(2842..>3124)
                     /locus_tag="VHE37_11435"
     CDS             complement(2842..>3124)
                     /locus_tag="VHE37_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HVT36192.1"
                     /translation="SVRRRRGRADAEVAATTPGGDALFDRLRALRKRLADEQGVPPYV
                     VFADAALRHMATEQPRTVSAFAEIPGVGKYKLERYAEVFTAEIRAAVAA"
BASE COUNT          462 a         1072 c         1092 g          498 t
ORIGIN      
        1 cgcggctcac gctgatgccg ggtgtcggcc atttctgcca gctgagcgat ccgcaactct
       61 ggctggacag cgtcggcgct ttctaccgcg aggaagccgg gcgcagcacc taggaagcgc
      121 gccggttccc gccggcgaga aactccttga cggtttccga aggcctgcac acttccacct
      181 cctcgccacg gaccaccacc ggccggttga gcaggatcgg atgcgcggca attgccgcga
      241 tcaggcgctg atcggagatg gatgaatcga gcagccccag ctccgccgcc agcggttcct
      301 tttcgcgcag caggcggcgc gcgccgccct ggatgcgccg ggcaagcttt tccagctgct
      361 ggcgcgtggg cggcgttttc aggtactcga tgacctgcgg ctcgatgccg gcgtcgcgga
      421 tgaactgcag cgtgttgcgc gacctgccgc acctggggtt gtggtaaagc gtgatggacg
      481 gggatttggt catgtgggat cgcggtgtgt ggagcgcagc tggcgcacct attttcgatg
      541 ggcggcgcgc cgcgtggcca gcacgccatc gccgttcatc tcggtggtga aaccggcgcg
      601 ctgcaggcgc gcggcgactt cgctgggcac atcgcgccgg ctggtgagct tgttcggcgt
      661 gccggtgtag tggaacagcc ggccgccggg cctgagcacg cgcgccagct cgtcgtagaa
      721 cgcttgcgag tacaactcac cggcaatgcc gaagcgcggc ggatcatgca ggatggcgcc
      781 gacagacgca gtgcccacgt cgccgatgtg ttcggtaatg tcgccctgcg tcagcgtcag
      841 cgccgcgtca tcgtccggcg accatggatt gaggctgcgc agccagacca cgtcgggatt
      901 cttttcatac gacagcacgc gctgcgcgcc gctgtgcagg caccacgcgg cgaagtagcc
      961 gagcccgccg caggtatcca gcaccacctg cccctgcggg cgcaccagcg ccaccttgcg
     1021 ctgcgcgtcg tcatacggcg agacctgcgc cgtgggcagc atcttgatgc cgtcgatctc
     1081 gaacgtcggc gcgccccatg gcgtgggcac cagcttgatc agcccgctgc cgaagcgtga
     1141 gaccggcgca aagccatcgc cggtccagtg gtagatcgtg cggtccttgc aggtctccag
     1201 gtacggatag cgctggccgt gccacgccca gtcgtgtgca ctcacctcga cctcggtggt
     1261 ggtgcgatcc aggtccagcg aacacgccac gctcgcggcg cgggccgcgc gcgcagcgag
     1321 caatgcctgg tgtaccggca aggtgagcag agggccgcaa ggtttgcgca cgatgtccgc
     1381 tattcctcga cggaggcagg tgctgccgcg tcgtcgtcgc cggcatgcag ttgcacgccc
     1441 atggatttgc accagcgcag gaagcgctcg ggcaattcca ccggccgttc gccgcacagg
     1501 tacagctcgc ttatgcgcgc gcccttgcgc agatcttcaa gcaacgcctc cagccgctcg
     1561 aaccactctt ccacgctggc gtccccgggc gcatcgcgat cggcgccggt gttgcgcatg
     1621 atcaccacgc gctcgctgcc cttggcgtac acgcgtgtgc ccatggcgcc gcggcgccaa
     1681 tggcgctgcg cgtatccgcg cgaaaccagc tcggcctcga tctggcggat gcgggagggt
     1741 gcgatgctca tggcgggctc cggtggacgc gcagcttaac cggccggccg cgccggcggc
     1801 gggtaaaatg cgcgcggttt ccacttccgc attttctcca tgcagcttgc tctcgccccg
     1861 atgcagggcc tggtcgacga tgtcctgcgc gacgtgctca cgcgcgtggg cggcatcgac
     1921 tggtgcgtga gcgagttcgt gcgcgtcaac gaccagctgc tgccggcgaa gtgcttccgc
     1981 gaactggcgc ccgagctcgc acagggcgcg cgcacgcgcg cgggcacgcc catgcgcgtg
     2041 cagctgctcg gctccgatcc cgcgtgtctc gccgacaacg cggcgctggc ctgcacgctc
     2101 ggcgcgccgg tcatcgacct caacttcggc tgcccggcca agacggtgaa ccgctcacgc
     2161 ggcggcgcgg tgctgctgaa ggagcccgag ctgctgcatc gcatcgtgcg cgaggtgcgg
     2221 cgcacggtgc cggcggaaat tccggtcagc gcgaaaatgc gcctgggcta cgagacaccc
     2281 gacggcgctc tcgactgcgc gcgcgccttc gccgatgccg gcgcgtccgc gatcgtggtt
     2341 cacgcgcgga ccaagctcga tggctacaag ccgcccgcgc actgggagtg ggtggcgcgc
     2401 gtgcgcgacg cggtgagcgt gcaggtttac gccaacggcg aaatctggaa cgtggacgac
     2461 tggcgccgct gccgcgcggt gagcggcgtg gacgacatca tgctcggccg cgggctggtg
     2521 tcgcagccgg acctggcgcg ccagatcgtc gatgcacgcg aagggcgcga cgtggcgccg
     2581 atgagctggg ccgagctgct gccggtgctg caggatttct ggctgcaggt gcgcgccaag
     2641 ctcacgccgc tgcaggcgcc ggggcgggtc aagcaatggc tggcgctgct gacgcgcaac
     2701 tacgccgagg cgacgcaatt gttttccgtc gtgcgcaccg agcatgactg tgcgcgcatc
     2761 ggcgcgctgc tgggactgcc cgcaatgtcc acggacgaca ccgcaagcga acgcatggcg
     2821 gcctgagccg cgcgcacgac ctcaggcggc aacggccgcg cggatttccg cggtgaacac
     2881 ctcggcgtaa cgctccagct tgtacttgcc cacgcccgga atttccgcga aggcgctcac
     2941 cgtgcgcggc tgctcggtgg ccatgtggcg caatgcggcg tcggcgaaca ccacgtaagg
     3001 cggcacgccc tgctcatccg ccagccgctt gcgcagcgcg cgcaggcggt cgaacagcgc
     3061 gtcgcccccc ggcgtggtcg cggccacctc ggcatctgcg cggccacggc ggcggcgaac
     3121 ggat
//
LOCUS       DASZZC010000537         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_557734,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000537 DASZZC010000000
VERSION     DASZZC010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557734"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..858)
                     /locus_tag="VHE37_11440"
     CDS             complement(211..858)
                     /locus_tag="VHE37_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT36193.1"
                     /translation="MPIRSKQHMDDVKEGIMHALARCITHKGIADVTIADLIKESGLS
                     AGAIYRHFENKDELLVGVVRHRIKALDEKVVLQELGSFDFWKFVDWTLQRVTTTEHVY
                     LAELEFVSLARVNAKIRGIYIASEKAWEGVLKKCIATLPGAANLMRRADVVQLLIDNL
                     RAIGNQIILRRMIGLKLDAQNYRRQVEMTVAGAFALAGQPAAAPRKAAVRKAVAA"
     gene            1137..2732
                     /locus_tag="VHE37_11445"
     CDS             1137..2732
                     /locus_tag="VHE37_11445"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HVT36194.1"
                     /translation="MLAGALQVGTAAAADMPAAPDPLRAACDTLKFADFAALKDAPTR
                     VMDSGVAEAAGEVPASCLLRGYVSPQVGFEMKLPLSGWNGKLAEIGSGGYAGSTQVGG
                     DKQMCDELVRRGYACVHSDYGHTSGITEKSPAALDAVWAYNNLPALVDYGYRGIRVVG
                     VAAKAIVKSFYGSAQKRAYFIGCSNGGRQAMVAAQRFPDDYDGILAMEPAINLAGTFL
                     NFVYNHKAVTDDEGKPLFKPSDLELLHNRVIAQCDLDDGIKDGVVGNPSACAFDPATL
                     ACGAGKSENCLNPAQVAAAKKVYAGAMTSSGRRFYRGHTMPGAERGSFGFAVTRPLAE
                     YALKDFVRYLAFMPSPGPTAKLSDVDFDEDYKRLPLMEALYQSDNPDLRGLKEAGGKL
                     IMVQGWDDSGTPFPLNTIDYYETMSRTMGGLKATQQFARLYMVPGRQHCVGGEGADSA
                     DFIGQLESWVERNRAPDMIKAYHLKPTDNMMDHLREPDDPARVEFSRPLYPYPLQAKY
                     QGSGDPNDYRSFKPVELSTIHLK"
     gene            2738..>3073
                     /locus_tag="VHE37_11450"
     CDS             2738..>3073
                     /locus_tag="VHE37_11450"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HVT36195.1"
                     /translation="MSRRTAALLLAGCALGAGSAHAGPTLEQQFAQPGASARPRVWWH
                     WIDGNVTREGITRDLEWMQRSGIAGFQMFDVGRGFPALVEEPAPYLSPHWKDLLHHAA
                     SEADRLHLEM"
BASE COUNT          561 a          997 c          992 g          523 t
ORIGIN      
        1 taattaatac gatatttaat aacatcaaat ataggtgtgc ggtgagaggt tttgcggctc
       61 catcgaacgt ggaacgggtg aaccttcaat tgggattcac gggtttcatc gtcagctctc
      121 tctaacaggg ttgatgatga aactgccaac gcagagcact gaaacgctcg cgacgctcgc
      181 gcaccccggg gagagggaac cgtcggagga ctacgccgcc acggccttgc gcacagccgc
      241 cttgcgcggc gccgccgccg gctgcccggc gagtgcaaac gcgccagcca ccgtcatctc
      301 cacctgccgg cggtagttct gcgcatcgag cttcagcccg atcatgcggc gcaggatgat
      361 ctggttgccg atggcgcgca ggttgtcgat cagcagttgc accacgtccg cacggcgcat
      421 caggttggcg gcaccgggca gggtagcgat gcatttcttc agcacgccct cccaggcctt
      481 ttccgaggcg atatagatgc cgcggatctt ggcgttgacc cgcgccagcg agacgaattc
      541 cagctcggcc agataaacgt gctcggtggt ggtgacgcgc tgcagcgtcc agtcgacgaa
      601 cttccagaag tcgaaactgc ccagttcctg cagcaccacc ttctcgtcca gcgccttgat
      661 gcggtggcgc accacgccga ccagcagttc gtccttgttc tcgaaatggc ggtagatggc
      721 gccggccgac aggccggatt ccttgatcag gtcggcgatg gtcacgtcgg cgatgccctt
      781 gtgcgtgatg cagcgggcga gcgcatgcat gatgccttcc ttcacatcat ccatgtgctg
      841 cttggatctg atcggcatcg tcctggcccc ggcgttctgc ggcgattcta gcggcaaatg
      901 gaacgtccgt tttgcccgcc gggcggactc cgcagctggc gcaaaaattg atttggaacg
      961 aatattcgtt ttaagatggc atcgctgccg gatcgagatg caatcatgcg ccgtccgggc
     1021 aggccggcac accgatggag gagagcgtgg accacgggca attccaggtt gcggcgcgag
     1081 gccgcgttgt aaatgcgtcg cgccccattg cggcgaaaac ggcagcaacc ctgctgatgc
     1141 tggcgggcgc tctgcaggtc ggtacggcag ctgccgccga catgccggca gcgcccgatc
     1201 cgctgcgcgc ggcctgcgac acgctcaagt tcgccgactt cgccgcgctg aaggatgcgc
     1261 cgacgcgggt gatggacagc ggcgtggccg aagccgccgg cgaagttccc gccagctgcc
     1321 tgctgcgcgg ttacgtgtcg ccgcaggtcg gtttcgagat gaagctgccg ctgtccggct
     1381 ggaacggcaa gctggcggag atcggcagcg gcggctacgc cggctccacc caggtgggcg
     1441 gcgacaagca gatgtgcgac gaactggtgc gccgcggcta cgcctgcgtg cattccgatt
     1501 acggccatac ctccggcatc accgagaaat cgccggctgc gctcgacgca gtgtgggcct
     1561 acaacaacct gcccgcgctg gtcgactacg gctaccgcgg catccgcgtg gtcggcgtcg
     1621 cggccaaggc catcgtcaag agcttctacg gcagcgcgca gaaacgcgcc tatttcatcg
     1681 gctgctccaa cggcggccgc caggcaatgg tggcggcgca gcgctttccc gacgactacg
     1741 acggcatcct ggcgatggag ccggcgatca acctggctgg caccttcctc aacttcgtct
     1801 acaaccacaa ggccgtcacc gatgatgagg gcaagccgct gttcaagccg tcggacctgg
     1861 agctgctgca caaccgcgta atcgcccagt gcgacctgga tgacggcatc aaggacggcg
     1921 tggtcggcaa tccgtcggcc tgcgccttcg atccggcgac gctggcctgc ggcgcgggca
     1981 agagcgagaa ctgcctgaat ccggcgcagg tggcggcggc gaagaaggtc tatgccggcg
     2041 ccatgacttc cagtggcagg cgcttctacc gtggacacac catgcccggc gccgaacgcg
     2101 gcagcttcgg cttcgccgtc acccgtccgc tggcggaata cgcgctgaag gatttcgtgc
     2161 ggtacctcgc cttcatgccg agccccggac ccaccgcaaa attatccgat gtcgatttcg
     2221 acgaggacta caagcgcctg ccgctgatgg aagcgctgta ccaatcggac aacccggacc
     2281 tgcgcgggct gaaggaagcc ggcggcaaac tcatcatggt gcagggctgg gacgacagcg
     2341 gcacgccgtt cccgctcaac accatcgact actacgaaac catgtcgcgc accatgggcg
     2401 ggctcaaggc gacgcagcaa ttcgcgcgcc tgtacatggt gccgggccgc cagcactgtg
     2461 tcggcggcga gggcgcggat tccgcggact tcatcggcca gctcgagtcc tgggtggagc
     2521 ggaaccgcgc accggacatg atcaaggcgt atcacctgaa gcccaccgac aacatgatgg
     2581 atcacctgcg cgagcccgat gatcccgcca gggtggagtt ctcgcggccg ctctatccat
     2641 atccgctgca ggcgaagtac cagggcagcg gcgatccgaa cgactaccgc agcttcaagc
     2701 cggtggaact gtccaccatc cacctgaagt gaggccgatg agccgccgga ctgctgcatt
     2761 gctgctggcc ggatgtgcac tcggcgccgg tagcgcgcac gccgggccaa ccctcgaaca
     2821 gcagttcgcg cagcccggcg cgtccgcgcg tccgcgcgtg tggtggcact ggatcgacgg
     2881 caacgtcacc cgcgagggca tcacgcgcga tctcgagtgg atgcagcgca gcggcattgc
     2941 cggtttccag atgttcgacg tcggccgcgg ttttccggcg ctggtggaag agccggcgcc
     3001 atacctgtcg ccgcactgga aagacctgct gcatcacgcc gccagcgaag cggaccgcct
     3061 gcacctggag atg
//
LOCUS       DASZZC010000538         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_682631,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000538 DASZZC010000000
VERSION     DASZZC010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682631"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..811)
                     /locus_tag="VHE37_11455"
     CDS             complement(116..811)
                     /locus_tag="VHE37_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT36196.1"
                     /translation="MKTSLKISIAGFVIGSLLAGCADDPNRDTERGAAIGALAGAIIG
                     NQSHSSKGKYVGAIIGGLAGAGVGHYMDKQRQDLEAKLKAEEQAKMLEITKLSDDSLK
                     IGIASDASFDVGSAQLKPEALMTYGKIAQILTSYDQTVIHIVGHTDATGSDQLNQSLS
                     ESRAASVATYMESTGLSAQRIRTEGRGKREPIASNDTADGRSKNRRVDIVLKAIVQGH
                     EQDAWTPPPYLGS"
     gene            complement(808..>2963)
                     /gene="rnr"
                     /locus_tag="VHE37_11460"
     CDS             complement(808..>2963)
                     /gene="rnr"
                     /locus_tag="VHE37_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HVT36197.1"
                     /translation="GPLTFDELIAALVMAPNEPVEALRKRLDAMVRDGQIVQNRKGAY
                     GPVSRMNLIAGEVQAHRDGFGFLIPDAGGPDIFLGPRQMRSLMNGDRVLVRIVGTDQR
                     GRPEGTLAEVIERASRDVVGRLHIDQGVSYVIPDNPRVQQDVLIPPDQRAGARHGQMV
                     VAELTALPGSRTLPVGRIKEVLGDHLAPGMEIVAAIRAHNLPHEWPAAVEREAEAIPA
                     TVQPEQMQGREDVRHLPLVTIDGADARDFDDAVHARPDKHGWTLWVAIADVSAYVRPG
                     APLDVEATKRGNSVYFPRQVIPMLPEKLSNGLCSINPEVDRLCMVCEMRVGTDGEVEK
                     SRFYEGVMRSQARLIYEDVADVLEPAASARGTRPVAAALMPHLHALRDVYQALFRARE
                     KRGAIDFDTTETRIVFSGERKIERIEPIRRNIAHRLIEECMVAANVQAAMLAERCKVP
                     TLYRVHERPDPLKVAALREFLSGRGIVLGGGEEPRALDYARALAKCKDRPDAALIQGV
                     MLRSMMQAQYNPQNTGHYGLALEHYAHFTSPIRRYPDLLLHRALKHALKKKKGTPFEY
                     SAEQMAALGTHCSMTERRADEATREVANWLKCEFMSHRVGEEFDGVVSGVTSFGLFVM
                     LDDLYIDGLVHVSSLKNDYYEFDAKRQRLVGSRTRMVYALGERVHVRVTRVSLDERKI
                     DLELTGSKSHSTSDQRGTPASRAPHGKKPSRRDRR"
BASE COUNT          435 a          994 c          991 g          543 t
ORIGIN      
        1 taatcatata attaacaaaa tgaataatat tttataacac gcaaaatgcc ggtgcggtga
       61 ccatgaaaaa gggccgtgaa cggcgtgttc acggccccgt tcactcagcg ggcgatcacg
      121 aacccaggta cggcggcggc gtccaggcgt cctgctcgtg accctgcacg atggccttga
      181 gcacgatgtc gacgcggcgg ttcttcgagc ggccgtcggc ggtgtcgttc gacgcgatgg
      241 gctcgcgctt gccgcgcccc tcggtgcgga tgcgctgagc cgacaggccg gtgctctcca
      301 tgtacgtcgc caccgaggcc gcgcggcttt ccgacaggct ctggttgagc tggtcgctgc
      361 cggtggcgtc ggtgtggccg acgatgtgga tcaccgtctg gtcgtagctg gtgaggatct
      421 gcgcgatctt gccgtaggtc atcagcgcct ccggcttgag ctgcgcgctg cccacgtcga
      481 agctggcgtc gctggcgatg ccgatcttca gcgaatcatc cgacagcttg gtgatctcaa
      541 gcatcttggc ctgttcctcg gctttgagct tggcctcgag atcctggcgc tgcttgtcca
      601 tgtagtggcc gacgccggcg ccggccaggc cgccgatgat ggcgcccacg tacttgccct
      661 tgctgctgtg cgactggttg ccgatgatcg ctccggccag cgcgccgatg gccgcgccgc
      721 gctcggtgtc gcggttgggg tcgtcggcgc aaccggcgag caggctgccg atgacgaatc
      781 ctgcgatgga aatcttgagg ctggtcttca tcttctatcc cttcgtgatg gtttcttgcc
      841 gtgcggcgcg cgcgatgccg gcgtgccgcg ctgatccgac gttgaatgag acttgctgcc
      901 ggtcagctcg agatcaattt tacgctcatc caggctgacg cgggtcacgc gcacgtgcac
      961 gcgctcgccg agcgcataga ccatgcgcgt gcggctgccg accaggcgct ggcgcttggc
     1021 gtcgaactcg tagtagtcgt tcttcagcga cgacacgtgc accaggccgt cgatgtagag
     1081 gtcgtcgagc atgacgaaca ggccgaagct ggtgacgccg ctgaccacgc cgtcgaactc
     1141 ctcgcccacg cgatggctca tgaactcgca cttgagccag ttggccacct cgcgcgtagc
     1201 ctcgtccgcg cgccgctcgg tcatcgagca atgcgtgccc agcgcggcca tctgctcggc
     1261 ggaatattcg aacggcgtgc ccttcttttt cttcagcgcg tgcttgagcg cgcggtgcag
     1321 cagcaggtcg ggatagcggc ggatcggcga ggtgaagtgc gcgtaatgct ccagcgccag
     1381 gccgtagtgg ccggtgttct gcgggttgta ctgcgcctgc atcatcgagc gcagcatcac
     1441 gccctggatc agcgcggcgt cgggacggtc cttgcacttc gccagcgcgc gcgcgtaatc
     1501 gagcgcgcgc ggctcctcgc cgccgccgag cacgatgccg cggccggaga ggaattcgcg
     1561 cagcgccgcc accttcagcg gatcgggccg ctcgtgcacg cggtacagcg tcggcacctt
     1621 gcagcgctcg gccagcatcg ccgcctgcac gttggcggcg accatgcatt cctcgatcaa
     1681 ccggtgcgcg atgttgcgcc ggatgggctc gatgcgctcg atcttgcgtt cgccggagaa
     1741 gacgatgcgc gtctcggtgg tgtcgaaatc gatggcgccg cgtttttcgc gtgccctgaa
     1801 cagcgcctgg tagacatcgc gcagcgcgtg caggtgcggc atcagcgccg ctgcaaccgg
     1861 cctggtgccg cgggcgctgg ccgcaggctc cagcacgtca gccacgtcct cgtagatcag
     1921 ccgcgcctgg gagcgcatca cgccctcgta gaagcgcgac ttctccacct cgccatccgt
     1981 ccccacgcgc atctcgcaca ccatgcacag ccggtcgacc tcgggattga tcgaacacag
     2041 gccgttggac agtttctccg ggagcatcgg gatgacctgc cgcgggaaat acaccgagtt
     2101 gccgcgcttg gtcgcttcca cgtccagcgg cgcgccgggc ctgacgtaag cggaaacatc
     2161 ggcgatcgcc acccataacg tccagccatg cttgtcgggg cgcgcgtgta cggcgtcgtc
     2221 gaagtcgcgc gcgtcggcac cgtcgatggt caccagcggc aggtggcgga cgtcttcgcg
     2281 tccctgcatc tgttccggct gcaccgtggc gggaatcgcc tcggcctcgc gctcgaccgc
     2341 cgcgggccat tcgtgcggca ggttgtgcgc gcggatggcg gcaacgatct ccatgcccgg
     2401 cgccaggtga tcgccgagca cttccttgat acgccccacc ggcagcgtgc ggctgcccgg
     2461 cagcgcagtg agttccgcca ccaccatctg gccgtggcgc gcaccggcgc gctggtccgg
     2521 cgggataagc acatcctgct gcacgcgtgg attgtctgga atgacgtagc tgacgccctg
     2581 gtcgatgtgc aggcgcccga ccacgtcgcg gctggcgcgc tcgatcacct cggccagcgt
     2641 gccttcgggc cgcccgcgct ggtcggtgcc gacgatgcgc accagcacgc ggtcgccgtt
     2701 catcagcgag cgcatctggc gtggaccgag gaagatgtcc ggtccgccgg catcggggat
     2761 caggaagccg aagccgtcgc ggtgcgcctg cacttcgccg gcgatcaggt tcatgcgcga
     2821 caccgggccg taggcgccct tgcggttctg cacgatctgg ccgtcgcgca ccatggcgtc
     2881 gagccgcttg cgcagcgcct cgaccggctc gttcggcgcc atcaccagcg cggcgatcag
     2941 ctcgtcgaac gtcagcgggc cgg
//
LOCUS       DASZZC010000539        13962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_868918,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000539 DASZZC010000000
VERSION     DASZZC010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868918"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2061)
                     /locus_tag="VHE37_11465"
     CDS             complement(<1..2061)
                     /locus_tag="VHE37_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVT36198.1"
                     /translation="MSKNFDVLGLVAADLEPTLARALLRGGATAILDLEQVRDEAAAM
                     RNFAGLLSSQHGALGLRVTPARSALARDLLAAAGARPMVVIACGDFASVAEQLAPTAQ
                     VRMLAEITAASQLANVPASFQGLVALGHEGGGWVGEDTSFILLQKLAGKTSLPVYARG
                     SVGVHSAAACRVAGAAGVVLDEQLLLLDESPLPEPLKSELARLNGAECRLFGELLEAP
                     CRAYARAGSAVLKAAEEDLRQAEGGQLPLAEWKARLASRMGWNGDGSQLLPIGQGIGL
                     AAAYRTQFGSAAKLIRAVRRAAMKQVELAAQQQALGENAPLAASHGTRYPLVQGPMTR
                     VSDSADFAAAVARGGALPLLALALMRGPQVARMLADTKARIGSLPWGVGMLGFVPHAL
                     REEQCAEIWKCKPDFALIAGGRPDQAAEFEKRGITTYIHAPAPALLRLYLEQGARRFV
                     FEGRECGGHVGPLASFALWEQMIEVLLAEVKPGEESQVHVLFAGGIHDALSAAMVAAM
                     TAPLTARGIKVGALMGTAYLFTEEIVSSGAVVAGFQKQALGCERTMNLETGPGHASRC
                     VDTRFAHEFYETRRRLIGEGKSAEEIRDTLEDLNLGRLRIASKGVNRDADGKIVDVAP
                     PEQLAAGMYMIGQVATLRHATQTIEQLHDHVCRGAAEHLSQLLEPQIGAPKPSDIAIV
                     GIGTL"
     gene            complement(2089..2904)
                     /locus_tag="VHE37_11470"
     CDS             complement(2089..2904)
                     /locus_tag="VHE37_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVT36199.1"
                     /translation="MSKNLVFIVLDSCRYDSYAAARRPNMDRLGMGLKRYSYASWTSP
                     SHYTYLMGMIPHESPQGVFASEVYKKEFTRWVDRLGVPDLSFKSFVPQLSLPHVLQEN
                     GYRTVAKVSLPVLNPFSHLNKHFDDYKLMASHNDFAGMVKEIEFDDSEPSFHFLNIGE
                     THYPYMLDPKSLPHISGVHGVFKRMDDELGQSTADKFFDDTQMKDLHRQQIKTVEYVD
                     GVLDALISKAPVGTHFIITADHGECFGENGYFGHGPIVHEKVLEVPFLEGVKV"
     gene            3148..3867
                     /locus_tag="VHE37_11475"
     CDS             3148..3867
                     /locus_tag="VHE37_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36200.1"
                     /translation="MGTRERRQREVAEREKRFVDRARELIRVEGLLNLQMSRVADGCD
                     YAVGTLYQHFSSKEDLLLAVTTETAREHVDLFRRVASWNANTRDRMFAIGVADMIFVQ
                     RNPDYFSTVQYALCEVVWGAASQERRDEFLEVNQPITQLVIGIVDDAVAKGDLQLVGF
                     TPQQLTVGLWALCLGMHNLVHAEGVLRDFDINQPYRAMCRHMQYMLNGFGWKPLSDPA
                     DEAATERLIQRIKSEVFHDLA"
     gene            3854..5197
                     /locus_tag="VHE37_11480"
     CDS             3854..5197
                     /locus_tag="VHE37_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HVT36201.1"
                     /translation="MTWREWVALAMLALAAAPAAQAMTLSEAYALALQHDPAVAESLG
                     QYKAEREPGDQERSTLYPNVSVAAGAAYDRTDSTGVFGHSNDTYPSYSATLKLRQPLF
                     RLDWFARGARADARDASADASLAQRKLDLLQQVAQRYFDVLTAQDGLAQTQAEARAVR
                     ESFDATRKRYEVELAPGTDLKEAQARNDLAGASMLAARRTLEAALDALDESTGNGRAA
                     LPRLPAELRFPPLLPADAESWIASGREHSPKLLLAQQQLAIARADLTSRKSEATPTVD
                     LVGSYGRDDTSRYVFGQKVDDGRVGLELNIPLYAGGYNASRVREAQGRLEQAQSALAR
                     TQADLERDIRARLRDVQLSMAQIDAYEKQLGSAQVAQQATANGYEAGTRTISDVLDAK
                     SRVVQAQRDLNQSRYSVLLNLLLLKQAVGVLSEKDFAEIDRLLAQGTAASTEQSP"
     gene            5194..6354
                     /locus_tag="VHE37_11485"
     CDS             5194..6354
                     /locus_tag="VHE37_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT36202.1"
                     /translation="MTKRMIIMMAIAAVVFGGVFFMSHNMSVGMADYLEHMPSPAAAI
                     STTKVSTMTWDNTVDAAATLVAAQSTTVTTEAGGIVQAIHFESGAHVQQGALLVTLDS
                     ANEQAELKRLQAQAELARLNHARREKLYGLEAISKSDLDSAESEAAAAQAAVDAQQAK
                     LAQKQIRAPFAGQLGIRQVNVGQFLDAGKPIVTLQALDPVYADFNLPEQQLGVVTAGL
                     AVEAEVAAWPGKKFGGKVVAVEPRVDEATRNFHVRAQMDNGEGKLAGGQSAHITLKLP
                     GARTLVVVPHTAISYSSYGSSVFVVGKMAHPADKDAPRMPQAGPWTDLEVTQRFVKTG
                     EERGDFVAVTDGLKVGEEIATSGLLKLRNQQPVLINNDAPPKPELNPSVPQG"
     gene            6379..9471
                     /locus_tag="VHE37_11490"
     CDS             6379..9471
                     /locus_tag="VHE37_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT36203.1"
                     /translation="MHFTDIFIKRPVLATVISLVIFLLGLRAFHDLRVREYPELQNAQ
                     VTVSVAYPGADPALMEGFITTPLEREIATADGIDFLSSSTGQGAATITANLRLDKNPN
                     LALTEISAKVNKVRGQLPQDAEDPVIEVSEGGGTAAMYLTFHSKVLDNSQITDYLTRV
                     VQPQLSSIPGVEKAQIMGERTYALRVWLKPEALAAHNMTASDVYQRLRAQNVLSAVGE
                     IKGNYVRIGLAAQTDLRSPDEFRQLVVKTEGSSLVRLADIADVALGAETYDGAAGWDG
                     DPALFVGIDIRPDANLLEVIERVRQVWPDILAQLPQGLEGTIGYDSTVYVRDAIKEVK
                     STLIEAVAIVIIVIFLFLGSMRNSLIPAVTVPLSLVGALFLMLAMGFTINLLTLLAMV
                     LAIGMVVDDAIIVMENIHRHIEQGMTPLQASFQGARELVGPIIAMTITLVAVYAPIGF
                     LTGITGKLFTEFAFTLAGAVLISGVLALTLTPMMCARILAPHHAAGGQGGLADWLDAR
                     FEGWRNGYKQRLHSALDTVHVIGAFGVIVLVSCFFLFKQLPNELAPEEDRGFVFAINE
                     TDGYSSQEYLDANFHLAQKAVLQDKDVPHIFTFSLGDSGGTSQAFMGMILRPWSERRR
                     SSTQIINELQPRLAEISALRAQIIQPPPLPTPGQGYPLEFVLKATASPEEIARVSDQI
                     VARAQASHKFFYIATRLRIDRPQTVIEVDRDKAALLGVDMSALSADLSALLAGGEVNR
                     FSYQSRSYRVIQQVARADRLNASQLEGFYTRAASGQLVPISTLVTLRQRTIARSIEHA
                     QQLNADTLVAVPAGGVTQGEGLKVFEDLAKELAPRGYQVDYSGPSRQFKQEGSALMAT
                     FAFALIVIYLVLSAQFESFRDPLIMLVTVPMSICGALLFLKIFSMTNDMMLTHISGMT
                     LNIYTQVGLVTLIGVISKHGILIVEFANKLQEQGVAKREAIEQAASIRLRPVLMTTAA
                     LVVSMIPLLAASGPGAAARFSMGLVIASGMGIGTLFTLYVVPAMYLYIGRDHAQARAK
                     "
     gene            9704..10492
                     /locus_tag="VHE37_11495"
     CDS             9704..10492
                     /locus_tag="VHE37_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36204.1"
                     /translation="MKMKWLWACVLLVFALHAQARVGQVHRCTDAKGAMVLTDQGCEQ
                     DAQITAHRPFVQPQEVEPTPLPSSLQTAAPPTIRNAPQPAAVPAAHSPDGADRAAAAA
                     VRRASGKSAAGNSDEDSSAAPADYSQPADDEPEMSQGTRLMLWSGLLLGFGGYFSLVV
                     VAFQQSKTWGVLVLLFSPLAAVIFKITHKGKGRIPSIIGSIGMALAARAVALPSTDLH
                     PMADQRPQVSATAPAASGQHGREQEADCARTASMAGVGCATGLE"
     gene            complement(10508..11356)
                     /locus_tag="VHE37_11500"
     CDS             complement(10508..11356)
                     /locus_tag="VHE37_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36205.1"
                     /translation="MSAESTQLMQQLWTAPTVYRSDLFKDQVALVSGGGSGIGRAVAL
                     LYARLGARVVICGRTQEKLDTVAAFAHGHGARMSAYACNVREAEQVDELFRKIHTEHG
                     RLDHLVNNAGGQFPQHSIDYAPKGWAAVINNNLNGTWYMMQRAAQYWREQKTPGAICN
                     IVVVTERGMPGVAHTVAARAGIIGATRTVAVEWAPLQVRVNCVAPGLTATEGLEVYPP
                     EAQAEFPRANPMKRPGTPMEIAESVIYLNGPSGSFITGEVLTVDGGGKLWGELWTAGR
                     PDWFRY"
     gene            complement(11353..12543)
                     /locus_tag="VHE37_11505"
     CDS             complement(11353..12543)
                     /locus_tag="VHE37_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT36206.1"
                     /translation="MSKLSVMQAFSGLSSDEMDILAQADRFAQKEIYPLAQRMDDEEW
                     WPTDIFPKIGETGYFGVTAPEQYGGAGMDLMTSGLILQAFGRWNHALALAWVAHENLC
                     LHNILRNANEEQKKKYLPGLCKGKLIGALGLTEPGAGSDALGSMRTTARREGGHYILN
                     GRKIYITNGPVADVLLVYAKTDKDKGAQGISAFIVEKDFPGFKVAQKLIKMGFRGSQT
                     AELVFDECRVPAENLVGEENRGVKVVMSGLDLERAMISPICLGIAERALTLSLDYARQ
                     RKQFGQAISSFQMIQDKLAQMYVWVESMRLFTYQVLRAAATISEDEGGRGDIHKLTAA
                     GVMYVAETMNQVLNEAVQVHGGSGYIWESEINRLYRSIKLLEIGAGTTEVRKIIIAGE
                     LLRS"
     gene            12685..>13962
                     /locus_tag="VHE37_11510"
     CDS             12685..>13962
                     /locus_tag="VHE37_11510"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HVT36207.1"
                     /translation="MKQVLIGAACALLLAGCQQSAVPEVGGGSAPLKDAFPVARATVT
                     GPIGDAGIHGHALWDSWFDLSELGYDEQEYFISGTAQVQPGGATAPYTTRIIVARPKD
                     ASRFNGTVVMDWVNVTAQFENAVDTTEAHDMMLREGYAYVLVSAQAAGLCCVPELTPK
                     TWDPVRYMAISHPGDDWSFDMFAQVARALRDADPAGVDPMAGLPVQKVLAWGQSQSAD
                     RLYDYVNTGYADSRVIDGFIIHGGGSKFFDNPLPVPVIHLLSDREALNDTPTADPNYS
                     LWEIAATAHTDIYVGWHQVGGESTRALADAPRQPASADPQLHVTAGNYGEQVSPLLAA
                     CIVAGSAMPMRYAVKASLHYLDAWVRGGPRPPQGARFEFDSSGKLAKDEHGNTRGGIR
                     YPPVDVPVARYDSTACPLGGITLPFTDAQLAQLY"
BASE COUNT         2412 a         4596 c         4589 g         2365 t
ORIGIN      
        1 cagcgtgccg atgcctacga tggcaatgtc ggaaggcttc ggcgcgccga tctgcggttc
       61 gagcagctgg ctcaggtgct cggcagcgcc gcggcagacg tgatcatgca gctgttcgat
      121 ggtctgcgtc gcatgccgca acgtggcgac ctggccgatc atgtacatgc cggccgcgag
      181 ttgctcgggc ggcgcgacgt cgacgatctt gccgtcggca tcgcgattga cgcccttgga
      241 agcgatgcgc agacggccca ggttgaggtc ctccagcgtg tcgcggattt cctcggcgct
      301 tttcccttcg ccgatcaggc ggcggcgagt ttcatagaac tcgtgcgcaa agcgcgtgtc
      361 gacgcagcgg ctggcgtggc ccggcccggt ttccagattc atggtgcgtt cgcagccgag
      421 cgcctgtttc tggaagccgg caaccaccgc gccactgctg acgatttcct cggtgaacag
      481 gtaagccgta cccatcaacg cgccaacctt gatgccgcgc gccgtcagcg gagccgtcat
      541 cgcagccacc atcgccgccg acaatgcatc gtggatgccg ccggcgaaaa gcacatgcac
      601 ctggctttcc tcgccgggct tcacctccgc gagcagcact tcgatcatct gctcccacag
      661 tgcaaaactg gcgagcggac ccacgtgccc gccgcactcg cggccttcga acacgaatcg
      721 ccgcgcgccc tgctcgagat acaggcgcag cagcgcgggc gcaggagcat ggatgtaggt
      781 ggtgatgccg cgcttttcga attccgctgc ctggtctggc cggccgccgg cgatcagtgc
      841 gaaatccggc ttgcatttcc agatctccgc gcattgttct tcacgcagtg catgcggtac
      901 gaaaccgagc atgcccacgc cccacggcag ggaaccgatg cgggccttgg tgtccgccag
      961 catcctggca acttgcggtc cgcgcatcag ggccagcgcc agcaagggca gggcgccgcc
     1021 gcgggcgacg gcagcagcaa aatcggcgct gtcggagacg cgcgtcatcg gcccctgcac
     1081 cagtggatag cgggtgccgt gcgaagccgc gagcggagcg ttttcgccca gcgcttgctg
     1141 ctgcgcagcc agttccacct gcttcatggc cgcgcggcgc acggcgcgga tcagcttcgc
     1201 tgcgctgccg aactgtgtcc ggtaagccgc cgccaagccg atcccctggc caatcggcag
     1261 cagctgcgaa ccatcgccat tccagcccat gcgtgatgcg agccgcgctt tccattcggc
     1321 gagcggcagc tggccgccct ccgcctgccg cagatcttct tcggctgcct tgagcacggc
     1381 cgacccggca cgggcgtagg cgcggcacgg agcttcgagc agttcgccga acagccggca
     1441 ctcagcgccg ttcaggcggg cgagttccga cttgagtggt tcgggcagcg gcgactcatc
     1501 cagcagcagc agctgttcgt cgaggaccac gcctgccgca ccggcgacgc ggcaggcggc
     1561 agccgaatgc acgcctacac tgccgcgcgc ataaaccggc agggacgtct tgccggccag
     1621 cttctgcagc aggatgaaac tggtgtcttc gccgacccag ccgccgcctt cgtgtcctag
     1681 cgcaaccagc ccttgaaacg aagcgggaac atttgcgagc tgactggcgg ccgtgatctc
     1741 cgccagcatc cgcacctgcg cagtcggtgc aagctgctct gcgaccgatg caaagtcgcc
     1801 acacgcgatg acgaccatcg gccgcgctcc cgcggcggcc agcaggtcgc gcgccagtgc
     1861 cgaacgtgcc ggcgtgaccc gcaggccgag ggcgccgtgc tgcgaggaga gcaacccggc
     1921 gaagttgcgc attgccgcgg cctcatcgcg cacctgctcg agatcgagga tggcggtggc
     1981 gccgccgcgc agcagggcgc gcgccagtgt cggctcgaga tcggccgcca ccagcccaag
     2041 gacatcaaaa ttcttgctca tgtctcgcgc tccagcgcct gcgcagcctt aaacctttac
     2101 gccttccaga aacggcactt ccagaacctt ttcatgcacg atcggcccgt gaccgaaata
     2161 gccgttctcg ccaaaacatt cgccgtggtc cgcggtgatg atgaagtggg tgcccacggg
     2221 ggccttgctg atcagggcat cgagcacgcc atcgacatat tccacggttt tgatctgctg
     2281 acgatgtaga tctttcatct gggtgtcgtc gaagaacttg tcggccgtcg attggcccag
     2341 ctcgtcgtcc atgcgcttga acacgccgtg cacgccagaa atatgcggaa gcgacttggg
     2401 atcgagcatg tacgggtaat gcgtttcgcc gatgttgagg aaatggaagc tcggttcgga
     2461 atcatcgaac tcgatctcct tcaccatgcc ggcgaagtcg ttgtgactcg ccatcagctt
     2521 gtaatcgtcg aaatgcttgt tcaggtgcga aaagggattg agtaccggca gcgaaacctt
     2581 ggccacggtg cggtagccgt tttcctggag cacgtgcggc agcgaaagct gggggacgaa
     2641 agacttgaac gacaggtcgg gtacgcccag ccggtccacc cagcgcgtga actccttctt
     2701 gtagacctcc gaggcgaata caccttgggg cgactcgtgc ggaatcatcc ccatcagata
     2761 ggtgtaatgg gaaggagagg tccaggacgc atacgaatag cgcttgagac ccatgccgag
     2821 gcgatccatg ttgggacgcc gtgcagcggc atagctgtcg tagcggcagc tatccagaac
     2881 gatgaaaacg aggtttttgc tcatctgaaa ttacgaatgg cagcatccat actgcgcggg
     2941 gacgtggaac atcggcattc cattgccgca tgaagctcgc tttgagccac gtccattgtt
     3001 gcgcagccgc atggccgacc ggcaccctcc ctattcactt gtttttgtag gaaacccacc
     3061 cctttgcaag cactatagtc ttgatcggtg acgtggaaat gagttagatt caaaataaga
     3121 atctaagttt aagacatccg tcacactatg ggcacgcgcg agcgccggca acgggaagtt
     3181 gccgagcgcg aaaaacgttt tgtcgaccgc gcgcgtgaac tgatccgcgt ggaaggtttg
     3241 ctgaacctgc agatgtcgcg ggtggctgat ggttgcgact acgcggtcgg cacgctgtac
     3301 cagcacttct cctcgaagga ggacctgctg ctggcggtca cgaccgagac ggcgcgcgag
     3361 cacgtggacc tgttccggcg cgtagccagc tggaacgcaa acacgcgcga ccgcatgttt
     3421 gccatcggcg tggccgacat gatcttcgtg cagcgcaatc ccgattattt ttccaccgtg
     3481 cagtacgcac tgtgcgaagt cgtctggggc gcggcgtcgc aggagcggcg cgatgaattt
     3541 ctcgaggtca accagccgat aacgcagctg gtcattggca tcgtcgacga tgcggtggca
     3601 aagggcgacc tgcagttggt cggctttacc ccgcagcagc tgacggtggg cctgtgggcg
     3661 ttgtgcctgg gcatgcacaa cctggtgcat gcagaaggcg tgctgcgtga tttcgacatc
     3721 aaccagccgt accgggccat gtgccggcac atgcagtaca tgctcaacgg cttcggctgg
     3781 aagccgctgt ccgatcctgc ggacgaagca gcgacagagc gacttataca aaggatcaag
     3841 tccgaggttt tccatgacct ggcgtgaatg ggtggcgctg gcgatgctgg cccttgcggc
     3901 ggctcccgcg gcgcaggcga tgactttgtc cgaggcctac gcgctggcgc tgcagcacga
     3961 cccggcagtg gccgaatctc tgggccagta caaggcggag cgcgagccgg gcgaccagga
     4021 gcgctcgacg ctttatccca acgtgtcggt cgcggcgggc gcagcctacg atcgcaccga
     4081 ttcgacgggc gtgttcggcc acagcaacga cacctatccg agctatagcg cgacgctcaa
     4141 gctgcgacag ccgctgttcc ggctggactg gtttgcgcgc ggcgcgcgtg cagatgcccg
     4201 ggacgccagc gccgatgcat cactggcgca gcgcaagctc gacctgctgc agcaggtggc
     4261 gcaacgctac ttcgatgtgc tgaccgcgca ggatggcctg gcgcagaccc aggccgaggc
     4321 ccgggcggtg cgcgagtcgt tcgatgccac gcgcaagcgc tatgaggtgg aactggcgcc
     4381 gggcaccgac ctgaaggaag cgcaggcccg caacgacctg gcgggcgcct ccatgctggc
     4441 ggcgcgccgt acgctggaag ccgcgctcga cgcattggac gaaagcaccg gcaacggccg
     4501 cgctgcactg ccgcggctgc cggcggagct gcgctttcca ccgctgctgc cggccgatgc
     4561 cgaaagctgg atcgccagcg ggcgcgagca cagccccaaa ttgctgctcg cgcaacagca
     4621 gctggcaatc gcccgcgccg atctcaccag ccgcaagtcc gaggccacgc cgacggtgga
     4681 cctggtcggc agctacggcc gtgatgacac cagccgttac gtgttcggac aaaaggttga
     4741 cgatggccgc gtcgggctcg aactgaacat cccgctctac gcgggcggct acaacgccag
     4801 ccgcgtgcgc gaggcgcagg gacggctgga gcaggcgcag tccgcacttg cgcgcaccca
     4861 ggccgatctg gagcgcgaca tccgcgcacg gctgcgcgac gtgcagctgt ccatggcgca
     4921 gatcgacgcc tatgaaaagc agctgggttc ggcgcaggtg gcgcaacagg ccacggccaa
     4981 cggctacgaa gccggtaccc gcaccatcag cgacgtgctc gatgccaaga gccgcgtggt
     5041 gcaggcgcag cgcgacctca accagagccg ctacagcgtg ctgctcaatc tgctgctgct
     5101 caagcaggcg gtcggcgtgc tgagcgagaa agacttcgcg gaaatcgacc ggctgctggc
     5161 gcaaggcacg gcagcctcaa cggaacagag tccatgacca agagaatgat catcatgatg
     5221 gccatcgccg ccgtcgtgtt cggcggcgtg tttttcatga gccacaacat gagcgtgggc
     5281 atggccgact acctggagca catgccgtcg ccggcggcgg ccatctccac caccaaggtc
     5341 agcaccatga cctgggacaa cacggtggat gccgcggcca cgctggtggc ggcccagagc
     5401 accaccgtta ccactgaagc gggcggcatc gtgcaggcca tccacttcga atcgggcgcg
     5461 catgtgcagc agggcgcgct gctggtgacg ctggacagcg ccaacgagca ggccgagctc
     5521 aagcgcctgc aggcgcaggc cgaactggcg cggctcaacc acgcgcgccg cgaaaagctg
     5581 tacggactcg aagctatctc gaaatccgat ctcgattccg ccgaaagcga ggccgccgcc
     5641 gcgcaggctg cggtggatgc gcagcaggcc aagctggcgc aaaagcagat ccgcgcaccg
     5701 ttcgccggcc agctcggcat ccgccaggtg aacgtcggcc agttcctcga tgccggcaag
     5761 cccatcgtca cgctgcaggc gctcgacccg gtgtacgccg acttcaacct gccggaacag
     5821 cagctcggcg tggtcaccgc gggcctcgcg gtggaagccg aggtggcggc atggcccggc
     5881 aagaagttcg gcggcaaggt ggtggcggtg gagccgcggg tggacgaggc gacccgcaac
     5941 ttccatgtgc gcgcgcagat ggacaacggc gaaggcaagc ttgccggcgg ccagtccgcc
     6001 cacatcacgc tgaagctgcc gggcgcgcgc acgctggtgg tggtgccgca caccgcgatc
     6061 agctacagct cctatggcag ctcggtgttt gtggtcggca agatggctca cccggcggac
     6121 aaggacgcgc cgcgcatgcc gcaggccggt ccgtggaccg atctcgaggt gacgcagcgc
     6181 ttcgtcaaga ccggcgagga gcgcggcgat ttcgtcgccg tgaccgacgg cctcaaggtg
     6241 ggcgaggaga tcgccacctc gggcctgctc aagctgcgca accagcagcc ggtgctgatc
     6301 aacaacgatg cgccgccgaa gccggagttg aatccgtctg tgccgcaagg ctgagcaagg
     6361 acgggactcg agatttacat gcacttcacc gacatcttca tcaagcgccc ggtcctggcc
     6421 acggtcatca gcctggtgat cttcctgctc gggctgcgcg cgtttcacga cctgcgcgtg
     6481 cgcgagtatc cggagctgca gaacgcgcag gtgacggtat cggtggcgta tcccggcgcc
     6541 gacccggcgc tgatggaagg cttcatcacc acgccgctgg agcgcgagat cgccaccgcc
     6601 gacggcatcg atttcctgtc gtcgtccacc ggccagggcg ccgccaccat caccgccaac
     6661 ctgcggctgg acaagaaccc caacctcgcg ctgacggaaa tttccgccaa ggtgaacaag
     6721 gtgcgcggcc agctgccgca ggatgccgag gacccggtga tcgaggtgtc cgagggcggc
     6781 ggtactgcgg ccatgtacct gaccttccat tccaaggtgc tggacaacag ccagatcacc
     6841 gattacctca cgcgcgtggt gcagccgcag ctgtcgtcga ttcccggcgt ggagaaggcg
     6901 cagatcatgg gcgagcgcac ctatgcgctg cgcgtgtggc tcaagcccga ggcgctggcc
     6961 gcgcacaaca tgaccgccag cgacgtctac cagcgcctgc gcgcgcagaa cgtgctgtcc
     7021 gcggtgggcg agatcaaggg caactacgtg cgcatcggcc ttgccgcgca gaccgacctg
     7081 cgctcgccgg atgaattccg ccagctggtg gtgaagaccg agggcagctc actggtgcgg
     7141 ctggccgaca tcgccgacgt cgcgctcggc gcggaaacct acgatggcgc cgccggctgg
     7201 gacggcgatc ccgcgctgtt tgtcggcatc gacatccgcc ccgacgccaa cctgctcgag
     7261 gtgatcgagc gcgtgcgcca ggtctggccc gacatcctgg cgcagctgcc gcaggggctg
     7321 gaaggcacca tcggctacga cagcaccgtc tacgtgcgcg acgcgatcaa ggaagtgaag
     7381 tccacgctga tcgaggcagt ggcgatcgtc atcatcgtca tcttcctgtt cctcggctcg
     7441 atgcggaact cgctgatccc ggcggtgacg gtgccgctgt cgctggtcgg cgcgctgttc
     7501 ctgatgctgg ccatgggctt caccatcaac ctgctgacgc tgctggccat ggtgctggcc
     7561 atcggcatgg tggtggacga tgccatcatc gtcatggaga acatccaccg ccacatcgag
     7621 cagggcatga cgccgctgca ggcttcgttc cagggcgcgc gcgaactggt cgggccgatc
     7681 atcgccatga ccatcacgct ggtggcggtg tacgcgccga tcggcttcct caccggcatc
     7741 accggaaaat tattcaccga gttcgccttc accctggccg gcgcggtgct gatctctggc
     7801 gtgctggcgc tgacgctgac gccgatgatg tgcgcgcgca tcctggcgcc gcaccacgca
     7861 gcgggcgggc agggcgggct ggccgactgg ctcgacgcca ggttcgaggg ctggcgcaac
     7921 ggctacaagc agcgcctgca cagcgcgctg gacacggtgc acgtgatcgg cgcgttcggc
     7981 gtgatcgtgc tggtgtcgtg cttcttcctg ttcaagcagc tgcccaacga actggcgccg
     8041 gaggaagacc gcggcttcgt cttcgccatc aacgaaaccg acggctattc ctcgcaggaa
     8101 tacctggacg ccaatttcca cctggcgcag aaagcggtgc tgcaggacaa ggacgtgccg
     8161 catatcttca ccttcagcct gggcgactcc ggcggcacca gccaggcctt catgggcatg
     8221 attctgcggc cgtggagcga gcgcaggcgc tcttcgacgc agatcatcaa cgagctgcaa
     8281 cccaggctgg cggagatttc cgcgctgcgt gcgcagatca tccagccgcc gccgctgccg
     8341 acgccagggc agggttaccc gctggagttc gtgctcaagg ccactgcttc gccggaagag
     8401 atcgcgcgcg tgtccgacca gatcgtggcg cgcgcgcagg ccagccacaa gttcttctac
     8461 atcgccacgc ggctgcgcat cgaccgtccg cagacggtga tcgaggtcga ccgcgacaag
     8521 gccgcgctgc tgggcgtgga catgtcggcg ctgtcggcgg acctgtccgc gctgctggcc
     8581 ggcggcgagg tcaaccgctt ctcctaccag agccgttcct accgcgtgat ccagcaggtg
     8641 gcgcgcgccg accgtctcaa tgccagccag ctcgaaggct tctacacgcg ggccgccagc
     8701 ggccagctgg tgccgatctc cacgctggtc acgctccggc agcgcaccat cgcgcgctcg
     8761 atcgagcacg cgcagcagct caatgccgac acgctggtgg cggtgcctgc cggcggcgtc
     8821 acccagggcg aagggctcaa ggtgttcgag gacctggcga aggaactcgc gccgcgcggc
     8881 taccaggtcg actactccgg cccgtcgcgc cagttcaagc aggaaggcag cgcgctgatg
     8941 gccaccttcg ccttcgcgct gatcgtcatc tacctggtgc tgtcggcgca gttcgagagt
     9001 ttccgcgatc cgctgatcat gctggtcacc gtgcccatgt cgatctgcgg cgcgctgctg
     9061 ttcctcaaga tcttcagcat gaccaacgac atgatgctca cgcatatctc cggcatgacg
     9121 ctgaacatct acacgcaggt cggcctggtc acgctgatcg gcgtgatcag caagcacggc
     9181 atcctgatcg tggaattcgc caacaagctg caggaacagg gcgtggccaa acgcgaggcg
     9241 atcgagcagg ccgcatcgat ccgcctgcgc ccggtgctga tgaccaccgc ggcgctggtg
     9301 gtgtcgatga tcccgctgct ggcggccagc ggccccggcg cggcggcgcg tttctccatg
     9361 gggctggtca tcgccagcgg catgggcatc ggcacgctgt tcacactcta cgtggtgccg
     9421 gcgatgtacc tctacatcgg gcgtgaccac gcgcaggcgc gagcaaaata gcggagccgg
     9481 tgtgcccgcc atggctgagt tccacgaaaa aaaccttaac cgcacctgcc cctggcatgt
     9541 tataaaaaat atattaatta aataattata agatagtttg taacagtgaa attgccggtg
     9601 cggtgctgct gtttttcgtc gccgtcaacc gcgcgctatg ctgcggcagg ccgggaatgg
     9661 gagcggggcg caacatgcca cgcgcggcct cgggggagat ggcatgaaga tgaaatggct
     9721 gtgggcgtgc gtgctgctgg ttttcgccct gcatgcgcag gccagggtgg ggcaggtgca
     9781 ccgctgcacc gatgccaagg gcgcgatggt gctcaccgac cagggttgcg aacaggatgc
     9841 gcagatcacc gcgcatcggc ccttcgtgca gccgcaggaa gtcgagccca cgccgctgcc
     9901 gtcctcgctg cagacagcgg cgccgccgac catccgcaac gcgccgcagc cggccgcagt
     9961 ccctgccgcg catagccccg acggtgccga ccgcgctgcc gccgccgcgg tgcgccgcgc
    10021 ctcgggcaag agcgccgcgg gcaacagcga tgaagattcg tctgccgcgc ctgcagatta
    10081 ttcgcagccc gcggacgacg agccggagat gtcgcagggc accaggctga tgctctggtc
    10141 cgggctgctg ctgggcttcg gtggttactt ctcgctggtg gtggtcgcct tccagcaatc
    10201 gaaaacctgg ggcgtgctgg tgctgctgtt ctcgccgctg gcggcggtga ttttcaagat
    10261 cacgcacaag ggtaaggggc gcatcccgtc gatcatcggt tccatcggca tggcgctggc
    10321 ggcgcgcgcg gtggcgctgc cgtccaccga cctgcacccc atggcggatc agcgcccaca
    10381 ggtcagcgcc acggcgccgg cggcgagcgg gcagcacggg cgcgagcagg aagccgattg
    10441 cgcgcgcacg gcgtcgatgg ctggcgttgg ctgcgccacc ggattggaat aatcgcctgc
    10501 tacgaaatca gtagcggaac cagtcggggc ggccggcggt ccacagctcg ccccacagct
    10561 tgccgccgcc gtcgacggtg agcacctcgc cggtgatgaa gctgccggag gggccgttga
    10621 ggtagatcac gctctcggcg atctccatcg gcgtgcccgg gcgcttcatc gggttggcgc
    10681 gcgggaactc ggcctgcgct tccggcggat agacctcgag cccttcggtc gccgtaagcc
    10741 ccggcgccac gcagttcacc cgcacctgca gcggcgccca ctcgaccgcg accgtgcgcg
    10801 tggcgccgat gatgccggcg cgcgccgcca ccgtgtgcgc cacgccgggc atgccgcgct
    10861 cggtgacgac cacgatgttg cagatcgcgc cgggcgtttt ctgctcgcgc caatactgcg
    10921 cggcgcgctg catcatgtac caggtgccgt tgaggttgtt gttgatgacc gcggcccagc
    10981 ccttgggtgc gtagtcgatc gaatgctgcg ggaactgccc gccagcgttg ttgaccaggt
    11041 gatccagccg gccgtgctcg gtgtggatct tcctgaacag ctcgtcgacc tgctcggcct
    11101 cgcgcacgtt gcaggcgtac gcgctcatgc gtgcgccgtg tccgtgcgcg aaggcggcga
    11161 ccgtgtcgag cttctcctgc gtgcgcccgc agatcaccac gcgcgcgccc aggcgcgcgt
    11221 acagcagcgc gaccgcgcgg ccgatgccgc tgccgccgcc ggaaaccagc gcaacctggt
    11281 ccttgaacaa atcgctgcgg tagaccgtcg gtgcggtcca cagctgctgc atcagctgcg
    11341 tcgattcggc gctcacgagc gcagcaactc gccggcgatg atgatcttgc gcacctcggt
    11401 ggtgccggcg ccgatctcca gcagcttgat cgagcggtac aggcggttga tctcggattc
    11461 ccagatgtag cccgagccgc cgtgcacctg caccgcctcg ttgagcacct ggttcatggt
    11521 ctcggccacg tacatcacac cggcggcggt gagcttgtgg atgtcgccgc ggccgccttc
    11581 gtcttcgctg atggtcgccg ccgcgcgcag cacctggtag gtgaacagtc gcatcgactc
    11641 gacccacacg tacatctgcg cgagcttgtc ctggatcatc tggaacgacg agatcgcctg
    11701 gccgaattgc ttgcgctggc gcgcgtagtc cagtgacagc gtcagcgcgc gttcggctat
    11761 tcctaagcag atcggcgaga tcatggcgcg ttcgagatcg agcccgctca tcaccacttt
    11821 cacgccgcgg ttttcttcgc cgacgagatt ttctgccggc accctgcact cgtcgaacac
    11881 cagttccgcc gtctgcgaac cgcggaaacc catcttgatc agcttctgcg ccaccttgaa
    11941 cccggggaaa tccttctcga cgatgaacgc gctgatgccc tgcgcgccct tgtccttgtc
    12001 ggtcttggcg tagaccagca gcacgtcggc cacaggtcca ttggtgatgt agatcttgcg
    12061 gccgttgagg atgtaatgcc cgccttcgcg ccgcgcggtg gtgcgcatgc tgcccagcgc
    12121 atcggagccg gcaccgggtt cggtcaggcc cagcgcgccg atcaacttgc ccttgcacag
    12181 gccaggcaaa tatttctttt tctgttcctc gttggcgttg cgcaggatgt tgtgcaggca
    12241 caggttctcg tgcgccaccc aggccagcgc cagcgcatgg ttccagcggc cgaaggcctg
    12301 caggatcagc cccgaggtca tcaggtccat gccggcgccg ccgtactgtt ccggcgcggt
    12361 cacgccgaag tagccagtct cgccgatctt gggaaagatg tccgtgggcc accattcctc
    12421 gtcgtccatg cgctgggcca gcggatagat ctccttctgc gcgaagcggt cggcctgcgc
    12481 caggatgtcc atctcgtcgg aagaaaggcc ggagaaggcc tgcatcacgg aaagtttgct
    12541 catgggtctt gcggattggt gagtcggact taccacgcaa gcttacggcg cggcctcgcg
    12601 gcgcggcttc acccgattgt aggagcggcc ggccgcgcca tctctgttct actgtgcggg
    12661 acgagagtac aggggagttg cgccatgaag caggtgctga tcggtgccgc gtgcgcgctg
    12721 ctgctggcgg gctgccagca gtcggccgtg ccggaagtcg gcggcggcag cgcgccgctg
    12781 aaggacgctt tcccggtggc gcgagccacg gtcactgggc caatcggcga cgccggtatc
    12841 cacggccatg cgctgtggga cagctggttc gacctgtccg agctcggtta cgacgagcag
    12901 gaatacttca tctccggcac cgcgcaggtg cagcccggtg gcgcgacggc gccctacacc
    12961 acgcgcatca tcgtcgcgcg gccgaaggac gcgtcacgct tcaacggcac cgtggtgatg
    13021 gactgggtca atgtcaccgc gcagttcgag aatgcggtcg acaccaccga ggcgcacgac
    13081 atgatgctgc gcgaaggcta tgcctacgtg ctggtgtccg cgcaggccgc gggattgtgc
    13141 tgcgtgccgg agttgacgcc caagacctgg gacccggtgc gctacatggc gatcagccat
    13201 cccggcgacg actggagctt tgacatgttc gcgcaggtgg cgcgcgcgct gcgcgatgcc
    13261 gatccggccg gtgtcgatcc gatggccgga ctgccggtgc agaaggtgct ggcctggggc
    13321 cagtcgcagt cggccgaccg cctctacgac tacgtcaaca ccggctacgc cgacagccgc
    13381 gtgatcgacg gcttcatcat ccatggcggc ggcagcaagt tcttcgacaa tccgctgccg
    13441 gtgccggtga tccacctgct gtcggaccgc gaggcgttga acgacacgcc gacggccgat
    13501 ccgaattact cgctgtggga aatcgccgcc acggcgcata ccgacatcta cgtgggctgg
    13561 caccaggtgg gtggcgagtc gacacgcgcg ctggccgatg cgccgcgcca gccggcgtcg
    13621 gccgatccgc agctgcacgt gaccgccggc aattacggcg agcaggtcag cccgctgctg
    13681 gccgcctgca tcgtcgccgg cagcgccatg ccgatgcgct acgcggtgaa ggcgtcgctg
    13741 cattatctag atgcctgggt gcgcggcgga ccgcgcccgc cccagggtgc gcgcttcgaa
    13801 ttcgatagca gcggcaagct ggccaaggac gagcacggca acacgcgggg cggcatccgc
    13861 tatccgcccg tggacgtgcc ggtggcgcgc tacgactcca cggcctgccc actgggtggc
    13921 atcacccttc cgttcaccga tgcgcaactc gcgcagctgt ac
//
LOCUS       DASZZC010000540         5853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1138382,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000540 DASZZC010000000
VERSION     DASZZC010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138382"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="VHE37_11515"
     CDS             complement(<1..1600)
                     /locus_tag="VHE37_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36208.1"
                     /translation="MLKKIAVFQLGLACGLTSGLVSAGVLGDVATGLLTRPAVRSAPA
                     TSVGALSLLPRAPGSTALAVPAAGGAALPGLGGAVTPAAGPLGITVLQPGADGSGYAY
                     ILALGSGGPAQTLIGNIIAGRLVTPLLGLDGNGGLLDVGVLDGNGTAQAGTLLGAAVL
                     SGSDTGLGPIGLGLIDDDNVGNGSQLGIAALSGNNAGNGGQIGIGVLNRTFSGNGGRM
                     GVGLLNGDASGNAGNVGIGILSGHHSGYGGTLSIGLLNAADPLHICSASNCVNLASLR
                     DMSTMLPGVDQSGVAVGGANGTVTGSNPLLNLGVLAGNHAGNGGAIGVAALSGNDAAQ
                     ASLIGVGVLNGSGTGSGKVAGVGLLSGGASANGGVAGVGVLNEAASGNGGTAGVAVLS
                     GSGSGNAGTLAAGVLNGNDSANSKMLSVGALNGSNSGNSSGIAAGVLNNNTSGNGGII
                     GAGVLNGSSSGNGGTIGAGALNGGGSGNGGSLGVGAVNGNGGGSSGGGASSGGGAGSG
                     TGTAQVPSDSCTFGVKDRRVREACE"
     gene            1909..2994
                     /locus_tag="VHE37_11520"
     CDS             1909..2994
                     /locus_tag="VHE37_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT36209.1"
                     /translation="MARVTILAGRRLEVGLDPSLGVHKFHSHWGSRLGGVPIGVAIGT
                     GLLLDAWLSGIDTSFSVAFIVCMLPAFGIGMAEDLTAKAGVATRLVFTMISSALGWWL
                     LLGKLTRIDVPQVDLLLASVPLAAFVLTLVASAGVAHAVNIVDGYNGLSGFYSVVVFL
                     SLAYVGWQVNDVMIVHVSLLFAAAVLGFLYWNFPYGLIFLGDAGAYVVGFAVGELSIL
                     LVSRNPEVSAWCPMLLAVYPVTETLFAIYRKQWLRGISPGLPDGLHFHMLIYKRLMKL
                     RFADNASRQMLMRNSATSPYLWIITLVTAAPAVMLWNHREPLMAACAIFALAYLWLYW
                     RIIRFRTPRFLVLRGNLVRSSLADRRA"
     gene            3011..3937
                     /locus_tag="VHE37_11525"
     CDS             3011..3937
                     /locus_tag="VHE37_11525"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVT36210.1"
                     /translation="MKVLVTGGAGFIGSHLVERLLLGGVSVSVVDDFSSGREANLVGV
                     AGHHSLQVLHKPVSALTVAELEPPAAIVHLASIPSAAQSWQSPLAAHEANLSSTVHLL
                     DLAARWRCRTFVLASSAAVYGNAEVPVRETAACQPQSPYGLHKLASEEYLRLLAPRAG
                     MQAVNLRLFNVYGPRQRADSEYAGVISRFAHAALANQPMRICGDGQQTRDFVHVDDVI
                     ESIMQSLEVSPAAADLRLNIGSGRAVSIDQLARMLARGAASTAGAVHIDAREGDIRHS
                     CADISRAAQVLGYRPAVTLEQGTARLLDALRG"
     gene            complement(3945..5717)
                     /locus_tag="VHE37_11530"
     CDS             complement(3945..5717)
                     /locus_tag="VHE37_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HVT36211.1"
                     /translation="MTKSPPSREHKLEFSEVLDALSADGLIKAAQVRDLTARQGMKGA
                     DQRHPLVIVAGYDIDNQQAPGKKLTLDTLVQWLAARSGLPYHRIDPLSIEVTKVTGVI
                     PYAYAARAKILPIKVTATELTVATCEPWLREWERELGPMAKRKITRVVSSPLDIERYV
                     VEFYALSRSVKASELENKKQKSGVQNLEQLIELGTRGKMDANDSHIVAIVDWLLQYAF
                     EQRASDIHLEPRRDHANMRFRIDGILHEVYQIPATVTAAVTSRIKVLSRMDLAEKRKP
                     QDGRIKTRTPAGKEVELRVSALPTAFGEKLVMRIFDPEVLVKDFRQLGLNPADEATWR
                     RMIGHPHGIILVTGPTGSGKTTTLYSTLKHLATPDVNVCTIEDPIELVEPSLNQMQVN
                     PAIDLNFADGIRALLRQDPDIIMVGEIRDLETAEIAVQAALTGHLVLSTLHTNDTVGA
                     ITRLGELGVPAYLIRATLLGVVAQRLVRVLCPHCKRKAPVTPEQWREWNGSGGGAAPP
                     FVYEPVGCLECRNTGYLGRAGVYEMLPITEELRAMIRDDTDLAHLKSAALKQGLLPLR
                     LAGLQKVAAGDTAAAEVLGTTPPL"
BASE COUNT          884 a         1938 c         1954 g         1077 t
ORIGIN      
        1 gctcgcaggc ttcacgcacg cgccggtcct tgacgccgaa ggtgcagctg tcggacggaa
       61 cctgcgccgt gccggtgccg ctgcccgcgc cgccgccgga actggcgcca ccgccgctgc
      121 tgccgccacc attcccgttg actgccccca caccgaggct gccgccatta ccgctgccgc
      181 cgccatttag cgccccggcg ccgatggtcc cgccgttacc actgctgctg ccgttgagca
      241 cgccggcgcc gatgattccg ccattgccgc tggtgttgtt gttcagcacg ccggcggcga
      301 tgccgctgga gttgccgctg ttgctgccgt tgagcgctcc cacggagagc attttgctgt
      361 tggcggaatc gtttccgttg agcacacctg cggcgagcgt tccagcattt ccgctgccgc
      421 tgccggagag caccgcgacg cccgcagttc cgccattgcc ggaggcggct tcgttgagca
      481 cgccgacgcc ggcgactccg ccgttggcgc tggcgccgcc gctgagcaat ccgacgccgg
      541 ccaccttgcc gctgccggta ccgctgccgt tgagcacgcc gaccccgatc agcgaagcct
      601 gcgccgcatc gttgcccgag agcgcagcca cgccgatggc gccgccgttg ccggcgtggt
      661 tgccggccag cacgccgagg ttgagcagag gattgctgcc ggttactgtt ccattggcgc
      721 cgccgacggc tacgccggac tggtcgacgc ccgggagcat ggtcgacatg tcgcgcaggc
      781 tggccaggtt gacgcagttg ctggcgctgc agatatgcaa cggatcggcg gcattgagca
      841 gcccgatact cagcgtgccg ccgtacccgc tgtgatggcc ggacaggatg ccgattccca
      901 cgtttccggc gttgcccgaa gcatcgccat tgagcaggcc cacacccatg cggccgccgt
      961 tgccggagaa cgtgcggttg agcacaccga tgccaatctg gccgccattg ccggcgttgt
     1021 tgccggacag cgcggcgata cccagttgcg agccgttgcc gacgttgtcg tcgtcgatga
     1081 ggcccaggcc gatcggcccc agcccggtgt cggagcccga cagcacggca gcgcccagca
     1141 gcgtgccggc ctgcgcggtg ccgttgccgt cgagcacgcc cacatctagc aggccgccgt
     1201 tgccgtcgag cccgagcagg ggggtgacca gccgcccggc gatgatgttg ccgatgaggg
     1261 tctgcgccgg gccaccggac ccaagcgcaa ggatgtaggc atagccggag ccgtctgcgc
     1321 cgggctgcag cacggtgatg cccaacgggc ctgcggctgg cgtgaccgct ccgccgagac
     1381 cggggagagc agcgccgccg gctgccggga ctgcgagcgc ggtggacccc ggggccctgg
     1441 gaagcaggga cagggcaccg acagaggtgg cgggcgccga ccgcacagcc ggccgcgtca
     1501 gcaggcccgt tgcgacgtca cccagcaccc cggcagagac cagcccggaa gtcagcccgc
     1561 aggccaggcc cagttggaac acggcgattt tcttcaacat gaaacggcac tccctcaaat
     1621 aaccggttac acgatatcgc aaagcaggcg tgcccagacc tgaaatggcc gcaatcttgc
     1681 tagagaagaa cttgtcagca aaagtcgcaa cggccgtaaa gaaatcgcgc gggggcgggc
     1741 gaatgtggaa gttttcacgc tgtgcctgtg acttgaatca cccggaacat tgcggaaatc
     1801 gcacctggcc cgatatcctt cgcgcccagt caggggagtc tcggttgcgc aatgcaaact
     1861 gaagtgttac tgccagtttg gctgttcacc acgctaacca gcatctggat ggcacgcgtc
     1921 accatactcg ccggccgccg cctcgaggtg ggactggacc caagcctggg tgtgcacaag
     1981 ttccacagcc actggggttc ccggctgggt ggcgtgccca ttggcgtggc catcggcacc
     2041 gggctgctgc tcgacgcctg gctgtccggc atcgatacca gtttcagcgt ggccttcatc
     2101 gtctgcatgc tgccggcgtt cggtatcggc atggcagaag atctcaccgc caaggcgggt
     2161 gtggccacgc gcctggtctt caccatgatc tcatcggcgc tcggctggtg gctgctgctg
     2221 ggcaagctga cgcgcatcga tgttccgcag gtcgatctcc tgctggcttc ggtcccgctg
     2281 gccgcgttcg tgctcaccct ggtcgcctcg gcgggcgtgg cccatgccgt caacatcgtc
     2341 gacggctaca acggcctgag cggtttctat tcggtggtgg tgttcctgtc gctggcctat
     2401 gtaggctggc aggtcaacga cgtgatgatc gtgcatgtgt cgctgctgtt cgccgcggcc
     2461 gtgctcgggt tcctctactg gaatttccct tacgggctga tcttcctcgg cgatgctggt
     2521 gcttatgtcg tcggcttcgc ggtgggcgaa ttgtcgatcc tgctggtgag ccgcaacccg
     2581 gaagtatcgg cctggtgccc gatgctgctg gcggtctacc cggtgacgga gaccctgttc
     2641 gccatctacc gcaagcaatg gctgcgcggc atctcgccgg gtctgcccga tgggctgcat
     2701 ttccacatgc tgatctacaa gcgcctgatg aagctgcgct tcgccgacaa tgcctcgcgc
     2761 cagatgctga tgcgcaactc ggcgacgtcg ccatacctgt ggatcatcac cctggtcact
     2821 gccgcgccgg cggtgatgct gtggaaccac cgcgaaccct tgatggcagc ctgcgccatc
     2881 ttcgcccttg catacctgtg gctgtactgg cgcatcatcc gcttccgcac gcctcgcttc
     2941 ctggtgctgc gcggcaacct ggtgcgctcc agcctggctg atcgccgcgc ctgagtccgc
     3001 tgccggcgct gtgaaagtcc tggttaccgg cggcgccggg ttcatcggtt cccacctggt
     3061 cgagcgcctg ctgctcggcg gcgtcagcgt gtcggtggtc gacgatttct ccagcgggcg
     3121 cgaggcgaat ctcgtcggcg tcgccggtca tcactccctg caagtgctgc acaaaccggt
     3181 gagcgccttg accgttgcgg agcttgagcc gcccgcggcc atcgtgcacc tggcttcgat
     3241 tccgtctgca gcgcagtcat ggcaatcgcc actggcggcg cacgaggcca atctgagctc
     3301 caccgtgcac ctgctcgatc tcgccgcacg ctggcgctgc cgcaccttcg tgctggccag
     3361 ttcagcggcg gtgtatggca atgccgaagt gccggtgcgg gagacggcgg cgtgccagcc
     3421 gcagtcgcca tatggcttgc acaagctggc cagcgaggaa tatctgcgcc tgctggcgcc
     3481 gcgtgccggc atgcaggctg tgaacctgcg cctgttcaat gtgtacggtc cgcgccagcg
     3541 ggctgactcc gagtacgcgg gcgtgatctc gcgcttcgcg catgcggcgc tggcgaacca
     3601 gcccatgcgc atctgcggcg acggccagca gacgcgagat ttcgtccatg tcgacgacgt
     3661 gatcgaatcc atcatgcagt cgctggaagt gtcgcccgcg gcagcggacc tgcgcctcaa
     3721 catcggcagt ggacgcgcgg tgtcaatcga tcagttggcg aggatgctgg cgcgaggcgc
     3781 ggccagcacg gccggggcag tacatatcga tgcccgcgaa ggcgacatcc gccattcctg
     3841 cgccgatatc tcgcgtgccg cgcaggtgct gggttaccgg ccggcagtga cgctcgagca
     3901 gggaaccgcg cgcctgctgg atgcactgcg cgggtagcgt ttcctcaaag cggtggtgtg
     3961 gtgccgagca cttcggcagc ggcggtgtcg cctgcggcca ctttctgcag tccggccagc
     4021 cgcagcggca gcagcccctg cttgagcgcg gccgacttca gatgcgcaag gtcggtgtcg
     4081 tcgcggatca tcgcgcgcag ttcctcggta atcggcagca tttcgtagac gccggcgcgg
     4141 ccgaggtaac cggtgttgcg gcattccagg cagcccaccg gctcgtaaac gaagggcggc
     4201 gctgcgcctc cgccggagcc gttccattcg cgccactgtt cgggcgtcac cggcgccttg
     4261 cgcttgcagt gcgggcacag cacgcgcacc aggcgttgcg caaccacgcc cagcagcgtg
     4321 gcgcggatca ggtaggcggg tacgcccagt tcgcccaggc gggtgatggc gccgacggtg
     4381 tcgttggtat gcagcgtaga cagcaccagg tggccggtga gcgccgcctg caccgcgatc
     4441 tccgcggttt ccaggtcgcg gatttcgccg accatgatga tgtccgggtc ctggcgcagc
     4501 agcgcgcgga tgccgtcggc gaaattcagg tcgatggccg ggttcacctg catctggttc
     4561 agcgaaggct cgaccagttc gatcggatct tcgatggtgc agacgttgac gtccggcgtg
     4621 gccaggtgct tcagcgtgga atacagcgtg gtggtcttgc ccgagccggt gggtccggtg
     4681 accaggatga tgccgtgcgg atgcccaatc atgcgccgcc aggtggcttc gtccgccggg
     4741 ttgaggccga gctggcggaa atccttgacc agcacctcag gatcgaaaat acgcatgacc
     4801 agcttctcgc cgaaagccgt gggcagtgcg gacacgcgca gttccacctc tttgccggcc
     4861 ggcgtgcgcg tcttgatgcg gccgtcctgc ggcttgcgtt tctccgccag gtccatgcgc
     4921 gacagcacct tgatgcggct ggtcaccgcc gccgtcaccg tggcggggat ctggtagacc
     4981 tcgtgcagga tgccgtcgat gcggaagcgc atgttggcgt gatctcggcg cggctccaga
     5041 tggatgtcgc tggcgcgctg ttcgaaggcg tactgcaaca gccagtcgac gatggcgacg
     5101 atgtgcgagt cgttcgcatc catcttgccg cgcgtgccca gctcgatcag ctgctccaga
     5161 ttctgcacgc cgctcttctg cttcttgttc tccagttcgc tggccttgac cgagcgcgac
     5221 agcgcgtaga actccaccac gtaacgctcg atgtcgagcg ggctggagac cacgcgggtg
     5281 atcttgcgct tggccatggg gccgagctcg cgttcccatt cgcgcagcca aggctcgcaa
     5341 gttgccacgg tcaattcggt ggccgtgacc ttgatcggca ggatcttggc gcgcgcggcg
     5401 taggcatagg gaatgacgcc ggtgaccttg gtcacctcga tcgacagcgg atcgatgcgg
     5461 tgatagggca ggcccgagcg cgcagcgagc cactgcacca gcgtatcgag cgtgagcttc
     5521 ttgccgggtg cctgctggtt gtcgatgtca taaccggcga cgatcaccag ggggtgccgc
     5581 tggtccgcgc ctttcatgcc ctggcgcgcg gttagatcgc gcacctgcgc cgccttgatc
     5641 agcccgtcgg cggacagcgc gtcgagcacc tcggaaaatt ccagcttgtg ctcgcgcgag
     5701 ggcggggact tggtcatgcg gcctacgtta gcacgagcgg agccgcggcc aaacggcatt
     5761 agaatgcagg gcatgatccg cttccatggc gcctccgcac cgatgatgaa gaacctgttc
     5821 gcggcaatgc tgcccctgct gttgtgcgcc tgc
//
LOCUS       DASZZC010000541         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_298803,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000541 DASZZC010000000
VERSION     DASZZC010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298803"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VHE37_11535"
     CDS             complement(<1..1346)
                     /locus_tag="VHE37_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT36212.1"
                     /translation="MKSATDISEHLPLQRMYRWERERADQVYLTQPMGNGVVRDFTWR
                     ETMDQVRRMAAHLQSLGYAPGTHIALMSKNCAWWVMADFAIFMAGYVSVPLYPTLAAE
                     TVRQILTHSEAKLLFVGKLDDWPQMKAGVPAGLPCITFPVSPRDESLKDFPTWDDIVA
                     KTSPLPGNPLRDAGELATIIYTSGTTGMPKGVMHSFGNFAHAAVIGHSRVQARGDDRM
                     LSYLPMSHIAERISVELGSLRAGFRIYFAESLETFARDLARARPTLFFSVPRLWVKFQ
                     QGVQAKMPQEKLDRLLRIPLVGALVKKKILKGLGLDQCRFAGGGAAPMPPAMLNWFAR
                     LGLEILEVYGMTENCGLSHANESGKTKPGTVGTTYTGVQHRIDPATGEVQMKSAAVMQ
                     GYYKEPEKTRETFTDDGWLKTGDKGEIDSAGRLKITGRVKDLFKTSKGKYVAPAPI"
     gene            1424..>2275
                     /locus_tag="VHE37_11540"
     CDS             1424..>2275
                     /locus_tag="VHE37_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT36213.1"
                     /translation="MKSPYYTDDHEAFRAQVRRFVEKECAPHIEAWEQAEELPRELHR
                     KAGVLGLLGIGFPESIGGTETPDPFYTIVLTEELARAGSGGLIASLMSHGIGSPPIVH
                     TGTPAQKERWVRPVLAGEKIAALAITEAGGGSDVANLKTRALREGDQFIVNGSKSFIT
                     SGMRADYITTAVRTGGDGMGGVSLLVIEAGTPGFTRTPMHKMGWWCSDTANLYFDNCR
                     VPAENLVGRENQGFMAIMLNFNNERLMLAAQAWAFAQVCYEESLAYARERQTFGKPLI
                     RNQVIRHK"
BASE COUNT          373 a          770 c          744 g          388 t
ORIGIN      
        1 tcgatcggcg ccggcgccac gtacttgccc ttgctggtct tgaacaggtc tttcacccgg
       61 ccggtgatct tcagccgccc cgcgctgtcg atctcgccct tgtcgccggt cttgagccag
      121 ccgtcgtcgg tgaaggtctc gcgcgtcttc tccggctcct tgtagtagcc ctgcatcacc
      181 gcggcgctct tcatctgcac ctcgccggtg gccgggtcga tgcgatgctg cacgccggtg
      241 taggtggtgc ccaccgttcc gggcttggtc ttgcccgatt cattggcgtg cgacaggccg
      301 cagttctcgg tcatgccgta cacctcgagg atctccagcc ccaggcgcgc gaaccagttg
      361 agcatggccg gcggcatcgg cgccgcgccg ccgccggcga agcggcattg atccaggccc
      421 aggcccttga ggatcttttt cttcaccagg gcgccgacga gggggatgcg cagcagccgg
      481 tcgagctttt cctgcggcat cttcgcctgc acgccctgct ggaatttcac ccacagccgc
      541 ggcaccgaga agaacagcgt gggccgcgcg cgcgccaggt cgcgcgcgaa ggtttcgagc
      601 gactcggcga agtagatgcg gaagcccgcg cgcagcgagc cgagctccac gctgatgcgc
      661 tcggcgatgt gcgacatcgg caggtacgac agcatgcggt cgtcgccgcg cgcctgcacg
      721 cgcgagtggc cgatgaccgc cgcgtgcgcg aagttgccga agctgtgcat cacgcccttg
      781 ggcatgccgg tggtgccgga ggtgtagatg atcgtcgcca gctcgccggc atcgcgcagc
      841 gggttgccgg gcagcggcga ggtcttcgcc acgatgtcgt cccaggtcgg gaagtccttg
      901 agcgattcat cccgcggcga caccgggaag gtgatgcacg gcaggcccgc cggcacgccg
      961 gccttcatct gcggccagtc gtcgagcttg ccgacgaaca gcagcttcgc ttcgctgtgc
     1021 gtgaggatct gcctcaccgt ctccgccgcc agcgtgggat acagcggcac cgacacgtag
     1081 ccggccatga agatggcgaa gtcggccatc acccaccacg cgcagttctt cgacatgagc
     1141 gcgatgtgcg tgcctggcgc gtagcccagc gactgcaggt gcgcggccat gcgccgcacc
     1201 tggtccatgg tttcgcgcca ggtgaaatcg cgcaccacgc cgttgcccat cggctgcgtg
     1261 agataaacct ggtccgcgcg ctcgcgctcc cagcggtaca tgcgctgcag cggcaggtgc
     1321 tcggaaatat ccgttgcgct tttcatgtcc atcactctcc tctgccattg gtcttctgcc
     1381 aatgcttgcg ccatcctaac gcagccccca gaaaattttt ccgatgaaaa gtccctacta
     1441 caccgacgac cacgaagcct tccgcgcgca ggtgcggcgc ttcgtggaaa aagaatgcgc
     1501 gccgcacatc gaggcctggg aacaggcaga ggaactgccg cgcgaactgc accgcaaggc
     1561 cggcgtactg ggcctgctgg gcatcggctt tccggaaagc atcggcggta ccgagacgcc
     1621 cgacccgttc tacacgatcg tgcttaccga ggaacttgcg cgcgccgggt ccggcgggct
     1681 gatcgcctcg ctgatgagcc acggcatcgg ctcgccgccg atcgtgcaca ccggcacgcc
     1741 ggcgcagaag gagcgctggg tgcggccggt gctggcgggc gagaagatcg ccgcactggc
     1801 catcaccgag gccggcggcg gctcggacgt ggccaacttg aaaacccgcg cgctgcgcga
     1861 aggcgatcag ttcatcgtca acggcagcaa gagcttcatc acctcgggca tgcgcgccga
     1921 ctacatcacc accgcggtgc gcaccggcgg cgatggcatg ggcggcgtgt cgctgctggt
     1981 gatcgaggcc ggcacgccag gcttcacccg cacgccgatg cacaagatgg gctggtggtg
     2041 ctcggacacg gcgaacctgt acttcgacaa ctgccgcgtg ccggccgaaa acctggtcgg
     2101 ccgcgagaat cagggcttca tggccatcat gctcaacttc aacaatgaac ggttgatgct
     2161 ggccgcgcag gcctgggcct ttgcgcaggt gtgctacgag gaatcgctcg cctacgcgcg
     2221 ggagcggcag accttcggca agccgctgat caggaaccag gtcatccgcc acaag
//
LOCUS       DASZZC010000542         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1198149,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000542 DASZZC010000000
VERSION     DASZZC010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198149"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VHE37_11545"
     CDS             complement(<1..1333)
                     /locus_tag="VHE37_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36214.1"
                     /translation="MTPALPAAPESGPNLTVPILAATAVLSVALLALYGGPLSFGNLM
                     LALLMVNLAMLPAAFYLGPGRRVDFPIFELLLSYYAVSFGWTAFADKLEWRDLTQPQI
                     TRAFLVAFDGVLALMVGYYLVRLQMRLPIRGPAFARPLPAHLTFLMGVLMLAANTGLF
                     LIGGRELGGAMEQVRVTVGGFGLALCWLHYFKAEAQPAERLLIVGLTVLMMLIQALVA
                     SIKPLIVPLVVIAVSYWRSRHRVPWGMMALVVVVFAVINPVKHYWRDRVWHAGHDVTL
                     MENLGYLADAFEQYYFGDAVIEKDSKTAAINRVSDIAILGRAAYYTPKYIPYWRGVTL
                     KVLVYSMIPRSLMPDKPRSAFGNPFGRIYEIIGPNDYLTLISVNWVTEVYINFGPGGV
                     FLGMMLIGCLTRVIQESASSPGVPDLQFAVGLGAAGPLFNGGENLAMVWGGF"
     gene            complement(1341..2408)
                     /locus_tag="VHE37_11550"
     CDS             complement(1341..2408)
                     /locus_tag="VHE37_11550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVT36215.1"
                     /translation="MADAVVPSAVDPRPLKVLIHRTGSLGDTLVALPSLRLIAAAFPA
                     AQRVLLCNAPKSAVAAPAMAVLDGLGLVQRAVTYQSTGGRATALAVFRAVRAERPDVV
                     VSLSAKHGAGTALRDWLLFKLAGVPRVIGLGRSGRALRMIAPDRYETEAQRLARTISA
                     LGSIDWNNAAAWSIATTAPAAIPQQPYFCIAVGTKQPANDWGQDRWLELVQQLAASAP
                     ELRLVCVGSAEDHARSAELLARWPPGGDNLCGALSIAQTAAVLSHARLLICHDSGPMH
                     LAAAAGTRCVAVYSRRNRMGQWFPLGTAHVQVYAEVPCAGCGLTVCAVQGNRCTRLIS
                     AGEVAAQVQRAWSGPAAEPRV"
     gene            complement(2401..>2837)
                     /locus_tag="VHE37_11555"
     CDS             complement(2401..>2837)
                     /locus_tag="VHE37_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36216.1"
                     /translation="RRLMRRGRVRVAREINEQYETDGRSADLQEHLLHEPFIKGVSHW
                     VVRHNQYSTMEAQHRAADAAQPLALRDLRSADATVRRAAHKRIAYRLPGRPLWLLLYL
                     LFIRGAVLEGYAGIAYSGLRAWYELLIDIKTRELQRARALHG"
BASE COUNT          468 a          976 c         1003 g          390 t
ORIGIN      
        1 ggaagccgcc ccacaccatg gccaggtttt cgccaccgtt gaacagcggg ccggcggcac
       61 ccaggcccac ggcgaactgc aggtccggca cgccgggcga actggcggat tcctggatca
      121 cccgcgtcag gcagccgatc agcatcatgc cgaggaacac gccgccgggg ccgaagttga
      181 tgtacacctc ggtgacccag ttgaccgaga tcagcgtgag gtagtcgttg gggccgatga
      241 tttcgtagat gcggccgaac gggttgccga aggcagagcg cggcttgtcc ggcatcaggc
      301 tgcgcgggat catcgagtag accagcacct tgagcgtcac gccgcgccag taggggatgt
      361 acttgggcgt gtaataggcg gcgcggccga ggatggcgat gtcggacacg cggttgatcg
      421 ccgccgtctt gctgtccttc tcgatcacgg cgtcgccgaa gtagtactgc tcgaaggcgt
      481 cggccaggta gccgaggttc tccatcaggg tgacgtcgtg gccggcatgc cagacgcggt
      541 cgcgccagta gtgcttgacc gggttgatca ccgcgaacac caccaccacc agcgccatca
      601 tgccccaggg cacccggtgg cgcgagcgcc agtaggacac cgcgatcacc accagcggca
      661 cgatcagcgg cttgatcgag gccaccagcg cctggatcag catcatcagc acggtcagcc
      721 cgacgatgag caggcgctcg gcgggctgcg cctcggcctt gaagtaatgc agccagcaca
      781 gggcgaggcc gaagccgccc acggtgacgc gcacctgttc catggcgccg cccagttcgc
      841 ggccgccgat caggaacagg ccggtgttgg cggcgagcat cagcacgccc atcaggaagg
      901 tcaggtgcgc cggcagcggc cgcgcgaagg ccgggccgcg gatcggcagg cgcatctgca
      961 ggcgcaccag gtagtagccg accatcagcg ccagcacgcc atcgaacgcc accaggaagg
     1021 cgcgggtgat ctgcggctgc gtgaggtcgc gccattccag cttgtcggcg aacgcggtcc
     1081 agccgaagct gacggcgtag tacgacagca gcagttcgaa gatggggaag tcgacgcgtc
     1141 ggccggggcc caggtagaac gccgcaggca gcatggccag gttgaccatc agcagcgcca
     1201 gcatcaggtt gccgaacgac agcgggccgc cgtagagcgc cagcagcgcc accgacaaca
     1261 ccgcggtggc ggccaggatc ggaacggtca ggttgggccc gctttccggg gccgccggca
     1321 acgcgggggt catgcctcga ttatacccgc ggctccgcgg ccggccccga ccatgcgcgc
     1381 tggacctgcg cggccacctc accggcgctg atcaggcgcg tgcagcggtt gccctgcacc
     1441 gcacacacgg tgagcccgca gccggcgcac ggcacttcgg catacacctg cacgtgtgcc
     1501 gttcccagcg gaaaccattg ccccatgcgg ttgcgccgcg aatacaccgc cacgcagcga
     1561 gtgccggccg cggcggccag gtgcatcggt ccgctgtcgt ggcagatcag cagccgcgca
     1621 tgcgacagca ccgccgcggt ctgcgcgatg gacagcgcgc cgcacaggtt gtcgccgccg
     1681 ggcggccagc gcgccagcag ttccgccgag cgcgcgtggt cttcggccga gccgacgcag
     1741 accaggcgca gttccggcgc cgatgcggcc agctgctgga ccagttcgag ccagcggtcc
     1801 tggccccagt cgttggccgg ctgcttggtg cccacggcga tgcagaaata tggctgctgg
     1861 ggaatcgcgg ccggcgccgt ggtcgcgatc gaccaggctg ccgcattgtt ccagtcgatg
     1921 ctgcccagcg cggaaatggt tcgcgccagg cgctgtgctt cggtctcgta gcggtcgggc
     1981 gcgatcatgc gcagcgcgcg gccggaccgg ccgagcccga tcacgcgcgg cacgccggcc
     2041 agtttgaaca gcagccagtc gcgcagcgcg gtgccggcgc cgtgcttggc cgacagcgac
     2101 accacgacgt ccggccgctc cgcgcgcacc gcgcggaaca ccgccagtgc cgtcgcgcgg
     2161 ccgccggtac tctggtaggt caccgcacgt tgcaccaatc cgagtccgtc gagcaccgcc
     2221 atcgccggcg ccgcgacggc ggacttcggc gcgttgcaca acagcacgcg ctgtgccgcg
     2281 ggaaatgccg cggcgatcag ccgcagcgac ggcagcgcca ccagcgtgtc gccgagcgag
     2341 ccggtgcggt ggatcagcac cttgagcggc cgtggatcga ccgcggaggg aacgacggcg
     2401 tcagccatgc agcgcacgcg cgcgttgcag ctcgcgcgtc ttgatgtcga tgagcagttc
     2461 gtaccaggcg cgcagcccgc tgtaggcaat gccggcatag ccttcgagca cggcgccgcg
     2521 gatgaacagc aggtacagca gcagccacag cggacggccg ggcaggcgat aggcgatgcg
     2581 cttgtgcgcg gcgcggcgca cggtggcatc ggccgagcgc aggtcgcgca gcgccagcgg
     2641 ctgcgcggca tcggccgcgc ggtgctgcgc ttccatggtc gagtactggt tgtgccgcac
     2701 cacccagtgg ctcacgccct tgatgaacgg ctcgtgcagc aggtgctcct gcaggtcggc
     2761 gctgcggcca tcggtttcgt actgctcgtt gatctcgcgc gccacgcgca cgcgtccgcg
     2821 gcgcatgagt cttctga
//
LOCUS       DASZZC010000543         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_418519,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000543 DASZZC010000000
VERSION     DASZZC010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418519"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VHE37_11560"
     CDS             <1..946
                     /locus_tag="VHE37_11560"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HVT36217.1"
                     /translation="FRSRYGLTPAGLRRRRGGDVRADIVELKLAYRPPLDWARLLAFL
                     TGRGAAGVELAQDGAYLRTVALGKHRGWLRARPLAGEHALRVEISASLLPVLTPVLAR
                     VRRLFDLDASPQVIEEQLARDARLRAIVRAQPGLRVPGAFDGFELALRAVLGQQVTVK
                     AATTLFGRIAAAYGEPAQTPFAGLKFYSPDAARIAGAGVDAIARIGMPGKRAQTIVQL
                     AREMQRGRLRFEDVALPAQTLERLRGIPGIGDWTANYIAMRALGHPDAWLHSDIGLMQ
                     ALGSRKAKDILAAGEAWRPWRSYAALHLWASLSTTSGG"
     gene            952..1455
                     /locus_tag="VHE37_11565"
     CDS             952..1455
                     /locus_tag="VHE37_11565"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVT36218.1"
                     /translation="MSGEIFYSVMPSPIGELTLVSEGGKLTGLYMESYKHGRARERHW
                     VRDDAKLADARRQLQDYFAGKRTQFDLEMEPAGTAFQKRVWRALCDIPHGETISYGEL
                     ARRIGQPTASRAVGLANGQNPIAVIVPCHRVIGANGSLTGYGGGLPRKQWLLAHERGQ
                     TGGDLFQ"
     gene            1452..2369
                     /locus_tag="VHE37_11570"
     CDS             1452..2369
                     /locus_tag="VHE37_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVT36219.1"
                     /translation="MRTADYVRLLTLAAIWGSSFIFMRVLAPVLGPVVTADARILIAG
                     SVLSLWFAVTGFALDWKREWRQYLVIGAISSALPFMLFAYAAAHIPAGFSAIYNSTAP
                     LFGAVCGALWLGERFTFNRGLGLFAGLAGVALVSRASSSATDEQFAPATLACLGAAAC
                     YALGGVYLKRRASHLKPAMITAGSQLAGGLLLLPLTPMLPPAGGIDATIVLNGLALAL
                     MCSGAAYLIYYRLIADIGPTRALSVTFLIPVFGLLWAFLFLGERITGGMLTGCALVLV
                     GTWLVTRSAAPVQSRPASTVGAMDDEVSG"
     gene            complement(2315..>3862)
                     /gene="nadB"
                     /locus_tag="VHE37_11575"
     CDS             complement(2315..>3862)
                     /gene="nadB"
                     /locus_tag="VHE37_11575"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HVT36220.1"
                     /translation="GAAGLTCALRLAHAGRKIEVVSKGTLASGSTLWAQGGISAVLGA
                     EDSLANHVEDTLVAGAGLCDRAAVRYTVEHGPEAIRWLIEQGVQFTHEGDDPENDLHL
                     TREGGHSHRRVVHAADATGRAVETTLSSLAAAHPNIRVREHTVAVDLIVDRQARRVLG
                     AYLLDARSGRVEAVAAKAVVLATGGANQVYLYSSNPHGASGDGIAMAWRAGCRVENME
                     FMQFHPTTLYHNESRSFLISEALRGEGALLKLPVSGERFMPRFDERAELAPRDIVARA
                     IDHEMKRLGIPYVHLDITHRDASFIRQHFPTIYAHCLTLGLDITREPIPVVPSAHFTC
                     GGVVTDLSARTDVDGLYAIGEVACTGLHGANRLASNSLLECLVFARAAAGDLHQRLDS
                     LAQPGAPRAWDESRVTNSDEDVVVSHNWLELRRFMWDYVGIVRTDKRLERAARRVELL
                     KQEIHDYYGNYKISPGLIELRNLVTVADLIVRSAQARKESRGLHYTLDYPETSSSIAP
                     TVLAGRD"
BASE COUNT          530 a         1298 c         1391 g          643 t
ORIGIN      
        1 gttcaggtcg cgctacggac tgacgccggc gggcctgcgc cgcaggcgtg gcggcgatgt
       61 ccgcgccgac atcgtcgaac tcaagcttgc atatcgcccg ccgctggatt gggcacggct
      121 gctcgcgttc ctcaccggcc gcggtgcggc cggggtcgag ctcgcgcagg acggcgcgta
      181 cctgcgcacc gttgcgctgg gcaagcaccg cggctggctg cgtgcgcggc cgctcgcggg
      241 cgagcacgcg ctgcgggtgg agatttctgc ctcgctgttg ccggtgctga caccggtgct
      301 ggcgcgtgtg cgccggctgt tcgatctcga cgcgtcgccg caggtgatcg aggagcagct
      361 cgcgcgcgat gcgcgcctgc gcgcgatcgt gcgcgcgcaa ccgggcctgc gcgtgcctgg
      421 cgcattcgac ggtttcgaac tggcgctgcg cgcggtgctc ggccagcagg tcacggtgaa
      481 ggcggccacg acattgttcg ggcgcatcgc cgcggcgtac ggcgagccgg cgcagacgcc
      541 gttcgccggg ctgaagttct acagcccgga cgctgcgcgc atcgctggtg cgggcgtcga
      601 cgccatcgcg cgcatcggca tgccgggcaa gcgcgcgcag accatcgtgc agctggcgcg
      661 cgagatgcag cgcggccgcc tgcgcttcga ggatgttgcc ttgcccgcgc agacgctgga
      721 gcggctgcgc gggattcccg gcatcggcga ctggaccgcg aactacatcg ccatgcgcgc
      781 gctgggccat cccgatgcct ggctgcacag cgatatcggc ctgatgcagg cgctcggttc
      841 gcgcaaggcg aaggacatcc tggccgcggg cgaagcctgg cggccctggc gctcatacgc
      901 ggccctgcac ctgtgggcca gcctgagcac gactagcgga ggttgagaaa catgagcggc
      961 gaaattttct acagcgtgat gccgagcccg atcggcgaac tgaccctggt atccgagggc
     1021 ggcaagctga ccggcctgta catggagagc tacaagcacg gccgcgcgcg cgagcggcac
     1081 tgggtgcgcg acgatgcgaa gctggctgat gcgcgccggc agctgcagga ttacttcgcc
     1141 ggcaagcgca cgcagttcga cctggagatg gagcccgccg gcaccgcgtt ccagaaacgc
     1201 gtctggcgcg cgctgtgcga catcccgcac ggcgaaacca tcagctacgg cgaactggcc
     1261 cggcgtatcg gccagccgac ggcctcgcgc gcggtaggcc tggccaacgg tcagaacccg
     1321 atcgcggtga tcgtgccgtg ccaccgcgtg atcggtgcca atggttcgct caccggctac
     1381 ggcggcggcc tgccgcgcaa gcagtggctg ctggcacacg agcgtggcca gaccggtgga
     1441 gacctgttcc agtgaggacg gcagattacg ttcgcttgct cacgctggcc gcgatctggg
     1501 gcagctcgtt catcttcatg cgcgtgctgg cgccggtgct gggcccggtg gtcacggccg
     1561 acgcgcgcat cctcatcgcc ggctcggtgc tgtcgctgtg gtttgcagtc accggattcg
     1621 cgctggactg gaagcgcgag tggcgccaat acctggtcat cggcgcgatc agctccgcgc
     1681 ttccgttcat gctgttcgct tacgcggcgg cgcatatacc cgcgggcttt tcggccatct
     1741 acaactcgac tgcgccgctg ttcggcgccg tatgcggcgc gctgtggctg ggcgagcgtt
     1801 tcacgttcaa ccgcggactg ggactgttcg cggggctggc cggagttgcg ctggtcagcc
     1861 gggcgagcag cagcgcgacc gacgagcagt tcgcgcctgc cacgctcgcc tgtctcggag
     1921 cggcggcgtg ctacgcgctg ggcggcgtgt atctcaagcg gcgtgcctcg cacctgaagc
     1981 cggcgatgat caccgcgggc agccagctgg ccggcggcct gttgctgttg ccgctgaccc
     2041 cgatgctgcc gccggcaggc gggatcgacg cgaccatcgt gctcaacggc ctggctctcg
     2101 ccttgatgtg cagcggtgcc gcctatctca tctattaccg gttgatcgcg gatatcggcc
     2161 cgacgcgagc gctctcggtg acgttcctga tcccggtgtt cggcctgctg tgggcgttcc
     2221 tgttcctggg cgagcgcatc accggcggta tgctcaccgg ctgcgcgctg gtgctggtgg
     2281 gaacctggct ggtgacgcgg tccgccgcgc cagttcagtc gcgcccggcg agcaccgtcg
     2341 gtgcgatgga cgacgaagtc tcgggatagt ccagcgtgta gtgcagtccg cggctttcct
     2401 tgcgcgcctg ggccgagcgc acgatgaggt cggccacggt caccaggttg cgcagctcga
     2461 tcaggccggg gctgattttg tagttgccgt agtagtcgtg gatttcctgc ttgagcagtt
     2521 ccacgcgccg cgcggcgcgc tccaggcgct tgtcggtgcg cacgatgccc acgtagtccc
     2581 acatgaagcg gcgcagctcg agccagttgt gcgacaccac cacgtcctcg tcggaattgg
     2641 tcacgcggct ttcgtcccac gcgcgcggcg cgcccggctg cgccagcgag tcaagccgct
     2701 ggtgcaggtc gccggcggcg gcgcgcgcga acaccaggca ttccagcagc gagttgctgg
     2761 ccaggcggtt ggccccgtgc aggccggtgc aggccacttc gccgatggcg tacagcccgt
     2821 cgacatccgt gcgcgcagac agatccgtga ccacgccgcc gcaggtgaag tgcgccgacg
     2881 gcacgacggg aatcggctcg cgcgtgatgt cgagtcccag cgtcaggcaa tgcgcgtaga
     2941 tggtggggaa gtgctggcgg atgaagctgg cgtcgcgatg ggtgatgtcc aggtgcacgt
     3001 aagggatgcc caggcgcttc atctcgtggt cgatggcgcg cgccacgatg tcgcgcggcg
     3061 ccagttccgc gcgctcatcg aaacgcggca tgaagcgctc gccgctgacg ggcagcttga
     3121 gcagcgcacc ttcgccgcgc agcgcctcgc tgatcaggaa cgagcgcgac tcgttgtgat
     3181 acagcgtggt ggggtgaaac tgcatgaact ccatgttctc gacgcggcag ccggcgcgcc
     3241 aggccatggc gatgccgtcg cccgacgcgc cgtgcgggtt cgacgaatag agatagacct
     3301 ggttggcgcc gccggtggcc agcaccaccg ccttggccgc cactgcctcg acgcggccgc
     3361 tgcgcgcatc gagcaggtag gcgccgagca cccggcgcgc ctggcggtcg acgatcaggt
     3421 cgaccgccac ggtgtgttcg cgcacgcgga tgttcggatg cgctgccgcc agcgaggaca
     3481 gcgtggtttc caccgcgcgg ccggtggcgt cggccgcgtg caccacgcga cggtggctgt
     3541 ggccgccttc gcgtgtcagg tgcaggtcgt tttcggggtc gtcgccttca tgggtgaact
     3601 gcacgccctg ttcgatcagc cagcggatcg cttcaggccc gtgttccacc gtgtagcgca
     3661 ccgccgcgcg gtcgcacagg ccggcgccgg cgaccagcgt gtcctccacg tggttggcca
     3721 gcgagtcctc cgcgccgagc accgccgaga tgccgccctg cgcccatagc gtgctgccac
     3781 tggccagcgt gcccttcgac accacttcaa tcttgcggcc ggcgtgcgcc aggcgcaggg
     3841 cgcaggtgag accggcggcg cc
//
LOCUS       DASZZC010000544         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_47541,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000544 DASZZC010000000
VERSION     DASZZC010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47541"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VHE37_11580"
     CDS             <1..721
                     /locus_tag="VHE37_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36221.1"
                     /translation="PSIAVDVEGGVQLAYYFEGDQVPVRRVIYQRRLPGREFHGVALP
                     LTNTLNKETGWWAQPPVAISIDENAVASVAYFLAGPDHYLQAAYWKPGMPTAIRIAET
                     AQPTQSPEIQIAAGGARANAIFRMAGEDGAAATWLAGGANGGEWAAPLRLPSNAAEGT
                     VAVDVNASGDYAYAISGAPSFVLLRERAGIPVPLPLAAPDAHATQRISLALDDERRLT
                     LAYVDAQDGSLMIYRAALPWD"
     gene            complement(849..2189)
                     /locus_tag="VHE37_11585"
     CDS             complement(849..2189)
                     /locus_tag="VHE37_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVT36222.1"
                     /translation="MNMNFRREAADNLRLALPLLAAQLLFISMGVVDTLMAGRLGGAE
                     LAAIAVGSNVWMQLFVFFMGVCMACSPIVAQREGAGEAPRSIGAFVRGCLPLGLVFGL
                     VWTLTGRVLAWPVISHLGLTPATADLAYRYTLAETWGGPAFALCFVLRSSAEGLGQTR
                     VVLAAGVVALASKIGANILFVHGGAGVPALGVVGLGWGTVASALTMLLSYVAQFSTWR
                     RLRELQVFSGPLRPRPDALELVVLGLPIGLILFAEAAFFCCTALLMARFGDAAVAAHQ
                     IAINVASVSFMAPLSIGFATTVRVGNAMGAGARADALARGRAGMLMGLCFALLSAAVM
                     ALGARGITALYTGVPDVGARAATFLRYAALFQLFDCLQATANGALRGIKDTRVPMLVT
                     ITAYWLVGMPCAWLLAFHFQHGPVALWWGFIIGLALAACGLSLRFLSRAGRTAN"
     gene            2302..2514
                     /locus_tag="VHE37_11590"
     CDS             2302..2514
                     /locus_tag="VHE37_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36223.1"
                     /translation="MFQVYDVRELQRLLRMRRAQALGIFLAGGLFGLLCSQPGVAQPG
                     SSARTAHTPSCEARATFRLDCSLPST"
     gene            complement(2521..>2733)
                     /locus_tag="VHE37_11595"
     CDS             complement(2521..>2733)
                     /locus_tag="VHE37_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVT36224.1"
                     /translation="QVRAGNCYINRGMTGAVVGVQPFGGEGLSGTGPKAGGPHYLLRF
                     VNERTLTINTAAIGGNARLLAQSGEG"
BASE COUNT          468 a          959 c          935 g          371 t
ORIGIN      
        1 cccgtcgatc gccgtcgacg tggaaggcgg cgtgcaactg gcctattact tcgaaggcga
       61 ccaggtgccg gtgcggcgcg tgatctacca gcgccgcctg ccgggcaggg aattccacgg
      121 tgtggcgctg ccgctgacca acacgctgaa caaggaaacc ggctggtggg cgcagccgcc
      181 ggtggcgatc agcatcgacg agaacgcggt ggcgagcgtc gcctatttcc tggccgggcc
      241 cgaccactac ctgcaggccg cgtactggaa acccggcatg ccgacggcga tcaggatcgc
      301 cgagacggcg cagcccacgc agtcgccgga aatccagatc gccgccggcg gagcacgcgc
      361 caacgccatc ttccgcatgg ccggggagga cggcgcagca gccacctggc tggccggcgg
      421 cgccaacggc ggcgagtggg ctgcgccgct gcgcctgccg agcaacgcgg cggagggcac
      481 cgtggcggtc gacgtcaacg ccagcggcga ctacgcctac gcgatctcgg gcgcgccatc
      541 cttcgtgctg ctgcgcgagc gcgccggcat cccggtgccg ctgccgctcg cggcgccaga
      601 cgcgcacgcg acgcagcgca tatcgctggc gctcgacgat gagcggcgcc tcacgctcgc
      661 ctatgtcgat gcgcaggatg gctcgctgat gatctaccgc gcggcgctgc cgtgggattg
      721 acgggatagg ttgcgccggc gaaaaaactg ccaccgcacg cgcatttacg ttgttactaa
      781 aaatataact attttataat tggtaaatat ttagtaacat gccgatcggc ggtgcggtag
      841 gtgttttttc agttcgcggt acgtcccgcg cggctgagaa agcgcagcga caatccgcac
      901 gccgccagcg ccagcccgat gatgaagccc caccagaggg cgaccgggcc gtgctgaaaa
      961 tggaaagcga gcagccatgc gcacggcatg cccaccagcc agtacgccgt gatcgtcacc
     1021 agcatcggca cgcgcgtgtc cttgatgccg cgcagcgcgc cgttggcggt ggcctgcagg
     1081 cagtcgaaca gctggaacag cgcggcgtag cgcaggaaag tggccgcgcg cgcgcccacg
     1141 tcgggcacgc cggtgtacag cgcggtgatg ccgcgcgcgc ccagcgccat caccgccgcc
     1201 gacagcagcg cgaaacacaa tcccatcagc atgccggcgc ggccgcgggc caatgcatcc
     1261 gcgcgtgcgc ccgcgcccat ggcattgccc acgcgcacgg tggtggcgaa gccgatcgac
     1321 agcggcgcca tgaagctcac cgaggcgaca ttgatggcga tctggtgcgc ggccaccgcg
     1381 gcgtcgccga agcgcgccat cagcagcgcc gtgcagcaga agaacgcggc ctcggcgaac
     1441 aggatcagtc cgatcggcag gccgagcacc accagttcga gcgcatcggg ccgcggtcgc
     1501 agcgggccgg aaaatacctg cagctcgcgc aggcgacgcc aggtgctgaa ctgcgcgacg
     1561 tagctcagca gcatggtcag cgcgctggcc accgtgcccc agcccaggcc gacgacaccg
     1621 agcgcgggca cgcccgcgcc gccgtggacg aacaggatgt tggcgccgat cttcgaggcg
     1681 agtgcgacga cgcccgcggc cagcaccaca cgcgtctggc ccagtccttc ggcgctggag
     1741 cgcagcacga agcacagcgc aaaggcgggc ccgccccagg tttccgccag cgtgtagcgg
     1801 tacgccaggt cggccgtggc cggcgtcagg ccgaggtgcg agatcaccgg ccaggccagc
     1861 acgcggccgg tcagcgtcca cacgaggccg aacaccaggc ccagcggcag gcagccgcgc
     1921 acgaatgcgc cgatgctgcg cggcgcctcg cccgcgcctt cgcgctgcgc cacgatcggc
     1981 gagcaggcca tgcacactcc catgaagaac acgaacagct gcatccacac gttggatccg
     2041 accgcgatcg ccgccagctc ggcgccgccc aggcggccgg ccatcagggt gtcgaccacg
     2101 cccatggaga tgaacaacag ctgcgccgcc agcagcggca gtgccaggcg caggttgtcg
     2161 gcggcttctc ggcggaaatt catgttcatg cggatcgccg cgccggcgag ttatccaacg
     2221 gcgcgcgtga agtcttgttg gtcggttggg gatgcgggcg ccgctatcct gcagacatcc
     2281 aggactccag ggagcggggc gatgttccaa gtttacgacg tgcgcgagct gcagcggctg
     2341 ctgcgcatgc gccgtgcgca ggcgctcggc atcttcctcg ccgggggatt gttcggcctg
     2401 ctgtgctcgc agccgggcgt tgcgcaaccc ggcagcagcg cgcgcaccgc gcataccccg
     2461 agctgcgaag cgcgcgccac gttcaggctg gactgcagcc tgccgtcgac ctgaacaatt
     2521 ttagccctcg ccgctctgtg ccagcagccg cgcgttgcca ccgatggccg cggtgttgat
     2581 ggtcagtgtg cgctcgttga cgaagcgcag caggtagtgc ggcccgcccg cctttggccc
     2641 cgttcccgac aggccctcgc ccccgaacgg ctgcacgccg accaccgcgc cggtcatgcc
     2701 gcggttgatg tagcagttgc ccgcgcgcac ctg
//
LOCUS       DASZZC010000545         7655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1138950,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000545 DASZZC010000000
VERSION     DASZZC010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138950"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..871)
                     /gene="pssA"
                     /locus_tag="VHE37_11600"
     CDS             complement(95..871)
                     /gene="pssA"
                     /locus_tag="VHE37_11600"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HVT36225.1"
                     /translation="MEPTLTSTGRRRRGIYWLPNLFTTGTLFGGFYAIVASMRGDYGN
                     ACVAILAAMLFDTFDGYIARKTNTASDFGKEYDSLCDMVAFGLAASLVMYNWSLHYLS
                     EYKWLGGKLGWVICFIYTSCAALRLARFNVLASRGEAKGAFFGLPSPGAAAVVSGLVW
                     ASDASGLNPETMVIPAAIITLGAAFLMISNMRFFSLKMLNFGQRIPFRYMVITVGILG
                     LFAVDPPRIFFLFFFLYALTGPVGAIVRRVRKPPAAVQAK"
     gene            complement(961..1977)
                     /gene="ilvC"
                     /locus_tag="VHE37_11605"
     CDS             complement(961..1977)
                     /gene="ilvC"
                     /locus_tag="VHE37_11605"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HVT36226.1"
                     /translation="MTVYYDKDADLSIIQGKKVTILGYGSQGHAHALNLKDSGVDVTV
                     ALRKSSKSWAKAENAGLKVKEVAEAVKTADLVMILAPDQDQKKIYEESVAPNLKKGGA
                     VAFAHGFNIHFQLIEPRADLDVIMIAPKGPGHTVRSTYTQGGGVPSLIAIHQDATGNA
                     KKIALSYASANGGGRAGIIETNFREETETDLFGEQAVLCGGASALVQAGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGISTMRYSISNTAEYGDYVTGPRIVTEETKKEMKR
                     VLNDIQTGKFARDFVAENLAGQPVLKARRRLGAEHQIEQVGAKLRAMMPWIAKNKLVD
                     KTKN"
     gene            complement(2039..2539)
                     /gene="ilvN"
                     /locus_tag="VHE37_11610"
     CDS             complement(2039..2539)
                     /gene="ilvN"
                     /locus_tag="VHE37_11610"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HVT36227.1"
                     /translation="MRHIISILLQNEAGALARVAGMFASRGYNIESLTVAPTHEPTVS
                     RLTLVSIGSDAVIEQIIQQTRKLVDVVEITDLSARDHLETEMLMLKVQADAASARTVI
                     DCVRRHRGQMLDETDDTRMIQLAGTGAEVDRFIAEIGKLATILELVRSGVAALERGKR
                     VLALPA"
     gene            2623..3156
                     /locus_tag="VHE37_11615"
     CDS             2623..3156
                     /locus_tag="VHE37_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36228.1"
                     /translation="MKNVAVALLLACLAGAADADHDCSTGAIGGAQSKTSAPEQATPC
                     ILMGRMFCRVALMRNENVPMERAIGDSVDWFNRLKQTGSHLTRIDYRPLATGVAQYLY
                     AHPPALGWTDYYYGVYTCGLDQRLPATGDKSAAMQRWERAAQDCEQQHPGAGDGYVND
                     ELRACFDGAMKDIAAAR"
     gene            3217..5043
                     /gene="ilvB"
                     /locus_tag="VHE37_11620"
     CDS             3217..5043
                     /gene="ilvB"
                     /locus_tag="VHE37_11620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HVT36229.1"
                     /translation="MNAVLKTHAHPLAGKTMSGAEIVVQVLADEGVDVIFGYSGGAIL
                     PTYDAVFRFNAANNEKMPLIVPANEQAAGFMAAGYARASGKVGVCLVTSGPGATNTVT
                     PVRDCMADSIPIVVISGQVPVPAIGSDGFQEAPIANIMSACAKHVFLVTDAKKLEATM
                     RTAFEIARSGRPGPVVVDIPKDVQNWSGPYQGEGALPVPGYRARLNAVTGNRLDDAAA
                     QKFFDILSQAKKPLIYAGGGVINGEATAELRDFADSFGIPVVTTLMGIGAVDTTAQLS
                     LQMLGMHGTAYANYATDDCDFLIAVGSRFDDRVAGVPGKFARSARWIAHFDIDPSEIG
                     KVKRVHWSHVGELKQALAAITAHARARKFAPEWSAWHAHVGELKKRHAMNYERGGSAI
                     QPHAVIEEINRITKGEAIVATGVGQHQMWAAQYFDFKQPRLWLTSGSMGTMGFGLPAA
                     IGAAFAAKGRTVIDIDGDASIRMNLGELETATTYNLPVKIVVLNNFGDGMVKQWQRLF
                     FESRYAGSDKSLHRKDFIKAAQADGYQYARRVEKKEELAAAVREFIAHPGPSFLEVII
                     DPMASVYPMVGPGMSYEQMITGDWIPSRGTASTEKSDKADLF"
     gene            complement(5047..6513)
                     /locus_tag="VHE37_11625"
     CDS             complement(5047..6513)
                     /locus_tag="VHE37_11625"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT36230.1"
                     /translation="MIRSAIATCLLLLLCACGGGGAGARSDHPAVDTSGGAVGASPGS
                     SDAGSDPGSGAGSDEGSGESPGGSTGADGGGSTGSGIAAPVISAFSADASAINPGRSV
                     TLSWNTSGAEQLLLMPGNIDVTGTTTHQLTPGASTIYTLLAHNAGGDTQAQLPVRLYD
                     WSQLAATLDSFLAPGGALVGYSFLLFDRNGSLYARAGGNQDMGSVESIASATKLASAA
                     AIMTLVDAGKLELDRPVGEYFRLGTIVWPLDKAGITTRMLLNHTSGLPGFQDRNQPTC
                     INLESTTTLQKCAQDIAGAALLYPPGTTYDYGGADYQVAGYLATVISGKNWQQFFADA
                     IGTPLNMSSFTYGDPAKITNPRIAGGADSDAADYAQLLAMVQGNGAVGGVRVLSAAAV
                     AELQRNQIAGKPVAYSPLDPAQYPGYSYGLFASSASLYAGSPGPEFSDPGLLGTTPWI
                     DAGLGYGAVMLTTSNTQTGLNLWNAVRPQIISQLTGAP"
     gene            6626..7633
                     /locus_tag="VHE37_11630"
     CDS             6626..7633
                     /locus_tag="VHE37_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVT36231.1"
                     /translation="MKNRQFKLAQRPQGMVKASDFEFVESAVGEPQDGEVLTRILYIS
                     LDPAMRGWMNEGKSYIPPVGLGEVMRAGTLGQIVASKNPGYKAGDYVVGTDGVQDYAI
                     SNTKGYAKVDAKLAPLPKYLGVLGMPGLTAYFGLLEVGQPKAGETIVVSGAAGAVGQV
                     VGQIGKILGCRVIGIAGGADKCEFVVKELGFDACIDYKHDDVKKALKKAAPEGIDVYF
                     DNVGGDILDAVLTQLRMKARVVICGAISQYNNMTAVKGPSNYMSLLVNRARMEGMVVF
                     DWAKRYGEGIAQMAQWMAQGKLKSREHVVEGLKTFPETLQMLFTGENTGKLVLKVDHA
                     N"
BASE COUNT         1339 a         2438 c         2566 g         1312 t
ORIGIN      
        1 gaactgcttt atgccgtcgg ctgcctgcgc gcgcggcagg agcagcaggc aggccagtgc
       61 gagcgtgcgg agcagcgtcg cgccgcgcaa cgagctactt cgcctgcacc gcggcgggcg
      121 gcttgcgcac acggcgcacg atggcgccca ccggaccggt cagcgcgtag aggaagaaga
      181 acaggaaaaa gatgcgaggc gggtcgacgg cgaacagtcc gaggatgccg accgtgatga
      241 ccatgtaccg gaatgggatg cgctggccga agttgagcat cttcaggctg aagaagcgca
      301 tgttgctgat catcagaaac gccgcgccca gcgtgatgat ggctgccggg atgaccatgg
      361 tctccgggtt cagcccgctg gcatccgacg cccacaccag cccgctcacc accgccgccg
      421 cgccggggct gggcaggccg aagaacgcgc ccttggcttc accgcgcgag gccagcacgt
      481 tgaagcgtgc caggcgcagc gctgcgcacg aggtgtagat gaagcagatc acccagccca
      541 gcttgccgcc cagccacttg tactcggaca ggtagtgcag gctccagttg tacatgacca
      601 gcgaagcggc gagcccgaat gccaccatgt cgcacagcga gtcgtattcc ttgccgaaat
      661 cgctggcggt gttggttttg cgcgcgatgt agccgtcgaa ggtgtcgaac agcatggcgg
      721 caaggatggc gacgcaggcg ttgccgtagt caccgcgcat cgaggcgacg atggcataga
      781 agccgccaaa cagggtgccg gtggtgaaca ggttcggcag ccagtagatg ccgcggcggc
      841 ggcggccggt ggatgtcagg gtcggttcca tgttttgttt gacctctgcc gtggagttta
      901 cgtttgcgcc gctaaaaaaa taagggcgcc gaacggcgcc cttatgacaa cgtccaatgg
      961 tcagttcttg gtcttgtcga ccagcttgtt cttggcaatc cacggcatca tcgcgcgcag
     1021 ctttgcaccc acctgttcga tctggtgctc ggcgcccagg cggcggcgtg ccttgagcac
     1081 cggctggccc gccaggttct cggcgacgaa gtcgcgggcg aacttgccgg tctggatgtc
     1141 gttgagcacg cgcttcattt ccttcttggt ttcctcggtg acgatgcgcg ggccggtcac
     1201 gtagtcgccg tactcggcgg tgttggagat cgagtagcgc atggtggaga tgccgccctc
     1261 gtacatcagg tcgacgatca gcttgagttc atgcaggcac tcgaagtacg ccatttccgg
     1321 cgcgtagccg gcttccacca gcgtctcgaa gcccgcctgc accagcgcgc tggcgccgcc
     1381 gcagagcacg gcctgctcgc cgaagagatc ggtctcggtc tcctcgcgga agttggtttc
     1441 gataattccg gcgcggccac cgccgttggc gctggcatac gacagcgcga tcttcttggc
     1501 gttgccggtg gcgtcctggt ggatcgcgat caggctgggc acgccgccgc cctgtgtgta
     1561 ggtgctgcgc acggtgtggc cggggccctt gggcgcgatc atgatcacgt cgaggtcggc
     1621 gcgcggctcg atcaactgga agtggatgtt gaagccgtgc gcgaaagcga ccgccccgcc
     1681 cttcttcaag ttgggcgcca cgctttcttc gtagatcttc ttctggtcct ggtccggcgc
     1741 gaggatcatg acgaggtcgg cggtcttcac cgcctcggcg acttccttga ccttgaggcc
     1801 ggcgttctcc gccttggccc agctcttgga gctcttgcgc agcgcgacgg tgacgtcgac
     1861 accgctgtcc ttgaggttca gcgcgtgggc atggccctgc gagccgtagc ccaggatagt
     1921 gaccttcttg ccctggatga tggaaaggtc tgcgtccttg tcgtagtaca ccgtgattgc
     1981 cattgcttaa ctccgctcac tcactgttga acgggacctg gcggccccgc actaaaaatc
     2041 aggccggcaa ggccagcacc cgcttgccgc gttccagcgc tgcgacgccg ctacgcacca
     2101 gttcgaggat ggtcgccagc ttgccgatct cggcgatgaa gcggtccacc tcggcgccgg
     2161 tgccggcaag ttggatcatg cgcgtgtcgt cggtctcgtc gagcatctgg ccacgatgcc
     2221 ggcgcacgca gtcgatcacc gtgcgggccg atgctgcgtc cgcctgcact ttcagcatca
     2281 gcatctcggt ttcgaggtga tcacgcgccg acaggtcggt gatctccacc acgtcgacca
     2341 gcttgcgggt ctgctggatg atctgctcga tcaccgcgtc gctgccgatg ctgaccagcg
     2401 tgagccgcga gacggtcggc tcgtgcgtgg gagcgacggt cagcgattcg atgttgtagc
     2461 cgcggctggc gaacatgccg gccacgcggg ccagcgcgcc ggcctcgttt tgcagcagga
     2521 tcgagatgat gtgtcgcata ggggtgcgca ccttaatgag gttgctgctg cggtgcaata
     2581 gcagcgcagc gatcgtctac catcgctcca aaaggagagc ccatgaagaa cgtcgccgtt
     2641 gcgctgctgc tggcgtgcct ggcgggcgcc gccgatgccg accatgactg ttccaccggc
     2701 gccatcggcg gggcgcagag caagacctcc gcgccggaac aggcgacgcc ctgcatcctg
     2761 atggggcgca tgttctgccg ggtggcgctg atgcgcaacg agaatgtgcc gatggagcgc
     2821 gccatcggcg acagtgtcga ctggttcaac cggctcaagc agaccggatc gcacctgacc
     2881 aggatcgact accggccgct ggccacgggc gtcgctcaat atctgtatgc gcacccgccg
     2941 gccttgggct ggaccgacta ttactacggc gtctacacct gcggcctcga ccagcgcctg
     3001 ccggcgacgg gagacaaatc cgcggcgatg cagcggtggg aacgggctgc gcaggattgc
     3061 gagcagcaac accctggcgc cggcgacggc tatgtcaatg acgaactgcg cgcgtgcttc
     3121 gacggagcga tgaaggacat cgcggcggcg cgctgaggag tttccctcat ggaggtgtag
     3181 aattgcgccc cctccacgca ccgacgaagt atcgccatga acgctgtgct caagacccat
     3241 gcccatcccc tcgcaggcaa gaccatgtct ggcgcggaga ttgtggtaca ggtcctggcc
     3301 gacgaaggcg tggatgtcat cttcggctac tcgggcggcg ccatcctgcc gacttacgac
     3361 gcggtgttcc gtttcaacgc cgccaacaac gagaagatgc cgctgatcgt gccggccaac
     3421 gagcaggccg ccgggttcat ggcggcgggc tatgcgcgcg cttcgggcaa ggtgggcgtc
     3481 tgcctggtca cttccggccc cggcgccacc aacacggtga cgccggtgcg cgactgcatg
     3541 gccgactcaa ttcccatcgt ggtcatcagc ggccaggtgc cggtgccggc catcggcagt
     3601 gacggcttcc aggaagcgcc catcgccaac atcatgtcgg cctgcgccaa gcatgtgttt
     3661 ctggtcaccg atgcgaagaa gctcgaagcg accatgcgca ccgcgttcga gatcgcgcgc
     3721 agcggccgtc ccgggccggt ggtggtcgat attcccaagg acgtgcagaa ctggagcggc
     3781 ccttaccagg gtgaaggcgc cctgcccgtc cctggctacc gcgcgcgcct caatgcggtg
     3841 accgggaacc gtctcgacga tgcggccgcg cagaagttct tcgacatcct gtcccaggcg
     3901 aagaagccgc tgatctatgc cggcggcggc gtgatcaacg gcgaggccac cgcggagctg
     3961 cgcgatttcg ccgacagctt cggcatcccg gtggtgacca cgctgatggg tatcggcgcg
     4021 gtggatacca ccgcgcagct gtcgctgcag atgctgggga tgcatggcac cgcctacgcc
     4081 aactacgcca ccgacgactg cgacttcctg atcgcggtgg gctcgcgctt cgatgaccgc
     4141 gtggccggcg tgccgggcaa gttcgccagg agcgccaggt ggatcgcgca cttcgacatc
     4201 gacccgtcgg agatcggcaa ggtcaagcgc gtgcactggt cgcacgtggg cgagctgaag
     4261 caggcgctgg cggcgatcac cgcccacgcg cgcgcacgca agttcgcgcc ggagtggagc
     4321 gcgtggcacg cccacgtggg cgagctcaag aagaggcacg cgatgaatta cgagcgcggc
     4381 ggcagcgcca ttcagccgca cgcggtgatc gaggagatca accgcatcac caagggcgaa
     4441 gccatcgtcg ccaccggcgt gggccagcac cagatgtggg ccgcacagta tttcgatttc
     4501 aagcaaccgc gcctgtggct cacctcgggt tcgatgggca ccatgggctt tggcctgccg
     4561 gccgccattg gcgcggcgtt cgccgccaag ggccgcaccg tcatcgacat cgacggcgac
     4621 gcctccatcc gcatgaacct gggcgaactg gagacggcta ccacctacaa cctgccggtg
     4681 aaaatcgtgg tgctgaacaa cttcggcgac ggcatggtca agcagtggca gcggctgttc
     4741 ttcgagagcc gctacgccgg ctcggacaaa tcgctgcacc gcaaggactt catcaaggcc
     4801 gcgcaggccg acggctacca gtacgcgcgc cgcgtggaga aaaaggaaga actggccgcg
     4861 gccgtgcgcg agttcatcgc gcatccgggc ccgtccttcc tggaagtgat catcgacccg
     4921 atggcctcgg tctacccgat ggtcggcccc ggcatgagct acgagcagat gatcaccggc
     4981 gactggattc cctcgcgcgg aacggccagc acggaaaagt cggacaaggc cgacctgttc
     5041 tgaacgctac ggagcgccgg tcagctggct gatgatctgc ggccgcaccg cgttccacag
     5101 gttgaggccg gtctgcgtgt tgctggtggt gagcatcacc gcgccgtagc cgagaccggc
     5161 gtcgatccac ggcgtggtgc cgagcaggcc ggggtcactg aactccgggc cgggactgcc
     5221 cgcatagagc gacgcgctcg aagcgaacag gccgtagctg tagccgggat actgcgctgg
     5281 atcgagcggc gaataggcca ctggtttgcc ggcgatctgg ttgcgctgca gttcggccac
     5341 cgccgcggcg ctcagcacgc gcaccccacc gaccgctccg ttgccctgca ccatcgccag
     5401 cagctgcgcg tagtcggcgg catcgctgtc ggcgccgccg gcgatgcgcg ggttggtgat
     5461 cttggccggg tcgccgtagg tgaaactgct catgttcagc ggcgtgccga tggcatcggc
     5521 gaagaactgc tgccagttct tgccgctgat caccgtcgcc aggtagccgg ccacctggta
     5581 atcggcgccg ccatagtcgt aggtggtgcc cggcggatac agcaacgccg ctccggcgat
     5641 gtcctgcgcg catttctgca gcgtggtggt gctctccagg ttgatgcagg tgggctggtt
     5701 gcgatcctgg aacccgggca ggccggaggt gtggttgagc agcatgcgcg tggtgatgcc
     5761 ggccttgtcc agcggccaga cgatggtgcc gaggcgaaag tactcgccca ccggccggtc
     5821 cagttccagc ttgccggcgt cgaccagcgt catgatcgcg gcggccgatg ccagcttcgt
     5881 ggcagacgcg atcgactcca ccgagcccat gtcctggttg ccgccggcac gcgcgtacag
     5941 cgatccgttg cggtcgaaca gcaggaagct gtacccgacc agcgccccgc cgggcgcaag
     6001 aaagctgtcc agcgtagctg ccagctgtga ccagtcgtac agccgcaccg gcagttgcgc
     6061 ctgcgtgtca ccaccggcgt tgtgcgccag cagcgtgtag atggtgctgg cgcccggcgt
     6121 gagttgatgc gtcgtggtgc cggtgacgtc gatgtttccc ggcatcagca gcaattgttc
     6181 ggcgccactg gtgttccacg acagcgtcac gctgcggccc ggattgatcg ccgatgcatc
     6241 cgcgctgaac gcgctgatca ccggcgcggc gatgccgctg cccgtcgagc caccaccatc
     6301 ggcaccggtg ctgccgcccg gactttcgcc ggacccctca tcagaacctg caccggagcc
     6361 tgggtcggaa cctgcatcgg agcttccagg cgatgcgccg acggcaccgc cgctggtatc
     6421 caccgctggg tgatcgctgc gcgcacccgc gccaccaccg ccacaggcgc agagcaggag
     6481 caacaggcag gtggcaatgg cgctgcggat catggcgcgc aagtttaatg agacggacag
     6541 gctgactggg tgccgcagcg atggtaatga ttcattcctg cagctttgtg gcacgctagc
     6601 cgcccctttt cgccggacag cacagatgaa gaaccgccag ttcaaactcg cgcagcgccc
     6661 gcagggcatg gtcaaggcca gcgatttcga attcgtcgaa tcggccgtcg gcgagccgca
     6721 ggacggcgaa gtgctgacca ggatcctgta catctcgctc gacccggcga tgcgcggctg
     6781 gatgaacgaa ggcaagtcgt acattccccc tgtgggcctg ggcgaagtga tgcgcgcggg
     6841 cacgctcggc cagatcgtcg cgtcgaagaa tcccggctac aaggccggcg actacgtggt
     6901 cggcaccgac ggcgtgcagg actacgccat ctccaacacc aagggctatg ccaaggtgga
     6961 tgccaagctg gcgccgctgc ccaagtacct gggcgtgctc ggcatgcctg gcctcaccgc
     7021 ctattttggc ctgcttgaag ttggccagcc gaaggccggc gagaccatcg tggtatccgg
     7081 cgcggcgggc gccgtcggcc aggtagtggg ccagatcggc aagattctcg gctgccgcgt
     7141 gattggcatc gccggcggcg ccgacaaatg cgagttcgtg gtgaaggaac tcggcttcga
     7201 cgcctgcatc gactacaagc atgacgacgt gaagaaggcg ctgaagaaag ccgcgccgga
     7261 aggcatcgac gtgtacttcg acaatgtagg cggcgacatt ctcgacgccg tgctgacgca
     7321 actgcgcatg aaggcgcgcg tggtgatctg cggcgccatc agccagtaca acaacatgac
     7381 cgcggtgaaa ggcccgtcta attacatgtc gctgctggtc aaccgcgcgc gcatggaagg
     7441 catggtggtg ttcgactggg ccaagcgcta cggcgaaggc atcgcgcaga tggcgcagtg
     7501 gatggcgcag ggcaagctga aatcgcgcga gcacgtggtg gaaggattga agaccttccc
     7561 cgagacgctg cagatgctgt tcaccggcga gaacaccggc aagctggtgc tcaaggtcga
     7621 ccacgccaac tgatcaaccc aggaaaattg cggag
//
LOCUS       DASZZC010000546         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_810861,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000546 DASZZC010000000
VERSION     DASZZC010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810861"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..1878
                     /gene="trkA"
                     /locus_tag="VHE37_11635"
     CDS             502..1878
                     /gene="trkA"
                     /locus_tag="VHE37_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HVT36232.1"
                     /translation="MKIIILGAGQVGATLAENLANEQNDVTVVDSDPSLLEELRERLD
                     IATVQGHAGHPSTLRRAGADDADMLIAVAESDETNMLACRVAQVLFHTPTKIARVRSS
                     EYLSEEKLFGEDGLSVDLHISPEQLVTDYITRLIEFPGALQVVDFAGGRVRLVGVRAY
                     YGGLLVSRQLKELPRHLPEGVEARVAAIYRRGRPILPEGDTVIEADDEVFFVAASEHI
                     PKIMGELRRLDKPVKRVMLAGGGNIGLRLAHALEGKLQVKLIERARERARVLAEELNT
                     AVVLHGDAANEALLREENIEDVDVFCAVTNDDEANILSAMLAKQLGAEKALCLINRVS
                     YVDLVEGGAIDIAISPQQSTVSALLAHVRRGDVVKVHTLRRGAAEAIEAVAHGDRHNS
                     KVVGRRLDEIKLPPGTTIGAVVRGDEVIIAHHDTLIQAEDHVILFIVDKQYTKDVERL
                     FQVGLKFF"
     gene            1885..>3213
                     /locus_tag="VHE37_11640"
     CDS             1885..>3213
                     /locus_tag="VHE37_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HVT36233.1"
                     /translation="MPQPQHATLARRHAAVQRIMGILLMLFSATMLPPMLVDLVYEGS
                     MFGAFGKSLAITLLTGALIWLPVRDNRVELKTREGFLITVLFWVVLSLFGAIPLYTSG
                     RAWYTYTDALFETVSGLTTTGATVVASGLDALPHALLYYRAQLHWFGGMGIIVLAVAV
                     LPMLGIGGMQLYKAEAPGPVKDAKLTPRITGTARALWLVYVSLTAACALTYWLLGMNL
                     FDAVCHAFSTLATGGFSTHDASIGYFDSVAIEWACILFMLAGASNFGLHYLAFEQRSL
                     SGYLRDTEFRTFLALIAVMGVLVCVPLYLLQVYPFLGTSLRKGFFQLVAYATNTGFRT
                     ADPSAWPGFIPMLLVLATFMVSCAGGTGGGVKAVRLVLFVKQAGRELKRLVHPNAEVA
                     IKLDGKVVDNDVVYAIGGFFSVYIGMTIVLTFLMMATGLDAVTAFSAVAAC"
BASE COUNT          513 a         1068 c         1076 g          556 t
ORIGIN      
        1 gtgctcacgc cggtggtgtt gccgccggtg ctgtcgccgc cggcgctgct gccggtggcg
       61 gagccgctgc cgccggaggt gggatcggaa gtgctgcagg ccgcgaccag gcacggcaag
      121 gcaagcgcga gcagcagcgt acgcggcgtg accctcatgg aaatctcccc cacggtctct
      181 tcgaaaaaaa tgtcggtgcg ggccgtgcgc gccgccggcc tcttgtgatg tgcagggatt
      241 ctaggcgttg tcatccggcg gcaatagagt aattttcaac tgagaacttg cccgtgcagt
      301 gtccaccgca ccgggcctgg cgacgttata aaatatcgac tatatttata aataatagat
      361 aattaataac atcaaaaata caggtgcggt caggtgctta gcccggcaaa aaagcagttt
      421 ggagacgtgg tttgttgcgc ctgccggacg cgcggcgtaa ggtgcgcgcc ccatgaaccg
      481 gccgtttaat acgacaggcg cgtgaagatc atcatcctcg gcgccggcca ggtcggagcc
      541 acgctggcgg aaaatctcgc caacgaacag aacgatgtca ccgtcgtcga cagcgacccc
      601 tcgctgctcg aggaactgcg cgagcgcctg gacatcgcca ccgtgcaggg ccacgccggc
      661 cacccgagca cgctgcgccg cgccggcgcc gacgatgccg acatgctgat cgcggtggcc
      721 gagtccgacg agaccaacat gctcgcctgc cgcgtggcgc aggtgttgtt ccacacgccg
      781 accaagatcg cgcgcgtgcg ctcgtcggaa tacctgtcgg aagaaaaact gttcggcgag
      841 gatggcctgt cggttgacct gcacatctcg cccgagcagc tggtcaccga ctacatcacc
      901 cgcctgatcg aatttcccgg cgcgctgcag gtggtggatt tcgccggcgg ccgcgtgcgc
      961 ctggtcggcg tgcgcgccta ctacggcggg ctgctggtgt cgcgccagct caaggaactg
     1021 ccgcggcacc tgcccgaagg cgtggaggcg cgcgtggccg ccatctaccg ccgcgggcgg
     1081 ccgatcctgc ccgagggcga cacggtgatc gaggccgacg acgaggtgtt tttcgtggcc
     1141 gcctccgagc acattccgaa aatcatgggt gagctgcgcc ggctggacaa gccggtcaag
     1201 cgcgtcatgc tggccggcgg cggcaacatc ggcctgcgcc tggcgcacgc gctggaaggc
     1261 aagctgcagg tgaagctgat cgagcgcgcg cgcgagcgcg cccgcgtgct ggcagaggag
     1321 ctcaacaccg cggtggtgct gcacggtgac gctgccaacg aggcgctgct gcgcgaggag
     1381 aacatcgagg acgtcgacgt gttctgcgcg gtgaccaacg acgacgaggc caacatcctg
     1441 tcggccatgc tggccaagca gctcggcgcg gaaaaggcgc tgtgcctgat caaccgcgtg
     1501 tcctacgtgg acctggtgga gggcggcgcc atcgacatcg ccatctcgcc gcagcagtcc
     1561 accgtgagcg cgctgctggc gcacgtgcgc cgcggcgacg tggtcaaggt gcacacgctg
     1621 cgccgcggcg cggccgaggc gatcgaggcg gtggcgcacg gcgaccgcca caactccaag
     1681 gtggtcggcc ggcggctgga cgagatcaag ctgccgccgg gcaccaccat cggcgcggtg
     1741 gtgcgcggcg acgaggtgat catcgcccac cacgacacgc tgatccaggc cgaggatcac
     1801 gtcatcctgt tcatcgtcga caagcagtac accaaggatg tcgagcgcct gttccaggtc
     1861 ggcctgaagt ttttctaggc gacgatgccg cagccgcaac atgccacgct tgcgcgccgc
     1921 cacgcggcag tgcagcgcat catgggcatc ctgctgatgc tgttctccgc caccatgctg
     1981 ccgccgatgc tggtggacct ggtctacgag ggcagcatgt tcggggcctt cggcaagagc
     2041 ctggccatca ccctgctcac cggcgcgctg atctggctgc cggtgcgcga caaccgggtg
     2101 gagctgaaga cgcgcgaagg cttcctcatc accgtgctgt tctgggtggt gctgtcgctg
     2161 ttcggcgcca ttccgctcta cacctccggc cgcgcctggt acacctatac cgacgcgctg
     2221 ttcgagacgg tgtcggggct caccaccacc ggcgcgacgg tcgtggccag cgggctcgac
     2281 gcgctgccgc acgcgctgct gtattaccgc gcgcagctgc actggttcgg cggcatgggc
     2341 atcatcgtcc tcgccgtggc ggtgctgccc atgctcggca tcggcggcat gcagctgtac
     2401 aaggccgagg cgcccggccc ggtgaaggac gccaagctca ccccgcgcat caccggcact
     2461 gcgcgcgcgc tgtggctggt ctacgtgtcg ctcaccgccg cctgcgcgct cacctactgg
     2521 ctgctgggca tgaacctgtt cgacgcggtg tgccatgcgt tctcgacgct ggccaccggc
     2581 ggcttctcca cgcatgacgc gtcgatcggc tacttcgaca gcgtggccat cgagtgggcc
     2641 tgcatcctgt tcatgctggc cggcgcctcc aacttcggcc tgcactacct cgcgttcgag
     2701 cagcgctcgc tgtccggcta cctgcgcgac accgagttcc gcaccttcct cgctttgatc
     2761 gcggtgatgg gcgtgctggt gtgcgtgccg ctgtacctgc tgcaggttta tccatttctc
     2821 ggcacctcgc tccgcaaggg cttcttccag ctggtggcct acgccaccaa caccggcttc
     2881 cgcacggcgg acccgagcgc gtggcccggc ttcatcccga tgctgctggt gctggccacc
     2941 ttcatggtgt cgtgcgccgg cggcaccggc ggcggggtca aggcggtgcg gctggtgctg
     3001 ttcgtcaagc aggccgggcg cgagctcaag cgcctggtgc atcccaacgc cgaggtggcg
     3061 atcaagctcg acggcaaggt ggtggacaac gacgtggtct acgccatcgg cggattcttc
     3121 tcggtgtaca tcggcatgac catcgtgctc accttcctga tgatggccac cgggctcgac
     3181 gcggtgaccg cgttctccgc ggtggcggcg tgc
//
LOCUS       DASZZC010000547         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1198695,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000547 DASZZC010000000
VERSION     DASZZC010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198695"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..728)
                     /locus_tag="VHE37_11645"
     CDS             complement(153..728)
                     /locus_tag="VHE37_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36234.1"
                     /translation="MEADMQTQAPAPAAAKPYDLFAERSIKLLLVLFFGALFYYLADN
                     YVVLSYQPLEQIPLPLFAAAALLGAAAAGWLARKAPLTEHVALSVMCAIVAWLAAQPL
                     LPRINAATASGAQTAGYVQQRDGAWHSAGLPDVRLDRRHANLAAGMTLTSRTLQFHLW
                     RGMLGFYQLDLGALRSDYADANSNWSGAAKP"
     gene            complement(728..1435)
                     /gene="dnaQ"
                     /locus_tag="VHE37_11650"
     CDS             complement(728..1435)
                     /gene="dnaQ"
                     /locus_tag="VHE37_11650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HVT36235.1"
                     /translation="MSRQIVLDTETTGLEVSQKHRVIEIGCVELKNRRPTGNNFHRYV
                     NPQREIDAGAEQVHGITSQFLADKPLFGAFAREFWEYLHGAELIIHNAEFDLGFLNYE
                     FELASVGQRLHDVCSVIDTVTLARKLHPGQKVNLDALCKRYNVDNSNRDFHGALLDAR
                     LLADVYLAMTGGQSRLTLDAEQSAAGQSRFVELLGKSSAPLPVLAASDEELAAHRARM
                     AEIGKKAGKLVWMEESA"
     gene            complement(1432..1590)
                     /locus_tag="VHE37_11655"
     CDS             complement(1432..1590)
                     /locus_tag="VHE37_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HVT36236.1"
                     /translation="MWQQLEAAAARHRIEWKWVKGHAGDPGNELADALANEGIDEMAA
                     LAAGAGEK"
BASE COUNT          245 a          563 c          503 g          285 t
ORIGIN      
        1 tcacgccaat ctcggcacca cgcccgacaa gctgcgcaag ctgctgaccg gcgccggctt
       61 cagcgtggag atgtgccgcg tgacctcgcg cgaatcaaag ccgccctact tcgaagtggt
      121 gaccgcgctg gcgcaatcgg cctagcgacc cttcaaggct tggccgcgcc gctccagttg
      181 ctgttggcgt ctgcgtagtc gctgcgcaac gcaccgagat ccagctggta gaaaccgagc
      241 atgccgcgcc acaggtggaa ctgcagcgtc ctgctggtca gcgtcatgcc ggcggcaagg
      301 ttggcgtggc gccggtcaag acggacgtca ggcagccccg ccgaatgcca ggcgccatcc
      361 cgctgttgca cgtagcccgc ggtctgcgct ccgctggccg tcgctgcgtt gatccgcggc
      421 agcagcggct gcgccgccag ccaggcgacg atcgcgcaca tcacgctcaa cgcgacatgc
      481 tcggtcagcg gcgccttgcg tgccagccag ccggccgctg cggcacccag cagcgcggct
      541 gcggcaaaca gcggcaacgg gatctgttcg agcggctggt acgacagcac gacgtagttg
      601 tcagccaggt aataaaacag tgcgccgaaa aacagcacga gcagcagctt gatcgaacgc
      661 tccgcgaaca ggtcgtatgg tttggcggcg gccggggccg gcgcctgcgt ttgcatatcc
      721 gcttccatca cgcgctctcc tccatccaga ccagcttgcc cgctttcttg ccgatctccg
      781 ccatgcgcgc acggtgcgcg gccagctctt catcgctcgc cgccagcacc ggcagcggcg
      841 cgctgctctt gcccagcaac tcgacgaagc gcgactggcc ggccgcggac tgctcggcgt
      901 ccagcgtcag ccgcgactgc ccgccggtca tggccaggta gacgtcggcc agcaggcgcg
      961 cgtcgagcag cgcgccgtgg aaatcgcggt tggagttgtc gacgttgtag cgcttgcaca
     1021 gcgcgtccag gttgaccttc tgcccggggt gcagcttgcg cgccagggtc accgtgtcga
     1081 tcacgctgca tacgtcgtgc agccgctggc cgacgctggc cagttcgaac tcgtagttga
     1141 ggaagcccag gtcgaactcg gcgttgtgga tgatcagctc ggcgccgtgc aggtactccc
     1201 agaattcccg cgcgaacgcg ccgaacagcg gcttgtcggc caggaactgg ctggtgatgc
     1261 cgtgcacctg ctcggcgccg gcgtcgatct cccgctgcgg gttgacgtag cggtgaaagt
     1321 tgttgcccgt cggccggcga ttcttcagct ccacgcagcc gatttcgatc acgcggtgct
     1381 tctggctcac ttccagcccg gtggtttccg tgtcgagcac gatctgcctg ctcatttttc
     1441 ccccgctcct gcggccagcg ccgccatctc gtctatgcct tcattggcca gtgcatcggc
     1501 cagctcatta cccggatcgc cggcgtggcc ctttacccac ttccactcga tgcgatgccg
     1561 cgccgccgcc gcttccagct gctgccacaa ctcctg
//
LOCUS       DASZZC010000548         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_48545,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000548 DASZZC010000000
VERSION     DASZZC010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48545"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VHE37_11660"
     CDS             complement(<1..1039)
                     /locus_tag="VHE37_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36237.1"
                     /translation="MSLPRALLSLFGWFAVIWAALIAATLPRWPLEARDAPVQLHVQG
                     DSSMATGIFLNTIEETGYELARRCPLAPKKRLVLLGGSGARAYRPQYFEHVTAADEVL
                     LLSMNFSNFSQLRQIADDLLACLDRSTAEQTAVVIIPTAYSFTTTQTRFDGPYTIYEV
                     EKRRNRLFAGPPGQVRPIVPRALAPLAVELWRPVLLGRYVVQRISARIHAQFKPAMTA
                     ERRDAHQQWEDEAMRAELPRLLPLPVAGEPYAPEQAGELRRLIEDLQQAHVHVVLAQQ
                     PTQQWLREFSPAYPATQQVLRDVAAEYRVPYIDQSGTGADAEFDGPLHALRTAEPLWA
                     ARLAEALKQLQH"
     gene            complement(1050..>1590)
                     /locus_tag="VHE37_11665"
     CDS             complement(1050..>1590)
                     /locus_tag="VHE37_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36238.1"
                     /translation="ALGRRFPSRRLAVAFSSVAFFVTFVLVGIWHGPNWPFMVCGLFL
                     GVGATVNQVTREIRARRGEPADTLVNRAIAQFWAGMTFCYIAVAITPFWLDPHEYAAL
                     LRSVLQPAALWVLPALFAVVGCVALLARLLSRAIDLTVARAGALAQSAPATALRFAVV
                     LLYVLATTSALPDFVYKGI"
BASE COUNT          254 a          548 c          576 g          212 t
ORIGIN      
        1 cgtgctgcag ctgcttgagc gcctcggcca gccgcgccgc ccacagcggc tcggcggtgc
       61 gcagcgcgtg caacgggccg tcgaactcgg catcggcgcc ggtcccggac tggtcgatgt
      121 aaggcacccg gtattcggcc gccacgtcgc gcagcacctg ctgcgtggcc ggatacgccg
      181 gcgagaactc gcgcagccat tgctgggtcg gctgctgcgc gagcaccaca tgcacgtggg
      241 cctgctgcag gtcttcgatc aaccgccgca gctcgccggc ctgttccggc gcgtacggct
      301 cgcccgccac cggcagcggc agcaggcgcg gcagctcggc gcgcatggct tcgtcttccc
      361 actgctggtg cgcatcgcgc cgctccgcgg tcatcgccgg cttgaactgc gcatggatgc
      421 gcgcggagat gcgctgcacc acgtagcgtc ccagcagtac cggccgccac agctccacgg
      481 ccagcggcgc cagtgcccgc ggcacgatcg gccgtacttg accgggcgga ccggcgaaca
      541 ggcggttgcg ccgcttctcc acctcgtaaa tggtgtacgg gccgtcgaag cgcgtctgcg
      601 tggtggtgaa cgaataagcc gtcgggatga ttactaccgc ggtctgctcg gccgtgctgc
      661 ggtcgaggca ggcgagcagg tcgtcggcga tctgccgcag ctgcgagaag ttggagaagt
      721 tcatcgacag cagcagcact tcgtcggcgg cggtgacgtg ctcgaaatac tgcggacggt
      781 aggcgcgcgc gccggaaccg cccagcagca ccagccgctt cttgggtgcc agcgggcagc
      841 gccgcgccag ctcgtagccg gtctcctcga tggtgttgag gaagatgccc gtggccatgc
      901 tgctgtcgcc ctgcacgtgc agctggactg gtgcgtcgcg cgcttccagc ggccagcgcg
      961 gcagcgtggc ggcgatcagc gcggcccaga tcacggcgaa ccagccgaac agcgagagca
     1021 gggcgcgcgg caggctcatc tagatgcccc tagatccctt tgtagacgaa gtcgggcagc
     1081 gcgctggtgg tggccagcac gtaaagcagc accacggcga agcgcagcgc ggtggccggt
     1141 gccgattgcg ccagcgcgcc ggcgcgcgcc accgtgaggt cgatggcgcg cgacagcagc
     1201 cgcgccagca atgcgacgca gccgacgacg gcgaacagcg cgggcagcac ccacagcgcc
     1261 gccggctgca gcaccgagcg cagcagcgcg gcgtactcgt gcgggtcgag ccagaacggc
     1321 gtgatcgcca ccgcgatgta gcagaacgtc atgccggccc agaactgcgc gatggcgcgg
     1381 ttgaccaggg tgtcggccgg ctcgccgcgg cgcgcgcgga tttcgcgcgt gacctggttg
     1441 acggtggcgc ccacgcccag gaacaggccg cacaccatga acggccagtt cggcccgtgc
     1501 cagatgccga ccagcacgaa ggtgacgaag aaggcgacgc tggaaaacgc cacggcgagc
     1561 ctgcggctgg ggaagcggcg gcccagcgcg
//
LOCUS       DASZZC010000549         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_684032,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000549 DASZZC010000000
VERSION     DASZZC010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684032"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1585)
                     /locus_tag="VHE37_11670"
     CDS             complement(92..1585)
                     /locus_tag="VHE37_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HVT36239.1"
                     /translation="MKRLVIAAIAAMLCACGGAATSPAVSAPAARQLPLLHAVRGTAP
                     GIYDDSNRQVILRGVNYNALGDYYQDNPLYPPVILPRASDFPRMAGIGLNSVRLIVHW
                     SRIEPQPGIYDTAYLALIQQQIEQAAASGLYVIVDMHQDAWGKYVASREAGDSCLPPL
                     AAAIGWDGAPQWATLTDGLTTCRVQQREIAPAVQAAFENFYLDTQGVQTQFVSAWQVL
                     ASAVARYPNVAGYDLFNEPSPGFAIGANNLTLLSALYGKLVTTIRAAESAQHAPAHIV
                     FFEPPGEWSIAGFTVTPPPTFTADDNIVFAPHLYCGESGQTPTADCFRFAALTASLYQ
                     STFWVGEWGYFGDPSTLADPDAAFAAQEDASLVGSSLWQWQQACGDPHTIGTPGGTPA
                     ATVTQLLLVGCPGDVDLGFVDAHRLTLSRTYPRYAPGALTQISSDIASGAATIAGNGS
                     GTLELWVPARIGSPQVSGGDAAVGSVDGGFIVRVPVSGSYSVQLTPR"
     gene            complement(1585..2754)
                     /locus_tag="VHE37_11675"
     CDS             complement(1585..2754)
                     /locus_tag="VHE37_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVT36240.1"
                     /translation="MTRGAALLLALACALPARAADDASAVFQGVSPSVYSIITQSASA
                     GMMGSGVVVRPGYIVTNFHVVKGATHIKVQHNGSEEEAALAASDAEHDLALLHVYALA
                     APAVRFGDSQGLRVGQAVYAIGSPRGLELSLSAGLVSSLRVAKDGNVIQTTAPISPGS
                     SGGGLFDQYGRLVGITVGQISNAQNLNFALPVEWLHYVGVAVKASDVSELQNGAAVTT
                     AMATAEPPVPQNLAQPLNETPPSAPATSPPPHAQTPAAAPAKAPAPAAGKPPATNLYV
                     VTGTIVLLLVLAIPAARRLADFMSSDTLPDPPRNARAAPTRAQRLEPFRQQARDELKS
                     ERRDDDTWLRALEQAHGDQPRALVAYIDLRSEALYKAEMDRRWNSARSNQPGPRA"
     gene            complement(2751..>2995)
                     /locus_tag="VHE37_11680"
     CDS             complement(2751..>2995)
                     /locus_tag="VHE37_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HVT36241.1"
                     /translation="KGLPFRDAHHAVGAAVGYAAKKNCDLSDLPLRELQQFSPLIEKD
                     VYARITLEGSLAARNHYGATSPKQVRAAIARARGRA"
BASE COUNT          451 a         1010 c         1072 g          462 t
ORIGIN      
        1 gtgagttgca gaagaaatcg gaaaagctca ccgccgaaat cctcgagggc ctgggcaaga
       61 agggccccgg cggcaacaac aagaagctgc actagcgcgg cgtcagttgc accgagtagc
      121 tgccactcac cggcacgcgc acgataaaac caccgtccac cgacccgacc gccgcgtcgc
      181 cgccgctgac ctgcggcgag ccgatgcgcg cgggcaccca gagttccagc gtgccgctgc
      241 cgttgccggc gatcgtggcc gcgccgctgg cgatatcgga gctgatctgt gtgagcgcgc
      301 ccggcgcgta gcgtggatag gtgcgcgaca gcgtcagccg gtgggcatcg acaaagccga
      361 gatcgacatc accggggcag ccgaccagca gcagctgcgt gaccgtcgcc gccggtgtgc
      421 cgcccggcgt gccgatggtg tgcggatcgc cgcaggcctg ttgccattgc cacagcgagc
      481 tgccgaccag cgaagcgtct tcctgcgcgg cgaaagcggc gtcggggtcg gccagcgtcg
      541 acggatcgcc gaagtaaccc cattcgccca cccagaacgt gctctggtac agcgacgccg
      601 tgagcgcagc gaagcggaag caatcggcgg tcggcgtctg gccggattcg ccgcaataca
      661 ggtgcggcgc gaacacgatg ttgtcatcgg ccgtgaacgt gggcggcggc gtcaccgtga
      721 agccggcgat ggaccattcg cccggcggct cgaagaacac gatatgcgcc ggcgcgtgct
      781 gcgcgctttc cgccgcgcgg atggtcgtca ccagcttgcc gtagagcgct gacagcagcg
      841 tgaggttgtt ggcgccgatg gcgaagcccg ggctgggttc gttgaacagg tcatagccgg
      901 ccacgttcgg atagcgcgcg acggccgacg ccagcacctg ccaggccgag acgaactgcg
      961 tctgcacgcc ctgcgtgtcg aggtagaaat tctcgaacgc cgcctgcacc gccggcgcga
     1021 tctcgcgctg ctgcacgcgg caggtggtga ggccgtcggt gagcgtggcc cattgcggcg
     1081 cgccgtccca gccgatggcc gctgcgagcg gcggcaggca gctgtcgccg gcttcgcgcg
     1141 aggccacgta cttgccccag gcgtcctggt gcatgtcgac gatcacgtac agcccgctgg
     1201 ccgccgcctg ctcgatctgc tgctggatca gcgcgaggta ggcggtgtcg tagatgcccg
     1261 gctgcggttc gatgcgcgac cagtgcacga tcagccgcac gctgttcagg ccgatgccgg
     1321 ccatgcgcgg gaaatcgctg gcgcgcggca ggatcaccgg cgggtagagc gggttgtcct
     1381 ggtagtaatc gcccagcgcg ttgtagttca cgccgcgcag gatcacctgg cgattgctgt
     1441 cgtcgtagat gccgggcgcc gtgccgcgca cggcatgcag caacggcagc tgccgtgctg
     1501 ccggtgcgga aacggcaggc gaagtggccg cgccgccgca agcgcaaagc attgccgcga
     1561 tcgctgcgat gaccaggcgt ttcatcaggc gcgtggtccc ggttgattgc tgcgcgcgga
     1621 gttccagcgg cggtccatct ccgccttgta cagcgcttcg ctgcgcaggt cgatgtaggc
     1681 caccagcgcg cgcggctggt cgccatgcgc ctgttccagc gcgcgcagcc aggtgtcgtc
     1741 gtcgcgacgc tcactcttca actcgtcgcg tgcctgctgg cggaacggtt ccaggcgctg
     1801 cgcgcgtgtg ggcgcggcgc gtgcgtttcg gggcggatcg ggcagcgtgt cgctggacat
     1861 gaagtcggcc agccgcctgg ccgcggggat ggcgagcacc agcagcagga cgatcgtgcc
     1921 ggtgacgacg tagaggttgg tggctggcgg cttgcctgcc gccggcgcgg gcgcctttgc
     1981 cggtgcggca gcaggcgttt gcgcatgcgg cgggggcgac gtcgccggcg ctgaaggtgg
     2041 tgtttcgttg agcggctgcg ccaggttctg cggcaccggc ggctcggcgg ttgccatcgc
     2101 cgtggtcacc gccgcaccgt tctgcagttc ggaaacgtcg ctggccttca ccgccacgcc
     2161 gacgtagtgc agccattcca ccggcagcgc gaagttgagg ttctgcgcgt tgctgatctg
     2221 gccgacggtg atgccgacca gccgcccgta ctgatcgaac agcccgccgc cggacgagcc
     2281 cggcgagatc ggcgccgtgg tctggatgac attgccgtcc ttggccacgc gcagcgacga
     2341 caccagcccg gcggacagcg acagctccag cccgcgcggc gagccgatgg cgtacaccgc
     2401 ctgcccgacg cgcaggccct ggctgtcgcc gaagcgcacc gccggcgcgg ccagcgcgta
     2461 gacatgcagc agcgcaagat cgtgctcggc atcgctcgcg gccagcgcgg cttcctcttc
     2521 gctgccgttg tgctgcacct tgatgtgggt ggcgcctttc acgacgtgaa agttggtgac
     2581 gatgtagccg ggccgcacca caacgccgct gcccatcatg ccggcgctgg ccgactgcgt
     2641 gatgatcgaa tagacgctgg gcgacacgcc ctggaacacg gccgaggcat cgtccgccgc
     2701 acgcgccggc aatgcgcagg ccagcgccag caacagagcc gccccgcgtg tcacgcgcgg
     2761 ccccgcgccc gcgcgatggc ggcgcgcacc tgtttcggcg aggtggcgcc gtagtggttg
     2821 cgcgcggcca gcgagccttc cagcgtgatg cgtgcgtaca cgtccttctc gatcagcggc
     2881 gagaactgct gcagttcgcg cagcggcagg tcggacaggt cgcaattctt tttcgccgcg
     2941 taacccaccg cggcgccgac cgcatggtgc gcgtcgcgga acggcaggcc cttgc
//
LOCUS       DASZZC010000550         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1260803,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000550 DASZZC010000000
VERSION     DASZZC010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260803"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VHE37_11685"
     CDS             complement(<1..684)
                     /locus_tag="VHE37_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36242.1"
                     /translation="MPALAELARGTVLWLDAHEYAARLLANDVPPWLDVAAYVAYARK
                     AHGLLKPDVATLPLAPAIDAWLDAHAELRAQMAAKSRATYPLRVLLADEALRAHLLEL
                     ARGLRAALSGPLALVAPTPCAWISRAYVQAHHASVEVGDDEIDSAAVYLADFLRGFGE
                     CGVDALLLEESEGCLGGNFSAYQSVFNVAAHYRWDAGLRLPAAGDAHLPDGCSYAIAP
                     RPINGTLTAI"
     gene            complement(688..1464)
                     /locus_tag="VHE37_11690"
     CDS             complement(688..1464)
                     /locus_tag="VHE37_11690"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVT36243.1"
                     /translation="MTGTRVKDKVCIVTGGARGIGLATAERLLQEGAKVLITDVDEAA
                     GTAEQARLAKLGYPVAFHAHDVSWSAHWTAVLDAVFARWARLDVLVNNAGVAALGTIE
                     TQTVTDWRRTLGANLDSVFLGMQQAIPRIKGARGSIINIASIEGMVGEPQLPAYNSSK
                     GAVRILTRSAAIHCARSGYNIRVNSICPGFAETAMVANGLASLGAEGAQQFAAATLQR
                     IPMGRFAQPVEIANCVLFLASDEASYITGSDLVVDGGFTA"
     gene            complement(1477..>2002)
                     /locus_tag="VHE37_11695"
     CDS             complement(1477..>2002)
                     /locus_tag="VHE37_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36244.1"
                     /translation="VLVNNAGIETAALLAQCTLDDFSRVMNVNVNGVFLGLKHAVRVM
                     KPGSSIVNLSSVAGLIGTTGHIAYHTSKGAVRLMSKAAAVECAQLGTGIRVNSVHPAI
                     VQTEMGTNFIQHAVDLGLAPDYATAEAAFKAGHLLGMGMPSDVASGVLYLASDAARWI
                     TGAELVLDGGYTAV"
BASE COUNT          300 a          695 c          699 g          308 t
ORIGIN      
        1 gatcgcggtg agcgtgccgt tgatcgggcg cggcgcgatg gcgtagctgc acccgtcagg
       61 caagtgcgca tcgcccgccg cgggcaggcg caggccggcg tcccagcggt aatgcgccgc
      121 gacgttgaac acgctctggt aggcggagaa attgccgccc aggcagccct cactttcctc
      181 cagcagcagc gcgtcgacgc cgcactcgcc gaagccgcgc aaaaaatccg cgaggtagac
      241 cgcggcgctg tcgatctcgt cgtcgcccac ctcgacactc gcgtggtgcg cctgcacata
      301 ggcgcggctg atccacgcgc agggcgtggg cgccaccagt gcgagcgggc cgctgagcgc
      361 cgcgcgcagt ccgcgcgcca gctcgagcag gtgcgcgcgc agtgcctcgt ccgccagcag
      421 cacgcgcagc ggataggtgg cgcgcgattt ggccgccatc tgcgcgcgca gttctgcgtg
      481 ggcatcgagc caggcgtcga tggctggcgc cagcggcagc gtcgccacat cgggtttgag
      541 caacccgtgc gccttgcgcg cgtaggccac gtacgccgcg acgtccagcc acggcggcac
      601 atcgttggcc agcaagcgcg cggcgtactc gtgcgcgtcg agccacaaga ccgtgccgcg
      661 tgcgagttcg gcgagcgcgg gcatgggcta cgcagtgaag ccgccgtcga ccaccaggtc
      721 ggagccggtg atgtagctgg cttcgtcgga agccaggaac agcacgcagt tggcgatctc
      781 caccggctgc gcgaagcggc ccatggggat gcgctgcaag gtcgccgccg cgaactgctg
      841 cgcgccttcc gcgccgaggc tggcgaggcc attggccacc atcgcggttt cggcaaagcc
      901 cgggcagatg ctgttgacgc ggatgttgta gccgctgcgc gcgcagtgga tcgcggccga
      961 gcgcgtgagg atgcgcaccg cgcccttgct gctgttgtag gccggcaact gcggctcgcc
     1021 gaccatgccc tcgatgctgg cgatgttgat gatcgacccg cgcgcgccct taatgcgcgg
     1081 gatcgcctgc tgcatgccga ggaacacgct gtcgaggttg gcgcccagcg tgcgccgcca
     1141 gtcggtgacg gtctgcgtct cgatggtgcc cagcgcggcg acgccggcgt tgttcaccag
     1201 cacgtcgagc cgggcccacc gggcgaacac cgcgtcgagc accgccgtcc agtgcgccga
     1261 ccaactgacg tcatgcgcgt ggaacgccac ggggtagccg agcttggcca gccgcgcctg
     1321 ttcggcggta ccggcggctt catcgacatc ggtgatgagc accttggcgc cttcctgcag
     1381 caggcgctcg gctgtcgcca gcccgatgcc gcgcgcgccg ccggtgacga tgcagacctt
     1441 gtccttcacg cgggtgccgg tcatgtgcgc gctccgctat accgcggtgt aaccgccgtc
     1501 gagcaccagc tccgcgccgg tgatccagcg cgcagcatcg gaggcgaggt acagcacacc
     1561 cgaggcgaca tccgacggca tgcccatgcc gagcagatgc ccggccttga atgccgcttc
     1621 ggccgtggcg taatccggtg ccagccccag gtcgaccgcg tgctggatga agttggtgcc
     1681 catctcggtc tgtacgatgg ccgggtgcac cgagttcacg cggatgccgg tgcccagctg
     1741 cgcgcattcc accgccgccg ccttgctcat caggcgcacc gcgcccttcg acgtgtggta
     1801 cgcgatgtgg ccggtggtgc cgatcagccc ggccaccgag gaaaggttga cgatcgagct
     1861 gccgggcttc atcacgcgca ccgcgtgctt gaggccgagg aacacgccgt tgacgttgac
     1921 gttcatcacc cggctgaaat catccagcgt gcattgcgcg agcagcgccg cggtctcgat
     1981 gccggcattg ttgaccagca cg
//
LOCUS       DASZZC010000551         9421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1139727,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000551 DASZZC010000000
VERSION     DASZZC010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139727"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VHE37_11700"
     CDS             <1..604
                     /locus_tag="VHE37_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT36245.1"
                     /translation="NCGFALCSFASYGASAWIPTFMQRTYGWNIQQIGLAYGLVIMIF
                     GSAGIVVGGRLSDRMMRKGISEAPLRVGLWGALGTLPFGALYPLMPTGLGSVLMMAPT
                     VFFLAMPFGVAPAAIADIVPPAMRGQASAVYLFIVNLIGLGLGPTAVALFTDQVLHDP
                     QAIRWSLMWVGGAALAGAVALLSLGLAPYRASWSRLHATS"
     gene            complement(878..2188)
                     /gene="aceA"
                     /locus_tag="VHE37_11705"
     CDS             complement(878..2188)
                     /gene="aceA"
                     /locus_tag="VHE37_11705"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HVT36246.1"
                     /translation="MTTREEQIKALEKDWATNPRWKLVKRGYSAADVVRLRGSLQVEH
                     TLAKRGAEKLWKLVNGEAKKGYVNAFGAITAGQAMQQAKAGLEAVYLSGWQVAADGNT
                     SETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWSRGIEPGNKDFVDYFLPIVADAE
                     AGFGGVLNAFELMKNMITAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEACEKLIAAR
                     FAADVMGVPTIVLARTDAEASNLLTSDHDKNDKPFLTGERTQEGFYRVKNGLEQAISR
                     GVAYAPYADLVWCETGVPDIGFAREFAQAVHKACPGKLLSYNCSPSFNWKKNLNDAQI
                     AKFQDDLSALGYKYQFITLAGIHINWFNTFQFAHAYARGEGMKHYTEMVQEPEFKARD
                     KGYTFVSHQQEVGAGYFDDVTTVIQGGSSSVKALKGSTEEEQFH"
     gene            complement(2270..3850)
                     /gene="aceB"
                     /locus_tag="VHE37_11710"
     CDS             complement(2270..3850)
                     /gene="aceB"
                     /locus_tag="VHE37_11710"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HVT36247.1"
                     /translation="MSGVKGVVIAAPVSAEHKKILTPAALEFVAKLVRKHGPRREQLL
                     ALRKERQAAIDAGKLPGFLKETKKVRDGKWKIAGIPADLLDRRVEITGPVDRKMIINA
                     LNSGAKCFMADFEDSASPTWQLMMDGQINLRDAVARKIDYTSPEGKEYKLNQQIATLI
                     VRPRGWHLLEKHILVDGKPAPGGIVDFALYFFHNAKTLLKKGTGPYFYLPKMQSHLEA
                     RLWNAIFNDAQDALKIPRKTIKATVLIETLWAAFEMEEILYELRDHIVALNCGRWDYI
                     FSCIKTLNAHGDWMVPDRLQVTMDKHFLDSYSKLLCETCHKRGAMAMGGMAAFIPTKD
                     PVKNQEVFAKVAADKLREVKNGHDGTWVAHPGLVPVAMDVFNTHMPEANQLAKQFKYG
                     IKAADLLRKPEGQMTEAGLRNNINVGVQYTEAWLRGNGCVPLHNLMEDAATAEISRTQ
                     VWQWQKYGVTLSDGRKVTPALIRQILDEELDGIRAAMGDEKYFATRMTEAAGIMARMS
                     TAAKCTDFLTLPSYDYLD"
     gene            complement(3981..5345)
                     /locus_tag="VHE37_11715"
     CDS             complement(3981..5345)
                     /locus_tag="VHE37_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVT36248.1"
                     /translation="MKDMTTGPVRGHVLRMAAFMLVSMLVQTAYSLIDMFWVARLGAQ
                     AAAAVGVASNLMFVVLALSQALGVGTVALVSQSYGRKDEASVQRWFNQSLALSVLSGL
                     LFAAIGFGMMRAYTDFMSGDAVTAELARRFLAGFIPAMALQFSMTGLGSSLRAIGTMK
                     PGLYAQTGSVLLNMALAPVLIFGWLGAPALGVLGGAMASLIAALASIAGLALYLRRGS
                     TYLRVNFTQWKPDLHGWKHMLLIGLPSGLEMVVTAIYVSLIFWVIRPFGADAQAGFGI
                     GMRLMQTGFMPAMALSFGAAAVVGQNVGARNFQRVKHTFYVSARMCGIFMLGFTVLCQ
                     LSSGAMMQLMSHDAPVIAVGADYLKYISWNYLANGLGFIAGGVFQGMGYTLPSLLASA
                     ARMSTFVASVLWLSHRPGFTLHQVWLTSIATVTLQVAIVLVLLRSELKRRLAGQTPLP
                     ATPA"
     gene            complement(5379..5864)
                     /gene="rraA"
                     /locus_tag="VHE37_11720"
     CDS             complement(5379..5864)
                     /gene="rraA"
                     /locus_tag="VHE37_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HVT36249.1"
                     /translation="MSLATTDLSDAHADLQVCEPLFTDFGGVLAFHGQIKTLKIFEDN
                     ALVRAALETPGNGRVLVVDGGGSMRCALVGGNLGALAVKNGWSGIVVNGCIRDSEEIA
                     GQEVGVKALATHPRKSEKGLHSGHEDKAVTFAGVTFRSGEYLYADGDGIVVSARALHS
                     A"
     gene            5968..6945
                     /locus_tag="VHE37_11725"
     CDS             5968..6945
                     /locus_tag="VHE37_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HVT36250.1"
                     /translation="MYSLTARITRTGGPEVIELARQDLLAPGAGEVLLRHTAIGVNFI
                     DTYHRSGLYAVPLPSGLGTEAAGVVEAVGSGVTNLRAGDRVAYAGGALGAYAQLRVMP
                     ANKLVRVPDAVSDEAAAALMLKGMTAYYLLHLTYAVKRGETALIHAAAGGVGSLLVPW
                     AKALGVRVIGTAGGSAKCALARQYGCDEVIDYSSADFVTAVKRLTDGRGVDVVYDSVG
                     KTTLPGSLDCLRRRGMAVTFGNASGKPAAVEPNTLTQKGSLYLTRPTLADYTATREEL
                     ETAANVVFDALARGIIRADVRQRYPLADAARAHADLEARKTTGATILLP"
     gene            complement(7044..7553)
                     /locus_tag="VHE37_11730"
     CDS             complement(7044..7553)
                     /locus_tag="VHE37_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HVT36251.1"
                     /translation="MKINFRLLCVGGFLFTVASMLFALYLQHYKGFEPCPMCIFQRIA
                     MMTSGFFFLLGAIHGPRAGGRWIYAGLADLAAIVGALIAARHVWLQSLPEDLRPACGP
                     TLDYLLDMMPMKDAVEFVLRGEGQCAKIDAQWLGLSLPFWTMIGFIVLSFYAALTPVV
                     AKLFERRVA"
     gene            complement(7698..9221)
                     /locus_tag="VHE37_11735"
     CDS             complement(7698..9221)
                     /locus_tag="VHE37_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HVT36252.1"
                     /translation="MTAIRQQDLIDSVADAFQYISYYHPLDYIQALGAAYEREESPAA
                     RDAIAQILTNSRMCAEGHRPICQDTGIAVVFIKVGMQVSWPDATMSVQEMINEGVRRA
                     YNHPDNKLRASVLLDPAGKRTNSKDNTPAVVHFEIVPGHHVEVICAAKGGGSENKSKM
                     VMLNPSDSIVDWVLKTLPAMGAGWCPPGILGIGIGGTPEKAMLLAKESLMAPVDIQEL
                     RARGPKNRIEELRLELMDKVNALGIGAQGLGGLTTVLDVKIMDYPTHAASLPVAMIPN
                     CAATRHVHFHLDGSGPAHLPTPKLEDWPQVTWKADLKTATRVNLDTLTKEEVASWKPG
                     QTLLLNGKMLTGRDAAHKRIADMLGKGEKLPVDFTNRAIYYVGPVDPVRDEAVGPAGP
                     TTATRMDKFTEMMLAQTGLIVMIGKSERGPAGIAAIRKHKAAYLMAVGGAAYLVSKAI
                     KSAKVLGFADLGMEAIYEFEVQDMPVTVAVDAAGTSVHETGPKVWQAKIGKIPVAVA"
BASE COUNT         1557 a         3126 c         3008 g         1730 t
ORIGIN      
        1 caactgcggt ttcgcgctgt gctcgttcgc ctcctacggc gcatcggcct ggattcccac
       61 cttcatgcag cgcacctacg gctggaacat ccagcagatc ggcctggcct atggactggt
      121 catcatgatt ttcggcagcg ccgggatcgt ggttggcggc cggctgtccg atcgcatgat
      181 gcgcaagggc atcagcgaag cgccgctgcg cgtgggcctg tggggcgcgc tgggcacgct
      241 gccgttcggc gcgctgtatc cgctgatgcc caccggcctg ggctcggtgc tgatgatggc
      301 gcccaccgtg ttcttcctcg ccatgccctt cggcgtggcg cccgccgcca tcgccgacat
      361 cgtcccgccg gcgatgcgcg gacaggcgtc ggctgtgtac ctgtttatcg tcaacctgat
      421 cggcctgggc ctcggcccta ctgccgtcgc gttgttcacc gaccaggtgc tgcacgaccc
      481 gcaggccatc cgctggtcgc tgatgtgggt gggcggcgcc gcgctggccg gcgcggtcgc
      541 gctgctgtcg cttggccttg cgccgtatcg cgccagctgg tcgcgcctgc acgcaaccag
      601 ctgagaagcg aaaatccgcg ctgctgcagc tccaccgcaa tgccgtgtgg catgttataa
      661 ataattatct aaatttaata tcagataatg ttttgtaaca cgccacaacg gcttgcggtg
      721 gggcccaatc aaggctgtcg gccagcccat tcgcgctgcc tgatccgtga cacgacacgc
      781 agacgctgaa tatctctgcc gatcgcgctg acccacgact cactcgcgca aagaaaaagg
      841 gccgcgatag cggccctctt tcgctccacc ttgctgctta gtggaactgt tcttcttccg
      901 tcgaaccctt cagcgccttc acgctcgacg agccgccctg gatcacggtg gtcacatcgt
      961 cgaagtagcc cgcgcccact tcctgctggt ggctgacgaa ggtgtagccc ttgtcgcgcg
     1021 ccttgaactc cggctcctgc accatctcgg tgtagtgctt catgccttcg ccgcgcgcgt
     1081 aggcgtgcgc gaactggaag gtgttgaacc agttgatgtg gatgccggcc agcgtgatga
     1141 actggtactt gtagcccagc gccgacaggt cgtcctggaa cttggcgatc tgcgcgtcgt
     1201 tgaggttctt cttccagttg aacgacggcg agcagttgta gctgagcagc ttgcccgggc
     1261 aggccttgtg cacggcctgc gcgaattcac gcgcgaagcc gatgtccggc acgccggtct
     1321 cgcaccacac caggtcggcg tacggcgcgt aggccacgcc gcggctgatg gcctgctcga
     1381 ggccgttctt cacgcggtag aagccttcct gcgtgcgctc acccgtcagg aacggcttgt
     1441 cgttcttgtc gtggtcgctg gtcagcaggt tggaagcttc cgcgtcggtg cgcgccagca
     1501 cgatggtcgg cacgcccatg acgtcggcgg cgaagcgcgc ggcgatcagc ttctcgcagg
     1561 cttcctgcgt cggcaccagc accttgccgc ccatgtggcc gcacttcttc accgcggcca
     1621 gctggtcttc gaagtgcacg ccggcagcgc cggcggtgat catgttcttc atcagctcga
     1681 aggcgttgag cacgccaccg aaaccggcct cggcgtcggc cacgatcggc aggaagtaat
     1741 cgacgaagtc cttgttgccc ggctcgatgc cgcgcgacca ctggatctcg tcggcgcgct
     1801 tgaaggtgtt gttgatgcgg cggaccatgg tcggcaccga gtcgtacgcg tagagcgact
     1861 ggtcggggta catggtttcg ctggtgttgc cgtcggcggc aacctgccag cccgacaggt
     1921 acacggcttc aaggccagcc ttggcctgct gcatcgcctg gccggcggtg atcgcgccga
     1981 aggcattcac ataacccttc ttggcttcgc cgttcaccag cttccagagc ttctcggcgc
     2041 cgcgcttggc gagcgtgtgc tcaacctgca ggctgccgcg caggcgcacg acgtcagcgg
     2101 cggagtaacc gcgcttgacc agcttccagc gtgggttggt ggcccagtcc ttctcaaggg
     2161 ccttgatctg ctcttcgcgc gtcgtcattg cagctttcct ctaggggtga aaaaagaaat
     2221 tcgtaaaaat tcgtaaatgg ggatgccaat accctggaac ccgcaacgct caatccaggt
     2281 agtcgtagct cggcagcgtc aggaagtcag tgcacttcgc cgcggtcgac atgcgcgcca
     2341 tgatgccggc ggcctcggtc atgcgcgtgg cgaaatactt ctcgtcgccc atcgccgcac
     2401 ggatgccgtc gagttcctcg tcgaggatct ggcggatcag cgccggcgtc accttgcggc
     2461 catcgctcag cgtcacgccg tatttctgcc actgccacac ctgcgtgcgg ctgatctcgg
     2521 cagtcgccgc atcttccatc aggttgtgca gcggcacgca gccgttgccg cgcagccagg
     2581 cctcggtgta ctgcacgccg acattgatgt tgttgcgcag gccggcctcg gtcatctgcc
     2641 cttcgggctt gcgcagcagg tcagcggcct tgatgccgta cttgaactgc ttggccagct
     2701 ggtttgcttc cggcatgtgc gtgttgaaca cgtccatcgc caccggcacc agccccgggt
     2761 gcgccaccca ggtgccgtcg tggccgttct tcacctcgcg cagcttgtcg gcggcgacct
     2821 tggcgaacac ttcctggttc ttgaccggat ccttggtcgg gatgaaggcg gccatgccac
     2881 ccatcgccat ggcgccgcgc ttgtggcagg tctcgcacag cagcttgctg tagctgtcga
     2941 ggaaatgctt gtccatggtg acctgcaggc gatcgggcac catccagtcg ccatgcgcgt
     3001 tgagcgtctt gatgcagctg aagatgtaat cccagcggcc gcagttgagc gccacgatgt
     3061 gatcgcgcag ctcgtacagg atttcttcca tctcgaacgc ggcccacagc gtttcgatca
     3121 gcacggtggc cttgatggtc ttgcgcggaa tcttcagcgc gtcctgcgca tcgttgaaga
     3181 tggcgttcca cagccgcgcc tcgaggtgcg actgcatctt cggaagatag aagtacgggc
     3241 cggtgccctt cttcagcagc gtctttgcgt tgtggaagaa atacagcgcg aagtcgacga
     3301 tgccgccggg cgccggcttg ccgtcgacca ggatgtgctt ttccagcagg tgccagccgc
     3361 gcggacgcac gatgagcgtg gcgatctgct ggttgagctt gtattccttg ccctcgggcg
     3421 aggtgtagtc gatcttgcgg gcgaccgcat cgcgcaggtt gatctggccg tccatcatca
     3481 gctgccaggt cggcgaggcg ctgtcctcga agtccgccat gaagcacttc gcgccgctgt
     3541 tgagcgcgtt gatgatcatc ttgcggtcga ccggcccggt gatctccacg cggcgatcca
     3601 gcaggtcggc gggaatgccg gcgatcttcc acttgccgtc gcggaccttc ttggtctctt
     3661 tcaggaaacc ggggagcttg cccgcatcga tggcggcctg gcgctccttg cgcagcgcga
     3721 gcagctgctc gcggcgcggg ccatgcttgc ggaccagttt tgcgacgaat tccagcgccg
     3781 cgggagtgag gattttcttg tgctcggccg aaacgggagc agcaatcacc acgcctttaa
     3841 caccactcat ccgaaaactc ctgcctgttc ttgggatgga agcgcgagcc gggcggggct
     3901 cgcgagggcg cgcattatag gcgaaagtga agcgtgcgtt gcgatgcagc aaaagccctg
     3961 tttcaacgga ggagatctgc tcaggcaggc gtcgcaggca gcggcgtttg ccccgccagc
     4021 cggcgcttca attcgctgcg cagcagcacc agcacgatgg ccacctgcag cgtcaccgtg
     4081 gcgatggagg tcagccacac ctggtgcagc gtgaagcccg gccggtgcga cagccacagc
     4141 accgacgcca cgaaggtgct catgcgcgcc gccgaggcca gcagcgacgg cagcgtgtaa
     4201 cccatgccct ggaacacgcc gccggcgatg aagcccagcc cgttggccag gtaattccac
     4261 gagatgtatt tcaggtaatc cgcgcccacc gcgatcacgg gcgcatcgtg cgacatcagt
     4321 tgcatcatcg cgcccgacga caactggcac agcacggtga agcccagcat gaagatgccg
     4381 cacatgcgcg cgctgacgta gaaggtgtgc ttcacgcgct ggaaattgcg cgcgcccacg
     4441 ttctgcccca ccaccgcagc ggcgccgaac gacagcgcca ttgccggcat gaagccggtc
     4501 tgcatcaggc gcatgccgat gccgaagccg gcctgcgcgt cggcgccgaa cgggcggatc
     4561 acccagaaga tcaggctgac gtagatcgcg gtcaccacca tctccaggcc cgacggcagg
     4621 ccgatcagca gcatgtgctt ccagccgtgc aggtcgggtt tccactgggt gaagttcacg
     4681 cgcaggtagg tcgagccgcg gcgcaggtac agcgccaggc cggcgatcga cgccagcgcc
     4741 gcgatcagcg aggccatggc gccgccgagc acgcccagcg ccggcgcgcc gagccagccg
     4801 aagatcagca ccggcgccag cgccatgttc agcagcaccg aaccggtttg cgcatacagg
     4861 cccggcttca tggtgccgat ggcacgcagc gaagagccga gcccggtcat tgagaactgc
     4921 agcgccatgg ccgggatgaa gccggcgagg aagcgacgcg ccaactccgc ggtgacggcg
     4981 tcgccggaca tgaagtcggt atacgcgcgc atcatgccga agccgatggc cgcgaacagc
     5041 aaacccgaca gcactgacag cgccagcgac tggttgaacc agcgctgcac cgaggcctcg
     5101 tccttgcgtc cgtaggactg cgacaccagc gccaccgtgc ccacgcccag cgcctgcgac
     5161 agcgccagca ccacgaacat caggttggag gccacgccga cggcggccgc tgcctgcgcg
     5221 cccaggcgtg ccacccagaa catgtcgatc aggctgtagg cggtctgcac cagcatgctg
     5281 accagcatga aggcggccat gcgcagcacg tggccgcgga ccggcccggt ggtcatgtct
     5341 ttcatgggaa cacggccgcg cggggcgcgg cgcagactct aggcgctatg cagcgccctg
     5401 gcggagacga cgatgccgtc gccgtcggca tagagatatt cgccgctgcg gaaggtcacg
     5461 ccggcgaacg tcaccgcctt gtcctcgtgc ccgctgtgca ggcccttctc gctcttgcgc
     5521 ggatgcgtgg ccagcgcctt cacgccgact tcctgcccgg caatttcttc cgaatcgcgg
     5581 atgcagccgt tgacgacgat tccgctccag ccgttcttca ccgccagcgc gccgagattg
     5641 ccgccgacca gcgcgcagcg catggagccg ccgccgtcga ccaccagcac gcggccgttg
     5701 cccggcgttt ccagcgcggc gcgcaccagc gcgttgtcct cgaagatctt cagcgtcttg
     5761 atctggccgt ggaacgccag cacgccgccg aagtcggtga acagaggctc gcacacctgc
     5821 aggtcggcgt gcgcatcgga caggtcggtg gtggcgaggc tcatggtgtt tccccggcag
     5881 agcttgcagg catggcaaaa ggggcggcag attagcggcc cgcgcgcagc cgcgcaacgc
     5941 ggttttctcc aggaaaaatt gaacgccatg tacagcctta ccgcacgcat cacccgcacc
     6001 ggcggaccgg aagtgatcga gctcgcgcgg caggacctgc tggcgcccgg cgcgggcgaa
     6061 gtgctgctgc gccacaccgc catcggcgtg aacttcatcg acacctatca ccgctccggc
     6121 ctgtacgcgg tgccgctgcc cagcgggctg ggcaccgagg cggcgggcgt ggtcgaggcc
     6181 gtcggcagcg gcgtcacgaa tctgcgtgcc ggtgaccgcg tggcgtatgc gggcggcgcg
     6241 ctcggcgcct acgcgcagtt gcgcgtgatg ccggccaaca agctggtgcg cgtgcccgat
     6301 gcagtcagcg acgaagccgc cgccgcgctg atgctcaagg gcatgaccgc gtactacctg
     6361 ctgcatctca cctacgcggt gaagcgcggc gagaccgcgc tgatccacgc cgcggccggc
     6421 ggcgtcggca gcctgctggt gccttgggcc aaggcgctgg gcgtgcgcgt gatcggcacc
     6481 gccggcggca gcgccaagtg cgcgctggct cgccaatacg gctgcgacga ggtgatcgac
     6541 tacagcagcg cggatttcgt caccgcggtg aagcggctca ctgacggccg cggtgtcgac
     6601 gtggtctacg actcggtggg caagaccacc ctgcccggct cgctcgactg cctgcgccgg
     6661 cgcggcatgg cggtgacctt cggcaacgcc agcggcaagc cggcggcagt ggagccgaac
     6721 acgctcacgc agaagggctc gctgtatctc acgcggccga cgctggccga ctacaccgcc
     6781 acgcgcgagg aactggagac agcagccaac gtcgtgttcg atgcgctggc gcgcggcatc
     6841 atccgggccg atgtgcgcca gcgttacccg ctggccgacg cggcgcgcgc gcatgcggac
     6901 ctggaggcgc gcaagaccac cggtgcgacc atcctgcttc cgtaattcct gtcattcccg
     6961 ccaaggcggg aatccggttt ttctgcggtc gggaagggaa atcaagactg ggtccccgct
     7021 tcgcggggat gccgcaacgg cgttcaggcc acccggcgct cgaacagctt cgccaccacc
     7081 ggcgtcaacg cggcgtagaa cgacagtacg atgaatccga tcatggtcca gaacggcagc
     7141 gacaggccca gccactgcgc gtcgatcttg gcgcactggc cttcgccgcg cagcacgaat
     7201 tccaccgcgt ccttcatcgg catcatgtcg agcaggtagt cgagcgtagg gccgcaggcc
     7261 gggcgcaggt cctcaggcag cgactgcagc cacacgtggc gcgcggcgat cagcgcgccg
     7321 acgatcgcag ccaggtcggc caggccggcg tagatccagc gtccgccagc gcgcgggccg
     7381 tggatggcgc cgagcaggaa gaagaatccc gaagtcatca tggcgatgcg ctggaagatg
     7441 cacatcgggc acggctcgaa gcccttgtag tgctgcaggt acagcgcgaa cagcatcgag
     7501 gcgacggtaa agaggaagcc gccgacgcac agcagacgga aattgatttt catggttctc
     7561 gcgcgatcct gaccgccgtt tttctcttca ccgcaccgca atttttagtg ttattattta
     7621 ttatctgttt taacttttag ataatattta ataacagaca acaccggcat gcggtgagct
     7681 cttttttgcc gcttcactca ggcaaccgcg accggaattt tcccgatctt cgcctgccag
     7741 accttcggac cggtctcgtg cacggaggtt cccgcggcat cgaccgctac cgtcaccggc
     7801 atgtcctgca cctcgaattc gtagatcgct tccatgccca ggtcggcgaa gcccagcacc
     7861 ttggcggact tgatcgcctt cgacaccagg tacgccgcgc cgcccaccgc catcaggtac
     7921 gcagccttgt gcttcctgat cgccgcaatg ccggccgggc cacgctcgct cttgccgatc
     7981 atcacgatca gcccggtctg cgccagcatc atctcggtga acttgtccat gcgcgtcgcc
     8041 gtggtcggac ccgcggggcc aaccgcctcg tcgcgcaccg ggtcgaccgg gccgacgtag
     8101 tagatcgcgc ggttggtgaa atccaccggc agcttttcgc ccttgcccag catgtcggcg
     8161 atgcgcttgt gcgcggcatc gcggccggtg agcatcttgc cgttgagcag cagcgtctgg
     8221 cccggcttcc acgatgcgac ttcttctttt gtcagcgtgt cgagatttac ccgcgtggcg
     8281 gttttcagat cggccttcca ggtcacctgc ggccagtctt ccagcttcgg cgtcggcagg
     8341 tgcgccgggc cgctgccgtc gagatggaaa tgcacatggc gcgtcgccgc gcagttcgga
     8401 atcatcgcca ccggcaggct cgccgcgtgc gtcgggtaat ccatgatctt cacgtcgagc
     8461 acggtggtga ggccgccaag gccctgcgca ccgatgccca gcgcattgac cttgtccatc
     8521 agttccaggc gcagttcttc gatcctgttc ttcggcccgc gcgcgcgcag ttcctggatg
     8581 tccaccggcg ccatcagaga ttccttggcc agcagcatgg ccttctccgg cgtgccgccg
     8641 atgccgatgc cgagtattcc gggcgggcac cagcccgcgc ccatcgccgg caaggttttc
     8701 agcacccagt cgacgatgga gtcggaagga ttcagcatga ccatcttgga cttgttttcg
     8761 ctgccgccgc ccttggctgc gcagatcact tcgacgtgat gaccgggcac gatctcgaaa
     8821 tgcaccaccg ccggcgtgtt gtccttggag ttggtgcgct tgccggcggg gtccagcagc
     8881 accgaggcgc gcagcttgtt gtccgggtgg ttgtaggcgc ggcgcacgcc ctcgttgatc
     8941 atctcctgca cgctcatggt cgcgtcgggc cagctcacct gcatgcccac cttgatgaac
     9001 accaccgcga tgccggtgtc ctggcagatc ggccggtgtc cttccgcgca catgcgcgag
     9061 ttggtcagga tctgcgcaat cgcatccctg gccgccggcg attcctcgcg ctcgtacgcc
     9121 gcgcccagcg cctggatgta gtcgagcgga tggtagtagc tgatgtactg gaaggcgtcg
     9181 gcgacgctgt cgatcaaatc ctgctggcgg atggcggtca tggcgcgaat cacgaaaaat
     9241 gaagggcgcg attatacgac tgcaccctcg tcaaccgtgc ctcgattggg cagactcgcg
     9301 cgctcacacc gatggaatgc gccatgttga agaagatcat caagatcatc ggctggatcg
     9361 ttgccgtgct cgtgctgctg atcgccggcc tggcgctgta tgcctatgtc gacgggcgcc
     9421 c
//
LOCUS       DASZZC010000552         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1260965,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000552 DASZZC010000000
VERSION     DASZZC010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260965"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="mutM"
                     /locus_tag="VHE37_11740"
     CDS             complement(<1..812)
                     /gene="mutM"
                     /locus_tag="VHE37_11740"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HVT36253.1"
                     /translation="MPELPEVETTRRGVHPHIVGRTIEKAIVRDARLRWPVPREFARR
                     VEQKKITGTARRGKYLLIELAGGARVIVHLGMTGRLSVRERGHPLRKHDHIDLELSGG
                     LTLRFGDPRRFGAVLFWPADEPTHKLLSGMGPEPLSDDFSGAHLFDVSRGRSAAVKNF
                     IMDGRVVVGVGNIYASEALFRARIKPQRAAGKVTRAQYELLAKKIRDVLDDAIKAGGT
                     TLRDYSGAHGEPGEFKQKLYVYDRAGRPCLRCKTPIRRLVLGQRSSFYCPSC"
     gene            complement(995..>1799)
                     /locus_tag="VHE37_11745"
     CDS             complement(995..>1799)
                     /locus_tag="VHE37_11745"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HVT36254.1"
                     /translation="ADAVNLITIDHPSAALIKLERKGSSWFLTAPVQAQADPYEVNGV
                     LQLATSETKQTLDAAQVKPAELGLDPATYDVTFNDQKVEIGNVEPLKYRRYVRGGGRI
                     ALIEDPPSAALDADYSDLVAKNLVADGAKVVGVELPGLTLTQDADGKTLTLKPDDPKA
                     TAAQKAQLLEGWQRARAMWNALEGADSKLDPKADTVTLKLADGREQKYIVAARDPQLA
                     LVRPDLKIRYTLSKEDVDKLFKLPEEKPAPSADAKKADAAPLSTPAASN"
BASE COUNT          249 a          598 c          614 g          338 t
ORIGIN      
        1 tggcaggacg ggcaatagaa actggagcgc tggcccagga ccaggcggcg gatcggcgtc
       61 ttgcagcgca ggcacggccg gccggcgcgg tcgtacacgt agagcttctg cttgaactcg
      121 cccggctcgc cgtgcgcgcc ggagtagtcg cgcagcgtgg tgccgccggc cttgatcgcg
      181 tcatcgagca cgtcgcggat tttcttcgcc agcagctcgt actgcgcgcg cgtcaccttg
      241 ccggccgcgc gctgcggctt gatgcgcgcg cggaacagcg cctcgctggc gtagatgttg
      301 ccgacgccga ccaccacgcg accgtccatg atgaaattct tcaccgccgc gctgcgcccg
      361 cgcgaaacat cgaacaggtg cgcgccggag aaatcgtccg acagcggctc cggccccatg
      421 ccggagagca gcttgtgcgt gggctcgtcc gccggccaga acagcacggc gccgaagcgg
      481 cgcggatcgc cgaagcgcag cgtcagtccg ccggagagtt cgagatcgat gtgatcgtgc
      541 ttgcgcagcg ggtggccgcg ctcgcgcacc gacaggcgcc cggtcatgcc caggtgcacg
      601 atcacgcgcg cgccgccggc cagctcgatc agcaggtact tgccgcggcg cgcggtgccg
      661 gtgatttttt tctgctccac gcgtcgcgcg aactcgcgcg gcaccggcca gcgcaggcgc
      721 gcgtcgcgca cgatggcttt ctcgatggtg cggccgacga tatgcgggtg cacgccgcgg
      781 cgcgtggttt cgacttcggg aagttcgggc atgcgcggcg cgcggagaaa atgttggcgc
      841 aattctagct gcgcgcccgg cggtgcaagc gtgcgcacgc gtttttcagc tcaaccgcac
      901 tgccctgcag cgagttatta attatcgtcc aattttattt tcagacaatg attaataaca
      961 gcacaaaccc gggtgcggtg tcggcccgcg cgcctcagtt cgatgccgcc ggcgtgctca
     1021 gcggcgccgc gtcggctttc ttcgcgtccg cagacggcgc cggcttttcc tccggcagct
     1081 tgaacagctt gtcgacgtct tcctttgaca gcgtgtagcg gatcttcagg tcgggccgca
     1141 ccagcgccag ctgcggatcc cgcgcggcga cgatgtactt ctgctcgcgg ccgtcggcca
     1201 gtttcagcgt gacggtgtcg gcttttggat cgagcttgct gtccgcgccc tcgagcgcgt
     1261 tccacatggc gcgtgcgcgc tgccagcctt cgagcagctg cgccttctgc gcggccgtgg
     1321 ccttcgggtc atcgggcttg agcgtgagcg tcttgccgtc ggcgtcctgc gtcagcgtga
     1381 ggcccggcag ttccacgccg actacttttg caccgtcggc caccaggttc ttggcgacga
     1441 ggtcggagta atcggcatcg agcgccgcgc tgggcggatc ttcgatcagc gcgatgcgcc
     1501 cgccgccgcg cacgtagcgg cggtacttga gcggctcgac gttgccgatc tccactttct
     1561 ggtcgttgaa ggtcacgtcg taggtggcgg ggtcgaggcc gagctcggcc ggcttcacct
     1621 gcgcggcgtc gagggtttgc ttggtttccg acgtcgccag ctgcagcacg ccgttcactt
     1681 catacggatc ggcctgcgcc tgcaccggcg cggtgaggaa ccagctgctg cccttgcgct
     1741 ccagcttgat cagcgccgcg ctcgggtgat cgatggtgat gaggttcacc gcatcggcg
//
LOCUS       DASZZC010000553         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1139928,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000553 DASZZC010000000
VERSION     DASZZC010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139928"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1414
                     /locus_tag="VHE37_11750"
     CDS             41..1414
                     /locus_tag="VHE37_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT36255.1"
                     /translation="MKHGSGLVGLLLALAVNMPAFAQDEGSSDSGAAPASPIDGKPWY
                     VSPMFSYLLADKDRGTDDGYGGTITVGKRLTYGLNLELSGTYLQAKGKNQPGVIDNGT
                     AKLSDVAVTALIFPMTTHPNFFGILSVGMSGAKNHPGTIAEYRGTEFDAGLGYLFPIT
                     KKILLRAEARYRLDQSNRHDAGVQVPPKNNSEFYEGLFNVGLLIPLGSVAAPPAAPPP
                     EVATPPTNDSDGDGVPDDKDKCPGTPAGAIVDENGCEKDSDGDGVPDRLDKCPDTPAG
                     QKVGADGCPLDSDGDGIPDDVDECPHTPAGAKVLANGCALTGDCRTPRPGEQVDENGC
                     AVDKTFILKGVNFEFDSDRLTEPSKQILDQTAETLKSYPDIKVEVAGHTDNIGSDSYN
                     LGLSERRAIAVKNYLTGRGVDAARMSPNGYGLTQPIDSNDSEAGRAKNRRVELRVIEQ
                     QNANVGK"
     gene            1588..2541
                     /locus_tag="VHE37_11755"
     CDS             1588..2541
                     /locus_tag="VHE37_11755"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HVT36256.1"
                     /translation="MYKNRTITKHRTLWVSDVHLGSPGAKARELTDFLKRNDCEQLYL
                     VGDIFDGWKMKSRFYWTPDHSRVIKAIISKARRGTKVYYLAGNHDGFMRQFVKNQLRL
                     GKVRIAHEVVHTTADGRRLLVTHGDTFDDVINGLPWLAYAGDVAYEALMEASKLLNRA
                     GARMGLPYWSLSAFTKTAVKTAVQYWSGVDNKMLHRCRSENLAGVVSGHTHHAEIRML
                     RPGITLHNCGDWVESCTALTEDRDGNIEIVNYCPQPSNAGAAEAAIAAVSAPPPWVGA
                     VSRAAKNLGGIGRRAVRRSSRKPARVRTATAATDVAVAAGE"
     gene            2757..3200
                     /locus_tag="VHE37_11760"
     CDS             2757..3200
                     /locus_tag="VHE37_11760"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HVT36257.1"
                     /translation="MDIPLDKRPAGTFYTGGVIQGYGRLDMLVDTGSSILVIDENILS
                     QLVQAGEAEYSRQLGGLMADGTQRHVPVYRVHALRVGDNCWIRNVEAAVFPAGSRAIL
                     GMNVLTRLAPFTFSADPPQLQLNQCLATPAEAMGGTGGAPVAASR"
     gene            complement(3231..3647)
                     /locus_tag="VHE37_11765"
     CDS             complement(3231..3647)
                     /locus_tag="VHE37_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36258.1"
                     /translation="MRKLSFVIALASFAGAALAAGNTVTPGSNSSTNSTLQNSGSATA
                     GGANSGYSGSGYPGTTSTTGTAGASGSVQTSGSSGAFAKVDADGDGMITKKEAAKDKM
                     LGKKFDSLDLNHDGKLDTMEFSQFESTGTSTDTSRH"
     gene            complement(3753..>4742)
                     /locus_tag="VHE37_11770"
     CDS             complement(3753..>4742)
                     /locus_tag="VHE37_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HVT36259.1"
                     /translation="VHDYHLIPLGAELRKLGARQRLGFFLHTPFPPADILETLPGHQE
                     LMQALFQYDVVGLQTQPDVERLQGYVQRYAGGAVDGEQLRVQGRRLVLRAFPIGIDAA
                     QFQKVAMSAEGMREGGRMREALNGRALVIGVDRLDYSKGLLRRMAGYELLLERYPALH
                     RQVELLQIAPISRGELKAYKDFRRELEHAAAGINGRYAQADWTPVRYLNRALPRRALG
                     GLYRASRVGLVTPVRDGMNLVAKEYVAAQDPADPGVLVLSRFAGAAQQMHEALLVNPY
                     DEAAMAEALHQALVMPLAERRERHAALMRGLLAYDVRRWRDEFVASLSAAPAG"
BASE COUNT          829 a         1611 c         1556 g          746 t
ORIGIN      
        1 cgataccgat tatttcaaga accgtcagag gaacaaatat atgaaacacg gctcgggtct
       61 tgtggggttg ttgctggcgc tggcggtgaa catgcccgcc ttcgcccagg acgaggggag
      121 cagcgacagc ggcgccgcgc cggcatcgcc gatcgatggc aagccctggt atgtctcgcc
      181 gatgttctcc tacctgctgg ccgacaagga ccgcggcacc gatgacggct acggcggcac
      241 gatcaccgtg ggcaagcgcc tcacctacgg cctcaacctg gaactgtccg gcacctacct
      301 gcaggccaag ggcaagaacc agccgggcgt gatcgacaac ggcaccgcca agctgtcgga
      361 tgtcgcggtc accgcgctga tcttcccgat gaccacgcac ccgaacttct tcggcatcct
      421 gtcggtgggc atgagcggcg ccaagaatca cccgggcacc attgccgaat accgcggcac
      481 cgagttcgac gcgggtctgg gctacctgtt ccccatcacc aagaagatcc tgctgcgtgc
      541 ggaagcgcgc taccggctgg atcagagcaa ccggcacgac gccggcgtgc aggtgccgcc
      601 gaagaacaat tccgaattct atgaagggct gttcaacgtc ggcctgctga tcccgctggg
      661 cagcgtcgcc gccccgcccg ccgcgccgcc gcccgaagtg gcgacgccgc cgaccaacga
      721 cagcgatggc gacggcgtgc ccgacgacaa ggacaagtgc ccgggtaccc ccgccggcgc
      781 catcgtcgac gagaacggct gcgagaagga cagcgacggc gacggcgtgc ccgaccgtct
      841 cgacaagtgc ccggatacgc ccgccggcca gaaggtcggt gccgatggct gcccgctgga
      901 cagcgacggc gacggcatcc cggatgacgt cgacgaatgc ccgcacacgc ccgccggcgc
      961 caaggtgctg gccaacggct gcgcgctcac cggcgactgc cgtacgccgc gccccggcga
     1021 gcaggtggat gagaacggct gcgcggtcga caagaccttc atcctcaagg gcgtgaactt
     1081 cgagttcgat tccgaccgcc tgaccgagcc gtccaagcag atactcgacc agaccgcgga
     1141 aacgctgaag tcctatccgg acatcaaggt cgaggtggca ggtcataccg acaacatcgg
     1201 cagtgactcg tacaacctcg gcctgtccga gcgccgtgcc atcgcggtca agaattacct
     1261 gaccggccgc ggcgtggatg cggcgcgcat gagcccgaac ggctacggcc tgacgcagcc
     1321 catcgacagc aacgactcgg aagcgggccg cgccaagaac cgccgcgtcg aactgcgcgt
     1381 gatcgagcag cagaacgcca acgtcggcaa gtaggattcc tgcaacatct gcaaccccgc
     1441 cggagcgatc cggcggggtt ttttattgac cggcacgaat gctgctagac ctagtggtgg
     1501 attgaacctt tgcgcgggcg gcacggtcgg actggaacgc ccgcatcacg cgcggcgttt
     1561 gcatgcacaa gcgccaggga ggccgccatg tacaagaacc ggacgatcac caagcatcgc
     1621 accctgtggg tttccgacgt gcatctgggt tcgcccgggg ccaaggccag ggagctcacc
     1681 gatttcctca agcgcaacga ctgcgaacag ctctacctgg tcggcgacat cttcgacggc
     1741 tggaagatga agtcgcgctt ctactggacg ccggatcact cgcgcgtgat caaggccatc
     1801 atttccaagg cgcgccgcgg caccaaggtg tattacctgg ccggcaacca cgatggcttc
     1861 atgcgccagt tcgtcaagaa ccagttgcgc ctgggcaagg tgcgcatcgc ccatgaagtg
     1921 gtgcacacca ccgccgatgg ccgccgcctg ctggtgacgc acggcgacac cttcgacgac
     1981 gtgatcaacg gcctgccgtg gctggcctac gccggcgatg tcgcctacga ggcgctgatg
     2041 gaagccagca agctgctcaa ccgcgccggt gcgcgcatgg gcctgcccta ctggtcgctg
     2101 tcggccttca ccaagaccgc ggtcaagacg gccgtgcagt actggtcggg cgtggacaac
     2161 aagatgctgc accgctgccg cagcgaaaat ctggccggcg tggtttccgg ccatacccac
     2221 catgcggaga tccgcatgct gcggcccggg atcacgctgc acaactgcgg cgactgggtc
     2281 gaatcctgca ccgcgctgac cgaagaccgc gacggcaaca tcgagatcgt caattactgt
     2341 ccgcagccat ccaacgccgg cgcagcggaa gcggccattg ccgcggtgtc cgcaccgccg
     2401 ccatgggtcg gcgccgtctc gcgcgcagcg aaaaacctgg gcggcatcgg ccgtcgcgcg
     2461 gtgcgccgct cttcgcgcaa gccagcgcgc gtgcgcacgg cgacagcagc cacagacgtg
     2521 gccgtcgccg ccggagagtg atggcgcatc cgcctgtcgc cggcccatgg ccggggtatg
     2581 aaatacccat cgccgggtga gccaagttct gtgagtatcg ctggcaaccg cagcgcgcct
     2641 tgcgctgcgg cgagaaggct cttgcgatgc ggaagttccg ggggttgcgg tctgcttggg
     2701 tcaggtccgg cctgctggcg ttcgcttcat gcctgtgcgg catcgcgagt gccggcgtgg
     2761 atataccgct ggacaagcgt ccggccggca ctttctacac gggcggcgtg atccagggtt
     2821 acggccggct cgacatgctg gtcgataccg gctcgagcat cctagtcatc gacgaaaaca
     2881 tcctcagcca gctggtgcag gccggtgagg ccgagtacag ccgccagctg ggcgggctga
     2941 tggccgacgg cacgcagcgc catgttcccg tgtaccgggt acatgccctg cgcgtcggcg
     3001 acaactgctg gatacgcaat gtggaagccg ccgtgttccc ggcaggttcg cgcgccatcc
     3061 tgggcatgaa tgtgctgacg cgcctggcgc cgttcacctt ttccgccgat ccgccgcagc
     3121 tccagctcaa ccagtgcctg gcgacgcccg cagaagccat gggcggaacc ggcggcgcgc
     3181 cggtggcggc atcccgctag gccgccgagc gggcctgatc gcgcttgccg ctagtggcga
     3241 ctggtgtcgg tgctggtacc ggtggactcg aactgcgaga actccatcgt gtcgagcttg
     3301 ccgtcgtggt tcaggtcaag cgagtcgaat ttcttcccca gcatcttgtc cttcgccgct
     3361 tccttcttgg tgatcatgcc gtcgccgtcg gcatccacct tggcgaaggc gcccgaactt
     3421 ccgctggtct gcacgctgcc cgaagcgccg gccgtgccgg tggtgctcgt ggtgcccggg
     3481 tagccgctgc ccgaatatcc gctgttcgcg ccgccggcgg tggcgctgcc ggaattctgc
     3541 agggtgctgt tggtggaact gttgctgccc ggcgtcaccg tgttgccggc ggccagggct
     3601 gcacccgcga acgatgcgag ggcgatgacg aagctgagct tgcgcatggg aagatcctct
     3661 gtagacggcc gccgacgtgg gatcggcagc aagggccgcg ggatgtggag gcgatcccgt
     3721 cgcaaaattc gacgcgcctc accggcgacg gttcatcccg ccggagcggc cgacaggctt
     3781 gcaacaaatt catcgcgcca gcggcgcacg tcgtaggcga gcaggccgcg catgagcgcg
     3841 gcgtggcgct cgcgccgctc ggccagcggc atgaccaggg cctggtgcag cgcctcggcc
     3901 atcgccgcct cgtcataggg attcaccagc agcgcctcat gcatctgctg cgcggcgccg
     3961 gcgaacctgg acagcaccag cacgcctgga tctgccgggt cctgcgccgc cacgtactcc
     4021 ttggccacca ggttcatgcc gtcgcgcacc ggcgtgacca gaccgacgcg gctggcgcgg
     4081 tacaacccgc cgagcgcgcg ccgcggcagc gcgcggttga ggtagcgcac cggcgtccag
     4141 tcggcctgcg catagcgacc gttgatgccg gcggcggcat gctcgagttc gcgccggaaa
     4201 tccttgtagg ccttgagctc gccgcggctg atcggcgcga tctgcagcag ttccacctgc
     4261 cggtgcagcg ccggataccg ctcaagcagc agttcgtagc cagccatgcg gcgcaacaag
     4321 cccttgctgt aatccaggcg gtcgacgccg atgaccagcg cccggccatt gagcgcctcg
     4381 cgcatgcggc cgccctcgcg catgccctcg gcggacatgg ccaccttctg gaattgggcg
     4441 gcgtcgatgc caatgggaaa cgcacgcaac accagccgtc gcccttgcac acgcagctgc
     4501 tccccgtcga cggcaccacc ggcgtagcgc tgcacataac cctgcagccg ctccacgtca
     4561 ggctgcgtct gcaggccgac cacgtcgtat tggaacagcg cctgcatcag ttcctggtgg
     4621 ccgggcaggg tctcgagaat gtccgccggc ggaaacggcg tgtgcaggaa aaacccgagc
     4681 ctttgcctgg cgccaagctt gcgcagttct gcgccgagcg gaatgaggtg atagtcgtgc
     4741 ac
//
LOCUS       DASZZC010000554         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_106641,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000554 DASZZC010000000
VERSION     DASZZC010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106641"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..669)
                     /locus_tag="VHE37_11775"
     CDS             complement(58..669)
                     /locus_tag="VHE37_11775"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HVT36260.1"
                     /translation="MNFLGHLWLADRTGTSPAGAVLGDVVRGADLSAYGGELALGIRL
                     HRRVDALTDRHRLIAQARTAFPDGARRYAGIVLDVALDHALALDWTQHHALPLHRFCA
                     QAARAVVRDAAAFAAAGAPAPREAGFTALLLSYAEPGGIDHALRRIAARMRDPAPLLQ
                     AATRWPQAARELHGALPDLLGDLHLGAGEFLRAQAAAPAPPSS"
     gene            705..1349
                     /locus_tag="VHE37_11780"
     CDS             705..1349
                     /locus_tag="VHE37_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HVT36261.1"
                     /translation="MRKKAAAADDFPGAQEFLPAQPTLPAMSAAVQDCRGCPLYAHAT
                     QAVFGEGKESAKLVLIGEQPGNDEDLAGQPFIGPAGKLLDRALVAAGIERAAVYVTNA
                     VKHFKFEPRGKRRLHSRPGAREIKACLPWLQQELELIRPLVIVCPGATAAQALLGKTF
                     RLTQHRGEVVSSKWSPRTLVTAHPSSILRQRTHQERERAFSEFVSDLKAATAFL"
     gene            1481..>2818
                     /locus_tag="VHE37_11785"
     CDS             1481..>2818
                     /locus_tag="VHE37_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36262.1"
                     /translation="MKNLLIGGVLGLVCATAAAQDAATVPVPQPAPAPLATDSVATQL
                     DEVQVTAQRKVSTVQETPISIEAFNAEKLSLRGISGLDGLATQVPSLTIEPFPTHNAT
                     LRIAIRGIVNNDAQVTQDPAVGVYLDGVYIARAVGLAIDTADLERIEVLRGPQGTLYG
                     RNTTGGAVNLVTRRPQPGLFSLQQSLTLADRHQALARTMLNLPLGDSFALKLAAIGNQ
                     RDGWVQNSGPGGDFGDRHERALRADARWLAADWLTADYSYDRTDTQYFNYMFQAVLLP
                     QTNHGQAELFKPFAVQNTVYSSQRLDTLATAMPLDPSGSHIQGHALVLTAPLGAAELK
                     YIGAYRRLTDDEYTDLSGGNGSPDFRVDTEAYDGPSATLANGGPVGAMTPQVFQRQWS
                     HELQLSGKFDDGAVEFIAGAFHFLEKGGENGRPLHHILNTELDPQQAQPYFSLV"
BASE COUNT          480 a          974 c          987 g          377 t
ORIGIN      
        1 acgaccgcaa agtgaaggcg ccgcgcgatg cggcagcctt gatggacgag ctcgatctca
       61 gctggatggc ggggcaggcg cagccgcctg cgcgcgcagg aactcgccgg cgccgagatg
      121 caggtcgccg agcaggtccg gcaacgcgcc gtgcaattcc cgcgcggcct gcggccagcg
      181 cgtagcggcc tgcagcagcg gcgcgggatc gcgcatgcgc gcggcgatgc ggcgcagcgc
      241 gtgatcgatg ccgccgggct ccgcgtacga cagcagcagg gcggtaaagc ccgcttcgcg
      301 cggcgccggc gcgcccgcgg cggcgaaagc ggcggcatcg cgcaccactg cgcgcgcggc
      361 ctgcgcgcag aaccggtgca ggggcagcgc atggtgctgc gtccagtcga gcgccagcgc
      421 gtgatcgagc gccacgtcga gcacgatgcc ggcgtagcgc cgcgcgccat cggggaacgc
      481 ggtgcgcgcc tgcgcgatca gccgatggcg gtcggtgagc gcatcgacgc gccggtgcag
      541 gcggatgccg agcgcgagct cgccgccata ggcagacaga tcggcgccgc gcaccacgtc
      601 gcccagcact gcgccggccg gcgaagtgcc cgtgcggtcg gccagccaga gatgcccgag
      661 gaaattcatg cgcaacgcag cgtgcgggtg tctatgcact ttagatgaga aagaaagccg
      721 cggctgccga tgattttccc ggcgcgcagg agttcctgcc ggcacagccg acgctgcccg
      781 cgatgtccgc agccgtgcag gactgccgcg gctgtccgct ctacgcccat gccacgcagg
      841 cagtgttcgg cgaaggaaaa gagtccgcga agctggtgct gatcggcgaa cagccgggca
      901 atgacgaaga cctcgccggg caacctttca tcggccccgc aggcaagctg ctggaccgcg
      961 cactggttgc tgccggcatc gagcgcgccg cggtgtacgt gaccaatgcg gtcaagcatt
     1021 tcaagttcga gccgcgcggc aagcgccgcc tgcacagcag gccgggcgca cgcgagatca
     1081 aggcctgctt gccgtggctg cagcaggagc tcgagctgat ccgcccgctg gtcatcgtct
     1141 gcccgggggc gaccgcggcg caggcgctgc tcggcaagac ctttcgcctg acgcagcatc
     1201 gcggggaagt agtctcatcg aaatggtcgc cacggaccct ggtgacggcg catccatcgt
     1261 ccatcctgcg ccagcgcacg caccaggagc gcgagcgcgc gttcagcgaa ttcgtgtccg
     1321 acctcaaagc ggcgactgcg ttcctgtagg agcgccttgc gcgcgatgcg gtcgagccag
     1381 gaggaaagcg catcgcgggc aaggcccgct cctacaaaag ccggcagcgg ctggcatcat
     1441 aggatcaccg cgcaacaaga cgcggcgagg ggagaacggc atgaagaacc tgctcatcgg
     1501 gggcgtactc ggtttggttt gcgcaacggc ggcggcgcag gacgccgcca ccgtgccggt
     1561 gccgcagccg gcgcccgcgc cgcttgctac ggattccgta gcaacgcagc tggacgaggt
     1621 gcaggtgacg gcgcagcgca aggtgtcgac cgtgcaggag accccgattt ccatcgaggc
     1681 cttcaatgcc gagaagctgt cgctgcgcgg catcagcggt ctggacggac tggccacgca
     1741 ggtgccgagc ctgaccatag agccgtttcc gacgcacaac gccacgctgc gcatcgccat
     1801 ccgcggcatc gtcaacaacg acgcgcaggt gacgcaggac ccggcggtgg gcgtgtacct
     1861 cgacggcgtg tacatcgcgc gcgcggtcgg cctggccatc gacaccgccg acctggaaag
     1921 gatcgaggtg ctgcgcggcc cgcagggcac gctgtacggg cgcaacacca ccggcggtgc
     1981 ggtcaacctg gtgacgcgcc gcccgcagcc cggcctgttt tcgctgcagc agtcgctgac
     2041 gctggccgac cgccaccagg cgctggccag gaccatgctc aacctgccgc tgggcgacag
     2101 cttcgcgctc aagctcgcgg ccatcggcaa ccagcgcgac ggctgggtgc agaacagcgg
     2161 ccccggcggc gatttcggcg accgccacga gcgcgcgctg cgcgccgacg cgcgctggct
     2221 ggccgccgac tggctcaccg ccgactacag ctacgaccgc accgacacgc agtacttcaa
     2281 ctatatgttc caggcggtgc tgctgccgca aaccaaccac ggccaggccg agctgttcaa
     2341 accctttgcc gtgcagaaca cggtgtactc gtcgcagcgg ctggatacgc tggccactgc
     2401 catgccgctc gatccgtcgg gctcgcacat ccagggccac gcgctggtgc tgaccgcgcc
     2461 gctgggcgcg gcggaactga aatacatcgg cgcgtaccgg cggctcaccg acgatgaata
     2521 caccgacctg tccggcggca acggctcgcc cgatttccgc gtcgacacgg aagcgtatga
     2581 cggcccctcc gccacgctgg ccaatggcgg gccagtgggc gcgatgacgc cgcaggtgtt
     2641 tcagcgccag tggtcgcacg agctgcagct gtcgggcaag ttcgatgacg gcgcggtgga
     2701 gttcatcgcc ggcgcgttcc atttcctgga gaagggcggc gagaacgggc ggccgctgca
     2761 ccacatcctc aacaccgagc tcgacccgca gcaggcgcag ccgtacttct cgctggtg
//
LOCUS       DASZZC010000555         4197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1012405,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000555 DASZZC010000000
VERSION     DASZZC010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012405"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2265
                     /locus_tag="VHE37_11790"
     CDS             <1..2265
                     /locus_tag="VHE37_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36263.1"
                     /translation="VPASVSTVDGEFIRKSGVANFDALQDYAPNVTISLGATSGVFAI
                     RGFATPDTNPGFDPSVGAVVDGVYYGRSQFMDAFFADLDRFEVLRGPQGSLFGKNSTA
                     GVLNVVTQAPQPEAMAVAEVQQSSFGDTSIRPVIQLPLGSTFSLRYAGNYAHGDKGVL
                     YNTFLQRKEEEIIKDSSRLRARWTPDAYFTLDLEGFYSKSHSNNNGFQFSTITPRMQA
                     LVQSYDPADNATVDRYNSASYPSLMASTIEGANMTVSDDVSELTNIDDAKVTLITSHA
                     QARTTARDLDADFTPVNFIHDTLAEPSGYTQTSEELRLSGHGKSMGGFGHGYNFVGGV
                     FMSGSNFHSSDLFAVEDLGAALAYCNATPDFCGLKVPAVIPPGVVGQVGQTLGNTLDQ
                     VAALAGQGNQSATVKLNQQGSDFAVFGQFEHFFLPQWALIGGLRYGIEKKTGTAYSHS
                     ASPLVKQIANQAELDNVPLHRIEHDLSPKAGIKWEGDETQSAYLTWARGYKSGGFNAL
                     PLSGDHLEYEPEQATSIEAGGKALTHFLGGPLRLSVAVFRTDFDNLQVSTFLGGDFIV
                     LNAAAARSQGFDLDLHWLTPLRGLSITAQAGLADAKYKSYPCAPAIADSTAPGNPSCT
                     GKSNDTTTQDLTGKPLSFSPRWTAGFIPAWQFSPLDTLSTTLALDLLYRGSRYLDVDL
                     DPRKVQAATTLINGRVVLETPSKRWGLSLVAHNLTDKVFGDQAISQPLAPGNVLLYHT
                     DRGRYVTGDVTVQF"
     gene            2300..>4197
                     /locus_tag="VHE37_11795"
     CDS             2300..>4197
                     /locus_tag="VHE37_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36264.1"
                     /translation="MWLIAALLAAPGARADDAQELPSVIVTSQKIAQSLEEVPASVST
                     VDGETIRATGAHSLTDMQDYIPNVSIAQNHTAATIAIRGFATPDTNPGFDPSVGAVVD
                     GIYYGRSQFLTAFFHDMDRIEVLRGPQGTLFGKNSTAGVMSIVTQAPGNEWLVQAEAQ
                     QTSFGELSLQPVLQIPLGQDLSIRVSGDYRHGSKGVLYNTFLQRPENNPQSDSTRLRL
                     RYAPDTLISFDFEAFTSREKFNNNGFQNSVMTARMRQALMQYDPAIDSNIDKYNSQSF
                     PSLSDGVIRGLSGTFTFNIDGQMGVPDLKVLTIIGVADSITSKKELDADFSPIDFLDN
                     TQAAPSYYKQLTDEIRLVGQGQKIFGFGHGYNFVTGFFLSSNSFRDSGIYRVEDLGGA
                     AAYCNATSDFCGVPIPGILPGSVGSDLGLTLGPVLNAFTSLAGGAADQTAHLNLDQTG
                     HSWAFFGQTEAFLLEHWALIGGLRYGDEVKDGFASSHSTSPLVKAITSQVDHDTPLHL
                     EERDISPKAGFKWEPDKRLTAYATWSRGYKSGGFNALPLAPTNLQYQPERAESFEIGS
                     KGITEFLGTPVRLSAAVYDTSFDNLQVSAFLNGNFVVLNAASARSRGLDLDFHWLTPV
                     TGFSVTGA"
BASE COUNT          744 a         1498 c         1284 g          671 t
ORIGIN      
        1 gtgccggcct cggtcagcac ggtggatggc gaattcatcc gcaagtccgg cgtggccaac
       61 ttcgacgcgc tgcaggacta cgcgcccaac gtcaccattt ccctgggcgc gacctcgggc
      121 gtgttcgcca tccgcggctt cgccacgccg gacaccaatc ccggcttcga tccttccgtc
      181 ggcgcggtgg tcgatggcgt ctactacggg cgctcgcagt tcatggacgc gttcttcgct
      241 gatctcgacc gcttcgaggt gctgcgcggc ccgcagggtt cgctgttcgg caagaacagc
      301 accgccggcg tgctcaacgt ggtgacgcag gcgccgcagc ccgaagcgat ggcggtggcc
      361 gaagtgcagc agtccagctt cggcgacacc tcgatccgcc cggtgatcca gctgccgctg
      421 ggcagtactt tctcgctgcg ctacgccggc aactatgcac acggtgacaa aggcgtgctg
      481 tacaacacct tcctgcagcg caaggaggag gagatcatca aggacagcag ccgcctgcgc
      541 gcgcgctgga cgccggacgc ctacttcacg ctggacctgg aaggcttcta ttcgaagtcg
      601 cactccaaca acaacggctt ccagttctcc accatcacgc cgcgcatgca ggcgctggtg
      661 caaagctacg acccggccga caacgcgacg gtcgaccgct acaactccgc cagctatccg
      721 tcgctgatgg cctcgaccat cgagggcgcc aacatgaccg tgtcggacga tgtcagcgag
      781 ctcaccaaca tcgacgacgc caaggtcacg ctgatcacct cgcacgcgca ggcgcgcacc
      841 accgcgcgcg atctcgacgc cgatttcacg ccggtcaatt tcatccatga cacgctggcc
      901 gagccgtcgg ggtacacgca gaccagcgaa gagctgcgcc tgtcgggcca tggcaagtcc
      961 atgggcggtt tcggccacgg ctacaacttc gtcggcggcg tgttcatgtc gggcagcaac
     1021 ttccactcgt cggacctgtt cgccgtggaa gacctgggcg cggcgctggc ctactgcaat
     1081 gccacgccgg atttctgcgg actgaaagtg ccggcggtga ttccgcccgg cgtggtggga
     1141 caggtgggac agacgctggg caacacgctg gaccaggtgg cggcgctggc cggccagggc
     1201 aaccagtcgg ccacggtcaa gctcaaccag cagggcagcg atttcgccgt gttcggccag
     1261 ttcgaacatt tcttcctgcc gcagtgggcg ctgatcggcg gcctgcgcta cggcatcgag
     1321 aagaagaccg gcaccgccta cagccactcg gccagcccgc tggtcaagca gatcgccaac
     1381 caggccgagt tggacaacgt gccgctgcat cgcatcgagc atgacctgtc gcccaaggcc
     1441 ggcatcaagt gggaaggcga cgagacgcag agcgcctacc tgacctgggc gcgcggctac
     1501 aagagcggcg gcttcaacgc gctgccgctg tcgggcgatc acctcgagta cgagccggag
     1561 caggccacca gcatcgaggc cggcggcaag gcgctgaccc atttcctcgg cgggccgctg
     1621 cgcctttcgg tggccgtctt ccgcaccgac ttcgacaacc tgcaggtgtc gaccttcctc
     1681 ggcggcgact tcatcgtgct caacgccgcc gcggcgcgct cgcagggctt cgacctcgac
     1741 ctgcactggc tgacgccgct gcgcggcctg tcgatcaccg cccaggccgg cctggccgat
     1801 gccaagtaca agtcgtaccc gtgcgcgccg gccattgccg actccaccgc gccgggcaac
     1861 ccgtcgtgca ccggcaagag caacgacacc accacgcagg atctcaccgg caagccgctg
     1921 tcgttctcgc cacgctggac cgccggcttc atcccggcct ggcagttcag tccgctggac
     1981 acgctgtcga ccacgctggc gctggacctg ctgtaccgcg gctcgcgcta cctcgacgtc
     2041 gatctcgatc cgcgcaaggt gcaggcggcc accacgctga tcaatggccg cgtggtgctg
     2101 gaaacgccga gcaagcgctg gggcctttcc ctggtggcgc acaacctgac cgacaaggtc
     2161 ttcggcgacc aggccatcag ccagccgctg gcgccaggca atgtgctgct gtaccacacc
     2221 gaccgcggcc gctatgtcac cggcgacgtg accgtgcagt tctgatcctc accgagcaac
     2281 cgccatcgac accaagaact tgtggctcat cgcggcgctg ctggccgcac ctggcgcgcg
     2341 cgcggacgac gcgcaggaac tgccgtcggt catcgtcact tcgcagaaga ttgcgcagtc
     2401 gctggaggaa gtgccggcct cggtcagcac ggtggatggc gagaccatcc gcgccaccgg
     2461 cgcgcacagc ctcaccgaca tgcaggacta catccccaac gtcagcatcg cgcagaacca
     2521 caccgcggcc accatcgcca tccgcggctt tgccacgccg gataccaacc cgggcttcga
     2581 cccctcggtc ggtgcggtgg tcgacggcat ctactacggc cgctcgcagt tcctcaccgc
     2641 gttcttccac gacatggacc gcatcgaggt gctgcgcgga ccgcagggca cgctgtttgg
     2701 caagaacagc accgccggcg tgatgtcgat cgtgactcag gccccgggca acgaatggct
     2761 ggtgcaggcc gaagcgcagc agacctcgtt cggcgaactg tcgctgcagc cggtgctgca
     2821 gatcccgctg ggccaggacc tgtcgatccg cgtctccggc gactatcgcc acggcagcaa
     2881 gggcgtgctg tacaacacct tcctgcaacg gccggagaac aacccgcagt cggattccac
     2941 gcgcctgcgc ctgcgctacg cgcccgatac gctgatcagc ttcgacttcg aggccttcac
     3001 ctcgcgcgaa aaattcaaca acaacggatt ccagaactcg gtgatgaccg cgcgcatgcg
     3061 ccaggcgctg atgcagtacg acccggccat cgacagcaac atcgacaagt acaactcgca
     3121 gagttttccg tcgctgtccg acggcgtgat ccgcgggctg tccggcacct tcaccttcaa
     3181 catcgacggc cagatgggcg tgcctgacct gaaggtgctg accatcatcg gcgtggccga
     3241 ctccatcacc agcaagaagg aactcgacgc cgacttcagc ccgatcgatt tcctcgacaa
     3301 cacccaggcc gcgcccagct actacaagca gctgaccgac gagatccgcc tggtcggcca
     3361 gggccagaag atcttcggtt tcggccacgg ctacaacttc gtcaccggct tcttcctgtc
     3421 gtcgaactcg ttccgcgact ccggcatcta ccgggtggaa gacctgggcg gcgccgcggc
     3481 ctattgcaac gccacctccg atttctgcgg cgtgccgatt cccggcatcc ttcccggcag
     3541 cgtcggcagc gatctcggcc tgacgctggg gccggtgctg aatgccttca ccagcctggc
     3601 cggcggcgcg gcggaccaga ccgcgcatct caacctcgac cagaccgggc actcatgggc
     3661 tttcttcggc cagaccgagg ccttcctgct cgagcactgg gcactgatcg gcggcctgcg
     3721 ctacggcgac gaggtgaagg acggtttcgc ctcgagccat tccaccagcc cgctggtgaa
     3781 ggcgatcacc tcgcaggtcg accacgacac gccgctgcac ctggaagagc gcgacatctc
     3841 gcccaaggcc ggcttcaagt gggagccgga caagcgcctc accgcgtacg ccacctggag
     3901 ccgcggctac aagagcggcg gcttcaacgc gctgccgctg gccccgacca acctgcagta
     3961 ccagccggaa cgcgcggaga gcttcgagat cggcagcaag ggcatcaccg agttcctcgg
     4021 cacgccggtg cgcctgtcgg cggcggtgta cgacaccagc ttcgacaacc tgcaggtgtc
     4081 ggctttcctc aacggcaact tcgtggtgct caacgcggcc tcggcgcgct cgcgcgggct
     4141 cgacctggat ttccactggc tgacaccggt caccggcttt tccgtcaccg gcgccta
//
LOCUS       DASZZC010000556         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1261096,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000556 DASZZC010000000
VERSION     DASZZC010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261096"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="VHE37_11800"
     CDS             <1..1223
                     /locus_tag="VHE37_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HVT36265.1"
                     /translation="CWEVASYAKELRGAKKQKESVEGLIKASKILGKSFGKAYLNFGE
                     PVILQDYADQHLPGWREQITPNTDDLPAGFKAFVTRLAVENARRTNAAAVANPVGLAA
                     VALLSAPQRAVSSEELVEQIGHLIWLLKGRPYSPYMQVPEAAPRAVVDFAQPIARMVT
                     VPHPWGDLYAIADRDAVALTYARNNVQHLYALPSLIANFFRTRGLLPDDAVVMGVRAL
                     YPFLRTEFFLRWEPEEIEGVTREWIGVMVQLGLLERADDGRLRRPDVNQTAFSTLATL
                     GRVMSETLERYCMTALLLAEERNNGGGLKRGRFEDDCRLLAERMAILTGRDAPEFFDK
                     ALFKGYLNTLIDIGLVGESDEAGLEVDGRIERIAERSLELLSDESRQTLLQLLSRRKT
                     PVSGQVSPPGAPTL"
     gene            1270..>1974
                     /locus_tag="VHE37_11805"
     CDS             1270..>1974
                     /locus_tag="VHE37_11805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HVT36266.1"
                     /translation="MYKSIRLLAALAAAGMIAACAQKAGDNSTAGASGDLSTDAQKFG
                     YAIGVDLAKSLAPVKDSVDLAALKQGLDDASSGATPKMDDKARQQIKETVAKKMQEKQ
                     MAERAEQAKKLQEEGEKFLAENAKKEGVKTTASGLQYEVITEGKGPHPTADDTVKVHY
                     KGTLLNGEQFDSSYDRGQPVDIPLKNVIPGWTEGVQLMTPGSKYRFFIPSKLGYGERG
                     AGVKIGPNATLIFEVEL"
BASE COUNT          375 a          645 c          665 g          289 t
ORIGIN      
        1 agtgctggga ggtggccagc tatgccaagg aactgcgcgg cgccaagaag cagaaggagt
       61 cggtggaagg gctgatcaag gccagcaaga tcctgggcaa gagcttcggc aaggcctacc
      121 tgaatttcgg cgagccggtg atcctgcagg actacgccga ccagcacctg cccggctggc
      181 gcgagcagat cacgcccaat accgacgatc tgccggccgg gttcaaggcc ttcgtcaccc
      241 ggctggcggt cgagaatgcg cggcgcacca acgccgcggc ggtggccaac ccggtggggc
      301 tggcggcggt ggcgctgctg tcggcgccgc agcgcgcagt gtcgagcgaa gaactggtgg
      361 agcagatcgg tcacctgatc tggctgctca agggccggcc gtacagcccg tacatgcagg
      421 tgccggaagc ggcgccgcga gcagtggtgg actttgccca gccgatcgcg cgcatggtca
      481 ccgtaccgca cccgtggggc gatctctacg ccatcgccga ccgcgatgcg gtggcgctca
      541 cctatgcgcg caacaacgtg cagcacctgt acgcgttgcc gtcgctgatc gccaacttct
      601 tccgcacccg cggcctgctg cccgacgacg ccgtggtgat gggcgtgcgc gcgctgtacc
      661 cgttcctgcg caccgagttc ttcctgcgct gggagccaga ggaaatcgag ggagtgacgc
      721 gcgaatggat cggcgtgatg gtgcagctgg gcctgctgga gcgcgccgat gacggccgcc
      781 tgcgccggcc cgacgtcaac cagaccgcgt tctccacgct cgccacgctg ggccgggtga
      841 tgagcgagac gctggaacgc tactgcatga ccgcgctgct gctggccgag gagcgcaaca
      901 acggcggcgg cctcaagcgc ggccgcttcg aggacgactg ccgcctgctg gccgagcgca
      961 tggccatcct taccggccgc gacgcgccgg agttcttcga caaggcgctg ttcaagggct
     1021 acctcaacac gctgatcgac atcggcctgg tgggtgaaag cgacgaggcc ggactggagg
     1081 tggacgggcg catcgagcgc atcgcggaac gttcgctgga actgctgtcg gacgagtcgc
     1141 gccagaccct gctgcagctg ctgtcgcgcc ggaaaacgcc ggtttccggc caggtgtccc
     1201 ctcccggcgc tcctacacta taatcgcgcg cctttccaac accccacata aattcaggac
     1261 tctcatcaaa tgtacaaatc catccgtctg ctggccgcgc ttgccgccgc cggcatgatt
     1321 gccgcctgcg cccagaaggc cggcgacaac agcactgccg gtgcttccgg cgatctttcc
     1381 accgacgcgc agaagttcgg ctatgccatc ggcgttgacc tggccaagtc gctggcgccg
     1441 gtgaaggaca gcgtcgacct ggccgcgctc aagcagggcc tggatgacgc gtccagcggc
     1501 gccacgccga agatggacga caaggcgcgc cagcagatca aggaaaccgt cgccaagaag
     1561 atgcaggaaa agcagatggc cgagcgcgca gagcaggcca agaagctgca ggaagaaggc
     1621 gagaagtttc tggccgagaa tgccaagaag gaaggcgtga agaccaccgc ctcgggactg
     1681 cagtacgaag tgatcaccga gggcaagggc ccgcacccga ccgccgacga cacggtgaag
     1741 gtccactaca agggcaccct gctcaacggc gagcagttcg actcctcgta cgatcgcggc
     1801 cagccggtgg acatcccgct gaagaacgtg atcccgggct ggaccgaggg cgtgcagctg
     1861 atgacgccgg gctccaagta ccgcttcttc atcccgtcga agctgggcta cggtgagcgc
     1921 ggcgccggcg tgaagatcgg ccccaacgcc acgctgatct tcgaagtcga gctg
//
LOCUS       DASZZC010000557         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_49056,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000557 DASZZC010000000
VERSION     DASZZC010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49056"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1032)
                     /locus_tag="VHE37_11810"
     CDS             complement(127..1032)
                     /locus_tag="VHE37_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HVT36267.1"
                     /translation="MSLHRPDWRAAGVALAAALCACGGGGSDATPVAPVDLGPPTWSK
                     EFANGNGVTPISSGNGSVTFGAAGGADDGNAASLLFPGDSSKGPNARAGTGYASEIDS
                     VKGDFHYGEYRARLRLATCAPTEELVNGYFTYFHDGNDYNGNGIADNSEIDIEIQCGR
                     PYAIFLTSWTDYDSETSFRKWTRVIDTSTGDIWESVADDQFDIHKIGNDPAFLIPGFP
                     DANTFYEMGFDWEPDHIRWFLVNGGQEITLWNFTNAQLVPNLPATLIFNLWHANSHWF
                     AGGSAADYPAADGTLTVDWARYWQQ"
     gene            complement(1013..2047)
                     /locus_tag="VHE37_11815"
     CDS             complement(1013..2047)
                     /locus_tag="VHE37_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVT36268.1"
                     /translation="MPQPSNRRFLLRQRPLGDFDPKILERVVQPAPTPGPGQALVRNL
                     YLSLDPTNRIWMSEAEGYLPPVALGEVMRGAAVGKVVASNSKKFPAGATVAGLLGWQD
                     YALLSDDGDTVAQVIPNWLPVPLTATMGVLGATGMTAYFGLTDIGKPQPGETVVISAA
                     AGAVGSIAGQIAKLLGCRVVGIAGTREKCEWLTRELGFDAAINRRDADWRAQLKAACP
                     NGVDVDFENAGGEILDAVLDLMNLHGRVILCGMISQYGSDGAVAGPKNFRHLLIRRIK
                     VQGFIILDYIPKFPLAQLRMLWWLKRGKIKDRSTIVKGLDQAPQALTQLFRGDNTGKL
                     IVEIDEPPPA"
     gene            complement(2167..2607)
                     /locus_tag="VHE37_11820"
     CDS             complement(2167..2607)
                     /locus_tag="VHE37_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HVT36269.1"
                     /translation="MRGRPPKSPVVPSRKIWVDSECCSRGIIDQLLRMAHERRIILTL
                     VSSRAQAHSHEHSPYVRTLVVSSEFGDAPARIAAMIDGGDIVVTSNAGLASTAGQKSA
                     TALNPGGGARGERQAPDDTFSPSGDTAHASLPLAARIVELLAQK"
     gene            complement(2604..2999)
                     /locus_tag="VHE37_11825"
     CDS             complement(2604..2999)
                     /locus_tag="VHE37_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36270.1"
                     /translation="MKSSPTIHAGMTAAGFRRARYGDFVVITLSNGAFAVTAQDMMMV
                     ETWARGRTSFGNAQRDQTAFTDRFDNVLARSGSGIATKGSRPVLARIVAGMMANGIDI
                     KEWSVPHNINESVEVVRRKPPVNPLEPPA"
     gene            complement(3188..3400)
                     /locus_tag="VHE37_11830"
     CDS             complement(3188..3400)
                     /locus_tag="VHE37_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVT36271.1"
                     /translation="MSNVSTGSVKWFNEAKGFGFITPSEGGEDLFAHFREIQGEGFKT
                     LTEGQKVEYIANRGPKGMQATRIRPL"
     gene            complement(3591..3842)
                     /gene="rpoZ"
                     /locus_tag="VHE37_11835"
     CDS             complement(3591..3842)
                     /gene="rpoZ"
                     /locus_tag="VHE37_11835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HVT36272.1"
                     /translation="MARVTVEDCLDQVPNPFILTRIAAARARQLARGASSELPWGDHK
                     STVLALQEIAAGRVTEAVLDELELPLPKPPGEVFEPLPE"
     gene            3984..>4901
                     /locus_tag="VHE37_11840"
     CDS             3984..>4901
                     /locus_tag="VHE37_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVT36273.1"
                     /translation="MGMIRVALALAVLFTHLPIAPFRVMGGGLAVQSFFIISGFYMAL
                     VLEGKYADRALFYSNRLLRLAPTYFVMLVLSAIALFVFSLSATASPELFADAFGHPGS
                     ALLLGFENLAVFGQEMLFWFRLGSDGLVFDASGALPGETSSVAWQALLVPQSWSLSME
                     LMFYLLAPWLVRLRTRTLFVIAAASIGLRFAGHALPVDYGIWQGRLFPTALFLFVLGM
                     LAQRALPRAAQLPRAGGYAGALVLLALIALLPQLHLPDEPSRWLMYSCVAAVAPLAFS
                     AFRHSAFDRWIGDLSYPIYLCHLLVVGFVL"
BASE COUNT          750 a         1571 c         1685 g          895 t
ORIGIN      
        1 cagcggtgac cagcgcgacg cctgcaagaa gcgcgcggag gccgaccgcg acaccgagaa
       61 ggctgacgcc aaggccgacg agaaggcgca ggacccgaag cgctgaaacg caggcttact
      121 gctgccttac tgctgccagt agcgcgccca gtccaccgtc agcgtgccgt cggccgccgg
      181 gtaatccgcc gcgctgccgc cggcgaacca gtggctgttg gcgtgccaca ggttgaagat
      241 cagcgtggcg ggcaggttgg gaaccagctg cgcattggtg aaattccaca gcgtgatttc
      301 ctggccgccg ttcaccagga accagcggat gtggtccggc tcccagtcga agcccatctc
      361 gtaaaaggta ttggcatcgg gaaaaccggg aatgaggaag gccgggtcgt tgccgatctt
      421 gtggatgtcg aactggtcgt cggcgaccga ttcccagatg tcgccggtgg acgtgtcgat
      481 cacgcgcgtc catttgcgga agctggtttc gctgtcgtag tcggtccagc tggtgaggaa
      541 gatcgcgtac ggccggccgc actggatctc gatatcgatc tcggaattgt cggcgatgcc
      601 gttgccgttg tagtcgttgc cgtcgtgaaa gtaggtgaag tagccgttga ccagttcctc
      661 ggtcggggcg caggtggcga gcctgagccg cgcgcggtat tcgccgtagt ggaaatcgcc
      721 cttcaccgaa tcgatttcgc tcgcgtagcc cgtgcccgcg cgcgcgttgg ggcccttgga
      781 gctgtcgccg gggaagagca gcgaagcggc gttgccatcg tccgcgccgc cggccgcgcc
      841 gaaggtcacc gagccattgc ccgaagaaat cggcgtcacg ccgttgccgt tggcgaattc
      901 cttcgaccag gtcggcggac cgaggtcgac cggcgcaacc ggcgtcgcat cgctgccgcc
      961 accgccgcac gcgcacagcg cggccgcgag cgcaactccc gccgcgcgcc agtcaggccg
     1021 gtggaggctc atcgatttcc acgatcagct tgccggtgtt gtcgccgcgg aacagctgcg
     1081 tgagtgcctg cggcgcctgg tcgagtcctt tcacgatggt gctgcgatcc ttgatcttgc
     1141 cgcgcttgag ccaccacagc atgcgcagct gcgccagcgg aaatttcggg atgtagtcga
     1201 ggatgatgaa gccctgcacc ttgatgcggc ggatcagcag gtggcggaaa ttcttcgggc
     1261 cggccacggc gccgtcgctg ccgtactgcg agatcatgcc gcacaggatc acgcggccgt
     1321 gcaggttcat caggtcgagc accgcgtcca ggatttctcc gccggcgttc tcgaaatcca
     1381 catcgacgcc gttggggcag gcggctttca gctgcgcgcg ccagtcggcg tcgcgccggt
     1441 tgatggcggc gtcgaagccg agctcgcgcg tcagccattc gcatttctcg cgcgtgccgg
     1501 cgatgcccac cacgcggcag ccgagcagct tggcgatctg cccggcgatg ctgcccaccg
     1561 cgccagcggc ggcggagatc accaccgtct cgccgggctg cggcttgccg atgtcggtca
     1621 ggccgaagta cgcggtcatg ccggtggcac cgagcacgcc catggtcgcg gtcagaggca
     1681 ccggcagcca gttcggaatc acctgcgcca ccgtgtcgcc gtcatcggac agcagcgcgt
     1741 agtcctgcca gccgagcagc ccggcgacgg tcgcgcccgc cgggaatttc ttcgagttcg
     1801 acgccaccac cttgccgacg gcggcgccgc gcatcacttc gcccagcgcc accggcggca
     1861 ggtagccttc ggcctcgctc atccagatgc ggttggtcgg atcgagcgac aggtacaggt
     1921 tgcgcaccag cgcctggccg gggcccggcg tgggcgcggg ctgcaccacg cgctcgagga
     1981 tcttcggatc gaaatcgccc aatgggcgct ggcgcagcag gaagcggcgg ttgctgggtt
     2041 gcggcatggt tcccccatat gtcatgcgcg atgcgcatta tgcggcgccg cgccggtggc
     2101 ggcgtgcaag ctgctgctct tcacggcggg acctgcggcg ccggtcatcc tgcaacctgg
     2161 cgcaggctat ttttgcgcga gcagttcgac tatgcgcgcc gccagcggca ggctcgcgtg
     2221 ggccgtgtcg ccggacgggg agaaagtgtc atccggcgcc tggcgctcgc cgcgggcgcc
     2281 gccgccggga ttcagcgcgg tggcgctttt ctgcccggcg gtgctggcca ggccggcatt
     2341 gctggtcacc acgatgtctc cgccgtcgat catggcggcg atgcgcgcgg gcgcgtcgcc
     2401 gaattccgaa gacaccacga gcgtgcgcac gtagggcgag tgttcgtgcg agtgcgcttg
     2461 cgcgcggctg gacaccagcg tcaggatgat gcgccgctca tgcgccatgc gcagcagctg
     2521 gtcgatgatg ccgcgcgagc agcattccga atcgacccag atcttgcgtg acgggacaac
     2581 cggcgatttt gggggacggc ctctcaagcg gggggctcca atggattgac gggcggcttg
     2641 cgccggacga cttcgacgct ttcattgatg ttgtgcggca ccgaccattc cttgatgtcg
     2701 atgccgttgg ccatcatccc ggccacgatg cgcgcgagca ccggtcggct gcccttggtg
     2761 gcgatgccgc tgccgctgcg cgccagcacg ttgtcaaagc gatcagtgaa agcagtctgg
     2821 tcgcgctggg cattgccgaa cgaggtacgg ccgcgcgccc aggtctcgac catcatcatg
     2881 tcctgcgctg ttacggcaaa cgcgccattc gacaaggtga tcacaacaaa gtcgccatag
     2941 cgtgcgcgtc gaaagccggc ggccgtcatg ccggcatgaa tagtggggga ggatttcacg
     3001 tggggctccg ctcgcggtgg ctgcaccaga agcggcccgc ttgcgaagcc ggctgcgtgc
     3061 agggcgaggg gcggtggggc cgatagccga agtggccagt gtgcgccttt tccgcgcgtg
     3121 ccgtacggaa atctgcaggg agtcaaaagg accgcggctg aagccgcggt cccgatcctg
     3181 ccgcggatta aagcgggcgg atgcgcgtcg cctgcatgcc cttggggccg cggttggcga
     3241 tgtactcgac cttctggcct tcggtcaggg tcttgaagcc ctcaccctgg atttcgcgga
     3301 agtgggcgaa aagatcttcg ccgccttcgc tcggggtgat gaagccgaaa cccttggctt
     3361 cgttgaacca cttcaccgat ccggtggaaa cattgctcat gttgcattcc tcgtatgaat
     3421 aagcaggcaa aaagcgcccg cgtgctgcaa gccgtcaagg aacgcatcaa ggagaattcg
     3481 cacgcgaaag gctgcggcgg gagatcagct gaacacactg cttgaaactt tgcgggggcc
     3541 acggtacgcg agtcgacgcc aaaagtcgaa cgcgttcgcg gcgcgacgcg tcactccggc
     3601 aatggttcga atacctcgcc cggcggcttg ggcaacggta gttccagctc gtcgagcacc
     3661 gcctcggtca cccggccggc ggcgatttcc tgcaacgcca gcacggtgga cttgtgatcg
     3721 ccccagggca gttcggacga ggcgccgcgt gccagctggc gcgcgcgcgc ggctgcaatg
     3781 cgcgtgagga tgaacggatt gggaacctga tcgagacagt cttcgacggt gacacgtgcc
     3841 atgggtgact ccggtgaacc accgcagaag cacggtcgct gcactatacg cgcatgcgtc
     3901 gcggccagtg ctggcctgcc ggcgcaggaa ctttccgcac ctgctccggt ccctgctgcg
     3961 ccgatgacaa aaggggaggc ggcatgggga tgatccgcgt ggcactggcg ctggcggtgc
     4021 tgttcacgca cctgccgatt gcgccgttcc gggtgatggg cggcgggctc gcggtgcaga
     4081 gcttcttcat catttcgggt ttttacatgg cgctggtgct ggaagggaaa tacgccgatc
     4141 gcgcgctgtt ctactccaac cggctgctgc gcctggcgcc gacctacttc gtgatgctgg
     4201 tgctgtcggc catcgcgctg ttcgtcttct cgctcagcgc caccgcctca ccggaattgt
     4261 tcgccgacgc attcgggcat ccgggcagtg cgctgctgct gggtttcgag aacctcgcgg
     4321 tgttcgggca ggaaatgctg ttctggttcc gcctgggcag cgatggcctg gtgttcgacg
     4381 ccagcggcgc attgcccgga gagacttcgt cggtggcctg gcaggcactg ctggtgccgc
     4441 agtcgtggag cctgtcgatg gaactgatgt tctacctgct tgcgccgtgg ctggtgcgcc
     4501 tgcgcacgcg cacgctgttc gtcatcgccg ccgccagcat cggcctgcgt tttgccgggc
     4561 acgcgctgcc ggtggattac ggcatctggc agggccggct gtttcccacg gcgctgttcc
     4621 tgttcgtgct cggcatgctg gcgcagcgcg cgctgccgcg tgccgcgcaa ctgccgcgcg
     4681 ctggcggata cgcgggcgcg ctggtgctgc tcgcgctcat tgcgctgctg ccgcaactgc
     4741 acctgccgga cgagccgtcg cgctggctga tgtactcgtg cgtcgccgcc gtcgctccgc
     4801 tggccttcag cgcgttccgc cacagcgcgt tcgatcgctg gatcggcgat ctcagctatc
     4861 ccatctatct ctgccacctg ctggtggtgg gcttcgtgct c
//
LOCUS       DASZZC010000558         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_742174,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000558 DASZZC010000000
VERSION     DASZZC010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742174"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VHE37_11845"
     CDS             <1..597
                     /locus_tag="VHE37_11845"
                     /inference="COORDINATES: protein motif:HMM:NF018829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HVT36274.1"
                     /translation="FADVADNPGGGGRGNTTELLRALINAGAGGVQLGVMFDPALVRD
                     AHGAGVGANIQAHFNREEPSPLSKPLQAPAQVIALTDGRFRATRGVLAGTQVNLGASC
                     LLRIGGIDVAVISIRQQICSAETLEHFGLDPAQARCLVLKSRGHFRAGFAHLVRDENI
                     FEVDAPGLATPNFASLPWRALPRPLYPLDADMEWAGPP"
     gene            622..>1641
                     /locus_tag="VHE37_11850"
     CDS             622..>1641
                     /locus_tag="VHE37_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HVT36275.1"
                     /translation="MEIPPSSRAPQAPDLPSRALHFTLHFVREFAGWYALILLLQTAA
                     ASAAIAVPWAIGSVIRAVGSGSYDAVRHALTLFIVLGVTELLCTRGAMGTHTYIAPLV
                     RGRATAELFAYIQHHSHRYFTDRFAGALAHRITETAMGVMQTNHAILFVFLPVLVKIG
                     VAATLLGHANFRLALFVLGWAAVFIGIAWALALQCSPYLRAHAAARSSTTGQVIDALS
                     NMTNIRLFAQSGDERKRLGGVLTDELGKTRKAQRYLERIRWFQGTACLALKIGALAGA
                     LWLWRNGAIDAGSFVMCASIALLIIAEADNLGRQFLDFFDYIGNIENGVGTLIRPHEI
                     VDAPGA"
BASE COUNT          242 a          579 c          556 g          264 t
ORIGIN      
        1 ttcgccgatg tcgccgacaa ccccggcggc ggcgggcgcg gcaacaccac cgaactgctg
       61 cgggcgctga tcaacgcggg cgcaggcggc gtgcaactgg gcgtgatgtt cgatcccgcc
      121 ctggtgcgcg atgcgcacgg cgcgggcgtg ggcgcaaaca ttcaggcgca cttcaaccgc
      181 gaggaacctt cgccgctgtc gaagccgctg caggcgccgg cacaggtgat cgcgctcacc
      241 gacggtcgct tccgcgccac gcgcggcgtg ctggccggca cgcaggtgaa tctcggtgca
      301 tcgtgcctgc tgcgcatcgg cggcatcgac gtggcggtca tcagcatccg ccagcagatc
      361 tgctcggcgg agacgctcga acatttcggc ctcgacccgg cgcaggcgcg ctgcctggtg
      421 ctgaaatcgc gcggccattt ccgcgccggc ttcgcccacc tggtgcgcga cgaaaatatt
      481 ttcgaggtgg acgcgccggg cctggccacg cccaatttcg ccagcctgcc gtggcgcgcg
      541 ctgccgcggc cgctgtatcc gctggatgcg gacatggaat gggctgggcc gccctgatcg
      601 cgcacgacta aaattgcggc gatggaaatc ccgccaagtt cccgcgcgcc gcaggcgccc
      661 gacttgccct cgcgcgcgct gcacttcacg ctgcatttcg tgcgcgagtt cgccggctgg
      721 tacgcgctga tcctgctgct gcagaccgcg gcggccagcg ccgccatcgc cgtgccctgg
      781 gccatcggca gcgtgatccg cgcggtcggc agcggcagct acgatgccgt gcggcacgcg
      841 ctgaccctgt tcatcgtgct cggcgtcacc gagctgctgt gcacgcgcgg ggccatgggc
      901 acgcatacct acatcgcgcc gctggtgcgc ggacgcgcga ccgccgaact gttcgcgtac
      961 atccagcacc attcgcaccg ctatttcacc gaccgcttcg ccggcgcgct ggcgcaccgc
     1021 atcaccgaaa cggcgatggg cgtgatgcag accaaccacg ccatcctgtt cgtgttcctg
     1081 ccggtgctgg tgaagatcgg cgtggcggcc acgctgctgg gccacgccaa tttcaggctg
     1141 gcgctgttcg tgctcggctg ggcggcggtc ttcatcggca tcgcctgggc gctggcgctg
     1201 cagtgctcgc catacctgcg cgcgcatgcg gccgcgcgca gcagcaccac cggccaggtg
     1261 atcgatgcgc tgagcaacat gaccaacatc cgcctgttcg cgcagagcgg cgacgagcgc
     1321 aagcgcctgg gcggcgtgct caccgacgaa ctgggcaaga cgcgcaaggc gcagcgttac
     1381 ctcgagcgca tccgctggtt ccagggcacg gcctgcctgg cactgaagat cggcgcgctg
     1441 gccggcgcgc tgtggctgtg gcgcaacggc gccatcgacg ccggcagttt cgtcatgtgc
     1501 gccagcatcg cgctgctgat catcgcggaa gcggacaacc tgggccgcca gttcctggat
     1561 ttcttcgatt acatcggcaa catcgagaac ggcgtaggca cgctgatccg cccgcacgag
     1621 atcgtcgatg cgccgggtgc g
//
LOCUS       DASZZC010000559         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_420116,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000559 DASZZC010000000
VERSION     DASZZC010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420116"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VHE37_11855"
     CDS             complement(<1..300)
                     /locus_tag="VHE37_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HVT36276.1"
                     /translation="MTLQPDWPAPQNVRAAFTLRTADADAAIYARYRTAPGSPQAAKV
                     SANRRRLIRDLKLPSDPAWLQQVHGTRVLRAPWSGEEPQADASYTTQRGVVCVVQS"
     gene            complement(417..1403)
                     /gene="rluD"
                     /locus_tag="VHE37_11860"
     CDS             complement(417..1403)
                     /gene="rluD"
                     /locus_tag="VHE37_11860"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HVT36277.1"
                     /translation="MTSQTPVDGDEELLTAQVPPALAGLRLDQAAAKLFDRYSRARLQ
                     GWIESGALTVNGEVQTRSRTPVADGDELALAAEPEVSHEVLPQDIALEVLHADKSIAV
                     INKPAGLTVHPGAGQRDGTLQNALLFRFPQTAKVPRAGIVHRLDKDTSGVLVVALDLV
                     AHAKLVAAISAREVRREYDALVQGTLIAGATIDAPIGRHPHDRVRMAVVERGGREAVT
                     RYRIAERFAHHTLLNVQLETGRTHQIRVHLAHVRHPIVGDAVYGPSGARVRGAGMPPE
                     LRMVLTDFRRQALHARELELAHPKSDKAMSFAAPRPGDLLQLLDALRKHDPA"
     gene            1450..2277
                     /locus_tag="VHE37_11865"
     CDS             1450..2277
                     /locus_tag="VHE37_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HVT36278.1"
                     /translation="MKKMLIAALALLVAACSSNPDKLPPDNPFKNGPQKNPRELRAEA
                     STLYKSAREALDSGDYSTAITRYDQIAARYPFTDYATQADLERVWALYRSYDPDKALS
                     SADRFLREHPRHPNVDYVQYVKGLINFDRDEGLAGAIGPDTTKEDVANQRRSFDDFAL
                     LVQKYPSSRYAGDARARMIWLRNRLAQHEMHVVRFYVKRGAWIAAAKRAEQVVTQYPG
                     APVTLEAMQIMETSYRKVGLTQQADDAKKLLDAQIAVLPPSEKPRPGFFSRLWPFSA"
     gene            complement(2281..>3344)
                     /locus_tag="VHE37_11870"
     CDS             complement(2281..>3344)
                     /locus_tag="VHE37_11870"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HVT36279.1"
                     /translation="RRRVFDSRVAETGLPIAYVNCVGGQDDVLFDGDSTAVNGDGSIA
                     FAAPLFEEGVFPLQFDGARFSGTQRPPESTEAIVYKGLVQAVRDYVNRNGFPGALIGM
                     SGGIDSALVAAIAADALGKERVWGVSLPSRYTTDMSNDDARIEAERLGIRYSVLPIEP
                     TFQALSGTLAESFAGKKADLTEENLQSRCRGVLLMALSNKFGHVVLTTGNKSEMAVGY
                     ATLYGDMCGGFAPIKDCYKTLVYALSRYRNKLGEVIPERVITRPPSAELRPDQKDSDS
                     LPPYDVLDPILEAYVEQQLSSAEIVRKGFAEADVKRVAALVRRSEYKRRQAPPGPKVT
                     RCAFGRERRYPLTAVYGDV"
BASE COUNT          538 a         1179 c         1103 g          524 t
ORIGIN      
        1 ggactgcacc acgcagacca cgccgcgctg cgtggtgtag ctggcgtcgg cctgcggttc
       61 ttccccggac cagggcgccc gcagcacgcg cgtgccatgc acctgctgca gccacgcggg
      121 atcggacggc agtttcagat cacggatcag ccggcgccgg ttggccgaaa ccttcgccgc
      181 ctgcgggctg ccgggcgccg tgcggtagcg cgcgtagatg gcagcgtcgg cgtcggccgt
      241 gcgcagcgtg aaggccgcgc gcacgttttg cggtgccggc cagtccggct gcagcgtcat
      301 ttctggggtc accgcacgag gcatgttgat gttacaaaaa gatgtatatt tttaatttaa
      361 actgatattt tataacatgc cggcgcgggg tgcggtggtc aggttttcga cctgcttcag
      421 gccggatcat gcttgcgcag cgcgtccagc agctggagca aatcgcccgg gcgtggcgcg
      481 gcgaagctca tcgccttgtc cgatttcgga tgcgccagtt ccagttcgcg cgcatgcagc
      541 gcctggcggc ggaaatccgt cagcaccatg cgcagctccg gcggcatgcc cgcgccgcgc
      601 acgcgcgcgc cgctggggcc gtacaccgcg tcgccgacga tgggatggcg cacatgcgcc
      661 aggtgcacgc ggatctggtg cgtgcgcccg gtctccagct gcacgttgag cagcgtgtga
      721 tgggcgaaac gctcggcgat gcggtagcgc gtcaccgcct cgcgcccgcc gcgctcgacc
      781 accgccatgc gcacgcggtc gtgcggatgg cgcccgatcg gcgcatcgat ggtggcgccg
      841 gcgatcagcg tgccctgcac cagcgcgtcg tactcgcgcc gcacctcgcg cgcggatatc
      901 gccgccacca gtttggcgtg cgccaccagg tccagcgcca ccaccagcac gccggaggtg
      961 tccttgtcga ggcggtgcac gatgccggcg cgcggcactt tcgccgtctg cggaaaacgg
     1021 aacagcagcg cgttctgcag cgtgccgtcg cgctggccgg cgccgggatg cacggtcagg
     1081 ccggccggct tgttgatcac cgcgatcgac ttgtcggcgt gcagcacctc gagcgcgatg
     1141 tcctgcggca gcacctcgtg cgacacctcc ggctcggcgg ccagcgccag ctcgtcgccg
     1201 tccgccaccg gcgtgcgcga gcgcgtctgc acctcgccgt tcaccgtcag cgcgccggat
     1261 tcgatccagc cctgcaaccg cgcgcgcgaa tagcggtcga agagcttggc cgccgcctgg
     1321 tccaggcgca ggccggccag cgccggcggc acctgggccg tcagcagttc ctcgtcgccg
     1381 tccaccgggg tctgggaagt catccgctat actcgccgcc tcgcaaaaag ccgcaccgaa
     1441 agtttccgca tgaagaagat gttgatcgcc gcgctggcgc tgctcgtcgc ggcctgcagt
     1501 tccaaccccg acaagctgcc gccggacaac ccgttcaaga acgggccaca gaagaatccg
     1561 cgcgaactgc gtgccgaagc cagcacattg tataagtcgg cgcgcgaagc gctggactcc
     1621 ggcgactatt ccaccgccat cacgcgctac gaccagatcg ccgcgcgcta cccgttcacc
     1681 gactacgcca cccaggccga cctggagcgc gtgtgggcgc tgtaccgcag ctacgatcct
     1741 gacaaggcgc tgtcgtcggc cgaccgcttc ctgcgcgagc acccgcgcca tcccaacgtc
     1801 gactatgtgc agtacgtgaa gggcctgatc aacttcgacc gcgacgaagg cctggccggc
     1861 gccatcggcc cggacaccac caaggaagac gtcgccaacc agcgccgctc gttcgacgac
     1921 tttgcgctgc tggtgcagaa gtacccaagc tcacgctacg ccggcgatgc gcgcgcgcgc
     1981 atgatctggc tgcgcaaccg cctggcccag cacgagatgc acgtggtgcg cttctacgtc
     2041 aagcgcggcg cgtggatcgc ggcggccaag cgcgccgagc aggtggtcac gcagtacccc
     2101 ggcgcgccgg tcacgctgga agcgatgcag atcatggaaa ccagctaccg caaggtgggc
     2161 ctgacccagc aggccgacga cgcgaagaag ctgctcgacg cgcagatcgc cgtgctgccg
     2221 cccagcgaga aacccaggcc cgggttcttc tcgcgcctgt ggccgttcag cgcctgacct
     2281 tcatacgtcg ccgtagaccg cggtcagcgg gtagcggcgt tcgcgcccga acgcgcagcg
     2341 cgtgaccttg gggcccggcg gcgcctgccg gcgcttgtac tccgagcggc gcaccagcgc
     2401 agccacgcgc ttcacgtccg cctcggcaaa acccttgcgc acgatctcgg cactcgacaa
     2461 ctgctgctcg acgtatgcct cgaggatcgg gtcgagcacg tcgtacggcg gcagcgaatc
     2521 ggaatccttc tggtccggcc gcagttcggc cgatggtggc cgcgtgatca cccgctcggg
     2581 gatcacctcg cccagcttgt tgcggtaacg cgacagcgcg tagaccagcg tcttgtagca
     2641 atccttgatc ggcgcgaagc caccgcacat gtcgccgtag agcgtggcgt agcccaccgc
     2701 catctcgctc ttgttgccgg tggtgagcac cacgtggccg aacttgttgg acaaggccat
     2761 cagcagcacg ccgcggcagc gcgactgcag gttttcctcg gtgaggtcgg cctttttccc
     2821 ggcgaagctt tccgccagcg tgccgctcaa ggcctggaat gtcggctcga tcggcagcac
     2881 cgaatacctg atgcccaggc gctcggcctc gatgcgcgca tcgtcgttgg acatgtccgt
     2941 ggtgtagcgc gacggcagcg acacgcccca cacgcgttcc ttgccgagcg cgtcggcggc
     3001 gatggcggcg accagcgccg aatcgatgcc gcccgacatg ccgatcagcg cgccgggaaa
     3061 gccgttgcgg ttgacgtagt cgcgcaccgc ctgcaccagg cccttgtaga cgatggcctc
     3121 ggtgctctcg ggcgggcgct gcgtgccgga aaaacgcgcg ccgtcgaact gcagcggaaa
     3181 cacgccttcc tcgaacagcg gcgcggcgaa ggcgatggag ccatcgccat tgaccgcggt
     3241 ggaatcgcca tcgaacagca cgtcgtcctg gccgcccacg cagttcacgt aggcgatcgg
     3301 cagaccggtc tcggccacgc gcgaatcgaa cacgcggcgg cgct
//
LOCUS       DASZZC010000560         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1261381,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000560 DASZZC010000000
VERSION     DASZZC010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261381"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VHE37_11875"
     CDS             <1..958
                     /locus_tag="VHE37_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36280.1"
                     /translation="VPGLGTTLDQGAIFFGHALCMTDDGMTAYATSSAQSNAVIDLAD
                     ITSPKLIQLFAPAAHDCGVSPDGTRLYLAVFGFESLGLGIPNGPSVGQNGLMILDATA
                     IQNRTVATAPLLFGTAPPKVGFLGWSNVADGEAPSAGSHTARWFRNHGRSYVYSSDEW
                     PTAGVCPWSHSRIIDVTDEANPVKVADIRLEVDDIANCAKTEIDNANYSAHYVGIDNM
                     NEATTLFTTNYSAGLRVWDIHDPAHPKEIAYWHPAPNPNTPAVPFAAEFGSSGSLWDA
                     VPTYVRYRPESGQIWIASYSAGFQILEFTASAGPTAPRPTAR"
     gene            complement(1098..1367)
                     /locus_tag="VHE37_11880"
     CDS             complement(1098..1367)
                     /locus_tag="VHE37_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HVT36281.1"
                     /translation="MNCRPGCGACCIAPSISSPIPGMPQGKPAGVRCIHLTDDYRCGI
                     FHDPRRPRVCASLQAEPVMCGETREHALVYLADLQRRTEPLQVSK"
     gene            complement(1416..2270)
                     /locus_tag="VHE37_11885"
     CDS             complement(1416..2270)
                     /locus_tag="VHE37_11885"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HVT36282.1"
                     /translation="MPCEPSALKEVELFQHLDDDDCRQLAQMIDLRPLAAGTTLFHAG
                     EPGESLYIVNRGEIELSIRDTTGQKISLAVVGAGQVFGELALLDRGPRSATAVSLIES
                     ELLELRRDELELLFRKSPGAALSLLAAMGHMTRKADELLKTRVARNVNEEVAESLSTF
                     QRIADWISEFSGSMSFLILNGLWFLVWIAINTLPLGVEQFDPFPFGLLTMIVSLEAIF
                     LSCFVLISQNRQAEKDRVRSDIEYEVNIKAELEVAHLHEKADRIHAEMLERFARIEKR
                     LAEAARTH"
     gene            complement(2395..>3771)
                     /locus_tag="VHE37_11890"
     CDS             complement(2395..>3771)
                     /locus_tag="VHE37_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36283.1"
                     /translation="VVPDGYRRVRASNFNTATNSIIDSATFDGDPVVDATVTVQKVAP
                     GTQACDSNIQIVIQTMIPNTITTAERQWVSTACAIKLDYISNIGGATGTIVSATLFNS
                     HKNKSVTLTGGQFRMYHHHGVAGDMPAQLPDGIFNLLQPTGDGTFELPPDQYFAIDNN
                     PAPPSSGFNYGVSPDDGTPPFNGNASDRIVWGIQNASFNGPGSYDCAPNNKLSMFVEV
                     GQYSNELVYESDNINSDNDGSCTVTITKQSGRLYAAHYTATLVGIDGTPALLSTAQRT
                     LHLAGRFRNFIIVPIAAGNQGNEGNLPDTGAATTLHIDDGNTVFTKGQQFVLGASSGA
                     VPATGAYSLVFQRTLPFNTAPNDKIRKSVSVNLTQVPHGTGTFNCGDTGSFFAIAVRT
                     AQGIDYGYASNVAGTSCSVTIDSFDAQTVTGHYSAVLRNADAEAFLPGGDATITVSGQ
                     FRQAVQQQ"
BASE COUNT          624 a         1210 c         1268 g          669 t
ORIGIN      
        1 ggtgccgggg ctgggcacca cgctcgacca gggcgcgatt ttcttcggcc acgcgctgtg
       61 catgaccgac gacggcatga cggcgtacgc caccagttcc gcgcagagca acgcggtgat
      121 cgacctggcc gacatcacca gtcccaaact catccagctg ttcgcccccg ccgcgcacga
      181 ctgcggcgtc agcccggacg gcacgcggtt gtacctcgcg gtgttcggtt tcgaatcgct
      241 gggcctgggc atacccaacg ggccgtcggt gggacagaac gggctgatga tcctcgacgc
      301 cacggcgatc cagaaccgca cggtcgccac cgcgccgctg ctgttcggca ccgcgccgcc
      361 gaaggtggga tttcttggct ggagcaacgt ggccgacggc gaagcgccga gcgcgggttc
      421 gcacaccgcg cgctggttcc gcaaccacgg gcgcagctac gtgtattcga gcgacgagtg
      481 gccaaccgcc ggcgtgtgcc cgtggtcgca cagccgcatc atcgacgtga ccgacgaggc
      541 caacccggtg aaggtggccg acatccgcct cgaagtcgat gacatcgcca attgcgcgaa
      601 gaccgagatc gacaatgcca attactccgc gcattacgtc ggcatcgaca acatgaacga
      661 ggcgaccacg ctgttcacca ccaactacag cgcggggcta cgcgtgtggg acatccacga
      721 cccggcgcat ccgaaggaga tcgcctactg gcacccggcg cccaacccga acacgccggc
      781 ggtgccgttc gccgcggagt tcggcagcag cggctcgctg tgggacgcgg tgccgaccta
      841 cgtgcgctac cggccggaga gcgggcagat ctggattgcc agctacagcg cgggcttcca
      901 gatcctggag ttcacggcga gcgcgggacc aaccgcgccg aggcccaccg cgcgctgagg
      961 cgcaaaaaat gcccgcgcgg acgtccaccg cacatgcctt ttgcgtgtta caaaatattt
     1021 actgaattta taattatata ataattaaaa acacgctgag gccgggtgcg gtggactgat
     1081 tttttgcctc cgcgcgctca cttcgacacc tgcagcggct ccgtgcgccg ctgcaggtcg
     1141 gccaagtaca ccagcgcatg ctcgcgcgtc tcgccgcaca tgacgggctc cgcctgcagg
     1201 ctggcgcaca cgcgcgggcg ccgcgggtcg tggaagatgc cgcaacgata gtcatcggtt
     1261 agatggatgc aacgcacgcc ggcaggcttg ccctgcggca tgccgggaat cggcgaggag
     1321 atcgagggcg cgatacagca ggcgccacag ccaggtcggc agttcatgtg cgcattgtca
     1381 gcgagcgcgg cgtcgcgtgc gagcaggaaa tttcgtcagt gcgtccgcgc cgcctcggcc
     1441 agcctcttct cgatgcgcgc aaagcgctcc agcatctcgg catggatgcg gtcggccttc
     1501 tcgtgcaggt gcgccacttc cagctcggcc ttgatgttca cctcgtactc gatgtcggag
     1561 cgcacacggt ccttctccgc ctggcggttc tggctgatca gcacgaagca cgacaggaag
     1621 attgcttcga gcgacacgat catggtgagc aggccgaagg gaaatggatc gaactgctcg
     1681 acgcccagcg gaagcgtgtt aatggcaatc cacaccagga accacagccc gttgaggatc
     1741 aggaagctca tgctgccgct gaactcggag atccagtcgg cgatgcgctg gaacgtgctg
     1801 aggctttccg ccacttcctc gttgacgttg cgcgccacgc gcgtcttcag cagctcgtcc
     1861 gccttgcgcg tcatgtggcc catggcggcg agcagcgaca gcgccgcacc ggggctcttg
     1921 cggaacagca gctccagctc gtcgcggcgc agctccagca gttccgattc gatcaatgac
     1981 accgcggtgg cagagcgcgg tccgcggtcc agcagcgcca gttcgccgaa cacctggccg
     2041 gcgccgacca cggccagcga gatcttctgc ccggtggtgt cgcggatcga cagttcgatc
     2101 tcgccgcggt tgacgatgta cagcgactcg cccggctcgc cggcgtggaa cagcgtggtg
     2161 ccggcagcca gcggtcgcag gtcgatcatc tgcgccagct ggcggcagtc gtcatcgtcc
     2221 aggtgctgga acagttcgac ttctttcagc gcggacggct cgcacggcat tgcatcgcac
     2281 tccggcaaga caggtgcggc aagcatatcg ggatcgccgc gccggcggca gcggctttac
     2341 gcggcccaaa aaaacgggcg ccgcgcgggc gcccgtcgat gaccccgcgc atttctactg
     2401 ctgctgcacc gcctggcgga actggcccga cacggtgatc gtcgcgtcgc cgcctggcag
     2461 gaacgcctcg gcgtcggcat tgcgcagcac cgcgctgtag tggcccgtca ccgtctgcgc
     2521 atcgaagctg tcgatggtga cgctgcagct ggtgccggca acattgctgg catagccgta
     2581 gtcaatgccc tgcgccgtgc gcacggcgat ggcgaagaag ctgccggtgt cgccgcagtt
     2641 gaaggtgccg gtgccgtgcg gcacctgcgt caggttcacc gacacgcttt tacggatctt
     2701 gtcgttgggc gccgtgttga acggcagcgt gcgctggaac accagcgagt acgcaccggt
     2761 ggccggcacc gcgccgctgc tggcgccgag cacgaactgc tgccccttgg taaataccgt
     2821 gttgccgtcg tcgatgtgca gcgtggtggc ggcaccggta tcgggcaggt tgccctcgtt
     2881 gccctggttg ccggcagcaa tcggcacgat gatgaaattg cggaagcggc cggccaggtg
     2941 cagcgtgcgc tgcgccgtgc tcagcagcgc gggcgtgccg tcgatgccga ccagcgtcgc
     3001 cgtgtaatgc gccgcgtaca ggcggccgct ctgcttggtg atggtcaccg tgcagctgcc
     3061 gtcgttgtcg ctgttgatgt tgtcgctctc gtacaccagt tcgttgctgt actggccgac
     3121 ctcgacgaac atcgacagct tgttgttggg cgcgcagtcg tagctgcccg gcccgttgaa
     3181 cgacgcgttc tggatgcccc acacgatgcg gtctgacgcg ttgccgttga acggcggcgt
     3241 gccgtcatcc ggcgagacgc cgtagttgaa gccggatgac ggcggcgccg ggttgttgtc
     3301 aatggcgaag tactggtccg gcggcagttc gaaggtgccg tcgccggtgg gctgcagcag
     3361 gttgaagatg ccgtcgggca gctgggccgg catgtcgccg gccacgccgt ggtggtgata
     3421 catgcggaac tggccgccgg tcagtgtcac cgacttgttc ttgtggctgt tgaacagcgt
     3481 ggcgctgacg atggtgccgg tggcgccgcc gatgttgctg atgtaatcca gcttgatcgc
     3541 gcaggcggtg gacacccact gccgctcggc ggtggtgatg gtgttgggaa tcattgtctg
     3601 gatgacgatc tggatgttgc tgtcgcaggc ctgcgtgccc ggcgccactt tctgcacggt
     3661 gacggtggca tcgaccaccg ggtcgccgtc gaaggtcgcg ctgtcgatga tgctgttggt
     3721 cgcggtgttg aagttgctgg cccgcacgcg gcggtagcca tcgggcacca c
//
LOCUS       DASZZC010000561         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_368940,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000561 DASZZC010000000
VERSION     DASZZC010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368940"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VHE37_11895"
     CDS             <1..498
                     /locus_tag="VHE37_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36284.1"
                     /translation="LGRIAYAGMQWTEAVAHLRSAVQLAPRNAGYHNQLGDALAKYID
                     DVTVFRKPTVARQLRDEYIRAIELDPEMLDARESLLQFYVRAPGFVGGDLDKAREQAR
                     EIARRDAARGYKAYGDVDAADDRFDAAAREYRQALQLNPKYADAREALDKLGERGSSR
                     KPTHI"
     gene            878..>2508
                     /gene="leuA"
                     /locus_tag="VHE37_11900"
     CDS             878..>2508
                     /gene="leuA"
                     /locus_tag="VHE37_11900"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HVT36285.1"
                     /translation="MPMLNKPSDKYRAFAPVKLRDRNWPDAVLTQAPLWLSTDLRDGN
                     QALIEPMNRARKLRMFEQLVKIGFKEIEIGFPSASQTEFDFLRHLIDNQLIPDDVWVQ
                     VLTPAREPLIRRTFEALKGAPRAIVHYYNATAPVMRRVVFNMSQDQVLDELAVAHAKL
                     IKQLAAERPQTQWRFEYSPEMFSGTELEFSKRVCDAVSEVFQPTPQNKLIINLPSTVE
                     HSTPNIYADMIEWMHRNLARRDSILISLHPHNDRGTGTAAAELGMMAGADRVEGCLFG
                     NGERTGNVDIVNLALNLYTQGVHPNLDFSSIDDIRQCVEYCNQLPVHPRHPYVGDLVY
                     TSFSGSHQDAIKKAFSARKEGDIWEMPYLPIDPMDLGRSYEAVIRINSQSGKGGIAYL
                     LEAEYGLVLPRRMQIEFSAVVQEVADVSGKELTAEDLWSVFSNEYLETTQPYGYIAHH
                     LVEDSRSAVDLTTEISSDGKRMKLAGQGNGPIDAFVAGLQKLTGQKLTVLDYHEHAIG
                     AGADAKAITYIELRVGEGKPLHGVGLDSNIVTASL"
BASE COUNT          499 a          828 c          774 g          407 t
ORIGIN      
        1 ctcgggcgca tcgcctacgc cggcatgcaa tggaccgagg ccgtggccca tctgcgcagc
       61 gcggtgcagc tggcgccgcg caatgccggg tatcacaacc agctgggcga tgcactggcg
      121 aagtacatcg atgacgtcac cgtgttccgc aagccgacgg tggcgcgcca gctgcgagac
      181 gaatacatcc gtgccattga gctcgacccg gaaatgctcg acgcgcgcga atcgctgctg
      241 cagttctacg tgcgcgcgcc gggattcgtc ggcggcgacc tggacaaggc gcgcgagcag
      301 gcgcgcgaaa tcgcccggcg cgatgctgcg cgcggctaca aggcctatgg cgacgtggat
      361 gcagcggatg accgcttcga tgccgccgca cgcgaatacc ggcaggcgct gcagctcaat
      421 ccgaagtacg ccgatgcgcg tgaggcgctc gacaagctgg gcgagcgcgg ctcgagccgg
      481 aagcccacgc atatctgaaa agcggacgct gcaggtccca ccgcatcccc attatccatg
      541 ttattaaata tatattgaat aataaaatat atgataatta ataacataca aaaccggcat
      601 gcggtcgagc cagaaatgcc gtctgtcggt gttcgcgttg cccgcggcgc gcggcttggt
      661 ctatagtccg ccggccatga attccgtcca cccgcacgca gcgatgtccc cggcgccccg
      721 cgccgcgctg ctgcgcgcgc tggacctgct tctgctgctt ctgccgtaag gcagaacgaa
      781 cgcacacggc cgcacggggc ctcaagccgt gccaacccaa gcagaacttc actcccgccg
      841 gaaccgcggg atcaatccga attgatggag ctgtgccatg ccgatgttga acaagccgtc
      901 tgacaagtac cgtgcctttg cgccggtgaa gctgcgcgac cgcaactggc ccgatgccgt
      961 gctgacccag gcgccgctgt ggctgtccac cgatctgcgc gacggcaacc aggcgctgat
     1021 cgaaccgatg aaccgcgcgc gcaagctgcg catgttcgag caactggtga agatcggttt
     1081 caaggagatc gagatcggct tcccctcggc ctcgcagacc gagttcgatt tcctgcgcca
     1141 cctgatcgac aaccagctga tccccgatga cgtatgggtg caggtgctca cgcctgcgcg
     1201 cgagccgttg atccgccgca ccttcgaagc gctgaagggc gcaccgcgcg ccatcgtgca
     1261 ctactacaac gccaccgcgc cggtgatgcg ccgcgtggtg ttcaacatga gccaggacca
     1321 ggtcctcgac gaactggcgg tggcgcatgc gaaactgatc aagcagctgg ccgccgagcg
     1381 tccgcagacg caatggcgct ttgaatattc gcccgagatg ttttccggca ccgagctgga
     1441 attttccaag cgcgtctgcg atgcggtgag cgaggtgttc cagccgacgc cgcagaacaa
     1501 gctgatcatc aaccttccgt cgacggtgga gcattccacg ccgaacatct acgccgacat
     1561 gattgagtgg atgcaccgga atctggcgcg gcgcgacagc atcctcatct cgctgcaccc
     1621 gcataacgac cgcggtaccg gcaccgccgc cgccgaactg ggcatgatgg ccggcgccga
     1681 ccgcgtcgaa ggctgcctgt tcggcaacgg cgagcgcacc ggcaatgtcg acatcgtcaa
     1741 cctcgcgctc aacctgtaca cgcagggcgt ccatccgaac ctggacttct ccagcatcga
     1801 cgacatccgc cagtgcgtgg agtactgcaa ccagctgccg gtgcatccgc gccatccgta
     1861 cgtcggcgac ctggtctaca ccagcttctc cggctcgcac caggacgcca tcaagaaggc
     1921 gttttccgcg cgcaaggagg gcgacatctg ggaaatgccc tacctgccga tcgacccgat
     1981 ggacctgggc cgcagctacg aggcggtgat ccgcatcaat tcgcagtcgg gcaagggcgg
     2041 catcgcctac ctgctcgaag ccgaatacgg cctggtgctg ccgcggcgca tgcagatcga
     2101 attctccgcg gtggtgcagg aagtggctga cgtctccggc aaggagctaa ccgccgagga
     2161 cctgtggagc gtgttcagca atgaatacct ggagacgacg cagccgtacg gctacatcgc
     2221 gcaccacctg gtggaagatt cacgcagcgc ggtggacctg accaccgaga tcagcagcga
     2281 cggcaagcgc atgaagctgg ccgggcaggg caacgggccc attgacgctt ttgtggccgg
     2341 cctgcagaag ctcaccgggc agaagctgac cgtgctggat taccacgagc acgccattgg
     2401 cgccggcgcc gacgccaagg ccatcaccta catcgagctg cgcgtcggcg agggcaagcc
     2461 gctgcacggc gtgggcctgg attccaacat cgtcaccgcc tcgctcaa
//
LOCUS       DASZZC010000562         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_951798,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000562 DASZZC010000000
VERSION     DASZZC010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951798"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1330
                     /locus_tag="VHE37_11905"
     CDS             173..1330
                     /locus_tag="VHE37_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36286.1"
                     /translation="MVGGIPIEFLLFALTLVGVAVLHDHALWVALAGLGAVLGWKLAT
                     QAGFAVAAHLAHEAPELLNLFGLLLGFALLARHFERSEIPDLLPRLLPRGWPGNFALL
                     GAVFVMSAFLDNIAAAMIGGMVARHVYGGRVDIGFVAAIVAASNAGGAGSVLGDTTTT
                     MLWIAGVSWRDVLYAYLAAVPALLFFGIAASLQQERTQAMTHELKAGARLRLPHLVAT
                     VLVLLGALAANVMFDFPAAGVWTAILICSSFATTDWLELREASRGAVFLLALVACASL
                     MPVERLPPASMQTTLALGLVSAVFDNIPLTKLALEQGGYDWGALAYCVGFGGSMIWFG
                     SSAGVAISGEFPQSKSVLAWVARGWHVIAGYFIGYASLMLLSGWQPHLLAR"
     gene            1484..1912
                     /gene="ndhC"
                     /locus_tag="VHE37_11910"
     CDS             1484..1912
                     /gene="ndhC"
                     /locus_tag="VHE37_11910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVT36287.1"
                     /translation="MDFIAHMPPASWSLLVYAAGVVGLCLLMLGLSWVLGGRDWGRAK
                     HEPFESGVVGAGDAHMRLSAKFYLVAMFFVIFDVEAVFLYAWAISLKQAGWAGFIEAT
                     IFIFILAASLVYLWRVGALEWAPESRKRFKLKHPDQPGQS"
     gene            1925..2593
                     /locus_tag="VHE37_11915"
     CDS             1925..2593
                     /locus_tag="VHE37_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HVT36288.1"
                     /translation="MEYTLTRIDQNSQRYPMQQREQVADPLDTQIHKNVYMGKLQEML
                     HGAVNWGRKNSIWPYNFGLSCCYVEMATAFTPTHDLARFGAEVIRASPRQADLMVIAG
                     TCFIKMAPVVQRLYEQMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYIPGC
                     PPRPEAFLQGLTLLQESIGKERRPLSWVVGDQGVYKANLPAQREVKRPERSATDKLRS
                     PDSV"
     gene            2600..4378
                     /gene="nuoC"
                     /locus_tag="VHE37_11920"
     CDS             2600..4378
                     /gene="nuoC"
                     /locus_tag="VHE37_11920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HVT36289.1"
                     /translation="MAADSPPMLPDVVRDLYARFGESLFVAQQARDGMPTLWVPADRI
                     IEVLKYLKNEIAKPYRMLYDLCGVDERVRKYRLDLPPADFTVVYQLLSIERATPSSCG
                     DLRLKVALLGDKPSIPSCVGIYRNANWYERETWDMFGIVFAGHPNLRRILLPPRWEGH
                     PLRKEYHARATEFDPFMLDQMRQDLDQEALRFVPEEWGMKRGTAEEDFMFLNLGPNHP
                     SAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHTYIPYTDRIDYLGGVMN
                     NLPYVLTVEKLAGIEVPDRVKVIRIMMAEFFRITSHLLFYGTFCQDVGAMSPVFFMFV
                     DRAKAYDVIEAITGFRMHPAWYRIGGVAHDLPNGWEQKVREFVEWLPRRLDEYQKAAL
                     ENGILKVRSKGVAAYRTADALEWGVTGPGLRATGCGFDLRKARPYSGYENFEFEVPVA
                     DGGDVYDRCVVRVEEMRQSIRIIKQCLENMPAGPYKADHPLTCPPPKERTLKDIETLI
                     THFLGVSWGPVIPAGEAATSIEATKGINSYYLTSDGSTMSYRTRIRTPSFAHLQQIPA
                     AINGEMIPDLIAYIASIDFVMADVDR"
     gene            4375..>4640
                     /locus_tag="VHE37_11925"
     CDS             4375..>4640
                     /locus_tag="VHE37_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HVT36290.1"
                     /translation="MSAGDVVKLADLPRQLEFKLTDQERREIEHELTHYPYGRAASIE
                     ALKIVQKHRGWVPDAAIPEIAKVIGIPAAELDGVATFYSLIFRQP"
BASE COUNT          751 a         1485 c         1559 g          845 t
ORIGIN      
        1 cgccgacggc gtggtcatcg gccgcgtgtc gatgccgctg gtgcatgagg gcgacgccgt
       61 gttccacatc gcgcgtttcg acgacagcag cgcagcggcc ggcgtggcgc ggcgcatccg
      121 caaggcacgc gcctcgcgcc gcgggcgccg ccgcaagccg cacggctgac tcgtggtcgg
      181 cggaattccg atcgagttcc tgctgttcgc gctcacgttg gtcggcgtgg cggtgctgca
      241 tgaccatgcc ctgtgggtgg cgctggccgg gctgggcgca gtgctcggct ggaagctggc
      301 gacgcaggct ggcttcgccg tcgcagccca cctggcgcat gaagcgccgg aactgctcaa
      361 cctgttcggc ctgctgctgg gcttcgcgct gctggcgcgg catttcgaac gctcggaaat
      421 tcccgacctg ctgccgcggc tcctgccgcg cggctggccg ggcaacttcg ccctgctggg
      481 ggcagtgttc gtgatgtcgg cattcctcga caacatcgcc gccgccatga tcggcggcat
      541 ggtggcgcgc cacgtgtatg gcggccgcgt cgacatcggc tttgtcgccg ccatcgtggc
      601 cgccagcaac gccggcggtg ccggcagcgt gctgggcgac accaccacga ccatgctatg
      661 gattgccggg gtgagctggc gcgacgtgct gtacgcctac ctcgccgcag tgccggcgct
      721 gctgttcttc ggcatcgccg cctcgctgca gcaggagcgc acacaggcga tgacgcacga
      781 gctgaaagcc ggagcgcggc tgcggttgcc gcacctggtt gcgacggtgc tggtgctgct
      841 cggtgcgctg gccgccaacg tgatgttcga ttttcccgct gccggcgtct ggactgcgat
      901 cctgatctgc tcttcgttcg ccacgaccga ttggcttgaa ttgcgcgaag ccagccgcgg
      961 cgccgttttc ttgctggcgc tggtggcgtg cgcttcgctg atgccggtgg agcggctgcc
     1021 gcccgccagc atgcagacga cgctggcact tggactggtg tccgcggtgt tcgacaatat
     1081 tccgctgacc aagcttgcgc tggagcaggg cggttacgac tggggggcgc tggcctattg
     1141 cgtgggcttc ggcggttcga tgatctggtt cggctcgtcg gccggcgtgg cgatctccgg
     1201 cgagtttccg cagtccaagt ccgtgctggc ctgggtggcg cgcggctggc acgtgatcgc
     1261 cggttacttc atcggctatg ccagcctgat gctgctgtcg ggttggcagc cgcacctgct
     1321 ggcgcgctga acctcgcaag aaagttggca aacgcattca aaaagcgcgg cgtgctggcg
     1381 tatcatattg tttgtccgcg caccccatcc gtttgacgtc agccggcgcg cgggaaaatt
     1441 cgaataaacc gggagcccac gggcctaggg acgaggatca ggcatggatt tcatcgcgca
     1501 tatgccgccc gcgtcatggt cgctgctggt ctacgcagca ggcgtggtcg gcctgtgcct
     1561 gctgatgctc gggttgtcct gggtgttggg tggccgcgac tggggccgcg ccaagcacga
     1621 gccgttcgaa tcgggcgtgg tcggtgccgg cgacgcgcac atgcgcttgt cggccaagtt
     1681 ctacctggtg gccatgttct tcgtgatctt cgacgtcgaa gcggtgttcc tctatgcctg
     1741 ggccatttcg ctcaagcagg ccggctgggc cgggttcatc gaggccacca tcttcatctt
     1801 catcctggcg gcgagcctgg tctacctgtg gcgcgtcggc gcgctggagt gggcaccgga
     1861 atcgcgcaag cgcttcaagc tgaagcaccc cgaccagccg ggccagagct gaggcgcaag
     1921 caccatggaa tacaccctga cccgcatcga ccagaactcg cagcgctacc cgatgcagca
     1981 gcgcgagcag gtggctgacc cgctcgacac gcagatccac aagaacgtgt acatgggcaa
     2041 gctgcaggaa atgctgcacg gcgcggtcaa ctggggccgc aagaactcga tctggccgta
     2101 caacttcggc ctgagctgct gctacgtgga gatggccacc gcgttcacgc cgacgcatga
     2161 cctcgcgcgc ttcggcgcgg aagtgatccg cgcgtctccg cgccaggccg acctgatggt
     2221 catcgccggc acctgcttca tcaagatggc gccggtggtg cagcgtctgt acgagcagat
     2281 gctggaaccc aagtgggtca tcagcatggg gtcgtgtgcc aattccggcg gcatgtacga
     2341 catctattcg gtggtgcagg gcgtggacaa gttcctgccg gtggatgtgt acattcccgg
     2401 ctgcccgccg cgcccggagg cgttcctgca agggctgacc ctgctgcagg aatcgatcgg
     2461 caaggaacgc cgcccgctgt catgggtggt cggcgaccag ggcgtgtaca aggccaacct
     2521 gccggcgcag cgcgaagtaa aacgtcccga gcgcagcgcc accgacaagc tgcgcagccc
     2581 ggattccgtc tgattctgca tggccgccga tagccctccc atgctgcccg acgtcgtgcg
     2641 cgacctgtac gcgcgcttcg gcgagtcgtt gttcgtcgcg cagcaggcgc gcgatggcat
     2701 gccgacgctg tgggtgccgg ccgatcgcat catcgaggtg ctcaagtacc tgaagaacga
     2761 gatcgccaag ccgtaccgca tgctgtacga cctgtgcggc gtcgacgagc gcgtgcgcaa
     2821 gtaccggctg gacctgccgc cggccgactt cacggtggtg taccagctgt tgtcgatcga
     2881 acgcgctacg ccgtcgtcct gcggggatct tcgcctgaaa gtggcgctcc tcggcgacaa
     2941 gccttccatc ccgagctgcg tcggcatcta ccggaatgcc aactggtacg agcgcgaaac
     3001 ctgggacatg ttcggcatcg tcttcgccgg ccatcccaac ctgcggcgca tcctgctgcc
     3061 gccgcgctgg gaaggtcatc cgctgcgcaa ggagtaccac gcgcgggcga cggagttcga
     3121 tccgttcatg ctcgaccaga tgcgccagga cctggaccag gaagcgctgc gcttcgtgcc
     3181 ggaagaatgg ggcatgaagc gtggcaccgc ggaagaggat ttcatgttcc tcaacctcgg
     3241 gccgaatcat ccgtcggcgc acggcgcgtt ccgcatcgtg ctgcagctcg atggcgagga
     3301 gatcgtggat tgcgtgccgg acatcggcta ccaccaccgc ggcgccgaga agatgggcga
     3361 gcgccagagc tggcacacct atattccgta caccgaccgc atcgactatc tcggcggcgt
     3421 gatgaacaac ctgccgtacg tgctgacggt ggagaagctg gccggcatcg aggtgccgga
     3481 ccgggtcaag gtgatccgca tcatgatggc ggagttcttc cgcatcacct cgcacctgtt
     3541 gttctacggc accttctgcc aggacgtcgg cgccatgagc ccggtgttct tcatgttcgt
     3601 cgaccgtgcc aaggcctacg acgtgatcga ggccattacc ggtttccgca tgcatccggc
     3661 ctggtatcgc atcgggggcg tggcgcatga cctgcccaac ggctgggaac agaaagtgcg
     3721 cgagttcgtc gagtggctgc ccaggcgcct cgatgaatat cagaaggcgg cactggaaaa
     3781 cggcatcctg aaagtgcgct cgaaaggtgt agccgcctat cgcacggctg atgcgctgga
     3841 gtggggcgtt accggtcccg ggctgcgcgc taccggctgc ggcttcgacc tgcgcaaagc
     3901 gcggccctat tcgggctacg agaacttcga gttcgaagtg ccggttgccg atggcggcga
     3961 tgtgtacgac cgttgcgtgg tgcgcgtcga ggagatgcgc cagtccatcc gcatcatcaa
     4021 gcagtgcctg gagaacatgc cggcgggtcc gtacaaggcg gaccatccgc tgacctgtcc
     4081 gccgccgaag gagcgcacgc tcaaggatat cgagacgctg atcactcact tcctcggcgt
     4141 gtcgtggggc ccggtgattc cggcgggcga ggcggccacc tcgatcgagg cgaccaaggg
     4201 catcaacagc tattacctga ccagcgacgg cagcaccatg agctatcgca cgcgcatccg
     4261 cacgccgtcg ttcgcgcacc tgcagcagat tcctgcggcg atcaacggcg agatgatccc
     4321 ggacctgatc gcctacatcg cttcgatcga tttcgtcatg gcggacgtgg accgatgagc
     4381 gccggcgatg tggtgaagct ggccgacctg cccaggcagc tggagttcaa gctgacggac
     4441 caggaacggc gcgagatcga gcacgagctc acgcactacc cttacgggcg cgcggcaagc
     4501 atcgaggcgc tgaagatcgt gcagaagcac cgcggctggg tgcccgacgc cgccattccc
     4561 gaaatcgcca aggtcatcgg catccctgcg gccgagctcg atggtgtggc gaccttctac
     4621 agcctgatct tccgccagcc
//
LOCUS       DASZZC010000563         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_812197,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000563 DASZZC010000000
VERSION     DASZZC010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812197"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VHE37_11930"
     CDS             <1..1361
                     /locus_tag="VHE37_11930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HVT36291.1"
                     /translation="HVGALMTFKLPSDAPPDFVRKLMLQMREKPFMPEPFGYRLARGR
                     LARVAPAWEETTVDMEYHMRHSALPYPGGERELGVLVARMHSHPLDLSRPLWECHLIE
                     GLEGNRFALYFKAHHCAIDGMGAMKMVQKWLSPDPDDRSGIVGPWMVAARPPPETGDG
                     EDRWARFRKPVRAAAEQARGLKELVRVIAKMTRGADSGTRASLATPRSMFNVNISQQR
                     RLGTQILDLARIKAIGEKTASSANDVLLAVCAGAIRRYLIEQKALPEKSLLASVPVGL
                     PRPDGKSGNAVAGFVVPLGTDITDPAERLAQINRVTTRSKQELMELPQAGLMQFALLG
                     LAPLLLSQVTGLGARMPPIFNFTMSNVVLSKHPLYLMGAELEAMYPVSFLLDGYGLNI
                     TLVGYRDRVAIGVLGCRDAIPSLQRLAIYTTDALAELEEAVGVVPRKPVKLKRRKPAP
                     AA"
     gene            1383..>1775
                     /locus_tag="VHE37_11935"
     CDS             1383..>1775
                     /locus_tag="VHE37_11935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HVT36292.1"
                     /translation="MHIRAFTLADEACVIDLWRRCDLLRPWNDPHKDIRRKLTTQPEL
                     FLVGEIDGQLVASVMAGFDGHRGWVNYLAVAPDQRKAGLGRTLMRHVESELEARGCPK
                     LNLQVRASNQAVLEFYRRIGYGTDDVVSL"
BASE COUNT          296 a          623 c          598 g          258 t
ORIGIN      
        1 tgcacgtggg cgccttgatg accttcaagc tgccgtccga tgcgccgccg gatttcgtgc
       61 gcaagctgat gctgcagatg cgcgaaaagc ccttcatgcc cgaaccgttc ggctaccggc
      121 tggcgcgcgg ccggctggcg cgcgtggccc cggcctggga agaaaccacg gtggacatgg
      181 aataccacat gcgccactcg gcactgccct atcccggcgg cgaacgcgag ctgggcgtgc
      241 tggtggcgcg catgcacagc catccgctgg acctgtcgcg gccgctgtgg gaatgccacc
      301 tgatcgaagg actcgaaggc aaccgcttcg ccctgtactt caaggcgcac cattgcgcca
      361 tcgacggcat gggcgcgatg aagatggtgc agaaatggct gtcgcccgat cccgacgacc
      421 gctccggcat cgtcgggcca tggatggtgg cggcacggcc accgccggaa accggcgacg
      481 gcgaagacag gtgggcgcgc ttccgcaagc cggtgcgcgc cgccgccgag caggcgcgcg
      541 gcctcaagga actggtgcgc gtgatcgcca agatgacgcg cggcgccgac agcggcacgc
      601 gcgcatcgct ggccacgccg cgcagcatgt tcaacgtcaa catcagccag cagcgccgac
      661 tgggcacgca gatcctcgac ctcgcgcgca tcaaggccat cggcgagaag accgcgtcat
      721 cggccaacga cgtgctgctg gcggtgtgcg ccggcgccat ccgccgctac ctgatcgagc
      781 agaaggcgct gccggaaaaa tccctgctgg cctcggtgcc ggtgggcctg ccgcggccgg
      841 acggcaagtc cggcaacgcg gtggccggct tcgtggtgcc gctgggcacc gacatcaccg
      901 acccggccga gcgcctggcg cagatcaacc gcgtgaccac gcgcagcaag caggagctga
      961 tggaactgcc gcaggccggc ctgatgcagt tcgcgctgct cggcctggcg ccgctgctgc
     1021 tgtcgcaggt taccggcctg ggtgcgcgca tgccgccgat cttcaacttc accatgagca
     1081 acgtggtgct gtccaagcac ccgctgtacc tgatgggcgc ggaactggaa gcgatgtacc
     1141 cggtctcgtt cctgctcgac ggctacggcc tgaacatcac gctggtcggc taccgcgatc
     1201 gcgtggccat cggcgtgctc ggctgccgcg atgccatccc gtcgctgcag cggctggcca
     1261 tctacaccac cgacgcgctg gccgagctgg aggaagccgt cggcgtggtg ccgcgcaagc
     1321 cagtgaagct caagcgccgc aagccggcgc ctgcggcgta accgtcgcgt aaagcccggc
     1381 gcatgcacat ccgcgccttc accctcgccg atgaagcctg cgtcatcgat ctatggcggc
     1441 gctgcgacct gctgcggccg tggaacgacc cgcacaagga catccggcgc aagctgacca
     1501 cgcagcccga gttgttcctg gtcggcgaga tcgacggcca gctcgtcgcc agcgtcatgg
     1561 ccggcttcga cggccaccgc ggctgggtca attacctcgc cgttgcgccg gatcagcgca
     1621 aggccggcct gggccgcacg ctgatgcgcc acgtggaaag cgaactcgaa gcgcgcggct
     1681 gccccaagct gaatctgcag gtgcgcgcca gcaaccaggc ggtgctcgag ttctaccgcc
     1741 gcatcggcta cggcaccgac gacgtggtca gcctg
//
LOCUS       DASZZC010000564         4838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_369187,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000564 DASZZC010000000
VERSION     DASZZC010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369187"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VHE37_11940"
     CDS             <1..935
                     /locus_tag="VHE37_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HVT36293.1"
                     /translation="IPGGGAAAAGTIYQAMAMVNAGLCKHVLIWRAMNERSQYRFGQN
                     PNNTSSYQSGNGTGSLLWCVPYGAMTPASWGALQAARYMHKYGLTNRDLAPMCVQQRK
                     YAATNPKAWFYNKPITIEDHQNSKWIQEPWLRLLDCCQESDGGVAILVTSLERAKDMK
                     QKPVHIVGATQSIPYNVEVISNYYHDDLTVMPEAKGVAKRLWEMTGLKPSDMNAAMLY
                     DAFSPHVLLQMEAFGFVGPGEAAGFIKGGGMALDGKLPTNTHGGLAGEAYIHGMNSMA
                     EGVRQIRGTAVNQVKKAERVLISSGMAGAVLSAV"
     gene            932..1897
                     /locus_tag="VHE37_11945"
     CDS             932..1897
                     /locus_tag="VHE37_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT36294.1"
                     /translation="MSEALSFRLDAGVELPGAGVLHIGASVWLPREARAQIALVCLAG
                     GNMNRRYYDLVAPDGDMSFSYAQAMTARGLVVIALDHLGLGDSSKPADGHALTPQLLT
                     QANVNATGQLLQRLRNGTLADGVAAMPDLLSFGIGHSMGAMMTVLQQHAARQHAGIAL
                     LGFSTRGLPEYLPPAVKELTQEQQRAQLAHYAKKMFPEPYPVIKSRGGGGDIYGSAKV
                     EPKGVQGLKSATDCLIPQGAFMSMLPNNVGPEAAGIDVPVFLGLGERDMAGPPHAIPA
                     AFPKSFDVQLCILPEAGHSHFLFPARALLFERLAAWARMFGGGRA"
     gene            1964..2275
                     /locus_tag="VHE37_11950"
     CDS             1964..2275
                     /locus_tag="VHE37_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36295.1"
                     /translation="MAILKAGSRYKSAVCDTQVMVIKAPAGEFDLRCGGVAMLTGTAT
                     APAGALLDAAHAGETLIGKRYVNADESIELLCTKGGKGSMSIGSEPLQVKQAKQLPSS
                     D"
     gene            2370..3854
                     /locus_tag="VHE37_11955"
     CDS             2370..3854
                     /locus_tag="VHE37_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVT36296.1"
                     /translation="MNISTILDMAAEAFGDRVAVVCGDERYTYAQLRESALRAAASFK
                     ASGAKYAGLLDVNSPAAPVAIFGAAYAGIPYVPLNYRLTAPEINELIERIAPVYLVVG
                     QDYRRLVTPRAGVQLVDRLPFLCLPAPEGEPLQAPDDPRGVAIQLFTSGTTGKPKAAI
                     LRHENLMSYIVGTVEFAGAGEDEANLVTVPPYHIAGISAVLSSTYACRRMVQIEGFSP
                     EGWVAAARAEGATQGFLVPTMLSRIMDHLEQTGDTAVPTLRAISYGGGKMPLSTIQRA
                     MRFWPQVDYTNAYGLTETSATICVLGPQDHRDAAAAGGARLSSVGRPTSAVEMQIRDD
                     AGKILGVGENGLVYVRGGQVSGEYLAQGSLLDGDGWFCTRDRGWLDSEGFLFLDGRAD
                     DVIVRGGENISPGEIEDVLLSHPSVADVAVVAMADEQWGEGVAAAIVLKSGAQASIGE
                     LQELVKSKLRSSRVPQKIVFKDALPYNDMGKVLRRVIRAEFMSG"
     gene            3978..4433
                     /locus_tag="VHE37_11960"
     CDS             3978..4433
                     /locus_tag="VHE37_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVT36297.1"
                     /translation="MFIIEKTIDIDAPAAVVWEVLTDLPRYGEWNPFQVSVKSTLKPG
                     DAIDMQVALMAKPQRQVEWMKEYVEGRRFAYCMKPVPGGALSSFRSHDVEPLPNNRTR
                     YRSYFHLQGWLKPVVLMLFRKNLERGFAGASAGVQRQAESLWAARRGAR"
     gene            4581..>4838
                     /locus_tag="VHE37_11965"
     CDS             4581..>4838
                     /locus_tag="VHE37_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36298.1"
                     /translation="MSEIELTAQRYAQHLLRALGRGDARALAPRMHPALAWARSGAMA
                     LTGSAEREPLMCPAPLAACADGALAAFAALAPAGSCDGIDGA"
BASE COUNT          824 a         1604 c         1661 g          749 t
ORIGIN      
        1 gcatccccgg cggcggcgcg gcggcggcgg gcaccatcta ccaggccatg gccatggtca
       61 acgccggcct gtgcaagcac gtgctgatct ggcgcgcgat gaacgagcgc agccagtacc
      121 gcttcggcca gaacccgaac aacaccagca gctaccagtc cggcaacggc accggttcgc
      181 tgctctggtg cgtaccgtac ggcgcgatga cgccggccag ctggggcgcg ctgcaggccg
      241 cacgctacat gcacaagtac gggcttacca accgcgacct cgcgcccatg tgtgtgcagc
      301 agcgcaagta cgcggcgacc aatcccaagg cctggttcta caacaagccg atcaccatcg
      361 aggaccatca aaacagcaag tggatccagg agccgtggct gcgcctgctc gactgctgcc
      421 aggaatccga cggcggcgtg gcgattctcg tcacctcgct ggaacgcgcg aaggacatga
      481 agcagaagcc tgtgcacatt gtcggcgcca cgcagtcgat tccctataac gtggaagtga
      541 tctccaacta ctaccacgat gacctcaccg tgatgccgga ggccaagggc gtggccaagc
      601 gcctgtggga gatgacgggc ctcaagccgt cggacatgaa cgccgccatg ctctacgacg
      661 cgttctcgcc acacgtgctg ctgcagatgg aggccttcgg cttcgtcggc cccggcgagg
      721 cggcgggttt catcaagggt ggcggcatgg cgctcgacgg caagctgcca accaacacgc
      781 atggcggcct cgccggtgag gcctacatcc acggcatgaa cagcatggcc gagggtgtgc
      841 gccagatccg cggtaccgcg gtgaaccagg tgaagaaggc cgagcgcgtg ctgatttcgt
      901 cgggcatggc gggcgcggtg ctgagcgcag tgtgagcgag gcgctgtcct tcaggctcga
      961 cgcgggtgtc gagctgcccg gcgcaggcgt tctgcacatc ggcgcctcgg tgtggttgcc
     1021 gcgagaggcg cgcgcgcaga ttgcgctcgt gtgcctggcc ggcggcaaca tgaaccgtcg
     1081 ttactacgat ctcgtcgcgc cggacggcga catgtcgttt tcatacgcgc aggccatgac
     1141 cgcgcgcggt ctcgtggtga tcgcgctcga tcatctcggg ctgggcgaca gcagcaagcc
     1201 cgccgacggc cacgcgctga cgccgcaatt gctgacacag gccaacgtga atgccaccgg
     1261 ccagctgctg cagcggctgc gcaacggcac gctggcggat ggtgtcgccg cgatgccgga
     1321 cctgctcagc ttcggcatcg gccattcgat gggcgcgatg atgacggtgc tgcagcagca
     1381 cgcggcgcgc cagcatgcgg gcatcgcgct gctgggcttt tccacgcgcg gcctgcccga
     1441 atacctgccg ccggcggtca aggaactcac gcaggaacag cagcgcgcgc agctggcgca
     1501 ctatgcaaag aaaatgttcc cggagcctta cccggtgatc aagtcgcgcg gcggcggcgg
     1561 cgacatctat ggcagcgcca aggtcgagcc caagggcgtg caggggctga aatcggccac
     1621 cgactgcctg atcccgcagg gcgcgttcat gtccatgctg cccaacaacg tcgggccgga
     1681 agccgccggc atcgacgtgc cggtgttcct cgggctgggc gagcgcgaca tggcgggccc
     1741 gccccacgcc atcccggcgg cattcccgaa atccttcgac gtgcagctgt gcatcctccc
     1801 ggaagccggg cacagccatt tcctgttccc ggcgcgcgcg ctgctgttcg agcgtctggc
     1861 ggcctgggct cgcatgttcg gaggaggacg cgcctgagcg ccgctgccat gctgcgcgcc
     1921 gcgccgggcg acactacgcc cagggactgg tggagaggaa gggatggcaa tactgaaggc
     1981 aggctcgcgc tacaagagcg cggtatgcga cacgcaggtg atggtgatca aggcgccggc
     2041 gggcgagttc gacctgcgct gcggcggtgt ggcgatgctc accggcacgg ccacggcgcc
     2101 ggccggcgcg ctgctcgatg ccgcgcacgc gggcgagacg ctgatcggca agcgctatgt
     2161 gaatgccgac gaatccatcg aactgctgtg caccaagggc ggcaaggggt cgatgtccat
     2221 cggcagcgag ccgctgcagg tcaagcaggc caagcagctg ccgtcttcgg actagggcgc
     2281 gcggcggagc ggcccgcgcc gcgatcctga ttgcgttccc aggcaagaaa aaagcagatc
     2341 gcgcggccgc cggcttttac aatacaacca tgaacatctc gaccatcctg gacatggccg
     2401 ccgaggcctt cggcgaccgc gtcgccgtgg tctgcggcga cgagcgctac acctacgcgc
     2461 agctgcgcga gtccgccctg cgcgcggcgg cgtcgttcaa ggccagcggc gcgaaatacg
     2521 cgggcctgct cgacgtcaac agccccgcgg cgcccgtcgc gattttcggc gcggcctatg
     2581 ccggcattcc gtacgtgccg ctcaattacc ggctcaccgc gccggagatc aacgagctga
     2641 tcgagcgcat cgcgccggtc tacctggtgg tgggccagga ttaccgcagg ctggtgacgc
     2701 cgcgcgccgg cgtgcagctg gtcgaccgcc tgccgttcct ctgcctgccg gcaccggaag
     2761 gcgagccgct gcaggctccg gacgatccgc gcggcgtggc catccagctc tttaccagcg
     2821 gcaccaccgg caagcccaag gccgcgatcc tgcgccacga aaacctgatg agctacatcg
     2881 tcggcacggt ggagttcgcg ggcgccggcg aggacgaagc caacctggtt accgtgccgc
     2941 cttaccacat cgccggcatc tcggcggtgc tgtcgtccac ctacgcgtgc cggcgcatgg
     3001 tgcagatcga aggtttcagc ccggaaggct gggtcgccgc cgcgcgcgcg gaaggcgcaa
     3061 cgcaaggctt cctggtgccg accatgctgt cgcgcatcat ggatcacctc gagcagaccg
     3121 gcgacaccgc ggtgccgacg ctgcgcgcca tctcgtacgg aggcggcaag atgccgctgt
     3181 ccaccatcca gcgcgccatg cggttctggc cgcaggtcga ttacaccaac gcctacggcc
     3241 tgaccgaaac cagcgcgacc atctgcgtgc tcgggccgca ggaccatcgc gacgcggcgg
     3301 cggccggcgg cgcgcgcctg tcctcggtgg gccgtccgac cagcgcggtg gagatgcaga
     3361 tccgcgatga cgccggaaag atcctcggcg tgggcgagaa tggcctggtc tacgtgcgcg
     3421 gcgggcaggt gagcggcgaa tacctggcgc aaggatcgct gctcgacggc gacggctggt
     3481 tctgcacgcg cgaccgcggc tggctcgact ccgagggttt cctgttcctt gacggccgcg
     3541 ccgacgacgt gatcgtgcgc ggcggcgaga acatatcgcc cggcgagatc gaggacgtgc
     3601 tgctgtcgca cccgtccgtc gccgatgtcg ccgtggtggc catggccgac gagcagtggg
     3661 gcgagggcgt ggccgcggcc atcgtgctca agagcggcgc gcaggcgtcg atcggcgaac
     3721 tgcaggagct ggtcaagtcc aagctgcgct cgtcgcgcgt gccgcagaag atcgtgttca
     3781 aggacgcgct gccgtacaac gacatgggca aggtgctgcg gcgggtcatt cgcgcggagt
     3841 tcatgtccgg ctgatcgtca ttgccgcacc cgacagatgc gctcaggtgc aggctgcggc
     3901 gggcctccag ttctcatgcc ggcaaagaaa aaactggatt cccgccttcg cgggaatgac
     3961 ggttggagaa attgaaaatg ttcatcatcg aaaaaaccat cgacatcgac gcgcccgccg
     4021 cggtggtctg ggaagtgctc accgacctgc cgcgctacgg cgaatggaat ccgttccagg
     4081 tctcggtgaa gtccacgctg aagccgggcg atgccatcga catgcaggtg gcgctgatgg
     4141 ccaaaccaca gcggcaggtc gagtggatga aggaatatgt cgagggcagg cgctttgcct
     4201 attgcatgaa gccggtgccg ggcggtgcgc tgtccagttt ccgttcgcac gatgtcgagc
     4261 cgctgcccaa caaccgcacg cgctatcgct cgtactttca cctgcagggc tggctcaagc
     4321 cggtggtgct gatgctgttc cgcaagaatc tcgagcgcgg gtttgccggc gccagcgcgg
     4381 gcgtgcagcg gcaggcggag tcgctgtggg cggcgcggcg cggcgcgcgc tgatgcgcaa
     4441 cgcctgaaaa gcactccacc gcaccgctcg attctctgtt attaattatt gtgtgaaagt
     4501 taaatcacac tactttttat aacactgaaa gcggccgtgc ggtgagggca aaatgcggcc
     4561 ttccagccct gccgcacgcc atgtccgaga tcgaactcac cgcgcaacgc tacgcgcagc
     4621 acctgctgcg cgcgctcggg cgtggcgacg cgcgtgcgct tgcgccgcgg atgcatcccg
     4681 cgctggcatg ggcgcgcagc ggcgcgatgg cgctcaccgg cagcgccgag cgcgagccgc
     4741 tgatgtgccc cgcgccactg gccgcctgcg ccgacggcgc gctggccgcg ttcgccgcgc
     4801 tggcgcccgc tggatcgtgc gatggcatcg acggcgcc
//
LOCUS       DASZZC010000565         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_559210,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000565 DASZZC010000000
VERSION     DASZZC010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559210"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2299
                     /locus_tag="VHE37_11970"
     CDS             <1..2299
                     /locus_tag="VHE37_11970"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36299.1"
                     /translation="VPAAAAGGGGASLAATKSCDDLFKQRVQPRLDFCRNCHVPQGVA
                     DVPDGRLMMLSGDRSQDAANLRASWEQLGTSGTSRILLMASGTDTRSHTGGAPWPAGS
                     RAYLDMAALLAGYGNPSACALSGTSDDKTYPLLGSAHGGHAWFDFCAGKSDDAVLPPD
                     PRSLVRPGVNAGKAVYYNAYWKDCHRDPALVGEDAPARTCGELRTRAAHGAQMVEGNG
                     AVGAGWYFGGDGAAGNLSAADYNTLWQKWGLSARPDNFDELAAQRYGMGLSSKRNPYP
                     LDGEDPNATNGGSGQLPTGLTQLHDADGKWNGNLGVNCHVCHSGQVGTEADGPGLGAL
                     YGNANSLSEFGQIDTDLGKGVAPGVNAPLPLVVGKTRGTNNALALQLIVYLVSQDIRP
                     LDPNFLGFTAFSPNGGSLDAPAWWNVGHRPVKFQDGFLAMDALRSDFGFFLPGPGPGG
                     FDWVRQHVRDGDTWLMSLKSPPYPGAVDTALAEQGAIIFHSKNLWDETLHNPVAKPDG
                     GNGSCASCHGAYSPRYVNDPAYLDTPALEGVASYIVPRDLIATDSARVDSDSEAGEQY
                     GRKDFFAYPETASDDPDLDCTTQNRAEIRQGRLPGYLAPPLYGVWASAPYFHNGSVPT
                     VWEVLQPANRSAIWRRVSTPNRPDQLGLVMGYDTDLARAYDHQRLGWKYDVLACGDAG
                     ATPYLECNPSQDSGPTFLDLLNLIDSHNPFSWYLGLPPSSDQDLENRKIYNTRMYGQG
                     NGGHEFTSVLTDQERRALVEYLKTL"
     gene            2411..3175
                     /locus_tag="VHE37_11975"
     CDS             2411..3175
                     /locus_tag="VHE37_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36300.1"
                     /translation="MNDKLDAATKVPAVTLGFWVIKIAATTLGETGGDTVSMTLGLGY
                     LVGTAIFLGLLALLVALQIAAKKFHPLLYWATIIASTTAGTTMADFADRSLGIGYAGG
                     SLLLLACVIATLALWRATQGSVSVDTVSTPKVEAYYWATITFSQTLGTALGDWIADAG
                     LGYAGGALVFGAALALVAAAYWRTRISHVALFWLAFILSRPLGATVGDFLDKPVADGG
                     MALSRPLASAVLAAFIVACILLIPQRPGTHAAAAKV"
BASE COUNT          555 a         1187 c         1203 g          569 t
ORIGIN      
        1 tgtgcccgcc gcagcggcgg gcggtggcgg ggcgagtctc gcggcaacca aaagctgcga
       61 tgacctgttt aagcagcgcg tgcagccgcg gctggatttc tgccgcaact gccatgtgcc
      121 gcaaggcgtg gccgatgtgc ccgacggcag gctgatgatg ctgtccggcg accgttcgca
      181 ggacgccgcc aacctgcgcg cgtcgtggga gcagctgggc acgagcggca cgtcgcgcat
      241 cctgctgatg gcctcgggca ccgacacgcg ctcgcacacc ggcggtgcgc cgtggcccgc
      301 gggcagccgc gcgtacctgg acatggcggc gctgctggcc ggatacggca atccgtcggc
      361 gtgtgcactg tccggcacca gcgacgacaa gacctatccg ctgctgggca gcgcgcatgg
      421 cgggcacgcg tggttcgatt tctgtgcggg caaatccgac gacgcggtac tgccgcccga
      481 tccgcgctcg ctggtgcggc ccggcgtcaa cgccggcaag gcggtgtact acaacgcgta
      541 ctggaaggac tgccaccgcg atcccgcgct ggtgggcgag gacgcgcccg cgcgcacctg
      601 cggcgagctg cgcacgcgcg cggcgcatgg cgcgcagatg gtcgaaggca acggcgcggt
      661 cggcgcgggc tggtacttcg gcggcgatgg cgctgcgggc aatctttccg cggccgacta
      721 caacacgctc tggcagaagt ggggcctgtc cgcacgtccg gacaatttcg acgagctggc
      781 cgcgcagcgc tacggcatgg ggctttcgag caagcgcaat ccctatccgc tcgacggcga
      841 agatcccaac gccaccaacg gcggctccgg gcagttgccc acgggcctga cgcagctgca
      901 cgacgccgac ggcaagtgga acggcaacct gggcgtgaac tgccatgtct gccacagcgg
      961 gcaggtgggc accgaagcgg atggaccagg cctgggcgcg ctctacggca acgccaactc
     1021 gctgtccgag ttcggccaga tcgacacaga cctgggcaag ggcgtggcgc ccggcgtcaa
     1081 tgcgccgctg ccgctggtgg tgggcaagac gcgcggcacc aacaacgcgc tggcgctgca
     1141 gctgatcgtg tacctggtgt cgcaggacat ccgcccgctc gatcccaact tcctcggctt
     1201 caccgcgttc tcgcccaacg gcggctcgct cgatgcgccg gcgtggtgga acgtgggcca
     1261 ccgcccggtg aaattccagg atggcttcct ggcgatggac gcgctgcgct cggacttcgg
     1321 tttcttcctg cccggccccg ggcccggcgg cttcgactgg gtgcgccagc acgtgcgcga
     1381 cggcgacacc tggctgatgt cgctgaagtc gccgccgtat ccgggcgcgg tggacacggc
     1441 gctggcagag cagggcgcca tcatcttcca cagcaagaac ctgtgggacg agaccctgca
     1501 caacccggtg gccaagcccg acggcggcaa tggctcgtgc gcgtcgtgcc acggcgcgta
     1561 ctcgccgcgc tacgtcaacg atcccgcata tctcgacacg ccggcgctgg aaggcgtggc
     1621 cagctacatc gtgccgcgcg atctcatcgc caccgacagc gcgcgcgtgg acagcgacag
     1681 cgaagccggc gagcagtacg gccgcaagga tttcttcgcg tatcccgaga ctgccagcga
     1741 cgatcccgat ctcgactgca ccacgcagaa ccgcgccgag atccgccagg gccgcctgcc
     1801 gggctatctc gcgccgccgc tttacggcgt gtgggccagc gcgccgtatt tccacaacgg
     1861 ctcggtgccc acggtgtggg aagtgctgca gccggcgaat cgcagcgcca tctggcggcg
     1921 cgtgtcgacg cccaaccgtc ccgaccagct cggcctcgtg atgggttacg acaccgatct
     1981 tgcacgcgcg tacgatcatc agcgcctggg ctggaagtac gacgtgctcg cctgcggaga
     2041 cgccggcgcc acgccgtacc tcgagtgcaa tccgtcgcag gacagcgggc cgacctttct
     2101 cgacctgctc aacctgatcg acagccacaa tccgttctcc tggtacctgg gcctgccgcc
     2161 cagcagcgac caggacctgg agaaccgcaa gatctacaac acgcgcatgt acgggcaggg
     2221 caacggcggc cacgaattta cttccgtgct gacggatcag gagcgccgcg cgctggtcga
     2281 atacctgaaa acgctctgac cgcaccgcct ctggtaatgt tattgattat tgattgattg
     2341 taaaataaat cgatatttaa taacgcgccg tagagggatg cggtatccct cctgctacgg
     2401 atttcgaagc atgaacgaca agctcgatgc agccaccaag gtgccggccg tcacgctggg
     2461 cttctgggtc atcaagatcg ccgctaccac gctgggcgag accggcggcg acacggtgag
     2521 catgacgctc ggcctcgggt atctcgtcgg cacggcgatc ttcctcggcc tgctggcgct
     2581 gctggtggcg ctgcagatcg cggcgaagaa attccacccg ctgctctact gggccaccat
     2641 catcgcctcg accaccgccg gcacgaccat ggcggatttc gccgaccgct cgctgggcat
     2701 cggctacgcc ggcggctcgc tgctgctgct ggcctgcgtc atcgccacgc tggcgctgtg
     2761 gcgcgccacg cagggcagcg tgtcggtgga caccgtgtcg acgccgaagg ttgaggcgta
     2821 ctactgggcc accatcacgt tttcgcagac gctcggcact gcgctgggcg actggattgc
     2881 cgacgccgga ctgggctacg ccggcggcgc gctggtgttc ggcgcggcgc tggcgctggt
     2941 cgcggcagcg tactggcgca cgcgcatctc gcacgtcgcg ctgttctggc tggccttcat
     3001 cctgtcgcgc ccgctcggcg ccaccgtcgg cgatttcctc gacaagccgg tggccgatgg
     3061 cggcatggcg ctgagccggc cgctggcatc ggcggtgctg gcggcgttca tcgtcgcctg
     3121 catcctgctc attccgcagc ggccgggtac gcatgcggca gcggcgaagg tttgaggttt
     3181 ttagtgcgcg gcgagcgctt cggggctcga ggtcgttccc aactctccag cgaatcgcac
     3241 aggattgtct cctctcccct tgcgggagag ggctagggtg agggaagagc gccgggagag
     3301 cgagcgtaac gagtgctttg tgcgcgccgc gcatgctgtt gacggtgggc cccattcgac
     3361 gcgccgagaa gcgcagacgc aggcggggct gtccgtggct gactgtccga cgctctgcat
     3421 tccggcagga atgcagggct agtttcagcc acggccgcct gtgtcgagca tcgcagggca
     3481 gtcgcgccag cgaccgcggc ggctggggtg cgct
//
LOCUS       DASZZC010000566         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_107398,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000566 DASZZC010000000
VERSION     DASZZC010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107398"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..2054
                     /gene="pepF"
                     /locus_tag="VHE37_11980"
     CDS             180..2054
                     /gene="pepF"
                     /locus_tag="VHE37_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HVT36301.1"
                     /translation="MRRMLLLAACCVAASGAARAETEADRWSLADLYPNVAAWNADAD
                     KLSAQYKVFAACQGQLHTGAARFRQCLELQADIDKRYARLETYASQLLDENTDVASSL
                     ELSQRADLLGTQRDEAESFMAPEILTLGAKKVNAYLKQDPKLAIYRHPLDVILRAAPH
                     TLDQKGEALLASFGMVTDTPSQVYGILSSADVPWPTIKLADGSDAHLDQAGYTRYRAV
                     QNRDDRKRVYDAFWGKWKEYERTFGVTFYARLKTSSVYARARNYPDSISAALDANHVP
                     VAVYDTLLKETNNNLPTLHRYFKLRAKLLGVDQLNYYDIYPPIVKSDLKFPIDEGRRL
                     MLEAVKPLGDDYVAAVAKGLKDRWMDVYPRPHKRSGAHEEGAAYDVHPLLLINYNDDY
                     ESVSTITHEWGHAMHTYLANHAQPFVTASYPIFVAEIASTLNEALLLDYVVKNAKNDD
                     ERLYYLGSALENLRATYFRQAMFADFERQVHAQVDQGQSLTGEAITKIYGDILKRYSG
                     DAQGVVKIDDAYTVEWAYIPHFYNPYYVFQYATSIAASSQFVDEILSGKPGARERYLN
                     LLKAGGSDDPYTLVKAAGVDLATPAPYAALAARMDRIMDQIETLLARREKPAAAPPAP
                     "
     gene            complement(2065..2349)
                     /locus_tag="VHE37_11985"
     CDS             complement(2065..2349)
                     /locus_tag="VHE37_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36302.1"
                     /translation="MYTAPIKTGGYFTLTQVCLRPNDGVALQGNTVGIIVRRQSGDAT
                     ESENVLGSCVTFTPGLVIPPSEQLSCVNTLGSGPSWCMLTGIKSPPDSTP"
BASE COUNT          482 a          852 c          799 g          390 t
ORIGIN      
        1 cagaacccgc agctgcagct gcgcagcgga agcgcgtcgg ctgcaagctg cgtctcgaac
       61 agcaaggcga gattgccgca gtggcagcga ccctggtagg tgtgcgtcat gcccgcagta
      121 tgccgcgcgc agcggacccg ggtgcataat cgccgccagc caaaatctca ggagtgccca
      181 tgcgccgcat gctcttgctc gccgcctgct gtgttgccgc cagcggcgct gcccgcgcgg
      241 aaaccgaagc cgaccgctgg agcctggccg acctctaccc gaacgtggcg gcgtggaacg
      301 ccgacgccga caagctgtcg gcgcagtaca aggtgttcgc cgcctgccag ggccagctgc
      361 acaccggcgc ggcgcgtttt cgccaatgcc tggagctgca ggccgacatc gacaagcgct
      421 acgcgcggct ggagacgtac gcctcgcagc tgctcgatga aaacaccgac gtcgcatcca
      481 gcctggaact gtcgcagcgc gccgacctgc tcggcacgca gcgcgacgag gcggagagct
      541 tcatggcgcc ggaaatcctc acgctcggcg cgaagaaggt gaacgcgtac ctgaagcagg
      601 acccgaaact ggccatctac cgccatccgc tcgacgtcat cctgcgcgct gcgccgcata
      661 cgctggacca gaagggcgag gcactgctcg ccagcttcgg catggtcacc gacacgccgt
      721 cgcaggtgta cggcattctt tcctcggccg acgtgccgtg gccgaccatc aagctggccg
      781 acggcagcga tgcgcatctg gaccaggccg gctacacccg gtaccgggcg gtgcagaacc
      841 gcgatgaccg caagcgcgta tacgacgcct tctggggcaa gtggaaggaa tacgagcgca
      901 ccttcggcgt caccttctac gcgcggctga agaccagttc ggtgtacgcc agggcgcgca
      961 actacccgga ctcgatcagc gccgcgctgg acgccaacca cgtgccggtg gcggtgtacg
     1021 acaccctgct gaaggaaacc aacaacaacc tgccgacgct gcaccgctac ttcaagctgc
     1081 gcgcgaagct gctgggcgtc gaccagctca attactacga catttacccg ccgatcgtga
     1141 agagcgatct gaaattcccc atcgacgaag gccggcgcct gatgcttgag gcggtgaagc
     1201 cgctgggcga cgactacgtg gccgcggtgg ccaaggggct gaaggaccgc tggatggacg
     1261 tctacccgcg tccgcacaag cgcagcggcg cccacgagga aggcgcggct tatgacgtgc
     1321 acccgctgct gctgatcaac tacaacgacg actacgagtc ggtgtcgacc atcacccacg
     1381 agtggggtca cgccatgcac acctacctgg ccaaccacgc gcagccattc gtcaccgcca
     1441 gctacccgat cttcgtcgcc gagatcgcct ccacgctcaa cgaggcgctg ctgctcgact
     1501 acgtggtgaa gaacgcgaag aacgacgacg agcgcctgta ctacctgggc tcggcgctgg
     1561 agaacctgcg cgccacctac ttccgccagg ccatgttcgc cgacttcgaa cggcaggtgc
     1621 atgcgcaggt ggaccagggc cagtcgctca ccggcgaggc catcacgaaa atctacggcg
     1681 acatcctcaa gcgctacagc ggcgatgcgc agggcgtggt gaagatcgac gacgcctaca
     1741 cggtggagtg ggcctacatc ccgcacttct acaacccgta ctacgtgttc cagtacgcca
     1801 cctcgatcgc cgccagcagc cagttcgtcg acgagatcct gtccggcaag ccgggcgcgc
     1861 gcgagcgcta tctcaacctg ctcaaggccg gcggttcaga tgatccgtac accctggtga
     1921 aagcggcagg cgtggatctc gcaacgccgg cgccatacgc ggcgctggcc gcacgcatgg
     1981 accgcatcat ggatcagatc gaaacgctgc tcgcccggcg cgagaaacct gcggctgcgc
     2041 cacccgcgcc ctgaccgcgg cgggtcaggg cgtgctgtcg ggcggactct tgatgccggt
     2101 gagcatgcac cacgacggcc ctgagcccag cgtgttgacg catgacagct gctcgctggg
     2161 gggaatcacc agccccggcg tgaaggtgac gcaggagccg agcacatttt cggattccgt
     2221 ggcatcgccg ctctggcgac gcacgatgat gccaacggta ttgccctgca gcgccacgcc
     2281 gtcgttgggc cgcaggcaaa cctgggtcag cgtgaaatag ccgccggtct tgatcggcgc
     2341 cgtgtacatc aggctattgg ttgaatcacc gtatgcactg agcagttggc cggcaatggg
     2401 tccgctgacc ggggcgccga gcttgccggc tgcgccggcc gtgcagacgg tgatgaaggc
     2461 caatgctccg gcaatccatg ccgaacgctg gaagtttttt tttcatggtg cagtcctctc
     2521 cgt
//
LOCUS       DASZZC010000567        17002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_301422,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000567 DASZZC010000000
VERSION     DASZZC010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301422"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VHE37_11990"
     CDS             complement(<1..1274)
                     /locus_tag="VHE37_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HVT36303.1"
                     /translation="MKAPATTQRADFDKLNAVDRVIAYFSPSFGIRRQQQRAALAAMR
                     GYESAEPSRKRRFHYNKSDGNVLARMSAVALRDQARHLERNHDITVSILDKLVDFTVG
                     TGIVTQFQPKRRDGSIHTEFAALLTRKHAKWSEWPEVTWTHARGAAERLACRTMYRDG
                     EVLGQMIFGPRSDNPYSMEVPFAIEMMEADLLPYELNNLDANVRQGIQCNAWGRPQIY
                     NVYKNHPGGDFVALTLQTKPIPAENILHPRLVNRIGQRRGITILAPIIARAQDLHEYE
                     GQEFAAAKMAASLVLKMTRGSPDMWDNAAQTYDKDNPPVMQMDGGMVVVQNQQGENTE
                     FFDTKRPNAAVEPFSEMNVRRIAGGVGLSYSAVSRNYNGTYSAQRQELVENWPHYHAM
                     TGIWVAQWNRPADQRFIDWVVLTQGMPADVDPA"
     gene            complement(1271..1498)
                     /locus_tag="VHE37_11995"
     CDS             complement(1271..1498)
                     /locus_tag="VHE37_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36304.1"
                     /translation="MSDTADRLQRYKDAETRILKGQQFRMDGRILTLADLAEVTKQID
                     KLSAQLAAETSAAKGNYGPRVMVADFSKPLR"
     gene            1590..2255
                     /locus_tag="VHE37_12000"
     CDS             1590..2255
                     /locus_tag="VHE37_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36305.1"
                     /translation="MSEDVIGPSRSFAAFEEAIIDLRKRSLRARVSFLMVAGGMAMSI
                     VFIVTWIVANTSLLRARTSEINFNNDPSSLEHELNNAGSDLVRRLLADDLSPEKRKEL
                     ERELERNVELLKALPTQQPIIERSKGIADYVPAFAFSIGGVGFVVLLIQIAVQFLRYF
                     ARLSELYAAQATAMIASAGDVQNAILFMQNFSPASIDIGKAPSTIYEKALDTIAAVAK
                     KND"
     gene            complement(2282..2869)
                     /locus_tag="VHE37_12005"
     CDS             complement(2282..2869)
                     /locus_tag="VHE37_12005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT36306.1"
                     /translation="MKLPTVDPATLNVQPIDWTGLDQRYMNPGELEILIALIRSVNPR
                     GVLEIGVNSGRTAKAILDNVPGIEHYQGVDVLPGYKTERRVQRREVPETPGALAARDG
                     RFELVLRSRGSLDLCAADLRPCDAVFIDGDHGRKAVTHDTKLARQLVRPGGIIIWHDY
                     HDLDVVDVRAVLDELYDQGWPLNHLTNTWIAFSAG"
     gene            complement(2850..3764)
                     /locus_tag="VHE37_12010"
     CDS             complement(2850..3764)
                     /locus_tag="VHE37_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36307.1"
                     /translation="MTHLHIKGMHGLGDNLHQRGIIRQLMQRHTVWLETPWPCVYHDL
                     VREGLLLVATESQLRTQSKNAERERAMFHADRAPATAEQLRIFYTPQEVRAQGSVLGA
                     MASHCGVSVDAADFRLPIPQAWHDQAAPWLARWNPSRPIAIYRPLNERTEWGGCAARN
                     PDHGAYAQLIQSVRHDYFIVSVADFVRGEEWMVGHDIKADTECHAGELPFETLAALAS
                     NALFVFTSPGFAAILAQAVGTPLVCVFGGYESSRSFSAGAKYADYLGIDTISPCECFE
                     HKHACEKHIDLDAVIPRLRNFLHEAAHR"
     gene            complement(3761..4381)
                     /locus_tag="VHE37_12015"
     CDS             complement(3761..4381)
                     /locus_tag="VHE37_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36308.1"
                     /translation="MTFARESSPLSFGDSARSAWIVATGPSATALPKDLHPPSTIAVN
                     DAWQLVPDAAACYAGDIEWWRQHGALCARGFTGERWTSANPAADYLPVRQVSWRRGNT
                     MPPEPGCICFGAGAGKSSTLQAMSLAVLWGAGRLVLVGVDLGVVGGMAHFFGEHPPAL
                     RKRKSHFEHQRRAIGQLACDLAGRGIQVINLSPHSRICGTTHPEQP"
     gene            complement(4378..4683)
                     /locus_tag="VHE37_12020"
     CDS             complement(4378..4683)
                     /locus_tag="VHE37_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36309.1"
                     /translation="MTRYRQDHLAVLRSRITHLVRQATGESEQIATRVADQVIESVQH
                     EFGGQVLYFRQRARYSIAAIISEIEQGAKRKDICRRHRISTATYYRILHQRRQAVAS"
     gene            complement(4680..6689)
                     /locus_tag="VHE37_12025"
     CDS             complement(4680..6689)
                     /locus_tag="VHE37_12025"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HVT36310.1"
                     /translation="MSIMAQLSGRTIDSLRRARRRFFPKERLSTAQWTDRYRRLAGGL
                     LPGKFLFHNQPALEGILDAFDDPYVREVWGQKCAQFGWTQGVTLNVIFKRVHLQPSPI
                     LVLFPKDGSGKRFMREKVEPEARATPEIAARINTGARRPDNTQDYKAFPGGYLQLVGT
                     NSPANVKSTDAPLVFVEEPDDTSKDLRGQGDSVALGRERVKSYGEHGKLGVGGTPTVE
                     GLSKVAAGMERSDKRRYYVACPHCGHEQTLRWDQVDWQSDALLSHPVYGTHRPETARY
                     RCEADECGALWSDAEKNAAVRAAAGRADKGWRATAPFNGIAGFYINELMSVSVGSPME
                     LLVRKFLEASHKLKSGDDSHMRSFVNNQLGETWQLKSDAPEVEQLRERAESYAQWTTP
                     AGGLICVASVDVQRGGESGEPRLEYVVKAFGRGMESWLVAYGVVPGNPLEPATWDALD
                     PILNKPVRNLGGGTLGISAVSIDSSDGMTQDAVYRYARRRKHRGFMAIKGSANPHKEI
                     FSPPSQSIDTNATDKAAKYGLKLYWVGVEKAKDLISGRIKLAGDGPGRMHFCADLGDN
                     YFAGLTSEVKVPGPRGRPVWTKKAGARNEPLDLEVYALHAAHKLRVHTWTESQWVAVE
                     EKVRQRELLEDPRQGDAPATVATVPRGTSPVAGTRFEGFGSRRGL"
     gene            complement(6686..7438)
                     /locus_tag="VHE37_12030"
     CDS             complement(6686..7438)
                     /locus_tag="VHE37_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36311.1"
                     /translation="MIDQALTGRQLVAELAKIGVGTWSADAVRQWIREEPPCPIAEHA
                     DQGKPHRYSLAQVLAWLRIRAMAERAKGFTRGDGADLVSRIDRALSGAQPSLLAGVEP
                     EQEAPEFSAARIAPTPGAGAAPTPKAPEPEWLQLSDRDALLEVLKGRDPRNWKAAEEA
                     ITIRTKRMEAQRELIPAPELDAALELQVTHIRQAIVEAGSALKSIMRDLVPPERTQQM
                     VRVIDDAVTQLLSRLASDSDLPIAPGSESAAA"
     gene            complement(7910..10447)
                     /locus_tag="VHE37_12035"
     CDS             complement(7910..10447)
                     /locus_tag="VHE37_12035"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HVT36312.1"
                     /translation="MTEPLAKSPERNLADARVFLGALDAAGASFTFQTFDDSPSKRKS
                     LGQIVHGTLDERWRWLSRMNDQGAGVFVTVNATDGEGREARNITRVRALFVDLDGAPL
                     DPVMQAPLLPQIVVESSPGRWHAYWLVRDCPRKDFRQYQRGLIARFQADKSVHDLPRV
                     MRVPGFIHRKHEPFVSRLHALHELPPYDIAALVLAFELEKPLAPELPAASAAKAAPAP
                     LPQSTADDVADDLRDALRSIGSDDYGTWISIGMALATLGNAGMPLWLEWSSKSAKYDP
                     QAALDKWKSFKPEQTDHKVVFAEAKKHGWDPRRAPSVQRKRAARAPARPERVPAPGDP
                     ADAAAPAPAPPQNSPEALLQRYELIYGTKNVWDCELGSEMSFSAFTALVGKAAASGWL
                     ENDKRRVRMLPGAPKKTGKKDKREPPPFSTKVEWMLPRYALIYSEEAVFDREKRIEMT
                     LGALRAFAGLKAVRDWGDHPKREVVNKEQVIFDPKRDVDDPSICNLWGGWPRIPKKGT
                     PADEALVDRWMKVVHYCCGESDAITDWVLRWIAYPLRYPGTKMRTSIIMHGPEGSGKN
                     TIWDAVRRIFGRYGIQITQTQLEQQWCDWISAKLFIVGNEVLHRQEQVQQKGRLKTMI
                     SDDTLRVERKFMNGRDEINFANLVFLSNELQPLSIDPGDRRFLVIWTPPPHPDGYDYY
                     RGLGADAMPESAVDALYHRLLQLDLEDFGPHTKPPMTAAKQDLIDASMESNQRFLRDW
                     IAGALPVPYGPCKTQQLFHAYLYWCRENGERFPKPENKFAARAKKDVPAAVKWYLVGQ
                     SKKQGTFYLPRGINPPPQNEKSEAAWLSDGMERFRREFEEWKNEHGS"
     gene            complement(10444..10662)
                     /locus_tag="VHE37_12040"
     CDS             complement(10444..10662)
                     /locus_tag="VHE37_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HVT36313.1"
                     /translation="MDAVQERQLAELEDLNARARVTTLPPQKLRRRCCRRCGEPIPPA
                     RLKAVPLADHCIDCGELNELRDSQRRRA"
     gene            complement(10710..11288)
                     /locus_tag="VHE37_12045"
     CDS             complement(10710..11288)
                     /locus_tag="VHE37_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36314.1"
                     /translation="MKNSAQRVGLDETRSETVSRFFDLVMRKTRMSKETFAQLVLEQY
                     QARVPEPARSFDFSADADVFRRAKVNGQHIGRLMDGTTRMPSDLEESVVFAFPEPYRS
                     ECVRDLALRYGLLDVPVPANTQISDMQRLSRLSSEFARVLDALGPMLDDGRLDQADAP
                     HAELAMRELRGLLAAGEGLIARIEQSVRPGSE"
     gene            complement(11508..11747)
                     /locus_tag="VHE37_12050"
     CDS             complement(11508..11747)
                     /locus_tag="VHE37_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HVT36315.1"
                     /translation="MAIAEYLREKNLTQAQFAELVGVTQSMVAQWVGGIRRISAERAI
                     AIEEKTGRDLRKGQLRPDLWPDETAAASDSDRKAA"
     gene            11828..12544
                     /locus_tag="VHE37_12055"
     CDS             11828..12544
                     /locus_tag="VHE37_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HVT36316.1"
                     /translation="MLKRKPLTQDELDAAENLRQLFTEAKAADKTLTQERLAFQFGWK
                     TQSAVSQYLRGVIPLNSDAALKFADFFRVAPSQIHPMFSRLDRAAIAEPKTKYGTELH
                     LREIPVVGRGKGGEDGFFEEEDFPVGHGNEVILHASADHNAFCVRVEGDSMSPRYRHG
                     EYVLVEPNQPGQAGDDVYVVLRNGRKLIKELAYMRDSEIKLISVNATDHPPRTFPLSE
                     VDSIYRIGGSIRRGSGFVRS"
     gene            12574..12837
                     /locus_tag="VHE37_12060"
     CDS             12574..12837
                     /locus_tag="VHE37_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36317.1"
                     /translation="MAPKLMICTSCYEFTFGTTKTRGSFLVEVVLWLCFLLPGVIYSL
                     WRLSTRHDACDKCGGPIIPADTTRGRALAKQYHSDGMATPRAA"
     gene            complement(12838..13626)
                     /locus_tag="VHE37_12065"
     CDS             complement(12838..13626)
                     /locus_tag="VHE37_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVT36318.1"
                     /translation="MSWVPKQRVPKLLTGLTTKVSSNAADEGIVDAGGSGVPFTQTMY
                     LYYRDAKGDESKRRVTTRCLRPWGGGDDLAMIAWCHERDALRTFLASRMQELVDLNTG
                     ESHRPPAPWLWQQYRSWPDHEQFEAEPAAATERPAEPQVLPAIASPANRLRLARRAVL
                     CMVYVARSDGRMDMTERGVIAKFLRNAERNLGVEFPWSDVHGQLQRLSCDQGEFLVGI
                     KQLRSGPAEFRTLLRNAVTELVAADGNADPEEQRASRWLEVMMA"
     gene            13768..14049
                     /locus_tag="VHE37_12070"
     CDS             13768..14049
                     /locus_tag="VHE37_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36319.1"
                     /translation="MQQTLFHTCGARVDVNEVIEGRSNRFVFLDALTGDGLDRCPRCS
                     RELHIHAFRPQAPLPKLSRAKAARPAGAKREARRLIAKARRIRTQRSAA"
     gene            14046..14555
                     /locus_tag="VHE37_12075"
     CDS             14046..14555
                     /locus_tag="VHE37_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36320.1"
                     /translation="MSAAPLHTPEPWPDSASADYARQRLCQLQEFAHQQVGMQHSCDC
                     CCGHGGFWRDKNSRYGGTFAEGYRNDGAAIQWLEIAARACAGMPDPAAEIERLLGEHH
                     TASGAYALLEHEAEALRASNAELLAALKDARETIRTWHGPVAWEIYDKESPEMKRLNS
                     AIAKAEGRA"
     gene            14552..14773
                     /locus_tag="VHE37_12080"
     CDS             14552..14773
                     /locus_tag="VHE37_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36321.1"
                     /translation="MNVPLNQLYVIIDLKHGVTESELAEAVKLLAAKTHGDINAAYRV
                     VTPPRRTWWERFTQLVTGAPVYALRQVPL"
     gene            14770..14991
                     /locus_tag="VHE37_12085"
     CDS             14770..14991
                     /locus_tag="VHE37_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36322.1"
                     /translation="MTPQPRLALLASHQGEACALILQSLQTTHADVLRFDDISRAETR
                     DGVATVVGDFTHLPSMRRYVIAIAAVPLD"
     gene            15004..15315
                     /locus_tag="VHE37_12090"
     CDS             15004..15315
                     /locus_tag="VHE37_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36323.1"
                     /translation="MNHLKVVTTQRQPELDFQAQAQELVARRNEHLCHALSLYERAEE
                     EYKFAQKALDHARQRRDEARAQLTHLVGRDEKLLVGNVLLTRHANGGLHIESVSVVGL
                     V"
     gene            15326..16825
                     /locus_tag="VHE37_12095"
     CDS             15326..16825
                     /locus_tag="VHE37_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVT36324.1"
                     /translation="MPEASTFARETAAMPVEQISLDLISVQDQIRKNFDPDKLEQLGQ
                     SIKQHGLVQPIVVAPENNGTGMRLLMGERRLRAAKAAGLTTLPTIVRHDLAGQDVRVL
                     QAIENLQREDLTMAEECAGVTALVESIGFEATCQQLCKSESWVSKRSTIKQLPAPIKD
                     LVEQGKVKDLEVVHLLGQLAKHNSTKAFELARMARVVDDAGNLPAHQQPTRENISALV
                     RAERETAERKQQEKIERDAAKNDPAAIKAKKRDASAKEERKQLLAKLQQLKDAGTALE
                     DETMEALGKALGIKVPPRGEFGGRGHCDVMVAMDYLQEHMKLPASVDGAGFTFRADGS
                     VSLLKSVCKLTAHAQKLAITIREVSVDQAEKIAAIVGEKAVTVGLVKRVNGKELRSYI
                     ERARAAKHEAKTEKREAAKEGPEYQLAKLREFLRKHVTVIGGKEARLNTVTAKAMYGA
                     YAAYCQANKVEPLSLNDNAYGAAILAHAGGGIVKKRTNTGYVYVGCSLA"
     gene            16822..>17002
                     /locus_tag="VHE37_12100"
     CDS             16822..>17002
                     /locus_tag="VHE37_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36325.1"
                     /translation="MSAVTAESNLQLVAQASELMRLAFEIANDSAQSDVECMCHIHSG
                     NADEGWWYELGKPSPE"
BASE COUNT         3091 a         5405 c         5373 g         3133 t
ORIGIN      
        1 gccgggtcga catccgccgg catgccctgg gtgagcacca cccagtcgat gaatcgctgg
       61 tcggccggac ggttccactg cgcgacccag atgccggtca tggcatggta gtgcggccag
      121 ttctccacca gctcctgccg ctgcgcggaa taggtgccgt tgtagttgcg cgagaccgcc
      181 gagtagctca ggccgacgcc gccggcgatg cggcgcacgt tcatctcgga gaacggttcg
      241 acggcggcgt tgggacgctt ggtgtcgaag aactcggtat tttcgccctg ctggttctgc
      301 accacgacca tgccgccgtc catctgcatg accggcgggt tgtccttgtc gtaggtttgc
      361 gccgcgttgt cccacatgtc cggcgagccg cgcgtcatct tgagcacgag gctggcggcc
      421 atcttggcgg cggcgaactc ctgcccttcg tactcgtgca ggtcctgcgc gcgcgcaatg
      481 atgggcgcga gaatggtgat gccgcggcgc tgcccgatgc ggttgaccag gcgggggtgc
      541 aggatgttct cggcggggat tggcttggtc tgcaaggtga gcgcgacaaa gtcaccgccg
      601 gggtgattct tgtagacgtt gtaaatctgc ggccggcccc aggcgttgca ctggatgccc
      661 tggcggacgt tggcgtcgag gttgttcagc tcgtacggca gcaggtccgc ttccatcatc
      721 tcgatggcaa acggcacttc catgctgtac gggttgtcgc tgcgcgggcc gaagatcatc
      781 tggcccagca cctcaccgtc gcggtacatg gtgcggcagg ccagccgctc ggctgcgccg
      841 cgcgcgtgcg tccaggtgac ttccggccac tccgaccact tggcgtgctt gcgcgtgagc
      901 agggcggcga attcggtatg gatggagcca tcgcgccgct tcggctggaa ctgcgtgacg
      961 atgccggtgc cgacggtgaa gtcgaccagc ttgtccagga tgctgacggt gatgtcgtgg
     1021 ttgcgttcca ggtgacgcgc ctggtcacgc agggcgacgg cggacatgcg cgcgagcacg
     1081 ttcccgtctg acttgttgta gtggaagcgg cgcttgcgcg aaggctccgc cgattcgtaa
     1141 ccgcgcattg ccgccagcgc ggcccgctgt tgctggcgcc tgatgccgaa ggagggcgag
     1201 aaataggcga tgacgcgatc gacggcgttg agcttgtcga agtctgcgcg ctgcgtggtg
     1261 gccggagctt tcatcgaagc ggcttcgaga aatccgcaac catgacgcgc ggtccgtagt
     1321 tgcccttggc tgcgctggtc tccgcggcga gttgcgcgga gagcttgtcg atctgcttgg
     1381 tgacctccgc caggtcagca agcgtgagga tgcgaccgtc catgcggaac tgctggccct
     1441 tgaggatgcg ggtttcggca tccttgtagc gttgcaggcg gtcggcggtg tcactcatgc
     1501 ccggcagggt gccggccggg gtttctcatt tacatataaa gcgtgagaat attttcatcc
     1561 acaggattct cgcgcttggg gggggggaca tgagcgaaga cgtaattggg ccttcacgat
     1621 cgttcgctgc gtttgaggaa gcgataattg atttgcgaaa acgatctctg cgagcgcggg
     1681 tcagcttttt gatggtggcc ggggggatgg ccatgagcat cgtcttcatc gtcacttgga
     1741 tcgtggccaa tacaagcctt ctccgagcgc ggacgagtga aattaatttc aacaacgacc
     1801 catctagcct ggagcacgag ctcaacaacg ccggctctga ccttgtccgc aggttactgg
     1861 ccgatgacct ttctccagaa aaaagaaagg aactggaacg cgagcttgaa agaaatgttg
     1921 aattgctgaa ggcgctccca acacaacaac cgatcatcga acgctctaaa ggaattgcgg
     1981 actacgttcc agctttcgcg ttcagcatcg gcggcgtggg cttcgtcgtc cttttgatac
     2041 aaatagccgt gcagtttttg cgatatttcg ctcgattgtc ggaactatat gccgcccagg
     2101 cgacggcgat gatcgcctcg gccggtgacg tgcagaatgc gattctcttt atgcagaact
     2161 tttcgccggc atcgattgat atcgggaagg cgccatcaac catctacgag aaagcgcttg
     2221 atacgattgc cgcggtcgca aagaaaaacg attgagcgag tggggttatg aatataacgt
     2281 gctatcccgc actaaaagcg atccaggtgt ttgtcaggtg gttcagcggc catccttgat
     2341 cataaagctc gtcaagtacc gcgcgtacat caacgacgtc taggtcgtga tagtcatgcc
     2401 agatgatgat gccgccgggc cggacgagtt gccgcgcgag cttcgtgtca tgtgtcactg
     2461 ccttccgacc atgatcgcca tcgatgaaga ctgcatcgca cggccgcaaa tcggcagcgc
     2521 ataggtccag cgatccgcgc gagcgaagga ccaactcgaa gcggccgtcg cgcgcggcaa
     2581 gcgcgccggg cgtttccggg acttccctgc gctgcacgcg gcgctcggtc ttatagccgg
     2641 gcagtacgtc gacgccctgg tagtgttcga tgccgggcac gttgtccagg atggccttgg
     2701 cggtgcgccc gctgttgacg ccgatctcca gcacgccgcg cgggttcacg ctgcgaatca
     2761 gcgcgatgag gatctccagc tccccagggt tcatgtagcg ctgatcgagc ccggtccagt
     2821 cgatgggctg gacattcagg gtggcggggt caacggtggg cagcttcatg gagaaaattc
     2881 ctcagacggg ggatgacagc gtcaagatcg atgtgctttt cgcaggcgtg cttgtgctca
     2941 aagcactcgc agggagagat agtgtcgatg cccaggtagt cggcatattt cgcacccgcc
     3001 gaaaacgagc ggctgctttc gtagccgccg aacacgcaga ccaggggcgt gcctacagcc
     3061 tgagcgagga tggcggcgaa cccgggcgaa gtgaaaacga acaaggcgtt ggacgcgagc
     3121 gcagccagcg tctcgaatgg cagttcaccg gcatggcatt cggtgtcggc tttgatgtca
     3181 tggcccacca tccattcttc gccccgtacg aagtctgcga cgctaacgat gaagtagtcg
     3241 tgccgcacgc tctggatgag ctgggcatag gcgccatggt ccgggttgcg ggccgcgcag
     3301 ccgccccatt ccgtgcgttc gttaagcggg cgatagatgg cgatgggacg cgacgggttc
     3361 cagcgcgcca gccatggggc tgcctggtcg tgccatgcct gcgggatggg caggcgaaaa
     3421 tcggcagcat cgacgctgac gccgcaatgg ctggccatgg ctccgagcac ggagccttgt
     3481 gcgcgcacct cctgcggcgt gtagaagatg cgcagctgct cggcggtggc tggcgcgcgg
     3541 tccgcgtgga acattgcgcg ctcgcgttcc gcgtttttgg actgggtgcg taactgcgat
     3601 tcggtggcta ccagcagcaa gccttctcgc accaggtcgt gatagacgca cggccatggc
     3661 gtttcaagcc agacggtatg gcgctgcatg agctggcgga tgatgccgcg ctggtggagg
     3721 ttgtcgccca ggccgtgcat ccccttgatg tgcagatgcg tcatggttgc tccgggtggg
     3781 tagtgccgca gatccggcta tgcggcgaaa gattgatgac ctgaatgccg cggcctgcca
     3841 ggtcacaggc gagctggcca atggctctgc gctgatgctc aaagtgagac ttgcgcttgc
     3901 gaagtgccgg cgggtgttcg ccgaagaaat gcgccatgcc gcccacgacc cctagatcca
     3961 caccgacgag caccaggcgg cctgcacccc aaagcaccgc cagggacatg gcctgcagcg
     4021 tgctgctttt gccggcgccg gctccaaagc agatgcagcc aggctccggc ggcatggtat
     4081 tgccgcggcg ccacgacacc tggcgcacag gcaggtagtc tgccgctggg ttggcactgg
     4141 tccagcgctc gccggtaaat ccgcgcgcgc agagagcacc gtgctggcgc caccactcga
     4201 tgtcgccggc atagcaggcg gcggcatccg gaacgagctg ccaggcatca ttcaccgcga
     4261 tggtggacgg cgggtggaga tcctttggca atgccgtggc gctgggaccc gttgcaacaa
     4321 tccaggccga gcgcgctgag tcgccaaaac ttagcggcga gctttcgcgt gcgaaagtca
     4381 cgatgccacc gcctgccggc gttgatggag aatgcggtag taggtagcgg tggagatgcg
     4441 atgacggcgg cagatgtcct tgcgcttcgc gccctgctct atctcgctga tgatggcggc
     4501 gatgctgtag cgcgcgcgct gccggaaata gaggacttgg cctccgaact catgctggac
     4561 cgattcgatc acctggtcgg cgacccgggt tgcaatctgc tccgactcgc cggtggcctg
     4621 gcgcaccagg tgcgtgatgc gcgagcggag caccgcgaga tgatcctgcc ggtagcgagt
     4681 catagcccgc gccttgaacc gaagccttca aagcgagtgc cggcgacagg cgatgttcca
     4741 cgggggacag ttgcaaccgt ggcaggcgca tccccctggc gcggatcttc cagcagctcg
     4801 cgctggcgca ccttttcctc gacggccacc cactgggatt ccgtccaggt atggacgcgc
     4861 agcttgtgtg ctgcgtggag cgcatagact tcgagatcga gcggctcatt ccgcgcgccc
     4921 gctttcttgg tccacacggg gcgcccgcgc gggccgggca ccttgacctc gctggtgaga
     4981 ccggcgaagt agttgtcgcc gagatctgcg cagaaatgca tgcggcccgg gccgtcgccg
     5041 gccagcttga tgcggccgga gatgaggtcc ttggcttttt caacgccgac ccagtagagc
     5101 ttgagcccgt atttggccgc cttgtccgtg gcgtttgtgt cgatggattg ggacgggggc
     5161 gagaagattt ccttgtgcgg attggcgctg cccttgatgg ccatgaagcc gcggtgcttg
     5221 cggcggcgcg catagcggta gaccgcgtct tgagtcatgc cgtctgaact gtcgatcgac
     5281 acggcggaaa ttccgagggt gccgccgccg aggttgcgca ccggcttgtt caagatgggg
     5341 tctagcgcgt cccaagtggc gggctcaagt ggattgcccg gcaccacgcc ataggccacg
     5401 agccacgact ccatgccacg gccgaaggcc ttgacgacgt attcaagacg cggctcgccg
     5461 ctttcaccgc cgcgctgcac gtcaacggag gcgacgcaga tcaggccgcc ggccggggta
     5521 gtccattggg catagctttc cgcacgctcg cgcagctgct cgacttccgg tgcatcgctc
     5581 ttgagctgcc aggtttcgcc gagctggtta ttgacgaagc tgcgcatgtg cgagtcgtcg
     5641 ccgctcttga gcttgtgcga ggcctccagg aatttgcgca ccagcaattc catgggcgag
     5701 cccaccgaga cactcatcag ctcattgatg taaaagccgg cgatgccgtt gaatggcgcc
     5761 gtggcgcgcc agcccttgtc ggcacggcct gcggcggcgc ggaccgcagc gttcttttct
     5821 gcgtcggacc acaaggcccc acactcgtcc gcctcgcagc gatagcgggc cgtttccggg
     5881 cgatgcgtgc catagaccgg gtgcgacagc agcgcgtcgg actgccagtc cacctggtcc
     5941 cagcgcaggg tctgctcatg accgcaatgc gggcatgcga cgtagtagcg gcgcttgtct
     6001 gagcgctcca tgcctgcggc gactttcgag agcccctcga ccgttggcgt accgccaacg
     6061 ccgagcttgc cgtgctcgcc gtaagacttg acgcgctcac gacctagtgc gacggaatca
     6121 ccctggccgc gcaggtcctt gctggtgtcg tcaggttctt caacgaacac cagcggagcg
     6181 tccgtggatt tgacgttcgc cggcgagttc gtgccaacga gctggagata tccgccgggg
     6241 aaagccttgt agtcctgcgt gttgtccgga cgccgcgcgc cggtgttgat gcgggccgcg
     6301 atctcaggcg tggccctggc ttccggctcg accttttccc gcatgaagcg cttgccggag
     6361 ccatccttcg gaaacaggac gaggatggga ctcggctgca gatggacgcg cttgaaaatg
     6421 acgttaagcg tgacgccctg ggtccacccg aattgcgcgc acttctgccc ccagacctcc
     6481 cgtacatatg gatcgtcgaa tgcatcgagg atgccttcga gcgcgggctg gttgtggaac
     6541 aggaattttc ccggcagcag gcctccggcg agacggcgat agcgatcggt ccattgcgcg
     6601 gtgctgagcc gctctttcgg gaagaaacgg cggcgtgcgc ggcgcagaga gtcaatcgtg
     6661 cggccggaca gttgcgccat gatgctcatg cagctgcgct ctcgctgccc ggcgcaattg
     6721 gcagatcgct atccgaagcg agccggctga gcagttgtgt gaccgcatcg tcgatcacgc
     6781 ggaccatctg ctgcgtgcgc tctggcggaa ccagatcgcg catgatcgac ttgagggccg
     6841 agcccgcctc gacgatggcc tggcggatgt gcgtgacctg cagctctagc gcagcgtcga
     6901 gttcgggtgc agggatcagc tcgcgctgcg cttccatgcg cttggtgcgg atggtgatgg
     6961 cctcttccgc agccttccag ttacgtgggt cgcgcccctt caacacctcc agcagcgcat
     7021 cacggtcgct cagctgcagc cattccggct caggagcctt cggggttggc gccgcccccg
     7081 cccccggggt aggtgcaatt ctcgcggctg aaaattctgg ggcttcctgc tccggttcaa
     7141 cgccagcaag cagtgacggc tgcgcgccgg acagagcgcg gtcgatgcgg gagaccaggt
     7201 cggcgccatc accgcgggtg aatcccttgg cgcgctcggc catggccctg atgcgtagcc
     7261 aggccaggac ctgcgccagg ctgtatcggt gcggcttgcc ttgatcggca tgttcggcaa
     7321 tgggacaggg cggctcttcg cggatccact ggcgcacggc gtcggccgac caggtgccga
     7381 cgccgatctt ggcgagctcg gccaccagct gtcgaccggt gagagcctgg tcgatcatcg
     7441 ggcgcgcctc gcgtagccgt tcacggccag cgccggcttc acgcgccgat aacggcacca
     7501 ttcacggcca gcccgtgaac ccatccggca attcacacgg ctacgacaaa accgcgcctc
     7561 tgcgtacccg cgtgggaggt ggccagggag gacccatgag gccgggagtg ccaggcgcgc
     7621 gccgcccgct ttcgcgcgcg aaaagtgagc gtgaaaggcc agttcgcacc cttcacctac
     7681 cccttcacat tctttttccc tcgcatcttg atgtgttgtt aagggtgtta agggtgttaa
     7741 gggtccgcct tgtattaaag ggcggacaga atatgcacca gacgaatgca gatattgtct
     7801 cgcgcgcgca cgtgcgtggg cgcacccttc acacccttca cacgtataac aagccttggc
     7861 tgtgaacgag ttagcgcgtg aagggcgcat gcgttcgcct ttcacaccct taactgccgt
     7921 gctcattctt ccactcctca aactcgcgcc ggaatctctc catgccatcg ctgagccacg
     7981 cagcctcaga cttctcgttt tgagggggcg ggttgatacc gcgcggcaga tagaacgtgc
     8041 cctgcttttt gctttggccc accaggtacc acttgaccgc cgccggtaca tctttcttgg
     8101 cgcgcgccgc gaacttgttt tccggcttgg ggaatcgctc accgttctca cggcaccagt
     8161 acaggtaggc gtggaatagc tgctgcgtct tgcaaggccc ataggggacg ggtaacgcac
     8221 ccgcgatcca gtcgcgcagg aagcgctggt tgctttccat gctggcgtcg atcaagtcct
     8281 gcttggcagc agtcatcggc ggctttgtat gcggcccgaa gtcttcgagg tcgagctgca
     8341 gcaggcgatg gtagagcgca tccactgcgc tctcaggcat ggcatcagcg ccgaggccgc
     8401 ggtagtaatc gtagccgtcg ggatgcggcg gcggcgtcca gatgaccagg aagcggcggt
     8461 cgccagggtc aatcgagagc ggctgcagct cgttgctcag gaagaccagg ttcgcgaagt
     8521 tgatctcatc gcgcccgttc atgaacttgc gctcgacacg cagcgtgtcg tcgctgatca
     8581 tcgttttcag cctgcccttc tgctgaacct gttcctgccg gtgcagcacc tcgttgccca
     8641 cgatgaaaag cttggcgctg atccagtcgc accactgctg ctcaagctgc gtttgcgtga
     8701 tctggatgcc gtagcgccca aagatgcggc gcacggcatc ccagatcgtg ttcttaccgc
     8761 tgccctcggg gccatgcatg atgatgctgg tgcgcatctt cgtgcccgga tagcgcagcg
     8821 gataggcaat ccagcgcagc acccagtcgg tgattgcgtc gctctcgcca cagcagtaat
     8881 gcactacctt catccagcga tcgacgagcg cttcgtcggc cggcgtgccc ttcttcggaa
     8941 tgcgcggcca tccgccccac aggttgcaga tcgacggatc gtcgacgtct cgcttggggt
     9001 cgaagatgac ctgttccttg ttcaccacct cgcgcttcgg gtgatcgccc cagtcgcgta
     9061 cggccttcag gccggcgaag gcgcgcaggg cgcccagcgt catctcgatg cgtttctcgc
     9121 gatcgaacac cgcctcttcg ctgtagatca gcgcgtagcg cggcagcatc cattccacct
     9181 tcgtggaaaa gggcggcggc tcgcgcttgt ctttcttacc ggtctttttc ggcgcgcccg
     9241 gcaacatgcg cacacggcgt ttgtcgttct ccaaccaccc gctcgccgca gccttgccga
     9301 caagtgcggt gaaggcagag aagctcatct ccgagcccag ctcgcagtcc cacacgttct
     9361 tggtgccgta aatcaactca tagcgctgca gcaacgcttc cggggaattc tgcggaggtg
     9421 ccggcgcagg cgcagcggca tctgccggat caccaggggc cgggactctc tcaggccgcg
     9481 ccggcgcgcg cgctgcacgc ttgcgctgca cgctcggcgc acgccgtggg tcccagccat
     9541 gcttttttgc ctcggcgaat accaccttgt ggtcggtctg ctcaggcttg aacgacttcc
     9601 acttgtcgag cgccgcctgc gggtcatatt tcgccgattt ggaggaccac tccagccaca
     9661 acggcatgcc ggcgttgccc agcgtggcca gcgccattcc gatgctgatc cacgtgccgt
     9721 agtcgtccga gccgatgctg cgcaaggcat cgcgcaagtc atcggccaca tcatctgccg
     9781 tgctttgagg cagcggcgcc ggcgcagctt tcgcggcgga agctgccggc agttccggcg
     9841 ccaatggctt ctccagctcg aaagccagca caagcgcagc gatgtcatag ggcggcaatt
     9901 catgcagcgc atgaaggcgc gacacgaacg gttcatgctt ccggtgaatg aagcccggca
     9961 cgcgcatcac gcgcggcaga tcatgcacgc tcttgtccgc ctggaagcgc gcaatcagcc
    10021 cgcgctggta ctggcggaaa tccttccgcg ggcagtcgcg caccagccag taggcatgcc
    10081 agcggccagg cgaagactcc accacgatct gcggcagcag cggcgcctgc atcaccgggt
    10141 caagcggcgc gccatccaga tccacgaaca gcgcacgcac gcgggtgatg ttgcgcgctt
    10201 ccctgccctc gccatcggtt gcattcaccg tgacgaaaac gccggcgccc tggtcgttca
    10261 tccgcgacag ccaacgccat cgctcgtcga gcgtgccgtg cacgatctgc ccgagcgact
    10321 tgcgcttgga cggcgagtcg tcaaaggtct ggaaagtgaa gctcgcccca gcggcatcca
    10381 gcgcgcccag aaagacgcgc gcatccgcaa ggttccgctc cggcgacttg gctagcggtt
    10441 cggtcaagcg cgtctccgct gcgaatcgcg cagctcgttg agctcgccgc aatcgatgca
    10501 atgatcggca agcggcaccg ccttcaggcg cgcgggcgga atcggttcac cgcagcggcg
    10561 gcagcatcgg cggcgcagct tctgcggcgg cagtgtcgtc acccgcgccc tggcgttcag
    10621 atcctccagc tcggccagct gccgttcctg cacggcatcc atgtagtctg gactcatccc
    10681 ctttcccctc cctttcgttg tcgtcgtcgc tactcgctgc ccgggcgcac cgactgttcg
    10741 atacgcgcga tcaagccctc gcctgcggct agcaagccgc gcagttcgcg catggccagc
    10801 tcggcatgcg gcgcatcggc ctggtcgagc cggccgtcgt ccagcatcgg ccccagcgca
    10861 tcgagcacgc gggcgaactc cgacgaaagc ctgctcaggc gctgcatgtc gctgatctgc
    10921 gtattggccg gcaccggcac gtccagcagt ccgtaacgca acgccaggtc acgcacgcac
    10981 tcactgcgat acggctcagg aaaggcaaaa accaccgact cttcgaggtc cgatggcatg
    11041 cgggtggtgc cgtccatcaa gcggccgatg tgctgcccgt tgaccttggc gcgccggaac
    11101 acatcggcgt cggcggagaa atcaaagctc cgcgccggct ccggcacgcg cgcctggtat
    11161 tgctccagca ccagctgggc aaaggtttcc ttgctcatgc gcgtcttgcg catcaccagg
    11221 tcaaaaaaac gcgacacggt ctccgagcgc gtttcgtcca gccccacccg ctgtgcgctg
    11281 ttcttcattg gcaccccggc acgcggcgca tatggtgaaa aagcagcggc gtcgttgaag
    11341 acgccgcatt cgaatgacgg cctcgacggc cgctgggagt gacgacatgg gaaacgagcg
    11401 actgctggcc gcgattgaac gcgccctcaa tgctggatcc gtgcgccggg gtcagaacgc
    11461 acttttggtg gagcagatcg ctgcgtgcat catgaaggtg ggccatatca cgccgccttc
    11521 cggtcggaat ccgacgccgc cgcggtttcg tctggccaca ggtcgggacg cagttgcccc
    11581 ttgcgcagat cgcgcccggt tttttcctcg atcgcaatgg cgcgctccgc cgagatgcgg
    11641 cgtatgcctc caacccattg cgccaccatg ctctgggtca ccccgacaag ctcggcaaac
    11701 tgagcctgcg tcaggttttt ctcgcggagg tattcggcaa tcgccatgcg ccgattatta
    11761 gcattgctgt tgttttgtca acagcaacgc tgtttgcatg tatataagca ctgctaataa
    11821 tgcggatatg ttgaaacgta agccgctaac acaggacgag ctcgatgcag ccgaaaatct
    11881 gcggcagctt ttcaccgagg ccaaagcggc cgacaaaacg ctgacgcagg aacgtctggc
    11941 ctttcaattc ggctggaaaa cccagagcgc ggtaagccag tacctgcgtg gcgtcatacc
    12001 gctaaattcc gacgccgccc tgaaattcgc cgactttttc agggtcgcgc cctcgcaaat
    12061 tcatcccatg ttttcccgcc tcgatcgagc agcaattgcg gagccgaaaa ccaagtacgg
    12121 aacggaatta catctgcggg agatccctgt cgttggccgg ggtaagggcg gcgaagacgg
    12181 atttttcgag gaagaggatt ttcctgtcgg ccacggcaat gaagtcattc tccatgcaag
    12241 cgccgaccac aacgcgttct gcgttagggt cgagggtgac tcgatgtcac cgcgttacag
    12301 gcatggcgaa tacgtcctgg tggagccgaa tcagccgggc caggcaggcg acgacgtcta
    12361 tgtcgttctc agaaatggtc gcaagctgat caaggaactc gcctatatgc gcgacagcga
    12421 gatcaagctt atctccgtga acgcaacaga ccatccgcct cgcacctttc cgctttcgga
    12481 ggtagattcc atctatcgca tcggaggaag catcaggcgc ggaagcggat tcgtccgatc
    12541 ctgaccgagc tggaccgggc taagggattt gccatggcac ccaaactgat gatctgcacc
    12601 agctgctacg aattcacttt cggcactacc aagacgcgtg ggagtttcct ggtcgaggtg
    12661 gtgctttggc tttgcttcct gctgcccggg gttatctaca gcctgtggcg gctcagcacc
    12721 cgccacgacg cgtgcgataa atgcggtggc cccatcattc ctgccgacac cacccgcggc
    12781 cgagcacttg ccaagcaata ccacagcgac ggcatggcca cgccgcgcgc ggcctgatca
    12841 ggccatcatc acttccagcc aacggctggc gcgctgctct tctgggtctg cattgccgtc
    12901 tgccgctacg agttcagtca cagcattgcg cagcagtgtg cggaattccg ccggacccga
    12961 ccgcagctgc ttgatgccga caagaaactc accctggtcg cagctcagcc gctgaagctg
    13021 gccgtgcacg tctgaccacg ggaattccac accgagattg cgctcggcat tgcggagaaa
    13081 cttggcgatc acacctcgct ccgtcatgtc catccttcca tccgagcgag cgacataaac
    13141 catgcaaaga acggcgcgtc gggccagcct cagtcgattc gccggcgatg caatagcagg
    13201 tagcacctgc ggttcagccg gtctttcggt ggcggctgca ggttccgctt cgaactgctc
    13261 atggtccggc caggagcggt actgctgcca caaccacggc gccggcggac ggtgactttc
    13321 gccagtgttc agatccacca gctcttgcat tcggctggct agaaatgtcc ttagggcatc
    13381 gcgctcatgg caccacgcga tcattgccag gtcatcgccg ccgccccatg ggcgcaggca
    13441 tcgggtcgtg acgcgccgct ttgactcgtc acctttcgca tcccggtagt agagatacat
    13501 cgtctgggtg aatggaacac ccgagccgcc cgcgtcgacg atcccctcgt ccgcggcgtt
    13561 gctgctgacc ttcgtcgtga gcccggtcag gagctttgga accctttgct ttggcaccca
    13621 gctcatagat ctccccttct gaagtgcgct gcatatcttc gcgtgagaca gatgaagagc
    13681 gcacctcatg ggaattttta ttagcattgc tgttgacacg acttagaagc attgctaata
    13741 attcggccca tctcatcgga gtgggccatg caacaaaccc tgtttcatac ctgcggcgcc
    13801 cgggttgacg tcaacgaggt catcgagggc cgcagcaacc gcttcgtctt cctcgacgcg
    13861 ctgaccggcg acggcctgga ccgctgcccg cgctgcagcc gcgagctgca catccatgcg
    13921 ttccgcccgc aggcgccgct gcccaagctt tcgcgcgcga aagccgcgcg ccccgccggc
    13981 gccaagcgcg aggcccgccg cctgatcgcc aaggcgcggc gcatccgcac gcagcggagc
    14041 gcggcatgag cgccgctccg ctgcacacgc cggagccgtg gcccgatagc gcgagtgccg
    14101 actacgctcg gcagcgcctc tgtcagcttc aggaattcgc gcatcaacag gtcggcatgc
    14161 agcattcgtg cgactgctgc tgcgggcatg gcggattctg gagagataag aacagccggt
    14221 acggcggcac gttcgccgag ggctatcgca acgatggcgc ggcgatccaa tggcttgaga
    14281 tagcggccag agcctgtgct ggcatgcccg atccggctgc ggagatagag cgcctgctgg
    14341 gcgaacatca cacggccagc ggcgcatatg ccctgctgga gcatgaggcc gaggccctgc
    14401 gcgccagcaa cgccgaactg ctggcggcgc tgaaagacgc cagggagacc atccgcacat
    14461 ggcatgggcc agtggcatgg gagatctacg acaaagaatc tcctgaaatg aagcggctga
    14521 acagtgccat tgccaaagcc gagggccgcg catgaacgtc ccgctcaacc agctgtacgt
    14581 catcatcgac ctcaagcacg gcgtgactga aagcgaactg gccgaagccg tgaagctgct
    14641 cgcggccaag acgcacggcg atatcaacgc cgcatatcgc gtggtcaccc cgccgcgccg
    14701 cacctggtgg gagcgcttca cccagcttgt cacgggcgcg cccgtctacg cgcttcgcca
    14761 ggtgccgcta tgaccccgca gccgcgtctc gcgcttctcg ccagccacca gggcgaggcc
    14821 tgcgcgctca tcctgcaaag cctgcagacc acgcacgccg acgtgctgcg cttcgacgac
    14881 atcagccgcg cagagacgcg cgatggcgtt gccaccgtcg tcggcgactt cacccacctg
    14941 ccttccatgc gccgctacgt catcgcgata gcggcagtac cgcttgatta aagggagctg
    15001 ctcatgaacc acctgaaagt cgtaaccacc cagcggcagc ctgaactgga tttccaggct
    15061 caggcccagg agctggtcgc acgccgcaac gaacacctgt gccacgcgct ttcgctctat
    15121 gagcgcgcgg aagaggaata caagttcgcg caaaaggcct tggaccacgc gcgccagcgc
    15181 cgcgacgagg cgcgcgccca gctcacccac ctcgtcggcc gcgacgaaaa gctgctggtg
    15241 ggcaatgtcc tgctcacccg ccatgccaat ggcgggctgc acatcgaatc cgtcagcgtc
    15301 gtcggcctgg tctaggagaa ctgcaatgcc cgaggcatca actttcgccc gcgaaaccgc
    15361 cgccatgccg gtggaacaga tcagcctgga cctgatcagc gtccaggacc agatccgcaa
    15421 gaacttcgac cccgacaagc tggagcagct gggccagagc atcaagcagc acggcctggt
    15481 gcagcccatc gtggtggcgc cggagaacaa cggcaccggc atgcgcctgc tcatgggcga
    15541 gcgccgcctg cgcgccgcca aggccgcggg cctcaccacc ctgcccacca tcgttcggca
    15601 tgacctcgcc ggccaggacg tgcgcgtgct gcaggccatt gaaaacctgc agcgcgaaga
    15661 cctgaccatg gccgaggaat gcgccggcgt caccgcgctg gtcgagtcca tcggcttcga
    15721 ggccacctgc cagcagctgt gcaagagcga aagctgggtg agcaagcgct ccaccatcaa
    15781 gcagctgccg gcgcccatca aagacctggt cgagcaaggc aaggtcaaag atcttgaagt
    15841 ggtgcacctg ctcggccagc tggccaagca caacagcacc aaggcctttg aactggcgcg
    15901 catggcgcgc gtcgtggatg acgcaggcaa cctgccggcg caccagcagc ccacgcgcga
    15961 aaacatctcc gcgctggtcc gcgccgagcg cgaaacggcc gagcgcaagc agcaggaaaa
    16021 gatcgagcgc gacgccgcca agaacgaccc cgccgccatc aaggccaaga agcgcgacgc
    16081 tagcgccaag gaagagcgca agcagctgct ggccaagctg cagcagctca aggacgccgg
    16141 caccgcgctg gaagacgaaa ccatggaagc gctgggcaag gcgctgggca tcaaggtgcc
    16201 gccgcgcggc gagttcggcg gccgcggcca ttgcgacgtg atggtggcca tggactatct
    16261 gcaggagcac atgaagctgc cggccagcgt cgacggcgcc ggcttcacct tccgcgccga
    16321 tggatccgtg tcgctgctca agagcgtgtg caagctcaca gcgcacgcgc agaagctggc
    16381 catcaccatc cgcgaggtca gcgtggacca ggccgagaaa atcgccgcca tcgtcggcga
    16441 gaaggccgta accgtcggcc tggtcaagcg cgtcaacggc aaggaactgc gcagctacat
    16501 cgagcgcgcg cgggccgcca agcacgaagc caagacggaa aagcgcgagg ccgcgaagga
    16561 agggccggaa taccagctgg ccaagctgcg cgaattcctg cgcaagcacg tcaccgtcat
    16621 cggcggcaag gaagctcgcc ttaatacggt gaccgccaag gcaatgtacg gggcctatgc
    16681 cgcctactgc caggccaaca aggttgagcc gctgtcgctc aacgacaacg cctacggtgc
    16741 cgcaatcctc gcccatgccg gcggcggcat cgtcaagaag cgcacgaata cgggctatgt
    16801 gtatgtcggg tgctcgctgg catgagcgcc gtgactgctg aatcgaacct gcaactggtc
    16861 gcgcaggcgt cggaactgat gcgcctggcc tttgaaatcg ccaacgattc cgctcagagc
    16921 gatgtggagt gcatgtgcca catccacagc ggcaacgccg atgaaggctg gtggtacgaa
    16981 ctgggcaagc cgtcgcccga gc
//
LOCUS       DASZZC010000568         7838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_239594,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000568 DASZZC010000000
VERSION     DASZZC010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239594"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VHE37_12105"
     CDS             <1..425
                     /locus_tag="VHE37_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HVT36326.1"
                     /translation="FRNAFLRELAIEFKPVEPWNPLAPVARPPQQAIWFRAAGKLPDD
                     PMLHRCVLAYASDFNLLSTALLPHGVTWFSRDMVVASIDHAIWFHRDARADDWLLYVM
                     DSPSSQSARGLSRASIFARDGRLVASIAQENLMRRVRD"
     gene            complement(415..1872)
                     /locus_tag="VHE37_12110"
     CDS             complement(415..1872)
                     /locus_tag="VHE37_12110"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HVT36327.1"
                     /translation="MALKLKDASLLKQACYINGAWVQGSANIAVRDPGTGEHFASVPK
                     LGGADARRAIEAAYHAGPAWAALTARERGLVLRKWADLMMANQDDLALLLTSEQGKPL
                     DEARGEIAYSASFLEWFGEEGKRIYGDTIPAYTRDKRVTVLKQPVGVCAAITPWNFPS
                     AMITRKAGPALAAGCTMVVKPASQTPLSALALAELAHRAGVPAGVFSVVTGDAGAIGD
                     ELATSELVRKLSFTGSTEIGRQLMAKCAGTIKKVSLELGGNNPFIVFDDADVDAAVDG
                     CIASKYRNTGQTCVCANRVLVQDGVYDVFAKKLAEKVAQLKVGYGTEAGVKQGPLINP
                     PAVEKVEEHIADAKEKGAKVLTGGARHELGRTYFQPTVLSGCNTDMKIAHEETFGPVA
                     PLFRFKTEQEGVELANATEFGLVAYFYARDVARVTRVGEKLEAGMVGINTGLISDSSV
                     PFGGVKQSGLGREGSRYGVDEYIEVKYLCLGGISP"
     gene            2018..2428
                     /locus_tag="VHE37_12115"
     CDS             2018..2428
                     /locus_tag="VHE37_12115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT36328.1"
                     /translation="MTATDDYRYRLSIGTRWKDNDVYGHVNNVEYYSYFDTVINTFLI
                     REGGLDIHQGAVIGLCVESHCEFKAPIAFPDAVEAALRVSKIGRSSVRYEVGIYTRGA
                     LAAQGHFVHVFVDRATRRPADIPAPLRAALETLA"
     gene            2425..3549
                     /locus_tag="VHE37_12120"
     CDS             2425..3549
                     /locus_tag="VHE37_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HVT36329.1"
                     /translation="MKIRAAVLRSMGHARPYVDSKPVTIEQIELAAPGPGEMLVKMLA
                     AGICHSDLSVVDGSRPRVMPMVLGHEACGEVVECGGGVADFAPGDRVVFSFVPVCGHC
                     PQCAQGRAALCEPGAKSNVAGTLLTGARRVRDASVELNHHLGVSGFAEYAVVSPRSAV
                     KIDRDLAPEIAALFGCAVLTGVGAAVNTARIQPGQSVAVIGLGGVGLAALLGARACGA
                     QPIVAVDVVQSKLELARALGATHVIDARAGDVAAMVKDVTGGGADVVLETAGGAQTLA
                     QAYAATRRGGTTVTMSLPDPKVPLQIPAVSLVVEERTLKGSYMGSAVPSRDVPRYIAL
                     YRAGRLPVEQLLTHTMKLDELNLGFDRLARGEAVRQVILF"
     gene            complement(3702..5660)
                     /locus_tag="VHE37_12125"
     CDS             complement(3702..5660)
                     /locus_tag="VHE37_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVT36330.1"
                     /translation="MKSNKFLAAGIVVGAALGLQACGSSVPGSVGAGEPSSSGGAVKS
                     GWTTLGTREASSKATTVSAPNASQWTDYNPPAQYPGVVTTKNVFITMPDGVQLAANVT
                     QPADASGNAAPGQFPTVLVQTGYNKDAGIQETGGGADTTLVEHGYVQVTVDERGTGRS
                     QGEWHSFDAVEQADYPVVVNWVTQQPWSNGKVGLQGASLLAITALLTAAQQPPGVVAM
                     FAEIPMGDSYRDIVFQGGELNAGFIPLWLGLVTGLSVLDPTIFTAPQESLTAELSHVE
                     GAVVGFQVPQVLNSVVGDPSTVYDGDFWKVRSPLDHATNINIPTFVIGGLHCIFQRGE
                     PLLYETIKQHAPSKLLLGPWTHVQMGQGLPVDGVPAIEHIELQWFDQYLKGMDVGADK
                     QPNVTQYVYGYDHYVTSTDWPNPDAQAQRYYLHGDKSVSTQAPAADEATNTVLQEPAN
                     GLCSESSDQWTAGLLGMVDVTKILPCFTDDTLTEALEAKFDTAPLTDDLYFNGPIEAD
                     VWVSTTAQDAPVVVRVDDVDASGSAFPLTNGIMQASMSAVDASKARYLDGVMIEPWHP
                     FLQSGVQAIASGTPVLVQVEVFPTSAMIAKGHKLRISVGASDLPHGLPPAPALVKEAA
                     GVMTVYSDAAHPSLVVLPVMPAGALPPS"
     gene            complement(5698..7569)
                     /locus_tag="VHE37_12130"
     CDS             complement(5698..7569)
                     /locus_tag="VHE37_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVT36331.1"
                     /translation="MKFQLMAGALLAVLLAGCGNSMPGATSKDSGGGASDSTGSTTNP
                     VKADRDGTIYRQAIDVTATGDNQVFQVFEPTHLKVGQTYPLVLQGHGYGGSRETAAPD
                     GSFIKKLMDAGYYVISIDERGFGESSGQVRVMDPEFEGADLTAILDWAENLEGLRRGG
                     DGQMVVGSYGGSYGGMYQFALMGSDPKHRLHVLAPDITPHDLTYALDENNVVKSGWGL
                     VLIAGGEAGAFEGDPSSALQQLLTRIQAGEVTRQDPILYEILLDASLNNAFTDGGKAF
                     FEYHSVNYFCAGQPAAAQGGFVVGTPDQLLVAPTAFPRADVLLTQGFRDTLFNFNNAY
                     DNYQCLKQLGGDVRLYTHQTGHILPVSASTVPLPPGSNLETALDPFYTAINLPAFQDA
                     GGSRSCGKLVLDDLQFAWFQEKLQHIAGAVDAATNSGRDICLSVAEGDSIQVPSVKIG
                     GTPFRLDASTPQFNSVLSVGGAALGNGAREALLATQPIYTAAADTVVAGIPTLSVDIE
                     GLTGAEMTGCATPLVQAACDPIFYIGLGHQHAGSNLWDLVDDQITPVRGFGQHDLQMN
                     GIAERIPAGDQLAVLIYGFHLQYPVTWSRDLLVPAATISGTISLPVLDKSEIVRDGG"
BASE COUNT         1245 a         2644 c         2586 g         1363 t
ORIGIN      
        1 acttccgcaa cgccttcctg cgcgagctgg ccatcgagtt caagccggtg gagccgtgga
       61 acccgctggc gccggtcgcg cgtccgccgc agcaagccat ctggttccgc gccgccggca
      121 aattgcccga tgacccgatg ctgcaccgct gcgtgctggc ctatgcgtcg gacttcaacc
      181 tgctgtccac cgcgctgctg ccgcacggcg tcacctggtt ctcgcgcgac atggtggtgg
      241 cctcgatcga ccacgccatc tggttccacc gcgacgcgcg cgccgacgac tggctgctct
      301 acgtgatgga ttcgccgtca tcgcagtcgg cgcgcggcct gtcgcgggcc agcatctttg
      361 cgcgcgatgg acgcctggtg gcgagcatcg cgcaggaaaa cctgatgcgt cgggtcaggg
      421 actgatgcca cccaggcaca ggtacttcac ctcgatatat tcatcgacgc catagcgtga
      481 accttcgcgg ccgagacccg attgcttgac gccgccgaat ggcaccgatg aatccgagat
      541 caggccggtg ttgatgccga ccatgccggc ctcgagtttt tcgcccacgc gcgtcacccg
      601 cgcgacatcg cgcgcgtaga agtacgccac caggccgaac tccgtggcgt tggccagctc
      661 gacgccttcc tgctcagtct tgaagcggaa cagcggcgcc acggggccga aggtttcctc
      721 gtgcgcgatc ttcatgtcgg tgttgcagcc ggacagcacg gtcggctgga aatacgtgcg
      781 ccccagttca tggcgtgcgc cgccggtgag cactttcgcg cctttctcct tcgcgtcggc
      841 gatgtgctct tccacctttt ccaccgcggg cggattgatc agcggcccct gcttcacgcc
      901 ggcctcggtg ccgtatccga ccttcagttg tgcgactttt tccgccagct tctttgcaaa
      961 cacgtcgtag acaccgtcct gcaccagcac gcggttggcg cacacgcagg tctgcccggt
     1021 gttgcggtac ttcgacgcga tgcagccgtc gaccgcggca tcgacgtcgg catcgtcgaa
     1081 cacgatgaag ggattgttgc cgcccagttc cagcgacact ttcttgatgg tgccggcgca
     1141 cttcgccatc agctggcggc cgatttccgt cgaaccggtg aacgacagct tgcgcaccag
     1201 ttcgctggtc gccagttcat caccgatcgc gccggcatcg ccggttacca cgctgaacac
     1261 gccggcaggc acacccgcac ggtgcgcaag ttccgccagc gccagcgccg acagcggcgt
     1321 ctgcgaggcc ggcttgacca ccatggtgca gccggcggcg agcgcaggcc cggccttgcg
     1381 cgtgatcatc gcgctgggaa aattccacgg cgtgatcgcc gcgcacacgc ccaccggctg
     1441 cttgagcacg gtgacgcgct tgtcgcgcgt ataagccgga atagtatcgc cgtagatgcg
     1501 cttgccttcc tcgccgaacc actcgaggaa cgaggcgctg taggcaatct cgccgcgcgc
     1561 ttcgtccagc ggcttgccct gctcgctggt cagcagcagc gcgagatcat cctgattcgc
     1621 catcatcagg tcggcccact tgcgcaggac cagcccgcgc tcgcgcgcgg tgagcgccgc
     1681 ccacgccgga cctgcgtggt acgcggcctc gatggcgcgg cgtgcatcgg ccccgccgag
     1741 cttcggcacg ctggcaaaat gctcgccggt gcccggatcg cgcacggcga tgttggcact
     1801 gccctgcacc caggcgccgt tgatgtagca cgcctgcttg agcagggaag catctttcag
     1861 tttgagcgcc atggtcagtt ctccttggcc gtcattcccg cgcaggcggg aatccagttc
     1921 ttcttcaacg ctaaaactgg atgcccgcct ctgcggacat gacgagtgtc ggtctacgca
     1981 tcggataaga tcgcgcaaat tttgtcaggt tctgtgcatg acggcgaccg acgactaccg
     2041 ctaccggctc agcattggca cgcgatggaa ggacaatgac gtctacggac acgtcaacaa
     2101 cgtcgagtac tacagttact tcgacacggt gatcaacacc ttcctcatcc gcgagggcgg
     2161 gctcgacatc caccagggcg cggtgatcgg cctgtgcgtg gaatcgcact gcgagttcaa
     2221 ggcgccgatc gcctttcccg atgcggtcga ggcggcgctg cgcgtgagca agatcgggcg
     2281 cagcagcgtg cgctacgaag tcggcatcta cacccggggg gcgctcgctg cgcagggcca
     2341 tttcgtgcac gtgtttgtcg accgcgccac gcgccgcccg gccgacattc ccgcgccgct
     2401 gcgcgcggca ctggagacac tcgcatgaag atccgcgccg cagtgctgcg ctcgatggga
     2461 cacgccaggc cctacgtcga ttcgaagccc gtcaccatcg agcagatcga gctcgccgcg
     2521 cccggcccgg gcgagatgct ggtgaagatg ctggccgccg gcatctgcca ttccgacctt
     2581 tccgtggtcg acggctcgcg tccgcgcgtg atgccgatgg tgctgggcca cgaggcctgc
     2641 ggcgaagtgg tcgagtgcgg cggcggcgtg gccgactttg caccgggtga ccgcgtggtg
     2701 ttctccttcg tgccggtgtg cgggcattgc ccgcagtgcg cgcagggccg cgcggcgctg
     2761 tgcgagcccg gggcgaaatc gaatgtggcc ggcacgctcc tcaccggcgc gcgccgcgtg
     2821 cgcgatgcca gcgtggagct gaatcatcat ctcggcgttt cgggattcgc cgagtacgcg
     2881 gtggtgtcgc cgcgctcggc ggtgaagatc gaccgggatc tcgcgccgga gatcgccgcg
     2941 ctgttcggct gcgccgtgct caccggcgtg ggcgccgcgg tgaacaccgc gcgcatccag
     3001 cccggccaga gcgtggcggt gatcggcctg ggcggcgtcg gcctcgccgc gctgcttggc
     3061 gccagggcct gcggcgcgca gccgatcgtc gcggtcgacg tggtgcagtc caagctcgaa
     3121 ctggcgcgcg cgctcggcgc cacgcacgtg atcgatgcgc gggccggcga cgtcgctgcc
     3181 atggtgaagg acgtcaccgg tggtggcgcc gacgtggtgc tggaaaccgc cggcggtgcg
     3241 cagacgctgg cgcaggccta cgctgccacg cggcgcggcg gcaccaccgt gaccatgagc
     3301 ctgcccgatc cgaaagtgcc gctgcagatt cccgcggtga gcctggtcgt cgaggagcgc
     3361 acgctcaagg gttcatacat gggctcggcg gtgccgtcac gcgacgtgcc gcgctacatc
     3421 gcgctgtacc gcgcgggcag gctgccggtg gagcagctgc tcacgcacac catgaagctg
     3481 gacgagctca atctcgggtt cgaccggctg gcgcgtggcg aggcggtgcg gcaggtgatc
     3541 ctgttctagc gtagtgaact gcaaaaacct tgatcggata atcctgtcat tcccgcgaag
     3601 gcggtaatcc agtcttgctt ctgtgatgga tcaaaaacaa aactgggttc cccgccttcg
     3661 cggggacgac gatttgtttt tttaacgacc tgctgactca cctaggaagg cggcaacgcg
     3721 cccgccggca ttaccggcaa caccaccagc gaaggatgcg cggcatcgct gtacaccgtc
     3781 atcacgccgg cggcttcctt caccagcgcc ggcgccggcg gcaggccgtg cggcaagtcg
     3841 ctggcaccca ccgagatgcg cagcttgtgc cccttggcga tcatcgcgct ggtcgggaat
     3901 acctcaacct gcaccagcac cggcgtgccc gacgcaatcg cctgcacgcc ggactgcagg
     3961 aagggatgcc acggctcgat catcacacca tcgaggtagc gcgccttcga tgcgtcaacg
     4021 gcgctcatcg aggcctgcat gatgccgttg gtcagcggga aggcgctgcc gctggcatcg
     4081 acgtcatcca cgcgcaccac caccggcgca tcctgcgcag tggtcgacac ccagacatcg
     4141 gcttcgatcg gaccgttgaa gtagaggtcg tcggtgagcg gcgccgtgtc gaacttcgcc
     4201 tccagcgcct cggtgagcgt gtcgtcggtg aaacacggca ggatcttggt gacgtcgacc
     4261 atgcccagca ggcccgcggt ccactggtcg gagctttccg agcacagtcc gttcgccggc
     4321 tcctgcagca cggtgttggt ggcctcgtcc gccgccggcg cctgcgtgct cacgctcttg
     4381 tcgccatgca ggtaatagcg ctgcgcctgc gcatcgggat tgggccagtc ggtcgacgtg
     4441 acgtaatggt cgtagccgta cacgtactgc gtcacgttgg gctgcttgtc ggcgcccaca
     4501 tccatgcctt tcaaatactg gtcaaaccat tggagttcga tgtgctcgat cgcgggcacg
     4561 ccgtccaccg gcaggccctg gcccatctgc acgtgggtcc acggcccgag cagcagcttg
     4621 gacggcgcgt gctgcttgat cgtttcgtac agcagcggct cgccgcgctg gaagatgcag
     4681 tgcaggccgc cgatgacgaa ggtggggatg ttgatgttgg tcgcatgatc cagcggcgag
     4741 cgcactttcc agaaatcgcc gtcgtaaacc gtcgacgggt cacccaccac ggaattcagc
     4801 acctgcggca cctggaaacc caccaccgcg ccttcaacgt gggacagctc cgccgtcagc
     4861 gattcctggg gggcggtaaa gatcgtggga tcgaggacgg acaggccggt gaccagtccg
     4921 agccacagtg ggatgaagcc ggcgttgagt tcgccgccct ggaacacgat gtcgcggtag
     4981 gagtcaccca tcgggatttc cgcgaacatc gccaccacgc ccggcggctg ctgcgcggcg
     5041 gtgagcagcg cggtaatggc cagcagcgat gcgccctgca gccccacctt gccgttgctc
     5101 cacggctgct gcgtcaccca gttcaccacc accgggtagt cggcctgctc gaccgcgtcg
     5161 aacgaatgcc attccccctg cgagcggccg gtgccgcgct cgtccacggt gacctgcacg
     5221 tagccgtgct cgaccagcgt ggtgtccgcg ccgccgccgg tttcctggat gccggcatcc
     5281 ttgttgtagc cggtctgcac gagcaccgtc gggaactggc ccggcgccgc attaccgctg
     5341 gcatcggccg gctgggtgac gttggccgcc agctgcacgc cgtcgggcat ggtgatgaac
     5401 acgttcttgg tggtgaccac gcccgggtat tgcgcaggcg ggttgtagtc ggtccattgg
     5461 ctcgcgttgg gcgcgctgac cgtcgtggcc ttgctcgaag cctcgcgcgt gcccagcgtg
     5521 gtccagccgg atttgaccgc gccaccggac gacgatggct ctcccgcgcc aacactgccg
     5581 ggcacggacg agccacaggc ctgcagaccg agcgccgcgc cgaccacgat gccggccgcc
     5641 aggaacttgt tcgacttcat gtatgcctcc agaaacgttc tacgaaacgc ccgcgcatca
     5701 gccaccgtcg cgcacgatct ccgacttgtc caggaccggc aggctgatgg tgccgctgat
     5761 ggtcgccgcc ggcaccagca ggtcgcgcga ccaggtcacc gggtactgca ggtggaagcc
     5821 gtagatcagc accgccagct ggtcgcccgc gggtatgcgc tcggcgatgc cattcatctg
     5881 caggtcatgc tggccaaatc cgcgcaccgg cgtgatctgg tcgtcgacca ggtcccacag
     5941 gttgctgccc gcatgctgat ggccgaggcc gatgtagaag atcggatcgc acgccgcctg
     6001 caccagcggc gtggcgcagc cggtcatctc cgcgccggtc aggccttcga tgtcgacgct
     6061 cagggtcggg atgccggcga cgaccgtatc ggcggcagcg gtgtagatcg gctgcgtggc
     6121 cagcagcgct tcgcgcgcgc cgttgcccag cgccgcgccg cccaccgaca gcacgctgtt
     6181 gaactgcggt gtcgaagcat cgagcctgaa cggcgtgccg ccgatcttca cgctgggcac
     6241 ctggatcgag tcgccctcgg ccaccgacag gcagatgtcg cgcccgctgt tggtggcggc
     6301 gtccaccgcg ccggcgatgt gctgcagctt ctcctggaac caggcgaact gcaggtcatc
     6361 cagcaccagc ttgccgcagc tgcgcgagcc acccgcatcc tggaacgccg gcaggttgat
     6421 ggcggtgtag aacggatcga gcgcggtttc caggttgctt cccggcggca gcggcaccgt
     6481 gctggccgac accggcagga tgtggccggt ctggtgcgtg tacaggcgca cgtcgccgcc
     6541 gagctgcttc aggcactggt agttgtcgta ggcattgttg aagttgaaca gcgtgtcgcg
     6601 gaagccctgc gtgagcagca cgtcggcgcg cgggaaggcg gtcggcgcta ccagcagctg
     6661 gtcgggcgtg ccgaccacga agccgccttg cgccgcggcc ggctgacccg cgcagaaata
     6721 attcacgctg tggtattcga agaaggcctt gccgccgtcg gtgaaggcat tgttgagcga
     6781 ggcgtcgagc aggatctcgt acaggatcgg gtcctgccgc gtgacttcac cggcctggat
     6841 gcgcgtgagc agctgctgca gcgcgctgga cggatcgccc tcgaacgcgc cggcttcacc
     6901 gccagcgatc agcaccaggc cccagcccga cttgaccacg ttgttctcgt ccagcgcgta
     6961 ggtcaggtca tgcggcgtga tgtccggcgc cagcacgtgc aggcggtgct tcggatcgct
     7021 gcccatcagc gcgaactggt acatgccgcc gtaggaaccg ccgtagctgc ccaccaccat
     7081 ctggccgtcg ccgccgcggc gcagtccttc caggttctcc gcccaatcaa gaatcgcggt
     7141 gagatcggcc ccttcgaact ccgggtccat cacgcgcacc tggccgctgg attcgccgaa
     7201 gccgcgctcg tcgatggaga tgacgtagta gccggcatcc atcagcttct tgatgaagct
     7261 gccgtcgggt gcggcggtct cgcggctgcc gccatagccg tggccctgca gcaccagcgg
     7321 ataggtctgg cccaccttca ggtgcgtggg ctcgaacacc tggaacacct ggttgtcgcc
     7381 ggtggcggtg acgtcaatgg cctggcggta gatggtgccg tcgcggtcgg ccttcaccgg
     7441 gttggtggtg ctgccggtgc tgtccgacgc accgccgccg ctgtccttgc tggtggcgcc
     7501 gggcatgctg ttgccgcaac ccgcaagcaa cactgccagc agcgcgccgg ccatcagctg
     7561 gaatttcatc ggcatctctc ctcgggcaaa gcgcgcatct gacggcgcgt tcaggctgcg
     7621 catcataagg ccgcgtgcag ccgtgcaaca gggagaaaaa cgatcagccg ctcagcggcc
     7681 gagcagcagg cgccagccga tgcaggccag tgtgccgatg gtgaggatgg agcggaactg
     7741 gaaatagccg tcgatcgagt ccagcgtgcg gtcgcgccac acgtcggcga ccagcagcag
     7801 cacgaacacg ccggccaggc cgtacaaggc aagatcga
//
LOCUS       DASZZC010000569         5531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_175480,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000569 DASZZC010000000
VERSION     DASZZC010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175480"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3086
                     /locus_tag="VHE37_12135"
     CDS             <1..3086
                     /locus_tag="VHE37_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36332.1"
                     /translation="DTFSLRGVYKSTDGATWTSASSGLPANPSIRNLAIRGTKLWVYL
                     GGQGLFRSTDGAASWQNVTTGLSGDLFGKVFAASTVLIASAGGVVYRSTDDGDTWAAT
                     TGYAVSKFGIEILSMAEDAAGALYLGDEHAGLSVSKDAGVTWAPSSNGLATSNVCIER
                     INSLLFKGGKIYAGQGEGGGVRVSSPIGAAAPGDSVACTTDKDIFPNNFSFTAQTNVK
                     PSTLVKSNIITVAGLGNGVSVPISVSGGEFAKSATCGAGFTTTTGHVVNGDQVCVRHT
                     SAATSETSVKTRLTIGDKSATFTSSTGLVFTGRTDEINTGFGSNGFASVASQTTASDV
                     KILADGSFVSAAATSGFPADIAITKFNYDGSVDTSFGVGGVAKFDAGGSESVVAVIAQ
                     SSGKLVAVANTRIGTPSATNKIVLVRFNADGSIDSGFGTSGMVTFDLNPNQSGNGENA
                     SSAIALSDGSILIGGGVAITGTGTHFFVVKFSADGVQDTSFGTANATLGSTFADVQVG
                     SSTVVGGALAVQSDGKVLMSGQADVGFNTIRGLVVRFTSAGVLDSGFGSSGKFASASA
                     IALPALLVLGDGSIMAAGQTTSSPFSPVMTRLTSSGALDTSFGSSGVLTLTLSGNSSS
                     GSLSSLLLLANGGIAFSGQAYQSDLGASALLFGILNSNGTLDTSFGNNHGYLVRRPVN
                     GSNVLGGRMAQRADGGVLFGGLYGSSLGAFVFGGTSGPPPDTTPNAFTFATKTAVSRN
                     VQVTSAAKAIKGINAPAPVTISGGTYSIGTTDCGNTYTSAAGMISNGQKICLRHTSSR
                     SPATKTSTIVTIGGVSGTFSSVTVDDVPAAFKFTDAAGVTPSSVVVSDTVTVSGIDAP
                     AAVSVKNGEYSIGISDCGNTFTSADGTISNGQMICVRHTAAAGNNKAINTVLIIGGVQ
                     DTFSSVTAKGTGTADVMPNFFHFPDVNNAAPSTLTASQALTIAGIANGSTAVVKVTGG
                     EYSIDGGAFTSADGTIGSGQTIAVRLTSSANASTPVSATLNISGLTDAFTVTTGK"
     gene            complement(3199..5064)
                     /locus_tag="VHE37_12140"
     CDS             complement(3199..5064)
                     /locus_tag="VHE37_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT36333.1"
                     /translation="MLLLTLRDLSLKIGTTVLLDRASFTIDKGERVCLIGRNGMGKST
                     LLKIVNGDVKVDDGEIVRAQNLKVALLAQEVPPATDGTVYDVVAQGLGAAGAAVAHYH
                     HLLQDPEVDLDKLGKAQAVLEAHDGWTLETRVQETVTRLQLPPDAPFASLSGGMKRRV
                     MLGRALVAGPDLLMLDEPTNHLDIESIEWLEQFLKEFEGAILFVTHDRAFLRNLATRI
                     AELDRGWLTSWPGDYDNYLRRREERLHAETVERALFDKRLAAEEKWIRKGIEARRTRD
                     QGRVKRLMEMRAQFSQRRHAPGQARMQVQEAERSGKLIAEAIDASFSYGGRTIVHKLN
                     CTLLRGDKLGIIGPNGAGKTTLLKLLLGELEPTSGSIKLGSKQQVAYFDQLRAVLDPN
                     KSVLDNVAEGKDYVEAGGVRKHVIGYLQDFLFAPDRARAPVQTLSGGERNRLLLAKLF
                     AKPSNLLVLDEPTNDLDVETLELLEELLLDYTGTVLLVSHDRAFIDAVVTDSLVFEGN
                     GRVNEYVGGYSDWQRQRPEPVAAAAPVKAKLAPAAAPVPALDAKEQKELGALPGKIEK
                     LEARQQEINARFAEPDFYTRDAATVTTLQKELAALEQELQSVYARWEALEARRNA"
     gene            complement(5090..5161)
                     /locus_tag="VHE37_12145"
     tRNA            complement(5090..5161)
                     /locus_tag="VHE37_12145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5127..5129),aa:Thr,seq:tgt)
     gene            complement(5193..>5531)
                     /locus_tag="VHE37_12150"
     CDS             complement(5193..>5531)
                     /locus_tag="VHE37_12150"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HVT36334.1"
                     /translation="VSEQGRHLGGIIAPGLLTAQRAVLGATRFATRDERAHYTLELGR
                     DTEACVRQGAYHACVGAIVQASAPAGARAQKFITGGDAALLARGLDAGWQQGPQLVLE
                     GLLALALDST"
BASE COUNT          910 a         1932 c         1802 g          887 t
ORIGIN      
        1 ccgatacctt cagcctgcgc ggcgtgtaca agtccaccga cggcgcgacc tggacttcag
       61 cgagcagcgg tttgccggcc aatcccagca tccgcaacct ggcgatccgc ggcaccaagc
      121 tgtgggtgta cctcggcggg cagggcctgt tccgcagcac cgatggcgcc gccagctggc
      181 agaacgtcac caccggcctg tcgggcgacc tgttcggaaa agtgttcgcg gcatccaccg
      241 tgctgatcgc cagcgccggc ggcgtggtct accgctccac cgacgatggc gacacctggg
      301 ctgcgaccac cggctacgcg gtgagcaagt tcggcatcga gatcctgtcg atggccgagg
      361 acgccgccgg cgcgctgtac ctgggcgacg agcatgcggg cctgtcggtg tcgaaggacg
      421 ccggcgtcac ctgggcaccg tcgagcaacg gcctggccac cagcaacgtg tgcatcgagc
      481 gcatcaactc gctgctgttc aagggcggca agatctacgc cggccagggt gaaggcggcg
      541 gcgtgcgcgt gtccagcccg atcggcgcgg cggcgccggg cgacagcgtg gcctgcacca
      601 ccgacaagga catcttcccc aataacttct ccttcaccgc gcagaccaac gtcaagccca
      661 gcacgctggt gaagagcaac atcatcaccg tcgccggcct gggcaacggc gtcagcgtgc
      721 cgatctcggt gagcggcggc gagttcgcca agtccgccac ctgcggcgcc ggtttcacca
      781 ccaccaccgg ccacgtggtc aatggcgacc aggtgtgcgt gcgccacacg tccgcggcca
      841 ccagcgaaac ttcggtgaag acgcggctga ccatcggcga caagagcgcc actttcacct
      901 cgagcacggg cctggtattc accggccgca ccgacgagat caacaccggc ttcggcagca
      961 atggatttgc cagcgtggcc agccagacca cggcatcgga cgtgaagatc ctggccgacg
     1021 gcagcttcgt cagcgccgcc gccaccagcg gcttcccggc cgacatcgcc atcaccaagt
     1081 tcaactacga cggcagcgtc gacaccagct tcggcgtcgg cggcgtggcc aagttcgacg
     1141 ccggcggcag cgagagcgtg gtggcggtga tcgcgcagtc cagcggcaag ctggtggcgg
     1201 tggccaacac gcgcatcggc acgccgagcg cgaccaacaa gatcgtgctg gtgcgcttca
     1261 acgccgacgg cagcatcgac agcggcttcg gcaccagcgg catggtcact ttcgacctca
     1321 accccaacca gtcgggcaac ggcgagaacg ccagcagcgc cattgcgctg tccgatggct
     1381 cgatactcat cgggggcggc gtcgccatca ccggcaccgg cacgcacttc ttcgtggtca
     1441 agttctcggc cgatggcgtg caggacacct ccttcggcac ggccaacgcc acgctcggtt
     1501 cgaccttcgc cgacgtgcag gtgggcagca gcacggtggt cggcggcgcg ctcgcggtgc
     1561 aaagcgatgg caaggtgctg atgtccgggc aggcggatgt gggcttcaac accatccgcg
     1621 gactggtggt gcgcttcacc agcgccggcg tgctcgacag cggcttcggc agcagcggca
     1681 agttcgcttc cgccagcgcc attgccttgc ccgcgctgct ggtgctgggc gacggcagca
     1741 tcatggcggc gggccagacc accagttcgc cgttctcgcc ggtgatgacg cgcctgacca
     1801 gctccggcgc gctcgacacc agcttcggca gcagcggcgt gctcacgctg acgctctccg
     1861 gcaattcatc gtcgggcagc ctctcctcgc tgctgctgct cgccaacggc ggcattgcct
     1921 tcagcggcca ggcttaccag agcgacctgg gcgccagcgc gctgctgttc ggcatcctca
     1981 acagcaatgg cacgctggac accagcttcg gcaacaacca cggctacctg gtgcgccggc
     2041 cggtgaacgg cagcaacgtg ctgggcgggc gcatggcgca gcgcgccgac ggcggcgtgc
     2101 tgttcggcgg cctctacggc agctcgctcg gcgccttcgt cttcggcggc acttccggcc
     2161 cgccgccgga caccacgccc aacgcgttca ccttcgccac caagaccgcg gtgtcgcgca
     2221 acgtgcaggt gacgtccgcg gccaaggcga tcaagggcat caacgcgccg gcgccggtaa
     2281 ccatcagtgg cggcacctac tcgatcggca ccaccgattg cggcaacacc tacaccagcg
     2341 ccgcgggcat gatcagcaac gggcagaaga tctgcctgcg ccacacctcg tcgcgcagcc
     2401 cggcgaccaa gaccagcacc atcgtgacga ttggcggggt cagcggcacc ttcagttcgg
     2461 tgacggtcga cgacgtgccg gccgccttca agttcaccga cgcggcgggc gttacgccgt
     2521 cgagcgtggt ggtgtccgac accgtcaccg tgtcgggcat cgatgcgccg gccgccgtgt
     2581 cagtgaagaa cggcgagtac tcgatcggca tcagcgattg cggcaacacc ttcaccagcg
     2641 ccgacggcac catcagcaac ggccagatga tctgcgtgcg ccacaccgcg gccgccggca
     2701 acaacaaggc gatcaacacg gtgctgatca tcggcggcgt gcaggacacc ttcagctcgg
     2761 tgacagccaa gggcaccggc accgcggacg tgatgcccaa cttcttccac ttccccgacg
     2821 tgaacaacgc ggcgccgtcg acgctgaccg cttcgcaggc gctgaccatc gcgggcatcg
     2881 ccaacggctc gaccgcggtg gtgaaagtga cgggcggcga gtactcgatc gacggcggcg
     2941 cgttcacctc ggccgacggc accatcggct ccggccagac catcgcggtg cgcctgacca
     3001 gttcggccaa cgcctccacg ccggtcagcg ccacgctcaa catcagcggc ctgaccgacg
     3061 cgttcaccgt caccaccggc aagtagcgcg caaaaacgag gtcaccgcac cccggatttc
     3121 gatgttatat tttattgtct gtttattaaa ttatacaata attaataaca tcgattccgg
     3181 cggtgcggtg gaggggtttc aggcgttgcg gcgtgcttcc agcgcttccc agcgcgcgta
     3241 gacggactgc agttcctgct cgagcgcggc gagttccttc tgcagcgtcg tcaccgtcgc
     3301 ggcgtcgcgc gtgtagaaat ccggctcggc aaaacgcgcg ttgatttcct gctggcgcgc
     3361 ttcgagcttt tcgatttttc ccggcagcgc gccgagttcc ttctgctcct tcgcgtccag
     3421 cgcgggcacc ggtgcggccg cgggcgccag cttcgccttc accggcgcgg cggcggcgac
     3481 cggctccggg cgctgccgct gccagtcgga atatccgccg acgtattcat tgacgcggcc
     3541 gttgccctcg aacaccaggc tgtcggtgac cacggcgtcg atgaatgcgc ggtcgtgcga
     3601 caccagcagc acggtgccgg tgtaatcgag cagcagctct tccagcagct cgagcgtttc
     3661 cacgtcgaga tcgttggtcg gctcgtcgag caccagcagg ttcgacggct tggcgaagag
     3721 cttcgccagc agcaggcggt tgcgctcgcc acccgacagc gtctgcaccg gcgcgcgcgc
     3781 gcgatccggc gcgaacagga agtcctgcag atatccgatc acgtgcttgc gcacgccgcc
     3841 ggcctccacg tagtccttgc cctcggccac gttgtcgagc accgacttgt tcggatcgag
     3901 caccgcgcgc agctggtcga agtacgccac ctgctgcttg ctgccgagct tgatgctgcc
     3961 ggaggtgggt tccagttcgc cgagcagaag tttcagcagc gtggtcttgc cggcgccgtt
     4021 ggggccgatg atgccgagct tgtcgccgcg cagcagcgtg cagttgagtt tgtgcacgat
     4081 ggtgcggccg ccgtaggaaa agctggcgtc gatggcctcg gcgatcagct tgccgctgcg
     4141 ctcggcttcc tgcacctgca tgcgcgcctg ccccggcgca tggcggcgct gcgaaaactg
     4201 cgcgcgcatt tccatcagcc gcttgacgcg gccctggtcg cgcgtgcgcc gcgcctcgat
     4261 gcccttgcgg atccatttct cctcggcggc caggcgcttg tcgaacaacg cgcgctccac
     4321 cgtttccgcg tgcaggcgct cctcgcgccg gcgcaaataa ttgtcgtagt cgcccggcca
     4381 cgaagtgagc cagccgcgat ccagctcggc aatgcgcgtg gccaggttgc gcaggaacgc
     4441 gcggtcatgc gtgacgaaga gaatcgcgcc ttcgaattcc ttgaggaact gctccagcca
     4501 ctcgatgctc tcgatatcga gatggttggt cggctcgtcg agcatcagca ggtccggccc
     4561 ggcgaccagc gcgcggccga gcatcacgcg ccgcttcatg ccgcccgaca acgaggcgaa
     4621 cggcgcgtcc ggcggcagct gcagccgcgt caccgtttcc tgcacgcgcg tctccagggt
     4681 ccagccgtcg tgcgcttcca gcaccgcctg cgccttgccc agcttgtcga gatcgacctc
     4741 ggggtcctgc agcaggtggt ggtagtgcgc caccgccgcg ccggccgcac cgagaccttg
     4801 cgcgaccacg tcgtacaccg tgccgtcggt ggccggcggc acttcctgcg ccagcagcgc
     4861 caccttcagg ttctgcgcac gcacgatctc gccgtcgtcg actttcacgt cgccgttgac
     4921 gatcttgagc agcgtggact tgcccatgcc gttgcgcccg atcaggcaca cgcgctcgcc
     4981 cttgtcgatg gtgaagctgg cgcggtcgag cagcacggtg gtgccgatct tgagggacag
     5041 atcgcgcagg gtcagcagaa gcatggggaa gaaaacctgc gtaagggtat gccgggtgag
     5101 ggaatcgaac ccccgaccaa cggtttacaa aaccgctgca ctaccgctgt gctaacccgg
     5161 cgtcggtgtg tgagaaaaaa gcggcgcgca gtctaagtcg agtccagcgc cagcgccagc
     5221 aagccttcca gcaccagctg cgggccctgc tgccagccgg cgtcgaggcc gcgcgcgagc
     5281 agcgcggcgt cgccgccggt gatgaatttc tgcgcacgcg cacctgccgg cgcgctggcc
     5341 tgcacgatgg cgccgacgca ggcgtggtac gcgccctggc gcacgcaggc ctcggtgtcg
     5401 cggcccagtt ccagcgtgta gtgcgcgcgc tcgtcgcgcg tggcgaagcg cgtggcgcca
     5461 agcacggcgc gctgcgcggt gagcaggccc ggcgcaatga tgccgccgag atggcggccc
     5521 tgctcgctga c
//
LOCUS       DASZZC010000570         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_559774,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000570 DASZZC010000000
VERSION     DASZZC010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559774"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1440
                     /locus_tag="VHE37_12155"
     CDS             349..1440
                     /locus_tag="VHE37_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HVT36335.1"
                     /translation="MPILQSLFGGGEEPLIGLDISSSSVKLLELSRRGDRFHVETYAV
                     EPLPPNAVNDKQIADPKVVGEVVGRAVSRAGTRTKNAAVAVAGASVISKVIQMPASLK
                     EDEMEEQIKAEADQYIPYPIDEVNLDFQVIGAAGKGGEMSDVMLAACRKEQVEQRCAA
                     VELAGLVPKVVDIESYALENACQFLRHQMPDEGNGKTVAIVDMGASTTSVLILNDMKT
                     IYTRDQAFGGKQLTEDIMRNFGMSFEEAIRAKKSGNLPDNYQTDVLAHFIADMAQQVD
                     RSLQFFFSAGSQYTNVDQIILAGGCAHIANVDTAIQDKLKIPTVVAKPFAQMSVASKA
                     KPAALQKEEASLMIAAGLAWRAFDEAPKS"
     gene            1440..2012
                     /locus_tag="VHE37_12160"
     CDS             1440..2012
                     /locus_tag="VHE37_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HVT36336.1"
                     /translation="MRINLLDWRQARRDLRKKQFLTLLGLGAGAALALVLFGYFIASQ
                     AVQHQNARNDYLRAQIAETDQKIAEIQDLEKTRANLLARMRVIEELQASRSATVHFFD
                     EIVNTLPDGVSITSLRQAGANVTITGVAESTGRISTYLKNLDASTWFADPKLIEIKTF
                     EKDKQRKAEFTLQVRNLTKAGNKKTQENAQ"
     gene            2009..2698
                     /gene="pilO"
                     /locus_tag="VHE37_12165"
     CDS             2009..2698
                     /gene="pilO"
                     /locus_tag="VHE37_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HVT36337.1"
                     /translation="MTLEERFQELQSLDPNNPGAWPLWVRIGAVIVAALVILGGGYKL
                     AVQPQLDELSRQQAQENSLFDDFQSRQKKVAALDAYREQLKEMEKLFGNMLRLLPSKA
                     EVANLLNDISQTRVAASLEEELFRPMADVPKDFYAELPNQIVVNGNYHQMGQFVSGVA
                     ALPRIVTIEDVDIRPMGQPANNGGKPAVPTEGSPVLHMQAIAKTYRYLDESEQAASKP
                     SGPPGTPGAPR"
     gene            2695..3213
                     /locus_tag="VHE37_12170"
     CDS             2695..3213
                     /locus_tag="VHE37_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HVT36338.1"
                     /translation="MNRATRRLLSLALVLLLAACSAGMADLEQKVNDVKKQKSTAIEP
                     IPQIKQYEAYAYDAAGRRDPFAAVEPKRNASGFDSNGLKPDLNRNKEPLEEFTLDSLR
                     MVGIIESGGKTWAMVKAPDKVIHRVTLKDHMGQNYGEIVKITESEVSLMEIVPDGFGG
                     WMQRPASLALAQ"
     gene            3249..>4499
                     /locus_tag="VHE37_12175"
     CDS             3249..>4499
                     /locus_tag="VHE37_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HVT36339.1"
                     /translation="MYKSNKITRNLAAALAMLWLLAAGAQESGGRTLQALDVVGLEGE
                     RVLMTLTLSEPAPAPVVFTIDKPARLSLDLPDTHLGLVDRFRKIGIGKALSVAAVEAK
                     GRTRVVVELADPAPYNVKVEGNKILVQLDSGAVATAANAAVSVAPQLAGALLPRGPAI
                     TNVDFRRGEKGEGRVVVTLADPKTGVDVNEVGGKVVARFKNAPLSDKQVKRLDVLDFA
                     TPVKYVDVQRAGSDTEITVTPAEGADFEQVAYQAGNVFTLELQPLTAEKVEERKKQHP
                     QFTGERVSLSFQSVDIRSLLQIIADVAGTNMVVSDQVNGEIAMRLQNVPWDQALDIIL
                     RTKGLGMRQQGNVMLVAPLEELATRDKVELEAQKTNTELAPLRSELIQVNYAKAGELS
                     KLLKGCGTATCATNAKNSILSDRGH"
BASE COUNT          918 a         1423 c         1469 g          689 t
ORIGIN      
        1 tcgcgcgtgt agatgcgcag cggtacctgc agctgcaatg cacgcaagga ttccaccgac
       61 ggcagctgcg gttcgatgta cgcgcgcagg ccgatatagc ccagcaccgc ggcgagcagc
      121 gcgagggcaa aaagcgccgc cagcgatagg agaagtttgc gtcgcatgga gtaggagttt
      181 gacgcgcttt tagggggcag aaatggtgct caagtaatgg agcactatca gatagttgca
      241 atacaaactt cgagttgcat tgtagaatta ttgcgttata ttaaggcgca atgcgggagt
      301 agtcactgat tagcccgctc tctcatattg gggtgcgcga ggggcgatat gcctattctt
      361 caatcgctgt ttggcggagg cgaggagccg ctgatcgggc tggatatcag ctcgagctcg
      421 gtcaagctcc tggaactttc gcgccggggc gatcgcttcc acgtcgagac ctacgcggtg
      481 gagccgctgc cgcccaacgc cgtcaacgac aagcagatcg ccgatcccaa ggtcgtcggt
      541 gaagtggtcg gccgcgcggt cagccgcgcc ggcacgcgca ccaagaacgc ggcggtcgcg
      601 gtggccggcg cctcggtcat ctccaaggtc atccagatgc cggcttccct caaggaagac
      661 gagatggagg agcagatcaa ggccgaggcc gaccagtaca tcccgtaccc gatcgacgaa
      721 gtgaacctgg acttccaggt gatcggcgcc gcgggcaagg gcggcgagat gtcggacgtg
      781 atgctggcgg cgtgccgcaa ggagcaggtg gagcagcgct gcgcggcggt cgaactggcc
      841 gggctggtgc ccaaggtggt cgacatcgaa tcctacgcgc tggagaacgc ctgccagttc
      901 ctgcgccacc agatgccgga cgagggtaat ggcaagacgg tggccatcgt cgacatgggt
      961 gccagcacca cctcggtgct gatcctgaac gacatgaaga ccatctacac gcgtgaccag
     1021 gccttcggcg gcaagcagct gaccgaggac atcatgcgca acttcggcat gagcttcgag
     1081 gaagccatcc gcgccaagaa gagcggcaac ctgccggaca actaccagac cgacgtgctg
     1141 gcgcacttca tcgccgacat ggcgcagcag gtggaccgct cgctgcagtt cttcttctcc
     1201 gccggctcgc agtacaccaa cgtcgaccag atcatcctcg ccggcgggtg cgcgcatatc
     1261 gccaacgtcg acaccgccat ccaggacaag ctgaagatcc ccaccgtggt ggccaagccg
     1321 ttcgcgcaga tgagcgtggc gtccaaggcc aagccggcgg cgctgcagaa ggaagaggca
     1381 tcgttgatga tcgcggccgg cctggcctgg cgcgcgttcg atgaggcgcc caagtcatga
     1441 tgcgcatcaa cctcctcgac tggcgccagg cacggcgcga cctgcgcaag aagcagttcc
     1501 tgacgctgct cggcctgggc gccggggccg ccctcgcgct ggtgctattc ggttacttca
     1561 tcgccagcca ggcggtgcag caccagaacg cgcgcaacga ctacctgcgc gcgcagattg
     1621 ccgagaccga ccagaaaatc gccgagatcc aggacctgga aaagacccgc gccaacctgc
     1681 tcgcgcgcat gcgcgtgatc gaggaactgc aggccagccg ttcagcgacc gtgcatttct
     1741 tcgacgagat cgtcaacacg ctgcctgacg gcgtctccat cacttcgctc aggcaggccg
     1801 gcgccaacgt caccatcaca ggtgtggccg agtccaccgg gcgcatctcg acctacctga
     1861 agaacctcga tgcctcgacc tggttcgccg atcccaagct gatcgagatc aagaccttcg
     1921 agaaggacaa gcagcgcaag gccgagttca ccctgcaggt gcgcaacctg accaaggccg
     1981 gcaacaagaa gacgcaggag aacgcgcaat gaccttggaa gaacgtttcc aggaattgca
     2041 gagcctggac cccaacaacc ccggcgcctg gccgctgtgg gtgcgcatcg gcgcggtgat
     2101 cgttgcggcc ctggtgatcc tgggcggcgg gtacaagctc gccgtgcagc cgcagctcga
     2161 cgaactgtcg cgccagcagg cgcaggaaaa ttccctgttc gatgacttcc agtcgcgcca
     2221 gaagaaagtg gcggcgcttg acgcctaccg cgaacagctc aaggaaatgg agaaactttt
     2281 cggcaacatg ctgcgcctgc tgccgagcaa ggccgaggtg gcgaacctgc tcaacgacat
     2341 ctcgcagacg cgcgtcgccg ccagcctgga agaggaactg ttccggccga tggccgacgt
     2401 gcccaaggat ttctacgccg agctgcccaa ccagatcgtg gtcaacggca actatcacca
     2461 gatgggccag ttcgtcagcg gcgttgccgc gctgccgcgc atcgtgacca ttgaggacgt
     2521 ggacatccgg cccatggggc aaccggccaa caacggtggc aagccggcag tgcccaccga
     2581 gggttcgccg gtgctgcaca tgcaggccat cgccaagact tatcgctatc tcgatgagag
     2641 tgagcaggcg gccagcaaac cttcaggtcc tccgggtact ccgggagcgc cgcgatgaac
     2701 cgcgcgacgc gcaggctgct gtcgctggca ctggtgctgc tgctggccgc gtgcagcgcc
     2761 ggcatggccg acctcgagca gaaggttaac gacgtcaaga agcagaaatc gaccgccatc
     2821 gaaccgattc cgcagatcaa gcagtacgag gcctacgcct acgatgctgc cggccggcgc
     2881 gacccgttcg ccgccgtcga acccaagcgc aatgcctcgg gcttcgacag caatggcctc
     2941 aagcccgatc tcaaccgcaa caaggaaccg ctcgaggagt tcaccctcga ttcgttgcgc
     3001 atggtcggca tcatcgagtc gggcggcaag acctgggcca tggtcaaggc gccggacaag
     3061 gtgatccatc gcgtcacgct caaggaccac atggggcaga actacggcga gatcgtgaag
     3121 atcaccgagt ccgaagtcag cctgatggaa atcgtgccgg acggctttgg cggctggatg
     3181 cagcggccgg cctcgctggc gttggcgcaa tagcggccgc gcagggaaaa attctgggag
     3241 ttgcagctat gtacaagtcc aacaaaatca cccgcaacct cgctgcagcg ctggccatgc
     3301 tctggctgct cgcggcgggc gcgcaggaaa gcggcggccg cacgctgcag gccctcgacg
     3361 tggtcggcct ggaaggagaa cgcgtgctga tgacgctgac cctgtcggag ccggctccgg
     3421 ctccggtggt gttcaccatc gacaagccag cgcggctatc gctcgatctg ccagatactc
     3481 acctgggcct ggtggaccgc ttccgcaaga tcggcatcgg caaggccttg tcggtggccg
     3541 cggtcgaggc caagggccgc acgcgcgtgg tggtcgaact ggccgatcct gcgccgtaca
     3601 acgtcaaggt ggaaggcaac aagatcctgg tgcagctcga cagcggcgcg gtagcgacgg
     3661 ccgccaacgc cgcggtatcc gtggcgccgc agctggcggg cgcgctgctg ccgcgcgggc
     3721 cggccatcac caacgtggat ttccgccgcg gcgagaaggg tgagggccgc gtagtggtga
     3781 ctctggccga ccccaagacc ggcgtcgacg tcaacgaggt gggcggcaag gtggtggcgc
     3841 ggttcaagaa cgcgccgctg tccgacaagc aggtcaagcg gctcgacgtg ctggacttcg
     3901 ccacgccggt caagtacgtc gacgtgcagc gcgccggcag cgataccgag atcaccgtga
     3961 cgccggccga aggcgcggac ttcgagcagg tggcctacca ggccggcaac gtgttcactt
     4021 tggagctgca gccgctgacg gcggagaagg tggaagagcg caagaagcag catccgcagt
     4081 tcaccggcga gcgcgtatcg ctgtcgttcc agagcgtcga catccgctcg ctgctgcaga
     4141 tcatcgccga cgtggccggc accaacatgg tggtgagcga ccaggtcaac ggcgagatcg
     4201 ccatgcgcct gcagaacgtg ccgtgggacc aggcactgga catcatcctg cgcaccaagg
     4261 ggctgggcat gcgccagcag ggcaacgtca tgctggtggc gccgctggaa gaactggcca
     4321 cgcgcgacaa ggtcgagctg gaagcgcaga agaccaacac cgaactggcg ccgctgcgct
     4381 ccgagctgat ccaggtcaac tacgccaagg ccggcgagct gtccaagctg ctcaagggtt
     4441 gcggcaccgc gacctgcgcc accaatgcca agaattccat cctgtccgat cgcggacat
//
LOCUS       DASZZC010000571         6120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_952725,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000571 DASZZC010000000
VERSION     DASZZC010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952725"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VHE37_12180"
     CDS             complement(<1..323)
                     /locus_tag="VHE37_12180"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HVT36340.1"
                     /translation="MKFDDKHSFDKPAATVIKMFSDQKYFERKYKDLGGWDINVLECE
                     KSDKKFRIKVRYTMKSNVPLPEFAKKFVPDTAVITQTDSWDIASRTGRLEADIKGAPV
                     KVAAD"
     gene            complement(397..1749)
                     /locus_tag="VHE37_12185"
     CDS             complement(397..1749)
                     /locus_tag="VHE37_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT36341.1"
                     /translation="MMKKIASIAAIVALGASLQVVAKVSPEEAAKLGKELTPIGAEKG
                     PNKDGTIPEWTPLTVGGSLKGEYASDPTVDNEKPLFTITKANMAQHADKLMEGQKKLL
                     STYDSYKLNVYPSHRKANFPEEIKKATIENATRADMQGTDDPIGAAEGFPFPIPKSGA
                     EPIWNHKLKWRGEAVTRYNNQMIVQPNGDYQLTKIIEDVEFSYASIKHPVPLDATHKE
                     FLKYLSHTVAPPRLAGTYILVIEKASTGNEGRAAWLYSPGLKRIRRAPTVCCDNPYEG
                     TDGHQFYDQVDMFNGALDRYNWKLVGKKEMYIPYEGNRLGGPQTKFKDMAKPHHLNAD
                     LPRYELHRVWIVEATLKPGTSHTFKRRVFAIDEDSWDISMVDDYDNRDQLFQFQEGHL
                     ALGKNIGSVSSVPEVIYHFDSGRYFITAMSNEDKPNDFTVHFTDDYFTAASVQKNTTK
                     "
     gene            complement(1922..3277)
                     /locus_tag="VHE37_12190"
     CDS             complement(1922..3277)
                     /locus_tag="VHE37_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT36342.1"
                     /translation="MLKRTMLLAAALLGLTALPALAKVDAAEAAKLGKDLTPVGAEKA
                     ANKDGSIPAWTGGTPKHGALSNEYPNDPKIDGDKPLFTITKANMDQYKDMLTEGHKYL
                     LKTYDDYKMNVYPSHRTVAWPDEIYKATAANATTATLEGTDVLLDASLGFPFPIPKTG
                     AEVIWNHKVKYRGEAVRRYNNQMIVQPNGDFQLTKIVEEVKFYYASIKNPLKLTKDNQ
                     LFLKYLSQTIAPPRMAGTFILVHEKGGTGDNGRAAWLYSPGLKRIRRAPTVCCDNPYE
                     GTDGHQFYDQVDMFNGVLERYDWKLVGKKEMYLPYNSDKIAGPSVKYKDLAKGHHLNQ
                     DLPRYEKHRYWVVEANIKPGTNHTFHKRVFYVDEDAWTIEAVDDYDAKNLLFQFQEGH
                     LITGSNILSASTVPEVIYHFDSGRYFITAAFNEDKPYDLTVNFDDSYFEAASVQKHTT
                     K"
     gene            complement(3485..4690)
                     /locus_tag="VHE37_12195"
     CDS             complement(3485..4690)
                     /locus_tag="VHE37_12195"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HVT36343.1"
                     /translation="MSAGLDPDLNAAVVDLSAELIPMGSTSPSSAARKASSAEVDELL
                     ESLSRTMRAEEMAKAAAKAEAAANAPPPSPFDFEWHPVKGCRLRASAACNDCPSATSE
                     MPVASETQLMDPIRLRKAMDILVAPQSDLFQAEIDDNLRTRIFTVMKNAGHHRFFVLT
                     KNPGYMRRYFQRAQLPTLPNVWLGVSVEDQASFDTRVYELMRTPAANRWVAFEPLLGR
                     INTKTVQLPTFDLYHPWRGIVQAYEYMDEKGQRHWAPDDAAPLQELPIPRWIYVAGQK
                     GAKAVPPHPSWVKQILGEAEGISAWFGGFGEYTQTEKADLERDATLLYMNSTGELRGR
                     GAGSKTNFLAREFDGETSAIFTRTGTPRSRLLLDGASLLQRAPNAVALRSGIRATPFN
                     LTGINPPRA"
     gene            5026..5619
                     /locus_tag="VHE37_12200"
     CDS             5026..5619
                     /locus_tag="VHE37_12200"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVT36344.1"
                     /translation="MAVVAVLVAGGAAAAASDDNPIYLYRQSNGVRVYTDRKPDHSAW
                     VIIAKYGRPTASASCTGLTAQSLDARASTYAPLIAKYASARGVPAALVRAVMRVESCF
                     DHRAVSRVGARGLMQLMPETAAEMGVSDSFDPEQNISGGVHYLSMMLERFHQNRELAL
                     AAYNAGPEAVEKHGGIPPFPETQSYVRRVIASYRQQT"
     gene            5689..>6120
                     /locus_tag="VHE37_12205"
     CDS             5689..>6120
                     /locus_tag="VHE37_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT36345.1"
                     /translation="MKYTRIGMAAALCGIVVSAQAAAPAWVAESNRNTDVLLQQQARF
                     NPENASAQGVEGYDDQVVDLQPKVYERSQAAAADAVKELQRRLAAASDPQVKQDLEIL
                     IGSAQDGIETARLNNDELLPYFSVARIVFSGMQSLLDPRVSK"
BASE COUNT         1006 a         1875 c         1992 g         1247 t
ORIGIN      
        1 atgtccgcgg ccaccttcac cggcgcacct ttgatgtccg cttccaggcg gccggtgcgg
       61 ctggcaatgt cccagctgtc ggtctgcgtg atcaccgcgg tgtcgggcac gaacttcttg
      121 gcgaactccg gcaatggcac atttgacttc atggtgtagc gcaccttgat gcggaacttc
      181 ttgtcgctct tctcgcactc cagcacgttg atgtcccatc cgcccaggtc cttgtacttg
      241 cgttcgaagt acttctgatc ggaaaacatc ttgatcacgg tggcagccgg cttgtcgaaa
      301 ctgtgcttgt cgtcgaattt catgatctct cccctgaatg aaaacgccgc cggctgagcg
      361 aacaaccggc ggcggataag cagcgttgcc tgaagcttac ttggtcgtgt tcttctgcac
      421 cgatgcggcg gtgaagtagt cgtcggtgaa atgcacggtg aaatcgttgg gcttgtcttc
      481 gttgctcatg gcggtgatga agtagcggcc cgaatcgaag tggtagatca cttccggtac
      541 cgacgacacc gagccgatgt tcttgcccag ggccaggtgg ccttcctgga actggaacag
      601 ctggtcgcgg ttgtcgtagt cgtcgaccat cgagatgtcc cagctgtcct cgtcgatggc
      661 gaacacgcgg cgcttgaaag tgtggctggt gccgggcttc agcgtcgctt cgacgatcca
      721 cacgcggtgc agttcgtaac gcggcaggtc ggcgttcagg tggtgcggct tggccatgtc
      781 cttgaacttg gtctgcggcc cgcccaggcg gttgccttcg tagggaatgt acatctcctt
      841 cttgccgacc agcttccagt tgtagcggtc gagcgctccg ttgaacatgt ccacctggtc
      901 gtagaactgg tgaccgtcag tgccttcata aggattgtcg cagcacaccg tcggtgcgcg
      961 gcggatgcgc ttgaggccgg gcgagtacag ccaggcggcg cggccctcgt tgccggtcga
     1021 ggccttctcg atcaccagga tgtaggtgcc ggccagccgc ggcggcgcca ccgtgtgcga
     1081 caggtacttg aggaattcct tgtgcgtggc gtccagcggc accgggtgct tgatgctggc
     1141 gtaggagaac tccacgtcct cgatgatctt ggtcagctgg taatcgccat tgggctgcac
     1201 gatcatctgg ttgttgtagc gggtgaccgc ctctccgcgc cacttcagct tgtggttcca
     1261 gatcggctcc gcgccgctct tgggaatcgg gaacgggaag ccttccgcgg cgccgatcgg
     1321 atcgtcggtg ccctgcatgt cggcgcgcgt ggcgttctcg atggtggcct tcttgatctc
     1381 ttcggggaag ttggccttgc ggtggctggg atagacattg agcttgtagc tatcgtaggt
     1441 cgacagcagc tttttctggc cttccatcag cttgtcggca tgctgcgcca tgttcgcctt
     1501 ggtgatggtg aacagcggct tttcgttgtc gacggtcgga tcggacgcgt actcgccctt
     1561 gagcgaaccg ccaacggtca gcggcgtcca ctcgggaatg gtgccatcct tgttcgggcc
     1621 tttttcagcg ccgatcgggg tcagttcctt gcccagcttg gccgcttctt cggggctcac
     1681 cttcgccacc acctgcagcg aggcgccgag ggcgacgatc gcagcgatcg acgctatttt
     1741 tttcatcatg gatattgctc ctcttgctat gttgggcgtg cagcagcggc ccttctgggt
     1801 ggcggcgatg cgaacgaaaa atttgcgcat ctgacaaaca gacaacgcgc gcacaaaaaa
     1861 agccgccgga ttgctccggc ggccatgaaa aaaatgatgc ccgattcggt tcggtgaacg
     1921 cttatttggt ggtgtgcttc tgcaccgagg ccgcttcgaa ataggaatcg tcgaagttca
     1981 ccgtcaggtc gtacggcttg tcctcgttga acgccgccgt gatgaagtag cggcccgagt
     2041 cgaagtggta gatcacttcc ggcacggtgc tggcggacag gatgttggag ccggtgatca
     2101 ggtggccttc ctggaactgg aacagcaggt tcttggcgtc atagtcgtcc accgcctcga
     2161 tggtccatgc atcttcgtcg acgtagaaca cacgcttgtg gaaggtgtga ttggtgcccg
     2221 gcttgatgtt ggcctcgacc acccagtagc ggtgcttctc gtagcgcggc agatcctggt
     2281 tcaggtgatg ccccttggcc aggtccttgt acttcacgct ggggccggcg atcttgtcgg
     2341 agttatacgg caggtacatc tccttcttgc cgaccagctt ccagtcgtag cgctcgagca
     2401 cgccgttgaa catgtccacc tggtcgtaga actggtggcc gtcggtgcct tcatacggat
     2461 tgtcgcagca caccgtcggc gcgcgccgga tgcgcttcag gcccggcgag tacagccagg
     2521 cggcgcggcc gttgtcgccg gtgccgccct tctcgtgcac caggatgaaa gtgccggcca
     2581 tgcgcggcgg cgcgatggtc tgcgacaggt acttgaggaa cagctggtta tccttggtca
     2641 gcttcagcgg attcttgatc gaggcgtaat agaacttcac ttcctcgacg atcttggtca
     2701 gctggaagtc gccgttgggc tgcacgatca tctggttgtt gtagcgccgc accgcttcgc
     2761 cgcggtactt caccttgtgg ttccagatca cctcggcgcc agtcttggga atcgggaacg
     2821 ggaagccaag cgaggcatcg agcaggacat cggtcccttc cagcgtggcg gtggtggcgt
     2881 tggccgcggt ggccttgtag atctcgtcag gccaggccac cgtgcggtgg ctcgggtaga
     2941 cgttcatctt gtaatcgtcg taggttttga gcaggtactt gtgaccctcg gtgagcatgt
     3001 ccttgtactg gtccatgttc gccttggtga tggtgaacag cggcttgtcg ccgtcgatct
     3061 tcggatcgtt ggggtactcg ttcgacagcg cgccgtgctt gggcgtgccg ccggtccagg
     3121 cgggaatgct gccgtccttg ttggccgctt tctcggcgcc aaccggcgtc aggtccttgc
     3181 ccagcttggc ggcttccgcc gcatccacct tggccagcgc gggcagcgca gtaagcccca
     3241 gcagcgcggc agcgagcagc attgttcttt taagcatcgc gttctcctcg ttgagcagga
     3301 gcgcgatgct atgcgagtag ctatggattt gccaccgccg cacacaacgc aaaccttcag
     3361 gaaaaactgg cgtggatgcc gaccgcaccg ccctgattga tgttattaat gattgactta
     3421 attttaatta agtcaatatt tagtaaccgc ccgatgccgg gtgcggtgga gtggctggag
     3481 cagcctaggc gcgcggcgga ttgatgccgg tgagattgaa tggcgtggcg cggatgccgc
     3541 tgcgcagcgc cacggcgttg ggcgcgcgct gcagcagcga agcgccatcg agcagcagcc
     3601 gcgagcgcgg cgtgccggtg cgcgtgaaga tggcggacgt ctcgccgtcg aattcgcgcg
     3661 ccaggaaatt ggtcttgctg ccggcgccgc ggccgcgcag ctcgccggtg gaattcatgt
     3721 agagcagcgt ggcgtcgcgt tcgaggtcgg ccttctcggt ctgcgtgtat tcgccgaagc
     3781 cgccgaacca ggcgctgatg ccttcggcct cgccgaggat ctgcttgacc caggacgggt
     3841 gcggcggcac cgcctttgca cctttctggc cagcaacgta aatccagcgc gggatcggca
     3901 gctcctgcag cggcgccgca tcgtccggcg cccagtggcg ctggcccttc tcgtccatgt
     3961 attcgtaggc ctgcacgatg ccgcgccacg ggtgatacag gtcgaaagtg ggcagctgca
     4021 ctgtcttggt gttgatgcgg cccagcagag gctcgaacgc cacccagcgg ttggccgccg
     4081 gcgtgcgcat cagttcgtac acgcgcgtat cgaagctggc ctggtcttcc acgctcacgc
     4141 ccagccagac gttgggcagc gtgggcagct gcgcgcgctg gaaatagcgg cgcatgtagc
     4201 ccgggttctt ggtcagcacg aagaagcggt ggtgcccggc gttcttcatc acggtgaaga
     4261 tgcgcgtgcg caggttgtcg tcgatctcgg cctggaacag gtcggactgc ggcgccacca
     4321 ggatatccat cgccttgcgc aggcggatcg ggtccatcag ctgcgtctcg ctggccaccg
     4381 gcatctcgct ggtggccgac gggcagtcat tgcacgccgc cgacgcgcgc agtcggcagc
     4441 ccttcaccgg gtgccactcg aagtcgaacg gcgacggcgg cggcgcgttg gccgcggctt
     4501 cggccttggc cgcggccttg gccatttcct cggcgcgcat ggtgcgcgac agcgactcca
     4561 gcagctcgtc gacttcggca gatgaggcct tgcgcgcagc agacgacggc gaggtggatc
     4621 ccatcgggat caactcggcc gacaggtcga caaccgctgc gttcagatcg ggatcaagcc
     4681 cggcgctcat caaaaaaccc tccttatccc cgcaggcccc cgcctgcctt ccggctgccg
     4741 cgccctctac ggagcagcac acagccgagc tagcaaccaa tgtaccaggc actgcaggat
     4801 caggccgtac tattcaacag tgcatggcgc gaaacaatgg taatttttta ccagaatcgc
     4861 gtttcgttta ccagttggcg aatggaatcc tggcgctgca cgtccatggt cccctccggc
     4921 ccgcccgacg gcgttggggg cacggcccgc ggctggctat actttgaccg gagaaggtta
     4981 cagggagcgg tcgtggcggg ggcatttctt cactgggtac tgcgcgtggc ggtcgtggcc
     5041 gtgctggttg ccggcggtgc cgccgctgcg gcgtcggacg acaaccccat ctacctgtac
     5101 cggcagagca acggcgtgcg cgtgtacacc gaccgcaagc ccgatcacag cgcctgggtc
     5161 atcatcgcca agtacggccg gcccaccgcg tcggcctcgt gcaccggcct gaccgcgcag
     5221 tcgctcgatg cccgcgcgtc cacctacgcc ccgctgatcg ccaagtacgc cagcgcgcgc
     5281 ggcgtgccgg cggcgctggt gcgcgcggtg atgcgggtgg aatcctgctt cgaccaccgt
     5341 gccgtttcgc gcgtcggtgc gcgcgggctg atgcagctga tgcccgaaac ggccgcggaa
     5401 atgggcgtaa gcgacagctt cgaccccgag cagaacattt ccggcggcgt gcactacctg
     5461 tcgatgatgc tggagcgatt ccaccagaac cgcgaactgg cgctggccgc ctacaacgcc
     5521 gggccggaag ccgtggaaaa gcatggcggc attccgccct tccccgagac gcagagctac
     5581 gtgcgccgcg tcatcgccag ctaccgccag cagacctgac tcggaactct cggccacatc
     5641 tgtagtctgt aggcagcgcc ggggaggcgc caaccacggg gagcacccat gaagtacacc
     5701 aggatcggca tggccgccgc gctgtgcggc atcgtcgtgt ctgcgcaggc cgcggcaccc
     5761 gcctgggtgg cggagagcaa ccgcaacacc gacgtgctgt tgcagcaaca ggcgcgcttc
     5821 aacccggaaa acgccagcgc gcagggcgtt gaaggctatg acgaccaggt cgtcgacctg
     5881 cagccaaagg tgtacgagcg cagtcaggcc gcggcggcgg acgcggtgaa ggaactgcag
     5941 cgccggctgg cggccgccag cgatccgcag gtgaagcagg acctggaaat cctcatcggc
     6001 tccgcgcagg acggcatcga gaccgcgcgg ctgaacaacg acgagctgct gccctacttc
     6061 agcgtggcgc gcatcgtgtt cagcggcatg cagagcctgc tcgacccccg cgtatcgaaa
//
LOCUS       DASZZC010000572         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_369796,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000572 DASZZC010000000
VERSION     DASZZC010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369796"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VHE37_12210"
     CDS             complement(<1..825)
                     /locus_tag="VHE37_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HVT36346.1"
                     /translation="MHSLRYRIARWVMQHRMVAWIVFAAITVFFAAGLRNVELRTIFS
                     DLLPADDPFVQVFKDHPNFGNPLTVTVMVKRKNGDIYNHETLAKVWKLTRDIDLTPGV
                     DHDQILSIATEKARYAEATPYGIDMRPLMEDRVPETAEEIAEFKGRVDKSPAARTFLI
                     SGDSSATLINATFIEDKLDYGVVFDFVHDLVEKASDADHEVYMAGQPTLTGWVYHYES
                     QTFSIFAVTIGALILALALYMRNVVGVVTPIVTSTVAAIWGFGFVGWLKAPVEPLLM"
     gene            complement(984..>1798)
                     /locus_tag="VHE37_12215"
     CDS             complement(984..>1798)
                     /locus_tag="VHE37_12215"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVT36347.1"
                     /translation="ARGRPAKALETLDEAESVGHTHGWERLLRLINWERVRRALVAGA
                     VERAQAIAALMPPGPASGERILFSEDAESETLSRIRLAIQRMDYDAAAHRLTAEFARG
                     RGRDYRQIKLHLLEAALHHARDERNAAHRSLRKALALAAPGGYVRCFLDEGDAVLQLL
                     REAYQHLLEAEDADACAGQRAHIELLLQASGTDLSRARPAREAAPVEALTDREREILV
                     FLANGVSNKEMAGRLFVSENTVKFHLKNIYSKLSVGSRLQAITAGRKLGLVQ"
BASE COUNT          267 a          611 c          606 g          314 t
ORIGIN      
        1 catcagcagc ggctccaccg gcgccttgag ccagccgacg aagccgaagc cccagatcgc
       61 cgccaccgtg ctggtgacga tgggcgtgac cacgccgacc acgttgcgca tgtacagcgc
      121 cagcgccagg atcagcgcgc cgatggtgac ggcgaagatg ctgaaggtct gcgactcgta
      181 gtggtagacc cagccggtca gcgtgggctg cccggccatg tacacctcgt ggtcggcgtc
      241 gctggccttc tccaccaagt catgcacgaa gtcgaacacc acgccgtaat cgagcttgtc
      301 ctcgatgaag gtggcgttga tcagcgtggc gctgctgtcg ccggagatca ggaaggtgcg
      361 cgccgcgggc gacttgtcga cgcggccctt gaactcggcg atctcctcgg cggtttccgg
      421 cacgcggtct tccatcagcg ggcgcatgtc gatgccgtag ggcgtggcct cggcgtagcg
      481 cgccttctcg gtggcgatcg agaggatctg gtcgtggtcg acacccggcg tgaggtcgat
      541 gtcgcgcgtc agcttccaga ctttcgccag cgtctcgtgg ttgtagatgt cgccgttctt
      601 ccgcttgacc atcacggtga cggtcagcgg gttgccgaag ttggggtggt ccttgaacac
      661 ctgcacgaac gggtcgtcgg ccggcagcag gtcggagaag atggtgcgca gctcgacatt
      721 gcgcaggccg gcggcgaaaa acacggtgat cgcggcgaaa acgatccacg ccaccatccg
      781 gtgctgcatg acccagcgcg caatccgata acgcaatgaa tgcatgccta cgctctccag
      841 tcgcctcgca gcatcgcccg ggtgcgcgtc gccgcgccgc gggttctagg tctgcgcagg
      901 attagaagcc ctgcgccgcc ttggggcact acacgaaagg gtagttttga tttcgcgcgg
      961 ctttttcccc tggcggccgc ggcttactgc accaatccca gttttctgcc ggccgtgatc
     1021 gcctgcaggc ggctgccgac cgaaagcttg gagtagatgt tcttcaggtg aaacttcacc
     1081 gtgttctccg acacgaacag gcgcccggcc atttccttgt tcgacacgcc gttggccagg
     1141 aacaccagga tctcccgctc gcggtcggtc aaggcctcca ccggcgccgc ttcgcgcgcg
     1201 ggtcgcgcgc gcgacaggtc agtgcccgag gcctgcagca gcaattcgat atgcgcgcgc
     1261 tggccggcgc aggcgtcggc gtcttcggct tcgagcaggt gctggtaggc ctcgcgcagc
     1321 aactgcagta cggcgtcacc ttcgtcgagg aagcagcgca cgtagccgcc cggcgccgcc
     1381 agtgccagcg ccttgcgcag gctgcggtgc gcggcgttgc gctcgtcgcg cgcgtggtgc
     1441 agcgccgcct ccagcaggtg cagcttgatc tgccggtagt cacgcccgcg cccgcgcgcg
     1501 aattccgccg tcagccggtg cgcggcggcg tcgtagtcca tgcgctggat ggccaggcgg
     1561 atgcgcgaca gcgtctcgct ctccgcgtcc tccgaaaaca ggatgcgctc gccgctcgcc
     1621 ggccccggcg gcatcagcgc ggcgatggcc tgtgcgcgct cgactgcgcc ggcgaccagc
     1681 gcgcggcgca cgcgctccca gttgatcagg cgcagcaggc gttcccagcc atgcgtgtgg
     1741 ccgacgctct cggcctcgtc gagcgtctcc agcgccttcg ccgggcgccc gcgcgcgt
//
LOCUS       DASZZC010000573         8114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_560155,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000573 DASZZC010000000
VERSION     DASZZC010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560155"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VHE37_12220"
     CDS             complement(<1..1555)
                     /locus_tag="VHE37_12220"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HVT36348.1"
                     /translation="MIARLRPAALLPAFLCLFVAACHNGGAGTSAPVTPPVNHPPHAD
                     AGPAQTVNSGTLVTLSGANSTDADGPIASYHWQQVSPAGDVGLATPNNVSTTFTAPTV
                     TADTVYTFRLTVTDGGNLSDGADVAITVRPAAAGNQPPVANAGADQVVNETASVTLDG
                     SASSDPDGTIQSYAWTQVSPATPQVTLTNADHAQATFTAPDVSADTPFTFRITVTDNS
                     GASSSDDVVVTVRDTTVPPQPELSVADASAGEGDGHIDFTITLSPAAAQPVTVQYATH
                     DGTATAGSDYVATSGTATFAAGATSATVTVPVLEDSVYEGNETFQLQLSQPTNATLHN
                     AAATGTITDDDPPPPVADDCAPGALCAGASKRAITPSTQHIAGVVEDRIGGTQVTQRF
                     HLGGFGFGPFEASKFLDQYSGGALSERTCIPGSDTCLSEEPASRAYNCLDGAATCAQD
                     SADAEQTWVRAFYLSQPAATGGDTELIFVTMDAVGAGNLIVGGVKNAVSAATGVPVDN
                     IIVGMTHSHA"
     gene            complement(1723..2331)
                     /locus_tag="VHE37_12225"
     CDS             complement(1723..2331)
                     /locus_tag="VHE37_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT36349.1"
                     /translation="MFKLYGMTGSGNCWKPAALMRQLQLPFQWIETDLLKGATRTPEF
                     LARNPNGKVPLLELEDGRRLAESNAMLCFLAEDTPYFPFDRWARAQVLQWLFFEQYSH
                     EPYIATVRFWVHFLHKRDEWKEKIAEAMAKGYAALGVMERQLATTPFLVGGEYTIADI
                     ALYAYTHVAHQGGYELARFPAVQAWLARCEAQPRFQPMEPRG"
     gene            complement(2437..3045)
                     /locus_tag="VHE37_12230"
     CDS             complement(2437..3045)
                     /locus_tag="VHE37_12230"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36350.1"
                     /translation="MTAATRRQPDLTRQKLLECAFEEMHASGFRSASLDTILQSAGVT
                     KGALYHHFASKADLGYAVVDEVIRPWIEENWKPAAQSDNPVDAAIALCRSLTQLRSDK
                     ALTCGCPFNNLINEMSPVDEGFRTRLHQILAEWRSGIAQSLAAAQARGLVRREVEPEA
                     AAAFVISAIEGCIGLAKADQSREFLEAGMRGLIDYLEQLRAR"
     gene            3138..4304
                     /locus_tag="VHE37_12235"
     CDS             3138..4304
                     /locus_tag="VHE37_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HVT36351.1"
                     /translation="MEQGYDTAALVRDLKSRVPEDAAALLARQAPERIAQALEMLPPD
                     LAARIAAQLPGALQAETAEIRVPQQISELMEPVHGVLPATTTVAQACDYLRHEDTPTE
                     VITYLYVADEGDRLLGLVLMRDLIVAVPGKTLREIMLGNPFHLLPQMKIGEAVKLAAR
                     RHYPVYPVCDERGHIIGIVRGWKLFERQAIEISAQSGSMVGVNKEERLATPVGPAFAQ
                     RHPWLQVNLLTAFLDVFVVGLFSGTIEKIVALAAFLPLLAGQSGNTGCQALAITLRGM
                     TLGDAEGYPKKRIFMKELSLGLLNGVFTGLVAAGAMYWYASPSPDAFKLALTIWLAMV
                     GGCIVAGLAGVAIPLLLKRFGADPVTASAIFLTTCTDIAGMGLMLVLATVIVLH"
     gene            4309..5046
                     /locus_tag="VHE37_12240"
     CDS             4309..5046
                     /locus_tag="VHE37_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT36352.1"
                     /translation="MPLTLAVAVASYFTALLSGVAGLGGGTVLIGIFYAIGLAPAIAV
                     PLHAAVQLISNASRTLAYLKFVQWRAALWFLVGALPGPFLIAPWVQQANPYALDLLMA
                     LLVGASLLPEKKSAVQLPTWAAILCAGLLNGSLGMFIGATGLVIGRLFLRPEWRKESV
                     IGTLALCQSLGHASKIAGFATLGLSALARIDILAPLAIATVLGTLSGRWLHRYVSEAL
                     FQKVFKSILAVLAVKLAYDGLSGLPWH"
     gene            5037..5459
                     /locus_tag="VHE37_12245"
     CDS             5037..5459
                     /locus_tag="VHE37_12245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVT36353.1"
                     /translation="MALKMTAQQVEDLIHLGFASKRKFMQCEEVRENYARVRLPYRDT
                     MLRPGGTLSGPTLFTAADIAMYALVLSHIGPELMAVTSNMNMNFLARPKAGDAIAEAK
                     LLRLGRRQVVMEVSLYSSAGPEVLVAHVTGTYALPIKS"
     gene            complement(5568..6155)
                     /locus_tag="VHE37_12250"
     CDS             complement(5568..6155)
                     /locus_tag="VHE37_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36354.1"
                     /translation="MNKHSVLILTVLALCACGKHEAPAPQPEAPKMAEHVDAVPQTTP
                     AESAQPAEAPPAINGEEVSINEPMPPVDEEGAPPIITAQGKCQGAAGATVSGDVTIYD
                     AGSGSRLLRIENLKSPGGTTDIGLVTSNAPTTADLSAIATLGSLKGASGNMNYLVDRK
                     LALSPPRALALAAHDRHELIGWCSLVPPKADAGKK"
     gene            complement(6160..7470)
                     /locus_tag="VHE37_12255"
     CDS             complement(6160..7470)
                     /locus_tag="VHE37_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT36355.1"
                     /translation="MLSLSRYQWTVLFAAWLGWGFDVFDGLLFNYVAPNCVPTLLHLQ
                     IGSPEAKQATLYWTGLLTSMLLVGWAIGGILFGWICDRIGRTRTLLLTMLMYAGGTAA
                     CAAADSMTMLLAFRLIASFGIGGEWAAGASMVAEVMPENKRVEAGALLYTSAPFGLFL
                     ATFVNYRIAGDWFRDDPENSWRYVFLCGLLPVAAALAVRIFLKEPERWQQAAQTQAPP
                     SIAELFTPKYRAITLSGFLMSFVALISWWSCNAFIPVVSSGLAQAAAKAQNLDKAGTL
                     ALVEAWKKLATNYFNVGGLIGTLLTIPAAKLLGRRQMFLIYYSGAALALFATFGLDLP
                     DELRLKMYFVVGLTVFGVFGSFTYYLPELFPTRLRGTGAGFCYNVGRIVASVGPFMVG
                     AIAAQGANAANSAIHVLMYVGFAPVLALVFMPWVIETRGRPLPE"
     gene            complement(7641..>8114)
                     /locus_tag="VHE37_12260"
     CDS             complement(7641..>8114)
                     /locus_tag="VHE37_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HVT36356.1"
                     /translation="PRVEAETRRFIEEELAAQAALDLASLPQGVIHADLFKDNVLFVD
                     DRLSGLIDFYYACNDALLYDVAVTLNDWCFEPDGRYNGARAQALLTAYRGSREFTEAE
                     LAAWPLVLRAAAFRFYLSRLYDWVFPRSGDVVHVKDPAQYRRVLEHHRAHAAPAL"
BASE COUNT         1267 a         2666 c         2794 g         1387 t
ORIGIN      
        1 cggcatgcga gtgcgtcatg ccgacgatga tgttgtccac cggcacgccg gtggcggcac
       61 tgacggcatt cttcacgccg cccacgatga ggttgccggc gcccaccgca tccatggtga
      121 cgaagatcag ctcggtgtcg ccgccggttg cagccggctg cgacagatag aacgcgcgca
      181 cccaggtctg ctcggcgtcg gcgctgtcct gcgcgcacgt cgctgcgccg tccaggcagt
      241 tataggcgcg gctggccggc tcttcggaca ggcaggtgtc ggaaccggga atgcaggtac
      301 gttccgacag cgcgcctccg gagtactggt ccaggaactt cgacgcctcg aacgggccga
      361 agccgaagcc gcccaggtgg aagcgctgtg tcacctgcgt gccgccgatg cggtcttcca
      421 ccacgccggc gatgtgctgc gtgctcggcg tgatggcgcg cttggatgcg ccggcgcaca
      481 gcgcaccggg cgcgcagtcg tcggccaccg gcggcggcgg atcgtcatcg gtgatggtgc
      541 cggtggcggc ggcgttgtgc agcgtggcat tggtcggttg cgacagctgc agctggaagg
      601 tctcgttgcc ctcgtagacg ctgtcctcca gcaccggcac ggtcacggtg gcgctggtgg
      661 cgccggcggc gaacgtggca gtgccgctgg tggcaacgta atcgctgccg gcggtagcgg
      721 tgccgtcgtg cgtggcgtac tgcacggtca ccggctgcgc agcggccggc gacagcgtga
      781 tggtgaagtc gatatgcccg tcgccttcgc ccgcgctggc atcggccacg ctcagctccg
      841 gctgcggcgg caccgtggtg tcgcgcacgg tcaccaccac gtcgtcgctg ctgctggcgc
      901 cgctgttgtc ggtgacggta atgcggaaag tgaagggcgt gtccgcactc acatcgggtg
      961 cggtgaacgt cgcctgcgca tggtcggcgt tggtcagtgt cacctgcggc gtggccggcg
     1021 acacctgcgt ccatgcatat gactgaatgg ttccatcggg atcgctgctg gcgctgccgt
     1081 cgagggtgac ggacgcggtt tcgttcacca cctggtcggc gccggcgttg gccaccggcg
     1141 gctggttgcc ggccgcggcg ggccgcaccg tgatcgccac gtcggcgccg tcgctcaggt
     1201 tgccgccgtc ggtcacggtg aggcggaagg tgtagacggt atccgcggtc accgtgggcg
     1261 cggtgaaggt ggtgctgacg ttgttcggcg tcgcaagacc cacatcacct gcaggtgaaa
     1321 cctgctgcca gtgatagctg gcgatggggc cgtcggcatc ggtgctgttg gcgccactga
     1381 gtgtgaccag cgtgccgctg ttcaccgtct gcgcggggcc ggcatccgca tgcggcggat
     1441 gattgaccgg cggcgtcacg ggcgcggaag tgccggctcc gccgttgtgg caggcggcaa
     1501 cgaacagaca caggaatgca ggcagcaagg ctgccggacg caggcgcgcg atcatcgtat
     1561 ggctctcctc aatagcggcg ctcggggccg ctgatccctg atggaggctg tatgcctctc
     1621 tagttgagtg caggaattct agtgagggag ccgccggggt gtatgggtaa attcccctct
     1681 agtacgctag agaaaatctc ccgtccttca ccgcccgctg cttcagccgc gcggctccat
     1741 cggctggaag cgcggctgcg cctcgcagcg cgccagccac gcctgcaccg cggggaaccg
     1801 cgcgagctcg tagccgccct gatgcgcgac atgcgtgtac gcgtacagcg cgatgtcggc
     1861 gatggtgtac tccccgccga ccagaaacgg cgtggttgcc agctgccgtt ccatcacgcc
     1921 cagcgcggcg tagcccttgg ccatcgcttc ggcgatcttc tccttccact cgtcgcgctt
     1981 gtgcagaaag tgcacccaga agcgcacggt ggcgatgtac ggctcgtggc tgtactgctc
     2041 gaagaacagc cactgcagca cctgcgcgcg ggcccagcgg tcgaacggaa aatagggtgt
     2101 gtcctcggcc aggaagcaca gcatggcgtt ggactcggcc agcctgcggc catcttcgag
     2161 ttccagcagc ggcaccttgc cgttgggatt gcgcgccagg aattccggcg tgcgcgtggc
     2221 gcccttgagc agatcggtct cgatccactg gaacggcagc tgcagctggc gcatcagcgc
     2281 cgccggcttc cagcaattgc cggagccggt catgccatag agcttgaaca tgcgggcgtc
     2341 tcccggattg catgcatggt ccgcgcgcat tgtccgcgcg cggcgcgacg gacctcaagc
     2401 gaaatgcgcg catcgcccgc atgcgcggac ggcatgtcag cgcgcgcgca gctgttccag
     2461 gtaatcgatc agcccgcgca tgccggcttc gaggaactcg cgcgactggt cggccttggc
     2521 cagcccgatg cagccctcga tggcgctgat gacgaacgcg gcggcggctt ccggctcgac
     2581 ctcgcgccgc accagtccgc gcgcctgcgc ggcggccagc gactgcgcga tgccgctgcg
     2641 ccattcggcc aggatctggt gcagccgcgt gcgaaagcct tcgtccaccg gcgacatttc
     2701 attgatcagg ttgttgaacg ggcagccgca ggtcagcgcc ttgtccgagc gcagctgggt
     2761 gaggctgcgg cacagcgcga tggcggcgtc gaccgggttg tcggactgcg ccgccggctt
     2821 ccagttttcc tcgatccacg ggcggatcac ctcgtccacc accgcgtagc ccaggtcggc
     2881 cttgctggca aagtggtggt acagcgcacc cttggtcacg ccggccgatt gcaggatggt
     2941 gtcgaggctg gcgctgcgga atccgctggc gtgcatttcc tcgaacgcgc actcgagcag
     3001 cttctggcgc gtgaggtccg gctggcggcg ggtggcggcg gtcatgagtg gcggagtatg
     3061 tgcgcgcacg tgggcttgtc aatcggcgct gcggtggcgg cggcataatg catggcagcc
     3121 gaagcaggga gcgaatcatg gagcagggct acgacaccgc cgcgctggtg cgcgatctca
     3181 agtcccgcgt gccggaagat gccgccgcgc tgctcgcgcg gcaggcgccc gagcgcatcg
     3241 cgcaggcgct ggagatgctg ccgccagacc tggccgcgcg catcgcggcg cagctgcccg
     3301 gcgcgctgca ggccgagacc gccgagatca gggtgccgca gcagatctcc gagctgatgg
     3361 agccggtgca cggcgtgctg ccggcgacca ccaccgtcgc gcaggcctgc gattacctgc
     3421 gccacgagga cacacccacc gaggtcatca cctacctgta cgtggccgac gaaggcgacc
     3481 gcctgctcgg cctggtgctg atgcgcgacc tgatcgtcgc cgtgccgggc aagaccctgc
     3541 gcgagatcat gctgggcaat ccgttccacc tgctgccgca gatgaagatc ggcgaggcgg
     3601 tgaagctcgc cgcgcggcgc cactacccgg tgtacccggt gtgcgacgag cgcgggcaca
     3661 tcatcggcat cgtgcgcggc tggaagctgt tcgagcggca ggcgatcgaa atctccgcgc
     3721 agtcgggcag catggtcggc gtgaacaagg aggaacggct ggccacgccg gtcggcccgg
     3781 ccttcgcgca gcgccacccg tggctgcagg tgaacctgct caccgctttc ctcgatgtgt
     3841 tcgtggtcgg cttgttctcc ggcaccatcg agaagatcgt cgcgctcgcc gccttcctgc
     3901 cgctgctcgc cgggcagtcc ggcaacaccg gctgccaggc gctggccatc accctgcgcg
     3961 gcatgacgct gggcgatgcc gagggctatc cgaagaaacg gatcttcatg aaggaactga
     4021 gcctgggcct gctcaacggc gtgtttaccg ggctggtcgc ggccggcgcc atgtactggt
     4081 atgcctcgcc cagccccgac gcattcaagc tcgcgctgac catctggctg gccatggtcg
     4141 gcggctgcat cgtcgcgggc ctggcgggcg tggccatccc gctgctgctc aagcgcttcg
     4201 gcgccgaccc ggtgaccgcg tcagcgatct ttctcacgac atgtaccgat atcgcgggca
     4261 tggggttgat gctcgtgctg gcgaccgtga tcgtgctgca ttgacctgat gccgctgaca
     4321 ctcgcggtcg ccgtcgcctc ttacttcacc gcactgctgt ccggcgtggc cgggctcggc
     4381 ggcggcacgg tgctgatcgg catcttctac gccatcggcc tcgcgccagc catcgcggtg
     4441 ccgctgcatg cggcggtgca gctgatctcc aacgcgtcgc gcacgctggc ctatctcaag
     4501 ttcgtgcagt ggcgcgcggc gctgtggttc ctggtcggcg cgctgcccgg tccgttcctg
     4561 atcgcgccat gggtgcagca ggccaacccc tatgcgctcg acctgctgat ggcgttgctg
     4621 gtcggcgcct cgctgctgcc ggagaaaaaa tccgcggtgc agctgccgac gtgggccgcc
     4681 atcctgtgcg ccggcctgct caacggttcg ctcggcatgt tcatcggcgc caccggcctg
     4741 gtcatcggcc ggctgttcct gcgaccggag tggcgcaagg aaagcgtgat cggcacgctg
     4801 gcgctgtgcc agtcgctggg ccacgcgagc aagatcgccg gcttcgccac gctagggctg
     4861 tcggcgctgg cgcgcatcga catcctcgcg ccgctggcca tcgccaccgt gctcggcacg
     4921 ctgtccgggc gctggctgca tcgctacgtg agcgaggcgc tgttccagaa agtgttcaag
     4981 tcgatactcg ccgtgctcgc ggtgaagctg gcttatgacg gactttcggg attgccatgg
     5041 cactgaagat gaccgcgcag caggtggaag acctgatcca cctgggcttc gcctcgaaaa
     5101 gaaaattcat gcagtgcgaa gaggtgcgcg agaactacgc gcgcgtgcgc ctgccgtacc
     5161 gcgacaccat gctgcgcccc ggtggcacgc tgagcgggcc tacgctgttc accgccgccg
     5221 acatcgccat gtatgcgctg gtgctgtcgc acatcgggcc cgagctgatg gcggtgacct
     5281 cgaacatgaa catgaatttc ctggcgcggc cgaaagccgg cgatgccatc gccgaagcga
     5341 agctgctgcg gctgggcaga cggcaggtgg tgatggaagt gtcgctgtat tcgtcggccg
     5401 ggcccgaggt gctggtggcg catgtgaccg gcacctacgc gctgccgatc aagtcctgaa
     5461 gcgcgatttc tgtctccacc gcacctcgat tccttgtgtt atttattatt aagcaattat
     5521 aaattaatac aatattttat aaccgagaat ccggcagtgc ggtcagctca ttttttgccc
     5581 gcatcggcct tgggcggcac cagcgagcac cagccgatca actcatgcct gtcgtgcgcc
     5641 gccagcgcca gtgcgcgcgg cggcgacagc gcaagcttgc ggtcgaccag gtagttcatg
     5701 ttgccgctgg cgcccttgag cgaaccgagg gttgcgatcg ccgacaggtc cgcggtggtc
     5761 ggcgcattgc tcgtcaccag cccgatgtca gtggtgccgc cgggcgactt caggttctcg
     5821 atgcgcagca ggcggctgcc ggagccggcg tcgtagatgg tgacgtcgcc gctgaccgtc
     5881 gcgccggccg cgccctggca cttgccttgc gcggtgatga tcggcggggc gccttcctcg
     5941 tcgaccggcg gcatcggctc gttgatcgat acttcctcgc cgttgatcgc gggcggcgcc
     6001 tcggcgggct gcgccgactc tgccggtgtg gtctgcggca ccgcgtcgac atgctcggcc
     6061 atcttcggcg cttccggctg cggcgcgggc gcttcgtgct tgccgcaggc gcacaacgcg
     6121 agaacggtca agatcaaaac agaatgcttg ttcatttttc tactcgggca agggacggcc
     6181 gcgggtttcg atcacccagg gcatgaagac cagcgccagc accggcgcga agccgacgta
     6241 catcagcacg tgaatggcgg agttcgccgc gttggcgccc tgcgcggcga tggcgccgac
     6301 catgaacggc ccgaccgatg cgacgatgcg gccgacgttg tagcagaaac cggcgccggt
     6361 gccgcgcagg cgcgtgggga acagttccgg caggtaatag gtgaagctgc cgaagacgcc
     6421 gaatacggtc agcccgacca cgaaatacat cttcagccgc agctcgtcgg gcaggtcgag
     6481 accgaaggtg gcgaacagcg ccagcgccgc gccgctgtag tagatgagga acatctgcct
     6541 gcgcccgagc agcttcgccg ccgggatggt gagcagcgtg ccgatcaggc caccgacgtt
     6601 gaagtagttg gtggccagct tcttccacgc ctccaccagt gccagcgtgc cggccttgtc
     6661 caggttctgc gccttggccg ctgcctgcgc cagcccgctc gacaccaccg ggatgaaggc
     6721 gttgcagctc caccaggaaa tcagcgcgac gaagctcatc aggaatcccg acagcgtgat
     6781 ggcgcggtat ttcggtgtga acagttcggc gatcgatggc ggcgcctgcg tctgcgccgc
     6841 ctgctgccag cgctccggct ccttgaggaa gatgcgcacc gccagcgccg cggccaccgg
     6901 cagcaggccg cacaggaaca cgtagcgcca ggaattttcc gggtcgtccc tgaaccagtc
     6961 gccggcgatc cggtagttga cgaaggtggc gaggaacagg ccgaacggcg ccgaggtgta
     7021 gagcagcgca ccggcttcca cgcgcttgtt ctccggcatt acctccgcaa ccatcgaggc
     7081 gcccgcggcc cattcgccgc cgatgccaaa gctggcaatg aggcggaacg ccagcagcat
     7141 ggtcatgctg tccgctgctg cgcaggccgc ggtgccgccg gcgtacatca gcatggtcag
     7201 cagcagcgtg cgcgtgcggc cgatgcggtc gcagatccag ccgaacagga tgccgccgat
     7261 ggcccagccc accagcagca tcgaggtgag cagcccggtc cagtacagcg tggcctgctt
     7321 ggcctccggc gagccgatct gcaggtgcag cagcgtgggc acgcagttgg gcgccacgta
     7381 gttgaacagc aggccgtcga acacgtcgaa gccccagccg agccaggcgg cgaacagcac
     7441 cgtccattgg tagcgggaca aactcaacat cgcgctctcc ccgtttttta aattctaagg
     7501 aaactttgaa gaagcgtcgc gagcgacgac aggttgcgcg aggacggagc gcgggaaccg
     7561 gagcatacac gccagtatgt gaggattccg ggcaccgccc tcgcgcaagc tgtcgagcgc
     7621 agcagcctgt gcaaagtttc ctacagcgcg ggcgccgcgt gcgcgcggtg gtgttcgagc
     7681 acgcgccggt actgcgccgg gtctttcacg tgcaccacgt cgccgctgcg cgggaacacc
     7741 cagtcgtaca ggcgcgagag atagaagcgg aacgcggccg cgcgcagcac cagcggccac
     7801 gctgccagtt ccgcttcggt gaattcgcgg ctgccgcggt atgcggtcag cagcgcctgc
     7861 gcgcgcgcgc cgttgtagcg gccgtcgggc tcgaagcacc agtcgttgag cgtcaccgcc
     7921 acgtcgtaca gcagcgcgtc gttgcaggcg tagtagaagt cgatcaggcc ggacaggcgg
     7981 tcgtcgacga acagcacgtt gtccttgaac aggtcggcgt ggatcacgcc ctgcggcaac
     8041 gacgccaggt cgagcgcagc ctgcgccgcc agctcctcct cgatgaaacg gcgcgtctcc
     8101 gcctccacgc gcgg
//
LOCUS       DASZZC010000574         6982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1072616,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000574 DASZZC010000000
VERSION     DASZZC010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072616"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="VHE37_12265"
     CDS             complement(<1..1782)
                     /locus_tag="VHE37_12265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVT36357.1"
                     /translation="MNTFAADDAALPASNPFAAPSTLPYQLPPFDKIGNADFKPAMEA
                     GIAQESRDFDAIAKNPDAPTFDNTIVAMERAGDLLNRVTTVFYNLTASNTNDDLEALN
                     TEMAPRLSAHNDDILLNPALFARVNALYEKRDQLGLDAESRQALERRYMTFVLAGAKL
                     SEADKVKLKGYNEELSKLTTQFQQNVLKSTRDNAVVVDNAAELDGLSAEQIGAAAEAA
                     KARKLDGKWVLTLSNTTTQPLLAQLKNRALREKLYRASISRSNGGPADNRPVVAQMVK
                     IRAERAALLGFPNHAASVLVDEGAGTPAAVDKMLHDLAPAAMANEKQEAAELQALIDR
                     QAKAAGSKPFKLQPWDWQFYAEQVRKAKYAYDEADIKPYFELNHVLQDGVFYTAHELY
                     GLSFKERHDLPVYQKDVRVFEVFNADGSPLGLFLADYFARDNKQGGAWMNEYVTQSHL
                     LGKKAVVINNLNIPKPADGQPALLTFDEVTTMFHEFGHGLHGLFSDVQYPSLAGTNVP
                     RDFVEYPSQYNEMWAAEPAVLKNYARHYQTGAPLPQALLDKVLAAQKFGEGFATGEYI
                     AAAMVDMSWHEITPAQAPPADKVMAFEA"
     gene            complement(2056..2508)
                     /locus_tag="VHE37_12270"
     CDS             complement(2056..2508)
                     /locus_tag="VHE37_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVT36358.1"
                     /translation="MKTLLLVAHAPSPNTQRLRDALVEGANVDGVALRALTPFETGPD
                     DVLAADGVLLFTPENLGYMSGALKDFFDRIYYPTLERKQGLPYALAIRAGNDGRGAQR
                     AVQTIVTGLRWREVSDTLICRGEFQQPWLAQCAEFAAGFAVGLEAGVW"
     gene            complement(2505..3665)
                     /locus_tag="VHE37_12275"
     CDS             complement(2505..3665)
                     /locus_tag="VHE37_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HVT36359.1"
                     /translation="MKNNTRGVSQGWLRAGLLLLGWAWAVQGAYAGDASAKMPGQVSL
                     SGTEFLRDGQPWVPRGVGITGLVLVDKLVASGKGRPPLIASHNMFGPALLDAVKAYGA
                     DSVRVAVSQPGLNPSDPLYDPGYADEVAQKIRLMRQRGLVVFVFMQWEPGTGSHNQSG
                     LTSDPATLAAWHTLLHKLPLDDHGIVLEPLNEPNHRGEQGAAPGPQVWASWQSGMQKL
                     VNAIRGEGWSGVMLIDGAYGSSWLDGAPAISDPQKPSQLAWSVHVYFTKSFRFVSQPD
                     WDQHFGDTCRAAACVVTEFQAARHTDCSRKGNNREMAEALVRYLKNRRIGLMPWAFDY
                     ENTMMEGHSLSQTTRYPKIAKCNKDGDDEGENFGNGELVHQYFTSGRVPEGG"
     gene            complement(3750..4790)
                     /locus_tag="VHE37_12280"
     CDS             complement(3750..4790)
                     /locus_tag="VHE37_12280"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVT36360.1"
                     /translation="MQPAQMPAATPESRDIITPYAFHVHPQLLGLPLARPWQRGLAIA
                     LDGLAVAMLSEVPGGGLVVVTAIVLFRWAGRTRVAAVSHWRGALRWLIAAGALLGLID
                     LTGAIRHEIREGGDVHARAAVVKHSAALAELLAAIESGRCDRVDCARGPVLDFAPVAR
                     KQGWSEQESLKKIDELLDDLNLGKADLASLHEQFHQRFVDPSVQPTAAASALPKLPDP
                     IEVGDDRPRYSIIGWAKGILDDLGIGLGWSALYFSAFTAWWRGQTPGKKLVGIRAVRL
                     DGKPFTLWTSFERYGGYGAGFATGLLGFLQVFWDRNRQAIHDKISETVVIVGDVPRDM
                     VPEAAGAVPGIG"
     gene            5051..6265
                     /locus_tag="VHE37_12285"
     CDS             5051..6265
                     /locus_tag="VHE37_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36361.1"
                     /translation="MAATLPASAWAFGVDEAEVLSRYGAPLHARVPISLGDGDRIEGP
                     DELSVRVLRTEGSADTPYSYSEDSIEASVAGSGRDLWVELRGKRPLREPLLLLQLEVR
                     SGYTRLTRELPLMLDPPETSAPSAPLRARAVAEAPLPVLSPAPAPVAAIALTPAPPAA
                     ESVAEPSHAHTSASRRMHHPRARAAAAQGEDRGLHLAGWNAQSYTQATQPLPMFQLDE
                     HFASLSELSPAPEPADAMAAAAAPVAPAATQAGGAAPAAAPAPAIAPALTPPADTQPS
                     SRGLWWWLLAVGLVAAYAVRRMRLAPRAVPPSRRARGVAGSDAARAEAPVPAAEAAHS
                     AVEDATQPLRRRMAELARNIPSTDTAGLRKLQLVEAYLDLGKLAQAQAMLSELEAEKI
                     RTERPAITLVKG"
     gene            6272..>6982
                     /locus_tag="VHE37_12290"
     CDS             6272..>6982
                     /locus_tag="VHE37_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36362.1"
                     /translation="MKSIGAALLRSGAARAGAAMALAVTSCAAAAAPSPSPADTYLLP
                     IVPAHHYTPHLKLAGDFASLAARPAPVAASPAANKSAVSSPTADEVRAFNGTQVGVGS
                     TSWPEAGPHKPVATGGTDGPEAQGNWRSGSLLSVLIVRASSASRPGLVLGAALIVGLL
                     VLRGASSGGGTRRKLRDRSPRDAMTAAEELPSAKVHALEPAERLNFDMTRNQARGEPA
                     TAAGAADTMELRKRASAHW"
BASE COUNT         1074 a         2450 c         2309 g         1149 t
ORIGIN      
        1 ggcctcgaac gccatcacct tgtcggccgg cggcgcttgc gccggcgtga tctcgtgcca
       61 ggacatgtcg accatggccg cggcgatgta ctcgccggtg gcaaagccct cgccgaattt
      121 ctgcgcagcc agcaccttgt ccagcagcgc ctgcggcagc ggggcgccgg tctggtagtg
      181 cctggcgtag ttcttcagca ccgccggctc ggccgcccac atttcgttgt actgcgacgg
      241 gtactcgacg aagtcgcgcg gcacgttggt gccggccagc gacgggtact gcacgtccga
      301 gaacagcccg tgcaggccgt ggccgaactc gtggaacatg gtggtcacct cgtcgaaggt
      361 gagcagcgcc ggctggccgt cggccggctt ggggatgttg aggttgttga tcaccaccgc
      421 cttcttgccc agcaggtgcg actgggtgac gtactcgttc atccacgccc cgccctgctt
      481 gttgtcgcgc gcgaaatagt cggccaggaa caggcccagc ggcgagccgt cggcgttgaa
      541 cacctcgaac acgcgcacgt ccttctggta cacgggcagg tcgtgacgct ccttgaagct
      601 caagccgtac agttcgtgcg cggtgtagaa cacgccgtcc tgcagcacgt ggttgagctc
      661 gaagtacggc ttgatgtcgg cctcgtcgta ggcgtacttg gccttgcgca cctgctcggc
      721 atagaactgc cagtcccacg gctgcagctt gaacggcttg ctgccggcgg ccttggcctg
      781 gcgatcgatc agcgcctgca gctcggcggc ttcctgcttc tcgttggcca tcgccgccgg
      841 cgccaggtcg tgcagcatct tgtccaccgc cgccggcgtg ccggcgccct cgtccaccag
      901 caccgacgcc gcgtggttgg ggaagcccag cagcgcagcg cgctcggcgc ggatcttcac
      961 catctgcgcg accaccgggc ggttgtccgc cgggccgccg ttgctgcgcg atatcgacgc
     1021 ccggtacagc ttctcgcgca gtgcgcggtt cttcagctgc gccagcaacg gctgcgtcgt
     1081 cgtgttggac agcgtcagca cccacttgcc gtcaagcttg cgcgccttgg ccgcctcggc
     1141 cgcggcgccg atctgctcgg ccgacaggcc gtcgagctcg gccgcgttgt cgaccaccac
     1201 cgcgttgtcc ctggtcgact tgagcacgtt ctgctggaac tgcgtggtca gcttcgacag
     1261 ttcctcgttg tagcccttga gcttgacctt gtcggcttcc gacagcttgg cgccggccag
     1321 cacgaaggtc atgtagcggc gctccagcgc ctggcgcgat tccgcgtcca gcccgagctg
     1381 gtcgcgtttc tcgtacagcg cgttcacccg cgcgaacagg gccgggttga gcaggatgtc
     1441 gtcgttgtga gcggacagcc tgggcgccat ctcggtgttg agcgcttcca ggtcgtcgtt
     1501 ggtgttggag gcggtcaggt tgtagaacac cgtggtcacc cggtttagca ggtcgccggc
     1561 gcgctccatg gcgacgatgg tgttgtcgaa ggtcggcgcg tccggattct tggcgatggc
     1621 gtcgaaatcg cggctctcct gcgcaatgcc cgcttccatt gccggcttga agtcggcgtt
     1681 gccgatcttg tcgaacggcg gcagctggta cggcaatgtg ctgggcgcgg caaacggatt
     1741 gctcgccggc aaggcggcat cgtcagcggc gaatgtgttc atggcgaatg cgaccgcgca
     1801 ggcggccaac gctcctggca aatgtctcat gtgtctcccc gatggaagtc tggatgccgg
     1861 gcaccgcgct gtacccaccc gaaaaattcc cggcgaatgc ccgcagaccg cgcgccggca
     1921 aaaaagttcg gcgccggaag tcccaccgca ctgcgcgata ccgtgttatt aaatatatat
     1981 ctattttaaa tataataaat aattagtaac acgctaaacc caggtgcggt caaggccagg
     2041 tcggccattg cgggctcacc acacgcccgc ctccaggccg acggcgaacc ccgccgcaaa
     2101 ttccgcgcat tgcgccagcc acggctgctg gaactcgccg cggcagatca gcgtgtcgct
     2161 cacttcgcgc cagcgcagcc cggtgacgat ggtctgcacc gcgcgctgcg cgccgcggcc
     2221 atcatttccg gcgcggatcg ccagcgcgta aggcagaccc tgcttgcgct ccagcgtcgg
     2281 gtagtagatg cggtcgaaga aatccttcag cgcgccactc atgtagccca ggttctcggg
     2341 cgtgaacagc agcacgccgt cggccgcgag cacatcgtcc gggccggtct cgaacggcgt
     2401 gagcgcgcgc aacgcgactc catccacatt cgcgccttcg accagcgcat cgcgcaggcg
     2461 ctgcgtgttg ggcgatggcg catgcgccac cagcagcagg gtcttcaccc gccttccggc
     2521 acccgcccgc tggtgaagta ctggtgcacc agttcgccgt tgccgaagtt ttcgccttcg
     2581 tcatcgccgt ccttgttgca cttggcgatc ttcgggtagc gcgtggtctg gctcaggctg
     2641 tggccttcca tcatggtgtt ctcgtagtca aaggcccacg gcatcaggcc gatcctgcgg
     2701 ttcttcaggt agcgcaccag cgcctccgcc atctcgcggt tgttgccctt gcgcgagcag
     2761 tcggtgtggc gcgcggcctg gaactcggtg accacgcagg cggcggcgcg gcaggtgtcg
     2821 ccgaaatgct ggtcccagtc cggctggctg acgaagcgga atgacttggt gaaatacacg
     2881 tgcaccgacc aggccagctg cgacggcttc tgcggatcgg aaatcgccgg cgcgccgtcg
     2941 agccacgagg agccataggc gccgtcgatc agcatcacgc cactccagcc ttcgccgcgg
     3001 atggcattga ccagcttctg catgcccgac tgccagctgg cccacacctg cggccccggc
     3061 gccgcgccct gctcgccgcg atggttgggc tcgttcagcg gctccagcac gatgccgtga
     3121 tcgtccagcg gcagcttgtg cagcagcgtg tgccacgccg ccagcgtcgc cgggtcggac
     3181 gtgagcccgc tctggttgtg cgaaccggtg ccgggttccc actgcatgaa cacgaacacc
     3241 accaggccgc gctggcgcat caggcggatc ttctgcgcca cctcatcggc ataaccgggg
     3301 tcgtacagcg gatcggatgg attgagcccg ggctgcgaca ccgccacgcg caccgaatcg
     3361 gcgccgtagg ccttcaccgc atcgagcagc gcgggaccga acatgttgtg tgaagcgatc
     3421 agcggcggcc gccccttgcc cgaggccacc agcttgtcga ccagcaccag cccggtgatg
     3481 cccacgccgc gcggaaccca tggctgccca tcgcgcagga attcggtgcc cgacaggctc
     3541 acctgccccg gcatcttcgc ggacgcatcg ccggcgtatg cgccctgcac ggcccaggcc
     3601 catccgagca gcagcaggcc cgcgcgaagc catccttggg aaacaccccg cgtgttgttc
     3661 ttcatggctg caagtccttt tgttttgggt tgtgcgaatc ggatcgatgt acaccgctga
     3721 tgttgaacgg cagctgactg gcgacgccgc tagccgatgc ccggaaccgc gcccgccgct
     3781 tccggcacca tgtcgcgcgg tacgtcgccc acgatcacca cggtctcgga aatcttgtcg
     3841 tggatggcct ggcggttgcg gtcccagaac acctgcagga agccgagcag cccggtggcg
     3901 aagcccgcac cgtagccgcc gtagcgctcg aacgacgtcc acagcgtgaa cggtttgccg
     3961 tcgagccgca cggcgcggat gccgaccagc ttcttgcccg gcgtctgccc gcgccaccac
     4021 gcggtgaacg cgctgaagta cagcgccgac cagccgagcc cgatgccgag atcgtcgagg
     4081 atgcccttgg cccagccgat gatgctgtag cgcgggcggt cgtcaccgac ttcgatcgga
     4141 tcgggcaact tgggcagcgc ggaggccgcg gcggtcggct gcaccgatgg atccacgaag
     4201 cgctggtgga actgctcgtg caggctcgcg agatcggcct tgcccaggtt caggtcatcg
     4261 agcaattcgt cgatcttctt cagtgattcc tgctcgctcc agccctgctt gcgcgccacc
     4321 ggcgcgaaat ccagcaccgg gccgcgtgcg cagtcgaccc ggtcgcagcg cccgctctcg
     4381 atggcggcca gcagttccgc cagcgccgcc gaatgcttta ccactgcggc cctggcgtgc
     4441 acatcaccgc cctcgcggat ctcgtggcgg atggcgccgg tcaggtcgat cagtccgagc
     4501 agggcaccgg cggcgatcag ccagcgcagc gcgccgcgcc agtgcgacac ggcggccacg
     4561 cgcgtgcgcc cggcccagcg gaacagcacg atggccgtga ccaccaccaa tccgccgcca
     4621 ggcacttccg acagcatggc caccgccagg ccgtcgagcg caatggccag cccgcgctgc
     4681 cagggcctgg ccagcggcag gcccagtaat tgcggatgca cgtggaacgc gtacggcgtg
     4741 atgatgtcgc gtgattcggg cgtcgccgct ggcatttgcg caggctgcat ggagaagtcg
     4801 gtggcgggcg ggtcatctta aacaatccgc gccgccgcac gccgcatcca ggcggcgatt
     4861 ttccgcaagg cgcggctacc tcaaccggct ccgtgaaaca atgaggtctt acccactgcc
     4921 catgggatag cgcagccgtt ataaatcgcg cgcgattctg ggagaaaggg aaatgtcgtt
     4981 gcacggaagc cgatccggcc cggcggtagc cgcgggctgg ccggttcgtt gtgccatcag
     5041 cgctgcgctg atggctgcca cactgcccgc gtcggcctgg gctttcggtg tcgatgaagc
     5101 cgaggtgctg tcgcgctacg gcgctccgct gcacgcgcgc gtgcctatca gcctgggcga
     5161 cggcgatcgt atcgagggac ccgacgaact cagcgtgcgc gtgctgcgca ctgaaggttc
     5221 cgccgacacg ccctactcgt acagcgaaga ctccatcgag gcgtcagtgg ccggcagcgg
     5281 ccgcgacctg tgggtggaac tgcgcggcaa gcgcccgctg cgtgagccgc tgctgctgct
     5341 gcagctggaa gtgaggtccg gctacacgcg gctgacacgc gaattgccac tgatgctcga
     5401 cccgcccgaa accagcgcgc cttccgcgcc gctgcgcgcg cgcgccgttg cagaggcgcc
     5461 gctgcccgtc ctctctcctg cgccggcacc ggtcgcggct atcgcgctga ctccggcacc
     5521 gccggcggca gaatctgtgg ccgagccatc gcatgcgcac acatccgcgt cgcgccgcat
     5581 gcaccacccg cgcgcgcgcg cagctgctgc gcagggcgaa gaccgcggcc tgcacctggc
     5641 cggctggaat gcgcagtcct acacccaggc gacgcagccg ttgccgatgt tccagctcga
     5701 cgagcatttc gccagcctga gcgaactgtc gcccgcaccg gagccggccg atgccatggc
     5761 cgcggcggcc gcgcccgtgg cgccggcggc aacgcaagcc ggaggagcgg cgcccgcagc
     5821 tgcgcccgcg cccgcaatcg ccccggcgct gacgccgccc gcagataccc agccttcgtc
     5881 gcgcggcttg tggtggtggc tgctcgcggt cgggctcgtt gccgcgtacg ccgtgcgccg
     5941 gatgcgcctg gccccgcgcg cggttccacc gtcccgccgc gcgcgcggag tggccgggtc
     6001 ggacgcggca agagcggaag cgccggtgcc ggcagccgag gccgcgcact ccgccgtgga
     6061 ggacgccacg cagccgctgc gccgccgcat ggcggaactg gcgcgcaaca ttccttcgac
     6121 cgacaccgct ggcctgcgca agctgcaact ggtggaggcc tatctcgacc tgggcaagct
     6181 ggcgcaggcg caggccatgc tgtcggagct ggaagcggaa aaaattcgca cggagcgacc
     6241 cgccattacc ctggtcaagg gataggcggc catgaagtcc attggtgctg cgctgctgcg
     6301 cagcggggca gcgcgggcgg gcgcagcaat ggcgctcgcg gttacgagct gcgccgccgc
     6361 ggccgcgccc tcgccctcgc ccgcggacac gtacctgttg cccatcgttc ccgcgcatca
     6421 ctacacgccg cacctgaaac tggccggaga cttcgccagc ctcgccgccc gtcccgcgcc
     6481 ggtcgcggcc tcgccggcgg cgaataagtc tgctgtatcg agtccaacgg ccgacgaggt
     6541 gcgcgcattc aatggcacgc aagtcggcgt cggctccacc agctggccgg aagcggggcc
     6601 acacaagccg gttgcaacgg gcggaaccga cggccccgag gctcaaggaa actggcgctc
     6661 cggcagcctc ctgtccgtcc tgatcgtgcg cgcctcgtcc gcttcccgtc ccggactggt
     6721 cctgggcgcc gcgctcatcg tcggcctgct cgtcctgcgc ggtgccagca gcggcggcgg
     6781 cacacgccga aaactgcgcg atcgctcgcc gcgtgacgca atgacggccg cagaggaact
     6841 gccgtccgcc aaggtgcatg ccctggaacc ggccgaacgg ctgaacttcg acatgacgcg
     6901 gaaccaggcg cggggggaac cggccaccgc tgccggcgct gccgacacca tggagctgcg
     6961 caagcgcgca tcggcgcact gg
//
LOCUS       DASZZC010000575         6242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1141849,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000575 DASZZC010000000
VERSION     DASZZC010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141849"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHE37_12295"
     rRNA            <1..529
                     /locus_tag="VHE37_12295"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            650..763
                     /gene="rrf"
                     /locus_tag="VHE37_12300"
     rRNA            650..763
                     /gene="rrf"
                     /locus_tag="VHE37_12300"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(857..1192)
                     /locus_tag="VHE37_12305"
     CDS             complement(857..1192)
                     /locus_tag="VHE37_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36363.1"
                     /translation="MNTLLRFAAGVVFIISSIVSMPAQATASDSEFAQAEASCHAENQ
                     KVRTMETRGLSEEQMSGERAAYERACARLETLQGKLQPGGTAEIAAYEVQPAMPAGTQ
                     QDCSQKKPE"
     gene            complement(1277..2671)
                     /gene="rimO"
                     /locus_tag="VHE37_12310"
     CDS             complement(1277..2671)
                     /gene="rimO"
                     /locus_tag="VHE37_12310"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HVT36364.1"
                     /translation="MSSSRTVSRTRKARTPKVGFVSLGCPKALVDSERILTQLRAEGY
                     ETTPTYDAADVVIVNTCGFIDSAVTESLDAIGEAMAENGKVIVTGCLGAKSNVIREKF
                     PDVLSISGPQDYKTVMEAVHAQVPPKHDPFVDLLPPPRRLGAGADDVGVKLTPRHYAY
                     LKISEGCNHKCSFCIIPSMRGRLVSRPVADVLNEAERLVKAGVQELLVISQDTSAYGV
                     DLKYAKGSWRGREYAARMTELAGGLGELGAWVRLHYVYPYPHVDDVIPLMAEGKVLPY
                     LDVPFQHASARVLKLMKRPAAAEDTLERLAAWRKICPQLTVRSTFIVGFPGETEADFE
                     ELLGWLQQAQLDRVGCFKYSPVEGATANALPDAVPDEVKHQRYERFMQAQQAISRKKL
                     KAKVGRTIEVMVDELGGDADQPYLLGRSHADAPEIDGKVYLSCDRPLLPGQRVRAKVI
                     AADEYDLNAQVLED"
     gene            complement(2917..4305)
                     /gene="rmuC"
                     /locus_tag="VHE37_12315"
     CDS             complement(2917..4305)
                     /gene="rmuC"
                     /locus_tag="VHE37_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HVT36365.1"
                     /translation="MTPALILSVLLALALAAVLALWLRARPDGAQAKLVDAMRDEVSR
                     LRGELGELSRQQRQELGGVLGQSTQSLIAQQGELSSRLAQQVEQLRRALDERFTAFNT
                     DARAGREEGAATLHRHNSALTQQLAALTESNAQRMQELRGTLEARLKDIQSDNAAKLE
                     EMRRTVDEKLHATLELRLGESFKLVSERLEQVHKGLGEMQSLASGVGDLKRVLSNVKT
                     RGVLGEGQLGSLLEQVLTPEQYARNVATRPGSAERVEFAIRMPGGGEDGVPCWLPIDA
                     KFPREDYERLQDAYERADPAATEAAASALERRVKLEAQRIREKYLSPPHTTDFAILFV
                     PIEGLYAEVLRRPGLFELLQREQRIIVAGPVNLLAILNSLQMGFRTLAIEKRSSEVWQ
                     VLGAVKTEFAKYGEILDKVKKKLDEATSQIEQTGVRSRAISRRLREVQALPAADSERV
                     LGPADEADSEES"
     gene            complement(4379..4972)
                     /locus_tag="VHE37_12320"
     CDS             complement(4379..4972)
                     /locus_tag="VHE37_12320"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT36366.1"
                     /translation="MIRNTISIGLLAASLALGSNALAASPTDSVDAFHAALLDNMKHG
                     GACSARAQHLKPVIDKSFDVPFLAQRILRKHWSELSADQQKDFTAAFDDMIVSTYAAQ
                     FKSYSNESFDTQGTEDISAGNKVVHVLFKHGDSTKFDYVLHADSSGTLRIVNVIADGV
                     SDLAIRTAQYDKLYQAQGYAGLLAYIKDQTAKTKASC"
     gene            5044..>6242
                     /locus_tag="VHE37_12325"
     CDS             5044..>6242
                     /locus_tag="VHE37_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVT36367.1"
                     /translation="MKPENSAAADPVQRDRDSVIARLVARMQRHALAVVGVALALGAL
                     LAWYTAGHLSVDTNNQNMLSPGLAWRKAETELDRLFPGNAGSLVLVIDGTTPEVADDA
                     QRRLADAMKSEPALFSQVSAAEIEPYFRRNGLLYLSTGELQKLSDDLTQAQPFLGALN
                     EDPSLHGLFTLLDRAVTHADAADFDMSKALGDIAEGIDAARQEKFWRLSWQGLLGGPP
                     RPDATRRFITVDAVKDYSQLLPAEKPIDRVRALAQSLSLDAAHGVSVRLTGSTAMEHQ
                     ELQTAFGGIGLVLGVAMLMVMVLLYLALRSVRLVVCAVLTLIYGLLATASFAAATIGH
                     LNLISVAFSVLYVGLGLDYALYLTMQYRELIGHGLAHRVALPRAAREVGGFMLVCAAT
                     TSLGFFA"
BASE COUNT         1047 a         2011 c         1975 g         1209 t
ORIGIN      
        1 gaaagtaggt ctaagtgatc cggtggttct gtatggaagg gccatcgctc aacggataaa
       61 aggtactccg gggataacag gctgatactg cccaagagtc catatcgacg gcagtgtttg
      121 gcacctcgat gtcggctcat ctcatcctgg ggctgtagca ggtcccaagg gtccggctgt
      181 tcgccggtta aagaggtacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta
      241 tctgccgtgg gcgtttgaga tttgaggaga gctaacctta gtacgagagg accgggttgg
      301 acgcacctct ggtgttccag ttgtcacgcc agtggcatcg ctgggtagct atgtgcggac
      361 gggataaacg ctgaaagcat ctaagcgtga agcccactcc aagattagat ctcgcggacc
      421 ttcaaggtcc ctaaagagtc cacgtagact acgtggttga taggcgcgat gtggaagtgc
      481 agtaatgcat ggagctaacg cgtactaatt gctcgtgagg cttgaccata taacgctcaa
      541 gcattctatg ttccgttaat tactgaggca ttgagagttt tggtgttcag ctacagactg
      601 atttgaattc cgtgagaagc ggcaattgcg cttcttgcga gccgtctttc ctggcggcct
      661 tagcgagttg gaaccacctg ttcccatccc gaacacagaa gtgaaacgac tcagcgccga
      721 tgatagtgtg gcaagaccat gcgagagtag gacactgcca ggatcttaaa tgagcaaacc
      781 cgccccggaa ggggcgggtt ttttcatttg cggatttgat ttctacttcc gcgtagtggg
      841 aacaaggccg gcgactttac tccggcttct tctgcgaaca gtcctgctgt gtacccgccg
      901 gcatcgccgg ttgtacctca taggccgcga tctcggctgt accgcccggc tgcagcttgc
      961 cctgcagcgt ttccagcctg gcgcaggcgc gctcataggc cgcacgctcg ccgctcatct
     1021 gttcttccga cagcccacgt gtctccatcg tgcgtacctt ctgattttcc gcgtggcacg
     1081 aagcttccgc ctgggcgaac tcgctatcgc tggccgtggc ctgcgctggc atggacacga
     1141 tagacgagat gatgaagacg acaccggcgg cgaaacgcag caaggtattc atgggcactt
     1201 cctctgtgaa gtcggcggct caacgtccgc atttttcccc ggattggacc ctgcgccgca
     1261 gatcgtggtt cgcccttcag tcttccagca cttgggcgtt gaggtcgtac tcgtccgccg
     1321 cgatcacttt cgcgcgcact ctctgacccg gcagcagcgg gcggtcgcaa gacaggtaca
     1381 ccttgccgtc gatctccggc gcatccgcat ggctgcgccc gagcaggtag ggctggtcgg
     1441 catcgccgcc caattcgtcg accatgacct cgatcgtcct gccgaccttg gctttcagct
     1501 tcttgcggct gatggcctgc tgcgcctgca tgaagcgctc gtagcgctgg tgtttgacct
     1561 cgtcgggtac cgcatccggc agcgcgttgg cggtcgcgcc ttccactggc gaatacttga
     1621 agcaaccgac acggtcgagc tgcgcctgtt gcagccagcc gagcagctct tcgaaatccg
     1681 cttccgtctc gccgggaaag ccgacgatga acgtgctgcg cacggtgagc tgcgggcaga
     1741 tcttgcgcca ggccgccagc cgctccagcg tgtcctcggc cgcggcaggc cgcttcatca
     1801 gcttgagcac gcgcgcgctg gcgtgctgga acggcacgtc gaggtacggc agcaccttgc
     1861 cttccgccat cagcgggatg acgtcgtcga catgcgggta gggatacacg taatgcagcc
     1921 gcacccacgc gccgagttcg cccagcccgc ccgcgagttc ggtcatgcgc gctgcatact
     1981 cgcgtccgcg ccagctgccc ttcgcgtact tgaggtccac cccatatgcg ctggtgtcct
     2041 gcgagatcac cagcagttcc tgcacgccgg ccttcaccag gcgctcggcc tcgttcagca
     2101 catcggcgac gggccgcgac accaggcgcc cgcgcatcga cggaatgatg cagaagctgc
     2161 acttgtgatt gcagccctcg gagatcttca gataggcgta atgacgcggc gtgagcttga
     2221 cgcctacatc gtctgccccg gcgccgagtc gccggggggg cggcaacagg tccacgaacg
     2281 gatcatgttt aggcggcacc tgcgcatgca ccgcctccat caccgtcttg tagtcctgcg
     2341 gcccgctgat cgacagcacg tccgggaatt tttcgcggat gacgttgctt ttcgcgccga
     2401 ggcagccggt gacgatcacc ttgccgttct ccgccatcgc ctcgccgatc gcatcgagcg
     2461 actcggtcac cgcgctgtcg atgaagccgc aggtgttgac gatcaccacg tcggccgcgt
     2521 cgtaggtcgg cgtggtctcg tagccttccg cgcgcagctg ggtgaggatg cgttcggaat
     2581 cgaccagcgc cttggggcag ccgagcgaga cgaagcccac cttgggcgtg cgcgccttgc
     2641 gcgtgcgcga aactgtgcgg gaagaggaca ttgccgggaa acaggaagcg ggcgcctatt
     2701 ttaggtcaaa gccgcggtgc cgcccgcgca cgctcggcgc taggtggaaa aatcgacccg
     2761 tccgatttag gcgcgcttca tcgtcggttg agcaaaaaag cctgacaccg caccggccac
     2821 aacgacgtta ttaattatta aaatatttta aattaaaaca acaaaatata acagcgcaag
     2881 atgtcctgcg gtgaagggtt tttggtgtcc gcaagctcag ctttcttcgc tgtccgcttc
     2941 gtcagccggc cccagcacgc gctcgctgtc cgccgccggc agtgcctgca cctcgcgcag
     3001 gcgccgtgaa atcgcccggc tgcgcacgcc ggtctgctcg atctggctgg tcgcttcatc
     3061 gagcttcttc ttcaccttgt cgaggatctc gccgtacttg gcgaactcgg tcttcaccgc
     3121 gcccagcacc tgccacacct cgctcgagcg cttctcgatg gccagcgtgc ggaagcccat
     3181 ctgcaggctg ttgaggatgg ccagcaggtt caccggcccg gcgacgatga tgcgctgctc
     3241 gcgctgcagc agctcgaaca atccgggcct gcgcaacact tccgcgtata gcccttcgat
     3301 cggcacgaac aaaatggcga agtcggtggt gtgcggcggc gacaggtatt tctcgcggat
     3361 gcgttgcgct tccagtttta cgcgccgttc cagcgcggac gccgccgctt cggtcgcggc
     3421 cggatccgcg cgctcatagg cgtcctgcag ccgctcgtaa tcctcgcgcg gaaacttggc
     3481 gtcgatcggc agccagcacg gcacgccgtc ctcgccgccg ccgggcatgc ggatggcgaa
     3541 ctccacgcgt tccgcgctgc ccggccgcgt ggcgacgttg cgtgcgtact gctccggcgt
     3601 cagcacctgc tcgagcaggc tgccgagctg gccttcgccg agcacgccgc gcgtcttcac
     3661 gttggacagc acgcgcttga ggtcgccgac gcccgaggcc agcgactgca tctcgcccag
     3721 gcccttgtgc acctgctcca gccgctccga caccagcttg aacgattcgc ccaggcgcag
     3781 ctccagcgtg gcatgcagct tttcgtcgac cgtgcggcgc atctcctcca gcttcgcagc
     3841 attgtccgac tggatgtcct tgagccgcgc ctccagcgtg ccgcgcagtt cctgcatgcg
     3901 ctgcgcattg gattcggtga gcgccgccag ctgctgggtc agcgcgctgt tgtgccggtg
     3961 cagcgtggcc gcgccttcct cgcggccggc gcgcgcgtcg gtgttgaatg cggtgaagcg
     4021 ctcatccagt gcgcggcgca gctgctccac ctgctgcgcc aggcgcgagg acaactcacc
     4081 ctgctgcgcg atgagcgact gcgtggattg gccgagcacg ccgccgagtt cctggcgctg
     4141 ctggcgcgac agttcgccca gttcgccgcg caggcgcgaa acttcgtcgc gcatggcatc
     4201 gaccagcttg gcctgcgcgc catccggccg tgcgcgcagc cacagcgcca acacggcggc
     4261 gagcgccagc gcaagcagga cggaaaggat gagggctgga gtcatccgcc aagcttagca
     4321 agccctcggg aagccccgag aaatagcgcc gcgagcgcag cagcctttga gaacctccct
     4381 agcagctcgc cttggtcttg gcagtctggt ccttgatata ggccagcagg cccgcatagc
     4441 cctgcgcctg gtacagcttg tcgtactgcg cggtgcgaat ggccaggtcc gacacgccgt
     4501 cggcaatcac gttgacgatg cgcagcgtgc ccgacgaatc cgcgtgcagc acgtagtcaa
     4561 acttggtgct gtcgccgtgc ttgaacagca cgtgcaccac cttgttgccg gcggaaatgt
     4621 cctcggtgcc ctgcgtgtcg aagctctcgt tgctgtagct cttgaactgc gcggcatagg
     4681 tgctgacgat catgtcgtcg aacgcggcgg tgaaatcctt ctgctggtcg gccgacagct
     4741 cgctccagtg cttgcgcagg atgcgctgcg cgaggaaggg cacgtcgaag ctcttgtcga
     4801 tcaccggctt caggtgctgc gcgcgcgcgc tgcaggcgcc gccgtgcttc atgttgtcga
     4861 gcagtgcagc gtggaaggcg tcgacgctgt cggtcggcga ggcggccagc gcgttcgagc
     4921 ccagggccag tgaggcggca agcaaaccga tgctgatcgt gttgcggatc atggaatgga
     4981 aacctgtcgg aaaggtcgaa aaggtagcat acgcacctca caagaataga agcgcgcaac
     5041 tgaatgaagc ctgaaaattc cgcggccgcc gacccggtgc aacgcgaccg cgacagcgtc
     5101 atcgcgcggc tggtcgcgcg catgcagcgt catgcgttgg cggtggtcgg cgtggcgctc
     5161 gcgctcggcg cgctgctggc ctggtacacc gcggggcacc tgtcggtcga caccaacaac
     5221 cagaacatgc tgtcgccggg actggcctgg cgcaaggcag agaccgaact cgaccgcctg
     5281 ttccccggca acgccggcag cctggtgctg gtgatcgatg gcaccacgcc ggaggtggcc
     5341 gacgacgcac agcgccgtct ggccgatgcc atgaagtccg agcctgcgct gttcagccag
     5401 gtcagtgccg ccgagatcga gccgtacttc cgccgcaatg gcctgctcta tctctctacc
     5461 ggcgagctgc agaaactgtc cgacgacctg acccaggcgc aaccgtttct cggcgcgctg
     5521 aacgaagatc cgtcgctgca tggattgttc acgctgctcg accgcgcggt gacgcatgcg
     5581 gacgccgccg acttcgacat gagcaaggcc ctgggcgaca tcgccgaggg catcgatgcc
     5641 gcgcgccagg aaaaattctg gcgcctgtcg tggcagggcc tgctcggcgg cccgccgcgg
     5701 cccgatgcca cgcgccgctt catcacggtc gatgcggtga aggattactc gcagctgctg
     5761 ccggcggaaa aacccatcga ccgggtgcgc gcgctggcgc agtcgctgtc gctcgatgcc
     5821 gcgcatgggg tcagcgtgcg cctcaccggc tccaccgcga tggagcacca ggaactgcag
     5881 accgcgttcg gcggcatcgg cctggtgctc ggtgtggcca tgctgatggt gatggtgctg
     5941 ctgtacctgg cgctgcgctc ggtgcggctg gtggtgtgtg cggtgctcac gctgatttat
     6001 ggcctgcttg ccaccgcgtc gttcgccgcc gccaccatcg ggcacctgaa cctgatctcg
     6061 gtggcgttct cggtgctgta cgtcggcctg ggtctcgact acgcgctgta tctcaccatg
     6121 cagtaccgcg agctgatcgg ccatggcctg gcgcatcgcg tggcgctgcc gcgcgcggcg
     6181 cgcgaggtcg gcgggttcat gctggtgtgc gcggccacca cctcgctcgg attcttcgcc
     6241 tt
//
LOCUS       DASZZC010000576        10262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_813590,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000576 DASZZC010000000
VERSION     DASZZC010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813590"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VHE37_12330"
     CDS             complement(<1..1402)
                     /locus_tag="VHE37_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVT36368.1"
                     /translation="MIAAVEAHRAGLFSRPHWLPNIIAGVIVGVVALPLAMAFAIASG
                     AKPEQGIYTSIVAGLTVSAFGGSRIQIAGPTGAFVVILAGVTAKFGIDGLLLATLMAG
                     AMLVLLGIARLGAAMRYIPAPVISGFTAGIGVIIWVGQWPAFFGLPAPAGEHFYQKLY
                     HLLQALPQLHAPTTLLALLSLGLVIYGSRVPGLARVPGPMIALVAVTLINAIHPFAGV
                     ATIDSMFGGIPRGLPHFSFPAITLDRVVTLLPSAFTIAMLGAIESLLSAVVADGMAGT
                     RHDPNQELIGQGVANLLTPLFGGFAATGAIARTATNFRNGATSPLAGMVHSLTLVLIL
                     LLLSPLAGRIPLCTLAAILFVVAWNMSEAKHVLRMTRRAPQADVAILWVTFALTVMTD
                     LVVAVNIGVILAMLQFMRRMASSVEVLRLDEQQLRGELAHAAPLPKDLLVYSIEGPFF
                     FGAVESLEQALAHTHGD"
     gene            complement(1399..2244)
                     /locus_tag="VHE37_12335"
     CDS             complement(1399..2244)
                     /locus_tag="VHE37_12335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVT36369.1"
                     /translation="MSITDRLLPLLALLLPVLAQPADELMLKGDWTEGHVVLGHAPPG
                     TRAWFNGRALSVSDTGAFIFGLDRDARGSATLKLQLPGEKEPQLIRRDVATREYQVQR
                     IDGLPESKVNPPPSAMKQIERDAAILKAARKRDTAFNGFDQDMQWPLTGRITGSWGNQ
                     RILNGVPKSPHAGTDIAVPVGTPVAAPADGVVSVADPDMYYTGGTLMIDHGHGLQSML
                     IHLSRLKVKKGDVVRRGQIVAESGMTGRATGPHLHWGMFWFDARIDPQTLVPPMPVQE
                     KGATP"
     gene            complement(2231..3157)
                     /locus_tag="VHE37_12340"
     CDS             complement(2231..3157)
                     /locus_tag="VHE37_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HVT36370.1"
                     /translation="MDRSALTRYAWLSIAAAIATIGLKTLAWQLTGSMGLLSDALESL
                     VNLLSAIVAMIVLRIAAQPADAEHPYGHDKAEYFSSVLEGIAILLAAVSIAWAAVPRL
                     LHPQPVMEAPLGLAVNFVATLVNFGTSRVLLSAGRRHDSVTLQADAHHLMTDVWTSVG
                     VLAAVVLVVLTGWLVLDPLIALVVAAQIVWTGLRLVVTSVEGLMDSALPPPEQDALQE
                     VLNRYREQHGIAWHALRTRAAGSRRFVTVHVLVPGGWSVQRGHDLVEQMEAEMARRLK
                     RLTVVTHLEPLEDEVSWRDQELERPEETHEHH"
     gene            complement(3261..5198)
                     /gene="thrS"
                     /locus_tag="VHE37_12345"
     CDS             complement(3261..5198)
                     /gene="thrS"
                     /locus_tag="VHE37_12345"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HVT36371.1"
                     /translation="MAIQITFPDGNKKSFDGPVTGLAIAQGISPGLAKKAAVIKVDGE
                     LWDLTRPIDRDAAISIITRDKPEALEEIRHDAAHVLAQAVQELYPGTQITFGPATENG
                     FYYDFARSEPFTEADLGKIEQKMREIVKRDLPITREVWAHDDAVRHFEKAGEKFKAEW
                     IREGLKPDETLTIYKQGENWLDMCLGPHLPSTGKLGTAFKLTKVSGAYWRGDANNAQL
                     QRIYGLAFATDDDLKAYLHMMEEAEKRDHRKLGKQLDLFHQQEEAPGMVFWHPKGWAI
                     WQAVEQYVRKVYRKSGYQEVRAPQIIDVSLWKASGHWDNYKENMFFTESEKRTYAVKP
                     MNCPGHVQIFNAGLHSYRDLPIRYAEFGGCHRNEPSGALHGIMRVRAFTQDDGHIFCT
                     PEQVEGEVAAFHQQAMQVYADFGFTDISVKLALRPEKRLGTDDIWDRAEDAVRAALRK
                     VGVSWTELPGEGAFYGPKVEYHLKDSIGRPWQVGTMQIDFMMPGRLGATYIDEQSQRQ
                     HPVMLHRAIVGSMERFIGMLIEHHAGAMPMWLAPVQAVVAPIVSDADTYATEIVAALA
                     DAGLRAQPDLRNEKISYKIREHALQKVPVMVIVGRKEAEERTVTIRRRDGEQETVSLD
                     EGIKRLVGMTHIRASEVVDAV"
     gene            5314..7722
                     /locus_tag="VHE37_12350"
     CDS             5314..7722
                     /locus_tag="VHE37_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36372.1"
                     /translation="MTNPAGRGAVLLAAAVLQAGPLHAQEPAPSTIAVQEAAAPQAEG
                     GAAQLDEVTVTVQKRTQNLQKVPAAVGVIRSDTLEQTGSFDAAALEKYTPNIQIDNDP
                     QAPVIGVRGFSTETDNTGFEPSVGLTFDDLALGRPEFIADGFFDMDRIEVLRGTQGTL
                     FGKNTIAGVINFHTVEPDATRSAYLTATGGGHAQQRAEGGATLPLTDWLSSRVAGVWW
                     RKDGDIYNGTLNRYESSLDQKAGRVKLVADAGTGWKLGLSAQRSHTHVLYPPWQFDDA
                     SADAMAFARRYDASTEDDPLDGHTNENYPGFVDRNSALYRGIATLTLGNFLGASDVTA
                     TAVGGHAAFDFHAPFDVDVSAADLVNTLFDNADQQNTLELRLAGTSGSLLGLGHEVNW
                     VAGIFGLRARLVDEFDTLAGQDLAAFAASSAGLQALGAPRIALLNTLFASVPVTPGVP
                     LNDAVLRPYAQSTTSTALFGEMTWDLTDRLAAVIGARVGRDDKDAHFNVSVVGPGIIA
                     TIVGAKAFTADLARRETDFSPKLGLQYQWTRSLFSYVTASRGFKGGGFNATADTDSNL
                     QFEPERARSLEAGIKSEWFDRSLRINADLYRTDVSDLQVVDFVGTSFKVANAAEARLE
                     GIELETRWRPGWTWLSVDASVALSRAYYLSYPNAPPAPEQMGSGVPDPLGLMPAQTQD
                     LTGRTLQHAPRATATLSPAVTLPLLRNYAAMFGVDFSYRGQQYSSGDLDSHSFEAAYT
                     LLGARLVVGPQDLGWSFIVNGSNLANHRGAALVVNNPVYPHTYSAQETIPLRSWSASI
                     QARF"
     gene            complement(7719..9137)
                     /gene="gltX"
                     /locus_tag="VHE37_12355"
     CDS             complement(7719..9137)
                     /gene="gltX"
                     /locus_tag="VHE37_12355"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVT36373.1"
                     /translation="MSVVTRFAPSPTGFLHIGGARTALYSYLYARKHGGKFLLRIEDT
                     DRERSTQEAVDAIFEGMKWLGLNGDNDKPIYQTQRVGRYAEVIQQMLAAGTAYHCYAS
                     KAELDEMRAAQQARKEKPRYDGRWRPEPGKTLPPVPAGIPPVVRFKNPQEGAVVLDDL
                     IKGSITFASDELDDLIIARADGVPTYNFCVVVDDMDMGVTHVIRGDDHVNNTPRQINI
                     LKALGATPPRYAHVSMILGADGTKLSKRHGALGVMEYRALGFMPEAMLNYLVRLGWSH
                     GDQELFTREEMERLFDFDHVSAAPARFDMEKAYWVNHQYLKITDPRLIEPELRWHLQR
                     INVDPSGGPALTAVIEAQRERCKTLVEMAEKSKFFYAELDGYNEKDAAKHFTAEAGAL
                     LGELVMKLEALKEWTAPALHEAVNGLAEAKGLGLGKIAQPIRVAVAGMAISPPIDQTL
                     LLLGRERTLLRLAAACRKITGS"
     gene            9235..9705
                     /gene="hisI"
                     /locus_tag="VHE37_12360"
     CDS             9235..9705
                     /gene="hisI"
                     /locus_tag="VHE37_12360"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HVT36374.1"
                     /translation="MSDVEFKQRITIEQVEEGTDLAPKFDEHGLIACVTTAAKTGEVL
                     MLGYMNQEALKKTIATGEAHYWSRSRQVLWHKGATSGLVQKVVEMRIDDDQDAVWLRV
                     EIPGDASCHVGYRSCFYRSVPTGKDSGAPLHFEEDKKMFDPKKVYGDAPNPTQL"
     gene            complement(9804..>10262)
                     /locus_tag="VHE37_12365"
     CDS             complement(9804..>10262)
                     /locus_tag="VHE37_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36375.1"
                     /translation="LLNPGPGHWSTNNGGCVLRVAAGTHVLLLPADIEKPAEMRLLRE
                     HADRLHADVVVAPHHGSKTSSTDEFVRAVAPSLVLYSTGWRHHFHHPPPPVVQRYAAA
                     GAVGYNTAAGGTLEFSVTEAGVGEVREYRRESAKFWNADAEARFEPDRTR"
BASE COUNT         1699 a         3508 c         3362 g         1693 t
ORIGIN      
        1 gatcgccatg cgtgtgcgcc agcgcctgct ccagcgattc caccgcgccg aagaagaatg
       61 gcccttcgat ggaatacacc agcaggtcct tgggcaacgg cgccgcgtgc gccagctcgc
      121 cgcgcagctg ctgttcgtcg agccgcagca cttccaccga cgaggccatg cgccgcatga
      181 actgcagcat ggccaggatc acgccgatgt tcaccgccac caccaggtcg gtcatcacgg
      241 tgagtgcgaa ggtcacccac aggatggcca cgtcggcctg cggcgcgcgg cgcgtcatgc
      301 gcagcacgtg tttcgcctca ctcatgttcc aggcgaccac gaacaggatc gccgccagcg
      361 tgcacagggg aatgcgcccg gccaacggcg acagcagcag caggatcagc accagcgtga
      421 gcgagtgcac catgcccgcg agcgggctgg tggcaccgtt gcggaagttg gtggcggtgc
      481 gcgcgatggc gccggtggcg gcaaagccgc cgaacagcgg cgtcagcagg ttggccacgc
      541 cctgcccgat cagctcctgg ttggggtcgt gccgggtgcc ggccatgccg tcggcgacga
      601 ccgccgacag cagcgattcg atggcgccaa gcatggcgat ggtgaaggcc gacggcagca
      661 gcgtgaccac gcggtcgagc gtgatcgccg ggaaggaaaa atggggcagc ccgcgcggga
      721 tgccgccgaa catgctgtcg atggtggcaa cgccagcgaa cgggtggatg gcgttgatca
      781 gcgtgaccgc gaccagcgcg atcatcgggc ccggcacgcg cgccagcccc ggcacccgcg
      841 agccgtagat cactaagccc agcgacagca gtgcgagcag cgtggtgggc gcgtgcagtt
      901 gcggcagcgc ctgcagcagg tgatagagct tttgatagaa atgctcgccc gccggcgcgg
      961 gcagcccgaa gaacgccggc cactgcccca cccagatgat cacgccgatg ccggcggtga
     1021 agccgctgat caccggcgcc gggatgtagc gcatggccgc gcccaggcgc gcgatgccga
     1081 gcagcaccag catggcgccc gccatcagcg tggccagcaa caggccgtcg atgccgaact
     1141 tcgcagtgac gccggccagg atcaccacga acgcgccggt cggccccgcg atctggatgc
     1201 ggctgccgcc gaaggccgac accgtgagcc cggcgacgat cgaggtgtag atgccctgct
     1261 cgggcttggc gccggacgcg atggcgaaag ccatcgccag cggcagcgcg accacgccga
     1321 cgatcacgcc cgcgatgatg ttcggcagcc agtgcggccg cgagaacaat cctgcacggt
     1381 gcgcttcgac ggcggcgatc atggcgttgc gcccttctcc tgcaccggca tcggcggcac
     1441 cagggtctgc ggatcaatgc gcgcatcaaa ccagaacatg ccccagtgca ggtgcgggcc
     1501 ggtggcgcgg ccggtcatgc cggactcggc cacgatctgc ccgcgcctga cgacatcccc
     1561 cttcttcacc ttcagcctgc tcaggtggat cagcatcgac tgcaggccgt ggccgtggtc
     1621 gatcatcagc gtgccgccgg tgtagtacat gtcaggatcg gccaccgaaa ccacgccatc
     1681 ggccggcgcg gccaccggcg tgccgacggg cacggcgatg tcggtgcccg cgtgcggtga
     1741 tttcggcacg ccgttgagga tgcgctggtt gccccacgag ccggtaatgc ggccggtgag
     1801 cggccattgc atgtcctggt cgaacccgtt gaaggcggtg tcgcgcttgc gcgccgcctt
     1861 caggatcgcg gcatcgcgct cgatctgttt catcgccgac ggcggcggat tcaccttgga
     1921 ctcgggcaag ccgtcgatgc gctgcacctg gtactcgcgc gtggcgacat cacggcggat
     1981 cagctgcggc tccttctcgc cgggcaactg cagcttgagc gtggcgctgc ccctggcgtc
     2041 gcggtcaagg ccgaagatga acgctcccgt atcgctcacc gacagggcgc ggccgttgaa
     2101 ccaggcgcgc gtgccgggcg gcgcgtggcc cagcaccacg tggccctcgg tccagtcgcc
     2161 cttgagcatc aattcatcgg caggctgcgc cagcaccggc agcagcagcg cgagcagtgg
     2221 cagcagccgg tcagtgatgc tcatgggtct cctcgggccg ctccagttcc tggtcgcgcc
     2281 acgacacctc gtcttccagc ggttccaggt gcgtaaccac ggtaaggcgc ttgagccgcc
     2341 gcgccatctc cgcctccatc tgctccacca ggtcgtggcc gcgctgcacg ctccagccgc
     2401 cgggcaccag cacgtgcacg gtgacgaacc ggcgcgagcc cgcggcgcgc gtgcgcagcg
     2461 catgccaggc gatgccgtgc tgctcgcggt agcggttgag cacttcctgc agcgcgtcct
     2521 gctccggcgg cggcagtgcg ctgtccatca agccttcgac ggaggtgacc accaggcgca
     2581 ggccggtcca cacgatctgc gcggcgacca ccagcgcgat cagcggatcg agcaccagcc
     2641 agccggtgag caccaccagc accaccgcgg ccagcacgcc gaccgaggtc cacacgtcgg
     2701 tcatcaggtg gtgcgcgtcc gcctgcagcg tcaccgaatc gtgccggcgg ccggcggaca
     2761 gcagcacgcg cgaggtgccg aaattgacca gcgtggcgac gaagttcacc gccagcccca
     2821 gcggcgcctc catcaccggc tgcggatgca gcaggcgcgg aacggccgcc caggcgatcg
     2881 acaccgccgc cagcaggatg gcgatgcctt ccagcacgct ggagaaatac tccgccttgt
     2941 cgtggccgta cggatgctcg gcatcggccg gctgcgcggc gatgcgcagc acgatcatgg
     3001 cgacgatggc cgacagcagg ttgaccagcg actcgagcgc gtcggacagc aggcccatcg
     3061 agccggtcag ttgccaggct agcgtcttca ggccgatggt cgcgatcgcc gcggctatcg
     3121 acagccaggc gtagcgggtc agagcggatc gatccacgat tacagccgtc gtccccgcga
     3181 aggcggggac ccagttctga cgtttgcgtc tatctgcacg gaaaactgga ttcccgcctt
     3241 cgcgggaatg acgtgccttt tcataccgcg tcgaccactt cggaggcacg gatatgagtc
     3301 atgccaacga gcctcttgat cccctcgtcg agcgaaaccg tttcctgttc cccatcccgg
     3361 cgacgaatgg tgacggtgcg ttcctcggcc tccttgcgcc cgacgatgac catcaccggc
     3421 accttctgca acgcgtgctc gcggatcttg tagctgatct tctcgttgcg caggtccggc
     3481 tgcgcgcgca atccggcgtc ggccagcgct gccacgattt ccgtagcgta ggtgtcggca
     3541 tccgacacga tgggcgcgac caccgcctgc accggcgcca gccacatcgg catggcgccg
     3601 gcatggtgct cgatcagcat gccgatgaag cgctccatcg agccgacgat ggcccggtgc
     3661 agcatcaccg ggtgctggcg ctgcgactgt tcgtcgatgt aggtggcgcc cagacggccg
     3721 ggcatcatga agtcgatctg catggtgccg acctgccagg ggcggccgat ggaatccttg
     3781 aggtgatact cgaccttggg gccgtagaac gcgccctcgc ccggcagctc ggtccagctc
     3841 acgccgacct tgcgcagcgc cgcgcgcacc gcatcctcgg cgcggtccca gatgtcgtcc
     3901 gtgcccaggc gtttttccgg gcgcagcgcc agcttcaccg agatgtcggt aaagccgaag
     3961 tcggcataca cctgcatcgc ctgctggtgg aatgcggcca cttcgccctc gacctgttcc
     4021 ggcgtgcaga agatgtggcc gtcgtcctgc gtgaacgctc gcacgcgcat gatgccgtgc
     4081 agcgcgcctg aaggttcgtt gcggtgacag ccgccgaatt ccgcgtagcg gatcggcagg
     4141 tcgcggtacg aatgcaggcc ggcgttgaag atctgcacgt ggcccgggca gttcatcggc
     4201 ttcaccgcgt aggtccgctt ctcggactcg gtgaagaaca tgttctcctt gtagttgtcc
     4261 cagtggccgg acgccttcca cagcgacacg tcgatgatct gcggcgcgcg cacttcctgg
     4321 tagccgctct tgcggtaaac cttgcgcacg tactgctcaa ccgcctgcca gatggcccag
     4381 cccttgggat gccagaacac catgcccggc gcttcctcct gctggtggaa caggtcgagc
     4441 tgcttgccga gcttgcggtg atcgcgcttc tcagcctcct ccatcatgtg gagataggcc
     4501 ttgaggtcgt cgtcggtggc gaacgcgaga ccgtagatgc gctgcaactg ggcattgttg
     4561 gcatcgccgc gccaataagc tcccgatact ttcgtgagtt tgaatgctgt tcccagcttg
     4621 ccggtcgaag ggagatgcgg gcccaggcac atgtcgagcc agttctcgcc ctgcttgtag
     4681 atggtcagcg tttcgtcagg cttcaggcct tcgcggatcc actcggcctt gaacttctcg
     4741 ccggccttct cgaaatgtct caccgcgtcg tcgtgcgccc aaacctcgcg cgtgatcggc
     4801 aggtcgcgct tgacgatctc gcgcatcttc tgctcgatct tgccgagatc ggcctcggta
     4861 aagggctcgg agcgcgcgaa gtcgtagtag aagccattct cggtggccgg gccgaaagtg
     4921 atctgcgtgc ccggatacag ttcctgcacc gcctgcgcca gcacgtgcgc ggcgtcgtgg
     4981 cggatctctt ccagcgcctc gggcttgtcg cgggtgatga tgctgatcgc cgcatcgcgg
     5041 tcgatgggac gcgtcaggtc ccacaactcg ccgtcgacct tgatgacggc cgccttcttg
     5101 gccagccccg gcgaaatgcc ctgggcgatg gcgaggccgg tgacgggacc gtcgaaggat
     5161 ttcttgttgc cgtcgggaaa ggtgatctgg atggccatgg gcggggaatg gaatggaacg
     5221 gccgcaagcg ccggaccggc aaattttatc cgaacgcggc gccgcactcc ggtttgtcca
     5281 cggcagccgc tatgctggcg ccgggagaat tccatgacca atccagctgg ccgcggggca
     5341 gtcctgctcg ccgcggcggt gctccaggcc ggcccgctgc acgcgcagga gccggcgcct
     5401 tccaccattg cggtgcagga agccgctgcg ccgcaggccg agggtggcgc cgcgcagctc
     5461 gacgaagtga ccgtgacggt gcagaagcgc acgcagaacc tgcagaaggt gccggccgcg
     5521 gtcggcgtga tccgcagcga cacgctggag cagaccggca gcttcgacgc cgctgcactg
     5581 gagaagtaca cccccaacat ccagatcgac aacgatccgc aggcgccggt catcggcgta
     5641 cgcggcttct ccacggagac cgacaacacc ggcttcgagc cgagcgtcgg cctgaccttc
     5701 gacgacctcg cgctgggccg cccggagttc atcgccgacg gcttcttcga catggaccgc
     5761 atcgaggtgc tgcgcggcac ccagggcacc ttgttcggca agaacaccat tgccggcgtc
     5821 atcaacttcc atacggtgga acccgatgcg acgcgctccg cctacctgac ggcgaccggc
     5881 ggaggacacg cccagcagcg cgccgagggc ggtgccaccc tgccgctcac cgactggctg
     5941 tcgtcgcgcg tcgccggtgt ctggtggcgc aaggacggcg atatctacaa cggcacgctc
     6001 aaccgctacg agagttcgct ggaccagaag gcgggacgcg tgaaactggt tgccgacgcc
     6061 ggcacaggct ggaaactggg gctgtcggcg caacgttcgc acacgcacgt gctctacccg
     6121 ccatggcagt tcgacgacgc aagcgcggac gccatggcct tcgcccgccg ctacgacgct
     6181 tccaccgagg acgatccgct cgatgggcac accaacgaga actatcccgg tttcgtcgac
     6241 cgcaactccg cgctgtatcg cggcatcgcc acgctgaccc tgggcaactt cctcggcgcc
     6301 tcggacgtca ccgccaccgc ggtcggcggc cacgccgcgt tcgacttcca cgcgcccttc
     6361 gacgtggacg tgagcgccgc cgacctggtc aatacgctgt tcgacaacgc cgaccagcag
     6421 aacacgctcg aactgcgcct ggccggcacc agcggttcac tgctcgggct gggacacgag
     6481 gtcaactggg ttgccggcat cttcggcctg cgcgcgcggc tggtggatga gttcgatacg
     6541 ctggcgggcc aggacctggc agcgtttgcc gccagcagcg ccggactgca ggcgctcggc
     6601 gcaccgcgca tcgcgctgct caacaccctg tttgcgtcgg tgccagtcac gcccggcgtg
     6661 ccgctgaacg acgccgtgct gcggccctac gcacagtcca ccaccagcac cgccttgttc
     6721 ggcgaaatga cctgggacct gaccgacagg ctcgccgcgg tcatcggcgc gcgcgtcggc
     6781 cgcgacgaca aggacgcgca tttcaacgtc agcgtggtcg gccccggcat catcgccacc
     6841 atcgtcggcg ccaaggcctt caccgccgat ctcgcgcgcc gcgaaaccga tttctcgccc
     6901 aagctgggac tgcagtacca gtggacgcgc agcctgttca gctacgtcac cgccagccgc
     6961 ggcttcaagg gcggcggctt caacgccacg gcggacaccg acagcaacct gcagttcgag
     7021 ccggagcgcg cgcgcagcct cgaagccggc atcaagagcg agtggttcga ccgcagcctg
     7081 cgcatcaacg ccgacctgta ccgcaccgac gtgtcggacc tgcaggtggt cgatttcgtc
     7141 ggcacttcgt tcaaggtggc caatgccgcc gaggcacgcc tggagggcat cgagctggaa
     7201 acgcgctggc gcccgggctg gacctggctg tcggtggatg ccagcgtcgc gctcagccgc
     7261 gcctattacc tgagctaccc gaacgcgccg ccggcgccgg agcagatggg ttcaggcgta
     7321 cccgacccgc tcggcttgat gccggcacag acccaggacc tgaccggccg cacgctgcag
     7381 cacgcgccgc gcgccaccgc gacgctgtcg cccgccgtaa ccctgccgct gctgcgcaac
     7441 tacgccgcga tgttcggcgt ggacttcagc tatcgcggac agcagtactc ctcgggcgac
     7501 ctggactcgc atagtttcga ggcggcctac acgctgctcg gtgcgcgcct ggttgtcggc
     7561 ccgcaggatc tcggctggag cttcatcgtc aacggcagca acctggccaa ccatcggggc
     7621 gccgcgctgg tggtgaacaa cccggtgtat ccacacacct acagcgcgca ggaaaccatc
     7681 ccgctgcgca gctggtctgc ctcgatccag gcgcggttct agctgccggt gattttccgg
     7741 caggctgcgg cgagccgcag caacgtgcgc tcgcgtccga gcagcagcag cgtctggtcg
     7801 atcggcggtg aaatcgccat gccggcgacg gccacgcgga tcggctgcgc gatcttgccc
     7861 aggcccagcc ctttggcctc ggccagcccg ttcaccgctt cgtgcagggc cggcgcggtc
     7921 cattccttca gcgcctcgag tttcatcacc agttcgccga gcagggcacc ggcctcggcg
     7981 gtgaagtgct tggccgcatc cttctcgttg tagccgtcga gctcggcgta gaagaacttc
     8041 gacttctccg ccatttccac cagcgtcttg cagcgttcgc gctgcgcttc gatgaccgcc
     8101 gtcagagcgg gcccgccgga tggatcgacg ttgatgcgct gcaggtgcca gcgcagttca
     8161 ggctcgatga gcctggggtc ggtaatcttc aggtactggt ggttgaccca gtacgccttc
     8221 tccatgtcga agcgcgcggg cgccgccgac acgtgatcga aatcgaacag gcgctccatt
     8281 tcctcgcgcg taaacagctc ctggtcgccg tggctccagc ccagccgcac caggtaattc
     8341 agcatcgcct cgggcatgaa gccgagggcg cggtactcca tcacgcccag cgcgccatgg
     8401 cgcttggaca gcttggttcc gtccgcaccc aggatcatag acacgtgtgc atagcgcggc
     8461 ggcgtggcgc cgagcgcctt gaggatgttg atctgccgcg gcgtgttgtt gacgtggtcg
     8521 tcgccgcgga tgacgtgcgt gacgcccatg tccatgtcgt cgaccaccac gcagaagttg
     8581 taggtgggca cgccgtcggc gcgcgcgatg atcaggtcgt cgagctcgtc ggaggcaaac
     8641 gtgatgctgc ccttgatcag gtcatccagc accaccgccc cttcctgcgg atttttgaag
     8701 cgcacgaccg ggggaatccc ggccggcacc ggcggcagcg tcttgcccgg ctccggccgc
     8761 cagcggccgt cgtagcgcgg cttttccttg cgcgcctgct gcgcggcgcg catctcgtcc
     8821 agctccgcct tggaggcgta gcagtgatac gcggtgccgg cggcgagcat ctgctggatc
     8881 acctcggcat agcggccgac gcgctgcgtc tggtagatcg gcttgtcgtt gtctccgttc
     8941 aggcccagcc acttcatccc ttcgaaaatg gcgtccaccg cttcctgggt ggaacgctcg
     9001 cggtcggtgt cttcgatgcg cagcaggaat tttccgccat gcttgcgcgc atagagatac
     9061 gagtacagcg cggtgcgcgc gccgccgatg tgcaggaagc cggtcgggct gggcgcgaag
     9121 cgggtgacga cggacatggt gagagcggcc ggaagttgag tcggcaattt tatcagctag
     9181 gcagcgccaa ctagcctgag ggataatcgc gccccatttt tccgggccgc atccatgtcc
     9241 gacgtcgaat tcaagcagcg catcaccatc gaacaggtcg aggaaggcac cgatctcgcg
     9301 cccaagttcg atgagcacgg cctgatcgcc tgcgtcacca ccgcggcgaa gaccggcgag
     9361 gtgctgatgc tcggctacat gaaccaggag gcgctgaaga agaccatcgc caccggcgag
     9421 gcgcattact ggtcgcgctc gcgccaggtg ctgtggcaca agggcgccac ctcgggcctg
     9481 gtgcagaaag tggtcgagat gcgcatcgac gacgaccagg acgcggtgtg gctgcgcgtg
     9541 gagattcccg gcgacgcctc gtgccacgtc ggctaccgct cgtgcttcta ccgctcggtg
     9601 ccgaccggca aggacagcgg cgcgccgctg cacttcgagg aagacaagaa gatgttcgat
     9661 ccgaagaagg tctacggcga cgcgccgaat ccaacgcaac tctgagcggc ttgcggccgc
     9721 agaatcatct aaccgcactg ccctttaggt cgttattaat tatcgtatta tttaacattc
     9781 agtcgataat atataacatc aaatcaccgg gtgcggtcgg gctcaaaacg cgcttccgca
     9841 tcggcattcc agaattttgc cgactcgcgc cgatactcgc gcacctcgcc cacgcctgcc
     9901 tcggtcacgc tgaattcgag cgtgccgccg gccgcggtgt tgtagcccac tgcacccgcc
     9961 gccgcgtagc gctgcaccac cggcggaggc ggatggtgga aatggtggcg ccagcccgtg
    10021 ctgtacagca ccagcgacgg cgcgaccgcg cgcacgaatt cgtcggtcga tgaagtcttg
    10081 ctgccgtggt gcggcgccac caccacgtcg gcatgcagcc ggtccgcgtg ctcgcgcagc
    10141 aggcgcatct ccgctggctt ttcgatgtcc gccggcagca gcagcacgtg cgtgccggct
    10201 gccacgcgca gcacgcagcc gccgttgttg gtcgaccagt ggccggggcc gggattgagc
    10261 ag
//
LOCUS       DASZZC010000577         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_560716,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000577 DASZZC010000000
VERSION     DASZZC010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560716"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /gene="xerD"
                     /locus_tag="VHE37_12370"
     CDS             <1..756
                     /gene="xerD"
                     /locus_tag="VHE37_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HVT36376.1"
                     /translation="AGEADINDYLAARGSDRGEKFGARSQARLLSTLRAFYRFLIAER
                     QRDDDPTARIASPKIGRRLPRTLSEQDVENLLAAPDEEDALGLRDKAMLELMYASGLR
                     VSELVTLTLPRLDAERGVVQVIGKGGRERLVPMGELAMDWLRRYLRRARPELADGRAS
                     DTVFLTARGEGMTRHNFWHRLKAHARAAGIKAALSPHTLRHAFATHLLEHGADLRVVQ
                     ALLGHADLSTTQIYTHVSQARLAQLHAAHHPRA"
     gene            854..1627
                     /locus_tag="VHE37_12375"
     CDS             854..1627
                     /locus_tag="VHE37_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HVT36377.1"
                     /translation="MKHIAPVLLLAAPLLLSACAGEYDPTAPPKMAASGISKTVRQRL
                     ADAVGQPPDSISTSTVDGMLEAKWGTNFAYVTQDGRYAVFGDAVNLETQEEVTENQRR
                     GARVAALKELGDENTIEFAPAKTRYVVTVFTDVDCGYCRMLHRQVKEFNDEGIAFHYA
                     FYPRSGPDTDSFKKAQQVWCAADRKTALTNAKNGETPMGPANCVNPVMREWELGQKLG
                     LRGTPMLVLPNGEAISGYVPPQELLARLQSMNDKHAGKS"
     gene            complement(1759..>2229)
                     /locus_tag="VHE37_12380"
     CDS             complement(1759..>2229)
                     /locus_tag="VHE37_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVT36378.1"
                     /translation="DADPQVARRGIAVHRLLQWLTDGMNDERQLRQRLSAQLALTRAE
                     FGPWLGEARTLIAQPALAHLFAREKFRQAWNEVPLQDGDSLGVIDRLVDDGQTLWVID
                     YKTQRDADDAALLAHYAPQLRAYAAGAAKLYAGRKVQAGLLLTYSARWLPLPGE"
BASE COUNT          402 a          772 c          724 g          331 t
ORIGIN      
        1 gccggcgagg ccgacatcaa cgattacctc gccgcgcgcg gcagcgaccg cggcgaaaaa
       61 ttcggcgcgc gctcgcaggc gcggctgctg tccacgctgc gcgcgttcta ccgttttctc
      121 atcgccgaac ggcagcgcga cgacgacccc accgcgcgca tcgcttcgcc caagatcggc
      181 cggcgcctgc cgcgcacgct gtccgagcag gacgtggaaa acctgctggc cgcgccggac
      241 gaggaggacg cgctgggcct gcgcgacaaa gcgatgctgg aactgatgta cgccagcggc
      301 ctgcgcgtat cggaactggt gacgctgaca ttgccgcggc tcgacgccga gcgcggcgtg
      361 gtgcaggtga tcggcaaggg cggtcgcgaa cggctggtgc cgatgggcga actggcgatg
      421 gactggctgc gccgctacct caggcgcgcg cgcccggaac tggcggatgg ccgcgcgtcc
      481 gataccgtgt tcctcaccgc gcgcggcgag ggcatgacgc ggcacaactt ctggcaccgg
      541 ctgaaggcgc atgcgcgcgc cgccggcatc aaggccgcgc tgtcgccgca cacgctgcgc
      601 catgccttcg ccacgcacct gctggaacac ggcgccgacc tgcgcgtggt gcaggcgctg
      661 ctgggccatg ccgacctgtc gaccacgcag atctacaccc acgtcagcca ggcgcggctg
      721 gcccagctgc acgcggccca tcacccgcgc gcctgagcca ccggtgccag gatgggtttt
      781 caccacagcg gccgggaacg aaagcggaac tcatctgtca taatttctcc tcgcgttttt
      841 cgacctggcc accatgaagc acattgctcc cgtcctgctg cttgccgcac ccctgctcct
      901 gtctgcctgc gccggggaat acgatccgac cgcgccgccc aagatggccg cgtccggcat
      961 cagcaagacc gtgcgccagc gcctggccga cgccgtgggc cagccgcccg acagcatcag
     1021 cacctcgacc gtggacggca tgctcgaggc caagtggggc accaacttcg cctacgtgac
     1081 gcaggatggc cgctacgccg tgttcggcga cgcggtgaac ctggaaacgc aggaggaagt
     1141 gaccgagaac cagcgccgcg gcgcgcgcgt ggccgcgctc aaggaactgg gcgacgagaa
     1201 caccatcgag ttcgcgccgg cgaaaaccag gtacgtggtg accgtgttca ccgacgtcga
     1261 ctgcggctac tgccgcatgc tccatcgcca ggtgaaggag ttcaacgacg agggcatcgc
     1321 cttccactac gcgttctacc cgcgcagcgg gccggacacc gactcgttca agaaggcgca
     1381 gcaggtgtgg tgcgccgccg accgcaagac cgcgctgacc aacgccaaga acggcgagac
     1441 gccgatgggc ccggccaact gcgtcaaccc ggtgatgcgc gaatgggagc tgggccagaa
     1501 gctcggcctg cgcggcacgc cgatgctggt gctgcccaac ggcgaagcga tctccggcta
     1561 cgtaccgccg caggaactgc tggcgcggtt gcagtcgatg aacgacaagc acgccggcaa
     1621 gagctgattt gcggccctgg gagcgctaac cgcacgacca tttttgatgt tataaaaaat
     1681 cgtattaatt taaataagat taatatttta taacactcaa aatagcagtg cggttcagca
     1741 aagaaagcgc gtccgcagct attcgccggg cagcggcagc cagcgcgcgg agtacgtgag
     1801 cagcagtccc gcctgcacct tgcgccccgc gtagagtttg gccgcgccgg ccgcataggc
     1861 ccgcaactgc ggcgcgtaat gcgcgagcag cgccgcgtca tccgcatcgc gctgggtctt
     1921 gtagtcgatg acccacaggg tctgcccgtc atcgaccagg cggtcgatca cccccaggct
     1981 gtcgccgtcc tgcagcggca cctcgttcca cgcctgccgg aatttttcac gggcgaagag
     2041 atgcgcgagt gccggctgcg cgatcagcgt gcgcgcttcg cccagccacg ggccgaactc
     2101 cgccctggta agagccagct gcgccgacag gcgctggcgc aactggcgtt catcgttcat
     2161 cccatcggtc agccattgca gcaggcggtg caccgcaatg ccgcggcgcg ccacctgcgg
     2221 atcggcatc
//
LOCUS       DASZZC010000578         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_302355,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000578 DASZZC010000000
VERSION     DASZZC010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302355"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHE37_12385"
     CDS             <1..657
                     /locus_tag="VHE37_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36379.1"
                     /translation="ACPMRKPGKREQNKQANSAAILVAARQCFYKLGYDGVTIRDVIR
                     ATHLASGTFYNYFPDKESLFLALIEERMNALTARVSEVRRAARTLEAFLHGAYSTVFE
                     EICAHTEFYAMMFRNEPVIRGMFSDNVMGISMRSLKDDLADAVKRGLLPVMDVDYLTA
                     VFFGAGYELARMLTERKDKNPIDAALFATQLFLGGVQNAGSGTALIRRGSLKLGGAAR
                     "
     gene            complement(667..1368)
                     /locus_tag="VHE37_12390"
     CDS             complement(667..1368)
                     /locus_tag="VHE37_12390"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVT36380.1"
                     /translation="MDEVLKRRLIGALVVLCAVFLLTLVLPRPGDAPKPEPGTQRVVI
                     DLTQPVPRYAPAAAVTASSAPSAAPPEAPTTSTATADEPPEIETPAPEPQKMETPPPA
                     APAKPDAQPAATATAAAELKPTLRLEQKVSTPIPAPAPAAAAPRPVVSPTAKAPTKSA
                     ALQKAWYVQIGSYASLDNAHAALDPLKASNGLISPADTATGTLYRVRLGPYATKEQAK
                     AARERYAPKANLVED"
     gene            complement(1361..>2364)
                     /locus_tag="VHE37_12395"
     CDS             complement(1361..>2364)
                     /locus_tag="VHE37_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HVT36381.1"
                     /translation="YNERVGINGVDASDADFCRAFTAIEQARGDTPLTYFEFGTLAAL
                     WLFREAQVDVQVLEVGLGGRLDAVNLVDADAAIVTSIGLDHMDWLGPDRASIAREKMG
                     VARAGRPLVCAEIEPPDTIASCAAHAGARLLQLGADFDFVETGATWTWGTPEVQRRKL
                     PRPGLAGIAQLRNAAGVLSLVHALDDVLPVTDAAIRSALPALTLPGRCQRIGEVIVDV
                     AHNAQAAQVLADNLAAGGVDRVRLVLGMLGDKPVEDYCAVLAPQVERAYFATLPGPRG
                     LSSRVLAQRSGLRGALFDDVAAALAAARAESKGGEQVVVTGSFLTVAAAIEELRSHG"
BASE COUNT          367 a          810 c          801 g          386 t
ORIGIN      
        1 gcttgcccga tgcgcaagcc gggcaagcga gagcagaaca agcaggccaa cagcgccgcc
       61 atcctggtgg cggcgcgcca atgtttctac aagctgggct acgacggcgt caccatccgc
      121 gacgtgatcc gcgccaccca cctggccagc ggtaccttct acaactattt cccggacaag
      181 gagtcgctgt tcctggcgct gatcgaggag cgcatgaacg cgctcaccgc gcgtgtctcc
      241 gaagtgcgcc gcgccgcgcg cacgctggaa gcgttcctgc atggcgccta cagcacggtg
      301 ttcgaggaga tctgcgcgca caccgagttc tacgccatga tgttccgcaa cgagccggtg
      361 atccgcggca tgttctcgga caacgtcatg ggcatctcga tgcgttcgct gaaggacgac
      421 ctggccgacg cggtcaagcg tggactgctg ccggtgatgg atgtcgatta cctgacagcg
      481 gtgtttttcg gcgccggcta cgagctggcg cgcatgctga ccgagcgcaa ggacaagaac
      541 ccgatcgacg cggcgctgtt cgccacccag ctgttcctcg gcggcgtgca gaacgcgggt
      601 agcggcaccg cgctgatccg gcgcggctcg ctcaagctcg gcggcgcggc gcgctgagcg
      661 cgcgcctcaa tcctcgacca ggttggcctt gggcgcgtag cgttcgcggg ccgccttggc
      721 ctgctctttc gtcgcgtagg gtccgagtcg cacgcggtag agcgtgccgg tggcagtgtc
      781 ggcggggctg atcagcccat tggatgcctt caacggatca agcgcggcat gcgcattgtc
      841 caggctggca tagctgccga tctgcacgta ccaggctttc tgcagcgcag cgcttttcgt
      901 cggcgccttc gcagtcgggc tcactacagg tctaggcgca gctgcggccg gcgccggtgc
      961 tggaattggc gtgctgacct tctgctcgag cctgagcgtg ggtttgagtt cggcggcggc
     1021 cgtcgcagtt gcagcaggct gtgcatcggg ttttgcaggc gcggccggcg gcggggtttc
     1081 catcttttgc ggttcaggcg ccggcgtctc gatctccggc ggctcgtcgg cggtcgcggt
     1141 tgacgtggtt ggcgcttccg gaggcgcggc tgaaggtgcc gaagatgccg tgaccgcggc
     1201 cgccggcgcg tacctgggaa ccggctgcgt cagatcgatg accacgcgct gcgtgcccgg
     1261 ctccggcttt ggcgcatcgc ccggccgcgg cagcaccagc gtcagcagga ataccgcgca
     1321 cagcaccacc agcgcgccga tcagcctgcg cttgagcacc tcatccatgg ctgcgcagct
     1381 cctcgatggc tgcagccacc gtgagaaacg atccggtcac caccacctgc tctcctccct
     1441 tggattcggc gcgcgccgcg gccaatgcgg ccgccacgtc atcgaacaat gcgccgcgca
     1501 ggccagagcg ttgcgccagc accctggaag acaagccgcg gggccctggc aacgtggcga
     1561 aatacgcgcg ctccacctgc ggcgcgagca ccgcgcaata atcttccacc ggcttgtctc
     1621 cgagcatgcc cagcaccagg cggacgcggt ccacgccgcc ggccgcgaga ttatccgcca
     1681 gaacctgcgc agcctgcgcg ttgtgggcga catcgacgat cacttcgccg atgcgctggc
     1741 aacgccccgg cagggtcagc gccggcagcg cggagcggat cgccgcgtcg gtcaccggca
     1801 gcacatcatc cagcgcatgc accaggctga gtacgcccgc ggcattgcgc aattgcgcaa
     1861 tgccagcaag gccgggtcgc ggcagcttgc ggcgctgaac ttctggcgtg ccccaggtcc
     1921 aggtggcgcc ggtttcgacg aaatcgaaat cggcgcccag ctgcagcaag cgggcgccag
     1981 cgtgcgcggc gcacgaggcg atggtgtccg gcggttcgat ctcggcacag accagcggcc
     2041 ggccggcgcg cgccacgccc atcttctcgc gcgcgatgct ggcgcgatcg ggtccgagcc
     2101 agtccatgtg gtcgagcccg atgctggtga cgatggcggc atcggcgtcg accaggttca
     2161 ccgcatccag ccggccgccg aggcccacct ccagcacctg cacgtcgacc tgcgcctcgc
     2221 ggaacagcca cagcgcggcc agcgtgccga attcgaagta agtgagcggt gtgtcgccgc
     2281 gcgcctgctc gatggcggtg aacgcgcggc agaaatcggc gtcgctggca tcgacgccat
     2341 tgatccccac gcgctcgttg tagc
//
LOCUS       DASZZC010000579         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_421330,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000579 DASZZC010000000
VERSION     DASZZC010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421330"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VHE37_12400"
     CDS             <1..1146
                     /locus_tag="VHE37_12400"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HVT36382.1"
                     /translation="RIARALQAAVGAGAAASPDAPLAAALLIELRNLRGGVSQRAALL
                     GALLDELVSDPHLPATCSQALAGWTVPLFKAALADDSFFTNPAQPLRQLLSRETRAAV
                     TARTESDAAVQQAQARLAALPESFDVSVAFVQPALQQRKPLAHEQVLAFQQQQAAESA
                     ERRAELLAKVRRVVTHALEGRILGRTLPAALDELLRIGVGPLLARCLLRDGYGSQPWE
                     AELARVGLILDGAGNNDEFRRRGEALARLVMDLKQAGLSGEKAGALVQGLRDYYRQAD
                     EQAALMVPAIAAALDAPPPANDLLAQLLRPDQWFQVYDPQRSESCWMKVAAYAPAHDR
                     VTFVDFSGQKTLTMRASEFAGDLKTRRSGPVGASAEVQQALARLMAA"
     gene            1304..2458
                     /locus_tag="VHE37_12405"
     CDS             1304..2458
                     /locus_tag="VHE37_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HVT36383.1"
                     /translation="MSATALPVQWTDHKRYLWLLGVTAGAYPLTGYALFLLTGWKIFW
                     WYSPIWVYVLLPILDYFVGEDRDNPPEEMVPALAADSYYRYAVYASIPVQYISFVWCC
                     WMAVNGHLGWFEMTGLILSMGLVSGIAINTGHELGHQTNPLERWLAKISLAPGAYGHF
                     YVEHNRGHHVRVATPEDPATSRFGENFYEFFPRCVIGSIRSAWDIEKRRLEKQGHSVW
                     NWRNDNLQSWAMTAVVWGVMVAWLGPMVLPFIIAQAIYGGSLLEIVNYLEHYGLLRQK
                     KADGTYERCQPQHSWNSNHIVTNIFLYHLQRHSDHHANPTRSYQALRHFEDAPQLPSG
                     YSAMILLAYLPPLWFRVMDPKVVAHFKGDMSRANLKPSIREHVLARYQPA"
BASE COUNT          417 a          884 c          819 g          432 t
ORIGIN      
        1 cgcattgcgc gcgccctgca ggccgccgtc ggcgcgggcg ccgcggcatc gccagatgcg
       61 ccgctcgccg cggccctgct gatcgaactg cgcaacctcc gcggcggcgt cagccagcgc
      121 gccgcactgc tcggggcact gctcgacgaa ctggtgtcgg accctcacct gcccgccacc
      181 tgctcgcagg cgctggccgg ctggaccgtg ccgctgttca aggcagccct ggccgacgac
      241 agtttcttca ccaacccggc ccagccgttg cgtcaattgc tcagccgcga aacgcgcgct
      301 gccgtcaccg cacgcaccga aagcgatgcc gccgtgcagc aggcccaggc gcggctggcg
      361 gcgctgccgg aatcgttcga tgtcagcgta gcgttcgttc agcccgcgct gcagcagcgc
      421 aagccgctgg cgcacgaaca ggtgctcgcg ttccagcagc agcaggcggc tgaaagtgcg
      481 gagcgccgcg ccgagttgct ggccaaggtg cgccgcgtgg taacccacgc gctggagggt
      541 cgcatcctcg gccgcacgct gccggcagcg ctcgacgaac tgctgcgcat cggcgtcggc
      601 ccgctgctgg cgcgctgcct gctgcgcgac ggctacggca gccaaccctg ggaagcggaa
      661 ctggcgcgcg tcggcctgat cctcgacggc gccggcaaca acgacgaatt ccgccgccgc
      721 ggcgaagcgc tggcgcggct ggtaatggac ctgaagcagg ctggcctctc gggcgaaaag
      781 gcgggcgcgc tggtacaggg cctgcgcgac tactaccgcc aggctgatga gcaggccgcg
      841 ctgatggtgc cggcgattgc cgccgcgctg gatgcaccgc cgcctgccaa tgatctgctg
      901 gcgcagttgc tgcggccgga ccagtggttc caggtgtacg acccgcagcg cagcgaatcg
      961 tgctggatga aggtggccgc ctatgcgccg gcgcacgacc gcgtgacatt cgtggatttc
     1021 tccggtcaaa aaaccctgac gatgcgtgcc agcgaatttg ccggcgatct aaagacccgg
     1081 cgctccggcc ccgtcggcgc ctcggccgaa gtgcagcagg cactggcccg gctgatggcg
     1141 gcctgaaggc atttaccgaa tcgactcccc gcccagatct ccaacctctg ggcattactg
     1201 ggccggtctt ccgagaggat ttccggccct ttttttgttc aacgcacaat tgaacgaacg
     1261 gttgggccgc gctatggtgg cggcgccatc ggaggagaac cgcatgagcg caaccgcatt
     1321 gccggtccag tggaccgacc acaagcgtta tctctggctg ctcggcgtca cggcaggtgc
     1381 gtacccgctg acgggctatg cgctgttcct gctcaccggc tggaagatct tctggtggta
     1441 ttcgccgatc tgggtctacg tgttgctgcc gatcctcgac tacttcgtcg gcgaggaccg
     1501 cgacaatccg cccgaggaga tggtgccggc gctggctgcc gacagctact accgctatgc
     1561 ggtctatgcg tctatcccgg tgcagtacat cagcttcgtc tggtgctgct ggatggcagt
     1621 gaacggccac ctgggctggt tcgagatgac cggcctgatc ctgtcgatgg gcctggtctc
     1681 cggcatcgcc atcaacaccg gccatgaact gggacatcaa accaacccgc tcgaacgctg
     1741 gctggccaag atctcgctgg cgcccggcgc ctacgggcat ttctacgtgg agcacaaccg
     1801 cggtcaccac gtgcgggtgg ccacgccgga agacccggcg acctcgcgct tcggtgaaaa
     1861 cttctacgag ttcttcccgc gctgcgtgat cggcagcatc cgctcggcct gggacatcga
     1921 gaaacggcgg ctggagaagc agggccattc ggtatggaac tggcgcaatg acaacctgca
     1981 gtcctgggcg atgaccgccg tggtctgggg cgtaatggtc gcctggctcg gccccatggt
     2041 gctgcccttc atcatcgccc aggcgatcta tggcggatcg ctgctggaga tcgtcaacta
     2101 cctcgagcac tacggcctgc tgcggcagaa aaaagccgac ggcacctacg agcgctgcca
     2161 gccgcagcat tcgtggaaca gcaaccacat cgtcaccaac atcttcctgt atcacctgca
     2221 gcgccactcg gaccatcacg ccaacccgac gcgctcctac caggcgctgc ggcatttcga
     2281 agacgcgccg cagcttccca gcgggtattc cgcgatgatc ctgctcgcct acctgccgcc
     2341 gctgtggttc cgcgtgatgg acccgaaagt ggtggcgcac ttcaagggcg acatgagccg
     2401 cgcgaacctc aagccgtcga tccgcgaaca cgtgctggcc cgttaccagc cagcctgacg
     2461 ctccctggcc cggccgcacc attcaaggcc gcggcggttt tcgctactat tcgccccccg
     2521 ccggcatccc gccggcggtt tgctgtgggt gc
//
LOCUS       DASZZC010000580         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_370715,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000580 DASZZC010000000
VERSION     DASZZC010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370715"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VHE37_12410"
     CDS             complement(<1..220)
                     /locus_tag="VHE37_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36384.1"
                     /translation="MLGKLRQRLRGKDQVGVFKTTPDGRYLYADAALARIYRYPSPEH
                     MMIELRDIQKQLYVDPVRRLQFIFQMGRD"
     gene            complement(648..917)
                     /locus_tag="VHE37_12415"
     CDS             complement(648..917)
                     /locus_tag="VHE37_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36385.1"
                     /translation="MTKLALRGGFRRAAAALLLALGARVAWWSTQFDPLMLLGYAIIV
                     TAMMAMFVLFNEIAEARQRQQQVQELHEDAATQQSQPDRVIFPNR"
     gene            complement(914..2347)
                     /locus_tag="VHE37_12420"
     CDS             complement(914..2347)
                     /locus_tag="VHE37_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVT36386.1"
                     /translation="MVRATLTAAAAALLAACAAGPVYQAPATPTVAALPAHDGVYTAG
                     EPVAEFWRGFNDPLLAQLVEQALAGNTDLRAALARLDQARAITRQSRFDLLPTVTAEA
                     GYTQSRGSADQVPGVPRAQRDADLSHAALDAYWELDLFGRVRRNTEASRADTAALAAD
                     LRALQVSIAAEVARTYFELRGAQEQLQVARDNADNQRDTLKLTQARLDAGRGTEFDTQ
                     RGRAQLESTLARIPALEALVAIAAHRLAVLEGREPAALLAELQQAAPWPALPADVAVG
                     TPADLLRRRPDVQAAERRLAAATARIGVATADLFPRLTFGGSVGVSAGSLGDMFTRDG
                     ESYSFGPGVSWAFLDLGRVRARIAAADAGAEANLALYQGAVLHALEETENALVAYAHA
                     RSEGQHLQDAASASDAAAQLARTRFEGGIADFLQVLDAERSRLEAQERLSQSRERTAT
                     ALVAVYKSVAGGWPSHLPAAVARGDGP"
BASE COUNT          354 a          840 c          842 g          385 t
ORIGIN      
        1 cgtcgcgccc catctggaag atgaactgca gccgccgcac cgggtccacg tagagctgct
       61 tctggatgtc gcgcagctcg atcatcatgt gttccggcga cgggtagcgg tagatgcgcg
      121 ccagcgccgc atcggcgtag aggtagcgcc cgtccggcgt ggtcttgaac acgccgacct
      181 ggtccttgcc gcgcagccgc tgcctgagct tgcccaacat gtccgttctc ccggttgccg
      241 caagctcatc agcaagcgcc gtgccggaat ttttgaaacc cagcaaaaag gccggcgctg
      301 gagccggcct tgttgcgaca ctgccctggc cggcgtgccg atcgaggtgt ccatggacac
      361 gtagtagtcg ttgtcgttgg tgttggcgcc ggcgctgccg tcgacgtagc tgtacacctt
      421 gccggtctgc gcaatctgca ccagcccttc cggctgcgtg ccgcggtaga tgatggagga
      481 gagggtggaa cgtgcgcatg ccgggccgtc ctggccgtgc atagacgcgg gccttgccgt
      541 ccttggcgtg gcgtgctgtt ccaccctctc gaggtatttg accaacccca gccgcgaacg
      601 gctgaaaggc aaaccggcaa atttgcgcag gcaaaaaatg caaaaattta ccggttcgga
      661 aaaatgaccc ggtcaggctg tgactgctgc gtcgctgcat cctcgtgcaa ctcctgcacc
      721 tgctgctgcc gctggcgcgc ttcggcgatt tcgttgaaca gcacgaacat ggccatcatc
      781 gcggtgacga tgatcgcgta gcccagcagc atcagcggat cgaactgcgt gctccaccag
      841 gcgacgcgtg cgcccagcgc cagcagcaag gccgccgctg cccgccggaa tcctccgcgc
      901 aaggcgagct tggtcatggg ccatctccgc gggccacggc ggcaggcaga tgcgacggcc
      961 atccaccggc aaccgacttg tagaccgcaa ccagggccgt ggccgtgcgt tcgcggctct
     1021 ggctcaggcg ctcctgcgct tccagccgcg agcgctcggc gtcgagcacc tgcaggaaat
     1081 ccgcgatgcc gccttcaaag cgcgtgcgcg ccagctgcgc cgcggcgtcg ctggcggagg
     1141 cggcgtcctg caggtgctgg ccttcgctgc gcgcgtgtgc gtaggcgacc agcgcgtttt
     1201 cggtttcctc cagcgcgtgc agcaccgcgc cctggtacag cgccaagttc gcttcggcgc
     1261 cagcgtccgc cgcggcgatg cgcgcgcgca cgcggcccag gtccagaaag gcccagctga
     1321 cgccaggacc gaagctgtag ctttcgccat cgcgcgtaaa catgtcgccc aggctgccgg
     1381 cagaaacgcc gaccgacccg ccgaaggtca ggcgcggaaa caggtcggcg gtggctacgc
     1441 cgatgcgcgc ggtggctgcg gccaggcgcc gctccgccgc ctgcacgtcg ggccgccggc
     1501 gaagcaggtc agcgggcgtg cccacggcca cgtcggccgg caacgccggc catggcgcag
     1561 cctgctgcag ttcagcgagc agcgctgccg gctcgcggcc ctcgagcacc gccaggcgat
     1621 gggcggcgat tgccaccaac gcttccagtg ccggaatgcg cgccagcgtg gattccagct
     1681 gggcgcggcc gcgctgcgta tcgaactcgg tgccgcgccc ggcgtcgagc cgcgcctgcg
     1741 tcagcttgag cgtgtcgcgc tggttgtcgg cgttgtcgcg cgcaacctgc agctgttcct
     1801 gcgcgccgcg cagttcaaag taggtgcgcg ccacttcggc ggcgatgctg acctgcagcg
     1861 cacgcagatc ggcggcgagc gccgccgtgt cggcgcggct ggcctcggta ttgcggcgca
     1921 cgcgcccgaa caggtcgagt tcccagtagg catcgagcgc ggcgtggctg aggtcggcgt
     1981 cgcgctgtgc gcgtggcacg cctggcacct ggtcagcgct gccgcgcgac tgcgtgtagc
     2041 cggcttccgc cgtcaccgtc ggcagcaggt cgaaccgcga ttggcgcgtg atggcgcgcg
     2101 cctggtcgag ccgcgccagc gccgcgcgca gatcggtgtt gccggccagc gcctgctcga
     2161 ccagttgcgc cagcagcgga tcgttgaagc cgcgccagaa ctcggcgacc ggttcgccgg
     2221 ctgtgtaaac gccgtcgtgc gcgggcagcg cggcaaccgt gggcgtcgcc ggcgcctggt
     2281 acaccgggcc ggcggcgcaa gccgccagca gcgcggcggc cgccgcggtc agagtcgctc
     2341 ttaccatggc agtttctgcc cgaggtggaa gtagccgcca ttgggcgcat cgtcgccggc
     2401 cagcgccagc tgcacgctgg t
//
LOCUS       DASZZC010000581         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_51062,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000581 DASZZC010000000
VERSION     DASZZC010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51062"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VHE37_12425"
     CDS             complement(<1..542)
                     /locus_tag="VHE37_12425"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVT36387.1"
                     /translation="MSEQTSDYKYWAFISYSHADRAWGDWLIRKLETYRVPRRLVGRT
                     SRDGQVPARLFPVFRDRDELPTSADLGGVIDRALAQSRYQIVICSPRSAASRWVNEEV
                     KHFKSLGRGGRVLCLIVDGEPNASDQAGREAVECFAPALRFEVDAGRQLTDRRVEPIA
                     ADARAHADGKSGALLKLLAGL"
     gene            627..1541
                     /locus_tag="VHE37_12430"
     CDS             627..1541
                     /locus_tag="VHE37_12430"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVT36388.1"
                     /translation="MESLKTMLYQRDGRIARITLNRPARGNGITMDLPRELAACVERA
                     NLDREVHVIALAGNGSGFCGGYDLVDSAQEMKIGEPDPARPPGSVLDPLVQMKNHQPN
                     SAWDPMVDYAMMSRNVKGFMSLFHSEKPVVCKVHGFCVAGGTDMALCSDLLVIADDAK
                     IGYPPARVWGSPTTSLWAYRIGIEKAKRLLFTGDCLSGKEAQEWGLAIESAPADKLDE
                     RFEILLARIARMPVNQLVMMKLLLNQTMSAQGLHTTQILGTVFDGITRHTAEGYAFQQ
                     RAAEAGFRQAVRERDEPFGDFGLSTFKG"
     gene            complement(1521..>1916)
                     /locus_tag="VHE37_12435"
     CDS             complement(1521..>1916)
                     /locus_tag="VHE37_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVT36389.1"
                     /translation="VYEALAPALPHIEFPVTRRSRIVAGEPFFRQCEVPGEPNSETLI
                     ERIDVRGDLTLYRLQPHTGRTHQLRVHLAALGAPIVNDDFYPELRMAAEDDFSRPLQL
                     LARSIEFNDPLTGAPRHFASARRLTLEGG"
BASE COUNT          311 a          639 c          676 g          290 t
ORIGIN      
        1 agcccggcca gcagcttgag cagcgcgccg gacttgccgt cggcgtgcgc gcgcgcatcg
       61 gcggcgatcg gctccacgcg gcggtcggtg agctggcggc cggcgtcaac ctcgaagcgc
      121 agcgccggcg cgaagcattc caccgcctcg cgcccggcct ggtcgctggc attgggttcg
      181 ccgtcgacga tcaggcacag cacgcggccg ccgcggccga gcgacttgaa gtgcttcacc
      241 tcctcgttga cccagcgcga cgccgccgag cgcggcgagc agatcacgat ctggtagcgc
      301 gactgcgcca gcgcgcggtc gatcacgccg cccaggtcgg cggaggtcgg cagttcgtcg
      361 cggtcgcgga acaccgggaa caggcgcgcg ggcacctggc catcgcgcga ggtgcgtccc
      421 accagccgcc gcggcacgcg gtaggtctcc agtttcctga tcagccagtc gccccaggca
      481 cggtcggcgt ggctgtagct gatgaaggcc cagtacttgt agtcggacgt ctgctcggac
      541 atcgcgcggc ggtgtcgttt tcccgtgcgg ctagtgtagg tgcgcgcggc gctttacgct
      601 tgcgttgccc atcacggagc acacgcatgg aatcgctgaa gacgatgctg taccagcgcg
      661 acggccgcat cgcgcgcatc acgctcaacc gcccggcgcg cggcaacggc atcaccatgg
      721 acctgccgcg cgagctggcg gcgtgcgtgg agcgcgccaa cctcgaccgc gaagtgcacg
      781 tgatcgcgct cgccggcaac ggcagcggct tctgcggcgg ctatgacctg gtggacagcg
      841 cgcaggagat gaagatcggc gagccggacc cggcgcggcc gcccggctcg gtgctagatc
      901 cgctggtgca gatgaagaac caccagccca acagcgcgtg ggacccgatg gtcgactacg
      961 ccatgatgag ccgcaacgtg aagggcttca tgagcctgtt ccacagcgag aagccggtgg
     1021 tgtgcaaggt gcacggcttc tgcgtcgccg gcggcaccga catggcgctg tgctccgacc
     1081 tgctggtgat cgccgacgac gccaagatcg gctacccgcc ggcgcgtgtg tggggttcgc
     1141 cgacgacgtc gctctgggcg taccgcatcg gtatcgaaaa ggcgaagcgg ctgctgttca
     1201 ccggcgactg cctttccggc aaggaagcgc aggagtgggg gttggcgatc gaatcggcgc
     1261 cggccgacaa gctggacgaa cgcttcgaga tcctgctggc gcgcatcgcg cgcatgccgg
     1321 tcaatcaact cgtcatgatg aagttgctgc tcaaccagac catgagcgcg cagggcctgc
     1381 acaccacgca gatattgggc acggtgttcg acggcatcac gcgccacacg gcagagggct
     1441 acgccttcca gcagcgcgcg gccgaggccg gcttccgcca ggcggtgcgc gagcgcgacg
     1501 agccgttcgg cgacttcggc ctatccacct tcaagggtta gccggcgcgc actggcaaag
     1561 tgacgcggtg cgccggtcag cggatcattg aactcgatgc tgcgcgccag cagctgcagt
     1621 ggcctggaga aatcatcctc ggccgccatg cgcagctcgg gatagaaatc gtcgttgacg
     1681 atcggcgcgc ccagcgcagc caggtgcacg cgcagctggt gcgtgcggcc ggtgtgcggc
     1741 tgcaggcggt acagcgtgag gtcgccgcgc acgtcgatgc gctcgatcaa ggtttcgctg
     1801 ttcggctcgc ccggcacttc gcactggcgg aaaaacggct cgccggcgac gatccggctg
     1861 cggcgcgtga ccggaaattc gatgtgcggc agcgctggcg ccagcgcttc atacac
//
LOCUS       DASZZC010000582         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1014374,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000582 DASZZC010000000
VERSION     DASZZC010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014374"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2537)
                     /locus_tag="VHE37_12440"
     CDS             complement(<1..2537)
                     /locus_tag="VHE37_12440"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36390.1"
                     /translation="MIYNIIRPGAVSPKIAATISVALLALTGCGRSLPGATENVADAG
                     VAPAKLSQETQPADMSSSDGSGVFGAWTLDRYGLPAYDYTLDQVNDARAQRVELSGTT
                     SAWHQLGNDAIVANAYNDGYVQLWSSQRLYQWVNLYDASKQHYGGGYGYLRANGATTS
                     TLWLDRPAGVNATRRFGMGYVERDTPAGALDVREAVTAPFGDATALVHEIVISNSGGS
                     DASASWWEYWDANPALQITAQHVAVGLMAPAYDDAGKTVSIAQIADGTDTDPLSIFLT
                     AVDAPVSGFETDAKTFFGAGGRAAPDAVSADAPTNSVAAASLPAAGGSAMLALRAPVS
                     VPAHGSVTLRYVYGLAHAADIAGIVSGVAAQANTQAATASEWAARVPRADFGADKKWL
                     ARELAWDYYQTRSASLYEESCGHHIITQGGYYQYGLGEEEAFRDPLQHMLPMVYSAPE
                     LAREVLRYSLQEQPPDTGVIPYGMAALCTRFDFGASDDLDFWLLLSAVEYVLGTRDFA
                     FLDESVDYRGGVPVVPGVLSGTVWDHLKLAYHHQEDLVGRGIHGQYNIGPTGDWSDLS
                     PVFLQITESTLVTAQLAYIYPRLEELALAHGDLAFAQQLHDKAATLHDAVSGEWTGRG
                     WYSRGYSGTRQLGSGAIYGEPQPWAILAGIPDDAQAATLVNNIARYLTGIGAPAELHG
                     PALIGSSISPAKADPDISEFQIDPNGVGDGNAVYVGGAWYAIDGPLAWAQARLDGRVP
                     GAAERAWDELLRNTLKTHAQVFPQNWDGVINVDDACWSFYASDPSRCGIGIIIQLGGT
                     AGQITHQPAWGLYTTLKLSGIEPTGSGYDIRPHVPLAQWSLKLPLAG"
     gene            complement(2607..>3705)
                     /locus_tag="VHE37_12445"
     CDS             complement(2607..>3705)
                     /locus_tag="VHE37_12445"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVT36391.1"
                     /translation="NTVDPYLVSDRRTGRLFWAHATGPVRNEDTLPQDAGFYIAAASG
                     FQVYTSADNGYSWTTADYQTAPTGDWEQLMVGPPPAGSAQPVGYPDVLYLCANAPFEV
                     TGPGRLCYKSLDGGATFSVAGLVSPTASNPADICAPLQFRNGVVDSAGTLYIPATCQQ
                     ADYIIASHDEGASWSWSRFPDAPTSSSPTKVGGYLQMAVDDADNLYAMWNDPSDGLLY
                     LETSLDHGASWSAKQMVAAPGLKMVRRPAIAGGAAGQVGIAYYGSSDAAATLFTAWIT
                     QTRNGVDAQPLWYSAPLNDPAAPVFNDFALTGGRPRTDFVGGAFDAAGTTFWAGMVKQ
                     LPGEEDPEGAGIATVGLVGRLPFDDALGPLR"
BASE COUNT          547 a         1259 c         1285 g          614 t
ORIGIN      
        1 atgccggcca gcggcagctt cagcgaccac tgcgccagcg gcacgtgcgg gcggatgtcg
       61 taaccgctgc cggtcggctc gatgccagac agcttgagcg tggtgtacag gccccacgcc
      121 ggctggtgcg tgatctgccc ggcggtgccg ccgagctgga tgatgatgcc gatgccgcag
      181 cgcgacggat cgctggcgta gaacgaccag caggcgtcgt cgacgttgat cacgccgtcc
      241 cagttctgcg gaaacacctg cgcatgcgtc ttcagcgtgt tgcgcagcag ctcgtcccac
      301 gcgcgctcgg cggcgccggg cacgcggccg tcgaggcgcg cctgcgccca ggccagcggg
      361 ccgtcgatgg cgtaccacgc gccgccgaca tacaccgcgt tgccgtcgcc cacgccattc
      421 ggatcgatct ggaactcact gatgtcggga tcggccttgg ccggcgagat ggaactgccg
      481 atcagcgccg gcccgtgcag ctccgctggc gcgccgatgc cggtgaggta gcgcgcgatg
      541 ttgttcacca gcgtcgccgc ctgtgcgtca tcgggaatgc cggcaaggat cgcccagggc
      601 tgcggctcgc cgtagatggc gccgctgccc agctggcgcg tgccgctgta gccgcgcgag
      661 taccagccgc ggccggtcca ctcgccgctc accgcgtcgt gcagcgtggc ggccttgtcg
      721 tgcagctgct gcgcgaacgc gaggtcgccg tgcgccagcg ccagttcctc cagccgcggg
      781 tagatgtagg cgagctgcgc cgtgaccagg gtcgattcgg tgatctgcag gaacaccggc
      841 gagagatccg accagtcgcc ggtcgggccg atgttgtatt gcccgtggat gccgcggccg
      901 accaggtctt cctggtggtg ataggcgagc ttcaggtgat cccacaccgt gccgctgagc
      961 acgcccggaa ccacgggcac gccgccgcgg tagtcgacgc tctcgtcgag aaaggcgaaa
     1021 tcgcgcgtgc ccagcacgta ttctaccgcc gacagcagca gccagaaatc caggtcgtcg
     1081 ctcgcgccga aatcgaagcg cgtgcacagc gccgccatgc cgtacgggat tacgccggtg
     1141 tccggcggct gctcctgcag cgagtagcgc agcacctcgc gcgccagctc cggcgcgctg
     1201 tagaccatcg gcagcatgtg ctgcagcgga tcgcggaacg cttcttcctc gcccagcccg
     1261 tactggtaat agccgccttg cgtgatgatg tggtggccgc agctttcctc gtacagcgag
     1321 gccgagcgcg tctggtagta gtcccaggcc agttcgcgcg caagccattt tttgtcggcg
     1381 ccgaaatccg cgcgcggcac gcgcgctgcc cactcgcttg cggttgcggc ttgcgtgttt
     1441 gcctgcgcgg cgacgccgct gacgatgccc gcgatgtccg ccgcatgcgc caggccatag
     1501 acgtagcgca gcgtcacgct gccgtgcgcg ggcacgctca ccggcgcgcg cagcgcgagc
     1561 atggcgctgc cgccggccgc cggcaggctg gcggcggcga cggagttggt gggcgcatcc
     1621 gcgctcaccg cgtcgggcgc ggcgcggccg cctgcgccga agaacgtctt cgcgtcggtc
     1681 tcgaaaccgg acaccggcgc atcgaccgcg gtgaggaaga tcgacagcgg atcggtgtcg
     1741 gtgccgtcgg cgatctgcgc gatggagacg gtcttgcccg catcgtcgta cgcgggcgcc
     1801 atcaggccga cggccacatg ctgcgcagtg atctgcagcg cgggattggc gtcccagtat
     1861 tcccaccagc tggcgctggc atcgctgccg ccggaattgc tgatgacgat ttcgtgcacc
     1921 agcgcggtgg catcgccgaa cggcgcggtg accgcttcgc gcacgtcgag cgcgccggcc
     1981 ggcgtgtcgc gttccacgta gcccatgcca aagcggcgcg tggcgttgac gccggcggga
     2041 cggtcgagcc acagcgtgct ggtggtggcg ccgttcgcgc gcaggtagcc gtagccgccg
     2101 ccgtagtgct gcttgctggc gtcgtacaag ttgacccact ggtacaaccg ctgcgacgac
     2161 cacagctgca cgtagccgtc gttgtaggcg ttcgcgacga tcgcgtcgtt gcccagctgg
     2221 tgccacgcgc tggtggtgcc ggacaactcc acgcgctgcg cgcgcgcgtc gttcacctgg
     2281 tccagcgtgt agtcgtaggc cggcaggccg tagcggtcca gcgtccaggc gccgaacacg
     2341 ccggagccat cgctggagct catgtcggcc ggctgcgttt cctgcgacag cttcgccggc
     2401 gcgacgcccg catccgctac gttttccgta gcgcccggca gcgaacggcc acagccggtc
     2461 agcgccagca gggctacgga aatcgtagcg gcgatttttg ggctgaccgc acccggtctt
     2521 atgatgttat atatcattgt ctatttttac atttatacaa tattaaataa catcgcaaat
     2581 gccggtgcgg tggaagcatt tttcgctcag cgcaagggcc ccagcgcatc gtcgaacggc
     2641 agcctgccga ccaggcccac cgttgcaatg cccgcacctt ccggatcttc ctcgccgggc
     2701 agctgcttga ccatgccggc ccagaaggtg gtgccggcgg catcgaacgc gccgccgaca
     2761 aaatccgtgc gcggacgccc gccggtgagc gcgaagtcgt tgaacaccgg cgccgccgga
     2821 tcgttcagcg gcgcgctgta ccacagaggc tgtgcgtcga cgccgttgcg cgtctgcgtg
     2881 atccatgcgg tgaacagcgt ggccgccgca tcgctgctgc cgtagtaggc gatgcccacc
     2941 tgccctgctg cgccgccggc gatggccggg cgccgcacca tcttcaaccc gggcgccgcc
     3001 accatctgct tcgcgctcca gctggcgccg tggtcgagcg aggtttccag gtacagcagg
     3061 ccgtcgctgg gatcgttcca catcgcgtag agattgtcgg cgtcgtccac cgccatctgc
     3121 aggtagccgc ccaccttggt cggcgaactg ctggtcggcg catcgggaaa cctgctccag
     3181 ctccagctgg cgccctcgtc gtggctggcg atgatgtagt cggcctgctg gcaggtggcg
     3241 ggaatgtaga gggtgccggc gctgtcgacc acgccgttgc ggaactgcag cggtgcgcag
     3301 atatctgcgg gattgctggc cgtcggcgaa accaggcctg ctacggaaaa cgtagcaccg
     3361 ccgtcgagcg acttgtagca cagccggccc ggcccggtga cctcgaacgg cgcgttggcg
     3421 cacaggtaga gcacgtccgg gtaacccacc ggctgcgcac tgccggccgg cggcgggccg
     3481 accatcagct gctcccagtc gccggtgggc gcggtctggt aatcggcggt ggtccacgag
     3541 tagccgttgt cggccgaggt gtagacctgg aagcccgacg cggcggcgat gtagaagccg
     3601 gcgtcctgcg gcagcgtgtc ctcgttgcgc acgggcccgg tggcgtgcgc ccagaacagg
     3661 cggccggtgc ggcggtcgga caccaggtac ggatcgaccg tgttc
//
LOCUS       DASZZC010000583         6286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_108825,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000583 DASZZC010000000
VERSION     DASZZC010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108825"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VHE37_12450"
     CDS             <1..719
                     /locus_tag="VHE37_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVT36392.1"
                     /translation="VFFVIPWYGRTLVGTTEQEIRDPAEARPTAEEISYLIAGVRSGL
                     PGLKWSEGDVIGAFSGVRTLQAENEADLSAVTREFAITEPKPGLILPIGGKYTTSRGD
                     AADIVDRVFRVLKKKAPPSRTHALPLPGAPAGDFESWRADAAAQLAADGVDAEAAQWL
                     ALRHGTRVERIHQLLAEHPSWRDRIHHEVPFIQAEAVLAVRDEMALSADDIARRRMPL
                     SLLVRDNGWKKVIAQLTGCS"
     gene            848..3106
                     /locus_tag="VHE37_12455"
     CDS             848..3106
                     /locus_tag="VHE37_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36393.1"
                     /translation="MRPVKKIVLVLCLGLLSACSQQNNGDAGLTQSKAGVFSLFDPTA
                     ASPTIPFPFDGLFAGFTTPTLNIPNAGNVPFVNDANKQDGFSTTASIFTDFIGFVDMS
                     SLFAAPPALSGLIVIDSSTHAPLIPGVDYTVQPSNAIDSSGHPISAYRTRLLIEPLKP
                     LKPSTTYVVAITSALKSADGVPATPSDIFNVAASGTAVSAQTAPILATLNDTQKATLE
                     ALRAQLIHPTVAALVAAGIPQQAIVLAWPFTTESVGKTLTAVNTNAAAASLAVLSTGL
                     STLQASQGRAPDGGTVFLGTITVPYYLSAPSVADPTAALTGFWQADTAHPDNAAVFLG
                     QVPCPAFDPSSPGHVAAFSPGASTTTCFPRPLKQSDQKIPVLVTVPNAASGQTMPTAG
                     WPVVIFQHGITGDRSQMLGIAPTLAKAGFVTVAIDLPLHGITDTTSPLYRNQAFATAA
                     PGFTTGERTFDLDLSNNGTGAPGPDGSIDGSGTYFINLSSLITSRDNLRQAVADLIVL
                     TKSVPGTVFLAGGAPTADKIDGSRIYFVGHSLGGIVGSTLLGVNSNIRAATLAMPGGG
                     IAKLLDASKAFGPRISAGLAAKGVIEGTDDYETFMRFAQTLTDSGDPINFAAAARGGH
                     AIHMIEVVGSSTTPPDLVVPNHALANDGTGAYDKVTLTGFLSGTDPLYQQMGLATAST
                     DPLSVPIAAQPLLTGASLGVVVQFAQGNHGSILDPSGGATNAAVTCEMQRETAAFLLS
                     DGKALPLGGACP"
     gene            3137..4891
                     /locus_tag="VHE37_12460"
     CDS             3137..4891
                     /locus_tag="VHE37_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HVT36394.1"
                     /translation="MNKQLYQLTGLAAASLFAGHADALSFNVGDVSISSDNRLSIGGA
                     VRMQQRDSEIIGIANGGTADSTNHDDGNLAFGRGTPVFMGSKLTSDLTVSYGKFGGYV
                     RGSYIFDPVADKHDYFNAANYGAGKSAPVSEFNERMRAVQGHLGQNGRLLDAYIYGSF
                     DLADHNVGFKLGRQVINWGESTLIVNGINSFVAADQNKLRVPGFELAEVFTPMPAAWF
                     SAGLPAGFSMEAFYQLQWQQTYPDAVGSFFSTQDYAGYGATRANIGFGRAPENTPNTT
                     VPRAPDVDPKNGGQFGVKVEHVLPIFNDMDLALYAVQYHSRLPLLSGVSRQTFGAPSE
                     LASYFEEFPENIRLYGASFNTTLPWDLALQGEYSFKQGQPLQIDDVELLLAGLGVPSQ
                     IDPVLGDTLGGKYIRGWRRYNVSQVNLGLTRIFGPQDWLGTDQVLMLLEAADMYVHNL
                     PPSSVLRFDAPATDTPGDCTLEAAACTGGVTQNTAPYASRNSWGYRLLTRLTYNNVLP
                     GLALEPTFLFSHDVAGTSPTPIADFIAGSMAARAGIGAHFLSDWSGEVAYTNFFGGGR
                     RNLMNDRDYAEFNIKYSF"
     gene            complement(4913..5608)
                     /locus_tag="VHE37_12465"
     CDS             complement(4913..5608)
                     /locus_tag="VHE37_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HVT36395.1"
                     /translation="MKPALRRAIRRWFWIGVIGFVVLVALSNRWVINSADAYMYTDAS
                     LLPETEVGLVLGTSPHLADGKPSPSFFGRIRAAVDLYQQGKVRHLIVSGANPDSTYNE
                     PRRMLQELLKAGIPQKDITMDFAGFRTLDSIQRAQAVWGLSQFTIITQRSHCFRAVFL
                     GKKLGLNVVGYVARMGEQGDDFGPRNPIREIFARPLAVLDLVILKTQPKFLGAPEHLE
                     LRPAEPPPTAPGS"
     gene            complement(5605..6009)
                     /locus_tag="VHE37_12470"
     CDS             complement(5605..6009)
                     /locus_tag="VHE37_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVT36396.1"
                     /translation="MTGITALLLFAAWTLVLMFTYVNYRVMLTLTGKKPANSWTRGVP
                     NDDPPFVVRAQHAHYNCVENLPVFAAIVLAAAVLGKSAVVDAVACAYLGLRIAQSAVH
                     LISTSATMVFIRAVLFTGQVLLSFWMIWGLLH"
     gene            complement(6063..>6286)
                     /locus_tag="VHE37_12475"
     CDS             complement(6063..>6286)
                     /locus_tag="VHE37_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36397.1"
                     /translation="TAGIVFAGASGTADTWFRLAPADRAIALAAGAQAAPELGPDWVR
                     LPLFRPNWPKLDLVHWARKAYAHARGQAG"
BASE COUNT         1067 a         2247 c         2012 g          960 t
ORIGIN      
        1 gcgtgttctt cgtcatcccc tggtacggcc gcacgctggt cggcaccacc gagcaggaaa
       61 tccgcgatcc ggccgaagcg cggccgacgg cggaggaaat ctcctatctc atcgccggcg
      121 tgcgcagcgg cctgcccgga ctgaagtgga gcgagggcga cgtcatcggc gccttctccg
      181 gcgtgcgcac gctgcaggcg gaaaacgagg cggacctgtc ggcggtgacg cgcgagttcg
      241 ccatcaccga acccaagccg gggctgatcc tgcccatcgg cggcaagtac accacctcgc
      301 gcggcgacgc cgccgacatc gttgaccgcg tgttccgcgt gctgaagaaa aaggcgccgc
      361 cctcgcgcac gcacgcgctg ccgctgccgg gcgcgccggc gggcgacttc gagagctggc
      421 gcgccgatgc cgccgcgcag ctcgccgccg atggggtcga cgcggaagcc gcgcagtggc
      481 tggcgctgcg tcacggcacg cgggtggagc gcatccacca gctgctggcg gagcatccat
      541 cgtggcgcga ccgcatccac cacgaggtgc cgttcatcca ggccgaggcg gtgctggcgg
      601 tgcgcgacga gatggcgctg tcggccgacg acatcgcgcg gcggcgcatg ccgctgtcgc
      661 tgctggtgcg cgataacggc tggaaaaaag taatcgccca actaaccggc tgttcgtaag
      721 ccgcctcaac tgacagccga ttcagcccgc tgctgaccag cggcggaaaa tctgctgatt
      781 gtcggcgtca tcttttttta cgcatgtaac agactgccgc ccttcaattt cctgacgggc
      841 gaaccgcatg cgacctgtaa aaaaaatcgt gctcgtgctg tgcctcggac tgctgtccgc
      901 ctgcagccag cagaacaacg gcgacgccgg cctcacgcaa agcaaggccg gcgttttttc
      961 tttgttcgat cccaccgcgg ccagcccgac catcccgttt ccgttcgatg gcctgttcgc
     1021 cggcttcacc acgccgaccc tgaacattcc caacgccggc aacgtgccgt tcgtgaatga
     1081 cgccaacaag caggacggct tctccaccac ggcctcgatc ttcaccgact tcatcggctt
     1141 cgtcgacatg agctcgctgt tcgcggcgcc gccggcgctg tcgggcctga tcgtgatcga
     1201 cagctccacg cacgcgccgc tgattcccgg cgtcgactac acggtgcagc cgtcgaacgc
     1261 catcgacagc agcggccacc cgatctcggc ataccgcacg cgcctgctga tcgagccgct
     1321 caagccgctg aagccctcga ccacttatgt cgtcgccatc accagcgcgc tgaagagcgc
     1381 cgacggcgtg ccggccacgc cgagcgacat cttcaacgtg gcggcgtcgg gcacggcggt
     1441 gtcggcgcag accgcgccga tcctggccac gctgaatgac acgcagaagg ccacgctgga
     1501 agcgctgcgt gcgcagctga tccacccaac cgtcgccgcg ctggtcgccg ccggcatccc
     1561 gcagcaggcg atcgtgctgg cctggccgtt caccaccgag tcggtcggca agacgctgac
     1621 cgcggtcaac accaacgccg ccgctgcatc gctcgccgtg ctgtccaccg ggctcagcac
     1681 gctgcaggcc tcgcagggcc gcgcgccgga tggcggcacc gtgttcctgg gcaccatcac
     1741 cgttccgtat tacctgtcgg cgcccagcgt ggccgaccca accgcggcgc tcaccggttt
     1801 ctggcaggcg gacacggcgc atcccgacaa cgccgcggtg tttctcggcc aggtgccgtg
     1861 cccggcattc gatccttcca gcccggggca tgtcgccgcg ttttcaccgg gcgccagcac
     1921 caccacctgc ttcccgcggc cgctgaagca gagcgaccag aagataccgg tgctggtcac
     1981 cgtgcccaac gcagccagcg gccagaccat gcccacagcc ggctggccag tggtgatctt
     2041 ccagcacggc atcaccggcg accgctcgca gatgctcggc atcgcgccga cactggcgaa
     2101 ggccggcttc gtcaccgtgg ccatcgacct gccgctgcac ggcatcaccg acaccaccag
     2161 cccgctgtac cgcaaccagg cgttcgccac cgcggcgccg ggcttcacca ccggcgaacg
     2221 caccttcgat ctcgacctgt cgaacaacgg caccggcgca cccgggcctg acggcagcat
     2281 cgacggcagc ggcacctact tcatcaacct gtccagcctg atcaccagcc gcgacaacct
     2341 gcgccaggcg gtggccgacc tgatcgtgct gaccaagtcg gtgccgggca cggtgttcct
     2401 ggccggcggc gcgccgaccg cggacaagat cgacggcagc aggatctact tcgtcggcca
     2461 ctcgctcggc ggcatcgtcg gcagcacact gctgggcgtt aacagcaaca tccgcgcggc
     2521 cacgctggcc atgcccggcg gcggcatcgc caagctgctc gacgcgtcga aggccttcgg
     2581 tccgcgcatc agcgcgggcc tcgcggccaa aggcgtgatc gaaggcaccg acgactacga
     2641 aaccttcatg cgcttcgcgc agacgctgac cgactccggc gacccgatca acttcgccgc
     2701 cgcggcgcgt ggcggtcacg ccatccacat gatcgaggtc gtcggcagca gcaccacgcc
     2761 gcccgacctg gtggtaccga accacgcgct ggccaacgat ggcaccggcg cgtatgacaa
     2821 ggtcacgctg accggcttcc tctcgggcac cgacccgctg taccagcaga tgggcctggc
     2881 cacggccagc accgatccgc tgtcggtgcc gatcgccgcg cagccgctgc tcaccggcgc
     2941 cagcctgggc gtggtggtgc agtttgcgca agggaaccac ggctcaatcc tcgacccgtc
     3001 cggcggcgcc accaatgccg cggtcacctg cgagatgcag cgcgagaccg ccgccttcct
     3061 gctcagcgat ggcaaggcac tgccgctcgg cggcgcctgc ccgtaaaccg ccgtctccac
     3121 tttccggatc acaccaatga acaaacagct ctaccagctc accggtctcg cggcggccag
     3181 cctgttcgcg ggccatgccg acgctctcag cttcaacgtc ggcgatgtca gcatcagctc
     3241 ggacaaccgc ctgtcaatcg gcggcgccgt gcgcatgcag cagcgcgatt ccgagatcat
     3301 cggcatcgcc aacggcggca ccgccgacag caccaaccac gacgacggca acctggcctt
     3361 cggccgcggc acgccggtgt tcatgggcag caagctcacc tccgatctca ccgtcagcta
     3421 cggcaagttc ggcggctacg tgcgcggcag ctacatcttc gacccggtgg cggacaagca
     3481 cgactatttc aacgccgcca actacggcgc gggcaagtcg gcgccggtgt ctgaattcaa
     3541 cgaacgcatg cgcgcggtgc aggggcacct gggccagaac ggccgcctgc tcgatgcgta
     3601 catctacggc agcttcgacc tggccgatca caacgtcggc ttcaagctcg gccgccaggt
     3661 gatcaactgg ggcgagtcga cgctgatcgt caacggcatc aacagcttcg tcgccgccga
     3721 ccagaacaag ctgcgtgtgc cgggcttcga actggccgag gtgttcacgc cgatgccggc
     3781 ggcgtggttc tccgccgggc tgccggcggg cttcagcatg gaggcgttct accagctgca
     3841 atggcagcag acctatcccg atgccgtcgg cagcttcttc agcacgcagg actacgccgg
     3901 ctacggcgcc acgcgcgcca acatcggctt cggccgcgcg ccggagaaca cgcccaacac
     3961 cacggtgccg cgcgcgccgg acgtcgatcc gaaaaacggc ggccagttcg gcgtgaaagt
     4021 cgagcacgtg ctgccgatct tcaatgacat ggacctggcg ctgtacgcgg tgcagtacca
     4081 cagccgcctg ccgctgctgt cgggcgtgtc gcggcaaacc tttggtgcgc catccgaact
     4141 ggccagctac ttcgaggaat tcccggagaa catccgcctg tacggcgcct cgttcaacac
     4201 cacgctgccc tgggacctgg cgctgcaggg cgagtacagc ttcaagcagg gccagccgct
     4261 gcagatcgac gacgtcgaac tgctgctcgc cggcctgggc gtgccgagcc agatcgaccc
     4321 ggtgctgggc gatacgctcg gcggcaagta catccgcggc tggcgccgct acaacgtgtc
     4381 gcaggtcaac ctcggcctga cgcgcatctt cggaccgcag gactggctcg gcaccgacca
     4441 ggtgctgatg ctgctggaag cggccgacat gtacgtgcac aacctgccgc cgtcctcggt
     4501 gctgcgcttc gacgcgccgg ccaccgacac cccgggcgac tgcacgctgg aagccgcggc
     4561 ctgcaccggc ggtgtgacgc agaacaccgc gccgtacgcc tcgcgcaatt cctggggcta
     4621 tcgcctgctc acgcgcctga cctacaacaa cgtgctgccg ggcctggcgc tggagccgac
     4681 cttcctgttc tcgcacgatg tggccggcac ttcacccacg ccgatcgcgg atttcatcgc
     4741 cggcagcatg gcggcgcgcg cgggcatcgg cgcgcatttc ctgtcggact ggagcggcga
     4801 agtggcctac accaacttct ttggcggcgg ccggcgcaac ctgatgaacg accgcgacta
     4861 cgccgagttc aacatcaagt actcgttcta gccgtcacgc caccggctgc gctcagctgc
     4921 cgggcgcggt cggcggcggt tcggccgggc gcagttcgag gtgctcgggc gcgccgagga
     4981 acttgggctg cgttttcagg atgaccaggt cgagcaccgc cagcgggcgg gcgaagatct
     5041 cgcggatggg attgcgcgga ccgaagtcat cgccctgctc gcccatgcgc gccacgtagc
     5101 cgaccacgtt gaggccgagc ttcttgccca ggaacacggc gcggaagcag tgcgagcgct
     5161 gggtgatgat ggtgaactgc gacagtcccc acaccgcctg cgcgcgctgg atcgaatcca
     5221 gcgtgcggaa gccggcgaaa tccatggtga tgtctttctg cgggatgccg gccttgagca
     5281 gttcctgcag catgcgccgc ggctcgttgt aggtggagtc aggattggcg ccggacacga
     5341 tcaggtggcg caccttgccc tgctggtaca ggtccaccgc cgcgcggatg cggccgaaga
     5401 acgatggact cggcttgccg tcggccaggt gcgggctggt gcccagcacc agcccgacct
     5461 cggtttccgg cagcagcgac gcatcggtat acatgtaggc gtcggcgctg ttgatcaccc
     5521 agcggttgct cagcgcgacc agcacgacga agccgatgac gccgatccag aaccagcgcc
     5581 ggatcgcgcg ccgcaaagca ggtttcaatg caatagaccc cagatcatcc agaacgacag
     5641 cagcacctgg ccggtgaaca gcaccgcgcg gatgaacacc atggtggcgc tggtgctgat
     5701 caggtgcacg gccgattgcg cgatgcgcag gcccaggtag gcgcaggcca ccgcgtcgac
     5761 caccgcgctc ttgcccagca ccgccgccgc cagcacgatg gccgcgaaca ccggcaggtt
     5821 ctccacgcag ttgtaatgcg catgctgcgc gcgcaccacg aacggcgggt catcgttggg
     5881 cactccgcgg gtccaggagt tcgccggctt cttgccggtg agcgtgagca tgacgcggta
     5941 gttcacgtag gtgaacatca gcaccagggt ccaggcggcg aacagcagca aagcggtgat
     6001 accggtcatg cgagttctcc ctttgttgtg gtgacgcgca ttctagcggc agcggccctg
     6061 ctttagcctg cttggcctcg tgcatgcgca taggccttgc gcgcccagtg gaccaggtcg
     6121 agcttcggcc agttgggccg gaacagcggc aggcgcaccc agtccggtcc gagctcgggc
     6181 gctgcctgcg cgccggcggc cagcgcgatc gcgcgatcgg cgggcgccag ccggaaccag
     6241 gtgtcggcgg tgcccgacgc gccggcgaac acgatgccgg cggtga
//
LOCUS       DASZZC010000584         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1142420,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000584 DASZZC010000000
VERSION     DASZZC010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142420"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VHE37_12480"
     CDS             complement(<1..892)
                     /locus_tag="VHE37_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT36398.1"
                     /translation="MSLLTRVVRRASRIGERGLLWVSHAVERQFNTDNFIAANQTPFE
                     VIHQEGLLSVRRYPPLPADQCVVAETHRIPVLLVPPLAADPLNFDLLPQRSLVRFLLA
                     RGFRVYLADFGSPDEDHAHLALADYTTRMLPAAIAAVRRDSGERKISLLGYCMGGLFC
                     LIYAGWSHDRELASIVTIASPIDADQAGIAGKLWSAMRVPARLVRRYTGFRIHNLDPG
                     LLNVPGWVSSIVFKLTNPMGTITGYIDLLLNLWDRDYVTEYQTMSTWFNRMHDYPGGI
                     VQDFVVRVGLDNALARGRIGL"
     gene            complement(889..2775)
                     /gene="speA"
                     /locus_tag="VHE37_12485"
     CDS             complement(889..2775)
                     /gene="speA"
                     /locus_tag="VHE37_12485"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HVT36399.1"
                     /translation="MNDMTAWTAADARELYNMPLWGEGYFDVNEAGHVVVRPHRNAGA
                     IDLYELAQRLPREGLKLPVLVRFNDILHDRVDALTGAFAHVIDELGYQGRYTAVYPIK
                     VNQQRSVVEEIVRAGGARVGLEAGSKPELAAVLGLAPDGCIVVCNGYKDRDYIRRALI
                     GQKLGHRVYIVVEKPSELPVVIEEAEALGVSPLIGLRVRLASIGAGKWQNTGGEKSKF
                     GLSCSQVLEALASLRRAGRLDCVRMVHFHLGSQVANVQDIQRGMREAARFYAELRTLG
                     APVDVVDVGGGLGVDYEGTRSRGSCSMNYSQREYARNIVRTLAEVCAASGAPQPDIIS
                     ESGRALTAHHAVLITSVIDQESLPASQPQPPAKDDPAILHELHGLIGAVERRGPLECL
                     HDAASALAEAQAQYTHGVLTLPQRAYAEKTYVGVCRAVLPLLDLSVRAHREAADELRE
                     KLADKYFCNFSVFQSVPDAWAIDQVFPIMPLHRLNERPALRASLCDLTCDSDGRIDQY
                     VDRGGLEPTLPLHALRSGEDYLLAFFMVGAYQEILGDMHNLFGDTNAVNVALDEKGGY
                     RLEGAEAGDRTDELLRYVHIEPEELARAYRRKFEAAQMPAPQRAQLLAELESGLSGYT
                     YLGG"
     gene            2828..>3538
                     /gene="speE"
                     /locus_tag="VHE37_12490"
     CDS             2828..>3538
                     /gene="speE"
                     /locus_tag="VHE37_12490"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HVT36400.1"
                     /translation="MALDKSWFTEPMEKTGTAFSLKAKRLHSEKTPYQKIEIWQTETF
                     GYLMTIDGCTMVSTRDNFLYHEMMSHPALNSHPDPKTVVIVGGGDCGTLKEVLKHKGV
                     QSATQVEIDERVTRLAEKYFPELCEANGDKRATLYFGDGIQWMKDAAPGSIDLIIIDS
                     TDPVGPAEGLFGKRFYKDCIKALAKDGMLVQQSESPLLHAELIREMHAAMRDAGFAQT
                     HLLHFPQPIYPSGWWSASI"
BASE COUNT          602 a         1162 c         1205 g          569 t
ORIGIN      
        1 ccaggccgat gcggccgcgc gccagcgcgt tgtccagccc gacgcgcacc acgaagtcct
       61 gcacgatgcc accgggataa tcgtgcatgc ggttgaacca ggtgctcatg gtctggtact
      121 cggtgacgta gtcgcgatcc cacaggttga gcagcaggtc gatgtagccg gtgatggtgc
      181 ccatcgggtt ggtcagcttg aacacgatgg acgagaccca gccgggcacg ttgagcaggc
      241 cgggatcgag gttgtggatg cggaagccgg tgtagcggcg caccagccgc gccggcacgc
      301 gcatggcgct ccacagcttg ccggcgatgc cggcctggtc ggcgtcgatc gggctggcaa
      361 tggtgacgat gctggccagt tcgcggtcat gcgaccagcc ggcgtagatc aggcagaaca
      421 gcccgcccat gcagtagccg agcagggaga tcttcctttc gccggaatcg cggcgcaccg
      481 cggcgatggc ggccggcagc atgcgcgtgg tgtagtcggc cagcgcgaga tgcgcgtggt
      541 cctcgtcggg cgagccgaag tcggccaggt aaacgcggaa gccgcgcgcg agcaggaagc
      601 gcaccagcga gcgctgcggc agcaggtcga agttgagcgg gtcggctgcc agcggcggca
      661 ccagcagcac cgggatgcgg tgtgtttcag caacgacaca ctggtccgcc ggcagcggcg
      721 ggtacctgcg cacgctgagc aggccttcct ggtggatcac ctcgaacggc gtctggttgg
      781 cggcgatgaa attgtcggtg ttgaactggc gctccaccgc gtggctcacc cacagcaggc
      841 cacgctcgcc gatgcgcgag gccctgcgca cgacgcgcgt gagcaggctc atccgcccag
      901 gtaggtgtag ccggacaggc ctgactcaag ctcagccagc agttgcgcgc gctgcggcgc
      961 gggcatctga gccgcctcga acttgcgccg gtaggcgcgc gccagttcct ccggctcgat
     1021 atgcacgtag cgcagcagct cgtcggtgcg gtcgccggcc tcggcgcctt ccaggcggta
     1081 gccgcccttt tcatcgagcg ccacgttcac cgcgttggtg tcgccgaaca ggttgtgcat
     1141 gtcgcccaaa atttcttgat aagcgccgac catgaagaag gcgagcagat aatcctcgcc
     1201 ggatctgagc gcgtgcagcg gcaatgtcgg ctccagtccg ccgcggtcga cgtactggtc
     1261 gatgcggcca tcggagtcgc aggtgaggtc gcacaggctg gcgcgcagcg cggggcgctc
     1321 gttaagccgg tgcagcggca tgatcgggaa cacctggtcg atggcccagg cgtccggcac
     1381 cgactggaac accgagaagt tgcagaaata cttgtcggcc agcttttcgc gcagctcgtc
     1441 ggcggcttcg cggtgcgcgc gcacggaaag atccagcagc ggcagcactg cgcgacacac
     1501 gccgacgtag gtcttctccg cataggcgcg ctgcggcagc gtgagcacgc cgtgcgtgta
     1561 ctgcgcctgc gcctcggcca gcgcgctggc ggcgtcatgc aggcattcga gcgggccgcg
     1621 ccgctccact gcgccgatca gcccgtgcag ctcgtgcagg atggccggat cgtccttggc
     1681 cggcggctgc ggctgcgatg cgggcagcga ttcctggtcg atcaccgagg tgatcagcac
     1741 tgcgtggtgt gcggtgagcg cgcggccgga ttcgctgatg atgtccggct gcggcgcgcc
     1801 gctggcggcg cacacctcgg ccagcgtgcg cacgatgttg cgcgcgtatt cgcgctgcga
     1861 gtagttcatc gagcaggaac cacgcgaacg cgtgccttcg tagtccacgc ccaggccgcc
     1921 gccgacatcg accacgtcga ccggcgcgcc gagcgtgcgc agctccgcgt agaagcgcgc
     1981 agcctcgcgc atgcctcgct ggatgtcctg cacgttggcc acctgcgagc cgagatggaa
     2041 gtgcaccatg cgcacgcagt cgagccggcc ggcgcggcgc agcgaggcca gcgcttccag
     2101 cacctgcgag caggacaggc cgaactttga tttctcgccg ccggtgttct gccacttgcc
     2161 ggcgccgatc gaggccaggc gcacgcgcag gccgatcagc ggcgacacgc cgagcgcctc
     2221 ggcttcctcg atcaccacgg gcagttccga cggcttctcc accacgatgt acacgcggtg
     2281 gccgagcttc tgcccgatta gcgcgcggcg gatgtagtcg cggtccttgt agccgttgca
     2341 caccacaatg caaccgtcgg gcgcgaggcc gagcaccgct gccagttcgg gcttgctgcc
     2401 ggcttccagc cccacgcgcg cgccgccggc gcgcacgatc tcctccacca cgctgcgctg
     2461 ctggttgacc ttgatcgggt acaccgcggt gtagcggccc tggtagccga gctcgtcgat
     2521 cacgtgcgcg aaggcgccgg tgagcgcgtc gacgcggtcg tgcaggatgt cgttgaagcg
     2581 caccagcacc ggcagcttca gtccttcgcg cggcaggcgc tgcgccagtt cgtacaggtc
     2641 gatggcaccg gcgttgcggt gcgggcgcac caccacgtgg ccggcctcgt tcacgtcgaa
     2701 gtagccctcg ccccacagcg gcatgttgta cagctcgcgc gcatcggcgg cggtccaggc
     2761 ggtcatgtca ttcattcgca gtcacgcacc tttatactcc ggcgccaatt ttacggaaga
     2821 aaccctcatg gcactcgata aatcctggtt caccgagcca atggaaaaga ccggcacggc
     2881 gttttcgctc aaggccaaaa ggctccacag cgagaagacg ccgtaccaga agatcgagat
     2941 ctggcagacc gagaccttcg gttacctgat gaccatcgac ggctgcacca tggtcagcac
     3001 gcgcgacaac ttcctctacc acgagatgat gtcgcacccg gcgctgaact cgcaccccga
     3061 tccgaaaacc gtggtgattg tcggcggcgg tgattgcgga acgttgaagg aagtgctcaa
     3121 gcacaagggc gtgcagtcgg ccacgcaggt ggagatcgac gagcgcgtca cgcgcctggc
     3181 ggaaaaatat ttccccgagc tgtgcgaggc caacggcgac aagcgcgcca cgctgtattt
     3241 cggcgacggc atccagtgga tgaaggacgc ggcgccgggc tcaatcgacc tgatcatcat
     3301 cgactccacc gacccggtcg gcccggccga agggctgttc ggcaagcgtt tctacaagga
     3361 ctgcatcaag gcgctggcga aagacggcat gctggtgcag cagtcggagt cgccgctgct
     3421 gcatgcggag ttgatccgcg agatgcacgc ggcgatgcgt gacgccggct tcgcgcagac
     3481 gcacctgctg cacttcccgc agccgatcta cccgagcggc tggtggagtg catccatc
//
LOCUS       DASZZC010000585         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_371135,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000585 DASZZC010000000
VERSION     DASZZC010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371135"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VHE37_12495"
     CDS             <1..321
                     /locus_tag="VHE37_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187547.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVT36401.1"
                     /translation="IKSGGEWISSIDLENIAVAHPAVAEAAVIGIAHPKWDERPLLII
                     VKKPGKDVTREELLKFYEGKIAKWWMPDDVAFVSEIPHTATGKILKTRLREDFRQHRL
                     PTAG"
     gene            complement(324..>1777)
                     /locus_tag="VHE37_12500"
     CDS             complement(324..>1777)
                     /locus_tag="VHE37_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36402.1"
                     /translation="LLWATAAVIFAVALFLRFVVLARAPLTDDESAYLFAAHSLLRGH
                     LWENSPPLKEFYDRVFLINDGRMHSQYFLGWPALLAPAVAAGMGDLLNPLLCALTVFP
                     LARLLERYCRAPAVLAGLAWYAASMTSLVTAATLLAYTSALFFLTWYLHLVQRLTHDA
                     DAAPRDAALAALCGSMAFFIRPSAVVMIGAPATLLMLVHLLRSRPAAGRTLLAPFAGI
                     AAAFATLFLYLNQLIYGNALSNGYDKLSRGYSDYLLASVTDIGRMLETATAGLQRLTA
                     DISGWPLPLLALLAVLLAGRRLWVFSAALALEFCTCMVMSDLGIDTFGPLHLLEATTV
                     FILLLSLGMQRLTDALAPPLHSDGARVLLSTALGLVIAAWLTYFPLRVLNVGIIAANV
                     NGPLHLAARLPEPSVIFARRPFVYQNSIAPLRHYVFFMPISHPDMNDRVIWANNLGER
                     NAELLRTLPGRKAYTLEWDDAGRPQLQPYAAAD"
BASE COUNT          344 a          557 c          638 g          238 t
ORIGIN      
        1 atcaagtccg gcggcgagtg gatcagctcg atcgacctgg agaacatcgc cgtggcgcac
       61 ccggcggtgg ccgaggcggc ggtgatcggc atcgcccatc ccaagtggga cgagcggccg
      121 ctgctgatca tcgtgaagaa acccggcaag gacgtgacgc gcgaggaact gctcaagttc
      181 tacgaaggca agatcgccaa gtggtggatg cctgatgatg tcgccttcgt cagtgagatt
      241 ccgcacaccg ccaccggcaa gatcctgaag acgcggctgc gcgaggactt caggcagcac
      301 aggctgccga ccgccggctg atatcagtcg gcggcggcgt agggctgcag ctgcggccgg
      361 ccggcgtcgt cccattccag cgtgtaggcc ttgcgccccg gcagcgtgcg cagcagctcg
      421 gcattgcgct cgcccaggtt gttggcccag atcacgcggt cgttcatgtc gggatggctg
      481 atcggcatga agaacacgta atggcgcagc ggcgcgatgc tgttctggta gacgaacggg
      541 cgccgcgcaa agatcaccga gggttccggc agccgcgcgg cgaggtgcag cggaccattg
      601 acgttcgccg cgatgatgcc gacgttgagc acgcgcagcg ggaaataggt gagccatgcg
      661 gcgatcacca gccccagcgc cgtgctcagc agcacacgtg cgccgtcgct gtgcagcggc
      721 ggcgccagcg catccgtgag ccgctgcatg cccaggctca gcagcaggat gaagacggtg
      781 gtggcctcaa gcaaatgcaa cggcccgaag gtatcgattc ccaggtcgga catcaccatg
      841 caggtgcaga actccagcgc cagcgcggcg ctgaacaccc acagccggcg gccggcgagc
      901 agcacggcga gcagcgccag cagcggcagc ggccagccgg aaatgtcggc ggtcagcctc
      961 tgcaggccgg cagtggcagt ttccagcatg cgcccgatgt cggtgaccga cgccagcagg
     1021 taatcgctgt agccgcgaga aagcttgtcg tagccgttgc tcagcgcgtt gccgtagatc
     1081 agctggttca ggtacaggaa cagggtggcg aaggccgcgg cgatgccggc aaacggcgcc
     1141 agcagggtgc ggccggcggc cggcctgctg cgcagcaggt gcaccagcat gagcagcgtg
     1201 gccggggctc cgatcatcac cacggccgac ggccggatga aaaacgccat tgatccacat
     1261 agcgctgcca gcgcggcatc gcgcggcgcg gcgtccgcgt cgtgtgtcag ccgctgcacc
     1321 aggtgcaggt accaggtgag gaagaacagc gcgctggtgt aggccagcag ggtcgcggcg
     1381 gtgaccagcg aggtcatcga cgccgcgtac caggcgagtc ccgcgagcac cgcgggcgcg
     1441 cggcagtagc gttccagcag gcgcgcaagc gggaacacgg tcagcgcgca gagcagggga
     1501 ttgagcagat cgcccatgcc ggcggcaacc gcgggcgcca gcagggccgg ccagccgagg
     1561 aaatactggc tgtgcatgcg gccgtcgttg atcaggaaga cgcggtcata gaattccttc
     1621 agcggtggcg agttctccca caggtgcccg cgcagcagcg agtgggcggc aaacaggtag
     1681 gcgctttcgt cgtcggtaag cggtgcacgc gccagcacga cgaagcgcag gaacagcgcg
     1741 accgcaaaaa tcactgcagc cgtggcccag agcagcg
//
LOCUS       DASZZC010000586         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_51585,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000586 DASZZC010000000
VERSION     DASZZC010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51585"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..659
                     /gene="def"
                     /locus_tag="VHE37_12505"
     CDS             138..659
                     /gene="def"
                     /locus_tag="VHE37_12505"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVT36403.1"
                     /translation="MAVLEILHHPDPRLRQKARPVSVFDAALARLIDDMFETMYAAPG
                     VGLAATQVGVAQRVAVMDCAAEGQPPQPMVMINPEIIESSELEEVEEGCLSVPDYRDR
                     VKRYHRLRMRALDRNGQPYELQAEGLAAQAVQHEIDHLDGKLYIDYLSALKRERLKKK
                     LQKEARARKENAA"
     gene            659..1576
                     /gene="fmt"
                     /locus_tag="VHE37_12510"
     CDS             659..1576
                     /gene="fmt"
                     /locus_tag="VHE37_12510"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVT36404.1"
                     /translation="MKLAFAGTPEFSVAALDALHAAGHSIVAVYTQPDRPAGRGRQLT
                     PTPVARRAAELGIPTHKPLKFDAAAQASLREHAPELMVVVAYGLILPAAALAIPEHGC
                     FNIHASLLPRWRGAAPIQRAILAGDAQTGITIMRMDAGLDTGPMLLRQDIAIGPQTTA
                     AELHDQLAALGARMIVQAVDLLARGQLPEQPQPAEGVTYAGKISKDEARLDWSHPAAE
                     LVRAIHAYNPAPGAWSELNGERLKFFRAAIAEQSGPPGCIISADASGVVVSAGTGAVR
                     ITELQRPGGRVQPAREFLAGRSLAGCTLN"
     gene            complement(1581..>2116)
                     /locus_tag="VHE37_12515"
     CDS             complement(1581..>2116)
                     /locus_tag="VHE37_12515"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HVT36405.1"
                     /translation="DFPQAWRTPANYHDAIAHAAFGVQLVTPADTYCMADRIGKYAVL
                     TLVFTFLTLWLMESLSGHRLHPVQYLFVGAALCLFGLLQLSFAEHFGFEAAFAVATLA
                     VVGMVTLYARSALQRTGRAVLVGGILSGCYAYLYAVLRAEDYALLGGSVALFVALAAA
                     MYLTRKLDWFQPRVAQT"
BASE COUNT          386 a          714 c          700 g          316 t
ORIGIN      
        1 ggcgttataa tgtcccatgg tggcgtcacc cccggccgtt tacgcgattc tggcacaaat
       61 tccagtcgcg gcaaatagtt ggcgtgtaat agtgcagtag tttcgcaaga gcaggtttgc
      121 cggttgtcga atgtgtcatg gccgtactcg aaattcttca tcaccctgat ccgcgcctgc
      181 gccagaaagc gcggccggtc agcgtgttcg atgccgcgct cgcccggctg atcgacgaca
      241 tgttcgagac catgtacgcc gcgcccggcg tcggtctcgc cgccacgcag gtcggcgtgg
      301 cccagcgcgt cgcagtgatg gactgcgcgg cggaaggcca gccgccgcag ccgatggtga
      361 tgatcaatcc cgagatcatt gaatcctccg aactggagga agtcgaggaa ggctgcctgt
      421 cggtgccgga ctaccgcgac cgcgtgaaac gctaccaccg gctgcgcatg cgcgcgcttg
      481 accgcaacgg ccagccctac gaactgcagg ccgaaggcct cgccgcgcag gcggtgcagc
      541 acgagatcga tcacctcgac ggcaagctgt acatcgatta cctgtcggcg ctcaagcgcg
      601 agcgcctgaa gaagaagctg cagaaagagg cccgcgcgcg caaagagaac gcggcctgat
      661 gaagctggcg tttgccggca ccccggaatt ttccgtcgcc gcgctcgatg cgctgcatgc
      721 cgcgggccac tccatcgttg ccgtctacac gcagcccgac cggcctgccg ggagaggacg
      781 ccagctgaca ccgacgccgg tcgcccgccg cgccgccgaa ctgggtattc cgacgcacaa
      841 gccgctcaag ttcgatgccg ccgcgcaggc cagcctgcgc gaacacgcgc ccgaactgat
      901 ggtggtggtg gcctatggcc tgatcctgcc ggccgccgcg ctggcaattc ctgagcacgg
      961 ttgcttcaac atccatgcgt cactgctgcc gcgctggcgc ggcgcggcgc cgatccagcg
     1021 cgccatcctg gccggcgatg cgcagaccgg catcaccatc atgcgcatgg acgccgggct
     1081 cgacaccggt cccatgctgc tcaggcagga catcgccatc gggccgcaaa ccaccgcggc
     1141 ggaactgcat gaccagctcg ctgccctcgg tgcccgcatg attgtgcagg ccgtcgacct
     1201 gctggcccgc ggccagttgc cggagcagcc gcagccggcg gaaggcgtga cctacgccgg
     1261 caagatcagc aaggacgaag cgcggctgga ctggtcgcat cctgctgccg aactggtgcg
     1321 cgcgatccat gcgtacaacc ccgcaccggg cgcgtggagc gagctcaatg gcgagaggct
     1381 gaaattcttc cgcgccgcta tagccgaaca gtccgggcca ccgggctgca tcatttccgc
     1441 cgacgcatcc ggcgtggtgg tctctgcagg aaccggcgcc gtacgcatca ccgagctgca
     1501 acgtcccggc gggcgcgtac agccagcgcg ggaatttttg gcgggacgat cgctcgccgg
     1561 atgcacgctg aactagcgcg tcaggtctgc gcgacgcgcg gctggaacca gtccagcttg
     1621 cgcgtgagat acatcgccgc tgcgagcgcg acgaacaagg cgaccgaacc gcccagcagc
     1681 gcgtagtcct cagcgcgcag cacggcatac aggtaggcgt agcagccgct gaggatgccg
     1741 ccgaccagca ctgcacgccc ggtgcgctgc agcgcactgc gcgcatacag ggtgaccatg
     1801 ccgaccacgg ccagcgtggc gacggcaaag gccgcctcga atccgaagtg ctcggcgaat
     1861 gacagctgca acaggccgaa caggcacagc gccgcgccga cgaacaggta ttgcaccggg
     1921 tgcaggcggt ggccggacag gctttccatc agccacagcg tgaggaaggt gaagaccagt
     1981 gtcagcacgg cgtacttgcc gatgcggtcg gccatgcagt aggtgtcggc cggtgtcacc
     2041 agctgcacgc cgaaggccgc atgcgcgatg gcgtcgtggt agttcgccgg cgtgcgccag
     2101 gcctgcggga aatcgc
//
LOCUS       DASZZC010000587         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_561200,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000587 DASZZC010000000
VERSION     DASZZC010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561200"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VHE37_12520"
     CDS             complement(<1..723)
                     /locus_tag="VHE37_12520"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HVT36406.1"
                     /translation="MNASSLIRSLGVVALICASAAAAAPAGGQVVAGQASISATPNLT
                     TITQGSNATIINWQQFNIGRGEQVTFIQPTAQSTALNRVIGGSAAQIFGSLTANGRVF
                     LVDPQGVVFGRTAVVDVGGLLASTLDIGDTDFMGGRYDFSSAGVGAVSNAGRLQARDG
                     GFVVLAGSQVNNAGLVRARLGKTVLAAGSALTLDFDGDGLISYAVMRGADGAQVLNTG
                     RIAADGGLVALSAQAAGDAVQAV"
     gene            complement(735..2294)
                     /locus_tag="VHE37_12525"
     CDS             complement(735..2294)
                     /locus_tag="VHE37_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HVT36407.1"
                     /translation="MGSRLLLLVMVSVGALSFARAACAQLPAPADVVDQARSAGGDER
                     VTVQSFEFSGNTVYPASVLMPLLVTYLNRPLSLEELYAAADAVTAYYRMHGYTLAAVQ
                     LPAQDISSGKVQLEVVEGRVGGVAVTGAHRYDPAELLRTLDLPRGSVYRGDTLERGLF
                     RLNDLPGLSARAVLQPGADFGTSDVIVNVDERLLAGALSVDNYGRKNIGEFRVGGTGT
                     LDNPLGVADQLQLIALHAEADLLNYGYGEYSLPLTTAGQRVAVSYGKALFKVTSAGIA
                     GDNSKFRTGVRWPLAHTRADNFLLNFGGSYTHANADLSGLSISDTRITLFDASLLYSH
                     RYDNSAASQAQLSLGTDLRRQTRADLLSDGVRHERARIELDAQHLQPLWPRGLALWAR
                     VDGVWSPDPLADTEQFAIGGPDSVRAYPASEVRGDRGYQGSLGLQQLFRFGPVDLQLR
                     EFADAGEAYVVHPLPGTDSKTSLAGYGIGFDLHWRMLSFKLDGARPIHARPSSDGRDS
                     GRLFGAIAVAY"
     gene            complement(2497..2573)
                     /locus_tag="VHE37_12530"
     tRNA            complement(2497..2573)
                     /locus_tag="VHE37_12530"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2537..2539),aa:Asp,seq:gtc)
     gene            complement(2585..2660)
                     /locus_tag="VHE37_12535"
     tRNA            complement(2585..2660)
                     /locus_tag="VHE37_12535"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2625..2627),aa:Val,seq:tac)
     gene            complement(2700..2972)
                     /locus_tag="VHE37_12540"
     CDS             complement(2700..2972)
                     /locus_tag="VHE37_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVT36408.1"
                     /translation="MNKTELIAAIAAKADLSKVDASNAVEAFFEVVAKALKKKDKVAL
                     VGFGTFMVRERKARTGRNPKTGAPLKIKASKTPAFKSGKALKDAIK"
BASE COUNT          661 a         1133 c         1057 g          601 t
ORIGIN      
        1 caccgcctgc acggcatcgc ccgccgcctg cgcggacagc gccaccagtc cgccgtcggc
       61 ggcgatgcgg ccggtattga gcacctgcgc gccatccgcg ccgcgcatca cggcgtagct
      121 gatcaatccg tcgccatcga aatccagcgt aagcgcagaa ccggccgcca gcaccgtctt
      181 gcccaggcgc gcgcgcacca ggccggcatt gttcacctgg ctgccggcca gcacgacaaa
      241 gccgccatcg cgcgcttgca ggcggccggc gttactcacc gcgcccacac cggcgctact
      301 gaaatcgtag cggccgccca tgaaatcggt gtcgccgatg tccagcgtcg atgccagcag
      361 gccgcccacg tcgaccactg ccgtgcggcc aaacaccacc ccctgcggat cgaccaggaa
      421 cactcggcca ttggcggtga gcgagccgaa tatctgagca gccgagccgc cgatgacccg
      481 gttgagcgcg gtgctttgtg ccgtcggctg gatgaaggtc acctgttcgc cgcggccgat
      541 gttgaactgc tgccagttga tgatggtcgc attgctgcct tgcgtgatgg tagtgagatt
      601 cggcgtggcg gaaatgctcg cctgcccggc caccacctgc ccgccggccg gtgccgctgc
      661 agcggcgctt gcgcagatca gggccaccac gcccaggctg cgaatcaggc tggaagcgtt
      721 catgagccgt cctcctagta cgccaccgca atcgcgccga acaagcggcc gctgtcgcgt
      781 ccgtcgctgc tgggccgtgc gtggatgggc ctggcgccgt caagcttgaa actgagcatg
      841 cgccagtgca ggtcgaagcc gatgccataa ccggccagcg acgtcttgct gtccgttccg
      901 ggcagcggat gaaccacgta agcctcgcca gcatcggcga actcgcgcag ttgcagatcc
      961 accggcccga aacggaacaa ttgctgcagg cccagactgc cctggtagcc gcgatcgccg
     1021 cgcacttccg acgctggata ggcacgcacg ctgtctggac cgccaatggc gaactgctcg
     1081 gtgtcggcca gcggatcggg cgaccacaca ccgtccacgc gcgcccatag cgcgagtccg
     1141 cgcggccaca atggctgcag gtgctgcgcg tcgagctcaa tgcgcgcacg ctcgtgccga
     1201 acgccgtcgc tgagcaggtc cgcccgcgtc tgccggcgaa ggtcagtgcc cagcgacagc
     1261 tgagcctggc tggcggcgga attatcgtag cgatggctgt agagcagcga ggcgtcgaac
     1321 aacgtgatgc gcgtgtcgct gatggaaaga ccactcaggt cggcattggc atgcgtatac
     1381 gagccgccga agttcagcag aaaattgtcg gcgcgcgtgt gcgccaacgg ccagcgcacg
     1441 cctgtgcgga atttgctgtt gtcgccggcg ataccggcgc tggtcacctt gaacaaggcc
     1501 ttgccatagg acaccgcgac gcgctgcccg gcggtggtca gcggcaggct gtattcgcca
     1561 tagccgtaat tgagcagatc ggcctcggca tgcagcgcga tcagctgcag ctggtcggcg
     1621 acacccaggg ggttgtcgag cgtacccgtg ccgccgacgc gaaactcgcc gatgttcttc
     1681 cgtccatagt tgtcgacgct cagagcgccg gccagcagcc gttcatcgac attgacgatg
     1741 acgtcgctgg tgccgaagtc ggcgcccggc tgcagcacgg cccgcgccga caatcctggc
     1801 agatcattca accggaacaa tccgcgttca agagtatcgc cgcgatacac gcttccgcgc
     1861 ggcaagtcga gcgtgcgcag caactccgca gggtcgtagc gatgggcacc cgtcaccgca
     1921 acaccgccca cgcgcccttc aaccacctcc agctgcacct tgccgctgct gatgtcctgg
     1981 gccggcaact gcacggctgc aagggtgtag ccgtgcatgc ggtagtacgc cgtcaccgcg
     2041 tcggcggcag catagagttc ctccagcgat agaggacggt tgagataggt gacgagcaag
     2101 ggcatcaaca cgctcgcggg ataaaccgtg ttgccgctga actcaaacga ctggacggtc
     2161 acccgctcat caccgccggc gcttcgcgcc tgatcgacga catcggctgg cgcaggtaat
     2221 tgggcgcatg cagcgcgcgc gaaactcagc gcaccaacgc tgaccatgac cagcaatagc
     2281 agacgactcc ccatcgtgca ctccgcccgg ctaaaggcgc gacgcgcgta gccgtgtgcc
     2341 ccgactatag gcaggggcgc gaaccgaggg caaacccgat cccgcgcggg aacgtgagct
     2401 tgctcaccaa cggtaggttt tccgtggtcg cgccgcagcg caaccttgaa cggcagacgc
     2461 aaaaaaggcg ccttgcggcg ccttctgctt ccaacatggc ggagcggacg ggactcgaac
     2521 ccgcgacccc cggcgtgaca ggccggtatt ctaaccgact gaactaccgc tccacggtct
     2581 tgattggtgg gtgctgacgg gctcgaaccg ccgacattcg ccttgtaagg gcgacgctct
     2641 accagctgag ctaagcaccc ttgagcgggc cgttcgacat cagccccaac aaagaaaaac
     2701 tattttatag cgtccttgag cgctttgcca gatttgaatg cgggcgtctt gcttgccttg
     2761 atcttcagcg gcgcgccggt cttcggattg cggccggtgc gcgccttgcg ctcgcggacc
     2821 ataaaggtac caaagcccac cagcgccacc ttgtccttct tcttgagcgc ttttgcaaca
     2881 acctcgaaga aagcttccac ggcattgctg gcgtcgacct tggacagatc ggccttggcc
     2941 gcgatcgcgg caatcaattc ggtcttgttc atgagtgaat tcctacgtgc tgaaaatgat
     3001 gagtggctcc ggcgcaagcg ccgctgccga tgggactgga aagctgggcc gcaagggtcg
     3061 ccgacgcgac ctgattttcg tagaaaaata aagcggcgaa aattcgcggc gctttatagc
     3121 aagcaggttt tttagcgtca acaaatcgcg atgccgttgc cactcaaaat tggaaaaaca
     3181 ggggaaaaca ctcttgacca cattttgtcc gctggctagt ttccttcgct gtgtgggagt
     3241 tttcagcctg atctgcactc caggaggaac tgcgaccggg ccagcacagc aactcagaag
     3301 ttcttcttca tagggagttc atcatgtttt tcaaatcggc cggccgtctg ctcgcaggcg
     3361 gcatacttgg cattgccgca acgtcgtcca ttgcatacgc acaggatgca gcaactcccg
     3421 accaggctgc cccggtagcg cctgcggatg cg
//
LOCUS       DASZZC010000588         5264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_371542,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000588 DASZZC010000000
VERSION     DASZZC010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371542"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2476)
                     /gene="infB"
                     /locus_tag="VHE37_12545"
     CDS             complement(<1..2476)
                     /gene="infB"
                     /locus_tag="VHE37_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HVT36409.1"
                     /translation="MSNVTVQDFANELRRPVTELLAQFKEAGVSVGSAEAEITGADKL
                     ALLNYLKQKTRGTVSAAPLSTAGSASRITLKRKETTELKLGGGRGAPSKTVSIEVRKK
                     RTIVKPVPGEVDAEAAAAAEAAAAKEEAEREAAARAAAEAEAKRHQEELERKLRAEEE
                     ARLVAEAEENARRNKEEHENRLKNDPIYKARWDAEQSRKRAEENARRVAESNKATAAA
                     APAAAATAAKKPEPKSARELEKERDRHRQELHVAEGKSGKREKKSKKPSGKIKVENVH
                     GFEKPTAPVIREVEVPEAITVGELANRMAVKAGDLIKVMMKNGVMATINQSLDQDTAL
                     LMVEEMGHKARAVKATDAEESLEQAVTGEQKEVATELRPPVVTIMGHVDHGKTSLLDY
                     IRKTKVAAGEAGGITQHIGAYHVKTPKGVISFLDTPGHAAFTRMRARGAQITDVIILV
                     VAGDDSVMPQTREAIQHARSAKAPMVVAVTKIDKHDADLNRVKNDLLKEEVVAEDFGG
                     DVQVVGVSSVSGQGIDDLLDAVLLQAEVLELKAPVDAPARGAIIESSLEKGRGAVATV
                     LVRSGTLHQGDVILTGPYFGRVRAMFDEAGKPVKEAGPSIPVQVLGLSGVPDAGDDVV
                     VVADERKARELALLRESKAREQKLAASSTVRMEQIFARMGEGEQKNLNLMIKADVQGS
                     AEAIADALRKLPSAEVKVNVLSSGIGGISESDVDLALASRAIIIGFNVRADGGARRRI
                     QDTGVDVRYYSIIYEIIDDVSSAISGLLGTETREQIVGIAQVRDVFRSSKFGNIAGCL
                     VIEGSVQKNLPIRVLRNQTVIYEGVLE"
     gene            complement(2499..3986)
                     /gene="nusA"
                     /locus_tag="VHE37_12550"
     CDS             complement(2499..3986)
                     /gene="nusA"
                     /locus_tag="VHE37_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HVT36410.1"
                     /translation="MNKNILLMVDVVSNEKAVEKEIIFQALEAALASATKERYGDESD
                     IRVAIDRATGEYESFRRWTVLDDESEDFEFPERQIKVTYAQKRDENLKAGDVIEEKVE
                     SVDFGRIGAQKAKQVIVQKVREAERAQVVEAYKDRVGELITGMVKRLERGAVILDLGG
                     NAEAIIPREHLIPREPARPGDRMRGYLYEVSPQQRGPQLFLSRTSPKFLMELFKLEVP
                     EIAQGLIELKGAARDPGLRAKIAVQAKDKRIDPVGACVGMRGSRVQSVSNELAGERVD
                     IVPWDDNIAQFVINAMAPAEVENIVVDEESHSMTIGVAEDKLAQAIGRGGQNVRLASE
                     LTGWVLNVMTAAEADAKKESENQNVQQMFQEALDVDAEVASILVQEGFTTLEEIAYVE
                     AAELAQIEEFDENIVEELRNRARDVLLTKEIAKAERAADTKPADDLLAVEGMDEDTAY
                     ALAGAGVVTAENLADLATDELTEMVKMDETRASALIMAARSKVYA"
     gene            complement(3986..4456)
                     /gene="rimP"
                     /locus_tag="VHE37_12555"
     CDS             complement(3986..4456)
                     /gene="rimP"
                     /locus_tag="VHE37_12555"
                     /inference="COORDINATES: protein motif:HMM:NF000927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HVT36411.1"
                     /translation="MSLLHEKLLSLLEPAIEALGYELVLLEFVPQPGSALLRLYIDSP
                     GGVNLDDCTRVSREVAGVLDVEDPISSAYNLEVSSPGLDRPLVKPAHYERFLQHVAKA
                     TLIAPRNGRRRFSGRIAAVNAQGVTLETAEGPVELKYSEIEKARLVPDYEQELH"
     gene            complement(4549..4625)
                     /locus_tag="VHE37_12560"
     tRNA            complement(4549..4625)
                     /locus_tag="VHE37_12560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4589..4591),aa:Met,seq:cat)
     gene            complement(4940..5024)
                     /locus_tag="VHE37_12565"
     tRNA            complement(4940..5024)
                     /locus_tag="VHE37_12565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4988..4990),aa:Leu,seq:gag)
     gene            complement(5069..>5264)
                     /locus_tag="VHE37_12570"
     CDS             complement(5069..>5264)
                     /locus_tag="VHE37_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HVT36412.1"
                     /translation="ATVFFSVSLALAYIVHGRAAPKSVTDSIKQEVPVPTAVVPAPTA
                     PPATSAASPAPPAETPKVPE"
BASE COUNT          753 a         1808 c         1642 g         1061 t
ORIGIN      
        1 attccagcac gccttcgtag atcaccgtct ggttgcgcag cacgcggatc ggcaggttct
       61 tctgcacgct gccctcgatc accaggcagc cggcaatgtt gccgaacttg ctggagcgga
      121 acacatcgcg cacctgcgca atacccacga tctgctcacg cgtctcggtg ccgagcaggc
      181 cggagatggc cgagctcacg tcatcgatga tctcgtagat gatcgagtag tagcgcacgt
      241 cgacgccggt gtcctggatg cgccggcgcg cgcctccatc ggcacgcacg ttgaagccga
      301 tgatgatcgc gcggctggcc agtgcgaggt cgacgtcgga ttcgctgatg ccgccgatgc
      361 ccgacgacag cacgttgacc ttcacttccg cgcttggcag cttgcgcagc gcgtcggcga
      421 tggcttccgc cgaaccctgc acgtcggcct tgatcatcag gttgaggttc ttctgctcgc
      481 cctcgcccat gcgcgcgaaa atctgctcca tgcgcacggt ggaagacgcg gccagcttct
      541 gctcgcgcgc cttcgattca cgcagcagtg ccagttcgcg cgccttgcgc tcgtcggcca
      601 ccaccaccac gtcgtcgccc gcgtcgggca cgcccgacag gcccagcacc tgcacgggaa
      661 tggacgggcc ggcttccttc accggcttgc cggcttcgtc gaacatggcg cgcacgcggc
      721 cgaagtacgg gccggtgagg atgacgtcgc cctggtgcag cgtgccgctg cgcaccagca
      781 cagtggccac ggcgccgcgg cccttttcca gcgaagactc gatgatcgcg ccgcgcgccg
      841 gtgcgtcgac cggagccttg agctccagca cttcggcctg cagcagcacg gcgtcgagca
      901 ggtcgtcgat accctggccg ctgaccgacg acacgccgac cacctgcacg tcgccgccga
      961 aatcttcggc caccacttct tccttcagca ggtcgttctt gacgcggttg agatcagcgt
     1021 cgtgcttgtc gatcttggtc acggccacaa ccatcggcgc cttggcgctg cgcgcgtgct
     1081 ggatggcctc gcgcgtctgc ggcatcacgc tgtcgtcgcc ggcgaccacc aggatgatga
     1141 cgtcggtgat ctgcgcgccg cgcgcgcgca tgcgggtgaa cgcggcgtgg cccggcgtat
     1201 caaggaagct gatcacgccc ttgggcgtct tcacgtggta cgcgccgata tgctgggtga
     1261 tgccgcccgc ctcgcccgcc gccaccttgg tcttgcggat gtaatcgagc aggctggtct
     1321 tgccgtggtc gacgtggccc atgatggtga ccaccggcgg ccgcagctcg gtggcgactt
     1381 ccttctgctc gccggtgacc gcctgctcca ggctctcctc ggcgtcggtg gccttgaccg
     1441 cgcgcgcctt gtggcccatt tcctcgacca tcagcagcgc ggtgtcctgg tccagcgact
     1501 ggttgatggt cgccatgacc ccgttcttca tcatcacctt gatcaggtcg ccggccttca
     1561 ccgccatgcg gttggccagt tcgcccacgg tgatcgcctc gggcacttcc acctcgcgga
     1621 tcaccggtgc ggtcggcttc tcgaagccgt gcacgttctc caccttgatc ttgcccgacg
     1681 gcttcttgct cttcttctcg cgcttgccgg atttgccctc ggcaacgtgg agttcctggc
     1741 ggtgacggtc gcgttccttt tccagttcgc gcgcgctctt gggctcaggc tttttggctg
     1801 ctgttgctgc agccgccggc gccgcggctg cggtcgcctt gttcgattcg gccacgcgtc
     1861 gcgcattttc ctcggcgcgc ttgcgcgact gctcggcatc ccagcgtgcc ttgtagatcg
     1921 ggtcgttctt gagccgattc tcgtgctctt ccttgttcct gcgcgcgttt tcctcggctt
     1981 ccgccaccag gcgcgcctct tcctcggcgc gcagcttccg ttccagttct tcctggtggc
     2041 gcttggcctc ggcctcggcc gcggcacgcg cagcggcctc gcgctcggct tcttccttgg
     2101 cagcagccgc ttcggcggcg gcagcagcct cggcatccac ctcgcccggc accggcttga
     2161 cgatggtgcg cttcttgcgc acctcgatgc tcaccgtctt cgacggcgcg ccacgcccgc
     2221 cgccgagctt gagttcggtg gtttccttgc gcttgagcgt aatgcggctg gcactgccgg
     2281 cggtcgacag cggcgcggcg ctgaccgtgc cgcgggtctt ctgcttgagg taattgagca
     2341 gggcgagctt gtcggcgccg gtgatctcgg cttctgcact gccgacgctg acgccggcct
     2401 ccttgaactg cgcgagcagt tccgtcaccg ggcggcgcag ctcgttggca aaatcctgta
     2461 cggtcacgtt cgacatagct ctccccgcgc gtggtgcttc aggcgtagac cttgctgcgc
     2521 gcggccatga tcagggccga cgcgcgggtc tcgtccatct tcaccatctc ggtcaattca
     2581 tcggtggcca gatcggccag gttctcggca gtgacaacgc cggcaccggc cagcgcatag
     2641 gcggtgtcct cgtccatgcc ctcgaccgcc agcagatcgt cggccggctt ggtgtcggcg
     2701 gcgcgctcgg ccttggcgat ttccttggtc agcagcacat cgcgcgcgcg gttgcgcagt
     2761 tcctcgacga tgttctcgtc gaattcctcg atctgcgcca gctcggccgc ctcgacgtag
     2821 gcgatctctt ccagcgtggt gaagccttcc tgcaccagga tcgaggcgac ctcggcgtcg
     2881 acgtcgagcg cctcctggaa catctgctgc acgttctggt tctcgctttc cttcttggcg
     2941 tcggcctcgg ccgcggtcat tacgttgagc acccagcctg tgagttccga cgccaggcgc
     3001 acgttctggc cgccgcggcc gatggcctgc gccagcttgt cctcggccac gccgatggtc
     3061 atcgagtgag actcctcgtc gaccacgatg ttctcgactt ccgccggggc catggcgttg
     3121 atcacgaact gcgcgatgtt gtcgtcccac ggcacgatgt cgacgcgctc gcctgccagc
     3181 tcgttggaga cgctctgcac gcgcgaaccg cgcatgccca cgcaggcgcc gaccgggtcg
     3241 atgcgcttgt ccttggcctg caccgcgatc ttggcgcgca ggccggggtc gcgcgcggcg
     3301 cccttcagtt cgatcaggcc ctgcgcgatt tccggcactt ccagcttgaa cagttccatc
     3361 aggaacttgg gcgaggtgcg cgacaggaac agctgcgggc cgcgctgctg cgggctcact
     3421 tcgtagagat agccgcgcat gcggtcgccc gggcgtgccg gctcgcgcgg gatcaggtgc
     3481 tcgcgcggga tgatggcctc ggcattgccg ccgagatcga ggatgacggc accgcgttcc
     3541 aggcgcttga ccatgccggt gatcagttcg ccgacgcggt ccttgtaggc ttcgaccacc
     3601 tgcgcccgct cggcctcgcg cactttctgc acgatgacct gcttggcctt ctgcgcgccg
     3661 atgcggccga agtcgaccga ctcaaccttc tcctcgatga cgtcgccggc cttcaggttc
     3721 tcgtcgcgct tctgcgcgta ggtcaccttg atctggcgct cggggaactc gaagtcttcg
     3781 ctttcatcat ccagcacggt ccagcggcgg aacgattcgt actcgccggt cgcgcggtcg
     3841 atggcgacgc ggatgtccga ttcgtcgccg tagcgttcct tggttgcgga cgccagcgcg
     3901 gcttccagcg cctggaagat gatttccttc tcgaccgcct tctcgttgga gacgacgtcc
     3961 accatcagca agatgttctt gttcatcagt gcaattcctg ctcgtagtca ggcaccaagc
     4021 gcgccttttc gatttccgaa tacttcagtt ccaccggccc ctcggccgtc tccagcgtca
     4081 caccctgcgc attgaccgcg gcgatgcggc cggaaaaacg gcgccgcccg ttgcgcggag
     4141 cgatcagcgt ggccttggcc acgtgctgca gaaaacgctc gtagtgcgcc ggcttcacca
     4201 gcggccggtc gagcccgggc gaggaaactt ccaggttata ggcagagctg atcgggtctt
     4261 ccacgtcgag cacgccggcc acttcacggc tgacgcgcgt gcagtcgtcc aggttcaccc
     4321 cgccgggtga atcgatgtac aggcgcagca gggcggaacc gggctgcggc acgaactcga
     4381 gcagcacgag ctcgtagccc agcgcttcga tcgcgggctc cagcaagctc agcagcttct
     4441 cgtgcagcag gctcattgca tttccatcgc caacaaaaaa gggcctcaag gggccctttt
     4501 gaaagcctga aactcccggc ccaagcgccg aaaattcctt gaaataattg gtagcggggg
     4561 caggatttga acctacgacc ttcgggttat gagcccgacg agctgccaga ctgctccacc
     4621 ccgcaccagg ggcgcgcagt atagtgatgt ccaccaactg ccgcaacaaa atagtagggt
     4681 tgcagcggga gttttcaatg gcatcggtag atgccgggaa caccgaaacg gcgcgctttc
     4741 ccctttgccg ccgcgggaga gagaatttcg ccttgcagtg cctgccgcgt aagttctcaa
     4801 cggcagcagt tccgatctca gactccggga cccctctctc ttctctctcc cgcaagggga
     4861 gagagtattt cgccttacgg cgcctgcggc gccgctgatc tgcttaaata aaaaaaccgc
     4921 cagcggcggt ttttttattt ggtgcccacg gagggactcg aacccccacg ccgttaagcg
     4981 ctaccacctc aaggtagtgc gtctaccaat tccgccacgt gggccaatac tggagggcgc
     5041 gcagtgtaac ggcgcccgga tacgcgtgct actccgggac cttcggcgtt tccgccggcg
     5101 gggccgggct ggcggcggat gttgccgggg gcgcggtcgg cgccgggacg accgccgtcg
     5161 gcacgggaac ttcctgcttg atggagtcgg tcaccgactt gggcgccgcg cggccgtgca
     5221 cgatgtaggc cagcgccagg ctcacgctga agaacaccgt ggcc
//
LOCUS       DASZZC010000589         6750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_814845,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000589 DASZZC010000000
VERSION     DASZZC010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814845"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1010
                     /locus_tag="VHE37_12575"
     CDS             78..1010
                     /locus_tag="VHE37_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36413.1"
                     /translation="MQSKQFHHPLTGGGSLCIHRWLPDGAGEVKSAVLIVHGMAEHGA
                     RYARLAQALTDAGHAVYAPDLPGHGRTARAPDELGHVSSRGGWLKLLSAINAVRELVQ
                     HQLHDPPIYLLGHSMGSMLLQDYLVEHGHNLAGAIFSATTGDLGGLRPVAVALLKAEA
                     AWYGRAHRSALAEALTFKDFNRRFRPARTGFEWLSRDAAEVDKYVSDPACGFRCSCAL
                     WIDLFTAGAGWTDAQRLARIPKSLPVLLIGGSEDPATRGEAGPRALERSYQQAGIRDV
                     SVRIWPGARHELLNETCRDEVTAALLEWLAQRSR"
     gene            complement(943..1713)
                     /locus_tag="VHE37_12580"
     CDS             complement(943..1713)
                     /locus_tag="VHE37_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36414.1"
                     /translation="MLQQPGRNIINDRSRKGSADALPPRSTAHRRIAAIEPVLPAEAG
                     AHRNRARVLRAALELFAHRGYGGTSVRDICAAAHMQAPTLYAQFPSKEHVLAELIRLA
                     YAELLRRLRAALLDCQPDPAQQLTALVRAHVIAHCEMAMLGVVASAELHMLSAGAAAP
                     ILVMVEQAEQLLIDIFTRGRRQGLFEVADEFLALRAIASLGVRVANWYTPACGRTPVQ
                     IADAYAGFAQRLLVAPLSGCAAPATPAARRSLRRGRSH"
     gene            1806..4187
                     /locus_tag="VHE37_12585"
     CDS             1806..4187
                     /locus_tag="VHE37_12585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36415.1"
                     /translation="MGTACLLLACAAQAQDAGTTGAQPPPVIPVAAPAEAAPQAAGDK
                     TGDAVQLGDVVVTATKREKSLREVPASIRELDGRDLEAKAVLSINELLDSTPGVTSNS
                     TRPGEERIIMRGISTTASGTTVAPYPVGVFVGDTSFNEPYAASITPDLSAFDLAGVDI
                     LKGPQGTLFGGAALAGAIRYRLNDPLTDQWQLRAFSQFTNLEHGSNALTEGVVVNAPL
                     RVEDGGLGARLVYIDRQYPGSIDWTRPDPPQRDANQGRGEQFRGSLLWTPTDAARLQF
                     TYLEQDYSAANDIIVADGPYGPRSSSASLQPWPNQHRFALYQLGGQYDFEHVRIVSST
                     SRTEKKRYNSIESLGALLGSPPGGTPAALALPFITHQDSTSFSQELRVQSIDTPLEWL
                     VGGYYYHAPINYELVLNVQALQDLGGQANNLAPLLAQLRQAYGALYGANNELNTLLGQ
                     LDSVTGNPGCELSVLCAETHAKATEKALFFDLSRKFWQRLELEAGARLYRTEVNGGFV
                     GTGAGARLVNNGMSPVYVLNDLVEQGINPKFTATLHFTRDISTYFQAARGFRFGGIQQ
                     IPADKVQNVPGTFKSDSLWNYELGLRTAWLGNTLHADVTGFHIDYKNPQVQLKDLEEL
                     NYYDNVGAAVSNGVEGSLEWLTPLTGIALSATGGYTNAHTTEPFKAGNTPVPAGVPLP
                     GSAKVQYSGQLTYLWPYGGDYSWGGALSYVYVGKTSPGLQVSQYVYDFGVVNFSSQFG
                     APGWPGKPSLALSIENLADRTAPVSIIGSATGSSYYLLTPPRTYTARLTVEFD"
     gene            complement(4198..5142)
                     /locus_tag="VHE37_12590"
     CDS             complement(4198..5142)
                     /locus_tag="VHE37_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HVT36416.1"
                     /translation="MRVGIAIDVSTDVSHEFVVQNHIHVLPSTVHMNGHSVVYDRDPE
                     KGALFYRDHLGDKGIDAETTPFSVKEIEDLFLKRLVLEYDYVFLITLMATRSLTFDNA
                     HKASFTILQSYDSVRNAKSVPGPFALRVVDSESLFTGPGVLAWEAVRMAREGASPVDI
                     RKRLDDLIPHTYAYIVPDDLYYIHARTARRGDHSVGWAAYIIGNALDIKPIIRANRGE
                     TSPVAKVRHHDKAVEKLFLHGAAQIRRGLLVPMVNISYGGDPLAVPKMPGYGELARAA
                     TEKGVELMLSMMAPAGAVNVGGGAITMAYCARDASEFE"
     gene            complement(5373..6104)
                     /locus_tag="VHE37_12595"
     CDS             complement(5373..6104)
                     /locus_tag="VHE37_12595"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVT36417.1"
                     /translation="MNYREPQLRRMLAAEYVLGTLQGRARMRFDRLLRADAALLGEVH
                     AWERKLAPLALAITPVPPRGIVWSGIERRINAMNTPTLPPRKLPSINFWRTWSAVATA
                     AALLLAVALQQQMQFSMQIVVMPKIVHEAPLMPYVAVLKPDKSDARWVVSMYPEKKTM
                     KVAASGSYPVDAQHALELWALEKSGPRPLGLLPLQGQAEMALPAAMQVEGELTLAVSV
                     EPTGGSPTGLPTGPVILSAPASRAI"
     gene            complement(6101..6655)
                     /locus_tag="VHE37_12600"
     CDS             complement(6101..6655)
                     /locus_tag="VHE37_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT36418.1"
                     /translation="MDDVQPLAQLLAATAAGDQRAFRELYAATSAHLYGLLLRMLKRR
                     DWAEDALQDCYLKIWQKAETYEPAKGVPLAWLSTVARYRALDLLRVKRPEVELPEESE
                     APPLVLADDGQSPEAGAIEGEGVGRLNDCLRGLQPEQRSSVLLAYYEGYTHHELAARM
                     KAPLGTVKSWVRRGLARLRECLDA"
BASE COUNT         1104 a         2298 c         2252 g         1096 t
ORIGIN      
        1 cctggtcgat cgcaagcagc gcggtcattg gcgtgctctc ctcggttgtg tcatttttgc
       61 cgcgggagaa cttaaccgtg cagagcaaac aattccacca cccgctcacc ggcggcggca
      121 gcctgtgcat ccaccgctgg ctgcccgacg gcgccggcga agtgaagtcg gccgtgctca
      181 tcgtgcacgg catggccgag cacggcgcac gctatgcgcg cctggcgcag gcgctgacgg
      241 acgcgggcca cgcggtctac gcgcccgatc tgcccgggca tggacgcacg gcgcgcgcgc
      301 ccgatgaact cggccacgtc agcagccgcg gcggctggct caagctgctg tcggcgatca
      361 acgcggtgcg cgaactggtc cagcaccagc tgcacgatcc gccgatctat ctcctcggcc
      421 actcgatggg ctcgatgctg ctgcaggact acttggtcga gcacggccac aatctcgcgg
      481 gcgcgatctt ttcggccacc accggcgacc ttggagggtt gcgtcccgtc gcggtcgcgc
      541 tgctcaaggc cgaggcggcc tggtacggac gcgcacaccg cagcgcgctg gccgaggcgc
      601 tcaccttcaa ggatttcaac cgtcgcttcc ggccggcacg caccggcttc gaatggctgt
      661 cgcgcgatgc ggcggaagtg gacaagtacg tgagcgatcc cgcctgcggc ttccgctgca
      721 gctgcgcgtt gtggatcgac ctgttcaccg ccggcgccgg ctggaccgac gcgcagcgcc
      781 tggcgcgcat tcccaagagt ctccccgtgc tgctgatcgg cggcagcgag gacccggcga
      841 ccaggggcga agccgggccg cgcgcgctgg agcgcagcta ccagcaggcg ggcatccgcg
      901 acgtgagcgt gcgcatctgg cccggcgcgc ggcacgaatt gctcaatgag acctgccgcg
      961 acgaagtgac cgccgcgctg ctggagtggc tggcgcagcg cagccgctga gcggcgccac
     1021 cagcaggcgc tgcgcaaacc cggcgtaggc atcggcgatc tgcaccggcg tgcgcccgca
     1081 ggccggcgtg taccagttgg ccacacgcac gcccaggctg gcgatggcac gcagcgcaag
     1141 gaattcgtcg gccacttcga acaggccctg gcgccggccg cgcgtgaaga tgtcgatcag
     1201 cagctgctcg gcctgttcga ccatcaccag gatcggcgcg gcggcgcctg ccgacagcat
     1261 gtgcagctcg gcgctggcca ccacgccgag catggccatc tcgcaatgcg cgatgacatg
     1321 cgcgcgcacc agcgcggtga gctgctgcgc cgggtccggc tggcagtcca gcagcgcggc
     1381 gcgcagccgc cgcagcagtt cggcataggc cagccggatc agttcggcca gcacgtgctc
     1441 cttcgacggg aactgcgcgt acagcgtcgg cgcctgcatg tgcgcggcgg cgcagatgtc
     1501 gcgcaccgag gtgccgccgt agccgcggtg cgcgaacagt tccagcgcgg cgcgcagcac
     1561 gcgcgcgcgg ttgcggtggg cgccggcttc ggcgggcagc accggctcga tggcggcgat
     1621 gcgccggtgc gcggtcgagc gcggcggcag tgcatcggcg gaaccttttc ttgaacgatc
     1681 gttaataata tttctcccag gttgctgcag cattttacct gccgatctgt tcccggtata
     1741 ttaacgaacg ttaaagagac tgcggaccac cgctgcaacg aggagaacac atgcggagcc
     1801 atagcttggg gacggcctgc ctgctgctgg cctgcgctgc gcaggcgcag gatgccggca
     1861 cgacgggcgc tcaaccaccg ccggtcatcc cggtggcggc gccggctgaa gccgcgccgc
     1921 aagccgccgg cgacaagacc ggcgacgccg tgcaattggg cgacgtggtg gtcaccgcta
     1981 ccaagcgcga gaaatcgctg cgtgaagtac cggcgtcgat ccgcgaactc gacggccgag
     2041 acctggaagc gaaagccgtg ctgtcaatca acgagctgct cgacagcact ccgggcgtca
     2101 cctccaattc cacgcgaccg ggagaggagc gcatcatcat gcgcggcatt tccaccaccg
     2161 cctcgggcac cacggtggcg ccgtatccgg tgggcgtgtt cgtcggcgac acctcgttca
     2221 acgaacccta cgcggccagc atcacgccgg acctgtcggc gttcgatctc gccggcgtcg
     2281 acatcctcaa gggtccgcag ggcacgttgt tcggtggtgc ggcgctggcc ggcgccatcc
     2341 gctaccggct gaacgatccg ttgaccgacc agtggcagct gcgcgcgttc tcgcagttca
     2401 ccaacctgga gcatggcagc aacgcgctga ccgagggcgt ggtggtcaac gcgccgctgc
     2461 gcgtggaaga cggcggcctc ggcgcgcgcc tggtctacat cgaccggcag tacccgggca
     2521 gcatcgactg gacccggccc gacccgccgc agcgcgacgc caaccagggc cgcggcgagc
     2581 agttccgcgg cagcctgctg tggacgccga ccgatgcggc gcggctgcag ttcacttacc
     2641 ttgagcagga ctactccgcc gccaacgaca tcatcgtcgc cgacggcccg tacggcccgc
     2701 gctccagctc cgcgtcgctg cagccctggc ccaaccagca ccgcttcgcg ctgtaccagc
     2761 tgggcgggca atacgacttc gaacacgtgc gcatcgtctc gtccacctcg cgcacggaga
     2821 agaagcgcta caacagcatc gagagcctgg gcgcgctgct cggctcgccg ccaggcggca
     2881 cgccggcagc actggcgctg ccgttcatca cccaccagga ctccacctcg ttctcgcagg
     2941 aactgcgcgt gcagtcgatc gacacgccgc tggagtggct ggtgggcggc tactactacc
     3001 acgcgccgat caactacgag ctggtgctca acgtgcaggc gctgcaggac cttggcgggc
     3061 aggccaacaa cctggcgccg ctgctggcgc agctgcgcca ggcctacggc gcgctgtacg
     3121 gcgccaacaa cgagttgaac acgctgctgg gccagcttga ttcggtgacc ggcaatcccg
     3181 gctgcgaact gtccgtgctg tgcgccgaga cgcacgccaa ggcgaccgaa aaggcgctgt
     3241 ttttcgacct gtcgcgcaag ttctggcagc gtctcgaact ggaagccggt gcacgcctgt
     3301 accgcaccga ggtaaatggc ggcttcgtcg gcaccggcgc cggcgcgcgc ctggtcaaca
     3361 acggcatgtc gccggtgtac gtgctcaatg acctggtgga gcagggcatc aatcccaagt
     3421 tcaccgcgac gcttcatttc accagggaca tctccactta cttccaggcg gcgcgcggct
     3481 tccgcttcgg cggcatccag cagatcccgg cggacaaggt gcagaacgtg ccaggcacgt
     3541 tcaaatccga ctcgctgtgg aactacgagc tcggcctgcg caccgcgtgg ctgggcaaca
     3601 cgctgcacgc cgacgtcacc ggcttccaca tcgactacaa gaatcctcag gtgcagctga
     3661 aggacctgga agagctgaac tactacgaca acgtcggcgc ggcggtgagc aatggcgtgg
     3721 aaggcagcct ggaatggctg acgccactga ccggcattgc gctgtcagcc accggcggct
     3781 acaccaacgc gcacaccacc gagccattca aggcaggcaa cacgccggtg cccgccggcg
     3841 tgccgctacc cggatcggcc aaggtgcagt acagcgggca gctcacctat ctatggccgt
     3901 acggcggcga ctacagctgg ggcggcgcgc tgtcgtacgt ttatgtcggc aagacttcgc
     3961 ccggcctgca ggtgagccag tacgtctacg atttcggcgt ggtgaatttc agcagccagt
     4021 tcggcgcgcc gggctggcct ggcaagccga gcctggcgct gtcgatcgaa aatcttgcgg
     4081 accgcaccgc gccggtcagc atcatcggtt ccgccacggg atcaagctac tacctgctga
     4141 cgcctccgcg cacctacacc gcgcggctga cggtggaatt cgactgagcg cggcgcgcta
     4201 ttcgaattcg ctggcgtcgc gcgcgcagta ggccatggtg atggcgccgc cgccgacgtt
     4261 gaccgcaccg gccggcgcca tcatcgacag catcagctcc acgccttttt ccgtggcggc
     4321 gcgcgccagt tcgccgtagc ccggcatctt cggcacggcc agcggatcgc cgccgtagct
     4381 gatgttgacc atcggcacca gcaggccgcg gcggatctgc gcggcgccgt gcaggaacag
     4441 tttttccacc gccttgtcgt ggtggcgcac cttggccacc ggcgaggtct cgccgcggtt
     4501 ggcgcggatg atcggcttaa tgtcgagcgc attgccgatg atgtacgcgg cccagcccac
     4561 gctgtggtcg ccgcggcgcg cagtgcgcgc gtggatgtag tagaggtcgt ccggcacgat
     4621 gtaggcgtag gtgtgcggga tcaggtcgtc gagccgcttg cggatgtcca ctggcgaagc
     4681 gccttcgcgc gccatgcgca ctgcttccca ggccagcacg ccggggccgg tgaacagcga
     4741 ttcggaatcc accacgcgca gcgcgaacgg gccgggcacg ctcttggcgt tgcgcacgct
     4801 gtcgtagctc tgcaggatgg tgaaggacgc cttgtgcgcg ttatcgaagg tgagcgagcg
     4861 cgtggccatc agcgtgatca ggaacacgta gtcgtattcc agcaccaggc gcttgaggaa
     4921 caggtcttcg atctccttga ccgagaacgg cgtggtctcg gcgtcgatgc ccttgtcgcc
     4981 cagatggtcg cggtagaaca gcgcgccttt ttccgggtcg cggtcgtaca ccacgctgtg
     5041 gccgttcatg tgcaccgtcg acggcagcac atggatgtgg ttctgcacca cgaactcgtg
     5101 cgagacgtcg gtgctgacat cgatggcgat gccgactcgc atggtggaaa tgggggcgac
     5161 tccgggaagc gtgctgggcc gctattgtcg gcgcagcgcg ctgcgctggg tagccgcgtt
     5221 ttctgacgtg gatgtcagac gaagcgcggc gccgggcgat aaaaatgtct gaccgcacct
     5281 gcctgttgcg tgttctaaat tatatgacat atttataatc atataataat aaataacgag
     5341 ttagaaggca ttgcggtgac ggcgattttg cctcagatgg cgcggctcgc cggcgccgac
     5401 aggatcaccg gcccggtcgg caggccggta ggcgaaccgc ccgtcggctc cacgctcacc
     5461 gccagcgtca gctcgccttc gacctgcatc gccgcgggca aggccatttc cgcctggccc
     5521 tgcaagggca gcaggcccag aggccgcggg ccggattttt ccagcgccca cagttccagc
     5581 gcgtgctgcg catccaccgg gtagctgccg ctggccgcga ccttcatcgt ctttttctcg
     5641 ggatacatcg acaccaccca gcgcgcgtcc gacttgtccg gcttgagcac ggcgacgtaa
     5701 ggcatcagcg gcgcttcgtg gacgatcttg ggcatgacca cgatctgcat ggagaactgc
     5761 atctgctgct gcagggccac ggccagcagc agtgccgcgg cagtcgcaac ggccgaccag
     5821 gtgcgccaga agttgatgct gggcagcttg cgcggcggca acgtcggggt gttcatggca
     5881 ttgatcctcc tttcgatgcc gctccacacg atcccgcgcg gcgggaccgg cgtgatggcc
     5941 agcgccagcg gggcgagttt gcgttcccag gcgtgcacct cgcccagcag cgcggcgtcg
     6001 gcgcgcagca ggcggtcgaa gcgcatgcgt gcgcgcccct gcagggtgcc gagcacatat
     6061 tcggcggcca gcatgcggcg cagctgtggc tcgcggtagt tcatgcgtcg agacactcgc
     6121 gcagccgcgc cagcccacgc cgcacccagc tcttcaccgt gcccagcggc gccttcatgc
     6181 gcgccgccag ttcgtgatgc gtgtagccct cgtagtaggc cagcagcacg ctgctgcgct
     6241 gctccggctg caggccgcgc aggcagtcgt tgagccggcc cacgccttcg ccctcgatgg
     6301 cgccagcctc ggggctctgg ccgtcatcgg ccagcaccag cggcggcgcc tcgctctcct
     6361 cgggcaattc cacctccggc cgcttgacgc gcaacaggtc gagcgcgcgg tagcgcgcca
     6421 ccgtcgacag ccaggccagc ggcacgccct tggccggttc gtaagtctcg gccttctgcc
     6481 agatcttgag gtaacagtcc tgcaaggcgt cctcggccca gtcgcgtcgt ttcaacatac
     6541 gcagcagcag gccgtacaga tgcgcggagg ttgccgcgta gagttcacgg aacgcccgct
     6601 ggtcgccggc cgcggttgcg gcaagcagct gggctagagg ctggacgtcg tccatgttgt
     6661 ggatcttgtg tggcgcaatc ccggtgcggc gcggcgatca tactccggcc gccgtcggcg
     6721 catgcgcgca ccttataatt gcggcccagt
//
LOCUS       DASZZC010000590         5840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_870851,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000590 DASZZC010000000
VERSION     DASZZC010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870851"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VHE37_12605"
     CDS             complement(<1..548)
                     /locus_tag="VHE37_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HVT36419.1"
                     /translation="MTAKLKLALAQLNLWVGDVEGNVGKIIDAAAKARDELGASLVLC
                     PELSLIGYPPDDLLQRSAMPAVIEAGVERLLREVRGITLIAGLPEYEAGSIYNAAYVI
                     EDGQVIARARKQLLPNYGVFDERRHFRPGKGTLTFEHRGVRVGLCICEDIWGPNPAAQ
                     AQAAGAQLLLNINASPFDAGKS"
     gene            complement(583..1458)
                     /gene="sucD"
                     /locus_tag="VHE37_12610"
     CDS             complement(583..1458)
                     /gene="sucD"
                     /locus_tag="VHE37_12610"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HVT36420.1"
                     /translation="MSILINKNTKVICQGFTGKQGTFHSEQAIAYGTKMVGGVSPGKG
                     GGTHLNLPVFNTAHDAVKATGADATMIYVPAPGAADAVLEAADAGIKIIICITEGIPV
                     IDMIKVKATLRAQYPGSLLVGPTCPGVITPGECKIGIMPGHIHKPGKIGVVSRSGTLT
                     YETVHQTTQNGLGQSTCVGIGGDPVRGMGFIDVIAMFEKDPATEGIIMVGEIGGSSEE
                     EAAAYIKKSVKKPVVAYIAGVTAPAGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTV
                     RSPADLGSAMKGLLK"
     gene            complement(1462..2628)
                     /gene="sucC"
                     /locus_tag="VHE37_12615"
     CDS             complement(1462..2628)
                     /gene="sucC"
                     /locus_tag="VHE37_12615"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HVT36421.1"
                     /translation="MNVHEYQAKEIFTRYGIPVPQGQLAASPEAARAAAKQLGTEKVV
                     VKAQVHAGARGKAGGVKLVNGPDAAEDAAKAMLGKHLVTIQTDAKGLPVNSVWVEKPS
                     NIARELYLSALVDRTQEKIVFMASPDGGMDIEKVAHDTPERIKHVFVSPSAGLQPYQA
                     RELAFFMGLTKDQADQFTRVLTGLYRIFTECDASLVEINPLIVTAEGNVMALDGKLNF
                     DDNALFRQKEIAAMRDPSQEDEVERAAAKYDLNYVTLEGNIGCMVNGAGLAMATMDIV
                     KLHGGQPANFLDVGGGTTAEKVTEAFKLITSSPSVKAIFINIFGGIVRCDLIAEGIIQ
                     ACKQTGLKIPVVARLQGTNMEKGQALLRESGLAITPVSDLTEAAQTVVRLAQGK"
     gene            2887..3087
                     /locus_tag="VHE37_12620"
     CDS             2887..3087
                     /locus_tag="VHE37_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36422.1"
                     /translation="MSLFKLLLIGAAIWIALRYLRRAGEAAPPRAKGDDYIVMARCAQ
                     CGVHLPSSSLTADGRCGKCSDG"
     gene            3080..4678
                     /locus_tag="VHE37_12625"
     CDS             3080..4678
                     /locus_tag="VHE37_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT36423.1"
                     /translation="MADHGVTTAAAPAQTWEDWRALRFLSLVRLLLIALLALLYHKGY
                     GADLLQQLSRREFLWSCAVYACAGLLMFVPVIYRTPRLGIQVHLHFVVDLAGIGLLVY
                     FSGGVPDGLGIILIYPLLSCALLLSTRMAVVHAAGATLAMFGEEIIRQSQMGFSASEF
                     TSTGLLGVMFFATSAAASAVAARARTSEALAERVGSEFADLSRLNESILESMHTGVVV
                     VDAAREVRTINAAAVRLLGAGARAGRKLGDVALPLDRTLDAWLRGYAARAEPVATARG
                     APEVVPRFARLGWNTNPPILILLDDAAALRERAQQMKLAALGRLSASIAHEIRNPLAA
                     ISHAGQLLAEAPEQPPENMRLLSMIQRHSGRIDKIVKDVLLLSKREAAEPAQLPLRDW
                     LLRTVAMYQEGFPEQPRPIELLEVAPELVVTFDPSHLQQVVFNLWDNSFEHGGPGAMV
                     LMHAARDEASGQLYLECADNGPGIKPELLERIFEPFFTTSSQGTGLGLYLSRELCEYN
                     QARLTLAPQDKGAAFRIYFPHSNA"
     gene            4675..>5840
                     /locus_tag="VHE37_12630"
     CDS             4675..>5840
                     /locus_tag="VHE37_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT36424.1"
                     /translation="MSKPTAATALIVDDEADIRELVEITLSRMGLKTASAATLAEART
                     LLAEREFDLCITDMRLPDGNGISLVGEIAREHPQMPVAMITAYGNAQAAVESLKAGAF
                     DFVSKPVDIAVLRKLVDTALKLKRGAAPPKPGDGGLLGDHPSIEQLRGMIERLARSQA
                     PVHIAGESGTGKELVARLIHARGPRAAGPFVPVNCGAIPTELMESEFFGHVRGSFTGA
                     VKDKAGLFVAAEGGTLFLDEVADLPLHMQVKLLRALQERSVRPVGAENEVPVNVRIIS
                     ATHKNLAELVSAGAFRQDLYYRLNVIEVRTPALRDRAQDIPLLARHVLERLARGTGMS
                     AVPQLEKSALDQLAIYPFPGNVRELENILERAITLCDGARITAADLQLRQNSEVP"
BASE COUNT          882 a         1993 c         1952 g         1013 t
ORIGIN      
        1 tcactcttgc cggcgtcgaa cggcgaggcg ttgatgttga gcagcagctg cgcgccggcg
       61 gcctgcgcct gcgccgccgg gttcggcccc cagatgtcct cgcagatgca caggcccacg
      121 cgcacgccgc gatgctcgaa ggtcagcgtg cccttgcccg gcctgaaatg gcggcgctcg
      181 tcgaacacgc cgtagttggg cagcagctgc ttgcgcgcgc gcgcaatgac ctggccgtcc
      241 tcgatcacgt aggccgcgtt gtagatgctg ccggcctcgt actcgggcag gccggcgatg
      301 agcgtgatgc cgcgaacttc ccgcagcagc cgctcgacgc ccgcttcgat caccgccggc
      361 atggcgctgc gctgcaggag atcgtccggc ggatagccga tcagcgacag ctccgggcac
      421 agcaccaggc tggcgccgag ttcgtcgcgc gccttcgccg ccgcgtcgat gatcttgccg
      481 acattgccct cgacatcgcc gacccacagg ttgagctggg ccagggcgag cttgagtttc
      541 gcggtcattt gattgtgcag acccgcacgg ccctgcgtcg ggttacttga gcagcccctt
      601 catcgcgctg cccaggtccg ccggcgaacg caccgtcttc acgcccgccg cttccagcgc
      661 cgcgaatttt tcatcggccg tgcccttgcc gcccgcgatg atcgcgccgg cgtggcccat
      721 gcgcttgccc gcgggcgcgg tgacgccggc gatgtaagcc acgacaggct tcttcacgct
      781 cttcttgatg tacgcggccg cttcttcttc ggacgagccg ccgatttcgc cgaccatgat
      841 gatgccctcg gtcgcggggt ctttctcgaa catggcgatg acgtcgatga agcccatgcc
      901 gcgcaccggg tcgccgccga tgcccacgca ggtgctctgg cccaggccgt tctgcgtggt
      961 ctggtgcacg gtttcgtagg tcagcgtgcc gctgcgcgag accacgccga tcttgcccgg
     1021 cttgtggatg tggcccggca tgatgccgat cttgcattcg cccggcgtga tcacgccggg
     1081 gcaggtgggg cccaccagca ggctgcccgg gtactgcgcg cgcagggtgg ccttgacctt
     1141 gatcatgtcg atcaccggga tgccttcggt gatgcagatg atgatcttga tgccggcatc
     1201 cgccgcctcg agcaccgcgt cggcagcgcc gggagcaggc acgtagatca tggtggcatc
     1261 ggcaccggtc gccttgaccg cgtcgtgcgc ggtgttgaac accggcaggt tcaggtgcgt
     1321 gccgccgccc ttgccgggcg acacgccgcc gaccatcttg gtgccgtagg caatcgcctg
     1381 ttcggaatgg aacgtgccct gcttgccggt gaagccctgg cagatgactt tggtgttctt
     1441 gttgatcagg atcgacatgg cttacttccc ctgcgccagg cgcacgaccg tctgcgcggc
     1501 ttcggtgaga tcggaaacgg gggtgatggc aaggccggac tcgcgcagca gcgcctggcc
     1561 cttctccatg ttggtgccct gcaggcgcgc caccaccggg atcttcaggc cggtctgctt
     1621 gcacgcctgg atgatgccct cggcgatcag gtcgcagcgc acaatgccgc cgaagatgtt
     1681 gatgaagatg gccttgaccg acgggctcga ggtgatgagc ttgaatgctt ccgtcacctt
     1741 ctcggcggtg gtgccgccgc cgacgtcgag gaagttggct ggctggccgc cgtgcagttt
     1801 cacgatgtcc atcgtcgcca tggccaggcc cgcgccgttg accatgcagc cgatgttgcc
     1861 ttcgagcgta acgtagttga ggtcgtactt cgccgccgca cgttccactt cgtcttcctg
     1921 cgaaggatcg cgcatcgccg cgatctcctt ctggcggaac agcgcgttgt cgtcgaagtt
     1981 gagcttgccg tcgagcgcca tcacgttgcc ttcggcggtg acgatcagcg ggttgatctc
     2041 caccaggctc gcatcgcact cggtgaagat gcggtaaagg ccggtgagca ccctggtgaa
     2101 ctggtccgcc tggtccttgg tcaggcccat gaagaaggcc agctcgcgcg cctggtacgg
     2161 ctgcagcccg gcggacggcg agacgaacac gtgcttgatg cgctcgggcg tgtcatgcgc
     2221 caccttctcg atgtccatgc cgccatcggg cgaggccatg aacacgatct tctcctgcgt
     2281 gcggtcgacc agcgcggaca gataaagctc gcgcgcgatg ttcgacggct tctccaccca
     2341 caccgagttg accggcaggc ccttggcgtc cgtctggatg gtgaccaggt gtttgccgag
     2401 catggccttg gcggcgtctt ccgccgcatc ggggccgtta accagcttga cgccgccggc
     2461 cttgccgcgc gcgcccgcat ggacctgcgc cttgaccacc accttttccg tgcccagctg
     2521 cttggcggcg gcacgcgccg cttcgggact cgctgccagc tggccctgcg gcacgggaat
     2581 gccgtagcgc gtgaagattt ccttggcctg atactcgtgt acgttcatgc ggtccctgtc
     2641 agcggagtaa gggggcgcga ttgtgtccga attgccccgg cggagccaac cgcatgccat
     2701 ttctgcaagt tataaaatat ttattgaaaa taaaattaat gtatttttta taacagtgaa
     2761 aacggccatg cggtgacacg taaaatcggc tgtatacatg gtgcatgcag cggcgcccgt
     2821 ggcgcaccgt ccgtttgcgc gagctgcgcc ggcagcctca gcgcatttcc tccacactga
     2881 tcgccaatga gcctgttcaa actgctgctg atcggcgcgg ccatctggat cgcgctgcgc
     2941 tacctgcgac gcgccggcga agcggcgccg ccgcgcgcca agggcgacga ctacatcgtg
     3001 atggcgcgct gcgcgcaatg cggcgtgcac ctgccgtcct cctcgctcac ggccgatggc
     3061 cgctgcggca agtgcagcga tggctgatca cggcgtgacc accgccgccg cgccggcgca
     3121 gacctgggaa gactggcgcg ccctgcgctt cctgtcgctg gtgcggctgc tgctgatcgc
     3181 actgctggcg ctgctgtacc acaagggcta cggcgccgac ctgctgcagc agctgtcgcg
     3241 gcgcgaattc ctgtggagct gcgcggtcta cgcctgcgcc ggcctgctga tgttcgtgcc
     3301 ggtgatctac cgcacgccgc ggctgggcat ccaggtgcac ctgcactttg tagtcgatct
     3361 ggccggcatc ggcctgctgg tgtatttctc cggcggcgtg cccgacggcc tgggcatcat
     3421 cctgatctac ccgctgctgt cgtgcgcgct gctgctgtcc acgcgcatgg cggtggtgca
     3481 cgccgccggc gccacgctgg ccatgttcgg cgaggagatc atccgccagt cgcagatggg
     3541 tttttccgcc agcgagttca cctccactgg cctgctcggc gtgatgttct ttgccaccag
     3601 cgcggctgcc agcgcggtgg cggcacgcgc gcgcaccagc gaggcgctgg ccgagcgcgt
     3661 gggcagcgag ttcgccgacc tgtcgcggct caacgagagc atcctcgaat cgatgcacac
     3721 cggcgtggtg gtggtggacg ccgcgcgcga agtgcgcacc atcaacgccg ccgccgtgcg
     3781 cctgctcggc gccggcgcgc gcgccgggcg caagctgggg gacgtcgcac tgccgctgga
     3841 ccgcacgctc gacgcctggc tgcgcggcta cgcggcgcgt gccgaaccgg tggccaccgc
     3901 acgcggcgcg cccgaagtgg tgccgcgctt cgcccgcctg ggctggaaca ccaacccgcc
     3961 gatcctgatc ctgctcgacg acgccgccgc gctgcgcgag cgcgcgcagc agatgaagct
     4021 ggccgcgctg ggccggctgt cggcgtcgat cgcgcacgag atcaggaatc cgctggccgc
     4081 catcagccac gccggccagc tgctggccga agcccccgaa cagccgccgg aaaacatgcg
     4141 cctgctgtcg atgatccagc gccattccgg gcgcatcgac aagatcgtca aggacgtgct
     4201 gctgctgtcc aagcgcgagg cggccgagcc ggcgcagctg ccgctgcgcg actggctgct
     4261 gcgcaccgtg gcgatgtacc aggaaggttt ccccgagcag ccgcggccga tcgaactgct
     4321 ggaagtggcg ccggagctgg tggtgacgtt cgatccctcg cacctgcagc aggtggtgtt
     4381 caacctgtgg gacaacagct tcgagcacgg cgggccgggc gccatggtgc tgatgcacgc
     4441 ggcgcgcgac gaggcgtcgg gccagctcta cctggaatgc gccgacaacg ggccgggcat
     4501 caagccggaa ctgctcgagc gcatcttcga gccgttcttc accaccagct cgcagggcac
     4561 tggcctgggc ctttatctgt cgcgcgagct gtgcgaatac aatcaggcgc gcctgaccct
     4621 ggcgccgcag gacaagggcg ccgccttccg catctatttc ccgcacagca acgcatgagc
     4681 aagcccaccg ccgccactgc cctgatcgtc gacgacgagg ccgacatccg cgagctggtg
     4741 gagatcacgc tgtcgcgcat gggcctgaag accgcatcag ccgccacgct ggccgaggcg
     4801 cgcacgctgc tggccgagcg cgagttcgac ctgtgcatca ccgacatgcg cctgcccgac
     4861 ggcaacggca tcagcctggt gggcgagatc gcgcgcgagc acccgcagat gccggtggcc
     4921 atgatcacgg cgtacggcaa tgcgcaggcg gcggtggaaa gcctcaaggc cggcgcgttc
     4981 gacttcgtct ccaagccggt ggacatcgcc gtgctgcgca agctggtgga caccgcgctc
     5041 aagctcaagc gcggcgcggc gccgcccaaa ccgggcgacg gcggactgct cggcgaccat
     5101 cccagcatcg aacagctgcg cgggatgatc gagcggctgg cgcgctcgca ggcgccggtg
     5161 cacatcgccg gcgaatcggg caccggcaag gagctggtgg cgcggctgat ccacgcgcgc
     5221 ggcccgcgtg ctgcgggtcc atttgtgccg gtgaactgcg gcgccattcc caccgaactg
     5281 atggaaagcg agttcttcgg ccacgtgcgc ggcagcttca ccggcgcggt caaggacaag
     5341 gccggcctgt tcgtggcggc cgagggcggc acgctgttcc tcgatgaagt cgccgacctg
     5401 ccgctgcaca tgcaggtgaa gctgctgcgc gcgctgcagg agcgcagcgt gcggccggtg
     5461 ggcgcggaga acgaggtgcc ggtgaacgtg cgcatcatct cggctacgca caagaacctg
     5521 gccgaactgg tcagcgccgg cgccttccgc caggatctct actaccgcct caacgtgatc
     5581 gaggtgcgca cgccggcgtt gcgcgaccgt gcgcaggaca tcccgctgct ggcgcgccac
     5641 gtgctcgaac ggctggcgcg cggcaccggc atgagcgcgg tgccgcagct ggaaaaaagc
     5701 gcgctcgacc agctggccat ttacccgttc cccggcaatg tccgcgagct ggaaaacatc
     5761 ctggaacgcg ccatcaccct gtgcgacggc gcccgcatca ccgctgccga tctccagctg
     5821 cgccagaaca gcgaggtgcc
//
LOCUS       DASZZC010000591         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_743411,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000591 DASZZC010000000
VERSION     DASZZC010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743411"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VHE37_12635"
     CDS             <1..843
                     /locus_tag="VHE37_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36425.1"
                     /translation="GRRLAWREYGERTGRPCIYTAGTPASGLCGAVFHEAALRAGVRW
                     VAPDKPGSGHSDFMPRRRLLDWADDVAQLADHLRLTRFAVAGESGGGPHTLALAARLP
                     QRVTVAVCVAGMGQGDSPELRRAMTLQFRTMLWLAAHAPWLPGFLMGRMVAAVADPQR
                     RAALLQRQMKLAPAADAAAFARHPEKLEQVLLAYVDAFRGGTRGPVQEFALFTRPWEF
                     EPAAIEAPVHLWHGEEDRNVPVSAARRLAAMIPGCTAHYITGAGHGLWDQIDAIVQTV
                     ATAA"
     gene            complement(869..1147)
                     /locus_tag="VHE37_12640"
     CDS             complement(869..1147)
                     /locus_tag="VHE37_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36426.1"
                     /translation="MGNTRIIVHLPAHRRAALNIERDLDAPRTYDRYIGEYIEFLRKE
                     GIHAGFDLTTDERDDDKGIFTIDEMNHEAKRKAHAWLETQPDIWNWIP"
     gene            1380..3035
                     /locus_tag="VHE37_12645"
     CDS             1380..3035
                     /locus_tag="VHE37_12645"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HVT36427.1"
                     /translation="MRYHFVALFGAVLIGGAAASTASTIVPPSKLITADLLRGHVRFI
                     SSDLTEGRGTASRGDDIAMAYIQAQMESMGLQPGAPDGSWYQRFDVVGINSHIAPTVA
                     FSHGGQSLVLNSGAEFIGVAGEQKSDLAISNAEVVFAGYGIQAPEYRWDDFKDVDVRG
                     KILLVMNNDPEDDPSLFAGRTRLYYGRWMYKYEQAARMGAAGAIIIHTEHSAAYPWQV
                     VQTSWSGENFELPQEHEPRTQLKGWATEDASRRIAALGGFDLDQLRAQAEKREFRPVP
                     LGVTLSLAIHSQVKRQQTANVIGRLPGHDPQLAREALFYTAHHDHLGIKQGAAPGEDA
                     IYNGALDNASGCATLLAIARAMASGPKLKRTVYFASVGAEEQGLLGSRYLAEHPPLPA
                     GRIAADINMDGISIWGRTHDVTVIGLGKSTLDDDIRAVAAAQKRTVVGDPFPDKGSYY
                     RSDQFSFAHIGVPGAYLGGGIEVLGRPAGWGAAQKEAFVKNDYHQPSDELRDSWDFSG
                     AVEDAQLLYQLGARVADAPQLPAWTPGDEFEGARKRAIGQAQP"
     gene            3220..>4313
                     /locus_tag="VHE37_12650"
     CDS             3220..>4313
                     /locus_tag="VHE37_12650"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HVT36428.1"
                     /translation="MEAIRFEHTRGKHRARWPRAFFAAAIGAALTLSACGGGDDNPTI
                     TGGGGGGTTTGKAAIAMGDAAGDFYSYQVTLTSLKLKPASGAEVETLPAPVLIDFTQL
                     VGVSELMSMAGVPTGSYTGAHVVLDFSGANIIQPNASGAPTTLSPVDASGNPLTTLDF
                     TVNFPAPVSITEGNTSSLSLDFDVPSSSIVEGSNVIVLPIMTASSTPDTARTERVQGQ
                     LASVSGSGASRTLTVNVQPLNHSGGDFGSVAVKTDANTVYDVNGSIKTGSAGATALAA
                     LTMPTPVLTSNDVAADGSLTAKLVRAGSSLGAANADEANGVVLARNGNTVTVLGAVRN
                     NGTAARHFANNLQVDLTGADVAGGAVTDAS"
BASE COUNT          710 a         1441 c         1470 g          692 t
ORIGIN      
        1 ggccgccgcc tggcgtggcg cgagtacggc gagcgcacgg gccgcccgtg catctacacc
       61 gccggcacgc cggcctcggg cctgtgcggc gcggtgttcc acgaggccgc gctgcgcgcc
      121 ggcgtgcgct gggttgcgcc agacaagccg ggcagcggcc actccgattt catgccgcgg
      181 cgccggctgc tcgactgggc cgacgatgtg gcgcaactgg ctgaccacct gcgcctgacg
      241 cgctttgccg tggccggcga gtccggcggc ggcccgcaca cgctggcgct ggcggcgcgc
      301 ctgccgcagc gcgtgaccgt ggccgtctgc gttgccggca tggggcaggg cgactcgccg
      361 gaactgcgcc gcgcgatgac gctgcagttc cgcaccatgc tgtggctggc ggcgcacgct
      421 ccatggctgc ccggattcct gatgggccgc atggtggccg cggtggccga tccgcagcgg
      481 cgcgcagcgt tgctgcagcg gcagatgaaa ttggcgcccg ccgccgatgc cgcggccttt
      541 gcgcgccacc cggaaaaact cgaacaggtg ttgctggcct atgtcgatgc gttccgtggc
      601 ggcacgcgcg ggccggtgca ggagttcgcg ctgttcacgc gcccgtggga attcgagccc
      661 gccgccatcg aggcgccggt gcacctgtgg catggagaag aggaccgcaa cgtgcccgtt
      721 tccgcggcac ggcggctggc cgccatgatc cccggctgca ccgctcacta catcaccggc
      781 gccgggcacg gcctgtggga ccagatcgac gccatcgtgc agacggtggc gaccgccgcg
      841 taatcacgcg tggacggcgc tcagcgcgtc acggaatcca gttccagata tcgggctgcg
      901 tctccagcca ggcgtgcgcc ttgcgcttgg cctcgtggtt catctcgtcg atggtgaaga
      961 tgcccttgtc gtcgtcgcgc tcgtcggtgg tgaggtcgaa gccggcgtgg atgccttcct
     1021 tgcgcaggaa ctcgatgtat tcgccgatgt agcggtcgta ggtgcgcggg gcgtcgagat
     1081 cgcgctcgat gttcagcgcg gcgcggcggt gtgccggcag gtggacgatg atgcgggtgt
     1141 tgcccatgtc tggctcctct tgttggtggt cggacctgcg gttcaaattg cgtctcgcgg
     1201 cgccgcgcaa gtgtcgcgca aagcgaaaaa actgcctacc gcaccgctcc cggcgatgtt
     1261 atgaaaaata acatataaat aaaataataa aataaattat aacatcgaaa atgcaggtgc
     1321 ggtggaggcc tgcgcggacg tttatagtgc ccgcaacttt catccatgga ctaagaccca
     1381 tgcgctacca cttcgtcgcc ctgttcggcg cggtgctcat cggcggcgct gccgcgagca
     1441 cggccagcac catcgtgccg ccgtcgaagc tgatcaccgc cgacctgctg cgcggacatg
     1501 tgcgcttcat ctcgtcggat ctcaccgagg gccgcggcac cgcctcgcgc ggcgacgaca
     1561 tcgccatggc ctacatccag gcgcagatgg aaagcatggg cctgcagcct ggcgcacccg
     1621 acggcagctg gtaccagcgc ttcgacgtgg tgggcatcaa cagccacatc gcgcctacgg
     1681 tggcgttcag ccacggcggg cagagcctgg tgctcaacag cggcgccgag ttcatcggcg
     1741 tggccggcga gcagaagagc gacctcgcca tcagcaacgc cgaggtggtg ttcgccggct
     1801 acggcatcca ggcgccggaa taccgctggg acgatttcaa ggatgtggac gtgcgcggca
     1861 agatcctgct ggtgatgaac aacgacccgg aggacgaccc cagcctgttc gccggcagga
     1921 cgcgcctgta ctacgggcgc tggatgtaca agtacgagca ggcggcgcgc atgggcgcgg
     1981 ccggcgccat catcatccac accgagcact cggcggcgta cccgtggcag gtggtgcaga
     2041 cctcgtggag cggcgagaac ttcgagctgc cgcaggagca cgagccgcgc acgcagctca
     2101 agggctgggc cacggaagac gcttcgcggc gcatcgccgc gctcggcggg ttcgatctcg
     2161 accagctgcg cgcgcaggcg gagaaacgcg aattccgccc ggtgccgctg ggcgtcacgc
     2221 tgtcgctggc catccacagc caggtcaagc gccagcagac cgccaatgtc atcggcaggc
     2281 tgccggggca cgatccgcag ctggcgcggg aagcgctgtt ctacaccgcg catcacgatc
     2341 acctgggcat caagcagggc gcggcgccgg gcgaagacgc catctacaac ggcgcgctcg
     2401 acaacgcgtc cggctgcgcc acgctgctgg ccatcgctcg cgccatggcg tcaggcccca
     2461 agctcaagcg cacggtgtac ttcgcctcgg tgggcgcgga agagcagggg ctgcttggct
     2521 ccaggtacct ggcggagcat ccgccgctgc cggccgggcg catcgccgcc gacatcaaca
     2581 tggacggcat cagcatctgg gggcgcacgc acgacgtcac cgtgatcggc ctgggcaagt
     2641 ccacgctcga cgacgacatc cgcgccgtcg ccgccgcgca gaagcgcacg gtggtcggcg
     2701 atccgttccc ggacaagggc agctattacc gctccgacca gttctccttc gcgcacatcg
     2761 gcgtgccggg cgcgtacctg ggcggcggca tcgaggtgct cggcagaccc gccggctggg
     2821 gcgcggcgca gaaggaagcg ttcgtgaaga acgactatca ccagccgtcc gacgagctgc
     2881 gcgacagctg ggatttttcc ggcgcggtgg aagacgcgca gctgctgtac cagctgggcg
     2941 cacgcgtggc cgatgcgccg cagctgccgg cctggacgcc gggcgacgaa ttcgagggcg
     3001 cgcgcaagcg cgccatcggc caggcgcaac cctgatgtga agttttttcc gcgcactttt
     3061 ttcgtcgcgt aaaaaatttt catgctacgg aattggtagc aagaaagttc gcaatttatc
     3121 tcgcatgcgt tttctgaact gcgcctgaat tgcggaagtc tctcctgcga gcacttctcc
     3181 cgtcgcgccg cggcggggct gctctttgga ggatgaatca tggaagcgat ccgcttcgag
     3241 cacactcgtg gaaaacacag ggcacgctgg ccgcgtgcct tttttgcggc cgccattggc
     3301 gccgcgctga cgctgtccgc ctgcggcggc ggcgatgaca accccaccat caccggtggc
     3361 ggcggtggcg gcaccaccac cggcaaggca gccatcgcga tgggcgatgc ggccggcgat
     3421 ttctacagct accaggtcac cctgacttcg ctcaagctga agccggcttc cggtgccgag
     3481 gtggaaacct tgccggcgcc ggtgctgatc gacttcaccc agctggtggg tgtctccgag
     3541 ctgatgtcca tggccggcgt gcccaccggc agctacaccg gtgcgcacgt ggtgctggat
     3601 ttcagcggcg cgaacatcat ccagccgaat gcgtccggcg cgccgaccac gctcagcccg
     3661 gtcgatgctt cgggcaatcc gctgaccacg ctggacttca ccgtcaactt ccctgcgccg
     3721 gtaagcatta ccgagggcaa cacctcgtcg ctgtcgctgg actttgacgt gccgtcatcg
     3781 agcatcgtcg agggcagcaa tgtcatcgtg ctgccgatca tgaccgcgtc gagcacgccc
     3841 gataccgcgc gcaccgagcg cgtgcagggg cagctggcct cggtcagcgg cagcggcgcc
     3901 agccgcacgc tgacggtgaa cgtgcagccg ctcaatcact cgggtggcga tttcggcagc
     3961 gtggcggtca agaccgatgc caacacggtc tatgacgtca acggcagcat caagacgggc
     4021 agtgccggcg ccaccgcgct ggcggccctg accatgccga cgccggtgct gaccagcaac
     4081 gatgtcgcgg cggatggttc gctcaccgcc aagctggtgc gtgccggcag cagcctgggc
     4141 gcggccaatg ccgacgaagc gaacggcgtg gtgctggcgc gcaacggcaa taccgttacg
     4201 gtgctgggcg ccgtgcgcaa caacggcacc gccgcgcggc acttcgccaa caatctgcag
     4261 gtcgatctca ccggcgctga tgtcgccggc ggtgcggtga ccgacgccag caa
//
LOCUS       DASZZC010000592         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1015122,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000592 DASZZC010000000
VERSION     DASZZC010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015122"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VHE37_12655"
     CDS             complement(<1..385)
                     /locus_tag="VHE37_12655"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HVT36429.1"
                     /translation="MGQTKIPVIRNQALNERDYGDLSGLNKDDARKKWGEEQVHIWRR
                     SFDIAPPGGESLRDTAARVLPYYIQEILPRVMRGERVLVAAHGNSLRALVMVLDRHTK
                     ESILKLNIDTGVPMIYRLNADTTVAE"
     gene            481..642
                     /locus_tag="VHE37_12660"
     CDS             481..642
                     /locus_tag="VHE37_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36430.1"
                     /translation="MAAHGNSLRALVMVLDRHTKESILKLNIDTGVPMIYRLNADTTV
                     AEKRDLAAA"
     gene            639..1157
                     /locus_tag="VHE37_12665"
     CDS             639..1157
                     /locus_tag="VHE37_12665"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT36431.1"
                     /translation="MSKIDIIELDVTTAPAAINELASVLADCVNGGASVNFMLPYSAA
                     DAAEFFRKIVPSIASGETILLAARIDGRIVGTVQLGLDTPPNQPHRAEVKKMLVHRAA
                     RGKGVGAALLSEVEARAKQKGRTLLVLDTASDDARRLYERGGWQRLGNIPDYALLPDG
                     ALCDTTIYWTRQ"
     gene            complement(1211..>1571)
                     /locus_tag="VHE37_12670"
     CDS             complement(1211..>1571)
                     /locus_tag="VHE37_12670"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HVT36432.1"
                     /translation="AALALTPAVAFGQASTATGAVGGAVVGGVVGGPVGAVVGAGVGG
                     TVGMAAEPPAPVVTYVERESIPSVAVQEEVVVGQPLPATVTLHTVPKYKQYSYAVVNH
                     KRVIVEPRSRKVVKIIE"
BASE COUNT          291 a          523 c          485 g          272 t
ORIGIN      
        1 tttccgccac ggtggtgtca gcgttgagcc ggtagatcat tggcacgcca gtgtcgatgt
       61 tgagcttgag gatgctctcc ttcgtgtgac ggtcgagcac catcaccagc gcccgcaacg
      121 aattgccgtg cgcggccacc agcacacgct caccgcgcat cacccgcggc aggatctcct
      181 ggatgtaata cggcagcacg cgcgccgcag tgtcgcgcag gctttccccg cccggcggcg
      241 cgatgtcgaa ggagcgccgc cagatgtgga cctgctcctc accccatttc ttgcgggcgt
      301 catccttgtt gagaccggac aggtcgccgt aatcgcgctc gttgagcgcc tgattgcgga
      361 tcaccggaat tttcgtctgg cccatctcat cgagaatgat gtccagcgtg cgctgcgcgc
      421 gtgcttccgt actacattca ggaaatcctg ccgcgggtaa tgcgcggtga gcgcgttctg
      481 gtggccgcgc acggtaattc actgcgcgcg ctggtgatgg tgctcgaccg ccacaccaaa
      541 gagagcatcc tcaagctcaa catcgatacc ggcgtgccga tgatctaccg gctcaacgcc
      601 gacaccaccg tggcggaaaa gcgcgatctg gccgcggcgt gagcaagatc gatatcatcg
      661 aactcgacgt aacaacggcg cctgccgcca tcaatgaact ggccagcgtg ctggccgact
      721 gcgtcaacgg cggcgcgtcg gtgaatttca tgctgccata cagcgccgca gacgcagcgg
      781 aatttttcag gaaaatcgtt ccctcgatcg ctagcggcga gacgatcctg ctggcggcga
      841 gaatagatgg gcgcatcgtc ggcaccgtgc agctcggcct cgacaccccg ccgaaccagc
      901 cgcatcgcgc cgaggtgaag aaaatgctgg tgcaccgcgc cgcgcgcggc aaaggcgtgg
      961 gcgccgcgct gctgtcggaa gtcgaggcgc gggcgaagca aaaaggccgc accctgctgg
     1021 tgctcgatac ggcgagcgac gacgcgcgcc gtctttacga gcgcggcggc tggcagcggc
     1081 tcggcaatat tccggattat gcgctgctgc ccgatggcgc actttgcgac accacgatct
     1141 attggacacg ccagtaaaaa accccgccaa gatggacctt ggcggggctt ttagtttccg
     1201 cgttatcaaa ctattcgatg atcttcacga ccttgcgcga acgcggttcg acgatcacgc
     1261 gcttatggtt cacgaccgcg tagctgtact gtttgtactt cggcacggtg tgcagggtca
     1321 cagtcgccgg aagcggctgg ccgacgacca cctcttcctg aaccgcgacc gacggaatcg
     1381 attcacgctc cacataggtt acgaccggag ccggcggttc cgccgccatg ccgaccgtgc
     1441 cgccgacgcc ggcgccaacc acggcaccga ccgggccgcc aacgacgccg ccgaccaccg
     1501 cgccgccaac ggcgccggtc gccgtcgaag cctggccgaa cgccaccgct ggcgtcaggg
     1561 ccagggccgc t
//
LOCUS       DASZZC010000593         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_371827,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000593 DASZZC010000000
VERSION     DASZZC010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371827"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VHE37_12675"
     CDS             <1..359
                     /locus_tag="VHE37_12675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HVT36433.1"
                     /translation="DPQLAVTTLDSNGKKARFMRHAARALQLPNVEVVEARAEDHAGV
                     YDTIVSRAFASLADFFDKTAHLLAPSGAWVAMKGKLAAAELAGIPASVHIRETQRLRV
                     PGLDEERHAVIAEKKP"
     gene            356..1138
                     /locus_tag="VHE37_12680"
     CDS             356..1138
                     /locus_tag="VHE37_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HVT36434.1"
                     /translation="MTHVIAVANQKGGVGKTTTSINLAASLAATRRRVLLVDLDAQGN
                     ATMGAGVDKSALAHTACDVLLERCKIGAALQSLESGFAILPANSDMTEAEVKLRDLPV
                     GDFALKSALAEVSSQFDYVIVDCPPALSKLTVNALVAADGVLIPMQCEYYALEGLTAL
                     MDTINKIKRVINPKLEIEGLLRTMFDPRNNLANDVSAQLTQHFGDKVYRTIVPRNVRL
                     AEAPSHGMPVMQYDAQSSGAKAYLALAGEVLRRHDGGQLRLN"
     gene            1138..>1861
                     /locus_tag="VHE37_12685"
     CDS             1138..>1861
                     /locus_tag="VHE37_12685"
                     /inference="COORDINATES: protein motif:HMM:NF028434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HVT36435.1"
                     /translation="MNVLVPLPRRDFDPTEVAVSWKILSEAGHELIFATPDGSVAAAD
                     PVMISGEGLDVWGFIPGLKKLKLIGLALRAHAPARRAYASLQRDARFLAPKKYAELNA
                     ADFDALLLPGGHAKGMREYLEDKTLQSVVADFFDAAKPVAAVCHGVVLAARSLSKKTG
                     KPVLHGKRTTALTWKLEKSGWDLTRFFARFWDGDYYRTYGEQPGQPAGYMSVQQEVTR
                     ALALPSDFRDVPADAPHHFMKAS"
BASE COUNT          338 a          627 c          616 g          280 t
ORIGIN      
        1 ccgatccgca gctcgcggtg accacgctcg acagcaatgg caagaaagcg cgcttcatgc
       61 gccacgcggc gcgcgcgctg caactgccca acgtggaagt ggtcgaagcg cgcgccgaag
      121 atcatgccgg cgtgtacgac accattgtca gccgcgcctt cgcctcgctg gcagacttct
      181 tcgacaaaac cgcacacctg cttgcaccaa gtggcgcctg ggtggcgatg aagggcaaac
      241 tagccgccgc agaactcgcc ggcatcccgg cgagtgtcca tattcgggag acgcagcgcc
      301 tgcgcgtgcc gggcctcgac gaggagcggc atgcggtgat cgctgaaaag aaaccatgac
      361 ccatgtaatc gccgtcgcca accagaaggg cggtgtcggc aagaccacga cctcgatcaa
      421 cctcgccgct tcgctcgcgg cgacgcgccg ccgcgtgctg ctggtggacc tggacgcgca
      481 gggcaacgcc accatgggcg cgggcgtgga caagagcgcg ctggcgcaca cggcgtgcga
      541 cgtgctgctc gagcgctgca agatcggcgc ggcgctgcag tcgctggagt ccggcttcgc
      601 catcctgccg gccaacagcg acatgaccga ggccgaggtg aagctgcgcg acctgccggt
      661 cggcgatttc gcgctgaaga gcgcgctggc cgaggtgtcc agccagttcg actacgtgat
      721 cgtcgactgc ccgccggcgc tgtccaagct cacggtcaac gcgctggtcg ccgccgacgg
      781 cgtgctgatc ccgatgcagt gcgagtatta cgcgctggaa gggctcaccg cgctgatgga
      841 caccatcaac aagatcaagc gcgtgatcaa cccgaagctg gagatcgaag gcctgctgcg
      901 caccatgttc gatccgcgca acaacctggc caacgatgtc tcggcgcagc tgacgcagca
      961 tttcggcgac aaggtgtatc gcaccatcgt gccgcgcaac gtgcgcctgg ccgaggcgcc
     1021 cagccacggc atgccggtga tgcagtacga cgcgcagtcg tccggtgcca aggcctacct
     1081 cgcgctggcc ggcgaggtgc tgcgccgtca cgacggcgga cagctgcgct tgaactgatg
     1141 aacgtcctgg tgccgctgcc gcgccgcgat ttcgatccga ccgaggtcgc ggtcagctgg
     1201 aaaattctga gcgaagccgg acacgagctg atcttcgcca cgcctgacgg cagcgtcgct
     1261 gcggccgacc cggtgatgat ttccggcgaa ggcctcgacg tctggggttt cattccgggc
     1321 ctgaagaaac tgaagctgat cgggctggcg ctgcgcgcgc acgcgccggc gcgccgcgcg
     1381 tatgccagcc tgcagcgcga cgcgcgcttc ctcgcgccga aaaaatatgc cgaactgaac
     1441 gcagcggatt tcgacgcgct gctgctgccc ggcggccacg ccaagggcat gcgcgaatat
     1501 ctcgaagaca aaaccctgca gtcggtcgtg gccgatttct tcgatgccgc caagcccgtc
     1561 gccgcggtgt gccacggcgt ggtgctggcg gcgcgcagcc tgtcgaagaa gaccggcaag
     1621 ccggtgctgc acggcaagcg caccaccgcg ctgacctgga agctggaaaa gagcggctgg
     1681 gacctgaccc gtttcttcgc gcgtttctgg gacggcgact actaccgcac ctacggcgaa
     1741 cagccgggcc agccggccgg ctacatgagc gtgcagcagg aagtgacgcg tgcgctggcg
     1801 ctcccctctg atttccgcga cgtgccggcc gatgcgccgc accacttcat gaaagccagc
     1861 g
//
LOCUS       DASZZC010000594         5078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_303344,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000594 DASZZC010000000
VERSION     DASZZC010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303344"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VHE37_12690"
     CDS             <1..729
                     /locus_tag="VHE37_12690"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HVT36436.1"
                     /translation="QGRGWWVSGGIAAGLVLAGVVAAAAGLIAEAASGMGQEIMNAGI
                     LLAAVVMLGWHNVWMAQHGKELSAQMSALGKSVASGQQSLMALATVIALAVLREGSEV
                     VLFLYGIAAAGSGALAMFIGGAVGVALGAAAGFVLYFGLLKIPLKRFFTVTSWMILLL
                     AAGLASQAAHYLIQADLLPAMGNELWDSSRILSGESMLGRILRTLVGYDPRPAGSQLL
                     FYAIALVVIGGAMKLFGERPRAAH"
     gene            complement(880..1551)
                     /locus_tag="VHE37_12695"
     CDS             complement(880..1551)
                     /locus_tag="VHE37_12695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HVT36437.1"
                     /translation="MSEQLWDEVDDYINGLLAPADEALDGALEAADAAGLPQIAVAPN
                     QGKLLHLLAQTVGASAILEIGTLAGYSTIWLARALKPGGRVVTLESDIKHAEVANANL
                     ARAGLAGMVEIRVGRALDTLPQIAAQNRGPFDFIFIDADKASIPEYFKWALKLSRRGS
                     LIVIDNVVRKGAILDAASEDSAVRGVRRMYELIAAEPRVSATAIQTVGGKGHDGFCLV
                     RVLSG"
     gene            complement(1563..3524)
                     /locus_tag="VHE37_12700"
     CDS             complement(1563..3524)
                     /locus_tag="VHE37_12700"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT36438.1"
                     /translation="MGLHPMSHGAGSAGSMAARIQAHDWKSSPLGTPRKWPQSLRTAL
                     SIVLDAAAPMALVWGEEQLLFCNDAWQGEFGEVEPGRSFDACFPQIAEQLARPLKRVM
                     GDGQPQRADAGQRPRRHGFALSAVRGEHDSVAGVLIAPRTAPASADDALDERLTELFR
                     HAPVGIAVLRGPQHVYRYANAEYLRVVGNRDVLGRPVRSALPELAGQGAYELLDQVYA
                     SGEAYVGRSIRTRLTADPSGALKDCYFDLVFQPLRERGAVDGIVVIAFDITELAHARA
                     QAESASAAKDTFLAMLGHELRNPLAPIVTALHLMRQRDSRHMAHERTVIERQVQHMVR
                     LVDDLLDVSRITRGNVELQKTRVELADVVNPAIEMVSPLLEKLRHKLAVAVPTVGLPV
                     VVDPTRLAQVLSNLLNNAAKFTEPGGDITLVAERDGEQIVLRVTDNGIGIAEDMLPKV
                     FEMFEQGEQSLVRSRGGLGLGLTIARRLAELHGGTLEAHSEGTGKGSSFVLRLPAAPP
                     EEMPQTLYGGDREALRLLGSRVLVVDDNEDAAQILALALTAAGHTTRVAHDGPTALEL
                     AQEFKPEVALLDIGLPVMDGLELGRRLRRLPATRETRLIAVTGYGQAIDRKRSLDAGF
                     DAHLVKPVDVATLDALIKGDAAPPDVRRH"
     gene            3872..5062
                     /gene="alaC"
                     /locus_tag="VHE37_12705"
     CDS             3872..5062
                     /gene="alaC"
                     /locus_tag="VHE37_12705"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HVT36439.1"
                     /translation="MKAVFPRIQRLPPYVFNVIGDLKKAARARGEDVIDFSMGNPDQP
                     TPQHIVDKLVEVVERNDPAVYRYSVSKGIPRLRRAITKWYKSRYDVDLDPETEAIATI
                     GSKEGLAHLMLGTLDKGDVVMVPNPAYPIHPYGAVIAGADVRHIRLTPGTDFFAELEK
                     GIKECWPRPKFLILNFPGNPTAQCVELDFFERVAAMAREYDFWVIHDLAYADIAFDGY
                     KAPSFMQVKGAKDIGVEFFTLSKSYNMPGWRVGFMVGNKDLCTALARMKSYLDYGTFT
                     PIQVAAITALEGPQDCVREISNMYQDRRDVLVKGLNESGWAVETPKASMFVWARIPEA
                     FQKMGSLEFSKLLMTEAKVAVSPGIGFGEYGDDHVRFALIENEHRTRQAIRGIKKLLK
                     EGRA"
BASE COUNT          770 a         1699 c         1744 g          865 t
ORIGIN      
        1 cagggccgcg gctggtgggt gagcggcggc atcgccgcgg gcctggttct cgccggcgtg
       61 gtggcggccg ctgccgggct gatcgcggaa gccgcctccg gcatggggca ggagatcatg
      121 aacgccggca tcctgctggc cgcggtggtc atgctcggct ggcacaacgt gtggatggcc
      181 cagcatggca aggaactgtc ggcgcagatg agcgcgctgg gcaaatcggt ggccagcggc
      241 cagcagtcgc tgatggcgct ggcaacggtc atcgcgctgg cggtgctgcg cgaaggttcg
      301 gaagtggtgc tgttcctcta cggcatcgcc gccgcgggtt ccggtgcgct ggccatgttc
      361 atcggcggtg cggtcggcgt tgcgctcggc gcggcggccg gcttcgtgct gtatttcggc
      421 ctgctgaaga ttccgctgaa gcgcttcttc accgtcacca gctggatgat cctgctgctc
      481 gccgccggcc tggcctcgca ggcggcgcac tacctgatcc aggcggacct gctgcccgcg
      541 atgggcaacg agctgtggga cagctcgcgc atcctctcgg gcgaatcgat gttggggcgc
      601 atcctgcgca cgctggtggg ctatgacccg cgcccggccg gcagccagct gctgttctac
      661 gccatcgcgc tggtggtgat cggtggggcg atgaagctgt tcggcgagcg gccccgcgcc
      721 gcgcactagg gctcacatcc cgaagggttg cgcgccaaac agcgcaatgc ggcgttgctc
      781 gtcgcttgtt tggaccgacc aaaccgcgct cgtcgcgcct tgcctggcgc tgtttggcgc
      841 agcaacgctc gcgcgaaatt ggcgttaaca ggccctagtt cagccggaca gcacgcgcac
      901 caggcagaag ccgtcgtggc ccttgccgcc gacggtctgg atggcggtgg ccgacacgcg
      961 cggctcggcg gcgatcagtt cgtacatgcg gcgtacgccg cgcaccgcgc tgtcctcgct
     1021 cgccgcatcg aggatcgcgc ccttgcgcac cacgttgtcg atgacgatca ggctgccgcg
     1081 gcgcgacagc ttcagcgccc acttgaagta ttccgggatg ctggccttgt cagcgtcgat
     1141 gaagatgaaa tcgaacggcc cgcggttctg tgccgcgatc tgcggcagcg tgtcgagcgc
     1201 acgtccgacg cggatctcca ccatgccggc cagcccggcg cgcgccaggt tggcattggc
     1261 cacctccgca tgcttgatgt cggactccag cgtgaccacg cgcccgcccg gcttgagcgc
     1321 gcgcgccagc cagatggtgc tgtagccggc cagcgtgccg atctcgagga tggcgctggc
     1381 gcccaccgtc tgcgccagca ggtgcagcag cttgccctgg ttgggggcca ccgcgatctg
     1441 cggcaggccg gcggcatcgg ccgcttccag tgcgccgtcg agcgcctcgt cggccggcgc
     1501 cagcaggccg ttgatgtagt catccacttc gtcccacagc tgttcgctca tgtcgcgcgt
     1561 tcctagtgcc gccgtacatc gggcggtgct gcatctcctt tgatgagggc gtcgagcgtg
     1621 gcgacgtcta ctggttttac gagatgtgcg tcgaagccgg catcgagcga acgcttgcgg
     1681 tcgatggcct ggccgtagcc ggtgaccgcg atcagccgcg tttcgcgcgt ggccggcagg
     1741 cggcgcaggc ggcggccgag ttccagtccg tccatcaccg gcaggccgat gtcgagcagc
     1801 gccacctcgg gcttgaactc ctgcgccagt tccagcgcag tggggccgtc gtgcgccacg
     1861 cgcgtggtgt ggccggcggc ggtcagcgcc agcgccagga tctgcgcggc gtcctcgttg
     1921 tcatccacca ccagcacgcg cgagccgagc aggcgcagcg cctcgcggtc gccgccgtac
     1981 agcgtctgcg gcatttcttc aggcggcgcg gccggcaggc gcagtacgaa gctgctgccc
     2041 ttgccggtgc cttcgctgtg cgcttccagc gtgccgccgt gcagctccgc caggcgccgc
     2101 gcgatggtca gccccaggcc caggccgccg cgcgagcgca ccagcgactg ctcgccctgc
     2161 tcgaacatct cgaacacctt gggcagcatg tcctcggcga tgccgatgcc gttgtcggtc
     2221 acgcgcagca cgatctgctc gccgtcgcgc tcggcgacca gcgtgatgtc gccgccgggc
     2281 tcggtgaact tggccgcgtt gttgagcagg ttggacagca cctgcgccag ccgcgtcggg
     2341 tcgaccacca ccggcaggcc cacggtgggc accgccaccg ccagcttgtg gcgcagcttt
     2401 tccagcagcg ggctgaccat ctcgatggcg ggattgacca catcggccag ctcgacgcgc
     2461 gtcttctgca attccacgtt gccgcgggtg atgcgcgaga cgtcgagcag gtcgtcgacc
     2521 aggcgcacca tgtgctgcac ctggcgctcg atcaccgtgc gctcgtgcgc catgtggcgg
     2581 ctgtcgcgct ggcgcatcag gtgcagcgcg gtgacgatgg gcgccagcgg gttgcgcagc
     2641 tcgtggccga gcatggcgag gaaggtgtcc ttggccgcgc tggccgattc cgcctgcgcg
     2701 cgggcgtgcg ccagctcggt gatgtcgaag gcgatgacca cgatgccgtc gaccgcgccg
     2761 cgctcgcgca gcggctggaa caccaggtcg aagtagcagt ccttcagcgc gccgctggga
     2821 tcggcggtga ggcgcgtgcg gatcgagcgg cccacgtagg cctcgccgct ggcgtacacc
     2881 tggtcgagca gctcgtaggc gccctggccg gccagttcgg gcagcgcgct gcgcaccggc
     2941 cggccgagca cgtcgcggtt gccgaccacg cgcaggtact cggcgttggc gtagcggtac
     3001 acatgctgcg gtccgcgcag cacggcgatg cccactggcg cgtggcggaa cagttcggtc
     3061 agccgctcgt cgagcgcgtc gtcggcggat gcgggcgccg tgcgcggcgc gatcagcacg
     3121 ccggcgacgc tgtcgtgctc gccgcgcacg gcggacagcg caaagccatg gcgccgcggg
     3181 cgctggccgg catcggcgcg ctgcggctgg ccgtcgccca tcacccgctt gagcggccgc
     3241 gccagctgtt cggcgatctg cggaaagcag gcgtcgaagc tgcgcccggg ctcgacctcg
     3301 ccgaactcgc cctgccaggc gtcgttgcag aacagcagct gttcctcgcc ccacaccagc
     3361 gccatcggcg cggcggcgtc aagcacgatg gacagcgcgg tgcgcaggct ctgcggccat
     3421 ttgcgtggcg tgcccagcgg cgatgatttc cagtcgtgcg cctggatgcg cgcggccatg
     3481 ctgccggcgg aaccggcgcc gtggctcatc gggtgcagcc ccatgcagtg tcttccatga
     3541 tcgttcgggc ggcgggcgaa gtgggcgcga tgatggcatg cggcgctacg cgcctcaatg
     3601 cgaaatctcg cacgccggca tgagacttcg ccgcaccgtg caaggttgca gcggcgtgaa
     3661 aatgatgcca ccgcacccgg gttttcgatg ttacaaaata atgtacatat tattagttaa
     3721 taaatatttc ataacacgat gaagggcggt gcggtggatg gtttttccgc attttccgcg
     3781 cgcacttccg gcatcgcggc ggctctggtc atcctcgcgc gctgcctcga taatacgcgg
     3841 ccttttttcc cacccatcgc aggaatccca agtgaaagct gtcttcccgc gcatccagcg
     3901 cctgccgccc tacgtcttca atgtcatcgg tgacctgaag aaggcggctc gagctcgtgg
     3961 cgaagacgtc atcgatttct cgatgggcaa cccggaccag ccgacgccgc agcacatcgt
     4021 cgacaagctg gtggaagtgg tggagcgcaa cgacccggcg gtgtaccgct actcggtgag
     4081 caagggcatt ccgcgcctgc gtcgcgccat caccaagtgg tacaagagcc gctacgacgt
     4141 cgatctcgat cccgagaccg aggccatcgc caccatcggt tccaaggaag ggctggcgca
     4201 cctgatgctc ggtacgctcg acaagggcga cgtggtcatg gtgcccaacc cggcgtaccc
     4261 gatccacccg tacggagcgg tcatcgccgg cgccgacgtg cgccacatcc gcctgacgcc
     4321 gggcacggat ttcttcgctg agctggagaa gggcatcaag gaatgctggc cgcggcccaa
     4381 gttcctcatc ctgaattttc ccggcaaccc cacggcacag tgtgtcgagc tggatttctt
     4441 cgagcgcgtg gcggcgatgg cacgcgagta cgacttctgg gtgatccacg acctggccta
     4501 cgctgacatc gccttcgacg gctacaaggc gccgagcttc atgcaggtga aaggcgccaa
     4561 ggacatcggc gtggaattct tcaccctgtc caagagctac aacatgcccg gctggcgcgt
     4621 cggcttcatg gtcggcaaca aggacctgtg caccgcgctg gcgcgcatga agagctatct
     4681 cgactacggc accttcacgc cgatccaggt ggcggcgatc accgcgctgg agggcccgca
     4741 ggactgcgtg cgtgaaatct ccaacatgta ccaggaccgc cgcgacgtgc tggtcaaggg
     4801 tctcaacgaa tccggctggg cggtggaaac gcccaaggcc tcgatgttcg tgtgggccag
     4861 gattcccgag gcgttccaga agatgggcag cctggagttt tccaagctgc tgatgaccga
     4921 ggccaaggtg gcggtgtcgc ccggaatagg ctttggcgaa tacggcgacg accacgtgcg
     4981 cttcgcgctg atcgagaacg agcaccgcac gcgccaggcc atccgcggca tcaagaagct
     5041 gctcaaagag ggccgcgcat gaccacctcg ccggtgaa
//
LOCUS       DASZZC010000595         5114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_743640,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000595 DASZZC010000000
VERSION     DASZZC010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743640"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..543
                     /locus_tag="VHE37_12710"
     CDS             241..543
                     /locus_tag="VHE37_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36440.1"
                     /translation="MTASRSGANAPVRRWAQVVDRAVLGITLIAVHEADALAAHWTEL
                     GERLEVARRARGPRQLIREQLDLLPESHSRLRHDQQIRRQLWRGLWRDLSAVTAAH"
     gene            complement(537..830)
                     /locus_tag="VHE37_12715"
     CDS             complement(537..830)
                     /locus_tag="VHE37_12715"
                     /inference="COORDINATES: protein motif:HMM:NF040443.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HVT36441.1"
                     /translation="MQIRVEVEVTPEELRRFLGLPDVTGLQDDIVAFLRDKVGAASEF
                     DASAFLKANLDALKRNELLNKIVARVRVTDPAKADEKPAPKAARPRKRKTAAQ"
     gene            complement(841..1740)
                     /locus_tag="VHE37_12720"
     CDS             complement(841..1740)
                     /locus_tag="VHE37_12720"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HVT36442.1"
                     /translation="MHTEPRTEPLFESSIKSLPLIHRGKVRDIFALGDAHMLIVTSDR
                     LSAFDVVLPRPIPGKGAVLNALTDFWMRRFSTLLPNHSAQMDLSKYLTPAELAECAGR
                     AVVVKKLKALPVECVARGYLIGSGWKDYQKTGAVCGIPLPAGLRIADQLPEPIFTPAD
                     KAPAGKHDENISYAQVEEKVGRDLARQLRDVTLRIYSEASAYARERGIIVADTKFEFG
                     VDADNRLHLIDEVLTPDSSRFWPADTYAPGTSPPSFDKQFVRDYLETLDWNKQAPGPW
                     LPDDVVQKTAAKYREAQRLLAGA"
     gene            complement(1730..2173)
                     /locus_tag="VHE37_12725"
     CDS             complement(1730..2173)
                     /locus_tag="VHE37_12725"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HVT36443.1"
                     /translation="MSLVIALHVLASTLWVGGMFFAYACLRPATASTDVPVRVQVWER
                     ALRRFFRWIWISIALLLLTGFWMFFGEKIRGPHVHLMMGLGMVMMLLAAHVYFAPYKR
                     LRRCVGVHNWQEGAKQLNQIRTFVAINLALGLIVVAVAAGGRYAH"
     gene            2272..2952
                     /gene="rpe"
                     /locus_tag="VHE37_12730"
     CDS             2272..2952
                     /gene="rpe"
                     /locus_tag="VHE37_12730"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HVT36444.1"
                     /translation="MSKSKLAPLIAPSILSADLARLGDDVARVLAAGADIVHFDVMDN
                     HYVPNLTFGPAVCAALKKYGITAPLDVHLMVEPVDALAAEFARAGASYITFHPEASRH
                     IDRSLAAIRDAGCKSGLVFNPATPLDCLEYVLDKVDMILIMSVNPGFGGQSFIPSALD
                     KLRKAREMIDASGLPIRLEIDGGVKTDNIGAIASAGADTFVAGSAIFNSKDYAATIRA
                     MRDAIARG"
     gene            3112..4548
                     /gene="trpE"
                     /locus_tag="VHE37_12735"
     CDS             3112..4548
                     /gene="trpE"
                     /locus_tag="VHE37_12735"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HVT36445.1"
                     /translation="MEPQRYNRIALTRELPGDLDTPVGAYLKLGNRPYSFLLESMQGG
                     ERWGRYSFIGLPCREIVTVRGHQLTVTRDDQIVEQRELADPIAWLRDYAARTKVKPEP
                     TLPRFTGGLGGYFGYDCVRYFEPKLDHPQPDTLNTPDILLMYADELAIFDALRATLTL
                     VVHANADDPADQARARQRLNQIEAQLALPLPQSPTAGAPARLNFSTSFERGPYLTAVA
                     KAKEYIAAGDTFQVQLGQRLSAPFAAQGVELYRAIRRLNPSPYLYCFHMGDHHVVGSS
                     PEILVRVQNGEVTLRPIAGTRPRGATPEDDLRLEKELLADPKERAEHVMLIDLGRNDV
                     GRVCEAGVKVTDQMVIERYSHVMHIVSNVTGKLKAGLDPLDVLAATFPAGTLTGAPKV
                     RTMEIIQELEPVKRGIYGGAVGYLGWDGNMDLAIAIRTAVIKDGKVHVQASAGIVADS
                     IPQAEWDETLHKARAMMRAAEIAAGAAS"
     gene            4545..>5114
                     /locus_tag="VHE37_12740"
     CDS             4545..>5114
                     /locus_tag="VHE37_12740"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HVT36446.1"
                     /translation="MILMIDNYDSFTYNLVQYLQELGAEVQVQRNDALTVAQVAALKP
                     THIVLSPGPCTPNEAGICLELIARLHTQFPILGVCLGHQAMGQAFGGVVKRARQLMHG
                     KVSAIHHTGKGVFRGLPSPYTATRYHSLIVEQETLPSCLEVTAWTQHDGGARDEIMGL
                     KHKTAPIEGVQFHPESILTEHGHALLKNFL"
BASE COUNT          909 a         1706 c         1662 g          837 t
ORIGIN      
        1 ccaagactgg cgacgaactg gccaagacct tcaagcaagg ttacgagacc gtgtccgcca
       61 agttcgccgg cgagccggtg gtgaagaagg tcaagagcac cgtgcgcaag gccggcacca
      121 aggcgcgcaa ggctgccaag gccgctgcct aagagttttg atgatctcct ccgccattcc
      181 tcaatgaatg gcattggggc cgggggcaac ctcggccctt ttttatttgg agagatgaag
      241 atgactgcat ccaggtccgg cgcgaatgcg ccggtgcggc gttgggcgca agttgtggat
      301 cgcgcggtac tcggcatcac gctgatcgcc gtgcacgaag ccgatgcgct tgccgcccat
      361 tggaccgaac tgggcgaaag actcgaagtg gcgcgccgtg cgcgcgggcc gcgccagctg
      421 atccgcgagc agctcgacct gctgccggaa tcgcacagcc ggctccggca cgaccagcag
      481 atccgtcgcc agctctggcg cggattgtgg cgcgacttgt cagcggtgac cgccgctcac
      541 tgagccgcgg ttttgcgctt gcgtgggcgt gccgctttcg gagcgggctt ctcgtctgct
      601 ttcgcgggat cggtcacgcg cacgcgcgcg acgatcttgt tgagcagctc gttgcgcttg
      661 agcgcatcga gattggcctt gaggaaggcg ctggcgtcga attcgctggc ggcgccgacc
      721 ttgtcgcgca ggaaggcgac gatgtcgtcc tgcagcccgg tcacgtcggg caggccgagg
      781 aaacgacgca gctcttccgg cgtgacttcc acttccaccc tgatctgcat gggttactcc
      841 ttatgcgccg gcgagcaggc gctgcgcttc gcggtacttg gcggcggttt tctgcaccac
      901 gtcgtcgggc agccacggtc ctggcgcctg cttgttccag tccagtgttt ccaggtaatc
      961 gcgcacgaac tgcttgtcga agctcggtgg gctggtgccc ggcgcgtagg tgtcggccgg
     1021 ccagaagcgc gaggaatccg gtgtcagcac ctcgtcgatc aggtgcagcc ggttgtcggc
     1081 gtcgacgccg aactcgaact tggtatcggc cacgatgatg ccgcgctcgc gcgcataggc
     1141 cgaggcttcg ctgtagatgc gcagcgtcac gtcgcgcagc tggcgggcga gatcgcgtcc
     1201 gactttctcc tcgacttgcg cataggagat gttctcgtcg tgcttgcctg ccggcgcctt
     1261 gtcggccggc gtgaagatcg gttcgggcag ctggtcagcg atgcgcaggc cagccggcag
     1321 cgggatgccg cacaccgcgc cggtcttctg gtaatccttc cagccggagc cgatcaggta
     1381 accgcgcgcg acgcattcca ccggcagcgc tttcagcttc ttcaccacca ccgcgcggcc
     1441 ggcgcactcg gccagttctg ccggcgtcag gtacttcgac aggtccatct gcgccgaatg
     1501 attcggcagc agcgtggaga agcgccgcat ccagaagtcg gtcagcgcgt tgagcaccgc
     1561 gcccttgccg ggaatcggcc gcggcagcac cacgtcgaac gcggacaggc gatcgctggt
     1621 gacgatcagc atgtgcgcgt cgcccagcgc gaagatgtcg cgcaccttgc cgcgatggat
     1681 cagcggcagg ctcttgatgg acgattcaaa caggggttcg gtacggggtt cagtgtgcat
     1741 agcgtccacc ggcggcgacc gcgaccacga tcagcccgag tgcgaggttg atggcgacga
     1801 aggtgcggat ctggttgagc tgcttcgccc cttcctgcca gttgtgcacg ccgacgcagc
     1861 ggcgcaggcg cttgtatggc gcgaagtaca cgtgggcggc gagcagcatc atgaccatgc
     1921 ccaggcccat catcaggtgc acgtgcgggc cgcggatctt ctcgccgaag aacatccaga
     1981 aaccggtgag cagcagcagc gcaatggaaa tccagatcca gcggaagaag cggcgcagcg
     2041 cgcgctccca tacctgcacg cgcaccggca catcggtgga agcggtggcc ggccgcaggc
     2101 aggcgtaggc gaagaacatg ccgccgaccc agagagtgga ggcaagcacg tgcagggcaa
     2161 tgaccaggct catggcggcg ggcgtaaaag tgaagcggca ctctaccaaa gcgctcacac
     2221 ggcagcgcgc tttgcaggga taatacgcac cgtttccggg tttccgactg catgtccaag
     2281 agcaagctgg caccgctgat tgcgccctcg atcctgtccg ccgatctggc gcggctgggc
     2341 gatgacgtgg cgcgcgtgct cgccgccggc gccgacatcg tgcacttcga cgtgatggac
     2401 aaccattacg tgcccaatct caccttcggc cccgcggtgt gcgcggcgct gaagaaatac
     2461 ggcatcaccg cgccgctcga cgtgcacctg atggtggagc cagtcgatgc gctcgccgcc
     2521 gagttcgcca gggccggcgc cagctacatc accttccacc cggaagcgtc gcgccacatc
     2581 gaccgctcgc tcgccgccat ccgcgacgcc ggctgcaagt ccggcctggt gttcaacccg
     2641 gccacgccgc tggactgcct cgagtacgtg ctcgacaagg tggacatgat cctgatcatg
     2701 tccgtgaacc ccggcttcgg cgggcagagc ttcatcccca gcgcgctcga caagctgcgc
     2761 aaggcgcgcg agatgatcga tgccagcggg ctgccgatcc gcctggagat cgacggcggc
     2821 gtgaagaccg acaacatcgg cgccatcgcc agcgccggcg ccgacacctt cgtcgccggc
     2881 tcggccatct tcaattccaa ggattacgcc gcgaccatcc gcgccatgcg cgacgccatt
     2941 gcgcgcggct gatcatgcgt ttccacgagc aaaaccgcct gcaggaccac caaccgcact
     3001 ggcgatggtg acgttataaa ttatcatctg tttttacaat aagacattaa tttataacag
     3061 ctcgaactgc catgcggtga gagctaaaaa tcatcgaagg acatctgcac gatggaaccg
     3121 caacgctaca accgcatcgc gctcacgcgc gagctgcccg gcgatctcga caccccggtg
     3181 ggcgcgtatc tcaagctcgg caaccggccg tattcctttc tgctcgaatc gatgcagggc
     3241 ggcgagcgct ggggccgcta ctcgttcatc ggcctgccgt gccgcgagat cgtcaccgtg
     3301 cgcgggcacc agctgacggt gacgcgcgac gaccagatcg tcgaacagcg cgagctggcc
     3361 gaccccatcg cctggctgcg cgactacgcg gcgcgcacga aggtgaagcc cgagcccacg
     3421 ctgccgcgct tcaccggcgg gctgggcggc tacttcggct acgactgcgt gcgctacttc
     3481 gagcccaagc tcgatcaccc gcagccggac acgctgaaca cgcccgacat cctgctgatg
     3541 tacgccgacg aactggccat cttcgatgcg ctgcgcgcca cgctcacgct ggtggtgcat
     3601 gccaacgccg acgatccggc ggaccaggcg cgcgcgcgcc agcggctcaa ccagatcgag
     3661 gcgcaactcg cgctgccgct gccgcagtcg cccaccgcgg gcgcaccggc ccggctgaat
     3721 ttttccacca gcttcgagcg cggcccgtat ctcaccgcgg tggccaaggc caaggagtac
     3781 atcgccgccg gcgatacctt ccaggtgcag ctcggccagc gtttgtccgc gccttttgcg
     3841 gcgcagggcg tggagctgta ccgcgccatc cgccggctca atccgtcgcc gtatctctat
     3901 tgcttccaca tgggcgacca ccacgtggtc ggctcctcgc cggaaatcct ggtgcgcgtg
     3961 cagaacggcg aagtcacgct gcgccccatt gccggaacca ggccgcgcgg cgccacgccc
     4021 gaggatgacc tgcgcctgga gaaggaactg ctggccgatc ccaaggagcg cgccgagcac
     4081 gtgatgctga tcgacctggg ccgcaacgac gtgggccgcg tgtgcgaggc cggcgtgaag
     4141 gtcaccgacc agatggtgat cgagcgctac agccacgtca tgcacatcgt gtccaacgtc
     4201 accgggaaac tgaaagccgg gctcgacccg ctcgacgtgc tcgccgcgac attccccgcc
     4261 ggcacgctca ccggcgcgcc caaggtgcgc accatggaaa tcatccagga gctggaaccg
     4321 gtcaagcgcg gcatctacgg cggcgcggtc ggctatctcg gctgggacgg caacatggac
     4381 ctggccatcg ccatccgcac cgcggtgatc aaggacggca aggtgcacgt gcaggcctcg
     4441 gccggcatcg ttgcggattc gattccgcag gccgagtggg atgaaacgct gcacaaggcg
     4501 cgggccatga tgcgcgccgc ggaaatcgcg gcgggagcgg cgtcatgatc ctgatgatcg
     4561 ataattacga ttccttcacc tacaacctgg tgcagtacct gcaggaactc ggcgccgagg
     4621 tgcaggtgca gcgcaacgac gccctcaccg tggcgcaggt cgccgcgctc aagcccacgc
     4681 acatcgtgct gtcgccgggg ccgtgcacgc cgaacgaagc cggcatctgc ctggaactga
     4741 tcgccaggct gcacacacag tttccgatac tcggtgtctg cctcggtcac caggcgatgg
     4801 gccaggcgtt cggcggcgtg gtcaagcgcg cacgccagct gatgcacggc aaggtgtccg
     4861 ccatccacca caccggcaag ggcgtgttcc gcggcttgcc gtcgccgtac acggccacgc
     4921 gctaccactc gctgatcgtg gagcaggaga cgctgccatc gtgcctggaa gtcaccgcgt
     4981 ggacgcagca cgacggcggc gcgcgcgacg agatcatggg gctgaagcac aagacggcgc
     5041 cgatcgaggg ggtgcagttc cacccggaat cgatcctcac cgagcacggg catgcgctgc
     5101 tgaagaactt cctg
//
LOCUS       DASZZC010000596         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_870993,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000596 DASZZC010000000
VERSION     DASZZC010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870993"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VHE37_12745"
     CDS             <1..563
                     /locus_tag="VHE37_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT36447.1"
                     /translation="AASGEEALETTRTKIPDVVLMDINMPGIGGLEATRRLTQRLPQV
                     KVIVVSMHLEEPYPSRMLAAGAAGYISKDSAADEAIAAIRRVYAGGHYVAADVAGNMA
                     ASLVKGKSDSPFDQLSQRETQVMLMVTKGHSTQDISDRLHLSPKTVSTYRYRLFEKLN
                     VSNDVELTRMAMRYGLLDDKTDVPQT"
     gene            704..1639
                     /locus_tag="VHE37_12750"
     CDS             704..1639
                     /locus_tag="VHE37_12750"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36448.1"
                     /translation="MKPPSSWRWRLLIRVSNGLRKLGLFPAYDTMVKWPVARRKAIAP
                     APWMTYPPPASVRMSRETIESRGGPLQLKWFRTAPSQAPSILFIHGGGWVMGGADALD
                     YLCANLCERLGVGVVAVDYRLAPEDRFPAGLEDCYDALNWVAARAPGPVAVIGDSAGG
                     NLTAAVCLLARERGGPKVAHQTIIYPILDATLASESMTTHQQYGATRDDMATVVRLYV
                     GDHDRRDPLVSPILAPDHRGLPPAFIITADCDLLRDDGARYAQCLRKAGVPVRYKNYL
                     EATHAFLSHPKLGYAGAEALDDIIEEMRPYLSRQA"
     gene            1636..>1975
                     /locus_tag="VHE37_12755"
     CDS             1636..>1975
                     /locus_tag="VHE37_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36449.1"
                     /translation="MSDPLELEWRWSDPRVLGETARQEIEARVALAQQRLREVHYPEV
                     PSAGECHLSDAVPAEVPTNPRASAGIASPLLEEARQLSLYPDYKYLSDLRRRARNEAE
                     YARWQERFERM"
BASE COUNT          333 a          683 c          671 g          288 t
ORIGIN      
        1 aggctgcgag cggcgaggaa gcactcgaga ccacgcgcac caagattccc gacgtggtgc
       61 tgatggacat caacatgccc ggcatcggcg gcctggaggc cacgcggcgg ctgacgcagc
      121 gcctgccgca ggtgaaggtg atcgtggtgt ccatgcacct ggaagagccg tatccgtcgc
      181 gcatgctcgc tgccggcgcc gccggttaca tcagcaagga ttccgcggcc gacgaagcca
      241 tcgccgccat ccgccgcgtg tatgcgggcg ggcactacgt ggccgccgac gttgccggca
      301 acatggccgc gagcctggtc aagggcaaga gcgattcgcc gttcgaccag ttgagccagc
      361 gcgagacgca ggtgatgctg atggtgacca agggccactc aacccaggac atctccgacc
      421 gcctgcacct gtcgcccaag acggtgagca cctaccgcta ccgcctgttc gagaagctca
      481 acgtgtccaa tgacgttgag ctcacgcgca tggccatgcg ctacggcctg ctcgacgaca
      541 agaccgacgt gccgcagacc tgagcggcgc aggcgctccg gcgccgccga ggcacgccca
      601 cgacttcctg cctgaaccgg cctgcgcgtg cgggcacggc ccgccatttg cgcttttatg
      661 aaaccgattt cataatttcg ccgcagtggg ggagaaaccg acaatgaaac cgccgtcgag
      721 ctggcgctgg cgcctgctga tccgcgtgag caatgggctg aggaagctgg ggctgttccc
      781 cgcctacgac accatggtca agtggccggt ggccaggcgc aaagccatcg cgccggcgcc
      841 gtggatgacg tatccgccgc ccgcgtcggt gcggatgtcg cgcgagacaa tcgagagccg
      901 cggcgggccg ctgcagctga agtggtttcg cacagcaccg tcgcaggcgc cttcgatcct
      961 gttcatccat ggcggcggct gggtcatggg cggcgccgac gcgctggatt acctctgcgc
     1021 caacctgtgc gagcgcttgg gcgtgggcgt ggtcgccgtc gattaccgcc tcgcgccgga
     1081 agacaggttc cccgccgggc tggaggactg ctacgacgcg ctgaactggg tggccgcgcg
     1141 cgcgcccggc ccggtcgcgg tgatcggcga cagcgccggc ggcaacctca cggccgcggt
     1201 gtgcctgctg gcgcgcgagc gcggcgggcc gaaggtggcg caccaaacca tcatctatcc
     1261 catcctcgac gccacgctgg cgtcggagtc gatgactacg caccagcaat acggcgccac
     1321 ccgcgacgac atggcgacgg tggtacgtct gtacgtcggc gatcacgatc ggcgcgatcc
     1381 gctggtgtcg ccgatcctgg cgccggatca ccgcggcctg ccgccggcct tcatcatcac
     1441 cgccgactgc gacctgctgc gcgacgacgg cgcgcgctac gcgcagtgcc tgcgcaaggc
     1501 cggcgtgccg gttcgctaca agaactacct cgaagctacg cacgcgttcc tgtcgcaccc
     1561 gaaactgggc tacgccggcg ccgaggcgct ggacgacatc atcgaagaga tgcgcccgta
     1621 cctgagccgg caagcgtgag cgatccgctg gaactggaat ggcgctggtc cgatccgcgc
     1681 gtgctgggcg agaccgcacg ccaggagatc gaagcgcgcg tggcgctggc gcagcagcgc
     1741 ctgcgcgagg tgcactaccc ggaagtgccg tccgcgggcg agtgccatct ctcggacgcc
     1801 gtgccggccg aggtcccgac gaaccccagg gcatccgccg gcatcgccag ccccttgctg
     1861 gaagaagcgc ggcagctgtc gctgtacccg gattacaaat atctctccga cctgcgccgc
     1921 cgcgcacgca acgaggccga gtacgcgcgc tggcaggagc gcttcgagcg catgc
//
LOCUS       DASZZC010000597         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1143471,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000597 DASZZC010000000
VERSION     DASZZC010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143471"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHE37_12760"
     CDS             <1..355
                     /locus_tag="VHE37_12760"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HVT36450.1"
                     /translation="AGVQPALFIDADLAQLAAALDSGAPHIEIHTGAYADAKGAARAA
                     ERRRIAAFARAAHEAGLEVHAGHGLTLDNVGPIAKVPQIVELNIGHSIVADALLHGMA
                     GAVRRMRAAMRAART"
     gene            363..743
                     /gene="acpS"
                     /locus_tag="VHE37_12765"
     CDS             363..743
                     /gene="acpS"
                     /locus_tag="VHE37_12765"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HVT36451.1"
                     /translation="MIHGIGVDIVAHARIEKMYARHGARLARRILSARELEEFRVTRK
                     QPAHYLASAFAVKEAFVKALGTGFRGVTHRDPGVVRDELGKPSLIYSRALARRLKALG
                     IGGGHVSLSDDGGMVCAYVILEKA"
     gene            complement(847..>1572)
                     /locus_tag="VHE37_12770"
     CDS             complement(847..>1572)
                     /locus_tag="VHE37_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT36452.1"
                     /translation="IRLSGADPPREEPLAGKFVLIADNNLPNRRYIGMLCEELGLDIS
                     EAADGSEALMQWQDLRPEFVILDARMPLLDGAACAQQIRAVEKPGEHCRIVAASAHLE
                     PEERAAFLAAGADAILIKPFTSEQLLHALMPTRQEQPPAVSEMLTSDPEMLQLLAEEL
                     PKQYAELEEALRQADTRAARDAAHQLHGTAAFYHLAELKRACANLERKLAGAGPAALP
                     LPGADAVKQILQQTLATLAARKP"
BASE COUNT          245 a          518 c          554 g          255 t
ORIGIN      
        1 cgcgggcgtg cagccggcgc tgttcatcga tgccgatctg gcgcagctgg ccgcagcgct
       61 cgacagcggg gcgccgcaca tcgagatcca caccggcgcc tatgccgatg cgaaaggtgc
      121 ggcacgcgca gcggaacgca gacgcatcgc cgccttcgcg cgcgccgcgc acgaagcggg
      181 gctggaagtg catgccgggc atggactcac gctcgacaac gtcggaccga tcgccaaggt
      241 tccgcagatc gtcgagctga acatcggcca cagcatcgtc gctgatgcgc tgctgcacgg
      301 catggccggt gcggtgcggc gcatgcgcgc ggccatgcgc gccgcgcgga cgtgagcgcg
      361 gcatgatcca cggcatcggc gtcgatatcg tcgcccacgc gcgcatcgag aagatgtacg
      421 cgcgccacgg cgcgcggctg gcgcggcgca tcctgagcgc gcgcgagctc gaggaattcc
      481 gcgtcacgcg caagcagccg gcgcattacc tggccagcgc cttcgcggtg aaggaggcct
      541 tcgtcaaggc gctcggcacc gggttccgcg gagtcaccca ccgcgatccc ggcgtcgtgc
      601 gcgacgaact gggcaagccc tcgctgatct attcgcgcgc gctggcgcgc aggctcaagg
      661 cgctgggcat cggtggcgga cacgtgtcgc tgtccgacga cggcggcatg gtctgcgcgt
      721 acgtgatcct ggagaaagcc tgaaggccgt tgttttgcct caccgcaccc ggggttggcg
      781 tgttataaaa aatatactta aagtaaaaat ttacaatatt taataacatc aaaatgggca
      841 gtgcggtcag ggcttgcgcg ccgccagcgt ggccagggtc tgctgcagga tctgcttcac
      901 cgcgtctgca ccgggcaagg gcagcgccgc cgggccggcg ccggcgagct tgcgttcgag
      961 attggcgcag gcccgcttga gttcggccag gtggtagaac gcggcggtgc catgcagctg
     1021 gtgcgccgca tcgcgcgcgg ccctggtgtc ggcctggcgc aaggcttctt ccagttcggc
     1081 gtattgcttg ggcagctctt cggccagcag ctgcagcatc tcgggatcgc tggtcagcat
     1141 ttccgacacc gccggcggct gctcctgccg cgtcggcatc agcgcgtgca gcagctgctc
     1201 gctggtgaag ggcttgatca ggatggcatc ggcgccggcc gcgagaaacg ctgcgcgctc
     1261 ctccggctcc aggtgcgcgc tggcggcgac gatgcggcaa tgctcgccgg gcttctccac
     1321 cgcgcggatc tgctgcgcgc aggcggcgcc gtcgagcagc ggcatgcgcg cgtcgaggat
     1381 gacgaactcc ggccgcaggt cctgccactg catcagcgcc tcggagccat ccgcggcttc
     1441 gctgatatcc agcccgagtt cctcgcacag catgccgatg taacggcggt tgggcaggtt
     1501 gttgtcggcg atcagcacga acttgccggc cagcggttcc tcgcgcggcg ggtcggcgcc
     1561 ggacagacgg at
//
LOCUS       DASZZC010000598         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_372089,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000598 DASZZC010000000
VERSION     DASZZC010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372089"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2429
                     /locus_tag="VHE37_12775"
     CDS             <1..2429
                     /locus_tag="VHE37_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36453.1"
                     /translation="EAEAQEQLVIAEDNAQAEQRKWDSFVQDAEAPLAQTEAERVRVQ
                     QLERARFQSEERYKRLESEHGSLNLQPIQSSLFDADTDLTNLAKEIEQAQARLAELDA
                     ELQRLRDERGQAEGALNETRQSLAAARGRMSSLETLQQAALRQDDAELSTWLRQHQLA
                     ELPRLASALRVEAGWETAVEHVLADLLQAPLLPEFAQQAATVSVAPKSGAALLSAAAG
                     SGGAGLAAKVQGPQAVLDLLSSIHLAGTADEARTRAASLAPGESVISADGVWRGRSWV
                     RYPRSGDGESGVIARGQLLRELKVQVEQQTQTLATREQALRELSQRMQAAETERREVG
                     GKFDQSRGRHAQRLAFRQAQAVRLEQAQARVQALVQELDALRQSREQQASELSESKQR
                     LVTLEATANELRNQRAGMQQALATKRDLVSRARQALLLSQQARGQVQIDLASHKSSLS
                     ALEQSIKDRGAQRETLVARRSDEERAAGELDTPLAAQAHEVEIARAAVNQAREALRAA
                     REKLEAVEASFAGILQAARAADVAKDAAQERVQAVKLEFENLRARREGYNAQIAETGF
                     EREPLFAGLKDDATPEAWEEKLASILRRIERLGPINLAAIQELEEAQAREKYIGEQYA
                     DLTNALSTLEEAIRKIDAETKELFRQTFDKVDTIFREAFPRLFGGGEAYLELTGDDLL
                     DTGVRVMARPPGKRNSSIQLLSGGEKAMTAVALLLALFQLTPAPFCLMDEVDAPLDDA
                     NVVRFTDVVREMSKNVQFIIITHNKITMELAQQLHGVTMQEPGVSRLVSVDVAQAVEL
                     AGKNEVKEVS"
BASE COUNT          437 a          807 c          901 g          314 t
ORIGIN      
        1 aggaagccga ggcgcaggag cagctggtca tcgccgagga caacgcgcag gccgagcagc
       61 gcaagtggga cagcttcgtg caggatgccg aggcgccgct ggcgcagacc gaggccgagc
      121 gcgtgcgcgt gcagcagctg gagcgggcgc gcttccagtc ggaagaacgc tacaagcgcc
      181 tggagagtga gcacggttcg ctcaacctgc agccgatcca gtccagcctg ttcgatgccg
      241 ataccgacct gaccaacctg gcgaaggaga tcgagcaggc gcaggcgcgg ctggccgaac
      301 tcgacgccga actgcagcgc ctgcgcgacg agcgcggcca ggccgagggc gcgctcaacg
      361 aaacgcgcca gtcgctggcc gccgcgcgcg gccgcatgtc gtcgctggag acgctgcagc
      421 aggcggcgct gcgccaggac gatgccgaac tgtccacctg gctgcgccag caccagctgg
      481 ccgaactgcc gcgactggcc agcgcgttgc gcgtcgaggc gggctgggaa accgcggtcg
      541 agcacgtgct ggccgatctg ctgcaggcac cattgctgcc ggaattcgcg cagcaggctg
      601 ctacggtttc cgtagcaccg aaatccggcg ccgccttgct gtccgctgcc gccggcagcg
      661 gcggcgcggg cctcgcggcg aaagtgcagg ggccgcaggc agtgcttgac ctgctgtcgt
      721 cgattcatct tgcgggcact gccgacgagg cgcgcacgcg agcggcatcg ctggcgccgg
      781 gcgaatcggt gatttccgcc gacggcgtct ggcgcgggcg cagctgggtg cgctatccgc
      841 gcagcggcga cggcgagagc ggcgtgatcg cgcgcgggca gctgctgcgc gaactgaaag
      901 tgcaggtcga gcagcagaca cagacgctgg ccacgcgcga gcaggcgctg cgcgagctgt
      961 cgcagcgcat gcaggcggcg gagaccgagc ggcgcgaggt cggcggtaag ttcgaccaga
     1021 gccgcggacg ccacgcgcag cggctggctt tccgccaggc gcaggccgtg cgcctggagc
     1081 aggcgcaggc gcgcgtgcag gcgctggtgc aggagctcga cgcgctcagg cagtcgcgcg
     1141 agcagcaggc cagcgagctc tccgaatcca agcagcgcct ggtcacgctg gaagccaccg
     1201 ccaacgagct gcgcaaccag cgcgccggca tgcagcaggc gctggcgacc aagcgcgacc
     1261 tcgtttcgcg cgcgcggcag gcgctgctgc tgtcgcagca ggcgcgcggg caggtgcaga
     1321 tcgatctcgc gtcgcacaag agctcgctgt cggcgctgga gcagtcgatc aaggatcgcg
     1381 gcgcccagcg cgaaacgctg gtggcgcggc gcagcgacga ggagcgcgcc gccggcgagc
     1441 tcgacacgcc gctggccgcg caggcgcacg aggtggagat cgcccgcgcg gcggtcaacc
     1501 aggcgcgcga ggcgctgcgc gcggcgcgcg agaaactcga agcggtcgaa gcgtcgttcg
     1561 ccggcatcct gcaggcggcg cgcgcggccg acgtggccaa ggacgctgcg caggagcgcg
     1621 tgcaggcggt gaagctggaa ttcgaaaacc tgcgcgcgcg gcgcgagggc tacaacgcgc
     1681 agatcgccga gaccggcttc gagcgcgagc cgctgttcgc cggcctcaag gacgacgcca
     1741 cgccggaagc gtgggaggaa aagctggcct cgatcctgcg ccgcatcgaa cgcctgggcc
     1801 cgatcaatct cgcggccatc caggaactgg aagaagcgca ggcgcgcgag aagtacatcg
     1861 gcgagcagta cgccgacctg accaacgcgc tgtccacgct ggaagaagcc atccgcaaga
     1921 tcgacgcgga aaccaaggag ctgttccgcc agaccttcga caaggtcgac accattttcc
     1981 gcgaagcctt cccgcgcctg ttcggcggcg gcgaggccta tctcgagctc accggcgacg
     2041 acctgctcga caccggcgtg cgcgtgatgg cgcgcccgcc cggcaagcgc aactcgtcga
     2101 tccagctgtt gtcaggtggt gagaaggcga tgaccgcggt ggcgctgctg ctggcgctgt
     2161 tccagctgac gccggcgccg ttctgcctga tggacgaagt ggacgcgccg ctggatgacg
     2221 ccaacgtggt gcgctttacc gacgttgtgc gcgaaatgtc gaaaaacgtg caattcatta
     2281 tcattacgca caacaagatc accatggagc tggcacagca gctgcacggc gtgaccatgc
     2341 aggagcctgg cgtatcgcgc ctggtgtccg tcgacgtggc gcaggcggtc gagctggccg
     2401 gcaagaacga ggtcaaagag gtttcctaaa tgacgatcct gcaatgggcc ttgctgatc
//
LOCUS       DASZZC010000599         9736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_954791,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000599 DASZZC010000000
VERSION     DASZZC010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954791"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="rodA"
                     /locus_tag="VHE37_12780"
     CDS             <1..741
                     /gene="rodA"
                     /locus_tag="VHE37_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HVT36454.1"
                     /translation="MTVAAFLHICRLPPRIITIVTALLLVGLPTVLVVMQPDLGTALL
                     IVTAGVFALFFAGLQWRWILGALAAVAAVAPAVWTKMHDYQRKRVLTFLDPESDPLGA
                     GYHITQSKIAIGSGGLFGKGWLNGTQAKLEFLPEAHTDFIFAVYSEELGLLGILLLLT
                     LYMAIVGRCLWIALRAQDTFQRLLASSLAMTFFIYVFINMGMVIGLMPVVGVPLPLVS
                     YGGTSAVTLLASFGMLMSIQTHRKLLAN"
     gene            741..1751
                     /locus_tag="VHE37_12785"
     CDS             741..1751
                     /locus_tag="VHE37_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVT36455.1"
                     /translation="MRTLALLLLSLALPAAADYHDHPRADLLRQNLKDNYGFSPEELA
                     QVDSALAQAQRIPKLVEAEQKAPEKTENWSQYSRRIDDDRVRGGLQLIAAHRETFTRA
                     EQEFGVPAPAIAAIMGVETRYGSLTGKTRVLDALATQGFDHPTRGDFFIGELAEFFAF
                     CREFGFMPDAPQGSYAGAMGDAQFMPSNYRRLALDYDHDGRTDLWSIDDAIGSIAHYF
                     VAYKPELGWTRGAPLVVPAALAGRLPDDFPRNGKAPTHTVAQIEAAGVRPWITLPPGQ
                     PAGLVELPLDDGGKEYWIALPNFYSVMTYNPRTFYAMAVSVLAQRIEQARDAEYAEAG
                     SR"
     gene            1748..2644
                     /locus_tag="VHE37_12790"
     CDS             1748..2644
                     /locus_tag="VHE37_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVT36456.1"
                     /translation="MKARLLALCTLLALAGCANFGPEKSGGGHKPISGPYEMDHDAAP
                     LPESIPPDVANVPDAVPQVEPLSKSGNPETYEALGQTYHVQKSADGFHQRGYASWYGR
                     KFHGHRTASGESYDMFAMTAAHKTLPLPTYVRVHRVGTDRSVIVKVNDRGPFHSRRII
                     DLSYAAAAKLGIVDDGEALVEIETVVPTLDNAHPVAVASAAPVAIATPAPAAAPAPVA
                     VAPRKPGWLQVAVYTDPINAVTLREDLTTMGIDNIAIRSSVIGDASLHRVLVGPYADP
                     EACADLRARIIARGLAADWVSE"
     gene            2727..3869
                     /locus_tag="VHE37_12795"
     CDS             2727..3869
                     /locus_tag="VHE37_12795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVT36457.1"
                     /translation="MKHIFPLLALAVLSFNTQAANIPDPPALDVKSYALIDFNSGQLL
                     AGKEPDARVEPASITKVMTVYIAFDELKQGRLHLDDQVLISEKAWREGKDSSESRMFV
                     DVGSRVRLEDILRGIIIQSGNDATVALAEHIAGSEDTFAEMMNQYAKKLGLTNTHFAD
                     ASGMPNPQHYSTAHDLTLLGRALIRDFPEQYKIFSEREFVFHNIRQGNRNLLLDMDPT
                     VDGIKTGHTEAAGYCLLGSAVRDGRRLVSAVMGDRSTKERANSTRALLGYGFRFFEDE
                     QLYGPQQPVASNYVWKGPGNEVPLGVMQPVALTLPRGAKPSLKIEPKIDAKIVAPVAA
                     GQRVGTVTVTLDGKLLRQDPLVALQAVGEGNLWQRTVDSVRLLIGK"
     gene            3875..4537
                     /gene="lipB"
                     /locus_tag="VHE37_12800"
     CDS             3875..4537
                     /gene="lipB"
                     /locus_tag="VHE37_12800"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HVT36458.1"
                     /translation="MQEHAKRAPAMDRMQAPVIKHLGRRDYAEVFAQMRTHTDARGDD
                     TPDELWFVEHPPVYTQGQAGKAEHVLAPGDIPVIQADRGGQVTYHGPGQIVVYFLIDL
                     HRIGYGVRTLVTRLEQAIVALLAQYGIAAQARADAPGVYVGASKIASLGLRVRRGRTY
                     HGIALNVDMDLEPFSRINPCGYQGLRMTQLRELGAPTDLAQVSRDLQMHLLHSLGYEK
                     SA"
     gene            4731..5552
                     /locus_tag="VHE37_12805"
     CDS             4731..5552
                     /locus_tag="VHE37_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT36459.1"
                     /translation="MTTTESPDGPLVRVRGLRFTHGARVIYDSVDVDVPRGKVTALMG
                     PSGTGKTTLLRIIGGQWRPRAGAVEFDGVNVHTQSRAQLFEMRKRMGMLFQNGALLTD
                     LSVFENVAFPLREHTRLPEPMIRDLVLMKLNAVGLRGARDLYPEQLSGGMARRVALAR
                     AIALDPQLVMYDEPFTGQDPISLGVLAKLIRELNDALGLTSIVVSHDVEEVLSIADYC
                     YVVSNGRVLDRGTPAQIRASESEWVQQFLQGKADGPVPFHYKAPPYARDLMGGAP"
     gene            5549..6376
                     /gene="mlaE"
                     /locus_tag="VHE37_12810"
     CDS             5549..6376
                     /gene="mlaE"
                     /locus_tag="VHE37_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HVT36460.1"
                     /translation="MTTPPRIEKVLERRRGERGFLADAITMVANFMQGLGRAALFLWH
                     MLLGVPAALARPHLVIQQMYMIGVLSLIIIVLSGSFIGMVMALQGYRALSLVGAKDAL
                     GIGLGLAILREFGPVVTALLFAGRAGSALAAEIGLMRATDQLSGMEMMAVDPIKRVIG
                     PRFGAGFLAMPLLGFIFCVMALGVAGGHLVGVDLLGVDAGAYWAGIKEGLHGEDIRDC
                     ATKSFTFGLTCTWIALYQGYHSPPTSEGVARATTATVVISSLAVLALDFVLTAFMFR"
     gene            6429..6965
                     /gene="mlaD"
                     /locus_tag="VHE37_12815"
     CDS             6429..6965
                     /gene="mlaD"
                     /locus_tag="VHE37_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HVT36461.1"
                     /translation="MQSRALEILVGIFVSLGVAAVLVLTLRVASLQDVGGSKGSYKVT
                     AKFENVGKLSVGNAVKIAGVTVGRVRAIGVDQNDFKAVVTMAIDNQYNSIPGDVGASV
                     LTAGLLGEQYIGLDGGGAEDPLKDGGTIKITQPALVLENLIGQLVSSFMNKKDDGLAN
                     AIGQLADALKSKDAGAHK"
     gene            6962..7582
                     /locus_tag="VHE37_12820"
     CDS             6962..7582
                     /locus_tag="VHE37_12820"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT36462.1"
                     /translation="MKTLLAVLLSLLAVTAQAAAPAPDAIVKNATEDLRKQISANHDQ
                     YKANLPAFYKVVDDKVVPHFDVPYIGKLVLARNWKTASDDQRTRFQTAFKDMLIKAYA
                     NALLDNYDAVDVAWAPLRMTADTTDVTVNSTLNRKAGPPIHISFLMHEADGDWKIYDV
                     LIENVSLVSNFRAQFTSEIKKTSLDDVIKRMESGELAAQPQQGGAK"
     gene            7579..7833
                     /locus_tag="VHE37_12825"
     CDS             7579..7833
                     /locus_tag="VHE37_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT36463.1"
                     /translation="MSAAGTPSGELSFATARGLLEALGGADSIDLTAISRADSAGISL
                     LLELTRRAQARSRTLTLHNANAQVRSLARFFGVDSLLEFR"
     gene            7845..8555
                     /locus_tag="VHE37_12830"
     CDS             7845..8555
                     /locus_tag="VHE37_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HVT36464.1"
                     /translation="MQAFLRGTAAALALTLCGCAHTPADDPADPLESMNRAVFAFNMK
                     ADQYVARPVAKGYVKVVPDFARTGINNFFNNLKYPTVIINDALQAKFLQGGEDLCRFV
                     VNSTVGVAGLLDPGTRIGLERHDEDFGQTLGKWGLGQGWYLMLPLLGPSSNRDLVGRA
                     GDYATSVTTWVNTSTGETIAIDGVDIIDTRAQLLSADSILDQQFDKYIFVRTAYLQHR
                     QALVYDGHPPKEDYDIGE"
     gene            complement(8703..>9736)
                     /locus_tag="VHE37_12835"
     CDS             complement(8703..>9736)
                     /locus_tag="VHE37_12835"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HVT36465.1"
                     /translation="ASLAIHDLGAPSADDQLRFSSAGREIYARARNELQKIWSETSYR
                     MARLRDNPECADEEFASVGADDDPGLSVKLSFEPAAPAMLADRPRVAILREQGVNGQS
                     EMAYAFLSAGFEPVDVHMSDVIEGRVRLAQFAGVVACGGFSYGDVLGAGQGWAKTILF
                     NARARDEFAGFLARQDRFALGVCNGCQMFAALQEIVPGAANWPMFRRNRSEQFEARWG
                     MVELLPSKSLFFDGMAGSRLPIAIAHGEGRAEFARAAQLDALVAGEQVAARFIDNHGN
                     VATRYPQNPNGSPGGITAICNDDGRVTILMPHAERTIAGATGSCWGQRWQQHTPWMQM
                     FHNARRWVS"
BASE COUNT         1629 a         3387 c         3168 g         1552 t
ORIGIN      
        1 atgacggtgg cggccttcct gcacatctgc cggctgccgc cgcggatcat caccatcgtt
       61 accgcgctgc tgctggtggg cctgcccacc gtgctggtgg tgatgcaacc ggacctgggc
      121 acggcgctgc tgatcgtcac cgccggcgtg ttcgcgctgt ttttcgccgg cctgcagtgg
      181 cgctggatcc tcggcgcgct ggccgcggtg gccgcggtgg cgccggcggt gtggaccaag
      241 atgcacgact accagcgcaa gcgtgtgctc accttcctcg acccggaaag cgatccactg
      301 ggcgccggct accacatcac gcagagcaag atcgccatcg gcagcggtgg cctgttcggc
      361 aagggctggc tcaacggcac gcaggcgaaa ctggaatttc tccccgaagc gcacaccgat
      421 ttcatcttcg ccgtgtactc cgaggaactt ggactgctgg gcatcctgct gctgctcacg
      481 ctgtacatgg ccatcgtcgg ccgctgcctg tggatcgcgc tgcgcgcgca ggacaccttc
      541 cagcgcctgc tggcctcgag cctggccatg accttcttca tctacgtgtt catcaacatg
      601 ggcatggtca tcgggctgat gcccgtggtc ggcgtgccgc tgccgctggt gtcctacggc
      661 ggcacctccg ccgtgaccct gctggccagc ttcggcatgc tcatgtcgat ccagacccac
      721 cgcaagctgc tggccaactg atgcgcacgc tggcgctgct gctgttgagc ctcgcactgc
      781 cggcagcggc cgattaccac gaccatccgc gcgcggacct gctgcgccag aacctcaagg
      841 acaactacgg cttctcgccg gaggaactgg cgcaggtcga ctctgcgctg gcgcaggcgc
      901 agcgcatccc caagctggtg gaggccgagc agaaggcgcc ggagaagacc gagaactggt
      961 cgcagtacag ccgccgcatc gacgacgacc gcgtgcgcgg cggcctgcag ctgatcgcgg
     1021 cgcaccgcga aaccttcacc cgcgccgagc aggaattcgg cgtgcccgcc cccgccatcg
     1081 ccgccatcat gggcgtggag acgcgctacg gttcgctcac cggcaagacg cgcgtgctcg
     1141 acgcgctggc gacgcagggt ttcgatcacc ccacgcgcgg cgatttcttc atcggcgagc
     1201 tggcggaatt ctttgccttc tgccgcgagt ttggcttcat gcctgacgcg ccgcagggtt
     1261 cttacgccgg tgcgatgggc gacgcgcagt tcatgcccag caactaccgc cggctggcgc
     1321 tggattacga ccacgacggc cgcaccgacc tgtggagcat cgacgacgcc atcggctcca
     1381 tcgcgcacta cttcgtcgcc tacaagcccg aactgggctg gacgcgaggc gcgccgctgg
     1441 tggtgccggc cgcgctcgcg ggcaggctgc cggatgactt tcctcgcaac ggcaaggcgc
     1501 ccacgcacac cgtggcgcag atcgaagccg ccggcgtgcg cccgtggatc acgctgccgc
     1561 cgggccagcc cgccggcctg gttgaactgc cgctggatga cggcggcaag gaatactgga
     1621 tcgcgctgcc caacttctac agcgtgatga cctacaaccc acgcaccttc tacgccatgg
     1681 cggtgagcgt gctggcgcag cgcatcgagc aggcgcgcga cgccgaatac gccgaggccg
     1741 gctccagatg aaggcaaggc tgctggcgct gtgcaccctg ctcgcgctgg ccggctgcgc
     1801 caacttcgga ccggaaaaat ccggcggcgg ccacaagccg atctccggtc cgtacgagat
     1861 ggaccacgac gccgcgccgc tgcccgagag catcccgccc gacgtggcca acgtgcccga
     1921 cgccgtgccg caggtcgagc cgctgtcgaa gtccggcaac ccggaaacct acgaggcgct
     1981 gggccagacc taccacgtgc agaaatcggc cgacggtttt caccagaggg gttacgcctc
     2041 ctggtacggg cgcaagttcc acggccaccg caccgccagc ggcgagtcct acgacatgtt
     2101 cgccatgacc gccgcgcaca agacgctgcc gctgcccacc tacgtgcgcg tgcatcgcgt
     2161 gggcaccgac cgcagcgtga tcgtgaaggt gaatgaccgc ggcccgtttc attcgcggcg
     2221 catcatcgac ctgtcgtacg cggcggcggc caagctcggc atcgtcgacg acggcgaggc
     2281 gttggtggaa atcgaaaccg tggtgccgac gctcgacaac gcgcacccgg ttgcggtggc
     2341 cagcgccgcg cccgttgcca tcgccacgcc cgcgcctgca gctgccccag cgccggtggc
     2401 cgtcgcgccg cgcaagcccg gctggctgca ggtggcggtg tacaccgacc cgatcaacgc
     2461 ggtcacgctg cgcgaagacc tcaccaccat gggcatcgac aacatcgcca tccgcagcag
     2521 cgtgatcggc gacgcctcgc tgcaccgcgt gctggtcggc ccctatgcgg acccggaagc
     2581 gtgcgcggac ctgcgcgcca ggatcatcgc gcgcgggctg gcggcggact gggtaagcga
     2641 atagttgcgc agccggcggc agcgcccgtg taaaatccgc gcccttcgcg caaacgcttt
     2701 tcttccccgc aggatgcagg ttgacgatga aacatatttt tccgctgctg gccctggccg
     2761 tgctgtcttt caatacgcag gccgcgaaca ttccggaccc gccggcgctg gacgtgaaga
     2821 gctacgcgct gatcgatttc aacagcggcc agctgctggc cggcaaggag cccgatgcac
     2881 gggtggagcc ggccagcatc accaaggtga tgacggtgta catcgccttc gatgagctca
     2941 agcagggccg cctgcatctc gatgaccagg tgctgatcag cgagaaggcc tggcgcgagg
     3001 gcaaggactc gtcggagtcg cgcatgttcg tcgatgtcgg cagccgcgtg cggctggagg
     3061 acatcctgcg cggcatcatc atccagtcgg gcaatgacgc caccgtggcg ctggccgagc
     3121 acatcgccgg cagcgaagac accttcgccg agatgatgaa ccagtacgcc aagaagctcg
     3181 gcctgaccaa cacgcatttc gccgatgcct cgggcatgcc caacccgcag cactacagca
     3241 cggcacatga cctgaccctg ctcggccgcg cgctgatccg cgattttccg gagcagtaca
     3301 agatcttctc cgagcgcgaa ttcgtgttcc acaacatccg ccagggcaac cgcaacctgc
     3361 tgctcgacat ggacccgacg gtcgacggca tcaagaccgg ccacaccgag gcggcgggct
     3421 actgcctgct cggctcggcc gtccgcgacg gccgccggct ggtttcggcg gtgatgggcg
     3481 accgcagcac caaggagcgc gccaacagca cgcgcgcgct gctcggttac ggcttccgct
     3541 tcttcgagga cgagcagctc tatggcccgc agcagccggt ggccagcaac tacgtgtgga
     3601 aaggcccggg caacgaagtg ccgctgggcg tgatgcagcc ggtggcgctg acgctgccgc
     3661 gcggcgcgaa gccctcgctg aagatcgagc cgaagatcga cgccaagatc gtggcgccgg
     3721 tggccgccgg ccagcgcgtg ggtacggtca cggtgacgct cgatggcaag ctgctgcgcc
     3781 aggacccgct ggtggcgctg caggcggtgg gcgaaggcaa cctgtggcag cgcacggtgg
     3841 attcggtgcg gctgctgatc gggaagtgac cgcgatgcag gagcacgcaa agcgcgctcc
     3901 tgcaatggac cgcatgcagg cgcccgtcat caagcatctg ggccgccgcg actacgccga
     3961 agtcttcgcg cagatgcgca cgcacaccga cgcgcgtggc gacgacacgc cggacgagct
     4021 ctggttcgtc gagcatccgc cggtgtacac gcagggccag gccggcaagg ccgaacacgt
     4081 gctcgctccc ggcgacatcc cggtgatcca ggccgaccgc ggcggccagg tgacctacca
     4141 cggccccggc cagatcgtgg tgtatttcct catcgacctg caccgcatcg gctacggcgt
     4201 gcgcacgctg gtcacgcgcc tggagcaggc catcgtcgcg ctgctggcgc agtacggcat
     4261 cgcggcgcaa gcgcgcgcgg acgcgccggg cgtgtacgtg ggcgcaagca agattgcctc
     4321 gctcggcctg cgcgtgcggc gcgggcgcac ctaccacggc atcgcgctca acgtggacat
     4381 ggacctggag ccgttctcgc gcatcaaccc ctgcggctac cagggactgc gcatgacgca
     4441 gctgcgcgag ctcggcgcgc ccaccgacct ggcgcaggtg tcgcgcgacc tgcagatgca
     4501 cctgctgcac agcctgggct acgaaaaatc tgcctgaccg cactgccgtt ttgattgtta
     4561 ttaattatct tataaatatt aatttatgtt ataattagta acagatggaa tgccggtacg
     4621 gtgggcacga tttacacgcc gcatctgctg cagcgccgcc cgcgtattgg ctatcatgcg
     4681 ccgcttcata cggttcgccg tgtggcagct ggcgcaccca cataccaacc atgacgacga
     4741 ccgaatcacc agacggcccc ctcgtccgcg ttcgcgggct gcgcttcacc catggcgcgc
     4801 gcgtgatcta cgacagcgtc gatgtcgacg tgccgcgcgg caaggtgacg gcgctgatgg
     4861 gcccgtccgg caccggcaag accaccctgc tgcgcatcat cggcggccag tggcggccgc
     4921 gcgccggcgc ggtggaattc gacggcgtca acgtgcacac ccagtcgcgc gcccagctgt
     4981 tcgagatgcg caagcgcatg ggcatgctgt tccagaacgg cgcgctgctc accgaccttt
     5041 cggttttcga gaatgtcgcc ttcccgctgc gcgagcacac gcgcctgccc gagccgatga
     5101 tccgcgacct ggtgctgatg aagctcaatg ccgtgggcct gcgcggcgcg cgcgacctct
     5161 acccggagca gctgtccggc ggcatggcgc ggcgcgtggc gctggcgcgc gccatcgcgc
     5221 tcgacccgca gctggtgatg tacgacgaac ccttcaccgg ccaggacccg atcagcctgg
     5281 gcgtgctggc caagctgatc cgcgagctca acgacgcgct gggcctgacc tcgatcgtgg
     5341 tgtcgcacga cgtggaggaa gtgctgtcca tcgccgacta ttgctacgtg gtgtccaacg
     5401 gccgcgtgct cgaccgcggc acgcccgcgc agatccgcgc cagcgaatca gagtgggtgc
     5461 agcagttcct gcagggcaag gccgacggcc cggtgccgtt ccactacaag gcgccgccct
     5521 acgcgcgcga cctgatggga ggcgcgccgt gaccacgccg ccgcgcatcg agaaagtgct
     5581 ggagcgccgc cgcggcgagc gcggcttcct ggccgacgcc atcaccatgg tggccaattt
     5641 catgcagggc ctgggccgcg ccgcgctgtt cctgtggcac atgctgctgg gcgtgccggc
     5701 ggcgctggcg cgcccgcacc tggtgatcca gcagatgtac atgatcggcg tgctgtcgct
     5761 gatcatcatc gtgctgtccg gctcgttcat cggcatggtg atggcgttgc agggctaccg
     5821 cgcgctgtcg ctggtcggcg cgaaggacgc gctgggcatc ggccttggcc tcgccatcct
     5881 gcgcgagttt ggcccggtgg tgacggcact gctgttcgcc ggccgcgccg gctcggcgct
     5941 ggccgcggaa attggtttga tgcgcgccac cgaccagctg tcgggcatgg agatgatggc
     6001 ggtggacccg atcaagcgcg tgatcggccc gcgcttcggc gccggcttcc tcgccatgcc
     6061 gctgctgggg ttcatcttct gcgtgatggc gctgggcgtg gccggcggcc acctggtcgg
     6121 cgtcgacctg ctgggcgtgg acgccggcgc ctactgggcc ggcatcaagg agggcctgca
     6181 cggcgaagac atccgcgact gcgccaccaa aagcttcacc ttcggcctga cctgcacctg
     6241 gatcgcgctg taccagggct accactcgcc gcccacttcg gaaggcgtgg cgcgcgcaac
     6301 caccgccacc gtggtcatct cctcgctggc ggtgctggcg ctggacttcg tactcaccgc
     6361 cttcatgttc cgctgacggt tcgcggaaca ttcaccccat gttcagtgac tcaccatagg
     6421 atcgagccat gcaatcacga gccctggaaa tcctggtcgg catcttcgtc agcctcggcg
     6481 tcgccgccgt gctggtgctg accctgcgcg tcgccagcct gcaggacgtc ggcggcagca
     6541 agggcagcta caaggtgacc gccaagttcg aaaacgtcgg caagctgtcg gtgggcaacg
     6601 cggtgaagat cgccggcgtt accgtcggtc gcgtgcgcgc cattggcgtg gaccagaacg
     6661 acttcaaggc ggtggtcacc atggccatcg acaaccaata caacagcatt ccgggcgatg
     6721 tcggcgccag cgtgctcacc gccggcctgc tgggcgagca gtacatcggc ctcgacggcg
     6781 gcggcgcgga agacccgctc aaggacggcg gcaccatcaa gatcacccag cccgcgctgg
     6841 tgctggaaaa cctcatcggc cagctggtca gttccttcat gaacaagaag gacgacggcc
     6901 tggccaacgc catcggccag ctggccgacg cactgaagtc caaggatgca ggagcccaca
     6961 aatgaagact cttcttgccg tgttgctctc gctgctcgcc gtcaccgcgc aggccgcggc
     7021 gccggcgccc gatgccatcg tcaagaacgc caccgaggac ctgcgcaagc agatctccgc
     7081 caaccacgat cagtacaagg ccaatctgcc ggccttctac aaggtggtgg atgacaaggt
     7141 ggtgccgcat ttcgacgtgc cctacatcgg caagctggtg ctggcgcgca actggaaaac
     7201 cgccagcgat gaccagcgca cgcgcttcca gaccgcgttc aaggacatgc tgatcaaggc
     7261 ctacgccaat gcgctgctcg acaactacga cgcggtcgac gtcgcctggg cgccgctgcg
     7321 catgaccgcc gacaccaccg acgtcaccgt caactccacg ctcaaccgca aggccggccc
     7381 gccgatccac atcagcttcc tgatgcacga agccgacggc gactggaaga tctacgacgt
     7441 gctgatcgag aacgtatcgc tggtgtccaa cttccgcgcg cagttcacgt ccgagatcaa
     7501 gaagaccagc ctggacgacg tgatcaagcg catggaatcc ggcgagctgg cggcgcagcc
     7561 gcagcagggc ggcgccaagt gagcgcagcc gggactcctt ccggcgagtt gtcgtttgcc
     7621 accgcgcgcg gcctgctcga agcgctgggc ggcgccgaca gcatcgacct caccgccatc
     7681 agccgcgccg acagcgccgg catctccttg ctgctcgaac tgacgcgccg cgcgcaggcg
     7741 cgcagccgca cgctgacgct gcacaacgcc aatgcgcagg tccgctccct cgcccgcttt
     7801 tttggtgtag attcgctgct cgaattccgc tgaaggatcg gctcatgcaa gcttttctgc
     7861 gcggcacggc cgccgcgctg gcgctcacgc tgtgcggctg cgcgcatacg ccggcggacg
     7921 atcccgccga tccgctcgaa tcgatgaacc gcgccgtctt cgccttcaac atgaaggccg
     7981 accagtacgt ggcgcgcccg gtggccaagg gctacgtgaa agtggtgcca gacttcgccc
     8041 gcaccggcat caacaatttc ttcaacaacc tgaagtaccc gacggtgatc atcaacgacg
     8101 cgctgcaggc caagttcctg cagggcggcg aagacctgtg ccgtttcgtg gtcaacagca
     8161 cggtgggcgt cgccggcctg ctcgacccgg gaaccaggat cggcctcgaa cgtcacgacg
     8221 aggatttcgg ccagaccctg ggcaaatggg gcctcggcca gggctggtac ctgatgctgc
     8281 cgctgctcgg gccttcctcc aaccgcgacc tggtcggccg cgctggcgac tacgccacca
     8341 gcgtcaccac ctgggtcaac accagcaccg gcgaaaccat cgccatcgac ggggtcgaca
     8401 tcatcgacac gcgcgcgcag ctgctgtcgg ccgacagcat cctcgaccag cagttcgaca
     8461 agtacatctt cgtgcgcacc gcctacctgc agcaccggca ggcgctggtg tatgacggcc
     8521 atccgccgaa agaggattac gacatcggcg agtgattcaa gctgctgcgc tcggcatctt
     8581 gcgcgaaggc ggcgctcgca attctcacct gctccagcat gctccggttg ctgcactccg
     8641 tcctcgcgca acctgccttc gctcgcgacg cctgaatcgt cctgcgagtt tcaacgtggc
     8701 agtcaggaga cccaacgccg cgcgttgtgg aacatctgca tccacggcgt gtgctgctgc
     8761 cagcgctggc cccagcatga gcccgtcgcg ccggcgatgg tgcgctcggc atgcggcatc
     8821 aggatggtga cgcggccgtc gtcgttgcag atggcggtga tgccgccggg cgagccgttg
     8881 ggattctgcg ggtagcgcgt cgccacgttg ccgtggttgt cgatgaagcg cgcggccacc
     8941 tgctcgcccg cgaccagcgc gtcgagctgc gcggcgcgcg caaactcggc gcggccttcg
     9001 ccgtgggcga tggcgatggg caggcgtgag ccggccatgc cgtcgaagaa cagcgatttg
     9061 ctcggcagca gctcgaccat gccccagcgc gcctcgaact gctcgctgcg gttgcgccgg
     9121 aacatcggcc agtttgccgc gcccggcacg atctcctgca gcgcggcaaa catctggcag
     9181 ccgttgcaca cgcccagcgc aaagcggtcc tgccgcgcga ggaacccggc gaactcgtca
     9241 cgcgcccgcg cgttgaacag gatggtcttc gcccagccct ggcccgcgcc gagcacgtcg
     9301 ccgtaggaga acccgccgca ggcgaccacg ccggcaaact gcgccagccg cacgcggccc
     9361 tcgatcacgt cgctcatgtg cacgtccacc ggctcgaagc cggcggagag gaacgcgtag
     9421 gccatctcgc tctggccgtt gacgccctgc tcgcgcagga tggcgacgcg cgggcggtcc
     9481 gccagcatgg ccggcgcggc gggctcaaaa gaaagcttta ccgacaggcc cggatcatcg
     9541 tcggcgccca cggaggcgaa ttcctcgtcg gcgcattccg ggttgtcgcg cagccgcgcc
     9601 atgcggtaac tggtctcgct ccagatcttc tgcagctcgt tgcgcgcgcg cgcatagatt
     9661 tcgcggccgg cgctggagaa gcgcagctgg tcgtcggcac tgggcgcgcc gaggtcgtgg
     9721 atggcgagac tcgcat
//
LOCUS       DASZZC010000600         9623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_954912,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000600 DASZZC010000000
VERSION     DASZZC010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954912"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..448)
                     /locus_tag="VHE37_12840"
     CDS             complement(14..448)
                     /locus_tag="VHE37_12840"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT36466.1"
                     /translation="MAPELAATDAASYKFWITERIRFADLDVLGHVNNIAFTVYAESC
                     RAAFLRHIGFWTPQPGERQAVLAHIELDYRRELHYPGDVRVGLVVLHIGGSSVRVGLG
                     LFDGGHCAATAQAVLVRIERGSRRPTALNAEERASLAPYLRG"
     gene            complement(438..1316)
                     /locus_tag="VHE37_12845"
     CDS             complement(438..1316)
                     /locus_tag="VHE37_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36467.1"
                     /translation="MGICNNRSIIVTGAGAGLGRAHALALAAEGGSVVVNDINRAGAD
                     SVVAEILKSGGKAVASSDDITSMAGAEAIVAAGVQAFGDVHGVVNNAGILRDKMFASM
                     GEDDWDLVIKVHLKGHFCISSVLVKRWREQVKAGQKVDARIINTSSGAGLQGSVGQTN
                     YAAAKGGIASMTLVQAAELGRYGITANAIAPAARTGMTEKLFAEQMKKPADGSFDHYA
                     PENVSPLIVWLCSALSAGVTGKVFEVDGGKLSIASGWKTGSIVDKKARWNPSELTDVV
                     GRLVAEAPPAQKVYGT"
     gene            complement(1316..2467)
                     /locus_tag="VHE37_12850"
     CDS             complement(1316..2467)
                     /locus_tag="VHE37_12850"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVT36468.1"
                     /translation="MAEAFIVDALRTPTGKRKGSLAHVHGADLGAHALKALVARNNIP
                     AEDYDDVIFGCLDAIGPLAGNVARTCWLAAGLPLHVPGVTIDRQCGSSQQAVHFAAQA
                     VMSGTQDVVIAGGVQTMTQIPISSAMIAAQPLGFKDPFSGSKGWVERFGTQPVSQFVG
                     AQMIADKWKISRSDMENFALESHRRARVAQKEGRFNREIIEAWGLKTDETVRDTSLEQ
                     MAKLEPLAPGGTITAGVSSQTGDASSAVLVVSEKALKKYNLKPRARVHHISVYSDDPI
                     FMLTAPIPATKVALKKSGMKLEDIDLVEINEAFAPVVLSWLKETGYPHAQTNVNGGAI
                     ALSHPLGATGTKLMTTLLHELERTKKRYGLQTMCEGGGVANVTIIERLA"
     gene            complement(2652..3707)
                     /locus_tag="VHE37_12855"
     CDS             complement(2652..3707)
                     /locus_tag="VHE37_12855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT36469.1"
                     /translation="MDFTFDEQTLAFRDAIHKFLMVEAAPEFLREIWETKSGRSGELR
                     GKLAEQGLPGMSVPEAHGGLGLGDLDWVLVQQELGYYAIPDSLSDTAYLVVHLLKNLP
                     APSALAGEWLPKIAEGSARIALGHPLNPLVSDAEHAGLLLMWHDDEVHALQPAQADIK
                     AEPSIDMSRRLYRVQWHPSEGTRICKAGPGRALWAGVADRAAVALAAQLIGLAQRMLD
                     LGIDYAAQRKQFGKPVGSFQAVKHHLADVAVKIEFAKPVVYRAAYALAHGDARAPLYA
                     SHAKLAAGDAALLAARKSLQSHGAIGYTWEADLQMFMKRAWVLDAAWGTRAYHKSRVQ
                     DAILENGARIGPGTTFV"
     gene            complement(3825..4979)
                     /locus_tag="VHE37_12860"
     CDS             complement(3825..4979)
                     /locus_tag="VHE37_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT36470.1"
                     /translation="MKLEYTDAQKKFRAEIRAWLAAEVPKQPLPSFDTQEGFLMHRAW
                     EAKLYEGRWSAVTWPEELGGRGCDLTSWLIFEEEYWAAGAPLRVNQNGLFLLAPTLME
                     HGTEEQKKKFLPRMAQGLDIWAQGWSEPGAGSDMAAIRCKAVKDGDHYVINGQKTWST
                     RAVWADMLFGLFRSDPESQRHHGLTFILLPLNLEGISIRPIRQLNGLPGFAEIFFDNV
                     RVPVENRLGAEGQGWSVAMSTAGFERGLILRSPARFQKTAQNLVELYRRRRPEADADP
                     AIRDAVIKSWMDAEAYTTATYATVGRLARGGQIGAEASTNKIFWSELDLLMHETAMRI
                     LGARGELLHDKESAHWLDGFLFAQAGPIYAGTNEIQRNIIAERMLGLPRA"
     gene            complement(4976..5743)
                     /locus_tag="VHE37_12865"
     CDS             complement(4976..5743)
                     /locus_tag="VHE37_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HVT36471.1"
                     /translation="MSTGFTSTVTDGIAELVIDRPPVNALNDAGWHALADEIERLGRH
                     PEARVIVLRAEGRGFCAGVDIKELDQHPEKIVSVNAGNYRSFEAVHRNPLPVIVAVHG
                     FVLGGGIGLTGAADIVVASEDATFGVPEVDRGAMGGGAHLQRLFPVQKVRMMYFTGDI
                     ITAAEAYRLGAVERVVPRAELREAALVIARRIAAKSTAMIRLAKESLNGIEDGNLETK
                     YRWEQGFTLQAYANKDSAETRRAFVEKREAEFGNKKP"
     gene            complement(5740..6861)
                     /locus_tag="VHE37_12870"
     CDS             complement(5740..6861)
                     /locus_tag="VHE37_12870"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVT36472.1"
                     /translation="MSALDTPLTRLLGCRYPIVQTAMGWIADSKLVSATSNAGGFGFL
                     AAATMSTQQLKDAIAAVRAATPHNFGVNFHMFQPNALEIIEVVLENRDRVRAVSYGRG
                     PDAKTVKRFKDAGVLCMPTVGALKHAQKAIELGADIITVQGAEGGGHTGSVPTSLLLP
                     QVLDAVQVPVVAAGGFHNGRGLAAALAYGASGIAMGTRFLMTAESPVPRETLQKYIEV
                     NDPARIRASTAVDGLPQRMIDNPFLLRLESGGLLHRLYVALSSAWHWRAHTGMSIGHM
                     VRTFFAALREGDGAIQTMMAANAPVLIQRAMVQGKPDEGVLPSGQVAAVLTSLPTVQE
                     VIDGIVSEAQARIAALTATAAGGAHLSAVASPKTARYSS"
     gene            complement(6977..8158)
                     /locus_tag="VHE37_12875"
     CDS             complement(6977..8158)
                     /locus_tag="VHE37_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HVT36473.1"
                     /translation="MNRDIAILGAGMHPWGKWGRNFVEYGVVAARAALADSGVNWRDV
                     QFVSGAATIRNGYPGQVAGATFAQALGWQGAQVNTSYAACASGSQALAAARSKILSGE
                     CEVALVIGADTTPKGFLKPQPGDRPDDPDWVRFRVGITNPTYFALYARRRMALYGDTQ
                     ADFAAVKVKNAAHGFENANARYRKKFGHEEVAASAMVADPLRLMDICATSDGAAALIV
                     SSLDYAKKIGKGHAPRIAAISTVTPTFPSAIVEMPDIATDSAVTGKPHAFRAALPKKA
                     YEEAGISPKDISLAEVYDLSTALELDWIEDLQLCERGEAASLLRSGATTVGGRIPVNA
                     SGGLACFGEAVPAQAIAQVCELTFQLRGQAGARQVANARVGITANQGLFGHGSSVIVK
                     K"
     gene            complement(8155..8568)
                     /locus_tag="VHE37_12880"
     CDS             complement(8155..8568)
                     /locus_tag="VHE37_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVT36474.1"
                     /translation="MSMPVLESWFTLDAQQPHLLGSRCAACGTYYFPKAVSFCRNPDC
                     AGEKFEEVQLSRSGKLWSYTNAAYQPPEPFVQVDKDKFQPFAIAAVELEREKMIVLGP
                     VVNGVAVDALKVGMKMELKLEALADGKVTWKWAPA"
     gene            complement(8681..9472)
                     /locus_tag="VHE37_12885"
     CDS             complement(8681..9472)
                     /locus_tag="VHE37_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HVT36475.1"
                     /translation="MSEITLAELMIAAASEAWRNNGEVLASGITTIPRLAASLAKMTH
                     SPELLMTDSEAYLVAEPVPVGPRGDYVPKYEGYMSFERVFECVWGGRRHAMIGPTQID
                     RWGQSNLSAVGDYARPKSAVLGVRGLPGNSINHINSFFVPNHGPRVFVAGEVDMVSGV
                     GYKPERWGPGMKREYADIRIVVTDLCVLDFGGPDHAARVVSLHPGNSFESVQAATGFP
                     LLKAPEMKETALPSAEQLAIIRKLDPHNLRGAVLKNNPPAIKAAA"
BASE COUNT         1500 a         3273 c         3133 g         1717 t
ORIGIN      
        1 cccgcccggc gcgtcagccg cgcaggtacg gcgccaggct ggcacgctct tcggcgttca
       61 gcgccgtcgg cctgcggctg ccgcgctcga tgcgcaccag caccgcctgc gccgtcgccg
      121 cgcaatgccc gccgtcgaac aggcccaggc cgacgcgcac cgagctgccg ccgatgtgca
      181 gcaccaccag gcccacgcgc acgtcgccgg gatagtgcag ctcgcggcgg tagtccagct
      241 cgatatgcgc cagcaccgcc tggcgctcgc cgggctgcgg cgtccagaag ccgatatgcc
      301 gcaggaaggc cgcgcggcaa ctctcggcgt acacggtgaa cgcgatgttg ttgacgtggc
      361 ccagcacgtc gagatcggcg aagcggatgc gctcggtgat ccagaacttg tagctggccg
      421 cgtcggtggc ggcgagctca ggtgccatag accttctggg ccggcggcgc ctcggccacc
      481 agcctgccga ccacgtcggt cagttcggaa ggattccagc gcgccttctt gtcgacgatg
      541 ctgccggtct tccagccgct ggcgatggac agcttgccgc catcgacctc gaacaccttg
      601 ccggtgacgc cggccgacag cgcgctgcac agccacacga tcagcggtga gacgttctcc
      661 ggcgcgtagt gatcgaagct gccgtcggcc ggcttcttca tctgctcggc aaacagtttt
      721 tccgtcatgc cggtgcgcgc cgccggcgca atggcattcg cggtgatgcc gtagcggccc
      781 agttccgccg cctgcaccag cgtcatcgag gcgatgccgc ccttggccgc cgcgtagttg
      841 gtctgcccga ccgagccctg caggcccgca cccgaactgg tgttgatgat gcgcgcgtcg
      901 accttctgcc cggctttcac ctgctcgcgc cagcgcttga ccagcacgct gctgatgcag
      961 aaatggccct tgagatggac cttgatcacc aggtcccagt cgtcctcgcc catgctggcg
     1021 aacatcttgt cgcgcaggat gccggcgttg ttgaccacgc cgtgcacgtc gccgaaggcc
     1081 tgcacgccgg ccgcgacgat ggcctcggcg cccgccatgc tggtgatgtc atcgctgctg
     1141 gccacggcct tgccgccgga cttgaggatt tctgcaacga cgctgtccgc gcccgcgcgg
     1201 ttgatgtcgt tgaccaccac gctgccgcct tccgcggcaa gggccagcgc atgcgcgcga
     1261 ccaaggccgg cgccagcgcc ggtgacgatg atgctgcggt tgttgcaaat gcccattatg
     1321 cgagcctctc gatgatcgtc acgttggcca caccgccgcc ctcgcacatg gtctgcaggc
     1381 cgtagcgttt tttcgtgcgc tccagttcat gcagcagcgt ggtcatcagc ttcgtcccgg
     1441 tggcgcccag cggatgcgac agcgcaatgg cgccgccgtt gacgttggtc tgcgcgtgcg
     1501 ggtaaccggt ttccttgagc caggacagca ccaccggcgc gaaggcctcg ttgatctcca
     1561 ccaggtcgat gtcttccagc ttcatgccgg atttcttcag cgcgactttc gtcgcgggaa
     1621 tcggtgcggt gagcatgaag atcgggtcat ccgaatacac gctgatgtgg tgcacgcgcg
     1681 cgcgcggctt caggttgtac ttcttcagcg ccttctcgct gaccaccagc acggcggacg
     1741 acgcatcgcc ggtctgcgac gacacgcctg cggtaatcgt gccgccgggc gccagcggtt
     1801 cgagtttcgc catctgctcg agcgaggtgt cgcgcacggt ctcgtcggtc ttcaggcccc
     1861 aggcctcaat aatttctcga ttgaagcggc cttccttctg cgccacgcgc gcgcggcggt
     1921 gcgattccag cgcgaaattc tccatgtcgc tgcgcgaaat cttccatttg tccgcgatca
     1981 tctgcgcgcc gacgaactgc gacaccggct gcgtgccgaa acgctcgacc cagcccttcg
     2041 atcccgagaa cggatccttg aagcccagcg gctgcgccgc gatcatcgcc gacgagatcg
     2101 ggatctgcgt catggtctgc acgccgccgg cgatgaccac gtcctgcgtg ccgctcatca
     2161 ctgcctgcgc ggcgaagtgc acggcctgct gcgacgagcc gcactggcgg tcgatggtca
     2221 cccccggcac gtgcagcggc aggccggcgg ccagccagca ggtgcgcgcg acgttgccgg
     2281 ccagcgggcc gatggcgtcg aggcagccga agatgacgtc gtcgtaatcc tcggcgggaa
     2341 tgttgttgcg cgcgaccagc gccttcagcg cgtgcgcgcc caggtccgcg ccgtgcacgt
     2401 gcgccagcga gcccttgcgc ttgccggtgg gcgtgcgcag cgcgtcgacg atgaatgctt
     2461 ctgccatgtt caggctccgg gattcgggat ggaggattgg ggatagctaa gcgaagcttc
     2521 caccagcaac cgcaggcgct ttttgatgtt attaaatatc atgtatttta aatttaatta
     2581 aataattact aacggcataa tcccggtgcg gtcagcgtgt ttttcacccg tcccgaaccg
     2641 ccaatcccgt ctcacacgaa tgtcgtgccc ggcccgatgc gcgcaccgtt ttccaggatc
     2701 gcatcctgca cgcgcgactt gtgatacgcg cgcgtgcccc aggcggcatc gagcacccag
     2761 gcgcgcttca tgaacatctg caggtccgct tcccaggtgt agccgatggc gccgtgcgat
     2821 tgcaggctct tgcgcgcggc cagcagcgcg gcgtcgccgg cggcaagctt ggcatgcgag
     2881 gcatacagcg gcgcgcgcgc gtcgccgtgc gccagcgcgt aggcggcgcg gtagaccacc
     2941 ggcttggcga attcgatctt caccgcgacg tcggccaggt ggtgcttcac cgcctggaac
     3001 gaaccgaccg gcttgccgaa ctgcttgcgc tgcgcggcgt agtcgatgcc caggtcgagc
     3061 atgcgctgcg ccaggccgat cagctgcgcc gccagcgcca ccgcggcgcg gtcggccacg
     3121 ccggcccaca gcgcgcggcc ggggccggcc ttgcagatgc gcgtgccctc ggacggatgc
     3181 cactgcacgc ggtacaggcg gcgcgacatg tcgatcgacg gctccgcctt gatgtcggcc
     3241 tgcgccggct gcagcgcgtg cacctcgtcg tcatgccaca tcagcagcag gccggcgtgc
     3301 tcggcatcgg acaccagcgg gttgagcggg tggcccagcg cgatgcgcgc gctgccttcg
     3361 gcgatcttcg gcagccattc gccggccagc gccgacggcg ccggcaggtt tttcagcagg
     3421 tgcaccacca ggtaggcggt gtcggacagc gaatcgggaa tcgcgtagta gcccagctcc
     3481 tgctgcacca gcacccagtc gagatcgccc aggcccaggc cgccgtgcgc ctcgggcacc
     3541 gacatgccgg gcagcccctg ctcggccagc ttgccgcgca gctcgccgct gcggccgctc
     3601 ttggtttccc agatctcgcg caggaactcc ggcgccgctt ccaccatcag gaatttgtgg
     3661 atggcgtcgc ggaaggccag cgtctgttcg tcgaaggtga agtccatggt gctattccgt
     3721 catccccgcg aaggcgggga tccagttctt cttgccccca ccgcaaaaaa caaaactgga
     3781 ttcccgccta cgcgggaatg acagctcccc tttggtatgc gttttcatgc gcgcggcaat
     3841 ccgagcatgc gttccgcgat gatgtttctc tgtatctcat tggtgccggc gtagatcggc
     3901 ccggcctgcg cgaacaggaa gccgtcgagc cagtgcgcgg attccttgtc gtgcagcagt
     3961 tcgccgcgcg cgcccaggat gcgcatggcg gtctcgtgca tcagcaggtc cagctccgac
     4021 cagaagatct tgttggtgct ggcctcggcg ccgatctggc cgccgcgcgc caaccgtccc
     4081 accgtggcat acgtggcggt ggtgtaggcc tcggcatcca tccagctctt gatcaccgcg
     4141 tcgcggatgg ccggatcggc atcagcctcg gggcggcggc ggcggtacag ctcgaccagg
     4201 ttctgcgcag tcttctggaa gcgcgcaggc gaacgcagga tcaggccacg ttcgaagccg
     4261 gcggtggaca tggccacgct ccagccctgt ccttccgcac cgaggcggtt ttccaccggc
     4321 acgcgcacgt tgtcgaagaa gatctcggcg aagcccggca ggccgttgag ctggcggatc
     4381 gggcggatgc tgatgccttc gagattgagc ggcagcagga tgaaggtgag gccgtggtgg
     4441 cgttgcgatt ccgggtcgct gcggaacagg ccgaacagca tgtcggccca gaccgcgcgc
     4501 gtgctccagg tcttctggcc gttgatcacg tagtgatcgc cgtccttcac cgccttgcag
     4561 cggatcgcgg ccatgtcgga accggcgccc ggctcgctcc agccctgcgc ccagatgtcc
     4621 aggccctgcg ccatgcgcgg caggaatttc ttcttctgct cctcggtgcc gtgttccatc
     4681 agcgtcggcg ccagcaggaa caggccgttc tggttgacgc gcagcggcgc gccggcggcc
     4741 cagtattcct cttcgaagat cagccacgag gtcaggtcgc agccgcggcc gcccagttcc
     4801 tccggccagg tcaccgcgct ccagcggcct tcgtacagct tggcttccca ggcgcggtgc
     4861 atgagaaagc cttcctgggt gtcgaagctc ggcagcggct gcttcggcac ttccgccgcc
     4921 agccaggcgc ggatctcggc gcggaatttc ttctgcgcgt cggtgtattc cagtttcatg
     4981 gtttcttgtt cccgaactcc gcttcgcgtt tttccacgaa cgcacggcgg gtttctgctg
     5041 aatccttgtt ggcgtacgcc tgcagcgtga agccctgctc ccagcgatac ttcgtttcca
     5101 ggttgccgtc ctcgatgccg ttgagcgatt ccttggccag ccggatcatc gccgtgctct
     5161 tggcggcgat gcggcgggcg atgaccagcg cggcttcgcg cagttccgcg cgcggcacta
     5221 cccgctccac cgcgccgagc cggtaggcct ctgccgcggt gatgatgtcg ccggtgaaat
     5281 acatcatgcg caccttctgc accgggaaca ggcgctgcag gtgcgcgccg ccgcccatgg
     5341 cgccgcggtc cacttccggc acgccgaagg tggcgtcttc cgatgccacg acgatatcgg
     5401 ccgcgccggt caggccgatg ccgccgccga gcacaaagcc gtgtaccgcg acgatcaccg
     5461 gcagcgggtt gcggtgcacc gcttcgaagc tgcggtagtt gccagcattg accgagacga
     5521 tcttctcggg gtgctggtcc agttccttga tgtcgacgcc ggcgcagaaa ccgcgccctt
     5581 ccgcgcgcag cacgatcacg cgcgcctcgg gatgcctgcc aaggcgctcg atctcgtcgg
     5641 ccagcgcgtg ccagccggcg tcgttgagcg cgttgaccgg cgggcggtcg atcaccagct
     5701 cggcgatgcc atcggtgacg gtcgaagtga atcccgtgct catgagctgt atctcgcggt
     5761 cttgggtgag gccacggcgc tcaggtgcgc accgccggca gccgtggcgg tcagtgccgc
     5821 aatacgcgcc tgcgcctcgc tgacgatgcc atcgatcact tcctgcacgg tcggcagcga
     5881 ggtcagcacc gccgccacct ggccgctagg cagcacgccc tcgtcaggct tgccctgcac
     5941 catggcccgc tggatcagca ccggcgcgtt ggccgccatc atggtctgga tggcgccatc
     6001 gccctcacgc agcgcggcga agaaagtgcg caccatgtgg ccgatgctca tgccggtatg
     6061 ggcgcgccag tgccaggcgc tcgacagcgc cacgtacagg cgatgcagca ggccgccgct
     6121 ctccagcctg agcaggaaag gattgtcgat catgcgctgc ggcaggccgt cgaccgcggt
     6181 cgaggcgcgg atacgcgcgg gatcgttcac ctcgatgtat ttctgcaggg tctcgcgcgg
     6241 caccggcgat tccgccgtca tcaaaaagcg cgtgcccatc gcaatgccgc tggcaccata
     6301 cgccagtgcg gcggcgagtc cgcggccgtt gtggaatccg cccgcagcaa ccaccgggac
     6361 ttgcaccgcg tcgagcacct gcggcagcag cagcgatgtc ggcaccgagc cggtgtgccc
     6421 gccgccctcg gcaccctgca ccgtgatgat gtcggcgccg agctcaatgg ctttttgcgc
     6481 gtgcttgagc gcgcccaccg tgggcatgca cagcacgccg gcgtccttga aacgcttcac
     6541 cgtctttgca tccggcccgc gtccatacga cacagcgcgc acgcggtcgc ggttttccag
     6601 caccacctcg atgatctcca gcgcgttggg ctggaacatg tggaagttca cgccgaagtt
     6661 gtgcggcgtg gcggcacgca ccgcggcaat ggcatctttc agctgctgcg tcgacatggt
     6721 cgccgcggcg aggaaaccga agccgccggc attgctggtg gcggacacca gcttcgaatc
     6781 ggcgatccag cccatcgccg tctgcacgat gggatagcgg cagccaagca atcgggtcag
     6841 tggcgtatcg agcgcgctca tgacacgggg ccgtgagcgg gcgccagccc gcgagcgtgg
     6901 gtgcgggaat gagcgcatcg cgccgatagc gctcgcctta aagcggaaaa cgattctagc
     6961 cggggcgcgc tcatcgctat ttcttcacga tcaccgacga gccgtggccg aacagaccct
     7021 ggttcgcggt gatgccgacc ctggcatttg ccacctgccg cgcgccggcc tggccgcgca
     7081 gctggaaagt gagttcgcac acctgcgcga tggcctgcgc cggtaccgct tcgccaaagc
     7141 aggccaggcc gccggaggca ttgaccggga tacggccgcc gacggtggtc gcgccactgc
     7201 gcagcagcga ggcggcttcg ccgcgctcgc acagctgcag gtcttcaatc cagtccagct
     7261 ccagcgcggt cgacaggtca tagacctcgg ccaggctgat gtccttgggc gagatgcccg
     7321 cttcctcgta ggctttcttc ggcaatgccg cgcggaaggc gtgcggcttg ccggtgacgg
     7381 cggagtccgt cgcaatgtcc ggcatttcga cgatagctga cgggaaggtc ggcgtgaccg
     7441 tggaaatggc ggcgatgcgc ggcgcatgac ccttgccgat tttcttcgcg tagtcgagcg
     7501 aagacacgat cagcgccgcc gcgccgtcgg acgtggcaca gatatccatc agccgcaacg
     7561 ggtcggccac catcgccgac gcggcgactt cctcgtggcc gaatttcttg cggtagcgcg
     7621 cgttggcatt ctcgaagcca tgcgccgcgt tcttcacctt caccgcggcg aagtccgcct
     7681 gcgtgtcgcc gtacagggcc atgcgccggc gcgcatagag cgcgaagtaa gtcggattgg
     7741 tgatgccgac gcggaagcgc acccagtctg gatcgtcggg acggtcgccc ggctgcggct
     7801 tgagaaatcc tttcggcgtg gtgtcggcgc cgatgaccag cgcgacttca cattcaccgg
     7861 aaagaatctt ggagcgcgcg gcagccagcg cctgcgaacc ggaagcacag gccgcatacg
     7921 aggtgttgac ctgcgcgccc tgccagccca gcgcctgcgc aaatgtggcg cccgcgacct
     7981 ggccgggata gccgttgcgg atggtggcag cgccggagac gaactgcacg tcgcgccagt
     8041 tgacgccgga gtcggccagc gcggcgcgcg cggcgacgac gccgtactcc acgaagttcc
     8101 tgccccactt gccccacggg tgcatgcccg cgccaagaat cgcgatatca cggttcatgc
     8161 tggcgcccac ttccaggtca ccttgccatc ggcaagcgct tcgagcttca actccatctt
     8221 catgccgacc ttgagcgcgt cgacggcgac cccgttcacc accgggccca gcacgatcat
     8281 cttttcgcgt tccagctcca ccgcggcgat ggcgaagggc tggaacttgt ccttgtccac
     8341 ctgcacgaag ggctcgggcg gctggtaggc cgcgttggtg taggaccaca gcttgccgct
     8401 gcgcgacagc tgcacttcct cgaacttctc gccggcgcaa tccgggttgc ggcagaagct
     8461 caccgccttg gggaagtaat aggtgccgca ggcagcgcaa cgcgaaccga gcaggtgcgg
     8521 ctgctgcgcg tcgagcgtga accagctttc gaggaccggc atgctcatct ggaactcacc
     8581 gtcatccccg cgcaggcggg gatccagttt tggctcgaga gaacagcaac actggattcc
     8641 cgcctgcggg ggaatgacca gaacaagaaa gctcgcgcgt tcatgcggca gccttgatcg
     8701 cgggcgggtt gttcttcagc actgcgccgc gcaggttgtg cgggtcaagc ttgcggatga
     8761 tcgccagttg ctccgccgac ggcagcgccg tctccttcat ttccggcgcc ttcagcagcg
     8821 ggaaaccggt cgcggcctgc acggattcaa agctgttgcc gggatgcagt gagaccacac
     8881 gcgccgcgtg atccggcccg ccgaaatcca gcacgcagag atcggtgacg acgatgcgga
     8941 tgtccgcata ctcgcgcttc atgccggggc cccagcgctc aggcttgtag cccacgccgg
     9001 acaccatgtc gacctcgccg gccacgaaca cgcgcgggcc gtggttgggc acgaagaagg
     9061 agttgatgtg gttgatgctg ttgcccggca ggccgcgcac gcccagcacc gcggacttgg
     9121 gcctggcgta gtcaccgacg gcggaaagat tgctctggcc ccagcggtcg atctgcgtcg
     9181 gcccgatcat cgcgtggcgc ctgccgcccc acacgcactc gaacacgcgc tcgaacgaca
     9241 tgtaaccctc gtacttgggc acatagtcgc cgcgcggacc gaccggcacc ggctcggcca
     9301 cgagatacgc ctcgctgtcg gtcatcagca gttccggcga gtgcgtcatc ttggccagcg
     9361 atgcggccag gcgcggaatg gtggtgatgc cggaggccag cacttcgcca ttgttgcgcc
     9421 aggcttcgct ggcggcggcg atcatcagtt ctgccagggt gatttcgctc atgtcagaac
     9481 accggcagtt tcagcttgga gattttttcg aggccgccgg ccttggcgat gtatgcggct
     9541 tcgcccggtg caacgaattc ctcgacatat ttctgccagc cgttttcttc cgtggccgag
     9601 gcgttgtagc gcttcaggtg ttc
//
LOCUS       DASZZC010000601         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1073512,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000601 DASZZC010000000
VERSION     DASZZC010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073512"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..964
                     /locus_tag="VHE37_12890"
     CDS             137..964
                     /locus_tag="VHE37_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36476.1"
                     /translation="MERKKNWIPAFAGMAMLLFTGICWSTQAAESSTPTAAATGSSGE
                     SFDFFSNKPVESTQVVELPPEKSKWLTVGAPAALFGFFLFQVFTVWWLVPFKLSAVDI
                     NLHDFPTGVKRGIAMAIGLFGIAFCFGVSEIHYQLTLHGSTEAYFQQMSLGKLIAFTH
                     AHLFGFTTSFFIIGIPFSMQFNHLPAYQWLFPLGLAASLTDVASWWGLKYVSANFEYI
                     TYFCGFVFSSSYLVMLVGLLRVLLFPEVIWRTDKDRAQRLRAIAEKDLASRHHEGDY"
     gene            1036..1959
                     /locus_tag="VHE37_12895"
     CDS             1036..1959
                     /locus_tag="VHE37_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVT36477.1"
                     /translation="MKHGIFSMAALAALAASSVAQAGGYAGAGLGYSSFLSRNDQATL
                     AVAGAPTPVTEKIDGALSGTFYAGWRDEHYPVMAEVAYLDMGNHKFSLTAASGSQEDR
                     YGLKAINLSAGVIPWRHPNGSAVWVKAGYYNGDASDKFASSDGFNLQYSQKTAGLSAA
                     LGGELALAPGLALRLEYLALINARDYSDDDGRGRSTLGLATLGLTFNFGSRDGAHGSA
                     AHMSSGMTAAAAPVAAPVPVAAPAPIHGPALRAGTALRGRPTPDASAVATYAAETPVQ
                     VLSRAENNSGSWCYVKVGDVVGWVPDTQVRP"
     gene            2156..>4034
                     /locus_tag="VHE37_12900"
     CDS             2156..>4034
                     /locus_tag="VHE37_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36478.1"
                     /translation="MNYFVTGATGFIGKFLLERLLARDGATVYCLIRESSADKFAALA
                     ERYGDAGKKLVMLAGDVTTPGLISADDFKKLKGKVDHLFHLAAVYDMNMSDEQGDRIN
                     NEGTRNVVAFANDLGGKVRLHHVSSVAVAGNGFVGRFTEDMFDEGQPVSHPYYRTKFQ
                     SEAIVRNEAKVPFRIYRPGAVVGDSRTGEMDKIDGPYYFFKAIQKISYSVPKWLPLVG
                     IEGGRVPLAPVDYVAAAMDVLAHKDGLDGRCFHLIQSHSPTVGELLQTILEAAHGPEF
                     ARKFELPALPAPLKEIAGRVTSRIPRDVEKRISKVIGVPVSVLGYVSNRAVFDDKQAR
                     KELKGSDVQCPDIRDYVDKLWAYWELQLDVHAKVTRKAMTRIAGKVAMVTGASSGIGF
                     NVAKKLAAAGAKVILVARTREKLEETQKIIAKAGGEAHVYPCDLSDMASIDRCAQAVL
                     KDFGHVDILVNNAGRSIRRAVFESFERFHDFERTMQLNYFGAVRMIMNLLPSMAQRKS
                     GQIVNISSIGVLANAARFSAYVASKAALDAFSRCLSAEVKHKNIEITAIYMPLVRTPM
                     IAPTKMYDYVPTWSPEHAADVVIKAIVEKPKSVATPLGTAAQLSYALWPKINDYVLNR
                     GF"
BASE COUNT          736 a         1291 c         1315 g          692 t
ORIGIN      
        1 cggcgtcgat gcggcgatca tggcggcggt gaagaaggcg ggcaccatgg tttacgtgcg
       61 gctgaagcat gcataagtcc agccgctctt tttcccgtca ttcccgcgaa ggcgggaagc
      121 cagttctgtc gtgaacatgg aacggaagaa aaactggatt cccgccttcg cgggaatggc
      181 gatgttgctt ttcaccggca tctgctggtc aacgcaggct gcggaaagtt ctacgcccac
      241 ggcggctgca accggttcct ccggcgaaag cttcgacttc ttctccaaca agccggttga
      301 gagtacgcag gtggtcgaac tgccgccgga gaaatcgaag tggctgaccg tcggcgcgcc
      361 ggccgcgctg ttcggattct tcctgttcca ggtgttcacc gtgtggtggc tggtgccgtt
      421 caagctgtcg gcggtggaca tcaacctgca cgacttcccc accggggtga agcgcggcat
      481 cgccatggcc attgggttgt ttggcatcgc cttctgcttc ggcgtgtcgg agatccacta
      541 ccagctgacg ctgcacggca gcaccgaggc ctacttccag cagatgtcgc tgggcaagct
      601 catcgctttt acgcacgcgc atttgttcgg cttcaccacc agtttcttca tcatcggcat
      661 tccgttctcg atgcagttca accacctgcc ggcctaccag tggctgtttc ccctggggct
      721 ggcggcgtcg cttaccgacg tggcctcgtg gtggggcctg aaatatgtct cggccaactt
      781 cgagtacatc acctatttct gcggcttcgt cttctccagc tcctacctgg tgatgctggt
      841 cggcctgctg cgcgtgctgc tgttccccga ggtgatctgg cgcaccgaca aggatcgcgc
      901 gcagcggctg cgcgccatcg ccgagaaaga cctggcctcg cgccaccacg agggagatta
      961 ctgagcgtta tgatgcggcc cggccggcgc cgcgcgccgg aacggtaata aaagacgaac
     1021 gggaggagac acaccatgaa gcacggaatc ttttcgatgg ccgcgctcgc ggcgcttgcg
     1081 gcaagcagcg tcgcgcaggc gggtggctat gcgggcgcgg gcctgggcta ttcgagcttt
     1141 ctcagccgca acgaccaggc gacgctcgcc gtcgcgggcg cgccgacgcc ggtgacggag
     1201 aagatcgacg gcgcgctgtc cggcaccttc tatgcaggct ggcgcgacga gcactacccg
     1261 gtgatggcag aagtggcata cctggacatg ggcaaccaca agttctcgct gaccgcggct
     1321 tccggcagcc aggaagaccg ctacgggctg aaggccatca acctttccgc cggcgtgatc
     1381 ccgtggcgcc accccaacgg ctccgcggtc tgggtcaagg ccggctacta caacggcgat
     1441 gccagcgaca agttcgccag cagcgatggc ttcaacctgc agtactcgca gaagaccgcc
     1501 ggcctgagtg cggcgctggg cggtgagctg gcactcgcac ccgggcttgc gctgcgcctg
     1561 gagtacctgg cgctgatcaa tgcgcgcgac tattcggatg acgacggccg cggccgcagc
     1621 acgctgggac tggccacgct gggcctgacc ttcaacttcg gcagccgcga cggtgcgcat
     1681 ggctccgccg cgcacatgtc cagcggcatg accgccgccg cggcgcccgt tgccgcgccc
     1741 gtgccggtgg cggcacccgc gccgattcac ggcccggcgc tgcgtgccgg caccgcgctg
     1801 cgcggacgcc cgacgccgga tgccagcgcg gtggccacct acgccgcgga aacgccggtg
     1861 caggtgctgt cgcgtgcgga gaacaacagc ggcagctggt gctacgtgaa ggtcggcgac
     1921 gtggtgggct gggtgcccga tacgcaggtg cgcccgtaaa cgcgcaaaaa gatcgccacc
     1981 gcacccgacc tgggtgagtt ataaaatata gatataaata aaaataaata aataattagt
     2041 aacacaagaa catgaggggc ggtgccgttg cgcatcgccc cttttttctt ttcagcgcgg
     2101 caaacatcag taagattgcg ctcccacgag aacaacacgg gttggagtgc ggctcatgaa
     2161 ctacttcgtc acgggcgcca cgggattcat cggcaagttc ctgctcgaac gcctgctggc
     2221 gcgcgacggc gccacggtgt actgcctgat ccgcgaatcg tccgccgaca agttcgccgc
     2281 gctggcggag cgttacggcg acgccggaaa gaagctggtg atgctggcgg gcgacgtcac
     2341 cacgccgggg ctgatctccg ccgacgactt caagaagctc aagggcaagg tcgaccacct
     2401 gttccatctc gccgccgtct acgacatgaa catgtctgac gagcagggcg accgcatcaa
     2461 caacgagggc acgcgcaatg tcgtcgcctt cgccaacgat ctcggcggca aggtgcggct
     2521 gcaccacgtg tcgagcgtgg cggtggcggg caacggcttc gtcggccgct tcaccgagga
     2581 catgttcgac gagggccagc cggtcagcca cccgtactac cgcaccaagt tccagtcgga
     2641 ggccatcgtg cgcaacgagg ccaaggtgcc gttccgcatc taccgtccgg gcgcggtggt
     2701 gggcgactcg cgcaccggcg agatggacaa gatcgacggg ccctactact tcttcaaggc
     2761 gatccagaag atcagctaca gcgtgcccaa gtggctgccg ctggtcggca tcgagggcgg
     2821 ccgcgtgccg ctggcgccgg tggattacgt ggccgccgcc atggacgtgc tggcgcacaa
     2881 ggacggcctc gatggccgct gcttccacct gatccagtcg cactcgccca ccgtgggcga
     2941 actgctgcag accatcctcg aggccgcgca cgggccggag tttgcgcgca agttcgagtt
     3001 gcccgcgctg ccggcgccgc tgaaggagat cgccggccgc gtcacctcgc gcatcccgcg
     3061 cgacgtggag aagcgcatca gcaaggtcat cggcgtgccg gtgtcggtgc tgggttacgt
     3121 gagcaaccgc gcggtgttcg acgacaagca ggcgcgcaag gaattgaagg gcagcgacgt
     3181 gcaatgcccg gacatccgcg actacgtcga caagctgtgg gcctactggg aactgcagct
     3241 cgacgtgcat gccaaggtca cgcgcaaggc catgaccagg atcgccggca aggtggcgat
     3301 ggtcaccggc gcgtcgtcgg gcattggatt taacgtcgcg aaaaaactcg ccgccgcggg
     3361 cgccaaggtg atcctggtcg cgcgcacgcg cgagaagctg gaggagacgc agaagatcat
     3421 cgccaaggct ggcggcgagg cgcacgtgta tccgtgcgac ctgtccgaca tggcttccat
     3481 cgaccgctgc gcgcaggcgg tgctgaagga tttcggacac gtcgacatcc tggtgaacaa
     3541 tgccggccgc tcgatccgcc gcgcggtgtt cgagagcttc gagcgcttcc acgacttcga
     3601 gcgcaccatg cagctgaact acttcggcgc ggtgcgcatg atcatgaacc tgctgccgtc
     3661 gatggcgcag cgcaagagcg ggcagatcgt caacatcagc tcgatcggcg tgctggccaa
     3721 cgccgctcgc ttttccgcct acgtggcgag caaggccgcg ctcgatgcgt tcagccgttg
     3781 tctctccgcg gaagtgaagc acaagaacat cgagatcacg gcgatctaca tgccgctggt
     3841 gcgcacgccg atgatcgcgc cgaccaagat gtacgactac gtgcccacct ggtcgccgga
     3901 acacgcggcc gacgtggtga tcaaggcgat tgtcgagaaa cccaagtcgg tggcaacgcc
     3961 gctgggcacc gccgcgcagc tgtcgtacgc gctgtggccg aagatcaacg actacgtgct
     4021 caaccgcggc ttcc
//
LOCUS       DASZZC010000602         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_815560,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000602 DASZZC010000000
VERSION     DASZZC010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815560"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VHE37_12905"
     CDS             complement(<1..1555)
                     /locus_tag="VHE37_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HVT36479.1"
                     /translation="MNINYRALAGALLCALFALAGVSSAADNEDRNLTVPLYKSVALR
                     LDGSAKGMSVGNPGVADVVMLGSDTVQVVGKALGSTNLILWDAAHHAQTVYNVEVVHD
                     LATLKQRLHQMLPEDRIEVQSAQENIVLGGEAGNLARMQAALDLAQSFIGQCIESETN
                     SGHVGANAVAALPGAGKVRDCDKGKVINLMQVGGAQQVMIEIKVAEVSRDVVKRLDAN
                     LNIFNLSKHSGIGAVQGGATFPNALDPNGLQTPVLGALNGSASPIGPPVQAFQPNTPT
                     IDSSGLFMSYLTGNMFLEAVLDISRRNGLAKILSEPTLTTLNGQEAQFLSGGEFPVPV
                     PQGGATNAITIDFKEFGVGVKFLPVILDSGRINLKLNVSVSELSQSNNVAITPTSTNT
                     TFVIPSLTKRSAVSTVELSDGQTIGIAGLISDNVREFVDKLPGLGDIPGLGALFRSQE
                     FLSGQTELVIFVTPHLARPIAANKVRLPTDSFVPPGDLDFYLMGRLERPRSGSETRLR
                     TARGGPEGAH"
BASE COUNT          235 a          540 c          598 g          299 t
ORIGIN      
        1 agtgcgcgcc ctcgggcccg ccgcgcgcgg tgcgcaggcg cgtctcgctg ccgctgcgcg
       61 ggcgctccag gcggcccatc aggtagaagt cgagatcgcc cggcggcacg aagctgtcgg
      121 tgggcaggcg aaccttgttg gcggcgatgg gacgcgccag gtgcggggtg acgaagatca
      181 ccagctcggt ctgcccggac aggaattcct ggctgcggaa cagcgcgccc aggccgggaa
      241 tgtcgcccag gcccggcagc ttgtcgacga actcgcgcac gttgtcgctg atcaggccgg
      301 caatgcctat ggtttggccg tcgctgagct ccaccgtgct gaccgcgctg cgcttggtca
      361 gcgagggaat gacgaaggtg gtgttggtgc tggtcggcgt gatcgccacg ttgttcgact
      421 gcgacagctc gctgacgctg acgttgagct tgaggttgat gcgcccggaa tcgaggatca
      481 ccggcaggaa cttcacgccg acgccgaatt ccttgaagtc gatggtgatg gcgttggttg
      541 cgccgccctg cggcaccggc accgggaatt cgccgccgga caggaactgc gcctcctggc
      601 cgttgagcgt ggtcagcgtg ggctccgaca ggatcttggc caggccgttg cgccgcgaga
      661 tgtcgagcac cgcctccagg aacatgttgc cggtgaggta gctcatgaac agcccgctgc
      721 tgtcgatggt tggtgtgttc ggctggaacg cctgcaccgg cgggccgatc gggctggcgc
      781 tgccgttgag cgcaccgagc accggcgtct gcaggccgtt gggatcgagc gcgttgggga
      841 aggtcgcgcc gccctgcacc gcaccgatgc cgctgtgctt gctcaggttg aagatgttga
      901 ggttggcgtc gagccgcttg accacgtcgc gcgacacctc ggcgaccttg atctcgatca
      961 tcacctgctg cgcgccgccc acctgcatca ggttgatcac cttgcccttg tcgcagtcgc
     1021 gcaccttgcc ggcgccgggc agtgcggcga cggcattggc gccgacgtgg ccgctgttgg
     1081 tctcggactc gatgcactgg ccgatgaagc tctgcgccag gtcgagcgcc gcctgcatgc
     1141 gcgccaggtt gcccgcctcg ccgcccagta cgatgttctc ctgcgccgac tgcacctcga
     1201 tgcggtcctc cggcagcatc tggtgcaggc gctgcttgag cgtggccagg tcatgcacca
     1261 cctcgacgtt gtagaccgtc tgcgcgtggt gcgcggcgtc ccacaggatc aggttggtgg
     1321 agcccagcgc cttgccgacc acctgcacgg tgtcgctgcc gagcatgacc acgtcggcca
     1381 cgccggggtt gcccaccgac atgcccttgg cgctgccgtc gagcctgagc gccaccgact
     1441 tgtacagcgg cacggtgagg ttgcggtcct cgttatccgc cgccgacgag actccggcga
     1501 gcgcgaacag tgcgcagagc agcgcgccgg ccagcgcgcg atagttgatg ttcatgcggt
     1561 tgctcctggt cacggcttgg gcaggtcgcc aatgacctgc gtgccgcgga tgacggtgaa
     1621 ggacgggaca gccggttcgg tctgtgcgga ggtgcgcgga gtggcgcgcg gc
//
LOCUS       DASZZC010000603         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_623322,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000603 DASZZC010000000
VERSION     DASZZC010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623322"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..834)
                     /locus_tag="VHE37_12910"
     CDS             complement(37..834)
                     /locus_tag="VHE37_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36480.1"
                     /translation="MIAAHWRRRAAALLCAGLLAGCAAHAARAPRAVAPFSFARDTFA
                     YANELEWTYGPNGTHTVNADRPLDYSHRCFVMTSAARQFHLHARFAPEQPALAAAADY
                     QPLVQEVYRRSPRELEADSDPVVIPGYADLRAFSTAYGQLLRDESGSGFGSYFERGNW
                     RLVFPFTRDHQERTAGSLLGEIHAGEQPIVHLVRFPVITINHAVMLYGAVATGSEIAF
                     TVYDPNHPEKPMELLYDRATHTFNFAANNYFEGGRVDVYEIFRGALF"
     gene            complement(831..>1914)
                     /locus_tag="VHE37_12915"
     CDS             complement(831..>1914)
                     /locus_tag="VHE37_12915"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HVT36481.1"
                     /translation="DYRALMQQLFDFDRIRDWLKKGRTLRFDAMHAVTGPYAQRIFVE
                     QLGAPANSLCNAIPLEDFGGGHPDPNLIYAKPLVELMMGESAPDFGAASDGDGDRNMI
                     LGRGIFISPGDSLAMLAANLSGLPGYRGKLAGVARSMPTCRAVDHVAKALGIDCYETP
                     TGWKFFANLLDANRITLCGEESFGTSSSHVREKDGVWAVLAWLNVLAVTGLSVKDLAE
                     KHWMKYGRHYYARHDYDELPASQAEHVMQALAGELANLPGHSLGEWRVTEADEFSYQD
                     PVDGSVAVAQGLRVVCGDHARLVVRLSGTGTKGATLRIYLERYERGESALNWKTGEAL
                     APLAGAAERLLRIVEITGRRTPSVVT"
BASE COUNT          318 a          616 c          662 g          318 t
ORIGIN      
        1 ataacaaaaa aatgtgcatg cggtgggtga caaaaatcag aacagcgcgc cacggaagat
       61 ctcgtagacg tcgacgcggc ccccttcgaa ataattgttg gccgcaaagt tgaaggtgtg
      121 cgtggcgcgg tcgtacaaca gttccatcgg cttttccggg tgattggggt cgtacacggt
      181 gaaagcgatt tcgctgccag tggccaccgc gccatagagc atcaccgcgt ggttgatggt
      241 gatcaccggg aagcgcacca ggtgcacgat gggctgctcg ccggcgtgga tctccccgag
      301 cagcgaaccg gccgtgcgct cctggtgatc gcgcgtgaac ggaaacacca gccgccagtt
      361 gccgcgctcg aagtagctgc cgaagccgct gccgctttcg tcgcgcagca gttgcccgta
      421 ggccgtgctg aacgcgcgca ggtccgcgta accgggaatg accaccgggt cgctgtcggc
      481 ctcgagctcg cgcggtgagc ggcgatagac ctcctgaacc agcggctggt aatccgccgc
      541 ggctgccagc gccggctgct ccggcgcgaa tcgcgcgtgc aggtgaaact gccgcgcggc
      601 actggtcatg acgaagcagc gatgcgaata gtccagcggt cgatcggcgt tgaccgtgtg
      661 cgtgccgttc ggtccgtagg tccactcgag ctcgttggcg taggcgaagg tgtcgcgggc
      721 gaaggagaaa ggcgctacgg cgcggggagc gcgcgccgcg tgcgcggcac atccggcgag
      781 cagacctgcg caaagcaggg cagcggcgcg gcggcgccag tgcgccgcga tcaagtgacc
      841 acgctcggcg tgcgccgccc ggtgatttcc acgatgcgca gcagccgttc ggccgcgccg
      901 gccagcggcg ccagtgcttc gccggtcttc cagttcagcg ccgactcgcc gcgctcgtag
      961 cgttcgagat agatgcgcag cgtggcgccc ttggtgccgg tgcccgagag ccgcaccaca
     1021 aggcgtgcgt gatcgccgca gaccacgcgc aggccctgcg ccacggcgac gctgccgtcg
     1081 accggatcct ggtagctgaa ctcgtcggcc tcggtcacgc gccattcgcc aaggctgtgg
     1141 ccgggcaggt tggccagttc gccggccagc gcctgcatca cgtgctcggc ctgcgacgcc
     1201 ggcagttcgt cgtagtcgtg gcgcgcgtag tagtggcggc cgtatttcat ccagtgcttc
     1261 tccgccaggt ccttcactga caggccggtg acggccagca cgttgagcca ggccagcacc
     1321 gcccacacgc cgtccttttc gcgcacgtgc gaggaactgg tgccgaagct ttcctcgccg
     1381 cacagcgtga tgcggttggc gtcgagcagg ttggcgaaga atttccagcc ggtcggtgtc
     1441 tcgtagcagt cgatgcccag cgccttggcc acgtgatcca ctgcgcggca ggtcggcatg
     1501 gagcgcgcca cgccggcgag cttgccgcgg tagccgggca gtcccgaaag gtttgccgcc
     1561 agcatggcca gcgaatcgcc gggggagatg aagatgccgc ggcccaggat catgttgcgg
     1621 tcgccgtcgc cgtcgctggc ggcgccgaag tccggtgcgc tttcgcccat catcagttcg
     1681 accagcggtt tcgcgtagat caggttcgga tcgggatggc cgccgccgaa atcctccagc
     1741 ggaatggcat tgcacagcga attcgccggc gcgccgagct gctcgacaaa aatgcgctgc
     1801 gcgtaaggcc cggtcaccgc gtgcatggcg tcgaagcgca aagtgcggcc ctttttcagc
     1861 cagtcgcgga tgcggtcgaa gtcgaacagc tgctgcatca gcgcgcgata gtcg
//
LOCUS       DASZZC010000604         8078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_303884,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000604 DASZZC010000000
VERSION     DASZZC010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303884"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VHE37_12920"
     CDS             <1..392
                     /locus_tag="VHE37_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36482.1"
                     /translation="DEKTASARQLGKLIAQLRELLARSGIATPATEALLQRVDIRTEF
                     YGSGYALPAPGAAEAIARFTERQHGTRADLAYSGKALACFYQDLDRGHLPTPALYWHT
                     YSAQALPPGVTRAAREQVPEALRGYWS"
     gene            569..2506
                     /locus_tag="VHE37_12925"
     CDS             569..2506
                     /locus_tag="VHE37_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT36483.1"
                     /translation="MDHDRRSMRAALRACLILFCAALALPAQAGTVDLGNEVELEYKL
                     SVSYALAMRTRNADARLTDAPVEEFQSYLIDLNVGPGQPSQLFRFERQGLSQSMNSDD
                     GDRNFKKWALIHDRLSGLGELQLHWKDYGAVLSGSAFYDDVYHHHNDNDSAATLNLLG
                     PNGEYPDPQPTRFTSATKKYDGARARLLESYVYGSWWLGDESNLNLRFGRQLVAYGES
                     LFFSGIASAMGPADATKAFVPGAEIKDILLPVNQVSLNLAINNDLSLLGSYKLEFHPT
                     EIFPQGDYLSPSDVVGPGAYLVYGSANPLGGVSGCSGLLQNFHVLGLPVGVTPAAVET
                     ALCGLLGLAGQTLLNAPPYIYNFRGPDITPGAHGQYAVGLKYQVTSASNLGLYYLRYH
                     DTNPSVQLSVGYPVIGTIGGRPITTQVLNQATPVGYNVKYFDGIHLAGLTFSTVLGGF
                     NVAGELNYRDGVDTPVETIISGHVSPVFQRGRISQALVSALYVTNPDLFFDDLVLVNE
                     AQFLHVNGVDRLAPSLGIAPVGNGDTLFYDRDAYGFMSLVTPTRHNLFSGWDLSVPIA
                     FGWLIKGTPAMAGAFGPLYGEGDMRASLGLDLQYLQNLQFSAGYNWFFGDPAKTIKDS
                     TLAANPYADRDYATLSVKYNF"
     gene            2543..3358
                     /locus_tag="VHE37_12930"
     CDS             2543..3358
                     /locus_tag="VHE37_12930"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HVT36484.1"
                     /translation="MSAPEQDIAIAREWIARSDGVVIAAGAGMGVDSGLPDFRGREGF
                     WNHYPALKAAGIDFMRAASPATFRADAQLAWGFYGHRLNLYRRVAPHAGFALLRELAA
                     GKEHGAFVFTSNVDGHFQRAGFADERVHECHGSIHHLQCLANCRGRIWSAEHFQPQTD
                     DTRCRLLSPLPQCPDCGGLARPNILMFGDGGWESRRSDEQYERLARWLRGVRTPVAIE
                     LGAGTAVPSVRYFSESIGARVIRINPREAHIAPHLGIGLELGALQALPLLLGG"
     gene            3423..5441
                     /locus_tag="VHE37_12935"
     CDS             3423..5441
                     /locus_tag="VHE37_12935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVT36485.1"
                     /translation="MHQQLRHAARAALPFALILACGGAAAQDMSATVRPGDDFFDYVN
                     GDWLAKTGIPADRSSWGTGAQLTEDTNQRIRQLIEALAEDAKTGGEARKVADAYSAFM
                     DQKGIEAQGLTPLKPALDEIAAIKDRAALTRALGAGLRADVDPLNATNFYTENLFGIW
                     IAQGLSDPQHYRPYLLQGGLGMPDRAYYIDDNAHMAELRGKYRTHIERMFRLAGLDDP
                     AGRAARVFDLETQIAQVHATREESEDVQKGNNPWKLADFSVNAPGIDWPALFRAAGLG
                     AQRDFIAWHPDAIKGEAALVTLAPLDAWKDWLAFHRINHYADVLPEAIAQEKFDFEGR
                     TLSGTPEQQARWKRALAATDSELSDALGHLYVQRYFPAENKARIQAMVKNIAAAFDRR
                     IDHLDWMAPATRAQARAKLKTLYVGIGYPDRWRSYEGLDISRSDAYGNAVRAEQFHYR
                     QELAKLGRRVDRTEWCMAPQLVNAVNMPLQNALNFPAAILQPPYFDATASDAHNYGAI
                     GSIIGHEISHSFDDQGAQFDAQGRLRDWWTSADGEHFRKAAGALAAQYSAYKPFPDLA
                     INGQQTLSENLADLAGLNASFDAYRLSLAGKPADTAGQREFFIGYAQSWRNKLRDEAL
                     RRRVLTDGHSPARWRTYTVRNLDAWYAVFDVQPGQRLYLPPEQRVRVW"
     gene            complement(5452..>8078)
                     /locus_tag="VHE37_12940"
     CDS             complement(5452..>8078)
                     /locus_tag="VHE37_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVT36486.1"
                     /translation="REAQVAALMARRERKLALIVGPAGCGKTTLLIQWRKQMFLHGAS
                     IAWYNAGADDDDAQIAAYLVEALAQAGVAISAEALHVYTRSGGKAGKPLLAALVNDVQ
                     QHGREVYLVIDDFHYIASFGVLQLIDRWLALAPANFHLVLGTRVRPPLALAQLQAADQ
                     LTELRFAELRFDLDETRRFTAAQGLAQLTPTQVRTLHEITDGWAAGLQLLAFSLRKEP
                     APEAWFARQGQLSLSQEEALSGYLEKAAIEHLSEEELDFLTRISACRRLNRELCELLT
                     GNPRAGELLEKFETENLFLIPIETADPQPWYRFHRLFASFLNARLARHDEAERRKLHQ
                     LAAHWFAGKSLHVEAMRHAQAADDADFMVELIDRGARRMINSANFVQFLKWCDAVPRE
                     RLQSRLNASLCAGWAQLSCSRIDEFDRSIAAMEAHAGARRAEVATELALLKAYRAMRA
                     DDTAASLAMVEPMLPAPPASPFQALILYNIASLSLVYAGEFERAREVARLRHRHDIAS
                     RPEYPRPLVDVVAGFSHLVEGHIQLAIASLTPYISDALRTTAFGADAAGLFLSYLLEA
                     YYQSGELAQARDFLERHGDLIEAVGNADGLLYAWRVRARLQQIDGDVRGAADTLQRLE
                     EAGYRQRLDRLVAWSLYEQLRLALAQGNAAATPDLLRRLAALAARYRNEPPGARSGIV
                     LAALMARAEAAFAQEQDDVLVQIDAAEQAALTQRRGLQHTRLRLLRALALLRAGRKPQ
                     ALAAAREALKQAAALGQMRVLAEFGSAAVPLVELLQMAAPAAEIAQYLQSTMDAVAGP
                     RATENVAGMAPAPVLLSAREREVVELLAQALSIKSIARGLNLSPGTVKWHLKNIYGKL
                     GAVSREDALAKARGLRII"
BASE COUNT         1302 a         2886 c         2662 g         1228 t
ORIGIN      
        1 tggacgagaa aaccgccagc gcgcgccagc tcggcaagtt gatcgcgcag ctgcgcgagc
       61 tgctcgcgcg cagcggcatc gccacgcctg cgacggaagc gctgctccag cgcgtcgaca
      121 tccgcaccga gttctacggc agcggctacg cgctgccggc gcccggcgcc gccgaggcca
      181 tcgcgcgctt caccgagcgc cagcacggca cccgcgccga cctcgcctac agcggcaagg
      241 cgctcgcctg cttctaccag gatctcgacc gcggccatct gcccacaccc gcgctgtact
      301 ggcacaccta cagcgcgcag gcgctgccgc ccggcgtgac gcgcgccgcg cgcgagcagg
      361 tgcccgaagc gctgcgcggt tactggagct gagcgcacct gacaactgaa tcagctcttg
      421 gtctgcatca gaccacgcgc ggcgcgattt tgaaaagctg caaacggagg aggccggcgc
      481 tacgcgcatg cgcctaatct gaaaatcacc gacaactatt gcagcgtgcg tccgcggtgg
      541 gcggcacacg cgacggggac tttcagtgat ggaccacgac cgacgctcga tgcgcgccgc
      601 gctgcgcgcc tgcctgattc ttttttgcgc cgccctcgcg ctgccggcgc aagccggcac
      661 cgtcgacctc ggcaacgagg tggaactgga atacaagctg tcggtcagct acgcgctggc
      721 catgcgcacg cgcaatgccg acgcgcgcct gaccgacgcg cccgtcgagg aattccagtc
      781 gtacctgatc gacctcaacg tcggcccggg acagccatcg cagctgttcc gcttcgagcg
      841 ccagggtttg tcgcagtcga tgaactcgga cgacggcgac cgcaatttca aaaaatgggc
      901 gctgatccat gaccgcctca gcggcctggg cgagctgcag ctgcactgga aggattacgg
      961 cgcggtgctc agcggctcgg ccttctacga cgacgtctac caccaccaca acgacaacga
     1021 ttccgccgcc acgctcaacc tgctcgggcc caacggcgag tatcccgatc cgcagccgac
     1081 ccggttcacc agcgccacga aaaaatacga cggcgcacgc gcgcgcctgc tcgagtcgta
     1141 cgtgtatggc agctggtggc tgggcgacga gagcaatctc aacctgcgtt tcggccgcca
     1201 gctggtcgcg tacggcgaga gcctgttctt ctccggcatc gccagcgcga tggggccggc
     1261 cgatgccacc aaggccttcg tgcccggcgc cgagatcaag gacatcctgc tgccggtgaa
     1321 ccaggtgtcg ctcaacctcg ccatcaacaa cgacctcagc ctgctcggca gctacaagct
     1381 ggagttccat cctaccgaga ttttcccgca gggcgattac ctgtcgccgt cggacgtggt
     1441 cggccccggc gcgtacctgg tctatggctc ggccaacccg ctgggcggcg tctccggctg
     1501 ctcgggcctg ctgcagaact tccacgtgct cggcctgccg gtgggcgtca cgccggcggc
     1561 ggtggagacg gcgctgtgcg gcctgctcgg cctcgccggc cagaccctgc tcaatgcgcc
     1621 gccgtacatc tacaacttcc gcggacccga catcacgccc ggcgcgcacg gtcagtacgc
     1681 ggtgggcctg aaataccagg tgaccagcgc gagcaatctc ggcctgtact acctgcgcta
     1741 ccacgacacc aacccgtcgg tgcagctcag cgtgggctat cccgtcatcg gcaccatcgg
     1801 cggccggccg atcaccacgc aggtgctcaa ccaggcgacg ccggtcggct acaacgtgaa
     1861 gtatttcgat ggcatccacc tcgccgggct caccttttcc accgtgctcg gcggcttcaa
     1921 cgtggccggc gaactcaact accgcgacgg cgtcgacaca ccggtggaga ccatcatctc
     1981 cggccacgtc agcccggtgt tccagcgcgg ccgcatcagc caggcgctgg tgtcggcgct
     2041 gtacgtgacc aatcctgatt tattttttga cgacctggtg ctggtgaacg aagcgcagtt
     2101 cctgcacgtc aatggcgtcg accggctggc gccttcgctg ggcatcgcgc cggtcggcaa
     2161 cggtgacacg ctgttctacg accgcgacgc gtacggcttc atgagcctgg tcacgcccac
     2221 ccggcacaac ctgttttcgg gatgggacct gagcgtgccg attgccttcg gctggctgat
     2281 caagggcacg ccggccatgg ccggcgcctt cggcccgctc tacggcgaag gcgacatgcg
     2341 cgccagcctg ggcctcgacc tgcagtacct gcagaacctg caattctccg caggctacaa
     2401 ctggttcttc ggcgacccgg ccaagaccat caaggacagc acgctggccg ccaatcctta
     2461 cgcggaccgc gactacgcca cgctgagcgt gaagtacaac ttctgagcgg cgcggcgcgc
     2521 gcacctatac tggtgcgcat cgatgagtgc gcccgaacag gacatcgcca tcgcgcgcga
     2581 atggatcgcg cgcagcgacg gcgtcgtcat cgccgccggc gccggcatgg gcgtcgattc
     2641 gggcctgccg gatttccgcg ggcgcgaagg cttctggaat cactatccgg cgttgaaggc
     2701 agccggcatc gacttcatgc gcgcggcgag cccggccacc ttccgcgccg atgcgcaact
     2761 cgcctggggc ttctatggcc atcgtctcaa cctgtatcgc cgcgtggcgc cgcacgccgg
     2821 cttcgcgctg ctgcgcgaac ttgcggcggg caaggaacat ggcgcgttcg tcttcaccag
     2881 caatgtcgac ggccacttcc agcgtgccgg cttcgccgac gagcgcgtac acgaatgcca
     2941 cggctcgatc catcacctgc aatgcctcgc caactgccgg ggacgcatct ggagcgcaga
     3001 acactttcaa ccgcagaccg acgacacgcg ctgccgcctg ctgtcgccgc tgccgcaatg
     3061 tcctgactgc ggcggcctgg cgcggccgaa catcctgatg ttcggcgacg gcggctggga
     3121 atcgcgccgc agcgatgagc agtacgagcg cctggcccgc tggctgcgcg gcgtgcgcac
     3181 accggtggcg atcgagctcg gcgccggcac cgcggtgccg agcgtgcgtt atttttccga
     3241 atcgatcggc gccagggtca tccgcatcaa cccgcgcgaa gcgcacatag cgccgcatct
     3301 gggcatcggg ctggaacttg gcgcgctgca ggcgttgcca ttgctgctcg gcggctgagg
     3361 aaccaaaccg tgcggccacg gtcttgagcc tacgcggcat ccaacatgtc cgggggaacc
     3421 acatgcacca gcagctccgc cacgcggccc gcgccgccct gcccttcgcc ctgatcctgg
     3481 cctgcggcgg cgccgcggcg caggacatgt ccgccacggt gcggcccgga gacgacttct
     3541 tcgactacgt caacggcgac tggctggcga agaccgggat cccggccgac cgcagttcct
     3601 ggggcaccgg cgcccagctc accgaggaca ccaaccagcg catccgccag ctgatcgagg
     3661 cgctggccga agacgcgaaa accggaggcg aagcgcgcaa ggtggctgac gcctattccg
     3721 ccttcatgga ccagaagggc atcgaggcac agggactgac gccgctcaaa cccgcgctgg
     3781 acgaaattgc cgcgatcaag gatcgcgccg cgctcacgcg cgcgctgggt gccggcctgc
     3841 gtgccgatgt cgatccgctc aacgccacca acttctatac cgagaacctg ttcggcatct
     3901 ggatcgcgca ggggctgtcc gatccgcagc actaccggcc gtacctgctg cagggcggcc
     3961 tgggcatgcc cgaccgcgcc tactacatcg acgacaacgc gcacatggcc gagctgcgtg
     4021 gcaagtaccg cacgcacatc gaacgcatgt tccggctcgc cggtcttgac gatccggccg
     4081 gccgcgccgc gcgggtgttc gatctggaaa cgcagattgc gcaggtgcat gccacgcgcg
     4141 aggaatcgga ggacgtgcag aagggcaaca acccgtggaa gctggccgac ttcagcgtca
     4201 acgcgccggg catcgactgg cccgcgctgt tcagggccgc gggcctgggc gcgcagcgcg
     4261 acttcatcgc ctggcacccg gacgcgatca agggcgaagc cgcgctggtg acgctggctc
     4321 cgctcgacgc ctggaaagac tggctcgcct tccaccggat caaccattac gccgacgtgc
     4381 tgcccgaggc catcgcgcag gagaaattcg atttcgaggg gcgcacgctg tccggtacgc
     4441 ctgagcagca ggcgcgctgg aagcgcgcgc tggccgccac cgacagcgag ctgagcgatg
     4501 cgctcggcca tctctacgtg cagcgttatt tcccggcgga gaacaaggcg cgcatccagg
     4561 ccatggtgaa gaacatcgcc gccgccttcg accggcgcat cgaccatctc gactggatgg
     4621 cgccggccac gcgcgcgcag gccagggcca aattgaaaac cttgtacgtc ggcatcggtt
     4681 atcccgaccg ctggagatcg tacgaaggcc tggatatttc gcgcagcgac gcctacggca
     4741 acgcggtgcg cgcggagcag ttccactatc gtcaggagct ggccaagctc ggccgcaggg
     4801 tcgatcgcac cgagtggtgc atggcgccgc agctggtcaa cgcggtcaac atgccgctgc
     4861 agaacgcgct caactttccc gccgccatcc tgcagccgcc gtacttcgat gccaccgcca
     4921 gcgatgcgca caactatggc gccatcggct cgatcatcgg ccacgagatc agccacagct
     4981 tcgacgacca gggcgcgcag ttcgatgcgc agggccgttt gcgcgactgg tggacgtctg
     5041 cggatggcga acatttccgc aaggccgccg gcgcgctggc cgcgcaatac agcgcctaca
     5101 agccctttcc cgatctcgcc atcaacgggc agcagacgct gtccgagaac ctggccgacc
     5161 tcgcggggct caatgcctcg ttcgacgcct atcgcctgtc gctcgccggc aagccggccg
     5221 ataccgcggg ccagcgcgag ttcttcatcg gctatgcaca gagctggcgc aacaagctgc
     5281 gcgacgaggc gctgcgccgc cgcgtgctga ccgacggcca ctcgccggcg cgctggcgca
     5341 cgtacacggt tcgcaacctc gatgcctggt acgccgtgtt cgatgtgcag ccggggcagc
     5401 ggctgtacct gccgcccgag cagcgcgtgc gcgtgtggta acgcgcgcgg ctcagatgat
     5461 gcgcagcccg cgcgccttgg ccagcgcgtc ctcgcgcgac accgcgccga gcttgccgta
     5521 gatattcttc aggtgccatt tcaccgtgcc cggtgaaagg ttcagcccgc gcgcgatgct
     5581 cttgatcgac agcgcctgcg ccagcagttc caccacttcg cgctcgcgtg ccgacagcag
     5641 gaccggcgcc ggcgccatgc ctgctacgtt ttccgtagca cgcggcccgg ccacggcgtc
     5701 catggtggac tgcaggtact gcgcgatctc cgccgccggc gcggccatct gcagcagctc
     5761 gaccagcggc acggccgcgc tgccgaactc ggccagcacg cgcatctgcc cgagcgcggc
     5821 ggcctgcttc agcgcttcac gcgcggcggc cagcgcctgc ggcttgcggc cggcgcgcag
     5881 cagcgcgagc gcgcgcagca ggcgcaggcg cgtgtgctgc aggccgcgcc gctgggttag
     5941 tgcggcctgc tcggccgcat cgatctgcac caacacgtca tcctgttcct gcgcgaaggc
     6001 cgcctccgcg cgcgccatca gcgcggccag cacgatgccg ctgcgcgcac ccggcggctc
     6061 gttgcggtag cgcgcggcca gtgccgccag gcgccgcagc aggtccggcg tcgccgccgc
     6121 gttgccttgc gccagcgcca ggcgcagctg ctcgtacagg ctccacgcca ccaaccggtc
     6181 gagccgctgg cggtagccgg cttcctccag ccgctgcagc gtgtccgctg caccgcgcac
     6241 atcgccgtcg atctgctgca gccgcgcgcg cacgcgccag gcgtagagca ggccgtcggc
     6301 attgccgacc gcttcgatca ggtcaccgtg gcgctcgagg aaatcgcgcg cctgcgccag
     6361 ctcgcccgac tggtagtacg cctccagcag gtagctgagg aacaggccgg cggcatcggc
     6421 gccgaaggcg gtggtgcgca gcgcatcgct gatgtagggc gtgagcgagg cgatggccag
     6481 ctggatgtgg ccttccacca ggtgcgagaa cccggccacc acgtcgacca gcggccgcgg
     6541 gtactccggc cgcgatgcga tgtcatggcg atggcgcagg cgcgccacct cgcgcgcgcg
     6601 ctcgaactcg ccggcgtaaa ccagcgacag gctggcgatg ttgtagagga tcagcgcctg
     6661 gaacgggctg gccggtggcg ccggcaacat cggctcgacc atggccagcg aggcggcggt
     6721 gtcgtcggca cgcatggcgc ggtaggcctt gagcagcgcg agctcggtgg ccacttcggc
     6781 gcggcgcgcg ccggcatgcg cttccatggc ggcgatgctg cggtcgaact cgtcgatgcg
     6841 gctgcacgac agctgcgccc agccggcgca caggctggca ttgagccgcg actgcaggcg
     6901 ctcgcgcggc accgcgtcgc accacttgag gaactgcacg aagttggcgc tgttgatcat
     6961 gcgccgcgcg ccgcggtcga tcagctcgac catgaagtcg gcgtcgtcgg ccgcctgcgc
     7021 gtggcgcatg gcctccacgt gcaggctctt gccggcgaac cagtgcgcgg ccagctggtg
     7081 cagcttgcgc cgctcggctt cgtcgtggcg cgccagccgc gcgttgagga acgaggcgaa
     7141 cagccggtgg aagcgatacc acggctgtgg atcggcggtc tcgatcggga tcaggaaaag
     7201 gttttccgtc tcgaattttt ccagcagctc gccggcgcgc gggttgccgg tgagcagctc
     7261 gcacagctcg cggttgagcc ggcggcaggc ggagatgcgc gtgaggaaat ccagctcctc
     7321 ctccgacagg tgctcgatcg ccgccttctc caggtagccg ctgagcgctt cctcctgcga
     7381 cagcgacagc tgcccctggc gcgcgaacca ggcttccggc gcgggctcct tgcgcagcga
     7441 gaacgccagc agctgcaggc cggcagccca gccgtcggtg atctcgtgca gcgtgcgcac
     7501 ctgcgtcggc gtcagctgcg ccagcccctg cgcggcggtg aagcggcgcg tctcgtcgag
     7561 gtcgaagcgc agttcggcga agcgcagctc ggtcagctgg tcggccgcct gcagctgcgc
     7621 cagcgccagc ggcgggcgca cgcgcgtgcc cagcaccagg tggaagttgg ccggcgccag
     7681 cgccagccag cggtcgatca gctgcagcac gccgaacgag gcgatgtaat ggaaatcgtc
     7741 gatgaccagg tacacctcgc gcccgtgctg ctgcacgtcg ttgaccagcg cggcgagcag
     7801 cggcttgccg gccttgccgc cggagcgcgt gtacacgtgc agcgcctccg cgctgatggc
     7861 gacgccggcc tgcgccagcg cttccaccag gtacgcggcg atctgcgcat cgtcgtcgtc
     7921 ggcgccggcg ttgtaccagg cgatgctggc gccgtgcagg aacatctgct tgcgccactg
     7981 gatcagcagc gtggtcttgc cgcagccggc cggcccgaca atcagcgcca gcttgcgctc
     8041 gcgccgcgcc atcagcgcgg ccacctgcgc ctcgcgcg
//
LOCUS       DASZZC010000605         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1203218,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000605 DASZZC010000000
VERSION     DASZZC010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203218"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VHE37_12945"
     CDS             complement(<1..251)
                     /locus_tag="VHE37_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36487.1"
                     /translation="MKRLLVLLAALGLAGPALADHAAECAVARPLLDRSFPLPHVAQA
                     LGAHKLTVLVLGAGSSILPGATGAKKAYPARLQNALDEGL"
     gene            complement(252..1403)
                     /locus_tag="VHE37_12950"
     CDS             complement(252..1403)
                     /locus_tag="VHE37_12950"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HVT36488.1"
                     /translation="MAPVSTQRDLRLDLFRGLALWPIFLDHIPSNAVSWITIRNYGFS
                     DATEIFVFISGYTAAFVYGRAMEEHGFIVAGARVLKRAWQIYVAHVFLFAIYLAEIAY
                     VAQSFDNPLYTEEMGVLNFLNHPDVTIVQALLLKFKPVNMDVLPLYIVLLLLFAPILW
                     VLLRRPATALGLSVALYALTVHFEWNIPAWPKGVWVFNPFAWQLLFVFGAWCALGGAQ
                     KLKRVLDSRITFALAVAYLVFAFGIVMTWYFPWLASYVPKFVNDAIYPISKTNLDILR
                     LLHFLSLAVVTLWFVPRGWSVLNSRVFWPAIVCGQHSLETFCLGVFLAFAGHFVFTEV
                     SNRLLMHVIVSFTGIVIMVAVAALVSWYKRMERRGPRPPAALPAAAGGS"
     gene            1486..>1873
                     /gene="apaG"
                     /locus_tag="VHE37_12955"
     CDS             1486..>1873
                     /gene="apaG"
                     /locus_tag="VHE37_12955"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HVT36489.1"
                     /translation="MYRAVTRKIEVTVTPRFVQERSSPTNGYFFWAYTIDITNLGGET
                     VQLKTRHWRITDALGRLQEVKGPGVVGEEPVLKPGESFEYTSGVPLPTPSGFMTGSYG
                     MVTGEGEHFDIEIPAFSLDSSYAERTI"
BASE COUNT          415 a          572 c          604 g          282 t
ORIGIN      
        1 aggccttcgt ccagcgcgtt ctgcaggcgg gcgggatagg ccttcttggc gccagtcgcg
       61 ccaggcagga tcgaagagcc tgcgccgagc accagcacgg tcaacttgtg cgcgccgagt
      121 gcctgcgcga catgcggcaa cggaaaactg cggtcgagca gtggccgggc gaccgcgcat
      181 tccgccgcat ggtcggcaag ggctggcccg gccagcccca atgccgccag cagcaccaat
      241 agccgcttca tctatgagcc cccggcggct gccggcaacg cggccggcgg ccgcggcccg
      301 cgccgctcca tgcgcttata ccacgacacc agcgccgcca ccgccaccat aatgacaatg
      361 ccggtgaagc tgacgataac gtgcatcaat aggcggttgg acacctcggt gaacacgaag
      421 tgcccggcaa aggcaaggaa gacgccgagg cagaaggttt ccagcgaatg ctggccgcac
      481 acgatcgccg gccagaacac gcgcgaattc agcaccgacc aaccgcgcgg tacgaaccag
      541 agcgtcacca ccgccagcga caggaaatgc agcaggcgca ggatatcgag attggtcttg
      601 ctgatcggat agatggcgtc gttgacgaac ttcggcacgt agctcgcgag ccacgggaaa
      661 taccaggtca tcacgatgcc gaaggcgaag acgagatagg cgacggccag cgcgaaggtg
      721 atgcgcgagt cgagcacgcg cttgagcttc tgcgcgccgc cgagcgcgca ccaggcgccg
      781 aaaacgaaca gcaattgcca agcgaacgga ttgaacaccc aaacgccctt cggccaggcg
      841 ggaatgttcc attcgaaatg aacggtcaac gcgtaaagcg ccaccgacag cccgagcgcc
      901 gtcgccggcc ggcgcagcag cacccacagg atcggcgcga acagcagcag cagcacgatg
      961 tatagcggca gcacgtccat gttgaccggc ttgaacttca acagcagcgc ctggacgatg
     1021 gtgacgtccg gatggttgag gaaattgagc acgcccattt cctcggtata gagcgggttg
     1081 tcgaacgact gcgccacata ggcgatctcg gcgagataga tcgcgaacag aaacacgtgc
     1141 gcgacgtaga tctgccaggc gcgcttgagc acgcgggcgc cggcgacgat gaagccgtgc
     1201 tcctccatgg cgcgcccgta cacgaaggcg gcggtgtagc cggagatgaa gacgaagatc
     1261 tcggttgcat ccgagaagcc gtagttgcgg atggtgatcc agctcaccgc gttggacggg
     1321 atgtggtcga ggaagatcgg ccagagggcg agcccccgga acaaatccag ccgcaaatcg
     1381 cgttgggtcg aaacgggggc catgaacggc ttttctttcg tgcgctgttt cgcgataatt
     1441 aaattgagca acgacgatgg attcgggtca gattagggct tttcgatgta ccgcgcggta
     1501 acccgcaaaa ttgaggtgac ggtcacgccc cgcttcgtgc aggagcgctc gtcgcccacg
     1561 aacggctatt tcttctgggc ctacaccatc gacatcacta acctcggcgg cgagacggtg
     1621 cagttgaaga cccggcactg gcgcatcacc gacgcgctcg gccgcctgca ggaggtcaag
     1681 ggtcccgggg tggtgggaga agagccggtg ctcaagcccg gcgaatcctt cgaatatacc
     1741 agcggggtac cactgccgac gccatccggt ttcatgaccg gcagctacgg gatggtgacg
     1801 ggggaaggcg agcatttcga tatcgagatc ccggcattct cactggattc gtcctacgcc
     1861 gaacggacga tca
//
LOCUS       DASZZC010000606         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_304162,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000606 DASZZC010000000
VERSION     DASZZC010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304162"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..902
                     /locus_tag="VHE37_12960"
     CDS             48..902
                     /locus_tag="VHE37_12960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVT36490.1"
                     /translation="MKYFAWILMLLACVPLHAQEHVHDGGDYSTRMAEQHRNDAPVAS
                     PAAQAKAQVAVSGSMVQYGDVDGAPVAGYLAFPVNHYQPLPGVLMFHEWWGLNDNIRS
                     MARQLAAHGYVVLAADLYRGQVADRPDAAKPLMQDALAHPQQMQQNISAGYEYLRQHL
                     HATRVGTLGWCFGGGVSLQAALLLGDRSSATVIYYGQLTDDADQLRKLHSPVLGLFGA
                     LDQGITVDSVKKFEAALQAQGRQPDIHIYPGAGHAFANPSGMAYQADAAQDAWRRTLA
                     FLRQQLQR"
     gene            complement(962..1171)
                     /locus_tag="VHE37_12965"
     CDS             complement(962..1171)
                     /locus_tag="VHE37_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVT36491.1"
                     /translation="MSNRESGTVKWFDNAKGFGFIQRAAGEDVFVHFRQIRGEGYRTL
                     QEGQQVQFNVVQGPKGLQAEDVSAV"
     gene            complement(1310..2500)
                     /locus_tag="VHE37_12970"
     CDS             complement(1310..2500)
                     /locus_tag="VHE37_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HVT36492.1"
                     /translation="MIRLYLFALAAAVVGLAAASWLRADVGYVLVSYRQWIVETSILG
                     LVAAGVAGLLLLYLAARLLLLAVRLPTTLREVAQRRRGDRAQESFEAGLLKLLEGNWR
                     RAEIELVRRAADHHAAHLNYLGAARAAQRVGAGDRRDHYLRLAARSAPELEFATLLTQ
                     AELQRERGEYALARDTALALRNREPMHPYAIELLAECYAALRAWEALRALLPEAEKAS
                     ALTPSRVRELMIRAMTEMMRAAVDEARLDRLKSIWDCAPARFRDELEVRRAYARGLAR
                     LNADAEALALITGTLAKNWDGEMVLLYGELHAVDPLAQLATIEQWLNQYGEKHELLVT
                     AGRACLRNKLWGKARSYLEAVLRVAPSPAAYLELAHLCEQTQNTVEANRWYQQGLEMA
                     AQSA"
     gene            complement(2497..3531)
                     /locus_tag="VHE37_12975"
     CDS             complement(2497..3531)
                     /locus_tag="VHE37_12975"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HVT36493.1"
                     /translation="MSEISAATPVAPAPPRRARSGWGRALLLLVLLLLAAGSVTGYLW
                     LRDAQQSVEALEQKQKQLLVRVASLETANAEISQSVSRDTASLAEFGERLDHYDLIAG
                     KLAEELQGGRVRFTLQSVENLLLAANERVQLQHDIRGAAAALDLADQRLAGLSEPRLF
                     KLREEIARERSALLALPEPDLTSAALTLSSLIARAPDLPLRTRIPSRSLTLEETVEPP
                     PAQAWSDSLWSNVKLALRSVFAVRRADGPVPRLLPADQEAVVYQVLALKLEGVRMAML
                     RGDATSYRDLCDSAAAWLRQYFRPDDPGVMAATAELERLRALTLEAPAADITRSLTLL
                     RTYLNAPAQQ"
     gene            complement(3528..4289)
                     /locus_tag="VHE37_12980"
     CDS             complement(3528..4289)
                     /locus_tag="VHE37_12980"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HVT36494.1"
                     /translation="MSAAAALAGLRVLVTRPERQAEKLARLIEQAGGKAVRLPLLSIV
                     PAPPTPHIQRVLNDAHVFDWWIFTSANAVRYARELDRGAWPVRLAAIGPATAASLEAE
                     GLPALAPVASSSSEALLALPEFAEVDEQSILIVTGAGGLDQLAPALRARGARVEIAEV
                     YRRVPLPYDEARVVAALRGTDVIVITSGEALQHLYGLTPEASRAGLLKKHLVAPSARV
                     VEHASQLGFSMAVAVEQMSDAGLLQALETIAKKTT"
     gene            complement(4286..>4529)
                     /locus_tag="VHE37_12985"
     CDS             complement(4286..>4529)
                     /locus_tag="VHE37_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HVT36495.1"
                     /translation="ERALNAQLGGACQVPVAGHARIAGGELVLQALVGAPDGSRLVRD
                     EIAGPVTDAAALGVQLAQRLLAAGARDILATIGVHV"
BASE COUNT          676 a         1632 c         1548 g          673 t
ORIGIN      
        1 cgacgcgctg aagacatagt tttcggttcg ttcacggagg aacgcacatg aaatatttcg
       61 cctggatact catgctgctt gcgtgcgtgc cactgcatgc gcaggagcac gtgcacgacg
      121 gcggcgacta cagcacgcgc atggccgagc agcacaggaa cgacgcgccg gtggccagcc
      181 cggccgcgca ggccaaagcc caggtcgcag tgagcggctc aatggtgcaa tacggcgatg
      241 tcgacggcgc gccggtggcg ggctacctcg ccttcccggt caaccactac cagccgctgc
      301 ccggcgtgct gatgttccac gaatggtggg ggctcaacga caacatccgc tcgatggcgc
      361 ggcagctggc ggcgcatggc tacgtggtgc tggccgccga cctctaccgt ggacaagtgg
      421 ccgacaggcc cgacgcggca aagccgctga tgcaggacgc gctggcgcat ccgcagcaga
      481 tgcagcagaa catctcggcc ggatacgagt acctcaggca gcatctccac gccacgcgcg
      541 tgggcacgct gggctggtgc ttcggcggcg gcgtatcgct gcaggctgcg ctgctgctcg
      601 gcgacaggtc cagcgccacg gtcatctact acggccagct caccgacgat gcagaccagc
      661 tgcgcaagct gcactcaccg gtgctcggcc tgttcggcgc gctggaccag ggcatcaccg
      721 tggacagcgt gaagaaattc gaagccgcgc tgcaggcgca aggcaggcag ccggacatcc
      781 acatctatcc cggcgcgggc cacgccttcg ccaatccttc cggcatggcc taccaggccg
      841 acgcggcaca ggatgcgtgg cgccgcacgc tggccttcct gcggcagcag ctgcagcgct
      901 aggcaaaaaa aagccgcgcg ggactttcgt ccccgcgcgg catgttgtct cgccggctgc
      961 gtcagaccgc agacacgtcc tccgcctgca ggcccttggg gccctgcacc acgttgaact
     1021 gcacctgctg gccctcctgc agcgtgcggt atccctcacc gcggatctgc cggaaatgga
     1081 cgaacacatc ctcgcccgcg gcgcgctgga tgaagccgaa cccctttgcg ttgtcgaacc
     1141 atttcacggt tccgctctcg cgattggaca tagatcaacc ttcccttgaa tcccctctgt
     1201 gttgatttgc gcgcctcggc catggctggc gcgggcgcat cccttgggtc tccccgcgcc
     1261 cgactttacc gagttttacc tgccgccgcg acgggcggaa acgcggtctt caggccgatt
     1321 gcgcggccat ttccagaccc tgctggtacc agcggttggc ctcgaccgtg ttctgcgtct
     1381 gctcgcacag gtgcgccagt tccaggtacg cggcgggcga cggcgccacg cgcagcaccg
     1441 cttccaggta actgcgcgcc ttgccccaca gcttgttgcg caggcaggcg cggccggcgg
     1501 tgaccagcag ttcgtgcttc tcgccgtatt gattgagcca ctgctcgatg gtggccagct
     1561 gcgccagcgg atcgaccgcg tgcagctcgc catagagcag caccatttcg ccgtcccagt
     1621 ttttcgccag cgtgccggtg atcagcgcca gcgcctcggc atctgcgttg agccgcgcca
     1681 ggccgcgcgc ataggcgcgg cgcacttcga gctcgtcgcg gaagcgcgcc ggcgcgcagt
     1741 cccagatcga cttgagccgg tccagccggg cctcgtcgac cgccgcgcgc atcatctcgg
     1801 tcatcgcgcg gatcatcagt tcgcgcacgc gcgaaggcgt cagcgcgctg gccttctccg
     1861 cttccggaag caaggcgcgc aaggcttccc acgcgcgcaa ggcggcatag cactcggcca
     1921 gcagctcgat ggcatacgga tgcatcggct cgcggttgcg cagggccagc gcggtgtcgc
     1981 gcgccagcgc atattcgccg cgctcgcgct gcagttccgc ctgcgtcagc agcgtggcga
     2041 actccagctc cggcgcgctc ctggccgcca gccgcaggta atgatcgcgc cggtcgccgg
     2101 cgcccacgcg ctgtgccgcg cgcgcagcgc cgagatagtt gagatgcgcc gcgtggtgat
     2161 cggccgcgcg ccgcaccagc tcgatctcgg cccggcgcca gttgccttcc agcagcttga
     2221 gcagcccggc ctcaaacgat tcctgcgcgc ggtcgccgcg gcggcgctgc gccacttcgc
     2281 gcagcgtggt gggcaggcgc accgcgagca gcagcagccg ggccgcgagg tacagcagca
     2341 gcaacccggc cacaccggcg gcgaccaggc cgaggatcga ggtctcgacg atccactggc
     2401 ggtagctcac cagcacgtag ccgacgtcgg cacgcagcca cgaggccgcc gccaggccga
     2461 ccactgccgc cgccagcgca aacaggtaga ggcggatcat tgctgcgcgg gcgcgttgag
     2521 gtacgtgcgc agcagcgtca ggctgcgcgt gatgtcggcg gcaggcgctt ccagcgtgag
     2581 cgcgcgcagg cgctccagtt cggcggtcgc cgccatcacg cccggatcgt cggggcggaa
     2641 atactggcgt agccaggcgg cggccgaatc gcacaggtcg cggtagctgg tggcgtcgcc
     2701 gcgcagcatg gccatgcgca cgccttcgag cttgagcgcc agcacctggt agaccaccgc
     2761 ttcctggtcc gccggcagca gccgcggcac cggtccgtcg gcgcggcgca ccgcgaacac
     2821 gctgcgcagc gccagcttga cgttgctcca caggctgtcg ctccaggcct gcgccggcgg
     2881 cggctcgacc gtttcttcca gcgtcagcga gcgcgacggg atgcgcgtgc gcagcggcag
     2941 gtcgggcgcg cgcgcgatca ggctggacag cgtcagcgcg gccgaggtga ggtcgggctc
     3001 cggcaaggcc agcagcgcgc tgcgttcgcg cgcgatctcc tcgcgcagtt tgaacaggcg
     3061 cggctccgac aatccggcca ggcgctggtc ggccaggtcc agcgccgccg cggcgccgcg
     3121 gatgtcgtgc tgcagctgca cgcgctcgtt ggccgccagc agcaggttct ccaccgactg
     3181 cagggtgaag cgcacgcgcc cgccctgcag ttcctcggcc agcttgccgg cgatgaggtc
     3241 atagtggtcc aggcgctcgc cgaattctgc gagcgacgcc gtatcgcggc tgacgctctg
     3301 cgagatttcc gcgttggcgg tttccagcga ggccacgcgc accagcagct gcttctgctt
     3361 ctgttccagt gcttccaccg actgctgcgc gtcgcgcagc cacaggtagc cggtgacgct
     3421 gccggccgcg agcagcagca ggaccagcaa cagcagtgcg cggccccagc cgctgcgtgc
     3481 gcgccgcgga ggcgccggcg cgacgggcgt cgcggcggaa atttcgctca tgtggtcttc
     3541 ttggctatgg tttccagcgc ctgcagcagg ccggcgtcgg acatctgctc gacggccacg
     3601 gccatgctga agcccagctg ggaggcgtgt tctaccacac gcgcgctcgg cgcgaccaga
     3661 tgctttttca gcaagccggc gcgcgaagct tccggcgtca ggccgtacag atgctgcagg
     3721 gcctcgccgc tggtgatgac gatgacatcg gtgccgcgca gcgccgccac cacgcgcgcc
     3781 tcgtcgtagg gcaggggcac gcggcgatag acctcggcaa tttccacgcg ggcgccgcgc
     3841 gcgcgcagcg ccggcgccag ctggtcgaga ccgccggcgc cggtgacgat gaggatgctt
     3901 tgctcgtcca cctcggcgaa ctccggcagc gccagcagcg cctcgctcga cgatgacgcc
     3961 accggcgcca gcgccggcag gccctcggcc tcgagcgacg ccgcagtggc cgggccgatc
     4021 gccgccagcc gcaccggcca ggcgccgcga tcgagctcgc gcgcgtagcg caccgcgttg
     4081 gcgctggtga agatccacca gtcgaacaca tgcgcgtcgt tgagcacgcg ctggatatgc
     4141 ggcgtgggcg gcgccggcac gatggacagc agcggcagcc gcaccgcctt gccgccggcc
     4201 tgctcgatca ggcgcgccag cttttccgcc tgccgttcgg gccgcgtgac cagcacgcgc
     4261 aaacccgcga gcgcggcggc ggcgctcaca cgtgcacgcc gatggtggcg aggatgtcgc
     4321 gcgcgccggc cgccagcagg cgctgcgcca gttgcacgcc cagcgctgcc gcgtccgtta
     4381 ccgggccggc gatttcgtcg cggaccaatc gggaaccatc cggcgcgccg accagcgcct
     4441 gcagcaccag ctcgccgccg gcgatgcgcg cgtggcctgc tacgggcacc tggcaggcgc
     4501 cgccgagctg tgcgttgagc gcgcgctcg
//
LOCUS       DASZZC010000607         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_623463,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000607 DASZZC010000000
VERSION     DASZZC010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623463"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..858
                     /gene="ubiG"
                     /locus_tag="VHE37_12990"
     CDS             157..858
                     /gene="ubiG"
                     /locus_tag="VHE37_12990"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HVT36496.1"
                     /translation="MTPNVDTEEIARFEKLAATWWDPAGEMGPLHAINPPRLRYIEQC
                     AGGIQGKRALDVGCGGGILAEALARAGAQTLGIDLAEASLDVARKHAADAGVQVEYRG
                     IAAEALATEMPAAFDLVCCLEMLEHVPDPQSVIAACAALVKPGGTVVFSTINRNPKAY
                     ALAVVGAEYLLNLIPRGTHDYAKFIRPSELERWATAAGLQLKGLKGLRYNPLLKSASL
                     ADDVDVNYLMHCVRA"
     gene            855..1508
                     /gene="gph"
                     /locus_tag="VHE37_12995"
     CDS             855..1508
                     /gene="gph"
                     /locus_tag="VHE37_12995"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650418.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HVT36497.1"
                     /translation="MTRARCVLFDLDGTLVDTAPDLGHAANCVRDELGMAPLPIAQYR
                     PVASAGARGLLGVALGITPAHEDFPRHRDRFLDLYRGHLSRASKLFDGLDAQLRQFEE
                     AGVRWGVVTNKPGWLTRPLLEDLQLAARAACAVSGDDVPKAKPAPDSLLLACRQLALT
                     PADCIYVGDDARDIAAARAAGMRSVAVRWGYFGEGGPVENWGADRIIDHPRGLADCL"
     gene            1636..2382
                     /locus_tag="VHE37_13000"
     CDS             1636..2382
                     /locus_tag="VHE37_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36498.1"
                     /translation="MSNPIPVNYQPAPALLAGRAILITGAGDGLGRAAAVACARAGAT
                     VVLLGRTVAKLEKTYDLVEADNAPKPAIYPLNLAGASFKDYQELAITLEKEIGRLDGL
                     VHCAAHFKQYAPLADLDPREWVESLQVNLTAAQALTQACMPLLARAPDASVVFVSDES
                     GRKAKPFQGAYGISKAAIEQLMIMWALEMKNRPTVRFNSYDPRPMRTALRARGYASEV
                     VEKLPPPEAAVPGLLWLLGPDSAGVSGRAC"
     gene            complement(2395..>3192)
                     /locus_tag="VHE37_13005"
     CDS             complement(2395..>3192)
                     /locus_tag="VHE37_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36499.1"
                     /translation="GDTLATVTFTDGSTDDVKAGQGLSIAAGVHMRPERNAHFDLRAL
                     VGYKYETTKADNADIHISRVTWELLPTYRFDGRWWASVGLAKHTGVKLTLGGFGPDVK
                     LHSNVGPELQIGWSYFALTYTSLRYSDDFGDSAKANSFGIRFTSAFGDNCCGWHQKAN
                     SSPTPPTSPYAPPPASVPVPLAPAAVSAPPALQAGMAAASLPGAQLRKRPLVNDAGTA
                     LAAGSAVTLEQAYPSEAGSWWYVGLADGSHGWLREGDVGPAPAPATP"
BASE COUNT          530 a         1107 c         1056 g          499 t
ORIGIN      
        1 atcgcgcggc catcactgcg cgtgcggccg ggtggcgcgc gaagatcaaa ccctgaaaac
       61 cttcccgtca gtcccgcgca agcgggaatc catcgatcaa ccacgcatgc atttcaacca
      121 tgggtttccg cttgcgcggg aatgacggca aaaaaaatga cccccaacgt cgacaccgaa
      181 gaaatcgcgc gcttcgaaaa gctcgccgcc acctggtggg acccggccgg cgagatgggc
      241 ccgctgcacg cgatcaatcc gccgcggctg cgctacatcg agcaatgcgc cggcggcatc
      301 cagggcaagc gcgcgctgga cgtcggctgc ggcggcggca tcctcgccga agcgctcgcg
      361 cgcgccggtg cgcagacact cggcatcgac ctcgccgaag cctcgctcga cgttgcgcgc
      421 aagcacgccg ccgacgccgg cgtgcaggtc gagtatcgcg gcatcgccgc cgaagcgctc
      481 gccacggaaa tgccggcagc cttcgacctg gtctgctgcc tggaaatgct cgagcacgtg
      541 ccggatccgc agagcgtgat cgccgcctgc gccgcgctgg taaagccggg aggcacggtg
      601 gtgttctcca ccatcaaccg caatcccaag gcctacgcgc tcgcggtggt cggcgccgaa
      661 tacctgctga acctgattcc gcgcggcacg cacgactacg ccaagttcat ccgcccatcc
      721 gagctggaac gctgggcgac ggcggcgggc ctgcagctga aaggcctgaa gggcctgcgc
      781 tacaacccgc tgctgaagag cgcatcgctg gccgacgatg tcgacgtgaa ttacctgatg
      841 cattgcgtgc gcgcatgacg cgagcgcgct gcgtgctgtt cgatctcgac ggcaccctgg
      901 tcgacactgc gcctgatctc ggccacgccg ccaactgcgt gcgcgacgag ctcggcatgg
      961 cgccgctgcc gatcgcgcaa tatcgcccgg tggcctcggc cggcgcgcgc ggactgctcg
     1021 gcgtggcgct cggcatcaca ccggcgcatg aagatttccc gcgccaccgc gatcgcttcc
     1081 tcgatctcta tcgcggtcac ctctcgcgcg cgtcgaaact gttcgacggg ctcgacgcgc
     1141 aattgcgcca attcgaggag gccggcgtcc gctggggcgt ggtgaccaac aagccgggct
     1201 ggctgacgcg gccgctgctg gaagacctgc agctggccgc gcgtgccgcc tgcgccgtga
     1261 gcggcgacga cgtgcccaag gccaagcccg cgccggattc gctgctgctc gcgtgccggc
     1321 aactcgcact cacgccggcc gactgcattt acgtgggcga cgacgcgcgc gacatcgccg
     1381 ccgcgcgcgc cgccggcatg cgcagcgtcg ccgtgcgctg gggttatttc ggcgaaggcg
     1441 gcccggtgga gaactggggc gcggaccgca tcatcgacca tccgcgcggg ctcgcggatt
     1501 gcctctgacg cttccaccgc accttgtttt ttgatgttaa tatttattgt atattttaat
     1561 aataatatta ttttttataa caagcaaaat ggcaatgcgg ttgagtagaa aatccgcgct
     1621 gcacgagacc aatccatgtc caacccgatc cccgtgaatt accaacccgc gcccgccctg
     1681 ctcgccggcc gcgccattct catcaccggc gcaggcgacg gcctgggccg agctgccgcc
     1741 gtcgcctgcg cgcgcgccgg cgccaccgtg gtcctgctcg gccgcaccgt ggccaagctg
     1801 gaaaaaacct acgacctggt cgaagcggac aacgcgccga agccggcgat ctacccgctc
     1861 aacctggccg gcgcctcgtt caaggactac caggaactgg ccatcacgct ggaaaaggaa
     1921 atcggccggc tcgacgggct ggtgcattgc gccgcgcatt tcaaacagta cgcgccgctg
     1981 gccgatctcg atccgcgcga gtgggtggaa agcctgcagg tgaacctcac cgccgcgcag
     2041 gcgctgacgc aggcgtgcat gccgctgctg gcgcgcgcgc cggatgcgtc ggtggtgttc
     2101 gtcagcgacg aatcggggcg caaggccaag ccgttccagg gcgcgtacgg catcagcaag
     2161 gccgccatcg aacagctgat gatcatgtgg gcgctggaga tgaagaaccg gccgacggtg
     2221 cgcttcaact cgtacgaccc gcgccccatg cgcaccgcgc tgcgcgcgcg cggctacgcc
     2281 agtgaagtgg tggagaaact gccgccgccg gaagccgctg taccaggcct gctctggctg
     2341 ctcggccccg acagcgcggg cgtgagcggc cgcgcctgct gaggcgcgtg caggctacgg
     2401 cgtcgcgggc gcgggcgccg ggcccacgtc gccttcgcgc agccagccat ggctgccgtc
     2461 agccaggcca acgtaccacc agctgccggc ttcgctcgga tacgcctgct ccagcgtcac
     2521 tgcgctgccg gcggcgagcg cagtgcccgc atcgttcacc agcggacgct tgcgcagttg
     2581 cgctcccggc aggcttgccg cggccatgcc ggcctgcaac gccggcggcg ccgacaccgc
     2641 tgcgggcgcc aagggtaccg gcacgctcgc tggcggcggc gcatacggtg atgtgggcgg
     2701 cgtgggcgac gagttcgcct tctgatgcca gccgcagcag ttgtcgccga atgcgctggt
     2761 gaagcggatg ccgaaggagt tggccttggc cgaatcgccg aaatcgtcgg aatacctgag
     2821 cgaggtgtag gtgagcgcga agtagctcca gccgatctgc aactcgggcc cgacgttgga
     2881 atgcagtttc acgtccggcc cgaaaccgcc cagcgtcagc ttgacgccgg tgtgcttggc
     2941 cagcccgacg ctggcccacc accggccgtc gaaacggtag gtgggcagca gttcccaggt
     3001 gacgcgcgag atgtggatgt cggcgttgtc ggccttggtc gtctcgtact tgtagcccac
     3061 cagcgcacgc aggtcgaaat gcgcattgcg ctccggccgc atgtgcacgc ccgccgcgat
     3121 ggacaagccc tgcccggcct tgacgtcgtc ggtgctgccg tcggtgaagg tcaccgtggc
     3181 cagcgtgtcg cc
//
LOCUS       DASZZC010000608         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_242951,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000608 DASZZC010000000
VERSION     DASZZC010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242951"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VHE37_13010"
     CDS             complement(<1..1700)
                     /locus_tag="VHE37_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT36500.1"
                     /translation="MNVVDLSTRRPVAITMLTLAVLLFGMVSMSRLQVTLLPDLSYPT
                     LTVRTGLPGAAPTEVETLLSKPIEEALGTIKGVREVSSVARAEQSDVTLEFNWGTKMD
                     YAVLDVREKLDALQLPKEAQHPVVLRFDPSQDPIMRTGLALKDKPGRVIDDAALKRLR
                     RVAEDLIKKPLEGVDGVAAVKISGGLEDEVQVLVDLEKLSQLKLTLSEVAARLSAENA
                     NISGGRVDQGAASYLVRTLNQFKTLDEMADTIITTRDGRNIYVRDIAEVRAGHKEREA
                     VIRIAGEEAVEISIYKEGDGNTVHIAEGVKKRLEELGKSLPDDLELKPLYDQSVFIKQ
                     ATEDVTHSAIEGGLLAILIVYLFLRNAWTTAVISLAIPVSIIATFNLMYGARLSLNIM
                     SLGGMALAIGLVVDDAIVVLENIYRKHEQGLSLKQAAVAGSSEVAGAVTASTLTTVAV
                     FFPMVFVQGIAGQLFSDQALVVSGALVFSLIVSFTLTPMLASLDFGLTPLAPAAPLVS
                     DGAKWRRGLSRARVAALETAPMVLLRVLVWIARAVALAIGSVMRPLAGGWQRGYAALE
                     S"
     gene            complement(1726..>2385)
                     /locus_tag="VHE37_13015"
     CDS             complement(1726..>2385)
                     /locus_tag="VHE37_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT36501.1"
                     /translation="GARAAYDLAQLQLKDAEIRAPFAGVVTARYIKRGNTIQAGTQAF
                     RVTQMDRLQANLNVPERDIHKLKPGHPVKLTLDAWPGQVFNGQIVRINPVVDASSGTV
                     KVTAQMQPQDELKPGMFGRVEILYDHRDNAILVDRDAVMTEDQQQSVFVVDAGKARRK
                     LIKTGYSDADHFEVVNGLKPGERVVTTGQSSLKDDARVEAITAGGANSLGGGATQAAA
                     R"
BASE COUNT          364 a          803 c          807 g          411 t
ORIGIN      
        1 gactccagcg cggcgtagcc gcgctgccag ccgccggcca gcgggcgcat gacgctgccg
       61 atggccagtg cgaccgcgcg cgcgatccac accagcacgc gcagcaggac catcggcgcg
      121 gtctccagcg ccgcgacgcg cgcgcgcgac aggccgcgac gccacttcgc gccgtcactc
      181 accagcgggg cggcgggcgc cagcggcgtg aggccgaaat ccagcgaggc cagcatcggc
      241 gtcagcgtga acgacacgat cagcgagaac accagcgcgc ccgacaccac cagcgcctgg
      301 tcggagaaca gctggccggc gatgccctgc acgaacacca tcgggaaaaa caccgcgacg
      361 gtagtcagcg tcgacgcggt caccgcgccg gccacttccg acgagccggc cacagcggcc
      421 tgcttcagcg acaggccctg ttcgtgtttc cggtagatgt tctccagcac cacgatggcg
      481 tcgtccacca ccaggccgat ggccagcgcc atgccgccga ggctcatgat gttgagcgac
      541 agccgcgcgc cgtacatgag attgaaggtg gcgatgatcg acaccgggat ggccagcgag
      601 atcaccgcgg tggtccaggc gttgcgcagg aacaggtaga cgatgaggat ggccagcagg
      661 ccgccctcga tggcgctgtg ggtcacgtcc tcggtggcct gcttgatgaa gacggactgg
      721 tcgtagagcg gcttgagttc gagatcgtcg ggcagcgact tgcccagttc ttccagccgt
      781 ttcttcacgc cttcggcgat gtgcacggtg ttgccgtcgc cttccttgta gatggaaatc
      841 tcgaccgctt cctcgccggc gatgcggatc accgcttcgc gctccttgtg gccggcgcgc
      901 acctcggcga tgtcgcgcac gtagatgttg cggccgtcgc gcgtcgtaat gatggtgtcg
      961 gccatctcgt ccagcgtctt gaactggttg agcgtacgca ccaggtaaga ggccgcgccc
     1021 tggtcgacgc ggccgccgga gatgttggcg ttttccgccg acaggcgcgc agcgacttcg
     1081 gacaatgtca gcttgagctg ggagagtttt tccaggtcga ccagcacctg cacttcgtct
     1141 tccaggccgc cggagatttt caccgcggcc acgccgtcga caccttccag cggtttcttg
     1201 atcaggtctt cggccacgcg gcgcaggcgc ttgagcgcgg catcgtcgat cacgcggccg
     1261 ggcttgtcct tcagcgcgag gccggtgcgc atgatcgggt cctgcgacgg atcgaaacgc
     1321 agcaccaccg ggtgctgcgc ctccttgggc agctgcagcg cgtcgagctt ctcgcgcacg
     1381 tcgagcaccg cgtaatccat cttggtgccc cagttgaatt ccagggtcac gtcgctctgc
     1441 tcggcgcgcg ccaccgaact cacctcgcgc acgcccttga tggtgcccag cgcctcctcg
     1501 atcggcttgg acagcagcgt ctccacctcg gtcggcgcgg cgccaggcag gccggtgcgc
     1561 acggtcagcg tgggatacga caggtcgggc agcagcgtca cctgcaggcg cgacatcgag
     1621 accatgccga acagcagcac ggccagcgtc agcatggtga tggcgaccgg gcgccgggtg
     1681 gacaggtcga cgacgttcat gcgtgcactc gcgtccgctg cagtgtcagc gcgccgctgc
     1741 ctgcgtggcg ccgccgccga gcgagttggc gccgccggca gtgatggcct ccacgcgcgc
     1801 gtcatccttc aggctgctct gccccgtggt gaccacgcgc tcgcccggct tgaggccatt
     1861 gaccacttcg aagtgatcgg catcgctgta gccggtcttg atcagcttgc gccgcgcctt
     1921 gccggcgtcg accacgaaaa ccgactgctg ctggtcttcg gtcatcaccg cgtcgcggtc
     1981 gaccaggatg gcgttgtcgc ggtggtcgta gaggatttcc acgcggccga acatgccggg
     2041 cttgagctcg tcctgcggct gcatttgcgc ggtgaccttc accgtgccgc tggaggcgtc
     2101 aaccaccggg ttgatgcgca cgatctggcc gttgaacacc tggccgggcc aggcgtcgag
     2161 cgtgagcttc accgggtggc cgggcttgag cttgtggatg tcgcgctcgg gcacgttgag
     2221 gttggcctgc aggcgatcca tctgcgtcac gcggaacgcc tgcgtaccgg cctggatggt
     2281 gttgccgcgc ttgatgtagc gcgcggtgac cacgccggcg aacggtgcgc gtatttcggc
     2341 atccttcaac tgcagctggg cgaggtcgta ggcggcgcgc gcgcc
//
LOCUS       DASZZC010000609         6289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1265198,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000609 DASZZC010000000
VERSION     DASZZC010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265198"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VHE37_13020"
     CDS             complement(<1..698)
                     /locus_tag="VHE37_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HVT36502.1"
                     /translation="MNAVLKSAGFTDFKVKDMSLAEFGRKRIGMAQEEMPGLMSIRAK
                     YAPLQPLKGVRLTGSLHMTKETAVLLETLKALGASVRWASCNIFSTQDDAAAAVAAAG
                     TPVFAWKGETLEEYWDLTLDAVTHKLPDGTLGGPELVVDDGGDVTLLIHKGYELEQGD
                     KWVDTPSDNHEVSVIKNLLKKVAKERPGFWTRVVKGWRGVSEETTTGVHRLYQMLEAG
                     KLLVPAINVNDSVT"
     regulatory      complement(711..791)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(810..2009)
                     /gene="metK"
                     /locus_tag="VHE37_13025"
     CDS             complement(810..2009)
                     /gene="metK"
                     /locus_tag="VHE37_13025"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HVT36503.1"
                     /translation="MSSYLFTSESVSEGHPDKIADQISDAILDSILAQDKKARVACET
                     LVKTGVVVVAGEITTTAWSDFEAIARKVVTDIGYDNSAVGYDGHTCGVLNFIGKQSPD
                     INMGVDRKSPEEQGAGDQGLMFGFACTETPDLMPAPIYYSHRLVERQTKVRKAKNSPC
                     AWLRPDAKSQVTLAYDGNKIVGIDAVVLSTQHSPDVSLKKLREGVMEEVILPVLPAKW
                     ITKKTKFHINPTGNFVIGGPVGDCGLTGRKIIVDSYGGYARHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLADKCEIQVSYAIGVAEPTSISITTFGTGKISDDRIEKLVRA
                     HFDLRPYGIIKMLDLIHPMYQVTASNGHFGRKPYEVKGADGKKFTAFSWEKTDKADEL
                     RAAAGLK"
     gene            2201..4690
                     /locus_tag="VHE37_13030"
     CDS             2201..4690
                     /locus_tag="VHE37_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT36504.1"
                     /translation="MALADSQPDSALPVVFELLERSHYHFQAACVANYTAALLMLLTG
                     ALMLRWERGNRTSRLFAGYTVLFALWSANRATMHLLDDPGLVVLLGRLHYCLVSIALP
                     LLLEFIFLLLRIERQRRSLVRYNQALGLLLAATALQTPRVISGAAHFPWGYDPQLGPA
                     GLAMMVWIFMLMCQTSLDLYRARRRTRPRTQERRRLNLFVVAVFFLFGAANDFLVDLG
                     LPVYPLGVLPVLCFTLLTGWLTWRYGLVEISAQLAADKIAELARGALLMLDADGVIQF
                     TNISAIKMLGQPRKRLLGSSASSLLGDAFAVDNLARLAALEGRQVEKQLFYLGPEEMV
                     RELAFSVSAVRDNHQRDVAYVCMVRDVTEQKQAEQRRAAEGLKDPLTGLPNRVLFLEL
                     LDNAVKRARESRGYGFCLFFLGIDRMRVINEDLGYATGDRLLVEVAARLQQLAGPQDA
                     LARVGGDEFCLLVETPSGAEQARELVARIEQAVRAPLSLADHKLYLSASIGVAASGLE
                     YASGADMLRTASIALYRAKESGGGGVQFSTANEGGSQRTRLESELRRAVELQEFCVYY
                     QPIIDLTEERVDGFEALVRWRHPERGLLAPSEFLELAEEVGLIAAIDNQVLRTACADL
                     KELQRLAGDFQLKVSVNLADQELLDPGFTDRVQALLSQHDLAAGTVSLELLERVAQVE
                     PVRETLRQLRLIGVGLYIDDFGTGWSSLSRLHDLPASVLKIDRDFVRAMSLGDGGEKI
                     ISSIVVLARNLGMGVVAEGASIADEVRHLHRLGCRYVQGYFFAHAMPFEGAAELLRDP
                     RMLQQKFSLLDLDDQRMRPVDYPKRSAENSG"
     gene            4706..5260
                     /locus_tag="VHE37_13035"
     CDS             4706..5260
                     /locus_tag="VHE37_13035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT36505.1"
                     /translation="MALTPSTMLPLGTLAPPFSLPDVVSGNTLELARLKSAQATLVMF
                     ICNHCPYVKHVRAQLVQLARDYQPRGAAIIAISSNDAQKYPEDAPARMRETALEHHYP
                     FVYLYDETQEVARAYRAACTPDFFLFDGALKLAYRGQLDDSRPGNGKPVTGRDMRSAL
                     DALLAGRAPSPEQQPSVGCNIKFR"
     gene            5272..6288
                     /locus_tag="VHE37_13040"
     CDS             5272..6288
                     /locus_tag="VHE37_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36506.1"
                     /translation="MKKSQGCWLLIALGAVTANAYADGCGKERALNAAEQKFVADHLA
                     AARAAFPAPPAGWARYEPDAGLRARVAQDLPPPTTVCEQWGPRPLSLSAHATYTPADS
                     ALMRDLQKQRDEQAQQKKVAELQQKMQEAGARGDYNAMASLNQQMQAASMPAGAKAAA
                     PMSDAEYDRQNEALSKKLEAAQARSDYTAMQQISAQQMALVMRKAGVDTSGVAAAEIT
                     SDQINAAPRVHVVVEFNRDAFDIPENAKKVSYGGAAVGYAARGDHFCYDNGGGSDREQ
                     VYLLFGPWQGKTNYNNQLANYISDAADHGAFASQVTTLVSICGDPAMVEKLAPAIRYD
                     ALRR"
BASE COUNT         1001 a         2062 c         2147 g         1079 t
ORIGIN      
        1 ttggtgaccg agtcattcac gttgatggcc ggcacgagca gtttgcccgc ttcgagcatc
       61 tggtagaggc ggtgcacgcc ggtggtggtc tcctcggaga cgccgcgcca gcccttgacc
      121 acgcgggtcc agaaaccggg gcgctccttg gcgaccttct tcagcagatt cttgatcacc
      181 gacacttcgt ggttgtccga cggcgtgtcg acccacttgt cgccctgctc cagctcgtag
      241 cccttgtgga tcagcagcgt gacgtcgccg ccgtcatcga ccaccagctc cgggccgccg
      301 agcgtgccgt cgggcagctt gtgcgtgacc gcatcgagcg tcaggtccca gtactcttcc
      361 agcgtctcgc ccttccaggc aaacaccggc gtgccggcgg cggccactgc ggcagcggcg
      421 tcgtcctggg tcgagaagat gttgcacgag gcccagcgca ccgaggcgcc gagtgccttg
      481 agggtttcga ggagaaccgc ggtctccttg gtcatgtgca gcgagccggt caggcgcacg
      541 cccttgagcg gctgcagcgg cgcgtacttg gcgcggatgc tcatcagccc gggcatttct
      601 tcctgcgcca tgccgatgcg cttgcggccg aattcggcca gtgacatgtc cttcaccttg
      661 aagtcggtga agccagcgga tttcagaact gcgttcattt caattgctcc gatacgacgg
      721 cgctgttgat tcatggaccc tcccgccgag cctggcccgc cacatcgcgg gtcgcagcgc
      781 ctctcggcgg aaggatcgga tgctgatgct tacttcaggc cggctgcggc gcgcagctcg
      841 tcggccttgt cggtcttctc ccacgagaag gcggtgaact tcttgccgtc ggcgcccttc
      901 acttcatagg gcttgcggcc gaagtgaccg ttggaggccg tgacctggta catcgggtgg
      961 atcaggtcga gcatcttgat gatgccgtac gggcgcaggt cgaagtgcgc acgcaccagc
     1021 ttctcgatcc tgtcgtcgct gatcttgccg gtgccgaagg tggtgatgga gatcgaggtc
     1081 ggttcggcca cgccgatggc gtagctgacc tggatctcgc acttgtcggc caggccggca
     1141 gcgacgatgt tcttggccac gtagcgaccc gcataggcgg cgctgcggtc gaccttggac
     1201 ggatccttgc cggagaaggc gccgccgccg tggcgcgcgt agccgccgta cgagtcgacg
     1261 atgatcttgc ggccggtcag gccgcagtcg cccaccggac cgccgatgac aaagttgccg
     1321 gtcgggttga tgtggaactt ggtcttcttg gtgatccact tggccgggag caccggcagg
     1381 atcacctctt ccatcacgcc ttcgcgcagc ttcttcaggc tgacgtccgg cgagtgctgg
     1441 gtcgacagca cgaccgcatc gatgccgacg atcttgttgc cgtcataggc cagggtgacc
     1501 tggctcttgg cgtccgggcg cagccaggcg caaggcgagt tcttggcctt gcgcaccttg
     1561 gtctggcgct cgaccaggcg gtggctgtag tagatcggcg cgggcatcag gtccggcgtc
     1621 tcggtgcagg cgaagccgaa catcaggccc tggtcgccgg cgccctgttc ctccgggctc
     1681 ttgcggtcca cgcccatgtt gatgtcgggc gactgcttgc cgatgaagtt gagcacgccg
     1741 caggtgtggc cgtcgtaacc gacagccgag ttgtcgtagc cgatgtcggt gaccaccttg
     1801 cgcgcgatgg cttcgaaatc cgaccaggcg gtggtggtga tttcgccggc aacgacgacc
     1861 acgccggtct tgaccagggt ctcgcacgcc acgcgggctt tcttgtcctg cgccaggatg
     1921 gaatcaagga tggcatccga aatctggtcc gcgatcttgt cggggtggcc ttcggatacg
     1981 gattcggaag tgaacaggta agaggacatc ggatgcggct cctgggtgac caagcggttt
     2041 agacgatctg cctatacgga taagcggata ttatagagcc tgggccggcc gtatgcacgg
     2101 ctggggcatt ttcactgctg cgacaagatg cactacatgg ctacactggc ggccatcaat
     2161 aaaccgcgcg gatgacgccg gtaagaccaa cgggggagtg atggcattgg cggacagcca
     2221 gccggatagc gcgttgccgg tggttttcga gttgctggaa cgctcgcact accatttcca
     2281 ggccgcctgc gtggccaact acacggctgc gctgctgatg ctgctcaccg gtgcgctgat
     2341 gctgcgctgg gaacgcggca accgcaccag ccgcctgttc gccggctata ccgtgctgtt
     2401 cgctctgtgg tcggccaacc gcgccaccat gcacctgctg gacgatccgg gcctggtggt
     2461 gctgctcggg cggctgcatt actgcctggt ttccatcgcc ctgccgctgc tgctcgaatt
     2521 catcttcctg ttgctgcgca tcgaacggca gcgtcgcagc ctggtgcgct acaaccaggc
     2581 gctgggcctg ctgctggccg ccaccgcgct gcagacgccg cgggtgatct ccggcgccgc
     2641 gcattttccc tggggctacg acccgcagct cggccctgcc gggctggcca tgatggtgtg
     2701 gatcttcatg ctgatgtgcc agaccagcct ggacctgtac cgggcgcggc gccgcacccg
     2761 tccgcgcacc caggagcgcc gccgtctcaa cctgttcgtc gtcgcggtgt tcttcctgtt
     2821 tggcgcggcc aacgatttcc tggtcgacct cggcctgccg gtgtatccgc tgggcgtgct
     2881 gccggtgctg tgcttcaccc tgctcaccgg ctggctgacc tggcgctacg gcctggtgga
     2941 aatcagcgcg cagctggcgg cggacaagat cgccgagctg gcgcgcggcg cgctgctgat
     3001 gctcgacgcc gacggggtca tccagttcac caacatcagc gcgatcaaga tgctcgggca
     3061 gccgcgcaag cgcctgctcg gcagcagtgc gtcctcgctg ctgggcgatg cgttcgcggt
     3121 ggacaacctc gcccgcctgg ccgcgctgga aggacgccag gtggagaagc agctgttcta
     3181 cctgggcccg gaagagatgg tgcgcgagct ggccttctcg gtctcggcgg tgcgcgacaa
     3241 ccaccagcgc gacgtggcct atgtctgcat ggtccgcgac gtcaccgagc agaagcaggc
     3301 ggagcagcgc cgcgccgccg aaggactgaa ggacccgctg accggcctgc ccaaccgcgt
     3361 gctgttcctg gagttgctgg acaacgcggt caagcgggca cgcgagtcgc gcggctatgg
     3421 cttctgcctg ttcttcctcg gtatcgaccg catgcgcgtg atcaacgagg acctgggcta
     3481 cgccaccggc gaccgcctgc tggtggaagt ggccgcgcgc ctgcagcagc tggccggtcc
     3541 gcaggatgcg ctggcgcgcg tgggcggcga tgagttctgc ctgctggtgg agacgccgtc
     3601 gggcgcggag caggcgcgcg aactggtggc gcgcatcgag caggcggtgc gcgcgccgct
     3661 gtcgctggcc gaccacaagc tgtacctgtc ggcatcgatc ggcgtggccg ccagcgggct
     3721 ggaatacgcc agcggcgccg acatgctgcg caccgccagc atcgcgctgt accgcgccaa
     3781 ggagtccggc ggcggcggcg tgcagttctc caccgccaac gagggcggca gccagcgcac
     3841 acggctggag tccgagctcc gccgcgcggt ggaactgcag gaattctgcg tctactacca
     3901 gcccatcatc gacctgaccg aggagcgcgt cgacggcttc gaggcactgg tgcgctggcg
     3961 ccacccggag cgcggcctgc tggcgccgtc ggagttcctc gagctggccg aagaagtcgg
     4021 gctgatcgcc gccatcgaca accaggtgct gcgcaccgcc tgcgccgacc tcaaggaact
     4081 gcagcgcctg gccggcgatt tccagctgaa ggtcagcgtg aatctggccg accaggaact
     4141 gctcgacccc ggcttcaccg atcgcgtgca ggcgctgctg tcgcagcacg acctggccgc
     4201 cggcacggtc tcgctcgaac tgctcgagcg cgtggcgcag gtcgagccgg tgcgtgaaac
     4261 cctgcgccag ctgcggctga tcggcgtggg cctgtacatc gacgacttcg gcaccggctg
     4321 gtcctcgctg tcgcggctgc acgacctgcc ggccagcgtg ctcaagatcg accgcgactt
     4381 cgtgcgcgcc atgtcgctgg gcgacggcgg cgagaagatc atcagcagca ttgtcgtgct
     4441 ggcgcgcaac ctgggcatgg gcgtggtggc cgagggcgcc agcatcgccg acgaggtgcg
     4501 ccacctgcac cggctgggtt gccgctacgt gcaaggttat ttcttcgccc acgccatgcc
     4561 gttcgagggc gcggccgaac tgctgcgcga tccgcgcatg ctgcaacaga agttcagcct
     4621 gctcgatctc gacgaccagc gcatgcggcc ggtggactac cccaagcgca gcgccgaaaa
     4681 ttccggctga gttgcggaga gttcgatggc attgacgcct tccaccatgc tgccgctggg
     4741 cacgttggcc ccgccgtttt ccctgcccga cgtggtcagc ggcaacacgc tggagctggc
     4801 gcggctgaag tccgcgcagg ccacgctggt catgttcatc tgcaaccact gtccgtacgt
     4861 gaagcacgtg cgcgcgcagc tggtgcagct ggcgcgggac taccagccgc gcggcgccgc
     4921 catcatcgcc atcagcagca acgatgcgca gaaatacccg gaggatgcgc cggcacgcat
     4981 gcgcgagacc gcgctggagc accactaccc attcgtgtac ctgtacgacg agacgcagga
     5041 ggtggcgcgt gcctaccgcg ccgcctgcac gcccgatttt ttcctgttcg acggcgcgct
     5101 gaagctcgcg taccgagggc agctcgacga ttcgcgcccc ggcaacggca agccggtgac
     5161 cggccgcgat atgcgcagtg cactggatgc gcttctggcc ggacgcgcgc catcccccga
     5221 gcagcagccg agcgtgggtt gcaacatcaa gttccgctga ggagattgca tatgaagaag
     5281 tcacagggat gctggctgct gatcgcactg ggcgcagtga cggcgaacgc ttacgccgat
     5341 ggctgcggca aggagcgcgc gctcaatgcg gccgaacaga aattcgtcgc cgatcacctg
     5401 gccgcggcgc gcgcggcatt tcccgcgccg cccgcgggct gggcgcgcta cgagccggat
     5461 gccgggctgc gggcgcgcgt ggcgcaggac ctgccgcccc cgacaaccgt gtgcgagcaa
     5521 tgggggccgc gcccgctgag cctgtcggcg cacgcgacct atacaccggc ggattcggcg
     5581 ctgatgcgcg acctgcagaa gcagcgcgac gagcaggcgc agcagaagaa ggtggcggag
     5641 ctgcagcaga agatgcagga ggccggtgcg cgcggtgact acaacgccat ggcctcgctc
     5701 aaccagcaga tgcaggcggc ctcgatgccg gccggggcca aggcggcggc gccgatgtcc
     5761 gacgccgaat acgaccgcca gaacgaagcg ctgtcgaaga agcttgaggc cgcgcaggcc
     5821 aggagcgact acacggcgat gcagcagatc agcgcgcagc agatggcgct ggtcatgcgc
     5881 aaggccggcg ttgacaccag cggcgtggcg gcggcggaaa tcaccagcga ccagatcaat
     5941 gcagcgccgc gcgtgcacgt ggtggtcgag ttcaaccgcg acgcgttcga catcccggag
     6001 aacgcgaaga aggtgagcta cggtggcgcg gcggtgggct acgccgcgcg cggcgatcac
     6061 ttctgctacg acaacggcgg cggcagcgac cgcgagcagg tctacctgct gttcggcccg
     6121 tggcagggca agaccaacta caacaaccag ctggcgaact acatctcaga cgccgccgac
     6181 cacggcgcgt ttgcctcgca ggtcaccacg ctggtgtcga tctgcggcga cccggcgatg
     6241 gtggagaagc tggcgccggc catcagatac gacgcgctgc gccgctagc
//
LOCUS       DASZZC010000610         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_498374,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000610 DASZZC010000000
VERSION     DASZZC010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498374"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /gene="secG"
                     /locus_tag="VHE37_13045"
     CDS             complement(<1..172)
                     /gene="secG"
                     /locus_tag="VHE37_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HVT36507.1"
                     /translation="MYTVLVVVQVLVAISLIVLILLQHGKGADAGAAFGSGASGTVFG
                     ARGAANFLSRTTA"
     gene            complement(191..961)
                     /gene="tpiA"
                     /locus_tag="VHE37_13050"
     CDS             complement(191..961)
                     /gene="tpiA"
                     /locus_tag="VHE37_13050"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVT36508.1"
                     /translation="MARQYLVAGNWKMNGSSDANAKLVRDLLFDVKKYPDVEVVVCPP
                     MLYVESVGKLIAGSKIGLGAQTLCEQEKQGAYTGEVHGGMLKDLDCQYVIVGHSERRA
                     YYGETDALVARKFATAQACGLKPILCIGETLAEREGNQTEAVLARQLNAVIAACGILS
                     FKTAVIAYEPVWAIGTGKTASPEQAQAAHAHIRGLLSANDAKIAASTRILYGGSVKAD
                     NAQTLFACADVDGGLIGGASLKAAEFLAICAGAQAQVA"
     gene            complement(987..2324)
                     /gene="glmM"
                     /locus_tag="VHE37_13055"
     CDS             complement(987..2324)
                     /gene="glmM"
                     /locus_tag="VHE37_13055"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HVT36509.1"
                     /translation="MSQKYFGTDGIRGRVGQAPMTPEFALKLGWAAGRVLGKTPGTRV
                     VIGKDTRRSGYVFESALESGFASAGVEVLLLGPLPTPGVAYLTRALRAQAGVVISASH
                     NPHHDNGVKFFDGEGGKLSDSIELEIEAMLEQPLQCVEPQELGRAKRINDASGRYIEF
                     CKGSFGEKNLRGVKIVVDCAHGAAYRTAPAVFEELGADIDVIADRPDGLNINDGCGST
                     HLEPLVKAVKKAKADIGIALDGDADRCLLVDADGNTIDGDEILYIIARGRLAAGGLKG
                     PVVGTLMTNLGLERALVEMKLGFERARVGDRYVLEMLKAKGGVLGGETSGHTICLDKT
                     TTGDGTVTALQVLSAMVQQGQPLKQLAAGMHKYPQVLINVPVKGKAAPILQSAALKAA
                     EEASRQRLGEHGRLLLRGSGTEPIIRVMVEAQDAAQTRREAELLADAVRSVVA"
BASE COUNT          389 a          820 c          737 g          437 t
ORIGIN      
        1 aggcggtggt gcgcgaaagg aaattggccg cgccgcgtgc gccgaacacg gtgccggaag
       61 cgccgctgcc gaatgccgcg cccgcgtccg cacctttgcc atgctgcagc aggatcagca
      121 cgatcagcga gatcgccacc agcacctgca ccaccaccag aactgtgtac atgtccgaac
      181 cagaaataag ttaagccacc tgtgcctgcg cgcctgcgca gatcgccagg aattccgctg
      241 ccttgaggga agcgccgcct atcagcccgc catccacgtc ggcacaggcg aacaaagtct
      301 gcgcattgtc ggccttgacg ctgccgccgt agaggatgcg cgtggatgcg gcaattttag
      361 catcattcgc cgacagcaga ccgcggatgt gggcgtgtgc agcctgggcc tgctcgggcg
      421 aggcggtctt cccggtgcca atggcccaga ccggctcata agcaatcacc gctgtcttga
      481 acgacaggat gccgcaggcg gcaatcaccg cattgagctg gcgcgccagc accgcttccg
      541 tctggttgcc ttcgcgttcg gccagggtct cgccaatgca caggatgggc ttgagaccgc
      601 aggcctgggc ggtggcgaac ttgcgtgcga ccagcgcatc ggtctcgccg taatatgcgc
      661 ggcgctcgga atggccgacg atgacgtact ggcagtccag gtccttgagc atgccgccat
      721 ggacctcgcc ggtatatgcg ccctgctttt cctgttcgca cagcgtctgc gcacccaggc
      781 cgatcttgct gccggcgatg agcttgccga ccgactcgac gtacagcatg ggcgggcata
      841 ccaccacttc gacgtcgggg tatttcttca cgtcgaacag caggtcgcgc accagcttgg
      901 cgttggcgtc gctgctgccg ttcatcttcc agttccccgc caccagatat tgccgcgcca
      961 tctgtgctcc cctgttcgct ttcgaattac gcaaccacgc tgcgcacggc atcggccagc
     1021 aactcggctt cacgccgggt ctgcgcggca tcctgtgcct ccaccatcac gcggatgatc
     1081 ggctcggtgc cggacccgcg cagcagcagc cggccgtgct cgcccaggcg ctggcgactg
     1141 gcttcctcgg cggccttgag cgccgctgac tgcaggatcg gagcggcctt gcccttgacc
     1201 ggcacgttga tcagcacctg cggatacttg tgcatgccgg ccgccagctg tttcagcggc
     1261 tgtccctgct gcaccatggc cgacagcacc tgcagcgcag tgacggtacc gtcgccggtg
     1321 gtagtcttgt ccaggcagat tgtgtggccg gaggtttcgc cgccaagcac gccacccttg
     1381 gccttcaaca tttccagcac gtagcgatca cccacgcgtg cgcgctcgaa gcccagcttc
     1441 atctccacca gcgcacgctc cagcccgagg ttggtcatca gcgtgccgac aaccggcccc
     1501 ttcagaccgc ctgcagcgag gcggccgcgc gcgatgatgt agaggatctc gtccccatcg
     1561 atggtattgc catcggcatc gaccagcagg cagcggtcgg catcaccgtc gagcgcaatg
     1621 ccgatatcag ccttggcttt cttgaccgcc ttcaccaggg gttcgaggtg cgtggagccg
     1681 caaccgtcgt tgatgttgag cccgtccggg cggtcagcga tgacgtcgat gtccgcgccc
     1741 agttcttcga atactgccgg cgccgttctg taggcggcgc catgcgcaca gtccaccacg
     1801 attttcacgc cgcgcaggtt cttctcgccg aagctgccct tgcagaattc gatgtagcgg
     1861 ccggaagcgt cgttgatgcg cttggcgcgg cccagttcct ggggctctac gcactgcaac
     1921 ggctgctcga gcatggcttc gatttccagc tcgatactgt ccgacagctt gccgccttcg
     1981 ccgtcgaaga acttcactcc attgtcgtga tgcgggttgt gcgaggcgga aatcaccacg
     2041 ccggcctgcg cacgcagcgc gcgcgtgaga taggcaacgc cgggtgtcgg cagcgggccg
     2101 agcagcagca cttcgacgcc ggcactggcg aaccccgact ccagcgcgga ttcgaacacg
     2161 tagcccgagc ggcgcgtgtc cttgccaatg accacacggg tgccgggcgt cttgccgagc
     2221 acgcggccgg ccgcccagcc gagtttcagc gcgaactccg gcgtcatcgg cgcctggccg
     2281 acgcgtccgc ggatgccgtc cgtgccgaaa tacttttgac tcaccccgat ctccgttgcg
     2341 ccgcgacggc ggccgccagc ttgatggcct cgacggtggc gcg
//
LOCUS       DASZZC010000611         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_745041,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000611 DASZZC010000000
VERSION     DASZZC010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745041"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1572)
                     /locus_tag="VHE37_13060"
     CDS             complement(154..1572)
                     /locus_tag="VHE37_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT36510.1"
                     /translation="MKAGMTLRSMTLGLLLAAAAQAGATATADEAAKLGGELTPVGAT
                     AAGNKDGTLPEWTGPKNFPDEMKAWTPKVLEDMRKQDPAKLEAEFRKFLSEDVQKVVL
                     TITPQNMAQYADKLTEGQKAMLKAYPDYKIPVYKTVRTAYYPDEIYKWTRINATKAHL
                     EGTDSVKDAHLGFPFPIPKSGAEVIWNHKLKFRGSAARRYNNQAIVKADGSYVITKLI
                     EDVKFKYGNLGDPADLAKSPVMLYYLSETISPPRVAGQMTLVHESADASEGGRLAWIY
                     QPGLGRVNRAPDVGYDNPAVGTDNEQYNDQVDVFNGALDRYNWKLVGKKEMYIPYNSL
                     QINSPRVKYKDILRPYHINQDLARYELHRVWIVEATLKPGLRHNFARRVFYVDEDSWS
                     IAAVDCYDARDQLWKVQEAHLITAPFVPTVTGVPELIYDLQSKRYFATAMQNEDNITD
                     FKIDFPDSYFDPANLKRKARSK"
     gene            complement(1674..>1829)
                     /locus_tag="VHE37_13065"
     CDS             complement(1674..>1829)
                     /locus_tag="VHE37_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVT36511.1"
                     /translation="LLATTTEPIENIVYLTGFSEPSTFYRAFKRWSGMTPVQYREARS
                     SAQAGPI"
BASE COUNT          269 a          553 c          611 g          396 t
ORIGIN      
        1 aaaagcaaaa cccctctcgc ggcgggagag gggcaggggt gagggacggg ccctcatccg
       61 gcgctgcgcg cgcgtttggc ttaacgcaac gtgacgttta gtcacgctgc gtccgggcca
      121 aacgctctcc cggcgggaga aggaaaccgc ttttcatttg ctgcgcgcct tgcgcttgag
      181 gtttgcagga tcgaaatagc tgtccgggaa atcgatcttg aagtcggtga tgttgtcctc
      241 gttctgcatc gcggtggcga agtagcgctt gctctgcagg tcgtagatca gctccggcac
      301 gccggtcacc gtgggcacga agggcgcggt gatcaggtgc gcttcctgca ccttccacag
      361 ctgatcgcgg gcgtcgtagc agtccaccgc ggcgatcgac cagctgtcct cgtcgacgta
      421 gaacacgcgc ctggcgaagt tgtggcgcag gccgggcttg agcgtggcct cgacgatcca
      481 cacgcggtgc agttcgtagc gggccaggtc ctggttgatg tggtacggcc gcaggatgtc
      541 cttgtatttc acgcgcgggc tgttgatctg cagcgagttg tacgggatgt acatctcctt
      601 cttgccgacc agcttccagt tgtagcggtc gagcgcgccg ttgaacacgt cgacctggtc
      661 gttgtactgc tcgttgtcgg tgcccaccgc cgggttgtcg tagcccacgt cgggcgcgcg
      721 gttcacgcga ccgaggccgg gctggtagat ccacgccagg cggccgcctt cgctggcgtc
      781 ggcgctttcg tgcaccagcg tcatctggcc ggccacgcgc ggcggcgaga tggtttccga
      841 gaggtagtag agcatcaccg gcgacttcgc caggtcggcc ggatcgccga ggttgccgta
      901 cttgaacttc acgtcctcga tcagcttggt gatgacatag ctgccgtcgg ccttgacgat
      961 ggcctggttg ttgtaacgcc gcgccgcgct gccgcggaat ttcagcttgt ggttccagat
     1021 gacttcagcg ccggatttcg ggatcgggaa gggaaaaccc aggtgcgcgt ccttcaccga
     1081 gtcggtgcct tccaggtgcg ccttggtggc attgatcctg gtccacttgt agatctcgtc
     1141 cgggtagtac gcggtgcgca ccgtcttgta gaccgggatc ttgtagtcgg ggtaggcctt
     1201 gagcatcgcc ttctggcctt cggtcagctt gtcggcgtac tgcgccatgt tctgcggcgt
     1261 gatggtgagg accaccttct gcacgtcttc gctgaggaac ttgcggaact cggcttcgag
     1321 cttggccgga tcctgcttgc gcatgtcctc gagaaccttc ggcgtccatg ccttcatctc
     1381 gtcggggaaa ttcttcgggc cggtccattc cggcagcgtg ccgtccttgt tgccggcggc
     1441 ggtggcgccg accggcgtca gctcgccgcc gagcttggcc gcttcatcgg cggtggccgt
     1501 cgcgccggcc tgtgctgctg ctgcgagcag caagccgagc gtcatgctcc tgagcgtcat
     1561 gccagccttc atagcgtctc tcctgaagtc ccttgaggtt cgtgtaatcg gcggtcgcgc
     1621 aatctaaagc cgtggcaagt ggggcgccat gtcagcgcat gccagtccag ccgtcaaatc
     1681 gggccagcct gcgcggaact cctggcctcg cggtactgca ccggcgtcat gccgctccag
     1741 cgcttgaacg cacggtagaa ggtgctgggc tcggagaagc cggtgagata gacgatgttc
     1801 tcgatcggct ccgtggtcgt cgccagcag
//
LOCUS       DASZZC010000612         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1204286,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000612 DASZZC010000000
VERSION     DASZZC010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204286"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VHE37_13070"
     CDS             <1..435
                     /locus_tag="VHE37_13070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVT36512.1"
                     /translation="QRDSGEKFIDNRYSRRHQWSFDGGISVPASSSPQHVPEPYSAAD
                     AVDPEEAFVAALSSCHMLFFLYHAARKKWTVERYEDDALGLMGKNAQGQDAIVRVTLR
                     PRVSFSGTVPGADEVNALHEQSHHDCYLANSVRSEVLIEPRV"
     gene            complement(443..2458)
                     /locus_tag="VHE37_13075"
     CDS             complement(443..2458)
                     /locus_tag="VHE37_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36513.1"
                     /translation="MAYTPQYPRSALAVILLLVLSCVALMAVAGHKYTHITGINNIVE
                     DARGRLLVNLNDGLVRLTADGALETHFNFGDRTLGGRHTAFAPMPDGRWWLGNYDKGT
                     LQLMSEQGTLIRSVAPPASAGPFFGAFHLGYEPKSRRLIVSDSQNHRLLAFDAEGHFI
                     GVSARGMNLRYPTGLAALPQGTLLLANMNRHLAMTINPDLTEAGLRFEGGNLTYRYSE
                     FVSAAADGRAYVTWHDHNLEYGAIAAYAADGHFLHVLPLGDKAMPLTVAARSDDVLAA
                     WKTDDRFSLHHFDSTGRDLGEFGDTHLQSFLRIASDDKVRWRRIRELSKLGAMLGAIG
                     LLLVALRLRRQQEKTAAASVMPPVPAAPAGQTMSIAFGVVGAVLLLALVQTIGCSTIL
                     ANSLASHGQYHPLEHSAVWITALLWIVIATAWRSSTVFLQLRATMARRAMVAWAPYLP
                     TIMEPGESVLDYESCARYLSAYLLVATNRRLIQLPFEAGEAIKPGWEASWQQRIQAVV
                     LPRPWWLALLNPVGKLMELRASGGRQSARFFAISQTHSEKIIALVRTAREQALQQNPN
                     LPPLAAAVPRIANRLQPSLWRSLLLSLVLPGLGQLNQGRTRRGTVLMSFAAVLLLPTS
                     INWFIAIRRIADQGDGPLYFSAIVYAALVAANLWDVVQFDRRLRTTG"
     gene            complement(2882..>3997)
                     /locus_tag="VHE37_13080"
     CDS             complement(2882..>3997)
                     /locus_tag="VHE37_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT36514.1"
                     /translation="RVFDYFDYVCLDAGERPLLALLEHLRGARPRERLVRTYARNGRG
                     AVQYLDCGEPDIPFAQIGTPTWDGLPLDGYLSVLDLLNPMHRLWSDGRWNKLTVAHGC
                     YWKKCSFCDVSLDYISRYDTAGAAILCDRIDAIVAETGQSGFHFVDEAAPPKALKTLA
                     AELLRRGRSISWWGNIRFEKTFTPELCRLLADSGCIAISGGLEVASDRLLRLMKKGVS
                     VAQVARVTRAFADAGILVHAYLMYGFPTQTVQDTVDALEYVRQLFNAGCIHSGFFHRF
                     ACTVHSPVGLHPQQYGVTLLPPAEGKFARNDIGFIDPTGVDHDALGRALNQALYNYMH
                     GIGLDQDVRRWFDGRVPRSTVPKHFIEQACAPASAAA"
BASE COUNT          742 a         1242 c         1321 g          692 t
ORIGIN      
        1 caacgcgact ccggcgagaa attcatcgac aaccgctatt cgcggcgcca ccagtggagc
       61 ttcgacggcg gcatcagcgt gccggcgtca tcctcgccgc agcacgtgcc ggagccgtac
      121 tcggcggccg acgcggtcga cccggaagaa gccttcgtcg ccgccctctc gtcgtgccac
      181 atgttgttct tcctgtacca cgcggcgcga aaaaaatgga cggtcgagcg ctacgaggac
      241 gacgcgctcg ggttgatggg caagaacgcg caggggcagg acgccatcgt gcgcgtcacg
      301 ttgcgtccgc gcgtgagctt ttccggcacg gtgcccggcg ccgatgaagt gaacgcgctg
      361 cacgagcagt cgcaccacga ctgctacctg gccaactccg tgcgcagcga ggtgctgatc
      421 gagccgcgcg tctgaggcgc cttcaaccgg tagtccggag tctgcgatca aactgcacga
      481 catcccataa attcgctgcg accagcgcgg catagacgat cgctgagaaa tacagtggcc
      541 cgtcgccctg atcagcaatg cggcggatgg cgatgaacca attgatcgaa gtgggcagca
      601 gcagcacggc agcgaaggac atcagcacgg tgccgcggcg cgtgcggccc tggttcaact
      661 ggcccaggcc gggaagcacc agcgatagca ggaggcttcg ccaaagagaa ggctgtagcc
      721 gatttgcaat tcgtggcacg gctgcggcaa gcggcggcag gttcgggttc tgctgcagcg
      781 cttgttcgcg ggcggtccgt acgagggcaa tgatcttctc ggaatgtgtt tgactgatgg
      841 caaagaagcg tgcggattgc cggcccccgc tcgccctcag ttccatgagc ttgccgacgg
      901 gattgagcag cgccagccac catggcctcg gcaggacaac cgcctgtatt cgctgctgcc
      961 agctggcttc ccatcccggt tttatggcct ctccggcttc aaacggcagc tgtatcaggc
     1021 gccggtttgt tgcgaccagc aaataggcgg agagatacct ggcgcaactc tcgtagtcga
     1081 gtaccgactc gccaggttcc atgatggtgg gcagataggg agcccaggcc accatggcac
     1141 ggcgcgccat ggtggcacgc aattgcagga acacggtgct ggatctccag gcggtggcaa
     1201 tgacaatcca cagcagcgcg gtgatccaga ctgcgctgtg ctccagcgga tggtattgcc
     1261 cgtgtgatgc cagggagttg gcgagaatag tcgagcagcc aatcgtctgc accaatgcca
     1321 acagcagcac ggccccgacg actccaaaag cgatcgacat ggtctgccct gctggcgccg
     1381 cgggcacggg cggcatgacg ctcgcggcgg cggtcttctc ctgctgccgc cgcagacgca
     1441 gcgcaaccag cagcaagcca atcgcgccta gcattgcacc gagttttgac agctcgcgaa
     1501 tccggcgcca gcgcactttg tcgtccgacg caatgcgcaa gaacgactgc aggtgggtat
     1561 cgccgaattc accgaggtca cggccggtgc tgtcgaaatg gtgcaggctg aaacggtcat
     1621 cggttttcca tgccgccagc acatcatccg atctagcggc caccgtgagc ggcatggcct
     1681 tgtcgcccag cggcagcaca tgcagaaaat ggccgtctgc tgcataggcg gcaatcgcgc
     1741 cgtactccag attgtggtcg tgccaggtaa cgtaggcgcg gccatccgca gcggcagaga
     1801 caaattcgga ataccggtag gtcaaattgc cgccttcaaa cctcagccca gcctcggtaa
     1861 ggtctgggtt gatcgtcatg gcaagatgac gattcatgtt ggccagcagc aaggtgcctt
     1921 gaggcagcgc ggcaagtccg gttgggtagc gcaggttcat gccgcgtgcg ctaacaccga
     1981 tgaaatgtcc ctcggcatcg aaggcaagca gacgatgatt ctgcgaatcg gacacgatca
     2041 atcgccgtga ctttggctcg tagcccaagt ggaacgcgcc gaagaatggt cctgcggatg
     2101 ctggcggagc caccgacctg atcagggttc cttgctcgga catcagttga agcgtgccct
     2161 tgtcgtagtt gcccagccac cagcggccat ctggcatcgg tgcaaacgcg gtatggcggc
     2221 cgccgagtgt ccgatcacca aaattgaagt gcgtttccag cgcgccatcg gcagtgaggc
     2281 gcaccaagcc atcattgaga tttacgagga ggcggccgcg agcgtcttcc acgatgttgt
     2341 tgatgccggt gatgtgggtg tacttgtggc cggccactgc catcaatgcc acgcagctca
     2401 acaccagcag caatatgacg gccaaggccg atcgcggata ctgaggcgtg tacgccatgg
     2461 ttttccccct tgctgcattg cccggtccgt gtagccggca cgcaatatga gcagaagact
     2521 agcgacgttt tgccgtgggg gccattggtt atttcagcaa tgttttggtg cgcgcggcat
     2581 gtctccgcct gatgacgcgt aagtttcagg ccagcgcgcc gtgccgtggc gccgggtcag
     2641 cgccgatccg ccgcggggtc caccgcggcg cgcgcatggg cctgcagccc gtacgcggag
     2701 taggtgagag agagcacggt gggcagaggt tccagcggtc ttgcgcgaga tgcgcacttc
     2761 gccgatcacg tcggccacgg aaaatccggc gtagcctttc ttcaccaccg cccgccccag
     2821 aacgcggatg tctcggcgcc cgcgctcctg cctgccttgc gggccatggc ggtctgcgcg
     2881 gtcaggccgc ggcggatgcc ggcgcgcagg cctgctcgat gaaatgcttg ggcaccgtgc
     2941 tgcgcggcac gcggccgtcg aaccagcggc gcacgtcctg gtccagcccg atgccatgca
     3001 tgtagttgta cagcgcctgg ttcagcgcac ggccgagcgc gtcgtggtcc acgccggtgg
     3061 ggtcgatgaa gccgatgtcg ttgcgcgcaa attttccttc cgctggcggc agcagcgtta
     3121 cgccgtactg ctgcggatgt agtccgaccg gcgaatgcac ggtgcaggcg aagcggtgga
     3181 agaagccgga gtggatgcag ccggcgttga acagctggcg cacgtattcc agcgcatcaa
     3241 ccgtgtcctg caccgtctgc gtggggaaac cgtacatgag gtacgcgtgc accaggatgc
     3301 cggcgtcggc gaaggcgcgc gtgacgcgcg ccacctgcgc caccgacacg cctttcttca
     3361 tcagccgcag caggcggtcg gaggcgacct ccagcccgcc ggagatggcg atgcagccgc
     3421 tgtcggccag caatcggcac agctccggcg tgaaggtttt ctcgaagcgg atgttgcccc
     3481 accagctgat cgaacggccg cggcgcagca gttccgccgc gagcgtcttc agcgccttgg
     3541 gcggcgcggc ctcgtcgacg aagtggaaac cgctctggcc ggtctcggcg acgatggcgt
     3601 caatgcggtc gcacaggatc gccgcgcccg cggtgtcgta acgcgagatg taatcgaggc
     3661 tcacatcgca gaagctgcat ttcttccagt agcagccatg cgccacggtg agcttgttcc
     3721 agcggccgtc tgaccacagg cggtgcatcg gattgagcag gtcgagcacg gagaggtagc
     3781 cgtcgagcgg cagcccgtcc caggtgggcg tgccgatctg cgcgaagggg atgtccggct
     3841 cgccgcagtc gaggtactgc accgcgccgc gcccattacg tgcataggtg cgtaccaggc
     3901 gctcgcgcgg gcgcgcgccg cgcaggtgtt cgagcagcgc gagcagaggg cgctcgccgg
     3961 cgtcgagaca cacgtagtcg aagtagtcga acacgcg
//
LOCUS       DASZZC010000613         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_243700,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000613 DASZZC010000000
VERSION     DASZZC010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243700"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="VHE37_13085"
     CDS             complement(<1..1861)
                     /locus_tag="VHE37_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT36515.1"
                     /translation="MISLTNATLRRGARTLLENVSFTIFSGQHVGIVGKNGTGKSSLF
                     QLLLGQLHTDAGDASMPKNLAIATVAQETPGLPVPALEYALDGDVELRQLERDLAVAE
                     EKHDGDAISHIHERLLQIDGYAARARAAQLLRGLGFAPDVQENAVESFSGGWRMRLNL
                     ARALMCRSDLMLLDEPTNHLDLDAVIWLAEWLGNYPGTLLLISHDREFLDSITTHTLH
                     LENQTATLYAGNYSQFERQRAERMTQQAARYEKQERERAHLQSFIDRFKTHASKARQA
                     QSRVKALERMELIAPAHWDSEFSFSFREPERVPSPLLTFDDVAAGYGDARIVERVRLS
                     LEPTDRIGLLGPNGAGKSTLMRTLAGVQEPLAGDTHRDPYLKIGYFAQHQLEQLDAKA
                     SPLLHLKRISPRSYEQDLRDFLGSFGFNGDRVSEAVAPFSGGEKARLALALIVFQRPN
                     LLLLDEPTNHLDLEMRYAVEAALAEFKGAVIMVSHDRHMLTSCCNEFWRVAEGRCEKF
                     DGDLDDYARWLQQRLKADPAAKESTPRPQKMSARDQRKAGADARAAQAALKKLEAKLE
                     KLGAELKSVMDALGDEALYAPERKKDLSELLNRQSQLKRQLAQAEEEWLEKSEG"
BASE COUNT          304 a          656 c          689 g          396 t
ORIGIN      
        1 tgccctcgga tttttccagc cattcctctt cggcctgcgc cagctgccgc ttgagctgcg
       61 actgccggtt gagcagttcg gacaggtcct tcttgcgctc gggcgcgtac agcgcttcgt
      121 cgcccagcgc atccatcacg ctcttgagtt cggcgccgag cttttccagt ttcgcttcga
      181 gcttcttcag cgcggcctgt gcggcgcgcg catcggcgcc cgccttgcgc tgatccctgg
      241 ccgacatttt ctgcggcctc ggcgtggatt cctttgcggc cggatcggcc ttcagccgct
      301 gctgcagcca gcgcgcgtag tcgtcgaggt cgccgtcgaa tttctcgcag cggccttcgg
      361 ccacgcgcca gaactcgttg cagcagctgg tcagcatgtg gcggtcgtgc gagaccatga
      421 tgaccgcgcc cttgaactcg gccagcgccg cttccacggc gtagcgcatc tcgaggtcaa
      481 ggtggttggt cggctcgtcg agcagcagca ggttcggacg ctggaacacg atcagcgcca
      541 gcgccaggcg cgccttctcg ccgccggaaa acggtgcgac ggcttcgctc acgcggtcgc
      601 cgttgaagcc gaagctgccg agaaagtcgc gcaggtcctg ctcgtacgaa cgcgggctga
      661 tgcgcttgag gtgcagcagc ggcgaggcct tggcgtcgag ctgttccagc tggtgctggg
      721 cgaagtagcc gatcttcaaa tacggatcgc ggtgcgtgtc gccggcgagc ggctcctgca
      781 cgcccgccag cgtgcgcatc agcgtggatt tgcccgcgcc gttggggccg agcaggccga
      841 tgcggtcggt cggttccagc gacagccgca cacgctcgac gatgcgcgcg tcgccgtagc
      901 cggcggcaac gtcgtcgaag gtgagcagcg gcgagggcac gcgctccggc tcgcggaagg
      961 agaaggagaa ctcgctgtcc cagtgcgccg gcgcaatcag ctccatgcgc tccagcgcct
     1021 tgacgcgcga ctgcgcctgg cgcgccttgc tcgcgtgcgt cttgaagcgg tcgatgaacg
     1081 attgcaggtg cgcgcgctcg cgctcctgct tctcgtagcg cgccgcctgc tgtgtcatgc
     1141 gctcggcgcg ctggcgttcg aactgcgagt agttgccggc gtacagcgtc gccgtctggt
     1201 tttccaggtg cagcgtgtgc gtggtgatgc tgtcgagaaa ttcgcggtcg tgcgagatca
     1261 gcagcagcgt gcccgggtag ttgcccagcc attccgccag ccagatcacc gcgtccaggt
     1321 cgaggtggtt ggtcggctcg tcgagcagca tcaggtcgct gcggcacatc agcgcgcgcg
     1381 ccaggttcag gcgcatgcgc cagccgccgg aaaacgattc caccgcgttc tcctgcacgt
     1441 ccggcgcgaa gccgagaccg cgcaagagct gcgccgcgcg cgcgcgcgcc gcgtagccgt
     1501 cgatctgcag caggcgctcg tggatgtgcg agatggcgtc gccatcgtgc ttctcttcgg
     1561 cgaccgccag atcgcgttcg agctggcgca gttccacgtc gccgtccagc gcgtactcga
     1621 gcgccggcac cggcaggccc ggtgtttcct gcgcgacggt ggcgatggcc aggttcttcg
     1681 gcatgctggc gtcgccggcg tcggtgtgca gctggccgag cagcaattga aacaggctcg
     1741 acttgccggt gccgttcttg ccgacgatgc caacatgctg gccggagaaa atggtgaagc
     1801 tcacgttctc cagcagcgtg cgcgcgccgc ggcgcagggt ggcgttggtc agggagatca
     1861 tgcggcggcg ctcatggcgg cgcgcagtct actggcattc agtggccaaa accgcctcca
     1921 ccgcatgcca ttttttgatg ttataaaata ttgttcaaat ataaatatat acaataatta
     1981 ataacatcaa caatgccggt gcggtggagg tgttttttgc aggagcgcgc cttgcgcgcg
     2041 atgtg
//
LOCUS       DASZZC010000614         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1265884,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000614 DASZZC010000000
VERSION     DASZZC010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265884"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1253)
                     /gene="lldD"
                     /locus_tag="VHE37_13090"
     CDS             complement(102..1253)
                     /gene="lldD"
                     /locus_tag="VHE37_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014719250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004457 - lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HVT36516.1"
                     /translation="MKAAAVIDYRELARRRLPHFLFEYIDGGSYDEATLRRNLSDLSA
                     IALRQRVLRDVSQLDLSVELFGQKLSLPVALAPIGLAGLNARRGEVQAARAAQRAGVP
                     FCLSTVSACPLAEVSAGVTQPFWFQLYMIRDRGFMRDLLAQAEAARCSALVFTVDMPV
                     PGSRYRDYRSGLAGAPGIGGALRRFAQAALRPAWAWDVGVRGRPHALGNVAPVLGENT
                     GLEDFFAWMRNNFDPSVTWKDMEWVRSQWRGPLIVKGILDAEDARAAASLGADGIVVS
                     NHGGRQLDGVLSTARALPPIADAVGDKLTVLADSGVRSGLDVVRLLALGARGVRLGRA
                     WAYALAARGEAGVAHVLELMAAEMRVAMALTGCVRVAEINRSILAGGGA"
     gene            complement(1250..2446)
                     /locus_tag="VHE37_13095"
     CDS             complement(1250..2446)
                     /locus_tag="VHE37_13095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HVT36517.1"
                     /translation="MNDNTHGLDLRTPLRYTIVTFFIWGLAYGLLDVLNKHFQEILHV
                     GKAQSAWLQIAYFGAYLTMSIPAGMWMHARGYKVGILTGLTVAAGGAFLFIPAASAMS
                     FPFFVGAMFVLATGLCFLETSADTYVNVLGAPEHAERRLNLAQSFTGLGGFLGPMIGG
                     AVFFGGTAAAGEELHSVQLTYGVIGALILLYAVFIARAQLPEIQAATITDTGASRPLS
                     TRSHFIFGVITQALYVGAQVGIGAYFINLVTESWKGLSSQEGAFLLSVATLLYLVGRF
                     LSTALMTRFDPRKILTTYGLANVALTLIVAAGIDKVSAIALVGVFFFMSAMFATTFTL
                     GIKDLGAQTKRGSSFMVMAIGGGVLLPYPMGMIAESYGSPAAFLLPCACFALVAWYGW
                     RGAEVR"
     gene            complement(2540..>2831)
                     /locus_tag="VHE37_13100"
     CDS             complement(2540..>2831)
                     /locus_tag="VHE37_13100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT36518.1"
                     /translation="KRGMALLAKQPNVAVKISGLAMIDRTWTTDSIRPFVLDTIDLFG
                     PQRAMFASNFPVDKLYGNFGQHFEAYSSIVADFSDAERRALFAGTAETIYRI"
BASE COUNT          512 a          959 c          946 g          414 t
ORIGIN      
        1 catctgcgcc agcttgatgg aaggcggccc tggcgcactc cgcgcgtcgt cttgccgcaa
       61 cagcaattgc aatggggccg ctatcgcggc gcagatgcaa tttacgctcc gccgccagcc
      121 agaatactgc gattgatctc cgccacgcgc acgcagccgg tgagcgccat ggccacgcgc
      181 atctccgccg ccatcagttc cagcacatgg gccaccccgg cctcgccgcg cgcggcgagc
      241 gcataagccc aggcccggcc gagccgcacg ccgcgggcgc ccagcgcgag caggcgcacc
      301 acgtcgaggc ccgagcgcac gccgctgtcc gcgagcacgg tgagcttgtc gccgaccgca
      361 tcggcgatgg gtggcagggc gcgcgcagtg gacagcacgc cgtcgagctg gcggccgccg
      421 tgattcgata cgacgatgcc atcggcgcct agcgacgcgg cagcgcgcgc gtcttcggcg
      481 tcgaggatgc ccttgacgat gagcggtccg cgccattggc tgcgcaccca ctccatgtcc
      541 ttccaggtca cggatggatc gaagttgttg cgcatccagg cgaagaaatc ttccaggccc
      601 gtgttctcgc ccagcaccgg tgccacgttg cccagcgcat gcgggcggcc gcgcacgccc
      661 acgtcccagg cccaggccgg gcgcagcgcg gcctgcgcga agcggcgcag cgcgccgccg
      721 attcccggcg cacccgcgag gccggagcga tagtcgcggt agcgcgagcc gggcaccggc
      781 atgtccacgg tgaagaccag tgcgctgcag cgtgccgctt ccgcctgcgc cagcaggtca
      841 cgcatgaagc cgcggtcgcg gatcatgtac agctggaacc agaatggctg cgtcacgccg
      901 gcgctcacct ccgccagagg gcaggccgag accgtggaca ggcagaacgg cacgccagcc
      961 cgctgcgcgg cgcgcgcggc ctgcacttcg ccgcggcgtg cgttgaggcc agccaagccg
     1021 atcggcgcca gcgcgaccgg cagggaaagt ttctgtccga acagttcgac cgacaggtcc
     1081 agttgcgata catcgcgcag cacgcgctgg cgcagcgcaa tggccgagag gtcagagaga
     1141 ttgcgccgca gcgtggcctc gtcgtacgag ccgccgtcga tgtactcgaa caggaagtgc
     1201 ggcaggcgcc gccgcgccag ctcgcggtag tcgatgaccg ccgcggcctt catcgcactt
     1261 cggccccgcg ccagccgtac caggcgacca gcgcgaagca cgcgcacggc aacaggaacg
     1321 ccgcgggcga gccgtagctt tccgcgatca tgcccatcgg gtagggcagc agcacgccgc
     1381 cgccgatggc catgaccatg aacgaggaac cgcgcttggt ctgcgcgccc aggtccttga
     1441 tgcccagggt gaaggtggtg gcgaacatgg ccgacatgaa gaagaacacg ccgaccagcg
     1501 caatggcgga caccttgtcg atgccggcgg cgacaatcag cgtgagcgcg acgttggcca
     1561 ggccatacgt cgtcaggatt tttcgcggat caaaacgcgt catcagcgcg gtgctgagga
     1621 agcggccgac caggtagagc agcgtggcga ccgacagcag gaaggcccct tcctgcgagc
     1681 tgaggccttt ccacgattca gtgaccaggt tgatgaagta cgcgccgatg cccacctgcg
     1741 cgccgacata cagcgcctgg gtgatcacgc caaagatgaa atggctgcgc gtggacagcg
     1801 gccgcgaggc gcccgtgtcc gtgatcgtcg ccgcctggat ctccggcagc tgcgcgcgcg
     1861 cgatgaagac ggcatagagc aggatcagcg cgccgatcac gccgtaggtc agctgtaccg
     1921 agtgcaattc ctcacccgcc gccgcggtgc cgccgaagaa caccgcgccg ccgatcatgg
     1981 gtccgagaaa tccgccgagg ccggtgaacg actgcgccag gttcaggcgc cgctccgcgt
     2041 gctccggcgc gccgagtacg ttcacgtagg tgtcggcgga ggtttcgaga aagcacaaac
     2101 ctgtcgccag cacgaacatt gcgccgacga agaacgggaa actcatcgcg ctcgccgccg
     2161 ggatgaacag gaaggcgcca ccggccgcca cggtaagacc ggtgaggatg cccaccttgt
     2221 agccgcgcgc gtgcatccac attcctgcgg ggatgctcat ggtgagatac gcgccgaaat
     2281 aggcgatctg cagccaggcc gactgcgcct tgcccacatg caggatttcc tggaagtgct
     2341 tgttcagcac gtcgagcagg ccgtaggcca ggccccagat gaaaaaggtg acgatggtgt
     2401 agcgcagcgg cgtgcgcagg tcgagcccgt gcgtgttgtc gttcatgctg ctatctccct
     2461 tcatgttctt gtaggagcgc gcttgcgcgc gatgctgcgg acgtgccatc gcgcgcaagg
     2521 cgcgctcctg catctgcatt cagatccggt aaatcgtctc ggccgtgccc gcaaacaacg
     2581 cgcgccgctc ggcatcggaa aaatccgcga cgatgctgct gtacgcctcg aaatgctggc
     2641 cgaaatttcc gtacagcttg tccaccggga agttgctggc gaacatcgcg cgctgcggcc
     2701 cgaacaggtc gatggtatcg agcacgaagg ggcggatgct gtcggtggtc cacgtgcggt
     2761 cgatcatcgc cagcccggaa atcttcaccg ccacgttggg ctgcttggcc agcagcgcca
     2821 tgccgcgttt c
//
LOCUS       DASZZC010000615         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_624083,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000615 DASZZC010000000
VERSION     DASZZC010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624083"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VHE37_13105"
     CDS             complement(<1..293)
                     /locus_tag="VHE37_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36519.1"
                     /translation="MNVISARWISGLMLVSAAVPVWACSSCGCTLGTEWADAGFSAAP
                     GLRVDLRYDLINQSRLRHGTHGASADDVAAALADGSAEETQVSTFTRFYTLGL"
     gene            complement(368..727)
                     /locus_tag="VHE37_13110"
     CDS             complement(368..727)
                     /locus_tag="VHE37_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36520.1"
                     /translation="MRTPRSIALHLLLVAALLLRALIPAGLMPGSGGGAPALVICTAD
                     GARFSLAHDGHHPAQQAHCPFQLALGMAGVAVAALALPLLRATELPAASPQGAAFPNP
                     VHHFYARGPPARSWLSF"
     gene            complement(801..2810)
                     /locus_tag="VHE37_13115"
     CDS             complement(801..2810)
                     /locus_tag="VHE37_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVT36521.1"
                     /translation="MKLKSGLIGLGAAALAAGCGMSLPGAVDKNATDEGMGVPVAAKA
                     LDSCDSSYADDTKALRPVDIDPAITYGAPARLILSFHSHAQSKSAMALLSSLTAVAGN
                     ELGLKLVAERNLFGFRVLPMLWIGVPAASEQLVQALAARLQPLGLVSIYADKPLRWFN
                     HESAEYTGVLKAHGDFNITGKGVGIAIVDSGIDQLQGDFSNVAINVKIVGPGNLTDGT
                     PAPQGYTYLDMTGLNSDTSSGHGTHVAGSAAGQGTMSNGYLAGMAPGATLIGVSTGEA
                     IAVLFSLEAYDYLLQPSVRQTYNLRVINNSYGDSAAVGFVPNDPTNIATKTAHDLGVW
                     SLFAAGNDGAVTDDGSKSDMTSYAASPCVMSVAAGSAAQNITDVNPNDYSPAFVGTFD
                     PSTFPTPPLPPQGTLTDFSSRGKAGDVYDHPDIVAPGYFIASAYNNTNGTIYYIGADA
                     TPYVDSSNPDWSPAWAASYFRISGTSQATPMIAGILALLLEANPVVDWDAAQDALYST
                     AHPVNDTAGAATAPWQTGAGFVDAYAAVQTVLATAGGRYDLVTDKIADLSGSVGASVS
                     APEVGSLQSSSAQSQFDLPAGADYTNLHVDISWTLPADDMDIVVLGPDGSTVGSSANS
                     PPSFESVDVPDPKPGTYTVIVNGYLNGPENYTGTASATHKVPHAG"
     gene            complement(2885..3895)
                     /gene="mtnA"
                     /locus_tag="VHE37_13120"
     CDS             complement(2885..3895)
                     /gene="mtnA"
                     /locus_tag="VHE37_13120"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HVT36522.1"
                     /translation="MTQTVQPILWANNHLRLLDQRVLPRATVYVECRNAAEVAQAIHG
                     MAVRGAPAIGIAAAYGLVLTAQRGDDFEQALLTLAASRPTAVNLRWALQRMKECWMRT
                     HDAAALLAEAQAIDREDLEQNRRMGRLGAALLPKHAVVITHCNTGALATGGHGTALGV
                     IRTAHEQGHLKRVYNTETRPWLQGARLTAWELQQEKIPSRLIADGAAAHLMSKEKIDW
                     VIVGADRIAANGDTANKIGTYMLAVVARRHGVKFMVVAPSGTFDLQCPDGAHIPIEER
                     HASELTMFRDHGIAPENYDAYNPVFDVTPAELIDAIVCERGVIEQPTGERVRACLGVS
                     SH"
     gene            3949..>5212
                     /locus_tag="VHE37_13125"
     CDS             3949..>5212
                     /locus_tag="VHE37_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HVT36523.1"
                     /translation="MQTIDLLVTPRWLVPIEPAGTVLEQHALAVDGGRILAVLPVAEA
                     RTRYTPRETLALPHHAVMPGLVNTHTHAAMTLLRGIADDLPLMDWLQHHIWPAEGAHV
                     GRAFCEDGARLAFAEMIAGGTTCCNDMYFFPDASAAVAAEAGLRVCVGLIVIDFPTAW
                     ASGPDEYFSKGLAVHDALKSQPLVKTVFAPHAPYTVSDAPMRKVRAYADELGIGIHCH
                     VHETAFEVADAQARTGGRPWQRLKELELLGPDFIAVHMTQLTDDEIAEAAQFGVHVAH
                     CPESNLKLASGFCPVHRLQQAGVNVALGTDGAASNNDLDLFGEMRSAALLAKAVSGDA
                     RALGAAQALHAATLGGARALGWDAEIGSLTAGKSADFIAIDFSATHMQPVYSVISQLV
                     YAAGRGDVTDVYVAGRALMRARELTTLDR"
BASE COUNT          857 a         1751 c         1762 g          842 t
ORIGIN      
        1 tccaagccca acgtgtaaaa gcgcgtgaag gtgctgacct gcgtttcctc cgcgctgccg
       61 tcggccagcg ccgcggcgac atcgtcggcg ctggcgccgt gcgtgccgtg gcgcaggcgg
      121 ctctggttga tcaggtcgta gcgcaggtcg acgcgcaggc cgggagccgc gctgaatccg
      181 gcatcggccc attcggtgcc cagcgtgcag ccgcaactcg aacaggccca caccggaacc
      241 gcggcactca caagcatgag gcccgatatc catcgggcag aaatgacgtt catcgaaaga
      301 tcctttgttt gttgctccgc atgcggagca ggaggcgcga aatgcgccga agttcaggca
      361 aacaacttca gaacgacaac caggatcggg cgggcggacc gcgtgcgtag aagtgatgaa
      421 ccggattggg aaaggccgcc ccttggggcg atgcggccgg aagttcggtg gctcgcaaca
      481 gcggcaaagc cagcgcagcg acagcaacgc cagccatgcc cagcgccagc tggaacgggc
      541 aatgcgcctg ctgcgccggg tgatggccat catgcgccag cgagaacctg gcgccgtcgg
      601 cggtgcagat caccagcgcc ggcgcaccac cgccgctgcc cggcatcagc ccggcaggga
      661 tcagcgcgcg cagcagcagc gccgcgacga gcagcaggtg cagcgcgatg gaacgtggcg
      721 tgcgcatggg cggcggattc tagcgcacgc aaaacgaacc agcccggacg cagccgggcc
      781 tgttggtcac gcggtgccgc tcatccggcg tgcggcacct tgtgcgttgc gctcgccgtg
      841 ccggtgtagt tctcaggccc gttgaggtac ccgttgacga tcacggtgta ggtgcccggc
      901 ttcgggtcgg gcacatccac gctctcgaag ctcggcgggc tgttggcgga gctgccgacg
      961 gtcgagccgt ccggaccaag caccacgatg tccatgtcgt cggccggaag tgtccacgaa
     1021 atgtcgacat gcaggttggt gtagtcggca cccgccggca ggtcgaactg gctctgcgcc
     1081 gacgaagact gcaacgaacc cacttccggc gccgacaccg acgcgccgac gctgccggaa
     1141 agatccgcga tcttgtccgt caccaggtcg tagcggccgc ccgcggtggc cagcacggtc
     1201 tgcaccgccg cgtaggcatc gacaaaaccg gcgccggtct gccacggcgc agtcgccgct
     1261 ccggcagtgt cgttcaccgg gtgcgccgtg ctgtacaagg catcttgcgc cgcatcccaa
     1321 tccaccacag ggttggcttc cagcagcagc gcgaggatgc cggcgatcat cggcgtagcc
     1381 tgcgaggtgc cggagatgcg gaagtacgac gccgcccacg ccggcgacca gtccgggttg
     1441 ctcgaatcca cgtacggcgt ggcgtcggcc ccgatgtagt agatggtgcc gttggtgttg
     1501 ttgtaggccg aggcgatgaa atagcccggc gcgacgatat ccggatgatc gtaaacgtcg
     1561 cctgccttgc cgcgcgagga gaagtcggtg agcgtgccct gcggcggcag cggcggcgtc
     1621 ggaaaggtcg acgggtcgaa cgtgccgaca aaggccggcg aatagtcgtt cgggttgacg
     1681 tcggtgatgt tctgcgccgc gctgcccgca gccacgctca tcacgcaggg cgaggccgcg
     1741 tacgaggtca tgtcgctctt gctgccgtcg tcggtgaccg cgccatcatt gccggcagcg
     1801 aacagcgacc acacgcccag gtcatgtgcg gtcttcgttg cgatgttggt cggatcgttg
     1861 ggcacgaagc ccaccgccgc gctgtcgccg taggaattgt tgatcacgcg caggttgtag
     1921 gtctggcgca ccgagggctg cagcaggtag tcgtaggcct ccaggctgaa cagcacggcg
     1981 atggcctccc cggtggacac gccgatcagc gtggcgcccg gagccatgcc ggccaggtaa
     2041 ccgtttgaca tggtgccctg ccccgccgcc gagcctgcca catgcgtgcc gtggcccgac
     2101 gaggtgtcgg aattgagccc ggtcatgtcc aggtaggtgt aaccctgcgg cgccggtgtg
     2161 ccgtcggtaa ggttgccagg gccgacgatc ttcacgttga tcgcgacgtt ggagaaatcg
     2221 ccctgcagct ggtcgatccc ggaatcgaca atggcgatgc ccacgccctt gccagtaatg
     2281 ttgaaatcgc cgtgcgcctt gagcacgccg gtgtattccg ccgattcgtg gttgaaccag
     2341 cgcagcggct tgtcggcgta gatcgacacc agccccagcg gctgcagcct ggcggccagc
     2401 gcttgcacca gctgctcgct cgccgccgga acgccgatcc acagcatcgg cagcacgcgg
     2461 aaaccgaaca ggttccgctc cgccaccagc tttaggccca gctcgttgcc ggcaaccgcg
     2521 gtgagcgatg acagcagcgc catggcgctc ttgctctgcg cgtgcgaatg gaaagacagg
     2581 atcagccgcg ccggcgcgcc gtaggtgatg gccggatcga tgtcgaccgg gcgcagcgcc
     2641 ttggtgtcat cggcatagga gctgtcgcag gaatcgagcg ccttcgccgc caccggcacg
     2701 cccatgccct catcggtcgc attcttgtcg accgcaccag gcaggctcat gccgcaaccc
     2761 gccgccagcg cagccgcgcc cagcccgatc aaaccggatt tcaacttcat ggaatttccc
     2821 cgaactcgtt gctcaccgcg cgccgtggtg atgtaaacga attgtaaaca gccgggcggc
     2881 ccgcttaatg agaggaaacc cccaggcagg ctctcacccg ttcgcctgtg ggctgttcga
     2941 tcacgccgcg ctcgcagacg atggcgtcga tcagctccgc cggcgtgacg tcgaaaaccg
     3001 ggttgtaggc gtcgtagttt tccggcgcga tgccatgatc gcggaacatg gtgagctcac
     3061 tcgcgtgccg ctcctcgatc gggatatgcg cgccgtcggg gcattgcagg tcgaaggtgc
     3121 cgctgggcgc caccaccatg aacttcacgc cgtggcgccg cgccaccacc gccagcatgt
     3181 aggtgccgat cttgttggcg gtatcgccgt tggccgcgat gcgatcggcg ccgacgatga
     3241 cccagtcgat cttctccttc gacatcaggt gcgcggccgc gccgtcggcg atcagccgcg
     3301 acggaatttt ttcctgctgc agttcccacg cagtgagccg cgcgccctgc agccagggcc
     3361 gcgtctcggt gttgtacacg cgcttcaggt gaccctgctc gtgcgcagtg cggatcacgc
     3421 ccagcgccgt gccgtgaccg ccggtggcca gcgcgccggt attgcaatgg gtgatgacca
     3481 ccgcatgctt cggcagcagc gcggcgccga gcctgcccat gcgccggttc tgctccaggt
     3541 cttcgcggtc gatggcctgc gcttccgcca gcagcgcagc ggcatcgtgc gtgcgcatcc
     3601 agcattcctt catgcgctgc aaggcccagc gcaggttcac cgcagtgggg cgcgacgccg
     3661 ccagcgtgag caacgcctgc tcgaagtcat cgccgcgctg cgcggtgagc accaggccgt
     3721 atgccgcggc gatgccgatg gccggcgcgc cgcgcacggc catgccgtgg atggcctgcg
     3781 ccacttcagc ggcgttgcgg cactctacat acaccgtggc gcggggcagc acgcgctggt
     3841 cgagcagtcg caggtggttg ttcgcccaca ggatgggctg cacggtctgg gtcatggcgg
     3901 gatttgagta gaattgctcg ccgcaattct actgacgtgc cctctcccat gcagaccatc
     3961 gacctgttgg tgacgccgcg ctggctggtg cccatcgagc cggccggcac ggtgctggag
     4021 cagcacgcac tggcggtcga cggcggccgc atcctggccg tgctgccggt cgcggaagcg
     4081 cggacgagat acaccccgcg cgaaacgctc gcgctgccgc atcacgccgt gatgccgggc
     4141 ctggtcaata cccacacgca cgcggcgatg accctgctgc gcggcatagc cgacgacctg
     4201 ccgctgatgg actggctgca gcaccacatc tggccggcgg aaggcgcgca cgtgggccgc
     4261 gccttctgcg aagacggcgc gcgcctcgcc ttcgccgaaa tgattgccgg cggcaccacc
     4321 tgctgcaacg acatgtactt cttccccgac gccagcgccg ccgtcgcggc cgaggccggc
     4381 ttgcgcgtct gcgtggggct gatcgtcatc gacttcccga ctgcgtgggc cagcgggccc
     4441 gacgaatatt tcagcaaggg cctggcggtg cacgacgcgc tcaagtcgca accgctggtc
     4501 aagaccgtct tcgcgccgca cgcgccgtac acggtgtcgg acgcgcccat gcgcaaggtg
     4561 cgcgcctatg ccgacgaact cggcatcggc atccattgcc acgtgcacga aacggcgttc
     4621 gaagtggcgg atgcgcaggc caggaccggc gggcgcccgt ggcagcggtt gaaggaactg
     4681 gaactgctcg gcccggattt catcgccgtg cacatgacgc agctgaccga cgacgagatc
     4741 gccgaagccg cgcagttcgg tgtgcacgtg gcgcattgcc cggagtcgaa cctcaagctc
     4801 gcctccggct tctgcccggt gcacaggctg cagcaggccg gcgtcaacgt cgcgctgggc
     4861 accgacggcg ccgccagcaa caacgacctc gacctgttcg gcgagatgcg cagcgctgcg
     4921 ctgctggcca aggcggtcag cggcgatgca cgcgcgctgg gcgcggcgca ggcgctgcac
     4981 gcagccacgc tcggcggcgc gcgcgcgctg ggctgggatg cggaaatcgg atctctgacg
     5041 gccggcaaat cagcggattt cattgcgatt gatttctcgg ccacgcacat gcagccggtc
     5101 tatagcgtca tttcgcagct ggtctacgcg gccggtcgcg gcgacgtcac cgacgtgtat
     5161 gtcgccgggc gcgcgctgat gcgcgcgcgc gaactgacca cgctggatcg cg
//
LOCUS       DASZZC010000616         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_499367,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000616 DASZZC010000000
VERSION     DASZZC010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499367"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VHE37_13130"
     CDS             complement(<1..1492)
                     /locus_tag="VHE37_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HVT36524.1"
                     /translation="MGKLIRIGCGAGFWGDSAEGPKQLVESGQIDYLVLDYLAEITMS
                     LLAKARAKDPNAGYATDFPDVIARLAPQIKARGIKVVTNAGGVNPQACREAIATKLKA
                     QGIELKIGIVTGDDLLARSEEFRAQDIKEMFNGSAFPAKPWSSNAYLGAFPIAAALDA
                     GADIVITGRCVDSAIVLGPLVHEFKWAHDDYDRLSAGSLAGHIVECGVQATGGIVTDW
                     KEVAADWDRMGFPIAEVSADGGCVITKPANTGGRVAPETVAEQIIYEIGDPAEYLLPD
                     VTCDWSAVQLTQIAPDRVQLRGARGRAPSASYKVSTTFQDGFRATGTMMMGGPDAAGK
                     ARAVADAILKRTRRMMSERGFADYRRTDVEVLGAEANWGANARAGATREVILKLALHH
                     DNKDALEIFSREFIPAATAMAQGITGFSGGRPGVTPVVRLFSWLVPKASVEAKADVDG
                     LAVEVAAAPAGARPAHDAGSESRARPAPATAAIPGATKIVPLVQLAY"
     gene            complement(1586..2425)
                     /locus_tag="VHE37_13135"
     CDS             complement(1586..2425)
                     /locus_tag="VHE37_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVT36525.1"
                     /translation="MDVVLLERKPCAAVAGSSVVPAAVSGGNERWVQWITINRADKRN
                     AINEEVIAGIARGVAQASSDLTCRAVVLTGAGDKAFCAGADLAKNVQGGAFAVDFSQP
                     RHYIVDLFKLMQECRLPIVARVNGHVMAGGFGLLCSCDLAVAADDIRIGTPESKIGMT
                     PMMILPSMIRVLPPRKLQEMCITGEQFSAQDALEWGVVNYIAPRAEFDAKLDWLLSRI
                     TDKSPTAVRLGKQAYNAMRDMSLRDSLEYAQAMVPVMASTQDAKEGMAAFQEKRAPNW
                     TGR"
     gene            2735..>3166
                     /locus_tag="VHE37_13140"
     CDS             2735..>3166
                     /locus_tag="VHE37_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36526.1"
                     /translation="MTLAGKVAMITGAAGSLGKSVADAFISAGAKLILVDLDEGAMRR
                     VYGDSSAHVYAAANLTDAQASRAALTAAIGKAGRVDALCNIAGGFRMGPAVHETPEDL
                     WKLMMDMNAGTLLHACAAVVPAMLAQGGGRIVNVGAGAGQKG"
BASE COUNT          511 a         1049 c         1054 g          552 t
ORIGIN      
        1 cataggcgag ctgcaccagc ggcacgatct tcgtcgcgcc tgggatcgcg gctgttgcag
       61 gagcgggcct tgcccgcgat tcggagcctg catcgtgcgc agggcgcgct cctgcaggtg
      121 cagccgcgac ttcgaccgct agcccatcga catcggcctt ggcctcgacg ctcgccttgg
      181 gcaccagcca cgaaaacagc cgcaccaccg gcgtcacgcc cggccggccg ccggaaaacc
      241 cggtgatgcc ctgcgccatg gcagtcgctg cggggatgaa ttcgcgcgag aagatttcca
      301 gcgcgtcctt gttgtcgtga tgcaaagcaa gcttgaggat cacctcgcgc gtcgcaccag
      361 cgcgtgcgtt ggcgccccag ttggcctcgg cgccgagcac ttcgacatca gtgcggcgat
      421 agtcggcgaa accgcgctcg ctcatcatgc gccgcgtgcg cttcaggatg gcatcggcca
      481 ccgcgcgcgc tttgcccgcc gcgtccggcc cgcccatcat catggtgccg gtggcacgga
      541 aaccatcctg aaacgtcgtg ctgaccttgt agctggcgct gggcgcgcgt ccgcgcgcac
      601 cacgcagctg cacgcggtcc ggcgcgatct gtgtgagctg caccgcactc cagtcgcagg
      661 tcacgtcggg cagcaggtat tcggccgggt cgccgatctc gtagatgatc tgctcggcca
      721 ccgtttccgg cgccacgcgg ccgccggtgt tcgccggctt ggtgatgacg cagccgccat
      781 cggccgacac ttccgcgatg gggaagccca tgcggtccca gtcggcggcg acctccttcc
      841 agtcggtgac gatgccgccg gtcgcctgca cgccgcactc gacgatgtgg ccggccaggc
      901 tgccggcgga gagccggtcg taatcgtcgt gcgcccactt gaactcgtgc accagcgggc
      961 cgagcacgat ggcgctgtcg acgcagcggc cggtgatgac gatgtcggcg ccggcatcaa
     1021 gcgccgcggc gatcggaaac gcgccgagat aggcgttgct gctccacggt ttcgcgggga
     1081 aagctgagcc gttgaacatc tccttgatgt cctgcgcgcg gaattcctcc gagcgcgcca
     1141 gcaggtcgtc gccggtgacg atgccgatct tcagttcgat gccctgcgcc ttcaatttgg
     1201 tggcgatggc ctcgcggcag gcctgcggat tgacgccgcc ggcgttggtc accaccttga
     1261 tgccacgcgc cttgatctgc ggcgccaggc gcgcgatgac gtcggggaaa tcagtggcgt
     1321 aacccgcgtt cggatctttc gcgcgcgcct tggccagcag cgacatggtg atctcggcca
     1381 ggtagtcgag caccaggtaa tcgatctggc cggactcgac cagctgcttc gggccctcgg
     1441 cggaatcgcc ccagaagccg gcgccgcagc cgattctgat gagcttgccc atcagtatcg
     1501 tccccgcgca ggcggggacc cggggtttgt ttgagaggaa aactggattt ccgccttagc
     1561 gggaatgacg gcaaaagcag ccatctcagc gccccgtcca gttgggagca cgcttttcct
     1621 ggaacgccgc catgccttcc ttcgcgtcct gcgtggaggc catgaccggc accatggcct
     1681 gcgcgtactc cagcgaatcg cgcagcgaca tgtcgcgcat ggcgttgtag gcctgcttgc
     1741 ccaggcgcac ggcggtgggc gacttgtcgg tgatgcgcga cagcagccag tccagcttgg
     1801 cgtcgaactc ggcgcgcggc gcgatgtaat tcaccacgcc ccactccagc gcgtcctgcg
     1861 cgctgaactg ctcgccggtg atgcacatct cctgcagctt gcgcggcggc agcacgcgga
     1921 tcatcgacgg caggatcatc atcggcgtca tgccgatctt gctctccggc gtgccgatgc
     1981 ggatgtcgtc ggcggcgacg gcgagatcgc acgagcacaa caggccgaag ccgccggcca
     2041 tgacgtggcc gttgacgcgc gccacgatcg gcagccggca ttcctgcatc agcttgaaca
     2101 ggtcgacgat gtaatggcgc ggctgcgaga agtcgacggc gaaggcgccg ccctgcacgt
     2161 tcttggccag gtccgcaccg gcgcagaaag ccttgtcgcc ggcgccggtg agcacgacgg
     2221 cgcggcaggt gagatcgctg ctggcctggg ccacgccgcg cgcgatgccg gcgatcactt
     2281 cctcgttgat cgcgttgcgc ttgtccgcac ggttgatggt gatccattgc acccatcgct
     2341 cattgccgcc ggagacggcg gcgggtacca cgctgctacc tgcaacagca gcgcatggct
     2401 tgcgctcgag caagacgacg tccatggaaa ctttctccga atgaggccgc gcaagactag
     2461 acggcgcttg gtgagtgatc ctcacccgaa cgggggagcc gcctcaatgg cgtgcgcaga
     2521 gccggattgg cgcttcagga gccataccgc acctccggtt ttgatgttat taattattta
     2581 tcgtttatat aattatatga gcttttataa catgataaac ctcggtgcgg ttggagcttt
     2641 ttccggccca tgcaagccag ttcacccatg cggaggattg catggcgcgc acgcgtgcta
     2701 gcgtgcacgc aggcgaactc acgaggagca tgcgatgact cttgcgggca aggtggcgat
     2761 gatcaccggc gcggccggca gcctgggcaa gtcggtggcg gatgccttca tcagcgcggg
     2821 cgcgaagctg atcctggtcg atctcgacga aggcgcgatg cgccgcgtct acggagattc
     2881 ctccgcgcat gtctacgccg ccgccaatct caccgatgcg caagcctcgc gcgcggcgct
     2941 caccgccgcg atcggcaagg ccggccgcgt cgacgcgctg tgcaacatcg ccggcggctt
     3001 ccgcatgggc ccggccgtgc atgaaacgcc ggaagacttg tggaagctga tgatggacat
     3061 gaacgccggc acgctgctgc acgcctgcgc cgcggtggtg ccggcgatgc tcgcgcaggg
     3121 cggcggcagg atcgtcaacg tcggcgccgg cgccgggcag aagggc
//
LOCUS       DASZZC010000617         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_244460,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000617 DASZZC010000000
VERSION     DASZZC010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244460"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VHE37_13145"
     CDS             complement(<1..608)
                     /locus_tag="VHE37_13145"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HVT36527.1"
                     /translation="MLVLAALAGVIGLSWWWLVRAARDMYGAMDGPAAWMMDASWDAR
                     YLALICAMWAVMMAAMMLPSAIPAVLMYQRVMGGPQWSSTAARTVLFAAGYLLAWSAF
                     SVIATVLQWQLARAALVSPMMQSTRPLLDGVLLMGAGLYQWLPLKRSCLALCRAPADF
                     LAHRFRPGAGGALIMGLQHGMICIGCCWALMLLLFVGGVMNLA"
     gene            complement(635..1270)
                     /locus_tag="VHE37_13150"
     CDS             complement(635..1270)
                     /locus_tag="VHE37_13150"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HVT36528.1"
                     /translation="MSKVEWSFKGVELANCNCAYGCPCQFNALPTHGNCRAMTAFRVE
                     SGHFGEVKLDGLDFVIMGAWPGAIHQGNGTWMSIIDERADEKQRKALEAITHGEHTDP
                     GGSIFQIFNSMVTKKLPTLYRPIRFEVDRAARSGKVSVPGVLDTSAEPIRNPVTGAVH
                     KVSVTLPEGFEYTQADFVSGHTRTHGDIVLQFTGTHAHIAPIHFSAHGVVR"
     gene            complement(1419..>2078)
                     /locus_tag="VHE37_13155"
     CDS             complement(1419..>2078)
                     /locus_tag="VHE37_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36529.1"
                     /translation="NAWNNDAGSTVHYVYAGTTTASGGLNQSDGVNTVLFNDPDNDIA
                     GAFDCFNGGVLAYSSYRTRGTRSFNGGTYAVIVEVDTVVQDGASCVLANHGNRDAAEL
                     LTHELGHTLGLGHSCGDSGLVACVLGSLLDLATMRPYIHADGRGAVLGDDDRAGIDFL
                     YPSGVAAPATPAGSSGSGAAAASNSGGGSMSGFTLYVLAIGALLGLLRKKKQMEPHRT
                     A"
BASE COUNT          348 a          698 c          686 g          346 t
ORIGIN      
        1 gccaggttca tcacgccgcc gacgaacagc aggagcatca gcgcccagca gcaaccgatg
       61 cagatcatgc cgtgctgcag gcccatgatc agcgcgccgc cggcgcccgg gcggaaccgg
      121 tgcgcgagga aatccgccgg cgcgcggcac agcgcgagac agctgcgctt gagcggcagc
      181 cactggtaga ggccagcacc catcagcagc acgccatcaa gcagcggccg cgtgctctgc
      241 atcatcggcg acaccagcgc cgcgcgcgcc agctgccatt gcagcaccgt ggcgatgacc
      301 gaaaaggcgc tccaggccag caggtaaccg gcggcgaaca gcaccgtgcg cgcggcggtc
      361 gacgaccatt gcggcccgcc catcacccgc tggtacatga gcacggcggg aatggcgctt
      421 ggcagcatca tcgcggccat catcacggcc cacatggcgc agatcagcgc gaggtagcgg
      481 gcatcccagc tggcgtccat catccacgct gcgggcccat ccatggcgcc atacatgtcg
      541 cgcgcggcgc gcaccagcca ccaccaggag agtccgatga cgccggccag cgcggccagc
      601 accagcacgc gtgcgcgcgc cggtgtgccc gccgtcagcg caccactccg tgcgcggaaa
      661 aatggatcgg cgcgatatgc gcgtgcgtgc cggtgaactg cagcacgatg tcaccgtgcg
      721 tgcgcgtgtg gccgctgacg aaatccgcct gcgtgtactc gaagccttcc ggcagcgtca
      781 cgctcacctt gtgcacggcg ccggtcaccg ggttgcggat cggctcggcg ctggtgtcga
      841 gcacgcccgg cacgctgacc ttgccgctgc gcgcggcgcg gtccacctcg aagcggatcg
      901 gccgatacag cgtcggcagt tttttcgtca ccatggaatt gaagatctgg aagatgctgc
      961 cgcccggatc ggtgtgctcg ccgtgggtga tcgcttccag cgccttgcgc tgcttttcat
     1021 cggcccgctc gtcgatgatg ctcatccagg tgccgttgcc ttggtgaatc gccccaggcc
     1081 acgcgcccat gatgacgaaa tccaggccgt cgagtttcac ctcgccgaag tgcccgctct
     1141 ccacgcggaa tgcggtcatg gcgcggcagt tgccgtgcgt gggcagcgcg ttgaactggc
     1201 acgggcagcc gtaggcgcag ttgcagttgg ccagctccac tcccttgaag ctccattcga
     1261 ccttgctcat ggcgagctcc tggtccggtg cggggtgggc agcagcatac atccgccgat
     1321 tgcctcaacg cgccaggaaa ggcctgaccg caccgctgcc ttgcctgtta tttattatta
     1381 tacgttttta aatttataca tctttttata acatcaggtc aggcggtgcg gtgaggctcc
     1441 atctgcttct tttttcgtag caggccgagc aaggcgccga tggcgagcac gtaaagcgtg
     1501 aagccgctca tgctgccgcc accgctgttg ctggcggccg cagcgccgct gccgctggaa
     1561 cccgcgggcg tcgccggggc cgccacgccg gatggataaa ggaaatcgat gccggcgcgg
     1621 tcatcgtcgc ccagcaccgc gccgcgtcca tcggcatgga tgtacgggcg catggtcgcc
     1681 aggtcgagca gtgagccgag cacgcaggcc accagcccgc tgtcgccgca ggaatggccg
     1741 aggccgagcg tgtggccgag ctcgtgcgtg agcagttccg cggcgtcgcg gttgccatgg
     1801 ttggccagca cgcaggaagc accgtcctgc accaccgtgt ccacctcgac gatgaccgcg
     1861 taggtgccgc cgttgaaact gcgcgtgccg cgcgtgcgat agctggagta ggccagcacg
     1921 ccgccgttga aacagtcgaa ggcgccggcg atgtcgttgt cgggatcgtt gaacagcacg
     1981 gtgttgacgc catcggactg gttgagcccg ccgctggccg tggtggtgcc ggcatagacg
     2041 tagtgcaccg tgctgccggc gtcgttgttc cacgcgtt
//
LOCUS       DASZZC010000618         4475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_374297,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000618 DASZZC010000000
VERSION     DASZZC010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374297"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VHE37_13160"
     CDS             complement(<1..1473)
                     /locus_tag="VHE37_13160"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HVT36530.1"
                     /translation="MKSSPTRLTILLAGLWIGAAQAAPECNVATLQKLAPAGVQVTSA
                     QRRQNGQSPYCEVEGTMPMTGNSLRYRMGLPEAWNGKFLFEATGGGAGVLVGMGPGAQ
                     RGYAVATTDTGHEGSVGDFSFWLDRSKRAGWIHEGVHTATVGAKAMVVAYYGRTPQQS
                     FLRGCSNGGRAGLMEAQRYPEDYDGILSAAPAADPGAITINWIRDAQILHRTPLDRDD
                     WNLVGRTVRESCDARDGLHDGMIQDSRRCVLPREQLACKAGRTSGCLGDAKLAALDQL
                     WNKPQIAGDVLLFDEGHGYEDQMPAQAFYAGASPWTLLRLLATSGGGSGNEFSMRATE
                     PPPPLRTLLPWLVMGAPSAMDQFVESFGAGVLMPDRRIDVRQFDVAKDGPELARQIRA
                     IISDTDRPDLAAYLKRGSKLLLWQGMDDVTLPPGGTFHYFDLAREHALRDGISVEAFD
                     QSVRLYTAPTIGHCAGGDGPNEGELFGALDAWVTQAKTPEA"
     gene            complement(1554..2333)
                     /locus_tag="VHE37_13165"
     CDS             complement(1554..2333)
                     /locus_tag="VHE37_13165"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVT36531.1"
                     /translation="MDLPLLWHIPFSHYNEKARWALDYKRIAHRRRSIGVDYLYRAWR
                     ATGRGTLPILFLDGRAIGDSTAIIAALEQRQAEPPLYPRDPAQRERALALEDYFDEVL
                     GPSLRAALVSPLFEQSADLGVRVLTTGMPAQTYRNMRPLIWLFPAFYRWRHKISAAQL
                     QRHRANVSAALDRISQELQPGGYLAGDNFCVADLTAAALLAPLLQPAELQYHLAMDMP
                     ESMRRYRDETLQHPAAQWALEIYRRHRGTSAEVRRQRAAPA"
     gene            complement(2377..2982)
                     /locus_tag="VHE37_13170"
     CDS             complement(2377..2982)
                     /locus_tag="VHE37_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVT36532.1"
                     /translation="MEPARLLAFCGSFFLIALSPGLCMTLAMSLGISIGVRRTLWMML
                     GEVAGIALVGASAMAGVAALLLGAPPVFVTFKIAGAAYLLWSAWRTWHAHAAAQAAPR
                     HITPRALCAQGFITAVSNPKAWAFNMALLPPFIDAHAALAPQMAQLLALMVLIEFSCL
                     LIYAQGGRALSELLLRRGQAQWLNRVAAALMVGVAGWLLLG"
     gene            complement(3050..>4475)
                     /locus_tag="VHE37_13175"
     CDS             complement(3050..>4475)
                     /locus_tag="VHE37_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36533.1"
                     /translation="RPRRSPSDLAYQFGDLKLAGNFDWAQLISDTGYVRKSSDTFFDA
                     SSRLPGNGQTPAQTAQIDYARSHTWSQELRLVSPDDSAHWQWVAGAAAFKQDVLFHEE
                     LPVSVALPLGLNQIEAALGPALNAIPGLAQQIDANGQALLALLETNAEIREYALFGDL
                     TRRLGDSWEVSLGGRLYRTTSQGTARQEGLLLLAINQQPTHLIEGTLHEQGFNPKASL
                     MWHAGRDLIAYVSASKGFRVGGVQPGYSASGPKAPDFFKSDTLWNYEAGLRTQWFDRQ
                     LRADITGFYVTWKNPQTFQLGSSGLSSYLDNAGGVKNHGIEAALQAVLPFGFLLNTSL
                     CWTDTRTSKPFDSQGGEVAPGAQWPFAPHWQTASSLAWMLPLGNWTLAPSVNYSTLSH
                     APTTLDPSAVQFVFGYSQLDLQLSVNNASLPWMPEFALTLNNVRDKRGLVNRFDGGLV
                     PGSGYTDVTYMRPRALTLHLAGHF"
BASE COUNT          671 a         1502 c         1618 g          684 t
ORIGIN      
        1 cgcctccggc gtcttcgcct gggtaaccca ggcgtcgagc gcgccgaaca gttcgccttc
       61 gttggggccg tcgccgccgg cgcagtggcc gatggtcggc gcggtgtaca ggcgcaccga
      121 ctggtcgaac gcctcgacgg aaatgccgtc gcgcaatgcg tgctcgcggg ccaggtcgaa
      181 ataatggaag gtgccgccgg gcggcagggt gacgtcatcc atgccctgcc acagcagcag
      241 cttcgagccg cgcttgagat aggccgcaag gtcgggacga tcagtgtcgc tgatgatggc
      301 gcggatctgc cgcgccagtt cggggccgtc cttggccacg tcgaattgcc gcacgtcgat
      361 gcggcggtcc ggcatcagca cgccggcgcc gaagctttcc acgaactggt ccatggccga
      421 gggcgcgccc atcaccagcc agggcagcag cgtgcgcagc ggaggcggcg gctcggtcgc
      481 gcgcatggag aattcgttgc cgctgccgcc gccggaggtg gccagcaggc gcagcagcgt
      541 ccacggcgac gcgccggcgt agaacgcctg tgccggcatc tggtcctcgt agccatggcc
      601 ctcgtcgaac agcagcacgt cgccggcgat ctgcggcttg ttccacaact gatcgagcgc
      661 ggccagcttg gcgtcgccga ggcatccgct ggtgcgcccc gctttgcacg ccagctgttc
      721 gcgcggcagc acgcagcggc ggctgtcctg gatcattccg tcgtgcagcc cgtcgcgcgc
      781 gtcgcagctt tcgcgcacgg tgcggccgac caggttccag tcgtcgcggt cgagcggcgt
      841 gcggtgcagg atctgcgcgt cacggatcca gttgatggtg atggcgccgg gatcggccgc
      901 cggcgcggcg gacaggatgc cgtcgtaatc ctccgggtag cgctgcgctt ccatcaggcc
      961 ggcgcggccg ccgttggaac agccgcgcag gaacgactgc tgcggagtgc ggccgtaata
     1021 cgccacgacc attgccttgg cgccgaccgt cgcggtgtgt acgccttcgt ggatccagcc
     1081 ggcgcgcttg ctgcggtcga gccagaagga gaaatcaccg accgaacctt catggccggt
     1141 gtcggtggtc gccaccgcat agccgcgttg cgcgccgggg cccatgccga ccagcacgcc
     1201 cgcgccgccg ccggtggcct cgaacagaaa cttgccgttc cacgcttccg gcaggcccat
     1261 gcgatagcgc aggctgttgc cggtcatcgg catggtgcct tcgacttcgc agtacggcga
     1321 ctggccgttc tgccggcgct gcgcactggt tacctgcact cccgcgggcg cgagcttctg
     1381 cagcgtggcc acgttgcact cgggcgcagc ctgcgcggcg ccaatccata aaccggccag
     1441 caggatggtc agcctcgtgg gactggattt catttctgtt ctcctcgacc cgggccaagg
     1501 tagcagccgg ccgcgcggct gcacctcccc aaaggagggg ctgcaggagt ggctcaggcc
     1561 ggcgctgcgc gctgacgccg cacttcggcc gacgtgccgc gatgccgccg gtaaatttcc
     1621 agtgcccatt gcgccgccgg gtgctgcagc gtctcgtcgc ggtagcggcg catgctctcc
     1681 ggcatgtcca tcgccaggtg gtattgcagt tcagccggct gcagcagcgg tgcgagcagc
     1741 gcggccgcgg tgagatcggc aacgcagaag ttgtcgccgg cgagataacc gccgggctgc
     1801 agctcctgcg aaatgcggtc gagcgccgcg ctcacgttgg cgcggtgccg ctgcagctgc
     1861 gcggcactga tcttgtggcg ccagcggtag aacgccggga acagccagat cagcgggcgc
     1921 atgttgcggt aggtctgtgc cggcatgccg gtggtgagca cgcgcacgcc caggtcggcg
     1981 ctctgctcga acaaaggact gaccagcgcg gcgcgcaggc tcggcccgag cacctcgtcg
     2041 aaataatcct ccagggccag cgcgcgctcg cgctgcgccg gatcgcgcgg atacagcggc
     2101 ggttcggcct ggcgctgctc cagtgcggcg atgatcgcgg tggagtcgcc gatcgccctg
     2161 ccgtcgagaa acaggatcgg cagcgtgccg cgaccggtgg cgcgccacgc gcggtagaga
     2221 taatcgacgc cgatgctgcg ccgccggtgc gcgatgcgct tgtagtccag cgcccagcgc
     2281 gccttctcgt tgtagtgcga aaacgggatg tgccagagca gcggcaggtc catgcggcag
     2341 ttcccggcgg cggtgtgcgc gagtgtagcg aagccctcag ccgagcagca gccagccggc
     2401 cacgccgacc atcagcgcgg cggcgacgcg attgagccac tgcgcctggc cgcggcgcag
     2461 cagcagctcg ctgagcgcgc ggccgccctg cgcgtagatc agcaggcagc tgaattcgat
     2521 cagcaccatc agcgccagca gctgcgccat ctgcggtgcg agtgcggcat gtgcgtctat
     2581 gaacggcggc agcagcgcca tgttgaaggc ccaggccttg ggattggaca cggcggtgat
     2641 gaagccctgc gcgcacagcg ctcgcggagt gatgtgccgc ggtgctgcct gcgccgccgc
     2701 gtgcgcgtgc caggtgcgcc acgcgctcca cagcaggtag gcggcgccgg cgatcttgaa
     2761 ggtgacgaac accggcggcg cgcccagcag cagcgccgcc acgccggcca tggccgaggc
     2821 gccgaccagc gcgatgccgg cgacttcgcc gagcatcatc cacagggtgc ggcgcacgcc
     2881 gatgctgatg cccagcgaca tggccagcgt catgcacagg cccgggctga gcgcgatcag
     2941 gaagaagctg ccgcagaagg cgagcaggcg ggcgggctcc atgggcgcgg gcatcgggcg
     3001 gaaagcgggc gcacatcatc gcatgcgcac cgcgccaccc gtgccgcggt cagaaatgcc
     3061 cggccaggtg cagcgtcagc gcgcgcggcc gcatgtaggt gacgtcggtg tagccggagc
     3121 ccggcaccag gccgccgtcg aagcggttga ccagcccgcg cttgtcgcgc acgttgttca
     3181 gggtgagcgc gaactcgggc atccacggca gcgaggcgtt gttcaccgac agctgcaggt
     3241 cgagctggct gtagccgaac acgaactgca ccgcgctcgg gtcgagcgtg gtcggcgcgt
     3301 ggctcagcgt ggaatagttg acgctgggcg ccagcgtcca gttgcccagc ggcagcatcc
     3361 acgccagcga gctggcggtc tgccagtgcg gcgcgaacgg ccattgcgca cccggcgcca
     3421 cttcgccgcc ctgcgagtcg aacggcttgc tggtgcgcgt gtcggtccag cacagcgagg
     3481 tgttgagcag gaagccgaac ggcagcaccg cctgcagcgc ggcctcgatg ccgtggttct
     3541 tcacgccgcc ggcgttgtcc aggtacgacg acaggccgct ggagccgagc tggaaggtct
     3601 gcggattctt ccaggtgacg tagaagccgg tgatgtcggc gcgcagctgg cggtcgaacc
     3661 actgcgtgcg caggccggcc tcgtagttcc acagagtgtc cgacttgaag aagtccggcg
     3721 ccttgggccc gctggccgag tagccgggct gcacgccgcc gacgcgaaag cccttcgacg
     3781 ccgacacata ggcgatcagg tcgcggcccg cgtgccacat cagcgaggct ttgggattga
     3841 agccctgctc gtgcagcgtg ccctcgatca ggtgcgtggg ctgctggttg atcgccagca
     3901 gcagcaggcc ttcctggcgc gccgtgccct gcgaggtggt gcggtacagg cgcccgccca
     3961 ggctcacttc ccagctgtcg ccgaggcggc gggtgaggtc gccgaacagc gcgtattcgc
     4021 ggatctcggc gttggtctcc agcagcgcga gcagcgcctg gccgttggcg tcgatctgct
     4081 gcgccaggcc gggaatcgcg ttgagcgcgg gaccgagcgc ggcctcgatc tggttgagcc
     4141 ccagcggcag cgccacgctc accggcagct cttcgtggaa cagcacgtcc tgcttgaacg
     4201 cggcggcgcc ggccacccac tgccagtgcg cgctgtcgtc gggcgagacc aggcgcagtt
     4261 cctggctcca ggtgtgcgag cgcgcgtagt cgatctgcgc cgtttgcgcg ggtgtctggc
     4321 cgttgccggg caggcgcgac gaggcatcga aaaaggtgtc gctgctcttg cgcacgtagc
     4381 cggtgtcgga gatcagctgc gcccagtcga aatttccggc gagcttgaga tcgccgaact
     4441 ggtaggcgag gtcgctggga ctgcgccgcg gacgg
//
LOCUS       DASZZC010000619         7388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_956854,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000619 DASZZC010000000
VERSION     DASZZC010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956854"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VHE37_13180"
     CDS             <1..1127
                     /locus_tag="VHE37_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVT36534.1"
                     /translation="EFAPEAHEGDPAVFQARAAALPALPPSPVQKADIVVWLNESTFD
                     PRNYHLPGARLPHLRMMDAAPTTRARDHLRVHTYGGKTWLSEFSLLTGLVPDDFGARR
                     NLVFNSVTPNTTSNLVRALKADGYKTVVLMPTFKRFYGAGRTYLGTGFDQVLTLRDFP
                     EYDNVPGDEWDIADSDRLAQAAQTIVERHRAGPDGAQPLLLYLLSIKEHAPYSKKTPI
                     DYALQRVPIKESLQHKLSDYLGKLVKLDNALLALQQALFRNGERPVVFAWFGDHQAYF
                     EEDAPPYTYDLPKPDYLTQFQLRTNFNAPATATGDYFDIAFLPSLLIDYAGARPDDYF
                     AGLSAMRRLCGGLMDECADKALVQSYKAWVFSPGLGLFAQ"
     gene            complement(1113..1370)
                     /locus_tag="VHE37_13185"
     CDS             complement(1113..1370)
                     /locus_tag="VHE37_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36535.1"
                     /translation="MEPDQVLVKTDKGREEIKTRTLALSQAARTLLIASDGQKTVRSM
                     TELFARMPGISGMLQELVDHGLIELRGGAAASSQRVSLLRK"
     gene            1549..2379
                     /locus_tag="VHE37_13190"
     CDS             1549..2379
                     /locus_tag="VHE37_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36536.1"
                     /translation="MPAHSPARTIVITGGSSGIGAEAARQLARQGHTLCLVARRREEL
                     EKVQAEIAAEGGKAHIYAADLADGAAADACCKTILAEHARVDVLVNNAGRSIRRPLLE
                     SLERAHDFERTMQINYFAAVRMTLGLLPRMLQQKDGHIINISSVVALMRTPRFAAYVG
                     SKSALDAFGHTLAMELHDAGIRVSTVNFPLVKTAMTAPTAAYNYVEQMDVRHAAGWIV
                     DAVRTRRVRRTTWDAVLGITAIGLAPGLALATLRRYYKRRVRLMQKRMQEAAQSQQSS
                     "
     gene            2445..3233
                     /locus_tag="VHE37_13195"
     CDS             2445..3233
                     /locus_tag="VHE37_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT36537.1"
                     /translation="MKFSGLMNSAVADGHALSVDVGEDWLQGRSVFGGLQAALMLAAM
                     RRLVPAALPLRTLQVTFIAPIGAGTLRAQATLLRTGKSAMHVQAVLADPAGATLCLAA
                     AVFGASRESQVRVTPQRPAIPGGTPIDFRYVQGLTPNFTRHYSMRWLLGNVPFSGTPG
                     ERAVIEVGVHDDGAASEGHLLGIADVIPPVALSRFRQPVFGSSLTWAIDFLLDRYDTQ
                     PLTGWRLDAELVGARDGYTSQDVHVWAPDGQLAALSRQCMVVFG"
     gene            complement(3240..3788)
                     /locus_tag="VHE37_13200"
     CDS             complement(3240..3788)
                     /locus_tag="VHE37_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVT36538.1"
                     /translation="MTSARIAPLDPPYTAEMQADFDKVMRGLPPLKLFRTVGRNPRVL
                     SRLIAGGLLDRGSISLRARELMILRTCARCGAEYEWGVHVAAYNTKAGFTPEQLHASV
                     HGDAAAPVWTREDAAVVKLADALHEKHDVNDALWGELKSVYADDQLIELVMLAGLYRA
                     VSYLINATRLEREAMAPRFPAA"
     gene            3914..4198
                     /locus_tag="VHE37_13205"
     CDS             3914..4198
                     /locus_tag="VHE37_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT36539.1"
                     /translation="MALLDLLGRRWNMRLLWELRGEPLNFRALQQACGGLSPSVLNTR
                     LAELRQAQLVDGGDAGYTLSRQGQALLKQFAPLNRWAEGWAAGLGKKRGK"
     gene            complement(4205..4855)
                     /locus_tag="VHE37_13210"
     CDS             complement(4205..4855)
                     /locus_tag="VHE37_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36540.1"
                     /translation="MINWTRRALYGLALAYAGHAAASEPGTRATRPVPAPSMVNPLAA
                     QWLAAGYPVTLTAQPDPLDPRAHRSGSDLALHPVAIGQDAPLLTATLVDELAQMKDGV
                     GLDLKVPDVTNLYLNLYTRSNPRSRGKRVQLAPDDSDDGSAEPLWSVGGAVNVVRELD
                     GGRRLEVAPQLTVNLGRAVGAPGRLQALLQRARWNNNDPFNGGTESVVQLSFRWRF"
     gene            5054..5941
                     /locus_tag="VHE37_13215"
     CDS             5054..5941
                     /locus_tag="VHE37_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVT36541.1"
                     /translation="MSIGIGEIYSLGCALAWAIAVILFKKSGESLPPLALNLFKNALS
                     LVMIGATIVLLRGPQLPQIPPMALGLILLSGLLGIAAGDTYYFQALNAIGAGRIAVAQ
                     TLYSPFVITLSMTFLGEHLRPLQFGGVALVLGGIVLVTYVRERNHPDAHRLRRGVLTA
                     VLAMFCTAVGIVMAKPMLAQHDFLWVVWLRIAGGFFGLCAVVLARGDAGEMRARCARV
                     RHWPQVIGGTVMGNYVSMMLWLGGYKYTKASIAAVLNELAAVFIVLFAVLFLHERASA
                     RQVAGVLLAVAGVVMVVAL"
     gene            complement(6056..>7388)
                     /locus_tag="VHE37_13220"
     CDS             complement(6056..>7388)
                     /locus_tag="VHE37_13220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVT36542.1"
                     /translation="AFSVRIAGKTEPWSTNFDIYRVPADGSKAPENLTASNPAWDTSP
                     LFSADGNTLYYRAMKRPGFEADRFWLMEKNLKTGAVREIAPRWDRSPDALALSPDGRS
                     IYCYADDIGQRPLFAVDIATGNVTRISGQGTVSGFTLAADGIVYAQNSLTSPDQLYRA
                     GLDGASPVALTTHNAATLAQIQFGAVEQFSFPGWNGDTVYGHVVKPWNYVKGKKYPVA
                     FIVHGGPQGSMGNDWHYRWNPSIYAGWGYAVVFIDFHGSTGYGQAFTDAISGHWGDRP
                     LEDLQKGWAYALQKYDFLDARHAAALGASYGGYMMNWIAGNWQDAFQCLVSHDGVFDN
                     RMMNYSTEELWFDEWENGGTQYEKPEAYEQFNPINHVAQFKTPMLVIHSEKDFRIPAE
                     QGIGLFTALQRRGIPSQLLIFPDENHWVLKPQNSMQWHDTVHAWLQKWIGG"
BASE COUNT         1134 a         2419 c         2520 g         1315 t
ORIGIN      
        1 tcgaatttgc gccggaagcg catgagggcg accctgccgt gttccaggcg cgcgccgccg
       61 cgctgcccgc gttgccgccg tcgccggtgc agaaggccga catcgtggtg tggctcaacg
      121 agtcgacctt cgatccgcgt aattaccacc tgcccggcgc gcgtctgccg catctgcgca
      181 tgatggacgc ggcaccgacc acgcgcgccc gtgaccacct gcgcgtgcac acctacggcg
      241 gcaagacctg gctgtcggag ttctccctgc tcaccggcct ggtgccggac gacttcggcg
      301 cgcggcgcaa cctggtgttc aactcggtca cgcccaatac caccagcaac ctggtgcgcg
      361 cgctcaaggc cgacggctac aagacggtgg tgctgatgcc gaccttcaag cgcttctacg
      421 gcgcaggccg cacctacctg ggcaccggct tcgaccaggt gctgacgctg cgcgattttc
      481 ccgaatacga caacgtgccg ggagacgagt gggacatcgc cgacagcgac cgcctggccc
      541 aggcggcaca gaccatcgtc gagcgtcacc gggcaggtcc agacggcgcg cagccgctgc
      601 tgctctacct gctgtcgatc aaggagcatg cgccgtactc gaagaaaacg ccgattgact
      661 acgccttgca gcgcgtgccg atcaaggaat cgctgcagca caagttgtcc gattatctcg
      721 gcaagctggt caagctggac aacgcgctgt tggcattaca gcaggcgctg ttccgcaacg
      781 gtgagcggcc ggtggtattc gcctggttcg gcgaccacca ggcttatttc gaggaagatg
      841 cgccacccta cacctacgat ctgcccaagc ccgattacct cacgcagttc cagctgcgca
      901 ccaacttcaa cgcgccagcg acggctacgg gcgactattt cgatatcgcg ttcctgccca
      961 gcctgctgat cgattacgcc ggggcaaggc ccgacgacta tttcgccggc ctgtcggcga
     1021 tgcgccggct gtgtgggggg ctgatggacg agtgcgccga caaggcgctg gtgcagagct
     1081 acaaggcctg ggtattcagc cccggcctcg gattatttgc gcagtaggct gacgcgctgg
     1141 ctgctggccg cggcaccgcc gcgcagttcg atcaggccgt gatccaccag ttcctgcagc
     1201 atgccgctga tgccgggcat gcgcgcgaac agttcggtca ttgaacgcac cgtcttctgg
     1261 ccatcgctgg cgatcagcag cgtgcgcgcc gcctgcgaca gcgccagcgt gcgtgtcttg
     1321 atttcctcgc gacccttgtc ggtcttgacc agtacctggt ccggttccat cgcttcctcc
     1381 cggcccttgc gggccttgcg ccgtggcgct catcaacgtc ccgtctgcgc gggttaatgc
     1441 aaggttagcg caatgctgcc ctcagcgaaa tggggctttt ccccctgcgc cgcgctctct
     1501 aaagtagcgc gcaatttcca gcatcaggac cacgggaacc ccttgaacat gccagcacac
     1561 agcccggcca ggaccatcgt catcaccggc ggttccagcg gcatcggcgc cgaggctgcg
     1621 cgccagctgg cgcggcaggg tcacacgctg tgcctggtgg cgcggcggcg cgaggagctt
     1681 gaaaaggtgc aggcggagat cgcggccgaa ggcggcaagg cgcacatcta tgcggctgac
     1741 ctggccgatg gtgcggccgc cgatgcctgc tgcaagacga tcctggctga gcatgctcgg
     1801 gtcgacgtgc tggtcaacaa cgccgggcgc tccatccgcc gcccgctgct cgaatcgctg
     1861 gaacgcgcgc acgatttcga gcggaccatg cagatcaatt atttcgccgc cgtgcgcatg
     1921 acgctcggcc tgctgccgcg catgctgcag cagaaggacg gccacatcat caacatctcc
     1981 agcgtggtgg cgctgatgcg tacgccgcgc tttgccgcct acgtgggcag caagtcggcg
     2041 ctggacgcgt tcggccacac gctggccatg gaactgcacg acgccggcat ccgcgtcagc
     2101 accgtcaact tcccgctggt gaagacggcg atgaccgcac cgaccgcggc ctacaattat
     2161 gtcgagcaga tggacgtgcg ccatgccgcg ggctggatcg tcgatgccgt gcgcacgcgc
     2221 cgcgtgcgac gtaccacctg ggatgccgtg ctcggcatca ccgccatcgg gctcgcaccc
     2281 gggctggcgc tggccacgct gcggcgctac tacaagcgac gcgtgcggct gatgcagaag
     2341 cgcatgcagg aggccgcgca gtcgcagcaa tcgtcctgag gttgtcatcc ccggcgccgc
     2401 ggagcttgtg catgcgcggc gcccggaatg acaacgggtg atgcatgaaa ttttccgggt
     2461 tgatgaattc ggccgtggcg gatggccatg cgctgtcggt ggatgtgggc gaggactggc
     2521 tgcagggccg cagcgtgttc ggcgggctgc aggccgcgct catgctggcg gccatgcgcc
     2581 ggctggtgcc ggcggcactg ccgctgcgca ccttgcaggt caccttcatc gcgccgatcg
     2641 gggctggaac cctgcgcgcg caggccacgc tgctgcgtac cggcaagagc gccatgcatg
     2701 tgcaggcggt gctggccgat cccgccggcg ccacgctatg cctggcggcg gcggtgttcg
     2761 gcgcgtcgcg cgaatcgcag gtgcgcgtga cgccgcagcg gccggcgata cctggcggta
     2821 caccgatcga tttccgctac gtgcagggcc tgacgccgaa cttcacgcgg cattactcga
     2881 tgcgctggct gctcggcaac gtgccgttct ccggcacgcc aggcgagcgc gcggtgatcg
     2941 aagtgggcgt gcacgatgac ggcgcggcga gcgaggggca cctgctgggc attgccgatg
     3001 tcattccgcc ggtcgcgctg tcccgcttcc gccagccggt gttcggcagt tcgctgacct
     3061 gggccatcga tttcctgctc gaccgctacg acacgcagcc gctcaccggc tggcgcctgg
     3121 atgcggaact ggtcggcgcg cgcgacggct acaccagcca ggacgtccac gtgtgggcgc
     3181 cggacggcca gctggctgcg ctgtcgcgcc agtgcatggt ggtgttcggc tgacgcgaac
     3241 tacgccgcgg gaaaacgcgg cgccatcgcc tcgcgttcca ggcgcgtggc gttgatcagg
     3301 tacgacaccg cgcggtacag cccggccagc atcaccagtt cgatcagctg gtcgtcggcg
     3361 taaaccgact tgagctcgcc ccacagggcg tcgttgacgt cgtgcttctc gtgcagcgca
     3421 tcggcgagct tcaccaccgc ggcgtcttca cgcgtccaca ccggcgcagc ggcgtcgccg
     3481 tgcaccgatg catgcagctg ttccggcgtg aagccggcct tggtgttgta ggcggccaca
     3541 tgcacgcccc attcgtactc ggcgccgcag cgcgcgcagg tgcgcaggat catcagctcg
     3601 cgcgcgcgca ggctgatgct gccgcggtcg agcaggccgc cggcgatcag gcgcgacagc
     3661 acgcgcgggt tgcgccccac cgtgcggaac agcttgagtg gcggcagccc gcgcatcacc
     3721 ttgtcgaaat cggcctgcat ctcggcggtg tagggcgggt ccagcggggc gatgcgggca
     3781 ctggtcatgg cgaacttacc ttgctaggat gggcttgcta cgattttagt agcaagcgct
     3841 tcgaaaaagg tagcagatca tgtcccccat gcctggaaac aaagtcagag gctcgcgcag
     3901 cggccggccg atcatggcgt tgcttgacct gcttggccgc cgctggaaca tgcggttgct
     3961 gtgggaactg cgcggcgagc cgctcaactt ccgcgcgctg cagcaggcct gcggcgggct
     4021 gtcgccctcg gtgctgaata cgcgcctggc ggaactgcgc caggcgcagc tggtcgatgg
     4081 cggcgacgcc ggctacacgc tgagccggca ggggcaggcg ctgctcaagc agttcgcgcc
     4141 gctcaaccgc tgggcggaag gctgggcggc cggcctgggc aaaaagcgcg gaaagtagcg
     4201 gcgctcagaa gcgccagcgg aacgagagct gcaccacgct ctcggtgccg ccgttgaacg
     4261 gatcgttgtt gttccagcgc gcgcgctgca gcagcgcctg caggcggccg ggcgcgccga
     4321 ccgcgcggcc gaggttgacc gtcagctgcg gcgccacttc caggcggcgg ccgccgtcga
     4381 gttcgcgcac cacgttgacc gcgccgccca ccgaccacag cggttcggcg ctgccgtcat
     4441 cgctgtcgtc gggcgccagt tgcacgcgct tgccgcggct gcgcggattg ctgcgcgtgt
     4501 agaggttcag gtacaggttg gtcacgtcgg gcactttcag gtccaggccg acgccgtcct
     4561 tcatctgtgc cagctcgtcg accagcgtgg ccgtgagcag cggcgcatcc tggccgatgg
     4621 ccaccgggtg cagggcgaga tcgctgccgc tgcgatgcgc gcgcggatcg agcgggtcgg
     4681 gctgcgcggt gagcgttacc gggtagccgg cggccagcca ttgcgcggcc agcgggttga
     4741 ccatgctggg cgccggcacc ggccgcgtcg cgcgcgtgcc gggttcgctg gccgcggcat
     4801 gaccggcata ggccagagcc aggccgtaga gtgcccgccg ggtccagttg atcacgcctt
     4861 tgcgccggcg gttcctgccg gagaactcct gttgcacgac gcgccaccgc acttggtttc
     4921 gatgcctgat tatcgggacg ctctgcgcag aggcccctag agcaatcatc tatacggggg
     4981 ctccgcgcga ccggcggtag cgcgaaagcc gcaggcggcg gctggaactt gaatcggcta
     5041 gattgcgcgc cctatgtcga tcgggatcgg ggaaatctat tcgctgggct gcgcgctggc
     5101 ctgggccatc gccgtcatcc tgttcaagaa gtccggcgag agcctgccgc cgctggcgct
     5161 caacctgttc aagaacgcac tgtcgctcgt gatgatcggt gccaccatcg tgctgctgcg
     5221 cggaccgcag ctgccgcaga ttccgccgat ggcgctcggg ctgatcctgc tctcgggact
     5281 gctcggcatc gccgccggcg acacctatta cttccaggca ctcaacgcca tcggcgccgg
     5341 gcgcatcgcg gtggcgcaga cgctgtattc gccgttcgtc atcaccttgt cgatgacctt
     5401 tctcggcgag cacctgcgcc cgctgcagtt cggcggcgtg gcactggtgc tcggcggcat
     5461 cgtgctggtg acttacgtgc gcgagcgcaa tcacccggat gcgcacaggc tgcgccgcgg
     5521 cgtgctcacc gcggtgctcg ccatgttctg cacggcggtg ggcatcgtca tggccaagcc
     5581 gatgctggcg cagcacgatt tcctgtgggt ggtgtggctg cgcattgccg gcggtttctt
     5641 cggcctgtgc gcggtggtgc tggcgcgcgg cgatgcgggc gagatgcgcg cgcggtgtgc
     5701 gcgcgtgcgc cactggccgc aggtgatcgg cggcacggtg atgggtaact acgtgtcgat
     5761 gatgctgtgg ctgggcggct acaagtacac caaggcctcg atcgccgcgg tgttgaacga
     5821 actggccgcg gtgttcatcg tgctgttcgc cgtgctgttt ctgcacgagc gcgccagcgc
     5881 gcggcaggtg gctggcgtgc tgctggcggt ggccggcgtg gtgatggtgg tggcgctgtg
     5941 aggccgattt ttgctcatac cgcaccccgg cctggcatgt tataaattat ttatcgaaat
     6001 agaaataaga taatatttta taactcgcta aaccggggtg cggttgaaga aaaaatcagc
     6061 cgccaatcca cttctgcagc cacgcgtgca ccgtgtcatg ccactgcatg ctgttctgcg
     6121 gcttgagcac ccagtggttt tcgtcgggga agatcaggag ctggctggga atgccgcgcc
     6181 gctgcagcgc ggtgaacagg ccgatgccct gctcggcggg gatgcggaaa tccttctcgc
     6241 tgtggatcac cagcatcggc gtcttgaact gtgccacgtg gttgatcggg ttgaactgct
     6301 cgtaggcctc gggcttttcg tattgcgtgc cgccgttctc ccactcgtcg aaccagagtt
     6361 cctcggtgga gtaattcatc atgcggttgt cgaacacgcc gtcgtggcta accaggcact
     6421 ggaacgcgtc ctgccagttg ccggcgatcc agttcatcat gtagccgccg tacgacgcgc
     6481 cgagcgcggc ggcgtgcctg gcatcgagaa aatcatattt ctgcagcgcg taggcccagc
     6541 ctttctgcag gtcttcgagc gggcgatcgc cccagtggcc ggaaatggcg tcggtgaacg
     6601 cctggccgta gccggtggag ccgtggaagt cgatgaacac cacggcgtag ccccagccgg
     6661 cgtagatcga cgggttccag cggtaatgcc agtcgttgcc catgctgccc tgcgggccgc
     6721 cgtgcacgat gaacgcgacc gggtatttct tgcccttcac gtaattccac ggcttcacca
     6781 cgtggccata cacggtgtcg ccgttccagc cggggaagga gaactgctcc acggcgccga
     6841 actggatctg cgccagcgtg gccgcgttgt gcgtggtcag cgccaccggg ctggcgccgt
     6901 cgagcccggc gcggtacagc tggtcgggcg aggtgagcga gttctgcgca tagacgatgc
     6961 cgtcggccgc cagcgtgaag ccgctgaccg tgccctggcc gctgatgcgc gtgacgttgc
     7021 cggtggcgat atccaccgcg aacagcggcc gctggccgat gtcgtcggcg tagcagtaga
     7081 tgcttcttcc gtcgggcgac agcgccagcg cgtcggggct gcgatcccac ctgggcgcga
     7141 tctcgcgcac ggcgccggtc ttcaggtttt tctccatcag ccagaagcgg tcggcctcga
     7201 agccgggacg cttcatcgcg cggtagtaga gcgtgttgcc gtcggccgag aacagcggcg
     7261 aggtgtccca cgccgggttg ctcgcggtga gattttccgg agccttcgag ccatccgccg
     7321 gcacgcggta gatatcgaaa ttggtgctcc acggctcggt cttgccggcg atgcgcaccg
     7381 agaaggcg
//
LOCUS       DASZZC010000620         6665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1075586,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000620 DASZZC010000000
VERSION     DASZZC010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075586"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..574)
                     /locus_tag="VHE37_13225"
     CDS             complement(125..574)
                     /locus_tag="VHE37_13225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT36543.1"
                     /translation="MKKDFTYYLRVRYGECDAQKVVFNARYAEYTDLAVTEFFRALGY
                     GEQLASGELDYQLVRQTLEWKAPARFDQVVAISVRALKLGTTSFTLRCEFFIAGSEPL
                     IASAETVYVLVDTRTLTKRTLPAPLRAAMERGAPGRETDHAQFRTRT"
     gene            777..3383
                     /locus_tag="VHE37_13230"
     CDS             777..3383
                     /locus_tag="VHE37_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT36544.1"
                     /translation="MHAVSQGPFTRFFLRFTEPVVFGKRHITLAVLALVTALMALQAS
                     RLRPDTGFEKQLPLQHEYMKVFKRYQSQFGGANLVLIAAVQHQGDRASDNDIYNPRFL
                     GTLKQLTDEVFFLPGIDRARVSSLFTPDVRYIEVVEGGFSGGNVIPANYSPTPETLAQ
                     IRRTVARANIRGRLVSNDQRGAMVFAELLESDPISGEKLDYADAAHRLENLRGQFGQP
                     WEYVLKLKGAQEPFAAGEVLAREYGADEQAPRAHGVNAIHLDPYGLPTRRHFSAGDID
                     VEVKKNADYNPDVDLHIIGFAKVVGDVSDAAAEVVGFFGLTLLITLALLWMYCGSLRI
                     ALLPLSCALLAVIWELGLLKTFGFGLDPFAILVPFLVLAVGVSHGVQITNFWLYEMTD
                     HGRTSFEASRATFRRLVIPGITALLTNIVGFGTILLIPIGIVQEMAWNACFGCFAIII
                     TKKIVLPILLSYVQVSDLQKFRDHQRRRDALFAAPWRAVARITDRPVAIGVLVAALAL
                     YGCSEWLARDLKMGELHAGVPELRPDSRYNLDNAAIGRDFAVGTDIIKVIAQTHAQGC
                     IEYPVMQTIDDFAWRLTNTPGVQQTFSLPQAGRMVDAAFNEASPKWLVLPRNTATMVQ
                     SITPFTTSTGLLNEDCSAMPVLIFTEDHKAETISRVVDATKQFEAELPKDSPVHFALA
                     SGNVGVMAAANEVIKATERPMLFWVYAAITLCVWLSFRNFTSVICILLPLALVSVLSY
                     AVMVLLGIGLKVATLPIAAFAAGIGVDYGIYIYSVLEEQVAKGLSLRRAYEETLHQTG
                     KAVVFTGLALGASVCTWLLSDLQFQVDMGALLTVMFLANMVGAVVLLPALASFLVKPQ
                     AAEPDGEPEVAT"
     gene            3486..4622
                     /locus_tag="VHE37_13235"
     CDS             3486..4622
                     /locus_tag="VHE37_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HVT36545.1"
                     /translation="MTDTQTPASADGSAGTAAVEPGAVLASEHTSNLTGIFEQLGISL
                     LVTTYQAGKLIAVRREGAALNTHFCAFNKPMGLAADPTRFALGTAREISQYRNMPAVC
                     AKLEPKGAHDACFVPRDAHVTGDIDIHEMAYAKNGELWFVNTRFSCLCTLDGISSFVP
                     RWRPKFVSSLAPEDRCHLNGLGLVDGEPRYVTALGQADKPAGWREHKASGGVLIDIKS
                     GKLLCDDLSMPHSPRWYADQLWLLESGKGTLSRVDPKTGRREVVVTLPGFTRGLDFFG
                     PLAFVGLSQVRETAVFSGIPITEKAGERICGVWVVNILTGQIVGFLRFKSGVQEIFAV
                     SVLPGIRFPEILEPGHELVATSYALPDAALKDVRLSSPRPPSQA"
     gene            complement(4641..5624)
                     /locus_tag="VHE37_13240"
     CDS             complement(4641..5624)
                     /locus_tag="VHE37_13240"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HVT36546.1"
                     /translation="MNADELQDLKIRAQSGDVSAMFSLGSLVVSGVDAGMPPRQGVYL
                     LTDAAQRGEAAAAHLLAVLSAAGRFGLPNWPQALDHLARAAELGSAYAAQQLVLLAGP
                     QPVAVRGWRALRNCIDVQAWRTPPAKEVVCEAPRIRKANGFLPAAACDWLIWRATARL
                     EPAQQRADSADPAGLHEMRTSSEAEFSLLDTDLVMLLTQERIAAITGVPREVMEPPRV
                     LQYRAGEEFALHHDFLDPQDPALKDELQRNGQRIVSFLLYLNDDYEGGETDFPVARLR
                     HKGRKGDALYFANITDGDVPDHRTLHAGLPPTRGAKWLLSQWMRSRTPARI"
     gene            complement(5621..6124)
                     /locus_tag="VHE37_13245"
     CDS             complement(5621..6124)
                     /locus_tag="VHE37_13245"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT36547.1"
                     /translation="MDILQTERLCLRPLDAGDAAFMLELLNDPAWLRNIGDRGVRTLE
                     QARDYITQPPASGVQRLQFCIELRTGGAKVGVAGIYRRPTLDQPDLGFALLPAYRGCG
                     YALEAARAVLAHARGALDVRHIAAIAAPGNTASHVLLEKLGLHYQRRIELGAESLCYF
                     AGETGAA"
     gene            complement(6133..>6665)
                     /locus_tag="VHE37_13250"
     CDS             complement(6133..>6665)
                     /locus_tag="VHE37_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36548.1"
                     /translation="SLAINPVRNARVADAADLTERQADLPMVLAEPVSRDAALAESYR
                     RLLYAARAEPATLSGELLTEAQAQQLKQHLGSPVVMVVMLWGQSVSTAVDVPKVSGCA
                     VFASVCGRGFTPDLDKSRALASVFDLDARAVIWTQVIRFPLGDASLLFAGGDPARMDN
                     YSGLGWMEDLTRPFFR"
BASE COUNT         1106 a         2229 c         2207 g         1123 t
ORIGIN      
        1 gcccagcgcc atggacaaca gcatcgctcc gcatcctgca atcagcttgg tacgcatggg
       61 tcgcttctta ggtggtgacc gcacagcccg tgtctgcggc cagtatatcg gcgccgctcg
      121 ccggtcaggt gcgagtgcgg aattgcgcgt ggtcggtctc gcgcccgggc gcgccgcgct
      181 ccatggccgc gcgcaagggc gcaggcaagg tgcgcttggt cagcgtgcgc gtatcgacca
      241 gcacgtaaac cgtctcggcg ctggcgatca gcggctcgct gccggcgatg aagaattcgc
      301 agcgcagcgt gaacgaggtg gtgccgagct tgagcgcgcg cacgctgatc gccaccacct
      361 ggtcgaagcg cgccggcgcc ttccactcca gggtctgcct gaccagctgg tagtcgagtt
      421 cgccgctggc cagctgctcg ccatagccca gcgcgcggaa aaattcggtg acggccaggt
      481 cggtgtactc ggcatagcgc gcgttgaaca ccaccttctg cgcatcgcac tcgccgtagc
      541 gcacgcgcag gtagtaggtg aaatcttttt tcatcgcatc tctggaaaat cagtccaccg
      601 caccgctatt ttgcctgtta ttaattataa catgcttata attctaaaca ttaatttgta
      661 acaccataaa ccctggtgcg gtggagcggt tgcaggccca ccctgtgcta agttcgggcc
      721 acaagcagtc cgcgccacgc agcgcgggca tacgcgcatc gaggggagag agattcatgc
      781 acgccgtgtc gcaaggaccg ttcacgcgtt ttttcctgcg tttcaccgag ccggtggtgt
      841 ttggcaagcg ccacatcacg ctcgccgtgc tggcactggt caccgcgctg atggcgctgc
      901 aggccagccg cctgcggccc gacaccggtt tcgaaaagca gctgccgctg cagcacgaat
      961 acatgaaggt gttcaagcgc taccagagcc agttcggcgg cgccaaccta gtgctgatcg
     1021 ccgccgtgca gcaccagggt gaccgcgcga gcgacaatga tatttacaac ccgcgcttcc
     1081 tgggcacgct caagcagctc accgacgaag tgttcttcct gcccggcatc gaccgcgcgc
     1141 gcgtgtccag cctgttcacg cccgacgtgc gctacatcga ggtggtggaa ggcggcttct
     1201 ccggcggcaa cgtgattccc gccaactatt cgcccacgcc ggaaacgctg gcgcagatcc
     1261 gccgcaccgt ggcgcgcgcc aacatccgcg gaaggctggt atcgaacgac cagcgcggcg
     1321 ccatggtgtt cgccgagctg ctggagtccg acccgatcag cggcgagaaa ctcgactacg
     1381 ccgatgccgc gcaccggctg gaaaacctgc gcggccagtt cggccagccc tgggaatacg
     1441 tgctcaagct gaaaggcgcg caggagccgt tcgccgccgg cgaagtgctg gcgcgcgagt
     1501 acggtgccga tgaacaggcg ccgcgcgcgc acggcgtgaa tgccatccat ctcgatccgt
     1561 acgggctgcc cacgcggcgg catttctccg ccggcgacat cgatgtcgag gtgaagaaga
     1621 atgcggacta caacccggac gtcgacctgc acatcatcgg cttcgccaag gtggtgggcg
     1681 acgtgtccga cgccgcggcg gaagtggtcg gcttcttcgg cctgaccctg ctcatcaccc
     1741 tcgccctgct gtggatgtac tgcggttcgc tgcgcatcgc gctgctgccg ctgtcatgcg
     1801 cgctgctggc ggtgatctgg gaactgggcc tgctcaaaac gtttggattc ggcctcgacc
     1861 ccttcgccat cctagtgccg ttcctggtgc tggcagtggg cgtgtcgcac ggcgtgcaga
     1921 tcaccaattt ctggctgtac gagatgaccg accacggccg caccagcttc gaagcctcgc
     1981 gcgctacctt ccgccggctg gtgattcccg gcatcaccgc gctgctgacc aacatcgtcg
     2041 gcttcggcac catcctgctg atccccatcg gcatcgtgca ggagatggcg tggaacgcct
     2101 gcttcggctg cttcgccatc atcatcacca agaagatcgt gctgcccatc ctgctgtcgt
     2161 acgtgcaggt cagcgacctg cagaaattcc gtgaccacca gcgccggcgc gacgcgctgt
     2221 tcgccgcgcc gtggcgcgcg gtggcgcgca tcaccgaccg gcccgtcgcc atcggcgtgc
     2281 tggtcgccgc actggcgctg tacggctgca gcgaatggct ggcgcgtgat ctgaaaatgg
     2341 gcgaactgca cgccggtgtg ccggagctgc ggccggattc gcgctacaac ctcgacaacg
     2401 cggcgatcgg ccgcgacttt gccgtcggca ccgacatcat caaggtgatc gcgcagacgc
     2461 atgcgcaggg ctgcatcgaa tacccggtga tgcagaccat cgacgacttc gcctggcgcc
     2521 tgaccaacac tcccggtgtg cagcagacct tctcgctgcc gcaggccggg cgcatggtcg
     2581 acgccgcgtt caacgaagca agccccaagt ggctggtgct gccgcgcaac accgccacca
     2641 tggtgcagtc gatcacgcca tttactacat caaccggttt gctcaacgaa gactgcagcg
     2701 ccatgccggt gctgatcttc accgaggacc acaaggccga gaccatcagc cgcgtggtcg
     2761 acgccaccaa gcagttcgaa gcggagctgc ccaaggacag cccggtgcat tttgcgctgg
     2821 cctccggcaa cgtcggcgtg atggccgcgg ccaacgaggt gatcaaggcc accgagcggc
     2881 cgatgctgtt ctgggtctac gccgcgatca cgctgtgcgt gtggctgtcc ttccgcaact
     2941 tcaccagcgt gatctgcatc ctgctgccac tcgcgctcgt ctcggtactg agctacgcgg
     3001 tgatggtact gctcggcatc ggactgaagg tggcgacgct gccgatcgcc gccttcgccg
     3061 ccggcatcgg cgtggactac ggcatctaca tctacagcgt gctcgaagaa caggtggcca
     3121 aagggctgtc gctgcgccgc gcctacgagg agacgctgca ccagaccggc aaggcggtcg
     3181 tgttcaccgg gctggcgctg ggcgcttcgg tctgcacctg gctgctgtcc gacctgcagt
     3241 tccaggtgga catgggcgcg ctgctcacgg tgatgttcct ggccaacatg gtcggcgcgg
     3301 tggtgttact gccggcgctg gcctcgttcc tggtcaagcc gcaggccgcg gagcccgacg
     3361 gcgaacccga ggtggcaacg taaggccgga tgccgggttc accaggtggc gatgttcctg
     3421 gcctgcattg atcgcggcgc ggtggtgttg ctgccggctg acaggccctc gcatatagtg
     3481 gcgccatgac cgacacccag actccggctt cggccgatgg cagtgccggc acggcggcgg
     3541 tcgagccggg cgcggttctt gccagcgagc acacttccaa tctcaccggc attttcgagc
     3601 agctcggcat ctcgctgctg gtgacgacct accaggccgg gaaactgatc gccgtgcggc
     3661 gggaaggcgc cgcgctcaac acgcatttct gcgccttcaa caagccgatg gggctggccg
     3721 cggatccaac acgcttcgcg ctcggcacgg cgcgggagat ctcgcagtac cgcaacatgc
     3781 cggcggtatg cgccaagctt gaacccaagg gagcacatga tgcctgcttc gtgccgcgcg
     3841 acgcgcatgt caccggcgac atcgacatcc acgagatggc atacgccaaa aatggcgagt
     3901 tgtggttcgt caacacgcgg ttctcctgcc tgtgcacgct cgacggcatc agcagcttcg
     3961 tgccgcgctg gcggccgaag ttcgtcagtt cgctggcgcc ggaggaccgc tgtcatctca
     4021 acggtctcgg gctggtcgac ggcgagcccc gctatgtcac cgcgctcggg caggcggaca
     4081 agccggccgg ctggcgtgag cacaaggcca gcggcggcgt gctgatcgat atcaagagcg
     4141 gaaaactgct gtgcgacgac ctatccatgc cgcactcgcc gcgctggtac gccgaccagc
     4201 tgtggctgct ggaatcgggc aaaggcacct tgtccagggt tgatcccaag accgggcgtc
     4261 gcgaggtggt ggtaacgctg ccgggtttca cccgcggcct ggatttcttc ggaccgctcg
     4321 ccttcgtcgg actgtcgcag gtgcgcgaga cggcggtgtt cagcggcata cccatcaccg
     4381 aaaaagctgg cgagcgcatc tgcggcgtgt gggtggtgaa catcctcacg ggacagatcg
     4441 tcggattcct gcgcttcaag tcgggcgtgc aggaaatctt cgcggtgagc gtgctgccgg
     4501 gcatccgctt cccggaaatt ctcgaacccg ggcacgagct ggtggccacc tcgtacgcgc
     4561 tgcccgatgc ggcgctcaag gatgtgcggc tgagctcacc aaggccaccg tcccaggcct
     4621 gaactcaccg cggaatctgg tcaaatcctc gccggcgtgc ggctgcgcat ccattgcgac
     4681 agcagccact tggcgccgcg cgtgggcggc agcccggcgt gcagcgtgcg gtgatcggga
     4741 acatcgccgt cggtgatgtt ggcgaagtac agcgcatcgc ccttgcgccc cttgtgccgc
     4801 aggcgcgcca ccggaaaatc cgtctcgccg ccttcgtagt cgtcattgag gtacagcagg
     4861 aagctgacga tgcgctggcc attgcgctgc agctcgtcct tcagcgccgg gtcctgcggg
     4921 tcgaggaagt cgtgatgcag cgcgaactcc tcgccggcac ggtactgcag cacgcgcggt
     4981 ggctccatca cctcgcgcgg cacgccggtg atggcggcga tgcgctcctg cgtgagcagc
     5041 atcaccaggt cggtgtcgag caggctgaac tcggcttcgg agctggtgcg catctcgtgc
     5101 aggccggcgg gatcggcgct gtcggcgcgc tgctgcgcgg gctcgaggcg cgcggtggca
     5161 cgccagatca gccagtcgca tgctgcggcc ggcaggaaac cgttggcctt gcggatgcgc
     5221 ggcgcctcgc agaccacttc cttggccggc ggcgtgcgcc aggcctgcac gtcgatgcag
     5281 ttgcgcaacg cgcgccagcc gcgcaccgcc accggctgcg gaccggcgag caggaccagt
     5341 tgttgcgcgg cgtaggccga gccgagctcg gcggcacgcg ccaggtgatc cagcgcctgc
     5401 ggccagttgg gcaggccaaa gcggccggcg gcgctgagca cggccagcag gtgcgccgcg
     5461 gcggcctcgc cgcgctgcgc tgcatccgtg agcaggtaga cgccctggcg cggcggcatg
     5521 ccggcgtcga cgccgctcac caccagcgag ccgagcgaga acatggccga cacgtcgccg
     5581 gactgtgcgc ggatcttgag atcctgcagt tcgtcggcgt tcatgcggcg ccggtctcgc
     5641 cggcgaaata gcagagcgat tcggcgccga gctcgatgcg gcgctggtaa tgcaagccga
     5701 gtttttccag cagcacatgc gaggcggtgt tgcccggcgc agcaatggcg gcgatgtgcc
     5761 gcacgtccag cgcaccgcgc gcatgcgcca gcacggcacg cgcggcctcg agcgcgtagc
     5821 cgcagccgcg atacgcgggc agcagcgcga agccgagatc aggctggtcc agcgtggggc
     5881 gccgatagat gccggccacg ccgaccttgg ctccgcccgt gcgcagttcg atgcagaact
     5941 gcagccgctg cacaccggac gccggtggct gcgtgatgta gtcgcgcgcc tgctccagcg
     6001 tgcgcacgcc gcggtcgccg atgttgcgca gccatgctgg atcattgagc agttcgagca
     6061 tgaacgcagc atcgcccgcg tcgagcggac gcaggcacag ccgctcggtc tgcaggatgt
     6121 ccatgcgcgt gctcagcgga agaacggccg cgtcaggtct tccatccagc cgaggccgga
     6181 atagttgtcc atgcgtgccg gatcgccgcc ggcgaacagc agcgaggcgt cgccgagcgg
     6241 aaacctgatc acctgcgtcc agatcaccgc acgcgcgtcg agatcgaaca ccgaggccag
     6301 cgcgcggctc ttgtcgagat cgggcgtgaa gccgcgcccg cacacgctgg cgaatactgc
     6361 gcagccggac accttcggca catcgaccgc ggtgctgacc gactggcccc acagcattac
     6421 caccatgacc accggcgaac ccaggtgctg cttcagctgc tgcgcctgcg cttcagtgag
     6481 cagttcgccg ctgagcgtgg ccggctcggc gcgcgcggcg tacagcagcc gccgatagga
     6541 ctcggccagc gcggcgtcgc gcgacaccgg ttcggccagc accatcggca gatcggcctg
     6601 gcgctcggtg agatcggccg catcggcgac gcgtgcattg cgcaccgggt tgatggcgag
     6661 actgc
//
LOCUS       DASZZC010000621         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_374435,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000621 DASZZC010000000
VERSION     DASZZC010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374435"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHE37_13255"
     CDS             complement(<1..392)
                     /locus_tag="VHE37_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVT36549.1"
                     /translation="MNENFYTLYAWPMSYYSGKSRAYLRYKAVPHAEKPLRIWTFRKV
                     KRKTGATVMPVVVTPDGDWLQDTSHIMDVLEPRFPRAPVLPATPRQRVAALILEAWGD
                     EFWLPSAMHYRWSFPENFRTVFQPEGGD"
     gene            complement(397..1080)
                     /locus_tag="VHE37_13260"
     CDS             complement(397..1080)
                     /locus_tag="VHE37_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVT36550.1"
                     /translation="MIELYTAPTPNGHKISVCLEELALPYTVHVVDITRGDQRKPEYV
                     KLNPNARIPTIVDTEEDNFAVFESGAILIYLAEKTGRLMPSDAKGRSLVMQWLMFQMG
                     GIGPMMGQANVFFRYFPEKIQPAIDRYHHECRRLFEVLDRRLGESQWLAQDFSIADIA
                     NWAWVRTYKWSGVSIDGLQHLQRWNDAMYARPACKKGLEVPFKAPKLESDAKAAEAFI
                     KGAQNIVQR"
     gene            complement(1077..2264)
                     /locus_tag="VHE37_13265"
     CDS             complement(1077..2264)
                     /locus_tag="VHE37_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HVT36551.1"
                     /translation="MSGDWIIWGSELSPFTLKLIRLFRHARLPLRFLPAEGSFTENWH
                     YALRVDRLKRGMLPLTCPAMTEDDEFPLVPFVFGPEGENLYDSSAIARWLDARLEPAL
                     RVIPGAEAASFVARLIDDYADEWGLYLVHHNRWKVSALDNDAGTRVAREFRFTSLGLL
                     QFAGARWFAARQTRRLAYLFSVAPEGFHINGLPASRQPPSRAGFPPTHELLEHAHLRL
                     LDILETLLANRPYLLGGMFTLADAAIYGQLGMNLSDPSADRLMRERAPHVHAWLTALH
                     GADPSPIPGAAENIQLDAGLKPLLAEICRTHVALMRQNAEAVARLHVQGQRRFNERAF
                     NRGEALYDGVIDGFPFRHVAKTFQARVWRDCLTHRSRLSSAARAQVDALLPANHGIDG
                     VAA"
     gene            complement(2271..3347)
                     /locus_tag="VHE37_13270"
     CDS             complement(2271..3347)
                     /locus_tag="VHE37_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT36552.1"
                     /translation="MLKKILLTALAIIVVAAAAGALMRDRLVEAAMRKQIMRNLSGEA
                     FREMNDGLNVVLCGAGSPLPDPTRSGPCVLVIAGTRVMVVDVGSGAVRRIGPAGVPMA
                     RIEDVFLTHFHSDHIDGLGELMMQRWAGASRTSPLPVHGPTGVEDVVAGFNQAYMHDD
                     HYRVAHHGEKIIPPSGAGGIAMPFELPAEGQGTVVLDTDGLKVTAFAVDHRPIVPAVG
                     YRFDYQGRSVVISGDTVKSANLQHFAQGADLLVHEALNPDLLKIMTNAAVDAGAANLA
                     QITRDIVNYHTTPVQAAEIARDAGVKHLLFYHLVPALPYRPMEQMFVRGVSGIYKGGV
                     TVGHDGTWISLPANSSDVRVGSRF"
BASE COUNT          572 a         1086 c         1170 g          591 t
ORIGIN      
        1 tggtcgccgc cttccggctg gaacacggtg cggaaatttt ccgggaagct ccagcggtag
       61 tgcatggccg agggcagcca gaattcatca ccccaggctt ccaggatcag cgcggccacg
      121 cgctggcgcg gagtggcggg cagcaccggc gcgcgcggga agcgcggctc cagcacatcc
      181 atgatgtgcg aggtgtcctg cagccagtcg ccatcgggcg tcaccaccac cggcatcacg
      241 gtggcgccgg tcttgcgctt caccttcctg aacgtccaga tgcgcagcgg cttttccgcg
      301 tgcggcaccg ccttgtagcg caggtaggcg cgcgacttgc cgctgtagta cgacattggc
      361 caggcgtaca gcgtgtaaaa attttcgttc atggttttac cgctgcacga tgttctgcgc
      421 gcccttgatg aaagcctcgg cggccttggc gtcggattcg agtttcggcg ccttgaacgg
      481 cacctcgaga cctttcttgc aggcagggcg cgcgtacatg gcgtcgttcc agcgctgcag
      541 atgctgcagg ccgtcgatcg acacgccgga ccatttgtag gtgcgcaccc aggcccagtt
      601 ggcgatgtcg gcgatggaga aatcctgcgc cagccactgg ctttcaccga gcctgcggtc
      661 gagcacctcg aacagccgcc ggcactcgtg gtgatagcgg tcgatggcgg gctggatctt
      721 ttccgggaag tagcggaaga acacgtttgc ctggcccatc atcggtccga tgccacccat
      781 ctggaacatc agccactgca tcaccagcga gcggcccttg gcgtcggacg gcatcagccg
      841 gccggtcttc tcggcgagat agatcaggat ggcgccggat tcgaataccg cgaagttgtc
      901 ctcctcggta tcgacgatgg tcgggatgcg cgcgttcggg ttgagcttca cgtactccgg
      961 cttgcgctgg tcgccgcgcg tgatgtcgac cacgtgcacg gtgtacggca gcgccagctc
     1021 ttccaggcag acggaaatct tgtggccgtt gggcgtcggg gcggtgtaga gctcgatcat
     1081 gcggcgactc catcaattcc atgatttgcg ggaagcaacg cgtcgacctg cgcgcgtgca
     1141 gctgatgaca ggcgcgacct gtgtgtgagg caatcgcgcc agacgcgtgc ctggaaggtc
     1201 ttggccacgt ggcggaacgg gaagccgtcg atgacaccgt cgtacagcgc ctcgccgcgg
     1261 ttgaatgcgc gctcgttgaa acggcgctgg ccctgcacgt gcaggcgcgc gactgcctcc
     1321 gcgttctggc gcatcagcgc cacgtgcgtg cggcagattt ccgcgagcag cggtttcagt
     1381 ccggcatcga gctgaatatt ttccgcggcg ccgggtatgg gcgagggatc ggcgccgtgc
     1441 agcgcggtga gccaggcatg cacatgcggt gcccgctcgc gcatcaagcg gtcggcggaa
     1501 ggatcggaca ggttcatgcc gagctgtcca tagatcgccg cgtcggccag cgtgaacatc
     1561 ccgcccagca ggtagggccg gttcgccagc aacgtttcga ggatatcgag caggcgcagg
     1621 tgcgcgtgct cgagcaattc atgcgtcggc ggaaacccgg cgcgcgacgg cggctggcgc
     1681 gatgccggca ggccgttgat atggaaacct tccggcgcca cgctgaacag gtaggccagg
     1741 cggcgcgtct gccgcgcggc gaaccagcgc gcgccggcga actgcagcag gcccagcgag
     1801 gtgaagcgga attcgcgcgc cacgcgcgtg cccgcgtcgt tgtcgagcgc cgacactttc
     1861 cagcggttgt ggtggaccag gtacaagccc cattcgtcgg cgtaatcgtc gatcagccgc
     1921 gccacgaagc ttgccgcttc agcacctgga atcacgcgca gcgcgggttc gagccgcgca
     1981 tcgagccagc gcgcgatggc actggagtcg tacaggttct cgccctcggg gccgaacacg
     2041 aacggcacca gcgggaactc gtcgtcctcg gtcatggccg ggcaggtcag cggcagcatg
     2101 ccgcgcttga gccggtcgac gcgcagcgcg tagtgccagt tttccgtgaa tgaaccttcg
     2161 gccggcagga aacgcagcgg caggcgtgca tgccggaaca ggcggatcag cttgagggtg
     2221 aacggcgaca gctccgaacc ccagatgatc cagtcgccgc tcatggggct ttagaagcgc
     2281 gagccgacgc ggacgtcgct gctgttggcc ggcagcgaga tccaggtgcc atcgtgaccc
     2341 acggtgacgc cgcccttgta gataccggac acgccgcgca cgaacatctg ctccatcggc
     2401 cggtacggca gcgcgggcac caggtgatag aacagcaggt gcttcacgcc ggcatcgcgt
     2461 gcgatctccg cggcctgcac cggcgtggtg tgatagttga cgatgtctcg cgtgatctgc
     2521 gcgagattgg ccgcgccggc atcgaccgcc gcattggtca tgatcttcag taggtcgggg
     2581 ttcagcgcct cgtgtaccag caggtccgcg ccctgcgcaa agtgctgcag gttggccgac
     2641 ttcaccgtgt cgccggagat caccacgctg cggccctggt agtcgaagcg gtagccgacc
     2701 gcgggtacga tcggccggtg atccaccgcg aaggcggtga ccttgagccc gtcggtgtcg
     2761 agcaccaccg tgccttgtcc ttccgcgggt agttcaaacg gcatggcgat gccgccggcc
     2821 cccgagggcg ggatgatctt ttcgccgtga tgggccacgc ggtagtgatc gtcgtgcatg
     2881 taggcctggt tgaagccggc gaccacgtcc tccacgccgg tcgggccatg caccggcagc
     2941 ggcgaggtgc gcgaggcgcc ggcccagcgc tgcatcatca attcgcccag gccatcgatg
     3001 tggtcggaat ggaaatgggt gaggaacacg tcttcgatcc tcgccatcgg cacaccggcc
     3061 gggccgatgc gccgcacggc accggagccg acgtcgacca ccatcacgcg ggtgccggcg
     3121 atgaccagca cgcacggtcc ggagcgcgtc gggtcgggca gcggcgagcc agcgccgcac
     3181 agcaccacgt tgaggccgtc gttcatttcg cggaacgctt cgccggacag gttgcgcatg
     3241 atctgcttgc gcatggcggc ttcgaccagc cggtcgcgca tcaatgcgcc ggcggcggct
     3301 gccacgacga tgatcgccag cgctgtcagc aggatttttt tcagcatgtg tcgggattcg
     3361 gagtaggtaa ttcaacgacg ggccggatgc ccgcagcgcc tgcacgggac acccttgat
//
LOCUS       DASZZC010000622         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_374473,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000622 DASZZC010000000
VERSION     DASZZC010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374473"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VHE37_13275"
     CDS             <1..1016
                     /locus_tag="VHE37_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT36553.1"
                     /translation="SAVAVAPTTLAVITHDYLQERINHADPMLRHLLRVTTARHRQAM
                     RGASGATPAPACVEAPAADRDVALRRLRMEQDIERACEREEFELHYQPIVRVADTSVA
                     GFESLLRWNKPGQGRVSPGEFIPVAEESGLIRIIGRWIIDTAFAGIQRLEAVQRAAAP
                     STPPLFVTVNLSTRQMGDAALFDTLAASLAKHALAPQRVKLEITESMVMENMQTALEL
                     LHRCKALGTLLAVDDFGTGHSSLSYLHKFPMDTLKLDRSFLTDLPTSEASRKIVRAVV
                     NLAAELGMDSVFEGVEDAQQVQACREVGITYIQGYYYGKPMPLAAAAEFLSRHAAAPP
                     KAA"
     gene            1113..1631
                     /gene="infC"
                     /locus_tag="VHE37_13280"
     CDS             1113..1631
                     /gene="infC"
                     /locus_tag="VHE37_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HVT36554.1"
                     /translation="MAAEKEDRRNHEITAPRVRVIGADGSQAGIMMTRDAVAKAEELG
                     LDLVEVSPNADPPVCKIMDYGKYLYAKDKAAHAAKRKQKQIQVKEIKFRPSTEEADYQ
                     TKLRAIQRFFEEGDKVKITVRFRGRELAHSEIGMQLMDRLKAELEPIATIEQFPKMEG
                     KQSVMVMSPKRR"
     gene            1728..1925
                     /gene="rpmI"
                     /locus_tag="VHE37_13285"
     CDS             1728..1925
                     /gene="rpmI"
                     /locus_tag="VHE37_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HVT36555.1"
                     /translation="MPKMKTVKSAAKRFAKTGKGGFKRSHSHKRHILTKKGGKRIRQL
                     RGEAQVHASDVREVRIMLPYA"
     gene            1938..>2289
                     /gene="rplT"
                     /locus_tag="VHE37_13290"
     CDS             1938..>2289
                     /gene="rplT"
                     /locus_tag="VHE37_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008487290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVT36556.1"
                     /translation="MARVKRGVTAKARHKKMLKKAKGYYGARSRTYRSAKQQVIKSGQ
                     YAFRDRRVKKREFRALWIARINAAVRELGMTYSVFIAGLNKASIDIDRKVLADLAVHD
                     KAAFGKIAEQAKASL"
BASE COUNT          459 a          749 c          761 g          320 t
ORIGIN      
        1 cgtcggcggt ggcggtggcg cccaccacgc tggcggtgat cacgcacgat tacctgcagg
       61 agcgcatcaa ccacgcggac cccatgctgc gtcacctgct gcgcgtgacc acggcgcgcc
      121 accgccaggc catgcgcggc gccagcgggg cgacgccggc gcccgcgtgc gtggaggctc
      181 ccgcggcgga ccgcgacgtg gcgctcaggc gcctgcgcat ggagcaggac atcgagcgcg
      241 cctgcgaacg cgaggaattc gagctgcact atcaacccat cgtgcgcgtg gccgacacca
      301 gcgtggccgg cttcgaatcg ttgctgcgct ggaacaagcc ggggcagggc cgcgtgtcgc
      361 cgggcgagtt catcccggtg gcggaggaat ccgggctgat ccgcatcatc ggccgctgga
      421 tcatcgacac cgcgttcgcc ggcatccagc ggctggaagc ggtgcagcgc gcagcggcgc
      481 cttcaacgcc gccgctgttc gtcacggtga acctgtcgac ccggcagatg ggtgacgcgg
      541 cgctgttcga cacgctggcg gcgtcgctgg ccaagcatgc gctggcgccg cagcgggtca
      601 agctggagat caccgagagc atggtgatgg agaacatgca gaccgcgctg gagctgctgc
      661 accgctgcaa ggcgctgggc acgctgctgg ctgtcgacga tttcggcacc ggccacagct
      721 cgctgtccta cctgcacaag ttcccgatgg acacgctcaa gctggaccgc tcgttcctca
      781 ccgacctgcc gaccagcgag gccagccgca agatcgtgcg tgcggtggtc aacctggcgg
      841 ccgaactggg catggacagc gtgttcgagg gcgtggagga cgcgcagcag gtgcaggcct
      901 gccgcgaggt gggcatcacc tacatccagg gctactacta cggcaagccg atgccgctgg
      961 ctgccgccgc cgaattcctc agcaggcatg ccgccgcgcc gcccaaggcc gcttgagtcg
     1021 ccccggcagg ctctatacaa ttcgccccct ttcctcgccg cccggcacga tgccgcgtgg
     1081 cgaaaatcca ggcctgacgc gggagatcac gcatcgcagc tgaaaaagaa gaccggcgca
     1141 accacgagat aacggcgccg cgcgtgcgcg tgatcggagc ggatgggtcg caagccggca
     1201 tcatgatgac ccgtgatgcc gtggccaagg ccgaggaatt ggggctggac ctcgtcgagg
     1261 tttcgcccaa cgccgatccg cccgtgtgca agatcatgga ttacggcaag tacctttatg
     1321 ccaaggacaa ggccgcccat gccgccaagc gcaagcagaa gcagatccag gtcaaggaaa
     1381 taaaattccg gcccagcacc gaggaagcgg attaccagac caagctgcgc gccatccagc
     1441 ggttcttcga ggaaggcgac aaggtcaaga tcacggtgcg cttccgcggc cgcgaactgg
     1501 cccactccga gatcggcatg cagttgatgg accggctcaa ggccgagctg gaacccattg
     1561 ccaccatcga gcagtttccg aagatggaag gcaaacagtc ggtgatggtg atgtcaccga
     1621 agcggcgcta gtacgccgca gatttcaagt tccatccgca gggatggacc cggcaagctc
     1681 ccgcgggcgg gatgcgacac cggccaccta gcagtacgga ggtacccatg cccaagatga
     1741 aaaccgtgaa gagcgcggcc aagcgctttg ccaagaccgg caaaggcggc ttcaagcgca
     1801 gccactcgca caagcgccac atcctgacca agaagggtgg caagcgcatc cgccagttgc
     1861 gcggcgaagc ccaggtccac gccagcgacg tgcgcgaagt gcgcatcatg ctgccgtacg
     1921 cataagggag aacgaccatg gcacgagtca aacgcggcgt cacggcgaaa gcccgccaca
     1981 agaaaatgct gaagaaggcg aagggctact acggtgcccg ttcgcgcacc taccgctccg
     2041 ccaagcagca ggtgatcaag tccggccagt acgccttccg cgaccgccgg gtgaagaagc
     2101 gcgagttccg cgcgctgtgg atcgcgcgga tcaacgcggc ggtgcgcgag ctcgggatga
     2161 cgtacagcgt gttcattgcc ggcctcaaca aggcgtcgat cgacatcgac cgaaaggtgc
     2221 tcgccgacct cgcggtgcat gacaaggcgg cgttcggcaa gatcgccgag caggccaagg
     2281 cctcgttgg
//
LOCUS       DASZZC010000623         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1266792,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000623 DASZZC010000000
VERSION     DASZZC010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266792"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2260
                     /locus_tag="VHE37_13295"
     CDS             <1..2260
                     /locus_tag="VHE37_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVT36557.1"
                     /translation="HGSFSPASTQIPAAACKTWRDLRHADDPDATRTISKSGFHQSPA
                     TLNENCPQPVLPGVAVWDVGSVKPFAAYTAAGVNQNNGTITLNTPQRPLLRNSKQKRG
                     AATMLARAGKPATVALGPTALLKNALAAAGFGLPASMSNWMAVTADRTADGHPIGIMG
                     PQMSYFQPNLPWEFAVHSTGGTPLDFDGRGMGFGTLPFILIGRGTDYAWSATSGDSDV
                     IDTRVSKLCNMDGSTPSRDDANGDGFPDADGYLYDLGDGNGPQCRRFYKRTDTWTATP
                     TVASLGSGGGPQPAAIQRNILRTHYGPVFATATVNGAPVVISTQRSSFYGELDSSGPI
                     AFASTPLVHDAASFQHLFNGITGTFNWLYVDKKDVGYIASGLFPLRDPNMSPELPAWG
                     DGRYEWASDQHLPADFFSKYGGDTPFPSRVVPVQTGGDQLHGGYTEWQNFLPFSLHPQ
                     VVNPPEGFIESWNNSPAPGWWASDSRANWGVVHRTDTEVPLFNAFIASGRKFDFANVM
                     EIMGDAGYADLRAQKLLPLLLQIMAQGTLTADEQSIVPLMQQWLDAGSLQWINGQKGQ
                     GAWRRDRDGDGTYDSRAAVVLMDAWYLHLMDTVTPDLKALAPNADVPGLVECVSNILL
                     CKYDAPRAQGSAFEYGWYQVMIRELQMVLATPGHHDYQTLKCAGTGVLADCRKAVLDA
                     LDAAITDLGGVSNQASWDGTQLPNAKNKSNTAVEKYDQIEFTDFSSLPQLTMPWSNRP
                     TYQQVVEVKSGR"
     gene            2393..>3046
                     /locus_tag="VHE37_13300"
     CDS             2393..>3046
                     /locus_tag="VHE37_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVT36558.1"
                     /translation="MSSLLPLHAAHPALAQAYPVAGLLRGPTRVQPLAGTRDCWLKRD
                     DLTAADYGGNKIRKLDFLLADAQRRGKKTLVAFGYAGSNFVAATAWHGRKLGLRTIGC
                     LLPQANAGYVADNLSVSLAAGAELIARPKKFPLVRAAAAASLKAWRRDSAWPLWIPPG
                     GSNALGALGYVNAAYELKAQIDARAVPAPQAIYLPISSMGSVAGLVAGLALAGLDVQI
                     "
BASE COUNT          544 a         1062 c          975 g          465 t
ORIGIN      
        1 ccacggctcg ttctcgccgg cctcgacgca gatcccggcg gccgcctgca agacctggcg
       61 cgacctgcgc catgccgacg atcccgacgc cacgcgcacc atcagcaaga gcggcttcca
      121 ccagtcgccg gccacgctca acgagaactg cccgcagccg gtgctgcccg gcgtggcggt
      181 gtgggacgtg ggcagcgtca agccctttgc cgcctacacc gccgcaggcg tcaaccagaa
      241 caacggcacc atcacgctga acacgccgca gcggccgctg ctacggaatt cgaagcagaa
      301 gagaggcgcg gcgaccatgc tggcgcgcgc cggcaagccg gccacggtgg cgctcggccc
      361 gaccgcgctg ctgaagaacg cgctggccgc ggcgggattc ggtttgccgg cgtcgatgtc
      421 caactggatg gcggtgaccg ccgaccgcac ggccgacggc cacccgatcg gcatcatggg
      481 tccgcagatg agctacttcc agccgaacct gccgtgggag ttcgcggtgc attccaccgg
      541 cggcacgccg ctggatttcg acggccgcgg catgggcttc ggcacgctgc ccttcatcct
      601 gatcggccgc ggcaccgatt acgcgtggag cgccacctcc ggcgacagcg acgtgatcga
      661 cacgcgcgtg agcaagctgt gcaacatgga tggcagcacg cccagccgcg acgatgccaa
      721 cggcgacggc ttccccgatg ccgacggtta tctctacgat ctgggcgacg gcaacggccc
      781 gcaatgccgc cgcttctaca agcgcaccga cacctggacc gcgacgccca ccgtggcctc
      841 gctcggctcc ggcggcgggc cgcagccggc agcgatccag cgcaatatcc tgcgcacgca
      901 ctacggcccg gtgttcgcca ccgccaccgt caacggcgcg ccggtcgtga tctccaccca
      961 gcgcagcagt ttctacggag agctcgactc gtccggaccc atcgcgttcg cctcgacacc
     1021 gctggtgcac gatgcggcca gcttccagca cctgttcaac ggcatcaccg gcaccttcaa
     1081 ctggctctac gtcgacaaga aagatgtcgg ttacatcgcc agcggcctgt tcccgctgcg
     1141 cgatccgaac atgtcgccgg aactgccggc ctggggcgat ggccgctacg agtgggccag
     1201 cgaccagcac ctgcccgccg atttcttcag caagtacggc ggcgacacgc cgttcccctc
     1261 gcgcgtggta ccggtgcaga ccggcggcga ccagctgcat ggcggctaca ccgagtggca
     1321 gaacttcctg ccgttctcgc tgcacccgca ggtggtcaat ccgccggaag gcttcatcga
     1381 aagctggaac aactcgccgg cgccgggctg gtgggccagc gacagccgcg ccaactgggg
     1441 cgtggtgcat cgcaccgaca ccgaggtgcc gctgttcaat gccttcatcg ccagcggacg
     1501 caagttcgat ttcgccaacg tgatggagat catgggcgac gccggctacg ccgacctgcg
     1561 cgcgcagaag ctgctgccgc tgctgctgca gatcatggcg cagggcacgc tcaccgccga
     1621 tgaacaaagc atcgtgccgt tgatgcagca atggctcgac gccggctcgc tgcagtggat
     1681 caacggccag aaaggacaag gtgcatggcg gcgcgaccgc gatggcgacg gcacctacga
     1741 cagccgcgcc gcggtggtgc tgatggatgc ctggtacctg cacctgatgg acaccgtcac
     1801 acccgacctc aaggcgctgg cgcccaacgc cgacgtgccg ggcctggtcg agtgcgtatc
     1861 caacatcctg ctgtgcaagt acgacgcgcc acgcgcgcag ggcagcgcat tcgagtacgg
     1921 ctggtaccag gtgatgatcc gcgaactgca gatggtgctg gccacgccgg gccaccacga
     1981 ctaccagacg ctcaagtgcg ccggcaccgg cgtgctggcc gattgccgca aggccgtgct
     2041 cgatgcgctc gatgcagcga tcacggacct cggcggcgtc agcaaccagg ccagctggga
     2101 cggcacgcaa ttgcccaatg cgaaaaacaa gagcaacacc gcggtggaaa aatatgacca
     2161 gatcgagttc accgattttt ccagcctgcc gcagctgacc atgccgtgga gcaaccgccc
     2221 gacctaccag caggtggtgg aagtgaaaag cgggcgttga ggcgcgcatt gcttgatcca
     2281 ccgcacgacc attttgcttg ttattaatta tcgtctaaat gttaaatcag catatttttt
     2341 gtaacacatc aaatcggggt gcggtgaagc tggaaatgcc gccggcgcgg acatgagctc
     2401 gttgctaccg ctgcacgccg cgcatcccgc gctggcgcag gcgtatccag tcgccggcct
     2461 gctgcgcggc cccacgcgcg tgcagccgct ggccggcacg cgcgactgct ggcttaagcg
     2521 cgacgatctc accgccgccg actacggcgg caacaagatc cgcaagctcg acttcctgct
     2581 ggccgatgcg cagcggcgcg ggaagaaaac gctggtggcc ttcggctatg ccggttccaa
     2641 tttcgtcgcg gcgaccgcgt ggcacgggcg caagctcggc ctgcgcacca tcggctgcct
     2701 gctgccgcag gccaacgccg gctacgtggc cgacaatctt tccgtctccc tcgccgccgg
     2761 cgcggaactg atcgcgcggc cgaaaaaatt cccgctggtg cgcgcggcgg ccgctgcatc
     2821 gcttaaagca tggcgacgcg acagcgcctg gccgctgtgg attccaccgg gtggatcgaa
     2881 tgcgctcggc gcgctgggtt acgtcaatgc ggcgtatgaa ctgaaagcgc agatcgacgc
     2941 gcgcgcggtg ccggcaccgc aggccatcta cctgccgatc tccagcatgg gcagcgtggc
     3001 cgggctggtc gcgggcctcg cgctcgccgg gctcgatgtg cagatc
//
LOCUS       DASZZC010000624         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1146271,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000624 DASZZC010000000
VERSION     DASZZC010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146271"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1757
                     /locus_tag="VHE37_13305"
     CDS             141..1757
                     /locus_tag="VHE37_13305"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HVT36559.1"
                     /translation="MRKFILLGLPLVLAACGGGNALGSANPQVPTQAGVSGTPPPQGQ
                     QPPPPPPPPPQQQPPPPPPAADLDQQLRAIIQAQGLTGDPSRGRDVPDISSPLAQLGM
                     RLFFSRSLSGDADVACASCHLPTLAGADQLSMPVGVAADDPSLIGPGRSNAADQPIVP
                     RNAPTTFNAVLWDHFMFDDGRIESLRPQPGANGAAGQIRTPDVPFAQPDPEAGSTLPA
                     AQSRFPVTVDAEMRGAMFEAGNGNDDVRNHLAARIGNYGAGKGELKRDRWLPLFQAAF
                     NVSTGARSVITYPNIAEALATYERSQLFVDNPWRDYVGGNNAAIPDAAKRGAIAFLTP
                     GNRGGAGCSRCHRGDAFTDEQFHVIGVPQIGVGKGDGAHGDDDFGRGRESGDANDRYA
                     YRTPSLLNVALTAPYDHDGVFATLDRVVQHYRNPAQSATGYVNGRQWCALRQFQQLPL
                     AQCQALYPDAAANTQAALNQLNAQRGTPRGLPPTNISNQQAADIVAFLQTLSSRCAAD
                     RNCAARWTPPRDGGPDGQQLAPVDQHGNPL"
     gene            1781..>2261
                     /locus_tag="VHE37_13310"
     CDS             1781..>2261
                     /locus_tag="VHE37_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36560.1"
                     /translation="MQKIFFAAALVLAALNAGAQSGPSNQGGMPSMQGQGSGPSMMGG
                     EHHGPPPSVERAAIHKLLAQELSRASGRAPSEITQMLATSPPPEVARQLGLQPDAVKS
                     AMQNAHAQLIQKALAAGLIDQADVPAIQQEDQRRMQQMPMMGEGTRSGMPPQPQGQNH
                     "
BASE COUNT          381 a          821 c          757 g          302 t
ORIGIN      
        1 cagcttcgag cgcatggcgg catcgtaatg caggcgcgcg cgcagccggc gcatgcgcag
       61 cgcttccacc gaatttgccc atcatttccc cacaatcgcg acctagcctc gctgccatgg
      121 acattcatgg gagacgggac atgcgcaagt tcatcctgct ggggctgccg ctcgtgctgg
      181 cggcatgcgg cggcggcaac gcactgggca gcgccaaccc gcaggtgccc acgcaggcgg
      241 gagtgagcgg gacgccgccg ccgcagggcc agcaaccgcc gccaccccca ccaccgcctc
      301 cgcagcagca acctccgcca ccgccgccgg ccgccgatct cgatcagcag ctgcgcgcca
      361 tcatccaggc gcaggggctg accggcgatc cttcgcgcgg ccgcgacgtg ccggacattt
      421 catcgccgct ggcgcaactg ggcatgcgcc tgttcttctc gcgcagcctg tcgggcgatg
      481 ccgacgtggc ctgcgccagc tgccatctgc cgacgctggc gggcgcggac cagttgtcga
      541 tgccggtcgg cgtggcggcg gatgacccgt cgctgatcgg cccgggccgc agcaacgcag
      601 ccgaccagcc catcgttccg cgcaacgcgc cgaccacgtt caatgcggtg ctgtgggatc
      661 acttcatgtt cgatgacggg cgcatcgaat cgctgcgacc gcagcccggc gccaacggcg
      721 cagcggggca gatccgcacg ccggacgtgc cgttcgcaca acccgatccg gaggccggca
      781 gcacgctgcc cgcggcgcag tcgcgcttcc cggtcacggt cgacgccgaa atgcgcggtg
      841 ccatgttcga agccggcaac ggcaatgacg acgtgcgcaa tcacctggcg gcgcgcatcg
      901 gcaactacgg cgcgggcaag ggtgagctca agcgcgaccg gtggctgccg ctgttccagg
      961 cggcgttcaa tgtgtctacc ggcgcgcgca gcgtgatcac ctatccgaac atcgccgagg
     1021 cgctggccac ctacgagcgc tcgcagctgt tcgtcgacaa cccgtggcgc gattacgtgg
     1081 gcggcaacaa cgcggccata cccgacgcgg ccaagcgcgg cgccatcgcc ttcctcacgc
     1141 ccggcaaccg cggcggcgcc ggctgctcgc gctgccatcg cggcgacgcc ttcaccgatg
     1201 agcagttcca cgtgatcggc gtgccgcaga tcggcgtggg caagggtgat ggcgcgcatg
     1261 gcgacgatga tttcggccgc ggccgcgaga gcggcgacgc caatgaccgt tacgcctatc
     1321 gcacgccgtc gctgctcaac gtggcgctca ccgcgcccta cgatcatgac ggcgtcttcg
     1381 ccacgctgga ccgcgtggtg cagcactacc gcaatcccgc gcagtcggcg accggctacg
     1441 tcaacggcag gcagtggtgc gcgctgcggc agttccagca gttgccactc gcgcaatgcc
     1501 aggcgcttta tcccgatgca gcggccaaca cgcaggcggc gctcaaccag ctcaacgcgc
     1561 agcgcggcac gccgcgcggc ctgccgccca ccaacatcag caaccagcag gcggccgaca
     1621 tcgtcgcctt cctgcagacg ctgagcagcc gctgcgccgc cgatcgcaat tgcgcggcgc
     1681 gctggacgcc gccacgcgac ggcggaccgg acggccagca gctggcgccg gtcgatcagc
     1741 acggcaatcc tctttaagct cgacgaacca ggagatgaac atgcagaaga ttttttttgc
     1801 cgcagcactt gtgttggcgg cgctgaacgc cggcgcgcag tccggtccgt cgaaccaggg
     1861 cggcatgccc tcgatgcaag ggcagggatc gggaccgtcg atgatgggtg gcgagcatca
     1921 cggcccgccg ccgtcggtgg agcgcgccgc catccacaag ctgctggcgc aggaactgtc
     1981 ccgcgccagc ggccgggcgc catcggagat cacccagatg ctggcgacct cgccgccgcc
     2041 ggaggtggcg cgccagctcg gcctgcagcc cgacgcggtg aaaagcgcca tgcagaacgc
     2101 gcacgcgcag ctgatccaga aagcgcttgc cgcggggctg atcgaccagg ccgacgtgcc
     2161 ggcgatccag caggaagacc agcgccgcat gcagcagatg ccgatgatgg gcgaaggtac
     2221 gcgcagcggc atgccgccgc agccgcaagg tcagaaccat g
//
LOCUS       DASZZC010000625         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_111139,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000625 DASZZC010000000
VERSION     DASZZC010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111139"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1440)
                     /locus_tag="VHE37_13315"
     CDS             complement(91..1440)
                     /locus_tag="VHE37_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36561.1"
                     /translation="MRMPAALLLMVSIAAAAQAPDEAGDGADKLQAFLAQCRARYAEV
                     DARIDASGRRDAGYFRVPGFPYLRSDRLMASFGPELGNPDAFDDWTLQLRDNDAYSRE
                     VEFSNMGLSTAERAQLLVDLRLCAVWLTNTELADAATLKKLQAAMQLPRAAAHASAHE
                     DAAWRRRVRDDFSAAPLDLVHDQLWLPAPLPDPREVPRSFDHALHDHLGRAGLLMGEW
                     PVLAARYAPALSIETRGEFDRPGTPRWQAGALSIDAQQPAVYYLPSYARVQGRMLVQI
                     SYFIWFAGRGAGDVRTDGLIWRVTLDEDGEPVMADSVHTGGGDQLWFPLAGWLVRADA
                     RDGALVPQAQVPREHAVHLRSGAHAVTRLLARADEAAAQRYTLHPYEDLLTLPLPGGG
                     TRSLYDAQGRVAGSSDLYQWSWQTTSPRATRYFDDPDLLESLFVPLPRSAVSTAAQR"
     gene            complement(1440..>3527)
                     /locus_tag="VHE37_13320"
     CDS             complement(1440..>3527)
                     /locus_tag="VHE37_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVT36562.1"
                     /translation="DQILSVTTTKARYSEATPQGIDMRPLMADVPPRTPEEIAAFREH
                     AQHAPNVKVFLVSQDERSALVMATFIEQRLGYGELFRYLQDLAARTRDAHHQVYLAGQ
                     PMLIGWVYQYERQMLLIFGVTLAALLLALTLYMRNLVGVLTPILCSATAAIWAFGFVG
                     ALHVSIEPLLMVVPLLLTARSFSHSVQFTERYYEVYARVHDRRKAAEITMALMMAPSV
                     LGILTDILGIVVVIAAPIPAMVRHAIFCGMWAVWLIPTGVVLISLLLATLPAPRNLGQ
                     LLGAGRTQARLQRVLRAVASLTHGPRARVSSALVIAGSLAAVTIAAQIRIGNPVEGSS
                     LLWDRSEFNTAVRAINANFPGVNTLEIVLEAKNADDANWTAQQVDTVMTLQRLQYLME
                     SGNAPPRATLSFADYLAEANRLFNGGNPKWLPLDPRPRAISAAATGAMMGSSAKNFGH
                     VMSDDAQHATVSLWYRDNRQETVDAALAAATAAVNAVGVEHDAFRVRLGTGLIALQEA
                     VNRVIARYQWLVLAAVNAMIFVMCALAYRSWMAGALLLLPVNLANQAMIAAMHLLGVG
                     LDVNSMIVAAIGIGVGIDYGIYLLSRICEEHRSHAGDWGAALDAALATTGKAILYTAS
                     IMTLGILPWYLMSDLKFVADMGLLLMAIMVINMVLALLVLPLSVWWLRPAFVARGQRL
                     LGEGIDARLLGVS"
BASE COUNT          583 a         1206 c         1218 g          520 t
ORIGIN      
        1 gcgtgttatt aattattatt caatataact tttagacgtt aatttataac agcctaaacc
       61 cgggtgcggt gacggtatgc agcgcggatt tcagcgctgt gccgccgtgg aaaccgcgct
      121 gcgcggcagc ggcacgaaca gcgactccag caggtcggga tcgtcgaagt agcgcgtcgc
      181 ccgcggcgac gtggtctgcc agctccattg ataaagatcg ctgctgccgg cgacgcggcc
      241 ctgcgcgtcg tacaggctgc gcgtgccgcc gccgggcagc ggcagcgtga gcaggtcctc
      301 gtacgggtgc agcgtgtagc gctgtgctgc cgcctcgtcg gcgcgtgcca gcaggcgcgt
      361 caccgcgtgc gcgcccgagc gcaggtgcac ggcgtgttcg cgcggcacct gcgcctgcgg
      421 taccagcgcg ccgtcgcgcg catccgcgcg cacgagccag ccggcgagcg gaaaccacag
      481 ctggtcaccg ccaccggtgt gcacgctgtc ggccatcacc ggttcgccgt cctcgtcgag
      541 cgtgacgcgc cagatcagcc cgtcggtgcg cacgtcgccg gcgccgcgtc cggcgaacca
      601 gatgaagtag ctgatctgca ccagcatgcg gccctgcacg cgtgcgtacg acggcaggta
      661 atagaccgcc ggctgctgcg cgtcgatgga cagcgcgcca gcctgccagc gcggcgtgcc
      721 ggggcggtcg aactcgccgc gcgtctcgat cgacagcgcc ggtgcgtagc gcgcggccag
      781 caccggccac tcgcccatca gcaggccggc gcggcccagg tgatcgtgca gcgcgtggtc
      841 gaacgagcgc ggcacctcgc gcggatcggg cagcggcgcg ggcagccata gctggtcgtg
      901 cacgagatcg agcggggctg cagaaaaatc gtcgcgcacg cgccgacgcc atgctgcatc
      961 ttcatgcgcg gacgcatgcg ccgccgcgcg cggcagctgc atcgcggcct gcagcttctt
     1021 cagcgtggcg gcgtcggcca gctcggtgtt ggtcagccac accgcgcaca ggcgcaggtc
     1081 caccagcagc tgcgcgcgct cggccgtcga caggcccatg ttggaaaatt ccacttcgcg
     1141 cgaatacgca tcgttgtcgc gcagctgcag cgtccagtcg tcgaaggcgt ccgggttgcc
     1201 aagctccggc ccgaacgagg ccatcaggcg gtcgctgcgc aggtagggaa agccaggcac
     1261 gcggaaatag ccggcatcgc gccggccgga cgcatcgatg cgcgcgtcga cctcggcata
     1321 gcgcgcgcgg cattgcgcga ggaaggcctg cagcttgtcg gcgccgtcgc cggcttcgtc
     1381 cggcgcctgc gccgcggccg caatggaaac catcagcagc aacgcggcgg gcatgcgcat
     1441 cacgaaactc cgagcaggcg tgcgtcgatg ccttcgccga gcaggcgctg cccgcgcgcc
     1501 acgaacgccg ggcgcagcca ccacaccgag agcggcagca ccagcagcgc cagcaccatg
     1561 ttgatgacca tgatcgccat cagcagcagc cccatgtcgg cgacgaactt gaggtccgac
     1621 atcaggtacc agggcaggat gccgagcgtc atgatgctcg ccgtgtagag gatggccttg
     1681 ccggtggtgg ccagcgcagc gtcgagcgct gcgccccagt cacccgcatg tgaacgatgc
     1741 tcctcgcaga tgcgcgacag caggtagatg ccgtagtcga tgccgacgcc aatgccaatc
     1801 gccgccacga tcatcgagtt cacgtccagc ccgacgccga gcaggtgcat ggccgcgatc
     1861 atcgcctggt tggccaggtt caccggcagc agcagcagcg cgccggccat ccacgagcgg
     1921 taggccagtg cgcacatgac gaagatcatc gcgttcaccg cggccagcac cagccattgg
     1981 tagcgcgcga tgacgcggtt caccgcctcc tgcagcgcga tcagcccggt gcccagccgc
     2041 acgcggaagg catcgtgctc gacgccgacg gcgttcaccg cggcagtcgc cgccgcgagc
     2101 gccgcatcca ccgtttcctg ccggttgtcg cggtaccaca gcgagacggt ggcgtgctgc
     2161 gcgtcgtcgc tcatcacgtg gccgaagttc ttggcgctgg agcccatcat ggcgccggtg
     2221 gcggcggcgc tgatggcgcg cggccgcgga tcgagcggca gccatttcgg attgccgccg
     2281 ttgaacaggc gattggcctc cgccaagtaa tcggcgaacg acagcgtggc gcgcggcggt
     2341 gcatttccgc tctccatcaa atactgcagg cgctgcagcg tcatcaccgt gtccacctgt
     2401 tgcgcggtcc agttggcgtc gtccgcgttc ttcgcttcca gcacgatctc cagcgtgttc
     2461 acgccgggaa agttggcgtt gatggcgcgc accgcggtgt tgaattcgga gcggtcccac
     2521 agcaggctgc tgccttccac cgggttgccg atgcggatct gtgcggcaat ggtgaccgcg
     2581 gcaagcgagc cggcaatcac cagcgcgctg ctcacgcgcg cgcgcggccc gtgggtgagc
     2641 gaggccaccg cgcgcagcac gcgctgcagg cgcgcctgcg tgcggccggc gccgagcaac
     2701 tggccgaggt tgcgcggcgc cggcagcgtg gccagcagca gcgagatcag caccacgccg
     2761 gtggggatca gccacaccgc ccacatgccg cagaaaatgg cgtggcgcac catggccggg
     2821 atcggcgcgg caatcaccac cacgatgccg aggatgtcgg tgaggatacc cagcacgctg
     2881 ggcgccatca tcagcgccat ggtgatctcg gcggccttcc tgcggtcatg cacacgcgcg
     2941 tagacctcgt agtagcgctc ggtgaactgc acgctgtgcg agaacgagcg cgcggtgagc
     3001 agcagcggca ccaccatcag cagcggctcg atcgacacgt gcagcgcgcc gacgaagccg
     3061 aacgcccaga tggcggctgt cgcgctgcag aggatcggcg tcagcacgcc gaccaggttg
     3121 cgcatatata aggtcagcgc cagcagcagc gccgccaggg tgacgccgaa gatcagcagc
     3181 atctgccgct cgtactggta gacccagccg atcagcatgg gctggccggc caggtagacc
     3241 tggtggtgcg cgtcgcgcgt gcgcgcggcc aggtcctgca ggtagcggaa cagctcgccg
     3301 taacccaggc gctgctcgat gaaggtggcc atcaccagcg ccgagcgttc gtcctgcgag
     3361 accaggaaca ccttcacgtt gggcgcatgc tgtgcatgct cgcggaaggc ggcgatctcc
     3421 tccggcgtgc gcggcggcac gtcggccatc agcgggcgca tgtcgatgcc ctgcggcgtg
     3481 gcctcgctgt agcgcgcctt ggtggtggtg accgacagga tctggtc
//
LOCUS       DASZZC010000626         4877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_499874,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000626 DASZZC010000000
VERSION     DASZZC010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499874"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VHE37_13325"
     CDS             <1..270
                     /locus_tag="VHE37_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36563.1"
                     /translation="GMDHYDANFAKEVIPKLAGASPAQRMGEEAEVSAAIVFLLSEGA
                     SYISGTCINIDGASSRNSRIFPMQAHGRSKPFRGFHRAVKPRAVS"
     gene            381..2978
                     /gene="acnD"
                     /locus_tag="VHE37_13330"
     CDS             381..2978
                     /gene="acnD"
                     /locus_tag="VHE37_13330"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HVT36564.1"
                     /translation="MNSAFRKKLPGTSLDFFDAREAVDAIQPGAWARLPYTARVHAEN
                     LVRMGEPGKINEYLGQLIERKRDLDFPWFPVRVVCHDILGQTALVDLAGLRDAIADRG
                     GDPAKVNPVVPVQLIVDHSLAVEHAGFEKDAFAKNRAIEDRRNDDRFHFINWTKAAFK
                     NVDVIPPGNGIMHQINLERMSPVIYVKDGVAYPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAENVMLGRASWMRLPDIVGVELSGKPREGITATDVVLALTEFLRKEKVVGAYLEFR
                     GEGAAALTLGDRATISNMAPEYGATAAMFFIDQNTIDYLRLTGRSDEQVKLVETYARH
                     TGLWADALKTAVYERTLQFDLSTVVRNMAGPSNPHKRLPTSALAERGIAANWELPADG
                     KMPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANARGLTRKPWVKSSLAPGSKVVES
                     YLKEAKLLPELEQLGFGIVAFACTTCNGMSGALDPKIQQEIVERDLYATAVLSGNRNF
                     DGRIHPYAKQAFLASPPLVVAYAIAGTVRFDIEKDVLGTDKNGQPVRLKDIWPTDAEI
                     DAVVASSVNPTQFRAVYEPMFRKQADDGAKIKPLYDWRAQSTYIRRPPYWEGALAGER
                     TLRGLRPLAVLPDNITTDHLSPSNAILMDSAAGEYLHKMGLPEEDFNSYATHRGDHLT
                     AQRATFANPQLVNEMAVVDGKVKKGSLARIEPEGKVTRMWEAIETYMQRKQPLIIIAG
                     ADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGCLPLEFKPGTTRLTLKI
                     DGTETFDVIGARKPRATLTLVIHRKNGERVEVPVTCRLDSDEEVSIYEAGGVLQRFAQ
                     DFLESNKAA"
     gene            3394..3822
                     /locus_tag="VHE37_13335"
     CDS             3394..3822
                     /locus_tag="VHE37_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVT36565.1"
                     /translation="MFGKQQAASTPVDFGKQKGLYILYDHHTVVYVGRAIDRPLGRRL
                     YEHTIDRLASRWNRFSWFGFHEVTDDGRLQDVPVKTSLAALVATLEALLIESLEPPQN
                     RKRGDDFSVNEYIQDGDPELKERELQNTLRAIEQKLRGRN"
     gene            3841..>4877
                     /gene="prpF"
                     /locus_tag="VHE37_13340"
     CDS             3841..>4877
                     /gene="prpF"
                     /locus_tag="VHE37_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HVT36566.1"
                     /translation="MTGAPQIKIPATYMRGGTSKGVFFRLQDLPAPVQQPGPARDKFL
                     MRVIGSPDPYAAHIDGMGGATSSTSKTVIIGKSAQPGHDVDYLYGQVSIDKAFVDWSG
                     NCGNLSTAVGPFAVANGFIEKSRLPKDGVFAVKVWQANIGKTIICHVPIKNGEVQETG
                     DFELDGVTFPAAEIVLEFLDPSDDGDAGGSMFPTGNLVDDLDVPGIGRLKATMITAGI
                     PTVFVNAADIGYTGTELREAINTKPEALAKFEAIRVAGALRMGLIKQPEEAATRQHTP
                     KVAFVAPPKDYVSSSGKTIRASEIDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTL
                     VNLAAGGGQREA"
BASE COUNT          971 a         1545 c         1561 g          800 t
ORIGIN      
        1 ggcatggatc actacgacgc caatttcgcc aaggaagtca tccccaagct tgcgggcgcc
       61 tcgcccgcgc agcgcatggg cgaagaggcg gaagtgtccg ccgccatcgt cttcctgctg
      121 tcggaaggcg cgagctacat cagcggcacc tgcatcaaca tcgacggggc ctcgtcgcgc
      181 aacagccgca ttttcccgat gcaggcgcac ggcaggtcga agccgttccg tgggttccac
      241 cgcgcggtga agcccagggc cgtgagctga gcggggcggt gcccagaccc cgtccgaacg
      301 ggcgggtctg ggggcgggcg cgacgcgata aaatcgcgct cttgaagcat gaacgtccgc
      361 cccagcctga ctgcctcgcc atgaactcag cctttcgcaa gaaacttccc ggcacttccc
      421 tcgacttctt cgacgctcgc gaggcggtcg acgcgatcca gcccggtgcc tgggccaggc
      481 tgccttacac cgcccgcgtg catgccgaga acctggtgcg catgggcgag cccggcaaga
      541 tcaatgagta tctcggccag ctgatcgagc gcaagcgcga cctcgatttc ccctggttcc
      601 cggtgcgcgt ggtgtgccac gacatcctgg ggcaaacggc gctggtcgac ctggccggcc
      661 tgcgcgacgc catcgccgac agggggggcg atccggcgaa agtaaacccg gtcgtgccgg
      721 tgcagctgat cgtcgatcac tcgctggccg tggagcacgc cggttttgag aaggatgcgt
      781 ttgccaagaa ccgcgcgatc gaagaccgcc gcaacgacga ccgcttccat ttcatcaact
      841 ggaccaaggc ggcgttcaag aacgtggacg tgattccgcc gggcaacggc atcatgcacc
      901 agatcaacct ggagcggatg tcgccggtga tctacgtgaa ggacggcgtc gcctaccccg
      961 atacctgcgt gggcaccgat tcgcacacgc cgcacgtgga cgcgctgggc gtgatcgcga
     1021 tcggcgtcgg cggcctggag gccgagaacg tgatgctggg ccgcgcctcg tggatgcgcc
     1081 tgccggacat cgtcggcgtg gaactctcgg gcaagccgcg cgaaggcatc acggcgaccg
     1141 acgtggtgct cgcgctgacc gaattcctgc gcaaggaaaa ggtggttggc gcctacctgg
     1201 aattccgtgg cgaaggcgca gcggcgctga cgctgggtga ccgcgccacc atctccaaca
     1261 tggcgccgga atacggtgcg accgcggcga tgttcttcat cgaccagaac accatcgact
     1321 acctcaggct caccggccgc agcgacgagc aggtcaagct ggtggaaacc tatgcccggc
     1381 acaccggcct gtgggccgat gcactcaaga ccgccgtgta cgagcgcacg ctgcagttcg
     1441 atctgtcgac cgtggtgcgc aacatggccg gcccctccaa cccgcacaag cgcctgccga
     1501 cctcggcgct ggccgagcgc ggcatcgccg ccaactggga gctgccggcg gacggcaaga
     1561 tgcccgatgg cgcggtcatc atcgccgcca tcaccagctg caccaacacc agcaatccgc
     1621 gcaacgtgat cgccgccggc ctgctggcgc gcaacgccaa cgcgcgcggg ctgacgcgca
     1681 agccctgggt gaagtcctcg ctcgcgcccg gctccaaggt ggtggaaagc tatctcaagg
     1741 aagccaagct gctgccggag ctggaacagc tcggcttcgg catcgttgcg ttcgcctgca
     1801 ccacctgcaa cggcatgtcg ggtgcgctcg atccgaagat ccagcaggag atcgtcgaac
     1861 gcgacctgta cgcgacggcc gtgctgtccg gcaaccgcaa tttcgacggc cgcatccatc
     1921 cgtatgccaa gcaggctttc ctggcgtcgc cgccgctggt ggtggcctat gccattgccg
     1981 gcacggtgcg cttcgacatc gagaaggacg tgctcggtac cgacaagaac ggccagccgg
     2041 tcaggctcaa ggacatctgg ccgaccgatg ccgagatcga tgccgtggtc gccagcagcg
     2101 tcaacccgac gcagttccgc gcggtgtacg aaccgatgtt ccgcaagcag gccgacgacg
     2161 gcgcgaagat caagccgctg tacgactggc gcgcgcagag cacctacatc cgccgcccgc
     2221 cgtactggga gggcgcgctg gccggcgagc gcaccctgcg cggcctgcgc ccgctggcgg
     2281 tgctgcccga caacatcacc accgatcacc tgtcgccgtc caacgccatc ctgatggaca
     2341 gcgccgcggg cgagtacctg cacaagatgg gcctgccgga agaggacttc aattcgtacg
     2401 ccacgcatcg cggcgatcac ctcaccgcgc agcgcgcgac cttcgccaac ccgcagctgg
     2461 tcaacgagat ggcggtggtc gacggcaagg tgaagaaggg atcgctcgcg cgcatcgagc
     2521 cggaaggcaa ggtcacgcgc atgtgggaag ccatcgagac ctacatgcag cgcaagcagc
     2581 cgctgatcat catcgccggt gccgactacg gccagggttc gtcgcgtgac tgggccgcga
     2641 agggcgtgcg cctcgcgggt gtggaagcca tcgtcgccga aggcttcgag cgcatccacc
     2701 gcaccaatct catcggtatg ggctgcctgc cgctggaatt caaacccggc acgacgcggc
     2761 tcacgctgaa gatcgacggc accgagacgt tcgacgtgat tggcgcgcgc aagccgcgcg
     2821 cgacgctgac gctggtgatc caccgcaaga acggcgagag ggtcgaagtg ccggtgacct
     2881 gtcgcctgga ttccgatgaa gaggtttcca tctacgaagc cggcggcgtc ttgcagcgtt
     2941 ttgcgcagga tttccttgaa tcgaacaagg cggcctgaac gaattatctg aggagatgga
     3001 tcaatggcaa tgtcttggcg ggacgcaata gaaaaggttc tgagcgagac ttcgacaccg
     3061 ctgcactatt cggagatcgc agagcaaatc ctaacgcgcg ggtactacaa gaccgcgggt
     3121 gctactccgg ccgcaacggt aaatgcgcag atcacttctt caataaagca tgagggagac
     3181 acatctccct ttgtaaaggt cgcgcgcggg acgtatgcgt taagagaatc atcccgcgtc
     3241 cagccgcgcg ctgccgttga gcccgcacgc caaagcatta acgccagtct tgaagcagag
     3301 attcagtctg actccgagga atccgagtcg gtaatacgca gcttcggtat gtactggcaa
     3361 cgtgaccttg tggtttggaa gggagagcct cgcatgtttg gcaaacagca ggccgcatca
     3421 acgcccgtag attttggcaa gcaaaaaggt ctttacatcc tttacgatca ccacaccgtt
     3481 gtatatgtcg ggcgcgcaat cgatcgccct ctcgggcgtc gactctatga acacacgatt
     3541 gatcgcctcg ccagtcgctg gaacagattt tcttggttcg ggttccatga agttaccgac
     3601 gatggtcggc ttcaagatgt gcccgttaaa acgtccttgg ctgcattggt agcgacactt
     3661 gaagcgttgc tgattgaatc tttggagccg cctcaaaata gaaagcgcgg agacgatttc
     3721 tcggtaaacg agtacatcca ggacggtgat ccagaattga aggaaagaga gcttcagaac
     3781 acgctacgag ccattgagca gaagcttcgc ggaagaaact agattgccga tgaacgaaaa
     3841 atgacaggtg ctccgcaaat caagattccc gccacctaca tgcgcggcgg caccagcaag
     3901 ggtgtgttct tccgcctgca ggatttgccg gccccggtgc agcagcccgg cccggcgcgc
     3961 gacaagttcc tgatgcgcgt gatcggcagc cccgatccat acgcggcgca tatcgacggc
     4021 atgggcggcg cgacttcgag caccagcaag acggtgatca tcggcaagag cgcgcagccc
     4081 ggccacgacg tcgattacct ctacggccag gtgtccatcg acaaggcttt cgtcgactgg
     4141 agcggcaact gcggcaatct ctccacggcg gtggggccgt tcgcggtggc caatggcttc
     4201 atcgagaaat cacggttgcc gaaggacggc gtgttcgcgg tgaaggtgtg gcaggccaat
     4261 atcggcaaga ccatcatctg ccatgtgccg atcaaaaacg gcgaggtgca ggaaaccggc
     4321 gacttcgagc tcgacggcgt gacgttcccg gcggcggaga tcgtgctgga gttcctcgat
     4381 ccttcagacg acggtgacgc gggcggctcg atgtttccga ccgggaatct cgtcgatgac
     4441 ctcgacgtgc ccggcatcgg caggttgaag gcgacgatga tcaccgccgg cattccgacg
     4501 gtgttcgtca atgcggcgga tatcggctac accggcaccg agctgcgcga ggcgatcaac
     4561 accaagcccg aggcgctggc gaaattcgaa gccatccgcg tggccggcgc gctgcgcatg
     4621 gggctgataa agcagcccga ggaagcggcg acgcgccagc acacgcccaa ggtggcgttc
     4681 gtggcgccgc cgaaggatta cgtgtcgtcc agcggcaaga ccatccgggc ttccgagatc
     4741 gatctgctgg tgcgcgcgct gtccatgggc aagctgcacc acgccatgat gggcacggcg
     4801 gcggtggcga tcggtaccgc ggcggcgata cccggcacgc tggtcaatct cgccgccggc
     4861 ggcggccagc gcgaggc
//
LOCUS       DASZZC010000627         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_306209,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000627 DASZZC010000000
VERSION     DASZZC010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306209"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VHE37_13345"
     CDS             <1..380
                     /locus_tag="VHE37_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36567.1"
                     /translation="CEHDGWLYAGTFCWANMLPFLPRYKWPKDVAALVARWGEENLAR
                     RYGGAELFRTHDGVHWEPVTHSGFGNRYNWGVRNFASTPHGLFVGTANIFGPTVAVRR
                     DGHWAYVQNPRGGCEVWLGQAEG"
     gene            377..1234
                     /locus_tag="VHE37_13350"
     CDS             377..1234
                     /locus_tag="VHE37_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36568.1"
                     /translation="MSAPPVLVLGAPFSGAAPLARALGAHPSLYALPQLNLFMADRVS
                     ELIDMYALSQGAHGDGLLRAIAQLHWHGQTDATIGAARKFLAEHEHWSGAELLDDLAA
                     RAAPRRLLLADTESPLRPLDLRRIAALPGAVLIHVTRHPYAQGLMHEAWLRSRLFVPP
                     DFKDHFHTPARIEPQLGWLRANRNLDQHVAAALRVRIEDCVGDAPALDDIGRLLDLPE
                     SAATGAGAFEGYGPTAAPYGLDDELVAEFATSLTEQAQRGTLVSIPPWIDAGFAAEVS
                     ALARRYGYR"
     gene            complement(1351..2112)
                     /locus_tag="VHE37_13355"
     CDS             complement(1351..2112)
                     /locus_tag="VHE37_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36569.1"
                     /translation="MTRKKTSTRVALVTGAGGGIGSVTALEFARHGYRVAVCDIDEAS
                     VQATAAAVKKAGGKARAYVTDVADAHSVERLFAAIEKDFGRLDAAFNNAGVGGARTPV
                     AEVKFDDWERILRINLTGTFLCMQQEIRLMLKQGGGAIVNNGSIFALNGGISAPYTAT
                     KHGIAGLTKSAALSYAQQGVRVNAVCPGLIEAGMGLKVLKRTSTDPKELIAAHPVNRT
                     GTALEVAHAVLWLCSDQAGFVHGHLLAIDGGYGAR"
     gene            complement(2211..3053)
                     /locus_tag="VHE37_13360"
     CDS             complement(2211..3053)
                     /locus_tag="VHE37_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36570.1"
                     /translation="MAFASVNGAKLAYQQLGESGPHLLLVHGLATNRAFWFPHATALS
                     NQFRVTLFDLRGHGYSERTASGYDVSTLARDIVGLMDELKIERAALAGHSYGGAAALE
                     AAGLAPQRISHLALFDVRVTVLQPAMRLHDMPYLSDFEREIAGSAGVDWENETQVGFK
                     FLEAAARRRVSGAETQARDDFTPFGEGRGALRAAKAWVDLLEQTSANTDLHVAGMDAE
                     RIAALPMPLLFMYAQRSRCLPSGHALHALLPRARYEEVPDAGHFFPMSHARLVVDMLR
                     HFLL"
     gene            complement(3053..3328)
                     /locus_tag="VHE37_13365"
     CDS             complement(3053..3328)
                     /locus_tag="VHE37_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVT36571.1"
                     /translation="MTSDQLVPKLVAVLQDFTQDWDNEFEGAMTRETKLLADLGFESI
                     DIIQLVVAIEEDISKRKLPFDQLLMKDGRYVDDLSIGQIADFLAARI"
     gene            complement(3339..>4261)
                     /locus_tag="VHE37_13370"
     CDS             complement(3339..>4261)
                     /locus_tag="VHE37_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT36572.1"
                     /translation="QAWQYLDPALAPKNPQGYDKSMREVCLQYFRNVDGYIKRLVELA
                     GPDAQVFFASDHGFTASTEVLRINSFLEDLGYLKWAVNDGSEHAIRREASDFANLDWS
                     KTTAYCRTPSSNGIHIRVADKPGAPGIAPADYEKFRDKLIADLYTLKDPASGERIVKD
                     VLKREEWFPGAHMKLACDLTLVLRDHGFVSIRNYKPALVKRPNPAGTHHPDGIFMACG
                     KGIAASGMVDSRRIVDVCPTLLHSLGLPVPSDIEGEVPASFFSAEWLAAHPVKKGART
                     QKVGKREGTEMDENEKKQMIEQLQMLGYME"
BASE COUNT          638 a         1413 c         1462 g          748 t
ORIGIN      
        1 tgtgcgagca cgacggctgg ctctacgccg gcacgttctg ctgggccaac atgctgccgt
       61 tcctgccgcg ctacaagtgg cccaaggacg tggccgcgct ggtggcgcgc tggggcgagg
      121 agaatcttgc gcggcgctac ggcggcgcgg agctgtttcg cacgcacgac ggcgtgcact
      181 gggagccggt gacgcactcg ggcttcggca accgctacaa ctggggcgtg cgcaatttcg
      241 cctcgacgcc gcatgggctg ttcgtcggca ccgccaacat cttcggcccg acggtggcgg
      301 tgcgccgcga cggccactgg gcctacgtgc agaatccgcg cggcggctgc gaagtgtggc
      361 tggggcaagc ggaaggatga gcgcgccgcc ggtactggtc ctcggcgcgc cgttctccgg
      421 cgccgcgccg ctggcgcgcg cgctgggcgc gcatccatcg ttgtacgcgc tgccgcagct
      481 caacctgttc atggccgacc gcgtgagcga actcatcgac atgtacgcgc tgtcgcaggg
      541 cgcgcatggc gacggcctgc tgcgcgccat cgcgcagctg cactggcacg ggcagaccga
      601 cgcgaccatc ggcgcggcgc gcaagttcct ggcggagcac gagcactgga gcggcgcgga
      661 gctgctcgac gatctggccg cgcgcgccgc gccgcgccgc ctgctgctgg ccgacaccga
      721 gtcgccgctg cgcccgctgg acctgcgccg catcgccgcg ctgcccggcg cggtgctgat
      781 ccacgtcacc cgccatccgt acgcgcaggg cctgatgcac gaagcgtggc tgcgctcgcg
      841 cctgttcgtg ccgccggatt tcaaggatca cttccacacg cccgcgcgca tcgagccgca
      901 gctgggctgg ctgcgcgcca atcgcaatct tgaccagcac gtggccgccg cgctgcgcgt
      961 gcgcatcgag gattgcgtgg gcgatgcgcc cgcgctggat gacatcggcc ggctgctgga
     1021 tctgccggaa agtgcggcga ccggcgcggg cgccttcgag ggttatggcc cgactgccgc
     1081 gccctacgga ctcgacgacg agctcgttgc ggaattcgca acgtcgctga ctgaacaggc
     1141 gcagcgcgga acgctcgtct cgataccgcc atggattgat gccgggtttg ccgcggaagt
     1201 gagcgcgctc gcgcgccgct acggttatcg ctgagtgaaa aacgcctgca ccgcacccgg
     1261 gatcacgatg ttattaacta ttgtatgaat ataattacgt acaacaatta ataacaggcg
     1321 caagggcagt gcggtcagcc ttttttttgc tcagcgcgcg ccgtagccgc cgtcgatcgc
     1381 cagcaggtgg ccgtgcacga agccggcctg gtccgagcac agccacagca ccgcgtgcgc
     1441 cacttccagc gcggtgccgg tgcggttcac cgggtgcgcg gcaatcaatt ccttcggatc
     1501 agtgctggtg cgcttgagca ccttgagccc catgcctgct tcgatcaggc ccggacacac
     1561 cgcgttgacg cgcacgcctt gctgcgcata cgacagcgcg gccgatttgg tcaggccggc
     1621 gataccgtgc ttggtcgcgg tgtacggcgc gctgatgccg ccgttgagcg cgaagatgga
     1681 gccgttgttg acgatggcgc cgccgccctg cttgagcatc aggcggatct cctgctgcat
     1741 gcacaggaag gtgccggtga ggttgatgcg caggatgcgt tcccagtcgt cgaatttcac
     1801 ctccgccacc ggcgtgcgcg cgccgccgac gccggcgttg ttgaaggcgg cgtcgagccg
     1861 gccgaaatcc ttctcgatgg cggcgaaaag tcgttccacc gagtgcgcgt cagcgacatc
     1921 ggtgacgtag gcgcgcgcct tgccgcccgc cttcttcacc gcggcggcgg tggcctgcac
     1981 gctggcttcg tcgatatcgc aaacggcgac ccggtagccg tgccgcgcaa attccagcgc
     2041 ggtgacgctg ccgatgccgc cgcccgcacc ggtcaccagc gcgacgcgcg tggacgtttt
     2101 ctttctggtc atggattccc ctcgcaggcg cattctacgt gcccgcagtg ctgttgtagg
     2161 agcgcgcgtt gcgcgcgatg cgcggactcg atcgcgcgca aggcgcgctc ctacaggagg
     2221 aaatgacgca gcatgtcgac cactagccgt gcatggctca tcgggaagaa gtggccggca
     2281 tcgggcactt cctcgtagcg cgcgcgcggc agcagcgcgt gcagcgcgtg gcccgagggc
     2341 aggcagcgcg agcgctgcgc gtacatgaac agcagcggca tcggcagcgc ggcgatccgt
     2401 tccgcgtcca tgccggccac gtgcaggtcg gtgttggcgc tggtctgctc cagcaaatcc
     2461 acccaggcct tggccgcacg cagcgcgccg cggccttcgc cgaacggggt gaagtcgtcg
     2521 cgcgcctggg tctccgcgcc ggacacgcgc cggcgcgcgg cggcttccag gaacttgaag
     2581 ccgacctggg tttcgttctc ccagtcgacg ccggcgctgc cggcgatctc gcgctcgaag
     2641 tccgacaggt atggcatgtc gtgcagccgc atggcgggct gcagcacggt gacgcgcacg
     2701 tcgaacagtg cgagatggct gatgcgctgt ggcgccagac cggcggcttc cagcgccgcc
     2761 gcgccaccgt agctgtggcc ggccagcgcc gcgcgttcga tcttcaattc atccatcagc
     2821 ccgacgatgt cgcgagcgag cgtggacacg tcgtagccgc tggccgtgcg ctcgctgtag
     2881 ccgtggccgc gcaggtcgaa cagcgtgacc cggaactggt tcgacagcgc cgtggcgtgc
     2941 ggaaaccaga acgcgcggtt ggtggccagc ccgtgcacca gcagcaggtg cggaccgctc
     3001 tcgccgagct gctggtacgc gagcttcgcc ccgttgacgc tggcgaacgc cactagattc
     3061 tggccgcgag gaagtccgcg atctggccga tggagagatc gtcgacatag cggccatcct
     3121 tcatcagcag ctggtcgaac ggcagcttgc gcttgctgat gtcttcctcg atggcgacca
     3181 ccagctggat gatgtcgatc gattcgaagc ccaggtcggc cagcagtttc gtctcgcgcg
     3241 tcatcgcgcc ttcgaattcg ttgtcccagt cctgggtgaa gtcctgcagg accgcgacga
     3301 gcttgggaac gagttgatcg gaagtcatag ggtgcgaatc attccatata accgagcatc
     3361 tgcagctgct cgatcatctg cttcttctcg ttctcgtcca tctcggtgcc ttcgcgcttg
     3421 cccacttttt gcgtgcgcgc gcccttcttc accgggtgcg cggccagcca ctctgcgctg
     3481 aagaacgacg ccggcacttc gccctcgatg tccgacggca ccggcagtcc cagtgaatgc
     3541 agcagcgtgg ggcagacgtc gacgatcctg cgcgagtcga ccatgccgct ggcggcgatg
     3601 cccttgccgc aggccatgaa gatgccgtcg ggatggtgcg tgccggcggg attgggccgc
     3661 ttcaccagcg ccggcttgta gttgcgaatg gaaacgaagc cgtggtcgcg cagcaccagc
     3721 gtgaggtcgc aggccagctt catgtgcgcg ccggggaacc actcctcgcg cttgagcacg
     3781 tccttgacga tgcgctcgcc gctggccggg tctttcagcg tgtacaggtc ggcgatgagc
     3841 ttgtcgcgga atttctcgta gtcggccggc gcgatgccgg gggcgccggg cttgtccgcc
     3901 acacggatgt ggatgccgtt cgacgacggc gtgcggcagt acgcggtggt cttgctccag
     3961 tcgaggttgg cgaagtcgga ggcctcgcgg cggatggcgt gctcgctgcc gtcgttcacc
     4021 gcccacttca ggtagcccag gtcctcgagg aagctgttga tgcgcagcac ctcggtgctg
     4081 gcggtgaaac cgtggtcgga ggcgaagaac acctgcgcgt cggggccggc cagttcgacc
     4141 aggcgtttga tgtagccgtc gacgttgcgg aaatactgca ggcacacctc gcgcatgctc
     4201 ttgtcgtagc cctgcggatt cttcggcgcc agcgccgggt ccaggtactg ccaggcctgg
     4261 t
//
LOCUS       DASZZC010000628         9856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1146363,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000628 DASZZC010000000
VERSION     DASZZC010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146363"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VHE37_13375"
     CDS             complement(<1..956)
                     /locus_tag="VHE37_13375"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT36573.1"
                     /translation="MKRARAGMLLLCAALAPAARGAQAAAAEDAHALGELSIEQLMNI
                     TVTSVAKKAENLIDAAAAVTALSADDLRRSGAGSVAEALRMVPGMDVAAVDASQWAIS
                     ARGFNELYANNLLVLVDGRAVYTPVFAGTYWDLQQSMLQDLDRIETIRGPGGTLWGAN
                     AVNGVINVVSRSAQDTQGTLQYGSGGTLKQDAGARYGGSIGDDTWYRVFGSYRHSGDF
                     PLADGSPAGDRWSGTQGGFRLDRDAGGQHFTWQGDATRSDLDDGNSDAYNLNTLVRWK
                     RGSAGDGSVELQAYYDRTHRNDTARARTTLDTADVAFQQIVEL"
     gene            complement(953..1369)
                     /locus_tag="VHE37_13380"
     CDS             complement(953..1369)
                     /locus_tag="VHE37_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HVT36574.1"
                     /translation="MSPWTIRHRIILSFAIVVGVILRTSALIQRRLESIRHDAGNILA
                     QSLPGLYQSAQMSFEWELNQELTGEYAIQVQKNGRNDVGAQLQANWKAFAGSLRLYQD
                     TVDTSDEQRQFEDLRSQLAQYQRLRATALFGSGAGR"
     gene            1373..1606
                     /locus_tag="VHE37_13385"
     CDS             1373..1606
                     /locus_tag="VHE37_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36575.1"
                     /translation="MHSLQEWTLERGQQQRRSAALPGKSAEWRASRGRAPPEPRIKPM
                     RLQLFIATLRVVEPPPIKPEATVGRHLSVWPGR"
     gene            1663..1923
                     /locus_tag="VHE37_13390"
     CDS             1663..1923
                     /locus_tag="VHE37_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36576.1"
                     /translation="MNGSNDGKGQQSAAADPAAGIDVLMVDDQPMLGETVRRQPPPDA
                     GRINVSRQVEERVTARWHCEPRGTLPVKTREPLEMFFVNGPR"
     gene            complement(2019..3263)
                     /locus_tag="VHE37_13395"
     CDS             complement(2019..3263)
                     /locus_tag="VHE37_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HVT36577.1"
                     /translation="MKLHWQIGIALALAITAGTLLGPVPWLVDGGGFLGTLFLDALKM
                     IVVPLIVSSLIQALLSLSDPSALSRMGLQAIAYYTVTCALAVVTGLAMINLIKPGIID
                     GAPAAKLLGLTSSSREVLEKIGNRGVADIVNVFQRLVPSNLIDAAARGDILGLIPFSL
                     LYGYFASRLPEPLAATQRAFWQGLQHIMLGVTGVVMRFAPVGVFGLVAKTVALTGWDA
                     IHPLALFFCAVFGGLLVHMFVTMPLTLRLVAGIHPWRHLRAMTPALLTAFSSSSSAAT
                     LPVTLDCLQNRAGVSERVTSFVLPLGATINLDGSALYECAVAMFFAQAYGLELSFATQ
                     FLIVWMALVTSMGISGIPSASLVAIAVILGAIGLPIEGLGVILAIDRILDMCRTAVNV
                     WGDSCGAVVVARLQGEKQVLQA"
     gene            complement(3340..5259)
                     /locus_tag="VHE37_13400"
     CDS             complement(3340..5259)
                     /locus_tag="VHE37_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVT36578.1"
                     /translation="MLQVIRDKLTGWVTWFAIGVIGVPLAFFGIDALRNGGGDPVIVK
                     VGSQEIHESQFKAAYEQRYQQMQQLMGERFRADQFDQERFRQAVLNDMEQESMLRQYT
                     QDAGYRATDGQVFDRIKEIPVFQKDGKFDTDSYTQALNRYGQTPTRFENQLRESIAQD
                     QMREAILDSAFVTPAESAQAWKLAGEQRYLSYATFDPARYEAQVTVSDEQAKARYEEQ
                     KSRYMAPERVKLAYVQLSLDAMPKAEPPKADALKVIYDAQKAGKFSTPEERKARHILL
                     NFGADKSVSKKKIEALAEQIKGGADFSELAKKNSDDPGSKDAGGDLGWVRTGQMVKKF
                     EDTLFSMKKGEVSDPVETEFGWHLIKLDDIHPAAVKPFDDPEVQKQLVDLYQTTEAAK
                     HFQESQEKLDTLAFENPTSLEVPARELGLKIETTDWITRAGGEGITANDAVKQAAFSN
                     EVIKDGDNSKPISLGNNQVVVIRKAEYEAPRQKTYEEVADVIKAELKTEQARAKAQAD
                     ADEVLKAARGGTALADALKGKGAELQSPGLIKRDNTTVDRAIVAALFKLPRPKDSKPQ
                     FGDAHLASGAVAVIGLSAVQEPEAQPATDADNARKRERDVLAGQEFAGYRKQIESVVK
                     IKVVKAPEAEAPAKP"
     gene            5560..6171
                     /locus_tag="VHE37_13405"
     CDS             5560..6171
                     /locus_tag="VHE37_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36579.1"
                     /translation="MRVFAVALLCAGSAWASEPPTDEAELKLDGAIQGLKEEVINFNR
                     DATAVENTVQYPTRTRTDIYLGMQVPALLIKSVKVTIDDLPAQSYTYSDVEARALLNS
                     EGLARIARLSLEPGPHRLAAEYTAQFADAKADAPLVGGKYEAIFDKSGREADLEIRIA
                     RSSRLSDPEVRLREFKPAAPGEKVVPEKPAKRRPIRFRLPFLP"
     gene            6168..8075
                     /locus_tag="VHE37_13410"
     CDS             6168..8075
                     /locus_tag="VHE37_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36580.1"
                     /translation="MKALRYLPLLLAAGAAHAAEVYVPGAKNDPRLRSVAYEAAAQKN
                     LTALTELLELRGERDLGDLPPDYQWQLAQAYVNFGMGTRAQTLYRNLAETAPDNDQLA
                     KARLQLAEFQYERGYWEEARATLYSMREKLPDSAVIRWQDLLSRVLLAEGRYSDAIEV
                     LTGLKNADRQSEYTRYNYGVALINNGDLKNGREVIDRVGRLRPEDVETMSLRDRANLA
                     LGWHYLRAELASNARDAFYRIRSAGPYSNRALLGLGWAELAPGGKTHTRDIPDDLTPF
                     STFATLGGLLKPGFLNRDSSAAGPREFRLDNISPVEAAALRRALAAWVELIQRDPLDP
                     AVQEAYLAIPYSLDRLGAHTEALQYYEKAVEHLEQNRQRLDVAMANLKKYTMVETVVR
                     RDANTESGWQWKLKDLADTPETYYLQNLIAEHRYQEALKNYRDIRLMARVLDTWKSRL
                     TALELAHAAQGGEDVSPEVLFVRAKEGWVEPWKHINIQLRLDTELSPPGITEAPFKSP
                     ADVPVELNLAPVPARFNGPSERIEELRARMANLRELIATAGGQEAQILQDLGTKELMG
                     QKKQVEKYLVEARFALARLYDRQLNPEKAKAEPQPEKKEGLFNRFLHLFGGSDDTART
                     HESEQKKGGGK"
     gene            8072..>9856
                     /locus_tag="VHE37_13415"
     CDS             8072..>9856
                     /locus_tag="VHE37_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT36581.1"
                     /translation="MKQRAFLLGLTLALAACTPRIVKDAAPPIGDVVDKPKPDDHVLP
                     IIPSEQIAVDPQKAADNYRKLLALTPDDKTQAESMRRLADLQVQMEDAKGNDAPESSK
                     SLKEAIQLYNDLLYKQPNDPNNDRVFYQLARAYQNSGDMDAAIDTLQRLTTRYPNSDV
                     AGDAHFRRAELLFARNRWAEAEAEYKTVMDLADATPFFQSAQYKYGWTRYKQANYEGA
                     IETFGQILDRELPQGELYDPKAALDAVARNKSDLARDSLRVTSLSFAALGGGKAVNDY
                     YAKHGDPRYFPLVYSALGDLELEKLRYTEGAETFAAFIERYPNDSHAPAFQSRVIKAY
                     DAGGFHDLVAREKERYVTTYDPAAPYWHGTATTAAVMSELRVHLEDLARHYYARGQGA
                     PKPAEGETAQPSPDFLVAAKWYRRTIELYPKDPQVAELNFMLGESLYNGGRTLDAAHE
                     YLKTAYGYPLNRRSDEAAYAAVLALERYSKEVKPEERPAALKETIAASLKLADTFPRH
                     PQVARVLTRSAEDLYELKDYDQAIAVAARVINFPREVDINLRRSVWSVTADSYFAQKK
                     YPEAEKAYTQELALTARNTVAYGQVTEQL"
BASE COUNT         1722 a         3360 c         3161 g         1613 t
ORIGIN      
        1 agttcgacga tctgctggaa ggcgacatcc gcggtgtcca gcgtggtgcg cgcccgcgcg
       61 gtgtcgttgc gatgcgtgcg gtcgtagtac gcctgcaact cgacgctgcc gtcgccggca
      121 ctgccgcgct tccagcgcac cagcgtattg aggttgtagg catcggagtt gccgtcgtcg
      181 aggtccgagc gcgttgcatc gccctgccag gtgaaatgct gcccgccagc atcccggtca
      241 aggcggaagc caccctgtgt cccgctccag cgatcgcccg ccgggctgcc gtcggccagg
      301 ggaaaatcgc cgctgtgccg gtaactgccg aatacgcgat accaggtgtc gtcgccgatg
      361 ctgccgccat aacgcgcgcc tgcatcctgc ttcagcgtgc cgcccgaccc atactgcagc
      421 gtgccctgcg tatcctgcgc gctgcggctg accacgttga tcacgccgtt gacggcgttg
      481 gcgccccaca gcgtgccgcc tggtccgcgg atcgtctcga tgcggtcgag gtcctgcagc
      541 atggattgct gcaggtccca gtaggtgccg gcgaacaccg gcgtgtacac cgcgcgcccg
      601 tcgaccagca ccagcaggtt gttggcatac agctcgttga agccgcgtgc ggagatcgcc
      661 cactggctgg catcgacggc ggcgacgtcc atgcccggca ccatgcgcaa cgcttcggcc
      721 acgctgcccg ccccggatcg gcgcagatcg tcggcggaca gcgcggtaac ggcagcggcc
      781 gcatcgatca gattttccgc cttcttcgcc accgacgtga cggtgatgtt catcagctgc
      841 tcgatgctca gttcgccgag ggcgtgggcg tcctcggcgg cagccgcctg cgcgcccctg
      901 gcagccggcg ccagggccgc gcagagcagc agcatgcccg cgcgggcgcg cttcaccgcc
      961 cggctcccga tccgaagagc gcggtggccc tgaggcgctg gtactgcgcc agctggctgc
     1021 gcagatcctc gaactgccgc tgctcgtcgg acgtatcgac cgtgtcctgg taaagcctga
     1081 gcgatccggc gaatgccttc cagttggcct gcagttgcgc gccgacgtca ttccgcccgt
     1141 tcttctgcac ctggatcgcg tattccccgg tcagctcctg gttgagctcc cattcaaacg
     1201 acatctgggc gctctggtag agcccaggca gcgactgcgc caggatgttg ccggcgtcgt
     1261 gcctgatcga ctccaggcgg cgctggatga gcgcgctcgt ccgcaggatc acgccgacca
     1321 cgatggcaaa gctgaggatg atgcggtggc ggatggtcca tgggctcatg tcatgcactc
     1381 cctgcaggaa tggacgctgg aacgcgggca gcagcagcgg cgcagtgctg cgctgcctgg
     1441 aaaatccgcg gaatggcggg cctcacgcgg ccgggctcct cctgagccgc gcatcaagcc
     1501 gatgcgcctg cagcttttca tcgccaccct ccgtgtggtc gaaccgccgc ctataaaacc
     1561 tgaagcgacg gtgggtcgcc acctgtccgt ttggcccggt cggtgacggc cctatactgc
     1621 tcctggcgct aagattctcc ttgcaagggc ctgcgtcaag tcatgaacgg aagcaacgac
     1681 ggcaaaggac agcagtcggc ggcggcggac ccggctgcgg gcatcgatgt gctgatggtg
     1741 gacgatcagc cgatgctggg cgaaaccgtg cggcgacagc cgccgccgga cgccggccgc
     1801 atcaacgtgt cgcgccaggt tgaggagcgc gtcactgcgc gctggcattg cgagccgcgc
     1861 ggcacgctgc cggtgaagac ccgcgagccg ctggagatgt ttttcgtcaa cggaccacgc
     1921 tgaccgcacc ttgctttttt atgttataaa ataataactt attatttatt tgtattatat
     1981 ttaataacat caccgatgca cgtgcggtga catggttttc aggcttgcag cacctgcttt
     2041 tcgccctgca gccttgccac cacgacggcg ccgcacgagt ctccccacac gttcaccgcg
     2101 gtgcggcaca tgtcgaggat gcggtcgatg gccaggatca ccccgagtcc ttcgatcggc
     2161 agcccgatgg cgcccaggat caccgcgatc gccaccaggc tggcggacgg aatcccggag
     2221 atgcccatcg aggtcacaag cgccatccac acgatgagga actgggtggc gaacgacagt
     2281 tccagcccgt aggcctgggc gaagaacatc gccaccgcgc actcgtacag cgcgctgccg
     2341 tcgaggttga tggtggcgcc cagcggcagc acgaagctgg tgacgcgctc ggatacgccg
     2401 gcgcggttct gcaggcagtc cagcgtcacc ggcagcgtgg ccgccgatga cgatgaggag
     2461 aacgcggtga gcagcgcggg cgtcatcgca cgcagatgac gccacggatg gatgccggcc
     2521 accagccgca gcgtgagcgg catggtgacg aacatgtgca cgagcagccc gccgaacacc
     2581 gcacagaaga acagcgccag cgggtggatc gcatcccagc cggtgagcgc cacggtcttg
     2641 gccaccaggc cgaacacgcc gaccggcgcg aacctcatca ccacgccggt cacgccgagc
     2701 atgatgtgct gcagtccctg ccagaacgcg cgctgcgtcg ccgccagcgg ctcgggcagg
     2761 cgcgaggcga agtagccgta gagcagcgag aacgggatca atcctaggat gtcgccgcgg
     2821 gcggcggcgt cgatcaggtt cgacggcacc aggcgctgga acacgttgac gatgtcggcc
     2881 acgccgcggt tgccgatctt ctccagcacc tcgcggctcg acgaggtgag gccgagcagt
     2941 ttcgccgccg gcgcgccgtc gatgatgccc ggcttgatca ggttgatcat ggccaggccg
     3001 gtgaccaccg ccagcgcgca ggtcactgtg tagtaggcga tggcctgcag ccccatgcgc
     3061 gacaacgccg aggggtcgga caggctgagc agcgcctgga tcagcgagga gacgatcagc
     3121 ggcaccacga tcatcttcag cgcgtcgagg aaaagagtgc caagaaatcc gccgccgtcg
     3181 accagccacg gcaccggccc gagcaacgtg ccggcggtga tagccagcgc cagcgcaatg
     3241 ccgatctgcc agtgcagttt catgggcgct gcatctcccc gaaatgaaaa agccgttcgc
     3301 gccagccaag cacgaacggc tgcgtccgtc gtgggcggat cagggcttgg ccggcgcctc
     3361 ggcttccggt gccttcacca ccttgatctt caccacgctc tcgatctgct tgcgatagcc
     3421 ggcaaattcc tggccggcca gcacgtcgcg ctcgcgcttg cgtgcattgt cggcgtccgt
     3481 tgccggctgc gcttccggct cctgcaccgc cgacaggccg atcaccgcca cggcgccgct
     3541 ggccaggtgg gcatcaccga actgcggctt gctgtccttg ggccgcggca gtttgaacag
     3601 ggcggcaacg atcgcacggt ctaccgtggt gttgtcgcgc ttgatcagtc cgggcgactg
     3661 cagttcggcg cccttgccct tgagcgcatc ggccagcgcg gtgccgccgc gcgcggcctt
     3721 gagtacttcg tcggcatcag cctgggcctt ggcgcgcgcc tgctcggtct tcaactcggc
     3781 cttgatgacg tcggcaacct cttcataggt tttctgccgc ggcgcttcgt actcggcctt
     3841 gcggatcacc accacctggt tgttgcccag cgagatcggc ttgctgttgt cgccgtcctt
     3901 gatcacttca ttggagaacg cagcctgctt caccgcgtcg ttggcggtga tgccttcgcc
     3961 gcccgcccgc gtgatccagt cggtggtctc gatcttcagg cccagttcgc gcgccggtac
     4021 ctccagcgaa gtcgggttct cgaaggccag cgtatcgagc ttctcctgcg attcctggaa
     4081 atgcttggcc gcctcggtgg tctggtacag atccaccagc tgcttctgca cctcgggatc
     4141 gtcgaacggc ttgaccgccg ccgggtggat gtcgtcgagc ttgatcaggt gccagccgaa
     4201 ctcggtttcc accgggtcgc tcacctcgcc cttcttcatc gagaacagcg tgtcctcgaa
     4261 cttcttcacc atctgcccgg tacgcaccca gcccaggtcg cccccggcgt ccttggaacc
     4321 cgggtcatcc gaatttttct tggccagttc ggagaaatcc gcgccgccct tgatctgctc
     4381 ggccagcgcc tcgatcttct tcttcgacac gctcttgtcg gcgccgaagt tgagcaggat
     4441 gtgccgcgcc ttgcgctctt ccggcgtgga gaacttgccg gccttctgcg cgtcgtagat
     4501 caccttgagc gcgtcggcct tgggcggctc ggccttgggc atggcgtcga gcgacagctg
     4561 cacgtaggcc agcttgacgc gctccggcgc catgtagcgg ctcttctgct cttcgtagcg
     4621 cgctttcgcc tgctcgtcgc tgacggtcac ctgtgcctcg tagcgggccg gatcgaacgt
     4681 ggcgtacgac aggtaacgct gctcgcccgc gagcttccac gcctgcgcgc tctccgccgg
     4741 cgtgacgaag gcggaatcga ggatcgcctc gcgcatctgg tcctgggcaa tcgactcgcg
     4801 cagctggttc tcgaagcgcg tcggcgtctg gccgtagcgg ttgagcgcct gcgtatagct
     4861 gtcagtgtcg aacttgccgt ccttctggaa taccgggatt tccttgatgc ggtcgaacac
     4921 ctgcccgtcg gtggcgcggt agccggcatc ctgcgtgtac tggcgcagca tcgactcctg
     4981 ttccatgtcg ttgagcaccg cctggcggaa gcgctcctgg tcgaactggt cggcgcggaa
     5041 acgctcgccc atgagctgct gcatctgctg gtagcgctgc tcgtaggccg ccttgaactg
     5101 agactcgtgg atttcctggc tgccgacctt gacgatcacc gggtcgccgc cgccgttgcg
     5161 cagcgcgtcg atgccgaaga aggccagcgg tacgccgatg acgccgatgg caaaccaggt
     5221 gacccagccg gtcagcttgt cgcgtatgac ttgcagcatg tcgtatccga agttttttgg
     5281 gtgcgcggat gggtccgcgg ctattcgtgc aaggcccggc acgccgggcg ctttttgagg
     5341 gcgcgaagta tagcggattt gcctgcgcaa accggccgcg cggcgcgccg gctaaacaaa
     5401 acgcatcacg cgcctgtgca tggcgcgccg ttcatcgcgt ccacatggtc ggtgagtcag
     5461 ctgacacagt caggcgaggc gcatttgtta gactggtatg cgaaggatcg aatcaactcc
     5521 cgttcgtcac tcatgacact gctacagggc cgtcgcggta tgagagtttt tgcggtcgcg
     5581 ctgctttgcg cgggcagcgc gtgggcgtcg gaaccgccta ccgacgaggc agaactcaag
     5641 ctcgacggcg ccatccaggg gctcaaggaa gaagtcatca acttcaaccg agatgccacg
     5701 gcggtcgaga acaccgttca gtatccgacc cgcacgcgta ccgacatcta cctgggcatg
     5761 caggtgccgg cgctgctgat caagtccgtc aaggtcacca tcgacgacct gccggcgcag
     5821 agctacacct acagcgacgt cgaggcgcgc gcgctgctca acagcgaagg cctggcgcgc
     5881 atcgcgcgcc tgtcgctgga acccggcccg caccggctgg ccgccgaata caccgcgcag
     5941 ttcgccgacg ccaaggccga tgcgccgctg gtcggcggca agtacgaagc catcttcgac
     6001 aagtccggcc gcgaggctga cctcgagatc cgcatcgcgc gcagctcgcg gctgtccgac
     6061 cccgaggtgc gcctgcgcga attcaagccg gccgccccgg gcgagaaagt ggtgcccgaa
     6121 aagccggcca agcgccgccc catccgcttc cgcctgccgt tcttgccatg aaggccctgc
     6181 gctatctgcc cctgctgctc gccgccggcg ccgcgcatgc cgcggaagtc tatgtgcccg
     6241 gcgccaagaa cgatccgcgc ctgcgttcgg tggcttacga ggcagcggca cagaagaatc
     6301 tcaccgccct gaccgagctg ctggaactgc gcggcgaacg tgacctgggc gacctgcctc
     6361 ctgattacca gtggcagctg gctcaggcct acgtcaactt cggcatgggc acgcgcgcgc
     6421 agacgttgta ccgcaacctc gcggaaaccg ctcctgacaa cgaccagctg gccaaggcgc
     6481 gcctgcagct ggcggaattc cagtacgaac gcggctactg ggaggaagcg cgggccaccc
     6541 tgtactcgat gcgcgagaag ctgccggaca gcgcggtcat ccgctggcag gatctgctgt
     6601 cgcgcgtgct gctggcggaa ggccgctatt ccgacgccat cgaggtgctc accggcctga
     6661 agaacgccga ccgccagagc gaatacacgc gctacaacta cggcgtggcg ctgatcaaca
     6721 acggcgatct caagaatggc cgcgaggtga tcgaccgcgt cggccgcctg cgcccggaag
     6781 acgtcgagac catgtcgctg cgcgaccgcg ccaacctggc gctgggctgg cattacctgc
     6841 gcgcggaact ggccagcaac gcgcgcgacg cgttctaccg catccgcagc gccgggccgt
     6901 acagcaaccg cgcgctgctg ggcctgggct gggccgagct cgcccccggc ggcaagacgc
     6961 atacgcgcga cattcccgac gacctgacgc cgttctccac tttcgccact ctgggcggtc
     7021 tgctcaagcc cggcttcctc aaccgcgact ccagtgcagc cggcccgcgc gaattccgcc
     7081 tcgacaacat ctcgccggtt gaggccgccg cgctgcgccg cgcgctggcg gcctgggtgg
     7141 agctgatcca gcgcgatccg ctcgatccgg ccgtgcagga agcctacctg gccatccctt
     7201 attcgctcga ccgcctgggc gcacacaccg aggcgctgca gtactacgag aaggcagtgg
     7261 agcatctcga gcagaaccgc cagcgcctcg acgtggcgat ggccaacctg aagaagtaca
     7321 ccatggttga aaccgtggtg cggcgcgatg ccaacaccga gtccggctgg cagtggaagc
     7381 tcaaggacct ggccgatacg ccggaaacct actacctgca gaacctgatc gccgaacacc
     7441 gctaccagga ggcgctgaag aactaccgcg acatccgctt gatggcgcgc gtgctcgaca
     7501 cctggaagtc gcgcctgacc gcgctggaac tggcgcacgc ggcgcagggc ggcgaagacg
     7561 tctcccccga ggtgctgttc gtgcgcgcca aggaaggctg ggtggagccg tggaagcaca
     7621 tcaacatcca gctgcggctc gacaccgaac tctctccgcc cggcatcacc gaggcgccgt
     7681 tcaagtcgcc ggccgacgtg ccggtggagt tgaacctcgc gccggtgccg gcccggttca
     7741 acggaccgtc cgaacgcatc gaggaactgc gcgcgcgcat ggccaacctg cgcgagctga
     7801 tcgccaccgc cggcgggcag gaggcgcaga tcctccagga tctcggtacc aaggaactga
     7861 tgggccagaa gaagcaggtg gagaaatacc tggtcgaggc gcgcttcgcc ctcgcccgcc
     7921 tgtatgaccg ccagctcaat cctgaaaagg ccaaggccga gccgcagccg gaaaagaagg
     7981 aaggcctgtt caaccgtttc ctgcacctgt tcggcggcag cgatgacacc gcgcgcaccc
     8041 acgaaagcga gcagaagaaa ggaggcggca agtgaaacag cgcgccttcc tgctcggcct
     8101 caccctggcg ctggccgcct gcacgccccg catcgtcaag gacgcggcgc cgccgatcgg
     8161 cgacgtggtc gacaagccca agcccgatga ccacgtgctg ccgatcatcc cgtcggagca
     8221 gatcgcggtc gacccgcaga aagccgcgga caactaccgc aagctgcttg cgctgacgcc
     8281 cgacgacaag acgcaggccg agtcgatgcg ccgcctggcc gatctgcagg tgcagatgga
     8341 agatgccaag ggcaacgacg cgccggagag cagcaagtcg ctgaaggaag cgatccagct
     8401 gtacaacgac ctgctgtaca agcagcccaa cgatccgaac aacgatcgcg tgttctacca
     8461 gctggcgcgc gcctaccaga acagcggcga catggacgcg gccatcgaca cgctgcagcg
     8521 cctgaccacg cgctacccca attccgatgt tgccggcgac gcgcatttcc gccgcgccga
     8581 gctgctgttc gcgcgcaacc gctgggccga ggccgaggcc gaatacaaga cggtgatgga
     8641 cctggccgac gccacgccgt tcttccagag cgctcagtac aagtacggct ggacccgcta
     8701 caagcaggcc aactatgaag gcgccatcga gaccttcggc cagatcctcg accgcgaact
     8761 gccgcagggc gaactgtacg atcccaaggc tgcgctcgac gcggtggcgc gcaacaagag
     8821 cgacctggcg cgcgattcgc tgcgcgtcac ctcgctgtcc ttcgctgcgc tgggcggcgg
     8881 caaggcggtc aacgactact atgcgaaaca cggcgacccg cgctatttcc cgctggtgta
     8941 cagcgcgctg ggcgacctcg agctggagaa gctgcgctac accgagggcg cggagacctt
     9001 tgccgccttc atcgaacgct acccgaacga cagccacgcg ccggcgttcc agtcgcgcgt
     9061 gatcaaggcc tacgacgccg gcggcttcca cgacctggtg gcgcgcgaga aggagcgcta
     9121 cgtcaccacc tacgacccgg ccgcgccgta ctggcacggc acggcaacga cggcggcggt
     9181 gatgtcggaa ctgcgcgtgc acctagaaga cctggcccgg cattactacg cgcgcggcca
     9241 gggcgcgcca aagccggcgg aaggcgaaac ggcgcagccc tcgccggact tcctggtggc
     9301 ggccaagtgg taccgccgca ccatcgagct gtatccgaag gacccgcagg tggccgaact
     9361 caacttcatg ctcggcgagt cgctgtacaa cggcggccgc acgctcgatg ctgcgcacga
     9421 atacctgaag accgcgtacg gttacccgct caatcgccgc tcggacgaag cggcctacgc
     9481 cgcggtgctg gcgctggagc gctacagcaa ggaagtgaag ccggaagagc gcccggccgc
     9541 gctcaaggaa accattgcag cgagcctcaa gctggccgat accttcccca ggcatccgca
     9601 ggtggcgcgc gtgctcacgc gcagcgccga agacctgtac gagctgaagg actacgacca
     9661 ggcgatcgcc gtcgccgcgc gcgtgatcaa cttcccgcgc gaggtcgaca tcaacctgcg
     9721 ccgcagcgtg tggagcgtga ccgccgactc ctacttcgcg cagaagaaat accccgaggc
     9781 tgagaaggcc tacacgcagg agctggcgct caccgcgcgc aacaccgtgg cctacggcca
     9841 ggtcaccgaa cagctg
//
LOCUS       DASZZC010000629         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_245228,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000629 DASZZC010000000
VERSION     DASZZC010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245228"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..>1502
                     /locus_tag="VHE37_13420"
     CDS             179..>1502
                     /locus_tag="VHE37_13420"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HVT36582.1"
                     /translation="MRKVSLMRGTVGVVCALAAANAAAFEFELGGTQIHLDNLVTAGA
                     VMRMQDRDPTLIGKPNVTPGLCQVRTSGAPAAGAQPLPDYAHDPGQSTNAYAPGLIGA
                     ACATSETADITLFNNSPGSFSVNGDNGDLNFNKHQLVQATAKLTTDLSFNIAGFNVFA
                     RGLYFFDQRYHGKVETHPDTTMAPLHTDFSDAGKRDLGTRAQVLDYFVSRVFDVGDHQ
                     LSVKVGDQVLNWGESSFLIANSLNSLSPPNQALLRLPGFDIKELFQPVGMVYASGTIT
                     QGVSLEGFYQYQWKPVVADPVGSFFSTSDTLGDGGSLAMLSFARAPEDYGYPINDPRY
                     PAGRRGYYRAIDTCNLANFNPSGPDLPCFDSAGTLGSTSSRSIFRDHAEEKRREPPGT
                     GQYGMALKVFADSLNGGTEFGFYFANYHSRFPVVSAFAALDTCITNQAT"
BASE COUNT          261 a          517 c          481 g          243 t
ORIGIN      
        1 acttgctgga tggcggtgcg gtgcaggcct gcagtggcgg atttacctgt cgcgggctct
       61 ggtttgcatg agcggcgagc ggctatgctt gccgcacaaa caagaacggc ggcacgcaaa
      121 cggggtctgc agggattgcg cgccgccacc gaagcatggc gggtacgggg agaggagcat
      181 gcgcaaggtc agcttgatgc ggggaaccgt gggcgtcgtc tgtgcgctgg cggcggccaa
      241 tgccgcggcg ttcgagttcg agctcggcgg cacgcagata cacctggaca acctggtcac
      301 ggcgggcgcg gtgatgcgca tgcaggaccg tgaccccacg ctgatcggca agccgaacgt
      361 gacgccgggc ctgtgccagg tgcgcaccag cggagcgccc gcggccggcg cgcagccgct
      421 gccggattac gcgcatgatc cgggccagag caccaacgcc tacgcgccgg ggctgatcgg
      481 cgcggcctgc gccaccagcg agacggcgga catcacgctg ttcaacaaca gcccgggctc
      541 gttctcggtg aacggcgaca acggcgatct caacttcaac aagcaccagc tggtgcaggc
      601 cactgccaag ctcaccacgg acctgtcgtt caacatcgca ggcttcaacg tgttcgcgcg
      661 cgggttgtac ttcttcgacc agcgctacca cggcaaggtg gagacgcatc ccgacaccac
      721 catggcgccg ctgcacacgg atttctccga cgcaggcaag cgcgatctcg gcacgcgcgc
      781 gcaggtgctc gactacttcg tcagccgcgt gttcgacgtc ggcgatcacc agctgtcggt
      841 gaaggtgggc gaccaggtgc tcaactgggg cgagagctcg ttcctcatcg ccaactcgct
      901 caacagcctg tcgcccccca accaggcgct gctgcgcctg ccgggcttcg acatcaagga
      961 actgttccag ccggtgggca tggtctacgc cagcggcacc atcacccagg gcgtttcgct
     1021 ggaaggcttt taccaatacc agtggaagcc ggtggtggct gacccggtcg gcagcttttt
     1081 ctccacctcc gacacgctgg gcgacggcgg ctcgctggcc atgctgtcgt tcgcgcgcgc
     1141 gccggaggat tacggttacc ccatcaacga cccgcgctat cccgccgggc gtcgcggtta
     1201 ctaccgcgcc atcgacacct gcaacctggc caacttcaat ccatcagggc cggacctgcc
     1261 gtgcttcgac agcgccggca cgctcggttc aacttccagc cgctcgatct tccgcgacca
     1321 cgccgaggag aaacggcgcg agccgccggg caccggccag tacggcatgg cgctcaaggt
     1381 gttcgccgac tcgctcaacg gcggcaccga gttcggtttc tacttcgcca actaccactc
     1441 gcgcttcccg gtggtgagcg cgttcgccgc gctcgatacc tgcatcacca accaggccac
     1501 ca
//
LOCUS       DASZZC010000630         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_874241,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000630 DASZZC010000000
VERSION     DASZZC010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874241"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1938)
                     /locus_tag="VHE37_13425"
     CDS             complement(<1..>1938)
                     /locus_tag="VHE37_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36583.1"
                     /translation="LAGLAANVPSMTVEPFPTHNATLRMFIRGVGTADAQVTQDPAVG
                     VYVDGVYIARSVGLAVDLADLERIEVLRGPQGTLYGRNTTGGAINLITKRPTPGPFSM
                     AQQLTYGGRTLWLSKSSCNIPLGDSAAAKLAVLGNVQDGYVDNTGPGGDFGDRRQWAV
                     RFDARWLGPDGLTADYSYDQSDMRYVNGQFQAMLPPNTNKGLAEFFKPYAAEQSVFSS
                     TRLASLATGAPDQDARSRVHGHSLTLQQPFDDGALLKYIGAWRSLTDDEYIDLGGGGG
                     SLGYRLDTGAYDGPSTTLVYGRPTPLVSPQVYQSQWSHELQYSGKLFDDTLEIIAGAF
                     YFEERGGEHGGPTHHILNARIDPQQAQPLFAFAPQLEQLFVNGAAPNLAAYWDYDLGI
                     HNTAEALYTQGTWTPHWLAQRLHFTVGLRQSWDTREAVKSFVQRQFLEGHTALGDVAA
                     VEIPAAVPKRIVPVSDVFNDVHAARRDHDFSPSANLQYDVTQGATTYLSFARAYKSGG
                     FNIRDPQVSAESGPASDGTNYGFGFVEGFRPEIVESLELGIKSEWLGRRLRLNADVFD
                     SRYKDMQTNFLIPGTISDTKSKNVGKARMRGLEIETGVVPAQGLVLGFDYSRLDARVL
                     EVLDLNGNNVASLYPFVSAPP"
BASE COUNT          298 a          653 c          663 g          324 t
ORIGIN      
        1 tgcggcggcg cggagacgaa aggatagaga ctggccacgt tgttgccgtt gaggtcgagc
       61 acctcaagca ccctggcgtc gaggcgcgaa taatcgaaac ccagcaccag gccctgcgcc
      121 gggaccacac cggtctcgat ctccagcccg cgcatgcgcg ccttgccgac attcttgctc
      181 ttggtgtcgg agatggttcc cgggatcagg aagttggtct gcatgtcctt gtagcggctg
      241 tcgaacacgt cggcattcag gcgcaggcgc cggcccagcc attcgctctt gatgcccagc
      301 tccagcgatt cgacgatctc gggccggaag ccctcgacga aaccgaatcc ataattggtg
      361 ccgtccgagg ccgggcccga ctccgccgac acctgcggat cgcggatgtt gaagccgccg
      421 ctcttgtagg cgcgcgcgaa gctcaggtag gtggttgccc cctgcgtcac gtcgtactgc
      481 aggttggccg agggcgaaaa gtcgtggtcg cgcctggccg cgtgcacatc gttgaacacg
      541 tcgctcaccg gcactatgcg cttgggcacc gcggcgggta tctccaccgc ggccacgtcg
      601 cccagcgcgg tgtgcccttc caggaactgg cgctgcacga agctcttcac cgcctcgcgc
      661 gtgtcccacg actggcgcag gcccacggtg aaatgcaggc gctgcgccag ccagtgcggc
      721 gtccacgtgc cctgcgtgta cagcgcttcc gccgtgttgt ggatgcccag gtcgtaatcc
      781 cagtacgccg ccaggttggg cgcggcgccg ttgacgaaca gctgctccag ctgcggcgca
      841 aaggcgaaca gcggctgcgc ctgctgcgga tcgatacgcg cgttcaggat gtggtgtgtc
      901 ggcccgccgt gctcgccgcc gcgctcctcg aagtagaacg cgccggcgat gatctccagc
      961 gtgtcgtcga acagcttgcc gctgtactgc agctcgtgcg accactgcga ctggtacacc
     1021 tgcggactga ccagcggcgt cggccggccg tagaccagcg tggtcgacgg gccgtcgtag
     1081 gcgccggtgt cgagccggta gccgagcgaa ccgccgccgc cgcccaggtc gatgtactcg
     1141 tcgtcggtca ggctgcgcca ggcgccgatg tacttgagca gcgcgccatc gtcgaacggc
     1201 tgctgcaggg tgagcgagtg gccgtgcacg cgcgagcgcg cgtcctggtc cggcgcgccg
     1261 gtggccagcg aggccaggcg cgtgctggaa aacaccgact gctcggcggc gtagggcttg
     1321 aagaattccg ccaggccctt gttggtgttc ggcggcagca tcgcctggaa ctggccgttg
     1381 acgtagcgca tgtcggactg gtcatacgaa tagtccgcgg tcagcccgtc cgggccgagc
     1441 cagcgcgcat cgaagcgcac cgcccactgg cggcggtcgc cgaagtcgcc gccggggccg
     1501 gtgttgtcca cgtaaccatc ctgcacattg cccagcaccg ccagcttggc tgcggcgctg
     1561 tcgcccagcg ggatattgca gctgctcttg gacaaccaca gggtgcgccc gccgtaagtg
     1621 agctgctgcg ccatggagaa cgggcccggt gtcggccgtt tggtgatcag gttgatggca
     1681 ccaccggtcg tgttgcgccc gtacagcgtg ccctgcgggc cgcgcagcac ctcgatgcgt
     1741 tcgagatcgg ccaggtccac cgcgagcccc accgagcgcg cgatgtacac gccgtcgacg
     1801 taaacgccca ccgcagggtc ctgcgtcacc tgcgcatcgg cggtgccgac gccgcggatg
     1861 aacatgcgca gcgtcgcgtt gtgcgtgggg aacggttcca ccgtcatgct gggcacgttg
     1921 gcggcgagcc cggccaga
//
LOCUS       DASZZC010000631         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_245306,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000631 DASZZC010000000
VERSION     DASZZC010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245306"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VHE37_13430"
     CDS             <1..523
                     /locus_tag="VHE37_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36584.1"
                     /translation="IDPSDSSTIYVTLGGYSTARWAPPGQFQDQNPDVGGGHVFVSHD
                     AGENFIDVSGDLPDTIVSYLVRRGQQMIVGTDIGVFISGDLSGASWAPLGDLPNVPVN
                     QLVLKPGDDSELYAATFGRGVQLYKFGTSSSPVPASSSGERSRFGGAMAPWLLVTMVL
                     MRGLRAATRLRRR"
     gene            complement(580..867)
                     /locus_tag="VHE37_13435"
     CDS             complement(580..867)
                     /locus_tag="VHE37_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36585.1"
                     /translation="MNRSTRMLLALALLSGGSLALAGQNYIVAQQDRSGPAPIVRDDH
                     RSTGLSCAEASPATSDLDDYVGAHFESLQGAPVAFPAQALNKLKQARSPAS"
     gene            complement(1042..1236)
                     /locus_tag="VHE37_13440"
     CDS             complement(1042..1236)
                     /locus_tag="VHE37_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36586.1"
                     /translation="MDRLSQVITLDCPSCRKPLEYPLRWFYRQSTCSHCRADLNQVLY
                     PLLSAIQDRFDDPRDHLRLA"
     gene            1409..1618
                     /locus_tag="VHE37_13445"
     CDS             1409..1618
                     /locus_tag="VHE37_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36587.1"
                     /translation="MNTKIEKLESALRTRIAGLKDWLDQNGRGCREKQAHLTEDSDER
                     AYWHYGYWVALVDLLALLTAEDSSG"
     gene            complement(1558..1956)
                     /locus_tag="VHE37_13450"
     CDS             complement(1558..1956)
                     /locus_tag="VHE37_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36588.1"
                     /translation="MPIEGYRGPYRLTPELINERVVHTGPGAYALGNDNGYTFSIRFV
                     GCADDLGRALREHADRQRYRHFEFRYVLSARIGFEQQCRLYHTYTPPDNPVHPQRPAG
                     TDWCCPVCNPAQPEESSAVSRASKSTKATQ"
     gene            complement(2011..2997)
                     /locus_tag="VHE37_13455"
     CDS             complement(2011..2997)
                     /locus_tag="VHE37_13455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVT36589.1"
                     /translation="MDQLLHNIHDWIVASFGPEVDWKQVFLIGMSPIFLITFMIEFAV
                     EKQRGRGAQFYWKEILANLSLGAGYQVMEVLMWLLVTGAIFGWVYSHRLFTIPVNGWT
                     VIPIFVLVEFCYYWFHRSSHRVRWWWSAHVPHHSGEVMNFTTAMRQSLLNAVVGIFIF
                     YLPPVWLGVPPAVVMFMLAVDLAYQYFVHTEAIGRLPAWYEYIFDTPSNHRVHHGRNP
                     QYIDKNYGGVLIIFDRWFGTYEPEVEKVDYGIPRQVRSYNFFVLNFHEFVDMWRDVLS
                     PGPLLQRLRHLWMPPDWERPGHARIHTWSTHVHPRVPQKGAPTGPLVPAESR"
     gene            complement(3021..>3318)
                     /locus_tag="VHE37_13460"
     CDS             complement(3021..>3318)
                     /locus_tag="VHE37_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36590.1"
                     /translation="ARAWLQGNGRADWADEVEAMIVQHHKVTPYRSSALAEAFRKADM
                     ADVSHGLVAYGLPHALLREAFAAFPNAGFHKRLVQLFAQRLRTHPLSPMPMLRW"
BASE COUNT          625 a         1024 c         1083 g          586 t
ORIGIN      
        1 catcgatccc agcgattcga gcaccattta cgtcacgctg ggcggctact ccaccgcgcg
       61 ctgggctccg cccggacagt tccaggacca gaacccggac gtgggcggtg gccacgtttt
      121 cgtgtcgcac gatgccggcg agaacttcat cgacgtcagc ggcgatctgc ccgacaccat
      181 cgtcagctac ctggtgcggc gcggccagca gatgatcgtg ggcaccgaca tcggtgtgtt
      241 catatccggc gatctgtcgg gtgccagctg ggcgccgctt ggcgacctgc ccaacgtgcc
      301 ggtgaaccag ctggtgctca agccgggcga cgacagcgag ctgtacgcgg ccaccttcgg
      361 ccgtggcgtg cagctgtaca agttcggcac ctcgtcatca ccggttccgg cgtcgtcgtc
      421 gggcgagcgt tcgcgcttcg gcggcgcgat ggcgccctgg ctgctggtga cgatggtgct
      481 catgcgcggc ctgcgcgcag caacccggct gcgccggcgc tgaacataaa aaagcgcgcc
      541 ggcttcatcg gccggcgcgc tcattcagaa aaaatttctt cagctggcgg ggctgcgcgc
      601 ctgcttcagc ttgttcagcg cctgcgcggg aaacgccacc ggcgcgccct gcagcgattc
      661 gaaatgcgca ccgacgtaat catcgaggtc gctggtcgcc ggtgatgctt cggcgcaact
      721 gaggccggtg ctgcgatggt cgtcgcgcac gatcggcgcc gggccagaac gatcctgctg
      781 cgccacgatg taattctgac cggccagcgc cagcgagccg cccgagagca aggccagcgc
      841 gagcagcatg cgggtagaac ggttcatgtg ggcaatatag tccaccgccc ggtgaaagtc
      901 tccctggtca accttactta acctggaccg caacgccctt gttgtcgtta taaaataact
      961 aacgatatat aattaattta atattttata acacgtaaag aagcgatgcg gttacacctg
     1021 attacgcgat ccagcggtgg gtcaggcaag tcgcagatgg tcgcggggat cgtcgaaccg
     1081 gtcctggatg gccgacagca gcggataaag cacctggttc aggtccgcgc ggcaatgact
     1141 gcaggtggat tgccggtaaa accagcgcag cgggtattcg agcggcttgc ggcaactggg
     1201 gcaatcgagc gtgatgactt gcgagaggcg atccatcggg cgtccttgtt cgcatgaggg
     1261 actgtatgca caggagaaag gttcaaggcg aattcgcagg cctttcaacg ggtcttgggg
     1321 tcgaggttcc atggaacccc accgacgggg ccgatgccgg cggaaaagtt cagccgcact
     1381 ccgggtggct gcgccctttc caataggcat gaacacgaaa atcgaaaaac tggaaagcgc
     1441 actgcggacc cgcatcgcgg gactgaagga ttggctggat cagaacggcc gcggctgccg
     1501 cgagaagcag gctcatctga ccgaggacag cgacgagcgc gcctactggc attacggcta
     1561 ctgggtggcc ttggtcgact tgctggccct gctgacggcc gaggattcct cgggctgagc
     1621 cgggttgcat acggggcagc accagtccgt gccggcgggc cgctgcggat gcaccgggtt
     1681 atccggcggc gtataggtgt gatacagccg gcactgctgc tcgaagccga tgcgtgccga
     1741 caacacgtaa cggaattcga agtgccggta gcgctgccgg tcggcgtgtt cgcgcaacgc
     1801 gcggccaaga tcgtccgcgc atccgacgaa gcggatgctg aaggtgtagc cattgtcgtt
     1861 gcccagcgca tacgcgcccg ggccagtgtg gaccacccgt tcgttgatca attccggcgt
     1921 cagccggtac ggcccgcggt aaccttcgat cggcatccct gctgtcccct tctcactcac
     1981 caagtctgag tgctaacagc gccttaaggt tcagcgggat tccgcgggaa ccagcggccc
     2041 tgtcggcgcg cctttttggg gcacgcgggg atgtacgtgt gtgctccagg tatggatgcg
     2101 cgcgtggccg ggccgttccc agtccggtgg catccacagg tgccgcagcc gctgcagcaa
     2161 ggggccgggc gagagcacgt cgcgccacat gtcgacgaac tcgtggaagt tgagcacgaa
     2221 gaaattgtac gagcgcacct ggcgcggtat gccgtaatcc accttctcca cctccggctc
     2281 gtaggtgccg aaccagcggt cgaagatgat cagcacgccg ccgtagttct tgtcgatgta
     2341 ctgcgggttg cgcccgtggt gcacccggtg gttgctcggc gtgtcgaaga tgtattcgta
     2401 ccaggccggc aatcttccga tcgcctcggt gtgcacgaag tactggtagg cgaggtccac
     2461 ggcgagcatg aacatcacca ccgccggcgg cacgcccagc cacaccggcg gcaggtagaa
     2521 gatgaagatg ccgaccacgg cgttgagcag gctctggcgc atggcggtgg tgaagttcat
     2581 cacctcgccg ctgtggtgcg gcacatgcgc gctccaccac cagcgcacgc ggtgcgacga
     2641 gcggtggaac cagtagtagc agaactccac cagcacgaag atcgggatca ccgtccagcc
     2701 attcaccggg atggtgaaca ggcggtgcga gtagacccag ccgaagatcg cgccggtgac
     2761 cagcagccac atcagcactt ccatcacctg gtagccggcg ccgagcgaca ggttggccag
     2821 gatctccttc cagtagaact gcgcgccgcg tccgcgctgc ttctccaccg cgaactcgat
     2881 catgaaggtg atcaggaaga tcggcgacat gccgatgagg aacacctgct tccagtccac
     2941 ctcggggccg aagctggcga cgatccagtc atgaatattg tggaggagct gatccatgcc
     3001 gcgattttcc gccttttgcc ctaccagcgc agcatcggca tgggcgacag cggatgcgtg
     3061 cgcaggcgct gcgcaaacag ctgcaccagc cgcttgtgaa agccggcatt gggaaaggcc
     3121 gcgaacgctt cacgcagcag cgcgtgcggc agaccgtagg cgacgagtcc atgggacacg
     3181 tcggccatgt ccgccttgcg gaacgcttcg gccagcgcgc tgctgcgata gggcgtgacc
     3241 ttgtggtgct gcacgatcat ggcctcgact tcgtcggccc agtccgcgcg gccgttgccc
     3301 tgcagccagg cgcgcgca
//
LOCUS       DASZZC010000632         4369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_747130,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000632 DASZZC010000000
VERSION     DASZZC010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747130"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VHE37_13465"
     CDS             complement(<1..1389)
                     /locus_tag="VHE37_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36591.1"
                     /translation="MVKQQAITTRSLHGLARWMVMSVLLLGLVTACARREDAVADLQQ
                     KNLLIEQLQLPAAGAEPQQLAQMTQAIKSRAESFKQLVRTYPGSARSAMLDARTLAAL
                     RAQFPQLSKFLESEGSWSGDLVPVHFDNFDARVSVDGWMLHSMDGRADLAPVDAALLR
                     ALGKQAITVSGIRIGDVIAVESALPRLDLPRAGRAKNYIATSSAAAPGDCTTVGEQDF
                     AVILMGQPDSPAFPLGAMPVSFWQQQYFSTTPRSLNSFWHETSYGQASAAGQVFGPYD
                     FDQNYSCSDSGSIYNSALAAAKAAGADLSPYSRVSIVFAENSCWYGGLGEVGCGGADE
                     NIPHPYSVNWIPVSGTGSNAWDIPRVLFHESGHNLGLSHANTLDFGSIPLGAIDYTAT
                     NPGTVTQPDTMAPDSAGGSAVNEEYADPYSAMDGSSNLHSYSAFAKVMQLGWIPFGDR
                     REVTAAGSYVLAP"
     gene            1568..2020
                     /locus_tag="VHE37_13470"
     CDS             1568..2020
                     /locus_tag="VHE37_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36592.1"
                     /translation="MLRRSIVASLLASALCSLAACVSQSVGPNGVEQSVGPFHQSIGP
                     SGVSQGMGSGGPSQNLGAGGVYQTTGSGGPSQSVSPSGVQQSSAAPQPPPKMSLSGMT
                     VTLPGASSGSSCRATCAGKQYAIACPADQRATCQCSNVPYASCVTAQH"
     gene            complement(2135..2893)
                     /locus_tag="VHE37_13475"
     CDS             complement(2135..2893)
                     /locus_tag="VHE37_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36593.1"
                     /translation="MLKRKMLLLLPLCLAAGAAAADSWSRTSDVGAHPVRHDVDDQFS
                     FAADGRIDIGNLGGSLHVAAVDQPRLTLHYERRAATQADFDCEQLRYEHSADHLTIRI
                     ERTHSEACREIRADDQLTLTVPRHASLKADSIGDSVQVSGVTGAVELSSVGDSAVLEG
                     VEQLRADSIGDHLKLSLAHLAPAGINVSSVGDSVELQLAPGIGAELDVDSVGGHVTAP
                     ALQLGSVSGGVQSRIGGGGPAIHISSVGDDVVIR"
     gene            3008..3508
                     /locus_tag="VHE37_13480"
     CDS             3008..3508
                     /locus_tag="VHE37_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36594.1"
                     /translation="MLKHLPALALRDATSAAITVALWMWTLHTGAAHDAQTVTLHLVT
                     ALFTVLAGYLVHEWGHLLGALYAHGVVHLPPTPLATFLFRFDTVRNTRAQFTAMSLGG
                     FIASVLAVALLLWLLPFQLLASRVALALVALGVIATFILEVPSAWRVHRGAPMPQGAA
                     FVSTPQ"
     gene            3542..3988
                     /locus_tag="VHE37_13485"
     CDS             3542..3988
                     /locus_tag="VHE37_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HVT36595.1"
                     /translation="MSASEFFYVLLGLAVTAATVWPSRHLYRAGTGAASLIEWTCYVT
                     ALAALVVGWYFNFQYMRAYGAQAGWWHWTTLLFVNPASASGGQDLIFGNVILFPIWTV
                     IDARRTGMGTRAGWMYFVMSIFTSFAFAFALFLAVRERQLRCNARS"
     gene            complement(3985..4293)
                     /locus_tag="VHE37_13490"
     CDS             complement(3985..4293)
                     /locus_tag="VHE37_13490"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT36596.1"
                     /translation="MSERITPQGAEKMLAGLNGQRWATMFKRGGLELEYYAPRGTDPQ
                     KPHTRVEIYFVISGSGTFVIDGKPQPFGPGEVLYLPPQVPHHFENFTDDFATWAIFAD
                     "
BASE COUNT          683 a         1511 c         1407 g          768 t
ORIGIN      
        1 cggcgccagc acgtagcttc cggccgcggt cacttcccgg cggtcgccaa acgggatcca
       61 gcccagctgc atcaccttgg caaaggcgct gtacgaatgc aggttggagc tgccgtccat
      121 cgcgctgtac gggtcggcat attcctcgtt gaccgcgctg ccgccggcgc tgtcgggcgc
      181 catggtatcc ggctgtgtca ccgtccccgg attggtcgcg gtgtagtcga tggcgcccag
      241 cgggatcgag ccgaagtcca gcgtgttggc atgcgacaag cccaggttgt gccccgactc
      301 gtggaacagc acgcgcggga tgtcccaggc gttggagccg gtgcccgaaa ccggaatcca
      361 gttcaccgag tacggatgcg ggatgttttc gtcggcgcct ccgcagccga cttcgcccag
      421 tccgccgtac cagcaggaat tctccgcaaa tacgatggac acgcggctgt acggcgacag
      481 gtccgcgcct gccgcctttg ccgcggcaag cgctgagttg tagatgctgc ctgaatccga
      541 acagctgtag ttctggtcga agtcatacgg tccgaacacc tggccggcgg cggacgcctg
      601 gccgtacgag gtctcatgcc agaaggagtt gagcgatctc ggcgtggtgg agaaatactg
      661 ctgttgccag aaactcaccg gcatggcgcc gagcgggaat gccgggctgt ccggctgtcc
      721 catcaggatc accgcaaaat cctgctcgcc caccgtggtg cagtcgcccg gtgccgctgc
      781 cgatgacgtg gcgatgtagt tttttgcgcg gcctgcgcga ggcaggtcca ggcgcggcaa
      841 cgcggattcc acggcgatca cgtcgccgat acggatgccg ctcacggtga tggcctgctt
      901 gccgagtgcg cgcagcagcg cggcgtccac cggcgccagg tcggcgcggc cgtccatgga
      961 atgcagcatc cagccatcga cgctgaccct ggcgtcgaag ttgtcgaagt gcaccggcac
     1021 caggtcgccg gaccagctgc cttcgctctc caggaatttc gacaactgcg gaaattgcgc
     1081 gcgcagcgct gcgagcgtgc gcgcgtcgag catcgccgag cgcgccgatc ccgggtacgt
     1141 ccgcaccagc tgcttgaacg attcggcgcg tgacttgatc gcctgcgtca tttgcgccag
     1201 ttgctgcggc tccgccccgg ctgccggcag ctgcaactgc tcgatcagca ggttcttctg
     1261 ctggaggtcg gcgactgcgt cctcgcggcg cgcgcatgcc gtcaccaagc ccagcagcag
     1321 caccgacatg accatccacc gtgccagccc atgcaatgag cgtgtcgtga tcgcctgctg
     1381 cttcaccatg ctgttccccg atttcgtcca gcctccctgg cggaagacca cccccggtca
     1441 ggttagccga gccatgccgg cgacaccact ggccgccgcc gctgcgacca cgtagattga
     1501 ctgcgcaccg ggcggatgcg gaacagccgc cgcccgccgg aatcaaaact caaacgggga
     1561 gtcatccatg ttgcgtcgta gcatcgttgc gtccttgctg gcctctgccc tgtgcagcct
     1621 cgccgcctgc gtctcgcaaa gcgtcgggcc caacggcgtc gagcaaagtg tgggcccgtt
     1681 ccaccagagc atcggcccgt ccggcgtgag ccagggcatg ggctcgggcg gaccttcgca
     1741 gaacctcggc gccggcggcg tgtaccagac caccggttcg ggaggacctt cacagagcgt
     1801 gagtccatcc ggcgtgcagc agtccagcgc agcgccgcaa ccgccgccga agatgtcgct
     1861 gtccggcatg accgtcacgc tgcccggcgc atcgagcggc agcagctgcc gcgccacctg
     1921 cgccggcaag caatacgcca tcgcctgccc ggccgaccag cgcgcaacgt gccaatgctc
     1981 gaacgtgccg tacgccagtt gcgtgaccgc gcagcattga ttttttgccc caccgcaccg
     2041 gcctgcagca tgttataaaa tattatttat atttatttac gatagataat taataacaag
     2101 cacacggcgg gtgcggtgga ttcatttcgc cgcgtcagcg gatcaccacg tcgtcgccga
     2161 cgctgctgat gtggatggcc ggcccgccgc cgccgatgcg gctctgcacg ccgccgctga
     2221 ccgaaccgag ctgcaatgcc ggcgcggtca cgtgaccgcc gacgctgtcc acgtcgagct
     2281 cggcgccgat gcccggcgcc agctgcagct cgaccgaatc gccgacgctg ctcacgttga
     2341 tgccggccgg agccaggtgc gcgaggctca gcttgagatg gtcgccgatc gaatccgcac
     2401 gcagctgctc cacgccttcg agcaccgcgc tgtcgcccac cgatgacagt tccaccgccc
     2461 cggtgacgcc gctcacctgc acgctgtcgc cgatcgaatc ggccttgagc gacgcgtgtc
     2521 gcggcacggt gagcgtgagc tgatcgtcgg cgcggatctc gcggcaggcc tcgctgtgcg
     2581 tgcgctcgat gcggatggtg agatggtcgg cgctgtgctc gtagcgcagc tgctcgcagt
     2641 caaagtcggc ctgcgtcgcg gcgcggcgct cgtagtgcag cgtcaaccgc ggctggtcga
     2701 ccgccgccac gtgcaggctg ccgccgaggt taccaatgtc gatgcggcca tcggcggcga
     2761 agctgaactg gtcgtcgaca tcgtggcgca ccgggtgcgc acccacgtcg ctggtgcgcg
     2821 accagctatc ggccgcagcc gcgcccgcgg cgaggcacag cggcaacagc agcagcatct
     2881 tgcgcttcaa catggaactc tcccgtgatt ggtgacgccc ttggatgcgc cggcgcgccc
     2941 gcgggtttga accgcgactg ctcccttcgg actaagcggc gcgcgggccg ccgcgccaag
     3001 atcggccatg ctcaagcacc tgcctgccct cgcgctgcgc gacgcaacca gcgccgcgat
     3061 caccgtcgca ttgtggatgt ggacactgca caccggcgct gcgcatgacg cacagaccgt
     3121 cacgctgcac ctggtcaccg cgctgttcac cgtgctggcc ggctatctcg tacacgagtg
     3181 ggggcacctg ctcggcgccc tctacgcgca tggcgtggtg cacctgccgc cgacgccgct
     3241 ggccactttc ctgttccgct tcgacaccgt gcgcaacacg cgcgcgcagt tcaccgccat
     3301 gtcgctcggc ggcttcatcg ccagcgtcct cgccgtcgcg ctgctgctgt ggctgctgcc
     3361 attccagctc ctcgcctcgc gcgtggcgct ggcgctggtg gcgctcggcg tcatcgccac
     3421 cttcatcctc gaagtgccgt cggcctggcg cgtgcatcgc ggcgcgccga tgccgcaggg
     3481 agccgcgttc gtctcgacac cgcagtagtc cgcacaacga cacaacaaca agggggtcag
     3541 cttgagcgca tcggaattct tctacgtgct gctgggcctg gccgtcaccg ccgccaccgt
     3601 gtggccgagc cgccatctct atcgcgccgg caccggcgcg gcgtcgctga ttgaatggac
     3661 ctgttatgtc accgcgctgg ccgcgctggt ggtcggctgg tacttcaact tccagtacat
     3721 gcgcgcttac ggcgcgcagg ccggctggtg gcactggacc acgctgctgt tcgtcaaccc
     3781 ggcctcggca tccggcggac aggacctgat cttcggcaac gtcatcctgt tcccgatctg
     3841 gacggtgatc gacgcgcgcc gtaccggcat gggcacgcgg gcaggctgga tgtacttcgt
     3901 catgagcatc ttcaccagct tcgccttcgc cttcgcgctg ttcctcgcgg tgcgagagcg
     3961 gcaactgcgc tgcaacgcca ggagctagtc ggcgaagatc gcccaggtgg cgaaatcgtc
     4021 ggtgaagttc tcgaagtggt gcggcacctg cggcggcagg tacagcacct cgccggggcc
     4081 gaaaggttgc ggcttgccgt cgatgacgaa cgtgccgctg ccggagatga cgaagtagat
     4141 ctccacgcgg gtgtgcggct tctgcggatc ggtgccgcga ggcgcgtagt actccagctc
     4201 caggccaccg cgcttgaaca tggtggccca gcgctggccg ttcaggcccg ccagcatttt
     4261 ttccgcgccc tgcggcgtga tgcgttcgct catgcttttt ctccgaggtg atgcggctac
     4321 gctagcgcag gcgcgcgccg cgtgaaagcc aaccacaagt tcgatgaca
//
LOCUS       DASZZC010000633         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1147091,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000633 DASZZC010000000
VERSION     DASZZC010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147091"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VHE37_13495"
     CDS             <1..368
                     /locus_tag="VHE37_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36597.1"
                     /translation="VGGAVGLVAGAVVGAAAGHAIGEKVNPTQEAEYWRSNYTTEPYY
                     SSMYTYEDYDPAYRVGYEGYARYQGRSFDEVERDLEVDYNRAKGKSRLSWEHAKQATR
                     RAWDRLTGHRQDDDLSSSH"
     gene            435..632
                     /locus_tag="VHE37_13500"
     CDS             435..632
                     /locus_tag="VHE37_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36598.1"
                     /translation="MNKRKQDFVKDKEDQGAPAYYSGGAQRGGNGKSQYANERNASSS
                     SRGPARPRPEQDDALSSRGSA"
     gene            complement(639..1451)
                     /locus_tag="VHE37_13505"
     CDS             complement(639..1451)
                     /locus_tag="VHE37_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVT36599.1"
                     /translation="MKKSAGKSGAIRAGIGGWDYEPWRKTFYPAGLPQKRALEYASRQ
                     LSAIEVNGTFYRTQQPSTFKRWHDETPDGFVFALKAVRYAVNRRVLAEAGPSIKLFID
                     SGIAELGAKLGPILWQLAPTKKYDADDIARFLDLLPEKAGGVPLRHVLEARHESFLVP
                     EFIGQVRGRGIATVYTDSAEYPSFADVSAGFVYARLMCAQASLKTGYAPKDLKAWAQR
                     AQTWAQGGWPADLHCVVQKAPVKTPRDVFVFFINGAKERAPAAALGLIAALQ"
     gene            complement(1461..>2453)
                     /locus_tag="VHE37_13510"
     CDS             complement(1461..>2453)
                     /locus_tag="VHE37_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVT36600.1"
                     /translation="LVNWLRQRARLPVPLGAALVLTLFVGGILAGTNALMPQATRLLD
                     AVPRVTHNLERVLNRTALSQSSAIRKATVAAGELQKVANTAVGATPAPNAATPDTSSS
                     EIDLRERLLAGGAAAMRGLGTLVVVIALIYFLLISSHAFKRKLVKISGHTLRQKKLTV
                     QILDDIDQQMQLYLLIQLSTSALVGVATGIVFGAIGLENAAFWGAAAGVLHLVPYVGP
                     GFIVIASAMFAYLQFDKLETVLLVSGSALAIAGVVGLGLVPWLTGRLARLNAVSTFIA
                     LLFWDWLWGIPGLLLGIPIMMALMAVCERVDQLVPVAELLSSDAPRKTRASAAA"
BASE COUNT          410 a          837 c          844 g          362 t
ORIGIN      
        1 cggtcggtgg cgccgtcggc ctggtggcgg gcgcagtggt cggcgcggcc gcgggccatg
       61 ccatcggcga gaaggtaaac ccgacgcagg aagccgagta ctggcgcagc aattacacca
      121 ccgagccgta ctacagcagc atgtacacct acgaggacta tgacccggcg taccgcgtcg
      181 gctacgaagg ctacgcgcgg tatcaaggtc gcagcttcga tgaggtcgag cgtgatctcg
      241 aggtggacta caaccgtgcc aagggcaagt cgcgcctgag ctgggaacac gccaagcagg
      301 ccacgcgccg tgcgtgggac cggctgaccg gccatcgcca ggatgatgac ctgtccagct
      361 cgcactgaat gcggatgcgc agcgcggcgc gcttggagtg cgcgccgcgc ctgcgctccc
      421 cttgaaggca agccatgaac aagcgcaagc aggatttcgt caaggacaag gaagaccagg
      481 gcgcgcccgc ctattattcc ggcggggcgc agcgcggcgg caacggcaag tcgcagtatg
      541 ccaatgagcg caatgcctct tcatcctcgc gtggaccggc caggccgcgg cccgaacagg
      601 acgacgcgct gagttcgcgc ggtagcgcct gaagcccgct actgcagcgc ggcgatgagc
      661 ccgagcgcag ccgccggcgc gcgctccttg gccccattga tgaaaaacac gaagacgtcg
      721 cgcggggttt tcaccggtgc cttctgcacc acgcaatgca ggtcggcggg ccatccgccc
      781 tgcgcccacg tctgcgcgcg ctgcgcccac gccttgagat ccttcggcgc gtagccggtc
      841 ttgagcgacg cctgcgcgca catcagccgc gcatagacga agccggcgct cacatcggcg
      901 aacgacggat attccgccga atcggtgtac acggtggcga tgccgcgccc gcgcacctgc
      961 ccgatgaatt ccggcacgag aaagctctcg tggcgcgcct cgagcacgtg gcgcagcggc
     1021 acgccgccgg ccttctccgg cagcaggtcg agaaaacgcg cgatgtcgtc ggcgtcgtac
     1081 ttcttggtcg gcgccagctg ccacaggatg gggccgagct tggcgccgag ttcggcgatg
     1141 ccgctgtcga tgaacagctt gatcgacggc cccgcctccg ccagcacgcg gcggttcacc
     1201 gcgtagcgca ccgctttcag cgcgaagacg aagccgtccg gcgtctcgtc gtgccagcgc
     1261 ttgaaggtgg acggctgctg cgtgcggtag aacgtgccgt tgacctcgat cgcgctgagc
     1321 tggcggctgg cgtattccag cgcgcgcttc tgcggcaggc ccgccgggta gaacgtcttg
     1381 cgccagggct catagtccca gccgccgatg ccggcgcgga tggcgccgga cttgcccgcc
     1441 gattttttca tgcggcgagt ttaggccgcg gcacttgcgc gtgtcttgcg cggcgcgtcg
     1501 ctcgacaaca gttcggccac cggcaccagc tggtcgacgc gctcgcacac cgccatcagc
     1561 gccatcatga tcggaatgcc cagcagcagc ccggggatgc cccacagcca gtcccagaac
     1621 agcagcgcga tgaacgtgct caccgcgttg agccgcgcga gacggccggt gagccacggc
     1681 accagcccca gtccgaccac gccggcgatg gccagcgcgc tgcccgacac cagcagcacc
     1741 gtttccagct tgtcgaactg caggtaggcg aacatggcgg aggcgatgac gatgaagccc
     1801 ggccccacgt acgggaccag gtgcagcacg ccggccgccg cgccccagaa ggccgcgttc
     1861 tccagcccga tggcgccgaa cacgatgccg gtggcgacgc ccaccagcgc gctggtcgac
     1921 agctggatca gcaggtacag ctgcatctgc tggtcgatgt cgtcgaggat ctgcacggtg
     1981 agtttcttct ggcgcagcgt gtgcccgctg atcttcacca gcttgcgctt gaacgcgtgg
     2041 ctggagatga gcaggaaata gatcagcgcg atcaccacca ccagggtgcc gagcccgcgc
     2101 atggccgcgg cgccgccggc cagcaggcgt tcgcgcaggt cgatctcgga gcttgatgtg
     2161 tcgggcgtgg ccgcgttcgg cgcgggtgtg gcgcccaccg cggtattggc cactttctgc
     2221 agttcgccgg cggccaccgt cgccttgcgg atggcgctgg actgcgacag cgcggtgcgg
     2281 ttgagcacgc gctccaggtt gtgggtgacg cgcggcaccg cgtcgagcag gcgcgtggcc
     2341 tgcggcatca gcgcgttggt gccggccagg atgccgccga cgaacaatgt cagcaccagc
     2401 gccgcgccca gcggcaccgg caggcgcgcg cgctggcgca gccagttgac cag
//
LOCUS       DASZZC010000634         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_688282,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000634 DASZZC010000000
VERSION     DASZZC010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688282"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VHE37_13515"
     CDS             <1..848
                     /locus_tag="VHE37_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HVT36601.1"
                     /translation="KALEQQLEQAKRRFDVGLSAITDVQDAQARFDATAANVIAGQQA
                     YDAANEALALITGHPFDHLASLQEDIPLPAPDPSDINAWVSAANENNYGLLQARLNSD
                     IADKGVGIARGGHYPSLGLSGREVRSLQGGFNTAHVDSSSVTLSLNVPIFAGGKTQAL
                     VRQAEATHEQRQAELELARRTAGQSTRNAYQGVVAGAARVKALKQAVVSNTTAVEASK
                     TGLEVGTRTQVDVLTAEQLLYAAQRDYYKSRYDYLVAVLQLKAAAGRLTEADLEQIDK
                     LLVSG"
     gene            862..1836
                     /locus_tag="VHE37_13520"
     CDS             862..1836
                     /locus_tag="VHE37_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVT36602.1"
                     /translation="MVVFVTGGSGFVGRNLIRACIARGDRVLALARSPQARQQVEQLG
                     AQAVAADLDSVTAAQLAGVQCVIHAAAKVSEWGARDEFRSVNVDGTRHLLEVARTAGV
                     SSFVLIGTEAAYADGNPIIDVDETRPLPAHPLPRYPATKSEADRMVRAANAPGFRTVV
                     VRPRLIWGRDDTSVLPQLIEAVKKGQFAWVNGGRYLTSTCHVANVCEGALLAAEHGRG
                     GEAYFLTDGAPVEFRDFMTRLFATRGMGAPDRTIPFGVAKLFAATCEKLWEWLPLPGH
                     PPLTRMAINLGAQQVTVRDAKARRELGYRGQVSIEQGLAELASNRFGI"
     gene            1916..>3001
                     /locus_tag="VHE37_13525"
     CDS             1916..>3001
                     /locus_tag="VHE37_13525"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HVT36603.1"
                     /translation="MQLGAWIYRGVAGASLTAFAAAAAAQDATTQDLEQRLRVVERQL
                     EIQKEEAEARAKDSSQAVAGDKGFSLKSADGSYALNFKALVQADGRFYTGDPAAQGIN
                     DGFLLRRVEPTFQASLGKLVGVTFTPNFSAGSNSSPGVAVSDLFVEVKFDPAYTLRIG
                     RYKTPVSGLENLQPSGAIRFIERGLPTSLAPNRDFGAQLQGALFGSTLSYAVGIFNGA
                     ADGRDAVPSDTDNRKEIAARIFAEPFKNSPGFFQGLGFGIAGSRGTKLGANTAVNGVN
                     SVYQGYVTTGQQTFFNYAPSAPAGSATTVAFAGLETRIQPQLYFYRNSFGLLAEYSTS
                     RQELTATNVAGAVTTVTGHDALTHKAYQ"
BASE COUNT          553 a         1020 c          948 g          480 t
ORIGIN      
        1 acaaggcgct tgagcagcaa ctggaacagg ccaagcgccg cttcgacgtg gggctttccg
       61 ccatcaccga cgtgcaggat gcgcaggcac gcttcgacgc caccgccgca aatgtcattg
      121 ccggccagca ggcttatgac gccgccaacg aggcactggc cttgatcacc ggccatccct
      181 tcgatcatct ggcctcgctg caggaagaca ttcccctgcc cgcgcccgac ccgagcgata
      241 tcaatgcctg ggtcagcgcg gcgaacgaga acaactacgg gctgctgcaa gccaggctta
      301 attccgacat tgccgacaag ggcgtgggca tcgcgcgcgg tgggcactat ccttcgctcg
      361 gactctcggg ccgcgaagtg cgcagcttgc aaggtggttt caacacggcg cacgtcgatt
      421 cttccagcgt cacgctgtcg ctcaacgtgc cgatctttgc cggcggcaag acccaggctc
      481 tcgtgcgcca ggccgaggcc acgcatgagc agcgccaggc agaactggag cttgcccggc
      541 gtaccgccgg gcagagcacc cgcaacgcct accagggcgt cgttgccggc gcagcgcgag
      601 tcaaggcgct gaagcaagcg gtggtttcca ataccaccgc agtggaggcc agcaagaccg
      661 ggctcgaagt tggcacgcgc acgcaggtcg acgtgttgac cgcggagcaa ttgctttatg
      721 ccgcccagcg cgactactac aaatcgcgct acgactacct cgtcgccgtg ctgcagctga
      781 aagccgccgc cggccggctg accgaggccg acctggagca gatcgacaag ctgctggtca
      841 gcggctgagc tacggcggcc cgtggtcgtc ttcgtcaccg gcggctcggg cttcgtcgga
      901 cgcaacctga tccgcgcctg catcgcgcgc ggcgaccgcg ttctcgcgct ggcacgcagc
      961 ccgcaggcgc gccaacaggt tgagcaactc ggcgcccagg ccgtagctgc cgatctcgac
     1021 agcgtgacgg ccgcgcaact ggccggtgtg caatgcgtga tccacgccgc cgccaaggtc
     1081 agcgaatggg gcgcacgcga cgagtttcgg agcgtcaacg tcgacggcac gcggcacctg
     1141 ctcgaggtgg cgcgcaccgc gggcgtaagc agcttcgtgc tgatcggcac cgaagctgcg
     1201 tatgccgacg gcaatccaat catcgatgtc gatgaaacgc ggccgctgcc ggcacatccc
     1261 ctgccgcgct accctgctac gaaatccgaa gcagaccgca tggtgcgcgc ggccaacgct
     1321 ccaggcttca ggaccgtggt ggtgcgcccg cgactaatct ggggccgcga cgacacctcg
     1381 gtgctaccgc aactgatcga agctgtgaag aaaggacagt tcgcttgggt caacggtggc
     1441 cgttacctca cctcgacctg ccatgttgcc aacgtatgcg aaggtgcgct gcttgcggcc
     1501 gaacacgggc gcggcggcga agcatatttc ctcaccgacg gtgcaccggt ggaattccgt
     1561 gatttcatga cgcggttgtt cgccacgcgc ggcatgggcg cgcccgacag gaccattccc
     1621 ttcggcgtgg cgaagctctt tgcggcgacc tgcgaaaagc tgtgggaatg gctgccgctg
     1681 cccggtcacc cgccgctcac gcgcatggcg atcaacctcg gcgcgcagca ggtgaccgtg
     1741 cgcgacgcca aggcgcgccg cgagctggga taccgcggcc aggtcagcat cgagcaggga
     1801 ctggccgaac tggcctctaa tcgttttgga atataaacct aacttaatat tctttttaaa
     1861 ctatcacgcg ctcgcgcaag atgcgccacc tcaatcaaca acgggagtgg cggcaatgca
     1921 attgggagct tggatttacc ggggcgtcgc gggcgcatcg ctgaccgctt tcgccgcagc
     1981 cgcggcggcg caggacgcga ccacgcagga cctggaacag cggctgcgtg tcgtcgagcg
     2041 ccagctggaa atccagaagg aagaagccga ggccagggcc aaggacagtt cgcaggctgt
     2101 ggccggcgac aagggcttct cgctcaagtc ggctgacggc agctatgcgc tgaacttcaa
     2161 ggcgctggtg caggccgacg gccgcttcta caccggcgat cccgccgcgc agggcatcaa
     2221 cgacggcttc ctgctgcgcc gcgtggaacc cactttccag gcttcgctgg gcaagctggt
     2281 aggcgtgact ttcacgccga acttcagcgc cggcagcaac tcctcgccgg gcgttgccgt
     2341 cagcgacctg ttcgtcgaag ttaaattcga ccctgcctac acgctgcgca tcggccgcta
     2401 caagacgccg gtctccggtc tggaaaacct gcagccctcc ggtgccatcc gcttcatcga
     2461 gcgcgggctg cccaccagcc tggcgcccaa ccgcgacttc ggcgcgcagc tgcagggggc
     2521 gctcttcggc agcacgctga gctacgcggt gggcatcttc aacggcgccg ccgatggccg
     2581 cgacgccgtg cccagcgaca ccgacaaccg caaggaaatc gccgcgcgca tttttgccga
     2641 gccgttcaag aattcgcccg gcttcttcca gggcctgggc ttcggcatcg ccggcagccg
     2701 cggcaccaag ctcggcgcca acaccgcggt caacggcgtg aacagcgttt accagggtta
     2761 cgtcaccacc ggccagcaga cgttcttcaa ctacgcgccg tcggctcccg cgggttccgc
     2821 cacgaccgtg gcgttcgccg gcctcgagac gcgcatccag ccgcagctgt atttctaccg
     2881 caacagcttc ggcctgctgg cggaatacag cacttcacgc caggaattga ccgcgaccaa
     2941 cgtggcaggt gcggtcacta cggtgacggg acatgatgcg ctgacacaca aggcctacca
     3001 g
//
LOCUS       DASZZC010000635         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_874801,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000635 DASZZC010000000
VERSION     DASZZC010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874801"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VHE37_13530"
     CDS             complement(<1..1280)
                     /locus_tag="VHE37_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVT36604.1"
                     /translation="MTNALLAPHAHSPLPPFDRITPADALPALEQVLADNRAALAALL
                     SQDAAPAWSTLIEPLEDMGDKLSRAWGPTQHLFGVMSTKAWREAYNGGLPKITEYSLE
                     LAQSEPLYRAYLALRESPAHAALTPARKKVIGDALRDFKLSGIALPEAQKQRFKDISL
                     RLSELQAKFEENLMDAIQAWSKQVSDASLLAGMLPEAREAAAAKAKAKNLDGFLLTLD
                     FPSYDAVITYADNRELRRELYEAYATRASDRGPLAGQHDNSPLMAEILALRHEEAQLL
                     GFHNFAELSLATKMAESPAEVETFLLDLARRARAAAQGELDELKDFSGLPDFQPWDAA
                     YYAEKLREKKLGLSDEELKPYFPLPQVLSGMFELVSKLYGVRIEAAPGIPVWHADVTT
                     YALLDERGERFGLFYLDPYARDDKRSGAWMDECV"
     gene            complement(1402..>2453)
                     /locus_tag="VHE37_13535"
     CDS             complement(1402..>2453)
                     /locus_tag="VHE37_13535"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HVT36605.1"
                     /translation="MRTRADPYQALFMVGLAGAVASFLHDFPGIARAAGTLPALMPWL
                     VSAAAALLVLRGMVLLRRARMIEDTPLSLARSAAQGYVELEGHARLLPGPEIISPLSR
                     QRCTWWSYRVWQRRATVNGGNEWVLLEDQTSDDLFQLADTSGEVIIDPYGAKVLPSVD
                     RSWRGRRPRPDMVPERSEWIGFGEYRYRERLLRVGDALYAAGEFRTQAAIAEFNESRD
                     VTELLREWKKNQRELLRRFDTNHNGIIEPAEWEAARQAAQAQVRAQQLERATQPDLSI
                     LTHPSDGRPFLLSVRPQAQLVAQMRRRALLALLLAIAIGGGTDWVLRAAQGCAQASPH
                     PGPACFIISNIFKNK"
BASE COUNT          373 a          811 c          852 g          417 t
ORIGIN      
        1 ttgacgcatt cgtccatcca cgcgccgctg cgcttgtcgt cgcgcgcgta cgggtcgagg
       61 tagaaaaggc cgaagcgctc gccgcgctca tcgagcagcg cgtaggtggt gacgtcggca
      121 tgccacaccg ggatgccggg cgccgcctcg atgcgcacgc cgtagagctt gctcaccagc
      181 tcgaacatgc cggagagcac ttgcggcagc gggaaatagg gtttgagttc ctcgtcggac
      241 aggccgagct tcttctcgcg cagtttctcc gcgtagtagg cggcatccca cggctggaag
      301 tccggcaggc cggagaagtc tttcagctca tcgagctcgc cctgcgcggc ggcgcgcgcg
      361 cggcgcgcaa gatcgagcag gaaggtttcc acttccgccg gcgactcggc catcttggtg
      421 gccagcgaca gttcggcgaa attgtggaag ccgagcagct gcgcctcctc gtggcgcagc
      481 gcgaggatct ccgccatcag cggcgagttg tcgtgctggc ccgcgagcgg gccgcggtcg
      541 gacgcgcgcg tggcataggc ttcgtacagt tcgcggcgca gttcgcggtt gtcggcgtag
      601 gtgatcaccg cgtcgtagct gggaaaatcc agcgtgagca ggaagccgtc gagatttttt
      661 gccttggctt tcgcggccgc ggcctcgcgc gcttccggca gcatgccggc gagcaggctg
      721 gcgtcggaca cctgtttgct ccaggcctgg atggcgtcca tcaggttttc ctcgaacttc
      781 gcctgcagtt ccgacaggcg cagcgagatg tccttgaagc gctgcttctg cgcctccggc
      841 agcgcaatgc ccgacagctt gaagtcgcgc agcgcatcgc cgatgacttt cttccgcgcc
      901 ggcgtcagcg cggcatgtgc cggcgactcg cgcagcgcga gataggcgcg gtacagcggc
      961 tccgactgcg ccagctcgag cgagtactcg gtgatcttcg gcaggccgcc gttgtacgcc
     1021 tcgcgccagg ccttggtgct catcacgccg aacaggtgct gcgtcggacc ccaggcgcgc
     1081 gacagcttgt cgcccatgtc ttccagcggt tcgatcagcg tgctccaggc cggcgccgcg
     1141 tcctgtgaga gcagcgcggc cagcgcggcg cggttgtcgg ccagcacctg ctccagcgcg
     1201 ggcagcgcgt cggcaggcgt gatgcgatcg aaggggggca gcggcgagtg cgcgtgcggt
     1261 gcgagcaggg cgttggtcat ggaggagcgt cgggaaacgg gaatgtcgat tggacaacgg
     1321 gtgcggatat tttctcgcga aagccgcccg ccggtttgct ggaggcgccc accgcacccg
     1381 cttttcgtaa gttataaaat attatttatt tttaaaaata ttagatatta taaaacatgc
     1441 tggaccaggg tgcggtgagg cctgcgcgca gccctgcgcg gcgcgcagca cccagtcggt
     1501 gccgccgccg atggcaatgg ccagcaacag cgcaagcagc gcgcggcgcc gcatctgtgc
     1561 caccagctgc gcctgcgggc gcaccgacag caggaacggc cggccatcgc tgggatgggt
     1621 gaggatggaa agatccggct gggtggcgcg ctccagctgc tgcgcgcgca cctgcgcctg
     1681 cgccgcctgg cgcgcggctt cccattcggc cggctcgatg atgccgttgt ggttggtgtc
     1741 gaagcgccgc agcagttcgc gctggttctt cttccactcg cgcagcagtt cggtcacgtc
     1801 gcgcgactcg ttgaactcgg cgatggcggc ctgcgtgcgg aactcgccgg cggcgtacag
     1861 cgcatcgccc acgcgcagca gtcgctcccg gtaacgatat tcgccgaagc cgatccattc
     1921 gctgcgctcg ggcaccatgt cgggccgcgg cctgcgtccg cgccagctgc ggtccacgct
     1981 gggcagcacc ttggcgccgt acgggtcgat gatcacctcg ccgctggtgt ccgccaactg
     2041 gaacaggtcg tcgctggtct ggtcttccag cagcacccat tcgttgccgc cgttgaccgt
     2101 ggcgcgacgc tgccacacgc ggtagctcca ccaggtgcag cgctggcgcg acagcgggct
     2161 gatgatctcc gggccgggca gcaggcgcgc gtggccttcc agctccacgt agccctgcgc
     2221 ggccgagcgc gcgagcgaca gcggcgtgtc ttcgatcatc cgcgcgcggc gcagcagcac
     2281 catgccgcgc agcaccagca atgcggcggc cgcgctgacc agccacggca tcagcgccgg
     2341 cagcgtgccg gccgcgcgcg caatgccggg aaagtcgtgc aggaagctgg cgaccgcgcc
     2401 ggccaatccg accatgaaca gcgcctggta cggatcggcg cgcgtgcgca tgc
//
LOCUS       DASZZC010000636         6931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1018947,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000636 DASZZC010000000
VERSION     DASZZC010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018947"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1114
                     /locus_tag="VHE37_13540"
     CDS             254..1114
                     /locus_tag="VHE37_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36606.1"
                     /translation="MSEKSLLKRATLAEACDAMVLPPSGRRALFVLAGHPGRARANCA
                     LAEPADFSINVQVSGEHRQYLPDPIAFRLMPGEGSALVDLLELALFRFDTPQHDYFRV
                     YFGCRDLNELQQIEAQLTGLAFDAASGLGDWRLSNVLEWCAVERLAAAGELQPDAGVK
                     LPRPLKWSLQRPVFREGQDSPLYLWKLTSPAQPDEQRALLSCVTDHQGVVARKADLVP
                     IQRLFAPPKPPPAPKPPLQDFSRDAAPAAVKSEVEPAAPRARRALSARQLVEQAEQKE
                     KIRKLFEMSQ"
     gene            complement(1115..1486)
                     /locus_tag="VHE37_13545"
     CDS             complement(1115..1486)
                     /locus_tag="VHE37_13545"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HVT36607.1"
                     /translation="MSAIGKCRWAIAEGYIPPGGSGGTRELESHETLCILNAGERAAQ
                     IAITLYFADRAPVGPYRFEIGAQRTRHLRFNDFADPQPVPRGTDFSSVVESDVPVVVQ
                     HTRLDSRQAALALLSTIAYAE"
     gene            complement(1494..2462)
                     /locus_tag="VHE37_13550"
     CDS             complement(1494..2462)
                     /locus_tag="VHE37_13550"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HVT36608.1"
                     /translation="MATRQRSSGADLAIVGLGRMGGNLARQALARGLRVAGYTQGGKT
                     DLRHRRFTLAAQLEQLPPLLKRPRKIFLYVPAGAAVDQLAAQLAPLLQPGDILLDGGN
                     SYWGDSLRRAQRLQASGIHWLDLGTSGGVDGARHGACFMIGGPRAAVAQVAPLLRRLA
                     VPGGYVHAGGSGAGHFTKLVHNGIEFGMLQAIGEGIELLQRYGAPLPLADIVECWRHG
                     SVIRSWLIDLLAQGLRDGELRAIPPVVEDTGEVNWLVADALEMDVPIPVISQAVTQLV
                     ASREREPAAPRAVALMRHGFGGHPYGRSPALQRERRTSRIGGYLAP"
     gene            complement(2493..3491)
                     /locus_tag="VHE37_13555"
     CDS             complement(2493..3491)
                     /locus_tag="VHE37_13555"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HVT36609.1"
                     /translation="MNSISLTRFLIEEERTRHYIGGDLRLLIEVVARACKRISIALGK
                     GALGGVLGDAGTGNIQGEAQKKLDVLSNEILTEANSWGGHLAGLASEEMDTVQKIPDD
                     YPKGGYLLLFDPLDGSSNIDVNISVGTIFSVLRNPEGGDGGDEQCYLQRGREQVAAGY
                     CVYGPTTTLVLTFGHGVHVFTLDREIGGFVLTQRNLTMPADTKEFSINMSNQRFWEAP
                     VQRYISECVAGKTGPRGKDFNMRWVASMVADVHRILTRGGIFMYPLDEKTRDKGGKLR
                     LMYEANPMSFIVEQAGGASSTGRERILDIAPQKLHQRVPVIMGSKNEVERVVSYHG"
     gene            complement(3616..3999)
                     /gene="apaG"
                     /locus_tag="VHE37_13560"
     CDS             complement(3616..3999)
                     /gene="apaG"
                     /locus_tag="VHE37_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HVT36610.1"
                     /translation="MSDTTTRGIRIMVTPRYIAEQSDAHVGRYLFAYHIVIQNNSPDT
                     VQLLSRHWVITNGEGKTEEVRGPGVVGEQPVLKPGDEFEYTSGCPLTTPVGTMHGEFN
                     MVADDGEKFDARIDPFRLAVPGMLN"
     gene            complement(4146..4691)
                     /gene="sufT"
                     /locus_tag="VHE37_13565"
     CDS             complement(4146..4691)
                     /gene="sufT"
                     /locus_tag="VHE37_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HVT36611.1"
                     /translation="MSLHEPVTFARDVIGVLIPAGTKVELPEGAKGVITQALGGSFTV
                     QVEGHLFRIDGKDADAIGKTVQAGPTVADDASDADVEKAVWAQLKTCYDPEIPVDIVE
                     LGLIYECKVETLEPGKRRVTVRMTLTAPGCGMGDILVNDIKGKVSQLPSVSETDVQLV
                     FEPPWSQERMSEAARLATGMF"
     gene            4789..>6931
                     /locus_tag="VHE37_13570"
     CDS             4789..>6931
                     /locus_tag="VHE37_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT36612.1"
                     /translation="MRLTSFSLENPAAVAAAALIVLLFGGIALTKLPIQLLPDTRQPQ
                     LFVSANWREAAPNEIEEALINPIEEALKGMPGLVEMRSSAARGQSGVNLKFEVGTDMT
                     RVMLDVVNRLNTLPPLPPDADRPQVFGGDNNFTGNVAASMLVRPLPGNHTPDLAVRYQ
                     KLMEEVVQPRLARIPGVSRVNLEGGRPREVRVEFDPHKLAAAGLTPQQLAAAVAGARD
                     ASAGFVDVGRRQFTVRFAGREPVEELGNLVVGWSNERPLYLRDVADVHIAWQDAQAIT
                     YRNGVPGYYITLNRTSSSNTVELLDGAKAAIAELNEGPLAKENLVMDLSWDASVYVRR
                     AISFVQESLIIGVLLAVTGLWYFLRGPRSLVVIAASIPISLFFAVITLHALGRTLNVI
                     SLAGLAFSTGIVTDAALIVQGNIIRYVQEGRSAREATLHGALEVLPALLASMLTSVAI
                     FLPILFVQGLEGQLFRDLAITMSVSHAASLLVAMTVLPAASRWALAQKLPSDAHQHWW
                     KAMTRLSMRLTDRPQARWAWIATLVAGAVLAIDVMAPQVDYLPVAQTDNIFSNFRLPP
                     GGNLITLQKEFAPVVIARMQPYLEGKKEPAVKYYNLSAFGGQFGLMVAYAKNPRDIQK
                     LAKTLREEILVGLPDVSVYVQRGSLLDVDGGNSRDIELDFQGPDLDGLMKAAGAAMKA
                     IPQAIPGTYPQAFPSLELNQPEITLAPDEWRI"
BASE COUNT         1069 a         2351 c         2370 g         1141 t
ORIGIN      
        1 cctgcagggc tggtattacc agcagattgc agcgatggcc gacggcgcgc gcgtgccgct
       61 gcggcgcggc ctgctgcacg ccatgctggc ctacctgtgg gacgagtacc gcaccgccgc
      121 ccgcgtgcgg cgtgcgtggg gggcgcgtcg gcgctgaggg attttccttg aggtgtttcc
      181 ctcatggcgc ggacacgcgc cgtggctaag ctgcctgccc gatccatcac tcgcggtatc
      241 acgggctgca cgcatgtccg agaaatccct gctcaagcgc gccacgctgg ccgaagcctg
      301 cgacgccatg gtgctgccgc ccagcggccg ccgtgcgctg ttcgtgctgg ccgggcatcc
      361 cggccgcgcg cgcgccaact gcgcgctggc cgagccggcg gatttctcca tcaacgtgca
      421 ggtcagcggc gagcaccggc agtacctgcc ggacccgatc gcgttccggc tgatgccggg
      481 cgagggctcg gcgctggtcg acctgctcga gctcgcgctg ttccgcttcg acacgccgca
      541 gcacgattac ttccgcgtgt acttcggctg ccgcgacctg aacgaactgc agcagatcga
      601 ggcgcagctc accgggctgg cgttcgacgc cgcctcgggc ctgggcgact ggcgcctgag
      661 caacgtgctg gagtggtgcg ccgtggagcg cctcgcggcg gccggcgaac tgcagcccga
      721 cgccggcgtg aaattgccca ggcccttgaa gtggagcctg cagcgcccgg tgttccgcga
      781 gggccaggac agcccgctgt acctgtggaa gctgacctcg ccggcgcagc cggacgagca
      841 gcgcgcgctg ctgtcctgcg tgaccgatca ccagggcgtg gtggcgcgca aggccgacct
      901 ggtgccgatc cagcgcctgt tcgcgccgcc caagccgccg ccagctccga aaccaccgct
      961 gcaggatttc tcgcgcgatg ccgcgccggc tgccgtcaag agcgaggtgg aaccggccgc
     1021 accgcgtgcg cggcgcgcac tcagcgcgcg ccagctggtg gagcaggccg agcagaagga
     1081 aaaaatccgc aagctgttcg aaatgtcgca gtagtcattc cgcgtaggcg atggtgctga
     1141 gcagcgccag cgccgcctgg cgcgaatcca gccgcgtgtg ctgcaccacc accggcacgt
     1201 cgctctccac caccgaggag aaatcggtgc cgcgcggcac cggttgcgga tcggcaaaat
     1261 cgttgaagcg caggtgccgc gtgcgctgtg cgccaatttc gaagcgatac ggccccaccg
     1321 gcgcgcggtc agcgaaatac agggtgatgg cgatctgcgc cgcgcgctcg ccggcgttga
     1381 ggatgcacag cgtctcgtgg ctttccagct cgcgcgtgcc gccgctgccg ccgggcggaa
     1441 tgtagccttc ggcaatcgcc cagcggcatt tgccgatggc actcatggcg cgctcatggt
     1501 gcgaggtagc cgccgatgcg gctggtgcgg cgctcgcgct gcagcgccgg cgagcggccg
     1561 tagggatgtc cgccgaagcc gtgccgcatc agcgccaccg cgcgcggcgc cgcgggttcg
     1621 cgctcgcgcg aggccaccag ctgcgttacc gcctggctga tcaccgggat cggcacgtcc
     1681 atctccagcg cgtcggccac cagccagttg acctcgccgg tgtcttccac caccggcggg
     1741 atggcacgca gctcgccgtc gcgcaggccc tgcgccagca ggtcgatgag ccacgagcgg
     1801 atcaccgagc cgtggcgcca gcactcgacg atgtcggcca gcggcagcgg cgcgccgtag
     1861 cgctgcagca gctcgatgcc ttcgccgatg gcctgcagca tgccgaactc gatgccgttg
     1921 tggaccagct tggtgaaatg cccggcgccg ctgccgccgg cgtgcacgta gccgccgggc
     1981 acggcgagcc tgcgcagcag gggcgccacc tgcgccaccg ccgcgcgcgg gccgccgatc
     2041 atgaagcagg cgccgtggcg cgcgccgtcg acgccaccgg aggtgcccag gtccagccag
     2101 tggatgccgc tggcctgcag gcgctgcgcg cggcgcagcg agtcgcccca gtaggaattg
     2161 ccgccgtcga gcaggatgtc gccgggctgc agcagcggcg ccagttgcgc ggccagctgg
     2221 tccaccgccg cgccggcggg cacgtagaga aagattttcc gcggacgctt gagcagcggg
     2281 ggcaactgct ccagctgcgc ggcgagggtg aagcggcggt ggcgcagatc ggtcttgccg
     2341 ccctgcgtat agccggcgac gcgcaggccg cgtgccagcg cctggcgcgc gaggttgccg
     2401 cccatgcggc ccaggccgac gatggccagg tccgcgccgc ttgatcgttg ccgggtcgcc
     2461 atgtggatgc gaccgcggcc atgcgcgcgg gttcagccgt ggtaggaaac cacgcgctcc
     2521 acttcgttct tcgagcccat gatcactggc acgcgctggt gcagtttctg cggcgcgatg
     2581 tcgaggatgc gctcgcgccc cgtgctggag gcgccgccgg cctgctcgac gatgaagctc
     2641 atcggattgg cctcgtacat caggcgcagc ttgccgccct tgtcgcgcgt cttctcgtcc
     2701 agcgggtaca tgaagatgcc gccgcgggtg aggatgcggt gcacgtccgc caccatcgac
     2761 gccacccagc gcatgttgaa gtccttgccg cgcgggccgg tcttgccggc gacgcactcg
     2821 gagatgtagc gctgcaccgg cgcttcccag aagcgctggt tggacatgtt gatggagaac
     2881 tctttcgtgt ccgccggcat ggtgaggttg cgctgcgtga gcacgaagcc gccgatctcg
     2941 cggtcgagcg tgaacacgtg cacgccatgg ccgaaggtga gcaccagcgt ggtggtcggg
     3001 ccgtagacgc agtagccggc ggccacctgc tcgcggccgc gttgcaggta gcactgctcg
     3061 tcgccgccgt cgccgccttc agggttgcgc agcaccgaga agatggtgcc cacggaaatg
     3121 ttcacatcga tgttggacga gccgtcgagc ggatcgaaca gcagcaggta gccgcccttg
     3181 ggatagtcgt cgggaatttt ctgaacagtg tccatttcct ccgaggccag cccggcgaga
     3241 tggccgcccc aggagttggc ctcggtgagg atttcgttcg acagcacgtc gagcttcttc
     3301 tgcgcctcgc cctggatgtt gccggtgccg gcgtcgccga gcacgccgcc cagcgcgccc
     3361 ttgcccagcg caatggagat gcgcttgcag gcgcgggcca ccacttcgat cagcaggcgc
     3421 aggtcgccgc cgatgtagtg gcgcgtgcgc tcttcttcga tcaggaaccg cgtcaacgaa
     3481 atcgagttca tgtgcaggtc tcgaaaagtc ggaaaaatgg gctcaccgca ggttgcctca
     3541 cggtgttata aaacattatc tgatgtatta aatatataat aataaataac atcgccaggg
     3601 gcagtgcggt ggagctcaat tgagcatgcc gggcaccgcc aggcggaacg gatcgatgcg
     3661 cgcgtcgaac ttctcgccgt cgtccgccac catgttgaac tcgccgtgca tggtgcccac
     3721 cggcgtggtc agcggacagc cgctggtgta ctcgaactcg tcgcccggct tgagcaccgg
     3781 ctgctcgccg accacgccgg ggccgcgcac ttcctcggtc ttgccctcgc cgttggtgat
     3841 gacccagtgg cgcgacagca gctgcaccgt gtccggcgaa ttgttctgga tgacgatgtg
     3901 gtaggcgaag agataacggc ccacgtgcgc atccgactgt tccgcgatgt agcgcggcgt
     3961 gaccatgatc ctgatgccgc gcgtcgtagt atcgctcatg agatctccgc aaaactgctg
     4021 cgctcggcac tttgcgcgcg ggcggtgctc ggaatgctca catgctggcg tgcatgctcc
     4081 gcttcccgcg ctgcgtcctc gcgcaaaccg ccttcgctcg cgacgttttt cgaaggtctc
     4141 atggatcaga acattccggt tgccaggcgc gccgcttcgc tcatgcgctc ctgcgaccac
     4201 ggcggctcga acaccagctg cacgtcggtc tcgctcacgc tgggcagctg cgacaccttg
     4261 cccttgatgt cgttgaccag gatgtcgccc atgccgcagc cgggcgcggt cagcgtcatg
     4321 cgcacggtga cgcggcgctt gccgggctcc agcgtttcca ccttgcactc gtagatcagc
     4381 ccgagctcga caatgtcgac cgggatctcc gggtcgtagc aggtcttcag ctgcgcccac
     4441 acggccttct cgacatccgc gtcgctcgca tcgtcggcga cggtgggccc cgcctgcacg
     4501 gtcttgccga tggcgtcggc gtccttgccg tcgatgcgga acaggtggcc ctccacctgc
     4561 acggtgaagc tgccgcccag ggcctgggtg atgacgccct tggcgccctc gggcagttcc
     4621 accttggtgc cggccgggat cagcacgccg atgacatcgc gcgcgaaggt gacgggttcg
     4681 tggagagaca tgaaggggac tgcgctgcag catcgaaaag cgggaaccga ttctaaacgc
     4741 ccgctgtcgg agccgcatct caccaggaag cacccgcggg gggccgcaat gcgcctgacc
     4801 agtttttcgc tcgaaaaccc ggcagcggtg gcggctgccg cgctgatcgt gctgttgttc
     4861 ggcggcatcg cgctgaccaa actgccgatc cagctgctgc ccgacacgcg ccagccgcag
     4921 ctgtttgtct ccgccaactg gcgcgaggcg gcgcccaacg agatcgagga agcgctgatc
     4981 aacccgatcg aggaggcact caagggcatg cccgggctgg tggagatgcg ctccagtgcc
     5041 gcgcgcgggc agagcggggt caatctcaag ttcgaggtgg gcaccgacat gacccgcgtc
     5101 atgctcgacg tggtcaaccg cctgaacacg ctgccgccgc tgccgcccga cgccgaccgg
     5161 ccgcaggtgt ttggcggcga caacaacttc accggcaacg tcgccgcctc gatgctggtg
     5221 cggccgctgc ccggcaacca cacgccggac ctggcggtgc gctaccagaa gctgatggag
     5281 gaagtggtgc agcctcggct ggcgcgcatt cccggcgtgt cgcgggtgaa cctggaaggc
     5341 gggcggccgc gcgaggtgcg ggtggagttc gatccgcaca agctcgccgc cgccggcctg
     5401 acgccgcagc agctggccgc ggccgtggcc ggcgcgcgcg acgcctccgc cggcttcgtc
     5461 gacgtcggcc gccggcagtt caccgtgcgc ttcgccgggc gcgagccggt ggaggagctg
     5521 ggcaacctgg tggtgggctg gagcaacgag cggccgctgt acctgcgcga cgtggccgac
     5581 gtgcacatcg cctggcagga cgcgcaggcc atcacctacc gcaacggcgt gccgggctac
     5641 tacatcacgc tcaaccgcac cagcagttcc aacacggtgg agctgctcga cggcgccaag
     5701 gccgccatcg ccgagctcaa cgaaggtccg ctggcgaagg aaaacctggt gatggacctg
     5761 tcgtgggacg ccagcgtgta cgtgcgccgc gccatcagct tcgtgcagga atcgctgatc
     5821 atcggcgtgc tgctcgccgt caccggcctg tggtatttcc tgcgcgggcc gcgttcgctg
     5881 gtggtgatcg cggcgtcgat tcccatctcg ctgttctttg ccgtcatcac gctgcacgcg
     5941 ctggggcgca cgctcaacgt catctcgctg gcgggcctcg cgttctccac cggcatcgtc
     6001 accgacgccg cgttgatcgt gcagggcaac atcatcaggt atgtgcagga aggacgtagc
     6061 gcgcgcgaag ccacgctgca cggcgcgctg gaagtgttgc cggcgctgct cgcctcgatg
     6121 ctgaccagcg tggccatctt cctgccgatc ctgttcgtgc aggggcttga gggccagctg
     6181 ttccgcgacc tggccatcac catgtcggta tcgcacgcgg cttcgctgct ggtcgccatg
     6241 acggtactgc cggccgccag ccgctgggcg ctggcgcaga aacttccctc ggatgcgcac
     6301 cagcactggt ggaaggcgat gacgcggctg tccatgcgcc tgaccgaccg gccgcaggcg
     6361 cgctgggcct ggatcgccac gctggtggcc ggcgcggttc tggccatcga cgtgatggcg
     6421 ccgcaggtgg attacctgcc ggtggcgcag accgacaaca tcttctccaa cttccgcctg
     6481 ccaccgggcg gaaatctcat tacgctgcag aaagaattcg cgccggtggt gatcgcgcgc
     6541 atgcagccgt acctcgaagg caaaaaggaa ccggcggtga agtactacaa cctgtcggcc
     6601 ttcggtggcc agttcggcct gatggtggcg tacgcgaaga acccgcgcga catccagaag
     6661 ctggcgaaga cgctgcgcga ggaaattctc gtgggtctgc ccgatgtgag tgtttacgtg
     6721 cagcgcggct cgctgctcga cgtcgacggc ggcaattcgc gcgacatcga gctcgacttc
     6781 cagggcccgg acctcgacgg cctgatgaag gccgcgggcg cggcgatgaa ggccattccg
     6841 caggcgattc ccggcacgta cccgcaggca tttccgtcgc tcgaactgaa ccagccggag
     6901 atcaccctgg ccccggacga gtggcgcatc g
//
LOCUS       DASZZC010000637         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1019081,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000637 DASZZC010000000
VERSION     DASZZC010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019081"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..976
                     /gene="cysW"
                     /locus_tag="VHE37_13575"
     CDS             71..976
                     /gene="cysW"
                     /locus_tag="VHE37_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HVT36613.1"
                     /translation="MITPADHTLLDPPRLPFEPLIQKNAAITEPAWVRRLLTVLALLF
                     LSAFLLLPLLTVFTEALRNGASAYLDALVEPDALAAVKLTLIAAGIAVPLNLVFGLAA
                     AWAITKFEFRGKAFLITLIDLPFSVSPVVSGLIYVLMFGAQGYLGPWLDEHGVRIIFA
                     VPGIVLATIFVTFPFIARELIPLMQEQGTDAEQAALSLGASGWQTFWRVTLPNIRWGL
                     LYGVLLCNARAMGEFGAVSVVSGHIRGQTDTMPLHIEILYNEYSFAAAFAVASLLAGL
                     ALVTLGVKSFLEWRYGDELAATRRH"
     gene            984..>1503
                     /locus_tag="VHE37_13580"
     CDS             984..>1503
                     /locus_tag="VHE37_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004731677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT36614.1"
                     /translation="MDIQIRGIQKSFGSFQALSGVDLDIASGELIALLGPSGSGKTTL
                     LRAIAGLEFADSGQVLFGGQDMARVPVRERRVGFVFQHYALFKHMTVADNIAFGLRVR
                     GKRERPPENEIARRVRELLELVQLAGLERRYPAQLSGGQRQRVALGRALAIDPEMLLL
                     DEPLSNLDARTRL"
BASE COUNT          207 a          513 c          504 g          279 t
ORIGIN      
        1 tgatctcgtt ctgcatgctg ctggcgatca acctgctgca ggcctggtcc cgcaaccggg
       61 gtggccgcaa atgatcacgc cggccgatca caccctgctt gatccgccca ggctgccgtt
      121 cgagccgctg atccagaaaa atgctgcgat caccgaaccc gcgtgggtcc gccggctgct
      181 gacggtgctg gcgctgctgt tcctgtcggc gttcctgctg ctgccgctgc tgacggtttt
      241 caccgaggcg ctgcgcaacg gcgcgtcggc ttacctagac gcactggtcg aacccgacgc
      301 gctggccgcg gtgaagctca cgctgatcgc cgccggtatc gcggtgccgc tgaacctggt
      361 gttcggcctt gccgcggcct gggccatcac caagttcgag ttccgcggca aggccttcct
      421 gatcacgctg atcgacctgc cgttctcggt ctcgccggtg gtctcgggcc tgatctacgt
      481 gctgatgttc ggcgcgcagg gttatctcgg cccgtggctg gacgagcacg gcgtcaggat
      541 catcttcgcc gtgccgggca tcgtgctggc gacgatcttc gtcaccttcc cgttcatcgc
      601 acgcgaactg atcccgctga tgcaggaaca gggcacggat gccgagcagg ccgcgctgtc
      661 gctgggcgcc tcgggctggc agaccttctg gcgcgtgacg ctgccgaaca tccgctgggg
      721 cctgctctac ggcgtgctgc tgtgcaacgc gcgcgccatg ggcgagttcg gcgcggtgtc
      781 ggtggtgtcg ggccatatcc gcgggcagac cgacaccatg ccgctgcaca tcgaaatcct
      841 ctacaacgaa tattccttcg ccgctgcctt cgcggttgcc tcgctgctgg ccgggctcgc
      901 gctggtcacg ctcggcgtga agagcttcct cgaatggcgc tacggcgacg aactcgccgc
      961 cacgcgccgg cattgagatc cacatggaca tccagatccg cggcatccag aaatccttcg
     1021 gcagtttcca ggcgctgtcc ggggtcgacc tcgacattgc ctccggcgag ctgatcgcat
     1081 tgctcggccc gtcgggctcg ggcaagacca cgctgttgcg cgccatcgcc gggctggagt
     1141 tcgccgacag cggccaggtg ctgttcggcg gccaggacat ggcgcgcgtg ccggtacgcg
     1201 agcgccgcgt gggcttcgtg ttccagcact acgccctgtt caagcacatg acggtggcgg
     1261 acaacatcgc cttcggcctg cgcgtgcgcg gcaagcgcga gcgcccgcca gagaatgaga
     1321 tcgcgcgccg cgtgcgcgaa ctgctggagc tggtgcagct ggccgggctg gagcgccgct
     1381 accccgcgca gctgtccggc gggcagcgcc agcgcgtggc gctggggcgc gcgctggcga
     1441 tcgatccgga aatgctgctg ctggacgagc cgctgtcgaa tctcgatgcg cgcacccggc
     1501 ttt
//
LOCUS       DASZZC010000638         4928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1147406,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000638 DASZZC010000000
VERSION     DASZZC010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147406"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VHE37_13585"
     CDS             complement(<1..597)
                     /locus_tag="VHE37_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HVT36615.1"
                     /translation="MKKPAAFAVVAMLLFPLSGWSQALPDSDGDGVPDANDVCPHTPP
                     HAIVDSQGCATDEDFDGVPDGLDQCPHTEFGSAVDANGCSANQRPGAAASMPSPAPAA
                     ESVAPPAQPSMPAAAPKRSPAAVAAPLPEASPTAPESRAPISPLAPDAAALMNGPAPT
                     KPAAVAPPKAQVVPAPAPEPVAAPAAVAAPAPETSAPEL"
     gene            complement(729..1919)
                     /locus_tag="VHE37_13590"
     CDS             complement(729..1919)
                     /locus_tag="VHE37_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT36616.1"
                     /translation="MNKLVRAGSCLALAFAAFSASAADKEMRPYLDFGYSHVFSDSDR
                     KTNDGNGAYVGVGKAINDFWGWEFSGYYNNYSGDSSNPVSGREYGGKLDGMFFYSRKP
                     SFSPYLAVGVGGLRTDKKNAPSASSTDPTVDAGLGFTSFFPVTSTFDLGLRADLRYRW
                     LDLKDIPGAGSVSEPVLRVGLVIPMGTGAVAGAVAANAASALGPHISTGHITANDQDG
                     DGVLDDVDQCPNTPAGWLVDARGCPIDSDHDGVPDNLDKCPGTPPGISVDATGCPISV
                     AAAGANRSFENTNFAFDKSDLTDYAKAILDNAANVINGLVQKYPSLKVDVSGHTDYIG
                     TEAYNMGLSERRANAVKGYLTRKGVDANRINTFSYGETKPIAPNETSEGRALNRRAEI
                     RTHE"
     gene            2105..3139
                     /gene="sohB"
                     /locus_tag="VHE37_13595"
     CDS             2105..3139
                     /gene="sohB"
                     /locus_tag="VHE37_13595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HVT36617.1"
                     /translation="MLEFFSQYGLFLAKTVTWIAAILATAAGLVSIMREARQHHTGDH
                     LQIRNLNQRYEQMADILNAELLSGHELKELGKKRKAERKAREKAEKHGHPPRPRVFTL
                     DFVGDLNASAVDALREEISAVLQVARKEDEVLVRLQSEGGLVHSYGLAASQLQRIRQQ
                     GYRLTAAVDKVAASGGYLMACVADRILAAPFAIVGSIGVVAQLPNFNRLLKKHEIDFE
                     LHTAGEFKRTLTLFGENTEEGRAKFRQEIEDTHTLFKSFVAENRRNLDLEKVATGEHW
                     FGARALSLKLVDELKTSDDYLLERINQADVYEVIFKRRQSLADRMAHGLARLGSGLRE
                     AVSRAGSPPA"
     gene            3212..4657
                     /locus_tag="VHE37_13600"
     CDS             3212..4657
                     /locus_tag="VHE37_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HVT36618.1"
                     /translation="MSRATAIRIFAPLAVSASLLAAAFMMSSAPSSVSAGSAVVGATA
                     TNASLPLTAGVPSLAPMLKEVMPSVVNIAVTSKQQIRNPLMDDPFFRRFFGAPDDEEQ
                     QQPEEQEAQAIGSGVIVDAEKGYVITNNHVVDGADTIKVRLSDDREFDAKLIGKDPQT
                     DVAVLQIKADHLHAIPVGDSSRLEVGDFVVAIGSPFNLRQTVTSGIVSGLSRRTGISE
                     GGYEDFIQTDASINPGNSGGALVNLRGELIGIPSNILSRSGGNIGIGFAIPVNLAKGV
                     MTQLIATGAVERGRIGVFGQDLTPELAKAFGLTSTHGAVVTKVVPKSPADKAGIKTED
                     VILAINGHDVEDFAQLRNTVGLMRIGETVDLKIQRDGKTRNVSVTISKDVETVKAGGL
                     LHPKLKGATFAPVDERNAQDTDGLGVIVQKIEPRSPAAHSGLKEGDIIVGVNRHPVSS
                     YEEFTKLVDPKQAQLLLNIRRGGGALFILLQ"
     gene            4729..>4928
                     /locus_tag="VHE37_13605"
     CDS             4729..>4928
                     /locus_tag="VHE37_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36619.1"
                     /translation="MDSEIQQLEERVQRLMTAYRQSRLEHKRIVQERDRLLALNAELR
                     RRIEGIVDRIRALESEPERDRE"
BASE COUNT          926 a         1537 c         1603 g          862 t
ORIGIN      
        1 caactcgggc gccgaggtct ctggcgccgg cgcggcgacc gcagcgggcg ctgctaccgg
       61 ctcgggcgcc ggagcaggta ctacctgcgc tttgggcgga gctacagcgg ccggcttggt
      121 cggcgcaggt ccattcatca atgcagcggc atccggcgcg agcgggctga tgggagcgcg
      181 gctctccggc gcagttggac tcgcctccgg aagaggcgcg gctacggcgg cgggtgaccg
      241 cttgggcgcc gccgctggca tggatggttg tgcaggcgga gcaacggact ctgcggcagg
      301 agcaggggac ggcatggaag ccgccgcacc cggacgctgg tttgccgagc atccgttggc
      361 atccacggcg ctgccgaatt cggtgtgcgg gcactggtcc aggccgtccg gtacgccatc
      421 gaaatcttcg tcggttgcac agccctggct gtcgacgatg gcgtgaggtg gggtatgcgg
      481 acagacgtcg ttggcgtcag gcacaccgtc gccatcactg tcgggcagcg cctggctcca
      541 gccggacaaa gggaacagca gcattgctac taccgcgaag gccgcaggct ttttcatgat
      601 ctggagattc cctttttcga gaaaccgctg aagcgccggt agcgtaaacg gcggctgcca
      661 tcgagttcaa gcagacgtcg gccaaaaaaa agcccccgca gtgcgggggc tttggtaaag
      721 cagacagctt attcgtgcgt gcggatttcc gcgcgacggt tcagcgcacg accttccgac
      781 gtctcgttcg gcgcgatcgg cttggtctcg ccgtacgaga aggtgttgat gcggttggca
      841 tccacgccct tacgggtgag gtagcccttg accgcattgg cacggcgttc cgacaggccc
      901 atgttgtagg cctcggtgcc gatgtagtcg gtatgacccg acacgtccac cttcaggctc
      961 ggatacttct gtaccaggcc gttgattacg ttcgctgcgt tgtcgaggat cgccttggcg
     1021 tagtcggtca ggtcagactt gtcgaaggcg aagttcgtgt tctcgaagct gcggttggcg
     1081 cctgcagcgg ccacactgat cgggcagccg gtcgcgtcga cgctgatgcc cggaggcgtg
     1141 ccagggcact tgtcgaggtt gtcgggaaca ccgtcgtggt cgctgtcgat cgggcagcca
     1201 cgcgcatcca ccagccagcc ggccggggtg ttcgggcact ggtcgacgtc atccagcacg
     1261 ccgtcgccgt cctgatcgtt ggccgtgatg tggccggtgg agatgtgcgg gccgagggcc
     1321 gacgccgcgt tggcggcaac cgcgcccgcc acagcgccgg tgcccatcgg gatcaccagg
     1381 ccgacgcgca gcacgggttc gcttaccgag ccggcgccag ggatgtcctt gagatccagc
     1441 cagcggtagc gcagatcggc gcgcagtccg aggtcgaagg tgctggtcac cgggaagaat
     1501 gaggtgaaac cgaggcctgc gtccacagtg ggatcggtcg agctggccga cggggcattc
     1561 ttcttgtccg tgcgcagtcc gccgacgccg accgcgaggt agggcgagaa ggacggcttg
     1621 cgcgaataga agaacatgcc atcgagcttg ccgccatact cgcggcccga aaccgggtta
     1681 ctcgagtcgc ccgagtagtt gttgtagtag ccggagaact cccagcccca gaagtcgttg
     1741 atggccttgc cgacgccaac ataggcgccg ttgccgtcgt tggtcttgcg atcgctgtcg
     1801 ctgaagacgt ggctgtagcc gaagtccaag taaggacgca tctccttgtc ggctgcggag
     1861 gcggaaaaag ccgcgaaggc cagagcgagg cagctacctg cccgaacgag tttattcatc
     1921 ttcgaatctc caaccactcc actttgcgtt tatagaatta gggatgcctg aaagtgaatt
     1981 aaaccactaa catccgcctt aacacaaatt aattgttgat taccccggcg gctttcgccc
     2041 tcatgcttga aaagcgggcg catgtccgca tacatgccga ctattccctg atccagatct
     2101 gtccatgctc gaattcttca gccagtacgg cctgttcctc gccaagacgg ttacctggat
     2161 cgctgccatc ctggcgacag ccgcggggct ggtctcgatc atgcgagaag cgcgccaaca
     2221 tcataccggc gatcacctgc agatccgcaa tctgaaccag cgctacgaac agatggcgga
     2281 catcctcaat gccgagttgc tgtcggggca tgaattgaag gaactcggca aaaagcgcaa
     2341 ggccgagcgc aaggcccgcg aaaaagccga aaagcatggg cacccgccgc gtccgcgggt
     2401 gttcaccctg gattttgtcg gtgatctcaa tgccagcgcc gtcgacgcgc tgcgcgaaga
     2461 gatcagcgcg gtgctgcagg tggcgcgcaa ggaagacgag gtgctggtac gcctgcagag
     2521 cgaaggaggc ctggtgcatt cctatggtct ggccgcgtcg caactgcagc gcatccgtca
     2581 gcagggttac cgcctgaccg cggccgtgga caaggtggcc gccagcggcg gttatctgat
     2641 ggcctgcgtt gcggaccgca tcctggctgc gccgttcgcc atcgtcggct ccatcggcgt
     2701 ggttgcgcag ctacccaact tcaaccggct gctgaagaag cacgagatcg attttgaatt
     2761 gcataccgcc ggcgaattca agcgcacgct gacactgttt ggcgagaata cggaagaggg
     2821 ccgcgccaag ttccgccagg aaatcgaaga cacgcacaca ctgttcaagt cgttcgtcgc
     2881 cgagaaccgg cgcaacctgg atctggagaa ggtggccacc ggcgagcact ggttcggcgc
     2941 gcgggcgctt tcgctgaaac tggtggacga attgaagacc agcgacgatt acctgctgga
     3001 gcgcatcaac caggccgatg tttacgaggt tatattcaag cgcaggcaat cgctggcgga
     3061 ccgcatggcg cacggcctgg cccgtctggg cagcggcctg cgcgaagcag tgtcgcgtgc
     3121 cggctcgccc ccggcgtgaa acttggacgc ggcagcgcgg tcatagcgcg gctgcaggct
     3181 cgactattta gccagaggaa attttcaaac catgtcacgt gcaacagcca tccgtatctt
     3241 tgccccgctc gccgtttcgg cttccctgct ggcagcggcg ttcatgatgt cgtcggcacc
     3301 gtcgtcggtc tccgccggct cggcggtggt cggtgccact gccaccaacg cttcgctgcc
     3361 tctcaccgcc ggcgtgcctt cactggcgcc gatgctgaag gaagtgatgc cctcggtggt
     3421 caatatcgcg gtcaccagca agcagcagat ccgcaatccg ctgatggacg acccgttctt
     3481 ccgccgcttc ttcggcgcgc cggatgacga agaacagcag cagccggaag agcaggaggc
     3541 gcaggccatc ggctcgggcg tgatcgtcga tgccgagaag ggttacgtca tcaccaacaa
     3601 ccacgtggtc gacggcgccg acaccatcaa ggtgcgcctg tccgatgacc gcgaattcga
     3661 cgccaagctg atcggcaagg atcctcagac cgacgtcgcc gtgctgcaga tcaaggccga
     3721 tcacctgcac gccatcccgg tcggtgattc gtcgcggctg gaagtgggcg atttcgttgt
     3781 cgccatcggc tcgccgttca acctgcgcca gaccgtgacc agcggcatcg tctcgggcct
     3841 gtcacgccgc accggcatca gcgaaggcgg ctacgaggac ttcatccaga ccgacgcctc
     3901 gatcaacccc ggcaattccg gcggcgcgct ggtcaacctg cgtggcgaac tgatcggcat
     3961 cccgagcaac atcctgtcgc gttccggcgg caacatcggc atcggcttcg ccatcccggt
     4021 caacctggcc aagggcgtga tgacgcagct gatcgccacc ggcgcggtcg agcgcggccg
     4081 catcggcgtg ttcggccagg acctgacgcc ggaactggcc aaggccttcg gcctgaccag
     4141 cacgcacggc gcagtggtca ccaaggtggt gccgaagtcg ccggccgaca aggcgggcat
     4201 caagaccgaa gacgtgatcc tggccatcaa tggtcacgat gtggaggact tcgcccagct
     4261 gcgcaacacc gtgggcctga tgcgcatcgg cgaaaccgtc gatctcaaga tccagcgcga
     4321 cggcaagacg cgcaacgtct cggtcaccat cagcaaggat gtggagacgg tgaaagccgg
     4381 cggcctgctg catcccaagc tcaagggcgc caccttcgcg ccggtggacg agcgcaacgc
     4441 gcaggatacc gacggcctgg gcgtgatcgt gcagaagatc gagccgcggt cgccggcggc
     4501 gcacagcggg ctgaaggaag gcgacatcat cgtcggcgtc aaccgccatc cggtgagcag
     4561 ctatgaggaa ttcaccaagc tggtggaccc caagcaggcg cagctgctgc tcaacatccg
     4621 ccgcggcggt ggagcgctgt tcatcctgct ccagtaaata aaccctctgc tcaactttgc
     4681 ctggcgccgg cctatactag gccggcgctt tttttggggc agggacggat ggattccgag
     4741 atacagcagc ttgaggaacg ggtgcagcgg ctgatgaccg cctatcgaca gtcccgcctc
     4801 gagcacaagc gcatcgtgca ggaacgcgac cgcctgctgg cgctcaacgc ggaactgcgc
     4861 cgacgcatcg aaggcatcgt cgaccgcatc cgcgcgctgg aatccgagcc cgagcgcgat
     4921 cgtgaaca
//
LOCUS       DASZZC010000639         7031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_500759,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000639 DASZZC010000000
VERSION     DASZZC010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500759"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VHE37_13610"
     CDS             <1..341
                     /locus_tag="VHE37_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT36620.1"
                     /translation="AALARGAGLYKKNCSDCHGEDGRGQPPDEPPLAGNAMLTAPDPG
                     NAILMVLQGGYAPGTRGNPRPYGMPPYAQALSEEDIAALLSYARQAWGNAAAPVDAQQ
                     VARVRGIPQE"
     gene            345..572
                     /locus_tag="VHE37_13615"
     CDS             345..572
                     /locus_tag="VHE37_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HVT36621.1"
                     /translation="MSEPEPPPPDRAPTLLQVIGSVLAAMFGVQSSKARQRDFTRGRP
                     LHYIVVGILAVLVLIAVLLGLVRLLLHNAGM"
     gene            907..1101
                     /locus_tag="VHE37_13620"
     CDS             907..1101
                     /locus_tag="VHE37_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36622.1"
                     /translation="MFWIKWVVAGEAGLLVEQPVMQAAAALRAHQPKAAPAAQERTGG
                     EPPVAATEHMRAVQYWSEIH"
     gene            1139..2314
                     /locus_tag="VHE37_13625"
     CDS             1139..2314
                     /locus_tag="VHE37_13625"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVT36623.1"
                     /translation="MPVQKVLSSGRVPVKIYTQDIAANAQAQLANIATLPFVHHHVAA
                     MPDVHLGIGAAVGAVIPTRGAIIPAAVGVDIGCGMNAVRVSLKGRDLPDDLKKIRAQI
                     ERDVPVGFGAHQEARWQTAALTRVAPGLLRVQEKWPQLQADWRGQMGSLGGGNHFIEL
                     CLDENDNVWVMLHSGSRGIGNRIGQFFIEAARREMERHFIQLPDRDLAYLAEGSELFE
                     DYIEAVGWAQDYARENRAAMMQLVLQALARFLPPFTLTQEAISCHHNYVAREAHYGAE
                     VWVTRKGAIRAGAGELGIIPGSMGAHSYIVRGKGSAESLCSCAHGAGRRMSRNEAQKH
                     FTVDDLRRQTTGVECRKDGGVLDEIPGAYKDIDTVMANQSDLVEIVHTLKQVLCVKG"
     gene            complement(2445..7013)
                     /locus_tag="VHE37_13630"
     CDS             complement(2445..7013)
                     /locus_tag="VHE37_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36624.1"
                     /translation="MTRLFTFSDFDGDGSAAALVRDDNGSFYGISNFFGQVGSGTVYR
                     LDPVSDPAPAAPSISLTADHYQVTAGSNVDLSWDVSGASNCVADGYVWSEQVPFNGEH
                     LVAVSQVGSNTFSLICSAVGATAIQSVVVTGLPPAQVSLNASPASVRVGQNTTLTWNS
                     SNASSCLASGDWSGVRALSGSAQVLVGHAGTNTFTLACDGASKSVSVNGIPPPGISLT
                     PNPDTVAVGGTSTLTWNVSNATSCSASGAWSGAKALSGSQSVTAAQSGTYTYTLTCSG
                     AGGSASQSATVTALQPPGVGLFNDPNTPYVAVGANITLNWNSARAQSCTASGAWSGPR
                     ALSGSETVVLNSPGDATFALTCSGIGGTSSHSMTLTAFLPPVISLNASPSTLGAGQSA
                     TLNWNATDAQLCSASGGWSGSKSMNGSEGVTAATPGTVSYTLTCQGWGGTSAQSTAVT
                     YLANPTVALAANPTAVTVGQSTMLNWNAENASSCVASGVWAGSLPISGSQIVVVQNAG
                     ANSYTLTCTGSGGSATQTTSVNGIPLMPTLSFAANPVAVPVGQTSTLRWSSTDAQSCT
                     ASGAWSGAQSLSGSAVVTISAAGPATYSLSCTGPGGTTSNAVTISGLPPPTLTVSANA
                     TALILGQSSTISWSSTNTDSCNASGAWSGAQPTSGSASVTPSVAGASNYTLACTGSGG
                     SVTQSVTINATPPAILHFAAEPAAVPVGQSSTLTWSSTDTTSCTASGAWNGTQATSGT
                     ATMTITTAGPTSYSLSCSGAGGTASSSVTVTGLPPPTLSLAANPAALILGQNTMLSWT
                     TTNADSCTASGAWSGTQAASGSASVTPMSTGANSYMLTCTGSGGSIAQTAVVNATPPA
                     TLSFSANPAAVAVGQASTLTWSSTGTTSCTASGGWSGTQATTGSADVTIAAAGAANYA
                     LSCSGAGGTAVNTVTVMGVPPPAINLAATPPSGVVGQSAHLTWSSTDAQSCTAFGNWS
                     GARPVSGSADAPIYNVGSNNEYQLRCTGVGGTSTASVLVTGRPAPSVSLIGSYSAHAG
                     DGTTLRWDSVNVQTCTASGGWSGTLSTSGTQAITVAEGATSYGLACTGSDGSVSTSVT
                     ITGYPRPTLNLAASASTLYVGDSVTLTWSSDKLNSCVASGAWSGSKANSGSESVAVAA
                     AGSNEFWLTCQDALGPWSAEKTVTATVPTTLNFNSSSSEVPVGQSVSLNWTTGNADSC
                     SASGAWSGAKAVSGSQSVLISNPGNNNFGLSCTGSGGNPIVLVTVKGMPVVSLSASPT
                     SVKVGGTSKLTWSSTYSNSCTASGAWSGSQSTSGTKTVTVSSAGNKVYTLACSGLGGN
                     TTVSTTVTGVPAPTVTITANPASVIVGQNTTLTWSSTDATSCTASGSWSGTKSTSGSA
                     SVAITSTGSKTFTLACTGVGGSASKSATATGVNPTVNFATTSEATAPGATSTTNVLVT
                     LSGATSNTVTVPFTTGGTAGTTTYNVAPSTKKLTIAAGATSGYITVNVTSAVCNKTVT
                     LTLGTPTNGTLGTAKTNTLTIKSATGCP"
BASE COUNT         1243 a         2207 c         2310 g         1271 t
ORIGIN      
        1 cggcggcgct cgcgcgcggc gccgggctat acaagaagaa ttgttccgac tgccatggcg
       61 aggacggacg tggccagccg ccggacgaac ccccgctggc cggcaacgcc atgctcaccg
      121 cgcccgatcc gggcaatgcg atcctcatgg tgctgcaggg cggctatgcg ccgggcacgc
      181 gcggcaaccc gcgtccgtac ggcatgccgc cgtacgctca ggcgctgagt gaagaagaca
      241 tcgccgcctt gctcagctac gcgcgccagg cctggggcaa cgccgccgcg ccggtcgatg
      301 cgcagcaggt ggcacgtgtg cgcggcatcc cgcaggagtg aaccatgagt gaacccgagc
      361 cgccgccgcc cgatcgcgcg cccaccctgc tgcaggtgat cggcagcgtg ctcgccgcca
      421 tgttcggcgt gcaaagctcg aaggcgcgcc agcgtgattt cacccggggc aggccgttgc
      481 actacatcgt ggtcggcatc ctggcggtgc tggtgctgat cgccgtcctg ctcggcctgg
      541 tgcggctgct gctgcataac gcgggaatgt aatgcgcttg cgacgcccag ggcgcaggcg
      601 tacagtgcgc gcccttcgca cctcgcgtgc gcatttctgc agagactttt tgatgaaccc
      661 ggtgttcgcc attccgcgta gtcagatgca gcggctcaat tgccgcggcc tgaccctgcg
      721 cgcgcgcgca ctgcgttccg ccagtgagcg ccgcgccctt cgcacctgat ctgatccaca
      781 catttcggag gtcggtgcga accgacctcc cgagttgcaa gaacgccccg ggaggcttga
      841 acccccgggg cgtttttgtt tttggcgttg cacggggtac ccaggcagca caacaggaga
      901 atgcacatgt tctggatcaa gtgggtggtg gccggcgaag ccggcttgct ggtcgagcag
      961 ccggtgatgc aggcggcagc ggcgctgcgc gcgcaccagc ccaaggccgc gccggcagcg
     1021 caggaacgca ccggcggcga gccgccggtg gccgcgactg agcacatgcg cgcggtgcaa
     1081 tactggtccg agatccactg agcgctagcg aatttccaac aaccgagtct gaagtgtcat
     1141 gcccgtacag aaagtgctga gcagcggccg cgtgccggtg aagatctaca cgcaggacat
     1201 cgccgccaac gcgcaggcgc agctggccaa catcgccacg ctgccgttcg tgcaccacca
     1261 cgtggcggcg atgcccgacg tgcacctggg catcggcgcg gcggtgggcg cggtgattcc
     1321 cacgcgcggc gccatcatcc cggccgcggt cggcgtcgac atcggctgcg gcatgaacgc
     1381 ggtgcgcgtg tcgctgaaag gccgcgacct gcccgatgac ctgaagaaga tccgcgcaca
     1441 gatcgagcgc gatgtgccgg tgggtttcgg cgcgcaccag gaggctcgct ggcagacggc
     1501 ggcgctgacg cgcgtcgcgc ccggcctgct gcgcgtgcag gaaaaatggc cacagctgca
     1561 ggccgactgg cgcgggcaga tgggctcgct cggcggcggc aatcacttca tcgagctgtg
     1621 cctcgacgaa aacgacaatg tttgggtgat gctgcactcg ggctcgcgcg gcatcggcaa
     1681 ccgcatcggc cagttcttca tcgaggccgc ccgcagggag atggagcgcc atttcatcca
     1741 gctgccggac cgtgatctcg cttacctggc tgagggcagt gaactgttcg aggactacat
     1801 cgaggcggtg ggctgggcgc aggactacgc gcgcgagaac cgcgccgcga tgatgcagct
     1861 ggtgctgcag gcgctggcgc gcttcctgcc gccgttcacg ctgacgcagg aagcaatcag
     1921 ctgccatcac aactacgtgg cgcgcgaagc gcactacggc gccgaggtgt gggtgacgcg
     1981 caagggcgcg atccgcgccg gcgcgggcga actgggtatc attcccggca gcatgggcgc
     2041 acactcttac atcgtgcgcg gcaagggcag cgcggaatcg ctgtgctcat gcgcgcacgg
     2101 cgccggccgt cgcatgtcgc gcaacgaggc gcagaagcat ttcaccgtcg acgatctcag
     2161 gcgccagacc accggcgtgg aatgccgcaa ggacggcggc gtgctcgacg agatccccgg
     2221 cgcctacaag gacatcgaca cggtgatggc caaccagagc gacctggtgg agatcgtgca
     2281 caccctcaag caggtgctgt gcgtgaaggg ctgagcgcgc aaacaggtcc accgcacctg
     2341 cattttgagt gttattaaat atagtctgtt ttaataatca gacaattttt tataacacac
     2401 cggaaggcag tgcggtgggg ctgtttgttg cggcctctaa ttttttacgg acaacctgtc
     2461 gccgacttga tggtcagcgt gttggtcttg gccgtgccca gagtgccatt ggtgggtgta
     2521 cccagcgtca gcgtcaccgt cttgttgcac acggcgctgg tgacattgac cgtgatgtaa
     2581 ccgctggtgg cgcccgccgc aatggtcagc ttcttggtac tcggggccac gttgtaagtg
     2641 gtggtgcccg cggtgccgcc ggtggtgaac ggcaccgtca cagtgttgga agtggcgccc
     2701 gacagcgtca ccagaacatt ggtcgtcgac gtcgcccccg gcgctgtcgc ttcagaggtg
     2761 gttgcgaaat tcaccgtggg attcacgccg gttgccgtcg cgctcttgct cgccgagccg
     2821 ccgaccccgg tgcaggcgag ggtgaaggtt ttcgagcccg tgctggtgat ggccacgctg
     2881 gcgctgcccg aagtggactt ggtgccgctc cacgagccgg atgcggtgca cgaggtggcg
     2941 tcggtggaac tccaggtaag cgtggtgttc tggccgacga tgactgaagc gggattcgcg
     3001 gtgatcgtca ccgtgggcgc cggcacaccg gtcaccgtag tgctgacggt cgtattgccc
     3061 ccaagccccg agcaggccag cgtatagacc ttattgccgg cgctgctaac cgttacagtc
     3121 ttggtgccgg aggttgattg cgatccgctc caggcgcccg atgccgtgca ggaattcgag
     3181 taggtcgaac tccaggtcag cttcgaagtg ccgcccacct tcactgacgt ggggctcgcc
     3241 gacagcgaga cgacaggcat gcccttgacc gtaaccagta cgatcggatt gccgccactg
     3301 ccagtacagg acagcccgaa gttattgttg cccgggttcg agatgagcac gctttgcgat
     3361 cccgataccg ctttggcgcc actccaagct ccggaggcac tgcaactgtc cgcgttgccg
     3421 gtcgtccaat tcagcgaaac gctttggcct accggaactt cgctactgct ggaattgaaa
     3481 ttaagcgtgg tcggaacggt ggcggtaacc gtcttctcag cggaccaagg accaagcgcg
     3541 tcctggcagg tcagccagaa ttcgttgctg ccggcagcgg ccaccgctac cgattcgctg
     3601 ccactgtttg ccttggaccc actccaagcg cccgaagcga cgcagctgtt cagcttgtcg
     3661 gaactccagg tcagcgttac ggaatcgcct acgtagagcg tcgacgcact agccgcgaga
     3721 tttaacgttg ggcgcgggta accggtgatg gtgaccgacg tcgacacgct gccgtcgcta
     3781 ccggtacagg cgagcccata tgaggttgcg ccttccgcca cagtaattgc ctgcgttccg
     3841 gaagtgctca aggttccgct ccagccgcca ctcgcggtgc aggtctggac gttcactgaa
     3901 tcccagcgca gcgttgtgcc gtcgccagca tgcgccgagt aactgccaat cagtgaaacc
     3961 gacggcgccg gccggcccgt gaccagtacg gaggccgtgc tggtgccgcc cacgcccgtg
     4021 cagcgcagct ggtactcatt gttactgcca acgttataga tcggcgcgtc ggcagagccg
     4081 gacaccggcc gcgcaccgct ccaatttcca aatgcggtgc agctctgggc gtccgtgcta
     4141 gaccatgtga gatgcgcgga ttgaccgact acgccggaag gcggagtcgc tgccagattg
     4201 attgccggcg gagggacacc catcaccgtc accgtgttca cagcagtacc gccggcgccg
     4261 ctacacgaca gcgcataatt cgccgctcct gcagcggcaa tggtcacatc tgcggatccg
     4321 gtagttgctt gggttccgct ccagccgccc gacgcggtac acgacgtggt gccggtggat
     4381 gaccaagtca gcgtgctggc ttggcctacg gctaccgctg cgggattggc ggagaagctc
     4441 agcgtggcgg gcggtgttgc gttcacaact gccgtttgcg caatgctgcc accgctgccg
     4501 gtgcaggtca gcatgtagct gttcgcccct gtactcatcg gcgtgacgct tgcggagcct
     4561 gatgccgctt gggtaccgga ccatgcgccg gaggcggtgc aggaatcggc attggtcgta
     4621 gtccagctca gcatcgtgtt ttgaccaagg atcagcgcgg ccggattcgc agcgagactt
     4681 agcgtcggcg gcgggagtcc tgtgaccgtc actgagctcg acgccgttcc gccggctcca
     4741 ctgcatgaca gcgagtagct cgtcgggcca gccgtcgtga ttgtcatcgt cgccgtaccg
     4801 gacgttgctt gcgttccgtt ccatgcaccc gaagccgtgc acgaagtggt atcggtcgat
     4861 gaccaggtca gcgtactgct ctggccgacc ggcacggctg cgggctcggc cgcgaagtgc
     4921 aggatcgccg gcggtgtcgc gttgattgtc accgactgcg tgacggagcc gccgcttccg
     4981 gtgcaagcga gcgtgtagtt gctggcgccg gcaacggacg gtgtcacgct cgcgctgccc
     5041 gaggtaggtt gtgcaccgga ccacgcgccg gaagcgttgc aggaatccgt gttggtcgag
     5101 ctccagctga tggtggaact ctggcccaga atcagcgccg tcgcattggc tgatacggtg
     5161 agggtaggcg gaggcagccc gctgatggtt accgcgttcg acgtggttcc gccagggccg
     5221 gtgcacgaca acgagtaagt cgcgggaccg gccgcgctga ttgtcaccac ggcagaacca
     5281 gacagggatt gagcgccaga ccacgcacca cttgctgtgc agctttgcgc atcggtggac
     5341 gaccaacgca gcgtgctggt ctggccgacg ggaaccgcaa ccgggttggc ggcgaaactg
     5401 agtgtcggca tcaacgggat gccattcacg ctcgtggttt gcgtcgcgct gcctccgctt
     5461 ccggtgcaag tgagtgtata gctgttggcg ccggcattct gcacaaccac gatctgcgat
     5521 cccgaaatcg gcaggcttcc cgcccacacg ccagacgcaa cacagctgga tgcattttcc
     5581 gcattccagt tcagcatggt gctttggccg accgtgaccg cggtggggtt tgccgccagc
     5641 gcgactgtcg gattggccag gtatgtcaca gccgtgctct gcgcggatgt cccaccccaa
     5701 ccctgacaag tcagcgtata gctgacggtg cccggcgttg ccgccgtcac gccttcggag
     5761 ccgttcattg attttgaacc tgaccacccg ccgctggcgc tgcatagctg agcgtcagtc
     5821 gcattccagt tcagcgtcgc gctctgccct gctccgagtg tcgacggaga agcgttcaac
     5881 gagatgaccg gcggcaggaa ggcggtcagg gtcatgctat gcgaactcgt gccaccgatg
     5941 ccgctgcagg tcagcgcgaa ggtcgcgtcg cccgggctat tcagcaccac cgtttcactg
     6001 cccgaaagag cccgcggtcc ggaccaagca ccacttgcgg tgcagctctg cgcacgcgcc
     6061 gagttccagt tcagcgtgat gttggcgccc acggcgacat agggcgtatt ggggtcgttg
     6121 aagagaccca cgccgggcgg ctgcaacgca gtgaccgtcg cactctgcga tgcgctgccg
     6181 ccggctccgc tgcaggtgag cgtataggtg tatgtgccag attgcgctgc ggtcacgctt
     6241 tgcgatccgg aaagtgcctt cgctcccgac catgcgccag atgcggagca cgaagtcgca
     6301 ttgctgacgt tccaggtgag cgtagaggta ccgccgacag caacggtgtc tggattgggt
     6361 gttagcgaaa tacctggcgg gggtatgcca ttgactgaca cagatttgct ggcgccatcg
     6421 caggctaggg taaaggtatt ggttcctgca tggccgacca agacctgcgc actgccggac
     6481 aatgcacgaa cacccgacca gtcacctgat gccaggcaag acgacgcatt gctcgagttc
     6541 caggtgagcg tcgtgttttg ccccacccgc acactcgccg gacttgcatt gagggaaacc
     6601 tgcgccggag gcagccccgt caccacgacc gactggatgg cggttgcgcc aactgcactg
     6661 caaatgagac tgaaggtgtt gctgccgacc tgcgaaacgg cgaccaaatg ctcaccgttg
     6721 aagggcactt gttcggacca gacgtatccg tctgccacgc agttgctggc accggataca
     6781 tcccaggaga gatcgacgtt gctgcctgcc gtgacctgat agtgatcagc cgtaaggcta
     6841 atagaaggtg cagccggcgc ggggtcggaa accggatcga ggcgatatac cgtacctgaa
     6901 cccacctgtc caaagaaatt gctgatgccg taaaagctgc cattgtcgtc cctcaccagc
     6961 gcggcggcgg acccatcgcc atcgaagtcg gaaaaggtga acagcctcgt caccgtgccg
     7021 tcgaccgcaa g
//
LOCUS       DASZZC010000640         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_565644,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000640 DASZZC010000000
VERSION     DASZZC010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565644"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VHE37_13635"
     CDS             <1..779
                     /locus_tag="VHE37_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HVT36625.1"
                     /translation="ANLAHDSFDHDFAQVLQRARAAGVGTIIITGSDAGSARKALALA
                     REHAGLYATAGLHPHHASDWSADTAALFEQLAQDEKLVALGECGLDYFRDFSPRDVQR
                     SAFAAQLELAARLRRPLFLHQRDAHVDFLPILREHRAALAEVVVHCYTDTRAALAEYL
                     ALDCHIGITGWVCDQRRGSALREIVRDIPDERLLIETDAPYLLPHTAPKSASRRNEPC
                     FLPYVVETLAAQRGQSVEHVARITARNARDFFRLPEPKTS"
     gene            complement(880..2559)
                     /gene="ilvD"
                     /locus_tag="VHE37_13640"
     CDS             complement(880..2559)
                     /gene="ilvD"
                     /locus_tag="VHE37_13640"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVT36626.1"
                     /translation="MSDQLRSRTITAGVSRSPNRAMLRAVGFTDADFDKPIVGIASGH
                     STMNPCNAGLGPLADRAAAAVKAAGGMPQTFGFPTVTDGISMGTEGMKYSLVSREVIA
                     DSIETAAQSQFMDGVLAIGGCDKNMPGAMIALLRMNVPGLFCYGGTIKPGFWKGTALT
                     IVSPFEAVGAFNAGKMSQEDFEGIEKNACPSFGACGGMYTANTMSSAFEALGMSLLGS
                     SQMANPDPEKGDSVAQSAAVLVNAIRKNIRPRDIVTRKSIENSIALVMATGGSTNAVL
                     HFLAIAHAAGVKWSLADFERVRRRTPVLCDLKPSGKYVAVDFHKAGGVPQVLKMLLKA
                     GLLHGDCITITGKTMAEELKNIPDEPRKDQEVIRPMSAPMYRQGHLAILKGNLAPDGA
                     VAKITGLKNPTITGPARVFDSEQAAMTAILADRIKPGDVLVLRYLGPRGGPGMPEMLA
                     PTSAIIGKGLGESVGFITDGRFSGGTWGMVVGHVAPEAFDGGPIALVKNGDSITIDAK
                     KQLIQVNVSARELAKRKKAWKQPKPRYTSGVLGKYARLVSSADKGAVTDAK"
     gene            complement(2620..3162)
                     /locus_tag="VHE37_13645"
     CDS             complement(2620..3162)
                     /locus_tag="VHE37_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36627.1"
                     /translation="MRRAALALLLAVASVGATAQPAARRAQQHPPPAAAPAGCSDAQY
                     RVFDFWGGSWMAISADGSRVLGHSAVLPVLGGCALEQRWTGGTGFHAQSFAAYDREQG
                     LWRLMSADQGGNTLLLQGGWTGTEMSFSGEHTRDGMHYRDRMVFTPLASGHVRQTWES
                     MPDGGNWNSQFEAELQHTRN"
     gene            complement(3159..>3626)
                     /locus_tag="VHE37_13650"
     CDS             complement(3159..>3626)
                     /locus_tag="VHE37_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HVT36628.1"
                     /translation="DFDKRGVKAIGLSVDPLGSHEGWANDIKETQGHALNFPLIADPE
                     RKVSNLYDMIHPNASDTFTVRSVFIIDPNKKIRLTLTYPASTGRNFDEILRVIDSLQL
                     TDSQKLATPVNWKPGDDAIIVPSLKDDDAKKLFPQGWKTLKPYLRLVKPGKPA"
BASE COUNT          560 a         1247 c         1171 g          648 t
ORIGIN      
        1 gcgccaacct cgcgcacgac agcttcgatc acgattttgc gcaggtgctg cagcgcgcgc
       61 gcgccgccgg tgtcggcacc atcatcatca ccggcagcga tgccggttcc gcgcgcaagg
      121 cgctggcgct ggcgcgcgag cacgcggggc tgtacgccac cgcgggcctg cacccgcatc
      181 acgccagcga ctggagcgcc gacactgcgg cgctgtttga gcagctggcg caggacgaaa
      241 aactggtcgc actgggcgaa tgcgggctgg attacttccg cgatttctcg ccgcgcgatg
      301 tgcagcgcag cgctttcgcc gcgcagctcg aactggccgc gcgcctgcgc aggccgctgt
      361 tcctgcacca gcgcgacgcg cacgtggatt tcctgccgat cctgcgcgag catcgcgcag
      421 cgctcgccga agtggtggtg cattgctaca ccgacacgcg cgcggcgctg gcggagtacc
      481 tcgcgctcga ctgccacatc ggcatcaccg gctgggtctg cgaccagcgc cgcggcagcg
      541 cgctgcgcga gatcgtgcgc gacattcccg acgagcgcct gctgatcgaa accgacgcgc
      601 cgtacctgct gccgcacacg gcgccgaaga gcgcatcacg gcgcaacgag ccctgcttcc
      661 tgccctacgt ggtggagacg ctcgctgcgc agcgcggcca gagcgtggag cacgtcgcgc
      721 ggatcaccgc gcgcaatgcg cgggattttt tccgcttgcc ggagcccaaa acgagttgac
      781 cgcaccctgc cttgacgtgt tattaaaact cgtatttatt taatttaata caacaattaa
      841 taacacctta aatggcagtg cggtctgctg tttttttgcc tacttcgcgt cggtgacggc
      901 gcccttgtcc gcgctgctga ccagccgcgc atacttgccc agcacaccgc tggtatagcg
      961 cggcttcggc tgcttccacg ccttcttgcg cttcgccagc tccctggcgg aaacgttgac
     1021 ctggatcagc tgcttcttcg cgtcgatggt gatgctgtcg ccgttcttca ccagcgcgat
     1081 cggcccgcca tcgaacgctt ccggcgccac gtgtccgacc accatgcccc aggtgccgcc
     1141 ggaaaagcgg ccgtcggtga tgaagcccac gctctcgccc aggcccttgc cgatgatggc
     1201 gctggtcggc gccagcatct ccggcatgcc agggccacca cgcgggccga ggtagcgcaa
     1261 caccagcacg tcgccgggct tgatcctgtc ggcgaggatc gccgtcatcg ccgcctgctc
     1321 ggaatcgaac acgcgcgccg ggccggtgat ggtcggattc ttcaggccgg tgattttcgc
     1381 caccgcgccg tcgggcgcaa ggttgccctt gaggatcgcc aggtgcccct gccggtacat
     1441 cggcgcgctc atcggccgga tcacctcctg atccttgcgc ggctcgtcgg gaatgttctt
     1501 cagctcctcg gccatggtct tgccggtgat ggtgatgcag tcaccgtgca gcagcccggc
     1561 cttgagcagc atcttgagca cctgcggtac gccgccggcc ttgtggaaat ccaccgccac
     1621 gtacttgccg ctgggtttca agtcgcacag caccggcgtg cgcctgcgca cgcgctcgaa
     1681 atccgcgagc gaccacttca cgcccgccgc gtgcgcgatg gccaggaaat gcagcaccgc
     1741 gttggtcgag ccgccggtgg ccatgaccag cgcaatggag ttctcgatcg acttgcgcgt
     1801 gacgatgtcg cgcgggcgga tatttttcct gatcgcgttg accagcaccg cggccgactg
     1861 cgccacgctg tcgcccttct ccgggtcggg gttggccatc tgcgaagagc cgagcaggct
     1921 catgcccagc gcctcgaacg cgctcgacat ggtgttggcg gtgtacatgc cgccacaggc
     1981 gccgaagctg gggcaggcgt tcttctcgat gccctcgaaa tcttcctggc tcatcttgcc
     2041 ggcattgaat gcgcccaccg cttcgaacgg cgagacgatg gtcagcgccg tgcccttcca
     2101 gaaaccgggc ttgatggtgc cgccgtagca gaacaggccc ggcacgttca tgcgcagcag
     2161 cgcgatcatc gcgccgggca tgttcttgtc gcaaccaccg atcgccagca cgccgtccat
     2221 gaactgcgac tgcgcggcgg tctcgatcga atcggcgatc acctcgcgcg agaccagcga
     2281 gtacttcatg ccctcggtgc ccatgctgat gccgtcggtc acggtcggga agccgaaagt
     2341 ctgcggcatg ccgccggcag ctttcactgc cgccgccgcg cggtcagcca gcgggccgag
     2401 cccggcgttg cacgggttca tggtcgagtg gccgctggcg atgccgacga tgggcttgtc
     2461 gaaatccgca tcggtgaagc ccaccgcgcg cagcatggcg cggttgggcg agcgggagac
     2521 tcccgcggtg atcgtgcggc tgcgcagctg gtcgctcatg gttgcacccg gcaaaaaaat
     2581 gcccccagga cgggggcgcg tattctacgt tgcgcgcttt caattgcgcg tgtgctgcag
     2641 ttcggcctcg aactgcgaat tccagttgcc gccgtcgggc atgctttccc aggtctgccg
     2701 cacgtgaccg ctggccagcg gcgtgaacac catgcggtcg cggtagtgca tgccatcgcg
     2761 cgtgtgctcg ccgctgaacg acatctcggt gccggtccag ccgccctgca gcagcagcgt
     2821 gttgccgccc tggtcagccg acatcagccg ccacaggccc tgctcgcggt cgtaggcagc
     2881 gaagctctgc gcgtggaagc cggtgccgcc ggtccagcgc tgctccagcg cgcagccgcc
     2941 cagcaccggc agcaccgcgc tgtgcccgag cacgcgcgaa ccgtcggcgc tgatcgccat
     3001 ccagctgccg ccccagaaat cgaacacgcg gtactgcgca tcgctgcaac cagccggcgc
     3061 cgctgccggc ggcggatgct gctgcgcgcg ccgcgccgcg ggctgcgcag tggcgcccac
     3121 cgacgccacc gccagcagca gcgcgagcgc tgcacgcctc atgcgggctt gccgggcttg
     3181 accaggcgca ggtagggctt gagcgtcttc cagccctgcg ggaacagctt cttcgcatcg
     3241 tcgtccttga gcgaaggcac gatgatggcg tcgtcgcccg gcttccagtt caccggcgtg
     3301 gccagcttct ggctgtcggt aagctgcagc gaatcgatca cccgcaggat ctcgtcgaag
     3361 ttgcgcccgg tggacgcggg ataggtcagc gtcaggcgga tcttcttgtt cggatcgatg
     3421 ataaacacgc tgcgcacggt gaaggtgtcg gacgcattcg gatggatcat gtcgtacagg
     3481 ttggagacct tgcgctcggg gtcggcgatc agcgggaagt tgagcgcatg gccctgggtt
     3541 tccttgatgt cgttggccca gccttcgtgc gagccgagcg gatcgaccga aagcccgatc
     3601 gccttcaccc cgcgcttgtc gaagtc
//
LOCUS       DASZZC010000641         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_565690,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000641 DASZZC010000000
VERSION     DASZZC010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565690"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VHE37_13655"
     CDS             complement(<1..708)
                     /locus_tag="VHE37_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HVT36629.1"
                     /translation="MSSRMTAYEKQAAAALRAWAAEPPPLAARWTGRAAGPAGKAVEA
                     AVPGSLLRAVLKGLDRAADRFSGHAALLRAAGVDRAEALRDEASLETCDGLAARVQRR
                     AMVLAGGTGAVLGIAGVAGLVADVPTLIAQALRAVRRIAFCYGEAVTPAELRRLSIGV
                     FALASATSMEEKDAALAALRDGTLALEHAGVRESMERAAQRELAKEAAVLSITNVARS
                     VGRNLGWRRMAAGSLPGL"
     gene            826..1071
                     /locus_tag="VHE37_13660"
     CDS             826..1071
                     /locus_tag="VHE37_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT36630.1"
                     /translation="MIHNRIAVLRTERGLSRKELADAVGVNFQTIGYLERGEYSPSIE
                     LALKLAKHFALPVEMIFSLKPFPALSHELLRSQAAHK"
     gene            1106..1315
                     /locus_tag="VHE37_13665"
     CDS             1106..1315
                     /locus_tag="VHE37_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36631.1"
                     /translation="MNRHFRIYNAILWAIAIIVSALLKAPNELTLLALPTLSISALML
                     ADRAVAAQKPCSAATGADHDPQHDA"
     gene            1293..>1811
                     /locus_tag="VHE37_13670"
     CDS             1293..>1811
                     /locus_tag="VHE37_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36632.1"
                     /translation="MIRNMMRNPVMGSSMRSAHGRRAAVLMIYIGYAVGVYCWLKGEA
                     LGVDWVMGAAVLAFVECAIGVLLLFRWSRYGLWGHMWRWKSEMPLRNWLHGDPQVMKD
                     FDERELAVRDRAHVAAYSIYTTLTMLLFSVYDVALAQRNPPQLSFEQAQLILWGLIVT
                     VLTLPAAILAWSD"
BASE COUNT          279 a          652 c          593 g          287 t
ORIGIN      
        1 caggcccggc aggctgccgg ccgccatgcg ccgccagccg aggttgcggc cgacgctgcg
       61 cgccacgttg gtgatcgaca gcaccgccgc ttccttggcc agctcgcgct gcgccgcgcg
      121 ctccatgctt tcgcgcacgc ccgcgtgctc cagcgccagc gtgccgtcgc gcagcgctgc
      181 cagtgccgca tccttttctt ccattgacgt ggccgaggcc agcgcgaaca cgccgatcga
      241 caggcggcgc agttccgccg gcgtcaccgc ctcgccgtag cagaaggcga tgcggcgcac
      301 cgcgcgcaac gcctgcgcga tcagggtcgg tacatccgcc accagcccgg cgacgccggc
      361 gatgccgagc accgcgccgg tgccgccggc cagcaccatg gcgcggcgct gcacgcgtgc
      421 cgccagcccg tcgcaggttt ccagcgatgc ctcgtcgcgc aaggcttcgg cgcggtcgac
      481 gcccgccgcg cgcagcagcg cagcgtgtcc ggaaaaccgg tccgccgcgc ggtccagccc
      541 cttgagcacc gcgcgcagca gcgagcccgg caccgccgcc tccacggcct tgcccgccgg
      601 acccgcggca cgcccggtcc agcgcgcggc cagcggcggc ggttcggcgg cccaggcgcg
      661 cagtgcggcg gcagcctgct tctcgtacgc agtcatacgc gagctcatgt cggacactct
      721 acctttcgac atcggcgcgc gccggatggc ggaccgattt gacaggcatg catgcctgtt
      781 gtacatatac aacatatagt tgcttaaaca gtacaggacg ttggaatgat tcataaccgc
      841 atcgccgtgc tgcgcaccga acgcgggctc tcgcgcaagg agctggcgga cgcggtcggc
      901 gtgaacttcc agaccatcgg ctatctcgag cgcggcgagt acagccccag catcgagctc
      961 gcgctcaagc tggcgaaaca cttcgcgctg ccggtcgaaa tgattttttc gctgaaacct
     1021 tttcccgcgc tgagccatga gctgctgcgc agccaggccg cgcacaaatg aacgcgtccg
     1081 cggacgcatc tgcaggagag tcgccatgaa ccgccacttc cggatttaca acgccatcct
     1141 ctgggccatc gccatcatcg tctcggcgct gctcaaggcg cccaatgagc tcacgctgct
     1201 ggcgctgccc acgctgtcga tcagcgcgct gatgctggcc gaccgcgcgg tcgcggcaca
     1261 gaagccttgc tcagccgcca ccggagcaga ccatgatccg caacatgatg cgtaacccgg
     1321 tgatgggctc gagcatgcgc agcgcccacg gccgccgcgc ggcggtgctg atgatctaca
     1381 tcggctacgc ggtcggcgtg tattgctggc tcaagggcga ggcgctgggc gtggactggg
     1441 tgatgggcgc cgccgtgctg gccttcgtcg aatgcgccat cggcgtgctg ctgctgttcc
     1501 gctggtcgcg ctacggcctg tggggccaca tgtggcgctg gaagagcgag atgccgctgc
     1561 gcaactggct gcacggcgat ccgcaggtga tgaaggattt cgacgagcgc gaactggcgg
     1621 tgcgcgaccg cgcgcacgtc gccgcctact ccatctacac cacgctcacc atgctgctgt
     1681 tcagcgtgta cgacgtggcg ctggcgcagc gcaatccgcc gcagctgagc ttcgagcagg
     1741 cgcagctgat cctgtggggg ctgattgtca ccgtgctcac cctgcctgcg gccatcctgg
     1801 cctggagcga c
//
LOCUS       DASZZC010000642         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_246505,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000642 DASZZC010000000
VERSION     DASZZC010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246505"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..674
                     /locus_tag="VHE37_13675"
     CDS             78..674
                     /locus_tag="VHE37_13675"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HVT36633.1"
                     /translation="MTPSDVLAFWFGRSSDDAVVATEQGELWFGGDAATDALIREHYG
                     ELREQAIAGQLHEWESSAEGLLALVILVDQFSRNIHRGTAAAFAHDAMARSWARRMVA
                     NGGYARLRPIQRLFVALPLEHSEALADQQQGVALLEQLARQVPPELREKFDSYADYAR
                     RHRDIIARFGHFPHRNRILGRDSTHEEIEFLKQPGSSF"
     gene            complement(754..1869)
                     /locus_tag="VHE37_13680"
     CDS             complement(754..1869)
                     /locus_tag="VHE37_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HVT36634.1"
                     /translation="MDRSDAGAERSAEVLAAAAIGDAREAGLRYVRADQPGLSRLRAG
                     RAFRYIDAQGRAVNDAATLARIRRLAIPPAYQRVWICADARGHLQATGYDARGRKQYR
                     YHADWRAARDGSKFARMVEFGERLPRLRRRVARDLKLPALPRDKVLATVVAVLDATRV
                     RIGNDEYARANRSYGLTTLRNRHVDFIRDGRARFTFRGKGGAVHDVVLDDRRLSAIVR
                     RCQQLPGQRLFQYVDDDGAVRAIDSGDVNDYLRDAMGADFTAKDFRTWGATLSAIARM
                     ARTPLPSCERDFKSCIADAVREVAAELRNTPAVCRKSYINPLVFSAWRDGTLHRLLGN
                     AAPAATRRAEAAALAFLRAAARPLPMSRRLRKRATAC"
     gene            1975..3108
                     /locus_tag="VHE37_13685"
     CDS             1975..3108
                     /locus_tag="VHE37_13685"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HVT36635.1"
                     /translation="MQARTRRWLLRGTWTLVAIMLLLGAALWIGAQELRGRVAAMLGP
                     GATIGAMYAGPTGLLLEDVHMPAPPGWPAGDAASVQSVRVVPELRSLFSGTPRMRSIE
                     VQQAQLVLMRSRAGIQLLPGVVHAGDGGEHGPARGADLSSVTLHHSTLIWVDQTVPHP
                     PLTLELRDLDIAIADLDVPALQQHTQVRADGTLHGPRHDGAVHVEGWIQLATLDSDLK
                     LRLHQVDLDSVQPYLERDPAQGAQHGLLDLDTESRIDRRRLRAPGSIRLSQLSFSPGA
                     SSFLGAPRAATLKYFEQAHDHLDLNYVVEGDLDNPKFSLNDSIATKVALAAGETLGVS
                     IVDVAKGVTTLGSRSVDAVKGIGSGVGHLLGIAGERKKDDERK"
     gene            3171..>3420
                     /locus_tag="VHE37_13690"
     CDS             3171..>3420
                     /locus_tag="VHE37_13690"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HVT36636.1"
                     /translation="MGFPRWILLLGYAGLIPFAAGPLILTFAPEHAPARLDHLWLLYA
                     GLIASFMSGTFWGFSLPAVQGEEGMTGIVIASLLMIATW"
BASE COUNT          504 a         1182 c         1188 g          546 t
ORIGIN      
        1 gtgacgctca cgctgctgct gctcctggtc ggcggtgcgc tgtgcatctg gcaggggctg
       61 cgcggcatgg ccttgctgtg acgccctccg acgtcctcgc cttctggttc gggcgcagca
      121 gcgacgatgc ggtggtcgct actgagcaag gtgagctctg gttcggcggc gacgccgcca
      181 ccgatgcgct gatccgcgag cactacggcg agctgcgcga gcaagccatc gccgggcagt
      241 tgcacgaatg ggaaagcagc gccgagggtc tgctcgcgct ggtcatcctg gtggaccagt
      301 tctcgcgcaa catccaccgc gggacggccg cagcgtttgc ccacgacgcc atggcgcgca
      361 gctgggcgcg gcggatggtg gccaacggcg gctacgcacg gctgcgcccg atccagcgcc
      421 tgttcgtcgc cctgccgctg gaacattccg aagcgctggc cgaccagcag cagggcgtgg
      481 cgctgctcga gcagctggca cgccaagtgc cgccggagct gcgcgaaaaa ttcgacagct
      541 atgccgacta cgcgcggcgc caccgcgaca tcattgcgcg cttcggccat tttccccatc
      601 gcaaccgcat cctcggccgt gactccaccc acgaggaaat cgagttcctc aagcagcccg
      661 gatcgtcgtt ttagccggca ccgcactgct atttggcgtg ttataaaaag ttatatattc
      721 ttaaataaga taaataattt ataacatcaa aaatcagcat gcggtggcac gtttccgcag
      781 ccgccgcgac atcggcagcg gcctggcggc ggcccgcagg aatgccagcg ccgccgcttc
      841 ggcccggcgc gtcgccgccg gcgccgcgtt gccgagcagc cggtgcagcg tgccgtcgcg
      901 ccaggcgctg aacaccagcg ggttgatgta cgacttgcgg cacaccgccg gcgtgttgcg
      961 cagttccgcc gccacttcgc gcacggcatc ggcgatgcag ctcttgaagt cgcgctcaca
     1021 cgagggcagc ggcgtgcgcg ccatgcgcgc gatggccgac agcgtggcgc cccaggtgcg
     1081 gaaatccttg gcggtgaaat ccgcgcccat ggcgtcgcgc aggtaatcgt tgacgtcgcc
     1141 cgaatcgatg gcgcgaaccg cgccgtcgtc gtccacgtac tggaacaggc gctgcccggg
     1201 cagctgctgg cagcggcgca cgatggccga caggcgtcta tcgtcgagca ccacgtcgtg
     1261 aaccgcgccg cccttgccgc gaaaggtgaa gcgcgcgcgc ccgtcgcgga tgaaatccac
     1321 gtggcggttg cgcagcgtgg tcaggccata ggagcggttg gcgcgcgcgt actcgtcgtt
     1381 gccgatgcgc acgcgggtgg cgtcgagcac cgcgaccacg gtggccagca ccttgtcgcg
     1441 cggcagcgcc ggcagtttca ggtcgcgcgc cacccggcgg cgcaggcgcg gcagccgttc
     1501 gccgaattcg accatgcggg cgaacttgct gccgtcgcgc gcggcgcgcc agtcggcgtg
     1561 gtagcggtac tgcttgcgcc cgcgcgcgtc gtagccggtg gcctgcagat ggccgcgcgc
     1621 atcggcgcag atccacacgc gctggtacgc cggcggaatc gccagccggc ggatgcgcgc
     1681 cagcgtggcg gcgtcgttca ccgcgcggcc ctgcgcgtcg atgtagcgga atgcgcgccc
     1741 ggcgcgcagg cgcgacaggc ccggctggtc ggcgcgcacg tagcgcagcc cggcttcgcg
     1801 tgcgtcgccg atcgccgctg ccgccaggac ttcggcgctg cgctccgcac ctgcatcgct
     1861 gcgatccatg tccgcttggg catggagcgg gcgggcaaag ttcgcgcggc cgctgaaatc
     1921 gcgcgcggcg gcgcggcata ctgcgcggca attttttccg tcaacttctt ttttatgcag
     1981 gcaaggacaa gacgctggct gctgcgtggc acctggactc tcgttgccat catgctgctg
     2041 ctcggcgccg cgctgtggat cggcgcgcag gaactgcgcg ggcgcgtggc ggcgatgctc
     2101 ggccccggag ccaccatcgg cgccatgtat gccggcccca ccgggctgct cctggaggac
     2161 gtgcacatgc cggcgccgcc gggctggccc gcgggcgatg cggccagcgt gcaatcggtg
     2221 cgcgtggtgc cggagctgcg ttcgctgttc agcggcacgc cgcgcatgcg cagcatcgag
     2281 gtgcagcagg cgcagctggt gctgatgcgc agcagggcgg gcatccagct gctgcccggt
     2341 gtcgtgcatg ccggcgatgg cggcgagcac ggccctgccc gcggcgccga cctgtcgagc
     2401 gtcacgctgc accacagcac gctgatctgg gtggaccaga ccgtgccgca tccgccgctg
     2461 accctcgagc tgcgcgatct cgacatcgcc atcgccgatc tcgacgtgcc ggcgctgcag
     2521 cagcacacgc aggtgcgcgc cgacggcacg ctgcacggcc cgcgccacga cggcgcggtg
     2581 catgtcgaag gctggatcca gctggccacg ctcgattccg atctcaagct gcgcctgcac
     2641 caggtggacc tggacagcgt ccagccatac ctggagcgcg accccgcgca gggcgcgcag
     2701 cacggactgc tcgacctcga taccgagtcg cgcatcgacc ggcgccggct gcgcgcgccc
     2761 ggctccatcc gcctgagcca gctcagcttc tctccgggtg ccagttcgtt cctgggcgcg
     2821 ccgcgcgcgg ccacgctgaa gtatttcgag caggcgcacg atcacctcga cctgaactac
     2881 gtggtcgaag gcgacctgga caatccgaag ttttcgctca acgacagcat cgccaccaag
     2941 gtggcgctgg ctgccggcga aacgcttggt gtatccatcg ttgacgtggc caagggcgtc
     3001 accacgctgg gcagcaggag cgtggacgcg gtgaagggca tcggcagcgg cgtcggtcat
     3061 ctgctgggca tcgccggtga gcggaaaaag gacgacgagc gcaagtagcg ctgcctcggc
     3121 gccgcggttt gggtgcacaa tcgccgcatc ggtgacgtcg agggggcatc atgggtttcc
     3181 cgcgctggat cctgctgctg ggttacgccg gcctgattcc gttcgccgcc ggcccgctga
     3241 tcctcacctt cgccccggag cacgcgccgg cgcggctgga ccacctgtgg ctgctctacg
     3301 ccgggctcat cgccagcttc atgtccggca ccttctgggg cttctcgctg ccggcggtgc
     3361 agggcgagga aggcatgacc ggcatcgtca tcgcgtcgct gctgatgatc gccacctggg
//
LOCUS       DASZZC010000643         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_747806,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000643 DASZZC010000000
VERSION     DASZZC010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747806"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VHE37_13695"
     CDS             complement(<1..1829)
                     /locus_tag="VHE37_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVT36637.1"
                     /translation="MAEQETRRVGRTGRETALLFMTIVFFLVAIAAAAAYYTTTTRNQ
                     TWLDQSRQLPLDVAELAKVGEDATRGIAPDFQLLSGRSQDVGDNVRFLREGDAESGIK
                     AMPSSVGQQVAAVEESWRAMKQAIDGVLAGEIPFRRTSTNIAAIAEAAPALITAYEQN
                     ADRLARSGAPAGQVFLAGSQVARLERIVASARNVTSQGRDAVASAAALDQEVKAFQKI
                     GASADFETIATAAAAIVQDAEAVQKMQVSAATLQGAGTNVLATAKSLEQGLLDTVKVY
                     TFAPMAAAFSAVIALLSLGLYIASTFRSVSDRQRAAEDRDARQQQAILGLLDEITNLA
                     DGDLTVDVTVTEDFTGAIADSINYTVGNMRNLVGTINSTSVELAGAATSTQDIALKMN
                     EASERQAKEVAAVANTITATSQSLQQVAQRAEQLAQQARGSVDVAHAGAATVGRTIQG
                     MAALREQIQDTSKRIKRLGESSQEIGNIIEFINDIAEQTNTLALNASIQAAMAGEAGR
                     GFAVVADEVQRLAERAATATRQIENLVKTIQADTNEAIVSMERSTTNVVTGAKSAEEA
                     GQALTKIETSSQELAKLITEISASARNQSAAATKIAGTMQVIR"
     gene            complement(1862..2227)
                     /locus_tag="VHE37_13700"
     CDS             complement(1862..2227)
                     /locus_tag="VHE37_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT36638.1"
                     /translation="MARIMIVDDSPTDVENLRGMLTKAGHSVVECTSGQDCIARVKAE
                     KPNAVIMDVVMPGVNGFQATRMLSKDPDTSNIPVIIVSSKNQETDRVWALRQGAREYL
                     TKPVKDADLIAKINSVLGG"
     gene            complement(2358..>2676)
                     /locus_tag="VHE37_13705"
     CDS             complement(2358..>2676)
                     /locus_tag="VHE37_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT36639.1"
                     /translation="PLTEPGISALETLLPLALELVHDKLLTPLQLAQRLSLGPADVLG
                     IDGGRLAAGRPANLVLVDPDRRWTLQTEEMLSAGRNTPFGGRRFRGRAVRTLAQGLGV
                     TPR"
BASE COUNT          356 a          868 c          938 g          514 t
ORIGIN      
        1 tcacggatga cctgcatggt gccggcgatc ttggtcgctg cagccgactg gttgcgcgcg
       61 ctggcggaaa tctcggtgat cagcttggcc agttcctgcg agctggtctc gatcttggtc
      121 agtgcctggc ccgcttcttc ggcactcttc gcaccggtga ccacgttggt ggtggagcgc
      181 tccatcgaga cgatggcctc gttggtgtcg gcctgaatgg tcttgaccag gttttcgatc
      241 tggcgcgtcg ccgtggcggc gcgctcggcg agtcgctgca cttcgtccgc gaccaccgcg
      301 aagccgcggc ctgcttcgcc ggccatggcg gcctggatcg atgcgttgag cgccagcgtg
      361 ttggtctgtt cggcgatgtc gttgatgaac tcgatgatgt tgccgatttc ctgcgaggac
      421 tcgcccaggc gcttgatgcg cttggaggtg tcctggatct gctcgcgcag cgcggccatg
      481 ccctggatgg tgcggcccac cgtcgcggcg ccggcgtgcg ccacgtcgac cgagccgcgc
      541 gcctgctggg ccagttgctc ggcgcgctgc gccacctgct gcagcgactg cgaagtggcg
      601 gtgatggtgt tggccaccgc ggccacttcc ttggcctggc gttccgaggc ctcgttcatc
      661 ttcagcgcga tgtcctgcgt gctggtggcg gcgccggcga gttcgaccga cgtcgagttg
      721 atcgtgccga ccaggttgcg catgttgccg acggtgtagt tgatcgagtc ggcgatggcg
      781 ccggtgaagt cttccgttac cgtcacgtcc accgtgaggt caccgtcggc caggttggtg
      841 atttcgtcga gcaggcccag aatcgcctgt tgctggcgcg cgtcgcggtc ttcggcggcg
      901 cgctggcggt cgctgacgct gcggaacgtg gaggcgatgt acaggcccag cgagagcagg
      961 gcgatgacgg cggaaaatgc cgcggccatt ggcgcgaagg tgtagacctt gaccgtgtcg
     1021 agcaggccct gttccaggct cttggcggtg gccagcacgt tggtgccggc gccctgcagc
     1081 gtggctgccg acacctgcat cttctgcacc gcctcggcgt cctgcacgat ggcggcggcg
     1141 gcggtggcga tggtctcgaa atcggcgctg gcgccgatct tctggaaggc tttcacttcc
     1201 tggtccagcg cggccgcgga ggcgacggca tcgcggccct gcgaagtcac gttgcgcgca
     1261 ctggcgacga tgcgctcaag ccgggcgacc tggctgccgg cgagaaacac ctggccggcg
     1321 ggcgcaccgc tgcgcgccag gcggtcggca ttctgctcat aggccgtgat cagcgccggc
     1381 gccgcttcgg cgatggcggc gatattcgtg ctggtgcggc ggaacgggat ttcgccggcg
     1441 agcacgccgt cgatggcctg cttcatcgcg cgccacgatt cctccaccgc cgccacctgc
     1501 tggccgacgg aggagggcat ggccttgatg ccggattcgg catcgccctc gcgcaggaag
     1561 cgcacgttgt cgcccacgtc ctgcgaacgg ccggacagca gctggaaatc cggcgcgatg
     1621 ccgcgggtgg cgtcttcgcc caccttggcc agttcggcca cgtcgagcgg cagctggcgc
     1681 gactggtcga gccaggtctg gttgcgcgtc gtggtggtgt agtacgcggc ggcggcggcg
     1741 atggccacca ggaagaacac gatggtcatg aacagcagcg cggtttcgcg gcccgtcctg
     1801 ccgacgcgtc gggtttcttg ctccgccatg tctcccctca acctctagtt ctattgctgc
     1861 ttcagccgcc gagcaccgaa ttgatcttgg cgatcaggtc ggcatccttc accggcttgg
     1921 tcaggtattc gcgcgcgccc tggcgcagcg cccacacccg gtcggtttcc tggttcttgc
     1981 tgctgacgat gatcaccggg atgttgctgg tgtcggggtc cttggacagc atgcgcgtgg
     2041 cctggaagcc gttcacgccc ggcatgacca catccatgat caccgcgttg ggcttttccg
     2101 ccttgacccg ggcgatgcaa tcctggccgc tggtgcattc caccacgctg tggccggcct
     2161 tggtcagcat gccccgaaga ttctcgacgt cggtgggaga atcatccacg atcatgatgc
     2221 gcgccatagt tgtcgctccc ctttagctgt tgcgtgtacc gggctgatgc gccgcgaatt
     2281 cccggcgggc tgaggcccga tcaaacgtcg cgggagacta tacaccaagc gcgtcgcggg
     2341 caagccgcgg gcagccctca gcgaggggtg acgccgaggc cttgcgcaag cgtgcgtacg
     2401 gcgcgcccgc ggaagcgccg tccgccgaat ggcgtgttgc gtccggcact gagcatctct
     2461 tcggtctgca gggtccagcg ccggtcggga tcgaccagca ccaggtttgc cggacgtccc
     2521 gcggcgagcc gtccgccgtc aatgccgagc acgtcagccg ggcccagcga caggcgctgg
     2581 gccagttgca gcggcgtgag cagcttgtcg tgtaccagct ccagcgccag cggcagcagc
     2641 gtctccagcg cggaaatgcc cggctcggtc agcggg
//
LOCUS       DASZZC010000644         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_819683,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000644 DASZZC010000000
VERSION     DASZZC010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819683"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VHE37_13710"
     CDS             <1..670
                     /locus_tag="VHE37_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HVT36640.1"
                     /translation="LVTALMNAAVAVFIYTLVPEFFMRFIVWMLIHLLYRVRARGLEQ
                     VPDEGPCVVVCNHVSFVDALIVGGSIRRPVRFVMYHKIFRLPLLGFIFRTARAIPIAP
                     KKEDEALMIRAFDEIDAALAAGDVVGIFPEGAITRDGSIDQFRPGVERVLARRPVPVV
                     PMALRGLWGSIFSRRDDFLRRARLPRRFRAQIELVAGPLVQPAQASAEYLEQQVRALR
                     GDLA"
     gene            749..1387
                     /locus_tag="VHE37_13715"
     CDS             749..1387
                     /locus_tag="VHE37_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36641.1"
                     /translation="MGRRNQHGREEQRDMAIAAAELILVKGGYAALSMRKVADAIGYT
                     VGNLYLLFKNQDELLDTINGRTADAIYARLSEAASAAGTQPLARVRAIAAGYIAYAQQ
                     HPHRFRLLFEHQGSTPRTPADADTRVQMFFDLVESSIAPLLPRVRPERLRMAAATLWS
                     GVHGICMLAVTGKLRWSGIEEFRPLSDMLVGTFLAGLGSSPAPRPPPAKKKK"
     gene            complement(1391..>1651)
                     /locus_tag="VHE37_13720"
     CDS             complement(1391..>1651)
                     /locus_tag="VHE37_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36642.1"
                     /translation="GYDTRALAWWSALAALLLLASRRWSDPPSIGRGIRPVNINCVYG
                     LSDQQPQRWIPAWLWLALQIFAAPLVFYWPAHWLLQRLPPPG"
BASE COUNT          252 a          573 c          570 g          256 t
ORIGIN      
        1 cctggtcacc gcgctgatga acgccgcggt ggcggtgttc atctacacgc tggtgcccga
       61 gttcttcatg cgcttcatcg tgtggatgct gatccacctg ctgtaccgcg tgcgcgcgcg
      121 cggactcgaa caggtgccgg acgaggggcc gtgcgtggtg gtgtgcaacc acgtgagttt
      181 cgtcgacgcg ctgatcgtcg gcggcagcat ccgccggccg gtgcgcttcg tcatgtacca
      241 caagatcttc cgcctgccgc tgctcggctt catcttccgc accgcgcgcg cgattcccat
      301 tgcgccgaaa aaggaagacg aggcgctgat gatccgcgcg ttcgacgaga tcgatgccgc
      361 gctcgccgcg ggtgacgtgg tcggcatctt cccggaaggc gccatcacgc gcgacggcag
      421 catcgaccag ttccgccccg gggtggagcg cgtgctggcg cgccgcccgg tgccggtggt
      481 gccgatggcg ctgcgcggcc tgtggggcag catcttctcg cgccgcgacg acttcctgcg
      541 gcgcgcacgg ctgccgcggc gtttccgcgc gcagatcgaa ctggtcgcag gcccgctggt
      601 gcagcccgcg caggccagcg cggagtatct cgagcagcag gtgcgcgcgc tgcgcggcga
      661 tctggcctga gagccgcacc tcaccttttc agatgaatgg acgccggcgt gcgccgacgt
      721 agaatgccgg tccgcacagg gatctgacat gggtcgccgc aaccagcacg gacgcgaaga
      781 gcagcgcgac atggccatcg ccgcggccga actgatcctg gtcaagggcg gctacgcggc
      841 gctgtcgatg cgcaaggtgg ccgatgccat cggctacacc gtcggcaatc tctacctgct
      901 gttcaagaac caggacgagc tgctcgacac catcaacggg cgcaccgccg atgccatcta
      961 cgcgcggctg tcggaggcgg cctcggccgc cggcacgcaa ccgctggcgc gcgtgcgcgc
     1021 gatcgcggca ggctacatcg cctacgcgca gcagcatccg caccgcttcc gcctgctgtt
     1081 cgagcaccag ggcagcacgc cgcgcacgcc ggccgatgcc gatacgcggg tgcagatgtt
     1141 tttcgacctg gtggaatcgt ccatcgcgcc gctgctgccg cgcgtgcgcc ccgagcgcct
     1201 gcgcatggcc gccgccacgc tgtggagcgg cgtgcacggc atctgcatgc tagcggtgac
     1261 cggcaagctg cgctggagcg gcatcgagga attccgtccg ctgtcggaca tgctggtggg
     1321 cacgtttctc gccggcctcg gttcttcgcc ggcgccgcgt ccgccgccgg cgaagaagaa
     1381 gaaataacgc tcagccgggc ggcggcagcc gctgcagcag ccagtgcgcc ggccagtaga
     1441 acaccagcgg cgcggcgaaa atctgcagcg ccagccataa ccacgcgggt atccagcgct
     1501 gcggctgctg gtccgacaat ccatagacgc agttgatgtt caccggccgg atgccgcggc
     1561 cgatcgaggg cggatcgctc cagcggcggc tggccagcag cagcagcgcg gcgagcgcgc
     1621 tccaccaggc cagtgcacgc gtgtcgtagc c
//
LOCUS       DASZZC010000645         6257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_566067,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000645 DASZZC010000000
VERSION     DASZZC010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566067"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /gene="ccmI"
                     /locus_tag="VHE37_13725"
     CDS             complement(<1..497)
                     /gene="ccmI"
                     /locus_tag="VHE37_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HVT36643.1"
                     /translation="MILYLSMAALLIAAAALATRPWWRRGADARVQRRAANVAAYRTR
                     VAEIETDLGNGLIDAESAQELKQELGARLLADADAGADVSTPTAAGKGRGLALIALAL
                     LGAFAALMYYRGDSWRTREMIELAQRDPAQAQALMVQSMVSKLAARLQQNPDDAEGWA
                     MLGRS"
     gene            complement(494..904)
                     /locus_tag="VHE37_13730"
     CDS             complement(494..904)
                     /locus_tag="VHE37_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HVT36644.1"
                     /translation="MLFSVAQAAELDSAQTRRYQALTQELRCLVCQNQNIADSEAPLA
                     ADLRNQVKAQIIAGRSNDEIKQYLTDRYGDFVLYQPPVKSTTWLLWFGPFALVLVALV
                     VALRHIRATHAPRAAARDAAADAAALQKLLDEQK"
     gene            complement(1000..1506)
                     /locus_tag="VHE37_13735"
     CDS             complement(1000..1506)
                     /locus_tag="VHE37_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HVT36645.1"
                     /translation="MRFLIPIVVLVALVGLLYAGLHHDPREVPSPLIGKAAPAFDLPL
                     LDGAPHYSVADLKGRPVLVNYFASWCEGCQVEHPYLLQLAQQEGVEIVGMDYKDEPDA
                     GRAWLARHGNPYKTVVLDHEGTAGLDWGVYGVPETFVLDAAGTIVHKQIGPMTPEAWA
                     QIRPLLKP"
     gene            complement(1507..3495)
                     /locus_tag="VHE37_13740"
     CDS             complement(1507..3495)
                     /locus_tag="VHE37_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HVT36646.1"
                     /translation="MIPELGHFALILALAVALMQVCVPAYGLWKRDARALAVATTAAQ
                     AQFFLVATAYVCLTWCFVQHDYSVAYVAANSHSELPLHYRITAVWGAHEGSVLLWVTI
                     LACWTLAVSVFNRRLPADLSATALAVLGAISVGFLAFIQFTSDPFARNFPVPDNGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAFAYSIAALISGRLDAAWARWVRPWTTTAWMFL
                     TLGITLGSWWAYNELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGLLKSWTV
                     LLAILAFALSLLGTFLVRSGVLVSVHAFASDPARGIFILCFLSIVLGIAFTLYAWRAP
                     KLATAGELNLFSREGLLLINNVFLVVAAASVLLGTLYPLVVDSLKLGKISVGPPYFNS
                     VFVPLFAPLVLLVGLAAVLPWKRGRWSDVVHVLRMPFAAALVLGVLIPLVTVGEVKPA
                     VAAGAWLGCWAMLTAGIEVWRRVRAKRALSDGLASVPRGAWGMALAHFGLGLWALGVT
                     VVSGHSIEKDTALAPGQGADIGAFTFTLLQVQRGQGPNYKSDIGSIAVERAGRRIADL
                     HPEKRQYDSTQQVMTESAVDVTPLRDLYVSLGEPLDGGGWSVRLYFKPMMHLVWLGGV
                     LMFLGGLLAATDRRYRLAVGAGLVRDQGSDRAPGALLQ"
     gene            complement(3503..4087)
                     /gene="ccmE"
                     /locus_tag="VHE37_13745"
     CDS             complement(3503..4087)
                     /gene="ccmE"
                     /locus_tag="VHE37_13745"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HVT36647.1"
                     /translation="MTKRQRRMAAVASLLVGLSAAAALGVTAFRKNMMYFYTPSDLVT
                     GSAPAGAKLRLGGLVERGSVQRGADLLVKFTLSDCEKSVPVQYRGILPDLFREGQGIV
                     ATGAMGGDGVFTADEVLAKHDENYMPPQLASSLKTADGKHSCMPFKRVSDASQKPPLA
                     EADVPRPALRAEGRASGGAVGGVRAARSADVKVT"
     gene            complement(4084..4230)
                     /gene="ccmD"
                     /locus_tag="VHE37_13750"
     CDS             complement(4084..4230)
                     /gene="ccmD"
                     /locus_tag="VHE37_13750"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HVT36648.1"
                     /translation="MSEGFFHMGGYAFYVWGSFGLAALVYGWNALAWRAQRRDALKSL
                     VEAP"
     gene            complement(4223..4969)
                     /locus_tag="VHE37_13755"
     CDS             complement(4223..4969)
                     /locus_tag="VHE37_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HVT36649.1"
                     /translation="MNWVWFHKLASPPSFFSFAEKLAPWLGWSALALIAYGSYGGLVL
                     APADYQQGEAFRIIYVHVPAAALSLSIYTLMAVAGAIAVIWRIKLAECVLIAAAPVGA
                     SFTALALATGMLWGKPMWGAWWVWDARLTSELILLFLYLGVIGLDQAIDDRRAGARAC
                     GLLAMIGVINVPIVHYSVDWWNSLHQGSTILKLGQPNISADMARPLYAMLLGTLLLVA
                     HAVLRRTQNEVLLRERGAGWVREFVGAADV"
     gene            complement(4977..5639)
                     /gene="ccmB"
                     /locus_tag="VHE37_13760"
     CDS             complement(4977..5639)
                     /gene="ccmB"
                     /locus_tag="VHE37_13760"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HVT36650.1"
                     /translation="MSAFAATLARELTLAARNKLQWLMPPLFYVIVVTLFGLGAKAND
                     PLLTAFAPAVLWVGALLSALLSLEVLFRADFEDGTLEHWFLADSALAIVYAKLAAHWL
                     LSGLPLVLLAAPLAGALALPGAGLPVLLAGLALGTPVLSLVGGFAAALTVGLPRGGAL
                     LPVLVLPLITPVVIFGAGAVRAAQSGLPVDAPLYFLAAIFVLSLTVIPWASTAALRNS
                     YE"
     gene            complement(5636..6208)
                     /gene="ccmA"
                     /locus_tag="VHE37_13765"
     CDS             complement(5636..6208)
                     /gene="ccmA"
                     /locus_tag="VHE37_13765"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HVT36651.1"
                     /translation="MPILSAQRLSVARGERVLLKDFSLTLEPGALVHLRGANGAGKTS
                     LLETLAGLRRPAAGEVARPDALHWIGHRNALNLALSPLENLRWWCAINALDRSAVQPA
                     LEQLGVGSLRHRACRTLSAGQKRRSALARLVASKRPLWLLDEPLDGLDADGLALIAQL
                     LSAHLSGGGAAMVTSHQPLPAQLPRREVTL"
BASE COUNT         1105 a         2215 c         2095 g          842 t
ORIGIN      
        1 tacgagcggc cgagcatggc ccagccctcg gcgtcgtcag ggttctgctg cagccgcgcg
       61 gcgagcttgc tcaccatgga ctgcaccatc agcgcctgcg cctgcgccgg atcgcgctgc
      121 gccagttcga tcatctcgcg cgtgcgccag ctgtcgccgc ggtaatacat gagggcagcg
      181 aaagcgccga gcaaggccag cgcgatcagt gccaagcccc gccccttgcc tgcagcggtc
      241 ggggtggaaa cgtcggcgcc ggcatcggca tcagccagca ggcgcgcgcc gagttcctgc
      301 ttcagctcct gcgcggactc tgcatcgatc agcccattgc ccagatcggt ttcaatttcc
      361 gccacgcgcg tgcggtaggc agcgacattg gccgcgcgcc gctgcacgcg cgcgtctgcg
      421 ccgcgccgcc accagggccg cgtcgccagc gccgccgcgg cgatcagcag cgcggccatc
      481 gagagataca ggatcatttc tgctcgtcga gcaatttctg cagggccgcc gcgtcggccg
      541 cagcatcgcg cgccgcggcg cgcggggcat gcgtggcgcg gatgtggcgc agcgcgacga
      601 ccagcgcaac cagcaccagc gcgaacggcc cgaaccaaag cagccacgtg gtcgatttca
      661 ccggcggctg gtacagcacg aaatcgccgt agcggtcggt gaggtattgc ttgatctcgt
      721 cgttgctgcg gccggcgatg atctgcgcct tcacctggtt gcgcaggtcg gccgccagcg
      781 gcgcctccga gtcggcgatg ttctggttct ggcacaccag gcagcgcagt tcctgcgtca
      841 gcgcctggta gcgccgggtc tgcgcgctgt cgagttccgc cgcctgcgcc acagagaaca
      901 acaacgtcag ccccgcgcag gcggggatcc agttcctgcc ggcgttgtga agcaaatcag
      961 aactggattc ccgcctgcgc gggaatgacg gatgctggtt catggcttga gcagcggcct
     1021 gatctgcgcc cacgcttccg gcgtcatcgg cccgatctgc ttgtgcacaa tcgtccccgc
     1081 ggcgtcgagc acgaaggttt ccggcacgcc atacacgccc cagtcgagac cggcggtgcc
     1141 ttcgtgatcc agcaccacgg tcttgtacgg atttccgtgg cgcgcgagcc aggcgcggcc
     1201 ggcatcgggc tcgtccttgt aatccatgcc gacgatctcc acgccctcct gctgcgccag
     1261 ctgcagcagg tacgggtgct ccacctggca gccctcgcac caggaggcga agtagttcac
     1321 cagcacggga cgtcccttga gatcggccac ggaataatgc ggcgcgccat cgagcagcgg
     1381 caggtcgaac gccggcgcgg ccttgccgat cagcgggctc ggcacttcgc gcggatcatg
     1441 gtgcaggccg gcgtagagca ggccgaccag cgcgacgagg accacgatgg ggatgagaaa
     1501 acgcatttac tgcaggagcg cgcctggcgc gcgatccgag ccttgatcgc ggacaaggcc
     1561 cgctcctacc gcaagccggt agcgccggtc cgtcgccgcc agcaggccgc cgagaaacat
     1621 cagcacgccg cccagccaaa ccaggtgcat catcggcttg aagtacaggc gcacgctcca
     1681 gccgccgccg tcgagcggct cgcccagcga cacgtagaga tcgcgcagcg gcgtcacgtc
     1741 gaccgcgctc tcggtcatca cctgctgcgt ggaatcgtac tggcgttttt ccgggtgcag
     1801 gtcggcgatg cgccgccccg cgcgctccac cgcgatgctg ccgatgtcgg acttgtagtt
     1861 cggcccctgc ccacgctgca cctgcagcag ggtgaaggtg aaggcgccga tgtccgcccc
     1921 ctgccccggc gccagcgcgg tgtccttctc gatcgagtgc ccgctgacca cggtgacgcc
     1981 cagcgcccac aggccaagac cgaagtgcgc cagcgccatg ccccaggcgc cgcgcggcac
     2041 gctggccagg ccatcgctga gcgcacgctt cgcgcgcacg cgccgccaga cttcgattcc
     2101 cgccgtgagc atggcccagc agccgagcca ggcgccggcc gctaccgccg gcttcacctc
     2161 accgaccgtg accagcggga tcagcacgcc gagcaccagc gcggcggcga agggcatgcg
     2221 caacacgtgc accacgtcgc tccagcgccc gcgcttccac ggcagcaccg cggccagtcc
     2281 caccagcagc accagcggcg cgaacagcgg cacgaacacg ctgttgaagt acggcgggcc
     2341 gacggagatt tttcccagct tcagggagtc gaccaccagc gggtaaagcg tgccgagcag
     2401 cacgctggcc gcggccacca ccaggaatac gttgttgatc agcagcaggc cttcgcgcga
     2461 aaaaagattc agctcgccgg cggtggccaa tttgggcgcg cgccaggcgt acagcgtgaa
     2521 ggcgatgccg agcacgatgc tgagaaagca caggatgaag atgccgcgcg ccggatcgga
     2581 ggcgaacgca tgcaccgaca ccagcacgcc cgagcgcacc aggaacgtgc cgagcagcga
     2641 cagcgcgaag gccaggatcg cgagcagcac ggtccacgat ttcagcagcc cgcgtttttc
     2701 cgtgacagcg agcgaatgga tcagcgcggt gccgaccagc cagggcatga acgaggcgtt
     2761 ctccaccggg tcccagaacc accagccgcc ccagccgagc tcgttgtacg cccaccacga
     2821 gcccagcgtg atgcccagcg tgaggaacat ccatgccgtc gtggtccatg gccgcaccca
     2881 gcgcgcccag gcggcgtcga gccggccgga aatgagcgcg gcgatcgagt aggcgaaggc
     2941 cacggaaaat ccgacgtagc ccatgtagag catcggcggg tggatcacca tgcccgggtc
     3001 ctgcagcagc gggttgagat cgcggccgtt gtccggcacc gggaagttgc gcgcaaacgg
     3061 atcggaggtg aactggatga aggcgaggaa gccgacgctg atcgcgccga gcacggccag
     3121 cgcagtggcg gaaagatccg ccggcagccg ccggttgaac accgacacgg ccagcgtcca
     3181 gcacgccagg atggtcaccc agagcagcac cgagccttcg tgcgcacccc acaccgcggt
     3241 gatgcggtag tgcagcggca attccgaatg cgaattcgcc gccacgtagg ccaccgagta
     3301 atcgtgctgc acgaagcacc aggtcaggca cacgtacgcg gtggcgacga ggaagaactg
     3361 cgcctgcgcg gcggtggtgg cgacagcgag cgcgcgcgcg tcgcgcttcc acagaccata
     3421 cgcgggcacg cacacctgca tcagcgcaac agccaatgca agaatcagcg cgaagtggcc
     3481 gagttcggga atcacattgc tctcacgtga ccttcacgtc tgcgctgcgc gcagccctca
     3541 ccccgccaac ggctccgccg ctggccctcc cctctgcccg cagcgcgggg cgagggacat
     3601 cggcctccgc aagcggcggt ttctgactcg cgtcagaaac tcttttgaag ggcatgcacg
     3661 aatgcttgcc gtccgccgtc ttcagcgacg aggccagctg cggcggcatg tagttctcgt
     3721 cgtgcttggc cagcacttcg tcggcggtga acaccccgtc gccacccatt gcgccggtgg
     3781 cgacgatgcc ctgcccttcg cggaacaggt ccggcaggat gccgcggtac tgcaccggca
     3841 cgctcttctc gcaatccgac agcgtgaact tgaccagcag gtcggcgccg cgctgcacgc
     3901 tgccgcgctc gaccagcccg cccagacgca gcttggcgcc ggccggtgcg ctgccggtga
     3961 ccaggtcgct cggcgtgtag aaatacatca tgttcttgcg gaaggcggtc acgcccagcg
     4021 cggcggcggc ggacaggccc accagcagcg aggcgaccgc cgccatgcgg cgctggcgtt
     4081 tggtcatggt gcttccacca gcgatttcag cgcgtcgcgc cgctgcgcgc gccaggccag
     4141 cgcattccag ccgtagacga gcgcggccag gccgaagctt ccccagacat agaacgcgta
     4201 gccgcccata tggaagaaac cctcagacat cggcggcgcc cacgaattcc cgcacccagc
     4261 cggcgccgcg ttcgcgcagc agcacttcgt tctgcgtgcg gcgcaggacg gcgtgcgcca
     4321 ccagcagcag cgtgccgagc agcatggcgt acagcggccg cgccatgtcg gcgctgatgt
     4381 tcggctggcc cagcttcagg atggtggaac cctggtgcag gctgttccac cagtccaccg
     4441 agtaatgcac gatcggcacg ttgatcacgc cgatcatcgc cagcaggccg caggcgcgcg
     4501 cgccggcgcg gcggtcgtcg atagcctggt ccaggccgat gacgccgaga tagaggaaca
     4561 gcaggatcag ttccgacgtc agccgcgcgt cccataccca ccacgccccc cacatgggct
     4621 tgccccacag catgccggtg gccagcgcca gcgcggtgaa actcgcgccc accggcgcgg
     4681 cggcgatcag cacgcactcg gccagcttga tgcgccagat caccgcgatg gcgccggcca
     4741 ccgccatcag cgtgtagatc gacagcgaca gcgccgccgc cggcacgtgc acgtagatga
     4801 tgcggaaggc ttcgccctgc tggtaatcgg ccggggccag caccaggccg ccgtagctgc
     4861 cgtaggcgat cagcgccagc gcgctccagc cgagccaggg cgccagcttt tcggcgaagc
     4921 tgaagaagct cggcggcgac gccagcttgt ggaaccagac ccagttcatg ggagagtcat
     4981 tcgtaggaat tgcgcagcgc ggccgtgctt gcccagggaa tcacggtcag cgacagcacg
     5041 aaaatcgcgg cgagaaagta tagcggagcg tccaccggca gaccgctttg cgcggcgcgc
     5101 accgcaccgg cgccgaagat caccaccggc gtgatcagcg gcagcaccag caccggcagc
     5161 agcgcgccgc cgcgcggtaa tcccacggtg agcgcagcgg cgaatccgcc gaccagcgac
     5221 agcaccggcg tgcccagcgc gagcccggcc agcagcaccg gcaggccggc gccgggcaag
     5281 gccagcgcgc ccgcgagcgg cgcggccagc agcaccagcg gcaatccgct gagcagccag
     5341 tgcgcggcga gcttggcgta gacgatggcc agcgcagagt cggccaggaa ccagtgttcc
     5401 agcgtgccgt cttcgaaatc ggcgcggaac agcacctcga gcgacagcag cgccgacagc
     5461 agcgcgccta cccacagcac cgccggcgca aacgcggtca gcagcggatc gttggctttc
     5521 gctcccagcc cgaacagcgt gaccacgatg acgtagaaca gcggtggcat cagccattgc
     5581 agcttgttgc gcgccgcgag cgtgagttcg cgcgcgagcg tggcggcgaa ggcgctcaca
     5641 gcgtcacttc ccgacgcggc agctgggcgg gcaagggctg gtgactggtc accatcgccg
     5701 cgccgccgcc agagaggtgc gccgagagca gctgcgcgat cagcgcgagg ccgtcggcat
     5761 cgaggccatc gagcggttca tccagcagcc atagcggccg cttcgacgcg accagccgcg
     5821 ccagcgcgct acgccgcttc tgcccagccg acagcgtgcg gcaggcgcga tggcgcaggc
     5881 tgccgacgcc cagctgttcg agcgccggct gcaccgcaga tcggtccagc gcgttgatcg
     5941 cgcaccacca gcgcaggttc tccagcgggc tcagcgccag gttgagcgcg ttgcggtggc
     6001 cgatccagtg cagcgcatca ggtcgcgcca cttcgccggc cgccgggcgg cgcaggccgg
     6061 ccagcgtctc cagcaggctg gtcttgcccg cgccgttggc gccgcgcagg tggaccagtg
     6121 cgccgggctc cagcgtgagc gagaaatcct tgagcagcac gcgctcgccg cgggcgacgg
     6181 acaagcgctg tgcggagagg atcggcatgg gccgcgcatt ttacgtagga gcgcgccgtg
     6241 cgcgcgattg gagcgac
//
LOCUS       DASZZC010000646         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_246780,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000646 DASZZC010000000
VERSION     DASZZC010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246780"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VHE37_13770"
     CDS             complement(<1..645)
                     /locus_tag="VHE37_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HVT36652.1"
                     /translation="MLSRTADNLYWLGRYLQRAENTARILNVQALLLLDMPRNTEFGW
                     GVLVDILSAHKLYREHYQDFSEENVVRFLLLDPRNPGSIASSLAAARNILRITRDRLP
                     RELWERVNDLYLRLGESGERALGRARRQEFLTRVTDGALLVYGMLVSNMSHDIGFSFL
                     RLGTNIEQADMTTRIIDVRSADLIRPKTGEDLLPFQNIQWMGVLRSLSAQQMYQR"
     gene            complement(645..>1944)
                     /locus_tag="VHE37_13775"
     CDS             complement(645..>1944)
                     /locus_tag="VHE37_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HVT36653.1"
                     /translation="VMGITFTVYTEGQNVDRAWPFDLVPRVIEKREWQRTEAGLQQRV
                     LALNHFIQDIYGAQKIVKDKVVPAEVLADSVNFRKQCVGMKPKHGVWAHICGTDLVRD
                     ADGTLYVLEDNLRVPSGVSYMVENRQVTKRVFPELFEKTPILPLDDYPEQLYEMLAAM
                     SPRSTAEPVIVLLTPGIYNSAYFEHSWLARQMGVELVEGPDLYVGDDDICYLRTITGP
                     QRVDVIYRRIDDLFLDPEVFREDSALGVKGLMRAWLRGKVALANAPGAGVADDKVIYA
                     FVPQMIRYYLDQDAILPNVPSYLCMDAKQRRHVLANLDALVVKPANESGGYGMLIGPR
                     ASKKEREKFRELIEANPRNYMAQPTLALSTAPTLVDGGVEPRHLDLRPFILSRGGSSY
                     VTTGGLTRVAMVKGSLVVNSSQGGGAKDTWIVDLGDEDGR"
BASE COUNT          321 a          636 c          653 g          334 t
ORIGIN      
        1 gcgctggtac atctgctgcg ccgacagcga gcgcagcacg cccatccact ggatgttctg
       61 gaacggcagc aggtcctcgc cggttttcgg gcggatcagg tcggccgagc gcacgtcgat
      121 gatgcgcgtg gtcatgtcgg cctgttcgat gttcgtgccc aggcgcagga aggagaagcc
      181 gatgtcgtgg ctcatgttcg acaccagcat gccgtagacc agcagcgcgc cgtcggtcac
      241 gcgggtaagg aattcctggc gccgcgcgcg gcccagcgcg cgctcgccgc tttcgcccag
      301 gcgcaggtag agatcgttga cgcgctccca caactcgcgc ggcaggcggt cgcgggtgat
      361 gcgcaggatg ttgcgcgcgg ccgccagcga cgacgcgatc gagccgggat tgcgcgggtc
      421 cagcagcagg aagcgcacca cgttctcctc ggagaaatcc tggtaatgct cgcggtacag
      481 cttatgcgcc gacaaaatgt cgaccagcac gccccagccg aactcggtgt tgcgcggcat
      541 gtcgagcagc agtagcgcct gcacgttgag gatgcgcgcg gtgttctcgg cgcgctgcag
      601 gtagcggccg agccagtaga gattgtcggc ggtgcgcgac agcatcagcg cccatcctcg
      661 tcgccaaggt cgacgatcca agtgtccttc gctccgccgc cctgcgagga gttcaccacc
      721 agcgaacctt tcaccatggc cacgcgcgtg aggccgccgg tggtcacgta ggaactgccg
      781 ccgcgggaca ggatgaacgg acgcaggtcc aggtggcgcg gctcgacgcc gccatcgacc
      841 agcgtcggcg cagtggacag cgccagcgtg ggctgcgcca tgtagttgcg cgggttggcc
      901 tcgatcagct cgcggaattt ctcgcgctct tttttcgatg cgcgcgggcc gatcagcatg
      961 ccgtagccgc ccgactcgtt ggccggcttc accaccagcg cgtcgaggtt ggccagcacg
     1021 tgcctgcgct gcttggcgtc catgcacagg tatgaaggca cgttgggcag gatggcgtcc
     1081 tggtccaggt aatacctgat catctgcggc acgaaggcgt agatcacctt gtcgtcggcc
     1141 acgccggcgc cgggcgcatt ggccagcgcc accttgcccc tgagccaggc gcgcatcagc
     1201 cccttgacgc ccagtgcgga atcctcgcgg aacacctccg ggtcgaggaa caggtcatcg
     1261 atgcggcggt agatgacgtc gacgcgctgc ggcccggtga tggtgcgcag gtagcagatg
     1321 tcgtcgtcgc cgacgtagag gtcggggcct tccaccagct ccacgcccat ctggcgcgcc
     1381 agccaggaat gttcgaaata tgcgctgttg tagatgcccg gcgtgagcag cacgatcacc
     1441 ggctcggcgg tggagcgcgg cgacatcgcc gcgagcattt catagagctg ctcggggtaa
     1501 tcgtccagcg gcaggatcgg cgtcttctcg aacagctcgg gaaacacgcg cttggtcacc
     1561 tgccggttct cgaccatgta ggagacgccc gagggcacgc gcaggttgtc ctcgagcacg
     1621 tacagcgtgc cgtcggcgtc gcgcacgaga tcggtgccgc agatatgcgc ccacacgccg
     1681 tgcttgggct tcatgccgac acactgcttg cggaagttga ccgagtcggc cagcacctcg
     1741 gccggcacca ccttgtcctt gacgatcttc tgcgcgccgt agatgtcctg gatgaaatga
     1801 ttcagcgcca gcacgcgctg ctgcaggccg gcctcggtgc gctgccactc gcgcttctcg
     1861 atcacccgcg gcaccaggtc gaacggccag gcgcggtcca cgttctggcc ctcggtgtac
     1921 acggtgaagg tgatgcccat gacc
//
LOCUS       DASZZC010000647         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1148120,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000647 DASZZC010000000
VERSION     DASZZC010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148120"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VHE37_13780"
     CDS             complement(<1..1160)
                     /locus_tag="VHE37_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36654.1"
                     /translation="MNMRILLGAAALAAMTLAGCGGSGGDSSESALTGAGFSSAGRAN
                     TYTTGEQQTPAVARNAAGNMVVVWDSVGQDGSLSGIYGQRFAGGSAVGAEFQVNTYTR
                     NKQNQPAVSMDAAGNFVVVWRSSGQNRSAGLDGGVIMGQRFAADGTRQGAEFQIGDNM
                     DYDSQADPAVAMNDSGQFVVAYSNRFLLSLAADLELNAYETRYIQARAYNADGSPVGD
                     ATLVYTNVSVQRAPVVGIDQSGNVTVAWLNDSAASAGNNVINPRGIYMQRYSAQMLPS
                     GLPTQVSDSAGASDRPTMAMNAAGQFIIAWERAVDQAELDGIYARIYDASGTATGAQF
                     QVSSAASGFFERASVGIDGSGNYAVVAQSQQGGGSSGVYLQRYAADGSAVGTL"
     gene            complement(1157..2101)
                     /locus_tag="VHE37_13785"
     CDS             complement(1157..2101)
                     /locus_tag="VHE37_13785"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVT36655.1"
                     /translation="MAFADLWRGPGAALALAACVLMSPLAQASFNPWEGVQVIDEDFT
                     ATADGVTRHINYLRPPHVAAGAPAIVMLHYNDGNAQAMANLTDAGELARDFGVWVIMP
                     IAAGGKWGINPNNPRNNDDVGFLSELIDHAVATYGIDAHRVYMTGYSQGGNMAVRFAC
                     EQPLKIAAVSAVASTMLTSLSNQCTPPAPTPMMFVDGTADQQVPYDGTVVGDCVVTNL
                     CSISAPAAAQFWAAADGCPTTPTSSEAVDTVDDGTDIKIDHYGNCTSGARVDLYTVNN
                     GGHTWPGAIDFTPRLGLTSQDIQAGPRMWSYFFSQFSR"
     gene            complement(2311..>3054)
                     /locus_tag="VHE37_13790"
     CDS             complement(2311..>3054)
                     /locus_tag="VHE37_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVT36656.1"
                     /translation="LQDRVLTVRFNRPDKKNALTQAMYSGAAEAFADAKSNAQVRVVL
                     IAGAPDCFCAGNDIVDFLNSPPGDENSPVARFMHTLSALDKPVIAAASGLAIGIGVTL
                     LLHCDLVFLGEKTRLEMPFVNLGICPEFASSYLLPRVMGYQRAGELLLCGAFDAQKAR
                     DYGIANFIAPNAEVEAQARARALSIAQLPPNAVRVTKGLLRRWSETTAKEAMAAEVQN
                     FMPMLRQPEALEALNAFTQKRKPDFSRFS"
BASE COUNT          512 a          982 c         1026 g          534 t
ORIGIN      
        1 agcgtgccca ccgcgctgcc atcagcagca tagcgctgca ggtaaacacc gctggaaccg
       61 ccgccctgct gcgactgcgc caccaccgcg tagttgccgc tgccgtcgat gccgacggac
      121 gcgcgttcga agaaacccga cgcagcactg ctcacctgga actgcgcacc ggtggccgtg
      181 ccgctggcgt cgtagatgcg cgcatagatg ccatccagct ctgcctggtc caccgcgcgc
      241 tcccaggcaa tgatgaattg cccggccgcg ttcattgcca tggtcggacg atccgacgcg
      301 cccgcggaat cgctcacttg cgtgggcagg cccgagggca gcatctgcgc gctgtagcgt
      361 tgcatgtaga tgccacgcgg gttgatcacg ttgttgccgg cgctggcggc gctgtcgttg
      421 agccaggcca cggtgacgtt gcccgactgg tcgatgccca ccaccggcgc gcgctgcacg
      481 ctgacattgg tgtagaccag cgtggcgtca ccgaccgggg agccgtccgc gttgtaggcg
      541 cgcgcctgga tgtagcgcgt ctcgtaagcg ttgagttcga ggtcggcggc cagcgacagc
      601 aggaagcggt tggagtaggc gacaacaaac tggccgctgt cgttcatcgc cactgccggg
      661 tcggcctgcg agtcgtagtc catgttgtcg ccgatctgga attccgcccc ctgccgcgtg
      721 ccgtcggcgg caaaacgctg gcccatgatg acgccgccgt cgaggccggc gctgcggttc
      781 tggcccgagc tgcgccacac cacgacgaaa ttgccggcgg catccatcga cactgccggc
      841 tggttctgct tgttgcgggt gtaggtgttg acctggaatt ccgcacccac cgcgctgccg
      901 ccggcgaagc gctgtccgta gatgccggac aggctgccgt cctggcccac gctgtcccac
      961 accaccacca tgttgccggc ggcgttgcgc gcaacggcgg gtgtctgttg ctcgccggtg
     1021 gtataggtgt tggcgcggcc ggcggacgaa aagcccgcgc cggtcaacgc gctttcagat
     1081 gagtcgccgc cgctgccgcc gcatccggcc agcgtcatcg cggccagcgc cgcggctcca
     1141 aggagtattc gcatgttcat cggctgaact ggctgaagaa ataggaccac atgcgcgggc
     1201 cggcctggat gtcctgcgaa gtcagcccga gccgcggcgt gaagtcgatg gcaccgggcc
     1261 aggtatggcc gccgttgttc acggtgtaca ggtccacccg cgcgccgctg gtgcagttgc
     1321 cgtagtgatc gatcttgatg tcggtgccgt catcgaccgt gtccaccgct tcgctcgaag
     1381 tcggcgtcgt gggacagcca tcggcggcgg cccagaattg cgcggcggct ggcgctgaaa
     1441 tcgagcagag gttggtgacg acgcaatcgc ccaccacggt gccgtcatag gggacctgct
     1501 ggtcggcagt gccgtcgacg aacatcatcg gcgtgggcgc gggcggcgtg cactggttgg
     1561 acagggaggt gagcatggtc gacgccacgg ccgacacggc ggcaattttc aacggctgct
     1621 cgcaggcgaa acgcaccgcc atgttgccgc cctgcgaata gccggtcatg tacacgcggt
     1681 gcgcgtcgat gccgtaggtg gccaccgcat ggtcgatcag ttcggagagg aagccgacgt
     1741 catcattgtt gcgcgggttg ttggggttga tgccccactt gccgcccgcc gcgatcggca
     1801 tgatcaccca cacgccgaag tcgcgcgcca gttcgccggc gtctgtcagg ttggccatgg
     1861 cctgcgcgtt gccgtcgttg taatgcaaca tgacgatggc cggcgcaccg gccgccacgt
     1921 gcggcggccg caggtaattg atgtggcgcg tcacgccgtc cgcggtggcg gtgaagtcct
     1981 cgtcgatcac ctgcacccct tcccagggat tgaacgaagc ctgcgccagc ggcgacatca
     2041 gcacgcatgc tgccagcgcg agcgcggcgc cgggcccgcg ccagagatct gcaaaagcca
     2101 tcagggaatt tccgtcagag agaagtgccg ccccacgcgg cgcgcctagg ataagggtgc
     2161 ggcgcgcgcc acgtgagggc gattggcaaa aaaatctatc ggtaaaggcg cctttacagt
     2221 tcaccgcatg gcgcatttct atgttattaa atgatgtata tagttaacta taaataataa
     2281 ataataacat caccggacgg ggtgcggtga tcagctgaag cgcgaaaaat ccggcttgcg
     2341 cttctgcgtg aaggcattga gcgcctccag cgcttcgggc tggcgcagca tcggcatgaa
     2401 gttctgcacc tccgccgcca ttgcttcctt cgccgtggtt tccgaccagc gccgcagcag
     2461 gcccttggtc acgcgcaccg cgttgggcgg cagctgcgcg atggacagcg cacgtgcacg
     2521 cgcctgcgcc tcgacctccg cgttgggcgc gatgaagttg gcgatgccgt agtcgcgcgc
     2581 cttctgcgca tcgaacgcac cgcacagcag gagttcgccg gcgcgctggt agcccatgac
     2641 acgaggcagc aggtagctcg atgcgaattc cgggcagatg ccgagattga cgaagggcat
     2701 ctccaggcgc gtcttctcgc cgaggaacac caggtcacag tgcagcagca gggtgacgcc
     2761 gatgccgatg gccagcccgc tggccgcggc gatcaccggc ttgtccagcg ccgacagcgt
     2821 gtgcatgaag cgcgcgaccg gcgagttctc gtcgcccggc ggcgagttga ggaaatcgac
     2881 gatgtcattg ccggcgcaga agcagtccgg cgcgccggcg atcagcacca cgcgcacctg
     2941 ggcattggac ttcgcatcgg cgaacgcttc cgcggcgccg gagtacatcg cctgggtcag
     3001 cgcgttcttc ttgtccgggc ggttgaagcg cacggtgagt acgcggtctt gcag
//
LOCUS       DASZZC010000648         6296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1148255,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000648 DASZZC010000000
VERSION     DASZZC010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148255"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VHE37_13795"
     CDS             complement(<1..589)
                     /locus_tag="VHE37_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36657.1"
                     /translation="MKLKTMLIVAALGLASWGAQAQNGTPQREIMDDRWNVWLMGGGI
                     FSDSSNLDKGYTGALHFTHPLADHFAFNGGLDYGSLGTSNAGKYNRISARLGVELFPW
                     SPYYDSSSSLQPFIGAGGHASSVSFIGEHRAAYGPYADFGLQQRLGQSLWFRLQGSYQ
                     QDYVQAKGIFPRDNFYTWQALAAVGFSLGEKPYDHN"
     gene            complement(624..2627)
                     /locus_tag="VHE37_13800"
     CDS             complement(624..2627)
                     /locus_tag="VHE37_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVT36658.1"
                     /translation="MLTRLIHAASIAALALLAACSARSPDLPVPKLVSGPVVSCVPQG
                     PISTGQTSQCTATQCVFETIDASGNANQVPGQCPAVTWSTSPTSVGTIDGNGLLTGVG
                     AGIVTAVATVEGGSGSTTVAVNAACVGSIAITPTNATLIAGQNLQYTATATFSDNHQA
                     NVTQQTTWTSGDTNTATIDNKGLAHGVAASATPVTITGSYAGTNICSGTASPIVQATT
                     VTVSAAQLIANGLCIEPAADAASQFTGCRADTGACASTATPVNLTIGQTAPFRLRGRF
                     NNGQECNLTNDPKTTWGSSAPTVATVSSAGLATGVAAGTGSATATYTDATANPANVTA
                     APYPLKVSVSQVLTKDSLRVSAKPYTVTDTPRKFACVGATDIVGSLEDSSQIQGRLKV
                     FAGARFCATNQVNADGTCKQFTGNAISDVTNDDGVTVTTPNANRIVWSQSSSYWNGNA
                     CVSTLQGALDSIAKGPTGVVGDTYTPAARYATGDRQPGENGVVVGTGDVRVGFACITA
                     EYTNPQLATNKASDGMTVLVLPVTNDVLLGPSSANDATHLCDALEPLFQMTASDNGGN
                     GALTQLLSAVTEIVNPIAQNVADGSEPGGPLPVTTIVTQVLTALSDNATSQVFAAGLT
                     NAVNTVDTGVYSPVVCALGSLLNAITTQDPASAANAQACLPTP"
     gene            2836..3756
                     /locus_tag="VHE37_13805"
     CDS             2836..3756
                     /locus_tag="VHE37_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVT36659.1"
                     /translation="MTQLRIGIDLGGTKIEGIVMAPGSRIVARQRIATPKDDYTGIIA
                     AIAQLVQQLERQVQAGPLPVGMGHPGAISPATGLLKNSNSTCLNARPLQRDLQQALGR
                     EVRMANDADCLAVSEAHDGAAAGADNVFAVILGTGVGGGIAINRGLLRGANAIAGEWG
                     HNPLPWARPEWDEVPGPRCWCGRHGCIETWLSGTGLAADHLRVTGEKIGGEQIVQRAQ
                     AGDAACTATLARFEDRLARALAHIINILDPQVIVLGGGVSQVQRLYQHVPALWDQWVF
                     SDRVDTQLRPPQHGDASGVRGAAWLWPEDA"
     gene            3811..4176
                     /locus_tag="VHE37_13810"
     CDS             3811..4176
                     /locus_tag="VHE37_13810"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HVT36660.1"
                     /translation="MNWYLEVLQKKYAQFDGRAHREEFWMFVLVNFLASLAVGIVAWI
                     IHLQLLRALYALAVLVPSLALGARRLHDTHRSGWWQLIVLIPVIGWIVLLVFFVQPGD
                     AGDNSYGADPRGAAPAAAS"
     gene            complement(4303..5196)
                     /locus_tag="VHE37_13815"
     CDS             complement(4303..5196)
                     /locus_tag="VHE37_13815"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HVT36661.1"
                     /translation="MRKLICLLLLGGAVPAQAAAPSYAHVEALQPPAWVLRGDARTAL
                     APGMPLYAGERYLTGEHARVHFALDDGSVVKLGEQAEFSLPRLSLKKGQDQTAVLQGA
                     LQLLKGAFRFTTSVLGHLKRRELDIAVGPTITAGIRGTDIWGESDSEQELLCLIEGKL
                     DVRSPGQSAVTMDQPKTFYVVPRGQGPQPVVATPPEKLEGWIPQTELIGQEVSLHARA
                     RWSLVLAQYLEVARARERVAQLDAAGYPVSIVQRPSKDIVLQALVTEGFRSRDDAQRY
                     AAELQQKFDFVQKPQVLAPAN"
     gene            complement(5266..5814)
                     /gene="ppa"
                     /locus_tag="VHE37_13820"
     CDS             complement(5266..5814)
                     /gene="ppa"
                     /locus_tag="VHE37_13820"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HVT36662.1"
                     /translation="MSLQLVPAGRDVPNDINVVIEIPMNSEPVKYEVDKASGAIFVDR
                     LLTTPMRYPCNYGYVPHTLGGDGDPLDALVMMPMPLLPGSVIRCRPIGMLQMTDEGGS
                     DEKLVVVPHSKVSPLYNDVETVRALPALIRDQIAHFFAHYKDLEKGKWVKIEGWHGPN
                     DARKVISEGVQRFDDSPKKPNF"
     gene            5962..>6296
                     /locus_tag="VHE37_13825"
     CDS             5962..>6296
                     /locus_tag="VHE37_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT36663.1"
                     /translation="MSSLQKILVVVDRRLRRSPAVDRALGLARKSGAQLHLCLFDHDA
                     LIEKTADRVSPEVMHLAKAQYLQQRRDWLISLAASLADSGVPVECVVVWSPVIDEAIL
                     DKILDVEPDL"
BASE COUNT          968 a         2032 c         2176 g         1120 t
ORIGIN      
        1 ggttatggtc atagggcttt tcgcccagcg agaaaccgac cgctgccagc gcctgccagg
       61 tgtagaagtt gtcgcgcggg aaaatgccct tggcctgcac gtagtcctgc tggtagctgc
      121 cctgcagccg gaaccacagg ctctggccca ggcgctgctg caggccgaag tctgcgtacg
      181 ggccgtaagc cgcgcgatgc tcgccgatga agctcacgct cgacgcatgg ccgcccgcgc
      241 cgatgaaggg ctgcagcgat gacgacgagt cgtagtacgg gctccacgga aacagttcca
      301 cgcccaggcg cgcgctgatc cggttgtact tgccggcgtt gctggtgccc agcgagccat
      361 agtccaagcc gccgttgaag gcgaagtgat ccgccagcgg atgggtgaag tgcagcgctc
      421 cggtataacc cttgtcgagg ttggagctgt ccgaaaagat gccaccgccc atcagccaca
      481 cgttccagcg gtcgtccatg atctcgcgct gcggcgtgcc gttctgcgcc tgcgcgcccc
      541 aggaggcgag ccccagggcc gccactatca acatcgtctt cagcttcatt gttgttctgg
      601 tccgtccttg tgatcagtga ggttcagggc gtgggcaaac acgcctgtgc attggccgcc
      661 gacgccgggt cctgcgtggt gatggcgtta agcagcgagc cgagcgcaca caccaccggt
      721 gagtaaacgc cggtatccac cgtgttcacc gcgttggtca ggccggcggc aaacacctgc
      781 gaggtcgcat tgtccgacaa ggcggtgagc acctgggtga cgatggtggt caccggcagc
      841 gggccgcccg gctcgctgcc gtcggccacg ttctgcgcga tggggttgac gatctcggtg
      901 accgccgaca gcagctgcgt cagcgcgcca ttgccgccgt tgtcggacgc ggtcatctgg
      961 aacaagggtt ccagcgcgtc gcacaggtga gtggcgtcgt tggcgctgga gggaccgagc
     1021 agcacgtcgt tggtcaccgg caacaccaac acggtcatgc cgtcgctggc cttgttggtg
     1081 gccagctgcg ggttggtgta ttccgcggtg atgcaggcga aacccacgcg cacatcgccg
     1141 gtgccgacca cgacaccgtt ctcgccgggc tggcggtcac cagtggcata gcgcgcagcc
     1201 ggggtgtagg tgtcgccgac tacgcctgtc ggccccttgg cgatgctgtc gagtgctccc
     1261 tgcagcgtcg acacgcaggc gttgccgttc cagtaggacg agctctggct ccagacgatg
     1321 cggttggcgt taggcgtggt cacggtgaca ccgtcatcgt tggtcacgtc gctgatggcg
     1381 ttgccggtga actgcttgca cgtgccatcg gcattcacct ggttggtggc gcagaagcgc
     1441 gcaccggcga acactttgag ccgcccctgg atctggctgc tgtcttccag cgagccgacg
     1501 atgtcggtgg cgcccacgca ggcgaacttg cgcggcgtgt cggtgaccgt gtacggcttg
     1561 gccgacacgc gcaggctgtc cttggtcagc acctggctca cgctgacctt cagcggatac
     1621 ggcgcggccg tcacgttggc cggattggcg gtggcgtccg tgtaggtggc ggtggcactg
     1681 ccggtgcccg ccgcgacgcc ggtggcgaga cccgcgctcg acaccgtggc aaccgtgggc
     1741 gccgacgaac cccaggtggt cttcgggtcg ttggtgaggt tgcactcctg gccgttgttg
     1801 aagcggccgc gtagccggaa cggcgcggtc tggccgatgg tgaggttgac cggcgtggca
     1861 gtcgacgcgc aggcaccggt gtcggcacgg cagccggtga actgcgaggc cgcgtccgcc
     1921 gccggctcga tgcacaggcc gttggcgatc agctgcgcgg cactcacggt gaccgtggtg
     1981 gcctggacga tcggcgaggc ggtgccgctg cagatgttgg tgccggcata gctgccggtg
     2041 atggtcaccg gcgtggcgct ggcggcgacg ccgtgggcca ggcccttgtt gtcgatggtg
     2101 gccgtgttgg tgtcgccgct ggtccaggtg gtctgctgcg tcacgttggc ctggtggttg
     2161 tcgctgaagg tcgcggtggc tgtgtactgc aggttctggc cggcgatcag cgtggcattg
     2221 gtcggcgtga tggcgatgct gcccacgcag gcggcgttga ccgccaccgt ggtgctgccg
     2281 gagccgcctt ccaccgtggc gacggccgtc acgatgccgg cgccgacgcc agtgagcagg
     2341 ccgttgccgt cgatggtgcc gaccgaggtc ggcgatgtgg accaagtgac cgccgggcac
     2401 tggccaggta cctggttggc gttgccgctg gcgtcgatcg tttcgaacac gcattgcgtc
     2461 gccgtgcatt gcgaggtctg acccgtggag atgggtccct gcggcacgca gctcaccacc
     2521 ggtccgctga ccagcttggg tacggggaga tcggggctgc gggcgctgca ggcagcaagc
     2581 agggcgagcg ccgcgatgga cgcggcgtgt atcaggcggg tcagcatgga cattctcatc
     2641 gatgaaacgc ggcagaattg ctccgcggcg tacgtcctct gtacggacgc ggtaggttaa
     2701 tgaatagttt gcgcgaagaa cggtgacaag ttccatcgcg cggcgaggtt ggggcgtagg
     2761 ccgctgcacc aaccctgcgt aaggccattc ttacatggaa caattgctgt gctgtacctc
     2821 tcactggctt aagacatgac ccagttgcgc atcggcatcg acctcggcgg caccaagatc
     2881 gaaggcatcg tcatggcgcc cggctcgcgc atcgtcgcgc gccagcgcat cgccacgccc
     2941 aaggacgatt acacgggcat catcgcggcc atcgcgcagc tggtgcaaca actcgaaagg
     3001 caggtgcagg cggggccgct gccggtgggc atgggtcatc cgggcgccat ttcgccggcc
     3061 accggcctgc tgaagaattc gaattccacg tgcctcaacg cgcgcccgct gcagcgtgac
     3121 ctgcagcagg cgctgggccg cgaagtgcgc atggccaacg atgccgattg cctggcggtg
     3181 tccgaggcgc acgacggcgc ggcagccggc gccgacaacg tgttcgccgt gatcctgggc
     3241 accggcgtcg gcggcggcat cgccatcaac cgcggtctgc tgcgcggcgc caatgccatc
     3301 gccggcgagt gggggcacaa cccgctgccg tgggcacgcc cggaatggga cgaagtgccc
     3361 gggccgcgct gctggtgcgg ccgccatggc tgcatcgaga cctggctgtc cggcaccggg
     3421 ctggcggccg accatctgcg cgtcaccggc gagaagatcg gcggcgagca gatcgtgcag
     3481 cgcgcgcagg ctggcgatgc cgcgtgcacc gccacgctgg cgcgtttcga ggaccggctg
     3541 gcgcgcgcac tggcgcacat catcaacatc ctcgatccgc aggtgatcgt gctcggcggc
     3601 ggcgtgtcgc aggtgcagcg gctgtaccag cacgtgccgg cgctgtggga ccagtgggtg
     3661 ttttccgatc gcgtcgacac gcagctcagg ccgccgcagc atggcgacgc cagcggcgtg
     3721 cgcggcgcgg cctggctgtg gcccgaggac gcctgagcgc actacactcc ggccacttcc
     3781 acgcaacaac agcctaaggg gtcggacacg atgaactggt atctcgaagt cctgcagaag
     3841 aaatacgcgc agttcgacgg gcgcgcgcat cgcgaggaat tctggatgtt cgtgctggtg
     3901 aatttcctgg cgagcctggc ggtgggcatc gtcgcctgga tcatccacct gcagttgctg
     3961 cgcgcgctct acgcgctggc cgtgctggtg ccgagcctgg cgctgggcgc gcgccgcctg
     4021 cacgacacgc atcgcagcgg ctggtggcag ctgatcgtgc tgattcccgt gatcggctgg
     4081 atcgtgctgc tggtgttctt cgtccagccc ggcgatgccg gcgacaacag ctatggcgcc
     4141 gatccgcgcg gtgcggctcc ggcggccgca agctgaagcg gcaggcaaaa aacctcgtta
     4201 ccgcactgcc tgtttcgctg ttataaaaaa ttatattatt ttaatttaat acaataataa
     4261 ataacacgcg gaatagcggt gcggtgcagc tgtttttgcc gctcagttgg ccggcgccag
     4321 cacctgcggc ttctgcacga agtcgaattt ctgctgcagc tccgcggcgt agcgctgcgc
     4381 gtcgtcgcgg ctgcggaaac cttcggtgac cagcgcctgc agcacgatat ccttcgacgg
     4441 ccgctgcacg atgctcaccg ggtagccggc cgcatcgagc tgcgccacgc gctcgcgcgc
     4501 ccgcgccact tccaggtatt gcgccagcac caggctccag cgcgcgcgtg cgtgcagcga
     4561 aacctcctgc ccgatcagct cggtctgcgg gatccagccc tcgagttttt ccggcggcgt
     4621 cgccaccacc ggctgtgggc cctggccgcg cggcaccacg tagaaggttt tcggctgatc
     4681 catggtcacg gccgactggc cggggctgcg cacgtcgagc ttgccttcga tcaggcacag
     4741 cagctcctgc tcgctgtcgg actcgcccca gatgtcggtg ccgcggatgc cggcggtgat
     4801 ggtggggccc accgcaatgt cgagctcgcg ccgtttcaga tggcccagca cgctggtggt
     4861 gaaacggaac gcgcccttga gcaactgcag cgcaccctgc agcacggcgg tctggtcctg
     4921 gcccttcttc agcgacagcc tgggcagcga gaactccgcc tgctcgccca gcttgaccac
     4981 gctgccgtca tcgagcgcga agtgcacgcg cgcgtgctcg ccggtgaggt aacgctcgcc
     5041 ggcatacagc ggcatgcccg gggccagcgc cgtgcgcgcg tcgccgcgca gcacccaggc
     5101 tggcggctgc agtgcctcga catgcgcata ggacggagcc gcagcctgcg ccggaacggc
     5161 gccgccaagc agcagcaggc agatcagttt gcgcatcgcg cagttctccc caacagtctt
     5221 gcgtcctgat aacggcgttg cgcctcttgc agatgcgcgg ccgctctaga aattcggttt
     5281 cttgggtgag tcgtcgaagc gctgcacccc ctcgctgatc accttgcgtg cgtcgttggg
     5341 gccgtgccag ccctcgatct tcacccactt gcccttctcc agatccttgt agtgcgcaaa
     5401 gaaatgcgcg atctggtcgc ggatcagcgc gggcagcgcg cgcaccgtct caacgtcgtt
     5461 gtacagcggg ctgaccttgc tgtgcggcac caccaccagc ttctcgtcgc tgccgccttc
     5521 gtcggtcatc tgcagcatgc cgatcggacg gcagcggatc acgctgcctg gcagcagcgg
     5581 catcggcatc atcaccagcg cgtcgagcgg atcgccgtcg ccgccgagcg tgtgcggcac
     5641 atagccgtaa ttgcacgggt agcgcatcgg tgtggtcagc aggcggtcga cgaagatggc
     5701 gccggacgcc ttgtccacct cgtacttgac cggctcgctg ttcatcggga tttcgatgac
     5761 cacgttgatg tcgttgggga cgtcgcgccc tgccgggacc agttgcagtg acatgggaaa
     5821 cagccctggg gaaattttgg ggcgcggatt atatagccag ccgcgtgcgc ggagccgtgc
     5881 gcccgtccgc gcgttgaccc acgtcaatgc cccgcgccag gggcggtgct gcaatgccgc
     5941 cgtacacagc acgaggcagt catgagttcg ctgcagaaaa tcctggtggt ggtcgaccgg
     6001 cgcctgcgcc gcagcccggc ggtggaccgc gcgctcggcc tggcgcgcaa gagcggcgcg
     6061 cagctgcacc tgtgcctgtt cgaccacgac gcgctgatcg agaagaccgc cgatcgcgtg
     6121 tcacccgaag tcatgcacct ggccaaggct cagtacctgc agcagcggcg cgactggctg
     6181 atttcgctgg ccgcctcgct ggccgacagc ggcgtgccgg tggagtgcgt cgtggtgtgg
     6241 tcgccggtga tcgacgaggc gatcctggac aagattctcg atgtggaacc cgacct
//
LOCUS       DASZZC010000649         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_688820,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000649 DASZZC010000000
VERSION     DASZZC010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688820"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VHE37_13830"
     CDS             complement(<1..296)
                     /locus_tag="VHE37_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT36664.1"
                     /translation="MTSSCAIRQLPSLDDAAVEQLAGVLIDCVEGGASVSFMLPLTRE
                     RARAFWRKVSAGVAAGERAVLVAEDALGICGTVQLIYDLPENQPHRADLAKMLV"
     gene            complement(344..772)
                     /locus_tag="VHE37_13835"
     CDS             complement(344..772)
                     /locus_tag="VHE37_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36665.1"
                     /translation="MPSDDASGARLPAVDCAQLMDEIAVSLRPRALERRLQLEVICPP
                     SCMLAIDAAVLRQLLLELLGVAIDLAHAGSVIFVLRPGVGGITDFVISNTTAGGEPPV
                     QQALQTTLQRARSMAAAIGGRIVHKHDAGGASIFMLILGA"
     gene            1160..1441
                     /locus_tag="VHE37_13840"
     CDS             1160..1441
                     /locus_tag="VHE37_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT36666.1"
                     /translation="MKMNNKEPARRSSRRFLAHNLMIARTIHGWSQEQLGLQSGLKRT
                     YIGAIERCEINPGVDNLDKLAKAIGVASHVLILEPEQAHALLYRSRNGN"
     gene            1490..>2984
                     /locus_tag="VHE37_13845"
     CDS             1490..>2984
                     /locus_tag="VHE37_13845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HVT36667.1"
                     /translation="MHPLSATESLLDQLPVGAFRLDGGLREMYVNRLMAEFTGLPHGR
                     IDTARLASLPLPGDLRKKWLAASQAALRDGATQIIEFTYPLGDAIKQVSLRIAPERGA
                     GGAISGVLGTAMDISQLRRTEDALRESERRFEAFMDHTPAIAWMKDPQGRYIYRNKAF
                     RDRYGEPGEDWSGRSDDDFFAPEDAAMFASTDLSVIATGKPVQFETSSSDRQGRSSDW
                     LIQKFPLRDSSGRFFVGGVGVLIDERKRLEQALAESESRFQAFLDHSPTLSWMKDEDG
                     RYLYVSETYKQFLGIDDDRWRGKTDFDLFDRHFAQRCRELELKVLADGISLETDGPAP
                     DARGGEHHWLLVRFLFRDIAGGRFVGGVATDVTRRQRAEELVRLQSLTDEMTGLYNRR
                     GFKLLVEQELRHVRRQREHCALLYVDLDGLKGINSAHGHEGGDSAIVTVAEALRVAVR
                     NSDIVARVGGDEFVVILVDTDADQAQAIADRLRESGAHSAPVPFSLGW"
BASE COUNT          516 a         1031 c         1003 g          434 t
ORIGIN      
        1 tgcaccagca tcttggccag gtcggcgcgg tgcggctggt tttccggcag gtcatagatc
       61 agctgcaccg tgccgcagat gcccagcgcg tcttccgcca ccagcacggc gcgctcgccg
      121 gccgccacgc ccgcggagac cttgcgccag aacgcccgcg cgcgctcgcg cgtgagcggc
      181 agcatgaagc tgaccgaggc gccgccctcg acgcagtcga tcagcacgcc ggccagttgc
      241 tcgacggccg cgtcatcgag tgacggcagc tgtcgtatcg cgcaggaaga tgtcatggcg
      301 ccacagcgtt tcgcacgccg cgcgcgccgt caaccgctgc gggtcacgcg cccaggatca
      361 gcatgaagat gctggcgccg ccggcatcgt gtttgtgcac gatgcggccg ccgatggcag
      421 cggccatgct gcgcgcacgc tgcagcgtgg tctgcagcgc ctgttgcacc ggcggttcgc
      481 cgcccgcggt ggtgttgctg atgacgaaat cggtgatgcc gccgactcct ggtcgcagca
      541 cgaagatgac cgagccggcg tgcgcaagat cgatggcaac gccgagcagt tccagcagca
      601 gctggcgcag gacagccgcg tcgatggcga gcatgcagga cggagggcag atcacttcca
      661 gttgcagacg gcgttcgagc gcgcgcgggc gcaggctgac ggcaatctcg tccatcagct
      721 gcgcgcagtc gaccgcaggc aacctggcgc cgctggcatc gtcggagggc atcaggaccc
      781 ccggcgggaa attgcgctga cggcggcagc gcgcgcagta gaaacttcag gcatggataa
      841 catggctatt tccattaaac gatgccttct tgccatgaat acatggcgta gctgggctcc
      901 atgaaactcg gcctgtgcgg gcaaggcaag actcgtccga ccagcggcac gcgccggcag
      961 tccggcaatg acctgagtca gtgccgccac ccggcgagcc atgcaagctg ctccgcatgc
     1021 gaccgccggc tcctgccaag ttgctcaccc gccttattgg gcagcaaagc gcccccaata
     1081 tggggcgatg cgcctgtttg aaggggtcgc agtcgcgctg gcgctgccgc agcatgtggc
     1141 gcgttgggga acccgtctca tgaagatgaa caataaagag ccggcgcggc gctcgtcgcg
     1201 gcgttttctt gcacacaacc tgatgatcgc ccgcaccatc cacggctggt cgcaggaaca
     1261 gctcggcctg cagagcgggc tcaagcgaac ctacatcggc gccatcgagc gctgcgagat
     1321 caaccccggc gtcgacaatc tcgacaagct ggccaaggcc atcggcgtcg ccagccacgt
     1381 gctgatcctg gaacccgagc aggcgcacgc actgctgtac cgctcgcgca acggcaactg
     1441 accgcccgcg gcgcgcggct cggctaagat ggcgcccgca gaaagcgcca tgcacccact
     1501 cagcgcaacg gagtcgctgc tggaccaact gccggtcggc gccttccggc tggacggcgg
     1561 tctgcgcgag atgtacgtca accgcctgat ggcggaattc accggcctgc cgcacgggcg
     1621 catcgatacc gcgcgcctgg cttcgctgcc gctgcccggc gacctgcgca agaaatggct
     1681 ggccgcgtcg caggccgcgc tgcgcgacgg cgccacccag atcatcgagt tcacctatcc
     1741 gctgggcgac gcgatcaagc aggtcagcct gcgcatcgca cccgaacgcg gcgccggcgg
     1801 tgccatcagc ggcgtgctcg gcaccgccat ggacatcagc cagctgcgcc gcaccgagga
     1861 cgcattgcgc gaaagcgagc ggcgcttcga agccttcatg gaccacacgc cggccatcgc
     1921 ctggatgaag gacccgcagg gccgctacat ctaccgcaac aaggcgttcc gcgaccgtta
     1981 cggcgagccg ggcgaggact ggagcgggcg cagcgacgac gatttcttcg cgccggagga
     2041 tgcggcgatg ttcgccagca ccgacctgtc ggtcatcgcc accggcaagc cggtgcagtt
     2101 cgagaccagc agcagcgacc gccagggccg cagctcggac tggctgatcc agaaattccc
     2161 gctgcgcgat tcctccgggc gcttcttcgt cggcggggtc ggcgtgctga tcgacgaacg
     2221 caagcgcctg gagcaggcgc tggccgagag cgagtcgcgc ttccaggcgt tcctcgacca
     2281 cagcccgacg ctgtcgtgga tgaaggacga ggacggccgc tacctctacg tcagcgaaac
     2341 ctacaagcag ttcctcggca tcgacgacga ccgctggcgc ggcaagaccg atttcgacct
     2401 gttcgaccgc catttcgcgc agcgctgccg cgagctggaa ctcaaggtgc tggccgacgg
     2461 catctcgctg gagaccgatg gcccggcacc cgacgcgcgg ggcggcgagc accactggct
     2521 gctggtgcgc ttcctgttcc gcgacatcgc cggcggccgc ttcgtcggcg gcgtggccac
     2581 cgacgtcacc cgccggcagc gcgcagaaga actggtacgc ctgcaatcgc tgaccgacga
     2641 gatgaccggc ctgtacaacc gccgcggctt caagctgctg gtggaacagg aactgcgcca
     2701 cgtgcggcgc cagcgcgagc attgcgcgct gctctacgtc gacctcgacg ggctcaaggg
     2761 catcaacagc gcgcacggcc acgaaggcgg cgacagcgcc atcgtcaccg tggccgaggc
     2821 cctgcgcgtg gcggtgcgca acagcgacat cgtggcgcgc gtgggcggcg acgagttcgt
     2881 ggtgattctg gtggacaccg acgccgacca ggcgcaggcc atcgccgacc ggctccgcga
     2941 gtcgggcgcg cattcggccc cggtgccctt ttcgctgggg tggg
//
LOCUS       DASZZC010000650         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_875672,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000650 DASZZC010000000
VERSION     DASZZC010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875672"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VHE37_13850"
     CDS             complement(<1..260)
                     /locus_tag="VHE37_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVT36668.1"
                     /translation="MKYLLDSNCIAAAVKGRLPMAIRLSSLKPCDVAISAIGRMEVEA
                     GLRGNPRATTRYGKLLHEFLDAVRVLDFGASEAQQAVTVGNYL"
     gene            complement(257..508)
                     /locus_tag="VHE37_13855"
     CDS             complement(257..508)
                     /locus_tag="VHE37_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVT36669.1"
                     /translation="MNFSVHFDEATLDRLAAAVGRTGLTRNRIIVLAVQQWLDRNEEP
                     DWPRTLKEHFQNPAPELAANDDADLVLQRPDAAAAGVRW"
     gene            complement(605..3274)
                     /gene="gyrA"
                     /locus_tag="VHE37_13860"
     CDS             complement(605..3274)
                     /gene="gyrA"
                     /locus_tag="VHE37_13860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVT36670.1"
                     /translation="MTEFAKEILTVNLEDEMRRSYLDYAMSVIVGRALPDARVGLKPV
                     HRRSLYAMAEQKNDYNKPFKKSARIVGDFMGKYHPHGDSAIYDTIVRMAQPFSLRYLL
                     VDGQGNFGSVDGDNPAAMRYTEIRMTRIAHELMADIDKETVEFQPNYDGSEQEPTVLP
                     ARFPNLLVNGSSGIAVGMATNIPPHNLSEVIDGCVALIDNPAIDLASLMKLVPAPDFP
                     TGGLLLDTHGLVGAYATGRGRVVIRARTEFEPIGNDKQAIIVTELPYQVNKARLLEKI
                     AELVKDKRIEGISELRDESDKDGMRMVIELKRGENAEVVLNNLFQQTQLQVVFGINMV
                     AIDNGQPRTLSLKQLLEIFVRHRRDVVTRRTRYLLREARKRAHVLEGLSIALANIDEI
                     IELIRRSPNPAEAKAGLVARLWQPGGVTAMLERSGAAASRPEDLPDELGLVEGGYRLS
                     EAQAQAILDLRLHRLTGLEQDKIHDEFKELLEAIADYLDILGSEARLLSVIRAELLEV
                     KEQFGDERRSEITQSALDIAHEDLIAPQDMVVTLSHEGYVKTVPLIEYQAQKRGGRGK
                     LAAATKEEDFVDRLWIAHTHDTLLCFSSAGKCYKLRVFELPTGSRAGRGKPFNNLLPL
                     EGAEKINAVLPIKSFDDPVYAGKYIFMATRRGTVKKTELAAFANVRANGIIAVDLRVD
                     DTLVDVALTSGSDDVILISDAGRAIRFNEGAVRVMGRGATGVRGMRLIKSFAAGEEEA
                     EEAGAEAPVDEAEEVIENGNGGSDGARLIALIVPTSGDILTVSEFGYGKRTPVEDFPR
                     KGRGGQGVIGQALTDKTGRLVGAVVVDDSHEIMLISEAGNLIRTRAKEVRVAGRNTQG
                     VRIMRPVEGDRLVGLDRVEADESAEESPGGSAE"
     gene            3550..4047
                     /locus_tag="VHE37_13865"
     CDS             3550..4047
                     /locus_tag="VHE37_13865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVT36671.1"
                     /translation="MKRLAALALLACFGCAHAAAPEVGSTAPPFSAQLLDGTRFSLPA
                     GHDRVVLINFWATWCEPCQAELAAFAAYMRAHPQAPLTIIAVSMDDPERRAQVAQVAA
                     KLPFAVAIADNVKAERYGRIWRLPMSFVVDRAGVLRVDGGRGEQVPFDLPTLERTITP
                     LLQNE"
     gene            complement(4035..>4716)
                     /locus_tag="VHE37_13870"
     CDS             complement(4035..>4716)
                     /locus_tag="VHE37_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36672.1"
                     /translation="GLDWANRDWGVNLQLPYLQRSHGTLADGDTDVSSSDASGMGDMR
                     ITARYQGFFADRSFGVMAGVKLATGDFHQTFESGPQAGGALDRGLQRGTGTTDGIIGV
                     YHYTALSRDWDLFEQAQFKGALGAREDFRPGNQWIANVGLRYVGAGAWIPQAQLNYKH
                     EAQESGAQADQPNSGSDELYLSPGLAWRASRPLALYGFVQLPLYRRYTGLQLAPEYSV
                     TTGVRYSF"
BASE COUNT          760 a         1588 c         1476 g          892 t
ORIGIN      
        1 aggtagttgc ccaccgtcac cgcctgctgc gcttccgagg cgccgaagtc cagcacgcgc
       61 accgcatcaa gaaattcatg caggagcttg ccgtagcgcg tggtcgcgcg cggatttcca
      121 cgcaggccgg cttcgacctc catgcggccg atggcggaga tcgccacgtc gcacggcttg
      181 agagacgaca gcctgatcgc catcggcagc cgccccttca ccgcggcggc aatgcagtta
      241 ctgtccagca gatatttcac cagcgcaccc ccgcggcggc agcgtccggg cgctgcagca
      301 ccaggtcggc atcgtcgttg gcggcaagct ccggcgcggg attctggaaa tgttccttca
      361 gcgtccgcgg ccagtccggc tcttcgttgc ggtccagcca ttgctgtacg gccagcacga
      421 tgatgcgatt gcgcgtcagc ccggtgcggc cgactgcggc cgcgagccgg tccaacgtcg
      481 cttcatcgaa atgaacactg aaattcatga tgttgaatac taccctaaca tcatatgtta
      541 gatcaatagc gcgatcgtgc cgcaaaagaa aaggcccgcc ggagcgggcc ctttgattca
      601 acggttactc ggcacttccg ccgggtgact cttcggcgga ttcgtccgct tccacgcgat
      661 ccaggcccac caggcgatcg ccctccaccg ggcgcatgat gcgcacgccc tgggtgttac
      721 gtccggccac gcgcacttcc ttcgcacgcg tacggatcag gttgcccgct tccgagatca
      781 gcatgatttc gtggctgtcg tcgaccacca cggcgccaac cagccggccg gtcttgtccg
      841 tcagcgcctg gccgatcacg ccctgcccgc cgcggccctt gcgcggaaag tcttctaccg
      901 gcgtgcgctt gccataaccg aattccgaca cggtgaggat gtcgccggac gtgggcacga
      961 tcagcgcgat cagccgcgca ccatcgctgc cgccattgcc gttctcgatg acttcttcgg
     1021 cttcgtccac cggcgcttcg gctccagctt cctctgcctc ttcctcgccg gccgcgaagg
     1081 acttgatcag ccgcatgccg cgcacgccag tggcgccgcg ccccatcacg cgcactgcgc
     1141 cctcgttgaa gcggatggca cgcccggcgt cagaaatgag aatcacatca tcgctgcccg
     1201 aagtcagcgc cacatcgacc agcgtgtcgt cgacgcgcag gtccaccgcg atgatgccgt
     1261 tggcgcgcac gttggcgaac gcagccagtt cggtcttctt caccgtgccg cggcgtgtgg
     1321 ccatgaaaat gtacttgccg gcgtagaccg gatcgtcgaa cgacttgatc ggcagcaccg
     1381 cgttgatctt ctcggcgcct tccagcggca gcaggttgtt gaacggtttg ccgcgcccgg
     1441 cgcgcgagcc agtcggcagc tcgaacacgc gcagcttgta gcacttgccc gccgaggaga
     1501 agcacagcag cgtgtcgtgg gtatgggcaa tccacaggcg gtcgacgaag tcttcctcct
     1561 tggtcgctgc cgccagcttg ccgcggccgc cgcgcttctg cgcctggtat tcgatcagcg
     1621 gcacggtctt gacgtagccc tcgtgcgaca gcgtgaccac catgtcctgc ggcgcaatca
     1681 ggtcttcgtg ggcgatgtcc agcgcgctct gcgtgatctc gctgcggcgt tcgtcgccga
     1741 actgctcctt cacttctagc agctctgcgc ggatcaccga cagcaggcgc gcttccgagc
     1801 ccaggatgtc gaggtaatca gcaatggcct cgagcagttc cttgaactcg tcgtggatct
     1861 tgtcctgctc cagcccggtg agccggtgca ggcgcaggtc gaggatggcc tgggcctgcg
     1921 cctcggaaag acgatagccg ccttcgacca ggccgagttc gtccggcaga tcttcaggcc
     1981 gcgaggcagc agcaccgctg cgctcgagca tggcggtgac gccgcctggc tgccacaggc
     2041 gcgcaacgag acctgccttg gcctcggccg ggttgggcga cctgcggatc agctcgatga
     2101 tttcatcgat gttggccagt gcaatggaca aaccttccag cacatgggca cgcttgcgcg
     2161 cctcgcgcag caggtagcgc gtgcgccggg tgaccacgtc gcgccggtgt cgcacgaaga
     2221 tttccagcag ctgcttcagc gacagcgtgc gcggctggcc attgtcgatg gccaccatgt
     2281 tgatgccaaa caccacctgc agctgcgtct gctggaacag gttgttgagc accacctcgg
     2341 cgttctcgcc gcgcttgagc tcgatcacca tgcgcatgcc gtccttgtcg gactcgtcgc
     2401 gcagttcact gatgccctcg atgcgcttgt ccttgaccag ctcggcgatc ttctcgagca
     2461 gacgcgcctt gttcacctgg taaggcagct cggtgacgat gatcgcctgc ttgtcgttgc
     2521 cgatcggctc gaactcggtg cgcgcgcgga tcaccacgcg gccgcggccg gtggcgtagg
     2581 cgccgaccaa accgtgcgtg tccagcagca ggccgccggt gggaaagtcc ggagccggca
     2641 ccagcttcat cagcgaagcg agatcgatgg cggggttgtc gatcagcgcc acgcagccgt
     2701 cgatcacctc tgacaggtta tgcggcggga tgttggtggc catgcccacc gcgatgcccg
     2761 aactgccgtt gaccagcagg ttgggaaagc gcgccggcag caccgtgggc tcctgctcgg
     2821 agccgtcgta gttgggctgg aactcgaccg tttccttgtc gatgtctgcc atcaactcgt
     2881 gcgcgatgcg cgtcatgcgg atttcggtgt agcgcatggc cgccgggtta tcgccgtcca
     2941 ccgaaccgaa gttgccctgc ccgtccacca gcaggtagcg cagcgagaat ggctgggcca
     3001 tgcgcacgat ggtgtcgtag atcgcgctgt cgccatgcgg gtggtacttg cccatgaagt
     3061 cgccgacgat gcgcgcggat ttcttgaacg gcttgttgta gtcgttcttc tgctcggcca
     3121 tcgcgtacag cgagcggcga tgtaccggct tgaggccgac gcgcgcatcc ggcagcgcac
     3181 ggcccacgat cacgctcatg gcgtaatcca ggtacgaccg ccgcatctcg tcctcaagat
     3241 tgacggtcag gatttccttg gcaaattcgg tcatgtgctc cgccatccgg cgcctgcgtg
     3301 ggagccggtt tttaggtcgt gattcggggg ttttggcagc gcctgaattc tagcagaaac
     3361 accctgtccc ggccgcgttt cccgctccaa aaaagacccc accgcacccg catccggcat
     3421 gttattaatt attatattac ttaaaatata tttaatatta attaacactg tcgagggcag
     3481 tgcggtcacc ctcgcgggaa ctgtcgcggc atgtccgcgc agggcgcggc atgatatggc
     3541 atgcccctca tgaagcgtct tgcggctctt gccttgctcg cctgtttcgg ctgcgcgcac
     3601 gcggctgcgc cggaggtggg ttcgacagcg ccgccgttct cggcgcagct gctggacggc
     3661 acgcgcttct cgctgcccgc cgggcacgat cgcgtcgtgc tgatcaactt ctgggcgacc
     3721 tggtgcgagc cctgccaggc tgagctcgcg gccttcgccg cgtacatgcg cgcacatcct
     3781 caggcgccat tgaccatcat cgccgtcagc atggacgatc ccgagcggcg cgcgcaggtg
     3841 gcgcaggtgg cggcaaaact gccgtttgcc gtcgccatcg ccgacaacgt caaggccgaa
     3901 cgctacggcc gtatctggcg cttgccgatg agcttcgtcg tcgaccgcgc cggagtgctg
     3961 cgcgtcgatg gcggacgggg cgagcaggtt ccgttcgacc tgcccaccct cgagcgcacc
     4021 atcacgccgc tgcttcagaa cgaatagcgc acgccggtgg tgacgctgta ttccggcgcg
     4081 agttgcaacc cggtgtagcg ccggtacagc ggcagctgca cgaagccgta cagcgccagc
     4141 ggacgcgacg cgcgccaggc caggcccggg ctcaggtaaa gctcgtcgct gccgctgttg
     4201 ggctgatccg cctgcgcgcc ggattcctgc gcctcgtgct tgtagttgag ctgcgcctgc
     4261 ggaatccacg cgcccgcgcc gacgtaacgc aggcccacgt tggcgatcca ctggttgccc
     4321 ggacggaagt cctcgcgcgc ccccagcgcg cccttgaact gcgcctgttc aaacaggtcc
     4381 cagtcgcgcg acagcgccgt gtagtgatag acgccgatga ttccatcggt cgtgccggtg
     4441 ccacgctgca ggccgcggtc gagcgccccg cccgcctgcg gaccgctctc gaaggtctgg
     4501 tgaaaatcgc cggtggccag cttcactcct gccatcacgc cgaagctgcg gtcggcgaaa
     4561 aagccctggt agcgcgccgt gatgcgcatg tcacccatcc cgctggcgtc ggaactggag
     4621 acatcggtat cgccgtcggc gagcgtgccg tggctgcgct gcagataggg cagttgcagg
     4681 ttcacccccc agtcgcggtt ggcccagtcc aagccc
//
LOCUS       DASZZC010000651         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_426835,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000651 DASZZC010000000
VERSION     DASZZC010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426835"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VHE37_13875"
     CDS             <1..427
                     /locus_tag="VHE37_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36673.1"
                     /translation="HEPAAAIDQAYGAALQRAPADAYLWFDRAQVLAAQGRYGQQLET
                     AAARAEALAPASPALRLAMARMGALHWFRSPAELQQLWLRSMRYELRHNQRQFLRSLI
                     HDGEQYWFCASAALELPLDWWCAKVWDYEEAAHISAGAP"
     gene            424..1899
                     /locus_tag="VHE37_13880"
     CDS             424..1899
                     /locus_tag="VHE37_13880"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVT36674.1"
                     /translation="MSGKSGEAALADHWLFVLLLALLAWVPLPWASRSMIAQAGLATA
                     SCTLLALWLMLAIAGKIGVPRGHGRSLATGMLLWAPWLLWLGFQLVPLHCVLLAELSP
                     RALEMHRAAAQAFSENTSGCIAAPLSIAPGETALQLLLSGGYFAVYLLVILIVGTRER
                     VKLMLWTLVLSGLFQALYGMHMSITGLEYGFFEKKTYGLGVATGTFVNRDHYAAYLEL
                     TLAAGIALVLADLGRWRLGHVADFIADLINLVFSAKFRARIAVVAMVAALVMTRSRMG
                     NVAMFAGLASTGVLYTLLRYRRWFIPSLLLFASLLAVDLLVVSNWYGIGAVVERIEQT
                     DLQTESRLLVLKDLEPVVADYLWTGAGMGSFAQAYAPYRDEDIRAYFDHAHNEYAEFQ
                     IETGIVGIALLGLMLFAHLWHGVRVMLNRRTPVYAAAAFASIMAMLTMMAHGWAEFML
                     RTPAVAATLVALMGLGMSVPSRSPRRRRSDEPGDEPAEAAA"
     gene            complement(1793..>3117)
                     /locus_tag="VHE37_13885"
     CDS             complement(1793..>3117)
                     /locus_tag="VHE37_13885"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HVT36675.1"
                     /translation="DCHLLDHVFNDLSPQLWSALPQRALVEGYATPVTCDLFDDPAGQ
                     FEYSRLTFDPSYAVSCQGGPIAAASASGEWADTSYVYNAQSNPTGTRCTVQDYGVAVW
                     GRRAADGFANRPYDNTGVQYGLSALNQGLITAGQFVDLNEKIGGIDIDWNYQPQRSVA
                     DPFALTVAYRGGRVDYPRESAKVPIIDLRGFSPEEIHTDVHSYSMRARLDQANGNHDN
                     QVIWNGGAALFPDPGAFATSFLTMDAWLSAIEADTSSDPLEVKVVRNKPAGAVDACWI
                     GEQKITDAAVCAAAFPYFGTPRIAAGGPLADNIFKCQLKPLDRASYSVSFTDAQWAQL
                     QSAFPTGVCDYAQAPVGWQPTVPWLSYAGGPGGQPLGDAPKSMAISPAQAHTRFGGAV
                     GICTSMLLVLGALVRRRLRQARRQVRRCAGGAVSGWARSCPGPSARRG"
BASE COUNT          465 a         1057 c         1060 g          535 t
ORIGIN      
        1 gcatgaaccg gcagcagcaa tcgaccaggc ctacggcgcc gcgctccaac gcgcaccggc
       61 cgatgcctat ctctggttcg accgcgcgca ggtgctggcc gcgcagggcc gctatggcca
      121 gcagctggaa accgcagcgg cacgcgccga agcactggcg ccggcctcgc ccgcactgcg
      181 cctggcaatg gctcgcatgg gcgcactcca ctggttccgc tccccggccg aactgcagca
      241 gctctggctg cgcagcatgc gttacgagtt gcgtcacaac cagcggcagt tcctgcgctc
      301 gctgattcac gacggcgagc aatactggtt ctgtgcgagc gccgcgctgg aactcccgct
      361 cgactggtgg tgcgccaagg tctgggacta cgaagaggcc gcgcacataa gcgcgggtgc
      421 gccgtgagcg gaaagtccgg cgaagctgcg ctggcggacc attggctgtt cgtcctgctg
      481 ctcgccctgc tggcctgggt gccgctgccc tgggccagcc gcagcatgat cgcccaggcc
      541 gggctggcca ccgcgtcgtg cacactgctc gccctgtggc tgatgctggc catcgcgggg
      601 aaaatcggcg tgccacgcgg gcacggccgt tcgctcgcca ccggcatgct gctgtgggcg
      661 ccgtggctgc tgtggctggg attccaactg gtgcccctgc actgcgtcct gctcgcggaa
      721 ttgtccccgc gcgcgctgga gatgcatcgc gccgctgcgc aggcattctc ggaaaacacc
      781 tccggctgca ttgcagcgcc gctgagcatc gccccgggcg agaccgcgct gcaactgctg
      841 ctgagcggcg gctattttgc cgtctacctg ctggtcatcc tcatcgtggg cacacgcgag
      901 cgcgtgaaac tcatgctatg gacactggtc ctctccggcc tgttccaggc gctctatggc
      961 atgcacatga gcatcactgg cctcgaatac gggttctttg aaaaaaagac ctatggactg
     1021 ggcgtggcca ccggcacctt cgtcaaccgc gatcactacg cggcatatct ggaactcacg
     1081 ctggccgccg ggatcgcgct ggtgctggcc gacctcggcc gctggcggct gggccatgtc
     1141 gccgatttca tcgccgacct gatcaacctg gtgttcagcg ccaagttccg cgcgcgcatc
     1201 gccgtggtgg cgatggtggc ggcgctggtg atgacgcgct cgcgcatggg caacgtcgcc
     1261 atgttcgccg gcctggcctc gactggcgtg ctctatacct tgctgcgcta ccggcgatgg
     1321 ttcatcccca gccttctgct gttcgccagc ctgctggccg tcgatctgct cgtggtgagc
     1381 aactggtacg gcatcggcgc ggtggtcgaa cgcatcgagc agaccgacct gcagaccgaa
     1441 tcgcgcctgc tggtactcaa ggacctggag cccgtggtgg ccgactacct gtggaccggc
     1501 gccggcatgg gcagcttcgc gcaggcgtac gcgccgtatc gcgacgagga catccgcgcg
     1561 tacttcgatc atgcgcacaa cgagtacgcg gaattccaga tcgagaccgg catcgtcggc
     1621 atcgcgctgc tgggactgat gctgtttgcg cacctgtggc acggcgtgcg cgtgatgctc
     1681 aaccggcgca cgccggtcta tgccgcggcg gcattcgcct cgatcatggc catgctgacc
     1741 atgatggcgc acggctgggc cgagttcatg ctgcgcacgc cggcggtggc cgctaccctc
     1801 gtcgcgctga tgggcctggg catgagcgtg cccagccgct caccgcgccg ccggcgcagc
     1861 gacgaacctg gcgacgagcc tgccgaagcc gccgcctgac cagcgcgccg agcacgagca
     1921 gcatcgaggt acagatgccc accgcgccgc caaatcgcgt atgggcctgc gcggggctga
     1981 tcgccatgga tttcggcgca tcgcccagcg gctgcccgcc cgggccgccg gcgtaactga
     2041 gccagggaac cgtcggctgc cagcccaccg gggcctgcgc gtagtcgcag acgccggtgg
     2101 gaaacgccga ctgcagctgc gcccattgcg catcggtgaa gctcacgctg tagctggcgc
     2161 gatccagcgg cttgagctgg cacttgaaga tgttgtccgc cagcggcccg ccggctgcga
     2221 tgcgcggcgt gccgaagtac gggaacgcgg cggcgcagac cgcggcgtcg gtgattttct
     2281 gctcgccgat ccagcaggcg tcgaccgcgc cggccggctt gttgcgcacc actttgactt
     2341 cgagcggatc gctgctggtg tcggcctcga tcgcggacag ccaggcatcc atggtgagga
     2401 acgacgtcgc gaacgcgccg ggatcgggga acagcgccgc gccgccgttc cagatcacct
     2461 ggttgtcgtg gttgccgttg gcctggtcca ggcgcgcgcg catcgagtag ctgtgcacgt
     2521 cggtgtggat ttcctccggc gagaagccac gcaggtcgat gatcggcacc ttggccgatt
     2581 cgcgcggata gtcgacgcgc ccgccgcggt acgccacggt gagcgcaaac ggatcggcca
     2641 cgctgcgctg cggctggtag ttccagtcga tatcgatccc gccgattttc tcgttgaggt
     2701 cgacgaactg cccggcggtg atcaggccct ggttcagcgc gctaagcccg tactgaacgc
     2761 cggtgttgtc gtagggccgg ttggcgaaac cgtcggcggc gcgccgcccc cacacggcca
     2821 cgccgtagtc ctgcaccgtg cagcgtgttc cagtggggtt gctctgcgcg ttgtagacat
     2881 agctggtgtc ggcccactcg ccgctggcgg acgcggcggc gatcggcccg ccctggcagc
     2941 tgaccgcgta ggacggatcg aaggtcaggc gcgagtactc gaactgcccg gctggatcgt
     3001 cgaacagatc gcaggtgacc ggcgtggcgt agccctcgac cagcgcgcgc tgcggcagcg
     3061 cgctccacaa ctgcggcgag aggtcgttga acacgtgatc gagcaggtgg cagtcct
//
LOCUS       DASZZC010000652         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_178604,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000652 DASZZC010000000
VERSION     DASZZC010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178604"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VHE37_13890"
     CDS             <1..615
                     /locus_tag="VHE37_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HVT36676.1"
                     /translation="AGCQVHAFKAPTAYELEHDFLWRTTHHLPPRGLIGVFNRSYYEE
                     VLAVRVHPEYLRPQQLALPAGTALWQQRYQSIREHERHLCANGTTILKFWLHVSREEQ
                     RRRLLARIEEPDKNWKFDAGDLRERGYWRRYQQAYEAMINGTSTPWAPWHVIPADSKS
                     WMRLLVARIVVNCLRGMKLGYPQLAPAQRAQLRSIRQQLQKRGG"
     gene            615..935
                     /locus_tag="VHE37_13895"
     CDS             615..935
                     /locus_tag="VHE37_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36677.1"
                     /translation="MRAQLALCCLLAAGAARAAAEPAVPDEPVPLGTITVIAPPPNEL
                     EQSDRQLESVQQHLPELGNGRGAQPYRAPDYKSSVFARPEDLAGSVVYDVKAENARTD
                     ARAP"
     gene            complement(942..2069)
                     /locus_tag="VHE37_13900"
     CDS             complement(942..2069)
                     /locus_tag="VHE37_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVT36678.1"
                     /translation="MAATAAPRPEPEQQLLRAIAEMQAGKGNAALHDLETLTQKEPNF
                     RLAQLIYGELLTALSGARGGTVADDTTDPRVQELAEEARLRLESEKAIPPAGTAPSNV
                     LKLAPAYKNIIVIDLPRARLYVMENGKDGQLTQQRHNYAAMGKNGYGKQAEGDNRTPL
                     GVYHITGFKPPKLLPAFYGAGAFPLSYPNPWDVFRHRTGAGIWLHGVPPNLYNRPPRS
                     SEGCVTMANDDVVALKPYVEFGQTPVVLSDDVKWLTQGELKADREAWEQRIETWRSKW
                     AALDTDGYLSYYAPDFSVEGMNYAAFARYKHKVNAGKKFIHVKLSDLNLFRYPGAGEP
                     LVLAEFKLDYRSDNFTQTRQKQQFWRQQKNGEWKIFREENR"
     gene            complement(2123..>2312)
                     /locus_tag="VHE37_13905"
     CDS             complement(2123..>2312)
                     /locus_tag="VHE37_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36679.1"
                     /translation="ISKPTHIRVQAVNPQVSVVDATHARVNFSQAYESDSFNDQVNKV
                     LELKLVNGAWKITREYTR"
BASE COUNT          366 a          765 c          778 g          403 t
ORIGIN      
        1 gccggttgcc aggtgcacgc attcaaggcg cccaccgcgt atgaactcga gcatgatttt
       61 ctctggcgca cgacgcacca tttgcccccg cgcggtctaa tcggtgtgtt caatcgcagc
      121 tactacgaag aagtgctggc ggtgcgcgtg cacccggaat acctgcgccc gcagcagctg
      181 gcgctgccgg ccggcacggc gctgtggcag cagcgctacc agtcgatccg cgagcacgag
      241 cggcacctgt gcgccaacgg caccaccatc ctcaagttct ggctgcacgt gtcgcgcgag
      301 gagcagcgcc ggcgcctgct ggcgcgcatc gaggagccgg acaagaactg gaaattcgac
      361 gccggcgacc tgcgcgagcg cggctactgg cgccgctacc agcaggccta cgaggcgatg
      421 atcaacggca cgtcgacgcc gtgggcgccg tggcacgtga tcccggccga cagcaagtcg
      481 tggatgcgcc tgctggtggc gcgcatcgtg gtcaactgcc tgcgcggcat gaagctcggg
      541 tatccgcaac tggcgccggc acagcgcgcg cagctgcgca gcatccggca gcagttgcag
      601 aagcgcggcg gttaatgcgc gcccaactcg ccttgtgctg cctgctcgct gccggtgccg
      661 cgcgcgccgc cgcggaaccc gccgtgccgg atgagccggt gccgctgggc accatcaccg
      721 tgatcgcgcc gccgcccaac gaactggagc aatccgaccg gcagctggag tcggtgcagc
      781 agcatctgcc cgagctgggc aacggccgcg gcgcgcagcc gtaccgggcg cccgactaca
      841 agagctcggt gttcgcacgg ccggaagacc tggccggctc ggtggtctac gacgtgaaag
      901 cggaaaacgc acgcaccgac gcgcgagcgc cgtagcagac ctcagcggtt ttcctcgcgg
      961 aaaatcttcc actcgccgtt tttctgctgg cgccagaact gctgcttctg ccgcgtctgc
     1021 gtgaaattgt cgctgcggta atcgagcttg aactcggcca gcaccagcgg ctcgccggcg
     1081 ccggggtagc ggaacaggtt gaggtcggac agcttcacgt ggatgaactt cttgccggcg
     1141 ttgaccttgt gcttgtagcg ggcaaaggcc gcgtagttca tcccttccac cgagaagtcg
     1201 ggcgcgtagt acgacaggta gccatcggtg tcgagcgcgg cccacttgct gcgccaggtc
     1261 tcgatgcgct gctcccaggc ttcgcggtcg gccttgagtt cgccctgggt cagccacttc
     1321 acgtcatccg acagcaccac cggcgtctgc ccgaattcca catagggctt gagcgcgacg
     1381 acatcgtcgt tggccatggt cacgcagccc tcggacgaac gcggcggacg gttataaaga
     1441 ttcggcggca cgccgtgcag ccagatgccg gcgccagtgc ggtggcggaa cacgtcccac
     1501 ggattcggat acgacagcgg aaacgcgccg gcgccataga acgccggcag caatttgggc
     1561 ggcttgaagc cggtgatgtg gtacacgccc agcggcgtgc ggttatcgcc ttcggcctgc
     1621 ttgccgtagc cgttcttgcc catggccgcg tagttgtgcc gctgctgcgt gagctggccg
     1681 tccttgccgt tttccatcac gtacagccgt gcgcgcggca ggtcgatcac gatgatgttc
     1741 ttgtacgccg gcgccagctt gagcacgttg gacggcgcgg tgcccgccgg tggaatggcc
     1801 ttctcgctct ccagccgcag gcgcgcttcc tcggccagtt cctgcacgcg cggatcggtg
     1861 gtgtcatcgg ccacggtgcc gccgcgggcg ccggacagcg cggtgagcag ttcgccgtag
     1921 atcagctgcg ccaggcggaa attgggttcc ttctgcgtca gcgtctcgag gtcatgcagc
     1981 gcggcgttgc ccttgcccgc ctgcatctcg gcgatggcgc gcagcaactg ctgctcgggt
     2041 tccggccgcg gcgccgccgt ggcggccacg cagaggaatc cgcccccgat gagcgcgagg
     2101 cggagcgcaa cttggtttga catcaacggg tgtattcgcg cgtgatcttc caggcgccgt
     2161 tgaccagctt gagctcgagc accttgttga cctggtcatt gaagctgtcg gactcgtagg
     2221 cctgcgagaa gttgacgcgc gcatgcgtcg cgtcgaccac gctgacctgc ggattcaccg
     2281 cctgcacgcg gatatgcgtg ggcttggaga tg
//
LOCUS       DASZZC010000653         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_247372,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000653 DASZZC010000000
VERSION     DASZZC010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247372"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..>1888
                     /locus_tag="VHE37_13910"
     CDS             141..>1888
                     /locus_tag="VHE37_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36680.1"
                     /translation="MGLVLRRSAWLLPAALVLLLYARTVGYGFAWDDFNYLYHEAGLR
                     DWSFLWRALTRPFLDSYNYFRPLVLISYALEQRAGGSAALSHATNVIIAAANALLVGA
                     LARRSAAQLAPGIDAARAGVFAGLVYAAHPALVEATAWISGRFDLAFTFFGLLLLWCD
                     RAIARTAPRVAACALLFLLAALCKEAALGLALVLPLWQWMHAPADAGAAQRRGDLFAL
                     LGAAAGGLIYLVLRHGALGYLHMDVTTAPYADNVGHAAMVFMALAGYAGLVLLPFGSV
                     GPQHELPPQGIEGFGAQALMGLLVLLLLLALAWRALRGSRRQLLPLCAALALVPALNI
                     VPIEIKQNYMHDRFLQFPLALLSVWLSLSLGSLPARVRGWAGGLALAWTAACALASAS
                     TIGHWQDDLSLWKWAYDRTPRSQTVIDNLQSSLFDAGDYENCRRFSEFIEQQEHGLTP
                     LRRVRYALSLAQVDQGDAAVAQADQALGSPELERTLPSQRASMDAETGWIYLYEGYPE
                     KARERLTEALQLNPSAGEASYYMAATLMVLGDEAGAEPYRERALGLLHPSQQARRAAQ
                     LAGFVETSRQRAAGRA"
BASE COUNT          234 a          648 c          682 g          324 t
ORIGIN      
        1 gacaaccgtt acaacttctc gccggtgcat tacgtcgatg ccgacatcca gcgtattcac
       61 ggcaccaacg agcgcatcag cgtggccgac tacgcgcagg gcgtgcagtg gtaccggcag
      121 ctgctgggca acctgcagtg atggggcttg tgctgcgccg gagcgcgtgg ctgctgcccg
      181 ccgcgctggt gctgctgctg tatgcgcgca ccgtcggcta tggttttgcc tgggacgatt
      241 tcaactacct gtaccacgag gccgggctgc gcgactggag tttcctgtgg cgcgcgctca
      301 cccgcccgtt cctcgattcg tacaactact tccgcccgct ggtgctgatc tcctacgcgc
      361 tggagcagcg cgccggcggc agcgccgcgc tgtcgcatgc gaccaacgtg atcatcgcgg
      421 cggccaacgc gctgctggtc ggcgcgctgg cgcggcgcag cgcggcgcag ctggcgccgg
      481 gcatcgacgc cgcgcgcgcg ggcgttttcg ccggcctggt ctacgcggcg catcctgcgc
      541 tggtcgaggc cacggcgtgg atttccggcc gcttcgatct cgccttcacc ttcttcgggt
      601 tgctgctgct gtggtgcgac cgcgccattg cgcgcaccgc gccgcgcgtg gcggcctgcg
      661 cgctgctgtt cctgcttgcc gcgctgtgca aggaagcggc gctgggtctc gcgctggtgc
      721 tgccgctgtg gcaatggatg catgcgccgg cggatgccgg ggcggcgcag cggcgtggcg
      781 accttttcgc cctgctgggt gcggcggccg gtgggctgat ctacctggtg ctgcgccacg
      841 gcgcgctcgg ctatctgcac atggacgtca ccaccgcgcc ttacgcggat aacgtcggcc
      901 acgcggccat ggtgttcatg gcgctggccg gttatgccgg cctggtgctg ctgccattcg
      961 gcagcgtggg accgcagcac gagttgccgc cgcaaggcat cgaaggtttc ggtgcgcagg
     1021 cgctgatggg cctgctggtg ctgctgctgt tgcttgcgct ggcatggcgc gcgctgcgcg
     1081 gcagccgccg ccagctgctg ccgctgtgcg ccgcgctggc gctggtgccg gcgctgaaca
     1141 tcgtgccgat cgagatcaag cagaactaca tgcacgaccg cttcctgcag ttcccgctgg
     1201 cgctgctgtc ggtctggctg tcgctgtcgc tcggcagcct gcccgcgcgc gtgcgcggct
     1261 gggccggcgg acttgcgctg gcatggacgg ccgcctgtgc gctggcgagc gcgtcgacca
     1321 ttggccactg gcaggatgac ctgtcgctgt ggaaatgggc ctacgatcgc acgccccgct
     1381 cgcagaccgt gatcgacaac ctgcagagct cgctgttcga tgccggcgac tacgagaact
     1441 gccggcgctt ctcggagttc atcgagcagc aggaacacgg gctgacgccg ctgcggcgcg
     1501 tgcgctacgc gctgtcgctg gcgcaggtgg accagggcga tgccgcggtg gcgcaggccg
     1561 accaggcgct cggctcgccg gagctggagc gcaccttgcc ctcgcagcgc gcgtcgatgg
     1621 acgccgaaac cggctggatc tacctctacg aaggctaccc ggaaaaagcg cgcgagcggc
     1681 tgaccgaggc gctgcagctc aacccgtcgg ccggcgaggc cagctactac atggcggcga
     1741 cgctgatggt gctgggcgac gaggccggcg ccgagccgta ccgcgaacgc gcgctggggc
     1801 tgctgcaccc ctcgcagcag gcgcgccgcg cggcgcagct ggcaggcttc gtcgaaacct
     1861 cgcgccagcg tgccgccggg cgcgcgca
//
LOCUS       DASZZC010000654         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1077901,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000654 DASZZC010000000
VERSION     DASZZC010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077901"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VHE37_13915"
     CDS             complement(<1..1013)
                     /locus_tag="VHE37_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HVT36681.1"
                     /translation="MAHQPEPQARDAALNALRHAVLRPACARLCTVQRALKRQFLAMG
                     TLVTVTLPDAARARAAADAALERVQRELAQFGRDGWAWGDGALARFNRSLLDGAAAEV
                     PPALRTLFDLAWNAHCRSGGRFEPRVGELVRLWGFDEPAHVRSAPPAAPEIERALRDL
                     RAAPPYAGNGRYGPAPGVAWDFGAIGKGYAVDRCFELLLQAGFHDASIDAGGNLAVRG
                     RNGRRAWRIGIRDPRSDARRPSLLASLEASDEAVITHGDDQRGFEHGGVRYGHILDPR
                     RGWPARGLRSLTVVHADAAWADAAGSALFVAGADWPRVARELGLTQVLAFDEHARLRA
                     TPQL"
     gene            complement(971..1888)
                     /locus_tag="VHE37_13920"
     CDS             complement(971..1888)
                     /locus_tag="VHE37_13920"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HVT36682.1"
                     /translation="MATVRMRAPAPTPEAPAVQPPPLPVPPQQPALRLVQLMRWRRPP
                     APRAPAPKKNLTATLREWHKKAGLTACVFLAWLACSGILLTRSNDLGFDVRRIDWPWL
                     MKIYGLRAEPPRSGFSDGAHWLAVTRNAALLDGRPLAFALEQPLGLVDGVAGKQPVEF
                     VATPESVLLLGADGSRIDELRSPVLPVSNVRRIGRPRGKVGIVVQDLDAYASYDGGDS
                     WSSIAPAEVQWSSATALSEAQRGALLPYSRPSVLLEQLLIDLHSGRLFGRVGAWIITL
                     VGFGALWLALSGVWMWWRINQNRRRATPR"
     gene            complement(1893..2471)
                     /locus_tag="VHE37_13925"
     CDS             complement(1893..2471)
                     /locus_tag="VHE37_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HVT36683.1"
                     /translation="MPHHPGRSTSLSTSRRTLLRGGIALMVYGLLARAARAGESVWTT
                     YQTPEAFIAETFGGAIPPVKTLELDAAVQAQVAGVFGHAYTQANKLRYWRANGRSAWI
                     LDDLGKQGYQLTTAGFVVKAGVVENAKVLIYRESRGEQVAEPSFLRQLIGAKLAGAQL
                     DRNVDNISGATLSVQMMQRLARLALKLDAIAA"
BASE COUNT          374 a          920 c          913 g          345 t
ORIGIN      
        1 agttgcggcg tggcacgcag tcgcgcgtgc tcatcgaagg ccagcacctg ggtcagcccg
       61 agttcgcgcg caacgcgcgg ccagtccgcg ccggcaacga acagtgcaga gccggcggca
      121 tcggcccagg ccgcgtcggc atgcaccacg gtgagcgagc gcagtccgcg cgccggccag
      181 ccgcggcgcg gatcgaggat atgcccgtag cgcacgccgc catgctcgaa gccgcgctgg
      241 tcgtcgccat gcgtgatcac cgcctcgtcg ctggcctcga gcgacgcgag cagcgatgga
      301 cgccgcgcgt cgctgcgtgg atcacgaatg ccgatgcgcc aggcgcgccg cccgttgcgc
      361 ccgcgcaccg ccagattgcc gccggcgtcg atcgacgcgt cgtggaagcc tgcctgcagc
      421 agcagctcga aacagcgatc gacggcgtaa cccttgccga tggcgccaaa gtcccaggcc
      481 actcccggcg cgggcccgta gcggccgttg cccgcgtagg gtggcgcggc gcgcaggtcg
      541 cgcagcgcgc gctcgatttc cggtgccgcg ggcggcgcgc tgcgcacatg cgcgggctca
      601 tcgaagcccc acaaccgcac cagctcgccg acgcgcggct cgaagcggcc gccgctgcgg
      661 cagtgcgcgt tccaggcgag gtcgaacagc gtgcgcagtg ccggcggcac ctcggccgcg
      721 gcaccatcga gcaggctgcg gttgaaccgg gccaatgcgc cgtcgcccca ggcccagccg
      781 tcgcggccga actgcgcgag ttcccgctgc acccgctcca gcgccgcgtc tgcggcagcg
      841 cgcgcgcgcg cagcatcggg cagcgtcacc gtgaccagcg tgcccatggc caggaactgg
      901 cgcttcaacg cgcgctgcac ggtgcacagg cgcgcgcagg ccgggcgcaa cacggcgtgc
      961 cgcagcgcgt tcaacgcggc gtcgcgcgcc tgcggttctg gttgatgcgc caccacatcc
     1021 acacgccgct gagggcgagc cacagcgcgc cgaagcccac cagcgtgatg atccaggcgc
     1081 cgacgcggcc gaacagccgc ccgctgtgca ggtcgatcag caattgctcg agcagcaccg
     1141 acggccgcga gtacggcagc agcgcgccgc gctgcgcctc gctcagcgcc gtcgcgctgg
     1201 accattgcac ctcggccggc gcgatgctgc tccagctgtc gccgccgtcg tagctggcat
     1261 aggcgtcgag atcctgcacc acgatgccca ccttgccgcg tgggcgcccg atgcggcgca
     1321 cgttcgacac cggcagcacc ggtgagcgca gttcatcgat gcgcgagccg tcggcgccga
     1381 gcagcagcac gctctccggc gtggcgacga attccaccgg ctgcttgccg gccactccat
     1441 cgaccaggcc cagcggctgc tccagtgcga atgccagcgg cctgccgtcg agcagcgcgg
     1501 cgttgcgcgt caccgccagc cagtgcgcgc catcgctgaa gccgctgcgc ggtggttccg
     1561 cgcgcagtcc gtagatcttc atcagccagg gccagtcgat gcgccgcacg tcgaagccca
     1621 gatcgttgct gcgcgtgagc aggatgccgc tgcacgccag ccaggcgagg aacacgcagg
     1681 cggtgagccc ggccttcttg tgccactcgc gcagggtggc ggtgaggttc ttcttcggcg
     1741 ccggcgcgcg cggtgcgggc ggccgacgcc agcgcatcag ctgcaccagg cgcagcgccg
     1801 gttgctgcgg cggcaccggc aacggcggcg gctggactgc aggggcttcc ggtgtcggtg
     1861 ccggtgcgcg catgcggacg gtggccatgc ggtcacgccg cgatggcgtc gagcttgagc
     1921 gccaggcgcg ccaggcgctg catcatctgc accgacagcg tggcgccgga aatgttgtcg
     1981 acgttgcggt cgagctgcgc gcccgcgagc ttcgcaccga tcagctgcct gaggaacgag
     2041 ggctcggcca cctgctcgcc gcgcgactcg cggtagatca gcaccttggc gttctcgacc
     2101 acgccggcct tgaccacgaa gccggcggtg gtcagctggt agccctgctt gcccaggtca
     2161 tcgaggatcc acgcactgcg gccgttggcg cgccagtagc gcaatttgtt cgcctgcgtg
     2221 taggcgtgac cgaacacgcc ggccacctgc gcctgcacgg cagcgtcgag ctcgagcgtt
     2281 ttcaccggcg gaatggcacc gccgaaggtt tcggcgatga acgcttccgg ggtctggtag
     2341 gtagtccaca cgctttcgcc ggcgcgtgcc gcgcgtgcga gcaggccgta gaccatcagg
     2401 gcaatgcctc cacgcagcag cgtgcggcgg gaggtggaga gagatgtgga ccggcccggg
     2461 tgatgcggca ttgcagtcac ggtgtttcct tctggagatg aacagcggga agaaacccgg
     2521 gtccggcggc gccgcaaggg agcggcgccg cc
//
LOCUS       DASZZC010000655         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_178797,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000655 DASZZC010000000
VERSION     DASZZC010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178797"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VHE37_13930"
     CDS             complement(<1..1120)
                     /locus_tag="VHE37_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVT36684.1"
                     /translation="MSRAPASWGRWPRTQQQVQALHSRCDPLSFDDKHSYLPHGLGRS
                     YGDSCLNDGGVLLATRGLDRYISFDAQRGELCCEAGVSLQEIAQLVLPQGWFLPVSPG
                     TQFVTVGGAIANDVHGKNHHRAGSFGRYVLEFELLRSDGSVHLCSPSSNADLYGATIG
                     GLGLTGLIRWARLQLRPVPGPWLTGERIKFGNLGEFFTLARDSDRDYEYTVAWVDCSA
                     GGSARGRGLYSRGNHAAVRSAEPREARLSMPLTPPLSLVNRLSVAAFNRLNYARQRSA
                     VLPQRWHWRPFFYPLDGIENWNRMYGPRGFFQYQCVVPLVHAHAAIDQMLARIARAGE
                     GSFLAVLKLFGDLSSPGWLSFPRAGATLAIDFPNRGART"
     gene            complement(1117..>2237)
                     /locus_tag="VHE37_13935"
     CDS             complement(1117..>2237)
                     /locus_tag="VHE37_13935"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HVT36685.1"
                     /translation="LPLFERVYASDGTVNLSRTRKRDRLVADFGEQGYDYAGNAWADL
                     PVWHDARQAIVVSRSVTLLARARAQARVAQVFADTPPRLLQWPRALRMHQWIKNLLLF
                     LPLLLSQKFTEPKLLLALWGFAAFCLCASSVYLLNDLLDMPSDRLHPRKRLRPFASGE
                     LPALDGLLLAPLLLGMALVIALEISRWFTSVLLVYYASTLAYSMYLKRIPLIDVMLLA
                     GLYTLRIVAGAAAIGVPLTFWLLAFSMFLFLSLGTMKRYTELAMVRDSGAMRAAGRGY
                     HADDLPLLRNQGVAAGYGSVLVLALYINSPQSLMLYRHPHYLWLLCPLLLYWISRAWM
                     KTHRGLMHDDPIVFALRDIPSLTVGVLMAAAVAIAALL"
BASE COUNT          372 a          756 c          826 g          283 t
ORIGIN      
        1 gcgtgcgcgc gccgcggttg ggaaaatcga tggccagcgt cgcgccggcc cgcgggaacg
       61 acagccagcc gggcgaggac aggtcgccga acaatttgag cacggccaga aacgagcctt
      121 cgcccgcacg cgcgatgcgc gccagcatct ggtcgatggc cgcgtgcgcg tggaccagcg
      181 gcaccacgca ctggtactgg aagaagccgc gcggcccgta catgcggttc cagttttcga
      241 tgccgtcgag cgggtagaag aacggccgcc agtgccagcg ctgcggcagc accgcgctgc
      301 gctggcgcgc gtagttcagc cggttgaacg cggccaccga caggcggttg accagcgaca
      361 gcggcggcgt cagcggcatc gacaggcgtg cttcgcgcgg ctcggcggag cgcacggcag
      421 cgtggttgcc acgcgagtag aggccgcgtc cgcgcgcgga cccgcccgcc gagcaatcga
      481 cccaggccac cgtgtattcg tagtcgcggt cggagtcgcg cgccagcgtg aagaattcgc
      541 cgaggttgcc gaacttgatg cgctcgccgg tcagccacgg gccgggcacc ggccgcagct
      601 gcagccgcgc ccagcggatc agcccggtga ggccgagccc gccgatggtg gcgccgtaca
      661 ggtccgcgtt cgacgacggc gagcacagat gcacgctgcc gtcggagcgc agcagttcaa
      721 actccagcac gtagcggccg aagctgccgg cgcggtggtg gttcttgccg tgcacgtcgt
      781 tggcgatggc gccgccgacg gtgacgaact gcgtgcctgg cgacaccggc aggaaccagc
      841 cctgcggcag caccagctgc gcgatctcct gcagcgacac gccggcctcg cagcacagct
      901 cgccgcgctg cgcgtcgaag ctgatgtagc ggtcgaggcc gcgcgtggcc agcagcacgc
      961 cgccgtcgtt gaggcagctg tcgccgtagc tgcggcccag gccgtgcggc aggtagctgt
     1021 gcttgtcgtc gaacgaaagc gggtcgcagc ggctgtgcag cgcctgcacc tgctgctgcg
     1081 tgcgcggcca gcggccccag gacgccggcg cccggctcac agcagcgcgg cgatggcgac
     1141 cgccgcggcc atcagcactc cgaccgtgag gctggggatg tcgcgcagcg cgaacacgat
     1201 cgggtcgtcg tgcatcaggc cgcggtgcgt cttcatccag gcgcggctga tccagtacag
     1261 cagcagcggg cacagcagcc acaggtagtg cgggtggcgg tacagcatca gcgactgcgg
     1321 gctgttgatg tacagcgcca gcaccagcac ggagccgtag ccggcggcca cgccctggtt
     1381 gcgcagcagc ggcaggtcgt cggcgtggta gccgcgtccg gccgcgcgca tggcgccgga
     1441 gtcgcgcacc atggccagtt cggtgtagcg cttcatggtg cccagcgaca ggaacaggaa
     1501 catggagaag gccagcagcc agaaggtgag cggcacgccg atggcggcgg cgccggccac
     1561 gatgcgcagc gtgtacaggc cggccagcag catcacgtcg atcagcggga tgcgcttgag
     1621 atacatggag taggccagcg tgctggcgta gtagaccagc agcaccgagg tgaaccagcg
     1681 cgagatctcc agcgcgatga ccagcgccat gcccagcagc agcggcgcca gcagcaggcc
     1741 gtcgagcgcc ggcagctcgc cgctggcgaa cgggcgcagg cgcttgcgcg ggtgcaggcg
     1801 gtccgacggc atgtcgagca ggtcgttgag caggtagacg ctggaggcgc acaggcagaa
     1861 cgcggcgaaa ccccacagcg ccagcagcag cttgggctcg gtgaacttct gcgacagcag
     1921 cagcggcagg aacagcagca ggttcttgat ccactggtgc atgcgcagcg cgcgcggcca
     1981 ctgcagcagc cgcggcgggg tgtcggcgaa cacctgcgcc acgcgcgcct gcgcacgcgc
     2041 gcgcgccagc agcgtcacgc tgcgcgagac cacgatggcc tggcgtgcgt cgtgccacac
     2101 cggcaggtcg gcccaggcgt tgccggcgta gtcgtagccc tgttcgccga agtcggccac
     2161 cagccggtcg cgcttgcgcg tgcgcgacag gttcaccgtg ccgtcgctgg catacacgcg
     2221 ctcgaacagc ggcagtt
//
LOCUS       DASZZC010000656         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_247493,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000656 DASZZC010000000
VERSION     DASZZC010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247493"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VHE37_13940"
     CDS             complement(<1..587)
                     /locus_tag="VHE37_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT36686.1"
                     /translation="MSEVEAISWERIHGALSAALQPIVDLQTDSVHGYEACMRGPEGA
                     HHLPAALLSLAQRDGCLLQLEQSACAAAMRAFVRQQLGGALFINVSPASLIEPALQQG
                     LLRCASEIGLAAPRLVIELTEQQMFEDYARLRGAILACRNLGFRVALDDLGAGYAGLR
                     AWSELQPDYVKVDRYFVDQIDRDAAKRGFFGSILE"
     gene            731..1864
                     /locus_tag="VHE37_13945"
     CDS             731..1864
                     /locus_tag="VHE37_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36687.1"
                     /translation="MPAETAAAEPLRVRAGAISALRLFDVSYAIDLALLEKLWRDTAR
                     PASTRSRLSTTPAKAIAFDVPPVLLALGTVELKLKSGTVAATASARCSDFGVIALALR
                     VPAAERSWGEFNALMTEVDQAVGPNASIDLWSALLQPLCEQFRAVFVRPGSSLLQEDY
                     LIGIVQQWNRPMSAAAVREQVDLAALLSGEQRPLSEASRRELLSESYSYYEDDLVVLT
                     WDRAFIYEPRQDTDVADVLEVANAQLLEQRYYDELLDDELPRMYDLVEQTRRARYWLA
                     PRRYAHLARRLHMLVAEVTELTEKVDNALQVTEDVYLARVYAAALDTLRVHKIGAAVD
                     RKLAIIRDTYAALYDEASSSRGELMELTIVLLIVLELILALLR"
     gene            complement(1957..>2333)
                     /locus_tag="VHE37_13950"
     CDS             complement(1957..>2333)
                     /locus_tag="VHE37_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36688.1"
                     /translation="SVMMPFAPALRPVYEELVRACAELGLQCRRADDIWDESEVIQDV
                     FSLIWRSAVVICDFSERNSNVFYETGIAHTLGKPVIPILQQRGDAPFDLKHHRYIEYR
                     NSASGRAALARKVGERLRTLLK"
BASE COUNT          386 a          776 c          786 g          385 t
ORIGIN      
        1 atttccagga tggagccgaa gaagccgcgc ttggcggcgt cgcggtcgat ctggtcgacg
       61 aagtagcggt cgaccttgac gtagtccggc tgcagttccg accaggcacg caggccggcg
      121 tagccggcgc ccagatcgtc aagcgccacg cggaaaccca ggttgcggca ggccaggatg
      181 gcgccgcgca ggcgcgcgta atcctcgaac atctgctgct cggtgagctc gatcaccagc
      241 cgtggagcgg ccaggccgat ttccgacgcg cagcgcagca ggccctgctg cagcgcgggc
      301 tcgatcaggc tggccgggct gacgttgatg aacagcgcac cgccgagctg ctgccgcacg
      361 aacgcgcgca tggcggccgc gcaggcactc tgctccagct gcagcaggca gccgtcacgc
      421 tgcgccagcg acagcaacgc ggccggcaga tggtgcgcgc cttccggccc gcgcatgcag
      481 gcctcgtagc cgtgcacgga atcggtttgc aggtcgacaa tgggctgcag cgccgcgctg
      541 agcgcgccgt ggatccgctc ccaagatatg gcttcgacct cggacatgat tgccgcaaac
      601 aaccgcggca ccttttctta ttgctggacc cgttagtttc ccgcctccgc gttgcagccc
      661 ggtgaaggcg gcttcgcgcc ggtgataata cgccgcttgc ggccggggcg aattggagcg
      721 agggtgcgcc atgcccgccg aaaccgccgc cgccgaaccg ctgcgcgtcc gcgccggcgc
      781 catcagcgcg ctgcgcctgt tcgatgtgtc ctatgccatc gatctcgccc tgctggaaaa
      841 gctctggcgc gacaccgcgc gcccggccag cacgcgcagc cggctgtcga ccacgccggc
      901 caaggccatc gccttcgacg tgccgccggt gctgctggcg ctgggcacgg tggagctgaa
      961 attgaagagc ggcacggtcg cagcgacggc ctcggcgcga tgctccgact tcggcgtcat
     1021 cgcgttggcg ctgcgcgtac cggcggcgga gcgctcgtgg ggcgagttca acgcgctgat
     1081 gacggaagtc gaccaggcgg tcggccccaa cgcgtcgatc gatctgtgga gcgcgctgct
     1141 gcagccgctg tgcgagcagt tccgcgcggt gttcgtgcgg cccggcagtt cgctgctgca
     1201 ggaggactac ctgatcggca tcgtgcagca gtggaaccgg ccgatgagcg cggccgcggt
     1261 gcgcgagcag gttgacctcg ccgcgctgct gtcgggcgag cagcgcccgc tgtcggaagc
     1321 cagccgcagg gaactgctca gcgaaagcta ttcgtactac gaggacgacc tggtcgtgct
     1381 cacctgggac cgcgccttca tctacgagcc gcgccaggac accgacgtgg ccgacgtgct
     1441 ggaagtggcc aacgcgcagc tgctcgaaca gcgctactac gacgagttgc tggatgacga
     1501 actgccgcgc atgtacgacc tcgtcgagca gacgcggcgc gcgcgctact ggctggcgcc
     1561 gcggcgctac gcgcacctgg cgcgccggct gcacatgctg gtggcggaag tcaccgagct
     1621 gaccgagaag gtggataacg cgctgcaggt aaccgaggac gtgtaccttg cgcgcgtcta
     1681 cgccgccgcg ctcgacacgc tgcgcgtgca caagatcggc gccgcggtcg accgcaagct
     1741 ggccatcatc cgcgacacct acgccgcgct gtacgacgag gcctccagca gccgcggcga
     1801 gctgatggag ctgaccatcg tgctgctgat cgtgctggag ctgattttgg cgctgctgag
     1861 gtgaccaccg cacccgcttc tggcatgtta ctaattattg tttatataat aaattgatag
     1921 ataattaata acatcgattg tgacggtgcg gtggccttac ttcagcagcg tgcgcagcct
     1981 ctcgccgacc ttgcgcgcca gcgccgcgcg tccgctggcg ctgttgcggt actcgatgta
     2041 gcgatggtgc ttgagatcga acggcgcatc gccgcgctgc tgcaggatcg ggatcaccgg
     2101 cttgcccagc gtgtgcgcga tgccggtttc gtagaacacg ttggagttgc gctcgctgaa
     2161 atcgcagatc accaccgccg agcgccagat cagcgagaac acgtcctgga tcacttcgga
     2221 ttcgtcccag atgtcgtccg cgcgccggca ttgcaggccc agttccgcgc aggcgcgcac
     2281 cagttcctcg tataccggtc gcagcgcggg cgcgaacggc atcatcaccg aca
//
LOCUS       DASZZC010000657         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_820588,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000657 DASZZC010000000
VERSION     DASZZC010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820588"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1189)
                     /locus_tag="VHE37_13955"
     CDS             complement(71..1189)
                     /locus_tag="VHE37_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVT36689.1"
                     /translation="MDIGYFLKLMMDRGASDMFLSTGTPVHIKIEGRLAALGTSPLPS
                     GMVKKIAYSLMDEGSVPKFERELEYNMALAVKDVGRFRVNVFKQRGEVGLVIRAIKSD
                     IPSIEQLLLPPIYKKLIMEPRGLILVVGATGSGKSTTLASMIDHRNTNSAGHILTVED
                     PIEFLHRHKKSIVNQREVGLDTHGYHEALKNAMREAPDVILIGEIRDTDTMDAAIGFS
                     ETGHLCLATLHSNNADQTIERILNFFPEAAHKNILMNLALNLKAIISQRLVVGKDGRR
                     RPAVEVLINTPHIRDLLRRGEVHEIKLAMERSLEEGMQTFDQSLFKLYKEGTIDLEEA
                     LSKADSRDGLALKIRLAEGATGTDLPQDDPYGMGLSSM"
     gene            1192..>1670
                     /locus_tag="VHE37_13960"
     CDS             1192..>1670
                     /locus_tag="VHE37_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36690.1"
                     /translation="MIPGLEGPRRLPRRCKAEMVRRQCADALPQVVFMTRARRKIHDL
                     ALPGLLGLTALLAGCATAPPPIQMMDRAQSEIRAARSAGAATAAPDALAEAERRLAAA
                     QQLSANNDNAKAADKAAEAEAAAATARAQADAARLGDQIQQQSSVNASLQEDLQRRQA
                     "
BASE COUNT          281 a          537 c          546 g          306 t
ORIGIN      
        1 agtatgcgta ttgcttctgt cggttgcagc cgtaggggtt cgcttgtgcg gcagcgaaac
       61 cgctccgcaa tcacatcgac gacaggccca tgccgtacgg atcatcctgc ggcaaatcgg
      121 tgccggtggc gccttcggcc agccggatct tgagcgccag gccgtcgcgc gagtcggcct
      181 tcgaaagcgc ctcttcgaga tcgatcgtgc cttccttgta gagcttgaac agcgactggt
      241 cgaaggtctg catgccttcc tccaggctgc gttccattgc cagcttgatc tcgtgcacct
      301 cgccgcggcg cagcaggtcg cggatgtgcg gggtgttgat cagcacctcg accgcggggc
      361 ggcggcggcc gtccttgccg accaccaaac gttgcgaaat gatcgccttc aggttcagcg
      421 ccaggttcat caggatgttc ttgtgcgcgg cctctgggaa gaagttgagg atgcgctcga
      481 tggtctggtc ggcattgttg gaatgcagcg tcgcgaggca gaggtggccc gtttcggaga
      541 agccgatcgc ggcgtccatg gtgtcggtgt cgcggatttc gccgatcagg atcacgtccg
      601 gcgcctcgcg catcgcgttc ttcagcgcct cgtggtagcc gtgggtgtcg aggcccactt
      661 cgcgctggtt gacgatggat ttcttgtgcc ggtgcaggaa ttcgatcgga tcctcgaccg
      721 tcaggatgtg gccggccgag ttggtgttgc ggtgatcgat catcgaggcc agcgtggtcg
      781 acttgccgga acccgtggcg cccaccacca ggatcagccc gcgcggttcc atgatcagct
      841 tcttgtagat cggcggcagc agcagttgct cgatcgacgg aatgtcgctc ttgatcgcgc
      901 ggatcaccag cccgacctcg ccgcgctgct tgaacacgtt gacgcggaag cggccgacgt
      961 ccttcacggc cagcgccatg ttgtattcga gctcgcgctc gaacttgggc accgagcctt
     1021 cgtccatcag cgaataggcg atcttcttca ccatgccgga aggcagcggc gaagtgccca
     1081 gcgcggccag ccggccctcg atcttgatgt gcacgggcgt gccggtggac aggaacatgt
     1141 ccgacgcacc gcggtccatc atcagcttca ggaaatatcc gatgtccatc attgatcccc
     1201 ggtctggaag gcccgcgcag gctgccgcgg cgttgcaaag ccgaaatggt acgccgacaa
     1261 tgcgccgatg cactccctca agtggtgttt atgacacgcg cacgtcgaaa aatccatgac
     1321 ctcgcgttgc cgggactgtt ggggctcacc gccctgctgg ccggttgcgc gaccgcgccg
     1381 ccgccgatcc agatgatgga ccgcgcgcag tcggaaatcc gcgcggcgcg gagtgcgggg
     1441 gcagcaacgg ccgcgccgga tgccctggcc gaggccgagc gccgtttggc cgccgcacag
     1501 cagctcagcg ccaacaacga caacgccaag gccgccgaca aggccgccga agccgaagcc
     1561 gcggccgcta ccgcacgcgc gcaggccgac gccgcgcgac tcggcgacca gatccagcag
     1621 caatcctcgg tcaacgcgtc gttgcaggaa gacctgcagc ggcgccaggc
//
LOCUS       DASZZC010000658         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1078016,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000658 DASZZC010000000
VERSION     DASZZC010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078016"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..462
                     /locus_tag="VHE37_13965"
     CDS             142..462
                     /locus_tag="VHE37_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36691.1"
                     /translation="MKSRVLSMLLAGVLTACAIGASAAPPRSAAAPPPPAHPASLEQA
                     VRQVQHQTRGHILAADTVSRGQTNVYRIKVLTPQGKVQVMQLHSSPPERGHSSKSKSD
                     QGGH"
     gene            462..1154
                     /locus_tag="VHE37_13970"
     CDS             462..1154
                     /locus_tag="VHE37_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT36692.1"
                     /translation="MRILLVEDEAPLRETLAARLKRDGFAVDAAADGEEGLYLGREVP
                     FDLAIVDLGLPKLSGMELIEILRKEGHSYPVLILTARSSWQDKVEGLKFGADDYLVKP
                     FHVEELLARINALVRRASGWAKPVLSCGPIRLDTTAQTVTVEGKPTDLTSYEYKVLEY
                     LMLHAGELVSKADLTEHIYQQDFDRDSNVLEVFIGRLRRKLDPESSLKPIETVRGRGY
                     RFAIARDETPAE"
     gene            1256..>2044
                     /locus_tag="VHE37_13975"
     CDS             1256..>2044
                     /locus_tag="VHE37_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36693.1"
                     /translation="MAEDTASRRPLSLVSRSIIATLLVLVGFLGITGFALDRAYKHAA
                     LEALRAQLQSYAYTYLSGIDVSSRSERVIPPDVTPPNPDFGRPGSGLYAVIVGQGGFH
                     WEAQSALGRDLPFDDLLPPGPAKFEGPVDTSAGPLYVLSQGIDWIPLQSGTGYHLTIY
                     IAENDALFHTGLAAYRRTLFGWLAALGLALLVLQQILLRWSFSPLRRVARDIARIERG
                     SAERLVGPYPREVEVLTRGLNRFIESEREHLKRYRNTLDDLEHSL"
BASE COUNT          343 a          678 c          674 g          349 t
ORIGIN      
        1 gctcgatgaa gtgcgcttgc ccggcgagcg gcgagccgcc tgacggcggc gcctggcctg
       61 tcgccggcgg catgttcagc acgaattcaa tgggtggccg gatgatcggg ggctgaagtt
      121 ttacccgagc ccgcgtcggc catgaaatcc cgcgtccttt ccatgctgct ggctggcgtc
      181 ctcacggcgt gcgccatcgg tgcttccgca gcgccgccgc gttcggccgc cgcgcctccg
      241 ccgcccgccc atccggccag cctggagcag gccgtgcggc aggtgcagca ccagacccgc
      301 gggcatatcc ttgcggccga tacggtgtcg cgcggccaga ccaacgtgta tcggatcaag
      361 gtgctgacgc cgcagggcaa ggtgcaggtg atgcaactgc attcgagccc gccggagcga
      421 ggccactcga gcaagtccaa atccgatcaa ggaggacact gatgcgtatc ctgctggtgg
      481 aagacgaagc gcccctgcgt gaaacgctgg cggcgcgcct gaagcgcgac ggtttcgcgg
      541 tggatgcggc cgccgatggc gaggaagggc tttacctcgg gcgcgaagtg ccgttcgatc
      601 ttgcgatcgt ggatctcggc ctgcccaagt tgtccggcat ggaactgatc gagatcctgc
      661 gcaaggaagg ccacagctat ccggtgttga tcctcaccgc gcgttcctcg tggcaggaca
      721 aggtggaagg gctgaaattc ggcgccgacg actacctcgt caaaccgttc cacgtcgagg
      781 aactgctggc gcgcatcaac gcgctggtgc ggcgcgcgtc cggctgggcc aagccggtgt
      841 tgtcgtgcgg accgatccgg ctcgacacca ccgcgcaaac ggtcacggtc gaaggcaagc
      901 ccaccgacct gaccagctac gaatacaagg tgctggaata cctgatgctg cacgccggcg
      961 aactggtgtc gaaggccgat ctcaccgaac acatctacca gcaggatttc gaccgcgatt
     1021 cgaacgtgct ggaagtgttc atcggccgcc tgcgccgcaa gcttgatccg gaaagcagcc
     1081 tgaagccgat cgagacggtg cgcgggcgcg gctaccgctt cgcgatcgcg cgcgacgaaa
     1141 cgcccgccga atgaggcgcc gtccgcggcg gcgccggcag ttgcacgcgc ccggttcctg
     1201 cggtgatgat ggtgatcctt gcggtcggct gtgactgcac gaaaggtctt gccggatggc
     1261 tgaggacacc gcctcgcgca ggccgctgtc gctggtctcg cgctccatca tcgcaacctt
     1321 gctggtgctg gtcggcttcc tcggcatcac cggtttcgcg ctcgaccgcg cgtacaagca
     1381 tgcggcgctg gaagccttgc gcgcccagct gcaaagctac gcctacacgt atctttccgg
     1441 catcgacgtg agcagccgct ccgaacgggt gatccctccg gacgtgacac cacccaaccc
     1501 cgatttcgga cggccgggtt ccggtctgta cgcagtgatc gtggggcagg gcggcttcca
     1561 ttgggaagcg caatcggcgc tcggccgcga tcttcccttc gatgatctgc tgccgcccgg
     1621 cccggcaaag ttcgaggggc cggtcgacac ttcggcgggt ccgctgtacg tcctgagcca
     1681 gggcatcgac tggattccgc tgcaaagcgg cacgggctac cacctcacca tctacatcgc
     1741 cgaaaacgac gccctgttcc acaccggtct cgccgcctac cggcgcacgc tgttcggctg
     1801 gctggccgcg ttgggattgg cgctgctggt gctgcagcag atcctgttgc gctggagttt
     1861 ttcgccgctg cgccgggtgg cgcgcgacat cgcgaggatc gagcgcggca gcgccgaacg
     1921 cctggtcggg ccgtatcctc gcgaagtcga agtgctgacg cgcggactca accgtttcat
     1981 cgaaagcgag cgcgaacacc tgaagcgcta ccgcaacacc ctcgacgacc tggagcacag
     2041 cctg
//
LOCUS       DASZZC010000659         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_377194,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000659 DASZZC010000000
VERSION     DASZZC010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377194"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VHE37_13980"
     CDS             <1..205
                     /locus_tag="VHE37_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT36694.1"
                     /translation="AKTTPHMFIIDPAGKLIYAGGIDSIASTDIDDLKQAKPYVKLAL
                     DDALAGKPVSIASTRPYGCSIKY"
     gene            complement(170..1549)
                     /locus_tag="VHE37_13985"
     CDS             complement(170..1549)
                     /locus_tag="VHE37_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HVT36695.1"
                     /translation="MNAVLACILLYILLQLVIAFWFSRRNRNEEDYLLAGRSLGPWMA
                     TFTVFATWFGAETCIGAAGEAYGSGLSGVLADPFGYTAGIVLMGFFFAAALWKRGLVT
                     LADLFRQRYGGGVERLASIIMIPSSVMWAAAQIRAFGQVLASASELGLFAAITLAAAV
                     VIAYTAVGGMWADAVTDLLQGIVLIAGVLALGVVFVQLGGLHVLAALPPERVSFTAGR
                     SWASVFDTLAVPVFSTIAAQELASRVLAMRSQQLARSATVVAGLMYLVIGGVPVLIGL
                     GAAAYIGTGANAEQVLALFAQKQLSLPLYILFLGALVSAILSTLSGALLVAGSLAAHN
                     VVAPLVPGMTDAAKLKANRIAVITFGVIAYGIALASESVYTLVQESSGLGSSGILVIM
                     LFALWGARVGRAMSAYVALVAGTVTFIAATHLFAWDYAYLASLAAALAAYLLCAPLSV
                     LDRAAVGPG"
     gene            complement(1574..1795)
                     /locus_tag="VHE37_13990"
     CDS             complement(1574..1795)
                     /locus_tag="VHE37_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36696.1"
                     /translation="MNVFEMVALIVLVSAIAAVAKAWIKSQHAVPAQPSPEQERRMAQ
                     LEQRVQALEAVVTDHGYDLRKEFSKLERG"
     gene            1967..>2224
                     /locus_tag="VHE37_13995"
     CDS             1967..>2224
                     /locus_tag="VHE37_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HVT36697.1"
                     /translation="MRKTSGKSASPVRLAIHGGAGDLPPNAPPQREHEAALARIAGEG
                     RALLQDGAGALDAVERMVELLEQCPLFNAGIGAVLNRDGLPE"
BASE COUNT          413 a          773 c          729 g          309 t
ORIGIN      
        1 tgccaagacc acgccgcaca tgttcatcat cgatccggcc ggcaagctga tctacgccgg
       61 cggcatcgac agcattgcca gcaccgacat cgacgacctc aagcaggcca agccctacgt
      121 gaagcttgca ctggacgacg cgctggcggg caagccggtg agcatcgcct caacccggcc
      181 ctacggctgc tcgatcaagt actgatagcg gcgcgcagag caggtacgcg gccagcgcgg
      241 cggcgagcga ggcgaggtag gcgtagtccc acgcaaacag gtgcgtggcg gcgatgaagg
      301 tgacggtgcc ggcgaccagc gccacgtagg cgctcatggc gcgtcccacg cgcgcgcccc
      361 acagcgcgaa tagcatgatg accaggatgc cggacgagcc gagccccgac gattcctgca
      421 ccagcgtata cacgctttcg ctcgccagcg cgatgccgta ggcgatgacg ccgaacgtga
      481 tcaccgcgat gcggttggcc ttcagcttgg cggcgtcggt catgcccggc accagcggcg
      541 cgaccacgtt gtgcgcggcc agcgaaccgg ccaccagcag cgcgccggat agcgttgaaa
      601 gaatcgccga gaccagtgcg ccgagaaaca ggatgtacag cggcagcgac agctgcttct
      661 gcgcgaacag tgccagcacc tgctcggcgt tggcgccggt gccgatatac gccgccgccc
      721 ccaggccgat cagcaccggc acgccgccga tcaccaggta catgaggccg gccaccaccg
      781 tcgcgctgcg cgccagctgc tggctgcgca tggcgagcac gcgcgaggcc agctcctgcg
      841 cggcgatggt ggagaagacc ggcaccgcca gcgtgtcgaa cacgctggcc cagctgcgcc
      901 cggcggtgaa gctgacgcgc tcgggcggca gcgcggccag cacgtgcagc ccgccgagct
      961 gcacgaagac aacgcccagc gcgagcacgc cggcgatcag cacgatgccc tgcagcaggt
     1021 cggtgaccgc atcggcccac atgccgccga ccgcggtgta ggcgatgacc acggcggccg
     1081 cgagcgtgat ggcggcgaac aggccgagtt cggacgcgga cgccagcacc tggccgaacg
     1141 cgcggatctg cgccgcggcc cacatcaccg acgagggaat catgatgatc gacgccagcc
     1201 gttccacgcc gccgccgtag cgctggcgga acaggtcggc cagcgtcacc agtccgcgtt
     1261 tccacagcgc ggcggcgaag aagaagccca tcagcacgat gcccgcggtg tagccgaatg
     1321 gatccgccag cacgccggac aggcccgagc catacgcttc gcccgcggcg ccgatgcagg
     1381 tctccgcgcc gaaccaggtg gcgaacacgg tgaaggtggc catccacggg ccgaggctgc
     1441 gcccggcgag caggtagtcc tcctcgttgc ggttgcgccg cgagaaccag aaagcgatga
     1501 ccagctgcag caggatgtag agcaggatgc aggcgaggac cgcgttcatg cgcgcgccgc
     1561 tccgcgcagg cgctcagccg cgctcaagct tgctgaattc cttgcgcagg tcatagccat
     1621 ggtcggtgac gaccgcctcg agcgcctgca cgcgctgctc cagctgcgcc atgcgccgct
     1681 cctgctcggg cgagggctgc gccggcacgg catgctgcga cttgatccag gccttggcca
     1741 ccgcggcgat cgcgctcacc agcacgatca gcgccaccat ctcgaacacg ttcatcgctt
     1801 gctccatcga ctcgcgcgct agtctaaagg cctccaccgc atgccgtttt tgcgtgttat
     1861 ataatattgt ttaattttat atttatgtta ttatttataa catcaaaaaa tgccgtgcgg
     1921 taacagcaaa aatcgaccgg tgcgcctctg ctagagtcgc cgcagcatgc gcaagacctc
     1981 cggtaaatct gcttcgcccg ttcgcctggc catccacggc ggcgccggcg acctgccgcc
     2041 caatgcgccg ccgcagcgcg agcatgaagc ggcgctggca cgcatcgccg gcgaaggccg
     2101 cgcgctgctg caggatggcg ccggcgcact cgatgcggtg gagcggatgg tcgaactgct
     2161 ggagcagtgc ccgctgttca acgccggcat cggcgcggtg ctcaaccgcg acggcctgcc
     2221 cgag
//
LOCUS       DASZZC010000660         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_178980,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000660 DASZZC010000000
VERSION     DASZZC010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178980"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VHE37_14000"
     CDS             complement(<1..550)
                     /locus_tag="VHE37_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HVT36698.1"
                     /translation="MTDSNEPRRPTPDELLAQTQREARGKLKIFLGAAPGVGKTWEML
                     SSARKKKNDGVDVVIGVVETHGRRETLALVAGLEVLPRRKVEYKGHTLEEMDLDALLA
                     RKPRIALVDELAHTNAPGSRHPKRYQDVEELLAAGIDVYSTLNIQHVESLNDVVAQIT
                     RVRVRETVPDSILDAADEVEIID"
     gene            complement(563..1162)
                     /gene="kdpC"
                     /locus_tag="VHE37_14005"
     CDS             complement(563..1162)
                     /gene="kdpC"
                     /locus_tag="VHE37_14005"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HVT36699.1"
                     /translation="MTTHFRPALVLLVLFTLLLGFAYPMAISGLGALLFPRQAAGSLI
                     ERDGRVIGSALVGQVFTGEKYFHPRPSAISGTDPNDSTKTVGTPYDASNSMGSNAGPT
                     AKSLIDRVTADSAALKAENPSAPVPVDLATASSSGLDPHISLAAALFQLPRVARARQL
                     DQAKLKALVDAHLQGRWLGIFGEPVVNVLELNLALDQGG"
     gene            complement(1175..>1559)
                     /locus_tag="VHE37_14010"
     CDS             complement(1175..>1559)
                     /locus_tag="VHE37_14010"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647851.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HVT36700.1"
                     /translation="TKLIEIVAIGKQLLMTRGALTTFSIANDVAKYFAIIPAMFAAIY
                     PQLAPLNVMHLATPQSAILSAIIFNALIIIALIPLALRGVHYRPVGAGQLLSRNLLIY
                     GVGGVIAPFIGIKLIDAMVAAAGLA"
BASE COUNT          254 a          503 c          529 g          273 t
ORIGIN      
        1 ggtcgatgat ctcgacctca tcggccgcat cgagtatcga gtcgggcacc gtttcgcgca
       61 cgcgcacgcg ggtgatctgc gccaccacgt cgttgaggct ctcgacatgc tggatgttga
      121 gcgtgctgta gacgtcgatg cccgcggcca gcagttcctc gacgtcctgg tagcgcttgg
      181 gatgccggct gcccggtgcg ttggtatgcg ccagttcgtc gaccagcgca atgcgcggct
      241 tgcgcgcgag cagcgcatcg aggtccatct cctcgagcgt gtggcccttg tactccacct
      301 tgcgccgcgg cagcacttcg agtcctgcga ccagcgccag cgtttccctg cgcccgtgcg
      361 tctcgaccac gccgatgacc acgtcgacgc cgtcattttt cttcttgcgc gccgacgaca
      421 gcatctccca ggtcttgccc acgccgggcg cggcgccgag aaagatcttc agcttgccac
      481 gcgcttcgcg ctgcgtctgc gccagcagtt cgtcaggagt cggtcgccgc ggctcgttgc
      541 tgtcggtcat tgcaccagga ttttaccccc cttgatcgag cgcgagattg agttccagca
      601 cgttgaccac cggctcgccg aaaatgccga gccagcggcc ctgcaggtgc gcgtcgacca
      661 gcgccttcag tttcgcctgg tcgagctggc gcgcgcgcgc cacgcgcggc agctggaaca
      721 gcgcggcggc caggctgata tgcggatcga gcccgctgga ggaagccgtg gccagatcca
      781 ccggcaccgg cgccgacggg ttttccgcct tcagcgcggc actgtcggcc gtgacccggt
      841 cgatcagcga tttcgccgtc gggccggcgt tggagcccat cgagttggag gcgtcgtacg
      901 gcgtgcccac ggtcttggtg gagtcgttgg ggtcagtgcc gctgatcgcc gacggccgcg
      961 gatggaagta tttctcgccg gtgaacacct ggccgaccag cgccgagccg atcacgcggc
     1021 cgtcgcgttc gatcaatgaa ccggcggcct gccgcggaaa cagcagcgcg cctagtccgg
     1081 agatggccat ggggtaggcg aagcccagca gcagggtgaa cagcaccagc agcacgaggg
     1141 cggggcggaa atgtgtggtc atggaaatag tccttcaggc caggccggca gcggcaacca
     1201 tcgcatcgat cagcttgatg ccgatgaaag gcgcaatgac accgcccacg ccgtagatca
     1261 gcaggttccg cgacagcagc tggccggcgc cgaccgggcg gtaatgcacc ccgcgcagcg
     1321 ccagcgggat cagcgcgatg atgatcagcg cgttgaagat gatcgccgac aggatggcgc
     1381 tctgcggggt ggccaggtgc atcacattga gcggcgccag ctgcggatag atggcggcga
     1441 acatcgccgg gatgatggcg aagtacttgg ccacgtcgtt ggcgatcgag aaggtggtca
     1501 gggcgcccct ggtcatcagc agctgcttgc cgatggcgac gatctcgatc agcttggtc
//
LOCUS       DASZZC010000661         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_308765,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000661 DASZZC010000000
VERSION     DASZZC010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308765"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VHE37_14015"
     CDS             complement(<1..540)
                     /locus_tag="VHE37_14015"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648867.1"
                     /note="catalyzes the transfer of a segment of a
                     1,4-alpha-D-glucan chain to a primary hydroxy group in a
                     similar glucan chain; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HVT36701.1"
                     /translation="MDDAKPDDLGHAQALAQGTHGDPFAFLGPHRQGSQLSVRAFVPG
                     AVAVRALDAQTQQPLADLSAGHIGGIYTGHLPADARYLLEIDWGGVLQMTEDPYSYGT
                     LLGDLDLYLIGEGNHRELGRVLGAQVMRVGEVHGVRFAVWAPNARRVSVVGDFNAWDG
                     RRHPMRNRGGVWEIFIPRLA"
     gene            complement(533..>3579)
                     /gene="treS"
                     /locus_tag="VHE37_14020"
     CDS             complement(533..>3579)
                     /gene="treS"
                     /locus_tag="VHE37_14020"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HVT36702.1"
                     /translation="MADVKRFIAAAHKRGLRIITELVINHTSDQHPWFQRARRAKPGS
                     VARNFYVWSDTDQKYQGTRIIFIDTERSNWTWDPVANAYYWHRFYSHQPDLNFDNPHV
                     VKAVLGIMHFWLDLGVDGLRLDAVPYLVEREGTSNENLPETHAILKRIRAEIEARYPD
                     RMLLAEANQWPEDVQEYFGAGDECHMAFHFPLMPRMYMAVAQEDRFPITDILRQTPDI
                     PSNCQWAIFLRNHDELTLEMVTDKERDYLWSKYASDNRARLNLGIRRRLSPLLERDRR
                     RIELLNSLLLSMPGTPVLYYGDELGMGDNIYLGDRDGVRTPMQWSSDRNGGFSRADPA
                     TLVLPPIMDSLYGYDAINVEAQARDPHSPLNWMRRMLALRSQHKAFGRGSMKVLYPNN
                     RHLLAYLRQHSGDDGQEETILCVANVGRSAQAVELDLSSFAGRIPVEMIGGSAFPPIG
                     QLPYLLTLPPYGFYWFMLAAQAHMPSWYVAPPEPLPELATLVVRNSIDEVAKKPLKQI
                     IENDLLPSYLLRRRWFAAKNEKIERACIAQATLLPETRPPVLWTEIEVKAGGELSRYS
                     LPLGLLPDEDQVTALPHQLALSRLRQKHRVGHLTDAYALNTLPRTIIRFLRAGRTLRA
                     DDGELRFLPTSQLERVQVADDAEVRRLSAEQSNSSLIVGDQFMFKLLRRLNPGAHPEA
                     EMGRVLTERGYANIAPLLGEVTRVAGDGTPWVLAVVQGYLHNQGDAWNWLLALIERAL
                     AEIALPADGSQRESAAEMRKDAEDFAAAIGKRLGEMHAVLAQESADDAFAPKISTAAD
                     TRAWARAAAQQIEQACTQLGKAREQLSESERKCAEQIVASRARLVEAAQKLAAGAAGS
                     LMTRTHGDLHLGQILVAQEDAYFVDFEGEPARPLAERRAKTSPLRDVAGMLRSFDYAA
                     AQALNAHPGATLQQREFVDQLRDGVITAFSAAYERAAAVIAHRWSSDKTGPRAALDLF
                     LLEKGAYEICYEAANRPAWLMVPLRGLAAIAKRLGALEAHHG"
BASE COUNT          531 a         1208 c         1245 g          595 t
ORIGIN      
        1 cgccaggcgc gggatgaaga tctcccacac gccgccgcgg ttgcgcatcg ggtggcggcg
       61 cccgtcccac gcattgaaat ccccgaccac cgacacgcgc cgcgcattgg gcgcccacac
      121 ggcgaagcgc acgccgtgca cctcgcccac gcgcatgacc tgcgcgccaa gcacgcggcc
      181 gagttcgcgg tggttgcctt cgccgatcag gtacaggtcg aggtcgccga gcagcgtgcc
      241 gtacgagtaa gggtcctcgg tcatctgcag cacgccgccc cagtcgatct ccagcaggta
      301 gcgcgcatcg gccggaaggt gtcccgtgta gatgccgccg atgtggcccg cagagagatc
      361 ggcgagcggc tgctgtgtct gcgcgtcgag cgcgcgcacc gccaccgcgc ccggcacgaa
      421 cgcgcgcacc gacagttgcg acccctgccg gtgcgggccg agaaaggcga acggatcgcc
      481 atgggtgcct tgggccagcg cctgcgcatg gccgaggtcg tcgggcttgg cgtcatccat
      541 ggtgcgcttc cagtgcgccg agacgcttgg caatcgccgc cagtccgcgc agcggcacca
      601 tcagccaggc cggccggttc gccgcctcgt agcagatctc gtaggcgccc ttctccagca
      661 ggaacaggtc gagcgcggcg cgcggaccgg tcttgtcgct gctccagcga tgcgcaatga
      721 cggcggcggc gcgctcgtag gcggcgctga acgcagtgat gacgccgtcg cgcagctgat
      781 cgacgaactc ccgctgctgt agcgtggcgc ccgggtgcgc gttgagcgcc tgcgccgcgg
      841 cgtagtcgaa gctgcgcagc atgccggcca cgtcgcgcag cggactggtc ttggcgcggc
      901 gctcggccag cggccgcgcc ggctcgcctt cgaagtcgac gaagtaggcg tcttcctgcg
      961 ccaccaggat ctggccgaga tgcaggtcgc catgcgtacg ggtcatcagc gaccccgctg
     1021 cgccggccgc gagtttctgc gcggcctcga ccaggcgcgc gcgcgaggcg acgatctgct
     1081 cggcgcactt gcgctcgctc tcggacagct gttcgcgcgc cttgcccagc tgcgtgcagg
     1141 cctgctcgat ctgctgcgcc gccgccctgg cccaggcgcg cgtgtccgcg gcggtggaga
     1201 tcttcggcgc gaatgcgtcg tcggcggatt cctgcgccag cacggcgtgc atctcgccga
     1261 gccgcttgcc gatggcggcg gcgaaatctt cggcgtcttt cctcatctcc gcggcgctct
     1321 cgcgctggct gccatcggcc ggcagcgcaa tttccgccag cgcgcgttcg atcagcgcca
     1381 gcagccagtt ccacgcatct ccctggttgt gcaggtaacc ctgcaccacg gcgagcaccc
     1441 acggcgtgcc gtcgccggcc acccgcgtca cttcgcccag cagcggcgcg atgttggcgt
     1501 agccgcgctc ggtcagcacg cggcccattt ccgcctcggg atgcgcgccg gggttgagcc
     1561 ggcgcaacag cttgaacatg aactggtcac cgacgatcag cgaactgttc gactgctcgg
     1621 ccgacaggcg ccgcacctcg gcgtcgtccg ccacctgcac gcgttccagc tgcgaggttg
     1681 gcaggaagcg caactcgccg tcatcggcgc gcagggtcct gcccgcgcgc aggaagcgga
     1741 tgatggtgcg cggcagcgtg ttgagggcgt aggcatcggt gagatggccc acgcgatgct
     1801 tctgccgcag ccgcgacagc gccagctggt gcggcagcgc ggtcacctgg tcctcgtcgg
     1861 gcagcaggcc cagcggcagc gagtagcgcg acagttcgcc tccggccttc acctcaatct
     1921 ctgtccacag caccggcggc cgggtctccg gcagcagcgt ggcctgcgcg atgcaggcgc
     1981 gctcgatctt ctcgttcttc gccgcgaacc agcggcggcg cagcaggtac gagggcagca
     2041 ggtcgttctc gatgatctgt ttcagcggct tcttcgccac ctcgtcgatg ctgttgcgca
     2101 cgaccagcgt ggccagttcg ggcaggggct cgggcggcgc cacgtaccag gacggcatgt
     2161 gcgcctgcgc ggcgagcatg aaccagtaaa agccgtacgg cggcagggtc agcaggtacg
     2221 gcagctggcc gatgggcgga aacgcggagc cgccgatcat ctccaccggg atgcggccgg
     2281 cgaacgacga caggtccagt tccaccgcct gcgccgagcg tccgacgttg gccacgcaca
     2341 ggatggtttc ctcctggccg tcgtcgccgc tgtgctggcg caggtaggcc agcaggtgcc
     2401 ggttgttcgg gtagagcact ttcatgctgc cgcggccgaa ggccttgtgc tggctgcgca
     2461 gggcgagcat gcggcgcatc cagttgagcg gcgagtgcgg atcgcgcgcc tgcgcctcga
     2521 cgttgatggc atcgtagccg tagagcgagt ccatgatcgg cggcagcacc agcgtggccg
     2581 ggtcggcgcg cgaaaacccg ccgttgcggt cgctggacca ttgcatcggc gtgcgcacgc
     2641 cgtcgcggtc gcccagatag atgttgtcgc ccatgcccag ttcgtcgccg tagtacagca
     2701 ccggcgtgcc cggcatcgac agcagcaacg agttgagcag ctcgatgcgc cggcggtcgc
     2761 gttccagcag cggcgacagg cgccggcgga tgcccaggtt cagccgcgcg cggttgtcgc
     2821 tggcgtactt gctccagaga tagtcgcgct ccttgtcggt gaccatctcc agcgtgagtt
     2881 catcgtggtt gcgcaggaaa atggcccact ggcagttcga tggaatgtcc ggcgtctgcc
     2941 gcaggatgtc ggtgatgggg aagcggtctt cctgcgccac cgccatgtac atgcgcggca
     3001 tcagcgggaa gtggaaggcc atatggcatt cgtcgcccgc gccgaaatat tcctgcacgt
     3061 cttccggcca ttggttggcc tcggccagca gcatgcggtc ggggtagcgc gcctcgatct
     3121 cggcgcggat acgcttcagg atcgcatgcg tctccggcag gttctcgttg ctcgtgcctt
     3181 cgcgctcgac caggtagggc accgcgtcga gacgcagccc gtcgacaccg aggtcgagcc
     3241 agaaatgcat gatgcccagc accgccttga cgacatgcgg gttgtcgaag ttgaggtccg
     3301 gctggtgcga atagaaacgg tgccagtaat aagcgttggc gaccgggtcc caggtccagt
     3361 tcgagcgctc cgtgtcgatg aagatgatgc gtgtgccctg gtacttctgg tcggtgtccg
     3421 accagacata gaagttgcgc gcgacggaac ctggcttggc gcgccgggcg cgctggaacc
     3481 agggatgctg atccgaggtg tggttgatga ccaactcggt gatgatgcgc agcccgcgct
     3541 tgtgcgccgc ggcgatgaag cgctttacat cggccatcg
//
LOCUS       DASZZC010000662        10101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_377502,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000662 DASZZC010000000
VERSION     DASZZC010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377502"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VHE37_14025"
     CDS             complement(<1..982)
                     /locus_tag="VHE37_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HVT36703.1"
                     /translation="MKRVSVDIGGTFTDCFVVWDNQYIEGKALTTHHNLALGFNEALG
                     NACKDLKLEVNTLLSQVDSVRYATTLGTNALIERKGPRIGVLVTAGFKSTIPLSRARG
                     YGEGLPEERQMDIPNARRPEPIVPIPMIREIRERVDYLGKVFLTLNEDDVRLKIRELV
                     DAGAEALVVLFTNSVVNNAHELRVREIFLEEYPGHLLGAIPMLLSHQVAGRKGEYARG
                     MSTIMDAFLHQTMYHGLGTLEQNLRAAGYTRPMLVNHNSGGMAQLNSTDALQTVHSGP
                     VSGIAASEHLMQQTGLGNVVATDMGGTSFDIGIVDQGGVKHYDFNPVIDRW"
     gene            complement(995..1414)
                     /locus_tag="VHE37_14030"
     CDS             complement(995..1414)
                     /locus_tag="VHE37_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HVT36704.1"
                     /translation="MRVLITEYLRINLDNEQWECRRCGKELGSARGNYKRGLLVYDRD
                     PREIHKPLLDTKKYERTYSPDPTWCRILEYYCPQCGTMCEAEYLPPGHPPLHDIELDI
                     DALKLQWKDKPEVKDVTPGPYIRPPRLEHKHSHGHKH"
     gene            complement(1436..2344)
                     /locus_tag="VHE37_14035"
     CDS             complement(1436..2344)
                     /locus_tag="VHE37_14035"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36705.1"
                     /translation="MRAATHKFMARWGLKPLLRPPFPLWLQRAVAAAGLWLNSRVARD
                     VRVCGHPLGGVPGEQHTPRQVDDGRVLLYLHGGAFVIGSCGSHRALASRLASAAHAQA
                     YVIDYRLAPEYPHPAQLQDALTAYRALLADGYDARNILLAGDSAGAALALMTLLAIRE
                     RKLPPPAALALISPWVDFTLSGATHRTHAQRDPLLDARWLVQALAWFSDGRPLPAPLL
                     EADYAGAPPTLIHVGSEEILLSDAENIAARLKGCGVAVELKRYDGLWHDFQLHAGVMA
                     EADAALDQMGAFLRQATAAGTARRAA"
     gene            complement(2347..4251)
                     /locus_tag="VHE37_14040"
     CDS             complement(2347..4251)
                     /locus_tag="VHE37_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVT36706.1"
                     /translation="MGKLINIDNGGTLTDFCVIDGDKVYRTKSVTTPYDLSKCFFDGL
                     RKASRLIYGEEDLQRLLASTDHIRYSTTQGTNALVERKGPRLGLVLGGALTAKALQAD
                     TAQKSLYDALVGARCETLDAKLEGDAAEAAAVRAVTQLASRGANRIVVAFGGAQRNAQ
                     EAALKRVLLRKFPPHMLGALPILYSHEVVEDNDDARRAWTALFNAFLHPAMERFLYSA
                     EHQLRGHKAQSPLLIFRNDGHSARIARTIAIKTYSSGPRGGAEGAKALAAHYGFPHLL
                     SMDIGGTTTDIALVAGNQVQLDRRGRVQGVATSFPLCNVESHGVGGSSIIKVEQGKIK
                     VGPESVGGAPGPACFGLGGKEATITDAFLLLGLLDPASYFGGELKIDLERARNAILEN
                     VGRPLKLSDEAAAQAMEKAWVEKVAATLTQFAKVTPDTTLAAFGGAGPFVVCRVAEAA
                     GIRHVIIPSLAAVFSAFGIGFSDIAHHYEAPLASNDDAALKQAKAALLERAQRGMFAE
                     GFELKDCRLDFTVQAGETSHALPGDSLPKGLSGALSLTLQATKPIAHTQLSGKFGGRR
                     LEAKAAGKRSTFFNGKRADAPLYRAEEQVAGATAQGPAVLEESFYTCRVDPGWSFEIN
                     AAGDILLSRA"
     gene            4505..5083
                     /locus_tag="VHE37_14045"
     CDS             4505..5083
                     /locus_tag="VHE37_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HVT36707.1"
                     /translation="MKTVVSALALAALAVTGAAQAEMMVRVRAIDILPDAKSSPVTGV
                     DVSNKVAPDVDISWLFSSHFATELLLTIPQSHKVTLNGTDIGRAAHLPPTLLLQYRVP
                     AMGAVEPYLGAGLNYTFFTDRKLNGGLELERGSFGPALQAGFDVKLSDGWYANVDVKK
                     IWIDTDVTSGGAFVTHVEINPLVIGIGLGRRF"
     gene            5189..5890
                     /locus_tag="VHE37_14050"
     CDS             5189..5890
                     /locus_tag="VHE37_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HVT36708.1"
                     /translation="MKNQPAVTQQQWLKARRALLAKEKKFTRLRDELARQRQALPWVR
                     VEKDYVFEGAKGRRTLAQLFDGRSQLVIYHFMFGPDWNAGCPHCSHWADSFDRAPVHL
                     AQRDVSFAAVSRAPYKKLAAYRKRMGWNFSWLSSAGSDFNFDFGASFTPQQLAEKKKV
                     YNFGTLAPGVSEREGVSVFCRDKRGRIFHTYSTFARGIDALSVDYQILDLTPKGRDED
                     GRGPYWVRRHDEYAS"
     gene            complement(6098..6838)
                     /locus_tag="VHE37_14055"
     CDS             complement(6098..6838)
                     /locus_tag="VHE37_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HVT36709.1"
                     /translation="MDQPALRSYRYYDLCLVGMVVVLLCSNLIGPGKVCAFNLPLYGV
                     LTFGAGNLFFPVSYIFDDVLTEVYGYARARRAIWAGFGAMTFATFMSWAIINMPADPN
                     EPYNKDLQPALEIALGNTARIAIASMLAYWLGDFANSYVLAKMKIWTNGRWLWTRTIG
                     STIVGEGLDSLTFYPIAFYGTWTGDTMARVIVFNWLLKVAVEVVFTPVTYWVVGKLKR
                     AEGLDTYDTHTDFNPFTLKDEGDVRRYQ"
     gene            complement(6944..7393)
                     /locus_tag="VHE37_14060"
     CDS             complement(6944..7393)
                     /locus_tag="VHE37_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVT36710.1"
                     /translation="MAPENRYEGGCQCGAVRYALNMAPQRSHYCHCRMCQRAVGGLFA
                     ALAGAKKEVFQWTKGTPGFFASSTLAQRGYCAACGTPLSFAYHEAPWIYVTIGSLDHP
                     SAAKPERHFGIESQLHWLEIDDDLPREATAVNDKLAAMTVFQFSTHG"
     gene            complement(7556..8989)
                     /locus_tag="VHE37_14065"
     CDS             complement(7556..8989)
                     /locus_tag="VHE37_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HVT36711.1"
                     /translation="MSANFIIAASFIAAAFLFIYGLKRMSSPVTARSGIVVAGYGMLV
                     AVLASFLYYFGVTEGAKPHLATNATLAAVALLLGVAWAWWSGKRVAMTAMPQMVALYN
                     GMGGGSAAAIAAMVLLAPAAGESEGGLLHYGVTVFGGLIGAVSLAGSLIAWAKLDGRI
                     NGTWRYTGLQAVNASVMLVTLGIGSTIVFAMHGHAPPMYTVGFFVMALVFGVFMTMPI
                     GGADMPVVISLYNAFTGLAVGFEGYVLQNPALMIAGMVVGSAGTLLTLLMAKAMNRSV
                     SNVIFSNFGATGDGAGEVTGTMKSAEAGDAAVQMKYASKVIIAPGYGLAVAQAQHKLY
                     EFVKLLEDRDVEVKFAIHPVAGRMPGHMNVLLAEAGVPYDIIYDMEDINGEFKEADVA
                     IVVGANDTVNPAARTNKSSPIYGMPILNVDQAKQVYVIKRGQGKGYSGVENELFFRDN
                     TSLVYGDAQKVMVAMIQALKSLEGGGH"
     gene            complement(8989..9303)
                     /locus_tag="VHE37_14070"
     CDS             complement(8989..9303)
                     /locus_tag="VHE37_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HVT36712.1"
                     /translation="MDATVTITGFVALYIFMLAAFTGYEIIGRVPSILHTPLMSGSNF
                     VHGIVVVGAIYATLNATSVLEQAIGFFGVLLGAGNAAGGYVVTERMLEMFKASDKKPG
                     AK"
     gene            complement(9306..>10101)
                     /locus_tag="VHE37_14075"
     CDS             complement(9306..>10101)
                     /locus_tag="VHE37_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HVT36713.1"
                     /translation="KITCFAMELVPRITRAQAMDALSSQAALAGYYGTLLGATQIARM
                     IPMTTFATGSIKPATILVMGVGVAGLSAIATARRIGARVEGYDVRPEVKEQVESLGAK
                     FIDTGVDARGQGGYARELTQEEKDKIAAVVTKHIQSADIIISTAAIPGRPSPKLISKA
                     QVDGMKAGAVIIDLAAEGGGNCEYSQPGETVQVGQVTINAPLNVPSMLAEHASELYSK
                     NQYNLIELMIKDKALALDWNDEVIAKTCLTHGGEIKNEAAKKAVGG"
BASE COUNT         1629 a         3325 c         3339 g         1808 t
ORIGIN      
        1 gccagcggtc gatcaccggg ttgaagtcgt agtgcttcac cccgccctgg tcgacgatgc
       61 cgatgtcgaa actggtgccg cccatgtcgg tggcgaccac gttgcccagc ccggtctgct
      121 gcatcaaatg ctcgctggcg gcgatgcccg acaccgggcc gctgtgcacg gtctgcagcg
      181 cgtcggtgga attgagctgc gccatgccgc cggagttgtg gttgaccagc atcggccgcg
      241 tgtagccggc ggcgcgcagg ttctgctcca gcgtgcccag gccgtgatac atggtctggt
      301 gcaggaaggc gtccatgatc gtcgacatgc cgcgcgcgta ctcgcccttg cgcccggcca
      361 cctggtgcga cagcagcatg gggatggcgc cgagcaggtg gcccggatat tcctcgagga
      421 atatctcgcg cacgcgcaac tcgtgtgcgt tgttgaccac gctgttggtg aacagcacca
      481 ccagcgcttc ggcgccggca tcgaccagct cgcggatctt cagccgcacg tcatcctcgt
      541 tgagcgtgag gaacaccttg cccaggtagt ccacacgctc gcggatctcg cggatcatcg
      601 ggatcggcac gatcggttcc ggccgccgcg cgttgggaat gtccatctgc cgctcttccg
      661 gcaggccttc gccgtagccg cgcgcgcgcg acagcgggat ggtgctcttg aagccggcgg
      721 tgaccagcac gccgatgcgc ggacccttgc gctcgatcag cgcgttggtg cccagcgtgg
      781 tggcgtagcg caccgagtcc acctgcgaca gcagcgtgtt cacttcgagc ttgaggtcct
      841 tgcaggcatt gcccagcgcc tcgttgaagc ccagcgccag gttgtgatgc gtggtcagcg
      901 ccttgccctc gatgtactgg ttgtcccaga ccacgaagca gtcggtgaag gtgccgccga
      961 tgtccacgga gacgcgtttc atggtgcgct cctgtcagtg cttgtgacca tgactgtgct
     1021 tgtgctccag ccgcggcggc cggatgtacg ggccgggcgt cacgtccttc acttccggct
     1081 tgtccttcca ttgcagcttc agcgcatcga tgtcgagctc aatgtcgtgc agcggcggat
     1141 ggcccggcgg caggtactcg gcctcgcaca tggttccgca ttgcgggcag tagtactcga
     1201 ggatgcggca ccaggtcggg tcgggcgagt aggtgcgctc gtacttcttc gtgtcgagca
     1261 gcggcttgtg gatctcgcgc ggatcgcggt cgtagaccag caggccgcgc ttgtagttgc
     1321 cgcgcgcgct gcccagttcc ttgccgcagc ggcggcattc ccattgttcg ttgtcgagat
     1381 tgatgcgcag gtattcggtg atcagcactc tcatcgccac tctcctcgtg ttctcttatg
     1441 cggcgcggcg cgcggtaccg gccgccgtcg cctgccgcag gaatgcgccc atctggtcca
     1501 gcgcggcgtc ggcttccgcc atcacgccgg catgcagctg gaagtcgtgc cacaggccgt
     1561 cgtagcgctt gagctccacc gcgacgccgc agcccttcag ccgtgcggcg atgttctccg
     1621 cgtcgctcag caggatttcc tcgctgccga catggatcag cgtgggcggc gcgccggcgt
     1681 agtcggcttc gagcagcggc gccggcaggg gccgtccatc ggaaaaccac gcgagcgcct
     1741 gcaccagcca gcgtgcgtcg agcagcggat cgcgctgcgc gtgcgtgcga tgggttgcac
     1801 cggacagggt gaaatccacc cacggtgaaa tcagcgcgag cgcggcgggc ggcggcagct
     1861 tgcgttcacg gatggccagc agcgtcatca gcgccagcgc cgcgccggcg gaatcgccgg
     1921 ccagcaggat gttgcgcgcg tcatagccgt cggcgagcag ggcgcggtaa gcggtcagcg
     1981 cgtcctgcag ctgcgcggga tgcggatatt ccggcgccag ccggtagtcg atgacgtagg
     2041 cctgtgcgtg cgcggccgac gccagccgcg aagccagcgc gcggtgcgag ccgcaggaac
     2101 cgataacgaa cgcgccgccg tgcaggtaaa gcagcacgcg gccgtcatcg acctggcgtg
     2161 gagtgtgctg ctcgcccggc actccgccga gcgggtggcc gcacacgcgc acgtcgcgcg
     2221 ccacgcgcga gttgagccac aggcccgccg cggccaccgc gcgctgcagc cacagcggaa
     2281 acggtggccg caacaggggc ttcagtcccc agcgcgccat gaacttgtgg gtggcggcgc
     2341 gcatgctcag gcgcggctca gcaggatgtc gcccgccgca ttgatctcga agctccaccc
     2401 gggatcgacg cggcaggtgt agaagctttc ctccagcacc gccgggccct gcgcggtggc
     2461 gccggcaacc tgctcttcgg cgcggtacag cggcgcatcg gcgcgcttgc cgttgaagaa
     2521 ggtgctgcgc ttgcccgctg ccttggcctc caacctgcgt ccgccaaact tgccggagag
     2581 ttgcgtgtgc gcgatgggct tggtcgcctg cagcgtgagc gacagcgcgc cggagagccc
     2641 cttgggcaga ctgtcgcctg gcaacgcgtg cgacgtctcg ccggcctgca cagtgaaatc
     2701 gagcctgcag tccttcaatt cgaaaccttc ggcgaacatg ccgcgctgtg cgcgttcgag
     2761 caacgcggcc ttggcctgct tcagcgcggc gtcatcgttg gacgccagcg gcgcttcgta
     2821 gtgatgcgcg atgtcggaga aaccgatgcc gaaggccgag aacaccgccg ccaggctcgg
     2881 gatgatcacg tgccggatgc cggccgcttc ggctacgcgg cacaccacga acggcccggc
     2941 gccgccgaag gccgcgagcg tggtgtcggg tgtgaccttg gcgaactgcg tcagcgtggc
     3001 cgcgaccttc tcgacccagg ctttttccat cgcctgcgcc gccgcttcgt ccgacagctt
     3061 gagcggacgc cccacatttt ccaggatggc gttgcgtgcg cgctccaggt ctatcttcag
     3121 ctcgccgccg aaatagctgg ccgggtcgag caggccgagc agcaggaagg cgtcggtgat
     3181 ggtcgcctcc ttgccgccga ggccgaagca ggccgggccg ggcgcaccgc cgaccgactc
     3241 cgggccgact tttatttttc cctgttcgac cttgatgatc gaactgccgc ccacgccgtg
     3301 gctctccacg ttgcacagcg ggaacgaggt ggccacgccc tgcaccctgc cgcggcggtc
     3361 gagttgcacc tggttgccgg cgaccagcgc gatgtcggtg gtggtgccgc cgatgtccat
     3421 cgacagcagg tgcggaaagc cgtagtgcgc ggccagcgcc ttggcgcctt ccgcgccgcc
     3481 gcgcgggccc gacgaatagg tcttgatggc gatggtgcgg gcgatgcgcg ccgagtggcc
     3541 gtcgttgcgg aagatcagca gcgggctctg cgccttgtgc ccgcgcagct ggtgttcggc
     3601 ggaatagagg aagcgctcca tcgccggatg caggaaggca ttgaacagcg cggtccacgc
     3661 gcggcgtgcg tcgtcgttgt cctcgaccac ttcgtgcgag tacaggatcg gcagtgcgcc
     3721 gagcatgtgc ggcgggaatt tccgcagcag cacgcgcttg agcgccgctt cctgcgcgtt
     3781 gcgctgcgcg ccgccgaagg caaccacgat gcggttggcg ccgcgggagg ccagctgcgt
     3841 taccgcgcgc actgctgccg cctcggcggc atcgccttcc agttttgcgt cgagcgtctc
     3901 gcagcgcgca ccgaccagcg cgtcatacag actcttctgc gcggtgtcgg cctgcagcgc
     3961 tttcgccgtc agcgcaccgc cgagcaccag tcccagccgc ggccccttgc gctcgaccag
     4021 cgcgttggtg ccctgcgtgg tggagtagcg gatgtggtcg gttgaggcga gcaggcgctg
     4081 caggtcttcc tcgccataga tcagccggct ggccttgcgc agtccgtcga agaagcactt
     4141 cgacaggtca tagggcgtgg tcaccgactt ggtgcggtag accttgtcgc cgtcgatcac
     4201 gcagaagtcg gtgagcgtgc cgccgttatc gatgttgatc agctttccca tgaggcgtcg
     4261 tcctctcgtg cacgcgcggc gcggaaccga accactcggc cgcgccggaa tgtgggaatt
     4321 tttacgccgg gcaaaaacgg ctttcgctac ccgctcgggt agtcttgtgc gcgacagcgc
     4381 gccgcgctcc atcgtcgggc gatggctgac tcgcacgtca atgacctggg tcaactcatt
     4441 tgccgcgccc gcctcctatt gtgcgttgcc cgcattttcg ggccattaac cgtggagtcg
     4501 cgagatgaaa accgtagtga gtgcgctggc acttgcggcc ctggccgtca ccggcgccgc
     4561 ccaggccgag atgatggtgc gcgtgcgcgc catcgacatc ctgcccgacg ccaagtcgag
     4621 cccggtgacc ggcgtggacg tatccaacaa ggtggcgccc gatgtcgaca tcagctggct
     4681 gttcagttcg cacttcgcca ccgagctgct gctgaccatt ccgcagtcgc acaaggtgac
     4741 gctcaacggc accgacatcg gcagggccgc acacctgccg ccgacgctgc tgctgcagta
     4801 ccgggtgccg gcaatgggcg ctgtcgagcc gtacctcggc gccggcctca actacacctt
     4861 cttcaccgac cgcaagctca acggcgggct ggagctcgag cgcggcagct tcggcccggc
     4921 gctgcaggcg ggcttcgacg tgaagctgag tgatggctgg tatgccaacg tcgatgtgaa
     4981 gaagatctgg atcgacaccg acgtcacttc gggcggcgcc ttcgtcaccc acgtggagat
     5041 caatccgctg gtgatcggca tcggcctggg ccggcgtttc tagggccgcg ctgcatccgc
     5101 acgcccgccg ccggaccggc gggcgccgcg gccgtcacct gcgcccgcct cgaacgcgct
     5161 ataacacgcg cggcgagcgg aggcgcagat gaagaaccag ccggcggtga cgcagcagca
     5221 atggctcaag gcgcggcgcg cactgctggc gaaggaaaag aaattcaccc gcctgcgcga
     5281 cgaactcgcg cgccagcgcc aggcgctgcc gtgggtgcgc gtggaaaagg actacgtgtt
     5341 cgaaggcgcc aagggccggc gcacgctggc gcagctgttc gacggacgca gccagctggt
     5401 gatctaccac ttcatgttcg ggccggactg gaacgccggc tgcccgcatt gctcgcactg
     5461 ggccgacagc ttcgatcgcg cgccggtgca cctggcgcag cgcgatgtca gcttcgctgc
     5521 cgtgtcgcgc gcgccgtaca aaaagctggc ggcctatcgc aagcgcatgg gctggaattt
     5581 ttcctggctg tcgtccgccg gcagcgactt caacttcgat ttcggtgcct cgttcacgcc
     5641 gcagcagctt gcggaaaaga agaaggtcta caacttcggc acgctggcgc cgggcgtttc
     5701 cgaacgcgaa ggcgtcagcg tgttctgccg cgacaagcgc gggcgcatct tccacaccta
     5761 ctcaacgttt gcccgcggca tcgatgcgct gagcgtggac taccagatcc tcgacctcac
     5821 gcccaagggc cgcgacgagg acgggcgcgg gccatactgg gtgcgcaggc acgacgaata
     5881 cgcgtcgtaa aaccctgtac cgcaccggcc tcgccactgt tataaaaact tgccttattt
     5941 tattttcaaa caataactac taacagccgc aaaggcggtg cggtcaggcc atttttcgtg
     6001 cgccgcggag tcctgcgcga ggcgtgtcaa ctcttcacgc gcagccgtcc gccggtgggg
     6061 cggaagatgc ggcgcatcat ggaacttgct tcttgattta ctggtagcgg cgcacgtcgc
     6121 cttcatcctt cagcgtgaag ggattgaagt cggtgtgcgt gtcgtaggtg tccagcccct
     6181 cggcgcgctt gagcttgccg accacccagt aggtcaccgg cgtgaacacc acctccaccg
     6241 ccaccttgag cagccagttg aacacgatga cgcgcgccat ggtgtcgccg gtccaggtgc
     6301 cgtagaacgc gatcggatag aacgtcagcg agtccagccc ttcgccgacg atggtcgagc
     6361 cgatggtgcg cgtccacagc cagcggccat tggtccagat cttcatcttg gccagcacat
     6421 aactgttggc gaaatcgccg agccagtagg ccagcatcga ggcgatggcg atgcgcgcgg
     6481 tgttgcccaa ggctatctcc agcgcgggct gcaggtcctt gttgtacggc tcgttcggat
     6541 cggccggcat gttgatgatg gcccacgaca tgaaggtggc gaaggtcatg gcgccgaagc
     6601 cggcccagat ggcgcggcgc gcgcgcgcgt agccgtacac ctcggtcagc acgtcgtcga
     6661 agatgtacga caccgggaag aacagattgc cggcgccgaa ggtgagcacg ccgtacagcg
     6721 gcaggttgaa cgcgcagacc ttgccggggc cgatcaggtt ggagcacagc agcaccacca
     6781 ccatgccgac caggcacagg tcgtagtagc ggtagctgcg cagcgccggc tgatccatcg
     6841 atagccccgt cattcccgcg aaggcgggaa tccagctttg attttagtgg ctgcccgcga
     6901 caaaaaactg gattcccgcc ttcgcggaat gacgaatgcg gtttcaaccg tgcgtggaaa
     6961 actggaacac cgtcatcgcc gccagcttgt cgttgaccgc ggtcgcttcg cgcgggaggt
     7021 catcgtcgat ctccagccag tgcagctggc tctcgatgcc gaaatgccgt tcgggtttgg
     7081 ccgcggaggg atggtcgagc gaaccgatgg tcacgtaaat ccacggcgcc tcgtggtacg
     7141 cgaacgacag cggcgtgccg caggccgcgc agtagccgcg ctgcgccagc gtggacgagg
     7201 cgaagaagcc cggcgttccc ttcgtccact ggaagacttc cttcttcgcg ccggcgagcg
     7261 ccgcgaacaa accgccgacc gcgcgctggc acatgcggca atgacagtag tgcgagcgct
     7321 gcggcgccat gttcagcgcg tagcgcaccg cgccgcactg gcagccgcct tcgtagcgat
     7381 tttcaggggc catttcgtct cccggacatc taaccgcacc gcctgttttg atgttattaa
     7441 ttattgtttt attattaaat tatacaatct ttaataacat tctgttgcgc gatgcggtgg
     7501 gcctattctg gcaacaaaaa agccgcgcga ggcggctttt ttcactgcgc gcaactcagt
     7561 gtccgccgcc ttcgagcgac ttcagcgcct ggatcatcgc caccatcacc ttctgcgcat
     7621 cgccgtagac caggctggtg ttgtcgcgga agaacagctc gttctccacg ccggagtaac
     7681 ccttgccctg gccgcgcttg atcacgtaga cctgcttggc ctggtcgacg ttgaggatcg
     7741 gcatgccgta gatcggcgaa gacttgttgg tgcgcgcggc cgggttcacc gtgtcgttgg
     7801 cgccgaccac gatcgcgaca tcggcttcct tgaactcgcc gttgatgtct tccatgtcgt
     7861 agatgatgtc gtacggcacg ccggcctcgg ccagcagcac gttcatgtgg ccgggcatgc
     7921 ggcccgccac cggatggatg gcgaatttga cctcgacatc gcgatcctcc agcagcttca
     7981 cgaactcgta gagcttgtgc tgcgcctgcg ccaccgcgag gccgtagccc ggcgcgatga
     8041 tgaccttgct cgcgtacttc atctgcaccg cggcgtcgcc ggcctcggcc gacttcatgg
     8101 tgccggtgac ttcgcccgcg ccgtcgccgg ttgcgccgaa gttgctgaag atgacgttcg
     8161 acacgctgcg gttcatggcc ttggccatga gcagcgtcag caacgtaccc gccgagccga
     8221 ccaccatgcc ggcgatcatc agcgccgggt tctgcagcac gtaaccttcg aagcccacgg
     8281 ccaggccggt gaatgcgttg tacagcgaga tcaccaccgg catgtcggcg ccgccgatgg
     8341 gcatggtcat gaacacgccg aacaccagcg ccatgacgaa gaagccgacg gtgtacatgg
     8401 gcggcgcgtg tccatgcatc gcgaatacga tggtgctgcc gatgccgagc gtcaccagca
     8461 tgacgctggc gttcaccgcc tgcaggccgg tgtagcgcca ggtgccgttg atgcggccgt
     8521 cgagcttggc ccaggcgatc agcgagccgg ccagcgagac cgcgccgatc aggccgccga
     8581 acaccgtcac gccgtagtgc agcaggccgc cttcggattc acccgctgct ggcgcgagca
     8641 gtaccatcgc cgcgatcgcc gccgcggagc cgccgcccat gccgttgtac agcgccacca
     8701 tctgcggcat cgccgtcatg gccacgcgct tgccgctcca ccaggcccag gccacgccga
     8761 gcagcagtgc gacggcggcg agcgtggcgt tggtcgccag gtgcggcttg gcgccttcgg
     8821 tgacgccgaa gtaatagagg aagctggcga gcaccgcgac cagcatgccg tagccggcca
     8881 cgacaattcc cgagcgcgcc gtcaccgggg aactcatgcg cttcaagcca tagatgaaca
     8941 ggaaagcggc ggcgatgaaa ctcgcggcga tgatgaagtt ggcgctcatc acttggctcc
     9001 cggcttcttg tcggaggcct tgaacatttc cagcatgcgc tcggtgacca cgtaaccgcc
     9061 ggcggcgttg ccggccccga gcagcacgcc gaagaagccg atggcctgct ccagcaccga
     9121 ggtggcgttg agcgtggcgt agatcgcgcc gaccacgacg atgccgtgca cgaagttgct
     9181 gcccgacatc agcggcgtgt gcaggatcga cggtacgcgg ccgatgatct cgtagccggt
     9241 gaaggcggcc agcatgaaga tgtacagggc gacgaagccg gtgatggtga cggtggcgtc
     9301 catgtttatc ccccaaccgc tttcttggcg gcctcgttct tgatctcgcc gccgtgcgtc
     9361 aggcaggtct tggcgatcac ctcgtcgttc cagtccagcg ccagcgcctt gtccttgatc
     9421 atcagctcga tcaggttgta ctggttcttc gagtacagct cgctggcgtg ctcggccagc
     9481 atcgacggca cgttgagcgg cgcgttgatg gtgacctggc ccacctgcac ggtctcgccc
     9541 ggctggctgt actcgcagtt gccgccgcct tccgcggcga ggtcgatgat caccgcgccg
     9601 gccttcatgc cgtcgacctg cgccttgctg atcagcttgg gcgagggccg gccgggaatg
     9661 gcggcggtgg agatgatgat gtcggccgac tggatgtgct tggtcaccac cgcggcgatc
     9721 ttgtccttct cttcctgggt cagctcacgt gcatagccgc cctgcccgcg cgcgtcgacg
     9781 ccggtgtcga tgaacttggc gccgagcgac tcgacctgct ccttcacctc gggacgcacg
     9841 tcgtagcctt ccacgcgtgc gccgatgcgg cgcgcggtgg cgatggcgga aagaccggcg
     9901 acgcccacgc ccatcaccag gatggtggcc ggcttgatcg agccggtggc gaaggtggtc
     9961 atcgggatca tgcgcgcgat ttgcgtcgct cccagcagcg tgccgtagta gccggccagt
    10021 gccgcctgcg agctcaatgc gtccatcgcc tgcgcgcgcg tgatgcgcgg caccagctcc
    10081 atggcgaagc aggtgatctt g
//
LOCUS       DASZZC010000663         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1269530,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000663 DASZZC010000000
VERSION     DASZZC010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269530"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VHE37_14080"
     CDS             <1..1196
                     /locus_tag="VHE37_14080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HVT36714.1"
                     /translation="PAARTRPLENTLAITGKNEGFTATHTLDEPLTLPPASAGAPAST
                     MATGAKPSERVDFDVTSQFAESTMSINLDANDPVSEADFHLAYGLYDEAALLLKQAAE
                     KDPKRTELRVKLAETYFAASRPQEFLEVAGTLKNEMPTAEWQKLAIMGRQLCPDAALF
                     KGADESAALGGDVDLSFDEPSAPATPAPAPRAAAPAPKADAGLDFKLEELELSQLEPT
                     RLVPRETVIPSTPPPPPPPPDDDGALDFKLEDIELKPSTEPGSELSAADMGHSIDFNA
                     TIAEPAATPAPLPDPLARTGGFSSKTAETPSFEEIATATAVMTDGPSSKKRSDEVSLD
                     DFDLGDDSAAISSGDEAGTKLDLARAYADMGDNDMAKTLLKEVVESGNAEQKKEAQAL
                     IGRLG"
     gene            1216..>1846
                     /gene="truA"
                     /locus_tag="VHE37_14085"
     CDS             1216..>1846
                     /gene="truA"
                     /locus_tag="VHE37_14085"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVT36715.1"
                     /translation="MSGEQRWAAGVEYLGTHYAGWQRLSGIQSVQEKIERALSRVADH
                     PVNIIAAGRTDAGVHALQQVIHFDSPSRRTPYAWLLGTNSNLPHDISLRWVQPVSGDF
                     HARYGARARSYRYVILNQRARAALLHERTAFWPMPLDAAAMHGAAQVLVGEHDFSAFR
                     DSQCQSPTAMREVKSIELARRGDFVVLDICGNAFLHHMVRNIAGTLLEVG"
BASE COUNT          332 a          635 c          612 g          267 t
ORIGIN      
        1 aaccggcggc gcgcacacgc ccgctggaaa acacgctggc catcaccgga aagaacgaag
       61 gtttcaccgc cacgcacacg ctggacgagc cgctgacgct gccgccggct tctgccggcg
      121 ccccggcctc caccatggcc acgggcgcca agccgtccga gcgcgtggac ttcgacgtca
      181 cttcgcagtt cgccgaatcg accatgtcga tcaacctcga cgccaatgat ccggtgtccg
      241 aggcggattt ccacctggcc tacggcctgt acgatgaggc tgcgctgctg ctcaagcagg
      301 cggcggagaa ggatcccaag cgcaccgaac tgcgcgtaaa gctggcggaa acctatttcg
      361 ccgccagccg tccgcaggaa ttcctcgagg tggccggcac gctcaagaac gagatgccga
      421 ccgccgagtg gcagaagctg gccatcatgg ggcggcagct gtgcccggat gcggcgctgt
      481 tcaagggtgc ggacgaatcg gccgcgctcg gcggagacgt cgacctgtcg ttcgacgagc
      541 cgtccgcgcc tgcaactccc gcccccgcgc cgcgcgcggc agcgcctgcg ccgaaggctg
      601 atgccggcct ggacttcaag ctcgaggaac tggagctgtc gcagctggaa cccacccgcc
      661 tggtgccgcg cgagacggtg atcccgtcga cgcctccgcc tccgccgcct ccgcccgatg
      721 atgacggcgc gctggacttc aagctcgaag acatcgagct gaagccttcg accgagccgg
      781 gctcggaact cagcgcggcg gacatgggtc acagcatcga cttcaacgcc accatcgccg
      841 aaccggccgc gacgccggcg cctttgcccg atccgctggc gcgtaccggc ggcttcagtt
      901 ccaagacggc ggagacgccg tcgttcgagg agatcgccac ggcaacggcc gtgatgaccg
      961 acggcccgtc gagcaagaag cgcagcgacg aagtaagcct ggacgacttc gatctcggcg
     1021 acgattcggc ggccatcagc agcggcgacg aggcgggcac caagctcgac ctggcgcgcg
     1081 catatgccga catgggcgac aacgacatgg cgaagacgct gctcaaggaa gtggtggaga
     1141 gcggcaacgc cgagcagaag aaggaagcgc aggcgctgat cggccgcctc ggctgaagcg
     1201 gcgcgcgcac gtgccgtgag cggcgagcag cgctgggccg ccggcgtcga atacctcggc
     1261 acgcactacg ctggctggca gaggctgagc ggcatccagt cagtgcagga aaagatcgag
     1321 cgcgcgctgt cgcgcgtcgc cgatcatccg gtgaatatca ttgccgccgg gcgcaccgat
     1381 gccggcgtgc atgcgctgca gcaggtgatc cacttcgaca gcccctcgcg gcgcacgcca
     1441 tatgcatggc tgctcggcac caattccaac ctgccgcacg acatcagcct gcgctgggtg
     1501 cagccggtga gcggtgattt ccatgcgcgc tacggcgcgc gggcgcgcag ttaccgctac
     1561 gtgatcctca accaacgcgc gcgcgccgca ctgctgcacg agcgcaccgc gttctggcct
     1621 atgccgctcg atgccgcggc gatgcacggg gccgcgcagg tgctggtggg cgagcatgat
     1681 ttttccgcct tccgcgattc gcaatgccag tcgcccacgg cgatgcgcga ggtgaaatcg
     1741 atcgagctcg cacgccgcgg cgacttcgtg gtgctcgata tttgcgggaa tgcttttctg
     1801 caccacatgg tgcgcaatat cgccggcacg ctgctcgaag tcggcc
//
LOCUS       DASZZC010000664         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1021225,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000664 DASZZC010000000
VERSION     DASZZC010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021225"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VHE37_14090"
     CDS             <1..246
                     /locus_tag="VHE37_14090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HVT36716.1"
                     /translation="GGLKEKLLAAQRGGITTVVIPQENVKDLADIPDNVKSKLNIEPV
                     RWIEDVLKFALERMPEPRPDEPAPPAAAPAGEGARAH"
     gene            351..1034
                     /locus_tag="VHE37_14095"
     CDS             351..1034
                     /locus_tag="VHE37_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HVT36717.1"
                     /translation="MPTVRDYRRANLASLIAQAGTQARLADQTGLARAFVSQVMIRNR
                     ELGDRAARQIERRMGLPHGALDLPAMTSPVMEVREPAAHYGDAAAPARIAQACGARAG
                     QGKTRKVEWLTDPVAATPWKQELPAQLGLRAERCISLTAVSDNMEPAIRKGDQLLIDI
                     SSGTVRDGGIHVVVTGDRIELLRLRRQRGGMILAQSDHPDQSRYPPRLLDAARSAIAG
                     RVVWRQGAL"
     gene            1185..1928
                     /gene="dksA"
                     /locus_tag="VHE37_14100"
     CDS             1185..1928
                     /gene="dksA"
                     /locus_tag="VHE37_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HVT36718.1"
                     /translation="MKSKKPAARPAAKAANAKSKKPAAKHVPKAKPLAAKKPAKASKP
                     AAAPKKHSAVKAAASKLRGLFTKPASNKKSPDQPASVVSNRTPARPTAPSKMQTATKP
                     FQSKLVPPKGLLSEAQIRRMSDKDYMNDSQLEFFRHRLFQMREEVLAREVDVKERLHQ
                     REVFADPADRATAEEEHWLDLRLRERESLLLRKIDEALRRIAEKEFGYCEKTGDPIGI
                     PRLLARPTATVCVDVKGQDERVEAQFRDR"
     gene            2018..2094
                     /locus_tag="VHE37_14105"
     tRNA            2018..2094
                     /locus_tag="VHE37_14105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2052..2054,aa:Arg,seq:tct)
     gene            2265..3074
                     /locus_tag="VHE37_14110"
     CDS             2265..3074
                     /locus_tag="VHE37_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT36719.1"
                     /translation="MSTQDQVFAGSIPSIYQTALVPLIFQHYADDLARRLAGHPVSRL
                     LEIAAGTGVVTRVLAATLPGSAAIVATDLNPAMIEEARRVDTPRPVEWRTADAMHLPF
                     DDGSFDAVVCQFGAMFFPDRAAAFAQARRVLRPGGLFLFSVWDRIADNHFADTVTTAL
                     GQRYPGDPPRFLARTPHGYHDRAVVGRDVRAAGFATVQIDTVSASSKAVSADIVAMAY
                     CQGTPLRGEIEAREPSGLAAATETARAALAQRFGHGTIEGKIQALVVTAAD"
     gene            complement(3085..>3718)
                     /locus_tag="VHE37_14115"
     CDS             complement(3085..>3718)
                     /locus_tag="VHE37_14115"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT36720.1"
                     /translation="LQQAIKGAKLIEIEGGDSFFSHDRAEEVLGYIEEFLTGTRAQTY
                     AERVLATVLFVDIAGSTRLAAKIGDSAWRDLAARFHAMVRTQLHRFRGREIDAAGDGA
                     FATFDSPTQAIRAAAAIRAEALTLGVSVRAGMHTGECEVADGVVRGLAVHIGARVMGE
                     AERDEILVSNTVKDLVAGSDFEFKDRGKHQLKGVPGVWRLHALDRIEAGR"
BASE COUNT          663 a         1285 c         1182 g          588 t
ORIGIN      
        1 ggcgggctga aggaaaagct gctggcggcc cagcgcggcg gcatcaccac ggtcgtcatt
       61 ccacaggaaa acgtgaaaga cctggccgac attccggaca acgtgaagag caagctcaac
      121 atcgaaccgg tgcgctggat cgaggatgtg ctgaagttcg cgctggagcg catgccggaa
      181 ccgcgtccgg acgagccggc gccgcctgca gcagcgccag ccggcgaagg agcccgcgct
      241 cactaggtca gcgctttgcg tggatggccc gcagatcccg cggggcattc cggcttgaag
      301 ttggcccgcg catcgttggg agcgatattg aattcctgcc tggctgacgc atgcccacag
      361 tccgcgacta ccggcgcgca aatctcgcca gcctgattgc gcaggcggga acccaggcca
      421 ggctggcgga ccagaccggc ctggcgcgcg ccttcgtcag ccaggtgatg atccgcaacc
      481 gggaactggg cgatcgggcg gcgcggcaga ttgagcgccg aatgggtctg ccccatgggg
      541 cgctggacct tcccgcgatg acatcgccgg tcatggaagt ccgcgagccc gctgcgcact
      601 acggcgatgc cgccgctccg gcgcggatcg ctcaagcctg tggggcacgc gccggccagg
      661 gaaagacccg caaggtggaa tggctgaccg atcccgttgc ggctacaccg tggaagcagg
      721 aattgccggc ccagcttgga ctgcgagcgg agcgctgcat cagcctgact gcagtcagcg
      781 acaacatgga acccgccatc cgcaagggtg accagctgct gatcgatatt tcatcgggga
      841 cggtccgcga cggcggcatc catgtcgtgg tcaccggcga ccgcattgaa ttgctgcggc
      901 tcaggcgcca gcgcggcggc atgatcctgg cgcagtcgga ccaccctgat caatcccggt
      961 atcccccccg cctgctcgat gcggcgcgca gcgccatcgc cggccgcgtg gtctggcggc
     1021 agggagcgct ttgaaccggc gccgcctggt ccgctcccgc gacggcagct gaccggctcg
     1081 tccgcgagag gccgtttgac gctacccagg cccggtgcta taacgcgcga ctttcgcgat
     1141 tcccctcgcg gaatcaggca ctttcaacag agcgaaaacc agcgatgaag tcgaaaaaac
     1201 cggcggcgcg tccagccgcc aaggccgcga acgccaagag caagaagccg gcggccaagc
     1261 acgtgcccaa agccaagccg ctcgcggcga agaagccggc caaggcctcg aaacctgccg
     1321 ctgctccgaa aaagcattcc gctgtaaagg cagctgcctc taagcttcgc gggctcttca
     1381 caaagcctgc gtccaataaa aaaagtcccg atcaaccggc ctctgtggtg tccaacagga
     1441 ctccggcacg tcccactgcc ccatcaaaga tgcaaaccgc tacgaaacct ttccagtcga
     1501 aattggtgcc gcccaagggc ctgctatccg aagcccagat acgccgtatg tccgacaagg
     1561 actatatgaa cgactcgcag cttgagttct tccgccatcg cctgttccag atgcgcgaag
     1621 aagtgctggc gcgcgaggtg gatgtgaagg aacgcctgca tcagcgcgag gtgtttgccg
     1681 atccagccga tcgcgccacc gcggaagagg agcattggct ggacctgcgc ctgcgcgaac
     1741 gcgaatcgct gctgctgcgc aagatcgacg aagccctgcg ccgcattgcc gagaaggaat
     1801 tcggctattg cgagaagacc ggcgacccga tcggcatccc gcgcctgctg gcccgcccca
     1861 ccgccaccgt ctgcgtggac gtgaaaggcc aggacgagcg cgtcgaagcg caattccgcg
     1921 accgctgaag cttcacgaga ttcaccgcag ccggccccgt gccggctgtt ttcgtttgag
     1981 gcgaggcgat gaacaggtgc actacacttc gcagcaagcg cccgtagctc agttggatag
     2041 agcattgacc ttctaagtca agggtcgcag gttcgactcc tgccgggcgc gccattctca
     2101 atgcgagatc ttatgcagga gtctcacctg ccaccgcggt cgcaggttgc gcggtgcatc
     2161 catgcaccgc ggccttcggc ctcgctacgc tcgctcgatt tcgctttcct gcgaaatcgt
     2221 cgactcctgc cgggcgcgcc cttctcgaaa ttccaggagg cttcatgagc acccaggacc
     2281 aggtattcgc cggctccatc ccgtcgatct accagaccgc gctcgttccc ctgatcttcc
     2341 agcattacgc ggacgacctg gcccggaggc tcgcggggca cccggtttca cgcctgctgg
     2401 aaatcgcagc cggcaccggc gtcgtcaccc gcgtacttgc cgcgaccttg cccggcagcg
     2461 cagcgatcgt cgccaccgac ctcaaccccg ccatgatcga ggaagcgcgc cgcgtggaca
     2521 caccgcggcc ggtggaatgg cgcacggccg atgccatgca cctgcccttc gacgacggca
     2581 gcttcgacgc cgtcgtctgc cagttcggcg cgatgttctt ccccgaccgc gccgcggcgt
     2641 tcgcccaggc gcgccgcgtg ctgaggccgg gcgggttgtt cctgttcagc gtgtgggacc
     2701 gcattgccga caatcatttt gccgacacgg tgacgacggc gctgggccag cgttacccag
     2761 gcgatccgcc acgcttcctc gcgcgcacgc ctcacggcta tcacgaccgc gccgtcgtcg
     2821 ggcgcgacgt gcgcgcggcg ggcttcgcca ccgtgcagat cgacaccgtc agcgccagct
     2881 cgaaagccgt ctcggcggat atcgtggcga tggcctattg ccagggcacg cccctacgcg
     2941 gcgaaatcga ggcgcgcgag ccgtcgggcc tggccgccgc caccgagact gcgcgcgcag
     3001 cccttgcgca gagattcgga catggaacga tagagggaaa aatccaggcg ctggtggtga
     3061 cggccgccga ctagccgttc gcgctcaacg cccggcttcg atgcggtcca gcgcgtgcag
     3121 gcgccacacg cccggcacgc ccttcagctg atgcttgccg cgatccttga attcaaaatc
     3181 ggagccggcg accaggtcct tgaccgtgtt cgacaccagg atttcgtcgc gctctgcctc
     3241 gcccatcacg cgcgcgccga tatgcacggc cagtccgcgt actacgccgt cggccacctc
     3301 gcactcaccc gtgtgcatgc ctgcgcgcac gctcacaccc agcgtcagcg cctcggcgcg
     3361 gatggccgcg gcggcgcgta ttgcctgcgt cggcgaatcg aaggtggcaa acgcgccatc
     3421 gccggcggcg tcgatctcgc gcccgcggaa ccggtgcagc tgcgtgcgca ccatggcgtg
     3481 gaaacgcgcg gccaggtcgc gccaggcgga gtcgccgatc ttggccgcca gccgcgtgga
     3541 cccggcgatg tcgacgaaca gcacggtggc cagcacgcgc tcggcatagg tctgcgcgcg
     3601 cgtgccggtg aggaattcct cgatgtagcc gagcacttcc tcggcgcggt cgtgcgagaa
     3661 gaatgaatcg ccgccttcga tctcgatcag cttcgcgccc ttgatcgcct gctgcagg
//
LOCUS       DASZZC010000665         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_248407,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000665 DASZZC010000000
VERSION     DASZZC010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248407"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VHE37_14120"
     CDS             <1..823
                     /locus_tag="VHE37_14120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT36721.1"
                     /translation="AAPKSVDPVKAEKRLREALAQIAQVLQIPAARIHYKLRKSQKGT
                     AQYAKQDESGVFHIVAEHGCKLWVNFDDYLDTGVFLDHRPLRLRIQQEARGRKFLNLF
                     AYTGSASVHAAVGGASSTVSVDLSNTYLEWAQRNLELNGFHARDEERRSSLENAHRLL
                     RADCREWLAEQSVKSRPPLFDLILCDPPTFSNSKRMEGTLDTQRDHVDLIRHCEKLLA
                     PGGVLYFSTNRRGFKLDSEALAGLEIKDITAQTLDEDFKRPPPAHKCWRIARGAQ"
     gene            complement(876..1568)
                     /locus_tag="VHE37_14125"
     CDS             complement(876..1568)
                     /locus_tag="VHE37_14125"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HVT36722.1"
                     /translation="MQHPSLYYRRMAVAGMLFAGAAAVPTARADDTVPPVQYSGRATV
                     VDATVKVLTANAHVVLGDTGYLDPTGGTREDTVAQIDNPPPLGLHSSTIDAITSGANG
                     TASSHAEVEKLAVTISALKITADVIEADTTAVCDQSSQTVAPSGSSTIVNLKINGKGY
                     AYSGKPNQKISIPGVATIILNEQSQPDVNSYSVNAIHVIVPGAPGVASANVIISHAES
                     GIVSCPCNTPVP"
     gene            complement(1659..>2200)
                     /locus_tag="VHE37_14130"
     CDS             complement(1659..>2200)
                     /locus_tag="VHE37_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36723.1"
                     /translation="SYVVGIPALTGAASGDLLRVNGLVSPFGGQNSVDFSAISVVQQA
                     NIPARMLMSWTAPGSAAAMATSSGTSVVFDLADPKLGAYHDVYRDDADTDLTTAGTNP
                     TVSPASGNGFTGCALLNVSTGALSLYSTFAGFDSALNTAISGGAKLRVFAARGSFNDA
                     TATETATSCGAIVETPSAS"
BASE COUNT          360 a          721 c          761 g          358 t
ORIGIN      
        1 cgccgcgccc aagagcgtcg acccggtgaa ggcggagaag cgcctgcgcg aggcgctggc
       61 gcagatcgcg caggtgctgc agatcccggc cgcgcgcatc cactacaagc tgcgcaagtc
      121 gcagaagggc accgcgcaat atgccaagca ggacgaaagc ggcgtgttcc acatcgtcgc
      181 cgagcatggc tgcaagctgt gggtgaactt tgacgattac ctcgacaccg gcgtattcct
      241 cgaccaccgc ccgctgcgcc tgcgcatcca gcaggaagcg cgcggcagga aattcctcaa
      301 cctgttcgcc tataccggct cggccagcgt gcacgcggcg gtgggcggcg ccagctcaac
      361 cgtgtcggtc gacctgtcga acacctacct cgaatgggcc cagcgcaatc ttgaattgaa
      421 cggcttccat gccagggacg aggagcgccg cagctcgctg gaaaacgccc accgcctgct
      481 gcgcgccgac tgccgcgagt ggctggccga gcagtcggtg aaaagccgtc cgccgctgtt
      541 cgacctgatc ctgtgcgacc cgcccacctt ctccaactcc aagcgcatgg aaggcacgct
      601 cgacacgcag cgcgatcacg tggacctgat ccgccattgc gagaagctgc tggcgccggg
      661 tggcgtgctc tacttctcca ccaaccggcg cggcttcaag ctcgacagcg aggcgctggc
      721 cgggctggag atcaaggaca tcaccgcgca gacgctggac gaggacttca agcggccgcc
      781 gccggcgcac aagtgctggc gcatcgcgcg cggtgcgcaa taaaaaagcg ggggacggcg
      841 ccgccatccc ccgctcctct gcccctcact ccggttcagg gaaccggggt gttgcagggg
      901 caagagacga tgccggactc ggcgtgtgaa atgatcacgt tggcgctggc cacgcccggc
      961 gcgccgggca cgatcacgtg gatggcgttg accgagtagc tgttgacgtc cggctgcgat
     1021 tgttcgttga gaatgatggt ggcgactccg gggatggaaa tcttctggtt cggtttcccg
     1081 gaatacgcgt accccttgcc gttgatcttg agattgacga tggtcgagct gcccgacggc
     1141 gccaccgtct gcgagctctg gtcgcacacc gccgtggtgt cggcctcgat cacgtcggcg
     1201 gtgatcttca gcgcgctgat cgtcaccgcc agcttttcca cctcggcgtg cgacgacgcg
     1261 gtgccgttgg caccggaggt gatcgcgtcg atggtgctgg aatgcagccc cagcggcggc
     1321 gggttgtcga tctgcgccac ggtgtcttca cgcgtgccgc cggtcgggtc gaggtaaccg
     1381 gtgtcgccga gcaccacgtg ggcgttggcg gtcagcacct tgaccgtggc gtcgaccacg
     1441 gtggcgcggc ccgagtactg caccgggggc acggtgtcgt cggcacgtgc ggtgggcacg
     1501 gcagccgcgc cggcgaacag catgcccgcg acggccattc gcctgtagta gagagatgga
     1561 tgttgcatgg gtaaatcctc ctggtcatga cactgggtga atcaatggaa ccggcggcgc
     1621 gtcctcgcgg gcaggccgcg ccgccggcat cccgctgctt acgaagccga tggcgtctcc
     1681 acgatggcgc cgcagctggt ggcggtctcc gtggcggtgg catcgttgaa gctgccgcgg
     1741 gccgcgaaca cgcgcagctt ggcgccgccg ctgatggcgg tgttgagcgc gctgtcgaag
     1801 ccggcgaagg tggaatacag cgacagcgcg ccggtgctga cgttgagcaa cgcgcagccg
     1861 gtgaagccgt tgccgctggc cggcgacacc gtcgggttgg tgcccgccgt ggtcaggtcg
     1921 gtgtcggcat cgtcgcggta cacgtcgtgg tacgcgccga gcttgggatc agccaggtcg
     1981 aacaccacgc tggtgccgct gctggtggcc atggctgcgg ccgaacccgg cgccgtccag
     2041 ctcatcagca tgcgggccgg gatgttggcc tgctgcacga cgctgatggc ggaaaagtcc
     2101 accgagttct gcccgccgaa cggactcacc agtccattca cccgcagcag gtcacccgag
     2161 gcggcgccgg tcagcgccgg gatgccgacc acgtagctcg
//
LOCUS       DASZZC010000666         9034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_626008,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000666 DASZZC010000000
VERSION     DASZZC010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626008"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..815)
                     /locus_tag="VHE37_14135"
     CDS             complement(204..815)
                     /locus_tag="VHE37_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HVT36724.1"
                     /translation="MLGKLKIKMLERFYMRKAWREFRGVIEAALVPGSGGVAFIGDSL
                     THLGRWELMFPQAGARNFGIGGERAEHVLARLDPVIALRPRQAFLLIGTNDLAYGHGI
                     EDIARRVDEIIGRLAAALPDCRLHLQGLLPRQKKFSGRILQLNQLYEEIARRRGVAFI
                     DLFPRFADADGQLRAELTGDGLHLLGAGYQVWREELARWVRPA"
     gene            complement(826..3360)
                     /locus_tag="VHE37_14140"
     CDS             complement(826..3360)
                     /locus_tag="VHE37_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36725.1"
                     /translation="MPKSIVTPAVRLASAAAIAWLALGASATLAQEAPPSALDATSGD
                     GTPPAAADPASATPPGAAPAPAAEPASTGPAAATDPAPAAAPAQAAEVPIIPVREQAA
                     PAEASAAKATGQSVQLEDVVVTATKREKSLREIPASISEFKGDKLEKEGKLNLDDYLE
                     QSPGVTLSEFSPNFARVSMRGISTDTSGLAGLPSPTGFFIGDTAFSDPYVSNIQPDLS
                     AFDLAAVEVLKGPQGTLFGGAALAGAIRYLLNDPVMGEWQGRGFTQYIAPDGAPGGGK
                     PALTSGAVLNVPLYRDSLALRAALIRHNYPGITESTRYDEKGVDHGSGDQWRGILAWQ
                     PVDALKVKLTHIKQTFSAPNVNSNVDNPDTHSENYLVLSDPVSDQFTLDSGEISWDFS
                     GMKLISLTSHTTKDAHVFGDSTASLVGTNVPPNYPPAAGAFVTFNDDSHTLSEELRLQ
                     STGAGPFQWLAGAYLFNYRVHFDILLDTVAHQTALGNGSLLNNLAGGSGIVPVLFDKS
                     SLLYAVSEPWAKERALFFDASDVFWNHLELSTGARFYQTEVKGGFTGTGVLVDGVNNG
                     QDVDFESNDMVQKGINPKFAATWKFNRDYSVYLQAAKGFQFGGVNGVPSTPGNPVPPV
                     YKSSTLWNYEIGLRTAWLENTLHADLTIFDVEYKNPQLAQASSVTLGYGYTTNVGAAR
                     SKGIETSLRWLTPLPGLAFAFDGALIDSRTTVAFPNSSGAPVAPGMQMPGSEKSQYST
                     EFDYLVPAGPVNLGASVGYTYVGPGISDLVHPLRINDYGTLHAGISLNSDSWMLHPEL
                     SFTASNILNTTAPVTGASGTSIAQTPNITYVLNQPRTYTARLSLSF"
     gene            complement(3487..5004)
                     /locus_tag="VHE37_14145"
     CDS             complement(3487..5004)
                     /locus_tag="VHE37_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVT36726.1"
                     /translation="MAGASLPTTVERETRLGRVRGIERLGVETYRGLRYAVPVQRFRA
                     SAVHAQPWPGVYDATAYKAMAPQPPVLPEVSPPIEGGTFAEDCLFLNIHVPQAPAASP
                     RPVIFFIHGGSFTGGSGNFYDGTALAKGADAVVVCINYRLGVFAAFDLEWLGTERDGG
                     GQLWLGDQITALRWVRDNIADYGGNPGCVTVIGESAGAVSTAALCAAPEAEGLLHRAV
                     ACSTGYLLAEPSNDVVGVIAKTRRCTRAEAVAYLKSAPADELIRLQRRGKKLSPTPVA
                     NTPLLPGRMEDLIKARGSRAVPLIAGYATHEGQSLELMARLMIGLPWPLHELLLYIVA
                     RAIAMHGAKGREQVAGYLRRLKKATGSAGFGSNFHDVVWTDGFRRGAVEYCEMTSAAG
                     SRAWLYVMDVPMRFAGKTIRSSHGIDLALTFNVGDDPEHTVPAFADHPDATRLARRWV
                     QMLGHFARHGEPGTALGDWPAYTVPARPSMQIACDNCRVAHDVEALFRTSVWGQP"
     gene            complement(5080..5637)
                     /locus_tag="VHE37_14150"
     CDS             complement(5080..5637)
                     /locus_tag="VHE37_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36727.1"
                     /translation="MILAEALRLFAEHGYGGSSIRDIAKLVGIKGASLYSHYPSKAHL
                     LAELIRIGHDEHLQRIRAAMLGAGADPKLQLAALVRAHVLAHAEYPMLAVVANSEMHA
                     LPAEFAAPILEVRRQSESLLLDIINRGIALGKFAVAEPSLAVTAIGAMGLRVAHWYSP
                     EISKTPTQIADAYVEFACRVVGAKK"
     gene            complement(5849..6742)
                     /locus_tag="VHE37_14155"
     CDS             complement(5849..6742)
                     /locus_tag="VHE37_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36728.1"
                     /translation="MDDIPLIDPPVQAMTLADGRRLTWQEFGASAGNPVLYFHGGGSY
                     SLEAGIFHREAVRKNIRLIATNRPGTAGSSLRPGRLVAAYSDDLRELLDHLNIGKFAC
                     LGESNGGLVTMAVAATMPDRILGAMPINPTVPWFDPVARTVSSKAVAWGYRLMKWWPA
                     LLIRAAPLGVAHARRRRSEAGEGNGMFDPRDLAGPPPGTEPDIAELHWQVMEQSPKHA
                     SLMPELVWASSNWGFDYYSIPVPLDFFCGVHDAQAPFALVLADRNPDARFHYFSFGHH
                     GYSHPDARRRIFDQVASYFAA"
     gene            complement(6742..>9034)
                     /locus_tag="VHE37_14160"
     CDS             complement(6742..>9034)
                     /locus_tag="VHE37_14160"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT36729.1"
                     /translation="SGRFWYQRHTALGSEFVAVDAKTGKRRPAFDHAAVAAALNGALK
                     LDVPASAQNLGLQNARLSADGRKLTGVAGNKAVTADLEAHSVGMADITPAKPGLLLSP
                     TGTHGLKRAGDNLVLVDLASASERRLTEDGAPYYSYGKLPDSGLQTIPLKKSGAVLPP
                     YGAAFSPDGRFLICPRIDERRLGVDPFVEHVPTDGSLRPVVHEVRHSYTGDQAQMVTD
                     WFVFEVKTGSRHRIQLPLGSEPSAMLGNGVVGWSLQRQQVFILATTAASRSGALFRVD
                     LATGAAHALIDEAATTCRYEPNTLMYNMPNVYVTGDGAEVIWYSDRTGWGHLYRYDAQ
                     TGELLGQITGGNWMVFDIHHVDAAQRTLYFTAGGREAGVDPYYRHLYRVGFDGSGLML
                     LTGGELRADHVFAPNAAPLFKTLFGVPDAADKIRPAAGVFVDTYSTVDTPPVTVLRSL
                     HDGTLIAELERADVSALLDAGYRPPSRQLLKADDGKTDIPVVYYPPAKVMGKGAHPVI
                     DACYGGPQVYVAPRAYAEAAASSNPANRSAMSRLGFGVAVVDGRGTPGRGNAFRDAGF
                     PNFTEIGIRDHVAAIRQLAAQHPELDISRVGIHGWSWGGTFSAQAILAHGDFYSVCVT
                     GAGVYDYAALYPGFESSIGMPVYADGTNHPASVTEKPPSWDRLDITKMVDGLSGKMMI
                     VWGDADENVPPVQAYRLIEALIKANKPYDALILPNKTHPSASQSLNPYTAARTWDYFV
                     RHLLASTPPVAQPFQIQPRRVMQ"
BASE COUNT         1525 a         2916 c         2998 g         1595 t
ORIGIN      
        1 gcgggttcgc cgtcgacgat ggttacgagt taccgatgcc ctcggtgctg ctcagcgcgc
       61 gcaagcccga atgagtacga acgagcaaaa aaacgcctcc accgcaccgg catttcgtgt
      121 gttagttatt attttatata tttataatta gaatatattt tataacacgt tgaaatggca
      181 tgcggtggat gtcttttcat cgctcaggcc ggccgcaccc agcgcgccag ctcctcgcgc
      241 cacacctggt agcccgcgcc gagcaggtgc aggccgtcgc cggtgagctc cgcgcgcagc
      301 tggccgtcgg cgtcggcgaa ccgcgggaac aggtcgatga acgccacgcc gcgccggcgc
      361 gcaatttcct cgtacagctg gttcagctgc aggatgcgtc cgctgaattt tttctggcgc
      421 ggcaacagtc cctgcaggtg caaccggcaa tccggcaacg cggcagcgag acggccgatg
      481 atctcgtcga ctctccgcgc gatgtcttcg atgccatgtc cataggcaag gtcgttggtg
      541 ccgatcagga gaaatgcctg ccgcggccgc agggcgatga cggggtccag ccgcgcgagc
      601 acgtgctcgg cgcgttcgcc gccgatgccg aagttgcgtg cgcccgcctg cggaaacatc
      661 agctcccagc gcccgaggtg cgtgagactg tccccgatga aggccacgcc gccgctgcct
      721 ggcaccagcg ccgcctcgat cacgccgcgg aattcgcgcc acgccttgcg catgtagaag
      781 cgctccagca tcttgatctt gagtttgcct agcacggtga tttcctcaga aggacaggct
      841 caggcgcgcg gtgtaggtgc gcggctggtt gagcacgtag gtgatgttcg gcgtctgcgc
      901 gatggacgtg ccgctggcgc cggtcaccgg cgccgtggtg ttgaggatgt tgctggcggt
      961 gaaggacagc tccgggtgca gcatccagga atcgctgttc agcgagatgc cggcgtgcag
     1021 cgtgccgtag tcgttgatgc gcagcggatg caccaggtcg gagatgcccg ggcccacgta
     1081 ggtgtagccc acgctggcgc ccaggttcac cgggccggcc ggcaccaggt aatcgaactc
     1141 ggtcgaatac tgcgacttct ccgagcccgg catctgcatg cccggcgcca ccggcgcgcc
     1201 gctcgagttc ggaaacgcga cggtggtgcg cgagtcgatc agagcgccgt cgaaggcgaa
     1261 ggccaggccc ggcagcggcg tgagccagcg caggctggtc tcgatgccct tgctgcgcgc
     1321 cgcgccgaca ttggtggtat agccgtagcc gagcgtcacg ctcgacgcct gcgccagctg
     1381 cgggttcttg tactcgacgt cgaagatggt cagatcggca tgcagcgtgt tttccagcca
     1441 ggccgtgcgc agtccgatct cgtagttcca cagcgtggat gacttgtaga ccggcgggac
     1501 gggatttccc ggcgttgacg gcacgccatt cacgccgccg aactggaagc ccttggccgc
     1561 ctgcaggtag accgaataat cgcggttgaa tttccaggtg gcggcgaact tcggattgat
     1621 gcctttctgc accatgtcgt tgctttcgaa atccacgtcc tgaccgttgt tcacgccgtc
     1681 gaccagcacg ccggtaccgg tgaagccgcc cttgacctcg gtctggtaga agcgcgcgcc
     1741 ggtcgacagt tccaggtggt tccagaacac gtcgctggca tcgaagaaca gcgcgcgctc
     1801 cttcgcccag ggctcgctca ccgcatagag caggctgctc ttgtcgaaca gcaccgggac
     1861 gatgccgctg ccgccggcca ggttgttgag cagactgccg ttgcccagcg cggtctggtg
     1921 cgccacggtg tcgagcagga tgtcgaagtg cacgcggtag ttgaacaggt atgcgccggc
     1981 cagccactgg aacggcccgg cgccagtgga ttgcagacgc agttcctccg agagcgtgtg
     2041 cgagtcgtcg ttgaaggtga cgaacgcgcc ggcggccggc gggtagttgg gcggcacatt
     2101 ggtgccgacc agcgaggcgg tgctgtcacc gaacacgtgg gcgtccttgg tcgtgtgcga
     2161 ggtcagcgag atcagcttca tgccggagaa atcccagctg atctcgccgc tgtcgagcgt
     2221 gaactggtcg ctgaccggat cggacagcac caggtagttc tcgctgtggg tgtccgggtt
     2281 gtcgacgttg ctgttgacgt tgggcgcact gaaggtctgc ttgatgtgcg tgagcttcac
     2341 cttcagcgca tcgaccggct gccacgcaag gatgcctctc cactgatcgc cgctgccgtg
     2401 gtcgacgccc ttttcgtcgt agcgcgtgct ctcggtaatg cccggatagt tgtggcggat
     2461 caaggccgcg cgcagcgcca ggctgtcgcg gtacagcggc acgttgagca ccgcgccgct
     2521 ggtgagtgcg ggcttgccgc cgccgggcgc gccgtccggc gcgatgtact gggtgaaccc
     2581 gcgcccctgc cactcgccca tgaccggatc attgagtagg tagcggatgg caccggccag
     2641 cgcggcgccg ccgaacagcg tgccctgcgg gcccttgaga acttccacgg ccgccaagtc
     2701 gaaggcgctc aggtccggct ggatgttgga cacatacgga tcggagaacg cggtgtcgcc
     2761 gatgaagaaa ccagtcggcg acggcaggcc ggccaggccg ctggtgtcgg tactgatgcc
     2821 gcgcatcgac acgcgcgcga agttgggcga gaactccgac agcgtcacgc cagggctctg
     2881 ctccaggtag tcatcgagat tgagcttgcc ctccttctcc agcttgtcgc ccttgaactc
     2941 ggaaatggaa gcgggaatct cgcgcagcga tttctcgcgc ttggtggcgg tgaccaccac
     3001 gtcctccagc tgcaccgact gccccgttgc cttcgctgca gatgcttctg ccggggccgc
     3061 ctgctcccgc accgggatta tcggcacctc cgcggcctgt gctggcgcgg cggcgggcgc
     3121 gggatctgtg gccgcggcag gtcccgttga tgcgggttcg gctgcaggcg ctggagcggc
     3181 gccgggcgga gttgcggacg ctggatcggc ggcagccggt ggcgtgccgt cgccggaagt
     3241 tgcatccagc gcggacggcg gcgcttcctg cgccagcgtg gcgctggccc ccagcgccag
     3301 ccacgcgatc gcggctgcgg atgccagccg cacggcgggc gtgacgatgc tcttcggcat
     3361 gcagattctc ctcactcaac atcgatatgg cgcgccaccg acttcgcggc ccgccgtgtt
     3421 ttttccggcg gctcctgccg cgctcccatt acccggcgcg atcagccgcg gagaagactc
     3481 cgcgcatcac ggctggcccc agacagaggt gcgaaacagt gcctcgacgt cgtgcgcaac
     3541 ccggcagttg tcgcaggcga tctgcatcga cggccgcgcc ggaaccgtgt acgcgggcca
     3601 gtcgccgagc gcggttcccg gttctccgtg gcgcgcgaaa tgccccagca tctgcaccca
     3661 gcggcgcgcc agccgcgtcg catccgggtg atcggcgaag gccggcaccg tgtgctccgg
     3721 gtcgtcgccg acgttgaagg tgagcgcgag atcgatgccg tggctggagc gtatggtctt
     3781 gcccgcaaag cgcatcggca catccatgac gtacaaccag gcgcggctgc cggcggccga
     3841 cgtcatctcg caatattcga cagcgccgcg gcggaagcca tcggtccaga ccacatcgtg
     3901 aaagttggaa ccaaagcccg cgctgccggt ggccttcttc aggcgccgca ggtagcccgc
     3961 gacctgctcc ctgcccttgg cgccgtgcat ggcaatcgcg cgggccacga tgtacagcag
     4021 aagttcatgc agcggccagg gcaagccgat catgagccgg gccatgagtt ccagggattg
     4081 cccttcatgg gtggcgtaac ccgcgatgag cggcacggcc ctgctgccac gcgccttgat
     4141 caggtcttcc atgcgccccg gcagcagcgg cgtgttggcc acgggcgtcg gcgaaagttt
     4201 tttcccgcgc cgctgcaggc ggatcagttc atcggcggga gcggacttga gatacgcgac
     4261 agcttcagcg cgcgtgcagc ggcgtgtctt cgctatcaca ccaaccacgt cgttcgacgg
     4321 ctcggccaag aggtagccgg tgctgcaggc caccgcgcgg tggagcaggc cctctgcttc
     4381 gggcgcggcg caaagtgccg cagttgaaac cgcgcccgcc gattcgccga tgacggtgac
     4441 gcacccggga tttccaccgt agtcggcaat gttgtcgcgc acccagcgca acgcggtgat
     4501 ctggtcaccg agccacagct ggccgccgcc atcgcgctcc gttcccagcc attccaggtc
     4561 gaatgcagcg aaaacaccca gccggtaatt gatgcacacg accacggcat cggcgccctt
     4621 cgccagcgcg gtgccgtcat agaaattgcc gctgccgccg gtgaagctgc cgccgtggat
     4681 gaagaagatc accggccgcg gcgacgcggc aggagcctgc ggaacatgga tgttcaggaa
     4741 caggcagtct tccgcaaacg tgccgccctc gatgggagga ctgacctccg gaagtactgg
     4801 cggctgcggc gccatggcct tgtacgcggt ggcgtcgtat acgcccggcc acggctgcgc
     4861 atggaccgcg gatgcgcgaa agcgctgcac cggaaccgcg taacgcagcc cgcggtaagt
     4921 ttcgacgccc aggcgttcga tgccgcgcac gcgcccgaga cgggtctcgc gttcaaccgt
     4981 ggtcggaagt gaagcacctg ccatgctgtt tgctctgggc gatgggacga tgggcagcgg
     5041 gcagcgggca agcgccggcc atgctggcat tgggcggatt tatttttttg cgcccacgac
     5101 ccggcacgcg aattccacgt aggcgtcggc gatctgcgtg ggcgtcttgc tgatctcggg
     5161 ggaataccag tgcgcgacgc gcaggcccat ggcgccgatc gccgtgaccg ccagcgaggg
     5221 ctccgccacc gcaaacttcc ccagggcgat gccgcggttg atgatgtcca gcagcagcga
     5281 ttccgattgc cgccgcactt ccaggatcgg ggcggcaaac tccgccggca atgcatgcat
     5341 ttcggaattg gccaccaccg ccagcatggg atattcggca tgcgccagaa catgcgcgcg
     5401 caccagtgca gccagttgca gcttcggatc ggcgcccgcg ccaagcatcg cggcgcggat
     5461 acgttgcagg tgctcgtcgt ggccgatcct gattaattcc gccagcagat gcgccttcga
     5521 cggatagtgc gaataaagcg aggcgccctt gatgccgacg agcttggcga tgtcacggat
     5581 ggatgagccg ccgtaaccat gctcggcaaa caggcgcagg gcctcggcca ggatcacccc
     5641 gcgcgatccg ccaggcgcag cgcccggcgg caggacattt ccctcggccc gcaggcgtcg
     5701 atggatgctg agatgtgttt gcatactaac ggtcgttagc tgaactgggc ggatgctatc
     5761 acgctgcatc tgctcagact atcgaccgtt agcttgcttt cttatcggcg ctcgcgcgcc
     5821 acccactgcc ggctgttgga gaaaatgttc aggcagcgaa gtaactcgca acctggtcga
     5881 aaatgcggcg cctggcgtcc gggtgtgaat agccatgatg cccgaaggaa aaatagtgga
     5941 accgcgcatc cgggttacgg tctgcgagca ccaatgcaaa tggcgcttgg gcatcgtgca
     6001 cgccgcagaa gaaatccagt ggcacgggta tcgagtagta atcgaatccc cagttcgacg
     6061 aagcccacac cagttcaggc atcaacgatg cgtgcttcgg tgactgctcc atcacctgcc
     6121 agtggagttc agcgatgtcc ggctcggttc ccggaggcgg cccggccaga tcacgcggat
     6181 caaacatgcc gttgccctca ccggcttccg atctgcgccg gcgcgcgtgc gccactccca
     6241 ggggcgccgc cctgatgagc agcgctggcc accatttcat caggcgatag ccccatgcca
     6301 cggctttgga ggacacggtt ctggccaccg ggtcaaacca aggcacggtg ggattgatcg
     6361 gcatggcccc gagtatgcgg tccggcatgg tcgcggccac ggccatggtg accagtccgc
     6421 cgttggattc acccaggcag gcgaattttc cgatgttcag gtggtcgagc aattcgcgca
     6481 ggtcgtccga gtacgccgcg accagccgac ccgggcgaag gctggaacct gcggtgcctg
     6541 ggcggttggt ggcgatcagc cggatgtttt tccgcaccgc ttcgcggtga aaaatgccgg
     6601 cctccaggct gtagctgcca ccgccgtgaa aatacaaaac cggatttcct gcggacgcgc
     6661 cgaattcctg ccaggtcagc cggcgcccat ccgccagcgt catcgcctgg acgggaggat
     6721 cgatcagggg aatgtcgtcc attactgcat cacccggcgc ggctggatct ggaacggctg
     6781 cgcaaccggt ggagtgctcg ccagcagatg ccggacgaaa tagtcccagg tgcgcgccgc
     6841 ggtatacggg ttgagcgact ggctggcgct tggatgcgtc ttgttcggca ggatcagcgc
     6901 atcgtagggc ttgtttgcct tgatcaatgc ttcgatcagg cggtaggcct gtaccggcgg
     6961 cacgttctcg tcggcgtcgc cccagacgat catcattttt ccgctgaggc cgtccaccat
     7021 tttcgtgatg tcgagcctgt cccacgacgg cggcttttca gtcacgctcg ccgggtggtt
     7081 cgttccatcc gcgtaaaccg gcatgccgat cgaggactcg aagcctggat agagcgcggc
     7141 gtagtcatag acaccggccc cggtcacgca gacggaatag aaatcaccgt gcgccaggat
     7201 ggcctgcgcg ctgaaggtgc cgccccacga ccagccgtgg atgccgacgc gactgatgtc
     7261 gagttcgggg tgctgcgccg ccagctgccg gatggccgcc acgtgatcgc gtatgccgat
     7321 ctcggtgaaa ttgggaaagc cggcatcgcg gaaagcattg ccgcggccgg gcgtgccgcg
     7381 gccatccacc acggcgacgc caaatcccag acgcgacatc gccgagcggt tggccgggtt
     7441 ggatgaagcc gctgcctctg cataggcgcg cggcgccacg tagacctgcg gaccgccgta
     7501 gcaggcgtca atgaccggat gcgcgccctt ccccatgacc ttcgcgggcg ggtaatacac
     7561 caccgggatg tcggtcttgc catcatcggc cttgagcaac tgacgcgacg gtggtcggta
     7621 acctgcgtcg agcagggccg acacgtctgc ccgttccagc tccgcgatga gcgtgccgtc
     7681 atgcagcgag cgcagcacgg ttaccggcgg tgtatcgacc gtggaatacg tgtcgacgaa
     7741 cacgccggcg gccggcctga tcttgtccgc cgcatcgggc acaccgaaca gtgtcttgaa
     7801 cagcggcgca gcgttgggcg caaaaacatg atccgcccgc agttcgccgc cggtcagcag
     7861 catcaacccg gagccgtcga agccgacgcg gtacaggtgc cggtaatacg ggtccacacc
     7921 ggcctcgcgg ccacccgcgg tgaaatagag cgtgcgctgc gcggcgtcga cgtggtggat
     7981 atcgaacacc atccagttgc cgccggtgat ctgccccagc agttcgccgg tctgcgcgtc
     8041 gtagcggtac aggtggcccc agccggtccg gtcggagtac cagatgactt ccgcgccgtc
     8101 gccggtcacg taaacattcg gcatgttgta catcagcgtg ttcggctcgt agcggcaggt
     8161 cgtcgccgct tcgtcgatca gcgcgtgcgc tgcgccggtc gccaggtcga ctcggaagag
     8221 cgctccgctc ctggaggcgg cggtcgttgc caggatgaag acctgctgac gctgcagcga
     8281 ccagccgacc acaccgttgc cgagcatggc cgacggttcg ctgcccagcg gcagttgaat
     8341 gcggtgtctc gatcccgttt tgacttcgaa cacgaaccag tccgtcacca tctgcgcctg
     8401 gtcgccggtg taggaatgcc gcacctcatg cactaccggc cggagcgatc cgtcggtcgg
     8461 cacgtgctcg acaaatggat caacgccgag gcggcgctca tcgatccgcg ggcagatcag
     8521 gaaacgaccg tctggtgaaa atgcagcgcc gtaaggcggc agcaccgcgc cgctcttctt
     8581 cagcggtatg gtctgcaggc cgctgtcggg cagcttgccg tagctgtagt acggcgcgcc
     8641 gtcttcggtc agccggcgct cgctggcgct ggccaagtca accagcacca ggttgtcccc
     8701 tgcgcgcttc aagccatggg tgcccgtcgg cgagagcaac agtcccggct ttgccggcgt
     8761 gatgtcggcc atgccgaccg agtgagcttc caggtcggcg gtgacggcct tgttacctgc
     8821 caccccggtg agtttgcggc cgtccgcact caggcgagca ttctgcaggc caagattctg
     8881 cgcgcttgcg ggaacgtcga gcttcagtgc gccgttcaac gctgcggcga cggccgcgtg
     8941 gtcgaacgcc ggtcggcgct tgccggtctt ggcgtccacc gcgacaaatt ccgaacccag
     9001 cgcggtgtgc cgctggtacc agaagcgacc gctg
//
LOCUS       DASZZC010000667         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_428012,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000667 DASZZC010000000
VERSION     DASZZC010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428012"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VHE37_14165"
     CDS             <1..662
                     /locus_tag="VHE37_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVT36730.1"
                     /translation="SIRAQGVVQPIVVRPSGTQFEIVAGERRWRAAKLAGLAAVPVVV
                     RTLDEKGAMAVALVENIQRADLNPLEESLALYKLIKECGLTHEKCAEAVGKSRAAVSN
                     LLRLMELNDDVQALVRAHRLSFGHAKVLLGTHGPRQSSLAKMAAEKEWSVRQLEALVQ
                     AEPAHKGKPKPHKGHKLEAELAQRIGLPVKLLQNPKGAGKLTVSFKSQDELDKLLKAL
                     H"
     gene            693..1481
                     /locus_tag="VHE37_14170"
     CDS             693..1481
                     /locus_tag="VHE37_14170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36731.1"
                     /translation="MDQYRTALITGASSGIGECFASALAAQGLDLILVARSRDKLHAT
                     AQALTKDHGVKVAVIAADLAQPGSGAALAAEVAKKHLTVDLLINNAGFGGAGIFHKNE
                     AARDQQMIALNIAAVVDLAHAFLPAMVERGRGGILNVASLAGFQPTPYMTVYGATKAF
                     VLSFSEGLWAEYRDKGIQVMALCPGPVDTPFFEATGSGAKLRSTVPRGTMLTAEQVVK
                     EALAGFRRGKSFVVPGMAMKLVSWLPRLVPRSVVAAGAARTMKR"
     gene            1717..2097
                     /locus_tag="VHE37_14175"
     CDS             1717..2097
                     /locus_tag="VHE37_14175"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HVT36732.1"
                     /translation="MTPLQRWGSTVGTVIILQWGLVLALAAAAWLVGGFSAARSLFFG
                     GAAVALPNMVLALWLTARVHRTGIAGRAALLMGEVLKLGLIVACLVTIVARYRPELVW
                     LALIVGVVVALKAQWLALWMTRRY"
     gene            2120..2998
                     /gene="atpB"
                     /locus_tag="VHE37_14180"
     CDS             2120..2998
                     /gene="atpB"
                     /locus_tag="VHE37_14180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004477.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVT36733.1"
                     /translation="MAVENAEQVAEAAGNAEHALTPASYIGHHLSFLDKSVGSGGFWT
                     LHLDTLAVSVLLGIVTLGFLRWVVSGATAGVPGKRQAFVEICVDFVNDQVKSLFHHDI
                     SFVAPLALTTVLWVVMMNAMDFLPVDIIGHGLQAVGLHEFRIVPTADVNMTFALALSV
                     WLLMIGTAISVKGAGGFVHELFCTPFGVPKLSLNPFTWIGAVLMLAVNFLFQMIEYLS
                     KPLSHSLRLFGNMYAGEIIFLLLWMWASTGLVGTVFGSLLGVGWIIFHILIVALQAYI
                     FMMLTVVYISMAHESH"
     gene            3076..>3222
                     /gene="atpE"
                     /locus_tag="VHE37_14185"
     CDS             3076..>3222
                     /gene="atpE"
                     /locus_tag="VHE37_14185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HVT36734.1"
                     /translation="MIQAYTSVGIGLIIGLGALGACVGVGIMCSRFLEGAARQPELAP
                     MLQAK"
BASE COUNT          541 a         1004 c         1082 g          595 t
ORIGIN      
        1 gttcgatccg cgcgcagggc gtggtgcagc cgatcgtggt gcggccgtcc ggcacgcagt
       61 tcgagatcgt cgccggcgag cgccgctggc gcgcggcgaa actcgccggc cttgccgccg
      121 tgccggtggt ggtgcgcacg ctcgacgaga agggcgcaat ggcggtggcg ctggtggaga
      181 acatccagcg cgccgatctc aacccgctgg aagaatcgct ggcgctgtac aagctgatca
      241 aggaatgcgg actgacgcac gagaaatgcg cggaggcggt gggcaagtcg cgcgccgcgg
      301 tgtcgaacct gctgcgcctg atggaactca acgacgacgt gcaggcgctg gtgcgtgcgc
      361 acaggctgtc gttcggccac gccaaggtgc tgctgggcac acacggtccg cggcagtcat
      421 cgctggcgaa gatggcggcg gagaaggaat ggtcggtgcg ccagctggag gcgctggtgc
      481 aggccgagcc cgcgcacaag ggcaagccta aaccgcacaa gggacacaag ctggaagccg
      541 agctcgcgca gcgcatcggc ctgccggtga agctgctgca gaatcccaag ggcgccggca
      601 agctgacggt gtcgttcaag tcgcaggatg agctggacaa gttgctcaag gcgctgcact
      661 aacgcagccc gttgcaggca cggaggcgaa tcatggacca gtaccgcacc gcgttgatca
      721 ccggcgcttc gtcggggatc ggcgaatgtt tcgccagcgc gctggccgcg caggggctcg
      781 acctgatcct ggtggcgcgc tccagggaca agctgcacgc cactgcgcag gcgctgacca
      841 aggatcacgg cgtgaaagtc gcggtgatcg cggccgacct ggcgcagccc ggcagcggcg
      901 ccgcgctggc cgccgaggtg gcgaagaagc atctcaccgt cgacctgctg atcaacaatg
      961 ccggcttcgg cggcgccggc atcttccaca agaatgaagc ggcgcgcgac cagcagatga
     1021 tcgcgctcaa catcgcggcg gtggtcgatc tggcgcatgc gttcctgccg gcgatggtcg
     1081 agcgcgggcg cggcggcatc ctcaacgtgg cttcgctcgc cggcttccag cccacgccat
     1141 acatgacggt gtacggcgcg accaaggctt tcgtgctgtc gttctccgag gggctgtggg
     1201 cggagtaccg cgacaagggc atccaggtga tggcgttgtg cccggggccg gtcgacacgc
     1261 cattttttga ggcgaccggc agcggcgcca agctgcgcag cacggtgccg cgcggaacca
     1321 tgctgacggc ggagcaggtg gtgaaggaag cgctggccgg gttcaggcgt ggcaagagtt
     1381 tcgtggtgcc aggtatggca atgaagctgg tgagctggct gccgcggctg gtgccgcgtt
     1441 ccgttgtcgc cgccggcgca gcgcgcacca tgaagcgctg accgggcaat tttcgaggca
     1501 accgcaccac cggtcggctt gttattgttt attaaatata tttaaattaa atcaataatt
     1561 aataacatca taatggcctt gcggtgaagc attcctgcgg ccagaaaacg cgcaacaacc
     1621 gctgccggca ttttcattga ccccgcttgg tgcaatcagt aaactgcgcg ccccgccgcg
     1681 gtacccgccg gaatttcacg tggatcgtcc tgcaacatga ccccgctgca aagatggggt
     1741 tcgacggtcg gcacggtgat catcctgcaa tgggggctgg tgctcgcact ggctgccgcg
     1801 gcgtggttgg tgggtggttt ttccgcggcg cgttcgttgt ttttcggcgg cgccgcggtg
     1861 gcgctgccca acatggtgct cgcgttgtgg ctcaccgcgc gcgtgcaccg cacggggatt
     1921 gcggggcgcg ccgcgttgtt gatgggcgag gttctcaagc tgggcctgat cgtcgcctgc
     1981 ctggtgacca tcgtcgcccg ctaccggccg gagctggtgt ggctggcctt gattgtcggg
     2041 gtggtcgttg cgctcaaggc gcagtggctc gcgctgtgga tgacacggcg ctactgaaaa
     2101 cagattgcat agactcgtta tggccgtcga aaacgcagag caggtcgctg aagcagcggg
     2161 caacgccgag cacgcgctca ctcccgccag ttacatcggc catcacctct cgttcctcga
     2221 caagtccgtc ggcagcggcg gtttctggac gctgcacctg gacacgctgg ccgtctccgt
     2281 cctgctcggc atcgtcacgc tgggcttcct gcgctgggtg gtgagcggcg ccaccgccgg
     2341 cgtgccgggc aagcgccagg ccttcgtcga gatctgcgtc gacttcgtca acgaccaggt
     2401 caaaagcctg ttccaccacg acatcagctt cgtcgccccg ctggcgctga ccaccgtgct
     2461 gtgggtggtg atgatgaacg ccatggactt cctgccggtg gacatcatcg gccacggcct
     2521 gcaggcggtc ggcctgcacg agttccgcat cgtgcccacc gccgacgtca acatgacctt
     2581 cgcgctggcg ctgtcggtgt ggctgctgat gatcggcacc gccatcagcg tcaagggcgc
     2641 cggcggtttc gtccacgaac tgttctgcac gccgttcggt gtgcccaagc tgagcctcaa
     2701 cccgttcacc tggatcggcg ccgtgctgat gctggcggtg aacttcctgt tccagatgat
     2761 cgagtacctg tccaagccgc tgtcgcacag cctgcgactg ttcggcaaca tgtacgccgg
     2821 cgaaatcatc ttcctgctgc tgtggatgtg ggcctcgacc ggcctggtcg gcaccgtgtt
     2881 cggctcgctg ctgggcgtgg gctggatcat tttccacatc ctgatcgtcg cgctgcaggc
     2941 ctacatcttc atgatgctga ccgtcgtcta catctcgatg gcgcacgaga gtcactgaaa
     3001 aagatttttc cacccaccac acctgctatt taagtcacag aggaaactgc aatggatcac
     3061 gttcaattcc tggccatgat tcaggcctac accagcgtcg gcatcggcct gatcatcggc
     3121 ctcggcgcac tgggcgcctg cgtcggcgtc ggcatcatgt gctcgcgctt cctggaaggc
     3181 gccgcgcgcc agccggaact ggccccgatg ctgcaggcca ag
//
LOCUS       DASZZC010000668         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_428048,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000668 DASZZC010000000
VERSION     DASZZC010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428048"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..963
                     /locus_tag="VHE37_14190"
     CDS             97..963
                     /locus_tag="VHE37_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVT36735.1"
                     /translation="MGVIWLVWGSTYLAIRIAVLQLPPVLFAGARFALAGVLMFVYAW
                     LRGARLPRSRVDWQRIAVSGFALLVAGNGLVTWSEQTVESNQAALIVATSALWIAGFG
                     VLGASGERVTPLTLAGLLLGFAGVAVLVGSGITARLAPVSAYCALLLAPLMWSAGSIY
                     SRRHPVSVAPAMTAALQMCISGVVLGALGFSLGEAPRWHAQPQAYAALAYLVVFGSCV
                     AYGAYYWLVHEVTPATLGTYAYVNPAVAVLLGGWLLDERLTTAQVWGTVIILSGVVLV
                     TLSARRRSGAAK"
     gene            1106..>1852
                     /locus_tag="VHE37_14195"
     CDS             1106..>1852
                     /locus_tag="VHE37_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HVT36736.1"
                     /translation="MSAPAHAQERVLRAGNGTEPATLDPHKAEGVSDANILRDLYEGL
                     TSISPRGEVIPGAAQGWEVSADGRDYVFHLRPDARWSNGDELTAEDFVAGLRRSADPQ
                     TGSSYSEILAPIEHATEVTRGQLPPAQLAVDAVDEHTLHIRLKAPTPYFLGLLSHSTT
                     YPVHRPTLAKYGAAFARPGRLVSNGAYQLSDWVVQSQVTLTRNPHYWDNAHTQIDRVI
                     YYPTEDLNAEIKRYRAGELDYTYEIPLVQAN"
BASE COUNT          271 a          625 c          614 g          342 t
ORIGIN      
        1 gctccatgcc ggcgttttgt tttgccgacc caccaccgcg cctgcacgcg cacgtccgca
       61 gccatggcca gccgttccgc acaagtcgtc gccgcaatgg gagtcatctg gctggtctgg
      121 ggctcgacct acctcgccat ccgcatcgcc gtgctgcaat tgccgccggt gctgttcgcc
      181 ggcgcgcgct tcgccctggc cggcgtgctg atgttcgtct acgcctggct gcgcggcgcg
      241 cgcctgccgc gcagccgggt ggactggcag cgaatcgcgg tgagcggctt cgcgctgctg
      301 gtcgccggca acgggctggt gacgtggagc gaacagacgg tggaatccaa ccaggcggcg
      361 ctgatcgtcg ccacctccgc gctgtggatc gccggcttcg gcgtgctggg cgcaagcggc
      421 gagcgcgtca cgccgctcac gctggccggc ctgctgctgg gattcgccgg cgtggcggtg
      481 ctggtgggca gcggcatcac cgcgcgcctc gctccggtgt ccgcgtactg cgcgctgctg
      541 ctggcgccgc tgatgtggtc ggcgggttcg atctattcgc ggcgccatcc ggtcagcgtt
      601 gcgccggcga tgaccgcggc gctgcagatg tgcatcagcg gcgtcgtgct gggcgcgctg
      661 ggattctcgc tgggcgaagc gccgcgctgg cacgcgcagc cgcaggcata cgcggcgctt
      721 gcctacctgg tcgtgtttgg ctcgtgcgtg gcttacggtg cttactactg gctggtgcac
      781 gaagtcacgc ctgccacgct cggcacctac gcctacgtga atcccgccgt cgccgtgctg
      841 ctcggcgggt ggctgcttga tgagcgcctg accacggccc aggtgtgggg caccgtcatc
      901 atcctctccg gcgtggtgct ggtgacgcta tcggcacggc gcaggtccgg cgcggcaaaa
      961 taacccggct tttcagactg accgcactgc cattttgata gttattaatt attaattgtt
     1021 ttcaaaatta tacaataatt taaaacatcg ccggacaggt tgcggtagga ggttttttgc
     1081 gcctgcgctg ctggtgatgt cgttgatgtc cgctcctgcc cacgcgcagg agcgcgtgct
     1141 gcgcgccggc aacggcaccg agccggccac gctcgacccg cacaaggccg agggcgtgtc
     1201 cgacgccaac atcctgcgcg acctgtacga aggcctcacg tccatctcgc cgcgcggcga
     1261 ggtgatcccc ggcgcggcgc agggctggga ggtgagcgcc gatggccgcg actacgtttt
     1321 tcatctccgg ccggacgcgc gctggtccaa cggcgatgag ctgacggcag aagatttcgt
     1381 cgccggcctg cgccggtccg ccgacccgca gaccggatcg tcgtattcgg aaatccttgc
     1441 gccgatcgaa catgctaccg aggtgacgcg cgggcagttg ccgccggcgc agctggcggt
     1501 cgatgcagtg gatgaacaca cgctgcacat ccgcctgaag gcgcccacgc cgtattttct
     1561 cgggctgctc agccactcga ccacttaccc ggtccatcgc ccgacgctcg ccaagtacgg
     1621 tgctgccttc gcgcggccgg gccgactggt gagcaacggc gcttaccagc tgagcgactg
     1681 ggtggtgcag tcgcaggtca cgctcacgcg caatccgcac tactgggaca acgcgcatac
     1741 gcagatcgat cgcgtcatct actacccgac cgaggatctc aacgccgaga tcaagcgcta
     1801 tcgcgcgggc gaactcgact acacctacga gatcccgctg gtgcaggcca ac
//
LOCUS       DASZZC010000669         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_112778,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000669 DASZZC010000000
VERSION     DASZZC010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112778"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VHE37_14200"
     CDS             <1..461
                     /locus_tag="VHE37_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVT36737.1"
                     /translation="TFPLPESQLDRFLMRISLGYPDAAAERALLSERERRDLLNELPP
                     ALTPESLLGLQTQVNSIKTQPALLDYVLALVQHTRQHAGLRQGLSPRAGLSLRRAAQA
                     WALIDGRPGVIPEDVQAVFPAVVNHRLIAVSERDAGAPTAEEILAKVAIP"
     gene            472..1539
                     /locus_tag="VHE37_14205"
     CDS             472..1539
                     /locus_tag="VHE37_14205"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HVT36738.1"
                     /translation="MRFWLILKILLHPIRYAQERIDRWVMARVKRQAGPITISRRRVY
                     ILPTRFGYGFGIMTVAMLLGAMNYSNSMAFMLTFLLIALGLVCMHHTHANLVNVELRS
                     GHAPPVFAGEVAHFEVLVDNSAGKPRYSMELSWPHYTVPLAPLDGDGRPIAGRRRRRG
                     DEDPPPITADVAAQSATSMKIGLPAAQRGWLQARVFALGTEFPLGLFHAWTWAELDMN
                     CLVYPRPASAGIPPPPASGIGGLLSQNRLGQDEFAGLRNYQRGDTFKAIHWKSLPKLR
                     SPMVKLFAESMEQELWLDWDSLHGLDAEERLSQLTRWVLDAESENRAYGLRVPGTTLN
                     PARGLQHQHLCLRTLALFEAP"
     gene            1536..>1749
                     /locus_tag="VHE37_14210"
     CDS             1536..>1749
                     /locus_tag="VHE37_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HVT36739.1"
                     /translation="MTGDHLKLASLLRLLSVMALVLAPHLVRLPLWESAAVAALLGWR
                     ALAAMRQWPLPSPAVRLALTLLPTAGL"
BASE COUNT          254 a          622 c          593 g          280 t
ORIGIN      
        1 gcacctttcc actgccggag tcgcagctgg accgcttcct catgcgcatc tcgctcggtt
       61 accccgacgc ggcagcggaa cgcgcgctgc tgtcggagcg cgagcggcgt gacctgctca
      121 acgaactgcc gcccgcgctg acacccgaat cgctcctcgg tctgcagacg caggtgaact
      181 cgatcaagac gcagccggcc ctgctcgatt acgtgctggc gctggtccag cacacgcgcc
      241 agcacgcggg gctgcggcag ggcctgtcgc cgcgcgctgg gttgtcgctg cgccgcgcgg
      301 cgcaggcctg ggccctgatc gatgggcgcc cgggcgtcat tcccgaggat gtgcaggcgg
      361 tgttcccggc cgtggtcaac caccgactca tcgccgtgtc cgagcgcgat gccggcgcgc
      421 ccacggccga ggaaatcctg gccaaggtgg cgatcccctg atcgtcaacg catgcgtttc
      481 tggctgatcc tcaagatcct gctgcacccg atccgctacg cgcaggagcg catcgaccgc
      541 tgggtgatgg cgcgggtcaa gcgccaagcc gggccgatca ccatctcgcg acggcgcgtg
      601 tacatcctgc ccacgcgctt cggctatggc ttcggcatca tgaccgtggc catgctgctg
      661 ggtgcgatga actacagcaa cagcatggct ttcatgctca ccttcctgct catcgcgctg
      721 ggcctggtgt gcatgcatca cacgcacgcc aacctggtga acgtggaatt gcgcagcggg
      781 cacgcgccgc ccgtgtttgc cggcgaggtg gcgcacttcg aggtgctggt cgacaacagt
      841 gcaggcaagc cgcgctacag catggagctg tcgtggccgc actacaccgt gccactggcg
      901 ccgctggatg gcgacggccg gccgattgca gggcgccggc gccgccgcgg cgatgaagat
      961 ccgccgccga tcaccgccga cgtggcggcg cagtcggcca ccagcatgaa gatcggtttg
     1021 cccgcagcac agcgcggctg gctgcaggcg cgcgtgttcg cgctgggcac ggaatttccg
     1081 ctcggactgt tccatgcctg gacctgggcc gagctcgaca tgaactgcct ggtgtatcca
     1141 cgcccggcca gcgctggcat tccgccgccg cccgccagcg gcatcggcgg cctgctgtcg
     1201 cagaatcgtc tcggccagga cgagttcgcc ggcctgcgca attaccagcg cggcgacacc
     1261 ttcaaggcga tccactggaa gagcctcccc aagctgcgct cgccgatggt caagctgttc
     1321 gccgagagca tggagcagga actgtggctc gactgggaca gcctgcacgg cctcgatgcc
     1381 gaggagcgcc tgtcgcagct cacgcgctgg gtgctcgacg ccgagagcga aaaccgcgcc
     1441 tacggcctgc gcgtgcccgg caccacgctc aaccccgcgc gcgggctgca gcaccagcac
     1501 ctgtgcctgc gcacgctggc gctgttcgag gcgccatgac gggcgaccat ctcaagctgg
     1561 cctcgctgct gcgcctgctg tcggtgatgg cgctggtgct ggcgccgcac ctggtgcggc
     1621 tgccgctgtg ggagagcgct gcggtggcag cactgctcgg ctggcgcgca ctggcggcga
     1681 tgcgccagtg gccgctgccc tcgccggccg tgcgcctggc actgacgctg ctaccgaccg
     1741 ccggcctgg
//
LOCUS       DASZZC010000670         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_960682,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000670 DASZZC010000000
VERSION     DASZZC010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960682"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VHE37_14215"
     CDS             <1..390
                     /locus_tag="VHE37_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVT36740.1"
                     /translation="GKGRKERTVHVGAQARAALGEWLRVRGSYALQDEPALFVSTRGT
                     RLSRNMIGVRLKVWARAAGLEAQLHPHRLRHSFATHLLEGSGDLRAVQELLGHANLAT
                     TQVYTHLNWKHLAQVYDGAHPRARRKP"
     gene            541..783
                     /locus_tag="VHE37_14220"
     CDS             541..783
                     /locus_tag="VHE37_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36741.1"
                     /translation="MRNVLIALSLVSAVALSACGKKEEAAPAPVAAPAPAPAPAAQQA
                     ADAAAAPAATTTDTTSAAPSAPTTTTTTSTDAAKPQ"
     gene            906..1445
                     /gene="hslV"
                     /locus_tag="VHE37_14225"
     CDS             906..1445
                     /gene="hslV"
                     /locus_tag="VHE37_14225"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HVT36742.1"
                     /translation="MRQFDGTTILCVRKGGQVALAGDGQVSMQNTVVKGNARKVRRLY
                     NDKVIAGFAGGTADAFTLFERFEGKLEKHSGNLTRAAVEMAKDWRTDRYLRRLEALLL
                     VADKDTSLMISGNGDVMEPEAHGVLAIGSGGSYAAAAARALVDNTQLSAREIAEKALH
                     IAADICIYTNHSLTIETIG"
     gene            1442..2806
                     /gene="hslU"
                     /locus_tag="VHE37_14230"
     CDS             1442..2806
                     /gene="hslU"
                     /locus_tag="VHE37_14230"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HVT36743.1"
                     /translation="MNDISASVTPATMTPREIVQELDRHIVGQAAAKRAVAIALRNRW
                     RRQQVDGPLRAEIHPKNILMIGPTGVGKTEIARRLAKLVNAPFVKVEATKFTEVGYVG
                     KDVDSIIRDLMDAAIKQTREQATRRVRDKAEVAAEERILDVLLPPPKDFGEASPQRQS
                     ENGAARQVFRKQLREGKLDDTEIEIKLAAPNARVELMGPPGMEEMTSQLQSLFANMNN
                     GGKQKTKKMKIKEALAQAVEEEAAKLVDEEAIRVEALRNVEENGIVFIDEIDKVCKRS
                     EFVGADVSREGVQRDLLPLVEGSTITTKYGAVKTDHILFIASGAFHMAKPSDLIPELQ
                     GRLPIRVELDALKSEDFVRILTEPNYSLTEQYRALMKTEGVEIDFTREGLARIAEVAW
                     QVNEGTENIGARRLHTVMERLMEEIAYEAPDKAGTTLTIDAAYVDAKLARLAGNQDLS
                     RYIL"
     gene            2932..3345
                     /locus_tag="VHE37_14235"
     CDS             2932..3345
                     /locus_tag="VHE37_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HVT36744.1"
                     /translation="MTTARVTTLHHRRQSRVLEVTFDDGVRHALPCEYLRVFSPSAEV
                     RGHGVGEPMLVGGKREVNIAAIEPVGNYAVRLRFSDGHDTGLYSWDVLRALGDGQEKN
                     WARYLERLEQAKMSRDRDVATLAALPKKYLPPAQR"
BASE COUNT          647 a         1134 c         1102 g          505 t
ORIGIN      
        1 ggcaaggggc gcaaggagcg cacggtgcat gtcggcgccc aggcgcgcgc cgcgctgggc
       61 gaatggctgc gcgtgcgcgg cagctacgcg ctgcaggacg agccggcgct tttcgtgagc
      121 acgcgcggca ctcgcctgtc gcgcaacatg attggcgtgc gcctgaaggt gtgggcgcgc
      181 gcggccgggc tcgaggcgca gctgcatccg caccgcctgc gccattcctt cgccacgcac
      241 ctgctcgagg gcagcggcga tttgcgtgcc gtgcaggagc tgctcggcca cgccaatctc
      301 gccacgacgc aggtgtacac ccacctgaac tggaagcatc tggcgcaggt ctatgacggt
      361 gcgcatccgc gggcgcggcg caagccgtag caccgccatg cgcaccggtc ttcggtggca
      421 gcgcgacccc tgcttgcgct cttgtgcagc gcaaaaaaat gggcataatg cgcggcccgc
      481 cggcggaacg actgatcatc gtccgctggt tttttaataa cactccatgg agctacaaca
      541 atgcgtaatg ttctgattgc cctgagcctg gtttccgccg tcgcgctgtc cgcctgcggc
      601 aagaaggaag aagccgcccc ggcccctgtc gccgccccgg ctcccgctcc ggctccggcc
      661 gcccagcagg ccgccgatgc cgccgctgct ccggctgcca ccaccaccga caccacctcg
      721 gctgctccga gcgcgccgac caccacgacc accacgtcga ccgacgccgc caagccgcag
      781 taaccctgca gctggcgtca caggaaaccc ggccttgcgc cgggtttttt gttgcccggc
      841 gcgcggcttg aaacacccgt cccgcgcccc caccatcggt tgcattcccc cacaccggat
      901 tcccgatgag acagttcgac ggcaccacca tcctgtgcgt gcgcaagggc ggccaggttg
      961 cactggccgg tgacggccag gtgtcgatgc agaacaccgt ggtcaagggc aatgcgcgca
     1021 aggtgcgccg gctctacaac gacaaggtga tcgccggatt tgccggcggc accgccgacg
     1081 ccttcaccct gttcgagcgc ttcgaaggca agctggaaaa acacagcggc aatctcaccc
     1141 gagcggcggt ggagatggcc aaggactggc gcaccgaccg ctacctgcgc cggcttgagg
     1201 cgctgctgct ggtcgccgat aaagacacct cgctgatgat ctccggcaac ggcgacgtga
     1261 tggagccgga agcgcacggc gtgctggcca tcggctccgg cggttcctac gccgccgccg
     1321 cggcgcgcgc tctggtggac aacacgcagc tgtccgcgcg cgagatcgcc gagaaggcgc
     1381 tgcacatcgc cgccgacatc tgcatctaca ccaaccacag tctcaccatc gagacgatcg
     1441 gatgaacgac atttctgcca gcgtcacccc ggccacgatg accccgaggg aaatcgtcca
     1501 ggaactagac cgccacatcg tcggccaggc cgcggccaag cgcgcggtcg ccatcgcgct
     1561 gcgcaaccgc tggcgccggc agcaggtcga cggcccgctg cgcgcggaaa tccatccgaa
     1621 gaacatcctg atgatcgggc ccaccggcgt cggcaagacc gagatcgcgc ggcgcctggc
     1681 caagctggtc aacgcgccgt tcgtgaaagt cgaggcgacc aagttcaccg aggtcggcta
     1741 cgtgggcaag gatgtcgaca gcatcatccg cgacctgatg gatgccgcga tcaagcagac
     1801 gcgcgagcag gccacgcgcc gtgtgcgcga caaggccgag gttgccgccg aggagcgcat
     1861 cctcgacgtg ctgctgccgc cgccgaagga tttcggcgag gccagcccgc agcgccagag
     1921 cgaaaacggt gccgcgcgcc aggtgttccg caagcagctg cgcgaaggca agctcgacga
     1981 caccgagatc gagatcaagc tggccgcgcc caacgcgcgc gtcgagctga tgggcccgcc
     2041 gggcatggaa gagatgacca gccagttgca gtcgctgttc gccaacatga acaacggcgg
     2101 caagcagaag accaagaaga tgaagatcaa ggaagcgctg gcgcaggccg tcgaggaaga
     2161 ggcggccaag ctggtggacg aggaagccat ccgcgtcgag gcgctgcgca acgtcgagga
     2221 gaacggcatc gtcttcatcg atgaaatcga caaggtgtgc aagcgcagcg agttcgtcgg
     2281 cgccgacgta tcgcgcgaag gcgtgcagcg cgacctgctg ccgctggtgg aaggctcgac
     2341 catcaccacc aagtacggcg cggtgaagac cgatcacatc ctgttcatcg ccagcggcgc
     2401 gttccacatg gccaagccca gcgacctcat cccggaactg cagggccgcc tgccgatccg
     2461 cgttgaactc gacgcgctca agagcgagga cttcgtgcgc atcctcaccg agccgaacta
     2521 ctcgttgacg gaacagtacc gcgcgctgat gaagaccgag ggcgtggaga tcgacttcac
     2581 cagggaaggt ctggcgcgca tcgccgaggt ggcctggcag gtgaacgagg gcaccgagaa
     2641 catcggtgcg cgccgcctgc acacggtgat ggaacggctg atggaagaga tcgcgtacga
     2701 ggcgccggac aaggcgggca ccacgctcac catcgatgcc gcgtatgtcg atgccaagct
     2761 ggccaggctc gcgggcaacc aggatttgag ccgctatatc ctctgaacag cctccaccgc
     2821 agcgctgctt tgcttgttat aaaatattgt tcaaatgtca aaatatataa ttgtttataa
     2881 catgctgtat gcgggtgcgg tggaactgtt ttcgtgcgct tccaccgcgc catgaccacc
     2941 gccagagtca ccacgctgca tcaccgccgc cagtctcgcg tactggaagt cactttcgat
     3001 gacggcgtgc gccatgcgct tccgtgcgaa tacctgcgcg tgttttcgcc gagcgcggaa
     3061 gtgcgcggcc atggcgtcgg cgagccgatg ctggtgggcg gcaagcgcga ggtgaacatc
     3121 gccgcgattg agccggtcgg caattacgcc gtgcgcctgc gcttcagcga cggccacgac
     3181 accggcctgt attcgtggga cgtgctgcgc gcgctcggcg acgggcagga aaaaaactgg
     3241 gcgcgctatc tcgagcggct ggagcaggcg aagatgtcgc gcgaccgcga cgtcgccacg
     3301 ctggcggcgc ttccgaagaa gtacctgccg ccagcgcagc gctgactcgc cgtcaggagc
     3361 aggccttgat ggccaccttg ggcaggcc
//
LOCUS       DASZZC010000671         4717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_877358,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000671 DASZZC010000000
VERSION     DASZZC010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877358"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..452
                     /locus_tag="VHE37_14240"
     CDS             153..452
                     /locus_tag="VHE37_14240"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HVT36745.1"
                     /translation="MDGEIRHEYDGTTFVWRAAKAAANLAKHGVSFDEATTVFSDPLL
                     RLVDASRNDEARDAVIGFDLQARLLFVVHVEIDGEAIRLISARRASRDEEDLYAQ"
     gene            442..729
                     /locus_tag="VHE37_14245"
     CDS             442..729
                     /locus_tag="VHE37_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36746.1"
                     /translation="MLNDKLKQRLRRDRPSVSITLRIPQDVVDTLKELAPRLGMSGYQ
                     TLLKSYISEGLRRDEARMVGVSGQKLIAALRRHGVSAKVLDAALHDLEKAA"
     gene            complement(732..1805)
                     /locus_tag="VHE37_14250"
     CDS             complement(732..1805)
                     /locus_tag="VHE37_14250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HVT36747.1"
                     /translation="MKFVTENTRIKSIRAVSTPAQVQEELPLTEGAAKTAYETRREIQ
                     DILHGRDDRLLVIVGPCSIHDTAAALDYAQRLAVSKEQLKKNLLIVMRVYFEKPRTTV
                     GWKGLINDPDLDDSYQIDKGLRVARKLLLDLNERGMPAGVEYLDILTPQYLSDLVSWG
                     AIGARTTESQLHRELASALSCPVGFKNGTDGSVKVAVDAVMSARHPHRFLSLTKDGQS
                     AIFETTGNRDCHIILRGGSLGTNYDAGAVEAACAALAKAKLPQQVMIDFSHANSSKQH
                     KRQVVVGHDVGSQIAGGDKRIIGAMIESHLVEGRQDIAPRDQMTYGQSVTDACIHWDD
                     TAELLRNLAGAVEQRRVQLAKSA"
     gene            1991..2962
                     /locus_tag="VHE37_14255"
     CDS             1991..2962
                     /locus_tag="VHE37_14255"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HVT36748.1"
                     /translation="MQAKTAVCGALALLCALACAPPAQAQEFFPVYNQGSLARAFDLP
                     ALGRPRVLAPDASAWYGAVDLSNEYYTDSSAREFLLEDGETARFSLSYARGIGSNVEV
                     SATLPLLVQGGGFIDHTIEQWHRIFGLPNGGREFAPRQRYQYTYTRDGVTVMDAHESG
                     TRLGDVLLGAGWQWTSSTALRAQLKLPTGDKGQLAGGNAGGALWLDTALPFDRASTLS
                     GYLSGGVSANGSSGPLGDLQQRVLGFGGGGLGWQAFNRVQLVGQLYFHSPLYKDTDIS
                     GLKKPGGQLLLGANVRVAPGTLFKIAFQEDIVTNSSPDFSLHFGLSL"
     gene            complement(2968..3666)
                     /locus_tag="VHE37_14260"
     CDS             complement(2968..3666)
                     /locus_tag="VHE37_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVT36749.1"
                     /translation="MISLRRSDERGHAEHGWLDARHTFSFSDYHDPRWMGYGDLRVIN
                     EDRVAPGAGFAPHGHRDMEIITYMLDGALAHHDSTGGAGVLRPGEVQVMSAGRGVRHS
                     ETNASQRETAHLLQIWIEPRETGVAPAYAQAKLDADALRAGFSTIVAPHGESAPFHIH
                     QDARLSIAWPAAAQTLTRALDTQRRYYLHVARGNVLLGGFTLASGDAAMIEREHALEL
                     RAEQDSELLLFDLA"
     gene            complement(3707..4243)
                     /locus_tag="VHE37_14265"
     CDS             complement(3707..4243)
                     /locus_tag="VHE37_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36750.1"
                     /translation="MPSCSCHPISAHKKLRIPDWYVSSLMLDRALDAIVRRVKQNRSY
                     DIPYLAGYSQDGKTIYIDRHLPRIWTYRRRKIEVDRYLILHEEVEKTLLDRLDLHYLH
                     AHQIATRAEEAAVRADRISWRAYDAFMQKYVKQIGDERLRKVPRDLDLKPYRDEHDLD
                     LINRMQRAQGKAAPLRRR"
     gene            4337..>4717
                     /locus_tag="VHE37_14270"
     CDS             4337..>4717
                     /locus_tag="VHE37_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVT36751.1"
                     /translation="MSRLDTITAKDYMSSELLTFTPDMELMTAINLLAKRRISGAPVL
                     DGAGKVIGILSEKDCLKVCLAAGYEGVPGGLVREYMSSKVVTVEADASLLEVAGLFVN
                     SPYKRLPVMKDGKLAGQISRSDVLR"
BASE COUNT          742 a         1534 c         1598 g          843 t
ORIGIN      
        1 tccgcctgtt cccctcatcc agcgccgcca aggggcgcaa ggaaggtgag aaacccacgc
       61 tggaacaggt ggtgttcgcc aacgtgttca agggcgagca ctggtaggct tcgtcgtatt
      121 gacattgtca atacatggcg gataatcggc gcatggatgg cgagatccgc cacgaatacg
      181 acggcaccac tttcgtttgg cgcgccgcca aggccgcggc caacctggcc aagcacggcg
      241 tcagcttcga cgaagccacg acggtgtttt ccgatccgct gctgcgcctg gtcgacgcca
      301 gccgcaacga cgaggcgcgc gatgcggtga ttgggttcga tctgcaggcg cggctgctgt
      361 tcgtggttca tgtggagatc gacggcgaag ccattcgcct gatctccgcg cggcgggcct
      421 cgcgcgacga ggaggactta tatgctcaat gacaaactca agcagcgcct gcgccgcgac
      481 cggccttcag tcagcatcac gctgcgcatt ccgcaggacg tggtggacac gctgaaggaa
      541 ctggcaccta ggctcggcat gtcgggctac cagaccctgc tcaagagcta catcagcgag
      601 ggcttgcgcc gcgacgaagc gcgcatggtc ggcgtcagcg gccagaagct gattgcggcg
      661 ttgcgccggc atggcgtatc cgccaaggta ctcgacgccg cgctgcacga cctcgaaaaa
      721 gcggcgtagc gctacgccga tttcgccagc tgcacccgcc gctgctccac tgcacccgcc
      781 aggttgcgca gcagctcggc ggtgtcgtcc cagtggatgc aggcgtcggt gacgctctgg
      841 ccgtaggtca tctggtcgcg cggcgcgatg tcctggcggc cttccaccag gtgcgattcg
      901 atcatggcgc cgatgatgcg cttgtcgccg ccggcgatct ggctgccgac gtcgtggccc
      961 accaccacct gccgcttgtg ctgcttgctg ctgttggcgt ggctgaaatc gatcatcacc
     1021 tgctgcggca gtttcgcttt cgccagcgcc gcgcatgcgg cctcgaccgc gcccgcgtcg
     1081 tagttggtgc cgagcgagcc gccgcgcagg atgatgtggc aatcgcggtt gccggtggtc
     1141 tcgaaaatgg ctgactggcc gtccttggtc agcgacagga aacgatgcgg atggcgcgcc
     1201 gacatcaccg cgtccaccgc caccttcacg ctgccgtcgg tgccgttctt gaagccgacc
     1261 gggcacgaca gcgcggaggc gagctcgcgg tgcaactgcg attccgtggt gcgcgcgccg
     1321 atcgcgcccc agctcaccag gtcggagaga tattgcggcg tgaggatgtc gaggtactcc
     1381 acgcccgccg gcatgccgcg ctcgttgaga tcaagcagca gcttgcgcgc cacgcgcagg
     1441 cccttgtcga tctggtagct gtcgtcgaga tcggggtcgt tgatcaggcc cttccagccc
     1501 acggtggtac gcggcttctc gaagtacacg cgcatcacga tcagcaggtt cttcttcagc
     1561 tgttccttcg acacggccag ccgctgcgcg tagtcgagtg cggcggcggt gtcgtgaatc
     1621 gagcacgggc cgacgatgac cagcaggcgg tcgtcgcggc cgtgcaggat gtcctggatc
     1681 tcgcgccgcg tttcgtacgc cgtcttcgcc gcgccttcgg tgagcggcaa ttcttcctgc
     1741 acctgcgccg gcgtggacac ggcgcggatc gacttgatgc gggtgttttc ggtgacgaac
     1801 ttcatgacgc ttccaacggt gctgcgaagt ggggcgcgga tgctaacaga gcgccatgat
     1861 cttcgttaag ctccgtttgg cctctgcacc ggcgattttt cctctcaccg cactgctgtt
     1921 ctggatgtta taaataatca tgcaaaataa aaataatacg ttatttaata acatcgccag
     1981 ggccggtgcg gtgcaggcaa aaacagccgt ctgcggcgcg ctggcgttgc tctgcgcgct
     2041 ggcctgcgcg ccgcccgcgc aggcgcagga attctttccg gtctacaacc agggttcgct
     2101 ggcgcgcgcg ttcgacctgc ccgcgctcgg ccgtccgcgc gtgctcgcgc cggatgccag
     2161 cgcgtggtac ggcgcggtgg acctgtcgaa cgagtactac accgacagca gcgcgcgcga
     2221 gttcctgctc gaggacggcg agaccgcgcg cttctcgctc agctatgcgc gcggcatcgg
     2281 cagcaatgtc gaagtctccg ccacgctgcc gctgctggtg cagggcggcg gctttatcga
     2341 tcacaccatc gagcaatggc accgcatctt cggcctgccc aacggcggcc gcgagttcgc
     2401 gccgcgccag cgctatcaat acacctacac gcgcgacggc gtgaccgtga tggatgcgca
     2461 cgagagcggc acgcggctgg gcgacgtgct gctcggcgcc ggctggcaat ggacgtcgtc
     2521 gaccgcgctg cgcgcgcaat tgaaattgcc caccggcgac aagggtcagc tggcgggcgg
     2581 caatgccggc ggcgcgttgt ggctggacac cgcgctgccg ttcgaccgcg catcgacgct
     2641 gagcggctat ctctccggcg gcgtctccgc caacggcagc tcaggcccgc tgggcgacct
     2701 gcagcagcgc gtgctcggct tcggcggagg cgggctgggc tggcaggcgt tcaaccgcgt
     2761 gcagctggtt ggccagctct acttccactc gccgctgtac aaggacaccg acatcagcgg
     2821 actgaaaaag cccggcggcc agctgctgct gggcgccaac gtgcgcgtgg cgccaggcac
     2881 gctgttcaag atcgcgttcc aggaagacat cgtcaccaac tcgtcgccgg atttctcgct
     2941 gcacttcggc ctgagcctct gagcaaatca cgccaggtcg aacagcagca gctcgctgtc
     3001 ctgctccgcg cgcagttcca gcgcgtgctc gcgctcgatc atcgccgcgt cgccgctggc
     3061 cagcgtgaag ccgccgagca ggacatttcc gcgtgccacg tgcaggtagt agcggcgctg
     3121 cgtgtcgagc gcacgcgtca gcgtctgcgc cgccgccggc caggcgatgg acagccgcgc
     3181 atcctggtgg atatggaacg gcgcgctttc gccgtgcggc gccacgatgg tgctgaagcc
     3241 tgcgcgcagc gcgtccgcgt ccagcttcgc ctgggcatag gccggcgcca cgccggtctc
     3301 gcgcggctcg atccagatct gcagcaggtg cgcggtctcg cgctgcgagg cattggtctc
     3361 gctgtggcgc acgccgcggc cggcgctcat cacctgcact tcgcccggcc gcagcacgcc
     3421 ggcgccgccg gtggagtcgt ggtgcgccag ggcgccatcg agcatgtagg tgatgatctc
     3481 catgtcgcgg tggccgtgcg gtgcgaagcc ggcgccgggc gccacgcggt cttcgttgat
     3541 cacgcgcaga tcgccgtagc ccatccatcg cggatcgtgg tagtcggaaa atgagaaggt
     3601 gtggcgcgca tcgagccagc catgttcggc gtggccgcgc tcgtcggacc ggcgcaggct
     3661 gatcatcggc attctccgtg cgggcaagcc cgcaagatgg ggccggtcag cgccggcgca
     3721 agggcgcggc cttgccctgc gcgcgctgca tgcggttgat caggtcgagg tcgtgctcgt
     3781 cgcggtacgg tttcaggtcc aggtcgcgcg gcaccttgcg caggcgctcg tcgccgatct
     3841 gcttgacgta cttctgcatg aaggcgtcgt aggcgcgcca ggaaatccgg tcggcgcgca
     3901 ccgccgcttc ctccgcgcgc gtggcgatct ggtgcgcgtg caggtagtgc aggtccaggc
     3961 ggtcgagcag ggtcttctcc acctcctcgt gcaggatcag gtagcggtcc acctcgatct
     4021 tgcgtcggcg gtaggtccag atgcgcggca ggtggcgatc gatgtagatg gtcttgccgt
     4081 cctggctgta gccggccagg tacggaatgt cgtagctgcg gttctgcttc acccggcgca
     4141 cgatggcgtc aagcgcgcgg tccagcatca ggctggacac gtaccagtcg ggaatgcgca
     4201 gttttttgtg cgcggaaatc gggtgacagc tgcagctggg catgcggacc tcgcgggtgc
     4261 ttgcgcgcaa acgcggcgcg caccatcatg aggccatccg gcgcggcttt acaaaacttt
     4321 gctcggagga gatcacatgt cgagactcga caccatcacg gccaaggact acatgagcag
     4381 cgagctgctc accttcacgc ccgacatgga gctgatgacg gcgatcaacc tgctggccaa
     4441 gcgccgcatc tccggcgcgc cggtgctcga cggcgccggc aaggtgatcg gcatcctgtc
     4501 ggagaaggac tgcctcaagg tgtgcctcgc cgccggctac gagggcgtgc ccggcgggct
     4561 ggtgcgcgaa tacatgagca gcaaggtggt gacggtggaa gccgacgcct cgctgctgga
     4621 agtggccggc ctgttcgtca actcgccgta caagcgcctg ccggtgatga aggacggcaa
     4681 gctggcgggc cagatttcgc gttcggacgt gctgcgc
//
LOCUS       DASZZC010000672         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_626268,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000672 DASZZC010000000
VERSION     DASZZC010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626268"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VHE37_14275"
     CDS             complement(<1..761)
                     /locus_tag="VHE37_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36752.1"
                     /translation="MKKLPASSFVPALAAGVLTLLVMPHYAPAPARVAAHDDGCPAGY
                     EMRDPLAAAREFNERYAEAHENEIRAKFGTQLCMSKLLMPEAMSEAAERQNDAREILG
                     PVPNDAPRRAVEQKAVLQAKAASVPNSGGHWVAYGMAPQVQDDAYPMGSADGITQVSG
                     RTDNFAYDADHHRLFAAVGNGGIWYSEAPDGDVSTLGDHWTSIGDHLPTQINSAVAWT
                     SAAGGRVLVLSGENTQGGNAYVGLGAYWSDDLGQN"
     gene            complement(791..3709)
                     /locus_tag="VHE37_14280"
     CDS             complement(791..3709)
                     /locus_tag="VHE37_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36753.1"
                     /translation="MNRLNEVARRRAAWPAALALAALSCMLYGWMPAPHACAQPRDGD
                     GEDADTCMHAHSPEPLREFLGLRQQFLAQRAGGHGSLRAALAQRDALKSAQEKIANAG
                     GDWSVYGTPKLIVNDPRYGSVNGDGYVYNSGRIDSFDYDAAGKRLFASLGTGGIWMSA
                     DKGQHWTSVGDALPSQSVGSVAWSAAGGGTIVVAGGDPSFGGDDYAGIGAFWSNDLGQ
                     SWHIADGVPEGILGFKAAVDHAHPDIVYLGTSKGLFRSTDAGRSYVNVALPTSADCAG
                     VTDIGSKCQYANYVTDVIVKEPGGTTNEAGGQVLAGVGYRAGQLALSDGTISAPGNGL
                     YYSGSGAPGSFSKLSNSAANSLSPLGFTVQERIGRIAFGEALGDAQDHNYVYAMVQDA
                     VLFNHGFPLLDLPEGLGTAITGALPVGLSSTLFNGLYVSADFGQTWTRAADTEEIANT
                     PTSALAATGAATNYAPGVQGYYNLWVKPDPTAQLSGVPTRLAFGLEEVFSNLVNDPQN
                     GTLQVGPSDYQVVGAYRTSTAPGTILTGGTTTHPDQHAAVWIPDDDGSGGVTLVVGND
                     GGAYSQHIGATGGLQQSGWGDGIQDGFNTLLPYSVAVAKDGVAWFGLQDNGSGKVDTD
                     GKYYENYGGDGFYVAVDPDDSNTAWEEYTMADMRYTTDGGQSWTSNAPSLTGANFANF
                     FMMDPTDATHMMTAAKEVMEMTAGPSGSWTQVFDLGTSSNAGGANNIMSALDLQGDNA
                     YVGFCGVCDILNNQAAGFHNGIATNVNGDKPPQRGTSDGWHFAAAGGLPNRYISGVEI
                     DPADPRTVYVTLGGYANRGWEPPGSYLDANPDIGSGHVFVSHDAGEHFSDISGNLPDV
                     HATAILLRQGQLIVGTDIGAFISSDVGGSSWAPLGNALPAMPILQFQLMPGDDSKLVA
                     ATFGRAIWTYTFGAGGAAAAPAANAAAARSRFGGALGAPLLAVLLLGGLWRRRAHRWN
                     "
     gene            complement(3895..>4338)
                     /locus_tag="VHE37_14285"
     CDS             complement(3895..>4338)
                     /locus_tag="VHE37_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVT36754.1"
                     /translation="GTADEYLKALGADQIVSRQSVDLGKKPLEAGLWGGAVDNLGGET
                     LAWLTRTVKPWGNIASIGLAQSHELATTVMPFILRGVSLLGIHSVECPRAWREDIWKH
                     LAGDWKPRHLERIATREIALDEVQRYCDEVIAGKVTGRAIVRVGG"
BASE COUNT          663 a         1472 c         1433 g          770 t
ORIGIN      
        1 cagttctggc ccaggtcatc gctccagtac gcgccgagac cgacataggc gttgccgccc
       61 tgcgtgttct cgcccgacag caccagcacg cgcccgccgg cagcggaggt ccatgccacc
      121 gcgctgttga tctgcgtcgg caggtgatcg ccgatggaag tccagtggtc accgagcgtg
      181 gagacatcgc catccggcgc ctcgctgtac cagatgccgc cgttgccgac cgcagcgaac
      241 aggcgatggt gatcggcgtc gtaagcgaag ttgtcggtgc gcccggacac ctgcgtgatg
      301 ccgtcggccg agcccatcgg gtatgcatcg tcctgcacct gcggcgccat gccgtaggcg
      361 acccagtgtc cgccggagtt gggcacgctg gcggccttgg cctgcaacac ggccttctgt
      421 tccaccgcgc ggcgcggcgc atcgttgggc accgggccga gaatttcgcg cgcatcgttc
      481 tggcgctcgg ccgcttcgct catcgcttcc ggcatcagca gtttcgacat gcacaactgc
      541 gtcccgaatt tcgcgcggat ctcgttctcg tgcgcctcgg catagcgctc gttgaactcg
      601 cgcgcggcgg cgagcggatc gcgcatctcg tagccggcag ggcaaccgtc gtcgtgcgca
      661 gccacgcgcg caggcgcggg tgcgtagtgc ggcatgacca gcaaggtgag cacgccggcg
      721 gcgagcgccg gaacgaatga cgaggcaggc agcttcttca tggcaacgat tccttcgatg
      781 aacccggcaa tcaattccag cgatgagccc ggcggcgcca taggccgccg agcagcagca
      841 cggcgagcag cggtgcgccc agcgcgccgc cgaagcggct gcgcgcagcc gcggcgttcg
      901 ccgccggcgc ggcggcagcg ccgccggcac caaacgtgta ggtccagatg gcgcgcccga
      961 aagttgcggc gaccagcttg ctgtcgtcgc cgggcatcag ctggaactgc aggatcggca
     1021 tcgccggcaa cgcgttgccc agcggcgccc agctgctgcc gccgacgtcg ctggaaatga
     1081 acgcgccgat gtcggtgccg acgatcagct gcccctggcg cagcaggatg gcggtggcgt
     1141 gcacgtccgg caggttgccg ctgatgtcgc tgaagtgctc gccggcatcg tgcgaaacga
     1201 atacgtgccc gctgccgata tcgggattgg catcgaggta cgaacccggc ggttcccagc
     1261 cgcggttggc gtagccgccg agcgtcacgt acaccgtacg cggatcggcg gggtcgatct
     1321 ccaccccgct gatgtagcgg ttcggcaggc cgcccgcggc ggcgaaatgc cagccgtcgg
     1381 acgtgccgcg ctgcggcggc ttgtcgccat tcacgttcgt ggcgatgccg ttgtgaaagc
     1441 ccgccgcctg gttgttgagg atgtcgcaca cgccgcagaa accgacataa gcgttgtcgc
     1501 cctgcaagtc gagcgccgac atgatgttgt tggcgccgcc ggcattgctg ctggtgccga
     1561 ggtcgaacac ctgcgtccag ctgccgctgg gacccgcggt catctccatc acttccttcg
     1621 ccgccgtcat catgtgcgtg gcgtcggtcg gatccatcat gaagaaattg gcgaagttgg
     1681 cgccggtgag cgacggcgca ttgctggtcc agctctggcc gccgtcggtg gtgtagcgca
     1741 tgtcggccat ggtgtactcc tcccaggccg tgttgctgtc gtcgggatcg accgccacgt
     1801 agaagccgtc gccgccatag ttctcgtagt acttgccgtc ggtatcgacc ttgccgctgc
     1861 cgttgtcctg caggccgaac cacgccacgc cgtccttggc caccgcgacg ctgtacggca
     1921 gcagcgtgtt gaagccgtcc tggataccat caccccatcc cgattgctgc aatccgccag
     1981 tcgcgccgat gtgctggctg taagcgccgc catcgttgcc caccaccagc gtcacgccgc
     2041 cgctgccgtc atcatcgggt atccataccg ccgcgtgctg gtcgggatgc gtggtggtgc
     2101 cgccggtgag gatggtgccg ggcgcggtgc tggtgcggta ggcgcccacc acctggtagt
     2161 ccgacggccc gacctgcagc gtgccgttct gcggatcgtt caccaggttg gagaacactt
     2221 cttccaggcc gaaagcgaga cgtgtcggaa cgcctgagag ctgcgcggtg ggatctggct
     2281 tgacccacag gttgtagtaa ccctgcacgc cgggcgcgta attcgtcgcc gcgccggtgg
     2341 ccgccagcgc ggaggtcggc gtgttggcga tttcctcggt gtcggccgcg cgcgtccagg
     2401 tctggccgaa atcggcggag acatacaggc cgttgaacag cgtgctcgac aatccgaccg
     2461 gcagtgcgcc ggtgatcgcc gtgcccaggc cttccggcag atcgagcaag gggaatccgt
     2521 ggttgaacag caccgcgtcc tgcaccatcg cgtacacgta gttgtgatcc tgcgcgtcgc
     2581 ccagtgcttc gccgaaggcg atgcggccga tgcgctcctg cacggtaaag ccgagcggcg
     2641 acagcgagtt cgccgcggag ttgctcagct tgctgaagct gcccggcgcg ccgctgcccg
     2701 agtagtacag gccattgccc ggcgcagaga tggtgccgtc ggacagggcg agttgtccgg
     2761 cgcggtagcc gacgccggcg agcacctgtc cgcccgcctc gttagtggtg ccgcccggct
     2821 ccttgacgat gacgtcggtc acgtaattgg cgtattggca cttgctgccg atgtcggtga
     2881 cgccggcgca atccgcggag gtcggcagcg cgacgttcac gtaactgcga ccggcgtcgg
     2941 tcgaacggaa cagtcccttg ctggtgccca agtagacgat gtcgggatgc gcgtgatcga
     3001 ccgcggcctt gaagccgaga atgccctcgg gcacgccgtc ggcgatatgc cagctctggc
     3061 ccaggtcatt gctccagaac gcgccgatgc cggcgtagtc gtcgccgcca aaggaaggat
     3121 cgccgccggc caccacgatc gtgccgccgc ccgccgcgct ccacgccacc gagccgaccg
     3181 actgcgacgg cagcgcgtcg cccaccgaag tccagtgctg gcccttatcg gcgctcatcc
     3241 agatgccgcc ggtgcccagg ctggcgaaca gtctcttgcc cgccgcgtcg tagtcgaagc
     3301 tgtcgatgcg cccgctgttg tacacgtaac cgtcgccgtt gacgctgccg tagcgcggat
     3361 cgttgacgat cagcttgggc gtgccataca ccgaccagtc gccgccggcg ttggcgattt
     3421 tttcctgcgc ggacttgagt gcgtcgcgct gcgccagcgc cgcgcgcagc gagccatggc
     3481 cgcccgcgcg ctgggcgagg aattgctggc gcagtccgag gaattcacgc aagggctcgg
     3541 gcgagtgcgc gtgcatgcag gtgtcggcgt cttcgccatc gccatcgcgc ggctgtgcac
     3601 acgcgtgcgg cgcaggcatc catccgtaca gcatgcagct gagcgccgcc agcgccagcg
     3661 cggccggcca tgcggcacgc ctgcgcgcca cctcgttcaa cctgttcatg cgctccctct
     3721 ctcccgttag cgcgatgctg gtgcagcacc ggctgccacg caaccgtttt cgatggcaaa
     3781 tccgctccac cgcaccggca ttttgtttgt tattaaatat aatattattt taaataaaaa
     3841 ctatttttta taacatcgta ccggcccttg cggtataggc aattttcctg ccgatcagcc
     3901 gcccacgcgc acgatcgcgc gcccggtgac cttgccggcg atcacctcat cgcagtagcg
     3961 ctgcacttca tccagtgcga tctcgcgcgt ggcgatgcgc tcgagatggc gcggcttcca
     4021 gtcgccggcc aggtgcttcc agatgtcctc gcgccaggcg cgcgggcatt ccaccgagtg
     4081 gatgcccagc agcgagacgc cgcgcaggat gaacggcatg accgtggtcg ccagctcgtg
     4141 cgactgcgcc aggccgatcg atgcgatgtt gccccagggc ttcaccgtgc gcgtgagcca
     4201 ggccagcgtc tcgccgccga ggttgtcgac cgcgccgccc cacagcccgg cctcgagcgg
     4261 cttcttgccc aggtcgaccg attgccgcga cacgatctgg tcggcgccga gtgctttcag
     4321 atattcgtct gctgtgcc
//
LOCUS       DASZZC010000673         5912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1270131,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000673 DASZZC010000000
VERSION     DASZZC010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270131"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHE37_14290"
     CDS             complement(<1..193)
                     /locus_tag="VHE37_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36755.1"
                     /translation="MSTLLIMAPHAGLWPPWLRGIFPEIAAVGAGEVSCHALVVINDH
                     SAAALGLLRRALELRAARPH"
     gene            complement(190..795)
                     /locus_tag="VHE37_14295"
     CDS             complement(190..795)
                     /locus_tag="VHE37_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HVT36756.1"
                     /translation="MVQVISGSLDDAAFARGPAEQMFRLRGQIFGGRLGWDVQVRDGC
                     ETDGYDDAHSRYLLLAEGDTVIGGWRLRPTTQPYMLDEVFPQLLGGRRAPHHPRVWEV
                     SRFAIDTRGDRSARFGFNQAARSLLAATAQYALDHGIERYVMVASAGAERLYRNAGLQ
                     VRRYGPPQRIGRVLSVAGWIDIDAHTCHVLLGHPLPLARAA"
     gene            complement(912..1697)
                     /locus_tag="VHE37_14300"
     CDS             complement(912..1697)
                     /locus_tag="VHE37_14300"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HVT36757.1"
                     /translation="MEATRRDGSVDGQIETARNIQQVGAACRRAAETLGYRYFQFGFR
                     VPVSLTRPCQIVLSGYPQEWRTWYDDCGYLTIDPVVQRALGSVLPFGWDELALETPEA
                     QRLFAEAARYGLAHGFSVPVHGAHGESALFSFARGEALPAGAVRAEQFRHAHWLTALI
                     HERLRTLVYEGEGIGRLHRRLTRRERQCLQLAAEGLSATAIGRGMKISEHTVNFHLVN
                     AEAKLGARSRRHAVARAVALGEIEPGCYPPQLRQSQELVDLRV"
     gene            complement(1718..2134)
                     /locus_tag="VHE37_14305"
     CDS             complement(1718..2134)
                     /locus_tag="VHE37_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36758.1"
                     /translation="MDWKPPEWNALSSRELAAVPPVKVAVGLIGGAPKLAKLRSKTVS
                     LATAAVPAKLQLAVWIAQRHYPVQRKTDLEQSVRASLARPYAVENMVYLLNQQDGPWA
                     TAISGHDISWKPVKHEEVACLLTRGEFLELVFHHLR"
     gene            2249..3016
                     /locus_tag="VHE37_14310"
     CDS             2249..3016
                     /locus_tag="VHE37_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HVT36759.1"
                     /translation="MSSAQHLERHLSKHDLVGILDVIDAAISVGSEAQFRHLMQLTAQ
                     IVPIERAHVSVADLDGTRAVVRTSRQLNLNFPDGWLSTYRQEHLHAIDPVARVLFTAN
                     RPLIWSQVRERDRSRAARAFYGQAAEFGLRNGFSFGAPFAKSPSASFFSCAGADLTHT
                     PRHVLALQYLVPHLHAALSKMHLGMLKEAPGLTPREIEVLNWAKFGKTNWEISLQLNT
                     SQRVVKFHIENAIRKLHANNRSQAVAIALSQGLIEWG"
     gene            3131..3823
                     /locus_tag="VHE37_14315"
     CDS             3131..3823
                     /locus_tag="VHE37_14315"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HVT36760.1"
                     /translation="MDRFGQLVEATRADHAQHILKNPLIRKLAAGEVTHDLYAVYLGQ
                     TYQLVRHTSRALALAAARLPDERRALRAWFLEQAQEEHGHELFCITDLKNLGLDPARV
                     LECGPLPGAWGLYTQNYYMADHGNPAAILGVATATEGMGAELAGGMAQLLVERYGVPA
                     TAVTFLRSHAGFDQRHLEQARRAINELALETDLPGILHARRMTFRYYGRLFEDVSEST
                     ERYFFRSTLAAA"
     gene            3853..4524
                     /locus_tag="VHE37_14320"
     CDS             3853..4524
                     /locus_tag="VHE37_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36761.1"
                     /translation="MSTAGDPALAAFRPECFHTRRIGGGNDLAQYQRLLEQTYEPLGF
                     MVSAVLPTAASMQYKVTHCGRIVAIFRLTPVDDGDSAYFHLVPGACADGRPRRLLEVN
                     NVVIASPFRATILLGLLLYESAKAAHRLGYDYVVGITRHQALKFFVDFGVVPVDHPPL
                     RLLGKEHLLDFVIYYDTRSPESIGYMHERARRWFHQQYVLRSIHRKYVEPLRAPAAPA
                     LAVNA"
     gene            4532..5572
                     /locus_tag="VHE37_14325"
     CDS             4532..5572
                     /locus_tag="VHE37_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HVT36762.1"
                     /translation="MPALIYPAGLLLALHLGGAGCWLLVPFNLLLVPLADQLLDAVRW
                     QPSPRLLRSCFAPWVYWIYAAAQLGALLAALHAICLRNLSLLEWMGLVSSTGLMTGTA
                     GITAAHELIHRTSPAQRGLGLALLAMTSYMHFRIEHVYGHHRFAGTARDAASARLGQS
                     FPGFFLRALLHGGRDAWRIERARLARAGRSAWSPRNRLLHYLLLQGAVYAAAAWFGGW
                     MAPLFLLLQSLMAVHLLEAVNYVQHYGLRREAGAGGEPRRMRAADSWDSTSPLAPLLS
                     FNIVRHAQHHLSVRERAHQLPVLAGSPKLPASFFAMVFCALIPPLWHRLMDPRAQAPE
                     RYTHTDPRPLTS"
     gene            5569..>5912
                     /locus_tag="VHE37_14330"
     CDS             5569..>5912
                     /locus_tag="VHE37_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36763.1"
                     /translation="MNATLPVADAGTAASAAPTLAPWPLRALLRTAGTLLPHATGQFL
                     ARRMLTPRRRPVRPQAWIAAAAALKIPAEGGMVNAYRWGDGGATVLLMHGWEGGVDDF
                     AALVPQLAGAGFS"
BASE COUNT          853 a         2164 c         1976 g          919 t
ORIGIN      
        1 ggtgcggccg cgccgcgcgc agttccagcg cgcgccgcaa caagcccagt gcggcggcgc
       61 tgtggtcatt gatcaccacc agcgcatggc agctcacctc gccggcgccc accgcggcga
      121 tctccggaaa gatgccgcgc agccacggcg gccacaggcc ggcgtgcggc gccatgatca
      181 gcagcgtgct catgcggccc gcgccagcgg cagcggatgg ccgagcagca cgtggcaggt
      241 gtgcgcgtcg atgtcgatcc agccggccac cgacaacacg cggccgatgc gctgcggcgg
      301 gccgtagcgc cgcacctgca ggccggcgtt gcggtacagg cgctcggcgc cggcgctggc
      361 caccatcaca tagcgctcga tgccgtgatc cagcgcgtat tgcgcagtgg ctgcaagcag
      421 gctgcgtgcg gcctggttga agccgaagcg ggcgctgcgg tcgccgcgcg tgtcgatggc
      481 gaagcggctg acctcccaca cgcgcggatg atgcggcgcg cggcggccgc cgagcagttg
      541 cgggaacact tcgtcgagca tgtacggctg cgtggtcggg cgcaggcgcc agccgccgat
      601 caccgtgtcg ccctcggcga gcagcaggta gcggctgtgc gcatcgtcgt agccgtcggt
      661 ttcgcagccg tcgcgcacct gcacgtccca gcccagccgc ccgccgaaga tctgtccgcg
      721 caggcggaac atctgctcag ccggtccgcg cgcgaacgcg gcgtcgtcga ggctgccgct
      781 gatgacctgg accatggcgt ctcctgcacc gatgagggag acgcgatatc aacatccgcg
      841 gcgacaggat cgccccgtgg aattccacgg gttgcgccga agtcgcctga gtcagcggga
      901 aatttttccg ctcagacgcg caggtccacc agttcctgcg actgccgcag ctgcggcggg
      961 tagcagcccg gctcgatctc gcccagggcc actgcgcgcg ccaccgcgtg gcggcgcgaa
     1021 cgcgcgccga gcttggcctc ggcattgacc aggtggaagt tcaccgtgtg ttcgctgatc
     1081 ttcatgccgc ggccgatggc ggtggcgctg agcccctcgg ccgcgagctg caggcactgg
     1141 cgctcacgcc gcgtcagccg gcgatgcagc cggccgatgc cctcgccctc atacaccagc
     1201 gtgcgcaggc gctcgtggat cagcgcggtg agccagtgcg cgtggcggaa ctgctcggcg
     1261 cgcaccgcgc ccgcgggcaa cgcttcgccg cgggcgaagc tgaacagcgc gctctcgcca
     1321 tgcgcgccgt gcaccggcac cgagaagccg tgcgccaggc cgtagcgcgc ggcctcggcg
     1381 aacaggcgct gcgcctccgg cgtctccagc gccagttcgt cccagccgaa cggcagcacg
     1441 ctgcccagcg cgcgctgcac caccgggtcg atggtgagat agccgcagtc gtcgtaccag
     1501 gtgcgccact cctgcggata gccggacagc acgatctggc acggccgggt cagcgacacc
     1561 ggcacgcgga agccgaactg gaaatagcgg tagccgagcg tctcggcggc gcggcggcag
     1621 gcggcgccca cctgctggat gttgcgcgcc gtctcgattt gcccgtcgac cgagccgtcg
     1681 cgccgcgtcg cttccattgc cccgctcccc tgcacactca gcgcagatga tgaaatacca
     1741 gttcgaggaa ttcgccgcgc gtgagcaggc aggcgacctc ttcgtgcttc accggcttcc
     1801 acgagatgtc gtggccggaa atggccgtgg cccacggccc gtcctgctgg ttgagcaggt
     1861 acaccatgtt ctccaccgcg taaggccgcg ccagcgaagc gcgcaccgac tgttccaggt
     1921 cggtcttgcg ctgcaccggg taatggcgct gtgcgatcca caccgccagt tgcagctttg
     1981 ccggcactgc tgccgtggcc aggctcacgg tcttgctgcg cagcttggcc agcttgggcg
     2041 cgccgccgat caggccgacg gcaaccttca ccggcggcac cgctgccagt tcacgcgagc
     2101 tcaaggcatt ccattccggc ggtttccagt ccattgcgtc acgctcctgc gctgctgttt
     2161 ccaccgccgc caagttagcg gctggaccag gcgctggctg cctgtccaat cggacagcgg
     2221 ccaggcgctt ccgctaggct agggcgccat gagcagcgcg cagcatctcg agcggcatct
     2281 gtccaagcac gacctggtcg gcatcctcga cgtgatcgac gccgccatct cggtgggcag
     2341 cgaggcgcag ttccgccacc tgatgcagct gaccgcgcag atcgtgccga tcgagcgcgc
     2401 ccacgtcagc gtggccgatc tcgacggaac ccgggcggtg gtgcgcacca gccgccagct
     2461 gaatctcaat tttcccgacg gctggctcag cacctaccgg caggaacatc tccatgccat
     2521 cgatccggtg gcgcgcgtgc tgttcaccgc caaccggccg ctgatctggt cgcaggtgcg
     2581 cgagcgcgac cgcagccgcg ccgcacgcgc gttctacggc caggcggccg agttcgggct
     2641 gcgcaacggc ttctccttcg gcgcgccctt cgccaagtcg ccgtcggcca gcttcttctc
     2701 ctgcgccggc gccgacctca cccacacgcc gcgccacgtg ctggcgctgc agtacctggt
     2761 gccgcacctg cacgccgcgc tgtcgaagat gcacctgggc atgctcaagg aggcgccggg
     2821 cctgaccccg cgcgagatcg aggtgctgaa ctgggccaag ttcggcaaga ccaactggga
     2881 gatttcgctg cagctcaaca ccagccagcg cgtggtcaag ttccacatcg agaacgccat
     2941 ccgcaagctg cacgccaaca accgctcgca ggcggtggcc atcgcgctgt cacaggggct
     3001 gatcgaatgg ggctgatgcc actgcgcgcg agcggctgac ggtcgtcctg tccgaacgga
     3061 caggagcggc gccgacgttc gccgcgcaat gattcctcca cgatgtgcat cgagaccacg
     3121 gaggaatcac atggatcgct ttggccagct ggtggaggca acgcgcgcag atcatgcaca
     3181 gcacatcctg aagaatccgc tgatccgcaa gctggccgcg ggcgaggtca cgcacgatct
     3241 ctacgccgtg tacctgggcc agacctacca gctggtgcgc cacacttcgc gcgcactggc
     3301 gctggcggcg gcgcgcctgc ccgacgagcg ccgcgcactg cgcgcctggt tcctcgagca
     3361 ggcgcaggag gagcacggcc acgaactgtt ctgcatcacc gacctgaaga acctggggct
     3421 cgacccggcg cgcgtgctcg aatgcggtcc gctgccgggc gcctggggcc tgtacacgca
     3481 gaactactac atggcggatc acggcaatcc ggcggccatc ctcggcgtgg ccaccgccac
     3541 cgagggcatg ggcgccgagc tggccggcgg catggcgcag ctgctggtcg agcgctacgg
     3601 cgtgcctgcc accgcggtca ccttcctgcg cagccacgcc ggtttcgacc agcgccacct
     3661 ggagcaggcg cgccgcgcca tcaacgagct ggcgctggaa accgacctgc ccggcatcct
     3721 gcatgcgcgg cgcatgacct tccgctacta cggccgcctg ttcgaggacg tgagcgaatc
     3781 caccgagcgc tactttttcc gtagcacgct ggccgcggcc tgaaccattc cggtcgcacc
     3841 gcgcctatgg ccatgtccac tgcgggagat cctgcgctgg cggcgttccg cccggaatgc
     3901 ttccacaccc gccgcatcgg cggcggcaac gacctggccc agtaccagcg cctgctcgag
     3961 cagacctatg agccgctcgg cttcatggtc agcgcggtgc taccgaccgc cgcctcgatg
     4021 cagtacaaag tcacccactg cggccgcatc gtcgccattt tccgcctgac gccggtggac
     4081 gacggcgaca gcgcctactt tcacctggtg ccgggcgcct gcgccgacgg ccgcccccgc
     4141 cggctgctgg aagtgaacaa cgtggtcatc gccagcccgt tccgcgccac catcctgctc
     4201 ggcctgctgc tgtacgaaag cgccaaggcc gcgcaccgcc tgggctacga ctacgtggtc
     4261 ggcatcacgc gccaccaggc gctgaagttc ttcgtcgact tcggcgtggt gccggtggac
     4321 catccaccgc tgcgcctgct gggcaaggag catctgctcg acttcgtcat ctactacgac
     4381 acgcgctcgc ccgagtcgat cggctacatg cacgagcgcg cgcggcgctg gtttcaccag
     4441 cagtacgtgc tgcgcagcat ccaccgcaag tacgtcgagc cgctgcgcgc gcctgccgcg
     4501 ccggcgctgg cggtcaacgc gtgagcgcag catgccggcc ctgatctatc ccgccggcct
     4561 gctgctggcg ctgcacctgg gtggcgccgg ctgctggctg ctggtgccgt tcaacctgct
     4621 gctggtgccg ctggccgacc agctgctcga tgcagtgcgc tggcagcctt cgccgcgtct
     4681 gctgcgcagc tgcttcgcgc cgtgggtgta ctggatttac gccgcggccc agctcggcgc
     4741 cctgctggcc gcgctgcatg ccatctgcct gcggaatctt tcgcttctgg agtggatggg
     4801 gctggtgtcg tccactggcc tgatgaccgg caccgccggc atcaccgccg cgcacgagct
     4861 gatccatcgc acttcgccgg cgcagcgcgg actgggcctg gcgctgctgg cgatgaccag
     4921 ttacatgcat ttccgcatcg agcacgtgta cgggcatcac cgctttgccg gcaccgcgcg
     4981 cgacgcggcc agtgcgcgcc tgggccaaag cttccccgga ttcttcctgc gcgcgctgct
     5041 gcacggcggc cgcgatgcct ggcgcatcga gcgcgcgcgc ctggcgcgtg ccgggcgcag
     5101 cgcctggtcg ccgcgcaacc gcctgctgca ttacctgctg ctgcagggcg cggtctacgc
     5161 ggccgccgcc tggttcggcg gatggatggc gccgctgttc ctgctgctgc agagcctgat
     5221 ggcggtgcac ctgctggaag cggtcaacta cgtgcagcac tacggcctgc gccgcgaggc
     5281 cggggccggc ggcgagccgc gccgcatgcg cgcggccgat tcgtgggact ccacctcgcc
     5341 gctggcgccg ctgctcagct tcaacatcgt gcgccatgcg cagcatcacc tgtcggtgcg
     5401 cgaacgcgcg caccagctgc cggtgctggc gggctcgccc aagctgccgg ccagtttctt
     5461 cgccatggtg ttctgcgcgc tgattccgcc gctgtggcac cggctgatgg acccgcgcgc
     5521 gcaggcgccg gagcgataca cccacactga cccgaggccg ctgacatcat gaatgccacc
     5581 ctgcccgttg ccgatgccgg gaccgcggct tccgcggcac cgacgctcgc cccctggccg
     5641 ctgcgcgcgc tgctgcgcac cgccggcacg ctgctgccgc atgccaccgg gcaatttctt
     5701 gcgcggcgca tgctcacccc gcgccgcagg cccgtgcgcc cgcaggcgtg gatcgccgcc
     5761 gcggccgcgc tgaaaattcc cgccgagggc ggcatggtca acgcctaccg ctggggcgac
     5821 ggcggcgcca ccgtgctgct gatgcatggc tgggaaggcg gcgtcgacga cttcgccgcg
     5881 ctggtgccgc agctggccgg cgcgggattt tc
//
LOCUS       DASZZC010000674         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1021850,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000674 DASZZC010000000
VERSION     DASZZC010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021850"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VHE37_14335"
     CDS             complement(<1..545)
                     /locus_tag="VHE37_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HVT36764.1"
                     /translation="MGTRKRKPLPAGEFTAQIEDLAADGRGVARVNGKVIFIADALPG
                     EMVTFRYLRVSRDIDEAQTVSVQRASPDRVTPACEHFGLCGGCALQHLSPPAQLAFKQ
                     KQLADAFERIGKVTPQQWAEPIAGPVWNYRRRARLGVKHVPKKGGMLVGFRERESSYL
                     AMLGSCRVLDARVGGKLAAIAA"
     gene            complement(545..1435)
                     /gene="cysM"
                     /locus_tag="VHE37_14340"
     CDS             complement(545..1435)
                     /gene="cysM"
                     /locus_tag="VHE37_14340"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HVT36765.1"
                     /translation="MNYSSLADFIGNTPLVRLTRMPGIGSNMLLAKLEGNNPAGSVKD
                     RPANSMIRRAEERGEIRPGDTLIEATSGNTGIALAMVAAARGYRMKLIMPEHMSAERR
                     AAMKAFGAEIILVTQQQGMEYARDLALEMQARGEGRVLDQFANPDNPRAHYEGTGPEI
                     WRDTRGSITHFVSSMGTTGTIMGCSRYFKEQQPSIQVVGVQPDGESSIPGIRKWPEAY
                     LPKIFERPRVDRIIEVSLQHAEDTTRLLAAKEGLFCGPSSGGAVWSALQLCRELSGAT
                     IACIICDRGDRYISTGVFPA"
     gene            1474..>3011
                     /locus_tag="VHE37_14345"
     CDS             1474..>3011
                     /locus_tag="VHE37_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT36766.1"
                     /translation="MPELKKIWSERFDGWYWLALPVLSFMGMMQVATWLGLQPDPTLG
                     VRALRTLIGFLGLWLGAIGAARIAAAQKKLLEVARHFSSGRLEARAPSHWHGLTGELA
                     EQLNTLGKMMTQYRDHQDHMVMQTTSRLRQDQERLSELNAELRRALRESQNAARAQSE
                     LFSNLSHELRTPLTAILGYADLLRRSSLSPEQGGHIETLDRSARGMLSMINDLLDWSR
                     IEAGRLKLNEESFDVLETVEDTGALLAPLAYDKHLELIRIFYHDVPRKLLGDPQRLRQ
                     IMTNLLSNAIKFTEAGEVALRVMRERDDEARVWLRFSVTDSGIGISPEALARLFKPFQ
                     QAGSGVGGSGLGLSITRKLAELMGGKVEVDSVPGGGSTFSVTLPFRLAEASVAQAVPD
                     TRLHERAAWLYEPHPIARLALMHWLEFWGLRVRTFESLDMLRSTLAGIATNLRPAVII
                     TGLSGEAAASTEAMSFYAFCTQQRLPLLVLLASSSLDLRERVLKAGAAGCLPKAAGHA
                     RLQS"
BASE COUNT          459 a         1047 c         1025 g          480 t
ORIGIN      
        1 gccgcaatcg ccgccagctt cccgccgacc cgcgcgtcga gcacgcggca gctgccgagc
       61 atggccaggt aggaactctc gcgctcgcgg aaaccgacca gcatgccgcc cttcttcggc
      121 acgtgcttca cgcccaggcg cgcgcggcgg cggtagttcc acaccgggcc ggctatcggt
      181 tcggcccatt gctgtggcgt caccttgcca atgcgctcga aggcatcggc cagctgtttc
      241 tgcttgaacg ccagctgtgc tggcggcgac aggtgctgca gcgcgcagcc gccgcacagg
      301 ccgaagtgct cgcacgccgg ggtgacgcgg tcgggcgacg cccgctgcac gctcacggtc
      361 tgcgcctcgt cgatatcgcg cgacacgcgc aggtagcgga aggtcaccat ctcgccgggc
      421 agcgcatcgg cgatgaagat caccttgccg ttgacgcgcg ccacgccgcg gccgtcggcg
      481 gcgaggtctt cgatctgcgc ggtgaactcg ccggcgggca gcggcttgcg cttgcgggtg
      541 cccatcaggc cgggaacact ccggtggaaa tgtagcggtc gccgcggtcg cagatgatgc
      601 aggcgatggt ggcgccggac agttcgcggc acagctgcag cgccgaccac accgcgccgc
      661 ccgacgacgg gccgcagaac aggccttcct tggccgccag caggcgcgtg gtgtcttcgg
      721 cgtgctgcag gctcacctcg atgatgcggt cgacgcgcgg ccgctcgaag attttcggca
      781 ggtaggcttc cggccatttg cggatgccgg ggatggacga ctcgccatcg ggctgcacac
      841 cgaccacctg gatcgacggc tgctgctcct tgaaatagcg cgagcagccc atgatggtgc
      901 cggtggtgcc catgctggag acgaaatgcg tgatgctgcc gcgcgtgtcg cgccagattt
      961 ccggcccggt gccttcgtag tgcgcacgcg ggttgtcggg attggcgaac tggtcgagca
     1021 cgcgcccttc gccgcgcgcc tgcatctcca gcgccaggtc gcgcgcgtat tccatgccct
     1081 gctgctgcgt gaccaggatg atctcggcgc cgaaagcctt catcgccgcg cgccgctcgg
     1141 ccgacatgtg ctcgggcatg atcagcttca tgcggtagcc gcgcgcggct gccaccatgg
     1201 caagcgcgat gccggtattg ccgctggtgg cctcgatcag cgtgtcgccg ggcctgatct
     1261 cgccgcgctc ctcggcgcgc cggatcattg aattggccgg gcggtccttg accgaacccg
     1321 cggggttgtt gccctccagc ttggccaaga gcatgttgct gccgatgccc ggcatgcggg
     1381 tcaggcgcac cagcggagta ttgccgataa aatcggccaa tgaggaatag ttcatcgcgg
     1441 cggcatttta aagcgtgaaa ctgcagcgca cctgtgcctg aactgaaaaa aatctggtcg
     1501 gagcgcttcg acggctggta ctggctggca ctgccggtgc tgtcattcat gggcatgatg
     1561 caggtggcca cctggctcgg cctgcagccc gaccccacgc tgggcgtgcg cgcgctgcgc
     1621 acgctgatcg gcttcctcgg cctgtggctc ggcgccatcg gcgccgcgcg catcgccgcc
     1681 gcgcagaaga aattgctgga agtggcgcga catttctcca gcgggcgcct ggaggcgcgc
     1741 gcgccgtcgc actggcacgg gctcaccggc gaactggccg agcagctcaa cacgctgggc
     1801 aagatgatga cgcagtaccg cgatcaccag gaccacatgg tgatgcagac cacctcgcgc
     1861 ctgcgccagg accaggagcg cctgtccgaa ctcaacgccg agctgcgccg cgcgctgcgc
     1921 gaatcgcaaa acgccgcgcg cgcgcagtcc gaattgttct ccaacctgtc gcacgagctg
     1981 cgcacgccgc tgaccgccat cctgggctac gccgacctgc tgcgccgctc gagcctgtcg
     2041 ccggaacagg gcggccacat cgagacgctc gaccgcagcg cgcgcggcat gctgtcgatg
     2101 atcaatgacc tgctggactg gagccgcatc gaggccggcc ggctcaagct caacgaggaa
     2161 agcttcgacg tgctggagac ggtggaggac accggcgcgc tgctggcgcc gctggcctac
     2221 gacaagcacc tcgagctgat ccgcatcttc taccacgacg tgccacgcaa gctgctgggc
     2281 gatccgcagc gcctgcgcca gatcatgacc aacctgctgt ccaacgccat caagttcacc
     2341 gaggccggcg aagtggcgct gcgcgtgatg cgcgaacgcg acgacgaggc gcgcgtgtgg
     2401 ctgcgcttct cggtgaccga cagcggcatc ggcatctcgc cggaagcgct ggcgcgcctg
     2461 ttcaagccgt tccagcaggc cggcagcggc gtcggcggca gcggcctggg cctgtcgatc
     2521 acgcgcaagc tggccgagct gatgggcggc aaggtggaag tggacagcgt gccgggcggc
     2581 ggctcgacct tctccgtcac cctgccgttc cgcctggccg aggccagcgt ggcgcaggcc
     2641 gtgcccgaca cacgcctgca cgagcgcgcc gcctggctgt acgagccgca cccgatcgcg
     2701 cgcctggcgc tgatgcactg gctggaattc tggggcctgc gcgtgcgcac cttcgaatcg
     2761 ctggacatgc tgcgttccac gctggccggc atcgccacca acctgcgccc ggcggtgatc
     2821 atcaccggcc tgtcgggaga ggcggccgcc agcaccgagg ccatgtcgtt ctacgcgttc
     2881 tgcacgcagc agcgcctgcc gctgctggtg ctgctggcct catcctcgct ggacctgcgc
     2941 gagcgtgtgc tcaaggccgg cgccgccggc tgcctgccca aggccgcggg ccatgcgcgc
     3001 ctgcagtcag a
//
LOCUS       DASZZC010000675         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1150234,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000675 DASZZC010000000
VERSION     DASZZC010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150234"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2368)
                     /locus_tag="VHE37_14350"
     CDS             complement(<1..2368)
                     /locus_tag="VHE37_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36767.1"
                     /translation="MHRFRLALMVAAASMWSVAALPQEAAPADPAPNANQPAESVPTI
                     PVAPAQPAEIAPAQSAGEATHLDDIVVTATKRATPVRRIAGTLTVLSGEDLERQGVQG
                     IDQIVSLVPGVNLADDGQGQAKRVTIRGISADTNVNFTAGTLFGDIPFSDPYIPKVQM
                     DPNPFDMATVEILKGPQGTLFGGSGLNGMIRYVPEAPAFDGTHLKYYAQFNDFPGNGG
                     RGWSSGAVLNAPFAGDSAALRLMGFHRDAPGYVDNTRTGAKDVNSLSQYGYRGMLTWL
                     PAAGWKVTLMGVGQHTLQDDVAFTDNFNGQLSRNNTPRPSPTETTYTLGNLGLEHSLG
                     WADFVSQTSGFTKKWQAFLDSSRIAADGSLPVLGVAGFDQSHAFAQELRLVSPADQDS
                     PWKWLGGAFYYRTHLFDCSEAFADAAGALGLPGVIDLGSLLPQPLVGVTAGVAATPCA
                     QNMAYARGTLDIAHLAGDIQLEERALFGELGRRFGEQWELTLGARLYRTQSSGTVSNE
                     GALYSAQTQGLPSTRNAGVKEQGVSPKASLVYMPTHDVRAYFTASRGFRFGGIQIGSS
                     TITTTIPPVFKSDSLWNYELGLRTDWLDRTLQLDSSLYLIDWKNPQVFQQSRDGLVTF
                     IDNVGGAQGRGVDFSARWLVPYLNGLTLTESASLNRTVTTVPFDSSTGKHVPSGSAWP
                     LAPRWQTSTTLAYALPLESWRSAIALRHTFSSSACNVIECGAQVLGYRTFNLSLSAAP
                     LAIAWMPEISLSLDNLTDERGISTVAQSTTLGNAVNYIPPRALTLRLGG"
BASE COUNT          384 a          828 c          865 g          400 t
ORIGIN      
        1 ttccgccgag gcgcagcgtg agcgcgcgcg gcggaatgta gttgaccgcg ttgcccagcg
       61 tggtcgactg cgccaccgtg ctgatgccgc gctcgtcggt gagattgtcc agcgacaggc
      121 tgatctccgg catccacgcg atggccagcg gtgccgccga caggctgagg ttgaacgtgc
      181 ggtagccgag cacctgcgcg ccgcattcga tcacgttgca ggcggaactg gaaaaggtgt
      241 ggcgcagcgc gatggccgag cgccagcttt ccagcggcag cgcgtaggcc agcgtggtcg
      301 aggtctgcca ccgcggcgcc agcggccacg ccgagccgct gggcacatgc ttgccggtgg
      361 aggaatcgaa cggcaccgtg gtcaccgtgc ggttcagcga ggcggattcg gtcagcgtca
      421 ggccgttgag gtacggcacc agccagcgcg cggaaaagtc cacgccacgg ccctgcgcgc
      481 cgccgacgtt gtcgatgaag gtgaccaggc cgtcgcgcga ctgctggaac acctgcggat
      541 tcttccagtc gatcaggtat agcgagctgt cgagctgcag cgtgcggtcg agccagtcgg
      601 tgcgcagccc cagctcgtaa ttccacaggc tgtcggactt gaacaccggc ggaatggtgg
      661 tggtgatggt ggagctgccg atctggatgc cgccgaagcg gaagccgcgc gaggcggtga
      721 agtacgcgcg cacatcgtgc gtgggcatgt acaccagcga ggctttcgga ctgacgccct
      781 gctccttcac gccggcgttg cgcgtgctcg gcagcccctg cgtctgcgcg ctgtacagtg
      841 cgccttcgtt gctcaccgtg ccggacgatt gcgtgcgata caggcgcgcg cccagcgtga
      901 gttcccactg ctcgccgaag cgccgcccca gttcgccgaa cagcgcgcgc tcttccagct
      961 ggatgtcgcc ggccaggtgc gcgatgtcga gcgtgccgcg cgcataggcc atgttctgcg
     1021 cgcacggggt cgccgccacg ccggcggtga cgcccaccag cggctgcggc agcagcgagc
     1081 cgaggtcgat cacgccgggc aggccgagcg cgcccgctgc atcggcgaac gcctcggaac
     1141 aatcgaacag gtgcgtgcgg tagtagaacg cgccgcccag ccatttccac ggcgagtctt
     1201 ggtcggccgg cgacaccagc cgcagttcct gcgcaaacgc atgcgactgg tcgaagccgg
     1261 caactccgag caccggcaac gagccgtcgg cggcgatgcg cgaggaatcg aggaacgcct
     1321 gccacttctt ggtgaagccc gacgtctgcg aaacaaagtc ggcccagccg agggaatgct
     1381 ccaggcccag gttgcccagc gtgtaggtgg tctcggtcgg gctcggccgc ggcgtgttgt
     1441 tgcgtgacag ctggccgttg aagttatcgg tgaaggccac gtcgtcctgc agcgtgtgct
     1501 gccccacgcc catcagcgtc accttccagc cggcggcggg cagccaggtc agcatgccgc
     1561 ggtagccgta ctgcgacagc gagttcacgt ccttggcgcc ggtgcgcgtg ttgtccacgt
     1621 aacccggggc atcgcggtgg aagcccatca ggcgcagcgc ggcgctgtcc ccggcgaacg
     1681 gcgcgttgag cactgcgcca gagctccagc cgcggccgcc attgccgggg aaatcgttga
     1741 actgcgcgta gtacttcaag tgcgtgccgt cgaaggctgg cgcctccggc acatagcgga
     1801 tcatgccgtt caggcccgag ccgccgaaca gcgtgccctg cgggcccttg aggatctcca
     1861 ccgtcgccat gtcgaacggg ttgggatcca tctgcacttt cggaatgtac ggatcggaaa
     1921 acgggatgtc cccgaacagc gtgccggcgg tgaagttcac gttggtgtcg gcggagatgc
     1981 cacggatggt gacgcgcttg gcctgccctt gcccgtcgtc ggcgaggttg acgccgggca
     2041 ccagcgagac gatctggtcg atgccctgca cgccctgccg ctcaaggtct tcgcccgaca
     2101 gcacggtgag cgtgccggcg atcctgcgca ccggcgtggc gcgcttggtc gcggtgacca
     2161 cgatgtcgtc gaggtgcgtg gcttcgcccg cactctgcgc gggcgcaatc tcggcgggct
     2221 gcgccggcgc caccgggatg gtgggcaccg actcggctgg ctgatttgcg ttcggcgctg
     2281 ggtcggccgg ggccgcctcc tgcggcagcg cggccacgct ccacatcgac gctgccgcga
     2341 ccatcaacgc cagcctgaat ctatgcatct gccacatcct ccatcggccc gcgcacctgg
     2401 cgcagacgtt ccacgtgatg cgcaccgacc tccgccaccg atttgcagcc gagcaggcgc
     2461 aggctgcgat cgagttc
//
LOCUS       DASZZC010000676         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_961004,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000676 DASZZC010000000
VERSION     DASZZC010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961004"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /gene="gspF"
                     /locus_tag="VHE37_14355"
     CDS             <1..1236
                     /gene="gspF"
                     /locus_tag="VHE37_14355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HVT36768.1"
                     /translation="SKSAVAAFEYAALDASGKQQKGLIEGDTPRHARQLLRDRGLAPL
                     AITEVAEAKAAKGTVGRMFGGGSGISTTELSLFTRQLAILVRSGLPLDEALTAVAEQS
                     ESRKVKRITLGVRAGVVEGQTLADSLNQFPNAFPPLFRATVEAGEQSGKLDNILERLA
                     DYVERRQAMQGTIFKAMIYPVFLSLVAIGVVTLLLAYVVPQVVQVFKDINAELPPLTR
                     GLIAFSNFLRDWGVYLAVLLAIGIGVFLQMMRAEAFRRRVHLSVLRLPIVGRLTRGAN
                     TGRFTRTLGILFGSGVPILEAMRIGTQVVLNLPMREAIEEATIKVREGASLSKSLNAS
                     KLFPPIAVHMIASGEASGRLDEMLDRAAENQEREVETLVGALMSLAEPVMILVMGGVV
                     MVIVLAILLPIFDLNQLVK"
     gene            complement(1267..>1606)
                     /locus_tag="VHE37_14360"
     CDS             complement(1267..>1606)
                     /locus_tag="VHE37_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36769.1"
                     /translation="SIAFFTTAQEMQIASQWARSKQSLGNPNKDRSAFAGKFETLVAR
                     TGSGITTKGSNKVLQGVVQAMEAAGMDLREWAIPHNLWESMEVKKQDKDEDEPAEGTD
                     GADQPQEPKL"
BASE COUNT          246 a          537 c          537 g          286 t
ORIGIN      
        1 agtaaatccg ccgtggcggc cttcgaatac gccgcgctcg acgcgtccgg gaaacaacag
       61 aagggcctga tcgaaggcga cacgccgcgc cacgcgcgcc agctgctgcg cgaccgcggg
      121 ctggcgccgc tggccatcac cgaggtggcc gaagcaaaag ccgccaaggg caccgtcggc
      181 cgcatgttcg gcggcggcag cggcatctcc accaccgagc tttccctgtt cacgcgccag
      241 ctggcgatcc tggtgcgctc aggcctgccg ctggacgagg cgctgaccgc ggtggccgag
      301 cagagcgaga gccgcaaggt caagcgcatc accctgggcg tgcgcgccgg cgtggtcgag
      361 gggcagacac tggccgattc gctgaaccag tttccgaacg cattcccacc gctgttccgt
      421 gccacggtcg aggccggcga gcagtcgggc aagctcgaca acatcctcga gcggctggcc
      481 gattacgtcg agcgccggca ggcgatgcag ggcaccatct tcaaggcgat gatctacccg
      541 gtgttcctgt cgctggtggc gatcggcgtg gtcacgctgc tgctggccta cgtggtgccg
      601 caagtggtgc aggtgttcaa ggacatcaat gccgaactgc cgccgctgac gcgcgggctg
      661 atcgccttct ccaatttcct gcgcgactgg ggcgtgtatc tcgccgtgct gctggccatc
      721 ggcatcggcg tgttcctgca gatgatgcgc gccgaagcgt tccgccgtcg cgtgcatctg
      781 agcgtgctgc gcctgcccat cgtcggccgg ctgacacgcg gcgccaacac cggccgcttc
      841 acccgcacgc tgggcattct gttcggctcc ggcgtgccga tcctcgaagc catgcgcatc
      901 ggcacgcagg tggtgctgaa cctgccgatg cgcgaggcca tcgaggaagc gaccatcaag
      961 gtgcgcgagg gcgcctcgct gtccaagtcg ctcaacgcca gcaagctgtt tccgccgatc
     1021 gccgtgcaca tgatcgccag cggcgaagcc tccggccggc tcgacgagat gctcgaccgt
     1081 gccgcggaaa accaggagcg cgaggtggaa acgctggtcg gtgcgctgat gagcctggcc
     1141 gaaccggtga tgatcctggt gatgggcggc gtggtgatgg tgatcgtgct ggcgattttg
     1201 ctgccgatct tcgatctcaa ccagctggtg aagtaacgcc ggcgctgccg gcgcgcgacg
     1261 gcgcgcttac agcttcggct cctgcggctg gtccgcgccg tccgtgcctt ccgccggttc
     1321 gtcctcgtcc ttgtcctgct tcttcacttc catgctttcc cacagattgt gcggaatggc
     1381 ccactcgcgc aggtccatgc cggcggcttc catggcctgc accacgccct gcagcacctt
     1441 gttagaaccc ttggtggtga tccccgagcc ggtgcgcgcg accagcgtct cgaacttgcc
     1501 ggcgaacgcg gaacgatcct tgttggggtt gcccaggctc tgcttggagc gggcccactg
     1561 cgaggcaatc tgcatttcct gcgcggtggt gaagaaggcg atggac
//
LOCUS       DASZZC010000677         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_249199,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000677 DASZZC010000000
VERSION     DASZZC010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249199"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VHE37_14365"
     CDS             complement(<1..449)
                     /locus_tag="VHE37_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HVT36770.1"
                     /translation="MSNSVSSRWPYFPLFLKLQGQRVLLVGAGTVAARKLELLLRAGA
                     SVEIVAPDVSADVRALREHSGMRVVAAQFAPGQLRGCKLAIAATDDAQLNRAVAQAAT
                     HANIPVNVVDDVELSTCIMPAVVDRAPLLAAISTGGAAPVLARRVRA"
     gene            complement(579..1373)
                     /locus_tag="VHE37_14370"
     CDS             complement(579..1373)
                     /locus_tag="VHE37_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT36771.1"
                     /translation="MEGLGFSAAGLLVGTAVGATGVGGGSLMTPILILFYGINPSIAV
                     GTDLIYASLSKSFGVVLHSRNKTVDWRIVGWLSAGSVPATLLTLMFLHMFGTGPGLDR
                     LIKLTLSLAIIVTASFAIAQDRVLGYVRAHEKLQVLRGLRQRWQIPATILCGALVGTV
                     VTISSVGAGVIGMMLLLLLYPGVAPIKLVGSDLAHAVLITGIAGLGHARLGTVDWHML
                     LFLLIGALPGIWLGTRVGFALSPIPLKRAIAVLLIFIGVTTLAKAF"
     gene            1485..>2084
                     /locus_tag="VHE37_14375"
     CDS             1485..>2084
                     /locus_tag="VHE37_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVT36772.1"
                     /translation="MKLRQLHYIHEVARRGLNVTAAAEALHTSQPGVSKQIRLLEDEL
                     GVEIFLRNGKHLAEITPAGKKVLEYAGRLLEQAGNIQNIAEEFRDSGHGDLSISTTHT
                     QARYALPPVIEKFRKRYPRVTLHLHQGSPPQIAQMAAEGQADFAIATEAMHHFEELVM
                     LPCYHWNRCVLVKPGHALAGKKKLTLKDIAAFPIVTYTFG"
BASE COUNT          441 a          678 c          655 g          310 t
ORIGIN      
        1 tgcgcgcgca cgcgccgtgc aagtaccggc gcggcgccgc cggtgctgat ggccgcgagc
       61 agcggcgcgc gatcaaccac cgccggcatg atgcacgtcg acagttccac gtcatcgacc
      121 acgttgaccg gaatgttcgc atgcgtggcc gcctgcgcca ccgcgcgatt gagttgagca
      181 tcgtccgtcg cggcgatggc aagcttgcaa ccgcgcagtt gcccgggcgc aaattgcgcc
      241 gcgaccaccc gcatgcccga gtgctcgcgc agcgcccgga cgtccgcact gacgtccggc
      301 gcgacgattt ccacgctcgc gccggcacgc agcagcaatt cgagcttgcg cgccgccacg
      361 gtgccggcgc cgaccagcag cacgcgctgc ccctgcagtt tcaggaacag cgggaagtaa
      421 ggccagcgcg aagacactga attgctcatg ccggtgagac tgccatttcg gcgccaaaaa
      481 cgctccaccg catgggactt ttatatgtta tatattattg tattgaatta tattcataca
      541 ataattaata acacaaaaaa tgcaggtgcg gtgacggctc agaatgcctt ggccagcgtg
      601 gtgacgccga tgaagatgag cagcacggcg atcgcgcgct tgaggggtat aggcgagagt
      661 gcaaaaccta cgcgcgtgcc gagccagatg ccgggcagcg caccgatcag caggaacagc
      721 agcatgtgcc agtccaccgt gcccagccgc gcgtgcccca gcccggcgat gccggtgatg
      781 agcaccgcgt gcgccaggtc gctgcccacc agcttgatcg gcgccacacc cgggtacagc
      841 agcaacagca gcatcatgcc gatcacgccg gcacccactg acgagatggt gaccacggtg
      901 ccgaccagcg cgccgcacag gatggtggcg gggatctgcc aacgctggcg cagaccgcgc
      961 agcacctgca acttctcgtg cgcgcgcaca tagccgagca cgcggtcctg cgcgatggcg
     1021 aagctggcgg tgacgatgat ggccagcgac agggtgagct tgatcagccg gtcgaggccg
     1081 gggccggtgc cgaacatgtg gagaaacatc agcgtgagca gcgtggccgg cacgctgccg
     1141 gcggagagcc agccgacgat tctccagtcc acggtcttgt tgcggctgtg caggaccacg
     1201 ccgaatgatt tggaaagact ggcgtagatc aggtcggtgc caaccgcgat cgatggattg
     1261 atgccgtaga acaggatcag gatcggcgtc atcagcgagc cgccgcccac accggtcgcg
     1321 cccactgcag tacccaccag caagccggct gccgaaaagc ccaagccttc catatgcgta
     1381 gttcccccaa aatagcggcg caagctagca gagccatgcc gattgctgaa ggaataattt
     1441 gcctatactt gaagccgaaa atggcataag ggaaggaccg ggccgtgaag ctcaggcagc
     1501 tgcactacat ccacgaagtg gccaggcgcg ggctcaacgt caccgctgcg gcggaggcgc
     1561 tgcatacctc gcaacctggc gtatcgaagc agatccgcct gctggaagac gagctgggcg
     1621 tggagatctt cctgcgcaac ggcaagcacc tggcggagat caccccggcc gggaaaaagg
     1681 tgctggagta cgcggggcgc ctgctggaac aggcgggcaa catccagaac atcgccgagg
     1741 aattccgcga cagcggccac ggcgacctgt cgatctccac cacgcacacg caggcgcgct
     1801 acgcgctgcc gccggtgatc gaaaaattcc gcaagcgcta cccgcgcgtc acgctgcacc
     1861 tgcaccaggg ctcgcccccg cagatcgcgc agatggcggc agaaggccag gccgacttcg
     1921 ccatcgccac cgaggccatg caccacttcg aggaactggt gatgttgccg tgctaccact
     1981 ggaaccgctg cgtgctggtc aagccgggcc acgcccttgc cggcaagaag aagctgacgc
     2041 tgaaggacat tgcagcattt cccatcgtca cctacacctt cggc
//
LOCUS       DASZZC010000678         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_113185,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000678 DASZZC010000000
VERSION     DASZZC010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113185"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHE37_14380"
     CDS             <1..327
                     /locus_tag="VHE37_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36773.1"
                     /translation="NQQALLDKIDCSLLAIAGENDKIVTVEAARKVMDIVGSADKQFE
                     TAPGGHAGVFAGSRAPQTTWTLAAEWLAPRSDYAAATSRGAARAPARKRASSRTKRRG
                     ASKGTT"
     gene            complement(228..1157)
                     /locus_tag="VHE37_14385"
     CDS             complement(228..1157)
                     /locus_tag="VHE37_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36774.1"
                     /translation="MPKMTMRDGARLHYLDIGRGPVCVLLHGFAMQSAMWLPFVAPLA
                     HRWRFIIPDLRGFGFSHRLPLAHQSILDQHAHDLHDLMEGLQLGRVHLAGLSMGACTS
                     LQYHRLYGFDRVHAYLNIDQSPCVRNDAQWRHGVLGERQEEMLARWAQLAQDLMACGR
                     DTPFAQLPGHLRRGLFSTLSEFLVFGFHHPAWQLLRHAAKQEPLMRYVAPTANWPIYL
                     DTLTSYQHDDYDWREDLRRVRIPMTVMIGMRSTMYPAQGQLAFRELVPHARIVRVENV
                     GHVVPFEAPLRFVRELARFLAGARAAPRLVAAA"
     gene            complement(1176..>1858)
                     /locus_tag="VHE37_14390"
     CDS             complement(1176..>1858)
                     /locus_tag="VHE37_14390"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT36775.1"
                     /translation="WPHLVKGMVRKGLLKELGPDFDIRTHFTPRYNPWDQRMCLVPDA
                     DLFQVLREGRADIVTDHIETFTPAGIRLKSGKELAADIIVTATGLNLQALGGAQLAVD
                     GQPVNLAQTMSYKGMMLSGVPNMALALGYTNASWTLKVDLTCDYVCRLLNHMRARGYR
                     QCTPRRDASVAEVPFIDFSSGYILRSIDKFPRQGSVAPWKLHQNYAMDILALRYGKVD
                     EPSLQYAE"
BASE COUNT          316 a          610 c          647 g          285 t
ORIGIN      
        1 aaccagcagg cgctgctcga caagatcgac tgctcgctgc tggccatcgc cggcgagaac
       61 gacaagatcg tcacggtcga ggcggcgcgc aaggtgatgg acatcgtcgg cagcgccgac
      121 aagcagttcg agaccgcgcc cggcggccat gccggcgtgt tcgctggcag ccgtgcgccg
      181 cagaccacct ggacgctcgc cgccgaatgg ctggcgccgc gctcggacta cgccgccgct
      241 accagccgcg gcgctgcgcg cgcgccggcc aggaagcgcg ccagctcgcg cacgaagcgc
      301 aggggcgcct cgaaaggcac cacgtgaccc acgttctcca cgcgcacgat gcgcgcgtgg
      361 ggcaccagtt cgcggaacgc cagctgccct tgcgccgggt acatggtgga gcgcatgccg
      421 atcatcaccg tcatcgggat gcgcacgcgg cgcaggtctt cgcgccagtc gtagtcgtca
      481 tgctggtacg aggtgagcgt gtcgaggtag atcggccagt tggcggtcgg cgcgacgtaa
      541 cgcatcagcg gctcctgctt cgccgcatgg cgcagcagct gccaggccgg gtggtgaaag
      601 ccgaacacca ggaattcaga cagcgtggaa aacaggccgc gccgcaggtg ccccggcaac
      661 tgtgcgaacg gcgtatcgcg gccgcaggcc atcaggtcct gcgccagctg ggcccacctg
      721 gccagcattt cctcctgccg ctcgccgagc acgccgtggc gccattgcgc gtcgttgcgc
      781 acacagggcg actggtcgat gttgaggtag gcgtgcacgc ggtcgaagcc gtacagccgg
      841 tggtactgca gcgaggtgca ggcgcccatc gacaggccgg cgagatgcac gcggcccagc
      901 tgcagtcctt ccatcaggtc gtgcaggtca tgcgcgtgct ggtccaggat ggactggtgc
      961 gccagcggca gccggtggct gaagccgaag ccgcgcaggt cgggaatgat gaaacgccag
     1021 cggtgcgcca gcggcgccac gaacggcagc cacatggccg attgcatggc aaagccgtgc
     1081 agcagcacgc acaccggccc gcggccgata tcgaggtaat gcaggcgcgc gccatcgcgc
     1141 atggtcatct tcggcatggg gtgtctcccg tggtgttatt ccgcgtattg cagcgaaggc
     1201 tcatccacct tgccgtagcg cagcgccagg atgtccatgg cgtagttctg gtgcagcttc
     1261 cacggcgcca cgctgccctg gcgcggaaac ttgtcgatgg agcgcaggat gtagcccgag
     1321 gagaaatcga tgaacggcac ttccgcaaca gaagcatcgc gccgcggcgt gcattgccgg
     1381 taaccgcgcg cgcgcatgtg gttgagcagg cggcagacgt aatcgcaggt caggtcgact
     1441 ttcaaagtcc acgacgcatt ggtgtagccc agcgccagcg ccatgttcgg cacgcccgac
     1501 agcatcatgc ccttgtagct catggtctgc gccaggttga ctggctgccc atcaaccgcc
     1561 agctgcgcgc cgccgagcgc ctgcaggttg aggccggtgg cggtgacgat gatgtcggcg
     1621 gccagctcct tgccggactt caggcggatg ccggccggcg tgaacgtctc gatgtgatcg
     1681 gtgacgatgt cggcgcggcc ctcgcgcagc acctggaaca ggtcggcgtc gggcaccagg
     1741 cacatgcgct ggtcccacgg gttgtagcgc ggcgtgaagt gcgtgcggat gtcgaagtcc
     1801 ggcccgagct ccttgagcag gcccttgcgc accatgcctt tcaccaggtg cggccagc
//
LOCUS       DASZZC010000679         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1022045,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000679 DASZZC010000000
VERSION     DASZZC010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022045"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VHE37_14395"
     CDS             complement(<1..287)
                     /locus_tag="VHE37_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36776.1"
                     /translation="MQLQNKVAIVTGGARGIGAGIARCLADAGAQVAVVDLDGDAAEA
                     TAAALPVRGIGLRADVSQENEMEQAAQQIVQRLGALDFLINNAGGGKPGSG"
     gene            complement(290..1459)
                     /locus_tag="VHE37_14400"
     CDS             complement(290..1459)
                     /locus_tag="VHE37_14400"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT36777.1"
                     /translation="MNDLSAGAPAVHGTVARGWERVREAFAQNFARNDAFRECGAALA
                     VYHRGEAVVDLWGGHADRARTRPWQRDTLINVYSTSKGIVATAVAMLVDEGRLDYDAR
                     VTRWWPEFGAAGKESTTVAQLLSHQAALTGFTDPLPLPVEKLYDWDSCVRALAAQAPC
                     WPPGTRTSYHAMTWGYLAGELVRRASGMSVGRYVRERIARRLGADVHIGLGKGDEPRV
                     AEMLAPKTAPDLAALTQPRESLLALVSPQLDPEICNRREWRAAEIPAASGQASAQGVA
                     RVYAALASGGEFAGARLLSRDTLARATARVRGQTDLLLGFTDAWSMGWAWNQHGMLGP
                     RAGTFGHSGWGGSFGCADADAGIAIGYVCNQMGAQLVGDPRSVSLCAAIFDCAGN"
     gene            complement(1456..2709)
                     /locus_tag="VHE37_14405"
     CDS             complement(1456..2709)
                     /locus_tag="VHE37_14405"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVT36778.1"
                     /translation="MQAKTRKMKQDAQRAQPPPALLDAAAVEFNARGIAGARLAQIGA
                     AAGIKRSALYYYFDSREDLAFRCYLRECRQVADDLAAARTADDGLARLHAYLRRALDA
                     ARPPAVVLSEIPYLGHERRRRVEAELARNMRTLTGFVEEGIADGSIRACNARVAAQAI
                     YGMVVWVPLAGEWVVDAGADVRRHAAAALWDLVQRGTAAHAGADVRCTLEVNDFAFRH
                     GNAFDRREASALKVELLVQTASRLFNRHGIDGTSLDDITAELGATKGAFYHHITEKKA
                     LVLRCAQRAFDLYDRFADAAETHGRNGLERSMIGLHLNVQAQASALSPLSPLTGLEVL
                     PARSLKDLRARSLRVEQRFEAFNHGGIRDGSLRRFDVRTLSLAGAGAFGWISKWYDEA
                     QGPSPRELADEMVNLFVHGLRSSRR"
     gene            2845..>3750
                     /locus_tag="VHE37_14410"
     CDS             2845..>3750
                     /locus_tag="VHE37_14410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT36779.1"
                     /translation="MKRWTGGLLLVLALGAAAQEPAADTAPAAADDSSAAPAADAAPS
                     ASAQPSAAEVPTIAVPQKAEVPAPPQAAQLDEVVVTAQKRAQRLVDVPINVSAMTRDD
                     VQKTRIEQVRDVAGYMPNVDIKEQVPGAIPVVSIRGVGLDDFSSTNSPAAGIYLDQVT
                     LSSLALMSFDLYDIERIEVLKGPQGTLYGRNSTAGAINVLSASPVFEREAYLKAGAGT
                     YKTNDVEGMFNLPLAETFAVRVAGKLIRQGRGFWDSRLDANDAANYTIPPTGVLPALV
                     GVPPLRARDTSGDPVVRDIGKRDILL"
BASE COUNT          554 a         1355 c         1299 g          542 t
ORIGIN      
        1 atgccgctgc ccggcttgcc accgccggcg ttgttgatca ggaaatcgag cgcgcccaac
       61 cgttgcacga tctgctgcgc cgcctgctcc atctcgttct cctgcgacac gtcggcgcgc
      121 aagccgatgc cgcgcaccgg cagcgcggcg gcagtggcct cggccgcgtc accatcgaga
      181 tcgaccacgg ccacctgcgc gcccgcatcc gccaggcagc gcgcgatgcc ggcgccgatg
      241 ccgcgcgcgc cgccagtgac gatcgcaacc ttgttctgta actgcatgat cagttccccg
      301 cgcagtcgaa gatggcggcg cacagcgaca ccgagcgcgg atctcccacc agctgcgcgc
      361 ccatctggtt gcacacgtag ccgatggcga tgccggcgtc cgcatcggcg cagccgaacg
      421 agccgcccca gccgctgtgg ccgaaggtgc cggcgcgcgg gccgagcatg ccgtgctggt
      481 tccacgccca gcccatgctc caggcgtcgg tgaagccgag cagcaggtcg gtctgtcctc
      541 gcacccgcgc ggtggcgcgt gccagcgtgt cgcgcgacag cagccgcgca ccggcgaact
      601 cgccgccgct ggcgagcgct gcgtacacgc gcgcgacgcc ctgcgcgctg gcctggccgc
      661 tggcggcggg gatttccgcc gcgcgccact cgcgccggtt gcagatttcc gggtcgagtt
      721 gcgggctgac cagcgccagc agcgattcgc gcggctgcgt cagcgcggcc aggtccggcg
      781 ccgtcttcgg cgccagcatt tccgcaacac gaggctcgtc gcccttgccg agaccgatgt
      841 gcacgtcggc gcccagccgt cgcgcgatgc gctcgcgcac gtagcggccc acgctcatgc
      901 cgctggcgcg ccgcaccagt tcgccggcga gatagcccca ggtcatggcg tggtagctgg
      961 tgcgtgtgcc cggcggccag cacggcgcct gcgcggcgag cgcgcgcacg caggaatccc
     1021 agtcgtaaag tttttccacc ggcagcggca gcggatcggt gaagccggtg agcgccgcct
     1081 ggtgcgacag cagctgggcg acggtcgtcg actccttgcc ggccgcgccg aactccggcc
     1141 accagcgcgt cacgcgcgcg tcgtagtcga gccggccttc atcgaccagc atggcgacgg
     1201 cggtggcgac gatgcctttg ctggtggagt acacgttgat cagcgtgtcg cgctgccacg
     1261 gccgcgtgcg cgcgcggtcg gcgtggccgc cccacaggtc gaccacggcc tcgccgcggt
     1321 ggtacaccgc cagcgccgcg ccgcattcgc ggaacgcgtc attgcgtgca aagttctgcg
     1381 cgaaggcctc gcgcacgcgc tcccagccgc gcgccaccgt gccgtgcacg gcgggcgcac
     1441 ccgcggacaa gtcgttcatc gtctgctgct cctcagtcca tggacaaaca ggttcaccat
     1501 ctcgtccgcc agttcgcgcg gcgatggccc ctgcgcctcg tcgtaccact tggaaatcca
     1561 gccgaacgcg ccggcgccgg ccagcgacag cgtgcgcacg tcgaagcggc gcaggctgcc
     1621 gtcgcggatg ccgccatggt tgaacgcctc gaagcgctgc tccacgcgca gcgagcgcgc
     1681 gcgcaggtcc ttcaggctgc gcgccggcag cacttccaga ccggtcagcg gcgacagcgg
     1741 cgacagcgcg ctggcctgcg cctgcacgtt caggtgcagg ccgatcatcg aacgctccag
     1801 tccgttgcgg ccatgcgttt ccgcggcgtc ggcgaagcgg tcgtagaggt cgaacgcgcg
     1861 ctgggcgcag cgcagcacca gcgccttctt ctcggtgatg tggtgataga acgcgccctt
     1921 ggtggcgccg agctcggcgg tgatgtcgtc cagcgaagtg ccgtcgatgc cgtggcggtt
     1981 gaacaggcgc gacgccgtct gcaccagcag ttcgaccttc agcgccgacg cttcgcgacg
     2041 gtcgaaggcg ttgccgtggc ggaaggcgaa gtcgttcacc tcgagcgtgc agcgcacatc
     2101 ggcgccggca tgggcagcgg tgccgcgctg caccaggtcc cacagcgcgg cggcagcgtg
     2161 gcggcgcacg tccgcgccgg catcgaccac ccactcgccg gccagcggca cccacaccac
     2221 catgccgtag atggcctgcg cggcgacgcg cgcgttgcag gcgcggatcg agccgtcggc
     2281 gatgccctct tcgacaaacc cggtgagcgt gcgcatgttg cgcgccagct cggcttccac
     2341 ccggcggcgg cgctcgtggc ccaggtacgg aatttcgctc agcaccaccg ccggtggccg
     2401 cgcggcgtcg agcgcgcggc gcaggtaggc gtgcaggcgc gcgagcccgt cgtccgccgt
     2461 gcgcgcggcg gcgagatcgt cggccacctg ccggcattcg cgcaggtagc agcggaaagc
     2521 gaggtcttcg cgcgaatcga agtagtagta cagcgccgag cgcttgatgc cggccgcggc
     2581 gccgatctgc gccagccgcg ctccggcgat gccgcgcgcg ttgaattcca ccgccgccgc
     2641 atcgagcagc gccggcggcg gctgggcgcg ctgcgcgtcc tgcttcatct tgcgggtttt
     2701 ggcctgcacg cgggttccgg tggtgggtcg ctgtcagctt gacagatttg ctgacgtaat
     2761 gttatcacta cataccgaag tgtcaaatag aagatagaac cagcgcttcg gcaagggagc
     2821 gcggcaggcg ggctggagag agcgatgaaa agatggacag gtgggctgct gctggtgctg
     2881 gcactgggcg ccgcggcgca ggaaccggcg gcggatacag cgccggcggc cgcagacgac
     2941 agcagcgccg cgccggcagc agatgccgcg ccgtccgcgt ccgcgcagcc atcggctgcc
     3001 gaggtgccga ccatcgccgt cccgcagaag gccgaggtgc cggcgccgcc gcaggccgcg
     3061 cagctcgatg aagtagtggt caccgcgcag aaacgcgcgc agcgcctggt cgacgtgccg
     3121 atcaatgtct cggccatgac ccgagacgac gtgcagaaga cgcgcatcga gcaggtgcgc
     3181 gacgtggccg gctacatgcc caatgtcgac atcaaggaac aggtgccggg cgccatcccg
     3241 gtggtgtcga tccgcggcgt cggcctcgat gacttctcct ccaccaacag cccggccgcc
     3301 ggcatctacc tggaccaggt cacgctgtcg tcgctggcgc tgatgagctt cgatctttac
     3361 gacatcgagc gcatcgaggt gctgaagggc ccgcagggca cgctctacgg ccgcaactcc
     3421 accgccggcg ccatcaacgt gctgtccgcc tccccggtgt tcgagcgcga ggcttacctg
     3481 aaggccggcg ccggcaccta caagaccaat gatgtcgaag gcatgttcaa cctgccgctc
     3541 gccgaaacct tcgccgtgcg cgtggccggc aagctgatcc gccagggccg cggtttctgg
     3601 gacagccggc tcgacgccaa cgatgccgcc aactacacca tcccgcccac cggcgtgctg
     3661 ccggcgctgg tcggcgtgcc gccgctgcgt gcgcgcgaca cctccggcga cccggtggtg
     3721 cgcgacatcg gcaagcgcga catcctgctc
//
LOCUS       DASZZC010000680         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_249394,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000680 DASZZC010000000
VERSION     DASZZC010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249394"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..296
                     /locus_tag="VHE37_14415"
     CDS             96..296
                     /locus_tag="VHE37_14415"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HVT36780.1"
                     /translation="MNDQRLIELEERFAYQEATLRDLNDVITRQQQQIDRLEQICRML
                     VERVARQDISKGTPEEEIPPHY"
     gene            complement(290..1201)
                     /locus_tag="VHE37_14420"
     CDS             complement(290..1201)
                     /locus_tag="VHE37_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT36781.1"
                     /translation="MRPATLRGASTVFEGIAIATIEFAAADGARSAAAVFAATRNDAP
                     VVVIWPAMGVPARFYEPFAPVLGAAAGAHVVVPDLRGIGASSLRASRQVNFGYRELVE
                     LDCPAAVAAVRAHFPHSPIYLLGHSLGGQVSALYAAREPQAVKGLIFVASGNVWYRGW
                     RFPQNASFLLFTQAAGFIAQTLGYFPGRKLGFGGTEARWLMRDWARTARDGRYRLGGS
                     SFDYEAALAHLQLPLLGIGFAHDHFAPHRAAENLLHKMKAARLTHLKLTAAETEGRKL
                     DHFSWVKQPQPVAQRIAHWLAQQGAVQ"
     gene            complement(1228..>2495)
                     /locus_tag="VHE37_14425"
     CDS             complement(1228..>2495)
                     /locus_tag="VHE37_14425"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVT36782.1"
                     /translation="MFSLFAGVIMSCYLLFWLNLRERTQAAAALAHQRIALAVAAAQL
                     GLWESDAASGEMIVDDIAAHVHGIPGGAGTTTFKRTLQQLTPADAERVKAAAHHALAT
                     RSSYQIEYQVLWPDNTIHTVSARGQGEYDDDGRAVRMIGVAWDSTAHRREHQELRHLR
                     DQLEMAQQITGAGTWCFDLETRECTLDPNACLIFGLPVKDRRRAYEALLAMVHPDDRE
                     RVSQAVEDAIFRQRPYAVEVRIVTASGRIRVVRSHAKVQYADAGGPALRMVGACWDVT
                     QTRRGEAQTQAAQERMLRQDAALQIGSWSYFPQENRIQADAALVALYDLGHGDDGITL
                     DQVLQRVHAEDRQRVIAELAQLLQAGGEQRTDYRAVWADGSVRQLHVRGIALRDGNGA
                     TTQIVGATIDVTRLYRTLDRTLHGPEAPQ"
BASE COUNT          399 a          824 c          874 g          398 t
ORIGIN      
        1 gtcgtcgacc acgtcgttga tgaaactttt ccagaatccg ttctgcgggg acatgtttcc
       61 cgatagacca ccgcgccggt taaagtgcgc aggccatgaa tgaccagcgc ctgatcgaac
      121 tcgaagaacg cttcgcctac caggaagcga cgctgcgcga tctaaacgac gtgatcacgc
      181 gccagcagca gcagatcgac cgcctcgagc agatctgccg gatgttggtg gaacgggtgg
      241 cgcggcagga catttccaag ggcacgcccg aggaagagat tccgccgcat tactgaacgg
      301 cgccttgctg cgccagccag tgcgcgatgc gctgcgcgac cggttgcggc tgcttcaccc
      361 agctgaagtg atcgagcttg cgtccttccg tttccgcggc ggtgagcttg aggtgcgtga
      421 gccgcgcggc tttcatcttg tgcagcaggt tctccgccgc gcgatgcggc gcgaaatggt
      481 catgcgcgaa accgatgccc agcaatggca gttgcaagtg ggccagcgcg gcttcgtaat
      541 cgaacgacga gccgccgagc cggtaacgcc cgtcgcgcgc ggtgcgcgcc cagtcgcgca
      601 tcagccagcg cgcctcggtg ccgccgaaac ccagcttgcg gcccggaaaa tagccgagcg
      661 tctgcgcgat gaacccggcc gcctgcgtga acaacaggaa cgaggcattc tgcggaaagc
      721 gccagccgcg gtaccacacg ttgccgctgg cgacgaagat caatcccttg acggcctgcg
      781 gttcgcgcgc ggcgtacagc gcactgacct ggccgcccag gctgtggccc agcaagtaga
      841 tgggcgaatg cggaaagtgc gcacgtactg cggcgacggc cgcggggcaa tcgagttcca
      901 ccagttcgcg gtagccgaaa ttcacctggc gcgaggcacg cagcgaactg gcgccgatgc
      961 cgcgcaggtc gggaaccacg acgtgcgcgc cggcggcggc gcccagcaca ggcgcgaaag
     1021 gttcgtagaa gcgcgccggc acacccatcg ccggccagat gaccaccacc ggcgcatcgt
     1081 tgcgggtggc ggcaaaaacc gcggcggcgc tgcgcgcgcc gtcggcggcg gcaaattcga
     1141 tggtggcgat ggcgattccc tcgaagacag tcgatgcgcc gcggagtgta gccggccgca
     1201 ccggcgggtc cgcaatgacc tgcggggtta ttgcggcgct tcggggccgt gcagcgtgcg
     1261 gtccagcgtg cggtagaggc gggtgacgtc gatggtggcg ccgacgatct gcgtggtggc
     1321 gccgttgccg tcgcgcagcg cgatgccgcg cacatgcagc tggcgcaccg agccgtcggc
     1381 ccagacggca cggtaatcgg tgcgctgctc gccgccggcc tgcagcagct gcgccagttc
     1441 ggctatcacg cgctggcggt cttcggcatg gacgcgctgc agcacctggt cgagcgtgat
     1501 gccgtcgtcg ccatggccca ggtcataaag cgccaccagc gcggcgtcgg cctggatgcg
     1561 gttctcctgc gggaaatagc tccacgagcc gatctgcagc gctgcatcct ggcgcagcat
     1621 gcgttcctgc gcagcctgcg tctgcgcttc gccgcggcgg gtctgcgtca cgtcccagca
     1681 ggcaccgacc atgcgcagcg ccggtccgcc ggcgtcggcg tattgcacct tggcatgcga
     1741 gcgcaccacg cggatgcggc ctgaggcggt gacgatgcgc acctccactg cgtacggccg
     1801 ctgccggaag atggcgtcct cgaccgcctg gctgacgcgc tcgcggtcgt ccgggtggac
     1861 catcgccagc agcgcttcat aggcgcggcg gcggtccttc accggcaggc cgaatatcag
     1921 gcaggcgttc gggtccagcg tgcactcgcg cgtctccagg tcgaaacacc aggtgccggc
     1981 gccggtgatc tgctgcgcca tttccagctg gtcgcgcagg tggcgcagtt cctggtgctc
     2041 gcggcggtgc gccgtgctgt cccaggccac gccgatcatg cgtaccgcgc gcccgtcatc
     2101 gtcgtattcg ccctgcccgc gcgcgctgac cgtgtggatg gtgttgtcgg gccacagcac
     2161 ctggtattcg atctggtagc tgctgcgcgt agccagagca tggtgtgcgg cggccttgac
     2221 ccgctcggca tcggcgggag tgagctgctg gagggtgcgc ttgaaggtgg tggtgccggc
     2281 gccgccgggg attccatgca catgcgccgc gatgtcatcg acgatcatct cgccgctggc
     2341 cgcatcgctt tcccacagtc cgagctgggc cgcggccacg gccagggcaa tgcgctggtg
     2401 cgccagtgcc gcggcggcct gcgtgcgttc acgcaggttg agccagaaca gcaggtagca
     2461 ggacatgatc acgccggcga acagcgagaa catgg
//
LOCUS       DASZZC010000681         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1022224,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000681 DASZZC010000000
VERSION     DASZZC010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022224"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..2558
                     /locus_tag="VHE37_14430"
     CDS             378..2558
                     /locus_tag="VHE37_14430"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HVT36783.1"
                     /translation="MQAAIEARDVPAVARLVIAGSSTRVRQAAAHAIDDPDLLRQLIR
                     EVRGGNDKHVYKVLTSKRDALLEQARRLEQAQAEISAVAQAIERHSRLPYDAIYAPQL
                     DQFESDWKAVTAKAAPRDPELCGRVAQWVARSRETIAGHSRQVAEQAALAQAAAEAAA
                     EARRLADEQAQAAAGQARLLEEQERARAEKQQAEQQALREIGEMVRKARGALSGGGTF
                     RAASVRRTIEDRLAGAPPLPAGLAAQLQQLDQQLAELKDWKSFSVAPKRAELIEAMES
                     LIDAPFDPPALAEKIKNLQEEWRALGKGAAESAQAEADADWQRFQEAGKKAYQPCSEY
                     FAAQALVRQENLQRRDAVLARLMAFEAEQNWESPDWRSVINTLREAKQEWRRHAPVDR
                     RAAKSQEDRFGAVTASLQDRLDAEYARNVKQKEVLIERAGQLLAGDDSRKAIDEVKRL
                     QQKWQAVGPVPREMDQRLWAAFRQQCDAVFNKRQQDSAAHAAGLEHNKVQAIALCEQI
                     EQIALLEGAELVARSGTLAELRKAFESLGEFPRADTRELHHRLERALDRCAKALARQR
                     ARDAERAWDDLFEAANHVRAYRLAFARGREEEAASLKQAAENFMAPIARWPNGGLDAL
                     RQALVSECSGDLEANERALKMVCIRAELLADLPTPAEDQPLRREYQLQRLVQTMGQGV
                     KADAAQLDTLALEWVAAGPVEEAIHAPLLQRFRRCREHGDSRTR"
     gene            2699..4891
                     /locus_tag="VHE37_14435"
     CDS             2699..4891
                     /locus_tag="VHE37_14435"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HVT36784.1"
                     /translation="MSGTAIQVLSNRADLVSGGDALVQVSVPAGTPASAVTVQLNGVD
                     VSSQFTPNDDGTYLGLVSGLVPGVNRIAAQYPGGSSSYLVINHANGGPILAGPQPRPW
                     TCQQGALDAQCNQPPAYSYLYISSDPLKSGFQAYDPENPPADVASTTTDQGVAVPFIV
                     RVETGYQDRDQYRIAALFQPGKPWSAAVPQAQFNHKLLISHGALCGAAYASASTPSVT
                     SYAAPFGLLAGLPVTAGPDSIQYALGAGFAVMSTALDNSGHNCDVVTQAESLIMAKER
                     VVEQYGTLRYTMGSGCSGGSLATQWIANAYPGIYQGIMPTCSFPDAWSTVTQVMDYHL
                     LNLYFGDLTLGPASGGTAGVPWTPVQAAAVEGNMLPVDSVFSDVGFFAGFVPGNTCPG
                     VSAEMVYNAQTNPGGVRCAPADLAINVFGPRPPEAWTDAEKTLDHGFAGLAADNVGVQ
                     YGLSALQQGLITPDMFLDLNARIGGMSIDLQPIANRLSADQPALANAYRSGMINEASN
                     LGSTAIIDCRGPDPGAGHDAYRAFAVRARLDRETGSHANQLIWEGPALIFGDLQCNQN
                     AFAAMDRWLGAVEKDLDDVPLSRKLVQDKPSDLGDACYDGAGQKLSGQLCGDAVVPVN
                     GTPRTAAGDALSTDTNKCQLKPLNRGDDYGPLAFTDAQWTQMQALFPQGVCDFSRKGV
                     SQQPTIPWLTYQDTGGQVIFGGVALPPVPGQSGGGWAGAAFRTFAVEK"
     gene            complement(4914..>5112)
                     /locus_tag="VHE37_14440"
     CDS             complement(4914..>5112)
                     /locus_tag="VHE37_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36785.1"
                     /translation="GTPVTISAVTTHAASCVRSGAWNGNFPRLGGARQVTRQHAGTYQ
                     YFLTCGSVAGSVTRSASVTFE"
BASE COUNT          889 a         1651 c         1776 g          796 t
ORIGIN      
        1 gggttcgacc aacccgcggc gcgggttggc gcgtccgcgc tttgtgcgga cgccccgagc
       61 acagcgaggg gttaggcgcg tatggacgcg cctgactttc ccggtaggag cgccagaata
      121 aaaaatgccc cgcatcgcgg ggcttttcat tttgcccaat cacatcgcta tcgaggcccg
      181 acgcgctgca caggtagaat gccggccggt ccccgttgtg caggtcgaca atgtcgttgt
      241 tctcgcgctt gcttcgaaaa gcgccatccg cttcgtctgt tccggctcca tcgaacccac
      301 cggcggcagc cggaaaaacc gctccccggc aaagcgccga agatcgtgcg ctggcggtgg
      361 ccgccgagga gaaagtcttg caggcggcga tcgaggcccg tgatgtgccg gcggtggcgc
      421 ggctggttat tgcggggtca tcgacccggg tgcgccaggc ggcggcccat gccattgatg
      481 atcctgacct gctgcgccag ctcatccgcg aggtccgcgg cggcaacgac aagcatgtct
      541 acaaggtgct gacgtccaag cgcgatgcgc tgctcgaaca ggcgcgcagg ctcgaacagg
      601 cgcaggccga gatcagtgcg gtggcgcagg cgatcgagcg ccacagccgg ctgccgtacg
      661 acgccatcta cgcgccgcag ctggaccagt tcgagagcga ctggaaagcg gtgacggcca
      721 aggccgcgcc gcgtgatccc gaactgtgcg gcagggtggc gcaatgggtg gcgcgttcgc
      781 gcgagaccat cgctggacac tcgcgccagg tggcagagca ggccgccctt gcacaggctg
      841 ccgccgaagc cgctgcagaa gcacggcgcc tcgccgacga gcaggcacag gcagctgccg
      901 ggcaggcgcg cctgctcgaa gagcaggaac gcgcacgggc cgaaaaacag caggcggagc
      961 agcaggcgtt gcgcgaaatc ggcgagatgg tgcgcaaggc tcgcggcgca ttgagcggcg
     1021 gcggcacgtt tcgcgcagcc agcgtacgcc ggacgatcga ggacaggctg gcgggcgctc
     1081 cgccgttgcc cgccggcctg gcggcgcagt tgcagcagct cgaccagcag cttgccgaac
     1141 tgaaggactg gaaaagtttt tcggtggcgc ccaagcgcgc cgaactgatc gaggcgatgg
     1201 aatcgctcat tgatgcaccg ttcgatccgc cggcgctggc cgagaagatc aagaacctgc
     1261 aggaggagtg gcgcgcgctg ggcaagggcg cggccgaaag cgcgcaagcc gaagccgatg
     1321 ccgactggca acgtttccag gaagcgggga agaaggcata tcagccctgc agcgaatatt
     1381 ttgctgccca ggcgctcgtc cgccaggaga acctgcagcg gcgcgatgcg gtgctcgcca
     1441 ggctgatggc gttcgaagca gagcaaaact gggaaagtcc cgactggcgc tcggtaatca
     1501 acaccctgcg cgaggcgaaa caggaatggc gtcgccatgc gccggtggac cggcgcgccg
     1561 ccaagtcgca ggaggatcgc ttcggcgccg tcactgccag cctgcaggac cggctcgacg
     1621 ccgagtacgc ccgcaacgtg aagcagaagg aagtgctgat cgaacgtgcc gggcagctgc
     1681 tggcgggtga cgacagccgc aaggccatcg acgaggtcaa gcgtctgcag cagaaatggc
     1741 aggccgtggg cccggtgccg cgtgaaatgg atcaacgcct gtgggcggca ttccgccagc
     1801 agtgcgatgc ggtgttcaac aagcgccagc aggattctgc tgcgcatgcg gccgggctcg
     1861 agcacaacaa ggttcaggcc attgcgctat gcgaacagat cgagcagatc gccttgctcg
     1921 aaggagccga actcgttgcg cgcagcggga cgctggcgga attgcgcaag gctttcgagt
     1981 cgctcggcga gttcccgcgc gcggacacgc gtgagttgca tcaccggctg gagcgggcgc
     2041 tggaccgctg cgcaaaggcc ctcgcgcgcc agcgtgcacg cgatgccgaa cgcgcctggg
     2101 atgacctgtt cgaagccgcc aaccacgtgc gtgcgtaccg gcttgccttc gcgcgcggac
     2161 gggaggagga ggctgcttcg ctgaagcagg ccgccgaaaa tttcatggcg cccattgcgc
     2221 gctggcccaa tggcgggctc gatgcgctca ggcaggcgtt ggtcagtgag tgctcgggcg
     2281 atctcgaggc gaatgagcgg gccttgaaga tggtgtgcat ccgcgctgaa ctgctggccg
     2341 acctgccgac gccggcggaa gatcagccgc tgcgccgcga ataccagttg cagcgcctgg
     2401 tgcaaaccat gggccagggt gtgaaagcgg atgcggcgca gctggacacg ctggcgctgg
     2461 aatgggttgc ggccggcccc gtggaggaag cgatccatgc gccgctgctg cagagattcc
     2521 ggcgctgccg cgagcacgga gattcgagga cccggtagct gccgcccgtt gaaggattct
     2581 tcccatgtgt atgcgtatga ccagtcgagt gctgctgtgg atagccctgg cgcttgaact
     2641 cgccgcctgt ggccgcagtt cacccgccgc agatcttggt gccggcggtg cgggccccgt
     2701 gtcggggacc gcaatccagg tgctgtcgaa tcgcgctgac ctggtcagtg gcggcgacgc
     2761 gctggtgcag gtcagcgttc ccgccggcac gccggcctcg gcggtaacgg tgcagctgaa
     2821 cggcgtggat gtcagctcgc agttcacgcc gaacgacgac ggcacctacc tcgggctggt
     2881 cagtggcttg gtgccgggcg tcaaccgcat cgcggcgcag tacccgggcg ggagcagcag
     2941 ctatctggtg atcaaccatg ccaatggcgg cccgatcctc gccgggccgc agccgcggcc
     3001 gtggacctgc cagcagggcg cactcgatgc gcagtgcaac cagcctcccg catattcgta
     3061 tctctacata tcttccgatc cgctcaagtc cgggttccag gcttacgatc ctgaaaatcc
     3121 gcccgccgac gtggccagca ccaccaccga ccagggcgtg gcggttccct tcatcgtgcg
     3181 cgtggaaacc ggctaccagg atcgcgacca gtaccgcatc gcggcgctgt tccagcccgg
     3241 caaaccgtgg agcgccgcgg tgccgcaggc gcagttcaac cacaagctgc tgatctcgca
     3301 tggcgcgctt tgcggtgcag cctatgcatc cgcttccact ccgagcgtga ccagctacgc
     3361 ggcgccattc ggcctgctgg cgggccttcc ggtcaccgcc gggccggaca gcatccagta
     3421 cgcgctcgga gcaggatttg ccgtgatgtc cacggcgctg gacaacagcg ggcacaactg
     3481 cgatgtcgtc acgcaggctg aatcactgat catggccaag gagcgcgtgg tcgagcagta
     3541 cggcacgctg cgctacacga tgggttcggg atgctccggc ggttcgctgg cgacgcaatg
     3601 gattgccaat gcctacccgg gcatctacca gggcatcatg cccacctgct cgtttcccga
     3661 tgcgtggagc acggtcacgc aggtgatgga ttaccacctg ctgaatctct acttcggcga
     3721 cctgacgctg ggcccggcgt ccggcggaac cgcgggcgtg ccgtggacgc cggtgcaggc
     3781 cgcagccgtc gagggcaaca tgctcccggt cgactcggtg ttcagcgacg tcggcttctt
     3841 cgccggcttc gtgccgggca atacctgtcc cggcgtgagc gcggagatgg tgtacaacgc
     3901 gcagaccaat cctggcggcg tgcgttgcgc gccggccgac cttgcgatca acgtgtttgg
     3961 tccgcggccg cctgaagcct ggacggatgc cgagaagacg ctggaccatg gcttcgccgg
     4021 gctggcggcc gacaacgtgg gtgtgcagta cgggctgagt gccctgcagc agggcctgat
     4081 cacgccggac atgttcctcg atctcaacgc caggatcggc ggcatgagca tcgacctgca
     4141 gccgatcgcc aaccggctca gcgccgatca gccggcgctg gccaatgcgt atcgcagcgg
     4201 catgatcaac gaggccagca atctcggcag cacggcgatc atcgactgcc gcggtcctga
     4261 tccgggcgcg ggccacgacg cctaccgggc ttttgccgtt cgcgcgcgcc tggaccggga
     4321 gaccggctcg cacgccaacc agctgatctg ggaaggcccg gcactcatct tcggcgacct
     4381 gcagtgcaac cagaacgcgt tcgctgcaat ggatcgctgg ctgggggccg tcgagaaaga
     4441 tctggatgac gtgccgctgt cgcgcaagct ggtgcaggac aagccgtcag acctgggcga
     4501 cgcctgctac gacggcgccg ggcagaagtt gtccggccag ctctgcggtg atgcggtggt
     4561 gccggtgaat ggcactccgc gcaccgcggc cggcgatgcc ctcagcaccg ataccaacaa
     4621 gtgccagctg aagccgctca accgcggcga cgactacggg ccgctggcct tcaccgatgc
     4681 gcagtggaca cagatgcagg cgttgtttcc gcagggcgtc tgcgatttca gcaggaaggg
     4741 tgtgagccag cagccgacca ttccctggct gacctaccag gacacgggtg gccaggtgat
     4801 cttcggcggc gttgccttgc cgccggtgcc gggccagtca ggcggcggct gggccggtgc
     4861 tgcgttccgc acattcgccg tcgaaaaata ggcggcggca gccgcgccgc cggctactcg
     4921 aaggtcacgc tcgccgagcg cgtcacgctg cccgcaacgc tgccgcaggt gaggaaatac
     4981 tggtacgtcc ccgcgtgctg tcgcgttacc tggcgtgcgc cgcccaggcg cgggaaattg
     5041 ccattccacg caccgctgcg gacgcaactg gcagcgtgcg tggtcaccgc gctgatcgtc
     5101 accggcgtgc cg
//
LOCUS       DASZZC010000682         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1022303,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000682 DASZZC010000000
VERSION     DASZZC010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022303"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VHE37_14445"
     CDS             complement(<1..1551)
                     /locus_tag="VHE37_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36786.1"
                     /translation="MNQPRPRSLTADASAWLPFTSGPARLSLCGHRGALQMDFDLAQT
                     QGFVVARRPCTMKLPDDFVVRLRARGAGGRNHLELKLVDGAGTSVWRCVWRDLRPSPR
                     WKRLGADAGMFIFAWGPRGGGPIADVSAIEIAVVAGAGGAGRIDIDELEILPRATRPW
                     LAAASSAQAGHQAAAVSTTAGWKPAVADTAPWLELDGGGLRPAASWMIEWTGAPPDSG
                     LRVRLDRGRGWSKGFAVPAPVGERSYLLLPAGKVRRIRLELPPRCAGLRLRELPASCA
                     RSPEDLWHAIAAMEPRGLLPRWLLREQSLWTPVGRVAGGGCALMSEQGMVEIAPGSCS
                     LEPMLRVDGQLRSWADAAVTQDLSEHWQPLPKVTWQLPGLVLEISAQPAAGGALALRY
                     RLKNSAAAAARVQLLLLLRPLQVTPEWQKSGSHGGVAPVHRLQWRAGRLRVDERIELA
                     PATRPDRYQAWTLAQGVLPSAVAAPQPAAARDRHGSLSASLLYQLTLEPGASAECVVD
                     CASLAAAPA"
     gene            1667..4399
                     /locus_tag="VHE37_14450"
     CDS             1667..4399
                     /locus_tag="VHE37_14450"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HVT36787.1"
                     /translation="MPRTFALLLPLLLLAGPALSAQAPLRGVCGTAFDRPGALGTLRD
                     GRVLYPAGRLTAVGQFPAGGALAPDGRHYWSVSAGRGANDVRIVDLASGEVVQVLPLP
                     GASGAMVFSADGRRAYVSGEPRGDSLPGGPTLADAGDAIHVFAVDPADGHAVEREPLL
                     LPRTAGGHGRETGIPPNPQLPSLPAGIAISPDGQTLLVALHNADLAAVIDLRDGSTRL
                     VPTGAYPQAAAIERSGRYGYIGNLLDGSLTRIDLARAEVVDTFHGLGGSAGDFNAHPQ
                     ALLADAHADLLYVAVTDRDSIMAVDTRSGALTPLISLRQHAHYGAAPVALALSANGRT
                     LYAAAAGLNAIAQIRLQRGAHLRATLGGLITTADYPQDVQISGDGCTLVWAAARGLGS
                     RPNAERGTPWFDRSVPPRGSSLLEQFAGQVGVLPVPDAHALATLTALARDGLRVPGDA
                     PARSVIPAPGAVSPIQHVFLVIRENRTYDQIFGSIARADGAAALQVAEDNCGAANPQF
                     EGNDRAHPGCGATPNAHALARRFPLLDRFYENSEVSADGHLITTGAYAIDHVLKGLLG
                     EYAARGRAVEENLFPIDFPPRQFLFDQAVRSGVSLRIYGERSGGIIAGRTPDRADTYD
                     AVQDAVEHDYPAMIGCRPHAISIPDTVDCVFDAGLGAAPPHARSRIDVFARRFREQLA
                     AGAVPRFNYLALPNDHTAGTVPGGRTPLAMLADNDLALGQLVQLVSHSAVWHDSAIFV
                     MEDDSQDGPDHVDAHRGPVLVISPWARHGGAVVHTRYDQYSMLRTIELILGLPPLSVH
                     DAGAAPMYDAFALKPDDSPYDAIAPEQSLLAVNAPHAANAQLSQALPFEREDAVPQQL
                     SDLILWQSVFGPLSPPPAPGPHGSAAERQRAEDALLALRNQKQMELPAADDDDD"
     gene            4715..>5394
                     /locus_tag="VHE37_14455"
     CDS             4715..>5394
                     /locus_tag="VHE37_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT36788.1"
                     /translation="MNRIQEAMRSTITAARPLSTALLLAAAALAPLRAHAVDAVTGRS
                     LYNNSVGPAGSTCSNSNCHGTNPASGANKIRNGANNAAAIQRGISANSGGMGAYNGKL
                     TATDLSNIAGFLGITAAVSPLTLSFPSTAVGTSSAGQTVTVTSTGVSALSVSSLSSNS
                     ADFVIGSTGTCKAGSSVPGFSTSTAKYSNCSVVISFAPQAAGTRSGTLTIATSDPAQP
                     SVAIALSG"
BASE COUNT          804 a         2005 c         1810 g          775 t
ORIGIN      
        1 cgccggcgca gcagcgagcg atgcgcaatc gacgacgcac tcggcgcttg cgcccggttc
       61 gagcgtgagt tggtaaagca gcgaggccga caggctgccg tgacgatcgc gcgccgcggc
      121 aggctgcggc gctgccacgg ccgacggcag taccccctgc gccagggtcc atgcctggta
      181 gcgatccggc cgggtggcgg gcgcaagttc gatgcgctca tcgacccgca ggcgtcccgc
      241 ccgccactgc aggcgatgta ccggcgccac gccgccatgg ctgccggact tctgccactc
      301 cggcgttacc tgcagcggcc gcagcagcaa cagcaattgc acgcgtgcgg ccgccgccgc
      361 ggaatttttc agccggtagc gcagcgcgag tgcaccaccg gctgcaggct gcgcagaaat
      421 ttccagcacc aacccgggca actgccaagt caccttcggc agcggctgcc aatgctccga
      481 cagatcctgc gtgaccgcgg cgtcggccca gctgcgcagt tgcccgtcga cgcgcagcat
      541 gggttcgagc gagcaggagc ccggcgcgat ttccaccatg ccctgctcgc tcatcagcgc
      601 acagccgccg cccgcgacgc ggcccaccgg cgtccataaa ctctgctcgc gcagcagcca
      661 gcgcggcagc aagccgcgcg gttccatggc ggcgatggcg tgccagagat cttcgggtga
      721 gcgcgcgcag gatgccggca gttcgcgcag gcgcaggccc gcacagcgcg gcggcaactc
      781 cagccggatg cgccgcactt ttccggccgg cagcagcagg tagctgcgct cgcccaccgg
      841 cgcgggcacg gcgaaaccct tcgaccagcc gcgcccgcgg tcgaggcgca cgcgcaggcc
      901 cgaatctggc ggcgcaccgg tccattcgat catccagctg gccgctggcc gcaggccgcc
      961 gccgtccagt tccagccacg gtgcggtgtc ggccacggcc ggcttccagc ccgcggtggt
     1021 cgacactgcg gcagcctggt gcccggcttg cgccgatgag gcggccgcca gccacggccg
     1081 cgtggcccgc ggcaggattt ccagttcatc gatgtcaatg cggcccgcgc cgcccgcacc
     1141 cgccaccacc gcgatctcga ttgcgctgac atcggcgatg gggccgccac cgcgcgggcc
     1201 ccaggcgaag atgaacatgc cggcgtcggc gcccagcctc ttccagcgcg gcgacggacg
     1261 caggtcgcgc catacgcagc gccacacgct ggtgccggcg ccatcgacca gtttcagttc
     1321 caggtgattg cgcccgccgg cgccgcgcgc gcgcaggcgc accacgaagt cgtcgggcag
     1381 cttcatcgtg cacggccggc gcgcgacgac gaagccctgc gtctgcgcca ggtcgaaatc
     1441 catctgcagc gcaccgcggt gcccgcacag cgacagccgc gctggccccg aagtgaacgg
     1501 cagccaggcg gaagcgtccg cggtgagcga tcgtgggcgg ggctgattca tgagctgggc
     1561 atctcgctgg gcgcagtgtg ggcgatgcga ggcccgggcc ggttgacatg aatcaatgag
     1621 cgcccggcgc ggcggcgcca gcatgtgccc gaccgatcac aggcccatgc cccgtacctt
     1681 tgccttgctg ctgccgctgc tgctgctcgc gggccccgcg ctgtccgcgc aggccccgct
     1741 gcgcggtgtg tgcggcacgg cattcgaccg gccgggcgcg ctcggcacgc tgcgcgacgg
     1801 ccgcgtgctg tatcccgccg ggcgcctgac cgccgtcggc cagtttcccg cgggcggcgc
     1861 gctcgcgccc gatggccggc attactggtc ggtctcggcg ggccgcggcg ccaacgacgt
     1921 gcgcatcgtc gatctggcca gcggcgaagt ggtgcaggtg ttgccgctgc ccggcgcctc
     1981 gggcgccatg gtgttttccg ccgatggccg ccgggcctat gtctccggcg agccgcgcgg
     2041 cgacagcctg cccggcgggc ccacgctggc cgatgccggt gatgccatcc acgtgttcgc
     2101 cgtggaccct gccgatggcc acgccgtgga acgggagccg ctcctgctgc cgcgcacggc
     2161 cggcggccac ggccgcgaga ccggcatacc gcccaacccg cagctgccgt cgctgccggc
     2221 gggcatcgcc atttcgcccg atggccagac cttgctggtg gccctgcaca acgccgacct
     2281 cgcggcggtg atcgacctgc gcgacggcag cacgcggctg gtgcccaccg gcgcgtatcc
     2341 gcaggcggca gcgatcgagc gcagcggccg ctacggctac atcggcaacc tgctcgacgg
     2401 ttcgctgacg cgcatcgacc tggcgcgggc ggaagtggtc gacacgttcc atggcctcgg
     2461 cggcagcgcg ggcgatttca acgcgcatcc gcaggcgctg ctggcggatg cgcatgcgga
     2521 cctgctctac gtggccgtca ccgaccgcga ctcgatcatg gcggtggaca cgcgcagcgg
     2581 cgcgctgacg ccattgatct cgctgcggca gcacgcgcac tacggggccg cgccagtggc
     2641 cctggcgctg tcggccaatg gccgcacact ctatgccgcg gcggcggggc tcaacgccat
     2701 cgctcagatc cgcctgcagc gcggtgcgca tttgcgcgcg acgctgggcg ggctgatcac
     2761 gactgccgac tatccgcagg acgtgcagat cagcggtgac ggttgcacgc tggtgtgggc
     2821 cgccgcgcgc gggctgggct cgcgccccaa cgccgagcgc ggcacgccgt ggttcgatcg
     2881 cagcgtgcca ccgcgcggca gctcgctgct ggagcaattc gccggccagg tcggcgtgtt
     2941 gccggtgccg gatgcgcacg cgctggcgac gctcaccgcg ctggcacgcg atggactgcg
     3001 cgtgccgggc gatgcgcctg cgcggtcggt cataccggcg ccgggtgcgg tatcgccgat
     3061 ccagcatgtc ttcctggtga tccgcgaaaa ccgcacctat gaccagatct tcggctccat
     3121 cgcgcgcgcc gacggcgccg ccgcgctgca ggtagcggag gacaactgcg gcgcggccaa
     3181 cccgcagttc gaaggcaatg accgcgcaca tcccggctgc ggcgccacgc ccaacgcgca
     3241 tgccctggcc cggcgcttcc cgctgctcga ccgcttctac gaaaacagcg aggtctccgc
     3301 cgacggccac ctgatcacca ccggcgccta tgccatcgac cacgtgctca aggggctgct
     3361 gggcgaatac gcggcgcgcg gacgcgcggt ggaggagaac ctgttcccta tcgattttcc
     3421 gccgcgccag ttcctgttcg accaggcggt gcgctcgggt gtgtcgctgc gcatctacgg
     3481 cgagcgcagc ggcggcatca ttgccgggcg cacgcccgac cgcgccgaca cctacgacgc
     3541 cgtgcaggac gcggtggagc acgactaccc ggcgatgatc ggctgccgtc cgcatgcgat
     3601 ctcgattccc gacacggtcg attgcgtgtt cgacgccggc ctgggcgccg caccgccgca
     3661 tgcgcgctcg cgcatcgacg tgttcgcgcg ccgcttccgc gagcagctcg ccgccggcgc
     3721 ggtgccgcgc ttcaactacc tggcgctgcc caacgaccac accgccggca ccgtgcccgg
     3781 cggtcgcacg ccgctggcca tgctggccga caacgacctt gcgctcggcc agctggtgca
     3841 gctggtctcg cacagcgccg tctggcatga cagcgccatc ttcgtgatgg aggacgacag
     3901 ccaggacggc cccgaccacg tcgacgcgca ccgcggaccc gtgctggtga tctcgccgtg
     3961 ggcgcgccac ggcggcgcgg tggtgcacac gcgctacgac cagtactcga tgctgcgcac
     4021 catcgagctg atcctgggcc tgccgccgct gtcggtgcac gacgccggcg ccgcgccgat
     4081 gtacgacgcc tttgcgctga agcccgacga ctcgccgtac gacgccattg cccccgagca
     4141 gtcgctgctc gccgtcaacg cgccgcacgc ggccaatgcg caactgtcgc aggcactgcc
     4201 gtttgagcgc gaagacgcgg tgccgcagca gttgtccgac ctgatcctgt ggcagtcggt
     4261 gttcggcccg ctgtcgccgc cgccggcgcc cggcccgcac ggctcggcgg ccgagcgcca
     4321 acgcgcggag gacgcgctgc tcgcgctacg aaatcagaag cagatggaac tgccggccgc
     4381 cgacgatgac gatgactgag acctggaaaa aaacagccac cgcaatgctt tggttgatgt
     4441 tattaattat atagtttttt ttattttgaa taataattaa taacatcacc tggtgacttg
     4501 cggtgacacc tccagcgcgc gtgcaggcgg ctctgctacg gatttcgtag cacgcaggca
     4561 cgacttgctg cccgccagct gaatcgcccg gcaactgcgc ggccgttcag ggggccgcca
     4621 cgcgccgctt gtccacaact caatagaaac gaggctccag cagccggtcc ataagtttgt
     4681 cttaagactc gcttcctaac ctgccgcgca tgtcgtgaac cggatccagg aagccatgcg
     4741 ttcaaccatc accgctgcac gcccgttgtc caccgcgctg ctgctcgccg ccgcggcgct
     4801 ggcgccgctg cgcgcgcacg cggtggacgc ggtgaccggc cgctcgctgt acaacaactc
     4861 ggtcgggccc gcgggcagca cctgctccaa ctcaaactgc cacggcacca acccggcatc
     4921 gggcgccaac aagatccgca acggcgccaa caacgcggcg gcgatccagc gcggcatctc
     4981 cgccaactcc ggcggcatgg gcgcctacaa cggcaagctg accgcgaccg acctgtcgaa
     5041 catcgccggc ttcctcggta tcaccgcggc ggtctcgccg ctcacgctga gtttcccctc
     5101 gaccgcggtg ggcaccagca gcgccggcca gacagtcacc gtcaccagca ccggcgtgtc
     5161 ggcgctgtca gtgagttcgc tcagcagcaa ctccgccgat ttcgtcatcg gcagcaccgg
     5221 cacgtgcaag gccggcagca gcgtgcccgg cttctccacc agcacggcga aatacagcaa
     5281 ctgctcggtg gtgatcagct tcgcgccgca ggccgccggc acgcgcagcg gcacgctgac
     5341 catcgccacc agcgatcctg cgcagccgag cgtcgccatc gcgctcagcg gcag
//
LOCUS       DASZZC010000683         5930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1270882,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000683 DASZZC010000000
VERSION     DASZZC010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270882"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VHE37_14460"
     CDS             <1..233
                     /locus_tag="VHE37_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36789.1"
                     /translation="AKMVEGKVRKFAAEITLLGQEFVRNAEYGMKSGDTIEALLKAKN
                     AKVARFTRFAVGDGIEKAQTDFAAEVAAMAKG"
     gene            323..1045
                     /gene="pyrH"
                     /locus_tag="VHE37_14465"
     CDS             323..1045
                     /gene="pyrH"
                     /locus_tag="VHE37_14465"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HVT36790.1"
                     /translation="MNAKPAYKRILLKLSGEALMGDLGFGISPDVMSFMAAELKDLVG
                     AGVEVALVVGGGNIFRGEGLARAGMDRVTGDQMGMLATVINALAIQDAIERIGLEARV
                     QSALRINEVCEDFIRRKAVRHLEKGRVVVFAAGTGNPFFTTDSAAALRAVEIEADLML
                     KATKVDGIYTADPKKDRSAKKYERISYDEALDRRLSIMDQTAMVLCRDHAMKLRVYDM
                     SAAGALRKIVMGDESIGTHVYV"
     gene            1063..1626
                     /gene="frr"
                     /locus_tag="VHE37_14470"
     CDS             1063..1626
                     /gene="frr"
                     /locus_tag="VHE37_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVT36791.1"
                     /translation="MADLNKIKSDATARMGKCLDTLKTQLSKLRTGRANAGLLDHVQV
                     DYYGSEVPLSQAASVVVEDARTISITPWDKGMVPVIEKAIMKSDLGLNPNTAGNIIRI
                     IMPALTEERRRDLTKVVKGEAETARVAIRNVRRDANQAVKDLGKAKTISTDEEKRGEA
                     EIQKLTDQWVAKVDETAAAKEKELMSM"
     gene            1752..2471
                     /gene="uppS"
                     /locus_tag="VHE37_14475"
     CDS             1752..2471
                     /gene="uppS"
                     /locus_tag="VHE37_14475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HVT36792.1"
                     /translation="MTQSPPKLAAATVPHHVAVIMDGNGRWAQQRGQPRAMGHRAGVK
                     SVRRLLRAAHEHGVGVVTVYAFSQENWRRPEEEVSLLMQLFIKTLAREINAMHRNGIR
                     IRFIGEHSMFEPLLRAEMRRAEKLTALNDGLTFVIAVGYGGQWDIVQAAQQLAEKHKP
                     ITAESLEKHLDTTGLPHPDLLIRTGGEKRISNFLLWQLAYTELYFSDVLWPDFGDAEF
                     GEAIRWYSSRQRRFGRVPESA"
     gene            2770..3606
                     /locus_tag="VHE37_14480"
     CDS             2770..3606
                     /locus_tag="VHE37_14480"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVT36793.1"
                     /translation="MLLQRVVTALVLLPLLLAAVWWAPTPWLYALFCAVAMVMAWEWS
                     ALMKWTQPARRWSYLAISAVLLAAAWLIPQRELLLPWLLGLAALWWIAAVLILARYPD
                     NFAQRPPADWWMAPLGVLMMLSTMLSLARLHAMPAMHPHGGALKLLYFFFIVFCADTG
                     AYFAGRAFGKHKLAPKVSPGKTVEGAIGGFALVALWAATAGVWVFDVHGADTALLVVL
                     SLLVAVISIVGDLTESMFKRIAGVKDSGTILPGHGGILDRVDSILAAAPVMVLGLLLT
                     GL"
     gene            3609..4799
                     /locus_tag="VHE37_14485"
     CDS             3609..4799
                     /locus_tag="VHE37_14485"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HVT36794.1"
                     /translation="MHNLVVLGATGTVGRNTLDVARRHADKLTVIALTAHRDVEGLAV
                     LCREFLPRVAVVADAAAAQALRERLSGMVIDIAHGAQALADAAGLPEAQQVMSAIVGA
                     AGLVPTLAAVRAGKRVLIANKEPLVMAGALLMAEAQKSGAEIIPVDSEHNAIFQCLPH
                     GARCGETARGVRRLVLTASGGPFREFTAEQMARVTPAQAVKHPNWVMGPKISVDSASL
                     MNKGLELIEAHILYRLPPQQLDVVIHPESIVHSLVEYVDGSLLAQLSSPDMRVPIAHA
                     LAHPQRWESGAAPLDLAALARLNFSAPDETRFPCLTLARQALAAGGNAPNVLNAANEV
                     AVEAFLAGRLGFGEIAQLIEHCLSAAEMPHARDLEAILAVDGQARQVAARRLQSSERR
                     VARV"
     gene            4792..>5930
                     /gene="rseP"
                     /locus_tag="VHE37_14490"
     CDS             4792..>5930
                     /gene="rseP"
                     /locus_tag="VHE37_14490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HVT36795.1"
                     /translation="MSEVFWNVGCYLFAIAILVGFHEFGHYWVARRCGVKVLRFSIGF
                     GKVLWSRRAADGVEWSLSAIPLGGYVKMLDEREGEVAPAERHLAFNNATVLRRSLIVA
                     AGPVFNFLLAIAFYWLVFVIGAEGIKPMLAEPPAQSAAARAGLHEGEQVMRVDGRSVI
                     TWTELRTELISHALDGGSMQLDVQGRDGAQRSVKLDLSEVRVDPAVLFDDLGLEIYQP
                     PVPARVGDLAPGEAAASAGIHAGDLLLTRNGEPVGNWRDWTKWVREHAGEVVQIGIER
                     DGQHLNVPVIIGRTQLDGKTVGRFGAYNAPMPRELWQNLRAQTRLGALEAVPAAAIHT
                     WRMSKLTARLLFLMVRGDVSIKNISGPINTAQAVGTAAHFGLVPFL"
BASE COUNT          975 a         1952 c         2044 g          959 t
ORIGIN      
        1 tggccaagat ggtcgagggc aaggtgcgca agttcgccgc cgaaatcacc ctgctgggcc
       61 aggagttcgt gcgcaacgcc gagtacggca tgaagtccgg cgacaccatc gaggcgctgc
      121 tcaaggccaa gaacgccaag gtggcgcgct tcacccgctt cgcggtgggt gacggcatcg
      181 agaaggcgca gaccgacttc gcggcggaag tcgccgccat ggccaaaggc tgaagggcct
      241 ttaaggtaga atcttcagag tcgaacacag gccccggaaa cggggcctct tttcaggtgc
      301 agcacgccat ccggagagtc acgtgaacgc gaagccggcc tacaagcgca tcctcctcaa
      361 gctttcgggc gaggcgttga tgggcgacct gggtttcggc atctcgcccg atgtcatgtc
      421 gttcatggcg gcggaactca aggacctggt cggcgccggc gtcgaggtgg cgctggtggt
      481 cggcggcggc aacatcttcc gcggcgaagg cttggcgcga gccggcatgg accgcgtgac
      541 cggcgaccag atgggcatgc tggccacggt gatcaacgcg ctggccatcc aggacgcgat
      601 cgagcgcatc gggctggaag cgcgcgtgca gtcggcgctg cgcatcaacg aagtctgcga
      661 ggatttcatc cggcgcaagg cggtgcggca ccttgagaag ggccgcgtgg tggtgtttgc
      721 cgccggcacc ggcaacccgt ttttcaccac cgattcggcg gcggcgctgc gcgcggtgga
      781 gatcgaggcc gacctgatgc tgaaggcgac caaggttgac ggcatctaca ccgccgatcc
      841 gaagaaggac aggagcgcga agaaatacga gcgcatcagc tacgacgagg cgctggaccg
      901 gcgcctgtcg atcatggacc agaccgccat ggtgctgtgc cgcgaccacg ccatgaagct
      961 gcgcgtgtac gacatgagcg ccgccggcgc gctgaggaaa atcgtcatgg gtgacgagtc
     1021 catcggcacc cacgtttacg tctgagcaaa aaggaaaccg acatggccga cctcaacaag
     1081 atcaagtccg acgccaccgc gcgcatgggc aaatgcctgg acacgctcaa gacgcagctg
     1141 tcgaagctgc gcaccggccg cgccaacgcg ggcctgctcg accatgtgca ggtggactac
     1201 tacggctcgg aagtaccgct gtcgcaggcg gccagcgtgg tggtggagga tgcgcgcacc
     1261 atctcgatca cgccgtggga caagggcatg gtgccggtga tcgagaaggc gatcatgaag
     1321 tccgacctcg ggctcaaccc caacaccgcc ggcaacatca tccgcatcat catgccggcg
     1381 ctgaccgagg agcgccgccg cgacctgacc aaggtggtca agggcgaggc cgagaccgcc
     1441 cgcgtggcca tccgcaacgt gcgccgcgat gccaaccagg cggtgaagga cctgggcaag
     1501 gccaagacca tcagcaccga cgaagagaag cgcggcgagg ccgagatcca gaagctcacc
     1561 gaccagtggg tggccaaggt cgacgaaacc gctgcggcca aggaaaagga actgatgtcg
     1621 atgtgagccg gcgcgcggcc cgcttagaca agaacgactg ctgcacggtt tttccgcgcc
     1681 ggctgcacga aaattccgcg aacttccgct agcgtttcag tctctcgcct ccgagcccac
     1741 ctggactttc cgtgacccag tctccgccca agctggccgc cgccaccgtc ccgcatcacg
     1801 tcgccgtgat catggacggc aacggccgct gggcacagca gcgcggacag ccgcgcgcca
     1861 tgggccatcg ggccggcgtc aagagcgtgc ggcgcctgtt gcgcgccgcg cacgagcacg
     1921 gcgtgggcgt ggtcacggtg tacgcgttct cgcaggagaa ctggcgccgg ccggaagagg
     1981 aagtcagcct gctgatgcag ctgttcatca agacgctggc gcgcgagatc aacgccatgc
     2041 accgcaacgg catccgcatc cgcttcatcg gcgagcacag catgttcgag ccgctgctgc
     2101 gtgccgagat gcgccgtgcc gagaagctca ccgcgctcaa cgacggcctc accttcgtca
     2161 tcgcggtcgg ctacggtggg cagtgggaca tcgtgcaggc cgcgcagcag ctggcggaaa
     2221 agcacaagcc catcaccgcc gagtcgctgg aaaagcacct ggacaccacc ggcctgccgc
     2281 acccggacct gctgatccgc accggcggcg agaagcgcat cagcaatttc ctgctgtggc
     2341 agctggccta caccgagctg tatttcagcg acgtgctgtg gccggatttc ggcgatgccg
     2401 aattcggcga ggccatccgc tggtactcct cgaggcagag acgcttcggg cgggtgcctg
     2461 aatccgccta agcgcggtgc gcctgttgcg ctttgccccg tacccggcgc cgctcccgcg
     2521 gctcgcatcc ccgctggagc ggggcccctg ctcgtacgct cgcgtcgctg gtactcctcc
     2581 aggcagagac gcttcgggcg ggtgcccgag agcgcgtgaa ccctccaccg caccttgttc
     2641 tggacggtta taaattattt atcgatttta attttgatcg ataatattta acaacaaaac
     2701 agagggtgcg gttagcaaaa aacggcgctt tcccggacaa ttcacgcccg gtttttccgc
     2761 tgatccgaga tgctgctcca acgcgtcgtc accgccctgg tcctgctgcc gctgctgctc
     2821 gccgccgtgt ggtgggcgcc cacgccctgg ctctatgcgc tgttctgcgc ggtggcgatg
     2881 gtgatggcct gggaatggag cgcgctgatg aagtggaccc agcccgcgcg ccgctggagc
     2941 tacctggcga tcagcgcggt actgctggcc gccgcctggc tgattccgca gcgcgagctg
     3001 ctgctgccct ggctgctggg cctggctgcg ctgtggtgga ttgccgccgt gctgatcctg
     3061 gcgcgctacc cggacaactt cgcgcagcgc ccgcccgcgg actggtggat ggcgccgctg
     3121 ggtgtgctga tgatgctgtc gaccatgctg tcgctggcgc gcctgcatgc catgccggcg
     3181 atgcacccgc acggcggcgc gctgaagctg ctgtacttct tcttcatcgt gttttgcgcc
     3241 gacaccggcg cgtatttcgc cggccgtgct ttcggcaagc acaagctggc gccgaaagtg
     3301 tcgccgggca agacggtgga aggcgccatc ggcggcttcg cgctggtggc gctgtgggcc
     3361 gccaccgccg gcgtctgggt attcgacgtg cacggcgccg acactgcgct gctcgtggtg
     3421 ctgtcgctgc tggtggcggt gatctccatc gtcggcgacc tcaccgagtc gatgttcaag
     3481 cgcatcgccg gcgtcaagga cagcggcacc atcctgcccg ggcacggcgg catcctcgac
     3541 cgcgtcgaca gcatcctcgc cgccgcgccg gtgatggtgc tgggcctgct gctgaccgga
     3601 ctgtaagcgt gcacaacctc gtcgtcctcg gcgccaccgg cacggtcggc cgcaacacgc
     3661 tcgacgtggc acgtcgccac gcggacaagc tgacggtcat cgcgctcacc gcgcaccgcg
     3721 acgtcgaagg actcgcagtc ctgtgccgcg agtttctacc tcgcgtggcg gtagtggcgg
     3781 atgcggccgc ggcacaggcg ctgcgcgagc ggttgtccgg catggtcatc gacatcgcgc
     3841 acggcgcgca ggcgctggcc gatgcggcgg ggctgccgga ggcgcagcag gtgatgtccg
     3901 ccatcgtcgg cgccgcgggc ctcgtgccta cgctggcggc ggtgcgcgcg ggcaagcgcg
     3961 tgctcatcgc caacaaggag ccgctggtga tggccggcgc gctgctgatg gccgaagcgc
     4021 agaaatccgg cgccgagatc attccggtgg attccgagca caacgccatt ttccagtgcc
     4081 tgccgcacgg cgcgcgctgc ggcgagactg cgcgcggcgt tcgccgcctg gtgctcaccg
     4141 cttccggcgg gccgttccgc gaattcaccg ccgagcagat ggcgcgcgtc acgccggcgc
     4201 aggcggtgaa gcatcccaac tgggtgatgg gcccgaagat ctcggtggat tccgccagcc
     4261 tgatgaacaa gggcctggag ctgatcgagg cgcatatcct gtatcgcctg ccgccgcagc
     4321 agctggacgt ggtgatccat cccgagagca tcgtccactc gctggtcgaa tacgtggacg
     4381 gctcgctgct ggcccagctg tcgtcgcccg acatgcgcgt gccgatcgct catgcgctgg
     4441 cccatccgca gcgctgggag tccggcgcgg cgccgctgga cctcgcggcg ctggcccggc
     4501 tgaatttttc cgcgccggac gaaacgcgct tcccatgcct cacgctggcg cggcaggcgc
     4561 tggcggccgg cggcaacgcg ccgaatgtat tgaacgcagc caacgaagtc gctgtcgaag
     4621 cgttcctggc cggccggctg ggctttgggg agatcgctca gctgatcgaa cattgcctgt
     4681 cggcggccga gatgccgcac gcgcgtgacc tggaagcgat actggcggtg gatggccagg
     4741 cgcgccaggt cgcggcgcgc cggctgcagt cgagtgagag gagagtcgcc cgtgtctgaa
     4801 gtgttctgga atgtcggctg ttacctgttc gccatcgcca tcctggtcgg cttccatgag
     4861 ttcggccact actgggtggc gcgccgctgc ggcgtcaagg tgctgcgctt ctccatcggc
     4921 ttcggcaagg tgctgtggag ccgccgcgcg gccgacggcg tggagtggag cctgtcggcc
     4981 atcccgctgg gcggctacgt caagatgctc gacgagcgcg agggtgaggt ggcgccggcc
     5041 gagcggcacc tggccttcaa caatgccacg gtgctgcgcc gcagcctgat cgtcgccgcc
     5101 gggccggtgt tcaatttcct gctggccatc gctttctact ggctggtgtt cgtcatcggc
     5161 gccgagggca tcaagccgat gctggccgag ccgccggcgc agtcggctgc cgcgcgcgcc
     5221 ggcctgcatg aaggcgagca ggtgatgcgc gtcgacggcc gcagcgtgat cacctggacc
     5281 gagctgcgca ccgagctgat cagccacgcg ctcgacggcg gcagcatgca gctggacgtg
     5341 cagggccgcg acggcgcgca gcgcagcgtg aagctggacc tgtcggaagt gcgcgtcgat
     5401 ccggcggtgc tgttcgatga tctcgggctc gagatctacc agccgccggt gccggcgcgc
     5461 gtcggcgacc tggcgcccgg cgaagcggcg gccagcgccg gcatccatgc cggcgacctg
     5521 ctgctcacac gcaacggcga gccggtcggc aactggcgcg actggaccaa gtgggtgcgc
     5581 gagcacgccg gcgaagtggt gcagatcggg atcgagcgcg acggccagca cctgaatgtg
     5641 ccggtgatca tcggccgcac ccagctcgac ggcaaaaccg tgggccgttt cggcgcctac
     5701 aacgctccga tgcccaggga gttgtggcag aatctgcgcg cccaaacgcg cctcggcgcg
     5761 cttgaggcgg tgccggcggc ggcgatccac acgtggcgca tgtcgaagct caccgcccgg
     5821 ctgctgttcc tgatggtgcg cggcgatgtg tcgatcaaga acatcagcgg ccccatcaac
     5881 acggcgcagg cagtcggcac cgcggcgcat ttcggcctgg tgccgtttct
//
LOCUS       DASZZC010000684         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1022448,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000684 DASZZC010000000
VERSION     DASZZC010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022448"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /gene="kdpB"
                     /locus_tag="VHE37_14495"
     CDS             complement(<1..1686)
                     /gene="kdpB"
                     /locus_tag="VHE37_14495"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HVT36796.1"
                     /translation="MTSNRKSSSPFDRALLGPAIRDSFVKLDPRTLIRNPVIFITALV
                     SLLATAILLRDLLEGRGQLLFSGQVVLWLWFTVLFANFSEAIAEGRGKAQAESLRKTR
                     TQTIAHVLVSPDSPNSQATRSVPASQLKVGDVAMVKAGEVIPGDGDVIMGVATVDESA
                     ITGESAPVIREAGGDRSAVTGGTRVLSDHILVRITATAGGSFLDRMIALIEGASRQKT
                     PNEIALNILLAGMSLIFVFATATIPAFALYAGGNVGVIVLVALFVTLIPTTIGALLSA
                     IGIAGMDRLVRFNVLATSGRSVEAAGDVDVLLLDKTGTITLGNRQASDFIPLVGVTTA
                     ELADAAQLASLSDETPEGRSIVVLAKEKYALRGREVAPLHARFVPFSAQTRMSGVDVD
                     GHSIRKGAVEAIIEHLSKMGRSGAVKELKEIADGISRSGGTPLAVAKDNRALGVIHLK
                     DIVKGGIRERFAELRRMGIRTVMITGDNPLTAAAIAAESGVDDFLAQAKPEDKLALIR
                     KEQAEGRLVAMCGDGTNDAPALAQADVGVAMQTGTQAAREAGNMVDLDSDPTKL"
     gene            complement(1697..>3154)
                     /gene="kdpA"
                     /locus_tag="VHE37_14500"
     CDS             complement(1697..>3154)
                     /gene="kdpA"
                     /locus_tag="VHE37_14500"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HVT36797.1"
                     /translation="LPLNTPHFANIGADGAFNTAVSFTTNTNWQWYSGEAVLSNLSQM
                     FALVIHNFMSAATGIALAFAFFRGFARRETSTIGNFWADATRITLYVLLPICVVYTIF
                     LIASGVPQTLTQQIDATTLEGARQTITLGPVASQEAIKMLGTNGGGVFNANSAHPYEN
                     PTALSDLLELLSILVISAGLTNTFGRMVKNPLQGWVLYSAMSVLFLAGALACYAAEAG
                     GNPAFDAMGIDQHASTLQAGGNMEGKEARFGIADSVLWATATTDASNGSVNSMHDSYT
                     PLGGLIPMLNIMLGEVIYGGVGSGLYGMLLYAILAVFIAGLMVGRTPEYLGKKIEARE
                     VKMTMLAFLCLPLASLPFLAIACVYPTALAGLANSGPHGFSEMLYAYDSAMGNNGSAF
                     AGLTANAPWWDTTLGIAMLIGRFMFIVPMMAVAGSLAAKKLVPPSAGTFPVNSALFIA
                     LLVGVILIVTGLTYFPALALGPIVEHFSMTAGTLY"
BASE COUNT          548 a         1006 c         1080 g          520 t
ORIGIN      
        1 cagcttggtc ggatcggaat cgaggtccac catgttgccg gcctcgcgcg cggcctgcgt
       61 gccggtctgc atggccacac ccacgtcggc ctgcgccagc gccggcgcgt cgttggtgcc
      121 gtcgccgcac atggcgacga gccgcccttc ggcctgctcc ttgcggatca gcgccagctt
      181 gtcctcgggc ttggcctgcg ccaggaaatc atcgacgccg gattcggcgg cgatggccgc
      241 ggcagtcagc gggttgtcgc cggtgatcat caccgtgcgg atgcccatgc ggcgcagttc
      301 ggcgaagcgc tcgcggatgc cgcccttgac gatgtccttc aggtggatga caccgagcgc
      361 gcggttgtct ttcgccaccg ccagcggcgt gccgccggag cgcgaaatgc catcagcgat
      421 ttccttcagt tccttcaccg cgccgctcct gcccatcttc gacaggtgct cgatgatcgc
      481 ttccaccgcg cccttgcgga tgctgtggcc atcgacatcg acgccggaca tgcgcgtctg
      541 cgcggagaag ggcacgaagc gcgcgtgcag cggcgccacc tcgcgcccgc gcagcgcgta
      601 cttctccttg gccagcacca cgatgctgcg gccttcaggg gtttcgtccg acagcgaggc
      661 gagctgcgcg gcatcggcca gctccgcggt ggtcacgccg accagcggga tgaaatcgct
      721 ggcctggcga ttgcccagcg tgatggtgcc ggtcttgtcg agcagcagca cgtcgacatc
      781 gccggcggct tccaccgagc ggccggaggt ggccagcacg ttgaagcgca ccaggcggtc
      841 catgccggcg atgccgatcg ccgagagcaa tgcaccgatg gtggtcggga tcagggtgac
      901 gaacagcgcc accagcacga tcacgccgac gttgccgccg gcatacagcg cgaacgcggg
      961 aatggtggcg gtggcgaaga cgaagatcag gctcatcccc gcgagcagga tgttgagcgc
     1021 gatctcgttc ggcgtcttct ggcgcgacgc gccttcgatc agcgcgatca tgcggtcgag
     1081 gaagctgccg ccggcggtgg cggtgatgcg gaccaggatg tgatcagaca gcacgcgcgt
     1141 gccgccggtc accgcgctgc ggtcgccgcc ggcttcgcgg atcacgggtg ctgattcgcc
     1201 ggtgattgcc gactcatcga cggtagccac gcccatgatc acgtcgccgt cgcccggaat
     1261 cacttcgcca gccttgacca tggccacgtc gccgactttc agttgcgacg ccggaacact
     1321 ccgcgtggcc tgcgagtttg gcgagtcggg cgacaccagc acgtgcgcga tggtctgagt
     1381 gcgcgtcttg cgcaggctct cggcctgcgc cttgccgcgc ccttcggcaa tggcttcgga
     1441 gaaattggca aacagcacgg tgaaccacag ccacagcacc acctgcccgg agaacagcag
     1501 ctggccgcgt ccttcgagca gatcgcgcag caagatcgcg gtcgccagca gcgagaccag
     1561 cgcggtgatg aagatcaccg ggttgcggat cagcgtgcgc ggatcgagct tgacgaagga
     1621 atcgcggatg gccgggccga gcagcgcgcg gtcgaacggc gaggacgatt tgcggttgga
     1681 agtcatggca tccgactcag tagagcgtgc cggccgtcat cgaaaaatgt tcgacaatcg
     1741 gtccaagcgc gagcgcgggg aagtaggtga ggccggtgac gatgaggatc acgccgacca
     1801 gcagcgcgat gaacagcgcc gagttcaccg ggaaggtgcc ggccgacggc ggcaccagtt
     1861 ttttcgcggc caggctgccg gccaccgcca tcatcggcac gatgaacatg aagcggccga
     1921 tcagcatggc gatgccgagg gtggtgtccc accacggcgc attggcggtg agacccgcga
     1981 acgcgctgcc gttgtttccc atggccgagt cgtaggcgta aagcatctcg gagaagccgt
     2041 gcgggccgct gttggccagg ccggcgagcg cggtggggta cacgcaggcg atggccagga
     2101 acggcagcga ggccagcggc aggcacagga aggcgagcat ggtcatcttc acctcgcgcg
     2161 cctcgatctt cttgccaagg tattccggcg ttcggccgac catcagcccg gcgatgaaca
     2221 ccgcgaggat ggcgtagagc agcatgccgt acaggccact gccgacgccg ccgtagatca
     2281 cctcgccgag catgatgttg agcatcggaa tcagtccgcc gagcggcgtg tagctgtcgt
     2341 gcatggaatt caccgagcca ttcgacgcgt cggtggtggc ggtggcccac agcacggaat
     2401 cggcgatgcc gaagcgcgct tccttgcctt ccatgttgcc gccggcctgc agcgtgctgg
     2461 cgtgctggtc gatgcccatc gcatcaaacg ccgggttgcc gccggcttcc gccgcgtagc
     2521 aggccagcgc gccggccagg aacaacacgc tcatcgccga gtacagcacc cagccctgca
     2581 gcggattctt caccatgcgg ccgaaggtgt tggtcagccc cgcgctgatc accaggatgg
     2641 acagcagttc gagcaggtcg gacagcgccg tcgggttctc gtagggatgc gcgctgttgg
     2701 cgttgaagac gccgccgccg ttggtgccca gcatcttgat cgcttcctgc gaagcgacgg
     2761 ggcccagggt gatggtctgc ctggcgcctt ccaaagtcgt cgcgtcgatc tgctgggtca
     2821 gagtctgcgg cacgccgctg gcgatcagga aaatcgtgta gacgacacag atcggcagca
     2881 gcacatagag ggtgatgcgg gtcgcatccg cccagaaatt tccgatcgtc gaagtctcgc
     2941 gccgggcgaa accgcggaag aaggcgaagg cgagcgcgat gccggttgca gccgacatga
     3001 agttgtggat caccagcgcg aacatctgac tgagattcga gagaacggct tcgcccgaat
     3061 accattgcca attcgtattg gtggtgaagc tgaccgcggt gttgaacgcg ccgtcggcgc
     3121 cgatgttggc gaagtgcggc gtattcagcg gcaa
//
LOCUS       DASZZC010000685         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1271178,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000685 DASZZC010000000
VERSION     DASZZC010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271178"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..722)
                     /locus_tag="VHE37_14505"
     CDS             complement(273..722)
                     /locus_tag="VHE37_14505"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HVT36798.1"
                     /translation="MMSLIIHAALGLVTVVLFFWFNAYLYKPGWAGARVSALEGVYYA
                     FALVSVCIGWYFDIQYVFTYPNEYSWAHFIKLLFTNPAAGSGSQDLIITNTFLFPLWT
                     IIDGPRRGMKQCWIYFVMSLFTSFGFAMGLYLAALERQVRWQQAQPQ"
     gene            886..1212
                     /locus_tag="VHE37_14510"
     CDS             886..1212
                     /locus_tag="VHE37_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HVT36799.1"
                     /translation="MNADNPSRRRFFQLAAAGAACAAFAGDVLAAAAPKLSVDDPTAK
                     ALGYTEDASKIDASKEATYKKGSACASCALFQAAQAAGGYAPCGAFGGKQVNAKGWCR
                     AYAAKA"
     gene            complement(1301..>1638)
                     /locus_tag="VHE37_14515"
     CDS             complement(1301..>1638)
                     /locus_tag="VHE37_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36800.1"
                     /translation="NKINTLISNIRIAGAVGGAAGLKNINRTFSAPAQPDPPTGRSLS
                     VDRFNEGVLVRCPHCQATAERTVRWFFQNPSCAGCGHDLTLAVERALDDAGNASRWPL
                     VEPGCAAGS"
BASE COUNT          291 a          546 c          534 g          267 t
ORIGIN      
        1 gggattggcc gcatggctgc gccggcggcg gcgcggctag gcacggctcc ctggcgcttc
       61 agacactttg cccgccgtcg cacaggcggc gcagatgcat gaacgcccgc gctcggccgc
      121 tggcacctgc gcaagcaggc cggcgctgaa cgagacttcc cggcaccagc acggcgtgtc
      181 gaacgtgccg ctgcgtgcgg ccgcgcatcg gttcggcccg ccgcacagcg gacagcggtg
      241 gttcggcagc ggttgcatgg ccggctgctc actcactgcg gctgcgcctg ctgccagcga
      301 acctgccgct ccagcgccgc gagatacagg cccatggcaa aaccgaagct ggtgaacagg
      361 ctcatcacga agtagatcca gcactgcttc atcccgcgcc gaggaccatc gatgatggtc
      421 cacagcggaa acaggaaggt gttggtgatg atcagatcct gcgacccgga gccggcggcc
      481 gggttggtga acagcagctt gatgaaatgc gcccagctgt actcgttcgg atacgtgaac
      541 acgtactgga tgtcgaagta ccagccgatg cataccgaca ccagcgcgaa agcgtagtac
      601 acgccttcga gcgcggacac gcgcgcgccg gcccagcccg gcttgtacag gtaggcgttg
      661 aaccagaaga acagcaccac cgtgaccagg ccgagcgcag cgtggatgat cagcgacatc
      721 atggttgttc tctccctgtg gcggaccgtg gctccccgcc cggtcctgcg caccagcata
      781 cgcgcgcgcc ggcgcgatca cccgccggta ccgcattgac gtacgtcaac ctgcccgcgc
      841 aggcggggtt tagactggcg cacctttcac ccgcgaggtc gtgccatgaa cgctgacaat
      901 ccttcccgcc gccggttctt ccagctcgcc gctgcgggcg ccgcctgcgc cgcatttgcc
      961 ggcgatgtgc tcgcggctgc tgcgccgaaa ctctcggtgg acgaccctac ggccaaggcg
     1021 ctgggttaca ccgaggacgc gtcgaagatc gatgcatcca aggaagcgac ttacaagaaa
     1081 ggcagcgcct gcgcctcgtg cgcgctgttc caggccgcgc aggctgcggg cggttatgcg
     1141 ccgtgcggcg cgttcggcgg caagcaggtc aacgccaagg gttggtgccg cgcgtacgcg
     1201 gcgaaggcct agtcgggaac ggtccggcgc ctcggtcgcg cgaaccaggc gccgccctgc
     1261 tacgaattac gtagcaggcg agttctgcgt tgaaagcgga ctagctgccg gcggcgcagc
     1321 ccggctcgac cagcggccac cggctggcgt tgccggcatc gtcgagcgcc cgctcgaccg
     1381 caagcgtcag gtcatggccg cagccggcgc atgacggatt ctggaaaaac cagcgcaccg
     1441 tccgctctgc cgtagcctgg cagtgcggac agcgcacgag caccccttcg ttgaaccgat
     1501 ccacggacaa gcttctccca gtcggtggat caggctgggc gggagcggaa aaagttcgat
     1561 ttatgttttt gaggcccgcc gcgcctccca ccgcaccggc aattctgatg ttactaatta
     1621 atgtatttat tttatttg
//
LOCUS       DASZZC010000686         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1022641,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000686 DASZZC010000000
VERSION     DASZZC010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022641"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /gene="argG"
                     /locus_tag="VHE37_14520"
     CDS             complement(<1..787)
                     /gene="argG"
                     /locus_tag="VHE37_14520"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HVT36801.1"
                     /translation="MANILQKVPVGERVGIAFSGGLDTSAALRWMRNKGAKPYAYTAN
                     LGQPDEADYDAIPRKAMEYGAEKARLIDCRAQLVAEGIAALQAGAFHIATAGVPYFNT
                     TPLGRAVTGTMLVLAMKEDGVHIWGDGSTYKGNDIERFYRYGLLANPSLRIYKPWLDQ
                     AFIDELGGRKEMSEFMIKEGFQYKMSVEKAYSTDSNLLGATHEAKDLEHLNAGIKIVN
                     PIMGVAFWRDDVAVKAETVSVRFEQGQPVALNGQGFRDDVELLL"
     gene            complement(923..1690)
                     /locus_tag="VHE37_14525"
     CDS             complement(923..1690)
                     /locus_tag="VHE37_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HVT36802.1"
                     /translation="MQIKNSVFIVSGGASGLGLATARQLVSLGGRAVLADVNEAAGAA
                     ALAELGASARFIKTDVTREADCAAAVALAVKEFGALHGAVNCAGVGTPTKVLGKEGPH
                     PLDQFARVININLVGTFNLLRLAAAQMQNNAPNDNGERGVLINTASVAAYDGQIGQAA
                     YSASKAGVVGMTLPIARELARFGIRCMTIAPGLFETPMMASLPKEAQESLGKSVPFPP
                     RLGRPGEYALLVQQIVENVMLNGETIRLDGALRMAPK"
     gene            complement(1855..2535)
                     /locus_tag="VHE37_14530"
     CDS             complement(1855..2535)
                     /locus_tag="VHE37_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36803.1"
                     /translation="MKHPATRARFALIDLWHGLKPLALIAVLTLAIYAAVSWLDPQWP
                     AWNTQALPLWLKAWLALCIVLLLLLPRRAVAVPVPALNWRLLLAAAALPALWLALARA
                     QWLQRYLGPLMIAAAGITVIGSGIYISMDARRRRHQRGPREEEVLAEIGRRAEQSTAG
                     GMAQLRRQYPDVFARIASRFDTHEDRTAVWLMRPHPALANQRPIDVLGMPGGEEKVLQ
                     AFEAGPNA"
     gene            2617..3789
                     /gene="bcsZ"
                     /locus_tag="VHE37_14535"
     CDS             2617..3789
                     /gene="bcsZ"
                     /locus_tag="VHE37_14535"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF008305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HVT36804.1"
                     /translation="MTIIPNRLDPPMFLHQWRKFTVLAAAVLAGMVACGATSDSAWPD
                     FDTFAGRFLQNDGRIIDITFDGKSTSESQSYGMFFALVANERERFDTILKWTSANLAD
                     NQLGARLPAWNWGKKDDGSWGVRDQNSAADSDLWIAYDLFEAARLWHEPRYADTARKL
                     LGQIRAREVAELAGVGPLLLPGPEGFKLSGDRLRLVTSYYPPFMFRYLAAVDPQGPWL
                     SIWNGFLPLALKACARGVAPDIFVAAPGGMVLPDSEAAPVASYDAIRVYTWAAMSGEA
                     GAPLVKQLTPYAALIHRYGAPPEKVDPVSGRVIADQYLPLGYSGAVLPFLKAIGDEDE
                     LQRQQRRLAEAALKHRVSLGQKPNYYDEALTLFGQGWVEGRYRFDPAGRLQPRWVQ"
BASE COUNT          638 a         1290 c         1323 g          669 t
ORIGIN      
        1 ccagcagcag ttccacgtcg tcgcggaagc cctggccgtt gagtgcaacc ggctggccct
       61 gctcgaagcg cacgctcacc gtctcggcct tcactgcgac atcgtcgcgc cagaaggcga
      121 cgcccatgat cgggttgacg atcttgatgc cggcgttgag atgctcgagg tccttggctt
      181 cgtgcgttgc gcccagcagg ttggagtcgg tggaataggc cttctccacg ctcatcttgt
      241 actggaagcc ttccttgatc atgaactccg acatttcctt gcggccgccg agttcgtcga
      301 tgaaggcctg gtccagccag ggcttgtaga tgcgcaggct cggattggcc agcaggccgt
      361 agcggtagaa gcgctcgatg tcgttgccct tgtaggtgct gccgtcgccc cagatgtgga
      421 cgccgtcttc cttcatcgcc agcaccagca tcgtgcccgt cactgcgcgg cccagcggcg
      481 tggtattgaa gtaaggcacg ccggcggttg cgatgtggaa cgcgccggcc tgcagtgcgg
      541 cgatgccctc ggcaaccagt tgcgcgcggc aatcgatcag cctggctttt tccgcaccgt
      601 actccatcgc cttgcgcggg atggcgtcgt agtcggcctc gtcgggctgg ccgaggttgg
      661 cggtgtaggc atagggcttg gcgcccttgt tgcgcatcca gcgcagcgcc gcgctggtgt
      721 ccagaccgcc tgaaaaggcg atgccgacgc gctcgcccac cggaactttc tgcaggatgt
      781 tggccatagg ggcgcgatga taaatgttga cggactggta ctgaagctgc atccccgctt
      841 tcgcggggat gacatcaggc tgcgtgttgc atgaaaaccg gatccccgcc tgcgggggga
      901 tgacatcagg ctgcgcctga tgtcatttgg gcgccatgcg cagcgcgccg tcgaggcgga
      961 tggtttcgcc gttgagcatc acgttctcca cgatctgctg caccagcagg gcatattcgc
     1021 ccgggcggcc caggcgcggc gggaacggca cgctcttgcc cagcgattcc tgcgcttcct
     1081 tgggcagcga ggccatcatc ggcgtctcga acaggcccgg cgcaatggtc atgcagcgga
     1141 tgccgaagcg cgccagctcg cgcgcgatgg gtagcgtcat gccgaccacg ccggccttcg
     1201 acgccgagta cgcggcctgg ccgatctgcc cgtcataggc ggccacgctg gcggtgttga
     1261 tcagcacgcc gcgctcgcca ttgtcgttgg gcgcattgtt ctgcatctgc gccgcggcca
     1321 ggcgcagcag gttgaaggtg ccgaccaggt tgatgttgat tacgcgcgca aactgatcga
     1381 gcggatgcgg gccttccttg cccagcacct tggtcggcgt gccgacgccg gcgcagttga
     1441 cggcgccgtg cagcgcgccg aattccttca ccgccagcgc caccgcggcg gcgcagtcgg
     1501 cttcgcgcgt gacatcggtc ttgatgaagc gcgcggaggc gcccagctcg gcgagcgccg
     1561 cggcgccggc ggcttcgttc acgtcggcca gcacggcgcg gccgccgagc gaaaccagct
     1621 ggcgcgcggt ggccaggccg agtccggagg cgccgccgga gacgatgaac acgctgttct
     1681 tgatctgcat ggaatccttc gttgagctgg agaggaaaaa atgccgtcac cgcaccccgg
     1741 ttttcgatgt tataaaaaat cgtattaaat tataataata caataattat taacatcacc
     1801 aggggccgtg cggtcagcct attttgaggc catgcgggcc gccgcgtcag cctgtcaggc
     1861 gttggggccg gcttcgaagg cctgcagcac tttctcctcg ccgccgggca tgcccagcac
     1921 gtcgatcggc cgctggttgg ccagcgccgg gtgcggccgc atcagccaga ctgcggtccg
     1981 gtcttcgtgc gtgtcgaagc ggctggcgat acgcgcgaag acgtccgggt actggcgccg
     2041 cagctgcgcc atgccgccgg cggtggattg ctccgcgcgg cggccgattt ccgccagcac
     2101 ttcttcctcg cgcggtccgc gctggtgccg gcgccggcgc gcatccatgg aaatgtagat
     2161 gccgctgccg atgacggtga tccccgcggc ggcgatcatc agcgggccta gatagcgctg
     2221 cagccattgc gcacgtgcca gcgccagcca cagcgccggc agcgccgcag ccgccagcag
     2281 caggcgccag ttgagtgcgg gtaccggcac ggcaacggcc ctgcgcggca gcagcaacaa
     2341 caaaacgatg cagagcgcga gccaggcttt cagccagagt ggcagggcct gcgtattcca
     2401 cgcaggccat tgcggatcaa gccagctgac cgccgcgtag atggcgagcg tgagtaccgc
     2461 gatcagggca agcggcttga gcccgtgcca caggtcgatc agggcaaagc gcgcacgcgt
     2521 ggctggatgc ttcatgcaaa aggcgtcctt tcctttgcgc cgtatcgtat acccgccgtg
     2581 gcagcgggag aaccgcggca tgcgcggggc agtcgaatga cgatcatccc caacagactg
     2641 gacccgccga tgttcctgca ccagtggcga aaattcaccg tgctcgccgc cgccgtgctt
     2701 gcgggcatgg tcgcctgcgg cgccacgtcc gacagtgcct ggccggattt cgacacgttc
     2761 gccgggcgat tcctgcagaa cgacggccgc atcatcgaca tcaccttcga cggcaagagc
     2821 acctcggaaa gccagtccta cggcatgttc ttcgcgctgg tggccaatga gcgcgagcgc
     2881 ttcgacacca tcctcaagtg gacctcggcc aacctggccg acaaccagct cggcgcgcgc
     2941 ctgccggcat ggaactgggg caagaaggac gacggcagct ggggcgtgcg cgaccagaac
     3001 tcggcagccg acagcgacct gtggatcgcc tacgacctgt tcgaggccgc gcgcctgtgg
     3061 cacgagccac gctatgccga caccgcgcgc aagctgctcg ggcagatccg agcgcgcgaa
     3121 gtggcggagc tggccggcgt cggcccgctg ctgctgccgg ggccggaagg cttcaagctg
     3181 tcgggcgatc ggctgcggct ggtcaccagc tactacccgc cgttcatgtt ccgctacctg
     3241 gccgcggtcg atccgcaggg accgtggctg tccatctgga acggcttcct gccgctggcg
     3301 ctgaaggcct gcgcacgtgg cgttgcgccc gacatcttcg ttgccgcgcc gggcggcatg
     3361 gtgctgcccg acagcgaagc ggcaccggtg gcgagctacg atgccatccg cgtctacacc
     3421 tgggcggcga tgtccggcga agccggcgcg ccgctggtga agcagctcac gccgtacgcc
     3481 gcgctgatcc accgctacgg cgcaccgccg gagaaggtcg acccggtcag cggccgcgtg
     3541 atcgccgatc aatacctgcc gctgggttat tcaggcgcgg tgctgccctt cctcaaggcg
     3601 attggcgacg aggatgaatt gcagcggcag cagcggcggc tggccgaggc ggcgctcaag
     3661 catcgcgtca gcctcggcca gaagcccaac tactacgacg aggcgctcac gctgtttggg
     3721 cagggttggg tggaaggacg ctaccggttt gacccagcgg ggcgtctgca gccgcgctgg
     3781 gtgcagtgaa gctgcgcacc acctgtatcg aggcggccga cgacggcgcg cgcacgccga
     3841 aatgcccttc gcgcagcagg cgccacaggc cgggaaccgc attcatgtaa cgccgcgcca
     3901 gccggcgcgg attctgcagc
//
LOCUS       DASZZC010000687         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1151127,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000687 DASZZC010000000
VERSION     DASZZC010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151127"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VHE37_14540"
     CDS             <1..527
                     /locus_tag="VHE37_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT36805.1"
                     /translation="YQQRVGLAQAIIHRPPVIVLDEPTVGLDPIQIREIRNLIVELGK
                     SHSVILSSHILPEIQAVCGRVMIINRGRVVYNEAVAAARGGERVIAAFRNTPDSGALG
                     AIAGVRSVSALEPGRWSFGTDNGADPREAIVQAAVKNDWGLLELRAQERTLEEIFVDL
                     TSGDEGVRNAGRAA"
     gene            524..1264
                     /locus_tag="VHE37_14545"
     CDS             524..1264
                     /locus_tag="VHE37_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT36806.1"
                     /translation="MSNILNIAGCELRRIFLSPLAWAVLAIVQFIMAFVFVNLLVEYA
                     NADSDQTMGVSDYIGGSVYGFATIILLMVMPLMTMRLFSEERKSGSITLLFSSPVSLT
                     EIVLGKFLGLMGFVLAMLLLMALLPASLQWSTHLDWGRIAAGLFGLFLLMMSFGAAGM
                     FVSSLTREPTIAAVAGFGLLLIVWLLSILAYNDSIPLHDLINYLSLIGHYESMRRGMF
                     DSADALYYVLFSVLFLWLTVQRLDMERN"
     gene            1280..2623
                     /locus_tag="VHE37_14550"
     CDS             1280..2623
                     /locus_tag="VHE37_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HVT36807.1"
                     /translation="MKKNSQLAQQLLSALLFFAVIVMLGWLSVRYKTQLDWTAGKRNT
                     LTEASVKLLDSMKDPITLHAFVPSGSDDRRGLEQDLARYTRVKKDITLDFIDPTTHPQ
                     DVQKYNIQQVGEVVLEYQGRRETLHATTEQAISTALQRLSYGGEQWVVFLEGHGERSA
                     NDSQAQEAAGKFAQALRDKGLKVNSLSLVKQPKVPDNASVLVIASPTAQPIAGEVELI
                     KKYLDDGGDLLWLADPESPAGMEPLAKALGISWQDGYVIDPVAQQLGLPAGFYVPGDY
                     PPNPVTQGMEHVTLFPLARSLTYDKNAGWQAQEMLKSVDESWLETGDISKGEVKFDGK
                     DIKGPLTVGLTLTRQHKGADGKEHAQRVALIGDVDFLSNAFIAQQGNQQLAINLVQWL
                     ASRDAQLNIDIPKAPDVSLYLPGWGLKLIVLGLCIVLPLSLLGYGVGRWIVRRRK"
BASE COUNT          457 a          903 c          881 g          438 t
ORIGIN      
        1 gctaccagca gcgcgtgggc ctggcgcagg ccatcatcca ccggccgccg gtgatcgtgc
       61 tcgacgagcc caccgtgggc ctggacccga tccagatccg cgagatccgc aatctcatcg
      121 tcgagctggg caagagccac agcgtgatcc tgtcctcgca catcctgccg gagatccagg
      181 cggtgtgcgg gcgcgtgatg atcatcaacc gcggccgcgt ggtgtacaac gaggcggtgg
      241 ctgccgcgcg cggcggcgag cgcgtgatcg ccgccttccg caacacgccg gacagcggcg
      301 cgctgggtgc catcgccggc gtgcgcagcg tgtccgcgct ggagccggga cgctggagct
      361 tcggcaccga caacggcgcc gatccgcgcg aggccatcgt gcaggccgcg gtgaagaacg
      421 actggggcct gctcgaactg cgcgcgcagg agcgcacgct ggaggaaatc ttcgtcgacc
      481 tcaccagcgg cgacgaaggc gtgcgcaacg ccgggagagc cgcatgagca acatcctcaa
      541 catcgccggc tgcgagctgc gccgcatctt cctgtcgccg ctggcctggg cggtgctggc
      601 catcgtgcag ttcatcatgg ccttcgtctt cgtcaatctg ttggtcgagt acgccaacgc
      661 cgacagcgac cagaccatgg gcgtgtccga ttacatcggc ggctcggtct acggcttcgc
      721 caccatcatt ctcctgatgg tgatgccgct gatgaccatg cggctgttct cggaagagcg
      781 caagagcggc agcatcacgc tgctgttctc ctcgccggtg tcgctcaccg agatcgtgct
      841 gggcaagttc ctggggctga tgggcttcgt gctggccatg ctgctgctga tggcgttgct
      901 gccggcctcg ctgcagtgga gcacacatct ggactggggc cgcatcgccg ccgggctgtt
      961 cggcctgttc ctgctgatga tgtccttcgg cgccgccggc atgttcgtgt cgtcgctgac
     1021 gcgcgagccg accatcgccg cggtggccgg cttcggcctg ctgctgatcg tgtggctgct
     1081 gtcgatcctt gcctacaacg acagcatccc gctgcacgac ctgatcaact acctgtcgct
     1141 gatcggccac tacgagagca tgcgccgcgg catgttcgat tccgccgacg cgctgtatta
     1201 cgtgctgttc tcggtgctgt tcctgtggct caccgtgcag cggctggaca tggaacgcaa
     1261 ttaataaaag aacgacgcca tgaaaaagaa ttcgcaactc gcccagcagc tgctcagcgc
     1321 gctgctgttc ttcgccgtca tcgtcatgct cggctggctg tcggtgcgct acaagacgca
     1381 gctcgactgg accgccggca agcgcaacac gctgaccgag gccagcgtga agctgctcga
     1441 ctcgatgaag gacccgatca cgctgcacgc cttcgtcccc tcgggcagcg acgaccgccg
     1501 cgggcttgag caggacctgg cgcgctacac ccgggtgaag aaggacatca cgctcgactt
     1561 catcgacccc accacgcatc cgcaggacgt gcagaagtac aacatccagc aggtcggcga
     1621 agtggtgctg gaataccagg gccgccgcga aaccctgcac gccaccaccg agcaggccat
     1681 cagcaccgcg ctgcagcgcc tgagttacgg cggcgagcag tgggtggtgt tcctcgaagg
     1741 ccacggcgag cgctcggcca acgacagcca ggcgcaggaa gccgccggca agttcgcgca
     1801 ggcgctgcgc gacaaggggc tcaaggtcaa ttcgctgtcg ctggtgaagc agcccaaggt
     1861 gccggacaac gccagcgtgc tggtcatcgc ctcgcccacc gcgcagccca tcgccggcga
     1921 agtggagctg atcaagaagt acctcgacga cggcggcgac ctgctgtggc tggccgatcc
     1981 agaatcgccg gccggcatgg agccgctggc caaggcgctg ggcatcagct ggcaggacgg
     2041 ctacgtcatc gacccggtgg cgcagcagct cgggctgccc gccggcttct acgtgcccgg
     2101 cgactatccg cccaacccgg tcacgcaggg catggagcac gtcaccctgt tcccgctggc
     2161 gcgctcgctc acctacgaca agaacgccgg ctggcaggcg caggaaatgc tcaagtcggt
     2221 cgacgaatcg tggctggaga ccggcgacat ttccaagggc gaggtgaagt ttgacggcaa
     2281 ggacatcaag ggtccgctca ccgtcgggct cacgctcacg cgccagcaca agggcgccga
     2341 cggcaaggag cacgcgcagc gcgtggcgct gatcggcgat gtcgacttcc tgagcaacgc
     2401 cttcatcgcg cagcagggca accagcagct ggcgatcaac ctggtgcagt ggctggcctc
     2461 gcgcgacgcg cagctgaaca tcgacatccc caaggcgccg gacgtgtcgc tgtacctgcc
     2521 gggctggggc ctcaagctga tcgtgctcgg cctgtgcatc gtgctgccgc tgtcgctgct
     2581 gggctacggc gtcggccgct ggatcgtgcg ccggcgcaag tgaggttgcg cacatgaacc
     2641 gcacgcaact caacctcgcc ctgctggcgc tggccgccg
//
LOCUS       DASZZC010000688         7005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1151171,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000688 DASZZC010000000
VERSION     DASZZC010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151171"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VHE37_14555"
     CDS             <1..1125
                     /locus_tag="VHE37_14555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT36808.1"
                     /translation="HRTSGAARASLEDVSDDAQRTYFCLDGMRGVAALAVLTAHAAML
                     FQHTLRQFYLAVDFFFVLSGFVLAYAYSARLAAGMPAREFIIARLVRIYPLYLIATLI
                     GLAHAALSPNSAEAAPLPVLLGQLALALFFVPAPALTPGHWLYPLVDPGWSLLFELLG
                     NIVFGLLWLRLHRSRPGLALLIGCSAIALLYCGFSFGTFDLGATSGDAWAAVPRVLYS
                     FFAGVCLYQWHRRHPAPLRIDSWLLLAALTVALALHFPSRTLDLAYQYAMIVLGFPLL
                     VYLGASSTPSARAAPLFHLLGVTSYAVYVLHVPLFFCLRDSSAHFGWNLAAYAPWSGL
                     AFIAALLAFCVAIDRWYDVPLRRWLNQLRRTRPLAVRAAS"
     gene            complement(1164..2987)
                     /locus_tag="VHE37_14560"
     CDS             complement(1164..2987)
                     /locus_tag="VHE37_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HVT36809.1"
                     /translation="MNRSRLFVLGSACCVFLLAGCGGAAAASTNPVKATSSATTTTTA
                     ASTTSSAGYHASNGRLYDAAGNELVVRGISNFGFNGTTLQPQYLWQMGWKEQIDQIKA
                     LGFNAVRLPFVPDTLYNTTPVDQLSYVDGGKNPDLIGKTPLQALDLWMAYADSVGLYV
                     MLDFHSVSMQRQYPTWFVSDANDFGLIYNHSAYSEQHWIRDLTFVARRYAKLAHFFAI
                     DLYNEPEGAIRWSSGDPNVTNPAYFWKAASEMAGSAVLSANPNLLIFVQGINGDFDNI
                     ENSSIPMNWGEDFQPLSYQPSTIPADKLVLTPHTYGPDVFMKSTFSAPSYPANLAADW
                     ETLFGKQYPAHPVVIGEWGGRYGDGPSGQKDVQWQNALVAYLLNKGIHSSFYWCYTPN
                     SGDSGGILDDNLQVRQDKMALLHTLWGDSTKSDPTPTPTPTPTPTTSNPLPLITSFSP
                     AGGPAGTVVRVVGKRFATATHATCGNGVDLPLQVMGRTAAKVTIAAGCTSGPLTIATD
                     RKSGSSAGSFEVTASNPQQAISSFSPSSGAAGSVITLTGSGFLGSTQAWVGNAHNATL
                     TVISDTRARITVPAGATSGAVAILNAAHAAFTPHAFTMNGN"
     gene            3242..3940
                     /locus_tag="VHE37_14565"
     CDS             3242..3940
                     /locus_tag="VHE37_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HVT36810.1"
                     /translation="MADVQAGERAAQPRACPLLAELFCAGVATALLEGSAQREAQWPL
                     LGDESLAVEQAGDRRRADFAAGRACARAALAQLGVAPVAIPVNDDRSPRWPDGYTGSI
                     THTCSCAAAVAAPLRSVRALGLDAENCVDFDGTLVADVCTLRERAWLDTLAPAQRNAA
                     ATLLFSAKEAFYKCQYALTRRWLEFHEVTLEANHGSLQLRLAPADLLRLPWRGRYAQH
                     GDCVYTGFSLPTSG"
     gene            complement(4061..4903)
                     /locus_tag="VHE37_14570"
     CDS             complement(4061..4903)
                     /locus_tag="VHE37_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36811.1"
                     /translation="MKETVLTFGNQLMGVLTQPERGDLQRPVVLIPNTGIEHRVGPNR
                     LHVRIARALAEAGYASARIDLSGLGDSPAALDGNSTDDLRAAMDELSRRGAGRRFVAV
                     GLCSGSHDAHRLALADERVVGAAFLDGYAYRTPRFFATYLWQRASDPARWAAYVRKLA
                     HRAQAGDDLDVQRVDYYRQPPAAQFRSEIAALMERGLALCFIYTGQMQYIYNYACQLT
                     DRFPELRGYERFELHHMAHADHTFSTVAMKAQLAERLLTWLRRTGEIESARAAPALTV
                     VQAA"
     gene            complement(4900..>7005)
                     /locus_tag="VHE37_14575"
     CDS             complement(4900..>7005)
                     /locus_tag="VHE37_14575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HVT36812.1"
                     /translation="VIFTSGSTGRPKGAVLSHRGVVNRLLWMQDAYDIGPGDRVLQKT
                     PYSFDVSVWEFFWPLLTGATLVMAQPDGHKSPAYVAAAIEGKGITVCHFVPSLLGVFL
                     TQPELPACATLRYVFCSGEALPYQTTSQFRERLPHVQLHNLYGPTEASVDVSHWRCEP
                     DEARRLVPIGRPVANTQLYVLDADLEPLPVGVPGQLYLGGVQLATGYLKRPQLTAERF
                     ITHPRFGRLYVTGDAARWLPDGAVDFLGRLDGQVKLRGQRIELGEIEARLDALPQVAE
                     SACAVRARTAGDERLVAWVVPAAGACIDAGELADALRRDLPEFMIPQHIVAIGALPRL
                     TSGKIERKALPELAELPVPRRAALPPATPAEKAVAAVWQQLLGTAAVNRDDRFFDLGG
                     HSLLAVQAVTLLKQRFGVKPSLRSAMMATLAGLAQEVEGQSPAAARPVQDSKPTALSR
                     QEAFYFGAAGERLFGVITRPALPRKAQAILICQSWGVEYMRSHRALQLLAQRLAESGF
                     CVMRFDYYGTGDSAGESRGAGVQDWVRNIRAAARELRTRTGLHRLALLGVRLGALLAV
                     HAREEGLDADQLLLWDPPPDGAKWLAELTRLCQLSHDNWNRQRPRSTKLPPPPADQIF
                     GMTVSQEWLDGIAQLAPPLAGNGVRIVQSRDEAPAAAPVVQALPDDSCWAQLGWITRP
                     WNPRASSEQVANLLERTLP"
BASE COUNT          988 a         2408 c         2423 g         1186 t
ORIGIN      
        1 caccgaactt cgggcgccgc ccgcgcgtcg ttagaggatg tgagcgacga cgcgcagcgg
       61 acctatttct gcctcgacgg catgcgcggc gtggctgcgc tggcggtgtt gacggcgcat
      121 gccgccatgc tgttccagca cacgctgcgc cagttctacc tggcggtgga tttcttcttc
      181 gtgctgtcgg gcttcgtgct ggcctacgcc tactccgcgc gcctggccgc cggcatgccg
      241 gcgcgcgaat tcatcatcgc gcgcctggtc cgcatctacc cgctgtacct gatcgccacg
      301 ctgatcgggc tcgcgcacgc cgcgctgtcg cccaactcgg cggaggccgc tccgctgccg
      361 gtgctgctcg gccagctggc gctggcgctg ttcttcgtgc cggcgccggc gctgacgccg
      421 ggacactggc tctacccgct ggtcgacccg ggctggtcgc tgctgttcga gctgctcggc
      481 aacatcgtgt tcggcctgct gtggctgcgc ctgcatcgca gccggcccgg gctggcgctg
      541 ctcatcggct gctcggcgat tgcgttgctg tactgcggtt tttccttcgg cacgtttgac
      601 ctcggcgcga cttccggcga cgcctgggct gcggtgccgc gcgtgctcta ctcgttcttc
      661 gccggcgtgt gcctgtacca atggcaccgg cgccatcctg cgccgctgcg gatcgactcg
      721 tggctgctgc tcgcggcgct caccgttgcg cttgcgctgc attttccctc gcgcacgctg
      781 gatctggcct atcaatacgc gatgatcgtt ctgggcttcc cgctgctggt ctatctcggc
      841 gcctcatcga cgccctcggc gcgcgcggcg ccgctgttcc acctgctcgg cgtcacttcc
      901 tacgcggtct acgtgctgca cgtgccgctg ttcttctgcc tgcgcgacag ctctgcgcat
      961 ttcggctgga acctcgccgc gtacgcgccg tggagcgggc tggccttcat tgcggcgctg
     1021 ctcgctttct gcgtggccat cgaccgctgg tacgacgtgc cgctgcgccg ctggctcaac
     1081 cagcttcgca ggacgcggcc cctagccgtc cgggccgcgt cctgacccgc ctcatcccac
     1141 caccgtacac ccctacaaca agatcagttg ccgttcatgg tgaacgcgtg cggcgtgaag
     1201 gccgcgtgcg ccgcgttgag gatcgccacc gcgccgctgg tggcgccggc cggcaccgtg
     1261 atccgggcgc gcgtgtcgct gatcaccgtg agcgtggcgt tgtgcgcgtt gccgacccag
     1321 gcctgcgtcg agccgaggaa gccgctgccg gtgagtgtga tcacgctgcc cgccgcgccc
     1381 gaagacggcg agaaggaact gatcgcctgc tgcggattcg acgcggtgac ctcgaacgag
     1441 cccgcgctgc tgccgctctt gcgatcggtg gcgatggtca gcggtccgct ggtgcaaccc
     1501 gcggcaatcg tcaccttggc cgccgtgcgg cccatcacct gcagcggcag atccacgccg
     1561 ttgccgcagg tggcatgcgt ggcggtggcg aagcgcttgc cgaccacgcg caccaccgtg
     1621 cccgcgggcc cgccggcggg cgagaagctg gtgatcagcg gcagcgggtt gctggtcgtg
     1681 ggagtgggag tgggagtcgg cgtcggcgtg ggatcggact tggtgctgtc tccccacagt
     1741 gtgtggagca gcgccatctt gtcctggcgc acctgcaggt tgtcgtcgag gatgccgccg
     1801 gagtcgccgc tgttgggcgt gtagcaccag tagaagctgc tgtggatgcc cttgttcagc
     1861 aggtacgcga ccagcgcgtt ctgccactgc acatctttct gcccgctggg gccatcgccg
     1921 tagcggccgc cccactcgcc gatcaccacc gggtgcgccg ggtactgctt gccgaacagc
     1981 gtctcccagt ccgccgcgag attggccgga tagctgggcg cgctgaaggt gcttttcatg
     2041 aacacgtccg ggccgtaggt gtgcggcgtc agcaccagct tgtcggcggg aatggtggac
     2101 ggctggtacg acagcggctg gaaatcctcg ccccagttca tcgggatgga ggaattctcg
     2161 atgttgtcga aatcgccgtt gatgccctgc acgaagatca gcaggttcgg gttggccgac
     2221 agcaccgcgc tgccggccat ctccgacgcc gccttccaga agtacgccgg gttggtcacg
     2281 ttagggtcgc cgctgctcca gcggatggcg ccctctggct cgttgtagag gtcgatggcg
     2341 aagaagtgcg cgagcttggc gtagcgcctg gccacgaagg tcaggtcgcg gatccagtgc
     2401 tgctcgctgt atgccgagtg gttgtagatc aggccgaagt cattcgcgtc gctgacgaac
     2461 caggtcgggt actggcgctg catcgacacg ctgtggaaat ccagcatcac gtacaggccg
     2521 acgctgtcgg catacgccat ccacaggtcc agcgcctgca gcggcgtctt gccgatcagg
     2581 tcgggatttt ttccgccgtc gacgtagctc agctggtcga ccggcgtggt gttgtacagc
     2641 gtgtccggca cgaacggcag gcgcaccgcg ttgaagccca gcgccttgat ctggtcgatc
     2701 tgctccttcc agcccatctg ccacaggtac tgcggctgca gcgtggtgcc gttgaagccg
     2761 aagttgctga tgccgcgcac gaccagctcg ttgccggcgg cgtcgtacag cctgccgttg
     2821 ctggcgtggt agccggcaga ggaagtggtg gaagcagcag tggtcgttgt cgtggcgctc
     2881 gacgtcgcct tgaccgggtt ggtggaagcg gcagcggcgc cgccgcaacc tgccaggaga
     2941 aacacgcagc acgcggagcc gaggacaaac agcctggaac ggttcaagga caacccctat
     3001 gaactgggca gtcctttgcc ggtgagacga cggtcgatcc gtgaccgtcg cgcgcgaatc
     3061 attcgcgtct tgccgctcct tgaaccggac ctccgacacg tccttgtgcc ccggcgcgtg
     3121 ctttgtacgc cggctgttgc agagccggac tacgcctaag tgattttgcc catcggcaaa
     3181 tcgaacccgc ccgctgtgcg cacggtcgca tccggatgat gtgcaccccg ggcaacacgt
     3241 gatggccgac gtacaggccg gcgaacgcgc cgcgcagccg cgcgcctgcc cgctgctggc
     3301 cgaattgttt tgcgcgggtg tcgccaccgc gctgctggaa ggcagcgcgc agcgcgaggc
     3361 gcagtggccg ctgctcggcg acgaatcact ggccgtcgag caggccggcg accgacgccg
     3421 cgccgatttc gccgccggcc gcgcctgcgc gcgcgcagca ctggcccagc tcggcgtggc
     3481 gccggtcgcc atcccggtca acgacgatcg cagcccgcgc tggcccgatg gctacaccgg
     3541 cagtatcacg cacacctgca gctgcgcggc tgcggtagcg gcgccgttgc gatcagtgcg
     3601 cgcgctgggg ctcgacgcgg aaaactgcgt ggacttcgac ggcacgctgg tcgccgacgt
     3661 gtgcacgttg cgcgaacgcg cgtggctgga cacgctggcg cccgcgcagc gcaacgcggc
     3721 cgccacgctg ctgttcagcg ccaaggaggc gttctacaaa tgccagtacg cgctcacgcg
     3781 gcgctggctg gaattccacg aggtgacgct ggaggcgaac cacggctcgc tgcagttgcg
     3841 cctagcgccg gcggacctgc tgcgcctgcc ctggcgcggc cgttacgcgc agcacggcga
     3901 ttgcgtctat accggcttca gtcttccaac gtctggctga tggcgttttt gggctccacc
     3961 gcatcggcac ttttgatgtt actaattata ttattattta cattttagat aattatttat
     4021 aacacgcaaa cccggggtgc ggtgaaggca tttcgcgcgt ctacgcggcc tgcaccacgg
     4081 tcagcgccgg cgccgcgcgc gccgactcga tctcgcccgt gcgccgcagc caggtgagca
     4141 ggcgctccgc cagctgcgcc ttcatcgcca ccgtggagaa ggtgtgatcg gcatgggcca
     4201 tgtggtgcag ctcgaagcgc tcgtagccgc gcagttccgg gaagcggtcg gtgagctggc
     4261 aggcgtagtt gtagatgtat tgcatctgcc cggtgtagat gaagcacagt gccagtccgc
     4321 gctccatcag cgcggcgatc tcgctgcgga actgcgcggc gggcggctgg cggtagtagt
     4381 cgacgcgctg cacgtcgagg tcatcgcccg cctgcgcacg gtgcgcgagc ttgcgcacgt
     4441 aggcggccca gcgcgccgga tcgctcgcgc gctgccacag gtaggtggcg aagaagcgcg
     4501 gcgtgcgata ggcgtagccg tcgaggaacg cggcgccgac cacgcgctcg tcggccagcg
     4561 ccaggcggtg cgcatcgtgc gagcccgagc acaggccgac ggcgacgaag cggcggccgg
     4621 cgccgcgccg cgacagctcg tccatcgccg cgcgcaggtc gtcggtggaa ttgccgtcga
     4681 gcgcggccgg cgaatcgccc aggcccgaca ggtcgatgcg cgcgctggcg tagccggcct
     4741 ccgccagtgc gcgcgcgatg cgcacgtgca gccggttggg ccccacgcgg tgctcgatgc
     4801 ctgtattggg gatcagcacc accgggcgct gcaggtcgcc gcgctcgggc tgcgtcagca
     4861 cgcccatcag ttgattgccg aaggtcagca cggtttcttt catggcaagg tccgttcgag
     4921 caggttggcc acttgttccg acgaggcgcg cggattccac ggccgcgtga tccagcccag
     4981 ctgcgcccag cagctgtcgt cgggcagggc ctgcaccacc ggcgcggcgg ccggcgcctc
     5041 gtcgcgcgac tgcacgatgc gcacgccgtt gccggcgagc ggcggcgcca gttgcgcgat
     5101 gccgtcgagc cactcctggc tcaccgtcat gccgaagatc tggtccgccg gcggcggcgg
     5161 cagcttggtg ctgcgcgggc gctggcggtt ccagttgtcg tgcgacaact ggcacagccg
     5221 ggtgagttcg gccagccact tcgcgccatc cggcggcgga tcccacaaca gcagctggtc
     5281 ggcgtcgagt ccttcctcgc gcgcgtgcac cgccagcagc gcgcccaggc gcacgcccag
     5341 cagcgccagc cggtgcaggc cggtgcgcgt gcgcagctcg cgcgcggccg cgcggatgtt
     5401 gcgcacccag tcctgcacgc cggcgccgcg tgattcgccg gcggaatcgc cggtgccgta
     5461 gtaatcgaag cgcatcacgc agaagccgct ctcagccagc cgctgcgcca gcagctgaag
     5521 cgcacgatgg ctgcgcatgt attccacgcc ccagctctgg cagatgagga tggcctgcgc
     5581 cttgcgcggc agcgccggcc gcgtgatcac gccgaacagc cgctcgccgg ccgcgccgaa
     5641 gtagaacgct tcctggcgcg acagcgcggt tggcttgctg tcctgcaccg ggcgcgccgc
     5701 ggccggcgac tggccttcga cttcctgcgc cagtccggcc agcgtggcca tcatcgccga
     5761 gcgcagcgag ggcttcacgc cgaagcgctg cttgagcagc gtcaccgcct gcaccgccag
     5821 cagtgagtgc ccgccgagat cgaagaagcg gtcgtcgcgg ttgacggcgg ccgtgccgag
     5881 cagctgctgc cacaccgcag ccacggcctt ctccgccggc gtcgccggcg gcagcgcggc
     5941 acggcgcggc acgggcagct ccgccagttc gggcagcgcc ttgcgctcga tcttgccgct
     6001 ggtgagccgc ggcagcgcgc cgatcgccac gatgtgctgc gggatcatga actccggcag
     6061 gtcgcgccgc agcgcatcgg cgagctcgcc ggcgtcgatg cacgcgccgg cggcgggcac
     6121 cacccaggcc accagccgtt cgtcacccgc ggtgcgcgca cgcaccgcgc aggcggactc
     6181 cgcgacctgg ggcagcgcgt cgagccgcgc ctcgatttca cccagctcga tgcgctggcc
     6241 gcgcaatttc acctggccgt cgaggcggcc gagaaaatcc accgcgccat ccggcagcca
     6301 gcgcgcggca tcaccggtga cgtacaggcg gccgaagcgc ggatgcgtga tgaagcgctc
     6361 cgcggtgagt tgcgggcgct tgagataacc ggtcgccagt tgcacgccgc cgaggtacag
     6421 ctggccgggc acgccgaccg gcagcggctc gaggtccgcg tcgagcacat acagctgcgt
     6481 gttggccacc ggccggccga tcggcaccag ccggcgcgcc tcgtcgggct cgcagcgcca
     6541 gtggctgaca tcgacgctgg cctcggtcgg tccgtacagg ttgtgcagct gcacgtgcgg
     6601 caggcgctcg cggaactggc tggtggtctg gtacggcagc gcttcgccgg aacagaacac
     6661 atagcgcagc gtggcgcagg ccggcagttc gggctgcgtc aggaacacac cgagcagcga
     6721 cggcacgaag tggcacacgg tgatgccctt gccctcgatg gccgcggcca cgtaggccgg
     6781 gctcttgtgg ccgtcgggct gcgccatcac cagcgtggcg ccggtgagca gcggccagaa
     6841 gaattcccac acggacacat cgaacgaata cggcgtcttc tgcagcacgc ggtcgccggg
     6901 gccgatgtcg taggcatcct gcatccacag caggcggttg acgacgccgc gatgactgag
     6961 caccgcaccc ttgggccgtc ctgtggaacc ggaggtgaaa atcac
//
LOCUS       DASZZC010000689         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1151240,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000689 DASZZC010000000
VERSION     DASZZC010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151240"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="VHE37_14580"
     CDS             <1..1678
                     /locus_tag="VHE37_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT36813.1"
                     /translation="VLRGPYSALYGNSSAGVVQVFSAGGNPPGFYSAFSGGSYGGRRA
                     AYAARGVDGALEYGLSADYFGTDGYRGHSAAQRWIGNGQLRYDFGSGGTLEVLGSSID
                     LPEAQDPLGLTATQLATDRTQATLPAVQFNTRKRVHQYQGGLVWEKKFSDALSLRVMG
                     YGGHRAIGQVLSTPVAAQTGSPLNSGGVVDLGSVYDGGDARLQYQLGALQLTGGFSYD
                     RQDQHRRGYENFSGAQMGVQGKLRRDEQDDVYNVDEYLQADWRFAQRWSLLAGVRHSE
                     VLFASNDAYIVAGNPDDGGSRNYAATTPVAGLMFRASDALHLYASYGAGFETPTFTEM
                     AYRPDGSAGLNLGLDEARSNNFEIGAKHRADGRSIEAALFEADARNELAVFSNSNGRT
                     TYHNIDRARRRGAELSASQDFSSHWNVSLAYTWVQAQFETAFANCATPASCTINGGTP
                     IPGVPRNDLYTELRWQVASGWHAGINAQALSRVYADDAGTQHAAGYALLGADAGWSAP
                     RHDARLSLYLRGENLTGARYVGSVIVNEANGRYFEPAPGRTFFAGASLRY"
     gene            1721..3031
                     /locus_tag="VHE37_14585"
     CDS             1721..3031
                     /locus_tag="VHE37_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HVT36814.1"
                     /translation="MNRAILLMAALLAAGVGAADDEPSSATPGYGYNPPLPAPAHSLI
                     PTVNIAPATGWIVGRTPQPAPGLKVNAFAAGLDHPRWLYVLPNGDVLVAETNAPQDRP
                     DDRKGLRAWFEKKIMARAGAGTPSADRITLLRDADGDGIAEFNSVLLSGLHSPFGMAL
                     VGDQLYVADTDALLRFPYHAGDTRITVPGVKVADLPAGTINHHWTKNVIASADGAHLY
                     VSVGSNSNVAENGIEVEQDRARILEIDPASGATRVYATGLRNPVGMAWEPRSGALWTA
                     VNERDELGDDLVPDYMTAVREGAFYGWPYSYYGTHVDERVSPQRADLVAQAVVPDYAL
                     GPHTASLGLTFYSGTLLPQHYRGGAFVGQHGSWNRRQRSGYKVIFVPFADGKPAGMPE
                     DVLTGFIDVEGNAMGRPVGVAVDRRGALLVADDVGGTIWRVTPR"
     gene            3081..3452
                     /locus_tag="VHE37_14590"
     CDS             3081..3452
                     /locus_tag="VHE37_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36815.1"
                     /translation="MERAALADDVVRFIENRIDSVPELEALMLLRDSAPRSWSAGEIA
                     ARIYVEPAQAAALLQNLERKKLLLAAEAGFRFREEDPDAAIFERVAAAYRGNLVAVAS
                     LIHSKSSAAVREFARAFRFKE"
     gene            3457..>3699
                     /locus_tag="VHE37_14595"
     CDS             3457..>3699
                     /locus_tag="VHE37_14595"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HVT36816.1"
                     /translation="MAAAVYIVGALITLVCSVLLLRQYGRTRLPLLLWSGLCFAGLTL
                     SHIVLFIDLIVLPTEVSLYLLRQTITALSMSALLYGL"
BASE COUNT          565 a         1295 c         1281 g          558 t
ORIGIN      
        1 agtgctgcgt gggccatatt cggcgctcta cggaaattcc tccgccggcg tggtgcaggt
       61 gttttccgcc ggcggcaatc cgcccggctt ctactcggcg ttcagcggcg gcagctacgg
      121 cggccggcgc gcggcgtacg cggcgcgcgg cgtcgacggc gcgctggagt atggattgtc
      181 ggccgattac ttcggcaccg acggctaccg cggccacagc gcggcgcagc gctggatcgg
      241 caacggccag ctgcgctacg acttcggcag cggcggcacg ctggaagtcc tcggcagcag
      301 catcgacctg ccggaggcgc aggacccgct gggcctgacc gcgacacagc tggccaccga
      361 ccgcacgcag gccacgctgc ccgcggtgca gttcaacacg cgcaagcgcg tgcaccagta
      421 ccagggcggc ctggtctggg aaaaaaaatt ctccgatgcg ctgagcctgc gcgtgatggg
      481 ctacggcggc caccgcgcca tcggccaggt gctgtcgacg ccggtggcgg cgcagaccgg
      541 cagcccgctc aattccggcg gcgtggtgga cctgggcagc gtgtacgacg gcggcgacgc
      601 gcgcctgcag taccagcttg gcgcgctgca gctcaccggc ggattctcct acgaccgcca
      661 ggaccagcac cgccgcggtt acgagaattt ttccggtgcg cagatgggcg tgcagggcaa
      721 gctgcgccgc gacgaacagg acgacgtgta caacgtcgac gagtacctgc aggccgactg
      781 gcgcttcgcg cagcgctggt cgctgctggc cggcgtgcgc cacagcgagg tgttgttcgc
      841 ctccaacgac gcctacatcg tcgccggcaa cccggacgac ggcggcagcc gcaactacgc
      901 ggcgaccacg ccggtggccg gcctgatgtt ccgcgccagc gatgcgctgc atctgtatgc
      961 gtcgtacggc gcgggcttcg agacgccgac cttcaccgag atggcttacc ggccggacgg
     1021 cagcgccggg ctcaacctcg ggctggacga agcgcgctcg aacaatttcg agatcggcgc
     1081 caagcaccgc gccgacgggc gcagcattga ggccgcattg ttcgaggccg acgcgcgcaa
     1141 tgaactcgcg gtgttcagca acagcaacgg gcgcaccacc tatcacaaca tcgaccgcgc
     1201 gcggcggcgc ggcgcggagc tttccgccag ccaggatttt tcctcgcact ggaatgtgag
     1261 cctggcctac acctgggtgc aggcgcagtt cgaaaccgcg tttgccaact gcgccacgcc
     1321 ggccagctgc accatcaacg gcggcacgcc gattcccggc gtgccgcgca acgatctcta
     1381 caccgagctg cgctggcagg tggcgtcggg ctggcatgcg ggcatcaacg cgcaggcgct
     1441 gtcgcgggtg tatgccgacg acgccggcac gcagcacgct gccggctacg cgctgctcgg
     1501 cgccgacgcc ggctggagcg cgccgcggca cgacgcgcgc ctgtcgctgt acctgcgcgg
     1561 cgagaatctc accggcgcgc gctacgtcgg ctcggtcatc gtcaatgagg ccaacggccg
     1621 ctatttcgag cccgcgccgg gccgcacgtt cttcgccggc gcctcgctgc gctattgaag
     1681 tgaatccgaa ccgcgtggcc cgcgcatagt ccatagcagc atgaatcgcg cgatcctgtt
     1741 gatggcggca ttgctcgcgg ccggcgtcgg cgcggcggac gatgagccgt catcggccac
     1801 gcccggctac ggctacaacc cgccgctgcc ggcgccggcg cattcgctga tccccacggt
     1861 gaacattgcg ccggccaccg gctggatcgt cggtcgcacg ccgcagccgg cgcccgggct
     1921 gaaggtcaac gcctttgccg ccgggctcga ccatccgcgc tggctttacg tgctgcccaa
     1981 cggcgacgtg ctggtggccg agaccaatgc gccgcaggac cggcccgatg accgcaaggg
     2041 gctgcgtgcg tggttcgaga agaagatcat ggcgcgcgcc ggtgcgggca cgcccagcgc
     2101 ggaccgcatc acgctgctgc gcgatgccga tggcgacggc atcgccgagt tcaacagcgt
     2161 gctgctgtcg ggcctgcact cgccgttcgg catggcgctg gtgggcgacc agctctacgt
     2221 ggccgacacc gacgcgctgc tgcgcttccc gtaccacgcc ggcgacacgc gcatcaccgt
     2281 gcccggcgtg aaggtggccg acctgccggc cgggaccatc aaccaccact ggaccaagaa
     2341 cgtcattgcc agcgccgatg gcgcgcacct gtacgtgagc gtgggctcca acagcaacgt
     2401 cgcggagaac ggcatcgagg tggagcagga ccgcgcccgc atcctcgaga tcgatccagc
     2461 cagtggcgca acccgtgttt atgccaccgg cctgcgcaac ccggtgggca tggcgtggga
     2521 gccgcgcagc ggcgcgctgt ggaccgcggt aaacgagcgc gacgagctgg gcgacgacct
     2581 ggtgcccgac tacatgaccg cggtacgcga gggcgccttc tacggctggc cctacagcta
     2641 ctacggcacg cacgtggatg aacgggtgag cccgcagcgc gcggacctgg tggcgcaggc
     2701 cgtcgtgccg gattacgcgc tcggcccgca caccgcctcg ctcgggctca cgttctacag
     2761 cggcacgctg ctgccgcagc attaccgcgg cggcgccttc gtcggccagc acggctcgtg
     2821 gaaccgccgg cagcgctccg gctacaaggt gatcttcgtg ccgttcgcgg acggcaagcc
     2881 ggccggcatg ccggaagacg tgctcaccgg tttcatcgac gtggaaggca acgccatggg
     2941 ccgcccggtg ggcgtggcgg tggaccggcg cggcgcgctg ctggtggccg acgatgtcgg
     3001 cggcacgatc tggcgcgtga cgccgcgcta ggcgatactc gccgcccgcg gtccacggag
     3061 gaccgccaga ccactgcaac atggaacgtg ccgccctggc ggacgacgtc gtccgcttca
     3121 ttgagaaccg catcgattca gtgcccgaac tggaggcgct gatgctgctg cgcgacagcg
     3181 cgccgcgcag ctggagcgcg ggcgagatcg ccgcgcgcat ctatgtcgag ccggcgcagg
     3241 ccgctgcgct gctgcagaac ctcgagcgca agaaactgct gctggcggcc gaggccggat
     3301 tccgcttccg cgaggaagat ccggatgccg ccatcttcga gcgcgtggcc gccgcctacc
     3361 gcggcaacct ggtcgcggtg gccagcctga tccactcgaa gtcttccgcc gccgtgcgcg
     3421 agtttgcgcg cgcgttccgt ttcaaggagt gaagccatgg ccgctgccgt ctacatcgtt
     3481 ggcgcgctga tcacactggt gtgttcggtg ctgctgctgc gccagtatgg gcgcacccgg
     3541 ctgccgctgt tgctgtggag cgggttgtgc ttcgccgggc tgacgctgtc gcacatcgtg
     3601 ctgttcatcg acctgatcgt gctgcccacc gaggtcagcc tgtacctgct gcgccaaacc
     3661 atcaccgcgc tgtccatgtc ggcgctgctg tacggcctg
//
LOCUS       DASZZC010000690         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_310522,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000690 DASZZC010000000
VERSION     DASZZC010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310522"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1516
                     /locus_tag="VHE37_14600"
     CDS             62..1516
                     /locus_tag="VHE37_14600"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVT36817.1"
                     /translation="MKLHASLVVCTAVAGVLLPAGLLSAQQVITGPAAFADWSQQKPG
                     TLRKITAADLPAPFKTESVDNGPTVISRPKDAWPIAPPGFKVELYAGGDAATPMQRAD
                     DVRKIYGPTSGTFVMPRIIHTAPNGDLFVSDSQAGSIFVLRGVTAEGKAQTVSQFASG
                     LDHPFGIAFYPAGSDPKWIYVGTATQIVRFAYKPGDLKAESNTPQVIIPDIPGYAQLR
                     GGGHWTRDVIFTADDKHMLISVGSGSNIDDPDTHPREFHRADVLEYTPEGKFVEVYAS
                     GIRNCVGEAINPVTKQLWCSTNERDQLGSNLPPDYVTHIEEGGFYGWPWYYIGDHKDP
                     RLPLPCANGSGPNPQLTAPLSALDAASCKRIDIGSKVLTPDVLVQPHMASLGMTFYPG
                     HSANFPAEYEGDGFAAEHGSWNRARRGGYEVIMFPMKDGKALGEYEDFLTGFVTADGQ
                     VWGRPVGVTVGHDGALYVTDDGSRSIWRVVYTGK"
BASE COUNT          332 a          544 c          486 g          320 t
ORIGIN      
        1 atcggtgtaa cggctgcttg cccgttcgcc tgaaatccaa aaaattccga ggcatcttcc
       61 catgaaactc catgcttccc ttgtcgtttg tactgctgtg gccggcgtgc tgctgcctgc
      121 cggattgctc tctgcacagc aggtcatcac cgggcccgcc gcgtttgccg actggagcca
      181 gcagaagccg ggcaccctgc gcaagattac tgcggccgat ctgcccgcac cgttcaagac
      241 agaatcggtg gacaacggcc ctacggtgat cagccggccc aaagacgcct ggcccattgc
      301 accgccggga ttcaaggttg aactctatgc cggcggcgat gcggccacac ccatgcagcg
      361 cgcggacgat gtacgcaaga tctacgggcc tacctcaggc acattcgtca tgccgcgcat
      421 cattcatacc gcgcccaacg gcgatctgtt cgtctcggac tcgcaggccg gatcgatctt
      481 tgtgctgcgc ggcgtaactg cagagggcaa ggcacagacc gtcagccagt tcgccagcgg
      541 cctcgaccac cccttcggca tcgcgttcta tccggcgggc tccgatccga agtggatcta
      601 tgtcggcacg gccacacaga ttgttcggtt cgcctacaag ccgggcgacc tgaaagccga
      661 aagcaatacg ccgcaagtca tcattccgga catcccgggc tacgcgcagc tgcgcggcgg
      721 cggccactgg acacgcgacg ttatcttcac cgccgacgac aagcacatgc tcatctcggt
      781 gggcagcggc tccaacatcg acgatccgga cacgcacccg cgcgagttcc accgcgccga
      841 tgtgctcgag tacacgcccg agggcaagtt cgtcgaggtc tacgcaagcg gcatccgcaa
      901 ctgcgtgggc gaggccatca acccagttac caaacagctc tggtgctcca ccaatgagcg
      961 cgaccagctg ggcagcaatc tgccgcccga ctacgtaacg catatcgaag agggcggctt
     1021 ctatggctgg ccctggtatt acatcggcga ccacaaggat ccgcgtttgc cgctgccctg
     1081 cgccaatggc tccggcccga atccgcagct caccgcgccg ctctctgcgc tcgacgcggc
     1141 aagctgcaag cgcatcgata tcggctcgaa ggtgctgact cccgatgtgc tggtgcagcc
     1201 gcacatggcg tcgctgggca tgactttcta tcccggccac tcggctaact tcccggctga
     1261 gtatgaaggc gatggctttg ccgccgagca cggctcgtgg aaccgcgcgc gccgcggcgg
     1321 ctatgaagtg atcatgttcc cgatgaagga cggcaaggca ctgggcgaat atgaggactt
     1381 cctgaccggc tttgtcacgg ccgacggcca ggtgtgggga agaccagtcg gcgtcacggt
     1441 tggccacgac ggcgcgcttt atgtcaccga cgacggctcg cgcagcatct ggcgcgtggt
     1501 ttacaccggc aagtaagttc actccttttt aagaagccga gggcggctcg caaagcctca
     1561 acaggggcct tgcgagccgc cttttttatg cgcaggattt ccgcaagcca tagaaataca
     1621 ttgacttatt tggagatatt tgatataata ctaaagtgct tttcagcacg gaacctatct
     1681 tc
//
LOCUS       DASZZC010000691         7905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_55755,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000691 DASZZC010000000
VERSION     DASZZC010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55755"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VHE37_14605"
     CDS             <1..442
                     /locus_tag="VHE37_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36818.1"
                     /translation="ALESFPFPHCVENCGRLEHDPATGLSGRERVLRLMDTADALLLL
                     HGNDGFCEEYFPSKLYEYLWTQRPILALVHHNPDLENFLRELGHEPAPAEDAQAIGEV
                     LTRLMQRWDNDDLRDNGRVSPYSTQRSVEQLVNWGREISAPARR"
     gene            complement(608..1603)
                     /locus_tag="VHE37_14610"
     CDS             complement(608..1603)
                     /locus_tag="VHE37_14610"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVT36819.1"
                     /translation="MHAISVLGAGSFGTALAIHLARRRSATLLWGRDAARMAAMQAAR
                     ENTRYLPGCPFPAPLTATADLADAVAQGEDLLIATPSDALRGTLDAVRPLLRAGQGIA
                     CACKGLEPGSGLLVHEVVRDALGEVPLVVISGPTFAKELGMGLPTSVTVASRDSAFAD
                     KIAHRLHGDGFRAYTADDLVGVEIGGAAKNVMAIAVGMADGLNLGANTRAAVITRGLA
                     EIMRLSEALGARAETLMGLAGLGDLVLTCTDNQSRNRRLGLLLAQGKSVDAARAEIGQ
                     AVEGVKAAPEVARLAKKHGVEMPITEAVAGVLAGRLTPVQAVMLLATRPRKAESD"
     gene            complement(1610..2080)
                     /gene="secB"
                     /locus_tag="VHE37_14615"
     CDS             complement(1610..2080)
                     /gene="secB"
                     /locus_tag="VHE37_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HVT36820.1"
                     /translation="MSDAPAQRQLLLHKIYLKDASVEVPKAPSVYTRPWQPQMDVQVN
                     TGITQVNGDNWQVLLGVTVTAKLGDDVAFLIEVHQAGIFGLPGFPDGEREAVLAGHCA
                     GVVFPYAREAISELVQRAGFPAFLLQPINFDAVYAEHNARLKATQAAPADARPH"
     gene            complement(2077..2337)
                     /gene="grxC"
                     /locus_tag="VHE37_14620"
     CDS             complement(2077..2337)
                     /gene="grxC"
                     /locus_tag="VHE37_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HVT36821.1"
                     /translation="MKKIRMYTTHFCPFCVMAKRLLTQKGVPYEEVAVDTDASRRDEM
                     MQLTGRRTVPQIFVGETHVGGFDDLAALDRAGKLDPLLKENS"
     gene            complement(2353..2775)
                     /locus_tag="VHE37_14625"
     CDS             complement(2353..2775)
                     /locus_tag="VHE37_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVT36822.1"
                     /translation="MQQLLDFFHHHLPLFLALAVVLTLLVANEVHGNLTGGPRLGPYE
                     AVRLINDRDPLILDLRPAGDFKRGHLLGAQNFPMLKLAERADEISRDKSRPVIVYDAL
                     GSAHGAVDALRKAGYSAVYPLRGGINAWLGANLPVTAK"
     gene            2831..4012
                     /locus_tag="VHE37_14630"
     CDS             2831..4012
                     /locus_tag="VHE37_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVT36823.1"
                     /translation="MTRLSSLLVLVAASLPLAASRADEAAAPAAAAQPSAEQSQQQLE
                     RVRTRIEAVKAKIDADRRRRDTLQEQVQKAEQDVAQAQGQVAGVEHDIALAQEKVRIA
                     QGEVARSQAVLDAQRAALGRQLRAAYLVGERGEAKLLLNQEDAQKLGRVLIDYDYLNR
                     ARTLRVQRIMQQLDLLEGFRVRIRSQLDELTALKAQQQATLATLETARQQRTSALGKL
                     AERIADEVEELKTLKTNEKETQNLLTSLQDVLADIPMDLGNAPPFAQLKGRLPWPLHG
                     LLLADYGSSKLGGKLTWNGLWIGTDPGTPVHAIARGRVAYVGWLNRYGLMVILEHDGG
                     WFSLYGHDDAANCSAGQWVNAGDTIATAGNTGGAEQTGLYFEIRNGADPVNPNDWLAR
                     Q"
     gene            4148..5497
                     /locus_tag="VHE37_14635"
     CDS             4148..5497
                     /locus_tag="VHE37_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVT36824.1"
                     /translation="MSILSRPSVALAAGITIGISAALTHGVLAEREKPEPVPLKDLQT
                     FVEILNHVKANYVEPVTDQQLLENAVRGMIAGLDPHSSYLDKDEYKDMNVITTGKFGG
                     LGIEVQLQNGFVRVVSPIDDTPAARAGIQPGDYIVKIDDTPVKGMSLNDAVTKMRGEA
                     GSKITLTVVRDGAAAPMVLEMKRDIINVSSVKSKVLEPGLDYIRISNFTTETGASLEK
                     ELAKLKKQGEIKGLVLDLRNNPGGVLDAAVKVSDAFLEKGPIVSIKGRNPDANREFDA
                     TGGDLIGGKPMVVLINGGSASASEIVAGALQDQHRAILVGSRSFGKGSVQTIMPLQND
                     AAIKLTTARYYTPSGRSIQAEGIQPDVELKALKVAHDDSADEIEPIKEADLKGSLVNE
                     NAAKKVTVDKKDDKTITVEIKDNKDAKDKEKDKPLAETDYGLYEALNLLKGLVIAQR"
     gene            5620..6465
                     /locus_tag="VHE37_14640"
     CDS             5620..6465
                     /locus_tag="VHE37_14640"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HVT36825.1"
                     /translation="MPSGFSSRRWARACAVACCAWSAATLAAPAVSIVIDDLGDRRVE
                     GMEVVALPGPLGCAVLPATPYGAALARAAHGTEKEVLLHFPLQPISGKAHPLAITEHS
                     SRGELQTQLRDDLAALPYVVGVNTHQGSLLSQKVPPMHWLMSEIKAHGGLYFIDSYTT
                     PNSAALRAAQDWGLPTTRREVFLDDVRTDAEITAQFNRLLALARRDGTSLAIGHPYPE
                     TIAVLRRELPRLSAAGVALVAPSELIRLRGSPRPHEHDPILLKLTPAMAAAAPTPESA
                     PPGTP"
     gene            complement(6588..>7905)
                     /locus_tag="VHE37_14645"
     CDS             complement(6588..>7905)
                     /locus_tag="VHE37_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HVT36826.1"
                     /translation="EIIEQSFGKKIEELYAEFDETPLASASVAQVHAARMHATAPGEP
                     GFEVVVKVIRPGVEQTIGDDIDLLHTLAELAEKFSDTARRIKPRAIVAEYEKVILDEL
                     DLMREGANASLLRRNWLGSDLIYHPLVLWDWTRPRVLTMERLHGISIRELPRLREMGV
                     DFKVLAERGVEIFFKQTFRDNFFHADMHPGNIFVDASDPKKPGYLAVDFGIVGTLSPH
                     DQRYLAENFLAFFNRDYRRVAELHVESQWVPEGTRVEEFEAAIRTVCEPIFQRPLKDI
                     SFGFFLLRLFQIARRFNYQVQPQLVLLQKTLLQIEGLGRQLYDELDLWKTAKPIMEDW
                     MKNRLGPMATLERMRTQMPALAETLPELAQRALKQLEKGDGLGAGNRRELLALRDEIR
                     DANRRTTHTVIGAALLIGAVLLWSGAAPWYAIAALAAGGAWFALRR"
BASE COUNT         1345 a         2694 c         2641 g         1225 t
ORIGIN      
        1 cgcgctggag agctttccgt tcccgcattg cgtggagaac tgcgggcggc tggagcacga
       61 tcccgccacc ggactgtcgg gccgcgagcg cgtgctgcgg ctgatggaca ccgccgacgc
      121 gctgctgctg ctgcacggca acgacggctt ctgcgaggaa tattttccgt cgaagctcta
      181 cgaatacctg tggacgcagc ggccgatcct cgcgctggtg catcacaatc ccgacctgga
      241 aaatttcctg cgcgagctcg gccacgagcc ggcgccggcg gaagacgcgc aggcgatcgg
      301 cgaggtgctc acgcgcctga tgcagcgctg ggacaacgac gacctgcgcg acaacggccg
      361 cgtctcgccg tattccacgc agcgctcggt ggaacagctg gtgaactggg gacgcgagat
      421 cagcgcacca gcccgccgct gatgcgtacg cggaatttcc gcagcgcttt tcctttcacc
      481 gcaccgccgg ttttgatgtt atataagata gtataattat agaaatatat aataattaat
      541 aacatcaaaa atgatcatgc ggtcggcggt ttttcctcga ttccggcacc gctggcgcga
      601 tccgcgctca atcggactcg gccttgcgcg ggcgcgtggc cagcagcatc accgcctgca
      661 ccggcgtgag ccggccggcg agcacgccgg cgacggcctc ggtgatcggc atctccacgc
      721 cgtgcttctt cgccagccgc gccacttccg gcgcggcctt gacgccttcc accgcctggc
      781 cgatttcggc gcgcgccgcg tccaccgact tgccctgcgc cagcagcagg cccaggcgcc
      841 ggttgcgcga ctggttgtcg gtgcaagtga ggacgaggtc gcccaggccg gccagaccca
      901 tcagcgtttc cgcgcgcgca cccagcgctt cggacagccg catgatttcc gccagcccgc
      961 gcgtgatcac ggccgcgcgc gtattggcgc cgagattgag tccgtcggcc atgcccacgg
     1021 caatcgccat cacgttcttg gccgcaccgc cgatctccac gccgaccagg tcgtccgcgg
     1081 tataggcgcg gaagccgtcg ccatgcaggc gatgcgcgat cttgtcggcg aaggcgctgt
     1141 cgcgcgaggc caccgtcacc gaggtcggca agcccatgcc cagttccttg gcgaaggtcg
     1201 gcccggagat caccaccagc ggcacttcgc ccagcgcatc gcgcaccacc tcgtgcacca
     1261 gcaggccgct gccgggttcc agtcccttgc acgcgcaggc gatgccctgc cccgcgcgca
     1321 gcagcggccg tacggcgtcg agggtgccgc gcagcgcgtc gctgggcgtg gcgatgagca
     1381 ggtcctcgcc ctgcgccaca gcgtcggcca ggtcggccgt cgccgtcagc ggcgcgggaa
     1441 acgggcagcc tggcaggtag cgcgtgttct cgcgcgcggc ctgcatggct gccatgcgcg
     1501 cggcatcgcg gccccacagc agcgtggcgc tgcgccggcg cgcaaggtgg atcgccagcg
     1561 cggtgccgaa ggagcccgcg ccgaggaccg agatggcatg catcggggct tagtgcgggc
     1621 gcgcgtccgc gggcgcggcc tgcgtggcct tgagccgcgc gttgtgctcg gcgtagaccg
     1681 catcgaagtt gatcggctgc agcaggaacg cggggaagcc cgcgcgctgc accagctcgc
     1741 tgatcgcttc gcgcgcatac ggaaacacca cgccggcgca atggccggcc agcaccgctt
     1801 cgcgttcgcc gtcggggaac cccggcaggc cgaagatgcc ggcctggtgc acttcgatca
     1861 gaaaggccac gtcgtcgccc agcttggcgg tgacggtgac gcccagcagc acctgccagt
     1921 tgtcgccgtt tacctgggtg atgccggtgt tgacctgcac gtccatctgc ggctgccacg
     1981 ggcgcgtgta caccgacggc gccttaggca cttccaccga ggcatccttg agatagatct
     2041 tgtgcagcag cagctggcgt tgcgcgggtg cgtcgctcat gagttttcct tgagtaatgg
     2101 gtcgagcttg ccggcgcgat cgagcgcggc caggtcgtcg aaaccgccga catgggtttc
     2161 gccgacgaag atctgcggca ccgtgcggcg gccggtcagc tgcatcatct cgtcgcggcg
     2221 cgaggcgtcg gtgtccaccg cgacctcttc gtagggcacg cccttctgtg tcagcagccg
     2281 cttggccatc acgcagaacg ggcagaaatg cgtggtgtac atgcggattt ttttcatgac
     2341 cgcgggctca actcacttcg cggtgaccgg caggttggcg ccgagccagg cgttgatgcc
     2401 gccgcgcagc gggtacaccg cgctgtagcc ggccttgcgc agcgcgtcga ccgccccgtg
     2461 cgcgctgccc agcgcgtcgt agacgatcac cgggcggctc ttgtcgcggc tgatctcgtc
     2521 ggcgcgctcg gccagcttga gcatgggaaa attctgcgcg ccgagcaggt ggccgcgctt
     2581 gaagtcgccg gccgggcgca ggtcgagaat cagcgggtcg cggtcattga tcaggcgcac
     2641 cgcctcgtac gggcccaggc gcggaccgcc ggtgagattg ccgtgcacct cgttggccac
     2701 cagcagcgtg agcaccacgg ccagcgccag gaacagggga agatgatggt gaaagaagtc
     2761 gagcagctgc tgcatggtga agacgttttc cgcaggaaat ccgggaggca aaaccctcta
     2821 tgattatcgc atgacccgcc tgtcatcgct cctcgtgctg gttgcggcaa gcctgccgct
     2881 ggccgccagc cgcgccgacg aggccgccgc cccggccgcc gccgcgcagc ccagcgcgga
     2941 gcagagccag cagcagctgg agcgcgtgcg cacgcgcatc gaggcggtga aggccaagat
     3001 cgacgccgac cgccgccggc gcgacacgct gcaggagcag gtgcagaaag ccgagcagga
     3061 cgtggcgcag gcgcagggcc aggtggccgg cgtcgagcac gacattgcgc tggcgcagga
     3121 gaaggtgcgc atcgcccagg gcgaggtggc gcgctcgcag gcggtgctcg acgcgcagcg
     3181 cgcggcgctc ggccgccagc tgcgtgcggc gtacctggtg ggcgaacgcg gcgaagccaa
     3241 gctgctgctc aaccaggagg acgcgcagaa gctgggccgc gtgctgatcg actatgacta
     3301 tctcaaccgc gcgcgcaccc tgcgcgtgca gcggatcatg cagcagctcg acctgctcga
     3361 aggattccgc gtgcgcatcc gctcgcagct cgacgagctc accgcgctga aggcgcagca
     3421 gcaggccacg ctggcgacgc tggagacggc acgccagcag cgcacctcgg cgctgggcaa
     3481 gctggccgag cgcatcgccg acgaggtcga ggagctgaag accctcaaga ccaacgagaa
     3541 ggaaacgcag aacctgctca cctcgctgca ggacgtgctg gccgacatcc cgatggacct
     3601 gggcaacgcg ccgccgttcg cgcagctgaa gggccgcctg ccgtggccgc tgcacggcct
     3661 gctgctggcc gactacggca gttccaagct gggcggcaag ctgacctgga acggcctgtg
     3721 gatcggcacc gatcccggca cgccggtgca cgccatcgcg cgcgggcgcg tggcctacgt
     3781 cggctggctc aaccgctatg gcctgatggt gatcctggag cacgacggcg gctggttctc
     3841 gctgtacggc cacgacgatg ccgccaactg cagcgccggc cagtgggtca acgccggcga
     3901 caccatcgcc accgccggca acaccggcgg cgccgagcag accggcctgt acttcgagat
     3961 ccgcaacggc gccgacccgg tcaatcccaa cgactggctg gcgcgccagt agtcccaccc
     4021 ggcgtagaga aaagcactcc gtcccgcccg tcgggaaccg gcgccagcgc tgctggtcta
     4081 tgtgctaagg tgacctgcgc ttctggcgcc cttcgcctga ctcttccctt ctccgttgaa
     4141 ccgaataatg tccatcctgt cccgtccctc ggtcgcgctg gcggccggca tcaccatcgg
     4201 catctccgcc gccctcaccc atggcgtgct cgccgagcgc gagaagccgg agccggtgcc
     4261 gctgaaggac ctgcagacct tcgtcgagat cctcaaccac gtgaaggcca actacgtgga
     4321 gccggtcacc gaccagcagc tgctggagaa cgcggtgcgc ggcatgatcg ccgggctcga
     4381 tccgcactcg tcctacctgg acaaggacga gtacaaggac atgaacgtca tcaccaccgg
     4441 caagttcgga ggcctgggca tcgaggtgca gctgcagaac ggcttcgtgc gcgtggtctc
     4501 gcccattgac gacacgccgg cggcgcgcgc cggcatccag ccgggcgatt acatcgtcaa
     4561 gatcgacgac acgccggtga aaggcatgtc gctcaacgac gcagtgacca agatgcgcgg
     4621 cgaggccggc agcaagatca ccctcactgt ggtgcgcgac ggcgccgccg cgccgatggt
     4681 gctggagatg aagcgcgaca tcatcaacgt gtcgtcggtg aagagcaaag tgctggagcc
     4741 ggggctggac tacatccgca tcagcaactt caccaccgag accggcgcct cgctggagaa
     4801 ggagctggcc aagctgaaga aacagggcga gatcaagggc ctggtgctcg atctgcgcaa
     4861 caacccgggc ggcgtgctcg acgccgcggt caaggtgagc gacgccttcc tggagaaggg
     4921 cccgatcgtg tcgatcaagg gccgcaatcc cgacgccaac cgcgaattcg acgccaccgg
     4981 cggcgacctc atcggcggca agcccatggt ggtgctgatc aacggcggct ccgcctctgc
     5041 ttcggaaatc gtagcaggcg cgctgcagga ccagcaccgc gccatcctgg tcggctcgcg
     5101 cagcttcggc aagggctcgg tgcagaccat catgccgctg cagaacgacg cggcgatcaa
     5161 gctgaccacc gcgcgctact acacgccgtc gggccgcagc atccaggccg agggcatcca
     5221 gcccgacgtc gagctgaagg cgctgaaggt ggcgcacgat gacagcgccg acgagatcga
     5281 gccgatcaag gaagccgacc tcaagggcag cctggtcaac gagaacgcgg cgaagaaggt
     5341 cacggtggac aagaaggacg acaagaccat caccgtggag atcaaggaca acaaggacgc
     5401 caaggacaag gagaaggaca agccgctggc cgagaccgac tacggcctgt acgaggcgct
     5461 caacctgctc aagggcctgg tcatcgcgca gcgttgaacg cgcccggctg acgttggcgt
     5521 cagcgtagga attttcggaa agtgccctaa agccctcccg cgcacggcgc gggagggttt
     5581 attatttccg acagtttcac acacgcagga tcgaagggaa tgccatcggg tttttcatcg
     5641 cgccgctggg cgcgcgcatg cgccgtggcg tgctgcgcgt ggagcgctgc cacgctggcg
     5701 gcgccggcgg tgtccatcgt catcgatgac ctgggcgacc gccgcgtgga aggcatggaa
     5761 gtggtggcgc tgcccggccc gctcggctgc gcggtgctgc cggccacgcc ctacggcgcg
     5821 gcgctggcgc gcgccgcgca cggcacggag aaggaagtgc tgctgcattt cccgctgcaa
     5881 cccatctccg gcaaggcgca cccgctggcc atcaccgagc attcgtcgcg cggcgaactg
     5941 cagacgcaac tgcgcgatga cctcgccgcg ctgccctacg tggtcggcgt gaacacgcac
     6001 cagggcagcc tgctgtcgca gaaggtgccg ccgatgcact ggctgatgtc ggagatcaag
     6061 gcgcacggcg ggctgtattt catcgacagc tacaccacgc ccaacagcgc ggcgctgcgc
     6121 gcggcgcagg actggggcct gccgaccacg cggcgcgaag tgttcctcga cgatgtgcgc
     6181 accgatgccg agatcaccgc gcagttcaac cgcctgctgg cgctggcgcg gcgcgacggc
     6241 acttcgctgg ccatcggcca cccgtacccg gaaacgatcg cggtgctgcg ccgcgaactg
     6301 ccgcggctca gcgccgccgg cgtcgcgctg gtggcgccgt cggaactgat ccgcctgcgc
     6361 ggctcgccgc gcccgcatga gcacgatccc atcctgctca agctcacgcc ggcgatggcc
     6421 gccgcggcgc cgacgccgga atccgcgccg ccgggcacgc cgtaggcgac gctttttttt
     6481 ctcaccgcac tgctatttct tgtgttataa attattgtct aaatatttaa tcagataatt
     6541 ttttataaca tcgtcagggc aggtgcggtt ggccgaataa acagcgctca gcgccgcagc
     6601 gcgaaccacg cgccgccggc agccagcgcc gcaatcgcgt accacggcgc cgcaccgctc
     6661 cacagcagca cggcgccgat cagcagcgct gcgccgatca ccgtgtgcgt ggtgcggcgg
     6721 ttggcgtcgc ggatctcgtc gcgcagcgcc agcagttcgc gccggttgcc cgcgcccagg
     6781 ccgtcgccct tctccagctg cttgagcgcg cgctgcgcca gttccggcag cgtttccgcc
     6841 agcgccggca tctgcgtgcg catgcgctcg agcgttgcca tcgggcccag gcggttcttc
     6901 atccagtctt ccatgatcgg cttggccgtc ttccacaggt ccagctcgtc gtagagctgg
     6961 cggcccaggc cctcgatctg cagcagcgtc ttctgcagca gcaccagctg cggctgcacc
     7021 tggtagttga agcgccgcgc gatctggaac aggcgcagca ggaagaagcc gaacgaaatg
     7081 tccttcagcg gccgctggaa gatcggctcg cacacggtgc ggatggccgc ctcgaattcc
     7141 tctacgcgcg tgccctcggg cacccattgg ctttccacgt gcagctcagc cacgcgccgg
     7201 tagtcgcggt tgaagaaggc caggaaattt tccgccaggt aacgctggtc gtgcggtgac
     7261 agcgtgccga cgatgccgaa atccaccgcg aggtaaccgg gcttcttcgg atctgacgcg
     7321 tcgacaaaga tgttgccggg gtgcatgtcc gcgtggaaga agttgtcgcg gaaggtctgc
     7381 ttgaagaaga tctccacgcc gcgctcggcc agcaccttga agtccacgcc catctcgcgc
     7441 agccgcggca gctcgcggat ggagatgccg tgcaggcgtt ccatggtcag cacgcgcggg
     7501 cgcgtccagt cccacagcac cagcgggtgg tagatcaggt cggagcccag ccagttgcgc
     7561 cgcagcagcg aggcgttggc gccttcgcgc atcaggtcca gctcgtcgag gatgactttc
     7621 tcgtactcgg cgacgatcgc gcgcggcttg atgcgccgcg cggtgtcgga gaatttttcc
     7681 gcgagctccg ccagcgtgtg cagcagatcg atgtcgtcgc cgatggtctg ctcgaccccg
     7741 ggccggatca ccttgaccac cacctcgaag cctggctcgc ccggcgccgt cgcatgcatg
     7801 cgcgccgcgt gcacctgcgc caccgaggcg gaggccagtg gcgtctcgtc gaactcggcg
     7861 tagagctcct cgattttctt tccgaacgac tgctcgatga tctcg
//
LOCUS       DASZZC010000692         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1022948,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000692 DASZZC010000000
VERSION     DASZZC010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022948"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VHE37_14650"
     CDS             complement(<1..599)
                     /locus_tag="VHE37_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36827.1"
                     /translation="MGYASVFRPGLFQGQTIIVTGGGSGIGRCVAHELAALGAKVLIT
                     GRKLEKLEKTAAEIREDGGTVAHYAFDIRDEDAVKNAIKQMLADHGQIHGLVNNAGGQ
                     YPMPLAMINKKGWEAVVANNLTGGFLVARELYNQCLKKNGGAIVNMIADMWGGMPGMG
                     HSGAARAGMLNFTETAAVEWGSSGVRVNAVAPGWIASSG"
     gene            complement(826..1764)
                     /locus_tag="VHE37_14655"
     CDS             complement(826..1764)
                     /locus_tag="VHE37_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HVT36828.1"
                     /translation="MSKILVVAEHANGKLNAGVAKVMAAAQKIGGDVDVVVYSTDGSA
                     VAAEAAKLAGVSKVLQADAAANDHALAVTLAPQIVDLAGKGYTHVLLPGTTFGKDIAP
                     RVAAKLGVPQVSDVMGVQSANTFDRPTYAGNAITTVETPAGKTVVATVRLASFTAVAS
                     SGSAAVEKISTNAATPAHTKFVSLAAAKADRPDLQSANKVVSGGRALGSADAFKIIYA
                     LADKIGAGVGASRAAVDSGYVPNDMQVGQTGKIIAPELYIAIGISGAIQHLAGIKDAR
                     TIVAINKDAEAPIFEIADFGLVGDLFTIVPELTGKL"
     gene            complement(1764..2510)
                     /locus_tag="VHE37_14660"
     CDS             complement(1764..2510)
                     /locus_tag="VHE37_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HVT36829.1"
                     /translation="MKALVSVKRVVDYNVRVQIKPDGSGVVTDGVKHSMNPFDEIAME
                     EAVRQKEKGKITEIIAVTIGGDKCEETLRSALAFGADRAVHVKEAGEVQPLTAARALA
                     AVFKKEGANVFFCGKQAIDDDANQTGQMVAALLDLPQATFASKVELDGGKATVTREID
                     AGLETLEVTLPAVITTDLRLNEPRYLKLPDIMKAKKKPVEATSLSALGVEAAASLKTV
                     KTTAPSGRSKGVMVKTVDELVSALKAKGLV"
     gene            complement(2584..>3473)
                     /locus_tag="VHE37_14665"
     CDS             complement(2584..>3473)
                     /locus_tag="VHE37_14665"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002114676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HVT36830.1"
                     /translation="NNQILVGLIVDLSYQNPYLSPFDEFQRFKHHPKIRQYLEGGKRV
                     SYGARAICKGGYNSLPKPTFPGGMLVGCDWGTMNFAKIKGSHTAMKSGMLAAEAVAEA
                     LIGGAQGGDELASYPEKFKASWLHEELHGSRNFGVWLHKFGAIIGGGINYIEQNWFGG
                     KVPWTVHDLKPDYASLKLAAESQKIDYPKPDGKLSFDKLSSVFVSSTNHEEEQPSHLK
                     LADPSIPLSKNLPLYAEPAQRYCPAGVYEVVGEGDAKRFQINAQNCVHCKTCDIKDPA
                     QNITWVAPEGGGGPNYANM"
BASE COUNT          547 a         1161 c         1079 g          686 t
ORIGIN      
        1 atgccgctcg acgcgatcca gcccggcgcc actgcgttga cacgcacgcc ggaggagccc
       61 cactccaccg ccgccgtctc ggtgaaattc agcatgcccg cgcgggccgc gccgctgtgg
      121 cccataccgg gcatgccgcc ccacatgtcc gcgatcatgt tgacgatggc gccgccgttc
      181 ttcttcaggc actggttgta cagctcgcgc gccaccagga aaccgccggt gagattgttg
      241 gccaccaccg cttcccagcc cttcttgttg atcatggcca gcggcatcgg gtactggccg
      301 ccggcgttgt tgaccagccc gtggatctgg ccgtgatccg ccagcatctg cttgatggcg
      361 ttcttcaccg catcctcgtc gcggatatcg aacgcgtagt gcgcaacggt gccgccgtcc
      421 tcgcggattt ccgccgcggt cttttccagt ttctcgagct tgcgcccggt gatcagcacc
      481 ttggcgccga gcgcggcgag ttcatgcgcc acgcagcggc cgatgccgga gccgccgccg
      541 gtgacgatga tggtctggcc ctggaacagg ccgggacgga ataccgatgc gtagcccatg
      601 tggcactcct cgatgtgcaa ccagaaaaag cggcctgcaa aacgtccacc gcccccgcag
      661 tcttgatgtt ataaattgta atgtgaaaaa taaatcacac tacattttat aacacgcaaa
      721 accggggggc ggtggaggcc tttcgtcatg cccgcgcagc gggaatccag tttttttgct
      781 gcaacctcga aagcaaactg gccccgcctg cgcggggatg acagctcaaa gcttgcccgt
      841 cagctccggc acgatggtga acaggtcgcc gaccaggccg aagtcggcga tctcgaagat
      901 cggcgcttcg gcgtccttgt tgatggcgac aatcgtgcgc gcgtccttga tgccggccag
      961 gtgctggatg gcgcccgaga tgccgatggc gatgtacagc tccggcgcga tgatcttgcc
     1021 ggtctgtccc acctgcatgt cgttgggcac gtagccggag tcgaccgccg cgcgcgaagc
     1081 gccgacgccc gcgccgatct tgtcggccag cgcgtagatg atcttgaacg cgtcggcact
     1141 gcccagcgca cggccaccgg agacgacctt gttcgcggac tgcaggtcgg gacgatccgc
     1201 cttggccgcg gccagcgaga cgaatttcgt atgcgccggc gtcgcagcgt tggtggaaat
     1261 cttctcgacc gctgcgctgc ccgaggacgc gaccgcggtg aacgaggcca ggcgcacggt
     1321 ggcgacgacg gtcttgcccg ccggcgtttc caccgtggtg atcgcgttgc cggcgtaagt
     1381 cggacgatcg aaagtattgg cggactgcac gcccatcacg tcggacacct gcggcacgcc
     1441 cagcttggcg gcgacgcgcg gcgcaatgtc cttgccgaag gtggtgcccg gcagcagtac
     1501 gtgcgtgtag cccttgccgg ccaggtcgac gatctgcggc gccagcgtta ccgccagcgc
     1561 gtgatcgttc gccgcggcgt cggcctgcag caccttgctc acgccggcca gcttggccgc
     1621 ttccgcggca accgcggaac catcggtgga ataaacgact acatcgacgt cgccaccaat
     1681 cttctgcgcg gcggccatca ccttggccac gccggcgttg agcttgccgt tggcgtgctc
     1741 tgcaacaacc aggatcttgc tcatcagacc agcccctttg ccttcaatgc ggaaaccagt
     1801 tcatccaccg tcttcaccat cacgcccttg ctgcggcccg acggcgcggt ggtcttcacc
     1861 gtcttcagcg aggcggccgc ttcgacgccg agcgccgaca gcgaggtcgc ttcaaccggc
     1921 ttcttcttcg ccttcatgat gtccggcagc ttcaggtaac gcggctcgtt caggcgcagg
     1981 tcggtggtga tcaccgccgg cagcgtgact tccagcgtct ccaggccggc gtcgatttcg
     2041 cgggtcaccg tggccttgcc gccgtccagt tcaaccttcg acgcgaacgt tgcctgcggc
     2101 aggtcgagca gcgcggccac catctggccg gtctggttgg cgtcgtcgtc gatcgcctgc
     2161 ttgccgcaga agaacacgtt ggcgccttcc ttcttgaaca ccgcggccag cgcgcgcgcg
     2221 gcggtcagcg gctgcacttc accggcttcc ttcacgtgca ccgcgcggtc ggcaccgaac
     2281 gcgagcgccg agcgcagcgt ctcttcgcac ttgtcgccgc cgatggtcac ggcaatgatc
     2341 tcggtgatct tgcccttttc cttctggcgc accgcctctt ccatggcgat ctcgtcgaac
     2401 gggttcatcg agtgcttgac gccgtcggtg acgacaccgg agccatcggg cttgatctgc
     2461 acgcggacgt tgtagtccac gacgcgcttg acgctgacta gtgctttcat aactctcttg
     2521 gttccctctc aaatcaaaat caaaactggt tccccgcctg cgcggggatg acgccggacg
     2581 gcgtcacatg tttgcgtagt tgggcccgcc gccgccctcg ggcgccaccc aggtgatgtt
     2641 ctgcgccggg tccttgatgt cgcaggtctt gcagtgcacg cagttctgcg cgttgatctg
     2701 gaagcgcttc gcatcgccct cgcccaccac ctcgtacacg ccggccgggc aatagcgctg
     2761 cgcgggctcg gcatacagcg gcaggttttt tgagagcggg atcgacggat cggccagctt
     2821 gagatgcgag ggctgctctt cctcgtggtt ggtgctggac acgaacaccg acgagagctt
     2881 gtcgaacgac agctttccgt ccggcttggg gtagtcaatc ttctgcgact cggccgccag
     2941 cttgagcgag gcgtagtccg gcttgagatc atgcacggtc cacggcacct tgccgccgaa
     3001 ccagttctgc tcgatgtagt tgatgccgcc gccgatgatg gcgccgaact tgtgcagcca
     3061 cacgccgaag ttgcggctgc catgcagttc ttcgtgcagc cagctggcct tgaacttctc
     3121 cgggtagctc gccagttcgt cgccgccctg ggcgccgccg atcagcgctt ccgccaccgc
     3181 ttccgcggcg agcatgccgc tcttcatcgc ggtatggctg cccttgatct tggcgaaatt
     3241 catcgtgccc cagtcgcaac ccaccagcat gccgccgggg aaggtgggct tgggcagcga
     3301 gttgtagccg cccttgcaga tggcgcgcgc gccgtagctc acgcgcttgc cgccctcgag
     3361 gtactggcgg attttcgggt gatgcttgaa gcgctggaac tcgtcgaacg gcgacaggta
     3421 cgggttctgg tacgacaggt cgacgatcag gccgaccaat atctggttgt tct
//
LOCUS       DASZZC010000693         9627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1080556,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000693 DASZZC010000000
VERSION     DASZZC010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080556"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VHE37_14670"
     CDS             <1..650
                     /locus_tag="VHE37_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36831.1"
                     /translation="GHDYTLVEQADIHLALLAHLGVTRVDVLAHDYGVSVAQELLARE
                     LEGRTPRIDSVMLLNGGLFPEMHRARPIQKLLLSPLGPLLARFTGERSFSRSFSAIFG
                     PQTQPSAEELHAFWQLVTHNQGTRIFHRLIRYIPERRAQRERWVGALLRTRVPLRLVN
                     GPEDPVSGAHLAEHYRQHVPHPDIVSLPGIGHYPQVEAPAQVLAALAEFRARQPA"
     gene            740..2065
                     /locus_tag="VHE37_14675"
     CDS             740..2065
                     /locus_tag="VHE37_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT36832.1"
                     /translation="MGQDTVVEENAEHGGFPDRAPQTIGKYEVRGELGRGACGVVYKG
                     YDPFVARDVAIKVALQNPEMRAHASDRAFFVEARAAGMLQHPHIVATYDAGVEGDLSY
                     IVMEYIDGETLMPLCRQKGPRAPIDQVLDISFKCAKALDYAHGKGVLHRDIKPSNIML
                     TRDGVPKVMDFSIAEISGGNKDKDEGVLGSPLYMSPEQVRHQPLGPTSDLYSLGAVIY
                     QLLTGLPPFVETQLPVLFQQIRNVPAPRVELLRPETPRPLCDMVSRLLYKEPAQRFQS
                     GGELAESLTRLFDNLKLAEKQVSRRESKDSLRRLQFFSTFSDSEIDEIMNASSMLTYS
                     ENQGIIDEGDIDNAFYIIAVGTAEVRKGGKKLHTLNKGDCVGEIGFLTAAKRTASVVA
                     MTKVLALKINATLMDKVSRDCQLRFYKVFTETLIYRLTVTSAKLSAATA"
     gene            2074..2718
                     /locus_tag="VHE37_14680"
     CDS             2074..2718
                     /locus_tag="VHE37_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36833.1"
                     /translation="MNIAVRDGLKFHAVFFLTFALVLWLEHGSALGSGLLALAISYNV
                     LVPALGFWRGHAEWLSLWSFLAPLSMMQVLPDCALARIAHVLVFPDLAQYRIGGEVPV
                     YFMGLWIMLLFPILLLANAMRSRYTLAALLTLVLFAFCEWAARPLGLWHNEHVAMTFG
                     VAHYALLSELLLGLTALWMYRLTRHSNPLARAVGAICVSVFYTGSVFLALLLTG"
     gene            2782..4089
                     /gene="purD"
                     /locus_tag="VHE37_14685"
     CDS             2782..4089
                     /gene="purD"
                     /locus_tag="VHE37_14685"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HVT36834.1"
                     /translation="MKVLVIGSGGREHALAWKLAQSQRVSEVLVAPGNAGTAREPRCR
                     NVDVAAEDIAGLIALVRAEQVGFTVVGPEGPLAAGLVDAFNREGLRVFGPTRAAAQLE
                     ASKAFTKDFLARHRIPTAQYRVFTEPAPALAYVEQRGAPIVIKADGLAAGKGVVVAMT
                     LDEARAAVRDMLSGEMLGKAGARVVIEDFLDGEEASFIVVAGGTQYQALASAQDHKRI
                     GDRDQGPNTGGMGAYSPAPVVTPEVHARVCREIIEPTLAGMIAEGVPFTGFLYAGLMI
                     RKDGAPLVIEFNARMGDPETQPIMFRLESDLLDLLEAALDGRLASAAPRWHAQPAVGV
                     VMAAQGYPGTVKKGDAISGLDADFGEAKVFHAGTAMKGSEVVTAGGRVLCVCARGTSV
                     REAQQRAYAAVENIRWAGEQHRTDIGFRAIARENSQTATPMAP"
     gene            complement(4181..5137)
                     /gene="gcvA"
                     /locus_tag="VHE37_14690"
     CDS             complement(4181..5137)
                     /gene="gcvA"
                     /locus_tag="VHE37_14690"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HVT36835.1"
                     /translation="MQKRKLPPMDLLPAFEAAARHLSFTKAAGELFLTQSAISRQIQA
                     LEESLGVPLFQRRHRALLLTDAGQIFQRHVSEMLRQLTEATQEVRGDHAVRLLNVSTM
                     VSFASLWLVPRLPRFATAHPGVDVRLSANNDIVDLTRERIDVAIRYCLPHAAPPNAIK
                     LFGEVVMPMCSPTLVRDTGKPLNSPADLKHHILLHDEDASGRTPWLDWGNWLETQGLR
                     GLQPAGEVRFSHYDQMIQAAIGGHGVAIGRMPLLAGAMKRGQLIAPFDPKMLKAAPNS
                     ARVFYIVCEPRSASRPEVKAFVEWLSAETEVDEDLPRGRKRA"
     gene            5289..5516
                     /locus_tag="VHE37_14695"
     CDS             5289..5516
                     /locus_tag="VHE37_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36836.1"
                     /translation="MKTWLLTALILGALNTQPTLAPAPKADELPLAWQIAWQGDRALL
                     HIHRDNWRALRQIRPMPLEEVVRETTAVAAE"
     gene            complement(5569..6828)
                     /gene="rho"
                     /locus_tag="VHE37_14700"
     CDS             complement(5569..6828)
                     /gene="rho"
                     /locus_tag="VHE37_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HVT36837.1"
                     /translation="MHIAELKKKTAAELLQMAEELGLENVARTRKQDLVFNLLKAAAR
                     RGEHIYANGVLEIMQDGYGFLRGPDSSYLAGPDDVYISPSQIRRFSLRTGDTVAGRVR
                     SPKDNERYFALLKVDTINFEPLEVTKKKVSFENLTPLFADEQLKMERGNGTTEDITAR
                     VIDLVAPFGKGQRGLIVSPPKAGKTMMMQNIAQSIANNYPDVYLIILLVDERPEEVTD
                     MQRTVRAEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     VAPSSGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATALVDTGSKMDEVIYEE
                     FKGTGNMEIHLERRIAEKRVFPAININRSGTRKEELMMQAGDLTKVWILRKLLHDMDE
                     IAAMELLIDRMKQTKTNSQFFDSMKRN"
     gene            complement(7154..7483)
                     /gene="trxA"
                     /locus_tag="VHE37_14705"
     CDS             complement(7154..7483)
                     /gene="trxA"
                     /locus_tag="VHE37_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HVT36838.1"
                     /translation="MSQNISPVTDASFDQDVLKSSQPVLVDFWAEWCGPCRMIAPILE
                     QVAGENAGKLKVVKLNVDENTATPAKYGIRGIPTLILFKNGQVAATQVGAVHKAQLAA
                     FVTPHLA"
     gene            7735..9027
                     /locus_tag="VHE37_14710"
     CDS             7735..9027
                     /locus_tag="VHE37_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT36839.1"
                     /translation="MQPDHLSSIRFDQLPLHPALQDGLQRLGFSYCTPIQAQTLPRAL
                     AGEDLAGQAQTGTGKTAAFLLATMNHLLNHPRAPGSDMKQPRAFMLAPTRELAIQILK
                     DAESIGGNTGLKMAVCYGGAGYEVQRAAIEGGLDILIGTPGRLIDFYKQHAYDLKHIE
                     VLVLDEADRMFDLGFIADIRYLIRKMPPPAQRKNYLFSATLSQRVLELAYEHMNSPQK
                     IEIEPEKVTAERVRQSMIHVANDEKLGLLVGLLRHHDPKRTLVFVNMKVTAEQLERGL
                     KANGFEAATLSGDVAQSRRLRLLDDFKEGRLPILVATDVAARGLHIPEVSHVINYDLP
                     QDAEDYVHRIGRTARAGAVGDAISFCCETYVYSLPDIEKYIGHRIPQMEGGHELIAAD
                     AVQVERLRRPHGGRGHGGGRRGGGGGRGGHRGGGGRRR"
     gene            9065..>9627
                     /locus_tag="VHE37_14715"
     CDS             9065..>9627
                     /locus_tag="VHE37_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HVT36840.1"
                     /translation="MRTLLASLFAFFTLLPAAYADTYAEGKDYREARHAQPGTGKTIK
                     VEEYFWYGCPHCYAAEPYLESWLRTRPADVDFVRVPNTLGHDVGKQHAKAFYTADFLK
                     LLDQTHEAMFDAIHKEHRALNSPEAIQQFYTEKTGVPADKWNATFNGFAIDSRVRRAD
                     KDAMDYGISGTPTLVIDGRWLVDAKNPP"
BASE COUNT         1576 a         3178 c         3203 g         1670 t
ORIGIN      
        1 ccggccacga ttacacgctg gtcgagcagg ccgacatcca tctcgcgctg ctggcgcacc
       61 tgggcgtgac gcgcgtggac gtgctggcgc acgactacgg cgtgtcagtg gcgcaggaac
      121 tgctggcgcg cgaactggaa gggcgcacgc cgcggatcga ttcggtgatg ctgctcaacg
      181 gcggcctgtt tcccgagatg caccgcgcac ggccgatcca gaaactgctg ctgtcgccgc
      241 tgggtccgct gctggcgcgc ttcaccggcg agcgcagctt cagccggagc ttcagcgcca
      301 tcttcggtcc gcagacgcaa ccgtccgccg aagagctgca cgcattctgg caactggtca
      361 cgcacaacca gggcacgcgc atcttccacc ggctgatccg ctacatcccg gaacgccggg
      421 cgcagcgcga acgctgggtg ggcgcgctgc tgcgcacgcg cgtgccgctg cggctggtca
      481 acgggccgga agacccggtc tccggcgcgc acctggccga gcactaccgg cagcacgtgc
      541 cccacccgga catcgtttcc ctgcccggca tcggccacta cccgcaggtg gaggcgccgg
      601 cgcaggtgct ggcggcgctg gccgaattcc gggcacggca gccggcgtag cggggatttc
      661 cgctttcacc ccggcatggc gcggtttatg ctcgcgccag gtcgccgaag cattcgacac
      721 attactcaag gggaagtgcg tgggccagga caccgtcgtc gaggaaaatg ccgaacatgg
      781 cggctttccc gaccgcgcgc cccagaccat cggcaagtac gaagtgcgcg gcgaactggg
      841 ccgcggcgcc tgtggcgtgg tctacaaggg ctatgacccg ttcgtggcgc gcgacgtcgc
      901 catcaaggtg gcgctgcaga accccgagat gcgcgcccat gcctcggatc gggccttctt
      961 cgtcgaagcg cgtgccgccg gcatgctgca gcacccgcat atcgtcgcca cctatgacgc
     1021 cggcgtcgaa ggcgatctct catatatcgt catggagtac atcgacggcg aaacgctgat
     1081 gccgctgtgc cggcagaagg ggccgcgcgc gccgatcgac caggtgctcg acatcagctt
     1141 caagtgcgcc aaggcgctgg actacgcgca cggcaagggc gtgctgcacc gggacatcaa
     1201 gccgtcgaac atcatgctca cgcgcgacgg cgtgcccaag gtgatggact tctccatcgc
     1261 cgagatctcc ggcggcaaca aggacaagga cgagggcgtg ctcggttcac cgctgtacat
     1321 gtcgcccgaa caggtgcggc accagccgct agggccgact tccgacctgt attcgctcgg
     1381 ggcggtgatc taccagctgc tcaccgggct gccgccgttc gtggagacgc agctgccggt
     1441 gctgttccag cagatccgca acgtgccggc gccgcgcgtg gagctgctgc gcccggaaac
     1501 gccgcgcccg ctgtgcgaca tggtgtcgcg cctgctgtac aaggagccgg cgcagcgctt
     1561 ccagtcgggc ggcgaactgg ccgaatcgct gacccggctg ttcgacaatc tcaagctggc
     1621 ggaaaagcag gtgtcgcgcc gcgaatccaa ggattcgctg cgccggctgc agttcttcag
     1681 caccttctcc gacagcgaga tcgacgagat catgaacgcg tcgagcatgc tcacctactc
     1741 ggagaaccag ggcatcatcg acgagggcga catcgacaac gccttctaca tcatcgccgt
     1801 cggcaccgcc gaggtgcgca agggcggcaa gaagctgcac acgctgaaca agggcgactg
     1861 cgtcggcgag atcggatttc tcacggcggc caagcgcacc gccagcgtgg tggcgatgac
     1921 caaggtgctg gcgctgaaga tcaacgccac gctgatggac aaggtttcgc gcgactgcca
     1981 gctgcgtttt tacaaggtgt tcaccgagac actgatctac cgcctgaccg tgaccagcgc
     2041 aaaactttcc gccgccaccg catgagggac cagatgaaca ttgcggtcag ggacggcctc
     2101 aagtttcatg cggtcttctt cctcaccttc gcgctggtgc tgtggctgga gcatggctcg
     2161 gcgctgggca gcggcctgct ggcgctggcc atcagctaca acgtgctggt gccggcgctg
     2221 ggcttctggc gcggccacgc cgagtggctg tcgctgtggt cgttcctggc gccgctgtcg
     2281 atgatgcagg tgctgccgga ttgcgcgctg gcgcgcatcg cccacgtgct ggtattcccg
     2341 gacctggcgc agtaccgcat cggcggcgag gtgccggtct atttcatggg cctgtggatc
     2401 atgctgctgt tcccgatcct gttgctggcc aatgccatgc gcagccgcta cacgctggcg
     2461 gcgctgctca cgctggtgct gttcgccttc tgcgagtggg ccgcgcggcc gctgggcctg
     2521 tggcacaacg agcacgtggc catgaccttc ggcgtggcgc actacgcgct gctgtccgaa
     2581 ttgctgcttg ggctcaccgc gctgtggatg taccggctca cgcgccacag caatccgctg
     2641 gcgcgcgcgg tgggcgccat ctgcgtctcg gtgttctaca ccggctcggt gttcctcgcg
     2701 ctgctgctga ccggctgagg ttcctgccgc gcacgcggca atgacaaaat ggcgccccgc
     2761 aaaaaaccac gggcgtatcc catgaaggtt ctggtcatcg gcagcggcgg gcgcgaacac
     2821 gcgctggcct ggaagctggc gcagtcgcaa cgcgtgtccg aggtgctggt cgcgccgggc
     2881 aacgccggca ccgcgcgcga gccacgctgc cgcaacgtcg acgtcgcggc cgaggacatc
     2941 gccgggctga tcgcgctggt gcgtgccgag caggtcggct tcaccgtggt cgggccggaa
     3001 ggtccgctgg ccgccggact ggtcgatgcc ttcaaccgcg aagggctgcg cgtgttcggc
     3061 cccacccgcg ccgccgcgca gctcgaggcg agcaaggcat tcaccaagga tttcctcgcg
     3121 cggcaccgga ttcccaccgc gcagtaccga gtgttcacgg aacctgcgcc cgccttggct
     3181 tacgtggagc agcgcggcgc gcccatcgtg atcaaggccg atggcctggc cgccggcaag
     3241 ggcgtggtgg tggcgatgac gctggacgag gcgcgcgccg ccgtgcgcga catgctgtcc
     3301 ggcgagatgt tgggcaaggc cggcgcgcgc gtggtgatcg aggattttct tgacggcgag
     3361 gaggccagct tcatcgtcgt cgccggcggc acgcagtacc aggcgctggc cagtgcgcag
     3421 gatcacaagc gcatcggcga tcgtgaccag ggccccaaca ccggcggcat gggcgcctac
     3481 tcgccagcgc cggtggtcac gcccgaggtg catgcacgcg tgtgccgcga gatcatcgag
     3541 cctactcttg ccggcatgat cgccgagggc gtgcccttca ctggcttcct ctatgccggc
     3601 ctgatgatcc gcaaggacgg cgcgccgctg gtgatcgaat tcaacgcgcg catgggcgat
     3661 ccggaaacgc agccaatcat gttccgcctg gaatccgacc tgctggacct gctggaggcc
     3721 gcgctcgacg gcaggctggc atcggcggcg ccgcgctggc acgcgcagcc tgcggtcggc
     3781 gtggtgatgg cggcgcaggg ttatcccggc acggtgaaga aaggcgatgc catttccggc
     3841 ctcgatgctg atttcggcga ggccaaggtg ttccacgcgg gcaccgcgat gaagggcagc
     3901 gaggtcgtca ccgccggcgg gcgcgtgctg tgcgtgtgcg cgcgcggcac cagcgtgcgg
     3961 gaagcgcagc agcgcgccta tgccgcggtg gagaacatca gatgggccgg cgagcagcat
     4021 cgaaccgaca tcggttttcg cgcgatcgcg cgcgaaaaca gccagacagc gacgccaatg
     4081 gcgccgtaag tcagcatcct ctctccccgc tggcgggagg agattggagt gaggggcgcc
     4141 cgcgcaagcg ggccttcgcg cgatcgcgcg cgaaagctga tcaggcgcgc ttgcgcccgc
     4201 gcggcaggtc ttcgtcgact tcggtctcgg cgctgagcca ctcgacgaag gccttcactt
     4261 ccggccggga agccgaacgc ggctcgcaca cgatgtagaa cacgcgcgcg gagttgggcg
     4321 ccgccttcag catcttcgga tcgaacggcg cgatcagctg cccgcgcttc atcgcaccgg
     4381 ccagcagcgg catgcggccg atggccacac cgtggccgcc gatggccgcc tggatcatct
     4441 ggtcgtagtg cgagaagcgc acttcgccgg ccggctgcag cccgcgcagg ccctgcgtct
     4501 ccagccagtt gccccagtcc agccacggcg tgcggcccga cgcgtcttcg tcgtgcagca
     4561 ggatgtggtg cttgaggtcg gccggcgaat tgagcggctt cccggtgtcg cgcaccagcg
     4621 tcggactgca catcggcatc accacttcgc cgaacagctt gatggcattg ggcggcgctg
     4681 cgtgcggcag gcagtagcgg atggccacgt cgatgcgctc gcgcgtgaga tcgacgatgt
     4741 cgttgttggc cgacagccgc acgtcgacgc ccggatgcgc cgtggcgaag cgcggcagcc
     4801 gcggcaccag ccacagcgag gcgaacgaca ccatggtgct cacgttgagc agccgcaccg
     4861 cgtggtcgcc gcgcacttcc tgcgtggcct cggtcagctg gcgcagcatc tcgctgacgt
     4921 gccgctggaa gatctggccc gcatcggtca gcagcagcgc gcggtgcctg cgctggaaca
     4981 gcggcacccc gagcgattcc tccagtgcct ggatctggcg gctgatggcc gactgcgtca
     5041 ggaacagttc gcccgcggcc ttggtgaacg aaagatgccg cgccgcggct tcgaaggcgg
     5101 gcaacaggtc catcggtggc agcttgcgtt tttgcattct atttactcat gcgtgcaatg
     5161 agaacttacc gtttgaggca gagcccactc atcgtcacaa tgctctccgt ggcaagcagg
     5221 aaacggatca agagcaagcc cgctgcatac cgttcctgca tgcaagatta gcatggagac
     5281 tcaccgccat gaagacctgg ctcctgaccg cgctgatcct cggcgccctc aatacccaac
     5341 ccaccctggc gcctgcgccg aaggccgatg agctgccgct ggcctggcag attgcctggc
     5401 aaggcgaccg cgcgctgctg catatccacc gcgacaactg gcgcgcgctg cgccagatca
     5461 ggcccatgcc gctggaagag gtggtgcgtg aaactaccgc ggtcgccgcc gagtagacac
     5521 gaataaaaaa gccccggatg gccggggctt ttctgcacat caaagccgct agttcctctt
     5581 catcgaatcg aagaactgcg agttggtctt ggtctgcttc atgcggtcga tcagcagctc
     5641 catggcggcg atctcgtcca tgtcgtgcag cagcttgcgc aggatccaga ccttggtcag
     5701 atcgccggcc tgcatcatca gctcttcctt gcgcgtgccg gagcggttga tgttgatggc
     5761 cgggaacacg cgcttctcgg cgatgcggcg ctccaggtgg atttccatgt tgccggtgcc
     5821 cttgaactcc tcgtagatca cctcgtccat cttgctgccg gtgtcgacca gcgcggtggc
     5881 gatgatggtc agcgagccgc cttcctcgat gttgcgcgct gcgccgaaga agcgcttggg
     5941 cttctgcagg gcattggcgt cgacaccgcc ggtgagcacc ttgcccgacg aaggcgccac
     6001 cgtgttgtaa gcgcgcgcca ggcgggtgat ggaatccagc aggatgacca cgtcacgctt
     6061 gtgctcgacc aggcgcttgg ccttctcgat caccatttcc gccacctgca cgtggcgcgt
     6121 ggccggctcg tcgaaggtcg agctgatgac ctcggcgcgc acggtgcgct gcatgtcggt
     6181 gacttcttcc gggcgctcgt cgaccagcag gatgatcagg tagacgtccg ggtaattgtt
     6241 ggcaatggac tgcgcgatgt tctgcatcat catcgtcttg ccggccttgg gcggcgacac
     6301 gatgaggccg cgctggccct tgccgaacgg cgccaccaga tcgatcacgc gcgcggtgat
     6361 gtcctcggtg gtgccattgc cgcgctccat cttcagctgc tcgtcggcga acagcggcgt
     6421 caggttctcg aacgagacct tcttcttggt gacttcgagc ggctcgaagt tgatggtgtc
     6481 gactttcagc agcgcgaagt aacgctcgtt gtccttggga ctgcgcacgc gcccggccac
     6541 ggtatcgccg gtacgcagcg agaagcggcg gatctgcgag ggcgagatat agacgtcgtc
     6601 cgggccggcg agatacgaac tgtccggccc gcgcaggaaa ccgtagccgt cctgcatgat
     6661 ctcgagcacg ccgttggcgt agatatgctc gccgcggcgc gcggcggcct tgagcaggtt
     6721 gaacaccagg tcctgcttgc gcgtgcgggc cacgttttcc aggcccagct cttccgccat
     6781 ctgcagcagt tcggcggcgg ttttcttctt cagttcggcg atgtgcagga ccttggccgg
     6841 ctcggcgggc tgctgggacg agcccggcgc ctggccgttg accgcattgg catcttcctc
     6901 gccgtcctcg cccatctgcg gggcggggcc gccgcgcgtg gcctgcgcga tggcggcatc
     6961 gtcgccgtcg gcgtattccg ggttttccga actcgccggc tgcaggtcga cttcgtaatc
     7021 gcggtcgttg cgctggcggt aattgccgtc gcgctggttg ccgttgtagt tcccgttccc
     7081 gttgccgtga ttcgggttgt tctgccgctg gccgccgtga cgacgacgat gaggaccttg
     7141 gcgcatgtat gccctatgcg agatgcggcg tgacgaaggc cgcgagttgt gccttgtgca
     7201 cggcgcccac ctgggtcgcc gccacttggc cgttcttgaa cagaatgagg gtcgggatgc
     7261 cgcggatgcc gtacttggcc ggcgtggcgg tgttctcgtc gacgttgagc ttgaccacct
     7321 tgagcttgcc ggcattctcg ccggccacct gctccaggat cggcgcgatc atgcggcacg
     7381 gcccgcacca ctcggcccag aagtcgacca gcaccggctg gctggacttg agcacgtcct
     7441 ggtcgaagga ggcgtcggtg acggggctga tgttctggct catggttgga cctcgtgtgg
     7501 gagactcgcg cgaatggtgc gcgtctgcag cgatatgacc gtttgcgcct ttttcaagcg
     7561 ctgcccgggg cgcgcatgaa aaaaggctgg ttgttatgat tagaagttct aggggatcga
     7621 acgagcggct gtcgcagcgg gcgtccaaaa acgcgtcagg tgcggccggg atggacggta
     7681 tttgagccta actcccgccg cattgcaaga cccgaccctc acctgacaag ttccatgcaa
     7741 cctgaccatc tctccagcat ccgtttcgac cagttgccgt tgcatcctgc attgcaggac
     7801 ggcctgcagc ggctcggctt ttcctactgc acgccgatcc aggcgcagac gctgccgcgc
     7861 gcgctggccg gcgaagatct cgccggccag gcgcagaccg gcaccggcaa gaccgccgcc
     7921 ttcctgctgg cgacgatgaa ccatctgctc aatcatccgc gcgcgcccgg cagcgatatg
     7981 aagcagccgc gcgcgttcat gctcgccccc acgcgcgaac tggccatcca gatcctcaag
     8041 gacgccgagt ccatcggcgg caacaccggg ctgaagatgg cggtgtgcta tggcggcgcc
     8101 ggctacgagg tgcagcgcgc cgccatcgag ggcgggctcg acatcctgat cggcacgccg
     8161 ggacgcctga tcgacttcta caagcagcat gcctacgacc tcaagcacat cgaggtgctg
     8221 gtgctggacg aggcggaccg catgttcgac ctcggcttca tcgccgacat tcgctatctc
     8281 atccgcaaga tgccgccgcc ggcgcagcgc aagaactacc tgttctccgc cacgctctcg
     8341 cagcgcgtgc tcgaactcgc ctacgagcac atgaacagcc cgcagaagat cgagatcgag
     8401 ccggagaagg tcaccgccga gcgcgtgcgc cagtcgatga tccacgtggc caacgacgaa
     8461 aaactgggcc tcttggtcgg cctgctgcgc caccacgatc ccaagcgcac gctggtgttc
     8521 gtcaacatga aggtcaccgc cgagcagctg gagcgcgggc tcaaggcgaa tggattcgag
     8581 gccgccacgc tgtcggggga cgtggcgcag tcgcgccgcc tgcgcctgct cgacgacttc
     8641 aaggaaggcc gcctgccgat cctggtggcc accgacgtgg ccgcgcgcgg gctgcacatt
     8701 cccgaggtgt cgcacgtgat caactacgac ctgccgcagg acgcggaaga ctacgtgcac
     8761 cgcatcggcc gcaccgcgcg cgccggcgcc gtgggcgacg ccatctcgtt ctgctgcgag
     8821 acctacgtgt actcgctgcc ggacattgag aaatacatcg gccatcgcat cccgcagatg
     8881 gaaggcggcc atgaactgat tgccgccgac gctgtccagg tggagcgcct gcgccgtccg
     8941 catggcggcc gcggtcatgg cggcggtcgc cggggtggtg gcggcggccg tggcgggcat
     9001 cgcggcggcg gcggtcgtcg tcgctgatca ttttttaccc aaccggactt aagggaattt
     9061 ccacgtgcgc accttgcttg ccagcctgtt cgccttcttc acgctgctcc cggccgccta
     9121 tgccgacacc tacgcggaag gcaaggacta ccgcgaggcg cgtcatgcgc agccgggcac
     9181 cggcaagacc atcaaggtcg aggaatattt ctggtacggc tgtccgcatt gctacgcggc
     9241 cgagccctac ttggaatcct ggctgcgcac cagaccggcc gatgtcgatt tcgtgcgcgt
     9301 gcccaacacg ctgggccacg acgtgggcaa gcagcacgcc aaggctttct acaccgcgga
     9361 tttcctcaag ctgctggacc agacgcacga agcgatgttc gacgccatcc acaaggagca
     9421 ccgcgcgctc aactcgccgg aagcgatcca gcagttctac accgagaaga ccggcgtccc
     9481 ggcggacaag tggaacgcta cgttcaacgg ctttgccatc gactcgcgcg tgcgccgtgc
     9541 ggacaaggac gcgatggact atgggatcag cggcacgccg acgctggtca tcgacggccg
     9601 ctggctggtc gatgccaaga acccgcc
//
LOCUS       DASZZC010000694         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1080665,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000694 DASZZC010000000
VERSION     DASZZC010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080665"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="zapE"
                     /locus_tag="VHE37_14720"
     CDS             <1..843
                     /gene="zapE"
                     /locus_tag="VHE37_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HVT36841.1"
                     /translation="LPFTRKLRTHFHRFMMDVHERRKHYPNVEDPLLKVADEYAAKTR
                     VICFDEFFVSDIADAMILGRLFEALFARGITLVATSNIPPDELYRNGLQRANFLPAIE
                     QLKQNVSVLNVDGGTDYRLRALTHAEIYHWPCDAAAEKNLETWFSQMAHDSGRRNVDL
                     LVLERPIRARRLADGIAWFDFAALCEGPRSANDYVELARTYHTLLLSKVPQLGVSGED
                     PARRFINLVDELYDRGVKLIVTADVPVEQLYAGEKLKFEFQRTRSRLAEMQSQEYLAR
                     AHLA"
     gene            944..>1841
                     /locus_tag="VHE37_14725"
     CDS             944..>1841
                     /locus_tag="VHE37_14725"
                     /inference="COORDINATES: protein motif:HMM:NF041895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HVT36842.1"
                     /translation="MIARAGCCALACACAGWTLSAGAYSSGISGYTQGLACMGCHNNG
                     VGTTIPATGFSGPDVVVTGSSNSYSFGISAAGNGAVGGLDVSASAGTLATVAGSNTEL
                     LSGEITHTAPRSYIGAATSWTFTLTAPAAEGSLTLAGCGLNANHNGTDTGDAGACTAK
                     TITVKAPPVAGDDSYLVTEDSGTSNLAVLANDSDSSAIRVTAVNGSSSFPVLTAHGGS
                     LALSGPGAGNQLLYTPAADFSGTDTFTYQVTDTYSISSSGQVTVTVSAVNDAPRITAP
                     PPDRAVEGLPYSAQLPVTDPDDA"
BASE COUNT          329 a          666 c          569 g          277 t
ORIGIN      
        1 ctgccattca cgcgcaagct gcgcacgcat ttccaccgtt tcatgatgga cgtgcacgag
       61 cggcgcaagc actaccccaa cgtcgaagac ccgctgctca aggtggcgga cgaatacgcg
      121 gcgaagacgc gggtgatctg cttcgacgag ttcttcgtct ccgacatcgc cgacgccatg
      181 atcctcggcc gcctgttcga ggcgctgttc gcgcgcggca tcacgctggt ggccaccagc
      241 aacatcccgc ccgacgagct gtaccgcaac ggcctgcagc gcgccaactt cctgccggcc
      301 atcgagcagc tgaagcagaa cgtgtccgtg ctcaacgtcg acggcggcac cgactaccgc
      361 ctgcgcgcgc tgacccatgc cgagatctac cactggccat gcgacgccgc cgcggagaaa
      421 aacctggaga cctggttctc gcagatggcg cacgacagcg gcaggcgcaa cgtcgacctg
      481 ctggtgctgg aacgcccgat ccgcgcgcgg cgcctggccg acggcatcgc ctggttcgac
      541 ttcgccgcgc tgtgcgaggg cccgcgcagc gccaacgatt acgtggaact ggcgcgcacc
      601 tatcacacgc tgctgctgtc gaaggtgccg cagctgggcg tgtccggcga agacccggcg
      661 cggcgcttca tcaacctggt cgacgaactc tacgaccgcg gtgtcaagct catcgtcacc
      721 gccgacgtgc cggtggaaca gctttacgcc ggcgagaaac tcaagttcga attccagcgc
      781 acccgctcgc gcctggccga gatgcaatcg caggaatacc tggcgcgggc gcacctggcc
      841 tgagccgcgc aacgggcacg ggttccttta cagccgcagg cgttcgggct ataccgtccg
      901 cggccgcgcc aaggaaggaa ccagggcgcc gtcgggagga cttgtgatcg ccagagcagg
      961 atgctgcgcc cttgcatgcg cttgcgcggg atggacgctt tccgccggcg cctattccag
     1021 cggcatcagc ggctacacgc agggcctcgc ctgcatgggc tgccacaaca acggtgttgg
     1081 cacgaccatt ccggccacgg gattcagcgg tccggacgtg gtagtcaccg gctcctccaa
     1141 cagctacagc tttggcatca gcgccgccgg caatggcgct gtcggcggcc tcgacgtgtc
     1201 cgcgagcgcc ggcacgctgg caacggtcgc cggcagcaat acggaactgc tctccggcga
     1261 gattacgcat acggcgccgc gctcttacat cggcgctgcc accagctgga cttttacgct
     1321 caccgcaccc gccgccgaag gctcgctgac gcttgccggc tgcggcctca atgccaacca
     1381 caacggcacc gacaccggcg acgccggtgc ctgcaccgcc aagacgatca cggtgaaagc
     1441 gccgccggtg gcaggagacg acagttatct ggtcaccgag gacagcggca ccagcaatct
     1501 cgccgtgctg gccaacgaca gcgacagcag cgccatccgc gtcaccgcgg tgaatggctc
     1561 cagctcattc ccggtgctca ccgcccatgg cggcagcctt gcgctgtccg gccctggcgc
     1621 cggcaaccag ctgctctaca caccggcggc ggatttctcc ggcaccgaca ccttcaccta
     1681 ccaggtcacc gacacgtact ccatctcctc cagcgggcag gtgacagtca cggtgagcgc
     1741 ggtcaacgac gcgccgcgca tcacggcgcc gccgccggac cgtgccgtcg aaggactgcc
     1801 ttacagcgcg cagttgccgg taaccgatcc cgacgatgcc a
//
LOCUS       DASZZC010000695         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1210544,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000695 DASZZC010000000
VERSION     DASZZC010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210544"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VHE37_14730"
     CDS             <1..1002
                     /locus_tag="VHE37_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HVT36843.1"
                     /translation="EMLETLIRRAGSKLRSEVICQATLRGQYLPVHCVELGSTSPAVP
                     AVGFFGGVHGVERIGTQVLLAFMHALIERLNWDDVLAQILDQVRLVFVPLVNPGGMYN
                     RTRANPAGVDLMRNAPVEAEHAAFLLGGQRISSTLPWYRGRAGAPMEPEARAVCEVVQ
                     QRLHTHAFSLSLDCHSGFGSIDRIWFPYAKTRRPVECLAEIYALRSMFRSTHPYHSIY
                     VIEPQSYQYTTHGDLWDHLYDQSLADPSRLFIPLTLEMGSWLWVKKSPSQIFNLLGVF
                     NPVAPHRHQRALRRHLTFLDFLIRAAISHNRWRPPPQARAALLQSAQAYWYSGRQTM"
     gene            complement(1004..1480)
                     /locus_tag="VHE37_14735"
     CDS             complement(1004..1480)
                     /locus_tag="VHE37_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVT36844.1"
                     /translation="MVRLDALDVRILDLLQQDATLPIADVAQKAGSSKTVVWRRMQKY
                     LKSGVIRERVAVLDAAKVGLGIMIIAQVKMSRHGKDVLPNFLKAVKLFPQVIECHTLM
                     GDVDFLLKIMCKSVEDYEHFFWQKLSKIDGVQGISSSISMSRTVDTTRLPLARSDS"
     gene            1559..2827
                     /locus_tag="VHE37_14740"
     CDS             1559..2827
                     /locus_tag="VHE37_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVT36845.1"
                     /translation="MAKSKNIGSAAKKPEERMTLKLHIPEPKARPGEAACFDHLQIPK
                     AGLLPRPAVDAPEHELRDFPYGLIRVLDDQHKAVGAWNPKLDTEMLLRGLKDMQKTRV
                     FDDRLVKLQRQGKTSFYIKSTGEEAVAIAQGYALDKNDMLFPSYRVQGLLFARNWPMV
                     DMICQVFNNSRDRLKGRQLPIMYSVKEVGFFSISGNLGTQFPQAVGWAMASALKGDTR
                     IAASWVGDGTTAEADFHYALTFASVYNVPVLLNVVNNQWAISSFQGFAGGERTTFAAR
                     ATGYGLPGLRVDGNDFLAVYAATKWAVDRARANLGATVIELYTYRAAAHSTSDDPSAY
                     RPAEEARRWPLGDPIERLKQHLIGLGAWSEEQHDALVKELDEEVKAAIKEAEQYGTLA
                     DPHIHPGSMFEDVYKDIPEMLKLQMKEAGV"
     gene            2830..>3218
                     /locus_tag="VHE37_14745"
     CDS             2830..>3218
                     /locus_tag="VHE37_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HVT36846.1"
                     /translation="MAQMNMIQAINSAMDVMMARDSNVVVFGEDVGYFGGVFRCTAGL
                     QKKYGKTRCFDSPIAEGGIIATAVGMGVYGLRPVAEIQFADYIYPAFDQLVSEAARLR
                     YRSGGEYFAPITVRTPCGGGIYGGQTH"
BASE COUNT          570 a         1061 c         1046 g          541 t
ORIGIN      
        1 gaaatgctgg agacgctgat ccgccgcgcc ggcagcaagc tgcgcagcga ggtgatctgc
       61 caggccacgc tgcgcggaca gtacctgccg gtgcattgtg tggagctggg cagcacctcg
      121 ccggcggtgc cggcggtggg cttcttcggc ggcgtgcacg gcgtcgagcg catcggcacg
      181 caggtgctgc tggccttcat gcacgcgctg atcgagcggc tcaactggga cgatgtgctg
      241 gcgcagatcc tcgaccaggt gcgcctggta ttcgtgccgc tggtcaatcc cggcggcatg
      301 tacaaccgca cgcgcgccaa tccggccggc gtcgacctga tgcgcaacgc gccggtggaa
      361 gccgagcacg cggccttcct gctcggcggc cagcgcatct cctcgacgct gccctggtac
      421 cgcggccgcg ccggcgcgcc gatggagccg gaggcgcgcg cggtgtgcga ggtggtgcag
      481 cagcgcctgc acacgcacgc cttctcgctt tcattggact gccattccgg tttcggcagt
      541 atcgaccgca tctggtttcc ctacgccaag acgcggcggc cggtggaatg cctggccgag
      601 atctacgcgc tgcgctcgat gttccgcagc acccatccgt atcacagcat ctacgtgatc
      661 gagccgcagt cgtaccagta caccacgcac ggcgacctgt gggatcacct ctacgaccag
      721 tcgctggccg acccgtcgcg cctgttcatc ccgctgacgc tggagatggg cagctggctg
      781 tgggtgaaga agagcccgtc gcagatcttc aacctgctgg gcgtgttcaa cccggtggcg
      841 ccgcaccgcc accagcgcgc gctgcgccgg cacctgacct tcctcgattt cctgatccgc
      901 gcggccatct cgcacaaccg ctggcgcccg ccgccgcagg cgcgcgcggc gctgctgcag
      961 tcggcccagg cctactggta cagcggcagg cagaccatgt agttcaactg tcgctgcgcg
     1021 ccagcggcag ccgcgtagtg tccaccgtgc ggctcatgga gatggacgag ctgatgccct
     1081 gcacgccgtc tatcttcgac agcttctgcc agaaaaaatg ctcgtagtct tccacgctct
     1141 tgcacatgat cttgagcagg aagtcgacgt cgcccatcag cgtgtggcac tcgatcacct
     1201 gcggaaagag tttcaccgcc ttgaggaagt ttggcagcac gtccttgccg tgccgcgaca
     1261 tcttcacctg cgcgatgatc atgatgccca ggccgacctt ggccgcgtcg agcaccgcca
     1321 cgcgctcgcg gatcacgccg ctcttcaggt atttctgcat gcgtcgccag accaccgttt
     1381 tggatgagcc ggctttttgc gcgacgtcgg cgatcggcag ggtggcgtcc tgctgcagca
     1441 ggtcgaggat gcggacatcg agtgcgtcga ggcggaccat tgttccgggt atctggcaaa
     1501 tccgggaaca gagtttcaca atggaccgca aggcgcaaca gaaaaggacg aaattccgat
     1561 ggcgaagtcc aagaatatcg gcagcgcggc gaaaaagccg gaggagcgca tgaccctgaa
     1621 gttgcacatc ccggagccca aggcccgtcc gggcgaggcc gcctgtttcg atcacctgca
     1681 gattcccaag gcggggctgc tgccgcggcc ggcagtggac gcgccggaac acgagctgcg
     1741 tgattttccc tacggcctga tccgcgtgct ggacgaccag cacaaggcgg tgggcgcgtg
     1801 gaacccgaag ctcgacaccg agatgctgct gcgcggcctg aaggacatgc agaagacgcg
     1861 cgtgttcgac gaccgcctgg tgaaactgca gcgccagggc aagaccagct tctacatcaa
     1921 gagcaccggc gaggaagcgg tggccatcgc ccagggctat gcgctggaca agaacgacat
     1981 gctgttcccg tcgtaccgcg tgcaagggct gctgttcgcg cgcaactggc cgatggtcga
     2041 catgatctgc caggtattca acaactcgcg cgaccgccta aagggccgcc agctgccgat
     2101 catgtactcg gtgaaggaag tgggcttctt ctccatctcc ggcaacctcg gcacgcagtt
     2161 tccgcaggcg gtgggctggg ccatggcctc cgcgctcaag ggtgacacgc gcatcgccgc
     2221 cagctgggtg ggcgacggca ccacggccga ggccgatttc cactacgcgc tgacctttgc
     2281 ctcggtctac aacgtgccgg tgctgctcaa cgtggtgaac aaccagtggg ccatctcgtc
     2341 gttccagggt ttcgccggcg gcgagcgcac cacctttgcg gcgcgcgcca cgggctatgg
     2401 attgcccggc ctgcgcgtcg acggcaatga tttcctcgcg gtgtatgccg ccaccaagtg
     2461 ggcggtggac cgcgcgcgcg ccaatctcgg cgccaccgtg atcgagctgt acacctaccg
     2521 cgccgccgcg cattccacca gcgacgatcc gagcgcttat cgtcccgccg aagaggccag
     2581 acgctggccg ctgggcgacc cgatcgagcg gctcaagcag cacctgatcg gcctgggcgc
     2641 ctggagcgaa gagcagcacg acgcgctggt caaggaactc gatgaagagg tcaaggccgc
     2701 gatcaaggaa gccgagcagt acggcacgct ggccgatccg cacatccacc cgggttcgat
     2761 gttcgaggac gtctacaagg acatacccga gatgctgaag ctgcagatga aggaagcggg
     2821 agtctgatca tggcccagat gaacatgatc caggccatca actccgccat ggatgtgatg
     2881 atggcgcgcg acagcaacgt ggtggtgttc ggcgaagacg tcggctactt cggcggcgtg
     2941 ttccgctgca ccgccggcct gcagaagaaa tacggcaaga cgcgctgctt cgattcgccg
     3001 attgccgagg gcggcatcat cgccaccgcc gtcggcatgg gcgtctacgg cctgcgcccg
     3061 gtggcggaga tccagttcgc cgactacatc tatcccgcat tcgaccagct ggtgtcggaa
     3121 gccgcgcgcc tgcgctaccg ctcgggcggc gaatactttg cgccgatcac cgtgcgcacg
     3181 ccctgcggcg gcggcatcta cggcgggcag acgcacag
//
LOCUS       DASZZC010000696         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_962616,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000696 DASZZC010000000
VERSION     DASZZC010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962616"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VHE37_14750"
     CDS             complement(<1..1026)
                     /locus_tag="VHE37_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HVT36847.1"
                     /translation="MQTQASCAQRARIWLRAAIAIAALVVSPLSPAANGDPYMQTILH
                     FFPQAEEAYLTGSLLKARDGNLYGISAMGGASGGGFIYMVTPDGTYSVIHDFVANDGQ
                     SPSVGLMEGSDGYFYGITDQGGPLGHGTIYRFAPSGYFQLLHAFTAHEPNVGAGPLIE
                     GADGRVYGTLQYGPSDVGASGAVFSMSMNGDDFVVMHAFAGPYDAGWPMSGLTIGPDG
                     NFYGATHWRPGGDTHDVTGGSIYQMTPSGDVTLLYSFASDFSEGIEPSGVIFGSDGNL
                     YGTTGEGGANWSGTIFRLTLQGEMTTLHAFAQGDGLPRAALIAGADGLMYGSTDAYYQ
                     SPFVYSLA"
     gene            complement(1370..>3197)
                     /locus_tag="VHE37_14755"
     CDS             complement(1370..>3197)
                     /locus_tag="VHE37_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT36848.1"
                     /translation="HGAREGLRHARDELTRANASLTQRVDQLHAAEEKLSGILESIDN
                     IVWSVNALSHEMQYVSAAVERVHGRPVSEFQSDPLLWLKVIHVDDRASVRHWLAQLVE
                     QGAATLQYRIVRPGGEVRWLEDRARLVRDAQGQPLRLDGVASDISERKSHEAEMSYLA
                     NHDDLTGLPNRNLLNDRVAQALAQARRTGHSLAVMFMDLDGFKFINDSFGHNLGDALL
                     RSVAGRLTQAVREGDTVARLGGDEFVIMLWDIAARDAISAIAGKLLAALSQPLSADGR
                     ELHVSASIGISVYPQDGENFETLLKHADVAMYRAKELGRSGYKFYTQEMSAQTDERVE
                     MEAALRQALARDELSLHYQPQVDIKTGGIFGVEALIRWQHPKFGAVPPGRFIRLAEDT
                     GLILPIGEWALRSACAQLQAWRDAGHAALSVAVNISARQFHQQDVPALVRSVCRDTGL
                     PPSRLELELTESTLINNSDAVIETLRELEDMGVVLAMDDFGTGYSSLSYLKRFPIDVI
                     KIDQSFTADVCSDADAASITRAIIAMARSLSIKTVAEGVENAEQLEFMRRTGCDAIQG
                     HWFSRALTAVELGELLDDVRAVSAADYAAIWSSRRMRPPLAA"
BASE COUNT          555 a         1059 c         1006 g          577 t
ORIGIN      
        1 cgcaaggctg taaacgaacg gagattgata gtaggcatcg gtcgagccgt acatcagccc
       61 gtctgccccg gcgatgagcg cggcgcgcgg caagccgtcg ccctgggcaa aggcatggag
      121 cgtggtcatt tctccctgca gggtgaggcg gaaaatcgtt ccgctccagt tcgctccgcc
      181 ctctccggtc gtgccgtaga gattgccatc gctgccgaaa atcacgcccg atggttcaat
      241 gccctcggaa aaatcgctgg caaagctgta cagcagcgtg acgtcgcccg aaggcgtcat
      301 ctggtagatg ctgccaccgg tgacgtcgtg cgtatctcca cccgggcgcc aatgcgtcgc
      361 accgtagaag ttgccgtcgg gaccgatggt cagtccactc attggccaac ctgcatcgta
      421 aggaccggcg aacgcatgca tgacgacgaa atcgtctcca ttcatgctca tgctgaacac
      481 cgctccggaa gcgcccacat cactggggcc gtattgcaga gtcccataga cccgtccgtc
      541 ggcgccttcg atcaacggac ctgcgcctac gttcggctca tgggcagtga aggcgtgcag
      601 caattggaag taacccgaag gcgcaaagcg gtagatggtt ccatgaccca acggcccccc
      661 ctggtcggtg atgccataga aatagccgtc gctgccctcc atcagcccga cgcttgggct
      721 ctgaccgtcg ttggcgacga agtcatggat caccgaatag gttccatccg gggtcaccat
      781 gtaaataaat ccgccaccgg acgcaccgcc catggcactg atgccatata gattgccgtc
      841 acgagccttg agcagagagc ccgtcaggta ggcctcctct gcctgcggga aaaaatgcag
      901 gatggtctgc atatacggat cgccattggc cgctggcgaa agcgggctca cgaccagtgc
      961 cgcaatcgca atggccgcac gcaaccagat gcgcgccctt tgggcgcaac ttgcctgagt
     1021 ctgcattgat gttcccccca acatggcctg ctccccaacc accggcgcgg ctgtcccttc
     1081 agccgcacct ggcgcgaaac tttttttgcg ccgaccgcgt cctgcgcatc ggcgaatcgc
     1141 ttgttaagtc gcgcggcccg gagtgcccgg gccggactca tttgaaccga cgttttggaa
     1201 aggcgatgta ggcggcagac gcggggcggc gtcggccaat tcctacgtag gctgccatgc
     1261 cccctccacc gcacctgcat tttggatgtt actaaatatt gtctgtttta actataaggc
     1321 aattttttat aacacccagg aaggcagcgc ggtggggctg tttttgcgct cacgcggcca
     1381 gcggaggccg cattcgccgg ctcgaccaga tggcggcgta gtcggcagca ctcaccgcgc
     1441 gcacgtcgtc gagcagttcg ccgagctcga cggcggtgag cgcgcggctg aaccagtggc
     1501 cctggatggc atcgcatccg gtgcggcgca tgaactccag ctgctcggcg ttttccacgc
     1561 cctcggcaac agtcttgatc gacagcgagc gcgccatggc gatgatggcg cgcgtgatcg
     1621 aggcggcgtc ggcgtcgctg cacacgtcgg cggtaaagga ctggtcgatc ttgatcacgt
     1681 cgatggggaa acgcttgaga tacgacaggc tggaatagcc ggtgccgaag tcgtccatcg
     1741 ccagcaccac gcccatgtct tccagttcgc gcagcgtttc gatcaccgca tcggagttgt
     1801 tgatcagcgt gctctcggtc agctccagtt ccagccgcga cggcggcaag ccggtgtcgc
     1861 ggcagacgct gcgcaccagc gccggcacat cctgctggtg aaactgccgc gccgagatgt
     1921 tcacggccac ggacagcgcc gcgtggccgg cgtcgcgcca ggcctgcagt tgcgcacagg
     1981 cgctgcgcag cgcccactcg ccgatcggca ggatcaggcc ggtatcctcg gccaggcgga
     2041 tgaagcggcc cggcggcacc gcgccgaact tcggatgctg ccagcggatc agcgcttcca
     2101 cgccgaagat gccgccggtc ttgatgtcca cctgcggctg gtaatgcagc gacagttcgt
     2161 cgcgcgccag cgcctggcgc agcgcggctt ccatttccac gcgctcgtcg gtctgcgcgc
     2221 tcatttcctg cgtgtagaac ttgtagccgc tgcggccgag ttccttggcg cggtacatgg
     2281 ccacgtcggc gtgcttgagc aatgtttcga aattctcgcc gtcctgcgga tacacgctga
     2341 tgccgatgct ggcggagacg tgcagctcgc ggccatcggc cgacagcggc tgcgacagcg
     2401 cggccagcag cttgccggca atggcgctga tggcatcgcg cgcggcaatg tcccacagca
     2461 tgatgacgaa ttcgtcgccg cccaggcgcg ccacggtgtc gccctcgcgc accgcctggg
     2521 tcagccggcc cgccaccgag cgcagcagcg catcgcccag gttgtggcca aagctgtcat
     2581 tgatgaactt gaagccgtcg agatccatga acatcaccgc gagcgagtgc ccggtgcgcc
     2641 gcgcctgcgc cagcgcctgc gccacgcggt cattcaacag attccggttc ggcagcccgg
     2701 tgagatcgtc gtggttggcc aggtagctca tctcggcttc atggctcttg cgctcggaga
     2761 tgtcgctggc cacgccgtcg aggcgcagcg gctggccctg cgcgtcgcgc accagccgcg
     2821 cgcgatcctc cagccagcgc acttcaccac cggggcggac gatgcggtac tgcagcgttg
     2881 ccgcgccctg ctccaccagc tgtgccagcc agtggcgcac gctggcgcgg tcatcgacgt
     2941 ggatcacctt gagccacagc agcgggtcgg actggaattc ggatacgggc cggccgtgca
     3001 cgcgctccac cgccgcgctc acgtactgca tctcgtggct cagcgcgttg accgaccaga
     3061 caatgttgtc gatcgactcg aggatgcccg acagcttttc ctccgccgcg tgcagctggt
     3121 ccacgcgctg ggtcaggctg gcattggcgc gggtgagctc gtcgcgcgca tggcgcaggc
     3181 cctcgcgcgc gccgtgg
//
LOCUS       DASZZC010000697         4041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1210736,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000697 DASZZC010000000
VERSION     DASZZC010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210736"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..2703
                     /locus_tag="VHE37_14760"
     CDS             166..2703
                     /locus_tag="VHE37_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVT36849.1"
                     /translation="MTMLLCAPVYADQYRSEVRELDTPPPEQPKQDETKLLQQALDPY
                     QKALVLRDLAAAAAGRKDYAEAAKLLEQALAQSGLSGPAADEMKRNLAQLYLATGNYQ
                     KVMPQLEAQVKAGNAPAETYIALGAAYADQKKFKDAIPLLQKGIAASKAPDKSWKRAL
                     ISAMYQAGQTRDLLPQLEQFLKEDPTHAEDWERVIALNLKAGNKERAYAYLDLAARLG
                     FLDSGEQKLQLVNLTAQLGAPFQAGSLLQTWMDKGELPKNNDNWRYLATLWVNAREQK
                     LALPALEQAVTHTPSKDMYLQIGQIHMDREEYGPAAAALEQAIALGAKNGATYLTLGM
                     ARYQQADVDGALKAFTEAEGYKDQTKLATQWAQYLESGKAREQALAAVSLRRTRNDSA
                     VALASGIGGSHIVLGADEGGASPSVTRAPLNGGDPLTPVGADRPGNVDNSIPAWTGGM
                     LPSEKPAAYNGTKVVDPFPSDRPLYVVTAANMAQYKDKLSRGHLALLHKYPDYQMPVY
                     VTRRTASYPQPIYDATQVNIGKAKLLGSDALSGAHLGFPFPHPQSGVEIMWNHRTRYR
                     GDQVSATYSQAVVAPDGSIREPNKTTFKVWFRYGNVKDPIDLAQQNILLYGITYGAPA
                     GHSPDYVALFHETANSIKQPRNIWVLIVKLQRMLRIPPVGYDQPFPASEGLMFIDMVD
                     MYNGAFDRYVWKLQGKREMLIPYNDYRLSDGHLKNAQLLTPKHFNQKDARYELHRVWV
                     IEATERGGMHHVFGKRTFYVDEDSWNVVLVENEDHDGNLWRFQEGHLVQLYDVQAMTA
                     LPDLTYDLKDGRYFANRLFAEDGLFRYDEKMTANEFLPEAVKNRYGH"
     gene            2868..>4041
                     /locus_tag="VHE37_14765"
     CDS             2868..>4041
                     /locus_tag="VHE37_14765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT36850.1"
                     /translation="MPSANVIHLQQPTADPLMAAAGQKKKLRALLVEDSMADARLLRE
                     LVRDADPTLQPELTHVQTLAHATAAITGGNYDCVLLDLNLPDGSGVETVEAVRGASRE
                     IAIIVLSGLNEEGLALQALRRGAQEYALKGQYDGDALLRIMHHAVERNRVFADVDRQR
                     EREYFLATHDPLTGLPNRQMLLDHARLAITQAERHKGGLALCFVDLDNFKPVNDTHGH
                     AIGDALLCEVGSILSQSVRDGDTVARTGGDEFIVLLTSVSSAEEAQAVSQRMADRISS
                     IRELKGCAINIGASIGLAVYPEMGKSFEELMANADVAMYEVKRGRGGGVGRARGAVPA
                     VRTERAAQDAVLYFQPWWDASRRSYGGVEVLPRAAGRQGEPFKFADSSAAPLTAGRL"
BASE COUNT          773 a         1359 c         1346 g          563 t
ORIGIN      
        1 gacccgcgaa gtgatccaga aggtcgagat ggacccggcc gactgcgcgt acaactggca
       61 atgaaaaatt caaagaactt ttcattgcca tccccgcgca ggcgcggatc aagttttgat
      121 ttcccgctct ggtggaaaga aaactggatg ccggcctgcg ccgccatgac gatgctgttg
      181 tgcgcgccgg tctacgccga ccaataccgc agcgaggtgc gcgagctcga cacgccgccg
      241 ccggagcagc ccaagcagga cgaaaccaag ctgctgcagc aggcgctcga cccgtaccag
      301 aaggcgctgg tgctgcgcga cctcgccgcc gccgcggccg gccgcaagga ctacgcggag
      361 gcggcgaagc tgctcgagca ggcgctggcg cagagcgggt tgtccggccc cgcggccgat
      421 gagatgaagc gcaacctggc gcagctgtac ctggccaccg gcaactacca gaaggtgatg
      481 ccgcaactcg aagcgcaggt gaaggccggc aacgcgccgg cggaaaccta catcgctctg
      541 ggcgccgcct atgccgacca gaagaagttc aaggacgcca tcccgctgct gcagaagggc
      601 atcgcggcga gcaaggcgcc ggacaagagc tggaagcgcg cgctgatctc cgccatgtac
      661 caggccggcc agaccaggga cctgctgccg caactcgaac agttcctcaa ggaagacccc
      721 acgcacgcgg aggactggga gcgggtcatc gcgctcaacc tcaaggccgg caacaaggag
      781 cgcgcctacg cctatctcga tctcgccgcg cggctgggct ttctcgacag tggcgagcag
      841 aagctgcagc tggtgaatct caccgcgcag ctcggcgcgc cgttccaggc cggctcgctg
      901 ctgcagacct ggatggacaa gggcgagttg ccgaagaaca acgacaactg gcgctacctg
      961 gccacgctgt gggtcaacgc gcgcgagcag aaactggcgc tgccggcgct cgaacaggcg
     1021 gtgacccata cgccgagcaa ggacatgtac ctgcagatcg gccagatcca catggaccgc
     1081 gaggagtacg gtccggcggc ggcggcgctg gagcaggcta tcgcgctggg cgcgaagaac
     1141 ggcgccacct atctcacgct gggcatggcg cgctaccagc aggcggacgt cgacggcgcg
     1201 ctcaaggcct tcaccgaggc cgagggctac aaggaccaga cgaaactggc gacgcaatgg
     1261 gcgcagtacc tcgagagcgg caaggcgcgc gagcaggcgc tggccgcggt gtcgctgcgg
     1321 cgcacgcgca acgacagcgc cgttgcgctg gccagcggaa tcggcggcag ccatatcgtg
     1381 cttggtgccg acgagggcgg ggcgtcgcca tcggtcacgc gtgcgccgct caatggcggc
     1441 gatccgctga cgccggtcgg cgcggaccgg ccgggcaatg tcgacaacag catcccggcc
     1501 tggaccggcg gcatgctgcc cagcgagaag ccggccgcgt acaacggcac gaaagtggtc
     1561 gacccgtttc ccagcgacag gccgctgtac gtggtcacgg ccgcgaacat ggcgcaatac
     1621 aaggacaagc tctcgcgcgg gcatctcgcc ttgctgcaca aatacccgga ctaccagatg
     1681 ccggtgtacg tcacccgccg caccgcttca tatccgcaac cgatctacga cgccacccag
     1741 gtcaacatcg gcaaggccaa gctgctgggc tccgacgcgc tgtccggcgc acacctcgga
     1801 tttccgtttc cgcatccgca gtcgggcgtg gagatcatgt ggaaccaccg cacgcgctac
     1861 cgcggcgacc aggtgtccgc cacctacagc caggcggtgg tggcgcccga cggcagcatc
     1921 cgcgagccga acaagaccac gttcaaggtg tggttccgct acggcaacgt caaggatccg
     1981 atcgacctgg cgcagcagaa catcctgctc tacggcatca cctacggcgc gccggcgggg
     2041 cactcacccg attacgtggc gctgttccac gagacggcca actcgatcaa gcaaccgcgc
     2101 aacatctggg tgctgatcgt gaagctgcag cgcatgctgc gcattccgcc ggtgggctac
     2161 gaccagccgt tcccggcctc ggaagggctg atgttcatcg acatggtcga catgtacaac
     2221 ggcgccttcg accgctacgt gtggaagctg caaggcaagc gcgagatgct gatcccgtac
     2281 aacgactatc gcctttccga tgggcacctg aagaacgcgc aactgctgac gccgaagcac
     2341 ttcaaccaga aggatgcccg ttacgagctg caccgcgtgt gggtgatcga ggccaccgag
     2401 cgcggcggca tgcaccacgt gttcggcaag cgcacgttct atgtcgacga ggacagctgg
     2461 aacgtggtgc tggtggagaa cgaggaccac gacggcaacc tgtggcgctt ccaggaaggc
     2521 cacctggtgc agctgtacga cgtgcaggcc atgaccgcgc tgccggacct cacctacgac
     2581 ctcaaggacg ggcgctactt cgccaaccgc ctgttcgccg aagacggcct gttccgctac
     2641 gacgagaaga tgaccgccaa cgagttcctg cccgaggcgg tgaaaaaccg ttacggccac
     2701 tagcgcatgg accgtacgcc cgcgcctggc gccaaccggc tggcggcgcc ggcacggccg
     2761 cctgtctttt ccacaggggg attagactat ttctctatca agtcccggcg ggcggagcag
     2821 acaccccgat tagactcact gccgccgcac gacatggaga gtcgtcgatg cccagcgcca
     2881 acgtaataca tctgcagcag ccgacagcgg acccgctgat ggccgcggct gggcagaaga
     2941 aaaagctgcg cgcactgctg gtcgaggatt cgatggccga cgcgcgcctg ttgcgcgaac
     3001 tggtgcgcga tgccgacccc acgctgcagc cggaactgac ccacgtgcag acgctggccc
     3061 atgccactgc cgccatcacc ggcggcaact acgactgcgt gctgctcgac ctcaacctgc
     3121 ccgacggcag cggggtggaa acggtggaag cggtgcgcgg cgccagccgc gagatcgcca
     3181 tcatcgtgct gtccggcctg aacgaggaag ggctggcgct gcaggcgctg cgccgcggcg
     3241 cgcaggagta cgcgctcaag ggccagtacg acggcgacgc gctgctgcgc atcatgcacc
     3301 acgcggtgga gcgcaaccgc gtgttcgccg atgtcgaccg ccagcgcgag cgcgaatatt
     3361 tcctggccac gcacgatccg ctcaccggcc tgcccaaccg gcagatgctg ctggaccacg
     3421 cgcgcctggc cattacccag gccgagcgtc acaagggcgg cctggcgctg tgcttcgtcg
     3481 acctggacaa cttcaagccg gtgaacgaca cccacggcca cgccatcggc gatgcgctgc
     3541 tgtgcgaagt gggcagcatc ctgtcgcagt cggtgcgcga cggcgacacc gtggcgcgta
     3601 ccggcggcga cgaattcatc gtgctgctca cctcggtgag ctcggccgag gaggcccagg
     3661 ccgtcagcca gcgcatggcc gaccgcatca gttcgatccg tgaactcaag ggctgcgcca
     3721 tcaacatcgg cgccagcatc ggcctggcgg tctacccgga gatgggcaag agcttcgagg
     3781 agttgatggc caacgccgac gtggccatgt acgaggtcaa gcgcgggcgc ggcggcggcg
     3841 tcggccgcgc gcgcggcgcg gtgccggcgg tgcgcaccga gcgcgccgcg caggatgcgg
     3901 tgctgtattt ccagccctgg tgggatgcgt cgcggcgcag ctatggcggt gtcgaagtgc
     3961 tgccgcgtgc ggccggccgc cagggcgagc cgttcaagtt tgccgacagc agcgccgcgc
     4021 cgctcaccgc cggccggctg g
//
LOCUS       DASZZC010000698         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_824001,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000698 DASZZC010000000
VERSION     DASZZC010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824001"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VHE37_14770"
     CDS             complement(<1..458)
                     /locus_tag="VHE37_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVT36851.1"
                     /translation="MGASAAVFAAPIADYLDYLTHQRKYSPLTRGGRERDLQRFAQWC
                     AASRRMRLDQIDTHAVRGYAAALHRDGLEPVTVQRHLSSLRSFLRFHVQRGALPANPA
                     DGVRAPKVRRKLPGVIAAEPLTAALDAPVDGDLLLRDRAMVELFYSTGLRL"
     gene            complement(462..1172)
                     /locus_tag="VHE37_14775"
     CDS             complement(462..1172)
                     /locus_tag="VHE37_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HVT36852.1"
                     /translation="MAEQLKTAKIEKPEAAPYSEAEVADFLKARPDFFSRYPDLLESI
                     ELAHRAGSAVSLIEKQVDNQRARNQKLEEKLTRLLDAARDNEKRADHVHKLARTLLRA
                     PSLAGAIAGLRQCMREDFGIDEIFVGINAKHYRRHDIEGLTPLEAEGRIALGFVDFFR
                     TRLTECGPIAEQKSRLLFGKCEPLPQSAAIVPLERDKNLGMLAFGSRDPERFQPRQGK
                     MFLEMTAELVAAAMRARL"
     gene            complement(1157..1993)
                     /gene="dapF"
                     /locus_tag="VHE37_14780"
     CDS             complement(1157..1993)
                     /gene="dapF"
                     /locus_tag="VHE37_14780"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HVT36853.1"
                     /translation="MKLRFTKMHGAGNDFVVIDATREPFKPTPALLARLADRRFGVGC
                     DQVLVVDPPSSGVDFNYRIYNSDGSESGQCLNGARCFARFVREQGLSDKNDIRIRTRA
                     STMELHLLADGRVRINAGVPLLDPERIPFRAARAPRYTLTLADGSQVEIGAVGMGNPH
                     AVMLADDVERAPVEARGKLLQRHAAFPESVNAGFLQVVDPSHAKLRVYERGAGETLAC
                     GSGACAAVVAGRLWGVLGSRVELQMPGGKLVVEWEGEGRPVWLTGPAVSVYTGEFEWQ
                     NS"
     gene            2028..2318
                     /locus_tag="VHE37_14785"
     CDS             2028..2318
                     /locus_tag="VHE37_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36854.1"
                     /translation="MMRMSRVRTAAMLLALLLGQWLNLAHGFIHPVDAGPDAHCQMCL
                     HAQGMDSGAIAPVTHLPPLAVSHEIPALPLRRAVISIAADGHRIRGPPLLVG"
     gene            2480..>3269
                     /locus_tag="VHE37_14790"
     CDS             2480..>3269
                     /locus_tag="VHE37_14790"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT36855.1"
                     /translation="MSGFTTFAAAADDKDQPVDLGNITVHASPFGRAADDLTQPVDVL
                     AGTQLERKRKTTIGETLEGELGVATSDFGPGVGRPIIRGQAGPRVQVLENGIGSMDLS
                     SASDDHAVTIDPADAQQVEIIKGPATLIYGSGASGGVVNVVNNRLAEKYDDGLHARVD
                     ASYADNGNDRNSSANVDYGLAGTMIHLDGAERRALGDFKIPGNTQPDGGGTQGYIPNS
                     AVRTQSVGVGATNLGDWGSVGMAASLYNNRYGVPGEVGTTKYLDM"
BASE COUNT          511 a         1142 c         1096 g          520 t
ORIGIN      
        1 aggcgcaggc cggtggaata gaacagctcg accatggcgc ggtcgcgcag cagcaggtcg
       61 ccgtccaccg gcgcatcgag cgcggcggtg agcggctcgg cggcgatgac gccaggcagc
      121 ttgcgccgta ccttgggcgc gcgcacgccg tccgccggat tcgccggcag cgcgccgcgc
      181 tgcacgtgga agcgcaggaa cgagcgcagc gacgaaagat gccgctgcac cgtcaccggc
      241 tcgagcccat cgcgatgcag cgccgccgcg tagccgcgca ccgcgtgggt gtcgatctgg
      301 tcgagtcgca tgcggcgcga ggccgcgcac cattgcgcga agcgctgcag gtcgcgctcg
      361 cgtccgccac gcgtcagcgg cgagtacttg cgctggtggg tgaggtagtc gagatagtcc
      421 gcgatcggcg ctgcgaacac cgcggccgat gcgcccacgg actagagccg cgcgcgcatg
      481 gcggcggcga ccagttccgc ggtcatttcc aggaacatct tgccctggcg cggctggaag
      541 cgttccgggt cgcggctgcc gaaggccagc atgcccaggt tcttgtcgcg ttccagcggc
      601 acgatggcag cggactgcgg cagcggctcg cacttgccga acagcaggcg ggatttctgc
      661 tcggcaatcg gcccgcattc ggtcaggcgc gtacggaaga aatcgacgaa accgagcgcg
      721 atgcgcccct cggcttccag cggcgtcagg ccttcgatgt cgtggcggcg gtaatgcttg
      781 gcattgatgc cgacaaagat ttcgtcgatg ccgaagtcct cgcgcatgca ctggcgcagg
      841 ccggcgatgg cgcccgccag cgacggcgcg cgcagcagcg tgcgcgccag cttgtgcacg
      901 tggtcggcgc gcttctcgtt gtcgcgcgcg gcatcgagca ggcgcgtgag cttttcctcc
      961 agcttctggt tcctggcgcg ctggttgtcg acctgcttct cgatcagcga caccgccgag
     1021 ccggcgcggt gcgccagctc gatcgactcc agcaggtcgg gatagcgcga gaagaaatcc
     1081 gggcgcgctt tcaggaaatc cgccacctcg gcttcgctgt acggcgctgc ttcaggtttt
     1141 tcgatcttcg cggtcttcag ctgttctgcc actcgaattc ccccgtgtac acgctcaccg
     1201 ccggaccggt cagccagacc gggcggccct cgccttccca ttccaccacc agcttgccgc
     1261 cgggcatctg cagctctacg cgcgaaccga gcacgcccca cagccggccg gccaccaccg
     1321 ccgcgcaggc gccgctgccg caggccagtg tctcgccggc accgcgctca tacacgcgca
     1381 gcttcgcatg cgatggatcg accacctgca ggaagccggc attgaccgat tccgggaacg
     1441 ccgcgtgccg ctgcaggagt ttgccgcgcg cttccaccgg cgcgcgctca acgtcatccg
     1501 ccagcatcac tgcgtgcggg ttgcccatgc ccacggcgcc gatctcgacc tggctgccgt
     1561 ccgccagcgt cagcgtgtag cgcggcgcac gcgcggccct gaacgggatg cgctcgggat
     1621 cgagcagcgg cacacccgcg ttgatgcgca cgcggccgtc ggccagcagg tgcagctcca
     1681 tggtgctggc gcgggtgcgg atgcggatat cgttcttgtc cgacaagccc tgctcgcgca
     1741 cgaagcgcgc gaagcagcgc gcgccgttca ggcactggcc ggactcggag ccgtcgctgt
     1801 tgtagatacg gtaattgaag tcgacgccgg acgacggcgg gtccaccacc agcacctggt
     1861 cgcagccgac gccgaagcgg cggtccgcca gccgggccag cagcgctggc gtgggcttga
     1921 acggctcgcg cgtggcatcg atgacgacaa agtcgttgcc ggcaccgtgc atcttggtaa
     1981 agcgcagctt catgccgcgc attttacggc tgttccgcta gaatcgcgtg atgcgcatga
     2041 gccgtgtccg taccgctgcg atgctgctgg ccctgctgct gggccagtgg ctgaacctcg
     2101 cgcacggctt catccacccg gtcgacgcgg ggcccgacgc gcactgccag atgtgcctgc
     2161 atgcgcaggg catggactcg ggcgccatcg ctcctgtcac gcatctgccg ccgcttgccg
     2221 tctcgcatga aattccggcg ctgccgctgc gccgcgccgt catcagcatt gccgcggacg
     2281 gacatcgcat ccgcggccct cctctcctcg tcggctaaag cccggacgcg tgcggccgca
     2341 ctacctgcgg ccgccgctcc tttttctttt tggactttcc gatggggatt tcatgtctac
     2401 tttaaattcc ggcagccgtg gctgccgtgc agtgcggcag gctcgtgccc gccatgggtt
     2461 attcatgctg tgcgccgcca tgagcggatt caccactttt gccgctgcgg ccgacgacaa
     2521 ggaccagccg gtcgacctgg gcaacatcac cgtgcatgcc agcccattcg gccgcgcggc
     2581 tgacgatctg acgcagccgg tcgacgtgct ggccggcacg cagctcgagc gcaagcgcaa
     2641 gaccacgatc ggcgaaacgc tggaaggcga actcggcgtc gccacgtccg atttcggccc
     2701 cggcgtcggc cggcccatca tccgcggcca ggccgggccg cgcgtgcagg tgctggaaaa
     2761 cggtatcggc tcgatggacc tgtcgtcggc cagcgacgat cacgcggtga ccatcgaccc
     2821 ggccgacgcg cagcaggtgg agatcatcaa gggcccggcc acgctcatct acggcagcgg
     2881 cgcgtccggc ggcgtggtca acgtggtcaa caaccggctg gcggaaaagt acgacgatgg
     2941 cctgcacgcg cgcgtcgatg cgtcgtatgc cgacaacggc aatgaccgca acagctcggc
     3001 caatgtcgac tacggcctcg ccggcaccat gatccacctc gatggcgcgg agcggcgcgc
     3061 gctgggcgac ttcaagatcc ctggcaacac gcagcccgat ggcggcggca cgcagggcta
     3121 catccccaac agcgcggtgc gcacgcaatc ggtcggcgtt ggcgccacca acctgggcga
     3181 ctggggcagc gtgggcatgg cggccagcct gtacaacaac cgctatggcg tgccgggcga
     3241 ggtgggcacc accaagtacc tcgacatgt
//
LOCUS       DASZZC010000699         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_627434,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000699 DASZZC010000000
VERSION     DASZZC010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627434"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHE37_14795"
     CDS             complement(<1..295)
                     /locus_tag="VHE37_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HVT36856.1"
                     /translation="MGHKVNPNGFRLGITSQWSSNWYAERGIYTDNLNKDIKVRKFLQ
                     EKLAQANVSRVQIDRPAKSARVTIHTARPGIVIGKKGEDIEKLKQEVAQKMGLK"
     gene            complement(306..638)
                     /gene="rplV"
                     /locus_tag="VHE37_14800"
     CDS             complement(306..638)
                     /gene="rplV"
                     /locus_tag="VHE37_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HVT36857.1"
                     /translation="MKTQAVLKFTRLAPNKARLLADLVRGKKVDEALNILKFSNQKSA
                     KVIRKVLESAIANAENNNGADVDELKVREIFVDEGPVMKRIMPRAKGRADRIVKPTSH
                     ITVRVSDE"
     gene            complement(650..961)
                     /gene="rpsS"
                     /locus_tag="VHE37_14805"
     CDS             complement(650..961)
                     /gene="rpsS"
                     /locus_tag="VHE37_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HVT36858.1"
                     /translation="MPRSLKKGPFVDHHLAKKVTDMQANRVKKPIKTWSRRSMVTPDM
                     VGLTIQVHNGRQHMPVFITENMISHKLGEFAPTRIFKGHGERKTAEAAAPKPAAAAPA
                     A"
     gene            complement(974..1804)
                     /gene="rplB"
                     /locus_tag="VHE37_14810"
     CDS             complement(974..1804)
                     /gene="rplB"
                     /locus_tag="VHE37_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HVT36859.1"
                     /translation="MPLIQMKPTSPGQRHVVRVVHEGLHKGEPYAPLLAKKNQTGGRN
                     NNGHITTRHKGGGHKQHYRLIDFKRDKDGVPGKVERLEYDPNRSAHIALVLFADGERR
                     YIIAARGMAEGDTVQNGSDAAIKPGNALPLRNIPVGSTIHCVEMRPGKGAQLARSAGA
                     GVQLVSRDGDYATIRLRSGETRKVHSDCRAAVGEVCNADHNLRKFGKAGAKRWQGIRP
                     TVRGVVMNPVDHPHGGGEGKSGQGNPHPVTPWGQQTKGLKTRNNKRTQNMIVRSRHKR
                     "
     gene            complement(1817..2119)
                     /gene="rplW"
                     /locus_tag="VHE37_14815"
     CDS             complement(1817..2119)
                     /gene="rplW"
                     /locus_tag="VHE37_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HVT36860.1"
                     /translation="MNVDRLHQIIVAPVVSEKSTRVAEKRNQAVFKVKPDAQKAEIKQ
                     AVEALFNVKVTGVQTLNVKGKNKRFGQSAGRRSDWKKAYVTLAEGQQIDFAGGTAA"
     gene            complement(2116..2721)
                     /gene="rplD"
                     /locus_tag="VHE37_14820"
     CDS             complement(2116..2721)
                     /gene="rplD"
                     /locus_tag="VHE37_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HVT36861.1"
                     /translation="MDIQLTNSSNKLSVSDAVFGADYNEALIHQVVTAYMNGGRAGTK
                     AQKSKGMVRGGGKKPWKQKGTGQARAGSIRSPLWRGGGKTFAAVPRDFSQKVNKKMYR
                     GALRAIVSELARQGHLIVAESFAVDAPKTKGLQAQLAKIGSNDILIVTGEVNQNLFLS
                     SRNLPHVGVCDVEAINPVALLTHQKVLMTQDAVKKLEAWLS"
     gene            complement(2740..3375)
                     /gene="rplC"
                     /locus_tag="VHE37_14825"
     CDS             complement(2740..3375)
                     /gene="rplC"
                     /locus_tag="VHE37_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HVT36862.1"
                     /translation="MTIAIVGRKAGMTRVFTESGEGIPVTVIECTPNRVTQVKTTETD
                     GYRAVQVAVGEKKASRVNKALTGHYKKSGVAAGRGLWEIRLDESEGKDLAPGSEIKVD
                     AFNGVKAVDVTGTSIGKGFAGVIKRHHFGGGRATHGNSLSHRSPGSIGQRQSPGKVWK
                     NKKMAGHLGNVQVTTLNLDLVKIDTERHLLLVKGAVPGSANGDVIVRPTVK"
     gene            complement(3387..3704)
                     /gene="rpsJ"
                     /locus_tag="VHE37_14830"
     CDS             complement(3387..3704)
                     /gene="rpsJ"
                     /locus_tag="VHE37_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HVT36863.1"
                     /translation="MAATSQSIRIRLKAFDHRLIDKSAREIVETAKRTGAVVRGPIPL
                     PTRKERYTILVSPHADKDARDQYEIRTHKRLMQIVDPTEKTVDALSKLDLPAGVEVQI
                     KVN"
     gene            complement(3793..>3937)
                     /gene="tuf"
                     /locus_tag="VHE37_14835"
     CDS             complement(3793..>3937)
                     /gene="tuf"
                     /locus_tag="VHE37_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HVT36864.1"
                     /translation="QEMVMPGDNVKVVVELISPIAMEEQVRFAIREGGRTVGAGVVTK
                     ILA"
BASE COUNT          617 a         1248 c         1207 g          865 t
ORIGIN      
        1 acttcaggcc catcttctgc gccacttcct gcttcagctt ctcgatgtcc tcgcccttct
       61 tgccgatgac gatgccagga cgcgccgtgt ggatggtgac gcgcgccgac ttggccggac
      121 ggtcgatctg cacgcgcgag acgttggcct gcgccagctt ctcctgcagg aacttgcgaa
      181 ccttgatgtc cttgttcagg ttatcggtgt aaatgccgcg ctccgcgtac cagttggagc
      241 tccactggct ggtgatgccg aggcgaaaac cgttcgggtt gactttatgg cccatgatcg
      301 attccttact cgtccgacac gcgaacggtg atgtggctgg tcggcttgac gatgcgatcg
      361 gcgcggcctt tggcgcgcgg catgatgcgc ttcatcaccg gcccctcgtc gacgaagatc
      421 tcgcggacct tcagctcgtc aacatcggcg ccgttgttgt tctcagcgtt ggcaatggcg
      481 gactcgagca ccttgcggat caccttggcc gatttctggt tcgaaaactt gaggatgttg
      541 agagcctcgt caaccttctt gccccggacc agatcagcca gcaggcgcgc cttgttgggc
      601 gccagccggg tgaacttgag cactgcttgc gtcttcatga acgggctcct taggcggccg
      661 gcgcggctgc agccggctta ggcgcagcgg cttcggccgt cttgcgctcg ccgtggccct
      721 tgaagatgcg cgtgggcgca aactcgccga gcttgtggct gatcatgttc tcggtgatga
      781 acaccggcat gtgctggcgg ccattgtgca cctggatggt caggccgacc atgtcaggcg
      841 tcaccatcga acggcgcgac caggtcttga tcggcttctt gacgcggttg gcctgcatgt
      901 cggtcacctt tttggcgagg tgatgatcga cgaacggacc tttcttgagt gaacgtggca
      961 tggcgtcagt ctcttagcgc ttgtgacggc tgcgcacgat catgttctgc gtgcgcttgt
     1021 tgttgcgggt cttgagaccc ttggtctgct ggccccacgg ggtgaccggg tgcgggttac
     1081 cctggccgga tttgccttcg ccaccgccgt gcgggtggtc aaccgggttc atcaccacgc
     1141 cgcggaccgt cggacggatg ccctgccagc gcttggcgcc ggctttgccg aacttgcgca
     1201 ggttgtggtc ggcgttgcac acctcgccca ccgccgcgcg gcaatccgaa tgcaccttgc
     1261 gcgtctcgcc ggaacgcagg cggatggtgg cgtagtcgcc gtcacgcgaa accagctgca
     1321 cgccagcgcc ggctgagcgc gccagctgcg cacccttgcc cggacgcatc tcgacgcagt
     1381 gaatggtgga acccaccggg atgttgcgca gcggcagggc attgcccggc ttgatcgcag
     1441 catcggaacc gttctgcacc gtgtcgccct cggccatgcc gcgggcggcg atgatgtaac
     1501 ggcgctcgcc gtcggcgaac agcaccagcg cgatgtgcgc gctgcggttc ggatcgtact
     1561 cgagacgttc gaccttgcca ggaacgccgt ccttgtcgcg cttgaagtcg atcaggcgat
     1621 agtgctgctt gtgcccgccg cccttgtgac gcgtggtgat gtggccgttg ttgttgcggc
     1681 cacccgtctg gttcttcttg gccagcaacg gtgcatacgg ttcgcccttg tgcaggccct
     1741 catgaaccac gcgcacaaca tggcgctggc cgggcgaggt gggcttcatt tgaatgagtg
     1801 gcatgacctt gcttccttac gccgcggtac cgccggcgaa atcgatctgc tggccttcgg
     1861 ccagcgtgac gtaggctttt ttccagtccg agcgacgccc ggccgactgg ccaaaccgct
     1921 tgttcttgcc cttgacgttt agcgtctgca cgccggtgac cttgacattg aacaacgcct
     1981 cgacggcctg cttgatctcg gccttctgcg cgtccggctt gaccttgaac accgcttggt
     2041 tgcgcttctc ggccacgcgc gtgctctttt cagagacgac gggcgcgacg atgatctgat
     2101 ggagacgatc gacgttcatg acagccaggc ctcaagtttt ttcaccgcgt cctgtgtcat
     2161 cagcaccttc tggtgcgtga gcagggccac ggggttgatc gcctcgacat cgcacacgcc
     2221 gacatgcggc agattgcgcg acgacaggaa caggttctgg ttgacctcgc cggtgacgat
     2281 caggatgtcg ttggagccga tcttggccag ctgcgcttgc aagcccttgg tcttcggcgc
     2341 atcgacggca aacgactcgg cgacgatcag gtgaccctgg cgggccagct ctgacacgat
     2401 cgcgcgcagc gcgccgcgat acatcttctt gttgaccttc tgcgagaaat cacgagggac
     2461 cgcggcaaaa gtcttgccgc cgccgcgcca cagtggcgag cggatcgaac cggcgcgcgc
     2521 ctgaccggtg cccttctgct tccacggttt cttgccgccg ccacgcacca tgcccttgct
     2581 cttctgggcc ttggtgccgg cgcggccgcc attcatgtag gcggtgacca cctggtggat
     2641 cagggcttcg ttgtaatcgg ctccgaacac ggcgtccgat acggaaagct tgttgctgct
     2701 attcgtcagt tgtatatcca ttgccgtttt tccccagtct cacttgaccg tggggcgcac
     2761 gatgacgtcg ccgttcgcgc tgcccggcac cgcgcccttc accaaaagga gatggcgctc
     2821 ggtgtcaatc ttcaccaaat ccagattcag ggtggtcacc tgcacattgc ccagatggcc
     2881 agccatcttc ttgttcttcc acaccttgcc cggactctga cgctgaccga tagaaccagg
     2941 agaccggtgc gacagcgagt taccgtgcgt tgcgcgacca ccaccgaaat ggtgccgctt
     3001 gattacaccg gcaaagccct tgccgatgct ggtgccggtg acgtccacgg ccttcacgcc
     3061 gttaaacgca tcgaccttga tttcgctgcc gggagcaagg tctttaccct cgctctcgtc
     3121 gagacggatt tcccacaggc cgcgacccgc ggctacgccg gacttcttgt aatggccggt
     3181 cagcgccttg ttgacgcggc tggccttctt ctctcccacc gccacctgca cggcacgata
     3241 gccgtcggtt tccgtggtct tgacctgcgt cacgcggttg ggcgtgcact cgatcaccgt
     3301 gaccggtatg ccttcgccgc tctcggtgaa gacccgggtc atgccggcct tgcggcccac
     3361 gattgcaatc gtcatggcgc cgttcctcaa ttgaccttga tctggacttc aacgcccgca
     3421 ggcaggtcca gcttggaaag ggcatcgacc gtcttttcgg tcggatcgac gatctgcatc
     3481 aggcgcttgt gcgtgcggat ctcatactgg tcgcgcgcgt ccttgtcggc atgcggcgat
     3541 accaggatgg tgtagcgctc cttgcgcgtc ggcagcggga tcgggccgcg aaccacggca
     3601 ccggtacgct tggcggtctc gacgatttca cgcgccgatt tgtcgatcag gcggtggtcg
     3661 aaagccttga gccggatacg aatagattgg ctagtcgcgg ccatgctgtt ttccaggtct
     3721 taaagaacgg ttgaagcaaa ggggcgcgga ttatacgcac gccccccgga tgagtaaaga
     3781 acttttcgag gcttaggcca gaatcttggt aacgacgcct gcgccgacgg tcctgccgcc
     3841 ctcgcggatg gcaaaacgca cctgctcttc catggcgatc gggctgatca gttcgaccac
     3901 caccttgacg ttgtcgcccg gcatcaccat ttcctgg
//
LOCUS       DASZZC010000700         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1023703,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000700 DASZZC010000000
VERSION     DASZZC010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023703"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VHE37_14840"
     CDS             <1..579
                     /locus_tag="VHE37_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT36865.1"
                     /translation="SADNENLVKVAEQKFGALHVMFNNAGIMDSRDDDAMSTEEAVWD
                     LTMNVNAKSIFLACKFGIPALQRAGGGSIINTASIVALVGSAAPQVAYTASKGAILSL
                     TRELAVIHARQNIRINALCPGPLRTELLMKFLNTDAKKQRRLVHIPMGRFGESKEIAK
                     AALFLASDEASYTTGSTFVVDGGLTCAYITPE"
     gene            610..>2218
                     /locus_tag="VHE37_14845"
     CDS             610..>2218
                     /locus_tag="VHE37_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT36866.1"
                     /translation="MDSKLSLPRAALVAAFSFLAACSTPGSAPPAAAAAPAPAPAAAK
                     PTWDQHVAQFLEDYFSWNPNFAVSQGRHEFDGKLPDWTPAAFGREAQWLKDTQTATLA
                     FDPALLTPAQRFEREYLLARIDNELFWLTEAELPYTHPDYYLYGGLDPNVYISRPYGT
                     PEARMLAFIKYAHAVPAALKQVRSNLRLPLPKTFLDLAIKGFEGYASFFRDEVPKAFA
                     AAGDPATQSLLHKEIAAAAAAMQSTADWLRREQPKGTDDFALGPEKFADMLRMTERVN
                     TPLAELEALGKADLNRNLVALRTACAKFAPMRSIVQCIGKMAKNKPKGGAVDGARAQL
                     ADLRQFIVDHQLVSIPGDEAALVDEAPAYNRQNFAYIDIPGPYDKGMPSTYYIAPPDP
                     TWSKADQQAYIPGQADLLFTSVHEVWPGHFLQFLHANRSPSLFGQVFVGYAYAEGWAH
                     YGEEMMWEAGLGAGSPETHIGQLSNALLRNVRFLCSIGLHTGHMSVQTCEKMFREQAY
                     QDPGNARQQAARGTYDPAYLNYTLGKLM"
BASE COUNT          409 a          783 c          687 g          339 t
ORIGIN      
        1 tcagcggaca acgaaaacct ggtcaaggtg gccgagcaga agttcggcgc actgcacgtc
       61 atgttcaaca acgccggcat catggacagc cgcgacgacg acgccatgag caccgaggaa
      121 gcggtgtggg acctgaccat gaacgtcaac gccaagagca tcttcctggc gtgcaagttc
      181 ggcatacctg cgctgcagag agcgggcggc ggctccatca tcaacacggc gtcgatcgtg
      241 gcactggtcg gttcggccgc gccgcaggtg gcctacaccg cgagcaaggg cgccatcctg
      301 tcgctgacgc gcgaactggc ggtgatccat gcgcggcaga acatccgcat caacgcgctg
      361 tgccccggac cgctgcgcac cgagctgctg atgaagttcc tcaacaccga cgccaagaag
      421 cagcggcgcc tggtgcatat cccgatgggc cgcttcggcg agtcgaagga gatcgccaaa
      481 gccgcgctgt tcctggcctc ggatgaagcc agctacacca ccggcagcac cttcgttgtc
      541 gacggcggcc tgacttgcgc ttacatcacg cctgagtaag ctcgcggctt cactgccacg
      601 aagaaggcca tggattccaa gctctcgctg ccgcgtgcag cgctggtcgc cgcgttttct
      661 ttcctcgctg cctgcagcac gccgggcagc gcgccgcccg ctgccgcagc cgcccctgca
      721 ccggcgcccg cggcggcgaa gccgacctgg gatcagcatg tggcgcaatt cctcgaagat
      781 tatttttcgt ggaatccgaa cttcgcggtg tcgcagggcc gccatgagtt cgacggcaag
      841 ctgcccgact ggacccccgc ggcgttcggg cgcgaggcgc agtggctgaa ggacacacag
      901 accgccacgc tggcgttcga ccctgccctg ctgacaccag cacagcgctt cgagcgcgag
      961 tacctgctgg cgcgcatcga caacgaactg ttctggctga ccgaggccga gctgccgtac
     1021 acccacccgg actactacct ctacggcggg cttgatccca acgtgtacat ctcaaggccg
     1081 tacggcacgc ccgaagcgcg catgctggcc ttcatcaagt acgcgcacgc ggtaccggcc
     1141 gcgctcaagc aggtgcgctc caacctgcgc ctgccgctgc caaaaacttt cctcgacctg
     1201 gcgatcaagg gcttcgaggg ctatgccagc ttcttccgcg atgaagtgcc caaggccttt
     1261 gccgccgccg gcgaccccgc cacgcagtcg ctgctgcaca aggagatcgc cgcggccgcg
     1321 gctgcgatgc agagcaccgc cgactggctg cgccgcgagc agcccaaggg caccgacgac
     1381 ttcgccctcg gcccggaaaa attcgccgac atgctgcgca tgaccgagcg cgtgaacacg
     1441 ccgctcgccg aactcgaagc gctgggcaag gctgacctca accgcaacct cgtggccctg
     1501 cgcaccgcgt gcgcgaaatt cgcgcccatg cgcagcatcg ttcaatgcat tgggaagatg
     1561 gcgaagaaca agcccaaggg cggcgcggtg gacggcgcgc gcgcacagct ggccgacctg
     1621 cgccagttca tcgtcgacca ccagctggta tcgattccgg gcgacgaggc ggcgctggtc
     1681 gacgaggcgc cggcgtacaa ccggcagaac ttcgcctaca tcgacatccc cggcccctac
     1741 gacaagggca tgccctcgac ctactacatc gccccgcccg accccacctg gtccaaggcc
     1801 gaccagcagg cctacatccc gggccaggcc gacctgctgt tcacctcggt gcacgaggtg
     1861 tggccggggc acttcctgca gttcctgcac gccaaccgct cgccgtcgct gttcggccag
     1921 gtgttcgtcg gctacgccta cgccgagggc tgggcgcatt acggcgagga gatgatgtgg
     1981 gaggctggcc tgggcgccgg ctcgcccgaa acgcatatcg gccagctgtc gaatgcgctg
     2041 ctgcgcaacg tgcgctttct ctgctcgatc ggattgcaca ccggccacat gagcgtgcag
     2101 acctgcgaga agatgttccg cgagcaggcc taccaggacc cgggcaacgc gcgccagcag
     2161 gccgcgcgcg gcacctatga cccggcctat ctcaactaca cgctgggcaa gctgatga
//
LOCUS       DASZZC010000701         6007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_879040,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000701 DASZZC010000000
VERSION     DASZZC010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879040"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..732)
                     /gene="scpB"
                     /locus_tag="VHE37_14850"
     CDS             complement(19..732)
                     /gene="scpB"
                     /locus_tag="VHE37_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HVT36867.1"
                     /translation="MSDNEEPVAAAPVEAAAPVETTGDLPKILEALLLASELPLPLDH
                     LQRLLGSELGVGRDELRAGLEALARSLEGRAAELTEVHSGYRIQVRKEYAQWVSLLFQ
                     EKPPKLSRALMETLALVCYRQPITRGEIEEVRGVALSPNIVRVLVERGWIREVGQKEV
                     PGRPSLFGTTPQFLDDFNLKSLDDLPSLPEIKDPAQLEAALAKLAPGQAAEQAAEQSS
                     EDAPPASPEGGAPADTTVH"
     gene            complement(725..1567)
                     /locus_tag="VHE37_14855"
     CDS             complement(725..1567)
                     /locus_tag="VHE37_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HVT36868.1"
                     /translation="MTEEIAAVDATNSEADKALPRVLGQPLDKLPEDLYIPPDALQVV
                     LEAFEGPLDLLLYLIRRQNLNILDIPVYRITQQYMEYIALMEKMRFELAAEYLVMAAL
                     LAEIKSRLLLPRPPPEIEEEGDPRMELVRRLQEYERFKTAGENIEAMPRMGRDLHVAQ
                     ARPEIVPTVAQIPTPSLRELLMAFKDAMSRADLFTHHHVSREPLSVRERMTHILARVR
                     NGPQQFFELVDPEEGRIGVVVVLLAVLELVKGAMVDLTQEAPFAPVTIAVKSAESEDE
                     NAHE"
     gene            complement(1564..2556)
                     /gene="trpS"
                     /locus_tag="VHE37_14860"
     CDS             complement(1564..2556)
                     /gene="trpS"
                     /locus_tag="VHE37_14860"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HVT36869.1"
                     /translation="MKPVVLSGIQPSGRLTIGNYLGALKNWVKLQEEFRCYYMLVDLH
                     AITVRQDPKVLRERCYEFLALYIACGLDPEKNTLFVQSHVPAHARLSWILNCYTQMGE
                     LNRMTQFKDKSARNTDNINAGLFDYPVLMAADILLYQTAQVPVGDDQKQHLELTRDVA
                     IRFNNVYGPVFKVPEPMIPPVGARIMGLQDPTAKMSKSGEAETDAIYLLDGPDVIVRK
                     LKRAVTDLGNEVRYDTAEKPGVSNLMSILAATTGMSFDAIAAQYNGKGYGAFKQAVAD
                     AVVEALKPVQEKYRHVRDDVDGLRRVLKRGAEQASAAGDATLMRVHEALGFIPE"
     gene            complement(2553..3221)
                     /locus_tag="VHE37_14865"
     CDS             complement(2553..3221)
                     /locus_tag="VHE37_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVT36870.1"
                     /translation="MPIELTPVQKIAVWALPILFALTLREIAHGWAAYALGDRTPMMF
                     GRLSLNPLNHADPIGTVVIPLVMILLGGFVFGWARPVPVSMRNFKHPRRGMALVAAAG
                     PLANFVMAFLWALLLRLALAQEASEGMWLGVQAMSLAGVSFNLVIAIINLLPIPPLDG
                     GRMVNGLLPSRLSMKYERLEPYGFFIVAIFLMTPMFNQLLYWPMALAQYFIFSLAGVS
                     AHTL"
     gene            complement(3392..4027)
                     /locus_tag="VHE37_14870"
     CDS             complement(3392..4027)
                     /locus_tag="VHE37_14870"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HVT36871.1"
                     /translation="MADWIQLHPVNPQQRLLAQVAVRLRQGAVIAYPTDSCYALGCHL
                     DDRDAADRLRRIRGFDRHHQFTLMCRDLSEIATYAHVDNQQFRLLKQLTPGPYTFILR
                     ATKELPKRVAHEKRKTIGIRVPDHVITQSLLDTLGEPLISCTLQFPGDDMPVCDPGEH
                     YAQLVRAVDVVLTGGDACGPEPTTVIDYSSESPRLVRQGRGDVSRLELAPV"
     gene            4068..4601
                     /locus_tag="VHE37_14875"
     CDS             4068..4601
                     /locus_tag="VHE37_14875"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HVT36872.1"
                     /translation="MNLLLNYGPAFAFLGAYLAGGIYLATEVLIASLILVVLLYRLWK
                     GVWHNMHMAVALIAAVLGGLTLYLHDPTFIKYKPSAVYGVFALALLTSQFVGDKVLLA
                     RIPQHTITLPDALWRKVNLAWALFFMFCAALNIYVAFHFDEATWVKFKAFGFTALMFV
                     FLLAHLPFVWRHLPQEP"
     gene            4598..4870
                     /locus_tag="VHE37_14880"
     CDS             4598..4870
                     /locus_tag="VHE37_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HVT36873.1"
                     /translation="MSADRLARMRAALLAAMPDAQIELIDDSAAHAGHAGAASGRGHY
                     RVQIVSQDFSGRKPIERHRLVYDALGAMMQTDIHALSISARTPGET"
     gene            complement(4871..5608)
                     /locus_tag="VHE37_14885"
     CDS             complement(4871..5608)
                     /locus_tag="VHE37_14885"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HVT36874.1"
                     /translation="MRAWLLTVATLAMVPAWAASPAGEVMLATGMATAKAPQGAVREL
                     HKGSEVFAGDLIASGPNSYLDMRFADGGYVLLRPETRFRIDAYAYTPPAEAADATPAP
                     RKRDQADDAAHPPSRALLRLLKGGLRAVSGSIGKQAGDEYQMDTPQATIGIRGTDYFA
                     VLCESDCVTDPTLVSALPPGSAPAGSLITGVYQGGIDVRARAVGVTAVNAGDYLVTLT
                     TGAQLKLPSLPRFLLVDPLPNPKNCAP"
     gene            complement(5608..>6007)
                     /locus_tag="VHE37_14890"
     CDS             complement(5608..>6007)
                     /locus_tag="VHE37_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36875.1"
                     /translation="FGVDTQLSKFPGTTYAFGSSAYNGNIYSAENGPVNMNSTGSGNE
                     SFVFLTSGSVNGQNNISTGGQVVVLDEPDDFQIPLFNQFQPAAEDEENTDTGTDKKNA
                     GGNAAKSARVRGPRGSVIRRTRLDNGKECR"
BASE COUNT          950 a         1994 c         1965 g         1098 t
ORIGIN      
        1 gctcctgcag ctgctagatc aatgcaccgt ggtgtccgcc ggcgcgccgc cctcgggcga
       61 cgccggcggc gcgtcttcgg acgattgctc ggctgcctgc tcagccgctt gtccgggcgc
      121 cagcttcgcc agcgcggctt ccagctgcgc cggatccttg atctccggca gcgacggcag
      181 gtcgtcgagc gacttcaggt tgaagtcatc gagaaactgc ggcgtggtgc cgaacaacga
      241 tggccggccc ggcacttcct tctggcccac ttcgcggatc cagccgcgct cgaccagcac
      301 gcgcacgatg ttgggcgaca gcgccacgcc gcgcacctct tcgatctcgc cgcgggtgat
      361 cggttggcgg tagcagacca gcgccagcgt ctccatcagc gcgcgcgaca gcttgggcgg
      421 cttctcctgg aacagcaggc tgacccattg cgcgtattcc ttgcgcacct ggatgcggta
      481 accgctgtgc acctcggtca gctcggccgc ccggccttcg agcgatcggg ccagcgcctc
      541 gaggccggcg cgcagttcgt cgcggcccac gcccagctcg ctgccgagca gccgctgcag
      601 gtgatccagc ggcagcggca actcggacgc gagcagcagc gcttcgagga tcttcggcag
      661 gtcgccggtg gtttccactg gcgccgccgc ttcgaccggc gcggccgcaa ccggctcttc
      721 gttgtcactc atgggcgttc tcgtcttcgg attctgcgga tttcacggcg atggtcacgg
      781 gcgcgaacgg cgcctcctgg gtcaggtcga ccatcgcgcc cttgaccagc tccagcactg
      841 cgagcagcac caccaccacg ccgatgcggc cttcctcggg atcgaccagc tcaaagaact
      901 gctgcggacc gttgcgcacc ctggccagga tatgcgtcat gcgctcgcgc actgacagcg
      961 gctcgcgcga cacatggtga tgggtgaaaa gatccgcgcg cgacatggcg tccttgaatg
     1021 ccatcagcag ttcgcgcagc gagggcgtcg ggatctgcgc caccgtgggc acgatttccg
     1081 ggcgcgcctg cgccacgtgc aggtcgcggc ccatgcgcgg catggcctcg atgttctcgc
     1141 cggcggtctt gaagcgctcg tactcctgca ggcggcgcac cagctccatg cgcggatcgc
     1201 cctcctcctc gatctccggc ggcggacgcg gcagcagcag gcgcgacttg atctcggcca
     1261 gcagcgcggc catcaccagg tattcggcgg ccagctcgaa gcgcatcttc tccatcagcg
     1321 cgatgtattc catgtactgc tgcgtgatgc ggtacacggg gatgtcgagg atgttcaggt
     1381 tctgccggcg gatcaggtac agcagcaggt cgagcgggcc ctcgaaggct tccagcacca
     1441 cctgcagtgc gtcgggcggg atgtagaggt cttccggcaa cttgtccagc ggctggccca
     1501 gcacccgggg cagcgccttg tctgcttcgg aattcgtagc atcaacggcg gcaatttctt
     1561 cggtcattcg gggatgaatc ccagggcttc gtgcacgcgc atcagcgtgg cgtcgccggc
     1621 ggcactggcc tgctcggcgc cgcgcttgag cacgcggcgc aggccgtcca catcgtcgcg
     1681 cacgtggcgg tatttttcct gcacgggttt cagcgcttcc accacggcgt cggccaccgc
     1741 ctgcttgaac gcgccgtagc ccttgccgtt gtactgcgcg gcgatggcgt cgaagctcat
     1801 gccggtggtg gcggcgagga tcgacatcag gttcgacacg cccggcttct ccgctgtgtc
     1861 gtagcgcacc tcgttgccca ggtcggtgac ggcgcgcttg agcttgcgca cgatgacgtc
     1921 ggggccgtcg agcaggtaga tggcgtcggt ctcggcctcg ccgctcttgc tcatcttcgc
     1981 ggtcgggtcc tgcaggccca tgatgcgcgc gcccaccggc gggatcatcg gctccggcac
     2041 cttgaacacc ggcccgtaaa cattgttgaa gcggatggcg acgtcgcgcg tcaattccag
     2101 gtgctgcttc tggtcgtcgc ccaccggcac ctgcgccgtc tggtacagca ggatgtcggc
     2161 ggccatcagc accgggtagt cgaacaggcc ggcgttgatg ttgtcggtgt tcctggctga
     2221 cttgtccttg aactgcgtca tgcgattcag ctcgcccatc tgcgtgtagc agttgaggat
     2281 ccagctcaag cgcgcatgcg ccggcacatg gctctgcacg aacagcgtgt tcttctccgg
     2341 gtcgagcccg caggcgatgt acagcgccag gaattcgtag cagcgttcac gcagcacctt
     2401 cgggtcctgc cgcacggtga tggcgtgcag gtccaccagc atgtagtagc agcggaattc
     2461 ttcctgcagc ttgacccagt tcttcagcgc gccgagatag ttgccgatgg tcaggcggcc
     2521 ggacggctgg atgccggaaa ggacgacagg tttcataagg tgtgcgcgga tacgccggcc
     2581 aggctgaaga tgaagtactg cgccagcgcc atcggccagt acagcaactg gttgaacatc
     2641 ggcgtcatca ggaagatggc gacgatgaag aatccgtagg gctcgagccg ctcgtatttc
     2701 atcgacagcc ggctcggcag caggccgttg accatgcgcc cgccgtcaag cggcggaatc
     2761 ggcagcagat tgatgatggc gatgaccagg ttgaacgaca cgccggccag cgacatggcc
     2821 tgcacgccca gccacatgcc ttcgctcgcc tcctgcgcca gcgccagcct gagcagcagc
     2881 gcccacagga aggccatgac gaaattggcc agcgggccgg ccgccgccac cagcgccatg
     2941 ccgcggcgcg gatgcttgaa gttgcgcatg gacaccggca ccggccgcgc ccagccgaag
     3001 acgaagccgc cgagcaggat catcaccagc gggatgacga cagtgccgat cggatcggcg
     3061 tggttcagcg ggttgagcga cagccgcccg aacatcatcg gcgtgcggtc gcccagcgcg
     3121 tacgcggccc agccatgcgc gatctcgcgc agcgtgaggg cgaacaggat gggcagagcc
     3181 cagaccgcga tcttctggac cggcgtgagt tcaatcggca tgcggcattt tagccgagcg
     3241 ggtctgccgc gtgcttcgcc gcgcgaattt tcccgctgga tttgcctgca ccgcactcca
     3301 ctttttgatg ttattaatta tcgtgtatta ttacttaact acaataatta ataacagcgg
     3361 aaatggccgt gcggttgagc ggttttggcg ctcacaccgg cgccagttca agccggctga
     3421 catcgcctct tccctggcgc accaaccgcg gactttccga cgagtagtcg atcaccgtgg
     3481 tcggctcggg gccgcaggcg tcaccgccgg tgagcaccac gtcgaccgcg cgcaccagct
     3541 gcgcatagtg ctcccccgga tcgcacaccg gcatgtcgtc gccgggaaac tgcagcgtgc
     3601 agctgatcag cggctcgccc agcgtgtcga gcagcgactg cgtgatgacg tggtccggca
     3661 cgcggatgcc gatcgtcttg cgcttctcgt gcgcaacgcg cttgggcagt tccttggtcg
     3721 cgcgcaggat gaaggtgtac gggcccggcg taagctgctt gagcaggcgg aactgctggt
     3781 tgtcgacatg cgcataggtg gcgatctcgg aaagatcgcg gcacatcagc gtgaactggt
     3841 gatgccggtc gaagccgcgg atgcggcgca ggcggtcggc ggcatcgcgg tcgtcgagat
     3901 ggcagccgag cgcgtagcac gagtcggtcg gatacgcgat gactgcaccc tggcgcagcc
     3961 gcaccgccac ctgcgccagc aggcgctgtt gcggattcac cgggtgcagc tggatccagt
     4021 cagccatcgc tcatcttaat cgccttgtgg cgacctataa tttcaggatg aatcttctgc
     4081 tcaattacgg acctgccttc gccttcctcg gcgcctatct cgccggcggc atctacctgg
     4141 ccactgaggt gctgatcgcc tcgctgatcc tggtcgtgct gctctaccgg ttgtggaaag
     4201 gcgtttggca caacatgcac atggcggtgg cgctgatcgc cgccgtgctc ggcggcctga
     4261 cgctctacct gcacgatccc accttcatca agtacaagcc cagcgccgtt tatggcgtgt
     4321 ttgcgctggc cttgctgacc agccagttcg tcggcgacaa ggtgctgctc gcgcgcattc
     4381 cgcagcacac catcaccctg cccgatgcgt tatggcgcaa ggtgaacctg gcctgggcat
     4441 tgttcttcat gttttgcgcg gcgttgaata tctacgtggc gtttcacttc gacgaagcca
     4501 cctgggtgaa gttcaaggcc ttcggcttca ccgcgctgat gttcgtgttc ctgctggcgc
     4561 acctgccctt cgtctggcgc cacctgccgc aggaaccatg agcgccgacc gcctcgcgcg
     4621 catgcgcgcc gcgttgctcg cggcaatgcc ggacgcgcag attgaattga tcgacgacag
     4681 cgccgcgcac gccggccatg ccggcgcggc gagcggacgc gggcattacc gcgtgcagat
     4741 cgtgtcgcag gatttttccg gcaggaaacc catcgagcgc catcgcctgg tgtacgacgc
     4801 gctcggcgcg atgatgcaga ccgacatcca cgcgctgtcg atcagcgcac gcacgccggg
     4861 cgagacctag ctagggcgcg cagttcttcg ggttaggcag cgggtccacc agcaggaagc
     4921 gcggcagcga cgggagcttc aactgcgcgc cagtggtcag cgtgaccagg taatcgcccg
     4981 cgttgaccgc cgtgacgccc accgcacgcg cacgcacatc gatgccgccc tggtagacgc
     5041 cggtgatcag gctgcctgca ggtgcactgc ccggcggcag cgccgacacc aacgtcggat
     5101 cggtcacgca atcgctctcg cacagcaccg cgaagtagtc ggtgccgcgt atgccgatcg
     5161 tggcctgcgg cgtgtccatc tggtactcat cgccggcctg cttgccgatg ctgcccgaca
     5221 ccgcgcgcag gccgcccttc agcaggcgca gcagcgcgcg cgaaggcgga tgcgccgcgt
     5281 catccgcctg gtcgcgcttg cgcggcgcag gcgtggcgtc cgccgcctcc gccggcgggg
     5341 tgtaggcgta agcgtcgatg cggaagcgcg tctccggccg cagcaacacg tagccgccgt
     5401 cggcgaagcg catgtcgaga tagctgttgg gacccgatgc gatcaggtcg ccggcaaaca
     5461 cttccgagcc cttgtgcagt tcgcggaccg cgccctgcgg cgccttggcg gtggccatgc
     5521 cggtcgccag catgacttcg ccggccggcg acgcggccca cgccggcacc atggcgagcg
     5581 ttgccaccgt cagcaaccac gcacgcatca gcggcactcc ttgccgttgt ccaggcgggt
     5641 gcggcggatc acgctgccgc gcggtccgcg cacgcgcgcg gatttcgcgg cgttgccgcc
     5701 ggcgtttttc ttgtcggtgc cggtgtccgt gttctcctcg tcctccgccg ccggctggaa
     5761 ctggttgaac agggggatct ggaaatcgtc gggctcatcc agaaccacca cctggccgcc
     5821 ggtactgatg ttgttctggc cgttgacgct gccggaggtg aggaagacga aactctcgtt
     5881 gcccgagccg gtcgagttca tgttgaccgg gccgttctct gcgctgtaga tgttgccgtt
     5941 gtacgcactc gaaccgaacg cgtaggtggt gccggggaat ttcgacagct gcgtgtcgac
     6001 gccgaag
//
LOCUS       DASZZC010000702         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_750241,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000702 DASZZC010000000
VERSION     DASZZC010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750241"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VHE37_14895"
     CDS             <1..626
                     /locus_tag="VHE37_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT36876.1"
                     /translation="RSVQGFLVQAVDRIIHVNVETVQPPPPGSDDGYLTAMTRYGEEL
                     VEIIDVEKVLAEVAGTPQAVSTAVLETVGERRQGRVLVADDSKVARAQIEKILQQIGV
                     ESVLVRDGREALRYLKDLAEHGKPVTEELVMVISDVEMPDMDGYTLTTEIRRDPRLKD
                     LYVLLHSSLSGVFNNAMVQKVGADRFIAKFSSDELAQGVIDRLKLVH"
     gene            697..1089
                     /gene="flgB"
                     /locus_tag="VHE37_14900"
     CDS             697..1089
                     /gene="flgB"
                     /locus_tag="VHE37_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HVT36877.1"
                     /translation="MSATDELFGIHAAALQVQSRRMELIASNIANADTPHYSARDIDF
                     AKALDQIANQAQLPAQATHPAHLPVADPASSLPVIERTPMQNSSDGNTVDVQTEQAQF
                     ADAALHYQASMNFVDARLRALLTAITGQ"
     gene            1104..1511
                     /gene="flgC"
                     /locus_tag="VHE37_14905"
     CDS             1104..1511
                     /gene="flgC"
                     /locus_tag="VHE37_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HVT36878.1"
                     /translation="MSTMKIFEIAGSALSAQSVRLNTVASNLANADAVGSDPNNVYKA
                     RMPVFQALSDPTTPDLASVKVTGVVENQAQAEKRYEPGHPLADKDGYVYAPNVNIVEE
                     MVNMISASRSYQGSVEVMNTAKDLALRTLALGK"
     gene            1515..>1963
                     /locus_tag="VHE37_14910"
     CDS             1515..>1963
                     /locus_tag="VHE37_14910"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HVT36879.1"
                     /translation="MTASVDPSTLSALGLAQQASSKTASQTLDQADFLKLMTTQLQYQ
                     DPMKPLDNAEFVSQMAQFSTVSGIQSLQAASLVGRDVLVPASSGYLADGGVLAGGAQV
                     SASGDVTVQIRDASGAVVRNLDLGTQPSGLATFVWDGKGDDGSTLPAG"
BASE COUNT          354 a          661 c          650 g          298 t
ORIGIN      
        1 accgctcggt gcagggtttc ctggtgcaag cggtggaccg catcatccac gtcaacgtgg
       61 agacggtgca gccgccgccg ccgggcagcg atgacggcta cctcaccgcg atgacccgct
      121 acggcgagga gctggtcgag atcatcgacg tggagaaggt gctggccgag gtggccggca
      181 cgccgcaggc ggtgtcgacc gccgtgctgg aaacggtggg cgaacggcgc cagggccgcg
      241 tgctggtggc cgacgattcc aaggtggcgc gcgcgcagat cgagaagatc ctgcagcaga
      301 tcggcgtgga gtcggtgctg gtgcgcgacg gccgcgaggc gctgcgctac ctcaaggacc
      361 tggccgagca cggcaagccg gtgaccgagg aactggtcat ggtcatctcc gacgtcgaga
      421 tgccggacat ggacggctac acgctgacga cggaaatccg gcgcgacccg cggttgaagg
      481 atctctacgt gctgctgcac agctcgctgt ccggcgtgtt caacaacgcc atggtgcaga
      541 aggtgggcgc cgaccgcttc atcgccaagt tctcctcgga cgagctggcc cagggcgtca
      601 tcgaccggct caagctggtc cactgatttt ttctccggcg gccgccgtgg cacacggctt
      661 gcctgactgc gctcatcgaa tctttgatga ggcgccatgt ccgcgactga cgagctgttc
      721 ggcatccacg ccgctgccct gcaagtgcag agccggcgca tggagctgat cgcctcgaac
      781 atcgccaacg ccgacacgcc acactacagc gcgcgcgaca tcgacttcgc caaggcgctg
      841 gaccagatcg ccaaccaggc gcagctgccg gcgcaggcca cccacccggc gcacttgccg
      901 gtggctgatc ccgcgtccag cctgccggtg atcgagcgca cgcccatgca gaacagcagc
      961 gacggcaata ccgtcgacgt gcagaccgag caggcgcagt tcgccgacgc cgcgctgcat
     1021 taccaggcca gcatgaattt cgtcgatgcg cggctgcgcg cattgctgac cgcgatcacc
     1081 gggcaatagc cggagccctc gccatgtcga ccatgaagat tttcgagatt gccggcagtg
     1141 cgctgtcggc gcagtcggtg cgcctgaaca cggtggcctc caacctggcc aacgccgatg
     1201 cggtgggcag cgatccgaac aatgtctaca aggcgcgcat gccggtgttc caggcgctgt
     1261 ccgaccccac cacgcccgat ctcgcctcgg tgaaagtgac cggcgtggtc gagaaccagg
     1321 cgcaggcgga gaagcgctac gagccgggac atccgctggc cgacaaggac ggctacgtct
     1381 atgcgcccaa cgtcaacatc gtcgaggaga tggtcaacat gatctcggcc tcgcgttcct
     1441 atcaaggcag cgtcgaagtg atgaacacgg ccaaggacct ggcgctgcgc actcttgccc
     1501 tcgggaaata agccatgacc gcatctgttg atcccagcac cttgtcagcg ctcggcctgg
     1561 cgcagcaggc ctccagcaag accgcgtcgc agacgctgga ccaggcggat ttcctcaagc
     1621 tgatgaccac gcagctgcag taccaggacc cgatgaagcc gctggacaac gccgagttcg
     1681 tcagccagat ggcgcagttc tccaccgtgt cgggcatcca gtcgctgcag gccgcctcgc
     1741 tggtcggccg cgacgtgctg gtgccggcca gctccggcta ccttgccgat ggcggcgtgc
     1801 tcgccggcgg cgcgcaggta agcgccagcg gcgatgtcac cgtgcagatc cgggacgcca
     1861 gcggcgcggt ggtgcgcaac ctcgacctcg gcacgcagcc cagcggcctc gccacctttg
     1921 tctgggacgg caagggcgac gacggcagca cgctgcccgc cgg
//
LOCUS       DASZZC010000703         7057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_430711,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000703 DASZZC010000000
VERSION     DASZZC010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430711"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VHE37_14915"
     CDS             complement(<1..315)
                     /locus_tag="VHE37_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36880.1"
                     /translation="MKTFMGISGALLLAASCGASAQSNWYDPHPGKISYSYIEGGYLR
                     GKDLVDRSTGLSQDADGFDVGASFALGAPAFIFGDYRSMDSGEFSALVGGPPPTQQSG
                     KIR"
     gene            449..1195
                     /locus_tag="VHE37_14920"
     CDS             449..1195
                     /locus_tag="VHE37_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36881.1"
                     /translation="MNTRRWLLALACMLCTDAGAADFSLGADYWGYQVRGSVNRNGDV
                     LDFDRDLGVQAQTHALLQAAWNTGPGWWKPDFALSYAPIKAGGQQEVTAGTRFGGLLF
                     PSGNTVIFGDADLKDLALTLRYPLALGNARIWGGISAKHVNGEVTVRDRNDTQEDVQD
                     INQWFPMLHVAAAIPAASWLTLSAEANGARYASNQAWDVRAGASIGIYGPLGLNAGWA
                     ARHYHVESDSSYRLDATLSGVTAGLYLLLR"
     gene            complement(1214..1549)
                     /locus_tag="VHE37_14925"
     CDS             complement(1214..1549)
                     /locus_tag="VHE37_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT36882.1"
                     /translation="MKRIALMLLLAAAAPAFAQDAAVPKAAATCFSCHGENGAHPIAP
                     NYPVLAGQYANYLEHALTEYKEGKRKNPIMGAQAAALSGEDIHQLAEFFSEQPSNLHT
                     PSVHVAEKK"
     gene            complement(1559..1882)
                     /locus_tag="VHE37_14930"
     CDS             complement(1559..1882)
                     /locus_tag="VHE37_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT36883.1"
                     /translation="MKFARWGVFALAAAVAVPALADGDAAAGRIKANTCMGCHGIPNY
                     NNVYPTYRVPRIGGQSAGYITAALTAYKNGERPHKTMQAQAASLSDQDIADIAAYIAS
                     APAHP"
     gene            2130..3647
                     /locus_tag="VHE37_14935"
     CDS             2130..3647
                     /locus_tag="VHE37_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HVT36884.1"
                     /translation="MSAVVLPAPQQGAEVAAVDLGSNSFHMMVARGGADGLQVIDRLR
                     ESVRLAAGLTADRRLDPEMQARALACLQRFGQRLMHLPPERVRVVGTNTLRKLKKGAA
                     FIAAAEAALGHQIEIISGAEEARLVYGGVIQGMGSEKPRRLVVDIGGGSTEIIIGKGR
                     QPRLVESVSLGCVTHHQLYFGSGEITAQNFARARLAARVQLEFLERRYRRAGWDLAIG
                     ASGTVRGVWRVLMAEGWSRDEITREGLEKLVELVLQRGHVNRIDFPALREDRRPVFAG
                     GLAVLVGVFDALQIERMQTSEHALREGVIYDLLARLGSGDIRGESALAMARRYHADLR
                     QAAQIERTTQKLLDQVAADWELDRKSGAKLLGWATQLHEIGLVISHNSYHKHGEYILR
                     NCDLAGFSQTEQFILAALVRLHRGKFTPSALDDVPETWRETVQRLAILLRLAALLHRS
                     RVPHAQLPIKLAAGRKSLDVSFTRRNWLSRHPLTRADLEREAEYLKAIEVRLKLD"
     gene            3689..4225
                     /locus_tag="VHE37_14940"
     CDS             3689..4225
                     /locus_tag="VHE37_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVT36885.1"
                     /translation="MRQIAALLLLTAAATAVAADSKPADGSDKVYRYTDDNGVVHYTD
                     KPPSKDARPVDLPPLQTYRFKKPPRTAQAPPPPPAAPTFELAITSPTPDQTLRSGTDI
                     AVSVSVMPGLLDGYGLIYSLDGAAQTADAIPDTSYTLKGANRGDHTVTVSLVSPDKQE
                     LTSTSITVHMLPPTVNKP"
     gene            4307..5383
                     /gene="glnL"
                     /locus_tag="VHE37_14945"
     CDS             4307..5383
                     /gene="glnL"
                     /locus_tag="VHE37_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HVT36886.1"
                     /translation="MSLLARARVKPDAVLDGLTTAVVALDERLRVVSLNSACESLFGI
                     SRRHALSAPLVEGIPHFAAVEPRLREALAAGTGFIERELQLKKNGDGAITVDCTATPM
                     LAGKERGLLLEVLPLDRHLRISRDELLLAQHQASREMIRGLAHEIKNPLGGIRGAAQL
                     LEREFPDVQHREYTRVIIREADRLQNLVNRLLGPNRAPRKEPLNVHEVLEHVRQLVQA
                     EAADGVAFLRDYDPSIPDLVADREQLIQAALNVVRNALQAIGSEGTIVLRSRTRRQIT
                     IAGARHKLVIQLDVEDDGPGIAPDMIEKIFYPMVSTRAGGSGLGLPIAQYLIHSHGGL
                     IECRSKPGNTVFSMYLPLDIQDTR"
     gene            5380..6819
                     /gene="ntrC"
                     /locus_tag="VHE37_14950"
     CDS             5380..6819
                     /gene="ntrC"
                     /locus_tag="VHE37_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HVT36887.1"
                     /translation="MNTHLTVWVVDDDESIRWVLEKSLARDGMRVKTFPGAAELFDAL
                     SDATPDVLISDIRMPGVSGLELMERVRKSKPELPVIIMTAHSDLDAAVASYKGGAFEY
                     LPKPFDVDGVATLVRRAARKREAPEDTPAVPGKTIIGEAPAMQDLFRAIGRLSQSQIT
                     VLITGESGTGKELVARALHQTSPRAQKPFIAINTAAIPKDLLESEFFGHERGSFTGAQ
                     TQRKGRFEQADGGTLFLDEIGDMPADLQTRLLRVLQDGEFYRVGGVAPIKVDVRVIAA
                     THQELEKLVTAGRFREDLYHRLNVIRVRIPALRERAADIPQLARHFLAAAARELNTES
                     KRLRPEALQQLKAYEWPGNVRQLENTCRWLTVMAPGRDVHLADLPPELRAGAPVVAAP
                     VTIAAPQAAIAAVSVASGEWHELLRVWADRALAAGNSDLLGTATPLFERVMIEAALKE
                     TGGQRQEAAKRLGWGRNTLTRKIKELGME"
BASE COUNT         1235 a         2385 c         2325 g         1112 t
ORIGIN      
        1 gcgtatcttg ccactctgct gcgtcggcgg cgggccgccg accagcgccg aaaattcccc
       61 ggaatccatg ctgcggtagt cgccgaaaat gaatgcgggc gcgccgagcg cgaaggacgc
      121 gccgacatcg aagccatcgg cgtcctggct gagaccggtg gaccggtcca ccagatcctt
      181 gccacggaga tagccaccct cgatgtaact gtaggaaatc ttgccggggt gcgggtcgta
      241 ccagttgctc tgggcgctcg caccgcagga tgcggcgaga agcaaagcgc cggaaatgcc
      301 catgaacgtc ttcatacagc ctccttactt ccgaagtgcg cgggtatggt tgttctcgcg
      361 ctgtcgccgg attggagcct gcggcctgcg gcaaagttct gtttcgcgca cgcggcgtcg
      421 ggccgccgga ttcggctaag ctggccgcat gaacactcgc cgctggctgc tcgcactcgc
      481 ctgcatgctg tgcaccgacg ccggtgctgc cgatttttcc ctcggcgccg attactgggg
      541 ctaccaggtc cgcggcagcg tcaatcgcaa cggcgacgtg ctcgatttcg atcgcgacct
      601 cggcgtgcag gcacagaccc acgcgctgct gcaggcggcg tggaacaccg gccccggctg
      661 gtggaaaccc gatttcgcgc tgtcctatgc gccgatcaag gccggcggcc agcaggaagt
      721 gacggccggc acgcgcttcg gcggcctgtt gtttccgtcc ggcaacacgg tgatcttcgg
      781 cgatgccgac ctgaaggacc tggcgctcac gctgcgctat ccgctggcgc tgggcaacgc
      841 gcgcatctgg ggcggcatca gcgccaagca cgtcaatggc gaagtgaccg tgcgcgaccg
      901 caacgacacg caggaagacg tgcaggacat caaccagtgg tttcccatgc tgcacgtggc
      961 cgccgcgatt ccggcggcct catggctgac gctcagcgcg gaagccaatg gcgcgcgcta
     1021 cgccagcaac caggcatggg atgtgcgcgc gggcgccagc atcggcattt acgggccgct
     1081 gggcctcaat gccggctggg cggcgcgcca ctaccacgtg gaatcggaca gcagctatcg
     1141 gctcgacgcc acgctgtccg gcgtcaccgc cgggctttac ctgctgcttc gctgaacgca
     1201 gcgggacgcg gaactacttc ttctcggcga cgtgcacgct gggcgtgtgc aggttggacg
     1261 gctgctcgct gaagaattcg gccagttggt gaatgtcttc ccccgacaag gccgcggcct
     1321 gcgcgcccat gatcgggttc ttgcgcttgc cttccttgta ttccgtgagc gcgtgctcaa
     1381 ggtagttcgc atactgcccg gcgagcaccg ggtagttcgg cgcaatcgga tgcgcgccgt
     1441 tctcgccgtg gcagctgaag caggtggccg cggccttggg cacagcggcg tcctgcgcaa
     1501 acgccggcgc ggcagcggcc agcaacagca tcaatgcaat gcgcttcatg aaatcccctt
     1561 atggatgcgc cggcgccgag gcgatgtacg cggcgatgtc agcgatgtcc tggtcggaca
     1621 ggctggcagc ctgcgcctgc atggtcttgt gcgggcgctc gccgttcttg tacgcggtca
     1681 gcgcagcggt gatgtaaccg gcgctctgtc cgccgatgcg cggcacgcgg taggtcggat
     1741 agacattgtt gtagttcggg atgccatggc aacccatgca ggtgttggcc ttgatgcggc
     1801 cagcggcagc gtcgccatcg gcaagcgcgg gcacggcaac agcagcggca agggcgaaga
     1861 cgccccaacg cgcgaatttc ataaaagcaa ctcccgggaa aagcgagtgg agccaaaaag
     1921 gcgcgctatg ttcgggcaat tgccattcgc ggtcaacgaa atgtcgtgcg actgccatgc
     1981 accggcgcaa agctgcgcga agacagcttc accgcacccc aggttacgct gttattaatg
     2041 ctttattgaa gtcaaaattt agcgataata gataacatca tgcacatgcc tgcggttgac
     2101 tcgatttgca ccggtgcgag gtcgctcgca tgagcgcggt cgtgttgccg gcgccgcagc
     2161 agggtgcgga agtggccgcg gttgacttgg gctccaacag ttttcacatg atggtcgcgc
     2221 gcggcggcgc agatggcctg caggtgatcg accgcctgcg cgaaagcgtg cgcctggctg
     2281 cggggctcac cgcggacagg cggctcgatc cagaaatgca ggcgcgcgcg ctggcctgcc
     2341 tgcagcgctt cggccagcgc ctgatgcacc tgccgccgga acgcgtgcgc gtggtcggca
     2401 ccaacacgct gcgcaagctg aagaagggcg ccgccttcat cgccgcggcc gaggccgctc
     2461 tcggacacca gatcgaaatc atttccggcg ccgaggaagc gcgcctggtc tacggcggcg
     2521 tgatccaggg catgggcagc gagaagccgc ggcgcctggt ggtcgacatc ggcggcggca
     2581 gcaccgagat catcatcggc aaggggcgcc aacccaggct ggtggaatcg gtttcgctgg
     2641 gctgcgtcac ccatcaccag ctgtatttcg gcagcggcga gatcaccgcg cagaattttg
     2701 cacgcgcgcg gctggcggcg cgcgtgcaac tggaattcct ggagcggcgc taccgccgcg
     2761 ccggctggga tctggccatc ggcgcgtcag gcacggtgcg cggcgtgtgg cgcgtgctga
     2821 tggccgaggg ctggagccgc gacgagatca cgcgcgaggg cctggagaag ctggtcgaac
     2881 tggtgctcca gcgcggtcac gtcaaccgca tcgattttcc cgcgctgcgc gaggaccggc
     2941 gcccggtctt tgccggcggc ctcgcggtgc tggtcggcgt gttcgatgcg ctgcagatcg
     3001 agcgcatgca gacctccgag cacgcgctgc gcgagggcgt gatctacgac ctgctcgcgc
     3061 gtctgggcag cggcgacatc cgcggcgaat cagcgctggc catggcgcgg cgctaccacg
     3121 ccgacctgcg ccaggccgcg cagatcgagc gcaccaccca aaagctgctg gatcaggttg
     3181 ccgcggactg ggaactggat cgcaagagcg gcgccaagct gctcggctgg gccacgcagc
     3241 tgcacgagat cggccttgtc atctcgcaca attcgtatca caagcacggc gaatacatcc
     3301 tgcgcaattg cgacctggcc ggcttctcgc agaccgagca gttcattctc gcggcgctgg
     3361 tgcggctgca ccgcggcaag ttcacgccgt cggcgctgga cgatgtgccc gagacctggc
     3421 gcgagaccgt gcagcgcctg gccatcctcc tgcggctggc ggcgctgctg catcgctcgc
     3481 gcgtgccgca cgcgcaactg ccgatcaagc tcgccgccgg tcgcaagtcg ctggacgttt
     3541 ccttcacgcg ccgcaactgg ctgtcgcggc atccgctgac ccgcgccgac ctcgagcgcg
     3601 aggcggaata cctcaaggcc atcgaagtac ggttaaagct cgattaagca gcggcgacta
     3661 caatgccgcc agaccacgga aacggacgat gcgccagatt gctgccctgc tgctgttgac
     3721 cgccgccgcg actgccgtcg ccgccgacag caagcctgcc gacggttccg acaaggtgta
     3781 ccgctatacc gacgacaacg gcgtggtgca ctacaccgac aagccgccga gcaaggacgc
     3841 caggccggtc gacttaccgc cgctgcagac ctaccgcttc aagaagccgc cgcgcacggc
     3901 gcaggctccg ccgccgcctc cggctgcgcc aaccttcgaa ctggccatca cctcgcccac
     3961 gccggaccag accctgcgca gcggcaccga catcgccgtt tcggtgagcg tgatgcccgg
     4021 attgctcgat ggatacggcc tgatctattc cctcgacggc gcagcgcaga ccgccgacgc
     4081 gattcccgac acgagctaca cgctcaaggg cgccaaccgc ggcgatcaca ccgtcacggt
     4141 gtcactggtc agcccggaca agcaggaact gaccagcacc agcatcaccg tgcacatgct
     4201 gccgcccacg gtgaacaaac cctgatccgg cggcgcaccg atttggtgcg ccaaaccgat
     4261 cgatgcgcta cgctggcgct ggcgtactcc ttgctttcca gcggccatgt ccctgcttgc
     4321 ccgggcgcgg gtcaaacccg acgccgtcct cgatggcctg accaccgccg tggtggcgct
     4381 cgacgagcgc ctgcgcgtgg tctcgctcaa ttcagcctgc gaaagcctgt tcggcatctc
     4441 gcgccggcat gcgctcagcg caccgctggt ggaaggcatc ccgcactttg cggcggtcga
     4501 accgcgactg cgcgaggctc tggccgccgg cactggattc atcgagcgcg aactgcagtt
     4561 gaagaaaaac ggcgacggcg ccatcaccgt cgattgcacc gccacgccga tgctggccgg
     4621 caaggaacgc ggcctgctgc tggaagtgct gccgctggac cgccacctgc gcatctcgcg
     4681 cgacgaactg ctgctggcgc agcaccaggc cagccgcgag atgatccgcg gcctggcgca
     4741 tgaaataaaa aacccgctgg gcggcatccg cggcgcggcg caattgctgg aacgcgaatt
     4801 tcccgacgtg cagcaccgcg aatacacgcg cgtgatcatc cgcgaagcgg accgcctgca
     4861 gaacctggtc aaccgcctgc tcggccccaa ccgcgcgccg cgcaaggagc cgctcaacgt
     4921 gcatgaagtg ctcgaacacg tgcgccagct ggtgcaggct gaggccgccg atggcgtcgc
     4981 cttcctgcgc gactacgatc cttccattcc cgacctggtc gccgaccgcg agcagctgat
     5041 ccaggcggcg ctcaacgtgg tgcgcaacgc gctgcaggcc atcggcagcg aaggcaccat
     5101 cgtgctgcgt tcgcgcacgc gacggcagat caccatagcc ggcgcccgcc acaagctggt
     5161 catccagctc gacgtcgagg acgacggccc cggcatcgcc ccggacatga tcgagaagat
     5221 cttctacccg atggtcagca cccgcgccgg cggcagcgga cttggcctgc cgattgcaca
     5281 gtacctgatc cacagccacg gggggctgat cgaatgccgc agcaagccgg ggaacaccgt
     5341 gttctccatg tacctgccgc tggacatcca agacacgcga tgaacacgca tctgaccgta
     5401 tgggtggtcg acgacgacga gtcgatccgc tgggtgctgg agaaatccct ggcgcgcgac
     5461 ggcatgcgcg tgaagacttt tcccggtgcc gcggaattgt tcgacgcgct gtccgacgcc
     5521 acgcccgacg tgctgatctc cgacatccgc atgcccggag tgtccggcct cgaactgatg
     5581 gagcgcgtgc gcaagagcaa gcccgagctg ccggtgatca ttatgaccgc gcactccgac
     5641 ctcgacgccg cggtggcgtc gtacaagggc ggcgcgttcg agtacctgcc caaacccttt
     5701 gacgtcgacg gcgtcgccac gctggtgcgg cgcgctgcca ggaagcgcga agcaccggaa
     5761 gacacgccgg cggtgccggg caagaccatc atcggcgagg cgccggcgat gcaggacctg
     5821 ttccgcgcca tcggccgact gtcgcagtcg cagatcaccg tgctgatcac cggcgagtcc
     5881 ggcaccggca aggaactggt ggcgcgcgcg ctgcaccaga ccagcccgcg ggcgcaaaag
     5941 ccgttcatcg ccatcaacac cgcagcgatt ccgaaggatc tgctggagag cgaattcttc
     6001 ggccacgagc gcggctcgtt caccggcgcg cagacgcagc gcaagggccg cttcgagcag
     6061 gccgatggcg gcacgctgtt cctcgacgag atcggcgaca tgccggccga tctgcagacg
     6121 cgcctgctgc gcgtgctgca ggacggcgag ttctaccgcg tcggcggcgt cgcgccgatc
     6181 aaggtcgatg tgcgcgtgat cgccgccacg catcaggagc tggaaaagct ggtgaccgcg
     6241 ggccgcttcc gcgaagacct gtatcaccgc ctcaacgtga tccgcgtgcg catccccgcg
     6301 ctgcgcgaac gtgcggccga tattccgcag ctggcccggc attttctcgc tgccgcggcg
     6361 cgcgagctca acaccgaatc caagcgcctg cgcccggaag cgctgcagca gctcaaggcc
     6421 tacgagtggc cgggcaacgt gcggcaactg gaaaacacct gccgctggct cacggtgatg
     6481 gcaccggggc gcgatgtgca cctggccgat ctgccaccgg agttgcgcgc gggtgccccg
     6541 gtcgtggccg caccggtgac gatcgctgcg ccgcaggctg cgatcgcggc agtaagtgtc
     6601 gccagcggcg aatggcacga actgctgcgc gtctgggccg accgcgcgct ggccgccggc
     6661 aacagcgacc tgctcggcac ggccacgccg ctgttcgaac gcgtgatgat cgaagccgca
     6721 ctgaaggaaa ccggcggcca gcgtcaggaa gccgccaagc ggctgggctg gggccgcaat
     6781 acgctcacgc ggaaaatcaa ggaactcggg atggagtgaa ccggcgtaat tcaatccggg
     6841 gagttgcagc cctgggcgtc cactcgcatt tcctccgcat ccccgcaagc ggggcccctg
     6901 ctcgggctcg gaacgccggc cttcatacgc tcacgcggaa aatcaaggaa ctcgggatgg
     6961 agtgaaccgg cgtaattcaa tccggggagt tgcagccctg ggcgtccact cgcatttcct
     7021 ccgcatcccc gcaagcgggg cccctgctcg ggctcgg
//
LOCUS       DASZZC010000704         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1081463,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000704 DASZZC010000000
VERSION     DASZZC010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081463"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /locus_tag="VHE37_14955"
     CDS             <1..1732
                     /locus_tag="VHE37_14955"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HVT36888.1"
                     /translation="ETAADDEVVFQHLSECRPLARMSWRELARQVRIVATQLRALGVV
                     PGDRVVSYMPNVPETAVAMMATVAIGAVWSSAAPEFGVKTVIERFSQIAPKVLFAADG
                     YRFGGKDFAREAEVRRIVADLPTLQHVVWLGYLKPAAPPPDLRGLLNWSQLVSYADVP
                     RGKFRFERVAHDHPLWVLFSSGTTGLPKAIVHSHVGMLLEHLKLMHFHLNLRRGSVMF
                     FYSTTGWVMWNILLSALTAGAGVVLYDGSPVHPDPGLLWKLAADTRATNFGASPTFVQ
                     MMEKARLKPGETHDLSALEAVLVSGAPSTPETFDWFYRCVKRDLWVTSQSGGTEIGSG
                     FVGASPTLPVHAGEIQTRMLGMDVHAWDDDGNEVIDKVGELVVTTPFPSMPIHFWNDE
                     DGKRYRESYFDTYPGVWRHGDFIKVNARGGCYIYGRSDSTLNRHGVRIGTAEIYRAVE
                     QLPEIADSLIVCCELPGGQFFMPLFIKLRPGHALDAALQARMYARLREDCSPRHVPDR
                     TYAVHEIPYTLTGKKMEIPVRKILMGWPLEKAASRDAMANPDAIDWFVRFAQESIDYQ
                     WRKPEAALAT"
     gene            1746..2861
                     /locus_tag="VHE37_14960"
     CDS             1746..2861
                     /locus_tag="VHE37_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HVT36889.1"
                     /translation="MSSKKPVVIYGVNGYTGRLVAEYLREYRIPFIAAGRDAKRVKDV
                     IDKVPGIENAEYEIVQVDHTREALTKLFKGARVVCNMVGPFMKYGPEAVEASLAAGCH
                     YTDTNGEQNWMMHAESEWGARFAERGLLLAPGIAQMYTTGEIAANICLEKPGVDTLDI
                     LVLWKGFPTYASTQTIFSILTANFYYLEQKQYVQWSPFAKAEVMVPGQHAVAMALPWG
                     GTAHPVWFKNDPRVANCRAMGGVFERTVMEGVVQITSVVEDKIKKLPKAEQEKALSDM
                     AATLQAGMPPRENQRINTSLDSVHASGPRGAHVHCVIHGSCNYKQTGLLQAYAAYHLL
                     QEKPLRTGFASGCQAFGYKALLGALQSFGLVGEPVVT"
     gene            2890..3912
                     /locus_tag="VHE37_14965"
     CDS             2890..3912
                     /locus_tag="VHE37_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVT36890.1"
                     /translation="MNTTNRRIVLASRPVGEPTPADFRLEQQPVPEPGDGEVLLRTLY
                     LSLDPYMRGRMSDAPSYAAPVAINAVMCGATVCRVENSRAEKFRTGDLVLAYSGWQDY
                     AVSPAKGLVKLDAQGVPPSWALGILGMPGMTAYVGLLDIGQPKAGETVVVAAATGPVG
                     SAVGQIARLKGCRAIGIAGGTEKCDYAVRELGFDLCLDHYRDDLPQQLAAACPQGIDV
                     YFENVGGHVLQAVVPLLNVNARMPLCGVIAWYNLDRLSAGPDFTPVLMRRLLAQRVKL
                     QGFIVSDHGARLADFQRDMAGWLREGKVRYREDVVEGLEHAPGAFIGLLKGSNFGKLV
                     IRVSPG"
     gene            3993..>4946
                     /locus_tag="VHE37_14970"
     CDS             3993..>4946
                     /locus_tag="VHE37_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT36891.1"
                     /translation="MSTVRTGSAKAPIEVYTHKFFTPPAYAGVIRRDQLIERIFQHSA
                     RVVLLQGPAGHGKSTALQQLKAVCDERGQVTGWLTLDDADNDTRRFYLHVHALLKSLK
                     LSDRKGEGHDGGRRRSDWLLERMQRAGKPVAIFFDDFQTLTNRSLLMFFRELFERLPD
                     NVRFYIGTRSTPELGLPRLVVNHRALILHADDLRFSPAEVERFFAGGPRVQISAEEIA
                     AIYRRTEGWPAALQLFRLSLASPDVRRSLDDLTSSRPRELAEYLADNVLALQPPDMRD
                     FLLRTALLPRLTASLCHAVTGRVDAQELLLRLERSGLFVRSL"
BASE COUNT          825 a         1674 c         1667 g          780 t
ORIGIN      
        1 ggaaaccgcg gccgacgacg aggtggtgtt ccagcacctg tcggagtgcc gccccctggc
       61 gcgcatgagc tggcgcgagc tggcgcggca ggtgcgcatc gtcgccacgc agctgcgcgc
      121 gctcggcgtc gtccccggcg accgcgtggt cagctacatg cccaacgtgc cggagaccgc
      181 ggtggcgatg atggccacgg tggccatcgg cgcggtgtgg tcgagcgcgg cgcccgagtt
      241 cggtgtcaag accgtgatcg agcgcttctc gcagatcgcg ccgaaggtgc tgttcgccgc
      301 cgatggctac cgcttcggcg gcaaggactt tgcgcgcgag gccgaggtgc ggcgcatcgt
      361 cgccgacctg ccgacgctgc agcacgtggt atggctgggt tacctgaagc cggccgcgcc
      421 gccgccggat ctgcgcggcc tgctcaactg gtcgcagctg gtgtcgtacg ccgacgtgcc
      481 gcgcgggaaa ttccgcttcg aacgcgtggc gcacgatcac ccgctgtggg tgctgttctc
      541 ctccggcacc acgggcctgc ccaaggccat cgtgcattcg cacgtgggca tgctgctcga
      601 gcatctcaag ctgatgcact ttcacctgaa cctgcgccgc ggcagcgtga tgttcttcta
      661 cagcaccacc ggctgggtca tgtggaacat cctgctgtcg gcgctcaccg ccggcgccgg
      721 cgtggtgctg tacgacggca gcccggtgca tcccgatccc ggcctgctgt ggaagctggc
      781 cgccgacacg cgcgccacca atttcggcgc cagccccacc ttcgtgcaga tgatggagaa
      841 ggcgcggctc aagcccggcg agacgcatga cctgtccgcg ctggaagcgg tgctggtgtc
      901 gggcgcgccg agcacgccgg agaccttcga ctggttctac cgctgcgtga agcgcgacct
      961 gtgggtgacc tcgcagtcgg gcggcaccga gatcggcagc ggcttcgtcg gcgcctcgcc
     1021 cacgctgccg gtgcacgccg gcgaaatcca gacgcgcatg ctcggcatgg acgtgcatgc
     1081 ctgggacgac gacggcaacg aggtcatcga caaggtcggc gaactggtgg tgaccacgcc
     1141 atttccgtcg atgccgatcc atttctggaa cgacgaggac ggcaagcgct accgcgaaag
     1201 ctatttcgac acctatcccg gtgtgtggcg ccacggcgac ttcatcaagg tgaacgcgcg
     1261 cggcggctgc tacatttacg gccgttccga ctccacgctc aatcgccacg gcgtgcgcat
     1321 cggcaccgcg gaaatctacc gcgcggtgga gcagctgccg gagatcgccg actcgctgat
     1381 cgtctgctgc gagctgcccg gcggccagtt cttcatgccg ctgttcatca agctcaggcc
     1441 cggccacgcg ctcgacgccg cgctgcaggc gcgcatgtac gcgcggctgc gcgaggactg
     1501 ctcgccgcgc cacgtgcccg accgcaccta cgccgtgcat gaaattccgt acacgctcac
     1561 gggcaagaag atggagatcc cggtgcgcaa gatcctgatg ggctggccgc tggagaaggc
     1621 ggcgagccgc gacgccatgg ccaatcccga tgccatcgac tggttcgtgc gcttcgccca
     1681 ggaatcgatc gactaccagt ggcgcaagcc ggaagccgcg ctggccacct aacgaggaga
     1741 gagcaatgag cagcaagaag cccgtggtga tttacggcgt caacggctac accggccgcc
     1801 tggtggcgga gtacctgcgc gagtaccgca tcccgttcat cgccgccggc cgcgatgcca
     1861 agcgggtgaa ggacgtgatc gacaaggtgc ccggcatcga gaacgccgag tacgaaatcg
     1921 tgcaggtgga tcacacgcgc gaagcgctga ccaagctgtt caagggcgcc agggtggtgt
     1981 gcaacatggt gggaccgttc atgaagtacg gaccggaggc ggtggaggcc agcctcgccg
     2041 ccggctgcca ctacaccgac accaacggcg agcagaactg gatgatgcac gccgaaagcg
     2101 agtggggcgc caggttcgcc gagcgcggcc tgctgctggc gccgggcatc gcgcagatgt
     2161 acaccacggg ggaaatcgcc gcgaatatct gcctggaaaa acccggcgtc gacacgctcg
     2221 acatcctggt cctgtggaag ggctttccca cttacgcgtc gacgcagacg attttttcca
     2281 tcctcaccgc caacttctat tacctggagc agaagcagta cgtgcagtgg tcgccattcg
     2341 ccaaggccga ggtgatggtg ccgggccagc acgcggtggc catggcgctg ccctggggcg
     2401 gcaccgcgca cccggtatgg ttcaagaacg atccgcgcgt ggccaactgc cgcgccatgg
     2461 gcggcgtgtt cgagcgcacg gtgatggaag gcgtggtgca gatcaccagc gtggtcgagg
     2521 acaagatcaa gaaattgccg aaggccgagc aggagaaggc gctgtcggac atggccgcta
     2581 cgctgcaggc cggcatgccg ccgcgcgaga accagcgcat caacacctcg ctcgacagcg
     2641 tgcatgccag cggcccgcgc ggcgcgcacg tacattgcgt gatccatggc agctgcaact
     2701 acaagcagac cggtctgctg caggcctatg ccgcgtatca cctgctgcag gaaaagccgc
     2761 tgcgcacggg cttcgcttcc ggctgccagg cattcggcta caaggcgctg cttggcgcgt
     2821 tgcagagttt cgggctggtg ggcgaaccgg tggtgacgta agcgacacgc gcggacgcac
     2881 aatgcgcgca tgaacacgac caaccgccgc atcgtcctcg ccagccggcc ggtcggcgag
     2941 ccgacacccg cggacttccg cctcgagcaa caaccggttc cggaaccggg agacggcgag
     3001 gtgctgctgc gcacgctgta cctgtcgctc gatccataca tgcgcggccg catgagcgac
     3061 gcgccgtcgt acgcagcgcc ggtggcgatc aatgccgtga tgtgcggcgc caccgtgtgc
     3121 cgcgtggaaa attcgcgcgc ggagaaattc cgcaccggcg acctcgtgct ggcctacagc
     3181 ggctggcagg actacgcagt gtcgccggcc aaggggctgg tgaagctcga cgcgcagggc
     3241 gtgccgccgt cgtgggcgct gggcatcctg ggcatgccgg gcatgaccgc gtatgtcgga
     3301 ttgctcgaca tcggccagcc gaaggccggc gaaacggtgg tggtcgccgc agccaccggc
     3361 ccggtcggtt cggcggtcgg gcagatcgcc aggttgaaag gctgccgcgc gatcggcatc
     3421 gccgggggca cggagaaatg cgactacgcc gtccgcgaac tcggcttcga tctctgcctg
     3481 gatcactacc gcgacgatct gccgcaacag ctggccgcgg cctgcccgca aggcatcgac
     3541 gtgtacttcg agaacgtcgg cggccacgtg ctgcaggcgg tggtgccgct gctcaacgtc
     3601 aacgcgcgca tgccgttgtg cggcgtcatc gcctggtaca acctcgaccg gctgtcggcc
     3661 gggccggact tcacgccggt gctgatgcgc cgtctgctgg cccagcgcgt gaaactgcag
     3721 ggcttcatcg tgtcggacca tggcgcacgg ctcgccgatt tccagcgcga catggccggc
     3781 tggctgcgcg aaggcaaggt gcgctaccgc gaagacgtgg tcgagggact ggagcatgcg
     3841 cccggcgcct tcatcggcct gctcaagggc agcaacttcg gcaagctggt gatccgcgtc
     3901 agccccggct gacggcggcc gctggatttt tgcggcaacg cggcacgcga actgatgcaa
     3961 actagacgtt cgttcaactg cgtcgacttg ccatgagcac tgtgcgcacc ggaagcgcca
     4021 aggccccgat cgaggtctac acgcacaagt tcttcacccc gccggcgtac gcgggcgtga
     4081 tccggcgcga ccagctgatc gaacgcatct tccagcacag cgcgcgcgtg gtgctgctgc
     4141 aaggccccgc cggccacggc aagtccaccg ccctgcagca gctgaaagcg gtgtgcgacg
     4201 agcgcggcca ggtcaccggc tggctcacgc tcgacgatgc cgacaacgac acccggcgct
     4261 tctacctgca tgtgcacgcg ctgctcaagt cgctgaagct gtccgaccgc aagggcgaag
     4321 gacatgacgg cggccgccgc cgctccgact ggctgctcga acgcatgcag cgcgccggca
     4381 agccggtggc gatcttcttc gacgatttcc agacgctgac caatcgcagc ctgctgatgt
     4441 tcttccgcga gctgttcgag cgcctgccgg acaacgtgcg tttctacatc ggcacgcgct
     4501 cgacaccgga actcggcctg ccgcggctgg tggtgaacca tcgcgcgctg atcctgcacg
     4561 ccgacgacct gcgcttctcg cccgccgagg tcgagcgctt cttcgccggc gggccacgcg
     4621 tgcagatcag cgccgaagaa attgccgcca tctaccgccg caccgagggc tggcccgccg
     4681 cgctgcagct gttccgcctg tcgctggcca gccccgacgt gcgccgctcg ctggacgatc
     4741 tcacgtcctc gcgtccgcgc gagctggccg agtacctggc cgacaacgtg ctggcgctgc
     4801 agccgcccga catgcgtgac ttcctgctgc gcaccgcgct gctgccgcgc ctgaccgctt
     4861 cgctgtgcca cgccgtcacc ggccgtgtcg atgcgcagga actgctgctg cggctggaac
     4921 gcagcgggct gttcgtgcgc agcctc
//
LOCUS       DASZZC010000705         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_311868,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000705 DASZZC010000000
VERSION     DASZZC010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311868"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1103)
                     /locus_tag="VHE37_14975"
     CDS             complement(261..1103)
                     /locus_tag="VHE37_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVT36892.1"
                     /translation="MRFTGTKDYVATDDLMMAVNAAVTLERPLLVKGEPGTGKTRLAQ
                     EVAGALDLPYFEWHVKSTTKAQQGLYEYDAVSRLRDSQLGESKVKDIANYIVKGKLWE
                     CFESDKRPLLLIDEIDKADIEFPNDLLRELDQMQFYVYETRQTISARHRPVIVITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMQRIVDVHFPSLKKNLLKEAMDVFFGLRELPGLK
                     KKPSTSELLDWLKLLMAEDIDPAALRETSVKKSLPPLYGALLKNEQDVHLFERIAFMA
                     RRSN"
     gene            1312..2364
                     /locus_tag="VHE37_14980"
     CDS             1312..2364
                     /locus_tag="VHE37_14980"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVT36893.1"
                     /translation="MRFRSTLLVGAAAVAVLVFAARIPPHAASASLMPLAPSASASEK
                     AALASGEVVVVLNSGDSSISLLDETTGAEIRRIPVGKEPHHLIITPDEKSLVVASSLT
                     NELLLLDPVTGAPTKRITHVTDPYQLGFSPDGKWLVTNGFALQRVDIYSHDHGSHLRL
                     ERRIHLPQTPSHMAFTADSQLLFVTLQDSDEIAAIDLPGQRLLWKMKTGKQPAGIRVT
                     GDGKYLLVGMTGDDYVAVIDWRTREEIKRIKTGRGAHNFLAAGDGRHVYVSNRVSDTI
                     SVLDEQTLTKSDEYHVPGGPDCMELSADRTRLWVTARWARKVVVVDLPTHTIARETEV
                     GRSPHGIYFRSHAAGT"
     gene            2361..3098
                     /locus_tag="VHE37_14985"
     CDS             2361..3098
                     /locus_tag="VHE37_14985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVT36894.1"
                     /translation="MKAFLAGALLALASFSAGAADGCAQPVHITIDTGSQSQAQVLAD
                     ILQRHHVRANFFLANERTVYGDFALDDGWADYWRARVAEGHVFGSHTLRHGRFAHDLP
                     GGAVQYIPQFGLDAGKPLTLTQADVCAELAAVDDRFVRLTGHRLAKLWRAPGGHTTPN
                     TLIAAQSCGWRHVGWSQAGFLGDELPSEQYPNSVLVGRAVRDIKPGDIVMLHMGIWSR
                     KQAFAPALDDVLTGLEARGLCFAPIER"
     gene            3100..4035
                     /locus_tag="VHE37_14990"
     CDS             3100..4035
                     /locus_tag="VHE37_14990"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVT36895.1"
                     /translation="MLGELQTWIFEHAVEPLLYATNQMALAEDAYDGIEAVLLGAVEI
                     ALLLILLRPLERWRPLEHWSDMRPVRTDVIYTLLHRLGGFALILYFSVRPLLLWLDGE
                     LHLRGIAPAPLESWVPGLADHPAASFLVYLVILDFADYWRHRLQHRLEWWWALHALHH
                     SQRQMSIWTDDRNHFLDDFIAAFWFAAVALAIGVPPSQFLLLAMTTRLVQSLAHINSR
                     IRFGWLGERLLVSPSYHRRHHAMGYGHEGRARGCNFATLFPIWDLLFGTADFSEHYDP
                     TGIRDQLDGRDYGEGLLRQQWLGFTRLARALLPSR"
     gene            complement(4032..4433)
                     /locus_tag="VHE37_14995"
     CDS             complement(4032..4433)
                     /locus_tag="VHE37_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVT36896.1"
                     /translation="MHKGSCLCGGVRFQIDAPLNEIGMCHCAMCRKASGTAFATNAPV
                     PEDKLRISGAELLSSYESSPGKQRVFCSRCGSPIYSRSAKRPGVVRIRVGTLDTPVGR
                     VPDYHFWVQDKADWYEIRDGLPQHRGVVPNR"
     gene            complement(4442..>5453)
                     /locus_tag="VHE37_15000"
     CDS             complement(4442..>5453)
                     /locus_tag="VHE37_15000"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HVT36897.1"
                     /translation="ISHLGRPKAGKLDPDASLTNVAAWLSHELGHSVPLISNWIDGVS
                     LQPGQIALGENTRFLKGEKEDDEALAKKMAALCDIYVMDAFGTAHRAECSTHGVAKFA
                     KVACAGPLLAAELEALGKALANPKRPLAVIVGGSKVSTKLDLLNSLADKADQLIIGGG
                     IANTFLAAAGHKVGKSLYEADMLDTARSILDKIRKRGGDIPLPEDVVVATEFSAEARA
                     QTRAVNEVEDDEMILDIGPKTRKKLAALIEQCGTIVWNGPVGVFEYDSFAGGTKAIAQ
                     AIASSKAFSLAGGGDTLSAIDKFHVADKLGYISTGGGAFLEFLEGKKLPAVAMLEQRA
                     QG"
BASE COUNT          866 a         1841 c         1753 g          993 t
ORIGIN      
        1 acaccgcagc tgacagcagc acgatattgc agccggtgcc gcgcccgatc gtcagcgccc
       61 cgctgcccag cggagtacgc gaatggacgt tgccggccgc gtcgcggacg atgacggtcc
      121 acatagcgct catacaaccc ctgtcacaaa ccggcacaaa aatagccgat taactttttt
      181 cagtacagcc cgcacccacg ggccctgcag catatccgcg gcgcggcgcc ctgcgacggc
      241 gttgcgcccc ggccccgcca tcaattcgaa cgccgcgcca tgaaggcgat gcgctcgaac
      301 agatggacat cctgttcgtt cttcagcagc gcgccgtaca gcggcggcag cgatttcttc
      361 acgctggtct cgcgcagcgc ggccgggtcg atgtcctcgg ccatcagcag cttgagccag
      421 tcgagcagtt cggaggtgga cggcttcttc ttcaggcccg gcagttcgcg caggccgaag
      481 aacacgtcca tcgcttcctt cagcaggttc ttcttcaggc tggggaagtg cacgtcgacg
      541 atgcgctgca tcgtctcctt gtccgggaag cggatgtagt ggaagaagca gcggcgcagg
      601 aaggcgtccg gcaactcctt ctcgttgttc gaggtgatga cgatcaccgg ccggtgccgc
      661 gcgctgatgg tctggcgcgt ctcgtagacg tagaactgca tctggtcgag ctcgcgcagc
      721 aggtcgttgg ggaactcgat gtcggccttg tcgatctcgt cgatcagcag cagcggacgc
      781 ttgtcgctct caaagcattc ccacagcttg cccttgacga tgtagtttgc gatgtccttg
      841 accttgctct cgcccagctg cgaatcgcgc aggcgcgaga cggcgtcgta ctcgtacagg
      901 ccctgctgcg ccttggtggt ggacttcacg tgccactcga aatacggcag gtcgagtgcg
      961 ccggccactt cctgcgccag gcgcgtcttg ccggtgcccg gctcgccctt gaccagcagc
     1021 gggcgctcca gcgtgacggc ggcgttgacc gccatcatca ggtcatcggt tgcaacgtaa
     1081 tccttggtgc cggtgaatcg cacgtcatgc tcctggaaac cgcaaaaatg acctgaccgc
     1141 acggtcggta ttgctgttat aaaatactat attaaagtaa cttaagttta tattttataa
     1201 cgacgttata atacttgcgg tgacggtggg gcgggcaatt taacccgtat ttttgtggaa
     1261 agcgatcccg ctattctgtg cgggacatca gtgacttcag ggatttttcc aatgcggttt
     1321 cgcagcactc tcttggttgg cgcggcagcg gttgcggtcc tggtgttcgc cgcgcgcatt
     1381 ccgccgcacg ccgccagcgc ctcgctgatg ccgctggcgc cttcggccag cgcgtcggag
     1441 aaggctgcac tcgcgtccgg cgaagtggtg gtggtgctca actccggcga cagctcgatc
     1501 agcctgctgg acgagaccac cggcgccgag atccgccgca tcccggtggg caaggagccg
     1561 caccacctga tcatcacgcc cgacgagaaa tcgctggtgg tggccagttc gctgaccaac
     1621 gagctgctgc tgctcgaccc ggtgaccggc gctccgacca agcgcatcac ccacgtcacc
     1681 gatccctacc agctgggctt ttcgcccgat ggcaaatggc tggtgaccaa tggcttcgcg
     1741 ctgcagcgcg tggacatcta ctcgcacgac cacggctcgc acctgcggct ggagcgacgc
     1801 atccacctgc cgcagacacc cagccacatg gccttcaccg ccgacagcca gctgctgttc
     1861 gtcaccctgc aggacagcga cgagatcgcc gccatcgacc tgcccggcca gcgcctgctg
     1921 tggaagatga agaccggcaa gcagcccgcc ggcatccgcg tcaccggcga cggcaagtat
     1981 ctgctggtcg gcatgaccgg cgacgactac gtggcggtga tcgactggcg cacgcgcgag
     2041 gagatcaagc gcatcaagac cggccgcggc gcgcacaact tcctggccgc cggcgacggc
     2101 cgccacgtgt acgtgtcaaa ccgcgtgtcc gacaccatca gcgtgctcga cgagcagacg
     2161 ctgaccaaaa gcgacgagta ccacgtgccc ggcgggccgg actgcatgga actgtcggcc
     2221 gaccgcacgc gcctgtgggt gaccgcgcgc tgggcgcgca aggtggtggt ggtggacctg
     2281 cccacgcaca ccattgcgcg cgaaaccgag gtcgggcgtt cgccgcacgg catctacttc
     2341 cgctcgcacg cagccggcac atgaaagcgt ttctcgccgg cgcgctgctt gcgctggcgt
     2401 ccttctccgc tggcgcggct gacggttgcg cgcagccggt gcatatcacc atcgacaccg
     2461 gcagccagtc gcaggcgcag gtgttggcgg acatcctgca gcgccaccat gtccgcgcca
     2521 attttttcct ggccaacgag cgcacggtct acggcgactt cgcgctcgac gacggctggg
     2581 cggactactg gcgcgcgcgc gtcgccgaag gccacgtgtt cggcagccac acgctgcgtc
     2641 acggccgctt cgcgcatgac ctgccgggcg gtgcggtgca gtacatcccg cagttcggcc
     2701 tcgatgccgg caagccgctc acgctgacgc aggccgacgt ctgcgcggag ctcgccgcgg
     2761 tcgacgaccg cttcgtgcgc ctcaccggtc accgcctggc caagctctgg cgcgcgccgg
     2821 gcggccacac cacacccaac acgctgatcg cggcgcagtc ctgcggctgg cggcacgtcg
     2881 gctggtcgca ggcgggattt ctcggcgacg agctgccgtc ggagcagtat cccaacagcg
     2941 tgctggtggg ccgcgcggtg cgcgacatca agcccggcga catcgtcatg ctccacatgg
     3001 gcatctggtc gcgcaagcag gcgttcgcgc cggcgctgga cgacgtgctg acgggacttg
     3061 aagcgcgcgg gctgtgcttc gcaccgatcg aacgctgaca tgctcggcga actgcagacc
     3121 tggatcttcg agcacgcggt cgagccgctg ctctacgcga caaaccagat ggcgctggcg
     3181 gaagacgcct atgacggcat tgaggcggtg ctgctgggcg ccgtcgagat cgcgctgctg
     3241 cttatcctgc tgcgcccgct ggagcgctgg cgtccgctgg aacactggag cgacatgcgc
     3301 cccgtgcgca ccgacgtgat ctacacgctg ctgcaccggc tcggcggctt tgcgctcatt
     3361 ctctacttca gcgtgcgccc gctgctgctg tggctggacg gcgagctgca cctgcgcggc
     3421 atcgcgccgg cgccgctgga atcctgggtg ccgggcctgg ccgatcaccc cgccgccagc
     3481 ttcctggtct acctggtgat cctcgatttc gccgactact ggcgccaccg gctgcagcat
     3541 cggctcgaat ggtggtgggc gctgcacgcg ctgcatcaca gccagcggca gatgagcatc
     3601 tggaccgacg accgcaatca tttcctcgac gatttcattg cggcgttctg gtttgccgcg
     3661 gtggcgctgg ccatcggcgt gccgccgtcg cagttcctgc tgctggcgat gaccacgcgg
     3721 ctggtgcaga gcctggcgca catcaattcg cgcatccggt tcggctggct cggcgaacgc
     3781 ctgctggtca gcccgagcta tcaccgtcgg catcatgcga tgggttacgg ccacgaaggc
     3841 cgcgcgcgcg gctgcaattt tgccacgctg tttccgatct gggatctgct gttcggcacc
     3901 gccgatttca gcgagcacta cgacccgacc ggcattcgcg accagctcga cggacgcgac
     3961 tacggcgaag gcctgctgcg ccagcagtgg ctgggtttca cgcggctggc gcgcgcgctg
     4021 ctgccctcgc gctagcggtt ggggacgacg ccgcgatgct gcggcagccc gtcgcggatc
     4081 tcgtaccagt cggccttgtc ctgcacccag aagtgatagt ccggcacgcg ccccactggc
     4141 gtgtcgagtg tgccgacgcg gatgcggacc acgcccggcc tcttcgccga gcggctgtag
     4201 atgggcgaac cgcagcgcga gcagaagacg cgctgcttgc cggggctgga ttcgtatgag
     4261 ctcagcagtt ctgcgccgct gatgcgcagt ttgtcttccg gcaccggcgc attggtggcg
     4321 aacgccgtgc ccgaggcctt gcggcacatg gcgcaatggc acatgccgat ttcgttgagc
     4381 ggtgcgtcaa tctggaaacg cacgccgccg cacaggcaac tgcctttgtg catattgatg
     4441 ctcagccttg cgcgcgctgc tccagcatcg ccaccgccgg cagcttcttg ccttcgagga
     4501 attccaggaa cgcgccgccg ccggtggaga tgtagccgag cttgtcggcc acatggaact
     4561 tgtcgatcgc cgagagcgtg tcgccgccgc cggcgagcga gaacgccttg ctcgacgcga
     4621 tggcctgggc gatggccttg gtgccgccgg cgaagctgtc gtactcgaac acaccaaccg
     4681 ggccgttcca cacgatggtg ccgcattgct cgatcagcgc ggccagcttc ttgcgcgtct
     4741 tcgggccgat gtcgaggatc atctcgtcgt cctcgacttc gttcactgcg cgcgtctgtg
     4801 cgcgcgcctc ggccgagaat tccgtcgcca ccaccacgtc ttccggcagc ggaatgtcgc
     4861 cgccgcgctt gcggatcttg tcgagaatgc tgcgcgcggt gtcgagcatg tcggcctcgt
     4921 acagcgactt gccgaccttg tggccggccg ctgccaggaa agtgttggcg atgccgccgc
     4981 cgatgatcag ctggtcggcc ttgtcggcca gcgagttgag caggtcgagc ttggtcgaca
     5041 ccttgctgcc gccgacgatc accgccagcg ggcgtttggg attggccagc gccttgccca
     5101 gcgcttcgag ctccgccgcc agcagcgggc cggcgcaggc gaccttggca aacttcgcca
     5161 cgccgtgcgt cgagcactcg gcgcggtgcg cggtgccgaa agcgtccatc acgtagatat
     5221 cgcacagcgc ggccatcttc ttcgccagcg cctcatcatc ttccttttcg cccttgagga
     5281 agcgcgtgtt ctcacccaat gcgatctggc ccggctgcag gctcacaccg tcgatccagt
     5341 tgctgatcag cggcacgcta tgacccagct cgtgcgacag ccaggcggcg acgttggtca
     5401 gcgaggcgtc ggggtcgagc ttgccggcct tgggccggcc caggtgcgag atc
//
LOCUS       DASZZC010000706         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1211561,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000706 DASZZC010000000
VERSION     DASZZC010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211561"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="VHE37_15005"
     CDS             complement(<1..1688)
                     /locus_tag="VHE37_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36898.1"
                     /translation="MALQAGLLRGGLVMVATLALCSCNFPFSDDSGSGSSTTPGSGVD
                     ISGTALLGPLNGATLRLFPVTPTGGNGAPSVTAEAGPTPGSFKFHLDQVPSGPFRVCA
                     EGGTYTDEASGNTGVQNKLSELCATVPSNSRTVNVNVLTALANSLLKGLMSRPSPPPL
                     VDALITARNTIQTFANLTAPPDEVTPVFHPSDSTDPNGDDLKAGLIIGGYSQLDNRDA
                     ATCPPPSDAPQQQTGALIADIADGVFDGKDGGTPIQVTCNNTPQNMPVGRGTAELSTA
                     LTEFVNQQPAEDQQADNQTASDIKVSVTNGDTAPPQVKVTPSQGLISIDQVNNIAYVP
                     IYTFDANGNARIAVVDLKNPPADPANIPTVALVDANDPDANHQAKQPVATSFDPVGRL
                     VYVEARRADNGVNVYVIDPSNNSVVNVVQTGLTHNGTFGGIIADPQNHQVVVTGTSSL
                     SLLDISATPAVLKTDPNNSGNSVFSINGTDSIALNFDTQKLFVSADGSNQIIDLSIFP
                     PVQHGFSFAPNTADGVGFDNLTGLVVLSAEVTSDEVYVYNFVELASDQSTAPMTR"
     gene            complement(1837..>2023)
                     /locus_tag="VHE37_15010"
     CDS             complement(1837..>2023)
                     /locus_tag="VHE37_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36899.1"
                     /translation="VALAATDLPKCTRVHPRSQICSLTGVIGPGSSTLTGTSCVTMPY
                     QDRGSWVHRVGYEGAAQ"
BASE COUNT          354 a          586 c          673 g          410 t
ORIGIN      
        1 cgtgtcatgg gtgcagtcga ctggtcgctc gccagttcga caaagttgta gacatacact
       61 tcatcggagg tcacttctgc gctgagcacg accagtccgg tgagattgtc gaaaccgacg
      121 ccatcggcag tgttcggcgc aaaactgaag ccatgctgca ccggggggaa tatcgagaga
      181 tcaatgatct ggttggaccc gtctgccgac acgaacagct tctgcgtatc gaagttgaga
      241 gcaatggaat cggtgccgtt aattgagaac accgaattgc ccgagttgtt cggatcagtc
      301 ttcagcacgg ccggagtagc cgagatatcc agcaggctca ggctcgatgt accggtgacc
      361 actacctggt gattttgcgg atcggcgatg atgccgccga aggtgccgtt atgcgtgagg
      421 ccagtctgga cgacattgac aacgctgttg ttggacgggt cgatgacgta gacattgacc
      481 ccgttatcgg cacgacgcgc ctcgacgtac accaggcggc cgaccgggtc gaagctggtg
      541 gccaccggct gcttggcctg gtgattggcg tccgggtcat tcgcgtcgac cagcgcaacc
      601 gtcggaatat ttgcgggatc ggccggcgga ttcttcaggt ccaccacggc gatgcgcgcg
      661 ttgccgttgg catcgaacgt atagatcggc acgtaggcga tgttgttgac ctgatcgatg
      721 gaaatcagcc cctggctcgg cgtcaccttg acttgcggtg gcgcggtgtc gccattggtc
      781 accgagacct tgatgtcgct ggcggtctgg ttgtccgcct gctggtcttc cgccggctgc
      841 tggttgacga actccgtgag cgccgtgctc agttcggcgg tgccgcgacc gaccggcatg
      901 ttctgcggcg tgttgttgca ggtgacctgg atcggcgtgc cgccgtcctt gccgtcgaac
      961 acaccgtcgg cgatatcggc aatgagcgcg ccggtctgct gctgcggtgc atcgctgggc
     1021 ggcgggcagg tggcggcatc gcggttatcc agctgcgaat aaccgccgat gatcagcccg
     1081 gccttgagat cgtcgccgtt cggatcggtg gaatccgacg ggtggaacac gggcgtgacc
     1141 tcgtccggcg gcgcggtcag gttggcgaag gtctggatgg tgttgcgcgc cgtgatcagc
     1201 gcgtcgacca gcggcggcgg tgacggccgc gacataagtc ccttgagcag gctgttggcc
     1261 aacgcggtga gtacattgac gtttacggtg cgcgaattgg aagggaccgt ggcgcagagt
     1321 tccgacagct tgttttgcac gccggtgttg ccgctggctt catcggtgta ggttccgcct
     1381 tcggcgcata cgcggaatgg cccgctcggc acctggtcga ggtggaactt gaacgatcct
     1441 ggggttgggc cggcttcggc ggtcaccgac ggcgcgccgt tgccgccagt gggcgtaact
     1501 ggaaacaacc tgagcgttgc accgttcagc gggcccagca gcgcagtgcc actgatgtcg
     1561 acacctgaac ctggcgtcgt ggaggaaccc gacccgctat cgtcggagaa tgggaaattg
     1621 cacgagcaca gcgcgagcgt agctaccatg accaatccgc cgcgcaacaa cccagcttgc
     1681 agagccatga cgtttcctcc ctttgcacgc ggcggaacgc atcgcccgga tgctcatcga
     1741 tgcggcgccg gctgcgggtg gactgtacgc ccaaattcct tcgatgcaac ccctttaacg
     1801 tggcgtgcgt gaaccgctta acggtttcac gccgttttat tgcgcggcac cttcatagcc
     1861 cacgcgatgc acccaggagc cgcggtcctg ataaggcatg gtgacgcagc tggtgcccgt
     1921 caatgtgctg gaaccggggc cgatcacgcc ggtgagcgaa cagatctgac tgcgtggatg
     1981 cacacgggtg catttcggca ggtcggtcgc tgccaatgcc acg
//
LOCUS       DASZZC010000707         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_824695,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000707 DASZZC010000000
VERSION     DASZZC010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824695"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..>2046
                     /gene="purL"
                     /locus_tag="VHE37_15015"
                     /gene_synonym="purI"
     CDS             136..>2046
                     /gene="purL"
                     /locus_tag="VHE37_15015"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HVT36900.1"
                     /translation="MSITVLPGAEALSAFRITRLAQRLAAHGVVPTKIRTLDFYLVDA
                     SGSAEAELRRLLGPGPKDFPRAALTLFVVPRVGTLSPWSSKATDIARVCGLAGVQRIE
                     RGLAYLLDGASELPPAALADLHDPMTESVLTRQEQLQHVFDAQPRRPLRSVDLLKLGI
                     GALEVANRDWGLALSREEMEYLTAHYTRAGRNPTDAELMMFAQVNSEHCRHKIFNAQF
                     VVDGQEQDRSLFQMIKLTHAAAPQGVLSAYKDNAAVIEGAAGMRLFPDTDHVWRGRME
                     DIAILMKVETHNHPTGISPHPGAATGAGGEIRDEAATGRGAKPKAGLCGFTVANLRIP
                     DAVQPWEAELPRPPRMASALSIMLEGPIGAAAYNNEFGRPNLAGYFRTYEAVTPDGKN
                     RGYHKPIMIAGGYGNIRVPHVEKHEVSEGALLVVLGGPAMLIGLGGGAASSMATGASS
                     AELDFASVQRANPELQRRCQEVVDRCWSLGADNPILSIHDVGAGGLSNALPELVHADD
                     RGGLFRLRDILSTDPALSPMEIWCNESQERYVLAIAPARLPVLEQACVRERCPYAVVG
                     RATAERQLVVEDALDHTHVVDMPMPVLLGKPPRMRRETRREPSKFPPLKTAGIALNEA
                     AERVLRLPCVASK"
BASE COUNT          361 a          695 c          684 g          306 t
ORIGIN      
        1 ttgcgtgtta ttaattatta tataaaatat aaaacaatca ataattaata acaatgaaaa
       61 aggccaggcg gtgaggcaaa aaatgcgaaa atgcgcgcgc cttttccgct acgcgcatac
      121 gcctcgaaag gtctcatgtc catcaccgta cttcccggag ccgaagccct ctcggcattc
      181 cgcatcaccc gtcttgccca gcggctggct gcgcacggag tggttccgac aaaaatccgc
      241 acgctggact tctacctggt ggatgccagc ggcagtgcgg aagccgaact gcgccggctg
      301 ctcggcccgg ggccgaaaga tttcccgcgc gcggcgctga cgctgttcgt ggtgccgcgc
      361 gtcggcacgc tgtcgccatg gtcgagcaag gccaccgaca tcgcgcgcgt gtgcgggctg
      421 gccggcgtgc agcgcatcga gcgtgggctg gcctacctgc tcgacggcgc aagcgaactg
      481 ccgccggcgg cactggccga cctgcacgac ccgatgaccg agtcggtgct gacgcgacag
      541 gagcagctgc agcacgtgtt cgatgcgcag ccgcgccggc cgctgcgcag cgttgacctg
      601 ctcaagctgg gcatcggcgc gctggaagtg gccaaccgcg actggggcct ggcgctgtcg
      661 cgcgaggaga tggagtacct caccgcgcat tacacgcgcg ccgggcgcaa cccgaccgat
      721 gccgagctga tgatgttcgc gcaggtcaac agcgagcatt gccgccacaa gatcttcaac
      781 gcgcagttcg tggtcgacgg ccaggagcag gaccgctcgc tgttccagat gatcaagctg
      841 acgcacgcgg ctgcgccgca gggcgtgctg tcggcgtaca aggacaacgc cgcggtgatc
      901 gagggcgccg ccggcatgcg cctgtttccc gataccgacc acgtgtggcg cgggcgcatg
      961 gaagacatcg ccatcctgat gaaggtggag actcacaacc acccgaccgg aatttcgccg
     1021 cacccgggcg cggcgaccgg cgccggcggc gagatccgcg acgaggccgc caccggccgc
     1081 ggcgccaagc ccaaggccgg gttgtgcggt ttcaccgtgg ccaacctgcg cattcccgat
     1141 gcggtgcagc cgtgggaagc ggaattgcca aggcctccgc gcatggccag cgcgctctcg
     1201 atcatgctcg aaggcccgat cggcgccgct gcctacaaca acgaattcgg ccggcccaat
     1261 ctcgccggct atttccgcac ctacgaagcg gtcacgcccg acggaaaaaa ccgcggctac
     1321 cacaagccga tcatgatcgc cggcggctac ggcaacatcc gcgtgccgca cgtggagaag
     1381 cacgaagtca gcgagggcgc gctgctggtg gtgctcggcg gcccggccat gctcatcggc
     1441 ctgggcggcg gcgcggccag ctccatggcc accggcgcct cgagcgcgga actggacttc
     1501 gccagcgtgc agcgcgccaa tcccgagctg cagcggcgct gccaggaagt ggtggatcgc
     1561 tgctggtcgc tgggcgcgga caatcccatc ctgtcgatcc acgacgtcgg cgcgggcggc
     1621 ctgtccaatg cgctgcccga gctggtgcac gcggacgatc gcggcgggtt gttccgcctg
     1681 cgcgacatcc tcagcaccga tcccgcgctg tcgccgatgg aaatctggtg caacgaatcg
     1741 caggagcgct acgtgctggc catcgcgccg gcgcgcctgc cggtgctcga gcaggcctgc
     1801 gtgcgcgagc gctgccctta cgccgtggtc ggccgcgcca ccgccgagcg ccagctggtg
     1861 gtggaagacg cgctggacca cacgcacgtg gtcgacatgc ccatgccggt gctgctgggc
     1921 aagccgccgc gcatgcgccg cgagacgcga cgcgaaccgt cgaagtttcc gccgctgaag
     1981 accgcaggca ttgcgctgaa cgaagccgcc gagcgcgtgc tgcgcctgcc ctgcgtggcg
     2041 tcgaaa
//
LOCUS       DASZZC010000708         5754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_825056,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000708 DASZZC010000000
VERSION     DASZZC010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825056"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VHE37_15020"
     CDS             complement(<1..1256)
                     /locus_tag="VHE37_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HVT36901.1"
                     /translation="MIRLLALSLLGAMLLLAGCARSSTPAMAAATAAPAQQDALTLVS
                     DTALSPRLHELIVHSPALGTDTGVRLLLPAGYDENSPTRYPVLYLLHGCCDVGDQGYQ
                     AWTTNTDVEAFTATMPLLIVMPMSGKGGFYADWYNNGAGGTPRWETHHIRELLPWIEA
                     HYRVRSDRAGRMLAGLSMGGHGSFTYGAKYPDLFASAAAYSPVIDTNTPRGIALTEAL
                     AGLDLGIPGSLFGLRPTEEVRWRGHNPWDLAENLAPLKLYIRTGNGNQDGVPVDPIES
                     IVNEMAHNVHDQLDVLGIAHSFVDYGDGTHSWTDWQADLHATLPEQLAYAQSNPPPPE
                     TVTYRSIDPQFSVFGWSVSLQRNVLEFARLGAATAAGFTLSGSGAGAVMTPAFYAPGE
                     NCVVRIDGKQSTLAADASGRLHLAVDL"
     gene            complement(1271..2293)
                     /locus_tag="VHE37_15025"
     CDS             complement(1271..2293)
                     /locus_tag="VHE37_15025"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36902.1"
                     /translation="MTCRWLGLLAGIAALSGCSNGSDGNPPAQTPAGADTSGLPGWAI
                     GPFTRYAGNPVMSPEGTGWESLQALNPGVVEVGGHYQMIYRGIEPAMLSQIGMATSDD
                     GIQWTRYANNPVIPNDVAGGVLGYEDPRLVYLDGTYYAFFTRFTTGSTDLFEATSTDL
                     VHWNMTGVVAPGTKNGAVVTDPHGRPVQINGLYTMYVGEMEKFSYWTSPDMLHWTQGG
                     TVDLGYGDLGLPDEVCVAVTDYPRRDGSTGHDIVLFTAGHLVPTGTWYYAISETLYSR
                     EQPDHQIDRLPGPVLLPTEPYELAGVTPVTVFMNSILLNDQGLWMMHYGAADTRIGLA
                     TAPSRN"
     gene            2516..4021
                     /locus_tag="VHE37_15030"
     CDS             2516..4021
                     /locus_tag="VHE37_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36903.1"
                     /translation="MACALASACGTRSGAAASGATAAAQAWEQPVPKAQCGPADVPEP
                     ALQGQVTLLERASGFQGTHCNLELVGQWAGEGASWMQASLGDCAYYDQAVGMFGDSEV
                     IPRAHHQHPGTVVIDATDSSHPVASEYLTSASMIDPWESLKVNEKRKLLAGVFGAQGQ
                     GGPQFDVYDIAADCRHPQLLASVTLSDSTLAGHEGNFSPDGMTYYGADNSHNIWYAID
                     LADPARPRLIAEFKPQVSNVHGLSLSTDGNRGYFVQASVPSPVYQPGAAPANGFYIFD
                     TSDVQQRRPNPELRLISQAQWSDGAQAQHTLPFFIHGHAYLLALDEWGAAGSGSAATW
                     AQACAQGMPPYGFARIFDIGDETRPSLVSKLMLQTHDPANCWDVIGDTSENLNFGYDS
                     HYCTVDDADDAQIAACSYFESGLRVFDIRDPHHPKEIAYYNPPARPGYQAGSMYDITG
                     VCGSADWTSAQPRIRRDRGEIWFTSQCNGFQIVKFSNGVLPAATAKSKTSS"
     gene            complement(4036..4668)
                     /locus_tag="VHE37_15035"
     CDS             complement(4036..4668)
                     /locus_tag="VHE37_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT36904.1"
                     /translation="MLMAASGTPRRARLSRAQQRAARTRDILDAAWAIYREQGYEAAT
                     IEAVAEHAGISRMPVYSLFGDKQNLYYELSNMLLGQLVQVMVEPLHSAPSLRAGLERM
                     ARLAATPQPRTSGHGPESLFHVVQVIGLSRPDIAARQKKGAKDVVDAFAAAIRKAPLQ
                     RGERLRASPEIVASLIVSLINGLSNVETQTGRKFIRASDLAAMFCAAAFR"
     gene            4800..>5754
                     /locus_tag="VHE37_15040"
     CDS             4800..>5754
                     /locus_tag="VHE37_15040"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT36905.1"
                     /translation="MSNGRKAGMHRRGRCAIAALLLCAAGSAQADDAMMAPADAAPPP
                     VATIPVEAAPAQEPLKSRKDKDDKSGAARLDDVVVTADYRPQGVQDVAGSAQAFDGQQ
                     LDQSGAAGMQDYLLQVPSVSLQPSGNGKLNIAMRGVSNINASDLGFNDGSPTVGVYLN
                     DVAVQGSGVFPDLRIFDLDRIEVLKGPQGTLYGEGSMGGSIKMVTAPVDYSRWVVRGE
                     AMYDLTAGGAPGNEVRGALNIPLVNDTLGLRLVGTDRLDGGFVDYTQLGLKNANSDRT
                     ESARAILSWKPTQTLGLDYTFLYNRDQRNQFPVVDEGKQQDM"
BASE COUNT          969 a         1869 c         1952 g          964 t
ORIGIN      
        1 agatcgacag cgaggtgcag gcgcccgctg gcgtcggctg caagcgttga ctgttttcca
       61 tcgatgcgca ccacgcagtt ttcgcccggc gcgtagaacg ccggtgtcat caccgcgccg
      121 gcgccgctgc ccgagagcgt gaatcctgcg gcggtcgctg cgcccagccg ggcgaattcg
      181 agcacgttgc gttgcaggct cacgctccag ccgaacaccg agaactgcgg atcgatgctg
      241 cggtaggtga cggtttcggg cggcggcgga ttggactgcg catatgccag ctgctcgggc
      301 agcgtggcgt gcaggtccgc ctgccagtcg gtccagctgt gcgtgccgtc gccgtagtcg
      361 acgaagctgt gcgcgatgcc cagcacgtcg agctggtcgt gcacgttatg cgccatttcg
      421 ttaacgatgc tttcgatcgg atcgaccggc acgccgtcct ggttgccgtt gccggtgcgg
      481 atgtagagct tgagcggtgc gagattttct gcgaggtccc acggattgtg tccgcgccag
      541 cgtacctctt cggtcggacg caggccgaac agcgagccgg gtatgcccaa gtcgagcccc
      601 gccagcgctt cggtcagcgc aatgccgcgc ggggtgttgg tgtcgatcac cggcgagtac
      661 gccgcggcgc tggcgaacag gtcgggatat ttcgcgccgt aggtgaagga gccgtggccg
      721 cccatcgaca ggcctgccag catgcggccg gcgcggtcgc tgcgcacgcg gtaatgcgcc
      781 tcgatccatg gcagcagttc gcggatatgg tgcgtctccc agcgcggcgt accgccggcg
      841 ccattgttgt accagtcggc gtagaagccg cccttgccgc tcatcggcat gacgatcagc
      901 agcggcatgg ttgcggtgaa tgcttccacg tcggtgttgg tggtccacgc ctggtaaccc
      961 tggtcgccca cgtcgcagca gccgtgcagc aggtagagca ccgggtatcg cgtgggagaa
     1021 ttttcgtcgt agccggcagg cagcagcagg cgcacaccgg tgtccgtgcc gagcgcagga
     1081 ctgtgcacga tcagctcgtg caggcgcggc gacagcgcgg tgtcgctgac cagcgtcagc
     1141 gcatcctgct gcgccggcgc tgcggtggct gcggccatcg ccggcgtgct tgaccgagcg
     1201 cagccggcca gcagcagcat cgcgccgagc agcgacagcg cgagcaaccg gatcatcacg
     1261 cgaccctcac ctagttgcgc gaaggcgcgg tggccaggcc gatgcgcgtg tccgcggcgc
     1321 cgtaatgcat catccacagg ccctggtcgt tgagcaggat cgagttcatg aataccgtca
     1381 ccggtgtcac gccggccagt tcgtagggct cggtcggcag cagcaccggg cctggcaggc
     1441 ggtcgatctg gtggtcgggt tgttcgcgcg aatacagcgt ctcgctgatc gcgtagtacc
     1501 aggttcccgt gggcaccagg tgcccggcgg tgaacagcac gatgtcgtgg ccggtgctgc
     1561 cgtcgcgccg cgggtagtcc gtcactgcca cgcacacttc gtcgggcagg ccgagatcgc
     1621 cgtagccgag atcgaccgtg ccgccctgcg tccagtgcag catgtcgggc gaggtccagt
     1681 acgagaattt ttccatctcg ccgacataca tcgtgtacag gccgttgatc tgcaccgggc
     1741 gcccgtgcgg atcggtcacc accgcgccgt tcttggtgcc cggcgcgacc acgccggtca
     1801 tgttccagtg gaccaggtcg gtcgaggtgg cctcgaacag gtcggtgctg ccggtggtga
     1861 acctggtgaa gaacgcgtag taggtgccat cgaggtagac caggcgcggg tcttcgtagc
     1921 cgagcacgcc gccggcgaca tcgttgggga tcaccgggtt gttcgcgtag cgcgtccact
     1981 ggatgccgtc atcgctggtg gccatgccga tctgcgacag catcgccggc tcgatgccgc
     2041 gatagatcat ctggtagtgt ccgccgactt ccaccacgcc ggggttgagc gcctgcagcg
     2101 actcccagcc ggttccctcc ggcgacatga ccgggttgcc ggcgtagcgc gtgaacggtc
     2161 cgatggccca gccgggcagg ccgctggtat ccgcgccggc gggtgtctgc gcgggcgggt
     2221 tgccatcact gccattgctg cagccactga gggcggcgat gcctgccagc aggccaagcc
     2281 atctgcatgt catgcgcatc tctcctcgac gcattcgcgg ttcttatacg ttgacaatat
     2341 ttatacatcg tcaacattta tacattgtgt attttaaatt tataacctag agtcgccggc
     2401 caggcaacca ggaacaggcg caggggagag cgaggccatg ttcaccacgg acctcagcac
     2461 atgcaggccg cgcacagcga tggagaatcg cgccgtcgcc tggctcggaa tcgcattggc
     2521 ctgcgcactc gcatccgcct gcggtacccg atcgggcgcg gcggcatcgg gcgccaccgc
     2581 cgcggcgcag gcctgggagc agccggtgcc caaggcgcag tgcgggccgg ccgatgtgcc
     2641 cgagccggcg ctgcaggggc aggtcacgct gcttgagcgc gccagtggtt tccagggcac
     2701 gcactgcaac ctggaactgg tggggcaatg ggcgggcgaa ggtgccagct ggatgcaggc
     2761 gtcgctcggc gactgtgcct actacgacca ggccgtcggc atgttcggcg actccgaggt
     2821 gatcccgcgc gcccatcacc agcaccccgg cacggtggtc atcgacgcga cggactcaag
     2881 tcatccggtg gcgtccgaat acctgaccag cgcatcgatg atcgatccgt gggagtcgct
     2941 caaggtcaac gaaaaacgaa agctgctggc cggcgtattc ggcgcgcagg ggcagggcgg
     3001 accgcagttc gacgtctacg acatcgccgc ggattgccgg catccgcagc tgctggccag
     3061 cgtgaccctg tccgacagca cgctggcggg acacgaggga aatttttccc cggacggcat
     3121 gacctactac ggcgccgaca acagccacaa catctggtac gcgatcgacc tggccgatcc
     3181 cgccaggccg cgcctgatcg cggaattcaa gccgcaggtc agcaatgttc atggcctgtc
     3241 gctcagtacc gatggcaatc gcggttactt cgtgcaggcc agcgtgccgt cgccggtgta
     3301 ccagcccggc gccgcgccgg cgaacggttt ctacatcttt gataccagcg acgtgcagca
     3361 gcggcggccc aacccggaac tgcggctgat ttcgcaggcg cagtggagtg acggcgcaca
     3421 ggcgcagcac acgctgccgt ttttcatcca cggacatgcg tacctgctgg cgctggacga
     3481 atggggcgct gccggttcgg gcagcgcggc aacctgggcg caggcctgcg cgcagggcat
     3541 gccgccgtat ggctttgcgc gcatcttcga catcggtgac gagacccgcc cgtcgctggt
     3601 gtccaagttg atgctgcaga cacacgatcc ggcgaactgc tgggacgtga tcggcgatac
     3661 cagcgagaac ctcaacttcg gctacgacag ccactactgc acggtggacg acgcggacga
     3721 cgcgcagatc gccgcgtgca gctatttcga gtccggcctg cgcgtgttcg acatccgcga
     3781 tccgcatcac cccaaggaaa tcgcctacta caacccgccg gcgcggccgg gctaccaggc
     3841 cggctcgatg tatgacatca ccggtgtctg cggcagcgcc gactggacta gcgcgcagcc
     3901 gcgcatccgg cgcgaccgcg gcgagatctg gttcaccagc cagtgcaacg gcttccagat
     3961 cgtgaaattc agcaacggcg tgctgcctgc agctacagcg aagtcgaaga cctcgtcctg
     4021 accgcgacga tgacgtcagc gaaacgccgc ggcgcagaac atcgccgcga ggtcggaggc
     4081 gcggatgaac ttgcggccgg tctgggtctc gacgttggac aggccgttga tcagcgagac
     4141 gatcagcgaa gcgacgattt ccgggctagc gcgcagccgc tcgccgcgct gcagcggcgc
     4201 cttgcggatc gccgccgcga acgcgtcgac cacatccttg gcgcccttct tctgccgcgc
     4261 ggcgatgtcg ggacggctga ggccgatcac ctgcaccacg tggaacaggc tctccgggcc
     4321 atggccggag gtgcgcggct gcggcgtggc ggccagccgg gccatccgct cgaggccggc
     4381 gcgcagcgac ggcgcgctgt gcagcggctc gaccattacc tgcaccagct ggccgagcag
     4441 catgttggac aattcgtagt aaagattctg tttgtcgccg aacagtgagt agaccggcat
     4501 gcgcgagatg ccggcgtgct cggccaccgc ctcgatggtc gccgcttcgt aaccctgttc
     4561 ccggtagatg gcccaggcgg catcgaggat gtcgcgcgtg cgcgcggcgc gctgctgcgc
     4621 gcggctgagc cgtgcgcgcc gcggggttcc acttgctgcc atcagcatcg ggcactccgg
     4681 ccgtaggttt gcgttacgcc ggaaatatag gttataaact ttcaatacac aatgtataaa
     4741 cagcgacgat gtatttaaat cgctgatgta tagattatgc ggcgcgagga gagggcttga
     4801 tgagcaacgg gcggaaagcc ggcatgcacc ggcgcgggcg ctgcgcgata gcggcactgc
     4861 tgctgtgcgc cgcgggttct gcgcaggctg acgacgccat gatggcgccg gcggatgccg
     4921 cgccgccgcc ggtggccacc atcccggtcg aggccgcgcc cgcgcaggaa ccgctgaaat
     4981 cccgcaagga caaggacgac aagtccggcg ccgcgcgcct ggacgacgtg gtggtcacgg
     5041 ccgactaccg cccgcagggc gtgcaggacg tggccggcag cgcgcaggcc ttcgacggcc
     5101 agcagctcga ccagtccggc gccgccggca tgcaggacta cctgctgcag gttcccagcg
     5161 tatcgctgca gccgtctggc aacggcaagc tcaacatcgc catgcgcggc gtgtcgaaca
     5221 tcaatgccag cgacctgggc ttcaacgacg gctcgcccac cgtcggcgtt tatctgaacg
     5281 acgtcgcggt gcagggctcg ggcgtgtttc ccgacctgcg catcttcgat ctcgaccgca
     5341 tcgaggtgct gaagggaccg cagggcacgc tttacggcga aggctcgatg ggcggctcga
     5401 tcaagatggt gaccgcgccg gtcgactaca gccgctgggt agtgcgcggc gaggccatgt
     5461 atgaccttac ggctggcggc gcgccgggca acgaggtgcg cggcgcgctc aacatcccgc
     5521 tggtgaacga cacgctggga ctgcgtttgg tcggcaccga ccggctcgac ggcggcttcg
     5581 tcgactacac ccagcttggc ctcaagaacg ccaactccga ccgcacggag tcggcgcgcg
     5641 ccatcctgag ctggaagccg acgcagactc tcggtctcga ttacaccttc ctgtacaacc
     5701 gcgaccagcg caaccagttc ccggtggtgg acgaaggcaa gcagcaggac atga
//
LOCUS       DASZZC010000709         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1081947,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000709 DASZZC010000000
VERSION     DASZZC010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081947"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2384)
                     /locus_tag="VHE37_15045"
     CDS             complement(<1..2384)
                     /locus_tag="VHE37_15045"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HVT36906.1"
                     /translation="MDETGGMVMMQGFRRLWAPALILWSGAALAGPVLKLQPTFIAFH
                     NVNVGADSTVHAVTVSNTGDAALTFKANHITGANAGDFPMTTTCGATLAAGASCTVSI
                     TFHPRAGGDRAASLTVTSNAPGNPHTTTLTGVGLQTDAAAQPSTLDYGTLLIDTSATQ
                     TTSIVNVGNLPLHVGSVSLTGTHADQFALVHNGCSNPVGPTHSCNLSVRFTATATGKV
                     EARLLVASDAPHSPTAIVLAGAGRLPEPGNISMVAPSKDAAQADGSVEIAVARTDGWD
                     GAVSVNFATHNGSAKAGTDYTTATGTLNWGNHDGRIKTIRVALSRDTPFQGARTLTVT
                     VAKAAGGAALGDTTQTAVNVYGSVTNTTLGDAVRFLEQSSFGPTDGSIAAVRTLGIPA
                     YLDQQLAMPPTGYPGSAAVSADSGSGCPSGSSACLRDRYTTFPVATRFYRNALTGEDQ
                     LRQRVAWALSQIFVISGVKNSIAYGMRDYQQMLLADAFGNFRQLLNDVTLSPMMGYYL
                     DMVNNDKPNGDDNPNENYARELMQLFTIGTVMLNDDGTPKLDGKGRLIPTYDQDIIEG
                     FAHTFTGWTYPPAPGEAKQWTGTPWFRGDMPQIPAHHDTGSKELLRGTVLPAGQTQSQ
                     DLSGAVGNIFRHPNVGPFIGRQLIQFLVTSNPSPAYVSRITAVFNDNGSGVRGDLKAV
                     VRAILLDPEARGNVKKDAAYGKLREPVLFATGVLRGLGGTTQSDGQYPFEYCAWALDQ
                     NVFSPPTVFNFYPPDYPLQGTSLVGPPFGIYNTNTSVQRADFVNRLLTPPYAVAA"
     gene            2622..2977
                     /gene="ssrA"
                     /locus_tag="VHE37_15050"
     tmRNA           2622..2977
                     /gene="ssrA"
                     /locus_tag="VHE37_15050"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3032..3301
                     /locus_tag="VHE37_15055"
     CDS             3032..3301
                     /locus_tag="VHE37_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36907.1"
                     /translation="MQGKGFWIANNVVLLGLYAVTTWLCVSGNSHHWIVWLATIILAA
                     HALEIPLAFKVLKEKNPAPLRTVLGTFLFGFTYWLPAKRGIYAAR"
     gene            complement(3498..3582)
                     /locus_tag="VHE37_15060"
     tRNA            complement(3498..3582)
                     /locus_tag="VHE37_15060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3546..3548),aa:Leu,seq:taa)
BASE COUNT          621 a         1165 c         1255 g          715 t
ORIGIN      
        1 gcggccaccg cgtacggcgg cgtcagcagg cggttgacga agtcggcgcg ctgcaccgaa
       61 gtgttggtgt tgtagatgcc gaacggcgga ccgaccaggc tggtgccctg cagcggatag
      121 tcgggcggat agaagttgaa cacggtcggc gggctgaaca cgttctggtc gagcgcccag
      181 gcgcagtatt caaacggata ctggccatcg ctctgcgtgg tgccacccaa cccgcgcagc
      241 acgccagtgg cgaacagaac gggctcgcgc aacttgccgt aggccgcatc cttcttcacg
      301 ttgccgcgcg cttccgggtc gagcaggatc gcgcgcacca cggccttcag gtcgccgcgc
      361 acgccgctgc cgttgtcgtt gaacactgcg gtaatgcggc tgacgtaggc tggtgacggg
      421 ttgctggtga ccaggaactg gatcagctgc ctgccgatga aagggccgac gttgggatgg
      481 cggaagatgt tgcccaccgc acccgacagg tcctgcgact gcgtctgccc ggccggcagc
      541 accgttccgc gcagcagttc cttgctgccg gtgtcgtggt gtgccgggat ctgcggcatg
      601 tcgccgcgga accatggcgt accggtccat tgttttgcct cgcccggcgc cggtggatag
      661 gtccagccgg tgaaggtatg ggcgaagcct tcgatgatgt cctggtcgta ggtgggaatg
      721 agccggccct tgccgtcgag cttcggggtg ccgtcgtcgt tgagcatcac cgtgccgatg
      781 gtgaacaatt gcatcagttc gcgcgcgtag ttttcgttgg gattgtcgtc gccgttgggc
      841 ttgtcgttgt tcaccatgtc gaggtagtag cccatcatcg ggctgagcgt gacgtcgttg
      901 agcaactggc ggaagttgcc gaaggcgtcc gccagcagca tctgctggta gtcgcgcatg
      961 ccgtaggcga tgctgttctt cacaccggaa atcacgaaga tctgcgacag cgcccaggcc
     1021 acgcgctggc gcagctggtc ttcgccggtg agggcgttgc gatagaagcg cgtggcgact
     1081 gggaaagtgg tgtagcggtc gcgcaggcag gcggacgatc cgctgggaca gcccgagccg
     1141 ctgtcggccg ataccgccgc ggagccggga tacccggtcg gcggcatcgc cagctgctga
     1201 tccaggtagg ccgggatgcc caacgtacgc acggcggcaa tgctgccatc ggtcgggccg
     1261 aagctgctct gctcgaggaa gcggaccgca tcgcccagtg tcgtgttggt gacgctgcca
     1321 tatacgttca ccgcggtctg cgtggtgtca cccagtgcgg cgccgccggc cgccttggcc
     1381 acggtgacgg tcagcgtcct ggcgccctgg aacggcgtgt ccctgctcag cgcaacgcgg
     1441 atcgtcttga tgcggccgtc gtggttgccc cagttgagcg tgccggtggc ggtggtgtag
     1501 tccgtgcccg ccttggcgct gccgttatgg gtggcgaagt tcacgctgac tgcgccatcc
     1561 cagccgtcgg tgcgcgccac cgcgatctcg accgacccgt cggcctgggc agcgtccttg
     1621 gagggcgcaa ccatggaaat gttgccgggc tccggcagcc ggccggcgcc tgcaagaacg
     1681 atggcggtgg ggctgtgcgg cgcatcgctg gcgaccagca ggcgcgcctc caccttgccg
     1741 gtggccgttg cggtgaatct cacgctgagg ttgcatgaat gggtcgggcc gaccgggttg
     1801 ctgcaaccgt tgtgcaccag cgcgaactgg tcggcatgcg tgccggtgag gctgacgctg
     1861 cccacgtgca acggcaggtt gccgacgttg acgatgctgg tcgtctgcgt ggcggaggtg
     1921 tcgatcaaca gcgtcccgta atccagggtc gacggctggg ccgcggcgtc ggtctgcagg
     1981 ccgacgccgg tcagcgtggt cgtgtgcgga tttcccggtg cattgctggt taccgtgagg
     2041 ctggcggcgc ggtcgccacc agcccgcgga tggaaggtga tgctgaccgt gcaactggcc
     2101 cctgcagcca gcgtggcccc gcaggtcgtg gtcatcggga aatcgccggc gttggcgccg
     2161 gtgatgtgat tggccttgaa cgtcaacgcc gcgtcgccgg tgttgctgac cgtgaccgca
     2221 tgcacggtac tgtccgcacc gacgttgaca ttatggaagg cgataaaggt gggctgcagc
     2281 ttgagcaccg gcccggccag cgccgccccg ctccacagta tcaaggcagg cgcccacaag
     2341 cgccggaatc cctgcatcat gaccatgccc ccggtttcat ccatcgcggg cgcccggtgc
     2401 cgtgcaggcg tcgaacgcat gcagcccaaa ccccgcatct ccgcgactag tgtcactgcg
     2461 cccgccgcgt atgcgccgcc cgtccgtccg gccaaggcct ccgtccgccg gaccatggtc
     2521 gacaagccac cgagccgcgc cagcgcagcc attccctgaa aattccgctg ttccgcaccg
     2581 gggtcttgaa cgcgccgcgc gtgctaccat cattgctgtc tggggctgac atggcttcga
     2641 cggcgatgtt gaaacctgac gtgcatgccg agctgcggtg catctcgtaa aactggaccg
     2701 caaacaagct agttgcgaac gacgcgaact acgccctcgc cgcttaatcc ggcgagcgtc
     2761 tgaccggcag gtgttgacga ctccgaatca gatgtcgact ctcatcaact cgtggtgaag
     2821 cccgcccttg gccgatccgc taaatctcta aggggataag cgccggcgtt ccgtgcctgt
     2881 ccgggaaccg gcgaccaaaa ccaaagatcg ggctacgcat gtagagcgaa gggcggagaa
     2941 ttgtcggacg ggggttcaat tccccccagc tccaccaacc atgaaaacca cctgcgggtg
     3001 gttttcattt tttcggcacg gggagaacaa catgcagggc aagggattct ggatcgccaa
     3061 caacgtggtg ctgctcggcc tctacgccgt caccacctgg ctgtgcgtga gcggcaattc
     3121 gcaccactgg atcgtctggc tggccaccat catcctggcg gcgcacgcgc tggagatccc
     3181 gcttgcgttc aaggtgctga aggaaaaaaa tccggcgccg ctgcgcacgg tgctcggcac
     3241 cttcctgttc ggcttcactt actggctgcc ggccaagcgc ggcatctacg cggcgcgctg
     3301 agcgcgcgcg gaaatttttc gtggtgcccg aggccggact cgaaccggca tgaccttgcg
     3361 gtcgagggat tttaagttag gcagattcag caactaaatc atgttgttac atgacttagt
     3421 tttgtgaatc atgttttata ccgcctccga atatcaatgg tttaaggagg ggttttggga
     3481 atggttttag aggtgtttgg tgcgcccggc cggactcgaa ccggcacgcc tttcggccca
     3541 agattttaag tctcgtgcgg ctaccaatta cgccacgggc gcgcggcgat cagcatagcc
     3601 gaagccccgt gccaagagct cactcaggcg agaatgtgga agcctaattg gagattcctg
     3661 tcgatggcgg ctcaccatgc ttccactccg tcaccgtgca tcgcccgcgc gtgccgcact
     3721 tggtgcaccg gccgcgcctg gccagctcgg tcaacg
//
LOCUS       DASZZC010000710         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1212128,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000710 DASZZC010000000
VERSION     DASZZC010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212128"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VHE37_15065"
     CDS             complement(<1..1166)
                     /locus_tag="VHE37_15065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVT36908.1"
                     /translation="MIRLFSAPARTRFILAGACTLLLIGTVLGADDAATPETPIAAPH
                     TVIDLPQMGEPADLAMSPAQERALGAQVVAELYANNYVLEDPEVEEYISNIGWKLVSA
                     SLTKPPHVAFYPIADERINAFALPGGYIGVNAGILLSAANESELAGVMGHELAHVTQR
                     HIARSQEGETAASIATWAAVLAAILAGSGNPDVIIGALSLGQSVTYQRQVNYTRANEQ
                     EADRFGIQTMAAAGFDPQGMASFFTRLSQESRLYGNGLPEILRTHPLNTARVAEAQAR
                     IAALPKSNYKDSPDFAIIKARTRVLAADNASEAVEYFANIRETSVAARYGYAFALSLR
                     GQYDDAIAALQPALEQMPRQVNLNLLMSKLEGGLHQTEQQLATLDRTLRMYPGYP"
     gene            complement(1203..2084)
                     /gene="asd"
                     /locus_tag="VHE37_15070"
     CDS             complement(1203..2084)
                     /gene="asd"
                     /locus_tag="VHE37_15070"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186573.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HVT36909.1"
                     /translation="MTQHADTDASPRDLIFAAFQRILPTRFLSWCIYRLTRIESPGFK
                     NALIKYFLRGFTIDMTQAQVSDPYAYKSFNEFFTRPLKDGARQLAPEPAFVSPVDGTV
                     SRIGRIESGRLIQAKGHNYSATDLIGGGMDDVVPLLGGNYCTIYLAPYNYHRIHMPVA
                     GALRWWRYVPGRLFSVNPSTARALPGLFTRNERVIAMFDTERGPLAMVLVGALFVGGI
                     ETVWSGAVTPPHMRYEDKGSYMPLPKNGVRLARGQEMGRFNMGSTVILLTGQRSVNWA
                     TAVQAGSEVRMGQELGR"
     gene            complement(2193..2612)
                     /locus_tag="VHE37_15075"
     CDS             complement(2193..2612)
                     /locus_tag="VHE37_15075"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HVT36910.1"
                     /translation="MKKIVLLLLCCALPAWPCEGLTARDAWIREAPPGADVLGGYVSF
                     SNAGASEQSICHAQSAAFGSVEFHQMSMDHGQMQMRELDRLTVPAHGSLALAPGGTHL
                     MLMQPKAPLKAGQKVSIDFYCDGKEHLTVEFQVKSSS"
     gene            complement(2609..3214)
                     /locus_tag="VHE37_15080"
     CDS             complement(2609..3214)
                     /locus_tag="VHE37_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVT36911.1"
                     /translation="MSSRLTWIVAAVAGVAGLIAAALWFAPGPAALQSGVVLPQPRAL
                     GEFALVADDGQPFTLQRLRGHWTLIFPGFTHCPDVCPTTLALLKQVRVRLAGKPDVQV
                     VFLSVDPQRDTPQALSTYVHYFDPAFTGVTAAEPELSRVATQLAIAYEKVPGPSPDSY
                     SMDHSAALVLVNPQAQVAAYLSPPFQLETLAADLRKATGAS"
BASE COUNT          566 a         1078 c         1125 g          572 t
ORIGIN      
        1 gggtagcccg ggtacatgcg cagggtgcgg tccagcgtgg ccaactgctg ttcggtctgg
       61 tgcagtccgc cttccagctt gctcatcagc aggttgaggt tgacctgccg cggcatctgc
      121 tccagtgccg gctgcagcgc ggcgattgcg tcgtcgtact ggccacgcag cgagagggca
      181 aaggcgtagc cataacgcgc agccaccgag gtttcgcgta tgttggcgaa atattccacg
      241 gcctcgctgg cattgtcggc agccagcacc cgcgtgcgcg ccttgatgat ggcgaagtcc
      301 ggcgaatcct tgtagttgga cttgggcagc gccgcgatgc gcgcctgcgc ttcggccacg
      361 cgcgccgtgt tgagcggatg cgtacgcagg atttccggca ggccgtttcc gtagagcctg
      421 gattcctgcg acaggcgggt gaagaaactg gccatgccct gcggatcgaa gcccgccgcc
      481 gccatggtct gaatgccgaa gcgatcagcc tcctgctcgt tggcgcgcgt gtagttcacc
      541 tggcgctggt aggtcacgct ctggcccagc gacagtgcgc cgatgatcac atccgggttg
      601 ccggagccgg cgaggatcgc cgccagcacg gcagcccagg tggcgatact ggccgcggtc
      661 tcgccttcct gggaacgagc gatgtggcgc tgggtcacgt gggccagttc gtggcccatg
      721 acgccggcca gctcgctctc attggccgcc gacagcagga tgccggcatt cacgccgatg
      781 tagccgccgg gcagggcaaa ggcgttgatg cgttcgtcgg caatcgggta gaacgccacg
      841 tgcggcggct tggtcaggct ggccgatacc agcttccagc cgatgttgga gatgtactcc
      901 tccacttccg ggtcttcgag cacgtagttg ttggcgtaga gctcggcaac cacctgcgcg
      961 ccgagcgcgc gctcctgcgc cggcgacatg gccaggtccg ccggttctcc catctgcggc
     1021 aggtcgatca ccgtgtgcgg tgcggcgatc ggtgtttccg gcgtagcggc atcgtccgcg
     1081 ccgagcacgg tgccgatcag cagcagggtg caggcgccgg ccaggatgaa gcgcgtacgc
     1141 gcgggagcag aaaaaaggcg aatcatggcg ggcggtgctt ggcgttcagc ttggacggcg
     1201 actcagcggc cgagttcctg gcccatgcga acttcacttc cggcctgtac cgcggtggcc
     1261 cagttcaccg agcgctggcc ggtgagcagg atcacggtgg agcccatgtt gaagcggccc
     1321 atctcctgtc cgcgcgccag gcgcacgccg ttcttgggca gcggcatgta gctgcccttg
     1381 tcctcgtagc gcatgtgcgg cggcgtgacc gcgcccgacc acaccgtctc gatgccgccg
     1441 acgaacagcg cgccgaccag caccatggcc agcgggccgc gctcggtgtc gaacatggcg
     1501 atgacgcgtt cgttgcgcgt gaacagcccc ggcaaggcgc gggcggtcga aggattcacc
     1561 gagaacaggc gtcccggcac gtagcgccac cagcgcaatg cgccggccac cggcatgtgg
     1621 atgcggtggt agttgtacgg cgcgaggtag atggtgcagt agttgccgcc cagcagcggc
     1681 accacgtcat ccatgccgcc gccgatgaga tcggtggcgg agtagttgtg gcccttggcc
     1741 tggatcagcc ggccgctctc gatgcgcccg atgcggctca ccgtgccgtc caccgggctg
     1801 acgaacgccg gttcaggcgc cagctggcgc gcgccgtcct tgagcggccg ggtgaagaat
     1861 tcgttgaagc tcttgtaggc gtatgggtcg ctgacctgcg cctgcgtcat gtcgatggtg
     1921 aagccgcgca ggaagtactt gatcagcgcg ttcttgaatc ccggcgactc gatgcgcgtc
     1981 agccggtaga tgcaccacga aagaaagcgc gtgggcagga tgcgctggaa cgcggcgaag
     2041 atcaggtcgc gcggcgaagc gtcggtgtcg gcatgctggg tcatgcgtgc aaggctccca
     2101 ccgcaccggc attttgcttg ttataaatca ttatctatat ttaaaaacac tcaataaata
     2161 ataacaacag aaccggcggt gcggtgaacc cattatgaag agcttttcac ctggaattcg
     2221 accgtgaggt gttctttccc gtcgcagtag aaatcgatgc tcaccttctg cccggctttc
     2281 agcggcgctt tcggctgcat cagcatcagg tgcgtgccgc cgggcgcgag tgcgaggctg
     2341 ccatgcgccg gcacggtgag ccgatccagt tcgcgcattt gcatttgccc gtggtccatc
     2401 gacatctggt ggaactccac cgagccgaac gccgccgact gcgcgtggca gatgctctgc
     2461 tcggacgcgc cggcgttgga gaagctgacg tagccgccga gcacgtcggc gcccggcggc
     2521 gcctcgcgga tccaggcatc gcgcgcggtc aaaccctcac acggccacgc gggcagtgcg
     2581 cagcacaaca gcagcaggac aatttttttc acgaggcccc cgtggctttc ctgagatctg
     2641 cggccagcgt ttcaagctgg aatggcggcg acaggtaagc ggccacctgt gcctgcgggt
     2701 tgaccagcac cagggcggcc gagtgatcca tgctgtagct gtccggcgac ggccccggca
     2761 ctttctcgta ggcgatggcc agctgcgtgg ccacgcgcga cagctccggc tccgccgcgg
     2821 tcacaccggt gaaagcgggg tcgaagtaat gcacgtaggt ggaaagcgcc tgcggcgtgt
     2881 cgcgctgcgg atccacggac aggaacacca cctgcacatc gggcttgccc gccagccgca
     2941 cgcgcacctg cttgagcagg gccagcgtgg tcgggcacac gtccgggcaa tgggtgaagc
     3001 cggggaagat cagcgtccag tgtccgcgca ggcgctgcag cgtaaaaggc tggccatcgt
     3061 cggccaccag cgcgaactcg ccgagcgcgc gcggctgcgg caacaccacg ccggactgca
     3121 gcgcggccgg tcccggcgca aaccacagcg ccgcggcgat caagcccgcg acgcccgcca
     3181 ccgcggcgac tatccaagtc agtctggaag acacgagaat aggctccgtt cgttgccgct
     3241 gcccatcgcc gcaacgcata attgcgatta tctcacacgc acccgcgccg gccgcgcgca
     3301 ccgctgcatg tcacgcctca cagaaacctc ggtgctcgcc a
//
LOCUS       DASZZC010000711         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_381471,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000711 DASZZC010000000
VERSION     DASZZC010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381471"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2927)
                     /locus_tag="VHE37_15085"
     CDS             complement(<1..>2927)
                     /locus_tag="VHE37_15085"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HVT36912.1"
                     /translation="SAPGWSETGGPNVQPASAMKKIVYSETRVEGGKPFHGVLPMPPA
                     NFGPYQDVVRKPPFQINGEPKSTVPPLADTHWYADTRVIAYRLPAGEAMQQVARVSAS
                     AGTPDAGLLRDGKFDRAFVLPLAANGQDNWVQYDFGAPATVRSLRVGTRWSDGRASGL
                     SEGRVEASDDGVTFRNIAALPPAATSANLPLYTLALPETRARYFRLVIKPASGFSFPG
                     LPLRKFDQFQITELDFSSAARPNRYEAKAAYAVLADYDSAASVEVGADAAIDAGSVVD
                     LTAQLQPDGRLDWTPPSGEWNVIRFGAALTGQTNAPATSAGTGYEVDKLSAREGRNYL
                     DQYYGSLLAADGPLAGTHALQAILTDSWEAGQENWTPALLEEFRALRGYDATPYLPVL
                     ADHIVGSAAISDRFLWDFRRTLADMLAENHYKVIADYAHEHGLAYYGEAMGIDLPTTG
                     DGLQDKRYPTVPMGEFWQVAPDKPSNGNHIADVREAASAAHVYGQNIVATESFTGFPL
                     PGVPVPYSTTPWLLKPLADRFMTHGVNRFVIHTAVHQPLERGPGFTLSIFGQYFSRLE
                     TWGEQARGWMDYLARSSELLQQGRYAADIAYFYGEGAAATVPAGAVTDPAMPAGYGYD
                     FVCRDMLLKDFTVKDGRLLAPSGTSYRLLVLPASTTRMTLALVRRLREFVAAGAVLVG
                     PRPQGANGLADSDADVLAVASDLWGDLSAASKSARAYGKGRVYWSQPLADVLAAEKLA
                     PDIAFKIQGGSDKLISIHRHLDDGELYFIANQDAAAVRVDADLRVSGRAAELWHADDG
                     RIEAASFHQQSGRTVVPLALAPYEAVFVVLRRSTVARVHAERAERMQALGTIDGAWEV
                     SFTPDRGAPPVAHFDALASWSDNADAGVRYFSGTATYHKTMQVPKTWFEHGRRLLLDL
                     GAVHETAQVSINGRDAGIAWKPPYRVDVTGLLKPGANDVEIAVANLWANRFIGDQQPG
                     VQP"
BASE COUNT          469 a          972 c          998 g          488 t
ORIGIN      
        1 aggctgcacg ccgggctgct ggtcgccgat gaagcggttg gcccacaggt tggccacggc
       61 gatttccacg tcgttggcgc cgggcttgag caggcccgtg acatcgacgc ggtagggcgg
      121 tttccacgcg atgccggcat cacggccgtt gatgctcacc tgcgccgtct cgtgcacggc
      181 gccgagatcg agcagcaggc gacgcccgtg ctcgaaccaa gtcttcggca cctgcatggt
      241 cttgtgatac gtggcggtac cggagaagta gcgcacgccg gcatccgcgt tgtcgctcca
      301 cgatgcaagg gcgtcgaagt gcgcgacggg cggcgcgccg cggtccgggg tgaacgagac
      361 ttcccaggcg ccatcgatcg tgccgagcgc ttgcatccgc tccgcccgct ccgcatgcac
      421 gcgcgcgacg gtggagcgac gcagcaccac gaacacggct tcgtatggcg ccagtgccag
      481 cggcacgacc gtgcgcccgc tctgctgatg gaatgaggcc gcttcgatgc ggccatcatc
      541 ggcgtgccac aactctgcgg cacggccact cacacgcagg tccgcgtcga cccttaccgc
      601 tgcagcatcc tggttggcga tgaagtacag ttcgccatcg tcgagatggc gatggatcga
      661 aatgagcttg tcgcttccac cctggatctt gaacgcgatg tccggcgcga gtttttcagc
      721 ggccagcaca tcggccagcg gctggctcca gtacacgcgc cccttgccgt aggcgcgcgc
      781 ggatttgctg gcggccgaca gatcgcccca gagatcggac gcaacggcca acacgtcggc
      841 atcgctgtcg gccagcccgt tggcgccctg cggccgcggg ccgaccagca cggcaccggc
      901 tgcgacgaac tcgcgcagcc tgcgcaccag cgccagcgtc atgcgcgtgg tcgacgccgg
      961 cagcaccagc agccggtacg aggtaccgga cggtgccagc aggcggccat ccttcaccgt
     1021 gaaatccttg agcagcatgt cgcggcagac gaagtcgtaa ccgtaacccg ccggcatcgc
     1081 gggatcggtc accgcgcctg ctggcaccgt ggcagcggcg ccttcgccgt agaagtaggc
     1141 gatgtcggcc gcgtagcggc cctgctgcag cagttccgac gagcgcgcca ggtaatccat
     1201 ccagccgcgc gcctgctcgc cccaggtttc caggcgcgag aagtactggc cgaagatcga
     1261 cagggtgaat cccggtccgc gttccagtgg ctggtgcacc gccgtgtgga tgacgaaacg
     1321 gttgacgcca tgcgtcatga agcgatcggc caaaggcttg agcagccatg gcgtggtgct
     1381 gtacggcaca ggcacaccgg gcagtggaaa tcccgtgaag gattcggtgg cgacaatgtt
     1441 ctgtccgtag acatgtgcgg ccgatgccgc ttcgcgcacg tcagcgatgt gattgccatt
     1501 gctgggcttg tccggcgcca cctgccagaa ctcgcccatc ggcacggtgg ggtaacgctt
     1561 gtcttgcagg ccgtcgccgg tggtgggcag atcgatgccc atcgcctcgc cgtaatacgc
     1621 caagccatgt tcatgcgcgt agtcggcgat gaccttgtag tgattctccg ccagcatgtc
     1681 agccagcgtg cggcggaaat cccacaggaa gcggtcgctg atcgccgcgc tgccgacgat
     1741 gtgatcggcc agcaccggaa ggtagggcgt ggcgtcgtag ccgcggagag cgcggaactc
     1801 ctcgagaagt gcgggcgtcc agttttcctg gccggcttcc cagctgtcgg tgaggatggc
     1861 ctgcagcgcg tgcgtgccgg ccagcggtcc gtcggctgca agcagcgagc cgtagtactg
     1921 gtccagatag ttgcgccctt cgcgcgccga cagcttgtcc acttcgtaac cggtgccggc
     1981 agaggtcgcc ggcgcattgg tctggccggt gagcgcggca ccgaagcgga tcacgttcca
     2041 ctcgccggac ggcggcgtcc agtcgagccg gccatctggc tgcagctgcg cagtgagatc
     2101 aaccacgctg ccggcatcga tggcggcgtc cgcgccgacc tcgaccgatg ccgcagaatc
     2161 gtaatcggca agcaccgcgt aggccgcctt cgcctcgtag cgattcggac gcgccgcgct
     2221 ggagaaatcc agttcggtga tctggaactg gtcgaatttg cgcagcggca gcccggggaa
     2281 gctgaagccg gatgccggct tgatcaccag ccggaaataa cgcgcacgcg tctcgggcaa
     2341 ggccagcgta tagagcggca ggttcgcgga cgtcgccgcc ggtggcagtg cggcgatgtt
     2401 gcggaaggta acgccgtcgt cgctggcttc gacgcggcct tcggacaacc ccgatgctct
     2461 gccgtcactc cagcgcgtgc ccacgcgcag cgagcgcacg gtggccggcg cgccgaaatc
     2521 gtattgcacc cagttgtcct ggccgttggc ggcgagcggc agcacgaagg cccgatcgaa
     2581 ttttccgtcg cgcagcaggc ccgcatcggg cgtgccggcg ctggcgctca ctcgcgcgac
     2641 ttgctgcatc gcttcgcccg ccgggaggcg atacgcgatg acgcgcgtgt cggcgtacca
     2701 gtgcgtgtcg gccagcggcg gcacggtgga cttcggctcg ccgttgatct ggaacggcgg
     2761 cttgcggacc acgtcctggt acggcccgaa gttcgccggc ggcatcggca gcacgccgtg
     2821 gaacggcttg ccgccttcga cgcgtgtctc gctgtagacg atcttcttca tcgctgatgc
     2881 cggctgcaca ttcggcccgc cggtttcgct ccagcccggc gccgacg
//
LOCUS       DASZZC010000712         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_381584,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000712 DASZZC010000000
VERSION     DASZZC010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381584"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VHE37_15090"
     CDS             complement(<1..1460)
                     /locus_tag="VHE37_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family serine peptidase"
                     /protein_id="HVT36913.1"
                     /translation="MKLEVTGRARMMAMAAAASALIAAGPSQAAGWTHTATGVLAADA
                     TALRGEFSSGESVHIVVALAQRNVAELDSFVEEVNRPGSPAYGQFLTRGQYLERHAPT
                     QAQVNAVVAHLRNAGFVNIEVADNRMLVSADGTAGTVRRAFNAVLSHFDAGGRAAYAN
                     TTPVLVPQALSGIVVSVLGLQNIEQLHPTLVHAEGVMQPQATASAHGFNPTAFPTLYG
                     ASTLPTAAQTNVGVITEGNVAQTITDLRSFESKNALPQVPVSVVQVGAASTDTSGIDE
                     WNLDTQDIQAMAGGSVQQLLLYDAASMANADITAALNKVVVDHVAKVINVSLGECETS
                     AQSDGMVAAADAIFKAAVAQGQIFSISTGDSGSHECSVLIKTKSNTKQSYPAVSPYVV
                     AVGGTSLRATTAGAWASETVWSGTGGGPSTVEARPSWQTGVSGTARGVPDVAFDADPN
                     TGAIVVVGSSTSQIGGTSLAAPLFTGLWARIESANN"
     gene            1681..2349
                     /locus_tag="VHE37_15095"
     CDS             1681..2349
                     /locus_tag="VHE37_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVT36914.1"
                     /translation="MITVHHLNNSRSQRVLWLLEELGVPYEIRHYQRDAKTKLAPPEL
                     RAVHPLGKSPVITDGTVTVAESGAIVEYLVERYGNGRFIPPAGTPERLRYTYWMHYAE
                     GSAMLPLLLRLIFGFVASSPAPFFIKPVIKGIVSKVNAGFIDPQMKLHFDYVESELGK
                     SEWFAGSEITGADIQMSFPLEAAAVRGGGDRPRSKAFLERIHARPAYQRALQKGGPYE
                     LMGG"
     gene            complement(2363..3472)
                     /gene="dnaJ"
                     /locus_tag="VHE37_15100"
     CDS             complement(2363..3472)
                     /gene="dnaJ"
                     /locus_tag="VHE37_15100"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HVT36915.1"
                     /translation="MSKRDYYEVLGVAKDAGDDDLKKAYRRLAMKYHPDRNPGDHTAE
                     EKFKECNEAYEVLTDPRRRAVYDKHGHEGLQRGGGGGGDFGAGFADVFGDVFADIFGG
                     SRGGPRRGADLRYIMELTLEQAVFGSTESIRIPTQVDCEACGGHGTSNGKQPPECPTC
                     KGQGQVRVQQGFFVLQQTCPHCRGRGTLVIDPCRTCRGAGKVRKEKTLEVKIPPGVDT
                     GDRIRLNGEGEPGERTAPPGDLYVQINVKPHDFFERDGSDLFCTMPISIATAALGGEQ
                     QVPTLHGKETIHIGEGTQSGREFRLRGKGVKPVRGGAAGDLICTVMVETPVKLNKQQK
                     ELLRQFSESLGEGHHPQSKSWLDRARKFLDEHLKP"
     gene            complement(3469..4044)
                     /locus_tag="VHE37_15105"
     CDS             complement(3469..4044)
                     /locus_tag="VHE37_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HVT36916.1"
                     /translation="MGKGRLEAFSDGVIAIIITIMVLEMKVPHGSGLDALLPLVPVFL
                     TYVVSFVYVGIYWNNHHHLMHTVHEVSGGILWANLHLLFWLSLFPFATGWVGENHFDA
                     LPTALYGGVLMMAGVAYFILVQSIIRHHGRDSELARAVGNDFKGRISVVLYLAGIILA
                     FCLPWLASLVYVSVAAMWLVPDRRIENKIHR"
     gene            complement(4198..>4581)
                     /locus_tag="VHE37_15110"
     CDS             complement(4198..>4581)
                     /locus_tag="VHE37_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HVT36917.1"
                     /translation="VKDAEAHADEDKKFNELINARNQGDQMVHGVEKSLKDLGEQVQA
                     DEKKQIEDAIAALREVLKGDDKDAITAKSEALAQVSSKLMERVYAAKNAEAGAAGAGA
                     QAGAGEAKKDGDVVDAEFTEVKDKK"
BASE COUNT          729 a         1545 c         1478 g          829 t
ORIGIN      
        1 ttgttgttgg cgctctcgat gcgcgcccac aggccggtga acagcggcgc cgccaggctg
       61 gtgccgccga tctgcgaggt gctgctgccg acaacgacta tggcgccggt gttcggatcg
      121 gcatcgaagg cgacgtcagg cacgccgcgc gcggtgccgc tgacgccggt ctgccagctg
      181 ggcctggcct ccaccgtgct cgggccgccg ccggtgccgc tccacaccgt ttcgctggcc
      241 caggcgccgg cggtggtggc ccgcagcgac gtgccgccca ccgcaaccac gtacggcgaa
      301 actgccgggt agctctgctt ggtgttgctc ttggtcttga tcagcacgct gcattcgtgc
      361 gaaccggagt cgccggtgga gatcgagaag atctgcccct gcgccaccgc ggccttgaag
      421 atcgcgtcgg ccgccgcgac catgccgtcg ctctgcgcgc tggtttcgca ttcgcccagc
      481 gacacgttga tgaccttggc cacgtgatcc accaccacct tgttgagcgc agcggtgatg
      541 tcggcattgg ccatcgaagc ggcgtcgtac agcagcagct gctgcaccga accaccggcc
      601 atggcctgga tgtcctgggt gtcgaggttc cattcgtcga tgccgctggt gtcggtgctc
      661 gcggcgccca cctgcaccac cgagaccgga acctgcggca gcgcgttctt gctttcgaac
      721 gagcgcaggt cggtgatggt ctgcgcgacg ttgccctcgg tgatcacgcc gacgttggtc
      781 tgcgcggcag tcggcagcgt ggaggcaccg tacagcgtgg gaaacgcggt cgggttgaag
      841 ccatgcgccg aagcggtggc ctgcggctgc atcacgccct cggcatgcac cagcgtcggg
      901 tgcagctgct cgatgttctg caggccgagc accgatacga cgatgcccga cagcgcctgc
      961 ggcaccagca ccggcgtggt attggcgtag gcggcgcggc cgccggcgtc gaagtgcgac
     1021 agcaccgcat tgaacgcgcg gcgcacggtg ccggcggtgc cgtcggccga caccagcatg
     1081 cgattgtcgg ccacctcgat gttgacgaag ccggcattgc gcaggtgcgc caccaccgcg
     1141 ttgacctgcg cctgcgtcgg ggcgtggcgt tccaggtatt gcccgcgcgt caggaactgg
     1201 ccgtaggccg ggctgccggg gcggttcact tcctcgacaa agctgtcgag ctcggcgaca
     1261 ttgcgctggg ccagcgcgac cacgatgtgc acgctttcgc cggaactgaa ctcgccgcgc
     1321 agcgcggtag catctgcggc gagcacaccg gtggcggtat gcgtccagcc tgccgcctgc
     1381 gaggggccgg cggcgatcaa tgccgaagcc gcggcagcca tggccatcat ccgggcacgc
     1441 ccggtcactt cgagtttcat catgtcactc cctgttcgtg atgatcctga ccagcgaacc
     1501 ggcttgcagg cgggatcgat ggccggcgat gctactggtg gctgatgcgc agcaatacgg
     1561 caatggggag acggcaagcg tgaggttttt cctacgcaat gcgggcgccg ccatgcctat
     1621 gcggcacgcg cgtgacccgg cgcacatccg cacaacaacc atcacaaaca ggaggccctg
     1681 atgatcaccg tccatcacct gaacaattcg cgctcgcagc gcgtgctgtg gctgctggaa
     1741 gaactcggtg tgccgtacga gatccggcac taccagcgcg atgccaagac caagctggcg
     1801 ccgccggagc tgcgcgcggt gcacccgctg ggaaaatcgc cggtgatcac cgacggcacg
     1861 gtgaccgtcg ccgaatccgg cgccattgtc gagtacctgg tggagcgcta tggcaacggc
     1921 cgcttcattc cgcccgccgg gacgcccgag cgcctgcgct acacctactg gatgcactac
     1981 gccgagggct cggccatgct gccgctgctg ctgcggctga tcttcggctt cgtcgcctcg
     2041 agcccggcgc cgtttttcat caagccggtg atcaagggca tcgtcagcaa ggtcaatgcc
     2101 ggtttcatcg atccgcagat gaagctgcac ttcgactatg tcgaaagcga gctgggaaaa
     2161 tccgagtggt tcgccggcag cgagatcacc ggcgccgaca tccagatgag ctttccgctg
     2221 gaagccgccg cggtgcgcgg cggcggcgat cgcccgcgct cgaaggcctt cctcgaacgc
     2281 atccacgcgc ggccggcata ccagcgcgcg ctgcagaaag gcgggccgta tgaattgatg
     2341 ggcggatgaa accgggcttc agctagggct tcagatgctc gtcgagaaac ttgcgcgcgc
     2401 ggtcgagcca ggacttcgat tgcggatggt gcccttcgcc gagcgattcc gagaactgcc
     2461 gtagcagttc cttctgctgc ttgttcagct tcaccggcgt ctccaccatc acggtgcaga
     2521 tcagatcacc tgccgcgccg ccgcgcaccg gcttcacacc cttgccgcgc aggcggaatt
     2581 cgcggcccga ctgcgtgcct tcgccgatgt ggatggtctc cttgccgtgc agcgtcggca
     2641 cctgctgctc gccgccgagc gccgcggtag cgatggagat gggcatggtg cagaacaggt
     2701 cgctgccgtc gcgctcgaag aaatcatgcg gcttgacgtt gatctgcacg tagagatcgc
     2761 ccggtggcgc ggtgcgctcg cccggctcgc cctcgccgtt gaggcggatg cgatcaccgg
     2821 tgtcgacgcc ggggggaatc ttcacttcca gcgtcttttc cttgcgcacc ttgcccgcgc
     2881 cacggcaggt gcggcacggg tcgatcacca gcgtgccgcg cccgcggcaa tgcgggcagg
     2941 tctgctgcag cacgaagaag ccctgctgca cccgtacctg gccctggccc ttgcaggtcg
     3001 ggcactcggg cggctgcttg ccgttggacg tgccgtgacc gccgcaggct tcgcaatcga
     3061 cctgcgtggg aatgcggatc gattcggtgg aaccgaacac cgcctgctcc agcgtcagct
     3121 ccatgatgta gcgcaggtcg gcgccgcgcc gcggaccacc gcggctgccg ccgaagatgt
     3181 cggcgaacac gtcgccgaag acatcggcga agccggcgcc gaaatcaccg ccgccaccgc
     3241 cgccgcgctg caggccctca tgcccgtgct tgtcgtagac cgcgcgccgg cgcggatcgg
     3301 tcagcacttc gtaggcctcg ttgcactcct tgaacttctc ctcggcggtg tgatcgccgg
     3361 gattgcggtc cgggtggtac ttcatcgcca gccggcgata ggccttcttc aggtcgtcgt
     3421 cgccggcgtc cttggcgacg ccgagcactt cgtagtaatc gcgtttgctc atcggtggat
     3481 cttgttttcg atgcggcggt cgggcaccag ccacatggct gcgacgctca cataaaccag
     3541 gctggccagc cacggcaggc agaacgccag gatgatgccc gccaggtaca gcaccacgga
     3601 aatcctgccc ttgaaatcgt tgccgacggc gcgtgccagt tccgagtcgc gcccatgatg
     3661 ccggatgatg gactgcacca ggatgaaata ggcgacgccg gccatcatca gcacgccgcc
     3721 gtagagcgcg gtgggcagcg catcgaaatg gttctcaccc acccagccag tagcgaacgg
     3781 gaacagcgac agccagaaca gcaaatggag attggcccac aggatgccgc ctgacacctc
     3841 gtgcaccgtg tgcatcaggt gatggtggtt gttccagtag atgccgacgt agacgaagct
     3901 gaccacgtag gtcaggaata ccggcaccag cggcagcagc gcatccaggc ccgagccgtg
     3961 cggcaccttc atctccagca ccatgatggt gatgatgatg gcgatgacgc cgtcgctgaa
     4021 tgcctcaagc cgtcccttgc ccacctgctt cccctaaaac atcatgcccg cgaatgcggg
     4081 catgacatca cttccgtcat tcccgctcag gcgggaatcc agttttgatt tcatccggcg
     4141 tccatcgaac aactggatcc cgccttcgcg gggatgaggt tcgactggtg ccgaacgtca
     4201 cttcttgtcc ttgacctcgg tgaactcggc gtcgacgacg tcgccgtctt tcttcgcctc
     4261 gcccgcgccg gcctgcgcgc ccgcaccggc cgcacccgct tccgcgttct tggcggcgta
     4321 gacgcgctcc atcagcttgc tggaaacctg cgccagcgct tcgctcttgg cggtgatggc
     4381 gtccttgtcg tcgcccttga gcacttcgcg cagcgcggcg atggcgtcct cgatctgctt
     4441 cttctcgtcg gcctgcacct gctcgccaag gtccttcagc gacttctcca cgccgtggac
     4501 catctggtcg ccctggttgc gcgcgttgat cagctcgttg aacttcttgt cctcgtcggc
     4561 atgcgcctcg gcgtccttga c
//
LOCUS       DASZZC010000713         4680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_114740,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000713 DASZZC010000000
VERSION     DASZZC010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114740"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1137
                     /locus_tag="VHE37_15115"
     CDS             151..1137
                     /locus_tag="VHE37_15115"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HVT36918.1"
                     /translation="MSMRFSAGVLALAVATGAAAADGMGDLLVKMSQAAKTSNYQGVV
                     VYSGDDILETFRVIHRYADGSERERVQSLSGEPREVVNAGGKTICLLPKARRVTTEHP
                     TPKGLFPGLTAEKVALLTRLYDFEDLGTQRVAGRSCHGIAITPRDQLRYGYEVWADEA
                     SSVPLKVDLIGHTGQMLEQMLFTEVDFPQSIPDSAFAIDGEAPPAPALAAPPPPAPPP
                     AEAISLQGLPPGFRVLMRDERELPNGHGTVEHVLLSDGLSAISVFSTQQPSTTRPLQG
                     VQQMGAVHAYGRVVGAWRITVVGEAPPETVKMVGDGVKPPAAPAAPVPTPEK"
     gene            1473..>4680
                     /locus_tag="VHE37_15120"
     CDS             1473..>4680
                     /locus_tag="VHE37_15120"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HVT36919.1"
                     /translation="MKLPARSAPSASSRASDTHSLATLCRRIAAQRVPALSLAALGLA
                     FAPQAQAATITVTTSDDVTSATNGNCTILEAVRAAVEKQVVDQCPAGEVGVVDTVVLP
                     ANATFSFSSSYGHFAALPDIDGPVIISGNADVFERASGSTVEMHALVVNNYNPGHGGG
                     NLTISGVEIRNFEDHGSAAGMLVQGATLNAAGMNLHDLDGRGVMNDGGTLSLVNSVIS
                     RVADEGVLNQQGNTTLTGSGIDSSGSNGVRNYQGTLNLVDSTISGSGGAGIRNEHYSI
                     LQMNGGNVTDSHGWGVDNYFSVFGLTNATVSGGDDRGVVNRWGIGSLVQSTVTGNTGM
                     GVVQSGNPTYGPTQLYALQSTVSDNGDTGIKSLYSDLILSESTVSGNVASTPDYYYIT
                     AGIEVRGGTAGLVNSTIAGNAASVVDGTDVFGTVGGALFRSYAEVAMVNSTVSGNVGP
                     AGSDGYPVGGVYVAGYSVLATSNSIIDGNTSGGFADCATYAYGSIAGVYNNLSSDSSC
                     GFTITASANLGGLANNGGPTQTKALLPGSPALDSASGITPDIDQRGVARPQNGTGDLG
                     AYEYTSSAGPYTVTGGDTTPDAFSFAPVTNADLGVEVASNSIMVSGLSSPALATITPG
                     ASFTINGGPPVVGAWPLVTALVHDGDHIVVAQTSASSTHTKVSATLTIGGVSSTFDVT
                     TALKRDTTPDGFSIAPVGDADPGAAVSSAPVVASGFNTGVTATVDNGSFSVNGGPAVT
                     SATVHRNDSIVVTIDASSSFQGTASATLTIGSATGTFTVTTRAGSAIPTPFSFDPVTG
                     ATPGARVVSAPIVVSGLEVPASIGVSGDSSAQFCINDASSCYNNNLMTVSSGDLVYLR
                     LTASSSFSTQVSGTLDIGGVTGGFDVTTAAQDTMPDPIIFASRSNVPPNSVSTTAPRT
                     ITGINSPVNVSVSGDSSAVLLIDGNPVPGGSATGINAGQTVALRMTAAQAANAVASAT
                     LTVGGYSTTWSLTTRLDDVPKAFTFHSLTDVVGNRVHTTAGQKLVGVNAPVTVSVDGD
                     SSAVLLVDGAVVAGNSATVSSGQMIALQMTSSSTGGTKV"
BASE COUNT          833 a         1584 c         1543 g          720 t
ORIGIN      
        1 cgacgccatg ctgatggagc acagcaactc catcgccggc cagggcatgg gcatggggca
       61 gacgctgcgc tatgcacgct ttgccgcgca caccgcggaa taccgtccgg gcgcggatga
      121 cgcgcaggag caggccgggg atttccgctg atgtcgatgc gtttttccgc cggcgtcctt
      181 gcactcgccg tggccaccgg cgcggctgcg gccgacggca tgggcgacct gctggtgaag
      241 atgagccagg ccgccaagac cagcaattac cagggtgtgg tggtctacag cggcgacgac
      301 atcctggaga cttttcgcgt catccaccgc tatgccgacg gctccgagcg cgagcgcgtg
      361 cagtcgcttt ccggcgagcc gcgcgaggtg gtcaacgccg gcggcaagac catctgcctg
      421 ctgcccaagg cgcggcgcgt caccaccgaa catcccacgc ccaagggttt gtttcccggc
      481 ctgactgcgg aaaaagtcgc gctgctgacc aggctctacg acttcgagga tctgggcacc
      541 cagcgcgttg ccgggcgcag ctgccacggc atcgccatca cgccgcgcga ccagctgcgc
      601 tatggctatg aagtgtgggc cgacgaggcc agctcggtgc cgctgaaggt ggacctgatc
      661 ggccacaccg gccagatgct cgaacagatg ctgttcaccg aggtggattt cccgcagagc
      721 attcccgatt ccgcttttgc catcgatggc gaagcgccgc cggcgccagc gctggccgcg
      781 ccgccgccgc ccgcgccgcc gccagccgag gcgatcagcc tgcagggcct gccgccgggc
      841 ttccgcgtgc tgatgcgcga cgagcgcgaa ttgcccaatg gccacggcac ggtcgagcat
      901 gtcctgctct ccgacgggct gtcggccatt tccgtgttca gcacgcagca gccgtcgacc
      961 acgcgcccgc tgcagggcgt gcagcagatg ggcgcggtgc atgcctacgg ccgcgtggtc
     1021 ggcgcctggc gcatcaccgt ggtcggcgag gcgccgccgg aaacggtgaa gatggttggc
     1081 gacggcgtga agccgccggc agcgccggca gcgccggtgc cgacgccgga aaaatgatcg
     1141 tcggaccagg taccgcaacg ccgtagtagc tgttatttaa tatagtacaa atataaaaat
     1201 aatatatatt ttttaacaca atcatgccgg ggccggtgca ggcaaaaaac ggctctcctg
     1261 cgttctggcg cctgcttctg attccgtagc aacgctgtgc ctggccgcat tgccgtcggc
     1321 atgcgccatc gcccgtctgc gcgagcagtg gtcgacagtt tgcgcgcaga tgcgggtgct
     1381 agaatccggc cagaattttg cagcaggccg cgatcgtggg gatcggcggc cggaatcgca
     1441 gggcaggaag ccctcatccg tggggagctc tcatgaaatt gcccgccagg tcggcgccgt
     1501 ccgcatcgag ccgcgcgagc gacacgcatt ccctggcgac cctgtgccgg cgcatcgccg
     1561 cgcagcgtgt gccggcgctg tcgctcgccg cgctagggct ggctttcgcg ccgcaggcgc
     1621 aggcggcgac catcacggtg accaccagcg acgatgtcac cagcgccacc aacggcaact
     1681 gcaccatcct ggaagcggtg cgtgccgcgg tggagaagca ggtggtggac cagtgccctg
     1741 ccggcgaggt cggcgtggtg gacaccgtgg tgctgccggc caatgccacg ttttcctttt
     1801 ccagcagcta cgggcatttc gccgcgctgc cggacatcga cgggccggtg atcatctccg
     1861 gcaacgccga cgtattcgag cgcgcgagcg gttcaaccgt cgagatgcac gcgctggtgg
     1921 tcaacaatta caaccccggc catggcggcg gcaacctgac catcagcggc gtcgagatcc
     1981 gcaacttcga ggaccacggc agcgcggcag gcatgctggt gcagggggcg acactcaacg
     2041 ctgcagggat gaacctgcac gacctcgacg gtcgcggcgt gatgaatgac ggcggcacgc
     2101 tcagcctggt caactcggtc atcagccgcg tggcggacga aggcgtgctg aaccagcagg
     2161 gcaacaccac gctgaccggc agcggcatcg acagcagcgg gtccaacggc gtgcgcaatt
     2221 accagggcac gctcaacctg gtcgacagca ccatctccgg cagcggcggc gcgggcatcc
     2281 gcaacgagca ctactcgatc ctgcagatga atggcggcaa cgtcaccgac agccacggct
     2341 ggggcgtcga caactacttc agcgtgttcg gcctgaccaa cgccaccgtg tccggcggcg
     2401 acgaccgtgg cgtggtcaac cgctggggca tcggcagcct ggtgcagtca acggtgaccg
     2461 ggaataccgg catgggtgtc gtgcagtccg gcaatcctac ctatggcccc acccagctgt
     2521 atgcgctgca aagcacggtt tcggacaacg gcgacaccgg catcaagagc ctgtattcgg
     2581 acctgatcct cagtgaatcg acggtgtcgg gcaacgtcgc cagcacgccg gactattact
     2641 acatcaccgc cggcatcgag gtgcgcggcg gtacggcagg gctggtcaat tcgaccatcg
     2701 ccggcaatgc ggccagcgtg gtcgacggca ccgacgtgtt cggtacggta ggcggcgcgc
     2761 tgttccgcag ctatgccgag gtcgcgatgg tcaacagcac cgtgagcggc aacgtcgggc
     2821 cggccggttc ggacggctat ccggtgggtg gcgtctacgt ggctggctac tcggtgctgg
     2881 cgaccagcaa cagcatcatc gatggcaata cgtccggcgg ctttgcggat tgcgccactt
     2941 acgcttatgg gtccattgcg ggcgtctaca acaacctgtc aagcgactcc agctgcggat
     3001 tcaccatcac cgccagcgcc aatctcgggg gactggccaa caatggtggc cctacgcaga
     3061 ccaaggcgct tctgcccggc agcccggcgc tcgacagcgc cagcggcatc acgccggaca
     3121 tcgaccagcg tggcgttgcg cggccacaga acggcaccgg cgacctgggc gcgtacgagt
     3181 acacgtccag cgcgggaccg tacacggtga ccggcggcga tactaccccg gacgcattca
     3241 gcttcgcgcc ggtgaccaac gccgatctcg gcgtggaggt ggcgtcgaac agcatcatgg
     3301 tgtcgggcct gagcagcccg gcgctggcta ccatcacgcc cggcgccagc ttcaccatca
     3361 acggcggccc gccggtggtc ggtgcatggc cgctggtaac cgcgctggtg catgacggcg
     3421 atcacatcgt tgtcgcccag acctcggcat cgagcacgca caccaaggtc agcgcgacgc
     3481 tgaccatcgg cggcgtcagc agcaccttcg acgtcactac cgcgttgaag cgcgacacca
     3541 cgccggacgg cttcagtatc gcgccggtgg gcgatgctga cccgggggcc gcggtcagct
     3601 ccgcgccggt ggtggccagc ggcttcaaca ccggggtcac ggcgaccgtc gacaatggtt
     3661 cattcagcgt caacggcggc cccgccgtga ccagcgccac cgtgcaccgc aacgacagca
     3721 tcgtggtcac catcgatgcc tcgagcagtt tccagggcac cgcgtcggcc acgcttacca
     3781 ttggcagtgc gaccggcacg ttcaccgtga ccacgcgcgc gggcagcgca ataccgacgc
     3841 cgttctcgtt cgatccggtg accggcgcaa ctccgggcgc ccgcgtggta tcggcgccga
     3901 tcgtggtcag cgggctggag gtgccggcgt ccatcggcgt cagcggcgac agcagcgcgc
     3961 agttctgcat caacgacgcc agcagctgct acaacaacaa tctgatgacg gtgagcagcg
     4021 gcgacctggt ttacctccgg ctgaccgctt caagcagttt cagcacccag gtcagcggca
     4081 cgctcgacat cggcggcgtc accggcggct tcgacgtgac cacggcggcg caggacacga
     4141 tgcccgatcc gatcatcttc gccagccgca gcaacgtgcc gccgaactcc gtcagcacca
     4201 cggcgcccag gaccatcacc ggcatcaaca gcccggtgaa cgtctccgtg agcggtgaca
     4261 gcagcgctgt gctgctgatc gacggcaacc cggttcccgg cggctccgcg accggcatca
     4321 acgccggtca gaccgttgcg ttgcgcatga ccgcggcgca ggcagccaat gcagtcgcca
     4381 gcgccacgct caccgttggc ggctacagca ccacgtggag cctgaccacc aggcttgacg
     4441 acgtgcccaa ggcgttcacc ttccacagcc tgaccgatgt cgtgggcaat cgcgtgcaca
     4501 ccaccgccgg ccagaaactc gttggcgtca acgcgccggt cacagtatcg gtcgacggcg
     4561 acagcagcgc ggtgctgctg gtggacggtg cggtggtggc cggcaacagc gccaccgtca
     4621 gcagcgggca gatgatcgcg ctgcagatga cctcgagcag caccggcggc accaaggtga
//
LOCUS       DASZZC010000714         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_252542,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000714 DASZZC010000000
VERSION     DASZZC010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252542"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /gene="polA"
                     /locus_tag="VHE37_15125"
     CDS             <1..1939
                     /gene="polA"
                     /locus_tag="VHE37_15125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HVT36920.1"
                     /translation="FGRWLEELSATESVAPAGEPPPAAAKEETPVAQSPRTRAVCVTD
                     EPAFNDMLARLQSAPLVCFDTETTSLDSNSARLVGLAFAVDSGAGWYVPVGHDYLGAP
                     AQLDCNWVLQRLKPLLEDASKPKLGQHVKYDLNVLMCHGVQVRGVAHDTMLQSYVLDA
                     AGNRHDMDTLAEKYLGHQTIHFEDVAGKGKTQLTFNQVPLDRATEYSAEDADITLRLH
                     HVLYPRVCEVEALKRVYETIEMPLVPVLAQIEQTGVKVDGLLLRQISTELAQRMDELQ
                     AQCWKEAGGEFNLGSPKQLQAILYEKLQLPVLGKTPKGDPSTGEDVLEELAAQHPLPR
                     LILDWRALSKLRSTYTDNLPLSINAKTGRIHTSYGQAVASTGRLSSSDPNLQNIPVRN
                     AEGRRIRQAFIAEAGNALLSVDYSQIELRLMAHLSADERLMQAFHSGRDIHQATAAEV
                     FGLAPDAVTGEQRRAAKAINFGLIYGMSAFGLSKQLGIARGEASDYIERFFARYPGVK
                     QFMDGTRELSRKQGYVETLFGRRLYLPNINSRNAAVRQYAERTAINAPLQGTAADLIK
                     LAMIDLAGFLSENAPDVRMIMQVHDELVFEGPAAPLQELAPAIAERMCRISPLRVPLV
                     ADWGIAPNWDAAHTLGGSVKS"
     gene            complement(2241..2837)
                     /gene="yihA"
                     /locus_tag="VHE37_15130"
     CDS             complement(2241..2837)
                     /gene="yihA"
                     /locus_tag="VHE37_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HVT36921.1"
                     /translation="MRRNPFQQTAFLLSCASLEQLPADEQPEIAFAGRSNAGKSSALN
                     ALCGQKQLARVSKTPGRTQLINLFGVPGLGRLADLPGYGYAEVPREVSRSWGALIGGY
                     VETRANLRGLVVIMDIRHPLTDYDVQMLSWAGTRALHCHVLLTKADKLGFGAAKNQLL
                     KVKRELAERPVSVQLFSATSAQGVDEARSAIERLLRQA"
     gene            2970..3605
                     /locus_tag="VHE37_15135"
     CDS             2970..3605
                     /locus_tag="VHE37_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT36922.1"
                     /translation="MKRVLLACALCLPLMSFAQEAAVAKKDALTGGDAAAGAAKAATC
                     AACHGPTGNSSNPEWPKLAGQSSAYLVTQLKNFKSGARKQAVMMGMAGSLSDQDMKDV
                     AAHFASQKMEPGVASKDAVAVAEKLYRAGDAARGLPACAACHGPTGAGNAAAAYPRLG
                     GQHAKYVATQLTSFKSGERSIGANGQMMAAVAAKLTDTEIAALSSYVNGLQ"
     gene            3685..>4257
                     /locus_tag="VHE37_15140"
     CDS             3685..>4257
                     /locus_tag="VHE37_15140"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HVT36923.1"
                     /translation="MRLLSRAVIALAALLPLAAPAADAPNEEGKQYKLVMHPAPGPAN
                     AHVVVEEFFWYGCPHCYAFEPLLNDWLAKKPADVDFVRVPNTLGRAEGLLHSKLYYAL
                     EELNLLPKLHGAVFQAIHDERRPMSTEADLQAFVTDKGGVMPDVFSAAFNGNSVDAKV
                     RRAESLARTYGVASTPTLVVDGRWMLNATMA"
BASE COUNT          773 a         1401 c         1371 g          712 t
ORIGIN      
        1 cttcggccgc tggctggaag aactctctgc tacggaatcc gtagcgccgg cgggcgaacc
       61 gccgcctgcg gccgcaaagg aagaaacgcc ggtcgcacag tcgccacgca cgcgtgcggt
      121 gtgtgtcacc gacgagccgg cattcaacga catgctggcg cggctgcaat cggcgccatt
      181 ggtctgcttc gataccgaaa ccaccagcct ggattccaac agcgcgcggc tggtcggact
      241 ggctttcgcg gtggacagcg gcgcgggctg gtacgtgccg gtcggccacg attacctcgg
      301 agcgccggcc cagctcgatt gcaactgggt gctgcaaagg ctcaagccac tgctggaaga
      361 cgccagcaag cccaagctcg gccagcacgt gaaatacgat ctcaacgtgc tcatgtgtca
      421 cggcgtgcag gtgcgcggcg tggcgcacga caccatgctg cagagctacg tgctcgatgc
      481 ggcgggcaac cgccacgaca tggacacgct ggcggaaaaa tacctcggtc accagaccat
      541 ccactttgag gatgtggccg gcaagggcaa gacgcagctg accttcaacc aggtgccgct
      601 ggatcgcgcc accgaatatt ccgccgagga cgccgacatt accttgcgcc tgcaccacgt
      661 gctgtacccg cgcgtgtgcg aggtcgaggc gctcaagcgc gtgtacgaga ccatcgagat
      721 gccgctggtg ccggtgctgg cgcagatcga gcagaccggc gtgaaggtcg atggcctgct
      781 gctgcgccag atcagtacgg aactggcgca acgcatggac gagctgcagg cgcagtgctg
      841 gaaggaagcg ggcggcgaat tcaatctcgg ttcgcccaag cagctgcagg cgatcctgta
      901 cgagaagctg cagttgccgg tgctggggaa gaccccgaag ggtgacccat ccaccggcga
      961 agacgtgctg gaggagctgg cggcgcagca tcccctgccg cgtctgattc tcgactggcg
     1021 cgcgctgtcg aagctgcgct cgacctacac tgacaacctg ccgctgtcga tcaacgccaa
     1081 gaccggtcgc atccacacca gctacggcca ggccgtggca tcgaccggcc gcttgtcgtc
     1141 gtccgatccc aacctgcaga acatcccggt gcgcaacgcg gaagggcggc gcatccgtca
     1201 ggccttcatc gcggaagcgg gcaacgcgct gctgtcggtc gactattcgc agatcgagtt
     1261 gcgcctgatg gcacatctgt ccgctgacga gcgcctgatg caggcgttcc acagcggccg
     1321 cgacattcac caggccaccg ccgcggaagt gttcgggctg gcgccggacg cggtcaccgg
     1381 cgagcagcgg cgtgcggcca aggcgatcaa cttcgggctg atctacggca tgtcggcgtt
     1441 cgggctatcg aagcagcttg gcatcgcgcg cggcgaggcg agcgactaca tcgagcgttt
     1501 ctttgcgcgg tatccggggg tcaagcagtt catggacggc acgcgcgaac tgtcgcgcaa
     1561 gcagggttac gtggaaaccc tgttcggccg ccggctgtac ctgccgaaca tcaactcgcg
     1621 caacgccgcg gtccgccagt acgcggagcg caccgcgatc aatgcgccgc tgcagggaac
     1681 cgcggccgac ctgatcaagc tggccatgat cgacttggcc ggatttttgt cggagaacgc
     1741 gccggatgtg cgcatgatca tgcaggttca cgacgaactg gtattcgagg gccctgctgc
     1801 gccgctgcag gaactggcgc cggctatcgc cgaacgtatg tgccgtatat cccctctgcg
     1861 ggtaccgctt gtcgccgact ggggcatagc ccccaactgg gatgcggcgc ataccctcgg
     1921 cggctcggta aaatcctgaa gcggaaggga aaagagcgtt ggaaaacgct gactgattaa
     1981 aaagtaagca attgtaacct gttgaatttg cggcaaggat gccccaattt ccagccccgg
     2041 caagggcccg aacaaaaaaa gattggaact ttgggcggtc tcccgcctct aaccctgcga
     2101 gcgcggatta ctcccttttc ccgctccccg tctcactccc tggacgggtc cggcaccccc
     2161 aagccggagc acgttgcccg gtggttctcc cccgacccac cgggcacttt tatttccgcg
     2221 tctttttctg atcgaagcct tcaggcctgc cgtagcaacc gttcgatggc gctgcgcgct
     2281 tcatcaacac cctgcgcgct ggtggcggaa aacagttgca cgctgaccgg gcgttctgcg
     2341 agttcgcgct tcactttcag cagctggttc ttggccgctc caaagccgag cttgtccgcc
     2401 ttggtcagca gcacgtggca atgcagcgcg cgcgtacccg cccacgacag catctgcacg
     2461 tcgtagtcgg tgagcgggtg gcggatatcc atgatgacca ccagtccgcg caggttggcg
     2521 cgtgtctcca cgtagccgcc gatcagcgcg ccccagctgc ggctcacctc acgcggcact
     2581 tcggcataac cgtatcccgg caggtcggcc aggcggccaa gacccgggac gccgaacaga
     2641 ttgatgagct gcgtgcggcc gggcgttttc gacacgcgcg ccagctgctt ctgtccgcac
     2701 agcgcattga gcgcggacga tttgcccgcg ttggagcgcc cagcgaaggc aatttcaggc
     2761 tgctcgtcgg ccggcaattg ctcgagcgag gcgcaggaca gcaggaacgc cgtctgctgg
     2821 aaagggttcc tgcgcattgc tgcagcttta ccgggagagt tcaaggggat tcttcgccaa
     2881 acagcgccag tgtatgattc gcgcccacaa actaacggtt cacgcgcgct cccgccagcg
     2941 cgcggaatgt tcctctcggg agtcgtttga tgaagcgcgt tctgctggct tgcgcactgt
     3001 gcctgccgct catgtctttt gcccaagaag ccgccgtggc caagaaggat gccctgaccg
     3061 gcggcgatgc cgccgcgggc gcggccaagg ccgccacctg cgccgcctgc cacggcccca
     3121 ccggcaacag cagcaacccg gaatggccca agctggccgg ccagtcgagc gcatacctgg
     3181 tcacgcagct gaagaacttc aagagtggcg cgcgcaagca ggccgtgatg atgggcatgg
     3241 ccggttcgtt gtcggatcag gacatgaagg atgtcgctgc gcatttcgct tcgcagaaga
     3301 tggagccggg cgtggcgtca aaggacgccg ttgcggtggc ggaaaagctg tatcgcgccg
     3361 gcgacgctgc gcgcggcctg cccgcatgcg cggcctgcca cggcccgacg ggcgccggca
     3421 acgcggcggc ggcgtatccg cgcctgggtg gccagcacgc caagtacgtc gccacgcagc
     3481 tgacctcgtt caagagcggt gagcggtcca tcggcgccaa tggccagatg atggccgcgg
     3541 tggctgccaa gctgaccgac accgagattg ccgccctgtc ctcctacgtc aacggcctgc
     3601 aataagcctc ttaccgcggg atatcgttca ggccggcatc atcgtttgcg tctaagctgg
     3661 cggcggatcc acacggaaac tcgcatgcgc ctcctgtccc gcgcggtcat cgccctcgcc
     3721 gccctgttgc cgctggcggc acctgccgcc gacgcaccca acgaggaagg caagcaatac
     3781 aagctggtga tgcacccggc gccgggcccg gccaacgcgc acgtggtggt cgaggaattt
     3841 ttctggtacg gctgcccgca ttgctacgcc ttcgagccgc tgctcaacga ctggctggcg
     3901 aagaagccgg ccgatgtcga tttcgtgcgc gtgcccaaca cgctcggccg cgccgagggc
     3961 ctgctgcaca gcaagctgta ctacgcgctg gaagaactga acctgctgcc caagctgcac
     4021 ggtgccgtgt tccaggcgat ccacgacgag cgccggccga tgagcaccga ggccgatctg
     4081 caggcgttcg tcaccgacaa gggcggcgtg atgcccgacg tgttctccgc cgccttcaac
     4141 ggcaacagcg tcgacgccaa ggtgcgccgt gcggaatcgc tggcgcgcac ctacggtgtg
     4201 gccagcacgc ccacgctggt ggtcgacggc cgctggatgc tcaacgccac catggcc
//
LOCUS       DASZZC010000715         6698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_181362,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000715 DASZZC010000000
VERSION     DASZZC010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181362"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /gene="rlmJ"
                     /locus_tag="VHE37_15145"
     CDS             <1..472
                     /gene="rlmJ"
                     /locus_tag="VHE37_15145"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HVT36924.1"
                     /translation="EDAATLGALFAGDHQVRVIALDGWLAIGAQLPPKEKRGLVLVDP
                     PFEARDEFARVRELLEKSLARFAGGIYAVWYPLKNRHEADRFVRALAALDREALDIRF
                     DNGAQREGQMRECGMAIVHPPYQLEQALAPALAYLARALAQGGGAHYVMRRAAA"
     gene            578..784
                     /locus_tag="VHE37_15150"
     CDS             578..784
                     /locus_tag="VHE37_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36925.1"
                     /translation="MSASDSTSEKLYEGKFLILHKQGKWGAPEIDECPVDTRPGARPA
                     MDGRAEVGNRAKAAPWTAEVNRGS"
     gene            774..1304
                     /locus_tag="VHE37_15155"
     CDS             774..1304
                     /locus_tag="VHE37_15155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVT36926.1"
                     /translation="MEAEIRTLHQGQFLKLQREGRWEYVSRVNARGAGFIVALTPARE
                     LLLVEQYRVPLHVRCVELPAGIIGDSVEFSEESVEASAIRELEEETGWRGARARILCS
                     GPTAGGLTSELGHFVQVSELAHVHAGGGVDGEDITVHAVPLAQVAQWLDAQRARGLMV
                     DPRIYVGLYFIGAEKL"
     gene            1360..1746
                     /locus_tag="VHE37_15160"
     CDS             1360..1746
                     /locus_tag="VHE37_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT36927.1"
                     /translation="MTPHKPAGHNAATPYLIVNGADATVEFLKRVFGAAELLRMAGDG
                     GRIKHAEVRIDDSVTMLADPVPPGWPAVAAHVHVYVRDVDETYRKALAAGAVSVQEPA
                     RRGDEDKRGGVKDAGGTTWWIATRME"
     gene            1774..1986
                     /locus_tag="VHE37_15165"
     CDS             1774..1986
                     /locus_tag="VHE37_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36928.1"
                     /translation="MYIAIALLVAIAAVTAALLLRRRGSSSSTGGDDVFVAGTAEAGG
                     SDSGHCAPGDAGSSGDCGSADGGGAH"
     gene            complement(2013..3119)
                     /locus_tag="VHE37_15170"
     CDS             complement(2013..3119)
                     /locus_tag="VHE37_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT36929.1"
                     /translation="MNILRSLLSLLAAGLLAACGHSGGSAGNGTIGHVFIIVLENESY
                     SSTFGAGASSGAPYLTSVLPTQGALLTQYHATGHVSLDNYITMVSGQSPNVMTSSDCQ
                     IFLDWVGTQSPDSNGQVTGTGCVYPAAIKTVANQLQDAGYSWRGYMGDMGNDLARDGS
                     VDCSHPAVNSQDGTQSAEAADQYATRHDPFMYFHALIDDDANCKSHVVPLTQLPADLA
                     FTGTTPNYVFITPNLCDDGHDTGCANGDPGGLTSIDTFLQTWVPLITNSPAFQQDGLL
                     IVTFDEASPSDGSACCAEPTGPNTVVPGLIGPGGGRIGAVLISPLIRPGTVSDVPYNH
                     YAMLRTVEDIFGLQHLGYAGQNGLVPFGSDIFNR"
     gene            3197..5074
                     /gene="thiC"
                     /locus_tag="VHE37_15175"
     CDS             3197..5074
                     /gene="thiC"
                     /locus_tag="VHE37_15175"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVT36930.1"
                     /translation="MNAIPEDFLQAGSALAADVTRPFPASRKIYVGAARVPMREVAQT
                     DTPAGFGAQKNPALTLYDTSGPYTDPAARIDLTRGLAGLRAPWIAQRNDTEQLKAPSS
                     IFGRIRAADEKTRGLRFTHVRAPLRARAGANVSQMHYARRGVITPEMEYVAIRENCRL
                     EELREVYAKAGYLKRHAGKAFGARLPELVTPEFVREEVAAGRAIIPANINHPELEPMI
                     IGRNFRVKINANIGNSAVTSSIAEEVEKMVWSTRWGADTVMDLSTGKHIHETREWIVR
                     NSPVPIGTVPIYQALEKVNGKAEELTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYIPL
                     TAQRVTGIVSRGGSIMAKWCLAHHQENFLYTHFDDICEIMQAYDVSFSLGDGLRPGSI
                     ADANDEAQFGELHTLGELTKKAWARDVQVMIEGPGHVPMQLIKENMEEELKHCYEAPF
                     YTLGPLTTDIAPGYDHITSGIGAAQIGWYGCAMLCYVTPKEHLGLPDKDDVREGIVTY
                     KIAAHAADLAKGHPGAQLRDNALSRARFEFRWEDQFNLGLDPEKAKEFHDETLPQEGA
                     KLAHFCSMCGPHFCSMKITQDVRDYAAKQGLDEKSALDRGMAEKAAEFKQSGAEVYRK
                     L"
     gene            5258..6334
                     /locus_tag="VHE37_15180"
     CDS             5258..6334
                     /locus_tag="VHE37_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36931.1"
                     /translation="MNAARLKSFLLAIALAATSTGAMADDQGPAVADLSVAIAAQGKL
                     PDLNADEYFKGRGGGTFGKDIVTMLPNSKRVAVAGFRVVYVIESSASAHVRSSYLPGG
                     VEKTAAHSSIDVKLAGVDNATLQAITDKAYANFLEQLKAAGREVVPPEQLATLYPKLD
                     VAKTSADAPYQKDVGGKVGVAFSPTGVPLWWTVADSYGDVGPFNQTNMRAVPPFSAEA
                     NAIVIAPMIVVDFAKMGSSGNHSALINDEAKVDATLRMAVPVLTSGFTRAAETKGGLT
                     SSGDDAFFKLTKALVVDQEFATMETVEEEKSSKMLAFLTGSAKSSKKLTATTDNTRYS
                     AVASQVLNQATGALAKFFQQHPAQ"
     gene            complement(6422..>6698)
                     /locus_tag="VHE37_15185"
     CDS             complement(6422..>6698)
                     /locus_tag="VHE37_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36932.1"
                     /translation="ERTPAQAANDAADANAGTQILVPSPIGQGFSVLQASVVAQSDSG
                     KLLRFKDGTLKYIGNLAMLATPSPAVSAAANSDPAAVAAPPLAEPVH"
BASE COUNT         1190 a         2152 c         2297 g         1059 t
ORIGIN      
        1 cgaggacgcg gcaacgctgg gcgcgctgtt tgccggcgac catcaggtgc gggtgatcgc
       61 gctcgatggc tggctggcca tcggcgcgca gctccccccc aaggagaagc gcggcctggt
      121 gctggtcgat ccgccgttcg aggcacgcga tgaatttgcc cgcgtgcgcg aactgctgga
      181 gaaatcgctg gcgcgttttg ccggcggcat ctacgccgtg tggtatccgc tgaagaaccg
      241 ccacgaggcc gaccgcttcg tgcgcgcgct ggcggcgctg gaccgcgagg cactcgacat
      301 ccgcttcgac aacggcgcgc agcgcgaagg ccagatgcgc gagtgcggca tggccatcgt
      361 ccatccgccg tatcagctcg agcaggcgct ggcgccggcc ctggcgtatc tcgcgcgtgc
      421 gctggcgcag ggcggcggtg cgcactacgt catgcgcaga gcggcggcgt gagctgaaaa
      481 tggggtcacc gcatgccggt ttgtactgtt attaattatt gttcaattat taaattgatg
      541 tattttttgt aacatcaaaa atgcaggagc ggtgagcatg tccgcgagcg attccaccag
      601 cgaaaagctt tacgaaggca aatttctcat cctgcacaag caaggcaagt ggggcgcacc
      661 ggaaattgac gagtgtccgg tggacactcg tcccggcgcg cgcccggcca tggacggccg
      721 ggccgaggtg ggtaaccgcg cgaaagccgc gccatggacg gcggaggtga accgtggaag
      781 ctgagatcag gacgcttcat caagggcagt ttctgaaact gcagcgcgag ggccgctggg
      841 aatatgtgag tcgtgtgaac gcccgcggtg ccggcttcat cgtcgcgctg acgccggcgc
      901 gcgaactgct gctggtggag cagtaccgcg tgccgctgca cgtacgctgc gtcgaattgc
      961 cggccggcat catcggcgac agcgtggagt tctcggaaga aagcgtcgag gcttccgcca
     1021 tccgcgaact ggaagaggaa accggctggc gcggtgcgcg cgcgcgcatc ctgtgcagcg
     1081 ggccgaccgc gggcgggctg acctccgagc tcggccactt cgtgcaggtg agcgagctcg
     1141 cgcatgtgca tgccggcggc ggcgtcgacg gcgaggacat cacggtgcac gcggtaccgc
     1201 tggcgcaggt ggcgcaatgg ctcgatgccc agcgcgcgcg cgggctgatg gtcgatccgc
     1261 gcatctacgt gggcctctat ttcatcgggg ccgaaaaact gtagcgcggt tgtcgcgcca
     1321 ggccggcatc atcgtcacgt ccaccgggag ccagcaacca tgacgccgca caagcccgcc
     1381 ggccacaacg ccgccacgcc gtatctcatc gtcaacggcg cggacgcgac cgtggaattc
     1441 ctcaagcgag tgttcggtgc tgccgaactg ctgcgcatgg cgggtgatgg cggcaggatc
     1501 aagcacgccg aggtgcgcat cgacgacagc gtcaccatgc tggccgaccc ggtgccgccg
     1561 ggctggcccg ccgttgcggc gcatgtgcac gtctatgtgc gcgacgtgga cgagacctac
     1621 cgcaaggccc tggccgcggg cgccgtgtcg gtgcaggaac ctgcgcgccg cggcgacgag
     1681 gacaagcgcg gcggcgtgaa ggatgctggc ggcaccacct ggtggatcgc cacgcgcatg
     1741 gaatgacgct gcgcgaacga cggagggttg aacatgtaca tcgccattgc attgctggtc
     1801 gccatcgctg ccgtcaccgc cgcgctgctg ctgcgcaggc gcggttcgtc cagcagcacc
     1861 ggtggcgatg atgtcttcgt cgccggcacg gccgaggcgg gcggcagcga cagcggccac
     1921 tgcgcgccgg gcgacgccgg ctcgtcaggt gattgcggca gcgcggatgg cggtggcgcg
     1981 cactagcgcg ctccgcggcg gaccagtccg cttcagcggt tgaagatgtc gctgccgaac
     2041 ggcaccagtc cgttctggcc ggcgtaaccc aggtgctgca ggccgaagat gtcttccacc
     2101 gtgcgcagca tggcgtagtg gttgtacggc acgtcgctca ccgtgcccgg cctgatgagg
     2161 ggcgagatca gcacggcgcc gatgcgtccg ccgccggggc cgatcaggcc cggcaccacc
     2221 gtgttcgggc cggtgggctc ggcgcagcag gcgctgccgt cggaaggcga ggcctcgtcg
     2281 aaggtgacga tgagcagccc gtcctgctgg aacgccggcg agttggtgat cagcggcacc
     2341 caggtctgca ggaaggtatc gatcgaggtg agaccacccg gatcgccgtt ggcgcagccg
     2401 gtgtcgtggc cgtcgtcgca caggttgggc gtgatgaaca cgtagttggg cgtggtgccg
     2461 gtgaaggcca ggtccgccgg cagctgcgtc agcggcacca cgtgcgactt gcagttggca
     2521 tcgtcgtcga tcagcgcgtg gaaatacatg aacgggtcgt ggcgcgtggc gtactggtcg
     2581 gccgcctcgg cgctctgcgt gccgtcctgc gaattgaccg cggggtgcga gcagtccacc
     2641 gagccgtcgc gcgccaggtc gttgcccatg tctcccatgt agccgcgcca ggagtagccg
     2701 gcgtcctgca actggttggc caccgtcttg atcgccgccg gatagacgca gcccgtgccg
     2761 gtcacctggc cgttgctgtc ggggctctgt gtccccaccc agtcaaggaa gatctggcag
     2821 tccgaactgg tcatgacgtt gggtgactgg cccgacacca tggtgatgta gttgtccagg
     2881 ctgacatggc cggtggcgtg gtactgcgtg agcagcgcgc cctgcgtggg cagcacgctg
     2941 gtcaggtatg gcgcgccgct ggaggcgccg gcgccgaagg tgctggagta gctttcgttc
     3001 tccagcacga tgatgaagac gtggccgatg gtgccattgc cggcggaccc gccggaatgg
     3061 ccgcaggcgg cgagcaggcc ggcggccagc agcgacagca aggagcggag aatgttcatg
     3121 tgaacatcgt aatgcttcgg catgactccg gcgtgacgac ggcagggata aaatgccgcc
     3181 tcgtttcgag gacccgatga acgccatccc cgaggatttc ctgcaggccg gctccgcgct
     3241 cgccgccgac gtcacccggc cgtttccggc cagccgcaag atctacgtgg gcgccgcgcg
     3301 cgtgccgatg cgcgaagtgg cgcagaccga cacgccggcc ggcttcggcg cgcagaagaa
     3361 tccggcgctg acgctgtacg acaccagcgg cccctatacc gacccggcgg cgcgcattga
     3421 cctcacgcgc ggactggctg gtctgcgcgc gccgtggatc gcgcagcgca acgacaccga
     3481 gcagctgaag gcgccgtcgt cgatcttcgg ccgcatccgc gccgccgacg agaagacgcg
     3541 tggcctgcgc tttacccatg tgcgcgcgcc gctgcgtgcg cgcgccggcg ccaatgtgag
     3601 ccagatgcac tacgcgcgcc gcggcgtgat cacgccggag atggagtacg ttgccatccg
     3661 cgagaactgc cggctggaag agttgcgtga ggtttatgcg aaggccggct acctcaagcg
     3721 ccatgccggc aaggccttcg gcgcgcgcct gcccgagctg gtcacgccgg aattcgtgcg
     3781 cgaggaagtc gccgccggcc gcgccatcat tcccgccaac atcaaccacc cggaactgga
     3841 accgatgatc atcggccgta atttccgcgt gaagatcaac gccaacatcg gcaacagcgc
     3901 ggtcacctcg agcatcgccg aggaagtcga gaagatggtg tggtccacgc gctggggcgc
     3961 ggacacggtg atggatcttt ccaccggcaa gcacatccac gagacgcgcg aatggatcgt
     4021 gcgcaactcg ccggtaccca tcggcacggt gccgatctac caggcgctgg aaaaagtgaa
     4081 cggcaaggcg gaagaactga cctgggagat gttccgcgac acgctgatcg agcaggccga
     4141 gcagggcgtc gactacttca ccattcatgc cggcgtgctg ctgcgctaca tcccgctgac
     4201 cgcgcagcgc gtgaccggga ttgtctcccg cggcggctcg atcatggcca agtggtgcct
     4261 ggcgcatcac caggaaaatt tcctgtacac gcacttcgac gacatttgcg aaatcatgca
     4321 ggcgtatgac gtgtcgttct cgctgggcga cggcctgcgc ccgggctcga ttgccgatgc
     4381 caacgacgag gcgcagttcg gcgagctgca cacgctgggc gagctcacaa aaaaggcgtg
     4441 ggcgcgcgac gtgcaggtga tgatcgaagg tcccggccac gtgccgatgc agctgatcaa
     4501 ggaaaacatg gaagaggagc tgaagcactg ctacgaggct ccgttctaca cgctcggccc
     4561 gctgaccacc gacatcgcgc cgggttacga ccacatcacc tcgggcatcg gcgcggcgca
     4621 gatcggctgg tacggctgcg ccatgctgtg ctacgtcacg cccaaggagc acctgggcct
     4681 gccggacaag gacgacgtgc gcgaaggcat cgtcacctac aagatcgccg cgcacgcggc
     4741 ggacctggca aaaggccatc cgggcgcgca gctgcgcgac aacgcattga gccgcgcgcg
     4801 cttcgagttc cgctgggaag accagttcaa cctcgggctt gatcccgaga aggccaagga
     4861 attccacgac gagaccctgc cgcaggaagg cgccaagctc gcgcatttct gctccatgtg
     4921 cggtccgcat ttctgctcga tgaaaatcac ccaggatgtg cgcgactacg cggcgaagca
     4981 gggcctggac gaaaaatccg cgctggaccg cggcatggcg gagaaagcgg cggagttcaa
     5041 gcagtccggc gccgaggttt accgcaagct gtgaaatctc cccggcgctg cgcgccgacc
     5101 ctcgctgtgc cgtcctggca agaacgcgcc atccatggcg cgcactttca atagggtcgc
     5161 gccgaaggcg cggggagatt cgacccccca tctagggggc gcgccgcgcg gctgcacatt
     5221 cctaccatcg tcccatcgac acgcatggga gttggacatg aacgcagcac gcctgaaatc
     5281 cttcctgttg gcaattgcgt tggccgcgac cagcaccggc gccatggccg acgatcaggg
     5341 ccctgcggtg gccgatcttt cggtcgccat cgccgcgcaa ggcaagctgc ccgacctcaa
     5401 tgccgacgag tacttcaagg gccggggcgg cggcaccttc ggcaaggaca tcgtcaccat
     5461 gctgcccaac agcaagcgcg tggcggtggc gggtttccgc gtggtctacg tgatcgagag
     5521 cagcgccagc gcgcacgtgc gcagcagcta cctgccgggc ggcgtggaga agaccgccgc
     5581 gcattcctcc atcgacgtca agctggccgg cgtcgacaac gccacgctgc aggccatcac
     5641 cgacaaggcc tatgccaact tcctcgagca gctgaaggcg gccggtcgcg aggtggtgcc
     5701 gccggagcag ctggccacgc tctaccccaa gctcgacgtg gccaagacca gcgccgacgc
     5761 gccgtaccag aaggacgtgg gcggcaaggt tggcgtcgcg ttttcgccca ccggagtgcc
     5821 gctgtggtgg acagtggccg attcctacgg cgatgtcggc ccgttcaacc agaccaacat
     5881 gcgcgcggtg ccgccattct ctgccgaagc gaatgcgatc gttatcgcgc cgatgatcgt
     5941 ggtcgacttc gccaagatgg gttcctcggg caaccactcg gcgctgatca acgacgaggc
     6001 caaggtcgac gccacgctgc gcatggcggt gccggtgctg accagcggct tcacccgtgc
     6061 cgccgagacc aagggcggcc tcacctcgtc gggcgacgac gccttcttca agctgaccaa
     6121 ggcactggtg gtcgatcagg aattcgccac catggaaacg gtggaagaag agaagagcag
     6181 caagatgctg gcattcctca ccggctcggc aaagagcagc aagaagctca ccgccaccac
     6241 cgacaacacg cgctacagcg cagtggccag ccaggtgctg aaccaggcca ccggcgcgct
     6301 ggcgaaattc ttccagcagc atccggcgca atagcggtgc ggtcgctgtg cgcccggccg
     6361 gttcgttcct atcgcaggcc agcgtggatt gaatgacacg ccgccgctgc atgcttgctg
     6421 ttcaatgcac gggctcggca aggggcggcg cggcaaccgc ggcaggatcc gaattggccg
     6481 ctgcactgac tgccggcgag ggcgtcgcca gcatggcgag attgccgatg tacttgagcg
     6541 tgccgtcttt gaagcgcagc agcttgccgc tgtctgactg cgcgacgacg gaggcctgca
     6601 acacgctgaa gccctgacca atgggcgagg gcaccaggat ctgagtgccg gcgttggcat
     6661 cagcagcgtc atttgccgcc tgtgccggcg tccgttcg
//
LOCUS       DASZZC010000716         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_382060,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000716 DASZZC010000000
VERSION     DASZZC010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382060"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VHE37_15190"
     CDS             <1..921
                     /locus_tag="VHE37_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36933.1"
                     /translation="ENDSLRLDYAQIDYGFLSGNGYNAGIRAGRVKNPFGLFNETRDV
                     VFTRPGILLPESIYLDREGLRNLLFSSDGGQLYGGINIGDHYSSVVITHGLNRNFTDL
                     QKRVLTGGLNLPADVELSDFYVGRVEDESNGGVWRNAFSFVHGDIDIMPNPGAPVSGH
                     LGANFFVLSSRYNGERWSATGEYSLIYTRGNYSLTGPIDSKSDGGYLQADYRFNPQWS
                     LMTRYEMSFSNRNDRDGRQYASATGGDRYSQFSRDATLGLKWLPDQHWGVWGEYHLID
                     GTSSVSPLDNVGRAPDDHWSMLLLMGAYRF"
     gene            933..>2959
                     /locus_tag="VHE37_15195"
     CDS             933..>2959
                     /locus_tag="VHE37_15195"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT36934.1"
                     /translation="MAPHSASTAEPAAAPAFGMSLKWKAFLVLLSVLSVIHLSLGYLG
                     YRKLVDQNEEHSRERLASYPGIVTALLEESQQNLTRTGAQLAASVSVEALAQGRGPAD
                     SQLSLELLASLSDIQYFDAQGRPLGAAAPAPPDHATLAAVVQGHRPLAHLHCAEECVQ
                     RVFVPAFDLQGREIVVMMARPLSDTLLAFRKLTGADVAVLAADSAQAVAAPALWGRRI
                     MAVTRAPEMLPQLESVGGQLNAAALPGTPFRAGSRSTHLAMQLTPPDAVAGAQFLFVV
                     DESSELQRIQATVTNGVIVTAMGLVISACALFLMLTPAMRRLTRVTQALPLLAQQRFD
                     SARQLILASDRRASGFADEIDLLNRAALRLADRLQKLNAAEASNEAKSSFLAAMSHEI
                     RTPMNGVLGMLELFEHSRLDNEQREAISVIRDSAQALLRVIDDILDFSKIEAGRIDLE
                     EIPFSLAEVIEGTAETLAPGALAKQVKIFVYVAPDLPAQLCGDPVRLRQVLFNLCGNA
                     IKFTREGRVVVRAERVGGDDGKCRLHCSVSDSGIGIEPAALQRLFEPFRQAESSTTRR
                     YGGSGLGLSICKGLIQRMGGVIGCNSQPGRGSEFWFELELPVAAHQGDDRLADVLRRT
                     SVHVATAQVDESLMLISYLQAAGATLASAEVAAVNITEQCIDPQQPTLRPAL"
BASE COUNT          466 a         1038 c         1002 g          453 t
ORIGIN      
        1 gagaacgaca gcctgcgcct ggactatgcg cagatcgact acggcttcct tagcggcaac
       61 ggctacaacg ccggcatccg cgccggccgg gtgaagaacc cgttcggcct gttcaacgaa
      121 acgcgcgacg tggtgttcac ccgtcccggc atcctgctgc cggaatcgat ctacctggac
      181 cgcgaagggc tgcgcaacct gctgttctcg tccgacggcg gccagctgta cggcggcatc
      241 aacatcggtg accactacag cagcgtggtg atcacgcacg ggctcaaccg caacttcacc
      301 gacctgcaga agcgcgtgct gaccggcggc ctcaacctgc cggccgacgt cgaactgtcg
      361 gacttctacg tcgggcgcgt cgaggacgag agcaacggcg gcgtctggcg caacgccttc
      421 agcttcgtgc acggcgacat cgacatcatg cccaacccgg gcgcgccggt cagcggtcac
      481 ctgggcgcca atttcttcgt gctgtccagc cgctacaacg gcgagcgctg gagcgccacc
      541 ggcgaataca gcctgatcta cacgcgcggc aactacagcc tgaccgggcc gatcgacagc
      601 aagtcggacg gcggctacct gcaggccgat taccgcttca acccgcagtg gtcgctgatg
      661 acgcgctatg aaatgtcgtt ctccaaccgc aatgaccgcg acggccgcca gtacgccagc
      721 gccaccggcg gcgaccgcta cagccagttc tcgcgtgacg ccactctcgg cctgaagtgg
      781 ctgcccgacc agcactgggg cgtgtggggc gaatatcacc tgatcgacgg cacctcgtcg
      841 gtgtcgccgc tggacaacgt cggccgcgcg cccgacgacc actggtcgat gctgctgctg
      901 atgggcgcct accgcttctg agcggcagcg ccgtggcgcc ccattccgcc agtaccgccg
      961 agccggccgc ggcgccggct ttcggcatga gcctcaagtg gaaggcattc ctggtgctgc
     1021 tgtcggtgct gagcgtgatt catttgtcgc tcggctacct cggctaccgc aagctggtcg
     1081 accagaacga ggaacactcg cgcgagcggc tggcctcgta cccgggcatc gtcacggcgc
     1141 tgctggagga atcgcagcag aacctgaccc gcaccggtgc gcagctggcg gcatcggtca
     1201 gcgtggaggc gctggcgcag ggccgcgggc cggccgattc gcagctgtcg ctggaactgc
     1261 tggccagcct cagcgacatc cagtatttcg acgcccaggg ccgcccgctg ggcgccgccg
     1321 cgccggcgcc gccggatcac gccacgctgg cggcggtggt gcagggccac cggccgctgg
     1381 cgcatctgca ttgcgccgag gagtgcgtgc agcgcgtgtt cgtgccggcc tttgacctgc
     1441 agggtcgcga gatcgtggtg atgatggcgc ggccgctgtc cgacacgctg ctggccttcc
     1501 gcaagctcac cggcgccgac gtcgccgtgc tggccgctga cagcgcccag gccgtggccg
     1561 cgccggcgct gtggggcagg cgcatcatgg cggtgacgcg cgcgccggaa atgctgccgc
     1621 agctggaatc cgtcggcggc cagctcaacg ccgccgccct gcccggcaca ccgttccgcg
     1681 ccggctccag gtccacccac ctggccatgc agctgacacc gccggacgcg gtcgccggcg
     1741 cgcagttcct gttcgtggtg gatgaaagca gcgaactgca gcgcatccag gccaccgtga
     1801 ccaacggcgt gatcgtcacc gccatgggcc tggtcatctc ggcctgcgcc ctgttcctga
     1861 tgctgacgcc ggccatgcgc cggctgacgc gggtaacgca ggcgctgccg ctgctggcgc
     1921 agcagcgctt cgacagcgcg cgccagctga tcctggcttc ggatcgccgc gccagcggtt
     1981 ttgccgatga gatcgacctg ctcaaccgcg ccgcgctgcg cctggccgac cgcctgcaga
     2041 aactcaacgc ggcagaggcc tcgaacgaag ccaagtccag cttcctcgcg gcgatgagcc
     2101 acgagatccg cacgccgatg aacggcgtgc tcggcatgct ggaactgttc gagcactcgc
     2161 ggctggacaa cgagcagcgc gaggcgatca gcgtgatccg cgattcggcg caggcgctgc
     2221 tgcgcgtgat cgacgacatt ctcgatttct ccaagatcga agccggacgc atcgacctcg
     2281 aggagattcc cttctcgctg gccgaggtga tcgagggcac cgccgagacg ctggcgcccg
     2341 gagcgctggc caagcaggtg aagatcttcg tgtacgtggc gcccgacctg ccggcgcagc
     2401 tgtgcggcga cccggtgcgg ctgcgccagg tgctgttcaa cctgtgcggc aacgcgatca
     2461 agttcacccg cgaaggccgc gtggtggtgc gcgccgagcg cgtcggcggc gacgacggca
     2521 agtgccgcct gcactgcagc gtgagcgaca gcggcatcgg catcgagccg gccgcgctgc
     2581 agcggctgtt cgagccgttc cgccaggcag agtcgtccac cacgcgccgc tacggcggct
     2641 cggggctggg cctgtcgatc tgcaaggggc tgatccagcg catgggcggc gtcatcggct
     2701 gcaacagcca gccgggccgc ggcagtgagt tctggttcga gctggaactg ccggtggcgg
     2761 cgcaccaggg cgacgatcgc ctggccgatg tgctccgccg caccagcgtg catgtcgcca
     2821 ctgcgcaggt ggacgagtcg ctgatgctga tctcctacct gcaggcggcc ggcgccaccc
     2881 tcgcttcggc ggaagtggcg gcggtgaaca tcaccgagca atgcatcgat ccgcagcagc
     2941 cgacgctgcg cccggcgct
//
LOCUS       DASZZC010000717         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_382186,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000717 DASZZC010000000
VERSION     DASZZC010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382186"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VHE37_15200"
     CDS             complement(<1..1062)
                     /locus_tag="VHE37_15200"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HVT36935.1"
                     /translation="MALYQPSSLEQSLITVNRAAELAWAQQRGGLMFRLEAGESLDGG
                     IPDLSLEGAQRRTRLGEEIAALLATVDDEKLPHEFALTSKLLRYYAAAWVQDAERYWL
                     ACDPTGSLFYGPFAQTAYTGGYLFSFIGRALAQFRFEKDGDGDRYLVLLSDITRLLRQ
                     IHARTAGQAERGILIHKLQLPAVRNLLAALRAGAAAGYPVALVRLSKLKEAGAVAAEI
                     QRRVEQQVLPAFDALTAQLDHDYERQAPEGVGMDKLPGGKTVYDELVRLHTTMNLSPA
                     QVHAAGHARMARIEAQMAEVRNKLGFKGSAAEFHLNLRKQPGAVAKTPDEIGARMRRH
                     KDRVEQRFDEFFAQRSAFEY"
     gene            complement(1089..1490)
                     /locus_tag="VHE37_15205"
     CDS             complement(1089..1490)
                     /locus_tag="VHE37_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36936.1"
                     /translation="MSKLVFMSQAHVARMNELLAADAASRAACAGLAHRWDMVYELQH
                     GTHTVWWTMSFDPAYGVSFSLEAPRRAAQILFRGEYKAMLDWMRLRKAGGGETPEPVT
                     MSGDSGGMSIIGPAYQAAAQAATLDTEIPQV"
     gene            complement(1487..>3415)
                     /locus_tag="VHE37_15210"
     CDS             complement(1487..>3415)
                     /locus_tag="VHE37_15210"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HVT36937.1"
                     /translation="ASGSAFKAPAPNALDTYLGMTVEARTTPRPFKRSTPLVDPTDAY
                     EVPSPDGNWMLSIQNHNVALRATVDGRSTQLTHDGIPAVEWNIDWTNPMYVMMGLGAP
                     ATNWSPQGNRIAAYRVDYRGVVQAPQVHYLKRNDEVVYRNFCKAGGVLEKLSLYVLDV
                     AGRAPVEIQLGDTRDKYPCFAGWLPDGSELLVFVMSRDCRRVEIYAANAVSGATRLLF
                     TEEGATFVRIHHDIYYGRKTGLTLTPDGRHLLWLSERDGWKHIYQYDLNGKPVAQLTQ
                     GDWAVCDVTRVIGNDVYFTAHTDTARPYDVHLCRVPLGGGAVKVLTEGKGKHACQIAP
                     HGQVFLDTRSTPSTPPVTSLRRIDGTLLNAEVSKADISRLQQVGFAEPEEFCVKAADG
                     STDLWGVIYKPHDFDPKKKYPVIEYIYGGPQMAVADHGFAAVALSMGGMALRVAQYGY
                     VVVMLDACGTPERSKAFHDACYGSYCGTLAADHAAALRNLAAKYSYIDLGRVGIMGGS
                     WGAYSSFRCLAEHPDIYKAAVPFAPGFDPYSCVLYECYLGLPQDNPAGYRKADPYPLA
                     AQLKGEVMLAGGTSDHATWTDALKMSEALIRAGKLHQFVVLPGQYHGFDSVHDDYFHR
                     CFSAFFEQHVKNRKADGA"
BASE COUNT          547 a         1122 c         1162 g          584 t
ORIGIN      
        1 gtactcgaaa gcggaacgct gcgcgaagaa ctcgtcgaag cgctgctcca cgcgatcctt
       61 gtgccggcgc atcctggcgc cgatctcgtc gggcgttttc gccacggcgc cgggctgctt
      121 gcgcaggttg aggtggaact cggccgcgct gcccttgaag cccagcttgt tgcgcacctc
      181 ggccatctgc gcctcgatgc gcgccatgcg cgcgtggccg gccgcatgca cctgcgccgg
      241 gctgaggttc atggtggtgt gcaggcgcac cagctcgtcg tagaccgtct tgccgcccgg
      301 cagcttgtcc atgcccacgc cttcaggcgc ctggcgctcg tagtcgtggt ccagttgcgc
      361 ggtcagcgcg tcgaacgccg gcagcacctg ctgttccacg cggcgctgga tttccgcggc
      421 aacggccccg gcttccttca gtttcgacag gcgcacgagc gccacgggat aaccagccgc
      481 ggcgccggcg cgcaaggccg ccagcaggtt gcgcaccgcc ggcagttgaa gcttgtggat
      541 gaggatgccg cgctcggcct gccctgcggt gcgcgcgtgg atctggcgca gcaggcgcgt
      601 gatgtcggac agcagcacca ggtagcggtc gccatcgccg tccttctcga agcggaactg
      661 tgcgagcgca cggccgatga agctgaacag ataaccgccg gtgtaggcgg tctgggcaaa
      721 tggaccgtag aacaggctgc cggtcggatc gcaggccagc cagtaacgct ccgcatcctg
      781 gacccacgcg gcggcgtagt agcgcagcag cttgctggtc agcgcgaatt cgtgcggcag
      841 cttttcgtca tcgaccgtgg ccagcaaggc ggcgatctcc tcgcccagcc gggtgcggcg
      901 ctgcgcgcct tccaggctga ggtcgggaat tccaccgtcg agcgactcgc ccgcttccag
      961 gcggaacatc agcccgccgc gctgctgcgc ccacgccagc tcggcggcgc gattgaccgt
     1021 gatcagggac tgttcgaggg atgaaggctg gtacagcgcc atggcggact ccgtgaaagc
     1081 gggtgaattc agacctgcgg aatttcggtg tcgagggtcg cggcctgcgc agccgcctgg
     1141 tacgcgggcc cgatgatgct catgccgccg gagtcgccgg acatggtcac gggctccggc
     1201 gtctctccgc ctcccgcctt gcgcaggcgc atccagtcga gcatggcctt gtactcgccg
     1261 cggaacagta tttgcgcggc gcgcctgggg gcttcgaggc tgaaggagac gccataggcc
     1321 ggatcaaagc tcatggtcca ccacacggtg tgcgtgccgt gctgcagctc gtagaccata
     1381 tcccagcggt gtgcgagccc ggcgcaggcg gcgcggctcg cagcatcggc ggccagcagt
     1441 tcattcatcc gcgcaacatg cgcctgcgac atgaacacca gcttgctcat gcaccgtccg
     1501 ccttgcggtt cttcacgtgc tgctcgaaga aggccgagaa gcagcgatgg aagtaatcgt
     1561 cgtgcaccga gtcgaaacca tggtactgcc cgggcagcac cacgaactgg tgcagcttgc
     1621 cggcgcggat cagcgcctcc gacatcttca gcgcatcggt ccaggtggcg tggtcggacg
     1681 tgccgccggc cagcatcacc tcgcccttca gctgcgcggc gagcgggtac gggtcggcct
     1741 tgcggtagcc cgcggggttg tcctgcggca ggcccaggta gcattcgtag agcacgcagg
     1801 aataaggatc gaagcccggc gcgaacggaa ccgcggcctt gtagatgtcc gggtgttcgg
     1861 ccaggcagcg gaacgacgag taggcgcccc agctgccgcc catgatgccg acgcggccca
     1921 ggtcgatgta cgaatatttc gcagcgaggt tgcgcaaagc cgcggcatgg tccgccgcca
     1981 gcgttccgca atagctgcca tagcaggcgt cgtgaaaagc cttgctgcgc tccggcgtgc
     2041 cgcacgcgtc gagcatcacc accacgtaac cgtactgcgc cacgcgcagg gccatgccgc
     2101 ccatcgacag ggcaacggcg gcaaagccat ggtcggcgac cgccatctgc ggaccgccgt
     2161 agatgtattc gatcaccggg tatttctttt tcggatcgaa gtcgtgcggt ttgtagatca
     2221 cgccccacag gtcggtgctg ccatcggcag ccttcacgca gaactcttcg ggctcggcga
     2281 agccgacctg ctgcagccgg gaaatatctg ccttcgacac ctcggcgttc aacagcgtgc
     2341 cgtcgatgcg gcgcaggctg gtgaccggcg gggtcgaggg cgtggagcgc gtgtcgagga
     2401 atacctggcc gtgcggcgcg atctggcagg cgtgcttgcc cttgccctcg gtcagcacct
     2461 tcacggcgcc gccgccgagc ggaacacggc acaggtgcac gtcatacggg cgcgctgtat
     2521 cggtgtgcgc ggtgaagtag acatcgttgc cgatcacccg cgtcacgtcg cacaccgccc
     2581 agtcgccctg ggtcagttgc gccaccggct tgccgttcag gtcgtactgg tagatgtgct
     2641 tccagccatc gcgctccgac agccacagca gatgcctgcc gtcgggcgtc agcgtcaggc
     2701 cggtcttgcg gccgtagtag atgtcgtgat ggatgcggac gaaagtggca ccctcctcgg
     2761 tgaacagcag gcgcgtggcg ccgctgaccg cgttggccgc gtagatctcc acgcgccggc
     2821 agtcacggct catcacgaac accagcagtt ccgagccatc cggcagccag ccggcaaagc
     2881 acgggtactt gtcgcgcgtg tcgcccagct ggatttcgac cggcgcgcgg ccagccacgt
     2941 ccagcacata caggctcagc ttctccagca cgccgccagc cttgcagaaa ttgcggtaga
     3001 cgacctcgtc gttgcgcttg aggtaatgca cctgcggcgc ctgcacgacg ccgcggtagt
     3061 cgacacggta agcagcgatg cggttgccct gtggcgacca gttggtggcg ggcgcgccca
     3121 ggcccatcat cacgtacatc gggttggtcc agtcgatgtt ccactccact gcgggaatgc
     3181 cgtcatgggt gagttgcgtg gagcggccgt cgacggtcgc gcgcagggcg acgttgtggt
     3241 tctggatcga cagcatccag ttgccatcgg gcgacggcac ttcgtaagcg tcggtcggat
     3301 cgaccagcgg cgtgctgcgc ttgaacggcc gcggcgtggt gcgcgcttca accgtcatgc
     3361 cgaggtaggt gtcgagggcg ttgggggccg gcgccttgaa ggcggagccg ctggc
//
LOCUS       DASZZC010000718         6792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_691975,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000718 DASZZC010000000
VERSION     DASZZC010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691975"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..587
                     /locus_tag="VHE37_15215"
     CDS             117..587
                     /locus_tag="VHE37_15215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HVT36938.1"
                     /translation="MNINLSLIAEGIVFAIFIWFSAKMIWPPLMVAIEQRQKTIADGL
                     AAAERGSKALEDASAKSDEALKAARVQAQEILASASKQASQIVEAAKSQAQTEADRIK
                     QSARGEVERETSKAREELRQKVGELAVAGASKILKREIDAKAHADVLKDLAARV"
     gene            587..1123
                     /locus_tag="VHE37_15220"
     CDS             587..1123
                     /locus_tag="VHE37_15220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HVT36939.1"
                     /translation="MADLSTIARPYAKAVFELARESGKLKEWSAQLAAIAAVIADKQV
                     AELVGSPALSRAQLAALLGELLKRDVNSEGQSLLRMLAENGRLKAAPLIAGQYEALRA
                     EAELRVDVEITSAAPVAAEQQQTLASAIKKRLARDVAVEWKTDDSLIGGAVIRAGDLV
                     IDGSVRGELERLQTALAR"
     gene            1160..2659
                     /gene="atpA"
                     /locus_tag="VHE37_15225"
     CDS             1160..2659
                     /gene="atpA"
                     /locus_tag="VHE37_15225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HVT36940.1"
                     /translation="MQLNPSEISDLIKSRIKNFESVTEARNTGTIVSLQDGIVRVHGL
                     SDALQGEMLEFSGNTFGLALNLERDSVGAVVLGDYKHLAEGQTVKTTGRILEVPVGPG
                     LLGRVVNALGQPIDGKGAITAESYSPIEKVAPGVIARQSVSQPVQTGYKSVDAMVPIG
                     RGQRELIIGDRQTGKTAMAIDTIINQKKSGIKCVYVAIGQKASSIANVVRKLEENGAM
                     ANTIVVAASASESAALQFIAPYSGCSMGEYFRDKGEDALIIYDDLSKQAVAYRQVSLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNADYVEKFTNGRVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDMFNAGIRPAMNAGISVSRVGGAAQTKVIKKLAGG
                     TRTNLAQYRELAAFAQFASDLDEATRKQLERGQRVTELMKQKQYAPLSVGQMAASLYA
                     VEKGYIDRVEVSKVLAWEEGLHQYLANSAKATLDKLDGGDWNDGLEAEL"
     gene            2719..3579
                     /gene="atpG"
                     /locus_tag="VHE37_15230"
     CDS             2719..3579
                     /gene="atpG"
                     /locus_tag="VHE37_15230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HVT36941.1"
                     /translation="MPGAKEIRTKIKSVKNTQKITRAMEKVAMSKMRKAQERMTLARP
                     YAQLIRRTLGHLANANLEYKHPFTVERQVKRVAFLIVSSDRGLAGGLNMNVFRATVRS
                     IREWKDKGVEVDVALVGGKAISFFKRVGGKVLATASQLGDKPKIDQLLGTIKVLSDAY
                     RDGQVDRVFLVSNSFVNTMTQRPTVAQLLPIEPVKDENLLEHWDYLYEPDAKELLDSV
                     LQRYVESQVYQAVIENAACEQSARMVAMKSASDNAGKIISKLQISYNKARQANITKEL
                     SEIVGGAAAV"
     gene            3602..4999
                     /gene="atpD"
                     /locus_tag="VHE37_15235"
     CDS             3602..4999
                     /gene="atpD"
                     /locus_tag="VHE37_15235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HVT36942.1"
                     /translation="MSTGKIVQIIGAVVDVEFPRDAIPKVYDALKIEGSPLTLEVQQQ
                     LGDGTVRCIALGSSEGLKRGLVCNNTSKPISVPVGPATLGRIMNVLGEPIDEEGPVNA
                     KEHWSIHRAAPTYEDQAGSTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMMESKVIVEGNLSESKVAMVYGQMNEP
                     PGNRLRVALTGLTMAEYFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPT
                     LALEMGELQERITSTKKGSITSIQAVYVPADDLTDPSPATTFAHLDSTVVLSRDIAAL
                     GIYPAVDPLDSTSRQLDPNVIGAEHYNTARDVQGVLQRYKELKDIIAILGMDELSEAD
                     KQVVARARKIQRFLSQPFHVAEIFTGSPGKYVSLKETIRGFRAIVDGQMDHLPEQAFY
                     MVGTIDEAVEKAKKL"
     gene            5009..5425
                     /locus_tag="VHE37_15240"
     CDS             5009..5425
                     /locus_tag="VHE37_15240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HVT36943.1"
                     /translation="MANLHVDIVAAEGHIYSGEATMVFAPASQGEVGIAPRHAPLLTT
                     LKPGTVRVQNGSEEHAFFVAGGILEIQPHLVSVLADTALRSKDIDEAAAAKAKADAEA
                     KLAGAKTEMDLKHAQQELIEAAARLEFVKKIQKTSR"
     gene            complement(5422..6324)
                     /locus_tag="VHE37_15245"
     CDS             complement(5422..6324)
                     /locus_tag="VHE37_15245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT36944.1"
                     /translation="MNTPAVSVILPTYNRAHTLRRAIDSVLAQTHADLELIVVDDGST
                     DDTAALLASIADPRLRVERPPANEGAAAARNRGISAARGEWLAFQDSDDQWLAQKLER
                     QMALAAADIGLVLCGYQVRDGTRSIHVMPRSTLGGGPAQVDALDGWPIITPTWLVRRE
                     LLRELGGFRTDYRVFEDWDLAFRIIERCRVAAVGEPLLIKFGSSDALCANPRTLRDGL
                     AQLLADHASRWAAEPQRLARRYAHLGALQFVSGQRQQSRASLARAARLAPASPALPMW
                     MAAQLGRGVGWLAQRYPRFASMQL"
     gene            complement(6321..>6792)
                     /locus_tag="VHE37_15250"
     CDS             complement(6321..>6792)
                     /locus_tag="VHE37_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT36945.1"
                     /translation="REGTAEKLAEFARTHPHLHYPDQDALNVVCRGRWLHLQPRWNAQ
                     STFFQLRDTDLPLPPEQSREARAHPALVHYIGPFKPWTYMCTHPLRQLYFDHARQTPW
                     GAPAPEGRTLRTMLLRPLPLRWIDRWLAFERWLQALPQRVVRRLRRAGLPLPSA"
BASE COUNT         1281 a         2249 c         2252 g         1010 t
ORIGIN      
        1 tgatcgacgc ctcgttcatc atcggcgtgg gccttgcgat gatgttcgcc ttcgccaacc
       61 cgctgctgtc ggtggttcac taaaaacccc ggcaacgcgt cgcaagaggt gcgggcatga
      121 atatcaacct gagcctgatc gccgaaggca tcgtcttcgc gatcttcatc tggttctccg
      181 ccaagatgat ctggccgccc ttgatggtgg cgatcgagca gcggcagaag accatcgccg
      241 atggcctcgc cgccgccgag cgcggcagca aggcgctgga agatgcgtcg gccaagagcg
      301 atgaagcgct gaaggccgca cgcgtgcagg cccaggagat cctggccagc gccagcaagc
      361 aggcgtcgca gatcgtcgag gcggcgaaga gccaggcgca gaccgaggcc gatcgcatca
      421 agcagtcggc ccgtggtgaa gtcgagcgcg agaccagcaa ggcgcgcgag gaactgcgcc
      481 agaaggttgg cgagctggcc gtggccggcg cgtcgaagat cctcaagcgc gagatcgacg
      541 ccaaggccca cgccgacgtg ctcaaggatc tggcggcgcg cgtgtaatgg ccgatctctc
      601 gaccatagcg cggccctacg ccaaggcggt gttcgagctg gcgcgcgagt ccggcaagct
      661 caaggaatgg tcggcgcagc tcgccgccat tgccgcggtc atcgccgaca agcaggtggc
      721 cgaactggtc ggcagcccgg cactgtcgcg cgcgcagctg gcggcgctgc tgggcgaact
      781 gctcaagcgc gacgtcaaca gcgaaggcca gagcctgctg cgcatgctgg ccgagaacgg
      841 ccggctcaag gccgcgcccc tgatcgccgg gcaatacgaa gcgctgcgcg ccgaggctga
      901 attgcgggtg gacgtggaaa tcacctcggc ggcaccggtg gcggcggaac agcagcagac
      961 gctggccagc gcgatcaaga agcgcctggc gcgcgacgtg gcagtggaat ggaagaccga
     1021 cgattccctg atcggcggcg cggtgatccg cgccggcgac ctggtgatcg acggctcggt
     1081 gcgcggcgaa ctcgagcggt tgcagaccgc gctggcgcgc taagcggtac tcaagaatca
     1141 cgaatcggaa ctgacagtca tgcagctcaa tccttctgaa atcagcgacc tcatcaagag
     1201 ccggatcaag aattttgaat ccgtcaccga ggcgcgcaac accggcacca tcgtgtcgct
     1261 gcaggacggc atcgtgcgcg tgcacggcct gtccgatgcg ctgcagggcg aaatgctgga
     1321 attctccggc aataccttcg gcctcgcgct gaaccttgag cgcgactcgg tcggcgccgt
     1381 ggtgctgggc gactacaagc acttggccga aggccagacg gtgaagacca ccggccgcat
     1441 cctcgaagtg ccggtcggcc ccggcctgct cggccgcgtg gtcaacgcgc tgggccagcc
     1501 gatcgacggc aagggcgcca tcactgcgga aagctacagc ccgatcgaga aggtcgcgcc
     1561 cggcgtcatc gcgcgccagt cggtcagcca gccggtgcag accgggtaca agtcggtcga
     1621 cgcgatggtg ccgatcggcc gcggccagcg cgagctgatc atcggcgacc gccagaccgg
     1681 caagaccgcg atggccatcg acaccatcat caaccaaaag aagtccggca ttaagtgcgt
     1741 gtacgtggcg atcggccaga aggccagctc catcgccaac gtggtgcgca agctcgaaga
     1801 aaacggcgcg atggccaaca ccatcgtcgt tgccgcctcg gcgtccgaat ccgccgccct
     1861 gcagttcatc gcgccgtact ccggctgctc gatgggcgag tacttccgcg acaagggcga
     1921 agacgccctg atcatctacg acgatctttc caagcaggcc gtggcctacc gccaggtgtc
     1981 gctgttgctg cgccgcccgc cgggccgcga agccttcccc ggcgacgtgt tctatctcca
     2041 cagccgcctg ctcgagcgcg ccgcgcgcgt caacgccgac tacgtcgaga agttcaccaa
     2101 cggccgcgtc aagggcaaga ccggttcgct cactgcgctg ccgatcatcg agacgcaggc
     2161 cggcgacgtg tccgccttcg tgccgaccaa cgtcatctcc atcaccgacg gccagatctt
     2221 cctggaaacc gacatgttca atgccggtat ccgtccggcc atgaacgccg gcatctcggt
     2281 gtcgcgcgtc ggtggcgcgg cccagaccaa ggtcatcaag aagctggccg gcggcacgcg
     2341 taccaacctc gctcagtacc gcgagctggc ggcgttcgcg cagttcgcct cggacctcga
     2401 cgaagcgacc cgcaagcagc tggagcgcgg ccagcgcgtc accgagctga tgaagcagaa
     2461 gcagtacgcc ccgctgtcgg tcggccagat ggccgcctcg ttgtacgcgg tggaaaaggg
     2521 ctacatcgac cgcgtggaag tctccaaggt tctcgcctgg gaagaaggcc tgcaccagta
     2581 cctggccaac agtgccaagg ccacgctcga caagctcgac ggcggcgact ggaacgacgg
     2641 cctggaagcc gagctgtagg cggcgttgga agcctattcc aagcagtcgg ccatctgatt
     2701 tcctgacagg acactcccgt gccgggcgca aaagagatac gcaccaagat caagagcgtg
     2761 aagaacacgc agaagatcac gcgcgcgatg gaaaaggtgg cgatgagcaa gatgcgcaag
     2821 gcgcaggagc gcatgacgct cgcgcgcccg tacgcgcagc tgatccgccg cacgctcggg
     2881 catctggcca acgccaacct cgagtacaag catccgttca ccgtggagcg ccaggtcaag
     2941 cgcgtggcct tcctcatcgt gtcgagcgat cgcggcctgg ccggcggcct caacatgaac
     3001 gtgttccgcg ccaccgtccg cagcatccgc gagtggaagg acaagggcgt cgaggtcgat
     3061 gtcgcgctgg tgggcggcaa ggccatctcg ttcttcaagc gcgtcggggg caaggtgctg
     3121 gccacggcct cgcagctcgg cgacaagccg aagatcgacc agttgctcgg caccatcaag
     3181 gtgctgtccg acgcctaccg cgacggccag gtcgaccgcg tgttcctggt gtccaacagc
     3241 ttcgtcaaca ccatgacgca gcggccgacg gtggcgcagc tgctgccgat cgagccggtc
     3301 aaggatgaaa acctgctgga gcactgggac tacctgtacg agccggatgc caaggaactg
     3361 ctcgacagcg tgctgcagcg ctacgtcgag tcgcaggtct accaggcggt gatcgagaac
     3421 gccgcgtgcg agcagtccgc gcgcatggtg gcgatgaagt cggcatcgga caacgccggc
     3481 aagatcatca gcaagctgca gatctcgtac aacaaggcgc gtcaggccaa catcaccaag
     3541 gagctgtccg agatcgtcgg cggcgccgca gcggtttaaa gaattctcca gggttgaatc
     3601 catgagcaca ggaaagattg ttcagatcat cggcgccgtc gttgacgtgg agttcccgcg
     3661 cgacgcgatc cccaaggtct acgacgcgct gaagatcgaa ggctcgccgc tgacgctgga
     3721 agtgcagcag cagctgggcg acggtaccgt gcgctgcatc gcgctgggct cgtcggaagg
     3781 cctcaagcgt gggctggtgt gcaacaacac cagcaagccg atctccgtgc cggtcggtcc
     3841 ggccacgctc ggccgcatca tgaacgtgct cggcgagccg atcgacgagg aaggcccggt
     3901 caatgccaag gagcattggt cgatccaccg cgcggcgccc acctacgaag accaggccgg
     3961 ttcgaccgag ctgctggaaa ccggcatcaa ggtcatcgac ctgctgtgcc cgtttgccaa
     4021 gggcggcaag gtcggcctgt tcggcggcgc cggcgtgggc aagaccgtca acatgatgga
     4081 actgatcaac aacatcgcca aggcgcactc gggcctgtcg gtgttcgccg gcgtgggcga
     4141 gcgtacccgc gaaggcaacg acttctacca cgagatgatg gaatccaagg tcatcgtcga
     4201 gggcaacctc agcgagtcca aggtggcgat ggtgtacggc cagatgaacg agccgcccgg
     4261 caaccgcctg cgcgtggcgc tgaccggtct caccatggcc gaatacttcc gcgacgaggg
     4321 ccgcgacatc ctgttcttcg tcgacaacat ctaccgctac acgctggccg gcaccgaagt
     4381 gtccgcgctg ctcggccgca tgccgtcggc ggtgggttac cagccgacgc tggccctgga
     4441 aatgggcgag ctgcaggagc gcatcacctc gaccaagaag ggctcgatca cctcgatcca
     4501 ggccgtgtac gtgcccgcgg acgacctgac cgatccttcg ccggcgacca ccttcgcgca
     4561 cctggactcc accgtggtgc tgtcgcgtga catcgccgcg ctgggcatct acccggccgt
     4621 cgacccgctg gattccacca gccgccagct cgacccgaac gtcatcggcg cggagcatta
     4681 caacaccgcg cgcgacgtgc agggcgtgct gcagcgctac aaggaactca aggacatcat
     4741 cgccatcctg ggcatggacg aactgtccga agccgacaaa caggtagtgg cgcgcgcgcg
     4801 caagatccag cgcttcctga gccagccgtt ccacgtcgcc gaaatcttca ccggctcgcc
     4861 cggcaagtat gtctcgctca aggaaaccat ccgcggtttc cgtgcgatcg tcgacggcca
     4921 gatggaccac ctgcccgagc aggcgttcta catggtcggc accatcgacg aagcggtcga
     4981 gaaggccaag aagctctaag agatcgtcat ggcaaacctg cacgtcgata ttgtcgccgc
     5041 cgaaggccac atctacagtg gcgaggcgac gatggtgttc gcgccggcat cgcagggcga
     5101 ggtcggcatc gcgccgcgcc acgcgccgct gctgaccacg ctcaagcccg gcaccgtgcg
     5161 cgtgcagaac ggcagcgaag aacatgcgtt cttcgtcgcc ggcggcatcc tggaaatcca
     5221 gccgcacctg gtttcggtgc tggctgacac cgcgctgcgc agcaaggaca tcgatgaagc
     5281 cgcggccgcc aaggccaagg ccgacgccga ggccaagctg gccggcgcca agaccgagat
     5341 ggacctgaag cacgcgcagc aggaattgat cgaagccgcc gcgcggctcg agttcgtcaa
     5401 gaagatccag aagaccagcc gctagagctg catgctggca aagcgcggat agcgctgcgc
     5461 cagccagccc acgccgcgcc cgagttgcgc ggccatccac atcggcaagg ccggcgacgc
     5521 cggcgccagg cgtgctgccc gcgccaggct cgcgcgtgac tgttgccgtt gcccgctgac
     5581 gaactgcagg gcgcccaggt gcgcgtagcg ccgcgccagg cgttgcggtt ctgccgccca
     5641 gcgcgacgcg tgatcagcca gcagctgtgc tagcccgtcg cgcagcgtgc gcgggttggc
     5701 gcacagggcg tcgctgctgc cgaacttgat cagcagcggc tcgccaacgg cagccacgcg
     5761 gcaacgctcg atgatgcgga atgcgagatc ccagtcctcg aacacgcgat agtcggtgcg
     5821 gaagcctccg agttcgcgca gcagttcgcg tcgcaccagc caggttggcg tgatgatcgg
     5881 ccagccgtcc agcgcatcca cctgggcagg tccaccgccg agcgtgctgc gtggcatcac
     5941 gtggatgctg cgcgtgccgt cgcgcacttg gtaaccgcac agcaccagtc cgatgtcggc
     6001 ggcggcgagg gccatctgcc gctccagctt ctgcgccagc cactggtcgt cactgtcctg
     6061 gaacgccagc cattcgccgc gcgcggcgct gatgccgcgg ttgcgcgccg ctgccgcgcc
     6121 ttcgttcgcc ggcggccgtt cgacgcgcag gcgcggatcg gcaatggacg ccagcagcgc
     6181 ggcggtgtcg tcagtcgagc cgtcatcaac cacgatcaat tccaggtcgg cgtgcgtttg
     6241 cgccagcacg ctgtcgatgg cgcgtcgcag cgtgtgcgcg cggttatagg tgggcaggat
     6301 gacgctaacc gccggcgtgt tcatgcgctg ggcagcggca aaccggcgcg gcgcagacgc
     6361 cgcaccacgc gctgcggcag cgcctgaagc cagcgctcaa acgcgagcca gcggtcgatc
     6421 cagcgcagcg gcagcgggcg cagcagcatg gtgcgcagcg tgcggccttc cggcgccggc
     6481 gcgccccatg gcgtctgccg tgcgtggtcg aaatagagtt gcctcaacgg gtgcgtgcac
     6541 atataggtcc acggcttgaa cgggccgatg taatgcacca gcgccggatg tgcgcgtgcc
     6601 tcccgcgatt gctccggcgg cagcggcaga tcggtgtcgc gcagctggaa gaaagtcgac
     6661 tgcgcattcc agcgcggctg caggtgcagc cagcgcccgc ggcagaccac gttgagcgcg
     6721 tcctggtcgg ggtagtgcag gtgtggatgc gtgcgcgcga attccgccag cttttccgcc
     6781 gtgccctcgc gg
//
LOCUS       DASZZC010000719         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_382399,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000719 DASZZC010000000
VERSION     DASZZC010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382399"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VHE37_15255"
     CDS             <1..798
                     /locus_tag="VHE37_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36946.1"
                     /translation="SNGDGNLSGLDDSSFMSGYWSNVTLLFQQLASYNKPALVNLEPD
                     FWGYAQQQAPGGDPAQLAARVSINSDCAGLPDNVVGIAQCLMRSARKYAPRAYVGFPV
                     SEWGANASADVVAFMNRIGADQADFIVITTLDRDAGCFEVRPQPASCSRSGSGWYWDE
                     SNSTHPNFHDHLAAATSYHQGIGGLPLLWWQTPLGVPSSVPGGASGHYRDNRMHYILN
                     HATEFVAAGGVGVVFSAGAGGQTDITTDGGQFQNLSGQYLAAPAALP"
     gene            complement(795..1721)
                     /locus_tag="VHE37_15260"
     CDS             complement(795..1721)
                     /locus_tag="VHE37_15260"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVT36947.1"
                     /translation="MAQWSAADIPDLSGRNAIVTGANGGLGYYTALELARAGARVTLA
                     CRSQRKGEEAMEHIRALVPGADIELMSIDMGDLASVRKFAGAWKRKKRKLHLLVNNAG
                     IVAQPLSRTPQGFELQMGVNYFGPFALTGLLLPLIKSTPGARIVNVASVAHKFGWLPL
                     DDVNWERRRYNEWLGYGQSKLAMLLWTYELQRRLKKSGARAIALAAHPGYAATNLVSS
                     EMRLARHWLGRKALDLGNKLFAHSAHDGALCSLYAATAAAAKGGDYIGPGGLGEMRGP
                     PKKVKSTRASHDAAAARKLWTLSEQLTGVDYL"
     gene            complement(1898..>3213)
                     /locus_tag="VHE37_15265"
     CDS             complement(1898..>3213)
                     /locus_tag="VHE37_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT36948.1"
                     /translation="TRLRRYVGMEPGSTPLTQLARAQMQEKFGDKKLQGPYRGQVEQD
                     LADSASLVDGVRQLYAQFGITGQDDALAALDQQIKEQDDWVRANILPRASADHRLPPD
                     IYADNLKNFGTKVEPHELIRRALLAFIEIQNEMQSIATRIAREKGWKSGDYRDVIREL
                     KKQQVSGKAILPLYADTLAKIEDIIRREHIVTLPARKAAIELSSPAEAALLPAPHMNP
                     PRLVGNTGEYGIFVLPLTVPTGPGEKALKTDDFTFKAAAWTLTAHEARPGHELQFASM
                     VERGVSQARAIFAFNSVNVEGWGLYSEAEIKPYMPLDGQLIGLQHRLMRAARAYLDPM
                     LNLGLMQPEDAARVLSHDVVLSDGMVKQEVDRYTFRAPGQATSYFVGYQALMETRERA
                     QLALKDKFDRQRFNDYVLGQGLLPPELLQKAVMEDFVPTELKRGS"
BASE COUNT          486 a         1061 c         1090 g          576 t
ORIGIN      
        1 agcaacggcg acggcaatct ctccggcctc gacgacagca gcttcatgag cggctactgg
       61 agcaacgtca cgctgctgtt ccagcagctg gcctcgtaca acaagccggc gctggtcaat
      121 ctcgagcccg acttctgggg ctacgcgcag cagcaggcgc ctggcggcga ccctgcgcag
      181 ctggcggcgc gcgtcagcat caacagcgac tgcgccgggc tgcccgacaa tgtcgtcggc
      241 atcgcgcaat gcctgatgcg cagcgcacgc aagtacgcgc ccagggcgta cgtgggattc
      301 ccggtgtctg agtggggcgc gaatgccagc gcggatgtgg tcgccttcat gaaccgcatc
      361 ggcgcggacc aggcggattt catcgtcatc accacgctgg accgcgacgc cggctgcttc
      421 gaggtgcgcc cgcagccggc atcgtgctcg cgctcgggca gcggctggta ctgggacgag
      481 agcaacagca cgcatcccaa cttccacgat cacctggcgg cggcgacgtc ctatcaccag
      541 ggcatcggcg gattgccgct gctgtggtgg cagacgccgc tgggcgtgcc gtcgtcggtc
      601 cccggcggcg ccagcgggca ttaccgcgac aatcgtatgc actacatcct caatcatgct
      661 acggaattcg tagcggcggg cggggtgggc gtggtgttca gcgccggcgc cggcgggcag
      721 accgacatca ccaccgatgg cgggcagttc cagaacctgt ccggccagta cctggccgcg
      781 ccggccgcat tgccctagag ataatccacg ccggtgagct gctccgacag cgtccacagc
      841 ttgcgcgcgg ccgcggcatc gtgcgaggcg cgggtagact tcaccttctt cggcgggccg
      901 cgcatttcgc cgagtccgcc gggcccaatg tagtcgccac ccttggctgc agccgcggtg
      961 gcggcgtaga gcgagcacag cgcgccgtca tgcgccgagt gcgcgaacag cttgttgccc
     1021 aggtccagcg ccttgcggcc gagccagtgg cgtgccagcc gcatctcgct ggacacgagg
     1081 ttggtggcgg cgtagcccgg gtgcgcggcc agcgcgatgg cgcgcgcccc ggatttcttc
     1141 agccgccgtt gcagctcgta ggtccacagc agcatcgcca gcttgctctg gccgtagccg
     1201 agccactcgt tgtagcggcg gcgctcccag ttgacgtcgt ccagcggcag ccagccgaac
     1261 ttgtgggcga cgctggccac gttgacgatg cgcgcgccgg gtgtggactt gatcagcggc
     1321 agcagcaggc cggtgagcgc aaacgggccg aagtagttca cgcccatctg cagttcgaaa
     1381 ccctgtggcg tgcgcgacag cggctgcgcc acgatgccgg cgttgttcac cagcaggtgc
     1441 agcttgcgtt tcttgcgctt ccaggcgccg gcgaacttgc gcaccgatgc gagatcgccc
     1501 atgtcgatgc tcatcagttc gatgtcggcc ccgggcacca gggcgcggat gtgctccatc
     1561 gcttcctcgc ccttgcgctg cgagcggcag gccagcgtca cgcgcgcgcc ggcacgcgcg
     1621 agttccagcg cggtgtagta gccgagcccg ccgttggcgc cggtgacgat ggcgttcctg
     1681 ccggaaagat cgggaatgtc tgctgcggac cactgcgcca tggttgtgct ccctggatgg
     1741 cgcgccagac tacccgggcg cggccacggc gcccaactgc gttcggacga gcttcaccgc
     1801 accccgtttc gcgctgttat aaaaaatcga gttaaagtaa aaacaatcga tattaagtaa
     1861 cacgcaaaac cggggtgcgg tgagcggatt ttttcgatca gctgccgcgc ttcagctcgg
     1921 tgggcacgaa gtcttccatc accgctttct gcagcagttc cggcggcagc aggccctggc
     1981 ccagcacgta gtcgttgaag cgctgacgat cgaacttgtc cttcagcgca agctgcgcgc
     2041 gctcgcgcgt ttccatcagc gcctggtagc cgacgaaata ggaggtggcc tgcccgggcg
     2101 cccggaaggt gtagcggtcg acttcctgct tgaccatgcc gtcagacagc accacgtcgt
     2161 gcgacagcac gcgcgcggcg tcctccggct gcatcagccc gaggttgagc atcgggtcca
     2221 ggtacgcgcg cgcggcgcgc atcagccggt gctgcaggcc gatcagctgt ccgtcgagcg
     2281 gcatgtacgg tttgatctcc gcttcggaat agaggcccca gccttcgacg ttgacgctgt
     2341 tgaaggcgaa gatggcgcgc gcctgcgaca cgccgcgctc gaccattgag gcgaactgca
     2401 gctcgtggcc gggcctcgct tcgtgcgcgg tgagcgtcca ggccgccgcc ttgaaggtga
     2461 agtcgtcggt cttcagcgct ttctcgccgg gcccggtcgg caccgtcagc ggcagcacga
     2521 agatgccgta ttcgccggtg ttgcccacca gccgcggcgg gttcatgtgc ggtgcgggca
     2581 ggagggcagc ctcggccggg ctcgacagtt cgattgccgc cttgcgcgcg ggcagggtga
     2641 cgatgtgctc gcggcggatg atgtcctcga tcttcgccag cgtgtcggcg tacagcggca
     2701 ggatggcctt gccactcacc tgctgctttt tcagctcgcg gatcacgtcg cggtagtcgc
     2761 cgctcttcca gcccttttcc ctggcgatgc gcgtggcgat ggactgcatc tcgttctgga
     2821 tttcgatgaa cgccagcagc gcgcggcgga tcagctcatg cggctcgacc ttggtgccga
     2881 agttcttcag gttgtcggca tagatgtcgg gcggcagacg gtggtcggca ctcgcgcgcg
     2941 gcaggatgtt tgcgcgcacc cagtcgtcct gttccttgat ctgctggtcg agcgcggcca
     3001 gggcgtcgtc ctggccggtg atgccgaact gcgcgtagag ctggcgcacg ccgtcgacca
     3061 ggctggccga gtcggccagg tcctgttcca cctggccgcg gtatggaccc tgcagcttct
     3121 tgtcgccgaa tttttcctgc atctgcgcac gcgccagctg tgtgagcggc gtgctgcccg
     3181 gctccatgcc cacgtagcgg cgcaggcggg tga
//
LOCUS       DASZZC010000720         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1213711,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000720 DASZZC010000000
VERSION     DASZZC010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213711"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..229
                     /locus_tag="VHE37_15270"
     CDS             56..229
                     /locus_tag="VHE37_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36949.1"
                     /translation="METLHVLPSWRGVIRIVHERRLQHGGESKEGEEGGSQEKGCKED
                     CKEEEVVLGLTAF"
     gene            415..1269
                     /locus_tag="VHE37_15275"
     CDS             415..1269
                     /locus_tag="VHE37_15275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HVT36950.1"
                     /translation="MTGYARAEAQGPWGRISWELRSVNHRYLDLQIKVPEEFRRIENE
                     LRTQAGARVARGKIEAGLRYVREASADGALEVDLARLKQLKSAMETVSRELHSTGLPD
                     PLRVLGFPGVVRQQEADFAPLIAAARELFEQALDDFTATRTREGERLAAYLAERTQAI
                     EQLVAQVRARYPQVRDGWLARVKARCAELGVEVDPQRLAQEVALSAQRLDVEEEMSRL
                     ASHVAEVRQTLSRGEAIGRRLDFLMQELNREANTLSSKSQDAEMTRCAVEMKVIIEQM
                     REQVQNIE"
     gene            1514..2527
                     /locus_tag="VHE37_15280"
     CDS             1514..2527
                     /locus_tag="VHE37_15280"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT36951.1"
                     /translation="MTTHTAEMLPETVDASDRDASAPLDAQQRALLELGRRLRASGYR
                     HVTVTPETHRRVLARTGPARAGLRDALGWSRPFARDAIPADALALLERAGAIEHCGAQ
                     LRSRVRWSTLDDLLLVHSAYPTLAADAVFFGPDTYRFGELVQRALTTRHHRVRHLVDV
                     GCGSGAGGLLAAHMLDVPAEVTLADINPQALRLAAVNAALNGVPHARALRSDLLGAVQ
                     PPIDLVLCNPPYLCDPSQRAYRHGGGALGCALALRVVSEALARLAPGGRLVLYTGSPM
                     VDGQDTFFDAARPLLQAAGAQWQYREIDPDVFGEELDAPAYARVERIAAVGLIAQMPG
                     GDA"
     gene            2524..3495
                     /locus_tag="VHE37_15285"
     CDS             2524..3495
                     /locus_tag="VHE37_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HVT36952.1"
                     /translation="MSADGTAATGYERDHRDLARHNQLRLRPGCIDDDWSSALCDDFE
                     MRMLEGRLVEEQRRALASLLEDVPDEADAFMHWFEQLRERGPGQRDALFPWLAQQADL
                     AAMRWFITQEMAGEAGFDDLVALTQVRMPVQAKLELARNYWDEMGRGHEDGMHGPMLA
                     ELGRALGLQPSIEGTVWESLALANLMVALAANRRYAYHSIGALGVIEMTAPGRVAQVN
                     EGLRRLGLGGRERRYFQLHAGLDLKHSADWNREVIRPLVEHDPALARPIAEGALLRLA
                     AGARCFERYRRELGVQPRASAPRYSSQPQLRNRAWSPPSSLLPMSLR"
BASE COUNT          598 a         1159 c         1237 g          561 t
ORIGIN      
        1 tgagtgacag cccaaagagt tgaccttttc tacatttata agtaaaaatt cacatatgga
       61 aacactgcat gtgcttccat cctggcgcgg agtgatccgc atcgttcatg aacggaggtt
      121 gcaacatggc ggcgaaagca aagaaggcga agaaggcggc tcccaagaaa aaggctgcaa
      181 agaagactgc aaagaagaag aagtagtctt aggcctcacg gccttttgaa cgggcggcat
      241 aaagccgccc gtctcgtttt tcaagaccgg aaagaagcag gcggcacgcg cgaaactttt
      301 cggcatcgat aagcaggcgc gcaatgtgcc tgaacagtga gtcggatggc ggcggcacgc
      361 ggcgattaca atgccgctcc agtttccgcc gatggtacct ccatgatacg cagcatgacc
      421 ggttacgccc gcgccgaggc gcaggggcca tggggacgca tcagctggga gttgcgctcg
      481 gtcaatcatc gttatctcga cctgcagatc aaggtgccgg aggaattccg tcgcatcgaa
      541 aatgaactgc gcacgcaggc cggcgccagg gtggcgcgcg gcaagatcga ggccgggctg
      601 cgctacgtgc gcgaggcctc ggccgacggc gcgctcgagg tcgacctggc gcggctcaag
      661 cagttgaagt cggcgatgga aacggtgagc cgcgaactgc attccaccgg cctgcccgat
      721 ccgctgcgcg tactcggctt tcccggcgtg gtgcgccagc aggaagcgga cttcgcgccg
      781 ctgatcgccg ccgcgcgcga actgttcgag caggcgctgg atgatttcac cgccacgcgc
      841 acgcgcgagg gcgagcgcct ggccgcgtac ctggcggagc gcacgcaggc catcgagcag
      901 ctggtggctc aggtgcgcgc gcgctatccg caggtgcgcg acggctggct ggcgcgcgtg
      961 aaagcgcgct gcgccgagct cggcgtggaa gtggatcctc agcggctggc gcaggaagtg
     1021 gcgctgtcgg cgcagcggct cgacgtggaa gaagagatgt cgcgcctggc cagccacgtg
     1081 gccgaagtgc ggcagacgct gtcgcgcggc gaggccatcg gccgccgtct ggatttcctc
     1141 atgcaggaac tcaaccgcga agccaacacg ctgtcatcca aatcgcagga cgcagagatg
     1201 acgcgttgtg cggtggagat gaaggtgatc atcgagcaga tgcgcgagca ggtgcagaac
     1261 atcgagtgag ccgaaccgcg acctctaccg cactgccttt tgcggtgttt taaattatat
     1321 aattaattaa acattgatcg atattagata acatcgcctg caggggtgcg gtggagtctt
     1381 ttccacgcgc gcacggctga accttggggc gcgccggtgt ttctaagctg cgaaggcgcc
     1441 ggcattgccg cgctccatga cccaataacg gatgcgcgtg ccgcactgtc gcgccatccc
     1501 ctgcgcagat tcgatgacca cacacaccgc cgagatgctg ccggaaacgg tcgacgcttc
     1561 cgaccgcgac gcgtcggcgc cactcgacgc gcagcagcgt gccctgctcg aactcgggcg
     1621 ccggctgcgt gcttcgggat accggcacgt cacggtgacg ccggagacgc accgccgtgt
     1681 gctggcgcgc acaggacctg cgcgggccgg cttgcgcgat gcgctgggct ggagccggcc
     1741 gttcgcgcgc gacgccattc ccgccgatgc gctggcgctg ctcgagcgtg ccggagccat
     1801 tgaacattgc ggcgcacagc tgcgcagccg cgtccgctgg tcgacgcttg acgatctgct
     1861 gctggtgcat tccgcttacc ccacgctggc cgccgacgcc gtttttttcg ggccggacac
     1921 ttatcgcttc ggcgagctgg tccagcgtgc gctgacgacg cgccatcacc gcgtgcgcca
     1981 cctggtcgac gtgggctgcg gcagcggtgc gggcggcctg cttgccgcgc acatgctgga
     2041 cgtgcctgcc gaagtaacgc tggccgacat caatccgcag gcgctgcgcc tcgccgcggt
     2101 caacgctgcg ctcaacggcg tgccgcacgc gcgcgccctg cgcagcgacc tgctcggcgc
     2161 agtgcagccg cccatcgacc tcgtgctgtg caacccgcct tacctgtgcg acccatcgca
     2221 gcgcgcatat cgccacggtg gcggcgcgct gggctgcgcg ctcgcgctgc gcgtggtgag
     2281 cgaagcgctg gcgcggctcg cgcccggcgg gcggctggtg ctgtacaccg gcagtccgat
     2341 ggtcgatggc caggacacgt tcttcgacgc ggcgcggccg ctgctgcagg cggcgggcgc
     2401 gcagtggcag taccgcgaga tcgaccccga cgtgttcggc gaggaactgg atgcgccggc
     2461 ctacgcgcgc gtcgagcgca tcgccgcggt cggcctgatc gcgcagatgc ccggaggcga
     2521 tgcatgagcg ccgacggcac cgcggcgact ggatacgagc gcgatcaccg cgacctggcg
     2581 cgccacaacc agctgcgcct gcggccaggc tgcatcgacg acgactggag cagcgcgctg
     2641 tgcgatgact tcgagatgcg catgcttgaa ggccgcctgg tggaggagca gcgccgcgcg
     2701 ctggcatcgc tgctggaaga cgtgcccgat gaagccgacg ccttcatgca ctggttcgag
     2761 cagctgcgcg aacgcggccc cggccagcgc gacgccctgt ttccatggct ggcgcagcag
     2821 gccgaccttg cagcgatgcg ctggttcatc acccaggaga tggccggcga ggccgggttc
     2881 gacgacctgg ttgcgctgac ccaggtgcgc atgccggtgc aggcgaagct ggaactggcg
     2941 cgcaattact gggacgagat gggccgcggc cacgaggacg gcatgcacgg cccgatgctg
     3001 gccgagctcg gccgtgcgct cggcctgcag ccttccattg aaggcacggt gtgggaatcg
     3061 ctggcgctgg ccaacctgat ggtggcgctg gccgccaacc gccgctacgc gtaccactcg
     3121 atcggcgcgc tcggcgtgat cgagatgacc gcgcccggcc gcgtggcgca ggtgaatgaa
     3181 ggactcaggc gcctgggcct gggcgggcgc gagcggcgct acttccagct gcacgccggg
     3241 ctggacctca agcactcggc cgactggaac cgcgaggtga tccgcccgct ggtcgagcac
     3301 gatcctgcgc tggcgcggcc gatcgctgaa ggcgcgctgc tgcggctggc cgcgggtgcg
     3361 cgctgcttcg agcgctatcg ccgcgagctc ggcgtgcaac cacgcgcgag cgcaccgcgt
     3421 tattcgagcc agccccagct gcgcaaccgc gcgtggtctc cgcccagcag cctgttgccg
     3481 atgtcgttgc ggtaacgcag gttgggcacg gccacttttc cggccaccgc gtaagcggcg
     3541 tcgcgcgcgg cgccc
//
LOCUS       DASZZC010000721         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_571102,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000721 DASZZC010000000
VERSION     DASZZC010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571102"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VHE37_15290"
     CDS             <1..953
                     /locus_tag="VHE37_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT36953.1"
                     /translation="TGETSMLRLLKWLLILAAVVFGGVLAYLHLDPVDATARAVALQR
                     QAGGLVRREITLPDGTLYVYLEGGQGRPLVLLHGFGANKDNWTRVARYLTPRYHVIAP
                     DHVGFGESAHPQDADYAPPAQAERLHQLAAALNLGKVDVGGSSMGGHIAMTWAAKYPD
                     EVQSLWLLDPGGVWSAPKSELATIIDAGGENPLMARTTDEYAAIFRFVMARPPFIPRP
                     ILDQFAKERIANYTLEQRIFKQIREDDVEDRVKGLATPTLIVFGDQDRAINPAAAGVL
                     QPLLPKSQVIIMKGLGHLPMIEDPKQSADDYLAFRAGIGG"
     gene            complement(920..>1679)
                     /locus_tag="VHE37_15295"
     CDS             complement(920..>1679)
                     /locus_tag="VHE37_15295"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HVT36954.1"
                     /translation="RAALRLLRPRLGERRYRAENLALRDAGRRLAPLRNAKALLEAYQ
                     DFCRRQAKQIAALPLGPLQQQLRAQLARTRRAQHGHADATGAAARDLRAVCLRMQGLR
                     LRSLPSLVPALHQLYRKCRRAMHRARKANTAQALHEWRKQVKYLAHALDALGDASGRH
                     LRRRAEKLADQLGDEHDLVLLSEALDQTTSALDAAVLAQLHALIDRRRARLRQSAFRL
                     GARAFRRKPRRYARKLRRRLAAESQPPMPARKAR"
BASE COUNT          246 a          602 c          596 g          235 t
ORIGIN      
        1 aaaccgggga gacatcgatg ctgcgcctgc tcaagtggct gctgatcctg gcggcggtgg
       61 tgtttggcgg agtgcttgcc tacctgcacc tggacccggt cgatgccacc gcccgcgccg
      121 tggcgctgca acggcaggcc ggcgggctgg tgcgccgcga gatcaccctg cccgacggca
      181 cgctctacgt ttacctcgag ggcggccagg gccggccgct ggtgctgctg cacggcttcg
      241 gcgccaacaa ggacaactgg acgcgcgtgg cgcgctacct cacgccgcgc taccacgtga
      301 tcgcgcccga ccatgtcggc ttcggcgaat cggcgcatcc gcaggacgcc gactacgcgc
      361 cgccggcgca ggccgagcgc ctgcaccagc tggccgccgc gctcaacctg ggcaaggtcg
      421 acgtcggcgg cagctccatg ggcgggcaca tcgccatgac ctgggcagcg aagtatcccg
      481 acgaggtgca gagcctgtgg ctgctcgatc cgggcggcgt gtggagcgcg cccaagagcg
      541 agctcgccac catcatcgac gctggcggag aaaatccgct gatggcgcgc accaccgacg
      601 agtacgccgc catcttcagg ttcgtcatgg ccaggccgcc gttcatcccc cgcccgatcc
      661 tcgaccagtt cgccaaggag cgcatcgcca actacacgct ggagcagcgc atcttcaagc
      721 agatccgcga agacgacgtg gaggaccgcg tgaaaggact ggccacgccc acgctgatcg
      781 tgttcggcga ccaggaccgc gccatcaacc cggcggcggc cggcgtgctg cagccgctgc
      841 tgccgaagtc gcaggtcatc atcatgaaag gcctgggcca cctgccgatg atcgaagacc
      901 cgaagcagtc ggcggacgat tacctggcct tccgcgccgg catcggcggc tgagattcag
      961 ctgccaggcg ccggcgcagc ttgcgcgcgt aacgccgcgg cttgcgccgg aaggcgcgcg
     1021 caccgagcct gaatgctgac tggcgcaggc gcgcgcggcg gcggtcgatg agcgcgtgca
     1081 gctgcgccag cacggcggcg tcgagcgcgc tcgtggtctg gtccagcgcc tcgctcagca
     1141 gcaccaggtc gtgctcgtcg cccagttgat cagccagttt ttccgcgcgc cggcgcaggt
     1201 gcctgccgga cgcgtcgccg agcgcatcga gcgcatgcgc caggtacttc acctgcttgc
     1261 gccattcgtg cagtgcttgc gcggtgttgg ccttgcgcgc gcggtgcatc gcgcggcggc
     1321 acttgcggta gagctggtgc agcgcgggca ccaaggacgg caggctgcgc aggcgcaggc
     1381 cctgcatgcg caggcacacg gcgcgcaggt cgcgcgccgc ggcgccggtg gcatccgcat
     1441 ggccgtgctg cgcgcgccgc gtacgcgcca gctgcgcgcg caactgctgc tgcagcggcc
     1501 ccagcggcag cgctgcgatc tgctttgcct ggcgccggca gaaatcctgg tacgcctcga
     1561 gcagcgcctt ggcattgcgc agcggcgcca ggcggcggcc ggcatcgcgc agcgccaggt
     1621 tctccgcgcg gtagcgccgc tcgcccaggc gcggccgcag caggcgcagc gccgcgcgg
//
LOCUS       DASZZC010000722         6213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_827063,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000722 DASZZC010000000
VERSION     DASZZC010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827063"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VHE37_15300"
     CDS             complement(<1..523)
                     /locus_tag="VHE37_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HVT36955.1"
                     /translation="MRIGIVVDSACDLPREFLEDNRIVILPITIHLGDADIVDKRNAE
                     ATLAFYQQHLGARGDASSSPLPVEHIKDLFLSKLVIDYDLVFCLTIASSRSPIYDNAT
                     KAALAILNDYKPIRAKAGVPGPFALRVIDCQNLFVAQGVVTVEAVRMVRDRENPNKIR
                     ERLEYLAQNTYAYM"
     gene            complement(580..954)
                     /locus_tag="VHE37_15305"
     CDS             complement(580..954)
                     /locus_tag="VHE37_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HVT36956.1"
                     /translation="MSGDPLVDALRVQLIAEREGFDWRHLDELWDKLAEEIGELKVAA
                     GEGRERAQDELGDLLFMAVNLARHLKVDANAALAGANAKFLRRWEHIMRHAHTLPPLG
                     APGRLEAMETLWQEAKRLEKGG"
     gene            complement(951..1583)
                     /locus_tag="VHE37_15310"
     CDS             complement(951..1583)
                     /locus_tag="VHE37_15310"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVT36957.1"
                     /translation="MKPADLEARLRRALHNTSEEPPRPISPIELPLNIDKLITPRLLN
                     SLRPAAVLVPVIRRARELTVLFTLRAETLRAHKGQISFPGGRRDEEDTSAAANALREA
                     QEETGLDPKLVEVVGYLDDYPTITRYRVTPVVGMVDGSASFTYDESEVAEVFEVPLSF
                     LLKVENFERKAFTRSGVNVPFFELNYQRWRIWGATAGMLWNLAQKVGQVK"
     gene            1732..2199
                     /locus_tag="VHE37_15315"
     CDS             1732..2199
                     /locus_tag="VHE37_15315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVT36958.1"
                     /translation="MSLTNIQGALRANFRRAVPHVRELGIEVSICEPEYVEMMLPYRE
                     DWLGDVERGVIHTGVITTLVDSACGLALLAHLSTLEPIATLDLRMDYLRPAFAPKPVW
                     CRAECYRSTENIVFMRASCWQDDRAQPVSATQAAFMRTTRSRPRPNMIPANEP"
     gene            2196..2636
                     /locus_tag="VHE37_15320"
     CDS             2196..2636
                     /locus_tag="VHE37_15320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVT36959.1"
                     /translation="MSRIRAAREAAQRGDHAALVDAIPYAKFLGVKLVTEGARLLSVL
                     PFRAGLVGNQTINAIHGGVTAAFMENAALLHLLLRLDQERVPKSIDFAIDYLLPGQAL
                     DTYAECKVSRAGVRVAQVQVNCWQKSEQVPIAVARAHFLLTAPE"
     gene            2633..2890
                     /locus_tag="VHE37_15325"
     CDS             2633..2890
                     /locus_tag="VHE37_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36960.1"
                     /translation="MSDYRGWITLREIDARLGQSKGTAFRRFKQLEPALREGADFVVL
                     DAQRDRAQIDALRAQQRIYASSINVILLSPSSAQRLAAAAQ"
     gene            2966..3280
                     /locus_tag="VHE37_15330"
     CDS             2966..3280
                     /locus_tag="VHE37_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HVT36961.1"
                     /translation="MSAATDLLKKLPAAFNPDGASDSTIQFNISTPMHIVIKGGACTL
                     NDGAAASPDVTITMEDDDFVALMKGELNGMTAFMTGKLQLDGDLMLAQRMGSMFDPAK
                     LG"
     gene            3273..3968
                     /locus_tag="VHE37_15335"
     CDS             3273..3968
                     /locus_tag="VHE37_15335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT36962.1"
                     /translation="MADRSKPQDQARGREASGFSFALEEKDGRLWLAALHRPEWTPIC
                     ADWLNAEMHSRVRAGRRQLLARALGLAKNPDQEILDATAGLGRDGWTLAALGARVTLC
                     ERTLQLYALQRDARARASAQAPEIAARVELLQADAHAVLAGARRWDAVYLDPMYPHRG
                     KSALPQKEMQLLRELTGGDDDADALLAPALACARRRVVVKRPPHAPHLDGRRPALSME
                     SAQARFDIYLAAV"
     gene            3976..4473
                     /locus_tag="VHE37_15340"
     CDS             3976..4473
                     /locus_tag="VHE37_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVT36963.1"
                     /translation="MRLLSLVRHAKSSWDYAELSDFERPLNERGRRDAPLMAKLCLIE
                     WGKPERLVSSPALRAITTARTFAEVFGIAGEEIIVQPKIYEAAVETLLRLVRALDDGD
                     RHVMLFGHNPGISALAHELARCHFDEMPTCAVAGFELAVKCWSDVGAGCGKLVRYSTP
                     KGSRD"
     gene            complement(4477..4995)
                     /locus_tag="VHE37_15345"
     CDS             complement(4477..4995)
                     /locus_tag="VHE37_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HVT36964.1"
                     /translation="MIYTIENRVPKFNGEVWVADNAVVIGSVTLEHESSVWFGCTVRG
                     DVDDIRIGEGTNVQDGSVLHTDVGIKLNIGKGCTIGHMVMLHGCDIGDNTLIGIKSLV
                     MNRARIGSNCIVGACSLVTEGKQFPDGSLIMGSPAKLVRPLTPQEIAMVALSGKHYVQ
                     NAKKFKAQLKPA"
     gene            5039..5635
                     /locus_tag="VHE37_15350"
     CDS             5039..5635
                     /locus_tag="VHE37_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HVT36965.1"
                     /translation="MMLVAAQLALSACASKPPPLPRLASDARLLAFGDSLTAGTGATA
                     EQAYPAVLGALIHREVINAGVPGETSSEGRVRLPQVLDDAQPQLVILCEGGNDMLRRM
                     DAAQMRANLTAMIREVQSRHIALVLLAVPHPAIFGLKADPVYASLADEFHLPVESSAL
                     SAIIGDNARKADQIHPNAQGYTDLARAVAKLLADAGAV"
     gene            5645..>6213
                     /locus_tag="VHE37_15355"
     CDS             5645..>6213
                     /locus_tag="VHE37_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HVT36966.1"
                     /translation="MTTKLAAFKAYDIRGRVPDELNADLAYRLGIAYAEQFSPKRVAL
                     GRDVRHSSEPLLHALAHGLVSRGVEVLDIGLAGTEEIYWAAQQPGVDGGIEVTASHNP
                     MDYNGMKLVRGGAIPVSGDTGLRELEARVAASGATAPAGKPRMTPADFRGAYVKHLLG
                     YIDAKKLKPLTIVVNAGNGVAGAVIDALEP"
BASE COUNT         1031 a         2109 c         2043 g         1030 t
ORIGIN      
        1 gcatgtaggc gtaggtgttc tgcgccaggt attcgaggcg ttcgcggatc ttgttcgggt
       61 tttccctgtc gcgcaccatg cgcaccgctt ccaccgtgac cacgccctgc gccacgaaca
      121 ggttctggca gtcgatcacg cgcagcgcga acggccccgg cacgccggcc ttggcgcgga
      181 tcggcttgta gtcgttgaga atggccagcg ccgccttggt cgcgttgtcg tagatcgggc
      241 tgcggctgga ggcgatggtc aggcagaaca ccaggtcgta gtcgatcacc agcttggaca
      301 ggaacaggtc cttgatgtgc tcgaccggca gcggcgaact ggaagcgtcg cccctggcgc
      361 ccaggtgttg ctggtagaac gccagcgtcg cctcggcgtt gcgcttgtcg acgatgtcgg
      421 catcgcccag gtgaatggtg atcggcagga tcacgatccg gttgtcctcg agaaattccc
      481 gcggtaaatc gcaggcggaa tccaccacga taccgatgcg catgtcctct cctcccagct
      541 gccgctttgt tgcggcttga tggcaggact gtagcgccgt cagccgcctt tctccagacg
      601 cttggcctcc tgccacagcg tttccatggc ttcgagccgg ccgggcgcac ccagcggcgg
      661 cagcgtgtgc gcatgacgca tgatgtgctc ccagcgccgc aggaacttgg cgttggcgcc
      721 ggccagcgcg gcgttcgcgt ccactttcag atgacgcgcg aggttcaccg ccatgaagag
      781 caaatcgccc agttcgtcct gtgcgcgctc gcggccttcg ccggcggcga ccttcaactc
      841 gccgatctcc tcggccagct tgtcccacag ctcgtcgagg tggcgccagt cgaagccttc
      901 acgctccgcg atcagctgca cgcgcaaggc atcgaccagc ggatcgccgc tcatttcacc
      961 tgccccactt tctgcgccag gttccacagc atgccggcgg tggctcccca gatgcgccag
     1021 cgctgatagt tgagctcgaa gaacggcacg ttgacgccgc tgcgggtgaa ggccttgcgc
     1081 tcgaagttct ccaccttgag caggaacgac agcggtacct cgaacacttc ggccacttcc
     1141 gattcgtcgt aggtgaagga cgcgctgccg tcgaccatgc cgaccaccgg cgtcacccgg
     1201 tagcgggtga tggtcgggta atcgtcgaga tagccgacca cctcgaccag cttcggatcg
     1261 aggccggttt cctcctgcgc ctcgcgcagc gcattggccg ccgcgctggt gtcttcctcg
     1321 tcgcggcgcc cgcccggaaa actgatctgg cccttgtgcg cgcgcagcgt ctccgcgcgc
     1381 agggtgaaca gcacggtgag ctcgcgcgcg cggcggatca ccggcaccag caccgccgcc
     1441 ggccgcagtg aattgagcag gcgcggcgtg atcagcttgt cgatgttcag cggcagctcg
     1501 attggcgaga tcggacgcgg cggctcctcg ctggtgttgt gcagcgcgcg gcgcaggcgc
     1561 gcttcgagat ccgcaggctt catgccgcta ttgtttcatg ccgcaaatcg cctcaccgca
     1621 cactgctttt tgatgttaat aaatattata tgttttgttt tttacataat gttttataac
     1681 acgccaaacg gcagtgcggt tgagccaaaa aaccgcccgc tacaatgccg catgagcctc
     1741 accaacatcc agggcgcgct gcgcgccaac ttccgccgcg cggtgccgca cgtgcgcgag
     1801 ctcggcatcg aggtcagcat ttgcgagccc gagtacgtcg agatgatgct gccgtaccgc
     1861 gaggactggc tcggcgatgt cgagcgcggc gtgatccaca ccggcgtgat caccacgctg
     1921 gtcgattccg cctgcggcct cgccctgctg gcgcacctgt cgacgctgga gccgatcgcc
     1981 acgctcgacc tgcgcatgga ctacctgcga ccggccttcg cgcccaagcc ggtgtggtgc
     2041 cgcgccgagt gctaccgctc caccgagaac atcgtcttca tgcgcgcatc gtgctggcag
     2101 gatgaccgcg cgcagccggt gtcggcgacg caggccgcgt tcatgcgcac cacgcgctcg
     2161 cgcccgcggc cgaacatgat cccggcgaac gagccatgag ccgcatcagg gcagcgcgcg
     2221 aggcggctca gcgcggcgat cacgcggcgc tggtcgacgc cattccctac gcgaaattcc
     2281 tcggcgtgaa actggtcacc gagggcgcgc gcctgctcag cgtgctgccg ttccgcgccg
     2341 ggctggtggg caaccagacc atcaacgcca tccacggcgg cgtcaccgcc gcgttcatgg
     2401 agaacgccgc actgctgcac ctgctgctgc ggctggacca ggagcgcgtg cccaagagca
     2461 tcgacttcgc catcgactac ctgctgccag gccaggcgct ggacacctac gccgagtgca
     2521 aggtgtcgcg cgccggcgtg cgggtggcgc aggtgcaggt caactgctgg cagaaatcgg
     2581 agcaggtgcc gatcgcggtg gcgcgtgcgc acttcctgct caccgcgccg gaatgagcga
     2641 ttaccgcggc tggatcacgc tgcgcgaaat cgatgcgcgg ctcgggcagt ccaagggcac
     2701 cgccttccgc cgcttcaagc aactggaacc cgcgctgcgc gaaggcgcgg atttcgtcgt
     2761 gctcgatgcg cagcgggatc gggcgcagat cgacgcgctg cgcgcgcagc agcgcattta
     2821 cgcctcgagc atcaacgtga tcctgctgtc gccatcgtca gcgcaacggc tcgccgccgc
     2881 ggcgcagtaa gccccgttac cgcgcattgc cgcgcggccg caccgcgggt agcatgccgc
     2941 ctcctacttt gaaccggagt caaaaatgtc tgcagccacc gatctgctca agaagctgcc
     3001 cgcggcgttc aatcccgatg gcgccagcga cagcaccatc cagttcaaca tctccacgcc
     3061 gatgcacatc gtcatcaagg gcggcgcctg cacgctcaac gacggcgcgg ccgccagccc
     3121 ggatgtgacc atcaccatgg aagacgacga tttcgtcgcg ctgatgaagg gcgagctcaa
     3181 cggcatgacc gcgttcatga ccggcaagct gcagctcgac ggcgacctga tgctggccca
     3241 gcgcatgggc agcatgttcg atcccgccaa gcttggctga tcgcagcaag ccgcaagatc
     3301 aagcccgggg ccgcgaggcc tcgggctttt cttttgcgct cgaggaaaaa gacggccgcc
     3361 tgtggctggc ggcattgcac cggccggagt ggacgccgat ctgcgccgac tggctcaacg
     3421 ccgagatgca cagccgcgtg cgcgccgggc gccggcagct gctggcacgc gcgctggggc
     3481 tggcgaaaaa tcccgaccag gaaattctcg acgctaccgc cggcctgggc cgcgacggct
     3541 ggacgctggc cgcgctgggc gcgcgcgtga cgctgtgcga gcgcacgctg cagctgtacg
     3601 cgctgcagcg cgacgcgcgg gcgcgcgcgt cggcgcaggc gcccgaaatc gcggcgcgcg
     3661 tggagctgct gcaggccgat gcgcacgccg tgctggcggg cgcacgccgc tgggacgccg
     3721 tttacctcga cccgatgtat ccgcatcgcg gcaagagcgc gctgccgcag aaggagatgc
     3781 agctgctgcg cgaactcacc ggcggcgacg atgacgccga cgcgttgctg gcgccggcgc
     3841 tggcctgcgc acgcaggcgc gtggtggtga agcggccgcc gcatgcaccg catctggacg
     3901 ggcgcagacc tgcgctgtcc atggagagcg cgcaggcgcg cttcgacatt tatctcgcgg
     3961 cggtatgatc ggcgcatgcg cctgctctcg ctcgtccgcc acgccaagtc gtcctgggat
     4021 tacgccgaac tctccgactt cgagcgcccg ctcaacgagc gcggccggcg cgacgcgccg
     4081 ctgatggcca agctctgcct gatcgaatgg ggcaagcccg agcggctggt gtcgagcccg
     4141 gcgctacgcg ccatcaccac cgcgcgcacc tttgccgaag tgttcggcat cgccggcgaa
     4201 gaaattattg tgcagccgaa aatctacgag gcggcggtcg agacgctgct gcgcctggtg
     4261 cgcgcgctgg acgatggcga ccgccacgtc atgctgttcg gccataaccc gggcatcagc
     4321 gcgctggccc acgaactggc gcgctgccac ttcgacgaga tgccgacctg cgcggtggca
     4381 ggcttcgaac tggcggtgaa atgctggagc gacgtcggcg ccggctgcgg caagctggtg
     4441 cgctactcca cgcccaaggg atcaagggac tgaatctcag gccggcttca gctgcgcctt
     4501 gaacttcttc gcgttctgca cgtagtgctt gccggacagc gccaccatgg cgatttcctg
     4561 cggcgtcagc gggcgcacga gcttggccgg cgatcccatg atcagcgaac catcgggaaa
     4621 ctgcttgccc tcggtgacca gcgagcaggc gccgacgatg cagttgctgc cgatgcgcgc
     4681 acggttcatc accaggctct tgatgccgat cagcgtgttg tcgccgatgt cgcagccatg
     4741 cagcatgacc atgtgaccaa tggtgcagcc cttgccgatg ttcagcttga tgccgacgtc
     4801 ggtatgcagc accgaaccgt cctgcacgtt ggtgccttcg ccgatgcgga tgtcatcgac
     4861 atcgccgcgc acggtgcagc cgaaccacac gctggattcg tgctccaggg tcaccgagcc
     4921 gatcaccacc gcgttgtccg ccacccacac ctcgccgtta aacttcggca cgcgattttc
     4981 gatggtgtag atcatgcaga agttcctgtc ggcaagggtg aggcgcgcga gtttgacgat
     5041 gatgctcgtc gctgcgcaac tcgcgctgtc ggcgtgcgca tcgaaaccgc cgccgctgcc
     5101 gcgcctggcc agcgatgcgc gcctgctggc attcggcgac agcctgaccg ccggcaccgg
     5161 cgcgacggcg gaacaggcct atccagccgt gctcggcgcc ctgatccacc gcgaggtgat
     5221 caacgccggc gtgccgggcg aaaccagcag cgaaggccgt gtacgcctgc cgcaggtgct
     5281 ggacgacgcg cagccgcagc tggtgatcct gtgcgagggc ggcaacgaca tgctgcgtcg
     5341 gatggacgcg gcgcagatgc gcgccaacct tacggcgatg atccgcgaag tgcagtcgcg
     5401 gcatatcgcg ctggtgctgc tggcagtgcc gcacccggcg atcttcggcc tcaaggccga
     5461 cccggtctac gcatcgctgg cggatgaatt tcacctgccg gtcgagagca gtgcgctgag
     5521 cgcgatcatc ggcgacaacg cgcgcaaggc cgaccagatc cacccgaatg cgcagggcta
     5581 caccgacctg gcacgcgcgg tcgcaaagct gctggcagac gcgggcgccg tttagaatcc
     5641 gcggatgacc acgaaacttg cggccttcaa ggcctacgac atccgcgggc gtgtgcccga
     5701 cgaactcaac gccgacctcg cctaccgcct gggcattgcc tatgccgagc agttttcgcc
     5761 gaaacgcgtg gcgctcggcc gcgacgtgcg ccattccagc gagccgctgc tgcacgcgct
     5821 ggctcacgga ctggtttcac gcggcgtcga ggtgctggac atcggcctgg ccggcaccga
     5881 ggaaatctac tgggcggcgc agcagccggg cgtcgacggc ggcatcgagg tcaccgccag
     5941 ccacaacccg atggactaca acggcatgaa actggtgcgc ggcggcgcca tcccggtatc
     6001 gggcgacacc ggcctgcgcg agttggaagc gcgcgtggcg gcgtcgggcg cgactgcgcc
     6061 ggccggcaag ccgcgcatga cgccggcgga tttccgcggc gcatatgtga agcacctgct
     6121 cggctacatc gatgcaaaga agctcaagcc gctgaccatc gtggtcaatg ccggcaacgg
     6181 cgtggcgggc gcggtgatcg acgcgttgga gcc
//
LOCUS       DASZZC010000723         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_57777,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000723 DASZZC010000000
VERSION     DASZZC010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57777"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..433
                     /locus_tag="VHE37_15360"
     CDS             152..433
                     /locus_tag="VHE37_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36967.1"
                     /translation="MNPSSATSPLNYHRQVPPGGDYNPVSVTPNPYPILADLSVREQA
                     YAAAEKFAADGVKCYVRSWHLGDVGYFVCSTDTYDRLWPDHRAYKPTHA"
     gene            436..966
                     /locus_tag="VHE37_15365"
     CDS             436..966
                     /locus_tag="VHE37_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36968.1"
                     /translation="MRIEPIHRRILDLLENESALTDFQIASRLDAPLPEVRTAVDNLY
                     VDERLTRRTVFGNYLFARSEHAETGAPPDPESNTVPGQLRVHERAKRMRETTQDAFIL
                     MLRRTQGLSTNQLIVQLQISTGRFYEACKVLLSYGLIERGQQGHRARWRLTHLGELEA
                     SNLLNSAMAQSLDRAA"
     gene            968..1678
                     /locus_tag="VHE37_15370"
     CDS             968..1678
                     /locus_tag="VHE37_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36969.1"
                     /translation="MRVVANAIPAKDVLARRALYACAALGFLMVPLSITNPNHTPLTI
                     LAIACAGFTAGVTAVTALMSKTQDESPAAPTPKVRKGERLMILPADFIERSPETIFTL
                     VTLLSKQEGWQSEHARIVSRGLIVIGRYLRYQLQRAELEKADPAMDPDVPRAIHLIAH
                     MLPAYKALALGDTPQMAVERVQGLSPPAAIEAGYAVLEEIIGNAFLPEDPMAKRLATM
                     PPERFDRLLPGLRDVASH"
     gene            1681..2520
                     /locus_tag="VHE37_15375"
     CDS             1681..2520
                     /locus_tag="VHE37_15375"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HVT36970.1"
                     /translation="MAARSTIEWTQSSWNFISGCSRQSPGCLNCYAASIAYRFSGPGL
                     PYEGLARLVNGKPAFTGKVKFNADALNLPRTWKNPRFIFVNSMSDLFHRNVSDDIIDS
                     AFNVMAETPRHTYQVLTKNPQRMIRWLARPFEQTGLTHRPPYSNVILGASIEDAKRAR
                     DRFPNLCLVGEDGWRTMVSMEPLLQAVEIPQRYLNLGPRAWVIVGGESGPGARPFNVE
                     WAEQIVAQCRSASVNVFVKQVGSRALRPTGERLPTSSRKGGDMAEWPEPIRVREMPAP
                     AVA"
     gene            2757..3269
                     /locus_tag="VHE37_15380"
     CDS             2757..3269
                     /locus_tag="VHE37_15380"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HVT36971.1"
                     /translation="MNIAHAQLVPPAQLQMLPPEADDEISVVHAFSLTQPWATLVAIG
                     AKTIETRSWSTPFRGWLAIHAARGFPKACQALCFEEPFKSALAAAGYTHPSQLPRGDV
                     LAIANLIDCISTNRWTPPVETLEYAFGDYSPQRFGWVMPRVRRVQPFKATGALSIWRL
                     PSPITAAQLA"
     gene            3266..3556
                     /locus_tag="VHE37_15385"
     CDS             3266..3556
                     /locus_tag="VHE37_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36972.1"
                     /translation="MSIPADHAQFATEDARERQALAYLRPSLILAALATLAWLTFAVL
                     LPPMLEQRLPANRVIELREFSEQFANLATGALLLACAIVGTVQAWRYHRQST"
     gene            3553..4011
                     /locus_tag="VHE37_15390"
     CDS             3553..4011
                     /locus_tag="VHE37_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36973.1"
                     /translation="MNDSVTQRSRCIWPGCEQPATVSCCEHHLRTLPFHIRAPLLTQQ
                     GATLRARMDADRWIAAYVAGKTLTHPLCSTLGHRLHAESAHYYHVLHCDRCHREVYGD
                     TGWRERIKVSVSITVATIQRRAADVAHWWKCGDCGRHFGRHNETVEHLPF"
     gene            4021..4344
                     /locus_tag="VHE37_15395"
     CDS             4021..4344
                     /locus_tag="VHE37_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36974.1"
                     /translation="MGQTYRLDPSTGRCSIQEPSPAMIAWLRSNVDGKLLIAPVRVMR
                     ACQHRGFVRCLFGRHWRITRAGLNEVDHYDRQAATIALAGAPNARRRNPHVRAAIAKT
                     ARPPP"
     gene            4476..>5224
                     /locus_tag="VHE37_15400"
     CDS             4476..>5224
                     /locus_tag="VHE37_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36975.1"
                     /translation="MNVPSLSACAAQAPSASAKRESTALQAMHLLAMAQMLSASHGTG
                     TELSPVMKRTLHQNAVNLAEEAIAAYQAIDPDLGARLRVNAVTSDPVVHIDAGRYLDL
                     ADADQRPFEACVRIAPTMNPGLPASSHLDISILIPQARDLAALKSFIKLGRYRFDQFC
                     IDNRLSIEATAMPLHSQGMRPAAFFEHFHDTIVMPSLMRAADPDVGALRLYRKATDAS
                     EVMPALLAMIRALAFVSRALDQDPIAIMHLT"
BASE COUNT          925 a         1911 c         1474 g          914 t
ORIGIN      
        1 ctcatcgccc tgctcgtgct cgtgccgctg ttcgatgccg ccgacgaaca gtacggcgcc
       61 gtcacagccg cggccacgat cgtcgcgtgc tttgtcatct actgcttcaa cctggcgcgg
      121 gactccattg cgcccgcggc cgaagactcc aatgaaccca tctagcgcaa cttcaccgct
      181 gaactaccac cgccaggttc cgcccggggg cgattacaac cccgtgagcg tcactcccaa
      241 tccgtacccg attttggccg acctctccgt gcgcgagcag gcttacgccg cagcggaaaa
      301 attcgccgcc gacggcgtca agtgttacgt gagatcgtgg catctcgggg acgtgggcta
      361 cttcgtgtgc tccaccgaca cctacgaccg tctctggccg gatcaccgcg cctacaaacc
      421 cacccacgcc tgaacatgcg catcgaaccg atccaccgcc gcattctcga tctgctcgaa
      481 aacgagtccg cgctgaccga cttccaaatc gcctctcgcc tggacgcgcc gctgccggaa
      541 gtgcgcaccg ctgtcgacaa tttgtacgtc gatgagcgac tgacccgccg aactgttttc
      601 ggcaattatc tttttgcccg cagcgagcac gccgagactg gcgcgccgcc ggaccctgaa
      661 tcaaacaccg tccccggcca gcttcgcgtg catgagcgcg ccaaacgtat gcgcgagacg
      721 actcaggacg cattcatcct gatgctccgc cgcacgcagg ggctttcaac caaccagctc
      781 atcgtgcagc tgcagatttc caccggccgt ttctatgagg cctgcaaggt gctgctgtcg
      841 tatggcctga tcgagcgtgg acagcagggc caccgcgcgc gctggcgcct gacgcatctg
      901 ggcgagctgg aggccagcaa cctgctcaac agcgccatgg ctcaatccct cgaccgggcg
      961 gcctgacatg cgtgtagttg ccaacgccat tcctgccaag gacgtgctcg cgcgccgggc
     1021 gctctacgcc tgcgccgccc tgggctttct catggtgccg ctgtccatca ccaatccgaa
     1081 tcacacacca ctgacgatcc tggccatcgc ctgcgcggga ttcaccgccg gcgtcaccgc
     1141 ggtcaccgcg ctgatgtcca agacgcagga tgaatcgcca gccgcgccca cgcccaaggt
     1201 gcgcaagggc gagcggctga tgattctgcc agccgacttc atcgaacgca gtccagagac
     1261 gatcttcacc ttggtcaccc tgctctccaa gcaggaaggc tggcaatccg agcacgcgcg
     1321 aatcgtgtcg cgcggcctga tcgtcatcgg tcgttacctg cgctatcagc tgcagcgcgc
     1381 cgagctggag aaagccgatc ccgcgatgga ccccgacgtg ccgcgcgcca ttcacctgat
     1441 cgcccacatg ctgccggcct acaaggccct cgcactgggg gacaccccgc agatggccgt
     1501 cgagcgcgtg cagggcctgt ccccgcccgc cgccatcgag gccggatatg cagtgctgga
     1561 agaaatcatc ggcaacgcat tcctgccgga agacccgatg gccaagcgcc tggcaaccat
     1621 gccgccggag cgattcgacc gactgctgcc gggcctgcgc gacgtggcct cgcactgacc
     1681 atggccgcgc gcagcaccat cgagtggacc caaagttctt ggaatttcat ctccggttgt
     1741 tcgcgccagt cgcccggatg tttgaattgt tacgccgcct ccatcgccta tcgcttcagc
     1801 gggcccgggc tgccatacga ggggctcgcg cgtctggtca atggcaagcc cgcattcacc
     1861 ggcaaggtga agttcaacgc tgacgccctg aatctgccac gcacctggaa gaatccgcgc
     1921 ttcattttcg tcaacagcat gtcggatctg ttccatagaa acgtctctga cgacatcatc
     1981 gacagcgcct tcaacgtcat ggcggaaacg cctcggcaca cctaccaggt actgaccaag
     2041 aatccacaac ggatgatccg ttggctcgcg cgaccgttcg aacagaccgg cctcacccac
     2101 cgtccgccgt acagcaacgt gatccttggc gcgagtatcg aagacgccaa gcgcgcccgc
     2161 gatcgttttc ccaacctctg cctggtgggc gaagacggct ggcgcactat ggtctccatg
     2221 gagccgctgc tgcaggccgt cgagattccg cagcgctacc tcaacctggg accgcgtgcg
     2281 tgggtcatcg tcggcggcga atccggccct ggcgcgcgcc cgttcaacgt ggagtgggcc
     2341 gagcaaatcg tcgcgcaatg ccgcagcgcc agcgtcaacg ttttcgtcaa gcaggtgggc
     2401 agccgcgcgc tgcgccccac cggtgaacgc ctgcccacca gcagccgcaa aggcggggac
     2461 atggccgagt ggccagagcc catccgggta cgcgagatgc ccgccccggc cgtcgcttga
     2521 tatcagcggc agacgttgcg attgagtgtt atatattatt taataaatat atatttaata
     2581 aataataaaa aacatgcggt ttgatccggg cgtcccgacc cgggctaccg ccgctgaaca
     2641 cggcgacttc gctgttttgg cctcgggcac ccgccgtccg cgccccgccg ctaaatccgg
     2701 catccccgcc gtttttagcg tcttgcatgt ccaccgctgc accctaagct ggacttgtga
     2761 acatcgccca cgcacagttg gtcccgcctg cgcagctgca gatgctgccg cccgaggccg
     2821 acgacgagat cagcgtcgtg cacgccttct cgctgacgca gccttgggcc acgctggtgg
     2881 ccatcggcgc caagaccatc gagacgcgct cctggtcgac tccgtttcgc gggtggctcg
     2941 ccatccacgc tgcgcggggt ttccccaagg cctgccaggc gctgtgcttt gaagagccgt
     3001 tcaagagcgc gctcgccgcc gcgggttaca cgcatccctc acaactgcct cgcggcgacg
     3061 tgctggccat tgccaacctc atcgactgca tttccaccaa ccgctggacc ccgccggtcg
     3121 aaacactcga atacgccttc ggtgactact ccccccaacg cttcggctgg gtcatgccgc
     3181 gcgtgcgccg cgtgcagccc ttcaaggcta ccggcgcgct gtcgatttgg cgccttccct
     3241 cgcccatcac cgcggcgcag ctcgcgtgag catccccgcc gaccatgccc agttcgccac
     3301 cgaagacgcg cgcgaacgcc aggcgctcgc ttacctgcga ccgtccctga tcctcgctgc
     3361 attggccacg ctggcgtggc tcaccttcgc cgtgctgctg ccgccgatgc tggagcagcg
     3421 cctgccggcc aaccgggtca tcgagctgcg cgaattttcc gagcagttcg ccaacctcgc
     3481 caccggcgcc ctgctcctgg cgtgcgccat cgtcggcact gttcaggcgt ggcgctacca
     3541 ccggcaatcc acgtgaatga ctccgtgaca cagaggtccc gctgcatctg gcccggctgc
     3601 gaacagccag ccaccgtcag ctgctgcgag caccacctgc gcacgctgcc cttccatatc
     3661 cgcgccccgc tgttgaccca acagggcgcc acgctgcgcg cgcgtatgga cgccgatcgc
     3721 tggatcgccg cgtatgtcgc cggcaaaacc ctcacacatc cgttgtgctc gactctgggc
     3781 caccgcctgc acgccgagag cgcgcactac tatcacgtcc ttcactgcga tcgctgtcat
     3841 cgcgaggtct atggcgacac cggatggcgc gagcgcatca aggtctccgt ctccataacc
     3901 gtcgcgacca tccagcggcg cgcagccgat gtcgcgcact ggtggaagtg cggcgactgc
     3961 gggcgccatt tcgggcgcca taacgaaacc gtcgagcatc tgcccttcta gcctctgcag
     4021 atgggccaga cctaccgcct cgacccctcc acggggcgct gcagcatcca ggagccctcg
     4081 ccggcgatga tcgcgtggct gcgctccaac gtcgacggca agttgctcat cgcaccagtt
     4141 cgcgtgatgc gcgcgtgcca gcaccgcggc tttgttcgat gcctgttcgg caggcactgg
     4201 cgtatcactc gcgcaggctt gaatgaagtt gaccactacg accgccaggc cgcgaccatc
     4261 gcgctcgccg gcgcgcccaa tgcccgtcgt cgtaatccac atgtacgcgc agccatagca
     4321 aagacggccc gcccgccgcc gtagggcact tcccgcccca caacccgtcc acgaacatga
     4381 tctacagagg atcggacacg cggcgccgac tcatcagttg catccatgtc gagctgcgcg
     4441 attctggatc catcaaccag gagccacgtc gcaccatgaa cgtccctagc ctctcggcct
     4501 gcgccgccca agcccccagc gcttccgcca aacgcgaatc cacggcgctg caagccatgc
     4561 atctgctggc catggcccaa atgttgtcgg cgtcgcacgg caccggaacc gaactgtctc
     4621 cggtgatgaa gcgcacgctg caccagaatg ccgtcaacct cgccgaagag gccatcgcgg
     4681 cctaccaggc gatcgacccc gacctcggcg cgcgcctgcg cgtgaacgcc gtcaccagcg
     4741 acccggtcgt gcacatcgac gcaggccgct atctcgacct ggcggacgcc gatcagcggc
     4801 cgtttgaggc ctgcgtgcgc atcgccccca cgatgaatcc aggcctgcca gcgtccagcc
     4861 acctggacat cagcatcctg ataccccagg cccgcgacct tgccgcgctg aagtctttca
     4921 tcaagctggg ccgctatcgc ttcgaccagt tctgcatcga caaccggttg agcatcgagg
     4981 ccaccgcgat gccccttcac tcccaaggca tgcggcctgc cgccttcttc gagcacttcc
     5041 acgacaccat cgtcatgccg tccctgatgc gtgccgccga tccggatgtc ggcgcgctgc
     5101 gcttgtaccg caaggcgacc gatgccagcg aagtgatgcc cgccctgttg gcgatgatcc
     5161 gtgccctggc tttcgtcagc cgcgcgctcg accaggatcc gatcgcgatc atgcacctga
     5221 ccag
//
LOCUS       DASZZC010000724         5558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1214649,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000724 DASZZC010000000
VERSION     DASZZC010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214649"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHE37_15405"
     CDS             <1..223
                     /locus_tag="VHE37_15405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVT36976.1"
                     /translation="SLQQVIGRVGRTGRCSGPHLDFEVYQNGLRVDPLPYLAGRAAQP
                     GDQPGTGDVAAADIETEFPAGAGSVAGGF"
     gene            257..808
                     /locus_tag="VHE37_15410"
     CDS             257..808
                     /locus_tag="VHE37_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVT36977.1"
                     /translation="MQKLPREFYDRDTVQVARELLGKLLVHRVDGVERVGRIVETEAY
                     LGPHDQAAHSSRGLTPRTKIMFGPPGHVYVYLVYGMHFCMNLVTQREGMASAVLLRAL
                     EPVQHLALRTQGPALLCKAMGVTLAHNGMDATGRELFVAAPAQPQRLVIVRRPRIGVD
                     YAGHWARRLLRFYIKGNPYVSKP"
     gene            complement(841..1260)
                     /locus_tag="VHE37_15415"
     CDS             complement(841..1260)
                     /locus_tag="VHE37_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HVT36978.1"
                     /translation="MKYVHTMVRISDPAKSLAFYVDALGLKETRRKDYEQGRYTLIFL
                     AAPGDESSEIELTHNWDAEELKVGRGFGHLAYRVDDIYAACQRLADHGVVINRPPRDG
                     RMAFVRSPDGISIELLQQGAPLPPREPWASMPNTGSW"
     gene            1407..2186
                     /locus_tag="VHE37_15420"
     CDS             1407..2186
                     /locus_tag="VHE37_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HVT36979.1"
                     /translation="MPFQTDSSAPAARPRRFLLLLSSAALLALAGCAGQDINQSMAQV
                     LGAMQQTGMAGALSESDIVKGLREALAQGTTRAINQLGRTDGFWGDASVRIPLPDNIA
                     RFEKTLRQFGQGAAVDEFHLTLNRAAEQAVPQVADIFGNAVRQMSIDDARNILAGSDD
                     AATQFFRRTESDALYARILPIVSNATARVGVTQKYKAMIASYGPMLQIAGVKNTDLDA
                     YVTGKALDGLFSGIAAEEARIRHDPAARTSDILRRVFAGSR"
     gene            2254..2664
                     /locus_tag="VHE37_15425"
     CDS             2254..2664
                     /locus_tag="VHE37_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36980.1"
                     /translation="MEQGPLTTEQALELHRSFLKEVLAEMLQALSEGEPGIERVCRSL
                     EVYWESAYARREARRAVLAATTGTAIESTVEPMGRPFQLMVEAELSAAHCADAHMLSQ
                     FVYQEARGIAVEEAMADARKPQRRRRLLERIRQG"
     gene            2875..3111
                     /locus_tag="VHE37_15430"
     CDS             2875..3111
                     /locus_tag="VHE37_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36981.1"
                     /translation="MARSEAGADLPALARQLGVPPARVEFLASLSDAQRRQLAADLER
                     AREQHARHIRGLMDEALNHLPWLLRAPIKKLFGG"
     gene            3116..3901
                     /locus_tag="VHE37_15435"
     CDS             3116..3901
                     /locus_tag="VHE37_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36982.1"
                     /translation="MNIDRDTELRRLASALHRQPGELLYLKPLDGQGLLQLRTLLQNR
                     LLDQFSHLFEKMAASGRIAPDALSALLCRKVFGPSITANMSYYVPADRALRLCKHFDV
                     PFMTDIAREMIPERAAELLRGLPTSLMQGVMRELLLKRDFLTMGGFTDHLPEDKALAL
                     MRQIEKPADCIRISRYAQRKDRVARLTAQMDDAQLTRLIEAAFADQEFVHEIGLVTAE
                     MGDGDQRRMARLTDRIDRVWRKRWRDAAEANGTLDRLQAYFSA"
     gene            complement(3898..4581)
                     /locus_tag="VHE37_15440"
     CDS             complement(3898..4581)
                     /locus_tag="VHE37_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HVT36983.1"
                     /translation="MNATYYDILEVSPAASPETIRAAYRSLMQKHHPDRSGSKERALA
                     LNVAYETLADTQRRMAYDVQIRSQLPALAAVPVYVPETPRRRRRWPWIAALCTLPLLA
                     TGVYLANRPASGLMVVGTAPIEGDAEGLQVLIDLGSIRRAGDEAQMVMLFKPRDGKPF
                     MLAGEPVVGASQVVAYQCRRRTYRSWQKKLVMADGTGVPLPDAHGEVAPSSAHDVALR
                     RGCAAWWER"
     gene            complement(4627..5082)
                     /locus_tag="VHE37_15445"
     CDS             complement(4627..5082)
                     /locus_tag="VHE37_15445"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HVT36984.1"
                     /translation="MLESLGGTDVSHVIQLAVAPVFLLAGIGSILGVMTNRLARVVDN
                     ARVLEGRLPSSTPEQQAHLHARLRTLAQRAKLISRAIALCTLTALLVCATIILLFVGA
                     FFGVNAALPAALLFISALISFASALVMFLREIFLATANLRIGAGEHIQR"
     gene            complement(5354..>5558)
                     /locus_tag="VHE37_15450"
     CDS             complement(5354..>5558)
                     /locus_tag="VHE37_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36985.1"
                     /translation="IVEALMLRAGLTGEGLHYTFEVIAAVAVLMVIVHLWGALQRVQP
                     VSETLEIGMYSGLMLLALLFWPV"
BASE COUNT          926 a         1873 c         1892 g          867 t
ORIGIN      
        1 gtccctgcaa caggtcatcg gccgcgtcgg ccgaaccggc cgctgcagcg ggccgcacct
       61 ggatttcgag gtgtaccaga acggcctgcg cgtcgatccg ctgccctatc tggccgggcg
      121 tgccgcgcag ccgggcgacc agccgggtac cggcgacgtc gccgccgccg atatcgaaac
      181 ggaatttcca gcaggcgcgg gcagcgtcgc cggcggtttc tgacgcctgg ccgccatgcg
      241 cgctaggctc gcgcgcatgc aaaagcttcc gcgcgagttc tacgaccgcg acaccgtgca
      301 ggtggcgcgc gaactgctcg gcaagctgct ggtgcatcgc gtggatggcg ttgagcgcgt
      361 cggccgcatc gtcgagaccg aggcctacct cggcccgcac gaccaggcgg cgcattcctc
      421 gcgcgggctg acgccgcgca cgaaaatcat gttcggtccg ccggggcacg tttacgtgta
      481 cctggtctac ggcatgcatt tctgcatgaa cctggtgacg cagcgcgaag gcatggcctc
      541 cgccgtgctg ctgcgcgcgc tggagccggt gcagcacctg gcgctgcgca cgcaggggcc
      601 ggcgctgctg tgcaaggcca tgggcgtgac gctggcgcac aacggcatgg atgccaccgg
      661 ccgcgagctg ttcgtcgccg cgccggcgca gccgcagcgc ttggtcatcg tgcggcggcc
      721 gcgcatcggc gtggactacg ccggccactg ggcgcggcgc ctgctgcgct tctacatcaa
      781 gggcaatccg tacgtatcga agccttgaac tgcgtgctac gtttttcgta gcaggcggcg
      841 ctaccagctg ccggtgttgg gcatcgaggc ccagggttcg cgcggcggca gcggcgcgcc
      901 ctgctgcagc agctcgatgg agatgccgtc gggcgagcgc acgaaggcca tgcggccgtc
      961 gcgcggcggg cggttgatga ccacgccgtg atcggccagc cgctggcagg cggcgtagat
     1021 gtcgtcgacg cggtaggcca ggtggccgaa gccgcggccc accttcagtt cttccgcatc
     1081 ccagttgtgg gtgagttcga tctcgctgga ctcgtcgccg ggcgcggcca ggaagatcag
     1141 cgtgtagcgg ccctgctcgt aatccttgcg ccgcgtttcc ttcaggccga gcgcatcgac
     1201 gtagaaggcc agcgacttcg ccgggtcgct gatgcgcacc atggtgtgca cgtatttcat
     1261 gtcgtttccg tctgacagca gccgccacgc gcggcgttgt attgtgcgcc acgggtgata
     1321 ccgcaacgct gttttgcgtg ttataaaaaa ttctactaaa tttacttaat acgataaaaa
     1381 caaacatgac caaccggggt gcggtgatgc cattccagac ggattcctca gcgcccgcgg
     1441 cgcgcccgcg ccgctttctc ctgctgctgt ccagcgcagc gctgctggcg ctggccggct
     1501 gcgctgggca ggacatcaac cagagcatgg cgcaggtgct cggcgccatg cagcagaccg
     1561 gcatggccgg tgcgctgtcc gagtccgaca tcgtcaaggg cctgcgcgag gcgctggcgc
     1621 agggcaccac gcgcgccatc aaccagctgg gccgcaccga tggcttctgg ggcgacgcca
     1681 gcgtgcgcat cccgctgccg gacaacatcg cgcgcttcga gaagacgctg cgccagttcg
     1741 gccagggcgc ggcggtggat gaattccatc tcacgttgaa ccgcgccgcg gagcaggcgg
     1801 tgccgcaggt ggccgacatc ttcggcaacg cggtgcggca gatgagcatc gacgatgcgc
     1861 gcaacatcct ggccggttcc gacgatgccg ccacgcagtt cttccgccgc accgaaagcg
     1921 atgcgctgta cgcgcgcatc ctgcccatcg tcagcaatgc caccgcgcgc gtgggcgtga
     1981 cgcagaaata caaggcgatg atcgcctcgt acgggccgat gctgcagatc gccggcgtga
     2041 agaacaccga ccttgacgcc tacgtgaccg gcaaggcgct cgacggcctg ttcagcggca
     2101 tcgccgcgga agaggcgcgc atccgccacg acccggccgc gcgcaccagc gacatcctgc
     2161 gccgcgtgtt cgccggcagc cgctgacagg gctgcccaaa tgaaccgggg aggcgtaagg
     2221 ttgagcgagt cgtctgcgca cgggggtgcg gcgatggagc agggaccact gacgaccgaa
     2281 caggcgctgg aactgcaccg cagcttcctc aaggaagtgc tggcggaaat gctgcaggcg
     2341 ctgtcggaag gcgagcccgg catcgagcgc gtgtgccgtt cgctcgaggt ctactgggaa
     2401 agcgcctacg cccgccgcga ggcgcgccgc gcggtgctcg ccgccaccac cggcaccgcc
     2461 atcgaatcca ccgtcgagcc gatgggccgg ccgttccagc tgatggtgga agccgagctc
     2521 agcgccgcgc attgcgccga cgcgcacatg ctgtcgcagt tcgtctacca ggaagcgcgc
     2581 ggcatcgcgg tggaagaagc gatggccgac gcgcgcaagc cgcagcgccg ccggcgcctg
     2641 ctcgagcgca tccgccaggg ttgagccacg gctccgcaat cccgcgattc cagcaggcgc
     2701 gttccgcgcg ggattttcca tggcccgcga caagcgcatg cagagccagc cagccgccac
     2761 cgcatgctcc tttcgcgtgt tataaaatat aattcaatag ttaattaaat atataattaa
     2821 taacatgcaa aatgccggtg cggtggaagg tttttcgatc ggaaggaacg gctgatggcc
     2881 aggtccgaag caggcgccga tcttcctgcg ctcgcgcgcc agctcggcgt gccgccggcg
     2941 cgcgtggaat tcctggcttc gttgtccgac gcgcagcgca ggcagctggc cgccgacctc
     3001 gagcgcgcgc gcgagcagca cgcgcggcat atccgcgggc tgatggacga agcgctgaac
     3061 cacctgccgt ggctgctgcg cgcgccgatc aagaagctgt tcggcgggtg aggccttgaa
     3121 catcgaccgc gacaccgagc tgcgccggct ggccagtgcg ctgcatcggc agccgggcga
     3181 actgctctac ctcaagccgc tcgacggcca gggcctgctg cagttgcgca cgctgctgca
     3241 gaaccgcctg ctcgaccagt tcagccattt gttcgagaag atggcggcga gcgggcgcat
     3301 cgcacccgat gcgctgtcgg cgctgctgtg caggaaagtg ttcggcccca gcatcaccgc
     3361 caacatgagc tattacgtgc cggccgaccg cgcgctgcgc ctgtgcaagc acttcgacgt
     3421 gccgttcatg accgacatcg cgcgcgaaat gattcccgag cgcgcggccg aactgctgcg
     3481 cggcctgccc acctcgctga tgcagggcgt gatgcgcgag ctgctgctca agcgcgactt
     3541 cctcaccatg ggcggcttca ccgatcacct gcccgaggac aaggcgctgg cgctgatgcg
     3601 ccagatcgaa aagcccgccg actgcatccg catctcgcgc tacgcgcagc gcaaggaccg
     3661 cgtggcgcgg ctcaccgcgc agatggacga cgcgcagctc acgcgcctga tcgaggccgc
     3721 cttcgccgac caggaattcg tccacgagat cgggctggtc accgccgaga tgggcgacgg
     3781 cgaccagcgc cgcatggcgc ggctcaccga ccgcatcgac cgggtgtggc gcaagcgctg
     3841 gcgcgatgcc gcggaggcca acggcacgct cgatcgcctg caggcgtact tttccgccta
     3901 gcgttcccac cacgcagcgc agccgcggcg cagcgccacg tcatgcgcgg acgagggcgc
     3961 cacctcgcca tgcgcatcgg gcagcggcac gccggtgccg tcggccatca ccaacttttt
     4021 ctgccagctg cggtaggtgc gccgccggca ctggtacgcc accacctgcg aggcgccgac
     4081 cactggttcg ccggccagca tgaatggctt gccgtcgcgc ggcttgaaca gcatgaccat
     4141 ctgcgcctcg tcgccggcgc ggcggatgct gcccaggtcg atcagcacct gcaggccttc
     4201 ggcatcgccc tcgatcggcg cggtgcccac caccatcagc ccgctggccg gacggttggc
     4261 caggtagacg ccggtggcga gcagcggcag cgtgcacagg gcggcgatcc acggccagcg
     4321 ccgccggcgg cgcggcgttt ccggcacgta caccggcacc gccgccagcg ccggcagctg
     4381 cgagcggatc tgcacgtcat aggccatgcg ccgctgcgtg tcggccagcg tctcgtaggc
     4441 cacgttgagc gccagcgcgc gttccttcga gccgctgcgg tccgggtgat gcttctgcat
     4501 caggctgcga taggccgcgc ggatggtctc cgggctcgcc gccgggctga cttcgaggat
     4561 gtcgtagtag gtggcgttca tggccgcgca tggttagcgg ccgtggcggg aaaaacttga
     4621 gccgcgctag cgctggatat gctcgccggc gccgatgcgc aggttggcgg tggccagaaa
     4681 aatctcgcgc aggaacatga ccagcgcgga ggcgaacgag atcagcgcgg agatgaacag
     4741 cagcgccgcg ggcagggcgg cattgacgcc gaagaaggcg ccgacgaaca gcaggatgat
     4801 ggtggcgcag accagcagcg cggtgagcgt gcacagggcg atggcgcggc tgatgagctt
     4861 ggcgcgttgc gccagtgtgc gcagccgcgc gtgcaggtgc gcctgctgct ccggtgtgga
     4921 tgaaggcagc cgtccttcga gcacgcgcgc attgtcgacc acgcgcgcca ggcggttggt
     4981 catgacgccg aggatcgagc cgatgccggc cagcaggaac accggcgcca ccgccagctg
     5041 gatcacgtgt gaaacatcgg tgccgccgag gctttcgagc atggtgctag ggcctgttaa
     5101 cgccaacttc gcgcgagcgt tgctgcgcca aaaagcgcca ggaaaggcgc gaggagcgcg
     5161 gtttggttgt tccaagcaag cgacgagcaa cgccgcatgg cactttttgg cgcgcaaccc
     5221 ttcgggacgg gagccatttt gcgcattgcg gtgccactcg tcgctccagt agttcgacta
     5281 cagcacactc ctcgttcctg gcactgcgca tagtggctct ccgccgctcg cgtgaagttg
     5341 gcgttaacag gccctacacc ggccagaaca gcagcgcgag cagcatcagg ccgctgtaca
     5401 tgccgatctc cagcgtttcg ctcaccggct gcacgcgctg cagcgcgccc cacaggtgca
     5461 cgatcaccat cagcacggcg acggcggcga tcacttcaaa ggtgtagtgc aggccctcgc
     5521 cggtgaggcc ggcgcgcagc atcagcgctt ccacgatc
//
LOCUS       DASZZC010000725         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1214655,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000725 DASZZC010000000
VERSION     DASZZC010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214655"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="VHE37_15455"
     CDS             complement(<1..1609)
                     /locus_tag="VHE37_15455"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVT36986.1"
                     /translation="MILLLLLVAFRSMRAVLAAAATVGTALLWTMGAAVLLRIPMNLV
                     TAIVPPLVITLGLSYSIYLLSAYFGALREESLTDQRSRVMWTIGRASTGLVLSAGTTV
                     GSFMALLLNALPAIRHFAVLASIGSAIGVLLSLTYMPALLTIVGTSRARNVAVEKMFE
                     RWGGRLADFDQSWRAWIIAIALIMIPLDLIFAARIHAGSEFIGSFNERAQVRQDFEAI
                     NRAFNGANAMSILVETYVDDALTDPERIRAIETLQEWLRQQPEVGSVISYVDHLKLIN
                     QSLNGNDPKFYAIPADAPAVKQMLVFAGSDDIKHVIDTRFRTALISLRINVDDSVAIA
                     ALVKRLEERLAQLPPPLKAQVTGNPVLATRAVQEIASGQVESIAIATVFIWLLLALMF
                     TSARAGFIALLPTVVPVAIYFGTLGVLNISLSPTTCLIACLVIGIAVDDTIQYLARFN
                     ADARATGSEAGAVKTGLATVLRPITLSTVALCLGFLMFTWSELKSQAQFGMLSAFTLA
                     MAWFMNITLTPALSSRLRIVTLWDLVRL"
BASE COUNT          292 a          531 c          562 g          237 t
ORIGIN      
        1 ccagccgcac caggtcccag agagtgacga tgcgcaggcg agacgacagc gccggcgtga
       61 gcgtgatgtt catgaaccag gccatcgcca gggtgaacgc ggacagcatg ccgaactgcg
      121 cctggctctt gagttcgctc caggtgaaca tgaggaagcc caggcacagc gccacggtgg
      181 acagcgtgat cggccgcagc accgtggcca gcccggtctt caccgcgccc gcctcgctgc
      241 cggtggcgcg cgcgtcggcg ttgaaacgcg ccaggtactg gatggtgtcg tccaccgcga
      301 tgccgatgac caggcaggcg atcaggcagg tggtcggcga cagcgagatg ttgagcacgc
      361 ccagcgtacc gaagtagatc gccaccggga cgaccgtggg cagcagtgca atgaagccgg
      421 cgcgcgcgct ggtgaacatc agcgccagca gcagccagat gaagaccgtg gcgatggcga
      481 tggattccac ctgcccgctg gcgatctcct gcaccgcgcg cgtggccagc accgggttgc
      541 cggtgacctg cgccttgagc ggcggcggca gctgcgccag ccgctcttcc agccgcttga
      601 ccaatgcggc gatggccacc gagtcgtcca cgttgatgcg caggctgatc agcgcggtgc
      661 ggaagcgcgt gtcgatcacg tgcttgatgt cgtcgctgcc ggcgaacacc agcatctgct
      721 tgaccgccgg cgcgtcggcc gggatggcgt agaacttcgg atcattgccg ttgaggctct
      781 ggttgatcag cttgagatga tcgacgtagg aaatcaccga gcccacttcc ggctgctgcc
      841 tgagccactc ctgcagcgtc tcgatggcgc ggatgcgctc gggatcggtc agcgcgtcgt
      901 cgacgtaggt ttccaccagg atggacatgg cgttggcgcc gttgaaggcg cggttgatcg
      961 cctcgaagtc ctggcgcacc tgcgcgcgct cgttgaagct gccgatgaat tcgctgccgg
     1021 cgtggatgcg cgcggcgaat atgagatcca gcgggatcat gatcagcgcg atggcgatga
     1081 tccacgcgcg ccagctctgg tcgaagtcgg ccagccggcc gccccagcgc tcgaacatct
     1141 tttccaccgc cacgttgcgc gcgcgcgagg tgccgacgat ggtcagcagc gccggcatgt
     1201 aggtgagcga cagcagcacg ccaatggccg agccgatcga ggccagcacg gcgaagtggc
     1261 ggatggccgg cagcgcgttg agcagcagcg ccatgaacga gcccacggtg gtgccggccg
     1321 acagcaccag cccggtggag gcgcgtccaa tcgtccacat cacgcgcgag cgctggtcgg
     1381 tgagcgactc ctcgcgcagc gcgccgaagt acgccgacag caggtagatg ctgtacgaca
     1441 ggcccagcgt gatcaccagc ggcggcacga tggcggtgac caggttcatc gggatgcgca
     1501 gcagcactgc cgcgcccatg gtccacagca gcgcggtgcc taccgtggcc gccgccgcca
     1561 gcaccgcccg catcgagcgg aacgccacca gcagcagcag gagaatcacg ccgaacaccg
     1621 cg
//
LOCUS       DASZZC010000726        10341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_508330,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000726 DASZZC010000000
VERSION     DASZZC010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508330"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VHE37_15460"
     CDS             <1..678
                     /locus_tag="VHE37_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HVT36987.1"
                     /translation="AGGSGITPVYSIIRSVLARGRGRVRLVYANRDENSVIFGKQLSQ
                     LSRDYPERLQVIHWLDRVQGFATQAQLAALAEGWEEAEVFVCGPGVFMDNVAAAMKSA
                     GVPHAQVHIERFVSLPEDADDERPAAVIADGPATEIEAELDGKVYQVQGAPGQLLLES
                     LEAAGIAAPFSCRAGACAACMCHLEEGDIELIHNHVLSDEDMQQGWVLACQAVPASPK
                     VRVKYPS"
     gene            675..2228
                     /locus_tag="VHE37_15465"
     CDS             675..2228
                     /locus_tag="VHE37_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HVT36988.1"
                     /translation="MNHTATTAFIAAVEQHGSRTAIVGEDGTRLSYAELDRVRVDGAR
                     ALIAHGIGPGDRVAVWAQNCAEWVYAGLAIHSAGAVLVPVNTRMRGQEAGYVLEKSGA
                     KILFCAGSFLGQHYPTLLGAHAPASLQKIVVLRDAQAGDFTWQDFLKSASEVSPAVVH
                     RRAGEISPDSTMDILFTSGTTGQPKGVITTHRQNLRVVDEWCRRMRLVPEDQYLIVNP
                     FFHVFGYKVGWLGGLLVGCTIYPHAIFDTRAVMARIQQHRISVLPGPPTLFISLLNDP
                     ERAQYDLTSLRSTITGAASVPPSLVERMRTELTFKVLITGYGLTESCGIVALCYPEDD
                     AQTVALNCGKPIPGTEVICAGEDGKEVPRGQAGEVWVRGYNVMQGYLDNEEATREAID
                     ADGWLHTGDIAVMDERGYLRITDRLKDMYIAGGFNCYPAEIERMIAAHPAVAQVAIIG
                     VPDERLGEVGKAFVVPKPNQTLEPAALIAWCKNEMANYKIPRYVDVVAALPLNPSGKV
                     MKFQLRAQS"
     gene            2282..3148
                     /locus_tag="VHE37_15470"
     CDS             2282..3148
                     /locus_tag="VHE37_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT36989.1"
                     /translation="MNANLKGVVTIVTGASRGAGKGIAQALGAQGATVYVTGRSLKEG
                     DAPLPGTIHSTAEAINKSGGKGIAVAVDHTKDEQVAALFARVQKEQGKLDILVNNATY
                     LHDQLIEKGGFWTKSLDLVNILDVGLRSGYVASWHAAQMMTAAKKGLIVFTSSFGGNC
                     YMHGAAYGAQKAGIDRFAYDMGHDLKPYNVAAVSIWMGMLKTDRTRRVMDQDPAKYQG
                     FWDMAETPEFTGDVIAALWRDAKLMEKSGKVLIAAEAAQELGVTEAGGKQPPSHRAFL
                     GGPAAVNPAIVE"
     gene            3145..4581
                     /locus_tag="VHE37_15475"
     CDS             3145..4581
                     /locus_tag="VHE37_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HVT36990.1"
                     /translation="MKRRAAALAGLLLAACSSTTPPATENVATDSSLLQRDGRWMVDS
                     DHRVHLLHGVNAVWKVAPYAPSANADGFTAADADFLAANGFNAVRLGVLFAGVMPQQG
                     VLDASYLDQVDRVVQLLASRHIWVLLDFHQDMLNEKYQGEGFPAWAVDDGGLPNDARE
                     GFPADEFVSLALNTAFDNFWSNKNAVWTPYDQALAAAAQRWHNQPYLLGYDMFNEPWP
                     GTQWPTCFEMDCTRFDATLQQFYTQALAAVRSADAQHLAFFEPQQLFDFGAPSGYGPV
                     ADANIGLSWHDYCSVTLTASANLPVELPDCTIIEPRTMNNADNQISAMGATTLITEFG
                     ATDDTTDIGRVADLADQHLTGWTYWAYKNWGDPTGGAVEGLFTDDSDLTTLKSAKADV
                     LIRPYAQAVAGVPTAMSFDATGKTFALTYTPRATTLPTEIFVPARHYPAGYQAQVTGG
                     HVTSPDNATLLEVVADGSSAVQVTVTPR"
     gene            complement(4717..6069)
                     /gene="mpl"
                     /locus_tag="VHE37_15480"
     CDS             complement(4717..6069)
                     /gene="mpl"
                     /locus_tag="VHE37_15480"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HVT36991.1"
                     /translation="MHIHILGICGTFMAGIAAIAREAGHRVTGSDANAWPPMSTQLEA
                     LGIELQRGYEPAHLQPAPDMVVVGNVVSRGNPAMEYVLNMGLPYISGPQWLAEHVLAG
                     RHVLAVAGTHGKTTTTSLLAWLLEHAGLQPGFLVGGMPQNFGVSARLGRGKYFVIEAD
                     EYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLAAIERQFHHLVRTVPGQGRLI
                     VNGRDAALARVLAQGCWSELDFFDDARGWNARDAAGGFELTVKGESHGVVHTPLAGHH
                     NRSNTLAAFAAAAHADVSIEQCKAALAEFRSVKRRLEKIGEARGVTVYDDFAHHPTAI
                     EVTIDALKRQGGGRILAVLEPRSNTMRLGGHAEELAHSLRDAALCFVYARHDLKWDAR
                     SALAGVGARLRLHAELPALVDDIARAAQPGDRVLVMSNGDFGGVHGKILAALGGGAAP
                     "
     gene            complement(6077..6646)
                     /locus_tag="VHE37_15485"
     CDS             complement(6077..6646)
                     /locus_tag="VHE37_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT36992.1"
                     /translation="MFAQIYGVTLRLLLLRAGPQDLPYAPELTPALLALATALAYATF
                     ALVTPGAPAVFAAAASCAGVWLLARELLKARKLSNRFAQTLHGLLLVAIALGLVTLWP
                     FSKIAPLLVAAASDSGVLEHPERLDLPQLPMLALNLLAFWKLAANAHVLRNAIDSGFG
                     AGLLLALLLEFVQLSMMMFGALLARVLFG"
     gene            6688..7797
                     /gene="rnd"
                     /locus_tag="VHE37_15490"
     CDS             6688..7797
                     /gene="rnd"
                     /locus_tag="VHE37_15490"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HVT36993.1"
                     /translation="MSTPPPVPYQLIDQPEALAEQARAWATRPFLAVDTEFVRVDTYY
                     PILCLVQVDDGERSCCIDMLKIGDAAPLFEVLAQPRILKVFHAAGQDLEILVQLTGAC
                     PAPMFDTQIAATLLGMGDQLGYAGLIQRMLGIEVDKSLSRTDWKRRPLTAPELAYAAA
                     DVRHLAEIYPSLHEQLAACGRLAWLAEDCARQSEPGQYRNDPRDAWRRLKGLARLGAQ
                     EQVVAAALAQWRENEAQARDWPRKWIVEDDAIYRVAQRLPASLAELEELRVLPPRTLA
                     RHGEALLEITRQARSAPPQVLSTRDGLDSAQKTLLQKLQDALRASADAAGLPASYIAP
                     RADLTELVREGARAQVPLLQGWRRELAGEKLLALI"
     gene            complement(7806..8447)
                     /locus_tag="VHE37_15495"
     CDS             complement(7806..8447)
                     /locus_tag="VHE37_15495"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HVT36994.1"
                     /translation="MRIVLLGAPGSGKGTQAKKLMERFKIPQVSTGDLLRAAVAGGTE
                     LGKKAKVAMDAGQLVADDIVIGMIKERLEQEDTHHGFIFDGFPRSIPQAEALDTMLAG
                     LNKPLQTALLLHVEFDALFKRLTGRRTCKVCGQMYNIYYSAPKKDGVCDKEGGELMQR
                     ADDNEDTISKRLKVYREQTEPLIAYYKAQKKFQTVEGEGSMDKIFDDIIDALS"
     gene            8600..9865
                     /locus_tag="VHE37_15500"
     CDS             8600..9865
                     /locus_tag="VHE37_15500"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HVT36995.1"
                     /translation="MPRNAFYAQSGGVTAVINVSAAGVIETARRHADRIGKVYAGRNG
                     IIGALTEDLIDTSLESDAAIAALRHTPAGAFGSCRYKLKGFDKNQAEYERLIEVFKAH
                     DIGYFFYNGGGDSADTCLKISQLGEKMGYPIQAIHVPKTMDNDLPLTDTCPGFGSVAK
                     YTAVSIREASFDLASMAKTSTKVFILEVMGRHAGWTAAAGGLAAEHEGEGPQVILFPE
                     IVFEQDKFLAKVDATVKRHGWCTIVASEGIRNPDGKFVADAGTVDAFGHTQLGGVAST
                     LATLIKDQLKLKYHYAIADYLMRAARHIASKTDLEQSYAVGRHAVELALAGKSGVMAS
                     IVRKSDAPYVWEVGDAPLDQVANTEKKMPRDYISDDGFHITGKCRAYLSPLIQGEDYP
                     PYARGLPQYVTLKNAPVAKKTGRTFEVKK"
     gene            10024..>10341
                     /locus_tag="VHE37_15505"
     CDS             10024..>10341
                     /locus_tag="VHE37_15505"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HVT36996.1"
                     /translation="MFLAQNAIWIALVCAAAAVVYGIVSIKWIMAKSPGNARMVEIAA
                     AVQEGAMAYLNRQYTTIAIVGAVLFVAIGLALGANSWYTAVGFLIGAVLSGATGYIGM
                     NVSV"
BASE COUNT         1788 a         3524 c         3405 g         1624 t
ORIGIN      
        1 gccggcggca gcgggatcac gccggtgtac tcgatcatcc gcagcgtgct ggcgcgcggg
       61 cgcggacgcg tgcggctggt ctacgccaac cgcgacgaga attccgtcat cttcggcaag
      121 cagctgtcgc aactgtcgcg tgactatccc gagcgcctgc aggtgatcca ctggctagac
      181 cgcgtgcagg gtttcgccac gcaagcgcag ctggcggcgc tggccgaggg ctgggaagag
      241 gccgaggtgt tcgtctgcgg ccccggcgtg ttcatggaca atgtggccgc ggcgatgaaa
      301 tccgccggcg tgccgcatgc acaagtacac atcgagcgct tcgtctcgct gccggaagat
      361 gccgacgacg aacgcccggc cgctgtcatc gccgacggtc ctgctacgga aatcgaagca
      421 gagctcgacg gcaaggtcta ccaggtgcag ggcgctccgg gccagctgct gctggagtcg
      481 ctggaagctg cgggcatcgc cgcgccgttc tcctgccgcg ccggcgcctg cgccgcctgc
      541 atgtgccatc tcgaggaagg cgacatcgag ctgatccaca accatgtgct gtccgacgag
      601 gacatgcagc agggctgggt gctggcctgc caggcggtgc cggcttcgcc caaggtccgc
      661 gtgaaatacc cgtcatgaac cataccgcca ccaccgcctt catcgcagcc gtggaacagc
      721 acggcagccg caccgccatc gtcggcgagg acggcacgcg cctctcatat gccgaactgg
      781 accgcgtgcg cgtcgacggc gcgcgtgcgc tgatcgcgca tggcattggc ccgggcgacc
      841 gcgtcgcggt atgggcgcag aactgcgccg agtgggttta cgccgggctt gccatccaca
      901 gcgccggcgc cgtgctggtg ccggtgaaca cgcgcatgcg cgggcaggaa gccggctacg
      961 tgctggagaa aagcggcgcg aagattttgt tctgcgctgg cagcttcctc ggtcagcact
     1021 acccgacttt gctcggcgcg cacgcgcccg cctcgctgca gaagatcgtg gtgctgcgcg
     1081 atgcgcaggc gggcgacttc acctggcagg atttcctcaa gagcgcgagc gaggtttcgc
     1141 ccgcggtggt gcaccggcgc gcgggcgaga tctcgccgga ctccaccatg gacatactgt
     1201 tcacctccgg caccaccggc cagcccaagg gcgtgatcac cacgcaccgg cagaacctgc
     1261 gcgtggtcga cgaatggtgc cgccgcatgc gcctggtgcc cgaagaccag tacctgatcg
     1321 tcaacccgtt cttccacgtg ttcggctaca aggtcggctg gctcggcgga ctgctggtcg
     1381 gctgcaccat ctacccgcac gccatcttcg atacgcgcgc ggtgatggcc cgcatccagc
     1441 agcaccgcat ctccgtgctg cccggcccgc ccactctctt catcagcctg ctcaacgatc
     1501 ccgagcgcgc gcaatacgat ctcacctcgc tgcgctcgac catcaccggc gcggcctcgg
     1561 tgccgccgtc gctggtcgag cgcatgcgca ccgagctcac cttcaaggtg ctgatcaccg
     1621 gctatgggct gaccgaatcc tgcggcattg ttgccttgtg ctacccggaa gacgacgcgc
     1681 agaccgtggc tctcaactgc ggcaagccga tccccggcac cgaggtgatc tgcgccggcg
     1741 aagacggcaa ggaagtgccg cgcggccagg ccggcgaagt gtgggtgcgc ggctacaacg
     1801 tgatgcaggg ctatctcgac aatgaagagg ccacgcgcga agcgatcgac gccgacggct
     1861 ggctgcacac cggcgacatc gcggtgatgg acgagcgcgg ctacctgcgc atcaccgacc
     1921 gcctgaagga catgtacatc gccggcggct tcaactgcta cccggcggag atcgagcgca
     1981 tgatcgccgc gcatcctgcc gtcgcgcagg tggcgatcat cggcgtgccc gatgaacgac
     2041 taggtgaagt cggcaaggcc ttcgtcgtgc ccaagcccaa ccagacgctc gagcccgcgg
     2101 cgctgatcgc ctggtgcaag aatgaaatgg ccaactacaa gatcccgcgc tacgtggacg
     2161 tggtggcggc gctgccgctg aacccgtcgg gcaaggtgat gaagttccag ttgcgcgcac
     2221 aatcttaaat tccctctccc cacttcgtgg ggcgagggag aaaatatttc tggagagaga
     2281 aatgaatgcg aatctgaaag gcgtcgtcac catcgtcacc ggcgcctcgc gcggcgcagg
     2341 caagggcatt gcgcaggcgc tcggagcgca gggcgccacc gtgtacgtca ccggccgctc
     2401 gctcaaggaa ggcgatgcgc ctctgccggg caccatccac tccaccgccg aggccatcaa
     2461 taaatccggc ggcaagggca tcgcagtggc ggtcgaccac acgaaggacg aacaggtggc
     2521 cgcgctgttc gcgcgcgtgc agaaggagca gggcaagctc gacatcctgg tcaacaacgc
     2581 gacctacctg catgaccagc tgatcgagaa aggcggcttc tggaccaagt cgcttgacct
     2641 ggtgaacatc ctcgacgtcg gcctgcgcag cggctatgtg gcgagctggc acgcggcgca
     2701 gatgatgacc gcggcgaaga agggcctgat cgtgttcacc tcctcgttcg gcggcaactg
     2761 ctacatgcac ggcgccgcct acggcgcgca gaaggcgggc atcgaccgct ttgcctacga
     2821 catgggccac gacctcaagc cctacaacgt cgccgcggtg tcgatctgga tgggcatgct
     2881 caagaccgac cgcacgcgcc gcgtgatgga ccaggacccg gccaagtacc agggcttctg
     2941 ggacatggcg gaaaccccgg aattcaccgg cgatgtcatc gccgcgctgt ggcgcgatgc
     3001 gaagctgatg gaaaaatccg gcaaggtgct gatcgctgcc gaggccgcgc aggaactggg
     3061 cgtgaccgag gccggcggca agcagccgcc atcgcaccgc gcgtttctcg gcgggccggc
     3121 tgcggtcaac ccggccatcg tcgaatgaag cgccgcgccg ccgcgctggc cggcctgctg
     3181 ctggcggctt gcagcagcac cacgccgcct gctacggaaa acgtagcaac ggacagcagc
     3241 ctgctgcagc gcgacggccg ctggatggtg gacagcgacc accgcgtgca cctgctgcac
     3301 ggcgtcaacg cggtgtggaa agtggcgccg tacgcgccgt ccgccaacgc tgacggcttc
     3361 accgccgccg atgccgattt cctcgccgcg aatggtttta acgcggtgcg cctgggcgtg
     3421 ctgttcgccg gcgtgatgcc gcaacagggc gtgctcgacg ccagttacct cgaccaggtc
     3481 gaccgcgtgg tgcagctgct ggcctcgcgc catatctggg tgctgctgga tttccaccag
     3541 gacatgctca acgagaaata ccagggcgaa ggtttcccgg cctgggcggt ggacgacggc
     3601 ggactgccca acgacgccag ggaaggattc cccgccgacg aattcgtcag cctggcgctc
     3661 aacaccgcgt tcgacaattt ctggtcgaac aagaatgccg tgtggacgcc gtacgaccag
     3721 gcgctggccg cggcggcaca gcgctggcat aaccagccct acctgctggg ctacgacatg
     3781 ttcaacgagc cgtggcccgg cacgcagtgg ccgacctgct tcgagatgga ctgcacaagg
     3841 ttcgacgcca cgctgcagca gttctacacg caggcgctgg cggcggtgcg cagcgccgat
     3901 gcgcagcacc tggcgttctt cgagccgcag cagctgttcg acttcggcgc gccgagcggc
     3961 tacgggccgg tggccgacgc caacatcggc ctgtcgtggc acgactactg ttcggtcacg
     4021 ctgaccgctt cggccaacct gccggtcgag ttgcccgact gcaccatcat cgagccgcgc
     4081 accatgaaca acgccgacaa ccagatctcg gccatgggcg ccaccacgct gatcaccgag
     4141 ttcggcgcca ccgacgacac caccgacatc ggccgcgtgg ccgatcttgc cgaccagcac
     4201 ctgaccggct ggacctactg ggcctacaag aactggggcg atcccaccgg cggcgccgtc
     4261 gaaggcctgt tcaccgatga cagcgacctg accacgctca agagcgccaa ggccgacgta
     4321 ctgatccgcc cgtatgcgca ggccgtggcc ggcgtgccga cggcgatgag cttcgacgcc
     4381 accggcaaga cctttgcact cacctacacg ccgcgcgcga ccacgctgcc caccgagatc
     4441 ttcgtgccgg cgcgtcacta cccggccggc taccaggcgc aggtcaccgg cggccatgtc
     4501 acttcgccgg acaatgccac gctgctcgag gtggtcgccg acggcagcag cgcggtgcag
     4561 gtgacggtga cgccgcgctg atcgcgtggc atgcgtgtcc ggcaaaaaac catccaccgc
     4621 accggcattt gcgctgttat taattgttat cttaatttaa tttaagacaa ctttttataa
     4681 caccgcaaac cgggatgcgg tgaggcaaaa atcgcgctac ggagccgccc cgccgcccag
     4741 cgccgccagg atttttccgt gcacgccgcc gaagtcgccg ttgctcatca ccagcacgcg
     4801 atcgccgggc tgcgctgcgc gcgcgatgtc gtccaccagc gccggcagtt ccgcgtgcag
     4861 gcgcaggcgc gcgcccacgc cggccagcgc tgaacgcgca tcccacttga gatcgtgccg
     4921 cgcgtagacg aagcacagcg ccgcgtcgcg caggctgtgc gccagttctt ccgcgtgccc
     4981 gcccaggcgc atggtgttgg agcgcggctc cagcacggcg aggatgcggc cgccgccctg
     5041 gcgcttcagc gcatcgatgg tgacttcgat cgcggtcggg tgatgcgcga agtcgtcgta
     5101 gaccgtcacg ccgcgcgctt cgccgatttt ttccaggcgg cgcttcacgc tcctgaattc
     5161 cgccagcgcg gccttgcatt gttcgataga cacgtcggca tgcgcggcgg cggcgaaggc
     5221 ggccagcgtg ttgctgcggt tgtgatggcc agcgagcggc gtgtgcacga caccgtggct
     5281 ttcgcccttc accgtcagtt caaagccacc ggcggcgtcg cgcgcgttcc acccgcgcgc
     5341 gtcgtcgaag aaatccagct cgctccagca gccctgcgcc agcacgcgcg ccagcgcggc
     5401 gtcgcggcca ttgacgatca gcctgccctg ccccggcacc gtgcgcacca ggtgatggaa
     5461 ctgccgctcg atcgccgcca ggtcgggaaa gatgtcggcg tggtcgtact cgaggttgtt
     5521 gaggatcgcg gtgcgcgggc ggtagtgcac gaacttgctg cgcttgtcga agaacgcggt
     5581 gtcgtactcg tcggcctcga tcacgaaata ttttccgcgc cccagccgtg ccgacacgcc
     5641 gaagttctgc ggcatgccgc cgaccaggaa acctggctgc aggcccgcgt gttccagcag
     5701 ccaggcgagc agcgaggtgg tggtggtctt gccgtgcgtg ccggcgaccg caagcacgtg
     5761 gcgcccggcc agcacgtgct cggccagcca ctgcggaccg ctgatgtacg gcaggcccat
     5821 gttcagcacg tattccatcg ccgggttgcc gcgcgacacc acgttgccca ccaccaccat
     5881 gtcgggcgcg ggctgcaggt gcgccggctc gtagccgcgc tgcaattcga tgcccagcgc
     5941 ttccagctgc gtcgacatcg gcggccaggc gttggcgtcg gagccggtga cgcggtggcc
     6001 ggcctcgcgc gcaatcgccg cgatgccggc catgaaggtg ccgcagatgc ccaggatgtg
     6061 gatgtgcatg tcgcgctcag ccgaacagca cgcgcgccag cagcgcgccg aacatcatca
     6121 tcgacagctg cacgaattcc agcagcagcg ccagcagcag gccggcgccg aagccgctgt
     6181 cgatggcgtt gcgcagcacg tgcgcattgg ccgccagctt ccagaaggcg agcaggttga
     6241 gcgccagcat gggcagctgc ggcaggtcca gccgctcggg atgctccagc acaccgctgt
     6301 cgctggcggc ggcgaccagc agcggcgcaa tcttcgagaa cggccacagc gtgaccagcc
     6361 ccagcgcgat cgccaccagc agcaggccgt gcagcgtctg cgcaaaacgg ttcgacagct
     6421 tgcgcgcctt gagcagctcg cgcgccagca gccagacgcc ggcgcagctg gccgcggcgg
     6481 cgaacaccgc gggcgcaccc ggcgtgacca gcgcgaaggt ggcgtaggcc agcgcggtgg
     6541 ccagcgccag cagcgcgggc gtcagctcgg gcgcataggg caggtcctgc gggccggcgc
     6601 gcagcagcag caggcgcagc gtcacgccgt agatctgggc gaacatttcg gatcggagcg
     6661 aagtgaagcc gttattctat cgcccgcatg tccaccccgc cccccgttcc ctaccagctc
     6721 atcgaccagc ccgaggcgct cgccgagcag gcgcgcgcct gggccacgcg cccgttcctc
     6781 gccgtcgaca ccgagttcgt gcgcgtcgac acctattacc cgatcctgtg cctggtgcag
     6841 gtggacgacg gcgagcgcag ctgctgcatc gacatgctga agatcggcga cgccgcgccg
     6901 ctgttcgagg tgctggcgca gccgcgcatc ctcaaggtgt tccacgccgc cggccaggac
     6961 ctggaaatcc tggtgcagct caccggcgcc tgcccggcgc cgatgttcga cacgcagatc
     7021 gccgccacgc tgctgggcat gggcgaccag ctcggctacg ccggcctgat ccagcgcatg
     7081 ctcggcatcg aggtggacaa gtcgctgtcg cgcaccgact ggaagcgccg cccgctgacc
     7141 gcgccggagc tggcctacgc cgccgccgat gtgcgccacc tggcggaaat ctatccctcg
     7201 ctgcacgagc agctggccgc ctgcgggcgg ctggcctggc tggccgagga ttgcgcgcgg
     7261 caatccgagc cggggcaata ccgcaacgat ccgcgcgatg cctggcggcg cctgaaaggc
     7321 ctggcgcgcc tgggcgcgca ggagcaggtg gtggcggcag cgctggcgca gtggcgcgaa
     7381 aacgaggcgc aggccaggga ctggccgcgc aagtggatcg tcgaggacga cgccatctac
     7441 cgcgtcgcgc agcggctgcc ggcgagcctg gccgaactgg aagagctgcg cgtgctgccg
     7501 cccaggacgc tggcgcgcca cggcgaggcg ctgctggaga tcacccggca ggcacgcagc
     7561 gcgccgccgc aggtgctgtc cacccgcgat ggactggaca gcgcgcagaa aacgctgctg
     7621 cagaaactgc aggacgcctt gcgggcaagc gccgacgccg cgggactgcc cgccagctat
     7681 atcgcgccgc gtgccgacct gacggagttg gtgcgcgaag gcgcgcgcgc gcaggtgccg
     7741 ctgctgcagg gctggcggcg cgagctggcg ggagaaaaac tgctggcgct gatctagcgc
     7801 cgggcctacg acagcgcgtc gatgatgtcg tcgaagatct tgtccatcga gccttcgcct
     7861 tccaccgtct ggaacttctt ctgcgccttg taataggcga tcagcggttc ggtctgctcg
     7921 cggtagacct tgaggcgctt gctgatggtg tcctcgttgt cgtcggcgcg ctgcatcagc
     7981 tcgccgcctt ccttgtcgca cacgccgtct ttcttcggcg cgctgtagta gatgttgtac
     8041 atctgaccgc acaccttgca ggtgcggcgg ccggtgaggc gcttgaacag cgcatcgaat
     8101 tccacgtgca gcagcagcgc ggtctgcagc ggcttgttca gcccggccag catggtgtcc
     8161 agcgcctcgg cctgcgggat cgagcgcgga aagccgtcga agatgaagcc gtggtgcgtg
     8221 tcttcctgct ccaggcgttc cttgatcatg ccgatgacga tgtcgtcggc caccagctgg
     8281 ccggcgtcca tggccacctt ggccttcttg cccagctcgg tgccgccggc caccgccgca
     8341 cgcagcaggt cgccggtgga cacctgcggg atcttgaagc gttccatcag cttcttggcc
     8401 tgcgtgccct tgccggagcc aggtgcgccc agcagtacga ttctcatgat tcggatggcc
     8461 ctattggatg cggccgcggg cgggccgcgg gtgcgcaaaa gtaaccgctc gcaccagcgc
     8521 ggtcaatgaa gtttctcctg ccgcgttgtc ctattcctta caattgcggc cgcgctgttt
     8581 tcgcagaccg gaaaaccaca tgccacgcaa cgccttctac gcgcaatcgg gcggcgtcac
     8641 cgccgtcatc aatgtgtccg ccgccggcgt gatcgagacc gcgcgccggc acgccgaccg
     8701 catcggcaag gtttacgccg gccgcaacgg catcatcggc gcgctgaccg aagacctgat
     8761 cgacacctcg ctggagtccg atgccgccat cgccgcgctc aggcacacgc cggccggcgc
     8821 cttcggctcg tgccgctaca agctcaaggg cttcgacaag aaccaggcgg agtacgagcg
     8881 gctgatcgag gtgttcaagg cgcacgacat cggctacttc ttctacaacg gcggcggcga
     8941 ttccgccgac acctgcctga agatttcgca gctgggcgag aagatgggct acccgatcca
     9001 ggccatccac gtgcccaaga ccatggacaa cgacctgccg ctcactgaca cctgccccgg
     9061 cttcggcagc gtggccaagt acaccgcggt gtcgatccgc gaagcgagct tcgacctcgc
     9121 ttcgatggcc aagacctcga ccaaggtgtt catcctggaa gtgatgggcc gccatgccgg
     9181 ctggaccgcg gccgcgggcg gactggccgc agagcatgaa ggcgaagggc cgcaggtgat
     9241 cctgtttccc gaaatcgtgt tcgagcagga caagttcctg gccaaggtcg atgccacggt
     9301 gaagcggcac ggctggtgca ccatcgtggc cagcgagggc atccgcaacc cggacggcaa
     9361 gttcgtcgcc gacgccggca cggtggacgc cttcggccac acccagctgg gcggcgtggc
     9421 ctcgacgctg gccacgctga tcaaggacca gctcaagctg aaataccact acgccatcgc
     9481 ggactacctg atgcgcgcgg cacggcacat cgcgtcgaaa accgatctcg agcagtcgta
     9541 cgcggtgggc cggcacgcgg tggagcttgc gcttgcaggc aagagcggcg tcatggccag
     9601 catcgtgcgc aagtccgacg ctccttatgt ttgggaggtg ggcgacgcgc cgctggacca
     9661 ggtggccaac accgagaaga agatgccgcg cgactacatc agcgacgacg gcttccacat
     9721 caccggaaaa tgccgcgcct acctgtcgcc gctgatccag ggcgaggact acccgccgta
     9781 tgcccgcggc ctgccgcagt acgtcacgct gaaaaacgcg ccggtggcga agaagaccgg
     9841 ccgtactttc gaggtcaaga aataaccagc accgccgatt ttccgtctgc aaatggagta
     9901 gcggccgggc aaaaaccctg tgttatgctc ggccgcgccg caaggcgctg cacataaatt
     9961 gcccacgccg catcgagcgg ccgcagctta cccaaacaga aatacggatc gggaggtcat
    10021 tcgatgttcc tggctcaaaa cgcaatctgg attgcgcttg tctgcgcagc cgccgccgtg
    10081 gtttacggca tcgtttccat caaatggatc atggcgaaat cgccgggcaa cgcgcgcatg
    10141 gtggaaatcg ctgcggcggt gcaggaaggc gccatggcct atctcaaccg ccagtacacc
    10201 accatcgcca tcgtcggcgc ggtgctgttc gtggccatcg gcctcgctct gggcgccaac
    10261 agctggtaca cggcggtcgg tttcctgatc ggcgcggtgc tgtcgggcgc caccggctac
    10321 atcggcatga acgtgtcggt g
//
LOCUS       DASZZC010000727         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_433807,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000727 DASZZC010000000
VERSION     DASZZC010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433807"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..900
                     /locus_tag="VHE37_15510"
     CDS             340..900
                     /locus_tag="VHE37_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HVT36997.1"
                     /translation="MLVDGDKTRSKMLESTLSEHGHSVVARLSAEENLAAAVAQYQPQ
                     VVLLHVDSPGHATLESLGQVHRENPRPVVLFAARSDADTTRRAVQAGVSAYIVDGLQP
                     ARISAVLEVAIARFELHQSMRSELDKARTRLSDSRDIEKAKGLLMKRRGIDEAEAFSL
                     LRRMAMDRQQRLGDVARMLLGAADVI"
     gene            1098..>1559
                     /locus_tag="VHE37_15515"
     CDS             1098..>1559
                     /locus_tag="VHE37_15515"
                     /inference="COORDINATES: protein motif:HMM:NF022677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HVT36998.1"
                     /translation="MNAIKGTRADKLRTVVGAGLLAAGALSSTAAQADWYNDGKVLAT
                     GGVITVDGAGGGGITPWATISGYETKDGINGGLHYTWVYLPDYSLNSYGATVGFYDRL
                     ELSYTNSVLPTGSTFDTVGLLTTTLGSTVGLAGNTGIKPFNTTIKMDVFGAK"
BASE COUNT          302 a          481 c          512 g          264 t
ORIGIN      
        1 cctcgatcga ctcgatgtag ccgagcttga ccagcccctg tgcaaccttg cgcatgtggt
       61 gcgcggacac gccgtatttc tgtgcgatgt gcgtggtgct caccggcgtg ctgcggtcgg
      121 ccgcggccag gtacaacagc aggcgcagcg aaaaatcggt gtagagcgac agcttcatga
      181 tgttctcctc acttgcgtga tgcaccaatt agcaacaaat gcgccataac ccggtgatcc
      241 accatgcgcc agatacatgg cattcaattt gcgaatttga aactcaaaca cgagacggga
      301 gccgggtcga tggggcgcgc cgctaaagga ctccgcatca tgctggtaga cggagacaag
      361 acgcgctcaa aaatgctcga aagcacgctg agcgagcatg gtcatagcgt ggtggcgcgt
      421 ttgtcggcgg aagagaatct ggcggcggcg gtggcgcaat accagccgca ggtggtgctg
      481 ctgcacgtgg actcaccggg acacgccacg ctcgaatcgc ttggccaggt gcatcgcgag
      541 aatccgcggc cggtggtgct gttcgccgcg cgcagcgacg ccgataccac gcgccgcgcg
      601 gtgcaggcgg gcgtcagcgc ctacatcgtc gacggcctgc agccggcgcg catcagcgcg
      661 gtgctcgaag tggccatcgc gcgcttcgag ctgcaccaga gcatgcgctc cgagctggac
      721 aaggcgcgca cgcgcctgtc cgacagccgc gacatcgaga aggccaaggg cctgctgatg
      781 aagcgccgcg gcatcgacga agccgaggcc ttcagcctgc tgcggcgcat ggcgatggac
      841 cggcagcagc gcctgggcga tgtcgcccgc atgctgctcg gcgcggccga cgtcatctga
      901 gctttgtttc agcgccatga attaagtggc atgacgattg cgtagttaac ttcggcgccg
      961 aatacgcgcc gatgcacgcg aggccggcca acgatgcccg gcccaacgga caccgacgtc
     1021 cgcattgctg acaccgccgt cagcagtgcg gacgtttttt ttggcttgca gatttcacga
     1081 ctgaaggagc tttttcgatg aatgcaataa aagggacgcg ggcggacaag ctgcggacgg
     1141 ttgtcggcgc gggcttgctg gcggcgggtg cgctgtcgag tactgcggca caagccgact
     1201 ggtacaacga cggcaaggtg ctggcgaccg gcggcgtaat cacggtggac ggcgcgggcg
     1261 gcggcggcat tacgccgtgg gccaccatct cggggtacga gaccaaggac ggcatcaatg
     1321 gcggcctgca ctacacctgg gtctacctgc cggactacag cttgaactcc tatggcgcca
     1381 cggtcggctt ctatgaccgg ctcgagctgt cgtacaccaa cagcgtgctg cccaccggtt
     1441 ccaccttcga caccgtgggc ctgctcacca ccacgctggg ttcgaccgtg gggctggccg
     1501 gcaacaccgg catcaaaccg ttcaacacca ccatcaagat ggacgtgttc ggggccaag
//
LOCUS       DASZZC010000728         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_57882,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000728 DASZZC010000000
VERSION     DASZZC010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57882"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="lpxK"
                     /locus_tag="VHE37_15520"
     CDS             complement(<1..855)
                     /gene="lpxK"
                     /locus_tag="VHE37_15520"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HVT36999.1"
                     /translation="MSLRRWLEQQWQAQHAPAALRPLSLVYGGVSATRRALLSARTPK
                     LPLPVVVVGNISVGGTGKTPFTVWLVERLRAWGWKPGIVSRGYGGHAPHYPLRVRRET
                     PPEHSGDEPAMLVRRLGCPLYVAPDRVAAARALIGSGEANIIVADDGLQHYRLPRDLE
                     ICIVDGQRRFGNGALLPAGPLREPPRRLAEVNLVVINGSDWACPAPARVRMDLAAREP
                     QPLAGGAPRPWAQFAATRVHAIAGIGNPARFFDTLRAHGLDPIEHAFPDHHEFVTGDL
                     NFGDRLPVL"
     gene            complement(833..2599)
                     /gene="msbA"
                     /locus_tag="VHE37_15525"
     CDS             complement(833..2599)
                     /gene="msbA"
                     /locus_tag="VHE37_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HVT37000.1"
                     /translation="MTDSQTPGALAVYRRLLGYSAPHWRVFLVAVLGMVVFASVDVSF
                     ARLVQPLTDSTFGLKNAREGQMLPWIILGLFVCRGAAAFLSSYGMAWVSQKVVLQLRG
                     EVFDQILHLPVSHHDRVRNADLLVKLTYHVNQVSESASSVITSMIKDSLTIIGLLFVM
                     SYNSWKLTLITLTLVPVVAWSVRYVSKRFKMINQRLQQSMGSVTHVADEAITGRRVVK
                     VYGGEAAEVARFKKVGDYIRVQSMKLVASSSGAGGFVQFIAAFAISLIVFLAGSTDML
                     RQDTAGTFVSFMAALLMMRAPLNSLSGVNERLSRGLTAAADLFAFLDTPREADAGTRT
                     LARAAGAIAFEDVRFAYGEDKGEALAGLSLSIRAGQTVAFVGRSGSGKSTLLSLLPRF
                     YDPTGGRVLLDGVDLREYRLADLRRQIALVDQNIVLFNGTVAENIAYGCRERAADADI
                     ISAAKAAYAWDFIEKLPQGIETQVGQDGLNLSGGQRQRIAIARALYKNAPILILDEAT
                     SALDTESERYIQAGLEQLVQGRTTLVIAHRLSTIQRADLIVVMQQGRIVEQGRHDELL
                     ARGGAYASLHSMQFRDEPAPVA"
     gene            2674..3015
                     /locus_tag="VHE37_15530"
     CDS             2674..3015
                     /locus_tag="VHE37_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HVT37001.1"
                     /translation="MAKTLFEKIIAREIPATFVHEDEHCVAFRDISPAAPTHVLLVPR
                     KPLTMLTDASAEDQLLLGHLMLTASKIARDLGVGDAFRLAVNNGAGAGQSVFHLHLHI
                     LGGRPLKWPPG"
     gene            complement(3020..>3217)
                     /locus_tag="VHE37_15535"
     CDS             complement(3020..>3217)
                     /locus_tag="VHE37_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVT37002.1"
                     /translation="QGRTPLMYAALSRYGDRSLEALIHHSAKINAYDTGGKTALQLIP
                     TQLKGPPAERHYAMLYAAGAR"
BASE COUNT          509 a         1124 c         1121 g          463 t
ORIGIN      
        1 cagcaccggc aggcggtcgc cgaagttgag atcgccggtg acgaattcat ggtgatcggg
       61 aaacgcatgc tcgatggggt cgaggccgtg cgcgcgcagc gtgtcgaaga agcgcgccgg
      121 gttgccgatg ccggcgatgg cgtgcacgcg cgtggcggcg aactgcgccc acgggcgcgg
      181 cgcgccgccg gccagcggct gcggctcgcg cgccgccagg tccatccgca cgcgcgccgg
      241 cgcgggacag gcccagtcgc tgccgttgat caccaccagg ttcacttccg ccagccgcct
      301 gggcggctcg cgcagcgggc cggccggtag cagcgcgccg ttgccgaagc gccgctggcc
      361 atcgacgatg cagatctcca ggtcgcgcgg caggcggtaa tgctgcaggc cgtcatcggc
      421 gacgatgatg ttggcctcgc cgctgccgat cagcgcgcgt gccgcggcca ctcgatccgg
      481 cgccacgtac aacgggcagc cgaggcggcg caccagcatc gccggctcgt cgcccgaatg
      541 ttccggcggc gtctcgcgcc gcacgcgcag cgggtaatgc ggcgcatgcc cgccgtagcc
      601 gcggctgacg atgcccggct tccagcccca tgcgcgcagg cgctcgacca gccacacggt
      661 gaacggcgtc ttgccggtgc cgccgacgct gatattgccg accaccacca ccggcagcgg
      721 aagtttcggc gtgcgcgcgc tgagcagcgc gcggcgcgtg gcggatacgc cgccgtagac
      781 cagcgacagc gggcgcagcg ccgccggcgc gtgctgcgcc tgccattgct gctcaagcca
      841 ccggcgcagg ctcatcccgg aactgcatgc tgtgcagcga ggcataggcg ccgccgcgcg
      901 ccagcagctc gtcgtggcga ccctgctcga cgatgcggcc ctgctgcatc accacgatca
      961 ggtcggcgcg ctggatggtc gacagccggt gcgcgatgac cagcgtggtg cgcccctgca
     1021 ccagctgctc gaggccggcc tggatgtagc gctcggactc ggtgtcgagc gcgctggtcg
     1081 cttcgtcgag gatcaggatc ggcgcgttct tgtacagcgc gcgcgcgatg gcgatgcgct
     1141 ggcgctggcc gccggaaaga ttaagcccgt cctgccccac ctgggtctcg atgccctgcg
     1201 gcagcttctc gatgaagtcc cacgcgtacg ccgccttggc cgcggagatg atgtcggcat
     1261 cggcagcgcg ctcgcggcac ccataggcga tgttctccgc caccgtgccg ttgaacagca
     1321 cgatgttctg gtcgaccagc gcgatctgcc ggcgcaggtc ggccagccgg tattcgcgca
     1381 ggtccacgcc gtcgagcagc acgcgtccgc cggtgggatc gtagaagcgc ggcagcagcg
     1441 acagcagcgt ggacttgccg ctgccggagc ggccgacgaa ggccacggtc tgcccggcgc
     1501 ggatcgacag cgacagcccg gccagcgcct cgcccttgtc ctcgccgtag gcgaagcgca
     1561 cgtcctcgaa agcgatggcg cctgccgcgc gcgccagcgt gcgcgtgccg gcatcggcct
     1621 cgcgcggcgt gtcgagaaag gcaaacaggt cggccgccgc ggtgagcccg cgcgacaggc
     1681 gctcgttcac gcccgacagc gagttgagcg gcgcgcgcat catcagcagg gccgccatga
     1741 acgagacgaa agtgccggcc gtgtcctggc gcagcatgtc ggtggagccg gccaggaaca
     1801 cgatcagcga gatggcgaat gcggcgatga actgcacgaa gccgccggcg cccgacgaac
     1861 tggccaccag cttcatgctc tgcacgcgga tgtagtcacc caccttcttg aagcgcgcca
     1921 cctcggccgc ctcgccgccg tagaccttga ccacgcggcg gccggtgatg gcctcatcgg
     1981 ccacgtgcgt cacgctgccc atcgactgct gcaggcgctg gttgatcatc ttgaaacgct
     2041 tgctcacgta gcgcaccgac caggcgacca ccggcaccag cgtgagcgtg atcagcgtca
     2101 gcttccagct gttgtacgac atgacgaaca acaggccaat gatggtcagg ctgtccttga
     2161 tcatcgaggt gatcacgctg ctcgccgact ccgacacctg gttgacgtgg taggtcagct
     2221 tgaccagcag gtcggcgttg cgcacgcggt cgtggtgcga caccggcagg tgcaggatct
     2281 ggtcgaacac ctcgccgcgc agctgcagca ccaccttctg gctgacccag gccatgccgt
     2341 acgacgacag gaacgcagcg gcgccgcggc acacgaacag gccgaggatg atccacggca
     2401 gcatctgccc ttcgcgcgcg ttcttcaggc cgaaggtgga atcggtcagc ggctgcacca
     2461 ggcgggcgaa gctcacgtcc acggaggcga acaccaccat gccgagcacg gccaccagga
     2521 acacgcgcca gtgcggcgcg gaatagccga gcagccggcg gtagaccgcg agcgcgccgg
     2581 gagtctggga atcggtcatg ccgcgaggtc ggcgggcggg ttcatgcgag gcgcgatgtt
     2641 agcatttgca cgccgctctc cgagggaaac accatggcca agacgctgtt cgagaagatc
     2701 atcgcgcgcg aaatcccggc aacgttcgtc cacgaggacg agcactgcgt ggccttccgc
     2761 gacatcagcc ccgccgcccc cacccacgtg ctgctggtgc cgcgcaagcc gctgaccatg
     2821 ctcaccgacg ccagcgccga ggaccagctg ctgctcggcc acctgatgct caccgcgtcg
     2881 aagatcgcgc gcgacctggg cgtgggcgac gccttccgcc tcgccgtgaa caacggtgcc
     2941 ggcgcaggcc agagcgtgtt ccacctgcac ctgcatattc tcggcgggcg gccgctgaag
     3001 tggccgccgg gctgaagctt cagcgcgcgc cggccgcgta cagcatggcg tagtggcgct
     3061 cggcgggcgg gcccttgagc tgcgtgggga tcagctgcag cgccgtcttg cctccggtgt
     3121 cgtaggcatt gatcttggcg gagtggtgga tcagcgcttc caggctgcgg tcgccgtagc
     3181 gcgacagcgc cgcgtacatc agcggcgtgc ggccttg
//
LOCUS       DASZZC010000729         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_880826,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000729 DASZZC010000000
VERSION     DASZZC010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880826"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHE37_15540"
     CDS             <1..355
                     /locus_tag="VHE37_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT37003.1"
                     /translation="DLHGPRAGAMWQMLCARAKDLRVEASNISADDRRGSAHWQAWYP
                     FSATGRRVHNIIDADFEFRDGLIVRHVDRFDFHRWAGQALGLAGKLLGGTSLLQNKVR
                     AQAAAGLDAWLQKNP"
     gene            complement(465..1010)
                     /locus_tag="VHE37_15545"
     CDS             complement(465..1010)
                     /locus_tag="VHE37_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HVT37004.1"
                     /translation="MTLCFIGAVACAAPTEKPDAELRARLVAAIRQADSFDDRFDAEV
                     WLLDMSTRLKPYVDDESRRLELLRLIHAESRRAKLAPELVLSVIQVESRFDRYAVSVS
                     GAQGLMQIMPFWLGEIGQPQDNLFDLRTNLRFGCTILKYYLEREKGSMVPALARYNGS
                     YGKPDYPYRVIEALNKRWYHS"
     gene            complement(1093..>2080)
                     /gene="proS"
                     /locus_tag="VHE37_15550"
     CDS             complement(1093..>2080)
                     /gene="proS"
                     /locus_tag="VHE37_15550"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HVT37005.1"
                     /translation="RPSGKPRASASAALTKVSTPGQKTCEQVSKFMNVPLTGKVKLLV
                     AHAREGGLIGIALRGDHQLNEFKASKHPKIKAPFTMATLDEVKAAFGCEVGYLGPVKC
                     PVPVIADFAAAEISDFVCGANENDHHLSGANWGRDCAEPETADLRNIVEGDPSPDGKG
                     TIRFFRGIEGGHVFQLGQKYSKSMKLSVLDEAGQAVTPQMGCYGIGVTRLVAAVIEQS
                     HDANGIIWPDAIAPFRVLIAPIGMDKSTAVKDAAEKLYADLTAAGVEVLLDDRGLRPG
                     GMFADADLIGVPHRIVIGDKGLAQGQFEYKHRRADKAQMIAAGAAAVLELLK"
BASE COUNT          338 a          696 c          665 g          381 t
ORIGIN      
        1 cgacctgcac gggccgcgcg ccggcgccat gtggcagatg ctgtgcgcgc gcgccaagga
       61 tctgcgcgtc gaggccagca acatctccgc ggacgaccgc cgcggcagcg cgcactggca
      121 agcgtggtac ccgttctccg ccaccgggcg gcgcgtgcac aacatcatcg acgccgactt
      181 cgaattccgc gacggcctga tcgtgcgcca cgtcgaccgc ttcgatttcc accgctgggc
      241 cgggcaggcg ctggggctcg cgggcaagct tctcggcggc acttcgctgc tgcagaacaa
      301 ggtacgggcg caggcggcgg cggggctcga cgcgtggctg cagaagaatc cgtgagcgga
      361 ttttttgctc caccgcaccg ccatttcgtg tgttattaat tattatctta atttacttta
      421 agactatatt taataacaat aaaaataccg gtgcggtgac agcttcagga gtggtaccag
      481 cgcttgttca gcgcctcgat cacgcggtac ggatagtccg gcttgccgta gctgccgttg
      541 tagcgcgcca gcgccggcac catcgaaccc ttctcgcgct cgaggtagta cttgaggatg
      601 gtgcaaccga agcgcaggtt ggtgcgcagg tcgaacaggt tgtcctgcgg ctggccgatt
      661 tcgccgagcc agaacggcat gatctgcatc agcccctgcg cgcctgaaac agacacggcg
      721 tagcggtcga accggctctc cacctggatc accgacagca ccagttccgg tgcgagcttg
      781 gcgcggcggc tctcggcgtg gatcagccgc agcagttcca ggcgccgcga ctcgtcgtcg
      841 acgtaaggct tgagccgcgt ggacatgtcg agcagccaga cctcggcatc gaaacggtcg
      901 tcgaagctgt cggcctgccg gatcgccgcg accaggcgcg cgcgcagttc ggcatcgggt
      961 ttttccgtgg gtgccgcaca ggcgacagca ccgatgaaac acaacgtcat ccccgcgaag
     1021 gctgggatcc agtttttcct tggcgccata tgaatcggca ctgggtcccc gcctgcgcgg
     1081 gaacgacagc ttctacttca gcagttccag caccgcggcc gcaccggcgg cgatcatctg
     1141 cgccttgtcg gcgcggcggt gcttgtactc gaactggccc tgcgccagcc ccttgtcgcc
     1201 gatgacgatg cggtgcggca cgccgatcag gtcggcgtcg gcgaacatgc cgccgggacg
     1261 caggccgcgg tcgtcgagca gcacttcgac gcccgccgcg gtgagatcgg cgtagagctt
     1321 ctccgccgcg tccttcaccg ccgtcgactt gtccatgcca atgggcgcga tgagtacgcg
     1381 gaacggcgct atggcgtccg gccagatgat gccgttggcg tcgtggctct gctcgatcac
     1441 cgcggcgacc aggcgcgtca caccgatgcc gtagcaaccc atctgcggcg tcaccgcctg
     1501 cccggcctcg tcgagcaccg acagcttcat gctcttcgaa tacttctggc cgagctggaa
     1561 cacgtggccg ccttcgatgc cgcggaagaa cctgatcgtg cccttgccgt ccggcgacgg
     1621 gtcgccctcg acaatgttgc gcaggtccgc ggtttcgggc tccgcgcagt cgcgccccca
     1681 gttggcaccg gacaggtgat ggtcgttttc attcgcgccg cagacgaagt cggagatttc
     1741 cgccgcggcg aaatcggcga tcaccggcac cgggcacttc accgggccga ggtaaccgac
     1801 ctcgcaaccg aatgcggctt tcacttcatc gagcgtggcc atggtgaacg gcgccttgat
     1861 cttcggatgc ttcgacgcct tgaactcgtt gagctggtga tcgccgcgca gcgcgatgcc
     1921 gatcagcccg ccctcgcgcg catgcgccac cagcagcttg accttgccgg tcagcggcac
     1981 gttcatgaac ttgctgacct gctcgcaggt tttctggccc ggcgtggaca ccttggtcag
     2041 cgccgcgctg gcggacgcgc gcggtttgcc cgacggccgc
//
LOCUS       DASZZC010000730         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_315727,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000730 DASZZC010000000
VERSION     DASZZC010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315727"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VHE37_15555"
     CDS             complement(<1..1092)
                     /locus_tag="VHE37_15555"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HVT37006.1"
                     /translation="MNKAWGILLLVAAMPAAQAADADKPNSYAAGLFNYNYPDASRDA
                     SYGLGASVLYGMPLVNDLDLEFNLSTYANKRESSNKHDYGYAGSFDLHYLLADSRQFG
                     LFFVGGVGGLYERQNNDNDLSPFADAGLAINFWNLLGNPALSLRTEARYYGVDHKAST
                     HRDHAAFAGASWLNDARFNAGLQWDFGAGKLRRGAAERDSDGDGVVDSKDECPNTPHG
                     VLVDRRGCPIEQDSDGDGVVDSKDACPNTPPGVVVDATGCPPAAPAPLPPPAPLLDSD
                     GDGVPDTIDDCPNTPAGMVVDVHGCPRDEDNDGVLNENDLCPHTPPGLKVDAQGCVIE
                     QTAVFHNINFEFGSEKLTAEAKTVLDGIAT"
     gene            complement(1254..>2167)
                     /locus_tag="VHE37_15560"
     CDS             complement(1254..>2167)
                     /locus_tag="VHE37_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HVT37007.1"
                     /translation="SAGGTLRAVDGIDFNLQAGETLGIVGESGCGKSTLARALVGLQP
                     VTSGAINFDGRNLAQLDSAAWRPLRREIQLVFQDPLASLDPRMTVGESIAEPLLALQP
                     ELDARARARQAAHWLERVGLSPAHINRYPHEFSGGQCQRIGIARALIVRPRLLICDEP
                     VSALDVSVQAQIVNLLADLREEYGLAMIFIAHDLAVVRHISHRVLVMYLGKAMEQAGR
                     DELFAAPAHPYTKALLASVPDADPRHGAVTRQVLEGEMPSAAHPPPGCVFATRCPMAD
                     EQCGRSAPHVRRSPSGSVVACHYVAAA"
BASE COUNT          319 a          742 c          753 g          353 t
ORIGIN      
        1 ggtagcgatg ccgtcgagca cggtcttggc ctcggcggtg agcttctccg agccgaactc
       61 gaagttgatg ttgtggaaca ccgcggtctg ctcgatcacg cagccctgcg cgtccacctt
      121 gagtcccggc ggcgtgtgcg ggcacagatc gttctcgttg agcacgccgt cgttgtcctc
      181 gtcacgcggg cagccgtgca catcgaccac catgccggcg ggcgtgttcg ggcagtcgtc
      241 gatggtgtcg ggcacgccgt caccgtcgct gtcgagcagc ggcgctggcg gcggcagcgg
      301 cgcgggcgct gccggcgggc agccggtggc atcgaccacc acgcccggcg gcgtgttcgg
      361 gcaggcgtcc ttgctgtcga ccacgccgtc gccgtcgctg tcctgctcga tcggacaacc
      421 gcggcggtcg accagcacgc catgcggcgt gttcgggcac tcgtccttgg aatcgaccac
      481 gccgtcgccg tcgctgtcgc gctcggccgc gccgcgcctc agtttgccgg cgccgaaatc
      541 ccactgcaga ccggcgttga agcgcgcatc gttgagccag ctggcgccgg caaaggccgc
      601 atggtcgcgg tgcgtgctgg ccttgtgatc gacgccgtag tagcgcgcct cggtgcgcag
      661 cgacagcgcc gggttgccca gcaggttcca gaaattgatg gccaggccgg cgtcggcgaa
      721 cggcgagagg tcgttgtcgt tgttctgccg ctcgtacagg ccacccacgc cgccgacgaa
      781 gaacaggccg aactgccgcg aatcggccag caggtagtgc aggtcaaaac tgccggcgta
      841 gccgtagtca tgcttgttgc tcgactcgcg cttgttggcg taggtggaga gattgaattc
      901 caggtcgaga tcattcacca gcggcatgcc gtacagcacg ctggcgccga gcccgtagga
      961 agcgtcgcgc gaggcatcgg ggtagttgta gttgaacagg cccgccgcgt agctgttggg
     1021 cttgtccgcg tcggcggcct gcgcagctgg catcgccgca accagcagca ggattcccca
     1081 ggctttgttc atgcgcattt tctcgctccc caaacacaca cgccggctta tttcgctttg
     1141 tgcctatatt acgcaaaact ccggtgcgca gccggcctgc gggaggtccg ccgatgcgtg
     1201 cgttccctgc tgcgattttc gtagcagcgc tgccgggccc gctcctgagc ggactacgcc
     1261 gccgccacgt agtggcaggc caccacgctg cctgagggcg agcggcgcac gtgcggcgcg
     1321 ctgcgcccgc actgctcgtc ggccatcgga cagcgcgtgg cgaacacgca gccaggcggc
     1381 ggatgcgcgg cgctgggcat ctcgccttcc agcacctgcc gcgtgacggc gccgtggcgc
     1441 ggatcggcat cgggcaccga ggccagcagc gccttggtgt acggatgcgc cggcgcggcg
     1501 aacagctcgt cgcggccggc ctgctccatc gccttgccca ggtacatcac cagcacgcgg
     1561 tggctgatgt ggcgcaccac cgcgaggtcg tgcgcaatga agatcattgc cagcccgtat
     1621 tcctcgcgca ggtcggccag caggttgacg atctgcgcct gcaccgagac gtcgagcgcc
     1681 gaaaccggct cgtcgcagat cagcaggcgc ggacgcacga tcagcgcacg cgcgatgccg
     1741 atgcgctggc actggccgcc ggaaaattca tgcggatagc ggttgatgtg cgcaggcgac
     1801 agccccaccc gctcgagcca gtgcgcggcc tgccgcgcgc gggcgcgcgc atcgagttcc
     1861 ggctgcaggg ccagcagcgg ctcggcgatc gattcgccga ccgtcatgcg cgggtcgagg
     1921 ctggccagcg ggtcctggaa caccagctgg atctcgcggc gcagcgggcg ccaggcggcg
     1981 ctgtcgagct gcgccaggtt gcgaccgtcg aagttaatgg cgcccgacgt caccggttgc
     2041 aggccgacca gcgcgcgcgc cagcgtggac ttgccgcagc ccgactcgcc gacgatgcca
     2101 agcgtctcgc cagcctgcag gttgaaatcg atgccgtcca ccgcgcgcaa ggtgccgccg
     2161 gcgctcc
//
LOCUS       DASZZC010000731         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_880942,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000731 DASZZC010000000
VERSION     DASZZC010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880942"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..285
                     /locus_tag="VHE37_15565"
                     /pseudo
     CDS             1..285
                     /locus_tag="VHE37_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145411.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            275..475
                     /gene="rpmD"
                     /locus_tag="VHE37_15570"
     CDS             275..475
                     /gene="rpmD"
                     /locus_tag="VHE37_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HVT37008.1"
                     /translation="MAAKKQTTTGTVKVRLVGGLRGVQSRHRLSVKALGLNRTGDVRE
                     LKDSPQVRGLINTVNYLVRVEE"
     gene            479..910
                     /gene="rplO"
                     /locus_tag="VHE37_15575"
     CDS             479..910
                     /gene="rplO"
                     /locus_tag="VHE37_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HVT37009.1"
                     /translation="MRLNTLKPGKGARKERRRVARGIGSGLGKTAGRGHKGQHARAGN
                     THTVGFEGGQMPLQRRLPKVGFRSKKLAESQQVFLYQLAGLKKDSIDLAALREAKLVD
                     GRAKRVKIVKKGEIDRAVKVSGILATAGAKAAIEAAGGSVE"
     gene            946..2247
                     /gene="secY"
                     /locus_tag="VHE37_15580"
     CDS             946..2247
                     /gene="secY"
                     /locus_tag="VHE37_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVT37010.1"
                     /translation="MSQFGKLTELRQRLLFLVGALVVFRLGSFIPVPGVNPEAMTRMI
                     EQGGGLLNMFNMFSGGALARFSLFALGVIPYISASIVVQMMGSVLPAWQQLKKEGQAG
                     QRIMTKYTRWGTVGLSAFQAFGIAMALQKEGISGGVPVVYDPGFGFIFASVIGLTAGS
                     MFLMWLGEQMTERGIGNGISLIIFAGIVAGLPQALTQTFTMASNGQLSAIKVLIVVVI
                     VLGVTAFCVFMESAQRRITVNYARRQGGANAYQNQSSHLPLKINMSGVIPPIFASSLI
                     LFPATASTWFSNASQMHWLQRLTTALTPGEPPYMILYAAMIIIFAFFYTAIVFNSQET
                     ADNLKRSGALIPGLRPGKATADYIDAVLTRLTGAGAVYLAAVCLIPTFMQTEWHVPFY
                     FGGTSLLIVVVVAMDFRAQVSAHLVSHQYDSLLKKANLRRK"
     gene            2372..>2450
                     /locus_tag="VHE37_15585"
     CDS             2372..>2450
                     /locus_tag="VHE37_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVT37011.1"
                     /translation="MARIAGVNLPVNKHVCIGLQSIYGIG"
BASE COUNT          424 a          770 c          827 g          429 t
ORIGIN      
        1 cggcttgaac aacggcacca tctggtacgc ggtcaaggcc aaccacggcg ccgcgcgcgt
       61 gttcatgcag ccggcttccg aaggtaccgg cgtgatcgcc ggcggcgcga tgcgcgccgt
      121 gctggaagtg gtcggcgtga aggacgtgct ggccaaggcg gtcggctcgc gcaatccgat
      181 caacctggtg cgcgccacca tcaagggcct gcagtccgtc gcgagcccgg aattcatcgc
      241 ggccaagcgt ggcagaaggt cgaggagctg ggtcatggcg gctaagaaac aaaccaccac
      301 cggcaccgtg aaggtgcgcc tcgtgggcgg cctgcgcggc gtgcagtcgc ggcaccgcct
      361 gagcgtcaaa gcgctgggcc tgaaccgcac cggcgacgtg cgcgaattga aggacagccc
      421 gcaggtacgc ggcctgatca acacggtcaa ctatctggtc cgggtcgagg agtaagtcat
      481 gcgtttgaat acattgaaac ccggcaaggg cgcacgcaag gaacgccgcc gcgtcgcgcg
      541 cggcattggt tccggcctcg gcaagaccgc gggccgcggc cacaagggcc agcacgcgcg
      601 cgcgggcaac acgcataccg tcggcttcga aggcggccag atgccgttgc agcgccgcct
      661 gcccaaggtc ggtttccgct ccaagaagct ggccgagtcg cagcaggtgt tcctgtacca
      721 gctcgcgggc ctgaagaagg attcgatcga cttggccgcg ttgcgtgaag ccaagttggt
      781 cgacggccgc gccaagcgcg tcaagatcgt caagaagggc gagatcgacc gtgcggtgaa
      841 ggtatccggc atcctggcaa ccgcaggcgc caaggcggcg atcgaagccg ccggcggctc
      901 ggtggagtaa gcatcttggc cgcaggcaag acatcgtcca atccgctgtc ccagttcggc
      961 aagttgaccg aactgcgcca gcgcctgctg ttcctggtcg gcgcgctggt cgtgttccgg
     1021 ctgggttcgt tcatccccgt ccccggcgtc aacccggaag cgatgacgcg gatgatcgag
     1081 cagggcggcg gactcctgaa catgttcaac atgttctcgg gcggcgcgct ggcgcgcttc
     1141 tcgctgttcg cgctgggcgt gatcccgtac atctccgcat cgatcgtggt gcagatgatg
     1201 ggttcggtgc tgccggcgtg gcagcagctc aagaaggaag gccaggccgg ccagcgcatc
     1261 atgaccaagt acacccgctg gggtacggtc gggctgtcgg cgttccaggc cttcggcatc
     1321 gcgatggcgc tgcagaagga aggcatctcc ggcggcgtgc cggtggtgta cgaccccggc
     1381 ttcggcttca tcttcgcatc ggtgatcggc ctcactgccg gcagcatgtt cctgatgtgg
     1441 ctgggcgagc agatgaccga gcgcggcatc ggcaacggca tctcgctgat catcttcgcg
     1501 ggcatcgtgg cgggcctgcc gcaggcgctg acgcagacct tcacgatggc cagcaacggc
     1561 cagctgtccg cgatcaaggt gttgatcgtg gtggtgatcg tgctgggcgt gaccgcgttc
     1621 tgcgtgttca tggaaagcgc gcagcggcgg atcacggtga actacgcacg tcggcagggc
     1681 ggcgccaacg cgtaccagaa ccagagctcg cacctgccgc tgaagatcaa catgtcgggc
     1741 gtgatcccgc cgatcttcgc gtcgtccttg atcctgttcc cggcgaccgc atctacatgg
     1801 ttctcgaacg cgagccagat gcactggttg cagcgcctga ccaccgcgct gacgccgggc
     1861 gagccgccgt acatgatcct gtacgcggcg atgatcatca tcttcgcgtt cttctacacc
     1921 gcgatcgtgt tcaactctca ggaaaccgcc gacaacctga agcgttcggg cgcgctgatc
     1981 ccgggcctgc gcccgggcaa ggcgacggcc gattacatcg acgccgtgct gacccgtttg
     2041 acgggggcgg gcgcggtgta tctggccgcg gtctgcctga tcccgacgtt catgcagacc
     2101 gagtggcacg tgccgttcta cttcggtggc acttcgctcc tgatcgtggt ggtggtggcg
     2161 atggacttcc gcgcgcaggt ttccgcgcac ctggtctcgc accagtacga cagtttgttg
     2221 aagaaggcca atttgcgccg caagtagccc gcgccggctg gccggcgggg gtggcagtgc
     2281 cgggtggggc ctacccccca ttcggatgaa tttcaagcta taattgacag tttgccgaat
     2341 cggcaacgca ttcgaggtgg agtaaccgca catggcacgt atcgcgggcg tcaacctgcc
     2401 ggtcaacaag cacgtttgca tcggcctgca gagcatctac ggcatcggcc
//
LOCUS       DASZZC010000732         5144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_255559,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000732 DASZZC010000000
VERSION     DASZZC010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255559"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VHE37_15590"
     CDS             <1..1338
                     /locus_tag="VHE37_15590"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT37012.1"
                     /translation="SLAYNFFLLPPLYTFTIGDPDNVLAFFAFIIVALIISQLTSRTR
                     AQTLAAREQAATTAALLNFSRRLAGTRKLEQLYDTAAQEVAQLLKVECVVLAPAGEEL
                     KLVAAVPATTKIEEADIAAATWCWRKTQPTGWGSDTLPGAHRLYVPMTGGTATLGVIG
                     VSRIEKFSGSAPLHAHERRLLDALADLTVIALDRILLARDVDQSKMLAQTEKLRSALL
                     TSISHDLRTPLSSILGAISSLRAYGKKYDEKARDELLTTAQDETERMSRFVANLLDMT
                     RLDSGALAPKAEACDLNDVAGAALRRAEKLLAKHQVQIDIPGNVPMVKLDFVLLEQVL
                     VNLLDNAAKYSPAGSTVELAVNAHRYAVTLEVRDRGPGIPPEDLQRIFDPFFRVRQGD
                     QQRAGTGLGLAVCRGFVQAMNARISASNRAGGGAVFTIEFPASLLVRADGVPA"
     gene            1335..2018
                     /locus_tag="VHE37_15595"
     CDS             1335..2018
                     /locus_tag="VHE37_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT37013.1"
                     /translation="MSEGARVLVVDDEPAIRRLLRTTLAAQGYEVLEAADAAGALAQA
                     RGRIDAIILDLGLPDRDGLAVIAELRRDSAVPILVLSSRDQERGKVEALDAGADDYIT
                     KPFGAEELLARIRAALRHRLQQQGTVPVFESSALRVDLVRRLVTLRGAELKLSPKEYD
                     ILAQLVLHAGKVLTHRHLLEKIWGSAATTDVQYLRVYMRQLRQKIEDDPNQPKLIITE
                     PGVGYRLRA"
     gene            2230..2994
                     /locus_tag="VHE37_15600"
     CDS             2230..2994
                     /locus_tag="VHE37_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HVT37014.1"
                     /translation="MKRVVFNQKGGVGKTTIVCNLAAIAAARGLRTLVIDLDTQGNAT
                     QYLMGGSPVRADRTVADFFEGTLSFSIAPPAFVTFASNTPFENLDVVAASPRLDELIV
                     KLEAKQKIYKLRDALSKLRGYDAVFIDTPPALNFYSRSALIAADRVLIPFDCDEFARQ
                     ALYTLLDNIQEIKGDHNEGLEVEGIIANQYQSRSKLPARLVEELKAEGRPVLEQTIGS
                     SVIVRQSHETHRPLVNYDPGHRVTKEYVALYDALAA"
     gene            complement(3054..4727)
                     /locus_tag="VHE37_15605"
     CDS             complement(3054..4727)
                     /locus_tag="VHE37_15605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HVT37015.1"
                     /translation="MEKNKDVLARTRISLAVCALAAACASGAAQAAWLGTATGALPLD
                     ASVLRGELSSGESVRVAVSLAPRNAAELASFIGELNRAGSPAYGHFLTSQQYLERYAP
                     TAAQVRAVVAHLQKSGFVNIDVAPNRLLVTADGTAGVVQRAFNTVLQRVDLGGMSAYA
                     NTRPAQVPQALAGIVTAVLGLQTVEKLHPMNIVAARVNPQATAAAHGFNPTAFAALYG
                     ASALPTASTTNVGIISDGVVTQTITDLRQFESQNSLPQVPVSVVQVGAAGTDTSGTVE
                     WDLDSQDIQSMAGGTVQQLLFYDTTSLANADITAAFNKAVSDNTAKVVNVSLGECETS
                     AQTDGMVAADDAIFEAGVAQGQIFSISTGDSGSHECSVLFATNNNTKQSYPAVSPYVM
                     AIGGTSLLTTTAGAWASETVWSGAGGGPSTVENKPSWQTGVSGTKRGVPDVSFDADPN
                     TGAIIIVNGSSEQVGGTSLAAPLFAGLWARIESANSNALGFPDASFYHYLPTQPTLHH
                     DVTSGSNGSGGLLGGGSNYTAAAGWDFASGFGSLVGGNLATFVSQTPGF"
     gene            complement(4943..>5144)
                     /locus_tag="VHE37_15610"
     CDS             complement(4943..>5144)
                     /locus_tag="VHE37_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37016.1"
                     /translation="NNALGYPNPSFYKYLPGHATLHHDVTSGNNGSGGAYNAAAGWDY
                     TTGFGSLIGAPLATFISQTPGF"
BASE COUNT          872 a         1740 c         1699 g          833 t
ORIGIN      
        1 tcgctggcct acaacttctt cctgctgccg ccgctgtaca ccttcaccat cggcgatccg
       61 gacaacgtgc tggcgttctt cgccttcatc atcgtggcgc tgatcatcag ccagctcacc
      121 tcgcgcacgc gcgcgcagac gctggccgcg cgcgagcagg cggccaccac cgccgcgctg
      181 ctcaacttca gccggcgcct ggccggcacg cgcaagctcg agcagctgta tgacacggcg
      241 gcgcaggaag tggcccagct gctgaaggtg gaatgcgtgg tgctggcacc cgccggcgaa
      301 gagctcaagc tggtggctgc agtgccggcc accacgaaaa tcgaagaagc cgacatcgcc
      361 gccgccacct ggtgctggcg caagacgcag cccaccggct ggggctccga cacgctgccg
      421 ggcgcgcatc gcctgtacgt gccgatgacg ggcggcaccg ccacgctggg cgtgatcggc
      481 gtgtcgcgca tcgagaaatt ctccggctcg gcgccactgc acgcacacga gcggcgcctg
      541 ctcgacgcgc tggcggatct caccgtcatc gcgctcgacc gcatcctgct ggcgcgcgat
      601 gtcgaccaga gcaagatgct ggcgcagacc gagaagctgc gctcggcgct gctcacttcc
      661 atctcgcacg acctgcgcac gccgctgtcc agcatcctgg gcgccatcag cagcctgcgc
      721 gcatacggca agaaatacga cgagaaggcg cgcgacgaac tactcaccac cgcgcaggac
      781 gagaccgagc gcatgagccg cttcgtcgcc aacctgctgg acatgacgcg gctggattcc
      841 ggcgcgctgg cgcccaaggc cgaggcctgc gacctcaacg acgtcgccgg cgccgcgctg
      901 cgccgagcgg agaaattgct ggcaaagcac caggtgcaga tcgacatccc cggcaacgtg
      961 ccgatggtga agctggactt cgtgctgctt gagcaagtgc tggtcaacct gctcgacaat
     1021 gcggcgaagt actcgcccgc cggcagcacg gtggaacttg cggtgaacgc tcatcgctac
     1081 gcggtcacgc tggaagtgcg cgaccgcggc cccggcattc cgccggaaga cctgcagcgc
     1141 atcttcgatc cgttcttccg cgtgcgccag ggcgaccagc agcgcgccgg cactggcctg
     1201 gggctcgcgg tgtgccgcgg cttcgtgcag gcgatgaatg cgcgcatcag cgcgtccaac
     1261 cgcgcgggtg gcggcgcggt gttcaccatc gagtttccgg cgagcctgct ggtgcgcgcg
     1321 gacggcgtgc ccgcatgagc gaaggcgcgc gcgtgctggt ggtggacgac gagccggcca
     1381 tccgccggct gctgcgcacc acgctggcgg cgcagggtta cgaagtgctg gaagcggccg
     1441 atgccgctgg cgcgctggcg caggcgcgcg gcaggatcga cgccatcatc ctcgatctgg
     1501 gcctgcccga ccgcgatggc ctcgcggtga tcgccgagct gcgccgcgat tccgcggtgc
     1561 cgatcctggt gctgtccagc cgcgaccagg agcgcggcaa ggtggaagcg ctcgacgccg
     1621 gcgccgacga ctacatcacc aagccgttcg gcgcggagga actgctggcg cgcatccgcg
     1681 ccgcgctgcg ccaccgcctg cagcaacagg gcacagtgcc ggtgttcgaa agcagcgcgc
     1741 tgcgcgtcga cctggtgcgc cggctggtca cgctgcgcgg cgccgagctg aagctgtcgc
     1801 ccaaggaata cgacatcctg gcgcagctgg tgctgcacgc gggcaaggtg ctcacgcacc
     1861 ggcacctgct ggagaagatc tggggctcgg cggccaccac cgacgtgcag tacctgcgcg
     1921 tgtacatgcg ccagctgcgg cagaaaatcg aggacgatcc caaccagccg aagctgatca
     1981 tcaccgagcc gggcgtgggc taccggctgc gcgcctgagc ggcccgggca cccataccgc
     2041 actgcctttt ttggtgttat taattgttta atttttttaa aaacataata taattaataa
     2101 catcaaaaaa gccggtgcgg tacacatgaa attccgcgtt tgaaggctac aatcgcgcgc
     2161 cgcgaacgcg gccgttcagc ggcatggaag ctgcgagcga catgcggttc gacgaggaag
     2221 acggcgatga tcaagcgcgt ggtgttcaac cagaaaggcg gcgtgggcaa gaccaccatc
     2281 gtgtgcaacc tggcggcgat cgcggcggcg cgcggactgc gcacgctggt gatcgacctc
     2341 gacacgcagg gcaatgccac gcagtacctg atgggcggct cgccggtgcg cgccgaccgc
     2401 acggtggccg atttcttcga gggcacgctg tcgttctcca tcgccccgcc cgccttcgtc
     2461 acctttgcca gcaacacgcc gtttgaaaat cttgacgtgg tggcggccag cccgcggctc
     2521 gacgagctga tcgtcaagct cgaggccaag cagaagatct acaagctgcg cgatgcgctt
     2581 tccaagctgc gcggctacga cgcggtgttc atcgacacac cgccggcgct gaatttctac
     2641 tcgcgcagcg cgctgatcgc cgccgaccgc gtgctgatcc cgttcgactg cgacgagttc
     2701 gcgcgccagg cgctgtacac gctgctggac aacatccagg aaatcaaagg cgaccacaac
     2761 gaggggctcg aagtggaagg catcatcgcc aaccagtacc agagccgcag caagctgcct
     2821 gcgcgcctgg tcgaggaact caaggccgaa ggccggccgg tgctcgagca gaccatcggc
     2881 agttcggtga tcgtgcgcca gtcgcacgag acgcaccggc cgctggtcaa ctacgatccg
     2941 gggcaccgcg tgaccaagga atacgtcgcg ctgtacgacg cgctcgccgc gtaagtgctt
     3001 cgagcatgaa aaaggccgcg cggtgcgcgg cctttttttg tgcccgtgaa acttcagaag
     3061 ccgggcgtct ggctgacgaa cgttgccagg ttgccgccaa ccaggctgcc gaagcccgag
     3121 gcgaaatccc aacccgcggc cgcggtgtag ttgctgccgc cgccgagcag gccgccgctg
     3181 ccgttgctgc cgctggtcac gtcgtgatgc agcgtgggct gcgtcggcag gtagtgatag
     3241 aagctggcat cgggaaatcc cagcgcattg ctgttggcgc tctcaatgcg cgcccacagc
     3301 ccggcgaaca gcggcgccgc caggctggtg ccgcccacct gttccgagct gccgttgacg
     3361 atgatgatcg cgccggtatt cggatcggcg tcgaacgaca cgtcgggcac accgcgcttg
     3421 gtgccggaca cgccggtctg ccagctcggc ttgttctcga cggtgctcgg cccgccgcca
     3481 gcgccgctcc acaccgtctc gctggcccag gcgccggcag tggtggtcag cagcgaggtg
     3541 ccaccgattg ccatgacgta gggcgacacc gccgggtagc tctgcttcgt gttgttgttg
     3601 gtcgcgaaca gtaccgagca ttcatgcgaa cccgagtcgc cggtggaaat cgagaagatc
     3661 tggccctgcg ccacgccggc ctcgaagatg gcgtcgtcag cggccaccat gccgtcggtc
     3721 tgcgcggacg tctcgcactc gcccagtgac acgttgacca ccttggcagt gttgtcgctc
     3781 accgccttgt tgaacgcggc ggtgatgtcc gcgttcgcca gggaggtggt gtcatagaac
     3841 agcagctgct gcacggttcc gccggccatg gactggatgt cctggctgtc gagatcccac
     3901 tccaccgtgc cggaggtgtc ggtccccgcc gcgcccacct gcaccacgct caccggaacc
     3961 tgcggcagcg aattctggct ctcgaactgg cgcaggtcgg tgatggtctg cgtcaccacg
     4021 ccgtcgctga tgatgccgac gttggtggtt gacgcggtcg gcagcgcact ggcgccatac
     4081 aacgcggcga acgcggtggg attgaagcca tgcgcagccg ccgtcgcctg cgggttgacc
     4141 cgtgccgcga cgatgttcat cgggtgcagc ttctccaccg tctgcaatcc gagcaccgcg
     4201 gtgacaatgc ccgccagcgc ctgcggcacc tgcgccgggc gcgtgttggc gtacgcgctc
     4261 atgccgccca gatcgacccg ctgcagcacc gtgttgaacg cgcgctgcac gacaccggcg
     4321 gtgccatctg ccgtcaccag caggcggttg ggtgcgacat cgatgttgac gaagccggat
     4381 ttctgcaggt gcgccaccac cgcccgcacc tgcgccgcgg tcggcgcgta gcgctcgaga
     4441 tattgctgcg aggtgaggaa atgcccgtac gccgggctgc cggcgcgatt cagttcacca
     4501 atgaaactcg cgagttccgc agcgttgcgc ggcgccaggc tcaccgccac gcgcacgctc
     4561 tcaccggaac tgagttcgcc gcgcagcacg ctggcatcca gcggcaatgc gccggtggca
     4621 gtgccgagcc atgccgcctg cgccgcgccc gaagcgcagg ctgccgccag cgcgcacacc
     4681 gccagtgaaa tgcgcgtgcg cgcgagaaca tccttgttct tctccatcgt ctgctcctcg
     4741 tgatgcccgc ggcgcgaatc cgttcgcatt cggggctgat ggcgggcgat cctagcgaag
     4801 aaccttcagc cgcaatacgg cgcagcgctg actcaacggc gcggaaaagt cctaccgaac
     4861 tttcgcggtg cccgcgccgt ccagtttcga cgtgccatga aaaaggccgc ctgcgggcgg
     4921 ccttttccgt tgcgccggaa aatcagaagc ccggcgtctg gctgatgaac gtggccagcg
     4981 gcgcgccgat caggctgccg aagccggtgg tgtagtccca gcccgccgcg gcgttatatg
     5041 cgccgccgct gccgttgttg ccgctggtca cgtcgtgatg cagcgtcgcg tggcccggca
     5101 ggtacttgta gaacgatggg ttgggataac ccagcgcatt gttg
//
LOCUS       DASZZC010000733         9130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_693625,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000733 DASZZC010000000
VERSION     DASZZC010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693625"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1993
                     /locus_tag="VHE37_15615"
     CDS             368..1993
                     /locus_tag="VHE37_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVT37017.1"
                     /translation="MPVIESKVDTSSSQFQANREEMLKYIEQFRAIEAKGRAEEESKR
                     ERFHKKGQLLPRERVHLMLDRGSPWLELSTLAGYKHHDDKDGSLAGGNNISGVGYVSG
                     VRCMVNASNFAIKGGTMTPWGVQKGLRIQEIVLQQKLPSVTMIESGGANLLYQAELFI
                     PGGTTFANQCRISAAGIPQVCVVHGSSTAGGAYMPGLSDYVVMVRKQAKVFLAGPPLL
                     RAATGEIAKDEDLGGAEMHTQVAGTAEFLAENDADGIRIARDIVKNLNWNAQRPKLDL
                     LEVRAPLYDIEELCGVVPPDHRKPYDCREVIARLVDGSEFLDFKTEYDQQTICGRAVI
                     HGHRVGIISNNGPITTKGATKAGQFIQLCCQAGIPIIYLMNTTGYMVGSESEQGGIVK
                     HGSKMIQAVANATVPQITIVMGGSFGAGNYGMCGRGFGPHFIFAWPNSRTSVMGGEQA
                     AKVMSIITRDKWLKEGRQISEADEKQLAAIESNIVKQFDRESNAFAVTARLYDDGLID
                     PRDTRKVLGMCLSVCREGRARQLFPNSFGVARL"
     gene            1993..2346
                     /locus_tag="VHE37_15620"
     CDS             1993..2346
                     /locus_tag="VHE37_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37018.1"
                     /translation="MPVAAINRFFDANAEAIEHLYTFFRNIGAMITLTVPGGMLAFYP
                     EKFSMQHSELMAVLGEALILFAGSMSVFATLHYWRRMEASGTDHRVLFKSISAAAGFS
                     YPLFAVIAAIEHIGR"
     gene            2350..3516
                     /locus_tag="VHE37_15625"
     CDS             2350..3516
                     /locus_tag="VHE37_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT37019.1"
                     /translation="MQFTAEHQSFYDTTRKFVEEEINPHVAEWEASGLFPARQVFKKM
                     GDLGLLGISKPESVGGLGLDYSYQIMFSEALGNCTCGGIPMAIGVQTDMATPALARFG
                     SDYLKENFLKPAVAGDMVACIAVSEPGAGSDVAGVKTTARKVGGDYVISGSKMWITNG
                     TQGDFACLLANTGEPVDGGRHGNKSLIVVPLDAKGIDRKTKLDKLGQRSSDTAMIFLD
                     EVKVPAKNLIGEEGKGFVYQMMQFQEERLFLSASILRAMEICVEQTATYTRQRQAFGQ
                     SVLDNQYVHFRLSELATEIEACRSLLYRATEELVRGGDVTLLASMTKLKTARVAREVA
                     DGCLQYWGGQGFMWENQVSQFYRDLRLHSIGGGADEVMLSIISKRLGLGGKKKK"
     gene            3513..4322
                     /locus_tag="VHE37_15630"
     CDS             3513..4322
                     /locus_tag="VHE37_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVT37020.1"
                     /translation="MNLPESKSLLLRAEGGVLHLTLNRPESRNAMSPEMLAEIEAAFD
                     SIAGSSEIRIVVLRGAGGHFCAGADLKGMMGAGMKPPAPGQPDPLAAINRAFGTMLRK
                     VEAAPQVVIAVCEGGVLGGGFGLACVSDIALAHTEAKFGMPETSRGLPPAQIAPFVVE
                     RIGLTQARRLALTSAQFRGEEALKLGLVHEVFGNEEELNTKLGAYIKQVLNCAPQANA
                     LTKQIMLQAGKVPMDQVLDDAAAKFAACARGSEAPEGIAAFMQKRAPKWAQ"
     gene            4336..6324
                     /locus_tag="VHE37_15635"
     CDS             4336..6324
                     /locus_tag="VHE37_15635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HVT37021.1"
                     /translation="MKKFDKVLVANRGEIAVRVIRGAKKLGYRTVAVYSDADKDSLHV
                     READEAVRIGPPAAKESYLVIDNILAAAKQSGAQAIHPGYGFLSERAEFSTAVHDAGL
                     TFIGPDAFSIDAMGNKSASKIRMIAAGVPCVPGYQGDDQADETLRKEAVKAGLPVMIK
                     AAAGGGGRGMRLVHDEKELLANIQSARSEATNAFGSGQLLIEKAVMDARHVEIQVFGD
                     RHGNVVHFGERDCSVQRRNQKVVEEAPSPAVDDALRLKMGAAAVAAAKAVNYVGAGTV
                     EFMLARDGQFYFLEMNTRLQVEHPVTELVYGVDLVEWQLRVAQGEKLPLTQEEILKRR
                     NGWAIEVRLCAEDPRDNYMPQAGPVLAWRAPQGAGVRVDTYLESGCRISPYYDSMQAK
                     IIAYGDTRESARQRLLKALDETVLLGVINNKDYLHAILSHEAFAGGDFSTAFIGKYFP
                     ADAVARSAPTHRHIALAAVALYMDDAHRLAAEHGLTQEFVGWHSSHETPADFKVKWRD
                     RAYNLEVLVHDGHLFTTTVEGEKIEIDVTRSSEHDFQYLCAGVQATARIAHDGDMVWI
                     SAEGATHGYTDVTLAPVQGAAAGSDGRLLANSDGKVVAVHVKPGDVIAKGQTLAVLEA
                     MKMEFQLALPVGGTVEAVSVSAGQQVKNRQLLVQVKPA"
     gene            complement(6781..8616)
                     /locus_tag="VHE37_15640"
     CDS             complement(6781..8616)
                     /locus_tag="VHE37_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HVT37022.1"
                     /translation="MRKNMWGKTIVFSAAAALAALATAPAQADQATFDPGWYINLNGG
                     FNSLSKADNTVSNSTSTPSTTSLNILGLIAIPAGTPLDPLLNPLAGALCPTPLGALLC
                     ATGGSTTTVTGTSQTVKFKGGSTFGLGFGYQAANGLRPEFELSYRNNDIESAVTNVTT
                     TTTTGGVTSAPTTTSITATNVAGKAQSYGAIGNLWYDFAPTAGVDPYIGAGLGATKVQ
                     YKDFSGSNAGTAFGPINKSSTVFSWQLGAGVNFKVTDHTRLSIDYRYLAANNVAFDFG
                     GGTELDTSYKNNSIMLGMHYAFGSWSGKNPKCPNAPKGVPMDAEGCPLDSDGDGVPDY
                     MDKCPNTPAGVKVDANGCELDSDGDGVPDSRDQCPNTPKGVAVDTNGCPLDSDGDGVP
                     DYLDKCPNTPKGTVVDANGCPALDSDGDGVPDYLDKCPNTPKGVQVDANGCPLDSDGD
                     GIPDYLDECPHTPAGAKILPNGCAPVGDCRKPRPGEEVDANGCALEKTFILRGVKFEF
                     DSDRLTQDAKKILDGVATTLNNYPEINVELGGHTDNIGTDAYNLGLSERRSNSVKNYL
                     TGKGVAAARMNPVGYGETQPIATNDTEEGREENRRVELKVIEQTQ"
     gene            complement(8835..>9130)
                     /locus_tag="VHE37_15645"
     CDS             complement(8835..>9130)
                     /locus_tag="VHE37_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT37023.1"
                     /translation="HQLVDMKMRNEAVKANLDHLAWRIGQKHMPVAEVCMLKNLATTS
                     LEWVANEAMQIFGGAGYLRGAKVERIYRETKVLSIGGGSIEIMKDLAARQLGY"
BASE COUNT         1650 a         2834 c         3090 g         1556 t
ORIGIN      
        1 gaagaaggcc atcatgtcgc gcagcgcgcg cgtgctgatg gaagggcacg tgcgcgtgcc
       61 gggcgatgcg ttctgatttc gcggatcgcc tcgcggctga ttcggcgcgt gggagtgtct
      121 accgcaccgg ccctcggtgt gttatataaa gttgatcaaa acgaaaaata ataaataaaa
      181 tgtaacatcg tcatgccgga tgcggtggcg atttcttcag tgctcactgc tccgggttga
      241 tgcggggagc gcgcagcggg acgcaagggt ttttctccag ggccggcagt tgccgcgcgc
      301 catgcatttc cgcataaaat acgcgccgct ggtgagtctg actcaccaaa agaatccgga
      361 gaggcgcatg cccgtcatcg agagcaaggt cgacacgtcc agcagccagt tccaggccaa
      421 ccgcgaggag atgctcaagt acatcgagca gttccgcgcc atcgaggcca agggccgcgc
      481 cgaggaagag agcaagcgcg agcgcttcca caagaagggc cagctgctgc cgcgcgagcg
      541 cgtgcatttg atgctggatc gcggctcgcc gtggctggag ctttcgacgc tcgcgggcta
      601 caagcaccac gacgacaagg atggctcgct ggccggcggc aacaacatca gcggcgtcgg
      661 ctacgtgagc ggcgtgcgct gcatggtcaa cgccagcaac ttcgccatca agggcggcac
      721 catgacgccg tggggcgtgc agaagggcct gcgcatccag gaaatcgtgc tgcagcagaa
      781 gctgccgtcg gtgaccatga tcgaatcggg cggcgccaat cttctctacc aggcggagct
      841 gttcattccc ggcggcacca ccttcgccaa ccagtgccgc atctccgcgg ccggcatccc
      901 gcaggtgtgc gtggtgcatg gctcgtccac cgcgggcggc gcctacatgc cgggcctgtc
      961 cgactacgtg gtgatggtgc gcaagcaggc gaaggtgttc ctcgcaggcc cgccgctgct
     1021 gcgcgcggcc accggcgaga tcgccaagga tgaagatctc ggcggcgccg agatgcacac
     1081 gcaggtggcc ggcactgcgg aattcctggc ggagaacgac gccgacggca tccgcattgc
     1141 gcgcgacatc gtgaagaacc tgaactggaa tgcgcagcgg ccgaaactgg acttgcttga
     1201 agtgcgcgcg ccgctgtacg acatcgagga gctgtgcggc gtggtgccgc cggaccaccg
     1261 caagccgtac gactgccgcg aggtgatcgc gcgccttgtc gacggcagcg agttcctcga
     1321 cttcaagacc gagtatgacc agcagaccat ctgcggccgc gcggtgatcc acggccatcg
     1381 cgtcggcatc atcagcaaca acggcccgat tactaccaaa ggcgcgacca aggccggcca
     1441 gttcatccag ctgtgctgcc aggccggcat tcccatcatt tatttgatga acaccaccgg
     1501 gtacatggtc ggcagcgaga gcgagcaggg cggcatcgtc aagcacggca gcaagatgat
     1561 ccaggcggtg gccaacgcca cggtgccgca gatcaccatc gtcatgggcg gctcgttcgg
     1621 cgccggcaac tacggcatgt gcggacgcgg cttcggcccg cacttcatct ttgcctggcc
     1681 caactcgcgc acctcggtga tgggcggcga gcaggcggcc aaggtgatga gcatcatcac
     1741 gcgcgacaag tggctgaagg aaggcaggca gatttccgag gccgacgaga agcagctggc
     1801 ggccatcgag agcaacatcg tcaagcagtt cgaccgcgag tcgaacgcat ttgccgtgac
     1861 tgcgcgcctc tacgacgatg gcctgatcga tccgcgcgac acgcgcaagg tgctgggcat
     1921 gtgcctgtcg gtgtgccgcg aaggccgcgc gcgccagctg ttccccaaca gcttcggtgt
     1981 ggcgcgcctg taatgccggt cgcagcgatc aatcgcttct tcgatgccaa tgcggaggcc
     2041 atcgagcacc tgtacacgtt tttccgcaac atcggcgcca tgatcacgct gaccgtgccc
     2101 ggcggcatgc tggcgttcta cccggaaaaa ttctccatgc agcactcgga gctgatggcg
     2161 gtgctgggtg aagcgctgat cctgtttgcc ggatcgatgt cggtgttcgc cacgctgcat
     2221 tactggcgcc gcatggaagc cagcggcacc gatcaccgcg tcctgttcaa gtccatttcc
     2281 gcggcggcgg gcttctccta cccgctgttc gccgtcatcg ccgccatcga gcacattgga
     2341 aggtaagtca tgcaattcac cgccgagcat cagtcgtttt acgacaccac caggaaattc
     2401 gtcgaggaag aaatcaaccc gcacgtcgcc gaatgggaag ccagcggcct gttcccggcc
     2461 aggcaggtgt tcaagaagat gggcgacctg ggcctgctgg gcatcagcaa gccggagagc
     2521 gtcggcggcc tgggcctgga ctactcgtac cagatcatgt tttccgaagc gctgggcaac
     2581 tgcacctgcg gcggcattcc catggcgatc ggcgtgcaga ccgacatggc gacgccggcg
     2641 ctggcgcgct tcggctcgga ctacctgaag gaaaacttcc tcaagcccgc ggtggccggc
     2701 gacatggtcg cctgcatcgc ggtgagcgaa ccgggcgcgg gctccgatgt ggccggcgtc
     2761 aagaccaccg cacgcaaggt cggcggcgac tacgtcatca gcggcagcaa gatgtggatc
     2821 accaacggca cccagggcga ttttgcctgc ctgctggcga acaccgggga accggtcgac
     2881 ggcggccgtc acggcaacaa gagcctgatc gtggtgccgc tcgacgccaa gggcatcgac
     2941 cgcaagacca agctcgacaa gctgggccag cgctcgtccg acaccgcgat gatcttcctc
     3001 gacgaagtga aggtgccggc gaagaacctc atcggcgaag agggcaaggg ctttgtctac
     3061 cagatgatgc aattccagga agagcgcctg ttcctgtcgg cctcgatcct gcgcgccatg
     3121 gaaatctgcg tcgagcagac ggcgacttat acccggcaac ggcaagcctt cggccagagc
     3181 gtgctggaca accagtacgt gcacttccgc ctgtccgagc ttgctacgga aatcgaagca
     3241 tgccgctcgc tgctgtaccg ggcgaccgag gaactggtgc gcggcggcga cgtgacgctg
     3301 ctggcctcga tgaccaagct gaagaccgcg cgcgtggcgc gcgaggtggc cgacggttgc
     3361 ctgcagtact ggggcggcca gggcttcatg tgggaaaacc aggtgagcca gttctaccgc
     3421 gatctcagat tgcactcgat cggcggtggc gccgacgaag tgatgctgtc catcatcagc
     3481 aagcgcctgg gcctgggcgg aaagaagaaa aaatgaacct gccggaaagc aagagcctgc
     3541 tgctgcgcgc cgagggcggc gtgctgcacc tgacgctgaa ccgcccggag tcgcgcaacg
     3601 cgatgtcgcc ggagatgctg gcggagatcg aagcggcgtt cgattcgatt gcaggatcga
     3661 gcgaaatccg catcgtcgtg ctgcgcggtg ccggcgggca tttctgcgcc ggagcggacc
     3721 tgaaaggcat gatgggtgcc ggcatgaagc cgccggctcc tggacagccc gatccgctgg
     3781 ccgcgatcaa ccgtgccttc ggcaccatgc tgcgcaaggt cgaggcggcg ccgcaggtgg
     3841 tgatcgcggt atgcgaaggc ggtgtgctcg gcggtggctt cgggctggcc tgcgtgtccg
     3901 acatcgcgct ggcgcatacc gaggcgaaat tcggcatgcc ggaaacctcg cgcggcctgc
     3961 cgcccgcgca gatcgcgccg ttcgtggtcg agcgcatcgg cctgacgcag gcgcgccgcc
     4021 tggcgctgac cagcgcgcag ttccgcggcg aggaggcatt gaagctcggg ctggtgcacg
     4081 aggtgttcgg caacgaggaa gagctgaaca ccaagctcgg cgcttacatc aagcaggtgc
     4141 tgaactgcgc gccacaggcc aatgcactga ccaagcagat catgctgcag gccgggaagg
     4201 ttccgatgga ccaggtgctc gacgacgcag cggccaagtt cgccgcctgc gcgcgcggat
     4261 ccgaagcgcc ggaaggcatt gcggctttca tgcagaaacg cgcgccgaag tgggcgcaat
     4321 aagagaacgg caacgatgaa gaaattcgac aaggtgctgg tggccaaccg cggcgagatc
     4381 gccgtgcgcg tgatccgcgg cgcgaagaag ctcggctaca ggaccgtggc ggtgtacagc
     4441 gacgccgaca aggacagcct gcacgtgcgc gaggccgacg aggcggtgcg catcggcccg
     4501 ccggcggcca aggagtcgta cctggtcatc gacaacatat tggccgccgc gaagcagtcg
     4561 ggcgcgcagg cgatccaccc gggctacggc tttctgtccg agcgcgcgga gttctccacg
     4621 gcggtgcacg atgccggcct gaccttcatc ggccccgacg cgttttccat cgacgccatg
     4681 ggcaacaaga gcgcgtcgaa gatccgcatg atcgcggccg gcgtgccgtg cgtgccgggc
     4741 taccagggcg acgaccaggc cgacgaaacg ctgcgcaagg aagcagtgaa ggccggcctg
     4801 ccggtgatga tcaaggcagc ggccggcggc ggcgggcgcg gcatgcgcct ggtgcacgac
     4861 gagaaggaat tgctcgccaa catccagtcg gcgcgcagcg aggccaccaa tgccttcggc
     4921 agcggccagc tgctgatcga gaaggcggtg atggacgcgc gccacgtcga gatccaggtc
     4981 ttcggcgacc gccacggcaa cgtggtgcat ttcggcgaac gcgactgctc ggtgcagcgt
     5041 cgcaaccaga aggtcgtcga agaagcgcca tcccccgcgg tcgacgatgc gctgcgcctg
     5101 aagatgggcg cggccgcggt ggccgcggcc aaggcggtga actacgtcgg cgccggcacg
     5161 gtggaattca tgctggcgcg cgacggccag ttctacttcc tggagatgaa cacgcgcctg
     5221 caggtggagc acccggtgac cgaactggtc tacggcgtgg acctggtgga gtggcagctg
     5281 cgcgtggcgc agggcgagaa actgccgctg acgcaggaag aaatcctcaa gcgcaggaac
     5341 ggctgggcca tcgaggtgcg gctgtgcgcc gaagatccgc gcgacaacta catgccgcag
     5401 gccggcccgg tgctggcctg gcgggcgccg cagggcgccg gcgtgcgcgt ggacacctac
     5461 ttggaaagcg gctgccgcat cagcccgtac tacgactcga tgcaggccaa gatcatcgcc
     5521 tatggcgaca cgcgtgagtc tgcgcgccag cgtctgctca aggcgctgga cgaaaccgtg
     5581 ctgctgggcg tgatcaacaa caaggattat ctgcacgcga tcctatcgca cgaagcattt
     5641 gccggcggcg atttctcgac cgccttcatc ggcaagtatt tcccggccga cgcggtggcc
     5701 aggtctgcgc ccacgcaccg gcacatcgcg ctggccgcgg tggcgctgta catggacgac
     5761 gcgcaccgtc tcgccgccga gcatgggctg acgcaggaat tcgtcggctg gcacagttcg
     5821 catgagaccc cggccgattt caaggtgaag tggcgcgacc gcgcctataa cctcgaagtg
     5881 ctggtgcacg acggccacct gttcaccacc acagttgaag gcgagaaaat cgagatcgat
     5941 gtcacgcgca gcagcgagca cgatttccag tacctctgcg caggcgtgca ggcgaccgcg
     6001 cgcatcgcgc acgacggcga catggtgtgg atcagcgccg aaggcgcgac gcacggctac
     6061 accgacgtca ccctggcgcc ggtgcagggc gcggctgcag gttcggacgg ccgcctgctg
     6121 gccaactccg acggcaaggt ggtcgcggtg cacgtgaaac ctggtgacgt gatcgccaag
     6181 ggccagacgc tggccgtgct ggaagcgatg aagatggaat tccagctggc gctgccggtg
     6241 ggcggcacgg tggaagcggt cagcgtgagc gcaggccagc aggtgaagaa ccgccagctg
     6301 ctggttcagg tgaagccggc gtagccggct tcatcccgtc gcaaaaatct ggatggtttt
     6361 ttccttctcc catcgggaga aggtggcgcg cagcgccgga tgacggattc acgaggctat
     6421 gtcattcatt gctagcgtgc ccctcacccg cgcctgcagc gcagcctctc ccggagggag
     6481 aggcattgcg gcgtcccgaa atcctcaacg ctgagcctga acggctgggc tggatgccgc
     6541 gaacttttca atccaaaagc ggataccgca ccgccgttcg gtatgttatt aaatatttat
     6601 tgatttaata ataaaacaat aaataataac aacgcaggac cgggtgcggt gaggcatttt
     6661 cgcgggtgca gaagcgctgc gcggcccgcc gacgcgcgca ttcatatcgc agcatcatcg
     6721 ccaataaaaa acccgccggc tcgcgccggc gggttgcaat actccctgat cgaccgcggc
     6781 ttactgcgtc tgctcgatca ccttgagctc gacgcggcgg ttttcttcgc ggccttcttc
     6841 ggtgtcgttg gtcgcgatgg gctgggtctc gccgtagccc accgggttca tgcgggccgc
     6901 agccacgccc ttgccggtga ggtagttctt gaccgagttg gagcggcgct ccgacaggcc
     6961 gaggttgtag gcatcggtgc cgatgttgtc ggtgtggccg cccagctcga cgttgatctc
     7021 aggatagttg ttcagcgtgg tggccacgcc atcgaggatc ttcttcgcat cctgcgtcag
     7081 gcgatcggag tcgaactcga acttgacgcc gcgcagaatg aaggtcttct ccagcgcgca
     7141 gccgttggcg tccacttcct cacccgggcg cggcttgcgg cagtcgccga ccggcgcgca
     7201 gccgttgggc aggatcttgg cgccggccgg ggtgtgcggg cactcatcca ggtaatccgg
     7261 gatgccgtcg ccgtcgctgt ccagcgggca gccgttggcg tccacctgca cgcccttggg
     7321 cgtgttcggg cacttgtcga ggtaatccgg cacgccatca ccgtcgctgt cgagcgccgg
     7381 gcagccgttg gcgtcgacca cggtaccctt cggagtgttc gggcacttgt cgaggtagtc
     7441 gggcacgccg tcaccgtcgc tgtccagcgg gcagccgttg gtgtccaccg ccacgccctt
     7501 gggcgtattc gggcactggt cgcggctgtc gggcacgccg tcaccgtcgc tgtccagttc
     7561 gcagccgttg gcgtccacct tcacgccggc cggcgtgttg gggcacttgt ccatgtaatc
     7621 gggcacgccg tcgccgtcgg aatccagcgg gcagccttcg gcatccatcg gcacgccctt
     7681 gggcgcgttc gggcacttgg ggttcttgcc gctccagctg ccgaacgcgt agtgcatgcc
     7741 gagcatgatc gagttgttct tgtagctggt gtccagctcg gtgccgccgc cgaaatcgaa
     7801 agcgacgttg ttggcggcca ggtagcggta gtcgatcgac aggcgcgtat ggtcggtgac
     7861 cttgaagttc acgcccgcgc ccagctgcca ggagaacacc gtcgagctct tgttgatcgg
     7921 gccgaaggcg gtgccggcgt tgctgccgga aaagtccttg tactgcacct tggtggcgcc
     7981 caggccggcg ccgatatacg ggtctacgcc agcagtgggc gcaaagtcgt accacaggtt
     8041 gccgatggcg ccgtaagact gggccttgcc ggcgacattg gtcgcggtga tcgaggtggt
     8101 ggtcggtgcc gaggtgacgc cgccggtggt ggtggtcgtg gtgacgttgg tcaccgcgga
     8161 ttcgatgtcg ttgttgcggt agctgagttc aaactccggg cgcaggccgt tggccgcctg
     8221 gtagccgaag cccaggccga aggtgctgcc gcccttgaac ttcaccgtct gcgaggtgcc
     8281 ggtgacagtg gtggtgctgc cgccggtggc gcagagcagc gcgcccagcg gggtggggca
     8341 cagggcgccg gccagcgggt tgagcagcgg gtccagcggc gtccccgccg ggatggcgat
     8401 caggccgagg atgttgagcg aggtcgtgct gggcgtcgac gtgctgttgc tgaccgtgtt
     8461 gtcggccttc gacagcgagt tgaatccgcc attcaggttg atgtaccaac ccggatcgaa
     8521 cgtcgcctgg tcggcctgcg ccggtgcggt agcgagcgcg gctaaggcag ccgcagcgga
     8581 aaagacgatg gttttgcccc acatgttctt gcgcataagc tccaaccttt gcttttttac
     8641 tgttaccgct ggcgtgccac gatgcgacgc agagtgggcg gcattctaga ggatttctca
     8701 cagcaacgac cactggaaaa cccccagcgg agcgctgcat ccaagaagcc gggctcgact
     8761 gccgcgcgat tggaccgccg gcgcggccaa tcggcagcac tatttgttgt tgtcgggcag
     8821 gttgccgcag taagtcagta acccagctgg cgggcggcga ggtccttcat gatctcgatt
     8881 gacccgccgc cgatcgacag caccttggtc tcgcggtaga tgcgctccac tttggccccg
     8941 cgcaggtagc cggcgccgcc gaagatctgc atggcctcgt tggccaccca ttccagcgag
     9001 gtggtggcga ggttcttcag catgcacacc tccgccaccg gcatgtgctt ctggccgatg
     9061 cgccaggcca ggtgatcgag gttggccttc accgcctcgt tgcgcatctt catgtccacc
     9121 agctggtggc
//
LOCUS       DASZZC010000734        10962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_693896,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000734 DASZZC010000000
VERSION     DASZZC010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693896"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VHE37_15650"
     CDS             complement(<1..437)
                     /locus_tag="VHE37_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HVT37024.1"
                     /translation="MVRVSGGAARAIAKTICGFEPSPRRARLTDFHDADGATIDRGHA
                     LFFPAPHSFTGEDVLELHAHGSPVVLEALVRRACELGARPARAGEFSERAFLNGKLDL
                     AQAEAVADLIAAHSEAQARAALRSLDGAFSRRVEALVHTIVQLR"
     gene            complement(513..2234)
                     /gene="yidC"
                     /locus_tag="VHE37_15655"
     CDS             complement(513..2234)
                     /gene="yidC"
                     /locus_tag="VHE37_15655"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HVT37025.1"
                     /translation="MINQTRTLLVVALFFVAYLLWQAWQHDYPSVPPPAPPASAQVAA
                     TASAVPESASIPTAAGSVAAPPAESTAPAGNLSANQTPKQDLVTANNDVLKLTIDPRG
                     GTIVGAALLDYPQQVKRGSPPVQLLDDATANYYVAQDGLIGANGSAAPNHLAEFKPQA
                     TSYSLESGQKQVVVTLTWDDAATGVSVVKRYTLTRGSYVVELSQQVENHGSKTWQGNT
                     YEQLDRIEPPPPPKHLLEFYNPDSYAFTGAAWYSDKYNKLKFGDFEKDPLHARVTGGW
                     IAMSQRFFVAAWIPDAKLAQQFGSVETPATDANGKPRYLIRSVGPGFSVAPGASATRT
                     SRLYVGPKLQDRLGDVAPGLDRTVDYGMWTFIAQPMFKLLAFLHKLVGAWGLAIIVLV
                     LLIQAATWKLTEAQYKAGARMRKLQPRLKALKERFGDDKQKYQQAMMELYKKEKINPM
                     AGCLPALITFPIFFALYYVLIFSVELRHAAFLWIPDLSAADPLFILPVVYALTMLGQQ
                     FLTPVQGMDPTQARIMKAMPVLFAVLFFFFPAGLTLYYVVRGICMLGQQWYITRKIDR
                     ADARARA"
     gene            complement(2274..2645)
                     /gene="rnpA"
                     /locus_tag="VHE37_15660"
     CDS             complement(2274..2645)
                     /gene="rnpA"
                     /locus_tag="VHE37_15660"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HVT37026.1"
                     /translation="MAGLPPAARLRRASDFAALRNAERWQGRHFLLRWLKLPEGNARL
                     GLAVSRKVSKRAVERNRIKRVVRESFRAEREGLPALDILVIPRPSAAGTPNPVLFADL
                     GLAWCKLKTLKQTATPGTIGG"
     gene            complement(2656..2790)
                     /gene="rpmH"
                     /locus_tag="VHE37_15665"
     CDS             complement(2656..2790)
                     /gene="rpmH"
                     /locus_tag="VHE37_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HVT37027.1"
                     /translation="MKRTYQPSVLKRARTHGFRARMKTADGRKVLSARRAKGRKRLIP
                     "
     gene            3004..4314
                     /gene="dnaA"
                     /locus_tag="VHE37_15670"
     CDS             3004..4314
                     /gene="dnaA"
                     /locus_tag="VHE37_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HVT37028.1"
                     /translation="MSDLWQRCLARLEGEIEPGVLHTWLQPLQARYDDGMLRLLAPNS
                     YTLEFVRNEYLARIEGTLSALAGHPVPLKLEVGSLTPVARPQVVPGSAATPAPQHNID
                     PHYTFDSFVEGKSNQLGKAAAMQVAQNPGRAYNPLLLYGGTGLGKTHLMQAAGNLMLK
                     HNPSMKLLYVHSERFTEMMITALRNKNMDDFKHRFRSVDALLIDDIQFFAGKDATQEE
                     FFHTFNTLYESRQQIILTCDRYPKEVDNLELRLKSRLGWGLSVAIEPPDFETRAAILL
                     EKAQTRGIPLPGDVALLLARRIRSNVRELEGALNTVAAQANLSGRGITMELVQETLRD
                     LFNVHARAITIQNIQKTVADYYQLRLSDLLSQRRVRSLARPRQIAMALAKELTEHSLP
                     EIGEAFGGKDHTTVMHACKKIRSLCDTDMRLRQDWDKLIRILTG"
     gene            4521..5621
                     /gene="dnaN"
                     /locus_tag="VHE37_15675"
     CDS             4521..5621
                     /gene="dnaN"
                     /locus_tag="VHE37_15675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVT37029.1"
                     /translation="MRFSTQRETLLKPMQQVVGVVERRQTLPVLAHLLVQVKEGRLSL
                     TGTDLEVEMRASTSVEMAAAGETTIPARKWFDLVRALPDGVNVSVELKGDRVVMHAGR
                     SRYTLATLPAEEFPNNDEIEITDKVSLPEGTLRRLMERTSFAMANQDVRYYLNGLLLD
                     LRGAELRCVATDGHRMAMAQTNLDKPAGSARQLIIPRKGVTELQGLFENADNPVEIEF
                     SSNHLRVRRSDVVFTSKLIDGRFPDYEAVVPLGADKIVKIDRDELRNALQRAAILSNE
                     KYRGVRLEVTPGCLRIIAHNPEQEEAFEEIEADTKVGDVAIGFNVGYLLDALGALDEA
                     KVQLQLRDAQSSCLLRGVSSQEAQHVVMPLRL"
     gene            5657..6739
                     /gene="recF"
                     /locus_tag="VHE37_15680"
     CDS             5657..6739
                     /gene="recF"
                     /locus_tag="VHE37_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HVT37030.1"
                     /translation="MNIELLRADNLRCFEHVSFEPGPGINWLIGPNGVGKTTVLEAAY
                     ILSHGRSFRPGGRTAPRRQGASEYLVYAEARRSGQPKVRLGLTRKDDSWTGRLNGEDL
                     PNLAPLFEACPVVYFGPESQTLILGPAEERRAFLDWSVFHVEHASLELWKAWRRTLRQ
                     RNALLRQGAPSGAFAPWEYDLGRLAREIHALRERCLVGLQRYIAREAGALVPELGEVH
                     IEYHAGWDVQKGLEQQLVAARERDREAGFTRHGAHRADWTLAFHDVAKREHLSRGQAK
                     AAALVCTLALARWLKESIGDYPLLCVDDLESELDAPHAARVIQWLQEAVIQCWLTATQ
                     VPDSSHLAASAKLFHVKRSGCERFHA"
     gene            6809..9220
                     /gene="gyrB"
                     /locus_tag="VHE37_15685"
     CDS             6809..9220
                     /gene="gyrB"
                     /locus_tag="VHE37_15685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HVT37031.1"
                     /translation="MNENYDSSSIKVLKGLEAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGYCDHIEVTIHDDGTVSVTDNGRGIPVDIHPEEGRSTAEVVMTVLHAGG
                     KFDANSYKVSGGLHGVGVSVVNALSEHLWLTIYRENQEYRQEYRLGEPLYPVREVGPS
                     NRRGTTVRFLPSSGTFTNIEFQYDILAKRLRELAFLNSGVKITLEDQRVAKKDVFEYE
                     GGIRAFVKHLSQLKTPLHPNAIGFSVQSGDTTVELAMQWTDGYQESIFCFTNNIPQKD
                     GGTHLTGLRSALTRTLSNYIEQTGLAKNSKVALSGDDMREGLIAVLSVKVPDPKFSSQ
                     TKDKLVSSEVKSIVEQAVNTKLEEYLQENPGDAKLIAGKVVDAARAREAARKARELTR
                     RKGALDIAGLPGKLADCQERDPALSELFLVEGDSAGGSAKQGRNRKTQAVLPLKGKIL
                     NVEKARFDKMLNSAEVGTLITALGTGIGKDEFNVEKLRYHRIIIMTDADVDGSHIRTL
                     LLTFFYRQMPELIERGHVYIGLPPLYKIKQGKNELYLKDDAALNEYLIASAVQDAAWI
                     PGAGATAVEGAALETLLRQYQAGLDQIGRLGQRGDASAFTAMLEYTPLEDDMWNDLAA
                     MRAWADGLTSKLNATGLGRPQFRLDLREAAGEHPAAWLLRRAQHGSEHTWVLPRSFFS
                     GADFRPLLEVARALHGRGTTDAEIRRGNAQQTVGSFADARAWLFEEAKKGRQIQRFKG
                     LGEMNPEQLWETTVNPDTRRLLQVRIEDALEADQIFSTLMGDVVEPRREFIETNALRV
                     GNLDV"
     gene            9453..10700
                     /locus_tag="VHE37_15690"
     CDS             9453..10700
                     /locus_tag="VHE37_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT37032.1"
                     /translation="MLQHKCGQQQQQQKSDKQDKQKTKYPNATRKQPKNIGVDAKEAK
                     GLNEGLAAANSGDSATALKDLQPIADSSSSAYAKALAMLGLAQVKYRGGDTKGAITLQ
                     KQALDSNALDNDSYFQGLETLAQMYIADEDYSDALTTLDTWNKESGAQSADVYALQGN
                     AYYRLQKYPQAVAAIQKAKSLSDKPQASWDQIEMASDFAMDKYDDAAKLAEAALAKDP
                     NNSTLLQNVIAIYINAHQDQKALALLERARANGQIKDQAGYMNMAKMYDNLGQNSANP
                     KANAMKAVGVLQEGMNKGIIQPGYDSYKLLGDSYAIAGDYKNAVAAYGKAAPDAKTGE
                     VDFLRGQMLEQLGRHKEARDALKQAVAKGNFKRIGAAYLTLGNAELALKDKAAARAAY
                     EQAEQDPETRADAQRTLKQLGHK"
BASE COUNT         2241 a         3326 c         3436 g         1959 t
ORIGIN      
        1 cgcagctgaa cgatggtatg caccaatgcc tcgacgcgac gcgaaaatgc gccatccagc
       61 gaacgcaacg cagcgcgcgc ctgtgcttcc gaatgcgcgg cgatcaggtc ggcaaccgct
      121 tcggcctgcg ccaggtcgag cttgccgttc aggaacgcgc gttcggaaaa ctcgcccgcg
      181 cgtgcgggcc gcgcgccgag ctcgcaggcg cgtcgcacca gcgcctccag caccaccggg
      241 ctgccatgcg catgcagttc cagcacgtct tcgccggtga aggaatgcgg cgcggggaaa
      301 aacagcgcgt ggccgcgatc gatcgtcgcg ccatcggcat catggaagtc cgtcagccgc
      361 gcccggcgcg gcgacggctc gaaaccgcag atcgtcttcg cgatcgcacg cgccgcgcca
      421 cccgaaaccc tcaccacgcc gatgcccccg cgcccgttcg gggtcgcgac ggcaacgatg
      481 gtttcagcag gctcgcgcct ggcggcgccg catcaggccc gcgcgcgcgc gtcggcccgg
      541 tcgatcttgc gggtgatgta ccactgctgg ccgagcatgc agatgccgcg cacgacgtag
      601 tagagcgtga ggcccgccgg gaagaagaag aacaacaccg cgaacagcac cggcatcgcc
      661 ttcatgatcc tggcctgggt cgggtccatg ccctgcaccg gcgtcaggaa ctgctggccc
      721 agcatcgtca gcgcgtacac gaccggcagg atgaacagcg gatcggcggc cgagaggtcc
      781 gggatccaca ggaacgccgc gtggcgcagt tcgacgctga agatcagcac gtagtacaac
      841 gcaaagaaga tcgggaaggt gatcagcgcc ggcaggcagc ccgccatcgg attgatcttt
      901 tccttcttgt acagctccat catcgcctgc tggtacttct gcttgtcgtc gccgaaacgc
      961 tccttcagcg ccttcaggcg cggctgcagt ttgcgcatgc gcgcaccggc cttgtactgc
     1021 gcctcggtaa gcttccaggt cgcggcctgg atcagcagca ccagcacgat gatcgcgaga
     1081 ccccacgcgc ccaccagctt gtgcaggaac gcgagcagct tgaacatcgg ctgcgcgatg
     1141 aaggtccaca tgccgtaatc gaccgtgcgg tcgaggcccg gggccacgtc gccgagccga
     1201 tcttgcagtt tcgggccgac gtacaggcgc gacgtgcgcg tcgcgctcgc gcccggtgcg
     1261 accgagaaac ccgggccgac gctgcggatc aaataacgcg gtttgccgtt cgcatcggtg
     1321 gccggcgttt ccacgctgcc gaattgctgc gccagcttgg catccggaat ccacgccgcc
     1381 acgaagaagc gctggctcat cgcgatccag ccaccggtga cgcgcgcgtg cagcggatcc
     1441 ttttcgaagt cgccgaattt cagcttgttg tacttgtcgc tgtaccaggc ggcgccggtg
     1501 aacgcgtagc tgtcggggtt gtagaactcg agcaggtgct tgggcggcgg cggcggctcg
     1561 atccggtcga gctgttcata cgtgttgcct tgccacgttt tgctgccgtg gttttcgacc
     1621 tgctgcgaga gttcgacgac ataactgccg cgggtcagcg tgtaacgctt gacgacgctg
     1681 acgccggtgg cggcatcgtc ccatgtcaac gtgaccacga cctgcttctg ccccgactcc
     1741 aatgaatacg aagtcgcttg gggcttgaac tcagcgaggt gattgggcgc ggcgctgcca
     1801 ttcgcgccga tcaggccgtc ctgcgccacg taatagttcg cggttgcatc atccagcaac
     1861 tgcaccggcg ggctgccgcg cttcacctgc tgcggataat cgagcaacgc ggcgccaacg
     1921 atcgtgccgc cgcgcggatc gatcgtgagt ttcaacacgt cgttgttggc ggtgacgaga
     1981 tcctgcttcg gcgtctgatt cgcgctgagg ttgccggcgg gtgctgttga ctccgcgggc
     2041 ggtgccgcga cgctgcccgc tgccgtgggg atcgaagcgc tttccgggac cgcagacgcc
     2101 gtcgcggcaa cctgcgcaga agcgggtggt gccggaggcg gcacggaggg gtagtcgtgt
     2161 tgccaggcct gccacagcag gtaggcgacg aagaacaacg ccacgaccag cagggtgcgt
     2221 gtttgattga tcactgggga actcgggctg gggcgctcag ggcccggagc aagtcagccg
     2281 ccgattgtgc cgggtgtggc cgtttgcttc aatgttttga gcttgcacca ggcaagcccg
     2341 agatcggcaa acagcacggg gttcggagta ccggctgccg acggacgcgg gatgaccagg
     2401 atatccagcg ccggcaatcc ctcgcgctct gcccggaatg attcgcgaac gacacgcttg
     2461 atccggttgc gctccaccgc gcgcttgctg accttgcgcg agactgcaag acccagccgg
     2521 gcgtttcctt ccgggagctt gagccaacgc aacaggaagt gccttccctg ccagcgctcg
     2581 gcgttccgca acgcggcgaa atcggaagca cggcgcagcc gcgcagcggg cggcaagccg
     2641 gccatgccgg cgtgcttacg ggatcaggcg cttgcgaccc ttggcgcgac gcgcggacag
     2701 taccttgcgg ccgtcggcag ttttcatgcg tgcacggaag ccgtgggtgc gggcgcgctt
     2761 gagcacgctg ggctggtagg tgcgtttcat gggtgactcc gcagcgggat gcccgcgcaa
     2821 ggcgggtcag acatggaaaa agaccgtgga ttttagggtt gaatgcggtc gccagtcaat
     2881 cccgagcggg cacgatcgtc gaggacgacg acgcgttcgc aggtttgcgg cagcctgata
     2941 gactcccctg ttctgccgcg cgtgtttgca cgtcggcgtt cctgatggtt ttgcgcggac
     3001 tccatgagcg atctttggca acgctgcctc gcccggctcg aaggtgaaat cgagccgggc
     3061 gtgctgcata cctggctgca gccgctgcag gcgcgctatg acgatggcat gttgcggctg
     3121 ctggcgccga acagctacac gctggaattc gtgcgcaacg aatatctggc gcgcatcgag
     3181 ggcaccctgt ccgcgcttgc gggtcatccc gtgccattga agctcgaagt gggctccctg
     3241 acccccgtcg cgcggccgca ggtggtaccg gggtccgcgg ccacgcccgc gccgcaacac
     3301 aacatcgatc cgcactacac cttcgatagt ttcgtcgagg gcaagtccaa ccagttgggc
     3361 aaggccgcgg cgatgcaggt ggcccagaat ccggggcgcg cctacaaccc gttgctgctt
     3421 tatggcggca ccggcctcgg caagacgcac ctgatgcagg cggcgggcaa cctgatgctg
     3481 aagcacaacc cgtcgatgaa gttgttgtac gtgcattccg agcgcttcac cgaaatgatg
     3541 atcacggcgt tgcgcaacaa gaacatggat gacttcaagc atcgcttccg ttcggtggac
     3601 gcattgctga tcgacgacat ccagttcttc gctggcaagg atgcgaccca ggaagagttc
     3661 ttccacacct tcaacacgct gtacgaatcg cgccagcaga tcatcctgac ctgcgaccgg
     3721 tatccgaagg aggtggacaa tctcgagctg cgcctgaaat cccgtctcgg ttggggtttg
     3781 tcggtggcga tcgaaccgcc tgatttcgaa acccgtgccg cgatcctgct ggaaaaggcg
     3841 cagacgcgcg gcatcccgtt gcccggtgac gtggcgctgc tgctggcgcg ccggatccgt
     3901 tccaacgtgc gcgaactgga aggcgccctc aacaccgttg ccgcgcaagc gaacttgtcg
     3961 ggacgtggca tcacgatgga actggtgcag gaaaccctgc gcgacctgtt caacgtccac
     4021 gcgcgcgcca tcactatcca gaacatccag aagacagtcg ccgattatta ccagttgcgc
     4081 ctttccgacc tgctctcgca gcgccgggtg cgctcgctgg cccggccgcg acagatcgcg
     4141 atggcacttg ccaaggaatt gaccgagcac agcctgcccg aaatcggcga ggccttcggc
     4201 ggcaaggacc acaccacggt catgcatgcc tgcaagaaga ttcgcagcct ctgcgacacc
     4261 gatatgcgct tgcgccagga ctgggacaaa ttgattcgga tattgaccgg gtaaacttgt
     4321 ggacaagaat gggatggttg ttcgtcgcca aatcatccac aattcgtaca ccggaagcac
     4381 aagccgaagg ttcgaggctt ttggtgatca agctattgat ttataagagc taaatttgaa
     4441 acagcagtta tcaacgtcgc ctgcttctac cagcttgttt acaagtaaat ccagttagag
     4501 attcgcctcg gggactaacc atgcgcttca gcactcagcg ggaaaccttg ctcaaaccca
     4561 tgcagcaggt cgtcggtgtg gtcgagcgca gacagacctt gccggtactt gctcacctgc
     4621 ttgtccaggt caaggaaggg cgcctgtcgc tgacgggaac cgacctcgaa gtcgaaatgc
     4681 gcgccagcac ttcggtggag atggcagccg ccggagaaac cacgattccc gcgaggaaat
     4741 ggttcgatct ggttcgtgcc ctccctgacg gcgtgaatgt cagcgtcgag ctcaagggcg
     4801 accgtgtcgt catgcatgcc ggtcgtagcc gttacacctt ggcgacccta cccgctgagg
     4861 aatttccgaa caacgacgag atcgaaatta ccgacaaggt ctcgttgccg gaaggtacct
     4921 tgcgtcgctt gatggagcgc acctccttcg cgatggccaa ccaggatgtc cggtactatc
     4981 tcaacggatt gctgctggac ttgcgcggcg ccgagttgcg ttgcgtggcc acggacgggc
     5041 accgcatggc gatggctcaa acgaatctcg acaagcccgc ggggagtgcc cggcagttga
     5101 tcattccccg caagggcgtc acggagttgc agggtctgtt cgagaacgcc gacaaccctg
     5161 tcgaaatcga gttttcgagc aatcacctgc gggtgcgccg ctccgacgtc gtgttcacct
     5221 ccaaattgat cgacggccgg tttccggact acgaagcagt cgtgccgctg ggagccgaca
     5281 agatcgtcaa gatcgatcgc gacgaactcc gcaatgcatt gcagcgcgcg gcgattctgt
     5341 ccaacgagaa atatcgcggg gtgcgcctgg aagtcacgcc gggctgcctg cgcatcattg
     5401 cccacaaccc cgaacaggaa gaagctttcg aagagatcga agcggacacc aaggtgggcg
     5461 atgtcgccat tggtttcaac gtaggctatc tgctggatgc actcggagcc ttggacgaag
     5521 ccaaggtgca gctgcaattg cgtgacgccc aatccagttg cctgcttcgt ggcgtctcca
     5581 gtcaggaagc ccagcacgtc gtgatgccgt tgcgtttgta aggcagccgg agggacgtag
     5641 acgaagcttc gagtccgtga atatcgaact gttgcgtgcg gacaatctgc gttgcttcga
     5701 gcacgtgagc tttgagcccg gtcccggaat caattggttg attggaccga atggtgtcgg
     5761 caagaccacg gtgctggagg ccgcctacat cctttcccac ggccgttcct tcaggcctgg
     5821 cggtcgaacc gctcccaggc gtcaaggggc aagcgaatat ctcgtctatg ccgaagcccg
     5881 tcgatcaggc caaccaaagg tgaggcttgg cctgacgcga aaggatgaca gctggaccgg
     5941 gcggctcaat ggcgaagatc tgcccaatct cgcgccgctg ttcgaagctt gccccgtggt
     6001 ttacttcgga cccgagagcc agaccttgat tctcggtccg gccgaggaac gaagggcatt
     6061 tctggattgg agcgtgttcc acgtggaaca cgcatccctg gaattgtgga aagcgtggcg
     6121 cagaactttg cgtcagcgca acgcgttgct tcggcaaggg gcaccctccg gcgcttttgc
     6181 tccctgggaa tatgacttgg gccggctggc gcgggaaatt cacgcattgc gagagcgttg
     6241 tctcgtggga ttgcaacgct acatcgcccg tgaggccggc gccttggttc ccgaattggg
     6301 agaggtccat atcgaatatc acgcaggctg ggatgtacaa aaaggattgg aacagcagct
     6361 ggtagcagcg cgggagcggg accgtgaagc aggattcacc cggcacgggg cccaccgcgc
     6421 ggactggaca ttggcattcc atgacgtcgc caagcgtgag cacctgtcac gcggccaagc
     6481 caaggcggca gccttggtgt gcaccctggc gcttgcacga tggctcaagg agagcatcgg
     6541 tgactatccg ctgctatgcg ttgatgatct ggaatccgag ttggacgcac ctcacgccgc
     6601 acgagtcatt caatggcttc aagaagcggt catccagtgt tggctgacgg cgacccaggt
     6661 accggattcc agtcacctgg ctgcaagcgc aaaactgttc cacgtgaaac gttctggatg
     6721 tgagcgcttc cacgcgtaac tcgaaggcaa ttggggcgag aggtataatg aaccgtttgg
     6781 gtcgcctcca gcacctcaga gatccgtcat gaatgagaat tacgattcca gcagcatcaa
     6841 agtcctgaaa gggctcgagg cagttcgcaa gcgtcccggc atgtatatcg gcgacaccga
     6901 tgatggtacg ggcctgcacc acatggtttt cgaggtcgtg gacaatgcga tcgatgaggc
     6961 gttggccggc tattgcgatc acatcgaggt cacgatccac gatgacggta cagtgagcgt
     7021 gacagataat ggccggggta ttccggtgga tattcacccc gaggagggcc gttcgactgc
     7081 cgaggtcgtc atgacggttc tgcacgcggg cggcaagttc gacgccaatt cctacaaggt
     7141 ttcaggcggc ctccatggcg tgggcgtttc cgttgtcaac gcgctgtccg agcacctttg
     7201 gctcacgatt taccgcgaga accaggaata tcggcaggaa taccggttgg gggagcccct
     7261 ctatccggtg cgagaggtgg gcccttccaa tcgccgcggc acgacggttc ggtttctgcc
     7321 gagttccggg acattcacca acatcgaatt tcaatacgac attctcgcga agcgccttcg
     7381 ggagctggcg ttcctcaact ctggcgtcaa aataaccctt gaagatcaaa gggttgcgaa
     7441 gaaagacgtg ttcgagtacg agggcggaat tcgtgccttc gtcaagcacc tctcccagct
     7501 caaaaccccg ctgcacccca atgcaatcgg cttcagtgtc cagtcgggcg acaccaccgt
     7561 tgaattggcc atgcaatgga ccgatggtta ccaggaatcc atattctgtt tcaccaacaa
     7621 cattccgcag aaggatgggg gcacccatct gacgggattg cgcagcgcct tgacgcgaac
     7681 gctcagcaac tacatcgaac agaccggatt ggcgaagaat tccaaagtcg cgctgtctgg
     7741 cgacgacatg cgggagggct tgatcgcggt gctgtcggtc aaggtacccg atcccaagtt
     7801 ttcctcgcaa accaaggaca agctggtttc ttcagaggtg aagagcatcg tcgagcaggc
     7861 cgtcaacacc aagctcgagg aatacctaca ggagaatccc ggcgacgcca aactcatcgc
     7921 cggcaaggtg gtcgacgcgg cgcgtgcccg tgaggccgcg cgcaaggctc gcgaactgac
     7981 ccgccgcaag ggtgcgctgg acatcgccgg gctgcccggc aaattggccg actgccagga
     8041 acgcgatccc gcattatccg aactgttcct ggtcgagggt gattcggccg gcggttcggc
     8101 caaacaaggg cgcaaccgca agacccaggc cgtgctgccg ctcaagggca agatcctcaa
     8161 tgtcgagaag gcgcgcttcg acaagatgct caattccgcc gaggtgggaa cgctgatcac
     8221 ggcactcggt acgggcatcg gcaaggacga attcaacgtc gaaaagctgc gctaccaccg
     8281 gatcatcatc atgaccgacg cggacgtcga cggctcgcac atccgtacgc tgctgctcac
     8341 cttcttttac cgtcagatgc cggagttgat cgagcgcggc cacgtttata tcgggctgcc
     8401 gccgctgtac aaaatcaagc agggcaagaa cgagttgtac ctgaaagacg atgcagcatt
     8461 gaacgagtat ctgattgcca gcgcagtgca ggacgctgcc tggatcccgg gtgccggcgc
     8521 gaccgccgtc gaaggcgcgg cattggaaac cctgttgcgc cagtaccagg ccggcctcga
     8581 ccagatcggc aggctgggtc agcgtggcga tgccagcgca ttcacggcga tgctggaata
     8641 cacgccgctc gaagatgaca tgtggaatga cctggccgca atgcgtgcgt gggcggacgg
     8701 cttgacgtcc aaactcaacg ccacgggttt ggggcgtcca cagttccggc tcgacttgcg
     8761 ggaagctgcg ggcgaacacc cggcggcctg gttgctgcgg cgcgcgcaac atggctcgga
     8821 gcacacgtgg gtgctgccgc gcagtttctt ctccggtgcc gatttccggc cgctgctgga
     8881 agtggctcgc gcgctgcatg gtcgcggcac gacggatgcc gagattcgcc gcggcaatgc
     8941 gcagcagacg gtgggcagct ttgccgatgc acgcgcctgg ttgttcgagg aagccaagaa
     9001 gggacgccag atccagcgct tcaaggggct tggtgaaatg aacccggaac agctgtggga
     9061 aaccaccgtc aaccccgaca cccggcgcct gttgcaagta cgcatcgaag acgcgctcga
     9121 ggccgaccag attttctcga ccctgatggg cgacgtggtg gaaccgcggc gcgagttcat
     9181 cgaaaccaac gccctgcgag tcggcaatct ggacgtctaa aaaccactgg acagccacgc
     9241 atgaacgcgg aactgtccgg tacttgagct ttgcgtatgg cccgcgctac tctcaccctc
     9301 cccgttcgcg cgcatacggc tcccgcagag gcgttctgat gaaggtcgat tcccgttggc
     9361 tggtttgtgg catgtgctct gcttttgctc tggcgttttt gccgtcgacg gcatttgccc
     9421 aaatgggtgg ccagcagtgt tcggacatcc agatgctgca acacaagtgt ggtcagcagc
     9481 agcaacaaca gaaatccgac aagcaggaca aacagaaaac caaatacccc aacgccacgc
     9541 gcaaacagcc caagaacatc ggggtcgatg ccaaggaggc gaagggcctc aacgagggat
     9601 tggcggcagc caacagcgga gactccgcga cggcgctcaa ggacttgcaa cccatcgccg
     9661 attcgagcag cagcgcctac gccaaggcgc tggccatgct gggcctcgcg caggtgaagt
     9721 accgcggggg cgacaccaaa ggtgcgatca cgctgcagaa gcaggcgctc gattccaacg
     9781 cactcgacaa cgacagctac ttccagggtc ttgagactct cgcccagatg tacatcgcgg
     9841 acgaagacta cagcgatgcg ctgacgacgc tggatacctg gaacaaggaa agcggcgcgc
     9901 aatctgcaga cgtgtacgcg ctgcaaggca acgcctacta tcgcctgcag aaatatccgc
     9961 aggctgtcgc ggccatccag aaggccaaat cgctgagcga caaaccgcag gcgagctggg
    10021 accagatcga gatggccagc gatttcgcga tggacaagta cgatgacgcg gccaagcttg
    10081 cggaagcggc attggccaaa gaccccaaca acagcaccct gctgcagaac gtcatcgcga
    10141 tctacatcaa cgcgcatcag gatcagaagg cattggcgct gctcgaacgc gcgcgcgcga
    10201 acggccagat caaggaccag gccggctaca tgaacatggc caagatgtat gacaacctgg
    10261 gccagaacag tgcgaatccc aaggccaacg cgatgaaggc cgtgggcgtc ctgcaggaag
    10321 gcatgaacaa gggcatcatc cagccgggtt acgacagcta caagttgctg ggagattcct
    10381 acgccatcgc gggcgactac aagaatgcag tcgccgcgta cggcaaggcg gcgcccgatg
    10441 ccaagaccgg cgaggtcgat ttcctgcgtg gccagatgct cgagcaactc ggccgccaca
    10501 aggaagcgcg cgacgcgctc aagcaggcgg tcgcgaaggg caacttcaag cgcatcggcg
    10561 cagcgtatct caccctgggc aatgccgaac tggccttgaa ggacaaggcg gcggcgcgcg
    10621 cagcctacga acaggccgag caggatccgg aaacgcgcgc cgacgcgcag cgcaccctca
    10681 agcaattggg gcacaagtga tccggcacgg acacgacaag caacttgacc acaaatattg
    10741 gctcttgcca ctaggcaaag tcgtcacgct ggtgtaacgt ttcagacttt tccgccgcct
    10801 tgactgcgga agccgctccg gaccggaacg gcgactgcag tggggccctc aaggtcagcg
    10861 actggttcaa tacacgatgg cacgtacgtc ggctcgagac cgcaaacggt tcaactggtg
    10921 gcgcacgatt gcgattgcat tcgcgattgc cttgcacgcc gg
//
LOCUS       DASZZC010000735         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_385266,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000735 DASZZC010000000
VERSION     DASZZC010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385266"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VHE37_15695"
     CDS             complement(<1..486)
                     /locus_tag="VHE37_15695"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HVT37033.1"
                     /translation="MRARARYGLAILLGAITGLALVKPVLTHEIAAQAISVGPWQTLR
                     GTGSASANPYVRSAVAVAGLYALSAQETIYFTAYRDSAGDALDGRCRYRVSGAAPRAR
                     WWSFTLYGDDNYLVDNPQHIYSVPMSRLVPDAAGAIAFEVGGAAQPAGWLPAPASGAY
                     SL"
     gene            complement(483..1394)
                     /locus_tag="VHE37_15700"
     CDS             complement(483..1394)
                     /locus_tag="VHE37_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVT37034.1"
                     /translation="MDTLSLILHDIRLDGAVFREAVLGAPWALAMHTPGVTSFHIVAR
                     GQAWLLRDVADPLQLQTGEIVLLPGGIRHRLVDDPQTQVEAIDVTPELGLLRREPLTA
                     GGSGASAHLVSGSFRFDAKLARPLVTALPEVIHLRSAGATPPPWLRIGLQFIAEETGK
                     PEPGQQVILNRVADILLVEALRIHLAALPEGTGSWLLALRDNALSSALAAMHRNPEKD
                     WTVPELAELAHLSRSAFADRFAQVMGQPPLSYLTEHRMRLAASKLALGASVAQVAAQV
                     GYASETAFSQAYKRHHGHPPSKARGGA"
     gene            1639..2547
                     /locus_tag="VHE37_15705"
                     /pseudo
     CDS             1639..2547
                     /locus_tag="VHE37_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
     gene            2749..2940
                     /locus_tag="VHE37_15710"
     CDS             2749..2940
                     /locus_tag="VHE37_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004990351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HVT37035.1"
                     /translation="MSRATAADAQRYQCTDCGYVYDEAQGCVREGFAPGTRWSQIPQD
                     WACPDCAVREKPDFVAVKN"
BASE COUNT          516 a         1053 c         1044 g          490 t
ORIGIN      
        1 gagcgagtac gcgccgctgg ccggcgccgg cagccagccg gcgggctgcg ccgcgccgcc
       61 gacttcgaag gcaatcgcgc ccgccgcatc gggcaccagt cggctcatcg gcactgaata
      121 aatgtgctgc ggattgtcga ccaggtagtt gtcgtcgccg tagagcgtga agctccacca
      181 gcgcgcgcgc ggcgcggcgc cgctcacgcg gtagcggcag cggccatcga gcgcatcgcc
      241 ggcgctgtcg cggtaggcgg tgaagtagat cgtctcctgc gccgacagcg cgtagagacc
      301 agccaccgcc acggcgctgc gcacgtaggg attggccgag gccgagccgg tgccgcgcag
      361 cgtctgccaa ggcccgacgc tgatcgcctg cgccgcgatc tcgtgggtga gcaccggctt
      421 gaccagtgcc agcccggtga tggcgccgag caatatcgcg agaccgtaac gcgcgcgcgc
      481 tctcatgcgc cgccgcgcgc cttcgacggc ggatgcccgt gatggcgctt gtacgcctgc
      541 gagaacgccg tctccgacgc atagcccacc tgcgccgcca cctgcgccac gctcgcgccc
      601 agcgccagct tcgatgccgc cagccgcatg cggtgctcgg tgagatacga cagcggcggc
      661 tggcccatca cttgcgcgaa gcggtcggcg aaggcgctgc gcgaaagatg cgccagctcg
      721 gcgagttcgg gcaccgtcca gtccttttcc gggttgcggt gcatggcggc cagcgcgctg
      781 gacagcgcgt tgtcgcgcag cgccagcagc cagctgccgg tgccttcggg cagcgccgcg
      841 agatggatgc gcagcgcctc caccagcagg atgtcggcca cgcggttgag gatcacctgc
      901 tgtccgggtt cgggcttgcc ggtttcctcg gcaatgaact gcaggccgat gcgcagccat
      961 ggcggcggcg tggcgccggc gctgcgcagg tggatcacct ccggcagcgc ggtcaccagc
     1021 gggcgcgcca gcttggcgtc gaagcggaaa ctgccggaca ccaggtgcgc gctggcgccg
     1081 ctgccgccag cggtgagcgg ctcgcgccgc agcaggccga gttccggcgt cacgtcgatc
     1141 gcttcgacct gcgtctgcgg atcgtcgacc agccggtggc ggatgccgcc gggcagcagc
     1201 acgatctcgc cggtctgcag ctgcagcgga tcagcgacgt cgcgcagcag ccaggcctgg
     1261 ccgcgcgcca cgatgtggaa actggtgacg cccggcgtgt gcatggccag cgcccagggc
     1321 gcgccgagca cggcttcgcg gaacaccgcg ccgtcgaggc ggatgtcgtg caggatcaga
     1381 cttagggtgt ccactcggcg tttcgcatag ctggtccggc gtttgccgaa ggtggtggcg
     1441 ttttgcgact gttttgcggc ggcatgcgca ttcccctgcg ccggcgcaga atcgactata
     1501 gcaccgcctt ttgcgagacc tggacggagc acaacgatga acaccgcaac actgagcacc
     1561 accgccgcca ccaccgagaa atgggtcgac ggcaagcgct atctctggct gctgtcgccg
     1621 gccatcccgc tgctggccat gatcggcctg ggcatctacg aatacaccgg gcagccggcc
     1681 ttcgcctggt ccggtccgct gctgctgtac gtggtgatcc cggtgctcga ctggctgatc
     1741 ggcgtggacc gcaacaatgc gccggagtcg gcggtcgagc agctggaaaa tgatccgtat
     1801 taccgcgcga tcgtttatgc ctacattccc gcgcagtacc tcgtcaccat ctggggcgcg
     1861 tggatcgccg cgcacgacca gctgtcggcg ttcgccttca tcggcctggt gttcaccgtc
     1921 ggcgtgatca acggcatcgc catcaacacc gcgcacgagc tgggccacaa gaagggctcg
     1981 ctggagcgct ggctggccaa gatcacgctg gcgccggtgg cctacggaca tttctacgtg
     2041 gagcacacgc gcggccatca caagaacgtg gccacgcccg atgacccggc aagctcgaag
     2101 atgggcgaga ccttctgggc tttcctgccg cgcaccatga ttggcagcgc gcgctcggcc
     2161 tggaacatcg agaaggaaag actggcgcgc aacggcaagg gcgtgtggag caccgacaac
     2221 gacaacctgc agtcctgggc catgaccgtg gtgctgttcg gcgccatcac gctggccttc
     2281 ggcaccgctg cgctgccgtt cctgctgctg caggcgttct acggggcgag cctgctggaa
     2341 gtggtgaatt acctcgagca ctacggcctg ctgcgccaga agggcgccga cggccgctac
     2401 gagcgctgca agcctgagca ctcgtggaac agcaaccaca tcgtcaccaa tctctttctc
     2461 taccagctgc agcgccacag cgaccaccac gccaatccca cgcgccgctt ccaggctttg
     2521 cgccacttcg acgaaagccc gcagctgccc tcgggctacg cctcgctgat cctgatcgcc
     2581 tacgtgccgt ggctgtggtt ccggcagatg gacccggcgg tcgtgcgcca ttacaacggc
     2641 gacctgcgcc gcgccaatct ttatccgccc aagcgagcgc agctgctggc gcgctggagc
     2701 ggggtgaagc tcgacgacgg caatccgaag atcttccccg gcgagatcgt gtcgcgcgcc
     2761 actgcggcgg acgcgcagcg ctaccagtgc accgactgcg gctatgttta cgacgaagcg
     2821 cagggctgcg tgcgcgaagg cttcgcgccc ggcacgcgct ggtcgcagat tccgcaggac
     2881 tgggcctgcc cggactgcgc ggtgcgcgag aagccggact tcgtggcggt caagaactaa
     2941 ccagcttgca tgggtcaatc cgaacgaagt gaggaatctc accctgttga gattcctcgc
     3001 tacgcccgga atgaaaataa atgccgaaca tgaaatttaa aaatatcagt gcgccggcga
     3061 cacggtgtct tcgcgcagcg aagcgggtgc gggagccggc gca
//
LOCUS       DASZZC010000736         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1215423,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000736 DASZZC010000000
VERSION     DASZZC010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215423"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VHE37_15715"
     CDS             <1..1533
                     /locus_tag="VHE37_15715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVT37036.1"
                     /translation="GGGGSAPASPPYQPPPVPTPVAPLTRISADSPFTTTCGQTGGTL
                     YHNAEVEPWIAVNPSDATNLIAVWQQDRWSSGGAQGLLTGYSLDGGTTWSRTAPAFSA
                     CAGGSFPRASDPWITFSPNGTAFQIALVFSGDVRAAGSSSAMLVSRSRDGGASWDAPV
                     SLTSGQEGASVFNDKESITADPNNTLDVYAVWDQLDSNTHGATWFSRTLDGGDSWGAT
                     PTMIFDPGAHNQTIGNQIVVLPAGDLLLFFTEIDGIGTNSSSARFRLLRSTDDGATWP
                     AASAVTVMEELAVGARDPQTGALIRDAASLPQVKVSAAGDVYVVAQDARFSGGQRDGI
                     IVTVSHDGGLNWSSPVQLNGEPAVQAFVPSVAVRASDGLIGVTYYDLRHNTSDPSTLL
                     TDYWLALSSDGVSWNDNFLAGPFDLDLAPQANGLFVGDYQSLALQGEAFVPVFVQTTA
                     AAGDPTDVYEASYAPAAVMEKSRAQLRPMLAQAPAVSADFRARVQENLENLAGETRQP
                     PR"
     gene            complement(1539..1721)
                     /locus_tag="VHE37_15720"
     CDS             complement(1539..1721)
                     /locus_tag="VHE37_15720"
                     /inference="COORDINATES: protein motif:HMM:NF021321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HVT37037.1"
                     /translation="MNPELPAADLTLPPRPERPDCCGGGCAVCVLDSWYDELERWERE
                     CAEILRRREQAAQQQQ"
     gene            1801..>2110
                     /locus_tag="VHE37_15725"
     CDS             1801..>2110
                     /locus_tag="VHE37_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVT37038.1"
                     /translation="MSAAVGFAAFDPASPLREHKFVRRDPRADDVQIEILYCGVCHSD
                     LHTVRSEWSGTQYPCIPGHEIVGRVTKVGSEVTRYKIGDLVGVGCMVDSCRTCSQCKE
                     G"
BASE COUNT          331 a          744 c          702 g          333 t
ORIGIN      
        1 ggcggcggcg gttcggcgcc ggcgtcgccg ccgtaccagc cgccgccggt accgacgccg
       61 gtggcgccgc tcacgcgcat ctccgccgac tcgcccttca ccaccacctg cggccagacc
      121 ggcggcacgc tttaccacaa cgcggaagtg gaaccgtgga tcgcggtgaa tccatccgat
      181 gccaccaacc tcattgccgt gtggcagcag gaccgctggt ccagcggcgg cgcgcagggc
      241 ctgctcactg gctactcgct cgatggcggc accacctggt cgcgcaccgc gccggcattc
      301 tctgcctgcg ccggcggcag ctttccgcgc gcgtccgatc cgtggatcac gttttcgccg
      361 aacggcaccg cgttccagat cgcgctggtg ttttccggcg acgtgcgcgc ggcgggctcc
      421 agcagcgcca tgctggtcag ccgctcgcgc gacggcggcg ccagctggga cgcgccggtg
      481 tcgctgacca gcgggcagga gggcgccagc gtgttcaacg acaaggaaag catcaccgcc
      541 gatccgaaca acacgctgga tgtctacgcg gtgtgggatc agctcgacag caacacgcac
      601 ggcgccacct ggttctcacg cacgctcgac ggcggcgaca gctggggtgc aacgccaacc
      661 atgatcttcg accccggcgc ccacaaccag accatcggta accagatcgt ggtgctgccg
      721 gcgggcgacc tgctgctgtt tttcaccgag atcgacggca tcggcaccaa cagttccagc
      781 gcgcgcttcc gcctgctgcg ctccaccgat gacggcgcca cctggccggc ggcaagcgcg
      841 gtgacggtga tggaagaact cgcggtgggc gcgcgcgatc cgcagaccgg cgcgctgatc
      901 cgcgacgccg cctcgctgcc gcaagtgaag gtgagcgcag cgggcgatgt ttatgtggtg
      961 gcgcaggatg cgcgcttctc cggcggccag cgtgacggca tcatcgtcac cgtctcgcat
     1021 gacggcggac tgaactggag ttcgccggtg cagctcaacg gcgagccggc ggtacaggct
     1081 ttcgtgccca gcgtggcggt gcgggccagc gacgggctga tcggcgtcac ctactacgac
     1141 ctgcgccaca acacctccga tccctcgacg ctgctcaccg actactggct ggcgctgtca
     1201 tcggatggcg tcagctggaa cgataatttc ctcgccgggc cgttcgatct ggatctcgcg
     1261 ccgcaggcca atggcctatt cgtcggcgac taccagagcc tggcgctgca gggcgaggcc
     1321 ttcgtgccgg tgttcgtgca gaccacggcc gccgccggcg atcccaccga cgtctacgag
     1381 gccagctacg cgccggcggc ggtaatggag aaatcgcgcg cgcagctgcg cccgatgctg
     1441 gcgcaggcgc cggcggtatc ggcggatttc cgcgcgcgcg tgcaggagaa cctggaaaac
     1501 ctcgccggcg agacgcgcca gccgccgcgc tgaagctctt attgctgctg ctgcgccgcc
     1561 tgctcgcgcc ggcgcaggat ttccgcgcac tcgcgttccc agcgctcgag ctcgtcgtac
     1621 cagctgtcga gcacgcatac cgcgcagccg ccgccgcagc agtccggccg ctcggggcgc
     1681 ggcggcaatg taaggtcggc ggcgggcaac tcgggattca tgcagggctt gcaaggctga
     1741 acgcgacagg ccaaattctg tcctattcca gttcgacctt ccccttcaac ggagaactcc
     1801 atgtccgccg ccgtcggctt tgccgcgttt gatcccgctt cgccgctgcg cgagcacaag
     1861 ttcgtgcggc gcgatccgcg tgccgacgac gtgcagatcg agattctcta ctgcggcgtc
     1921 tgccacagcg acctgcacac cgtgcgcagc gagtggagcg gcacgcagta cccgtgcatc
     1981 cccggccatg agatcgtcgg ccgcgtcacc aaagtcggca gcgaggttac gcgctacaag
     2041 atcggggacc tggtgggcgt gggctgcatg gtcgacagct gccgcacctg ttcccaatgc
     2101 aaggagggac
//
LOCUS       DASZZC010000737         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_255998,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000737 DASZZC010000000
VERSION     DASZZC010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255998"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..981
                     /locus_tag="VHE37_15730"
     CDS             4..981
                     /locus_tag="VHE37_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVT37039.1"
                     /translation="MLIAAAVALPLTAAAGEVKLLNASYDPTRELYKDVNAKFAEAWK
                     AKTGDTVTIQQSHGGSGKQARAIIDGLEADVATLALGYDIDALYDKGRLIPQDWQKRL
                     PNNSSPYTSTIVFLVRKGNPKGIKDWADLAKPGVAVITPNPKTSGGARWNYLAAWAWA
                     QKQYGGDDAKTRDYIGRLYKNVPVLDSGARGSTTTFVQRGIGDVLISWENEAYLALKE
                     FGDGKFEIVAPSLSILAEPPVTVVDKYAQRHGTSKVAEAYLNFLYTDEGQEIVARNFY
                     RPTSPAVAAKYAAQFPKLNLVTIAEFGGWTKAQNTHFADGGVFDQIYQK"
     gene            1071..1289
                     /locus_tag="VHE37_15735"
     CDS             1071..1289
                     /locus_tag="VHE37_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HVT37040.1"
                     /translation="MSIAAINTRNQFKGHVVAIHRGPVVSEVEIETAAGIVGAIVTSS
                     SIDTLKLRLGDEALAVFKATEVLVAKLG"
     gene            1297..1920
                     /locus_tag="VHE37_15740"
     CDS             1297..1920
                     /locus_tag="VHE37_15740"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HVT37041.1"
                     /translation="MCLMIHKPRGAAIPADLLEAAVLLNRDGWGLMGFAPSGDLLLER
                     HAEVSLDDLLHAEECYRGAEYVLHLRRRTRGAANQDNSHPFKVGDGIYLMHNGTLRLD
                     STVPGMSDTWHLVSEILRPLSQRRPGLLLDHAFQRLLKLALTPENKLALLDHDARRIV
                     LLNQEHGAELDGLWLSSTRWIDRARLPLVTPPQAQERSYHAGELVFA"
     gene            1917..>2659
                     /gene="cysT"
                     /locus_tag="VHE37_15745"
     CDS             1917..>2659
                     /gene="cysT"
                     /locus_tag="VHE37_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HVT37042.1"
                     /translation="MSALTASRRTAIPGFGLSLGFTLTYLSLIVLIPLAGVFFKTAGL
                     GLDGVWEAITATRVLAALKLSFGAALLAALLNAVFGSLVAWVFVRYQFPGKRAFDAVI
                     DLPFALPTAVAGIALTALYAPNGWIGQFLEPHGIKVAYTPLGVIVAMTFIGLPFVVRT
                     VQPVLEEAEKELEEAAASLGATRAQTIARVILPSVLPALLTGFALAFARAVGEYGSVI
                     FIAGNLPFKSEIAPLLLIIKLEEFDYRGAT"
BASE COUNT          463 a          899 c          853 g          444 t
ORIGIN      
        1 cacatcctca tcgcagccgc cgtcgccctg ccgctcaccg cggcagccgg cgaggtcaag
       61 ctgctcaatg cctcctacga tccgacgcgc gaactgtaca aggacgtcaa cgcgaaattc
      121 gccgaggcgt ggaaggcgaa aaccggcgac accgtgacca tccagcagtc gcatggcggc
      181 tcgggcaaac aggcccgcgc catcatcgat ggactggaag ccgatgtggc cacgctggct
      241 ctgggctatg acatcgacgc gctgtacgac aagggccggc tgattccgca ggactggcag
      301 aagcgcctgc ccaacaactc ttcgccgtat acctcgacca tcgtcttcct ggtgcgcaag
      361 ggcaacccca agggcatcaa ggactgggct gacctggcca agcccggtgt ggcggtgatc
      421 acgcccaacc cgaagacctc gggcggtgcg cgctggaatt atctggccgc ctgggcctgg
      481 gcgcagaagc agtacggcgg cgacgatgcg aagacgcgcg actacatcgg ccggctgtac
      541 aagaacgtgc cggtgctgga ttccggcgca cgcggctcca ccaccacctt cgtccagcgc
      601 ggcatcggcg acgtgctgat ttcgtgggag aacgaggcct atctcgcact gaaggaattc
      661 ggcgacggca agttcgagat cgtggcgcca tcgctgtcga tcctggccga accgccggtg
      721 acggtggtcg acaagtacgc gcagcgccac ggcaccagca aggtggcgga ggcctacctg
      781 aacttcctct acaccgatga aggccaggag atcgtcgcca ggaacttcta ccgcccaacc
      841 agcccggcgg ttgcggcgaa atatgcggcg cagtttccca agctgaacct ggtcaccatt
      901 gccgagttcg gcggctggac caaggcacag aacactcatt tcgcagatgg cggagtgttc
      961 gaccagatct accaaaagta gaaattctcc cagtggtcat tccccgggca gcaatgcccg
     1021 gtcttttttg atacagcaat gcccggcctt tcttgaaaca ggaacatgca atgtccatcg
     1081 cagccatcaa cacccgcaac cagttcaagg gccacgtggt cgcgattcat cgcggcccgg
     1141 tggtgtcgga agtggaaatc gaaaccgcgg ccggcatagt cggcgccatc gtcacctcca
     1201 gctcgatcga cacgctgaag ctgcgccttg gcgacgaggc gctggcggtg ttcaaggcca
     1261 ccgaagtgct ggtggccaag ctcgggtgaa gcgaccatgt gcctgatgat ccacaagccg
     1321 cgcggcgcgg ccatcccggc cgacctgctc gaagcggcgg tgctgctgaa ccgcgacggc
     1381 tggggcctga tgggcttcgc gccttccggc gacctgctgc tcgaacgtca cgcggaagtc
     1441 tcgctcgacg acctgctgca tgccgaggag tgctaccgcg gcgcggaata tgtgctgcac
     1501 ctgcggcggc gcacgcgcgg cgcggccaac caggacaact cccatccgtt caaggtcggc
     1561 gacggcattt acctgatgca caacggcacg ctgcggctcg acagcaccgt gcccggcatg
     1621 tcggacacct ggcacctggt gagcgaaatc ctgcggccgc tgtcgcagcg gcggcccggc
     1681 ctgctgctcg atcacgcgtt ccagcgcctg ctcaagctgg cgctgacgcc ggagaacaag
     1741 ctcgcgctgc tcgatcatga cgcgcggcgc atcgtgctgc tcaaccagga gcatggcgcg
     1801 gagctggatg gcctgtggct gtcgagcacg cgctggatcg accgcgcgcg cctgccgctg
     1861 gtgacgccgc cgcaggcgca ggagcgcagc taccacgccg gcgaactggt gtttgcatga
     1921 gcgcactcac cgccagccgg cgcacggcca ttcccggctt cggcctcagc ctgggcttca
     1981 ccctcaccta cctgtcgctg atcgtgctga tcccgctggc gggcgtcttt ttcaagacgg
     2041 ccgggctcgg cctcgacggg gtgtgggagg cgatcaccgc cacacgcgtg ctggcggcgc
     2101 tcaaactctc cttcggcgca gcgctgctgg ctgcactgct caacgcggtg ttcggctcgc
     2161 tggtggcctg ggtcttcgtg cgctaccagt ttccgggcaa gcgcgcgttc gacgcggtga
     2221 tcgacctgcc gttcgccctg cccaccgccg tggccggcat cgcgctcacc gcgctgtacg
     2281 cgcccaacgg ctggatcggc cagttcctcg aaccgcatgg catcaaggtg gcgtacacgc
     2341 cgctgggcgt gatcgtggcc atgaccttca ttggcctgcc gttcgtggtg cgcaccgtgc
     2401 agccggtgct ggaagaggcg gagaaggaac tcgaggaggc cgcggcctcg ctcggcgcca
     2461 cccgcgcgca gaccattgcg cgcgtgatcc tgcccagcgt gctaccggcg ctgctcaccg
     2521 gattcgcgct ggctttcgcc cgggcggtcg gcgaatacgg ctcggtgatc ttcatcgccg
     2581 gcaacctgcc gttcaagtcg gagatcgcgc cgctgctgct catcatcaag cttgaggagt
     2641 tcgattaccg cggcgccac
//
LOCUS       DASZZC010000738         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_572338,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000738 DASZZC010000000
VERSION     DASZZC010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572338"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHE37_15750"
     CDS             <1..264
                     /locus_tag="VHE37_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37043.1"
                     /translation="MFAALIMINKAVSGLGVFLSGVILWAVGFPDKAEPATLDPAIVR
                     HLAWLFVLSVGGACTLAVVCLAYYPISREMHERILQQLRQRSS"
     gene            352..927
                     /locus_tag="VHE37_15755"
     CDS             352..927
                     /locus_tag="VHE37_15755"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HVT37044.1"
                     /translation="MLQIVPSSVGRTLSGLRAGLTEVVYLAPGVAEIPETISVTSAAF
                     GKTERLPQRYTADGEGLSPPLAWSNVPETTQAFLVLVEDADSPSPRPLVHAIAWGIAG
                     DVRGLPEAALNAVDGATRSCILGLNSTLRRSYLPPDPPPGHGDHQYLFQVYALDQAVE
                     FDEPPGRSAIVDTVRGLATAKGCLVGIYSRD"
     gene            1005..>1651
                     /locus_tag="VHE37_15760"
     CDS             1005..>1651
                     /locus_tag="VHE37_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT37045.1"
                     /translation="MSKVRDFLPPGDFSALAFIPYDLVSLLALGMALSFIVADRNSLT
                     SRYLALAFVFIGLSIDLNIVIGIQFNVRAALHGWFAAGEALASIALLEWIMRVRRTVP
                     AGDLDVRAGDRVLRVGQLCAAAYFALAVKFPEIRLNDFLGALNHQQPFARPGFWLFAA
                     PMFFSGAAGTFSILLLLNRHPDPPEKARVAAMALAIPFLMSSFVLPLFYNALPLML"
BASE COUNT          221 a          587 c          548 g          295 t
ORIGIN      
        1 atgtttgccg cgctgatcat gatcaacaag gcggtctcgg gcctgggcgt gttcctgtcc
       61 ggcgtcatcc tgtgggcggt cggctttccc gacaaggctg agcccgccac gctggacccg
      121 gccatcgtca ggcacctggc ctggctgttc gtgctgtcgg tgggtggcgc ctgcacgctg
      181 gcggtggtct gcctggcgta ttacccgatc tcgcgcgaga tgcacgagcg catcctgcag
      241 caactcaggc agcgcagcag ctgacatatc tcggtgcccg ccgatgcgcc gcgccgaccg
      301 aacaaatcgc cgcgcgcgcc ggtctcacgc aggagttcct gcggaggctg catgctgcaa
      361 atcgttccat cgagcgtcgg gcgcacgctc agcgggctgc gcgccgggct gaccgaggtg
      421 gtgtacctgg cgccgggcgt ggcggaaatc ccggaaacca tcagcgtgac cagcgcagcc
      481 ttcggcaaga ccgaacgcct gccgcagcgt tacaccgctg acggcgaagg gttgtcgccg
      541 ccgctggcct ggagcaacgt gccggaaacc acgcaggcct tcctcgtact ggtggaagac
      601 gccgacagtc cgtcgccgcg cccgctggtg cacgccatcg cctggggcat cgccggcgac
      661 gtgcgcggcc tgcccgaggc tgcgctcaac gcggtcgatg gcgccacgcg cagctgcatc
      721 ctcggcctca attccaccct gcggcgcagc tacctgccgc ccgatccgcc gccgggccac
      781 ggcgaccacc agtacctgtt ccaggtgtac gcgctggacc aggcggtgga gttcgacgag
      841 ccgccgggac gcagcgcgat cgtcgacacc gtccgcgggc tggctaccgc caagggctgc
      901 ctggtgggga tctattcgcg cgactgagtg cgcgtgcggc gcgccgcgca aagcattaac
      961 ctgcgcaggg cgccgcccgt cgcgcggcgc ggagtgcatc gacggtgtcc aaggtccgcg
     1021 actttcttcc gcccggcgat ttctccgcgc tggccttcat cccgtatgac ctggtcagcc
     1081 tgctggcgct gggcatggcg ctgtcgttca tcgtcgccga ccgcaactcg ctgaccagcc
     1141 gttatctcgc attggccttc gtcttcatcg gcctgtccat cgacctcaat atcgtcattg
     1201 gcatccagtt caacgtgcgc gccgcgctgc acggctggtt cgcggcgggc gaggcgctgg
     1261 ccagcatcgc gctgctggaa tggatcatgc gcgtgcgccg cacggtgccg gcgggcgatc
     1321 tcgacgtgcg cgccggcgac cgcgtgctgc gcgtgggcca gctatgcgcg gcggcgtatt
     1381 tcgcgctggc ggtgaagttc ccggaaatcc gcctcaacga ttttctcggc gcgctcaacc
     1441 accagcagcc gtttgcgcgg ccgggcttct ggctgttcgc cgcgccgatg ttcttcagcg
     1501 gcgccgccgg caccttctcc atcctgctgc tgctcaaccg ccatcccgat ccgccggaga
     1561 aggcgcgcgt ggcggcgatg gcgctggcca tcccgttcct catgtccagc ttcgtgctgc
     1621 cgctgttcta caacgcgctg ccgctgatgc t
//
LOCUS       DASZZC010000739         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_117675,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000739 DASZZC010000000
VERSION     DASZZC010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117675"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VHE37_15765"
     CDS             <1..289
                     /locus_tag="VHE37_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HVT37046.1"
                     /translation="EAMKLLDVDTNGFDLMDRKLLLTLIERFDGGPAGVDNLAAAIGE
                     ARDTIEDVIEPYLIQQGYLMRTPRGRVAGKLAYQHYGLKAPDKLNTPDLFS"
     gene            292..687
                     /gene="ybgC"
                     /locus_tag="VHE37_15770"
     CDS             292..687
                     /gene="ybgC"
                     /locus_tag="VHE37_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HVT37047.1"
                     /translation="MSAESIFRLRVYYEDTDVSGVVYHANYLRWFERARTEWLRALAL
                     GQEELRVTEGVAFTVANLSIDYLKPARLDDELQVMTVISEMGRASLGFSQRVQRAGEL
                     LARAQVRVGCVDAASFKPRRLPAGVKWNA"
     gene            842..1525
                     /gene="tolQ"
                     /locus_tag="VHE37_15775"
     CDS             842..1525
                     /gene="tolQ"
                     /locus_tag="VHE37_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HVT37048.1"
                     /translation="MNSNMSFANLVMHASALAQVVLLILLAAAIVSWWVILAKRKFLR
                     NTSDAADRFEEKFWSGGNLADLYESIRRDQHAQGIAAIFSAGYEEYSRQQTAGRIDPD
                     DSIAAIQRQMRVSQVREVERLETGLSWLATIGSTSPYVGLFGTVWGIMNAFIGIGNVK
                     QASLAVVAPGIAEALIATAMGLFAAIPAVIAYNFFTRGVERLENRFHTFADEMIGIVE
                     RGLRHAQKS"
     gene            1534..1953
                     /gene="tolR"
                     /locus_tag="VHE37_15780"
     CDS             1534..1953
                     /gene="tolR"
                     /locus_tag="VHE37_15780"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HVT37049.1"
                     /translation="MARRKLSSEINVVPYIDVMLVLLIIFMITAPLLTQGIAVDLPKV
                     DAANISTSQEPTTVSVSAEGQFYINKSDDPHRAVDEPELAAIVKKLVEAKPDDLILVE
                     ADGQAHYEDVAKAMAILQTSGATKVGFVTKPADKKDL"
     gene            1975..2925
                     /gene="tolA"
                     /locus_tag="VHE37_15785"
     CDS             1975..2925
                     /gene="tolA"
                     /locus_tag="VHE37_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HVT37050.1"
                     /translation="MDFRLRYLVYSILLHVLLFVLLIASSFFTRPIEIEPVMTAVLVS
                     PPPRPKPPPPKPPEPKPEPPKPEPPKPKPEPPKPPEPTPEQRVADVLRKLDCDTIPDL
                     KAEAGRRIAVEAAVIYKQIALMEAKCKKKEEDKKKQDEAAEKKRLEQEALKEKQEQER
                     QKKEEEKRQKEEDLQRQKDLQQQLAEEQRQREEAERAAQQAALEAAAAAEANARADAL
                     ADKAIQEWAGRLKEKVRRYWIQPPNMSDSLVAKVRISWLPSGTVTKVSIVQSSGNAGY
                     DDSVQRAILKSDPLPALNDARAYDKARDLILNFPAKELTH"
BASE COUNT          596 a         1000 c          970 g          482 t
ORIGIN      
        1 ggaggcgatg aagctgctcg acgtcgacac caatggcttc gacctgatgg accgcaagct
       61 gctgctcacg ctgatcgagc gtttcgacgg cggcccggcc ggcgtcgaca acctggcggc
      121 ggcgatcggc gaggcgcgcg acaccatcga ggacgtgatc gagccgtacc tgatccagca
      181 gggctacctg atgcgcacgc cacgcgggcg cgtggccggc aagctggcgt accagcacta
      241 cggcctgaag gcgccggaca agctcaacac gccggacctg ttctcctgag catgagcgcg
      301 gaatctattt tccgcctgcg tgtctactac gaggacacgg atgtcagcgg cgtggtctat
      361 cacgccaact acctgcgctg gttcgagcgc gcgcgcaccg agtggctgcg cgcgctggcg
      421 ctggggcagg aagaattgcg ggtgaccgag ggcgtggcgt tcaccgtggc gaatctttcg
      481 atcgattacc tgaagccggc gcggctggac gacgagctgc aagtgatgac ggtgatcagc
      541 gagatgggcc gcgccagcct cgggttcagc cagcgcgtgc agcgcgccgg cgaactgctg
      601 gcgcgcgcgc aggtgcgcgt gggttgtgtc gatgcggcga gcttcaaacc gcggcggctg
      661 cccgctggcg taaaatggaa cgcctagcgc gcccgttttt ggctgcaccg caccccggat
      721 tttgatgtta ctatttatta tctgaaagta atttcaacct attttttata acagctgcaa
      781 gacgggtgcg gtggacctgc cggcggctat gcagaacaag aaatcgtccc gaggattccg
      841 catgaactcc aacatgtcct tcgccaacct ggtgatgcac gcaagcgcgc tggcgcaggt
      901 ggtgctgctg atcctgctgg ccgccgccat cgtctcctgg tgggtgatcc tggccaagcg
      961 caagttcctg cgcaatacca gcgacgcggc cgaccgtttc gaggaaaaat tctggagcgg
     1021 cggcaacctg gccgacctgt atgaatcgat ccgccgcgac cagcacgcac agggcatcgc
     1081 cgccatcttc tccgccggct acgaggagta ttcgcgccag cagaccgcgg gccgcatcga
     1141 cccggatgat tccatcgcgg cgatccagcg ccagatgcgt gtttcgcagg tgcgcgaagt
     1201 ggagcggctg gagacgggcc tgtcgtggct ggccaccatc ggctccacca gtccgtacgt
     1261 gggcctgttc ggcaccgtgt ggggcatcat gaacgcgttc atcggcatcg gtaacgtgaa
     1321 gcaggcctcg ctcgctgttg tcgcgcccgg catcgcagag gcgctgatcg ccaccgccat
     1381 gggcctgttc gccgccattc ccgcggtcat cgcctacaac ttcttcaccc gcggcgtgga
     1441 gcgcctggaa aaccgattcc acacctttgc cgacgagatg atcggcatcg tcgaacgcgg
     1501 cctgcgccac gcgcagaaaa gctgagagcc gccgtggccc gccgcaagct gtcgtccgag
     1561 atcaacgtcg ttccgtatat cgacgtgatg ctggtgctgc tgatcatctt catgatcacc
     1621 gcgccgttgc tcacccaggg catcgcggtc gatctgccca aggtggacgc cgccaacatc
     1681 tccacctcgc aggagccgac cacggtgtcg gtgtcggccg aagggcagtt ctacatcaac
     1741 aagtccgacg atccgcaccg cgcggtcgat gagcccgagc ttgcagccat cgtcaagaag
     1801 ctggtggagg ccaagcccga cgacctgatc ctggtcgagg ccgacggcca ggcgcactac
     1861 gaagacgtgg ccaaggccat ggcgatcctg cagacctcgg gcgccaccaa ggtcggcttc
     1921 gtcaccaagc ccgctgacaa gaaagacctc taagccgcca gcccagccgc cgcagtggat
     1981 ttccgcctcc gctatctggt ctattcgatc ctgctgcacg tgctgctgtt cgtgctgctg
     2041 atcgccagct cgttcttcac ccgcccgatc gagatcgagc cggtgatgac cgcggtgctg
     2101 gtgtcgccgc cgcccaggcc caagccgccg ccgcccaagc cgccggagcc caagcccgag
     2161 ccgcccaagc cggaaccgcc gaaaccgaag cctgagccgc ccaagccgcc ggagcccacg
     2221 cccgagcagc gcgtggccga cgtgctgcgc aagctcgact gcgacaccat tcccgacctc
     2281 aaggccgagg ccggccgccg catcgcggtg gaagccgccg tgatctacaa gcagatcgcg
     2341 ctgatggagg ccaagtgcaa gaagaaggaa gaggacaaga agaagcagga tgaggccgcc
     2401 gagaagaagc gcctggaaca ggaagcgctg aaggaaaagc aggagcagga gcggcagaag
     2461 aaggaagaag agaagcggca gaaggaagaa gacctgcaac ggcagaagga cctgcagcag
     2521 cagctggcgg aggaacagcg ccagcgcgaa gaggccgagc gcgccgcgca gcaggccgcg
     2581 ctggaagccg ccgccgccgc cgaggccaat gcgcgcgccg acgcgctggc cgacaaggcc
     2641 atccaggaat gggccggccg gctgaaggaa aaggtgcgcc gctactggat ccagccgccc
     2701 aacatgtccg acagcctggt ggccaaggtg cgcatttcct ggctgcccag tggcaccgtg
     2761 accaaggtca gcatcgtgca gtcgtcgggc aacgccggct acgacgactc ggtgcagcgc
     2821 gccatcctca agtccgaccc gctgccggcg ctcaacgatg cgcgggccta cgacaaggcg
     2881 cgcgacctca tcctcaattt cccggcgaag gaactcacac actgattccg cggtctgtga
     2941 aaaaccctag tgcccgacga tccgtgcctg ccagtttttt catgccatca ggcataattc
     3001 gcgtcccgct gtgagcaccc ccatgaaacg tcttcttgca ttcatgct
//
LOCUS       DASZZC010000740         7129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_317476,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000740 DASZZC010000000
VERSION     DASZZC010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317476"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VHE37_15790"
     CDS             <1..1056
                     /locus_tag="VHE37_15790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT37051.1"
                     /translation="ITDAWSWQWLFLINLAPGIAVAAAVWTLLDIDRPEHKLWRILDL
                     PGTVYMMLFLGSLEWVLEEGPAHNWLDDGGIAFWTATMCVSALLFFHRVLTHHNPIVS
                     LRPFINPHFIAANAAAMLLSVSLFAANYIVPLFLGQVRGFNSMQIGDALAVNGIAMFL
                     AAPFAARLARKFDLRYLFTFGCLLVLAGSAAMCTLTDQTGYWELFWPQGVRSLGFMLA
                     LVCCSTLSLSTLPPDMIKNASGLYTLMRNLGGALGLACLNTIMFWRGAVHQQHLSEAL
                     RLSRVPVRDLALQAGAQAPHDAILSVALVAHRLGVQSLVLAYNDALIAITWVGAIAIP
                     VFLFVKRPRLTGGPAMH"
     gene            1117..2886
                     /locus_tag="VHE37_15795"
     CDS             1117..2886
                     /locus_tag="VHE37_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT37052.1"
                     /translation="MSSTTSEAQAPRFSDLDLPEPLLRALADVGYETPTPIQAETIPA
                     LLEGRDLLGQAQTGTGKTAAFALPVLAKIDTRKSATQALVLAPTRELAIQVAEAFQKY
                     AAHLPGFHVLPVYGGQSYGPQLSALRRGAHVVVGTPGRVIDHMDRGTLQLNALKALVL
                     DEADEMLRMGFIDDVEKILGQTPPQRQVALFSATMPAPIRRIAQKHLKMPAEITIRAT
                     TTTAKNIRQRYWVADGASKLEALTRILEAEPFDGMIVFVRTKQATTELAERLVARGFA
                     VAPLSGDVEQKQRERTVARLKKSELDIIVATDVAARGLDVERISHVINYDIPHDTEAY
                     VHRIGRTGRAGRKGEAILFVAPRERNMLRIIERATRQHITPMELPSAEVVNDKRIAKF
                     MEGITQALGDETTREARRELQELIERYQREHPVSATEIAAALALAAPAAAVLVQSPAP
                     ALAPAREARTPAHERERPSRERKPRVGGAEAGMETFRVEVGRQHGVKPGNLVGAIANE
                     AGLDGSDIGRIEIFDDYSHVDLPEGMPKDTFRDLQKVWVVNQQLRISRVNGPNPPPPK
                     KFMPPKKTHSHQPARKGHPKHKG"
     gene            complement(2950..3654)
                     /locus_tag="VHE37_15800"
     CDS             complement(2950..3654)
                     /locus_tag="VHE37_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVT37053.1"
                     /translation="MRLNKYISETGVCSRREADAWIEAGRVLVNGRRAELGTQVNEGD
                     EVIANGRRIGARQQKHVYLALNKPPGITCTTERHIEGNIVDFIGHRERIFPIGRLDKD
                     SEGLILLTSNGDIVNEILRSENNHEKEYIVSVDQPFDDHFLARMARGVRLPELEQTTK
                     PCRVERMTAQTFRIVLTQGLNRQIRRMCEVHGLKVTRLKRVRIINVKLGALAVGKWRD
                     LAPDELHGLLPQRTQW"
     gene            3720..4883
                     /locus_tag="VHE37_15805"
     CDS             3720..4883
                     /locus_tag="VHE37_15805"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVT37054.1"
                     /translation="MTTTRLWCLCAAWLAGCGSGTPLQTQGQLADQSAPAIALRQAYG
                     GQTFDGALFLTHAPGDAQHVYVVQQSGVIWMLDNDDAGSNAHVFADLSAEVTDHGGEQ
                     GLLGLAFDPDYAGNGRFYVNYIPAGGTLRTVIAQLTAAADRQTADPGSEVQLLTYDQP
                     YGNHKGGWLGFGPDGLLYISAGDGGGTGDPDNHAQNLASPLGKILRIAADGSIPPDNP
                     FVSTPGARGEIYAYGLRNPFRASFDRSTGELWAGDAGQSAWEEIDRITAGGNYGWRKF
                     EGNHVYNADDPAPANALAPVFEYGHDSGRCVVIGGYVYRGNALTSLAGRYIFGDYCSG
                     EIFALDPAGNAAAADVIALAPARLTSFGEDGAGELYVTLADGRILRIAPGSGA"
     gene            complement(4887..5426)
                     /locus_tag="VHE37_15810"
     CDS             complement(4887..5426)
                     /locus_tag="VHE37_15810"
                     /inference="COORDINATES: protein motif:HMM:NF019440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HVT37055.1"
                     /translation="MNSFPRLAVWIKPAGRARERLRAAALRARVELGGPTFDEHLTLL
                     SGIEQPQEQAQRALVQLAGSVRPFAVRLGRCAHGRERMRSLYLRVLHSPELDDARRAA
                     EKCFATGGDSFDPHISLVYGSSDDAAKQRLCAQLEAGLADLSFTVESLHLVSASSAVP
                     VQEWRELAEKTLRPESSAE"
     gene            complement(5558..6157)
                     /locus_tag="VHE37_15815"
     CDS             complement(5558..6157)
                     /locus_tag="VHE37_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37056.1"
                     /translation="MAAAREDGTISGAIWIRPGGAAAAELQAVIDELQQRCGGPGGTP
                     HLALLSGIHTSAGDGQRKLSRLATRVRPFEVELGDIDFGDDYYRCLYVRALPSRELEA
                     ARRAALEVFGAPMQAGDGWHVPLLYGEESLDTRRALAREFGSRVRRSFAVDSLELVNA
                     ARGVPVSQWHGIARARLRDDAQRRQRTLGGVPYSQVGGG"
     gene            6322..>7129
                     /locus_tag="VHE37_15820"
     CDS             6322..>7129
                     /locus_tag="VHE37_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37057.1"
                     /translation="MNLRACLRLAALLAAALSSACANTAVHRPVADDMRSKIQNSEEV
                     IGIKQDALYATYIPSMMSAASARTCSVIPGLGGLLAGACAAAMEGPDAVINASRAKDA
                     AAAVAPLKDALADYSFDAQLRAELASSLSRNSAVHPVPEAISVTRQVSDADYEKTLRA
                     CHASALMFTTVDYHLSPDFSTLTVSSQSLIYPCSAKLRAAAGQPDLAALPADFQPLDV
                     ANAIYRNMIAYEARLPDASTASADNIARWHSGDAALLKAVLNQAAHELATQ"
BASE COUNT         1167 a         2513 c         2322 g         1127 t
ORIGIN      
        1 atcaccgacg cgtggtcgtg gcagtggctg ttcctgatca acctcgcgcc gggcatcgcc
       61 gtggcggccg cggtgtggac gctgctcgat atcgaccggc ccgagcacaa gctgtggcgc
      121 atcctcgacc tgcccggcac tgtctacatg atgctgttcc tgggttcgct cgaatgggtg
      181 ctggaggaag gcccggcgca caactggctc gacgacggcg gcatcgcctt ctggaccgcc
      241 accatgtgcg tgtcggcgct gctgttcttc caccgcgtgc tcacgcacca caatcccatc
      301 gtcagcctgc ggccgttcat caacccgcat ttcatcgccg ccaacgcggc ggccatgctg
      361 ctgtcggtat cgctgttcgc cgccaactac atcgtgccgc tgttcctggg ccaggtgcgc
      421 ggcttcaaca gcatgcagat cggcgatgcg ctggcggtaa acggcatcgc catgttcctg
      481 gccgcgccgt tcgctgcgcg gctcgcgcgc aagttcgacc tgcgctatct cttcaccttc
      541 ggctgcctgc tggtgctggc gggcagcgcc gccatgtgca cgctcaccga ccagaccggc
      601 tactgggaac tgttctggcc gcagggcgtg cgcagcctgg gcttcatgct ggcgctggtg
      661 tgctgctcca ccttgagcct gtcgacgctg ccgcccgaca tgatcaagaa cgccagcggc
      721 ctgtatacgc tgatgcgcaa cctcggcggc gcgctcgggc tggcctgcct caataccatc
      781 atgttctggc gcggtgcggt gcatcagcag cacctgagcg aagcgctgcg gctgtcgcgc
      841 gtgccggtgc gcgacctcgc gctgcaggcc ggcgcgcagg cgccgcacga cgcgatattg
      901 tcggtggcgc tggtcgcgca tcgcctgggc gtgcaaagcc tggtgctggc ctacaacgac
      961 gcgctgatcg ccatcacctg ggtgggcgcc atcgccattc cggttttcct gttcgtcaaa
     1021 cgcccgcggt tgacgggcgg gcccgcgatg cactaattcc gcgggaacgg cacgcgcgcg
     1081 gagtaccatg gcgcattaga gccaggaacc tccgtcatgt cgtccaccac ttccgaggct
     1141 caggccccgc gtttttccga tctggacctg cccgagccgc tgctgcgcgc attggccgat
     1201 gtgggctacg aaacgccgac gccgatccag gccgagacca ttcccgcgct gctcgaaggc
     1261 cgcgacctgc tcggccaggc gcagaccggc accggcaaga ccgccgcgtt cgccttgccc
     1321 gtcctggcga agatcgacac gcgcaagtcc gcgacgcagg cgctggtgct cgcacccacg
     1381 cgcgagctgg cgatccaggt tgcggaggcg ttccagaaat acgccgcgca cctgcccggc
     1441 ttccacgtgc tgccggtcta cggcggccag agctacggcc cgcagctgtc ggcactgcga
     1501 cgcggcgcgc acgtggtggt cggcaccccc ggccgcgtca tcgaccacat ggatcgcggc
     1561 acgctgcagc tcaacgcgct caaggcgctg gtgctggacg aagccgacga gatgctgcgc
     1621 atgggcttca tcgacgacgt ggaaaaaatc ctggggcaga cgccgccgca gcggcaggtg
     1681 gcgttgttct ccgccaccat gccggcgccg atccgccgca tcgcgcagaa gcacctgaag
     1741 atgcccgcgg agatcaccat ccgcgccacc accaccacgg cgaaaaacat ccgccagcgc
     1801 tactgggtgg cggacggcgc gagcaagctc gaagccctga cgcgcatcct cgaagccgaa
     1861 cccttcgacg gcatgatcgt gttcgtgcgc accaagcagg cgaccaccga actggcggaa
     1921 cggctggtgg cgcgcggctt cgccgtggcg ccactgtcgg gcgacgtcga gcagaaacag
     1981 cgcgagcgca cggtggcgcg gctgaaaaaa agcgaactcg acatcatcgt cgccaccgac
     2041 gtggctgcgc gcgggctcga cgtcgagcgc atcagccacg tgatcaacta cgacatcccg
     2101 cacgacaccg aggcgtatgt gcaccgcatc ggccgcaccg gccgcgccgg ccgcaagggc
     2161 gaggccatcc tgttcgtggc gccgcgcgaa cgcaacatgc tgcgcatcat cgagagggcc
     2221 acgcgccagc acatcacgcc gatggagctg cccagcgccg aggtggtcaa cgacaagcgc
     2281 atcgccaagt tcatggaagg catcacgcag gcactgggcg atgaaacaac gcgcgaggcg
     2341 cggcgcgaac tgcaggaact gatcgaacgc taccagcgcg agcacccggt atccgcaacg
     2401 gaaatcgccg ccgcgctggc gctggctgcg ccggccgctg cggtgctggt gcagagcccc
     2461 gcgccggcgc tcgcgcccgc gcgcgaggcg cgcactccgg cgcacgaacg cgaacgcccg
     2521 tcacgcgagc gcaagccgcg cgtgggcggc gccgaagccg gcatggaaac cttccgcgtc
     2581 gaagtcggcc gccagcacgg ggtcaagccc ggcaacctgg tcggcgccat cgccaacgaa
     2641 gccggcctcg acggcagcga catcggccgc atcgagatct tcgacgacta cagccacgtc
     2701 gatctgcccg agggcatgcc caaggacacc ttccgcgacc tgcagaaggt gtgggtggtc
     2761 aaccagcagc tgaggatcag ccgcgtcaat ggtccgaatc cgccgccgcc gaaaaaattc
     2821 atgcccccca aaaaaactca ctcgcaccag ccggcgcgca aggggcatcc gaagcacaaa
     2881 ggctgagcgt cagcgccggc aggcccagcg cggccagcag caaccatgcg cgcgtcggcc
     2941 tgccgggttt caccactgcg tcctctgcgg cagcagcccg tgcagctcgt caggcgccag
     3001 gtcgcgccac ttgcccaccg ccagcgcacc gagctttacg ttgatgatgc gcacgcgctt
     3061 gagccgcgtg accttcagtc cgtgcacctc gcacatgcgg cggatctgcc ggttgaggcc
     3121 ctgggtgagc acgatgcgga aggtctgcgc cgtcatgcgc tcgacgcggc agggcttggt
     3181 ggtctgctcc agttccggca ggcgcacgcc gcgcgccatg cgcgcgagga agtggtcgtc
     3241 gaacggctgg tcgaccgaga cgatgtattc cttctcgtgg ttgttctcgc tgcgcaggat
     3301 ctcgttgacg atgtcgccgt tgctggtcag caggatcagc ccttcgctgt ccttgtccag
     3361 gcggccgatc gggaagatgc gctcgcggtg gccgatgaag tcgacgatgt tgccctcgat
     3421 gtgccgctcg gtggtgcagg tgatgcccgg cggcttgttc agcgcaaggt agacatgctt
     3481 ctgctggcgg gcgccgatgc gccggccgtt ggcgatcacc tcgtcgcctt cgttcacctg
     3541 cgtgcccagt tccgcgcgcc ggccgttgac cagcacgcgc ccggcctcga tccaggcatc
     3601 ggcctcgcgg cgcgagcaga cgccggtctc gcttatgtat ttgttcaggc gcatgcagaa
     3661 attgtaaaac cggcgcgccc tccccgttgt cgaagcagca ggctcaagga cggcgcctca
     3721 tgacaacgac aagactgtgg tgcctgtgcg ccgcctggct cgcgggttgc ggttcgggca
     3781 cgccgctgca gacgcagggg cagctggcag accagtccgc gccggccatc gcactcaggc
     3841 aggcctatgg cggacagacc ttcgacggcg cgctgtttct cacccacgcg ccgggcgatg
     3901 cgcagcatgt gtacgtggtg cagcagtccg gcgtcatctg gatgctggac aacgacgacg
     3961 ccggcagcaa cgcccatgtg ttcgccgacc tcagcgcgga agtcaccgac catggcggcg
     4021 agcaggggct gctcggcctc gccttcgatc ccgattacgc cggcaacggc cgtttctacg
     4081 tcaactacat cccggccggt ggcacgctgc gcacggtgat cgcgcagctc accgctgctg
     4141 ccgatcgcca gaccgccgac ccgggaagcg aagtccagtt gctcacctac gaccagccct
     4201 acggcaacca caagggcggc tggctcggct tcggccccga cggcctgctg tacatcagcg
     4261 ccggcgacgg cggcggcacc ggcgaccccg acaaccatgc gcagaacctg gcctcgccgc
     4321 tgggcaagat cctgcgcatc gcggcggacg gcagcatccc gccggacaat ccctttgtct
     4381 ccacgccggg cgcgcgcggc gagatctacg cctatggcct gcgcaatccg ttccgcgcca
     4441 gcttcgaccg ctccaccggc gagctgtggg ccggcgatgc ggggcagagc gcctgggaag
     4501 agatcgaccg gatcaccgcc ggcggcaatt acggctggcg caagttcgag ggcaaccacg
     4561 tctacaacgc cgacgatccc gcgccggcca acgcgctggc accggtgttc gagtacggcc
     4621 acgacagcgg ccgctgtgtg gtgattggcg gctacgtgta ccgaggcaac gcgctgacct
     4681 cgctcgcggg ccgctacatt ttcggcgact actgttccgg cgagatcttc gcgctggatc
     4741 ctgccggcaa cgcggccgcg gccgacgtga tcgcgctggc cccggcgcgg ctcacctcgt
     4801 tcggcgagga cggcgccggc gagctgtacg tgacgctggc ggatggccgc atcctgcgca
     4861 tcgcgcccgg cagcggagct tgacgctcac tcagccgatg actcgggccg cagggtcttc
     4921 tccgccagct cgcgccactc ctgcaccggc accgcgctcg acgcgctgac caggtgcagg
     4981 ctttccacgg tgaatgaaag atcggccagg cccgcttcca gttgcgcgca cagccgctgc
     5041 ttggcggcat cgtcgctgct gccgtagacc aggctgatgt gcggatcgaa gctgtcgccg
     5101 ccagtggcga aacatttttc cgccgcgcgg cgcgcgtcat ccagttccgg tgagtgcagc
     5161 acgcgcaggt agaggctgcg catgcgctcg cggccatggg cgcagcgccc gagccggacc
     5221 gcgaacggcc gcacgctgcc ggcgagctgc accagcgcgc gctgcgcctg ctcctgcggc
     5281 tgttcgatgc ccgacagcag cgtgaggtgt tcgtcaaagg tcggcccgcc gagttcgacg
     5341 cgcgcacgca atgccgcggc gcgcagacgt tcgcgcgcgc ggccggccgg cttgatccag
     5401 acggccaggc gcgggaagga attcacgtgg acacgcaccc gattttggct ataccgcaag
     5461 tcatgttcat gtgttatcaa atatagtatg tttatatttt tatacaattt ttactaacag
     5521 gcaattgcac tgtgcggtga ggcatctgca ggccgcttca accgccaccc acctgcgaat
     5581 agggcacccc gcccagcgtg cgctgccgcc gttgcgcgtc atcgcgcaag cgcgcgcggg
     5641 cgatgccgtg ccattgcgac accggcacgc cgcgcgcggc gttcaccagt tccaggctgt
     5701 cgaccgcgaa tgaccggcgc acgcggctgc cgaattcgcg cgcgagcgcc cggcgcgtgt
     5761 cgagcgattc ctcgccgtaa agcaacggta cgtgccagcc gtcgcccgcc tgcatcggcg
     5821 cgccgaacac ttccagcgcc gcgcggcgcg ccgcttccag ttcgcggctg ggcagtgcgc
     5881 gcacgtacag gcagcggtag tagtcgtcgc cgaaatcgat gtcgccgagt tccacttcga
     5941 acggccgcac gcgggtcgcc aggcggctga gcttgcgctg gccgtcgccc gcgctggtgt
     6001 ggatgccgga cagcagcgcc aggtgcggcg tgccgcccgg accgccgcag cgctgctgca
     6061 gttcgtcgat caccgcctgc aattccgccg cggccgcgcc gcccggccgt atccagatgg
     6121 cgccgctgat cgtgccgtcc tcgcgcgctg ctgccatggt ccgctcctgc ttttgcatat
     6181 agaccggcgc ggcggcaaaa actccggacc aactttccgc cggcggacga acatgagagc
     6241 cctaccgggt gttttccccg atgtgacgac cgcgcaacca ccggtacagt gcgcgaggga
     6301 tttcgtcttg ggggaatcaa tatgaacttg cgtgcctgct tgcgcctcgc agcgctgctt
     6361 gctgccgcct tgtcgtctgc ttgtgccaac acggcggtac accggccggt cgccgacgac
     6421 atgcgcagca agatccagaa cagcgaggaa gtgatcggca tcaagcagga cgcgctgtat
     6481 gccacgtaca tcccgtcgat gatgagcgcc gccagtgcgc gcacgtgcag cgtgattccc
     6541 ggcctcggag ggctgctggc aggagcctgc gccgctgcca tggagggacc cgacgcagtg
     6601 atcaacgcat cgcgcgccaa ggacgccgca gccgcagtag cgccgctgaa ggatgcgctc
     6661 gcggactaca gtttcgatgc gcagttgcgg gcggaacttg ccagctcgct gtcacgcaac
     6721 agcgcggtgc atcccgtgcc ggaggcgatc agcgtcacgc gccaggtctc cgacgccgac
     6781 tacgaaaaga ctctgcgcgc ctgccatgcc agcgcgctga tgttcaccac ggtcgactat
     6841 cacctgtcgc cggatttctc cacgctgacc gtgtcatcgc agagcctgat ctatccgtgc
     6901 tcggccaagc tgcgcgccgc cgccggccag cccgacctcg ctgcgcttcc ggcggacttc
     6961 caaccgctcg acgtggccaa cgcgatctac cggaatatga tcgcctacga agcccggctg
     7021 cccgatgcaa gcacagccag cgccgacaac atcgcccgct ggcattcagg cgacgcggca
     7081 ctgctcaagg ccgtgttgaa ccaggccgcg catgagctgg caacgcaac
//
LOCUS       DASZZC010000741         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_695202,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000741 DASZZC010000000
VERSION     DASZZC010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695202"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHE37_15825"
     CDS             <1..220
                     /locus_tag="VHE37_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37058.1"
                     /translation="DGWQAGLALVDGILARGELADYRLAHAARADLCRRLGRIDDARD
                     SYQRALSLTRQQAEQRFLQRRLDQLEKK"
     gene            295..765
                     /locus_tag="VHE37_15830"
     CDS             295..765
                     /locus_tag="VHE37_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT37059.1"
                     /translation="MSSRITPFLWFDGKAEEAAKLYCSIFPNSKITQVVRSTVDTPGT
                     PRGGVLTVGFTLNGLEYTAMNGGPMVSFNESVSFVVHCKTQEEIDHYWDRLTADGGAP
                     VACGWLKDKYGLRWQITPDLLLELIASKDSARAQRAMQAMMKMVKIDLKTLENA"
     gene            765..1187
                     /locus_tag="VHE37_15835"
     CDS             765..1187
                     /locus_tag="VHE37_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37060.1"
                     /translation="MAVAREIFINLPVKDLDKSVAFFRAVGLSLDPRFGDGMNCLVVG
                     DSIRAMLMSHEQFAAVMPGPLADVSRPAALRLSLENRAAIEALMEKALAAGGRRYEPA
                     QDHGFMYEDGFLDLDGHHWSLFHLDYAAFDRARREQAA"
     gene            1184..1549
                     /locus_tag="VHE37_15840"
     CDS             1184..1549
                     /locus_tag="VHE37_15840"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVT37061.1"
                     /translation="MKYLCLVYGEEDKIAAMNDLECLAFDRDARASGHCVASEALQPV
                     STASTVRVRQGRLTVHDGPFAETKEALAGFYLIEARDLNEAIQLAAKIPPAQVGCVEV
                     RPIRPIREQPRNTAAPSHS"
     gene            1561..1986
                     /locus_tag="VHE37_15845"
     CDS             1561..1986
                     /locus_tag="VHE37_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT37062.1"
                     /translation="MLKQLNPYIHFHRDCAEAIAFYERVLGGKVEMMMKVKDAPPSAQ
                     MKPSKENENLVLHVRLSVGSYQILASDWISDQPFQPMHGFSLSLEYPTVDEARKVYDA
                     LSVGAREIRMPLQGTFWAKAFAMFVDQYGTPWMISGAMQ"
BASE COUNT          405 a          687 c          698 g          364 t
ORIGIN      
        1 cgacggctgg caggcgggcc tggcgctggt cgacggcatc ctggcgcgcg gcgagctggc
       61 cgactaccgg ctggcgcacg cggcacgcgc ggacctgtgc cggcggctgg ggcgcatcga
      121 tgacgcacgc gactcctacc agcgcgcgct gtcactcacc cggcagcaag cagagcaacg
      181 cttcctgcag cgacggctcg atcagctgga gaaaaaatag tttccggcgg ctgtcgaaat
      241 ggccacaggc cgttcgacta ccccatgtca ggcgacacaa cgaaggagag tcccatgtcc
      301 agcaggatca cgccctttct ctggttcgac ggcaaggccg aggaggcggc aaagctctat
      361 tgctccatct tccccaactc gaagattacc caggtggtgc gcagcacggt ggatacgccg
      421 ggcacgccca ggggcggcgt gctcacggtg ggcttcacgc tcaacgggct ggagtacacc
      481 gccatgaacg gcgggcccat ggtcagcttc aacgagtcgg tgtcgttcgt cgtgcattgc
      541 aagacgcagg aagagatcga ccactactgg gacaggctca ccgccgacgg cggtgcgccg
      601 gtggcctgcg gctggctcaa ggacaagtac ggactgcgct ggcagatcac gcctgacctg
      661 ctgctcgagc tgatcgccag caaggacagc gcgcgcgccc agcgcgccat gcaggcgatg
      721 atgaagatgg tgaagatcga cctcaagaca ctggagaacg cgtgatggcc gtcgccaggg
      781 agattttcat caacctgccg gtcaaggacc tggacaagtc ggtcgcgttc ttccgcgccg
      841 tgggcctgtc gcttgatccc aggttcggcg acggcatgaa ctgcctggtc gtcggcgaca
      901 gcatccgcgc catgctgatg agccacgaac agttcgccgc cgtcatgccc ggtccgctgg
      961 ccgacgtgag caggccggct gcactgcggc tgtcgctgga aaatcgcgca gccatcgagg
     1021 cgctgatgga aaaagcgctg gccgccggcg gccgccgcta cgagccggcg caggaccacg
     1081 gcttcatgta cgaggatggc tttctcgacc tggacggtca ccactggagc ctgttccacc
     1141 tggactacgc cgcgttcgac cgcgcacgcc gggagcaggc cgcatgaaat atctctgcct
     1201 ggtgtacggc gaggaagaca agatcgccgc gatgaacgat ctcgaatgcc tggccttcga
     1261 ccgcgacgcg cgcgccagcg gccattgcgt ggcatcggag gcgctgcagc cggtgtccac
     1321 cgccagcacc gtgcgcgtgc gccagggacg gctgacggtg catgacggcc ctttcgccga
     1381 aaccaaggaa gcgctggccg gcttctacct gatcgaggcg cgcgacctca acgaggcgat
     1441 ccagctggcg gcgaagatcc cgccggcgca ggtcggctgc gtcgaggtgc gacccattcg
     1501 ccccatccgc gagcagccgc gcaacaccgc ggcgccgtcg cactcataag gagttccgtc
     1561 atgctcaagc agctcaatcc gtacatccat tttcatcgcg actgcgccga ggccatcgcc
     1621 ttctacgagc gcgtgctcgg cggcaaggtg gagatgatga tgaaggtcaa ggatgcgccg
     1681 ccgtcggcgc agatgaaacc ttcgaaggag aacgaaaacc tggtgctgca cgtgcgcctg
     1741 tcggtgggct cgtaccagat cctggcatcc gactggatct ccgaccagcc gttccagccc
     1801 atgcacggct tctcgctgtc gctggagtac cccacggtgg acgaagcacg caaggtgtac
     1861 gacgcgcttt ccgtcggcgc gcgcgaaatc cgcatgccgc tgcagggcac gttctgggcc
     1921 aaggccttcg ccatgttcgt ggaccagtac ggcacgccgt ggatgatcag cggcgcgatg
     1981 cagtgaagcg gggcattcag tccggaaaca cttcagtgct acgaaaaaag tagcaaccgc
     2041 accccggtct ggcctgttac taattattta ttgtttttac ttttgtaata ttttttataa
     2101 caggccggat ggcagtgcgg tgggaggttt tcggcgctct gcgcgccgcc tcac
//
LOCUS       DASZZC010000742         5088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_631254,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000742 DASZZC010000000
VERSION     DASZZC010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631254"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHE37_15850"
     CDS             <1..691
                     /locus_tag="VHE37_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HVT37063.1"
                     /translation="SRLSPHLHFGEISPLQVHARVQRLLAQEAGATAGAENFLREVGW
                     REFAHHLLFHFPHTTDAPLDERFAAMRWRKDHAEDLRRWQRGVTGIPIVDAGMRELWA
                     TGFMHNRVRMIVASLLTKNLLIPWQEGARWFWDTLVDADLANNTLGWQWVAGCGADAA
                     PYFRVFNPRLQSEKFDAAAYIRRWVPELAQQPDAQIHAPPPVTGYPSPMVDLKATRER
                     ALDAWQRIRSS"
     gene            857..1081
                     /locus_tag="VHE37_15855"
     CDS             857..1081
                     /locus_tag="VHE37_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HVT37064.1"
                     /translation="MKLKITTIGNSAGVVLPKELLAHLRVGKGDELYVAETPDGIKLM
                     PHDPTFAAQMEVAERVMRKRRTMLRKLADS"
     gene            1084..1554
                     /locus_tag="VHE37_15860"
     CDS             1084..1554
                     /locus_tag="VHE37_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HVT37065.1"
                     /translation="MIVWIDKPLVLALHDRQIAEHGGSPGLRDERLLESALARPQQLH
                     AYGDPQPDLADLAASLAFGIAKNHPFIDGNKRSAIVSCETFVELNDATLEAGDAELYQ
                     AILFLAESKFTEQEFAAWLRTHINVRRRGVQEPRARYGAARKRAPARKPRRTRA"
     gene            1583..2554
                     /locus_tag="VHE37_15865"
     CDS             1583..2554
                     /locus_tag="VHE37_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HVT37066.1"
                     /translation="MSQHEFQALGRKVLEIERDALSALLPRVGADFARACEIMLACSG
                     RVVVTGMGKSGHIGNKIAATLASTGTPAFYVHPGEASHGDLGMITKGDVVLALSNSGE
                     TSEVLTILPLIKRMGAMLISMTGKPTSTLAKTADVHLDVSVAQEACPHNLAPTASTTA
                     ALAMGDALAVALLEARGFTPEDFAMSHPGGSLGRKLLLKISDLMHSGERLPKVSSDVP
                     LSAALLEMTRKGLGITAVVDAGGKLLGVYTDGDLRRTLEKDIDVRSAKIADVMTRGGK
                     TAQANWLAAQAVELMERHKITALLVVDESNALVGAIHMQDLLRAGVV"
     gene            2554..3063
                     /locus_tag="VHE37_15870"
     CDS             2554..3063
                     /locus_tag="VHE37_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HVT37067.1"
                     /translation="MLTAAAVRARAKKIRCLFLDIDGVMTDCKLYLAADGSELKAVNV
                     KDGYGMKQLMKHGIEVAVISGRPSEAMKKRFAFLGIRHYYLDTEEKIPAYESIRAKLG
                     LTDAQCAHMGDDVPDLPLFQRVGLALAVADAHPRALKAAHWKSRHVGGDGAIREAADL
                     ILAAQGKLK"
     gene            3060..3638
                     /gene="lptC"
                     /locus_tag="VHE37_15875"
     CDS             3060..3638
                     /gene="lptC"
                     /locus_tag="VHE37_15875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HVT37068.1"
                     /translation="MKLQVSTGSLMTLVLALLTAAALMLTLQNYQRAARGAAPTPEST
                     ETPRYILRSAQWTRLDAQGQPDYVADADLIEYFDDHSARLTQPVVHAFGGGNSPWRAA
                     APTGSTLPHSRSLALTGGVEVLGHWPDGEDVTIHTEHLWIDQAQKVLRTDAEVTVDSA
                     SRQLQARGMSADASGKRVQLLGQVRGVYAPRS"
     gene            3622..4170
                     /gene="lptA"
                     /locus_tag="VHE37_15880"
     CDS             3622..4170
                     /gene="lptA"
                     /locus_tag="VHE37_15880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HVT37069.1"
                     /translation="MRRARELSALVLGGWIACAAAADDKTQRAVDALRPTGPVTVTAD
                     HAQFEQGGAMLYVGHVQLVSDTLTLSGDKLELHQFPGNQFEAQITGAPAHLQHAGETN
                     DKGETLPPITAQASTLHYDTRNGTVDINGQALMTRGKDEISGDNMHYNVPARRIEASG
                     GNNGQVRMVLQPPPEKKPADKK"
     gene            4167..4895
                     /gene="lptB"
                     /locus_tag="VHE37_15885"
     CDS             4167..4895
                     /gene="lptB"
                     /locus_tag="VHE37_15885"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HVT37070.1"
                     /translation="MTGILAAKNLSKSYKKRAVVRDVSLDVRAGEIVGLLGPNGAGKT
                     TSFYMMVGLVAADGGSIELDGQNITALPMHARARLGIGYLPQEASVFRKLSVAENIMA
                     ILEMRDMLDDDARNAELERLLTELHIAHIRDGLGLSLSGGERRRVEVARALAASPRFI
                     LLDEPFAGVDPIAVADIQHIVHHLRERGIGVLISDHNVRETLGICSRAYILAEGRVIA
                     EGEPSALLQNETVRKVYLGEQFRL"
BASE COUNT          929 a         1702 c         1685 g          772 t
ORIGIN      
        1 ctcgcgcctg tcgccgcacc tgcatttcgg cgagatcagc ccgctccagg tacacgcgcg
       61 cgtgcagcgc ctgctcgcac aggaagcggg cgcgactgcc ggggcagaaa atttcctgcg
      121 cgaagtcggc tggcgtgaat tcgcgcatca cctgctgttc cactttccgc acactaccga
      181 tgcgccgctg gatgagcgct tcgccgcgat gcgctggcgc aaggatcacg ccgaggacct
      241 gcggcgctgg cagcgcggcg tcaccggcat acccatcgtc gacgccggca tgcgcgagct
      301 gtgggccacc ggcttcatgc acaaccgcgt gcgcatgatc gtcgccagcc tgctgaccaa
      361 gaacctgctg atcccgtggc aggagggcgc acgctggttc tgggacacgc tggtcgacgc
      421 cgacctggcc aacaacacgc tgggttggca atgggtggcc ggctgcggcg ccgatgcggc
      481 gccctacttc cgcgtgttca acccgcggct gcagtcggag aaattcgatg cggccgccta
      541 catccgccgc tgggtgccgg agctggcgca acaacccgac gcgcagatcc acgcgccgcc
      601 gccggtcacc ggatacccgt caccgatggt tgacctgaag gccactcgcg agcgcgcgct
      661 cgatgcatgg caacgaatca ggtcgtcatg acggcttttt ttggcctgac cgcaccgcct
      721 ttttcgatgt tattaattat tgttcgaatg tattttcgga taataattaa taacatcaaa
      781 aagcggcatg cggtggggcc gttttcgctc caggatcggt atgtacgtac gttataatca
      841 tgcccggagg cgtcccatga aactcaagat caccaccatc ggaaattccg ccggcgtggt
      901 tctgcccaag gaactgctcg cccacctgcg cgtgggcaaa ggcgatgaac tgtacgtcgc
      961 cgaaacgccg gacggcatca agctgatgcc gcatgacccg acgtttgcgg cacagatgga
     1021 agtagccgag cgcgtgatgc gcaagcgccg caccatgctg cgtaaactgg ccgacagcta
     1081 gcgatgattg tctggatcga caagccgctg gtcttggccc tgcatgaccg gcagatcgcc
     1141 gagcacggcg gaagccccgg cttgcgtgat gagcggttgc tggagtcggc gcttgcacgt
     1201 ccacagcagt tgcatgctta cggcgaccct cagcccgatc tagcagacct cgctgccagc
     1261 ctcgcttttg gcatcgccaa gaaccatcca ttcattgacg gcaacaagcg cagcgcgatc
     1321 gtcagctgtg aaaccttcgt tgaactgaac gatgccacgc tcgaagctgg cgacgccgag
     1381 ctttatcagg caattctgtt tctggctgaa agcaaattca ctgagcagga gttcgccgcc
     1441 tggctgcgca cgcacatcaa cgtgcgcagg cgcggcgtgc aggagccgcg tgcacgctac
     1501 ggcgcagccc gcaagcgcgc gcccgcgcgc aaaccgcgcc gcacccgcgc atagccgggc
     1561 gagcggttaa gatgcgcggc ctatgtccca gcatgaattc caggctctgg gccgcaaggt
     1621 cctggagatc gagcgcgacg cgctgagcgc actgctgccg cgcgtcggcg cagactttgc
     1681 ccgcgcgtgc gagatcatgc tggcgtgcag cggccgcgtg gtggtcaccg gcatgggcaa
     1741 gagcggccat atcggcaaca agatcgccgc cacgctggcc tccaccggta cgccggcgtt
     1801 ctacgtgcat ccgggcgagg ccagccatgg cgacctgggc atgatcacca agggcgacgt
     1861 ggtgctggca ttgtcgaatt ccggcgagac ctccgaagtg ctgaccatcc tgccgctgat
     1921 caagcgcatg ggcgcgatgc tgatcagcat gaccggcaag ccgacttcaa cgctggccaa
     1981 gacggccgac gtgcacctgg acgtgtcggt ggcgcaggag gcctgcccgc acaacctcgc
     2041 gccgaccgcc agcaccaccg ccgcgctggc gatgggcgat gcgctggcag tggcgctgct
     2101 cgaagcgcgc ggcttcacgc cggaagattt cgccatgtcg caccccggcg gctcgctcgg
     2161 acgaaaactg ttgttgaaga tttctgacct gatgcacagc ggcgagcgcc tgcccaaggt
     2221 gagcagcgac gtgccgctgt cggccgcgct gctggaaatg acgcgcaagg gcctgggcat
     2281 cactgccgtt gtcgatgccg gcggcaaatt gctcggcgtg tacaccgacg gcgacctgcg
     2341 acgcacgctg gaaaaggaca tcgatgtgcg cagcgcgaag atcgccgacg tcatgacccg
     2401 cggcggcaag accgcgcagg cgaactggct ggcggcgcag gcggtcgagt tgatggagcg
     2461 ccacaagatc accgcgctgc tggtggtgga cgagagcaac gcgctggtcg gcgccatcca
     2521 catgcaggac ctgctgcgcg cgggagtggt gtgatgctca cggctgccgc ggtgcgcgcg
     2581 cgcgcgaaga agatccgctg cctgttcctc gacatcgacg gcgtgatgac cgactgcaag
     2641 ctttatctgg cggccgacgg cagcgagctg aaagcagtga acgtcaagga tggctacggc
     2701 atgaagcagc tgatgaaaca cggcatcgag gtcgccgtga tctccggccg gccgtcggag
     2761 gcgatgaaga agcgcttcgc cttcctcggc atcaggcatt actacctcga caccgaggaa
     2821 aaaatcccgg cctacgaaag catccgcgcc aagctcggcc tcaccgacgc gcaatgcgcg
     2881 cacatgggcg acgacgtgcc cgacctgccg ctgttccagc gcgtgggcct ggcgctggcg
     2941 gtggccgacg cgcatccgcg cgcgctcaag gccgcacact ggaaatcacg ccacgtcggc
     3001 ggcgacggcg ccatccgcga agcggccgac ctgatcctgg ccgcgcaggg caagctgaaa
     3061 tgaagctgca ggtctccacc ggctcgctga tgacgctggt gctggcgctg ctcaccgcgg
     3121 ccgcgctcat gctcacgctg cagaactacc agcgggccgc gcgcggcgcg gcgccgacgc
     3181 cggaaagcac ggagacgccg cgctacatcc tgcgcagcgc gcagtggacg cggctcgatg
     3241 cgcagggcca gcccgactac gtggcggacg ccgacctgat cgaatatttc gacgatcatt
     3301 ccgcgcgcct gacccagccg gtggtgcatg ccttcggcgg cggcaactcg ccgtggcgcg
     3361 ccgcggcgcc cacaggcagc acgctgccgc acagccgcag cctggcgctg accggcggcg
     3421 tcgaggtgct gggccactgg cccgacggcg aggatgtgac catccacacc gagcacctgt
     3481 ggatcgacca ggcgcagaag gtgctgcgca ccgatgccga ggtcaccgtc gacagtgcca
     3541 gccgccagct gcaggcgcgc ggcatgagtg ccgacgccag cggcaagcgc gtgcagctgc
     3601 tcggccaggt gcggggcgta tatgcgccgc gctcgtgaac tctcggcact ggtgctgggc
     3661 ggctggatcg cctgcgcggc cgccgccgat gacaagaccc agcgcgcggt cgatgcgctc
     3721 aggccgaccg gcccggtcac ggtgaccgcc gaccacgcgc agttcgagca gggcggcgcc
     3781 atgctctacg tcggccacgt gcagctggtg tcggacacgc tcacgctgtc cggcgacaag
     3841 ctcgagctgc accagtttcc cggcaaccag ttcgaggcgc agatcaccgg cgcgccggcg
     3901 cacctgcagc acgccggcga aaccaacgac aagggcgaga cgctgccgcc gatcaccgcg
     3961 caggccagca cgctgcatta cgacacgcgc aatggcacgg tggacatcaa cggccaggcg
     4021 ctgatgacgc gcggcaagga tgagatcagc ggcgacaaca tgcattacaa cgtgccggca
     4081 cggcgcatcg aggcgtcggg cggcaacaac ggccaggtgc gcatggtgct gcagccgccg
     4141 cccgagaaaa aacccgcgga caagaaatga ccggcatcct cgccgcgaaa aacctgtcga
     4201 agagctacaa gaagcgcgcg gtggtgcgcg acgtgtcgct cgacgtgcgc gccggcgaga
     4261 tcgtcggcct gctcggcccc aacggcgccg gcaagaccac ctcgttctac atgatggtgg
     4321 gcctggtggc ggccgacggc ggcagcatcg agctcgacgg ccagaacatc accgcgctgc
     4381 cgatgcacgc gcgcgcgcgc ctcggcatcg gctacctgcc gcaggaagcc agcgtgttcc
     4441 gcaagctgag cgtggccgag aacatcatgg ccatcctgga aatgcgcgac atgctcgatg
     4501 acgacgcgcg caatgccgag ctggagcgcc tgctcaccga gctgcacatc gcgcatatcc
     4561 gcgacggcct cggcctgtcg ctgtccggcg gcgagcgccg ccgcgtggaa gtggcgcgcg
     4621 cactggccgc cagcccgcgc ttcatcctgc tcgacgaacc tttcgccggc gtggacccga
     4681 tcgcggtggc cgacatccag cacatcgtgc atcacctgcg cgagcgcggc atcggcgtgc
     4741 tgatttccga ccacaacgtg cgcgagacgc tgggcatctg cagccgcgcc tacatcctgg
     4801 cggaaggccg cgtgatcgcc gagggcgagc cgtcggcgct gctgcagaac gagacggtgc
     4861 gcaaggttta cctgggcgaa cagttccgcc tgtagggcgc gcctggcgcg cgatcaaacg
     4921 ctgcatggcg gcaaggcacg tcgctgaaaa tacgtctagc aagcatcgtg ccgccgtgcg
     4981 tagaatgcgg ccatgtgtcg cgctcttttt gatgttcctg gattccgctt aacactcggc
     5041 ggaggccgcg catgaagcag acgctcggcc tcaggatggg ccagtcgc
//
LOCUS       DASZZC010000743         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_695491,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000743 DASZZC010000000
VERSION     DASZZC010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695491"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..>1879)
                     /locus_tag="VHE37_15890"
     CDS             complement(22..>1879)
                     /locus_tag="VHE37_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HVT37071.1"
                     /translation="NPACLEIMSHEMLTHLGLLAEVDSLAGELSTWAEHSPGWPQARH
                     CQALVRRLLARVDTLRVRLDSPLVVATLGGTGTGKSTLVNALVGAEVTSAGRERPTTR
                     QPTLVCRPGLRLAMLGIDPDSVTVIERDAPVLRDLVLLDCPDPDTTEDEALRGTNLAR
                     LRELLPHCDVLLVATTQQKYRSARVTEELAGAAAGARLVFVQTHADVDADVREDWQRA
                     LAGDYSTGEMFFVDSLTAIAETRQGVKPRGEFGRLVDLLTRELTVAATHRIRRANFLD
                     LVGESLAACQERVASSMAQIEQLEAGIQEQRTRLAARLTERLREELLASRRSWESRLV
                     GEVTSRWGFSPFSCLLRLYQGLGGLVAGAALVRVRSAAQLALWGALEGGRRWRARRNE
                     RRGQSATTRAAALGWEESDLRTGAIIIDGYAAEAGLDRRELEPAVLQREAAAVANSFI
                     GDASAQLQAVINRQTARHCGLFTRAIYEVLFSVMCVALLYRFAKNFFYDSWLAPEWGF
                     ATTAQPVLGTDFFLGAALVLGAWSALLLWSFTSRLRRGLSAEINEVAQAWASPKLTAG
                     FFADLSRQCRDIHAWHDELRRLSSQVADLQARLAGPELLGHRVGARGHGRAE"
BASE COUNT          330 a          678 c          592 g          279 t
ORIGIN      
        1 tcgcgaggac atctacagat gctattccgc gcgcccatga ccgcgcgcgc cgacgcgatg
       61 gcccagcagt tccggcccgg ccaagcgcgc ctgcagatcg gcaacctggc tgctcaagcg
      121 ccgtagctcg tcgtgccagg cgtgaatgtc gcggcattgc cgggacagat cagcgaaaaa
      181 cccggccgtg agcttggggc tggcccacgc ctgcgccact tcgttgattt ccgcgctcag
      241 tccgcgccgc agccgcgagg tgaacgacca cagcagaagc gcactccaag cccccagcac
      301 cagcgccgcg ccgagaaaga aatccgtccc cagcaccggc tgggccgtcg tggcgaatcc
      361 ccattccggc gcgagccacg agtcgtagaa aaaattcttg gcaaagcggt agagcagtgc
      421 cacgcacatc acggaaaaca gaacttcata gatggcgcgg gtgaacagcc cgcagtgccg
      481 cgcagtttgc cggttaatga cggcttgcag ttgagccgag gcgtcgccaa tgaagctgtt
      541 ggcgaccgcg gcggcctcgc gttggaggac cgctggttcg agttcgcggc gatcgagccc
      601 ggcctcggcc gcgtagccat cgatgatgat ggcgcccgtg cgcaagtcgg attcctccca
      661 accgagcgcc gcggcgcgcg tggtcgccga ctgaccgcgg cgctcattgc ggcgcgcccg
      721 ccagcggcgg cctccttcca gcgcgcccca cagagccagt tgtgccgcgc tgcgcacgcg
      781 caccagcgcc gcgcccgcca caaggccgcc cagcccctga tacaggcgca gcaggcagga
      841 gaacgggctg aacccccacc gcgacgttac ttcccccacc agccggctct cccagctgcg
      901 ccggctggcc agcaattctt cacgcagccg ctccgtcaac cgtgcggcga gccgggtgcg
      961 ctgttcctgg ataccagcct ccagttgctc gatctgcgcc atgctgctgg caacgcgttc
     1021 ctggcaggca gcgagcgatt caccgaccag gtccagaaaa ttcgcgcggc ggatgcgatg
     1081 cgtcgccgcc accgtcagct cgcgcgtgag caaatccacc agccggccga attcgccgcg
     1141 cggcttgacg ccttgtcgcg tctcggcgat ggccgtcagc gagtcgacga aaaacatctc
     1201 gccggtcgag taatccccgg caagtgcgcg ctgccaatct tcgcgcacgt cggcatcgac
     1261 gtcggcgtgt gtttgcacga aaaccaggcg cgcgccggcc gcggcgcccg ccaactcctc
     1321 cgtcacccgc gcgctgcgat acttctgctg tgtcgtcgcc accagcagca cgtcacaatg
     1381 cggcaaaagc tcgcgcagcc tggccaggtt ggtgccgcgc agtgcttcgt cttcggtcgt
     1441 gtcgggatcg gggcaatcca agagcacgag atcgcgcagc accggcgcgt cgcgctcgat
     1501 cacggtcacc gaatcggggt cgatccccag catcgccagc cggagtcccg gacggcaaac
     1561 gagcgtcggc tgccgggtcg tgggacgttc gcgtccggcg gaagtgactt cagccccgac
     1621 gagtgcattc accagcgtgc tttttccggt gccggtgccg ccgagcgtgg ccacgaccaa
     1681 gggcgaatcg agccgcacgc gcaacgtgtc aacgcgcgcc agcaaccgcc gcaccaacgc
     1741 ctggcaatga cgcgcttgcg gccagccagg actgtgctcg gcccacgtcg agagctcacc
     1801 cgccagcgag tcgacctcgg ccagcaagcc caggtgcgtc agcatttcat gcgacatgat
     1861 ttccagacac gccgggttc
//
LOCUS       DASZZC010000744        10152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_631566,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000744 DASZZC010000000
VERSION     DASZZC010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631566"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VHE37_15895"
     CDS             <1..1314
                     /locus_tag="VHE37_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37072.1"
                     /translation="APGRFAGSAGCGEVVDVMRTCASHILALRAGAALQPGARRYTRA
                     WIRDATVMAAALLRMGRVGEVQSFIDWYAGFLRADGYVPCCVDAQGPDWTVEHDSHGQ
                     WLSLLTDHAALAADDACLHRHWPSIERAAQLIEQLLDASGLLPPSISHEGYAAQPVHA
                     YWDDFWAIRGLQDAARAAQRLGRHPQAHRWTLLAGRIAAATSASIAATCARAGIDYWP
                     GSRELADFDPTATASAVVLLDGSALLDVAALQRTYDRYMNDWRRRRSGQLDWVSYTPY
                     EIRNIGALVRLGRRADAIELLEFFMAGRRPLRWNQWPEIAWRDVQAPAHVGDVPHGWV
                     AAEFVLAARSLFLYERDADRCLVIAAGLPPAWLTGEGVRVTDAPTAYGPVSYRLQHDG
                     RRLSFSGTFPAKVVRVELHSPLPVGTPVDISAAGTVHICYPRSAA"
     gene            1311..4526
                     /locus_tag="VHE37_15900"
     CDS             1311..4526
                     /locus_tag="VHE37_15900"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HVT37073.1"
                     /translation="MNDVIYQIGDGHYQAWIDEHGCGGAKLGDLALYRSGGDAPRDRT
                     GFFIYLRDADSGRLWSAARRPVRYTPQHHRVHRAGAMLQIESGFDDIDSMVLLEANST
                     GEARTVRLRNRSARRRTIELTSYLEVIAGDGQADRAHPAFAKLFVQTARSPDGTGLVA
                     VRRPRAGDEHGPALRHSLSGAPLLAWETDRCCFIGRGRNTARPLLRMRGTVGNVLDPM
                     LSLRASVELPPGGEATVTFRLEAAAHFATMPATNADVSTPDAAVTGAFCNSGDEYRIG
                     LPWQGDQPALPPMPWINVIANRGFGCMVSETGAGSTWSRNSQMHRLTPWFNDPVSDPH
                     GEALYLRDEGSGEFWSPLPGPCPAPVDHQVHHGFGYSRFAAVSHQLEQDTTVFVARED
                     AVKFSLIRLHNRSDQARTLSLFSHQQLVLGERPRRASGVRTWRSGSWLGAQPPPDSAY
                     AGPLTYGTAVADVAAEIFTGCDRDSFLGDGDLRAPQALLCPQLPGDQSGGEPCFTWQL
                     RFRLGAGQSAAIAILLGEALDAAELDRVREQYASVIEVSAELDAVRAAWTQRLSRLRV
                     RTPEPAIDRMVNGWLPYQAWSCRLWARSAYYQSSGAFGFRDQLQDAGNTCLLWPELAR
                     QQLLLHAGRQFAEGDVQHWWHEAPIDRGIRTRCSDDLLWLPYVACQYIERTGDRAVLD
                     ERAGFLLAPELAGNEADRYLQAERSTGTFSIYEHCCRAIERALTRGAHGLPLIGSCDW
                     NDGYNRLGRRGLGESVWLAFFLHRVLDDFGSLAQARGDHPRAQRYAAVRAQLREALER
                     HGWDGQWYRRAYDDDGVPLGTASSAECRIDGLAQSWAVLSGAAPDARAREALASADAQ
                     LVDEPHQLIRLLTPPFGEGAADPGYIKGYLAGVRENGGQYTHAACWLIEAMARAGRRD
                     RAAHLLTLISPLQHTRSAPAMARYQLEPYVIAADIYGAEPHAGRGGWSWYTGSAGLAW
                     RVAVEAVLGLRVEGGSVLSLRPCIPDEWPGYDIEYTEPSSSTRYCIEVVNADRCAQAI
                     VAAQLDGVPVAELGQEIRWPLQRDGARHRVIVQLGRKPGAA"
     gene            complement(4489..6312)
                     /locus_tag="VHE37_15905"
     CDS             complement(4489..6312)
                     /locus_tag="VHE37_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HVT37074.1"
                     /translation="MSQINRRHLRGLMHVNATGARAADAAQVPTRPHRPPAPAADQLI
                     GAQRFGRDSWRRLRGEAAALLRSGQGPAALRFALGLLTLLALLNGLNVINSYVGRDFM
                     SAIANRNARLFVHQAWLYVGVFALSTIAAVLCRNLEERLGLLWRNWQTRRLLQQYLAQ
                     RVYQRIEASHGLQNPDQRIAEDVRSFTTSTLSFALMMLNASITLVAFSGVLWSISPKL
                     FLVAVAYALGGSLVTIALGRRMVRLNSQQLDREANLRSELLHLRENAEAIALQQREAD
                     IYARLRQRLDQLVANTLRLISIHRNVGFFTSGYNYLIQIVPALIVAPLYFRGQVEFGV
                     VTQSAMAFSQLMGAFSLLVTQFESISSYAAVIGRLGHLVDAMEDAPAATGAAAQQDHD
                     LAIEYRGLTLHGPQGETLLNALRLRIDRGTRLLVTSDSGHAKIALLRATAGLATSDEG
                     TVITPPAKDLMFVPERPYLPHGSLREILSAQASSDAELQRVLRDLGLEALDSGPAALD
                     LEADWSSRLGIREQALLMMARVLLAKPAFVFIDRMSVALDPPHIARTLQTFTDAGISY
                     VVLGREAPVAAQFNARLHIAADGSWTLNTLRPRPASVPAAR"
     gene            6365..8743
                     /locus_tag="VHE37_15910"
     CDS             6365..8743
                     /locus_tag="VHE37_15910"
                     /inference="COORDINATES: protein motif:HMM:NF003617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HVT37075.1"
                     /translation="MDDVKQPAPPARASSQQEIAAIDRWWRAANYLSAGQIYLAANPL
                     LREPLQLAHIKPRLLGHWGTAPGINFIYAHLNRVIRARHARMLLVTGPGHGGPALLAN
                     GWLEGSYSDIYPAVGQDLAGMRQLFRQFSFPGGVPSHASPETPGSMHEGGELGYALSH
                     AYGAAFDNPDLVVACIVGDGEAETGPLATSWHSNKFVDPVSDGAVLPILHLNGYKIAG
                     PTVLARIPHAELEQLLRGYGHDPLFVEGEQPHDMHGQMMRAMDLALDRIAEIQQQARS
                     QARRGRPAWPMIVLRSPKGWTGPREVDGKPVENSFRSHQVPVAELARADHLEVLDRWL
                     RSYRPEELFDAQGCPRPDLRALAPEPALRLSLSPQANGGSLLRPLRLPDIARYAVSVV
                     QPGAGCAESTAVQGRLVRDVVRQNPDNFRVFSPDETASNRWSAVFEATDRVFAGQIVA
                     SDEHLAARGRVMEMLSEHQCQGWLEGYLLSGRHGFFSCYEAFIHIIDSMLNQHAKWLK
                     VAREVPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFLDHVVNKKAEFIRIYLPPDAN
                     TLLCVTDHCLRSRNLINVIVAGKQPEPQWLTLEQARAHCSAGIGTWDWAGNEQGSTPD
                     VVMACCGDVPTLETLAAVDLLRRHAPELRVRVINVVDLMKLQPASEHPHGLSDDEYVA
                     LFTARQPVVFAFHGYPWLIHRLAYRRPNHEHLHVRGYKEEGTTTTPFDMTVRNDLDRY
                     HLAMAALQRVPRMAARRAELSARFTAQLDAHRAYIREHGEDMPEIRDWKWSR"
     gene            8740..9849
                     /locus_tag="VHE37_15915"
     CDS             8740..9849
                     /locus_tag="VHE37_15915"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HVT37076.1"
                     /translation="MKRQLVVNAGSATLKWTLFGAGRAKLDGGVVSWNSAEPEHCGAQ
                     MRELLRGIGEFEAAGHRLVHGGARYTAPVALTGEVRAQLQALCALDPDHMRPALACID
                     TVAGACPQVTQFASFDTAFHATLPEAAARYALPREWIDRWQLRRYGFHGLSVDYSVRR
                     VRELLGMVPARMVVCHLGSGCSLTAVRDGRSVDTTMGYTPLDGPVMATRSGAIDPGLL
                     LQVQRQAGLDADAMQDALTHRSGLLALSGISGDLGQVLAAAGRGDQRAASAYDCFLHS
                     LRRYLGAMIAVLQGVDAVVFTGGMAEHSPRLRQDLCETLRFAGLEIDPGKNREGASDR
                     LISAPRASIAAYLIHAREDRVVLDDLLRLTSKDTA"
     gene            9846..>10152
                     /locus_tag="VHE37_15920"
     CDS             9846..>10152
                     /locus_tag="VHE37_15920"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HVT37077.1"
                     /translation="MNAPTAELRALGQSLWLDSISRQLLDAGTLARYIGEFGVSGVTS
                     NPAIFHKAIACGGDYDEELQRLWQQGRRGEDLVFDLAIGDIRGAADLLAPVHRASAGA
                     "
BASE COUNT         1554 a         3476 c         3577 g         1545 t
ORIGIN      
        1 gcgccgggcc ggttcgcggg cagtgcgggc tgcggcgaag tcgtcgacgt catgcgcacc
       61 tgcgcctcgc acatcctcgc gctgcgtgcc ggcgccgcgc tgcagccggg cgcgcggcgt
      121 tacacgcgtg cgtggatacg cgatgcgacg gtcatggcgg ccgcgttgct gcgcatgggt
      181 cgcgtgggcg aagtgcaatc cttcatcgac tggtacgccg gctttctgcg ggccgatggc
      241 tacgtgccct gctgcgtcga tgcccagggc cccgactgga cggtcgaaca cgacagccat
      301 gggcaatggc tctcgctgtt gaccgatcac gcggcgctgg cggcagacga cgcctgcttg
      361 caccggcact ggccctcgat cgagcgggct gcgcagctga tcgaacagct gctcgacgcc
      421 agcggcctgc tgccgccttc catcagccat gaaggctatg cggcgcagcc ggtgcacgcg
      481 tactgggatg acttctgggc catccgcggc ttgcaggacg cggcacgcgc cgcgcagcgc
      541 ctgggccggc atccgcaggc gcatcggtgg acgctcctgg ccggacggat cgccgccgcc
      601 acatcggctt cgatcgcggc gacctgcgcg cgcgcgggca tcgattactg gccgggcagc
      661 cgcgaactgg cggatttcga tcccaccgcc accgccagcg cggttgtgct gctcgacggt
      721 tctgcgctgc tcgatgtcgc cgcgctgcag cgcacctacg atcgctacat gaatgactgg
      781 cgccgcaggc gcagcggcca actggactgg gtcagctaca cgccctacga gatccgcaac
      841 atcggcgcgc tggtgcgcct gggccgccgc gccgacgcca tcgaactgct ggaattcttc
      901 atggccgggc ggcgcccgct gcgctggaac cagtggccgg aaatcgcctg gcgcgacgtg
      961 caggcgcccg cgcatgtggg cgacgtgccg catggctggg tggccgcaga gttcgtgctg
     1021 gccgcgcgca gcctgttcct gtacgagcgc gatgccgacc gctgcctggt gatcgcggcc
     1081 ggtcttcctc cagcctggct gacgggtgaa ggcgtgcgcg tgacggacgc gccgacggca
     1141 tatggccccg tcagttatcg cctgcagcat gacgggcgcc ggctcagctt ttccggaaca
     1201 tttccggcga aggtggtgcg ggtggaattg cactcgccgt tgccggtggg gacgccggtc
     1261 gacatcagcg ccgcgggcac cgtgcatatc tgctatccgc ggagcgccgc gtgaacgacg
     1321 tgatctacca aattggcgat ggccactacc aggcctggat cgacgaacac ggctgtggtg
     1381 gcgcgaaact aggcgacctc gccttgtacc ggtccggcgg cgatgcaccg cgtgaccgta
     1441 ccggcttctt catctacctg cgcgatgccg acagcggccg gctgtggagc gcggcgcgcc
     1501 ggccggtgcg ctacacgccg cagcaccatc gcgtgcatcg cgcgggcgcc atgctgcaga
     1561 tcgagtcggg cttcgacgac atcgacagca tggtgttgct cgaagcgaac tcgacgggtg
     1621 aggcacgcac cgtgcgcctg cgcaaccggt ccgcgcgccg gcgcacgatc gagctcacca
     1681 gctacctgga agtgattgcc ggggacgggc aggcggatcg cgcgcacccg gcatttgcca
     1741 agctgttcgt gcagaccgca cgcagccccg atggaaccgg cctggtggcc gtgcgccggc
     1801 cgcgcgcggg cgacgaacat gggccggcac tgcggcacag cctgagcgga gcgccgctgc
     1861 tggcctggga aaccgaccgc tgctgtttca tcggccgcgg ccgcaacacg gcgcgcccgc
     1921 tgctgcgcat gcgcggcacg gtgggcaacg tgctcgaccc catgctcagc ctgcgtgcct
     1981 cggtcgagtt gccgcccggc ggcgaagcga cggtgacatt ccgtctcgaa gcggcggcgc
     2041 atttcgctac catgcctgcg acgaacgcgg acgtatcgac gcccgacgcg gcggtcaccg
     2101 gtgcgttctg caacagcggc gatgaatatc gaatcggctt gccctggcag ggcgaccagc
     2161 cggcgctgcc gccgatgccg tggatcaatg tcatcgccaa ccggggtttc ggctgcatgg
     2221 tcagcgagac cggcgcgggt tccacctgga gccgcaacag ccagatgcac aggctgacgc
     2281 cgtggttcaa cgacccggtg agcgatccgc acggcgaggc gctgtacctg cgcgacgagg
     2341 gcagcggcga attctggtcg ccgctgccgg ggccttgccc ggcgccggtg gatcaccagg
     2401 tgcatcacgg gttcggctac agccggttcg ccgcggtgag ccaccagctt gaacaggaca
     2461 ccaccgtgtt cgtggcgcgc gaggatgccg tgaagttttc gctcatccgc ttgcacaacc
     2521 ggtcggacca ggcgcgtacg ctttcacttt tttcccatca gcaactggtg ctgggcgaac
     2581 ggccgcgccg cgccagcggc gtcagaacct ggcgcagcgg ctcctggctc ggtgcccagc
     2641 cgccgccgga cagcgcatac gcagggccgc tgacctacgg caccgcggtg gccgacgtcg
     2701 ctgcggaaat attcaccgga tgcgaccgcg acagcttcct cggcgatggc gatctgcgtg
     2761 cgccgcaggc cttgctctgc ccgcagcttc ccggagatca aagcggcggc gagccgtgct
     2821 tcacctggca gctgaggttc aggctcggcg ccgggcaaag cgccgccatc gcgatcctgc
     2881 tcggcgaggc gctcgacgcc gctgaactcg atcgcgtgcg cgagcagtac gccagcgtga
     2941 tcgaggtcag tgccgaactg gatgccgtgc gcgcggcgtg gacgcagcgc ctgtcgcggc
     3001 tgcgcgtgcg cacgccagaa ccggccatcg accgcatggt caacggctgg ctgccctacc
     3061 aggcctggtc ctgccgactg tgggcgcgca gtgcgtatta ccagtcgagc ggggcattcg
     3121 gttttcgcga ccagctgcag gatgccggca acacctgcct gctgtggccg gagctggcgc
     3181 ggcaacaact gctgttgcac gcggggcgcc agttcgccga gggcgacgtg cagcactggt
     3241 ggcacgaggc gccgatcgac cgcggcatcc gcacgcgctg ctcggacgac ctgctgtggc
     3301 tgccgtacgt ggcctgccag tacatcgagc gcaccggcga ccgggccgtg ctcgacgagc
     3361 gcgcaggttt tctgctggcg ccggaactgg ccggcaacga agccgaccgt tacctgcagg
     3421 cggaacgcag cacaggaacc ttcagcatct acgagcactg ctgccgtgcc atcgagcgcg
     3481 cgctgacgcg cggcgcgcac ggcctgccgc tgatcggcag ctgcgactgg aatgacggct
     3541 acaaccgcct gggccgccgc ggcctcggcg aaagcgtgtg gctggccttt ttcctgcacc
     3601 gggtgctgga cgatttcggc agcctggcgc aggcccgcgg cgaccatccg cgcgcgcagc
     3661 gctatgccgc ggtccgcgcg cagctgcgcg aggcgctgga gcggcacggc tgggacgggc
     3721 agtggtaccg ccgcgcctac gacgatgacg gcgtgccgct gggcacggca tcgtcagccg
     3781 aatgccgcat cgacggcctg gcgcagtcct gggcagtgct gtcgggtgct gcgccagacg
     3841 cgcgtgcccg cgaggcactt gccagtgccg atgcgcagct ggtcgacgag ccgcatcaac
     3901 tgatccgcct gctgacgccg ccatttggcg agggcgccgc tgatccgggc tacatcaagg
     3961 gttacctcgc cggcgtgcgc gagaacggcg ggcagtacac acacgcggcg tgctggctga
     4021 tcgaggcgat ggcgcgcgcc ggccggcgcg accgggccgc gcatctgctc acgctgatca
     4081 gcccgctgca gcacacgcgc tccgcgccgg cgatggcgcg ctaccagctc gagccctacg
     4141 tgatcgcggc ggacatctac ggcgcggagc cgcacgcggg gcgcggcggc tggagctggt
     4201 acaccggctc tgccggcctg gcctggcgcg tcgcggtgga agcagttctt ggcctgcgcg
     4261 tggaaggcgg cagcgtactg tcgctgcggc cgtgcatccc ggacgagtgg ccgggttacg
     4321 acatcgagta caccgagccc tcttcgtcga cgcgctactg catcgaagtg gtgaacgccg
     4381 accgctgcgc gcaggcgatc gtcgccgcgc agctcgacgg tgttccggtc gccgagctgg
     4441 ggcaggagat ccgctggccg ctgcagcgcg acggcgcaag gcaccgcgtc atcgtgcagc
     4501 tgggacggaa gccgggcgcg gcctgagcgt gttcaaggtc cagctgccat ccgccgcgat
     4561 gtgcaggcgg gcgttgaact gcgcggcaac gggcgcttcg cggccaagca cgacgtagct
     4621 gatgccggca tcggtgaagg tctgcagcgt gcgcgcgatg tgcggagggt cgagcgccac
     4681 gctcatgcgg tcgatgaaaa cgaaggccgg ttttgccagc agcacgcgcg ccatcatcag
     4741 cagcgcctgc tcgcggatgc ccagccggct gctccagtcg gcttcgagat ccagcgcggc
     4801 ggggccgctg tccagcgcct ccagtcccag gtcccgcagc acgcgctgca actcggcatc
     4861 ggacgatgcc tgcgcggaaa gaatctcgcg caggctgccg tgcggcaggt atggccgctc
     4921 cggcacgaac atcaagtcct tggcgggcgg cgtgatgacg gtgccctcgt cgctggtggc
     4981 gaggccagcg gtggcgcgca gcagcgcgat cttggcgtgg ccggaatcgc tggtcaccag
     5041 caggcgcgtg ccgcggtcga tgcgcaggcg cagcgcgttc agcagcgtct cgccctgcgg
     5101 gccgtgcagc gtgagcccgc gatactcgat ggcaaggtca tggtcctgct gcgctgcagc
     5161 gccggtggca gccggcgcgt cctccatcgc atcgactaga tgtcccagcc ggccgatcac
     5221 cgccgcgtaa ctggagatcg actcgaattg cgtcaccagc agcgagaacg cgcccatcag
     5281 ctgcgagaag gccatggccg attgcgtgac cacgccgaac tccacctggc cgcggaagta
     5341 cagcggcgcc acgatcagcg cgggcacgat ctggatcagg tagttgtagc cgctggtgaa
     5401 gaagccgacg ttgcggtgga tggaaatcag ccgcagcgtg ttggcgacca gctggtccag
     5461 ccgctgccgg agccgggcat agatatccgc ttcgcgttgc tgcagggcga tggcctcggc
     5521 gttctcgcgc aggtgcagca gctccgagcg caggttggct tcgcggtcca gctgctggct
     5581 gttgagccgg accatgcgcc ggcccagcgc gatggtcacc agcgatccgc cgagcgcata
     5641 ggccaccgcc accaggaaga gtttcgggct gatcgaccac agcaccccgg agaaggccac
     5701 cagggtgatc gaggcattca gcatcatcag cgcgaacgac agcgtgctgg tggtgaagct
     5761 gcgcacgtct tcggcgatgc gctggtcggg gttctgcagg ccgtggctgg cctcgatgcg
     5821 ctggtacacg cgttgcgcca ggtattgctg cagcagccgg cgcgtctgcc agttgcgcca
     5881 cagcaggccg agccgttcct ccaggttgcg gcacagcact gcagcgatgg tcgacagcgc
     5941 gaacacgccg acgtagagcc aggcctggtg cacgaacagc cgcgcgttgc ggttggcgat
     6001 ggccgacatg aaatcgcggc cgacgtaact gttgatcacg ttaagcccgt tgagcagcgc
     6061 cagcagcgtc agcaggccga gtgcgaagcg cagcgcggcg ggcccttggc cggacctgag
     6121 cagcgcggcc gcctcgccgc gcaggcggcg ccagctgtcg cggccaaagc gctgcgcgcc
     6181 gatcaattga tcggcggccg gcgcaggtgg tcggtgaggg cgggtgggca cttgcgcagc
     6241 atctgccgcg cgcgcgccgg tggcattgac gtgcatcaag ccgcgcaggt gccggcgatt
     6301 gatctggctc aatgagatgc cgctccgcgg gcggtagctt gcacgctgcc caccggatcg
     6361 tgaaatggat gacgtgaaac agccagcgcc accggcgcgc gccagcagcc agcaggagat
     6421 cgcggccatc gaccgctggt ggcgcgcggc caattacctg tcggcgggcc agatctacct
     6481 ggcggccaat ccgctgctgc gtgagccgct gcagctggca cacatcaagc cccgcctgct
     6541 cggccactgg ggcaccgcgc cgggcatcaa cttcatctac gcgcatctca accgcgtgat
     6601 ccgcgcgcgc catgcccgca tgctgctggt caccggcccg ggccacggcg ggccggcgct
     6661 gctggccaac ggctggctcg aaggcagcta cagcgacatt tatcccgcgg tgggccagga
     6721 ccttgccggc atgcggcaac tgttccggca gttctccttt cccggcggcg tgcccagcca
     6781 tgcatcgccg gaaacgccgg gctcgatgca cgaaggcggc gaactcggtt acgcgctgtc
     6841 gcacgcgtac ggcgctgcgt tcgacaaccc ggacctggtc gttgcctgca tcgtcggcga
     6901 tggcgaagcc gagaccgggc cgctggccac cagctggcac tcgaacaagt tcgtcgatcc
     6961 ggtgagcgat ggcgcggtcc tgcccatcct ccatctcaac ggctacaaga tcgccggacc
     7021 caccgtgctg gcgcgcatcc cgcacgcgga actggagcaa ttgctgcgcg gctacgggca
     7081 cgatccgctg ttcgtcgaag gcgagcagcc gcatgacatg catggccaga tgatgcgcgc
     7141 catggacctg gcgctggacc gcatcgccga gatccagcag caggcgcgca gccaggcaag
     7201 gcgcgggcgc cctgcctggc cgatgatcgt gttgcgcagc cccaagggct ggaccggtcc
     7261 gcgcgaggtt gacggcaagc cggtggaaaa cagtttccgt tcgcaccagg tgccggtggc
     7321 ggaactggcg cgcgcggatc acctggaagt gctcgaccgc tggctgcgca gttaccggcc
     7381 ggaagaactg ttcgatgcgc agggatgtcc gcggccagac ctgcgcgcac tggcgccgga
     7441 gcccgcgttg cgcctgagcc tgagcccgca ggccaatggc ggatcattgc tgcggccgct
     7501 gcggctgcca gacattgcgc gttacgccgt gagcgtggtg cagcccggtg ccggctgcgc
     7561 ggaatccacc gcggtgcagg gacgcctggt gcgcgatgtc gtgcggcaga accccgacaa
     7621 cttccgcgtg ttcagcccgg acgaaaccgc gtcgaaccgc tggagcgcgg tgtttgaagc
     7681 gaccgaccgc gtgttcgccg ggcagatcgt tgcatccgac gagcacctgg ccgcgcgcgg
     7741 gcgcgtgatg gaaatgctca gcgagcacca gtgccagggc tggctggaag gctacctgct
     7801 gtccggtcgg catggatttt tttcctgcta cgaagccttc atccacatca tcgactcgat
     7861 gctcaaccag catgccaagt ggctgaaggt ggcgcgcgag gtgccgtggc gccggccgat
     7921 cgcatcgctc aactacctgc tcagctcgca cgtgtggcgg caggaccaca acggcttctc
     7981 gcaccaggac ccgggctttc tcgaccacgt ggtcaacaag aaggcggagt tcatccgcat
     8041 ctacctgccg ccggatgcca acacgctgct gtgcgtcacc gaccactgcc tgcgcagccg
     8101 caacctgatc aacgtcatcg tcgccggcaa gcagccggag ccgcagtggc tcacgctgga
     8161 gcaggcgcgc gcgcactgca gcgccggcat cggcacctgg gattgggccg gcaacgagca
     8221 gggcagcacg cccgacgtgg tcatggcctg ctgcggcgat gtgccgacac tggagacgct
     8281 ggccgcggtc gacctgttgc ggcgccacgc gccggagctg cgcgtgcgtg tgatcaacgt
     8341 ggtggacctg atgaagctgc agccggccag cgagcatccg catggactca gcgatgacga
     8401 gtacgtcgcg ctgttcaccg cgcggcagcc ggtggtgttc gcctttcacg gttacccgtg
     8461 gctgatccac cggctggcct accgccgccc caaccatgag cacctgcacg tgcgcgggta
     8521 caaggaggaa ggcaccacca ccacgccgtt cgacatgacc gtgcgcaatg acctggaccg
     8581 ctatcacctg gccatggccg cgctgcagcg cgtgccgcgc atggccgctc ggcgcgcgga
     8641 actcagcgcg cgcttcaccg cgcagctgga cgcgcaccgc gcctacatcc gcgagcacgg
     8701 cgaagacatg cccgagatcc gcgactggaa atggagccgg tgaagcgcca gctggtggtg
     8761 aacgcgggct cggccacgct caagtggacc ctgttcggcg ccggccgcgc gaaactggac
     8821 ggcggcgtgg tcagctggaa cagcgccgag ccggagcatt gcggcgcaca gatgcgcgaa
     8881 ctgctgcgcg gcattggcga gtttgaagct gcgggccatc gactggtgca cggcggcgcg
     8941 cgctacaccg cgccggtggc gctgaccggc gaggtgcgcg cgcagctgca ggcgctgtgc
     9001 gcactcgatc ccgaccacat gcggccggcg ctcgcctgca tcgacacggt tgccggcgcc
     9061 tgtccgcagg tgacgcagtt cgcgtcgttc gataccgcgt tccacgccac gctgcccgag
     9121 gccgccgcgc gctacgcgct gccgcgcgag tggatcgacc gctggcagtt gcgccgctac
     9181 ggcttccacg ggctcagcgt cgattactcg gtgcggcgcg tacgcgagct gctgggcatg
     9241 gtgccggcgc gcatggtcgt ctgccatctc ggcagcggct gctcgctgac cgcggtgcgc
     9301 gacgggcgct cggtcgacac caccatgggc tacacgccgc tcgacggccc ggtgatggcc
     9361 acgcgttccg gcgccatcga tccgggcctg ctgctgcagg tgcagcggca ggcaggactc
     9421 gacgccgatg ccatgcagga tgcgctgacc catcgctccg gcctgctcgc gctgtcggga
     9481 atttccggcg acctgggcca ggtgctcgct gccgcgggcc gcggcgatca acgtgccgcc
     9541 tcggcctatg actgctttct gcacagcctg cgccgctacc tcggcgcaat gatcgcggtg
     9601 ctgcagggcg tggatgcggt ggtgttcacc ggcggcatgg cggagcacag cccgcgcctg
     9661 cggcaggatc tgtgcgagac gctgcgcttc gccggcctgg agatcgaccc gggaaaaaat
     9721 cgcgaaggcg cgagcgatcg cctgatttcc gcgccgcgcg cttccattgc cgcatacctc
     9781 atccacgccc gcgaagaccg cgtggtgctc gacgacctgc tgcggctgac ttcaaaggac
     9841 accgcgtgaa cgcgcccacc gccgaactgc gcgcgctcgg gcagagcctg tggctcgaca
     9901 gcatcagccg ccagctgctc gatgccggca ccctggctcg ctacatcggc gagttcggcg
     9961 tgagcggcgt gacctcgaac ccggccatct tccacaaggc catcgcctgc ggcggcgact
    10021 acgacgagga gttgcagcgc ctgtggcagc agggccggcg cggcgaagac ctggtgttcg
    10081 atctcgccat cggtgacatc cgcggcgcgg cggacctgct ggcgccggtg catcgcgcca
    10141 gcgctggcgc gg
//
LOCUS       DASZZC010000745         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_882337,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000745 DASZZC010000000
VERSION     DASZZC010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882337"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1692
                     /gene="acpA"
                     /locus_tag="VHE37_15925"
     CDS             31..1692
                     /gene="acpA"
                     /locus_tag="VHE37_15925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HVT37078.1"
                     /translation="MKLRMRTLLAGAAPAAAIGLMTGLSACSTDNTVGGAPASGVKNI
                     VVIYAENRSFDALFGNFPGANGVPTDPAKYTQLDRDGVTPLAKLPQTWGGVTAAGNPL
                     VVTQAQSDNLPNAPFAIETTFNAVNGASVLSTDDVTRDLTHRFFENIMEINGGSNDMF
                     AAWLDAGGLTMGHFDYSKSALYKLAQQYVLADNFFEGAYGGSFLNHQYLICACAPSVP
                     AGAAGVVGTVVGNGMSVNVLGTPNAKNVPQLARTDANVSAIDGPAAYKTGNIAPLDYF
                     GAGDGYRAVNTMQAPYQPSGNFPAAGAADLRYANPAAANTLPPQTAKTIGDQLTAKNL
                     SWAWYAGAWNAATVDGEQPSNATHSIIYTPSTPRAAPDFQAHHHPFNYYAAFDPATHG
                     ADRVTHLKDGNDLLDDAANGTLPAVAFYKPQGSVNQHPGYANAADGDAHIADVVAKLQ
                     ASPQWKNMVIVITYDEYGGQWDHVAPPKGDLLGPGTRIPAIIVSPFAKMGTVDHTLYD
                     TGSVQRLINKVFGTAPLPGITARDAALVAGGGSAMGDLTNALNLP"
     gene            complement(1886..>2387)
                     /locus_tag="VHE37_15930"
     CDS             complement(1886..>2387)
                     /locus_tag="VHE37_15930"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT37079.1"
                     /translation="FTRITQDAWDEQLVTNLRTAFAASKAAIPHLRAGGGIVNIASIT
                     GQMPVPTTPAYGAAKAGMISLTRSLALELAPKQIRVNAICPGLLWTRAWEMLAQMIQA
                     TSPALKELSPREIFLDRVMKLVPMGAEQTAEDIGALAVFLCSDGARQITGQAIAVDGG
                     ATLKVG"
BASE COUNT          430 a          853 c          730 g          374 t
ORIGIN      
        1 ctacaccaac cagtcctggg agacagcagc atgaaactgc gtatgcggac cctgcttgcc
       61 ggcgccgcgc cggcggcggc gatcggattg atgaccggcc tgagcgcctg cagcaccgac
      121 aacaccgtgg gtggcgcgcc ggcctccggc gtcaagaata tcgtggtgat ctacgccgag
      181 aaccgcagct tcgacgcgct gttcggcaac ttccccggcg ccaatggcgt gccgaccgac
      241 ccggccaagt acacgcagct cgaccgtgat ggcgtgacgc cgctggccaa gctgccgcag
      301 acctggggcg gcgtcaccgc cgccggcaat cccctcgtgg tcacgcaggc gcagtcggac
      361 aacctgccca acgcgccgtt cgccatcgaa accactttca atgcggtcaa cggcgcctcg
      421 gtgctgtcga ccgacgacgt cacccgcgat ctcacccacc ggttcttcga gaacatcatg
      481 gagatcaacg gcggcagcaa cgacatgttt gccgcctggc tcgatgccgg cggcctgacc
      541 atgggccact tcgactacag caagtccgcg ctgtacaagc tggcgcagca gtacgtgctg
      601 gccgacaact tcttcgaagg cgcctacggc ggctcgttcc tgaatcacca gtacctgatc
      661 tgcgcctgcg cgcccagtgt gcccgcgggt gcggccggcg tcgtcggcac cgtggtcggc
      721 aatggcatgt cggtcaacgt gctgggcacg cccaacgcga agaacgtgcc gcagctggcg
      781 cgcaccgacg ccaacgtgtc ggccatcgac ggcccggccg cgtacaagac cggcaacatc
      841 gcgccgctgg attacttcgg cgccggtgac ggctaccgcg cggtcaacac catgcaggcg
      901 ccgtatcagc ccagcggcaa cttccccgcg gccggcgcag ccgacctgcg ctacgccaac
      961 cccgctgcgg ccaacacgct gccgccgcag accgccaaga ccatcggcga ccagctgacc
     1021 gcgaagaacc tgagctgggc ctggtacgcc ggcgcgtgga acgcggcgac ggtcgacggc
     1081 gagcagccgt ccaacgccac gcacagcatc atctacacgc cgtcgacgcc gcgcgccgcg
     1141 ccggacttcc aggcgcatca ccatccgttc aactactacg cggcattcga cccggcgaca
     1201 cacggcgccg atcgcgtcac ccacctgaag gacggcaacg atctgctgga tgacgccgcc
     1261 aatggcacgc tgcccgcggt cgcgttctac aagccgcagg gcagcgtcaa ccagcacccg
     1321 ggctatgcca acgcggcgga tggcgacgcg cacatcgccg acgtcgtggc caagctgcag
     1381 gccagcccgc agtggaagaa catggtcatc gtcatcacct acgacgagta cggcggccag
     1441 tgggaccacg tggcgccgcc caagggcgac ctgctcggcc ccggcacgcg catccctgcg
     1501 atcatcgttt cgcccttcgc caagatgggc acggtggatc acacgttgta cgacaccggc
     1561 tcggtgcagc gcctgatcaa caaggtgttc ggcacggcgc cgctgcccgg catcaccgcg
     1621 cgcgacgccg cgctggtggc cggcggcggc agcgcgatgg gtgatctcac caacgcgctc
     1681 aacctgccgt agaaaatttt ttcctgcggt acacgacacg ggcagcgcga gctgcccgta
     1741 gtcgtttgcg cggccgaaaa acacctccac cgcactgccc tttatgatgt cattaattat
     1801 ttaatttatt ttacataata caattatata taacatcagg aatagcggtg cggtggaaga
     1861 ctttttggcc ctcatccacc tactttcagc ctactttcag cgtcgcaccg ccatccaccg
     1921 cgatggcctg cccggtgatc tgccgagcgc cgtcgctgca caggaacacc gccagcgcgc
     1981 cgatgtcttc cgcggtctgc tccgcgccca tcggcaccag cttcatcacc cggtcgagaa
     2041 agatctcgcg cggtgaaagc tccttcagcg ccggcgacgt tgcctggatc atctgcgcca
     2101 gcatctccca ggcgcgcgtc cacagcaggc ccgggcagat ggcattgacg cggatctgtt
     2161 tcggcgccag ttccagcgcc agcgagcgcg tcagcgagat catgccggcc ttggccgcgc
     2221 cgtaggccgg cgtggtcggc accggcatct gcccggtgat cgaggcgatg ttgacgatgc
     2281 ccccgcccgc gcgcaggtgc gggatggccg ccttgctcgc agcgaaggcg gtgcgcaggt
     2341 tggtgacgag ttgctcgtcc cacgcatcct gcgtgatgcg cgtgaac
//
LOCUS       DASZZC010000746         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_118405,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000746 DASZZC010000000
VERSION     DASZZC010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118405"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..336)
                     /locus_tag="VHE37_15935"
     CDS             complement(28..336)
                     /locus_tag="VHE37_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37080.1"
                     /translation="MRIVRLAALAGLLLLAGCADEPRAPYGTPALRKQLLEQFKAADK
                     DGNDNLTRDEAAAGMPSVAAHFDEIDTDHNERVNFAEIWNYAQWRGIADRRRSDDRAP
                     "
     gene            361..1134
                     /gene="ubiE"
                     /locus_tag="VHE37_15940"
     CDS             361..1134
                     /gene="ubiE"
                     /locus_tag="VHE37_15940"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HVT37081.1"
                     /translation="MADQKMSSTRKPGTTHFGYQQVPVEEKQKRVAGVFTSVAGKYDL
                     MNDLMSLGVHRLWKRFVIDLACVRPGEKVLDVAGGTGDLTREFARQVGERGLVVLSDI
                     NAAMLGEGRARLTDKGVIGVPLAQANAEKLPFAEGAFDLITIGFGLRNVTDKDAALAS
                     MRRCLKPGGRLLVLEFSKPLSEPLSKVYDQYSFKLLPMLGRLVANDADSYRYLAESIR
                     MHPDQQTLKGMMERAGLERVQVYNLTGGIVAVHRGFRLE"
     gene            1131..>1633
                     /locus_tag="VHE37_15945"
     CDS             1131..>1633
                     /locus_tag="VHE37_15945"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HVT37082.1"
                     /translation="MKAPDLLLAAVEVALNRFLGLEADALAECAKLAGRAIALHANGP
                     EWTFFIEFHPGGVRVLPTMPRTPNVSISATPLVLLRQGLHAGSGGALPQGLTVEGDVA
                     LLTRFQELLARVGFDPEELAAKYLGDAAAHRLVQGAEKLFGWGRKTAQTLALDTSEYL
                     REETRDLA"
BASE COUNT          266 a          546 c          540 g          281 t
ORIGIN      
        1 agcgctacgc atcaaccatg aactgcttca cggggcgcga tcatcgctcc gccgtctgtc
       61 ggctattccg cgccactgcg cgtaattcca gatttcggcg aagttcaccc gctcgttgtg
      121 gtcggtatcg atctcgtcga aatgcgccgc cacgcttggc atgccggcgg cggcttcgtc
      181 gcgcgtgagg ttgtcgttgc catccttgtc cgcggccttg aactgctcca gcagctgctt
      241 gcgcagcgcc ggcgtgccgt aaggcgcgcg cggctcgtcg gcgcaaccgg ccagcagcag
      301 gagtcccgcc agggcagcaa gccttacaat gcgcatcgtt ttacatcccg ctgatcaaat
      361 ctggctgatc aaaaaatgtc cagcacccgc aagcccggca ccacgcactt cggttaccag
      421 caagtgccgg tggaagagaa gcagaaacgc gtggccggtg tgttcacgtc ggtggcgggc
      481 aagtatgact tgatgaacga cctgatgtcg ctgggcgtgc atcgcctgtg gaagcgcttc
      541 gtcatcgacc tggcctgcgt gcgccccggc gaaaaagtgc tcgacgtggc cggcggcacc
      601 ggcgacctca cgcgcgagtt cgcgcgccag gtgggcgagc gcggcctggt ggtgctgtcc
      661 gacatcaacg ccgccatgct cggcgaaggc cgcgcgcggc tcaccgacaa gggcgtgatc
      721 ggcgtgccgc tggcgcaggc caatgcggag aagctgccgt ttgccgaggg cgccttcgac
      781 ctgatcacca tcggtttcgg cctgcgcaac gtcaccgaca aggacgccgc gctggcctcc
      841 atgcgccgct gcctcaagcc cggcggccgg ctgctggtgc tggaattttc caagccgctg
      901 tcggagccgc tgtcgaaagt gtatgaccag tactcgttca agctgctgcc gatgctgggc
      961 aggctggtgg ccaacgacgc ggactcgtac cgctatctgg ccgaatcgat ccgcatgcac
     1021 ccggaccagc agacgctcaa gggcatgatg gaacgtgccg gcctggagcg cgtgcaggtg
     1081 tacaacctca ccggcggaat tgttgccgta caccgcggct tccgcctgga atgaaagcgc
     1141 cggacctgct gctggccgcg gtcgaggttg ccctcaaccg ctttctcggc ctggaagccg
     1201 acgcccttgc ggagtgcgca aagcttgcgg gccgagccat cgcgctgcac gcgaatggcc
     1261 ccgagtggac cttcttcatc gagttccatc ccggcggcgt gcgcgtgctg ccgaccatgc
     1321 cgcgcacgcc caacgtttcc atcagcgcca cgccgctggt gctgctgcgc cagggcctgc
     1381 acgcgggctc cggtggcgcg ctgccgcagg gcctcaccgt ggaaggtgac gttgcgttgc
     1441 tcacgcgctt ccaggaattg ctggcgcgcg tcggcttcga ccccgaagag ctggcggcga
     1501 aatacctggg cgacgcggcc gcgcaccggc tggtgcaggg cgccgaaaaa ttgttcggct
     1561 ggggccgcaa gacggcgcag acgctggcgc tcgacacctc ggaatacctg cgcgaggaaa
     1621 cgcgcgacct cgc
//
LOCUS       DASZZC010000747         5367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_60367,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000747 DASZZC010000000
VERSION     DASZZC010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60367"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VHE37_15950"
     CDS             <1..804
                     /locus_tag="VHE37_15950"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HVT37083.1"
                     /translation="RAALMFVLSKIVGTLLAPATVLFALLLLSWLLHARRPRASRRLL
                     GLALALLALLGLAPVSNWVLHPLESRFAPPSGLHHVDGIVVLGGAIDAGGSAYAHDVE
                     LNAHAERLTTLVALARRYPQARMIYSGGSADVLQKRQLREADLAPSFFEGLGLSPDRV
                     ELERDSRNTWENALYSQRLAQPRPGETWLLVTSAWHMPRAVGCFRELHWAVVPYPVDY
                     QSRSTGWGGFDIATKLEMLTEGSKEWIGLVSYRLLGRIPELLPGPETGA"
     gene            801..2282
                     /gene="astD"
                     /locus_tag="VHE37_15955"
     CDS             801..2282
                     /gene="astD"
                     /locus_tag="VHE37_15955"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HVT37084.1"
                     /translation="MSTQGGLYIGGQWRSGGGPLLSSVNPSDGEVIWNGAAASLKDVN
                     DAAVAARRASADWAALEFPAREKIVRRFAALLERDREKLARAIGAETGKPLWDARGEV
                     TAMINKIDISVRAYHARTGTSEAQAGAVKNIVRHKPHGVVAVFGPYNFPGHLPNGHIV
                     PALLAGNCVLFKPSELTPHVAEETVQRWHEAGLPHGVLALLQGERATGEAIAAHPQLD
                     GLYFTGSARTGHILAQQFAGQPGKILALEMGGNNPLVVGHLGDDLARRGALHDVIQSA
                     YLSSGQRCTCARRLYVPRGNAGDAFIAALADAVRAIKVGAYDADPQPYMGPLVSSRAA
                     QQLLAAQQQLLDLGAHALVPMKALEAGPAYVSPALIEVSQVAELPDEEHFGPLLQLAR
                     YDTLEQAIELANATRYGLAAGLLSDQPAQWQQFYRDIRAGIVNWNRQTTGASSSAPFG
                     GIGASGNHRASAWYAADYCAYPVATMELPRPELPAQLSPGLSL"
     gene            2352..2978
                     /locus_tag="VHE37_15960"
     CDS             2352..2978
                     /locus_tag="VHE37_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HVT37085.1"
                     /translation="MRTLSTPLIVCEHCDAVYRRRELPDGATARCLRCDASIDHRGRR
                     DLSTLVALALASLVTFCIANAYPIVAIEMEGSGDHSQTTLWGAVVATWRSGVGPLAVL
                     AWACAFFLPLAEILANLYVAVPLSLGRVPRGFNAAMHTLRLLRPWSMMEVFMLGSFVA
                     VVKLGSYAAVVVGIGLYAFAALTLLLTLLGSYELRDLWAQAQHARAAA"
     gene            2975..3592
                     /locus_tag="VHE37_15965"
     CDS             2975..3592
                     /locus_tag="VHE37_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HVT37086.1"
                     /translation="MSQVITARELGVVGCHACALVCRAPPAHSDAACPRCGARLHARK
                     PDSLTRSWAMLITAAILYIPANLLPMMDTTNILEQRSDTIVSGVIQLWDIGSWDLAII
                     VFTASVLVPLMKILVLGLLLYSVQRRSHWDPLERARLYRFIEFIGRWSMLDIFVVALL
                     ISLVNFDRFGEVRAGPGSIAFGAVVVLTMLSSLSFDPRLIWDEHT"
     gene            3589..5223
                     /locus_tag="VHE37_15970"
     CDS             3589..5223
                     /locus_tag="VHE37_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HVT37087.1"
                     /translation="MSDEQLPPELPEPVLQPRRRLAPSLIWLVPAIAALAGLILVVRT
                     IVSAGPSITITFKTAEGIEEGKTEVRYKNVVMGKVRSIKLTPDRQHVDVQVQLVKAAS
                     DLAVEDTRFWIVRPRVDLGGVSGLNTLLSGSYIGMDAGVSEESRRDFEGLEVPPAITH
                     DQKGRRFLLHAEDLGSLNLGSPVYFRHLPVGRIVGFNLNADGKAVTLQAFIDDPYDQY
                     VTQNVRFYNASGVSAQVGANGVQINMQSLLSLVAGGVVFELPPAMDGGGAVSGDTLFQ
                     LYDDRNMAMAPPDGPPLQIAMRFFQSVRGLAPNAPIDFRGLELGKVKTVVVDYDRGEH
                     AFAGLVTAEIYPQRMGAAFRKYHKMEVGDSGRAEATFHDLIDKRSLRAQLRSSNLLTG
                     ALYVALDFMPNVKSLPPNPDARVLEIPTVAGSLDQIQTQIADIVKKLDDVPFGDIGNN
                     LRDTLASANGLLKELDHDLAPEARRTLEEVRKTVDTLNQNLSTPDAPLAGDLHQTLQD
                     VDRAAHAMKNLADYLQRHPESLVRGKPKGDEPEESK"
BASE COUNT          817 a         1886 c         1832 g          832 t
ORIGIN      
        1 cgcgctgctc tgatgttcgt gctgtcgaaa atcgtcggca cgctgctggc tccggccacc
       61 gtgctgttcg cactgctgct gttgtcgtgg ctgctgcacg cgcgccggcc gcgggccagc
      121 cggcgcctgc tcggcctggc gctcgcactg ctggcgctgc tcgggctggc gccggtgtcg
      181 aactgggtgc tgcacccgct ggagtcgcgc tttgcgccgc cgtccggcct gcatcacgtg
      241 gacggcatcg tcgtgctggg cggcgccatc gacgccggcg gcagcgccta tgcgcacgac
      301 gtcgaactca atgcccacgc cgagcggctg accacgctgg tggcgctggc gcggcgctat
      361 ccgcaggcgc ggatgatcta cagcggcggc agtgccgacg tgctgcagaa gcggcagctg
      421 cgcgaagcgg acctcgctcc ttcgttcttc gaaggcctgg gactttctcc cgaccgggtg
      481 gaacttgagc gcgattcgcg caacacctgg gagaacgcgc tgtacagcca gcgcctggca
      541 cagccgcgcc ccggcgaaac ctggctgctg gtgacctccg cctggcacat gccgcgcgcg
      601 gtcggctgct tccgcgaact gcactgggcg gtggtgccct acccggtcga ctaccagagc
      661 cgcagcaccg gctggggcgg gttcgacatc gccaccaagc tggagatgct gaccgagggc
      721 agcaaggaat ggatcggcct ggtcagctat cgtctgctcg ggcgcattcc cgaactgctg
      781 cccggaccgg agactggcgc atgagcacgc aaggcggttt gtacatcggt gggcaatgga
      841 ggagcggcgg cggcccgctg ctgtcgagcg tcaatccatc cgacggcgag gtgatctgga
      901 acggcgccgc cgcctcgctc aaggatgtga acgatgcagc ggtggcggcg cgccgcgcca
      961 gcgcggactg ggccgcattg gagtttcccg cgcgcgaaaa gatcgtgcgc cgctttgccg
     1021 cgctgctcga acgtgaccgg gaaaagctgg cgcgcgccat cggcgcggag accggcaagc
     1081 cgctgtggga tgcgcgcggc gaagtgacgg cgatgatcaa caagatcgac atctcggtgc
     1141 gtgcctacca cgcgcgcacc ggcaccagcg aggcgcaggc gggcgcggtg aagaacatcg
     1201 tgcggcacaa gccgcacggc gtggtcgcgg tgttcggccc gtacaacttc cccggccacc
     1261 tgcccaacgg ccacatcgtg ccggcattgc tggccggcaa ctgcgtgctg ttcaagccta
     1321 gcgaactgac gccgcacgtg gccgaggaaa ccgtgcagcg ctggcacgag gccggcctgc
     1381 cgcacggcgt gctggcgctg ctgcagggcg agcgcgccac cggcgaggcc atcgccgcgc
     1441 atccgcaact cgacggcctg tatttcaccg gcagcgcgcg caccgggcat atcctcgcgc
     1501 agcagttcgc cgggcagcct ggaaaaatcc tcgcactgga aatgggcggc aacaatccgc
     1561 tggtcgtggg ccacctgggc gatgacctcg cgcgccgcgg cgcgttgcac gacgtgatcc
     1621 agtccgccta cctgtccagc ggccagcgct gcacctgcgc gcgccgcctg tacgtgccgc
     1681 gcggcaacgc gggcgacgcc ttcatcgccg cgctggccga cgccgtgcgc gcgatcaagg
     1741 tcggcgccta cgatgccgat ccgcagccct acatggggcc gctggtgtca tcgcgcgccg
     1801 cgcagcagct gctggccgcg caacagcagc tgctcgacct gggcgcgcac gcactcgtgc
     1861 cgatgaaggc gctcgaggcc gggccggcct acgtctcgcc ggcgctgatc gaagtgagcc
     1921 aggtggccga actgcccgac gaggaacact tcggcccgct gctgcagctg gcgcgctacg
     1981 acacgctgga gcaggccatc gagctggcca acgccacgcg ttatggactg gccgcaggcc
     2041 tgctgtcgga ccagccggcg cagtggcagc agttctaccg cgacatccgc gccggcatcg
     2101 ttaactggaa ccggcagacc acgggcgcct cgagttcggc gccgttcggc ggcatcggcg
     2161 ccagcggcaa ccaccgcgcc agcgcctggt acgccgccga ttattgcgcc tacccggtcg
     2221 ccaccatgga actgccgcgg ccggaattgc cggcccagct ctcgcccggg ctgtcgctct
     2281 gacagcgccg atcaaaccgc gctaggcttg ccgctgtctg atcggtagcg ccgagaatcc
     2341 caaggacaac catgcgcacg ctgagcaccc cgctgatcgt ctgcgagcac tgcgacgcgg
     2401 tgtaccgccg ccgcgagctg cccgacggcg ccaccgcgcg ctgcctgcgc tgcgatgcat
     2461 cgatcgatca tcgcggccgc cgcgacctct ccacgctggt ggcgctggcg ctggcatcgc
     2521 tggtcacctt ctgcatcgcc aatgcctacc cgatcgtcgc catcgagatg gaaggctcgg
     2581 gcgatcacag ccagaccacg ctgtggggcg cggtggtggc cacctggcgt tcgggcgtgg
     2641 gcccgctggc ggtgctggcc tgggcctgcg ccttcttcct gccgctggcg gaaatcctgg
     2701 ccaatctcta cgtcgccgtg ccgctgtcgc tgggccgcgt gccgcgcggc ttcaatgcgg
     2761 ccatgcacac gctgcgcctg ctgcggccgt ggagcatgat ggaagtgttc atgctcggca
     2821 gtttcgtcgc cgtggtgaaa ctgggcagct acgcggcggt ggtggtcggc atcggcctgt
     2881 atgccttcgc cgcactcacc ctgctgctga ccttgctcgg cagctacgaa ctgcgcgacc
     2941 tgtgggccca ggcgcagcac gcgcgggcgg ccgcatgagc caggtgatca ccgcccgcga
     3001 actgggcgtg gtcggctgcc acgcctgcgc gctggtgtgc cgcgcgccgc cggcgcacag
     3061 cgacgccgcc tgcccgcgct gcggcgcgcg cctgcatgcg cgcaaaccgg actcgctgac
     3121 ccgcagctgg gccatgctga tcaccgccgc catcctgtac atcccggcca acctgctgcc
     3181 gatgatggac accaccaaca tcctcgagca gcgctccgac accatcgtct ccggcgtgat
     3241 ccagctgtgg gacatcggct cgtgggacct ggccatcatc gtgttcaccg ccagcgtgct
     3301 ggtgccgctg atgaagatcc tggtgctggg cctgctgctg tactcggtgc agcggcgcag
     3361 ccactgggac ccgctggaac gcgcgcgcct gtaccgcttc atcgagttca tcggccgctg
     3421 gtcgatgctc gacatcttcg tcgtcgcgct gctgatctcg ctggtcaact tcgaccgctt
     3481 cggcgaggtg cgcgcaggtc ccggctcgat cgccttcggt gcggtggtgg tgctgaccat
     3541 gctgtcgtcg ctgtcgttcg atccgcggct gatctgggac gagcacacat gagcgacgaa
     3601 caactgccgc ccgaactgcc cgagccggtg ctgcagccgc gacgccggct ggcgccctcg
     3661 ctgatctggc tggtgccggc catcgccgcg ctggccggtc tgatcctggt ggtgcgcacc
     3721 atcgtcagcg cgggtccctc gatcaccatc accttcaaga ccgcggaagg catcgaggaa
     3781 ggcaagaccg aggtgcgcta caagaacgtg gtgatgggca aggtgcgcag catcaagctg
     3841 acgccggacc gccagcacgt ggacgtgcag gtgcagctgg tcaaggccgc ctccgacctg
     3901 gcggtggagg acacgcgttt ctggatcgtg cgcccgcgcg tggacctggg cggcgtgtcc
     3961 ggcctcaaca cgctcctgtc gggcagctac atcggcatgg acgccggcgt gtcggaggaa
     4021 tcgcgccgcg acttcgaggg actggaagtg ccgccggcca tcacccacga ccagaagggc
     4081 cgccgcttcc tgctgcatgc cgaggacctg ggctcgctca atctcggctc gccggtgtac
     4141 ttccgccacc tgccggtggg ccgcatcgtc ggcttcaacc tcaacgccga cggcaaggcg
     4201 gtgacgctgc aggccttcat cgacgatccg tacgaccagt acgtgacgca gaacgtgcgt
     4261 ttctacaacg ccagcggggt cagcgcgcag gtcggcgcca acggcgtgca gatcaacatg
     4321 cagtcgctgc tgtcgctggt ggcgggcggc gtggtgttcg aactgccgcc ggccatggac
     4381 ggcggcggcg cggtcagcgg cgacacgctg ttccagctct acgacgaccg caacatggcg
     4441 atggcgccgc cggacggccc gccgctgcag atcgccatgc gcttcttcca gtcggtgcgc
     4501 gggctggcgc cgaacgcgcc gatcgatttc cgcgggctgg agctgggcaa ggtcaagacc
     4561 gtggtggtcg actatgaccg cggcgagcat gctttcgccg ggctggtcac ggcggaaatc
     4621 tacccgcagc gcatgggcgc cgcgttccgc aagtatcaca agatggaagt gggcgacagc
     4681 gggcgcgccg aggccacctt ccacgacctg atcgacaagc gctcattgcg tgcccagctg
     4741 cgcagcagca acctgctcac cggcgcgctg tacgtggcgc tggatttcat gcccaacgtg
     4801 aaatcgctgc cgccgaaccc cgatgcgcgc gtgctggaga ttcccaccgt cgccggcagc
     4861 ctcgaccaga tccagacgca gatcgccgac atcgtcaaga agctcgacga cgtgccgttc
     4921 ggcgacatcg gcaacaacct gcgcgacacg ctggccagcg ccaacggtct gctcaaggag
     4981 ctcgatcacg acctggcccc ggaagcgcgc cgcacgctgg aagaagtgcg caagacggtg
     5041 gacacgctca accagaacct gtcgacgcct gacgctccgc tggccggcga cctgcaccag
     5101 acgctgcagg acgtggaccg cgccgcgcac gcgatgaaga acctggccga ttacctgcag
     5161 cgccatcccg aatcgctggt gcgcggcaag cccaagggcg acgagccgga ggagagcaaa
     5221 tgaaggcgaa gttcccgtgc gccgcgctcg gcgccgctgc agtgctgctc gcggcctgct
     5281 cgtcggcgcc ggtgcatttc tacacgcttg tcggcgcgcc aatcgcgccg gggctcgtag
     5341 gcgcgccggc cgcgcaggcg cccgccg
//
LOCUS       DASZZC010000748         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_511863,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000748 DASZZC010000000
VERSION     DASZZC010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511863"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1701)
                     /locus_tag="VHE37_15975"
     CDS             complement(145..1701)
                     /locus_tag="VHE37_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVT37088.1"
                     /translation="MATDFPSRAFADMSTLLAGQAAQLGNHPALVLDNRTLNYAELNQ
                     AADRVAAALQRDGIRPRDAVAFCARMSIEYVTALLGALRAGATVVPLQTTLRPQTLAQ
                     MMSDCSARLLFLDELNGVRLRKLERIDALRVALDNSDAGEPWQRWLAAPGTVPQPVVI
                     TPRSRFAIIYSSGTTGTPKGVVQSHGMRWAGMAAAPALGFDTRTVTLVSTPLYSNNTL
                     VTLLPTLAHGGRVVLMPTFDPRGFLELSVQHQVTHAMLVPTQYQRILALPEFDRYDLS
                     SYRWKFATGEHFPPALKAEVVRRWPGELAEFYGLTEGGGNTLLLASHYPDKLHTVGQP
                     VPGHSIRIVDDKGREVPVGEPGEIVGRAPGVMDGYLNRQRESIDLEWRDAAGYVYLRS
                     GDMGKFDAQGFLTLLERKKDLIISGGFKIYPSDLEGVLIVHEEVADVAVVGVPSAEWG
                     ETPVAFVVLRTAGRISADSLRAWADARLGKVQRLADLVLLPELPRSPVGKVLRRKLRD
                     GWLAQQRRRG"
     gene            complement(1714..4848)
                     /locus_tag="VHE37_15980"
     CDS             complement(1714..4848)
                     /locus_tag="VHE37_15980"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HVT37089.1"
                     /translation="MKIRHGALRPWSVVSACAGLLLAGSLQAQPPADVIRIDTGALSG
                     ARSGDVIAYKNIPYAAPPVGDLRWRAPQPPAAWDGVRDASAFGNDCMQNRMSWDKANS
                     AQPMSEDCLYLNVWAPATAAAKLPVMVWIHGGGFASGAGSSPLTLGDRLAARGAVVVT
                     FNYRLGRFGFFAHPALTAESGDGATGNYAFMDQIAALKWVQRNIAAFGGDPANVTIFG
                     ESAGGDSVHQLLLSDAARGLFAKAISQSGGGRALWPKLHDDQPGLPSAESTGVAFAAD
                     AGLKNADAAALRALPAKKVLGGISLLEHEKKTWSGPMIDGRIVSGNVVEGFAAGRGAK
                     VPLMIGANGDELGAIPFFLRGGINKEVLPRLGADADKVVAAYGSRDAFDHGIASDLNF
                     VEPARSIAAAVAATQPVYLYRFNYVTQAKRGDLKGAPHASDVPYVFDHLEASGEKIAD
                     ADRAEAQLIGQVWTSFARSGAPQVPGADWPPYSAKADQLLEFGNAGVSVASAGAAQLD
                     AISAHFAPAASATAAAPAPATISVAAPAPAAVAPKTACDLLADYQLDHGVIDSTASVA
                     PPDTLDIGLQGLPPLPVAAAYCRVRATLTPNPGSEIHIEVWLPPKEKWNGKYLGAGNA
                     GYAGDFASPWLYMGGAVAKGYATGGTDTGHRGHGMGKGEEAGASWALNAPDKIKDYGN
                     RAEHYTAIAAKLIVKAYYETPAKYAYFEGCSNGGREALMEAQKYPDDYDGIVAGAPAN
                     WWNNDQAGFAWNTLAARAAAIPAAKLQLLLDAVLKQCDALDGVADGLLEDPRMCKFDP
                     GKLQCKSGDADDCLSKVQVEAVRKIYGGPVDPRTHKSIFPGFPPGAEAVQWDEWITGA
                     SANQAMFSTEFFRYMVFGKPDWELSSLDFGKDIDLARSRTAADLDSANPDLRPFARHG
                     GKLIMYHGWDDAALTPLGSIRYYEDVKKKIGAKAQDDTMRLYLVPGMAHCIAGPGPNN
                     FDMLSQLERWVEQQQAPTQVIAAKYASDFAQLIGLPLGDPLRTRPLCPYPLKARWNGS
                     GSIDQAENFTCSAK"
BASE COUNT          788 a         1761 c         1692 g          818 t
ORIGIN      
        1 atggtcacgt gtgctcctcc cggagcgggt gcgccgctcc ccggcgacgc agcgctccgg
       61 cgccacgtct catattatgg gatttaattc tattatatgg gaccaaaaaa agcggcgcga
      121 gccgccgggc gccgctgccg ccgttcagcc ccggcggcgc tgctgcgcca gccagccgtc
      181 gcgcagcttg cggcgcaaca ccttgcctac cgggctgcgc ggcagttccg gcagcagcac
      241 caggtcggcc agccgctgca ccttgcccag ccgcgcgtcg gcccatgccc gcaggctgtc
      301 cgcggaaatg cggccggcag tgcgcagcac gacgaaggcc accggcgtct cgccccactc
      361 cgccgacggc accccgacca ctgccacgtc ggccacttcc tcgtgcacga tcagcacgcc
      421 ctccagatcg ctcgggtaga tcttgaagcc gcccgagatg atcaggtctt tcttgcgctc
      481 gagcagcgtg agaaagccct gcgcatcgaa cttgcccatg tcgccgctgc gcaggtagac
      541 gtagcccgcc gcatcgcgcc attccaggtc gatcgactcg cgctggcggt tgaggtagcc
      601 atccattacg ccgggcgcac ggccgacgat ttcgccgggc tcgcccaccg gcacttcgcg
      661 gcccttgtcg tcgacgatgc ggatgctgtg ccccggcacc ggctggccca ccgtgtgcag
      721 cttgtccggg taatggctgg ccagtagcag cgtgttgccg ccgccctcgg tcagtccgta
      781 gaactcggcc agttcgcccg gccagcgccg caccacctcg gctttcagcg ccggcggaaa
      841 gtgttcgccg gtggcgaatt tccagcgata gctggacagg tcgtagcggt cgaattccgg
      901 caacgccagg atgcgctggt actgcgtggg caccagcatg gcgtgcgtca cctggtgctg
      961 caccgacagt tcgagaaatc cgcgcggatc gaacgtgggc atcagcacca cgcggccgcc
     1021 atgggccagc gtcggcagca gtgtgaccag cgtgttgttg gagtacagcg gcgtcgatac
     1081 cagcgtcacc gtgcgggtat cgaagcccag cgccggcgcc gccgccatgc cggcccagcg
     1141 catgccgtgc gactgcacca cgcccttggg cgtgccggtg gtgcccgacg aatagatgat
     1201 ggcgaagcgc gagcgcggcg tgatcaccac cggctgcggc accgtgccgg gcgccgccag
     1261 ccagcgctgc cagggttcgc ccgcatcgga gttgtccagc gccacgcgca gcgcatcgat
     1321 gcgctccagc ttgcgcagcc gcacgccgtt gagttcgtcc aggaacagca ggcgcgcgct
     1381 gcagtcggac atcatctgcg ccagcgtctg cggccgcagc gtggtctgca gcgggaccac
     1441 cgtggcaccg gcgcgcagcg caccgagcag cgccgtcacg tattcgatcg acatgcgcgc
     1501 gcagaaggcc actgcgtcgc gcggacggat gccgtcgcgc tgcagcgccg ccgccacgcg
     1561 atccgccgcc tggttgagtt ccgcgtagtt cagcgtgcgg ttgtcgagca ccagcgccgg
     1621 atgatttccc agttgtgccg cctgcccggc aagcagcgtc gacatgtccg cgaacgcgcg
     1681 gctcgggaaa tccgtggcca tgcgcaagag tactcatttt gcgctgcagg tgaaattctc
     1741 ggcctggtcg atggaaccgc tgccgttcca gcgcgccttc agcgggtatg ggcacagcgg
     1801 ccgcgtgcgc agcgggtcgc ccagcggcag gccgatcagc tgcgcaaagt cgctggcgta
     1861 cttggccgcg atgacctgcg tgggcgcctg ctgctgctcc acccagcgct ccagctggct
     1921 gagcatgtcg aagttgttcg gcccgggccc ggcgatgcag tgcgccatgc ccggcaccag
     1981 gtacagccgc atggtgtcgt cctgcgcctt cgcgccgatc ttcttcttca cgtcctcgta
     2041 gtaacggatc gagcccagcg gcgtgagcgc cgcgtcgtcc cagccgtggt acatgatcag
     2101 cttgccgccg tgccgggcga acgggcgcag gtcgggattg gcggaatcga ggtcggccgc
     2161 ggtccggctg cgcgccaggt cgatgtcctt gccgaaatcc agcgatgaca gttcccagtc
     2221 cggcttgccg aacaccatgt agcggaagaa ctcggtggag aacatggcct ggttagccga
     2281 cgcgccggtg atccactcgt cccactgcac cgcctcggcg ccgggcggga agccggggaa
     2341 gatgctcttg tgcgtgcgcg gatcgaccgg accgccgtag atcttgcgca ccgcctcgac
     2401 ctgcaccttg ctcaggcagt cgtcggcgtc gccggacttg cactgcagct tgcccggatc
     2461 gaacttgcac atgcgcggat cttccagcag gccatcggcc acaccgtcga gcgcgtcgca
     2521 ctgcttgagc accgcgtcca gcagcagctg cagcttggcc gcaggaatcg cggcggcgcg
     2581 cgccgccagc gtgttccagg caaaaccggc ctggtcgttg ttccaccagt tggccggcgc
     2641 accggcgacg atgccgtcat agtcgtcggg atatttctgc gcctccatca gcgcctcgcg
     2701 tccgccgttg gagcagcctt caaagtacgc gtacttcgcc ggcgtctcgt agtacgcctt
     2761 gacgatcagc ttggcggcga tggcggtgta gtgctcggcg cggttgccgt agtccttgat
     2821 cttgtccggt gcattcagcg cccagctggc gccggcctcc tcgcccttgc ccatgccgtg
     2881 accccggtgc ccggtgtcgg tgccgccggt ggcatagccc ttggccaccg cgccgcccat
     2941 gtacagccag ggcgaggcaa agtcgccggc gtagcccgca ttgcccgcgc ccaggtactt
     3001 gccgttccat ttctccttcg gcggcagcca cacctcgatg tggatctccg agcccgggtt
     3061 aggcgtgagc gtggcgcgca cgcgacaata tgcagcagcc accggcagcg gcggcaaacc
     3121 ctgcaggccg atatcgaggg tatccggcgg cgccacgctg gcggtgctgt cgatgacccc
     3181 gtgatcgagc tggtagtccg ccagcaggtc gcaggccgtt ttcggtgcaa cggcggccgg
     3241 cgccggcgct gctacggaaa tcgtagcagg agcaggagca gcggccgtgg cggatgccgc
     3301 cggcgcgaag tgcgcgctga tcgcatcgag ctgcgctgcg ccggcgctgg cgacgctgac
     3361 gccggcgttg ccgaactcca gcagctggtc ggccttggcg ctgtaaggcg gccagtccgc
     3421 gccaggcacc tgcggcgcgc cggacctggc gaacgaggtc catacctggc cgatcagctg
     3481 cgcctcggcg cggtcggcgt cggcgatctt ctcgccgctg gcctccaggt gatcgaacac
     3541 gtagggcacg tcggaggcat gcggcgctcc cttgagatcg ccacgcttgg cctgcgtcac
     3601 gtagttgaag cggtacaggt agaccggctg cgtagcggcc acagccgccg cgatgctgcg
     3661 cgcgggctcg acgaaattca ggtcggaggc gatgccatga tcgaaggcat cgcgcgagcc
     3721 gtaggcggca accaccttgt cggcatccgc gcccaggcgc ggcagcactt ccttgttgat
     3781 gccgccgcgc aggaagaatg gaatggcgcc cagttcgtcg ccgttggcgc cgatcatcag
     3841 cggcaccttc gcaccgcggc cggcggcgaa accttcgacc acgttgccgc tgacgatgcg
     3901 gccatcgatc atcgggccgc tccaggtttt cttctcgtgc tcgagcaggc tgatgccgcc
     3961 cagcactttc tttgccggca gcgcgcgcag cgcagccgcg tcggcgttct tcagcccggc
     4021 atcggcggcg aatgccacgc ccgtgctctc cgccgagggc aggcccggct ggtcgtcatg
     4081 cagcttcggc cacaaggcgc ggccgccgcc ggactggctg atggccttgg caaacaagcc
     4141 gcgggcggcg tcggacagca gcagctggtg caccgagtcg ccgccggccg attcgccgaa
     4201 gatggtgacg ttggccggat cgccgccaaa cgccgcgatg ttccgctgca cccacttgag
     4261 cgccgcaatc tggtccatga aggcgtagtt gccggtggca ccgtcgccgg attcggcggt
     4321 gagcgccgga tgggcgaaga agccgaagcg cccgagacga tagttgaagg tcaccaccac
     4381 cgcgccgcgc gcggccagcc tgtcgcccag tgtgagcggc gaactgccgg cgccgctggc
     4441 gaaaccgccg ccgtgtatcc acaccattac cggcagcttc gccgccgctg tggccggcgc
     4501 ccacacgttg aggtagaggc aatcctcgct catcggctgc gccgagttgg ccttgtccca
     4561 cgacatgcgg ttctgcatgc agtcattgcc gaaggcgctg gcgtcgcgca cgccgtccca
     4621 cgcggccggc ggctgcggag cgcgccagcg caggtcaccc accggcggtg cggcgtaggg
     4681 aatgttcttg taggcgataa cgtcgccgct gcgcgcacca gacagcgcgc cggtgtcgat
     4741 cctgatcacg tcggccggcg gctgcgcctg cagcgaaccg gccagcaaca gcccggcaca
     4801 agccgaaacc acggaccacg ggcgcagggc gccatgcctg attttcatgc cacacttctc
     4861 ctgccgccgc acgttgatac cagcttccgc cacggacgga atttggttca ccacttgtat
     4921 aatgaaaaca ggatagcgca tgaagaccaa acagaaaaga gccgcgcgca ccttgaaggc
     4981 aaagccggta acgcgggccg agcagaagga agcgacgcgc gccgccatcc tgcaggcggc
     5041 gctggagatt tttgccgag
//
LOCUS       DASZZC010000749         7989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_830713,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000749 DASZZC010000000
VERSION     DASZZC010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830713"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..419)
                     /locus_tag="VHE37_15985"
     CDS             complement(93..419)
                     /locus_tag="VHE37_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37090.1"
                     /translation="MRTLAMGFTAALLLASGCATNARCKGVQDYQKAETMPAAQPVPD
                     LKVQDNASSLKIPPAPAKSVAYGEQVPDPAKPGKTRLECLDTPPAMPPETTPAPAATP
                     APARKP"
     gene            complement(416..1297)
                     /gene="dapA"
                     /locus_tag="VHE37_15990"
     CDS             complement(416..1297)
                     /gene="dapA"
                     /locus_tag="VHE37_15990"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HVT37091.1"
                     /translation="MIKGSIVALVTPMFPDGAVDWDSLAALVEWHIGEGTDAIVAVGT
                     TGESATLEVDEHIEVIRRVVKLAKGRVPVIAGTGANSTSEAIELTRAGKEAGADAALS
                     VTPYYNKPPQEGLYRHYKAIAEAVDIPLILYNVPGRTGCDMLPVTVGRLAQIKNIVGL
                     KEARGDMPRVKELLALELPRDFALYSGDDATARESILAGFHGDISVTANVAPRQMHEL
                     CVAAAKGDAARAERIDAQLAALHKDLFCEPNPIPVKWALNQMGKIPAGLRLPLVPLDA
                     KYHALVRDALRAAGAFA"
     gene            1397..1945
                     /locus_tag="VHE37_15995"
     CDS             1397..1945
                     /locus_tag="VHE37_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HVT37092.1"
                     /translation="MAATSKENNLLTISVLGRPKPQLGLELFKAIRERGGEVEDCRIA
                     QIGDRLTANLLVSGNWSTLGRLETALPGIAEKLEMQVRFERCEQRVFNPEYRPYAAEV
                     IAPQQADLLVHLLEFFQNQDVEVAEISTQKYASTYTGAEMVSVQMVLHVPVNQHPQAL
                     RESFMDLCDDLNADGMLDPIKT"
     gene            1998..2462
                     /locus_tag="VHE37_16000"
     CDS             1998..2462
                     /locus_tag="VHE37_16000"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVT37093.1"
                     /translation="MSLEPGDKIPDLTLPATGGKDISLKAQKGKKLVLYFYPKDNTPG
                     CTQEGQDFRDLHDDFIKAGAQLMGVSKDSLRSHENFAAKFKFPFPLLADVDGAACDAF
                     GTMVEKSMYGRKYIGIDRSTWLFDAKGVLRQAWRGVKVKGHAAEVLEAVQQL"
     gene            2684..4105
                     /locus_tag="VHE37_16005"
     CDS             2684..4105
                     /locus_tag="VHE37_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVT37094.1"
                     /translation="MSKRKIFVLDTNVLMHDPSSLFRFEEHDLFIPMVVVEELDAAKK
                     GTSEVSRNARQVSRFLDELMSDKDHAQIEKGLQLPAPQAGKHNGNGHAAAAGGRLFFQ
                     TRPPDASLPPLMPGNKPDNSILVTALALQSEHPGRRVTLVSKDINLRIKAAIVGVHTE
                     DYANDQVLEDADLLPVGYSTLPENFWATHSDKMRSWQDERGRACYQVRGPLVPQWRVN
                     EFLYLPDESERGLELIVQEKQGDSAQLRVIHDYRHGKSAIWGIHAKNREQNFALNLLL
                     DPEVDFVTLLGAAGTGKTLLTLAAGLAQTLDEPRYREIIMTRVTVPLGEDIGFLPGTE
                     EEKMTPWMGALMDNLEVLTQTSNAGDWERAATNDLLSKRIKIRSLNFMRGRTFLNRYI
                     IIDEAQNLTSKQMRTLITRCGPGTKMVCLGNLGQIDTPYLSETTSGLTYVVDRFQGWP
                     HGGHVTLARGERSRLAAHASDIL"
     gene            4116..4451
                     /locus_tag="VHE37_16010"
     CDS             4116..4451
                     /locus_tag="VHE37_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37095.1"
                     /translation="MSIGDAVNSLKDSAIAHALKGYINDRFGAYGEALDVEVDTRRNR
                     IQLRALLKGETQPVTASIDRYDIERDEQYTYLVLHETSASREWLSVLLSKLAEGSRHK
                     IPAAVAKFL"
     gene            4455..5216
                     /locus_tag="VHE37_16015"
     CDS             4455..5216
                     /locus_tag="VHE37_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HVT37096.1"
                     /translation="MDPVLSALCAFSFLAGFIDSVVGGGGLIQVPALFVLMPQMPAAT
                     LLGTNKFSSIWGTLSATVQYMRRTPMEWPATLPACVAAFAFSFIGARTVAHLSSDFLR
                     PLVLVMLALVLVYTLWKKDFGQLHAPRLTRSHQLITGLATGAVIGFYDGFFGPGTGSF
                     LIFAFVGLFGFSFLSASASAKAVNVTTNAAALAYFITHGNVLYATAIPMAVCNVGGAL
                     LGSHLAIRKGSGFVRALFIVIVCALIARMARDYFF"
     gene            complement(5213..6139)
                     /gene="epmA"
                     /locus_tag="VHE37_16020"
     CDS             complement(5213..6139)
                     /gene="epmA"
                     /locus_tag="VHE37_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HVT37097.1"
                     /translation="MPENNWKPSASIATLKARAELYGSVRAFFSSRGLLEVSTPLLSA
                     HATVDKHIDSFRAGDSWLHTSPEFAMKRLLAAGSGAIWQLGPVFRREEAGRFHNPEFT
                     LLEWYRPGWDHHRLMDEVDELLRTLGACARPCARLSYRDAFLRHAGLDPFAASLDDLR
                     ARTQLHELDHVDDRDTWLDLLMSHVVGPQLGRDAPEFLYDFPASQAALARVRGEVAER
                     FELFWRGIELANGFHELTDAVEQEARFRADQAWRRMQGRDVPPYDAHLIAALRAGLPT
                     CAGVALGIDRLLMLKLGLPDVASTLAFAADRA"
     gene            complement(6126..6695)
                     /gene="efp"
                     /locus_tag="VHE37_16025"
     CDS             complement(6126..6695)
                     /gene="efp"
                     /locus_tag="VHE37_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HVT37098.1"
                     /translation="MATVGTNEMKSGTKVLMDGEPYSVIDNEYRKPGKGQATNTIKVR
                     NLKNGRVIERTLKSGDTLEVADIEEVELQYLYNDGEFWHFMNPASFEQMQVGKVAMDE
                     AAKWLKGEETCKVLLFNGLPLSVTPPNTVTLKITDTDPGLRGDTSGGGGKPATLETGA
                     VVRVPLFVQLGEIIKVDTRTGEYLARAGK"
     gene            6773..7783
                     /gene="epmB"
                     /locus_tag="VHE37_16030"
     CDS             6773..7783
                     /gene="epmB"
                     /locus_tag="VHE37_16030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HVT37099.1"
                     /translation="MIPNPLPERQAPAWQHELRHAFARPLDLIDYLQLDPALPVLAAG
                     RVKDFPLRVPRGFAARMEKGNPRDPLFLQVWPQPAESDDAPGFVLDAVGDLHKLKAGG
                     IVHKYHGRVLVMATGACAVHCRYCFRRHFPYGEALAARDHWREALATIAADSSIEEVI
                     LSGGDPLSLSDDKLEEFAGALDGIAHVQRLRIHTRQPIVLPARVDERLLAWLGRGRLR
                     KVMVLHANHARELDDHVARALALLAGTGIMLLNQAVLLRGVNDNVKALNNLSVRLSAL
                     GVFPYYLHLLDRVQGAAHFEVAEGTARSLMAELARGLPGYLVPRLVREEAGAAAKTWI
                     AW"
BASE COUNT         1351 a         2654 c         2610 g         1374 t
ORIGIN      
        1 gaacccgcga tgcggcttat gaccgcatac acgctttcca ggcgtgctcc ttaaaccact
       61 cggacatctc tccggaaaaa caattttacc agttagggtt tcctggccgg cgcaggcgtc
      121 gcggccggag ccggtgtcgt ctccggcggc atcgccggcg gcgtatccag gcattccagc
      181 cgggtcttgc cgggcttggc tggatcgggc acctgttcgc cgtaggccac ggatttcgcc
      241 ggagcaggcg gaatcttcag cgaggacgcg ttgtcctgca ccttgagatc cggcaccggc
      301 tgcgcggcgg gcatggtctc ggccttctgg taatcctgca cgcccttgca gcgcgcattg
      361 gtggcgcagc cggacgcgag caacagcgcc gcggtgaagc ccattgccag tgttctcatg
      421 cgaaagctcc ggcggcgcgc agcgcgtcgc gcaccagcgc gtgatatttc gcatcgagcg
      481 gcaccagcgg caggcgcagg ccggcgggga tcttgcccat ctggttgagt gcccacttca
      541 ccgggatcgg gttgggctcg cagaacaggt ccttgtgcag cgctgccagt tgtgcgtcga
      601 tgcgctcggc gcgtgcggca tcgcccttgg ccgcggccac gcacaactcg tgcatctggc
      661 gcggcgcgac attcgcggtg acggaaatgt cgccgtggaa gccggccagg atcgattcgc
      721 gcgcggtggc gtcatcgccg gaatacagcg cgaagtcgcg cggcagttcc agcgccagca
      781 gttccttgac ccgtggcatg tcgccgcgcg cttccttgag gccgacgatg ttcttgatct
      841 gcgccagccg gccgacggtg accggcagca tgtcgcagcc ggtgcggccc ggcacgttgt
      901 acaggatcag cggaatgtcg accgcttcgg cgatggcctt gtagtggcga tacagacctt
      961 cctgcggcgg cttgttgtag tacggcgtca ccgacagcgc ggcgtcggcg cccgcttctt
     1021 tgccggcgcg cgtgagttca atggcttcgc tggtggaatt ggcgccggtg ccggcgatga
     1081 ccggcacgcg gcccttggcc agcttgacca cgcggcggat gacctcgatg tgctcgtcca
     1141 cttccagcgt ggccgattct ccggtggtgc ccacggcgac gatggcgtcg gtgccttcgc
     1201 cgatgtgcca ctcgaccagc gcggccaggc tgtcccagtc gaccgcgccg tcgggaaaca
     1261 tgggcgtgac gagcgcgacg atgctgcctt tgatcatgcg tttggatgtg cggccggaaa
     1321 gggcggcgat tatagacgtt cggcttgcac cgctgccaaa ggggattaaa cttgccggcc
     1381 tgttttgggt aagcccatgg ccgccacgtc caaggaaaac aatctcctca ccatttccgt
     1441 gctcgggcgg cccaagccgc agctcgggct ggagctgttc aaggccatcc gcgaacgtgg
     1501 cggcgaggtc gaagattgcc gtattgcgca gatcggcgac cgcctcaccg ccaacctgct
     1561 ggtgtccggc aactggagca cgctgggccg cctggaaact gcgctgcccg gcatcgccga
     1621 gaagctggag atgcaggtgc gcttcgagcg ctgcgagcag cgcgtgttca acccggaata
     1681 ccgcccctac gccgcggaag tcatcgcccc gcagcaggcc gacctgctgg tgcacctgct
     1741 ggaattcttc cagaaccagg acgtggaggt ggcggaaatt tccacgcaga aatacgccag
     1801 cacctacacc ggcgccgaga tggtcagcgt gcagatggtg ctgcacgtgc cggtgaacca
     1861 gcatccgcag gccttgcgcg aatcgttcat ggacctgtgc gacgatctga acgcggacgg
     1921 catgctcgat ccgatcaaga cctgatcgcg ccgtggcccc gcgcggcacg gcatacacaa
     1981 cttgaagaag gagcgtcatg agcctcgaac ccggcgacaa gattcccgat ctcaccctgc
     2041 ccgccaccgg cggcaaggac atcagcctca aggcgcagaa aggcaaaaag ctggtgctgt
     2101 acttctaccc caaggacaac acgccgggct gcacccagga aggccaggac ttccgcgacc
     2161 tgcacgatga cttcatcaaa gccggcgcgc agctgatggg cgtctccaag gactcgctgc
     2221 gttcgcacga gaacttcgcc gccaagttca agttcccgtt tccactgctg gccgatgtgg
     2281 acggcgcggc ctgcgacgcg ttcggcacca tggtcgagaa gagcatgtat ggccgcaagt
     2341 acatcggcat cgatcgcagc acctggctgt tcgatgccaa aggcgtgctc aggcaggcgt
     2401 ggcgcggcgt caaggtgaaa ggccacgcgg cggaagtgct ggaagcggtg cagcagctct
     2461 gagtgcagca aaaaacggcc tgaccgcacc ccgcatcggg ctgttattaa ttagtaatca
     2521 attatttttt tgatcgacac tttataaaga ccgtttgccc ctagaatcgc ggcaacgagc
     2581 gaaaaacttt gagcagcgca gaaactttcg cggtcctctt tcctgtacgc caagccggcg
     2641 cgatcccgcg ccggcttttt cgtttggagc cgatcaaacg ctaatgagca agcgcaagat
     2701 ttttgtcctc gacaccaacg tgctgatgca cgatccctcg tcgctgttcc gcttcgagga
     2761 gcatgacctg ttcattccca tggtggtggt cgaggaactc gacgccgcca agaagggcac
     2821 cagcgaagtc tcgcgcaacg cgcgccaggt cagccgcttc ctcgacgagc tgatgagcga
     2881 caaggaccac gcgcagatcg agaaaggcct gcagctgccg gcgccgcagg cgggcaagca
     2941 caacggcaac gggcatgccg ccgccgcggg cggacgcctg tttttccaga cgcgcccgcc
     3001 cgatgcctcg ctgcccccgc tgatgccggg caacaagccg gacaacagca tcctggtcac
     3061 cgcgctggcg ctgcagagcg aacacccggg gcgccgcgtg acgctggtgt ccaaggacat
     3121 caacctgcgc atcaaggcag cgattgttgg cgtgcacacc gaggactacg ccaacgacca
     3181 ggtgctggaa gacgccgacc tgctgccggt gggctattcc acgctgccgg aaaatttctg
     3241 ggccacgcac agcgacaaga tgcgctcatg gcaggacgag cgcgggcgcg cctgctacca
     3301 ggtgcgcggc ccgctggtgc cgcagtggcg cgtcaacgag ttcctctacc tgcccgacga
     3361 gtcggagcgc gggctcgagc tgatcgtgca ggagaagcag ggcgacagcg cgcagctgcg
     3421 cgtgatccac gactaccgcc acggcaagtc ggccatctgg ggcatccacg ccaagaaccg
     3481 cgagcagaac ttcgcgctga acctgctgct cgacccggaa gtggacttcg tcaccctgct
     3541 cggcgccgcc ggcaccggca agacgctgct gacgctggcc gccggcctgg cgcagacgct
     3601 ggacgagccg cgctaccgcg agatcatcat gacccgcgtg accgtgccgc tgggcgagga
     3661 catcggtttc ctgcccggca ccgaagagga gaagatgacg ccatggatgg gcgcactgat
     3721 ggacaacctg gaagtgctga cacagacctc caacgccggc gactgggagc gcgccgccac
     3781 caacgacctg ctgtccaagc gcatcaagat ccgctcgctc aacttcatgc gcgggcgcac
     3841 cttcctcaac cgctacatca tcatcgacga agcgcagaac ctgaccagca agcagatgcg
     3901 cacgctgatc acgcgctgcg gccccggcac caagatggtg tgcctgggca acctcggcca
     3961 gatcgacacg ccgtacctgt cggagaccac ctcgggcctg acctacgtgg tcgaccgttt
     4021 ccagggctgg ccgcatggcg gccacgtcac cctggcgcgc ggcgaacgct cacgcctggc
     4081 ggcgcacgcc tccgacatcc tgtgagcgtg tcgccgtgag catcggcgac gcggtcaaca
     4141 gcctgaagga cagcgccatc gcgcacgcgc tcaagggcta catcaacgac cgcttcggcg
     4201 cgtacggcga ggcgctggat gtggaagtgg acacgcgccg caaccgcatc cagttgcgcg
     4261 cgctgctcaa gggcgaaaca cagccggtga cggcgtcgat cgaccgctac gacatcgagc
     4321 gcgacgagca gtacacctac ctggtgctgc acgaaacctc ggcctcgcgc gaatggctgt
     4381 cggtattgct gagcaagctg gccgagggca gccggcacaa gatcccggcg gcggtggcga
     4441 agttcctgta gcccttggac ccagtcctca gcgccctctg cgccttctcc ttcctcgccg
     4501 gcttcatcga ctcggtggtc ggcggtggcg ggctgatcca ggtgccggcg ctgttcgtgc
     4561 tgatgccgca gatgccggcg gccaccctgc tgggcaccaa caaattttcc agcatctggg
     4621 gcacgctgtc cgccaccgtg cagtacatgc ggcgtacacc gatggaatgg cccgccactc
     4681 tgcccgcctg cgtcgcggcg ttcgccttca gcttcatcgg cgcgcgcacg gtggcgcacc
     4741 tgtccagtga tttcctgcgg ccgctggtgc tggtcatgct ggcgctggtg ctggtctaca
     4801 cgctgtggaa gaaggatttc gggcagctgc acgcaccgcg actgacgcgc tcgcaccagt
     4861 tgatcaccgg tctggcaacc ggcgccgtca tcggcttcta cgacggcttc ttcggtccgg
     4921 gcacgggttc gttcctgatc ttcgccttcg tcggcctgtt cggcttctcg ttcctgtcgg
     4981 cctcggcttc ggccaaggcg gtgaacgtga ccaccaacgc ggcggcgctg gcctacttca
     5041 tcacccatgg caacgtgctg tacgccaccg cgatcccgat ggcggtgtgc aacgtcggcg
     5101 gcgcgctgct cggctcgcac ctggccatcc gcaagggcag cggcttcgtg cgcgcgctgt
     5161 tcatcgtcat cgtctgcgcg ctgatcgcca ggatggcgcg cgactatttc ttctaggcgc
     5221 ggtcggcggc gaaggccagc gtgctggcca cgtcgggcaa tcccagcttc agcatcagca
     5281 gccggtcgat gcccagcgcg acgcccgcgc acgtgggcag gccggcacgc agcgccgcga
     5341 tgagatgagc gtcgtacggc ggcacgtccc tgccctgcat gcggcgccac gcctggtccg
     5401 cgcggaagcg cgcttcctgt tcaaccgcgt cggtgagttc gtggaagcca ttggccagct
     5461 cgatgccgcg ccagaacagc tcgaatcgtt cggcgacttc gccccgcacg cgcgccagcg
     5521 ctgcctgcga ggccggaaaa tcgtagagga attccggagc gtcgcggccg agctgcggcc
     5581 cgaccacgtg cgacatcagc aggtccagcc aggtgtcgcg gtcgtcgaca tgatccagtt
     5641 cgtgcagctg cgtccgtgca cgcaggtcgt ccaggctggc ggcgaacgga tcgagcccgg
     5701 catggcgcag gaaggcatcg cggtagctca gccgcgcaca tggccgggcg cacgcgccga
     5761 gggtgcgcag caattcgtcg acctcgtcca tcaggcggtg gtggtcccag cctggccggt
     5821 accactccag cagggtgaac tcgggattgt ggaagcggcc cgcttcctcg cggcggaata
     5881 ccgggcccag ctgccagatg gcgccgctgc cagccgcgag cagtcgcttc atggcgaatt
     5941 ccggcgaggt atgcagccag gagtcgccgg cgcggaaact gtcgatatgc ttgtcgaccg
     6001 tggcatgcgc cgacagcagc ggcgtgctca cttcgagcaa tccgcgcgat gaaaaaaaag
     6061 cgcggacgga accgtaaagt tccgcccgcg ctttcagcgt tgcaatgctt gcgctgggtt
     6121 tccagttatt ttccggcacg agcgagatac tcgccggtgc gcgtgtcgac cttgatgatc
     6181 tcgcccagct gcacgaacag cggcacgcgc accacggcgc cggtttccag cgtggccggc
     6241 ttgccgccgc cgccgctggt atcgccgcgc aggccggggt cggtgtcggt gatcttgagg
     6301 gtgacggtgt ttggcggcgt gaccgacagc ggcaggccgt tgaacagcag caccttgcag
     6361 gtctcctcgc ccttgagcca cttggcggct tcgtccatcg ccaccttgcc cacctgcatc
     6421 tgctcgaagc tggccgggtt catgaagtgc cagaactcgc catcgttgta caggtactgc
     6481 agttccactt cctcgatgtc ggccacttcc agcgtgtcgc cggacttgag cgtgcgctcg
     6541 atgacgcggc cgttcttcag gttgcgcacc ttgatggtgt tggtggcctg gcccttgccg
     6601 ggtttgcggt actcgttgtc gattaccgag tacggttcgc cgtccatcag caccttggtg
     6661 ccggacttca tttcgttggt gccgacggtt gccatgttta cgctctacgc ttttgttgaa
     6721 agtcgcggcc atggatggcc gcgccggtga caggatgtca aaatcggcgc gcatgatacc
     6781 caatcccctt cccgagcggc aggctccggc ctggcagcac gagctgcggc atgcctttgc
     6841 ccgcccgctg gacctgatcg actacctgca attggacccg gcgctgccgg tgctggcggc
     6901 cgggcgggtg aaggatttcc cgctgcgcgt gccgcgcgga ttcgccgcgc gcatggagaa
     6961 aggcaacccg cgcgatccgc tgttcctgca ggtgtggccg cagccggcgg agagcgacga
     7021 cgcgccgggt ttcgtgctcg acgcggtggg cgacctgcac aagctcaagg ccggcggcat
     7081 cgtgcacaag taccacggcc gcgtgctggt gatggccacc ggcgcctgcg cggtgcactg
     7141 ccgctactgc ttccgccggc atttccccta cggcgaggcg ctggccgcgc gcgaccattg
     7201 gcgcgaggcg ctggccacca tcgcggccga ttccagcatc gaggaagtga ttctctccgg
     7261 cggcgacccg ctgtcgctgt cggacgacaa gctggaggaa ttcgccggcg cgctcgacgg
     7321 catcgcccac gtgcagcgcc tgcgcatcca cacgcgccag cccatcgtgc tgcccgcgcg
     7381 ggtggacgag cgcctgctgg cctggctcgg gcgcggccgg ctgcgcaagg tcatggtgct
     7441 gcacgccaac cacgcccgcg aactcgatga ccacgtcgcc cgcgcgctgg cgctgctggc
     7501 cggcaccggc atcatgctgc tcaaccaggc cgtcctgctg cgcggcgtca acgacaacgt
     7561 aaaggcatta aacaatttgt ccgtgcgcct gtccgcgctg ggggttttcc cctactatct
     7621 gcatctgctg gaccgcgtcc agggcgctgc gcatttcgag gttgcggaag gcacggcgag
     7681 gagcctgatg gctgaactgg cgcgcggcct gcccggttac ctggtaccgc gcctggtcag
     7741 ggaagaagca ggcgccgctg caaaaacatg gatcgcatgg tgaacagtcc gccacagaga
     7801 atcgctccgc acgccgggtc ggcacagacc tcggtgatgc tggtggtgag cgagtctgag
     7861 aacgtggcca agcgcatcga aagccacctg cgcaacgccg cgcacgccgt gcgcgtggcc
     7921 tgggtcaccg acctggaaga catcgaggac gcgctgcgcc gcggcgcgcc cgacctggtg
     7981 ctgtgcgaa
//
LOCUS       DASZZC010000750         6551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_184042,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000750 DASZZC010000000
VERSION     DASZZC010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184042"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VHE37_16035"
     CDS             complement(<1..274)
                     /locus_tag="VHE37_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37100.1"
                     /translation="MLIAWLLASPVMRALDPSALFMLRPMSALLILVTALALAGCASS
                     WNSRAASILCLLGALLAAMLMGESILNYDRGIELLISPALDSDGAAS"
     gene            complement(488..913)
                     /locus_tag="VHE37_16040"
     CDS             complement(488..913)
                     /locus_tag="VHE37_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVT37101.1"
                     /translation="MEEMLESGHVPHTVGDLMTRSMITVTDDETVEQAMHVMTENNVS
                     SVVVEPNDKGEWGVITRRDIVTKIVRVNKSPATAKVKDVASRPLFSVPAETSIREAAN
                     ILAEKNFSRLTISQGGKVIGIVTETDIFNAVEKYGWAAE"
     gene            complement(1010..2248)
                     /gene="icd"
                     /locus_tag="VHE37_16045"
                     /pseudo
     CDS             complement(1010..2248)
                     /gene="icd"
                     /locus_tag="VHE37_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
     gene            2353..2895
                     /locus_tag="VHE37_16050"
     CDS             2353..2895
                     /locus_tag="VHE37_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HVT37102.1"
                     /translation="MKALIPIAHGSESLETVTLANVLRRGGVEVTLASIESSRAVQAT
                     RGIVLTADASWDEVAARDYNLIALPGGEQGAAALGRHEGLIEKLRRQRNGHKWYGAIC
                     AAPALVLSPHGLLDGKQATCYPAFRDKLIHFVNLPVVVDGHCVTSQGPATALAFGLKL
                     VEVLCGEAKAREVAQQALAA"
     gene            complement(2959..3288)
                     /gene="grxD"
                     /locus_tag="VHE37_16055"
     CDS             complement(2959..3288)
                     /gene="grxD"
                     /locus_tag="VHE37_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HVT37103.1"
                     /translation="MDALERIKQQVTDNPVMIYMKGSPDFPQCGFSARAVGALKACNV
                     PFAYVNIFEDEEIYRALPKFANWPTFPQLYVKGELVGGCDITLDLFQSGELQKMLDEA
                     VGAKKAA"
     gene            3467..4042
                     /locus_tag="VHE37_16060"
     CDS             3467..4042
                     /locus_tag="VHE37_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HVT37104.1"
                     /translation="MALTFPDLPWPREALEPHMSRETLDYHYGKHHKAYVDTGNKLIA
                     GTEFENMAIEDIIKKSSGKIFNNAAQVWNHTFFWNCLTPKAAAPGKKITDALVKQFGG
                     LDDFKKQFTETAMGTFGSGWAWLVKNADGSLAIVSTTGAGNPMTAGQKPLLTCDVWEH
                     AYYIDYRNARATFLEHFWALVNWSFVEKNLG"
     gene            4116..5012
                     /gene="argF"
                     /locus_tag="VHE37_16065"
     CDS             4116..5012
                     /gene="argF"
                     /locus_tag="VHE37_16065"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HVT37105.1"
                     /translation="MGSVKHFLKLSDLSASEAQAVVARASWLKQHWTPAHRPFVGRTL
                     AMLFTKNSTRTRVSFESGMAKFGGHALFLSAGATQIGRDEPIADTARVLSRMCDAIMI
                     RNDSQADQQALAANSRVPVINGLSDEHHPCQLLADMQTWIEHRGDPAGKTVAWIGDGN
                     NVCHSLIEAAGLFGFSLRIACPGGYEPSTDIAVNDTEVNLVSSAAAAAEGADLVVTDT
                     WTSMGQEAEQAKRVAAFAPYQVDAALMKRARPDALFMHCLPAHRGEEVSAEVIDGPQS
                     VVWDEAGNRMWAQLALLEFLLC"
     gene            5148..6002
                     /gene="nadC"
                     /locus_tag="VHE37_16070"
     CDS             5148..6002
                     /gene="nadC"
                     /locus_tag="VHE37_16070"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HVT37106.1"
                     /translation="MPAPTAPADLADCVRRALAEDIGSGDITAQLVPAHERAQARVVA
                     RERAVVCGRPWVDEVFRQLDASIKLDWKFGESGRCDADDVLFTLSGPARALLTGERCA
                     LNFLQTLSGTATATRAFVDAVAGTGCAIVETRKTLPGLRTAQKYAVVCGGGVNNRIGL
                     YDAILIKENHIAAAGGIAQAIAQARALKAGVPLMTEAETLDECRAALAADVDLVLVDE
                     FPPPELREAVALTRAHRARGGRTLIEYSGGTTLANVRALAESGVDRISVGSITKHVHA
                     VDLSMRFF"
     gene            complement(6135..>6551)
                     /locus_tag="VHE37_16075"
     CDS             complement(6135..>6551)
                     /locus_tag="VHE37_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HVT37107.1"
                     /translation="GAALAGEKFHHLLAAHARNVRVITRPCAGWVELVERGEVSGAQA
                     QSLVEREVSPLLAQGADVLVLGCTHYPFLRPLVERAAGPAVTVLDTGAAVARHTRRVL
                     EREQLRSMQARNGSIEWHTSGDARQFASLRERLLQV"
BASE COUNT         1067 a         2219 c         2115 g         1150 t
ORIGIN      
        1 aggatgccgc gccatcgctg tcgagcgcgg gggaaatcag cagttcgatg ccgcggtcgt
       61 aattgaggat gctctcgccc atcagcatgg ccgccagcag ggcgcccaag aggcaaagga
      121 tactggccgc acggctgttc cagcttgaag cgcacccggc cagcgccagc gccgtcacca
      181 ggatcagcag ggcggacatc gggcgcagca tgaacagcgc cgacgggtcc agcgcccgca
      241 ttaccgggct ggccaacagc caggcgatca gcaccaggga agcgtacgag aacacgctcc
      301 acgcgcactg cttggcaaac cggtgtccca tggcgagccg ctgggcctcc ccctcctcgt
      361 tgctccctct ccgcccccca ggcgcgatcg ggtccgcaca gcatactgcg gcgggcgcgc
      421 ctgcgcccgc ggttccccgc ccagtggctg gctcagcggt cccggtccgg gcgccgccgg
      481 cttgtggcta ttctgctgcc cagccgtatt tttcgacggc gttgaagata tccgtctcgg
      541 taacgatgcc gatgaccttg ccgccctggc tgatggtcag gcgtgaaaaa ttcttctcgg
      601 caagaatatt ggcggcctcg cggatgctgg tttcggccgg caccgaaaac agcggccggc
      661 tggccacatc cttgaccttg gccgtggcgg gcgatttgtt cacgcgcacg atcttggtga
      721 cgatgtcgcg ccgtgtgatc acaccccact cgcccttgtc gttgggttcc accaccaccg
      781 acgacacgtt gttttcggtc atcacgtgca tggcctgctc gacggtctca tcgtcggtca
      841 ccgtgatcat cgagcgggtc atcaggtcgc ccaccgtgtg cggcacgtgg ccggactcga
      901 gcatctcttc catcggctgc agcaggcggc ggatctcgcg ctgtttgcgt tccttggcca
      961 gcaggtcggc ctgcgccttg cgcttggcct cgagatcgat gccgccgttg atctgctcga
     1021 tcagggcatc ggcgaactga ctggtgccga cttccttggc gccgtcgatc tggcgggcga
     1081 aatcgtaggt gacgatgcca tcggcgatca cttccggata tgccagggtg atcaggtcgg
     1141 cagcctcggt ccagcccatg tactccagca tcatcacccc ggagaacagc agcgagcccg
     1201 ggttgacctt gttgaggttc gcgtacttgg gtgcagtgcc atgggtggcc tcgaacaccg
     1261 ccacgtgatc ggcgatgttg gcgccggggg cgatgcccat gccaccgact tccgccgcga
     1321 tggcgtcaga caggtaatcg ccgttgaggt tgggcgtggc cagcacgtcg aactcgtcgg
     1381 ggcgcagcag catcatctgg aacatgatgt cggcgatgcg atccttgatg acgatcttgc
     1441 cctcgggctg cttgcccttg tactcggcgt acagctgctc ctcggtgatg gtgacgctgc
     1501 cgaactcctc gcgcgcaact tcatatcccc agttgcggaa ggcgccctcg gtgaacttca
     1561 tgatgttgcc cttgtgcacc agggtcaccg actcgcggcc attgtcgatc gcgtactgga
     1621 tggccttgcg gatgagccgc ttggagccga actccgagat cggcttgatg cccaggcccg
     1681 cgccctcgaa gaacttggcc ttcatctcct cgcgcaggaa cttggcgacc ttttcattct
     1741 ccggcgtgcc ggacttgtat tcgatgcccg cgtacacgtc ttcagtgttc tcgcggaaga
     1801 tcaccacgtc gaccttgccc ggctgcttca gcgggctggg gacgccggcg taatggcgca
     1861 ccgggcgcac gcaggcgtag aggtcgagat cctggcgcag cgccacgttg agcgagcgga
     1921 agccgccgcc caccggcgtg gtcagcgggc ccttgatcga gatgaccagg tcctgcagca
     1981 ccttgagcgt ttcgtccggg cagtagttgc cgtcgtacag gccggcggcc ttctcgccga
     2041 ggaaggtttc gcaccagtgg atcttgcgct tgccgccgta agccttggcc accgccgcat
     2101 cccagatgcg caggcaggcc ttggtgatgt caggcccgat gccgtcgcct tccacgtaac
     2161 cgatgatcgg ctggtccggc acgatgcgct tgccgttgac cacgcgaacc ttttcaccgg
     2221 atgtgggaat cttgatgtgg cgatacatgt cgcgtcctct ttttatgggg cttgggcggg
     2281 cgcggattgt agcaccggcc tgttgtgcgg ccctgttaca gtgatggccg gttcgcgcag
     2341 acggagcagc ggatgaaagc cttgattccc atcgcccacg gctcggaaag cctggaaacc
     2401 gtgaccctgg ccaacgtgct gcggcgcggc ggcgtcgaag tcacgctggc cagcatcgaa
     2461 agcagccgcg cggtgcaggc cacgcgcggc atcgtgctga ccgctgacgc gtcgtgggac
     2521 gaggttgccg cccgcgacta caacctgatc gccctgcctg gcggcgagca gggagccgcc
     2581 gccctcggcc gccacgaagg cctcatcgaa aagctgcggc gccagcgcaa cggccacaag
     2641 tggtatggcg ccatctgcgc agcgccggcg ctggtcctgt cgccgcacgg cctgctcgat
     2701 ggcaagcagg ccacctgcta cccagcgttc cgcgacaagc tgatccactt cgtcaacctg
     2761 ccggtggtgg tcgacggcca ctgcgtcacc agccagggtc cggccacggc gctggccttc
     2821 ggcctcaagc tggtggaagt gctgtgcggg gaggccaagg cccgcgaagt ggcgcagcag
     2881 gcgctcgccg cctgagtttg cgcgggcgaa acaaaaacgc ccgcttgcgc gggcgtggtg
     2941 acttgcagct gaagcggatc aggcggcttt cttggcaccc accgcttcgt cgagcatctt
     3001 ctgcagctcg ccggactgga agagatccag cgtgatgtcg cagccgccca ccagctctcc
     3061 cttcacgtac agctgcggaa acgtcggcca gttggcgaac ttcggcagcg cgcggtagat
     3121 ctcctcgtcc tcgaagatgt tgacgtaggc gaacggcacg ttgcacgcct tcagcgcgcc
     3181 gaccgcgcgc gcggaaaatc cgcattgcgg aaaatccggc gagcccttca tgtaaatcat
     3241 caccgggttg tcggtgacct gctgcttgat acgctcgagc gcgtccatat ttttccctcc
     3301 acgcccctgc gggcggtgct cctgaagatt cagcgggcgc ggattatacg cgccgcgcga
     3361 ttcgacacta gtgacaagtt gaaattcccg aaccgctcgc ctattttgag cgtcctttcg
     3421 acgccaccct gcgcgaaaac gagtcgctca acaagaggaa aatcccatgg ccctgacctt
     3481 ccctgacctg ccctggccgc gcgaagcgct ggagccgcac atgtcgcgcg agacgctcga
     3541 ctatcactac ggcaagcacc acaaggcgta cgtcgatacc ggcaacaagc tgatcgccgg
     3601 taccgagttc gagaacatgg cgatcgagga catcatcaag aagtcctccg gcaagatttt
     3661 caacaacgcc gcgcaggtgt ggaaccacac cttcttctgg aactgcctga cgcccaaggc
     3721 ggccgcaccg ggcaagaaga tcaccgacgc gctggtcaag cagttcggcg ggctggacga
     3781 cttcaagaag cagttcaccg aaaccgccat gggcaccttc ggctccggct gggcctggct
     3841 ggtgaagaac gccgacggct cgctggccat cgtcagcacc accggcgctg gcaacccgat
     3901 gaccgcgggg cagaagccgc tgctgacctg cgatgtgtgg gagcacgcct actacatcga
     3961 ctaccgtaat gcccgcgcca ccttcctcga gcatttctgg gcgctggtca actggagctt
     4021 cgtcgagaag aatctcggct gatccgcacg cacacgcgtt ggcgacaagc ggggccgcgc
     4081 ggctggcgcg gccccttttc tttttggggt gaggcatggg tagcgtgaaa catttcctga
     4141 agctgtccga cctgtccgcc agcgaagcgc aggcggtggt cgcgcgcgcc agctggctga
     4201 aacagcactg gacgccggcg caccggccct tcgtcggccg caccctggcc atgctgttca
     4261 ccaagaacag cacgcgcacg cgcgtgtcgt tcgagtcggg catggcgaag ttcggcggcc
     4321 atgcgctgtt cctgtccgcc ggcgccaccc agatcgggcg cgacgagccc atcgccgaca
     4381 ccgcgcgcgt gctgtcgcgc atgtgcgacg ccatcatgat ccgcaacgat tcgcaggccg
     4441 accagcaggc gctggccgcc aactcgcgcg tgccggtgat caacggactg tccgacgagc
     4501 atcacccgtg ccagctgctg gccgacatgc agacctggat cgagcaccgc ggcgatcccg
     4561 ccggcaagac cgtggcctgg atcggtgacg gcaacaacgt gtgccactcg ctgatcgagg
     4621 cggccgggct gttcggcttc tcgctgcgca tcgcctgccc gggcggctac gagccgagca
     4681 ccgacatcgc ggtcaacgac accgaggtca acctggtcag cagcgcggcc gctgccgctg
     4741 aaggtgccga cctggtggtc accgacacct ggaccagcat gggccaggag gccgagcagg
     4801 ccaagcgcgt cgcggcattc gcgccctacc aggttgacgc cgcgctgatg aagcgcgcgc
     4861 ggccggatgc tttgttcatg cactgcctgc cggcgcaccg cggcgaggaa gtcagcgccg
     4921 aggtgatcga cggcccgcag tccgtggtgt gggatgaagc cggcaaccgc atgtgggcgc
     4981 aattggcgct gcttgagttc ctgctgtgct gaacttcggc gccagcgcca attgccggca
     5041 gctcccgcac ccacgctcgt cctcgctggt gcgagggctc accgcggcgt gtcgcggccg
     5101 tgttccggcc cttctcgaat tcctgttgct gtcagcctga ggcccgcatg cccgcaccta
     5161 ccgcacctgc agatctcgcc gattgcgtgc gccgcgcgct ggccgaggac atcggcagcg
     5221 gcgacatcac cgcccagctg gtgccggcgc acgagcgcgc gcaggcccgc gtggtcgcgc
     5281 gcgagcgcgc cgtggtgtgc ggacgcccgt gggtggacga ggtgttccgc cagctcgacg
     5341 cgtcgatcaa gctcgactgg aagttcggcg agagcggacg ctgcgatgcc gacgatgtgc
     5401 tgttcacgct gtccggtccg gcgcgcgcgc tgctcaccgg cgagcgctgc gcgctcaact
     5461 tcctgcagac gctgtcgggc accgccaccg ccacgcgtgc cttcgtcgac gcggtggccg
     5521 gcaccggctg cgccatcgtc gagacgcgca agaccctgcc cggactgcgc accgcgcaga
     5581 aatacgcggt ggtgtgcggt ggcggcgtca ataaccgcat cggcctgtac gacgcgatcc
     5641 tcatcaagga aaaccacatc gccgcggcgg gcggcatcgc gcaggccatc gcgcaggcgc
     5701 gtgcgctcaa ggcgggcgtg ccgctgatga ccgaggccga gacgctggac gaatgccgcg
     5761 cggcgctcgc cgccgatgtc gacctggtgc tggtcgacga atttccgccg cccgaattgc
     5821 gcgaggcggt ggcgctgacg cgcgcgcatc gcgcacgcgg cggacgcacg ctgatcgaat
     5881 actccggcgg caccacgctg gccaacgtgc gcgcgctggc cgagagcggc gtcgaccgca
     5941 tctcggtggg cagcatcacc aagcacgtgc atgcggtgga cctgtcgatg cgcttcttct
     6001 agccgcggcg cggcgcgttt tttcctctca ccgcacccgc atttctgctg ttatcaatta
     6061 ttgtctatat ttacaatcag acaatactta ataacacgcc agcaggcggt gcggtgccgt
     6121 ctttttttgc cccttcagac ctgtagcagg cgctcgcgca agctggcgaa ctgccgcgcg
     6181 tcgccgctgg tgtgccattc gatgctgccg ttgcgcgcct gcatcgaccg caactgctcg
     6241 cgctccagca cgcgccgcgt gtggcgcgcc accgcggcgc cggtgtcgag caccgtcacc
     6301 gcggggccgg ccgcgcgttc caccagcgga cgcaggaacg ggtagtgcgt gcagcccaga
     6361 accagcacgt cggcgccttg cgccagcagt ggtgacactt cgcgttcgac caatgattgc
     6421 gcctgcgcgc cgctgacttc gccgcgctcc accagctcga cccagcccgc gcaggggcgc
     6481 gtgatcacac gcacgttgcg cgcgtgcgcc gccagcaggt gatggaattt ttctcccgcc
     6541 agcgccgcgc c
//
LOCUS       DASZZC010000751         6305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_119282,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000751 DASZZC010000000
VERSION     DASZZC010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119282"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VHE37_16080"
     CDS             <1..558
                     /locus_tag="VHE37_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT37108.1"
                     /translation="KMFACEWAKVSPDLMCLSKGLTGGTLPLSVTLAREDIYQAFYAE
                     HAAGKAFLHSHSFSGNALGCAAALATLDLFAQFDVMSSNRKLAQLMWEGTGELRAHPR
                     VAEVRQQGMILAIELAQNARTRAPYPAHERRGLRAYRHALERGVILRPLGDIIYFMPP
                     YVITPKEVALMCEVATEAIDHATRG"
     gene            646..1377
                     /locus_tag="VHE37_16085"
     CDS             646..1377
                     /locus_tag="VHE37_16085"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HVT37109.1"
                     /translation="MTRIYIPAELKPGTITELPEGAARHLVAVLRMQAGEEFVAFNGH
                     GGEYELRLEHAGKRGASARVLTYSERERESKLHITLAQCVSKGERMDYTLQKAVELGV
                     SAIVPLLSSRSVVRMDGERWEKKLEHWRGVIVSACEQSGRTRIPPLAGVSEIEKWLPT
                     APKDALRLTLAPGPHGALHEIEHRADQQVVLLVGPEGGLSANEIADAEESGFQCVRLG
                     PRVLRTETAALVCLSNIQALWGDLR"
     gene            1395..2717
                     /locus_tag="VHE37_16090"
     CDS             1395..2717
                     /locus_tag="VHE37_16090"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVT37110.1"
                     /translation="MLIKNAHIINEGREIQGDLLIRNGRIEKVGGHIPAEGNVEVIDA
                     HGHWLLPGMIDDQVHFREPGLTHKGDLATESAAAVAGGITSYFDMPNNSPPITTRALL
                     REKYERAAGRSHANYAFYFGGANDNLEEVAALQKDEACALKVFMGASTGNMLVDDPKT
                     LDGIFARCPLMVVTHCEDTPTIKANEEKFRAKYGDDVPYSMHPLIRSEEACYKSTLLA
                     TTLAKKHGARLHVLHLTTARELEFFTPGPLKDKRITVEACVHHLYMDESLYDSRGAQI
                     KCNPAIKTAADRKALVAAVREDRIDVIATDHAPHTKQEKAQGYWKSPSGLPLVQHALL
                     MLIDLVKRGELNLAQVVQKVCHAPAELFGVRERGYIREGSFADLALIDPRESTTVTAD
                     HVLYKVGWSPLEGVTLPARIEATWVNGELAYRDGRVIPRALGQRLHFS"
     gene            2948..4378
                     /locus_tag="VHE37_16095"
     CDS             2948..4378
                     /locus_tag="VHE37_16095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HVT37111.1"
                     /translation="MQQLSGLDAVFLNLETNAVPMHIGSLIILDPTTAGPGFGFARVR
                     QLVAERLHLLPGFRRRLLSTPWNLDQPYWLEDPDFDIENHVRHRALPAPGNAQQLSEF
                     ICDIFATRLDRERPLWVFYYIEGLEGGKVACLSKIHHACIDGLSGAELLSNLLDTTPQ
                     GREVPPPEAPWMPDEIPTPWKLTYTTAKNVLSRPGEIYRLARETLPLLMSARKEMLAQ
                     QHAAEKSATPTSGGAPRMRFNTTITSRRSYAFRTVALADIKLIKNAFGVSVNDVVLTV
                     CAEALRNYLLEKKELPRQPLVAAIPVSVRSGDERGAGGNRVTMARAPLGTHIADPVER
                     LLAMSRKMGDVKKTMKAIPARLMLDWADAPPPAVMAQAARLYENFSIQNYMSPPFNLV
                     ISNVPGPPGALYFAGARVEANFPVSIPYHGLACNITVFSLQDNLDIGITAHRGTVPDI
                     DHLMDLLVDAVAVLKQRAGAQLKKAS"
     gene            4385..5308
                     /locus_tag="VHE37_16100"
     CDS             4385..5308
                     /locus_tag="VHE37_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT37112.1"
                     /translation="MADLDRLIQTLYASALETDREQFRPLALKEVCQWLGATGAAWLT
                     RSVTGLPGEYTECPEAGISIEQLSRLNFSDGVREYVLDPLPPELSPGGKASGQEQGYL
                     MHYVHRGGGLNSTVLLRAPRGRKLQHQGDLRRAIGHLVEAGTLSLRQYIHRDEKLYEL
                     GRASRGTAALVDAQGTIYAASTRFRELLSGEFGDHNFTALPQPLPEEAHTGEGLFFQG
                     ALHFRSSKKGRLFLLHARRPLPLDGLSPREQQIARALGTGKTFKSVARQFGIAVSTVA
                     NHASRIYRKLGIFRREELVEMVRTPTSNDSE"
     gene            5357..>6305
                     /locus_tag="VHE37_16105"
     CDS             5357..>6305
                     /locus_tag="VHE37_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HVT37113.1"
                     /translation="MAETMEFAYQLIFAAGLLFLVSIVATTLTPRLGVPLLLVFIVIG
                     MLAGEDGPGGIHFDDLRLTNLASSAALAVILFDGGMRTSIRTFRVGLWPAISLATIGV
                     GLTTLVVGWCGHKLLGLPLPVALLLGAIVSSTDAAAVFSLLHYRSVALNERVTSVLEI
                     ESGTNDPMAVFLTLALLSYIAAPADYGAGDFLMQFLAQAGIGGALGAAGGWLLVRALN
                     KLELSEPLYPVAALFSALLLFGLTALLDGSGFLAVYLAGLIVGNRRVRAMASIRRFHD
                     AIAWMAQIGMFLILGLLAAPHRLAEVALPALLLGLVLIFA"
BASE COUNT         1079 a         2175 c         2080 g          971 t
ORIGIN      
        1 aagatgtttg cctgcgagtg ggcaaaagtt tcgccggacc tgatgtgcct gtccaagggc
       61 ctcaccggcg gcacactgcc gctgtcggtc acgctggcac gcgaagacat ctaccaggcg
      121 ttctacgcgg agcacgccgc cggcaaggcg tttctgcact cgcattcctt cagcggcaac
      181 gcgctgggct gcgcagcggc gctggccacg ctggacctgt tcgcgcaatt cgacgtgatg
      241 tcgtcgaacc gcaagctggc gcagctgatg tgggaaggca ccggcgagct gcgcgcgcat
      301 ccacgcgtgg ccgaggtgcg gcagcagggc atgatcctgg cgatcgagct cgcgcagaac
      361 gcacgcacgc gcgcgcccta cccggcgcac gagcgccgcg ggctgcgcgc gtaccggcat
      421 gcactggagc gcggcgtgat cctgcgcccg ctcggtgaca tcatctactt catgccgccc
      481 tacgtgatca cgccgaagga agtcgcgctc atgtgcgaag tggcgacgga ggccatcgat
      541 catgcaaccc gtggctagcc gtcattcccg cgcaggcggg aatccagttc ctgcttgcag
      601 gagaaaactg ggtcaccgcc gtcgcggtga cgaccagagt gcaacatgac ccgcatctac
      661 atccccgccg agctgaagcc cggaaccatc accgaactgc ccgagggcgc cgcgcgccac
      721 ctggttgccg tgctgcgcat gcaggccggc gaggagttcg tcgccttcaa cggccacggc
      781 ggcgagtacg aactgcggct ggagcacgcg ggcaagcgcg gcgccagcgc acgcgtgctg
      841 acgtacagcg agcgcgagcg cgaatcgaag ctgcacatca cgctggcgca atgcgtgtcc
      901 aagggcgagc gcatggacta cacgctgcag aaagcagtgg aactcggtgt ctccgccatc
      961 gtgccgctgc tgtcctcgcg cagcgtggtg cgcatggacg gcgagcgctg ggagaagaaa
     1021 ctcgagcact ggcgcggcgt catcgtctcc gcctgcgagc agtccgggcg cacgcgcatt
     1081 ccgccgctgg ccggcgtcag cgagatcgag aaatggctgc caacggcgcc gaaagacgcg
     1141 ctgcgcctga cgctggcacc cggcccgcat ggcgccctgc acgagatcga gcaccgcgcc
     1201 gaccagcagg tggtgctgct ggtcggcccg gaaggcgggt tgtcggccaa cgagatcgcc
     1261 gacgccgagg aaagcggctt ccagtgcgtg cgcctgggcc cgcgcgtgct gcgcacggaa
     1321 accgcggcgc tcgtatgctt gtcgaacatc caggcgctgt ggggcgactt gcgctgatac
     1381 caccgggaac aacaatgctg atcaagaacg cgcacatcat caacgagggc cgcgagatcc
     1441 agggcgacct gctgatcagg aacggccgca tcgagaaggt gggcgggcac atccccgccg
     1501 agggcaacgt ggaagtgatc gacgcgcacg gccactggct gctgccgggc atgatcgacg
     1561 accaggtgca tttccgcgag ccgggactga cgcacaaggg cgaccttgcc accgagagcg
     1621 cggcggcggt ggccggcggc atcaccagct atttcgacat gcccaacaac agcccgccga
     1681 tcaccacgcg cgcgctgctg cgcgagaagt acgagcgcgc ggccgggcgc tcccacgcca
     1741 actacgcgtt ctacttcggc ggcgccaacg acaacctcga ggaagtggcg gcgctgcaga
     1801 aggacgaggc ctgcgcgctc aaggtgttca tgggcgcatc caccggcaac atgctggtgg
     1861 acgatccgaa gacgctcgac ggcatcttcg cgcgctgccc gctgatggtg gtgacgcact
     1921 gcgaggacac gccgaccatc aaggccaacg aggagaaatt ccgcgccaag tacggcgacg
     1981 acgtgccgta ctcgatgcac ccgctgatcc gctcggaaga ggcctgctac aagtccacgc
     2041 tgctcgccac cacgctggcc aagaaacacg gcgcgcgcct gcacgtgctg cacctgacca
     2101 ccgcgcgcga gctggagttc ttcacgcccg gtccgctgaa ggacaagcgc atcacggtcg
     2161 aggcctgcgt gcatcacctg tacatggatg agtcgctcta cgactcgcgc ggcgcgcaga
     2221 tcaagtgcaa cccggcgatc aagaccgcgg ccgaccgcaa ggcgctggtg gccgcggtgc
     2281 gcgaggaccg catcgacgtc atcgccaccg accacgcgcc gcacaccaaa caggaaaagg
     2341 cgcagggcta ctggaagtcg ccgtcgggac tgccgctggt gcagcacgcg ctgctgatgc
     2401 tgatcgacct ggtcaagcgc ggcgagctga acctggcgca ggtggtgcag aaggtctgcc
     2461 atgcaccggc cgagttgttc ggcgtgcgcg agcgcggcta catccgtgaa ggctctttcg
     2521 ccgacctggc gctgatcgat ccgcgcgaga gcaccacggt gaccgccgac cacgtgctgt
     2581 acaaggtggg ctggtcgccg ctggaaggcg tcacgctgcc ggcgcgcatc gaggcgacat
     2641 gggtgaacgg cgaactggcc taccgcgacg gccgcgtgat tccgcgcgcg ctgggccagc
     2701 ggctgcattt ttcctgagcg cggcggcgtt ttttccgcct caccgcacag cctttttcga
     2761 tgttataaaa aattgttcaa aaataaaaac gatcgatatt taataacatg aaaaaatgcc
     2821 gtgcggtaag gatgccgcac gcgtagacat gatccgtatc tcgcctgttt ggcaccgctg
     2881 cacgggcgat ggtatatcta caagcgggat ttgttagtct gaaatctcta ccggcaagga
     2941 ggcgcggatg cagcagctga gcgggctcga cgcggtattc ctcaacctcg agaccaacgc
     3001 ggtgccgatg catatcggca gcctgatcat cctcgatccg actaccgccg gccccggctt
     3061 cggttttgcc agggtgcgcc agctggtcgc cgagcgcctg cacctgctgc ccggcttccg
     3121 ccgccgcctg ctgtccacgc cgtggaatct ggaccagccg tactggctgg aagacccgga
     3181 cttcgacatc gagaaccacg tgcgccaccg cgccctgccc gcgccgggca acgcgcagca
     3241 gctgtcggaa ttcatctgcg acatcttcgc cacgcggctg gaccgcgagc gcccgctgtg
     3301 ggtgttctac tacatcgaag ggctggaagg cggcaaggtg gcgtgcctgt cgaagatcca
     3361 ccacgcctgc atcgacggcc tgtccggcgc cgaactgctg tccaacctgc tcgacaccac
     3421 gccgcagggc cgcgaggtgc cgccgccgga agcgccgtgg atgccggatg aaattcccac
     3481 gccgtggaag ctcacctata cgacggccaa gaacgtgctg tcgcgccccg gcgagatcta
     3541 ccggctggcg cgcgagacgc tgccgctgct gatgtcggcg cgcaaggaga tgctggccca
     3601 gcagcacgcg gcggaaaaat ccgccacgcc caccagcggc ggcgcgccgc gcatgcgctt
     3661 caacaccacc attacctcgc gccgctcgta cgccttccgc acggtggcgc tggccgacat
     3721 caagctgatc aagaacgcct tcggcgtatc ggtcaacgac gtggtcctca ccgtatgcgc
     3781 cgaagcgctg cgcaactatc tgctggaaaa aaaggaactg cccaggcagc cgctggtggc
     3841 ggccatcccg gtatcggtgc gcagcggtga cgagcgcggt gccggcggca accgcgtgac
     3901 catggcgcgc gcgccgctgg gcacgcatat cgccgacccg gtcgagcgcc tgctggccat
     3961 gagccgcaag atgggcgacg tgaagaagac catgaaggcg atccccgcgc ggctgatgct
     4021 cgactgggcc gacgcgccgc cgccggcggt gatggcgcag gccgcgcgcc tgtacgagaa
     4081 cttctcgatc cagaactaca tgagcccgcc gttcaacctg gtgatctcca acgtgccggg
     4141 accaccgggc gcgctgtact tcgccggcgc gcgggtcgaa gccaacttcc ccgtgtccat
     4201 tccctaccac gggctggcct gcaacatcac cgtgttcagc ctgcaggaca acctcgacat
     4261 cggcatcacc gcgcatcgcg ggacagtgcc ggacatcgac cacctgatgg acctgctggt
     4321 cgacgcggtg gcagtgctca agcaacgcgc gggcgcgcaa ctcaagaagg cgagctagaa
     4381 caacatggcc gatctcgatc gcttgatcca gaccctctac gcctcggcgc tggaaaccga
     4441 ccgcgagcag ttccgcccgc tggcgctgaa ggaagtgtgc cagtggctcg gcgccaccgg
     4501 cgccgcctgg ctgacgcgct cggtcaccgg actgcccggc gaatacaccg agtgcccgga
     4561 agcgggcatc tccatcgagc agctgtcgcg gctcaatttt tccgacggcg tgcgcgaata
     4621 cgtgctggac ccgctgccgc cggaactgtc gcccggcggc aaggccagcg gccaggaaca
     4681 gggctacctg atgcactacg tgcaccgcgg gggcgggctc aacagcaccg tgctgctgcg
     4741 cgcgccgcgc ggtcgcaagt tgcagcacca gggcgacctg cgccgcgcca tcggccacct
     4801 ggtggaagcc ggcacgctgt cgctgcgcca gtacatccac cgtgacgaaa aactctacga
     4861 gctcggccgc gccagccgcg gcaccgcggc gctggttgac gcgcagggca ccatctacgc
     4921 ggccagcacg cgtttccgcg aactgctgtc gggcgagttc ggcgaccaca atttcaccgc
     4981 gctgccgcag cccctgccgg aagaagcgca caccggcgaa ggcctgttct tccagggcgc
     5041 gctgcatttc cgctcgtcga agaagggccg cctgttcctg ctgcacgcgc gccggccgct
     5101 gccgctcgac ggcctgtcgc cgcgcgagca gcagatcgcg cgcgcgctgg gcacgggcaa
     5161 gaccttcaag agcgtggcgc gccagttcgg catcgccgtg tccaccgtgg ccaaccacgc
     5221 ctcgcgcatc taccgcaagc tcggcatctt ccgccgcgag gagctggtgg agatggtgcg
     5281 cacgcccacc agcaacgaca gcgaatgagc ggccgccggc cgcgcgccgg cggcatatcg
     5341 gcggcatcgg gcctgcgtgg ctgagacgat ggaattcgcc taccagctga tttttgccgc
     5401 gggcctgctg ttcctggtca gcatcgttgc caccacgctg acgccgcgcc tgggcgtgcc
     5461 actgctgctg gtattcatcg tcatcggcat gctggccggc gaggacgggc ccggcggcat
     5521 ccatttcgac gacctgcgcc tgaccaacct ggccagcagc gcggcgctgg cggtgatcct
     5581 gttcgacggc ggcatgcgca cgtcgatccg caccttccgc gtcggcctgt ggccggccat
     5641 ctcgctggcc acgatcggcg tggggctgac cacgctggtg gtcggctggt gcggccacaa
     5701 gctgctgggc ctgccgctgc cggtggcgct gctgctcggc gccatcgtca gctccaccga
     5761 tgccgccgcg gtgttctcgc tgctgcacta ccgctcggtg gcgctgaacg agcgcgtcac
     5821 ctcggtgctg gagatcgagt ccggcaccaa cgatccgatg gcggtgttcc tcacgctggc
     5881 gctgctgtcg tacatcgctg cgccggccga ttacggcgcc ggcgatttcc tcatgcagtt
     5941 cctcgcccag gccggcatcg gcggcgcgct cggcgcggcc ggtggctggc tgctggtgcg
     6001 cgcgctcaac aagctggaac tgtccgagcc gctctacccg gtcgcggcgc tattcagcgc
     6061 gctgctgctg ttcggcctca ccgcgctgct cgacggcagc ggattcctcg ccgtgtatct
     6121 cgccggcctc atcgtcggca accggcgcgt gcgcgccatg gcaagcatcc gccgcttcca
     6181 cgacgccatt gcgtggatgg cgcagatcgg catgttcctg atcctcggcc tgctggcggc
     6241 accgcaccgg ctggccgagg ttgcgctgcc cgcgctgctg ctcggcctgg tgctgatctt
     6301 cgccg
//
LOCUS       DASZZC010000752         9668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_696867,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000752 DASZZC010000000
VERSION     DASZZC010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9668
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696867"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2987)
                     /locus_tag="VHE37_16110"
     CDS             complement(<1..2987)
                     /locus_tag="VHE37_16110"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVT37114.1"
                     /translation="MTAPQAARQPTVQFYVAALALLLLLLEWASLSGVCPSPLQSLVH
                     TLPLVPASFTMDGGRFGLLAVLYLFAERRLIDRLAVLLGGVAIAATVYAANPQAGWGL
                     VQFAGNLFNGAGLAALIRTWAMRLDPVEDQRQYAVQLLWAYAVTLSLSLVIGPLLSAG
                     AELHPATYDFQLFRIDSALGLDLSARLGGLARLHPWLGNLLVIVYMLEPCALMALYAL
                     QIRRPLSGEIRALDLMVGSGLAAWLAYNLYPVCGPAYAFGTEYFPLKLPAPAAVPHGL
                     SVIAHDPRNGMPSMHFGWALAMWVIALRRGWPVRVAFSLVLALTAIATIALGEHYVLD
                     LLFCIPFLLALLAVLSFHLPMSGVRLQAIAFGGSAYLVCICLLVRWPDLLAQSPLGVW
                     SMVLCSVLPSVPLYWRLLRERPGDLPAAVSIDIQAEDRLLSRLPWVIGSCTSVAAMGQ
                     LLQLQQAFGDGSVMVPAALAGCIAGLAIGARNARRWRTLALKPMAVIAGCCLAAAIYA
                     VFFRYIGAGALRGYLAWARDTDADAPHAALVRAVLGAMVQLVPGICSGAAITMLAGLS
                     GNAPAAVGKRVLRAVLGLAIGIAVAGYLLLPVIGVRRTLLVAALVYMVLALLALNRSK
                     RRVMAAAAVDKPALNIHVWVGAALAGSMSAALLLILLKLAGLVAGANIYTSTGAWAVA
                     ATGLASGAALGMVAARGVDRRQALIGVAAVLVLLLLVVIAVAGAAPGYFSSFGLTQVA
                     PGFGEREIIRSLVAGLLVVPAALVIGGGWAVARAEDMSAALGGGAAGLLIAVAALQMI
                     GPLPLLQVACAIGFATFAVLVWRWHRVARWRFAAGAVVAGVLLLPRAATDAGLQAGSY
                     RDFRPQQQADAMPAADIYLRYTPARDAGLLLARGAGEAKFLSAAGFRTVDSVEAGTNR
                     ASVRGRLMLMSRQYDLIAAGDATSGDRAELSSREFYRLVAAHLRPRGIFAQPVALDRL
                     SPVELRYIVGSLRAEFAQVRVVVA"
     gene            3304..3732
                     /locus_tag="VHE37_16115"
     CDS             3304..3732
                     /locus_tag="VHE37_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HVT37115.1"
                     /translation="MKALQKGFTLIELMIVVAIIGILAAIAIPAYQDYIARGEIGSGL
                     ASVSPLKTAVEDLYARGATYTALADIGTSAGANALGTIDASVPTDGTAQLKFTWSNSS
                     PKTTGANIALDRDSSLGTWTCTYTPGAGDSGKKYLPKGCT"
     gene            3819..4268
                     /locus_tag="VHE37_16120"
     CDS             3819..4268
                     /locus_tag="VHE37_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HVT37116.1"
                     /translation="MRTHLHRGFTLIELMITVAIVGILAAIAIPAYQHYVARAHVASG
                     LATIYPLKTAVEDLLQNGHPAASITVTTVGANATANPLGTIEIPTFSADGTGNVKFRF
                     DRQSNPILKTPGAYLQLTRDGTTGTWSCQLIGLAPSETAKYNAKGCS"
     gene            4361..6091
                     /gene="pilB"
                     /locus_tag="VHE37_16125"
     CDS             4361..6091
                     /gene="pilB"
                     /locus_tag="VHE37_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HVT37117.1"
                     /translation="MAINPQVGNTPFLGGLARRLVADSLLGEEQAREHQQKALKSGTP
                     FVSLLVESKLVAPAKIAEAASNEFGVPLLDLGSVEIDQNAVKTVNEKIIRKTHALPIY
                     LRGKKMFVAVSDPTNLAALDEIKFATGTATEPILVEEDKLNRAIEAALTAIQSADMNV
                     GDADLEALDVEGGEAEAGPEITKSDAEDAPIVRFVNKIMIDAINIGASDIHFEPYEKT
                     YRVRYRKDGELKTIATPPVQQGGRLAARVKVMARLDLAERRVPQDGRIKLYLSKTKAI
                     DFRVSTCPTLFGEKICCRILDPSSAQLGIDALGYEPEQKEAYLKALAQPQGMILVTGP
                     TGSGKTVSLYTGLNILNTDDINISTAEDPAEINLPGINQVNVLPKVGLTFASALKAFL
                     RQDPDVIMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLVRLMNMGVPAYNLA
                     STLTLIIAQRLARRLCKNCKRPAKIPKQELLSEGFTEAEINDPGFSIMEAVGCDQCDG
                     GYKGRTGIYQVMPFSEEMGRIIMEGGNAMQIAEQATKEGVFDLRRSALKKVKDGAIDL
                     KQANACTVGD"
     gene            6116..7396
                     /locus_tag="VHE37_16130"
     CDS             6116..7396
                     /locus_tag="VHE37_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT37118.1"
                     /translation="MAEAAVARKPAPARKDKSKPVEVKEYTFKWEGTDKKGARVKGET
                     IGPSESMIKQQLRKMGINPLAVRKQMSLFGGSKKSGGKVTTGEVAIFARQMATMMTAG
                     VPLVQALEIVGRGHDNPAMGQMVLGIKTYIEGGASFSEALAKYPLQFDDLFVNLVDAG
                     EKSGALETLLDKIAVYKEKTEAIKKKVKKAMTYPTAVLVIAFVVSGILLYFVVPQFES
                     LFKGFGADLPAFTQWVINLSRWLQDYWWAVFIGIGGFFFTFFFFKKRSANFRRFLDRL
                     SLKIPIVGDILYKSAVARFARTLSTMFSAGVPLVEAMESVARASGNIVFEESILTMRD
                     QISTGQQLQLSMQQSGLFPNMAVQMIAIGEESGALDQMCAKVADFYEAEVDNMVDSLS
                     SLLEPMIMAVLGVVVGGLVIAMYLPIFKLGSVVG"
     gene            7436..8308
                     /locus_tag="VHE37_16135"
     CDS             7436..8308
                     /locus_tag="VHE37_16135"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HVT37119.1"
                     /translation="MALLEALRQDASWLLLTCTLLGLVVGSFLNVVILRLPRMLEQSW
                     RAEARQILELPAATDEPLVSLASPPSSCPGCGARIRPWHNIPLAGWLMLRGRCASCGM
                     PISIQYPVIEAISGLAAAICALHFGWSAQLLPAMVMSWTLIALTVIDLRTMLLPDNLT
                     LPLMWLGLLLSLFPVFASLHDSVVGAMAGYLSLRSMYHLFLLLTGKEGMGFGDFKLFA
                     AFGAWLGWQALPGIIIMSSVVGAVVGIGLIVFKRHGREVPIPFGPYLAGAGWIYMVWG
                     AQLRQAYFAASGIH"
     gene            8308..8913
                     /gene="coaE"
                     /locus_tag="VHE37_16140"
     CDS             8308..8913
                     /gene="coaE"
                     /locus_tag="VHE37_16140"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185261.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HVT37120.1"
                     /translation="MPRLVVGLTGGIASGKSLAADRFVALGVPVADADQASRDVVRPG
                     TPALTEIAREFGAEFITPAGELDRRRMREHVFSSPAARHALEAIVHPRIFEQLRLWRD
                     AQSAPYCMLSAAILLESGMKTLVQRVLVVDAEPAAQLQRLVGRDGIGEELGRAMISAQ
                     AARETRLAAADDVIRNDRDPAWLLQQVDRQHQRYLQLATEG"
     gene            8983..>9668
                     /gene="zapD"
                     /locus_tag="VHE37_16145"
     CDS             8983..>9668
                     /gene="zapD"
                     /locus_tag="VHE37_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HVT37121.1"
                     /translation="MTSGLDHVTFEQPLTERVRTFLRLEFLFAQHRHHQADHDGFGIR
                     ATLHALLDILTVLSRSDLKNEILKELTDRHVALTKLAARPGVDQERLQLILKDITASV
                     NGLQQLATQFAASVLRDNDFLTAVMNRSTIPGGTCAFDLPVYHYWLSQPYEIVERNLS
                     SWFADIKPFEQAILLYLRLLRSSVEPQEAVAQGGIYMQTPPGPCSLLRVHVPMDAHVY
                     PEISAGKHRFT"
BASE COUNT         1903 a         3177 c         3023 g         1565 t
ORIGIN      
        1 gccaccacca cgcgcacctg cgcgaactcc gcgcgcagcg atccgacgat gtagcgcagc
       61 tcgaccggcg acaggcgatc cagcgcgacc ggctgcgcaa atattccgcg cggccgcagg
      121 tgggcagcca ccagccggta gaactcgcgg ctgctcagtt cggcgcggtc gccggacgtc
      181 gcatcaccgg cggcaatgag atcgtattgc cgcgacatga gcatgagccg gccgcggacg
      241 ctcgcgcgat tcgttcctgc ctcgaccgaa tccaccgtgc ggaagccggc agcggacagg
      301 aatttcgcct cgcctgcacc gcgggccagg agcaacccgg catcccgcgc cggcgtgtag
      361 cgcaggtaga tgtccgctgc cggcatagcg tctgcctgct gctgcggtct gaagtcgcga
      421 tagctgccgg cctgcaaccc ggcatctgtc gccgcgcgcg gcagcagcag gacaccggcc
      481 accacggcac ctgcggcgaa tcgccagcgt gcaacgcggt gccagcgcca caccagcacg
      541 gcgaaggtgg cgaatccgat ggcgcatgcg acctgcagca gcggcagcgg cccgatcatc
      601 tgcaaggccg caactgcgat gagcaggccg gcagcaccgc cgcccagcgc tgcgctcatg
      661 tcctccgcgc gtgccaccgc ccagccgccg ccgataacca gcgccgccgg cacgaccagc
      721 aggccggcga caaggctgcg gatgatttcg cgctcgccga aacccggcgc cacctgcgtc
      781 agaccgaaac tggagaagta tcccggcgcc gcgccggcaa cggcgatgac cacgagcagc
      841 agcaacacga gcacggccgc aacaccaatc agcgcctggc ggcgatcgac cccacgtgcc
      901 gcaaccatgc ccagcgcggc acccgatgcc agccccgtgg ctgcgactgc ccacgcaccc
      961 gtcgaggtgt agatattggc cccggcgacg aggcccgcga gtttcagcag gatcagcagc
     1021 aaggccgcgc tcatgcttcc tgcaagtgcg gccccgaccc agacatggat attcaacgcg
     1081 ggcttgtcga ctgccgctgc agccatcacc ctccgtttgc tgcggttgag cgcgagcagt
     1141 gccagcacca tgtaaacgag tgccgcgacc agcaaggtcc ggcgcacgcc gatgaccggc
     1201 agcaacaggt agccggcaac agcgatgccg atggcgagtc ccaacacggc ccgcaacacc
     1261 cgcttgccga cagccgccgg ggcattaccc gagagcccgg ccagcatggt aatggccgca
     1321 ccggagcaga ttcccggcac cagctgcacc attgcgccca acacggcgcg cacaagtgca
     1381 gcgtgtggcg cgtccgcatc ggtatcccgc gcccaggcaa gatagccgcg aagcgcgccg
     1441 gcaccgatgt agcggaagaa aaccgcgtag atcgcagcag cgagacagca gccggcgatc
     1501 accgccatgg gcttgagcgc gagcgtacgc cagcgtcttg cgttccttgc gccgatggcg
     1561 aggccggcaa tgcagcccgc cagcgccgca ggcaccatga cacttccgtc gccgaaggcc
     1621 tgctggagct ggagcagctg tcccatcgcc gccaccgatg tgcatgagcc gatcacccat
     1681 ggcaatcgtg acagcagtcg gtcctcggct tggatgtcga tcgatacggc tgccggcaaa
     1741 tcccccggcc gttcacgcag caaacgccag tacaacggaa cactgggcag tacgctgcac
     1801 aacaccattg accagacccc caaaggcgat tgcgcgagca ggtcgggcca gcgcaccagc
     1861 aggcagatgc ataccaggta cgcagaccca ccaaaggcga tggcctgcag gcgcacgccc
     1921 gacattggca ggtggaagct gagtacggcc agcagtgcga gcagaaacgg gatgcagaac
     1981 agcaggtcga ggacgtagtg ttcgcccagg gcaatggtgg cgatggcggt cagcgcgagc
     2041 accaggctga aagcgacgcg caccggccaa ccccggcgca gtgcaatgac ccacatggcc
     2101 agcgcccagc caaaatgcat cgatggcatg ccgttgcggg gatcatgggc aatcacgctc
     2161 aggccgtgcg gaacggcggc gggcgccggc agtttcagcg ggaagtattc cgtgccgaaa
     2221 gcgtatgccg gcccgcagac cgggtagagg ttgtaggcca gccaggccgc aaggcccgat
     2281 ccgaccatga gatccagcgc gcggatttcc ccgctgagcg gccggcggat ctgcaacgca
     2341 tacagcgcca tcagcgcgca gggctccagc atgtagacaa tgaccagcag gttgcccagc
     2401 cacgggtgca gcctggcgag gccaccgagc cgcgcgctca gatcaagtcc gagggcgctg
     2461 tcgatgcgaa aaagctggaa atcgtaggtg gccggatgca gttctgctcc cgcgctcagc
     2521 aaaggaccga tcaccagtga aagactcaag gtgacggcat aagcccagag cagttggacc
     2581 gcgtattgcc gctgatcctc caccggatcg aggcgcatcg cccatgtgcg gatcaaggcc
     2641 gcgagacccg cgccgttgaa caggttgcct gcgaactgca ccaggcccca tccggcctgc
     2701 gggttggccg catataccgt cgccgcaatg gcgacgccgc cgagcagaac ggcaagcctg
     2761 tcgatcaggc gccgttccgc aaacagataa agcaccgcca ggaggccgaa gcggccgccg
     2821 tccatggtga agctcgcggg gaccaaaggc agcgtatgaa ccaggctttg caggggtgag
     2881 ggacagacgc cactgagcga ggcccattcg agcagcagga gcagcagcgc aagtgcggca
     2941 acatagaact gcacggtcgg ctgacgggca gcctgtgggg cggtcatgcg atgggaactg
     3001 gcaggagcag aggctgcgtc aacgcagcgg tagtggctgg ccgattttca tgttccgacg
     3061 acggagccga tctgccggac gctccgaaac cgcgcgattc tatacaagcg tcatggccgg
     3121 caacggtgcc gtcacggcca acattttttg tcactgaccg acaaaggatg tgatgctcct
     3181 gcggcttcag ggcctgcgca aaactcacaa tcaattgatc ctcataaaaa aaatatcttg
     3241 gcacaggcac tgcgttaaag atggagctgt tagggatatc cttttccaaa tttggagaca
     3301 atcatgaaag cgttgcaaaa gggcttcacc ttgatcgaac tgatgatcgt cgttgcgatc
     3361 atcggcattc tggccgccat cgccatcccg gcctaccagg actacatcgc ccgcggcgaa
     3421 atcggttccg gcctggccag cgtcagcccg ctgaagacgg ccgtggaaga cctgtatgcg
     3481 cgcggcgcga cctataccgc gctggccgac atcggcacct ccgcgggcgc caacgcgctg
     3541 ggcacgatcg acgcttccgt gcccaccgat ggtaccgcgc agctgaagtt cacctggtcg
     3601 aactccagcc cgaagaccac cggcgcgaac atcgccctgg accgcgactc ctcgctcggc
     3661 acctggacct gcacctacac cccgggcgct ggcgactcgg gcaagaagta tctgccgaag
     3721 ggctgcacct gatctgtagc tgatctgtta tcggggaaag ggcccgcggt gggatgaccc
     3781 cgcgggcctt tttaatttga cgacaagggg ggcaacacat gcggacgcac cttcatcgcg
     3841 gcttcaccct gatcgagttg atgatcacgg tggccatcgt gggcatactg gctgccattg
     3901 ccatccccgc ataccagcac tacgtggcac gcgcacacgt ggcgtccggc ctggcaacga
     3961 tttacccgct gaagaccgca gtcgaagacc tgctgcagaa cgggcacccg gccgcttcca
     4021 tcaccgtgac gacggtgggc gcgaatgcaa cggccaatcc gctgggcact atcgagattc
     4081 caaccttctc ggcggacggc accggcaacg tcaagttccg gtttgaccgc cagtcgaacc
     4141 ccatcctgaa aacgcccgga gcctacctgc agctgacccg cgacggcacc acgggcacct
     4201 ggtcgtgcca gctgatcggc ctggccccgt cagaaacggc gaaatacaac gccaagggct
     4261 gtagctgatc ccgctgcggc gatccggggt tgtcacgcaa catcctgcgc ccggtcaagt
     4321 tgtggtaaat agtacgccac ggatcgaggc gtaatttctc ttggctatca accctcaagt
     4381 cggcaacaca ccctttctcg gtggcctggc tcggcggctg gtcgctgact cgctgctcgg
     4441 cgaagagcag gcccgggaac atcagcagaa agcgctgaag tcagggacac cttttgtatc
     4501 gttgctggtc gaatccaagc tggtagctcc agccaagatt gcggaagcgg caagcaatga
     4561 attcggcgtt cccctgctcg acctcggctc ggtggaaatc gaccagaacg ccgtcaagac
     4621 ggtcaacgaa aaaatcatcc gcaagacgca cgcgctgccg atctatctgc gcggcaagaa
     4681 gatgttcgtg gcggtttccg acccgaccaa cctcgcggcg ctcgacgaga tcaagttcgc
     4741 cactggcacg gccaccgagc cgatcctggt ggaagaagac aagctgaacc gggccattga
     4801 agcagcgcta accgccatcc agtcggccga catgaacgtc ggcgatgccg accttgaagc
     4861 cctcgacgtc gagggcggcg aagcggaagc gggtccggaa atcaccaagt cggatgccga
     4921 agacgcgccg atcgtgcggt tcgtcaacaa gatcatgatc gacgccatca acatcggcgc
     4981 ttcggacatc cacttcgagc cttacgaaaa aacctaccgt gtccgctacc gcaaggatgg
     5041 cgagctcaag accattgcca cgccgcccgt gcagcagggc gggcgcctgg cggcgcgcgt
     5101 caaggtgatg gcgcgcctcg acctggcgga gcgccgcgtg ccgcaggacg gccgcatcaa
     5161 gctgtacctg tccaagacca aggcgatcga tttccgcgtc agcacctgcc cgactttgtt
     5221 cggtgagaag atctgctgcc gtattctgga tccgtcaagc gcgcagctgg gcatcgacgc
     5281 gttgggctac gagccggaac agaaagaggc ctatctcaag gcgctggccc agccgcaggg
     5341 catgatcctg gtgacgggac ccaccggctc gggcaagacc gtttcgctgt acaccggcct
     5401 gaacatcctc aacaccgacg acatcaacat ttccaccgcg gaagatccgg cggaaatcaa
     5461 cctgcccggc atcaaccagg tcaacgtgct gcccaaggtg ggcctgacct tcgcctcggc
     5521 cttgaaagca ttcctgcgcc aggatcctga cgtcatcatg gtcggcgaaa tccgtgacct
     5581 ggaaaccgcc gagatcgcga tcaaggcggc gcagacgggt cacctggttc tctccaccct
     5641 gcacaccaac gacgcaccgc agacgctcgt acgcctgatg aacatgggcg tgccggcgta
     5701 caacctggct tcgacgctga cgctgatcat cgcgcagcgc ctggcgcggc ggctgtgcaa
     5761 gaactgcaag cggccggcca agatacccaa gcaggaactg ctgtccgaag gcttcaccga
     5821 ggcggaaatc aacgatcccg gcttcagcat catggaagca gtgggctgcg atcagtgcga
     5881 tggcggctat aagggccgca ccggcatcta ccaggtcatg ccgttttccg aggagatggg
     5941 acgcatcatc atggaaggcg gcaacgccat gcagattgcc gagcaggcaa ccaaggaagg
     6001 cgtgttcgat ctgcgccgct ccgccttgaa gaaggtgaag gacggcgcga tcgatctcaa
     6061 gcaggccaat gcctgcacgg ttggcgactg attcgccgct tagctgagaa ccaacatggc
     6121 tgaagcagct gtcgcaagga aacccgcccc ggcgcgcaag gacaagtcca agccggtcga
     6181 ggtcaaggaa tacaccttca aatgggaagg tacggacaag aagggcgctc gcgtaaaagg
     6241 ggagaccatc ggccccagcg agagcatgat caagcagcag ctgcgcaaga tgggcatcaa
     6301 tccgcttgcc gtgcgcaagc agatgagcct gttcggcggc agcaagaaat ccggcggcaa
     6361 ggtcaccacc ggtgaagtcg ccatcttcgc ccgccagatg gcgacgatga tgaccgccgg
     6421 cgtcccgctg gtgcaggcgc tggagatcgt cggacgcggc cacgacaacc cggcaatggg
     6481 ccagatggtg ctcggcatca agacctacat cgagggcggc gcctcgttct ccgaggcgct
     6541 ggcaaagtac ccgctgcaat tcgacgacct gttcgtcaac ctggtcgacg ccggcgaaaa
     6601 gtccggtgca ctggaaacgc tgctcgacaa gatcgccgtc tacaaggaaa agaccgaggc
     6661 gatcaagaag aaggtcaaga aggcgatgac ctacccgacc gcggtgctgg tcattgcctt
     6721 cgtcgtgtcc ggcatcctgt tgtactttgt ggtgccccag ttcgaatccc tgttcaaggg
     6781 gttcggcgcg gacctgccgg ccttcaccca gtgggtgatc aacctgtcgc gctggctgca
     6841 ggactactgg tgggcggtgt tcatcggcat cggcggattc ttcttcacct tcttcttctt
     6901 caagaagcgc tccgcgaact tccgccgttt cctcgaccgc ctctcgctga agatcccgat
     6961 cgtgggtgac atcctgtaca agtcggcggt ggcacgtttc gcccgtacgc tgtcgaccat
     7021 gttctcggcc ggtgtgccgc tggtggaagc catggaatcg gtggcgcgcg catccggcaa
     7081 catcgtgttc gaggaatcga tcctcacgat gcgcgaccag atctccaccg gccagcaact
     7141 gcagctgtcg atgcagcagt ccgggctgtt ccccaacatg gcggtgcaga tgatcgccat
     7201 cggcgaggaa tcgggtgccc tggaccagat gtgcgccaag gtggccgact tttacgaagc
     7261 agaagtcgac aacatggtcg actcgctgtc tagcctgctc gagcccatga tcatggccgt
     7321 cctcggcgta gtggtcggcg gcctggtcat cgcgatgtac ctgccgatct tcaagctggg
     7381 atcggtggtt ggctgaccgc ggcaatcatc acgcttaaaa accgggggtt catgcgtggc
     7441 tctgcttgaa gcgctgcgac aagacgcgtc gtggctgttg ctgacctgca ccttgctggg
     7501 cctcgtggtc ggcagcttcc tcaacgtggt gatcctgcgc ctgccgcgca tgctggaaca
     7561 gtcctggcgc gccgaggcgc gccagatcct ggaactgccc gccgccaccg acgagccgct
     7621 ggtctcgctg gcctcgccgc catcgtcctg ccccggctgc ggtgcacgca tccgcccctg
     7681 gcacaacatt ccgctggccg gctggctgat gctgcgcgga cgctgcgcca gctgcggcat
     7741 gcccatctcc atccagtatc cggtcatcga agccatcagc gggctggcgg cggcgatctg
     7801 cgcactgcat ttcggctgga gcgcgcagct gttgccggcg atggtgatga gctggacgct
     7861 gatcgcactg acggtcatcg atctgcgcac catgctgctg cccgacaatc tcacgctgcc
     7921 gctgatgtgg ctgggattgc tgctcagcct gttcccggta ttcgcgagcc tgcacgacag
     7981 cgtggtcggc gccatggcgg gctacctgag cctgcgctcg atgtatcacc tgttcctgtt
     8041 gctcaccggc aaggagggca tgggcttcgg cgacttcaag ctgtttgccg cattcggcgc
     8101 atggctcggc tggcaggccc tgcccggcat catcatcatg tcgtccgtgg ttggagccgt
     8161 ggtcggcatc ggcctgatcg tgttcaagcg ccacggccgc gaagtgccaa ttcctttcgg
     8221 cccctacctc gcaggtgcag gctggatcta catggtctgg ggtgcgcagt tgcgccaggc
     8281 ctacttcgcc gccagcggca tccactgatg ccgcggctgg tggtgggcct caccggcggc
     8341 atcgccagcg gcaagtcgct cgcagcggac cgcttcgtgg cgctgggggt gccggtggcg
     8401 gatgccgacc aggcatctcg ggacgtggtg cggcccggta ccccggcgct caccgaaatc
     8461 gccagggaat tcggcgcaga gttcatcaca cccgcgggcg agctcgaccg ccgccgcatg
     8521 cgcgagcatg tgttttcttc tcctgctgcg cgccatgcgc tggaagccat cgtccatccg
     8581 cgcatcttcg agcaactcag gctctggcgt gacgcgcaat cggcgcccta ttgcatgctc
     8641 agcgcggcca tcctgctgga gagcggcatg aagacactgg tgcagcgtgt gctggtggtc
     8701 gacgccgaac cggcagccca gttgcagcgg ctggtggggc gcgacggcat cggcgaagaa
     8761 ctgggacgcg ccatgatttc ggcccaggcc gcgcgcgaaa cgcggctggc tgcggccgat
     8821 gacgtaatcc gcaatgatcg cgatcccgcc tggctcctgc agcaggtgga ccggcagcac
     8881 cagcgctacc tgcagctggc caccgagggt tgaaacgcga cgatttcgtt gaattaggtt
     8941 gagactacgg tcacaatcgc ggcgtttgtc acagcctgcg ccatgacctc agggttggat
     9001 cacgtcacct tcgagcagcc gttgaccgaa cgggtccgga ccttcctgcg cctggaattc
     9061 ctgttcgccc agcaccggca ccaccaggcc gaccacgacg gcttcggcat ccgcgccacg
     9121 ctccacgccc tgctcgatat cctcacggtg ctgtcgcgct cggacctgaa gaacgagatt
     9181 ctcaaggaac tgaccgatcg tcatgtcgcg ctgaccaagc tggccgcccg acccggcgtc
     9241 gaccaggaac ggctgcagct catcctcaag gacatcactg cgtcggtgaa tggactgcag
     9301 cagctggcca cgcagttcgc cgcgtcggtg ctgcgcgaca acgattttct caccgcggtg
     9361 atgaaccgct cgaccatccc cggcggcacc tgcgcgtttg atctgccggt gtatcactac
     9421 tggctgtcgc agccgtacga gatcgtggag cgcaacctgt catcgtggtt cgccgacatc
     9481 aagccgttcg agcaggcgat cctgctgtac ctgcgcctgc tgcgcagcag cgtcgagcca
     9541 caggaagcgg tggcgcaggg cggcatctac atgcagacgc cgcccggccc gtgctcgctg
     9601 ttgcgcgtgc acgtaccgat ggacgcgcac gtttacccgg aaatcagtgc cggcaagcat
     9661 cgcttcac
//
LOCUS       DASZZC010000753         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_697115,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000753 DASZZC010000000
VERSION     DASZZC010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697115"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1655
                     /locus_tag="VHE37_16150"
     CDS             <1..1655
                     /locus_tag="VHE37_16150"
                     /inference="COORDINATES: protein motif:HMM:NF039312.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT37122.1"
                     /translation="QLDGELNGELARLRLDSQLDARGLAALAQKLGAARDALVQGESA
                     LDSLPPAVDKPYSAALGELKDKLAQVQDYLARREEFVKADRQLHEALVQAHGSKAQAQ
                     LDALLDNVAAYLREPTPETGSRLVNLIAQIKAGAGEVADAVRRVQLQQVADGAVAVLS
                     ARDQEQAGAAAVLALPTRSHLEQFKAAYTEYTRSELAATKRYRQVMVIYTVALLGAFG
                     LIGFRLRQSMGALDRAYLKLQHTNANLEQTVEERTQDLRKALEDVRRQQAQLIQSEKM
                     ASLGQMVAGVAHEINTPLGYARSNVSIMRDSLAPLEELLGGYELALAYLQDPQADAQE
                     RERRIKDLEHLRAAWNPTDDLPALAALLADADHGLGQIAELVLGLKDFSRVDRSRTEL
                     FNVNDGLDTALKICHNQLKERIEIVREYGELPEIVCAPSQLNQVFLNIITNAGQAISG
                     GGQIRIRTRLDGDMVEIGIRDSGSGMDAATQARIFEPFFTTKAVGQGTGLGLSIVYSI
                     IQDHKGSIAVQSAPGQGTEFVIRLPRGAAAPKKIPAVEMAAA"
     gene            complement(1659..>2117)
                     /locus_tag="VHE37_16155"
     CDS             complement(1659..>2117)
                     /locus_tag="VHE37_16155"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT37123.1"
                     /translation="KADAERKHQAEQQAAAAAAQAAAAQAAEAQRKEQERQAAARAAS
                     TVASAAPAAAKEHGPASAAKVDWTSCSQPSYPSASLRRREEGTVMMAFHIDPSGHVTG
                     QRIDKSSGSPRLDQAAVDAIVKCRFMPAQADGEAVDSWASLRFVWKLDGG"
BASE COUNT          349 a          719 c          748 g          301 t
ORIGIN      
        1 cgcagctcga cggcgaactc aacggtgaac tcgcgcggct gcggctcgac agccagctgg
       61 acgcacgcgg cctcgccgcg ctggcgcaga agctgggcgc ggcgcgcgac gcgctggtgc
      121 agggcgaaag cgccctggac agcctgccgc cggcggtcga caagccctac tcggcggcgc
      181 tgggtgaact caaggacaag ctggcccagg tgcaggacta cctcgcgcgg cgcgaggaat
      241 tcgtcaaggc cgatcgccag ctgcacgagg cgttggtgca ggcgcacggc agcaaggcgc
      301 aggctcagct cgatgcgctg ctcgacaacg tggcggcgta tctgcgtgag cccacgccgg
      361 aaaccggcag ccggctggtg aacctgatcg cgcagatcaa ggccggcgcc ggcgaagttg
      421 ccgatgccgt gcgccgggtg cagctgcaac aggtggccga cggcgcggtg gcggtgctgt
      481 cggcgcgcga ccaggaacag gccggtgcgg cggcggtgct ggcactgccc acgcgcagcc
      541 atctggagca gttcaaggcg gcctacaccg agtacacgcg cagcgagctg gccgccacca
      601 agcgttatcg ccaggtgatg gtgatctaca cggtggcgct gctcggtgcg ttcggcctga
      661 tcggcttccg cctgcgccag agcatgggcg cgctggaccg cgcttacctc aagctgcagc
      721 acaccaatgc caacctcgag cagaccgtcg aggagcgcac gcaagacctg cgcaaggcgc
      781 tggaagacgt gcgccgccag caggcgcagc tgatccagtc ggagaagatg gcgtcgctcg
      841 gccagatggt ggccggcgtg gcccacgaga tcaacacgcc gctgggctat gcgcgcagca
      901 acgtgtcgat catgcgcgat tcgctggcgc cgctggagga actgctcggc ggctacgaac
      961 tggcgctggc ctacctgcag gatccgcagg ccgatgcgca ggagcgcgag cggcgcatca
     1021 aggatctcga acacctgcgc gccgcatgga accccaccga tgacctgccc gcgctggccg
     1081 cactgctggc cgatgccgac cacgggctcg ggcagatcgc cgaactggtg ctggggctga
     1141 aggatttctc gcgcgtggac cgctcgcgca ccgaactgtt caacgtcaac gacgggctcg
     1201 ataccgcact gaagatctgc cacaaccagc tcaaggagcg catcgagatc gtgcgcgagt
     1261 acggcgagct gccggaaatc gtctgtgccc cgtcgcagct caaccaggtg ttcctcaaca
     1321 tcatcaccaa cgccggacag gcgatcagcg gcggcggcca gatccgcatc cgcacgcggc
     1381 tcgacggcga catggtggag atcggcatca gggacagcgg cagcggcatg gacgcggcga
     1441 cgcaggcacg catcttcgag ccgttcttca ccaccaaggc ggtgggccag ggcaccgggc
     1501 tggggctgtc gatcgtctac agcatcatcc aggaccacaa gggcagcatc gcggtgcagt
     1561 ccgcaccagg gcagggcacc gagttcgtca tccgcctgcc gcggggcgca gcggccccga
     1621 agaagatacc cgcggtggag atggcggcgg cctgaacctc agccgccgtc gagcttccag
     1681 acgaagcgca gcgaggccca gctgtccact gcctcgccgt cagcctgggc cggcatgaag
     1741 cggcatttga caatggcgtc gaccgccgcc tggtccagcc gcggcgagcc actgctcttg
     1801 tcgatgcgct ggccggtgac atgcccgctg gggtcgatgt ggaacgccat catcaccgtg
     1861 ccttcctcgc gccggcgcaa tgaagcggac gggtaactcg gctggctgca cgaggtccag
     1921 tccaccttgg cggcgctggc cggcccgtgt tcctttgctg ccgcgggtgc cgcgctggcc
     1981 acggtgctgg cggcgcgagc ggcggcctgc cgctcctgtt ccttgcgctg cgcctcggct
     2041 gcctgggcgg cagcagcctg tgcggctgcg gccgccgcct gctgctcggc ctggtgcttg
     2101 cgttcggcgt cggcctt
//
LOCUS       DASZZC010000754         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1279836,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000754 DASZZC010000000
VERSION     DASZZC010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279836"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="nirD"
                     /locus_tag="VHE37_16160"
     CDS             complement(<1..1117)
                     /gene="nirD"
                     /locus_tag="VHE37_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HVT37124.1"
                     /translation="MMGAMELPGDGVRVCAVDDILPDMGVCALVGGRQVAVFRIGETD
                     EVHAIGAYDPNSGANVLARGIVGDLRGRLVVASPIYKQHFDLHTGECIEDPVASVRRY
                     ETLVRDGQVYVGLPSSAKTAARRQRLAVIGNGMAAMRVVEELLRIAPELYDIAVFGSE
                     AAGNYNRILLSPLLAGDKQLDEIMLNTPEWYARRGVVLHADDPVVRIDRRRCTLRTRS
                     GYEMRYDRVLLATGSNPVVTPVPGNKLPGVVTFRDLRDVEQMTETAKNHRQAVVIGGG
                     LLGLEAANGLLRRGMSVSVVHLLDSLMERQLDRHAGAMLKTSLEARGLKFIMPAQTEE
                     IIGSDRATGVRFKDGREIPADLVVMAVGIRPNIELAQS"
     gene            complement(1114..>3637)
                     /gene="nirB"
                     /locus_tag="VHE37_16165"
     CDS             complement(1114..>3637)
                     /gene="nirB"
                     /locus_tag="VHE37_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HVT37125.1"
                     /translation="IGHGMVGHKFLEQLARGSAPVQVSVLCEEPRPAYDRVHLTDYFG
                     GKSAADLSLVQEGFFEGAGYALHVDDKAVAIDRASRLVRSAGGRELAYDKLILATGSY
                     PFVPPLPGKERPGCFVYRTIDDLDAISAAAKTGKVGVVIGGGLLGLEAAKALKDLGLE
                     VNVVEFAPRLMAVQVDDGGGRVLRQKIEELGVRVHTGKNTQAIEDGESCRHRMKFADG
                     SVLETDVIVFSAGIRPRDELARSAGIEVGERGGIRINEFCQTSDPDIYAIGECALYAG
                     KIYGLVAPGYQMAMTAAAHLCGQAETGFSGADMSTKLKLLGVDVASVGDAHAATPGAR
                     TYAYSDEVKRIYKKLVVSSDHKLLLGAVLVGEAEEYGTLLQMMLNGIPLPTHPEDLIL
                     PRRDAGGEKGGGLGMAALPAAAQICSCNNVSKGAICGAIDSGCTTLGDLKKKTKAATS
                     CGGCGPLVTQILKHELASRGVAVSNALCEHFAHTRQELYHLVRVDQIKDFDTLIARHG
                     QGRGCDICKPTAASIFASCWNEHVLKPMHHKLQDSNDYYMANIQKDGTYSIVPRVPGG
                     EITPDKLIVLGAVAKKYGLYTKITGGQRIDLFGAQVHELPLIWKELVDAGFESGHAYG
                     KALRTVKSCVGSTWCRYGVQDSVKLAIDLEERYRGLRSPHKIKLAVSGCTRECAEAQG
                     KDVGVIATEKGWNLYVAGNGGMKPRHADLLARDLDTGTLIKYIDRFLMFYVRTADRLQ
                     RTSVWLENLEGGLDYLREVVCEDKLGIAASLESEMAHVIATYECEWKKAINDPQTLKR
                     FRHFVNSDATDDNVLFVQERGQIRPANDAERQRARAAKLESA"
BASE COUNT          553 a         1249 c         1172 g          663 t
ORIGIN      
        1 aggattgcgc caactcgatg ttcggccgga tgccgaccgc catcaccacc aggtcggcgg
       61 gaatttcgcg gccgtccttg aagcgaacgc ctgtggcccg gtccgaaccg atgatctctt
      121 cagtctgcgc cggcatgatg aatttcaagc cgcgcgcttc gagcgaggtt ttcagcatgg
      181 cgccggcgtg cctgtcgagc tggcgctcca tcagcgaatc gagcaggtgc accacgctga
      241 ccgacatgcc gcggcggagc aggccgttgg cggcttcgag gccaagtagt ccaccgccga
      301 tcaccaccgc ctgcctgtgg ttcttcgccg tttccgtcat ctgctccacg tcgcgcaggt
      361 cgcggaaggt gaccacgccg ggcaacttgt ttccgggaac gggagtcacc accgggttgg
      421 agccggtggc cagcagcacg cggtcatagc gcatctcgta gccgctgcgc gtgcgcagcg
      481 tgcagcggcg ccggtcgatg cgcaccaccg ggtcatcggc atgcagcacc acgccgcggc
      541 gcgcgtacca ctccggcgtg ttgagcatga tctcgtccag ctgcttgtcg ccggccagca
      601 gcggcgacag caggatgcgg ttgtagttgc cggccgcctc cgagccgaac accgcgatgt
      661 cgtacagctc gggcgcgatg cgcagcagtt cctccaccac gcgcatcgcc gccatgccgt
      721 tgccgatcac cgccaggcgc tggcgccgcg cggcggtttt cgccgacgac ggcaggccta
      781 catagacctg gccgtcacgc accagcgtct cataccggcg caccgaggcg accgggtcct
      841 cgatgcattc gccggtatgc aggtcgaaat gctgcttgta gatcggcgac gccaccacca
      901 gccgccctct cagatcgccg acgatgccgc gcgccagcac attggcgccg ctgttgggat
      961 cgtacgcgcc gatggcatgc acctcgtcgg tttcgccgat gcggaacacc gccacctgcc
     1021 tgccgccgac cagcgcgcac acgcccatgt cgggcaggat gtcgtcgacg gcgcagacgc
     1081 gcacaccgtc acccggcaat tccatcgcgc ccatcatgcg ctctcgagtt tcgccgcgcg
     1141 tgcgcgctgg cgctcggcgt cgttggcggg gcggatctgc ccgcgctcct gcacgaacag
     1201 cacgttgtcg tcggtggcat cgctgttgac gaagtggcgg aagcgcttga gcgtctgcgg
     1261 gtcgttgatc gccttcttcc actcgcactc gtaggtggcg atgacgtgcg ccatctcgga
     1321 ctccagcgag gcggcgatgc cgagcttgtc ctcgcacacc acctcgcgca ggtagtcgag
     1381 cccgccttcc aggttctcca gccacaccga ggtgcgctgc aggcggtcgg cggtgcgtac
     1441 gtagaacatc aggaagcgat cgatgtactt gatgagtgtc ccggtatcga gatcgcgcgc
     1501 cagcaggtcg gcgtggcgcg gcttcatgcc gccattgccg gcgacataga gattccatcc
     1561 cttctccgtg gcgatcacgc cgacgtcctt gccctgtgct tcggcgcact cgcgtgtgca
     1621 gccggacacg gccagcttga tcttgtgcgg cgagcgcagg ccgcggtagc gctcctccag
     1681 gtcgatggcg agcttgaccg aatcctgcac gccgtaacgg caccaggtgc tgcccacgca
     1741 ggacttcacc gtgcgcagcg ccttgccgta ggcatggccg gattcgaagc cggcatccac
     1801 cagttccttc cagatcagcg gcagttcatg cacctgcgcg ccgaacaggt cgatgcgctg
     1861 gccgccggtg atcttggtgt acaggccata tttcttcgcc accgcgccga gcacgatcag
     1921 cttgtccggc gtgatctcgc cgcccggcac gcgcggcacg atcgagtagg tgccatcctt
     1981 ctggatgttc gccatgtagt aatcattgct gtcctgcagc ttgtgatgca tgggcttgag
     2041 cacatgctcg ttccagcagg aggcgaagat gctggccgcc gtcggcttgc agatgtcgca
     2101 gccgcggccc tggccgtggc gtgcgatcag cgtgtcgaaa tccttgatct gatcgacgcg
     2161 gaccaggtga tagagctcct ggcgcgtgtg cgcgaagtgc tcgcacagcg cgttgctgac
     2221 ggcgacgccg cgactggcga gttcgtgttt caggatctgc gtgaccagcg gcccgcagcc
     2281 gccgcaggaa gtggcggcct tggtcttctt cttgagatcg cccagcgtgg tgcagccgct
     2341 gtcgatggcg ccgcagatgg cgcccttgct cacgttgttg cacgagcaga tctgcgccgc
     2401 tgccggcagc gcggccatgc cgaggccgcc acccttctcg ccgccggcgt cgcgccgggg
     2461 caggatcagg tcttccgggt gcgtgggcag cgggatgccg ttgagcatca tctgcagcag
     2521 cgtgccgtat tcctcggctt cgcccaccag caccgcgccg agcagcagct tgtggtcgga
     2581 cgacacgacc agcttcttgt agatgcgctt cacctcatcg gagtacgcat aggtgcgcgc
     2641 gccgggggtg gccgcgtgtg cgtcgcccac gctggccaca tcgacgccca ataatttgag
     2701 tttcgtactc atgtcggcgc cgctgaagcc ggtttcggcc tggccgcaca agtgcgccgc
     2761 cgcggtcatc gccatctggt agccgggcgc taccaggccg tagatcttgc ccgcgtaaag
     2821 cgcgcattcg ccgatggcgt agatgtcggg gtccgaggtc tggcagaact cgtttatgcg
     2881 gatgccgccg cgctcgccca cctcgatgcc tgcgctgcgc gcgagctcgt cgcgcgggcg
     2941 gatgccggcc gagaacacga tgacatcggt ctcgagcacg ctgccgtcgg cgaatttcat
     3001 ccggtgcctg cagctttcac cgtcttcgat ggcctgcgtg ttcttgccgg tgtgcacgcg
     3061 cacgccgagt tcctcgatct tctgccgcag cacgcggccg ccgccgtcat cgacctgcac
     3121 cgccatcaaa cgcggcgcga actcgacgac attcacttcc aggccgagat ccttgagcgc
     3181 tttggccgcc tcgagcccca gcaagcctcc gccgatcacc acgccgacct tgcccgtctt
     3241 cgctgccgcc gagatcgcat cgagatcgtc gatggtgcga tagacgaagc agcccggccg
     3301 ctccttgccc ggcagcggcg gcacgaaagg ataggagccg gtggccagga tcagcttgtc
     3361 gtaggccagt tcgcgcccgc cggccgagcg taccagtcgg ctggcccgat cgatggccac
     3421 tgccttgtcg tccacatgca gcgcgtagcc ggcgccttcg aaaaatcctt cctgcaccag
     3481 cgagagatcg gctgcgctct tgccgccgaa gtagtcggtg agatgcacgc ggtcgtaggc
     3541 cgggcgcggt tcttcgcaca gcacactgac ctgtaccggc gcgctgccgc gcgccagctg
     3601 ctcgaggaac ttgtggccca ccatgccgtg gccgatg
//
LOCUS       DASZZC010000755         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1280105,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000755 DASZZC010000000
VERSION     DASZZC010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280105"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..333)
                     /gene="rpsO"
                     /locus_tag="VHE37_16170"
     CDS             complement(64..333)
                     /gene="rpsO"
                     /locus_tag="VHE37_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HVT37126.1"
                     /translation="MTLSVEHKAGVVKKYARAKNDTGSPEVQVALLTERINELGGHFE
                     KHDKDSHSRRGLLKMVNQRRRLLDYLKASDEGRYNKLIADLGLRR"
     gene            complement(434..1372)
                     /gene="truB"
                     /locus_tag="VHE37_16175"
     CDS             complement(434..1372)
                     /gene="truB"
                     /locus_tag="VHE37_16175"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HVT37127.1"
                     /translation="MELISSKGTRQQKIARPRRAVNGILLLDKPLNLSSNQALQQAKR
                     LYQADKAGHTGSLDPLATGMLPICFGRATKLSHYLLDSHKRYLAVIRFGERTETGDAE
                     GAVVAYSDASTLTRVRLSESLQKFVGVIEQVPPMYSALKREGRPLYELARAGIEVERA
                     ARRVNIDELKVVSFEAGLCELEVACSKGTYIRTLAEDIAAACGQVAHLAALRRLYVRP
                     YEGLPMQTLDQIQAAASRGLESLDALLLSPATALRGWPELRLDQEAAARLRHGQAVAV
                     GRAPPGTALAVGGDGQLLAIVHVESGMAAPRRWLAD"
     gene            complement(1522..1998)
                     /locus_tag="VHE37_16180"
     CDS             complement(1522..1998)
                     /locus_tag="VHE37_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37128.1"
                     /translation="MRAVLLLPLLLAAAGNGCSRTYNDPVVARVDGSLITREQLDQEE
                     RRVAPGAALDPATRRKLLQSLVLDRALASAQERMLTADEMRVVEADQRSHRDQVLARM
                     YLAHHGAALAPSDDEVRTYYQQFCARRHAHRDHWLKFAPASSRPWPHNSCAVRWMR"
     gene            complement(1998..2825)
                     /locus_tag="VHE37_16185"
     CDS             complement(1998..2825)
                     /locus_tag="VHE37_16185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT37129.1"
                     /translation="MKKKNWFLAVACAGLLVACGGDKLATVNGEAVTREEFQAYLKYK
                     HLPVTDKEAARWQGALDQYLQRKGLAMAVEQAMKHSDVVDSALLQAEIDDFRREALVS
                     RYFEQYLSKQVTDDAVTSYYNQHAQDYSERRVHIAHILFRTTRAMSENERRAQLTKAQ
                     EAASKIRAGTDFAKVAEQVSEDAISAKKGGDLGWVREGAIDPKLSQIAFELKEGTVSE
                     PFETQFGFHIIKVLEAPQTKQRPLEAVKGDIRYRLRSEAKEAEVKRLTESVSIKKKG"
     gene            complement(3149..3727)
                     /locus_tag="VHE37_16190"
     CDS             complement(3149..3727)
                     /locus_tag="VHE37_16190"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVT37130.1"
                     /translation="MKRWLLALLLPAAAMAADRYELDPTHTYPEFSVSHMGLSLMHGR
                     FNETAGSFVLDRKGEGSSVQVTVKVASLDSGMPARDERLRADNFLDAAHFPEMSYRST
                     RVIFSGDKITRIEGQLTLHGVTRPLALEVTAMNCAIHAIKRVWACGFEAQGMLKRSDY
                     GITTYLPEVGDEVIIRVEAEGAREEKSVGVRH"
     gene            complement(3724..4170)
                     /locus_tag="VHE37_16195"
     CDS             complement(3724..4170)
                     /locus_tag="VHE37_16195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT37131.1"
                     /translation="MNAPVTREDFSHFIRIDTRWGDLDTLGHVNNAVFFTFDESARLD
                     YFGQMFGNDPKFWKDYGIILASIGCDFIAQLHHPSTLDIGLRIARIGRSSMTSQAGMF
                     VGNKLVAVSRGTIVWFSYLQQKSLPVPENVRAMIRAREKIKPEEAA"
     gene            complement(4167..4373)
                     /locus_tag="VHE37_16200"
     CDS             complement(4167..4373)
                     /locus_tag="VHE37_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HVT37132.1"
                     /translation="MIESPCIKVCQLDAQQVCVGCGRSLDEIAQWSTASEVRQRQIVE
                     VARQRLAIIARRPAGTSPDSSEPA"
     gene            complement(4449..>5213)
                     /locus_tag="VHE37_16205"
     CDS             complement(4449..>5213)
                     /locus_tag="VHE37_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT37133.1"
                     /translation="KPFVLAGLDAVHEDVLGESHWHFGIDAGGGLLVPTSFHGLALRA
                     EGRLQYQFNDQSVASESSLLDWRVGVGIQVPLYFLADKMGKTAAAPECGVAVVSPEGA
                     RPDCGVDSDHDGVPDAADQCPGTTAGTPVDAHGCPAGEAALMRNIQFRLDSAELTEDS
                     KKILDGVAATLKQPESANINVEIGGHTDTTGSDPYNQMLSQQRAESVKQYLIGKGVTA
                     ERLKTRGYGKAEPVADNTTEQGREENRRVAFRILVD"
BASE COUNT          916 a         1692 c         1546 g         1059 t
ORIGIN      
        1 ctctggttaa tcgagggtcg tcacgggcat gactgcccga cgctaaaacg gaaaacgccg
       61 ctgttagcgg cgcagtccga ggtcggcgat gagcttgttg tagcggcctt catcgctggc
      121 cttgagatag tcgagcaggc ggcggcgctg gttcaccatc ttgagcaagc cgcggcggga
      181 atgactgtcc ttgtcgtgct tctcaaagtg gccaccgagc tcgttgatgc gttcggtcag
      241 cagggccacc tgcacttccg gcgagccggt gtcgttcttc gcgcgtgcgt actttttgac
      301 cacgccggct ttgtgttcaa cagataacgt catgagggta aagctccagc tttcagaatt
      361 tgtcgatatt gagccagcta ttttagcttt ttcccttgcg ccctcacaag atagtttttg
      421 cccctttttt gcctcaatcg gcgagccacc ggcgcggggc agccatgccc gactcaacat
      481 ggacgatcgc caggagctgc ccatcccctc cgactgccaa ggctgttcct ggaggagccc
      541 tcccaaccgc gactgcctgg ccatgtcgca gccgggcagc cgcttcctga tctagacgca
      601 gttcaggcca gccgcgtaaa gccgttgccg ggctgagcag gagtgcgtcg agcgactcca
      661 agccgcgaga ggcggccgct tgaatctggt caagcgtctg catcggtaag ccctcgtacg
      721 ggcgcacata caggcgccgc aatgccgcca gatgcgccac ctgtccgcac gctgcggcga
      781 tgtcctcggc cagggttcgg atataagttc cctttgagca tgcgacttcc agctcgcata
      841 gcccagcttc gaaggaaacc accttcagtt cgtcgatgtt cacgcggcga gcagctcgct
      901 cgacctcaat gcccgcgcgc gccaattcat atagcgggcg gccttcgcgc ttgagggcgg
      961 agtacatggg cgggacctgt tcaatcactc cgacaaattt ctgcaggctc tcgctcaatc
     1021 gaacacgcgt cagtgttgat gcgtccgaat aggcaacaac agctccttcc gcatcgcccg
     1081 tttcagtccg ttcaccaaag cgaatcaccg ccagatatcg cttgtgcgaa tccaataaat
     1141 aatggctcag cttggtggca cgcccgaagc atattggcag catccctgtc gccagcggat
     1201 cgaggcttcc tgtatgcccg gctttgtctg cctgatagag gcgcttggcc tgctgcagcg
     1261 cctggttgga cgaaagattc aacggcttgt cgagcaagag aatgccattt acagcgcggc
     1321 gcggcctagc gatcttttgc tgcctagttc ccttgctgga aatcaattcc acggtgctct
     1381 gaaatgaaat tatggttttg caagtcacgc caagtccggc atctcaaatc gaccgcggtg
     1441 aatccggcga tagagcttca acatttcacg gctactctac tccgacatac tcaacgtttg
     1501 ccgccttgag caccgctggc gctacctcat ccagcgtacg gcgcaactgt tgtgcggcca
     1561 aggcctgctc gatgctggag cgaacttcag ccaatggtcg cggtgtgcgt gccgtctcgc
     1621 gcaaaactgc tggtagtacg tgcgcacctc gtcatcgctg ggagccaagg ccgcgccatg
     1681 atgagcaaga tacatgcgcg ccagtacctg atcgcggtgc gagcgctggt cggcctcgac
     1741 cacacgcatc tcatcggctg tgagcattcg ttcctgtgca ctcgccagag cccggtccag
     1801 caccaaactt tgcaggagct tgcgccgagt ggcagggtcc agcgctgcgc ccggcgctac
     1861 acgccgctct tcttggtcta gctgttcgcg cgtgatcagg ctgccatcca cgcgcgccac
     1921 caccggatcg ttgtacgtgc gagagcaccc gttcccagcg gcagccagca acagtggcaa
     1981 gagcagcacg gctcgcatca gcctttcttc ttaatgctga cgctctcagt gagccgcttg
     2041 acttctgctt ccttggcctc gctgcgaagt cggtagcgga tgtcgccctt cactgcctcg
     2101 agcggacgct gcttggtctg cggcgcttcc aacaccttga taatgtggaa gccgaattgc
     2161 gtctcgaacg gttccgacac cgtaccttcc ttcagctcga acgcgatctg cgacaacttt
     2221 ggatcgatgg caccttcgcg cacccacccc aggtcgccac ccttcttggc cgagatagcg
     2281 tcttccgata cctgctcggc caccttggcg aaatcggtgc cggcacgaat cttcgatgcc
     2341 gcttcctgcg ccttggtcag ctgcgcgcgg cgctcgttct cgctcatggc gcgcgtggtg
     2401 cggaacagaa tgtgtgcgat atgcacgcgg cgctcggaat aatcctgcgc gtgctggttg
     2461 tagtagctgg tgacggcatc gtcggtgacc tgcttgctca ggtactgctc gaagtagcgc
     2521 gacaccaatg cctcgcggcg gaaatcatct atctcggcct gcaacagcgc ggaatcgacc
     2581 acgtcgctgt gcttcatcgc ctgctccacc gccatggcca ggcctttgcg ctgcaggtac
     2641 tggtcaagcg cgccctgcca gcgcgccgct tccttgtcag tgaccggcag gtgcttgtac
     2701 ttcaggtaag cctgaaattc ctcccgagtc acggcctcgc cgttgacagt tgccagtttg
     2761 tcgccgccac aggccaccag cagacccgcg caggccactg ccagaaacca gttcttcttc
     2821 ttcactcgct cactccctca ctgtaccgct tcattgcaaa tcgagttcgc cgcagtagat
     2881 tcggctggtc agcgaacctc cttgctgcga ggagtcgcag ccaggatcac cgtcgatgac
     2941 cgactccgcg ctgtctcgca ccaccacgcg gtcggtgccg aagtacgcat aaccgccgtc
     3001 ggtgacatcc agccggtaga gagcaaaagg ccgatcaagg tgctaccgca tactgcaacc
     3061 actttccttc caactacttc caaactactc tgacgggatt caccttggat ttcgctcggg
     3121 atttgggacg cgccgttgcg tcatcctatt agtgtctcac tcctaccgat ttttcctcgc
     3181 gcgcaccctc cgcctcgacg cgaatgatca cctcgtcacc cacttccggg agatacgtgg
     3241 tgatgccata gtccgagcgc ttaagcatgc cctgcgcctc aaaaccgcag gcccacacgc
     3301 gcttgatggc gtggatcgcg cagttcatcg ccgtcacctc caacgccagc gggcgcgtca
     3361 cgccatgcag cgtgagctgg ccttcgatgc gggtgatctt gtcgccgctg aagatgactc
     3421 tggtcgagcg atagctcatc tcggggaaat gcgccgcgtc gaggaagtta tccgcgcgca
     3481 ggcgctcgtc gcgcgcgggc atgccgctgt cgagcgacgc caccttcacc gtgacctgga
     3541 ccgagctgcc ctcgcccttg cggtcgagca cgaagctgcc ggcagtctcg ttgaagcggc
     3601 cgtgcatcag cgacaggccc atgtggctga ccgaaaactc gggataggtg tgcgtgggat
     3661 cgagctcgta gcgatctgcc gccatcgcgg ccgccggcag cagcagcgcc agcagccagc
     3721 gtttcacgcg gcttcctcgg gtttgatctt ctcgcgtgcg cggatcatgg cgcgcacgtt
     3781 ctccggcacc ggcagcgatt tctgctgcag gtagctgaac cagacgatgg tgccgcgcga
     3841 gacggcaacg agcttgttgc cgacgaacat gccggcctgc gacgtcatgc tggagcggcc
     3901 gatgcgagcg atgcgcaggc cgatgtcgag cgtcgacgga tgatgcagct gcgcgatgaa
     3961 gtcgcagccg atcgacgcca ggatgatgcc gtagtccttc cagaatttcg gatcgttgcc
     4021 gaacatctgg ccgaagtagt ccagccgcgc cgattcgtcg aaggtgaaaa acacggcgtt
     4081 gttcacatgg cccagcgtgt ccagatcgcc ccagcgcgtg tcgatgcgga tgaagtggga
     4141 gaaatcctcg cgggtgaccg gggcgttcat gcgggctcgc tgctgtcggg ggaggtgccg
     4201 gctggccggc gcgcgattat agccagccgc tgccgcgcga cctccacgat ctgtcgctgg
     4261 cgaacttcgc tggccgtgga ccactgcgcg atttcgtcga ggctgcgccc gcagccgacg
     4321 cacacctgct gcgcgtcgag ctggcaaacc ttgatgcacg gtgattcgat catgactgca
     4381 ttgtaggtgt cgcaaaaaac aaaaaaggcc gcacgcatcg ctgcgtgcgg cctgtattct
     4441 gcggaaaatc agtccaccag gatgcggaac gccacgcggc ggttttcctc gcggccctgc
     4501 tcggtggtgt tgtcggccac cggctccgcc ttgccatagc cgcgcgtctt caggcgctcg
     4561 gccgtcacgc ccttgccgat caggtactgc ttgaccgact cggcgcgctg ctgcgacagc
     4621 atctggttgt acgggtcgct gccagtggtg tcggtgtggc cgccgatctc gacgttgatg
     4681 ttggccgact ccggctgctt cagcgtcgct gccacgccgt cgaggatctt cttcgaatcc
     4741 tcggtcagtt cggcggagtc caggcggaac tggatattgc gcatcagcgc cgcttcaccg
     4801 gccgggcagc cgtgagcatc caccggcgtg ccggcggtgg tgcccggaca ctggtcggcg
     4861 gcatcgggca cgccgtcgtg atcggagtcg acgccacaat cggggcgcgc gccttccggc
     4921 gacaccacgg ccacgccgca ctccggcgcc gccgcggtct tgcccatctt gtcggccagg
     4981 aagtacagcg gcacctggat gccgacgccg acgcgccagt caagcaggct gctctcgctg
     5041 gccaccgact ggtcgttgaa ctggtactgc aaacggccct cggctcgcaa cgccaagccg
     5101 tggaacgagg tcggcaccaa cagtccgccg ccggcgtcga tgccgaaatg ccagtgcgat
     5161 tcgccgagca cgtcttcatg cacggcgtcg aggccggcca gcacgaatgg ctt
//
LOCUS       DASZZC010000756         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1219044,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000756 DASZZC010000000
VERSION     DASZZC010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219044"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHE37_16210"
     CDS             complement(<1..237)
                     /locus_tag="VHE37_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVT37134.1"
                     /translation="MNLFDPLTLRDVTLRNRIAVSPMCQYSADDGAPTDWHLVHLGSR
                     AVGGAGLVMVEATGVTAQGRISPGDAGIYQDAHVP"
     gene            complement(292..777)
                     /locus_tag="VHE37_16215"
     CDS             complement(292..777)
                     /locus_tag="VHE37_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT37135.1"
                     /translation="MALPLEDIELIKRLKYAYCRCIDTANIDELKTLFTEDASVRYVG
                     GSYLFEAQGRDKILEAIRYAFHADAIAFHHVNHPEIDVTSPTEATGVWYLKDWFLDLK
                     NKIITDGSSLYRDTYVKQNGKWLIKRATYERIFEIVTPVTEKPNITAHYLARHGVKHP
                     S"
     gene            complement(946..1707)
                     /locus_tag="VHE37_16220"
     CDS             complement(946..1707)
                     /locus_tag="VHE37_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT37136.1"
                     /translation="MQKFSKGAVLVFGGSGGIGQCVARSFGEAGSDVAVVYRKKKEVA
                     DKVVADIAALGRKASAHLADVTDPAQIAAAVKDAIKTHGRIHTVVWAAGPLVEQVYIG
                     DTSAALWQQSINIEVHGFFNAVQATLPHLRESGGGSYVHLGSAGHAWFPAKDGLSVIP
                     KAANEALCKGIAKEEGKNNIRANSVLVGVIEAGMFLELLKRGVFDQKWTDETQKLLCL
                     KRWGKPEEIGDAAVFLATNGYVTGQQINVSGGFGI"
     gene            complement(1909..2709)
                     /locus_tag="VHE37_16225"
     CDS             complement(1909..2709)
                     /locus_tag="VHE37_16225"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HVT37137.1"
                     /translation="MGETMERIRWGVSLLLLATQAWADADVDRVIACMRANLPVTTLV
                     EQVELSTSDRSGTRVLRGSLYVKGERTPAGARLRAMLRLDAPPDLAGAAYLLRQSADL
                     ASDEVYFYLPAIRRARRVTGASADAPLLGSGFSYNDFRQMQTAFVDGSALMEDPEMID
                     AHPAWVLSFRPPPDEEGHAPYTLVRSWVDQRACVPLKTEFSYGVDIVKQLSASVASLR
                     QFGSTWYAGEVQLRDLHGGGSSTLKVLSASGGAIADAVFDPAIFFSAR"
     gene            complement(2765..>3016)
                     /locus_tag="VHE37_16230"
     CDS             complement(2765..>3016)
                     /locus_tag="VHE37_16230"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HVT37138.1"
                     /translation="RVWVEPKTCLPLKADFYEGGSVRKELTVPVASPKQSGSYWYLSE
                     VQMRDLKDGSGSVLRSTSVSAPKDMPRSNFDPTSFYLGH"
BASE COUNT          496 a          983 c          972 g          565 t
ORIGIN      
        1 cggcacgtgc gcgtcctggt agatgccggc atctcccggc gagatgcggc cctgcgcggt
       61 gacgccggtg gcttccacca tcaccagccc ggcgccgccg accgcgcggc tgcccaggtg
      121 caccaggtgc cagtcggtgg gcgcgccgtc atcggcgctg tactggcaca tcggcgacac
      181 ggcgatgcgg ttgcgcagcg tgacgtcgcg cagggtcagc ggatcaaaca ggttcatcgg
      241 cactccttcg cggcactgcc gctccagcgc ggcagcgcgg agcaaagagt atcaggacgg
      301 atgtttgacg ccgtgcctgg ccagatagtg cgccgtgatg ttcggcttct ccgtcaccgg
      361 ggtgacgatc tcgaagatgc gctcgtaggt ggcgcgcttg atcagccact tgccgttctg
      421 cttcacgtag gtgtcgcggt agagcgagct gccgtctgtg atgatcttgt tcttgagatc
      481 caggaaccag tccttcaggt accacacgcc ggtcgcctcg gtcggcgagg tgacgtcgat
      541 ctccgggtgg ttcacgtggt ggaaggcgat ggcatcggcg tggaacgcgt agcggatcgc
      601 ctcgaggatc ttgtcgcggc cctgcgcttc gaacaggtag gagccgccga cgtagcgcac
      661 gctggcatcc tcggtgaaca gtgttttcag ttcatcgatg ttggccgtgt cgatgcagcg
      721 gcaataggcg tacttcaggc gcttgatcag ctcgatgtct tccagcggca aagccatgtg
      781 cgttctcctc agttattcaa aatttaattt gaccgttgaa agccgttgcg agcgatggcg
      841 gtttgggcgt cgccggagca caggaaccgg agcatatgtg gaaatatgtg aggattccgc
      901 gcaccgccga cgcccaaagc gccgagcgca gcaggcttac aacggtcaaa tgccgaaacc
      961 gccggacacg ttgatctgct gcccggtgac gtaaccattg gtggccagga ataccgccgc
     1021 gtcgccgatc tcttccggct tgccccagcg cttgaggcac agcagcttct gcgtctcgtc
     1081 ggtccatttc tggtcgaaca cgccgcgctt gagcagttcc aggaacatgc ccgcctcgat
     1141 cacgccgacc agcaccgagt tggcgcggat gttgttcttg ccctcttcct tggcgatgcc
     1201 cttgcacagc gcctcgttgg ccgctttcgg aatcaccgag agtccgtcct tggccgggaa
     1261 ccaggcatgg ccggcgctgc ccaggtgcac ataggagccg ccgccgcttt cgcgcaaatg
     1321 cggcagcgtg gcctgcaccg cgttgaagaa gccgtgcacc tcgatgttga tcgactgctg
     1381 ccacagcgca gcgctggtgt cgccgatgta gacctgctcg accagcggcc ccgccgccca
     1441 caccaccgta tggatgcgtc cgtgcgtctt gatcgcatcc ttcaccgctg cggcgatctg
     1501 cgcgggatcg gtcacgtcgg ccaggtgcgc gctggccttg cgcccaagtg cggcgatgtc
     1561 ggctaccacc ttgtcggcca cttccttctt cttgcggtag accacggcca catcgctgcc
     1621 ggcctcgccg aacgagcgcg ccacgcactg gccgatgccg ccgctgccgc cgaacaccag
     1681 caccgcacct ttggaaaatt tctgcatggc tcctccgccg tgatttgcgc gcagggatta
     1741 ttcggagcgc cgcgcggcgc ggcctcatcc gttgggacgg tggcgtacgg gcggaaaatg
     1801 cctgcaccgc atgtcctctt atcgtgttat taaatataat atgtttatta aaatataaaa
     1861 taatttataa cacgcgaaat gcccgtgcgg tggagttgtt tttgcgcttc agcgcgcgct
     1921 gaaaaaaatt gccggatcga acaccgcatc ggcgatggct ccgccggacg ccgacagcac
     1981 cttgagcgtg ctgctgccgc cgccgtgcag atcgcgcagc tgcacttcgc ccgcgtacca
     2041 cgtgctgccg aactggcgca gcgaggccac gctggcggac agctgcttga cgatgtcgac
     2101 gccgtaggag aactcggtct tcagcggcac gcacgcgcgc tggtccaccc agctgcgcac
     2161 cagcgtgtac ggagcgtggc cctcctcgtc cggcggcggg cggaacgaca gcacccaggc
     2221 cggatgcgcg tcgatcatct ccgggtcttc catcagcgcg gagccgtcga cgaacgccgt
     2281 ctgcatctgg cggaaatcgt tgtagctgaa gccgctgccg agcagcggcg cgtcggccga
     2341 ggcgccggtg acgcggcgcg cgcggcggat cgccggcagg tagaagtaca cctcgtcgct
     2401 ggccaggtcg gccgactggc gcagcaggta ggcggcgccg gccagatcgg gcggcgcatc
     2461 gagccgcagc atggcgcgca ggcgcgcacc ggcgggcgtg cgctcgcctt ttacgtacag
     2521 gctgccgcgc agcacgcgcg tgccgctgcg gtcgctggtg gaaagctcca cctgctcgac
     2581 cagcgtggtg accggcaggt tggcgcgcat gcaggcgatg acgcgatcga cgtcggcatc
     2641 ggcccacgcc tgcgtggcca gcagcagcag cgaaactccc cagcggatgc gctccatggt
     2701 ctcccccatg tgcgcatccg atgctacgcc ggggtgcccg gcgcgcatgt cagattttct
     2761 cgactcagtg acccagataa aacgacgtgg gatcgaagtt gctgcgcggc atgtccttgg
     2821 gcgcgctcac gctggtgctg cgcagcacgc tgccgctgcc gtccttgagg tcgcgcatct
     2881 gcacctccga caggtaccag tagctgcccg actgtttcgg cgaggccacc ggcacggtca
     2941 gttccttgcg cacgctgccg ccttcgtaga aatcggcttt caatggcagg caggttttcg
     3001 gctccaccca cacgcg
//
LOCUS       DASZZC010000757         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_757421,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000757 DASZZC010000000
VERSION     DASZZC010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757421"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VHE37_16235"
     CDS             complement(<1..627)
                     /locus_tag="VHE37_16235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HVT37139.1"
                     /translation="MRSCLAAYVLLLVAPAHAQQQEQGNAPAEVAAEPVADNAAAAPA
                     AAPADTIPVAPLHDDTAAATPATEKEAADAQRLDDVVVTATKRPQSVRKIPMSITALT
                     SEKLEEMGAHEIKDFLQLVPGVNTQDEISGIQRKLSIRGVGPDTSTNQTVGIVLGDVP
                     LSDPYGSYTIVDPDPWDLRTVEVLKGPQGTLFGATSLAGLIRYVPNAPE"
     gene            984..2051
                     /locus_tag="VHE37_16240"
     CDS             984..2051
                     /locus_tag="VHE37_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HVT37140.1"
                     /translation="MSTTDFTATAAAAATPSSTVTITVRKPYLLFLGEARDFTEAKTA
                     AGLRDWAADSCMGQWRLPGCGVTLDLPEMQPEQAAARGAGSLVIGIAPVGGRVAPNWI
                     PCLLQAAAAGLDIVSGMHMKLEDVPGLAAVAQARGVRLVNVRVPPAGLPTGNGRKRSG
                     QRLLTVGTDCALGKKYTALALARELRAQGIAADFRATGQTGILIAGSGIPLDAVVADF
                     VAGAAECLSPDAAPDHWDIIEGQGSLFHPAYAGVTLGLVHGSQPDALVLCHDPARIHI
                     HAAPTFVTPPLPVAIERYLDAARLTNPAARMVGIAFNTSALDADARARLLSETEQKLG
                     LPCFDPLKSPLKPVTHRMLQR"
     gene            2048..3031
                     /gene="dgcA"
                     /locus_tag="VHE37_16245"
     CDS             2048..3031
                     /gene="dgcA"
                     /locus_tag="VHE37_16245"
                     /inference="COORDINATES: protein motif:HMM:NF042940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HVT37141.1"
                     /translation="MKRALKIYRHTWPMAEPFTIARGTQTHAEVVVAELAAGAHVGRG
                     EAVGVSYHGETPDALLAQIERVRDAIEAGADRQDLLKLLPAGGARNAVDGALWDLEAK
                     LTGVPAWRRAGLASGGPVTSCITIGIRPLEAYEKAAATLADHPWIKVKVDRTDPLAAV
                     QAVRRGAPKPKLVVDANQSWSVEEVKSFGPQMADLGVALLEQPMAQGPDDALLGVHCP
                     VPICADESISTVDDLPRLAGRYQFINIKLDKTGGLTHALELARAAQKAGLRLMSGCMV
                     GGSVAMAPAMVLAQLCEINDLDGPVLQAADWPGGIVYTRGVMSPPWPQFWG"
     gene            complement(3101..>4127)
                     /locus_tag="VHE37_16250"
     CDS             complement(3101..>4127)
                     /locus_tag="VHE37_16250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649070.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HVT37142.1"
                     /translation="YYSVKTARDSNMRHRPVGLGIMGFNDALYKLRLPYESEEAIRFA
                     DTSMEAVSYYAIKASADLAIERGAYSSFKGSLWSQGILPIDSIQLLAKERGKYLKQDT
                     SVSGTFDWNALRNQVKTTGIRNSNTMAIAPTATIANITGVSQSIEPTYQNLYVKANLS
                     GDFTVVNEYLVNDLKKLNLWDEVMAHDLKYFDGSVQKIDRVPAELKEIYKCAFEIDAK
                     RLVDAGSRRQKWIDQAQSLNLYMAQPSGKKLDELYKHAWLSGLKTTYYLRTLGATHAE
                     KTTISDSRLNKVAGGEGGGVSLKPASAGGRRMSDQPVNDPAVEHIAVAEAPKVCSILD
                     PDCEACQ"
BASE COUNT          649 a         1366 c         1385 g          727 t
ORIGIN      
        1 ttccggcgcg ttgggcacgt agcggatcag cccggcgagc gaggtggcgc cgaacagggt
       61 gccctgcggg cccttgagca cttccaccgt gcgcaggtcc cacggatcgg gatcgacgat
      121 ggtgtacgag ccatacggat cggacagcgg cacatcgccc agcacgatgc ctaccgtctg
      181 gttggtgctg gtgtcggggc cgacaccgcg gatcgacagc ttgcgctgga tgccggagat
      241 ttcgtcctgc gtattgaccc ccggcaccag ctgcaggaag tccttgatct cgtgcgcgcc
      301 catttcctcg agcttctcgc tggtcagcgc ggtgatgctc atcggaatct tgcgcaccga
      361 ctgcgggcgc ttggtggcgg tgaccaccac gtcgtccagg cgctgcgcat cggctgcttc
      421 tttttccgta gcaggcgttg cggctgcggt gtcgtcatgc agcggcgcga ccgggatggt
      481 gtcggccggc gcagccgcgg gcgctgcggc ggcgttgtcc gccacaggct ccgcggccac
      541 ctcggcgggc gcattgccct gttcctgttg ctgcgcatgc gctggcgcca ccagcagcag
      601 gacgtaggcg gccagacacg atcgcaagta acggctcggc actgatattc ccccggcgct
      661 atgccccata agtaccggca gagtctgggc gcggtgcgcg cttgcaggca tccaagaaaa
      721 caccggaacc gcatttttgg cgcgcggcgg ccgtgcaata cctccaccgc aagccatttc
      781 tcgatgttat aaataattta ttataaataa aataatcaaa tattatataa cacaacaacc
      841 cgtcatgcgg tggatgattt tttgcagcgc tgcggtgtcg gacgaattgc agtggcatcg
      901 cttcacgcgt ttttacggat gcgcccggcg cgcgccggct ggtctactgg cacggtcgca
      961 aacccacctc atcgagcacg catttgtcta ctaccgattt cacggcgacc gctgctgcag
     1021 cggccacgcc cagcagcacg gtgaccatca ccgtgcgcaa accttacctg ctgtttctcg
     1081 gcgaagcgcg cgatttcacc gaggccaaga ccgccgccgg cctcagggac tgggccgccg
     1141 attcctgcat ggggcagtgg cgcctgcccg gctgcggcgt gacgctcgac ctgccggaga
     1201 tgcagccgga gcaggcggcg gcgcgtggcg ccggctcgct ggtgatcggc atcgcgccgg
     1261 tcggcggacg cgtggcgccc aactggattc cctgcctgct gcaggcggcc gcggccggac
     1321 tcgacatcgt cagcggcatg cacatgaaac tggaggacgt gcccggactc gccgcggtgg
     1381 cgcaggcgcg cggcgtgcgc ctggtgaacg tgcgcgtgcc gcctgcgggc ctgcccaccg
     1441 gcaacgggcg caagcgcagc ggccagcgcc tgctcaccgt cggcaccgat tgcgcgctgg
     1501 gcaagaagta caccgcgctg gcgctggcgc gcgaactgcg cgcgcagggc atcgccgcgg
     1561 atttccgcgc caccggacag accggcattc tcatcgccgg cagcggcatt ccgctcgatg
     1621 cggtggtcgc cgactttgtc gccggcgcgg ccgagtgcct gtcgcccgat gcagcaccgg
     1681 atcactggga catcatcgaa ggccagggtt cgctgttcca cccggcttat gccggcgtga
     1741 cgctgggcct ggtgcatggc tcgcagccgg acgcgctggt gctgtgccac gacccggcgc
     1801 gcatccatat ccacgccgcg ccgacgttcg tcacgccgcc gctgccggtc gccatcgagc
     1861 gctacctcga cgcggcgcgg ctgaccaacc cggccgcacg catggtcggc attgccttca
     1921 acacctcggc gctcgatgcg gacgcgcgcg cgcgactgct gtcggagacc gagcagaaac
     1981 tcggcctgcc gtgtttcgat ccgctgaaat caccgctcaa gcctgtcacc caccggatgc
     2041 tgcaacgatg aaacgcgcac tcaaaattta ccggcatacc tggccgatgg cggagccttt
     2101 caccatcgcg cgcggcacgc agacgcatgc cgaggtggtg gtggcggaac tcgccgccgg
     2161 cgcccacgtg ggccgcggcg aggcggtggg cgtgagctac cacggcgaaa cgcccgacgc
     2221 gctgctggcg cagatcgaac gggtgcgcga tgccatcgag gccggcgctg atcgccagga
     2281 cctgctcaaa ttgctgcccg ccggcggcgc gcgcaatgcg gtcgacggcg cgctgtggga
     2341 tctggaagca aagctcaccg gcgtgcccgc gtggcgccgc gcaggccttg cgtccggcgg
     2401 accggtcacc agctgcatca ccatcggcat tcgtccactc gaagcctacg agaaggccgc
     2461 agcgactctg gccgatcatc cgtggatcaa ggtgaaggtg gatcgcaccg atccgctggc
     2521 ggcagtccag gccgtgcgcc gcggcgcgcc caagccgaaa ctggtggtcg atgccaacca
     2581 gtcgtggagc gtggaggaag tgaagtcctt cggcccgcag atggcggatc tcggcgtggc
     2641 attgctggaa cagccgatgg cgcaggggcc ggacgacgcg ctgctcggcg tgcactgccc
     2701 ggtgccgatc tgcgccgacg agtcgatcag cacggtcgac gacctgccgc gcctggccgg
     2761 ccgctaccag ttcatcaaca tcaagctcga caagaccggc gggctcacgc acgcgctcga
     2821 actcgcgcgc gccgcgcaga aggcgggcct gcgcctgatg agcggttgca tggtcggcgg
     2881 ctcggtggcg atggcgcccg cgatggtgct ggcgcagctg tgcgagatca acgatctcga
     2941 cggcccggtg ctgcaggccg ccgactggcc gggcggcatc gtctacacgc gcggcgtgat
     3001 gtcgccgccg tggccgcagt tctggggctg agcagcgcgc aataaaaaac cgccctggag
     3061 aacatccagg gcggccgggt ttacagcgca gcgaatgtgc ctattggcaa gcctcgcaat
     3121 ccggatcgag gatcgagcac accttcggtg cttccgccac ggcgatgtgc tccaccgccg
     3181 gatcattcac cggctgatca ctcatgcggc ggccgccggc tgacgcaggc ttcaggctca
     3241 cgcccccccc ttcaccaccc gccaccttgt tcaggcgcga gtcgctgatg gtggtcttct
     3301 ccgcgtgcgt ggcaccgagc gtgcgcaggt agtaagtggt cttcaggccc gacagccagg
     3361 cgtgcttgta cagctcgtcg agcttcttgc cggagggctg cgccatgtac aggttgaggc
     3421 tctgcgcctg gtcgatccac ttctggcggc ggctgccggc gtcaaccagt ctcttcgcgt
     3481 cgatctcgaa ggcgcacttg tagatttcct tcagttcggc cggcacgcgg tcgatcttct
     3541 gcacgctgcc gtcgaagtac ttgaggtcgt gcgccatcac ctcgtcccac aggttcagct
     3601 tcttcaggtc attgaccagg tactcgttga ccacggtgaa gtcgccggag agattggcct
     3661 tgacgtagag gttctggtag gtgggctcga tcgactgcga cacgccggtg atgttggcga
     3721 tggtcgcggt cggcgcgatg gccatggtgt tgctgttgcg gatgcccgtc gtcttcacct
     3781 ggttgcgcag cgcgttccag tcgaaggtgc cgctgacgct ggtgtcctgc ttgaggtact
     3841 tgccgcgctc cttggccagc agctggatgg agtcgatggg caggatgccc tggctccaca
     3901 gcgaaccctt gaagctggag taggcgccgc gctcgatggc aaggtcggcg ctggccttga
     3961 tcgcgtagta gctcaccgct tccatcgagg tgtcggcgaa gcggatcgcc tcctcgctct
     4021 cgtacggcag gcgcagcttg tacagcgcgt cgttgaaacc catgatgccc aggcccaccg
     4081 ggcggtggcg catgttggaa tcgcgcgcgg tcttcaccga gtagtag
//
LOCUS       DASZZC010000758         8042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_438508,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000758 DASZZC010000000
VERSION     DASZZC010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438508"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..993)
                     /locus_tag="VHE37_16255"
     CDS             complement(52..993)
                     /locus_tag="VHE37_16255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT37143.1"
                     /translation="MSEIELHRKLLGDAARNAAFHAALQKVIVPGKSTVADLGAGTGF
                     LSFLALQLGAKHATLVEYSETLGLAEYLARKNKLTALTFMHMHSSEVKKPPKCDVVVS
                     ETLGNYALEENLLETLVDARRWLAPGGTLIPARLKQYVAPVMHARLQDEIDVWPRVGY
                     GLDLTPARAVALNNMYVKRIMPDDLGPAGGAPGLWDDIDFNPKAAAPSSQREGTVQWR
                     PDSALTIHGYALWWECELLPGISIGTSPYAPATHWEQVYLPLMRPLEVRADDTLEVKL
                     TSDTRPQVGVRVCWRTRLTRAGKSVSEQSQDTLRGRL"
     gene            1025..3478
                     /gene="hrpB"
                     /locus_tag="VHE37_16260"
     CDS             1025..3478
                     /gene="hrpB"
                     /locus_tag="VHE37_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HVT37144.1"
                     /translation="MKLPDLPVAEVLPALARALGGDGRAVLAAPPGSGKTTLAPLALL
                     DEAWLRGKKIVMLQPRRVAARASASRMAQLLGEKVGDTVGYQIRFERRIGPATRIEVI
                     TEGLLARRLQSDPELPGVGLVIFDEFHERSLDADLALALALDARANLNPELRVLVMSA
                     TLDTQRVSALLGGASIIESDGRLHPVDVRYRHARADAAVEDSVAAGVLEALAETSGDI
                     LAFLPGAREIRRCGERLAGRIGSARVMPLYGELSSAEQDAALAPARDGVRKIILATNI
                     AQTSITVEGVTTVVDGGYARVARFDLGAGENRLDTGRVSRASADQRAGRAGRLGPGVA
                     YRMWSREQHGLLAPHDTPEILASDLSRFALELAGWGTTPDALALLDAPPAAAWSYARD
                     LLTDLGALDAQGRITPHGRELARLPASPRRAHMLLAARALGAAPLAAWIAAVLDERGT
                     SLDLGPTVRAYLQGRAPDAGAQRRVRETAKQLARLIDAGEEARYDDRAAARAVSLAYP
                     ERIARRRERPGVFLCEDGAEARVPETDDLAHAPWIAIAHWDPKPPRKVRAALALDEAD
                     LKSDHAARIRRIADVRWDAQSEAVVAEEQLRLGAIVLERRALRDSAGDAARAAMLAGI
                     RALGLHALPWTPAAREWQARVLSLRAWRGEEWPDVSDEALLATLDDWLGPHLDGITRR
                     AHLDRLDLPTILGSRFDYARQQLLNRLAPTHIEVPTGSHIRLDYKPGQAPALEVKLQE
                     MFGCRDTPTVNDGRVKVVLHLLSPGQKPIAVTADLAGFWARGYADVKKDMKGRYPRHP
                     WPDDPLSALPTRRAKPRGT"
     gene            3560..3645
                     /locus_tag="VHE37_16265"
     tRNA            3560..3645
                     /locus_tag="VHE37_16265"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3594..3596,aa:Tyr,seq:gta)
     gene            3687..3760
                     /locus_tag="VHE37_16270"
     tRNA            3687..3760
                     /locus_tag="VHE37_16270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3719..3721,aa:Gly,seq:tcc)
     gene            3793..3868
                     /locus_tag="VHE37_16275"
     tRNA            3793..3868
                     /locus_tag="VHE37_16275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3826..3828,aa:Thr,seq:ggt)
     gene            complement(4214..7990)
                     /locus_tag="VHE37_16280"
     CDS             complement(4214..7990)
                     /locus_tag="VHE37_16280"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVT37145.1"
                     /translation="MNFFDRTLASVHKVVVAALAVALGSMGFAVAAPYPDGPPNDASY
                     GQQHNVWDYIPAQTYPLATDAEGAAGMSIEKAWRDPLLAGARTGRKDLMVAYLEGGIN
                     WNLGDISKDLVNKVYLNRGELPCPRDAAGKTRSQLDPSATDCYDLNGDGVFNIQDYAS
                     DGRVSDRNGNGFLDPEDLIAIFSDGTDDDGNGYVDDISGWDFYNNQNDPATLDATYPH
                     SDAQMRKAAAEANNGLGFAGMCPDCRILPVKVGAEALDRADDLARGILYAIDAGASVV
                     TTENAVLGYSTLERQVLEYAWQHDVLIAETTNDFDSTDHQGAMYWPHDIPGNGLVADQ
                     SSLPVISSPVQPTTALTTTFRVRSGQTSWGTKSLLSVATLGGSTSESTGTLGGVLGML
                     RSAAKEFSPAANPITAAEVVQVAIATASDVTASDGAKWPVTQGWDLQTGYGRYNVWEA
                     MDAMRRQDIPPVAWFDTPDWFTLYDPTQVGTVAIGGHVEARRSPSYQWKLEWATGGEP
                     ADDSWQTISHGAGQVPYSGELGVLDLSRISAAFYAAQFALSSTKTLETNEQYTVTLRL
                     TVTDAAGKSGVERRSIAVHHDDSWFPGFPVRTRETGTSFAPGGESQPALVDLQGLGHL
                     AIVYGDSDGHIHALDPLTRKELPGFPATTDPTELAPVKPRAGIEASFEPMPQPVAVGD
                     LDHDGRLWIVGTTTNGKVYVWDDHGARRAGWPQLAATGVQPPAVPRQPRQYARPPHQG
                     AFAAPTLSDLDGDGTLEIIQAAWDGRIHVYRNDGSGELPGWPVKVELPSSSAPPQGYV
                     LIDDQKLESNVLVADIDGDGKPELVVRSQQAYSRGGDIQPLGLGHAFAYHADGTLLSG
                     WPVTMPSVIHYYGSAQEFITEGSNSPVGADIDGDGKVDVAVNPVFSPAVYVFNGDGTP
                     KLAYGAALDLSNHAVTQADLSNLLGLLPADLSISFTTTGAFGKFGPGKLLTFAQAGSD
                     TASVANALLFTGSGGAIRNSERAFVAAAGVPQPGFPSMLQGLDFLGAPLFVDVDGDGA
                     AEIIDGGDTSALHGFRSGGGQAAGFPKWTTGWMLWSASAGDLDSDGTTDLVALTREGY
                     VMAWKTPGISASNNEWWHYRHDEWNTGRYGADTRPPGVARDVKWDVANHRLSFTAPGD
                     NWYAGTVSKYRVAGTGTAGVSTELEFSSTDFVPAGQQQSLNLPADMTSGTVQAVDAAG
                     NLGRRVAFPGSSNLPNGASDTPAERSRFGGPISAWSTGILLLVQAFRRRRIRPG"
BASE COUNT         1303 a         2707 c         2638 g         1394 t
ORIGIN      
        1 cccccccccc ccgctcctac acatcgcctg cccggcgcac ctgtcgcgcg cctacaaccg
       61 cccgcgcagc gtgtcctggc tctgctcgct cacgctcttg cccgcgcgcg tgagccgcgt
      121 gcgccagcac acgcgcacgc cgacctgcgg ccgcgtgtcc gaggtcagtt tcacttccag
      181 cgtgtcgtca gcgcgcactt caagtggacg catcaacggc aggtacacct gctcccagtg
      241 cgtcgccggc gcgtatggag atgtgccgat ggaaatgccc ggcagcagtt cgcactccca
      301 ccacagtgcg tagccgtgga tggtgagcgc gctgtccggg cgccactgca cggtgccttc
      361 acgctggctc gacggcgcag ccgctttcgg attgaagtca atgtcgtccc acaaacccgg
      421 cgcgccgccg gcggggccga gatcgtcagg catgatcctc ttcacgtaca tgttgttgag
      481 cgccaccgcg cgcgcgggcg tgagatctag cccgtagccg acgcgcggcc acacgtcgat
      541 ctcgtcctgc agccgcgcgt gcatcaccgg cgccacgtac tgcttcagcc gcgcggggat
      601 cagcgtgccg ccgggcgcca gccagcgccg cgcatcgacc agcgtctcca gcaggttttc
      661 ctccagcgcg taattgccca gcgtttccga tacgaccaca tcgcacttcg gcggcttctt
      721 cacctcgctc gaatgcatgt gcatgaaggt gagcgcggtg agcttgttct tgcgcgccag
      781 gtattcggcc agccccagcg tctcgctgta ttcgaccagc gtggcgtgct ttgcgcccag
      841 ctgcagcgcg aggaacgaga ggaaaccggt gccggcgccg aggtcggcca ccgtgctctt
      901 gcccggcacg atcactttct gcagcgcggc gtggaacgcg gcattgcgcg cggcgtcgcc
      961 gagcagcttg cggtggagtt cgatttcgct catggcgcca ttctccccga gaatatgcgc
     1021 agccgtgaaa ttgcccgacc tgcccgtcgc cgaagtgttg cctgcgctgg cccgcgccct
     1081 gggcggcgac ggccgcgccg tgctcgccgc gccgccgggc tcgggcaaga ccacgctggc
     1141 gccgctggca ttgctcgacg aagcatggct gcgtggcaag aaaatcgtga tgctgcagcc
     1201 gcgccgcgtg gccgcgcgcg cctcggcctc ccgcatggcg caattgctcg gcgaaaaagt
     1261 cggcgacacg gtgggctacc agatccgctt cgagcggcgc atcggcccgg ccacgcgcat
     1321 cgaggtgatc accgagggcc tgctggcgcg ccgcctgcag tccgacccgg aactgccggg
     1381 cgtgggcctg gtgatcttcg acgaattcca cgagcgcagc ctcgatgcgg acctggcgct
     1441 ggcgctggcg ctcgacgcgc gcgccaatct caatcccgag ctgcgcgtgc tggtgatgtc
     1501 ggccacgctc gacacgcaac gggtgtccgc gctgctcggc ggcgcaagca tcatcgagag
     1561 cgacggccgc ctgcacccgg tcgacgtgcg ctaccgccac gcgcgcgccg atgcggcggt
     1621 ggaggacagc gtggcggccg gcgtgctcga ggcgctggcg gagacctccg gcgacatcct
     1681 cgccttcctg cccggcgcgc gcgaaatccg ccgctgtggc gagcggctgg ccgggcgcat
     1741 cggcagcgcg cgcgtcatgc ctttatatgg agagctgtcg tccgccgagc aggacgccgc
     1801 gctcgcgccc gcgcgcgatg gcgtgcgcaa gatcatcctg gccaccaaca tcgcgcagac
     1861 cagcatcacg gtggaaggcg tgaccaccgt ggtcgacggc ggctatgcgc gggtggcgcg
     1921 tttcgatctc ggcgctggcg aaaaccggct ggacaccggg cgcgtgtcgc gcgccagcgc
     1981 cgaccagcgc gcgggccgcg cgggccgcct gggtccgggc gtggcgtatc gcatgtggag
     2041 ccgcgagcag catggtttgc tggcaccaca cgacacgccg gaaatcctcg cgtccgacct
     2101 gtcgcgcttc gcgctcgagc tcgccggctg gggtacgacg cccgatgcgc tggcgctgct
     2161 cgatgcgccg ccggccgccg cgtggagcta tgcgcgtgac ctgctcaccg acctgggcgc
     2221 gctcgatgcg cagggccgca ttacgccgca cgggcgcgag ctcgcgcgcc tgccggcgtc
     2281 gccgcgccgc gcacacatgc tgctggccgc gcgcgcgctc ggtgccgcgc cgctggccgc
     2341 gtggattgcc gcggtgctcg acgagcgtgg gacttcactc gatctgggcc ccaccgtgcg
     2401 cgcgtacctg caagggcgcg cgcccgacgc cggcgcgcag cggcgcgtgc gcgagacggc
     2461 gaagcagttg gcgcggttga tcgatgcagg cgaagaagcg cgttatgacg accgcgccgc
     2521 cgcgcgcgcg gtgagcctgg cctatcccga gcgcatcgcg cgccgccgcg aacgcccggg
     2581 cgtgttcctg tgcgaggacg gtgccgaggc gcgcgtgccg gaaaccgacg acctggcaca
     2641 tgcgccgtgg atcgccatcg cgcactggga tccgaagccg ccccgcaagg tgcgcgccgc
     2701 actggcgctg gacgaagccg acctgaaatc cgatcacgcc gcacgtatcc gcaggattgc
     2761 cgatgtgcgc tgggatgcgc agagcgaggc ggtcgttgcc gaggaacagc tgcgcctggg
     2821 tgccatcgtg ctcgagcgcc gcgcgctgcg cgacagtgcc ggcgacgctg cgcgcgctgc
     2881 catgctcgcc ggcatccgcg ccctgggcct gcacgcgctg ccgtggacgc cggcggcgcg
     2941 cgagtggcag gcgcgcgtgc tcagcctgcg cgcgtggcgt ggggaagagt ggccggatgt
     3001 atcggacgag gccctgctcg ccacgctgga cgactggctt ggtccgcacc tggacggcat
     3061 cacgcgccgc gcgcatctcg accggctcga tctgccgacc atcctcggca gtcgtttcga
     3121 ctatgcccgg cagcaattgc tcaaccgcct tgcgcccacg catatcgagg tgccgaccgg
     3181 gtcgcacatc cggctggact acaagcccgg ccaggccccg gcgctggaag tgaaactgca
     3241 ggaaatgttt ggctgccgcg acacgcccac ggtcaacgat ggccgcgtga aagtggtgct
     3301 gcacctgctc tctccggggc agaagcccat cgcggtgacc gccgaccttg ccggtttctg
     3361 ggcgcgcggc tacgccgatg tgaagaagga catgaagggc cgctacccgc gccacccgtg
     3421 gccggacgac cccttgtccg ccctgccgac ccggcgggcc aagccgcggg gcacctgagt
     3481 acaaatttgg cttgacgccc ccgggaggcg cacggacaat acgcgccctt ttggccccgc
     3541 ccgggccgga aggaacggcg gagggatact caagcggcca acgagggcag actgtaaatc
     3601 tgctggctta tgcctacgaa ggttcgaatc cttctccctc caccagcttg agccaggctc
     3661 cgcgcggcgg taaaaaaggc agacgcgcgg gtgtagttca atggtagaac ttcagccttc
     3721 caagctgata gcgtgggttc gattcccatc acccgctcca gtttctgcct tgtttcaagc
     3781 gccagacaaa gcgcccacat agctcagtcg gtagagcact ttcttggtaa ggaagaggtc
     3841 atcggttcga ttccgattgt gggcaccaac tacgaagccg gcgcaagccg gcttgtttat
     3901 ttagatcgcc ggatttctta ggcgctgatt cggcgaaagc cggactcatt tccattttcc
     3961 gggttcggcg ctggcgatgc tgggcatcta tagggcgtcg atcctgcact catcggtgcc
     4021 cgagatttct tccggcactc aagattgacc tccattcctg gcgctcatct ccagcttcac
     4081 tttgcgcttc gccccgcgtt tcttgggcga ttcccggtct ggaacgtatg cgatcaggtc
     4141 ggccgctcca atctggaata gctgcagagt gggtccgaag tttcgacgtc gacgcgcgtc
     4201 tgcatcgcaa tgttcacccg ggtctgatgc gtcgtcgcct gaacgcctgc accaacaaca
     4261 ggattcctgt cgaccaggct gaaattggtc cgccaaaacg cgagcgctcc gccggggtgt
     4321 cggacgcgcc gttcggcagg ttgcttgagc ctggaaatgc gaccctgcgc cccagatttc
     4381 ccgccgcatc gacggcctgc acggtcccgc tcgtcatgtc agccggaagg ttgagcgact
     4441 gctgttggcc agccggcacg aaatcagtcg atgaaaattc cagctcggtc gagacgccgg
     4501 cggtgccggt acctgcgacg cgatacttgg acaccgtgcc ggcgtaccag ttgtctcccg
     4561 gtgccgtaaa cgacagtcga tgattggcaa catcccactt cacatcacga gcgacacctg
     4621 gcgggcgggt gtccgcgccg tagcgccctg tgttccattc gtcatggcgg tagtgccacc
     4681 actcgttgtt ggacgccgaa atgcccggtg tcttccatgc catgacataa ccttcacgcg
     4741 tcaatgccac cagatccgta gtgccatcgg aatcaagatc gcccgcggat gccgaccaca
     4801 gcatccaccc ggtcgtccat ttcgggaagc ccgcagcctg cccgccgccg gaacggaagc
     4861 catgcagggc actcgtgtcg ccgccatcga tgatttccgc ggcgccgtcg ccgtcaacgt
     4921 cgacaaacag cggcgcaccg aggaagtcga gtccctggag cattgacggg aacccgggct
     4981 gcggaacgcc ggccgcggcc acgaatgcgc gctcgctgtt cctgatggcg ccgcccgatc
     5041 ccgtgaacag cagggcattg gcgacagacg ccgtatccga acctgcctgg gcaaaggtca
     5101 gcagcttgcc ggggccaaac ttgccgaacg cgccggtggt ggtaaatgag atcgacaggt
     5161 cagccggcag caaccccagc aggttgctca gatcggcctg ggtgacggcg tgattgctca
     5221 gatccagggc ggcgccgtaa gcgagcttgg gcgtaccgtc accgttgaac acgtacacgg
     5281 caggcgaaaa gaccgggttg accgcgacgt cgaccttgcc gtcgccgtcg atgtcggcac
     5341 ccaccggtga attcgagccc tcggttatga actcctgcgc gctgccgtag tagtgaatca
     5401 cgctgggcat ggtcaccggc cagccggaca gcagggtgcc gtcggcgtga taggcgaaag
     5461 cgtggcccag gcccaggggc tggatgtctc cgccgcgtga ataggcctgt tgcgagcgca
     5521 ccaccagttc aggcttgccg tcgccgtcga tgtcagcgac cagcacgttg gattccagct
     5581 tctggtcgtc gatcagcaca tacccctgcg gaggcgcgct tgaagacggc aattccacct
     5641 tgaccggcca gccgggcaat tccccgctgc catcattgcg gtagacgtgg atgcgtccat
     5701 cccacgccgc ctggatgatc tccagcgtgc catcaccatc gagatcgctg agcgtcggcg
     5761 cggcaaaagc accctgatgc ggcggacgcg cgtactgccg gggctggcgc gggacggcgg
     5821 gcggctgcac accggtggcg gcgagttgcg gccaaccggc gcggcgcgca ccgtgatcgt
     5881 cccacacata aaccttgcca ttggtcgtcg tgcccacgat ccacagacgt ccgtcgtggt
     5941 cgaggtcgcc taccgcaaca ggctgcggca tcggttcaaa cgatgcctcg atcccggcgc
     6001 gcggtttcac tggcgccagt tcggtgggat ccgtggtagc gggaaatccg ggcaattctt
     6061 ttcgagtgag cggatccagt gcatggatat ggccgtcgga atcaccataa acgatggcca
     6121 gatggccgag gccctgcaga tcgacgagtg cgggctgcga ttcgcctccc ggggcaaagc
     6181 tcgttccggt ctctcgtgtc cgcaccggga agcccgggaa ccagctgtcg tcgtgatgaa
     6241 ccgcgatgct gcgccgctcg acgccgctct tgcccgcagc gtccgtcacg gtcagacgga
     6301 gcgtcaccgt gtactgctca ttggtttcca gtgtcttggt ggatgacagc gcaaactgcg
     6361 cggcatagaa cgcggcggat atcctggaca gatccagcac gccaagctct ccgctataag
     6421 gtacctggcc ggctccatgc gagatcgtct gccagctgtc atcagctggc tctccccccg
     6481 tcgcccactc cagtttccat tgatagctcg gcgagcggcg tgcctcgaca tgtccgccaa
     6541 tggcgaccgt gccgacctgc gtgggatcgt aaagcgtgaa ccagtccggt gtgtcgaacc
     6601 atgccaccgg gggaatgtcc tgccggcgca tggcgtccat cgcttcccac acgttgtagc
     6661 ggccgtatcc ggtttgcagg tcccagccct gcgtgacggg ccacttggcg ccgtcggacg
     6721 ccgtgacatc ggaggcggtc gcgatcgcga cctggaccac ctccgctgcc gtgatcggat
     6781 tggccgctgg cgagaactcc ttggcggccg agcgcagcat gcccagcacg ccacccagcg
     6841 tcccggtcga ttccgaagtt gatcccccga gcgtggcgac cgaaagcagg ctcttggtgc
     6901 cccatgaagt ttgccccgac ctcacccgga acgtagtggt gagcgcggtg gtcggctgaa
     6961 ccggtgaact gatgacgggc agagaactct ggtccgcgac caggccattg ccgggaatgt
     7021 catggggcca gtacatggcg ccctggtgat cggtcgagtc gaagtcgttg gtcgtttcgg
     7081 cgatcagcac gtcgtgctgc caggcgtact caagcacctg ccgctcgagg gtcgagtagc
     7141 ccaggacggc attttccgtc gtaaccaccg atgcaccggc gtcaatcgca tacaggattc
     7201 cgcgcgccag gtcgtcggcg cggtccaggg cttcggcgcc gaccttgacc gggagaatgc
     7261 ggcagtcagg gcacatgccc gcgaagccga ggccattgtt ggcttccgcc gcggccttgc
     7321 gcatctgcgc atccgagtgc gggtaggtgg cgtccagcgt cgccgggtca ttctggttgt
     7381 tgtagaagtc ccagcccgag atgtcatcga cgtagccgtt gccgtcgtca tcggttccat
     7441 cgctgaaaat ggcaatcagg tcttcgggat caagaaagcc attgccgttg cggtcggaga
     7501 cgcggccgtc gctggcgtag tcctggatat tgaaaacgcc gtccccgttg aggtcgtagc
     7561 agtccgtcgc agacggatcc agctggctgc gcgtcttgcc ggcagcatcg cgcgggcatg
     7621 gcagttcgcc ccggttgagg tagaccttgt tgaccaggtc tttcgaaatg tcgccgaggt
     7681 tccagttgat gccgccctcg agataggcca ccatcaggtc cttgcggccg gtgcgcgctc
     7741 cggcgagcaa cggatcgcgc caggcctttt cgatcgacat gcccgccgca ccttcggcgt
     7801 cagtggccag cggataggtc tgggcgggaa tgtaatccca gacattgtgc tgttggccat
     7861 aacttgcgtc gttcggcggc ccatcggggt acggagctgc gactgcgaat cccatgctgc
     7921 ccagggcaac tgccagggcg gcgacgacga ccttatggac cgatgccaaa gtccgatcga
     7981 agaaattcat gaaaagactc cccttaacgt tgccgacttt tgatttcacc gccggatggt
     8041 gc
//
LOCUS       DASZZC010000759         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_321102,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000759 DASZZC010000000
VERSION     DASZZC010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321102"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHE37_16285"
     CDS             complement(<1..237)
                     /locus_tag="VHE37_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HVT37146.1"
                     /translation="MKQVVIYTDGACRGNPGPGGWGAVLQYNGREKRLKGGETPTTNN
                     RMEMTAAIRALEELREPCAVTLYTDSTYVMKGLTE"
     gene            complement(234..1013)
                     /locus_tag="VHE37_16290"
     CDS             complement(234..1013)
                     /locus_tag="VHE37_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVT37147.1"
                     /translation="MAVTRFSRGGLPLTRHALATWLASPRGRRVMDMEQAEMRRLLPD
                     VFGRHVLQIGSWGQQGELIGGAETLHRAVLATVRDDACASAVIDPENLPLPANSVDAV
                     ILPHSQEFARSPHNVLREASRVLNERGKLFVFGFSPWSGWALLRLLGFGYRAFPSGAR
                     FHGAGRICDWLELLDFEITEVRRFGTGFPWLKSRSEGEAWNLANLVYPFAEAYLLVAK
                     KRVLPMNFVGRVQRAQVRPLVGVPVPAPGAQSRANCEQPDS"
     gene            1038..1835
                     /gene="gloB"
                     /locus_tag="VHE37_16295"
     CDS             1038..1835
                     /gene="gloB"
                     /locus_tag="VHE37_16295"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HVT37148.1"
                     /translation="MRTMIAMFAITAIPAFTDNYLWLLQHDRRAVVVDPGDAQPVLKE
                     LQARGLELDAILVTHWHPDHIGGLGALTARYPVPVYGPRGESAKIAGLTRWLGEGERV
                     SVLGQFEFQVLEVPGHTLGHIAYYCAAAGILFCGDTLFSAGCGRLFEGTPAQMHASLA
                     RLSALPPETRVYCTHEYTLSNLAFAHEVEPANRALAARIAEVRSLREQNLPSLPSSIE
                     AELKFNPFLRCDQPEVRAAAWRRAKKDVEHPADVFAVLRAWKDGYKG"
BASE COUNT          334 a          708 c          676 g          311 t
ORIGIN      
        1 ctcggtgagt cccttcatca cgtaggtgga gtcggtatag agcgtcaccg cgcagggctc
       61 gcgcaactct tccagcgcgc ggatcgccgc ggtcatttcc atgcggttgt tggtggtcgg
      121 cgtctcgccg cccttgagcc gcttctcgcg gccgttgtac tgcagcacgg cgccccagcc
      181 gccggggccc gggtttcctc tgcaggcgcc gtcggtgtag atgacgacct gcttcacgag
      241 tcgggctgct cgcagttggc gcgcgactgc gcgcccggcg cgggcaccgg cacgcccacc
      301 agcggacgca cctgcgcgcg ctggacgcgg ccgacgaagt tcatcggcag cacgcgcttc
      361 ttggccacca gcagatacgc ctcggcgaac ggataaacca ggttggccag gttccaggcc
      421 tcgccttcgc tgcgcgattt cagccaggga aaaccggtgc cgaagcggcg cacctcggtg
      481 atctcgaaat ccagtagctc gagccagtcg cagatgcggc cggcgccgtg gaagcgcgcg
      541 ccgctgggga aagcgcggta gccaaaaccg agcagccgaa gcaatgccca gccgctccac
      601 ggtgaaaaac cgaacacgaa aagcttgccg cgctcgttga gcacgcggct cgcctcgcgc
      661 agcacgttgt gcggcgagcg cgcgaattcc tggctgtgcg gcaggatcac cgcatccacc
      721 gagttcgccg gcagcggcag gttttccggg tcgatcaccg ccgaggcgca ggcgtcgtcg
      781 cgcacggtgg cgagcacggc gcggtgcagc gtctcggcgc cgccgatcag ctcgccctgc
      841 tggccccacg agccgatctg cagcacgtgg cggccgaata catccggcag caaccggcgc
      901 atctcggcct gctccatgtc catcacccgg cggccacgcg gcgaagccag ccacgtcgcc
      961 agcgcgtggc gcgtgagcgg caggccgccg cgcgagaagc gggtcacagc catggcgaat
     1021 tgacgggtct aaaactcatg cgcaccatga tagcgatgtt cgccatcacc gccatccctg
     1081 ctttcaccga caactacctg tggttgctgc agcacgaccg gcgggcggtg gtggtcgacc
     1141 ccggcgacgc gcaaccggtg ctgaaagagt tgcaggcgcg cggcctggaa ctcgacgcca
     1201 tcctggtcac ccactggcac cccgaccaca tcggcgggct gggcgcgctg actgcacgct
     1261 acccggtgcc ggtgtacggt ccgcgcggcg agagcgcgaa gatcgccggc ctcacgcgct
     1321 ggcttggcga aggcgaacgc gtgagcgtgc tcggccagtt cgagttccag gtgctggaag
     1381 tgccgggcca tacgctgggc catatcgcct actactgcgc ggcggccggc atcctgttct
     1441 gcggcgacac gctgttctcc gccggctgcg ggcggctgtt cgagggcacg cccgcacaga
     1501 tgcacgcctc gctcgcgcgc ctctccgcgc tgccgccgga aacgcgcgtg tactgtacgc
     1561 acgagtacac cctgtcgaac ctggctttcg cgcacgaagt ggaacctgcc aaccgcgcgc
     1621 tggcggcgcg catcgccgag gtgcgcagcc tgcgcgagca aaacctgccg agcctgccca
     1681 gcagcatcga agcggagctg aagttcaatc ccttcctgcg ctgcgaccag cccgaagtgc
     1741 gcgcggcagc atggcgccgg gcaaagaagg atgtcgagca tcctgcggac gtgttcgcgg
     1801 tgttgcgtgc gtggaaagac ggttacaagg gttgaaatct cctgctcgct gacgctcgcc
     1861 gccctctttt ccaaagaggg cttgcagcag cgcgccttgc tcacgatctg cccctgaaat
     1921 cgcgcgcaag gcgcgctcct acagaggtga accctgcaac gcaagctgcc ccgagcggcc
     1981 cgggatttcc ccgaaggcga cgtgatgacg cggcagccgc gcgcgatcc
//
LOCUS       DASZZC010000760         5402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_62436,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000760 DASZZC010000000
VERSION     DASZZC010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62436"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /gene="lysS"
                     /locus_tag="VHE37_16300"
     CDS             <1..1282
                     /gene="lysS"
                     /locus_tag="VHE37_16300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVT37149.1"
                     /translation="VMGKASFGTLADMSGRIQVFAQQQALGEDTYNVFKTADVGDILG
                     AVGTVMKTKTGELSLRVSELRLLAKGVRPLPEKWAGLTDTEQRYRQRYVDLIVNPDVR
                     AVFEKRGQAVRFIRSFFDALGFVEVETPMLQPIPGGATARPFITHHNALDRDLYLRIA
                     PELYLKRLVVGGFERVYEINRNFRNEGLSTRHNPEFTMLEFYQAYADYRDLMDLIETL
                     LRDLAITVAGSGKLIYQERGYDLEKPFTRWTMRQAVKHFNQALDEAKLGERDYLAQAA
                     RALRIEVKDGYGAGKILTEIFEKTAEANLLDPTFITEYPTEVSPLARQNDADPTITDR
                     FELFVGGREIANGFSELNDPDDQAARFRAQVAAKDRGDSEAMHYDADYIRALEYGMPP
                     TAGAGIGIDRLVMFMTDSPSIRDVLLFPQMRPEV"
     gene            1323..2561
                     /locus_tag="VHE37_16305"
     CDS             1323..2561
                     /locus_tag="VHE37_16305"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HVT37150.1"
                     /translation="MKQASLTDLRTQYVHPPLRWMLMLAMISAIGPMAIDMYLPSLPA
                     LQRHFHSDTAATQLTLSAFFIGLACGQIMYGPVSDRFGRKPPLLFGLGLFTLVSLACV
                     FAPSIEALTLLRFAQALGGAGGMVIVRAIIRDRYPPQDMARVLSLLLLVMGVAPILAP
                     LAGGWVFQWWGWQAIFIVLTAYGALCFAAVACGLTETIVDRAPALRMSAIAIGYGRML
                     AHRRFLGYALAGGVAQGGMFAYISASPFVFIDFFGLSPTHYGWVFGTNALGLISASQI
                     NTRVLRRLPAQQVLRRAIRSYAGFGLLLLLCAWRGWGGVWGVLIPLVCCISSLGFTFP
                     NSTAAAMAPFGDRAGSASALLGTLQFAIAACTGALVGHLLRNSAVPMAAVIAGCGCTS
                     AALLAWVRPHESHKPVRGDS"
     gene            complement(2625..3101)
                     /locus_tag="VHE37_16310"
     CDS             complement(2625..3101)
                     /locus_tag="VHE37_16310"
                     /inference="COORDINATES: protein motif:HMM:NF023510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HVT37151.1"
                     /translation="MAELECGHRQHVRHQPPGINRPWVTTPRCTRPHPVIARRHPAHR
                     SLGRSRAFPRAGWFIAATHWRDLIPAPSCSTWKEAARMHRCSICSAPTSREPGRGCWR
                     RAASWLRKMRRCRAAIWKTCSAWCSTSAPRIPAPGDSMRPRRRRFFGPTAHRLLML"
     gene            complement(3195..3572)
                     /locus_tag="VHE37_16315"
     CDS             complement(3195..3572)
                     /locus_tag="VHE37_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT37152.1"
                     /translation="MKRVTGIGGIFFSAKDPAKLCAWYKTHLGIDVQAWGGAAFDWID
                     DQGAPVAGTTAWSIGDGQRFAPGKTFMVNYRVADLRGLLKSLREEGCQVLDQVDESEF
                     GIFGWVMDPEGNKVELWQPPAGQ"
     gene            complement(3828..4352)
                     /locus_tag="VHE37_16320"
     CDS             complement(3828..4352)
                     /locus_tag="VHE37_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37153.1"
                     /translation="MVRTYRFFAIFGLVFTLASAHAEGTPIVVDSNGTIVGPIVSIAD
                     NIYPHDADGNAAVLMTLNNHTFGVIVSRRGFNGPPQPIVFESSDCSGTPYLGYDTETE
                     NEWANLLILPGFVAGKTETVFVPTRQSPVPVTAQSALTANSQCVASPGHFVMVKGQRV
                     GSLHNAFVPPFSVQ"
     gene            complement(4962..>5402)
                     /locus_tag="VHE37_16325"
     CDS             complement(4962..>5402)
                     /locus_tag="VHE37_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37154.1"
                     /translation="VRHMKPEQVETIALPNKAGTVRIEWYGEHHMGCANIAIVPPFPI
                     AGIARFFTTNGSTYYSERTGSLVIRDTSVILKYSLKSHVLEHYVQPPGLYIYGLEEDD
                     AGYNLRLYANFKETQKHIPFGSGEFKPGLGPVERGSFPNARFSF"
BASE COUNT         1010 a         1667 c         1632 g         1093 t
ORIGIN      
        1 cgtgatgggc aaggccagct tcggcacgct ggcggacatg agcggacgca tccaggtctt
       61 tgcgcagcag caggcgctgg gcgaagacac ctataacgtg ttcaagacgg cggatgtcgg
      121 cgacatcctc ggtgcggttg gcacggtgat gaagaccaag accggcgagc tgtcgctgcg
      181 cgtgagcgag ctgcgcctgt tggccaaggg cgtgcgcccg ctgccggaga agtgggcggg
      241 gctcaccgac accgagcagc gctaccgcca gcgctatgtc gaccttatcg tgaatcccga
      301 tgtgcgcgcc gtgttcgaga agcgcggcca ggcggtgcgc ttcatccgca gcttcttcga
      361 cgcgctgggc ttcgtcgagg tggaaacgcc gatgctgcag ccgatccccg gcggcgccac
      421 cgcgcgcccg ttcatcacgc atcacaatgc gctggaccgc gacctgtacc tgcgcatagc
      481 gcccgaactc tatttgaagc ggctggtggt cggcggcttc gagcgcgtgt acgagatcaa
      541 ccgcaacttc cgcaatgaag ggctgagcac acggcacaac ccggaattca ccatgctgga
      601 gttctaccag gcgtacgcgg attaccgcga cctgatggac ctgatcgaaa ccctgctgcg
      661 cgatcttgcg atcaccgtcg ccggcagcgg caagctcatc tatcaggaac gcggatacga
      721 cctggagaag cccttcacgc gctggaccat gcgccaggcg gtgaagcact tcaaccaggc
      781 gctggatgaa gcgaagctgg gcgagcgcga ttacctggcg caggccgcgc gcgcgctgcg
      841 catcgaggtg aaggatggct acggcgccgg aaaaatcctc accgagattt tcgagaagac
      901 ggcggaagcc aacctgctcg atccgacctt catcaccgag tacccgaccg aagtctcgcc
      961 gctggcgcgg cagaacgacg ccgatcccac gatcaccgac cgcttcgagc tgttcgtcgg
     1021 cggtcgcgag atcgccaatg gtttctccga gctgaacgat cccgacgacc aggccgcgcg
     1081 tttccgcgcg caggttgcag cgaaagaccg cggcgattcg gaagcgatgc attacgacgc
     1141 cgactacatc cgcgcgctgg agtacggcat gccgccaacc gctggcgcgg gtatcggcat
     1201 cgaccggctg gtgatgttca tgaccgactc gccgtcgatc cgcgacgttc tgctttttcc
     1261 gcagatgcgc ccggaggtat aagcgggatt tcgcggcgcg caggcggtct gatcaccgga
     1321 acatgaagca agccagcctt accgacctgc gcacgcagta cgtgcatcca ccgctgcgct
     1381 ggatgctgat gctggccatg atctcggcga tcggcccgat ggccatcgac atgtacctgc
     1441 cgagcctgcc ggcgctgcag cggcactttc actcggacac cgcggccacg cagctcacgc
     1501 tgtcggcttt cttcatcggc ctggcctgcg ggcagatcat gtacggcccc gtgtccgacc
     1561 gttttggccg caagccgccg ctgctgttcg ggctgggcct gttcacgctg gtgtcgctgg
     1621 cctgcgtgtt cgcgccgtcg atcgaagcgc tgacgctgct gcgtttcgcg caggcgctcg
     1681 gcggcgccgg cggcatggtg atcgtgcgcg ccatcatccg cgaccgctac ccgccgcagg
     1741 acatggcgcg cgtgctgtcg ctgctgctgc tggtgatggg cgtggcgccg atcctggccc
     1801 cgctggccgg cggctgggtc ttccagtggt ggggctggca ggccatcttc atcgtgctca
     1861 ccgcctacgg cgcgctgtgc ttcgccgcgg tggcgtgcgg attgaccgag accatcgtcg
     1921 accgcgcgcc ggccctgcgc atgagcgcga tcgccatcgg ctacgggcgt atgctcgcgc
     1981 accgccgttt tctcggctat gcgctggccg gcggcgtggc ccagggcggc atgttcgcct
     2041 acatctcagc ttcgcccttc gtgttcatcg atttcttcgg gcttagtccg acgcattacg
     2101 gctgggtgtt cggcaccaac gcgctgggcc tgatcagtgc ctcgcagatc aacacgcgcg
     2161 tgctgcgccg tctgcccgcg caacaggtgc tgcggcgtgc catccgcagc tacgcgggtt
     2221 tcggcctgct gctgctgctc tgcgcctggc gcggctgggg tggcgtgtgg ggcgtgttga
     2281 ttccgctggt ctgctgcatt tcctcgctgg gtttcacctt tcccaactcc acggccgctg
     2341 cgatggcgcc gttcggcgac cgcgccggca gcgcatcggc tctgctcggc acgctgcagt
     2401 tcgccatcgc ggcatgcacc ggcgcgctgg tcggccatct gctgcgcaac agcgccgtgc
     2461 cgatggcggc cgtcatcgcg ggctgcggct gcacctcggc ggcgctgctg gcctgggtgc
     2521 gtccacacga gtcgcacaag ccagttcgcg gtgattcctg aggccaccgc agtgccggta
     2581 ttgatgttat taaatattta ttataaataa ttatatatta tattttataa catcaagagc
     2641 cggtgtgcgg ttgggccaaa aaaacgcctg cggcgcggtc gcatcgaatc acccggtgcc
     2701 ggaatcctgg gtgccgaggt tgaacaccag gccgaacaag tcttccagat agcagcgcgg
     2761 caccgccgca tcttcctcaa ccacgacgca gcccgccgcc agcagccccg ccctggttcc
     2821 ctggacgtcg gcgcagagca gatcgaacac cggtgcatgc gcgctgcctc cttccacgta
     2881 gagcacgaag gcgccggtat taaatcccgc cagtgcgtcg ctgcgatgaa ccagcctgct
     2941 cgtggaaatg cacggcttct gccgagcgac cggtgcgcag gatgacgtcg cgcgatgacc
     3001 ggatgggggc gggtgcagcg cggcgtagtc acccacggac ggttgatccc cggaggctga
     3061 tggcggacat gctgccggtg gccgcattcg agctcggcga tccagtcgcc tgcgtcatcc
     3121 tggtggaatc cagtgatgcg cttttgcatg cgtgacgagg ttcggcgccg tagcgatggg
     3181 ccgggcccgc cgatctattg gccggccggc ggttgccaga gttccacctt gttgccctcg
     3241 gggtccatca cccagccgaa gatgccgaac tccgattcat ccacctggtc cagtacctgg
     3301 cagccttcct cgcgcagtga tttgagcagc ccacgcagat cggccacgcg gtaattgacc
     3361 atgaaggtct tgcccggggc gaagcgctgc ccatcaccga tggaccaagc cgtcgttcca
     3421 gcgacgggag ccccttggtc gtcaatccag tcgaatgccg cgccacccca ggcctgtacg
     3481 tcgatgccaa gatgggtctt gtaccaggcg cagagcttcg ccggatcctt tgcactgaaa
     3541 aagatgccgc caattccggt cacacgtttc atgaatcacc atccgatgcc aggagcagaa
     3601 gcgctcgcgg ccgagcgtac tctcaaaccg gttgccgatc ggtatgccac cggccgcatt
     3661 tatcgatttc tgccgatgag acagaaagca tgaccgaatg gatcgctgca gaaggccacg
     3721 cagccatgcc catcattttc actttcctga acccgcacgt tgctttatag aagcgaacgc
     3781 cctccgccag gtcgggcaca tcgatgctgc cttgttaggc gacggtatca ctggactgag
     3841 aatgggggaa cgaatgcgtt atgcaagctt cccactcgtt gtcctttgac cataacgaag
     3901 tgtccaggcg aggcgacgca ttgactattg gccgtcagag cggactgagc tgttacagga
     3961 acgggtgatt ggcgagtggg aacaaaaacc gtttcagtct ttccagctac gaaccccggc
     4021 aggattaata ggttggccca ttcattttca gtttctgtgt catagccaag atagggagtg
     4081 ccagagcagt ccgatgattc aaagactatt ggttggggag gaccattgaa tccgcggcgg
     4141 ctaacgatca ctccaaacgt gtgattgttt agagtcatca agacagctgc attcccatcc
     4201 gcgtcgtgcg ggtaaatatt gtcggcgata gagacgattg gcccgacaat ggttccattt
     4261 gaatctacaa caataggcgt tccctcagca tgagctgacg ccagtgtgaa taccaatccg
     4321 aagattgcaa aaaaacgata ggtgcgaacc atatgtacct cctcactaaa tcgataaccc
     4381 tcgcagaggc tgtcgcctaa cacccaagct caggggcggc gccaaagctg cggctgtttc
     4441 tttcaaatcg cagctctggt gacgtcccct ggagcgcctt gttaggcgac gctctcaccg
     4501 aggcttcctc tgtgacgccg ctctgatatt gcgcctgagc gtctgcaaga tcacgaatgc
     4561 ttcgtcctcg gctgtagttt cagcaaggta ttgccaagga tacaagctgt gccatggata
     4621 tgttccgcgg ccagtcggat cgtaggaaat aaccgcagcc tttgacttga gccgggcagc
     4681 gagcccctcg actttcgccg tagccaaaag atcgcgtaat ttctgtgacg agcgcttcgc
     4741 tcttccctgg gcaacgcgca gcgcggaatt gagaagagct tcgtactcag cagcatgacg
     4801 gcgaagcgta gcaagattgt cacgcttggt gattttgcga aatccactta tggtgtcgcc
     4861 taacacccaa gctcagccgc ggcccgtaaa ttgcgcttct tcttttcgca atttatggga
     4921 cgtcggctgg agcgcattgt taggcatatg tgccccttaa cttaaaagct gaatcttgca
     4981 tttggaaatg aaccgcgctc cactggcccc aaccccggct tgaactctcc agacccgaaa
     5041 ggaatgtgtt tctgcgtttc cttgaaattg gcgtataggc gcaggttgta accagcgtcg
     5101 tcttcctcca ggccatagat gtacaacccc ggtggctgta catagtgctc tagaacgtgc
     5161 gacttcaagc tgtacttcaa gatgacgctg gtatcgcgaa taaccaaact gcctgttcgt
     5221 tctgagtaat aggtgctgcc attcgttgtg aaaaagcgag caatgccggc aataggaaag
     5281 ggaggcacga tggcgatatt ggcgcacccc atatggtgct ctccatacca ctcgatacgg
     5341 accgtgcccg ctttgtttgg caaagctatg gtctcgacct gctctggctt catatgccta
     5401 ac
//
LOCUS       DASZZC010000761         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1281839,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000761 DASZZC010000000
VERSION     DASZZC010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281839"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VHE37_16330"
     CDS             complement(<1..1294)
                     /locus_tag="VHE37_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVT37155.1"
                     /translation="MLPAWLLFAVSIAYVGLLFAIAHAGDRAAARQPAPAARPWVYSL
                     ALAVYCTSWTFYGAVGRAATSGWDFLPINLGPVLMFVAGYPLVLRIVEISKRNNITSI
                     ADFIGARYGRHQSVAMLVTLIAVTGVLPYIALQIKAVAFSFETLAGPGTAPARDSALV
                     IAGLLAVFAILFGTRQVVSSETHHGMMLAIAFESVIKLVTFLAVGLYAVYGVYHGFAD
                     AYAHALVLPQVLHAPESGDWQAGFFTQTVLAMGAVLCLPRQFQVAVVENTAAADVRAA
                     RWIFPLYLGLLSVLVLPLAAAGLQRLPEARADTYLLALPLSQGHPLLALAAYVGGFSA
                     ATSMVIVACIALSTMLCNEVVMPVLLRMRGAGYARGRDLSGLLKRIRRIAIVAIVALA
                     YLYYRWFGGPGTLSSIGLLSFSAVAQFLPALIGGLYWRR"
     gene            1416..1736
                     /locus_tag="VHE37_16335"
     CDS             1416..1736
                     /locus_tag="VHE37_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HVT37156.1"
                     /translation="MPQAASAAPPSTERIMALPAFIQLRNARRALALKLTVAMLAIYY
                     GFVLMVAFAPHTLAEPVSGHISLGILLGLGVILAAVVLTGIYVRRANGEFDRLTQDIL
                     RSAS"
     gene            1733..3391
                     /locus_tag="VHE37_16340"
     CDS             1733..3391
                     /locus_tag="VHE37_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HVT37157.1"
                     /translation="MKRAQTIIAVVAGMLPAWAQAAAIEGQTVKQETNWTAIIMFFAF
                     VAATLGITWWAAQRTRSRAHFYAAGGGITGFQNGLAIAGDYMSAASFLGISALVFGSG
                     FDGLLYSIGFLVGWPIIMFLVAERLRNLGKFTFADVASYRLEQTPIRVLAASGTLVTV
                     AFYLIAQMVGAGQLIKLLFGLDYNYAVIIVGVLMMIYVLFGGMLATTWVQIIKAVLLL
                     GGASFIALMVMSSVGFSFEGLFAKAVDVHKDHAAILGPGKLVKDPVSAISLGLALMFG
                     TAGLPHILMRFFTVKDAKEARKSVFFATGFIGYFYILTFIIGFGAIVLVGTDPQYLDD
                     KGALRGGNNMAAVHLAHAVGGNLFLGFISAVAFATILAVVAGLTLSGASAVSHDLYAS
                     VYARGRTDEAHELRVSRIATVALALLAIVLGIAFEKQNVAFMVGLAFAVAASANFPVL
                     LLSMFWSRLTTRGAVIGGALGLVTAVTLCVLGPAVWVKVLGNKEAIFPYDAPGLFSMT
                     VAFLGCWLGSITDSSARAEQERGRYLAQFVRAETGLGASGASDH"
     gene            3460..>5362
                     /gene="acs"
                     /locus_tag="VHE37_16345"
     CDS             3460..>5362
                     /gene="acs"
                     /locus_tag="VHE37_16345"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HVT37158.1"
                     /translation="MSDNNIQSVLEEKRVFPPSAETVARARVTPEKLRQLRAAAEADP
                     VAFWAELAKKELGWQTPFRKTLDDSRAPNYAWFADGKLNVAANCVDRHLGARGDKIAI
                     RFEGEKGDTRNLTYRQLHAEVCRLANGLASLGVHKGDRVVIYLPMTPEAVIAMLACAR
                     IGATHSVVFGGFSAHSLKDRIEDATAKVLITADGGHRAGNIVELKKAADEALSGGCPS
                     IEKVVVCKRTGHAVHMQQGRDLWWSELTAGQPERCDPVWVEAEHPLFLLYTSGSTGKP
                     KGIQHSSGGYLLGAHMTTQWVFDLRDDDVFWCTADVGWVTGHTYVCYGPLSNGATIVM
                     YEGAPTIPDAGRFWKICQDHGVTIFYTAPTAIRALMKLGESFPAKYDLSKLRLLGSVG
                     EPINPEAWIWYHRVIGQEKLPIVDTWWQTETGAIMMSPLPGATPTKPGSCTQPLPGIF
                     VDLVSESGEVIRQQDAGGLLVVTKPWPSMLRTIWNDNERYLKTYFPQFDNQAVAAGGG
                     ARQWLYVAGDTTHRDADGYYWIMGRADDVLKVSGHRLGTMEVESALVSHPRVAEAAVV
                     GRPHEIKGESIFAFVVCKGERPQGADAEKLLKDLREHVGKELGALAKPDDIRFADNLP
                     KTRSGKIMRR"
BASE COUNT          931 a         1796 c         1795 g          840 t
ORIGIN      
        1 cgcggcgcca gtacaggccg ccgatcagcg ccggcaggaa ctgggcgacc gccgagaacg
       61 acagcaggcc aatcgacgac agcgtgccgg gtccgccgaa ccagcggtag tacaggtaag
      121 ccagcgcgac gatggcgacg atggcgatgc ggcggatgcg cttgagcaga cccgacaggt
      181 cgcgaccgcg cgcatagccg gcgccgcgca tgcgcagcag caccggcatc accacctcgt
      241 tgcacagcat ggtcgacagc gcgatgcagg cgacgatcac catgctggtg gccgcagaaa
      301 atccgccgac gtaggccgcc agcgccagca gcggatggcc ttgcgacaac ggcaatgcga
      361 gcaggtaggt gtcggcccgg gcttcgggca ggcgctgcag cccggccgcc gccagcggca
      421 gcaccagcac gctcagcagc cccagataca gcgggaagat ccagcgcgcg gcacgcacgt
      481 ccgccgctgc ggtgttctcc accaccgcga cctggaactg gcgtggcagg cacagcacag
      541 cgcccatggc cagcacggtc tgcgtgaaga agccggcctg ccagtcgccg gactccggcg
      601 catgcagcac ctgcggcaga accagcgcgt gggcgtaggc atcggcgaag ccgtggtaca
      661 cgccatagac cgcgtacagg cccaccgcca ggaaggtgac cagcttgatc accgattcaa
      721 acgcgatggc cagcatcatg ccgtgatggg tttcgctcga caccacctgc cgcgtgccga
      781 acaggatggc aaagaccgcg agcaggcccg caatgaccag tgcactgtcg cgcgcaggcg
      841 cggtgccggg ccccgccagc gtttcaaaac tgaaagccac cgccttgatc tgcagcgcga
      901 tgtacggcag cacgccggtg acggcgatca gcgtgaccag catggccacg ctctggtggc
      961 ggccatagcg cgcaccgatg aaatcggcga tggacgtgat gttgttgcgc ttgctgatct
     1021 cgacgatgcg cagtaccagc gggtagccgg ccacgaacat cagcaccggc cccaggttga
     1081 tcggcaggaa atcccagccg ctggtggcgg cacggcccac ggcgccgtaa aacgtccacg
     1141 aagtgcaata gaccgccagc gcgaggctgt acacccaggg gcgcgcggcc ggagccggct
     1201 ggcgcgcggc cgcgcggtca ccggcatggg cgatggcgaa cagcagcccg acataggcga
     1261 tcgacaccgc gaacaggagc caggcgggca gcatgcgggc agcttagctc agcagggctg
     1321 cgtgccaggc cgcatgcgac taaggtccaa ttccggcgcg cgggcggcgc ggctaaagtg
     1381 cagccgtccc gaatccctgc gtgaggaaca tgtccatgcc gcaagccgca tccgccgcgc
     1441 cgccgagcac cgagcgcatc atggcgctgc ccgcgttcat ccagctgcgc aatgcacgcc
     1501 gcgcactcgc cctcaagctc accgtcgcca tgctggcgat ctattacggt ttcgtgctga
     1561 tggtggcgtt tgcgccgcac acgctggccg aaccggtgag cggacacatc tcgctgggca
     1621 tcctgctcgg actgggcgtg atcctcgccg cggtggtgct gaccgggatc tacgtgcgcc
     1681 gggccaatgg tgaattcgac cggctgaccc aggacatcct gcggagcgca tcatgaaacg
     1741 cgcgcagacc atcatcgcgg tggtggccgg aatgctgccg gcctgggcgc aggccgcggc
     1801 gatcgagggc cagacagtga agcaggaaac caactggacc gcgatcatca tgttcttcgc
     1861 cttcgtcgcg gccacgctgg gcatcacctg gtgggcagcg cagcgcacgc gcagccgcgc
     1921 gcatttctac gcggccggcg gcggcatcac cggtttccag aacgggctgg ccatcgccgg
     1981 cgattacatg tcggcggctt cgttcctcgg catctcggcg ctggtgttcg gctcgggctt
     2041 cgacggcctg ctttactcga tcggcttcct ggtcggctgg ccgatcatca tgttcctggt
     2101 ggccgagcgg ctgcgcaacc tgggcaagtt caccttcgcc gatgtcgcct cctaccgtct
     2161 ggagcagacg ccgatccgcg tactcgccgc ctccggcacg ctggtgacag tggccttcta
     2221 cctcatcgca cagatggtgg gcgcgggcca attgatcaag ctgctgttcg gcctcgatta
     2281 caactacgcg gtgatcatcg tcggcgtgct gatgatgatc tacgtgctgt tcggcggcat
     2341 gctggccacc acctgggtgc agatcatcaa ggcggtgctg ctgctcggcg gggccagctt
     2401 catcgcgctg atggtgatgt ccagtgtcgg attttccttc gaagggctgt tcgccaaggc
     2461 ggtggacgtg cacaaggacc acgccgccat cctcggcccg ggcaagctgg tgaaggaccc
     2521 ggtctcggcc atttcgctgg gactggcgct gatgttcggc actgccggcc tgccgcatat
     2581 cctgatgcgc ttttttaccg tgaaggacgc caaggaagcg cgcaagagcg tgttcttcgc
     2641 cacaggcttc atcggctact tctacatcct gaccttcatc atcggcttcg gcgccatcgt
     2701 gctggtcggc accgacccgc agtatctcga cgacaagggc gcgctgcgcg gcggcaacaa
     2761 catggcggcg gtgcacctgg cccacgcggt cggcggcaac ctgttcctgg gcttcatctc
     2821 ggcggtggcc ttcgccacca tcctcgcagt ggtcgcgggc ctgacgctgt ccggagccag
     2881 cgcggtgtcg cacgatctct acgccagcgt gtacgcccgc ggccgcacgg acgaagcaca
     2941 tgaattgcgc gtttcgcgca tcgccacggt ggcgctggcg ctgctggcca tcgtgctggg
     3001 catcgccttc gagaaacaga atgtcgcctt catggtcggg ctggcgttcg ccgtcgcagc
     3061 ctcggccaac tttccggtgc tgctgctgtc gatgttctgg agccggctga ccacgcgcgg
     3121 tgcggtcatc ggcggggcgc tgggcctggt cacggcggtg acgctgtgcg tgctgggacc
     3181 ggccgtctgg gtgaaggtgc tcggcaacaa ggaggcgatc ttcccgtacg acgcgcccgg
     3241 cctgttctcg atgacggtgg cgttcctcgg ctgctggctg gggtcgatca ccgactcgtc
     3301 ggcgcgcgcc gagcaggagc gtggccgtta ccttgcgcag ttcgtgcgcg ccgaaacggg
     3361 gctcggcgcc agcggcgcca gcgaccactg agcgccgtgt tgcccgcaat gtttcccgat
     3421 atttcctaag atcgactccc gcatccgccg gagactgcca tgagcgacaa caacatccag
     3481 tccgtgctcg aagaaaagcg cgtgtttccg cccagcgcgg aaaccgtggc gcgcgcgcgg
     3541 gtcacccccg agaaactcag gcagctgcgc gccgcggcgg aagccgatcc ggtcgcattc
     3601 tgggcggagc tggccaagaa ggaacttggc tggcagacgc ccttccgcaa gacgctcgac
     3661 gacagccgcg cgccgaacta cgcctggttt gccgacggca agctcaacgt tgccgccaac
     3721 tgcgtcgacc gtcatctggg cgcgcgcggc gacaagatcg ccatccgctt cgaaggcgag
     3781 aagggcgaca cgcgcaatct cacctacagg cagctgcacg ccgaggtgtg ccggctggcg
     3841 aacgggctgg catcgctggg cgtgcacaag ggcgaccgcg tggtgatcta cctgccgatg
     3901 acgccggaag cggtgatcgc catgctggcc tgcgcgcgca tcggagccac gcacagcgtg
     3961 gtgttcggcg gcttttccgc gcactctctg aaggaccgca tcgaagatgc cacggcgaaa
     4021 gtcctgatca ccgccgatgg cggccatcgc gccggcaaca tcgtggagct gaagaaggcc
     4081 gcggacgagg ccctgtccgg cggctgcccg agcatcgaga aggtggttgt gtgcaaacgc
     4141 accggccacg cggtgcacat gcagcagggc cgcgacctgt ggtggagcga gctcaccgcg
     4201 ggccagcccg agcgctgcga cccggtgtgg gtggaagccg agcacccgtt gttcctgctc
     4261 tacacctctg gctccaccgg caagcccaag ggcatccagc attcctccgg cggttacctg
     4321 ctgggcgcgc acatgaccac gcagtgggtg ttcgacctgc gcgacgacga tgtgttctgg
     4381 tgcaccgccg acgtcggctg ggtcaccggc cacacctatg tgtgctacgg cccgttgtcc
     4441 aacggcgcca ccatcgtcat gtacgagggc gccccgacca ttcccgacgc cggccgcttc
     4501 tggaaaatct gccaggatca tggcgtcacc atcttctaca ccgcgcccac cgcgatccgc
     4561 gcgctgatga agctgggcga atcgttcccg gcgaagtacg acctgtcgaa gctgcgcctg
     4621 ctcggcagcg tcggcgagcc gatcaacccg gaagcctgga tctggtacca ccgtgtgatc
     4681 ggccaggaaa agctgccgat cgtcgacacc tggtggcaga cggaaacggg cgccatcatg
     4741 atgtcgccgc tgcccggcgc cacaccgacc aagccgggct cctgcacgca gccgctgccg
     4801 ggtatcttcg tcgacctggt gagcgaatcc ggcgaagtga tccggcagca ggacgccggc
     4861 ggcctgctgg tggtgaccaa gccgtggccg tcgatgctgc gcaccatctg gaacgacaac
     4921 gagcgctacc tcaagaccta cttcccgcag ttcgacaacc aggctgtcgc cgcaggcggc
     4981 ggcgccaggc agtggcttta cgtggccggc gacaccacgc accgcgatgc cgatggctac
     5041 tactggatca tgggccgcgc cgatgacgtg ctgaaggtgt cggggcaccg cctgggcacc
     5101 atggaagtgg aaagcgcact ggtcagccac ccgcgcgtgg ccgaggcggc ggtggtcgga
     5161 cgtccgcacg agatcaaggg cgaaagcatc tttgccttcg tggtgtgcaa gggcgaacgg
     5221 ccacagggcg ccgacgccga aaagctgctg aaggatctgc gtgagcacgt gggcaaggaa
     5281 ctgggcgcgc tggccaagcc cgacgacatc cgcttcgccg acaacctgcc gaagacgcgc
     5341 agcggcaaga tcatgcgccg cc
//
LOCUS       DASZZC010000762         5115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_834158,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000762 DASZZC010000000
VERSION     DASZZC010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834158"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VHE37_16350"
     CDS             <1..843
                     /locus_tag="VHE37_16350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVT37159.1"
                     /translation="ALLLGAVMIGFAPIFVRLTDVGYTACAFWRAALALPVLAIVARP
                     RVHQLQWNSLRWLLLAGLFFGADLAVWHQSIRLTSVANATLLANFAPVFVTGAAVLVL
                     HERVRASFVGGLVLALAGAATLVGNSALQGGGNVLGDLCGIGASVFYAGYLIVVARAR
                     SRYRAAEVMLWTSLATAALLLLIASVEHETMWPQSARGWGVVLGLALLSQVLGQGLIA
                     YALAHLSAAFSAAGLLVQPVAAALLAWLLLGEPFGWRQGIGGVIVLAGIVLCRLSMPT
                     APPE"
     gene            910..2073
                     /locus_tag="VHE37_16355"
     CDS             910..2073
                     /locus_tag="VHE37_16355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT37160.1"
                     /translation="MSRKNTRLHGLDTLRAAAIVLVFMYHYMVFVSGEPTFGWGSTVG
                     WAGVDLFFVLSGYLIADQLFAGLAARRELSLRAFYARRLLRTLPDYYVVLALYFLFPA
                     AMGGKTPPPLWQFLSFTQNILLHPGTAFSHAWSLCIEEQFYLLLPLALVIGARWRGSV
                     RHAWLALIGLMLAAIALRSMLWLQYAAADERSSDWYYRYIYYASWCRCDEFLPGVALA
                     LIKHFHAPLWMRVLGWGNRLLAAGALAVALMFYLGATRYYVDGAGYPYFMTGFGYTLL
                     AASFALLLLAALAPGSLLQRVRVPGAAPLAAWSYAIYLSHKPLAHVLHQPLHQLGIAG
                     FNEVLVIAAACVLAGWLLHRCVETPFMRWRARRWPDNFPAAAPAAAPALQPGM"
     gene            complement(2116..2553)
                     /locus_tag="VHE37_16360"
     CDS             complement(2116..2553)
                     /locus_tag="VHE37_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37161.1"
                     /translation="MQKHLAALSCAVLFSAAAVAAPADEKPFKEGPVTDVSYIRVKDG
                     KFFDYMAYLDSTWKTEQEALKKAGLILDYRVYGATPHSPHDANLVLTITYPNYAALDR
                     TAEFEAVTAKVEGSLKASDKAFGDRDSIREILGDELVQELMLK"
     gene            complement(2623..3387)
                     /locus_tag="VHE37_16365"
     CDS             complement(2623..3387)
                     /locus_tag="VHE37_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37162.1"
                     /translation="MRPWWLPSLLLPLCAAAAVPVQLSIDNQAKLPEAQVQAMRDEYA
                     AWAQRVYAYLHVNAPAPVHIALTRDAHVGMYVDDTLYMPPDEHDEMLETFIHELAHQA
                     TGHESSFFFKEGIATHTLEALYAQDGKIPQGWPQYGEPCDAWVALFAARHQLLSLREA
                     LYWAHFDGHDADGDYRSWQIYLMGASFARWFIGAYGYDAYKKMFDTQQLPAAAEKLEH
                     RWLAAVAAEKFAPFDPAHELPRGERYQGYARRLRGG"
     gene            complement(3365..>5115)
                     /locus_tag="VHE37_16370"
     CDS             complement(3365..>5115)
                     /locus_tag="VHE37_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT37163.1"
                     /translation="MGGIIGRLLHEFAVTIIVAVLVSGMVSLTLTPLLCSRWLHRDQG
                     RHGRMYQMFEHAFDAWRDAYDRSLRWVLDHQRFTLLLFFGSIVLTVVLFRSASYGLLP
                     NDDTGQLIGSTEGAQDISFDAMAQKQQQAANLIRQSPDVDATMAFIGSGSSSGLNQGR
                     ILVRLKPPSERPSADTVLQHLRPKLASIPGFKVFLQNIPVIRIGGQSTKAQYQYTLQD
                     ADLEELYQWAPKLADKLSTMDGFLDVNTDQQLRLPKVIVHIDRAHAAALGVTPDEIEN
                     ALYSAYGARQVSSIYTPSNQYAVIMELLPQFQMDPQALSLLYVQGTGSTLVPLSAVAS
                     FERSVGPLSVSHLGQLPSVTVSFNLKPGLALGDAVKSVNRAQEELKMPDTLLGSFQGA
                     AQAFQSSLGNMGLLLTAAILVIYIVLGILYESFVHPITILSGLPSAGMGAVATLMLFG
                     LDVNLYAFVGIIMLIGIVKKNAIMMIDFALEAQRHQNMAPHEAIYQACIIRFRPIMMT
                     TMAALFGTLPIALGIGAGGDSRRPLGLCVVGGLLVSQMLTLYLTPVIYLYLDRLGRRL
                     SRRRGEPPADAPVVAA"
BASE COUNT          758 a         1666 c         1778 g          913 t
ORIGIN      
        1 gccctgctgc tcggcgcggt gatgatcgga tttgcgccga tcttcgtgcg cctgaccgat
       61 gtcggttaca ccgcctgcgc gttctggcgc gcagcgctgg cgctgccggt gctggcgatc
      121 gtcgccaggc cgcgcgtgca ccagttgcaa tggaactcgc tgcgctggct gctgctcgcc
      181 gggcttttct tcggcgccga tctcgcggtg tggcaccagt cgatccgcct gacctcggtg
      241 gccaacgcca cgctgctcgc caacttcgca ccggtgttcg tcaccggcgc cgcggtgctg
      301 gtgctgcacg agcgcgtgcg cgcgagcttc gtcggcgggc tggtgctggc gctcgccggc
      361 gcggccacgc tggtgggcaa cagcgcgctg cagggcggcg gcaacgtgct gggcgacctg
      421 tgcggcatcg gcgcgtcggt gttctacgcc ggctacctga tcgtggtggc gcgcgcgcgc
      481 agccgctacc gcgcggcgga agtgatgctg tggacttcgc tggccactgc cgcgctgctg
      541 ctgctcatcg ccagcgtcga gcacgagacg atgtggccgc aaagcgcgcg cggctggggc
      601 gtggtgctgg ggctcgcgct gctgtcgcag gtgctgggcc agggcctgat cgcgtatgcg
      661 ctggcgcacc tgtcggcggc gttttccgcg gcaggactgc tggtgcagcc ggtggcggcg
      721 gcgctgctgg cgtggctgct gctgggcgaa ccgttcggct ggcgccaggg catcggcggc
      781 gtcatcgtgc tcgccggcat cgtgctgtgc cggctttcca tgccgaccgc accgccggaa
      841 tagatgttat aatttatagt tcaattaaaa aataaaacaa caattaataa caacgaaaat
      901 ggcatcgcgg tgagcaggaa aaacacgcgt ctgcacgggc tcgacacgct gcgcgcggca
      961 gccatcgtgc tggtgttcat gtaccactac atggtgttcg tcagcggcga gccgaccttc
     1021 ggctggggca gcaccgtggg ctgggccggt gtcgacctgt tcttcgtgct gtcgggttat
     1081 ctcatcgccg accagctgtt cgccggcctc gccgcgcggc gcgagctttc gctgcgcgcg
     1141 ttctacgcgc gccggctgct gcgcacgctg ccggactact acgtggtgct cgcgctgtac
     1201 ttcctgtttc ccgcggccat gggcggcaag acgccgccgc cgctgtggca gttcctcagc
     1261 ttcacccaga atatcctgct gcacccgggc acggcgttct cgcacgcgtg gtcgctgtgc
     1321 atcgaggagc agttctacct gctgctgccg ctggcgctgg tcatcggcgc gcgctggcgc
     1381 ggatcggtgc gacatgcgtg gctggcgctg atcggcctga tgctcgccgc catcgcgctg
     1441 cgcagcatgc tgtggctgca gtacgccgcg gcggacgagc gcagcagcga ctggtactac
     1501 cgctacatct attacgcgtc atggtgccgc tgcgacgagt tcctgcccgg cgtcgcgctc
     1561 gcgctgatca agcatttcca cgcgccgctg tggatgcgcg tgctcggctg gggcaaccgg
     1621 ctgctcgcgg ccggcgcgct cgcggtggcg ctgatgttct acctgggcgc gactcgctac
     1681 tacgtcgatg gcgccggtta tccatacttc atgaccggtt tcggctacac gctgctggcc
     1741 gcgagtttcg cgctgctgct gctcgcggca ctggcgccgg gttctctgct gcagcgggtg
     1801 cgcgtgccgg gtgctgcccc gctggcggcg tggtcctacg cgatctatct ctcgcacaag
     1861 ccgctggcgc atgtgctgca ccagccgctg catcagctgg gcattgcggg attcaatgaa
     1921 gtgctggtga tcgcggcggc ctgcgtgctt gcgggctggc tgctccatcg ctgcgtggaa
     1981 acgccgttca tgcgctggcg cgcgcggcgc tggccggaca atttcccggc ggctgccccc
     2041 gctgcggcgc cggcgctgca gccgggcatg tgaaaacgcg ggaaggccgc ggcgtccggc
     2101 cttcccgcga gcgcatcact tcagcatcag ctcctgcacc agctcgtcgc cgaggatctc
     2161 gcggatcgag tcgcggtcgc cgaaggcctt gtcggaggcc ttcaggctgc cttccacctt
     2221 ggccgttacc gcctcgaact ccgccgtgcg atccagcgcg gcgtaattgg gataggtgat
     2281 ggtcagcacc aggttggcgt cgtgcggact gtgcggcgtc gcgccgtaga cgcggtaatc
     2341 gaggatcagg ccggctttct tcagcgcctc ctgctcggtc ttccaggtgc tgtccaggta
     2401 cgccatgtag tcgaagaact tgccgtcctt gacgcggatg taggagacgt cggtcaccgg
     2461 cccttccttg aacggcttct cgtcggcggg tgcggcgacg gcggcggcgc tgaacaggac
     2521 cgcgcaggac aatgcagcaa gatgcttttg catgacgtag ctcccatgaa gtggaaacgc
     2581 gcatgtcggc cggagatggc cggcagcgga gtgtagtccg cttcagccgc cccgcaagcg
     2641 ccgcgcgtag ccctggtagc gctcgccgcg cggcagttca tgcgccggat cgaacggcgc
     2701 gaatttctcc gcggccacgg cggcgagcca gcggtgttcg agtttctccg ccgccgcggg
     2761 cagctgctgc gtgtcgaaca tcttcttgta ggcgtcgtag ccataggcgc cgatgaacca
     2821 gcgtgcgaac gatgcgccca tcaggtagat ctgccaggag cggtagtcgc cgtcggcgtc
     2881 gtggccgtcg aaatgcgccc agtaaagcgc ctcgcgcagg ctgagcagct ggtggcgcgc
     2941 ggcgaacagc gcgacccacg cgtcgcacgg ctcgccgtac tgcggccagc cctgcgggat
     3001 ttttccgtcc tgcgcgtaca gcgcttccag cgtgtgcgtg gcgatgcctt ccttgaagaa
     3061 gaagctcgac tcgtggccgg tggcctgatg agccaattcg tgaatgaagg tctcgagcat
     3121 ctcgtcgtgc tcgtccggcg gcatgtacag ggtgtcgtcc acgtacatgc ccacgtgcgc
     3181 gtcgcgcgtc agcgcgatgt gcaccggcgc cggcgcgttc acgtgcaggt aagcgtaaac
     3241 gcgttgcgcc cacgccgcgt actcgtcgcg catggcctgc acctgcgcct ccggcagctt
     3301 ggcctggttg tcgatcgaca gctgcaccgg caccgccgcg gcggcgcaca gcggcagcag
     3361 caggctaggc agccaccacg ggcgcatcgg cgggcggctc gccgcggcgc cgcgacaggc
     3421 gccggccgag acggtcgagg tagaggtaga tcactggcgt caggtacagc gtgagcatct
     3481 gcgacaccag caggccgccg accacgcaca ggcccagcgg ccggcgcgaa tcgccgccgg
     3541 cgccgatgcc cagcgcgatg ggcagcgtgc cgaacagcgc ggccatggtg gtcatcatga
     3601 tcgggcggaa gcggatgatg caggcctggt agatggcctc gtgcggcgcc atgttctggt
     3661 ggcgctgcgc ttccagcgcg aagtcgatca tcatgatcgc gttcttcttg acgatgccga
     3721 tcagcatgat gatgccgacg aaggcgtaga ggttcacgtc gagcccgaac agcatcagcg
     3781 tggccaccgc gcccatgccc gcgctcggca ggccggacag gatggtgatc gggtgcacga
     3841 acgattcgta caggatgccg agcacgatgt agatgaccag gatggccgcc gtcagcagca
     3901 ggcccatgtt gccgagcgag gactggaacg cctgcgccgc gccctggaag ctgccgagca
     3961 gcgtgtcggg catcttcagc tcttcctgcg cgcggttcac cgacttcact gcgtcgccca
     4021 gcgccaggcc gggcttgagg ttgaacgaca ccgtcaccga cggcagctgc ccgaggtgcg
     4081 acaccgacag cgggcccacg ctgcgctcga agctggcgac cgccgacagc ggcaccagcg
     4141 tagagcctgt gccctgcaca tagagcagcg acagcgcctg cgggtccatc tggaattgcg
     4201 gcagcaactc catgatcacc gcgtactggt tgctcggcgt gtagatcgac gacacctggc
     4261 gcgcgccgta ggccgagtac agcgcgttct cgatctcgtc cggcgtcacg cccagcgcag
     4321 cggcatgcgc gcggtcgata tgcacgatga ccttgggcag gcgcagctgc tggtcggtgt
     4381 tgacgtcgag gaagccgtcc atggtcgaca gcttgtcggc cagcttcggc gcccactggt
     4441 acagctcctc gaggtcggcg tcctgcagcg tgtactggta ctgcgccttg gtcgactggc
     4501 caccgatgcg aatcaccggg atgttctgca ggaacacctt gaagccggga atcgatgcca
     4561 gcttcggccg caggtgctgc agcacggtgt cggcagaggg ccgctcgctg ggcggcttca
     4621 gtcgcaccag gatgcggccc tggttgaggc ccgacgacga gccgctgccg atgaaagcca
     4681 tggtggcgtc gacatcggga ctttgccgga tcaggttggc cgcctgctgc tgcttctgcg
     4741 ccatggcgtc gaagctgatg tcctgcgcgc cctcggtcga gccgatcagc tggccggtgt
     4801 cgtcattggg cagcagtcca taagaggcgc tcctgaacag caccacggtc agcacgatgg
     4861 aaccgaagaa cagcagcagg gtgaagcgct ggtgatcgag cacccagcgc agcgagcgat
     4921 cgtaggcgtc gcgccaggcg tcgaaggcat gctcgaacat ctggtacatg cgtccgtggc
     4981 gtccctggtc gcgatgcagc cagcgcgagc acagcagcgg cgtgagcgtg agcgagacca
     5041 tgcccgacac cagcacggcg acgatgatgg tgacggcaaa ctcgtgcagc aggcggccga
     5101 tgatgccgcc catga
//
LOCUS       DASZZC010000763         6078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_883921,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000763 DASZZC010000000
VERSION     DASZZC010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883921"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2706)
                     /locus_tag="VHE37_16375"
     CDS             complement(<1..2706)
                     /locus_tag="VHE37_16375"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HVT37164.1"
                     /translation="MRVFLATVLLVPLSAAANPSGVGATSGLAATNGITGLGTANVQV
                     NQAATHAVINWNDFSIDSGELVQFNQPSATAAILNRVTGGNLSQIYGQMKANGRVLLV
                     NPNGIVFGSGGQVDVGGLVASTLNITDANFNTDHWQFDGVADGNVPGRVVNDATINAA
                     RGGFVALLGQGGVQNDTAISADHGSILLAAGSSIFVDPVGDGLMLYQVTAPTLDHYHY
                     STDADPNQNDAGNDGVLNHGTLTADGGRIALETYNDFVTYAGLGNTGGLIRAQTASTN
                     EQGQVILVARGSGVLQAGELDASGPGNGSVSIRTDQAAALRPPQDYGYTGDGNNPPSV
                     IAHSFSIDVADFSPQRVTVAADVIDLRSSGDFNFGGVTVKGQGGANPASVRLQGNQLE
                     LSGVTLSSPALTLVASGTPSDAAPDAGRVTVTGSTLSGSTLVVQGANLDLRSTMDYAT
                     SIDLTGSTRITLEGATIGSSTPGAVSFTTPREAIVDNSTLNATTLTTQVGTDHVQFDD
                     GFNVHNSTLTATTMQLNADGVNVDGASALTTAGLMDIHAGVDARGVPLHAGSLHISGG
                     AFLEDDDVRTTAGMQFDSPDLQVRGTTLSGTTLAVNGSGTPSEFDLTPSATTGNAASA
                     TFTDSIIVNTGVVVIGDTAVHAPQISFTTHGGSATGLSDLFISGSKLDGSTLQVQATG
                     IDIRGSNANAQSTLDFGTSLDLASSGRTAIQDTLIGGQAIAPVRITAGENLSLQSTTI
                     HARDLTLRAAYGTADPFNGIFLTDTHLTGDIVWLDARGGVGDDFTNGPAQTMSSIDAQ
                     GLGVNGRDTGVGLSGTVIHVGTGLAPFGSDSGMLGELAALSPQRVPLSPSPNASFTGP
                     RLVIGALTLDGDYLFLQSDNMDITQPVSAPTSLLVNLRPSSPRATI"
     gene            complement(2708..5662)
                     /locus_tag="VHE37_16380"
     CDS             complement(2708..5662)
                     /locus_tag="VHE37_16380"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HVT37165.1"
                     /translation="MDIRRGLFAGLFGLIVLAAPLPLLANPGGVGATSGLAAANGITG
                     LGTANVQVNQAATHAVINWNDFSIGAGELVQFNQPSATAAILNRVTGGNLSQIYGQMK
                     GNGRVLLVNPAGIFFAQGSQVDVAGLVASTLDITDANFNADHWQFDGVPDGSVPGRVS
                     SAGTITAAAGGFVALLSQGQQNSSGTINAPQGSILLANGSSIFVDPVGDGLMLYQVSA
                     PTPSNYDANGDGTNDAGNDGIYVAASSLLAADGGRVAVEADVPSAQAANQISAVITQG
                     VIRARAATTGSHGEIILVGRGSDAYNAGLLDASGPGNGTVTLRSDTGVSASPPQNLYP
                     CNQNPNPCTTGDGNNPPLIQARNLVLDGVGSVNLDGVTLAVQAMDVTSGGYASFSGDT
                     FSGLQSVTGHVGELDIFNSDISAPALTFNITGGLFFGGSRLSGNTLNAQASDTLSVGN
                     QPNVPSVVPSSLSFTQQVTLGGVNRINLIGARVDTPALALNTGGIASIDTDQFPQLRM
                     VTGTIGELDFFTSTVTTPDLSFDITGNLFIGGATLSGSTLTARASGNLSIGDEPNVPG
                     PPPSSHLSYGQQIQLAAPGRVNIINATLDTPTLIIATSGYASMQNDQLPNLRMLQAQA
                     LQLQLQDMTLNLPALDIDVAGGLLMDGVALSGGSLTMAADGFELGPSLSAAGAARMLD
                     YTGTVSIDSRGGALDILGASVKAGDLKISGSGYVSIDNSMLQATHGMQLSAGADLQFD
                     VNTSPQSTTQISAGSFAARAGGTINLANAVVDVGTEVAAFGEDDGMIGELRDVAPDRV
                     PLAAGPDASFAAPHVVIGNITLQGDYLYLQTNSLDFTGPLPINAPTDLLVNVRPYGNK
                     DTLGLEQDIAYRRTANFGVNQQLNNAFPGTTYIFGGTDYDGVIFAGEAGPIDFGSHDA
                     GVLFMTNGAVAQEAPIQTSGPQFVLAGLTSAPPEADEDQQHRSATEDDDNGGKDHEVD
                     RVTSPAAAKECR"
     gene            complement(5687..>6078)
                     /locus_tag="VHE37_16385"
     CDS             complement(5687..>6078)
                     /locus_tag="VHE37_16385"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HVT37166.1"
                     /translation="GGAWSPDPLPVSAQYSLGGPFSVRGFPVGDRRGDSGYLGTFGLR
                     RSFGTRLRWALRAFADAGGVSSHQVPPGAKKSESLASAGIGADAGYSSGAMQYALTLD
                     VAQPVGSARPSDGSSGPQVYAQLAVSY"
BASE COUNT          860 a         1950 c         2210 g         1058 t
ORIGIN      
        1 gatcgtcgcg cgcggcgaac tcggccgcag gttgaccagc aggctggtag gcgcgctcac
       61 cggctgcgtg atatccatgt tgtcggactg caggaacagg tagtcaccgt cgagcgtcag
      121 tgcgccgatc accagccgcg ggccggtgaa actcgcattg gggctcggcg acagcggcac
      181 gcgctgcggc gacagcgccg ccagttcgcc gagcatgccg ctgtcgctgc cgaacggcgc
      241 cagcccggtg ccgacgtgga tcacggtgcc ggagagaccc acgccggtgt cgcggccgtt
      301 cacccccagg ccctgcgcgt cgatgctcga catggtctgc gccgggccgt tggtgaaatc
      361 gtcgccgacc ccgccgcgcg cgtcgagcca cacgatgtcg ccggtcaggt gcgtgtcagt
      421 gaggaagatg ccgttgaacg gatcggcggt gccgtaagcc gcccgcagcg tcaggtcccg
      481 cgcgtggatg gtggtggact gcagtgacag attctcgccg gcggtgatgc gcacgggcgc
      541 aatcgcctgc ccgccgatga gcgtgtcctg gatcgcggtg cggccactgc tggccaggtc
      601 cagcgaggta ccgaagtcca gcgtgctctg cgcgttggca ttgctgccgc ggatgtcgat
      661 gccggtggcc tgcacctgca gcgtgctgcc gtcgagcttg ctgccggaga tgaacaggtc
      721 gctgagcccg gttgcgctgc cgccatgggt ggtgaagctg atctgcggcg catgcaccgc
      781 ggtgtcgccg atgacgacga cgccggtgtt gacgatgatc gaatcggtga aggtggcgct
      841 cgcggcattt ccagtggtgg cgctgggcgt gaggtcgaac tcgctgggcg tgccgctgcc
      901 gttcacggcc agcgtggtgc cgctgagcgt ggtgccgcgc acctgcaggt ccggcgagtc
      961 gaactgcatg ccggccgtgg tgcgaacgtc gtcgtcctca aggaaggcgc cgccgctgat
     1021 gtgcagcgaa ccggcgtgca gcggcacgcc gcgcgcgtcg accccggcgt ggatatccat
     1081 cagcccggcg gtggtcagcg cgctggcgcc atcgacgttc acgccatcgg cgttgagttg
     1141 catggtcgtc gcggtcagcg tgctgttgtg cacgttgaag ccgtcgtcga actgcacgtg
     1201 atcggtgccg acctgcgtgg tcagcgtggt ggcgttcagc gtgctgttgt cgacgatcgc
     1261 ttcgcgcggc gtggtgaagc tgaccgcgcc tggcgtgctg gacccgatgg ttgcgccttc
     1321 cagcgtgatg cgcgtgctgc cggtgagatc gatcgacgtc gcgtagtcca tggtgctgcg
     1381 caggtcgagg ttggcgccct gcaccaccag cgtgctgccg ctgagcgtgc tgccggtgac
     1441 ggtgacgcgg ccggcatcgg gtgcggcatc gctgggcgtg ccgctggcga ccagggtcag
     1501 cgcggggctg ctcagcgtga cgccgctcag ctccagctgg ttgccctgca ggcgcaccga
     1561 ggcgggattg gcgccgccct ggcccttgac ggtcacaccg ccgaagttga agtcgccgct
     1621 ggaacgcagg tcgatgacgt cagcggcgac agtgacgcgc tgcgggctga agtccgccac
     1681 gtcgatggag aagctgtgcg cgatcacgct cggcggattg ttgccatcgc cggtgtagcc
     1741 gtaatcctgc ggcggtcgca gcgccgcggc ctggtcggtg cggatgctca cgctgccgtt
     1801 gcccggcccg gatgcatcga gctcgccggc ctgcagcacg ccgctgccgc gcgccaccag
     1861 gatgacctgg ccctgctcgt tggtgctcgc ggtttgcgcg cggatcagcc cgccggtgtt
     1921 gcccagcccg gcgtaggtga cgaagtcgtt gtaggtctcc agcgcgatgc ggccgccgtc
     1981 ggcggtgagg gtgccgtggt tgagcacgcc atcattgccg gcgtcgttct ggttcggatc
     2041 ggcgtcggtg ctgtagtgat agtggtcgag tgtgggcgca gtgacctggt acagcatcag
     2101 cccgtcgccc accggatcga cgaagatgct cgagcctgct gccagcagga tgctgccgtg
     2161 gtccgcactg atcgcggtgt cgttctgcac gccgccctgg ccgagcagcg cgacgaagcc
     2221 gccgcgcgct gcgttgatgg tggcgtcgtt gaccacgcgc cccggcacat tgccgtcggc
     2281 cacgccgtcg aattgccagt ggtcggtgtt gaagttggca tcggtgatgt tgagcgtcga
     2341 ggcaaccagg ccgcccacgt cgacctgccc gcctgagcca aacacgatgc cgttcgggtt
     2401 caccagcagc acgcggccgt tggccttcat ctgtccataa atctgcgaca ggttgccgcc
     2461 ggtgacgcgg ttgaggatgg ccgcggtcgc agacggctgg ttgaactgca ccagctcgcc
     2521 gctatcgatg gaaaaatcat tccagttaat caccgcgtgc gtcgcggcct ggttgacctg
     2581 cacgttggcc gtgcccaggc cggtgatgcc gttggtggcc gcgagtcccg aggtggcgcc
     2641 cacgccgctg ggattggccg ccgcagacag cgggaccaac aatacggttg cgaggaaaac
     2701 gcgcatgtca gcggcactcc ttggccgccg cgggcgaggt gacgcggtcg acttcatgat
     2761 ccttgccgcc gttgtcgtca tcctccgtgg ccgaccgatg ctgctggtcc tcgtcggctt
     2821 ccggcggcgc gctggtcagg ccggccagca cgaactgcgg gccgctggtc tggatcggcg
     2881 cttcctgcgc caccgcgccg ttggtcatga acagcacgcc ggcgtcgtgc gagccgaagt
     2941 cgatcgggcc ggcctcgccg gcgaagatca cgccgtcgta gtcggtgccg ccgaatatat
     3001 aggtggtgcc ggggaaggcg ttgttgagct gctggttcac gccgaagttg gcagtgcggc
     3061 ggtaggcgat gtcctgctcc aggcccagcg tgtccttgtt gccgtaaggc cgcacattca
     3121 ccaacaggtc ggtcggcgca ttgatcggca gcggaccggt gaagtccagg ctgttggtct
     3181 gcaggtagag atagtcgccc tgcaaggtga tgttgccgat gaccacgtgc ggcgcggcaa
     3241 agctggcgtc cggtccggcc gccagcggca cgcggtcggg tgccacgtcg cgcagttctc
     3301 cgatcatgcc gtcgtcctcg ccgaaagcgg cgacctcggt gccgacatcg accaccgcgt
     3361 tggccaggtt gatggtgccg ccggcgcgcg cggcgaacga gccggcgctg atctgcgtgg
     3421 tgctctgcgg gctggtgttg acgtcgaact gcaggtcggc gccggccgac agctgcatgc
     3481 cgtgcgtagc ctgcagcatg ctgttgtcga tgctcacgta gccgctgccg ctgatcttga
     3541 gatcgcccgc cttgacgctg gcgcccagga tgtcgagcgc gccgccgcgc gagtcgatcg
     3601 acaccgtgcc ggtgtagtcg agcatgcgtg cggccccggc cgcgctaagg ctggggccga
     3661 gctcgaatcc gtccgcggcc atggtcagcg agccgccgga caaggccacg ccgtccatca
     3721 gcagtccgcc ggcgacatcg atgtccagcg ccggcaggtt cagcgtcatg tcctgcagct
     3781 gcagctgcag cgcctgcgcc tgcagcatgc gcagattcgg cagctggtcg ttttgcatcg
     3841 acgcgtagcc ggaagtggcg atgatgagcg tgggcgtatc gagcgtggcg ttgatgatgt
     3901 tgacgcggcc cggcgccgcc agctggatct gctggccgta gctcaggtgg ctcgacggcg
     3961 gcggaccggg cacattgggc tcgtcgccga tggacaggtt gccgctggcg cgcgcggtca
     4021 gcgtgctgcc cgacagcgtg gcgccgccga tgaacaggtt gccggtgatg tcgaaggaaa
     4081 gatccggcgt ggtcacggtg gaggtgaaga agtcgagttc gccgatggtg ccggtcacca
     4141 tgcgcagctg cgggaactgg tcggtgtcga tcgaggcgat gccgccggtg ttcagcgcca
     4201 gcgccggcgt gtcgaccctg gccccaatca ggttgatgcg gttcacgccg ccaagcgtga
     4261 cctgctgggt gaagctgagc gaactcggaa ccacgctcgg aacgttcggc tggttgccga
     4321 cgctcagcgt gtcgctggcc tgcgcattga gcgtgttgcc cgacaggcgc gagccgccga
     4381 agaacagtcc gccggtgatg ttgaaggtga gcgctggtgc gctgatgtcg gagttgaaga
     4441 tgtcgagctc gccgacgtgg ccggtcactg actgcaggcc gctgaaagta tcgccgctga
     4501 aggaggcata gccaccgctg gtcacgtcca tggcctgcac ggcgagtgtg acgccatcga
     4561 gattgaccga gccgacgccg tccagcacga ggttgcgtgc ctggatcagc ggcgggttgt
     4621 tgccgtcgcc ggtggtgcag gggttgggat tctggttgca gggatacagg ttttgcggcg
     4681 gcgaggcact gacgccggtg tcgctgcgca gggtcaccgt gccgttgccg ggaccggagg
     4741 cgtcgagcag gccggcgttg tacgcgtcgg aaccgcggcc gaccaggatg atctcgccgt
     4801 gcgagccggt ggtggcggcg cgcgcgcgga tcacgccctg ggtgatcacc gcggagatct
     4861 ggttggctgc ctgcgccgaa ggcacatcgg cctcgacggc gacgcgcccg ccatctgcgg
     4921 cgagcagtga cgaggccgcc acgtagatgc cgtcattgcc cgcatcgttg gtgccgtcac
     4981 cgttggcgtc gtagttcgac ggtgtcggcg cgctgacctg gtacagcatc agcccgtcgc
     5041 ccaccgggtc gacgaagatg ctcgagccgt tggccagcag gatgctgccc tgcggcgcgt
     5101 tgatggtgcc gctgctgttc tgctggccct gcgacagcag cgcgacgaat ccgcccgctg
     5161 cggcggtgat ggtgccggcg ctgctcacgc gcccgggcac gctgccgtcg ggcacgccgt
     5221 cgaactgcca gtggtcggcg ttgaagttgg cgtcggtgat gtcgagcgtg gaagcgacca
     5281 gcccggccac atccacctgg ctgccctgcg cgaagaagat gcccgcggga ttcaccagca
     5341 gcacacggcc gttgcctttc atctgtccat agatctgcga aaggttgccg ccggtgacac
     5401 ggttgaggat cgccgctgtc gcggacggct ggttgaactg caccagctcg ccggcgccga
     5461 tggaaaaatc attccagttg atcaccgcgt gcgtcgcggc ctggttgacc tgcacgttgg
     5521 ccgtgcccag gccggtgatg ccattggccg cggcgagtcc tgaggtggcg cccacgccgc
     5581 cgggattggc cagcagcggc aggggcgcgg cgagcacgat caggccgaac aaaccagcga
     5641 acaatccacg tctgatgtcc atgggtttgt tctccgtctc cgtcactcag taactcaccg
     5701 ccagctgcgc gtagacctgt ggcccgctgc tgccgtcgct cggccgcgcg ctgcccaccg
     5761 gctgcgccac gtcgagcgtg agcgcgtact gcattgcgcc gctgctgtag ccggcatcgg
     5821 cgccgatgcc ggcgctggcc agcgattcgc tcttcttcgc gcccggcggc acctggtggc
     5881 tggacacgcc gccggcatcg gcgaatgcgc gcagcgccca gcgcagccgc gtgccgaaac
     5941 tgcgccgcag gccgaaggtg cccaggtagc cgctgtcgcc gcggcggtcg ccgacgggaa
     6001 atccgcgcac gctgaacggg ccgccgagcg agtactgcgc cgagaccggc aacgggtccg
     6061 gcgaccacgc accgccgg
//
LOCUS       DASZZC010000764         5660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_883933,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000764 DASZZC010000000
VERSION     DASZZC010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883933"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VHE37_16390"
     CDS             complement(<1..348)
                     /locus_tag="VHE37_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVT37167.1"
                     /translation="MSPIRIGISGWRYAPWRGRFYPKGLPQARELAYASRHFSTIELN
                     GSFYSLQRPEHYQRWYDETPPGFVFAIKGSRYITHMLRLKGIEAALPNFLASGIFRLR
                     EKIGPFLWQFPPNL"
     gene            complement(449..907)
                     /locus_tag="VHE37_16395"
     CDS             complement(449..907)
                     /locus_tag="VHE37_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT37168.1"
                     /translation="MLTLQEISDRAELTQLVTDYAYAIDERAWERLDRVFTPDAYIDY
                     RAMGGIDGKYPAVRAWLPDALKHFPGYMHFIGNLSFEISGDRARGKVACFNPMVVPDP
                     NGGSDTMFLGLWYYDEYTRTPQGWRISRREEKKSYDYNMPDWMKKALKLA"
     gene            complement(1054..1431)
                     /locus_tag="VHE37_16400"
     CDS             complement(1054..1431)
                     /locus_tag="VHE37_16400"
                     /EC_number="5.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004769 - steroid delta-isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0008202 - steroid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="steroid Delta-isomerase"
                     /protein_id="HVT37169.1"
                     /translation="MPTEAQMKQAMQAYIDLFNKVDAAGIAELYADDATVEDPVGSPL
                     KKGKAEIRAFYEYAIKTGAKLSLAAPIRGSHGNAAAMAFDVNLKMPTGQAVIRVIDVM
                     TFTESGKFASMKAYWGPGDMTPV"
     gene            1601..2815
                     /locus_tag="VHE37_16405"
     CDS             1601..2815
                     /locus_tag="VHE37_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT37170.1"
                     /translation="MPTAKTPASSTAAARPVAAGLPAVKSAADLQKLDKLIGAIYDGP
                     TETPPWSSALQLMRDALSASHVTLMLRPPSSDSTGVMINTGTVNPQATASYETHFFSL
                     DPFVRLGEGEVVTAEELIGKQWLQSEMYQEFLKPLDIRHLLGADINTKEGIECRLRVT
                     RSHDAQPFSAEDKALVRFLLPHLKRSVLLHARLDLLECERALFAGTVNKLLLGIISFA
                     ANGSIVEMNQEARRILAEKDGIWLSGNNLCVELAAESRELQRIMRAAQTAGAGAPAGE
                     EGPTVVEGMSVSRPSGRAKLGILVRAIPMGEFSESRQRPAYAVFLRDPESNVSQPSTE
                     LLRRLFGLTRMEAQLALLLTEGNTLDEAAEKMDVRRNTARTHLRSIFCKTGVTRQTML
                     VRLLLNSVVTLG"
     gene            complement(2818..3168)
                     /locus_tag="VHE37_16410"
     CDS             complement(2818..3168)
                     /locus_tag="VHE37_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37171.1"
                     /translation="MDAISVVSSAVPNTPAGVGAMPSGLAILIWAPLVLLVAVGIGWL
                     ALSAWLDRRREQRAARAVPRTLDIPLEFLRPDPASATKMVETAPRRLPAAWLDLDLDF
                     GPPPVAGGRRVRPL"
     gene            3400..3912
                     /locus_tag="VHE37_16415"
     CDS             3400..3912
                     /locus_tag="VHE37_16415"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT37172.1"
                     /translation="MKPPILNLADVKLVPLKERMPTQPPGGFGRKFDGRMGEIGGPLG
                     SQKLGYNITAVPPGMRAFPFHNHRVNEEMFFILEGSGQLRVGPDTYPVKQGDFIASLP
                     GGPELAHQLVNTGDGELRYLAVSTRLTPEIVDYPDSGKFGVSMMGADGKPQFRQLGRR
                     EQALDYWDGE"
     gene            3971..4309
                     /locus_tag="VHE37_16420"
     CDS             3971..4309
                     /locus_tag="VHE37_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT37173.1"
                     /translation="MKHQTAVARLAALAQGSRLAVFRLLVKKGHQGLCVGDIQSALKL
                     SPATLSFHLKGLLHAGLLTARQQSRHIYYLPDFDAMNELLGYLTDNCCNGEPCAVNCN
                     PQGRRTRKPS"
     gene            4306..5007
                     /locus_tag="VHE37_16425"
     CDS             4306..5007
                     /locus_tag="VHE37_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HVT37174.1"
                     /translation="MSASRALAAEFVGTALLLAAVVGSGIMAERLAAGNVALALLANS
                     LATGGALIALILCFGPVSGAHFNPAVTLMDATQGGIGAARVSGYIAVQVAGACAGVAA
                     AHLMFGQPVYTFSSHVRAGPSQLFSEFIATFGLLSTIWGCSRSRPQAAPHAVAAYIVA
                     AYWFTASTSFANPAVTLARALTDTFAGIRPVDVMPFIVAQLAGAATATWLFRWLAPLA
                     PARVMVPHEAEDAAP"
     gene            5004..5405
                     /locus_tag="VHE37_16430"
     CDS             5004..5405
                     /locus_tag="VHE37_16430"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HVT37175.1"
                     /translation="MKKIIFACVHNAGRSQMSAAFFNALVDPAHAHAVSAGTQPAARV
                     HPQVLQAMRDAGIDLAGARPQKLTAELAQDAHLLITMGCGDECPYVPGLRREDWPLPD
                     PKGQSADRVLQIRDDIRQRVIELLEREGWSR"
     gene            complement(5412..>5660)
                     /locus_tag="VHE37_16435"
     CDS             complement(5412..>5660)
                     /locus_tag="VHE37_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37176.1"
                     /translation="ACAVALWMQCLRDEIASAQYADAHKDQLMALARSGDAAGFVSFA
                     LALPVEHAAAFSAQVAQQLSALSAGVQAHLRKVAAAVS"
BASE COUNT          976 a         1899 c         1841 g          944 t
ORIGIN      
        1 caggttcggc gggaactgcc acaggaacgg tccgatcttc tcgcgcaggc ggaagatccc
       61 ggaggcgagg aaattcggca gcgccgcctc gatgcccttg agccgcagca tatgcgtgat
      121 gtaacggctg cccttgatgg cgaagacgaa acccggcggc gtttcgtcgt accagcgctg
      181 gtaatgctcg ggccgctgca gcgagtagaa cgaaccgttg agttcaatgg tggaaaaatg
      241 ccgcgaggcg tatgccagct cgcgcgcctg cggcaagcct ttcgggtaga acctgccgcg
      301 ccacggtgcg tagcgccagc cggaaatgcc gatgcggatt gggctcatga acatcagagc
      361 cagcgccagc ctgatttgtt ctgcaggagc gcgccttgag ggtgatcgat gtcgcctatc
      421 gcgcgcaagg cgcggcccca caaccccgtc aggcgagctt cagcgccttc ttcatccagt
      481 ccggcatgtt gtagtcgtag ctcttcttct cttcgcgccg gctgatgcgc cagccctgcg
      541 gcgtgcgcgt gtactcgtcg tagtaccaca ggccaaggaa catggtgtcg ctgccgccgt
      601 tcgggtcggg caccaccatc gggttgaagc acgcgacctt gcccctggcg cggtcgccgg
      661 aaatctcgaa ggagagattg ccgatgaagt gcatgtagcc gggaaaatgc ttgagcgcat
      721 ccggcagcca ggcgcgcact gcggggtact tgccgtcgat gccgcccatg gcacggtagt
      781 cgatgtaggc gtcgggcgtg aacacgcggt ccagccgctc ccacgcgcgc tcgtcgatgg
      841 cgtaggcgta atcggtgacc agctgggtga gttccgcgcg gtccgagatt tcctgcagcg
      901 tcagcatgtg cgcgctcctg cggcgtgttg catgggaaaa aacgaggtca ccgcacccgc
      961 atttctgatg ttattaaata ttgatttaat ttaaatatat acaataatta ataacatcac
     1021 agatgccggt gcggtggagc aaaaaaacgc cgttcagacc ggcgtcatgt cccccgggcc
     1081 ccagtacgcc ttcatcgagg cgaacttgcc ggactcggtg aaggtcatca cgtcgatcac
     1141 gcggatcacc gcctggccgg tgggcatttt caggttcacg tcgaaggcca tcgccgccgc
     1201 gttgccgtgc gagccgcgga tcggcgcggc cagcgacagc ttggcgccgg tcttgatcgc
     1261 gtactcgtag aaggccctga tctcggcctt gcccttcttc agcggcgagc cgaccggatc
     1321 ttccaccgtg gcgtcgtcgg catacaactc agcgatgccg gcggcgtcga ctttgttgaa
     1381 caggtcgata taggcctgca tggcctgctt catctgcgct tcggtcggca cggtgctccc
     1441 ccgcgtgaaa gtggaaaaat gtttggtgcg tcatgttcgt gcgcgaacca cggcggcaaa
     1501 tcgtccggaa agacgagagc gcaccgttac ggcgctatac tcagccgaac cagcaatatc
     1561 gccgaatccg gtgcctccaa tcggagcagc agcagcgccc atgcccactg ccaagacccc
     1621 agcctccagc acggccgcgg cgcggccggt ggctgccgga ctgccggcgg tgaagagtgc
     1681 ggctgatctg cagaagctgg acaagctgat cggcgccatc tacgacggtc ccaccgaaac
     1741 gccgccgtgg agcagcgcgc tgcagctgat gcgcgacgcg ctgtccgcct cgcatgtcac
     1801 gctcatgctg cggccgccgt ccagcgattc caccggcgtg atgatcaaca ccggcacggt
     1861 caatccgcag gccaccgcct cctacgaaac gcatttcttc tcgctcgatc cgttcgtgcg
     1921 cctgggcgaa ggcgaagtgg tcaccgccga ggaactgatc ggcaagcagt ggctgcagag
     1981 cgagatgtac caggaattcc tcaagccgct ggacatccgc catctgctcg gcgccgacat
     2041 caacaccaag gaaggcatcg agtgccgcct gcgcgtgacg cgctcacacg acgcccagcc
     2101 cttcagcgcg gaggacaagg cgctggtgcg cttcctgctg ccgcacctga agcgctcggt
     2161 gctgctgcac gcgcggctgg acctgctcga gtgcgagcgc gcgctgttcg ccggcacggt
     2221 caacaagctg ctgctgggca tcatcagctt cgccgccaac ggcagcatcg tcgagatgaa
     2281 ccaggaagcg cgccgcatcc tggcggagaa ggacggcatc tggctctctg gcaacaatct
     2341 ctgcgtggag ctggccgcgg agtcgcgcga actgcaacgc atcatgcgcg cggcgcagac
     2401 cgccggcgcc ggcgcgccgg ccggcgagga aggccccacc gtggtcgaag gcatgtcggt
     2461 gtcgcgcccg tcgggccgcg ccaagctcgg catcctggtg cgcgccatcc ccatgggcga
     2521 attctccgag agccgccagc gtccggccta cgccgtgttc ctgcgcgacc cggaatccaa
     2581 cgtgtcgcag ccgtccaccg agctgctgcg ccgcctgttc ggcctcaccc gcatggaagc
     2641 gcagctggcg ctgctgctca ccgagggcaa cacgctcgac gaagccgcgg aaaagatgga
     2701 cgtgcgccgc aacaccgcgc gtacccacct gcgctccatc ttctgcaaga ccggcgtcac
     2761 ccggcagacc atgctggtgc ggctgctgct caacagcgtg gtcacgctgg gctgagccta
     2821 gagtggccgc acgcgccggc cacctgcgac cggcggtggt ccaaaatcca ggtccagatc
     2881 gagccaggcc gcaggcaggc ggcgcggcgc ggtttccacc atcttcgtcg cgctcgccgg
     2941 gtccggccga aggaactcca gcgggatgtc gagtgtgcgc ggcaccgccc tcgccgcacg
     3001 ctgctcgcgc cggcggtcga gccaggcgct cagcgccagc cagccgatgc cgacggccac
     3061 cagcagcacc agcggcgccc agatcaggat ggccaatccc gaaggcatcg cgcccacgcc
     3121 tgcgggggta ttgggaacgg cgctggaaac cacgctgatc gcgtccatct gcattgctcc
     3181 ttgtcgcacc tgcttgtgcg gcagctgcag atcaacagag cgcgcggcgc cgggcaatgg
     3241 gcggtgcgtg atgcagcccg caatgccgct gtaggaattt cgcgcgcaaa cagggcgcag
     3301 tgtgagcgcg cccgcagatg tcgtggaaaa tgcgcggcgg ctcacacttg cgcgaagtgc
     3361 gggacaatcc cacatctgga taggtccaag gaggaaccca tgaaaccgcc catcctcaac
     3421 cttgccgacg tcaagctggt gccgctgaag gagcgcatgc cgacccagcc gccgggcggc
     3481 ttcggccgga agttcgacgg ccgcatgggc gagatcggcg gcccgctggg ttcgcagaag
     3541 ctcggctaca acatcaccgc ggtgccgccc ggcatgcgcg cgtttccgtt ccacaaccac
     3601 cgcgtcaacg aggagatgtt cttcatcctc gaaggcagcg gacagctgcg cgtggggccg
     3661 gacacctacc cggtgaaaca gggcgacttc atcgcctcgc tgcccggcgg gccggaactg
     3721 gcgcaccagc tggtcaacac cggcgacggc gagctgcgct acctggcggt gagcacgcgg
     3781 ctgacgccgg agatcgtcga ttacccggat tcgggcaagt tcggcgtgtc gatgatgggt
     3841 gccgacggca agccgcagtt ccgccagctg gggcgcaggg aacaggcgct ggattactgg
     3901 gacggcgagt aggcgcgatc gtcgtctttc gcgattgacc ttgccaattt atttccatta
     3961 ttctcgaaac atgaaacatc agacggcggt ggcccggctc gcggcactgg cgcaggggtc
     4021 gcgcctggcg gtgttccggc tgctggtgaa aaaggggcat cagggcctgt gcgtcggtga
     4081 catccagtcc gcgctcaagc tgtccccggc caccctgtcg tttcacctga agggcttgct
     4141 gcatgccggc ctgctgaccg cccggcagca aagccgccac atctattacc tgccggattt
     4201 cgacgcgatg aatgaactgc tcggctacct gacggacaac tgctgcaacg gcgagccctg
     4261 cgccgtgaac tgcaatcccc agggcaggcg cacccggaag ccgtcatgag cgcatcgcgc
     4321 gcccttgccg ccgaatttgt cggaaccgcc ctgctgctcg ccgccgtggt gggctccggc
     4381 atcatggccg agcggctggc agccgggaac gtcgcgctcg cattgctggc caattcgctg
     4441 gccacgggtg gcgcgctgat cgcgctgatc ctgtgtttcg gcccggtctc cggtgcgcac
     4501 ttcaacccgg cggtgacgct gatggatgca acgcagggcg gcattggcgc ggcgcgtgta
     4561 tcgggctaca tcgcggtgca ggttgccggc gcctgtgcgg gcgtggcggc tgcgcacctg
     4621 atgttcggcc agccggtcta caccttttcc agccatgtcc gcgctggccc gtcgcagctt
     4681 ttctctgaat tcatcgcaac cttcggcctg ctgtcgacca tctggggctg ctcgcgctcg
     4741 cggccccagg cggctcccca tgcagtggcg gcgtacatcg tggcggcgta ctggttcacc
     4801 gcctcgacct cgttcgccaa tccggcggtg acgctggcac gggctctgac cgacaccttc
     4861 gccggcatcc ggccggtcga cgtcatgcca ttcatcgtcg cgcagctcgc aggcgctgca
     4921 accgccacct ggctgttccg ctggctcgcg ccgctcgcgc cagcgcgggt gatggtgccg
     4981 cacgaagcgg aggatgcggc gccatgaaga agatcatctt cgcctgcgtg cacaacgccg
     5041 gccgctcgca gatgtcggcg gcattcttca atgcgctggt cgatcccgcc catgcgcatg
     5101 cggtctctgc cggcacgcag ccggccgcac gcgtgcatcc gcaagtgctg caggccatgc
     5161 gcgacgcagg catcgacctg gccggcgcac gcccgcagaa actaaccgcc gaactcgcgc
     5221 aggacgcaca cctgctgatc accatgggct gcggcgatga gtgcccgtac gtgcccggcc
     5281 tgcggcgcga agactggccg ctgcccgatc ccaagggaca atccgccgac cgcgtgctgc
     5341 agatccgcga cgacatccgc cagcgcgtga tcgaactgct ggagcgcgaa ggctggagcc
     5401 gttgaaccgc ttcagctgac cgccgcggcc accttgcgca ggtgagcctg cacaccggcg
     5461 gatagagcag agagctgctg cgccacctgc gcggaaaacg ccgcggcatg ttcgacgggc
     5521 agtgcgagcg cgaatgagac gaagcccgcc gcatcgccgc tgcgcgccag cgccatcaac
     5581 tggtccttgt gcgcatcggc atattgcgcg ctggcgattt cgtcgcgcag gcattgcatc
     5641 cacagcgcga cggcacaggc
//
LOCUS       DASZZC010000765         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_575743,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000765 DASZZC010000000
VERSION     DASZZC010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575743"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VHE37_16440"
     CDS             complement(<1..389)
                     /locus_tag="VHE37_16440"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HVT37177.1"
                     /translation="MSAIILIDIGNSRLKWARAQGTRLARGGVVEHAGDPAGALARIE
                     LPRADAVWIAHVTGAQHEAGLTRAAQSACGLAPRYARSEKKRLGLTNAYREPERLGVD
                     RWLALLAAWSLHRGALCVADAGTALTV"
     gene            complement(386..1351)
                     /locus_tag="VHE37_16445"
     CDS             complement(386..1351)
                     /locus_tag="VHE37_16445"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HVT37178.1"
                     /translation="MAVALSEAELLALADALADGAWHSGEDLAAQAGITRAALAKRID
                     RLRDWQLDIETRHGLGYRLAAPLERLDEKKLRAALPRELGVRVLALTDSTNTQLLGAD
                     AAHDPQALLAEFQTGGRGRRGRAWVSPFGANLYFSLAVSFSAWPRDLPALSLAVGVAS
                     ARVLRAHGAEVGLKWPNDLWIGGRKLGGILIEHRGEAGGACRAVIGVGLNLAMSRAQA
                     GAIDQPWIALEEALNRRVPRNELAAALLAALLETVTDFEREGFAPFVADWNRFDLTAG
                     RSVRVEREPPLSGIARGIDASGALIVEAGGQRHAIVSGEVSLRLA"
     gene            complement(1336..2001)
                     /gene="plsY"
                     /locus_tag="VHE37_16450"
     CDS             complement(1336..2001)
                     /gene="plsY"
                     /locus_tag="VHE37_16450"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HVT37179.1"
                     /translation="MLELIIKFLGAYLLGNLLGGEVVGALSGGVNLRSVGSGNIGATN
                     ALRTQGKGFALAVLAIDIFKGVAAVTFIPAIDWHWVARLYWNPVWQEYLCGVAVTLGH
                     CYPLRHRFAGGKGVATLAGVYGALLPWSLPWLLGSFVLVILLTGYASLATLTASVMAM
                     FFVACISHDGMFSPAGTFTIVMALLVAWKHRDNIRRLLRGEESRFERARVLGRRIEQW
                     LSR"
     gene            complement(2006..>2301)
                     /locus_tag="VHE37_16455"
     CDS             complement(2006..>2301)
                     /locus_tag="VHE37_16455"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVT37180.1"
                     /translation="EARIAHVLAGSPAARAGLAANDLLLALDGLRLSAGTLGPRLAAM
                     TPGRDIALHYFRGDELCNVKLALAAPPKDTWALTLAASPNTAVLARRKAWLGA"
BASE COUNT          349 a          841 c          812 g          299 t
ORIGIN      
        1 tcgacggtca gcgcggtgcc cgcatcggcc acgcacagcg cgccgcggtg caaactccat
       61 gcggcgagca gggccagcca gcggtcgacg cccagccgtt ccggctcgcg gtaggcattg
      121 gtcaggccca ggcgcttttt ctcgctgcgc gcataacgcg gcgcaagtcc gcaggcggat
      181 tgcgccgcgc gcgtgagccc ggcctcatgc tgcgcgccgg tcacgtgcgc gatccacacg
      241 gcgtccgcac gcggaagctc gatgcgcgcc agcgcacccg ccggatcgcc ggcgtgttct
      301 accaccccac cgcgcgcgag gcgcgtgccc tgcgcgcgcg cccacttgag ccgcgagttg
      361 ccgatgtcga tgaggatgat cgcgctcatg ccagccgcag gctgacttcg cccgagacga
      421 tggcgtggcg ctgcccgccc gcctcgacga tcagcgcgcc gctggcgtcg attccgcgtg
      481 caatgccgct gagcggcggc tcgcgctcca cgcgcacgct gcgtccggcg gtgaggtcga
      541 agcggttcca gtcggcgaca aacggagcga agccttcacg ctcgaaatcg gtgacggttt
      601 cgagcaaggc cgccagcagc gcggcggcca gctcattgcg cggcacgcgg cggttcagcg
      661 cttcctccag cgcaatccac ggctggtcga tggcgccggc ctgcgcacgc gacatggcca
      721 ggttcaagcc cacgccgatc accgcgcggc aggcgccgcc ggcttcgccg cgatgttcga
      781 tgaggatgcc gccgagcttg cgtccgccga tccacaggtc gttgggccac ttgaggccga
      841 cttcggcgcc gtgcgcgcgc agcacgcgcg cgctggcaac gcccaccgcc agcgacagcg
      901 ccggcaggtc gcgcggccac gcggagaagc tgaccgcgag cgagaaatag agattggcac
      961 cgaacggcga gacccaggcg cgcccgcgcc ggccgcggcc gccggtctgg aattcggcca
     1021 gcagcgcctg cggatcgtgc gccgcatcgg cgccgagcag ctgcgtgttg gtggaatcgg
     1081 tcagcgccag cacgcgcacg cccagttcgc gcggcaacgc ggcgcgcagc tttttttcgt
     1141 cgagccgctc cagcggcgcg gccagtcgat aacccaggcc atggcgcgtc tcgatgtcca
     1201 gctgccagtc gcgcaggcga tcgatgcgct tggccagcgc agcgcgggtg atgccggctt
     1261 gcgcggccag gtcttcgccc gaatgccagg cgccgtcggc cagcgcgtcg gccagcgcca
     1321 gcagctcggc ctcgctcagc gcgacagcca ttgctcgatc cgccggccga gcacgcgcgc
     1381 gcgctcgaag cggctctctt cgccgcgcag caggcggcgg atgttgtcgc ggtgcttcca
     1441 cgccaccagc agcgccatga cgatggtgaa ggtgccggcc ggcgagaaca tgccatcgtg
     1501 cgaaatgcag gcgacgaaga acatcgccat caccgacgcg gtgagcgtgg ccaggctggc
     1561 gtaaccggtg agcaggatga ccagcacgaa gctgccgagc agccacggca gcgaccacgg
     1621 cagcagcgcg ccgtaaacgc cggccagcgt ggccacgccc ttgccgccgg cgaagcggtg
     1681 gcgcagcggg tagcaatggc ccagcgtcac cgccacgccg cacaggtatt cctgccagac
     1741 cgggttccag tacaggcgcg ccacccagtg ccagtcgatc gccgggatga aggtgaccgc
     1801 ggccacgccc ttgaagatgt cgatggccag caccgccagc gcgaagccct tgccctgcgt
     1861 gcgcagcgcg ttggtggcgc cgatgttgcc gctgcccacg ctgcgcaggt tgacgccgcc
     1921 ggacagcgcg ccgaccacct cgccgccgag caggttgccc agcaggtagg cgccgaggaa
     1981 cttgatgatc agttcgagca tgcgctcacg cgccgagcca ggccttgcgc cgcgccagca
     2041 ccgccgtgtt gggcgatgcg gcgagcgtca gcgcccaggt gtccttgggc ggagcggcca
     2101 gcgcgagttt cacgttgcac agttcatcgc cgcggaagta atgcagcgcg atgtcgcgcc
     2161 ccggtgtcat cgccgccagc cgcggaccca gcgtgccggc cgacaggcgc aggccgtcga
     2221 gcgccagcag caggtcgttg gccgccaggc ccgcgcgcgc ggccgggctg ccggcgagca
     2281 cgtgcgcaat gcgcgcctcg c
//
LOCUS       DASZZC010000766         8314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_440218,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000766 DASZZC010000000
VERSION     DASZZC010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440218"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VHE37_16460"
     CDS             complement(<1..458)
                     /locus_tag="VHE37_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVT37181.1"
                     /translation="MFRTTDVSALDKPALYDELALQARALFEGERDRIANAANLSALI
                     WHALPQLNWAGCYFFDGRELVLGPFQGKPACVRIAIGKGVCGTAAAQRATQIVADVHA
                     FPGHIACDAASRSEIVVPLLRSHELLGVLDLDSPVLGRFDDADARGLERLA"
     gene            687..2741
                     /locus_tag="VHE37_16465"
     CDS             687..2741
                     /locus_tag="VHE37_16465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HVT37182.1"
                     /translation="MKKIVSGAACAVLLAACSKPAPAPEAAPPPPAPKLVSGIEPGNN
                     DPAVRAQDDLYRAVDGQWLAKTEIPADKSNYGAFTKLADDAEAQLRAIIEAAAKNPNK
                     KAGSEEQKIGDLYTSFMDEAKVEEAGVKPLEPELALIDGLKSKKDIAQLLGHDLLLQV
                     PAPVAAEVHQDAKDPSKYIIDFKQGGLGLPDRDYYLSGDAKFKEIRTQYLAYIEKMYG
                     LAGLKDGARAAKAVLELETKLAQIQWPRVETRDAVKTYNPQDAAKLAALTPALDWKAL
                     LKEGQIPEQPFYVVSEPNYLSGLSKLISSVPLADWQSYFRFKLINDRAPLLSKALVDE
                     NFHFNGTVLNGIAENRPRWKRGVQMIEVALGEAVGHIYVDQHFPPEAKARMDQLVGNL
                     LAAYKQGIDGLEWMGADTKKAAQEKLAKFMPKIGYPKKWKDYSALTVAPDDLYGNMTR
                     SAQVEALRQINKLGKPVDRDEWGMTPQTVNAYYNPELNEIVFPAAILQPPFFNMKADD
                     AVNYGGIGAVIGHEISHGFDDQGARYDGDGVLRDWWTADDLKNFKAKTEALIAQYSSY
                     EPLPGVKLNGAFTIGENIADLSGMTIAYKAYKLSLNGKPAPVIDGLTGEQRFFMGWAQ
                     VWRRKYKDENLLARIKTDPHSPSEFRCNGVVINMPEFDAAFGVKEGDKLYKPAAEQIK
                     IW"
     gene            2800..4860
                     /locus_tag="VHE37_16470"
     CDS             2800..4860
                     /locus_tag="VHE37_16470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVT37183.1"
                     /translation="MTQLRILATACVLLAACSQPQPPAAAPSPVAAPPAAPAPVAGVS
                     RSDGDPAVRAQDDFYRAVNGGWYDRTEIPPEKAEYGSFDIVFDDTERQLRELVETAAS
                     AHGAAGAEAQKVGDLYASYMDQARVEELGLKPVQPELERIDAIKDKAELPALMAHLQR
                     IGVALPFNTVVHQDNKDATRYIVDFQQSGLGMPDRDYFLDQNPKFAQLRKDYLAHIEK
                     MLALSGAKDIEAGARGVLAFETALAGKQWDNVSTRDPLKVYNLLDAAKFKALMPGYGW
                     DAYLKDAGIAVDSMVVSEPSYLAALDRILRDTPLPVLKNYFKLRLLDGSALVLNQQVY
                     DEAFDFNRAKLYGVKAQKPRWKRGIKLVDASIGEALGKIYVEKYFPPANKARMEQLVA
                     NLLKAYDQEFDTLAWMSDDTRRRAKAKLAKFMPKIGYPKKFRDYSALVIARGDLYGNF
                     VRANEFEYQREIGKLGRPVDRDEWGMTPQTINAYYNPELNEIVFPAAILKPPFFDMAA
                     DDAVNYGGIGAVIGHEISHGFDDQGSLYDGDGNLQDWWTPEDRRKFQEQTRALIAQYD
                     QYEVVKGFHVKGALTIGENIADLGGITIAYKAYQISLGGKPAPVIDGLSGDQRFFMGY
                     AQIWREKLRDEAAINLVKSDPHSPGRFRCNGVVINVPEFYSTFDVKPGDKMYSPPEKR
                     VKIW"
     gene            4971..5411
                     /locus_tag="VHE37_16475"
     CDS             4971..5411
                     /locus_tag="VHE37_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HVT37184.1"
                     /translation="MYKHSLMSISAVLALALMTGCASVPMATPEADAQAKTFKAPADK
                     ANLYIYRNESFGSAVKMTVLLDNQLAGDTGPHTYILKSVAPGSHTLTSKTENDATLTI
                     DAAAGKNYYVWQEVKMGALSAGSQLHLVDDTAGQAGVQDCKLVQ"
     gene            complement(5446..5781)
                     /locus_tag="VHE37_16480"
     CDS             complement(5446..5781)
                     /locus_tag="VHE37_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HVT37185.1"
                     /translation="MADRESYQRIWDVVRKIPRGRVATYGQVAAEAGYPKQPRLAGYA
                     MFNIPPRLKLPWQRVINAQGRISFPPRSERWRAQKKLLEAEGVVFLRGRVNLEKFLWR
                     PRSVAPVLD"
     gene            complement(5807..6067)
                     /locus_tag="VHE37_16485"
     CDS             complement(5807..6067)
                     /locus_tag="VHE37_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37186.1"
                     /translation="MLATSGRLEAALMDVVQRAGMGSGLPIRFALIAQEWNRIGLRAS
                     ELRDAVRELVERRYATCTQQDGQLAIAPTPSGLRRLAESEDE"
     gene            complement(6190..6537)
                     /locus_tag="VHE37_16490"
     CDS             complement(6190..6537)
                     /locus_tag="VHE37_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37187.1"
                     /translation="MGSLAALSEWIEQHEALLASHLLINPADASGHRGLCAAFLSRAT
                     DGAFHLRLCEGANDEFLTWRHQRKVQPMFGRSYAEATLGTWLSEREREGYRATWSATL
                     HDTPASMGMAVPA"
     gene            complement(6765..6992)
                     /locus_tag="VHE37_16495"
     CDS             complement(6765..6992)
                     /locus_tag="VHE37_16495"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HVT37188.1"
                     /translation="MKRITMGLGLAILAATGICLAGTPCDQVKAKVEDKIKSHGVKAY
                     TLDIVPAGENKDGKVVGTCEGGKKQLVYKRG"
     gene            7182..7565
                     /locus_tag="VHE37_16500"
     CDS             7182..7565
                     /locus_tag="VHE37_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37189.1"
                     /translation="MYISPNDVEAALLRIYAELQIPPGAALSHSYLLKRWRENRLRHS
                     DLDATLNRLIRSRQMDARDSFEGRAFALTPSGQRRARTLRTGPGGRLLRRLRLAWAQV
                     MRRRGSRDGDAAQHRRLIDRAATRI"
     gene            complement(7572..>8314)
                     /locus_tag="VHE37_16505"
     CDS             complement(7572..>8314)
                     /locus_tag="VHE37_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HVT37190.1"
                     /translation="ASGGSGIPPIPAVDFSQFGPVEKKPLARIRKLSAAHLSRAWLNL
                     PHVTQFDEADITEVEAFRKSSAEETGVKLTLLPFIMKAVAKAMQTYPEFNSSLAPGGE
                     ELVLKKYCHIGFAADTENGLVVPVVREVDKKGVVQLAEECGALAKKARDGKLKPDEMK
                     GGCFSISSLGGIGGSHFTPIINAPEVAILGASRAQMKPVWDGKQFQPRLMLPLSLSYD
                     HRVIDGAYAARFIVHLVKLLSDVRRLAL"
BASE COUNT         1630 a         2690 c         2694 g         1300 t
ORIGIN      
        1 gccagccgct ccaggccgcg cgcatcggca tcgtcaaagc ggccgagcac cgggctgtcg
       61 agatcgagca cgccgagcag ttcatggctg cgcagcagcg gcaccacgat ctccgagcgg
      121 gatgccgcat cgcaggcgat atgcccgggg aatgcgtgca cgtccgccac gatttgcgta
      181 gcacgctggg cagcggcggt gccgcacacg cccttgccga tggcgatgcg cacgcaggcc
      241 ggcttgccct ggaacgggcc gagcaccagc tcgcgcccgt cgaagaaata gcaaccagcc
      301 cagttgagct gcggcagcgc gtgccagatc agcgccgaca ggttggccgc gttggcgatg
      361 cggtcgcgct cgccttcgaa cagcgcgcgc gcctgcagcg caagctcgtc gtagagcgcg
      421 ggcttgtcga gcgcggagac gtcggtagtg cggaacatgg acgcgcacag taacaaaatc
      481 cgtccaccgc acccgtgttt tcgatgttag taaatatgta acaaacttat aataagacaa
      541 taattaataa catcgaaaac ggccgtgcgg tgtgaccttt tgcgctgcac ggactgaggg
      601 aatttcccgc agcggacgtt gtcagagcgc tgttaagctg cgccctcttt ttggcgtggc
      661 gctgaacact cagggagaaa tcacagatga agaagatcgt cagcggcgcg gcctgcgccg
      721 tcctgctcgc ggcgtgcagc aagccggcgc cggcaccgga agcagcgccg ccgccgcccg
      781 cacccaagct ggtatcgggt atcgagccgg gcaacaacga tccggcggtg cgcgcgcagg
      841 atgacctgta ccgcgcggtc gacggccagt ggctggccaa gacggaaatc ccggcggaca
      901 agtccaacta cggcgccttc accaagctgg ccgacgatgc cgaggcgcag ctgcgcgcca
      961 tcatcgaggc cgcggccaag aacccgaaca agaaggccgg cagcgaggaa cagaagatcg
     1021 gcgacctgta caccagcttc atggacgagg ccaaggtcga agaagcaggc gtgaaaccgc
     1081 tggagccgga gctggcgctg atcgacggcc tgaagagcaa gaaggacatc gcgcaactac
     1141 tcggccacga cctgctgctg caggttcccg ctccggtggc cgccgaagtg caccaggatg
     1201 ccaaggatcc gagcaagtac atcatcgact tcaagcaggg cggcctgggc ctgccggacc
     1261 gcgactacta cctgtcgggc gatgccaagt tcaaggagat ccgcacgcaa tacctcgcgt
     1321 acatcgaaaa gatgtatggc ctggccggcc tcaaggacgg cgccagggcg gccaaggcgg
     1381 tactcgaact cgaaacaaaa ctggcccaga tccagtggcc gcgcgtggaa acgcgcgacg
     1441 cggtgaagac ctacaacccg caggacgccg ccaagctggc cgcgctgacg ccggcgctgg
     1501 actggaaagc gctgctcaag gaaggccaga ttcccgagca gccgttctac gtggtgagcg
     1561 agccgaatta cctgtcgggc ctgtccaagc tgatcagcag cgtgccgctg gccgactggc
     1621 agagctactt ccgcttcaag ctgatcaacg accgcgcgcc gctgctgtcg aaggcgctgg
     1681 tggatgaaaa cttccatttc aacggcaccg tgctcaacgg catcgccgaa aaccgcccgc
     1741 gctggaagcg cggcgtgcag atgatcgagg tggcgctggg cgaggcggtc ggccacatct
     1801 atgtcgatca gcatttcccg cctgaagcca aggcgcgcat ggaccagctg gtcggcaacc
     1861 ttctggccgc ctacaagcag ggcatcgacg gcctggagtg gatgggcgcc gacaccaaga
     1921 aggcggcgca ggagaagctg gccaagttca tgcccaagat cggctatccg aagaagtgga
     1981 aggattactc cgcgctgacc gtggcgccgg atgacctcta cggcaacatg acgcgctcgg
     2041 cacaggttga ggcgctgcgc cagatcaaca agctgggcaa gccggtcgat cgcgacgagt
     2101 ggggcatgac gccgcagacg gtcaacgcct actacaaccc ggagctcaac gaaattgtat
     2161 ttcccgccgc catcctgcag ccgccgttct tcaacatgaa ggccgacgac gcggtgaact
     2221 acggcggcat cggcgcggtg atcggccacg agatcagcca cggcttcgac gaccagggcg
     2281 cgcgctacga cggcgacggc gtgctgcgcg actggtggac tgccgacgac ttgaagaact
     2341 tcaaggccaa gaccgaggcg ctgatcgcgc agtacagcag ctacgagccg ctgccgggcg
     2401 tgaagctcaa cggcgcattc accattggcg agaacatcgc cgacctgtcg ggcatgacca
     2461 tcgcctacaa ggcctacaag ctgtcgttga acggcaagcc ggcgccggtg atcgacggcc
     2521 tgaccggcga acagcgcttc ttcatgggct gggcccaggt ctggcgccgc aagtacaagg
     2581 acgagaacct gctggcgcgc atcaagaccg atccgcattc gcccagcgag ttccgctgca
     2641 acggcgtggt gatcaacatg ccggagttcg atgctgcgtt cggcgtcaag gaaggcgaca
     2701 agctgtacaa gcccgccgcc gagcagatca agatctggta acgcagtaac gcaccgggcc
     2761 ggcagcagcc ggcccggttc tttttggagg caaaaaacca tgacccagct gcgcatcctc
     2821 gcaacagcct gcgtgctgct tgccgcctgc agccagccgc aaccacccgc ggctgcgccg
     2881 tccccggtgg cggcgccgcc tgcggctccc gccccggtgg ccggcgtcag caggagcgac
     2941 ggcgatccgg cggtgcgcgc gcaggatgac ttctaccgcg cggtcaacgg cggctggtac
     3001 gaccgcaccg agatcccgcc tgagaaggcc gagtacggtt cgttcgacat cgtgttcgac
     3061 gacaccgagc gccagctgcg agagctggtg gaaacagccg ccagcgcgca cggcgcggcc
     3121 ggcgccgagg cacagaaggt cggcgacctg tatgcgagct acatggacca ggcgcgcgtg
     3181 gaggaactcg gcctgaagcc ggtgcagccc gagctggaac gcatcgacgc catcaaggac
     3241 aaggccgaac tgccggcgct gatggcccat ctccagcgca tcggcgtcgc gctgccgttc
     3301 aacacggtgg tgcaccagga caacaaggat gcgacacgct acatcgtcga cttccagcag
     3361 agcggcctgg gcatgcccga ccgcgactat tttctcgacc agaaccccaa gttcgcgcag
     3421 ctgcgcaagg actacctggc gcatatcgag aagatgctgg cgctgtccgg ggcgaaggat
     3481 atcgaagccg gcgcgcgcgg cgtgctggcc ttcgagaccg cgctggcggg caagcaatgg
     3541 gacaacgtca gcacgcgcga tccgctcaag gtgtacaacc tgctcgacgc ggcaaagttc
     3601 aaggcgctga tgccgggcta tggctgggat gcgtacctga aggatgccgg catcgcagtc
     3661 gactcgatgg tggtgagcga gccgagctac ctggcggcgc tggacaggat cctcagggac
     3721 acgccgctgc cggtattgaa aaactacttc aagctgcgcc tgctcgatgg cagcgcgctg
     3781 gtactgaacc agcaggtgta cgacgaggcc ttcgatttca accgcgccaa gctctatggc
     3841 gtgaaggcgc agaagccgcg ctggaagcgc ggcatcaagc tggtcgatgc gtcgatcggc
     3901 gaagcgctgg gcaagatcta cgtggagaaa tacttcccgc cggccaacaa ggcgcgcatg
     3961 gaacagctgg tggccaacct gctcaaggca tacgaccagg aattcgacac gctggcgtgg
     4021 atgagcgacg acacgcggcg cagggccaag gccaagctcg ccaagttcat gcccaagatc
     4081 ggctacccga agaaattccg cgactactcg gcgctggtca tcgcgcgtgg cgacctctat
     4141 ggcaattttg tgcgcgccaa cgaattcgag taccagcggg agatcggcaa gctgggcagg
     4201 cccgtggacc gcgacgagtg gggcatgacg ccgcagacca tcaacgccta ttacaacccg
     4261 gagctcaacg agattgtgtt ccccgcggcc atcctcaagc cgccgttctt cgacatggcc
     4321 gcggacgacg cggtgaacta cggcggcatc ggcgcggtga tcggccacga gatctcgcat
     4381 ggcttcgacg accagggcag cctgtacgac ggcgacggca atctccagga ctggtggacg
     4441 ccggaagacc gcaggaaatt ccaggagcag acgcgcgcgc tgatcgcgca gtacgaccag
     4501 tacgaagtgg tcaagggctt ccacgtgaag ggcgcgctga ccatcggcga gaacatcgcc
     4561 gacctgggcg gcatcaccat tgcctacaag gcgtaccaga tttcactcgg cggcaagccg
     4621 gcaccggtga tcgacggact gtccggcgac cagcgcttct tcatgggtta cgcgcagatc
     4681 tggcgcgaga agctgcgcga cgaggcggcg atcaacctgg tgaaatccga tccgcattcg
     4741 ccagggcgct tccgctgcaa cggcgtggtg atcaacgtgc cggagttcta cagcacgttc
     4801 gacgtgaagc cgggcgacaa gatgtacagc ccgccggaga agcgggtgaa gatctggtag
     4861 ctgcgggcgc acttgtgcgc tcgccgcgga aacgattaaa tccatcgcgt cggcggaggc
     4921 cagggagcct tcggtccgcc gacgtcttca attgaaaaaa agggggaacc atgtacaagc
     4981 attcgttgat gtcgatttcc gccgtcctcg cgctggcgct gatgacgggc tgcgcttcgg
     5041 taccaatggc aacaccggaa gccgacgccc aggccaagac gttcaaggcg ccggcggaca
     5101 aggccaacct ctacatctac cgcaacgaga gcttcggcag cgccgtcaag atgaccgtgc
     5161 tgctggacaa ccagctcgcc ggcgacacgg gtccgcatac ctacatcctg aagagcgtag
     5221 cgcccggttc gcacaccttg acctcgaaga ccgagaatga cgccacgctg accattgacg
     5281 ccgctgccgg caagaactat tacgtctggc aggaagtaaa gatgggcgcg ctctcggcgg
     5341 gttcgcaact gcacctggtt gacgatacgg ccggccaggc gggcgtgcag gattgcaagc
     5401 tggtgcaata acgctgcggt cgcgcggcct gtttcgccgg gccgatcagt ccagcactgg
     5461 cgccacgctg cgcggacgcc agaggaattt ctcgagattg acccggccgc gcaggaacac
     5521 gacgccttcc gcctcaagca gtttcttctg cgcgcgccag cgctcgctgc gcggcggaaa
     5581 cgagatgcgg ccctgcgcgt tgatcacgcg ctgccacggc agcttcaggc gcggcgggat
     5641 gttgaacatg gcataaccgg ccaggcgcgg ctgtttcggg taacccgcct cggccgcgac
     5701 ctggccgtag gtggccacgc ggccgcgcgg gattttccgc accacgtccc agatgcgctg
     5761 gtagctttcc cggtctgcca tgcgggctat tgtgcccgcg cggcgactac tcgtcctcgg
     5821 actcggccag ccgccgcagg cccgagggcg tcggcgcgat ggcaagctgg ccatcctgct
     5881 gggtgcaggt ggcgtagcgg cgctcgacca gttcgcgcac ggcgtcgcgc agttcgctgg
     5941 cacgcagacc gatgcggttc cattcctgtg caatcagcgc gaagcggatc ggcaggccgc
     6001 tgcccattcc tgcccgctgc accacatcca tgagtgccgc ttccagcctg cccgaagtcg
     6061 ccagcatgag aactctccaa cgcgtatcgc gatggagcca ttcaagcaga gcgcggagcg
     6121 tgcacagcta gagaaaaatc accgtcagcg acggcaggcg gcgcaggaca gatgaaaggt
     6181 ggttcagctt caggccggaa cggccatgcc catggaagcg ggcgtgtcgt gcagcgtggc
     6241 gctccaggtg gcgcggtagc cctcgcgctc gcgctcggac agccaggtgc ccagcgtggc
     6301 ctcggcatag gaacggccga acatcggctg caccttgcgc tggtggcgcc aggtgaggaa
     6361 ttcatcgttg gcgccttcgc acaggcgcag gtggaacgcg ccgtcggtcg cgcggctgag
     6421 aaacgcagca cagagtccgc gatggccgct ggcgtcggcc gggttgatca gcaggtggct
     6481 ggccagcagg gcttcatgct gctcgatcca ctcgctcagc gctgccagag aacccatgcc
     6541 cggaagaaat gaagttgacg ccatgaccct tttgcccaga ttcgatggcg ccaatatgac
     6601 aaaggggtca gctctccgct gtaggaaaag cctgagcccc cgcgaaggaa aggatcaggc
     6661 ggccgggcca gccgaccgcc catggctgcg tgaagcggcg atcctgtagg aagttagcca
     6721 gttgtacaac gaaatggcac gtgccccctc gtgccggcgc cagctcagcc gcgcttgtag
     6781 acgagctgct tcttgccgcc ttcgcaagtg cccaccacct tgccgtcctt gttctcgccg
     6841 gccggaacga tgtccagcgt gtatgccttc accccgtggc tcttgatctt gtcctcgacc
     6901 ttggccttga cctggtcgca cggcgtgccg gccaggcaga tgcccgttgc ggccagaatt
     6961 gcgagaccca accccatggt gatccgtttc atatcgcccc ctatccgtat ctatacataa
     7021 gcgtcaaatg cgcaacaagt ctgccagaag cgggccgtat cgaccattcg tcgcccgccc
     7081 gattgacgca cgcggcggcc gcttcgatta aggcgtcttc tcggggggat gtgaataact
     7141 tgcgccgtaa gctgtgcgcc atctgatcaa ctgccacgcc catgtacatc tcgcccaacg
     7201 acgttgaagc cgcgctgctg cgcatttatg ccgaactgca gattccgccc ggagccgcgc
     7261 tgagccacag ctacctgctc aagcgctggc gcgagaaccg gctgcgccac agcgatctcg
     7321 acgccacgct caaccggctg atccggtcgc ggcagatgga tgcgcgtgac agcttcgagg
     7381 gccgcgcctt tgcactgacg ccgtcaggcc agcggcgcgc gcgcaccttg cgcacaggcc
     7441 cgggcgggcg cctgctgcgc cggctgcgcc tggcgtgggc ccaggtgatg cgccggcgcg
     7501 gttcccgcga tggcgacgcc gcgcagcacc gccgcctgat cgaccgcgcg gccacccgca
     7561 tctgaaaccg ctcagagtgc cagccggcgc acgtccgaca gcagcttcac caggtgcacg
     7621 atgaagcgcg cggcataggc gccgtcgatc acgcgatgat cgtaggacag cgacagcggc
     7681 agcatcagcc gcggctggaa ttgcttgcca tcccacaccg gcttcatctg cgcacgtgac
     7741 gcaccgagga tggcgacctc cggcgcgttg atgatcggcg tgaagtggct gccgccgatg
     7801 ccgcccagcg acgagatgga gaagcagccg cccttcatct cgtcaggctt gagcttgccg
     7861 tcgcgcgcct tcttcgccag cgccccgcat tcttcggcca gctgcaccac gcctttcttg
     7921 tcgacctcgc gcaccaccgg caccaccagc ccgttctcgg tgtcggcggc aaagccgatg
     7981 tggcagtact ttttcaggac cagttcttcg ccaccgggcg ccagcgagct gttgaattcg
     8041 gggtaggtct gcatggcctt ggccaccgcc ttcatgatga agggcagcag cgtcagcttc
     8101 accccggttt cctccgccga cgatttgcgg aaggcttcga cctcggtgat gtcggcctcg
     8161 tcgaactgcg tcacgtgcgg caggttgagc caggcgcgcg acaggtgtgc cgccgacagc
     8221 ttgcggatgc gcgccagcgg ttttttttcg acggggccga actgcgagaa gtccaccgcg
     8281 ggaatcggcg ggatgccact gccgccgctt gcag
//
LOCUS       DASZZC010000767         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_187726,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000767 DASZZC010000000
VERSION     DASZZC010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187726"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..774
                     /gene="coq7"
                     /locus_tag="VHE37_16510"
     CDS             157..774
                     /gene="coq7"
                     /locus_tag="VHE37_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HVT37191.1"
                     /translation="MSRHYSAADRFLIGLHQRVAALARPAPAAPAEKNLDEAERRHAA
                     GLMRVNHAGEIAAQALYQGQAYTARDPHVREHLLKAAREEEKHLQWCEQRLAELGDAP
                     SALKPLWYAGSFAIGAAAGMAGDRWSLGFVAETEKQVSEHLQDHLQQLPPQDQRSREI
                     VAKMHEDETRHGREALEAGGRELPRPLRALMRQVARVMKFGAYRV"
     gene            complement(791..1336)
                     /locus_tag="VHE37_16515"
     CDS             complement(791..1336)
                     /locus_tag="VHE37_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVT37192.1"
                     /translation="MRNRLAAAIAALMLLYGGQAAANNGPDIAIGDALTGIVPLGALG
                     IAYFSGDTEGVHQWVRNTAINQTLISVLRLGFNQTSLGRRPNGGKYGFPSGHEAFVMS
                     GAAFLGERYTWELGLPAYLAAGYVGYVRVHEDKHHWRDVIASGAISYGIALVTVTPEH
                     ATHIAPIVGPNWLGLRFERSF"
     gene            complement(1635..1725)
                     /locus_tag="VHE37_16520"
     tRNA            complement(1635..1725)
                     /locus_tag="VHE37_16520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1689..1691),aa:Ser,seq:gct)
     gene            complement(1771..1847)
                     /locus_tag="VHE37_16525"
     tRNA            complement(1771..1847)
                     /locus_tag="VHE37_16525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1811..1813),aa:Arg,seq:acg)
     gene            complement(1929..2135)
                     /gene="csrA"
                     /locus_tag="VHE37_16530"
     CDS             complement(1929..2135)
                     /gene="csrA"
                     /locus_tag="VHE37_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HVT37193.1"
                     /translation="MLILTRRVGEAVMIGDEVCITVLDVKGSQVRLGVKAPRSVSVHR
                     EEIFERIKREGDTEDTAPSKQAAA"
     gene            complement(2206..3441)
                     /locus_tag="VHE37_16535"
     CDS             complement(2206..3441)
                     /locus_tag="VHE37_16535"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004771564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HVT37194.1"
                     /translation="MALIVQKYGGTSMGSVERIEHVANKVAGWRAQGHQIVVVVSAMS
                     GETDRLLKLAKSINPRGSAREFDQIAATGEQVTIGLLALALEKAGVPARSYTGPQVAI
                     KTDDAYMKARIQGIDADRLMADCKAGIVPVVAGFQGISPDGSTATLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRVEPKARRLDKITFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVPLRVVSTFVDGPGTLITLEDENVEQPLVSGIAFNRDEAQLTVAGVPDRPGIA
                     AAILGPIGDANIEVDMIVQNVGADGTTDFTFTVHRNDYERTLEITQKLCKELGAKGVK
                     GATDIVKVSLVGVGMRSHAGIASQMFKALAAAGINIRMISTSEIKISVVIDEMYLELA
                     VRTLHKAFGLENGKISTLK"
BASE COUNT          612 a         1148 c         1158 g          614 t
ORIGIN      
        1 atcagccctt catcaacttc atcgcgacgc aggtgaagcg gctcgatttt ggagcagccg
       61 acgtggcgcc cacgacggtg ccggaataca cgctcagctt tacgccgctg gaccagtgaa
      121 ccggcagcgg gccgcgatgc actaaaattg catcgcatga gccgccacta ttccgccgcc
      181 gaccgcttcc tcatcgggct gcaccagcgc gtcgctgcac tggcgcgccc ggcgccagcc
      241 gcgcccgcgg agaagaacct cgatgaagcc gagcggcgcc acgccgccgg gctgatgcgc
      301 gtcaaccacg ccggcgagat cgccgcgcag gcgctgtacc agggccaggc ctacaccgcg
      361 cgcgatccgc acgtgcgcga gcacctgctc aaggcggcgc gcgaggaaga gaagcacctg
      421 cagtggtgcg agcagcgcct ggccgaactg ggcgacgcgc ccagcgcgct caagccgctg
      481 tggtacgcgg gatcgttcgc catcggcgcg gccgcgggca tggccggtga ccgctggagc
      541 ctgggcttcg tcgccgaaac cgagaagcag gtatccgaac atctgcagga tcacctgcag
      601 cagctgccgc cgcaggatca gcgcagccgc gagatcgtgg cgaagatgca tgaagacgaa
      661 acgcgccatg gccgcgaagc gctggaagcc ggcggccgcg aactgccgcg cccgctgcgc
      721 gcgctgatga ggcaggtggc gcgcgtgatg aagttcggcg cgtaccgcgt ctagacgtgt
      781 ctggaagttt ctagaacgac cgctcgaagc gcagtcccag ccagttcggc ccgacgatgg
      841 gcgcgatgtg cgtggcgtgc tccggcgtca ccgtgaccag cgcgatgccg taggagatgg
      901 cgccgctggc gatcacgtcg cgccagtggt gcttgtcctc gtgcacgcgc acgtagccga
      961 catagcccgc cgcgagatac gccggcaggc ccagttccca ggtgtagcgc tcgccgagaa
     1021 acgcagcgcc ggacatgacg aacgcttcgt ggccggaagg aaagccgtac ttgccgccgt
     1081 tgggccggcg gcccagcgaa gtctggttga aacccaggcg cagcacgctg atcagcgtct
     1141 ggttgatcgc ggtgttgcgc acccactggt gcacgccctc ggtgtcgccg ctgaaatagg
     1201 cgatgcccag cgcacccagc ggcacgatgc cggtgagcgc atcgccgatg gcgatgtcgg
     1261 gcccgttgtt ggcggcagcc tgtccgccgt acagcagcat cagcgccgcg atggcggccg
     1321 ccagccggtt gcgcatgtcg tcattctcgt tctcgacttg cggatatgac tggcgcggac
     1381 ggcgcgcagt tcagccggca accatgtggt ggctaccgga accgggaatc cgatgaggtt
     1441 cgcatcgcgg gttcgaaccc tcgggcgacg taggagtttc aagaactggc ggagactgag
     1501 ggaaggcggc gtctgcgcgc cgccctgcgg gtatgcgtgc gcatacgccc gcttgcatgc
     1561 gcaagcgggt cgaacccgat gagtttcata tcgagggttc gaatccctct gggaacgcgg
     1621 ggagtttcaa gaactggcgg agactgaggg attcgaaccc tcgatgggct tttggcccat
     1681 acacccttag caggggtgcg ccttcgtcca ctcggccaag tctccgtgag cgggaagaac
     1741 ttactctaac agccaaagcc caacaaaaaa tggcgcgccc ggatggattc gaaccaccga
     1801 cccccaagtt cgtagcctgg tgctctatcc agctgagcta cgggcgcgtg ctgtgctgcc
     1861 gaccctcaag aggggcggca ttctaacggc agagtggagg gaggtaaaga aaaaaagcag
     1921 ccgcgcgctc aggcagcggc ttgcttgctc ggcgcggtgt cttcggtatc gccttcgcgc
     1981 ttgatgcgct cgaaaatttc ttcgcggtgc acggagacgc tgcgcggcgc cttcaccccg
     2041 agacggacct ggcttccctt cacgtccagc acggtgatgc acacctcgtc gccgatcatc
     2101 accgcctcgc cgacgcgcct ggtcaaaatc aacatggaac acactccttt gcgtgtgctg
     2161 acagtctcat cctttcgacc gggctgcaac agtcgattca actcactact tcagtgttga
     2221 aatcttgccg ttttccagac caaaggcctt gtgcagggtg cgaacggcga gctccaggta
     2281 catctcgtcg atcaccaccg agatcttgat ctccgaggtg gagatcatac ggatgttgat
     2341 gccggccgcc gccagggcct tgaacatctg gctggcgatg ccggcgtgcg agcgcatgcc
     2401 cacccccacc agcgacacct tgacgatgtc ggtggcgccc ttgacgccct tggcaccgag
     2461 ctccttgcac agcttctggg tgatctccag cgtgcgctcg taatcgttgc ggtgcacggt
     2521 gaaggtgaag tcggtggtgc catcggcacc gacgttctgc acgatcatgt cgacttctat
     2581 gttggcgtcg ccgatcggcc ccaggatggc ggcggcgatg ccgggccggt cgggcacgcc
     2641 ggcaacggtg agctgagcct catcgcggtt gaaggcgatg cctgaaacga gcggctgttc
     2701 cacgttctca tcctctagtg tgatcagggt gccggggccg tcgacaaagg tcgagaccac
     2761 gcgcagcggc accttgtact tgccggcgaa ttccaccgac ctgatttgca gcaccttgga
     2821 gcccaggctg gccatctcca gcatttcttc gaaggtgatc ttgtccagcc ggcgtgcctt
     2881 gggttccacg cgcggatcgg tggtgtagac gccgtctaca tcggtgtaga tctggcactc
     2941 gtcggccttc agcgccgccg ccagcgccac gccggtggtg tcagagccac cgcggccgag
     3001 cgtggccgtg ctgccatcgg gcgagatgcc ctggaagccc gccaccaccg gcacgatgcc
     3061 ggccttgcag tcggccatca gccggtccgc gtcgatgccc tggatgcgcg ccttcatgta
     3121 ggcgtcgtcg gtcttgatcg ccacctgcgg gccggtgtac gaacgcgccg gcacgcctgc
     3181 tttctccagc gccagcgcca gcaggccaat ggtgacctgc tcgccggttg cggcaatctg
     3241 gtcgaactcg cgtgcgctgc cgcgcggatt gatcgactta gccagcttga gcagccggtc
     3301 agtctcgccc gacatggcgg agaccacgac cacgatctgg tgaccctgcg cgcgccagcc
     3361 ggcgaccttg ttggccacat gctcgatgcg ctcgaccgaa cccatcgagg tgccgccgta
     3421 cttctgcaca atcagcgcca tggcgcgtca gacccgcttg gtgggttttt gggggcgcgt
     3481 attgtgggcg aagacggggc gggctgccac tgctgcaaac ggatggcgct cg
//
LOCUS       DASZZC010000768         7463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1282717,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000768 DASZZC010000000
VERSION     DASZZC010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282717"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..353
                     /locus_tag="VHE37_16540"
     CDS             93..353
                     /locus_tag="VHE37_16540"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HVT37195.1"
                     /translation="MNQTPPPEIAQRGPYMKEDLPPGTYLWCACGRTKTQPFCDGSHQ
                     GGPHQPLRFTVTPRSGTLWLCGCKRSRTPPFCDGAHNKLPRV"
     gene            433..840
                     /locus_tag="VHE37_16545"
     CDS             433..840
                     /locus_tag="VHE37_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37196.1"
                     /translation="MKKNTIACSIAVAMLGLSAPLFAADASVNANVNANLPAAQAQQM
                     KTDAQAQGAQMKDDAKAQGTEMKDAAKAQVGAAKDQVKSTATETGAQVKGGARKVKGV
                     AKNTAGALTGAVGGLTSGTQVQVDANAQAGDKK"
     gene            complement(905..1243)
                     /locus_tag="VHE37_16550"
     CDS             complement(905..1243)
                     /locus_tag="VHE37_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVT37197.1"
                     /translation="MKMLIAIIKPFKLDAVREALADIGVQGMTVTEVRGFGRQKGHTE
                     LYRGAEYTVDLLPKIKLEVALAPDMVDKAVEAISKAAKTGQIGDGKIFVYDLNQTVRI
                     RTGEAGVTAV"
     gene            1333..4389
                     /locus_tag="VHE37_16555"
     CDS             1333..4389
                     /locus_tag="VHE37_16555"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HVT37198.1"
                     /translation="MSQQPYKYWAFISYSHRDRAWGDWLHKALETYRVPRRLVGRESR
                     DGKIPARLFPVFRDRDELPSSADLGGVINQALADSRYQIVICSPRSAASRWVNEEVRH
                     FKSLGRADRVLCLIVDGEPNVFDVPGRDVEECFAPAVRFQVRGDGTITDEPAEPIAAD
                     AREHADGKAGAKLKLIAGLLGVGYDELAQRERQRRFWQRVQAAAAGMAFVAMVAGAWQ
                     WYLHQRDTRERQIVMEKLVENGRLELLDGNQARAAVYLNEAYKMGDDSAAVRFMLHQA
                     LKPVDALTNVRIKHDGYGALHGAAFSPDGDRFVLGAVSPDAGKHPSALVYDARTGQEL
                     ARLPDAPALPLVIKFFPDGRHLLLTGSTHETIADDLITRVWDIDTQKITLATKGANGI
                     AGEPLNQDGSQLLVADQSGLQVWNPSTGQILRTLIAGSAVSAATFSEDGRLIAAALGS
                     GKVVVLDAATGKTVSSLQEAFGQELSSIVFTPDGTRLLALSAQADVKVWETRSWRLAI
                     ALAADPSQVTALQFAQDGRRFVTVGSEGYKVWSTARGMLLFFQPRTTDWQGHGILSPD
                     GDTLIIVDASSKVAEAWDVRSKRKLYTLDLHAAGIAAAAYSSGGNSLLLTSRDGEAEI
                     WRMPVMPAWKYESFDTLPYVAKWDRSGKRVLMVGGGADNVGRGLLLDLATGKPLQTFD
                     GQKGILYGGAFNPAEDRVATASADGTVSLWNVKTGEKVSSLFQSAVGAYSASFNADGS
                     RVLTTSAPTASPEKDSAGLWNANDGHLIARLPHERSVFSAHFNNHGDRIVTTGADGAV
                     KLWDSRDGTLLRNLAAHHGWGVSASFSPDDRTMVTAGEDNSVKLWDGRSGAILKSLEN
                     PGLGVPTNAIFSPNASRIAIATQSGVIWIWSPSSGESLALKGLKQQVDGLRFSSDGNL
                     LFSSGDDGTARAWDVISGKELGVIASHDRQINSLELSPDGTRLLTNSWGQVELNDTSL
                     EVRGPGQLALLLQCQVPWSFDPVTQSIIAKVGEKGACGKLKFSTQK"
     gene            complement(4440..4955)
                     /locus_tag="VHE37_16560"
     CDS             complement(4440..4955)
                     /locus_tag="VHE37_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37199.1"
                     /translation="MKRILIAAATVVSLLGASAVVAGGLPLTHASAPIAAAKIVALPH
                     VATAAGLATSLGARAAQLPALPGLNSTEPAAGNYTWLDYDLHTYPGLLKSSVNYHISD
                     MNYRITYSTQLLTGCGSAEGFCNPVSYPTQFVLGAVSVLPGVLGDLGNGALGLIKGPA
                     PLPLPHMPGGL"
     gene            complement(5120..5776)
                     /locus_tag="VHE37_16565"
     CDS             complement(5120..5776)
                     /locus_tag="VHE37_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVT37200.1"
                     /translation="MTTPKFDRLSSDPAFQELFKASRHREFRKNQVVLQESAQPGFLY
                     LVVSGLLAVRHSGGRGRELLLAYFYPGDFFGEMCLFPGMEARSAMIKTQTASTVLEIG
                     YRQFLDLTAKHPSLWLELAGQLAARLRSTNHRLAEMPALHAADRVWLALKEIAAQSDA
                     PMLPEGKPVHITRQDLGKLAGCSRELAGMVLKDFAAEGRIKLRGKTVILPASEPAQSS
                     "
     gene            complement(5898..>7463)
                     /locus_tag="VHE37_16570"
     CDS             complement(5898..>7463)
                     /locus_tag="VHE37_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVT37201.1"
                     /translation="EKKKKKEKAAEAAKAEAAAAPAAAPAASAAAPEAAPAASAAAAP
                     AAAETASAAPAAAAPAADAAAAPAPTPKCGDKGFDCNKGDVSWMMTSTAFVLFMTVPG
                     LALFYAGMVRTKNALSTTMQSFSIFCVVAVLWVIFGYGMAFTAGDGPFIGAMPWSSAG
                     KGWFMGLDDPTTAVAATFSKGQYLPDFVYCMFQLTFAAITVALITGGLAERMKFSAVM
                     IFSVLWFVFSYLPIAHMVWYWDGPDAYTDAKAGDIATSHAGWLFQKGAIDFAGGTVVH
                     INAGIAALMSAIVLGPRKGLGKIHFAPHSLMMTAIGTGMLWMGWFGFNAGSALEANGG
                     AGLAFFNTLFGTAVAGCAWAGTEWILKGKPSLLGVCSGIVAGLVAITPAAGYVGPVGA
                     FVICAVAGVVCLLAATKLKALLGYDDALDAFGVHCIGGIIGALGTGIYVNPKLGGTGV
                     YDYVANKVGDFDASAQFVSQLWAVGTSLIWSGTVALVILLVLKYTIGIRATDEAQEEG
                     LDLADHGEKAYNM"
BASE COUNT         1416 a         2325 c         2406 g         1316 t
ORIGIN      
        1 cagcgaccac catgccatgc gtgtcatgaa aaactcccgc gatccggctt gaagcgcgcg
       61 gctattatgc acgccgcacc ggtgaacccc acatgaacca gacgccgccg cccgagatcg
      121 cccagcgcgg gccgtacatg aaggaagacc tgccgcccgg gacctacctg tggtgcgcct
      181 gcggccgtac gaagacgcag ccgttctgcg acggctcgca ccagggcggg ccgcaccagc
      241 cgctgcgttt caccgttacg ccgcgcagcg gcactctgtg gctgtgcggc tgcaagcgtt
      301 cgcgcacgcc gccgttttgc gacggtgcgc acaacaagct gccgcgcgtc tgaaccgttc
      361 aacaagggtt cagccagagc gcctagagtg cgtgccgcgt catccacctt ttccaccacg
      421 agggatagct ccatgaagaa gaacaccatt gcctgctcga tcgccgttgc catgctcgga
      481 ctgtccgcgc cgctgttcgc ggccgatgcg tcggtcaacg ccaacgtcaa cgccaacctg
      541 ccggcggcgc aggcgcagca gatgaagacc gatgcgcagg cgcagggcgc gcagatgaag
      601 gacgatgcca aggcccaggg caccgaaatg aaagatgccg ccaaggccca ggtcggcgcg
      661 gcgaaggacc aggtcaagag cactgccacc gagaccggcg cgcaggtcaa gggcggcgcc
      721 aggaaggtga agggtgtggc caagaacacc gccggcgcgc ttaccggcgc ggtgggcggg
      781 ctgacctccg gcacgcaggt gcaggtcgac gccaacgcgc aggccggcga caagaagtaa
      841 gtttcttccg caacaaacaa cgaaggccgc tcacgcggcc tttgtttttt atgtcgcctg
      901 aaagctagac cgcagtcacg cccgcctcgc cggtgcggat gcgcacggtc tggttgaggt
      961 cgtagacgaa gatcttgccg tcgccgatct ggccggtctt ggccgccttg ctgatcgcct
     1021 cgacggcctt gtccaccatg tccggcgcca gcgccacttc cagcttgatc ttgggcagca
     1081 ggtccaccgt gtactcggcg ccgcggtaca actcggtgtg gcccttctgc cggccgaagc
     1141 cgcgcacctc ggtcaccgtc atgccctgca cgccgatgtc ggccagcgcc tcgcgcacgg
     1201 cgtcgagctt gaacggcttg atgatggcga tcagcatttt catggtgcga ctccggaaat
     1261 ttagcccggg cagtttagcg cggccggtag ccggcgcggc aactgccgca taagcttgga
     1321 ccgcggggga gcatgtctca gcagccttac aagtattggg ccttcatcag ctacagccac
     1381 cgggatcgtg cctggggcga ctggctgcac aaggcactgg aaacctaccg cgtgccgcgg
     1441 cggctggtgg gccgtgagtc gcgcgacggc aaaattcccg cgcggctgtt cccggtgttc
     1501 cgcgaccgtg acgaactgcc gtcgtccgcc gatctcggtg gcgtgatcaa ccaggctctg
     1561 gccgactcgc gctaccagat cgtcatctgc tcaccgcgct cggcggcctc gcgctgggtc
     1621 aatgaagagg tgcggcactt caagtcgctg ggccgcgccg accgcgtgct gtgcctgatc
     1681 gtcgacggcg agcccaacgt gttcgacgtg ccggggcgcg atgtcgagga atgcttcgca
     1741 ccggcggtgc gcttccaggt gcgcggcgac ggcaccatca ccgacgagcc tgccgagccc
     1801 atcgctgccg atgcgcgcga gcacgccgac ggcaaggccg gggcaaaact gaaactgatc
     1861 gccggcctgc tcggcgtcgg ttacgacgaa ctggcgcagc gcgagcgcca gcgccgcttc
     1921 tggcagcggg tacaggcggc agctgccggc atggccttcg tcgccatggt cgcaggtgca
     1981 tggcagtggt acctgcacca gcgcgatacg cgcgagcggc agatcgtgat ggaaaaactg
     2041 gttgagaacg gccggctgga attgctcgat ggcaaccagg cgcgcgccgc ggtgtatctc
     2101 aacgaagcct acaagatggg cgacgactcg gccgcggtgc gcttcatgct gcaccaggcg
     2161 ctgaagccgg ttgatgcgct gacgaacgtg cggattaagc acgatggata tggagcgctg
     2221 cacggcgccg cgttcagtcc ggacggcgac aggttcgtcc tgggcgcggt atccccagac
     2281 gccggcaagc acccaagcgc actggtttac gacgcgcgga cggggcagga gcttgcccgc
     2341 ctgccggatg ctccggcctt gccgctggtg atcaagtttt tccccgacgg ccgtcatttg
     2401 ttgctgacag gctcgacaca cgagaccatc gccgacgatt tgatcacccg cgtttgggat
     2461 attgataccc aaaaaatcac gctcgcaacc aagggcgcga atggcatcgc gggcgagccg
     2521 ttgaaccagg atggttcgca gctgctggtc gcggatcaat cgggcctgca ggtgtggaat
     2581 ccatcgactg gccaaatcct gagaacgctt atcgccggca gtgcggtttc tgccgccact
     2641 ttcagtgaag acgggcggct gattgctgcg gctctcggta gcgggaaagt agtggtgctg
     2701 gatgccgcca ccgggaaaac cgtttcaagc ttgcaggagg catttggaca agagctgtcg
     2761 agcatagtgt tcacgcccga cggtactcgc ctgcttgcgt tgtctgcaca agcggacgtc
     2821 aaggtttggg aaacccgcag ctggcgcctg gctatcgcat tggcggccga tccgtcgcaa
     2881 gtgactgctc tccagttcgc ccaggacggc cgccgctttg tcactgttgg ttcagagggc
     2941 tacaaggtct ggagcacggc gcgcggaatg ttgcttttct ttcagccacg aacgacggac
     3001 tggcagggcc acggcatcct gagcccggac ggcgatacgt taataatcgt cgatgcgtcc
     3061 agtaaggtgg ccgaagcctg ggatgtgcgt tcaaagcgca agctctacac actcgatctg
     3121 catgcagccg gcattgcggc ggctgcgtat tcgtctggcg gcaatagcct gctgctgacc
     3181 agtcgtgacg gcgaagcaga aatctggcgt atgccggtca tgccggcatg gaagtacgaa
     3241 tcgtttgaca cgctgcccta tgtcgcgaag tgggatcggt ccgggaagcg ggtgctcatg
     3301 gtgggaggcg gagccgacaa tgtcggccgc ggcctcttgc tcgacctggc gactggcaag
     3361 cctctgcaga cgttcgatgg ccaaaaggga attctctacg gcggtgcgtt caatcctgct
     3421 gaagatcgag tggctaccgc cagtgccgat ggcactgttt cgctgtggaa tgtaaagaca
     3481 ggcgaaaagg tttcgtcgct ttttcagtcg gccgtcggag cctattccgc aagcttcaac
     3541 gccgatggca gccgggtgct gacgacctcg gcacctactg catcgcctga aaaagactct
     3601 gccggtttgt ggaacgccaa cgatggccat ttgattgcac ggcttccaca cgagcgcagc
     3661 gtgttctctg cacatttcaa caaccatggc gatcgtatcg ttaccaccgg cgcagacggg
     3721 gcagtcaagc tatgggatag ccgcgatggc acgcttttgc gcaatttggc tgcgcatcat
     3781 gggtggggag tgtcagccag tttcagcccg gacgaccgga caatggttac ggcaggtgaa
     3841 gacaacagcg tcaagctctg ggatggcaga tccggcgcaa tacttaaaag cctggagaac
     3901 ccggggctgg gtgttcccac gaacgccatt ttttccccca atgccagccg catcgcaatc
     3961 gccacgcaat ctggcgtgat ctggatctgg tctccaagca gcggcgagtc gctcgcactt
     4021 aaaggcttaa agcagcaggt agatggcctg cgcttttcct cggacgggaa tttgttattc
     4081 agctcgggag atgatggtac ggcccgggcc tgggacgtca tctccggcaa agaactgggg
     4141 gtgattgcgt cgcacgaccg gcagatcaat tcgcttgagc tgtcgcccga tggcacccgc
     4201 ttgctcacca atagctgggg ccaggtcgaa ctgaatgaca cgtcgctgga agtccgcggt
     4261 ccgggacagc ttgccttgct gctccagtgc caggtcccct ggagtttcga tcctgtaacg
     4321 cagagcatca ttgccaaggt tggagaaaag ggggcttgcg ggaagctgaa gttttccacg
     4381 cagaaataag aaggcccgga cgcttattca gcggccgggc cttgcctata tctaatattt
     4441 tacaacccgc ccggcatgtg aggcagcggc agcggcgccg gacctttgat cagcccaagt
     4501 gcgccgttcc ccagatcacc cagtactcca ggcagcacgc taaccgcacc cagcacaaac
     4561 tgagtcggat aactcaccgg attgcagaag ccttccgcgg atccgcaacc cgtgagcagc
     4621 tgggtgctat aggtgatccg gtagttcatg tcgctgatgt gatagttcac cgaggacttg
     4681 agcagccccg gatacgtgtg caggtcgtag tcgagccagg tgtagttgcc cgccgccggc
     4741 tccgtcgagt tcaagccggg cagcgctggt agctgcgcgg ctctggcgcc gagcgatgtg
     4801 gccaagccgg ccgctgttgc gacgtgaggc agcgctacga tctttgctgc agcgatgggc
     4861 gcactcgcat gagtcagcgg caggccgccc gctaccaccg ctgaagcacc gagcagcgag
     4921 accacagtgg ctgcggcaat cagaattctc ttcatgtaca gatctcccgt taactgtgat
     4981 gcggagcgga agagacaagt cgtcactcgc tccatgcccc ctagaagacc atttgctcgc
     5041 cattcggtca acgaacgaat gtgaactggg cgacaaatgt cctgtaggaa atttgcccgg
     5101 acgccagctg ttgccgcatt caagaggatt gcgcgggctc tgaagcggga aggatgacag
     5161 ttttgccgcg cagtttgatg cggccttcgg cagcaaagtc cttgagcacc atgccggcca
     5221 gttcacggct gcagccggcg agcttgccga gatcctggcg ggtgatgtgc accggcttgc
     5281 cttcgggcaa catcggcgcg tcactctgcg ccgcgatctc cttcagcgca agccacacgc
     5341 gatcggcggc gtgcagcgcg ggcatttccg ccaggcggtg attggtcgag cgcagccgcg
     5401 cggcgagctg gccggccagc tcgagccaca ggctcggatg tttggcggtg agatcgagaa
     5461 attgccggta gccgatttcc agcacggtgg atgccgtttg tgtcttgatc atcgcgctgc
     5521 gcgcttccat gccggggaac aggcacatct cgccgaagaa atcgcccggg taaaaatagg
     5581 ccaggagcaa ttcgcggccg cggccgccgg aatgtctcac cgccagcaaa cccgacacga
     5641 cgaggtagag aaatccgggc tgggcggatt cctgcagcac cacctggttc ttgcggaact
     5701 cgcggtggcg cgaggccttg aacaattcct ggaaagccgg atcgctgctc aggcgatcga
     5761 atttcggcgt ggtcatcctg actccctgca caggtccgca gcgcaggcag gataaccgaa
     5821 tccgtccgcg caaaagaaaa ggggcggcct tgcggccgcc cccggaaaac ccgctgactg
     5881 gcagcaggag gagatgttca catgttgtag gccttctcgc cgtgatcggc cagatccagg
     5941 ccttcttcct gcgcctcgtc ggtcgcgcgg atgccgatgg tgtacttgag caccagcagg
     6001 atgaccagcg ccaccgtgcc agaccagatc agcgaagtgc cgacggccca cagctggctg
     6061 acgaactgcg cgctggcgtc gaaatcgccg accttgttgg ccacgtagtc gtacacgccg
     6121 gtgccgccca gcttcgggtt gacgtagatg ccggtgccga gcgcgccaat gatgccgccg
     6181 atgcagtgca cgccgaacgc gtcgagtgcg tcgtcataac cgagcagcgc cttcagcttg
     6241 gttgcggcca gcaggcagac cacaccggcc accgcgcaga tcacgaatgc gcccacggga
     6301 cccacgtagc cggctgccgg cgtaatcgcc accaggcccg ccacgatgcc ggagcacaca
     6361 ccgagcaggc tcggcttgcc cttgaggatc cactcggtgc ccgcccatgc gcagccggcg
     6421 acagcagtgc cgaacagcgt gttgaagaag gccaggccgg cgccgccgtt ggcttccaga
     6481 gccgagccgg cgttgaagcc gaaccagccc atccacagca tgccggtgcc gatggccgtc
     6541 atcatcaggc tgtgtggggc aaagtggatc ttgccaaggc ccttgcgcgg gccgagcacg
     6601 atggctgaca tcagcgcggc gataccggcg ttgatgtgca ccacggtgcc gccggcgaag
     6661 tctatggcgc ccttctggaa cagccagccc gcgtgcgagg tggcaatgtc acccgccttg
     6721 gcgtccgtgt acgcgtcggg gccgtcccag taccagacca tgtgcgcgat cggcaggtac
     6781 gaaaagacga accagaggac gctgaagatc atgaccgccg agaacttcat gcgctcagcc
     6841 aggccgccgg tgatcagcgc aacggtaatg gcggcgaaag tcagctggaa catgcagtag
     6901 acgaaatccg gcaggtactg gcccttgctg aaggtcgccg ccaccgcggt cgtcggatca
     6961 tccaggccca tgaaccagcc cttgccggcc gagctccacg gcatcgcgcc gatgaagggg
     7021 ccgtcgcctg ccgtaaaggc catgccatag ccgaagatga cccagagcac agcgaccacg
     7081 cagaagatgg agaagctctg cattgtcgtg gacaaggcgt tcttggtgcg caccatgccc
     7141 gcgtagaaca gcgcgagacc gggcaccgtc atgaacagca cgaaggcggt ggatgtcatc
     7201 atccacgaca cgtcgccctt gttgcagtca aagcccttgt cgccgcattt gggcgtcggt
     7261 gcgggagcgg ccgcagcatc cgcagcgggc gcagctgctg ccggcgcagc cgaagcggtt
     7321 tcagccgcag ccggagctgc cgctgccgaa gcggcgggcg cggcctcggg cgcggcggcg
     7381 gatgctgcag gtgcagctgc cggagcggct gctgcttccg ccttggcggc ttcagctgcc
     7441 ttttccttct tcttcttttt ttc
//
LOCUS       DASZZC010000769         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_759385,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000769 DASZZC010000000
VERSION     DASZZC010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759385"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /gene="ligD"
                     /locus_tag="VHE37_16575"
     CDS             <1..1901
                     /gene="ligD"
                     /locus_tag="VHE37_16575"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HVT37202.1"
                     /translation="EKKSAPATARARRAPAKMKRAPAMPALDELEPQLATLVDAAPGD
                     EAGYVYEIKFDGYRVLASIDGGKARLRSRNQLDWTGTFATVARELEKLKCSDAILDGE
                     ICYVQDDGRTDFQHLQNVLHPQGARVDHRRIVYYVFDLLWLDGQDLRPLPLVQRKAQL
                     TRLLGKRKGVVRFSEHLAGKVQDIFGHACRLGLEGVIGKRLDAPYREGRGRDWIKLKC
                     SQRQEFVIVGYSAPGGSRSGFGALLLASKEHGRLRYNGKVGTGFNEASLKDLSRRLMK
                     LKIDKPAVEGAPRMRNVSWVRPELVCEVAYTEMTRDGSLRHPSFQGLREDKPAAAVTR
                     EEAAPVAKAKKKTGSGKDSAAVGGVRITHPERVMDEQSGITKLQLARYHERVGELFLR
                     YAAQRPLALVRCPEGAAAQCFFQKHVTRGVGKKVQQAKFDAVLVDDLEGVLQLVQYNV
                     VEFHGWGARMPKVEKPDWIIFDLDPDTGLDFGHVVEAAFEVRDALAKIGLKTAVKTTG
                     GKGLHVVAPIVPRAGWDEVKEFSHRIALALEQQKPQRYVANMAKARRKGKIFVDYLRN
                     GRGATGVLPYSPRARPGMHVAMPLDWKELEAIKPGDFSVANVDDWIGRKDPWARFEGL
                     RSELPRRG"
     gene            1958..2974
                     /locus_tag="VHE37_16580"
     CDS             1958..2974
                     /locus_tag="VHE37_16580"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HVT37203.1"
                     /translation="MNLKILNETAKAMVAPGRGLLAADESTGTIKKRFDKIGVENTEP
                     NRQAYRDMLFTTPGFEQHTSGVILFEETLFQKGLDGTPFPKLLESKGVIPGIKVDAGA
                     KPLPFGMPDETITEGLDGLRERLQKYKAAGARFAKWRAVIVIGNGAPSYTAISTNAHA
                     LARYAALCQEADIVPIVEPEVLMDGDNTAQVCEDVTSWMLQETFTQLSYQKVALEGIV
                     LKPNMIVSGIKCATQAGVQEVAERTLKVLKRFVPPAVPGIAFLSGGQSDELATAHLDA
                     MNKAGNLPWKLTFSYGRALQAAALKAWGGKNVEAGKKAHLHRAKMNGLAALGKWDKSL
                     EKAA"
     gene            3050..>3793
                     /locus_tag="VHE37_16585"
     CDS             3050..>3793
                     /locus_tag="VHE37_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT37204.1"
                     /translation="MTKLADKVVWITGASSGIGEMLAYGAAERGARIVLTSRRQAELE
                     RVRNACADPARVGVLPADLTAFDAKELAQKAAGFFGPVDVLVNNAGWSQRSLARDTDM
                     AVYRRLMELDFFAPVALTQAVLPGMLASEHGGHVVMVGSVVSRFGAPLRSGYSAAKHA
                     LAGFTEAARTELWREQVKFTLVCPGFVKTQVSVNALDGHGGTHGRMDGGVEKGMDARA
                     CAEKIWRAVERDVEETLIGAQAKFVHLKRF"
BASE COUNT          691 a         1220 c         1342 g          540 t
ORIGIN      
        1 ccgagaaaaa atccgcgccg gccaccgcgc gtgcgcggcg cgcgcctgcg aaaatgaaac
       61 gcgcgccggc gatgcccgcc ctcgatgagc tggaaccgca gctggcgacg ctggtcgatg
      121 ccgcgccggg cgacgaggcc gggtacgtct acgagatcaa gttcgacggc taccgcgtgc
      181 tcgcttcaat cgatggcggc aaggcgcgcc tgcgctcgcg caaccagctg gactggaccg
      241 gcaccttcgc caccgtggcg cgcgagctgg aaaagctgaa gtgcagcgac gccatactcg
      301 atggcgaaat ctgctacgtg caggacgacg gccgcaccga tttccagcac ctgcagaacg
      361 tgctgcaccc gcagggcgcg cgggtcgatc accgtcgcat cgtctactac gtgttcgacc
      421 tgctgtggct cgacggccag gacctgcgac cgctgccgct ggtccagcgc aaggcgcaac
      481 tgacccggct gctgggcaag cgcaagggcg tggtgcggtt ttccgagcac ctcgccggca
      541 aggtgcagga catcttcggc cacgcctgca ggctcgggct ggaaggtgtg atcggcaagc
      601 ggctggatgc gccgtaccgc gaaggccgcg gccgcgactg gatcaagctg aagtgcagcc
      661 agcggcagga attcgtcatc gtcggctata gcgcgcccgg cggttcgcgc agcggcttcg
      721 gcgcgctgct gctggcgtcg aaggagcatg gcaggctgcg ctacaacggc aaggtcggca
      781 ccggcttcaa cgaagcctcg ctgaaagacc tgtcgcggcg gctgatgaag ctgaagatag
      841 acaagccagc agtcgagggc gcgccgcgca tgcgcaacgt cagctgggtg cggccggaac
      901 tggtgtgcga ggtggcctac accgagatga cacgcgacgg ttcgctgcgc cacccttcgt
      961 tccagggttt gcgcgaggac aagccggcag cggcggtcac gcgcgaggag gcggcgccgg
     1021 tggcaaaagc gaagaaaaaa acgggcagcg gcaaggacag cgccgccgtt ggcggcgtgc
     1081 gcatcacgca tcccgagcgc gtgatggatg agcagtccgg catcaccaag ctgcagctcg
     1141 cgcgttacca cgagcgcgtg ggcgaactgt tcctgcgcta cgcggcgcag cggccgctgg
     1201 cgctggtgcg ctgccccgaa ggggcggcgg cgcaatgctt cttccagaaa cacgtgacgc
     1261 gcggcgttgg caagaaggtg cagcaggcga aattcgacgc ggtgctggtc gacgacctgg
     1321 aaggcgtgct gcagctggtg cagtacaacg tcgtcgagtt ccacggctgg ggcgcgcgca
     1381 tgccgaaagt cgagaagccc gactggatca tctttgatct cgaccctgac acgggcctgg
     1441 atttcggcca cgtggtcgaa gcggcattcg aggtgcgcga tgcgctggcg aagatcggcc
     1501 tgaagaccgc ggtgaagacc accggcggca agggcctgca cgtggtggcg ccgatcgtgc
     1561 cgcgtgccgg ctgggacgag gtgaaggagt tttcccaccg gattgcgctg gcgctggagc
     1621 agcagaagcc gcagcgctat gtcgccaaca tggccaaggc caggcgcaag ggaaagatct
     1681 tcgtcgacta cctgcgcaac gggcgcggcg ccaccggcgt gctgccgtac tcgccgcgcg
     1741 cgcggccggg catgcacgta gccatgccgc tggactggaa ggaactggaa gcaatcaagc
     1801 ctggcgattt ctccgtggcc aatgttgacg actggatcgg acgcaaggat ccgtgggctc
     1861 gcttcgaggg cctgcgcagc gagctgccgc ggcgtggttg atataatcgc cgccccgcgc
     1921 agcgcgcggt ccagacatcc gaacgagagc gagcatcatg aatctgaaga tcctcaacga
     1981 gacggccaag gccatggtcg ccccgggccg cggcctgctg gcagcggacg agtccaccgg
     2041 caccatcaag aagcgcttcg acaagatcgg cgtggaaaac accgagccca accgccaggc
     2101 gtaccgcgac atgctgttca ccacgcccgg cttcgagcag cacaccagcg gcgtgatcct
     2161 gttcgaggag acgctgttcc agaagggtct cgatggcacg ccgttcccca agctgctgga
     2221 aagcaagggc gtgatccccg gcatcaaggt cgacgccggc gccaagccgc tgccgttcgg
     2281 catgcccgat gaaaccatca ccgaaggcct ggatggcctg cgcgagcgcc tgcagaagta
     2341 caaggcggcg ggtgcgcgct ttgccaagtg gcgcgcggtc atcgtcatcg gcaacggcgc
     2401 gccgagctac accgccatca gcaccaacgc gcatgcgctg gcgcgctacg cggcgctgtg
     2461 ccaggaagcg gacatcgtgc ccatcgtcga gccggaagtg ctgatggacg gcgacaacac
     2521 cgcgcaggtg tgcgaggacg tgacctcgtg gatgctgcag gaaaccttca cccagctgtc
     2581 gtaccagaag gtggcgctgg aaggcatcgt gctcaagccc aacatgatcg tgtccggcat
     2641 caagtgcgcc acgcaggccg gcgtgcagga agtcgccgag cgcacgctga aggtcctgaa
     2701 gcgtttcgtg ccgcccgccg tgccgggtat cgccttcctg tccggcggcc agagcgacga
     2761 gctggccacc gcgcacctgg atgccatgaa caaggccggc aacctgccgt ggaagctgac
     2821 cttctcctac ggccgcgccc tgcaggcggc ggcgctcaag gcctggggcg gcaagaacgt
     2881 ggaagcgggc aagaaggcgc acctgcaccg cgcgaagatg aacggcctgg cggcgctggg
     2941 caagtgggac aagtcgctcg agaaggcggc ctgatttttt ccttcccatc cggccgctcg
     3001 tgcgcaggcg cgagcggcct ttttgttgca ccgacgacaa ggaaagacga tgaccaaact
     3061 cgcagacaag gtggtctgga tcaccggcgc ctccagcggc atcggcgaaa tgctggccta
     3121 cggcgcggcc gagcgcggcg cgcgcatcgt gctcacctcg cggcgccagg cggaactcga
     3181 gcgcgtgcgc aacgcctgcg ccgaccccgc acgcgtgggc gtgctgccag ccgatctcac
     3241 cgcgttcgac gcgaaggagc tggcgcagaa ggccgcaggt ttcttcgggc cggtcgatgt
     3301 gctggtgaac aacgccggct ggtcgcagcg cagcctggcg cgcgacaccg acatggcggt
     3361 gtaccgccgg ctgatggagc tcgacttctt cgcgccggtg gcgctgaccc aggcggtgct
     3421 gccgggcatg ctggccagcg agcacggcgg acacgtggtc atggtcggca gcgtggtgtc
     3481 gcgcttcggc gcgccgctgc gcagcggcta ctccgcggcc aagcacgcgc tggccggctt
     3541 caccgaggcg gcgcgcaccg aactgtggcg cgagcaggtc aagttcacgc tggtctgccc
     3601 gggcttcgtc aagacgcagg tttcggtcaa cgcgctcgac ggccacggcg gcacgcacgg
     3661 acgcatggac ggcggcgtgg agaaaggcat ggacgcgcgc gcctgcgcgg agaagatctg
     3721 gcgcgcggtg gagcgcgacg tggaagaaac gctgatcggc gcgcaggcca agttcgtcca
     3781 cctcaagcgc ttc
//
LOCUS       DASZZC010000770         5161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_188485,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000770 DASZZC010000000
VERSION     DASZZC010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188485"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VHE37_16590"
     CDS             complement(<1..313)
                     /locus_tag="VHE37_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37205.1"
                     /translation="MIATAASAQAQEAGTDSAAALAAPEAAAVPAADVSAAADQAPAQ
                     DAGAAAQTAPAIAVNPLDAPAVTAAAASADEAPQELPAVIVTSQKFSQTLEVVPASVS
                     TV"
     gene            complement(456..3053)
                     /gene="clpB"
                     /locus_tag="VHE37_16595"
     CDS             complement(456..3053)
                     /gene="clpB"
                     /locus_tag="VHE37_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HVT37206.1"
                     /translation="MRMDKLTSRFQQALADAQSLAVGRDHPAIEPLHLMSALLNQDGG
                     STLPLLQAGGVNVDLLRSKLAQAFERLPRLGEATGDVNASSDLAKLLNLTDKLAQKRG
                     DQFISTELFVLAACDDKGALGEALRAAGARKELLEKAIEQVRGGQKVNDANAEEQRGA
                     LEKYTIDLTARAQSNKLDPVIGRDEEIRRVIQVLSRRTKNNPALIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLKNKRLLTLDLGALIAGAKFRGEFEERLKAVLTDLAKHEGNVILF
                     IDEIHTLVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQ
                     KVFVGEPSVEDTIAILRGLKERYEVHHGVDITDGALIAAAKLSHRYITDRNLPDKAID
                     LVDEAASRIRIEIDSKPEALDRLHRRLIQLKIEREALKKEHDEGAKKSLKALEADIAK
                     LEREYSDLEEKWKAEKASVQGSAGVKEELERARMDMDAARRAGDLARVAELQYGKIPE
                     LEKRAAAAAASGKGDAPKKFELLRNSVSEDEIAEIVARWTGIPVSKLMEGERDKLLRM
                     EAALHERVVGQDEAVSAVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELTKALAN
                     FLFDSEDAMVRIDMSEYGERHSVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDE
                     VEKAHPEVFNVLLQVLDDGRLTDGQGRTVDFRNAVLVMTSNLGSQLIQDMLGKDKKFD
                     YAAIKEAVMTVVGTHFRPEFINRIDEAVVFHPLGSAQIRAIAKVQGQRVIKRLAERNI
                     GLEFSDAALDKLAEAGYDPVYGARPLKRAIQTLVENPLARKVLDGEFAQGDRVRIDAD
                     ARGLSFSKA"
     gene            3307..3912
                     /locus_tag="VHE37_16600"
     CDS             3307..3912
                     /locus_tag="VHE37_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HVT37207.1"
                     /translation="MAGASHAAITKMVTDSIEVKQKLLADRALLDAIARVCDVCVDAL
                     KRGNKIIFAGNGGSAADSQHLAAEFVARFEFDRPGLASISLTVDTSALTAIGNDYGYE
                     FVFSRQLESNGKSGDVFFGLSTSGNSKNIIKAVEQAKKIGVTTVALCGAGGKLKDMCD
                     HALCAPSTHTPRIQECHGLIGHIICALCEELMYPEYKKPKV"
     gene            4063..4641
                     /locus_tag="VHE37_16605"
     CDS             4063..4641
                     /locus_tag="VHE37_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVT37208.1"
                     /translation="METIPAILKLDAGGLPVSWIRWQTAVTLYARDRVRWEAGDGRFT
                     IHGGICARTGLQSRIEIGSIIAVADKSKRFEKGVPLLTNRTLFQRDRNLCLYCGEQFP
                     VSYLTRDHVMPASRGGQSVWENCVTACRDCNQRKDDRTPDEANMKLLAVPYTPNLAEY
                     LILQNRRILADQMDFLLAYARKHGHHGGRHAA"
     gene            complement(4651..>5161)
                     /locus_tag="VHE37_16610"
     CDS             complement(4651..>5161)
                     /locus_tag="VHE37_16610"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT37209.1"
                     /translation="LRRTDVHQMAEALSAVADHVGPVYGVIAHSLGAAAAVQLLADGR
                     HAAARLVLIAPGGELAADIRRLAQALDLPPRCVAALVAALERHYDMPVEMCSISRLAR
                     MLNVAALVVHDVDDKVIPFAEGAQVARSLPGARFHATERLGHRRILRDAAVIGSVVEF
                     CRARRARTA"
BASE COUNT          809 a         1741 c         1701 g          910 t
ORIGIN      
        1 ccaccgtgct gaccgaggcc ggcaccactt ccagcgtctg gctgaatttc tgcgaggtga
       61 cgatgaccgc gggcagttcc tgcggcgctt cgtccgccga cgcggcggct gcggttacgg
      121 cgggcgcatc gagcgggttc acggcaatgg ccggtgcagt ttgggctgcg gcaccggcat
      181 cctgcgccgg cgcttgatct gccgccgccg acacatctgc ggcgggaacc gccgcagcct
      241 cgggagcggc cagcgctgcc gcggaatcgg tgccggcttc ctgcgcctgc gcggaagccg
      301 cagtcgcaat catcaatagc agcgccccag tgggcgccgg caggcgtcga accatgatcc
      361 atcctcaacg gaaacagcgc cgcgccccca aagcgcgcac gtcaaggcgc tattttgagg
      421 catccgccgc ggatgtgaca gcgggatgac gcgcgtcagg ccttgctgaa gctcagcccg
      481 cgcgcatcgg cgtcgatgcg cacgcggtcg ccctgcgcga actcgccgtc gagcaccttg
      541 cgcgccagtg gattttccac cagcgtctgg atcgcgcgct tgagcggccg cgcgccgtac
      601 accgggtcgt agcccgcctc cgccagcttg tcgagcgcgg catccgagaa ctcaagtcca
      661 atgttgcgct cggccaggcg cttgatcacg cgctggccct gcaccttggc gatggcgcgg
      721 atctgcgcgc tgcccagcgg gtgaaacacc acggcctcgt cgatgcggtt gatgaactcc
      781 gggcggaaat gcgtgccgac cacggtcatc accgcctcct tgatggcggc gtagtcgaac
      841 ttcttgtcct tgccgagcat gtcctgaatc agctgcgagc cgaggttcga cgtcatcacc
      901 agcacggcgt tgcggaaatc gaccgtgcgg ccctggccat cggtcaggcg gccgtcgtcc
      961 agcacctgca gcagcacgtt gaacacttcg ggatgggcct tttcgacctc gtcgagcagg
     1021 atcacggagt agggacggcg gcgcacggct tcggtcagct ggccgccttc ctcgtagccg
     1081 acgtagcccg gcggcgcgcc gatcagccgc gccacgctgt ggcgctcgcc gtactcgctc
     1141 atgtcgatgc gcaccatggc gtcctcgctg tcgaacagga agttcgccag cgccttggtc
     1201 agctcggtct tgccgacgcc ggtcgggccg aggaacagga acgagccgat cggccggttg
     1261 ggatctgaca agcccgcgcg cgagcggcgg atggcgttgg ccaccgcgct cacggcctcg
     1321 tcctggccga ccacgcgctc gtgcagcgcc gcttccatgc gcagcagctt gtcgcgctcg
     1381 ccttccatca gtttcgaaac cgggatgccg gtccagcgcg cgacgatctc ggcgatctca
     1441 tcctcgctca cgctgttgcg cagcagctcg aatttcttcg gcgcatcacc cttgccgctg
     1501 gcggcggccg cggcagcgcg cttttccagc tcgggaattt ttccgtactg cagttctgcc
     1561 acgcgtgcca ggtcgcccgc gcgacgcgcc gcgtccatgt ccatgcgcgc gcgttcgagt
     1621 tcttccttga cgccggcgct gccctgcacg ctggccttct cggctttcca tttctcttca
     1681 aggtcgctgt actcgcgttc gagcttggcg atgtcggctt ccagcgcctt cagcgatttc
     1741 ttcgcgccct cgtcatgctc cttcttcagc gcctcgcgct cgatcttcag ctggatcagg
     1801 cggcgatgaa gacgatccag cgcctcgggc ttggagtcga tctcgatgcg gatgcggctc
     1861 gccgcctcgt cgaccaggtc gatggccttg tcgggaagat tcctgtcggt gatgtagcgg
     1921 tgcgacagct tggccgcggc gatcagcgcg ccgtcggtga tgtcgacgcc gtgatgcacc
     1981 tcgtagcgtt ccttgaggcc gcgcaggatg gcgatggtgt cctccacgct cggctcgccg
     2041 acgaacacct tctggaagcg gcgctcgagc gcggcgtcct tctcgatgta tttgcggtat
     2101 tcatccagtg tggtggcgcc gatgcaatgc aactcgccgc gcgccagcgc cggcttgagc
     2161 atgttgccgg catccatggc gccctcggcc ttgccggcgc cgaccagcgt gtggatctcg
     2221 tcgatgaaca ggatcacgtt gccttcgtgc ttggccaggt cggtgagcac ggccttgagg
     2281 cgctcctcga actcgccgcg gaacttggcg ccggcgatca gcgcgccgag gtcgagcgtg
     2341 agcaggcgct tgttcttcag cgactccggc acctcgccgt tgacgatgcg ctgcgccagc
     2401 ccttcgacga tggcggtctt gcccacgccg ggttcgccga tcagcgcggg gttgttcttg
     2461 gtgcggcgag acagcacctg gatgacccgg cggatttcct cgtcgcggcc gatcaccggg
     2521 tcgagcttgt tcgactgcgc gcgcgcggtg aggtcgatgg tgtatttctc cagcgcgccg
     2581 cgctgctcct cggcattggc gtcgttcacc ttctgcccgc cgcgcacctg ctcgatggct
     2641 ttctccagga gttccttgcg cgcgcccgcg gcgcgcagcg cctcgcccag cgcgcccttg
     2701 tcgtcgcagg cggcgagcac gaacagttcg gtggagatga actggtcgcc gcgcttctgc
     2761 gccagcttgt cggtgagatt gagcagcttg gccaggtcgc tgctggcatt gacgtcgccg
     2821 gtggcctcgc ccaggcgggg caggcgctcg aaggcctgcg ccagcttgct gcgcagcagg
     2881 tcgacgttga cgccgccggc ctgcagcagc ggcagcgtgg agccgccgtc ctggttgagc
     2941 agcgcgctca tcaggtgcag cggctcgatg gccgggtgat cgcggcccac ggccaggctt
     3001 tgcgcgtcgg cgagcgcctg ctggaaacgg ctggtcagtt tgtccatgcg cacggcaatg
     3061 ttctcctcac ggtcccacgg aaaatgcggc tgcgcagcct gaattcaagc cggaatgccg
     3121 taaccgcact ggcattttcg atgttataaa atatttatca atttttaatt ttgcaaatat
     3181 ttaataacac gcagaacggc ggtgcggtca ggcagttttg accacaaccg gcggtcatgt
     3241 tcccgctccg tgcggggcgg gaatgcgtaa actgcacgcc cgaaactcat gcgatgagat
     3301 ggcgacatgg ccggtgcaag tcacgcagcg atcacgaaga tggtgaccga ctcgatcgag
     3361 gtcaagcaga aactgctggc cgaccgcgcg ctgctcgacg ccattgcgcg cgtgtgcgat
     3421 gtgtgcgtgg atgcattgaa gcgcggcaac aagatcatct tcgccggcaa cggcggcagc
     3481 gcggccgact cgcagcacct ggcggcggag ttcgtcgcgc gtttcgaatt cgaccggccg
     3541 gggctggcct cgatctcgct cacggtcgac acctcggcgc tcaccgccat cggcaacgac
     3601 tacggctacg agttcgtgtt cagccgccag ctggagtcca atggaaaatc cggtgacgtg
     3661 ttcttcggcc tgtccacctc gggcaactcg aagaacatca tcaaggcggt cgagcaggcg
     3721 aagaagatcg gcgtgaccac ggtggcgctg tgcggcgccg gcggcaagct gaaggacatg
     3781 tgcgaccacg cgctgtgcgc gccgtccacg cacacgccgc gcatccagga atgccacggg
     3841 ctgatcgggc acatcatctg cgcgctgtgc gaagagttga tgtaccccga gtacaagaag
     3901 cccaaggtgt aggagcgggc cttgcccgcg atgcgcgcca tgctcgcggg caaggcccgc
     3961 tcgtgcagcc gttgcgaaaa tatcgcactt atcggttaga ctcggtcact atcgcaattg
     4021 gcgattaatt atccagcccg cgagctgggc gacgtggtaa ccatggagac gattcctgca
     4081 atcctgaagc tcgatgccgg cggcctgccg gtgtcgtgga ttcgctggca gacggcggtc
     4141 acgctgtacg cgcgcgaccg cgtgcgctgg gaagccggcg acggccgctt caccatccac
     4201 ggcggcatct gcgcgcgcac cgggctgcag agcaggatcg agatcggcag catcatcgcg
     4261 gtggccgaca agtccaagcg tttcgagaag ggcgtgccgc tgctgaccaa tcgcacgctg
     4321 ttccagcgcg accgcaatct gtgcctgtat tgcggcgagc agttcccggt gtcgtacctc
     4381 acgcgcgacc acgtgatgcc cgcgtcgcgc ggcggccaga gcgtgtggga gaactgcgtg
     4441 accgcgtgcc gcgactgcaa ccagcgcaag gacgaccgca cgccggacga agccaacatg
     4501 aagttgctgg cggtgccgta cacgcccaac ctggccgagt acctgatcct gcagaaccgc
     4561 cgcatcctcg ccgaccagat ggatttcctg ctggcctacg cacgcaagca cgggcaccac
     4621 ggcggccgcc acgcagcctg aggcggcacg ctaggcggtc cgcgcgcgcc gtgcgcggca
     4681 gaactcaacc accgacccga tcacggcggc atcgcgcagg atgcgccggt gcccgagccg
     4741 ctcggtggca tggaagcgcg cgccgggcag gctgcgcgcc acctgcgcgc cctcggcaaa
     4801 cgggatcacc ttgtcgtcca cgtcatgcac caccagcgcg gcgacgttga gcatgcgtgc
     4861 cagccgcgaa atcgagcaca tctccaccgg catgtcgtag tggcgctcca gcgcggcgac
     4921 cagcgcggcc acgcatcgtg gcggcaggtc cagtgcctgc gccagccggc ggatgtcggc
     4981 tgccaactcg ccgcccggcg cgatcagcac caggcgcgcg gcagcatggc ggccgtcggc
     5041 cagcagctgg accgcggccg cagcgcccag cgaatgcgca atgacgccgt aaaccgggcc
     5101 gacgtgatcg gcgaccgcgc tcagcgcttc cgccatctgg tgcacatcgg tgcggcgcaa
     5161 g
//
LOCUS       DASZZC010000771         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_576897,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000771 DASZZC010000000
VERSION     DASZZC010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576897"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1021)
                     /locus_tag="VHE37_16615"
     CDS             complement(398..1021)
                     /locus_tag="VHE37_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT37210.1"
                     /translation="MSTVKSAASHSAAPEKTEETRGARARIFETARDLFYRQGLRAGG
                     VETIAKEAGATKMSLYRHFPSKDELTAECLKADREEFNEWWDSVMTPHAGNPRKQLEA
                     LFQEFQSKTCGDEDESRGCPLANAAVELADHAHPGWHVVFEHKSDVRERLQQLCREMK
                     ARNPKQLGDALMLLMEGAYISRLVFDMRGPVQSVSKAAKVLIEDQLA"
     gene            1173..2399
                     /locus_tag="VHE37_16620"
     CDS             1173..2399
                     /locus_tag="VHE37_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT37211.1"
                     /translation="MKKPNTLTAALYAAGLSLAVLTVAACSSSQAQPSAAPPAPSVSV
                     AQVVLRDVTQWDEFNGRVQAVESVEIRPRVSGYIDRVNFAEGKEVHKGDVLFVIDQRS
                     YRAELERAQADLARTRAQADLAHAEAARAEKLIELHAISREEHDERVAADAQAGAAVR
                     AAEAAASIARLNLEFTEVRSPIDGRAGQALVTAGNLVSAQPAATLLTTVVSLDPVYVY
                     FEGDEQTYLRYGELARRGERPSSRDARNPVFVGLGNEDGFPHEGVMDFVDNQVDPATG
                     TIRARAVLSNKDRVFSPGLFARVKLVGSGTAPALLIDDKAVLTDQDRKYVYVLGADNK
                     AERRDVKIGRLNDGMRVVLEGLSAGDKVIVHGVQKIFFPGMQVAPETIAMGEGPRDGE
                     PIRIAVSKSGKAEPRS"
     gene            2396..>4240
                     /locus_tag="VHE37_16625"
     CDS             2396..>4240
                     /locus_tag="VHE37_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT37212.1"
                     /translation="MNFSRFFVERPIFAGVISIIIFVGGLIALPILPISEYPEVVPPT
                     VQVRAVYPGANPKVIAETVATPLEEAINGVENMIYMKSVAGSDGVLVTTVTFKPGTDV
                     DLAQVQVQNRVSQAQARLPEDVRRLGVTTAKSSPNLTLVVHLVSDGRYDMVYLRNYAV
                     LHIKDELARLPGVGDTRIFGAGDYSMRIWLDPDKVAARGLTAGDVVNAIRDQNVQVSA
                     GQLGAPPQPTGTDFTLSINAQGRLVTEQEFGDIVVKAGADGQTTHLRDVARLELGASE
                     YALRSLLDNKQAVAIPVFQAPGSNALELSNRVRAKMAELATRFPEGVSWQVIYDPTVF
                     VRDSIKAVVHTLAEAVLLVVLVVILFLQTWRASIIPLLAVPVSIVGSFAVLLLLGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENVERNIERGLQPKAAAHQAMREVSGPIVAIALVL
                     TAVFVPMAFLSGVTGQFYRQFAVTIAISTVISALNSLTLSPALAAVLLRPHGAAPDVP
                     TRVIDRLFGWLFRPFNRFFSSASHAYSAGVAKVLRRGTLMFALYGGLVALTWAAFQAV
                     PGGFIPMQDKLYLIGIVKTPEGSSLDRTEALARKMSEIALGTEGVANAV"
BASE COUNT          686 a         1422 c         1385 g          747 t
ORIGIN      
        1 gcttccgccg cgcccctaaa tgcgcatcaa accatctgtc tcaccagcta acctcgcggc
       61 gccgattttt cttttcgttc cgcgggcttc cgccttcttc cgggttcttc cgggttgcat
      121 cacagcatcc gtcccgatgc atgaatgcga acactggaga cgccgcgact ggcaatttgt
      181 gtccctcctt ttcgagaaat tctccgtttc ccaaattggc agttcactta ctgatgatct
      241 tgcggatacc tgcgacgtcg gtttgatcgg atagagagaa cctgcgagta gaactccagc
      301 tgaaacttca gctgcactgc tggcagcagc gttcgggccc aggcttcgct tgttgtactc
      361 acgctgttct ggtgcgagga acggcgaggg ctggatgcta ggccagctga tcctcgatca
      421 gcaccttggc cgccttcgac acggattgca ccgggccgcg catgtcgaat accaagcgcg
      481 agatataggc gccttccatc agcagcatca gcgcatcgcc cagctgcttg gggttgcgcg
      541 ccttcatttc gcggcatagc tgctgcaggc gttcgcgcac gtcgctcttg tgctcgaaca
      601 ccacgtgcca gcctggatgc gcgtggtctg ccagttcgac cgccgcattg gccagcgggc
      661 agccgcgcga ctcgtcttcg tcgccgcagg tcttcgactg gaattcctgg aacagcgcct
      721 cgagctgctt gcgcggattg ccggcgtgcg gcgtcatcac gctgtcccac cactcgttga
      781 actcctcgcg gtcggccttc aggcattccg ccgtcagttc gtccttcgac gggaagtgac
      841 ggtacaggct catcttggtg gcgccggctt ccttggcgat cgtttccaca ccccccgcgc
      901 ggaggccctg gcgatagaac aagtcgcgcg cggtttcgaa gatgcgcgcg cgcgcaccgc
      961 gcgtctcctc ggttttttcc ggggcggctg aatgactggc agcggatttg acggtcgaca
     1021 tggcggaaac ctcttgacat gagagtgacc ggtcagtatc gtgcctcgcc attgataccg
     1081 aacggtatag tcacatcgag attctggtcg ggtgacggcg gcaagtcaag ccgcaccggc
     1141 ggtcaagcaa agcaagaggc aaggagtccg caatgaagaa gccaaatacc ctgaccgccg
     1201 cgctgtacgc cgccggcctt tccctggcgg tgctgaccgt cgccgcctgc agcagcagcc
     1261 aggcccagcc cagcgccgcg ccgccggcgc ccagcgtcag cgtcgcgcag gtggtgttgc
     1321 gcgacgtgac ccagtgggac gagttcaacg gccgcgtgca ggcggtggaa agcgtggaaa
     1381 tccgcccgcg cgtctccggc tacatcgacc gcgtgaactt cgccgaaggc aaggaagtgc
     1441 acaaaggcga cgtgctgttc gtcatcgacc agcgttccta tcgcgccgag ctcgagcgcg
     1501 cgcaggcgga cctggcccgc acccgcgccc aggccgacct ggcgcatgcc gaggccgccc
     1561 gcgccgaaaa gctgatcgag ctgcacgcca tttcgcgtga agagcatgac gagcgcgtcg
     1621 cggccgacgc ccaggccggc gccgcggtgc gtgccgccga agcggccgcc tccatcgccc
     1681 gcctgaacct tgaattcacc gaagtgcgct cgccgatcga cggccgcgcc gggcaggcac
     1741 tggtcactgc cggcaacctg gtgtcggcgc agccggcggc gacgctgctg accaccgtag
     1801 tgtcactcga tccggtgtac gtgtatttcg aaggcgacga gcaaacctac ctgcgctacg
     1861 gcgagttggc gcgccgcggc gagcgtccga gctcgcgcga cgcgcgtaac ccggtgttcg
     1921 tcggtctggg caacgaggac ggctttccgc acgaaggcgt gatggatttc gtcgacaacc
     1981 aggtcgaccc cgccaccggc accatccgcg cgcgcgcggt gctgtccaac aaggaccgcg
     2041 tgttttcacc cgggctgttc gcgcgcgtga agctggtggg cagcggcacg gcgccggcgc
     2101 tgctgatcga cgacaaggcc gtgctcaccg accaggaccg caagtacgtc tacgtgctcg
     2161 gtgcggacaa caaggcggag cgccgcgacg tcaagatcgg ccggctcaac gacggcatgc
     2221 gcgtggtgct cgaaggcctg agcgccggcg acaaggtcat cgtgcatggc gtgcagaaga
     2281 tcttcttccc cgggatgcag gtggcgcccg agaccatcgc catgggcgag ggcccgcgtg
     2341 acggcgagcc gatcaggatc gccgtcagca agagcggcaa ggcggagccg cgctcgtgaa
     2401 cttctcgcgc ttcttcgtcg agcgcccgat cttcgccggc gtgatctcga tcatcatctt
     2461 cgtcggcggc ctgatcgcgc tgccgatcct gccgatcagc gaataccccg aggtcgtgcc
     2521 gccgaccgtg caggtgcgcg cggtctatcc cggcgccaat cccaaggtca tcgccgaaac
     2581 cgtggccacg ccgctagagg aagcgatcaa cggcgtcgag aacatgatct acatgaagtc
     2641 ggtcgccggc agcgatggcg tgctggtgac caccgtcacc ttcaagcccg gcaccgacgt
     2701 cgacctggcg caggtgcagg tgcagaaccg cgtgagccag gcgcaggcgc gcctgccgga
     2761 agatgtgcgc cgcctgggcg tgaccacggc caagagctcg cccaatctga cgctggtggt
     2821 gcacctggtt tccgacggcc gctacgacat ggtgtacctg cgcaactacg cggtgctgca
     2881 catcaaggac gagctcgcgc gcctgcccgg cgtgggcgac acgcgcatct tcggcgccgg
     2941 cgattactcg atgcgcatct ggctcgatcc ggacaaggtg gccgcgcgcg gcctcactgc
     3001 cggcgacgtg gtcaacgcca tccgcgatca gaacgtgcag gtctcggccg gccagcttgg
     3061 cgcgccgccg cagccgaccg gcaccgactt cacgctgtcg atcaacgcgc aggggcggct
     3121 ggtcaccgag caggaattcg gcgacatcgt ggtcaaggcc ggcgccgacg gccagaccac
     3181 gcatctgcgc gacgtcgcca ggctggaact cggcgcgtcc gagtacgcgc tgcgttcgct
     3241 gctcgacaac aagcaggcgg tcgccatccc ggtgttccag gcgcccggct ccaacgcgct
     3301 ggagctgtcc aaccgcgtcc gcgccaagat ggcggaactg gccacacgct tccccgaagg
     3361 cgtgagctgg caggtgatct acgacccgac cgtgttcgtg cgcgattcga tcaaggcggt
     3421 ggtgcacacg ctggccgaag ccgtgctgct ggtggtgctg gtggtcatcc tgttcctgca
     3481 gacctggcgc gcctcgatca ttccgctgct ggcggtgccg gtgtccatcg tcggcagctt
     3541 tgccgtgctg ctgctgctcg gattctcgat caacacgctg acgctgttcg gcatggtgct
     3601 ggcgatcggc atcgtggtcg acgacgccat cgtcgtggtg gaaaacgtcg aacgcaacat
     3661 cgagcgcggc ctgcagccga aagcggcggc gcaccaggcc atgcgcgaag tcagcggacc
     3721 gatcgtcgcc atcgcactgg tgctgacggc ggtgttcgtg ccgatggcgt tcctgtccgg
     3781 cgtcaccggc cagttctacc ggcagttcgc ggtgaccatc gccatctcga cggtgatctc
     3841 ggcgctcaac tcgctgacct tgtcgccggc gctggccgcg gtgctgctca ggccgcatgg
     3901 cgccgcgccc gatgtgccga cgcgcgtgat cgaccgcttg ttcggctggc tgttccgtcc
     3961 gttcaaccgc ttcttcagca gcgcttcgca cgcctattcg gccggcgtgg ccaaggtcct
     4021 gcgccgcggc acgctgatgt tcgcgctgta cggtggactg gtggcgctga cctgggccgc
     4081 gttccaggcg gtgcccggcg gcttcattcc gatgcaggac aagctgtacc tgatcggcat
     4141 cgtcaagacg ccggaaggct cttcgctcga ccgcaccgag gcgctggcgc gcaagatgag
     4201 cgagatcgcg ctgggcaccg agggtgtggc caacgcggtc
//
LOCUS       DASZZC010000772         4515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_885232,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000772 DASZZC010000000
VERSION     DASZZC010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885232"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VHE37_16630"
     CDS             complement(<1..626)
                     /locus_tag="VHE37_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HVT37213.1"
                     /translation="MKPSYRHAHYSMDRWSHEAARISARRRPLLAGPWLLLALLLLAA
                     MSMSAHAERVQDLADVGGVRDNPLVGYGLVVGLDGTGDQTSQAPFTVQSLRAMLTQLG
                     VTVPANVNPQLKNVAAVAIHAQLPAFAKPGQKIDVTVSSLGNAASLRGGALLMTPLKG
                     ADGQVYAIAQGNVIVGGLGVSGSDGSKVTINVPSAGRIPGGANVERTVG"
     gene            complement(637..1344)
                     /gene="flgH"
                     /locus_tag="VHE37_16635"
     CDS             complement(637..1344)
                     /gene="flgH"
                     /locus_tag="VHE37_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HVT37214.1"
                     /translation="MILTRECLWDYLSLALLGLITLGVLAMLGGCASAGAREQPIEQA
                     LPPVAVAQPSDGAIYASGGGMSLFEDTRARNVGDILTIVLVESTAAKSSAETKTGKTT
                     SASISAPKIFGREVTIHGTSVLSGALDSDHSFDGSGDTSQSNTLSGNVTVTVVARQAN
                     GNLVVRGEKHLVLNQGNETVRVEGIVRPADIAPDNSIVSSRVADARISYDGKGTLAES
                     NTKGWLARFFSSPLMPF"
     gene            complement(1352..2137)
                     /gene="flgG"
                     /locus_tag="VHE37_16640"
     CDS             complement(1352..2137)
                     /gene="flgG"
                     /locus_tag="VHE37_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HVT37215.1"
                     /translation="MNQALWVAKTGLDAQQTRMTVVSNNLANVNTTGFKKGRASFEDL
                     LYQTAKQPGAQTSQTTQSPTGMMLGTGTHVVSTDKNFTQGSLQQTGNPLDVAVNGRGF
                     FQVSLPDGTAAYTRDGSFQVSSQGQLVTSSGYTVQPGITIPSAAQSVTIGSDGTVSVQ
                     LAGTAAPQQVGSLQLADFINPAGLQARGENLYLETAASGAPQTGTPGVSGLGTVAQGS
                     LESSNVNIVEEMVNMIETQRAYEINSKAVQTTDAMLQYVNNNL"
     gene            complement(2344..3087)
                     /locus_tag="VHE37_16645"
     CDS             complement(2344..3087)
                     /locus_tag="VHE37_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HVT37216.1"
                     /translation="MDKALYVAMTGAAQTMRAQAANNHNLANASTVGFRAELVTNSPT
                     AVPGDALGSRFDANLQDAGFDSNGGALMSTGRDLDVALRGNTWLAVQGSDGKEAYTRA
                     GDLTVTAQGQVINGAGHQVLGDGGLLSVPPNSSVTIGPDGTVSVVPLGQSAATLATVG
                     RLKLVDAAPGQLQRGEDGLMRAKAGAKLQPASGNVVQSGVLESSNVNLAEAMTTMISL
                     ARQYEMQVKMMHTVEDNATASASLVRMSQ"
     gene            complement(3114..4334)
                     /gene="flgE"
                     /locus_tag="VHE37_16650"
     CDS             complement(3114..4334)
                     /gene="flgE"
                     /locus_tag="VHE37_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HVT37217.1"
                     /translation="MPFRIALSGLNAASADLSTTANNIANANTTGFKGSRAEFADFFP
                     VSAYGLESNAIGGGTRLAKVAQQFSQGAVNFTNNSLDLAISGNGFFTLSDNGSTVYTR
                     NGAFGTDANGYVVNSSNQRLQINPPLANGAGFNTGTLTDLQLSTADHPPAASTKISAN
                     FNLRSDATPPANATFDPNDATSYNNTTSVTVYDSLGSAHTANLYFVKGATANTWNMYT
                     QIDGTAVGGAQTLAYSATGALTTPASGNITLPAYTPTTGAADMNLTLALGSTSQFGDT
                     FSVSELTQDGFATGRLTGLEISADGVVQARYTNGQATPLGQLSLANFDNPQGLQQLGN
                     ASWGETHSSGTALRGQPGTSNFGSIQSGALESSNVDLTEQLVNMITAQRNFQANAQMI
                     QTADQITQTIINIR"
BASE COUNT          633 a         1452 c         1603 g          827 t
ORIGIN      
        1 ccgaccgtgc gctcgacatt ggcgccgccg ggtatgcgcc cggcggacgg cacgttgatg
       61 gtgaccttgg agccgtcgct gccggatacg ccgagaccac cgacgatgac gttgccctgc
      121 gcgatggcgt agacctggcc gtcggcgccc ttgagcggcg tcatcagcag cgcgccgccg
      181 cgcagcgacg cggcattgcc cagcgacgat acggtgacgt cgatcttctg gcccggcttg
      241 gcgaaggccg gcagctgcgc gtggatggcc acggcggcca cgttcttcaa ttgcggattg
      301 acgttggccg gaacggtcac gcccagttgc gtgagcatgg cgcgcaggct ctgcacggtg
      361 aacggcgcct ggctggtctg gtcgccggtg ccgtcgaggc cgacgacgag tccatacccg
      421 accagcgggt tgtcgcgcac gccgcccacg tcggcgagat cctgcacgcg ctcggcgtgt
      481 gcgctcatcg acatggcggc gagcagcagc agcgccagca gcagccacgg gccggccagc
      541 agcgggcggc ggcgcgcgga aatgcgcgcg gcctcgtgcg accagcggtc catcgaataa
      601 tgtgcgtggc ggtagctcgg tttcatcccg gcactcctag aacggcatca gcggcgacga
      661 gaagaagcgc gccagccagc ccttggtgtt gctctcagcc agcgtgccct tgccgtcgta
      721 cgagatgcgc gcgtcggcca cgcggctgga aacgatgctg ttgtccggcg cgatgtcggc
      781 ggggcgcacg atgccttcca cgcgcaccgt ctcgttgccc tggttgagca ccaggtgctt
      841 ctcgccgcgc accaccaggt tgccgttggc ctggcgcgca accacggtga cggtgacgtt
      901 gccggacagc gtgttcgact gcgaggtgtc gccgctgccg tcaaacgagt gatcggaatc
      961 cagcgcgccc gacagcaccg aggtgccgtg gatggtgacc tcgcggccga agatcttcgg
     1021 cgcgctgatg ctggcgctgg tggtcttgcc ggtcttggtt tcggccgacg acttggccgc
     1081 ggtgctctcc accagcacga tggtgaggat gtcgccgacg ttgcgcgcgc gggtgtcctc
     1141 gaacagcgac atgccgccgc cggatgcgta gatggcgccg tcgctcggct gcgccaccgc
     1201 caccggcggc agcgcctgct cgatcggctg ctcgcgcgcg ccggccgacg cgcagccgcc
     1261 gagcatggcc agcacgccca gcgtgatcag gcccagcagg gccagcgaca ggtagtccca
     1321 caggcattcg cgcgtgagga tcatggcgcc ctcacaggtt gttgttgacg tactgcagca
     1381 tggcgtcggt ggtctgcacc gccttgctgt tgatctcgta ggcgcgctgc gtttcgatca
     1441 tgttcaccat ttcctcgacg atgttcacgt tcgacgattc cagcgagccc tgcgcaaccg
     1501 tgcccagccc gctcaccccc ggcgtgccgg tctgcggcgc gccgctggcg gcggtctcga
     1561 ggtacaggtt ttcgccgcgt gcctgcaggc cggcgggatt gatgaagtcg gccagctgca
     1621 gcgagcccac ctgctgcggc gccgcggtgc cggccagctg cacggagacg gtgccgtcgc
     1681 tgccgatggt cacgctctgc gccgccgacg gaatggtgat gcccggctgc acggtgtagc
     1741 cggacgaggt gaccagctgg ccctggctgc tcacctggaa gctgccgtcg cgcgtgtacg
     1801 cggcggtgcc gtcgggcagc gacacctgga agaagccgcg gccgttgacc gccacgtcga
     1861 gcgggttgcc ggtctgctgc agcgagccct gggtgaaatt cttgtcggtg gacaccacat
     1921 gcgtgccggt gccgagcatc atgccggtgg gcgactgcgt ggtctggctg gtctgcgcgc
     1981 ccggctgctt ggccgtctgg tacagcaggt cctcgaacga cgcccggccc ttcttgaagc
     2041 cggtggtgtt cacgttggcc agattgttgg agaccaccgt catgcgcgtc tgctgcgcat
     2101 cgagcccggt cttcgccacc cacagtgcct gattcatgac gccactcctg cggcggaaat
     2161 ttgccgccgc tggcgtctct acaacttccg ggccagacca aaacccttcc accgcactgc
     2221 ccagatcgtt gtttttattt gtattatgaa tttactttca gataataatt aataacatcg
     2281 caaaccgcgg tgcggtggac tgtttttcag cgatttactg cgccgcgcga gacgcggcgg
     2341 aggctactgg ctcatgcgca ccagcgaggc cgacgcggtg gcgttgtcct ccaccgtgtg
     2401 catcatcttc acctgcatct cgtactggcg cgccagcgag atcatggtgg tcatcgcctc
     2461 agccaggttg acgttggagg attccagcac gccgctctgc accacgttgc cggatgcggg
     2521 ctgcagcttg gcgccggcct tggcgcgcat cagcccatcc tcgccgcgct gcagctggcc
     2581 gggcgccgcg tcgaccagct tgagccggcc gacggtggcg agcgtcgccg cgctctgtcc
     2641 cagcggcacc accgacacgg tgccatcggg accgatggtc acggaagaat tcggcggcac
     2701 cgacagcagg ccgccgtcgc ccagcacctg gtgcccggcg ccgttgatca cctgcccctg
     2761 cgcggtcacg gtgagatcgc cggcgcgcgt gtacgcctcc ttgccgtcgg atccctgcac
     2821 cgccagccag gtgttgccgc gcagcgccac gtcgaggtcg cgcccggtgg acatcagcgc
     2881 gccgccgttg gaatcgaaac cggcgtcctg caggttggcg tcgaagcgcg agcccagcgc
     2941 atcgcccggc accgcggtcg gcgagttggt caccagctcg gcgcggaagc cgacggtgct
     3001 ggcgttggcc aggttgtggt tgttcgccgc ctgcgcgcgc atggtctgcg cggcgccggt
     3061 catggcgaca tacagtgcct tgtccatggg tcagtccgcc ggcgctgcgc tcatcaacgg
     3121 atgttgatga tggtctgcgt gatctggtcc gccgtctgga tcatctgcgc gttcgcctgg
     3181 aagttgcgct gcgcggtgat catgttcacc agctgctcgg tgagatcgac gttggacgat
     3241 tccagcgcac ccgactggat cgagccgaag ttgctggtgc ccggctggcc gcgcagggcg
     3301 gtgccggagc tgtgcgtctc gccccagctg gcattgccca gctgctgcag gccctgcggg
     3361 ttgtcgaagt tggccagcga cagctggccc agcggcgtgg cctggccgtt ggtgtagcgc
     3421 gcctgcacca cgccgtcggc ggaaatctcc aggccggtga ggcggccggt ggcaaagccg
     3481 tcctgggtca gttcgctgac cgagaaggta tcgccgaact ggctggtgct gcccagcgcc
     3541 agcgtcaggt tcatgtcggc cgcgccggtg gtcggcgtgt acgcgggcag cgtgatgttg
     3601 cccgaggccg gcgtggtcag cgcgccggtg gcggaatagg ccagcgtctg cgcgccaccg
     3661 acggcggtgc cgtcgatctg cgtgtacatg ttccaggtgt tggcggtggc gcccttcaca
     3721 aagtagagat tggccgtgtg cgcgctgccc agcgagtcat acaccgtcac cgaggtggtg
     3781 ttgttgtagc tggtggcgtc gttcgggtcg aacgtggcgt tggccggcgg cgtggcgtcg
     3841 ctgcgcaggt tgaagttggc gctgatcttg gtcgaggccg ccggcggatg gtcggcggtg
     3901 gacagctgca ggtcggtgag cgtgccggtg ttgaagccgg cgccgttggc cagcggcggg
     3961 ttgatctgca ggcgctggtt gctgctgttg accacgtagc cgttggcatc ggtgccgaag
     4021 gcgccgttgc gggtgtacac ggtggaaccg ttgtccgaca gcgtgaagaa gccgttgccg
     4081 ctgatcgcga ggtccagcga gttgttggtg aagttcaccg cgccctggct gaactgctgc
     4141 gccaccttgg ccaggcgcgt gccgccgccg atggcgttgc tctccaggcc ataggccgac
     4201 accgggaaga agtcggcgaa ttccgcgcgc gatcccttga agccggtggt gttggcgttg
     4261 gcgatgttgt tggcggtggt cgacaggtcg gccgaggcgg cgttgaggcc ggacagtgca
     4321 atgcggaagg gcatggtgga ctcctttgat tagaaaatct ggcgcacgtc ggagaacgcg
     4381 acgccggaca tgccggtgag atcgagttcg agtccgtcgc tgccgacagc cacgctgtcg
     4441 acatggccga cggcgtaagt ggtgagggcc tgttgcgcgc cggtcgttcc ggccagcgcg
     4501 ctgatctggt agctg
//
LOCUS       DASZZC010000773         8976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_577138,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000773 DASZZC010000000
VERSION     DASZZC010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577138"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..342)
                     /locus_tag="VHE37_16655"
     tRNA            complement(253..342)
                     /locus_tag="VHE37_16655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Ser,seq:cga)
     gene            397..1905
                     /gene="mltF"
                     /locus_tag="VHE37_16660"
     CDS             397..1905
                     /gene="mltF"
                     /locus_tag="VHE37_16660"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HVT37218.1"
                     /translation="MNAAANPKPADPEVASRRPAFWFFLIFGGIAYAVLSTCSPRTGT
                     LSMVKSLGVMKVATVNSPTSYYVGPNGPTGFDYDLAKGFAERLGVQLQIEEASGPAEA
                     LAWVRDGRVNFAAAGLNVTAAREKQVHFSHPLLSVVPQLVYALGLPKPKDLGELDGRL
                     RVPRGSVAAERLRELKKTTFPSLNWEESDDQEVETLLYQVANEQLNYTIANSDIIAIN
                     QHYYPKLRVAFDLSDAQDIAWAFPQDDDSSLYDAADDYLRDTGGAQLARLRDRYFGHV
                     EQVDYYGSVTLAADVRSRLPRYRALFEKAAAKFNLDWRLLAAMGYQESHWDPNAISPT
                     GVRGIMMLTNDTATLLNIADRVDPAQSIAGGARYFHDIVEQIPDEIADPNERTWMALA
                     AYNMGIGHLLDARELTRSLGGDPNHWLDVRNTLALLTQPKWYSKLKYGYARGFEGVTY
                     VGNVRTYYDMLMWIESGGAGPRHVEVEPPLQPPTATPQAKKRDLLDINTPVL"
     gene            complement(2049..2378)
                     /locus_tag="VHE37_16665"
     CDS             complement(2049..2378)
                     /locus_tag="VHE37_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HVT37219.1"
                     /translation="MSEPESKSYYRHHVFFCCNQRPPGERTCCNDKGATRVRDHAKKR
                     IRQLGLAAPGKVRINQAGCMERCEQGPCVVVYPEGVWYTYVDEEDMDEIIDQHIVGGR
                     VVERLRI"
     gene            2400..3644
                     /locus_tag="VHE37_16670"
     CDS             2400..3644
                     /locus_tag="VHE37_16670"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVT37220.1"
                     /translation="MSALPSTGEKFATSFPLEQADQCVKCGLCLPHCPTYLQTQHEGD
                     SPRGRIALMQGLAMNLVPLSEPLERHLDGCLSCRACERVCPAKVPYGHLIDQGRQMLA
                     QRKPERTRTTRAIGFALTSRTLRNAGGALLWLYQRSGLQWLVRTLHLLGRGPLARMES
                     LLPRVSFPRFPSAAIAAAKPAVQLFTGCAGELFDGHTLRDASQLLSRLGWSVEIPRAQ
                     ACCGAIHQHGGETHEAARFVARNLAAFDGDAPVLCSASGCGATLLDYPDIVDGPAAKK
                     FRARVRDIDAFLLEHWPASAQLKPLAQRIAVHTPCTLKNVMKSDAAVTQLLKKIPQAD
                     VFELDAAQHCCGAAGSYFITQPEMADRLLQRKLDALAAQQPDLLVSTNIGCTLHIAGG
                     LRRRGLTVPVVHPVTLLAHQLA"
     gene            complement(3592..3948)
                     /gene="lptE"
                     /locus_tag="VHE37_16675"
     CDS             complement(3592..3948)
                     /gene="lptE"
                     /locus_tag="VHE37_16675"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HVT37221.1"
                     /translation="MIQLSDLFETRSVLSIGADGKALEYQLTTSVRYLVSSGGKALLA
                     PDVLTASRDYSYNATQVLAKEAEESRLREFIQNELAELLLLRLEAGFAHLPAAADGTL
                     TPADVPAVSPGAPPGQ"
     gene            complement(3961..4164)
                     /locus_tag="VHE37_16680"
     CDS             complement(3961..4164)
                     /locus_tag="VHE37_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37222.1"
                     /translation="MRNLSATLLVLLLAGCGFHLAGNRPLPPQLKSVYIDVVAPYRVS
                     EPPLESSLRGLLLRRGGRVYSKA"
     gene            complement(4164..6743)
                     /gene="leuS"
                     /locus_tag="VHE37_16685"
     CDS             complement(4164..6743)
                     /gene="leuS"
                     /locus_tag="VHE37_16685"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HVT37223.1"
                     /translation="MQEQYTPAEIERAAQAYWQQHKSFEVTEDPSRQKYYCLSMFPYP
                     SGRLHMGHVRNYTIGDVISRYQRMLGKNVLQPIGFDAFGLPAENAAIANRTQPAKWTY
                     ANMEYMRGQLRALGFAYDWSREVQTCRPEYYRWEQLLFTRLFRKGLVYRRNSVVNWDP
                     VDQTVLANEQVIDGRGWRSGAVVERREIPQWFIKITAYAQELLDELDRMPGWPEPVKI
                     MQRNWIGRSEGLELQFPVEGGEPLTVYTTRPDTLMGVTYLAVAAEHPLALQAAEKNPA
                     LAEFLKSCAQANVAESAMETMEKRGMALDISAVHPISGERVPVWAANFVLMGYGTGAV
                     MAVPGHDERDHEFARKYGLPIKQVIKPADGSTIDVAQAAYTEHGVCINSGEYDGLDYQ
                     HAFDAIAARFEKRSTGKRRVNFRLRDWGVSRQRYWGCPIPIIYCAQCDAVPVPEDQLP
                     VVLPEDVEFSGVKSPIKADPAWAKCTCPQCGGPATRETDTFDTFVESSWYYARYACPD
                     NDKQMLDARADYWLPADQYIGGIEHAILHLLYARFWYKLMRDQGMVKSDEPFTNLLTQ
                     GMVVKETYFREEDGKIHWINPADVEVTLDDRGHPLGAKLKSDGLPVQIGGVEKMSKSK
                     NNGLDPQTLIDRYGADVLRLFAMFASPPELSLEWRDDGVEGSARFLRRLWRAVHAHVS
                     AGAAPALDASALSAAQKNLRRQLHETIAKVSDDYGRRKTFNTAIAAVMELMNAANKLE
                     DGSAQGRALMQELWVAVTLMLNPIVPHVCHALWQALTGRDDLLAQPFPRADEKALIKD
                     SIALVVQVNGKLRGNVEVPASAGKDEIIAAALADAQVQKFIAGAPIKKQIMVPGKLVN
                     FVV"
     gene            6945..8741
                     /locus_tag="VHE37_16690"
     CDS             6945..8741
                     /locus_tag="VHE37_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37224.1"
                     /translation="MPESLRRHGALLLPALIALALYWPCVHFEFLWDDGDLLLRSASL
                     RQWSSLWSSINHAFLTYGGYYRPLPLLTLGLEQIAAPGPWLPHLDAVLLHALNCLLLG
                     LLARTVALQLGEEARAARIGLLAGAIYACHPVLIETVAWISSRFDLLLISFSLCLLLA
                     DRLLLGAARAITVSICFMAAALSKEMAVALPLLLPLWHLAFEPRAGWHETLAAFRRRG
                     HVQVYLGVLASGLLYLALRYQGLGRGMADTEWPVEKFSLLHLVLVLKTIGTDIMLMLF
                     PFTTLSPLHSYAGASAVDFYTWAGAAFALLCSGLALAVARGLRGPAILVLCCAATLLP
                     VSNIIPVPLRDCYTSERFLGLPLVFLALGLSIGLLRALSSMQDLRVLQRALPALLAVF
                     WFGLCVLNISVTLPLWRNSLSLWTWSYARAPDSPTAQINLTASYFALGDYGRARVFME
                     QVFQRQHDAGIAPDPDQYFQYLTTLVYTDAPSAAVKPLYAALEQLPPSRNLQVQLRRH
                     DQLSWLYLLRGHAPQAVRHFNAVLSIDPANPAAHFGLGVMQLAAGQQADGQNSIDYAM
                     QHGHPSIIAKWRAQLPQLITAARASSAHAEDL"
     gene            complement(8748..>8976)
                     /locus_tag="VHE37_16695"
     CDS             complement(8748..>8976)
                     /locus_tag="VHE37_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37225.1"
                     /translation="IASQETIADSTPAAELEPSAVDDLLTSRGRIPAGWYVSDDDNLE
                     LDYRLPRAAALQPALALGANLAELRRYAATR"
BASE COUNT         1412 a         3114 c         2863 g         1587 t
ORIGIN      
        1 gcttccgccg cgcccctaaa tgcgcatcaa accatctgtc tcaccagcta acctcgcggc
       61 gccgattttt cttttcgttc cgcgggcttc cgccttcttc cgggttcttc cgggttgcat
      121 cacagcatcc gtcccgatgc acacaaattg ccagtcgcgg cgtctccagt gttcgcattc
      181 aaagaaaaaa gcccgctgtt agcgggcttt tcccctcctg cggaccggtc atctctgacc
      241 cgtaggactt gctggcggag agggtgggat tcgaacccac ggtacgcttt cacgtacgcc
      301 tgatttcgag tcaggtacat tcaaccactc tgccacctct ccaagtaaac tggcttcgcg
      361 cggcggcatg gggcgggagc cgggcgcgaa ttctaaatga atgcggcagc gaatccaaaa
      421 ccagcagatc cagaggttgc cagccgccgt ccggcgttct ggttcttcct gatcttcggc
      481 ggcatcgcct acgcggtgct gtcgacctgc tcgccgcgca ccggcacgct gagcatggtg
      541 aagtcgctgg gcgtgatgaa ggtggccacg gtcaacagcc ccaccagcta ctacgtgggg
      601 cccaatggcc cgaccggctt cgactacgac ctggccaagg gattcgccga gcgcctgggc
      661 gtgcaattgc agatcgagga agccagcggc cctgccgaag cgctggcctg ggtgcgcgac
      721 ggccgggtga acttcgccgc cgccgggctc aacgtgaccg ccgcgcgcga gaagcaggtg
      781 cacttctcgc acccgctgct gtcggtggtg ccgcagctgg tctacgcgct gggcctgccc
      841 aagcccaagg acctgggcga gctcgacggc cgcctgcgcg tgccgcgcgg cagcgtggcg
      901 gcggagcggc tgcgcgaact gaagaagacc accttcccct cgctcaactg ggaggaatcc
      961 gacgaccagg aagtggagac gctgctctac caggtggcca acgagcagct caactacacc
     1021 atcgccaatt ccgacatcat cgccatcaac cagcattact accccaagct gcgcgtggcc
     1081 ttcgacctgt cggacgcgca ggacatcgcc tgggcctttc cgcaggacga cgacagctcg
     1141 ctgtacgacg ccgccgacga ttacctgcgc gacaccggcg gcgcgcagct ggcgcgactg
     1201 cgtgaccgct acttcggcca cgtggagcag gtggattact acggctcggt cacgctggcg
     1261 gccgacgtgc gctcgcgcct gccgcgttac cgcgcgctgt tcgagaaggc ggcggcgaag
     1321 ttcaacctcg actggcggct gctggcggcg atgggctacc aggagtcgca ctgggacccc
     1381 aacgccatca gccccaccgg cgtgcgcggc atcatgatgc tgaccaacga caccgccacg
     1441 ctgctcaaca tcgccgaccg cgtcgacccg gcgcagagca tcgccggcgg cgcgcgctac
     1501 ttccacgaca tcgtcgagca gattcccgac gagatcgccg atcccaacga gcgcacctgg
     1561 atggctctgg ccgcgtacaa catgggcatc ggccacctgc tcgatgcgcg cgagctgacg
     1621 cgctcgctcg gcggcgatcc caatcactgg ctcgacgtgc gcaacacgct ggcgctgctc
     1681 acccagccca agtggtacag caagctcaag tacggttacg cgcgcggctt cgaaggcgtg
     1741 acctacgtcg gcaacgtgcg cacctactac gacatgctga tgtggatcga gagcggcggc
     1801 gccggaccgc gccacgtgga ggtcgagccg ccgctgcagc cgccgacggc cacgccgcaa
     1861 gcgaagaagc gcgacctgct cgacatcaac acgccggtgc tgtgagctgg ctgcaccctg
     1921 gccgcacaag gcaaaaaaac ctgccaccgc acccgccccg gcactgttat taattattat
     1981 atgattatta aataaataaa taattcacaa catcgaaaat gccggtgcgg tgaggccaaa
     2041 aaagcgcttc agatgcgcag gcgctccacc acgcggccgc cgacgatgtg ctggtcgatg
     2101 atctcgtcca tgtcttcctc gtcgacgtag gtgtaccaga cgccttccgg gtagaccacc
     2161 acgcacggcc cctgctcgca gcgctccatg cagcccgcct ggttgatgcg caccttgccc
     2221 ggcgcggcca ggcccagctg cctgatgcgc ttcttggcgt ggtcgcgcac gcgcgtggca
     2281 cccttgtcgt tgcaacaggt gcgctcgccg ggcggccgct ggttgcagca gaagaacacg
     2341 tggtgccggt agtaggactt ggactcgggc tcgctcatgg cgccgctatt atagaagcca
     2401 tgtccgcgct gccctccacc ggcgaaaaat tcgccacctc gtttccgctg gaacaggccg
     2461 accagtgcgt gaagtgcggc ctgtgcctgc cccactgccc cacctacctg cagacccagc
     2521 acgagggcga ctcgccgcgc ggccgcatcg cgctgatgca gggcctggcg atgaacctgg
     2581 tgccgctgtc cgaaccgctg gagcggcatc tggacggttg cctgtcctgc cgcgcctgcg
     2641 agcgcgtgtg cccggcgaag gtgccgtacg gccacctgat cgaccagggc cggcagatgc
     2701 tggcccagcg caagcccgag cgcacgcgca ccacgcgcgc gatcggcttc gccctcacct
     2761 cgcgcacgct gcgcaacgcg ggcggcgcgc tgctgtggct gtaccagcgc agcggcctgc
     2821 aatggctggt gcgcacgctg cacctgcttg ggcgcgggcc gctggcgcgc atggaatcgc
     2881 tgctgccgcg ggtttccttt ccacgatttc catcggcggc catcgctgcc gccaagcctg
     2941 ccgtgcagct gttcaccggc tgcgcgggcg agctgttcga cgggcacacg ctgcgcgacg
     3001 cctcgcagct gctgtcgcgc ctgggctggt cggtggagat cccgcgcgcg caggcctgct
     3061 gcggcgccat ccaccagcac ggcggcgaaa cgcacgaagc tgcgcgcttc gtcgcgcgca
     3121 acctcgcggc cttcgacggc gatgcgcccg tgctgtgctc cgcctccggc tgcggtgcga
     3181 cgctgctcga ctaccccgac atcgtcgacg gcccggcggc aaaaaaattc cgcgcgcgcg
     3241 tgcgcgacat cgatgccttc ctgctcgaac actggcctgc cagcgcgcag ctcaaacccc
     3301 tggcacagcg catcgcggtg catacgccgt gcactttgaa aaacgtgatg aaaagcgacg
     3361 cggcggtcac gcagctgctg aagaagattc cgcaggcgga cgtgttcgag ctcgatgccg
     3421 cgcagcattg ctgtggcgcc gccggcagct atttcatcac ccagccggag atggcggacc
     3481 ggctgctgca gcgcaagctc gacgcgctgg ccgcccagca acccgacctg ctggtgagca
     3541 ccaacattgg ctgcacgctg cacatcgccg gcgggctgcg gcgccgggga ctcactgtcc
     3601 cggtggtgca cccggtgaca ctgctggcac atcagctggc gtgagcgtgc cgtcggccgc
     3661 cgccggcaga tgggcgaagc ccgcttccag gcgcagcagc agcagttccg ccagctcgtt
     3721 ctggatgaat tcgcgcaggc gcgactcctc ggcttccttg gccagcacct gcgtggcgtt
     3781 gtagctgtag tcgcgcgagg cggtgagcac gtcgggcgcc aacagcgcct tgccgccgct
     3841 ggacaccagg tagcgcacgc tggtggtcag ctggtattcc agcgccttgc cgtcggcgcc
     3901 gatcgacagc accgaacgcg tctcgaacag gtccgacagc tggatcaccg tgcccgcttc
     3961 ctacgccttg ctgtacacgc ggccgccgcg gcgcagcagc agtccgcgca acgaactctc
     4021 cagcggcggc tccgacacgc ggtatggcgc caccacgtcg atgtacaccg acttcagctg
     4081 cggcggcagc gggcggttgc cggccaggtg gaagccgcaa ccggccagca gcagcaccag
     4141 cagcgtggcg gagaggttgc gcatcagacg acgaagttca ccagcttgcc cggcaccatg
     4201 atctgcttct tgatcggcgc gccggcaatg aacttctgca cctgcgcatc ggccagcgcg
     4261 gcggcgatga tctcgtcctt gcccgcggat gccggcactt cgacgttgcc gcgcagcttg
     4321 ccgttgacct gcaccaccag cgcgatgctg tccttgatca gcgccttctc atcggcgcgc
     4381 ggaaagggct gggccaggag gtcgtcgcgc ccggtcaacg cctgccacag cgcgtggcac
     4441 acgtgcggca cgatgggatt gagcatcagc gtcaccgcca cccacagctc ctgcatcagc
     4501 gcgcggccct gcgcgctgcc gtcctcgagc ttgttggccg cgttcatcag ttccatcacc
     4561 gccgcgatcg cggtgttgaa ggtcttgcgc cggccgtagt cgtcgctcac cttggcgatg
     4621 gtttcgtgca gctggcggcg caggttcttc tgcgcggcgc tcagcgcgga cgcgtcgagc
     4681 gccggcgcgg cgccggcgct cacatgcgca tgcaccgcgc gccacaggcg gcgcaggaag
     4741 cgggccgaac cttccacgcc gtcatcgcgc cattccagcg acagttccgg cggcgaggcg
     4801 aacatggcga acaggcgcag cacatcggcg ccgtagcggt cgatcagggt ctgcggatcg
     4861 aggccgttgt tcttgctctt ggacatcttc tcgacgccac cgatctgcac cggcaggccg
     4921 tcggacttca gtttcgcgcc gagcggatgg ccgcgatcgt cgagcgtcac ttcaacatcg
     4981 gccgggttga tccagtggat cttgccgtcc tcttcgcgga aatacgtctc cttcaccacc
     5041 atgccctgcg tcagcaggtt ggtgaacggc tcgtccgatt tcaccatgcc ctggtcgcgc
     5101 atcagcttgt accagaagcg cgcgtacagc aggtgcagga tggcgtgctc gatgccgccg
     5161 atgtactggt cggccggcag ccagtaatcg gcgcgggcgt cgagcatctg cttgtcgttg
     5221 tccgggcagg cgtagcgcgc gtagtaccag ctcgattcga cgaaagtatc gaaggtgtcg
     5281 gtctcgcgcg tggccgggcc gccgcattgc gggcaggtgc atttcgccca ggcgggatcg
     5341 gccttgatcg gcgatttcac gccggagaat tccacgtctt ccggcagcac caccggcagc
     5401 tggtcttccg gcaccggcac cgcgtcgcac tgggcgcagt agatgatcgg gatcgggcaa
     5461 ccccagtagc gctggcgcga tacgccccag tcgcgcaggc ggaaattcac ccggcgcttg
     5521 ccggtgctac gtttttcgaa gcgcgcggca atggcgtcga acgcgtgctg atagtcgagg
     5581 ccgtcgtatt cgccggagtt gatgcagacg ccatgctcgg tgtacgcggc ctgcgccacg
     5641 tctatcgtgc tgccatcggc cggcttgatg acctgcttga tcggcaggcc gtacttgcgc
     5701 gcgaattcat ggtcgcgctc gtcgtgtccc ggcaccgcca tcaccgcgcc ggtgccgtag
     5761 cccatcagca cgaagttggc ggcccacacc ggcacgcgct cgccactgat cggatggacg
     5821 gcggaaatgt ccagcgccat gccgcgcttt tccatcgtct ccatggcgct ctcggccacg
     5881 ttggcctgcg cgcaggattt caggaactcg gccagcgccg gattcttctc cgccgcctgc
     5941 aacgccagcg gatgctcggc tgcgacggcc aggtacgtca cgcccatcag cgtgtccggg
     6001 cgcgtggtgt acaccgtcag cggctcgccg ccttccaccg ggaactgcag ctccaggcct
     6061 tcggagcggc cgatccagtt gcgctgcatg atcttcaccg gctccggcca gccgggcatg
     6121 cggtcgagtt cgtcgagcag ttcctgcgcg tacgcggtga tcttgatgaa ccactgcggg
     6181 atctcgcgcc gctccaccac cgcgccgctg cgccagccgc ggccgtcgat cacctgctcg
     6241 ttggccagca cggtctggtc gaccgggtcc cagttcacca ccgagttgcg gcgatagacc
     6301 aggcctttcc tgaacaggcg cgtgaacagc agctgctccc agcggtagta ctccgggcgg
     6361 caggtctgca cttcgcgcga ccagtcgtag gcgaagccca gcgcgcgcaa ttgcccgcgc
     6421 atgtactcca tgttggcgta ggtccatttc gccggctgcg tgcggttggc gatggccgcg
     6481 ttttccgccg gcaggccgaa ggcatcgaag ccgatcggct gcagcacgtt cttgccgagc
     6541 atgcgctggt agcggctgat cacatcgccg atggtgtagt tgcgcacgtg gcccatgtgc
     6601 aggcggcccg acgggtacgg gaacatcgac aggcagtagt acttctgtcg cgacgggtct
     6661 tccgtcacct cgaacgactt gtgctgctgc cagtacgcct gggcggcgcg ttcgatttca
     6721 gcgggcgtgt actgctcttg catcggcatt gcgcggcggc aaaaaaggtc gcgtagtgta
     6781 ctcgacgtgt ccaccgcagc cgcctgcagc ttgttacaaa ttattaatca tattaaaaat
     6841 taaacattat tttataacat acgaaaccag ggtgcggtgt gaccttttgc gcccctgcta
     6901 gcatgtcgcg cggcaccgcc gccaccaacc atcgatcgcg tcccatgccc gaatcactcc
     6961 gccgtcacgg cgcgctgctg ctgcccgcat tgatcgcgct ggcgctgtac tggccgtgcg
     7021 tgcacttcga attcctgtgg gacgacggcg acctgctgct gcgctcggcc agcctgcgcc
     7081 agtggtcgtc gctgtggagt tccatcaacc acgccttcct cacctacggc ggctactacc
     7141 gtccgctgcc gctgctgacg ctgggcctcg agcagatcgc ggcgccaggt ccatggctgc
     7201 cgcacctcga cgccgtgctg ctgcatgcgc tcaactgcct gttgctgggc ctgctggcgc
     7261 ggacggtggc gctgcagctc ggcgaagaag ctcgcgcggc gcgtatcggc ctgctcgccg
     7321 gcgcgatcta cgcgtgccac ccggtgctga tcgaaaccgt ggcgtggatc agctcgcgct
     7381 tcgacctgct gctcatcagc ttcagcctgt gcctgctgct ggctgaccgg ctgctgctcg
     7441 gcgcggcgcg ggcgatcacg gtatcgatct gcttcatggc ggccgcactg agcaaggaga
     7501 tggcggtggc gctgccgctg ctgctgccgc tgtggcacct ggcattcgag ccgcgcgccg
     7561 gctggcacga aacccttgcg gcattccgcc ggcgcggcca tgtgcaggtt tacctcggcg
     7621 tcctcgcaag cggcttgtta tacctggcgc tgcgctacca ggggctgggc cgcggcatgg
     7681 ccgacaccga atggccggtg gaaaaattct ccctgctgca cctggtgctg gtgctcaaga
     7741 ccatcggcac cgacatcatg ctgatgctgt ttcccttcac cacgctgagc ccgctgcaca
     7801 gttacgccgg cgccagcgcg gtggatttct acacctgggc cggcgccgct tttgccctgc
     7861 tctgttcagg actggcgcta gcagtcgcgc gcggcctgcg cggccccgcg attctggtgc
     7921 tgtgctgtgc cgcgacgctg ctgccggtgt ccaacatcat cccggtgccg ctgcgggact
     7981 gctacacctc cgagcgcttc ctcggcctgc cactggtctt cctcgcgctg ggcctgtcga
     8041 tcggcctgct gcgcgcgctg tcgtcgatgc aggacctgcg cgtgctccag cgcgcgctgc
     8101 cggcgctgct cgccgtcttc tggttcggcc tgtgcgtgct caacatcagc gtgacgctgc
     8161 cgctgtggcg caacagcctg tcgctgtgga cctggagcta cgcgcgcgcg ccggattcgc
     8221 cgaccgcgca gatcaacctg accgcttcgt atttcgccct cggcgactac ggccgcgcgc
     8281 gcgtgttcat ggaacaggtg ttccagcggc agcacgacgc aggcatcgcc cccgatcccg
     8341 atcagtactt ccagtacctg accacgctgg tctataccga tgcgccctcg gcagcggtga
     8401 agccgctgta tgccgcgctc gagcagttgc cgcccagccg caacctgcag gtgcagctgc
     8461 gccggcatga ccagctgagc tggctatacc tgctgcgcgg ccatgcgccg caggcagtgc
     8521 gtcatttcaa cgccgtgcta agcatcgatc cggccaaccc ggcggcgcac ttcgggctcg
     8581 gggtcatgca gctggccgcg ggccagcagg ccgatggcca gaattccatc gactacgcca
     8641 tgcagcacgg ccatccctcg atcatcgcga aatggcgggc gcaattgccg caactgatca
     8701 ccgcggcccg ggccagcagc gcacacgccg aagatctttg atcgaggtca gcgcgtggcc
     8761 gcgtagcggc gcagttcggc caggttcgcg cccagggcca gcgcgggttg cagcgcggcg
     8821 gcgcgcggca ggcgatagtc gagctcgagg ttgtcgtcgt cggacacata ccaacccgcc
     8881 gggatcctgc cgcggctggt cagcaagtca tcgaccgcgg agggctccag ttctgccgcc
     8941 ggcgtactgt ccgctatcgt ttcctgcgag gcgatg
//
LOCUS       DASZZC010000774         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_885877,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000774 DASZZC010000000
VERSION     DASZZC010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885877"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1238)
                     /locus_tag="VHE37_16700"
     CDS             complement(15..1238)
                     /locus_tag="VHE37_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HVT37226.1"
                     /translation="MIPFSLKVLIFKIYQFILKWATKVLTFRTPELYSGPGSSLRLCE
                     HIADTGVKKLLIVTDAMLVKIGLLKPLQEKLTQLGVNYVVYDGVQPNPTIEQIETGLA
                     MLKKEGCTAILAIGGGSSIDAAKVIAARATNPFMKIVHMAGLMRVVFKLLPLYVVPTT
                     AGTGSEVTIAAVVSDPSTTRKFAIMSPRLVPVGAALDGALMTGLPPPITSATGMDALT
                     HAVEAYISRNHTDMTDAEALDATRLIMANLQTAVKDGSNVEARQNMALASYKAGVAFT
                     TAGVGYVHAIAHNFGAYYHLPHGLANAIILPRVLDFSKPNCIERLAKLAEVSGLKKGG
                     ESDAQLADAFIAKVRALNAEFGIPTQVEKLKQSDIPAITDKALAEAHWTYAVPRYMDK
                     PECQAFIAQMLPQAA"
     gene            complement(1310..2698)
                     /locus_tag="VHE37_16705"
     CDS             complement(1310..2698)
                     /locus_tag="VHE37_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37227.1"
                     /translation="MRIAMLLLLAGMAGAAQAAVTDKGCAWIVEPTADRENILFPEIT
                     TRYLAAPLPAVPGGYVEIAGQFPHARYMSLQTYNLNLQTISDLRDVQIQPDAGSINPF
                     IEGADRTAQARNYTVRVVDQAVPASGGDANTLYYIGDSGTRVGFSVVYRIYLPDAGTQ
                     PFGAVPAPTLTEVLPGGTRVPIPTCPDVVPDTSFLTEELAASGLSDYPLPPVGLFAQP
                     QPQWRKYVNAPTGYADGLTNDQILGPLNPTLRGIALQLPAGLGENADNKYVYSALSRE
                     YGDVAVLRAKIPTTPKTYDGEPVMGGGQLRFWSMCSGNRATQTYDCLVDKDVPLASDG
                     SYTIAVSTAGNRPANATPECGVGWLPWGADAQVTLIMRNMLPAADFTQSVQAADQGTE
                     QSTLGPYYPVGTYYATVQDFENAVGCHAPAYLPSQGQAVAGDSPAGGGSMGVGLLVLG
                     AAAARRRLTRRT"
     gene            2893..>3084
                     /locus_tag="VHE37_16710"
     CDS             2893..>3084
                     /locus_tag="VHE37_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37228.1"
                     /translation="MDTQANNYRRLVVATIAESIEPQARAAKYEEPLAQALQRAGAGI
                     VAGGGSQLDAAMRVSFVDIE"
BASE COUNT          457 a         1032 c         1074 g          521 t
ORIGIN      
        1 ctctgcgcgc cgcctcacgc cgcctgcggc agcatctgcg cgatgaaggc ctggcactcc
       61 ggcttgtcca tgtaacgcgg caccgcgtag gtccagtgcg cttccgccag cgccttgtcg
      121 gtgatggcgg ggatgtcgga ctgcttgagc ttttccacct gcgtgggaat gccgaactcg
      181 gcgttgagcg cgcgcacctt ggcgatgaag gcgtcggcca gctgcgcatc gctctcccca
      241 ccttttttca gcccgctgac ttccgccagc ttggccagcc gctcgatgca gttcggcttg
      301 gagaagtcga gcacgcgcgg caggatgatg gcgttggcca gaccgtgcgg caggtggtag
      361 taggcgccga agttgtgcgc gatggcgtgc acgtagccca cgccggcggt ggtgaaggcc
      421 acgccggcct tgtacgaggc cagcgccatg ttctgccgcg cctccacgtt gctgccgtcc
      481 ttcaccgcgg tctgcaggtt ggccatgatc aggcgcgtgg cgtcgagcgc ctcggcatcg
      541 gtcatgtcgg tgtggttgcg cgagatgtac gcctccaccg cgtgggtcag cgcgtccatg
      601 ccggtggcgg aagtgatcgg cggcggcagg ccggtcatca gcgcgccgtc gagcgcggcg
      661 cccaccggca ccagccgcgg cgacatgatg gcgaacttgc gcgtggtcga cggatcggag
      721 acgacggcgg cgatggtcac ctccgagccg gtgcctgcgg tggtcggtac cacgtacagc
      781 ggcagcagct tgaacaccac gcgcatcagc ccggccatgt gcacgatctt catgaacggg
      841 ttggtggcgc gcgccgcgat caccttggcc gcatcaatcg acgaaccgcc gccgatggcc
      901 aggatcgcgg tgcagccttc cttcttcagc atcgccagcc cggtctcgat ctgctcgatg
      961 gtcgggttgg gctgcacgcc gtcgtagacc acgtagttca cgcccagctg cgtgagtttt
     1021 tcctgcagcg gcttgagcag gccgatcttg accagcatgg cgtcggtgac gatcagcagc
     1081 ttcttcaccc cggtgtcggc gatgtgctcg cacagccgca gcgacgagcc cggcccgctg
     1141 tacagctccg gcgtgcggaa ggtgagcacc ttggtcgccc acttcaggat gaactggtag
     1201 atcttgaaga tcagcacttt cagcgaaaac ggaatcatgc ggcccctccc cggtgcgcgg
     1261 gccggacgag gcggccgcgt ggattttcag gggcgcattg tctgcttttt caggtgcggc
     1321 gggtcaagcg ccgccgggcc gccgccgcgc ccagcaccag caggccgacg cccatcgagc
     1381 cgccgcctgc cgggctgtcg ccggccacgg cttgcccctg cgacggcagg taggccggcg
     1441 cgtggcagcc caccgcgttc tcgaaatcct gcaccgtcgc gtagtaggtg ccgaccggat
     1501 agtacgggcc cagcgtggac tgctcagtgc cctggtccgc cgcctgcacc gattgcgtga
     1561 agtccgcggc cggcagcatg ttgcgcatga tcagcgtcac ctgcgcatca gcgccccacg
     1621 gcagccagcc gacgccgcat tcgggcgtgg cgttggccgg gcggttgccc gcggtggaca
     1681 cggcaatggt gtagctgccg tcagaggcga gcggcacgtc cttgtcgacc aggcagtcgt
     1741 aggtctgcgt cgcgcggttg ccgctgcaca tcgaccagaa gcgcagctgg ccgccaccca
     1801 tcaccggttc gccgtcatag gttttcggcg tcgtcggaat cttcgcgcgc agcaccgcca
     1861 cgtcgccgta ctcgcgcgac agcgccgagt acacgtactt gttgtcggcg ttctcgccca
     1921 ggcccgccgg cagctgcagc gcgatgccgc gcagcgtggg attgagcggc ccgaggatct
     1981 ggtcattggt caggccgtcg gcataaccgg tcggcgcatt cacgtacttg cgccactgcg
     2041 gctgcggttg cgcgaacagg cccaccggcg gcagcgggta gtcggacagt cccgacgccg
     2101 ccagttcctc ggtgaggaag ctggtgtccg gcaccacgtc cgggcaggtg gggatcggca
     2161 cgcgcgtgcc gccgggcagc acctcggtga gcgtgggcgc cggcaccgcg ccgaagggct
     2221 gcgtgccggc atcgggcagg tagatgcggt agaccacgga gaagcccacg cgcgtgccgc
     2281 tgtcgccgat gtaatacagc gtgttcgcgt cgccgccgga agccggcacc gcctggtcga
     2341 ccacgcgcac cgtgtagttg cgcgcctgcg ccgtgcggtc ggcaccctcg atgaacggat
     2401 tgatggaacc ggcatcgggc tggatctgca cgtcgcgcag atcgctgatg gtctgcaggt
     2461 tgaggttgta ggtctgcagc gacatgtagc gcgcatgcgg aaactggccc gcgatctcga
     2521 cgtagccgcc cggcaccgcc ggcagcggcg cggccagata gcgcgtggtg atttccggga
     2581 acaggatgtt ctcgcggtcg gcggtgggct cgacgatcca ggcgcagccc ttgtcggtga
     2641 ccgccgcctg cgcggcgcca gccatgccgg ccagcaacag cagcattgcg atgcgcatcg
     2701 tgctctcccc gaacaacgtg cgtgtatgtg tgcgcgcagc atcgcgcgcc agcgccggca
     2761 cagccacggc cgcgcggaca gcgccgaact ggcgccgcga ccatttgcgc ctgcacctta
     2821 tcttgccagt gcagcggttc ggctaacatc ggtgcccagc cgcgagcggc ggccgcgtgt
     2881 gtcgggggac gcatggatac tcaagccaat aactatcggc gcctggtcgt tgccaccatt
     2941 gccgaatcga tcgaaccgca ggcgcgcgcc gccaagtacg aagagccgct ggcgcaggca
     3001 ttgcagcgcg caggcgcggg tatcgtggct ggcggcggct cgcaactgga cgccgcgatg
     3061 cgggtgtcct tcgtcgatat cgag
//
LOCUS       DASZZC010000775         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_1092147,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000775 DASZZC010000000
VERSION     DASZZC010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092147"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..2086
                     /locus_tag="VHE37_16715"
     CDS             248..2086
                     /locus_tag="VHE37_16715"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVT37229.1"
                     /translation="MKRRLPWLPLLLAALVAAEYSGVQLLSPLEHRAQDALLRADAAR
                     RAPAADIVLVQIDERSLAEMAPEFGGFPWPRSTHADLLAGILAQQPRAVVFDLMFSEP
                     SVDHADADQYFIETAAPSPVTYLPYTLIDARAADGLDIDALGTQLGYTRVAPGASAAR
                     PRMALPFAALAQTGRIGFINFLADDDGLGRRYALRARAGNWSLPSLPARVARDLGVKL
                     PDADDIVIHWRGRQPRAHISYVDLYRDVTAGHHQRAPDELRGKIVVIGAQAAGLGDLR
                     ATPLHSLHPGSDILASAIETLVSGDALRPAPAWLGALLSLAAIALVLALARLPYPLPA
                     SGAGAVACALALTAAAWIGLQRNVQLPLFTPAFFGALAFVAQAVMAYLRERAERLQTV
                     QIFSRFVDPNVAQRLALEGSAALNKEPERRVITLLFSDIRGYTTLSEKRAPAEVMALL
                     NRYFTQQVDVVFAAGGTVNKFIGDAIMAIWGAPLDAPDHAQRAVRAALAMSRAVEQFR
                     AGLGAEGAAFDIGIGVHTGEAVVGFLGSRERSLEYTAIGDAVNLASRIEGETKNQHCR
                     ILVSEATVQACGAAFDFTERAAFNAKGRTQATRLYEPVREIEGEKS"
BASE COUNT          309 a          770 c          721 g          347 t
ORIGIN      
        1 ggccggtctc gcacagcacg atatgcggcg acagggagca ggcacccgtg gcgtaataca
       61 gcttcatgga aattctccgg ctggttcagt tcagcagaca gaataatgtg ccgcgggttc
      121 gcccggcagg ccccaccgca ccgcccggaa tgatgttact aaatattgtt catttttatt
      181 ttatatagat gtttaataac acgacaggac ggggtgcggt gagcgtaaaa accggcgctg
      241 tgcggccatg aaacggcgcc tgccgtggct gccgctgctg ctggccgctc tggtggccgc
      301 ggaatattcc ggcgtgcagc tgctgtcacc gctcgaacac cgcgcgcagg acgcgctgct
      361 gcgcgccgat gcggcgcgcc gcgcacccgc cgcggacatc gtgctggtgc agattgacga
      421 acgcagcctg gcggaaatgg cgccggagtt cggcggcttc ccgtggccgc gctccaccca
      481 cgccgacctg ctcgccggca tcctcgcgca gcagccgcgc gcggtggtgt tcgacctgat
      541 gttctccgag ccgtcggtcg accacgccga tgccgatcaa tatttcatcg aaaccgccgc
      601 gccctcgccc gtcacttacc tgccgtacac gctgatcgac gcacgcgccg ccgacggcct
      661 ggacatcgac gcgctcggca cgcagctcgg ctacacgcgc gtcgcgcccg gtgcgtcggc
      721 cgcgcgcccg cgcatggcac tgccgttcgc cgcgctggcg cagaccgggc gcatcggctt
      781 catcaatttc ctggccgacg acgacgggct cggccgccgc tatgcgctgc gcgcgcgtgc
      841 cggcaactgg agcctgccgt ccctgccggc gcgcgtggcg cgcgatctgg gcgtaaaact
      901 tcccgacgcc gatgacatcg tcatccactg gcgcgggcgc cagccgcgcg cgcatatctc
      961 ctatgtcgat ctgtaccgcg acgtcaccgc gggtcatcac cagcgcgcgc ccgacgagct
     1021 gcgcggcaag atcgtggtga tcggcgcgca ggccgcgggc ctgggcgacc tgcgcgcgac
     1081 acctcttcat tcattgcatc ccggcagcga catcctggcc agcgccatcg agacgctggt
     1141 cagcggcgac gcgctgcgcc cggcgccggc ctggctcggc gcgctgctgt cgctcgccgc
     1201 catcgcgctg gtgctggcgc tggcgcggct gccttacccg ctgccggcgt cgggtgccgg
     1261 cgctgtcgcg tgcgccctcg ccctcaccgc agccgcgtgg atcggcctgc agcgcaacgt
     1321 gcagctgccg ttgttcacgc cggccttttt cggcgcgctg gctttcgtcg cgcaggcggt
     1381 gatggcctac ctgcgcgagc gcgccgagcg gctgcagacg gtgcagatct tctcgcgctt
     1441 cgtcgatccc aacgtggcgc agcgcctggc gctcgaaggc agcgcggcgc tgaacaagga
     1501 gccggagcgg cgcgtgatca cgctgctgtt ctccgacatc cgcggctaca ccacgctctc
     1561 cgagaagcgc gcgccggcgg aagtgatggc gctgctcaac cgctatttca cccagcaggt
     1621 cgacgtggtg ttcgccgccg gcggcaccgt caacaagttc atcggcgacg ccatcatggc
     1681 catctggggc gcgccgctcg acgcgccgga ccacgcgcag cgcgcggtgc gcgcggcgct
     1741 ggccatgtcg cgcgcggtcg agcagttccg cgccgggctc ggcgccgaag gcgcggcgtt
     1801 cgatatcggc atcggtgtgc acaccggcga agcggtggtc ggctttctcg gctcgcgcga
     1861 gcgctcgctc gaatacaccg cgatcggcga cgcggtgaac ctggccagcc gcatcgaggg
     1921 cgagaccaag aaccagcatt gccgcatcct ggtgtccgag gccacggtgc aggcctgcgg
     1981 cgccgcgttt gacttcaccg agcgcgcggc cttcaatgcc aagggccgca cgcaggccac
     2041 gcggctctac gagccggtcc gggaaatcga gggggaaaaa tcatgaagcg cgttcttcca
     2101 ctcacgctgt gcgcgctggc gctcgccgcc gccgcgtccg atgccgc
//
LOCUS       DASZZC010000776         6578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_123896,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000776 DASZZC010000000
VERSION     DASZZC010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123896"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VHE37_16720"
     CDS             complement(<1..887)
                     /locus_tag="VHE37_16720"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HVT37230.1"
                     /translation="MTTSKYSLLLACALLNPAWAQAAAVQADHIEAELIAERTALQPG
                     ADNWVALRLKPETGWHVYWQNPGDTGLATKLAWTLPDGITAGAIEWPYPQLQQQRDLT
                     NYGYDHETLHLVPVTVAENFGAKTATLKAQAKWLVCSDVCIPGKADLALDLPVGGEAK
                     PDPRWMEKFAQARTQLPQAPPGWEQQFSTDHGDLTLRLSGADAAFSRASKIEFFPVAN
                     DAVNHHAPQRIALDNDHTLRLTQQQSTYYVPHSTLRGVLVLHGAGDNGTRAYAIDAQP
                     GAVLPVSADGAVKKAADPG"
     gene            955..1533
                     /locus_tag="VHE37_16725"
     CDS             955..1533
                     /locus_tag="VHE37_16725"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HVT37231.1"
                     /translation="MIATLLWLCALAPIGAQAAGALRCDGGIISEGDRVADLLGACGE
                     PAYRDSWYAPTRNPHLLNPDTEEWYYNFGANQLLRVVRVRGGHVGNIGSDGYGFDQAP
                     QPPCDTGDIVPGMSKFRLLLTCGPPVTQEARTVLRALDPDDPYDLHHRGEVTPVYREE
                     WVYNFGGNALMRIVTLENGRVTDVENGRRGFD"
     gene            1502..1942
                     /locus_tag="VHE37_16730"
     CDS             1502..1942
                     /locus_tag="VHE37_16730"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HVT37232.1"
                     /translation="MSRTAAAVLISGFAVMLAACGSAPQLRSGDGRLAPCGDPNCVSS
                     QDADPARRVEPIRYEGTREGAQQLLASIISRTEGAQIVDNQPGYIHATYTSKVLGFVA
                     DVELVFPPQKFVDVRSASRTGYYDFDVNRKRVEDLRKAFNDRQP"
     gene            2013..3188
                     /locus_tag="VHE37_16735"
     CDS             2013..3188
                     /locus_tag="VHE37_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HVT37233.1"
                     /translation="MATQRAYDIVLFGATGFTGALTAEYLARHAPKTLKWALAGRNAE
                     KLKLVRARLADINPACRELPLLSADTGDAASIKNVAEAARVVITTVGPYLNYGEPLVA
                     ACAAAGTDYVDLTGEPEFVDLMWLRYHEQAQKSGARIVNCCGFDSIPHDLGARYTVRQ
                     LPEGVPLSVEGYVRAGGTFSGGTLHSAVTAMSRLREYARVAKERRAREKYPDNRKIGS
                     TRQRIRYVKAIGAWAVPFPTIDPQVVRRSAAADHRYGPEFRYGHYVQVKKLPRAAMLV
                     AGMGALAAASQLKFTRDKLLAMKAPGEGPSEEQRARSYFRVRFIGEGGGRRVVTEVSG
                     GDPGYGETSKMLAESALSLAFDARSSGGGVITPSQAMGDALTARLQAAGIQFRVIES"
     gene            3188..3562
                     /locus_tag="VHE37_16740"
     CDS             3188..3562
                     /locus_tag="VHE37_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HVT37234.1"
                     /translation="MPRIFLAIGALYGLFGVLLGAFGAHALKERLAPDLLAVWRTAVE
                     YQFYHALALMLLALLLRDAANGLMQAGGWCFAAGVLVFSGSLYALALTGVRMLGAVTP
                     VGGLLFIAGWICLLLGALKDRG"
     gene            complement(3705..4745)
                     /locus_tag="VHE37_16745"
     CDS             complement(3705..4745)
                     /locus_tag="VHE37_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT37235.1"
                     /translation="MSAKTALVVDDSKSARFALRRFLENHQFKVETAESAEEAYTMLR
                     AHRPEVIFLDHVMPGTDGFTALKHIKNDPATGSIPVVICSSNEGPQFNEDARTQGAAH
                     VLQKPPSPERLSAILSSLQKFSAELREPDPDLKFADTGKFTPDGQVVPAAAMPPPAPS
                     KVSNIREPEVAIEQAVMKALRGAIQTRVEAAAPPAPVAPAAPVAAAAPAVADAHAAGA
                     LREQMEARLKKITQELFVQMAELKGSLAHLEAQVEAGAKDKDAAAAIAPLQQKLASLE
                     QNVQQQFADLRKQLEAGLAAQAERISQVTHAARQAAAQEAHAVAERTVMSAASRISDQ
                     LSESILKAINAR"
     gene            complement(4876..5292)
                     /locus_tag="VHE37_16750"
     CDS             complement(4876..5292)
                     /locus_tag="VHE37_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37236.1"
                     /translation="MRIPALAFALASMLSACAAPAPVASPINDGGLVIGTLSYPLQAE
                     PRHALVKFSRIAPRNALTQTYLLDAAYDAGSAQAIFSGSLPSGMYVVEEALSDDARYV
                     PGALKMPFEIQPGQVTDAGNFLMEPRAALLRTTLAQ"
     gene            5402..>6578
                     /locus_tag="VHE37_16755"
     CDS             5402..>6578
                     /locus_tag="VHE37_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT37237.1"
                     /translation="MFLPSSPGPPSISAIVSRRDLDFLLYEWLDAGALTRYPSYAQHD
                     RATFEQVIDAAYTLAEEKFLPFAAKLDKNPPSFDGQRVSIIPEVKEALAAFIEGGFMS
                     SAFPLEAGGLQLPYTVAQAAAAIFGAANGAVAGYAMLTVAAANLLRVHGTDEQRERYM
                     KPLVAGRWFGTMCLSEPQAGSSLGDIRTRATAQADGTYRISGNKMWISGGEHELTENI
                     VHLVLARIDGAPAGVKGISLFIVPRFLPDAQGKPGARNGVKLVGLNHKMGNRGTVNTV
                     LSFGETPCTGFLVGKPGEGLACMFHMMNEARIGVGMAAAMTGYAGYLFSLDYAKQRPQ
                     GRPASNKNPDSPPVMLIEHTDVRRMLLQQKAYVEGALALCLYCARLVDEENAGTDDAQ
                     "
BASE COUNT         1039 a         2144 c         2305 g         1090 t
ORIGIN      
        1 aatcccgggt cggcggcttt cttcaccgcg ccgtcggccg acaccggcag caccgcgccg
       61 ggctgcgcat cgatcgcata ggcgcgtgtg ccgttgtcgc cggcgccgtg cagcaccagc
      121 acgccgcgca gcgtcgagtg cggcacgtag taggtcgact gctgctgcgt caggcgcagc
      181 gtgtggtcgt tgtccagcgc gatgcgctgc ggcgcgtggt gattcaccgc atcgttggcg
      241 acggggaaga actcgatctt cgacgcgcgg ctgaatgcgg catcggcgcc ggacaggcgc
      301 agggtcagat cgccatgatc ggtggagaac tgctgctccc agcccggcgg cgcctgcggc
      361 agctgcgtgc gagcctgcgc gaatttctcc atccagcgcg ggtcgggctt tgcttcgccg
      421 cccaccggca ggtcgagcgc caggtcggcc ttgccgggaa tgcacacgtc ggagcagacc
      481 agccacttgg cctgcgcctt cagcgtcgcc gtcttcgcgc cgaaattttc cgcgacggtg
      541 accggcacca ggtgcagggt ttcatggtca tagccgtagt tggtcagatc gcgctgctgc
      601 tgcagctgcg ggtacggcca ctcgatggca ccggcggtga tgccgtcggg caaggtccag
      661 gcgagcttgg tggccaggcc ggtgtcgccc gggttctgcc agtacacgtg ccagccggtt
      721 tcgggcttga ggcgcagtgc cacccagttg tcggcgccgg gctgcagggc ggtgcgctcg
      781 gcgatcagct cggcctcgat atggtcggcc tgcaccgcag ccgcctgcgc ccaggcagga
      841 ttcagcagcg cgcaagccag cagcagggaa tatttgcttg ttgtcatggt cggagttcgg
      901 cgacgctatg accgcgcctg gcggccgagg tttcaaggtc gaagtatgac aaggatgatt
      961 gcaacgctgc tgtggctgtg cgcgctggcg ccgatcggcg cgcaggcggc cggggcgttg
     1021 cgctgcgacg gcggcatcat cagcgaaggc gaccgcgtgg ccgacctgct cggcgcctgc
     1081 ggcgaaccgg cctaccgcga ctcctggtac gcgccgacgc gcaacccgca cctgctcaat
     1141 cccgacaccg aggagtggta ttacaacttc ggcgccaacc agctgctgcg cgtagtgcgc
     1201 gtgcgcggcg ggcatgtcgg caacatcggc agcgatggct atggtttcga ccaggcgccg
     1261 cagccgccct gcgacaccgg cgacatcgtc ccgggcatgt ccaagttccg cttgctgctg
     1321 acctgcggcc cgccggtgac gcaggaggcg cgcaccgtgc tgcgcgcgct cgatcccgac
     1381 gatccgtacg atctgcatca ccgcggcgag gtgacaccgg tgtaccgcga ggagtgggtc
     1441 tacaatttcg gcggcaacgc gctgatgcgc atcgtgacgc tggaaaacgg gagagtgacg
     1501 gatgtcgaga acggccgccg cggttttgat tagtggcttt gcggtgatgc tggcggcctg
     1561 cggcagcgcg ccgcagctgc gctcgggcga cggccgcctg gcgccttgcg gcgacccgaa
     1621 ctgcgtgtcg tcgcaggacg ctgatccggc gcggcgcgtc gagccgatcc gctacgaggg
     1681 cacgcgcgag ggcgcacagc aactgctggc cagcatcatc tcgcgcaccg agggcgcgca
     1741 gatcgtggac aaccagccgg gctacatcca cgcgacgtac acctcgaagg tgctcggttt
     1801 cgtcgccgac gtggagctgg tgttcccgcc gcagaaattc gtcgacgtgc gctcggccag
     1861 ccgcaccggc tattacgatt tcgacgtcaa ccgcaagcgc gtggaagacc tgcgcaaggc
     1921 attcaacgac cgccagccct gagcagcggc aatcccacgc ggcagtgacg gcgcgtatca
     1981 tgcgcgccgg aaaaatcatg gggaaaaatc acatggccac gcagcgtgcc tatgacatcg
     2041 tgctgttcgg cgccaccggc ttcaccggcg cgctcaccgc ggaatacctg gcgcggcatg
     2101 cgccgaaaac gctgaagtgg gcgctggccg gacgcaacgc agagaaactg aagctggtgc
     2161 gcgcgcggct ggccgatata aacccggcgt gccgcgaact gccgctgctg tccgccgaca
     2221 ccggcgatgc cgccagtatc aagaacgtgg ccgaggccgc gcgcgtggtc atcaccaccg
     2281 tcgggccgta cctcaactat ggcgagccgc tggtggccgc ctgcgcggcg gcgggcaccg
     2341 attacgtcga tctcaccggc gagccggagt tcgtcgacct gatgtggctg cgctaccacg
     2401 agcaggcgca gaagagcggg gcgcgcatcg tcaactgctg cggcttcgat tccattccgc
     2461 acgacctggg cgcgcgctac accgtgcgcc agctgcccga gggcgtgccg ctgtcggtgg
     2521 aaggctacgt gcgggccggc ggcaccttct ccggcggcac gctgcactcg gcggtcaccg
     2581 cgatgtcgcg cctgcgcgag tacgcgcgcg tggcgaagga gcgccgcgcg cgggaaaaat
     2641 atcccgacaa ccgcaagatc ggctccacgc gccagcgcat ccgctacgtg aaggccatcg
     2701 gtgcctgggc cgtgccgttt cccaccatcg atccgcaggt ggtgcggcgc agcgccgcgg
     2761 ccgaccaccg ctacggtccc gaattccgct acggccatta cgtgcaggtg aagaagctgc
     2821 cgcgcgcggc catgctggtc gccggcatgg gcgcgctggc ggcggcgtcg cagctcaagt
     2881 tcacccgcga caagctgctg gcgatgaagg caccgggcga agggccgagc gaggagcagc
     2941 gcgcgcgcag ctatttccgc gtgcgcttca tcggcgaagg cggcggccgg cgcgtggtga
     3001 ccgaagtgtc cggcggcgat cccggctacg gcgagaccag caagatgctg gcggagtcgg
     3061 cgctgagcct ggcgttcgat gcgcgcagtt ccggcggggg cgtgatcacg ccgtcgcagg
     3121 ccatgggcga tgcgctgacc gcgcgcctgc aggccgccgg catccagttc cgcgtgatcg
     3181 aaagctgatg ccgagaatct ttctcgccat tggtgcgctg tatggattgt tcggcgtgct
     3241 gctcggcgct ttcggtgcgc atgcgctgaa ggagcggctg gcgccggacc tgctcgcggt
     3301 gtggcgcacg gcggtggagt accagttcta ccacgcgctg gcgctgatgc tgctcgcgct
     3361 gctgctgcgc gacgccgcga acgggctgat gcaggccggc ggctggtgct tcgccgcggg
     3421 cgtgctggtg ttctccggca gtctctacgc gctggcgctg accggcgtgc gcatgcttgg
     3481 cgcggtcacg ccggtgggtg ggctgttgtt catcgccggc tggatctgcc tgctgctcgg
     3541 cgcgctaaag gaccgcggct aacaagcgtc ttgcggaaaa aacggctgac cgcacggctc
     3601 ctggcggtgt tataaattgt tgtttaatta ttaaaataaa caataattaa taacaggcgc
     3661 gaccggggtg cggtaacgga aaaaatcgcg gggtaaggcg cgaatcagcg cgcgttgatc
     3721 gccttgagga tcgattccga cagctggtcg gaaatgcgcg aggctgcgga catcacggtg
     3781 cgttcggcca ctgcgtgcgc ttcctgcgcc gcggcctggc gagcggcatg cgtcacctgg
     3841 ctgatgcgct cggcctgcgc ggcgagcccg gcttccagct gcttgcgcag gtcggcgaac
     3901 tgctgctgca cgttctgctc gagcgacgcc agcttctgct gcaggggcgc aatcgccgcg
     3961 gccgcatcct tgtccttggc gccggcttcc acctgcgcct ccaggtgcgc gagcgagccc
     4021 ttgagctcgg ccatctgcac gaacagctcc tgcgtgatct tcttcagccg cgcttccatc
     4081 tgctcgcgca gcgcgccagc ggcatgcgca tcggccaccg ccggcgctgc ggcggcaacc
     4141 ggcgccgccg gtgcaacggg cgctggcgga gcggcggctt ccacgcgggt ctggatggcg
     4201 ccgcgcagcg ccttcatcac cgcctgctcg atggccactt ccggctcgcg gatgttggac
     4261 accttcgacg gcgctggcgg cggcatggcc gcggccggaa cgacctggcc atcgggcgtg
     4321 aacttgccgg tgtcggcgaa cttgagatcg ggatcgggct cgcgcagctc ggcggagaat
     4381 ttctgcaggc tcgacaggat ggccgacagc cgctcggggc tgggcggttt ctgcagcacg
     4441 tgcgccgcgc cctgcgtgcg cgcgtcctcg ttgaactgcg gcccttcgtt ggatgagcag
     4501 atcaccaccg ggatggagcc ggtggccggg tcattcttga tgtgcttgag cgcggtgaag
     4561 ccatcggtgc cgggcatgac gtgatcgagg aagatcactt ccggccggtg cgcacgcagc
     4621 atggtgtagg cctcttccgc gctctccgcg gtctcgacct tgaactggtg gttctccagg
     4681 aagcggcgca gggcgaagcg ggccgacttc gagtcgtcca cgaccaacgc ggtttttgcg
     4741 ctcatctgcc tctccggccg ccagtgctga tgcggtgcgt gactctagca ccgatccgat
     4801 gggccacaaa ataagcgcgg gatcagcgca atggctgacg atcgggatgt attttctacg
     4861 gcgccgcggc gccgcttact gggccagcgt ggtgcgcagc agtgccgcgc gcggctccat
     4921 caggaaattg ccggcgtcgg tcacctggcc gggctggatc tcgaacggca tcttcagtgc
     4981 gccgggcacg tagcgcgcgt cgtccgacag cgcctcttcc accacgtaca tgccgctggg
     5041 caggctgccg gaaaagatcg cctgcgcgga gccggcatcg taggcggcgt cgagcaggta
     5101 ggtctgcgtg agcgcgttgc gcggcgcgat gcgcgagaac ttgaccagcg cgtggcgcgg
     5161 ctcggcctgc agcgggtacg agagcgtgcc gatgaccagg ccgccgtcgt tgattggcga
     5221 ggccaccggt gcaggcgctg cgcaggccga gagcatcgat gcgagggcga aagcgagagc
     5281 aggaatgcgc atggcggctc cgtgctgaaa acgtgatttt attttagata attcaatcaa
     5341 aaaacacaac atccgttcca tgcggtagca ccgattaccc gccgcaaact tataagctcc
     5401 agtgtttctg ccatcgtctc ccgggccgcc atccatatcc gccatcgtca gccgccgcga
     5461 tcttgatttc ctcctttacg aatggctcga cgccggcgcg ctcacgcgct atccgagtta
     5521 cgcgcagcat gaccgcgcca ctttcgagca ggtgatcgac gcggcctaca cgctggcgga
     5581 ggaaaaattc ctgccgtttg cggcgaagct ggacaagaac ccgccgagct tcgacggcca
     5641 gcgcgtgagc atcatccccg aggtgaagga ggccctggcc gccttcatcg agggcggctt
     5701 catgtcgagc gcgttcccgc tggaggcagg cgggctgcag ctgccgtaca cggtggcgca
     5761 ggcggcggcg gcgatcttcg gcgcggccaa cggcgcggtc gccggctacg ccatgctcac
     5821 cgtggcggcc gccaacctgc tgcgcgtgca cggcaccgac gagcagcgcg agcgctacat
     5881 gaagccgctg gtggccggac gctggttcgg caccatgtgc ctgtcggagc cgcaggccgg
     5941 cagctcgctc ggcgacatcc gcacgcgcgc cacggcgcag gccgacggta cttaccgcat
     6001 cagcggcaac aagatgtgga tctcgggcgg cgaacatgag ctgaccgaga acatcgtgca
     6061 cctggtgctc gcgcgcattg acggcgcgcc ggcgggcgtg aagggcatct cgctgttcat
     6121 cgtgccgcgc ttcctgcccg atgcgcaggg caaaccgggg gcgcgaaacg gcgtgaagct
     6181 ggtcggcctc aaccacaaga tgggcaaccg cggcacggtc aacaccgtgc tgtcgttcgg
     6241 cgagacgccg tgtacaggat ttctcgtcgg caagccgggc gaaggcctgg cctgcatgtt
     6301 ccacatgatg aacgaggcgc gcatcggcgt cggcatggcg gcggcgatga ccggctatgc
     6361 gggctatctc ttctcgctgg actacgcgaa gcagcgcccg cagggccggc cggcgtcgaa
     6421 caagaatccc gacagtccgc cggtgatgct gatcgagcac accgacgtgc gccgcatgct
     6481 gctgcagcag aaggcctacg ttgaaggcgc gctggcgctg tgcctgtatt gcgcgcgcct
     6541 ggtcgacgag gagaacgccg gcaccgacga cgcgcagc
//
LOCUS       DASZZC010000777         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_189604,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000777 DASZZC010000000
VERSION     DASZZC010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189604"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2199
                     /locus_tag="VHE37_16760"
     CDS             <1..2199
                     /locus_tag="VHE37_16760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT37238.1"
                     /translation="ELLAALEHPGIARMYSAGVAQTGAGELPYLAMEYVRGADLLTHA
                     QERALTTRQKLELVIAIARAVHHAHTRGAIHRDLKPANILVDEHGQPKILDFGVAHVT
                     GRDDVTQMTVAGEILGTVPYMPPEQLGGQARAVDPSWDVYALGVIAYELLCGELPYPG
                     MSKATVLTALQEKLSKAPVRLSKKLPQARGDLETIIDKAMASEAAQRYSSAAELAADL
                     ERFLESRPIEARPPTMRYVLGLFVRRHRAFTAAAMLVFAALIVATAVSAHFAYSASQR
                     ASEREAVNQFMQDMFAAADPERSLGEHLTVRDVLDVARSELQGGHALAPGATAQVQRT
                     LANTYVNLGLAQQGIELLRSAQSASDELYGADSDEALQVRIELARALTEAGQEAEGEK
                     LLQPMIAALAQAQGTRRRLLLEAQMQQADILVNQGKAADAEKLLRASLPQLEQVFGAD
                     DDLTLHARYNLAQVLHLQARYDESIAQAQLVVQKETAKRGAEHPRVQLARDVIAETYR
                     EQAKYAQAEAIYRDTVALREKVLGTDHPQTNVARVSLAAVLAAEGRAGEAAPIARQAH
                     EGILRYLGPEAELTRNVASLRAYVVSEAGDWQEAAQINRELVTQDEERPSGPTETDLP
                     DYNNLGNALKHTGRLDEAIAVYRKLLGYAEKIVGRQHLHYGLFELNYADGLIEQKRLG
                     EARPLLEHSLAILRKELGDDHPKTKKAIDRLRAVYTRLGLKAQAAALPAA"
BASE COUNT          391 a          753 c          795 g          291 t
ORIGIN      
        1 gagctgctgg ccgcgctgga gcatcccggc attgcgcgca tgtacagcgc cggcgtggcg
       61 cagaccggcg cgggcgagtt gccgtacctg gcgatggaat acgtgcgcgg cgccgacctg
      121 ctcacgcacg cgcaggagcg cgcgctgacc acgcggcaga aactggaact ggtcatcgcc
      181 atcgcgcgcg cggtgcacca cgcgcatacg cgcggcgcca tccaccgcga tctgaagccg
      241 gccaacatcc tggtcgacga gcacggacag ccgaagatcc tcgacttcgg cgtggcccac
      301 gtgaccgggc gcgatgacgt cacccagatg accgtcgccg gtgaaatcct cggcactgtt
      361 ccgtacatgc cgcccgagca gctgggcggg caggcgcgcg cggtggaccc gtcgtgggac
      421 gtgtatgcgc tcggcgtcat cgcctacgaa ctgctgtgcg gcgagctgcc gtatccgggc
      481 atgtccaagg ccaccgtgct caccgcgctg caggagaagc tgtcgaaggc gccggtgcgc
      541 ctgtcgaaga aactgccgca ggcgcgcggc gacctggaaa ccatcatcga caaggcgatg
      601 gcgtcggaag cggcgcagcg ctacagctcg gccgcggaat tagccgccga tctcgaacgc
      661 ttcctggaga gccggccgat cgaggcgcgg ccgccgacca tgcgctacgt gctcggcctg
      721 ttcgtgcggc gtcaccgcgc tttcaccgcg gcggccatgc tggtgttcgc cgcgctgatc
      781 gtcgccaccg cggtgtcggc gcacttcgcc tacagcgcca gccagcgcgc cagcgagcgc
      841 gaggcggtca accagttcat gcaggacatg ttcgccgccg ccgaccccga acgttcgctg
      901 ggcgagcacc tgaccgtgcg cgatgtgctc gacgtggcgc gcagcgaatt gcagggcggc
      961 cacgcgctgg cgccgggcgc gacggcgcag gtgcagcgca cgctggccaa cacctacgtg
     1021 aatctcgggc tggcgcagca gggcatcgag ctgctgcgct cggcgcagtc ggcatcggac
     1081 gaactttatg gtgcggattc cgatgaagcg ctgcaggtgc gcatcgagct ggcgcgcgcg
     1141 ctgaccgaag cggggcagga agccgagggc gagaagctgc tgcagccgat gatcgcagcg
     1201 ctggcgcagg cccagggcac gcgccgccgt ttgctgctgg aagcgcagat gcagcaggcc
     1261 gacatcctgg tcaaccaggg caaggcggcg gatgcggaga agctgctgcg cgcgagcctg
     1321 ccgcagctcg agcaggtgtt cggtgccgac gatgacctga ccctgcacgc gcgctacaac
     1381 ctggcgcagg tgctgcacct gcaggcgcgc tacgacgaat cgatcgcgca ggcgcaattg
     1441 gtcgtgcaga aggaaaccgc caagcgcggc gccgagcatc cgcgcgtgca gctggcgcgc
     1501 gacgtcatcg ccgagaccta ccgcgagcag gccaagtacg cgcaggccga ggcgatctac
     1561 cgcgacacgg tggcgctgcg cgagaaggtg ctgggtaccg accatccgca gaccaacgtg
     1621 gcgcgcgtca gcctggcggc ggtgctcgcg gccgagggcc gcgcggggga agcggcgccc
     1681 atcgcgcggc aggcgcacga gggcatcctg cgctacctgg ggcccgaggc cgagctcacg
     1741 cgcaacgtcg ccagcctgcg cgcgtacgtg gtgagcgagg ccggcgactg gcaggaagcg
     1801 gcgcagatca accgtgagct ggtcacgcag gacgaggagc ggccgagcgg ccctaccgaa
     1861 accgatctgc ccgactacaa caacctgggc aacgcgctca agcacaccgg caggctcgac
     1921 gaagccatcg cggtatatcg caagctgctc ggctacgcgg aaaaaatcgt cggcaggcag
     1981 cacctgcact acggcttgtt cgagctgaat tacgccgacg ggctgatcga gcagaagcgc
     2041 ctgggcgaag cgcgtccgct gctggaacac agcctggcca tcctgcgcaa ggagctgggc
     2101 gacgaccatc cgaaaacaaa gaaggcgatc gatcgcctgc gcgcggtcta cacgcgcctg
     2161 ggcctgaagg cgcaggccgc tgcgctgccg gcggcatgat cggcttcacc ctcttcgtcg
     2221 cgggcatcgg
//
LOCUS       DASZZC010000778         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_189667,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000778 DASZZC010000000
VERSION     DASZZC010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189667"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHE37_16765"
     CDS             <1..265
                     /locus_tag="VHE37_16765"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVT37239.1"
                     /translation="LHTDTEGHDYEIIKLIDFGRFRPSLLVYEHHHLTPPDRVACAAH
                     MEALGYETMTEGLDTMCLDVRNCNEELRRVWQLLRGIEGPVPA"
     gene            262..1269
                     /locus_tag="VHE37_16770"
     CDS             262..1269
                     /locus_tag="VHE37_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37240.1"
                     /translation="MNPGGSTATDGRARAQHRRFPRLRARFEKRYARERDARFIEMDI
                     ARFPYMGFGATIGWIHAVYVACDMLGARLLVLNPSRWPFGGNTADGAIDRFLEFPEAD
                     LRQASTPGALPYQIGSWDNLRCWGYYDPLDLPTCTFGYRPPRFATLDDYRRDLMRRMY
                     RPTAFVRGELDRALDFCPSRYIAWHARRGDKTSGVWKEDEAVPLSRYCEETRAILQRD
                     PAAPRVVVLCSDSPLVLDEARDEARRMGLEVEFIADPAEKRWDGYCALQRTGKITSTA
                     EVVAEVLTAQKNIEVMRAAEYLLGCNSSCLFRVAALLRADGDTVTSFSENKVYKKYYP
                     I"
     gene            1278..2195
                     /locus_tag="VHE37_16775"
     CDS             1278..2195
                     /locus_tag="VHE37_16775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT37241.1"
                     /translation="MSAAPLVSIIVPSYNQGRFLRETLDSILAQDHRPIEILVMDGAS
                     TDDSVAILQEYAARHAELQFWSEPDKGPADAVNKGLARARGVYAGIQSSDDLYRPGAI
                     SAAVRAFDQRPGTGVVYADTEIIDEHGRLLLLAPSRLPYSQARLFSRSTVIHQSSAFF
                     RPELARELGGWNGRYFCCDTDLWLRLSFVTGIRRVDGVWSAWRKHAGQRDREARKMWD
                     SWGRMIDECEPLRRASWHLRLAASAGRRLVALDYSPSRKPGFRFMQAWLAVLTWPPSL
                     AGIYSKSVLVPGLGRLLARHSSQATTAHG"
     gene            2188..3234
                     /locus_tag="VHE37_16780"
     CDS             2188..3234
                     /locus_tag="VHE37_16780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar O-methyltransferase"
                     /protein_id="HVT37242.1"
                     /translation="MAETKSPPGPLQRLKGGMRRLLRRSGYDLVRVAPRDTRYAGASY
                     DTSRPLPAGASEALRPDHPHLLDLRQRYRDCGAPMVPRAMWAPDYLKRELDLVHFRGD
                     NPYVWQFRNVGALAKHKYYMYLRDLASRDAHGLLGKLGEDGLFGCWTFDYPGWPTVSR
                     DLLDSVNELYFLDRHLQLLSRQDFNILDIGAGYGRMAHRVLAAAPGVSHYLCVDAVPE
                     STFLCEYYTAFHSLQPRAEVIALDRLEQGLAGRRIDLAVNVHSFSEMTTPNIQGWLDW
                     LVRLQVPWLFIVPNDADRLLTMEDDGRRGEFASLLQRAGYRQVVCEPVFADPTMREFI
                     NVTDHFFLFRREAA"
     gene            3231..>3904
                     /locus_tag="VHE37_16785"
     CDS             3231..>3904
                     /locus_tag="VHE37_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HVT37243.1"
                     /translation="MSLRPLLAAGLGLAALLLQPLARADDAPVNCPLDVSAAAVLPGV
                     PLAHLAGGRLLVGSAANSFAPWSDLRYRSVLAQQFAMLVPENEMKWKFIHPQQDRYDF
                     RYADCLVHFAQQHGMKVMGGPLVWHFQQSPWLDTLDLDHDGYVALLRDHIRSVIGHFR
                     ERFPGVVTQWEVVNEPISNETCAKTLKCGLRSYFWSRKLGAEYINLAFKFAREADPQA
                     QLLLNET"
BASE COUNT          610 a         1347 c         1287 g          660 t
ORIGIN      
        1 gctgcacacc gataccgagg ggcatgatta cgagatcatc aagctgatcg acttcggccg
       61 tttccgcccc tcgctgctgg tatacgagca tcatcacctg acgccgcccg atcgcgtcgc
      121 ctgcgccgcg cacatggagg cgctgggcta cgagacgatg accgaaggcc tcgacaccat
      181 gtgcctggac gtgcgcaatt gcaatgaaga actgcgccgg gtctggcagt tgttgcgcgg
      241 catcgaaggc ccggttccgg catgaacccg ggcgggtcca ccgctaccga tggccgcgcg
      301 cgcgcgcagc atcgccgatt tccccggctg cgcgcgcgct tcgagaagcg ctacgcgcgc
      361 gagcgcgacg ccaggttcat cgaaatggat atcgcgcggt ttccctacat gggcttcggc
      421 gccaccatcg gctggatcca tgcggtctac gtcgcctgcg acatgctcgg ggcgcgcctg
      481 ctggtgctca acccgtcgcg ctggcctttt ggcggcaata ccgcggacgg tgcgatcgat
      541 cgcttcctcg aatttcccga ggccgatctg cgccaggcga gcacgcccgg tgcgctgccg
      601 taccagatcg gcagctggga caacctgcgc tgctggggct attacgatcc gctcgacctg
      661 ccgacctgca ccttcggtta tcgtccgccg cgcttcgcca cgctggacga ttaccgccgc
      721 gacctgatgc gccgcatgta ccggccgaca gcgttcgtcc gcggcgaact ggaccgcgcg
      781 ctcgatttct gtccatcgcg ctacatcgcc tggcatgcgc ggcgcggcga caagacctct
      841 ggcgtgtgga aggaagatga ggcggtgccg ctgtcgcgtt attgcgagga gacgcgcgcg
      901 atcctgcagc gtgatcccgc cgcgccgcgc gtggtggtgt tgtgcagtga ttcgccgctg
      961 gtgctcgacg aagcgcgcga cgaagcccgg cgcatgggcc tggaggtgga gttcatcgcc
     1021 gatccggcgg agaaacgctg ggacggctac tgcgctctgc agcgtaccgg caagatcacc
     1081 agcacggccg aggtggtggc cgaggtgctg accgcgcaga agaacatcga ggtgatgcgt
     1141 gcagccgaat acctgctggg ctgcaacagt tcctgcctgt tccgggtggc ggcgctgctg
     1201 cgcgccgacg gcgataccgt caccagcttc tccgagaaca aggtctacaa gaagtactac
     1261 ccgatctgat cccggacatg agcgctgcgc cgctggtctc catcatcgtg ccgagctaca
     1321 accagggacg tttcctgcgc gagacgctgg actcgatcct ggcccaggac caccggccga
     1381 tcgagatcct ggtgatggat ggcgcttcca ccgacgacag cgtcgccatc ctgcaggaat
     1441 acgcggcacg ccacgccgaa ctgcagttct ggtcggaacc cgacaagggg ccggccgacg
     1501 ccgtgaacaa aggcctggcg cgcgcccgcg gtgtctatgc aggcatccag agttccgatg
     1561 acctgtatcg tccgggcgcc atcagcgcag cagtgcgcgc cttcgaccag cggcccggca
     1621 ccggcgtggt ctacgccgac accgagatca tcgacgagca cggccgtctg ctgctgctgg
     1681 cgccgagccg cctgccgtac tcgcaggcgc ggctgttctc gcgcagcacg gtcatccacc
     1741 agagcagcgc gttcttccgt cccgaactgg cgcgcgaact cggcggctgg aacgggcgct
     1801 acttctgctg cgacaccgac ctgtggctgc gcctgtcctt cgtcaccggc atccgccgcg
     1861 tcgacggcgt gtggtcggcc tggcgcaagc atgcgggcca gcgtgaccgc gaggcgcgca
     1921 agatgtggga ctcgtggggt cgcatgatcg acgaatgcga accgctgcgc cgcgcatcgt
     1981 ggcacctgcg cctggcggcc agcgcagggc gccggctggt ggcgctggat tacagcccgt
     2041 cgcgcaagcc cggcttccgc ttcatgcagg cctggctggc cgtgctcacc tggccgccga
     2101 gcctcgctgg catctacagc aagagcgtgc tggtaccggg cctcgggcgt ttgctggcgc
     2161 gacattcctc ccaggcgacc actgcccatg gctgaaacca aatcaccacc cggtccgctg
     2221 cagcgcctga agggcggcat gcgcaggctg ctgcgccgca gcggctacga cctggtgcgc
     2281 gtcgcgccgc gcgatacccg ctacgccggc gccagctacg acacttcgcg cccgctgcct
     2341 gccggcgcca gcgaggcgct gcgtccggat catccgcacc tgctcgacct gcgccagcgc
     2401 taccgcgact gcggcgcgcc gatggtgccg cgcgccatgt gggcgccgga ttacctgaaa
     2461 cgcgaactgg acctggtgca tttccgcggc gacaacccat acgtctggca attccgcaac
     2521 gtcggggcgc tggccaagca caagtactac atgtacctgc gcgacctggc ctcgcgcgat
     2581 gcgcacgggc tgctcggcaa gctcggtgaa gacgggctgt tcggctgctg gaccttcgac
     2641 tatcccggct ggcccacggt gagccgcgac ctgctcgact cggtcaacga actttatttt
     2701 ctggatcgcc acctgcagct gctgtcacgg caggacttca acatcctcga catcggcgcg
     2761 ggctacggcc gcatggcgca ccgcgtgctg gccgcggcac cgggcgtcag tcattacctg
     2821 tgcgtcgacg cggtgccgga gtccacgttc ctgtgcgagt actacaccgc gttccactcg
     2881 ctgcagccgc gcgcggaagt catcgcgctg gaccggcttg agcagggact cgccgggcgg
     2941 cgcatcgacc tcgcggtcaa cgtgcacagc ttctccgaga tgaccacgcc caacatccag
     3001 ggctggctcg actggctggt gcggctgcag gtgccgtggc tgttcatcgt gcccaacgat
     3061 gccgaccgcc tgctgacaat ggaggacgac ggccggcgcg gtgaatttgc ctcgctgctg
     3121 cagcgcgccg gctaccggca ggtggtgtgc gagccggtgt tcgccgatcc gaccatgcgc
     3181 gagttcatca acgtgaccga tcacttcttc ctgttccggc gggaggccgc gtgagcctgc
     3241 ggccgctgct ggcagcgggc ctgggcctgg cggcgctgct gctccagccg ctggccaggg
     3301 cggacgacgc gccggtcaac tgcccgctcg atgtctcggc tgcagcagtg ctgcccggtg
     3361 tgccgctggc gcacctggcc ggcggccgcc tgctggtggg cagcgcggcg aacagttttg
     3421 cgccgtggag cgacctgcgt taccgcagcg tgctggccca gcagttcgcc atgctggtgc
     3481 cggaaaacga gatgaagtgg aagttcatcc acccgcagca ggaccgctac gatttccgct
     3541 acgccgactg cctggtgcac ttcgcccagc agcacggcat gaaggtgatg ggcggtccgc
     3601 tggtctggca cttccagcag tcgccctggc tcgacacgct cgacctggac catgacggct
     3661 acgtcgccct gctgcgcgat cacatccgca gcgtcatcgg ccatttccgc gagcgtttcc
     3721 ccggcgtggt cacgcagtgg gaagtggtca acgagccgat ctccaacgag acctgcgcca
     3781 agacgctcaa gtgcgggctg cgcagttact tctggtcgcg gaaactcggc gccgagtaca
     3841 tcaacctggc cttcaagttc gcccgcgagg ccgatccgca ggcgcagctg ctgctcaacg
     3901 aaac
//
LOCUS       DASZZC010000779        13982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_636159,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000779 DASZZC010000000
VERSION     DASZZC010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 13982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13982
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636159"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VHE37_16790"
     CDS             complement(<1..424)
                     /locus_tag="VHE37_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT37244.1"
                     /translation="MNSSIQPALAALNQVVLGKPQQVRLALACLLARGHLLIEDIPGM
                     GKTTLALAIARVLGLKFQRVQFTSDLLPADILGVSIYEQQTGTFRFHPGPVFAQLVLA
                     DEVNRASPKTQSALLEAMEERQVTAEGQTRALPEPFFVI"
     gene            579..2066
                     /locus_tag="VHE37_16795"
     CDS             579..2066
                     /locus_tag="VHE37_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HVT37245.1"
                     /translation="MDRVARECEREMEHAVTYAAWRDAAAELDRREGLDDWKADEISK
                     DYDWRLIRSRLRQIRQYRQENDVARLVYHLRQGLHWNLGNTGNPELYQHARIGTKNLV
                     REYVTEVSDALNHLCELEAAEFPHHEKLKFFHETALSYGRSALMLSGGATLGLFHVGV
                     VKALFREGLLPTVLSGSSAGSVVAATVGTRAEHEAEDLLDPTNAYYHFWRVLPPKHML
                     ARRSIMDQTQLRRAIAKNIKDMTFEEAYKLSGRIVNITVSPAGANQPPKLLNYLTFPH
                     LYLREAVLASCAVPVLFPPVMLMTRDIGGERVPYMPLLRWNDGSLKSDLPTIRLRRLH
                     NVNHFIVSQTNPHVLPFMTQRDPGARGVFHATRTFAFSTLKHTAKNVLDLARANLSGA
                     VRGTLDTASAILDQDYRGNINILPEVSLWRYINVTRNPTLESVQRFMLEGERSTWPRM
                     EMIRNQTLISQTLDRCVDRLEHTSHNMNVCDLPKKPGLRVVRNRS"
     gene            complement(2222..3487)
                     /locus_tag="VHE37_16800"
     CDS             complement(2222..3487)
                     /locus_tag="VHE37_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37246.1"
                     /translation="MKRAVGLALFIAAGSAQADISCKEGETPVDQLPVAIATGIAGAT
                     LTASAPRSTPTETKSGAAAGSAASAHITDGADFPSLLAFAVENGFASENAGATTFNLN
                     LFAIEAFLDPKALDDQYRYEQYQTLRRFGGSVSFGGKGQSFDRDGDGKPDAAETAANS
                     SDIVSWEFTYRFYGSRDRRDPQNFERFFARSAQGNAQLDQLYAPTMLQTSKSLKAMGH
                     DMHSSCVTAEERLKLASDAALVEKYRSVAAAADMVDSAANEILQEIDQGTLWTLYING
                     VSRKPQFGNDEYGAGIRGGWGGPDRGLTLNAGYRQVQDGPAGPLNRSAIGALQFSQLV
                     MRKNALSKEGMKLSLSARLEKYWDHAPVHGTIAKLNAQLEIPVSDAVTIPLSVTWANH
                     TDLVTGEKDVRGNIGISFNPSELFKPTKL"
     gene            complement(3484..3873)
                     /locus_tag="VHE37_16805"
     CDS             complement(3484..3873)
                     /locus_tag="VHE37_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37247.1"
                     /translation="MTICSDGPQDLTPLEVDCKSTDTCTWKYSVCMPNASISLYLTSA
                     ADPGHLQPLLQNVQKKNGDVLTHDVIFPGPGTYELSWSTVTADAAWANKVELLVNTAV
                     QYLLRKSNASAGSDALNKRGAVMLEVT"
     gene            complement(4020..7088)
                     /locus_tag="VHE37_16810"
     CDS             complement(4020..7088)
                     /locus_tag="VHE37_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HVT37248.1"
                     /translation="MQYAELHCLSNFSFLRGASHADELVARARALGYAAIAITDECSM
                     AGMVRAHDAAKTAGIQLLVGSEFRLACGLHLVLLAPDHDAYTQICRLITRGRRAAGKG
                     HYQLAREDFVRGLDRCLGILVLGAATDDAQVQWLHGADLAGRWIGVAHTFAQDSDERV
                     EHARRLARAHGLRAVALGSACYHVRARRPLHDVLTATREKCTVAALGKRRLANGERHL
                     RPLSTLQKLFPPEMLEETVLIAQRCTFSLDLLRYQYPHELVPEGLSATEHLRRLTYEG
                     ARRRWPAGIPGRELGLIEKELKLIAELEYESFFLTVEDIVRWARSRNILCQGRGSSAN
                     SVVCYALGVTAVDPKRLGMLFERFISRERAEPPDIDIDFEHQRREEVMQYIYEKYGRD
                     RAALAATVIRYRPRSAIRDVGKALGFSLDQIDQLAKSLFWRDGADAIEPQLRALGFDP
                     HSPIVRRLMTLVMDLLGFPRHLSQHVGGFVISEHALDALVPVENAAMPDRTIIQWDKD
                     DLESLKMLKVDCLALGMLSALRRMLEMVGQQRGSVVALTDIPDEDEKTYDMLCRGESV
                     GVFQVESRAQMSMLPRLQPRTYYDLVVQVAIVRPGPIQGGMVHPYLKRRAGIEPVFFP
                     KPELEKVLGRTFGVPIFQEQVMQIAMVAANFSAGEADQVRRSMAAWQKRGGLEHFRDR
                     LLAGMAANGYEPEFAESIYQQILGFGSYGFPESHSASFALLVYASAWLKCHEPAIFCA
                     ALINSQPMGFYAPAQLVADARRNGVRFLPVDVTLSDWDCTLQPGSGEQPDVRLGLRVV
                     NGISEGEARKLVQARARAPYSGIDDLKHRSQIGTRALHALARADALRSLAGHRHRAQW
                     GALGVENLPGFLAGHAAREMPAVLPAPSEGQDVLADYRATGLTLRRHPLEILRPRLDR
                     LEVIRSDRLRDLSSGITVRVAGIVTHRQMPETATGIMFVSLEDEAGIANLVLWNSVHR
                     AQRRPVLGARLMLVTGELQHEQGVINVLAQRVEDYSAWLGALPAASRDFR"
     gene            complement(7140..8543)
                     /locus_tag="VHE37_16815"
     CDS             complement(7140..8543)
                     /locus_tag="VHE37_16815"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HVT37249.1"
                     /translation="MLWLCIHLPELPLEALASSENLAAAIEHEGSRRHVLSCTVAATE
                     AGITVGLDAASAQALAPQIKLLERSPRRERETLRALAGWAYQYSSTISAHTTRRLLWI
                     EVGTSLKLFGGLARLQQLIASGIEALGYRAQIGVAPTLEAAALCALLAPQPPVTEKKQ
                     ITARIEKFPISALALPDDVLQGLQASGLRRIGEIYALGYDELARRFSRDTTEYLRRLI
                     GMRPETMRPYRPPETYQRQIEFNAAIETTEGLGFSLRRMLGEFQGYLRGRDTGVQQFE
                     LVLEHEHVPPTTLQVKTSRPQRDAAQLHSLLREQLNRSPIAAPVIRLRLQASSFADPG
                     DTQLDFFDGHKRQDEEWFGLLDKLSARLGREAVRTLGLQSDHRPEKAWCVLRDGPAPE
                     IPGPLPERPLWLLPQPKQVDVLPLLTSRPERIEDGWWEGDDSRRDYYTAQTRDGSRLW
                     LYRDGHNLRWYLHGVWA"
     gene            complement(8553..9368)
                     /gene="imuA"
                     /locus_tag="VHE37_16820"
     CDS             complement(8553..9368)
                     /gene="imuA"
                     /locus_tag="VHE37_16820"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HVT37250.1"
                     /translation="MSRAQAIVRELGDRRLWTGHSIAKIAAEPTGFAALDQVLPGHGW
                     PLGALTELIPQVEGIGELSLTLPALARLSARGKRIAFIAPPYIPYAPALVRAGIAPER
                     VLWLPELAPDDAGWSAVQMLKSGAVGAVLLWTRRLSDTELRRIQLGAENGSALCFCYR
                     PAPALKQASIASVRLALRPGVGGLRVDVQKAKGGRQGADVLCPLRERASLFHVETASS
                     SSPTIALLASPPGRGRAAGAGEGRTHSQHPSCIRPTALTPAPLPVGEGKSNIH"
     gene            complement(9384..10004)
                     /gene="lexA"
                     /locus_tag="VHE37_16825"
     CDS             complement(9384..10004)
                     /gene="lexA"
                     /locus_tag="VHE37_16825"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVT37251.1"
                     /translation="MDELTPRQAEVLRFIRQFAVQHGMAPSRADIAEAFKVTRKAAHD
                     WLHILAKKGELTLHPGRARGVRLPPVKPMRDPTLLPLVGRVAAGPPVLSTDEVDDWLR
                     IDPALFHPRPDYLRKVHGDSMIEMGIQDGDLLAVHAQPDADSGQVIVAKLFRDGEPEI
                     TVKKYRRRGHHVQLLPRNAALAPIDIDLTQEEFEIEGLYCGHIHRA"
     gene            complement(10102..10296)
                     /locus_tag="VHE37_16830"
     CDS             complement(10102..10296)
                     /locus_tag="VHE37_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37252.1"
                     /translation="MLVILRFRRPITAGAALSALSAAADKWAGGNPQWMPSGAPKKSH
                     RTGISTVINHLMNVILHDDY"
     gene            10319..10621
                     /locus_tag="VHE37_16835"
     CDS             10319..10621
                     /locus_tag="VHE37_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37253.1"
                     /translation="MSSTTTQNAVPTIVPTRPSTQWPVGVRLRFKPDAELHAQHATLK
                     GTQVLVLSGLKLIGPSGPDLRCSWRQEILSFAAGCRVGWARPDQLELPLDEEEDAI"
     gene            10733..12115
                     /gene="hemN"
                     /locus_tag="VHE37_16840"
     CDS             10733..12115
                     /gene="hemN"
                     /locus_tag="VHE37_16840"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HVT37254.1"
                     /translation="MDTAAFDADLVRRYDRPGPRYTSYPTALQFREDFGPEQYREAIA
                     RSEIAGGPLSVYVHVPFCANPCYYCGCTRIITRQPAVVSKYLARIIDEIRLQSLRFGP
                     QRPVEQLHLGGGTPTYFSAEQLAELMEALAANFNLRHDARREFSIEMDPRTAQPGLIQ
                     ALAAMGFNRLSLGVQDFSPDVQRAVNRVQTVDDTAALIAEARKAGFTSVSVDLIYGLP
                     LQTLQSFGRTLAQVASLRPDRIAAYSYAHLPERFKAQRQINSADLPDAETKLALLGLT
                     VNVLTMAGYQYIGMDHFALPEDELAVAQRNGTLHRSFQGYSTHGGLDLIGLGLSSIGR
                     VADTYSQNFKTLDRYYGALDAWELPVERGLRLSADDRARRSVIESIMCQGRVDFHAIE
                     REHRIEFNSYFRESLRQLEPLQQDGLVRLSAHHLEVTPRGCLLLRAVAMPFDAYAAPG
                     VVQQRYSKMI"
     gene            complement(12387..13415)
                     /locus_tag="VHE37_16845"
     CDS             complement(12387..13415)
                     /locus_tag="VHE37_16845"
                     /inference="COORDINATES: protein motif:HMM:NF023168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HVT37255.1"
                     /translation="MPAGVTIEDVTRAADAVLAKGERPTIDSVRAHLGTGSPNTVNPL
                     LKQYFKSLPDRLNLPAPIAIAAAELYKKVRDAAQEDVDKRDAEQQAALKLERDQVQAE
                     RNAFETERAQLRSATTALETELAGLKERHTALERQLVAAQAEAASQARNASAAEAKAQ
                     AATDERERASRKHQEEIASQKDRAEGNERHFLGQIDDLKTQLKKLGGDREKEQATAAR
                     RIADLESKGAELVTEIAEQRKEVIRLNADLSREQRSRATAESDLKTKVEAASREIDAA
                     NGQRAAALAERDAERLLVEQLRRERDAAMRDAAVQQGRTEALQAQLERLHAKKLSSKT
                     VQQGLLEK"
     gene            13630..>13982
                     /locus_tag="VHE37_16850"
     CDS             13630..>13982
                     /locus_tag="VHE37_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37256.1"
                     /translation="MPANLEAQVEAAVDAFRAAATSPATWESYSGALRYVCAWAALRF
                     GKPLEFPVPASVAKLFVIDHFGQPERINAGSGRTKLMLELTLPAAVDQALVDGGFKAK
                     LGRLKMSTIDHRLWVL"
BASE COUNT         2340 a         4578 c         4542 g         2522 t
ORIGIN      
        1 cgatgacgaa gaacggttcg ggcagcgcgc gcgtctggcc ttcggcggtc acctggcgtt
       61 cttccatggc ctcgagcagc gccgactgcg tcttcggact cgcacgattc acctcgtcgg
      121 ccagcaccag ctgcgcgaac accgggccgg ggtgaaagcg gaacgtaccg gtctgctgct
      181 cgtagatgga cacaccgagg atgtccgccg gcagcaggtc ggaggtgaac tgcacgcgct
      241 ggaacttgag gccgagcacg cgcgcaatgg ccagcgccag cgtggtcttg cccatgccgg
      301 ggatgtcttc aatcagcagg tggccgcgcg cgagcagaca ggcgagcgcc aggcggacct
      361 gctgcggctt gcccagcacc acctggttga gggcggccag ggctggctgg atggatgagt
      421 tcatcgtcag gttgtcgtta taagtgtgga gcgttcagcg tgcgcgatgc gccgggcatt
      481 attgcgcagc gtgccgcagt gctgcttagg ggaaaaagtc ctttgcttgt tagtgacgca
      541 tgcgtcacga tactgcaata caagattcgg agtcgtccgt ggatcgagtg gcgcgcgaat
      601 gcgaacgcga gatggaacat gcggtgacct atgccgcgtg gcgcgatgcc gccgcggaac
      661 tggatcgccg ggaaggactg gacgactgga aggccgatga aatctccaag gactacgact
      721 ggcgcctgat ccgctcgcgc ctgcgccaga tccgccagta ccgccaggaa aacgatgtcg
      781 cgcggctggt ctaccacctg cgccagggcc tgcactggaa cctgggtaac accggcaacc
      841 cggagctgta ccagcacgcg cgcatcggca ccaagaacct ggtgcgcgaa tacgtgaccg
      901 aagtttccga cgcgctgaat cacctgtgcg aactcgaagc cgcggaattt ccgcatcacg
      961 aaaagctcaa gttcttccac gaaaccgcgc tgtcgtacgg gcgctcggcg ctgatgcttt
     1021 caggcggtgc cacgctgggc ctgttccacg tgggcgtggt caaggcgctg ttccgcgaag
     1081 gcctgctgcc gacggtttta tcgggatcgt cggctggctc ggtcgtcgcc gccaccgtcg
     1141 gcacgcgggc ggagcatgaa gccgaggatc tgctcgatcc cactaacgcc tactaccatt
     1201 tctggcgcgt gctgccgccc aagcacatgc tggcgcggcg ctcgatcatg gaccagacac
     1261 agctgcgccg ggccatcgcc aagaacatca aggacatgac gttcgaggag gcgtacaagc
     1321 tgtccggacg catcgtcaac atcaccgtct cgccggccgg cgccaaccag ccgccgaaac
     1381 tgctcaacta cctgaccttc ccgcacctgt acctgcgcga agcggtgctg gcgtcgtgcg
     1441 cggtgccggt gctgttcccg ccggtgatgc tgatgacgcg cgacatcggc ggcgagcgcg
     1501 tgccgtacat gccgctgctg cgctggaacg acggctcgct caagagcgac ctgccgacga
     1561 tcaggctgcg ccggctgcac aacgtcaacc acttcatcgt cagccagacc aatccgcacg
     1621 tgctgccgtt catgacgcag cgcgaccccg gcgcgcgcgg cgtgttccat gccacgcgca
     1681 cgttcgcctt ctccacgctc aagcacacgg cgaagaatgt gctcgacctg gcgcgcgcca
     1741 atctttccgg cgcagtgcgc ggcacgctcg atacggcctc ggcaatcctc gaccaggact
     1801 accgcggcaa catcaacatc ctcccggaag tgtcgttgtg gcgttacatc aacgtcacgc
     1861 gcaatcccac gctggaatcg gtgcagcgct tcatgctgga aggcgagcgc agcacctggc
     1921 cgcgcatgga gatgatccgc aaccagacgc tgatcagcca gacgctcgat cgctgcgtgg
     1981 accggcttga gcacacgtcg cataacatga atgtctgtga cctgccgaag aagcccgggc
     2041 tgcgcgtggt gcgcaaccgc agctgaaatg ccattttggg gtctcaccgc aagggcattt
     2101 ttggtgttat aaaatgtttg tcgtttttaa tatcgacaaa taaaatataa caagcaaaaa
     2161 ggccgtgcgg tcacctcgaa aacggcgcag agaacgcctg cgtcacttcc gcgtcgcctt
     2221 gctacagctt cgtcggcttg aacagctcgc tgggattgaa gctgatgccg atgttgccgc
     2281 gcacgtcctt ctcgccggtg accaggtcgg tgtgattggc ccaggtgacc gacagcggaa
     2341 tggtgacggc atcgctcacc gggatttcca attgcgcgtt gagcttggcg atggtgccgt
     2401 gcaccggcgc gtggtcccag tatttttcca ggcgcgcgga cagcgacagc ttcatgcctt
     2461 ccttcgacag cgcgttcttc cgcatcacca gctgcgagaa ctgcaatgcg ccgatcgccg
     2521 aacggttcag cggcccagcc ggcccatcct gcacttgccg gtagccggca ttgagcgtga
     2581 gcccgcgatc cgggccgccc cagccgccgc ggataccggc gccatactca tcgttgccga
     2641 attgcggctt gcgcgacacg ccgttgatgt acagcgtcca cagcgtgccc tggtcgatct
     2701 cctgcaggat ttcgtttgcc gcgctgtcca ccatgtcggc ggcagcggcc acgctgcggt
     2761 atttctcgac cagcgccgca tcgctggcca gcttgagacg ttcttcggcg gtgacgcaac
     2821 tcgagtgcat gtcgtggccc atggccttga gcgacttgct ggtctgcagc atggtgggcg
     2881 cgtaaagctg gtcgagctgt gcattgccct gcgcgctgcg ggcgaaaaag cgctcgaagt
     2941 tctgcggatc gcgccggtcg cgcgacccgt agaagcgata ggtgaattcc cacgagacga
     3001 tatcgctgct gttggccgcg gtttcagcgg catcgggttt gccatcgccg tcgcggtcga
     3061 acgactggcc cttgccgccg aagctgaccg agccgccgaa gcgccgcagc gtctggtact
     3121 gctcgtagcg gtactggtca tcgagtgctt ttggatcgag aaacgcttcg atggcgaaca
     3181 ggttgaggtt gaacgtggtt gcgccggcgt tctccgaggc aaaaccgttt tccaccgcaa
     3241 aagccagcag cgagggaaag tccgcgccat cggtgatgtg cgccgacgcg gccgaacccg
     3301 cggcggcgcc gctcttggtt tccgttggcg tcgagcgcgg cgcactggcg gtgagcgtgg
     3361 cgccggcgat gccggtggcg atggcgacgg gcagctggtc gaccggcgtc tcgccctcct
     3421 tgcagctgat gtccgcctgc gcgctgcctg cggcgatgaa cagcgccaga cccacggcgc
     3481 gcttcatgtg acctccagca tcaccgcgcc gcgcttgttc agcgcgtcgc tgccggctga
     3541 tgcgttcgac ttgcgcagca ggtattgcac ggcggtattg accaggagtt ccaccttgtt
     3601 ggcccaggct gcgtcggcgg tcaccgtgct ccaggaaagt tcatacgttc caggccccgg
     3661 aaagatcacg tcgtgcgtca gcacatcgcc gtttttcttc tgcacattct gcagcagcgg
     3721 ttgcaggtgg cccggatcgg cagcggacgt gagatacagc gagatgcttg cgttgggcat
     3781 gcacacggaa tatttccagg tgcaggtatc ggtgcttttg caatccactt cgagtggtgt
     3841 caggtcctgc ggtccatcgc tgcaaattgt catcgcttcc ccccatggcg gtttttcggc
     3901 aaaggctcgc ggagtccggc gccatcgtca ctttcgcccc cgcgaagcag cgccgcgact
     3961 gagcttagcc ggtgtgctgc gaaaaaagca cccgcggaat tcagcagggc cggcgcggct
     4021 cagcggaaat cgcgcgacgc cgccggcagc gcgccaagcc aggcgctgta gtcctccacg
     4081 cgctgcgcca gcacgttgat cacgccctgc tcgtgctgca gttcgccggt gaccagcatc
     4141 aggcgcgcgc cgagcaccgg ccggcgctgc gcgcggtgca cgctgttcca cagcaccagg
     4201 ttggcgatgc cggcctcgtc ttccaggctg acgaacatga tgccggtggc ggtctccggc
     4261 atctgccggt gggtgacgat gccggccacg cgcacggtga tgccgctgct gagatcacgc
     4321 aggcgatccg agcggataac ttccagccga tccagccgcg ggcgcaggat ttccagcgga
     4381 tggcgccgca gcgtgagccc ggtggcgcgg taatcggcca gcacgtcctg gccttcgctg
     4441 ggtgcgggca gcacggccgg catttcgcgc gcggcatggc cggccagaaa tcccggcagg
     4501 ttttccacgc ccagcgcgcc ccattgcgcg cggtgccgat gaccggcgag cgagcgcagc
     4561 gcatccgcac gcgccagcgc atgcagcgcc cgcgtgccga tctgcgagcg gtgcttgagg
     4621 tcgtcgatgc cggagtaggg cgcccgcgcg cgcgcctgca ccagcttgcg cgcttcgcct
     4681 tcgctgatgc cgttgaccac gcgcaggccc aggcgcacat ccggttgttc gccgctgccc
     4741 ggctgcaggg tgcaatccca gtcgctcagc gtcacgtcga ccggcaggaa gcgcacgccg
     4801 ttgcgccgcg catccgcgac cagctgcgcc ggcgcgtaga agcccatcgg ctgtgagttg
     4861 atcaatgcgg cgcagaagat ggcgggctca tggcatttga gccaggccga ggcatagacc
     4921 agcagcgcga agctggccga gtgcgactcg ggaaaaccat aactgccgaa gccgaggatc
     4981 tgctggtaga tgctttcggc gaattccggc tcatagccgt tcgccgccat gccggcgagc
     5041 agcctgtcgc ggaaatgctc caggccgccg cgcttctgcc aggccgccat ggagcggcgc
     5101 acctggtcgg cttcgccggc gctgaagttg gcggcgacca tggcgatctg catcacctgt
     5161 tcctggaaga tcggcacgcc gaaggtgcgg cccagcactt tttcaagttc gggcttgggg
     5221 aaaaacaccg gctcgatgcc tgcgcgacgc ttcaggtacg gatggaccat gccgccctgg
     5281 atcggtccgg gccgcacgat ggccacctgc accaccaggt cgtagtaagt gcgcggctgc
     5341 agccgcggca gcatggacat ctgcgcgcgc gactccacct ggaacacgcc caccgattcg
     5401 ccgcggcaca gcatgtcgta ggttttttca tcctcgtccg ggatgtcggt cagcgcaaca
     5461 acactgccgc gctgctggcc gaccatttcc agcatgcggc gcagggccga cagcatgccc
     5521 agtgcaaggc aatcgacctt gagcatcttc agcgattcga gatcgtcctt gtcccactgg
     5581 atgatggtgc ggtcgggcat ggccgcgttc tccaccggca ccagcgcatc gagcgcatgc
     5641 tcggagatga cgaagccgcc gacgtgctgc gacaggtggc gcggaaaacc gagcaggtcc
     5701 atcaccagcg tcatcagccg gcgcacgatg ggcgagtgag gatcgaagcc cagcgcccgc
     5761 agctgcggct cgatggcgtc ggcgccgtcg cgccagaaca gcgatttcgc cagctggtcg
     5821 atctggtcga gcgagaatcc cagtgccttg ccgacgtcgc ggatggcgct gcgcgggcga
     5881 tagcggatca ccgtggcggc cagcgcagca cgatcgcgcc cgtatttttc gtagatgtac
     5941 tgcatgactt cttcgcggcg ctggtgctcg aagtcgatgt cgatatccgg cggctcggcg
     6001 cgctccctgg aaatgaatcg ctcgaacagc atgcccaggc gcttgggatc caccgcggtc
     6061 acgcccagcg catagcacac caccgaattg gcggaactgc cgcggccctg gcacaggatg
     6121 ttgcgcgacc tggcccagcg cacgatgtcc tccaccgtga ggaaaaacga ttcgtattcc
     6181 agttcggcga tcagcttcag ttccttttcg atcaggccga gctcacgccc ggggattccc
     6241 gccggccagc gccgccgcgc gccttcatag gtcagccggc gcagatgctc cgtggcgctg
     6301 aggccctccg gcaccagttc atgcgggtac tggtagcgca gcaggtcgag cgagaacgtg
     6361 cagcgctgcg cgatgagcac cgtttcctcg agcatctccg gcggaaaaag tttctgcagc
     6421 gtggacagcg ggcgcaggtg gcgctcgcca ttggccagcc gccttttccc gagcgcggca
     6481 acagtgcatt tctcgcgcgt ggcggtaagc acatcgtgca gcgggcggcg cgcgcgcacg
     6541 tggtagcagg cgctgccgag cgccaccgcg cgcagcccgt gcgcgcgggc gagcctgcgt
     6601 gcgtgctcca cgcgctcgtc actgtcctgc gcaaaggtat gggccacgcc gatccagcgg
     6661 ccggccaaat cggcgccatg cagccattgc acctgtgcat cgtcggtcgc ggcgccaagc
     6721 accaggatgc ccaggcagcg gtcgaggccg cgcacaaaat cctcgcgagc gagttggtag
     6781 tgacctttgc ccgccgcgcg ccggccgcgc gtgatcagcc ggcagatctg cgtataggcg
     6841 tcgtgatcgg gcgccagcag caccaggtgc aggccgcacg ccaggcggaa ttcgctgccg
     6901 acgagcagct ggatgccggc tgtcttcgcc gcatcgtgcg cgcgcaccat gccggccatc
     6961 gagcactcat cggtgatggc gatggccgcg tagcccagcg ccctggcgcg cgccaccagt
     7021 tcgtccgcgt gcgaggcgcc gcgcaggaag ctgaagttgg agaggcagtg aagctcggcg
     7081 tattgcatgc gcaacgggaa agccggcccg gcctgagcca ggcccgggct tgccgggaat
     7141 caggcccaca cgccatgcag gtaccagcgc aggttgtggc cgtcgcggta cagccacagg
     7201 cggctgccgt cacgggtctg cgcggtgtag tagtcacggc ggctgtcgtc gccttcccac
     7261 cagccatcct cgatgcgctc ggggcgcgag gtgagcagcg gcagcacgtc gacctgcttg
     7321 ggttgcggca gcagccacag cgggcgctcg ggcagggggc cgggaatttc cggcgccggg
     7381 ccgtcgcgca gcacgcacca ggctttctcc gggcggtgat cggattgcag gcccagcgtg
     7441 cgcaccgcct cgcggcccag gcgcgcggac agcttgtcga gcaggccgaa ccattcctcg
     7501 tcctggcgct tgtggccgtc gaagaaatcc agctgggtgt cgccgggatc ggcaaagctg
     7561 ctggcctgca gccgcaggcg gatcaccggc gcagcgatgg ggctgcggtt cagctgctcg
     7621 cgcagaagag agtgcagctg cgcggcatcg cgctgcgggc ggctggtctt cacctgcagc
     7681 gtggtgggcg gcacgtgctc atgctccagc accagttcga actgctgcac gccggtgtcg
     7741 cgcccgcgca ggtagccctg gaattcgccg agcatgcggc gcagcgagaa gcccaggccc
     7801 tcggtggttt cgatggcggc attgaattcg atctgccgct ggtaggtttc cggcggacgg
     7861 taagggcgca tggtctccgg ccgcatgccg atcaggcggc gcaggtattc ggtggtgtcg
     7921 cggctgaagc ggcgcgccag ttcgtcgtaa cccagcgcgt agatctcgcc gatgcggcgc
     7981 aggccgctgg cctgcaggcc ctgcagcaca tcatcgggca gggccagtgc actgatcgga
     8041 aatttctcga tgcgcgcggt gatctgcttc ttctcggtga ccggcggctg cggcgccagc
     8101 agcgcgcaca gcgcggcggc ttccagcgtg ggtgccacgc caatctgcgc gcggtaaccc
     8161 agcgcttcga tgccggatgc gatcagctgc tgcagccgcg ccagcccgcc gaacagcttc
     8221 aggctggtgc caacctcgat ccacagcagg cgccgcgtgg tgtgcgcgct gatggtgctg
     8281 ctgtactggt aggcccagcc ggccagcgca cgcagggttt cacgctcgcg ccgcggcgaa
     8341 cgctccagca gttttatctg cggcgccagc gcctgcgccg aggcggcgtc gaggcccacg
     8401 gtaatgccag cctcggtcgc ggccaccgtg cacgacagca catggcggcg gctgccttca
     8461 tgctcgatgg ccgcggcgag gttttcactg gaagcgagtg cttccagcgg caattctggg
     8521 agatggatgc agagccagag catgatgcgc tcctaatgga tgttgctttt cccctctccc
     8581 accgggagag gggcaggggt gagggcagtc ggtcggatgc aactcgggtg ctggctatgc
     8641 gtgcgtccct cacccgcgcc tgcggcgcga cctctccccg ggggagaggc aagaagcgca
     8701 attgtgggcg aagaacttga agcagtttca acgtggaaca gactcgcccg ttctcgcagc
     8761 gggcaaagca catcggcgcc ctggcggccg cccttggcct tctgcacgtc cacgcgcagg
     8821 ccaccgacgc cggggcgcag cgccagccgc acgctggcga tcgatgcctg cttcagcgcc
     8881 ggcgccggcc ggtagcagaa gcacagcgcg ctgccgttct cggcgcccag ctggatgcgg
     8941 cgcagctcgg tatccgaaag gcggcgcgtc cacagcagca ccgcgcccac cgcgccgctc
     9001 ttgagcatct gcaccgcgct ccagccggca tcgtcgggcg ccagctcggg cagccacagc
     9061 acgcgttcgg gcgcgatgcc ggcgcgcacc agcgcgggcg cgtagggaat gtagggcggg
     9121 gcgatgaagg cgatgcgctt gccgcgcgcg gacagccgcg ccagcgcggg aagtgtcagt
     9181 gatagttcgc cgatgccctc aacctgcggg atcagttcgg tgagtgcgcc cagcggccag
     9241 ccatggcctg gcaatacttg atccagcgcg gcaaaacccg tcggctcggc cgcgatcttg
     9301 gcgatggagt gaccggtcca gaggcggcga tctcccagtt cgcggacgat ggcttgcgcg
     9361 cggctcatgt gcaggcccgg gcatcaggcg cgatggatgt ggccgcaata cagcccttcg
     9421 atctcgaact cttcctgcgt gaggtcgatg tcgatgggcg ccagcgcggc attgcgcggc
     9481 agcagctgca catggtgccc gcgccggcgg tatttcttta cggtgatctc cggctcgccg
     9541 tcgcggaaca gcttggccac gatgacctgg ccgctgtcgg cgtcgggctg cgcgtgcacg
     9601 gccagcaggt caccgtcctg gatgcccatc tcgatcatgc tgtcgccatg aaccttgcgc
     9661 aggtaatccg ggcgcgggtg gaacagggca gggtcgatgc gcagccagtc gtccacttca
     9721 tcggtggaca gcaccggcgg gccggcggcc acgcggccca ccagcggcag cagcgtggga
     9781 tcgcgcatcg gcttcaccgg cggcaggcgc acgccgcgcg cgcggccggg atgcagcgtc
     9841 agctcgccct tcttcgccag gatatgcagc cagtcatgcg cagccttgcg cgtgaccttg
     9901 aacgcctccg caatgtcggc gcgcgagggc gccatgccgt gctgcacggc gaactggcgg
     9961 atgaaacgga gcacttcggc ctggcgggga gtgagttcgt ccatgccgct tttatactac
    10021 tcatccggta agtaatcaaa tgattagtat cacgggcgcg aaatgccccg ccatggcctc
    10081 accgcaccgg gcaaaatcat gttaataatc atcatgcaaa ataacattca tcagatgatt
    10141 aataacagtt gaaatgccgg tgcggtggct ctttttcggc gccccggagg gcatccactg
    10201 agggtttccc ccggcccatt tgtcagcagc ggctgacagc gcgctcagcg ccgctccggc
    10261 tgtaatcggg cggcgaaaac gcagaatgac cagcatcaga tttccacata gaaaagccat
    10321 gtcctcgacc accacccaga atgccgttcc caccatcgtt cccacgcgtc cttcgaccca
    10381 gtggccggtg ggcgtgcgcc tgcgcttcaa gcccgatgcc gagctgcacg cgcagcacgc
    10441 cacgctcaag ggcacgcagg tgctggtact gtcgggcctc aagctgatcg gcccttcagg
    10501 tccggacctg cgctgctcct ggcgccagga aatcctctcg tttgccgccg gctgccgcgt
    10561 gggctgggcc cgcccggacc agctggaact gccgctggac gaggaagaag acgccatctg
    10621 agcgacttcg gcatccgctg aatccaagcc cgcgcaagcg ggctttttca tgtgcgcggc
    10681 gcgcattgat cggcgtcaat gcgccgtccg gccgatggct cagcatgcgc gcatggacac
    10741 tgccgcattc gacgccgacc tggtccgccg ctacgaccgg ccgggcccgc gctacacctc
    10801 gtaccccacc gcgctgcagt tccgcgagga tttcggcccg gagcaatacc gcgaggccat
    10861 tgcgcgcagc gagattgccg gcgggccgct gtcggtctac gtacacgtgc cgttctgcgc
    10921 caacccgtgc tactactgcg gctgcacacg catcatcacg cgccagccgg ccgtggtgag
    10981 caagtacctg gcgcgcatca tcgacgagat ccgcctgcag tcgctgcgct tcggcccgca
    11041 gcgcccggta gagcagctgc acctgggtgg cggcacgccc acttattttt ccgccgagca
    11101 gctggcggaa ttgatggaag cgctggccgc caacttcaac ctgcgccacg atgcccgccg
    11161 cgagttttcc attgagatgg atccgcgcac ggcgcagccc ggactgatcc aggcgctggc
    11221 cgccatgggc ttcaaccgcc tgtcgctggg cgtgcaggat ttcagccccg acgtgcagcg
    11281 cgcggtgaac cgcgtgcaga cggtggacga caccgccgcg ctcatcgccg aagcgcgcaa
    11341 ggccggcttc acctcggtgt cggtggacct gatctacggc ctgccgctgc agacgctgca
    11401 atcgttcggc cgcacgctgg cacaggtggc atcgctgcgg cccgaccgca tcgcggccta
    11461 cagctacgcg catctgccgg aacgcttcaa ggcgcagcgc cagatcaaca gcgccgatct
    11521 gcccgacgcc gagaccaagc tcgcgctgct cggcctcacc gtgaacgtgc tgaccatggc
    11581 cggctaccag tacatcggca tggatcactt tgccttgccc gaagacgaac tggcggtggc
    11641 gcagcgcaac ggcacgctgc atcgcagctt ccagggttat tccacgcacg gcgggctgga
    11701 cctgatcggc ctgggcctgt catcgatcgg ccgcgtcgcc gacacgtact cgcagaactt
    11761 caagactttg gaccgctact acggtgcgct cgacgcgtgg gagctgccag tcgagcgcgg
    11821 cctgcgcctg tcagccgacg accgcgcgcg gcgcagcgtg atcgaatcga tcatgtgtca
    11881 ggggcgggtg gattttcacg ccatcgagcg cgagcaccgc atcgaattca acagctactt
    11941 ccgagaaagc ctgcgccagc tggagccgtt gcagcaggat gggctggtgc gcctttcggc
    12001 gcatcacctg gaagtgacgc cgcgcggctg cctgctgctg cgggcggtgg caatgccgtt
    12061 tgatgcctat gcggcgccgg gggtggtgca gcagcggtac tcgaagatga tttagctctt
    12121 cgtgctcatt ttattattta acataatata caatgtaccc gtagacggaa accccgaaaa
    12181 agaggttttc cgaggcgcgc gatgtgccga ggtggttccg tcaaagcacg ttgggggcgc
    12241 gccaagataa agcggaaatg tgcccacatc gcctttaaag tgctgtatca aatgcgtttt
    12301 atttaatacg catttgtatc aaggcgtcag gatcactgaa aattccatcc acaccgctgg
    12361 acgagtttgc tgatcttcgc cttcgcttac ttctcgagca agccctgctg gacagttttc
    12421 gacgaaagct tcttggcgtg caacctctcc aattgcgctt gcagcgcctc cgttcgaccc
    12481 tgctgaactg cggcgtcacg cattgccgcg tctctctctc gtcgcaattg ctctacgagc
    12541 agacgctcag catcgcgttc tgccaaggcg gccgcgcgct ggccgttggc ggcgtcgatc
    12601 tctcgggacg ccgcctccac ctttgttttg aggtccgatt cggctgtagc gcggctccgc
    12661 tgctcacgcg acagatctgc attgaggcgg atcacttcct tgcgctgctc ggcgatctcg
    12721 gtgaccagtt ctgcgccctt gctctcaagg tccgcaatcc gcctggcggc tgttgcctgc
    12781 tccttttccc gatcaccacc cagcttcttg agctgggtct tgaggtcgtc gatctgacct
    12841 aagaaatgcc gctcgttgcc ctcggcgcga tccttttgcg aggcaatttc ctcttggtgc
    12901 ttgcgcgacg cgcgctcgcg ctcatccgtt gcagcctggg cttttgcctc cgccgcgctg
    12961 gcattgcgcg cctggcttgc cgcctcggcc tgcgcagcga ccagctgtcg ctccaaggca
    13021 gtgtggcgct ctttcagccc ggctaattcg gtctccagtg ccgtcgttgc gctgcgcaac
    13081 tgcgcgcgtt ctgtctcgaa ggcattgcgc tcggcctgga cctggtcacg ctccagtttg
    13141 agcgcagcct gctgctccgc atcacgcttg tcgacgtctt cctgagccgc atcacgcacc
    13201 ttcttgtaga gctcggcggc ggcaatggcg atgggcgccg gcaggttgag gcggtcaggc
    13261 aggctcttga aatactgctt gagcagcgga ttgaccgtgt tcggcgatcc ggtccctagg
    13321 tgcgcgcgca cgctatcgat cgtgggcctt tcgcccttgg cgaggacggc gtcggcggcc
    13381 cgggtgacgt cttcgatggt gacccctgct ggcattttcc tgctccggtg cgatgaccct
    13441 attctaaatt tggcggtttt gagttatacc tacgacaagg gttccttatc gtaggtataa
    13501 ctctttaact gtattaaata taacatacta tgtatgaata acaacataaa acacaccgct
    13561 gaaacactgc tagcgaaggc tgcagagggc tctgcagcgc tgccggccct tcctgaatcg
    13621 cggggcgtcg tgccggccaa cctcgaggct caagtcgagg ctgcggtgga tgcctttcga
    13681 gcggcagcca catcgccggc gacctgggag tcgtactccg gggcattgcg ctacgtctgc
    13741 gcctgggcgg ccctgcggtt cggcaagcct ctggagttcc ccgttcccgc gtcggtcgcc
    13801 aagctatttg taattgacca tttcgggcag ccagaacgca tcaacgccgg cagcggccgc
    13861 accaagctga tgctggagct gaccctccct gccgccgttg accaggctct cgttgatggc
    13921 ggattcaaag ccaagctcgg ccgattgaaa atgagcacga tcgatcatcg tctgtgggtc
    13981 ct
//
LOCUS       DASZZC010000780         6166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_124783,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000780 DASZZC010000000
VERSION     DASZZC010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124783"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VHE37_16855"
     CDS             complement(<1..1041)
                     /locus_tag="VHE37_16855"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT37257.1"
                     /translation="MPLLCALRVTAAWAQDSTPTPGVPTIPVQSQAAAEPEPAEAGPR
                     AAQLDEVVVTATKREANPRDLPASVSAVRGEDLEKHGYQGQEDFLKLIPGVTFANDTV
                     TANRITIRGIGADLNTSNTTGVFIGDVPFEDPTLPRVTLDPNPFDLARVEVLKGPQGT
                     LFGGSALNGAVRYVPQDPELGSWEARTFAQYEVVNQGGMAPVAGAAVNVPAGDTLAFR
                     LVGFHRNAAGWVDDLQRHLQDVNTLVQDGGRLMALWQPDGRWKVSAMGVVQDTRVADS
                     SITDNRDGRLSRSDTPQASPINTRYDLETLGVQYSFPAFDILSQTSRTSKHFGGIEDA
                     SRIGNLTDNPPPS"
     gene            complement(1119..2699)
                     /locus_tag="VHE37_16860"
     CDS             complement(1119..2699)
                     /locus_tag="VHE37_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVT37258.1"
                     /translation="MEAGSPTRPWIVPLREAAAARLEGIERFELGLQGRALAACAGLP
                     SGYAKGRRRHYPLLITLNLVELFGAAVEMSRLLAGSREVGRCVVVNVGGIRAGDLPQL
                     AEVVARCRQRYRIDAREIAVVADGTMAHDLLAAFRRGIAGVTRCMLASARATMDAPPV
                     PGAPLAWTVMEPLTGAELARHDVRWHVAAEASSASLALPALLHGLRTFWSTGHDYGIE
                     IMPLASPAVARIACALAPVLRPLTRRRRFAPQSGSRHVVRSRVMQRDFEIFIALPPPA
                     EEDPGRRYPAIVALDANGGFATVSEIAARLARTGEIEPALVIGIGVPRAQGELEYGFR
                     RFEEFSPPLPPQDDAGDGLARFFRALFALRGEDARVQLGQAPGFHRFIVDELLPQLVP
                     QLPLDTDALMLLGHSAGGTFVGYALAQADSPFTRYACLSPGVNVSGQWMLRRAAAPLP
                     PQSSAARVYGSIGSAELDNRFNRLAGIPMTRAYIGALAEQGAQHTQFHVLDGETHTSV
                     YVRAVAHALRMLLPRRAP"
     gene            2926..3519
                     /locus_tag="VHE37_16865"
     CDS             2926..3519
                     /locus_tag="VHE37_16865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT37259.1"
                     /translation="MTREQKKAQRAQALLDAAWAAFCELGYEKLTIEDVADRAGYSRQ
                     PVYTLFGDKQNLIFELQSRATRQVVDLLFAHLRPGASLRENLTRVAQVVASELGSNHP
                     TYGEQLFIVAQAIALNRPDIADKVRAQARWVIDDIARVIQRSPLARGEKLRSQPETIA
                     AHLAAHINGLTTVQYQTGKRYASARELTEIFLFFALE"
     gene            complement(3921..5900)
                     /locus_tag="VHE37_16870"
     CDS             complement(3921..5900)
                     /locus_tag="VHE37_16870"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HVT37260.1"
                     /translation="MGNVYFNTLVDLDGHGLLALTTDTSNSYSAPLPAQKYGYAGWLA
                     PGAGELQDRLELCTRPRSEEQMLDRVDDLHRKMAIRQDAQAELKWLRTQVDESFREVP
                     TAAHPMGNGLVFLMFVWELEDSQIADTELRVIDVPNNRLLYSTTPEALSEELGRRKPG
                     DRAAEGSWATHPLVQDSGRGYVQITFDDEMLLRWDGDRLRYVSSAIVRQHRIAIGAEH
                     VFCHHWNREPLAVLPLADPTNVREVPLRHGRKGWWEHRAALAADRCVIAHAGGTVEII
                     DGHGNEVLAVRPDPSMGRKDQMQASITPDGRHVLTRRWDGTSAIDVERGMVAEVLMLS
                     DVRYLGQDDPPPNRLVYRECCLATNEGVWQQPWSIAIASRPESLMPQLIRWEELDWQP
                     GLRPGSGKGKKKGPAAYKTLLPYWRKPSLALTPAKSGKLRTRLYGAPDLPEGFAWPEH
                     EGRRMLLLCQVDLAEVGEKLGAPWPQQGGLLLFVAVDEDNDNELLIDDSFNPVATRLL
                     HVPAFGPTAPGGPQFPAQKVRLRKDKTELPQADAAIVLRELLSDEDREQYRTYAEERQ
                     DEGPTGGHRLGGYPANVQGNQLEAAAEEIINEDAPEEDAEGWGRAARWRLLLQLDSDD
                     HIMWGTDMGTLHVMIHDDDLAAGEFGRAAVIVEGH"
BASE COUNT         1017 a         2092 c         1999 g         1058 t
ORIGIN      
        1 cgacggcggc gggttgtcgg tgaggttgcc gatgcgcgag gcgtcctcga tgccgccgaa
       61 gtgtttcgac gtgcggctgg tctgcgacag gatgtcgaac gccgggaacg agtactgcac
      121 gccgagggtt tccaggtcat agcgagtgtt gatcgggctg gcctgcggcg tgtctgagcg
      181 gctcagtcgt ccgtcgcggt tgtcggtgat ggaagaatcg gccacgcgcg tgtcctgcac
      241 cacgcccatc gcggaaacct tccagcggcc gtccggctgc cacagcgcca tcaggcggcc
      301 gccgtcctgc accagcgtgt tcacgtcctg caggtggcgc tgcaggtcat cgacccagcc
      361 ggcggcgttg cgatggaatc ccaccaggcg gaaggccagc gtgtcgccgg ccggcacgtt
      421 gaccgcggcg ccggccaccg gcgccatgcc gccctggttc accacttcgt actgcgcaaa
      481 ggtcctggct tcccagctgc ccagctccgg gtcctgcggc acgtagcgca ccgcgccgtt
      541 gagcgccgag ccgccgaaca gcgtgccctg cgggcccttc agcacctcca cgcgcgccag
      601 gtcgaacggg ttgggatcga gcgtgacgcg cggcagcgtc gggtcttcaa acggcacgtc
      661 gccgatgaac acgccggtgg tgttgctggt gttgaggtcg gcaccgatgc cgcggatggt
      721 gatgcggttg gccgtgacgg tgtcgttggc gaaggtcacg cccggaatca gcttgaggaa
      781 atcctcctgg ccctggtagc cgtgcttctc caggtcttcg ccgcgcaccg ccgacacgct
      841 ggcaggcagg tcgcgcggat tggcctcgcg cttggtcgcc gtcaccacca cttcgtcgag
      901 ctgcgccgca cgtgggccgg cctcggcggg ctccggctcg gccgccgcct gcgactgcac
      961 ggggatggtc ggcaccccgg gcgtcggcgt gctgtcctgc gcccatgccg ccgtcacgcg
     1021 cagtgcgcag agcagcggca cgtatctcca tcgcatcggc tgcaacatcc tcctgcctcc
     1081 agcggcgcat cgcgcaccgc gctgtcgaat cgcgttgctc acggcgcgcg ccgcggcagc
     1141 agcatgcgca gcgcatgcgc gacggcgcgc acgtaaacgc tggtgtgcgt ttcgccgtcg
     1201 agcacgtgga actgcgtgtg ctgcgcgccc tgctccgcca gcgcgccgat gtaggcgcgc
     1261 gtcatcggta tgccggccag ccggttgaaa cggttgtcca gttccgcgct gccgatgctg
     1321 ccgtagacgc gcgccgcgct ggactgcggt ggcagcggcg cggccgcgcg gcgcaacatc
     1381 cattgaccgc tgacgttcac gcccgggctc aggcaggcgt agcgtgtgaa cggcgagtcg
     1441 gcctgcgcca gcgcgtaacc gacaaaggtg ccgccggccg aatgtcccag cagcatcagc
     1501 gcatcggtgt ccagcggcag ctgcggcacc agctgcggca gcagctcatc gacgatgaag
     1561 cgatggaatc cgggggcctg ccccagctgg acgcgcgcat cctcgccgcg caatgcaaac
     1621 aacgcgcgga agaaacgggc cagaccatca ccggcatcat cctgcggcgg cagcggcggc
     1681 gagaactcct cgaagcgccg gaagccgtat tcgagctcgc cctgcgcgcg cggcacgccg
     1741 atgccaatga ccagcgccgg ctcgatctcg cccgtgcgcg ccagccgcgc cgcgatctcg
     1801 gaaaccgtgg caaagccgcc gttggcatcg agcgcgacga tggcaggata gcgacggccc
     1861 gggtcttcct ctgccggcgg cggcaaggcg atgaagattt cgaagtcccg ctgcatcacc
     1921 cgcgagcgca ccacgtggcg cgatccgctc tgcggcgcaa accgccggcg ccgcgtgagc
     1981 gggcgcagca ccggcgccag tgcgcacgcg atgcgcgcga ccgcgggcga tgccagcggc
     2041 atgatctcga taccgtagtc gtggccggtg ctccagaacg tgcgcaggcc gtgcagcagt
     2101 gcgggcagcg ccaggctggc cgaagaagct tcggctgcga catgccagcg cacatcgtgg
     2161 cgcgcgagtt cggcgccggt aagcggttcc atgaccgtcc aggcaagcgg cgctcccggc
     2221 accggaggcg cgtccatcgt tgcgcgcgca ctggccagca tgcagcgagt cacgccggcg
     2281 atgccgcgcc ggaaggccgc cagcagatcg tgggccatgg tgccgtcggc gaccaccgcg
     2341 atttcacgcg catcgatgcg gtagcgctgg cggcaacggg ccacgacttc ggccagttgc
     2401 ggcaggtcgc cggcacggat gccgccgaca ttgaccacca cgcagcggcc gacctcgcgc
     2461 gagccggcca gcagccggct catctcgacc gcggcgccaa aaagttcaac caggttcagg
     2521 gtgatgagga gcggatagtg ccggcgccgc cccttggcat agcccgacgg cagcccggcg
     2581 caggccgcca gcgcgcggcc ctgcaggccc agttcgaacc gttcgatgcc ttcgagccgc
     2641 gcggccgcgg cctcgcgcag cggcactatc cacggcctgg tcggactgcc cgcttccact
     2701 gtgctcctcg ctcgccgtgc cggagacggg aacgctccag cgacaaacta ttcattgtgt
     2761 ctagaaagtt atcatgttta gtttgtctgg cgcaacggcg atgcatggac aatttacgac
     2821 gagcattcgc agacaaacta tgccgcgatg tatagtttgt tcatggcacg gccaaagacc
     2881 cttccggcga gttcgcccgc gacgacgccg cgggtacgca cgcgcatgac gcgggagcag
     2941 aaaaaggcgc agcgcgcgca ggccctgctc gatgccgcct gggcggcatt ctgcgaactg
     3001 ggctacgaaa agctgaccat cgaggacgtc gccgaccgcg ccggctattc acgccagccg
     3061 gtctataccc tgttcggcga caagcagaac ctgatcttcg aactgcagag ccgcgcaacg
     3121 cgccaggtgg tggacctgct gttcgcgcac ctgcgtcccg gcgcgagcct gcgcgagaac
     3181 ctgacgcgcg tggcccaggt ggtggccagt gaactgggca gcaaccaccc gacctacggc
     3241 gagcagttgt tcatcgtggc ccaggccatc gcgctcaacc gtccggacat cgccgacaag
     3301 gtccgcgcgc aggcgcgctg ggtgatcgac gacatcgcgc gcgtgatcca gcgctcgccg
     3361 ctggcgcgcg gcgagaagct gcgcagccag ccggaaacca tcgccgcgca cctggcggcg
     3421 cacatcaacg gactgaccac ggtgcagtac cagaccggca agcgctacgc gtcggcgcgc
     3481 gaactcaccg agatcttcct gttttttgcg cttgagtagc gcagcgcctc cggcattgcc
     3541 tggcactgtc ctgtagctga cctccctgca gtgactggcc ggcgccgaat gtgcccggaa
     3601 gtaaggtttg tccgaaacgg acgaaccttg atggaaacat ttttatccat tttcccccgg
     3661 gaaacgtgat gcatgtagag acttttatgg attgagccca accggcgata ccgcgaatca
     3721 atacttctcg ctgctcatca tggctgtgag atgacgaagc tcgcgtgatt tactgatcgt
     3781 ggagggcatt gaattcgtga ggtgcctgcg ggccaggagc ggaagtttag gaacgtctgc
     3841 tttggcggaa ctcgaactgg gtgaacggca cgttacgcga tgaactcgct gaacctgccc
     3901 ctacgctcac gggcctaccc tcaatgcccc tccacaataa ccgccgcccg cccaaactca
     3961 ccggcggcaa ggtcgtcgtc gtggatcatc acgtgcaggg tgcccatgtc ggtgccccac
     4021 atgatgtggt cgtcgctgtc gagttgcagc agcagacgcc agcgcgcggc gcggccccag
     4081 ccctcggcgt cttcttccgg cgcgtcttcg ttgatgattt cttccgcggc ggcttccagc
     4141 tggttgccct gtacgttggc gggatagccg ccgaggcggt ggccgccggt ggggccttcg
     4201 tcctggcgct cttcggcgta agtgcggtac tgctcgcggt cttcgtcgga gagcagctcg
     4261 cgcagtacga tggccgcgtc cgcctgcggc agctccgtct tgtccttgcg caggcgtacc
     4321 ttctgcgcgg ggaactgcgg gccacccggc gcggtggggc caaaagcagg tacgtgcagc
     4381 agccgtgtcg cgaccgggtt gaagctgtcg tctatgagca gctcgttatc gttgtcttca
     4441 tccaccgcca caaacagcag caggccgccc tgctgcggcc acggagcgcc gagcttttca
     4501 ccgacctcgg cgaggtcgac ctggcacaac agcagcatcc tgcggccctc gtgttcgggc
     4561 catgcgaagc cctccggcag atccggcgcg ccatagagcc gcgtgcgcaa cttaccggac
     4621 ttcgccggcg taagcgccag cgagggtttg cgccagtacg gcagcagtgt cttgtacgcg
     4681 gcgggtcctt tcttcttccc cttgccgctg cccgggcgca ggccgggttg ccagtctagt
     4741 tcttcccagc ggattagctg gggcatcagt gactctgggc ggctagcgat ggcgatgctc
     4801 cacggctgtt gccacacgcc ttcattggtg gcgaggcagc actcgcggta gacgaggcgg
     4861 ttcggcggcg gatcatcttg gccgaggtat ctcacatcgc taagcatcag cacctccgcc
     4921 accatcccgc gctcgacgtc gatggccgag gtgccgtccc agcgtcgggt cagcacatgg
     4981 cgtccgtccg gcgtgatcga agcctgcatc tggtctttgc ggcccatgct ggggtcgggg
     5041 cgaacggcca gcacttcgtt gccgtggccg tcgatgatct caacagttcc gccggcatgt
     5101 gcgatcacgc agcggtccgc agccaacgct gcgcgatgct cccaccaacc cttgcggcca
     5161 tggcgcagcg gcacttcgcg cacattggta ggatcggcca gcggcaggac cgcaaggggc
     5221 tcgcgattcc agtggtggca gaacacgtgc tccgctccaa tcgcaatccg gtgctgccgc
     5281 actattgccg aagacacgta ccggaggcga tcaccatccc agcgcagcag catttcgtcg
     5341 tcgaacgtga tctgcacgta accgcgcccg ctgtcttgga ccagaggatg cgtcgcccaa
     5401 ctaccctccg ccgcgcggtc tcccggcttg cgccgtccca actcttccga cagcgcctcc
     5461 ggcgtcgtcg aatacagtag ccggttgttt ggtacgtcga tgacgcgcaa ttcggtatcc
     5521 gcgatctgcg aatcttctag ctcccagaca aacatcagga acaccagtcc gttgcccatc
     5581 ggatgcgcgg cagttggcac ctcacggaag ctctcgtcca cctgtgtgcg caaccacttc
     5641 aactccgcct gcgcgtcctg ccggatcgcc atcttgcggt gcaggtcatc tacacggtcc
     5701 agcatctgct cctcggagcg aggacgcgtg caaagttcca gccgatcctg cagctcaccc
     5761 gcaccgggcg ccaaccatcc tgcgtacccg tatttctgcg ccggcagcgg tgcgctatag
     5821 gagttggacg tatcggtggt cagcgccagc aacccatgtc cgtcgagatc cactagcgtg
     5881 ttgaagtaaa cgttccccat gtatttccct tcccccaaac tgagagtcgc tcacctcccc
     5941 agacgtcaac gtaatgtccg cttctcggaa gtgtgaatca acgggctacc gtccgcttcg
     6001 ggtcgctatg cagagctctg catagcgacc cttatgcgga ctacgaaact atgcggagtt
     6061 cgttgagctt cccggtcgag gcggcgtcga aatagtcgac atagtttttg ccggaaggtg
     6121 cagttgcggc cccatctcag ggagaccgca atggacagca ccacgc
//
LOCUS       DASZZC010000781         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_124814,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000781 DASZZC010000000
VERSION     DASZZC010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124814"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /locus_tag="VHE37_16875"
     CDS             <1..2235
                     /locus_tag="VHE37_16875"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT37261.1"
                     /translation="REILEQDLAGKIMAAADAEASPAVKVHVTGAAIIRAATSNSVTR
                     QLRWVVPAFVVMLAVFLGFTFRSWAGVLLPLATICIALTLTLGTLAALGHPLNLITSL
                     VPPLLVTMGLAYCAHILCEYQQLGGEGQMADAQMRIALLLSEVTGPVLLTGFTTAVGL
                     LAIAFNPLPAITEFAWLSAVGVFYTVVLVLTFLPAALRFMPAARAHDGRSERLFERVG
                     RRVGEFDVRQRRLILLCAGLLAVVALAACTLIRVGDQFVGIFPERARVRADFEAVNRL
                     LGGVNPLSIVIDGPTDIFTEPYVLRSLDELEQWLLHQPEVGAVTTLSDHVKELAANLA
                     HAQNAGIPSSRDMVKQLLFFGDGELLRGVVNSQRSSTLVTLRLRVDDTADISEFVERL
                     RPELDRLPTGLTAHITGNTMLVTDSVAGATSGQIQSVAFALFVIYLCLALQFASWSVG
                     LLATLPTALQTAIYFGALGISGITLNATTSLVECLVLGLAVDDTIHYLARFNTTARRV
                     GSEQNAAVAALSAVLRPVTLTKVILALGFLMLITGDLRNQVLFGWLAALTLATAWMVD
                     VFVTPAFISGVRVVTLWDSLRLNLGHDVQGTIPLFAGLSTRQARVFALMSQIIEVPAG
                     TRLMTEGESAGDGEKGDPRGDVYVVIDGQLQVWTERDGARRDLTTLGRGAVVGETGYF
                     GQRRMATVDTLTRARLLRFDDADQEMVMRRYPKIAARVFLNLNRIQAARRAENARVAV
                     TAHG"
BASE COUNT          339 a          829 c          837 g          427 t
ORIGIN      
        1 cgcgaaatcc ttgagcagga tctggccggc aagatcatgg ccgcggcgga tgcggaagcg
       61 tcgcccgcgg tgaaggtcca cgtgaccggc gcggccatca tccgtgccgc caccagcaac
      121 tcggtgacgc gccagctgcg ctgggtggtg ccggccttcg tggtcatgct ggcggtgttc
      181 ctcgggttca ctttccgcag ctgggccggc gtgctgctgc cgctggccac catctgcatc
      241 gcgctgacgc tgacgctcgg cacgctggcg gcgctgggcc atccgctcaa cctgattacc
      301 agcctggtgc cgccgctgct ggtgaccatg ggcctggcct attgcgcgca tatcctgtgc
      361 gagtaccagc agctgggcgg tgaagggcag atggccgacg cgcagatgcg catcgcgctg
      421 ctgctgtcgg aggtcaccgg gccggtgctg ctcaccggct tcaccaccgc ggtcggcctg
      481 ctggccatcg ccttcaatcc cctgccggcg atcaccgagt tcgcctggct gtcggccgtg
      541 ggcgtgttct acaccgtggt actggtactc accttcctgc cggcggcgct gcgcttcatg
      601 cccgccgcgc gcgcgcacga cggccgcagc gagcgcctgt tcgaacgcgt cggccgccgc
      661 gtgggcgagt tcgacgtgcg ccagcgccgc ctgatcctgc tgtgcgccgg cctgctcgcg
      721 gtggtggcgc tggcggcgtg cacgctgatc cgcgtcggcg accagttcgt cggcatcttt
      781 cccgagcgcg cgcgcgtgcg cgccgacttc gaggcggtga accgtctgct gggcggcgtc
      841 aacccgctgt caatcgtcat cgacggcccc actgacatct tcaccgagcc ctacgtgctg
      901 cgttcgctcg atgagctgga gcaatggctg ctgcaccagc ccgaggtggg cgctgtgacc
      961 acgctgtcgg atcacgtgaa ggaactggcg gccaacctgg cgcacgcgca gaacgccggc
     1021 atcccgtcgt cgcgcgacat ggtcaaacag ctgctgttct tcggcgacgg cgaactgctg
     1081 cgcggcgtgg tcaacagcca gcgttcgtcg acgctggtca cgctgcgcct gcgcgtggat
     1141 gacaccgccg acatctcgga attcgtcgag cggctgcggc ccgaactgga ccggctgccg
     1201 accggcctca ccgcgcacat caccggcaac accatgttgg tgaccgactc ggtggcgggc
     1261 gccacctccg gccagatcca gagcgtggcc tttgcactgt tcgtcattta cctgtgcctg
     1321 gcgctgcagt tcgcctcgtg gagcgtgggc ctgctggcca cgctgccgac cgcgctgcag
     1381 accgccatct atttcggcgc gctgggcatt tccggcatca cgctcaatgc cactaccagc
     1441 ctggtcgaat gcctggtgct ggggctcgcg gtggacgaca ccatccatta cctcgcgcgc
     1501 ttcaatacca ctgcgcgccg cgtcggctcg gagcagaacg ctgcggtggc ggcattgtcc
     1561 gccgtgctgc gcccggtgac gctgaccaag gtgatcctgg cattgggttt cctcatgctg
     1621 atcaccggcg acctgcgcaa ccaggtgctg ttcggctggc tcgccgcgct cacgctggcc
     1681 accgcgtgga tggtcgacgt gttcgtcact ccggccttca tcagcggcgt gcgcgtggtc
     1741 acgctgtggg acagcctgcg cctcaacctg ggccacgacg tgcagggcac catcccgctg
     1801 ttcgccggcc tgtcgacgcg gcaggcgcgc gtgttcgcgc tgatgtcgca gatcatcgag
     1861 gtgccggccg gcacgcggct gatgaccgag ggcgaatccg ccggcgacgg cgagaagggc
     1921 gatccgcgcg gcgatgtcta cgtggtgatc gacggccagc tgcaggtgtg gaccgagcgc
     1981 gacggcgcgc gccgcgacct gaccacgctg ggccgcggcg cggtggtggg cgagaccggc
     2041 tacttcggcc agcggcgcat ggccacggtg gacacgctga cccgcgcgcg cctgctgcgc
     2101 ttcgatgacg ccgaccagga aatggtcatg cggcgctacc cgaagatcgc cgcgcgcgtg
     2161 ttcctcaacc tcaatcgcat ccaggccgcg cgccgagcgg agaacgcgcg ggtggcggtg
     2221 acggcgcatg ggtgaatgcc tgggtgggtt tccgccggag taggcggagc gagttcagcg
     2281 ctgggcgcta tcgaccctta tgcggactac gaaactatgc ggagttcgtt gagcttcccg
     2341 gtcgaggcgg cgtcgaaata gtcgacatag tttttgccgg aaggtgcagt tgcggcccca
     2401 tctcagggag accgcaatgg acagcaccac gc
//
LOCUS       DASZZC010000782         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_125033,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000782 DASZZC010000000
VERSION     DASZZC010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125033"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHE37_16880"
     CDS             <1..331
                     /locus_tag="VHE37_16880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT37262.1"
                     /translation="WEQEELPDPSLDEGHALHLFRIMREAVTNALRHGQATRVRVRVK
                     RHAAMLMMDVADDGPGAPLPDADPGRGMENMKARAAELHGTIAWNPATSGGTKVVLQF
                     PMPENAG"
     gene            complement(556..702)
                     /locus_tag="VHE37_16885"
     CDS             complement(556..702)
                     /locus_tag="VHE37_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37263.1"
                     /translation="MFFPTENPTMLMRRALCAIWFLVLVGCSRGEPDQNLHFARNGEM
                     LLRL"
     gene            complement(719..1609)
                     /locus_tag="VHE37_16890"
     CDS             complement(719..1609)
                     /locus_tag="VHE37_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HVT37264.1"
                     /translation="MKILCLLLLASAAPLAAASDAPADATLPDQPIAPAIAVDSAPPK
                     DERSHEWLKRDPDRYSTVMVASGLSAHRPMFVLPVTWSPDYHGRHTEVEFQISLKQRV
                     LDSNLYFGYTQKSFWQMYNKHESSPFRESNYNPEVFYRFTPDPVRYNHFGADFGLEHE
                     SNGRDVPYSRSWNRLYFAPFQAKGKYLAYLKVWYRLPEHSKTSPSDSGGDDNPDIARY
                     YGYTELHLQREFGGGQQVWAMLRGNPGTGKGAISLNYSIPNSSHSMIWCLNMWHGYGE
                     SLIDYNHSITRVGLGLMFAR"
     gene            complement(1675..2289)
                     /locus_tag="VHE37_16895"
     CDS             complement(1675..2289)
                     /locus_tag="VHE37_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HVT37265.1"
                     /translation="MDEQSKSERIIELARKFGVLRPKDLVPFGIQAEYLRRLCDKGMI
                     KKVGRGSYVMSDTEMSNTLSLAIVGRAVPNGVVCLQSALAFHGIGNQPSNQVFVAIER
                     RAARPRVDYPEVRVARLGGPAFTEGVETHTIENVKLKIYSLEKTLADLFKFRNKIGPH
                     IAVDALRAAMNMEAGLNMKKLWHYAKLCRVERVMKPYVDALAMG"
     gene            2445..>3354
                     /locus_tag="VHE37_16900"
     CDS             2445..>3354
                     /locus_tag="VHE37_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HVT37266.1"
                     /translation="MLIDIAIFLAAAVLFVPLFRRAGLGAVLGYLAAGVVIGPHGAQL
                     ITGVADILDFSEFGIVLLLFVIGLELQPSRLWTLRRAVFGFGGAQVAATTLLLTLAAI
                     ALGVALNTAVVAGFGLAMSSTAFVLQMLAERNELASRHGRASFAILLFQDLSVIPFLA
                     ALPLLAAENSSAHHPLQRVMLVAAVFALVVLGGRYALRPAFRAIASSRTPEVFTAAAL
                     LIVIAIALLMEKIGLSMSLGAFTAGVLLADSEYRHELQADIEPFKGLLLGLFFIAVGM
                     STDIGLLLREPLTVLLLVTGLLLAKGA"
BASE COUNT          546 a         1046 c         1071 g          691 t
ORIGIN      
        1 gtgggagcag gaggaactgc ccgatccctc gctcgacgaa ggccacgcgc tgcacctgtt
       61 ccgcatcatg cgcgaggcgg tgaccaacgc gctgcgccac ggccaggcca cgcgtgtgcg
      121 cgtgcgcgtg aagcgccatg ccgccatgct gatgatggac gtggccgacg acggccccgg
      181 cgcgccgttg cccgacgccg atccgggccg cggcatggag aacatgaagg cgcgcgccgc
      241 cgagctgcac ggcaccatcg cttggaaccc ggcgacctcc ggtggcacca aggtggtgtt
      301 gcagttcccg atgccggaaa acgcgggcta ggttttgcgg cttggtagag gcgatttttg
      361 ccttcaccgc acccgcctat tctttgttat taaatgttta tgaaaaattt aaatatataa
      421 ttttttataa catcaattct gccactgcgg tatcgacctt ttgcgggcgc gggcggcccg
      481 caaacatccc tcaaggctat tccacgaggg gcaatcgggg ctcggcgttc ttgcccttgc
      541 ccacatgggc caccgctaca gtcgcagcag catttcaccg ttgcgggcga agtgcaggtt
      601 ctgatccggc tcgcctctgc tgcagcccac cagcaccagg aaccagatcg cgcagagcgc
      661 gcggcgcatg agcatggtcg ggttctcggt cggaaaaaac atgtacagag ttttcagctc
      721 accgcgcgaa catcaacccc agccccacgc gcgtgatcga atggttgtag tcgatcaggc
      781 tctcgccgta gccgtgccac atattcaggc accagatcat cgagtggctg gagttgggaa
      841 tgctgtagtt gagcgagatg gcgcccttgc cggtgcccgg gttgccgcgc agcatggccc
      901 acacctgctg gccgccgccg aactcacgct gcaggtgcag ctcggtgtag ccgtagtagc
      961 gcgcaatgtc cgggttgtcg tcgccgccgg agtcgctcgg cgaggtcttg ctgtgctccg
     1021 gcaggcgata ccagaccttc aggtatgcca ggtacttgcc cttggcctgg aacggcgcga
     1081 agtagagccg gttccagctg cgcgaataag gcacgtcgcg gccgttggat tcatgctcca
     1141 ggccgaagtc ggcgccgaaa tggttgtagc gcaccgggtc gggcgtgaag cggtagaaca
     1201 cttccgggtt gtagttgctc tcgcggaacg ggctggactc atgcttgttg tacatctgcc
     1261 agaacgattt ctgcgtgtag ccgaagtaga gattgctgtc gagcacgcgc tgcttgagcg
     1321 agatctggaa ttccacctcg gtgtggcggc cgtggtaatc cggcgaccag gtgaccggca
     1381 gcacgaacat cgggcggtgc gccgacagtc cgctggcgac catcacggtg gagtagcggt
     1441 cgggatcgcg cttgagccac tcgtgcgagc gctcgtcctt cggcggcgcg ctgtccaccg
     1501 cgatggcggg cgcaatcggc tggtcgggca gcgtggcgtc ggccggggca tcggaagcgg
     1561 cagccagcgg cgcggctgat gcaagcagca ggaggcagag gattttcatg ggcgtcagaa
     1621 aaaagatgcc cgcgcaaggc gggcacgaac aggcaattta ccctcaagac acggtcagcc
     1681 catcgccagc gcatcaacgt acggcttcat cacgcgctcg acgcggcaca gcttggcgta
     1741 gtgccacagc ttcttcatgt tcagcccggc ttccatgttc atcgccgcgc gcagcgcatc
     1801 gaccgcgatg tgcgggccga tcttgttgcg gaacttgaac aggtcggcca gggttttttc
     1861 cagcgaataa atcttgagct tcacgttctc gatggtgtgc gtctccacgc cctcggtgaa
     1921 cgccgggccg cccaggcgcg ccacgcgcac ttccgggtag tcgacccgcg gccgcgccgc
     1981 acggcgctcg atggcgacga acacctggtt cgaaggctgg ttgccgatgc cgtggaacgc
     2041 gagcgccgat tgcaaacaca cgacgccgtt gggcaccgcg cggccgacga tggcgagcga
     2101 cagggtgttg ctcatctccg tgtcggacat cacgtagctg ccgcgcccga ccttcttgat
     2161 catccccttg tcgcacaggc ggcgcagata ctccgcctgg atgccgaatg gcaccagatc
     2221 tttcggtcgc aggacgccga acttcctcgc gagttctatg atgcgttctg atttgctttg
     2281 ttcgtccatg tgtgaccaag cctagataga acactagatg tgtcgatatt taaaaacatt
     2341 ttagtgccga ttgcaatacc tcggctattt attatttaca tcaagacttt agcacaactt
     2401 attggaacgc ttggatttct ccctcgcgtt tttgagcgca gacgatgctg atcgacattg
     2461 ccatctttct cgccgccgcg gtgctgttcg tgccgttgtt ccgccgtgcg ggactcggtg
     2521 ccgtgctcgg ctatctcgcc gcaggcgtgg tgatcggtcc gcacggcgcg cagctgatca
     2581 ccggcgtggc cgacatcctc gacttctccg aattcggcat cgtgctgctg ctgttcgtca
     2641 tcggtctcga actgcagccc tcgcgtttgt ggacgctgcg ccgcgcggtg ttcggcttcg
     2701 gcggcgcgca agtggcggcg accacgctgc tgctgacgct ggcggcgatt gcgcttggcg
     2761 tcgcgttgaa cacggcggtg gtcgcaggtt tcggtctcgc catgtcgtcc actgcattcg
     2821 tgctgcagat gctcgccgag cgcaacgagc tcgccagccg ccatggccgc gcgtcgttcg
     2881 ccatcctgct gttccaggat ctttcggtga tcccgttcct ggccgcgctg ccgctgctgg
     2941 cggcggagaa ctcgtccgcg catcacccgc tgcagcgggt gatgctggtc gcggcggtct
     3001 tcgcgctggt ggtcctcggt gggcgctatg cgctgcggcc ggcattccgc gccatcgcgt
     3061 cgtccaggac gccggaagtg ttcaccgccg cggcgctgct catcgtcatt gccattgcat
     3121 tgctgatgga gaagatcggc ctgtcgatgt cgctcggcgc cttcaccgcc ggcgttctgc
     3181 tggccgactc cgagtaccgc catgagctgc aggctgacat cgagccgttc aagggcctgc
     3241 tgctgggcct gttcttcatc gcggtgggca tgtccaccga tatcggcctg ctgctgcgcg
     3301 agccgctcac cgtgctgctg ctggtgaccg gcctgctgct ggccaagggt gccg
//
LOCUS       DASZZC010000783         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_125228,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000783 DASZZC010000000
VERSION     DASZZC010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125228"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="VHE37_16905"
     CDS             <1..1559
                     /locus_tag="VHE37_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HVT37267.1"
                     /translation="TFQTPTPPKRGRRPTLHLEPEPISTGAEWSFELIEKYDYAIHDI
                     AANEFGLDTYPNQIEVIASEQMLDAYASLGLPIGYPHWSYGKEFIKNEQMYRKGARGL
                     AYEIVINSNPCIAYLMEENSMTMQALVIAHACYGHNSFFKGNYLFRQWTNADAIIDYM
                     AFARKYVMDCEERHGMGAVEEILDSCHALMSHGVDRYRHPTPLSVEREQARLRDREEH
                     EWKRHDEIWRTVPKKDKGKGKHHKAQVHFPPEPQENILYFVEKYSPRLEPWQAELVRI
                     VRKLSQYFYPQGLTKVMNEGWATFWHYTLLNRLFDKKLVDAGFMLEFLSSHTNVITQR
                     GFDQKGYGGINPYALGFAMMSDIRRICESPTPEDRQWFPDIAGGDWRKVLDFAMRNYK
                     DESFIGQFLSPRLMREFHLFAVGDRASENELVVDAIHNEQGYRRLRQMFAAQYNRDTQ
                     VPDIQVLRYDRRGDRSLTLRHQRHRGRPLNEEAGEVLKHLARLWGFTVKLETWDEGGK
                     VSEQSETRGY"
     gene            complement(1690..>3649)
                     /locus_tag="VHE37_16910"
     CDS             complement(1690..>3649)
                     /locus_tag="VHE37_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37268.1"
                     /translation="VDRLPAASAAHRAWVYRFVRPGRLRPPARVDYVNGAGACPADGM
                     SNVLGEIHPYDPMQVAHRYCGASFSYTHDGAVQQETLLNMLGAPLQALTYTAPDLAQL
                     TQQGFEAPSAGTGVRYVQFTRDAHGRDVRVQFLYARGVPRMSRAGEYGYVAAYDDHDR
                     LLSRSVLDDRGVEVGEVTRYAYNEHGVCLETRTEDGHGAPRAVDGYVSVIQEPDAWGN
                     ALVTAWVDGAGHPALTASGYASRSDRYDAQGNNTDTCYFDERGQPAEGDRGAECEHDT
                     FDQHGRVVRSDLTGADGHRRQNAEGEAVVEFDYDARGNVIENRLYDGAGHLTEGKFGA
                     GQHNVFDERGYLLRQDFFGADHQPEMTALGSTILTTVDERGNISEFRFFDTQGRPLVR
                     PDKGYAVQRVLRDERGNVITRLFLGTDDKPVANIEGYASREMRYDDAGNEVERRMYDI
                     SHELTRDRDGVAVYRDRYDRLGRKIEERYFDEHEQPAPVQDGSYGYRADYDARGRMTT
                     KVFLDAHGQVARLPALGYAGVRYRYGAQNRVAQEDFLDVDGQPVQHGAVAGMQHRYND
                     YGSEIERRWLDAQGHAVALPTTGCAAELFEFDRYQDPVVRRCVSADGAPLNRRDGGWA
                     VKRVTRDHGVEVKIEYFDAAGNPVTPRK"
BASE COUNT          621 a         1208 c         1216 g          604 t
ORIGIN      
        1 gcaccttcca gacgccgacg ccgcccaagc gcgggcgccg cccgacgctg cacctggagc
       61 cggagccgat ctccaccggc gccgagtgga gcttcgagct gatcgagaag tacgactacg
      121 ccatccacga catcgcggcc aacgaattcg gcctcgacac ctatcccaac cagatcgagg
      181 tgatcgcgtc ggaacagatg ctcgacgcct acgcttcgct cggcctgccg atcggctatc
      241 cgcactggag ctacggcaag gagttcatca agaacgagca gatgtaccgc aagggcgcgc
      301 gcggcctggc gtatgaaatc gtcatcaaca gcaacccgtg cattgcctat ctcatggaag
      361 agaacagcat gaccatgcag gcgctggtca tcgcccacgc ctgctacggg cacaactcct
      421 tcttcaaggg caattacctg ttccgccagt ggaccaatgc cgacgccatc atcgattaca
      481 tggcgttcgc gcggaaatac gtgatggact gcgaagagcg ccatggcatg ggcgcggtgg
      541 aggagatcct cgactcctgc catgcgctga tgagccatgg cgtggatcgc taccgccacc
      601 cgacgccgct gtcggtggag cgcgaacagg cgcgcctgcg cgaccgcgag gagcacgagt
      661 ggaagcgcca cgacgagatc tggcgcaccg tgccaaagaa ggacaagggc aagggcaagc
      721 accacaaggc gcaggtgcat tttccgcccg agccgcagga gaacatcctg tacttcgtcg
      781 agaagtactc tcccaggctg gagccgtggc aggccgagct ggtgcgcatc gtgcgcaagc
      841 tgtcgcagta cttctacccg cagggcctga ccaaggtgat gaacgagggc tgggctacct
      901 tctggcacta cacgctgctc aaccggctgt tcgacaagaa gctggtcgac gccggcttca
      961 tgctcgagtt cctgtcctcg cacaccaatg tcatcaccca gcgcggtttt gaccagaagg
     1021 gctacggcgg catcaacccc tatgcgctgg gcttcgccat gatgagcgac atccgccgca
     1081 tctgcgaaag ccccacgccc gaagaccgcc agtggtttcc cgacatcgct ggcggcgact
     1141 ggcgcaaggt gctggacttc gccatgcgca actacaagga cgaatcgttc atcggccagt
     1201 tcctcagccc gcggctgatg cgcgagttcc acctgttcgc ggtgggcgac cgtgccagtg
     1261 aaaacgaact ggtggtcgac gccatccaca acgagcaggg ataccgccgc ctgcgccaga
     1321 tgttcgccgc gcagtacaac cgcgacaccc aggtgcccga catccaggtg ctgcgctatg
     1381 accgccgcgg cgaccgctcg ctcacgctgc gccaccagcg ccatcgcggg cggccgctca
     1441 acgaagaggc cggcgaagtg ctcaagcacc tggcgcgttt gtggggtttc accgtgaagc
     1501 tggaaacctg ggacgagggc ggcaaggtgt cagagcagag cgagacgcgc ggctactgat
     1561 tattttttgc ctcaccgcac cccggtttgc gctgttataa aaaattttct aaaagaaaaa
     1621 acagacatta atttataaca gcgcagatgc cggtgcggtg gaggcaaaaa atgccggcag
     1681 ccatggcggc tacttgcgcg gcgtcaccgg gttgccggcg gcgtcgaagt attcgatctt
     1741 cacctcgacg ccgtggtcgc gcgtcacccg cttcaccgcc cagccgccgt cgcggcggtt
     1801 gagcggcgcg ccgtcggccg acacacagcg ccgcaccact gggtcctggt agcggtcgaa
     1861 ctcgaacagc tcggccgcgc agccggtggt cggcagcgcg acggcatggc cctgtgcatc
     1921 gagccagcgc cgctcgatct cgctgccgta atcgttgtag cgatgctgca tgccggcaac
     1981 ggcgccgtgc tggaccggct ggccgtcgac atcgagaaaa tcctcctgcg ccacgcggtt
     2041 ctgcgcgccg tagcggtagc gcacgccggc gtaacccagc gccggcaggc gcgccacctg
     2101 gccgtgcgca tcgaggaaca ccttggtggt catgcggccg cgcgcgtcgt agtcggcgcg
     2161 gtagccgtag gagccatcct gcaccggtgc cggctgctcg tgttcgtcga aatagcgctc
     2221 ctcgatcttg cggccaagcc ggtcgtagcg gtcgcggtaa acggccacgc cgtcgcggtc
     2281 gcgcgtcaat tcgtggctaa tgtcgtacat gcggcgctcg acctcgttgc cggcgtcgtc
     2341 gtaacgcatt tcgcgcgagg catagccttc gatattggcc accggcttgt cgtcggtgcc
     2401 caggaacagg cgggtgatga cgttgccgcg ctcgtcgcgc aacacgcgct gcacggcgta
     2461 acccttgtcc ggccgcacca gcgggcggcc ctgcgtatcg aagaaccgga actcggaaat
     2521 gttgccgcgc tcgtccaccg tggtcaggat ggtgctgccc agtgcggtca tctccggctg
     2581 gtggtcggcg ccgaagaaat cctggcgcag caggtagccg cgctcgtcaa acacgttgtg
     2641 ctggccggcg ccgaacttgc cctcggtgag gtggccggcg ccgtcgtaca gccggttctc
     2701 gatgacgttg ccgcgggcgt cgtagtcgaa ttccaccacc gcttcgcctt cggcgttctg
     2761 gcggcggtgg ccgtcggcgc cggtgaggtc ggagcgcacc acgcggccgt gctggtcgaa
     2821 cgtatcgtgc tcgcactcgg cgccgcggtc gccttcggcc ggctggccgc gttcgtcgaa
     2881 gtagcaggtg tcggtgttgt tgccctgcgc gtcgtagcgg tcgctgcgcg aggcatagcc
     2941 gctggcggtc agcgccgggt ggccggcgcc atcgacccaa gccgtgacca gcgcgttgcc
     3001 ccaggcgtcg ggctcctgga tcacgctgac gtagccgtcg accgcgcgcg gcgcgccgtg
     3061 gccatcctcg gtgcgcgtct ccaggcaaac gccgtgctcg ttgtacgcgt agcgcgtgac
     3121 ctcgccgacc tcgacgccgc gatcgtcgag cacgctgcgg ctgagcaggc ggtcgtgatc
     3181 gtcgtaggcg gcgacatagc cgtactcgcc ggcgcggctc atgcgcggca cgccgcgggc
     3241 gtagaggaac tgcacgcgca cgtcgcggcc gtgggcgtcg cgcgtgaact gcacgtagcg
     3301 cacgccggtg ccggcgctcg gcgcttcgaa gccctgctgc gtcagctgcg ccaggtcggg
     3361 tgcggtgtag gtcagcgcct gcagcggcgc gccgagcatg ttgagcagcg tttcctgctg
     3421 cactgcgccg tcgtgcgtgt agctgaagct ggcgccgcag tagcggtgcg ccacctgcat
     3481 cgggtcatat ggatggatct cgccgagcac gttgctcatg ccgtcggcgg ggcaggcgcc
     3541 ggcgccgttg acgtagtcga cgcgcgcggg cggacgcagc cggccggggc gcacgaagcg
     3601 gtagacccac gcgcggtgtg cggcagaagc agccggcagg cgatcaacg
//
LOCUS       DASZZC010000784         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_887422,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000784 DASZZC010000000
VERSION     DASZZC010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887422"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..>1713
                     /locus_tag="VHE37_16915"
     CDS             55..>1713
                     /locus_tag="VHE37_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37269.1"
                     /translation="MDINSGGSSLTTGSINLQAAAGDFVLGQLTATHGGIDLETGGNI
                     TTGDLAAQDVTTDSSAANADVVIFAGGSISTGAIASSARVLSGIADCVSGCGTASAKV
                     DLHSNSSFSAGATALSTHAITTQAVSGDADANASVTLTHHFTPSGVATGTIDTGRILT
                     SATSNATPDGSVHLVNETIGRINTGGASITATGYDVSGAAVMGAGDIELETGKDNITT
                     GALIATNSSISVTDGNFGGANHAADTQVRLGALSGGFDAQDNPYELHTVNLLSGGSLA
                     LDTSAGLIADDISLGAGDGDFSAGLLQATAGQVVLEARNGNITLTAAPLAAGCGNSSG
                     CVFIDTDHTMLIDTGNAPLVLDQVNMGTSSVFTSIIQLRSTAGITVSNSHLNGGVIDI
                     QGPQDVSVTGSLLTSECQVPSNCLHLVAGNDLLLQNTSVADISQIGLSAGRDLIFDHA
                     TLGYSNGTPLAWYYSTAASAGRDVLLRNRSSLSNAIVLLEAGRNVVDDGSGQASFINS
                     NSLGIGASGNIDLASTVITVSNGVAPIGGDPELVTLEQSQTDGID"
BASE COUNT          321 a          602 c          538 g          252 t
ORIGIN      
        1 acgcacggcg gattctatgc actgaccgct ctcaacctga tctccggtgg gcgcatggac
       61 atcaacagcg gcggcagctc gctgaccacc ggcagcatca acctgcaggc ggccgcggga
      121 gatttcgtgc tgggccagct gaccgccacg cacggcggca tcgacctgga aacgggcggc
      181 aacatcacca ccggtgacct ggccgcgcag gatgtgacca ccgacagcag cgccgccaat
      241 gccgacgtgg tgatcttcgc cggcggcagc atcagcaccg gcgccatcgc ctccagcgca
      301 cgcgtgctca gcggcatcgc cgactgcgtc tcgggatgcg gcaccgccag cgcgaaggtg
      361 gacctgcaca gcaactcgag cttcagcgct ggggcgaccg cgctgagcac gcacgccatc
      421 accacgcagg cggtttcggg cgatgccgat gcgaatgcca gcgtgaccct gactcatcac
      481 ttcacgccgt cgggtgtggc caccggcacc atcgacaccg ggcgcatctt gaccagcgcc
      541 acctcgaatg caacgccgga tggcagcgtg cacctggtca acgaaaccat cggtcgcatc
      601 aacaccggcg gcgcgtcgat caccgccacg ggctacgacg tcagcggcgc ggcggtcatg
      661 ggcgccggcg acatcgagct ggaaaccggc aaggacaaca tcactaccgg cgcgctcatt
      721 gccaccaaca gttcgatcag cgtcaccgac ggcaacttcg gcggcgccaa ccatgcggcc
      781 gacacgcagg tgcgcctcgg tgcgctctcc ggcggcttcg atgcgcaaga caacccctat
      841 gagctgcaca cggtcaacct gctgtcggga ggctcgctgg cactcgacac cagtgcaggg
      901 ctgatcgccg acgacatcag cctcggtgcc ggcgacggcg acttcagcgc cggcctgttg
      961 caggccaccg cgggccaggt ggtgctggag gcgcgcaacg gcaacataac tctgactgcc
     1021 gcgccgctcg ccgcgggctg cggcaattcg agcggttgcg tgttcatcga taccgatcac
     1081 accatgctga tcgacaccgg caatgcgccg ctggtgctcg accaggtgaa catgggcacc
     1141 agttcggttt tcaccagcat catccagctg cgcagcacgg ccggcatcac tgtcagcaac
     1201 agccacctga acggcggcgt catcgacatc cagggaccgc aggatgtctc ggtgaccggc
     1261 agcctgctga ccagcgagtg ccaggtgccg agcaactgcc tgcacctggt ggcgggcaat
     1321 gacctgctgc tgcagaacac ctccgtcgcc gacatcagcc agatcggcct gtccgcgggc
     1381 cgcgacctga tcttcgacca tgccacgctc ggctacagca acggcacgcc gctggcctgg
     1441 tactacagca ccgcggcgag cgccggccgc gacgtgctgc tgcgcaaccg cagttctttg
     1501 tcgaacgcca ttgtgctgct tgaggctggg cgcaatgttg tcgacgacgg cagcggccag
     1561 gccagcttca tcaacagcaa ttcgctgggc atcggcgcca gcggcaacat cgacctggcg
     1621 tcgaccgtca tcaccgtgtc caatggcgtg gcgccgatcg gcggcgaccc ggaactggtg
     1681 acgctggaac aatcgcagac cgatggtatc gac
//
LOCUS       DASZZC010000785         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_443608,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000785 DASZZC010000000
VERSION     DASZZC010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443608"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VHE37_16920"
     CDS             complement(<1..1429)
                     /locus_tag="VHE37_16920"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HVT37270.1"
                     /translation="MSERQGVVISGVDRAWLEMDAPANPMVVNAVIEFEPPVNEYRLC
                     AEMVERILRHPRFRQRAEMEPAPPRWVDDEAFNVEYHSQVIPPQQFPSDAMLRAAIEA
                     QVSQPLDRTMPLWRLFVYARPDRSATVLFRAHHGLADGIALVQMLMQCLDGAPKKNGA
                     APAHEPRTRHHGPLGKLIDRLEWVNAMAERVGDFALADLRHPEWIPGQLREARDMATA
                     VTHVLALPEDNPRELCRPLQARRSVAWAGDIPLAPVKRLAHATGAKVNDVFLAAVAGA
                     FNRYLQVHGAALKQTQNLRVSVPVNLRPAGEQGLGNCFGLVLVDLPVGVRDWQQRLEL
                     VRQRMEQLKHSPTARVVLFSLAAAGHLPASFEKQLVRRVAAKSAAVVSNLRGPARAMA
                     LAGMRIRTLAFWPPAAETIGVGLSLFSFNRHITVGICADRGVVANAQSLLDALREELR
                     AMSGATRRPRKPVARKAALERPAAPA"
     gene            complement(1716..2606)
                     /locus_tag="VHE37_16925"
     CDS             complement(1716..2606)
                     /locus_tag="VHE37_16925"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT37271.1"
                     /translation="MFEMLKLRILRRLIRLAIRYKWRGKAPQDLAVSNETLPVDGGRI
                     GVRIYTPRGAGPFPVLHFFHGGGWVGFDLDTHDALCRDLCVRSGYLVLAFDYRLAPEH
                     PFPIPVHDCLASLGWTRDHAARLNGDAQRVVLCGDSAGGNLAAVAAQQARTLHPGLVK
                     GQVLIYPVTDHCAHAQWPSYRTYGTAKYALSHPKMVELWELYMRGGAPWPAGATSHEL
                     ATPLHTADLRGLPPTLVVLAVEDLLHDEGLAYAQRLAAAGVPVTVREYPGQQHGFVGT
                     EPSVAHDQAVADIAAWLQDR"
     gene            complement(2620..>3847)
                     /locus_tag="VHE37_16930"
     CDS             complement(2620..>3847)
                     /locus_tag="VHE37_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT37272.1"
                     /translation="ANQSSSLMQELRLTSTGDGRYKWLAGAFYRKVHLTETDDILASN
                     QALPIPASLLGALEGALPGLGGTVTPDGKLNTAQSIADPLDVTEKALFGEATATFWSD
                     LEITLGLRAFRTESASRVLFSGVLATNPQNINGLLQSTRAGDMRSQGVNPKFSIKYRF
                     DPHASVYAAVTKGFRFGGPQVLIGTLTSSAPAFYKSDTLWSYELGLRTQWLDNTLIAD
                     VTPYLIDWINPQLQQADATGLGSYFDNVGGARSTGVEAALRYLTPLPGLALAFSGSYA
                     NTVTTKPFTTSSGVHTQPGTRWPLAAPWQSATTLAYSHPLFGWEAGASLLYATISSAP
                     NTLAYLDSVFGYKTLDLTLSAANPELGGRPELALSLTNATDVRGIISGVNNPQFGKDH
                     NYIRPRTLLARVSFAF"
BASE COUNT          599 a         1306 c         1344 g          598 t
ORIGIN      
        1 aggcgggtgc agccgggcgc tccagcgcag ccttgcgcgc gaccggcttt cgcgggcggc
       61 gcgttgcgcc ggacatggcg cgcagttctt cgcgcaatgc atcgagcagc gactgcgcat
      121 tggccaccac gccgcggtcg gcgcagatgc ccacggtgat gtggcgattg aagctgaaca
      181 gcgacaggcc cacgccgatg gtctcggcgg ccggcggcca gaacgccagc gtgcggatgc
      241 gcatgccagc gagtgccatg gcgcgggcag ggccgcgcag gttcgacacc accgcggcag
      301 acttcgccgc cacgcggcgc accagctgct tctcgaacga cgccggcaga tggccggccg
      361 cggccaggct gaacagcacc acgcgcgcag tcggcgaatg cttgagttgt tccatgcgct
      421 ggcgcaccag ttccagccgc tgctgccagt cgcgcacgcc caccggcagg tccaccagca
      481 ccaggccgaa gcagttgccc aggccctgct cgcccgccgg gcgcaggttc accggcaccg
      541 acacgcgcag gttctgcgtc tgcttgagcg cagcgccgtg cacctgcagg tagcggttga
      601 aggcgccggc caccgcggca aggaagacgt cgttgacctt ggcgccggtg gcatgcgcca
      661 gccgcttcac cggcgccagc ggaatgtcgc ccgcccacgc cacgctgcgg cgcgcctgca
      721 gcggccggca cagctcgcgc ggattgtctt ccggcagcgc cagcacatgc gtcaccgccg
      781 tggccatgtc gcgcgcctcg cgcagctggc cggggatcca ttcgggatgg cgcaaatcgg
      841 ccagcgcgaa atcgcccacc cgctcagcca tggcgttgac ccattcgagg cggtcgatca
      901 gcttgcccag cgggccgtgg tgccgggtgc gcggctcgtg tgcgggcgcg gcgccattct
      961 tcttcggcgc gccgtccagg cactgcatca gcatctgcac cagcgcgatg ccgtcggcca
     1021 ggccgtggtg cgcgcgaaac agcacggtgg cgctacggtc cgggcgtgca tacacgaaca
     1081 gccgccacag cggcatggtg cggtccagcg gctgactcac ctgcgcctcg atcgccgcgc
     1141 gcagcatggc gtcggaagga aactgctgcg gcgggatcac ctgcgagtgg tactcgacgt
     1201 tgaacgcctc gtcgtccacc cagcgcggcg gcgccggttc catctcggcg cgctggcgga
     1261 agcgcggatg gcgcaggatg cgctcgacca tttccgcgca cagccggtac tcgttcaccg
     1321 gcggctcgaa ctcgatcacc gcgttgacca ccatcgggtt ggcgggcgca tccatctcca
     1381 gccacgcgcg gtcgacgccg ctgatcacca cgccctgacg ctcgctcatg tctgcaccct
     1441 gtctgccggt gccggcatta cgcgcgtgca ggcgcaggca cgcgttgatc cacgtcaatg
     1501 ccgtttgcgg ctgcgcgcga tggcatccgc gaagccaaaa aagccgccac cgcaccagca
     1561 ctttgtctgt tattaattat tgttcaatta caaaataata atatatttaa taacagggtc
     1621 aggccgggtg cggtagggcc atttccaggc gcaggtctcg ccatgcgccg gcccggacgg
     1681 ccagccgccg tgctacgaaa tcagtagcag cggggctagc ggtcctgcag ccacgccgcg
     1741 atgtcggcca ccgcctggtc atgcgcaaca ctgggctcgg tgccgacgaa gccatgctgt
     1801 tgccccggat attccctgac cgtgaccggc acgccggccg cggccaggcg ctgcgcatag
     1861 gccaggccct cgtcgtgcag caggtcctcc accgccagca ccaccagcgt gggcggcagg
     1921 ccgcgcaggt cggcggtgtg cagcggcgtg gccagttcgt gcgaagtggc gccggccggc
     1981 cagggcgcgc caccgcgcat gtacagctcc catagctcaa ccatcttggg atgagacaac
     2041 gcgtacttcg cggtgccgta ggtgcggtac gacggccact gcgcgtgcgc gcaatgatcg
     2101 gtcaccgggt agatcagcac ctggcccttg accagcccgg gatgcagcgt gcgcgcctgc
     2161 tgcgcggcca cggcggcgag attgccgccc gcgctgtcgc cgcacagcac cacgcgctgc
     2221 gcatcgccgt tgaggcgcgc ggcgtggtca cgggtccagc ccaatgacgc gaggcaatcg
     2281 tgcaccggga ttggaaacgg atgctcgggc gccaggcgat agtcgaatgc cagcaccagg
     2341 tagccgctgc gcacgcacag gtcgcggcac agtgcatcgt gggtgtcgag atcgaagccg
     2401 acccagccgc cgccgtggaa gaaatgcagc accgggaacg gtccggcacc gcgcggcgtg
     2461 tagatgcgca cgccgatgcg gccgccgtcc accggcagcg tctcgttgct caccgccagg
     2521 tcctgcggcg ccttgccgcg ccacttgtag cggatcgcca ggcggatcag ccggcgcagg
     2581 atgcggagct tgagcatctc gaacatgggt atccccgttt cagaatgcga acgacacgcg
     2641 cgccagcagc gtgcgcggac ggatgtagtt gtggtccttg ccgaactgcg ggttgttcac
     2701 gccgctgatg atgccgcgca catcggtggc attggtcagc gacagcgcca gctccggccg
     2761 gccgccgagt tccgggttgg ccgcgctcag cgtcaggtcc agcgtcttgt agccgaacac
     2821 gctgtcgaga tacgccaggg tgttcggcgc cgagctgatg gtggcataca gcaggctggc
     2881 gccggcttcc cagccgaaca gcgggtgcga gtaggcgagc gtggtggccg actgccacgg
     2941 cgccgccagc ggccagcgcg tgcccggctg cgtgtgcacg ccgctgctgg tggtgaaggg
     3001 cttggtggtc accgtgttgg catacgagcc ggagaaggcc agtgccaggc cgggcagcgg
     3061 cgtgagataa cgcagcgccg cttccacgcc agtgctgcgc gcgccgccga cgttgtcgaa
     3121 gtacgagccc agcccggtgg catcggcctg ctgcagctgc gggttgatcc agtcgatcag
     3181 gtacggcgtg acgtcggcga tcagcgtgtt gtccagccat tgcgtgcgca gtcccagctc
     3241 gtagctccac agcgtgtcgg acttgtagaa cgcgggcgcg gacgaggtga gcgtgccgat
     3301 cagcacctgc ggtccgccga agcggaagcc cttggtcacg gcggcataca cgctggcgtg
     3361 cgggtcgaag cggtacttga tggagaactt ggggttgacg ccctgcgagc gcatgtcgcc
     3421 ggcgcgcgtc gactgcagca ggccgttgat gttctgcggg ttggtggcca gcacgccgga
     3481 aaacagcacg cggcttgccg actcggtgcg gaacgcgcgc agccccagcg tgatctccag
     3541 gtcgctccag aacgtcgcgg tggcttcgcc gaacagcgcc ttctcggtga cgtcgagcgg
     3601 atcggcgatg ctctgcgccg tgttgagctt gccgtcgggc gtgaccgtgc cgcccagtcc
     3661 cggcagtgcg ccttccagcg cgccgagcag cgacgccggt atcggcaggg cctggttgct
     3721 ggccaggatg tcgtcggtct cggtcaggtg caccttgcgg tagaacgcgc cggccagcca
     3781 cttgtagcgg ccatcgccgg tggaggtgag gcgcagttcc tgcatgaggc tcgatgactg
     3841 gttggcc
//
LOCUS       DASZZC010000786         4616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_703899,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000786 DASZZC010000000
VERSION     DASZZC010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703899"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..510)
                     /locus_tag="VHE37_16935"
     CDS             complement(256..510)
                     /locus_tag="VHE37_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37273.1"
                     /translation="MSAQPQIPVIAPVPSERRSPRAQTPRRTRARAHTRRRRSVALEQ
                     PLLQRWWNEHAVKSGMVVFYAWLAVANYGRVQHLLAQVFS"
     gene            604..1269
                     /gene="lexA"
                     /locus_tag="VHE37_16940"
     CDS             604..1269
                     /gene="lexA"
                     /locus_tag="VHE37_16940"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVT37274.1"
                     /translation="MSELTDRQSEILRFVRDCMQEHGAPPTRADIARAFGFSSPNAAE
                     RHLRALAAKGHIELSPGASRNIRLSGSAQEALAAPYQLPLVGRIAAGSPITAPDNIDE
                     QVMIDPGLFHPRADFLHRVSGHSMREAGILDGDLVGIHHQESADNGQIIAAVLFDRRA
                     GDDRITLKRYSRRGHRVTLRSENSEPGYEPIEIDLAAASEDEERPQFRIAGIYAGLLR
                     RAR"
     gene            complement(1266..2057)
                     /locus_tag="VHE37_16945"
     CDS             complement(1266..2057)
                     /locus_tag="VHE37_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT37275.1"
                     /translation="MSTMNPYGRNFGAAAADYARHRAGVPDSLFERLAQLGVGLPRQC
                     ITDIGTGTGMLGRGFARRGARVIGVDPDARMLEQARELDRAAGVASEYRVGRAEQLPL
                     DDDALDVISAAQCWHWFQPELAAREMARACRASGRVAIVHFDWLPLPGSLVEATERLI
                     EAHNPKWDLGGGDGFHPESLPYLRAAGFGAFEQFSYEVDVPYTPEGWRGRIRASAGVG
                     ASLSPETIAAFDEEHARVIAQRFAGETVLAPHRVFAIVGERLGKI"
     gene            2346..>4616
                     /locus_tag="VHE37_16950"
     CDS             2346..>4616
                     /locus_tag="VHE37_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37276.1"
                     /translation="MLKISAARNAWLFVIAFSVAAASTIGGGAWLQARSPMQKLAAAP
                     CTDPASVQKDGDGDGDADAGTRCAPRRHPESAREFARMGDAWSMRTGQPPDGAIRAAL
                     AQKAAMVQTKSAISNAGGTWQAYGQGPLIFNDPAYGQVNGEGMVNVSGRVDAFAYDPA
                     TLRVFALPGNGGVWTSTDFGQNWSSIGDRLPTQNNGAVAWTSSNGGTLIVLSGNSDFL
                     GSPSYGGLGAFWTNDLGQTWNQSVGIPDGIYAFELAVDHAHPDIVYAATSRGLFRSTD
                     AGRHFVNVALPTSTDCAGRTDLGKCQFANVVTSVVVQEPGGTSNVAGGAVLAAVGFQE
                     GNTNTFSDGTLVSPGNGLYASPDGAPGSFTKLAVGAASAVSPVGFAPDNRIGRVAMAV
                     AHGDDQDHNYVYALVQDALLMTGGLPYIDVNPTSLGAPVAVPIPTVLNGLYISSDFGQ
                     SWTRAFDFVASQNPASGSALAGLQQATGYGPGVQAWYNLWVESDPTVTASNAPSRLFF
                     GLEEVWSSLTASVPQNGTAQLADVVNYHVIGAYYAGTTCGALNLGAIFPNLPPACPGR
                     NPPTATTTTHPDQHGHLVMPDGQGGVYLFAGSDGGVFVQHHAAGENFDNAGWGDGAQN
                     GFNTLLPYDIAVAKDNTVWFGLQDNGSGKIDPVTQKVYETYGGDGFWMAVDPDNSNTA
                     YEEYTNGDISVTTDGGQTWTDMNPNLANPNFANQFYMDPTDAKHILTGGSDIVDTVDG
                     PDTCATNTCTWATLFTL"
BASE COUNT          785 a         1626 c         1496 g          709 t
ORIGIN      
        1 gttgagttcg ccgtcgagct gcgccagcgc gaggatgcgc ggcaggtccg cgccttcggc
       61 ggcgcggctc tcggatttgc gcagcaggaa acccagcacg cccagtgcca gcgccagtgc
      121 cagcaccacc accacctgtc ctctcatctc aatcccccgc cggccgactg cgcggccttg
      181 atttccagtg cctgcacttc attgcaggcc tcgtgccatg cggcacgggc gcgggggact
      241 atagcgcaag ccgcctcagc tgaagacctg ggccagcagg tgctggaccc gcccgtaatt
      301 ggccacggca agccaggcgt aaaacaccac catgccggac ttcaccgcgt gctcgttcca
      361 ccagcgctgc agcagcggct gctccagcgc aacactgcgg cggcgccgcg tgtgggcgcg
      421 ggcgcgcgtg cgccgcgggg tctgcgcgcg cgggctgcgg cgctcggaag ggacaggagc
      481 gatgacgggg atctggggct gggcggacat ggggtgctcc tgcttggctt tcgtggtcga
      541 acgtgtatac ttatacacta gtggatggat gaacaccagc cctgacgcaa cccgggagcg
      601 gcgatgagtg aactgactga tcgacaaagc gagatcctgc gcttcgtgcg cgactgcatg
      661 caggagcacg gcgcgccgcc cacgcgcgcg gacatcgcgc gcgccttcgg cttctcctcg
      721 cccaacgcgg cggagcgcca tctgcgcgcg ctggcggcca agggccacat cgaactgtcg
      781 cccggcgcct cgcgcaacat ccgcctctcc ggttcagcgc aggaagcgct ggccgcgccg
      841 taccagctgc cgctggtcgg gcgcattgcc gccggctcgc cgatcaccgc gccggacaat
      901 atcgacgagc aggtgatgat cgaccccggc ctgttccacc cgcgcgcgga tttcctgcac
      961 cgcgtcagcg gccactcgat gcgcgaggcc ggcatcctcg acggcgacct ggtcggcatc
     1021 catcaccagg aaagcgccga caacggccag atcatcgccg ccgtgctgtt cgaccgccgc
     1081 gccggcgacg accgcatcac gctcaagcgc tactcgcgcc gcggccaccg cgtgacgctg
     1141 cgctcggaga acagcgagcc cggctacgag ccgatcgaaa tcgacctggc agccgccagc
     1201 gaagacgagg agcggccgca gttccgcatt gccggcatct atgccggcct gcttcgccgc
     1261 gcgcgctaga tttttcccag gcgctcgccg acgatggcga acacgcggtg cggcgccagc
     1321 accgtctcgc cggcaaaacg ttgcgcaatg acgcgcgcgt gttcttcatc gaacgccgcg
     1381 atggtctccg gcgacaggct ggcgccgacg ccggcactgg cgcggatgcg gccgcgccag
     1441 ccttccggcg tgtacggcac gtcgacttca taggaaaact gctcgaacgc gccgaagccc
     1501 gccgcgcgca ggtacggcag gctctccggg tgaaaaccgt cgccaccgcc caggtcccac
     1561 ttcgggttgt gcgcttcgat cagccgctcg gtggcttcca ccaggctgcc gggcagcggc
     1621 agccagtcga agtgcacgat ggccacgcgg ccgctcgcgc ggcacgcgcg cgccatctcg
     1681 cgcgcggcga gttccggctg gaaccagtgc cagcactgcg cggcgctgat gacatcgagc
     1741 gcatcgtcgt cgagcggcag ctgctccgcg cgccccacgc ggtattcgct ggccacgccg
     1801 gcggcgcggt cgagttcgcg cgcctgctcc agcatgcgcg cgtcgggatc gacaccgatg
     1861 acgcgggcgc cgcgccgcgc aaagccgcgg ccgagcatgc cggtgccagt accgatgtcg
     1921 gtgatgcatt gccgcggcaa gccgacgccg agttgcgcca ggcgttcaaa cagggaatcg
     1981 ggcacacccg cgcggtggcg cgcgtaatcc gccgccgccg caccgaagtt gcggccgtag
     2041 ggattcatcg tgctcatctg tgcactccgc ttcgccatca cgcccggcgc attgttccag
     2101 cgcccgaatc aaggaccacc gcacggccat gatgcatgtt attaaatata tgtttatttt
     2161 tactttataa tatatttaat aacatgccca cgcagggtgc ggtatagaga aaaaaaccac
     2221 gccagcgatt ttgcgcgagg tgggaagtaa agatgcgttg cgccggtgtc gctattaaca
     2281 ttttgctgag catctgtgcg tgccgctcgc agataatcgc gctgactacc ctggggggtt
     2341 gaaccatgtt gaagatttcc gccgcgcgca acgcgtggct tttcgtaatc gctttttccg
     2401 ttgccgcggc ctccaccatc ggcggcggcg cctggctgca ggcgcgcagc ccgatgcaaa
     2461 aacttgcggc ggcgccctgc accgatccgg cgtccgtaca gaaggacggc gatggtgacg
     2521 gcgacgctga tgccggtacg cgctgcgcgc cgcggcgcca cccggaatca gcgcgtgaat
     2581 ttgcgcgcat gggtgatgcc tggagcatgc gcaccggcca gccgcctgat ggcgccatcc
     2641 gcgccgcgct ggcacagaag gccgcgatgg tgcagaccaa gtccgccatc agcaacgccg
     2701 gtggcacctg gcaggcgtac gggcaaggcc cgctgatctt caacgacccg gcctacggcc
     2761 aggtaaacgg cgaaggcatg gtcaatgtct ccggacgcgt cgatgcgttt gcctatgacc
     2821 cggccacgct gcgggtgttc gcgctgcccg gcaacggcgg cgtgtggacc tcgaccgact
     2881 tcgggcagaa ctggagttcg atcggagaca ggttgcctac gcagaacaac ggcgcggtgg
     2941 catggacctc ctccaacggc ggcacgctga tcgtgctgtc gggcaactcg gatttcctcg
     3001 gcagcccgtc gtacggcggc ctcggcgcgt tctggaccaa cgatcttggc cagacctgga
     3061 accagtcggt gggcataccc gacggcatct acgcatttga actggcggtg gaccacgccc
     3121 atcccgacat cgtctacgcc gccaccagcc gcggcctgtt ccgctccacc gatgccggcc
     3181 gccacttcgt caacgtggcg ctgcccacgt cgaccgattg cgccggccgt accgatctgg
     3241 gcaagtgcca gttcgccaac gtggtgacct cggtggtggt gcaggaaccg ggcggcacca
     3301 gcaatgttgc cggcggcgcg gtgctggccg cagtgggctt ccaggaaggc aacaccaaca
     3361 ccttctccga cggcacgctg gtgtcgccgg gcaatggcct gtatgcatcg cctgatggcg
     3421 cgcccggcag cttcaccaag ctggcggtgg gagcggccag cgcggtgtcg ccggtgggct
     3481 ttgcgccgga caaccgcatc ggccgcgtgg ccatggccgt ggcgcacggc gacgaccagg
     3541 accacaacta cgtctacgcg ctggtgcaag acgcgctgct catgaccggc ggcctgccct
     3601 acatcgacgt caatcccact tcgctcggcg cgccggtggc ggtgccgatt cccaccgtgc
     3661 tcaacggcct ctacatctcg tccgacttcg gccagagctg gacgcgcgca ttcgacttcg
     3721 tcgcctcgca gaatccggcg agcggcagtg cgctcgccgg cctgcagcag gccaccggct
     3781 acggccccgg cgtgcaggcc tggtacaacc tgtgggtcga atccgatccc acggtcaccg
     3841 ccagcaacgc gccttcgcgc ctgttcttcg gtcttgaaga ggtgtggtcg agcctcaccg
     3901 ccagcgtgcc gcagaacggc accgcgcagc tggccgacgt ggtgaactac cacgtgatcg
     3961 gcgcgtatta cgccggcacc acctgcggcg cgctcaacct gggcgccatt ttccccaacc
     4021 tgccgccggc ctgcccgggg cgcaatccgc ccaccgcgac caccaccacg caccccgacc
     4081 agcacgggca cctggtgatg cccgacggcc agggcggcgt gtacctgttc gccggcagcg
     4141 acggcggcgt gttcgtgcag caccacgcgg cgggcgagaa ctttgacaac gccggctggg
     4201 gcgacggcgc gcagaacggc ttcaacacgc tgctgccgta cgacattgcc gtggccaagg
     4261 acaacaccgt gtggttcggc ctgcaggaca acggcagcgg caagatcgac ccggtgacgc
     4321 agaaggtcta cgagacctac ggcggcgacg gcttctggat ggcggtcgac ccggacaaca
     4381 gcaacaccgc atacgaggag tacaccaacg gcgacatcag cgtgaccacc gacggcggcc
     4441 agacctggac cgacatgaac ccgaacctgg cgaaccccaa cttcgccaac cagttctaca
     4501 tggacccgac ggatgccaag cacatcctca ccggcggcag cgacatcgtc gacacggtgg
     4561 acggcccgga cacctgcgcc accaacacct gcacctgggc cacgctgttc acgctg
//
LOCUS       DASZZC010000787         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_763182,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000787 DASZZC010000000
VERSION     DASZZC010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763182"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VHE37_16955"
     CDS             <1..1376
                     /locus_tag="VHE37_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT37277.1"
                     /translation="RYKLAVLNVEYDLGWASAVSESAWVNKHSHTYLENTRNKVPNAP
                     VGLVAIDGGGDSDTYSQELRLVSADQPGDRWRWIAGVFASRQEIHEVVNLDAGNSTLP
                     AALNGGLLDSLIPGLGQEVDAMGNVSLIDLNPHVTIRELALFGNVTRKLGDAWELTLG
                     GRLYKTDSFGTNLQQGVVFTALNGSPQFVQHGGVAEHGFNPQLSLAWHATDELMSYAT
                     VAKGFRVGGLQPGITLPTSKTQAPPTFKSDTIWSYELGQRTSWFGRTLNVDVAAFHID
                     WKNPQVQQPDSTSGVAYLDNAGGVKSDGAELNLRWLTPVGLMLSSGMTYAKTVTTEPF
                     LSGTGQMSMPGDPWPYAPRLQTSTTLAYPLDLAGWVVTPSLTHAYLGKTVNNLGEQKP
                     IFNYHQLDAQIGLANPQWSWAPQLTISVNNVLDTRGVTTNVTQGPSTDVNYCQPRTLI
                     VHLGGQF"
     gene            complement(1509..2447)
                     /locus_tag="VHE37_16960"
     CDS             complement(1509..2447)
                     /locus_tag="VHE37_16960"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT37278.1"
                     /translation="MLDALRHLASFAGFAAQRTARERHDAAVQRELCARPLALPRGLE
                     LTWLGTAGYRLRYQGQSLYIDPYLSRVPLSALLRGQPALSDAQRVSAVVEPGEQVAGI
                     LVGHCHFDHAIDAPEIARRCHAQVYGSQSLARLMQLHGLHHLAVEVRAHQPYDIGPYK
                     VSFAPSAHSKLVLGHAVPYDGEFSCEHVDALSPAAYRCGQVWGIQVEVAGIRLYHQGS
                     ANLVDDEVRARNVDVFLAGIAGRRFTRDYWARILGRLQPRVVVASHYDDFFRPLDAPP
                     GFSANVNLSALPEEIGRVSREFSVVALQPLRPVTGA"
BASE COUNT          497 a          916 c          862 g          379 t
ORIGIN      
        1 accgctacaa gctcgcggtg ctcaacgtcg agtacgacct cggctgggcc agcgcggtgt
       61 ccgagtcggc gtgggtcaac aagcattcgc acacctacct ggagaacacg cgcaacaagg
      121 tgccgaacgc gccggtcggg ctggtggcca tcgacggcgg cggcgactcc gacacctaca
      181 gccaggaact gcgcctggtg tcggcggacc aacccggcga ccggtggcgc tggatcgccg
      241 gcgtgttcgc atcgcgccag gagatccacg aggtggtcaa tctcgacgcc ggaaattcca
      301 cgctgcccgc cgcgctcaac ggcggcctgc tggactcgct gattcccggg ctcgggcagg
      361 aagtcgacgc catgggcaac gtcagcctga tcgacctgaa tccgcacgtg accatccgcg
      421 agctggcgct gttcggcaac gtcacgcgca agctcggcga tgcgtgggag ctgacgctcg
      481 gcggccgcct gtacaagacc gattccttcg gcaccaacct gcaacagggc gtggtgttca
      541 ccgcgctcaa cggcagcccg cagttcgtgc agcacggcgg cgtggccgag catggcttca
      601 atccgcagct gtcgctggcc tggcacgcga ccgatgagct gatgagttac gccaccgtgg
      661 ccaagggctt ccgcgtcggc ggcctgcagc cgggcatcac cctgcccact tcaaaaacgc
      721 aggcgccgcc caccttcaag tccgacacca tctggagcta tgagctgggc cagcgcacct
      781 cgtggttcgg ccgcacgctg aatgtcgacg tcgccgcctt ccacatcgac tggaagaacc
      841 cgcaggtgca gcagcccgac agcacctcgg gcgtggccta cctggacaat gccggcggcg
      901 tgaagagcga cggcgccgaa ctgaacctcc gctggctcac gcccgtcggg ctcatgctca
      961 gctcgggcat gacctacgcg aagaccgtga ccaccgagcc cttcctcagc ggcaccgggc
     1021 agatgtcgat gccgggcgat ccgtggccgt acgcgccgcg gctgcagacc tcgaccacgc
     1081 tggcctatcc gctggatctc gccggctggg tggtcacgcc atcgctgacg cacgcctacc
     1141 tgggcaagac ggtgaacaac ctgggcgagc agaagccgat cttcaactac caccagctcg
     1201 acgcgcagat cgggctggcc aacccgcagt ggagctgggc gccgcagctc accatcagcg
     1261 tcaacaacgt gctggacacg cgcggcgtca ccaccaacgt gacgcaaggc ccatcgaccg
     1321 acgtgaacta ctgccagccg cgtacgctga tcgtgcacct gggcgggcag ttctgagcgc
     1381 ctccatccgc gcaaaaaaga cgtcaccgca ccccgccgga tgatgttata aattatttat
     1441 caatataaaa taagataaat atttagtaac acgccaacat gcggtgcggt gaaggcaaaa
     1501 aacgcgcttc aggcaccggt caccgggcgc aacggctgca gcgccacgac cgaaaactcg
     1561 cggctcacgc ggccgatctc ctccggcagc gcggacaggt tcacgttcgc ggaaaaaccc
     1621 ggcggcgcat cgagcgggcg gaagaaatcg tcgtagtggc tggcaacgac cacgcgcggc
     1681 tgcagccggc cgaggatgcg cgcccagtaa tcgcgggtga agcgccggcc ggcgatgccg
     1741 gcgaggaaca cgtcgacatt tctggcgcgc acttcatcat cgaccaggtt ggcgctgccc
     1801 tggtggtaca gcctgatgcc ggccacctcg acctggatgc cccacacctg cccgcagcgg
     1861 tacgcggcgg gcgagagcgc atcgacatgc tcgcaggaaa actcgccgtc gtacggcacc
     1921 gcatgtccca gcaccagctt gctgtgcgcg ctgggcgcga agctcacctt gtacgggccg
     1981 atgtcgtaag gctggtgcgc acgcacttcc accgccagat gatgcaggcc gtgcagctgc
     2041 atcagccgcg ccagcgactg cgagccgtac acctgggcat ggcagcggcg cgcgatctcc
     2101 ggcgcgtcga tggcatggtc gaagtggcaa tgcccgacca ggatgccggc cacctgctcg
     2161 ccgggttcca ccaccgccga cacgcgctgc gcatccgaca gcgcgggctg gccgcgcagc
     2221 agcgccgaca gcggcacgcg cgagagataa ggatcgatgt acaggctctg tccctggtag
     2281 cgcaggcggt agccggcagt gccgagccag gtcagttcca gcccgcgcgg cagcgccagc
     2341 gggcgcgcgc acagctcgcg ctgcacggcg gcgtcgtggc gctcgcgcgc ggtgcgctgc
     2401 gcagcgaatc cggcaaagct ggcgaggtgg cgcagggcgt cgagcaagcg tggactccgt
     2461 ggtgaacgta cgatatgcca tacgcccgtg atcgtcgcgt gaagcaaccc ccgccgcgcg
     2521 catggccggg cgcgcggttc actccatcgc cacggcgggc gcttcgtcgc ccttgcgcac
     2581 gcgcggcgtg cgcaccagca gcagcagcgg aatggtcagc agcgtgacca caaacatgaa
     2641 cttgaagtcg tcga
//
LOCUS       DASZZC010000788         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_638044,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000788 DASZZC010000000
VERSION     DASZZC010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638044"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VHE37_16965"
     CDS             complement(<1..1236)
                     /locus_tag="VHE37_16965"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HVT37279.1"
                     /translation="MRAALAAMLLWWWASSPALAQDAAPIGELPRTAEPLSYQLHFIL
                     DPQADGYTAEASIRVRLHQAADHLWLHGRSLTITQAQWTDASGTQGSAQYQQVNDDGV
                     ARVDFGRELAAQDLTLSFQFSGRYNRTLEGIYKVSRGGDDYLVTQLEAIAAREVFPCF
                     DQPSFKTPFDLSLTVPEKLVAVANSRQVGDERAAPGSKTLQFATTAPLPTYLLAFAVG
                     PWEVSELSHVPADAQRKHPLQLRVIGPRGSRALLDYALKTTPAIVAELENYFGEAYPF
                     DKLDLLAAPDFAFGAMENAGLIVYRESELLLDARASVETQQDYYLTNAHELAHQWFGN
                     DVTLRWWDDTWLNESFASWMEGKIVDRIHPEWHAGVSELQTARAAMEEDSLISARRIR
                     EPIVQTSDIDAAFDNITSEK"
     gene            complement(1304..1789)
                     /locus_tag="VHE37_16970"
     CDS             complement(1304..1789)
                     /locus_tag="VHE37_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT37280.1"
                     /translation="MQIENQRVVLMHYTLTNDAGEVLDSSRGGEPLAYLHGSGNIIPG
                     LENALAGKQAGDKVQVKVAPAEGYGERNPELVQQLPRRAFKGVGDLRVGMQLQAQSEH
                     GMRVITVTKIAGDMVTVDGNHALAGVPLNFDVEITEVRAATAEEMSHGHVHGPGGHHH
                     H"
     gene            2166..>2603
                     /locus_tag="VHE37_16975"
     CDS             2166..>2603
                     /locus_tag="VHE37_16975"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVT37281.1"
                     /translation="MYKRIGIIACLLAVIAGCSGHADEGKDKKEEAALPVEVAAVTSG
                     SIEAAYRGTATLEAEDEATVMAKTGGVIEQVLVEEGMRVSEGQVLARLETDKLKLQLE
                     QSKSSLDRLQQDFKRNESVYQRNLVSREAYDRTRYDLDGARAAY"
BASE COUNT          433 a          800 c          895 g          475 t
ORIGIN      
        1 tttctcagag gtgatgttat cgaaggcagc atcaatgtcg gaggtctgca caatcggttc
       61 gcggatgcgc cgcgcgctga tcaggctgtc ctcctccatc gcggcccgcg ccgtctgcag
      121 ctcgctcacg ccagcgtgcc attccgggtg gatgcggtcg acgatcttgc cttccatcca
      181 gctggcgaag gattcgttga gccaggtgtc gtcccaccag cgcagcgtca cgtcgttgcc
      241 gaaccactgg tgcgccagct catgtgcgtt ggtcaggtag tagtcctgct gggtctcgac
      301 ggatgcgcgc gcgtcgagca gcagttccga ctcgcggtag acgatcaggc cggcgttttc
      361 catggcgccg aaagcgaagt ccggcgccgc cagcaggtcg agcttgtcga acggatacgc
      421 ctcgccaaag tagttctcca gctccgccac gatcgccggc gtggtcttca gtgcgtaatc
      481 gagcagcgcg cggctgccgc gcgggccgat cacgcgcagc tgcagcggat gcttgcgctg
      541 cgcatcggct ggcacgtgcg acagttcgga tacttcccag gggccgacgg cgaaggccag
      601 caggtaggtc ggcagcggcg cggtggtggc gaactgcagg gtcttgctgc cgggcgcggc
      661 gcgctcgtcg ccgacctgcc gcgagttggc caccgccacc agcttttccg gcacggtgag
      721 cgaaagatcg aacggcgtct tgaacgaagg ctggtcgaag cacgggaaca cctcgcgcgc
      781 ggcgatggct tccagctgcg tgaccaggta atcgtcgccg ccgcggctga ccttgtagat
      841 gccttccagc gtgcggttgt agcggccgct gaactggaac gacagggtca ggtcctgtgc
      901 cgccaattcg cggccgaagt ccacgcgcgc cacgccgtcg tcgttgacct gctggtattg
      961 agcgctgccc tgcgtgccgg acgcgtcggt ccattgcgcc tgcgtgatgg tgaggctgcg
     1021 cccgtgcagc cacaggtgat cggcggcctg gtgcaggcgc acgcggatgc tggcctcggc
     1081 ggtgtagccg tcggcctgcg ggtccaggat gaaatgcagc tggtacgaca gcggctcggc
     1141 ggtgcgcgga agttcgccga tgggagccgc atcctgcgcc agcgccgggg acgacgccca
     1201 ccaccacagc aacatcgccg ccagcgcagc gcgcatgggc ctctccggga ttgtttagcc
     1261 gcgaggttac gcgcgcgccg cgcgagcggc aatcgagatg gcctcagtga tggtgatggc
     1321 cgccgggtcc gtgcacgtga ccgtgcgaca tctcctcggc ggtggcggcg cgcacttcgg
     1381 tgatctccac atcgaaattc agcggcacgc cggccagcgc gtggttgccg tcgacggtga
     1441 ccatgtcgcc ggcgatcttg gtcaccgtaa tcacgcgcat gccgtgctcg ctctgcgcct
     1501 gcagctgcat gcccacgcgc agatcgccca cgcccttgaa cgcgcggcgc ggcagctgct
     1561 gcaccagctc gggattgcgc tcgccatagc cttcggccgg cgccactttc acctgcacct
     1621 tgtcgccggc ctgcttgccg gccagcgcgt tttccagccc ggggatgatg ttgccggagc
     1681 cgtgcagata ggccagcggc tcgccgccgc gcgagctgtc gagcacttcg ccggcgtcgt
     1741 tggtgagcgt gtaatgcatc agcacgacac gttggttctc gatctgcatg gaaagggctc
     1801 ggagaaaacg ggccgcggca gggcggcaaa agggcggcga ttgtagtggg gcatgaaacc
     1861 ttttgcctgt ttcgagcatc tactgcgcgc cgtgcactgc caccgcaagg cggtggtgct
     1921 tgttataaaa tattgttcga ttatttattc gatatatatt ttgtaacatc aggatggccg
     1981 gtgcggttgg caggttttgc gggacctgcg tggcgcatat gccgaacagt tggggaattt
     2041 gaccgttcgg cacgagtgca agtttgcgcg tgaccgaaac tggtctttcg catcagtgag
     2101 ttgcgagggc ggcaaactgg catagcattt ggtttggagc ggggcgttcc atctaaggga
     2161 tcaacatgta caagcgcata ggaatcatcg cgtgcctgct cgccgtcatc gcgggctgct
     2221 ccgggcacgc tgatgaaggc aaggacaaga aagaagaagc cgcgctgccg gtggaagtgg
     2281 cggcggtgac ttccggttcc atcgaggccg cgtatcgcgg gaccgccacg ctcgaagccg
     2341 aggacgaggc cacggtgatg gccaagaccg gcggcgtgat cgagcaggtg ctggttgagg
     2401 aaggcatgcg cgtgagcgag ggccaggtgc tggcgcggct ggagaccgac aagctcaagc
     2461 tgcagctgga gcagtccaag tccagcctcg accgtttgca acaggacttc aagcgcaacg
     2521 agtcggtgta ccagcgcaac ctggtgtcgc gcgaagcgta cgaccgcacg cgctacgacc
     2581 tcgacggcgc gcgcgccgcc tac
//
LOCUS       DASZZC010000789         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_704182,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000789 DASZZC010000000
VERSION     DASZZC010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704182"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VHE37_16980"
     CDS             <1..845
                     /locus_tag="VHE37_16980"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HVT37282.1"
                     /translation="KLCEMWSPQGVGALGQVNQALSHGEAAEIPEMLAPLLDLARRVQ
                     QASDGAFDVRTGRLLRLWGFSQGSARRDQPPSELELAPLLSDLRAARPLGPGKYGPAA
                     VQLDFGGVAPGYIADLAASYLEQNGVHDAIITLGDNIKAIGTRGGKPWKVGIRHPHAD
                     AAQSMLASIELQGGEAVLTSGDYEQFFEYQGHRYHSILNPHTGEPAQGLQSVTVVGPS
                     AAWAQAASNALFVAGPQNWRSMARTLGVTQALVVDEGGSLSATAALVARLQFSAGVQA
                     TSVP"
     gene            912..1475
                     /locus_tag="VHE37_16985"
     CDS             912..1475
                     /locus_tag="VHE37_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HVT37283.1"
                     /translation="MKKDESNFKNQFLIAMPGLEDSNFDHAVTLMCEHSGEGAIGLVI
                     NRPTDLKLSEMLAQMGLEHDALPADISVFWGGPVAPERGFVIHRAPGGWESCLQLQRD
                     LYITTSRDILKAIGKGEGPKDYFVVLGYAGWGEGQLETEILGNSWLSAPASGQILFHT
                     PARDRWKEATRLLGVDVTQLSNDVGHA"
     gene            1486..>1710
                     /gene="ruvX"
                     /locus_tag="VHE37_16990"
     CDS             1486..>1710
                     /gene="ruvX"
                     /locus_tag="VHE37_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HVT37284.1"
                     /translation="MIFLAFDYGEKRIGAAVGDDVTGSARPLPVLASGEWKSIERVIA
                     EWRPAEIIVGLPLDDSGAEQPMTVAARRFAA"
BASE COUNT          284 a          584 c          582 g          260 t
ORIGIN      
        1 gcaagctgtg cgagatgtgg tcgccgcagg gcgtcggcgc gctgggccag gtcaaccagg
       61 cgctgtcgca cggcgaagcg gcggagatcc cggaaatgct ggcgccgttg ctcgatctgg
      121 ccaggcgcgt ccagcaggcc agcgacggcg ccttcgacgt gcgcaccgga cgcctgctgc
      181 ggctgtgggg tttcagccag ggcagcgcac ggcgcgacca gccgccgagt gaattggaac
      241 tcgcgccgct gctgtcggac ctgcgcgccg cgcgcccgct cgggccgggc aagtacgggc
      301 cggccgcggt gcagctggat ttcggcggcg tcgctcccgg ctacattgcg gatcttgccg
      361 ccagctatct cgaacagaac ggcgtgcacg acgccatcat cacgctgggc gacaacatca
      421 aggccatcgg cacgcgcggc ggcaagccgt ggaaagtcgg catccgccat ccacacgcag
      481 atgccgcgca gagcatgctg gcgtccattg aactgcaggg cggcgaagcg gtgttgacca
      541 gcggcgacta cgagcaattc tttgaatacc agggccaccg ctaccattcc atcctcaacc
      601 cgcacacagg cgaacctgcg cagggcctgc agtcggtgac ggtggttgga ccgagcgccg
      661 cctgggcgca agccgcgagc aacgcgctgt tcgtggccgg cccgcagaac tggcgcagca
      721 tggcgcgtac gctgggcgtc acgcaggcgc tggtggtcga cgagggcggc tcgctcagcg
      781 ccaccgcagc cctggtggca cggctgcaat tcagcgccgg cgtccaggcc accagcgtcc
      841 cctgaaccgc ccgtcagcgc cgcccggcgg cctttcttgc acgcgccgga cggcagccgg
      901 atactgggtg catgaagaag gacgagtcga acttcaagaa ccagttcctc atcgccatgc
      961 cggggctgga agacagcaac ttcgaccatg ccgtgacgct gatgtgcgag cacagcggcg
     1021 aaggcgccat tggcctggtg atcaaccgcc ccaccgatct caagctgtcc gagatgctgg
     1081 cgcagatggg cctggagcac gacgcgctgc cggccgacat ctcggtgttc tggggcgggc
     1141 cggtggcgcc ggagcgcggc ttcgtcatcc accgcgcgcc gggcggctgg gagtcctgcc
     1201 tgcagctgca gcgcgacctt tacatcacca cctcgcgcga catcctcaag gccatcggca
     1261 agggcgaagg cccgaaggat tatttcgtcg tgctgggcta cgccggctgg ggtgaaggcc
     1321 agctggaaac ggaaattctc ggcaactcct ggctcagcgc gccggcctcc ggccagatcc
     1381 tgttccatac gcctgcgcgc gaccgctgga aagaagccac gcgcctgctc ggcgtcgacg
     1441 tcacccagct ttccaacgac gtcggccacg cctgattttt ccggcgtgat ttttctcgcg
     1501 ttcgactacg gcgaaaagcg catcggcgct gctgtcggcg acgacgtcac cggcagcgcg
     1561 cgcccgctgc cggtgctcgc cagcggagaa tggaaatcga tcgagcgcgt catcgctgaa
     1621 tggcgcccgg cggaaatcat tgtcgggctg ccgctggatg attccggcgc cgagcagccg
     1681 atgaccgtcg ctgcgcgccg cttcgccgcc
//
LOCUS       DASZZC010000790         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_126643,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000790 DASZZC010000000
VERSION     DASZZC010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126643"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VHE37_16995"
     CDS             complement(<1..334)
                     /locus_tag="VHE37_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HVT37285.1"
                     /translation="MERLSDLARRLGIAAPAVDARFERVVKDTRTLQAGDLYVALVGE
                     NFDGHQFVAAARAAGAAAALVSRAVDDPLPQLVVPDTLQALQQYAASWRADFDIPVVA
                     VTGSNGKTT"
     gene            complement(322..1839)
                     /locus_tag="VHE37_17000"
     CDS             complement(322..1839)
                     /locus_tag="VHE37_17000"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HVT37286.1"
                     /translation="MSDSVSPRSLRKLLAGFGAPAPDVEVSGIEMDSRRVSPGDLFLA
                     CKGGGTHGLAHLEQALERGAAAVAWEPAQGWPQPEIGVPEIAIEQLSSRAGRIAARYY
                     GEPSQRMYVCGVTGTDGKTSTAHLIAQALDRLGTRCAYVGTLGSGYVDALAKGARTTP
                     DPVSLQKQLAHFVEMGAQATAMEVSSHALHQERTAGMKFDTAILTNVTRDHLDYHGTV
                     ENYAAAKRKLFEREELSALVLNRDDATGARWLQEFSERPVIAYGIDGDVPKAGRHVIV
                     RKLRAHAGGLSFDIDSSWGALHIESRLLGRFNAYNLSAALAAILHRGLVTEKVAAALA
                     QASTVPGRIEGFRGPAARPLVVVDYAHTPEALAQVLTAVRAHTAKKLWCVFGCGGDRD
                     RGKRPLMGAAAARHADQLIVTDDNPRSEKPAAITAEILAGLSRGTAHRVIHDRAEAIR
                     TAVSEAQADDVVVVAGKGHEAEQIYGTEVRPFSDRHFVAGLVGAPTPGGGARWNA"
     gene            complement(1836..3566)
                     /locus_tag="VHE37_17005"
     CDS             complement(1836..3566)
                     /locus_tag="VHE37_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVT37287.1"
                     /translation="MRAIPNLSVQPVEVWRRWLVLGVLTLGVAAVFGRAFQLQVLQRD
                     FLVREGDRRHIRTVDIPGHRGAILDRRGEPLALSAPVESIWAVPEALLESPQHVYAMA
                     RLLEKNPREFQKFLQDRVDRKFVYLTDPIDPAAAQRIMALKAPGVFSESDYKRYYPAG
                     EVTAQIVGFCGRDGNGLEGVEHAQQKALAGAAGSMRVIRDRTGRIVEDGLEAVEARPG
                     QDLRLTLDLRLQYLAYRELKAAVAKNNARGGLIVVADSRNGDILAMASQPGYNPNNMD
                     ERTSRGMRNRAIVDLFEPGSTVKPLLVAQALELGAYKPDSHVDTTPGWLKVGALTVRD
                     VHPQGDIDLAHVLSRSSNVGAAKIGLSMGPEAVWNGFERFGLGDPVYSGFPGEATPVL
                     RPYAEWGQIATATASYGYGLSLTALHLVRAYTALANDGLMSQLRLLDGAPHVPPQRAI
                     SPTVARELRHLLEGVTEAGGTATAAAIPGYRVSGKTGTIRKTVEGGGYAKDSHQAVFI
                     GMLPAENPRLVGMIMIDDPTAGDYYGGLVSAPVFSRVMQGAARLLQIPPDRATLQPDE
                     QLVAAAGGQR"
     gene            complement(3563..3847)
                     /gene="ftsL"
                     /locus_tag="VHE37_17010"
     CDS             complement(3563..3847)
                     /gene="ftsL"
                     /locus_tag="VHE37_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HVT37288.1"
                     /translation="MRTSMRDLFLFLLVAGVIGSALAVVRTKHENRALVSQLESLRLE
                     HERLEMEWAQLQLEEATLAHNNRVDKIAREQLQMSDPRDYRIVTAGAPAR"
     gene            complement(3847..>3998)
                     /gene="mraW"
                     /locus_tag="VHE37_17015"
     CDS             complement(3847..>3998)
                     /gene="mraW"
                     /locus_tag="VHE37_17015"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HVT37289.1"
                     /translation="DRMVKRYLRDQARETLKLSVHKKQFPSDEECAANPRARSAVLRV
                     AEKLA"
BASE COUNT          595 a         1400 c         1355 g          648 t
ORIGIN      
        1 tcgtggtctt gccgttgctg ccggtgacgg cgacgaccgg gatgtcgaaa tccgcgcgcc
       61 acgacgcagc gtactgctgc agggcctgca gagtgtccgg caccaccagc tgcggcagcg
      121 gatcgtccac cgcgcgcgac accagcgccg cagccgcgcc ggccgcgcgc gcggcggcga
      181 cgaactgatg gccgtcgaaa ttctcgccga ccagcgcgac gtagagatcg cccgcctgca
      241 gcgtgcgcgt gtccttcact acgcgctcga accgcgcgtc gaccgctggc gcggcaatac
      301 cgaggcggcg cgcgagatcg ctcaggcgtt ccatcgtgca cctccgccgg gggtcggtgc
      361 gcccaccagt ccggcgacga agtgccggtc cgaaaacggc cgcacctccg tgccgtagat
      421 ctgctcggcc tcgtggccct tgccggccac caccaccacg tcatcggcct gcgcttcgct
      481 cactgctgta cggatggctt cggcgcggtc gtggatcacg cggtgcgcag ttcccctgga
      541 caggcccgca aggatttccg cggtgatggc cgccggcttc tcgctgcgcg ggttgtcgtc
      601 ggtgacgatc agctgatcgg cgtggcgcgc cgccgccgcg cccatcagcg ggcgcttgcc
      661 gcggtcgcgg tcgccgccgc agccgaacac gcaccagagt tttttcgcgg tatgcgcgcg
      721 caccgcggtg agcacctgcg ccagcgcctc gggcgtgtgc gcgtagtcca ccaccaccag
      781 cgggcgcgcg gcggggccac ggaaaccttc gatgcggccg ggcacggtcg acgcctgtgc
      841 cagcgccgct gctacttttt ccgtaacaag gccgcggtgc aggatggcgg ccagcgcggc
      901 tgacaggttg taggcattga agcggccgag cagccggctc tcgatatgca gtgcgcccca
      961 gctgctgtcg atatcgaacg aaaggccgcc ggcatgtgcg cgcagcttgc gcacgatgac
     1021 gtgacggccg gccttcggca catcgccgtc gatgccgtag gcgatcaccg ggcgctcgga
     1081 gaattcctgc agccagcgcg cgccggttgc gtcatcgcgg ttgagcacca gcgccgagag
     1141 ttcttcccgc tcgaacagct tgcgcttggc cgcggcgtag ttctcgacgg tgccgtggta
     1201 gtccaggtga tcgcgggtga cgttggtgag gatggccgtg tcgaacttca tgccggcggt
     1261 gcgttcctga tgcagcgcat gcgaggacac ttccatggcc gtggcctgcg cgcccatctc
     1321 gacgaaatgc gccagctgct tctgcaggct gaccggatcg ggcgtggtgc gcgcaccctt
     1381 ggccagcgca tcgacataac ccgagcccag cgtgcccacg taggcgcagc gcgtgcccag
     1441 gcgatcgagc gcctgcgcga tcaggtgcgc ggtcgacgtc ttgccgtcgg tgccggtgac
     1501 accgcacacg tacatgcgct gcgaaggttc gccgtagtag cgcgccgcga tgcggccagc
     1561 acgcgacgaa agttgctcga tggcgatttc cggcacgccg atttcaggct gcggccagcc
     1621 ctgcgcaggt tcccaggcca ccgcggcagc gccgcgctcg agcgcctgct ccagatgcgc
     1681 gagcccatgc gtcccaccgc ccttgcaggc caggaacagg tcgccgggcg acacgcgccg
     1741 gctgtccatc tcgatgccgc tgacttccac gtccggcgcc ggcgcgccga aaccggccag
     1801 cagtttgcgc aaggaacgcg gcgagacgct gtcgctcatc gctgccctcc tgcggccgcg
     1861 accagctgtt cgtcgggctg cagcgtggcg cggtcaggcg gaatctgcag caggcgcgcc
     1921 gcgccctgca tcacgcgcga gaacaccggc gcggatacca acccgccgta gtagtcgccg
     1981 gccgtggggt cgtcgatcat gatcatgccc accaggcgcg gattttccgc cggcagcatg
     2041 ccgatgaata ccgcctggtg gctgtccttg gcgtagccgc cgccttcgac cgtcttgcga
     2101 atggtgccgg tcttgccgct gacgcggtag ccgggaatcg ccgccgcggt cgcggtaccg
     2161 ccggcttcgg tcacgccttc gaggaggtgg cgcaattcac gcgccaccgt gggtgaaatc
     2221 gcgcgctgcg gcggaacgtg cggcgcacca tcgagcagac gcagctgcga catcaggccg
     2281 tcgttggcca gcgccgtgta cgcgcggacc aggtgcagcg cggtgagcga gagtccgtag
     2341 ccgtatgaag ccgtcgccgt ggcaatctgg ccccactcgg cgtacggccg cagcaccggc
     2401 gtggcttcac cgggaaaacc cgagtagacc ggatcgccca ggccgaagcg ctcaaagccg
     2461 ttccatacgg cctccggtcc catcgacaaa ccgatcttgg ccgcgccgac gttcgacgag
     2521 cgcgacagca cgtgtgcgag atcgatatcg ccctgcgggt gcacgtcgcg cacggtcagc
     2581 gcgccaacct ttaaccagcc cggcgtggta tccacgtggc tgtccggctt gtacgcgccg
     2641 agctcgagcg cctgcgccac cagcagcggc ttgaccgtgg agccaggttc gaacaggtcg
     2701 acgatggcgc ggttgcgcat gccgcgcgag gtgcgctcgt ccatgttgtt ggggttgtaa
     2761 ccgggctgcg acgccatcgc caggatgtcg ccgttgcggc tgtccgccac cacgatcagc
     2821 ccgccgcgcg cgttgttctt ggccaccgcg gccttcagct cgcggtaggc cagatattgc
     2881 aggcgcaggt cgagcgtcag gcgcaggtcc tggcctggcc gcgcttcgac ggcttcaagg
     2941 ccgtcctcga cgatgcgccc agtgcggtcg cggatcacgc gcatcgaccc cgcggcgccg
     3001 gccagcgcct tctgttgcgc gtgttccacg ccttccaggc cgttgccgtc gcgcccgcag
     3061 aaaccgacga tctgcgcggt gacttcgccc gccgggtagt agcgcttgta gtcgctctcg
     3121 gaaaacacgc ccggcgcctt cagcgccatg atgcgctgcg cggcggccgg atcgatggga
     3181 tcggtgaggt agacgaactt gcggtcgaca cgatcctgca ggaatttctg gaactcgcgc
     3241 ggattcttct ccagcaggcg cgccatggca tagacgtgct gcggcgattc gagcagcgcc
     3301 tcgggcaccg cccagatcga ttccaccggc gccgacaagg cgagcggctc accgcggcga
     3361 tcgaggatgg ctccgcggtg tccggggatg tcgaccgtgc ggatgtggcg gcgatcgcct
     3421 tcgcgcacca ggaagtcacg ctgcagcacc tgcagctgga aagcacggcc gaagaccgcg
     3481 gccacgccga gcgtcagcac gcccagcacc agccagcggc gccacacttc caccggctgc
     3541 accgacaggt tgggaatggc cctcatcgcg ccggcgctcc cgcggtgacg atgcggtaat
     3601 cgcgcgggtc cgacatctgc agctgctcgc gcgcgatctt gtcgacgcgg ttgttgtgcg
     3661 ccagcgtcgc ttcttccagc tgcagctgcg cccattccat ctccaggcgc tcgtgctcga
     3721 ggcgcagcga ttccagctgc gacaccagcg cccggttttc gtgcttggtc cgcaccaccg
     3781 ccagggcgga gccgatgacg ccggccacca gcaggaacag gaacaggtcg cgcatgctgg
     3841 tgcgcatcag gcgagcttct cggccacgcg cagcaccgcg ctgcgtgcgc gcggattggc
     3901 cgcgcattct tcatccgagg gaaactgctt cttgtgcacc gacagtttca atgtctcgcg
     3961 cgcctgatcg cgcaggtagc gtttgaccat gcggtctt
//
LOCUS       DASZZC010000791         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_126661,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000791 DASZZC010000000
VERSION     DASZZC010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126661"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VHE37_17020"
     CDS             complement(<1..1607)
                     /locus_tag="VHE37_17020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT37290.1"
                     /translation="MILCACCGTARAQDAAEPSAADTAAPAQGEVATIPVAEAAPAAE
                     TTPASSEAGAPELGEVVVTAQKTKQNARSVPISMSVLSDRFIAQHGIVDIVQAMQYVP
                     NVKIASAGFFAAPQARGFSFNNNNKAFESPLGIAFDGIPYTNTTYFNAGLFDIERVEV
                     LRGPQGTTFGKNTTAGLVSIVTADPTPDWVGLVSVQGGEIGRQHYEAALSGPLIEDVV
                     DFRIAAIKDELGGYVRNTTAAVVPTADPVFRGYAHNGYRAKLKFPDLWGTQLKLSFEA
                     VDMSSLGAGIEVWDATEPVKTVMKRYDPNADFVLGNLVNSEDQPENRLARTKTAVIEW
                     TVPAGDWELTALGGWSQLKETVGIDVDFSPVPGSTGDGGDRFPTTTLELRAKSPTLDG
                     LFGLHSLLGFDLGHTDFLGGFFMQRRTIRDSDLRLGLNDGPLLELTAAAELPDTGTST
                     DQLNTYAYLLGSLGLNAAEASQGHEVFDQVYDQEGEVLALFQQTEWAFLPDWKLQLGL
                     RASRETKKGYWNDYFEGADDVVGTALGL"
     gene            complement(1705..2370)
                     /locus_tag="VHE37_17025"
     CDS             complement(1705..2370)
                     /locus_tag="VHE37_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT37291.1"
                     /translation="MKKAESYRRLASEQMLLPADGTPARGSRSGILSAALKLFAERGY
                     GGTSVRDIAAVSQVQPATLYAHFPSKQHVLSELIALGHEEHLKRMRAALLETSADPAE
                     QIAALVRAHVEFHTTYPMLSVVANSELHSLEGGFAAPALALRKQAEQMLSEVIRRGIE
                     KGEFDVPHEWLAMAWIGGAGLRVGNWYTEDFELPPEAVAEHYVELAWRVLGVTRKRTG
                     RKK"
     gene            2482..>2843
                     /locus_tag="VHE37_17030"
     CDS             2482..>2843
                     /locus_tag="VHE37_17030"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT37292.1"
                     /translation="MSLPYRHIDLPRAGATLRAGIWGNGPRIVLASHGITANHVTFKP
                     IVDRLDAGDTLIAPDHRGRARSNGIAGPWGMQEHAEDLIALLDHLHIDRVDVLLGQSM
                     GGFVSAVAAARHPQRFGAL"
BASE COUNT          454 a          969 c          909 g          511 t
ORIGIN      
        1 agcccgagcg ccgtgccgac cacatcgtcc gctccttcga aatagtcgtt ccagtagccc
       61 ttcttggttt ccctgctggc gcgcaggccc agctgcagtt tccagtcagg caggaaggcc
      121 cactcggtct gctggaacag cgccagcact tcaccttcct ggtcgtacac ctggtcgaac
      181 acttcgtgtc cctgcgacgc ctcggcggcg ttgagcccga gcgagccgag caggtaggcg
      241 taggtgttga gctgatcggt gctggtgccg gtgtccggca gttcggcggc ggcggtcagt
      301 tcgagcagcg ggccgtcgtt gagacccagg cgcagatcgc tgtcgcggat ggtgcgccgc
      361 tgcatgaaga agccgccaag aaagtcggta tgcccgagat cgaaacccag caggctgtgc
      421 aggccgaaca gcccgtccag ggtcggcgac ttggcgcgca gttccagcgt ggtggtggga
      481 aagcggtcac cgccatcgcc ggtgctgccg ggcaccggag agaaatcgac gtcgatgccc
      541 accgtctcct tgagctgcga ccagccgccc agcgcggtga gttcccagtc gccggccggc
      601 accgtccact cgatcaccgc cgtcttggtg cgcgccaggc gattctccgg ctgatcctcg
      661 gaattgacca ggttgccgag cacgaaatcg gcattcgggt cataccgctt catcaccgtc
      721 ttgaccggct cggtggcgtc ccacacctcg atgccggcgc cgagactgga catgtcgacc
      781 gcctcgaagc tcagcttgag ctgcgtgccc cacaggtctg gaaatttcag cttggcgcgg
      841 tagccattgt gcgcatagcc gcggaacacg ggatcggcgg taggtaccac cgcggcggtg
      901 gtgttgcgca cgtagccgcc cagttcgtcc ttgatcgcgg cgatgcggaa atccaccacg
      961 tcttcgatca gcggcccgct cagcgcggct tcgtaatgct gccgtccgat ctcgccgccc
     1021 tgcaccgaaa ccaggccgac ccagtccggc gtgggatccg cggtgacgat gctgaccagt
     1081 ccggcggtgg tgttcttgcc gaaggtggtg ccctgcggac cacgcagcac ttccacgcgc
     1141 tcgatgtcga acagcccggc gttgaagtac gtggtgttgg tgtacggaat gccgtcgaag
     1201 gcgatgccca gtggcgactc gaaggccttg ttgttgttgt tgaacgagaa gccgcgcgcc
     1261 tgcggtgcgg cgaagaatcc ggcggaagcg atcttcacgt tcggcacgta ctgcatggcc
     1321 tgcacgatgt cgacgatgcc atgctgggcg atgaacctgt cggacagcac cgacatggag
     1381 atcggcacgc tgcgcgcgtt ctgcttggtc ttctgcgcag taacgaccac ctcgcccagt
     1441 tccggcgcgc cggcctcgct ggaagcgggt gtcgtttccg cggccggcgc cgcttctgcg
     1501 acgggaatcg tcgcgacctc gccttgcgcc ggtgctgcag tgtccgcagc ggacggctca
     1561 gccgcatcct gtgcccgcgc tgtgccgcag caggcgcaca ggatcaccga tgcaaaccac
     1621 cgcgcgtacc tgcccgctcc cgcccgaggc tgtccccgca tgcgccgtct cctctcgctc
     1681 gccgccgcac cgcagcagct atttctattt ctttctgccc gtgcgcttgc gcgtcacccc
     1741 gagcacgcgc caggcaagct ccacgtagtg ttcggccact gcttccggcg gcagctcgaa
     1801 atcctcggtg taccagttgc ccacgcgcag gccggcgccg ccgatccagg ccatcgccag
     1861 ccactcgtgg ggaacgtcga actctccttt ctcgatgccg cgccggatca cctcgctcag
     1921 catctgctcc gcctgcttgc gcaaggccag cgccggcgca gcgaagccgc cttccagcga
     1981 atgcagttcc gaattggcca ctaccgacag catgggatag gtggtgtgga actcgacgtg
     2041 cgcgcgcacc agcgccgcga tctgctccgc cggatcggcc gaggtttcga gcagtgcggc
     2101 gcgcatgcgc ttcaggtgct cctcgtggcc gagcgcaatc agttccgaca gcacatgctg
     2161 cttcgaggga aagtgcgcat agagcgtggc cggctgcacc tgcgacaccg cggcgatgtc
     2221 gcgcaccgag gtgccgccat agccgcgctc ggcaaagagc ttgagcgcag cggacaggat
     2281 gccgctgcgc gagccccgcg caggcgtgcc atcggccggc agcagcatct gctctgaggc
     2341 caggcggcgg tagctctcgg cttttttcat gaaccgaacg atcgttcgta gaaatggcgt
     2401 aagcagatgc tatgcatgaa tatttttgtt gtcaaccagc gtacgttcgt tctagtattg
     2461 atccacgatg gagatcaacg catgagcctg ccctatcggc acatcgacct gccgcgcgcc
     2521 ggcgccacgc tgcgcgccgg catctggggc aacggcccgc gcatcgttct ggcctcgcac
     2581 ggcattaccg ccaatcacgt gacgttcaag cccatcgtcg atcgcctcga cgccggcgac
     2641 acgctgatcg cgcccgacca ccgcggccgc gcgcgcagca acggcattgc cggcccgtgg
     2701 ggcatgcagg agcacgcgga agacctgatc gccctgctcg atcacctgca catcgaccgc
     2761 gtcgacgtgc tgctcggcca gtcgatgggg ggcttcgtct cggcggtggc cgcggcacgt
     2821 catccgcagc gcttcggtgc gct
//
LOCUS       DASZZC010000792         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_704307,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000792 DASZZC010000000
VERSION     DASZZC010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704307"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /gene="nuoG"
                     /locus_tag="VHE37_17035"
     CDS             complement(<1..2012)
                     /gene="nuoG"
                     /locus_tag="VHE37_17035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HVT37293.1"
                     /translation="MKQFKDESDKMGRIVMSCMTPAANNSYISIGDAEAKDFRKSVIE
                     FLMTNHPHDCPVCEEGGHCHLQDVTVMTGHHSRAYRFDKRTHNNQYLGPFVAHEMNRC
                     ISCYRCVRYYNEYAGGTDFGVFGAAANVYFGRAEEGVLENEFSGNLTEVCPTGVFTDK
                     THSDRYVRKWDMQFAPSVCSGCAVGCNVSPGERYGEIRRIENRYHGELNGYFLCDRGR
                     FGYGYTNRKDRPRQALQRRAGELVAVDAQGATAQARALLKEARRIVGIGSTRASLESN
                     YALRVLVGDSNFSSGISAREAELVRLATQIIGSGAAPIATIRGMEEADAVLVLGEDVT
                     QTAPRVALALRQASRGKMTSEGAKKNVPDWQVIALADLVQNAKNPLFVATPDATRLDD
                     IATQTYRAAPADLARLGLAVANLLDAAAPAVRGLDPATLKLAQQIAAVLKAAKKPLIV
                     SGCGAGSAMVMQAAANIARALKHGGSPVQLSLALPDMNSFGLALIESQDAETALASVG
                     EGDALVVLENDLYRHAPRALADAALARAKLVIVVDHQRTATTDKAHLVLPAASIFEGD
                     GTAVNFEGRAQRYFQVFDASYYKKDNQVLESWRWLDALGAGQWRNLDAVTAACAQALP
                     QLAAIVDAAPNASFRLKGLKIAREPHRYSGRTAMRANISVHEPRAP"
BASE COUNT          318 a          681 c          678 g          365 t
ORIGIN      
        1 tgcggcgcgc gcggttcatg cacgctgatg ttggcgcgca tcgcagtgcg cccgctgtag
       61 cggtgcggct cgcgcgcgat cttcagtccc ttgaggcgga agcttgcgtt gggcgcggca
      121 tcgacgatgg ccgccagctg cggcaacgcc tgcgcgcagg cggcggtgac ggcgtcgaga
      181 ttgcgccact gcccggcgcc gagcgcatcc agccagcgcc agctctccag cacctggttg
      241 tccttcttgt agtacgaagc gtcgaacacc tggaagtaac gctgggcgcg cccttcgaaa
      301 ttaaccgcgg tgccatcgcc ttcgaagatg cttgccgccg gcagcaccag atgcgccttg
      361 tcggttgtcg cggtgcgctg gtgatcgacc acgatcacca gcttggcgcg cgccagcgcg
      421 gcgtccgcca gtgcgcgcgg cgcatggcga tacaggtcat tctccagcac taccagtgcg
      481 tcgccctcgc ccaccgacgc cagcgcggtc tcggcatcct gcgattcgat cagcgccagg
      541 ccgaagctgt tcatgtcggg caaggccagc gacagctgca ccgggctgcc gccgtgcttg
      601 agcgcgcgcg cgatgttggc tgccgcctgc atcaccatcg cagaacctgc gccgcagccg
      661 gagacgatca gcggcttctt cgccgccttc agcaccgcgg cgatctgctg cgccagcttg
      721 agcgtggccg ggtcgagccc gcgcaccgcc ggcgccgcgg catcgagcag gttggcgacc
      781 gccaggccaa gccgcgccag gtcggctggc gctgcacggt aagtctgcgt ggcgatgtcg
      841 tcgagacgcg tcgcatcggg cgtggcgacg aacagagggt tcttcgcgtt ctgtaccaga
      901 tcggccagcg caatgacctg ccagtcgggc acgttcttct tggcgccttc actggtcatc
      961 ttgccgcgcg acgcctggcg cagcgccaga gccacgcgcg gcgcggtctg cgtcacgtcc
     1021 tcgccgagca ccagcaccgc atcggcctct tccatgccgc ggatggtggc aatgggcgca
     1081 gcgccgctgc cgatgatctg cgtggccagg cggaccaatt cggcttcgcg cgcagatatg
     1141 ccgctggaga aattgctgtc acctacgagc acgcgcaacg cgtagttgga ttccagcgat
     1201 gcgcgggtcg atccgatccc gacgatgcgg cgggcttcct tgagcagggc acgggcctgt
     1261 gcggtcgctc cctgcgcatc gaccgcgacc agttcgccag cgcggcgctg cagtgcctgg
     1321 cgcgggcgat ccttgcggtt ggtgtagccg tacccgaagc gcccgcggtc gcagagaaaa
     1381 tagccattga gctcgccgtg gtaacggttc tcgatgcggc gaatctcgcc gtagcgctcg
     1441 cccggcgaga cgttgcagcc caccgcgcag cccgaacaca ccgatggcgc gaactgcatg
     1501 tcccacttgc gcacgtaacg atccgagtgc gtcttgtcgg tgaacacgcc ggtcgggcaa
     1561 acctcggtaa ggttgccgga gaactcgttt tcgagcacgc cttcttcagc gcggccgaag
     1621 tagacgttgg ccgcagcgcc gaacacgccg aaatcggtgc cgccagcgta ctcgttgtag
     1681 tagcgcacgc agcggtagca cgagatgcag cggttcatct cgtgtgccac gaacgggccc
     1741 aggtactggt tgttgtgagt gcgcttgtcg aagcggtagg cgcgcgagtg atggccggtc
     1801 atcaccgtca cgtcctgcag gtggcagtgg ccgccttcct cgcacaccgg gcaatcatgc
     1861 ggatgattgg tcatcaggaa ttcaatgacc gatttgcgga aatccttggc ctcggcgtcg
     1921 ccgatcgaga tgtagctgtt gttggccgcc ggtgtcatgc acgacatgac gatgcggccc
     1981 atcttgtcgc tctcatcctt gaattgcttc accgcgcact ggcggcaggc gccgacgctg
     2041 cc
//
LOCUS       DASZZC010000793         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_127118,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000793 DASZZC010000000
VERSION     DASZZC010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127118"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /gene="clpA"
                     /locus_tag="VHE37_17040"
     CDS             <1..296
                     /gene="clpA"
                     /locus_tag="VHE37_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648418.1"
                     /note="ATPase and specificity subunit of the ClpA-ClpP ATP
                     dependent serine protease; directs protease to specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HVT37294.1"
                     /translation="RVVDKFLMQLEEQLGAKHVQLDVDEEAKDWIALRGYDVKMGARP
                     MARVIQENLKRPLAEELLFGKLANGGKVTVTVKDDKLEFEMETRDKPVTLEPV"
     gene            complement(355..792)
                     /locus_tag="VHE37_17045"
     CDS             complement(355..792)
                     /locus_tag="VHE37_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT37295.1"
                     /translation="MDINGIAHVILTTGDFARAREFYGKLLPLLGMKQVLDQPGYFYC
                     VGGRTALGIKVADPEYRGQRFVQARVGLQHLCFRARERADIDALHAWLQQQGATIVHG
                     PQEDRWAPGYYSVLFEDPDGIRLEMNHVPGKGLLEVDRKFGAP"
     gene            complement(932..>3047)
                     /locus_tag="VHE37_17050"
     CDS             complement(932..>3047)
                     /locus_tag="VHE37_17050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT37296.1"
                     /translation="GTLALAGDDGNVQIYDPASNHARAIRVDAGSLVGLAPAADGGLL
                     AASDLAAFHIAWPDPWSVIGTDAGLSGSVHGLAWWQGRWHALSGSGVYRDMAADPGEW
                     SFQRQPWTDHEAWALLALDEHSALLAESYTLQLIENGKARPLSSATLYPRMLYRSPFD
                     ADTVYIGTEMGVAVLRRERGSWHLVLEHSTPEQVLASSIVETAPRELWIGAERNGIQK
                     LRLSDDYRKVLQAESFGAAQGLSYGRLDNGGVYRLPEGALLAITASGMFRWDGQRFVA
                     DALGNLAQLREADESLALAHSPAGEVWAFSFKHAFRRLHDGQWRREEIANIRQGALES
                     LDFDPSGAVLLGDTNSILHFDARRLPASGHRPLVALRSVERLAPDGSLRPLTINAGGR
                     VTLKLGEAGLRFNYALPEFRGRAAPRYQVRLQGLDEKFSDWSDAKSISYLRIDPGEYS
                     FQVRARDAQGQISETMPLEFVVVPPWYERRWARALWWVLLPALAALLTAALNRARNAR
                     LKAEKARLEAMVAERTHDLEHANRRLDSLAHLDGLTEVPNRRRLDEYLLHVWGQCAEQ
                     QRWLSVLVIDVDRFKNYNDAHGHLAGDDVLKQIVRVLAHCLRRAEDLVARFGGDEFVV
                     VLPGAKRHAAAEIAESMRAHVEKSQAGATVSIGIASCEPARGGAVAELVHEADEALYQ
                     AKNTGRNRVAGGEPITSKATPV"
BASE COUNT          482 a         1046 c         1041 g          478 t
ORIGIN      
        1 tgcgcgtggt cgacaagttt ctcatgcagc tcgaggagca gctgggcgcc aagcacgtgc
       61 agctcgatgt ggacgaggaa gccaaggact ggatcgcgct gcgcggctac gacgtcaaga
      121 tgggcgcgcg gccgatggcg cgcgtgatcc aggaaaacct caagcgtccg ctggccgagg
      181 aactgctgtt cggcaagctg gccaacggcg gcaaggtcac cgtgacggtc aaggacgaca
      241 agctggaatt cgagatggaa acccgcgaca agccggtgac gctcgagccg gtgtaacctc
      301 accggggatg ctgcaaccag gcccgccgcg cgcgggcctt tttcatggtc agcgctacgg
      361 cgcgccgaat tttcgatcca cctcgagcag gcccttgccg ggcacgtggt tcatctccag
      421 gcggatgccg tcgggatctt cgaacagcac ggagtagtag cccggcgccc agcggtcttc
      481 ctgcgggcca tgcacgatgg tggcgccctg ctgctgcagc cacgcgtgca gcgcgtcgat
      541 gtcggcgcgc tcgcgtgcgc ggaagcacag gtgctgcagg cccacgcgcg cctgcacgaa
      601 gcgctggccg cggtactcgg ggtcggccac cttgatgccg agggcggtgc ggccgccgac
      661 gcagtagaaa tatcccggct ggtccagcac ctgcttcatg ccgagcagcg gcagcagctt
      721 gccgtagaac tcgcgcgccc gcgcgaagtc gccggtggtg aggatcacgt gcgcgatgcc
      781 attgatgtcc atgcgtatct ccgcgggccc gaaaacacct ccaccgcatg ccggataatg
      841 atgttataaa ttatttatca caatataaaa tatacaacaa aaaataacat gccaacagtg
      901 ggtgcggtgc cgtcaaaaag gcgatctgca atcacaccgg cgtggctttc gacgtgatcg
      961 gttcgccgcc ggccacgcgg ttgcggcccg tgttcttcgc ctggtacagc gcctcgtcgg
     1021 cctcgtgcac cagctcggcc acggcgccac cgcgggcggg ctcgcacgag gcgatgccga
     1081 tggagaccgt ggcgccggcc tggctcttct ccacgtgcgc gcgcatcgac tcggcgatct
     1141 ccgccgcggc gtggcgcttg gcgccgggca gcactacgac gaactcgtcg ccgccgaaac
     1201 gcgccaccag gtcttccgcg cggcgcaggc aatgcgccag cacgcgcacg atctgcttga
     1261 gcacgtcgtc gccggcgagg tggccgtgcg catcgttgta gttcttgaag cggtcgacgt
     1321 cgatcaccag caccgacagc cagcgctgct gttccgcgca ctggccccac acgtgcagca
     1381 ggtattcgtc gaggcggcgg cggttgggca cctcggtcag gccgtcgaga tgcgccaggc
     1441 tgtcgaggcg gcggttggcg tgttccaggt cgtgcgtgcg ctcggccacc atcgcttcga
     1501 gcctggcctt ctccgccttg agccgcgcgt tgcgcgcacg gttcagcgcg gcggttagca
     1561 gcgcagccag cgcgggcagg agcacccacc acagcgcacg ggcccagcgg cgttcgtacc
     1621 acggcggcac cacgacgaac tccagcggca tcgtctcgct gatctgcccc tgtgcgtcgc
     1681 gcgcgcgtac ctggaagctg tattcgcccg gatcgatgcg caggtaggag atcgacttgg
     1741 catcgctcca gtcggagaat ttttcgtcga gcccctgcag gcgcacctgg tagcgcggcg
     1801 cggcgcggcc gcggaactcc ggcagcgcgt agttgaagcg caggcccgct tcgcccagct
     1861 tgagcgtgac gcggccgccg gcgttgatgg tcagcggcct gagcgaacca tcgggcgcca
     1921 gccgctccac gctgcgcagc gccaccagcg gccggtgccc gctggccggc agccgccgcg
     1981 cgtcgaaatg caggatgctg ttggtgtcgc ccagcagcac cgcgcccgag ggatcgaagt
     2041 cgagagattc cagcgcgcct tggcggatgt tggcgatctc ctcgcgccgc cactgcccgt
     2101 catgcagccg gcggaacgca tgcttgaagc tgaaggccca cacctcgccg gccggcgagt
     2161 gcgccagtgc cagcgactcg tccgcctcgc gcagctgcgc caggttgccc agcgcatcgg
     2221 cgacgaagcg ctggccgtcc cagcggaaca tgccgctggc ggtgattgcc agcagcgcgc
     2281 cttccggcag ccggtagacg ccgccgttgt cgagccggcc gtagctcagg ccctgcgcgg
     2341 cgccgaagct ctcggcctgc agcaccttgc ggtagtcgtc ggacaggcgc agtttctgga
     2401 tgccgttgcg ctcggcgccg atccacaact cgcgcggcgc tgtttccacg atgctgctgg
     2461 ccagcacctg ctcgggggtg ctgtgctcca gcaccaggtg ccagctgccg cgctcgcggc
     2521 gcagcaccgc cacgcccatc tcggtgccaa tgtagaccgt gtcggcatcg aacggcgagc
     2581 ggtacagcat gcgcgggtag agtgtggcgg acgacagcgg ccgcgccttg ccgttttcga
     2641 tcagctgcaa ggtgtacgac tccgccagca gcgcgctgtg ctcgtccagc gccagtagcg
     2701 cccaggcttc gtggtcggtc catggctggc gctggaacga ccactcgccc ggatcggccg
     2761 ccatgtcgcg gtagactccg ctgcccgata gcgcatgcca gcgcccctgc caccaggcga
     2821 gcccatgcac cgaacccgac aggccggcgt cggtgccgat cacgctccac ggatcgggcc
     2881 aggcgatgtg gaacgcggcg aggtcgcttg ccgccagcag cccgccatcg gcggcgggcg
     2941 cgaggccgac cagcgaaccg gcgtcgacgc ggatggcgcg cgcatggttg ctggccggat
     3001 cgtagatctg cacgttgccg tcgtcgcccg ccagcgcgag ggtgccg
//
LOCUS       DASZZC010000794         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_888971,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000794 DASZZC010000000
VERSION     DASZZC010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888971"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..753)
                     /locus_tag="VHE37_17055"
     CDS             complement(22..753)
                     /locus_tag="VHE37_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37297.1"
                     /translation="MNPAAYQALRTMMAKGASKHQSQLSRAMENLIAAFDASPPPGWR
                     STVPYFDLREVRAVVRGQVLRDIGRAANAQLAVASIIGTSNPEFAIRRTPREWLEAAV
                     EQGELRQWVDLLVRVGPETVLAEHDRRMDALVLPDGNAIVHHGSMRWVLARLTAAEGT
                     FACVQCQCTADDTRASNALALYQQKHPQMKLTRTEAVNAPAQRFQFRGGEGELDVLSR
                     DELIARAGRLRSFFRVKSADDGKNA"
     gene            861..>1948
                     /locus_tag="VHE37_17060"
     CDS             861..>1948
                     /locus_tag="VHE37_17060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT37298.1"
                     /translation="MNRTHLSLAAALLLAACGGGKPAAAPAKGAGPSTPVVTAAVRQQ
                     AVPLTLESIGNVEAMASVAVKSLVDGQITEVKVKDGQDVTRGQPLFQIDPRPFQVQLA
                     TAQANLTRDQALLSTARAQEQRYRDLLAQGFVSKNDYATVQNNLDTAAASVTADQNAI
                     ETAKLQLDYADIRAPIGGRLGKIALQAGNLVKANDANPLVTLNQLDPIYVSFTVPETQ
                     FSALHAAMQGRQLAVAARDQQSSQTYTGQLTFIDNAVDTTTGTLRLRATFDNARGQLW
                     PGQFVDVTLTLSSDEVLTIAPEALQTGPKGQYVWVIDADHKAQLRDIVVARTTPDVAV
                     IARGLKAGEKVVIDGQSRLTPGALTAER"
BASE COUNT          329 a          690 c          656 g          273 t
ORIGIN      
        1 acacgcgaac cggccgtgcg gtcaggcgtt ttttccgtcg tccgccgact tcacgcggaa
       61 gaacgagcgc agccggcccg cgcgcgcgat cagttcgtcg cgcgacagca cgtccagttc
      121 gccttcgccg ccgcggaact ggaagcgctg cgccggcgca ttcaccgcct cggtgcgcgt
      181 gagcttcatc tgcggatgct tctgctggta cagcgccagc gcattgctgg cgcgcgtgtc
      241 gtcggccgtg cactggcact gcacgcaggc gaaggtgccc tccgcggcgg tgaggcgcgc
      301 cagcacccag cgcatgctgc cgtgatgcac gatggcattg ccgtcgggca gcaccaacgc
      361 gtccatgcgg cggtcgtgct cggccagcac cgtctccggt cccacgcgca ccagcaggtc
      421 gacccactgg cgcagctcgc cctgttcgac ggccgcttcc agccattcgc gcggcgtgcg
      481 gcggatggcg aactccgggt tggaagtgcc gatgatgctg gccaccgcca gctgcgcgtt
      541 ggcggcgcgg ccgatgtcgc gcagcacctg tccgcgcacc acggcacgca cttcacgcag
      601 gtcgaaatac ggcaccgtgc tgcgccagcc cggcggcggg ctggcatcga acgcggcgat
      661 caggttttcc atggcgcgcg acagctgcga ctggtgcttg ctggcaccct tggccatcat
      721 cgtgcgcagc gcctggtagg cggccggatt catgcggcca ttgtcgccaa acgcgcaaac
      781 tattgttaag cggtcttttc ataatcgcgc gcaaccggca cagtgccgcc gcggtcatac
      841 ccccgaaccc gccgagaaca atgaacagaa cccatctcag cctggccgca gccctgctgt
      901 tggctgcctg cggcggcggc aagcccgcgg ccgcaccggc caagggcgcc ggcccgtcca
      961 cgccggtggt cacggcagcg gtgcgccagc aggcggtgcc gctcacgctg gagagcatcg
     1021 gcaacgtcga ggccatggcc agcgtggcgg tgaagtcgct ggtcgacggc cagatcaccg
     1081 aagtgaaggt gaaggacggc caggacgtga cgcgcggcca gccgctgttc cagatcgacc
     1141 cgcggccgtt ccaggtgcag ctcgccaccg cgcaggccaa cctgacgcgc gaccaggcac
     1201 tgttgtccac cgcgagggcg caggagcaac gctaccggga cctgctggcg cagggcttcg
     1261 tgtcgaagaa cgactacgcc acggtgcaga acaatctcga caccgccgcg gcctcggtca
     1321 ccgccgacca gaacgcgatc gagacggcga agctgcagct ggactacgcc gacatccgtg
     1381 cgccgatcgg cgggcgcctg ggcaagatcg cgctgcaggc cggcaacctg gtcaaggcca
     1441 acgacgccaa cccgctggtc acgctcaacc agctcgatcc gatctacgtc agcttcacgg
     1501 tgccggaaac gcagttctcc gcgctgcatg cagcgatgca gggcaggcaa ctggcggtgg
     1561 ccgcgcgcga ccagcagtcg agccagacct acaccgggca gctgaccttc atcgacaacg
     1621 cggtcgacac caccaccggc acgctgcgcc tgcgcgccac cttcgacaac gcgcgcggcc
     1681 agctgtggcc cggacagttc gtcgacgtga cgctgacgct gtcgtccgac gaagtgctga
     1741 ccattgcgcc ggaagcgctg cagaccggac ccaagggcca gtacgtgtgg gtgatcgacg
     1801 ccgaccacaa ggcgcagttg cgcgacatcg tcgtggcgcg caccacgccg gacgtggcgg
     1861 tgatcgcgcg cgggctcaag gcgggcgaga aagtggtgat cgatggccaa tcgcggctga
     1921 ccccgggcgc gctgaccgcc gagcgcag
//
LOCUS       DASZZC010000795         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_445098,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000795 DASZZC010000000
VERSION     DASZZC010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445098"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VHE37_17065"
     CDS             <1..180
                     /locus_tag="VHE37_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37299.1"
                     /translation="HALDQVARVLVKHAEVGICGGGELDHGGNERALMIIPTLARCRR
                     FADNKRTTLPEMHRA"
     gene            177..1211
                     /locus_tag="VHE37_17070"
     CDS             177..1211
                     /locus_tag="VHE37_17070"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HVT37300.1"
                     /translation="MKLITALLAVLLAGPAVAAQQQTMWVCYDDHGRRSVQDHPCDSA
                     QEQPPASGAPLGTALPQSGGSGQPAPTPIEPLPQHQPRAADASGAVPAAMAPGDFDDF
                     FASHRNELIALAVLTGFVMLLLAGGSLLLRRMAARLRAHADERRVAPARIEASPELLR
                     AWKLAPAREEPLLPATPSAGSAARMPPIPMVPMPPPAAPRPAAEVPRRVAATALTLER
                     LRLLDRPQLEALCVGYWKIKGYAAAQVNNHIHLFQPAEPSKIFALVKCRAGTEPVSAA
                     QVRELWAAAHHLGVRLVIVYSLAGFSDDAQAYAQGKHLKLFSGADLVNQIRALTDDQR
                     RELPDPMAPA"
     gene            1208..1873
                     /locus_tag="VHE37_17075"
     CDS             1208..1873
                     /locus_tag="VHE37_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37301.1"
                     /translation="MKVSYKFVFGDGSEHRIEVDLERAPPSGVAQPQWTQLAFRQCPN
                     CPLKPEQQAHCPAAVDLAPTVQAFAHIISHHEAQVVVETPERVVGKACQVQTALQSVA
                     ALIMATSACPILGRMRGLAYTHLPFATLEESMFRVLGSYLLRQLLIHRNGGEPDWELE
                     GLKAHYADLEILNRAFKARIDAAAEQDAAINAVSALGVLSMGIGFSIDDQLPELARLA
                     VTA"
     gene            1870..3060
                     /locus_tag="VHE37_17080"
     CDS             1870..3060
                     /locus_tag="VHE37_17080"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVT37302.1"
                     /translation="MISRLATIFVALGSCVSCGAASAPGSDGLPAPDLSYRGVRTSKV
                     LGWPAGRTPAVPPGFAITAWADHIPDPRKVYFAPNGDAFIADSRSGRVLLLRDRDGNH
                     YPDHGEVYAEGLNYPYGMLVLGDSFYIGAADAVWKFPYKAGDLHAAAGRKILTLPDHG
                     HVTRNLCASRNGQKLYVAVGSLSNVADAGMDKEFHRANILEINPDGSGLRVYAAGLRN
                     PVGLALRGDTLWTTVNERDMLGADLPPDYLTHLVDGSFYGWPYAYWGAHEDPRRAGER
                     TDMVRRTTVPDAALGAHVAPLGFAFYDAALLPAQYRGGVFVAEHGSWNRPQFAGYKVV
                     YLPFAHGAPGGPPQDFATGFIADGDKSEVYGRPADVAIAPDGALLIADDAGQTIWRVV
                     PRAP"
     gene            complement(3063..4271)
                     /locus_tag="VHE37_17085"
     CDS             complement(3063..4271)
                     /locus_tag="VHE37_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT37303.1"
                     /translation="MSPASADHRRLAVVLAGAAAFVNLYAPQSLLPVLQKWIGNADAA
                     LAGLIVSAGTLGVALAAPFAGLLADRLGKRRVIVLAALLVVIPGAAIPLAQTPQQLIA
                     ARFIEGLFLPGIFAVTVAYIGSEFPVAQARAVTSLYVAGTIGGGFCGRLLSGLVTEFA
                     GWRWGFAALALMQLLLALAIRAWLPAERPAAHPHVSSLATLPALLRRPELRGVFVVGF
                     MILFALVGGFTYVSLRLAQPPFSLGPAALSAIFVVYLVSMTITALSGVLLNRYGHARV
                     LALAWGITVLGLLLSLVPVLWVVCLGLMLFSAGLFIAQTTSTSFVGEAAGAARAPAVG
                     LYVTCYYAGGSVGGVLPAPLWNHYGWSGVVALLCIAGVLSVSIGWRSFQHAARPAPSD
                     PAFEAGGEAP"
     gene            4310..>4712
                     /locus_tag="VHE37_17090"
     CDS             4310..>4712
                     /locus_tag="VHE37_17090"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HVT37304.1"
                     /translation="MSEPKPLTYMSYLKLDELLSLQQPLSTGPEHDETLFIVIHQVYE
                     LWFKQMLHELRHLRSCLQQRDTPRALATFKRVLTILKTLVAQVDVIETITPLSFNAFR
                     ARLEASSGFQSWQFRALEFILGHKRAGLLAQY"
BASE COUNT          768 a         1639 c         1631 g          674 t
ORIGIN      
        1 catgcgctcg atcaggtcgc gcgcgtgctc gtcaagcatg ctgaagtcgg catctgcggg
       61 ggaggagaac ttgatcatgg cgggaatgaa cgtgcgctga tgatcattcc gaccttggcg
      121 cggtgtcggc ggttcgccga caataaacgc acaaccttgc cggagatgca ccgggcatga
      181 agctcatcac cgcgctgctg gccgtgctgc tcgccgggcc ggccgtcgcc gcgcagcagc
      241 agaccatgtg ggtgtgctac gacgatcacg gccgccgctc ggtgcaggat catccgtgcg
      301 acagcgcgca ggaacagccg ccggccagcg gcgcgcccct gggcacggcg ctgccgcaga
      361 gcggcggcag cggccagccg gcgcccacac cgatcgaacc gttgccgcaa caccagccgc
      421 gcgccgccga tgcgtcgggc gcagttcccg cggcgatggc gcccggagat ttcgacgatt
      481 tctttgccag ccaccgcaac gagctgatcg cgctggccgt gctcaccggc ttcgtcatgc
      541 tgctgctcgc gggcggcagc ctgctgctgc gccgcatggc cgcgcgcctg cgcgcgcacg
      601 ccgacgagcg ccgcgtggcg ccggcgcgca tcgaggcttc gcctgaactg ttgcgcgcct
      661 ggaagctggc gcctgcgcgc gaggagccgc tgctgccggc aacgcccagc gccggcagtg
      721 ctgcgcgcat gccgccgatt cccatggtgc cgatgccgcc gccggctgcg ccgcggcccg
      781 ccgccgaagt gccgcggcgc gtggccgcga cggcgctgac gctggaacgc ctgcgcctgc
      841 tcgaccgtcc gcagctcgag gcgctgtgcg tcggttactg gaagatcaag ggctacgccg
      901 cggcgcaggt gaacaaccac atccacctgt tccagccggc ggagccgtcg aagatcttcg
      961 cgctggtcaa atgccgcgcc ggcaccgagc cggtgagcgc ggcgcaggtg cgcgagctgt
     1021 gggcggcggc gcaccacctg ggcgtgcgcc tggtgatcgt ctacagcctg gccggtttca
     1081 gcgacgatgc gcaggcctat gcgcagggca agcacctcaa gctgttcagc ggtgccgacc
     1141 tggtcaacca gatccgcgca ctcaccgacg accagcggcg cgagctgccg gacccgatgg
     1201 cgccggcatg aaggtcagtt acaagttcgt gttcggcgac ggcagcgagc atcgcatcga
     1261 ggtcgatctc gagcgtgcgc cgccatcggg tgttgcgcag ccgcaatgga cccagctggc
     1321 gttccgccag tgccccaact gtccgctcaa gcccgaacaa caggctcact gtccggcggc
     1381 cgtcgacctg gcgccgacgg tgcaggcgtt cgcgcacatc atttcgcacc acgaggccca
     1441 ggtggtagtg gagacgccgg agcgggtggt cggcaaggcc tgccaggtgc agaccgcact
     1501 gcaatcggtg gcggcgctga tcatggccac cagcgcctgc ccgatcctcg gccgcatgcg
     1561 cgggctggcc tacacccacc tgccgttcgc cacgctggag gagagcatgt tccgcgtgct
     1621 cggcagctac ctgctgcgcc agctgctgat ccaccgcaac ggcggcgagc cggactggga
     1681 gctggaaggg ctgaaggcgc actacgccga cctggaaatc ctcaaccgcg ccttcaaggc
     1741 gcggatcgac gccgccgcgg agcaggatgc ggcaatcaac gcggtatcgg cgctgggcgt
     1801 gctgtcgatg ggcatcggct tttccatcga tgaccagctg ccggagctgg cgcggctggc
     1861 cgtgaccgcg tgatcagccg gcttgctacg attttcgtag cactgggaag ctgcgtatcc
     1921 tgcggcgcgg cgtccgcgcc gggcagcgac ggcctgccgg cgccggatct gtcctatcgc
     1981 ggcgtgcgta ccagcaaggt gctcggctgg ccggccgggc gcacgccggc ggtgcctccc
     2041 ggtttcgcca tcacggcctg ggccgaccat attcccgatc cgcgcaaggt ctacttcgcg
     2101 cccaacggcg atgccttcat cgccgacagc cgcagcgggc gcgtgctgct gctgcgcgac
     2161 cgcgacggca accattatcc cgaccatggc gaggtgtatg ccgagggtct caactatccg
     2221 tacggcatgc tggtgctggg cgactcgttc tacatcgggg cagcggacgc ggtgtggaag
     2281 ttcccgtaca aggccggtga cctgcatgca gcggccggcc gcaagatttt gaccttgccc
     2341 gaccacggtc acgtcacgcg caacctctgc gcgtcgcgca acggccagaa gctgtacgtg
     2401 gcggtcggct cgttgagcaa cgtggccgat gccgggatgg acaaggaatt ccatcgcgcc
     2461 aacatcctcg agatcaatcc tgacggcagc ggcctgcgcg tgtacgcggc cgggttgcgc
     2521 aatcccgtcg ggctggcctt gcgcggcgac acgctgtgga ccacggtgaa cgagcgcgac
     2581 atgctcggcg ccgacctgcc gccggactat ctcacgcacc tggtcgacgg ctcgttctac
     2641 ggctggccct acgcctactg gggtgcgcat gaagatccgc gccgtgccgg cgagcggacc
     2701 gacatggtcc gccgcaccac ggtgcccgat gccgcgctgg gcgcccacgt ggcgccgctg
     2761 ggcttcgcct tctacgacgc cgcgctgctg ccggcgcaat accgcggcgg cgtgttcgtc
     2821 gccgagcatg gctcgtggaa ccgtccgcag ttcgccggat acaaggtggt gtacctgccg
     2881 ttcgcgcatg gcgcgcccgg cgggccgccg caggatttcg ccaccggctt cattgccgac
     2941 ggcgacaaga gcgaggtcta tggccgcccg gcggatgttg cgattgcgcc ggacggcgcg
     3001 ctgctgatcg ccgacgatgc cggccagacc atctggcgcg tggtgccgcg cgcgccctaa
     3061 aatcagggcg cttcgccacc cgcctcgaat gcgggatcgc tcggcgccgg ccgcgccgca
     3121 tgctggaacg aacgccagcc gatgctcacc gagagaaccc cggcgatgca cagcagcgcg
     3181 accaccccgc tccagccgta gtggttccac agcggcgccg gcagcacgcc gccgacgctg
     3241 ccgccggcgt agtagcaggt cacgtacaag ccgaccgcgg gcgcgcgcgc ggcgccggcc
     3301 gcttcgccga cgaaactggt ggacgtggtc tgcgcgatga acagtcctgc cgagaacagc
     3361 atcagcccga gacagaccac ccacagcacc ggcaccaggc tgagcaggag tccgagcacg
     3421 gtgatgcccc aggccagcgc cagcacgcgc gcgtggccgt agcggttgag cagcacgccg
     3481 gacagcgccg tgatggtcat gctgaccagg tagacgacga agattgccga tagcgctgcc
     3541 gggccgagcg agaacggcgg ctgcgccagg cgcagcgaca cgtaggtgaa gccgccgacc
     3601 agtgcgaaca ggatcatgaa gcccaccacg aacacgccgc gcagttccgg ccggcgcagc
     3661 agcgcgggca gcgtggccag cgatgagaca tgcggatgcg cggccgggcg ttccgccggc
     3721 agccatgcgc ggatggccag cgccagcagc agctgcatca gcgccagcgc ggcaaaaccc
     3781 cagcgccagc cggcaaactc ggtgaccagg cccgagagca ggcgcccgca gaagccaccg
     3841 cctatcgtgc cggcgacgta cagcgaggtg accgcgcgcg cctgcgccac cgggaattcg
     3901 ctgccgatgt aggcgacggt gacggcaaag attcccggca ggaacagtcc ctcgatgaag
     3961 cgcgccgcga tcagctgctg cggcgtctgc gccagcggga tggccgcgcc gggtatcacc
     4021 accagcagcg cggcgagcac gatcacgcgg cgcttgccca gccggtcggc cagcaggccg
     4081 gcgaacggtg cggccagcgc caccccgagc gtgccggccg atacgatcag cccggccagc
     4141 gcggcgtcgg cgttaccgat ccatttctgc agcaccggca gcagggactg cggtgcgtag
     4201 aggttgacga aggccgcagc gccggcgagg acgacggcga gtcggcggtg atcggcggag
     4261 gcggggctca tcgggcgcgc actctagctg atggtgagag aatcgcggca tgagcgaacc
     4321 caagccgctg acctacatga gctacctgaa gctcgatgag ctgctgtcgc tgcagcagcc
     4381 gttgagcacg ggcccggaac acgacgagac cctgttcatc gtcatccacc aagtgtacga
     4441 gctgtggttc aagcagatgc tgcacgagtt gcgtcacctg cgttcctgcc tgcagcagcg
     4501 cgacacgccg cgcgcgttgg cgacattcaa gcgcgtactg accattttga agacgctggt
     4561 ggcgcaggtc gacgtgatcg agaccatcac gccgctgtcg ttcaacgcct tccgcgcgcg
     4621 gctggaagcg tccagcgggt tccagtcctg gcagttccgc gcgctggaat tcatccttgg
     4681 acacaagcgc gcaggcctgc tcgcgcaata tc
//
LOCUS       DASZZC010000796         6416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_889377,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000796 DASZZC010000000
VERSION     DASZZC010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889377"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="ruvB"
                     /locus_tag="VHE37_17095"
     CDS             complement(<1..755)
                     /gene="ruvB"
                     /locus_tag="VHE37_17095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HVT37305.1"
                     /translation="MSEERVISAKAAGDEERIERAIRPAKLADYVGQPAVREQLSIAI
                     EAARGRGEALDHVLIFGPPGLGKTTLAHILAAEMGVNLRQTSGPVLERAGDLAALLTN
                     LEPRDILFVDEIHRLSPVVEEVLYPALEDFQLDIMIGEGPAARSIRLDLPPFTLVGAT
                     TRAGALTSPLRDRFGIVQRLEFYSVPDLTHIVTRSAGILKAQIDDGGAREIAQRSRGT
                     PRIATRQLRRVREYAQGRGQGIVTQAMAQEAMKL"
     gene            complement(752..1339)
                     /gene="ruvA"
                     /locus_tag="VHE37_17100"
     CDS             complement(752..1339)
                     /gene="ruvA"
                     /locus_tag="VHE37_17100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HVT37306.1"
                     /translation="MIGRLTGKLVQKQPPLLLLDVNGVGYEIEAPMSTFYKLPATGET
                     VTLHTHLTVREDAHLLFGFATHAEKDLFRALIKLNGVGPKLALSVLSGMSVEEFWITV
                     RAQDAVRLTKLPGVGKKTAERLVLEMRDKAGAPESAQLPGAPAAQASPLQEARAALAA
                     LGYKPAEIERYTQAVYKDGMTVEQIIQEALKRAVR"
     gene            complement(1344..1856)
                     /gene="ruvC"
                     /locus_tag="VHE37_17105"
     CDS             complement(1344..1856)
                     /gene="ruvC"
                     /locus_tag="VHE37_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVT37307.1"
                     /translation="MTRILGIDPGSRHTGYGLIECASNRNRHLAHGRIDTLEGEMPQR
                     LLAILNGLTDLIREYQPDEIAIEETFVNRANPASALVLGQARGAAICACAQAGLPLAE
                     YAAAQVKVAIAGNGRAEKQQVQHMVKVLLNLRAALPLDASDALAVALTHAHVRGTRLK
                     TGVTLRAAWG"
     gene            complement(1853..2575)
                     /locus_tag="VHE37_17110"
     CDS             complement(1853..2575)
                     /locus_tag="VHE37_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVT37308.1"
                     /translation="MGRGPSIEGRKNAEDAKRAKVFTKLIREITVAARSGGGDPAGNP
                     RLRLCMDKALAANMPKDTLNRAIKRGTGEGSTEAIEEIRYEGYAPGGVALIVDCMTDN
                     PTRTVADVRHAFSKHGGHLGTAGSVAYLFSRRGDIAIDTRGDAGLPEKIMEVAIEAGA
                     DDVIDEAGYTEVLTAPENFEAVKQALEKAGLKPYRADIALRPSTRVAVGGETAQAVGK
                     LIEVLEDLDDVQNVYTNADLPE"
     gene            complement(2604..3182)
                     /locus_tag="VHE37_17115"
     CDS             complement(2604..3182)
                     /locus_tag="VHE37_17115"
                     /inference="COORDINATES: protein motif:HMM:NF019081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HVT37309.1"
                     /translation="MKRVLAILGIAIGAVALPAQAEIVDFNIGDNAFRLDLAGPLSRL
                     FSGVSGQYDIGALLKPRTSDNLIQVHTGVLLTGDVGAKGIDLAAGLGGRIVYTGRNHD
                     SGGAFAPGVQLEARLPEFNRFGTTFYGYFGPSVTSFGEVRKYQEYGLDLDYAVIKGGS
                     VYVGWRSIKEDFEDQNSYTVDRGIHGGFRLKF"
     gene            complement(3248..4687)
                     /gene="lpdA"
                     /locus_tag="VHE37_17120"
     CDS             complement(3248..4687)
                     /gene="lpdA"
                     /locus_tag="VHE37_17120"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HVT37310.1"
                     /translation="MSDYDVIVVGGGPGGYPCAIRAGQLGFKVACIDAWINKDNTPAF
                     GGTCLNAGCIPSKALLESSENYHRAQHEFAAHGINVGSISVDIAKMQARKQAVSKSLT
                     SGIKTLFTANKVTGLHGTGKLLGNGQVEYTAADGKKQTLTAKHIVIATGSEPVNLSKV
                     APFDGENIVDSWGALDFTAVPKTLGIIGAGVIGVELGSVWQRLGAKVTILEALPNFLP
                     MVDAAVSKEALRQLAKQGLDIRLSAKVLSAKAGKSGVAVEYEMGGEKKTETFEKLIVA
                     VGRRPYTKGLNAEGVGVALDERGFVKVDTHTYKTNVPGIYAIGDVIGGAMLAHKGIEE
                     GVALAEQLAGHHTQVNYAAIPSVIYTAPEIAWVGLTEEQAKAAGHEVKVGSGPFAASG
                     RAKAMEQPVGTIKVIADAKNDRILGVHMCGPYVSELLAEAVLGMEFAATCEDIALTMH
                     AHPSLSETFHEAVLSVDGRAIHAVNKVKK"
     gene            complement(4789..5667)
                     /locus_tag="VHE37_17125"
     CDS             complement(4789..5667)
                     /locus_tag="VHE37_17125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HVT37311.1"
                     /translation="MDGTAPDSRAIVPRKGPDFGFDGDVPRHWLDNDPFKTRFFDAMS
                     CLFPEGEKFFISCVRDYRDQITDPKLAAEVKDFIYQEGQHGMVHTQFNNRLRAQGVEV
                     DRILKKQNDIMYGFFRKRFPRSFTLGQTAAAEHMTALMAHGFFSHDFMKDADPRVRAM
                     YAWHAVEEIEHKAVAYDVLKTVAKAGYFTRIASFMLTTFTFPFHVFMIMQHMFKVDGV
                     KNKPWVWLKGLWWLYGWNGLYPRLMPHYLLYFLPGFHPWKFGDMRIYETWTRAYAKDG
                     AIAAGDAVALAGGSAA"
     gene            complement(5828..>6416)
                     /locus_tag="VHE37_17130"
     CDS             complement(5828..>6416)
                     /locus_tag="VHE37_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT37312.1"
                     /translation="TRDQFRVDKLTFRYRAPKYAAEYKKFFGLPIEFGRRENAAVFGR
                     ELLDAKLGGSFPTLHRQAEFLVEQRIRRLPKKLGLVAAIEGAFADNPALLGQSVDALA
                     RTLGLHTRALQRKLREEKQSYGELLAHARHRLAMQLLETGGESIDRIGEQLGFSDRRS
                     FTRAFTRWNGSTPSAFRQRGRKKSPDRTASKHVIK"
BASE COUNT         1012 a         2139 c         2073 g         1192 t
ORIGIN      
        1 agcttcatcg cctcctgcgc catggcctgg gtgacgatgc cctggccgcg cccctgcgcg
       61 tactcgcgca cgcgccggag ctggcgggtg gcgatgcgcg gcgtgccgcg cgagcgctgc
      121 gcgatctcgc gcgcgccgcc gtcgtcgatc tgcgccttca ggattcccgc gctgcgcgtg
      181 acgatgtgcg tgagatcggg caccgagtag aactccagcc gctgcacgat gccgaagcgg
      241 tcacgcagcg ggctggtcag cgcgccggcg cgcgtggtgg cgcccaccag cgtgaacggc
      301 ggcaggtcga ggcggatcga gcgcgcggcc gggccttcac cgatcatgat gtcgagctgg
      361 aagtcctcca gcgccgggta cagcacttcc tcgaccaccg gcgacaggcg atggatctcg
      421 tcgacgaaca ggatgtcgcg cggctcgagg ttggtcagca gcgcagccag atcgcccgca
      481 cgctccagca ccgggccgga ggtctggcgc aggttcacgc ccatctccgc cgcgagaata
      541 tgcgcgagcg tggtcttgcc caggcccggc ggaccgaaga tcagcacgtg gtccagcgcc
      601 tcgccgcggc cgcgcgccgc ctcgatggcg atggacagct gctcacgcac cgccggctgg
      661 cccacgtagt cagccagctt ggccggacgg atggcgcgct cgatgcgctc ctcgtcgccg
      721 gccgccttgg cggagatgac gcgctcttcg ctcatcgcac cgcgcgcttg agcgcttcct
      781 ggatgatctg ctcgacggtc atgccgtctt tatacacggc ctgcgtgtag cgctcgatct
      841 ccgccggctt gtagccgagc gcggccagcg cggcgcgcgc ttcctgcaac ggcgaggcct
      901 gcgccgccgg cgcaccgggc agctgtgccg actccggcgc gcccgccttg tcgcgcattt
      961 ccagcaccag gcgctcggca gtcttcttgc ccacgcccgg cagcttggtc aggcgcacag
     1021 cgtcctgcgc gcgcacggtg atccagaatt cctccaccga catgcccgac agcaccgaca
     1081 acgcgagctt ggggccgacg ccgttgagct tgatcagcgc acggaacagg tccttctcgg
     1141 cgtgtgtggc aaagccgaac agcaggtgcg cgtcctcgcg cacagtgaga tgcgtgtgca
     1201 gggtcactgt ctcgccggtg gcgggcagtt tgtagaaggt cgacatcggc gcttcgatct
     1261 catagccgac gccgttcacg tcgagcagca gcagcggcgg ctgcttctgc accagcttgc
     1321 cggtgagcct gccgatcacg gttctaaccc caggccgcgc gcagcgtcac gccggtcttc
     1381 aggcgcgtgc cgcgcacatg cgcatgcgtg agcgcaaccg ccagtgcatc ggatgcgtcc
     1441 agcggcagtg ccgcgcgcag gttgagcagc accttgacca tgtgctgcac ctgctgcttc
     1501 tccgcgcggc cattaccggc aatggccacc ttgacctgcg ctgcggcgta ctcggccagc
     1561 ggcaagcccg cctgagcaca ggcgcagatg gccgcaccgc gcgcctgccc gagcaccagc
     1621 gcgctggccg ggttggcacg gttgacgaag gtttcctcga tggcgatctc gtccggctgg
     1681 tattcgcgga tcaggtcggt gaggccgttg aggatggcca gcaggcgctg cggcatctca
     1741 ccttcgagcg tatcgatgcg cccgtgtgcg agatggcggt tgcggttgga tgcgcactcg
     1801 atcagcccgt agccggtgtg gcgcgagccg ggatcgatgc cgaggatgcg cgtcactcgg
     1861 ggagatcagc gttggtgtag acgttttgca cgtcgtcgag gtcctcgaga acctcgatca
     1921 gcttgcccac ggcctgcgcc gtttccccgc ccaccgcgac ccgcgtcgac ggccgcaggg
     1981 cgatgtcggc gcggtacggc ttgagcccgg ccttttccag ggcctgcttc accgcctcga
     2041 aattttccgg cgcggtgagc acctcggtgt agccggcctc gtcgatcacg tcatccgcgc
     2101 cggcctcgat cgctacttcc atgatttttt ccggcaaccc agcatcgccg cgcgtgtcga
     2161 tggcaatgtc gccgcggcgc gagaacaggt aggccaccga accggcggtg cccaggtgcc
     2221 cgccatgctt ggagaaggca tggcgtacat cggccacggt gcgcgtcggg ttgtcagtca
     2281 tgcagtcgac gatcagcgcc acgccgccgg gcgcgtagcc ctcgtagcgg atttcctcga
     2341 tcgcctcggt gctgccctcg ccggtgccgc gcttgatggc ccggttgagc gtgtccttgg
     2401 gcatgttcgc ggccagcgcc ttgtccatgc acaggcgcaa gcgcggatta ccggcaggat
     2461 caccgccgcc gctgcgcgcg gcgacggtga tctcgcggat cagcttggtg aagaccttgg
     2521 cgcgcttggc atcttccgcg ttcttgcggc cttcgatcga cgggccacgg cccatgacat
     2581 gctcctgcgc gccggtgctg cgatcagaac ttcaggcgga aaccgccgtg gatgccgcgg
     2641 tcgaccgtgt agctgttctg gtcttcgaag tcttccttga tgctgcgcca gcccacgtag
     2701 accgaaccgc ccttgatcac ggcgtagtcg agatcaaggc cgtactcctg gtacttgcgc
     2761 acctcgccga acgaagtgac cgaagggccg aagtagccat agaacgtggt gccaaagcgg
     2821 ttgaactcgg gcaggcgcgc ttccagctgc acgcccgggg cgaaggcgcc gccgctgtcg
     2881 tgattgcggc cggtgtagac gatgcggccg cccaggcccg ccgcgagatc gatgcccttg
     2941 gcgccgacat caccggtgag cagcacgccg gtgtgcacct ggatcaggtt gtcgctggtg
     3001 cgcggcttga gcaatgcgcc gatgtcgtac tggcccgaga cgcccgagaa caggcgcgac
     3061 agcgggccgg ccaggtcgag gcggaaggcg ttgtcgccga tgttgaagtc cacgatctcc
     3121 gcctgcgccg gcagcgcgac ggctccaatt gcaatcccca gaattgccaa aacgcgtttc
     3181 atcgaattct ccatgtggat cgattttgca gcgttgataa tttgtagcct attacaacga
     3241 ttgtagatta tttcttgacc ttgttcacgg catgtatcgc ccgcccatcc acggacagca
     3301 cggcctcgtg gaaagtctcc gacagcgacg gatgcgcgtg catggtcagc gcgatgtctt
     3361 cgcaggtggc cgcgaattcc atgcccagca ccgcttcggc cagcagttcc gaaacgtacg
     3421 gcccgcacat gtggacgccc aggatgcggt cgttcttggc atcggcgatg accttgatgg
     3481 tgcccaccgg ctgctccatc gccttggcgc ggcccgacgc ggcgaatggg ccggaaccga
     3541 ctttcacctc gtgcccggcc gccttggcct gttcctcggt gagaccgacc caggcgattt
     3601 ccggcgcggt gtagatcacc gacgggatcg ccgcgtagtt cacctgcgtg tgatggccgg
     3661 ccagctgctc ggccagcgcc acgccttcct cgataccctt gtgcgccagc atggcgccgc
     3721 cgatgacatc accgatggcg tagatgcccg gcacgttggt cttgtaggtg tgagtatcga
     3781 ccttgacgaa accgcgctcg tccagcgcca cgcccacgcc ttccgcgttg aggcccttgg
     3841 tgtacgggcg gcggccgacg gcgacgatca gtttctcgaa tgtctcggtc ttcttctccc
     3901 cgcccatctc gtattcgacg gcgacgccgc tcttgccagc cttggccgac agcaccttgg
     3961 ccgacaggcg gatgtcgaga ccctgcttgg ccagctggcg cagcgcttcc ttgctgacgg
     4021 cggcatcgac catcggcagg aaattcggca gcgcctcgag aatggtgacc ttggcgccca
     4081 ggcgctgcca cacgctgccc agttccacgc cgatgacgcc ggcgccgatg atgcccagcg
     4141 tcttgggcac ggcggtgaaa tccagcgcgc cccaggaatc gacaatgttc tcgccgtcga
     4201 acggcgccac cttgctcagg ttcaccggct cgctgccggt ggcgatgacg atgtgcttgg
     4261 ccgtcagcgt ctgcttcttg ccgtcggccg cggtgtattc cacctggccg ttgcccagca
     4321 gcttgccggt gccgtgcagt ccggtgacct tgttggcggt gaacagcgtc ttgatgccgg
     4381 aagtcaggct cttgctcacc gcctgcttgc gcgcctgcat cttggcgatg tcgacgctga
     4441 tgctgcccac gttgatgccg tgcgcggcga actcgtgctg cgcgcggtga tagttctcgc
     4501 tcgattcgag caacgccttg gacgggatgc agcccgcgtt caggcaggtg ccgccgaacg
     4561 ccggcgtgtt gtccttgttg atccaggcgt cgatgcacgc caccttgaag ccaagctgcc
     4621 ccgcgcggat tgcgcaggga taaccgcccg gaccgccgcc gacgacgatg acgtcgtaat
     4681 cgctcattac gctccccggt ttttgaggcg gcctattcta atggaacgtt gcgcgctcca
     4741 taggggagaa acccatcttg ccccgcgctg cgggacgtgc cgtggcgttt acgccgcgga
     4801 accgccggcc agcgccaccg catcgcccgc cgcgatcgca ccatccttcg cgtacgcgcg
     4861 cgtccaggtt tcgtagatgc gcatgtcgcc gaatttccac gggtgaaagc cgggcaaaaa
     4921 gtacagcagg taatgcggca tcaggcgcgg atacaagccg ttccagccgt acagccacca
     4981 caggcccttg agccagaccc agggtttgtt cttcacgccg tccaccttga acatgtgctg
     5041 catgatcatg aacacgtgga acgggaaggt gaacgtggtc agcatgaatg aggcgatgcg
     5101 cgtgaagtag cccgccttgg ccaccgtctt gagcacgtcg taggccactg ccttgtgctc
     5161 gatctcctcc accgcgtgcc acgcgtacat ggcgcgcacg cgcggatcgg cgtccttcat
     5221 gaagtcatgc gagaaaaagc catgcgccat cagcgcggtc atgtgctcgg cggcggcggt
     5281 ctggcccagc gtgaagctgc gcggaaagcg cttgcggaag aagccataca tgatgtcgtt
     5341 ctgcttcttc aggatgcggt caacttccac accctgtgcc cgcagccggt tgttgaactg
     5401 cgtatgcacc atgccgtgct ggccttcctg gtagatgaag tccttcacct cggcggccag
     5461 cttgggatcg gtgatctggt cgcggtagtc gcgcacgcag gagatgaaga acttctcgcc
     5521 ttccgggaac aggcaactca tggcgtcgaa gaaacgcgtc ttgaacggat cgttgtccag
     5581 ccagtggcgc ggcacgtcgc cgtcgaagcc gaagtccggg cccttgcgcg gaacgatcgc
     5641 gcggctgtcg ggtgctgttc catccatgac cgctctccaa aaatttctct gagcgtgaat
     5701 ctaggaccgc cgcgcggcga atgctatgac cgctggcgac gttagggaac attttgcgac
     5761 atgaaaaccg ccgaccgcac cccgctccag cgtgttatta aatattaatc aatttaaaat
     5821 taatacatca tttaataaca tgcttgctag cggtgcggtc gggtgatttt ttccgaccgc
     5881 gctggcggaa cgcgctcggc gtgctgccgt tccagcgcgt gaaggcgcgg gtgaagctgc
     5941 gccggtcgga aaaacccagc tgctccccga tgcggtcgat gctttcgccg ccggtctcca
     6001 gcagttgcat ggccagccgg tgccgcgcgt gcgccagcaa ctcaccgtag ctctgctttt
     6061 cctcgcgcag cttgcgctgc agcgcacgcg tgtgcaggcc cagggtgcgc gccagcgcat
     6121 cgacgctctg cccgagcagc gccgggttgt ccgcgaaggc tccctcgatg gcggcgacca
     6181 ggcccagctt cttcggcagc ctgcggatgc gctgctcgac caggaactcc gcctgccggt
     6241 gcagcgtggg gaacgagccg ccgagcttcg catcgagcag ttcgcgccca aacaccgccg
     6301 cgttctcgcg ccggccgaac tcgatcggca ggccgaaaaa ttttttgtac tcggccgcgt
     6361 acttcggcgc gcggtagcgg aaggtgagct tgtccacgcg gaactggtcg cgggtc
//
LOCUS       DASZZC010000797         5323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_705253,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000797 DASZZC010000000
VERSION     DASZZC010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705253"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VHE37_17135"
     CDS             <1..233
                     /locus_tag="VHE37_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HVT37313.1"
                     /translation="KLLLDPPRSGAAAVLDYLPHKATRRVVYVSCHPGSLARDAGILV
                     HEHGYVLRRAGVMDMFPHTAHIESMAWFEHGN"
     gene            223..702
                     /locus_tag="VHE37_17140"
     CDS             223..702
                     /locus_tag="VHE37_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HVT37314.1"
                     /translation="MATEIERKFLLASDAWRAQVTRSQAMRQGYLTDAPGKSSVRVRI
                     EGERATLNIKAAVAGAARAEYEYEIPLADAAEMFRTLCGNCIEKTRHYVPQGALTWEI
                     DEFGGANAGLVVAEIELDSAEQTFERPVWMGREVTDERRYYNHHLAMHPYSTWADEH"
     gene            692..1168
                     /locus_tag="VHE37_17145"
     CDS             692..1168
                     /locus_tag="VHE37_17145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVT37315.1"
                     /translation="MNIEVDLARQLLRLHDGGRVLREYSVSTGANGIGEIHGSGCTPR
                     GRHIVRAKVGGGAAANTIFVARRPTGEMWTPELAARYPGRDWIVTRILWLSGTEIGRN
                     RLGCVDTFRRYVYIHGTPPTTRLGLPGSKGCVRMDNADMIELYDLVPAGTQVEIHE"
     gene            1152..2138
                     /gene="nagZ"
                     /locus_tag="VHE37_17150"
     CDS             1152..2138
                     /gene="nagZ"
                     /locus_tag="VHE37_17150"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HVT37316.1"
                     /translation="MKSTNKKNAPLIGPLMADVAGLELTAEDRDVLAHPLVGGVILFT
                     RNYRDGEQLSALCREILALKQPRLLLAVDHEGGRVQRFRVGFTRVPAMRTLGKLYQED
                     SAQAVAEARAHARTIGSELATHGIDLCFAPVLDRDCGTSTVIGDRAFSDDTRSIITLA
                     RAFRAGLNAAGLAATGKHFPGHGFVAADSHVELPVDRRELAVLRETELKPFEALCADG
                     IESLMMAHVRYTAVDQTPASLSHKWIGQILRGEMKYDGAIFCDDLSMGGAAAIGSMEE
                     RAQLALAAGCDMLPVCNDRPALVTLIDALKRVKPSHESSERLARLYRREVAA"
     gene            2135..2689
                     /locus_tag="VHE37_17155"
     CDS             2135..2689
                     /locus_tag="VHE37_17155"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HVT37317.1"
                     /translation="MNERLLDAQQVRESADCLHDAAAVQRAYDRLAAQIEREYAGKNP
                     LLLCVMIGGLQPTAEIVQRLAFPFELDYLHATRYRGQTSGADLHWKAHPDIAVAGRDV
                     LVIDDILDEGHTLAAILFALRGQNAASVKCAVLCEKLHARKAPDVRADFVGLTVEDRY
                     VFGCGMDYKSYWRQLPGIYAVKES"
     gene            2686..3450
                     /locus_tag="VHE37_17160"
     CDS             2686..3450
                     /locus_tag="VHE37_17160"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HVT37318.1"
                     /translation="MTTVAVIGGTGMNQWPGLEVDRTHTPDTPYGAASAAPIEGKVYG
                     IRAVFLARHGEGHKIPPHAINYRANLWALKNLGVKSVVAVAAVGGIAPWFPPGGVCVP
                     NDLIDYSYGRAHTYSDGSPGAGLDHVEFTEPYSERVRRELIHAALAARVDIGEAGVMA
                     VTQGPRLESIAEVKRLKRDGCDMIGMTQMPEAALARELGIDYACLAVSVNWAAGIGEG
                     AIHAQIHESIEAGMKRVRLILSRALPALRAQDENMG"
     gene            3612..4229
                     /locus_tag="VHE37_17165"
     CDS             3612..4229
                     /locus_tag="VHE37_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HVT37319.1"
                     /translation="MRRVLMLVALLALSACGAQPIDEKKDQPGYSGFSSRQEADQPIG
                     EGTYSRDEMLKEAQNFFGNTSQGLAQAVEKAFADYGRPNAYIAGDEGGGAFFVGLRYG
                     RGELRYKGGGSMPVYWQGPSLGLDWGGNASKVFTLVYNLHSTYQLFQRFPSVDGSLYV
                     VAGVGLNYQRSGDITLAPIRTGVGLRAGASLGYIDYTRESTINPF"
     gene            complement(4242..>5323)
                     /locus_tag="VHE37_17170"
     CDS             complement(4242..>5323)
                     /locus_tag="VHE37_17170"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HVT37320.1"
                     /translation="ARHTADAEPTALRVELSNPGNRFVGRSFLDLDITGSSAWGDEVH
                     LLWREGLNGLNGAGNDSRYHEPSIAWNRVTNYGVIGAGIHYTNFDFVFNGIPLHGDLR
                     QVEALWLYPLYADFTHHWLFELKGDRTDKKNETEADIGTPGTAGFIAAGTKEQEEVYT
                     SVEVTNTWSQKLHLLKDDSSFDAALTVRRGLSSEHQPVTPANLDYLLYRPALALKQPF
                     GERFNVSASINGQFSTDVLPEQQEWLLGGLGSMTAYLPGVAIGDVGYSARAQAEFNLD
                     VAHFELVPRAFFEYGYATFQNPSAGETSQRQTLSDVGAELAFKWGKLFEGSVAWAAPV
                     DHSGIAQSELDAQRANVYFRIAAKL"
BASE COUNT          888 a         1700 c         1863 g          872 t
ORIGIN      
        1 acaagctgct gctggatccg ccgcgttcgg gcgccgccgc ggtgctggac tacctgccgc
       61 acaaggccac gcggcgcgtg gtctatgtgt cctgccaccc cggttcgctg gcgcgcgacg
      121 ccggcatcct ggtgcacgag catggctacg tgctcaggcg cgcgggcgtg atggacatgt
      181 ttccgcacac cgcgcatatc gagagcatgg cgtggttcga acatggcaac tgagatcgaa
      241 cggaagttcc tgctggccag cgatgcctgg cgtgcgcagg tgacgcgctc gcaggcgatg
      301 cgccagggct atctcaccga tgcgccggga aagagttcgg tgcgcgtgcg catcgaaggc
      361 gagcgggcca cgctcaacat caaggccgcg gtggccggcg ccgcgcgtgc cgagtacgag
      421 tatgaaatcc cgctggccga tgccgcggag atgttccgca cgctgtgcgg caactgcatc
      481 gagaagacgc ggcattacgt gccgcagggc gcgctgacct gggagatcga cgagttcggc
      541 ggcgccaatg ccgggctggt cgtggccgag atcgagctcg attccgcaga acaaactttc
      601 gaacgcccgg tctggatggg cagggaagtg accgacgagc ggcgctatta caatcaccac
      661 ctcgccatgc atccgtattc cacctgggcc gatgaacatt gaagtcgatc tcgcgcgcca
      721 gctgctgcgg ctgcacgacg gcggccgggt gctgcgcgag tattccgtgt ccaccggcgc
      781 caacggcatc ggcgaaatcc acggctccgg ctgcacgccg cgcgggcggc acatcgtgcg
      841 cgccaaggtc ggcggcggcg cggcagcgaa taccatcttc gtcgcgcgcc ggccgaccgg
      901 cgaaatgtgg acaccggagc tggcggcgcg ctatccgggg cgcgactgga tcgtcacgcg
      961 catcctgtgg ctgtccggca ccgagatcgg gcgcaaccgg ctgggctgcg tcgatacctt
     1021 ccgccgctac gtctacatcc acggcacgcc gccaaccacg cgcctgggcc tgccgggctc
     1081 caagggctgc gtgcgcatgg acaacgccga catgatcgag ctgtacgacc tggtgcctgc
     1141 aggaacgcaa gttgaaatcc acgaataaga agaacgcgcc gctgatcggc ccgctgatgg
     1201 ccgacgttgc cggcctggaa ctgaccgccg aagaccgcga cgtactggcg catccgctgg
     1261 tcggtggcgt catcctgttc acgcgcaact accgcgacgg cgagcagctg tcggcgctgt
     1321 gccgcgaaat cctcgccctc aagcagccac gcctgctgct cgcggtggac cacgagggcg
     1381 gccgcgtgca gcgcttccgc gtcggcttca cccgcgtgcc ggccatgcgc acgctgggca
     1441 agctatacca ggaggattct gcgcaggcgg tggccgaggc gcgcgcacac gcgcgcacca
     1501 taggcagcga gctggccacg catggcatcg acctgtgttt cgcgccggtg ctggaccgcg
     1561 actgcggcac cagcacggtg atcggtgacc gcgccttctc cgacgacacg cgcagcatca
     1621 tcacgctggc ccgcgcgttc cgcgccggcc tgaatgccgc aggcctggcg gccaccggca
     1681 agcattttcc cggccatggt ttcgtcgccg ccgattcgca cgtggagctg ccggtggacc
     1741 ggcgcgaact ggcggtgctg cgcgaaaccg agctgaagcc attcgaggcg ctgtgcgccg
     1801 acggcatcga gtcgctgatg atggcgcacg tgcgctacac cgcggtggac cagacgccgg
     1861 catcgctgtc gcacaagtgg atcggccaga tcctgcgcgg cgaaatgaaa tatgacggcg
     1921 ctatcttttg tgacgacctg tcgatgggcg gcgccgccgc catcggcagc atggaagagc
     1981 gcgcgcagct ggcgctggcc gcgggctgcg acatgctgcc ggtgtgcaac gaccgcccgg
     2041 cgctggtcac gctgatcgat gcgctgaaaa gagtgaagcc ctcgcatgag agcagcgagc
     2101 ggctggcgcg cctgtaccgc cgcgaggttg ccgcatgaat gaacgtctgc tggatgcaca
     2161 gcaggtgcgc gagtccgccg actgcctgca tgacgcggcg gcggtgcagc gcgcctacga
     2221 tcgcctggcc gcgcagatcg agcgcgagta cgcggggaaa aacccgctgc tgctgtgcgt
     2281 gatgatcggc ggactgcagc cgaccgcgga gatcgtccag cgcctggcat ttccgttcga
     2341 actcgactac ctgcacgcca cgcgttaccg cggccagacc agcggcgccg acctgcactg
     2401 gaaggcgcac ccggacatcg ccgtggccgg gcgcgacgtg ctggtgatcg acgacattct
     2461 cgacgagggt cacacgctgg cggcgatcct cttcgcgctg cgcgggcaga acgctgcctc
     2521 ggtgaaatgc gcagtgctgt gcgagaagct gcatgcgcgc aaggcgccgg acgtgcgcgc
     2581 cgatttcgtc gggctcaccg tcgaggatcg ctacgtcttc ggttgcggca tggattacaa
     2641 gagctactgg cggcagttgc cgggaatcta cgcggtgaag gaatcatgac gacggtcgcg
     2701 gtgattggcg gcactggcat gaaccagtgg ccgggcctgg aagtggacag gacgcacacg
     2761 ccggatacgc cgtacggcgc ggcgagcgca gcgcccatcg aggggaaggt ctacggcatc
     2821 cgcgcggtct tcctcgcgcg tcacggtgag ggccacaaga tcccgccgca cgccatcaac
     2881 taccgcgcca acctgtgggc gctgaagaac ctcggcgtga agagcgtggt cgcagtggcg
     2941 gcggtcggcg gcattgcgcc gtggtttccg cccgggggcg tgtgcgtgcc caatgacctg
     3001 atcgattact cgtatggacg tgcgcacacc tattccgacg gctcgcccgg cgccgggctg
     3061 gaccacgttg agttcaccga gccgtacagc gagcgcgttc ggcgtgagct gatccacgcc
     3121 gcgttggccg cgcgcgtgga catcggcgag gctggcgtga tggcggtgac gcaggggccg
     3181 cggctggagt cgattgcgga agtgaagcgt ctcaagcgcg acggctgcga catgatcggc
     3241 atgacgcaga tgccggaggc ggcgctggcg cgcgaactgg gcatcgacta cgcctgcctc
     3301 gcggtgagcg tgaactgggc ggcgggcatc ggcgaaggcg cgatccacgc gcagatccac
     3361 gaatcgattg aagccggcat gaagcgcgtg cggctgattc tttcccgtgc gctgccggcg
     3421 ctgcgtgcac aggacgagaa catgggctga aacgcagcgg gggaggtcaa ccgcatccgg
     3481 gttttggctg taataaaata tagtgcaaaa gtaaaatcgt tcgatataaa ataacgacga
     3541 aaaccggggt gcggtgagcg aacgccggcc gcatttcggg gtcattcaag gacacgattg
     3601 aaggtgaact catgcgccgc gtattgatgc ttgttgcgct tctcgcgctg tcggcttgcg
     3661 gtgcgcagcc gatcgacgag aagaaagacc agcccggcta ttccgggttc tccagccgcc
     3721 aggaggcgga ccagccgatc ggcgagggca cttattcgcg cgacgagatg ctgaaggagg
     3781 cgcagaactt cttcggcaac accagccagg gcctggcgca ggccgtcgag aaggccttcg
     3841 ccgactacgg ccgccccaat gcctacatcg ccggcgatga aggcggcggc gcgttcttcg
     3901 tcggcctgcg ctacggccgt ggcgagctgc gctacaaggg cggcggcagc atgccggtgt
     3961 actggcaggg cccgagcctg ggcctggact ggggcggcaa cgcgtcgaag gtgttcacgc
     4021 tggtctacaa cctgcacagc acctaccagc tgttccagcg cttcccgtcg gtggacggct
     4081 cgctctacgt ggtcgccggc gtcggcctca attaccagcg cagcggcgac atcacgctgg
     4141 cgccgatccg caccggcgtg gggctgcgcg ccggcgcgag cctgggctac atcgactaca
     4201 cgcgggaatc gacgatcaac ccgttctgaa gcggcggcgg tctacagttt tgccgcgatg
     4261 cggaagtaga cgttggcgcg ctgcgcatcg agttcgctct gcgcgatgcc gctgtggtcg
     4321 accggcgcgg cccaggccac cgacccctcg aacagcttgc cccacttgaa cgccagttct
     4381 gcgccgacgt cggacagcgt ctgccgctgg ctggtttccc cggccgacgg gttctggaac
     4441 gtggcgtagc cgtactcgaa gaaggcgcgc ggcaccagtt cgaaatgcgc gacgtcgagg
     4501 ttgaactcgg cctgcgcacg tgcggagtag ccgacgtcgc cgatggccac gccgggcagg
     4561 taggccgtca tgctgcccag gccgccgagc agccattcct gctgctctgg cagcacgtcg
     4621 gtgctgaact gcccgttgat gctggcgctg acgttgaaac gctcgccgaa cggctgcttg
     4681 agcgcgagcg ccgggcggta cagcaggtaa tccaggttgg ccggcgtcac cggctggtgc
     4741 tcgctggaca ggccgcggcg cacggtgagc gcggcgtcga agctggaatc atccttgagc
     4801 aggtgcagtt tctgcgacca ggtgttggtc acttccacgc tggtgtagac ctcttcctgt
     4861 tccttggtgc cggccgcgat gaaacccgcg gtgccgggcg tgccgatatc ggcttccgtc
     4921 tcgttcttct tgtcggtgcg gtcgcccttg agttcgaaca gccagtgatg ggtgaagtcc
     4981 gcgtacagcg ggtacagcca caaggcttcc acctggcgca ggtcgccgtg cagcgggatg
     5041 ccgttgaaga cgaaatcgaa gttggtgtaa tggatgccgg cgccgatcac gccgtaattg
     5101 gtcacgcggt tccaggcgat gctcggctcg tggtagcggc tgtcgttgcc cgcgccgttg
     5161 aggccgttga gtccctcgcg ccacagcagg tgcacttcgt cgccccaggc gctgctgccg
     5221 gtgatgtcga ggtcgaggaa gctgcggccg acgaagcggt tgcccgggtt gctcagctcc
     5281 acgcgcagcg cggtgggctc ggcgtcagcg gtgtggcgcg ctt
//
LOCUS       DASZZC010000798         5220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_639293,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000798 DASZZC010000000
VERSION     DASZZC010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639293"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VHE37_17175"
     CDS             <1..271
                     /locus_tag="VHE37_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HVT37321.1"
                     /translation="LLGTRLETYNLLSQAARDSGNTAEASYQMANYLFARGDAGGALA
                     SLDAGLRAGGLSSQDRARLIARRQEVRESLPRDWRPSPNQRLGGI"
     gene            complement(281..1264)
                     /locus_tag="VHE37_17180"
     CDS             complement(281..1264)
                     /locus_tag="VHE37_17180"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVT37322.1"
                     /translation="MPKTAFVTGATGFVGLNLVDRLVDQGWHVVAMHRRSSDLTLLSR
                     FKVERVIGDVVDKSSLDGIIPRRVDCVFHVAGNTSRWSRTHAEQLKVNVKGTRNMVRA
                     ALQAGVKCFVHTSSIVAYGLHGGVITEETPTRGTAAKLNYIRSKALAEREVRKGIALG
                     LKAVILNPSNIMGAYDTSSWSRMFRLVKSGRMPAVPPGGGSFCHAREAALAHIAAAER
                     GRLGQNYLLGGTQSTYLGLAREMSRMLGVRRRFVSMPPDLLNAYARVEEWVAPLFGRE
                     PDVTCDAIALLSQNSYCSSRKAEQELGYRNVPLEQMLRDCYGWMQSQRMFG"
     gene            complement(1328..2278)
                     /locus_tag="VHE37_17185"
     CDS             complement(1328..2278)
                     /locus_tag="VHE37_17185"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37323.1"
                     /translation="MTLAGLYRRRGEVDLALRLHEDLLARSDLSGVQREEARFELAQD
                     FLKAGMLDRAEKLFEELAAEGVRVVAALEQIVAIFEQGRDWKHAIDAARRLEAAKGES
                     RRSLVAQYQCELAEEAAREGRNDDALKLIKRALDDDRTCVRAWLALARLERGAGDMAG
                     AIAAYRHAFDQDARFLPEVIGSLGECYAQAGDARGYVDFLAHAAESGAPALAAAAQAR
                     LMQQEGLDPTESLARALNAHPSRALLVDFLELLQRRPEAIAAGLAQPAASLAVAVRKL
                     VDAAPKYRCGNCGFTARSLFWQCPTCRQWASIAPIEDKLG"
     gene            complement(2481..2750)
                     /locus_tag="VHE37_17190"
     CDS             complement(2481..2750)
                     /locus_tag="VHE37_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HVT37324.1"
                     /translation="MVIVVSIFLCGGIALGYFNSEKVSFNFLLGTAQLPLIVLLAEVF
                     IAAVALTLLVVAIRIFALHAETRRLRKQLNDAESELKNLRNLPYA"
     gene            complement(2777..3082)
                     /locus_tag="VHE37_17195"
     CDS             complement(2777..3082)
                     /locus_tag="VHE37_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HVT37325.1"
                     /translation="MTKSELIERLSTQQTHLMQKDVELAVKLVLDHISTALSRDDRVE
                     IRGFGSFSLHHRPARVGRNPKTGEPVQIPPKSVPHFKPGKEMRERVNAAAQAAQKPL"
     gene            complement(3229..4896)
                     /gene="rpsA"
                     /locus_tag="VHE37_17200"
     CDS             complement(3229..4896)
                     /gene="rpsA"
                     /locus_tag="VHE37_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HVT37326.1"
                     /translation="MSESFAELFEASLKGGQSMQSGTIVNAVVLAVKPDVVIVDAGLK
                     SEGVIPVSEFTIDGEVKVAVGDQVEVALETVEDGFGETRFSKEKAERIKTWDRLAKAH
                     EGKETVIGIITGKVKGGFTVDIGTVRAFLPGSLVDVRPVRDTSYLEGKPLEFKVIKLD
                     KLRNNVVVSRREVLEAEYSAERDSLLANLQEGVVLKGVVKNLVEYGAFVDLGGIDGLL
                     HITDMTWKRVKDPAEVVQVGAEIEVKVLKYDRERRRVSLGLKQLGADPWSEIERRYPE
                     KTRLFGKVTNITDYGAFVEIEPGVEGLVHVSEMDWTNKNINPSKAVSVGDEVEVMILD
                     IDGERRRISLGMKQCKPNPWEEFAQNHQKGDKVHGAIKSITDFGIFIGLEGGIDGLVH
                     LSDLDWNEPGETAVRKYQKGQELDAVVLAIDGARERISLGVKQVQQDPLAQFIAEHTK
                     GSIVKGTVRTVDARGAIIDLAPGVEGYIKANDLSRDRVEDATKVLSVGGTLEARFIGV
                     DRKNRVVSLSVREKEIQEEQEAMAEYSRNASTGKTSLGDILKEQIGN"
     gene            complement(4983..>5220)
                     /locus_tag="VHE37_17205"
     CDS             complement(4983..>5220)
                     /locus_tag="VHE37_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HVT37327.1"
                     /translation="ASAEERARRRHKQLMGMGQTANLDSLRIEILQRDERDRTRATAP
                     LVPARDAVTIDTTSLPPADVLAKVDELLQSRGLL"
BASE COUNT          886 a         1724 c         1604 g         1006 t
ORIGIN      
        1 gctgctgggc acgcggctgg aaacctacaa cctgctgtcg caggcggcgc gcgactccgg
       61 caacactgcc gaggcgtcgt accagatggc caactacctg tttgcccgtg gcgacgccgg
      121 cggtgcgctg gcgtcgctcg atgccggcct gcgcgccggc ggcctgtcgt cgcaggaccg
      181 cgcccggctc attgcgcgcc gccaggaagt gcgcgaatcg ctgccgcgcg actggcggcc
      241 atctcccaat cagcgcctcg gcggcatctg aatcgcaact tcagccgaac atgcgctgtg
      301 actgcatcca gccgtagcag tcacgcagca tctgctccag cggcacgttg cggtagccca
      361 gctcctgctc ggccttgcgg ctgctgcagt agctgttctg cgacagcagc gcgatggcgt
      421 cgcaggtcac gtcgggctcg cgcccgaaca atggcgccac ccattcctcc acccgtgcat
      481 acgcgttgag caggtccggc ggcatggaca cgaagcggcg gcgcacgccg agcatgcggc
      541 tcatttcgcg cgccagtccc agataggtgc tttgcgtgcc gcccagcagg tagttctgcc
      601 ccagccggcc gcgctcggcc gcggcgatat gcgccagcgc cgcctcgcgc gcgtggcaga
      661 agctgccgcc gcccggcggt acagcgggca tgcgtccgct cttcaccagc cggaacatgc
      721 gcgaccagct gctggtgtcg taggcgccca tgatgttcga cgggttgaga atgaccgcct
      781 tgagcccaag cgcgatgcct ttgcgcacct cgcgttccgc cagcgccttg ctgcggatgt
      841 agttgagctt cgccgccgtg ccgcgtgtcg gcgtctcctc ggtgatcacg ccgccatgca
      901 ggccgtaggc gacgatgctg gaggtgtgta cgaagcattt cacgccggcc tgcaaagcag
      961 cgcgcaccat gttgcgcgtg cccttcacgt ttaccttgag ctgttccgcg tgggtgcgcg
     1021 accaccggct ggtgttgccg gcgacatgga acacgcaatc gacccgcctg ggaatgatgc
     1081 cgtcgaggct ggacttgtcg actacgtcgc cgatcacccg ctccaccttg aagcggctga
     1141 gcagggtcag gtcggaactg cggcggtgca tggcgacgac gtgccagccc tggtcgacca
     1201 gccggtcgac caggttgagg ccgacgaagc cggtagcgcc ggtgacgaac gcggttttcg
     1261 gcacggcggc ggctcccggg agattgccgg caagaatagg tcttgcgcag cgctttgcaa
     1321 cctcgagcta gccgagcttg tcctcgatgg gcgcgatgct ggcccactgc cgacaggtgg
     1381 ggcattgcca gaacaggctg cgtgcggtga agccgcaatt gccgcaacgg tatttcggcg
     1441 ctgcgtcgac cagcttgcgc acggcaaccg ccaggctggc cgccggctgc gccagccccg
     1501 cggcgatcgc ctccggcctg cgctgcagta gttcgagaaa atccaccagc agggcgcggc
     1561 tcggatgagc attgagcgcc ctggccaggc tctccgtcgg atcaagtcct tcctgctgca
     1621 tcaggcgcgc ctgcgccgca gcagccaggg cgggcgcacc gctttcggcg gcatgcgcga
     1681 gaaaatcgac ataaccacgc gcatcccctg cctgcgcata gcattcgccg agtgagccga
     1741 tgacttcggg cagaaagcgt gcatcctggt cgaacgcatg ccggtatgcg gcgatggcgc
     1801 ctgccatgtc tccggcgcca cgctccagcc gcgccaaggc aagccatgcg cgcacgcagg
     1861 tgcggtcgtc gtcgagcgcg cgcttgatca gcttgagcgc gtcgtcgttg cgcccctcgc
     1921 gcgcagcttc ctcggccagt tcgcattgat actgggccac caggctgcgc ctggattcgc
     1981 ccttggcggc ttccaggcgg cgggcggcat cgatggcgtg cttccagtcg cggccctgct
     2041 caaagatggc gacgatctgt tcgagtgcgg caaccacgcg cacgccctcg gccgccagtt
     2101 cctcgaacaa tttctcggca cgatccagca tgccggcctt gagaaaatcc tgcgcgagtt
     2161 cgaagcgcgc ttcctcgcgt tgcacgccgc tcaggtcgct gcgggccagc agatcctcat
     2221 gcaggcgcag cgccagatcc acttcgccgc gcctgcggta caggccagcc agcgtcaact
     2281 gcagatcgat cgcgttttca gtccttccgg gacgctgcgt cagtgctgca atggcctggt
     2341 cggcacgatc gcccgtcgcc ggcgaggctg cgctgtgggg cgcaggatcg ggaccctgcc
     2401 cctcggcccg gttatcgcgc cgggcgaaac tccatcccag tgccgctccc agcggaagca
     2461 gcagccaggt gagcaaccct tcaagcatag ggaagattgc gcaggttctt caattcgctt
     2521 tcggcgtcat tgagttgctt gcgcagccgc cgcgtttcgg cgtgcagcgc gaagatgcgg
     2581 atggccacca ccagcagggt caaggccacg gcagcaatga acacttccgc cagcagcacg
     2641 atcagcggca gctgggccgt tcccagcagg aagttgaacg agaccttttc cgaattgaaa
     2701 tagcccagcg caattccgcc gcaaaggaat atggatacaa caataaccaa tataatccta
     2761 tacatggatt atattattaa agtggtttct gcgcagcctg cgcggcagca ttgacccgct
     2821 cgcgcatttc cttgcccggc ttgaaatgcg gcacgctctt aggcggaatc tgcaccggtt
     2881 cgccggtctt gggattccgt ccgacgcggg ccggccggtg atgcagtgaa aagctgccga
     2941 aaccgcggat ctccacgcga tcgtcgcgtg acagtgcggt gctgatgtgg tccagtacca
     3001 gtttgaccgc gagttccacg tccttctgca tcaggtgggt ctgctgcgtg gacaggcgtt
     3061 cgatcaattc tgatttggtc atgtcgtttt cccgcccgca ccgaccggcg ggttgattgc
     3121 cggcgacgat aaataaaaag cgagggaggc gcaagcgctt cccccgccgt tttttctcgt
     3181 atttaccgag agatgcggac gaatctgaaa gtccgcatca gcacagcctc agttgccgat
     3241 ctgctccttg aggatgtcgc ccaggctggt cttgccggtg gaagcattgc ggctgtattc
     3301 cgccatggct tcctgttctt cctgaatttc cttctcacgg accgaaaggc tgaccacacg
     3361 attcttgcgg tcgacaccga tgaagcgggc ttccagtgtg ccgccgaccg acagaacctt
     3421 ggtagcgtct tcgacgcggt cacgcgacag atcgttggcc ttgatatagc cctccacgcc
     3481 cggcgccagg tcgatgattg caccacgcgc atcgaccgtg cgcacggtac ccttcacgat
     3541 gctgcccttg gtgtgttcgg cgatgaactg cgccagcggg tcctgctgca cctgcttgac
     3601 gcccagcgag atgcgctcgc gcgcgccgtc gatggccagc accacggcat ccagttcctg
     3661 gcccttctgg tacttgcgca ccgccgtttc gcccggctcg ttccagtcca ggtcggacag
     3721 gtgcaccaag ccgtcgatgc cgccttccag gccgatgaag atgccgaagt cggtgatcga
     3781 cttgatggcg ccatgcacct tgtcgccctt ctggtggttc tgcgcaaact cttcccaggg
     3841 attgggcttg cactgcttca tgcccaggct gatgcggcgg cgctcgccgt cgatatcgag
     3901 gatcatcacc tcgacttcat cgccgaccga cacggccttg ctcgggttga tgttcttgtt
     3961 ggtccagtcc atttccgaaa cgtggaccag gccttcgaca cccggctcga tctcgacgaa
     4021 tgcgccgtag tcggtgatgt tggtgacctt gccgaacaga cgggtcttct cggggtagcg
     4081 gcgctcgatc tcgctccagg gatcggcacc cagctgcttg aggcccaggg agacgcggcg
     4141 gcgctcgcgg tcgtacttga gcaccttgac ctcgatttcg gcgccaacct gcaccacttc
     4201 cgcaggatcc ttgacgcgct tccaggtcat atcggtgatg tgcagcaggc cgtcgatgcc
     4261 gccgaggtcg acgaacgcgc cgtactcgac caggttcttg accacgccct tgagcaccac
     4321 gccttcctgc aggttggcga gcaacgaatc gcgctcggcg ctgtactcgg cttcgagcac
     4381 ttcacgacgc gacaccacga cgttgttgcg cagcttgtcg agcttgatga ccttgaactc
     4441 gagcggcttg ccttcgaggt aagaggtatc gcgtaccggg cgcacatcga ccagcgagcc
     4501 aggcaggaac gcgcggacgg tgccgatgtc cacggtgaag ccgcccttga ccttgccggt
     4561 gatgatgccg atgacggttt ccttgccctc gtgcgccttg gccaggcgat cccaggtctt
     4621 gatccgctcg gccttctctt tcgagaagcg cgtttcgccg aaaccgtcct cgacggtttc
     4681 cagcgcgact tccacttggt cgccgacagc gaccttgact tcgccgtcga tggtgaattc
     4741 ggaaacaggg atgacgccct cggacttcag tccggcgtca acgatgacca cgtcaggctt
     4801 gaccgcaaga accaccgcat tgacgatggt gcccgactgc atcgactggc cgcccttgag
     4861 gctggcctcg aacaactccg caaaactctc gctcatacta agactcgtta agttgaacaa
     4921 ccaccacgat ccttcgggtg ggcctgtctg atgaataaga ggccgcaaat ccttttcggc
     4981 cctcagagta aaccgcgaga ttggagcagt tcgtccactt tcgccagcac gtcggcaggc
     5041 ggcagagaag tcgtgtcaat cgtcacagca tcccgcgcgg gaaccagcgg cgccgtggcg
     5101 cgtgtccggt cccgttcgtc gcgttgcaaa atctcaatgc gaagactgtc aaggttagca
     5161 gtttgaccca ttcccatcaa ctgcttatga cgcctgcgag cccgttcctc ggcactcgct
//
LOCUS       DASZZC010000799         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_446182,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000799 DASZZC010000000
VERSION     DASZZC010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446182"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="VHE37_17210"
     CDS             complement(<1..1437)
                     /locus_tag="VHE37_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HVT37328.1"
                     /translation="MSRLEKMVEGISNLLIAWRHALSVLFAALTLFFCWSATHVRLDP
                     GFLKLIPIEHPYMKTMMEYIDFSGANTLLVNLRWKGEGDIYNAPFMDAMAKATDDVFF
                     IPGIDRTHVSSIFTPDTYYIEITEDGFKGEPVVPARFSGTPEQLAKVRHNVSLTGEIG
                     LIVSNDQKSALIRADLQEVDSNNPAKAEVFYRQVMSRLADIRGRVESPVKYTYKLKAD
                     HAPFKAGEVIAEGYVDYGFMQKFETFYKLPPDGGDAIAIDGSELAVEQGANPDYNKDI
                     EVNIIGFAQLLSDVIHGLIGVFAFFGLAFVITIVLLFQYARSVKLTIVALVVALLPVL
                     WLIGILPLIGFGIDPMSILVPFLIFSIGVSHAVQMTNAWRLEVTGGASAIEASRASFR
                     KLFIPGAVALLTNALGFGVIMLIHIPIVHELGITACLGVLLMIATNKMILPIILTHVK
                     LEESCKRYSARPPSPSMARVWGAIAKCAE"
     gene            complement(1434..2486)
                     /locus_tag="VHE37_17215"
     CDS             complement(1434..2486)
                     /locus_tag="VHE37_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37329.1"
                     /translation="MPAYVAVAVVIAAAVYAFSPRPQPAFAPTAIHPDRILVNGLAQQ
                     GSRIVAVGELGRVLLADQPDGPWHEAEVQPQRGSTLTQVKFIADHALLAVGHDGWIIG
                     STDNGEHWKELAFTAPPPDEAPAPNPSMPEGAPPLPDIGGGMGGPPPANPLMGVAGPF
                     DGKLFVFGGFGELRSSTDGGKSWNAISDAAIGDKHVNAMAQLADGSLLAAGERGLLVR
                     STDLGATWKALPQIYNGSFFGLLTLPDHKIVAYGMRGNVYVSADNGASWKKSEVPDSI
                     SLFGGAVDADGTPVLVGEGDSVLRSTDGGATFSVATSGKRERLVAVLPQKGGWLAAGE
                     GGIMLLHPAAAAGEHS"
     gene            complement(2677..3879)
                     /locus_tag="VHE37_17220"
     CDS             complement(2677..3879)
                     /locus_tag="VHE37_17220"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVT37330.1"
                     /translation="MTEAVIVSVARTAIGKAYRGAFNDTEAPVLGGHVVRHAVQRAGI
                     DPKEVDDVIIGAAAQQGTQAYNLGRLCAYTAGLPDTVPGMTLDRQCSSGLQSIAIAAK
                     NIMCGEMDIVVAGGLESISLTQNKHKNSYRFVSEAVIAAEPTAYIPMIETAEIVAERY
                     KISRAQQDEYALQSQQRTAAAQVAKKFDDEIVPLTAQKQLYDKEGNPTTKETVTLTKD
                     EGNRADTSAESLAALKPVWKDGRWVKAGQHITAGNASQLSDGAAVTLLMSRDAAKARG
                     LKPLGIYKGYAVAGCKPDEMGIGPVFAVPRLLAKHGLKVSDVGLWELNEAFAVQVIYC
                     RDQLGIPNDRLNVNGGAISIGHPFGMSGARMVGHALIEGRRRGVKHVVVTMCIGGGMG
                     AAGLFEIP"
     gene            complement(3890..4345)
                     /locus_tag="VHE37_17225"
     CDS             complement(3890..4345)
                     /locus_tag="VHE37_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVT37331.1"
                     /translation="MRPVFDSAEAILAAVGKPLGTSEWVTITQERVNLFADATDDHQW
                     IHVDPVKAKAGPFGAPIAHGYLTLSLVSRFLPEIVDVKIRMGVNYGTDKVRFPSAVKV
                     GSRIRGRGDLISAEKTKDGAVQAVIRVTVEIEGADKPACIADAISRYYF"
     gene            complement(4353..>4808)
                     /locus_tag="VHE37_17230"
     CDS             complement(4353..>4808)
                     /locus_tag="VHE37_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT37332.1"
                     /translation="KRVVDMTDEEWTRVLDVTLTGTFRMTRAMLKRMQPRGRGAIVNN
                     ASVLGWRAQKEQAHYAAAKAGVMALTRCSALEAAEFGIRINAVSPSIAFHEHLKKTAP
                     AELLKDLASREAFGRAAEVWEIANVIVFLASDYASYLVGEVISASSQRS"
BASE COUNT          761 a         1620 c         1578 g          849 t
ORIGIN      
        1 ttcggcgcac ttggcaatcg cgccccacac ccgcgccatc gacggcgacg gcggccgcgc
       61 cgagtagcgc ttgcacgact cttccagctt cacgtgcgtc aggatgatcg gcaggatcat
      121 cttgttggtg gcgatcatca gcagcacgcc gagacacgcg gtgatgccga gctcgtgcac
      181 gatgggaatg tggatcagca tgatcacgcc gaagcccagc gcgttggtca gcagcgccac
      241 cgcgccggga atgaacagct tgcggaacga cgcgcgcgag gcctcgatgg cgctggcgcc
      301 gccggtcact tccaggcgcc aggcgttggt catctgcacc gcgtgcgaca cgccgatgga
      361 gaagatcagg aacggcacca ggatcgacat cgggtcgatg ccgaagccga tcagcggcag
      421 aattccgatc agccacagca ccggcagcag cgccaccacc agtgccacga tggtcagctt
      481 gaccgaccgc gcatactgga acagcagcac gatggtgatg acgaaggcca gcccgaagaa
      541 cgcgaacacg ccgatcaggc cgtggatcac gtcggacagc agctgggcga agccgatgat
      601 gttgacctcg atgtccttgt tgtagtccgg gttggcgccc tgctccaccg ccagctcgct
      661 gccgtcgatg gcgatggcgt cgccgccgtc cggcggcagc ttgtagaagg tctcgaactt
      721 ctgcatgaag ccgtagtcga cgtaaccctc ggcgatgacc tcgcccgcct tgaacggcgc
      781 gtggtcggcc ttgagcttgt aggtgtactt caccgggctc tcgacgcggc ctcggatgtc
      841 ggccaggcgc gacatcacct ggcggtagaa cacctcggcc ttggccgggt tgttgctgtc
      901 cacttcctgc aggtcggcgc ggatcagcgc gctcttctgg tcgttggaca cgatcaggcc
      961 gatctccccg gtgagcgaca cgttgtggcg caccttggcc agctgctcgg gcgtgccgga
     1021 gaagcgcgcc ggcaccaccg gctcgccctt gaaaccgtcc tcggtgatct cgatgtaata
     1081 cgtgtccggc gtgaagatcg acgacacgtg cgtgcggtcg atgccgggaa tgaagaacac
     1141 gtcgtcggtg gccttggcca tcgcgtccat gaacggcgcg ttgtagatgt cgccttcgcc
     1201 cttccagcgc aggttgacca gcagcgtatt ggcgccggag aagtcgatgt actccatcat
     1261 cgtcttcatg tacggatgct cgatcgggat cagcttgagg aatcccgggt cgagccgcac
     1321 gtgcgtggcg ctccagcaga agaacagcgt caacgcggca aacagcaccg acagcgcatg
     1381 gcgccaggcg atcagcaggt tggagatgcc ctcgaccatc ttttccagcc ggctcatgaa
     1441 tgttctcccg ccgcggccgc tgggtgcagc agcatgatcc cgccctcgcc ggcggccagc
     1501 cagccgccct tctgcggcag caccgccacc aggcgctcgc gcttgccgct ggttgctacg
     1561 gaaaacgtag cgccgccatc ggtggagcgc agcacgctgt cgccctcgcc caccagcacc
     1621 ggcgtgccgt cggcatccac cgcgccgccg aacagcgaga tggaatccgg cacttcactc
     1681 ttcttccagc tggcaccgtt atcggcgctc acatagacgt tgccgcgcat gccgtaggcc
     1741 acgatcttgt ggtcgggcag cgtgagcagg ccgaaaaacg agccgttgta gatctgcggc
     1801 agcgccttcc aggtggcgcc caggtcggtg gagcgcacca gcaatccgcg ctcgcccgcg
     1861 gccagcagcg agccgtcggc cagctgcgcc atggcattga cgtgcttgtc gccgatggcc
     1921 gcatcgctga tcgcgttcca gctcttgccg ccatcggtcg atgagcgcag ctcgccgaag
     1981 ccgccgaaca cgaacagctt gccgtcgaac ggaccggcca cgcccatcag cgggttggcc
     2041 ggcggcggcc cgcccatgcc gccaccaatg tcgggcagcg gcggtgcgcc ttccggcatc
     2101 gagggattgg gcgccggcgc ctcgtccggc ggcggtgcgg tgaaggccag ttccttccag
     2161 tgctcaccgt tgtcggtgct gccgatgatc cagccgtcgt gaccgacggc aagcaacgcg
     2221 tgatcggcga tgaacttcac ctgcgtcagc gtggagccgc gctgcggctg cacctccgcc
     2281 tcgtgccagg gtccatcggg ctggtcggcc agcagcacgc ggccgagctc gccgaccgca
     2341 acgatgcgcg agccctgctg cgcgagacca ttgaccagga tgcgatccgg gtggatcgcg
     2401 gtgggcgcaa aggccggctg cggccgcggt gagaacgcgt acacggcggc cgcgatcacc
     2461 accgcgaccg cgacatacgc gggcatgctg ttatgccgag ctgcatccgt catcttcttt
     2521 tcctccggcc gcggtctttg ccgtggcccg cttgatcttg tcattcccgc cccgacaccc
     2581 gccctcgggg gaggctgcgg cgggaatcca gtttgtcttg tatcccgccg caaaactgga
     2641 tgccggccta cgccggcatg acggattgtt ttttctctat gggatctcga acaatcccgc
     2701 ggcccccatg ccgccgccga tgcacatggt caccaccaca tgcttcaccc cgcgccggcg
     2761 gccttcgatc agcgcgtggc cgaccatgcg cgcgccggac atgccgaacg gatggccgat
     2821 gctgatggcg ccgccgttga cgttgagccg gtcattggga atgcccagct gatcgcggca
     2881 gtagatcacc tgcacggcga aggcttcgtt cagttcccag agtccgacat cggaaacttt
     2941 cagcccgtgc tttgccagca gcctgggcac cgcgaacacc gggccgatgc ccatctcgtc
     3001 gggtttgcag ccggccaccg cgtaaccctt gtagatgccc agcggcttca gcccgcgcgc
     3061 cttggccgca tcgcgcgaca tcagcagcgt caccgccgcg ccgtcggaca gctgcgaggc
     3121 gttgccggcg gtgatgtgct ggcccgcctt gacccagcgg ccgtccttcc acaccggctt
     3181 gagcgcggcc agcgactcgg cgctggtgtc ggcacgattg ccttcgtcct tcgtcagcgt
     3241 gacggtttcc ttggtcgtcg ggttgccttc cttgtcgtac agctgcttct gcgcggtgag
     3301 cggcacgatc tcatcgtcga acttcttcgc cacctgcgcc gcggcggtgc gctgctggct
     3361 ctgcagcgcg tactcgtcct gctgcgcgcg gctgatcttg tagcgctcgg ccacgatctc
     3421 cgccgtctcg atcatcggga tgtaggcggt gggctcggcg gcgatcactg cttccgacac
     3481 gaaccgatag gaattcttgt gcttgttctg cgtgagcgaa atcgactcca ggccgccggc
     3541 gaccacgatg tccatctcgc cgcacatgat gttcttggcg gcgatggcga tggactgcag
     3601 gcccgacgag cattgccggt ccagcgtcat gcccggcacc gtgtcgggca ggccggcggt
     3661 gtaggcgcac aggcgcccga ggttgtaggc ctgcgtgccc tgctgcgcgg cggcgccgat
     3721 gatcacgtca tcgacttcct tcggatcgat gccggcgcgc tgcaccgcgt ggcgcaccac
     3781 gtggccgccg agcaccggcg cctcggtgtc gttgaacgcg ccgcggtagg ccttgccgat
     3841 ggcggtgcgg gcaacggaga cgatgacggc ttcggtcatg gcaggctctt cagaagtaat
     3901 agcgggaaat ggcatcggcg atgcacgccg gcttgtcggc gccttcaatc tcgacggtga
     3961 cgcggatgac ggcctgcacc gcgccgtcct tggtcttctc cgcgctgatc aggtcgccgc
     4021 ggccgcggat gcggctgccg accttcaccg ccgacgggaa gcgcaccttg tcggtgccgt
     4081 agttcacgcc catgcggatc ttcacgtcga cgatctccgg caggaagcgc gacaccagcg
     4141 acagggtgag atagccatgg gcgatcggcg cgccgaacgg cccggccttg gccttcaccg
     4201 ggtccacgtg gatccactgg tgatcgtcgg tggcgtcggc aaaaagattg acgcgctcct
     4261 gcgtgatggt cacccactcg ctggtgccca gcggcttgcc aactgcggcg aggatggctt
     4321 cggcggaatc gaatacgggg cgcatggcag gactagctcc tctggctcga cgcggaaatt
     4381 acctcgccga ccaggtaact cgcatagtcc gaagcgagga agacgatgac gttggcgatt
     4441 tcccacacct cggcggcgcg gccaaaggcc tcgcggctgg ccaggtcctt gagcagttcc
     4501 gccggcgcgg ttttcttcag gtgctcgtgg aaggcgatgg acggcgacac cgcgttgatg
     4561 cggatgccga actcggcggc ttcgagcgcc gagcagcggg tcagcgccat cacgccggcc
     4621 ttggccgccg cgtagtgcgc ctgctccttc tgcgcgcgcc agccgagcac cgaggcgttg
     4681 ttgacgatgg cgccgcgccc gcgcggctgc atgcgcttga gcatggcgcg cgtcatgcgg
     4741 aaggtgccgg tgagcgtgac atccaggacc cgtgtccatt cctcgtcggt catgtcgacc
     4801 acgcgctt
//
LOCUS       DASZZC010000800         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_890198,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000800 DASZZC010000000
VERSION     DASZZC010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890198"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VHE37_17235"
     CDS             complement(<1..930)
                     /locus_tag="VHE37_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl carboxypeptidase II"
                     /protein_id="HVT37333.1"
                     /translation="MHPIRNFLLGATAVTALGLSAGAAPAPAPAPTAAASTNPFAQPS
                     TLPLQAPEFDKIKDSDYEPAFEQGMKEHLAEIERIANDPAAPTFENTIVAMEKSGRML
                     DRVSTTFFNIVSANTNPALDKVQAKVAPELAAHQDAIFLNPKLFQRVKALYDKRDQLQ
                     LDPESAQLLKIYYLQFEHAGANLSDADKEKLRAINKEDATLSTAFQQKLLAATKAGAL
                     VIDKKSDLDGLSDQEIAAAAKAAQDRNLAGKWVIPLQNTTQQPLLVSLKNRAVRQALF
                     EHSWTRAEKGDANDTRDTISRLAQIRAEKAKLLG"
     gene            complement(1058..1441)
                     /locus_tag="VHE37_17240"
     CDS             complement(1058..1441)
                     /locus_tag="VHE37_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37334.1"
                     /translation="MLKLLFAAFQWLPVIFDWAIAPAALVLLGLSFFTGTRRIAAAGF
                     LVAAAISAALLSLLLAASVFHLLGYGRAIADLLLNFGPAVTGNYIGTLVHENAPRLLA
                     LLPMVVTAVLSSTAAIYLRRARERA"
     gene            complement(1551..>2060)
                     /locus_tag="VHE37_17245"
     CDS             complement(1551..>2060)
                     /locus_tag="VHE37_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT37335.1"
                     /translation="QVFCGPDPCGTLRQRQNAGDVNATGIEGQLTDAISERLNAWGAI
                     ALTNARYAAGQLEGLRPAQAPRATVTGGASWQALDRLSLGAQLHWESQRYDDDRNTLR
                     LGAAFVLDARATYNISERLAAYLALDNLTDANVATAASARVPGDPALGEILSYAQPRM
                     AWLGLSYAP"
BASE COUNT          328 a          662 c          710 g          360 t
ORIGIN      
        1 gccgagcagt ttcgcctttt cggcgcggat ttgcgccagc cgcgaaatgg tgtcgcgggt
       61 gtcgttggcg tcgcccttct cggcgcgcgt ccacgaatgc tcgaacagcg cttggcggac
      121 ggcgcggttt ttcagcgaaa cgagcagcgg ctgctgcgtg gtgttctgaa gcggaatgac
      181 ccatttgccg gcaaggttgc ggtcctgcgc cgccttcgct gcagcggcga tttcctgatc
      241 gctcaaccca tccagatcgg attttttgtc gatgaccagc gcgccggctt tggtggcggc
      301 gagcaatttc tgctggaagg ccgtggaaag cgttgcgtct tccttgttga tggcgcgcag
      361 cttctccttg tcggcatccg acaaattcgc cccggcatgc tcgaactgca ggtaatagat
      421 tttcagcagc tgcgccgatt ccggatcgag ctgcagctgg tcgcgcttgt cgtagagcgc
      481 cttcacccgc tgaaacagct tcgggttgag gaagatagca tcctggtgcg ccgccagttc
      541 cggcgccacc ttggcctgca ccttatcgag cgccggattg gtgttggcgc tgacaatatt
      601 gaagaaggtg gtggacaccc ggtccagcat ccgcccggac ttctccatcg cgacgatggt
      661 gttctcgaag gtcggcgccg ccggatcgtt ggcgatccgc tcgatctcgg ccaggtgctc
      721 cttcatgccc tgctcgaaag ccggctcgta gtcgctgtcc ttgatcttat cgaattccgg
      781 cgcctgcaaa ggcagggtgc tcggctgcgc aaacggattg gtgcttgcgg cggccgtggg
      841 agcgggcgca ggcgcggggg cagcgccggc ggagagccca agcgcggtca cggcggtggc
      901 gcccagcagg aaattgcgga tcgggtgcat gcggtctcct gtcgagggat ggacggcgcc
      961 gccgcccgga ggggttctgc gcaaggttcc aagcatgaaa ccgccgcccg cttcaagccg
     1021 ggtccgggcc gatcacgcga gggtgagcgg tccggtttca cgctcgctcc cgcgcgcggc
     1081 ggagatagat ggcggcggtg ctcgacagca cggccgtgac caccatcggg agcagcgcca
     1141 ggagccgcgg cgcgttttca tgcaccagcg tgccgatgta gttgccggta accgccgggc
     1201 cgaagttcag cagcaggtcg gcgatggcgc ggccgtagcc cagcaagtgg aaaacgctgg
     1261 ccgccagcag cagcgacaac agggcggctg aaatggccgc cgcaaccaga aatcctgcgg
     1321 ccgcaatccg ccgcgtgccg gtaaagaacg acaggcccag caacaccagc gccgccggcg
     1381 cgatcgccca gtcgaaaatg accggcagcc actgaaacgc cgcgaacaga agcttgagca
     1441 tcgggttcta tatgcggcgc attcgcgccc gctcaagccg cggggtgggc ggaacgccgc
     1501 cgcggcttgg tggcgagcca tgcgcattgg cgggagctgc ccccgcgcaa tcagggcgca
     1561 tagctaagac ccagccaggc catgcgcggc tgggcgtatg acaatatttc tcccagcgcc
     1621 ggatcgcccg gcacgcgcgc actggccgcg gtggcgacgt tcgcgtctgt cagattgtcc
     1681 agcgccagat aggcggcgag ccgctcgctg atgttgtagg tggcgcgggc gtccagcacg
     1741 aaggccgcgc ccagacgcag cgtgttccga tcgtcgtcgt agcgctggct ttcccaatgc
     1801 agctgcgccc ccagcgacag gcggtcgagc gcctgccatg acgccccgcc ggtgacggtg
     1861 gcgcgcgggg cttgcgccgg ccgcaatcct tcgagctggc ctgcggcgta gcgcgcattc
     1921 gtcagcgcga ttgcgcccca cgcattcagc cgctccgaga tggcgtcggt gagctgccct
     1981 tcgatgccgg tggcgttcac atcgccggcg ttctggcgct ggcgcagcgt gccgcaggga
     2041 tccggcccgc agaacacctg
//
LOCUS       DASZZC010000801         7215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_640022,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000801 DASZZC010000000
VERSION     DASZZC010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640022"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..741)
                     /locus_tag="VHE37_17250"
     CDS             complement(85..741)
                     /locus_tag="VHE37_17250"
                     /inference="COORDINATES: protein motif:HMM:NF045345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HVT37336.1"
                     /translation="MPLYSPAEAAHYLRLPASTVRAWSFGQAYRDAKGESHRFESVIT
                     VADRKNRRLSFINLVELLVLAAIRRKHRVPLPEVRNAIAYLRKKFPSTHPLSDHAFQT
                     DGVHLFVEKFGQLVNISMDGQIAMKQMIESYLRGVERDPKGIPIKLYLPSRDVQASEP
                     SKVVIDPSRGFGRPVVDGVGVRTEVVLQRFHAGEGISSLAADYALPADVIEDVIRLAA
                     "
     gene            complement(1020..2222)
                     /gene="sat"
                     /locus_tag="VHE37_17255"
     CDS             complement(1020..2222)
                     /gene="sat"
                     /locus_tag="VHE37_17255"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HVT37337.1"
                     /translation="MIQPHGSAELKPLFVSDKSRRDALKAEAAKLPSLLLNSAAAANA
                     VMLGAGYFNPLTGYMNLADAMSVAKDMKTTSGLFWPVPVVNLTKETGVKAGSRIALKD
                     PNVDGQPVIAVMNVKAVENVSDADLKLMAEKIFGTLDPKHPGVATFLGLGNTLLSGDI
                     EVLNYSYFETEFPDTFRTAVQIREEIAKRGWKKVVAFQTRNPMHRAHEELCHMALDRL
                     KADGLVIHMLLGKLKAGDIPAHVRDAAIRKMVELYFKPNSAMVYGYGFDMLYAGPREA
                     LLHAVFRQNMGASYLIVGRDHAGVGSYYGPFDAQEIFKQVPPGALKIEIFGADNTAWS
                     KKLGRVVMMREAPGHTPEDFINLSGTKVREMLGRGEAPPPEFSRPEVAKILSEYYMAE
                     DAAAAKKS"
     gene            complement(2272..3072)
                     /locus_tag="VHE37_17260"
     CDS             complement(2272..3072)
                     /locus_tag="VHE37_17260"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT37338.1"
                     /translation="MQQLLIYLATGALAGVLAGLFGVGGGVIMVPALALVLPTQGVPA
                     AVVMQVALGTSLAVIAITSISSTLAHQRRGGVRWDILLRFAPGLAIGAIAGAYVADHL
                     NAGELKRTFGIGAVLISAQMFSGWQPRGLDEAAAPRTPELLGVGGLIGLLSSLIGIGG
                     GSLTVPYFSARGLAMREAVGTAAASGMPIAWAGAIGFMLAGAGHGTAALPDFSVGYVN
                     LAAFFGLAVASTATAPVGARLAHTLPPQQVKRAFAILLLSTGLKMLLA"
     gene            3108..5039
                     /locus_tag="VHE37_17265"
     CDS             3108..5039
                     /locus_tag="VHE37_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HVT37339.1"
                     /translation="MNPARTLFALAAVLISAAALSAPDDADLAVRQAFRAALLAAEQD
                     RPVSNLQAFARYPLYAYLQAAPLRRSLLKTGHERDADIAAFLDAHDDEPVSRPLRREW
                     YASLGQRQQWKTLLALIPAGTGDAEWRCLRYAALTASAPDTPKLRDDLLQLWLTGETQ
                     PQACIAPFDWLAQQSALTPELKLQRARLALNAGNVDLADELIRALPDEQAAPLRRESR
                     LLREPVKEIPALAARGDPVIAVDSVYAAWDRYIRAKPDDAVALLDPLLKSQDITPPKA
                     GEFVRELALGLAWSRDARALDLFQRMPESARDEHSDEWRVRAALWNGQWALAAGWLAQ
                     MSAEMAAQPRWRYWRARLAEHEHSPQAPQLYRDVLDANNYYAAAAAVRLQQPALPRSQ
                     PAAPRDPVAQKRLNALPALVRARELLRIERDDWARAEWSYALADASDATRVQAAYLAA
                     GWGWYVQAVGALAQARAYDDYTLTHPHPYDVAVLQAAEQSGLRPDWIYGVLRQESLYD
                     PRAVSKRDARGLLQLLLPTARAVARRWNQRAPSADDLYAPDINLTLGSDELREQVERF
                     NGSLPLALCAYNAGSNRPLSWLPPAPMDADIWIENIPFNETRTYVQKIIWNVVVFGWE
                     RTGKGQDVGELFRPIGAVP"
     gene            5036..6262
                     /locus_tag="VHE37_17270"
     CDS             5036..6262
                     /locus_tag="VHE37_17270"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HVT37340.1"
                     /translation="MKRYLVGGAVRDALLGLPVKERDWVVVGATPEDMVAAGYLPVGK
                     DFPVFLHPQSKEEHALARTERKTARGYHGFSFYTGPEVKLEDDLVRRDLTINAIAQDD
                     DGTIIDPHHGRDDLQKKILRHVSAAFAEDPVRILRLARFAARFAPLGFTIAPETIALM
                     KQMVAAGEADALVAERVWQETQRALMGEQPSTFFKVLRECGALARVMPELDALFGVPQ
                     PEKHHPEVDSGVHVLMVVDCAAQRQAPLAVRYACLCHDFGKALSPAHQLPAHHGHEHS
                     GLPLVEKFSERLRVPSACRELALAVTRDHLNVHQAQQLRPATLLTLLERLDAFRKPAR
                     FEEFLLACECDARGRLGLAQRDYPQPQYLRDALAAANAVQVKDVMADGFAGAQIGEEL
                     HRRRAQALAQWKAGRA"
     gene            6299..6832
                     /gene="yjgA"
                     /locus_tag="VHE37_17275"
     CDS             6299..6832
                     /gene="yjgA"
                     /locus_tag="VHE37_17275"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HVT37341.1"
                     /translation="MHHPRPIPNPEAAARYDGPSKTQLKKVMQELQDLGIALLDLPQS
                     AFDELDLDARLRDALEELRKLKDRSARKRQAQFVGKLMRDTDTEPLRRALDANRATHA
                     REVQTLKQIELWRARLLGGDAALDAFAKAHPSADVSALRVLVREAQRERTTEAGNGKA
                     YRELFAHLRRVLQAAQA"
     gene            complement(6835..>7215)
                     /locus_tag="VHE37_17280"
     CDS             complement(6835..>7215)
                     /locus_tag="VHE37_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVT37342.1"
                     /translation="GWAGALIAGLAQIVAAVFPGTSRSAAAIFALMLAGTGERAAATE
                     FVFLLGIPTMFAASAAELHGALKQGAPAENWTDLGIAFVVSMIVAFIAVKWLLRYIQT
                     HRFTAFAIYRIALGAALLVFARSG"
BASE COUNT         1224 a         2395 c         2465 g         1131 t
ORIGIN      
        1 gccggccagg cgcagcgcct ctacaacgta tttgctgccg agactgcgat caaccaggaa
       61 cgtgatccgc ggcaaggcgc aggactacgc ggccaggcgg atgacatctt cgatgacatc
      121 tgccggcaat gcgtagtcgg cggccagcga tgagatccct tcgcctgcat gaaagcgctg
      181 caagacgact tcagtgcgca cgccgacgcc gtcgacgaca gggcggccga atccccggct
      241 gggatcgatg accactttgc tgggctcact cgcttgcaca tcgcgggaag gcaggtacag
      301 cttgatcggt attcccttgg ggtctctttc cacgccgcgc agataggatt cgatcatctg
      361 cttcatggcg atctgaccgt ccatggaaat gttgaccagt tgcccaaatt tttccacgaa
      421 caggtgcacg ccatcggtct ggaaagcgtg atcggacaag ggatgcgtgc tggggaattt
      481 cttgcgcaga tatgcgatgg cgttgcgcac ttctggaagc ggaacgcggt gcttgcgccg
      541 gatggcggcg agcaccagca gctcgaccag attgatgaat gacaaccggc gatttttgcg
      601 atcggccacg gtgatgaccg attcaaaccg gtgagactcg cccttggcat cgcgataggc
      661 ctgtccgaaa gaccacgcgc gcacggtcga ggctggcaag cgcaggtagt gcgctgcttc
      721 tgccggcgag tacagcggca tttcgtagag gtctttggcg gaagtgcgag acatggtggc
      781 cctcatgcgc gggaaagatt ataggcctta ctcatcggcg accgataagc atccagcagt
      841 agcaaacagc acaaaaacct cctaccgcat ctgcattttc aatgttatta tttattatgt
      901 atttttataa taatactata tttaataaca gtcaaaacat gcaggcggga agggcatttg
      961 ctgaccttcg gacataaaaa acgcgggccg aagcccgcgt ctttggcgac gctcgttact
     1021 caggatttct tcgcggccgc cgcgtcctcg gccatgtagt actcggacag gatcttcgcc
     1081 acttccgggc gcgagaattc cggcggcggc gcttcgccgc ggccgagcat ctcgcgcacc
     1141 ttggtgcccg acaggttgat gaagtcctcc ggcgtgtggc ccggcgcttc gcgcatcatc
     1201 accacgcggc ccagcttctt gctccacgcg gtgttgtcgg cgccgaagat ctcgatcttg
     1261 agcgcgccgg gcggcacctg cttgaagatt tcctgcgcgt cgaacgggcc gtagtagctg
     1321 cccacgccgg cgtggtcgcg gccgacgatc aggtagctcg cgcccatgtt ctggcggaac
     1381 accgcgtgca gcagcgcctc gcgcgggccg gcgtacagca tgtcgaaacc gtagccgtag
     1441 accatcgccg agttcggctt gaagtacagc tccaccatct tgcggatggc ggcgtcgcgc
     1501 acgtgcgcgg gaatgtcgcc ggccttgagc ttgcccagca gcatgtggat caccaggcca
     1561 tcggccttga ggcgatcgag cgccatgtgg cacaactctt cgtgcgcgcg gtgcatcggg
     1621 ttgcgcgtct ggaatgccac cactttcttc cagccgcgct tggcgatctc ctcgcggatc
     1681 tgcacggcgg tgcggaaggt gtcggggaat tcggtctcga agtagctgta gttcagcact
     1741 tcgatgtcgc ccgacagcag cgtgttgccc aggccgagga acgtcgccac gccgggatgc
     1801 ttgggatcga gcgtgccgaa gattttttcc gccatcagct tgaggtcggc gtcgctcacg
     1861 ttctccaccg ccttcacgtt catcacggcg atgaccggct ggccgtcgac gttcggatcc
     1921 ttcagcgcga tgcgcgagcc ggccttcacg ccggtttcct tggtcaggtt gaccaccggc
     1981 accggccaga acaggcccga ggtggtcttc atgtccttgg ccaccgacat cgcatcggcc
     2041 aggttcatgt agccggtcag cgggttgaag tagccggcgc ccagcatcac cgcgttggcg
     2101 gcggcggcgg aattgagcag cagcgagggc agcttggcgg cctccgcctt cagcgcgtcg
     2161 cggcgtgact tgtcggaaac gaacagcggc ttgagttcgg cggagccgtg gggctggatc
     2221 atggtcgggg cgtgcgtctg cggtgcgagt tggggcgcgg attataaaag actatgcgag
     2281 cagcatcttg agcccggtgg acagcagcag gatggcaaat gcccgcttga cctgctgcgg
     2341 cggcagcgtg tgcgccagcc gggcgccgac cggcgcggtc gcggtgctgg ccacggccag
     2401 gccgaagaag gcggcgaggt tgacgtagcc cacggaaaaa tccggcagcg ccgcggtgcc
     2461 gtggccggcg cctgccagca tgaagccgat cgcgccggcc caggcgatcg gcatgccgga
     2521 ggccgcagcg gtgcccacgg cctcgcgcat ggccagtccg cgcgcggaaa aatatggaac
     2581 cgtgagcgag cccccgccga tgccgatcag cgacgacagc aggccgatca gcccgccgac
     2641 gcccagcagt tccggcgtgc gcggagccgc cgcttcgtcc aggccgcgcg gttgccagcc
     2701 ggaaaacatc tgcgccgaga tcagcaccgc gccgatgccg aaagtgcgct tcagttcgcc
     2761 ggcgttcagg tgatcggcca cgtaggcacc ggcgatggcg ccgatggcca ggcccggcgc
     2821 gaagcgcagg aggatgtccc agcgcacgcc gccgcggcgc tggtgtgcga gcgtcgagga
     2881 gatcgaggtg atggcgatca ccgccagcga agtgcccagc gccacctgca tcaccaccgc
     2941 cgccggcacg ccctgcgtgg gcagcaccag cgccagcgcc ggcaccatga tcacgccgcc
     3001 gcccacgccg aacagtccgg ccagcacgcc cgccagtgcg ccggtggcaa gatagatcag
     3061 caactgctgc acgtgcggcc tgtcctaaaa tgagtcgcgc atcctacatg aaccccgcac
     3121 gcacactctt cgcattggcc gccgtgctga tctcggccgc agcgttgtcc gccccggacg
     3181 acgccgacct cgccgtgcgc caggcattcc gcgccgcgct gctggccgcc gagcaggacc
     3241 ggccggtgag caacctgcag gctttcgcgc gctacccgct gtacgcctac ctgcaggcgg
     3301 cgccgctgcg gcgctccttg ctgaagacgg gacatgagcg cgatgccgat atcgccgcct
     3361 tcctcgatgc gcacgacgac gagccggtgt cgcgcccgtt gcgccgcgag tggtatgcga
     3421 gtctcggtca gcgccagcaa tggaagacgc tgctcgcgct gatacccgcc ggcaccggcg
     3481 atgccgagtg gcgctgcctg cgctatgccg cgctcactgc gagcgcgccg gacacgccga
     3541 agctgcgcga cgacctgctg cagctgtggc tcaccggcga gacgcagccg caggcctgca
     3601 tcgccccgtt cgactggctg gcgcagcaat ctgcgctgac gccggagctg aaactgcagc
     3661 gcgcgcggct ggcgctcaac gcgggcaatg tcgatctggc cgatgagctg atccgcgcgc
     3721 tgcccgacga gcaggccgcg ccgctcaggc gcgagtcgcg cctgctgcgc gagccggtga
     3781 aggagatccc cgcgttggcg gcgcgcggcg acccggtgat cgccgtcgac agcgtgtacg
     3841 ccgcgtggga tcgctacatc cgcgcaaaac ccgatgacgc agtggcgctg ctcgatccgc
     3901 tgctgaagag ccaggacatc acgccaccca aggccggcga gttcgtgcgc gagctcgcgc
     3961 tgggcctggc ctggagccgc gatgcgcgcg cgctcgacct gttccagcgc atgcccgaat
     4021 ccgcgcgcga cgagcacagc gatgaatggc gcgtgcgcgc cgcgctgtgg aacgggcagt
     4081 gggcgctggc cgccggctgg ctggcgcaga tgagcgcgga gatggcagcg cagccgcgct
     4141 ggcgctactg gcgcgcgcgc ctggccgagc atgagcactc gccgcaggcg ccgcagctat
     4201 accgggatgt gctcgatgcc aacaactact acgcggcggc agctgctgtg cgcctgcagc
     4261 agcccgcgct gcccagatcg cagccggcgg cgccgcgcga cccggtggcg cagaagcgcc
     4321 tgaacgcgct gccggcgctg gtgcgcgcgc gcgaactgct gcgcatcgag cgcgacgact
     4381 gggcgcgcgc cgagtggagc tatgcgttgg ccgatgccag cgacgccacg cgcgtgcagg
     4441 cggcctatct cgccgcgggc tggggctggt acgtgcaggc ggtcggcgcg ctggcgcagg
     4501 ccagggccta cgacgattac acgctcaccc atccgcatcc ctacgacgtg gcggtgttgc
     4561 aggccgccga gcagagcggg ctgcgcccgg actggatcta cggcgtgctg cgccaggaga
     4621 gcctgtacga tccgcgcgcg gtgtccaagc gcgatgcgcg cgggctgctg cagcttctgc
     4681 tgcccaccgc gcgcgcggtg gcgcggcgct ggaaccagcg cgcgccgtcg gccgacgacc
     4741 tttacgcgcc ggacatcaat ctcacgctgg gctcagacga gctgcgcgaa caggtcgagc
     4801 gcttcaacgg cagcctgccg ctggctctgt gcgcgtacaa cgccggcagc aaccggccgc
     4861 tgtcatggct gccgcccgcg ccgatggacg ccgacatctg gatcgagaac attccgttca
     4921 acgaaacgcg cacctacgtg cagaagatca tctggaacgt ggtggtgttc ggctgggagc
     4981 gcacgggcaa ggggcaggac gtgggcgagt tgttcaggcc gatcggagcg gtgccatgaa
     5041 gcgctacctg gtcggcggcg cggtgcgcga cgccctgctc ggcctgccgg tgaaggagcg
     5101 cgactgggtg gtggtcggcg ccacgccgga agacatggtc gcggccggct acctgccggt
     5161 aggcaaggat tttccggtgt tcctgcatcc gcagagcaag gaagaacacg cgctggcgcg
     5221 caccgagcgc aagaccgcgc gtggctatca cggcttctcc ttctacaccg gccccgaggt
     5281 gaagctcgaa gacgacctgg tgcggcgcga cctcaccatc aacgcgattg cgcaggacga
     5341 cgatggaacg atcatcgatc cgcatcatgg ccgcgacgac ctgcagaaga aaatcctgcg
     5401 ccatgtttcc gcggcctttg ccgaagatcc cgtgcgcatc ctgcggctgg cgcgcttcgc
     5461 cgcgcgcttt gcgccgctgg gtttcaccat cgcgcccgaa accatcgcct tgatgaagca
     5521 gatggtcgcc gccggcgaag ccgatgcgct ggtggccgag cgcgtgtggc aggaaacgca
     5581 gcgcgcgctg atgggcgagc agcccagcac gttcttcaag gtgctgcgcg agtgcggcgc
     5641 gctggcgcgc gtcatgccgg aactggacgc gctgttcggc gtgccgcaac cggagaagca
     5701 tcacccggaa gtggacagcg gcgtgcacgt gctgatggtg gtggactgcg ccgcgcagcg
     5761 ccaggcgccg ctcgcggtgc gctacgcctg cctgtgccac gacttcggca aggcgctgtc
     5821 gccggcgcac cagctgccgg cccatcacgg ccacgagcac agcgggctgc cgctggtgga
     5881 gaaattttcc gagcggctgc gcgtgccatc ggcctgccgc gaactggcgc tggcggtgac
     5941 gcgcgaccat ctcaacgtgc accaggcgca gcagctgcgc ccggccacgc tgctcacgct
     6001 gctggagcgg ctcgatgcct tccgcaagcc ggcgcgcttc gaggagttcc tgctcgcctg
     6061 cgaatgcgat gcgcgcggcc gcctgggtct tgcgcagcgc gactatccgc agccgcaata
     6121 cctgcgcgat gcgctggccg cggccaacgc ggtgcaggtg aaggacgtga tggcggacgg
     6181 ttttgccggc gcgcagatcg gcgaagaact gcatcgccgc cgcgcgcagg cgttggcgca
     6241 gtggaaggcc ggcagggcat aacatgagca agctggcctg atgccgccct gacccgacat
     6301 gcaccatcca cgtcccattc ccaatcccga agcggccgcc cgctatgacg gcccgagcaa
     6361 gacccagctg aagaaggtca tgcaggaact gcaggatctc ggcattgccc tgctcgatct
     6421 gccgcagtct gccttcgacg agctggacct tgatgcgcgc ctgcgcgatg cgctggagga
     6481 gctgcgcaag ctgaaggacc gcagcgcgcg caagcgacag gcccagttcg tcggcaagct
     6541 gatgcgcgac accgacaccg agccgctgcg ccgcgcgctg gatgccaacc gcgcaacgca
     6601 cgcgcgcgag gtgcagacgc tcaagcagat cgagttgtgg cgcgcacgcc tgcttggcgg
     6661 cgatgccgcg ctggatgcct tcgccaaggc gcatccgtca gccgatgttt ccgccctgcg
     6721 cgtgctggtg cgcgaagcgc aacgcgaacg cactactgag gcgggcaacg gcaaggccta
     6781 ccgcgagtta ttcgcgcacc tgcggcgggt gctgcaggct gcgcaggcct gacttcagcc
     6841 agaccgcgcg aagacgagca gcgcggcgcc cagcgcaatg cggtagatag cgaaggcggt
     6901 gaagcggtgg gtctggatgt agcgcagcag ccacttcacc gcgatgaacg ccacgatcat
     6961 cgacaccacg aaggcgatgc ccaggtcggt ccagttctcc gccggtgccc cttgcttcag
     7021 cgcgccgtgc agttccgcgg cgctggcggc gaacatggtg ggaatgccga gcaggaagac
     7081 gaattccgtc gccgccgcgc gctcgccggt gccagcgagc atcagcgcga agatggcggc
     7141 ggccgagcgc gaggtgccgg gaaacaccgc agcgacgatc tgcgccaggc cggcgatcaa
     7201 cgcgccggcc cagcc
//
LOCUS       DASZZC010000802         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_706323,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000802 DASZZC010000000
VERSION     DASZZC010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706323"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHE37_17285"
     CDS             complement(<1..363)
                     /locus_tag="VHE37_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT37343.1"
                     /translation="MNQPLYHNALRGILSLEAYTPGMPIDELQRRLGVKDVIKLASNE
                     NPLGPSPRVKAALAAAVSGENLALYPDGSGFRLKQKLGKFHDIDPSRITLGNGSNDIL
                     EFLARIYAGPGRAVMFSAH"
     gene            complement(385..1467)
                     /gene="pheA"
                     /locus_tag="VHE37_17290"
     CDS             complement(385..1467)
                     /gene="pheA"
                     /locus_tag="VHE37_17290"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HVT37344.1"
                     /translation="MSDELQQARTQIDALDQQIQQLISQRARIAQQVAHIKQRMGAVG
                     DHYRPSREAEVLRSAIARNQGPLTDEVMARLMREIMSACLSLESPLTVAYLGPEGTYT
                     PPAVQKQFGDSVSERPAAAIDEIFRDVESGAADYGVVPVENSTGGIVIHTLDALVNTK
                     LRICGEVALPVHHHLLSRETSMNQVKHIYSHAQSFAQCRKWLDARLPNAERETVSSNG
                     QAAKLAREKGGAAIASLQAGRINQLNILASNIEDEPDNTTRFLVIGKQDPAPTGEDIT
                     SIVLSAHRNQPGALFGLLQPFADARLDMTRIESRPAKGGVVQDYYFFIDFRGHAHEPN
                     VRKALDLVRAGAAFFKILGSYPRAVL"
     gene            complement(1464..2639)
                     /locus_tag="VHE37_17295"
     CDS             complement(1464..2639)
                     /locus_tag="VHE37_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HVT37345.1"
                     /translation="MFKIQTLNNISVYGLERLPRDRYEIASEIQNPDAILVRSAEMHK
                     LEIPASIKAIGRAGAGTNNIPVAKMSQRGVPVFNAPGANSNAVKELVLASLFMAARNI
                     PQSLDFVKKLDGDDKAMHEAVEAGKKKFVGFELPGRTLGVIGLGAIGVKVANAALELG
                     MTVYGYDPAMTVQNAWQLNSGVKQALSVDDLVTKSQFISVHVPLLDATRGLINADRLK
                     MMKKQGVILNFARAEIVDEKAVVDALNAGTLHAYACDFPSVALKGHPRVIATPHLGAS
                     TGEAEDNCAIMVADQIRDFLEVGNVRNSVNFPEAVMPLSAGTTRVCIVNANVPNMVGQ
                     ITTALAKRNLNIADLLNKSRGDIAYTLVDVDSQVPAELEKEIRAIAGVLAVRVIEAK"
     gene            complement(2658..3740)
                     /gene="serC"
                     /locus_tag="VHE37_17300"
     CDS             complement(2658..3740)
                     /gene="serC"
                     /locus_tag="VHE37_17300"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HVT37346.1"
                     /translation="MKRIHNFSAGPATLPEAVLKQVQAELLDWNGTGMSVMEMSHRDK
                     PFMSIAQAAEADLREVMGVPANYKVLFLQGGATAQFTYLPMNLLRGKTGADYVLTGDW
                     GKKAAKEAGRYCKVNVAASSEADKFTHVPDRAGWKLDPNAAYVHITSNETIHGVEFAA
                     TPDVGSVPLVSDMSSTIMSRPVDVTKYGLIYAGAQKNIGPAGLTIVIVRDDLIGQAAA
                     NTPGIFDYKQMADNESMLNTPPCFAWYVSGLVFKHIKAQGGLAAMAKLNERKAGLLYD
                     YIDSEPFYTNPVKKADRSWMNVPFTLKDAKLDEEFLKGAKAASLSGLKGHRSVGGMRA
                     SLYNALPEQAVTALVDYMKEFVRTRG"
BASE COUNT          634 a         1216 c         1186 g          784 t
ORIGIN      
        1 atgcgccgaa aacatcacgg cgcggccggg ccccgcgtag atgcgggcca ggaattcgag
       61 gatgtcattc gatccgttgc ccagtgtgat ccgcgacgga tcgatgtcgt gaaattttcc
      121 aagcttttgc ttgagccgga atccgctgcc gtccgggtag agcgccaggt tctcgccgct
      181 gactgcagcg gcgagggccg ctttgaccct ggggctgggg ccgagcgggt tctcgttcga
      241 cgcgagcttg atcacgtcct tcacgcccag ccggcgctgc agttcgtcga tcggcatgcc
      301 gggtgtgtac gcctcgagcg acaggattcc gcgtagggcg ttgtggtaca gcggctggtt
      361 catcagatgc accgaaaagc gcggctagag cacggcgcgt ggataagagc cgagtatttt
      421 gaagaaagcg gcgccggccc gcacaagatc aagcgctttg cgcacattcg gctcgtgggc
      481 gtgtccgcga aaatcgatga agaaatagta gtcctgcact acgccccctt ttgcggggcg
      541 cgattcgatg cgggtcatgt cgagcctggc atcggcgaaa ggctgcagca gcccgaacag
      601 cgcacccggc tggttgcgat gcgcgctcaa cacgatggag gtgatgtctt cgccagtcgg
      661 cgccgggtcc tgtttgccga tgaccaggaa ccgcgtggtg ttgtccggct catcttcaat
      721 gttggacgcc aggatattga gctggttgat gcggcctgcc tgcaggctgg cgatcgccgc
      781 cccgcccttc tcacgcgcca gcttggccgc ctggccattg ctcgatacgg tctcgcgctc
      841 ggcgttgggc aggcgtgcgt ccagccactt gcggcattgc gcgaacgact gcgcatgcga
      901 gtagatgtgc ttgacctggt tcatgctggt ttcgcgcgac agcaggtgat ggtgcaccgg
      961 cagcgccacc tcgccgcaga tacgcagctt ggtgttgacc agcgcatcca gcgtgtggat
     1021 gacgatgccg ccggtcgaat tctccaccgg caccacaccg taatcggccg cccccgattc
     1081 cacgtcgcgg aagatctcgt cgatggcagc agccgggcgc tcgctcacgc tgtcaccgaa
     1141 ctgcttctgc actgccggcg gggtataggt gccttcgggc ccaagatagg ccaccgtcaa
     1201 cggtgattcc aatgacaggc aggcagacat gatctcgcgc atcaggcgcg ccatgacctc
     1261 gtcggtcagc ggcccctggt tgcgggcgat ggcgctgcgc agcacctcgg cctcgcgcga
     1321 cgggcgatag tggtcgccca ccgcgcccat cctctgcttg atgtgcgcca cctgctgggc
     1381 aatgcgcgcg cgctggctga tcagctgctg gatctgctga tccagcgcat cgatctgcgt
     1441 gcgcgcctgc tgcagctcgt cgctcatttg gcttcgatca cccgcaccgc aagcaccccg
     1501 gcgatcgcgc ggatctcctt ttccagttcc gccggcacct ggctgtcgac atcaaccagc
     1561 gtgtatgcaa tgtcaccgcg cgacttgttc agcaggtcgg cgatgttgag attgcgcttg
     1621 gccagggccg tggtgatctg gccgaccatg ttcggcacgt tggcgttgac gatgcatacg
     1681 cgcgtggtgc ctgccgaaag cggcatcact gcttcgggga aattgaccga gttgcgcacg
     1741 ttgcccactt cgaggaagtc gcggatctgg tcggcgacca tgatcgcgca gttgtcctcc
     1801 gcttcgccgg ttgacgcacc caggtgcgga gttgcaatca cccgtggatg tcccttgagc
     1861 gcaacgctgg gaaaatcgca ggcataagcg tggagcgtgc cggcattgag cgcatcgacc
     1921 acggctttct cgtcgacgat ctcggcacgg gcgaaattca ggatcacgcc ctgcttcttc
     1981 atcatcttca ggcgatcggc gttgatcaac ccacgtgtgg cgtcgagcag cggcacgtgc
     2041 accgagatga actgcgactt cgtcaccagg tcatcgaccg acagggcctg cttgacgccg
     2101 gagttgagct gccaggcatt ttgcaccgtc attgcggggt cgtagccgta gacggtcatg
     2161 cctagttcca gcgcggcatt tgccaccttg acgccgatgg ctcccagccc gatcacgccc
     2221 agcgtgcggc ccggcagttc aaagccaacg aacttcttct tgccagcctc caccgcctcg
     2281 tgcatggcct tgtcgtcgcc gtcgagcttc ttcacgaagt ccagcgactg cggaatgttg
     2341 cgcgctgcca tgaacagcga ggcgagcacc agttccttca ccgcgttgga attggcgccg
     2401 ggagcgttga acaccggcac gccgcgctgc gacatcttgg caacagggat gttgttggtc
     2461 ccggcaccgg cacggccgat ggccttgatc gaggccggga tctccagctt gtgcatctcc
     2521 gccgagcgca ccaggatcgc gtccgggttc tggatctcgg aagcgatttc gtaacggtcg
     2581 cgcggcaggc gctcgaggcc gtaaacggag atgttgttga gcgtttgaat cttgaacatg
     2641 ggtatcggtt tcctaagtca gccgcgagta cgcacgaact ctttcatgta gtcgaccagc
     2701 gccgtcactg cctgctcggg gagcgcattg tagaggctcg cgcgcatgcc tcccaccgag
     2761 cggtgaccct tgaggcccga caggctggct gctttcgcac ccttgagaaa ttcctcatcg
     2821 agcttggcgt cctttagcgt gaacggcacg ttcatccacg aacggtccgc cttctttacc
     2881 gggttggtat agaacggctc gctgtcgatg tagtcgtaca gcagcccggc cttgcgctcg
     2941 ttgagtttgg ccatggccgc gagcccgccc tgcgccttga tgtgcttgaa caccaggccc
     3001 gagacgtacc aggcaaagca tggcggcgtg ttcagcatcg actcgttgtc cgccatctgc
     3061 ttgtagtcga agatgcccgg cgtgttcgcg gcagcctggc cgatcaggtc gtcgcgcacg
     3121 atgacaatgg tcagccccgc aggcccgatg ttcttctgcg cacccgcata gatcaggccg
     3181 tatttggtca catcgaccgg cctagacatg atggtcgacg acatgtcgga caccagcggc
     3241 acgctgccga cgtccggcgt cgctgcgaat tccacaccgt ggatcgtctc gttcgaggtg
     3301 atgtgcacgt acgcggcatt cgggtccagt ttccagccgg cacgatcggg cacatgcgtg
     3361 aacttgtccg cttcgctgct ggccgcgaca ttcaccttgc aatagcggcc cgcttccttg
     3421 gccgccttct tgccccagtc gccggtcagc acgtagtccg cgccggtttt gccgcgcagc
     3481 aggttcatcg gcaggtaggt gaactgcgcg gtcgcgccac cctgcaggaa cagcaccttg
     3541 tagttggccg gcacgcccat cacttcgcgc aggtccgcct cggccgcctg ggcgatcgac
     3601 atgaacggct tgtcgcggtg ggacatttcc atcaccgaca tgccggtgcc gttccagtcg
     3661 agcagttccg cctgcacctg cttgagcacc gcttccggca acgtcgccgg gcccgcgctg
     3721 aaattatgaa tacgcttcat cggaggttcc tgtttgcttg tctcaaggtt taaccgctac
     3781 acccagccag ttctcgactt ccagtcccgg caccgctgca
//
LOCUS       DASZZC010000803         4472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_706684,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000803 DASZZC010000000
VERSION     DASZZC010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706684"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..459)
                     /locus_tag="VHE37_17305"
     CDS             complement(193..459)
                     /locus_tag="VHE37_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37347.1"
                     /translation="MALQISKATSVGVDAQYWRIGLLNFHPRTASCQVVMDGYPTHEI
                     RETGAQPIDQRALDLPFEQCGDGDRAAVYDVIKQLPDWTGATDV"
     gene            complement(462..782)
                     /locus_tag="VHE37_17310"
     CDS             complement(462..782)
                     /locus_tag="VHE37_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37348.1"
                     /translation="MKINADAVVTNLDGEPLHEGKTAKGEPRQLTVGRAIVNALLADY
                     PNEQATGDEKLRRYTLAKKFHAGGEVDVKAESAALIKQLVPRAWAPMVVGPVFEAIDG
                     QAGE"
     gene            complement(801..1517)
                     /locus_tag="VHE37_17315"
     CDS             complement(801..1517)
                     /locus_tag="VHE37_17315"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HVT37349.1"
                     /translation="MFAAAAALLCANKPDPYLSSVVALLHMNGANNGTTFTDQIGHTF
                     TANGDAKTATDISKFNGSDGKFDGSGDYLTTPHASAFDLHSGDWTIECWIYLQATTAG
                     NQDLIQKDGVFGSSFSQYELSVNSSGKLRGSLGTGNGTSSSQTFTGTTTITQNAWHHV
                     AMVRFGSLCLGFLDGGLEWSTTITATITDGGKALYIGYQQGQPSSSYFNGRMAEVRIT
                     KGVARYTAAFNPPAVAFPDS"
     gene            complement(1517..3916)
                     /locus_tag="VHE37_17320"
     CDS             complement(1517..3916)
                     /locus_tag="VHE37_17320"
                     /inference="COORDINATES: protein motif:HMM:NF022436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HVT37350.1"
                     /translation="MSDQTPRLDFSLMSASQAQKEVTFNDAMRALDVLVQGRVLDMDL
                     ATPPASPDDGDAYIVAASPTGLWTGRATQIATWFNGVWNFVAPDDGFALWVVSKTKRY
                     TFTGSAWTSAAEFDSISPMTTLGDIIYGGASGAGTRLPGNTSATKKFLSQTGSGSASA
                     APAWAQVTAADIVTTTVASALIALANPSAISFLRINADNSVSARSASNFRTDLGLGTG
                     DSPTLAGLTTTGDVIIGGNLTVNGTTTHVNSTTVDYDDGMLKLADGNASDTLDEGWYC
                     QYQPSATPLYAGLFRDASDGKFKLYKDLQSEPTTTVNIGGTGYTAATLTVGGIEVTGL
                     TSGRVPYATSGGALTDSSALQFTGARLLVGGPTDDGASVLQLQVSAATAITFGSPNPI
                     VVTTVSDSSSVAIGQRRARAGTLAVQTDDILGTNQFSGYDGGAYGVGGFLRFFAAENW
                     ASGTNRGTYADIFTTPTGSTTNTRAMRFGANGAIGIGGLSDPDAFLTISANATLSGPQ
                     IRLRSRRAAMTSGSMIGGMDFMSNDTDLTAPGTVVGLFQCLANQTHTASQLGTKFQWS
                     LTANGATSTSDLMVLTGGGALIVNTPTDDGINKLQVNGGAAIAGTLTLSAQNIATDTS
                     TGMKIGTSTSQKLGFFNATPVTQQSGSTDVLASLVNLGLRAASSNPGFNIGTGAIACG
                     AITAAGVCAVTPYRANISGAVSIDLTGTAKSNELILTLTGNVTSFALTNPTDGATYNI
                     RFIQDGTGGRTVSGLSSKFKFAGGTPPVFSSAAGAVDFMSADYGATEDTYMAGFSKGM
                     A"
     gene            complement(3925..>4472)
                     /locus_tag="VHE37_17325"
     CDS             complement(3925..>4472)
                     /locus_tag="VHE37_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37351.1"
                     /translation="AAYGISAQYGYTGLVSGQVAVVVDDTITPTPVKLDYLASSTSDS
                     DFYPFLTAVRHTVPNLPSGRAQASSVQFAPVQGTVAGDAVGLAFHVRLGASVFSYTPG
                     SGDVSGGALDYDAIYNALKALIDASGSWTAALQDGSIWVALAPTDFYRTVLVITATAA
                     NTPFTVDAWLGSAIVTAELSS"
BASE COUNT          656 a         1349 c         1661 g          806 t
ORIGIN      
        1 cgcagcggta ggtgccggcg ggaatgcagc tcttgccgtt ggcgttgttg cgccagggca
       61 gctccagtga tgccacgctg ccgccgcccg gcaggctgat ggagccgaag gtgccctggt
      121 cgtccgtgac caggcgttga aggatgactt gggtcatggt gttgcctccc cgggtgagcg
      181 aatggaacgc cgttaaacgt cggtggcgcc ggtccagtcc ggcagttgct tgatcacgtc
      241 gtaaaccgcg gcgcggtcgc cgtcgccgca ctgctcgaac ggcagatcga gcgcgcgctg
      301 gtcgatcggc tgcgcgccgg tctcgcggat ctcgtgcgtg ggatagccgt ccatcaccac
      361 ctggcagctc gcggtgcgcg ggtggaagtt gagcaggccg atgcgccagt actgcgcgtc
      421 gacgccgacc gaggtggcct tgctgatttg cagcgccatg gctattcccc cgcctggccg
      481 tcgatggcct cgaacaccgg gccgaccacc atcggcgccc aggcgcgcgg caccagctgc
      541 ttgatcaaag cggcgctctc cgccttcacg tcgacttcgc cgccggcgtg gaatttcttg
      601 gccagcgtgt agcggcgcag cttctcgtcg ccggtggctt gctcgttggg gtagtcagcg
      661 agcagcgcat tgacgatggc gcggccgacg gtgagctggc gcggctcgcc cttggcggtc
      721 tttccttcat gcagcggttc gccgtcgaga ttggtgacca ccgcgtctgc attgattttc
      781 atgggtgaaa ctcctggttg ttatgaatct ggaaacgcaa ctgccggcgg gttgaatgcc
      841 gccgtgtagc gcgccacgcc cttggtgatg cgcacctcag ccatgcggcc gttgaagtag
      901 ctgctggacg gttggccctg ctggtagccg atgtaaagcg ccttgccgcc atcggtgatc
      961 gtggcggtga tggtcgtgga ccattccagg ccgccatcga ggaagcccag gcacaggctg
     1021 ccgaagcgca ccatggcaac gtggtgccag gcgttttgcg tgatggtggt cgtgccggtg
     1081 aaggtctggc tggagctggt gccgttgccg gtacccagcg agccgcgcaa cttgccactg
     1141 ctgttgaccg acagctcgta ctgcgagaac gaggacccga acacgccgtc tttctggatc
     1201 aggtcctggt tgccggcggt ggtggcctgc aggtagatcc agcactcaat cgtccagtcg
     1261 ccgctgtgca ggtcgaaggc gctggcatgc ggcgtggtca gataatcgcc gctgccatcg
     1321 aacttgccgt ccgatccgtt gaattttgaa atgtccgtgg ccgtcttggc atcgccattg
     1381 gcggtgaagg tgtggccgat ctggtcggtg aacgtggtgc cgttgttggc gccgttcatg
     1441 tgcagcagcg ccaccacgct ggacaggtaa gggtccggct tgttggcgca tagcagcgcg
     1501 gcggcggcgg cgaacatcag gccatgccct tggagaagcc ggccatgtag gtgtcttccg
     1561 tggcgccgta atccgcgctc atgaagtcca ccgcaccggc cgccgacgag aacaccggcg
     1621 gcgtgccgcc ggcgaacttg aacttggacg acagcccgct gaccgtgcgg ccgccggtgc
     1681 cgtcctggat gaagcggatg ttgtaggtgg cgccgtcggt ggggttggtc agcgcgaacg
     1741 aggtgacatt gccggtgagc gtgaggatca gctcgttgga cttggccgtg ccggtcaggt
     1801 caatgctgac cgcgccgctg atgttggcgc ggtatggcgt gacggcgcac acgccggcgg
     1861 cggtgatggc gccgcaggcg atggcgccgg tgccgatgtt gaagccgggg ttggacgagg
     1921 ccgcgcgcag gcccaggttg accagcgagg ccagcacgtc cgtgctgccg ctctgctgcg
     1981 tgaccggcgt ggcgttgaag aagcccagct tctggctggt gctggtgccg atcttcatgc
     2041 cggtgctggt gtcggtggcg atgttctgcg cgctcaacgt aagcgtgccg gcgatggccg
     2101 cgccgccgtt gacctgcagc ttgttgatgc cgtcatccgt gggggtattg acgatcagcg
     2161 caccgccgcc ggtgagcacc atcagatcgc tggtgctggt ggcgccgttg gcggtgaggc
     2221 tccactggaa cttggtgccc agctgcgatg cggtgtgcgt ctggttggcc aggcactgga
     2281 acaaaccgac cacggtgccc ggcgcggtca gatccgtgtc gttggacatg aagtccatgc
     2341 cgccgatcat cgagccgctg gtcatggcgg cgcggcgcga gcgcaggcgg atctgcgggc
     2401 cggatagggt ggcgttagcg gagatggtca ggaaggcatc gggatcgctg aggccgccga
     2461 tgccgatggc accattggcg ccgaagcgca tggcgcgcgt attggtggtc gagcccgtgg
     2521 gcgtggtgaa aatgtccgca taggtgccgc ggttggtgcc ggaggcccag ttctccgcag
     2581 cgaagaagcg caggaatccg ccgacgccgt aagcgccgcc gtcatagccg gagaactggt
     2641 tcgtgccgag gatgtcgtcg gtctgcacgg ccagcgtgcc tgcgcgcgcg cggcgctggc
     2701 cgatggcgac cgagctggag tcgctgacgg tggtgacgac gatgggatta gggctgccga
     2761 aggtgatggc ggtagctgcg ctgacctgca gctgaaggac cgatgcgcca tcatcggtag
     2821 gcccgccgac cagcaggcgc gcaccggtga attgcagcgc cgagctgtcc gtcagcgcgc
     2881 cgccgctggt ggcatagggc acgcggccgg aggtgaggcc ggtgacctcg atgccgccca
     2941 cggtgagcgt ggccgcggtg tagccggtgc cgccgatgtt gacggtggtg gtcggctccg
     3001 actgcagatc cttgtacagc ttgaacttgc cgtcgctggc gtcgcggaac aggcccgcgt
     3061 acagcggcgt ggcggacggc tggtactggc agtaccagcc ttcatcgagc gtgtcgctgg
     3121 cgttgccgtc tgccagcttg agcatgccgt cgtcgtagtc gacggtggtg ctgttgacgt
     3181 gcgtggtggt gccattgacg gtcaggttgc cgccgatgat cacgtcgccg gtggtggtga
     3241 gcccggccag cgtgggcgag tcgccggtgc ccaggcccag gtcggtgcgg aagttggacg
     3301 cgctgcgcgc gctcaccgag ttgtcggcgt tgatgcgcag gaagctgatg gcgctggggt
     3361 tggccagcgc gatcagcgcc gatgccacgg tggtggtgac aatgtcggcg gcggtcacct
     3421 gcgcccatgc cggcgcagcc gaggcgctgc cgctgccggt ctggctcaga aatttcttgg
     3481 tggcgctggt gttgccgggc agccgcgtgc cggcgcccga ggcgccgccg tagatgatgt
     3541 cgcccagcgt ggtcatgggc gagatggagt cgaactccgc cgcgctggtc caggcggagc
     3601 cggtgaaggt gtagcgcttg gtcttggata cgacccacag ggcgaagccg tcatccggcg
     3661 ccacgaaatt ccacacgccg ttgaaccacg ttgcgatctg cgtggcgcgg ccggtccaca
     3721 ggccggtggg gctggcggcg acgatgtacg catcgccgtc gtcggggctg gccggcggcg
     3781 tggccaggtc catgtcgagc acgcggccct gcaccagcac atcgagcgcg cgcatggcgt
     3841 cattgaaggt gacttccttc tgcgcctggc tggcggacat cagcgaaaaa tcgagacgcg
     3901 gcgtttggtc gctcatggtg ggtctcagct ggagagttcg gccgtgacga tcgcggagcc
     3961 cagccaggcg tcgaccgtga acggcgtgtt ggcggcggtg gcggtgatga ccagcaccgt
     4021 gcggtagaag tcggtaggcg cgagcgccac ccagatggag ccgtcctgca gcgcggcggt
     4081 ccacgagccg gacgcatcga tcagcgcctt cagcgcgttg tagatggcgt cgtaatccag
     4141 cgcgccgccg gacacgtcgc ccgatcccgg cgtgtacgag aacacgctgg cgccgagcct
     4201 gacgtggaag gccaggccca cggcgtcgcc ggcgacggtg ccctgcaccg gcgcgaactg
     4261 cacgctcgag gcctgcgcgc ggcccgacgg caggttgggc acggtgtggc gcaccgcggt
     4321 gaggaacggg tagaagtcgg agtccgaggt tgacgacgcc aggtagtcga gcttcaccgg
     4381 cgtgggcgtg atggtgtcat caaccaccac ggccacctgc ccgctcacca gcccggtgta
     4441 gccgtactgc gcgctgatgc cgtaggcggc ca
//
LOCUS       DASZZC010000804         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_448435,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000804 DASZZC010000000
VERSION     DASZZC010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448435"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VHE37_17330"
     CDS             <1..966
                     /locus_tag="VHE37_17330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVT37352.1"
                     /translation="YKVIERAPLVTWMQAYRIVTAPPPSAGGVTLAQVFGQLEALGWH
                     GNGDVLAKHLLVESWRRAYHDRAQYLGDPDFVDVPIYRIAAHGYALALARGIDRAHAT
                     PSSTLDAQSRALHEGDHTSHFSIVDAQGNRVAATQTINTTFGCLCMAPGTGVVLNNEM
                     DDFSAAVDASNVYGLVGSKANAIAPGKRPLSSMTPSFVEGPRGLLVLGTPGGSRIITM
                     VTLGILDWISGGDTNSVVGAPRFHHQYLPDEIQFEPGAFSADEQAQLTALGHTLNPLS
                     STYGNMHAVWWDPAHDHLEAASDPRGVGRAQVVLSAKMPAPPPSK"
     gene            1039..1683
                     /locus_tag="VHE37_17335"
     CDS             1039..1683
                     /locus_tag="VHE37_17335"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT37353.1"
                     /translation="MSTSLQWLQWAQLGGPALIVLLALSIAALTIAIVKFWEFWETRI
                     WRREFVARAADAWQRRQFDQALAILQRERGPLAQVMEHALAQLRAGYDLEAAREASAH
                     YAQEKLDDARSLLRPLEVIAMLAPLIGLLGTVLGMIRVFRDLEQAGGELAAGALSGGL
                     WEALLTTAAGLAVAIVALAAFHLLDRMVERLQLDMEGALTRIYARPPAVRAHAA"
     gene            1673..2044
                     /locus_tag="VHE37_17340"
     CDS             1673..2044
                     /locus_tag="VHE37_17340"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT37354.1"
                     /translation="MQLERKPRARGTHLIITSLVDVMLVLLFYFILTAGYGKAPRELA
                     LSLAAPVAAHGSSGTLHEVRMRAPGDFLLDGAPLPLAEIQARMHGAQLALRPAPQVQL
                     QALLDALAALRKGGAQVELGT"
     gene            2041..2436
                     /locus_tag="VHE37_17345"
     CDS             2041..2436
                     /locus_tag="VHE37_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT37355.1"
                     /translation="MKLADRPRTRRPLIDMVPMINFAFLLLIFFVLAGHAGARDASRA
                     ALAHAPDAPSARAGASELVMAPDGQLSWDQAAVAPEQLLDHARAWQAAHAHTALRVHA
                     PATVSAASVIDVLQVLRAAQVAQVTLAVR"
     gene            2438..2947
                     /locus_tag="VHE37_17350"
     CDS             2438..2947
                     /locus_tag="VHE37_17350"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT37356.1"
                     /translation="MPRRTRLLLALLAALALHALLLGRWRSEDEQLPAGAGSGLGSEA
                     ADDQQDVTLAAAMPVAAGAGASATPPPAHAAPAGGGGGSGGYLARLRAHLYRYRSEPP
                     AGISARGVAQVRFTVAADGSASGVDVAESSGSRELDLQAVALVLRASPLPAPPDGLAQ
                     TLVVPVEFR"
     gene            complement(2960..4015)
                     /locus_tag="VHE37_17355"
     CDS             complement(2960..4015)
                     /locus_tag="VHE37_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37357.1"
                     /translation="MSITRRNHYDITGTVCVADPRDVGAACISLLRKLYPDADYAPVQ
                     HAFETFAELYTGTLPGFFGCETWYHDAQHSLDCALVMARLLDGYERSVADDAKLGERR
                     LRLGVIAALFHDAGYIRRQRDAEQHGAEFTLYHVKRSGDFMATYLPNIGFAAEVPVVE
                     QIVHYTGYEVALDKVVVADDRDRKLGFLLGSADVLAQMSDRCYLEKCRDFLYAEFEIC
                     GLAGLPRSSGPKPIYASPEDLLNKTAAFKDKIWEERMDGYFGSIYRYLEAHFGGKNPY
                     IAAIDRHLARLYQVSRDGMMLEGLRRRAEAVNAVPLRHIMGLPPSSELVFGNWQPPPR
                     NRKPALGVPETQRFMPT"
BASE COUNT          613 a         1405 c         1393 g          653 t
ORIGIN      
        1 tacaaggtga tcgagcgcgc gccgctggtc acctggatgc aggcgtatcg catcgtcact
       61 gcgccgccgc cgtcggccgg aggcgttacc ctggcgcagg tgttcggcca gctggaagcg
      121 ctcggctggc acggcaacgg cgatgtcctc gcaaagcact tgttggtgga gagctggcgc
      181 cgcgcctacc acgaccgcgc gcagtatctg ggtgatccgg atttcgtcga cgtgccgatc
      241 taccgcatcg ccgcgcacgg ctacgcgctg gcgttggcgc gcggcatcga ccgcgcgcat
      301 gccacgccca gcagcacgct ggatgcgcaa tcacgggcgc tacacgaggg cgaccacacc
      361 tcgcacttct ccatcgtcga cgcccagggc aaccgcgtgg cggccacgca gaccatcaac
      421 accaccttcg gttgcctgtg catggcgccc ggcaccggcg tggtgctgaa caacgagatg
      481 gatgatttct ccgccgcggt cgacgccagc aacgtctacg gcctggtcgg cagcaaggcc
      541 aatgccatcg cgcccggcaa gcggccgctg tcgtcgatga cgccgagctt cgtcgagggc
      601 ccgcgcgggc tgctggtgct gggcacgccc ggcggcagcc gcatcatcac catggtcacg
      661 ctgggcatcc tcgactggat cagcggcggc gatacgaact cggtggtcgg cgcgccacgc
      721 ttccatcatc aatacctgcc tgacgaaatc cagttcgagc ccggcgcgtt cagcgccgac
      781 gagcaggcgc aactcacggc gctcggccac acgctcaatc cgctgtcttc cacctacggc
      841 aacatgcacg cggtgtggtg ggatccggcg cacgatcacc tggaagcggc gtccgatccg
      901 cgcggcgtcg gccgcgctca agtcgttttg agcgcgaaaa tgcctgcacc gcccccctcg
      961 aaatgatgtt attaattgtt tatttattat aattttgata catattttat aacattcaaa
     1021 accggggtgc ggtaggccat gtcgacatcg ctgcaatggc tgcaatgggc acagctgggt
     1081 ggaccggcat tgatcgtgct gctggcactg tcgatcgcgg cgctgaccat cgccatcgtc
     1141 aagttctggg aattctggga gacgcgcatc tggcggcgcg agttcgtggc gcgcgcggcc
     1201 gatgcctggc agcggcgcca gttcgaccag gcgctggcca tcctgcagcg cgagcgcgga
     1261 ccgctggcgc aggtgatgga acacgcgctg gcgcagctgc gcgccggtta cgacctcgag
     1321 gccgcgcgcg aggccagcgc gcactatgcg caggaaaaac tcgacgacgc gcgcagcctg
     1381 ctgcggccgc tggaagtgat cgccatgctg gcgccgctga tcggcctgct cggcaccgtg
     1441 ctcggcatga tccgcgtgtt ccgcgacctg gagcaggccg gcggcgagct cgcggccggc
     1501 gcgctgtccg gcggcctgtg ggaggcgctg ctcaccaccg cggccgggct ggcggtggcc
     1561 atcgtcgcgc tcgccgcgtt ccacctgctg gaccgcatgg tcgagcgcct gcagctggac
     1621 atggaaggcg cgctcacgcg catctacgcg cggccgccgg cggtacgcgc gcatgcagct
     1681 tgagcgcaag ccgcgcgcgc gcggcacgca cctgatcatc acctcgctgg tcgacgtaat
     1741 gctggtgctg ctgttttatt tcatcctcac cgccggctac ggcaaggcgc cgcgcgaact
     1801 ggcgctgtcg cttgccgcgc cggtcgccgc gcacggcagc agcggcacgc tgcacgaagt
     1861 gcgcatgcgc gcaccaggcg atttcctgct cgacggcgcg ccgctgccgc tggccgaaat
     1921 ccaggcgcgc atgcacggcg cacagctggc gctgcggccc gcgccgcagg tgcaactgca
     1981 ggcgctcctc gacgcgctgg ccgcgctgcg caagggcggc gcgcaggtgg aactgggcac
     2041 atgaaactcg ccgatcgccc acgcacccgg cgcccgctca tcgacatggt gccgatgatc
     2101 aacttcgctt tcctgctgct gatcttcttc gtgctcgccg gccatgccgg tgcgcgcgac
     2161 gccagccgcg cggccctggc gcatgcgccc gatgcgccat ctgcgcgcgc cggcgcgagc
     2221 gaactggtca tggcccccga cggccagctg agctgggatc aggccgcggt cgcgccggag
     2281 caactgctcg accacgcgcg tgcatggcag gccgcgcatg cgcacaccgc gctgcgcgtg
     2341 cacgcaccgg cgacggtttc cgcggcgagc gtcattgatg tcctgcaggt gctgcgcgcg
     2401 gcacaggtgg cgcaggtcac gctggcggtg cgctgacgtg ccacgcagaa cccggctgct
     2461 gctcgcactg ctggccgcac tggcgctgca cgcgctgctg ctcgggcgct ggcgcagcga
     2521 ggacgagcaa ttgccggcgg gcgccggcag cgggctgggc agcgaggcgg ccgacgatca
     2581 acaggacgtg acgctggctg ccgccatgcc cgttgcggcg ggtgccggcg caagcgcgac
     2641 accgccgccc gcccacgcag cgcctgccgg cggcgggggc ggcagcggcg gttacctcgc
     2701 acggctgcgc gcgcacctgt accgctatcg cagcgaaccg ccggccggca tcagcgcgcg
     2761 cggcgtggca caggtgcgct ttaccgttgc cgccgacggc agtgccagtg gtgtcgatgt
     2821 agccgagagt tcgggctcac gcgagctcga cctgcaggcg gtggcgctgg tactgcgcgc
     2881 atcgccgctg ccggcgccac ccgatgggct ggcgcagacc ctggtcgttc ccgtggaatt
     2941 ccgctgaccg cagctgcggt caggtgggca tgaagcgctg tgtctccggc acgcccagtg
     3001 ccggcttgcg gttgcgcggc ggcggctgcc agttgccgaa caccagttcg ctgctgggcg
     3061 gcaggcccat gatgtggcgc agcggcaccg cgttcaccgc ctcggcgcgg cggcgcaggc
     3121 cttccagcat catgccgtcg cgcgacacct ggtacagccg cgccaggtgg cggtcgatgg
     3181 cggcgatgta cggattcttg ccgccgaaat gcgcctcgag atagcgatag atgctgccga
     3241 agtagccgtc catgcgctct tcccagatct tgtccttgaa cgcggccgtc ttgttcagca
     3301 ggtcctcggg cgaggcatag atcggcttgg gtcccgacga gcgcggcagg ccggccaggc
     3361 cgcagatctc gaactcggcg tagaggaaat cgcggcattt ctccaggtag cagcggtccg
     3421 acatctgcgc cagcacgtcg gcgctgccga gcaggaagcc gagcttgcgg tcgcggtcgt
     3481 cggccaccac gaccttgtcc agcgccacct cgtagccggt gtagtgcacg atctgctcga
     3541 ccaccggcac ctcggccgcg aagccgatat tgggcagata agtcgccatg aaatcgccgc
     3601 tgcgcttgac gtggtacagc gtgaactcgg cgccgtgctg ctcggcatcg cgctggcggc
     3661 ggatgtagcc ggcatcgtgg aacagcgcgg cgatcacgcc cagccgcagg cgacgctcac
     3721 ccagcttggc atcgtcggcc acgctacgct cgtagccgtc gagcaggcgt gccatcacca
     3781 gcgcgcagtc gaggctgtgc tgcgcatcgt ggtaccaggt ttcgcaaccg aagaagccgg
     3841 gcaacgtgcc ggtgtagagt tcggcaaagg tttcaaacgc gtgctgtacc ggcgcgtaat
     3901 ccgcatcggg atacagtttg cgcagcaggc tgatgcaggc cgcgccgaca tcgcgcgggt
     3961 cggccacgca gaccgtcccc gtgatgtcgt agtggttgcg ccgcgtgatg ctcatgcctc
     4021 tcctcccgtc cggtccccag ccggttcaac ccatggtagt cctt
//
LOCUS       DASZZC010000805        12089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_641834,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000805 DASZZC010000000
VERSION     DASZZC010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641834"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="VHE37_17360"
     CDS             complement(<1..2052)
                     /locus_tag="VHE37_17360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVT37358.1"
                     /translation="MKKMLLLSCCLLAGAAARADDALPPVEIAYQKHILGNGLTVILH
                     EDHKAPIVTANIWYHVGSKNEQPGKTGFAHLFEHLMLNGSQHYNDDFFKPLEAIGASN
                     INGTTNSDRTNYYATVPTPALDRLLWLLSDQMGYLLPAVDQPRLDEQRGVVENEKRQG
                     ENKPYGHVQETLAAALYPTGHPYSWTTIGSKEDLDHASIADVQAWFRQWYGAGNAVLV
                     VAGDIQPDEALKKIEHYFGGLSGGPVLARPQRWIAPLPAEKRVALQDRVPQTLVQIVW
                     PVPGSSEDENTLLSLAADVLGGGRNSRLYKRLVYRDRSATSAGTWFNDRELSSELAFD
                     LMVKPGGDAAAVESAAREELAKFLHDGPTAEELARVKTARYAGFVRGLESISGKSAVL
                     AESEIYGGRPDDYLRSLKVLREATPEQVRTAAVRWLGAGRVVLTVTPVPDYAPAAGDA
                     DRAHLPAVGATPNVHLPPFERATLSNGLKLVLAQRHEAPVVNMNLIVDAGQAAGAGGK
                     PGLPGYTASMLMQGTVTRDLFAISQREEELGADVGSELSDDSSNVTLSAIKARLEPSV
                     ELYADVALHPSFPADEVERLKPLRLAAIAQMMSSPQGITTNLLPQLLYRDGHPYDIAY
                     AGLGGADAIRSTTADDLRAFYRRWYRPDNATLVVAGDITLPELKALAEKYFGAWQAPP
                     EA"
     gene            2089..2685
                     /gene="ampD"
                     /locus_tag="VHE37_17365"
     CDS             2089..2685
                     /gene="ampD"
                     /locus_tag="VHE37_17365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HVT37359.1"
                     /translation="MSAKTQIPPSGSRAGWLPRVRVRPSPNCDERPDADDVSLLVIHA
                     ISLPPDRFGGRWVEDFFLNRLDISAHAYFETIRTLRVSAHLFIDRCGRVTQFVPLHRR
                     AWHAGVSSFGGRERCNDFSIGIELEGCDTQPFTAAQYRRLATAARAIMRAYPRITPAR
                     IAGHSDIAPGRKTDPGPHFDWARFRRELRSSRPPVRRA"
     gene            2682..3551
                     /gene="ampE"
                     /locus_tag="VHE37_17370"
     CDS             2682..3551
                     /gene="ampE"
                     /locus_tag="VHE37_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory signaling modulator protein AmpE"
                     /protein_id="HVT37360.1"
                     /translation="MSFVGILLALLAERALGRAPGWGEPAVFPAMVAALQRWLDLPAL
                     WRSAIAPLLLIAPPVVLVAWLRSELVHPFVELTYSSAVLLLCLGPRDLADDVHALLRA
                     RAASDTAEVERLSRALMRGPEPEPTARTLFGALFIQSHERLFGVLIWFFVCGPAGALA
                     YRLARTLPRTLRETQREATPAVHMAESIHGLLAWVPARATALLYGLGGSLDDALVEWR
                     ALENVPHEWHSHTWAILAQVPSASLSQEEADGSVAVPASLDANLGEVLRMQWRALLIL
                     LAFFAFFTTGAML"
     gene            3548..4420
                     /locus_tag="VHE37_17375"
     CDS             3548..4420
                     /locus_tag="VHE37_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HVT37361.1"
                     /translation="MRALVLLLALLASGSAVAGAQRIVTLAPHLAELVCAAGGCDYLV
                     GVVRYTDFPENAARLPQVGDAATVNVEALLALKPDLVLAWDGGTPPDTIVRLRGLGLR
                     VEAVRVRTLREIGDALWQVGTLMQTQTAAALAVKLYHHRLDQLQVRYWQKPRLTVMYQ
                     IETGPIYTVNSLSPIHEALSLCGGQNVFSDLPRIASTVSVEAVLAANPQAVFYSRQDN
                     AVAIREQWARFPQTRAQHVGAIYAVDGNLLEHQSPRMLDGIAQLCEALDDARAKYARA
                     SAASSSGAATSPTK"
     gene            complement(4374..5009)
                     /locus_tag="VHE37_17380"
     CDS             complement(4374..5009)
                     /locus_tag="VHE37_17380"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HVT37362.1"
                     /translation="MGHRLSSIVTRTGDAGTTGLTGGGRVPKAHARVQAMGEVDELNC
                     VVGALLAHRMAPGHAAMLLRIQHELFNLGGELSMPGHTLVGADEVARLEDEIEVINAK
                     LPPLKEFVLPGGNAPAAAAHLARAVARRAERALWTLHAIEPVGECALRYANRLSDLMF
                     VFARTLARARGGQEVTWNSRKAGATSSPRRRGSGSQIISSAKSQRQKKKPR"
     gene            complement(5181..7178)
                     /locus_tag="VHE37_17385"
     CDS             complement(5181..7178)
                     /locus_tag="VHE37_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT37363.1"
                     /translation="MKAWLFAGALVAPLAAFADDAQTLPPIVVTATRSPQPDNRLPAA
                     LNVITRADIATSGAQTLADVLRTAPGVQIDDFYGDGSAAEVDMRGFGDGSNATTLILV
                     DGRRLNNPDIGIPDLTSVPLKDIERIEIIQGSAGTLYGDQAVGGVINIITRAPVSEAL
                     AARAQIGGGSYGRQQASASVSQRFGGFSYRISGEGSGTDNYRDHNHLENENVLGRAGY
                     DYGSGSAYLELGFVNNRLQTPGALFAAQEAQDRRQVQPDFAGDFSNTRTGLQRLNWRQ
                     DLATGWTLETDVTHRRGDGVFRLSFTGAPATADSTQQRNVWSINPRVEGGFALPAGRA
                     LLTLGADGQLAEYQLLSPLGLQSNDQRQRDFYAQAELPLWRTLEATVGVRNARVDNKV
                     MQTSTSAFFVPVFTVPTPFHDTHTAAELGLALRPLDGLRLHARYDGNFRFAKVDEFTL
                     TAAAPASGQNLLQTQTGGTYEFGSEWTRSAYSLGLTLYQLRLKNEIAFDPVSFTNVNL
                     ARTRRSGGVLDAGWQALDALKLSAAYQHLLATNGGSEDGKRIPLAARDSGKLAATLAL
                     PHGATAYVEALAVGNRPYSGDYNNDLPNLPGYAVVNAALNRQWRSWNFAARVNNLLDH
                     RYSENGVDSPDFPPPTFTAVPREAFYPSPGRNFWITASYAP"
     regulatory      complement(7214..7411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7429..8202
                     /locus_tag="VHE37_17390"
     CDS             7429..8202
                     /locus_tag="VHE37_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVT37364.1"
                     /translation="MTSQFMTAALAAAEAASQIIRKAYRGNFSVQYKADASPVTEVDV
                     AAEQAIKQALQSRFPTHGFFGEETGRDHADAEYLWLVDPIDGTKGFVRGYPMFSVQIA
                     LMHRGELVLGVSSAPCWNGGAGEIIRAEKGQGAWLGDDRIRVSSVGEIGKATLSTGNL
                     ARLAKSPQWAELGRIIPRLHRIRGYGDFLHYHLLAQGKIDAVVESDVNILDIAALTVI
                     VREAGGRFTDLNGGEVGLETTTVLASNGPLHSLFAGLAL"
     gene            complement(8199..8861)
                     /locus_tag="VHE37_17395"
     CDS             complement(8199..8861)
                     /locus_tag="VHE37_17395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVT37365.1"
                     /translation="MRLAVFDLDHTLLAGDSDYLWGQFLCEQGRVDAAWYEAENRRYY
                     EAYVAGTLDIAAFAAFSMKPLAENEPADLYAWREQFIRDKIEPVIAAGTPALLERHRA
                     QGDTLLITTATNRFITEPIAQLLGVPHLIATDPEMRDGRYTGRLAGLANFQSGKVRRL
                     ELWREQQAQKFAHMTCYSDSRNDIPLLEMADSAVAVDPDATLRAEAGKRGWPVISLRD
                     VL"
     gene            8933..9436
                     /locus_tag="VHE37_17400"
     CDS             8933..9436
                     /locus_tag="VHE37_17400"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HVT37366.1"
                     /translation="MIDRDGFRPNVGIILASSSGQVLWAKRAGQDAWQFPQGGINRGE
                     SPEEAMYRELNEELGLDRTHVQVLGVTQDWLHYRLPQRFIRRRRGRVCIGQKQKWFAL
                     RLIGAESLVRFDVTLQAEFDGWRWVDYWHPLAEVIEFKREVYHKALHELAPLLGLGVP
                     APPVRTA"
     gene            complement(9423..9839)
                     /gene="hemJ"
                     /locus_tag="VHE37_17405"
     CDS             complement(9423..9839)
                     /gene="hemJ"
                     /locus_tag="VHE37_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HVT37367.1"
                     /translation="MLWVKAFHIMFVVAWFAGLFYLPRLFVYHCEVSDDDGYRRFCLM
                     EKRLMTMTTIGMIGTWLLGLSLLVITPAFVHMGWLHAKLTLVLILSGYHGWLKTRVRA
                     FEHKRNDKDARFYRLMNEVPSLLLVLIVLLVVVKPF"
     gene            9908..10939
                     /gene="argC"
                     /locus_tag="VHE37_17410"
     CDS             9908..10939
                     /gene="argC"
                     /locus_tag="VHE37_17410"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HVT37368.1"
                     /translation="MVKVGIVGGTGYTGAELLRILARHPRARVALLTSRQEAGRRVDS
                     WSPHLRGCFDLSFTEPDARALSQCEAVFFATPHGTAMRMAPELVRAGVRVIDLSADFR
                     LRDPAVFQQWYGMPHSATDLLAEAVYGLPEMARERIRQARLLANPGCYPTAVQLGLIP
                     LLEAGIVSTDNLIADCKSGVSGAGREAKPHLLFGEASDTMKAYGLPGHRHAPEIEQGL
                     SAARGEPVRVTFVPHLTPMIRGIHATLYARLSDPSADLQALFEKRYRGEPFVDVLPAG
                     ELPETRSVRGANHCRIAVQKSADGRHAIVLSVIDNLVKGAAGQAVQNFNLMLGLDETL
                     GLESVPLSP"
     gene            10946..11686
                     /locus_tag="VHE37_17415"
     CDS             10946..11686
                     /locus_tag="VHE37_17415"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HVT37369.1"
                     /translation="MKHKIVITRDRPWFKAGIIAGSAFAIAVSGFALYRYTRATTVSD
                     FERAQTEVEQLRDERRSLTRDLRAARTQIDQLKDQVVYAQRSGEIDSQACGTVKASLS
                     GLQAEVADLREQLAFYRGIVSPDLSRAGVRVYEFKVDKSTTSNAYRYELVLIQSVRHD
                     RQVGGRVQIDVEGTKDGAAQTFKLDDAALGGAKNLQFSFKYFEEFNGEFHLPAGFRPV
                     RVVVTLATDQADAPDVKEQFDWDKVTKV"
     gene            11770..>12089
                     /locus_tag="VHE37_17420"
     CDS             11770..>12089
                     /locus_tag="VHE37_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVT37370.1"
                     /translation="MVDTLIGKQTEILGDVRFSGGLHVDGKIKGKVLAEAGNKGASLS
                     VSENGAVEGDVRVPNIVLNGKVTGDVHATERIHLAAKARVNGNVYYKIIEMEGGAEIN
                     GQLVR"
BASE COUNT         1862 a         3924 c         4221 g         2082 t
ORIGIN      
        1 cgcctccggc ggcgcctgcc acgcgccgaa atatttttcc gccagcgcct tcagctccgg
       61 cagcgtgatg tcgccggcca ccaccagcgt ggcgttgtcg ggccggtacc agcgccggta
      121 gaacgcgcgc aggtcgtccg ccgttgtcga gcggatcgca tcggcaccgc ccaggccggc
      181 gtacgcgatg tcgtacggat gcccgtcgcg gtacaacagc tgcggcagca ggttggtggt
      241 gatgccttgc ggcgacgaca tcatctgcgc gatggccgcc agccgcagcg gcttgagccg
      301 ctccacctcg tcggcgggga acgaaggatg cagcgccacg tcggcgtaca gttccaccga
      361 gggctcgagc cgcgccttga tcgccgacag cgtgacgttg gaggaatcgt cggacagttc
      421 gctgccgacg tcggcgccca gttcttcctc gcgctggctg atggcgaaca ggtcgcgcgt
      481 gacggtgccc tgcatcagca tgctcgcggt gtatcccggc agtccgggct tgccaccggc
      541 gccggcggcc tggccggcat cgacgatcag gttcatgttc accaccggcg cctcgtgccg
      601 ctgcgccagc accagcttga gaccgttgga gagcgtggcg cgctcgaacg gcggcaggtg
      661 cacgttgggc gtggcgccta ccgccggcag gtgcgcgcgg tcggcatcgc ccgctgccgg
      721 cgcgtagtcg ggcaccggcg tcaccgtcag caccacgcgt cccgcgccga gccagcgcac
      781 agccgctgtg cgcacctgct cgggcgtggc ctcgcgcagc accttgagcg aacgcaggta
      841 gtcgtcgggc cggccgccgt agatctcgct ttccgccagc acggcggact tgccggagat
      901 gctctcgagt ccgcgcacga agccggcata gcgcgcggtc ttcacgcgcg cgagttcttc
      961 cgcggtggga ccgtcatgca ggaacttcgc cagctcttcg cgtgcggcgc tttccaccgc
     1021 ggccgcatcg ccgcccggct tcaccatcag gtcgaaggcc agctcgctcg acaactcgcg
     1081 gtcattgaac caagtgccgg cgctggtcgc gctgcggtcg cggtagacca ggcgcttgta
     1141 cagccggctg ttgcggccgc caccgagcac gtcggccgcc agcgacagca gcgtgttctc
     1201 gtcttcgctg gagcccggca ccggccacac gatctgcacc agcgtctgcg gcacgcggtc
     1261 ctgcagcgcc acgcgttttt ccgcgggcag cggcgcgatc cagcgctgcg gccgcgccag
     1321 caccgggccg ccgctcagcc cgccgaagta gtgctcgatc ttcttcagcg cctcgtcggg
     1381 ctggatgtcg cccgccacca ccagcacggc gttgccggcg ccgtaccact ggcggaacca
     1441 ggcctgcacg tcggcgatgg aggcgtggtc gagatcttcc ttcgagccga tcgtggtcca
     1501 cgaatacgga tggccggtgg ggtagagcgc tgcggccagc gtttcctgca catggccgta
     1561 gggcttgttc tcgccctggc gcttttcgtt ctccaccacg ccgcgctgct cgtcgaggcg
     1621 cggctggtcc acggccggca gcaggtagcc catctggtcc gacagcagcc acagcagccg
     1681 gtccagcgcc ggcgtcggca cggtggcgta gtagttggtg cggtcggagt tggtggtgcc
     1741 gttgatgttc gacgcgccga tcgcctccag cggcttgaag aagtcgtcgt tgtagtgctg
     1801 cgagccgttg agcatcaggt gctcgaacag gtgggcgaag ccggtcttgc ccggctgctc
     1861 gttcttgctg ccgacgtggt accagatgtt ggcggtgacg atcggcgcct tgtgatcctc
     1921 gtgcaggatc accgtcaggc cgttgccgag gatatgcttc tggtaggcga tctccaccgg
     1981 cggcagcgcg tcatccgcgc gcgcggcggc gcccgccagc aggcagcacg agaggagcag
     2041 cattttcttc atgttcgatc cccagagcgg gcgcgcagtg tcgcggccgt gagcgctaag
     2101 acgcaaattc cgccaagtgg ttcccgtgcg ggctggctgc cgcgtgtgcg cgtgcggcct
     2161 tcgcccaact gcgacgagcg gccggacgcg gatgacgtca gcctgctggt gatccacgcc
     2221 atttcgctgc caccggaccg gttcggcggc cgctgggtgg aggatttctt cctcaaccgg
     2281 ctcgatattt cggcgcatgc gtatttcgag acgatcagga cgctgagggt ttcggcgcat
     2341 cttttcatcg accgctgcgg gcgcgtcacc cagttcgtgc cgctgcaccg gcgcgcctgg
     2401 catgcgggcg tgtcgagctt cggcggccgc gagcgttgca acgacttttc catcggcatc
     2461 gagctggaag gctgcgacac gcagccgttc actgccgcgc agtaccggcg gctggccacg
     2521 gcggcacgcg ccatcatgcg cgcgtatccg cgcatcacgc cggcgcgcat cgccgggcac
     2581 agcgacatcg cgcccgggcg caagaccgat ccgggtccgc atttcgactg ggcgcgcttt
     2641 cggcgagaat tgcgctcttc ccggccacct gtgcggcgcg catgagtttt gtcggcatcc
     2701 tgttggccct gctcgcggaa cgcgcactcg gccgcgcgcc cggctggggc gagcctgcgg
     2761 tgttccccgc catggtcgcc gcgctgcagc gctggctcga tctgccggcg ctgtggcgtt
     2821 cggccattgc gccgctgctg ctgatcgcgc cgccggtagt gctggtagcc tggctgcgca
     2881 gcgagctggt gcatcctttc gtggaactga cctattcgag cgccgtgctg ctgctgtgcc
     2941 tgggcccgcg cgacctggcc gacgacgtgc acgcgctgct gcgggcgcgc gccgccagcg
     3001 acaccgccga ggtggagcgg ctctcgcgcg cgctgatgcg cggccccgag ccggagccga
     3061 ccgcgcgcac gctgttcggc gcgctgttca tccagtcgca cgagcgcctg ttcggcgtgc
     3121 tgatctggtt cttcgtctgc gggcccgcgg gcgcgctggc gtaccggctg gcgcggacgc
     3181 tgccgcggac gctgcgcgag acccagcgcg aggcgacgcc ggcggtgcac atggccgaat
     3241 ccatccacgg cctgctggcc tgggtgccgg ctcgcgccac cgcgctgctc tatggcctgg
     3301 gcggcagcct cgacgacgcg ctggtggaat ggcgcgcgct ggagaacgtg ccgcacgaat
     3361 ggcattccca cacctgggcc atcctggctc aggtgccgtc ggccagcctg tcgcaggaag
     3421 aggccgatgg cagcgtggcg gtgccggcct cgctcgatgc caacctgggc gaagtgctgc
     3481 gcatgcaatg gcgcgcgctg ctgatcctgc tggcgttctt cgctttcttc acaaccggcg
     3541 ccatgctgtg agggcgctgg tcctgctcct cgcgctgctg gcgagcggca gcgccgttgc
     3601 cggggcccag cgcatcgtca cgctggcgcc acacctggcc gaactggtgt gcgccgcggg
     3661 cggctgcgac tacctggtcg gcgtggtgcg ctataccgac ttcccggaga acgctgcgag
     3721 attgccgcag gtgggtgacg cggccacggt gaatgtcgag gcgctgctgg cgctcaagcc
     3781 cgacctggtg ctggcctggg atggcggcac gccgcccgac accatcgtgc gcctgcgcgg
     3841 gctgggcctg cgcgtggaag cggtgcgcgt gcgcacgctg cgcgagatcg gcgatgcgct
     3901 ctggcaggtc ggcacgctga tgcagacgca gaccgcggcg gcgctggcgg tgaagctcta
     3961 tcaccatcgc ctggaccagt tgcaggtgcg ttactggcag aagccgcggc tcaccgtgat
     4021 gtaccagatc gagaccggcc cgatctatac ggtgaattcg ctgagcccga tccacgaggc
     4081 gctgtcgctg tgcggcggcc agaacgtgtt ctccgacctc ccgcgcatcg ccagcacggt
     4141 gagcgtggaa gcggtgctgg cggccaatcc gcaggcggtg ttctattcgc gccaggacaa
     4201 cgcggtggcc atccgcgagc aatgggcgcg ttttccgcag acgcgcgcgc agcatgtcgg
     4261 cgcgatctac gcggtggacg gcaacctgct ggagcaccag tcgccgcgca tgctcgacgg
     4321 catcgcgcag ctgtgcgagg cgctggacga cgcgcgcgcc aagtacgcgc gggctagcgc
     4381 ggcttcttct tctggcgctg cgacttcgcc gacgaaataa tttgcgaacc ggatccccgc
     4441 ctgcgcgggg atgacgtggc gccagccttg cggctgttcc acgtcacttc ctggcctccg
     4501 cgcgcgcggg ccagcgtgcg tgcgaacacg aacatcaggt cggacagccg gttggcgtag
     4561 cgcagcgcgc attcgccgac cggctcgatc gcgtgcagcg tccacagcgc gcgctcggcg
     4621 cggcgcgcca ccgcgcgcgc caggtgcgcg gccgcggcag gcgcattgcc gccgggcagc
     4681 acgaattcct tgagcggcgg cagcttcgcg ttgatcacct cgatctcgtc ttccaggcgc
     4741 gccacttcgt cggcgccgac cagcgtgtgc ccgggcatcg acagctcgcc gcccaggttg
     4801 aacaactcgt gctggatgcg cagcagcatg gccgcgtgcc cgggggccat cctgtgtgcg
     4861 agcagcgcgc cgaccacgca gttgagctcg tccacttcgc ccatggcctg cacccgcgcg
     4921 tgcgccttgg gcacgcggcc gccgccggtc aggccggtgg tgccggcgtc gccggtgcgc
     4981 gtgacgatgc tgctgagtcg gtggcccatg gctggcggaa atcttcgttg gagaaagtca
     5041 aaaagcagtc tagcagcgcg aaaaatgcct ccaccgcacc ccggcttggt atgttataaa
     5101 aaattatata aaactaaaat taataaataa tttataacac aaatacatgg ggtgcggtgg
     5161 agcggatttt gcgctcgccg ctacggcgcg tagctcgcgg tgatccagaa attgcgtccg
     5221 ggcgagggat agaacgcctc gcgcggcacc gccgtgaacg tgggcggcgg aaagtcgggc
     5281 gagtccacgc cgttctccga gtagcggtga tcgagcaggt tgttcacccg cgctgcgaag
     5341 ttccagctgc gccactgccg gttgagcgcg gcgttgacca ccgcgtaacc gggcaggttg
     5401 ggcaggtcgt tgttgtagtc gccggagtat ggacggttgc cgactgccag cgcctcgacg
     5461 taagccgtgg cgccatgcgg cagcgcgagc gtggcggcga gcttgccgct gtcgcgtgcg
     5521 gccagcggga tgcgcttgcc gtcctcgctg ccgccgttgg tcgccagcag gtgctggtag
     5581 gcggcggaca gcttcagcgc gtcgagcgcc tgccagccgg cgtcgagcac gccgcccgag
     5641 cggcgcgtgc gcgcgaggtt gacgttggtg aagctgaccg ggtcgaaggc gatctcgttc
     5701 ttcagccgca gctggtacag cgtgaggccg agcgagtacg cgctgcgcgt ccactcgctg
     5761 ccgaactcgt aggtgccgcc ggtctgcgtc tgcagcaagt tctgccctga cgccggcgcc
     5821 gccgccgtca gggtgaactc gtcgaccttg gcgaaacgga agttgccgtc gtagcgcgcg
     5881 tgcaggcgca ggccatcgag cgggcgcagg gccagcccaa gctcggcggc ggtatgcgtg
     5941 tcgtggaacg gcgtgggcac ggtgaagacc ggcacgaaga acgcgctggt cgaggtctgc
     6001 attaccttgt tgtccacgcg tgcgttgcgc acgccaacgg tggcttccag cgtgcgccac
     6061 agcggcagct cggcctgcgc gtagaaatcg cgctggcgct ggtcgttgct ctgcaggccc
     6121 agcggcgaca gcagctggta ttccgccaac tgcccgtcgg cacccagcgt gagcagcgcg
     6181 cggccggccg gcagcgcaaa gccgccttcc acgcgcggat tgatcgacca tacgttgcgc
     6241 tgctgggtgg agtcggctgt ggccggcgcg ccggtgaagc tcagccggaa cacgccgtcg
     6301 ccgcggcggt gggtgacgtc ggtttccagc gtccagccag tggccagatc ctgccgccag
     6361 ttgagccgct gcaggccggt gcgggtattg gagaagtcac cggcgaagtc gggctgcacc
     6421 tggcggcggt cctgcgcttc ctgcgcggcg aacagcgcgc cgggcgtctg caggcggttg
     6481 ttgacgaatc ccagttcgag atacgcgctg ccgctgccat agtcatagcc ggcgcggccg
     6541 agcacattct cgttctcgag atggttgtgg tcgcggtagt tgtcggtgcc gctaccttcg
     6601 ccggagatgc gataggaaaa gccgccgaag cgctggctga cgctggcgct ggcttgctgg
     6661 cggccatagc tgccgccgcc gatctgcgcg cgcgcagcga gtgcctcgga caccggtgcg
     6721 cgcgtgatga tgttgatcac gccgccgacc gcctggtcgc cgtatagcgt gccggcgctg
     6781 ccctggatga tctcgatgcg ctcgatgtcc ttcagcggga cgctggtgag atcggggatg
     6841 ccgatgtcgg ggttgttcag ccggcggccg tcaaccagga tcagggtggt ggcgttgctg
     6901 ccgtcaccga agccgcgcat gtcgacctcg gcggcggagc cgtcgccgta gaaatcgtcg
     6961 atctgcacgc cgggcgcagt gcgcagcacg tcggccagcg tctgcgcgcc gctggtggcg
     7021 atgtcggcgc gggtgatcac gttcagcgcc gccggcagcc ggttgtccgg ttgcggcgag
     7081 cgggtggcgg tgaccacgat gggcggaagg gtttgtgcgt cgtccgcgaa tgcggcgagt
     7141 ggcgcgacga gcgcgccggc gaacagccag gctttcatgt catgtctcct gcgtcttgcg
     7201 tccccgcaag cgcgtgagtg ggtgggcgcc aggccggtct ccggactcgg gagcgtttcg
     7261 agcgtcgcct tcccgaagca tgctgcgctc cagtggccga tgacgccgtc ttctccctta
     7321 ccgttgcggg ggcagtgtcc gccttgcacg gacttcccgt ttaaccgcgg gcgctcaaca
     7381 cgcacgcggc acctgacggc gcgcattgta cgcgagctaa aattacgcat gacttcccaa
     7441 ttcatgaccg ccgcgctggc cgctgccgaa gccgccagcc agatcatccg caaggcgtac
     7501 cgcggcaatt tctcggtgca gtacaaggcc gacgccagtc ccgtgacgga agtggacgtg
     7561 gcggcggaac aggccataaa gcaggcgctg caatcgcgct ttccgacgca cggatttttt
     7621 ggcgaggaga ccgggcgcga ccacgccgat gccgagtacc tgtggctggt cgatccgatc
     7681 gacggcacca agggcttcgt gcgcggttac ccgatgttct cggtgcagat cgcgctgatg
     7741 catcgcggtg agctggtgct cggcgtgtcg agcgcgccgt gctggaatgg cggcgccggc
     7801 gaaatcatcc gcgcggaaaa aggccagggc gcgtggctgg gcgacgaccg catccgcgtg
     7861 agctcggtcg gcgagatcgg caaggccacg ctgtccaccg gcaatctcgc gcgcctggcg
     7921 aaatcgccgc agtgggccga gctgggccgc atcattccgc ggctgcatcg catccgcggc
     7981 tacggcgatt tcctgcacta ccacctgctc gcccaaggca agatcgacgc ggtggtggag
     8041 tcggacgtca acatcctgga catcgccgcg ctgacggtga tcgtgcgcga ggccgggggc
     8101 cggtttaccg atctcaacgg cggcgaggtc gggctggaga cgacgacggt gctggccagc
     8161 aacggcccgc tgcattcgct gttcgcgggg ctcgcgctct agagtacgtc gcgcagcgag
     8221 atcaccggcc agccgcgctt gccggcttcg gcgcgcagcg tggcatcggg gtcgaccgcg
     8281 accgcgctgt cggccatttc cagcagcggg atgtcattgc gtgaatcgct gtagcaggtc
     8341 atgtgcgcga atttctgcgc ctgctgctcg cgccacagct ccaggcgccg caccttgccc
     8401 gactggaagt tggccagtcc ggcgaggcgg ccggtgtagc ggccgtcgcg catttccgga
     8461 tcggtggcga tcaggtgcgg cacgcccagc agctgcgcga tcggctcggt gatgaaccgg
     8521 ttggtggcgg tggtgatcag caacgtgtcg ccctgcgcgc ggtggcgttc gagcagcgcg
     8581 ggcgtgccgg cggcaatgac cggctcgatc ttgtcgcgaa tgaactgctc gcgccaggcg
     8641 tacaggtcag cgggttcgtt ctccgccagc ggcttcatcg agaaggcggc aaaggcggcg
     8701 atgtcgagcg tgccggccac gtaggcctcg tagtagcgcc ggttctccgc ctcgtaccag
     8761 gcggcatcca cgcggccctg ctcgcacagg aactggcccc acaggtaatc gctgtcgccg
     8821 gcgagcaggg tgtggtcgag gtcgaagacg gcaaggcgca tggggcgcgc agtttgcccc
     8881 agcgtgcggg gctgtggaag aattttccgc aaatttactg gcagcagcag ccgtgatcga
     8941 tcgggacggc ttccggccga atgtaggcat catcctcgcg agctccagcg gacaggtgct
     9001 gtgggccaag cgcgccgggc aggacgcctg gcagtttccc cagggcggca tcaaccgcgg
     9061 cgagtcgccg gaagaggcga tgtaccgcga gctcaacgag gaactcggcc tcgaccgcac
     9121 gcacgtgcag gtgctcggcg tgacgcagga ctggctgcac taccgcctgc cgcagcgctt
     9181 catccgccgc cgccgcgggc gcgtgtgcat cggccagaag cagaagtggt ttgccttgcg
     9241 cctgatcggc gcggagtcgc tggtgcgctt cgacgtgacg ctgcaggccg agttcgatgg
     9301 ctggcgctgg gttgattact ggcacccgct ggccgaggtg atcgaattca agcgcgaggt
     9361 ctaccacaag gcgctgcacg aactcgcgcc gctgctgggt ctgggcgttc ccgctcctcc
     9421 ggtcagaacg gcttgaccac caccagcagc acgatcagca ccagcagcag gctcggcacc
     9481 tcgttcatca gccggtagaa gcgcgcgtcc ttgtcattgc gcttgtgctc gaacgcgcgc
     9541 actcgcgtct tcagccagcc gtgataaccg gagaggatca gcaccagcgt gagcttggcg
     9601 tgcagccagc ccatgtgcac gaaagccggc gtgatgacca gcagggaaag gccgagcagc
     9661 caggtgccga tcatgccgat ggtggtcatg gtcatcagcc gcttctccat caggcagaag
     9721 cgccggtagc cgtcgtcgtc gctcacctcg cagtgataga cgaacagccg cggcaggtag
     9781 aacaggcccg cgaaccaggc aacgacgaac atgatgtgaa aggctttgac ccagagcatg
     9841 aggaaaaggc tcgaaattgg agggaacagc acctattatc gctgccccgt cgtgcaggtg
     9901 atgtgtcatg gtcaaggtgg gtatcgtcgg cggcaccggc tacaccggtg cggaactgtt
     9961 gcgcatcctg gcgcggcacc cgcgtgcgcg cgtggccctg ctgacctcgc gccaggaagc
    10021 cgggcgccgg gtcgactcct ggtccccgca cctgcgcggt tgtttcgatc tgagtttcac
    10081 cgagcccgat gccagggcgc tttcccagtg cgaggcggtg ttctttgcca cgccgcacgg
    10141 caccgcgatg cgcatggcac cggaactggt gcgcgcgggc gtgcgcgtca tcgacctgtc
    10201 ggcggatttc cgtttgcgcg accctgcggt gttccagcag tggtacggca tgccgcacag
    10261 cgccaccgac ctgctggccg aagcagtcta cggcctcccg gaaatggcgc gcgagcggat
    10321 caggcaggcg cgcctcctgg cgaacccggg ctgctatccc acggccgtcc agctgggact
    10381 gattcccctg ctcgaggccg gcatagtttc caccgacaac ctgattgccg actgcaagtc
    10441 tggcgtcagc ggtgctggcc gtgaggccaa gccgcatctg ctatttggcg aggcctcgga
    10501 cacgatgaag gcctatggcc tgcccggaca caggcacgcg ccggaaatcg agcaggggct
    10561 cagcgctgcg cgcggcgaac ccgtgcgagt cacgttcgtg ccgcatttga cgccgatgat
    10621 ccgcggcatc catgccacgc tgtatgcgcg gctttccgac ccgtcggccg acctgcaggc
    10681 cctgtttgag aagcgctatc ggggcgagcc gttcgtcgat gtgcttcccg ccggcgagtt
    10741 gcccgagacg cgctcggtgc gcggcgccaa tcattgccgc atcgccgtgc agaaatcggc
    10801 cgatggacgt catgccatcg tgctctcggt gatcgacaat ctggtcaagg gcgcggctgg
    10861 tcaggcggtg cagaacttca acctcatgct tgggctggac gaaactctgg gtctggaatc
    10921 ggtcccgctg agtccttgag cgcggatgaa gcacaagatc gtcatcacgc gcgaccggcc
    10981 ctggttcaag gccggcatca tcgccggcag cgccttcgcc attgccgtca gcgggttcgc
    11041 gctgtatcgc tacacgcgcg caaccacggt gtctgatttc gagcgggcac agacggaagt
    11101 ggagcagttg cgtgacgagc gacgcagcct gacacgcgat ttgcgtgcgg cgcgcacgca
    11161 aatcgatcaa ttgaaagatc aggtggtgta cgcgcagcgt tcgggcgaga ttgacagtca
    11221 ggcgtgcggc acggtgaagg cctcgctgag cggactgcag gcggaagtgg ctgatttgcg
    11281 cgaacaactc gcgttctacc gaggaattgt ctcgcccgat ctgtcacgcg ccggtgtccg
    11341 cgtctatgaa ttcaaagtgg ataaatcaac cacttccaac gcctaccgtt atgaattagt
    11401 cttgattcaa tccgtgcgtc atgaccggca agtcggcgga cgggtgcaga ttgatgtgga
    11461 aggcaccaag gacggcgcag cgcagacgtt caagctcgac gatgcagcct tgggtggtgc
    11521 caagaacctg cagttctcct tcaagtactt tgaagagttc aacggcgagt tccatctgcc
    11581 ggcgggtttc cgcccggtgc gggtagtggt cactctggca accgaccagg ccgatgctcc
    11641 agatgtgaag gagcagttcg actgggacaa ggtgaccaag gtctagggta gtttcgactt
    11701 cagggggaac agatgatgag caagatgttt ggcggtagcg gacccagtgc tccaaaagga
    11761 aatgactccg tggtcgatac cctcattgga aagcagactg aaatcctggg tgatgtgcgc
    11821 ttctcgggcg gcctgcacgt ggatggcaag atcaagggca aggtgctggc cgaggccggc
    11881 aacaagggcg catcgctgtc cgtcagcgag aacggcgcgg tggaaggcga cgtgcgcgtg
    11941 ccaaacatcg ttctgaacgg caaggtcacc ggcgacgtgc acgcaaccga acgcatacat
    12001 ctggcggcca aggcgcgcgt gaacggcaac gtctactaca agatcatcga gatggaaggc
    12061 ggcgccgaga tcaacgggca gctggtccg
//
LOCUS       DASZZC010000806         5979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_892614,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000806 DASZZC010000000
VERSION     DASZZC010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892614"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1523)
                     /locus_tag="VHE37_17425"
     CDS             complement(48..1523)
                     /locus_tag="VHE37_17425"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVT37371.1"
                     /translation="MTSDRPLPFVVNLHEAAARLEQVSSVSLPDGGELHVALPLAYAR
                     QPRERFPLLVVPDARTLFGSAVEMTRLMTQTRELRPCIVAGLDDALASAADARAFCAR
                     LEQLVLPRLRQQYRISEIVLFGPLADEAATTHAILANVPGVDHYIQGGRDADGSLPAL
                     VRGVRARLSSGRRYGDEMATMARPLVAGTLGLLAPLIGRLRRKPPDVPENPQLLRSRL
                     MQRDFEIFVSLPASAARSPQRRYPVLLALDANIEFSTVAETAARMAAANEIAEVAVVG
                     IGVPRVLGPVEFGFRRFEEFCPPADGYQWDDELARVFRSLFALRGQDARTRVGQAAQF
                     LGFITGELLPLLSRTLPIDVSQAGLLGHSAGGTFVAYALCQPGAPFTHYACISPGVGM
                     AGSWLLRRRAGDKRVAAGAQSVFLSIGSEERDNRFNQMAGIPDTPALAQSLRDARQLK
                     VVERCFEGETHSSTYPLAVVHALRVAYAGAARATARQAGAA"
     gene            complement(1553..4063)
                     /locus_tag="VHE37_17430"
     CDS             complement(1553..4063)
                     /locus_tag="VHE37_17430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT37372.1"
                     /translation="MTAAVLLLVSAGVAQAQDDGTGAVPSSAPGPSAPAADTAPAEAT
                     PASIPADQPVAGTAAESSATPAEPAPAPATIAVQDLHPESANAAADDGTQRLDTVEVT
                     ASYRKENIQDVAGSAHAFGGRALDKAGATGMQDYLVQVPSVSLQKSGNGTAKIAMRGI
                     SNVNTSDLGYADGSPTTGVYLDDVAIQGSGVFPDLNIYDLARIEVLKGPQGTLYGEGS
                     MGGAIKMITREPSFNEWELRGNSSYAITEQGSPSYDTRLAVNAPLWHDRVGLRVVGTA
                     RVTGGFVDYVNLNRPDADQERADSVRGLLRWQVSENLQADLLHLQDFSHKEQFPVVDE
                     GKQEELTNSRVEDQYAKTRFAIDALTLRWTLPFANLTSVSALYDTDKDTRRRTPVLQT
                     LINGQFLTLGLGPAPDIFSNASTRVVTGLHSFSQELRLVSFGGERFDWIAGAFYRDRH
                     QSFDEAKREDTVPTIDPTGGLVTGLLGGFYPVQGEQEHGTGDEAFTQEAAYGELKWNA
                     IPDTLDITGGLRYFSEEINFFFDTHLYGLEAYLIGSDPTLIDPQTKTARVDIQQRMKS
                     SGFLPKLSAAWHFNQDHMAYLTISRGFRSGASNIYSALHSGPPIIRPDYVLNMEIGSK
                     STWLDGRLTSNIAAYHIDWKDMQGTVLGTAQLGLVPVPNFAHLDNAGDAVVYGAEASL
                     AWLPFRSVTLLGNAGYNNGHVTRSEDSTIVQPGGQLPNMPRWTLSSTASVNFDLPFGL
                     TGDASTTLAFIGSQYVVFQPTPQKDKLGNDIDFGDLTVPSYSLLKANVSAGTGQLRVQ
                     LYGDNLLNRHAITAISGPTPQYTVIPPRTVGLRLSYEL"
     gene            4172..4843
                     /locus_tag="VHE37_17435"
     CDS             4172..4843
                     /locus_tag="VHE37_17435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT37373.1"
                     /translation="MGKTNQSAVRPAAPRAQPARTRLTRAEAKAQRTQQLLDAAWELF
                     CERGYEALTIDHVAERAGYSRMPIYSLFGDKQNLFFELWRAKTAQLTQVLIGGFAPGA
                     SLRRNIRQLAEVLASRPMVEAPMHAESMFFVVQTIALSRPDLEEKLQALARKVIDDFA
                     GMIRRAALEKGDVLRGTPEVVAAHMVAHINGMANVKFQTGGQYTKARDLVAIFNAIAF
                     KSPDE"
     gene            4836..>5979
                     /locus_tag="VHE37_17440"
     CDS             4836..>5979
                     /locus_tag="VHE37_17440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HVT37374.1"
                     /translation="MSDVPRLSAATLAAAAPDVEPPRYARRSERRICHIGAGRFHRAH
                     QAYYLHRLLQQGDGEGWGICAIGLKRADAEVHARLQPQDQLYSLWELDGDKRRGTVVG
                     SLMQWVDAAADADAAIAVMADPATHIISLTVTESGYCLDARGALDCAHPDIAHDLAGG
                     APRSAPGLIVRALAARRAAGGSGVTLMSCDNLLENGKVLRACMLHFAQRVDAALERWI
                     ERECSFPCSMVDRITPAADDARSAQLSRELGLRDDALVFCEPWQQWVIEDHFVAGRPA
                     FEKAGAVFSMRVRDYERMKVGMLNGGHSALSHVGLLLGHTRVHEALAVPELRLWLAAY
                     MDDVAQVLPPLPGVNYLEYRDALLARFSNPAIEDRLARLALDTTAKY"
BASE COUNT          935 a         1943 c         2106 g          995 t
ORIGIN      
        1 cgcgtcgccg cccagggtgc tgcgcagctg ttcgagtatc gcgctcatca tgcagctccg
       61 gcctggcgcg cggtcgcgcg cgcggcgcca gcgtatgcca cgcgcagcgc atgcaccact
      121 gccagcgggt aggtgctgga atgcgtttcg ccttcgaagc agcgctcgac caccttcagc
      181 tgccgcgcgt cgcgcagcga ctgcgccagc gccggcgtgt ccgggatgcc ggccatctgg
      241 ttgaagcggt tgtcgcgctc ctcgctgccg atcgacagga acacgctctg cgcgccggcg
      301 gccacgcgct tgtcgccggc acgccggcgc agcagccagc tgccggccat gcccacgccg
      361 gggctgatgc aggcatagtg ggtgaacggc gctcccggct ggcacagcgc gtaggccacg
      421 aaggtgccgc cggcggaatg gcccagcaac ccggcctggc tgacatcgat cggcagcgtg
      481 cggctgagca gcggcagcag ttcaccggtg atgaatccca ggaactgcgc cgcctgcccc
      541 acgcgcgtgc gcgcgtcctg gccgcgcaac gcgaacagcg agcggaacac gcgcgccagt
      601 tcgtcatccc actggtagcc gtcggccggc gggcagaatt cctcgaagcg gcggaagccg
      661 aattccaccg gccccagcac gcgcggcacg ccgatgccga ccacggccac ttccgcgatt
      721 tcgttagctg ccgccatgcg cgccgcggtc tccgctaccg tggaaaattc gatgttggcg
      781 tccagcgcca gcaggaccgg gtagcggcgc tgcgggctgc gcgccgcgga ggccggcagg
      841 ctgacgaaga tctcgaagtc gcgctgcatc aggcgcgagc gcagcagctg agggttctcc
      901 gggacgtccg gcggcttgcg ccgcagcctg ccgatcagcg gcgcgagcag gcccagcgtg
      961 ccggcgacca gcggccgcgc catggtggcc atctcgtcgc cgtagcggcg cccgctggac
     1021 agcctcgcgc gcacgccgcg caccagcgcc ggtagtgacc catcggcatc gcggccgccc
     1081 tggatgtagt gatccacgcc cggcacgttc gccaggatgg catgtgtagt ggcggcctcg
     1141 tcggcaagcg gcccgaacag cacgatttcg ctgatgcggt actgctgccg caggcgcggc
     1201 agcaccagct gttcgagccg cgcacagaag gcacgggcgt cggcggccga tgccagcgcg
     1261 tcgtccagcc cggcgacgat gcacggccgc agctcgcggg tctgcgtcat cagccgcgtc
     1321 atctcgacgg ccgaaccaaa cagcgtgcgg gcgtcgggca ctaccagcag cgggaaacgt
     1381 tcgcgtggtt gccgggcgta ggccagcggc agcgcaacat gtaattcgcc gccatccggc
     1441 agcgacacgc tgctcacctg ttccaggcgc gcggcggctt cgtgcaggtt gacgacgaag
     1501 ggcaggggac gatcagaggt catggtgggt caggaaaccg atggtgctgg ggtcagagtt
     1561 cgtacgacag tctcaggccg acggtacgcg gcgggatgac ggtgtattgc ggcgtgggac
     1621 cggaaatcgc ggtgatcgcg tgccggttga gcaggttgtc gccgtacagc tgcacgcgca
     1681 gctggccggt gccggcgctg acattggcct tgagcaggct gtagctcgga acggtgagat
     1741 cgccgaagtc gatatcgttg cccagcttgt ccttctgcgg ggtcggctgg aacaccacgt
     1801 actgcgagcc gatgaaagcc agggtggtgc tggcgtcgcc ggtcaggccg aagggcaggt
     1861 cgaagttgac cgaggccgtg gaactgagcg tccagcgggg catgttgggc aactggccgc
     1921 ccggctgcac gatggtgctg tcctcggacc gcgtgacatg gccgttgttg tacccggcgt
     1981 tacccagcag ggtgacgctt ctgaaaggca gccaggccag cgaggcttcg gctccgtaca
     2041 ccaccgcatc gccggcgttg tctaggtgcg cgaagttcgg aaccgggacc aggcccagct
     2101 gcgccgtacc cagcacggtg ccctgcatgt ccttccagtc gatgtggtac gcggcgatgt
     2161 tgctggtcag gcggccatcg agccaggtgg acttgctgcc gatctccatg ttcagcacgt
     2221 agtccggccg gatgatcggc ggacccgagt gcagcgcgga atagatgttg gaggcaccgg
     2281 agcggaagcc gcggctgatg gtcaggtagg ccatgtggtc ctggttgaaa tgccaggcgg
     2341 ccgacagctt gggcaggaag ccactgctct tcatgcgctg ctggatgtcc acgcgcgcgg
     2401 tcttggtctg cggatcgatc agggtcgggt ccgacccgat caggtacgcc tcaaggccat
     2461 acaggtgggt gtcgaagaag aagttgatct cttcgctgaa ataacgcagc ccgccggtga
     2521 tgtcgagcgt gtcgggaatc gcattccatt tcagttcacc gtaggcggct tcctgggtga
     2581 aggcttcatc gccggtaccg tgctcctgct ccccttgcac cgggtaaaag ccgccgagga
     2641 ggccggtgac caggccgccg gtcggatcga tggtgggcac cgtgtcctcg cgcttcgcct
     2701 cgtcgaatga ctggtgacgg tcgcggtaga aggcgccggc gatccagtcg aagcgctcgc
     2761 cgccgaacga caccaggcgc agttcctgcg agaacgaatg caggccggta acgacgcgtg
     2821 tcgaggcgtt gctgaagatg tccggcgccg gtccgaggcc gagcgtcagg aactgcccgt
     2881 tgatcagcgt ctgcagcacc ggggtgcggc ggcgggtgtc cttgtccgtg tcatacagcg
     2941 cactgaccga ggtgaggttg gcgaacggca gggtccagcg cagcgtcagc gcgtcgatgg
     3001 cgaagcgggt cttggcgtac tggtcctcga cgcgcgagtt ggtcagctcc tcctgcttgc
     3061 cctcgtccac caccggaaat tgttccttgt ggctgaaatc ctgcaggtgc agcaggtcgg
     3121 cctggaggtt ttcggaaacc tgccagcgca gcaggccccg caccgagtcg gcgcgttcct
     3181 ggtcggcgtc gggccggttg aggttcacgt agtcaacgaa gccgccagtg acgcgcgcgg
     3241 tccccacgac gcgcagccct acgcggtcgt gccatagcgg cgcattgacc gcgaggcggg
     3301 tgtcgtagct gggcgagcct tgctcggtga tggcgtaact gctgttgccg cgcagttccc
     3361 attcgttgaa cgagggctca cgcgtgatca tcttgatggc gccgcccatg gaaccttcgc
     3421 cgtaaagcgt gccttgcggg cctttcagca cctcgatgcg cgccaggtcg tagatgttga
     3481 gatcgggaaa cacgccggaa ccctggatgg cgacgtcgtc gaggtacacg ccggtggtcg
     3541 gcgaaccgtc ggcatagccg agatcggagg tgttcacgtt ggagatgccg cgcatggcga
     3601 tcttggcggt gccgttgccg gatttctgca gcgacacgct gggcacctgc accaggtaat
     3661 cctgcatgcc ggtggcgccg gccttgtcca gcgcgcgtcc accgaatgcg tgtgcgctgc
     3721 cggcgacgtc ctggatgttt tccttgcgat atgacgcggt gacctccacg gtatccagcc
     3781 gctgcgtgcc gtcatcggcg gcggcgttcg cggattccgg gtgcaggtcc tgcacggcga
     3841 tggtggccgg cgcgggcgcg ggttctgccg gcgtggcgct gctttcggca gcagttccgg
     3901 cgaccggctg gtcggcggga atcgaagcgg gagtggcctc tgccggggcg gtgtcggcgg
     3961 ccggcgcgga cggccctggc gcggacgacg gcacggcgcc ggtgccatcg tcctgggcct
     4021 gcgccactcc tgccgacacc agcagcagaa cggcggcagt catcccttgc agcatccgat
     4081 tcattgtctc tcctcgccac gcgccttcga tatgagacgt ttatgaatac atcatgtatt
     4141 gaaatataaa acgtgtaaga tgagcttgca aatgggcaag accaaccaat ccgccgtccg
     4201 tccggccgcg ccgcgcgcgc agccggcgcg cacgcgcctc acgcgcgccg aggccaaggc
     4261 ccagcgcacg cagcaattgc tcgatgcggc ctgggagctg ttctgcgagc gcggctatga
     4321 agcgctgacc atcgatcacg tggccgagcg cgccggctac tcgcgcatgc cgatctactc
     4381 gctgttcggc gacaagcaga acctgttctt cgagctgtgg cgcgccaaga cggcgcagct
     4441 tacccaggtg ctgatcggcg gcttcgcgcc gggcgcatcg ctgcgcagga acatccggca
     4501 gctggccgag gtgctcgctt cccggccgat ggtcgaagcg ccgatgcacg cggagagcat
     4561 gttcttcgtg gtgcagacca tcgcactgtc gcggcctgat ctcgaggaga agctgcaggc
     4621 gctggcgcgc aaggtgatcg acgactttgc cgggatgatc cgccgcgccg cgctggaaaa
     4681 aggcgacgtg ctgcgcggca cgcccgaggt ggtggcggcg cacatggtgg cgcacatcaa
     4741 cggcatggcc aatgtgaagt tccagaccgg cggccagtac accaaagcac gcgacctggt
     4801 cgccatcttc aacgccatcg ccttcaagtc gccggatgag tgatgtgccg cgcctgtcgg
     4861 ccgccacgct ggcggcggcg gctcctgacg tggaaccccc gcgctacgcg cgccgcagcg
     4921 agcggcgcat ttgccatatc ggtgccggca ggttccaccg cgcgcaccag gcgtactacc
     4981 tgcaccggtt gttgcagcag ggcgacggtg aaggctgggg catctgcgcc atcggcctga
     5041 agcgggccga tgccgaagtg cacgcacgcc tgcagccgca ggatcagctg tattcgctgt
     5101 gggaactcga cggcgacaag cggcgcggca ccgtcgtcgg cagcctgatg caatgggtgg
     5161 acgccgcggc ggatgccgat gccgccatcg cggtgatggc cgacccggcc acgcacatca
     5221 tctcgctcac ggtgaccgag tctggttact gcctcgatgc gcgcggcgcg ctggattgcg
     5281 cccacccgga tatcgcgcat gacctggcgg gcggtgcccc gcgcagtgcg ccgggactga
     5341 tcgtgcgcgc gctggccgcg cgccgcgccg ccggcggcag cggagtgacg ctgatgtcct
     5401 gcgacaacct gctggagaac ggcaaggtgc tgcgcgcctg catgcttcac tttgcgcagc
     5461 gcgtcgacgc ggcgctggag cggtggatcg agcgcgagtg cagcttcccc tgctcgatgg
     5521 tcgaccgcat cacgccggcg gcggacgacg cgcgcagcgc gcagctttcg cgcgaactcg
     5581 gcctgcgtga cgacgcgctg gtgttctgcg agccgtggca gcagtgggtg atcgaggatc
     5641 actttgtcgc cggccgtcct gcgttcgaga aagccggcgc ggtgttctcc atgcgcgtac
     5701 gcgactacga gcgcatgaag gtcggcatgc tcaacggcgg ccattccgcg ctgtcgcacg
     5761 tgggtctgct gctggggcat acgcgcgtgc acgaagcact ggccgtgccc gagctgcgtc
     5821 tctggctcgc ggcctacatg gacgacgttg cacaggtcct gccgccgctg cccggcgtca
     5881 attacctgga atatcgcgat gcattgctcg cgcgcttttc caacccggcc atcgaggatc
     5941 gcctggcgcg gctggcgctg gacaccacgg ccaagtacc
//
LOCUS       DASZZC010000807         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_893370,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000807 DASZZC010000000
VERSION     DASZZC010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893370"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="VHE37_17445"
     CDS             complement(<1..1897)
                     /locus_tag="VHE37_17445"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HVT37375.1"
                     /translation="MPFKSAAFLLTLASTMLSAAALAASPPAAVDGARIQAADSEPSN
                     WMSHGRTYTEQRYSPLAKINDGNVDQLGLAWTYKLDVDRGVEATPVVIDGTMYTTGAY
                     SIVYALDARTGKLKWKFDPKVPRARANDGCCDVVNRGVAAWKGKIYVGSFDARLIALD
                     AATGKKVWESDTSIDHSRSYTITGAPRVVKDKVIIGNGGAEFGVRGYISAYDAATGKL
                     VWRFFTVPGDPSKPPENKAMEMAQKTWYGDQYWKFGGGGTAWDSMAYDPELDLLYVGT
                     GNGSSWNRKVRSEGKGDNLFLSSILALRPDTGEYVWHYQTTPGDTWDYTATQSIILAD
                     LKIDGQTRKVLMQAPKNAFFYVLDRATGQLLSADKFAPSNWATEVDMKSGRPIENPEA
                     DWTLQPRLVFPSPFGAHNWQPMSFNPKTGLVYIPSQETMIQLAPDNKAKYVRRGVFNV
                     GVQPFDMPEGMKGMEPIGQAYKGHLLAWDPVAKKPAWTVDYPAIWNGGTLTTAGNLVF
                     QGTADGRAVAYAADSGKKLWEAPANTGVMAGPVTFTARGEQYVTFMAGWGGAYGLAGG
                     AVIAAQGGKTRAEARVITFKLGGTGKLPPPKNAPAPLPEPPPVTADEATLGLGRALYN
                     GYCGVCHG"
BASE COUNT          352 a          737 c          762 g          436 t
ORIGIN      
        1 gcccgtggca cacgccgcag tagccgttgt acagcgcgcg gccgaggccg agcgtggctt
       61 cgtcggcggt gaccggcggc ggttccggca gcggcgccgg cgcgttcttc ggcggcggca
      121 gtttgccggt gccgccgagc ttgaaggtga tgacgcgggc ttcggcgcgc gtcttgccgc
      181 cctgcgccgc aatcaccgca ccgccggcca gtccatacgc gccgccccag ccggccatga
      241 aggtcacgta ctgctcgccg cgcgcggtga acgtcaccgg gccggccatc acgccggtgt
      301 tggccggtgc ctcccacaac ttcttgccgc tgtctgcggc ataggccacg gcgcggccgt
      361 cggcggtgcc ctggaagacg agattgcccg cggtggtgag cgtgccgccg ttccagatgg
      421 cgggatagtc gacggtccac gcgggcttct tcgccaccgg gtcccacgcc agcagatgac
      481 ctttgtacgc ctggccgatc ggctccatgc ccttcatgcc ctcaggcatg tcgaacggct
      541 gcaccccgac gttgaacacg ccgcgccgca cgtacttcgc cttgttgtcg ggcgccagct
      601 ggatcatggt ttcctgcgac gggatgtaga ccagcccggt cttcgggttg aacgacatgg
      661 gctgccagtt gtgcgcgccg aacggcgagg ggaacaccag ccgcggctgc agcgtccagt
      721 cggcctcggg gttctcgatc ggccggccgc tcttcatgtc gacctcggtg gcccagttcg
      781 acggcgcgaa cttgtcggcc gacagcagct ggccggtggc gcggtccagc acgtagaaga
      841 acgcgttctt cggcgcctgc atcagcactt tgcgcgtctg cccgtcgatc ttgaggtcgg
      901 ccaggatgat gctctgcgtc gccgtgtagt cccaggtgtc gcccggcgtg gtctggtaat
      961 gccacacgta ttcgccggtg tcgggccgca gcgcgaggat ggaagagagg aacaggttgt
     1021 cgcccttgcc ttcgctgcgc accttgcggt tccacgacga gccgttgccg gtgcccacat
     1081 acagaagatc aagttcggga tcgtaggcca tcgaatccca ggcggtgccg ccgccgccga
     1141 acttccagta ctggtcgccg taccaggtct tctgcgccat ctccatcgcc ttgttctcgg
     1201 gcggcttcga cggatcaccc ggcacggtga agaagcgcca caccagtttt ccggtcgcgg
     1261 catcgtaggc ggagatgtag ccgcgcacgc caaattcggc gccgccgttg ccgatgatca
     1321 ccttgtcctt cacgacgcgc ggcgcgccgg tgatggtgta gctgcgcgag tgatcgatgc
     1381 tggtgtcgct ctcccacact ttcttgccgg tggcggcgtc cagcgcgatc agccgcgcgt
     1441 cgaacgagcc gacatagatt tttcctttcc acgccgccac gccgcggttg accacgtcgc
     1501 agcagccgtc gttggcgcgc gctcgcggca ctttcggatc gaacttccac ttgagcttgc
     1561 cggtgcgcgc atcgagcgca tagacgatgg aataggcgcc ggtggtgtac atggtgccgt
     1621 caatcaccac cggtgtggct tccacgccgc ggtcgacgtc gagcttgtag gtccaggcca
     1681 gcccgagctg atcgacgttg ccgtcgttga tcttggccag cgggctgtag cgctgctcgg
     1741 tgtaggtgcg gccgtggctc atccagttgc tgggctcgga gtccgcggcc tggatgcgcg
     1801 cgccgtcgac tgcggcgggc ggacttgccg ccagtgctgc cgcggagagc atggtgctgg
     1861 caagcgtgag caaaaaagct gcggacttga acggcatggg gattctcctc gttgtcatcg
     1921 ccggacttca gctgcgcgcg cgtactgtac tccagcggcg cggtgcgccc gctactcgcc
     1981 cgggtggggc gtgccgcgct ttttgggctg taccgcaccg gcaatctggc tgttataaaa
     2041 aatcatttca tatttaaaat aatcaataat aaataacatc agaaatgggc atgcggtgga
     2101 gataaaaatg accgcctgcg agaggtggcc gggacgccgc cgctacaatc gcgattgggg
     2161 agaacatgtt cacacatcag gaattgcgcg cggccagcgc ggtggtctac gcgcacatgc
     2221 cgccaacgcc gcagcgcgag tggccattgc tttcgcatcg gctgtgcgca caggtgtggg
     2281 tgaagca
//
LOCUS       DASZZC010000808         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_893531,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000808 DASZZC010000000
VERSION     DASZZC010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893531"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VHE37_17450"
     CDS             <1..314
                     /locus_tag="VHE37_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HVT37376.1"
                     /translation="LNMPTANIALHRAPALRLGVYAVRARLQGRVHDGVASLGVRPTL
                     GGTPCLFETHLFGTHGALYGQVMEVEPVMFLRGEEKFDSLDALAGQMQRDKARAMEIL
                     R"
     gene            611..3466
                     /gene="ileS"
                     /locus_tag="VHE37_17455"
     CDS             611..3466
                     /gene="ileS"
                     /locus_tag="VHE37_17455"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HVT37377.1"
                     /translation="MDTKRDYKSTLNLPETRFPMQANLAKREPEALARWEAEGLYACV
                     QQLSAGKPPFWFVDGPPYANGDIHIGHAVNKTLKDIVVKAARLDGYDAPFVPGWDCHG
                     LPIEHQVEKKHGKVGDKLDAKTFRAKCREYAGEQVKRQRVDFKRLGVLADWERPYLTM
                     NPQFEAEQLRVLARIVDNGHVVRGFKPVHWCLDCGSSLAEAEVEYADKESFAIDVKFA
                     AADAADLARRFGVSDAGGASFVIWTTTPWTLPANQAIAVNAELEYALVELENSGRVVL
                     ASEMVEGVAKRYGESARVLARAKGAALEGARAQHPFAPRTVPVILGEHVTLDAGTGLV
                     HTAPAHGVEDYVVGQQYKLPVDNPVASNGKFVAGTPIVEGVFVRKAEEPIIEALRERG
                     ALAHLAKITHSYPHCWRHKTPLIFRATPQWFVSMEQAGLRVQALAAIRRTNWIPEWGE
                     ARIHEMIATRPDWCISRQRYWGVPMAVFIDRAKQQLHPQTSELLKRIAEKVEREGLEA
                     WFGSQPSDWGVDEAAYEKCTDTMDVWFDSGSVSQCGFTSTGHPVPDYAALDSADNRDG
                     AKNGAKRKVFYLEGSDQHRGWFHSSLLVRTAVDTHPPYTHVLTHGFTVDEQGRKMSKS
                     LGNVIEPQKVTGTLGADVLRLWVAASDYSGEIAISEQLLQRMADAYRRIRNTARYLLG
                     NLNGFDPAKDMVAPAEMVAVDRWAVAQAAALQEEIKAAYRASQFHLVYHKLHNYCVND
                     LGGLYLDILKDRLYTLPAKSMGRRSAQTALLHIAEAMVRWIAPVLSFTADEIWRELPG
                     DRSAPVFAQEWHAFPPVGAENIDWAALSDVREHVKKVLESLRIKGAIGSGLNAQVQLH
                     AEGKTGATLQQFGDELRFWFITSGAQVLPSKPADATEAALPGGEKVWVSAAASPHEKC
                     ERCWHQLPDVGRHADHPTLCGRCVLNVGGQGEVRKYV"
BASE COUNT          633 a         1107 c         1245 g          582 t
ORIGIN      
        1 agctcaacat gccgacggcg aacatcgcgc tgcaccgcgc gcccgcgctg cgcctgggtg
       61 tgtacgcggt gcgcgcgcgc ctgcaggggc gcgtgcacga cggcgtcgcc agcctcggcg
      121 tgcggcccac gctcggcggc acgccatgcc tgttcgagac gcacctgttc ggcacgcacg
      181 gcgcgctgta cgggcaggtg atggaagtcg agccggtgat gttcctgcgc ggcgaagaga
      241 agttcgattc gctcgatgcg ctggcggggc agatgcagcg cgacaaggcg cgtgcgatgg
      301 agattctgcg ctgaggtgcg ccgtccacat cccgtcggag cgcgccttgc gcgcgatgga
      361 tctgtttgcg gagcggagaa tcgcgcgcaa ggcgcgctcg tacaaaagcg tcgatgccat
      421 gcacccgcga cgggcttgcg gtttataaag ggtcctgcag ccctcataat gtgccgaccg
      481 caagtggctt tcgcctgtta cgttttatta tctgatttat aaatcgttaa atattttata
      541 acgcacattt ggccggtgcg gttgacctgc tgacggcgcc tttttttgaa gcgacattct
      601 gaagcgatac gtggatacga aacgagacta caagagcacg ctgaacctgc ctgagaccag
      661 gttccccatg caggcgaacc tggccaagcg cgagccggaa gcgctcgcgc gctgggaagc
      721 ggaaggcctg tacgcgtgcg tgcagcagtt gtccgcaggc aagccgccgt tctggttcgt
      781 cgatgggccg ccgtacgcca acggcgacat ccacatcggc cacgcggtga acaagacgct
      841 caaggacatc gtggtgaagg cggcgcggct cgacggctac gacgcgccgt tcgtgccggg
      901 ctgggattgc cacggcctgc cgatcgagca ccaggtcgaa aagaagcacg gcaaggtggg
      961 cgacaagctg gacgcaaaaa ccttccgcgc gaaatgccgc gaatacgccg gcgagcaggt
     1021 caagcgccag cgcgtggact tcaaacgcct gggcgtgctc gccgattggg agcgacccta
     1081 cctgacgatg aatccgcagt tcgaggcgga gcagctgcgc gtgctggcgc gcatcgtcga
     1141 caacggccac gtggtgcgcg gcttcaagcc ggtgcactgg tgcctggact gcgggtcgtc
     1201 gctggcggaa gcggaagtcg aatacgcgga caaggaatcg ttcgccatcg acgtcaagtt
     1261 cgccgcggcc gatgccgccg atctggcgcg ccgcttcggc gtgagcgatg cgggcggcgc
     1321 ctcgttcgtc atctggacca ccacgccgtg gacgctgccg gccaaccagg ccattgcggt
     1381 caatgccgag ctcgagtacg cgctggtcga actggagaat tccggccgcg tggtgctggc
     1441 ttccgagatg gtcgaaggcg tggccaagcg ctacggcgag tccgcgcgtg tgctggcacg
     1501 cgcaaagggc gcggcattgg aaggcgcgcg cgcgcagcac ccgttcgcgc cgcgcacggt
     1561 gccggtgatt ctcggcgagc acgtcacgct cgatgccggc accggcctgg tccacaccgc
     1621 gccggcgcat ggcgtggagg actacgtggt cggccagcag tacaagctgc cggtcgacaa
     1681 cccggtggcg agcaacggca agttcgtggc cggcacgccg atcgtcgaag gcgtgttcgt
     1741 gcgcaaggcc gaggagccga tcatcgaggc gctgcgcgag cgcggcgcgc tggcgcacct
     1801 ggcgaagatc acgcattcgt atccgcattg ctggcgccac aagacgccgc tgatcttccg
     1861 cgctacgccg cagtggttcg tctcgatgga gcaggccggc ctgcgcgtgc aggcgctggc
     1921 cgccatccgc cgcaccaact ggatcccgga atggggcgag gcgcgcatcc atgaaatgat
     1981 tgcaacgcgc ccggactggt gcatctcgcg ccagcgctac tggggcgtgc cgatggcggt
     2041 attcatcgac cgcgcgaagc agcaactgca tccgcagacc tcggaactgc tcaagcgcat
     2101 cgccgaaaag gtcgagcgcg aaggactgga agcctggttc ggctcgcagc cgtcggactg
     2161 gggagtggac gaagctgcct acgagaagtg cacggacacc atggacgtgt ggttcgactc
     2221 cggctcggtc agccagtgtg gcttcacgtc caccggtcac ccggtgcccg attacgccgc
     2281 gctcgacagc gcggacaacc gcgacggcgc caagaacggc gcgaagcgca aggtgtttta
     2341 tctcgaaggc tcggaccagc accgcggctg gttccattcc tcgctgctgg tgcgcacggc
     2401 ggtggacacg cacccgccgt atacgcacgt gctcacgcat ggcttcaccg tggacgagca
     2461 gggacgcaag atgtccaagt cgctcggcaa cgtgatcgag ccgcagaagg tgaccggcac
     2521 gctgggcgcc gatgtgctgc gcctgtgggt ggcggcgagc gattactcgg gcgagatcgc
     2581 catctccgag cagctgctgc agcgcatggc cgatgcgtat cgccgcatcc gcaacacggc
     2641 gcgctacctg ctgggaaacc tcaatggctt cgatccggcg aaggacatgg tcgcgccggc
     2701 ggagatggtg gcagtagacc gctgggcagt cgcgcaggcg gcggcgctgc aggaagagat
     2761 caaggccgcg taccgcgcca gccagttcca ccttgtctat cacaagctgc acaactactg
     2821 cgtcaacgac ctgggcgggc tgtacctcga catcctcaag gatcgcctct acacgctgcc
     2881 ggcgaagagc atgggtcgcc gctcggcgca gaccgcgctg ctgcatatcg cggaagcgat
     2941 ggtgcgctgg atcgccccgg tgctgtcgtt caccgccgac gagatctggc gcgagctgcc
     3001 gggcgaccgc agcgcgccgg tgtttgcgca ggagtggcac gcgtttccgc cggtcggtgc
     3061 ggagaacatc gactgggccg cgttgtctga cgtgcgcgag cacgtgaaga aggtgctgga
     3121 gtcgctgcgc atcaagggcg cgatcggttc cggcctcaat gcgcaggtgc agttgcatgc
     3181 cgagggcaag accggtgcga cgctgcagca gtttggtgac gagctgcgct tctggttcat
     3241 cacctccggc gcgcaggtgc tgccttcgaa accggcagac gcgacggaag ctgcgttgcc
     3301 aggcggcgag aaggtctggg tcagcgccgc agcgtcaccg cacgagaagt gcgagcgctg
     3361 ctggcaccag ctgccggatg taggcaggca tgcggatcat cccacgctgt gcggccgctg
     3421 cgtgctgaac gttggcgggc agggcgaagt gcggaagtac gtttaaggag cgcgcgtgtc
     3481 ggttcaatac aggaacgtct actggctgtg gctctcggcg ctggtcatct tcctcgacca
     3541 ggtgaccaag cagctggtgg tgaagca
//
LOCUS       DASZZC010000809         6290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_768596,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000809 DASZZC010000000
VERSION     DASZZC010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768596"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6290
                     /locus_tag="VHE37_17460"
     CDS             <1..>6290
                     /locus_tag="VHE37_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT37378.1"
                     /translation="GDRNARDKVYSRWGGFLEEVPFDPTSYGIPPRSMKSIDPMQLLA
                     LEVARRTLADAGYAQGGFDRENTSIILGAGGGLGDLGLQYGVRAELPRFVENPDPGVW
                     ERLPEWTEESFAGTLLNVAAGRVANRMDFGGLNFTIDAACASSLAAINVAVSELESGR
                     SSMVLAGGIDTVQSPFGYLCFSKTQALSPTGRPKTFDESADGIAISEGLAVVALKRLA
                     DAEADGDRIYAVIKAVAGSSDGKALGLTAPRPDGQIRALKRAYAKAGFSPSTLGLFEA
                     HGTGTAVGDRAEAETITRALKNEGAAPRSVALGSVKTILGHTKASAGVSGLIKVALSL
                     YHRVQPGHHGVEKPIAAISDPASPVALFKDARPWIAHPRHPRRAGVSAFGFGGTNFHA
                     VLEEYAGAHESPGADRWPHELCVFRAENDAALIKDLEQVLPGLVPGSRVALASIGATL
                     ARRAKSRGAQPVTLALVAADHEALKKDITAVLSHLRESKPLPPHIRLNRTTPAQAPET
                     AFLFPGQGAQYTGMGREAALYFDDVRTAVELADRQLADRFPDLLSRTILPASPFDADI
                     EKRQTAELTRTSVAQPAIGAVSLGYLRLARRLGLSATATAGHSYGEYGALCAAGVIGE
                     ADLLELSAVRGAAMEQAAQTSLPGTMAAVQGAREAIAAALDDFPGVRVANHNAPQQSV
                     ISGPREAVVAACTKLEGLGMRATPLPVSGAFHTDLVAAAQEPLAKAIRACRFHPPAAT
                     VYSNSAGKAHAREPAEIRAAMENHLLSSVEFVAEIEAMYAAGCRVFVELGPKSLTSNM
                     VKQILSGRDHVAVSLDGNGGGLRGLLQGLAELFVAGTRLDAMQLFAARDVAGIEISQL
                     GALALPQPLPSHVWMVSGGAARPLSDPIRRTGREPALTRAAVDAARAKVAAAQAASPS
                     PGMVAPATAQPIPAAPAPAMVPVPATMGTEALVAYQQTMRQFLALQEKVMQQMLGGMP
                     MPAAPAPALAPLAAPAPPPPAPIQVATIVTPAAAPAKAPIAVVQDFDAPAILLEIVCQ
                     RTGYPAEMLALDADLEADLGVDSIKRVEILGALRKALPSAQATAMQAAMEQFTKARSI
                     SAILRELVKLGGATTIAAPAPTAVAPAAVDHTKLLIGIVADRTGYPADMLALDADLEA
                     DLGVDSIKRVEIIGALRKAVSTVTAAAMQDAMEKFTKARSLKSILAELDKLAVPATVA
                     DAPLAPAAPAAAAAPIDYMKLLTGIVAERTGYPAEMLTADADLEADLGVDSIKRVEIL
                     GALRKALPAAQSAAMQASMEKYTKARSLKAIVAECLQLAPATAPAPAVAAATATATAA
                     ADLNLRSLLTGIVAERTGYPAEMLSAGADLEADLGIDSIKRVEILGALRKALPAGMAE
                     QMRADMERYTKARTLDAILAQLSAVERTPLVAASATLAVDTAAPAAPAVLQKIPRFVI
                     KPQPAPLAETAVQLQGLALVFGGPRAIVDLVQLQLEACGLTPVGIDAVDGEIIAGQIA
                     QAQQKHGPARAVLHLHGLTSASPQSLGEWRNLGQHAVYSLFHAARALDQGLNNCRVIV
                     GTRLGGTFGRDAVGSGAPLAGGACGITNCLRYEYPSAHFRAVDFDGQSDAEIAQLLVA
                     ELHSEDRASEAGYIGSERYGSITELQPLTQNPFAHNVKPQKDWVLLVTGGARGITAEI
                     IDELACDGLRLVLLGRTAEPAAEALATRPLKSTTELRQHLTQQALGRGEKPKPVDIDR
                     AVSALLAEREIRDNLARLRAKGCIVDYIACDVRDERSLGQVIDDVYAKHGRLDGVIHG
                     AGVIEDRLIRDKTEASFARVFGTKLDPVFVLQSRLKADSLKFLALFASVAGRYGNRGQ
                     SDYAAANEALNRLAWSLSRAWPQTRVMAINWGPWDAGMASDGVKKQFRERGIEPIAVA
                     AGRRFFVEELEFGSRNHVELVAGEGPWGAVTSEPPAAVPAALSVPLVRGDLRLGPGGS
                     MTLDHTYSLGSDPYLADHKLDGKPVLPATGALEWIAQFAAAAWPTWQVSEIRDLRVLN
                     GIVLDGGGDRNVQLRARASTHSDPGEQAITVEIVDLVRKLPCYRATVILAEQLAMAAA
                     QTPSAIGGTPFDPARAYREYLF"
BASE COUNT         1071 a         2144 c         2067 g         1008 t
ORIGIN      
        1 ggcgaccgca atgcgcgcga caaggtctat tcgcgctggg gcggcttcct ggaagaagtg
       61 ccgttcgatc cgaccagtta cggaatccct ccgcgctcga tgaagtcgat cgatcccatg
      121 cagctgctgg cgctggaagt cgcgcggcgc acgctggcgg acgccggcta cgcgcagggc
      181 gggttcgatc gcgaaaacac ctcgatcatc ctcggggccg gcggcggact gggcgatcta
      241 ggcctgcagt acggcgtgcg tgcagaattg ccgcgcttcg tcgaaaaccc cgatccgggc
      301 gtttgggagc ggctgccgga atggaccgag gaatcgttcg ccggcacctt gctcaacgtc
      361 gccgcggggc gggtcgccaa ccgcatggat ttcggcggcc tcaatttcac catcgacgcg
      421 gcgtgcgcct cgtcgcttgc cgccatcaat gtcgccgtca gcgaactgga gagcggacgc
      481 agctccatgg tgctggccgg aggcatcgac acggtgcaat cgccgttcgg ctatctgtgc
      541 ttctcgaaaa cgcaggcgct gtcgccgacc ggcaggccca agaccttcga cgagtcagcc
      601 gatggcattg ccatcagcga aggcctggcc gtcgttgcgc tcaagcgcct ggccgatgcc
      661 gaagccgatg gtgatcgcat ctatgccgtg atcaaggcgg tagccggttc gtccgacggc
      721 aaggcgctgg ggctgaccgc accgcggccc gacggccaga tccgcgcact caagcgcgcg
      781 tacgccaaag cagggttttc gccgtccacg ctggggctgt tcgaagcgca tggaacaggc
      841 actgccgtcg gcgatcgcgc cgaagctgaa accatcacgc gtgccttgaa gaacgaaggt
      901 gcagcgccgc gcagcgtcgc actcggttca gtcaagacca tcctcggaca caccaaggcc
      961 agtgcgggcg tgtctggcct gatcaaggtc gctctttcgc tttaccaccg ggtacaaccc
     1021 ggacatcacg gcgtggagaa acccatcgcc gcgatttccg acccggcatc gcctgtcgcg
     1081 ctgttcaagg atgcgcgccc gtggatcgca catccgcgcc atccacgccg cgccggcgta
     1141 tcggcgttcg gcttcggcgg caccaatttc catgcggtac ttgaggaata tgccggcgcg
     1201 cacgaatcgc cgggcgcgga tcgctggccg cacgagctct gcgtcttccg cgccgagaac
     1261 gatgcagctc tgatcaagga tctggagcag gtgctgcccg gactggtccc cggcagccgc
     1321 gttgccctgg catcgatcgg tgccaccctg gcgcgccgtg caaaatcgcg tggagcgcag
     1381 ccggtgacac tggcactggt cgccgcagat cacgaggcat tgaagaagga catcacggcg
     1441 gtgctgtcgc acctgcgcga atcgaaaccg ctgccgccgc atatccgcct gaatcggacc
     1501 actccggcac aagcgcccga aaccgccttc ctgttcccgg gccagggtgc ccaatacacc
     1561 ggcatgggcc gcgaggccgc gttgtacttc gacgacgtac gcacggctgt cgaactggcc
     1621 gatcgccagc tggcggatcg ctttccggat ttgctctcgc gtacgatcct gccggcctca
     1681 ccattcgatg ccgatattga aaagaggcaa acggccgagt tgacgcgcac cagcgtcgcg
     1741 caaccggcga tcggcgccgt ctcgctgggc tatctgcggc ttgcgcgcag gctgggcctg
     1801 tcggccaccg ccactgctgg tcacagctac ggtgaatacg gggcgctgtg cgctgccggc
     1861 gtgatcggtg aagcggacct gctcgaactc tctgcggtcc gcggcgctgc gatggaacag
     1921 gccgcacaga catccctgcc cggaaccatg gcagccgttc agggcgcgag ggaagcgatt
     1981 gccgccgccc ttgacgattt ccccggtgtg cgcgtggcca accacaatgc tccgcagcaa
     2041 agcgtgattt cggggccgcg tgaagccgtg gtcgccgcgt gcaccaagct ggaaggcctc
     2101 ggcatgcgcg caactccgct gccggtttca ggcgccttcc acacggacct tgtcgccgcc
     2161 gcgcaggaac cgctggccaa ggcgatccgt gcatgccggt tccatccgcc ggcagccacg
     2221 gtttactcga acagcgccgg caaggcgcat gcgcgggagc ccgcagaaat tcgcgcggcc
     2281 atggaaaacc acctgctctc gagcgtggaa ttcgtcgcgg agatcgaggc gatgtacgcg
     2341 gccggctgcc gtgtattcgt ggagctggga cccaagagcc tgaccagcaa catggtgaag
     2401 cagatccttt ccgggcgcga tcatgtcgcc gtctcgttgg atggcaacgg cggaggcctg
     2461 cgcggactgc tgcaaggtct tgcggaactg ttcgttgcag gcacccggct tgatgcgatg
     2521 cagctcttcg cagcccgcga tgtcgccggc atcgagattt cccagctcgg cgctcttgcc
     2581 ctgccacagc cgctgccatc gcacgtctgg atggtcagcg gcggcgcagc ccgtccgctg
     2641 tccgatccga tccgccgcac gggccgcgag ccagccctga ccagagccgc ggtcgatgcc
     2701 gcgcgtgcca aggtagcggc cgcgcaggcg gcatcgccat cgccgggcat ggtggcccct
     2761 gcaactgcgc agccgatccc ggccgcccca gcacccgcga tggtaccggt gccagcaacg
     2821 atggggacag aagccctggt cgcgtatcag cagaccatgc gtcagttcct ggcgctgcag
     2881 gaaaaggtga tgcagcaaat gctcggcggc atgccgatgc cggccgcgcc ggccccggcc
     2941 cttgctccgc tcgcggctcc tgcgccgcca ccaccggcgc ccatccaggt tgcgacgatt
     3001 gtgacgccgg cagccgcacc ggcgaaagct cccatagcgg tcgtacagga tttcgatgca
     3061 ccagcgatcc tgctcgaaat cgtatgccag cgcaccggct atccggccga aatgctcgcg
     3121 ctggatgctg atctcgaagc cgatctggga gtggattcga tcaagcgtgt cgagattcta
     3181 ggcgcgctgc gcaaggcctt gccctcagcg caggcgaccg ccatgcaagc tgccatggaa
     3241 cagttcacca aggcgcgttc gatcagcgcg atccttcggg agttggtaaa gcttggcggt
     3301 gccaccacca tagccgcgcc agcgccgaca gccgttgctc ctgctgcggt ggatcacacc
     3361 aagctgctca tcggcattgt tgcggaccgc acgggctatc ccgccgacat gctggccttg
     3421 gacgccgatc ttgaagccga cctcggcgtg gattcgatca agcgcgtcga aatcatcggc
     3481 gccctgcgca aggccgtgtc caccgttact gcagcggcta tgcaggacgc gatggaaaaa
     3541 ttcaccaagg cgcggagcct gaaatccatc ctggcggaac tggacaagct cgcagtccct
     3601 gccaccgttg ccgatgcgcc attggcgccg gcagcacccg ccgctgctgc ggcgcccatc
     3661 gactacatga agctgctcac cggcatcgtc gccgagcgca caggttaccc ggccgagatg
     3721 ctgacggccg acgcggacct tgaagccgac ctcggcgtcg attcgatcaa gcgcgtggaa
     3781 atccttggcg cgctgcgcaa ggccttgccc gctgcgcagt cggcggcgat gcaggcgtcg
     3841 atggagaaat acaccaaggc acgcagcctc aaggccatcg tggccgagtg cctgcagctt
     3901 gccccggcca cggcgcctgc acccgccgtt gcagcggcga ccgccactgc aacggcagcc
     3961 gccgatctga acctgcgctc gctgctgacc ggcattgtcg ccgaacgcac gggatacccc
     4021 gccgaaatgc tcagtgcagg cgctgatctg gaagcggatc tgggcatcga ctcgatcaag
     4081 cgcgtggaaa tcctgggcgc actgcgcaaa gccttgccgg ctggcatggc cgagcagatg
     4141 cgcgcagaca tggagcgcta taccaaggcg cgcacgctcg atgcgatcct ggcgcagctt
     4201 tcggcggtag agcgcacgcc gctggttgcc gcctccgcga cattggcggt ggatactgcg
     4261 gcgcccgccg ctcccgccgt gttgcagaag attccgcggt tcgtgatcaa gccacagccg
     4321 gcaccgcttg ctgagaccgc agttcagttg caggggttgg cgctggtgtt tggtggcccg
     4381 cgtgccatcg tcgatctggt ccagctgcag ctggaggcct gcggactgac cccggtaggc
     4441 atcgacgccg tcgacggcga aatcatcgct ggccagatcg cccaagccca gcagaagcat
     4501 ggtccggccc gggccgtact gcatctgcac ggcctgacga gcgcatcccc gcaatccctg
     4561 ggcgagtggc ggaacctcgg ccagcacgcg gtgtacagcc tgttccacgc agcacgcgct
     4621 ctcgaccagg ggctcaacaa ctgccgtgtc atcgtcggca cgcggctggg cggtactttc
     4681 ggccgcgatg cggtgggctc cggcgcgccg ctggccggcg gcgcgtgcgg catcaccaac
     4741 tgcctgcgct acgaatatcc gtccgctcat ttccgcgcgg tggatttcga cgggcagagc
     4801 gatgcggaga tcgcgcagct gctggtggcg gaactgcaca gcgaggaccg cgctagtgaa
     4861 gccggttaca tcggcagcga acgctacggc agcatcaccg agttgcaacc cctcacccag
     4921 aatccctttg cgcacaacgt gaagccgcag aaggattggg tgctgttggt gaccggagga
     4981 gcgcgcggca tcaccgccga aatcatcgat gagctggcgt gcgacggcct gcgcctggtg
     5041 ctgctgggac gcaccgcgga accagccgcg gaagctttgg ccacgcggcc gctcaagtcg
     5101 accacggaac tacgccagca tctgacgcag caggcgctgg gccgcggtga aaaacccaag
     5161 ccggtggaca tcgaccgcgc cgtgtcggcc ctgctggccg aacgcgaaat ccgcgacaac
     5221 ctggcacgcc tgcgggccaa gggctgcatc gtcgattaca ttgcctgcga tgtgcgcgac
     5281 gaacgcagcc tgggccaggt gatcgatgac gtttacgcca agcacggccg ccttgacggc
     5341 gtgatccacg gcgccggcgt gatcgaggac cgcctgatcc gcgacaagac cgaggcgtcg
     5401 ttcgcgcggg tgttcggcac caagctcgac ccggtattcg tgctgcagtc caggctcaag
     5461 gccgacagcc tgaagttcct cgcgctgttc gcctcggtcg ccggccgcta cggcaaccgc
     5521 ggccagtccg actacgcggc cgccaatgaa gcgctcaacc gcctggcctg gtcgctgtcg
     5581 cgcgcctggc cgcagacgcg cgtgatggcg atcaactggg gcccctggga tgcgggcatg
     5641 gccagcgacg gcgtgaagaa gcagttccgc gagcgcggca tcgagcccat cgctgtcgcc
     5701 gccgggcgcc gcttcttcgt cgaggaactc gagttcggct cgcgcaacca cgtcgaactg
     5761 gtggctggtg aaggcccctg gggtgccgtc accagcgaac cgccggcggc agtgcccgcc
     5821 gcgctgtccg tgccgctggt gcgcggcgac ctgcgcctgg ggccgggcgg cagcatgacg
     5881 cttgaccaca cctactcgct gggcagcgac ccttacctgg ccgatcacaa gctcgacggc
     5941 aaaccggtgt tgccggcgac cggcgcgctg gaatggatcg cgcagtttgc cgccgcggcc
     6001 tggccgacct ggcaggtctc ggagatccgc gacctgcgcg tgctcaacgg catcgtgctg
     6061 gatggcggcg gcgaccgcaa cgtgcaactg cgcgcgcgcg cctcgaccca cagcgatccg
     6121 ggcgagcagg ccatcaccgt cgagattgtc gacctggtgc gcaagctgcc gtgttatcgc
     6181 gccacggtca tcctcgccga acagctggcc atggccgcgg cgcagacgcc gagcgccatc
     6241 ggcggcacgc catttgatcc ggcacgggcc taccgcgaat acctgttcca
//
LOCUS       DASZZC010000810         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_768683,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000810 DASZZC010000000
VERSION     DASZZC010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768683"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1691)
                     /locus_tag="VHE37_17465"
     CDS             complement(204..1691)
                     /locus_tag="VHE37_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT37379.1"
                     /translation="MPESRVLLIDNDAQRAQTTAAILQFIDYSPVIAACAAELPAERR
                     PQDFLALMLLGTDDWAALGKYIEALKRDRHHPPIIVAAQHHEKLCEQFGLDRSACMSL
                     DFPIRYTQLNDLFRRASLLRMDQHAAAVRAASGPTGNSTAIRRVRRMIAQVAPFETTV
                     LVTGESGTGKEVAARAIHDQSPRRDKPFVAINCGAIPAELLESELFGHEKGAFTGAIT
                     TRKGRFEMADGGTLFLDEIGDMSLPMQVKILRVLQERTYERVGSNRSVRCDVRIIAAT
                     HRNLEESIIKGTFREDLFYRLNVFPVEIPALRERMEDLPMVIDDLVASLERSGHGSTR
                     MAPDAINALRAYHWPGNVRELSNLIERMAVLHPHTLVQAADLPARYRAGIAAAPAREE
                     MLAPPMAVAVNADTSPAFIESPLPAGSASSRVDLAQLPPDGVDLKDHIANIEINLIRQ
                     ALEQSGGVVAHAAKLLNTRRTTLVEKLRKYGLTREENEAAEQTVF"
     gene            complement(1684..2046)
                     /locus_tag="VHE37_17470"
     CDS             complement(1684..2046)
                     /locus_tag="VHE37_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT37380.1"
                     /translation="MDDNQAFLVLAKELLADSVPALSIIATGHDGYDAVRLAERHTPD
                     LIIMDLFMPSMGGLQATRLIKAQDRPPLVLIASHYDDAKHREHCAQAGADGFIGKQDY
                     EVKVAEFVSQLVGSEAHA"
     gene            complement(2096..3565)
                     /gene="rpoN"
                     /locus_tag="VHE37_17475"
     CDS             complement(2096..3565)
                     /gene="rpoN"
                     /locus_tag="VHE37_17475"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HVT37381.1"
                     /translation="MKPALHLHTGQHMTMTPQLLQSIRLLQLSSLELEQELRQALEKN
                     VMLEEAEEEEEESAAELAQEEAAPLKATQEDAAVEVAGDDPAAAQKVEIDYDATNSEA
                     WSGGEPPDEEGESLEARTAAPQSADARVQALAQLELVVSSRREAALVGAIVEAIDDNG
                     YLEQLLDEIRAQLPEELGATAAEMEAALALVQGVEPTGFGARTLSECLLLQIRALPAA
                     TPGRDVAEYIASACLERLAGGDLKEVRELLGVDAQAMTQAVDLILSLNPKPGASLGSP
                     AQAVVPDLIVSDHRGSWKVELNPANLPRVRINGTYERMLSGVAHRAMRDQLAEARWLV
                     RGIEMRNDTLLKTARVIFERQRGFLARGEEGMAPLNLREVADAIAMHESTVCRVTTNK
                     YVQTPWGVYELKAFFPSHISGAAGETSGTAVKAMIRRIIDTEGRRQPLCDGDIAAILL
                     RNGVHVARRTVAKYREAMKIAPAKERRTRGQRAEQRMAV"
BASE COUNT          500 a         1221 c         1229 g          646 t
ORIGIN      
        1 atacggttgt ccgctggcgg tggagcaagt cctgtgcctc gcgcgggcgg tgctgcaacg
       61 gagcggatct ggagcgcatc gggacaaggt ctccccatgg cctcctcgcg ggaggggccg
      121 gcatggatgg atacgacgga agtggcggtg acgggtggcg gcgacggaat tccgtacgcc
      181 aaatctccgt caccggcggc gcatcagaaa accgtctgct cggccgcttc gttttcctcg
      241 cgcgtcaggc cgtacttgcg cagcttctcc accagcgtgg tgcggcgcgt gttgagcagc
      301 ttggccgcat gcgccaccac gccgccggac tgctccagcg cctggcggat caggttgatc
      361 tcgatgttgg cgatgtggtc cttcaggtcg acgccatccg gcggcagctg cgccaggtcc
      421 acgcgggaac tggcgctgcc ggcgggcagc ggtgactcga tgaacgcggg cgaggtgtcg
      481 gcgttgaccg ccaccgccat cggcggtgcc agcatttcct cgcgtgccgg tgcggccgcg
      541 atgccggcgc ggtagcgcgc cggcagatct gccgcctgca ccagcgtgtg cgggtgcagc
      601 accgccatgc gctcgatcag gttggacagc tcgcgcacat tgcccggcca gtggtaggcg
      661 cgcagcgcgt tgatcgcgtc cggcgccatg cgggtggaac cgtggccgct gcgctcgagg
      721 ctggcaacca ggtcatcgat gaccatcggc aggtcttcca tgcgctcgcg cagcgcgggg
      781 atttccaccg ggaacacgtt caggcggtag aacaggtctt cgcggaaggt gcccttgatg
      841 atcgactcct cgaggttgcg atgcgtcgcc gcgatgatgc gcacgtcgca gcgcacgctg
      901 cggttgctgc cgacgcgctc gtaggtgcgc tcctgcagca cgcgcaggat cttcacctgc
      961 atcggcaggc tcatgtcgcc gatctcgtcg aggaacaggg tgccgccgtc ggccatttca
     1021 aaacggccct tgcgggtggt gatggcgccg gtgaacgcgc ccttctcgtg gccgaacagc
     1081 tcgctctcca gcagctcggc cggaatggcg ccgcagttga tcgccacgaa aggcttgtcg
     1141 cggcgcggcg actggtcgtg gatggcgcgc gccgccactt ccttgccggt gccggactcg
     1201 ccggtcacca gcaccgtggt ctcgaacggc gccacctgcg cgatcatgcg gcgcacgcgg
     1261 cggatggccg tgctgttgcc ggtggggccg gacgcggcgc gcacggcggc ggcgtgctgg
     1321 tccatgcgca gcaggctggc gcggcggaac aggtcattga gctgcgtgta gcggatcggg
     1381 aaatccagcg acatgcaggc gctgcggtcg agcccgaact gttcgcacag cttctcgtgg
     1441 tgctgcgcgg cgacgatgat cggcgggtga tgccggtcgc gcttgagcgc ctcgatgtac
     1501 ttgcccagcg cggcccagtc gtcggtgccc agcagcatca gcgccagaaa atcctgcgga
     1561 cgccgctcgg cgggcagctc ggcggcgcag gccgcgatca ccgggctgta gtcgatgaac
     1621 tgcaggatcg cggcagtggt ctgcgcgcgc tgtgcatcgt tgtcgatcag taatacgcgc
     1681 gactcaggca tgcgcctcgc tcccaaccag ttgactcacg aattccgcaa ctttcacttc
     1741 gtaatcctgc ttgccgatga agccgtcggc gccggcctgc gcgcaatgct cgcggtgctt
     1801 ggcgtcgtcg taatggctgg cgatcagcac cagcggcggc cggtcctgcg ccttgatcag
     1861 gcgcgtcgcc tgcaggccgc ccatgctggg catgaacaaa tccatgatga tcaggtccgg
     1921 cgtgtggcgc tcggccagcc gcaccgcgtc gtagccgtca tggccggtgg cgatgatcga
     1981 aagtgcaggc acgctgtcgg ccagcagttc tttggccagc accagaaagg cctgattgtc
     2041 gtccaccaaa atcgtcctga tacccatatc gctcctccat cattccgtcg acagttcaca
     2101 ccgccatgcg ctgttcggcc cgctggccgc gcgtacggcg ttccttggcg ggcgcaatct
     2161 tcatcgcctc gcggtacttg gccaccgtgc ggcgcgccac gtgcacgccg ttgcgcagca
     2221 atattgccgc gatatcgccg tcgcacaggg gctgacgccg accctcggtg tcaatgatcc
     2281 gacgaatcat ggccttcacc gcggtgcccg aggtctcgcc ggccgcgccg gaaatatggc
     2341 tcgggaagaa ggccttcaat tcatacacac cccatggggt ctgcacgtac ttgttggtgg
     2401 tcacgcggca cacggtggac tcgtgcatgg cgatggcgtc ggccacctcg cgcaggttga
     2461 gcggcgccat gccttcctcg ccacgcgcca ggaagccgcg ctggcgctcg aagatcacgc
     2521 gcgcggtctt cagcagggtg tcgttgcgca tctcgatgcc gcgcaccagc cagcgcgctt
     2581 cggccagctg gtcgcgcatg gcgcggtgcg ccacgccgct gagcatgcgc tcgtaggtgc
     2641 cgttgatgcg cacgcgcggc aggttggccg ggttcagctc cactttccag ctgccgcggt
     2701 ggtcggacac gatcaggtcc ggcaccaccg cctgcgccgg gctgcccagc gaggcgcccg
     2761 gtttcggatt gagcgacagg atcaggtcga ccgcctgcgt catcgcctgc gcgtcgacgc
     2821 cgagcagttc gcgcacttcc ttgagatcgc cgccggccag ccgttcgagg caggcgctgg
     2881 cgatgtactc ggccacgtca cggccgggcg tggccgccgg cagcgcgcgg atctgcagca
     2941 gcaggcattc ggacagggtg cgcgcgccga agccggtcgg ctccacgccc tgcaccagcg
     3001 ccagcgccgc ttccatctcc gccgcggtgg cgccgagttc ttcgggcagc tgcgcgcgga
     3061 tctcgtcgag caattgttcg agatagccgt tgtcgtcgat cgcctcgacg atggcgccga
     3121 ccagcgcggc ttcgcgccgg ctgctgacca ccagctcgag ctgcgccagc gcctgcacgc
     3181 gcgcgtcggc tgactgcggc gcggcggtgc gcgcctccag gctttcgccc tcctcgtccg
     3241 gcggctcgcc gccgctccat gcttcggaat tcgtagcgtc gtaatcgatc tcgaccttct
     3301 gcgccgccgc cggatcgtcg ccggccactt cgaccgcggc gtcttcctgc gtggccttga
     3361 gcggggcggc ctcttcctgc gcaagttccg cggccgactc ctcctcttcc tcctcggcct
     3421 cctccagcat cacgttcttc tccagcgcct ggcgcagctc ctgctcgagt tcgagcgagg
     3481 acagctgcag caggcggatg gactgcagca gctgcggcgt catggtcatg tgctgaccgg
     3541 tgtgcaggtg cagggctggc ttcatggttc ggtctcctcg tgcgcgttgt tggagc
//
LOCUS       DASZZC010000811         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_768890,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000811 DASZZC010000000
VERSION     DASZZC010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768890"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VHE37_17480"
     CDS             complement(<1..291)
                     /locus_tag="VHE37_17480"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HVT37382.1"
                     /translation="MSRKLVLLRHGQSQWNLDNRFTGWVDVDVTAQGEAEARNAGRLM
                     LEEGLAFDVAHTSVLKRAIRTLNLALDVMYQSWIQVRKDWRLNERHYGDLQGL"
     gene            complement(322..951)
                     /locus_tag="VHE37_17485"
     CDS             complement(322..951)
                     /locus_tag="VHE37_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HVT37383.1"
                     /translation="MSWNRDEMAQRAARELRDGYYVNLGIGLPTLVANFIPAGMDVWL
                     QSENGLLGIGPFPKENEVDADLINAGKQTITTVPGSAFFSSADSFGMIRGGHINLSIL
                     GAMQVTDQGDIANWMIPGKMVKGMGGAMDLVAGVKNVVVLMEHTAKGGEHKILGKCTL
                     PLTGVGVVNRIITELCVLDVTPQGLKLVELAPGVTAEEVKQKTGAPVAA"
     gene            complement(948..1643)
                     /locus_tag="VHE37_17490"
     CDS             complement(948..1643)
                     /locus_tag="VHE37_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HVT37384.1"
                     /translation="MNKLYPSAAAALAGIVQDGQTLAVGGFGLCGIPEALIAALRDSG
                     AKNLTVISNNAGVDGFGLGQLLTTRQIKKMISSYVGENKEFERQFLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGYGTVVAEGKEIREFDGEKYVMERSLRADVSLVKAWKA
                     DKSGNLIFRKTARNFNPMVATAGRVTVVEAEQIVEVGALDPDQVHTPGIFVKRIVHNP
                     TPEKRIEQRTVSK"
     gene            complement(1692..>2558)
                     /locus_tag="VHE37_17495"
     CDS             complement(1692..>2558)
                     /locus_tag="VHE37_17495"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HVT37385.1"
                     /translation="EVGPRDGLQNEKQLVPAAVKIGLIDRLTDCGYAVIEATSFVSPK
                     WVPQMADAAEVLAGIRRRPGVSYPVLVPNLQGLERALAAGVEEIAVFTAASDAFNQKN
                     INATVAESLERLKPVVEQALAKNLRVRAYVSTVLGCPYQGEVALNEVVRVTRAMVDMG
                     CYEISLGDTIGIGTPVKAREMFRACADAVGAEKLAAHFHDTRGQALANTLACLEEGCR
                     VVDASVSGLGGCPYARGASGNLASEDLLYLLHGMGFDTGIDLPRVIETSRWMSAQLAR
                     APGSKMARIEHS"
BASE COUNT          380 a          875 c          799 g          504 t
ORIGIN      
        1 caggccctgc aggtcgccgt agtggcgttc gttcaggcgc cagtccttgc gtacctggat
       61 ccagctctgg tacatcacgt ccagcgccag gttcagcgtg cggatcgcgc gcttgagcac
      121 cgaggtgtgc gcgacgtcga acgccaggcc ttcttccagc atcagccggc cggcgttgcg
      181 cgcctcggct tcgccctgcg cggtgacgtc cacatccacc cagccggtga agcggttgtc
      241 gagattccac tggctctggc cgtggcgcag caacacgagt ttgcgagaca tctttcttcc
      301 tctttccctt tctgccgcgg gctacgcggc taccggcgcg ccggtcttct gcttcacttc
      361 ttcggccgtc acccccggcg cgagttccac cagcttgagt ccctgcggcg tcacgtcgag
      421 cacgcagagt tccgtgatga tgcggttgac cacgcccacg ccagtcagcg gcagcgtgca
      481 cttgcccagg atcttgtgct cgccgccctt ggcggtgtgc tccatcagca ccaccacgtt
      541 cttcaccccg gccaccaggt ccatggcgcc gcccatgccc ttgaccatct tgcccgggat
      601 catccagttg gcgatgtcgc cctggtcggt cacctgcatg gcgccgagga tggacaggtt
      661 gatgtggccg ccgcggatca tgccgaacga atcggcgctg gagaagaacg cgctgcccgg
      721 cacggtggtg atggtctgct tgccggcgtt gatcaggtcg gcgtccactt cattttcttt
      781 cgggaagggc ccgatgccga gcaggccgtt ctcgctctgc agccacacat ccatgccggc
      841 gggtatgaag ttggcgacca gcgtcggcag tccgatgcca aggttcacgt agtagccgtc
      901 gcgcagttcc ctggcggcgc gctgcgccat ctcgtccctg ttccagctca tttggacacc
      961 gtgcgctgct cgatgcgctt ttcaggagtg gggttgtgca cgatgcgctt cacgaaaatg
     1021 cccggcgtgt ggacctggtc gggatcgagc gcgcccacct cgacgatctg ctccgcctcg
     1081 accacggtga cgcggccggc ggtggcgacc atcgggttga agttgcgcgc cgtttttctg
     1141 aatatcaggt tgccgctctt gtccgctttc caggccttga ccagcgacac gtcggcgcgc
     1201 agcgaacgtt ccatcacgta tttctcgccg tcgaattcgc ggatctcctt gccctccgcc
     1261 accaccgtgc cgtagccggt cttggtgaag aacgcgggta tgccggcgcc gccggcgcgc
     1321 agcttttcgg ccagcgtgcc ctgcggagtg aattccagtt cgagttcacc ggccaggaac
     1381 tggcgctcga actccttgtt ctcgcccacg tacgacgaga tcattttttt gatctgccgc
     1441 gtggtcagca gctggccgag gccgaagccg tcgacgccgg cgttgttgga aatcaccgtg
     1501 agattctttg cgccgctgtc gcgcagtgca gcgatcagcg cctcggggat gccgcacagg
     1561 ccgaaaccgc ccaccgcgag cgtctggccg tcctggacaa tgccggccag tgcagcggcg
     1621 gcactgggat acaacttgtt cactcgtgaa cctctctcgc tggaaatcga ccgctgcgcg
     1681 gcctggaata gttatgagtg ttcgatgcgc gccattttac tacccggcgc gcgcgccagt
     1741 tgcgccgaca tccagcgcga ggtttcgatc acgcgcggca ggtcgatgcc ggtgtcgaag
     1801 cccatgccgt gcagcaggta cagcaggtct tcgctggcga gattgccgct ggcaccgcgc
     1861 gcgtacgggc agccgcccag gcccgacacg ctggcgtcga ccacgcggca gccttcttcg
     1921 agacaggcca gcgtgttggc cagcgcctgc ccgcgcgtgt cgtggaaatg cgcggcgagt
     1981 ttttccgcgc ccaccgcatc ggcgcaggcg cggaacattt cgcgcgcctt caccggcgtg
     2041 ccgatgccga tggtgtcgcc gagggaaatt tcatagcagc ccatgtcaac cattgcgcgt
     2101 gtcacgcgca cgacctcatt cagggcaacc tcgccttgat agggacagcc gagcacggtg
     2161 gacacgtacg cgcgcacgcg caggtttttc gccagcgcct gctcgaccac cggcttgagc
     2221 cgttccagcg attcggccac ggtggcgttg atgttcttct ggttgaaggc gtcactggcg
     2281 gcggtgaaca ccgcgatctc ctccacgccg gcggccagcg cgcgctccag gccctgcagg
     2341 ttgggcacca gcaccggata gctcacgccg ggcctgcgcc ggatgccggc gagcacttcg
     2401 gcagcgtcag ccatctgcgg cacccacttg gggctgacga agctggtggc ctcgatcacc
     2461 gcatagccgc aatcggtcag ccggtcgatc agcccgatct tcaccgccgc cggcaccagt
     2521 tgcttctcgt tctgcaggcc gtcgcgcggc cccacttc
//
LOCUS       DASZZC010000812         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_769233,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000812 DASZZC010000000
VERSION     DASZZC010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769233"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VHE37_17500"
     CDS             complement(<1..1466)
                     /locus_tag="VHE37_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HVT37386.1"
                     /translation="MTDAAARGRSALLGQKMTAAEVADASARGWVAILLGKLSSHAIQ
                     LLTLVVIARLLAPSDFGVLTMVMAVIGIANIFQDLGLAAAIVREPVLQHSQVNTVFWI
                     NLSMGCAMTLAGCLAAPAIRTFYDDARVEQVALALSFNFLIGALGAQQFALLRRNLRF
                     RELARTSVAATMLGNVVALICAWRGAHYWALVASTLSSSTFSTLVAWRLTGWRPSLPQ
                     FDPSVRRMLSFGGYLVVFALLRYAGTNVQNILLGRTINSAAVGHFNRGFQLLQLTEGY
                     VVDPIDLTARTAMSKMLDDAPAFSDYYLRQASLLCALAAPIGFCTTVLAGDIIAVLFG
                     PAWTSSAQVLRIVGYGVIARVLCVSTGWVFVARGDSRRMMRWGVVGWGALIACLLMAA
                     PYGIEGMAWAYTASIWLLLVPCLAYAFRGTSLRVADVARRLMPIVAAGGLACAGLYLL
                     WLMLAPLPALARLPLCGTVYLALYAGLLAWPFGQRQLFR"
     gene            complement(1489..2250)
                     /locus_tag="VHE37_17505"
     CDS             complement(1489..2250)
                     /locus_tag="VHE37_17505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HVT37387.1"
                     /translation="MRRLFRKKTAKTPAVPKQTSGELEDLTREYGALYQKVRPYTMTG
                     LPRIAALAEAVRYVVKRQLPGAFAECGVWRGGSVLAMITVLQELGCDDRDIYLYDTFE
                     GMTEPTAEDTSRFDGSANETWKQAQVTGERPWGALFSAERFGEEQVRQMLEASGYPKH
                     RLHLVRGPVESTIPAVAPEQIALLRLDTDWYESTRHELLHLYPRLCASGVLIIDDYGH
                     WEGCRRAVDEYFGPGGAAPLLLQRIDYTGRLAIKC"
     gene            complement(2281..>3195)
                     /locus_tag="VHE37_17510"
     CDS             complement(2281..>3195)
                     /locus_tag="VHE37_17510"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVT37388.1"
                     /translation="LIVAGTYRSGTTSLYTYLSGHPQVQPSSIKEPAFFFSERWAETP
                     PVYPPGHEVEAYLSLFKRKPQGNTWLEATPNYLHDPGCAERIRAALPQAKIVVVLRDP
                     VSRLVSWYKHSLFQGRLGASSNFAEWIGGQLADPRPEETRPYMQRAVVHGRYSRYLEE
                     YYRVFGRANVRVVWFDDLEANPRQVMQDICGFAGIDPAYFDDYRFKAQNQSMQFNRRW
                     LYNAYMRVCRLLVAASAPLPRLQFQVKKVVYSIAPAFLRWVTRPADEVDMPEATRLAL
                     VEYYRQDIEPLRQVLGTKIPWAGAYQPR"
BASE COUNT          614 a         1066 c         1026 g          489 t
ORIGIN      
        1 cggaacagct ggcgctgtcc gaagggccag gccagcagcc cggcatacag cgcaagatag
       61 acggttccgc acagcggcaa gcgcgcgagg gccggaagcg gcgccagcat cagccacaag
      121 agatacaggc cggcgcacgc aagcccgccc gcggcgacga tcggcatcag ccggcgcgcg
      181 acatcagcca cgcgcagcga ggtgccgcgg aacgcgtagg ccagacaggg aaccagcagc
      241 agccagatgc ttgcggtgta ggcccaggcc ataccctcga tgccataggg tgccgccatc
      301 agcagacatg cgatcagcgc gccccacccc accacgcccc agcgcatcat gcgccgcgaa
      361 tctccgcgcg cgacgaagac ccagccggtg ctgacgcaaa ggacccgggc aatcacgccg
      421 tagccgacga tgcgcagcac ctgcgcggaa ctggtccacg ccgggccgaa cagcacggcg
      481 atgatgtcgc ccgcaagcac ggtagtgcag aagccgatcg gcgcggcgag cgcgcacagc
      541 aggctcgcct ggcgcaggta gtagtcggag aaggccggcg catcgtcgag catcttcgac
      601 atggcggtgc gcgcggtgag gtcgattgga tcgaccacgt aaccctcggt cagctgcagc
      661 agctggaacc cccggttgaa atggcccacg gccgcggaat tgatggtgcg cccgagcagg
      721 atgttctgca cattggtgcc ggcataacgc agcagcgcga acacgaccag gtagccgccg
      781 aaggacagca tgcgccgcac gctgggatcg aattgcggca ggctcggccg ccagccggtg
      841 agccgccatg ccaccagcgt cgagaaggtg gatgacgaca acgtcgaagc caccagcgcc
      901 cagtagtgcg cgccgcgcca cgcgcagatc agcgcaacca cgttgcccag catggtcgcc
      961 gccacactgg tccgcgccag ttcgcggaag cgcaggttgc gtcgcagcag cgcaaactgc
     1021 tgtgcaccga gtgcaccaat caggaaattg aacgacagcg ccagcgccac ctgctccacc
     1081 cgcgcatcgt cgtaaaaggt gcggatggcc ggggccgcca ggcagcctgc cagcgtcatc
     1141 gcgcaaccca tggacaggtt gatccagaag acagtgttga cctgcgagtg ctgcagcacc
     1201 ggctcgcgca cgatggccgc ggccaggccc agatcctgga agatgttggc gatgccgatc
     1261 accgccatca ccatggtcag cacgccgaaa tcggacggcg ccagcaggcg ggcgatcacc
     1321 accagcgtga gcaactggat ggcgtgcgaa ctgagcttgc ccagcaggat ggcgacccag
     1381 ccgcgtgccg aggcgtcggc gacttccgcc gccgtcattt tctggccgag caaggcggat
     1441 cggccgcgcg cggctgcgtc ggtcacttca gatccgtccg ctgccgcttc agcacttgat
     1501 ggccagcctg ccggtgtagt caatccgttg cagcagcagc ggcgcggcac cgccggggcc
     1561 gaaatactcg tcgaccgccc tgcggcagcc ttcccaatgg ccgtagtcgt cgatgatcag
     1621 gacgccggac gcacacaagc gcggatacag gtgcaacagt tcgtggcgcg tcgattcgta
     1681 ccagtccgtg tcgagccgca gcagggcgat ctgctccgga gccacggccg ggatggtgct
     1741 ctccaccgga ccccgcacca gatgcaggcg gtgcttcgga tagccgctgg cttcgagcat
     1801 ctgccgcacc tgttcttcgc cgaaacgctc agcgctgaac agcgcgcccc agggacgttc
     1861 gccggtgacc tgcgcctgct tccaggtttc attggccgaa ccatcgaagc gcgaggtgtc
     1921 ctccgcggtc ggctcggtca tgccctcgaa ggtgtcgtag agatagatgt cgcggtcgtc
     1981 gcatccgagt tcctgcaaca cggtgatcat cgccagcacc gaaccgccgc gccagacgcc
     2041 gcactcggca aatgcgccgg gcagctggcg cttgaccacg taacgcacag cctcggccag
     2101 cgccgcgatg cgcggcaggc cggtcatcgt gtacggccgc accttctggt acagggcgcc
     2161 gtattctcgc gtcaggtcct ccagttcgcc cgatgtctgc ttcggcacgg ccggagtctt
     2221 ggcggttttt tttcggaaca ggcgtcgcat ggctgcaggt atggatgatc ggtagggaaa
     2281 ctagcggggc tggtaagcgc cggcccaggg tattttagtg ccaagcacct gccgcaacgg
     2341 ctcgatgtcc tgtcggtaat actccaccag cgccagccgc gtcgcttcgg gcatgtcgac
     2401 ctcgtcggcg ggcctggtca cccagcgcag gaaagccggc gcgatcgagt aaaccacctt
     2461 cttcacctgg aactgcagcc tgggcagcgg tgcgctggct gccaccagca aacggcatac
     2521 ccgcatgtag gcgttgtaca gccagcggcg gttgaactgc atcgactggt tctgcgcttt
     2581 gaacctgtag tcatcgaagt aggccggatc aatcccagca aagccgcaga tgtcttgcat
     2641 cacctgccgc ggattggctt cgagatcatc gaaccagacc acgcgcacat tggcgcgccc
     2701 gaatacccgg taatactcct cgaggtagcg cgaataccgt ccatgaacga cggcccgttg
     2761 catgtacggc cgtgtctcct ccggccgcgg gtcggcaagt tgcccaccga tccactcggc
     2821 gaaattgctg ctggccccca gccggccctg gaacagggaa tgcttgtacc acgacaccag
     2881 gcggctcacc gggtcgcgca ggaccaccac gatttttgct tgcggcaacg ccgcccggat
     2941 acgctctgca cagccgggat cgtgcaggta gttcggcgtc gcttccagcc aggtgttgcc
     3001 ctgcggcttg cgcttgaaca gcgaaaggta agcttcgact tcgtgcccgg gcggatacac
     3061 cggcggcgtt tcagcccagc gctccgaaaa aaaaaatgca ggctccttga tgctcgaagg
     3121 ctgtacctgc ggatgtcccg acaggtaggt atagagcgag gtcgtgcctg agcgataggt
     3181 gccggccacg atcag
//
LOCUS       DASZZC010000813         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_896451,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000813 DASZZC010000000
VERSION     DASZZC010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896451"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2045)
                     /locus_tag="VHE37_17515"
     CDS             complement(114..2045)
                     /locus_tag="VHE37_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT37389.1"
                     /translation="MDVMLWHLLQWTAGLLLLALFVRVSRVIVFIDNGSIGVVEKLWS
                     LRGSIKSGFIALEGSAGFQPEILRGGVHFFPPFQYRIHVRPLPAVPQGTIGYVFARSG
                     SALPPGQALGSLPAGVGFEDARAFIGQGGQRGPQRQILREGVYPINIAQFVILTSEGT
                     HAISLDDDHKAIEVMRACIEAREGFAPVVLRDREDLVGVVTVHDGPSLDHGQIIATSV
                     GTNPADAARYHNCFQDPERFLAAGGRRGRQEQVLVEGTYYLNCLFATVERAPKTVVDI
                     GKVGVVVSYTGRDGSDLTGSEYKHGELVEQGCRGVWERPLQSGKYAFNPHAIKVIMVP
                     TTNFVLRWVAGSSEEHGYDSNLEEVRLITSDAFEPMLPLSIVVQIPYDAAPRVVQQFA
                     DLKLLVEQTLDPMVSAYFKDAAQKCTLLELVQQRAEIQKKAFAEMKERFARYHLNLME
                     VMIGTPRPAAGDQHMATILDQLRARQVATEQAITYDNQRKAAEAEREMREAEAAAKAQ
                     TGLTQSKIGIEIAENTGRANVAIATREADSVRVKAAGNAERIRVEGLADAARIEAVGR
                     ANAASVEAQVKAFGGPEFQLRKEIAQMLQLAIDGAKVPLVPTVVVGEHDGGSALQALL
                     AMALGDKAVAARGTALTQQ"
     gene            2212..2475
                     /locus_tag="VHE37_17520"
     CDS             2212..2475
                     /locus_tag="VHE37_17520"
                     /inference="COORDINATES: protein motif:HMM:NF039792.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6471 domain-containing protein"
                     /protein_id="HVT37390.1"
                     /translation="MSNHPDALEYVKALLRAEKARSGLTWSAISAQLDRLGVKQSASN
                     LSTKASRGNMSAPLFLAILKITGADELNLRRLELHKAAPRKRR"
BASE COUNT          401 a          889 c          825 g          423 t
ORIGIN      
        1 taacaagcga agcaacggtg cggtggaggc attctggcac cgaatgcgct gcgcattcgc
       61 gccagcaaaa acggcgcgcg cagcacaaag cccgcgcgcg ccgttgccac tgcctactgc
      121 tgcgtgagcg cagtaccacg cgcggcgacg gccttgtcgc ccagcgccat ggccagcagc
      181 gcctgcagcg cggagccgcc gtcgtgctcg cccaccacca cggtcggcac cagcggcacc
      241 ttggcgccgt cgatggccag ctgcagcatc tgcgcgatct ccttgcgcag ctggaactcg
      301 ggcccgccga atgccttcac ctgcgcttcc accgacgccg cgttggcgcg gcccaccgcc
      361 tcgatgcgcg cggcatcggc caggccttcc acgcggatgc gctcggcgtt acccgccgcc
      421 ttcacccgca cgctgtcggc ctcgcgcgtg gcgatggcga cgttggcgcg gccggtgttc
      481 tcggcgatct cgatgccgat cttgctctgc gtgagcccgg tctgcgcctt ggctgcggcc
      541 tcggcttcgc gcatctcgcg ctcggcctcg gcggccttgc gctggttgtc gtaggtgatg
      601 gcctgctcgg tcgccacctg gcgcgcgcgc agctggtcga ggatggtggc catgtgctgg
      661 tcgccggccg cggggcgcgg cgtgccgatc atcacctcca tgaggttgag gtggtacctg
      721 gcgaagcgct ccttcatctc ggcaaaggcc ttcttctgga tttccgcgcg ctgctgcacc
      781 agctcgagca gcgtgcattt ctgcgcggcg tccttgaagt aggcggacac catcgggtcc
      841 agcgtctgct ccaccagcag cttgagatcg gcgaactgct gcaccacgcg cggcgcggcg
      901 tcgtacggga tctgcacgac gatggacagc ggcagcatcg gctcgaaggc gtcgctggtg
      961 atcagccgca cctcttcaag attgctgtcg tagccatgct cctcgctgct gccggccacc
     1021 cagcgcagca cgaagttggt ggtcggcacc atgatcacct tgatcgcgtg cgggttgaac
     1081 gcgtacttgc ccgactgcag cggccgctcc cacacgccgc ggcagccctg ctccaccagt
     1141 tcgccgtgct tgtactcgct gccggtgagg tcggagccgt cgcggccggt gtacgacacc
     1201 accacgccca ccttgccgat gtcgaccacc gtttttggcg cgcgctccac cgtggcgaac
     1261 aggcagttca ggtaataggt gccttcgacc agcacctgct cctggcggcc gcggcggccg
     1321 ccggcggcga ggaagcgttc cgggtcctgg aagcagttgt gatagcgcgc ggcgtcggcc
     1381 gggttggtgc ccacgctggt ggcgatgatc tgcccgtgat ccagcgacgg gccgtcatgc
     1441 acggtgacca cgccgaccag gtcctcgcgg tcgcgcagca ccaccggcgc gaagccttcg
     1501 cgcgcctcga tgcacgcgcg catcacctcg atggccttgt ggtcgtcgtc caggctgatg
     1561 gcgtgcgtgc cctcgctggt gaggatgacg aactgcgcga tgttgatcgg gtacacgccc
     1621 tcgcgcagga tctgccgctg cggcccgcgc tggccgccct gcccgatgaa cgcacgcgcg
     1681 tcctcgaagc ccacgccggc cggcagcgag cccagcgcct ggcccggcgg cagcgcgctg
     1741 ccgctgcgcg cgaacacata accgatggtg ccctgcggca ccgccggcag cggccgcacg
     1801 tggatgcggt actggaacgg cgggaagaaa tgcacgccgc cgcgcaggat ctccggctgg
     1861 aagccggcgg acccttccag cgcgatgaag ccggacttga tcgagccgcg caggctccac
     1921 aacttttcca cgacgccgat cgagccgttg tcgatgaaca cgatgacgcg cgacacgcgc
     1981 acgaacagcg caagcaacag caggccggca gtccattgca gcaggtgcca cagcatgacg
     2041 tccatgagtt tcccctaggt cagtcacgtc tctcattgat gacgtgtgtc acttatacat
     2101 gccttatggc acttatccgt gacgtgtttg accctacgcc ccggcaccct cgttgtcaac
     2161 aatgcgtgac gttggtagat tcgcctcaag cccgttccgc aagccgcacg catgtcgaat
     2221 catcccgatg cactggagta cgtgaaggcg ctgctgcgcg cggagaaggc gcgcagcggg
     2281 ctcacctgga gcgccatcag cgcgcaactg gacaggctcg gcgtcaagca gagcgccagc
     2341 aacctgtcga ccaaggcctc gcgcggcaac atgagcgcgc cgctgttcct ggccatactc
     2401 aagatcaccg gcgcggacga gttgaacctg cgcaggctgg agctgcacaa ggccgcgccg
     2461 cgcaaacggc gctgaagcag ccgcgcaggc aggcgaaaat ggctgcaccg cactgccatc
     2521 acggctgttg ataattat
//
LOCUS       DASZZC010000814         7584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_896735,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000814 DASZZC010000000
VERSION     DASZZC010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896735"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VHE37_17525"
     CDS             <1..883
                     /locus_tag="VHE37_17525"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT37391.1"
                     /translation="HRHRSASMFREFKHPSLNGYLALLFIGLGLWWGIHQLVLNPPGG
                     PLPVWPLVVTVLSGACLGGLFVIGPNEAQVLQFFGSYVGTARNTGLRWTIPLFSRRKV
                     SVRAHNFESGKLKVNELHGSPIEIGAIIVWQVVDTAKAVFEVEDYDAFVRTQSEAALR
                     GLASRYPYDAADHREPSLRGQHEDVMAQLVQDIQERLDRCGVAVLEARISHLAYAPEI
                     AGAMLQRQQAEAIVAARTRIVDGAVGMVEMALQQLAQKNVVHLDDERKAAMVSNLLVV
                     LCGERGANPVINAGSLY"
     gene            883..1056
                     /locus_tag="VHE37_17530"
     CDS             883..1056
                     /locus_tag="VHE37_17530"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HVT37392.1"
                     /translation="MEAPERKPFPLRIREDVLDAVRKWADDELRSVNAQIEFLLRDAL
                     KRAGREPPAARKR"
     gene            complement(1067..1567)
                     /locus_tag="VHE37_17535"
     CDS             complement(1067..1567)
                     /locus_tag="VHE37_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT37393.1"
                     /translation="MKTKHCLVIAAAAALAACATATPYQPLHDGEGFSEQKIESNRFR
                     VTFAGNSITPRKTVENYLLYRAAELTLESGYDYFIVANRDTQAQTRYTQSFGGGLGFG
                     SYWWGPRSALGVGVSDAVPSTEYEAQADFVMYKGTKPEANVSAFNAREVKTNLEAEIM
                     RPKAPG"
     gene            1785..3650
                     /gene="htpG"
                     /locus_tag="VHE37_17540"
     CDS             1785..3650
                     /gene="htpG"
                     /locus_tag="VHE37_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HVT37394.1"
                     /translation="MAVEKMQFQAETRQLLQLMVHSLYSNKEIFLRELISNASDACDK
                     LRFLALNKPELLGTDELHIELMVDKDAGTLTVKDNGVGMSHDEVIENLGTIARSGTRK
                     FLEAMSGEQKKDASLIGQFGVGFYSSFIVADKVTVLTKRSDAEGVRWESAGEGDFTVE
                     PAFKQDRGTEVILHLRSDDHEFLEPLRLRTIVRRYSDHIGLPIRMKNDKGESETLNQA
                     RALWTRPKSELKDEDYEHFYQHISHDWEKPLAWTHAKVEGNLEYTTLLYIPARAPFDL
                     FDREHGRGVQLYVKRVFIMDKAAELLPPYLRFVRGLVDSSDLPLNVSREILQGNRTVE
                     KIRGALVKRVLDLLDELAEKQPEKYEKFWNLFGQVFKEGLVEDHANQERIAKLCRFHS
                     TAETGAQKVTLADYVSRMKDGQDKIYFLTAETLAAAKNSPHLEGFAQRGFEVLLLTDR
                     IDEWVVAHLPEFSGKKLESVARASADLDSKIETPEKARHETEFKPVLERVQKALGERI
                     ESARVSARLTESPACIVAGEQGLSRRMEDMLRRAGEKVPPSRPILELNPTHPLVERLK
                     GANDDGVFADLSELLFAQAVLAEGGQLDDPAGFVKRLNRLILQQGSTGSIIVGAG"
     gene            complement(3660..4316)
                     /locus_tag="VHE37_17545"
     CDS             complement(3660..4316)
                     /locus_tag="VHE37_17545"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVT37395.1"
                     /translation="MKVAIHILAASMLGICAHAASAADAVPAGTYTLDRAHASLIFRV
                     NHMGFSNYTARFRHFDAQLSFDPAQLARSSVTVTVDARSLETDFPDPAKIDFNAMLQN
                     DQWLDTAKYPEMTWRSTQVEMTGANRMRITGALTLHGLTRPVTLDATYNGGYPGFAMD
                     PHARIGFSAHGTLKRSDFGIAYGIPAPGSNMGVGDDVQLQIEAEFSGPDWKGAPAAHP
                     "
     gene            complement(4394..4858)
                     /locus_tag="VHE37_17550"
     CDS             complement(4394..4858)
                     /locus_tag="VHE37_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HVT37396.1"
                     /translation="MRAPYAALALALLIAGCAPSFKPDHSHDDVREQVVETALAQLGR
                     PYRYGGTDPDSGFDCSGLVWYSYAQAGMKVPRSTRELMSAGTEIRLADAAPGDLLFYR
                     FSDKEPSNLHVVLYVGDGRAVHAPTTTHDVRLAEIGKPEWQRNFVTAVTLLR"
     gene            complement(4859..5467)
                     /locus_tag="VHE37_17555"
     CDS             complement(4859..5467)
                     /locus_tag="VHE37_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVT37397.1"
                     /translation="MKLYTDATTPYGRKVRVVIHELGLADKVEEQVTDPYNPPPEFLA
                     ANPLSKIPTLVTEHGEALPDSRLIVEYLLTRGRGLAPLGRGSKRWAMLRRVQVAEGLI
                     DAAVATVVEKRRPESIVYHTFLDRQAAAIRRCVDMLNLEVNALSLEQIGLAEITAGVA
                     LSYLELRLPYLEWRALHEPLSAWHTQFAQRPSMLATAPPPGS"
     gene            complement(5514..6467)
                     /gene="trxB"
                     /locus_tag="VHE37_17560"
     CDS             complement(5514..6467)
                     /gene="trxB"
                     /locus_tag="VHE37_17560"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HVT37398.1"
                     /translation="MSTHHRLVILGSGPAGYTAAVYAARANLKPVMITGLEVGGQLMT
                     TTEVDNWPGDVHGLMGPDLMARMQKHAERFDVQFIYDHVHVVDLKSKPIKLKGDQGEY
                     TCDALIITTGASAKYLGIPSEEAFRGKGVSACATCDGFFYKGQDVAVIGGGNTAVEEA
                     LYLANIANKVTVVHRRDKFKGEKILHDKLNKRVAEGKVEIVWDSTLDEVLGDASGVTG
                     MRVKNLKTGAAREIALKGVFIAIGHSPNTGIFEGQLNMSGGYIIVKGGHGGDATATSV
                     PGVFAAGDVMDHVYRQAVTSAGTGCQAALDAERYLDKLAAA"
     gene            complement(6538..7137)
                     /locus_tag="VHE37_17565"
     CDS             complement(6538..7137)
                     /locus_tag="VHE37_17565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVT37399.1"
                     /translation="MSVLVNRLAPDFKAQAVMGDGSIGELKLSDYRGKKYVALFFWPL
                     DFTFVCPSEIIAHEHRRKALEAINVQLVGVSIDSQFTHYAWRNTPVNKGGIGEVGFPI
                     VADVNHEIVRAYGCEHQDGVAFRATFLIDKNGIVQHQTVNNLPLGRNVDELVRLAEAL
                     QYTEQHGEVCPAGWKKGDAGMKPTAEGVADYLSKHAKAL"
     gene            7329..>7584
                     /locus_tag="VHE37_17570"
     CDS             7329..>7584
                     /locus_tag="VHE37_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HVT37400.1"
                     /translation="MSEQKSFLRRFFGGIWLLGVGIYRFFVLIGIVMLLVMVYGALRG
                     GAPVKIENHVALVIAPTGALVEQVDKDPGAELFEQFSDQPP"
BASE COUNT         1279 a         2415 c         2554 g         1336 t
ORIGIN      
        1 tcatcgtcac aggagcgcct ccatgttccg cgagttcaag catccttcgc tcaacggcta
       61 cctcgccctg ctgttcatcg gactgggatt gtggtggggc atccaccagc tggtgctcaa
      121 tccgccgggc gggccgctgc cggtatggcc gctggtggtc acggtgctca gcggcgcgtg
      181 cctcggcggg ctgttcgtca tcggccccaa cgaggcgcag gtgctgcagt tcttcggcag
      241 ctacgtcggc accgcgcgca acaccggcct gcgctggacc atcccgctgt tcagccggcg
      301 caaggtgtcg gtgcgcgcgc acaatttcga gagcggcaag ctcaaggtca acgaactgca
      361 cggcagcccg atcgagatcg gcgccatcat cgtctggcag gtggtggaca cggccaaggc
      421 ggtgttcgag gtggaggact acgacgcctt cgtgcggacg cagtccgaag cggcgctgcg
      481 cgggctggcc agccgctacc cgtacgatgc cgccgaccac cgcgagccct cgctgcgcgg
      541 ccagcacgag gacgtgatgg cgcagctggt gcaggacatc caggagcggc tcgatcgctg
      601 cggcgtggcg gtgctggaag cgcgcatcag ccacctggcc tacgcgccgg aaatcgccgg
      661 cgccatgctg cagcggcagc aggccgaggc catcgtggcg gcgcgcacgc gcatcgtcga
      721 cggcgcggtc ggcatggttg aaatggcgct gcagcagctg gcgcagaaaa acgtcgtgca
      781 cctggacgat gagcgcaagg ccgccatggt gtcgaacctg ctggtggtgc tgtgcggcga
      841 gcgcggcgcc aacccggtga tcaacgccgg cagcctgtat tgatggaagc gcccgagcgc
      901 aaaccctttc cgctgcgcat ccgcgaggac gtgctcgacg cggtgcgcaa gtgggccgac
      961 gacgaactgc gcagcgtcaa tgcgcagatc gagttcctgc tgcgcgatgc gctcaagcgc
     1021 gccgggcgcg agccgccggc ggcgaggaag cgctaaccag ggagcctcag cccggcgcct
     1081 tcggccgcat gatctcggcc tcgaggttgg tcttcacctc gcgcgcgttg aacgcgctca
     1141 cattggcctc gggcttggtg cccttgtaca tgacgaagtc ggcctgcgcc tcgtactcgg
     1201 tgctgggtac ggcgtcgctg acgcccacgc ccagcgcgct gcgcggaccc caccagtacg
     1261 aaccgaaacc caggccgccg ccgaagctct gcgtgtagcg cgtctgcgct tgcgtgtcgc
     1321 ggttggcgac gatgaagtag tcgtagcccg attccagcgt gagctcggcg gcgcggtaca
     1381 gcaggtagtt ctccaccgtc ttgcgcggtg tgatcgaatt gccggcgaag gtgacgcgga
     1441 agcggttgga ttcgatcttc tgctcggaga agccctcgcc atcgtgcagc ggctggtacg
     1501 gtgtggcggt ggcacaggct gcgagcgcgg cagcggcggc aatcaccagg cagtgtttgg
     1561 ttttcatggg cattgctcct ttggacctgt tgtagcggcg cgagtttgac atgccagcct
     1621 gccagaaata gctgaccgca ccctgaatga aagtgttaat aattgttgag caaaaataaa
     1681 ataagacaat aatatataac acataaaacc atgctgcggt cggcttgaaa aacgcatcga
     1741 cagcctcata cagaggccag gatttggctc aaggaaaaat aacaatggct gtcgaaaaaa
     1801 tgcaattcca ggcggagacg cgccaactgc tgcagctgat ggtgcactcg ctgtattcga
     1861 acaaggaaat cttcctgcgc gagctcatct ccaacgcgtc cgatgcctgc gacaagctgc
     1921 gcttcctggc gctgaacaag cccgagctgc tcggcaccga cgaactgcac atcgagctga
     1981 tggtcgacaa ggacgccggc accctgacgg tgaaggacaa cggcgtgggc atgagccacg
     2041 acgaggtgat cgaaaacctc ggcaccatcg cccgctcggg cacccggaaa ttcctcgagg
     2101 ccatgtcggg cgagcagaag aaggacgcct cgctaatcgg ccagttcggc gtcggcttct
     2161 actcctcgtt catcgtcgcc gacaaggtca ccgtgctgac caagcgcagc gacgccgagg
     2221 gcgtgcgctg ggaatcggcc ggcgagggcg acttcaccgt ggagcccgcg ttcaagcagg
     2281 accgcggcac cgaggtgatc ctgcacctgc gctccgacga ccatgaattc ctcgagccgc
     2341 tgcggctgcg gaccatcgtg cgccgctaca gcgaccacat cggactgccc atccgcatga
     2401 agaacgacaa gggcgaaagc gaaacgctga accaggcgcg cgcgctgtgg acgcggccga
     2461 agtcggagct caaggacgag gactacgagc atttctacca gcacatctcg cacgactggg
     2521 agaaacccct ggcctggacc catgccaagg tcgaaggcaa cctcgagtac accacgctgc
     2581 tgtacatccc ggcgcgcgcg ccgttcgacc tgttcgaccg cgagcatggt cgcggcgtgc
     2641 agctgtacgt gaagcgcgtg ttcatcatgg acaaggcggc cgaactgctg ccgccctatc
     2701 tgcgcttcgt gcgcgggctg gtggattctt ccgacctgcc gctcaacgtc tcgcgcgaaa
     2761 tcctgcaggg caaccgcacg gtggagaaaa tccgcggcgc gctggtcaag cgcgtgctcg
     2821 acctgctcga tgaactcgcc gagaagcagc cggagaaata cgagaagttc tggaacctgt
     2881 tcggccaggt gttcaaggaa ggcctggtcg aagaccatgc caatcaggag cgcatcgcca
     2941 aactctgccg cttccattcg actgccgaaa ccggcgcgca gaaggtgacg ctggcggact
     3001 acgtgtcgcg catgaaggac ggccaggaca agatctattt cctcacggcg gaaaccctgg
     3061 ccgcggcgaa gaacagcccg cacctggaag gattcgcgca gcgcggcttt gaagtgctgc
     3121 tgctcaccga ccgcatcgac gaatgggtgg tggcgcacct gccggagttc tccggcaaga
     3181 agctggagtc ggtggcgcgc gcctcggccg atctcgacag caagatcgaa acgcccgaaa
     3241 aggcgcgcca cgaaaccgag ttcaagccgg tgctggaacg cgtgcagaag gcgctgggcg
     3301 agcgcatcga gtccgcgcgc gtgtccgcgc gcctgaccga gtcgcccgca tgcatcgtcg
     3361 ccggcgagca gggcctctcg cggcgcatgg aggacatgct gcgccgcgcc ggcgagaagg
     3421 tgccgccgtc gcggcccatc ctcgagctca atcccacgca tccgctggtc gagcggctca
     3481 agggcgccaa cgatgacggc gtgttcgccg atctatctga actgctgttt gcgcaggccg
     3541 tgctggcgga aggcggccag ctcgatgacc ccgccggctt cgtcaagcgg ctcaaccggc
     3601 tgatcctgca gcaggggtcg accggcagca tcatcgtcgg cgccggctga gctgcgcgct
     3661 tacgggtgcg cggccggcgc gcccttccag tcggggccgc tgaactcggc ctcgatctgc
     3721 agctgcacat cgtcgcccac gcccatgttg ctgccgggcg caggaatgcc gtaggcgatg
     3781 ccgaagtcgg accgcttgag cgtgccgtgc gcggaaaagc cgatgcgcgc gtgtgggtcc
     3841 atggcgaagc cggggtagcc gccgttgtag gtggcgtcaa gcgtgaccgg gcgggtgagg
     3901 ccatgcaggg tgagtgcacc ggtgatgcgc atgcggttgg caccggtcat ctccacctgg
     3961 gtcgagcgcc aggtcatctc cgggtacttc gccgtgtcga gccactggtc gttttgcagc
     4021 atggcgttga agtcgatctt ggccggatcg gggaaatcgg tttcgagcga gcgcgcatcc
     4081 accgtcaccg tcacgctgga tctcgccagc tgcgccgggt cgaacgacag ttgcgcgtcg
     4141 aaatgcctga agcgcgcggt gtagttggag aagcccatgt ggttgacgcg gaagatcagg
     4201 ctggcatggg cgcggtccag cgtgtaggtg cccgccggca ccgcgtccgc tgcgctcgcc
     4261 gcgtgggcgc agatgcccag catcgaggcg gccaggatgt ggatcgctac tttcatgtca
     4321 gctcctgaag ttcgagatgt acccggtagt cgggcggcgc tgcacccgat cgacaattcc
     4381 ggcaaagatt ttttcagcgc agcaacgtca cggcggtgac gaagttcctc tgccactccg
     4441 gcttgccgat ttccgccagg cgtacgtcgt gcgtggtggt gggcgcgtgc accgcgcggc
     4501 cgtcgccgac gtaaagcacc acgtgcaggt tcgacggttc cttgtcggag aagcggtaga
     4561 acagcaggtc gccgggcgcg gcgtcggcca gtctgatctc ggtgccggcc gacatcagct
     4621 cgcgcgtgct gcgcggtacc ttcatcccgg cctgcgcgta gctgtaccag accagcccgc
     4681 tgcagtcgaa gccgctgtcg gggtcggtgc cgccgtagcg gtacggccgg ccgagctggg
     4741 ccagcgcggt ttccaccacc tgctcgcgca catcgtcgtg cgagtggtcg ggcttgaagc
     4801 tgggcgcgca acctgcgatc agcaaggcga gcgcgagtgc ggcgtagggc gcgcgcattc
     4861 agctgccggg tggcggagcc gtcgccagca tcgacggccg ctgcgcgaac tgcgtgtgcc
     4921 aggccgacag cggctcgtgc agggcgcgcc attccaggta aggcaggcgc aattccaggt
     4981 agctcagcgc gacgccggcg gtgatttccg ccagcccgat ctgttccagc gacagtgcat
     5041 tcacctcgag gttgagcatg tccacgcagc ggcggatggc ggcggcctgg cggtcgagga
     5101 aggtgtggta gacgatgctt tccggccggc gtttctcgac cacggtggcg acagcggcgt
     5161 cgatcagtcc ctccgctacc tgcacccggc gcagcatggc ccagcgcttg ctgccgcggc
     5221 ccagcggcgc cagtccgcgg ccgcgcgtca gcaggtattc gacgatcagc cgcgaatccg
     5281 gcagcgcctc gccgtgctcg gtgaccagcg taggaatctt cgacaacgga ttggcggcca
     5341 ggaattccgg tggcgggttg tacggatcgg tgacctgctc ctccaccttg tccgccagcc
     5401 ccaactcgtg aatcaccacg cgcaccttgc gcccgtaggg cgttgtggca tcggtgtaaa
     5461 gcttcatctt cacgcgaatc tcccgttcgc gtgattgtac ggattccgcc gtgctacgcc
     5521 gccgcgagtt tgtcgaggta gcgctcggcg tcgagcgcgg cctggcagcc ggtgccggcg
     5581 ctggtcaccg cctgacggta gacgtgatcc atcacgtcgc cggcggcgaa cacgcccggc
     5641 acgctggtgg cagtggcgtc gccgccatgg ccgcccttga cgatgatgta accgccgctc
     5701 atgttgagct ggccttcgaa gatgccggtg ttgggtgaat ggccgatggc gatgaacacg
     5761 cccttgagcg cgatctcgcg cgcggcgccg gtcttcaggt tcttcacgcg catgccggtg
     5821 acgccggagg catcgcccag cacttcatcg agcgtggaat cccacacgat ctcgaccttg
     5881 ccttcggcca cgcgcttgtt gagcttgtca tgcaggatct tctcgccctt gaacttgtcg
     5941 cgccggtgca ccacggtgac cttgttggcg atgttggcca ggtacagcgc ttcctcgacc
     6001 gcggtgttgc ccccgccgat taccgccacg tcctggccct tgtagaagaa gccgtcgcag
     6061 gtggcgcagg cggacacgcc cttgccgcgg aaggcttctt ccgacgggat gcccaggtac
     6121 ttggccgagg cgccggtggt aatgatcagc gcgtcgcagg tgtactcgcc ctggtcgccc
     6181 ttgagcttga tcggcttgct cttcaggtcc accacgtgca catggtcgta gatgaactgc
     6241 acgtcgaagc gctccgcgtg cttctgcatg cgcgccatca ggtccgggcc catcaggccg
     6301 tgcacgtcgc ccggccagtt gtccacctcg gtggtggtca tcagctgccc gcccacttcc
     6361 aggccggtga tcatcaccgg cttgaggttg gcgcgcgcgg cgtagaccgc ggcggtgtag
     6421 ccggcggggc cggaccccag gatgaccaga cgatggtgcg tgctcatggt tgttctcggt
     6481 gatgaaaaag gcgggaagcg gtgctgccgc ttcccgcccg gtgttgcggc gccctattta
     6541 tagggccttg gcgtgcttgg acaagtagtc ggccacgcct tcggcggtcg gcttcatgcc
     6601 ggcgtcgccc ttcttccagc cggcggggca cacctcgccg tgttgctcgg tgtactgcag
     6661 tgcttcggcc aggcgcacca gttcgtcgac gttgcggccc agcggcaggt tgttcaccgt
     6721 ctggtgctgc acgatgccgt tcttgtcgat caggaaggtg gcgcggaagg ccacgccgtc
     6781 ctggtgctcg cagccatagg cgcgcacgat ctcgtggttg acgtcggcca cgatggggaa
     6841 gcccacctcg ccaatgccgc ccttgttcac cggcgtgttg cgccaggcgt agtgggtgaa
     6901 ctgcgagtcg atggacacgc cgaccagctg cacgttgatc gcttccagcg ccttgcggcg
     6961 atgttcgtgg gcgatgattt ccgacgggca gacgaaggtg aagtccagcg gccagaagaa
     7021 cagggccacg tatttcttgc cgcggtagtc ggacagcttc agctcgccga tggagccgtc
     7081 gcccatgacg gcctgggcct tgaagtcggg ggccaaccgg ttgacgagta cgctcatgga
     7141 aaaatcctcg cggggtcttt ggaatgggcg cgcagtgtag gaaagccgcg ccgggcgggt
     7201 caaagcatat ttttaaattc cgccgatagc cgctgcctat gccgaatgtc cgccgcccgg
     7261 cgcggtcaaa ccccggcggc ggcctgccgc gtgcgatact cgccccctgc attgcggaca
     7321 cttttgagat gagcgaacag aaatccttcc tgcgccgttt cttcggcggc atctggctgc
     7381 tgggcgtggg catttaccgc ttcttcgtgc tgatcggcat cgtgatgctg ctggtgatgg
     7441 tctacggcgc gctgcgcggc ggcgccccgg tgaagatcga gaaccacgtg gcgctggtta
     7501 ttgcccccac cggcgcgctg gtggagcagg tcgacaagga cccgggcgcc gagctgttcg
     7561 agcagttctc cgaccagccg ccgg
//
LOCUS       DASZZC010000815         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nevskiaceae bacterium isolate SMAG_U6226 k141_898024,
            whole genome shotgun sequence.
ACCESSION   DASZZC010000815 DASZZC010000000
VERSION     DASZZC010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957039
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nevskiaceae bacterium (soil metagenome)
  ORGANISM  Nevskiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales;
            Nevskiaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:34:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,477
            Genes (coding)                    :: 3,471
            CDSs (with protein)               :: 3,471
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Nevskiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898024"
                     /isolate="SMAG_U6226"
                     /isolation_source="soil"
                     /db_xref="taxon:1969813"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VHE37_17575"
     CDS             <1..632
                     /locus_tag="VHE37_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT37401.1"
                     /translation="VEQRMAAASALTAELRASGAMDDTPALLDDTLFGVDQIKELVIN
                     LKNFSRLDQARNAEASLNDCLEQTLTIASSVLKNRVEIVRSYGDIPRIMCAPSQINQV
                     LLNVMTNAAQAIEHDHGQLLLETSCDGDFVQVGIQDNGKGIAPENLKKIFDPFFTTKP
                     VGQGTGLGLSISFQIIESHGGTISVSSEVGKGTRFVISLPVRAQVAAAA"
     gene            645..1565
                     /locus_tag="VHE37_17580"
     CDS             645..1565
                     /locus_tag="VHE37_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT37402.1"
                     /translation="MADKPTLLLIDDEERILRSLAMLFRGNHDLHTTTDPHEALALVS
                     RMPVHVIVSDQKMPVMRGADLLKLVKERSPSTMRILLTGYSELDAVVASVNEGEIYRF
                     VNKPWDAAELRGTVAEAANIALRLFADSARAAAAPAVSSSSGSAVLVIDEDSEVYQAV
                     KEISTAPVIWATHLGQAMEALEQHRIGVIVSELAVQGESVSSLLKLLKAQHPEIVSIV
                     LTPVQDVGQFIGLINQGQIYRLLPKPLRRGPFGMNLASALRHHDMLKGSPLHVSAHKV
                     QPLQPAASDAGVASRVLGFLSRMRTRTATG"
     gene            1644..>2786
                     /locus_tag="VHE37_17585"
     CDS             1644..>2786
                     /locus_tag="VHE37_17585"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT37403.1"
                     /translation="MTSSNGNTPKDLSRRRLLAGLAAAPAIGAIAGCGSSSDNTAAVV
                     PQPDNSGLPDPSSIGIDHIVVVMMENRSFDHMLGWVPGADGVQAGRSMRNKAGDLVQS
                     FRLSQDPAYGFQGCNFADPDHGYDGGRIEFNNGACDGFLLTDGTGANPGDHFPLGYYT
                     ADDLPFYKGAAENFTVCDRYFCGILSSTIPNRVYMHAGQTDRNENTLTVSSLDTIWDR
                     LAAKGRSGTYYSVDLPMTALWGSKHLSITQPFATFLAEAALGQLPDVSFVDPAFGFSV
                     GEGPGLSRDDHPHADVRDGQAFLSQVYNALVASPAWDKTLLIINYDEWGGFADHVPPP
                     LAPVSDDERNNISPQGNDGRLGFRTPCFLIGPRARKAFVSHQQLDPN"
BASE COUNT          482 a          987 c          890 g          427 t
ORIGIN      
        1 tggtggaaca gcgcatggcc gcggccagcg cgctgaccgc ggaactgcgc gcgagcggcg
       61 ccatggacga cacgccggcg ctgctcgatg acaccttgtt cggcgtggac cagatcaagg
      121 aactggtgat caacctgaag aacttcagcc ggctggacca ggcgcgcaac gccgaggcca
      181 gcctcaacga ctgcctggag cagacgctga ccatcgcctc cagcgtgctg aagaaccggg
      241 tggagatcgt gcgcagctac ggcgacattc ccaggatcat gtgcgcaccc tcgcagatca
      301 accaggtgct gctcaacgtg atgaccaacg cggcgcaggc catcgagcac gatcacggac
      361 aactgctgct ggagacctcc tgcgacggcg acttcgtgca ggtgggcatc caggacaacg
      421 gcaagggcat tgcgccggaa aacctgaaaa aaatcttcga cccgttcttc accaccaagc
      481 cggtcggcca gggcaccggg ctggggctgt cgatcagctt ccagatcatc gaatcgcatg
      541 gcggcaccat cagcgtgagc tcggaggtcg gcaagggcac gcgctttgtc atcagcctgc
      601 cggtgcgcgc ccaggtggcc gctgctgcat aagggaatat cgcaatggcc gacaagccga
      661 cactgctgct gatcgatgac gaggagcgca tcctgcgctc gctggccatg ctgttccgcg
      721 gcaaccacga cctgcacacc accaccgatc cgcacgaggc attggcgctg gtctcgcgca
      781 tgccggtgca cgtgatcgtg tcggaccaga agatgccggt catgcgtggc gccgacctgc
      841 tcaagctggt gaaggagcgt tcgccgtcca ccatgcgcat cctgctcacc ggctactccg
      901 agctggacgc ggtggtggcc tcggtcaacg agggcgagat ctaccgcttc gtcaacaagc
      961 cctgggacgc cgccgagctg cgcggcacgg tggccgaggc ggcgaatatc gccctgcgcc
     1021 tgttcgccga cagcgcgcgc gccgcggccg cgccggcggt gagcagcagc agcgggtccg
     1081 ccgtgctggt gatcgacgag gactccgagg tctaccaggc ggtgaaggag atctccaccg
     1141 cgccggtcat ctgggccacc cacctcggcc aggcgatgga agcgctggag cagcacagga
     1201 tcggcgtcat cgtgtcggag ctggcggtgc aaggcgagag cgtgtcctcg ctgctcaagc
     1261 tgctcaaggc gcagcacccg gagatcgtgt ccatcgtgct gacgccggtg caggacgtgg
     1321 gccagttcat cggcctgatc aaccagggcc agatctaccg cctgctgccc aagccgctgc
     1381 gccgcgggcc gttcggcatg aacctggcct ctgcgctgcg ccaccacgac atgctcaagg
     1441 gctcgccgct gcacgtcagc gcgcacaagg tgcagccgct gcagccggcg gcgtccgacg
     1501 ccggcgtcgc ctcgcgcgtg ctgggctttc tttcccgcat gcgcacgcgc accgccaccg
     1561 gctaacatcc ttccccgctg tcaacgcatt gccatgcgcg gcgcctaagc tgcgcggctg
     1621 aatatttttt ggggagacaa cgcatgactt ccagcaacgg caatacgccg aaggacctct
     1681 cgcgccgccg cctgctcgcc ggcctggccg ccgcgccggc catcggcgcc atcgccggtt
     1741 gcggcagcag ctccgacaac accgcggccg tggtgccgca gccggacaac tccggcctgc
     1801 ccgacccgtc gtcgatcggc atcgaccaca tcgtggtggt gatgatggag aaccgctcgt
     1861 tcgaccacat gctcggctgg gtgccgggcg ccgacggcgt gcaggccggg cgcagcatgc
     1921 gcaacaaggc cggcgatctg gtgcagagct tccgcctgtc gcaggacccg gcatacggat
     1981 tccagggctg caacttcgcc gatcccgacc acggctacga cggcgggcgc atcgagttca
     2041 acaacggcgc ctgcgacggc ttcctgctca ccgacggcac cggcgccaat ccgggcgacc
     2101 acttcccgct cggctactac accgccgacg acctgccgtt ctacaagggc gcggcggaaa
     2161 acttcaccgt ctgcgaccgc tacttctgcg gcatcctgtc ctccaccatt cccaaccgcg
     2221 tgtacatgca cgcgggccag accgaccgca acgagaacac gctgacggtg tcgtcgctcg
     2281 acaccatctg ggaccgcctc gccgccaagg gccgcagcgg cacctactac tcggtggacc
     2341 tgccgatgac cgcgctgtgg ggcagcaagc acctgtcgat cacccagcct ttcgccacct
     2401 tcctcgccga agccgcgctg ggccagctgc ccgatgtgtc gtttgtcgat cccgcgttcg
     2461 gcttctcggt cggcgaaggg cccggcctgt cgcgcgacga tcatccgcac gccgacgtgc
     2521 gcgacggaca ggccttcctg tcgcaggtgt acaacgcgct ggtcgccagc ccggcgtggg
     2581 acaagacgct gctgatcatc aactacgacg aatggggcgg ctttgccgac cacgtgccgc
     2641 cgccgctggc gccggtgtcg gacgacgagc gcaacaacat ctcgccgcag ggcaacgacg
     2701 gccgcctggg tttccgcacg ccgtgtttcc tgatcggccc gcgcgcgcgc aaggccttcg
     2761 tctcgcacca gcagctcgat ccgaac
//
